LOCUS       DADSYM010000001        70149 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2415
            SAMN08039579-rid11723173.denovo.001, whole genome shotgun sequence.
ACCESSION   DADSYM010000001 DADSYM010000000
VERSION     DADSYM010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039579
            Sequence Read Archive: SRR6362533
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 70149)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 70149)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980162.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 03:39:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,484
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 4,347
            CDSs (with protein)               :: 4,347
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 43 of 85
            Pseudo Genes (incomplete)         :: 37 of 85
            Pseudo Genes (internal stop)      :: 19 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..70149
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039579-rid11723173.denovo.001"
                     /strain="LGE2415"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8302 N 95.9628 W"
                     /collection_date="2015-08-24"
     gene            299..1486
                     /gene="ygeW"
                     /locus_tag="J1Y83_000001"
     CDS             299..1486
                     /gene="ygeW"
                     /locus_tag="J1Y83_000001"
                     /EC_number="2.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="knotted carbamoyltransferase YgeW"
                     /protein_id="HBA7628198.1"
                     /translation="MKTVNELIKDINSLTSHLHEKDFLLTWEQTPDELKQVLDVAAAL
                     KALRAENISTKVFNSGLGISVFRDNSTRTRFSYASALNLLGLAQQDLDEGKSQIAHGE
                     TVRETANMISFCADAIGIRDDMYLGAGNAYMREVGAALDDGYKQGVLPQRPALVNLQC
                     DIDHPTQSMADLAWLREHFGSLENLKGKKIAMTWAYSPSYGKPLSVPQGIIGLMTRFG
                     MDVTLAHPEGYDLIPDVVEVAKNNAKASGGSFRQVTSMEEAFKDADIVYPKSWAPYKV
                     MEERTELLRANDHEGLKALEKQCLAQNAQHKDWHCTEEMMELTRDGEALYMHCLPADI
                     SGVSCKEGEVTEGVFEKYRIATYKEASWKPYIIAAMILSRKYAKPGALLEQLLKEAQE
                     RVK"
     gene            1544..2740
                     /gene="dpaL"
                     /locus_tag="J1Y83_000002"
     CDS             1544..2740
                     /gene="dpaL"
                     /locus_tag="J1Y83_000002"
                     /EC_number="4.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopropionate ammonia-lyase"
                     /protein_id="HBA7628199.1"
                     /translation="MSVFSLKIDIADNKFFNGETSPLFSQSQAKLARQFHQKIAGYHP
                     TPLCALDDLANLFGVKKILVKDESKRFGLNAFKMLGGAYAIAQLLCEKYHLDIETLSF
                     EHLKNAIGEKMTFATTTDGNHGRGVAWAAQQLGQNAVIYMPKGSAQERVDAILNLGAE
                     CIVTDMNYDDTVRLTMQHAQQHGWEVVQDTAWEGYTKIPTWIMQGYATLADEAVEQMR
                     EMGVTPTHVLLQAGVGAMAGGVLGYLVDVYTPQNLHSIIVEPDKADCIYRSGVKGDIV
                     NVGGDMATIMAGLACGEPNPLGWEILRNCATQFISCQDSVAALGMRVLGNPYGNDPRI
                     ISGESGAVGLGVLAAVHYHPQRQSLMEKLALNKDAVVLVISTEGDTDVKHYREVVWEG
                     KHAVAP"
     gene            2798..4009
                     /locus_tag="J1Y83_000003"
     CDS             2798..4009
                     /locus_tag="J1Y83_000003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgeY family selenium metabolism-linked
                     hydrolase"
                     /protein_id="HBA7628200.1"
                     /translation="MAKNIPFKLILEKAKDYQADMTRFLRDMVAIPSESCDEKRVVHR
                     IKEEMEKVGFDKVEIDPMGNVLGYIGHGPRLVAMDAHIDTVGIGNIKNWDFDPYEGME
                     TDELIGGRGTSDQEGGMASMVYAGKIIKDLGLEDEYTLLVTGTVQEEDCDGLCWQYII
                     EQSGIRPEFVVSTEPTDCQVYRGQRGRMEIRIDVQGVSCHGSAPERGDNAIFKMGPIL
                     GELQELSQRLGYDEFLGKGTLTVSEIFFTSPSRCAVADSCAVSIDRRLTWGETWEGAL
                     DEIRALPAVQKANAVVSMYNYDRPSWTGLVYPTECYFPTWKVEEDHFTVKALVNAYEG
                     LFGKAPVVDKWTFSTNGVSIMGRHGIPVIGFGPGKEPEAHAPNEKTWKSHLVTCAAMY
                     AAIPLSWLATE"
     gene            4063..5448
                     /gene="hydA"
                     /locus_tag="J1Y83_000004"
     CDS             4063..5448
                     /gene="hydA"
                     /locus_tag="J1Y83_000004"
                     /EC_number="3.5.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311773.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropyrimidinase"
                     /protein_id="HBA7628201.1"
                     /translation="MRVLIKNGIVVNADGQAKQDLLIESGIVRQLGTDISPQLPCEEI
                     DASGCYVFPGGVDVHTHFNIDVGIARSCDDFFTGTRAAACGGTTTIIDHMGFGPNGCR
                     LRHQLEVYRGYAAHKAVIDYSFHGVIQHINHAILDEIPMMVEEGLSSFKLYLTYQYKL
                     NDDEVLQALRRLHESGALTTVHPENDAAIASKRAEFIAAGLTAPRYHALSRPLECEAE
                     AIARMINLAQIAGNAPLYIVHLSNGLGLDYLRLARANHQPVWVETCPQYLLLDERSYD
                     TEDGMKFILSPPLRNVREQDKLWCGISDGAIDVVATDHCTFSMAQRLQISKGDFSRCP
                     NGLPGVENRMQLLFSSGVMTGRISPERFVELTSAMPARLFGLWPQKGILAPGSDGDVV
                     IIDPRQSQQIQHRHLHDNADYSPWEGFTCQGAIVRTLSRGETIFCDSTFTGKAGRGRF
                     LRRKPFVPPVL"
     gene            5496..6428
                     /gene="arcC"
                     /locus_tag="J1Y83_000005"
     CDS             5496..6428
                     /gene="arcC"
                     /locus_tag="J1Y83_000005"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HBA7628202.1"
                     /translation="MSKKIVLALGGNALGDDLAGQMKAVKITSQAIVDLIAQGHEVIV
                     THGNGPQVGMINQAFEAAAKTEAHSPMLPMSVCVALSQGYIGYDLQNALREELLSRGI
                     NKPVATLVTQVEVDANDPAFLNPTKPIGSFFTEQEAELLTKQGYTLKEDAGRGYRRVV
                     ASPKPVDIIEKETVKALVDAGQVVITVGGGGIPVIREGNHLRGASAVIDKDWASARLA
                     EMIDADMLIILTAVEKVAINFGKENEQWLDRLSLSDAERFIEEGHFAKGSMLPKVEAA
                     ASFARSRAGREALITVLSKAKEGIEGKTGTVICQ"
     gene            complement(6469..8094)
                     /locus_tag="J1Y83_000006"
     CDS             complement(6469..8094)
                     /locus_tag="J1Y83_000006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EF2563 family selenium-dependent molybdenum
                     hydroxylase system protein"
                     /protein_id="HBA7628203.1"
                     /translation="MNIFTEAAKLEEQNCPFAMAQIVDSRGSTPRHSAQMLVRADGSI
                     VGTIGGGMVERKVIEESLQALQERKPRLFHGRMARNGADAVGSDCGGAMSVFISVHGM
                     RPRLVLIGAGHVNRAIAQSAALLGFDIAVADIYRESLNPELFPPSTTLLHAESFGAAV
                     EALDIRPDNFVLIATNNQDREALDKLIEQPIAWLGLLASRRKVQLFLRQLREKGVAEE
                     HIARLHAPVGYNIGAETPQEIAISVLAEILQVKNNAPGGLMMKPSHPSGHQLVVIRGA
                     GDIASGVALRLYHAGFKVIMLEVEKPTVIRCTVAFAQAVFDGEMTVEGVTARLATSSA
                     EAMKLTERGFIPVMVDPTCSLLDELKPLCVVDAILAKQNLGTRADMAPVTIALGPGFT
                     AGKDCHAVIETNRGHWLGQVIYSGCAQENTGVPGNIMGHTTRRVIRAPAAGIMRSNVK
                     LGDLVKEGDVIAWIGEHEIKAPLTGMVRGLLNDGLAVVGGFKIGDIDPRGETADFTSV
                     SDKARAIGGGVLEALMMLMHQGVKATKEVLEVA"
     gene            complement(8142..8912)
                     /gene="yqeC"
                     /locus_tag="J1Y83_000007"
     CDS             complement(8142..8912)
                     /gene="yqeC"
                     /locus_tag="J1Y83_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417352.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative selenium-dependent hydroxylase
                     accessory protein YqeC"
                     /protein_id="HBA7628204.1"
                     /translation="MKSIIDPSALFIDLGAQKRPTVISIVGAGGKTSLLFWLAELFQA
                     SGRRVLITTTTHMFMPTSHWPVVFCRDPAMLPHASFISPISFCFHCWKANQGKVQGFT
                     PEAIDALVQRPECDVILIEADGSRGMPLKAPDEHEPCIPKSSCCVIAVMGGHILGAKV
                     STENVHRWSQFADITGLTPDAPLQLSDLVALVRHPQGAFKNVPQGCRRVWFINRFSQC
                     ENAIAQSELLQPLQQHNVEAIWLGDIQEHPAIARRFVN"
     gene            9016..9594
                     /gene="mocA"
                     /locus_tag="J1Y83_000008"
     CDS             9016..9594
                     /gene="mocA"
                     /locus_tag="J1Y83_000008"
                     /EC_number="2.7.7.76"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor cytidylyltransferase"
                     /protein_id="HBA7628205.1"
                     /translation="MSAIDCIITAAGLSSRMGQWKMMLPWQQGTILDTSIKNALQFCS
                     RIILVTGYRGNELHERYANQSNITIIHNPDYAQGLLTSVKAAVPAVQTEHCFLTHGDM
                     PTLTIDIFRKIWSLRNDGAILPLHNGIPGHPILVSKPCLMQAIQRPNVTNMRQALLMG
                     EHYSVEIENAEIILDIDTPDDFITAKKRYTEI"
     gene            9916..13014
                     /gene="ygfK"
                     /locus_tag="J1Y83_000009"
     CDS             9916..13014
                     /gene="ygfK"
                     /locus_tag="J1Y83_000009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative selenate reductase subunit YgfK"
                     /protein_id="HBA7628206.1"
                     /translation="MGDIMRPIPFEELLTRIFDEYQQQRSIFGIPEQQFYSPVKGKTV
                     SVFGETCATPVGPAAGPHTQLAQNIVTSWLTGGRFIELKTVQILDRLELEKPCIDAED
                     ECFNTEWSTEFTLLKAWDEYLKAWFALHLLEAMFQPSDSGKSFIFNMSVGYNLEGIKQ
                     PPMQQFIDNMMDASDHPKFAQYRDALNKLLQDEAFLARHGLQEKRESLQALPARIPTS
                     MVQGVTLSTMHGCPPHEIEAICRYMLEEKGLNTFVKLNPTLLGYARVREILDVCGFGY
                     IGLKEESFDHDLKLTQALEMLERLMALAKEKSLGFGVKLTNTLGTINNKGALPGEEMY
                     MSGRALFPLSINVAAVLSRAFDGKLPISYSGGASQLTIRDIFDTGIRPITMATDLLKP
                     GGYLRLSACMRELEGSDAWELNHVDVERLNRLAADALTMEYTQKHWKPEERIEVAEDL
                     PLTDCYVAPCVTACAIKQDIPEYIRLLGEHRYADALELIYQRNALPAITGHICDHQCQ
                     YNCTRLDYDSALNIRELKKVALEKGWDEYKQRWHKPAGSGSRHPVAVIGAGPAGLAAG
                     YFLARAGHPVTLFEREANAGGVVKNIIPQFRIPAELIQHDIDFVAAHGVKFEYGCSPD
                     LTVEQLKNQGFHYVLIATGTDKNSGVKLAGDNQNVWKSLPFLREYNKGTALKLGKHVV
                     VVGAGNTAMDCARAALRVPGVEKATVVYRRSLQEMPAWREEYEEALHDGVEFRFLNNP
                     ERFDADGTLTLRVMSLGEPDEKGRRRPVETNETVTLHVDSLITAIGEQQDTEALNAMG
                     VPLDKNGWPDVDHNGETRLTDVFMIGDVQRGPSSIVAAVGTARRATDAILSRENIRSH
                     QNDKYWNNVNPAEIYQRKGDISVTLVNSDDRDAFVAQEAARCLECNYVCSKCVDVCPN
                     RANVSIAVPGFQNRFQTLHLDAYCNECGNCAQFCPWNGKPYKDKITVFSLSQDFDNSS
                     NPGFLVEDCRVRVRLNNQSWVLNIDSEGQFNNVPPELNDMCRIISHVHQHHHYLLGRV
                     EV"
     gene            13017..14345
                     /gene="ssnA"
                     /locus_tag="J1Y83_000010"
     CDS             13017..14345
                     /gene="ssnA"
                     /locus_tag="J1Y83_000010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative aminohydrolase SsnA"
                     /protein_id="HBA7628207.1"
                     /translation="MLILKNVTAVQLHPAKVQEGVDIAIENDVIVAIGDALTQRYPDA
                     SYKEMHGRIVMPGIVCSHNHFYSGLSRGIMANIAPCPDFISTLKNLWWRLDRALDEES
                     LYYSGLICSLEAIKSGCTSVIDHHASPAYIGGSLSTLRDAFLKVGLRAMTCFETTDRN
                     NGIKELQEGVEENIRFARQIDEAKKAATEPYLVEAHIGAHAPFTVPDAGLEMLREAVK
                     STGRGLHIHAAEDLYDVSYSHHWYGKDLLARLAQFDLIDSKTLVAHGLYLSKDDITLL
                     NQRDAFLVHNARSNMNNHVGYNHHLSDIRNLALGTDGIGSDMFEEMKFAFFKHRDAGG
                     PLWPDSFAKALANGNELMSRNFGAKFGLLEAGYKADLTICDYNSPTPLLADNIAGHIA
                     FGMGSGSVHSVMVNGVMVYEERQFNFDCDSIYAQARKAAASMWRRMDALA"
     gene            14396..15175
                     /locus_tag="J1Y83_000011"
     CDS             14396..15175
                     /locus_tag="J1Y83_000011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase
                     FAD-binding subunit"
                     /protein_id="HBA7628208.1"
                     /translation="MIEQFFRPDSVEQALELKRRYQDEAVWFAGGSKLNATPTRTDKK
                     IAISLQDLELDWVDWDNGALRIGAMSRLQPLRDARFIPAALREALGFVYSRHIRNQST
                     IGGEIAARQEESVLLPVLLALDAELVFGNGETLSIEDYLACPCDRLLTEIIIKDPYRT
                     CATRKISRSQAGLTVVTAAVAITDHDGMRIALDGVASKALRLHDVETQNLEGNALEQA
                     VANAIFPQEDLRGSVAYKRYITGVLVADLYDDCQQAGEEAV"
     gene            15172..18042
                     /locus_tag="J1Y83_000012"
     CDS             15172..18042
                     /locus_tag="J1Y83_000012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase
                     Mo/Fe-S-binding subunit"
                     /protein_id="HBA7628209.1"
                     /translation="MIIHFTLNGAPQELTVNPGENVQKLLFNMGMHSVRNSDDGFGFA
                     GSDAIIFNGNIVNASLLIAAQLEKADIRTAESLGKWNELSLVQQAMVDVGVVQSGYND
                     PAAALIITDLLDRIDAPTREEIDDALSGLFSRDAGWQQYYQVIELAVARKNNPQATID
                     IAPTFRDDLEVIGKHYPKTDAAKMVQAKPCYVEDRVTADACVIKMLRSPHAHALITHL
                     DVSKAEALPGVVHVITHLNCPDIYYTPGGQSAPEPSPLDRRMFGKKMRHVGDRVAAVV
                     AESEDIALEALKLIDVEYEVLKPVMSIDEAMAEDAPVVHDEPVVYVAGAPDTLEDDNS
                     HAAQRGEHMIINFPIGSRPRKNIAASIHGHIGDMDKGFADADVIIERTYNSTQAQQCP
                     TETHICFTRMDGDRLVIHASTQVPWHLRRQVARLVGMKQHKVHVIKERVGGGFGSKQD
                     ILLEEVCAWATCVTGRPVLFRYTREEEFIANTSRHVAKVTVKLGAKKDGRLTAVKMDF
                     RANTGPYGNHSLTVPCNGPALSLPLYPCDNVDFQVTTYYSNICPNGAYQGYGAPKGNF
                     AITMALAELAEQLQIDQLEIIERNRVHEGQELKILGAIGEGKAPTSVPSAASCALEEI
                     LRQGREMIQWSSPKPQNGDWHIGRGVAIIMQKSGIPDIDQANCMIKLESDGTFIVHSG
                     GADIGTGLDTVVTKLAAEVLHCPPQDVHVISGDTDHALFDKGAYASSGTCFSGNAARL
                     AAENLREKILFHGAQILGEPVADVQLATPGVVRGKKGEVSFGEIAHKGETGTGFGSLV
                     GTGSYITPDFAFPYGANFAEVAVNTRTGEIRLDKFYALLDCGTPVNPELALGQIYGAT
                     LRAIGHSMSEEIIYDAEGHPLTRDLRSYGAPKIGDIPRDFRAVLVPSDDKVGPFGAKS
                     ISEIGVNGAAPAIATAIHDACGIWLREWHFTPEKILTALEKI"
     gene            18207..19607
                     /gene="xanQ"
                     /locus_tag="J1Y83_000013"
     CDS             18207..19607
                     /gene="xanQ"
                     /locus_tag="J1Y83_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417358.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine/proton symporter XanQ"
                     /protein_id="HBA7628210.1"
                     /translation="MSDINHAGSDLIFELEDRPPFHQALVGAITHLLAIFVPMVTPAL
                     IVGAALQLSAETTAYLVSMAMIASGIGTWLQVNRYGIVGSGLLSIQSVNFSFVTVMIA
                     LGSSMKSDGFHEELIMSSLLGVSFVGAFLVVGSSFILPYLRRVITPTVSGIVVLMIGL
                     SLIKVGIIDFGGGFAAKSSGTFGNYEHLGVGLLVLIVVIGFNCCRSPLLRMGGIAIGL
                     CVGYIASLCLGMVDFSSMRNLPLITIPHPFKYGFSFSFHQFLVVGTIYLLSVLEAVGD
                     ITATAMVSRRPIQGEEYQSRLKGGVLADGLVSVIASAVGSLPLTTFAQNNGVIQMTGV
                     ASRYVGRTIAVMLVILGLFPMIGGFFTTIPSAVLGGAMTLMFSMIAIAGIRIIITNGL
                     KRRETLIVATSLGLGLGVSYDPEIFKILPASIYVLVENPICAGGLTAILLNIILPGGY
                     RQENVLPGITSAEEMD"
     gene            19625..20941
                     /gene="guaD"
                     /locus_tag="J1Y83_000014"
     CDS             19625..20941
                     /gene="guaD"
                     /locus_tag="J1Y83_000014"
                     /EC_number="3.5.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311783.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanine deaminase"
                     /protein_id="HBA7628211.1"
                     /translation="MSGEHTLKAVRGSFIDVTRTVDNPEEIASALRFIEDGLLLIKQG
                     KVEWFGEWEDGKHQIPDTIRVRDYRGKLIVPGFVDTHIHYPQSEMVGAYGEQLLEWLN
                     KHTFPTERRYEDLEYAREMSAFFIKQLLRNGTTTALVFGTVHPQSVDALFEAASHINM
                     RMIAGKVMMDRNAPDYLLDTAESSYHQSKELIERWHKNGRLLYAITPRFAPTSSPEQM
                     AMAQRLKEEYPDTWVHTHLCENKDEIAWVKSLYPDHDGYLDVYHQYGLTGKNCVFAHC
                     VHLEEKEWDRLSETKSSIAFCPTSNLYLGSGLFNLKKAWQKKVKVGMGTDIGAGTTFN
                     MLQTLNEAYKVLQLQGYRLSAYEAFYLATLGGAKSLGLDDLIGNFLPGKEADFVVMEP
                     TATPLQQLRYDNSVSLVDKLFVMMTLGDDRSIYRTYVDGRLVYERN"
     gene            20977..22344
                     /locus_tag="J1Y83_000015"
     CDS             20977..22344
                     /locus_tag="J1Y83_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HBA7628212.1"
                     /translation="MSGDILQTPDAPKPQGALDNYFKITARGSTVRQEVLAGLTTFLA
                     MVYSVIVVPGMLGKAGFPPAAVFVATCLVAGFGSLLMGLWANLPMAIGCAISLTAFTA
                     FSLVLGQQISVPVALGAVFLMGVIFTAISVTGVRTWILRNLPMGIAHGTGIGIGLFLL
                     LIAANGVGMVIKNPIEGLPVALGAFTSFPVMMSLLGLAVIFGLEKCRVPGGILLVIIA
                     ISIIGLIFDPAVKYHGLVAMPSLTGEDGKSLIFSLDIMGALQPTVLPSVLALVMTAVF
                     DATGTIRAVAGQANLLDKDNQIINGGKALTSDSVSSIFSGLVGAAPAAVYIESAAGTA
                     AGGKTGLTATVVGVLFLMILFLSPLSFLIPGYATAPALMYVGLLMLSNVSKLDFNDFI
                     DAMAGLVCAVFIVLTCNIVTGIMLGFVTLVVGRVFAREWQKLNIGTVIITAALVAFYA
                     GGWAI"
     gene            complement(22380..22868)
                     /locus_tag="J1Y83_000016"
     CDS             complement(22380..22868)
                     /locus_tag="J1Y83_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417362.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HBA7628213.1"
                     /translation="MKSLIIVNPADCIGCRTCEVACVVAHPSEQELNADIFLPRLKVQ
                     RLDSISAPVMCHQCENAPCVGACPVGALTMGEQVVQANSARCIGCQSCVSACPFGMIT
                     IQSLPGDTRQQIVKCDLCEQREEGPACVESCPTQALQLLTERELRRIRQQRIAASGAN
                     PL"
     gene            complement(22868..24787)
                     /gene="ygfT"
                     /locus_tag="J1Y83_000017"
     CDS             complement(22868..24787)
                     /gene="ygfT"
                     /locus_tag="J1Y83_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent uric acid utilization protein
                     YgfT"
                     /protein_id="HBA7628214.1"
                     /translation="MNKFIAAEAAECIGCHACEIACAVAHNQENWPLSHSDFRPRIHV
                     VGKGQAANPVACHHCNNAPCVTACPVNALTFQSDSVQLDEQKCIGCKRCAIACPFGVV
                     EMVDTIAQKCDLCNQRSSGTQACIDVCPTQALRLMDDKGLQQIKVARQRKTAAGKASS
                     DAQPSRSAALLPVNSRKGADKISASERKTHFGEIYCGLDPQQATYESDRCVYCAEKAN
                     CNWHCPLHNAIPDYIRLVQEGKIIEAAELCHQTSSLPEICGRVCPQDRLCEGACTLKD
                     HSGAVSIGNLERYITDTALAMGWRPDVSKVVPRIEKVAVIGAGPAGLGCADILARAGV
                     QVDVFDRHPEIGGMLTFGIPPFKLDKTVLSQRREIFTAMGIDFHLNCEIGRDISFNEL
                     TAEYDAVFLGVGTYGMMRADLPHEDAPGVIQALPFLTAHTRQLMGLPESAEYPLTDVE
                     GKRVVVLGGGDTTMDCLRTSIRLNAASVTCAYRRDEVSMPGSRKEVVNAREEGVEFQF
                     NVQPQYIACDEDGRLTAVGLIRTAMGEPGPDGRRRPRPVAGSEFELPADVLIMAFGFQ
                     AHTMPWLQGSGIKLDKWGLIQTGDVGYLPTQTHLKKVFAGGDAVHGADLVVTAMAAGR
                     QAARDMLTLFDTKAS"
     gene            25223..26671
                     /locus_tag="J1Y83_000018"
     CDS             25223..26671
                     /locus_tag="J1Y83_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417364.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine permease"
                     /protein_id="HBA7628215.1"
                     /translation="MSAIDSQLPSSSGQDRPTDEVDRILSPGKLIILGLQHVLVMYAG
                     AVAVPLMIGDRLGLSKEAIAMLISSDLFCCGIVTLLQCIGIGRFMGIRLPVIMSVTFA
                     AVTPMIAIGMNPDIGLLGIFGATIAAGFITTLLAPLIGRLMPLFPPLVTGVVITSIGL
                     SIIQVGIDWAAGGKGNPQYGNPVYLGISFAVLIFILLITRYAKGFMSNVAVLLGIVFG
                     FLLSWMMNEVNLSGLHDASWFAIVTPMSFGMPIFDPVSILTMTAVLIIVFIESMGMFL
                     ALGEIVGRKLSSHDIIRGLRVDGVGTMIGGTFNSFPHTSFSQNVGLVSVTRVHSRWVC
                     ISSGIILILFGMVPKMAVLVASIPQFVLGGAGLVMFGMVLATGIRILSRCNYTTNRYN
                     LYIVAISLGVGMTPTLSHDFFSKLPAVLQPLLHSGIMLATLSAVVLNVFFNGYQHHAD
                     LVKESVSDKDLKVRTVRMWLLMRKLKKNEHGE"
     gene            26673..26798
                     /locus_tag="J1Y83_000019"
     CDS             26673..26798
                     /locus_tag="J1Y83_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA7628216.1"
                     /translation="MNLLMRAIFSLLLLFTLSIPVISDCVAMAIESRFKYMMLLF"
     gene            complement(26795..26866)
                     /locus_tag="J1Y83_000020"
     CDS             complement(26795..26866)
                     /locus_tag="J1Y83_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA7628217.1"
                     /translation="MINQVSVYRQPPVLSGCRQVKTI"
     gene            26921..27469
                     /gene="idi"
                     /locus_tag="J1Y83_000021"
     CDS             26921..27469
                     /gene="idi"
                     /locus_tag="J1Y83_000021"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HBA7628218.1"
                     /translation="MQTEHVILLNAQGVPTGTLEKYAAHTADTLLHLAFSSWLFNAKG
                     QLLVTRRALSKKAWPGVWTNSVCGHPQPGESNEDAVIRRCRYELGVEITPPESIYPDF
                     RYRATDPNGIVENEVCPVFAARTTSALQINDDEVMDYQWCDLADVLRGIDATPWAFSP
                     WMVMQAANSEARKLLSAFAQHN"
     gene            complement(27512..29029)
                     /gene="lysS"
                     /locus_tag="J1Y83_000022"
     CDS             complement(27512..29029)
                     /gene="lysS"
                     /locus_tag="J1Y83_000022"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HBA7628219.1"
                     /translation="MSEQHAQGADAVVDLNNELKTRREKLANLREQGIAFPNDFRRDH
                     TSDQLHAEFDGKENEELEALNIEVAVAGRMMTRRIMGKASFVTLQDVGGRIQLYVARD
                     DLPEGVYNEQFKKWDLGDILGAKGKLFKTKTGELSIHCTELRLLTKALRPLPDKFHGL
                     QDQEARYRQRYLDLISNDESRNTFKVRSQILSGIRQFMVNRGFMEVETPMMQVIPGGA
                     AARPFITHHNALDLDMYLRIAPELYLKRLVVGGFERVFEINRNFRNEGISVRHNPEFT
                     MMELYMAYADYKDLIELTESLFRTLAQDILGKTEVTYGDVTLDFGKPFEKLTMREAIK
                     KYRPETDMADLDNFDSAKAIAESIGIHVEKSWGLGRIVTEIFEEVAEAHLIQPTFITE
                     YPAEVSPLARRNDVNPEITDRFEFFIGGREIGNGFSELNDAEDQAQRFLDQVAAKDAG
                     DDEAMFYDEDYVTALEHGLPPTAGLGIGIDRMVMLFTNSHTIRDVILFPAMRPVK"
     gene            complement(29039..30137)
                     /gene="prfB"
                     /locus_tag="J1Y83_000023"
     CDS             complement(join(29039..30061,30063..30137))
                     /gene="prfB"
                     /locus_tag="J1Y83_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311790.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HBA7628220.1"
                     /translation="MFEINPVNNRIQDLTERSDVLRGYLDYDAKKERLEEVNAELEQP
                     DVWNEPERAQALGKERSSLEAVVDTLDQMKQGLEDVSGLLELAVEADDEETFNEAVAE
                     LDALEEKLAQLEFRRMFSGEYDSADCYLDIQAGSGGTEAQDWASMLERMYLRWAESRG
                     FKTEIIEESEGEVAGIKSVTIKISGDYAYGWLRTETGVHRLVRKSPFDSGGRRHTSFS
                     SAFVYPEVDDDIDIEINPADLRIDVYRASGAGGQHVNRTESAVRITHIPTGIVTQCQN
                     DRSQHKNKDQAMKQMKAKLYELEMQKKNAEKQAMEDNKSDIGWGSQIRSYVLDDSRIK
                     DLRTGVETRNTQAVLDGSLDQFIEASLKAGL"
     gene            complement(30228..31961)
                     /gene="recJ"
                     /locus_tag="J1Y83_000024"
     CDS             complement(30228..31961)
                     /gene="recJ"
                     /locus_tag="J1Y83_000024"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HBA7628221.1"
                     /translation="MKQQIQLRRREVDETADLPAELPPLLRRLYASRGVRSAQELERS
                     VKGMLPWQQLSGVEKAVEILYNAFREGTRIIVVGDFDADGATSTALSVLAMRSLGCSN
                     IDYLVPNRFEDGYGLSPEVVDQAHARGAQLIVTVDNGISSHAGVEHARSLGIPVIVTD
                     HHLPGDTLPAAEAIINPNLRDCNFPSKSLAGVGVAFYLMLALRTFLRDQGWFDERGIA
                     IPNLAELLDLVALGTVADVVPLDANNRILTWQGMSRIRAGKCRPGIKALLEVANRDAQ
                     KLAASDLGFALGPRLNAAGRLDDMSVGVALLLCDNIGEARVLANELDALNQTRKEIEQ
                     GMQVEALTLCEKLERSRDTLPGGLAMYHPEWHQGVVGILASRIKERFHRPVIAFAPAG
                     DGTLKGSGRSIQGLHMRDALERLDTLYPGMMLKFGGHAMAAGLSLEEDKFELFQQRFG
                     ELVTEWLDPSLLQGEVISDGPLSAAEMTMEVAQLLRDAGPWGQMFPEPLFDGHFRLLQ
                     QRLVGERHLKVMVEPVGGGPLLDGIAFNVDTALWPDNGVREVQLAYKLDINEFRGNRS
                     LQIIIDNIWPI"
     gene            complement(31967..32677)
                     /gene="dsbC"
                     /locus_tag="J1Y83_000025"
     CDS             complement(31967..32677)
                     /gene="dsbC"
                     /locus_tag="J1Y83_000025"
                     /EC_number="5.3.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional protein-disulfide
                     isomerase/oxidoreductase DsbC"
                     /protein_id="HBA7628222.1"
                     /translation="MKKGFMLFTLLAAFSGFVQADDAAIQQTLAKMGIKSSDIQPAPV
                     AGMKTVLTNSGVLYITDDGKHIIQGPMYDVSGTAPVNVTNKMLLKQLNALEKEMIVYK
                     APQEKHVITVFTDITCGYCHKLHEQMADYNALGITVRYLAFPRQGLDSDAEKEMKAIW
                     CAKDKNKAFDDVMAGKSVAPASCDVDIADHYALGVQLGVSGTPAVVLSNGTLVPGYQP
                     PKDMKEFLDEHQKMTSGK"
     gene            complement(32702..33598)
                     /gene="xerD"
                     /locus_tag="J1Y83_000026"
     CDS             complement(32702..33598)
                     /gene="xerD"
                     /locus_tag="J1Y83_000026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HBA7628223.1"
                     /translation="MKQDLARIEQFLDALWLEKNLAENTLNAYRRDLSMMVEWLHHRG
                     LTLATAQSDDLQALLAERLEGGYKATSSARLLSAVRRLFQYLYREKFREDDPSAHLAS
                     PKLPQRLPKDLSEAQVERLLQAPLIDQPLELRDKAMLEVLYATGLRVSELVGLTMSDI
                     SLRQGVVRVIGKGNKERLVPLGEEAVYWLETYLEHGRPWLLNGVSIDVLFPSQRAQQM
                     TRQTFWHRIKHYAVLAGIDSEKLSPHVLRHAFATHLLNHGADLRVVQMLLGHSDLSTT
                     QIYTHVATERLRQLHQQHHPRA"
     gene            33710..34231
                     /gene="fldB"
                     /locus_tag="J1Y83_000027"
     CDS             33710..34231
                     /gene="fldB"
                     /locus_tag="J1Y83_000027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin FldB"
                     /protein_id="HBA7628224.1"
                     /translation="MNMGLFYGSSTCYTEMAAEKIRDIIGPELVTLHNLKDDSPKLME
                     QYDVLILGIPTWDFGEIQEDWEAVWDQLDDLNLEGKIVALYGLGDQLGYGEWFLDALG
                     MLHDKLSTKGVKFVGYWPTEGYEFTSPKPVIADGQLFVGLALDETNQYDLSDERIQSW
                     CEQILNEMAEHYA"
     gene            complement(34271..34678)
                     /locus_tag="J1Y83_000028"
     CDS             complement(34271..34678)
                     /locus_tag="J1Y83_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA7628225.1"
                     /translation="MVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMV
                     LLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQEWSIVKAPWMIKSGMMLRLRSD
                     SGKRQHLWLAADSMDEAEWRDLRRILLQQETQR"
     gene            complement(34659..34925)
                     /gene="sdhE"
                     /locus_tag="J1Y83_000029"
     CDS             complement(34659..34925)
                     /gene="sdhE"
                     /locus_tag="J1Y83_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000354044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD assembly factor SdhE"
                     /protein_id="HBA7628226.1"
                     /translation="MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFI
                     RLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRNRERGPVAI"
     gene            35168..36148
                     /gene="ygfZ"
                     /locus_tag="J1Y83_000030"
     CDS             35168..36148
                     /gene="ygfZ"
                     /locus_tag="J1Y83_000030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-modifying protein YgfZ"
                     /protein_id="HBA7628227.1"
                     /translation="MAFTPFPPRQPTASARLPLTLMTLDDWALATITGADSEKYMQGQ
                     VTADVSQMTEDQHLLAAHCDAKGKMWSNLRLFRDGDGFAWIERRSVREPQLTELKKYA
                     VFSKVTIAPDDERVLLGVAGFQARAALANLFSELPSREKQVVKEGATTLLWFEHPAER
                     FLIVTDEATANMLTDKLRGEAELNNSQQWLALNIEAGFPVIDAANSGQFIPQATNLQA
                     LGGISFKKGCYTGQEMVARAKFRGANKRALWLLAGSASRLPEAGEDLELKMGENWRRT
                     GTVLAAVKLEDGQVVVQVVMNNDMEPDSIFRVRDDANTLHIEPLPYSLEE"
     gene            complement(36225..36884)
                     /locus_tag="J1Y83_000031"
     CDS             complement(36225..36884)
                     /locus_tag="J1Y83_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HBA7628228.1"
                     /translation="MVQKPLIKQGYSLAEEIANSVSHGIGLVFGIVGLVLLLVQAVDL
                     NASATAITSYSLYGGSMILLFLASTLYHAIPHQRAKMWLKKFDHCAIYLLIAGTYTPF
                     LLVGLDSPLARGLMIVIWSLALLGILFKLTIAHRFKILSLVTYLAMGWLSLVVIYEMA
                     VKLAAGSVTLLAVGGVVYSLGVIFYVCKRIPYNHAIWHGFVLGGSVCHFLAIYLYIGQ
                     A"
     gene            complement(37048..37359)
                     /locus_tag="J1Y83_000032"
     CDS             complement(37048..37359)
                     /locus_tag="J1Y83_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HBA7628229.1"
                     /translation="MQPNDITFFQRFQDDILAGRKTITIRDESESHFKTGDVLRVGRF
                     EDDGYFCTIEVTATSTVTLDTLTEKHAEQENMTLTELKKVIADIYPDQTQFYVIEFKC
                     L"
     gene            37404..38837
                     /locus_tag="J1Y83_000033"
     CDS             37404..38837
                     /locus_tag="J1Y83_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HBA7628230.1"
                     /translation="MKKLTLPKDFLWGGAVAAHQVEGGWNKGGKGPSICDVLTGGAHG
                     VPREITKEVVPGKYYPNHEAVDFYGHYKEDIKLFAEMGFKCFRTSIAWTRIFPKGDEA
                     QPNEEGLKFYDDMIDELLKYNIEPVITLSHFEMPLHLVQQYGSWTNRKVVDFFVRFAE
                     VVFERYKHKVKYWMTFNEINNQRNWRAPLFGYCCSGVVYTEHENPEETMYQVLHHQFV
                     ASALAVKAAHRINPEMKVGCMLAMVPLYPYSCNPDDVMFAQESMRERYVFTDVQLRGY
                     YPSYVLNEWERRGFNIKMEDGDLDVLREGTCDYLGFSYYMTNAVKAEGGTGDAISGFE
                     GSVPNPYVKASDWGWQIDPVGLRYALCELYERYQKPLFIVENGFGAYDKVEDDGSIND
                     DYRIDYLRAHIEEMKKAVTYDGVDLMGYTPWGCIDCVSFTTGQYSKRYGFIYVNKHDD
                     GTGDMSRSRKKSFDWYKEVIASNGENL"
     gene            complement(39003..41876)
                     /gene="gcvP"
                     /locus_tag="J1Y83_000034"
     CDS             complement(39003..41876)
                     /gene="gcvP"
                     /locus_tag="J1Y83_000034"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase"
                     /protein_id="HBA7628231.1"
                     /translation="MTQTLSQLENSGAFIERHIGPDAAQQQEMLNAVGAQSLNALTGQ
                     IVPKDIQLATPPQVGAPATEYAALAELKAIASRNKRFTSYIGMGYTAVQLPPVILRNM
                     LENPGWYTAYTPYQPEVSQGRLEALLNFQQVTLDLTGLDMASASLLDEATAAAEAMAM
                     AKRVSKLKNANRFFVASDVHPQTLDVVRTRAETFGFEVIVDDAQKVLDHQDVFGVLLQ
                     QVGTTGEIHDYTALISELKSRKIVVSVAADIMALVLLTAPGKQGADIVFGSAQRFGVP
                     MGYGGPHAAFFAAKDEYKRSMPGRIIGVSKDAAGNTALRMAMQTREQHIRREKANSNI
                     CTSQVLLANIASLYAVYHGPVGLKRIANRIHRLTDILAAGLQQKGLKLRHAHYFDTLC
                     VEVVDKAGVLARAEAAEINLRSDILNAVGITLDETTTRENVMQLFSVLLGDNHGLDID
                     TLDKDVAHDSRSIQAAMLRDDEILTHPVFNRYHSETEMMRYMHSLERKDLALNQAMIP
                     LGSCTMKLNAAAEMIPITWPEFAELHPFCPPEQAEGYQQMIAQLADWLVKLTGYDAVC
                     MQPNSGAQGEYAGLLAIRHYHESRNEGHRDICLIPASAHGTNPASAHMAGMQVVVVAC
                     DKNGNIDLTDLRAKAEQAGDNLSCIMVTYPSTHGVYEETIREVCEVVHQFGGQVYLDG
                     ANMNAQVGITSPGFIGADVSHLNLHKTFCIPHGGGGPGMGPIGVKAHLAPFVPGHSVV
                     QIEGMLTRQGAVSAAPFGSASILPISWMYIRMMGAEGLKKASQVAILNANYIASRLQD
                     AFPVLYTGRDGRVAHECILDIRPLKEETGISELDIAKRLIDYGFHAPTMSFPVAGTLM
                     VEPTESESKVELDRFIDAMLAIRAEIDQVKAGVWPLEDNPLVNAPHIQSELVAEWAHP
                     YSREVAVFPAGVADKYWPTVKRLDDVYGDRNLFCSCVPISEYQ"
     gene            complement(41994..42383)
                     /gene="gcvH"
                     /locus_tag="J1Y83_000035"
     CDS             complement(41994..42383)
                     /gene="gcvH"
                     /locus_tag="J1Y83_000035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HBA7628232.1"
                     /translation="MSNIPAELKYSKEHEWLRKEADGTYTVGITEHAQELLGDMVFVD
                     LPEVGATVSAGDDCAVAESVKAASDIYAPVSGEIVAVNDALSDSPELVNSEPYAGGWI
                     FKIKASDESELESLLDATAYEALLEDE"
     gene            complement(42407..43501)
                     /gene="gcvT"
                     /locus_tag="J1Y83_000036"
     CDS             complement(42407..43501)
                     /gene="gcvT"
                     /locus_tag="J1Y83_000036"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HBA7628233.1"
                     /translation="MAQQTPLYEQHTLCGARMVDFHGWMMPLHYGSQIDEHHAVRTEA
                     GMFDVSHMTIVDLRGSRTREFLRYLLANDVAKLTKSGKALYSGMLNASGGVIDDLIVY
                     YFTEDFFRLVVNSATREKDLSWITQHAEPFGIEITVRDDLSMIAVQGPNAQAKAATLF
                     NDAQRQAVEGMKPFFGVQAGDLFIATTGYTGEAGYEIALPNEKAADFWRALVEAGVKP
                     CGLGARDTLRLEAGMNLYSQEMDETISPLAANMGWTIAWEPADRDFIGREALEAQREQ
                     GTEKLVGLVMTEKGVLRNELPVRFTDAQGNQHEGIITSGTFSPTLGYSIALARVPEGI
                     GETAIVQIRNREMPVKVTKPVFVRNGKAVA"
     gene            43792..43875
                     /locus_tag="J1Y83_000037"
     CDS             43792..43875
                     /locus_tag="J1Y83_000037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA7628234.1"
                     /translation="MSKNTKSKIHNIRKYNAKTEVKLVYFK"
     gene            complement(43949..45151)
                     /gene="ubiI"
                     /locus_tag="J1Y83_000038"
                     /gene_synonym="visC"
     CDS             complement(43949..45151)
                     /gene="ubiI"
                     /locus_tag="J1Y83_000038"
                     /gene_synonym="visC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent 2-octaprenylphenol hydroxylase"
                     /protein_id="HBA7628235.1"
                     /translation="MQSVDVAIVGGGMVGLAVACGLQGSGLRVAVLEQRVPEPLAADA
                     PPQLRVSAINAASEKLLTRLGVWQDILSRRASCYHGMEVWDKDSFGHISFDDQSMGYS
                     HLGHIVENSVIHYALWNKAQQSSDINLLAPAELQQVAWGENETFLTLKDGSMLTARLV
                     IGADGANSWLRNKADIPLTFWDYQHHALVATIRTEEPHDAVARQVFHGEGILAFLPLS
                     DPHLCSIVWSLSPEEAQRMQQASEDEFNRALNIAFDNRLGLCKVESARQVFPLTGRYA
                     RQFAAHRLALVGDAAHTIHPLAGQGVNLGFMDAAELIAELKRLHRQGKDIGQYIYLRR
                     YERSRKHSAALMLAGMQGFRDLFSGTNPAKKLLRDIGLKLADTLPGVKPQLIRQAMGL
                     NDLPEWLR"
     gene            complement(45174..46352)
                     /gene="ubiH"
                     /locus_tag="J1Y83_000039"
                     /gene_synonym="visB"
     CDS             complement(45174..46352)
                     /gene="ubiH"
                     /locus_tag="J1Y83_000039"
                     /gene_synonym="visB"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-octaprenyl-6-methoxyphenyl hydroxylase"
                     /protein_id="HBA7628236.1"
                     /translation="MSVIIVGGGMAGATLALAISRLSHGALSVHLIEATAPESHAHPG
                     FDGRAIALAAGTCQQLARIGVWQSLADCATAITTVHVSDRGHAGFVTLAAEDYQLAAL
                     GQVVELHNVGQRLFALLRKAPGVTLHCPDRVANVARTQSHVEVTLESGETLTGRVLVA
                     ADGTHSALATVCGVDWQQEPYEQLAVIANVATSVAHEGRAFERFTKHGPLAMLPMSDG
                     RCSLVWCHPLERREEVLSWSDEKFCRELQSAFGWRLGQITHAGKRSAYPLALTRAARP
                     ITHRTVLVGNAAQTLHPIAGQGFNLGMRDVMSLAETLTQAQERGEDMGDYGVLCRYQQ
                     RRQSDREATIGVTDSLVHLFANRWTPLVVGRNIGLMTMELFTPARDVLAQRTLGWVAR
                     "
     gene            complement(46349..47674)
                     /gene="pepP"
                     /locus_tag="J1Y83_000040"
     CDS             complement(46349..47674)
                     /gene="pepP"
                     /locus_tag="J1Y83_000040"
                     /EC_number="3.4.11.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro aminopeptidase"
                     /protein_id="HBA7628237.1"
                     /translation="MSDISRQEFQRRRQALVEQMQPGSAALIFAAPEVTRSADSEYPY
                     RQNSDFWYFTGFNEPEAVLVLIKSDDTHNHSVLFNRVRDLTAEIWFGRRLGQDAAPEK
                     LGVDRALAFSEINQQLYQLLNGLDVVYHAQGEHAYADEIVNSALEKLRKGSRQNLTAP
                     ATMIDWRPVVHEMRLFKSPEEIAVLRRAGEITALAHTRAMEKCRPGMFEYHLEGEIHH
                     EFNRHGARYPSYNTIVGSGENGCILHYTENECELRDGDLVLIDAGCEYKGYAGDITRT
                     FPVNGKFTQAQREIYDIVLESLETSLRLYRPGTSIQEVTGEVVRIMVSGLVKLGILKG
                     DVDELIAQNAHRPFFMHGLSHWLGLDVHDVGVYGQDRSRILEPGMVLTVEPGLYIAPD
                     AEVPEQYRGIGIRIEDDIVITETGNENLTASVVKKPEEIEALMAAARKQ"
     gene            complement(47700..48278)
                     /gene="ygfB"
                     /locus_tag="J1Y83_000041"
     CDS             complement(47700..48278)
                     /gene="ygfB"
                     /locus_tag="J1Y83_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein YgfB"
                     /protein_id="HBA7628238.1"
                     /translation="MSIQNEMPGYNEMNQYLNQQGTGLTPAEMHGLISGIICGGNDDS
                     SWLPLLHDLTNEGMAFGHELAQALRKMHSATSDALQDDGFLFQLYLPDGDDVSVFDRA
                     DALAGWVNHFLLGLGVTQPKLDKVTGETGEAIDDLRNIAQLGYDEDEDQEELEMSLEE
                     IIEYVRVAALLCHDTFTHPQPTAPEVQKPTLH"
     gene            48446..48775
                     /gene="zapA"
                     /locus_tag="J1Y83_000042"
     CDS             48446..48775
                     /gene="zapA"
                     /locus_tag="J1Y83_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HBA7628239.1"
                     /translation="MSAQPVDIQIFGRSLRVNCPPDQRDALNQAADDLNQRLQDLKER
                     TRVTNTEQLVFIAALNISYELAQEKAKTRDYAASMEQRIRMLQQTIEQALLEQGRITE
                     KTNQNFE"
     gene            48817..49000
                     /gene="ssrS"
                     /locus_tag="J1Y83_000043"
     ncRNA           48817..49000
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="J1Y83_000043"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            49021..49623
                     /locus_tag="J1Y83_000044"
     CDS             49021..49623
                     /locus_tag="J1Y83_000044"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HBA7628240.1"
                     /translation="MTQLPELPLTLSRQEIRKMIRQRRRALTPEQQQEMGQQAATRMM
                     TYPPVVMAHTVAVFLSFDGELDTQPLIEQLWRAGKRVYLPVLHPFSAGNLLFLNYHPQ
                     SELVMNRLKIHEPKLDVRDVLHLSRLDVLITPLVAFDEYGQRLGMGGGFYDRTLQNWQ
                     HYKMQPVGYAHDCQLVEKLPVEEWDIPLPAVVTPSKVWEW"
     gene            complement(49725..49784)
                     /gene="ibsC"
                     /locus_tag="J1Y83_000045"
     CDS             complement(49725..49784)
                     /gene="ibsC"
                     /locus_tag="J1Y83_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin IbsC"
                     /protein_id="HBA7628241.1"
                     /translation="MMRLVIILIVLLLISFPAY"
     gene            complement(50014..51246)
                     /locus_tag="J1Y83_000046"
     CDS             complement(50014..51246)
                     /locus_tag="J1Y83_000046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-3-phosphoglycerate dehydrogenase"
                     /protein_id="HBA7628242.1"
                     /translation="MAKVSLEKDKIKFLLVEGVHQKALESLRAAGYTNIEFHKGALDD
                     EQLKESIRDAHFIGLRSRTHLTEDVINAAEKLVAIGCFCIGTNQVDLDAAAKRGIPVF
                     NAPFSNTRSVAELVIGELLLLLRGVPEANAKAHRGVWNKLAAGSFEARGKKLGIIGYG
                     HIGTQLGILAESLGMYVYFYDIENKLPLGNATQVQHLSDLLNMSDVVSLHVPENPSTK
                     NMMGAKEISLMKPGSLLINASRGTVVDIPALCDALASKHLAGAAIDVFPTEPATNSDP
                     FTSPLCEFDNVLLTPHIGGSTQEAQENIGLEVAGKLIKYSDNGSTLSAVNFPEVSLPL
                     HGGRRLMHIHENRPGVLTALNKIFAEQGVNIAAQYLQTSAQMGYVVIDIEADEDVAEK
                     ALQAMKAIPGTIRARLLY"
     gene            complement(51502..52161)
                     /gene="rpiA"
                     /locus_tag="J1Y83_000047"
     CDS             complement(51502..52161)
                     /gene="rpiA"
                     /locus_tag="J1Y83_000047"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HBA7628243.1"
                     /translation="MTQDELKKAVGWAALQYVQPGTIVGVGTGSTAAHFIDALGTMKG
                     QIEGAVSSSDASTEKLKSLGIHVFDLNEVDSLGIYVDGADEINGHMQMIKGGGAALTR
                     EKIIASVAEKFICIADASKQVDILGKFPLPVEVIPMARSAVARQLVKLGGRPEYRQGV
                     VTDNGNVILDVHGMEILDPIAMENAINAIPGVVTVGLFANRGADVALIGTPDGVKTIV
                     K"
     gene            complement(52217..52447)
                     /locus_tag="J1Y83_000048"
     CDS             complement(52217..52447)
                     /locus_tag="J1Y83_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001328908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA7628244.1"
                     /translation="MHFAQRVRALVVLNGVALLPQFACKQGLANGELVRLFAPWSGIP
                     RPLYALFAGRKGMPAIARYFMDELTTRLANGV"
     gene            52588..53481
                     /locus_tag="J1Y83_000049"
     CDS             52588..53481
                     /locus_tag="J1Y83_000049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator ArgP"
                     /protein_id="HBA7628245.1"
                     /translation="MKRPDYRTLQALDAVIRERGFERAAQKLCITQSAVSQRIKQLEN
                     MFGQPLLVRTVPPRPTEQGQKLLALLRQVELLEEEWLGDEQTGSTPLLLSLAVNADSL
                     ATWLLPALAPVLADSPIRLNLQVEDETRTQERLRRGEVVGAVSIQHQALPSCLVDKLG
                     ALDYLFVSSKPFAEKYFPNGVTRSALLKAPVVAFDHLDDMHQAFLQQNFDLPPGSVPC
                     HIVNSSEAFVQLARQGTTCCMIPHLQIEKELASGELIDLTPGLFQRRMLYWHRFAPES
                     RMMRKVTDALLDYGHKVLRQD"
     gene            53684..54739
                     /locus_tag="J1Y83_000050"
     CDS             53684..54739
                     /locus_tag="J1Y83_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000073274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase"
                     /protein_id="HBA7628246.1"
                     /translation="MSNVQEWQQLANKELSRREKTVDSLVQQTAEGIAIKPLYTEADL
                     DNLEVTGTLPGLPPYVRGPRATMYTAQPWTIRQYAGFSTAKESNAFYRRNLAAHRGYD
                     SDNPRVAGDVGKAGVAIDTGEDMKVLFDQIPLDKMSVSMTMNGAVLPVLAFYIVAAEE
                     QGVTSDKLTGTIQNDILKEYLCRNTYIYPPKPSMRIIADIIAWCSGNMPRFNTISISS
                     YHMGEAGANCVQQVAFTLADRIEYIKAAISAGLKIDDFVHRLSFFFGIGIDLFMNAAM
                     LRAARYLWSEAVSGFGAQDPYNNVIRTTIDHCAHPMCRDYLHRYLENASGGHIHHDLS
                     HVFDLHRNLIATGSMLG"
     gene            complement(54736..55632)
                     /locus_tag="J1Y83_000051"
                     /pseudo
     CDS             complement(54736..55632)
                     /locus_tag="J1Y83_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417396.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            complement(55790..56530)
                     /locus_tag="J1Y83_000052"
     CDS             complement(55790..56530)
                     /locus_tag="J1Y83_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001520127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative stress defense protein"
                     /protein_id="HBA7628247.1"
                     /translation="MKFKVIALAALMGISGMAAQANELPDGPHIVTSGTASVDAVPDI
                     ATLAIEVNVAAKDAATAKKQADERVAQYISFLELNQIAKKDISSANLRTQPDYDYQDG
                     KSILKGYRAVRTVEVTLRQLDKLNSLLDGALKAGLNEIRSVSLGVAQPDAYKDKARKA
                     AIDNAIHQAQELANGFNRKLGPVYSVRYHVSNYQPSPMVRMMKADAAPVSAQETYEQA
                     AIQFDDQVDVVFQLEPVDQPPAKTPAAH"
     gene            complement(56623..57258)
                     /gene="argO"
                     /locus_tag="J1Y83_000053"
     CDS             complement(56623..57258)
                     /gene="argO"
                     /locus_tag="J1Y83_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine exporter ArgO"
                     /protein_id="HBA7628248.1"
                     /translation="MFSYYFQGLALGAAMILPLGPQNAFVMNQGIRRQYHIMIALLCA
                     ISDLVLICAGIFGGSALLMQSPWLLALVTWGGVAFLLWYGFGAFKTAMSSNIELASAE
                     VLKQGRWKIIATMLAVTWLNPHVYLDTFVVLGSLGGQLDVEPKRWFALGTISASFLWF
                     FGLAILAAWLAPRLRTAKSQRIINLVVGCVMWFIALQLARDGIAHAQALFS"
     gene            complement(57397..58257)
                     /locus_tag="J1Y83_000054"
     CDS             complement(57397..58257)
                     /locus_tag="J1Y83_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small-conductance mechanosensitive channel MscS"
                     /protein_id="HBA7628249.1"
                     /translation="MEDLNVVDSINGAGSWLVANQALLLSYAVNIVAALAIIIVGLII
                     ARMISNAVNRLMISRKIDATVADFLSALVRYGIIAFTLIAALGRVGVQTASVIAVLGA
                     AGLAVGLALQGSLSNLAAGVLLVMFRPFRAGEYVDLGGVAGTVLSVQIFSTTMRTADG
                     KIIVIPNGKIIAGNIINFSREPARRNEFIIGVAYDSDIDQVKQILTDIIQSEDRILKD
                     REMTVRLNELGASSINFVVRVWSNSGDLQNVYWDVLERIKREFDAAGISFPYPQMDVN
                     FKRVKEDKAA"
     gene            complement(58448..59527)
                     /gene="fbaA"
                     /locus_tag="J1Y83_000055"
     CDS             complement(58448..59527)
                     /gene="fbaA"
                     /locus_tag="J1Y83_000055"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016505616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HBA7628250.1"
                     /translation="MSKIFDFVKPGVITGDDVQKVFQVAKENNFALPAVNCVGTDSIN
                     AVLETAAKVKAPVIVQFSNGGASFIAGKGVKSDVPQGAAILGAISGAHHVHQMAEHYG
                     VPVILHTDHCAKKLLPWIDGLLDAGEKHFAATGKPLFSSHMIDLSEESLQENIEICSK
                     YLERMSKIGMTLEIELGCTGGEEDGVDNSHMDASALYTQPEDVDYAYTELSKISPRFT
                     IAASFGNVHGVYKPGNVVLTPTILRDSQEYVSKKHNLPHNSLNFVFHGGSGSTAQEIK
                     DSVSYGVVKMNIDTDTQWATWEGVLNYYKANEAYLQGQLGNPKGEDQPNKKYYDPRVW
                     LRAGQTSMIARLEKAFQELNAIDVL"
     gene            complement(59741..60904)
                     /gene="pgk"
                     /locus_tag="J1Y83_000056"
     CDS             complement(59741..60904)
                     /gene="pgk"
                     /locus_tag="J1Y83_000056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HBA7628251.1"
                     /translation="MSVIKMTDLDLAGKRVFIRADLNVPVKDGKVTSDARIRASLPTI
                     ELALKQGAKVMVTSHLGRPTEGEYNEEFSLLPVVNYLKDKLSNPVRLVKDYLDGVDVA
                     EGELVVLENVRFNKGEKKDDETLSKKYAALCDVFVMDAFGTAHRAQASTHGIGKFADV
                     ACAGPLLAAELDALGKALKEPARPMVAIVGGSKVSTKLTVLDSLSKIADQLIVGGGIA
                     NTFIAAQGHDVGKSLYEADLVDEAKRLLTTCNIPVPSDVRVATEFSETAPATLKSVND
                     VKADEQILDIGDASAQELAEILKNAKTILWNGPVGVFEFPNFRKGTEIVANAIADSEA
                     FSIAGGGDTLAAIDLFGIADKISYISTGGGAFLEFVEGKVLPAVAMLEERAKK"
     gene            complement(60954..61973)
                     /gene="epd"
                     /locus_tag="J1Y83_000057"
     CDS             complement(60954..61973)
                     /gene="epd"
                     /locus_tag="J1Y83_000057"
                     /EC_number="1.2.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="erythrose-4-phosphate dehydrogenase"
                     /protein_id="HBA7628252.1"
                     /translation="MTVRVAINGFGRIGRNVVRALYESGRRAEITVVAINELADAAGM
                     AHLLKYDTSHGRFAWEVRQERDQLFVGDDAIRVLHERSLQSLPWRELGVDVVLDCTGV
                     YGSREHGEAHIAAGAKKVLFSHPGSNDLDATVVYGVNQDQLRAEHRIVSNASCTTNCI
                     IPVIKLLDDAYGIESGTVTTIHSAMHDQQVIDAYHPDLRRTRAASQSIIPVDTKLAAG
                     ITRFFPQFNDRFEAIAVRVPTINVTAIDLSVTVKKPVKANEVNLLLQKAAQGSFHGIV
                     DYTELPLVSVDFNHDPHSAIVDGTQTRVSGAHLIKTLVWCDNEWGFANRMLDTTLAMA
                     TVAFR"
     gene            62345..62773
                     /locus_tag="J1Y83_000058"
     CDS             62345..62773
                     /locus_tag="J1Y83_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000988714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HBA7628253.1"
                     /translation="MMTLPYPYSSSARFYALRLLPGQEVLSQLRAFAQQQQLHAAWIA
                     GCTGSLTDVALRYAGQENTALLSGKFEVIALNGTLEQSGEHLHLCVSDPHGTMLGGHM
                     MPGCTVRTTLELVIGCLEELAFSRQSCALSGYDELHISPR"
     gene            62800..63378
                     /locus_tag="J1Y83_000059"
     CDS             62800..63378
                     /locus_tag="J1Y83_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HBA7628254.1"
                     /translation="MARRHFSSQALVLIVISIAINMIGGQLASMVKLPIFLDSIGTLI
                     SAVLLGPVIGMLTGLLTNLLWGLLTDPIAAAFAPVAMVIGLVAGWLARAGWFRTLPKV
                     VVSGVIITLAVTVVAVPLRTALFGGVTGSGADLFVAWMHSMGQNLVESVAITVIGANL
                     VDKILTAVIVWLLLRQLPIRTTRHFPAMAAVR"
     gene            63379..64086
                     /locus_tag="J1Y83_000060"
     CDS             63379..64086
                     /locus_tag="J1Y83_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HBA7628255.1"
                     /translation="MHPFTSLTLWALAACTTLILPTQTILPVYSAATFFCLIALKATR
                     RRAKYVVWLMFSLGAGLWLVHGGWLTEWLSGTPRSPERWAHAITLWLRILAIVSTSQL
                     WMQYVPVQRFIRALFASRLPPGVAYLFAGPLLVVEQLKRQLAIIHEAQRARGVPLDEG
                     WYQRLRAMPALIIPLTHNALNDLAVRGAALDMRAFRINNRRTTLWAPADSTLQRVARY
                     AMILLMLAEFGAWIWLR"
     gene            64074..64751
                     /locus_tag="J1Y83_000061"
     CDS             64074..64751
                     /locus_tag="J1Y83_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HBA7628256.1"
                     /translation="MVTLEQFRYCPTHSTHPPFCYDFHYVKPGMVAIFGDNGSGKSTL
                     AQLMAGWYPDFLPGEITGTGTLLGTPIGRLPLNEQSATIQLVQQSPYLQLSGCTFSVE
                     EEVAFGPENLCLAEKEIMARIDAALALTECQPLRHRHPATLSGGETQRVVIACAIAMQ
                     PKLLILDEAFSRLTPQAREMLLQRLQHWALERGSLIILFERHHTPFLNHCQQAWQLQN
                     GALQPLC"
     gene            64745..65422
                     /locus_tag="J1Y83_000062"
     CDS             64745..65422
                     /locus_tag="J1Y83_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/molybdate ABC transporter ATP-binding
                     protein"
                     /protein_id="HBA7628257.1"
                     /translation="MLTLNQISYRWPGAATDCLCDISLQLKQGEWLALTGDNGAGKST
                     LLRVMAGLLTPTAGTVMLQQQAMKNLKNRQRAAKIGVLFQEAENQLFHSTVADEIAFG
                     LKLQKCPADEITQRTNAALQCCQLADTASAHPLDLHSAQRRMVAVACLEALSPPLLLL
                     DEPSRDFDENWLSVFESWLEKCRQRGTSVVAISHDAAFTRRHFSRVVRLEDGVIRNVN
                     PSDDIHP"
     gene            complement(65394..66107)
                     /locus_tag="J1Y83_000063"
     CDS             complement(65394..66107)
                     /locus_tag="J1Y83_000063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside/nucleotide kinase family protein"
                     /protein_id="HBA7628258.1"
                     /translation="MKIELTVNGLKVQAQYSDDEIENVHKPLLRMLAALQTVNPQRRT
                     VVFLCAPPGTGKSTLTTFWEYLAQQDPELPAIQTLPMDGFHHYNSWLDVHQLRPFKGA
                     PETFNVAKLAENLRQVVEGDCTWPQYDRQKHDPVENALHVTAPLVIVEGNWLLLDDEK
                     WCQLAQFCDFSIFIKAPASALRERLVGRKLAGGLSLADAEAFYDRTDGPNVRRVLEES
                     LPANLTLMMTATGEYRLMD"
     gene            complement(66104..66613)
                     /gene="fumE"
                     /locus_tag="J1Y83_000064"
     CDS             complement(66104..66613)
                     /gene="fumE"
                     /locus_tag="J1Y83_000064"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarase E"
                     /protein_id="HBA7628259.1"
                     /translation="MATLTEDDVLEQLDAQDNLFSFMKTAHSILLQGIRQFLPSLFVD
                     NDEEIVEYAVKPLLAQSGPLDNIDVALRLIYALGKMDKWLYADITHFSQFWHYLNEQD
                     ETPGFADDMAWDFISNVNSIIRNASLYDALKAMKFADFAVWSEARFSGMVKTALTLAV
                     TTTLKELTP"
     gene            complement(66635..66950)
                     /locus_tag="J1Y83_000065"
                     /pseudo
     CDS             complement(66635..66950)
                     /locus_tag="J1Y83_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417405.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphatase class II"
     gene            complement(66947..68224)
                     /locus_tag="J1Y83_000066"
     CDS             complement(66947..68224)
                     /locus_tag="J1Y83_000066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HBA7628260.1"
                     /translation="MKTKVAAIYGKRDVRLREFELPEITDNELLVNVISDSVCLSTWK
                     AALLGSEHKRVPDDLENHPVITGHECAGVIVEVGKNLTGKYKKGQRFVLQPAMGLPGG
                     YSAGYSYEYFGGNATYMIIPEIAINLGCVLPYHGSYFAAASLAEPMCCIIGAYHANYH
                     TTQYVYEHRMGVKPGGNIALLACAGPMGIGAIDYAINGGIQPSRVVVVDIDDKRLAQV
                     QKLLPVELAASKGIELVYMNTKGVSDPVQTLRALTGDAGFDDVFVYAAVPSVVEMADE
                     LLAEDGCLNFFAGPTDKNFKVPFNFYNVHYNSTHIVGTSGGSTDDMKEAIALSATGQL
                     QPSFMVTHIGGLDAVPETVLNLPDIPGGKKLIYNGVTMPLTAIADFAEKGKTDPLFKE
                     LARLVEETHGIWNEQAEKYLLAQFGVDIGEAAQ"
     gene            complement(68239..69627)
                     /locus_tag="J1Y83_000067"
     CDS             complement(68239..69627)
                     /locus_tag="J1Y83_000067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICB"
                     /protein_id="HBA7628261.1"
                     /translation="MENKSARAKVQAFGGFLTAMVIPNIGAFIAWGFITALFIPTGWL
                     PNEHFAKIVGPMITYLLPVMIGSTGGHLVGGKRGAVMGGIGTIGVIVGAEIPMFLGSM
                     IMGPLGGLVIKYIDKSLEKRIPAGFEMVINNFSLGIAGMLLCLLGFEVIGPAVLIANT
                     FVKECIEALVHAGYLPLLSVINEPAKVLFLNNAIDQGVYYPLGMQQASVNGKSIFFMV
                     ASNPGPGLGLLLAFTLFGKGMSKRSAPGAMIIHFLGGIHELYFPYVLMKPLTIIAMIA
                     GGMSGTWMFNLLDGGLVAGPSPGSIFAYLALTPKGSFLATIAGVTVGTLVSFAITSLI
                     LKMEKTVETKSEDEFAQSANAVKAMKQEGAFSLSRVKRIAFVCDAGMGSSAMGATTFR
                     KRLEKAGLAIEVKHYAIENVPADADIVVTHASLEGRVKRVTDKPLILINNYIGDPKLD
                     TLFNQLTAEHKH"
     gene            complement(69655..70098)
                     /gene="cmtB"
                     /locus_tag="J1Y83_000068"
     CDS             complement(69655..70098)
                     /gene="cmtB"
                     /locus_tag="J1Y83_000068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IIA"
                     /protein_id="HBA7628262.1"
                     /translation="MRLIDYFPESSISVIHSAKDWQEAIDFSMVSLLDKNYISENYIQ
                     AIKGSTISNGPYYILAPGVAMPHARPECGALKTGMSLTLLKQGVYFPGNDEPIKLLIG
                     LSAADADSHIGAIQALSELLCEEEILEQLLTASSEKQLADIISRG"
BASE COUNT        17143 a        18586 c        17417 g        17003 t
ORIGIN      
        1 agtttatcaa actgaagaga gatagcctgc ctctttatct tatttctggt acttagcagc
       61 aaataaataa cgtgataaaa aaagccaaac gttttcgtat tttacaaaca accggaaact
      121 ggcatcaata tgtgatcaac cccacacatt atccgtcaaa ttagcctttc gcagccgcgt
      181 ggataattct ggcacactta ttgttagtcc caggtatagc tgtgaaaaca ccaatcactt
      241 tggcacgtca cagtgaaata aaccactttg cctgtcattc cactatcggg actttatgat
      301 gaaaactgtt aatgagctga ttaaggatat caattcgctg acctctcacc tgcatgaaaa
      361 agattttttg ttaacgtggg aacagacgcc agacgaactg aaacaagtac tggacgttgc
      421 cgccgcgtta aaagcactgc gtgctgaaaa catctcaacc aaagtcttta atagtggatt
      481 aggtatttcc gtattccgcg acaattccac ccgtacccgc ttctcttatg cttccgcgct
      541 taacctgctc ggccttgcac aacaagatct tgacgaaggt aaatcacaaa tcgctcacgg
      601 cgaaaccgtg cgtgaaaccg ccaatatgat ctccttctgc gctgacgcta tcggtattcg
      661 cgacgatatg tatctgggcg caggcaacgc ctatatgcgt gaagttggcg cggcacttga
      721 tgacggctac aaacaagggg ttctgccaca gcgtccggct ttagtgaacc tgcaatgcga
      781 tattgaccac ccgactcaat caatggctga cctggcatgg ttgcgtgaac actttggttc
      841 gctggaaaac ctgaaaggca aaaaaatcgc catgacctgg gcctactccc caagctatgg
      901 caaaccgctc tctgtaccac aaggcatcat cggtctgatg acccgcttcg gtatggatgt
      961 caccctggcc catccggaag gctacgacct gatcccggat gtcgttgaag ttgctaaaaa
     1021 caatgctaaa gcctccggtg gtagcttccg tcaggtcacc agcatggaag aagccttcaa
     1081 agacgcagac atcgtttatc cgaagtcatg ggcaccttac aaagtgatgg aagagcgtac
     1141 tgaattgctg cgtgcgaacg atcacgaagg cttaaaagca ctggaaaaac agtgtctggc
     1201 acagaacgcg caacacaaag actggcattg tactgaagag atgatggaac tgacccgtga
     1261 tggcgaagcc ctgtacatgc actgcctacc agctgatatc agcggcgtat cctgcaaaga
     1321 aggtgaagtg actgaaggcg tattcgaaaa ataccgtatc gctacctaca aagaagccag
     1381 ctggaagcct tatatcatcg ccgcgatgat cctgtcccgt aaatacgcca aaccaggtgc
     1441 actgctcgag caactgctga aagaagcgca agaacgcgtg aaataatccc tcgggccgat
     1501 cagctggtcg gtcctctatt tccagaggcc aaaaggatag gatatgtccg ttttctcatt
     1561 gaagattgat atcgccgata acaaattttt caacggcgaa acatcaccgc ttttttcgca
     1621 aagccaggca aaactggcac gccagttcca ccagaaaatt gctggttatc acccaacacc
     1681 gctttgtgcg ctggacgatc tcgcaaacct gtttggtgta aagaaaattc tcgtcaaaga
     1741 cgaatcaaaa cgattcggtc tgaacgcctt caaaatgctc ggcggtgcgt acgccatcgc
     1801 tcaattattg tgtgaaaaat atcatcttga tatcgaaacg ctgtcatttg agcacctgaa
     1861 aaatgccatc ggcgaaaaaa tgactttcgc gactaccacc gacggcaacc acgggcgcgg
     1921 tgtggcatgg gcagcacagc aactcggaca gaatgcggtg atttacatgc cgaaaggttc
     1981 tgctcaggaa cgcgttgacg ccattctaaa cctcggtgcc gagtgcatcg tcacggatat
     2041 gaactatgac gataccgttc gcctgaccat gcaacacgcg cagcagcacg gctgggaagt
     2101 ggtacaggac acggcatggg aaggctacac caaaatccca acctggatca tgcaaggcta
     2161 cgcaacactg gcggatgaag ccgtcgagca aatgcgtgaa atgggcgtaa ccccgacaca
     2221 cgttctgctg caagccggtg tcggcgctat ggccggcggc gtactaggtt atttggtgga
     2281 tgtttacacc ccgcaaaatc tgcacagcat cattgttgaa cctgacaaag ctgactgtat
     2341 ttatcgctcc ggcgtcaaag gtgacatcgt caacgttggc ggcgatatgg ccactattat
     2401 ggcgggcctg gcttgcggcg aacctaaccc gctgggctgg gaaatcctac gtaactgcgc
     2461 cacccaattc atctcctgcc aggacagcgt tgccgcatta ggtatgcgcg tgctgggtaa
     2521 tccgtacggt aacgacccgc gcatcatctc cggtgaatcc ggcgctgtcg gtttgggcgt
     2581 tctcgcagcg gttcattatc acccgcaacg tcaaagcctg atggaaaaac tggcgctgaa
     2641 caaagatgcc gtggtgctgg ttatcagcac tgaaggcgac accgacgtga agcactaccg
     2701 cgaagttgtc tgggaaggca aacacgctgt agcaccttaa tcaccctatt ggaactgctc
     2761 cctgcaaaac ggggagtaaa aaatctggag aaaaataatg gctaagaata ttccattcaa
     2821 actgattctt gaaaaagcaa aagattacca ggcagacatg actcgcttcc tgcgcgatat
     2881 ggttgctatt cccagtgaaa gctgcgacga gaaacgcgta gtacatcgta ttaaagaaga
     2941 gatggaaaaa gtcggcttcg ataaagttga aatcgaccca atgggtaacg ttctcggtta
     3001 tatcggtcac ggcccacgtc tggtagctat ggacgctcat atcgacaccg tcggcattgg
     3061 caacatcaaa aactgggact tcgatccgta tgaaggcatg gaaaccgacg agctgatcgg
     3121 tggtcgtggt acttccgacc aggaaggcgg catggcgtct atggtatatg ccggaaaaat
     3181 cattaaagat ctcggtctgg aagatgaata taccctgctg gttaccggta ctgtgcagga
     3241 agaagactgc gacggcctgt gctggcagta cattattgaa caatccggca ttcgcccgga
     3301 atttgtggtc agtaccgaac caaccgactg ccaggtatac cgtggtcaac gcggtcgtat
     3361 ggaaattcgt attgatgttc agggtgttag ctgccacggt tcggcaccag aacgcggtga
     3421 taacgccatt ttcaaaatgg gtccaattct tggcgaatta caggaactgt ctcaacgtct
     3481 gggctatgac gaattcctcg gcaaaggtac gctgactgtt tctgaaatct tcttcacctc
     3541 cccaagtcgt tgcgctgtag cagacagctg cgcagtttca atcgaccgtc gtctgacctg
     3601 gggcgaaacc tgggaaggcg cgctggacga gatccgcgct ctacctgcag tacagaaagc
     3661 taacgcggtt gtttctatgt acaactacga ccgcccgtcc tggactggcc tggtttaccc
     3721 aaccgaatgc tacttcccga cctggaaagt ggaagaagat cacttcaccg ttaaagcact
     3781 ggtgaatgct tatgaagggc tgtttggtaa agcgcctgtc gttgataagt ggaccttctc
     3841 gactaatggc gtatccatca tgggtcgcca cggcattccg gtcatcggtt ttggccctgg
     3901 taaagaacct gaagcgcacg cgccaaacga aaaaacctgg aaatctcacc tggtaacttg
     3961 tgccgctatg tacgctgcaa tcccattaag ctggctggca accgaataat tactcttcac
     4021 ttattccccc tccggtttac cggagggttt ttggagtttg ctatgcgcgt attgataaaa
     4081 aacggcattg ttgttaatgc agatggacag gcgaagcagg atttgctgat tgaaagcggg
     4141 attgttcgcc agttgggtac cgatatttcg ccgcaactcc cttgcgaaga aattgatgcc
     4201 tctggctgtt acgttttccc tggcggcgtg gatgtccata cgcatttcaa tattgatgtc
     4261 ggcatcgcgc gcagttgtga tgattttttt accggtaccc gcgctgctgc gtgtggcggt
     4321 acaacaacca ttattgacca tatgggattt ggcccaaatg gctgtcggtt acgccatcaa
     4381 ctggaagttt atcgcggtta tgccgcccac aaagcagtca tcgactacag ctttcacggt
     4441 gtcattcaac atattaatca cgctatcctc gacgaaattc cgatgatggt tgaggaagga
     4501 ctgagcagtt ttaaactcta tttaacctat caatataaac tcaacgatga tgaagtgttg
     4561 caggcattac gccgcttgca tgagtccggc gcgctgacca ccgtacaccc ggaaaatgac
     4621 gcagctatcg ccagcaagcg ggcggagttt atcgccgcag ggttaaccgc gccgcgttat
     4681 catgccttga gtcgacctct ggaatgcgaa gcggaagcca tcgcccgcat gattaacctg
     4741 gcacaaattg ccggtaacgc cccgctctat atcgtgcacc tgtctaacgg cttaggtctg
     4801 gattatctgc gtcttgcccg tgcgaatcac cagccagtct gggttgaaac ctgcccacaa
     4861 tatctcctgt tggacgaacg cagttacgat acagaagatg gcatgaagtt cattcttagc
     4921 ccaccgctgc gtaacgtacg cgagcaggac aaactgtggt gtggcatcag cgatggtgcg
     4981 attgacgtgg tggcaaccga tcactgcacc ttctcgatgg ctcaacgcct gcaaatttct
     5041 aaaggcgatt tcagccgctg cccaaatggc ttacccggtg tggagaaccg catgcaactg
     5101 ctgttctcca gtggcgtgat gacgggacgt atctcaccgg agcgttttgt tgagttaacc
     5161 agtgcaatgc ccgccaggct gtttggcctg tggccgcaaa aagggatatt agcgcccggt
     5221 tccgatggtg acgtggtgat tatcgaccca cgtcagagcc aacaaattca gcatcgccat
     5281 ctccacgaca acgctgacta ctcgccatgg gaggggttta cctgtcaggg cgcgattgtc
     5341 agaaccttat cccgtggtga aacgattttc tgcgacagca cctttacagg caaagccggg
     5401 cgaggtcgtt tcctgcgacg caaaccgttt gtccctcccg tgctctaacc actgccaggg
     5461 atctatacac aatcaagaat gatgagtgag gaaaaatgag taagaaaatt gttctcgccc
     5521 tgggcgggaa tgcgctgggc gatgacctgg ccggacaaat gaaagcggta aaaattactt
     5581 ctcaggcaat tgttgattta attgcccagg gacatgaggt catcgtcact catggcaacg
     5641 ggccacaggt gggcatgatt aaccaggcct ttgaagctgc cgcaaaaact gaagcgcact
     5701 cgccaatgct gccgatgtct gtctgtgttg ccttaagcca gggttatatt ggttacgatc
     5761 tacaaaacgc cttaagagaa gaactgcttt ctcgtggcat taataaacct gtagcaacgc
     5821 tggttaccca ggtcgaagtc gatgctaacg atccggcatt cctcaacccg accaagccga
     5881 tcggttcgtt ctttaccgag caagaagcag agctactgac aaaacagggt tacactctga
     5941 aagaagatgc gggtcgcggc tatcgtcgtg tcgttgcttc gccaaaaccg gttgatatca
     6001 ttgaaaaaga aacggttaaa gctctggtag atgccggaca ggtggtgatc accgttggcg
     6061 gtggtggtat ccctgttatt cgtgaaggta accatctgcg tggtgccagc gcggttatcg
     6121 ataaagactg ggccagcgcc cgtttagcag aaatgatcga tgccgatatg ctgatcattc
     6181 tgactgcggt agaaaaagtg gccattaact tcggaaaaga gaatgaacag tggctcgatc
     6241 gcctgtcgtt aagtgatgct gaacgcttta tcgaagaagg tcattttgcg aaaggttcta
     6301 tgctgcctaa agtggaagct gccgcctcgt ttgctcgctc tcgcgcaggt cgtgaagcac
     6361 tgattaccgt attgagcaaa gcgaaagaag ggattgaagg caaaaccgga acggtgattt
     6421 gtcagtaatt taaaggccgg atgggtaata ccatccggcc attttcattt aagccacttc
     6481 cagcacttct tttgtcgctt taacgccctg atgcatcaac atcattaacg cctcaagtac
     6541 gccgccgcca atcgcccggg ctttatcaga aacgctggtg aaatcagccg tttcaccacg
     6601 aggatcgata tcaccaattt taaaaccacc gaccacggcc agtccgtcgt tcaacaagcc
     6661 acgcaccatc cccgtcaacg gtgctttaat ttcatgctca ccaatccagg caatgacatc
     6721 gccctctttc accagatcgc ctaatttcac gttggatcgc ataatgcctg cagcaggagc
     6781 acggatcacc cgtcgggtgg tatgccccat aatattgccg ggaacaccgg tattctcctg
     6841 cgcacaacca gagtaaatca cctgaccgag ccagtgcccg cgatttgttt caattaccgc
     6901 atgacaatcc ttccctgcag taaagcccgg cccaagcgcg attgttactg gtgccatatc
     6961 tgcccgcgtt cccaaattct gtttcgccag aatagcgtcc accacgcaaa gcggtttcag
     7021 ttcatcaagc aatgaacagg tgggatctac catcacaggg ataaatccgc gttcggtaag
     7081 tttcatcgct tccgcagagc tggttgccag gcgggcggtg acgccttcga ccgtcatttc
     7141 gccatcgaac acggcctggg caaacgccac ggtacaacga atcactgtcg gtttttccac
     7201 ttccaacatg atcactttaa aacccgcatg atacagacgt agcgctacac cactggcgat
     7261 atctcccgca ccgcgaatca ccaccagctg gtgtccggaa ggatgagaag gcttcatcat
     7321 cagcccaccc ggcgcgttat ttttcacctg taatatttct gccagcacgc tgatggcgat
     7381 ctcctgcggc gtttccgcac ctatgttgta accaacgggc gcatgtaaac gggcaatatg
     7441 ttcttcagcc acgcctttct cacgcaattg gcgcaggaaa agctgaacct tgcggcgact
     7501 tgccagcaac cccagccatg caatgggttg ttcaatgagt ttgtcgaggg cttcacgatc
     7561 ctgattattc gtggcaatca ggacaaaatt atcagggcga atatccagtg cttccactgc
     7621 cgcaccaaac gactcagcat ggagaagcgt ggttgatggt gggaatagtt caggattgag
     7681 gctttcgcga taaatatcag caacggcgat atcaaatcct aatagcgccg cactctgggc
     7741 tatcgcccgg ttgacatgcc ccgcaccaat caacaccaga cgtggacgca taccatggac
     7801 gctgataaat actgacattg cacctccaca atctgacccg acagcatccg caccgttacg
     7861 agccatacgt ccatggaata atcgcggctt acgttcctgc aatgcctgaa gcgactcttc
     7921 aatcaccttc cgttcaacca ttccgccacc aattgtaccg acgatagaac catcggcgcg
     7981 cactaacatt tgtgcagaat ggcggggggt tgagcctcgg ctatcaacaa tttgcgccat
     8041 cgcaaacgga caattttgct cttcgagttt tgcagcctct gtgaaaatat tcataccaac
     8101 ctcaatggat atcctttcag taacccggaa tacccgggcc gctaattcac aaatctgcgc
     8161 gcgattgcag gatgttcttg tatatcgccc agccagattg cctctacgtt gtgttgttgc
     8221 agcggttgaa ggagctcgct ttgcgcaatc gcattctcac attgagaaaa acggttaatg
     8281 aaccagactc gccgacaacc ttgcggtacg tttttaaacg ccccctgagg gtggcgaacc
     8341 agcgcaacga gatcgctcag ttgcaagggt gcatcaggtg ttaacccagt aatatcagca
     8401 aactgcgacc agcgatggac attttctgtg ctcactttcg cacccaaaat atgtcctccc
     8461 atcacagcaa tcacgcagca actgctttta ggtatgcaag gttcgtgctc atcaggcgct
     8521 tttaacggca ttccacgcga gccatctgcc tcaatgagaa ttacgtcaca ctctggtcgt
     8581 tgtaccagtg catcaatcgc ttctggcgta aatccctgca cttttccctg gttcgctttc
     8641 cagcagtgaa aacaaaatga aatgggggat ataaaagacg catgaggaag catggcggga
     8701 tcacgacaga aaaccacggg ccagtgagat gttggcataa acatatgtgt cgtcgtagtg
     8761 attaatacac gcctgccgct cgcctggaac agttctgcca gccaaaaaag caagctggtt
     8821 ttccccccag caccaacaat agaaatcaca gtgggacgtt tctgcgcgcc taaatcaatg
     8881 aataatgccg atgggtcaat tatacttttc acgcgtcttt atggacctct ctgggataaa
     8941 ttaccctcaa cgtaatccat caggaagtaa cgcaattatc aggcgttatt agctcctgta
     9001 aataatggaa tcactatgtc agccatcgac tgtataatta ccgccgcagg attatcatca
     9061 agaatggggc aatggaaaat gatgttaccc tggcaacagg gaacaattct tgatacaagt
     9121 atcaaaaatg cgttgcagtt ttgtagccga attattttag tcaccggcta tcgtggtaat
     9181 gaactgcacg aacgttatgc gaaccagagc aatatcacta ttatccacaa cccagattat
     9241 gcgcagggtt tactaacatc agtaaaggcc gcagtacccg cggtgcaaac agaacattgt
     9301 tttctcaccc acggagatat gccaaccctc accatcgata tttttagaaa aatctggtcg
     9361 ttacgaaatg atggcgcaat actgccactc cataatggca tccccggcca tccgatttta
     9421 gtgtcaaaac catgcctgat gcaggcaatc cagcgaccca atgtcaccaa tatgcgtcag
     9481 gcactcctta tgggagaaca ttattccgtc gaaatagaaa atgcagaaat aattttagat
     9541 attgatactc cggacgactt tattacagcg aagaaaaggt atactgaaat ttagaaaacg
     9601 tagttaaacg attgcgttca aatatttaat ccctcctgca ctctttatca taatgataag
     9661 gaatcactga ttttgagaaa agagaaaaag tgataaatgt gctggcaata ataattttca
     9721 ggagtgattt taattctcat ttagcacctg tgagttcatt cacaaaaagc cattaagttt
     9781 cgagccgcct cacatttttt tatatttccc gccaaacctg gcaagagtgg tgcgattgtt
     9841 gctctatccc cctaaaccac cggatttctc aacaccggtc actcaatgat atctgtataa
     9901 gctaaggaga gggttatggg ggatattatg cgtcccattc cgtttgagga acttttgacg
     9961 cgcatatttg atgaatacca acaacaacgc tcaatctttg gtattcccga gcaacagttt
    10021 tactcacccg taaaaggtaa aactgttagc gtcttcggtg aaacctgtgc cactcccgtc
    10081 ggccctgccg ctggcccgca cacgcaactt gcgcaaaaca tcgtcacctc ctggctgact
    10141 ggcggacgct tcatcgaact aaaaaccgtc caaattcttg accgcctgga gctggaaaag
    10201 ccctgtatcg atgccgaaga cgagtgcttt aacaccgaat ggtctaccga atttaccctg
    10261 cttaaagcct gggatgaata cctcaaagcc tggtttgccc tgcaccttct cgaagcgatg
    10321 ttccagcctt ctgattccgg taaatcgttc atctttaata tgagcgtcgg ttacaacctc
    10381 gaaggtatta agcaaccgcc gatgcagcag ttcatcgaca atatgatgga cgcatctgac
    10441 catccgaaat tcgctcaata ccgcgatgca cttaataagc tactccagga tgaggcattt
    10501 ttagcccgcc acggattgca ggaaaaacgc gaaagcttgc aagccttacc cgctcgcatc
    10561 cccaccagca tggtacaagg ggttaccctc tccaccatgc acggctgtcc tccgcatgaa
    10621 atcgaagcca tttgccgcta catgctggaa gaaaaagggc tcaacacctt tgtgaaactc
    10681 aacccgacct tactggggta cgcgcgtgtt cgtgagatcc tcgatgtctg cggtttcggt
    10741 tacatcggct taaaagaaga gtcatttgat cacgacctca agctgacgca agcactggaa
    10801 atgctggaac gcctgatggc actggcaaaa gaaaaatcac tcggctttgg cgtaaaactg
    10861 actaacactc tcggcaccat caacaataaa ggcgcattgc ccggtgaaga gatgtatatg
    10921 tcaggccgtg cgctgttccc gctctccatc aacgttgcag cagttctctc tcgcgccttt
    10981 gacggcaaac tgcccatttc ttattccggt ggtgccagtc agctgactat ccgcgatatt
    11041 tttgatactg gtattcgccc tattactatg gcaaccgacc tgctgaaacc tggcggctat
    11101 ctgcgcttaa gtgcctgcat gcgcgagctg gaaggctcag atgcctggga gcttaaccac
    11161 gttgacgtcg aacgactgaa cagactggca gcagacgcgt taaccatgga atacacccag
    11221 aaacactgga agccagaaga gcgtattgaa gtggctgaag acctgccgct gaccgattgc
    11281 tacgttgccc cctgtgttac tgcctgcgct atcaagcaag atattccgga atacatccgt
    11341 ctgcttggcg aacaccgcta tgccgacgcg ctggaactca tctatcaacg caacgccctg
    11401 cccgccatta ccggtcatat ttgcgatcac cagtgccaat acaactgtac ccgcctggat
    11461 tacgacagtg cgctgaatat ccgcgaactg aaaaaagtcg ccctggaaaa aggttgggat
    11521 gaatataagc aacgctggca caaaccagcc ggttctggtt cacgccatcc ggtagccgtg
    11581 attggtgcag gtccggcggg tctggccgca ggttacttcc ttgccagagc gggccatccg
    11641 gttacgctgt ttgaacgcga agccaatgcg ggcggcgtgg tgaaaaatat cattcctcag
    11701 ttccgtattc ctgcagagtt aattcagcac gatatcgatt ttgttgccgc tcacggcgtg
    11761 aaatttgagt acggttgctc acccgatttg accgtcgaac agttaaaaaa tcagggcttc
    11821 cactatgttc tgattgccac cggtaccgat aaaaatagcg gtgtgaaact ggcgggcgac
    11881 aaccaaaatg tctggaaatc actccccttc ctgcgtgaat acaacaaggg caccgcgctc
    11941 aagctgggca aacatgtggt cgttgtcggg gcgggtaaca cggcaatgga ctgcgctcgt
    12001 gcggcgttac gcgttccagg cgtagaaaaa gcaacggtcg tttaccgtcg ttcactgcaa
    12061 gagatgccag catggcgcga agagtatgaa gaagcgttgc acgacggcgt ggagttccgt
    12121 tttctgaata atccggaacg tttcgatgct gatggcacct taaccttgcg cgttatgtcg
    12181 cttggcgaac ctgatgagaa aggtcgtcgc cgtccggttg aaaccaacga aacagtaacg
    12241 ctgcatgtag acagcctgat caccgccatt ggtgaacagc aggatactga agccctgaat
    12301 gcaatgggcg tgccgctgga caaaaacggc tggccagacg tcgaccataa tggcgaaaca
    12361 cgtctgactg acgtctttat gatcggcgac gtacagcgcg gaccatcctc cattgtcgct
    12421 gctgtcggaa ccgcgcgtcg ggcgaccgat gccatcctga gtcgggaaaa tatccgttcc
    12481 caccagaacg ataaatactg gaataacgtc aatccggcgg aaatctatca acgtaaaggc
    12541 gatatctctg tcactctggt gaacagtgac gatcgtgacg cgtttgtcgc gcaggaagcc
    12601 gctcgctgcc ttgaatgtaa ctacgtttgc agcaagtgtg tggatgtctg cccgaaccgc
    12661 gccaacgtat ccattgcggt cccaggcttc cagaaccgtt tccagacgct gcacctcgac
    12721 gcctactgca acgaatgcgg caactgcgcc caattctgcc cgtggaacgg taaaccgtac
    12781 aaagacaaaa tcaccgtctt cagcctgtcg caagactttg ataacagcag caacccaggc
    12841 ttccttgtgg aagattgccg ggtacgcgta cgtctgaata accaaagctg ggtgttaaac
    12901 atcgacagcg aaggtcagtt caacaacgta ccaccggagc tgaacgatat gtgccgcatt
    12961 attagccatg tccaccagca tcatcattat ctgctgggcc gcgtggaggt gtaatcatgt
    13021 tgattctgaa gaatgtcacc gcagtgcagt tacacccggc gaaagtgcag gaaggcgttg
    13081 atatcgccat cgaaaatgat gtgattgtcg ctatcggcga tgccctgacg caacgctacc
    13141 ccgatgccag ctacaaagag atgcatggtc ggattgtgat gccgggaatt gtctgctcgc
    13201 ataaccattt ttactcgggg ctttcccgcg gaattatggc aaacatcgcc ccctgtccgg
    13261 atttcatctc aacgctgaaa aatctctggt ggcggctcga tcgcgccctt gatgaagagt
    13321 cgctctatta cagcggactg atttgttccc tggaagcgat taagagcgga tgtacatcgg
    13381 ttatcgatca ccatgcctct ccagcctata tcggcgggtc gctctccaca ttgcgcgacg
    13441 catttttaaa agttggcctg cgcgcgatga cctgttttga aactactgac cgtaacaacg
    13501 gcatcaaaga gttgcaggaa ggtgtagaag aaaatatccg cttcgcccgt cagattgatg
    13561 aggcgaagaa agcggcaact gaaccgtatc tggtggaagc gcatatcggt gctcacgcgc
    13621 cgtttaccgt gccggatgcc ggtctggaga tgctgcgtga agccgtgaaa agcacaggtc
    13681 gtggtttgca tattcacgct gcggaagacc tttacgacgt ttcctacagt caccattggt
    13741 acggcaaaga cttgctggcg cgactggcgc aattcgatct catcgacagc aaaacgctgg
    13801 tcgcgcacgg gctgtatttg tcgaaagacg acatcacgct gctcaatcag cgtgatgcgt
    13861 tcctggtgca taacgcccgt tcaaacatga acaaccatgt cggctacaac catcacctta
    13921 gcgacatccg caatctggcg ttgggaacgg acggcattgg ctccgatatg tttgaagaga
    13981 tgaaatttgc cttctttaaa catcgcgatg cgggtggtcc gctgtggcct gacagttttg
    14041 ccaaagcact ggctaacggc aacgaactga tgagccgcaa ctttggcgcg aaatttggcc
    14101 ttctggaagc cggttataaa gccgatttaa ccatttgcga ttacaactcg ccgacgccgc
    14161 tgctggcaga caatatcgcc ggacatatcg ctttcggtat gggctcaggc agcgttcaca
    14221 gcgtgatggt caatggtgtg atggtctatg aagagcgtca gtttaacttc gattgcgatt
    14281 ccatttatgc gcaagccaga aaagccgctg ccagtatgtg gcgtcggatg gatgcgctgg
    14341 cataaataac agctcccctc ttctttcaga gaagagggga aattaaggaa agacgatgat
    14401 tgaacaattt ttcagacccg actctgtcga acaggcgctg gaactgaagc gccgctacca
    14461 ggatgaagcc gtctggttcg ccgggggcag caaactcaat gctacaccaa cccgtaccga
    14521 taaaaagatt gccatttcct tacaggatct ggaactggac tgggttgact gggataacgg
    14581 tgcactgcgg attggtgcaa tgtctcgctt gcagccactg cgtgatgcgc gatttattcc
    14641 tgcagcactg cgtgaagctc tcggttttgt ctactctcgc catatccgta atcagtcgac
    14701 cattggtggt gaaatcgccg cccggcagga agagtcggtg ctgcttcccg tcctgctggc
    14761 actggatgct gaactggttt ttggcaacgg cgaaacgctg tcaatcgagg actacctagc
    14821 ctgcccatgc gatcgcctgt taaccgaaat tatcattaaa gatccgtatc gcacctgtgc
    14881 gactcgcaaa attagccgtt ctcaggcagg tttaaccgtc gtgacggcag ccgttgcaat
    14941 aacagaccac gacggtatgc gaattgcgct ggatggcgtc gccagtaaag cactgcgtct
    15001 gcatgatgtt gaaactcaaa atctggaagg caatgcactt gaacaggctg tcgccaacgc
    15061 cattttcccg caggaagatt tgcggggcag cgtggcctat aaacgctata tcacgggagt
    15121 tctggtagcc gatctgtatg acgactgcca acaggctggg gaggaagccg tatgatcatc
    15181 cactttactt taaatggcgc gcctcaggag ctaaccgtta atccaggcga aaacgtgcaa
    15241 aagctgttgt ttaacatggg aatgcactct gtacgcaaca gtgatgatgg ttttgggttt
    15301 gccggttctg acgcaattat ctttaacggt aatatcgtta acgcgtcctt gcttatcgcc
    15361 gcacagttag agaaggcaga tattcgtacc gcagaatctc tgggcaaatg gaatgagtta
    15421 agtctggttc aacaggccat ggttgatgtt ggcgtggtgc agtctggtta taacgatcca
    15481 gctgcggctc tgattatcac cgatcttctc gatcgcatcg acgcacctac ccgcgaagag
    15541 atcgacgacg cactctcagg tctgtttagc cgcgatgctg gctggcagca atactatcag
    15601 gtcattgaac tggcggttgc acgtaaaaat aatccgcagg ccaccattga tatcgctccg
    15661 actttccgtg acgaccttga agtcattggc aagcattatc ctaaaactga tgccgcgaaa
    15721 atggtgcagg cgaaaccctg ctatgttgaa gatcgcgtaa cggctgacgc ctgcgtcatt
    15781 aaaatgttac gtagcccaca cgctcacgca ctgattactc atctggatgt cagcaaagct
    15841 gaagccttac cgggcgtcgt tcacgttatt actcacctga attgcccgga tatttactat
    15901 accccgggtg gtcagagcgc accggaaccg tcaccgcttg accgccgaat gttcggcaag
    15961 aaaatgcgtc acgtcggcga tcgcgttgct gcggtagtag ccgaaagtga agacattgcg
    16021 ctcgaagcac tgaagctcat cgacgttgaa tatgaagtgc ttaagccggt aatgtcgatt
    16081 gacgaggcaa tggcggaaga tgcgcctgtc gtgcacgatg aaccggtggt gtatgttgct
    16141 ggtgcgccag atactctgga agatgataac agccatgcag cccagcgcgg cgagcatatg
    16201 atcatcaact tcccgatcgg ttctcgccca cgcaaaaata tcgctgccag tattcatggt
    16261 catattggcg atatggacaa aggctttgcc gatgccgatg tgatcattga gcgaacctat
    16321 aactcaacgc aggcgcagca gtgcccgact gaaacacata tctgctttac ccgtatggac
    16381 ggcgatcgtc tggtgatcca cgcctccacc caggtaccat ggcacttacg ccgccaggtc
    16441 gcgcgcctcg tgggcatgaa acaacataaa gttcatgtca ttaaagaacg tgtaggcggc
    16501 ggttttggtt ccaaacagga catcctgctg gaagaagtgt gcgcctgggc aacctgcgtg
    16561 accgggcgtc cggtactgtt ccgctacacc cgtgaagaag agtttattgc taacacctct
    16621 cgtcacgtcg cgaaagtcac cgtcaaactg ggcgcgaaaa aagatggtcg cctgacggca
    16681 gtgaagatgg atttccgcgc caacacgggt ccttacggta accactcact caccgtaccg
    16741 tgtaacggac cggcgctgtc gctgccgtta tatccatgcg ataacgtcga ttttcaggtc
    16801 accacctact acagcaacat ttgcccaaat ggtgcttatc agggttatgg cgcaccgaaa
    16861 ggtaacttcg ctatcaccat ggcattagcg gaactggctg aacagttaca gatcgaccaa
    16921 ctggaaatta tcgaacgtaa ccgggtacac gaagggcaag agctgaaaat tctcggtgca
    16981 atcggtgaag gtaaagcgcc gacctccgtt ccttccgccg ccagctgcgc actggaagag
    17041 atcctgcgtc agggtcgcga gatgatccaa tggtcttcac caaaaccgca aaatggtgac
    17101 tggcacatcg gtcgcggcgt cgccattatc atgcagaaat cgggtatccc ggatatcgat
    17161 caggctaact gcatgatcaa actggaatcg gatggcacct ttatcgttca ttccggtggt
    17221 gcggatattg gtactggtct ggatactgtg gtgaccaaac tggcagcaga agtgctgcac
    17281 tgccccccgc aggacgtgca tgttatctct ggtgataccg atcatgcact gtttgataaa
    17341 ggcgcatatg cctcgtccgg tacttgcttc tcgggtaacg ctgcgcgttt ggcagcggaa
    17401 aatctgcggg agaaaatcct gttccacggc gcgcaaatat tgggtgagcc agtggcagat
    17461 gttcaactag caacgccggg cgtcgttcgc ggcaagaaag gcgaagttag tttcggagag
    17521 atagcccata aaggcgaaac cggcaccggc tttggttcac tggtgggaac tggcagttat
    17581 atcacacctg atttcgcctt cccgtatggc gcaaacttcg ctgaagttgc cgtcaacacg
    17641 cgtacgggtg aaatccgcct ggataaattc tacgccttgc tggactgcgg tacaccggtc
    17701 aatccagagt tagcgttggg acaaatctac ggtgccactc tgcgagctat cggccacagt
    17761 atgagcgaag agatcattta tgacgccgaa ggtcacccgt taacgcgtga tttacgcagt
    17821 tacggcgcac cgaaaattgg tgacattccg cgtgatttcc gcgctgtgct ggtgccgagc
    17881 gacgataaag tcggcccatt cggggcgaaa tcgatctcgg aaatcggtgt aaatggcgca
    17941 gctccggcga ttgctaccgc aattcacgat gcctgcggca tctggttacg cgaatggcat
    18001 ttcacaccgg agaaaatact caccgcgctg gaaaaaatat aagtaataaa tggccgcttc
    18061 ggcggcctgt ttctctgttt gaacgatgac taaatagtga ctgcgaaaat gagataagcc
    18121 gttctcaagg cgtcctgcgc cccaaattcg tgggaattta ttttactaat tcagatgatc
    18181 aaatttactt taaaaggagt gaagggatgt ctgatataaa ccatgcaggt tctgacctta
    18241 tatttgaact ggaggatcgc cctccctttc atcaggctct cgtaggtgcc attacccatc
    18301 tgttggcaat tttcgttccg atggtaaccc ccgcgttaat cgtaggtgcg gccttacagc
    18361 tttccgctga aacaactgcc tatcttgttt ctatggcgat gatcgcctct ggtattggta
    18421 cctggttaca agtaaaccgc tacggcatcg tcggttctgg tttactttca attcagtcag
    18481 tcaatttttc atttgttacg gtcatgattg cgctgggcag cagcatgaaa agcgacggtt
    18541 ttcacgaaga gttaatcatg tcgtcgctcc tcggcgtctc cttcgttggc gcatttctgg
    18601 ttgtcggctc ttcttttatc ctgccctatt tacgtcgggt tattacgcct accgtcagcg
    18661 gcattgtggt gctgatgatc ggcttaagcc tgattaaagt cggcattatt gattttggtg
    18721 gaggatttgc agccaaaagc agcggtacgt tcggcaatta cgaacatctc ggcgttggtt
    18781 tattggtttt gattgtggtg atcggcttta actgctgccg cagtccgttg ctacgcatgg
    18841 gagggatcgc cattgggcta tgtgtcggct atatcgcatc gttatgcctg ggcatggtgg
    18901 atttcagcag tatgcgcaat ttgccgttaa tcaccatccc gcatccgttc aaatacggct
    18961 ttagttttag cttccatcag ttcctggtgg ttggcacgat ttatctgctt agcgtgctgg
    19021 aagctgtcgg cgatatcacc gccacggcaa tggtttcccg ccgtcccatt cagggggaag
    19081 agtatcagtc ccgactgaaa ggcggcgtgc tggcagatgg tctggtttct gttatcgcct
    19141 ccgctgtcgg ttcattacca ttaaccacgt ttgcgcaaaa taatggggtt attcagatga
    19201 ctggcgtcgc ttcacgttat gtcgggcgaa ccatcgcggt aatgctggtt atcctcggct
    19261 tatttccgat gattggcggc ttcttcacga ccattccctc agcagttctg ggaggcgcaa
    19321 tgacgttgat gttttccatg attgccatcg cagggattcg catcatcatc accaacggtt
    19381 taaagcgccg agaaacactt attgtcgcca cgtctttagg tttaggactt ggcgtctcct
    19441 acgatcccga aatttttaaa atattgccag cctctattta tgtattagtt gaaaacccta
    19501 tttgtgctgg cgggttaact gcgattttat taaatattat cctccctggt ggctaccgac
    19561 aggaaaacgt tctgcctggt attacctcag cggaagagat ggattaacag taaaggagtc
    19621 aatgatgtca ggagaacaca cgttaaaagc ggtacgaggc agttttattg atgtcacccg
    19681 tacggtcgat aacccggaag aaattgcctc tgcgctgcgg tttattgagg atggtttatt
    19741 actcattaaa cagggaaaag tggaatggtt tggcgaatgg gaagacggaa agcatcaaat
    19801 tcctgacacc attcgcgtgc gcgactatcg cggcaaactg atagtaccgg gctttgtcga
    19861 tacacatatc cattatccgc aaagtgaaat ggtgggggcc tatggtgaac aattgctgga
    19921 gtggttgaat aaacacacct tccctactga acgtcgttat gaggatttag agtacgcccg
    19981 cgagatgtcg gcattcttca tcaagcagct tttacgtaac ggaaccacca cggcgctggt
    20041 gtttggcact gttcatccac aatccgttga tgcgctgttt gaagccgcca gtcatatcaa
    20101 tatgcgtatg attgccggta aagtgatgat ggaccgaaac gcaccggatt atctgctcga
    20161 cactgccgaa agcagctatc accaaagcaa agaactgatt gaacgctggc acaaaaatgg
    20221 tcgtctgttg tatgcgatta cgccacgctt cgcccctacc tcatctcctg aacagatggc
    20281 gatggcgcaa cgcctgaaag aagaatatcc ggatacatgg gtacataccc atctctgtga
    20341 aaacaaagat gaaattgcct gggtgaaatc gctttatcct gaccatgatg gttatcttga
    20401 tgtttaccat cagtacggcc tgaccggtaa aaactgcgtc tttgctcact gcgtccatct
    20461 cgaagaaaaa gagtgggatc gtctcagcga aaccaaatcc agcattgctt tctgtccgac
    20521 ctccaacctt tacctcggca gcggcttatt caacttgaaa aaagcatggc agaagaaagt
    20581 caaagtgggc atgggaacgg atatcggtgc cggaacaact tttaacatgc tgcaaacgct
    20641 gaacgaagcc tacaaggtat tgcaattaca aggctatcgc ctctcggcat atgaagcgtt
    20701 ttacctggcc acgctcggcg gagcgaaatc tctgggcctt gacgatttga ttggcaactt
    20761 tttacctggc aaagaggctg atttcgtggt gatggaaccc actgccactc cgctacagca
    20821 gctgcgctat gacaactctg tttctttagt cgacaaattg ttcgtgatga tgacgttggg
    20881 cgatgaccgt tcgatctacc gcacctacgt tgatggtcgt ctggtgtacg aacgcaacta
    20941 ataataaaac tttaacatcc tcgtgaggac atcattatgt ctggagacat cctacaaaca
    21001 ccggacgcac caaagccaca gggcgcgctg gataattatt ttaaaattac cgctcgtggc
    21061 agtaccgttc gtcaggaagt actggctggc ttaacgacct ttctggccat ggtttattcc
    21121 gttatcgtcg ttccgggaat gctgggcaaa gcaggttttc ctcccgcagc tgtgtttgtt
    21181 gccacctgtc tggtcgcggg cttcggctcg ttgctgatgg ggttatgggc caatttgcca
    21241 atggcgattg gttgcgctat ttccttgacg gcgtttaccg cattcagtct ggtactcggg
    21301 caacaaatta gcgttcctgt cgcactgggc gcagtctttc tgatgggcgt catcttcacc
    21361 gctatttccg taaccggtgt gcgtacctgg atcttacgta atttgccgat ggggatcgct
    21421 cacggtacag gtatcggtat cggcctgttt ctgctgctga ttgctgctaa cggtgtggga
    21481 atggttatca aaaacccgat tgaaggcttg ccagtggcgc tcggtgcctt tacctccttc
    21541 ccggtgatga tgagcttact ggggctggcg gtcatcttcg gtctggaaaa gtgccgcgta
    21601 cccggcggga tcttgttggt gattattgca atttcgatca tcggcttaat ctttgaccca
    21661 gcggtgaagt accacggtct ggtggcaatg ccaagcctga ctggcgaaga tggtaagtct
    21721 ctgattttca gcctcgatat tatgggcgca ctccagccaa ctgtacttcc gagtgtactg
    21781 gcattggtga tgaccgcagt gttcgacgcc actggcacca tccgtgccgt cgccggtcag
    21841 gcgaatttgt tggataaaga caaccagatc atcaacggcg gcaaagccct gaccagtgac
    21901 tcagtaagtt caatattctc cggcctggtg ggcgcagcgc ccgcggcggt ttatatcgaa
    21961 tcagcggcag gaaccgccgc cggggggaaa accggtttaa ccgcaaccgt ggttggtgtg
    22021 ttgttcctga tgattctgtt tttatcaccg ttatcgtttc tgatccccgg ttatgccaca
    22081 gctcccgcac tgatgtacgt aggtttgctg atgttaagca acgtctcgaa gctggatttc
    22141 aacgatttta ttgacgcaat ggctggcctg gtgtgtgccg tgttcatcgt tctgacttgt
    22201 aatatcgtta ccggtattat gctgggcttt gtaacactgg tggtgggtcg cgtctttgcg
    22261 cgcgaatggc aaaagctgaa tattggtacg gtgatcatta ctgccgcact ggtcgcgttt
    22321 tacgcgggtg gttgggctat ctaatagatt ctccgcgcct ttcaacgcgg agttttttat
    22381 caaagaggat tcgcgccgct ggcagcaata cgctgctggc ggatacgcct gagttctctt
    22441 tcagtcagca actgcaacgc ctgtgtcggg caagattcaa cgcaggctgg cccctcttcc
    22501 cgctgttcac agagatcgca tttcacgatt tgttgccggg tatcacccgg caatgactga
    22561 atggtgatca tcccaaacgg gcacgcgcta acgcaactct gacagccaat acaacgggca
    22621 gaattggcct gcaccacctg ctcgcccatc gtcagcgccc ccacggggca agcgccaaca
    22681 caaggggcgt tttcacactg atggcacatc accggcgcac tgatgctatc cagccgttgt
    22741 accttcagcc ggggtaaaaa gatatcggca ttcaactcct gttctgaagg gtgagcgacc
    22801 acacaggcca cttcacaagt acggcagcca atacagtcag ccggattaac gataattaac
    22861 gatttcatca cgatgccttc gtatcaaaca gagttaacat atcgcgcgcc gcctgccttc
    22921 ctgcggccat tgcagtgaca accagatccg cgccatgaac tgcatcacca ccagcaaaga
    22981 cttttttcag atgcgtctgg gtaggtaaat acccgacgtc gccggtttga atcaggcccc
    23041 atttatcgag tttaattccg ctgccctgca accacggcat ggtatgtgcc tggaaaccaa
    23101 aggccataat gagaacatcg gcgggcaatt caaactctga acccgcaacc ggacgaggac
    23161 gacggcgacc atccggcccc ggctcaccca tagcggtacg aatcaggccc accgccgtta
    23221 agcgtccatc ttcatcacaa gcgatatatt gcggctgaac attgaactga aactcaacac
    23281 cttcctcgcg ggcattgacc acctctttgc gcgagcccgg catactgact tcatcacgac
    23341 gatacgcaca ggtcacgctg gcggcattaa ggcggatgga agtccgcaaa caatccattg
    23401 tcgtatcgcc accgcccaat accacaactc gcttaccttc cacatccgtc agcggatact
    23461 ctgcagactc cggcaatccc atgagctggc gggtatgggc ggtcaggaac ggtaacgcct
    23521 gaatgacacc aggcgcatct tcatgcggca gatctgctcg catcatcccg taagtcccca
    23581 cgccgaggaa aacagcatca tattccgccg ttaattcgtt gaaagagata tcgcggccaa
    23641 tttcacagtt aagatgaaag tcgattccca ttgcggtgaa tatctctcgc cgctggctta
    23701 ataccgtttt atcgagtttg aaaggaggaa tgccaaaagt cagcataccg ccaatttctg
    23761 gatggcgatc aaagacatca acctgaactc ccgcgcgcgc cagaatatca gcacacccta
    23821 atcctgcagg tccagcgcca atcaccgcca ctttttcgat acggggaaca actttgctga
    23881 catcgggacg ccagcccatc gccagcgcag tatcggtgat gtagcgttcc agattaccga
    23941 tagagactgc gccagagtga tctttcaaag tacatgcgcc ttcacaaaga cggtcctgtg
    24001 gacataccct gccgcagatt tcgggtaagg aactggtctg gtggcaaagt tctgccgctt
    24061 caataatctt tccctcctgt accagacgga tgtaatccgg aatagcgtta tgcagcggac
    24121 aatgccagtt gcagttagct ttttcggcac aataaacaca gcggtcactc tcataagtcg
    24181 cttgttgtgg atccagcccg caatagattt cgccaaagtg ggttttccgt tcactcgctg
    24241 aaattttatc tgcgccctta cgcgagttaa cggggagcaa cgctgcactg cgagatggct
    24301 gagcgtctga tgacgctttt cctgctgccg ttttgcgctg gcgggccacc tttatctgct
    24361 gcaacccttt atcgtccatc agccgtaacg cctgtgttgg gcagacgtca atacaggctt
    24421 gcgtgccaga actgcgctgg ttacaaaggt cgcatttctg tgcaatcgta tcgaccatct
    24481 caacgacgcc aaaggggcaa gcgattgcgc atcttttaca accaatacat ttttgctcgt
    24541 ccagttgtac gctatcggac tggaaagtca gagcattaac cggacaagcc gtaacgcaag
    24601 gggcattgtt gcagtgatgg caggccaccg gattcgcagc ctggcctttc cctacaacgt
    24661 ggatacgcgg tcgaaagtca ctgtgactca gcggccagtt ttcttgatta tgtgccaccg
    24721 cacaggcaat ttcacaagca tggcagccta tacattccgc agcttcagca gcgataaact
    24781 tattcatttg catccctttc atttaatgag ttatgtcttc tcaaatttcg cgatgcaata
    24841 aacgggcata tattaggtag catgaccgtt ttaattataa ttttgcaact gagttcaaat
    24901 tattgcccct caatctatgc cgctttcatt atttcaacat tgccgatcaa aagaatgcta
    24961 tccagataaa aacgattaaa tgcgagagtg cgacatgcca gaatgattaa tgaaatatca
    25021 atatgagaaa attacagata attccattgt caggttctct tctcataatc gtggtttaac
    25081 cacgattatg tgattgcctc cgcaattcct gtctctaact ccccttcctc gcaaaaactg
    25141 gcactccacg agcatgtgtt tagacagttt catgaacata aacggttgct ttttactctg
    25201 gcgggcgaaa ggagaaacac tgatgagcgc catagattcc caacttccct catcttctgg
    25261 gcaagaccgc ccaactgatg aggttgaccg catattatca ccaggaaagc tgatcatact
    25321 cggtctgcaa cacgtccttg tcatgtacgc aggtgcagtc gctgttcctc ttatgattgg
    25381 tgaccgactc ggcctatcaa aagaagctat tgcgatgctc atcagctcgg atctcttttg
    25441 ctgcgggatc gtcacattat tgcaatgtat cggtatcggc cgctttatgg ggatccgcct
    25501 gccggtgatt atgtcggtga ccttcgccgc tgtaacacca atgatagcca ttggtatgaa
    25561 cccggatatc ggcctgctgg ggatattcgg tgccactatc gccgcgggtt ttatcaccac
    25621 attattagcg ccacttatcg gtcgcttgat gcctttattc ccgccactgg ttaccggtgt
    25681 ggtgattact tctatcgggc ttagcatcat tcaggtgggt attgactggg ccgccggagg
    25741 taaagggaat ccgcaatatg gtaatcccgt ttatttaggt atctcctttg ccgtcttaat
    25801 ttttatcttg ctcattactc gctatgcgaa aggatttatg tccaacgtcg ccgtattact
    25861 ggggattgta tttggctttt tactttcgtg gatgatgaat gaagtcaatt tatccgggct
    25921 acatgatgct tcatggtttg cgattgtcac gccgatgtca tttggtatgc cgattttcga
    25981 tcccgtttcc attctgacca tgactgccgt gttaatcatc gtgtttatcg agtcgatggg
    26041 gatgttcctg gcactgggtg aaatagtcgg tcgtaaactc tcttcacacg atattattcg
    26101 cgggctgcgt gtcgatggcg tagggacaat gataggcgga acgtttaaca gcttccccca
    26161 cacgtcattt tcacaaaacg ttggcctggt tagcgtgacg cgcgttcata gccgctgggt
    26221 gtgtatttct tcgggaatta tattaatcct gtttggcatg gtgccaaaaa tggcggtgct
    26281 ggtcgcctcc attccgcaat ttgtgctggg cggtgctggg ctggtgatgt tcggcatggt
    26341 actggcgaca gggattcgaa ttctgtcgcg ctgtaactac accaccaacc gttacaacct
    26401 ctatattgtg gcgatcagtc tcggcgttgg catgactccg acgctctctc acgatttctt
    26461 ttctaagtta ccggccgtac tgcaaccgtt gctgcatagc ggcattatgc tcgcaaccct
    26521 tagcgccgtt gtgctgaacg tcttctttaa tggctatcag catcatgctg atctggtgaa
    26581 ggaatccgtc tctgataaag atttaaaagt caggacagta cgtatgtggc ttctgatgcg
    26641 caagctgaag aaaaatgagc atggagaata atatgaatct tttaatgcgc gctatattca
    26701 gtctgctgtt gctttttact ctctctattc ctgtcatttc tgactgtgtt gcaatggcca
    26761 ttgaaagtcg cttcaaatat atgatgctgc ttttttaaat ggtttttacc tgtcgacatc
    26821 cgctcaaaac gggcggttgt cgataaacgc tcacttggtt aatcatttca ctcttcaatt
    26881 atctataatg atgagtgatt agaattacat gtgagaaatt atgcaaacgg aacacgtcat
    26941 tttattgaat gcacagggag ttcccacggg tacgctggaa aagtatgccg cacacacggc
    27001 agacaccctc ttacatctcg cgttctccag ttggctgttt aatgccaaag ggcaattatt
    27061 agttacccgc cgcgccctta gcaaaaaagc atggcctggc gtgtggacta actcggtttg
    27121 tgggcaccca caaccgggag aaagcaacga agacgcggtg atccgccgtt gccgttatga
    27181 gcttggcgtg gaaattacgc ctcctgaatc tatctatcct gactttcgct atcgcgccac
    27241 cgatccgaat ggcattgtgg aaaatgaagt gtgtccggta tttgccgcac gcaccaccag
    27301 tgcgttacag atcaacgatg atgaagtgat ggattatcaa tggtgtgatt tagcagatgt
    27361 attacgcggt attgatgcca cgccgtgggc gttcagtccg tggatggtga tgcaggcagc
    27421 caatagtgaa gcaagaaaat tgttgtctgc tttcgcgcag cacaattaaa aaaccccgac
    27481 atttgccggg gttatgagca taacgtaatg cttattttac cggacgcatc gccgggaaca
    27541 gaataacgtc gcggatggta tggctgttgg tgaacagcat taccatacgg tcgataccaa
    27601 ttcccagacc tgctgtcggc ggtaagccat gttccagtgc ggtgacgtaa tcttcatcgt
    27661 agaacatcgc ttcgtcgtcg cctgcatctt tcgcggcaac ctgatccagg aagcgctgcg
    27721 cctgatcttc cgcgtcattc agttcgctaa agccgttacc gatttcacga ccaccaatga
    27781 agaactcaaa gcggtctgtg atttccgggt taacgtcgtt acgacgcgcc agcggagaaa
    27841 cttctgccgg atattcagta atgaaggtcg gctgaatcag atgtgcttct gccacttctt
    27901 cgaagatctc ggtaacgata cggcccagac cccagctctt ctcaacgtgg ataccgatag
    27961 attcagcaat cgctttcgca gagtcgaagt tgtccagatc cgccatgtcg gtttccgggc
    28021 gatatttctt gatcgcttca cgcatggtca gtttttcgaa cggtttaccg aagtccagcg
    28081 tcacgtcgcc gtaggtcact tccgtcttac cgagaatatc ctgtgccaga gtacggaaca
    28141 gcgattcggt cagctcgatc agatctttgt aatctgcgta agccatatag agttccatca
    28201 tggtgaactc tgggttatga cgtacggaaa taccttcgtt acggaagtta cggttgattt
    28261 cgaatacacg ctcgaagcca ccgaccacca gacgcttgag gtacagttcc ggcgcgatac
    28321 gcaggtacat gtcgagatcc agcgcgttat ggtgagtgat aaacggacgc gcagcggcac
    28381 cgccagggat cacctgcatc atcggcgttt caacttccat aaagccgcgg ttcaccatga
    28441 actggcgaat accagagagg atctgcgagc gcactttaaa ggtgttgcgg gattcatcgt
    28501 tagagatgag atccagataa cgctgacgat agcgcgcttc ctggtcctgc aagccgtgga
    28561 atttatccgg cagcggacgc agtgctttgg tcagcagacg cagctcggta cagtggatag
    28621 acagttcgcc agttttggtt ttgaacagtt tacctttcgc gccgaggata tcgccgaggt
    28681 cccatttttt gaactgctcg ttgtaaacgc cttccggcag atcgtcacgg gcaacgtaca
    28741 gctgaatgcg gccacccaca tcctgcaggg taacgaaaga cgctttaccc ataatacggc
    28801 gggtcatcat gcggccagca acggcgactt cgatgttcag cgcttccagt tcttcgttct
    28861 ctttaccgtc gaattctgcg tgcaattggt cagaggtatg atcgcgacgg aaatcgttcg
    28921 ggaaggcaat cccctgctcg cgcaggttcg ccagcttctc acgacgcgtt ttcagttcat
    28981 tgttaagatc gactaccgcg tcagcgccct gtgcgtgttg ttcagacatg ttggttcctc
    29041 ataaccctgc tttcaaactt gcttcgataa attgatccag gctgccgtcc agcacggcct
    29101 gcgtgttacg ggtttctacc ccggtacgca gatctttaat gcgggagtca tcaaggacat
    29161 aagaacgaat ctggctgccc cagccgatgt cggatttgtt atcttccatc gcctgtttct
    29221 cggcattttt cttctgcatc tccagttcat aaagcttcgc tttcatctgc ttcatggcct
    29281 ggtctttgtt cttgtgctgg gaacggtcgt tctggcattg ggtcacgatc ccggttggga
    29341 tgtgagtaat acgcaccgca gattcggtac ggttaacgtg ctgaccgccc gcgccggatg
    29401 cgcgataaac gtcgatgcgc agatccgccg gattgatttc gatatcgata tcatcatcaa
    29461 cttccgggta aacaaacgcg gagctgaacg acgtgtggcg acgaccgccg gagtcaaacg
    29521 ggcttttacg caccaggcgg tgaacgccgg tttctgtacg cagccagcca taagcgtaat
    29581 cgccggagat tttgatcgtc acggatttaa tacccgccac ttcaccttcc gactcttcga
    29641 tgatttcagt tttgaaacca cgcgattcag cccagcgcag atacatacgc tcaagcatgc
    29701 tcgcccagtc ctgtgcttcc gtaccgccag accccgcctg aatatcgagg tagcagtcgg
    29761 cgctgtcata ttcgccagag aacatacggc ggaactcaag ctgcgccagt ttttcttcca
    29821 gggcgtcgag ttcagcaacg gcttcgttaa aggtttcttc gtcgtcagct tctacagcca
    29881 gttccagcag accagaaaca tcttccagcc cctgtttcat ttggtcgagg gtgtcgacaa
    29941 cggcttcgag ggaggaacgc tctttaccca gcgcctgtgc gcgttcgggt tcgttccaga
    30001 catccggctg ttccagctcg gcgtttactt cttccagacg ctctttcttg gcgtcatagt
    30061 caaagatacc ccctaagaac gtcggagcgt tccgtgaggt cctgaatgcg attatttacc
    30121 ggattaattt caaacatggt ctgatttctt ttattgagct agtcaaaatg cggtgataag
    30181 agcgggattg tacccaatcc acgctctttt ttatagagaa gatgacgcta aattggccag
    30241 atattgtcga tgataatttg caggctgcgg ttgccgcgaa actcgttgat atcgagcttg
    30301 taagccagtt gcacttcgcg cacaccgtta tccggccaga gggcggtatc gacattaaaa
    30361 gcaatcccgt ccagcagcgg accgccgccg accggttcga ccatcacctt caaatgacgc
    30421 tcgcccacca gccgctgttg caacagacgg aaatgaccat caaacagcgg ttccgggaac
    30481 atttgtcccc acgggccagc atcacgcaac agctgcgcca cttccatggt catctctgcc
    30541 gcacttaatg ggccgtctga tataacttcc ccttgcaata aagaaggatc taaccactcg
    30601 gtaaccagtt cgccaaagcg ttgctgaaag agttcgaatt tatcctcttc cagcgacaat
    30661 cctgccgcca tcgcatggcc gccaaacttg agcatcatgc cggggtaaag cgtgtctagc
    30721 cgctccagcg catcgcgcat atgcagcccc tgaatggagc gaccggaacc tttcagcgta
    30781 ccgtcacccg caggcgcaaa ggcgataacc ggacgatgaa aacgctcttt gatgcgcgaa
    30841 gccagaatac cgacaacgcc ctgatgccat tcggggtgat acattgccag cccgccgggt
    30901 agcgtgtcgc ggctgcgctc cagtttctcg cacaaggtca gggcttcaac ttgcattccc
    30961 tgttcgatct cttttcgcgt ctggtttagc gcatcgagtt catttgccag cacgcgcgct
    31021 tcgccgatgt tgtcgcacag caacagcgcc acgccaacgg acatatcgtc cagtcgcccg
    31081 gcagcattga gacgtggccc cagcgcaaaa cctagatcgc tggcggcgag tttttgtgca
    31141 tcacggtttg ccacttccag cagcgcttta atccccggac ggcactttcc ggcacggatg
    31201 cgactcatcc cctgccaggt cagaatgcga ttattagcgt ccagcggcac gacgtccgcc
    31261 actgtcccca acgctaccag atccagcagt tctgccagat taggaattgc gatgccgcgt
    31321 tcatcaaacc agccctgatc gcgcaaaaaa gtacgtaacg ccagcatcag ataaaacgcc
    31381 acgccaacgc ccgccagtga tttcgacggg aaattacagt cgcgcaagtt agggttaatg
    31441 atcgcttccg ctgcgggtaa tgtgtcgcct ggcaaatggt gatcggtaac aataaccgga
    31501 atgcccaacg aacgagcgtg ttcaaccccc gcatgggagg aaataccgtt atccaccgtg
    31561 acaattaact gcgcgccacg ggcatgagcc tgatcgacta cttccgggct taagccgtaa
    31621 ccgtcttcga aacggtttgg aaccaggtag tcgatattgc tgcaaccaag cgagcgcatt
    31681 gccagcacgc ttagagccgt gctggtcgca ccgtcggcgt cgaaatcacc gaccacaata
    31741 atccgcgttc cttcgcgaaa agcgttgtaa aggatctcaa cggccttttc gacgccgctc
    31801 agttgctgcc agggcagcat gcctttaaca ctgcgttcca gttcctgcgc gctacgcact
    31861 ccccggctgg cgtataaacg gcgcagcaag ggaggcaatt ccgcgggcaa gtcggccgtt
    31921 tcatcgactt cacggcgacg aagttgtatc tgttgtttca cgcgaattat ttaccgctgg
    31981 tcattttttg gtgttcgtcg aggaattctt tcatgtcttt cggcggctgg taacccggaa
    32041 caagtgtgcc attgctcagc acaactgccg gagtaccgct aacgccaagc tggacgccaa
    32101 gtgcgtaatg gtcggcaata tccacgtcgc agctggctgg tgcgacgctt ttacctgcca
    32161 tcacatcatc aaacgctttg tttttatctt tcgcacacca gatagctttc atttctttct
    32221 ctgcatcgct gtccagcccc tggcgcggga aagcaagata acgcacggtg atccccagcg
    32281 cgttatagtc tgccatttgc tcatgcagtt tgtggcagta accacaggta atatcagtaa
    32341 acacggtgat gacgtgtttt tcctgcggcg ctttataaac gatcatctct ttttcaagcg
    32401 cattcaactg ctttaacagc atcttattgg tgacattgac cggagccgtg ccactaacgt
    32461 catacattgg cccctgaatg atatgtttac catcatcggt gatgtacaac acaccgctgt
    32521 tagtcagaac tgtcttcatg ccagctacgg gcgcgggctg aatatcgctg cttttgatgc
    32581 ccattttggc taacgtttgt tgaattgccg cgtcatcagc ctgaacaaag cctgaaaacg
    32641 ctgccaacaa ggtaaataac ataaaacctt tcttcataaa ttttcctgtt cttttcagac
    32701 atcacgcccg cgggtgatgc tgttgatgaa gttgccgcag acgctcggta gcgacatgcg
    32761 tataaatttg cgtggtggag agatcgctgt ggcccagtag catctgcacc acgcgcaaat
    32821 ccgcaccatg atttaataag tgagtggcaa aagcgtgacg caacacatgc ggtgacagct
    32881 tttcgctgtc gatacccgcc agcacggcat aatgtttaat gcggtgccag aaggtctgtc
    32941 gcgtcatctg ctgcgcacgc tggctgggaa acaacacgtc aattgacaca ccattcaaca
    33001 gccacggacg cccatgttcc agataggttt ccagccagta aaccgcctct tcacctaacg
    33061 gcaccaggcg ctctttgttg cctttaccaa tgacccgcac tacgccctga cgcaggctga
    33121 tatcactcat tgtcaacccg accagttcag agacacgcag tccggtagca tacaacactt
    33181 caagcatggc tttatcgcgt agctccagtg gctgatcaat taatggtgcc tgtaataaac
    33241 gttcgacctg cgcttcgctt aaatcttttg gcaaacgctg gggcaatttc ggtgaagcga
    33301 gatgcgcact gggatcatct tcacgaaact tttcgcgata aagatactgg aacaatcggc
    33361 gcactgcact cagcaaacgc gctgagctgg tggctttata cccgccctcc agccgttctg
    33421 ccagtaatgc ctgcaaatcg tcactttgcg ccgtcgccag cgtcaacccg cggtgatgca
    33481 accactccac catcattgac agatcgcgac ggtaagcgtt caacgtattt tcagccagat
    33541 ttttttccag ccacagagca tcaagaaact gctcgatgcg tgccagatcc tgtttcactt
    33601 gcgcccctta tggtcactca tttgatccat tatgccttat tgtgccgtga ctaaagcgat
    33661 tctgatacac tagccgcaaa agccacagca gaatcgagaa gcttacgtta tgaatatggg
    33721 tcttttttac ggttccagca cctgttacac cgaaatggcg gcagaaaaaa tccgcgatat
    33781 tatcggccca gaactggtga ccttacataa cctcaaggac gactccccga aattaatgga
    33841 gcagtacgat gtgctcattc tgggtatccc gacctgggat tttggcgaaa tccaggaaga
    33901 ctgggaagcc gtctgggatc agctcgacga cctgaacctc gaaggtaaaa ttgttgcgct
    33961 gtatgggctt ggcgatcaac tgggatacgg cgagtggttc ctcgatgcgc tcggtatgct
    34021 gcatgacaaa ctctcgacca aaggcgtgaa gttcgtcggc tactggccaa cggaaggata
    34081 tgaatttacc agcccgaaac cggtgattgc tgacgggcaa ctgttcgtgg gtctggcgct
    34141 ggatgaaact aaccagtatg accttagcga cgagcgtatt cagagctggt gcgagcaaat
    34201 cctcaacgaa atggcagagc attacgcctg atgtcactga cggcttagca catatgcttt
    34261 gccgtcatct ttatctttgc gtctcttgtt gtaacaatat ccgccgtaaa tcccgccatt
    34321 cggcttcgtc catgctgtca gctgccagcc ataaatgttg ccgttttccg ctgtcagaac
    34381 gtaaacgcag catcatgccg ctcttaatca tccacggcgc tttgacgatg ctccactcct
    34441 gcccttgcca acgcaaacgc ccgtccatca acaggcgaat ttccccctgg cgagcattaa
    34501 tacgccgctg gctgcgaacg caatcaaata ccaccagcga aagtaacacc atccataacg
    34561 gggtgtaact gagcggccag ggcatgagta aaataacagc ggcaaccagc ccatgaatca
    34621 gcaaggaaag ccactgtgcg cgccaggaga cgcgcaaatc agattgccac aggaccacgt
    34681 tcccggttcc gtgtctggat gagtcggacc atcatttcca gttctgcatc ggccggttta
    34741 ccgtgattca tcagccagtt aaacaggtcc ggatcgtcac attccagcag acgaataaag
    34801 atgcgttttt cgtcatcgct taagctgtcg tactcatgtt cgaaaaacgg catgattgaa
    34861 atatcgagtt cgcgcatacc acggcggcat gcccaatgaa tgcgggcttt gttgttaatg
    34921 tccatcttct tcctgtctca cgaaaatcca gtaccccgct attgtaacgt gtttttcgac
    34981 ttcttttgcg ggaatatcag taaacacaat cgcgatcgcg aaattaattc acaacaattc
    35041 aagggcttca tttttttgga agtcgcctcg cagaaggcac agatcgcgta gtgaaagcac
    35101 ttgcattgcc tcatagctct tttaccatta gtgattaata cgccgttaag caactcagga
    35161 ctctattatg gcttttacac cttttcctcc ccgtcagcct acggcttctg cccgtttgcc
    35221 actgacgctg atgacgcttg atgactgggc gcttgccacc attactggcg cggacagcga
    35281 aaaatatatg cagggtcaag tgacagcaga tgtcagccag atgacagaag atcagcacct
    35341 gctcgccgcc cattgcgacg ccaaaggcaa aatgtggagc aacttacgtc tgttccgcga
    35401 cggcgatggc tttgcatgga ttgaacggcg cagcgtgcgt gaaccgcagc tgactgaact
    35461 gaaaaaatat gcggtgttct ctaaagtgac catcgcgcca gacgacgagc gtgtgctgct
    35521 tggtgttgcc ggttttcagg cgcgcgccgc gttggcaaat ctctttagtg aactaccttc
    35581 gagagaaaaa caggtagtca aagaaggcgc gaccacgttg ctatggtttg aacacccggc
    35641 agaacgtttc ctgatcgtaa ccgatgaagc tactgccaat atgctgaccg ataaactgcg
    35701 cggtgaagcg gaactgaaca atagccaaca gtggctggca ttaaacattg aagcgggttt
    35761 cccggtgatt gacgccgcca acagcgggca gttcattccg caggcgacca acctccaggc
    35821 gctgggcggc atcagcttta aaaaaggctg ctataccgga caagagatgg tggcacgggc
    35881 taaattccgc ggtgccaaca aacgcgccct ctggctgctg gcaggtagcg ccagccgact
    35941 gccggaagcg ggtgaagatt tagagctgaa aatgggcgag aactggcgtc gtaccggtac
    36001 ggtgctggct gcggtaaaac tggaagatgg tcaggtcgtg gtacaggtcg tcatgaataa
    36061 cgatatggaa ccggacagca tcttccgcgt gcgtgacgat gcgaatacat tgcatatcga
    36121 gccgctgccc tattcgctcg aagagtaaat ctctgtatcg catcaggcac ttatcgcctg
    36181 atgcgacgct gacgcgtctt atcatgccta caacggcgca ccaattacgc ctgcccaata
    36241 tacaaataga tcgccagaaa gtggcacacg ctgccgccga gcacaaagcc gtgccagatg
    36301 gcatggttgt atgggatgcg cttgcagacg tagaaaatca ccccgagcga gtagaccacg
    36361 ccgcctactg ccagtaaggt aacgctgccc gccgcgagct taactgccat ttcataaatt
    36421 accaccagcg acagccagcc catcgccaga taggtcacca gagataaaat tttgaatcgg
    36481 tgcgcgatgg tcagtttaaa cagaataccc agcaatgcca ggctccagat aacaatcatc
    36541 aacccgcgcg ctaacggaga atccagcccc accagcaaaa acggcgtata ggttccggca
    36601 atcaacagat aaatggcaca gtggtcaaat ttcttcagcc acatttttgc ccgttgatgg
    36661 ggaatggcgt gatagagcgt cgaagcgagg aacagcagga tcatactgcc gccatagagg
    36721 ctgtaactgg ttatcgccgt ggcgctggca ttgagatcca ccgcctgaac cagtagcaac
    36781 accagcccaa cgataccaaa caccagccca atgccgtgac tgacgctgtt ggcaatttcc
    36841 tctgccagcg aatatccctg cttaatgagg ggcttctgaa ccataactta ctccggaaaa
    36901 acgtacacgc acatgtatac ctctccagcg taactgagaa tagttccagt gaacacctgt
    36961 tagctaaaat aaattctgat ttgagattaa tctccgaaaa atcatgaaat taaatgcgaa
    37021 atttcaacta acaggcgtga gttcaattta aagacattta aattcaatca cataaaactg
    37081 tgtctggtcg ggatagatgt cggcaatgac ctttttcagt tcagtcaggg tcatattttc
    37141 ctgctctgca tgtttttccg tcagcgtatc cagcgttacg gttgaggttg cggtaacttc
    37201 aatcgtgcaa aaataaccgt catcttcaaa ccgtccgaca cgaagcacat cacccgtttt
    37261 gaagtgcgat tcagactcgt cgcggatggt gatggtttta cgcccagcca gaatgtcatc
    37321 ctggaaacgt tgaaaaaaag tgatgtcgtt tggctgcatg gtaatatttc ctgtaagaat
    37381 tgactcatct ggagcctatg atagtgaaaa aactcacctt accgaaagat ttcttatggg
    37441 gcggcgcagt tgccgctcat caggtcgaag gcggctggaa caaaggcggc aaagggccga
    37501 gcatttgtga cgttttgacc ggtggcgcac acggcgtgcc gcgcgaaatc accaaagaag
    37561 ttgtgcctgg caaatactat ccaaaccatg aagccgttga tttttatggt cactacaagg
    37621 aggacatcaa gctatttgcc gaaatgggct tcaaatgttt tcgtacttcc atcgcctgga
    37681 cgcgcatttt tccaaaaggc gatgaagctc agccaaacga agaagggctg aagttctacg
    37741 atgatatgat cgatgaactg ctgaaataca acatcgaacc ggtgatcacc ctctcccact
    37801 ttgaaatgcc gctgcatctg gtgcagcaat atggtagctg gaccaaccgt aaagtggttg
    37861 atttctttgt ccgtttcgcg gaagtggtat ttgaacgcta taagcataaa gtcaaatact
    37921 ggatgacctt caacgaaatt aacaaccagc gtaactggcg tgcaccgctg ttcggttact
    37981 gctgctccgg cgtggtgtat accgagcatg aaaacccgga agagacgatg tatcaggtgc
    38041 tgcatcacca gtttgtcgcc agcgccctgg cggtgaaagc cgcgcatcgc atcaacccgg
    38101 agatgaaagt cggttgtatg ctggcgatgg tgccgctcta tccttactcc tgtaacccgg
    38161 acgatgtgat gtttgcccag gagtcgatgc gcgaacgtta cgtctttacc gatgtgcagt
    38221 tgcgtggcta ttacccgtcc tatgtgttga acgagtggga acgccgcgga tttaacatca
    38281 aaatggaaga cggcgatctg gatgtactgc gcgaaggcac ctgcgattat ctcggcttca
    38341 gctattacat gaccaacgcg gtgaaggccg aaggcggcac cggcgatgcg atctctggtt
    38401 ttgaaggcag cgtaccgaac ccgtatgtta aagcgtctga ctggggctgg cagattgatc
    38461 cggttggtct gcgttacgcg ctttgcgagc tgtatgagcg ttaccagaag ccgctgttta
    38521 ttgtcgaaaa cggttttggc gcttacgaca aagtggaaga tgatggcagc atcaatgacg
    38581 attaccgcat tgactacctg cgcgcccata ttgaagagat gaaaaaagcg gtgacttacg
    38641 atggcgtgga cctgatgggc tacacgccgt ggggctgcat cgactgtgtg tcgttcacca
    38701 ccgggcagta cagcaaacgc tacggcttta tctatgtgaa taaacatgac gacggtactg
    38761 gcgatatgtc gcgttcacgt aagaagagct ttgactggta caaagaggtg attgccagca
    38821 acggcgagaa tctgtaatta gccgatgtgc ctgatgtgct ccgctcatca ggcctggcac
    38881 tcttttaata tattgagctc gcaatatttt atattacgcc tcagccgaaa cacacaaagc
    38941 gcactttatt aacaagcgaa aaaggcgccg aagcgccttt ataagatagt cgaatcagta
    39001 aattactggt attcgctaat cggtacgcag gagcagaaca ggttacggtc gccgtaaaca
    39061 tcatccagac gtttcacggt cggccagtat ttgtctgcca cacctgccgg gaatactgca
    39121 acttcacggc tgtacggatg cgcccactcg gcgaccagtt cgctctgaat gtgcggcgcg
    39181 ttcaccagcg ggttatcttc cagcggccag acaccggctt tcacctggtc aatttctgcg
    39241 cggatagcca gcatcgcatc gataaagcga tccagttcca ctttgctttc agattcagtc
    39301 ggttcaacca tcagcgtacc cgccaccggg aacgacatgg tcggcgcgtg gaaaccgtag
    39361 tcgatcaggc gcttggcaat atccagctcg ctgatgccgg tttcttcttt cagcgggcga
    39421 atatcgagaa tacattcgtg cgccacgcga ccgtcgcgac cggtatacag cacagggaag
    39481 gcatcctgca gacggctggc aatatagttg gcgttaagaa ttgccacctg gcttgctttt
    39541 ttcagccctt ctgcgcccat catgcggatg tacatccagc tgattggcag gatagaggca
    39601 ctaccgaacg gtgccgcaga aaccgcgccc tgacgggtta acatgccttc gatttgcacc
    39661 acgctatgac ccggtacaaa cggagccaga tgcgctttca caccgatcgg tcccataccc
    39721 ggaccaccac cgccatgcgg aatgcagaaa gttttatgca ggttaaggtg ggaaacgtcc
    39781 gcaccaataa agcccggcga ggtgatgcca acctgagcgt tcatgttcgc gccatcaagg
    39841 taaacctgac cgccgaactg atgcacgact tcacacactt cacggatcgt ttcttcatac
    39901 acgccgtggg tagaaggata ggtcaccatg atacaggaga ggttatcgcc cgcctgttcc
    39961 gctttcgcgc gcagatcagt cagatcgatg ttgccgtttt tatcacacgc cacaaccacc
    40021 acctgcattc ctgccatatg cgcggaagcc gggttagtgc cgtgcgcaga agccgggatc
    40081 aggcagatat cgcgatgccc ttcgttgcgg ctttcatgat aatgacgaat cgccagcagg
    40141 cccgcgtatt cgccctgtgc gccagagttc ggctgcatac aaacggcgtc gtaaccggtc
    40201 agtttcacca gccagtcagc cagctgcgcg atcatctgct gataaccttc agcctgttcc
    40261 ggcgggcaga acgggtgcag ttcggcaaat tccggccagg tgatcgggat catctcggcg
    40321 gcggcgttca gtttcatggt gcaggaaccc agcgggatca tcgcctgatt cagcgccaga
    40381 tctttacgct ccagcgagtg catatagcgc atcatttcgg tttcgctgtg gtagcgatta
    40441 aacaccggat gggtgaggat ttcgtcgtcg cgcagcatcg cagcctgaat agagcgactg
    40501 tcgtgagcca cgtctttgtc cagtgtgtcg atgtccagcc cgtggttatc gcccaacagc
    40561 acgctgaaaa gctgcatcac gttttcgcgc gtggttgttt catcaagggt gatcccaacc
    40621 gcgttcagaa tatcgctacg caggttgatt tcagctgctt cggcacgcgc cagcacgccc
    40681 gctttgtcga ccacttccac acacaaagta tcgaaatagt gcgcatggcg cagcttcaga
    40741 cctttttgtt gcaggcccgc cgccaggata tcggtcagac ggtggatgcg gttagcgata
    40801 cgtttcaggc caaccgggcc gtgataaacg gcatacaggc tggcgatgtt tgccagcagt
    40861 acctgggaag tacaaatgtt ggagttcgct ttctcacgac gaatatgttg ctcgcgagtc
    40921 tgcatcgcca tgcgcagagc ggtgttgcca gctgcatctt tcgatacacc gataatacgg
    40981 cccggcattg agcgtttgta ttcgtctttc gccgcaaaga atgccgcgtg tgggccacca
    41041 tagcccatcg gtacgccaaa gcgttgcgcc gaaccaaaaa caatatccgc gccctgttta
    41101 cccggcgcag ttaacagcac cagcgccata atatcggcgg caacgctgac cacgattttg
    41161 cgtgatttca gttcgctgat aagcgcggtg tagtcgtgaa tttcaccagt agtgccgacc
    41221 tgctgtaaca gcacgccgaa gacgtcctga tggtcgagca ctttttgcgc gtcatcgaca
    41281 atcacttcaa aaccaaaggt ttcggcacga gtacggacca catccagcgt ttgcggatga
    41341 acgtcagaag caacaaagaa gcggttggca tttttcagtt tactgacgcg tttcgccatc
    41401 gccattgctt cggcggcagc ggtggcctca tccagaagag aagccgaagc catatccagc
    41461 ccggtcaaat ccagcgttac ctgctggaag ttgagtagtg cttcaaggcg gccctgggag
    41521 acttcaggtt gatacggagt atacgcggta taccagcccg gattttccag catgttacgc
    41581 aggataaccg gcggtagctg cacggcggtg taacccatgc cgatgtaaga cgtgaagcgt
    41641 ttattgcgac tggcaatagc cttgagttct gccagtgcgg cgtattcggt cgccggtgcg
    41701 ccaacctgcg gtggtgtcgc aagctgaata tctttcggca caatctggcc ggtcagcgcg
    41761 tttaacgatt gtgcaccaac ggcattcagc atttcttgct gttgcgcggc gtccggtccg
    41821 atatggcgtt caataaaagc gccgctgttt tcaagctggc ttaacgtctg tgtcatgagc
    41881 gatggttcct gaaacgtgca gtgaattgtg aacctctctc cttgcgaaga gagtgagggt
    41941 gaggcgtaaa tttcctcacc ctgatcctct cccgcagaag aggaataaag ccgttactcg
    42001 tcttctaaca atgcttcgta tgcggttgca tccagcagtg attccagttc gctttcatcg
    42061 ctggctttga ttttgaaaat ccagccgcct gcatacggtt cgctgttcac cagttccgga
    42121 gaatcgctca gtgcatcgtt taccgccacg atttcaccgc ttactggcgc ataaatgtct
    42181 gacgccgctt ttaccgattc ggcaaccgcg cagtcatcgc ccgcgctaac cgttgcgccc
    42241 acttccggta ggtcaacaaa caccatatcg cctaacagct cctgagcgtg ttcggtgatg
    42301 ccaacagtgt aagtgccgtc ggcttcttta cgcaaccatt cgtgttcttt gctgtatttc
    42361 agttctgctg gtatgttgct catcaatcaa tctccaaaaa agtaaatcac gcgacggctt
    42421 tgccgttacg cacaaaaaca ggtttcgtca ctttaaccgg catttcacgg ttgcgaattt
    42481 gcacaatcgc cgtttcacca ataccttccg gcacgcgcgc cagcgcaatg ctataaccca
    42541 gcgttgggga gaaagtaccg ctggtgataa tgccttcatg ctggttgccc tgcgcatcag
    42601 taaaacgtac cggcagttca ttacgcagca cgcctttttc ggtcatcacc agaccaacca
    42661 gtttttctgt gccctgttca cgctgcgctt ccagagcttc acgaccgata aagtcacgat
    42721 ctgccggttc ccaggcgata gtccagccca tattggcggc taaaggagaa atagtttcgt
    42781 ccatctcctg gctatacaaa ttcatgcccg cttccagacg cagcgtgtca cgcgcgccca
    42841 ggccacatgg cttaacgcca gcttccacca gcgcgcgcca gaaatcggcc gctttttcat
    42901 tgggcagcgc aatttcgtag cccgcttcac cggtgtaacc ggtggtggca ataaacagat
    42961 cgcccgcctg cacgccaaag aacggtttca tcccttccac cgcctgacgc tgggcgtcat
    43021 taaacagtgt ggcagctttt gcctgcgcat tcggcccttg tacggcgatc atggaaaggt
    43081 catcacgaac agtaatttcg atgccgaaag gttcagcgtg ttgggtaatc caggagagat
    43141 ctttttcgcg ggtggcggag ttaacaacga ggcggaagaa atcttcagta aagtagtaga
    43201 cgatgagatc atctatcact ccgccagagg cattcaacat ccccgagtaa agggctttgc
    43261 cgcttttggt gagcttcgcc acatcgttcg ccagcagata acgtagaaac tcccgggtgc
    43321 ggctgccgcg aagatcgacg atggtcatat gtgacacatc aaacattccg gcttcggtac
    43381 gtaccgcatg atgttcgtcg atttgcgaac cgtaatgcag cggcatcatc cagccgtgga
    43441 aatccaccat gcgagcgccg caaagcgtgt gttgttcgta caaaggagtc tgttgtgcca
    43501 tcttgtcctc attgaataag cggggctgac aacttttcca tggcgaaatt atcaccacga
    43561 aacccagcat cggagccact cccggtcccc aacgcaatcg ttctcttttg cctgaactta
    43621 ccaccgaaac agactgttaa ccataaggta aaattgatca tcacattagc ttatggttaa
    43681 aaaatgccaa aatcgcaaca gaataaaaaa ccaaaaaaca catcagtttc tatacaaaga
    43741 tgatgtgatg agaaatttaa tttgaataag acaatattaa gcgctaaaaa aatgtcaaaa
    43801 aacactaaat caaaaatcca taacattaga aaatataatg cgaaaacgga ggttaaatta
    43861 gtttatttca aatgaggaaa atctcccggc gaaaaaaccg ggagatgaaa gtgtgatggg
    43921 tatcaaataa acagcagaag agaaattttt aacgcagcca ttcaggcaaa tcgtttaatc
    43981 ccattgcctg gcggataagt tgtggtttaa cgccaggaag cgtgtcggcc agtttcaaac
    44041 caatatcacg cagcagtttt ttcgccggat tagtaccgga aaacagatcg cgaaatccct
    44101 gcataccagc cagcatcagc gccgcactgt gcttacggct acgctcatag cgacgcaggt
    44161 aaatgtactg cccgatgtct ttaccctgac gatgcaaccg tttcagttcg gcaatcagct
    44221 ctgcagcatc cataaagccg agatttaccc cctgccccgc cagcgggtga atggtatgcg
    44281 cggcgtcgcc caccagcgcc agacggtgcg cggcaaactg gcgcgcataa cgccccgtca
    44341 gtgggaacac ctgacgcgcg ctctcaacct tgcataagcc caggcgatta tcaaaagcga
    44401 tatttaacgc gcgattaaat tcgtcttcac ttgcctgctg catccgctgc gcttcctctg
    44461 gcgacagtga ccagacaatc gagcaaagat gcggatcgct aagcggtaaa aaggccagaa
    44521 tgccttcgcc atggaaaacc tgccgcgcca ccgcatcatg cggttcttcc gtgcgaatgg
    44581 tcgctaccag cgcgtgatgc tgataatccc agaaagtcag cggaatatcg gctttgttgc
    44641 gcaaccagga attagcgccg tccgcgccaa tcaccagacg cgccgttaac atgctgccat
    44701 ctttcagcgt caggaaggtt tcattttctc cccaggcgac ctgctgtaat tctgcggggg
    44761 ccaacagatt gatatctgac gactgctgcg ctttgttcca tagcgcgtag tgaatcactg
    44821 agttttcaac gatatgccca agatgactat agcccatgct ttgatcgtca aacgaaatgt
    44881 gaccaaaact gtctttgtcc cacacttcca taccgtgata acaactggcc ctacgagaga
    44941 gaatgtcctg ccagacgcca agacgggtga gtaatttttc gctggcggca ttgatagccg
    45001 aaacgcgcag ttgtggtggt gcatccgccg ccagaggttc cggtacgcgc tgctccagta
    45061 cggcaacgcg taagccgctc ccctgtaagc cgcaggcaac cgccagcccc accatgccgc
    45121 caccaacaat ggctacatca acactttgca ttgtttattc cttaaaaccg ccttcaacgc
    45181 gccacccaac cgagggtgcg ctgcgccagc acatcgcgtg ccggggtaaa taattccatc
    45241 gtcatcagcc cgatgttgcg cccgacaacc agcggggtcc aacggttggc aaaaagatgt
    45301 acaaggctgt ccgtgacgcc gatggttgct tcgcgatcgc tctgtcgacg ctgctgataa
    45361 cggcacaata cgccgtaatc ccccatgtct tctccgcgct cctgcgcctg agtcagggtt
    45421 tctgcaaggc tcatcacatc acgcataccg aggttgaacc cttgcccggc gatggggtgc
    45481 agagtttgcg ccgcattgcc caccagcaca gtgcgatggg taatcggtct ggcggcgcgg
    45541 gttaatgcca gcggataagc actgcgttta ccggcgtggg taatttgccc cagtcgccag
    45601 ccaaaggcag actggagttc acggcaaaac ttctcgtcgc tccacgacag cacctcttcg
    45661 cgccgttcca gtggatgaca ccagaccagc gaacagcgtc cgtcagacat cggcaacatc
    45721 gccagcgggc catgtttcgt gaagcgttca aaagcgcggc cttcgtgcgc aacggaagtc
    45781 gcaacgttag caatcacagc cagttgttcg taaggctcct gctgccagtc aacgccgcac
    45841 acagtggcta acgctgaatg ggtgccatca gcggctacca gcacgcggcc cgtcagcgtc
    45901 tcaccactct ccagcgtcac ttcaacgtgg ctctgagtac gggcaacgtt agccacgcga
    45961 tcagggcaat gcagcgttac gcctggtgct ttacgcaaca gcgcaaacag ccgttgccca
    46021 acattgtgca attcgacaac ctgccctagc gccgccagtt ggtaatcttc tgcggcaagg
    46081 gtgacaaatc cggcatgacc acgatcgctg acatgcactg tggtgatggc agttgcgcaa
    46141 tcagccagag attgccagac gccgatgcgc gccagttgct gacaggtacc cgccgccagc
    46201 gcaatagctc gtccatcaaa gcccggatgc gcatgtgact ctggcgcggt cgcttcaatc
    46261 aaatgtactg acagcgcccc gtgacttaac cgggaaatag ccagcgccag cgtcgcgccc
    46321 gccatgccgc caccgacgat gattacactc attgctttct cgcagctgcc atcaacgctt
    46381 cgatttcttc cggctttttc accacgctgg cggtgaggtt ttcattaccg gtttcggtaa
    46441 tcacaatatc gtcttcaata cgaatgccga taccgcgata ctgttctggc acttctgcat
    46501 ccggcgcaat atacagccct ggttctacgg tcagtaccat acccggttcc agaatacgcg
    46561 agcgatcctg accataaaca ccaacgtcat gaacatccag ccctaaccag tggctaaggc
    46621 catgcataaa gaaaggacga tgggcgttct gagcgataag ttcatcaaca tcacctttca
    46681 ggatgccgag ttttaccaga ccgctaacca tgatgcgcac cacttcacca gtgacttcct
    46741 gaatggaagt tcccggacga tacaggcgca ggctggtttc aagagactcc agcacaatgt
    46801 catagatttc acgctgagcc tgagtgaatt tgccgttgac cgggaaggtg cgggtaatgt
    46861 cgccagcgta acctttgtat tcacaacccg cgtcaatcaa caccaggtca ccgtcacgca
    46921 gttcacactc gttttcggtg tagtgcaaaa tgcagccgtt ttcaccgctg ccaacaatgg
    46981 tgttatagga cgggtaacgc gcaccgtggc ggttaaattc gtggtgaatt tcgccttcca
    47041 gatggtactc gaacatcccc ggacggcatt tttccatcgc ccgtgtatgc gccagtgcgg
    47101 tgatttcacc cgcgcggcga agtacggcaa tctcttctgg tgatttgaac aggcgcattt
    47161 catgaacaac aggacgccag tcgatcatcg ttgccggtgc ggtgagattt tgccgcgaac
    47221 ctttacgcag tttctcaagc gcgctgttca cgatttcatc agcatatgca tgttcgccct
    47281 gggcgtggta aaccacatcc agaccattaa gtagttgata aagttgctga ttgatttcgc
    47341 tgaatgccag tgcgcggtca acgcccagtt tctctggcgc ggcatcctgg cctaagcgac
    47401 gaccaaacca gatctctgcc gtcaggtcgc gaacgcggtt aaacaaaacg ctgtggttat
    47461 gagtgtcatc gcttttaatc agcaccagca ccgcttccgg ttcgttaaag ccggtgaagt
    47521 accagaagtc actgttctga cgataggggt attcgctgtc ggcgctacgt gttacttctg
    47581 gtgcagcaaa aatcagcgcg gcgctgccgg gttgcatttg ctctaccagg gcctgacggc
    47641 gacgctgaaa ctcttgccgg gatatatcac tcataacact ctccttacat tttttgtttt
    47701 tagtgtagag tcggtttttg tacttctggc gcggtcggtt gcggatgagt aaaggtgtcg
    47761 tggcataaca gcgcggcaac acgaacgtac tcgatgatct cttcaagcga catttcaagc
    47821 tcttcctgat cttcgtcttc gtcgtaaccc aactgcgcga tgttacgcag atcgtcgatg
    47881 gcttcaccgg tttcgccggt cactttgtcc agcttcggtt gcgtaacgcc aagaccaagc
    47941 aggaagtgat tgacccaacc agccagcgca tcagcccgat cgaaaacgct gacatcatcg
    48001 ccatcaggca gataaagctg aaaaaggaag ccgtcatcct gcagggcatc gctggtggca
    48061 gagtgcattt tgcgcagtgc ctgtgccagc tcatgaccga aagccatgcc ttcgttcgtc
    48121 aggtcgtgaa gtagcggcag ccatgagctg tcatcgttac cgccacatat aatcccactg
    48181 attaaaccat gcatctcagc aggggtcaga cccgtccctt gttggttcag atactggttc
    48241 atttcgttgt aaccaggcat ttcgttctgt atagacataa gcattcgtcg tcaaagggag
    48301 gaatattcat gatatgctac cactttgggc cctggtggac cagaaaaggg cttgtctctt
    48361 ctcatcaggg tagctatagt gtcgcccctt cgcagaccat gggtctaaag acgaaggcag
    48421 cgcagtcaat cagcaggaag gtggcatgtc tgcacaaccc gtcgatatcc aaatttttgg
    48481 ccgttcactg cgtgtgaact gcccgcctga ccaaagggat gcgttgaatc aggcagcgga
    48541 cgatctgaac caacggttgc aagatctgaa agaacgcact agagtcacaa atactgaaca
    48601 gttggtcttc attgccgcat tgaatatcag ctatgagtta gcgcaagaaa aagcgaagac
    48661 tcgtgactac gcggcaagta tggaacagcg tattcggatg ctgcagcaga ccatagaaca
    48721 agcgttactt gaacaaggtc gcatcaccga aaaaactaac caaaactttg aatgacactt
    48781 ttcggtttac tgtggtagag taaccgtgaa gacaaaattt ctctgagatg ttcgcaagcg
    48841 ggccagtccc ctgagccgat atttcatacc acaagaatgt ggcgctccgc ggttggtgag
    48901 catgctcggt ccgtccgaga agccttaaaa ctgcgacgac acattcacct tgaaccaagg
    48961 gttcaagggt tacagcctgc ggcggcatct cggagattcc cttcttatct ggcaccagcc
    49021 atgacgcaac taccagaact cccactgaca ttatcccgac aagaaatccg caaaatgatt
    49081 cggcaacgtc gtcgtgcgtt aacgccggaa caacagcagg aaatgggtca acaagccgct
    49141 acccggatga tgacttatcc cccggtggtg atggcacata cggtcgctgt attcctctct
    49201 tttgatggcg aactcgacac tcagccactc atagaacaac tctggcgcgc cggtaagcgc
    49261 gtatatcttc cagttttgca tccctttagt gccggtaatt tgctgttcct gaattaccat
    49321 ccgcaaagcg aactggtgat gaacaggttg aagatccatg agccaaaact ggatgtgcgt
    49381 gacgtgctac acctttcccg attagacgtg ttgatcacac cgctggtcgc ctttgatgag
    49441 tacggtcagc gcctgggaat gggcggtggt ttttatgatc ggaccttaca aaactggcag
    49501 cactataaaa tgcaaccggt gggttatgcg catgattgtc agttggtgga aaaactcccc
    49561 gttgaagagt gggatatccc tcttcctgcg gtggttacac cgtcgaaagt ctgggaatgg
    49621 taagggcgat acatcccgca tcgccctgat tgacatcgtt gattctttga cctaatttag
    49681 tgagtaaggg taagggagga tgactcctcc cctgagactg actgttaata agctgggaaa
    49741 cttatgagta acagtacaat cagtatgatg acaagtcgca tcataaccct tctccttcaa
    49801 gccctcgctt cggtgagggc tttaccgtta cagccccatg ctgccctgcc atcgtaaatc
    49861 cccattaaat aaacacaacg cattgatctg actttgattt attttctgga gcagactcgc
    49921 aatgtagaat gcgcaatgcg gcaacggtgt ggagaaggga taaaaaaaac gggcaagtca
    49981 gtgacctgcc cgttgatttt cagagaaggg gatttagtac agcagacgag cgcgaatggt
    50041 acccggaata gctttcattg cctgcagcgc tttttcggca acgtcttcgt cggcttcaat
    50101 atcaataact acataaccca tctgggcgga agtttgcaga tattgcgcgg cgatgttgac
    50161 gccctgctcg gcaaaaattt tgttgagcgc agtcagcaca cccggacggt tttcgtggat
    50221 gtgcatcaga cgacggccac cgtgcagtgg cagcgagact tccgggaagt tcaccgcaga
    50281 gagcgttgaa ccattatcag aatacttgat caatttaccc gcaacttcca gaccgatatt
    50341 ctcctgcgct tcctgagtcg aaccaccaat gtgtggcgtc agaaggacat tgtcgaattc
    50401 acacagcgga gaggtaaatg gatcgctatt ggtcgccggt tccgtcggga atacgtcgat
    50461 tgccgcccct gccagatgtt tgctcgccag cgcgtcacac agcgctggaa tatccaccac
    50521 agtaccgcgc gaagcattaa tcagcagcga gccgggtttc attagcgaaa tctctttcgc
    50581 gcccatcata tttttggtgg acggattctc tggtacatgc agactcacca catcgctcat
    50641 attcagcagg tcagaaagat gctgtacctg agtggcgttg cccagcggca gtttgttttc
    50701 aatatcataa aagtaaacat acattcccag cgattcagcc agaatgccca attgcgtacc
    50761 aatatgaccg tagccgatga tacccagctt tttgccgcgc gcttcaaaag aacccgccgc
    50821 cagtttgttc cacacgccac gatgcgcttt agcattggct tccggcacgc cgcgcaatag
    50881 cagcagcagt tcgccaatca ccagctccgc aacagagcgc gtatttgaga acggtgcgtt
    50941 aaataccggg atcccgcgct ttgccgccgc atccagatca acctggtttg ttccgataca
    51001 gaaacagcca atagcgacca gtttttctgc ggcgttgatc acgtcttcag tcagatgggt
    51061 acgggatcgc aggccgatga agtgggcatc gcggatggat tcttttaatt gttcatcatc
    51121 cagcgcgcct ttgtgaaatt cgatgttggt gtaaccagct gcacgaaggc tttccagcgc
    51181 cttttggtgc acgccttcta ccagcagaaa cttaatcttg tctttctcca gcgatacctt
    51241 tgccatttac ccaatcctgt cttttgaaat gttgtgtgcg gatttgcatc cgcctttcaa
    51301 catatcaaaa aataatattg cggcaatatg aacgtttgcg tcgcgatgtt gaggaaatat
    51361 cacgcaaaag tatttcttgg atgaatatgc tggtttagaa gatttattta gagcaatcga
    51421 caattgccag gtaaaaacgt gacacatgtc accaaattta atgaagagaa tttttttaac
    51481 gggggaggtt cccccgtcag atcatttcac aatagttttg acaccgtcag gtgtgccaat
    51541 cagcgcaacg tccgcgccac ggttagcaaa caaaccaaca gtcaccacgc caggaatcgc
    51601 atttatggcg ttttccatcg ctatcgggtc aaggatttcc atgccgtgga cgtcgaggat
    51661 cacgttgccg ttatcggtca ctacgccctg acggtattcc ggacgaccgc ccagtttcac
    51721 cagctggcgc gccactgcac tacgtgccat cgggataact tctactggca gcgggaattt
    51781 gcccagaata tcaacctgct tggaagcgtc tgcaatacag ataaattttt ccgcaaccga
    51841 agcaatgatt ttttcacggg tcagcgccgc gccgccgcct ttgatcattt gcatgtggcc
    51901 gttgatttca tctgcgccat caacgtagat gccaaggctg tcgacttcgt tgagatcaaa
    51961 aacgtgaatg ccgaggcttt tcagtttttc agtggaagca tctgaactgg aaacggcccc
    52021 ttcaatctgg cctttcattg taccgagtgc gtcaataaag tgtgcggcgg tggaacctgt
    52081 acctacacca acaatggtgc cgggctgaac atactgaagt gccgcccatc ctactgcttt
    52141 tttcaattca tcctgcgtca tgatcgtttc gcctgtggta tgaaatttca cacgcattat
    52201 atacaaaaaa agcgattcag accccgttgg caagccgcgt ggttaactca tccataaaat
    52261 atcgcgcaat ggcgggcatc ccctttcgcc ccgcaaataa agcatacaac ggtctgggta
    52321 tgccgctcca cggtgcaaac aggcgcacca gttccccgtt cgcaagcccc tgtttacagg
    52381 caaactgagg caataacgcc acgccattca acacaaccag ggcgcgaact cgctgagcga
    52441 aatgcattag cgcaaatccc tgatctttta agctgcgcgt taaccactga tacaaatgtt
    52501 acccgccaac ggcttcggta tatgcaaccc gacacaaaat tgtgtcatag tgcaggaaaa
    52561 agcatttacc aggagcagac aacagcaatg aaacgcccgg actacagaac attacaggca
    52621 ctggatgcgg tgatacgtga acgaggattt gagcgcgcgg cacaaaagct gtgcattaca
    52681 caatcagccg tctcacagcg cattaagcag ctggaaaata tgttcgggca gccgttgtta
    52741 gtgcgtaccg taccgccgcg cccgacggaa caagggcaaa aactgttggc gctactacgc
    52801 caggtggagc tgctggaaga agagtggctg ggcgatgaac aaaccggttc gactccgctg
    52861 ctgctttcac tggcggtcaa cgccgacagt ctggcgacgt ggttgcttcc ggcactggct
    52921 cctgtgttgg ctgattcgcc catccgcctc aacttgcagg tagaagatga aacccgcact
    52981 caggaacgtc tgcgtcgcgg cgaagtggtc ggcgcagtga gtattcaaca tcaggcgctg
    53041 ccgagttgtc ttgtcgataa actcggtgcg ctcgactatc tgttcgtcag ctcaaaaccg
    53101 tttgccgaaa aatatttccc taacggcgta acgcgttcgg cattactgaa agcgccagtg
    53161 gtcgcgtttg accatcttga cgatatgcac caggcctttt tgcagcaaaa tttcgatctg
    53221 cctcctggca gcgtaccctg ccatatcgtt aattcttcag aagcgttcgt gcaacttgct
    53281 cgccagggca ccacctgctg tatgatcccg cacctgcaaa tcgagaaaga gctggccagc
    53341 ggtgaactga ttgacttaac gcctgggcta tttcaacgac ggatgctcta ctggcaccgc
    53401 tttgctcctg aaagccgcat gatgcgtaaa gtcactgatg cgttgctcga ttatggtcac
    53461 aaagttcttc gtcaggatta atccatcaaa taacgcctga tagcacatat caggcgttat
    53521 cctcacttct ttttgtattc cttgaatcac atcacaaaat agacaaatct caggcggcaa
    53581 aaaacgacgt ctgaatgcat ttttttgctg gcgacaaacc cacgtaaaaa gctcaccgta
    53641 agcgcaaata ccctcatttt gattgcgttt tacggagcaa ataatgtcta acgtgcagga
    53701 gtggcaacag cttgccaaca aggaattgag ccgtcgggag aaaactgtcg actcgctggt
    53761 tcagcaaacc gcggaaggga tcgccatcaa gccgctgtat accgaagccg atctcgataa
    53821 tctggaggtg acgggtaccc ttcctggttt gccaccctac gttcgtggcc cgcgtgccac
    53881 tatgtatacc gcccaaccgt ggaccatccg tcagtatgct ggtttctcga cagcaaaaga
    53941 gtccaacgct ttttatcgcc gtaacctggc cgcccaccga ggctacgact ccgataaccc
    54001 acgcgtggcg ggcgacgtcg gcaaagcggg cgtcgctatc gacaccgggg aagatatgaa
    54061 agtcctgttc gaccagatcc cgctggataa aatgtcggtt tcgatgacca tgaatggcgc
    54121 agtgctacca gtactggcat tttatatcgt cgccgcagaa gagcaaggtg ttacatccga
    54181 taaactgacc ggcaccattc agaacgatat cctcaaagag tacctctgcc gcaataccta
    54241 tatttacccg ccaaaaccgt caatgcgtat tatcgcagac atcatcgcct ggtgttccgg
    54301 caacatgccg cgatttaata ccatcagtat cagcagttac cacatgggtg aagcgggtgc
    54361 caactgcgtg cagcaggtag catttacgct cgccgatagg attgagtaca tcaaagcagc
    54421 aatctccgcc gggctgaaaa ttgatgactt cgtccatcgc ctgtcgtttt tcttcggcat
    54481 tggcatagat ctgtttatga acgccgccat gttgcgtgcg gcacgttatt tatggagcga
    54541 agcggtcagt ggatttggcg cacaggatcc gtataacaac gttatccgca ccaccattga
    54601 tcattgtgca catcctatgt gtcgggatta tctgcatcgc tatctggaaa atgcgtccgg
    54661 cggacatatt caccacgatc ttagccacgt cttcgattta caccgtaatt taattgcaac
    54721 cggctcgatg ctgggttaat gactcgtccc gtcacgatct tcagcaatat gctgaagcat
    54781 cgtggcggta taacgatgat ttctgattga ataaagatat tcctgcatat acatcggcct
    54841 gtgcggcgca tcaaaatatt tcagcgttgc cggattcacc agtcgccagg caaaatgcgg
    54901 gatcaccgtc agaaaatgtc cccgctccac ggcgctgatt ttggccataa aactataggg
    54961 acgataaata atggttggat tgatcccaca aggacgcata ttcgcatcaa tcattgcttc
    55021 gaagttagcc cggttttgaa agcgcatttg tagccagggc agctcctgaa gcagctcttg
    55081 ctcctgtcgc ccttcataac ggcgagaaac gtgaaagccc aatcgcagcg gtggtagttc
    55141 actgatagtc aggttttcca gttcctgaac tcgtgcagat acatgctgtg gggagattat
    55201 aaaatctaac tgacggtcaa aaagattatc gataacgcca ttttcgctga actcaactgg
    55261 ctgggcggtc acgccttcgt acttatctca gagactaatc aactgatcaa aaataatggt
    55321 cggataagta ttatctatgc ctatcacaat gtctcgtgaa caacgtcctg agttatggat
    55381 ttcattatca atcgcagaaa gtcgttgata aaccgggaac agtttttgat acagttcctg
    55441 tccggcttta ttgaggctga tattattatc tttgcgggta aatagcgtat agccaatttg
    55501 ttcttccagc gcggcaatgc ttttgccaaa tcgcgaagcc gtcatatgga ttttttctgc
    55561 cgcccgggca atattattgg tttgcgctaa taaaatgaaa ttgcgcatct ttttcgaaat
    55621 aaaaatgtcc atcccctctc tcccgcatcc ttgtccgctt aaccgtttca gtcagcctat
    55681 ccttgatgaa accgcgagca aagataggtg attacgtcat ggttttacag aaaaaggagg
    55741 caatatcggg taaaggcatt agcccgacga atacgtcggg ctacaagtat taatgtgcag
    55801 caggtgtttt agcgggtggt tgatccacag gttctaactg gaagactaca tcgacctgat
    55861 catcaaactg aatagcggcc tgctcgtaag tttcctgtgc ggacaccggt gcagcatcag
    55921 ctttcatcat ccgcaccatt gggctgggct gatagttgga aacatgataa cgcacgctat
    55981 ataccggccc gagtttgcga ttaaagccat tcgccagttc ctgcgcctga tgaatcgcgt
    56041 tatcaatcgc tgccttacgc gctttgtctt tataggcatc cggctgcgcc acgcccagcg
    56101 acacagaacg aatttcgtta agacccgcct ttagcgcgcc gtccagcaag gaattcagtt
    56161 tgtctaactg acggagcgtg acttccaccg ttctcactgc gcggtagcct ttcaggatac
    56221 ttttaccatc ctgataatca taatctggct gagtgcgtaa gttcgctgag ctgatatctt
    56281 ttttcgcgat ctgattgagc tcaaggaagg aaatgtattg tgcgacgcgc tcatctgcct
    56341 gtttcttggc agtagcggca tccttcgcgg ccacgttaac ttcaatcgca agagtggcaa
    56401 tgtctggcac cgcatccacg cttgccgtac cggaggtgac aatatgcggt ccatccggca
    56461 attcgttagc ctgcgctgcc atcccgctaa tacccattaa tgccgccagg gcgataactt
    56521 tgaacttcac gttagtctct ccgtgttaat tgacttgccc aaaattcagg acctctggag
    56581 gcaagcttag cgcctctgtt ttatttttcc atcagatagc gcctaactga acaaggcttg
    56641 tgcatgagca ataccgtctc tcgccagctg caaggcaata aaccacataa cacatcccac
    56701 taccagattg ataatgcgct gtgattttgc cgtgcgcagg cgcggtgcca gccaggctgc
    56761 gagaatagcc agaccaaaga accacaggaa agaggcgcta attgttccga gcgcaaacca
    56821 acgttttggt tccacatcaa gttgcccgcc aaggctgccc agcacaacaa aagtatccag
    56881 gtaaacatgc ggattcagcc aggtcactgc caacatggtg gcgataattt tccatctgcc
    56941 ttgtttcaga acttcggcac tggctaactc aatgttactg ctcattgctg ttttaaaagc
    57001 gccaaaacca taccacagca agaaggctac gccgccccag gtgaccagcg ccagcaacca
    57061 cggcgactgc atcaataacg cgctgccacc aaaaatcccc gcacaaatca ggaccaaatc
    57121 gctgatagca caaagtaagg caatcataat gtggtactga cgacgaatgc cctgattcat
    57181 cacaaaagca ttttgtggac cgagcggtag gatcatagcc gccccaagtg caagaccttg
    57241 aaagtaataa gaaaacacgt tggatattcc gaattaagta tctgatgcag agactatatc
    57301 gcgggattgt tattagcgga aattgataat attaattggc aatcagaaaa actaataaga
    57361 gagaggccag agagtattat ctggcctcag cgttgattac gcagctttgt cttctttcac
    57421 ccgcttaaag ttcacatcca tttgcgggta cgggaagctg ataccggcgg catcaaattc
    57481 acgtttaata cgctccagca catcccagta cacgttttgc agatcgccgc tgttgctcca
    57541 gacgcggacc acgaaattaa tcgacgatgc gccaagttcg ttcagacgca cagtcatttc
    57601 gcgatctttc aaaatgcgat cttcagactg gataatatcg gtcaggatct gcttaacctg
    57661 atcgatatcg gaatcatacg ccacgccaat aataaattcg ttacggcgag ctggctcgcg
    57721 ggagaagtta ataatattcc cggcaataat tttaccgttc ggaataacga taattttacc
    57781 gtctgcagta cgcatggtgg tggagaaaat ctgcacactc agcacagtac cggctacgcc
    57841 gccgaggtca acatattctc cggcacggaa cgggcggaac atgacaagta acacgccagc
    57901 ggccaggtta gacagtgacc cctgcaaagc cagaccaaca gctaagcctg cggcaccgag
    57961 tacagcaatg actgacgctg tttgtacgcc cacgcgcccc agtgcagcga ttagcgtaaa
    58021 ggcgataatt ccgtaacgga ctaatgcaga aagaaaatca gcaacagtgg catcgatttt
    58081 acgggagatc atcaggcgat tcaccgcgtt ggaaatcatc cgcgcgataa tcaaaccaac
    58141 gatgatgatc gcgagtgccg ccacgatgtt tactgcataa cttagcagca gcgcctggtt
    58201 agccaccagc cagcttcccg cgccgtttat gctatcgaca acattcaaat cttccattca
    58261 atattccttt tcaactgact caaaatggag aaatacactc cgccgcctta tgacgggcag
    58321 tctgacaagg gtaaacaata aagcataatt ttgccaaata gatcacagat ttctcatctg
    58381 tatcaccttg cttttggcga aaaaaagacc cgcagagcgg gccttgagat aagcagaaag
    58441 gaatgactta cagaacgtcg atcgcgttca gttcctggaa tgctttttcc agacgagcga
    58501 tcatcgaagt ctgaccggca cgcagccata cgcgcggatc gtagtatttc ttgttcggct
    58561 gatcttcgcc tttcgggtta cccagctgac cctgcaggta agcttcgttc gctttgtagt
    58621 agttcagaac gccttcccag gttgcccatt gggtatcggt atcgatgttc attttcacta
    58681 cgccgtagct tacggagtct ttgatttcct gagcagtaga accggaacca ccgtggaata
    58741 cgaagttcag gctgttgtgc ggcaggttgt gtttcttaga aacatattcc tgagagtcgc
    58801 gcaggatggt cggagtcaga accacgttac ccggcttgta aacaccgtgt acgttaccga
    58861 aggaggcagc gatggtgaaa cgcgggctga ttttgctcag ctcggtatac gcataatcaa
    58921 cgtcttccgg ctgggtgtac agtgcagaag cgtccatgtg gctgttgtcc acgccgtctt
    58981 cttcaccacc ggtgcaaccc agttcgattt ccagagtcat gccgattttg gacatgcgct
    59041 ccaggtattt agagcagatt tcgatgttct cttgcagaga ttcttcagac aggtcgatca
    59101 tgtgagaaga gaacagcggc ttgccggtag ctgcgaagtg tttttcaccc gcgtccagca
    59161 gaccgtcgat ccacggcagc agtttcttcg cgcagtggtc ggtgtgcagg ataaccggaa
    59221 caccataatg ttcagccatc tggtgaacgt gatgcgcacc agagatcgcg cccaggatag
    59281 cagcaccctg cggaacgtca gatttcacgc ctttaccagc gataaaggaa gcaccaccgt
    59341 tggagaactg aacgataacc ggcgctttaa ctttagcagc ggtttccagc acggcgttga
    59401 tggagtcagt accgacgcag tttactgctg gcagtgcgaa gttgttttct tttgctacct
    59461 ggaaaacttt ctgtacgtca tcaccagtga ttacgccagg ttttacgaaa tcaaaaatct
    59521 tagacatgtc tcttgtcctg tatcgtcggg ccttatactc gtcatacttc aagttgcatg
    59581 tgctgcgtct gcgttcgctc accccagtca cttacttatg taagctcatg ggattcactc
    59641 tcttgtcgcc ttcctgcaac tcgaattatt tagagtatga aaaagtgcgt gctttaaaag
    59701 cgcgctgcaa caagggcagg tttccctgcc ctgtgatttt ttacttctta gcgcgctctt
    59761 cgagcatcgc tactgcaggc agtactttac cttccacgaa ttcgaggaat gcgccgccgc
    59821 cagtggagat gtaggagatt ttgtcagcaa tgccgaacag gtcgattgct gccagagtgt
    59881 cgccgccgcc agcgatggag aacgcttcgc tgtctgcgat agcgttagcc acgatttcag
    59941 tacctttgcg gaagttcggg aattcgaaca cgccaaccgg accgttccac agaatggttt
    60001 tcgcattctt caggatttca gccagttcct gagcggaagc atcaccgata tccaggatct
    60061 gctcgtcagc tttcacatcg ttaacagatt tcagggtagc cggtgcagtt tcagagaact
    60121 cggttgctac gcgaacatca gacggaaccg ggatgttgca ggtggtcagc agacgtttag
    60181 cttcgtcaac caggtcagct tcgtacaggg atttacccac atcgtggcct tgtgccgcga
    60241 taaaggtgtt agcgatacca ccgccaacga tcagctggtc agcgatttta gacagggagt
    60301 ccagaacggt cagtttggta gatactttag aaccaccaac gatagccacc atcgggcgag
    60361 caggttcttt cagtgcttta cccagcgcgt ccagttcagc tgccagcagc gggcctgcgc
    60421 acgcaacgtc agcgaattta ccgataccgt gagtagaagc ctgcgcgcgg tgagcagtac
    60481 cgaatgcgtc cattacgaac acgtcacaca gtgcagcgta ttttttggac agagtttcgt
    60541 catctttctt ctcgcctttg ttgaagcgaa cgttttccag aacaaccagt tcaccttcag
    60601 caacgtcaac gccgtcgagg taatctttaa ccagacgaac cgggttagac agtttgtctt
    60661 tcaggtagtt aacaaccggc agcagagaga attcttcgtt gtactcgcct tcggtaggac
    60721 gacccaggtg ggaagttacc atcacttttg cgccttgttt cagggccagt tcgatggtcg
    60781 gcagagaagc acggatacgc gcgtcgctgg ttactttccc gtcttttact ggtacgttca
    60841 gatccgcacg gataaataca cgtttcccag caagatccag atcggtcatc ttaattacag
    60901 acatggtgaa tcctctcgtt gattctaaaa gttttgcaga cgctgcttgc gtcttacctg
    60961 aaagcaacag tagccatagc taacgtcgtg tcgagcattc ggttagcaaa gccccattcg
    61021 ttatcgcacc agaccaacgt tttgatcagg tgtgcgccac tgacccgggt ttgagtgcca
    61081 tcgacaatgg cactgtgcgg atcgtggtta aaatctacag agaccaacgg caattccgta
    61141 tagtcaacta taccatgaaa tgaaccttgt gctgcttttt gcagcaacag gttgacttca
    61201 ttggctttta caggtttctt caccgtcacg cttaaatcga ttgccgtcac atttatggtt
    61261 ggcacacgta ccgcaatcgc ttcaaagcga tcgttaaatt gcggaaaaaa tcgtgtgata
    61321 ccggcggcca gtttagtatc gaccggaatg atcgactggc tggctgcccg ggtgcgacgc
    61381 aggtcaggat ggtatgcatc aataacctgt tgatcgtgca tggcggagtg aattgtggtc
    61441 acagtgccgg actcaatacc gtacgcatca tctaacaatt tgatgacggg aattatgcaa
    61501 ttcgtggtac aggaagcgtt agaaacgatg cggtgttccg cacgaagttg atcctgattg
    61561 acgccgtaaa caacggtcgc gtcgagatcg ttactgccag gatgtgaaaa gagcactttt
    61621 ttggccccgg cggcaatatg cgcttcgcca tgctcacggg agccatatac gccggtgcag
    61681 tcgaggacta catcaacgcc aagttcacgc caggggagcg attgcagtga acgttcatgc
    61741 aatacgcgga tggcgtcatc accaacaaaa agttgatcgc gttcctgtcg tacttcccat
    61801 gcaaaacggc catggctggt gtcatatttc aacaaatgcg ccatgcccgc agcatccgcc
    61861 agttcgttga ttgccaccac ggtaatttcc gcccggcgtc cggattcata caaagcacga
    61921 accacattac gcccgatgcg accgaagcca tttatcgcta cgcgtacggt catagatctc
    61981 ctgcaaggtt ttccctgagc aaatttgcca gacagagtaa tccagcaaat cgttcgggga
    62041 aaccttacct gtcgcaaact gcgactgatt ggttaattgt cgaacattta atcgactgaa
    62101 acgcttcagc taggataagc gaaacgtgga ataaaaggaa tgattgtcca gtcaaagaag
    62161 acaattatct gacttacgtc acgttttcag ccccgcccct gtggaattta ttccactttg
    62221 cagaatcacg aataagcgta gcaaaatctt ttcgttgtgc cgtcacaaac cctgacttat
    62281 atcagaatat gactgatgca gcgtcgatat tatccgcaat acgtccgagt ttcggagtac
    62341 tccaatgatg acattgccct acccatattc ttcctccgca cgtttttacg cgctgcgcct
    62401 gctccccggg caggaagttc tctcccaatt acgcgccttc gcgcagcaac aacaactcca
    62461 cgccgcgtgg attgcaggtt gtaccggcag tctgactgac gtcgccttac gctatgccgg
    62521 acaagaaaac actgccctgc taagcggtaa gttcgaagtc atcgcattga acggcacgct
    62581 ggaacagagt ggtgaacatc ttcatctctg cgtttccgat ccacacggca cgatgcttgg
    62641 cggtcatatg atgcccggct gtaccgtgcg gaccacgctt gaactggtga tcggctgcct
    62701 tgaggaactg gcgtttagcc gccagtcttg cgcgctttct ggttacgacg agttgcatat
    62761 ttccccccgt taaataacta ctttcagtga ggttttctta tggcgcgtcg ccacttttcc
    62821 agtcaggcat tggtgttgat cgttatctct atcgccatca atatgatcgg cgggcaactg
    62881 gccagcatgg tcaaattacc tatttttctc gactccatcg gcacacttat tagcgcggta
    62941 ctgcttggtc cggtgatcgg gatgctaacc ggtttactga ccaatttact ctggggattg
    63001 ctgaccgacc cgatcgccgc cgcatttgcg cccgtcgcga tggtcatcgg cctggtcgcc
    63061 ggttggctgg cgcgagcggg ttggtttcgc accctgccaa aagtcgttgt tagcggcgtg
    63121 attattacgc ttgcggtaac ggtcgtcgct gtaccgctgc gtactgcgct gttcggcggc
    63181 gtgacaggaa gcggagccga tttgttcgtt gcctggatgc actcgatggg gcaaaacctg
    63241 gtggagtcgg tggcgattac ggtgataggc gcgaacctgg tcgataaaat tcttacagcg
    63301 gtgattgtct ggcttttact gcgtcagctg ccgattcgca ccacgcgcca ttttccggca
    63361 atggctgccg tgcgctaaat gcatccgttt acatccttaa cgctgtgggc gctggcggcc
    63421 tgcaccacgc ttatcctgcc cacacagaca atattgccag tctatagcgc ggcgaccttt
    63481 ttctgcctta ttgcgctaaa ggccacgcgg cggcgggcaa aatatgtcgt ctggctgatg
    63541 ttctcgctgg gggctggact gtggctggtg cacggtggct ggctgacaga atggctaagc
    63601 ggcacgccac gctctccaga acggtgggcg catgctatta cgctctggtt gcggattctg
    63661 gcgattgttt caacctcaca gttgtggatg cagtacgtcc ccgtgcaacg ttttatccgc
    63721 gctttgtttg cttctcgcct gccgccgggc gtcgcttatc tgtttgccgg gccgttattg
    63781 gtcgttgagc agttaaaacg gcaattggcg attattcatg aagcgcagcg cgcccgcggc
    63841 gtgccgctgg atgagggctg gtatcagcgt ttacgtgcga tgcccgcgtt aattattccg
    63901 ctaacacaca atgcgctaaa cgatcttgct gtccgtggtg cggcgctgga tatgcgggca
    63961 tttcgaatca acaataggcg cactacgtta tgggcaccag ccgatagcac cctacaacgc
    64021 gtagcacgtt acgccatgat attgctaatg ctggctgaat tcggagcgtg gatatggtta
    64081 cgttagaaca gtttcgctat tgcccgacac actcaacgca tcctccgttc tgttatgact
    64141 ttcactatgt caaaccgggg atggtcgcca tctttggtga caacggcagt ggcaaaagta
    64201 cgctggcgca gctgatggcg ggctggtatc cggactttct accaggagag ataaccggca
    64261 cggggacgct tctcggtacg cccattgggc gtctcccgct taatgaacaa tccgccacca
    64321 tccagctcgt ccagcaatcg ccctatttgc agctctccgg ctgtaccttt agcgtggaag
    64381 aagaagttgc ctttgggcca gaaaatctgt gtcttgctga aaaagagatt atggcgcgga
    64441 ttgacgccgc cctggccctg accgagtgcc agccgttgcg ccatcgtcac ccggcaacac
    64501 tctccggtgg agagacacag cgtgtggtca tcgcctgcgc catcgcgatg cagccaaagc
    64561 tcttaattct tgatgaagcc tttagccgcc tgacgccgca ggcccgcgaa atgctgttgc
    64621 agcgcttaca acactgggcg cttgaacgtg gttcgctgat tattcttttt gaacgtcatc
    64681 acacaccttt cctgaaccac tgccagcaag cgtggcaatt gcaaaacgga gcgctccagc
    64741 cactatgtta acgttaaatc agatatctta tcgctggcct ggcgcggcaa cggattgtct
    64801 ttgcgacatt tctctgcaac tcaaacaagg tgaatggctg gcgctgaccg gcgacaatgg
    64861 cgcgggaaaa tcgacgctgt tacgagtaat ggcaggtctt cttacgccca cagccggtac
    64921 ggttatgctg caacaacaag cgatgaaaaa cctgaaaaac cgccagcgcg cggcaaagat
    64981 tggcgtactg tttcaggaag cggaaaatca gctttttcac agcaccgttg ctgacgaaat
    65041 cgcatttggt ttgaaactgc aaaaatgccc ggcggatgaa atcactcaac gtaccaatgc
    65101 tgccctgcaa tgctgtcagt tagcagacac tgccagcgcg catccgttag atctgcatag
    65161 tgcgcagcga cggatggtcg ccgtcgcctg cctggaagca ctctctccgc cactattgct
    65221 actggacgag ccgagtcggg attttgacga aaattggttg agcgtgtttg aaagctggct
    65281 ggagaagtgc cgccagcgag gcacgagcgt cgtagcaatc agtcatgacg ccgcatttac
    65341 ccggcgtcat ttctctcgtg tggtgcgact ggaagatggt gtaatcagga acgttaatcc
    65401 atcagacgat attcacccgt agcggtcatc attaaggtca aatttgcagg cagactctct
    65461 tccagcaccc ggcgaacatt cggcccgtcg gtacggtcat aaaaggcttc tgcgtctgcc
    65521 agcgataacc ctcccgccag cttgcgcccc accagacgct cccgcaacgc agatgcggga
    65581 gctttgataa agatcgagaa atcacaaaat tgcgcaagct ggcaccactt ttcatcatcc
    65641 agcaacaacc agtttccttc gacgatgacg agtggtgcgg taacgtgcag cgcattttca
    65701 acaggatcat gcttttgtcg atcgtactgc ggccacgtac aatccccttc cactacctgg
    65761 cgcagatttt ccgccagttt cgcaacgttg aatgtctctg gtgcgccttt gaaggggcgc
    65821 aattgatgca catccagcca gctattgtaa tggtgaaaac catccatcgg cagcgtctga
    65881 atagcgggca gttccgggtc ttgttgcgcg agatattccc agaaggtggt tagggtagat
    65941 ttacccgtcc ccggcggagc gcaaagaaaa accaccgtac gccgctgcgg atttactgtc
    66001 tgtaaggccg ccagcatacg cagcaacggt ttatgtacat tttcaatttc atcatcagag
    66061 tactgcgcct gtacctttag tccattcacc gtcagttcga ttttcacggc gttaattcct
    66121 ttaaggtggt cgttactgcc agcgtcagcg ccgttttgac catcccgcta aaacgcgcct
    66181 ctgaccagac ggcgaaatcg gcgaatttca tcgctttcaa cgcgtcatag agactcgcat
    66241 tgcggattat gctattgacg ttgctgataa aatcccaggc catgtcatcg gcaaatccgg
    66301 gcgtttcatc ctgttcgttc aggtaatgcc aaaactggga aaaatgcgtg atatcggcgt
    66361 acagccattt atccattttc cccagcgcat aaatcaaacg cagcgcaaca tcaatattgt
    66421 caagcgggcc gctttgggcg agtaacggct tcactgcata ttcgacgatc tcttcatcgt
    66481 tatcgacaaa cagcgacggc agaaactggc gtatcccctg gagcaaaata gaatgcgcag
    66541 ttttcataaa tgaaaataaa ttgtcctgtg catccagttg ctcaagcaca tcatcttctg
    66601 tcagcgtcgc cattggtctt ccgatagatc accatcaatg cagggagtct attatattac
    66661 acgtttggtc cgtgccgccg atcagtaatg tctgcgtccg caccccattc gccgtctgtt
    66721 ggataccgtt caccaactcg ccgtccgtca cgcccgtagc gctaaagagg atatcgttcc
    66781 ccctcgccga taaccactcg cccacgaaaa gcaacgtcgt ttaatgcctg gcgcatcgcg
    66841 gtgaccgcaa ggccatcaat tttatttttg tcgccacagc cggtttgcgg ccaggcagcc
    66901 aacgctgcct gttccgtaac gcgaaataat ggccacgcca gagacatcat tgcgcggcct
    66961 ccccaatatc aacgccaaat tgtgccagca gatatttctc ggcctgttca ttccagatgc
    67021 cgtgcgtttc ctcaaccagc cgcgccagct ctttaaacag cggatcggtt ttgccttttt
    67081 cggcaaaatc ggcaatggcg gtcagcggca tggtcacgcc gttataaatg agttttttac
    67141 cgccagggat atccggcaga ttgagcacgg tttctggcac cgcatccagg ccaccgatat
    67201 gggtcaccat aaacgacggc tgtaactgcc ccgtggcgct aagggcaatc gcctctttca
    67261 tgtcgtccgt ggaaccgccg gacgtgccga caatgtgcgt actgttgtaa tggacgttgt
    67321 agaaattaaa cggcactttg aagtttttat ccgtcggccc ggcaaagaag ttcaggcagc
    67381 catcttccgc cagtaattca tcagccatct cgacgactga aggcaccgct gcatagacaa
    67441 atacgtcatc aaatccggca tctcctgtca gcgcccgcag cgtctggacc ggatcgctca
    67501 cccctttggt attcatatac accagctcaa tgcctttact ggccgccagt tccaccggca
    67561 gcagcttctg cacctgcgcc agccgtttgt cgtcgatatc gactaccacc acccgcgacg
    67621 gttgtatgcc gccgttaata gcgtaatcga tagcgccaat tcccatcggg cctgcacacg
    67681 ccagtagcgc aatattgccg ccaggtttga cgcccatgcg atgctcataa acatattgcg
    67741 tggtgtgata attggcatga taagcgccaa taatgcagca cataggctct gccagcgacg
    67801 ccgcagcaaa ataagagccg tgatagggta atacgcagcc caaattaatg gcgatttcgg
    67861 gaataatcat ataggtggcg ttaccgccaa aatattcgta gctgtagccc gctgaatacc
    67921 cgcctggtaa ccccatcgcc ggttgcaata caaaacgctg gcctttttta tatttgccag
    67981 tgagattttt acccacttcg acaataaccc cagcacattc atgcccggta atgaccggat
    68041 gattttctaa atcgtcgggg acgcgtttat gttcactacc gagtaacgcc gctttccagg
    68101 tggataaaca aacgctgtca gaaattacat tcactaacag ttcattatcg gtaatttctg
    68161 gcagctcaaa ttcgcgcaga cggacatccc gcttgccata aatagcagca actttggttt
    68221 tcatttttac ctctgtaatc agtgtttatg ttcagcggtt aattgattaa ataaagtgtc
    68281 gagttttgga tcgccaatat agttattaat cagtatcagc ggtttatccg tcacacgttt
    68341 cacgcgccct tccagactgg catgagtaac aacgatatcc gcatccgcag gcacgttttc
    68401 tatcgcgtaa tgttttactt caattgccag ccccgctttt tccagacgtt tacggaaggt
    68461 ggttgcgccc atcgcgctgg agcccatccc cgcatcgcaa acaaaggcga tacgcttaac
    68521 ccggcttaac gagaatgccc cctcttgttt catcgcctta accgcattgg cggactgagc
    68581 aaactcatct tcgctcttcg tttccaccgt tttttccatc ttcagtatca gcgaagtgat
    68641 agcaaaggac accagggtac ctaccgtaac accggcaatt gtcgccagga acgagccttt
    68701 cggcgtcagt gccaggtaag caaagataga ccccggactt gggccagcca ccagaccacc
    68761 gtccagtaag ttaaacatcc aggtgccaga cataccgccc gcaatcatgg caataatggt
    68821 cagcggcttc atcagcacat acgggaaata cagttcgtgg atcccaccga ggaagtgaat
    68881 aatcatcgcc ccgggcgcag aacgtttact catcccttta ccaaacaagg taaacgccag
    68941 cagcagcccc agaccaggac ctggattgga tgccaccata aagaagatgg atttaccgtt
    69001 aaccgaagcc tgttgcattc ccagcggata atagacgccc tgatcgatcg cattattaag
    69061 gaaaagcact ttcgccggtt cattgatgac tgacagcaaa ggtagataac ccgcatgtac
    69121 cagcgcctca atacactctt tgacgaaagt attagcaatt aacaccgccg ggccgataac
    69181 ttcaaaaccc agcagacaaa ggagcatccc cgcgatacct aatgagaagt tattgataac
    69241 catctcaaaa ccagcaggta tgcgtttttc cagcgacttg tcgatatatt taatcaccag
    69301 accaccgagc ggccccataa tcatcgagcc aaggaacatc gggatttcag cgccgacgat
    69361 cacaccaatc gtaccaattc cgcccatgac cgcgccgcgt ttaccgccga ccagatgacc
    69421 accggtagaa ccaatcatca cgggcaataa ataggtaatc atcggaccaa caattttggc
    69481 gaaatgttca ttcggcagcc agccggtggg aataaataat gccgttataa atccccaggc
    69541 aataaaagca ccgatattgg ggatgaccat tgcagtcaaa aatcccccaa aagcctggac
    69601 ctttgcacga gcagacttgt tttccataat attatcctgt agaggagaga gtaattatcc
    69661 gcggctgata atgtccgcta attgtttttc tgatgatgcg gttaagagtt gttcgagtat
    69721 ttcttcttcg cacagtaatt cacttaacgc ctgaatagca ccaatgtgcg aatcggcatc
    69781 ggcagcagaa agtccgatga gtaatttaat cggttcgtca ttccccggaa aataaacgcc
    69841 ttgtttaagt aacgtcaaag acatcccggt tttaagcgct ccacattccg gtcgcgcatg
    69901 aggcattgcc acgcctggtg cgagaatata ataaggacca ttgctgatgg tggaaccttt
    69961 aattgcctga atgtaattct cgctgatata gtttttatcc agcaatgata ccatcgagaa
    70021 atcgatagct tcctgccaat cttttgctga atgtataact gagattgatg actctggaaa
    70081 ataatcaata agccgcataa attatcctta ttttattttt agggtacagt ccggcggtaa
    70141 aaataaaat
//
LOCUS       DADSYM010000002        64982 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2415
            SAMN08039579-rid11723173.denovo.002, whole genome shotgun sequence.
ACCESSION   DADSYM010000002 DADSYM010000000
VERSION     DADSYM010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039579
            Sequence Read Archive: SRR6362533
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 64982)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 64982)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980162.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 03:39:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,484
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 4,347
            CDSs (with protein)               :: 4,347
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 43 of 85
            Pseudo Genes (incomplete)         :: 37 of 85
            Pseudo Genes (internal stop)      :: 19 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..64982
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039579-rid11723173.denovo.002"
                     /strain="LGE2415"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8302 N 95.9628 W"
                     /collection_date="2015-08-24"
     gene            complement(<1..456)
                     /gene="folK"
                     /locus_tag="J1Y83_000069"
     CDS             complement(<1..456)
                     /gene="folK"
                     /locus_tag="J1Y83_000069"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HBA7628263.1"
                     /translation="MTVAYIAIGSNLASPLEQVNAALKALGDIPESRILAVSSFYRTP
                     PLGPQDQPDYLNAAVALKTTLAPEELLNHTQRIELQQGRVRKAERWGPRTLDLDIMLF
                     GNEVINTERLTVPHYDMKNRGFMLWPLFEIAPELVFPDGLSLVETLQAKG"
     gene            complement(453..1871)
                     /gene="pcnB"
                     /locus_tag="J1Y83_000070"
     CDS             complement(453..1871)
                     /gene="pcnB"
                     /locus_tag="J1Y83_000070"
                     /EC_number="2.7.7.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000174652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polynucleotide adenylyltransferase PcnB"
                     /protein_id="HBA7628264.1"
                     /translation="MTLPRCTIFTRVANFCRKVLSREESEAEQAVARPQVTVIPREQH
                     AISRKDISENALKVMYRLNKAGYEAWLVGGGVRDLLLGKKPKDFDVTTNATPEQVRKL
                     FRNCRLVGRRFRLAHVMFGPEIIEVATFRGHHEGNVSDRTTSQRGQNGMLLRDNIFGS
                     IEEDAQRRDFTINSLYYSVADFTVRDYVGGMKDLKDGVIRLIGNPETRYREDPVRMLR
                     AVRFAAKLGMRISPETAEPIPRLATLLNDIPPARLFEESLKLLQAGYGYETYKLLCEY
                     HLFQPLFPTITRYFTENGDSPMERIIEQVLKNTDTRIHNDMRVNPAFLFAAMFWYPLL
                     ETAQKIAQESGLTYHDAFALAMNDVLDEACRSLAIPKRLTTLTRDIWQLQLRMSRRQG
                     KRAWKLLEHPKFRAAYDLLALRAEVERNAELQRLVKWWGEFQVSAPPDQKGMLNELDE
                     EPSPRRRTRRPRKRAPRREGTA"
     gene            complement(1910..2836)
                     /gene="gluQRS"
                     /locus_tag="J1Y83_000071"
     CDS             complement(1910..2836)
                     /gene="gluQRS"
                     /locus_tag="J1Y83_000071"
                     /EC_number="6.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308175.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA glutamyl-Q(34) synthetase GluQRS"
                     /protein_id="HBA7628265.1"
                     /translation="MLPPYFLFKEMTDTHYIGRFAPSPSGELHFGSLIAALGSYLQAR
                     ARQGRWLVRIEDIDPPREVPGAAETILRQLEHYGLYWDGDVLWQSQRHHAYREALAWL
                     HEQGLSYYCTCTRARIQSIGGIYDGHCRDLHHGPDNAAVRIRQQHPVTQFTDLLRGII
                     HADEKLAREDFIIHRRDGLFAYNLAVVVDDHFQGVSEIVRGADLIEPTVRQISLYQLF
                     GWKVPDYIHLPLALNPQGAKLSKQNHAPALPKGDPRPVLIAALHFLGQQVETHWQDFS
                     VEQILQSAVKNWTLTAVPESAIVNSTFSNASC"
     gene            complement(2873..3328)
                     /gene="dksA"
                     /locus_tag="J1Y83_000072"
     CDS             complement(2873..3328)
                     /gene="dksA"
                     /locus_tag="J1Y83_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-binding protein DksA"
                     /protein_id="HBA7628266.1"
                     /translation="MQEGQNRKTSSLSILAIAGVEPYQEKPGEEYMNEAQLAHFRRIL
                     EAWRNQLRDEVDRTVTHMQDEAANFPDPVDRAAQEEEFSLELRNRDRERKLIKKIDKT
                     LKKVEDEDFGYCESCGVEIGIRRLEARPTADLCIDCKTLAEIREKQMAG"
     gene            complement(3506..4210)
                     /gene="sfsA"
                     /locus_tag="J1Y83_000073"
     CDS             complement(3506..4210)
                     /gene="sfsA"
                     /locus_tag="J1Y83_000073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HBA7628267.1"
                     /translation="MEFSPPLQRATLIQRYKRFLADVITPDGRELTLHCPNTGAMTGC
                     ATPGDTVWYSTSDNTKRKYPHTWELTQSQSGAIICVNTLWANRLTKEAILNESISELA
                     GYSSLKSEVKYGAERSRIDFMLQADSRPDCYIEVKSVTLAENEQGYFPDAVTERGQKH
                     LRELMSVAAEGQRAVIFFAVLHSAITRFSPARHIDEKYAQLLSEAQQRGVEILAYKAE
                     LSAKGMALKKSLPVTL"
     gene            complement(4225..4755)
                     /gene="thpR"
                     /locus_tag="J1Y83_000074"
     CDS             complement(4225..4755)
                     /gene="thpR"
                     /locus_tag="J1Y83_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414689.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HBA7628268.1"
                     /translation="MSEPQRLFFAIDLPAEIREQIIRWRATHFPPEAGRPVAADNLHL
                     TLAFLGEVSAEKEKALSLLAGRIRQPGFTLTLDDAGQWLRSRVVWLGMRQPPRGLIQL
                     ANMLRSQAARSGCFQSNRPFHPHITLLRDASEAVTIPPPGFNWSYTVTEFTLYASSFA
                     RGRTRYTPLKRWALTQ"
     gene            4829..7258
                     /gene="hrpB"
                     /locus_tag="J1Y83_000075"
     CDS             4829..7258
                     /gene="hrpB"
                     /locus_tag="J1Y83_000075"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase HrpB"
                     /protein_id="HBA7628269.1"
                     /translation="MSSLPVAAVLPELLAALDGASQVLLSAPTGAGKSTWLPLQLLAH
                     PGINGKIILLEPRRLAARNVAQRLAELLNEKPGDTVGYRMRAQNCVGPNTRLEVVTEG
                     VLTRMIQRDPELSGVGLVILDEFHERSLQADLALALLLDVQQGLRDDLKLLIMSATLD
                     NDRLQQMLPGAPVVISEGRSFPVERRYLPLPAHQRFDEAVAVATAEMLRQESGSLLLF
                     LPGVGEIQRVQEQLASRIGSDVLLCPLYGALSLNDQRKAILPAPQGMRKVVLATNIAE
                     TSLTIEGIRLVVDCAQERGAHFDPRTGLTRLITQRVSQASMTQRAGRAGRLEPGICLH
                     LIAKEQAERAAAQSEPEILQSDLSGLLMELLQWGCSDPAQMSWLDQPPTVNLLAAKRL
                     LRMLGALDGERLSAQGQKMATLGNDPRLAAMLVSAKNDDEAATAAKIAAILEEPPRMG
                     NSDLGVAFSRNQPAWQQRSQQLLKRLNVRGGEADSSLIAPLLARAYADRIARRRGQDE
                     RYQLANGMGAMLDADDALSRHEWLIAPLLLQGSASPDARILLALPVDIDELVQRCPQL
                     VQQSDTVEWDDAQGTLKAWRRLQIGQLMVKVQPLAKPSEDELHQAMLNGIRDKGLSVL
                     NWTAEAEQLRLRLLCAAKWLPEYDWPAVDDESLLATLETWLLPHMTGVHSLRGLKSLD
                     IYQALRGLLDWVMQQRLDSELPAHYTVPTGSRIAIRYHEDNPPALAVRMQEMFGEATN
                     PTIAQGRVPLVLELLSPAQRPLQITRDLSAFWKGAYREVQKEMKGRYPKHVWPDDPAN
                     TAPTRRTKKYS"
     gene            7352..9880
                     /gene="mrcB"
                     /locus_tag="J1Y83_000076"
     CDS             7352..9880
                     /gene="mrcB"
                     /locus_tag="J1Y83_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glycosyl
                     transferase/transpeptidase"
                     /protein_id="HBA7628270.1"
                     /translation="MAGNDREPIGRKGKPTRPVKQKVSRRRYEDDDDYDDYDDYEDEE
                     PMPRKGKGKGRKPRGKRGWLWLLLKLAIIFAVMIAIYGVYLDQKIRSRIDGKVWQLPA
                     AVYGRMVNLEPDMTISKNEMVKLLEATQYRQVSKMTRPGEFTVQANSIEMIRRPFDFP
                     DSKEGQVRARLTFDGDHLATIVNMENNRQFGFFRLDPRLITMISSPNGEQRLFVPRSG
                     FPDLLVDTLLATEDRHFYEHDGISLYSIGRAVLANLTAGRTVQGASTLTQQLVKNLFL
                     SSERSYWRKANEAYMALIMDARYSKDRILELYMNEVYLGQSGDNEIRGFPLASLYYFG
                     RPVEELSLDQQALLVGMVKGASIYNPWRNPKLALERRNLVLRLLQQQQIIDQELYDML
                     SARPLGVQPRGGVISPQPAFMQLVRQELQAKLGDKVKDLSGVKIFTTFDSVAQDAAEK
                     AAVEGIPALKKQRKLSDLETAIVVVDRFSGEVRAMVGGSEPQFAGYNRAMQARRSIGS
                     LAKPATYLTALSQPKIYRLNTWIADAPIALRQPNGQVWSPQNDDRRYSESGRVMLVDA
                     LTRSMNVPTVNLGMALGLPAVTETWIKLGVPKDQLNPVPAMLLGALNLTPIEVAQAFQ
                     TIASGGNRAPLSALRSVIAEDGKVLYQSFPQAERAVPAQAAYLTLWTMQQVVQRGTGR
                     QLGAKYPNLHLAGKTGTTNNNVDTWFAGIDGSTVTITWVGRDNNQPTKLYGASGAMSI
                     YQRYLANQTPTPLNLVPPEDIADMGVDYDGNFVCSGGMRVLPVWTSDPQSLCQQSEMQ
                     QQPSGNPFDQSSQPQQQPQQQPAQQEQKDSDGVAGWIKDMFGSN"
     gene            10100..12358
                     /gene="fhuA"
                     /locus_tag="J1Y83_000077"
     CDS             10100..12358
                     /gene="fhuA"
                     /locus_tag="J1Y83_000077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrichrome porin FhuA"
                     /protein_id="HBA7628271.1"
                     /translation="MAPSKTAQPKHSLRKIAVVVATAVSGMSVYAQAAVEPKEDTITV
                     TAAPAPQESAWGPAATIAARQSATGTKTDTPIQKVPQSISVVTAEEMALHQPKSVKEA
                     LSYTPGVAVGTRGASNTYDYLIIRGFAADGQSQNNYLNGLKLQGNFYNDAVIDPYMLE
                     RAEIMRGPVSVLYGKSSPGGLLNMVSKRPTTEPLKEVQFKAGTDSLFQTGFDFSDALD
                     DDGVYSYRLTGLARSANAQQKGSEEQRYAIAPAFTWRPDDKTNFTFLSYFQNEPETGY
                     YGWLPKEGTVEPLPNGKRLPTDFNEGAKNNTYSRNEKMIGYSFDHEFNDTFTVRQNLR
                     FAQNKVSQKSVYGYGMCSDPLYTKDQEALKASPCLSIPQSQWGHTLTRQYVIDNEKLE
                     NFSVDTQLQSKFATGSVDHTLLTGVDFMRMRNDIDSWFGYAGSVAPSDIYNLDRSDFD
                     FGAHPNPSGPYRVLLKQKQTGLYVQDQAQWDKVLVTLGGRYDWADQSSFNRDYGNKSE
                     RDDKEFTWRGGVNYLFDNGVTPYFSYSESFEPASQTDANGDLFAPSKGKQYEVGVKYV
                     PEDRPIVVTGALYQLTKTNNLMADPNGSLFSVEGGEIRARGVELEAKAALSASVNVVG
                     SYTYTDAEYTTDTTYKGNTPAQVPKHMASLWADYTFFDGPLSGLTLGTGGRYTGSSYG
                     DPANSFKVGSYTVVDALVRYDLARVGMAGSNVALHVNNLFDREYVASCFNTYGCFWGA
                     ERQVVATATFRF"
     gene            12409..13206
                     /gene="fhuC"
                     /locus_tag="J1Y83_000078"
     CDS             12409..13206
                     /gene="fhuC"
                     /locus_tag="J1Y83_000078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe3+-hydroxamate ABC transporter ATP-binding
                     protein FhuC"
                     /protein_id="HBA7628272.1"
                     /translation="MQEYTNHSDTTFALRNISFRVPGRTLLHPLSLTFPAGKVTGLIG
                     HNGSGKSTLLKMLGRHQPPSEGEILLDAQPLESWSSKAFARKVAYLPQQLPPAEGMTV
                     RELVAIGRYPWHGALGRFGAADREKVEEAISLVGLKPLAHRLVDSLSGGERQRAWIAM
                     LVAQDSRCLLLDEPTSALDIAHQVDVLALVHRLSQERGLTVIAVLHDINMAARYCDYL
                     VALRGGEMIAQGTPAEIMRGETLEMIYGIPMGILPHPAGAAPVSFVY"
     gene            13206..14096
                     /gene="fhuD"
                     /locus_tag="J1Y83_000079"
     CDS             13206..14096
                     /gene="fhuD"
                     /locus_tag="J1Y83_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-hydroxamate ABC transporter
                     substrate-binding protein FhuD"
                     /protein_id="HBA7628273.1"
                     /translation="MSGLPLISRRRLLTAMALSPFLWQMNTAHAAVIDPNRIVALEWL
                     PVELLLALGIVPYGVADTINYRLWVSEPPLPDSVIDVGLRTEPNLELLTEMKPSFMVW
                     SAGYGPSPEMLARIAPGRGFNFSDGKQPLAMARKSLTEMADLLNLQSAAETHLTHYED
                     FIRSMKPRFVKRGARPLLLTTLIDPRHMLVFGPNSLFQEILDEYGIPNAWQGETNFWG
                     STAVSIDRLAAYKDVDVLCFDHDNSKDMDALMATPLWQAMPFVRAGRFQRVPAVWFYG
                     ATLSAMHFVRILDNAIGGKA"
     gene            14093..16075
                     /gene="fhuB"
                     /locus_tag="J1Y83_000080"
     CDS             14093..16075
                     /gene="fhuB"
                     /locus_tag="J1Y83_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-hydroxamate ABC transporter permease
                     FhuB"
                     /protein_id="HBA7628274.1"
                     /translation="MSKRIALFPVLLLALLVVAAAALTWMNFSQALPRSQWAQAAWSP
                     DIDVIEQMIFHYSLLPRLAISLLVGAGLGLVGVLFQQVLRNPLAEPTTLGVATGAQLG
                     ITVTTLWAIPGAMASQFAALAGACVVGLIVFGVAWGKRLSPVTLILAGLVVSLYCGAI
                     NQLLVIFHHDQLQSMFLWSTGTLTQTDWGGVERLWPQLLGGVMLTLLLLRPLTLMGLD
                     DGVARNLGLALSLARLAALSLAIVISALLVNAVGIIGFTGLFAPLLAKMLGTRRLLPR
                     LMLASLIGALILWLSDQIILWLTRVWMEVSTGSVTALIGAPLLLWLLPRLRSISAPDM
                     KVNDRVATERQHVLAFALAGGVLLLMAVVVALSFGRDAHGWTWASGALLEDLMPWRWP
                     RIMAALFAGVMLAVAGCIIQRLTGNPMASPEVLGISSGAAFGVVLMLFLVPGNAFGWL
                     LPAGSLGAAVTLLIIMIAAGRGGFSPHRMLLAGMALSTAFTMLLMMLQASGDPRMAQV
                     LTWISGSTYNATDAQVWRTGIVMVILLAITPLCRRWLTILPLGGDTARAVGMALTPTR
                     IALLLLAACLTATATMTIGPLSFVGLMAPHIARMMGFRRTMPHIVISALVGGLLLVFA
                     DWCGRMVLFPFQIPAGLLSTFIGAPYFIYLLRKQSR"
     gene            complement(16110..17390)
                     /gene="hemL"
                     /locus_tag="J1Y83_000081"
     CDS             complement(16110..17390)
                     /gene="hemL"
                     /locus_tag="J1Y83_000081"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HBA7628275.1"
                     /translation="MSKSENLYSAARELIPGGVNSPVRAFTGVGGTPLFIEKADGAYL
                     YDVDGKAYIDYVGSWGPMVLGHNHPAIRNAVIEAAERGLSFGAPTEMEVKMAQLVTEL
                     VPTMDMVRMVNSGTEATMSAIRLARGFTGRDKIIKFEGCYHGHADCLLVKAGSGALTL
                     GQPNSPGVPADFAKHTLTCTYNDLASVRAAFEQYPQEIACIIVEPVAGNMNCVPPLPE
                     FLPGLRALCDEFGALLIIDEVMTGFRVALAGAQDYYGVEPDLTCLGKIIGGGMPVGAF
                     GGRRDVMDALAPTGPVYQAGTLSGNPIAMAAGFACLNEVAQPGVHETLDELTTRLAEG
                     LREAAEEAGIPLVVNHVGGMFGIFFTDAESVTCYQDVMACDVERFKRFFHMMLDEGVY
                     LAPSAFEAGFMSVAHSMEDINNTIDAARRVFAKL"
     gene            17615..19036
                     /gene="clcA"
                     /locus_tag="J1Y83_000082"
     CDS             17615..19036
                     /gene="clcA"
                     /locus_tag="J1Y83_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="H(+)/Cl(-) exchange transporter ClcA"
                     /protein_id="HBA7628276.1"
                     /translation="MKTDTPSLETPQAARLRRRQLIRQLLERDKTPLAILFMAAVVGT
                     LVGLAAVAFDKGVAWLQNQRMGALVHTADNYPLLLTVAFLCSAVLAMFGYFLVRKYAP
                     EAGGSGIPEIEGALEDQRPVRWWRVLPVKFFGGLGTLGGGMVLGREGPTVQIGGNIGR
                     MVLDVFRLKGDEARHTLLATGAAAGLAAAFNAPLAGILFIIEEMRPQFRYTLISIKAV
                     FIGVIMSTIMYRIFNHEVALIDVGKLSDAPLNTLWLYLILGIIFGIFGPIFNKWVLGM
                     QDLLHRVHGGNITKWVLMGGAIGGLCGLLGFVAPATSGGGFNLIPIATAGNFSMGMLV
                     FIFVARVITTLLCFSSGAPGGIFAPMLALGTVLGTAFGMVAVELFPQYHLEAGTFAIA
                     GMGALLAASIRAPLTGIILVLEMTDNYQLILPMIITGLGATLLAQFTGGKPLYSAILA
                     RTLAKQEAEQLARSKAASARENT"
     gene            19060..19125
                     /locus_tag="J1Y83_000083"
     CDS             19060..19125
                     /locus_tag="J1Y83_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA7628277.1"
                     /translation="MAIIIGLEFAQLPMSFGAKYE"
     gene            19118..19462
                     /gene="erpA"
                     /locus_tag="J1Y83_000084"
     CDS             19118..19462
                     /gene="erpA"
                     /locus_tag="J1Y83_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster insertion protein ErpA"
                     /protein_id="HBA7628278.1"
                     /translation="MSDDVALPLEFTDAAANKVKSLIADEDNPNLKLRVYITGGGCSG
                     FQYGFTFDDQVNEGDMTIEKQGVGLVVDPMSLQYLVGGSVDYTEGLEGSRFIVTNPNA
                     KSTCGCGSSFSI"
     gene            complement(19509..20132)
                     /locus_tag="J1Y83_000085"
     CDS             complement(19509..20132)
                     /locus_tag="J1Y83_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRIC cation channel family protein"
                     /protein_id="HBA7628279.1"
                     /translation="MLVYWLDIVGTAVFAISGVLLAGKLRMDPFGVLVLGVVTAVGGG
                     TIRDMALDHGPVFWVKDPTDLVVAMVTSMLTIVLVRQPRRLPKWMLPVLDAVGLAVFV
                     GIGVNKAFNAEAGPLIAVCMGVITGVGGGIIRDVLAREIPMILRTEIYATACIIGGIV
                     HATAYYTFSVPLETASMMGMVVTLLIRLAAIRWHLKLPTFALDENGR"
     gene            complement(20170..20970)
                     /gene="btuF"
                     /locus_tag="J1Y83_000086"
     CDS             complement(20170..20970)
                     /gene="btuF"
                     /locus_tag="J1Y83_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter substrate-binding
                     protein BtuF"
                     /protein_id="HBA7628280.1"
                     /translation="MAKSLFRALVALSFLAPLWLNAAPRVITLSPANTELAFAAGITP
                     VGVSSYSDYPPQAQKIEQVSTWQGMNLERIVALKPDLVIAWRGGNAERQVDQLASLGI
                     KVMWVDATSIEQIANALRQLAPWSPQPDKAEQAAQSLLDQYAQLKAQYADKPKKRVFL
                     QFGINPPFTSGKESIQNQVLEVCGGENIFKDSRVPWPQVSREQVLARSPQAIVITGGP
                     DQIPKIKQYWGEQLKIPVIPLTSDWFERASPRIILAAQQLCNALSQVD"
     gene            complement(20963..21661)
                     /gene="mtnN"
                     /locus_tag="J1Y83_000087"
     CDS             complement(20963..21661)
                     /gene="mtnN"
                     /locus_tag="J1Y83_000087"
                     /EC_number="3.2.2.16"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/S-adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HBA7628281.1"
                     /translation="MKIGIIGAMEEEVTLLRDKIENRQTISLGGCEIYTGQLNGTEVA
                     LLKSGIGKVAAALGATLLLEHCKPDVIINTGSAGGLAPTLKVGDIVVSDEARYHDADV
                     TAFGYEYGQLPGCPAGFKADDKLIAAAEACIAELNLNAVRGLIVSGDAFINGSVGLAK
                     IRHNFPQAIAVEMEATAIAHVCHNFNVPFVVVRAISDVADQQSHLSFDEFLAVAAKQS
                     SLMVESLVQKLAHG"
     gene            21745..23262
                     /gene="dgt"
                     /locus_tag="J1Y83_000088"
     CDS             21745..23262
                     /gene="dgt"
                     /locus_tag="J1Y83_000088"
                     /EC_number="3.1.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dGTPase"
                     /protein_id="HBA7628282.1"
                     /translation="MAQIDFRKKINWHRRYRSPQGVKTEHEILRIFESDRGRIINSPA
                     IRRLQQKTQVFPLERNAAVRTRLTHSMEVQQVGRYIAKEILSRLKELKLLEAYGLDEL
                     TGPFESIVEMSCLMHDIGNPPFGHFGEAAINDWFRQRLYPEDAESQPLTDDRCSVAAL
                     RLRDGEEPLNALRRKIRQDLCHFEGNAQGIRLVHTLMRMNLTWAQVGGILKYTRPAWW
                     RGETPETHHYLMKKPGYYLSEEAYIARLRKELNLALYSRFPLTWIMEAADDISYCVAD
                     LEDAVEKRIFTVEQLYHHLHEAWGQHEKGSLFSLVVENAWEKSRSNSLSRSTEDQFFM
                     YLRVNTLNKLVPYAARRFIDNLPAIFAGTFNHALLEDASECSDLLKLYKNVAVKHVFS
                     HPDVEQLELQGYRVISGLLEIYRPLLNLPLSDFTELVEKERVKRFPIETRLFHKLSTR
                     HRLAYVEAVSKLPSDSPEFPLWEYYYRCRLLQDYISGMTDLYAWDEYRRLMAVEQ"
     gene            23392..24816
                     /gene="degP"
                     /locus_tag="J1Y83_000089"
     CDS             23392..24816
                     /gene="degP"
                     /locus_tag="J1Y83_000089"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine endoprotease DegP"
                     /protein_id="HBA7628283.1"
                     /translation="MKKTTLALSALALSLGLALSPLSATAAETSSATTAQQMPSLAPM
                     LEKVMPSVVSINVEGSTTVNTPRMPRNFQQFFGDDSPFCQEGSPFQSSPFCQGGLGGN
                     GGGQQQKFMALGSGVIIDADKGYVVTNNHVVDNATVIKVQLSDGRKFDAKMVGKDPRS
                     DIALIQIQNPKNLTAIKMADSDALRVGDYTVAIGNPFGLGETVTSGIVSALGRSGLNA
                     ENYENFIQTDAAINRGNSGGALVNLNGELIGINTAILAPDGGNIGIGFAIPSNMVKNL
                     TSQMVEYGQVKRGELGIMGTELNSDLAKAMKVDAQRGAFVSQVLPNSSAAKAGIKAGD
                     VITSLNGKPISSFAALRAQVGTMPVGSKLTLGLLRDGKQVNVNLELQQSSQNQVDSST
                     IFNGIEGAEMSNKGKDQGVVVNNVKTGTPAAQIGLKKGDVIIGANQQAVKNIAELRKV
                     LDSKPSVLALNIQRGDSTIYLLMQ"
     gene            24971..26128
                     /locus_tag="J1Y83_000090"
     CDS             24971..26128
                     /locus_tag="J1Y83_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CdaR family transcriptional regulator"
                     /protein_id="HBA7628284.1"
                     /translation="MAGWHLDTKMAQDIVARTMRIIDTNINVMDARGRIIGSGDRERI
                     GELHEGALLVLSQGRVVDIDDAVARHLHGVRQGINLPLRLEGEIVGVIGLTGEPENLR
                     KYGELVCMTAEMMLEQSRLMHLLAQDSRLREELVMNLIQAEENTPALTEWAQRLGIDL
                     NQPRVVAIVEVDSGQLGVDSAMAELQQLQNALTTPERNNLVAIVSLTEMVVLKPALNS
                     FGRWDAEDHRKRVEQLITRMKEYGQLRFRVSLGNYFTGPGSIARSYRTAKTTMVVGKQ
                     RMPESRCYFYQDLMLPVLLDSLRGDWQANELARPLARLKAMDNNGLLRRTLAAWFRHN
                     VQPLATSKALFIHRNTLEYRLNRISELTGLDLGNFDDRLLLYVALQLDEER"
     gene            complement(26182..26568)
                     /locus_tag="J1Y83_000091"
     CDS             complement(26182..26568)
                     /locus_tag="J1Y83_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000272186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3461 family protein"
                     /protein_id="HBA7628285.1"
                     /translation="MYDNLKSLGITNPEEIDRYSLRQEANNDILKIYFQKDKGEFFAK
                     SVKFKYPRQRKTVVADGVGQGYKEVQEISPNLRYIIDELDQICQRDRSEVDLKRKILD
                     DLRHLESVVTNKISEIEADLEKLTRK"
     gene            complement(26880..27704)
                     /gene="dapD"
                     /locus_tag="J1Y83_000092"
     CDS             complement(26880..27704)
                     /gene="dapD"
                     /locus_tag="J1Y83_000092"
                     /EC_number="2.3.1.117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase"
                     /protein_id="HBA7628286.1"
                     /translation="MQQLQNIIETAFERRAEITPANADTVTREAVNQVIALLDSGALR
                     VAEKIDGQWVTHQWLKKAVLLSFRINDNQVIEGAESRYFDKVPMKFANYDEARFQKEG
                     FRVVPPAAVRQGAFIARNTVLMPSYVNIGAYVDEGTMVDTWATVGSCAQIGKNVHLSG
                     GVGIGGVLEPLQANPTIIEDNCFIGARSEVVEGVIVEEGSVISMGVYIGQSTRIYDRE
                     TGEIHYGRVPAGSVVVSGNLPSKDGKYSLYCAVIVKKVDAKTRGKVGINELLRTID"
     gene            complement(27735..30407)
                     /gene="glnD"
                     /locus_tag="J1Y83_000093"
     CDS             complement(27735..30407)
                     /gene="glnD"
                     /locus_tag="J1Y83_000093"
                     /EC_number="2.7.7.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     uridylyltransferase/uridylyl-removing protein GlnD"
                     /protein_id="HBA7628287.1"
                     /translation="MNTLPEQYANTALPTLHGQPQNPCAWPRDELTVGGIKAHIDTFQ
                     RWLGDAFDNGISAEQLIEARTEFIDQLLQRLWIEAGFSQIADLALVAVGGYGRGELHP
                     LSDIDLLILSRKKLPDDQAQKVGELLTLLWDVKLEVGHSVRTLEECMLEGLSDLTVAT
                     NLIESRLLIGDVALFLELQKHIFSEGFWPSDKFYAAKVEEQNQRHQRYHGTSYNLEPD
                     IKSSPGGLRDIHTLQWVARRHFGATSLDEMVGFGFLTSAERAELNECLHILWRIRFAL
                     HLVVSRYDNRLLFDRQLSVAQRLNYSGEGNEPVERMMKDYFRVTRRVSELNQMLLQLF
                     DEAILALPADEKPRPIDDEFQLRGTLIDLRDETLFMRQPEAILRMFYTMVRNSAITGI
                     YSTTLRQLRHARRHLQQPLCNIPEARKLFLSILRHPGAVRRGLLPMHRHSVLGAYMPQ
                     WSHIVGQMQFDLFHAYTVDEHTIRVMLKLESFASEETRQRHPLCVDVWPRLPSTELIF
                     IAALFHDIAKGRGGDHSILGAQDVVHFAELHGLNSRETQLVAWLVRQHLLMSVTAQRR
                     DIQDPEVIKQFAEEVQTENRLRYLVCLTVADICATNETLWNSWKQSLLRELYFATEKQ
                     LRRGMQNTPDMRERVRHHQLQALALLRMDNIDEEALHQIWSRCRANYFVRHSPNQLAW
                     HARHLLQHDLSKPLVLLSPQATRGGTEIFIWSPDRPYLFAAVCAELDRRNLSVHDAQI
                     FTTRDGMAMDTFIVLEPDGSPLSADRHEVIRFGLEQVLIQSSWQPPQPRRQPAKLRHF
                     TVETEVTFLPTHTDRKSFLELIALDQPGLLARVGKIFADLGISLHGARITTIGERVED
                     LFIIATADRRALNNELQQEVHQRLTEALNPNDKG"
     gene            complement(30469..31263)
                     /gene="map"
                     /locus_tag="J1Y83_000094"
     CDS             complement(30469..31263)
                     /gene="map"
                     /locus_tag="J1Y83_000094"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HBA7628288.1"
                     /translation="MAISIKTPEDIEKMRVAGRLAAEVLEMIEPYVKPGVSTGELDRI
                     CNDYIVNEQHAVSACLGYHGYPKSVCISINEVVCHGIPDDAKLLKDGDIVNIDVTVIK
                     DGFHGDTSKMFIVGKPTIMGERLCRITQESLYLALRMVKPGINLREIGAAIQKFVEAE
                     GFSVVREYCGHGIGRGFHEEPQVLHYDSRETNVVLKPGMTFTIEPMVNAGKKEIRTMK
                     DGWTVKTKDRSLSAQYEHTIVVTDNGCEILTLRKDDTIPAIISHDE"
     gene            31631..32356
                     /gene="rpsB"
                     /locus_tag="J1Y83_000095"
     CDS             31631..32356
                     /gene="rpsB"
                     /locus_tag="J1Y83_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HBA7628289.1"
                     /translation="MATVSMRDMLKAGVHFGHQTRYWNPKMKPFIFGARNKVHIINLE
                     KTVPMFNEALAELNKIASRKGKILFVGTKRAASEAVKDAALSCDQFFVNHRWLGGMLT
                     NWKTVRQSIKRLKDLETQSQDGTFDKLTKKEALMRTRELEKLENSLGGIKDMGGLPDA
                     LFVIDADHEHIAIKEANNLGIPVFAIVDTNSDPDGVDFVIPGNDDAIRAVTLYLGAVA
                     ATVREGRSQDLASQAEESFVEAE"
     gene            32491..33342
                     /gene="tsf"
                     /locus_tag="J1Y83_000096"
     CDS             32491..33342
                     /gene="tsf"
                     /locus_tag="J1Y83_000096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HBA7628290.1"
                     /translation="MAEITASLVKELRERTGAGMMDCKKALTEANGDIELAIENMRKS
                     GAIKAAKKAGNVAADGVIKTKIDGNYGIILEVNCQTDFVAKDAGFQAFADKVLDAAVA
                     GKITDVEVLKAQFEEERVALVAKIGENINIRRVAALEGDVLGSYQHGARIGVLVAAKG
                     ADEELVKHIAMHVAASKPEFIKPEDVSAEVVEKEYQVQLDIAMQSGKPKEIAEKMVEG
                     RMKKFTGEVSLTGQPFVMEPSKTVGQLLKEHNAEVTGFIRFEVGEGIEKVETDFAAEV
                     AAMSKQS"
     gene            33489..34214
                     /gene="pyrH"
                     /locus_tag="J1Y83_000097"
     CDS             33489..34214
                     /gene="pyrH"
                     /locus_tag="J1Y83_000097"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000224576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HBA7628291.1"
                     /translation="MATNAKPVYKRILLKLSGEALQGTEGFGIDASILDRMAQEIKEL
                     VELGIQVGVVIGGGNLFRGAGLAKAGMNRVVGDHMGMLATVMNGLAMRDALHRAYVNA
                     RLMSAIPLNGVCDSYSWAEAISLLRNNRVVILSAGTGNPFFTTDSAACLRGIEIEADV
                     VLKATKVDGVFTADPAKDPTATMYEQLTYSEVLEKELKVMDLAAFTLARDHKLPIRVF
                     NMNKPGALRRVVMGEKEGTLITE"
     gene            34364..34921
                     /gene="frr"
                     /locus_tag="J1Y83_000098"
     CDS             34364..34921
                     /gene="frr"
                     /locus_tag="J1Y83_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000622421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HBA7628292.1"
                     /translation="MISDIRKDAEVRMDKCVEAFKTQISKIRTGRASPSLLDGIVVEY
                     YGTPTPLRQLASVTVEDSRTLKINVFDRSMSPAVEKAIMASDLGLNPNSAGSDIRVPL
                     PPLTEERRKDLTKIVRGEAEQARVAVRNVRRDANDKVKALLKDKEISEDDDRRSQDDV
                     QKLTDAAIKKIEAALADKEAELMQF"
     gene            35013..36209
                     /gene="ispC"
                     /locus_tag="J1Y83_000099"
     CDS             35013..36209
                     /gene="ispC"
                     /locus_tag="J1Y83_000099"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HBA7628293.1"
                     /translation="MKQLTILGSTGSIGCSTLDVVRHNPEHFRVVALVAGKNVTRMVE
                     QCLEFSPRYAVMDDEASAKLLKTMLQQQGSRTEVLSGQQAACDMAALEDVDQVMAAIV
                     GAAGLLPTLAAIRAGKTILLANKESLVTCGRLFMDAVKQSKAQLLPVDSEHNAIFQSL
                     PQPIQHNLGYADLEQNGVVSILLTGSGGPFRETPLRDLATMTPDQACRHPNWSMGRKI
                     SVDSATMMNKGLEYIEARWLFNASASQMEVLIHPQSVIHSMVRYQDGSVLAQLGEPDM
                     RTPIAHTMAWPNRVNSGVKPLDFCKLSALTFAAPDYDRYPCLKLAMEAFEQGQAATTA
                     LNAANEITVAAFLAQQIRFTDIAALNLSVLEKMDMREPQCVDDVLSVDANAREVARKE
                     VMRLAS"
     gene            36398..37156
                     /gene="ispU"
                     /locus_tag="J1Y83_000100"
                     /gene_synonym="uppS"
     CDS             36398..37156
                     /gene="ispU"
                     /locus_tag="J1Y83_000100"
                     /gene_synonym="uppS"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308203.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2E,6E)-farnesyl-diphosphate-specific
                     ditrans,polycis-undecaprenyl-diphosphate synthase"
                     /protein_id="HBA7628294.1"
                     /translation="MLSATQPLSEKLPAHGCRHVAIIMDGNGRWAKKQGKIRAFGHKA
                     GAKSVRRAVSFAANNGIEALTLYAFSSENWNRPAQEVSALMELFVWALDSEVKSLHRH
                     NVRLRIIGDTSRFNSRLQERIRKSEALTAGNTGLTLNIAANYGGRWDIVQGVRQLAEK
                     VQQGNLQPDQIDEEMLNQHVCMHELAPVDLVIRTGGEHRISNFLLWQIAYAELYFTDV
                     LWPDFDEQDFEGALNAFANRERRFGGTEPGDETA"
     gene            37169..38026
                     /gene="cdsA"
                     /locus_tag="J1Y83_000101"
     CDS             37169..38026
                     /gene="cdsA"
                     /locus_tag="J1Y83_000101"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HBA7628295.1"
                     /translation="MLKYRLISAFVLIPVVIAALFLLPPVGFAIVTLVVCMLAAWEWG
                     QLSGFTTRSQRVWLAVLCGLLLALMLFLLPEYHRNIHQPLVEISLWASLGWWIVALLL
                     VLFYPGSAAIWRNSKTLRIIFGVLTIVPFFWGMLALRAWHYDENHYSGAIWLLYVMIL
                     VWGADSGAYMFGKLFGKHKLAPKVSPGKTWQGFIGGLVTAAVISWGYGMWVNLDVAPV
                     TLLICSIVAALASVLGDLTESMFKREAGIKDSGHLIPGHGGILDRIDSLTAAVPVFAC
                     LLLLVFRTL"
     gene            38038..39390
                     /gene="rseP"
                     /locus_tag="J1Y83_000102"
     CDS             38038..39390
                     /gene="rseP"
                     /locus_tag="J1Y83_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma E protease regulator RseP"
                     /protein_id="HBA7628296.1"
                     /translation="MLSFLWDLASFIVALGVLITVHEFGHFWVARRCGVRVERFSIGF
                     GKALWRRTDKLGTEYVIALIPLGGYVKMLDERAEPVVPELRHHAFNNKSVGQRAAIIA
                     AGPVANFIFAIFAYWLVFIIGVPGVRPVVGEIAANSIAAEAQIAPGTELKAVDGIETP
                     DWDAVRLQLVDKIGDESTTITIAPFGSDQRRDVKLDLRHWAFEPDKEDPVSSLGIRPR
                     GPQIEPVLENVQPNSAASKAGLQAGDRIVKVDGQPLTQWVTFVMLVRDNPGKSLALEI
                     ERQGSPLSLTLIPESKPGNGKAIGFVGIEPKVIPLPDEYKVVRQYGPFNAIVEATDKT
                     WQLMKLTVSMLGKLITGDVKLNNLSGPISIAKGAGMTAELGVVYYLPFLALISVNLGI
                     INLFPLPVLDGGHLLFLAIEKIKGGPVSERVQDFCYRIGSILLVLLMGLALFNDFSRL
                     "
     gene            39420..41852
                     /gene="bamA"
                     /locus_tag="J1Y83_000103"
     CDS             39420..41852
                     /gene="bamA"
                     /locus_tag="J1Y83_000103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HBA7628297.1"
                     /translation="MAMKKLLIASLLFSSATVYGAEGFVVKDIHFEGLQRVAVGAALL
                     SMPVRTGDTVNDEDISNTIRALFATGNFEDVRVLRDGDTLLVQVKERPTIASITFSGN
                     KSVKDDMLKQNLEASGVRVGESLDRTTIADIEKGLEDFYYSVGKYSASVKAVVTPLPR
                     NRVDLKLVFQEGVSAEIQQINIVGNHAFTTDELISHFQLRDEVPWWNVVGDRKYQKQK
                     LAGDLETLRSYYLDRGYARFNIDSTQVSLTPDKKGIYVTVNITEGDQYKLSGVEVSGN
                     LAGHSAEIEQLTKIEPGELYNGTKVTKMEDDIKKLLGRYGYAYPRVQSMPEINDADKT
                     VKLRVNVDAGNRFYVRKIRFEGNDTSKDAVLRREMRQMEGAWLGSDLVDQGKERLNRL
                     GFFETVDTDTQRVPGSPDQVDVVYKVKERNTGSFNFGIGYGTESGVSFQAGVQQDNWL
                     GTGYAVGINGTKNDYQTYAELSVTNPYFTVDGVSLGGRLFYNDFQADDADLSDYTNKS
                     YGTDVTLGFPINEYNSLRAGLGYVHNSLSNMQPQVAMWRYLYSMGEHPSTSDQDNSFK
                     TDDFTFNYGWTYNKLDRGYFPTDGSRVNLTGKVTIPGSDNEYYKVTLDTATYVPIDDD
                     HKWVVLGRTRWGYGDGLGGKEMPFYENFYAGGSSTVRGFQSNTIGPKAVYFPHQASNY
                     DPDYDYECATQDGAKDLCKSDDAVGGNAMAVASLEFITPTPFISDKYANSVRTSFFWD
                     MGTVWDTNWDSSQYSGYPDYSDPSNIRMSAGIALQWMSPLGPLVFSYAQPFKKYDGDK
                     AEQFQFNIGKTW"
     gene            41974..42459
                     /gene="skp"
                     /locus_tag="J1Y83_000104"
                     /gene_synonym="hlpA"
                     /gene_synonym="ompH"
     CDS             41974..42459
                     /gene="skp"
                     /locus_tag="J1Y83_000104"
                     /gene_synonym="hlpA"
                     /gene_synonym="ompH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone Skp"
                     /protein_id="HBA7628298.1"
                     /translation="MKKWLLAAGLGLALATSAQAADKIAIVNMGSLFQQVAQKTGVSN
                     TLENEFKGRASELQRMETDLQAKMKKLQSMKAGSDRTKLEKDVMAQRQTFAQKAQAFE
                     QDRARRSNEERGKLVTRIQTAVKSVANSQDIDLVVDANAVAYNSSDVKDITADVLKQV
                     K"
     gene            42463..43488
                     /gene="lpxD"
                     /locus_tag="J1Y83_000105"
     CDS             42463..43488
                     /gene="lpxD"
                     /locus_tag="J1Y83_000105"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HBA7628299.1"
                     /translation="MPSIRLADLAQQLDAELHGDGDIVITGVASMQSAQTGHITFMVN
                     PKYREHLGLCQASAVVMTQDDLPFAKSAALVVKNPYLTYARMAQILDTTPQPAQNIAP
                     SAVIDATAKLGNNVSIGANAVIESGVELGDNVIIGAGCFVGKNSKIGAGSRLWANVTI
                     YHEIQIGQNCLIQSGTVVGADGFGYANDRGNWVKIPQIGRVIIGDRVEIGACTTIDRG
                     ALDDTVIGNGVIIDNQCQIAHNVVIGDNTAVAGGVIMAGSLKIGRYCMIGGASVINGH
                     MEICDKVTVTGMGMVMRPITEPGVYSSGIPLQPNKVWRKTAALVMNIDDMSKRLKSLE
                     RKVNQQD"
     gene            43593..44048
                     /gene="fabZ"
                     /locus_tag="J1Y83_000106"
     CDS             43593..44048
                     /gene="fabZ"
                     /locus_tag="J1Y83_000106"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000210741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HBA7628300.1"
                     /translation="MTTNTHTLQIEEILELLPHRFPFLLVDRVLDFEEGRFLRAVKNV
                     SVNEPFFQGHFPGKPIFPGVLILEAMAQATGILAFKSVGKLEPGELYYFAGIDEARFK
                     RPVVPGDQMIMEVTFEKTRRGLTRFKGVALVDGKVVCEATMMCARSREA"
     gene            44052..44840
                     /gene="lpxA"
                     /locus_tag="J1Y83_000107"
     CDS             44052..44840
                     /gene="lpxA"
                     /locus_tag="J1Y83_000107"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HBA7628301.1"
                     /translation="MIDKSAFVHPTAIVEEGASIGANAHIGPFCIVGPHVEIGEGTVL
                     KSHVVVNGHTKIGRDNEIYQFASIGEVNQDLKYAGEPTRVEIGDRNRIRESVTIHRGT
                     VQGGGLTKVGSDNLLMINAHIAHDCTVGNRCILANNATLAGHVSVDDFAIIGGMTAVH
                     QFCIIGAHVMVGGCSGVAQDVPPYVIAQGNHATPFGVNIEGLKRRGFSREAITAIRNA
                     YKLIYRSGKTLDEVKPEIAELAETYPEVKAFTDFFARSTRGLIR"
     gene            44840..45988
                     /gene="lpxB"
                     /locus_tag="J1Y83_000108"
     CDS             44840..45988
                     /gene="lpxB"
                     /locus_tag="J1Y83_000108"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HBA7628302.1"
                     /translation="MTEQRPLTIALVAGETSGDILGAGLIRALKERVPNARFVGVAGP
                     RMQAEGCEAWYEMEELAVMGIVEVLGRLRRLLHIRADLTKRFGELKPDVFVGIDAPDF
                     NITLEGNLKKQGIKTIHYVSPSVWAWRQKRVFKIGRATDLVLAFLPFEKAFYDKYNVP
                     CRFIGHTMADAMPLDPDKNGARDVLGIPYDAHCLALLPGSRGAEVEMLSADFLKTAQL
                     LRQTYPDLEIVVPLVNAKRREQFERIKAAVAPDLSVHLLDGMGREAMVASDAALLASG
                     TAALECMLAKCPMVVGYRMKPFTFWLAKRLVKTDYVSLPNLLAGRELVKELLQEECEP
                     QKLAAALLPLLANGKTSHAMHDTFRELHQQIRCNADEQAAQAVLELAQ"
     gene            45985..46581
                     /gene="rnhB"
                     /locus_tag="J1Y83_000109"
     CDS             45985..46581
                     /gene="rnhB"
                     /locus_tag="J1Y83_000109"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HBA7628303.1"
                     /translation="MIEFVYPHTQLVAGVDEVGRGPLVGAVVTAAVILDPARPIAGLN
                     DSKKLSEKRRLALCEEIKEKALSWSLGRAEPHEIDELNILHATMLAMQRAVAGLHIAP
                     EYVLIDGNRCPKLQMPSMAVVKGDSRVPEISAASILAKVTRDAEMAALDIVFPQYGFA
                     QHKGYPTAFHLEKLAEHGATEHHRRSFGPVKRALGLAS"
     gene            46618..50100
                     /gene="dnaE"
                     /locus_tag="J1Y83_000110"
     CDS             46618..50100
                     /gene="dnaE"
                     /locus_tag="J1Y83_000110"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HBA7628304.1"
                     /translation="MSEPRFVHLRVHSDYSMIDGLAKTAPLVKKAAALGMPALAITDF
                     TNLCGLVKFYGAGHGAGIKPIVGADFNVQCDLLGDELTHLTVLAANNTGYQNLTLLIS
                     KAYQRGYGTAGPIIDRDWLIELNEGLILLSGGRMGDVGRSLLRGNSALVDECVAFYEE
                     HFPDRYFLELIRTGRPDEESYLHAAVELAEARGLPVVATNDVRFIDSSDFDAHEIRVA
                     IHDGFTLDDPKRPRNYSPQQYMRSEEEMCELFADIPEALANTVEIAKRCNVTVRLGEY
                     FLPQFPTGDMSTEDYLVKRAKEGLEERLAFLFPDEEERLKRRPEYDERLDTELQVINQ
                     MGFPGYFLIVMEFIQWSKDNGVPVGPGRGSGAGSLVAYALKITDLDPLEFDLLFERFL
                     NPERVSMPDFDVDFCMEKRDQVIEHVADMYGRDAVSQIITFGTMAAKAVIRDVGRVLG
                     HPYGFVDRISKLIPPDPGMTLAKAFEAEPQLPEIYEADEEVKALIDMARKLEGVTRNA
                     GKHAGGVVIAPTKITDFAPLYCDEEGKHPVTQFDKSDVEYAGLVKFDFLGLRTLTIIN
                     WALEMINKRRAKNGEPPLDIAAIPLDDKKSFDMLQRSETTAVFQLESRGMKDLIKRLQ
                     PDCFEDMIALVALFRPGPLQSGMVDNFIDRKHGREEISYPDVQWQHESLKPVLEPTYG
                     IILYQEQVMQIAQVLSGYTLGGADMLRRAMGKKKPEEMAKQRSVFAEGAEKNGINAEL
                     AMKIFDLVEKFAGYGFNKSHSAAYALVSYQTLWLKAHYPAEFMAAVMTADMDNTEKVV
                     GLVDECWRMGLKILPPDINSGLYHFHVNDDGEIVYGIGAIKGVGEGPIEAIIEARNKG
                     GYFRELFDLCARTDTKKLNRRVLEKLIMSGAFDRLGPHRAALMNSLGDALKAADQHAK
                     AEAIGQADMFGVLAEEPEQIEQSYASCQPWPEQVVLDGERETLGLYLTGHPINQYLKE
                     IERYVGGVRLKDMHPTERGKVITAAGLVVAARVMVTKRGNRIGICTLDDRSGRLEVML
                     FTDALDKYQQLLEKDRILIVSGQVSFDDFSGGLKMTAREVMDIDEAREKYARGLAISL
                     TDRQIDDQLLNRLRQSLEPHRSGTIPVHLYYQRADARARLRFGATWRVSPSDRLLNDL
                     RGLIGSEQVELEFD"
     gene            50113..51072
                     /gene="accA"
                     /locus_tag="J1Y83_000111"
     CDS             50113..51072
                     /gene="accA"
                     /locus_tag="J1Y83_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HBA7628305.1"
                     /translation="MSLNFLDFEQPIAELEAKIDSLTAVSRQDEKLDINIDEEVHRLR
                     EKSVELTRKIFADLGAWQIAQLARHPQRPYTLDYVRLAFDEFDELAGDRAYADDKAIV
                     GGIARLDGRPVMIIGHQKGRETKEKIRRNFGMPAPEGYRKALRLMQMAERFKMPIITF
                     IDTPGAYPGVGAEERGQSEAIARNLREMSRLGVPVVCTVIGEGGSGGALAIGVGDKVN
                     MLQYSTYSVISPEGCASILWKSADKAPLAAEAMGIIAPRLKELKLIDSIIPEPLGGAH
                     RNPEAMAASLKAQLLADLADLDVLSTEDLKNRRYQRLMSYGYA"
     gene            51170..53311
                     /gene="ldcC"
                     /locus_tag="J1Y83_000112"
     CDS             51170..53311
                     /gene="ldcC"
                     /locus_tag="J1Y83_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine decarboxylase LdcC"
                     /protein_id="HBA7628306.1"
                     /translation="MNIIAIMGPHGVFYKDEPIKELESALVAQGFQIIWPQNSVDLLK
                     FIEHNPRICGVIFDWDEYSLDLCSDINQLNEYLPLYAFINTHSTMDVSVQDMRMALWF
                     FEYALGQAEDIAIRMRQYTNEYLDNITPPFTKALFTYVKERKYTFCTPGHMGGTAYQK
                     SPVGCLFYDFFGGNTLKADVSISVTELGSLLDHTGPHLEAEEYIARTFGAEQSYIVTN
                     GTSTSNKIVGMYAAPSGSTLLIDRNCHKSLAHLLMMNDVVPVWLKPTRNALGILGGIP
                     RGEFTRDSIEEKVAATTQAQWPVHAVITNSTYDGLLYNTDWIKQTLDVPSIHFDSAWV
                     PYTHFHPIYQGKSGMSGERVAGKVIFETQSTHKMLAALSQASLIHIKGEYDEEAFNEA
                     FMMHTTTSPSYPIVASVETAAAMLRGNPGKRLINRSVERALHFRKEVQRLREESDGWF
                     FDIWQPPQVDEAECWPVAPGEQWHGFSDADANHMFLDPVKVTILTPGMDEQGNMSEEG
                     IPAALVAKFLDERGIVVEKTGPYNLLFLFSIGIDKTKAMGLLRGLTEFKRSYDLNLRI
                     KNMLPDLYAEDPDFYRNMRIQDLAQGIHKLIRKHDLPGLMLRAFDTLPEMIMTPHQAW
                     QRQIKGEVETIALEQLVGRVSANMILPYPPGVPLLMPGEMLTEESRTVLDFLLMLCSV
                     GQHYPGFETDIHGAKQDEDGVYRVRVLKMAG"
     gene            53368..53757
                     /locus_tag="J1Y83_000113"
     CDS             53368..53757
                     /locus_tag="J1Y83_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308216.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HBA7628307.1"
                     /translation="MLGLKQVHHIAIIATDYAMSKAFYCDILGFTLQSEVYREARDSW
                     KGDLALNGQYVIELFSFPFPPERPSRPEACGLRHLAFSVDDIDAAVAHLESHNVKCEA
                     IRVDPYTQKRFTFFNDPDGLPLELYEQ"
     gene            53822..55135
                     /gene="tilS"
                     /locus_tag="J1Y83_000114"
     CDS             53822..55135
                     /gene="tilS"
                     /locus_tag="J1Y83_000114"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000176585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HBA7628308.1"
                     /translation="MTLTLNRQLLSSRQILVAFSGGLDSTVLLHQLVQWRTENPGVTL
                     RAIHVHHGLSANADAWVKHCENICQQWQVPLVVERVQLAQEGLGIEAQARQARYQAFA
                     RTLLPGEVLVTAQHLDDQCETFLLALKRGSGPAGLSAMAEVSEFAGTQLIRPLLARTR
                     GELVQWALAHGLRWIEDESNQDDSYDRNFLRLRVVPLLQQRWPHFAEATARSAALCAE
                     QESLLDELLADDLAHCQTPQGTLQIAPMLAMSDARRAAIIRRWLAGQNAPMPSRDALV
                     RIWQEVALAREDASPCLRLGAFEIRRYQSQLWWIKSVTGQSETILPWQTWLQPLELPA
                     GLGSVQLTAGGDIRPPRADEAVSVRFKAPGLLHIVGRNGGRKLKKIWQELGVPPWLRD
                     TTPLLFYGETLIAAAGVFVTQEGVAVGENGVSFVWQKTLSQVKAG"
     gene            complement(55169..55429)
                     /gene="rof"
                     /locus_tag="J1Y83_000115"
     CDS             complement(55169..55429)
                     /gene="rof"
                     /locus_tag="J1Y83_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rho-binding antiterminator"
                     /protein_id="HBA7628309.1"
                     /translation="MSMNDTYQPINCDDYDNLELACQHHLMLALELKDGEKLQAKASD
                     LVSRKNVEYLVVEAAGATRELRLDKITSFSHPEIGTVVVSES"
     gene            complement(55416..55616)
                     /locus_tag="J1Y83_000116"
     CDS             complement(55416..55616)
                     /locus_tag="J1Y83_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeP family protein"
                     /protein_id="HBA7628310.1"
                     /translation="MEKYCELIRKRYAEIASGDLGYVPDALGCVLKVLNEMAADDALS
                     EAVREKAAYAAANLLVSDYVNE"
     gene            55782..56327
                     /locus_tag="J1Y83_000117"
     CDS             55782..56327
                     /locus_tag="J1Y83_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeQ family protein"
                     /protein_id="HBA7628311.1"
                     /translation="MALKATIYKATVNVADLDRNQFLDTSLTLARHPSETQERMMLRL
                     LAWLKYADERLQFTRGLCADDEPEAWLRNDHLGIDLWIELGLPDERRIKKACTQAAEV
                     ALFAYNSRAAQIWWQQNQSKCAQFANLSIWYLDDEQLAKVSAFADRTMTLQATIQDGV
                     IWLSDDKNNLEVNLTAWQQPS"
     gene            56324..56746
                     /gene="arfB"
                     /locus_tag="J1Y83_000118"
     CDS             56324..56746
                     /gene="arfB"
                     /locus_tag="J1Y83_000118"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HBA7628312.1"
                     /translation="MIVISRHVAIPDGELEITAIRAQGAGGQHVNKTSTAIHLRFDIR
                     ASSLPEYYKERLLAASHHLISSDGVIVIKAQEYRNQELNREAALARLVAVIKDLTTEQ
                     KARRPTRPTRASKERRLASKAQKSSVKAMRGKVRSGRE"
     gene            56760..57470
                     /gene="nlpE"
                     /locus_tag="J1Y83_000119"
                     /gene_synonym="cutF"
     CDS             56760..57470
                     /gene="nlpE"
                     /locus_tag="J1Y83_000119"
                     /gene_synonym="cutF"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001591565.1"
                     /note="NlpE, an outer membrane lipoprotein, interacts
                     directly with CpxA, the sensor histidine kinase of the Cpx
                     system for response to envelope stress; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response activation lipoprotein
                     NlpE"
                     /protein_id="HBA7628313.1"
                     /translation="MVKKAIVTAMAVISLFTLMGCNNRAEVDTLSPAQAAELKPMPQS
                     WRGVLPCADCEGIETSLFLEKDGTWVMNERYLGAREEPSSFASYGTWARTADKLVLTD
                     SKGEKSYYRAKGDALEMLDREGNPIESQFNYTLEPAQSSLPMTPMTLRGMYFYMADAA
                     TFTDCATGKRFMVANNAELERGYLAARGNSEKPVLLSVEGHFTLEANPDTGAPTKVLA
                     PDTAGKFYPNQDCSSLGL"
     gene            complement(57626..58450)
                     /locus_tag="J1Y83_000120"
     CDS             complement(57626..58450)
                     /locus_tag="J1Y83_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeF family permuted papain-like enzyme"
                     /protein_id="HBA7628314.1"
                     /translation="MDKPKAYCRLLLPCFLLLSACTVDISQPDSSATVVNAEAKTWAV
                     KFQHQSSFTEQSIKEITEPDLKPGDLLFSSSLGVTSFGIRVFSTSSVSHVAIYLGDNN
                     VAEATGAGVQIVSLKKAIKHSDKLFVLRVPDLTPQQATEITAFANKIKDSGYNYRGIV
                     EFIPFMVTRQMCSLNPFSEDFRQQCVSGLAKAQLSSVGEGDKKSWFCSEFVTDAFAKA
                     GHPLTLAQSGWISPADLMHMRTGDISAFKPETQLQYVGHMKPGIYIKAGRFVGLTQ"
     gene            complement(58503..60221)
                     /gene="proS"
                     /locus_tag="J1Y83_000121"
     CDS             complement(58503..60221)
                     /gene="proS"
                     /locus_tag="J1Y83_000121"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HBA7628315.1"
                     /translation="MRTSQYLLSTLKETPADAEVISHQLMLRAGMIRKLASGLYTWLP
                     TGVRVLKKVENIVREEMNNAGAIEVLMPVVQPSELWQESGRWEQYGPELLRIADRGDR
                     PFVLGPTHEEVITDLIRNELSSYKQLPLNFYQIQTKFRDEVRPRFGVMRSREFLMKDA
                     YSFHTSQESLQETYDAMYAAYSKIFSRMGLDFRAVQADTGSIGGSASHEFQVLAQSGE
                     DDVVFSDTSDYAANIELAEAIAPKEPRAAATQEMTLVDTPNAKTIAELVEQFNLPIEK
                     TVKTLLVKAVEGSSFPLVALLVRGDHELNEVKAEKLPQVASPLTFATEEEIRAVVKAG
                     PGSLGPVNMPIPVVIDRTVAAMSDFAAGANIDGKHYFGINWDRDVATPEIADIRNVVA
                     GDPSPDGQGTLLIKRGIEVGHIFQLGTKYSEALKASVQGEDGRNQILTMGCYGIGVTR
                     VVAAAIEQNYDERGIVWPDAIAPFQVAILPMNMHKSFRVQELAEKLYSELRAQGIEVL
                     LDDRKERPGVMFADMELIGIPHTIVLGDRNLDNDDIEYKYRRNGEKQLIKTGDIVDYL
                     VKQIKG"
     gene            complement(60332..61039)
                     /gene="tsaA"
                     /locus_tag="J1Y83_000122"
     CDS             complement(60332..61039)
                     /gene="tsaA"
                     /locus_tag="J1Y83_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase
                     TrmO"
                     /protein_id="HBA7628316.1"
                     /translation="MSSFQFEQIGVIRSPYKEKFAVPRQPGLVKSANGELHLIAPYNQ
                     ADAVRGLEAFSHLWILFVFHQTMEGGWRPTVRPPRLGGNARMGVFATRSTFRPNPIGM
                     SLVELKEVVCHKDSVILKLGSLDLVDGTPVVDIKPYLPFAESLPDASASYAQSAPAAE
                     MAVCFTAEVEKQLLTLKKRYPQLTLFIREVLAQDPRPAYRKGEETGKTYAVWLHDFNV
                     RWRVTDAGFEVFALEPR"
     gene            complement(61036..61440)
                     /gene="rcsF"
                     /locus_tag="J1Y83_000123"
     CDS             complement(61036..61440)
                     /gene="rcsF"
                     /locus_tag="J1Y83_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rcs stress response system protein RcsF"
                     /protein_id="HBA7628317.1"
                     /translation="MRALPICLVALMLSGCSMLSRSPVEPVQSTAPQPKAEPAKPKAP
                     RATPVRIYTNAEELVGKPFRDLGEVSGDSCQASNQDSPPSIPTARKRMQINASKMKAN
                     AVLLHSCEVTSGTPGCYRQAVCIGSALNITAK"
     gene            complement(61558..62373)
                     /gene="metQ"
                     /locus_tag="J1Y83_000124"
     CDS             complement(61558..62373)
                     /gene="metQ"
                     /locus_tag="J1Y83_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter substrate-binding
                     lipoprotein MetQ"
                     /protein_id="HBA7628318.1"
                     /translation="MAFKFKTFAAVGALIGSLALVGCGQDEKDPNHIKVGVIVGAEQQ
                     VAEVAQKVAKDKYGLDVELVTFNDYVLPNEALSKGDIDANAFQHKPYLDQQLKDRGYK
                     LVAVGNTFVYPIAGYSKKIKSLDELQDGSQVAVPNDPTNLGRSLLLLQKVGLIKLKDG
                     VGLLPTVLDVVENPKNLKIVELEAPQLPRSLDDAQIALAVINTTYASQIGLTPAKDGI
                     FVEDKESPYVNLIVTREDNKDAENVKKFVQAYQSDEVYEAANKVFNGGAVKGW"
     gene            complement(62413..63066)
                     /gene="metI"
                     /locus_tag="J1Y83_000125"
     CDS             complement(62413..63066)
                     /gene="metI"
                     /locus_tag="J1Y83_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter permease MetI"
                     /protein_id="HBA7628319.1"
                     /translation="MSEPMMWLLVRGVWETLAMTFVSGFFGFVIGLPVGVLLYVTRPG
                     QIIANAKLYRTVSAIVNIFRSIPFIILLVWMIPFTRVIVGTSIGLQAAIVPLTVGAAP
                     FIARMVENALLEIPTGLIEASRAMGATPMQIVRKVLLPEALPGLVNAATITLITLVGY
                     SAMGGAVGAGGLGQIGYQYGYIGYNATVMNTVLVLLVILVYLIQFAGDRIVRAVTRK"
     gene            complement(63059..64090)
                     /gene="metN"
                     /locus_tag="J1Y83_000126"
     CDS             complement(63059..64090)
                     /gene="metN"
                     /locus_tag="J1Y83_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014168731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein
                     MetN"
                     /protein_id="HBA7628320.1"
                     /translation="MIKLSNITKVFHQGTRTIQALNNVSLHVPAGQIYGVIGASGAGK
                     STLIRCVNLLERPTEGSVLVDGQELTTLSESELTKARRQIGMIFQHFNLLSSRTVFGN
                     VALPLELDNTPKDEIKRRVTELLSLVGLGDKHDSYPSNLSGGQKQRVAIARALASNPK
                     VLLCDEATSALDPATTRSILELLKDINRRLGLTILLITHEMDVVKRICDCVAVISNGE
                     LIEQDTVSEVFSHPKTPLAQKFIQSTLHLDIPEDYQERLQAEPFTDCVPMLRLEFTGQ
                     SVDAPLLSETARRFNVNNNIISAQMDYAGGVKFGIMLTEMHGTQQDTQAAIAWLQEHH
                     VKVEVLGYV"
     gene            64278..64850
                     /gene="gmhB"
                     /locus_tag="J1Y83_000127"
     CDS             64278..64850
                     /gene="gmhB"
                     /locus_tag="J1Y83_000127"
                     /EC_number="3.1.3.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1,7-bisphosphate
                     7-phosphatase"
                     /protein_id="HBA7628321.1"
                     /translation="MAKSVPAIFLDRDGTINVDHGYVHEIDNFEFIDGVIDAMRELKK
                     MGFALVVVTNQSGIARGKFTEAQFETLTEWMDWSLADRDVDLDGIYYCPHHPQGSVEE
                     FRQVCDCRKPHPGMFLSARDYLHIDMAASYMVGDKLEDMQAAAAASVGTKVLVRTGKP
                     ITPEAENAADWVLNSLADLPQAIKKQQKPA"
BASE COUNT        15300 a        16142 c        17901 g        15639 t
ORIGIN      
        1 gccttttgcc tgtagagttt caactaatga taagccatca ggaaatacca attccggcgc
       61 aatttcaaac agtggccaca gcataaagcc acgatttttc atatcgtagt gcggaacggt
      121 cagacgttcg gtatttatta cttcattacc aaacaacatg atgtcgagat ccagcgtgcg
      181 cggtccccag cgttcagctt tacggacgcg accttgctgc aattcgatac gctgtgtgtg
      241 attgagtagc tcttcagggg caagagtggt tttcagcgcg acggcggcat ttaagtaatc
      301 gggttgatct tgcggcccca gcggtggggt gcggtaaaac gaagaaacag caagaatgcg
      361 gctttctgga atatcgccta atgctttcag ggcagcattg acctgctcca gcggagaggc
      421 cagattgctg cctatggcaa tatacgccac tgtcatgcgg taccctcacg acgcggtgcg
      481 cgtttgcgtg gacgacgagt acgacgacgc ggtgacggtt cttcatccag ttcgttaagc
      541 atcccttttt ggtctggtgg cgcggaaacc tggaactcac cccaccattt caccagacgt
      601 tgcagttcag cgttacgctc aacttcggct cgtaaggcca acaggtcgta tgccgcacgg
      661 aacttaggat gctccagcag tttccatgcg cgttttccct gacgacggga catacgcaac
      721 tgcaactgcc agatatcgcg ggttagcgtg gtcagccgtt tcgggatcgc cagtgaacgg
      781 caggcttcgt ccagcacgtc gttcatcgcc agtgcgaaag cgtcgtgata ggtcaggccg
      841 ctttcctggg cgatcttctg tgccgtctcc agcaatggat accagaacat ggcggcaaac
      901 aggaacgccg ggttcacgcg catatcgtta tggatacgcg tatcggtatt cttcagtacc
      961 tgctcaataa tgcgttccat cgggctgtcg ccattttccg tgaagtagcg ggtaatggtc
     1021 gggaacagcg gctggaacag atgatattca cacagcagct tataggtttc gtaaccgtag
     1081 cccgcttgca acagtttcag cgattcttca aacagacgtg ccggtgggat atcgttcagt
     1141 aaggttgcca ggcgtgggat cggctcggcg gtttccgggc tgatgcgcat gcccaatttg
     1201 gcggcaaaac gaaccgcgcg gagcatacgt accggatctt cacggtagcg cgtttccggg
     1261 ttaccaatca gacggataac gccgtctttc agatccttca tgccgccaac gtaatcacgg
     1321 acggtaaaat ctgccacgct gtaatacagg ctgttgatag tgaaatcgcg gcgctgggcg
     1381 tcttcttcga tggagccgaa aatgttgtcg cgtagcaaca taccgttttg cccgcgttgg
     1441 gaggtcgtgc ggtcgctgac gttaccttcg tgatgtccac ggaaggtcgc aacttcgata
     1501 atctccgggc caaacatcac atgagccaga cggaaacggc gacccaccag gcggcagtta
     1561 cggaacagtt tgcgcacctg ctctggcgtg gcgttggtgg tcacgtcaaa atctttcggc
     1621 tttttgccaa gtaacaggtc gcgcacgccg ccgccaacca gccaggcttc gtatcccgct
     1681 ttattgagcc tgtacattac cttcagggca ttttcactga tatctttgcg ggaaatagca
     1741 tgctgctcac gcgggatcac cgtcacctgt ggacgggcga ctgcctgttc agcctcgctt
     1801 tcctcgcggc ttagcacctt gcggcaaaaa ttagcgactc gggtaaaaat agtacacctc
     1861 ggtagtgtca aacatcattc aggacaaaaa aatagcggct aatcatagct cagcatgacg
     1921 catttgagaa tgttgaattt acaattgccg actcaggcac ggcggtcagc gtccagtttt
     1981 tgacggctga ctgaaggatt tgctcgacgc tgaaatcctg ccagtgtgtt tctacctgct
     2041 gacccagaaa atgaagtgcc gcgattaaca ccgggcgcgg atcgcctttc ggcaacgcag
     2101 gcgcatgatt ctgcttggaa agtttagcgc cttgtggatt aagcgccagc ggtaaatgga
     2161 tgtaatctgg cactttccag ccaaaaagct ggtacagcga gatttgccgc accgtcggtt
     2221 cgatcaaatc tgccccgcgg acaatttcgc tcacgccctg gaagtgatca tccaccacca
     2281 ccgccaggtt ataggcaaac aaaccatcac ggcgatgaat aataaaatct tcccgtgcca
     2341 gtttttcgtc ggcgtgaata atgcctcgca gcagatcagt aaactgggtg accggatgct
     2401 gctggcggat acgtactgcg gcgttgtctg gtccatgatg caaatcccgg caatgaccgt
     2461 cgtaaatacc gccaatgctt tgaatacgcg cacgcgtgca ggtgcagtaa taacttagtc
     2521 cttgctcatg taaccaggcg agtgcttcgc gataggcgtg gtgacgttgc gattgccaga
     2581 gaacgtcgcc gtcccagtac aggccgtaat gttccagctg gcgcaggata gtttctgcgg
     2641 caccgggaac ttcacgaggc gggtcgatat cttcaatgcg taccagccag cgaccttgcc
     2701 gggcgcgagc ctgtaaatag ctgccaagcg cggcaatcag agagccaaaa tgaagctcgc
     2761 cggaaggaga gggggcgaag cgaccaatat agtgtgtgtc tgtcatctct ttgaacaaaa
     2821 aataaggcgg gagcatttcc cgcctgtggt aaacgtgatg gaacggctgt aattagccag
     2881 ccatctgttt ttcgcgaatt tcagccagcg ttttgcagtc gatgcacaga tcggctgtcg
     2941 ggcgcgcttc cagacggcga ataccgattt caacaccgca ggattcgcag tagccgaaat
     3001 cttcgtcttc cacttttttc agcgtcttat cgatcttttt gatcagctta cgctcgcgat
     3061 cgcggttacg cagttcgagg ctgaactctt cttcctgggc tgcacggtct accgggtccg
     3121 ggaagttggc tgcttcatcc tgcatatgtg taacggtgcg atcgacttca tccctgagtt
     3181 gattacgcca tgcttccaga atacgacgga agtgcgccag ctgggcttca ttcatatact
     3241 cttcgcccgg cttctcctga tatggttcca ccccagcgat ggcgagaata ctcagggacg
     3301 atgttttacg gttttgccct tcttgcatgt tgcttctcct taacacgcac tatcgatccc
     3361 catgttcggg ggaaaaatga ggccgctata aatagcagat gcttttccgg atagcaatta
     3421 tctaaacgta acacttgaca actgtgtgag gaaaagcgta tttgcgcacg cgaccagaat
     3481 gtaaattaac cagttactta ctctactaca atgtaaccgg tagtgatttt ttaagagcca
     3541 tgcctttggc agaaagttcc gctttgtaag ccagaatttc tacccccctc tgttgagctt
     3601 ctgacaatag ttgcgcgtat ttctcatcga tgtggcgcgc gggtgaaaac cgtgtaatgg
     3661 ctgaatgcag cacggcgaaa aagataaccg cacgctggcc ttcagccgct acgctcatca
     3721 actcccgaag gtgtttctga cctcgttcag tgaccgcatc gggaaaatat ccctgttcgt
     3781 tctccgctaa cgtaaccgat ttcacttcaa tatagcagtc tggacgcgaa tccgcctgca
     3841 acataaagtc aatacggctg cgttcggcgc cgtattttac ttcgcttttc agcgagctat
     3901 agcctgccag ctctgaaatt gattcattaa ggatagcctc tttcgtcaac ctgttagccc
     3961 aaagcgtatt gacgcaaata attgcgccgc tctggctttg agttaattcc caggtgtgtg
     4021 ggtatttccg tttggtgttg tctgaagtcg aataccagac ggtatcgcca ggcgttgcac
     4081 aaccggtcat cgcacccgta ttcgggcagt gtagcgttaa ttcgcgacca tcgggtgtga
     4141 tcacatcggc taaaaaacgt ttgtaacgct gaattagcgt cgcgcgctgt agagggggag
     4201 aaaattccat tgcgacaatc cttgttattg cgttagcgcc cagcgtttta gcggcgtgta
     4261 gcgtgtgcgt ccacgggcaa acgaggaggc gtaaagggtg aactccgtca ccgtatacga
     4321 ccagttaaaa cctggcggcg ggattgtcac cgcctcgctg gcgtcgcgca ataaggtaat
     4381 atgtggatga aacggacgat tgctttgaaa acaaccgctg cgggcagcct gtgagcggag
     4441 catattcgcc agctggatta agccgcgagg cggctgtcgc atccctaacc acaccacacg
     4501 cgaacgcagc cattgaccgg cgtcatcaag cgtgagtgtg aaaccaggtt gacgaatccg
     4561 tccggctaaa agagaaagcg ccttctcttt ctctgcgctc acttcgccta aaaatgccag
     4621 agtcagatgc aaattatcgg cggcgaccgg acgtcccgcc tcaggtggga agtgtgtggc
     4681 gcgccagcgg ataatctgtt cgcggatttc tgcaggtaag tcgatagcaa agaacagccg
     4741 ttgcggttca gacatgtgag gcactcggtt atgaattacc gcgatgctac aatgtggcgc
     4801 gaagaatgtt aaccctctgg agcgttttgt gtcgtcgttg cccgttgctg ccgtcttacc
     4861 tgagttactt gctgccctcg atggtgcatc gcaggtgtta ttaagtgcgc cgaccggggc
     4921 cgggaaatca acctggctgc cgctgcaact gctggcgcat cccggcatta acgggaaaat
     4981 tatcctcctg gagccgcgtc gtctggcggc gcgtaacgtc gcgcaacgac tggcggagct
     5041 gcttaacgaa aagccaggcg ataccgttgg ctaccggatg cgtgcgcaaa actgcgtcgg
     5101 gccgaatacc cgcctggaag tagttaccga aggcgtgctg acgcggatga tccagcgtga
     5161 cccggaactg agtggtgttg gattggtgat cctcgatgag tttcatgagc gcagcttgca
     5221 ggcggatttg gcgttggcgc tgttactcga tgtgcaacaa ggtctgcgtg atgaccttaa
     5281 actgctgatt atgtcggcta cgctggacaa cgaccgcttg cagcaaatgc tgccaggagc
     5341 gcccgtcgtc atctcagaag ggcgctcgtt tccggttgaa cgccgttatt taccgctgcc
     5401 cgcgcatcag cgttttgacg aagccgttgc ggtagccacc gccgaaatgc tgcgtcagga
     5461 aagtggatca ttactgttat ttttacctgg cgtcggagaa attcagcgcg tgcaggaaca
     5521 actggcttcg cgcatcggca gtgatgtgtt gctctgcccg ctgtatggcg cgttgtcgct
     5581 gaacgatcag cgaaaagcga tccttccggc accgcaagga atgcgcaaag tggtgctggc
     5641 gaccaatatt gctgaaacca gtttaaccat cgaaggtatt cgtctggtgg tggattgtgc
     5701 ccaggaaagg ggggcgcatt ttgatccgcg tacagggctt acgcggttga ttactcaacg
     5761 cgttagtcag gcgtcgatga cgcaacgtgc cgggcgtgcc gggcgtctgg agccgggtat
     5821 ctgcctgcat ttaatcgcca aagaacaagc agaacgcgct gccgcacaaa gtgaaccgga
     5881 gatcttacaa agcgatcttt ccggtttgct gatggaatta ctgcaatggg gatgcagcga
     5941 tccggcgcag atgagctggc tggatcaacc gccaacggtg aatctactgg ccgcgaaacg
     6001 cctgttacgg atgctggggg cgctggacgg tgaacggctt agtgcgcaag ggcaaaaaat
     6061 ggcaacgctg ggtaacgatc cgcgtttagc ggcaatgctg gttagcgcga agaacgacga
     6121 cgaagctgct accgcggcaa aaattgccgc cattctcgaa gagccgccac ggatgggcaa
     6181 tagtgacctg ggcgtggcgt tttcgcgcaa tcaacccgcc tggcagcaac gtagtcagca
     6241 actgttaaaa cgcttaaacg tacgcggcgg tgaggcagac agttcgctta tcgcgccgct
     6301 acttgccaga gcgtatgccg atcgcattgc tcgtcgccgt gggcaagatg aacgctatca
     6361 actggcgaac ggcatggggg cgatgctcga tgccgacgat gcgctaagcc gccacgaatg
     6421 gttgatcgca ccgttattat tgcagggcag cgcctcaccg gatgcacgga ttttactggc
     6481 gctgccggtc gatattgatg agttagtaca acgctgcccg cagctggtac agcagtctga
     6541 caccgtggag tgggatgacg cgcaaggtac gctgaaagcc tggcgtcggc tgcaaattgg
     6601 tcagttgatg gttaaagtgc aaccgctggc gaaaccctcg gaagacgagt tgcatcaggc
     6661 gatgcttaac ggcattcgtg ataaaggttt aagcgtgctc aactggacgg cggaagcgga
     6721 acagctacgc ttgcgtttgt tatgcgccgc aaagtggttg ccggaatatg actggccagc
     6781 ggttgatgat gaaagtttgt tggcgacgct ggaaacgtgg ctactgccgc atatgacagg
     6841 cgtacattcg ctacgcggcc tgaaatcact cgatatttat caggcactgc gtggattact
     6901 tgattgggta atgcagcaac gtctggatag tgaattgcct gcgcattaca ctgtgccgac
     6961 gggaagccgg atcgccattc gttaccatga agataacccg cccgcgctgg cggtgagaat
     7021 gcaggagatg tttggtgagg ccactaatcc gactatcgcc caggggcgcg taccgttggt
     7081 gctggagttg ctttcccctg cccaaaggcc gctgcaaatc acgcgtgatt tgagcgcctt
     7141 ctggaaagga gcgtaccgtg aggtgcaaaa agagatgaaa gggcgttatc ccaaacatgt
     7201 ctggccggac gacccggcaa acaccgcacc gacgcgacga acgaaaaagt attcgtgaag
     7261 accaaagcaa atttgagaga tatcttcttc tgtcttgcaa cagaagaaca gaaaatcggg
     7321 cttttgcgcc tgaatattgc ggagaaaaag catggccggg aatgaccgcg agccaattgg
     7381 acgcaaaggg aaaccgacgc gtccggtcaa acaaaaggta agccgtcgtc gttacgaaga
     7441 tgacgatgat tacgacgatt atgatgacta tgaggatgaa gaaccgatgc cgcgcaaagg
     7501 taagggcaaa gggcgtaagc ctcgtggcaa acgcggctgg ctatggctgc tgctaaaact
     7561 ggctatcatt tttgccgtga tgatcgccat ttacggcgtt tatctcgatc aaaaaattcg
     7621 cagccgtatt gatggcaagg tctggcaatt gcccgcggca gtttatggtc gaatggtcaa
     7681 ccttgagcca gacatgacca tcagcaagaa cgagatggtg aagctgctgg aggcgaccca
     7741 gtatcgccag gtgtcgaaaa tgacccgtcc tggtgagttt accgtacagg ccaacagcat
     7801 tgagatgatc cgccgtccgt ttgatttccc ggacagcaaa gaagggcagg tgcgcgcgcg
     7861 tctgaccttt gatggcgatc atctggcgac gatcgtcaat atggagaaca accgtcagtt
     7921 cggtttcttc cgtcttgatc cgcgcctgat caccatgatt tcttcgccaa acggtgagca
     7981 gcgtctgttt gtgccgcgca gtggtttccc ggatttgctg gtggatactt tgctggcgac
     8041 agaagaccgt catttctacg agcatgatgg gatcagtctc tactcaatcg gacgtgcggt
     8101 gctggcaaac ctgactgccg gacgcacggt tcagggggcg agtacgctga cgcaacagtt
     8161 ggttaaaaac ctgttcctct ccagcgagcg ttcttactgg cgtaaagcga acgaagccta
     8221 catggcgctg atcatggacg cgcgttacag caaagaccgt attcttgagc tgtatatgaa
     8281 cgaggtgtat ctcggtcaga gcggcgacaa cgaaatccgc ggcttcccgc tggcgagctt
     8341 gtattacttt ggtcgcccgg tagaagaatt gagcctcgat cagcaggcgc tgttggtggg
     8401 tatggtgaaa ggggcgtcca tctacaaccc gtggcgtaac ccgaaactgg cgctggagcg
     8461 acgtaacctg gtactacgtt tgctgcaaca gcaacaaatt attgatcaag aactctacga
     8521 catgctgagt gcgcgtccgc tgggtgttca gccgcgcggt ggggtgatct ctcctcagcc
     8581 agcctttatg cagctggtgc gtcaggagct gcaggcgaaa ttaggcgata aggtaaaaga
     8641 tctctccggc gtgaagatct tcactacctt tgactcggtg gcccaggacg cggcagaaaa
     8701 agccgccgtg gaaggcattc cggcactgaa gaaacagcgt aagttgagcg atcttgaaac
     8761 cgcgattgtg gttgttgacc gctttagcgg tgaagttcgt gcgatggtcg gtggttctga
     8821 gccgcagttt gcgggctaca accgtgcgat gcaggcgcgt cgttcgattg gttcccttgc
     8881 aaaaccagcg acttatctaa cggccttaag ccagccgaaa atctatcgtt tgaatacgtg
     8941 gattgccgat gcgccaatcg cgctgcgtca gccgaatggc caggtctggt caccgcagaa
     9001 cgatgatcgt cgttatagcg aaagtggcag ggtgatgctg gtagatgcgt tgacccgttc
     9061 gatgaacgtg ccgacggtga atctgggcat ggcgttgggg ctgcctgcgg ttacggagac
     9121 ctggattaaa ctgggtgtgc cgaaagatca actgaatcca gttccagcaa tgttgttggg
     9181 ggcgttgaac ttaacgccaa tcgaagtggc gcaggccttc cagactatcg ccagcggtgg
     9241 taatcgtgct ccgctttccg cgctacgttc agtcatcgca gaagacggca aggtgttgta
     9301 tcagagcttc ccgcaggcgg aacgcgctgt tccggcgcag gcggcgtatc tgacactatg
     9361 gaccatgcag caggttgtac aacgcggtac gggacgtcag cttggggcga aatacccgaa
     9421 cctgcatctg gcagggaaaa cagggactac caacaataac gtagatacct ggtttgcggg
     9481 cattgatggg agcacggtga ccatcacctg ggtcggccgt gataacaacc agccgaccaa
     9541 actgtatggt gccagcgggg caatgtcgat ttatcagcgt tatctggcta accagacgcc
     9601 aacgccgctg aatcttgttc cgccagaaga tattgcagat atgggcgtgg actacgacgg
     9661 caactttgtt tgcagcggtg gtatgcgtgt cttgccggtc tggaccagcg atccgcaatc
     9721 gctgtgccag cagagtgaga tgcagcagca accgtcaggc aatccgtttg atcagtcttc
     9781 tcagccgcag caacagccgc aacagcaacc tgctcagcaa gagcagaaag acagcgacgg
     9841 tgtagccggt tggattaaag atatgtttgg gagtaattaa cgtctaagcg tgatataccg
     9901 gatggcgagc tgccatccgg taaaataaca tcccctccaa agtattaacc ctttcttttc
     9961 atctgattgt ttattgaccc ttcaggaatg cgcagattgc gtaccgcttg cgaacccgcc
    10021 agcgtttcga atattatctc atctttataa taatcattct cgtttacgtt atcattcact
    10081 ttacaccaga gaaataccaa tggcaccttc caaaactgct cagccaaaac actcactgcg
    10141 taaaatcgca gttgtagtag ccacagcggt tagcggcatg tctgtttatg cacaggcagc
    10201 ggttgaaccg aaagaagaca ctatcaccgt taccgctgca cctgcgccgc aagaaagcgc
    10261 atgggggccg gctgcaacta ttgcggcgcg acagtcagct accggcacta aaaccgatac
    10321 gccgattcaa aaagtacctc aatctatttc tgtcgtcacc gctgaagaga tggcgctgca
    10381 tcagcctaag tcggtgaaag aagcgcttag ctatacccct ggcgttgctg tgggaacccg
    10441 tggcgcatct aatacttatg attacctgat cattcgcgga tttgcggcag acggccaaag
    10501 ccagaataac tatctgaatg gcctgaagtt gcagggcaac ttctataacg atgcggtcat
    10561 cgacccgtat atgctggaac gtgctgaaat tatgcgtggc ccggtttccg tgctttacgg
    10621 taaaagcagt cctggcggtc tgttgaatat ggtcagcaag cgtccgacca ctgaaccgtt
    10681 gaaagaagtt cagtttaagg ccgggactga cagcctgttc cagaccggtt ttgacttcag
    10741 cgatgcactg gatgatgacg gcgtttattc ttatcgcctg accggtcttg cgcgttctgc
    10801 caatgcccag cagaaagggt cagaagagca gcgttatgct attgcaccgg cgttcacctg
    10861 gcgtccggac gataaaacca atttcacctt cctttcttac ttccagaacg agccggaaac
    10921 tggttattac ggctggctgc cgaaagaggg gaccgttgag ccgctgccaa acggtaagcg
    10981 tctgccgaca gactttaacg aaggggcgaa gaacaacacc tattctcgta acgagaagat
    11041 gattggttat agcttcgacc acgaatttaa cgacaccttt actgtgcgtc agaacctgcg
    11101 ctttgcgcaa aataaagttt cgcaaaagag cgtatatggc tacggtatgt gttcagatcc
    11161 gctgtatacc aaagaccagg aggcactcaa ggcgagtcca tgtttaagta tcccgcagtc
    11221 tcaatggggc catacactga ctcgtcagta cgttatcgat aatgagaagt tagaaaactt
    11281 ctctgttgat actcagttgc aaagcaaatt cgcaaccggt tctgtggatc acactctgct
    11341 gactggcgtt gactttatgc gtatgcgtaa tgacatcgac tcctggttcg gatatgccgg
    11401 ttcagttgca ccatctgata tctataattt agaccgtagt gactttgatt ttggcgctca
    11461 cccgaatccg tctggtccat accgcgttct gctgaaacag aaacaaaccg gcctgtatgt
    11521 tcaggatcag gcgcagtggg ataaagtgct ggttacgctt ggcggtcgct atgactgggc
    11581 cgatcagtca tctttcaacc gtgattacgg taataaatct gaacgtgacg acaaagagtt
    11641 cacctggcgt ggtggtgtaa actacctgtt cgacaacggc gtaacgcctt acttcagcta
    11701 cagtgaatca tttgaacccg cttcacagac tgatgcgaac ggtgatctgt ttgcaccttc
    11761 gaaaggcaaa cagtatgaag tcggcgtgaa atatgtgcca gaagatcgcc caattgtggt
    11821 gactggcgca ctgtatcagc ttaccaaaac taataacctg atggcggatc cgaacggttc
    11881 actgttctcg gttgaaggcg gtgagattcg tgcgcgtggc gtagagctgg aagcgaaagc
    11941 ggcactgtcg gcgagtgtta acgtagtcgg ttcttatact tacaccgatg cggaatacac
    12001 taccgatact acctacaaag gcaatacgcc tgcacaggtg ccaaaacaca tggcttcgct
    12061 gtgggctgac tataccttct ttgacggtcc gctttcaggt ctgacgctgg gcaccggtgg
    12121 tcgttatact ggctccagct atggtgatcc ggctaactcc tttaaagtgg gaagttatac
    12181 ggtcgtggat gcgttagtgc gttatgatct ggcgcgagtc ggcatggcgg gctccaacgt
    12241 ggcgctgcat gtcaacaacc tgtttgatcg tgaatacgtc gccagctgct ttaacactta
    12301 tggttgcttc tggggcgcag aacgtcaggt cgttgcaacc gcaaccttcc gtttctaatt
    12361 tctcttttgg ggcacggatt tccgtgcccg tttcacaagt tggctgttat gcaggaatac
    12421 acgaatcatt ccgataccac ttttgcactg cgtaatatct cctttcgtgt gcccgggcgc
    12481 acgcttttgc atccgttgtc gttaaccttt cctgccggga aagtgactgg tctgattggt
    12541 cacaacggtt ctggtaaatc cactctgctc aaaatgcttg gtcgtcacca gccaccgtcg
    12601 gaaggggaga ttcttcttga tgcccaaccg ctggaaagct ggagcagcaa agcgtttgcc
    12661 cgcaaagtgg cttatttacc gcagcagctt cctccggcag aagggatgac cgtgcgtgaa
    12721 ctggtggcga ttggtcgtta cccgtggcat ggcgcactag ggcgctttgg tgcggcagat
    12781 cgcgaaaagg tcgaggaagc tatctcgctg gttggcttaa aaccgctggc gcatcggctg
    12841 gtcgatagcc tttctggcgg cgaacgtcag cgggcgtgga tcgccatgct ggtggcgcag
    12901 gatagccgct gtctgttgct cgacgaaccg acttcggcgc tggatatcgc ccaccaggtt
    12961 gatgtactgg cgctggtgca tcgtttaagt caggagcgtg gcctgacggt cattgccgtg
    13021 ctgcacgata tcaatatggc ggcgcgctac tgtgattatc tggtcgccct gcgcggcggt
    13081 gaaatgattg ctcagggaac gcctgcggaa attatgcgcg gcgaaaccct cgaaatgatt
    13141 tatggcatcc cgatgggtat tctgccgcat ccggcgggtg ctgcacctgt gagttttgtt
    13201 tattgatgag cggcttacct cttatttcgc gccgccgact gttaacggcg atggcgcttt
    13261 ctccgttctt atggcagatg aataccgccc acgcggcagt tattgatccc aatcgtattg
    13321 tggcactgga gtggttgccg gtggagttac tgctggcgct cggcatcgtg ccttacggcg
    13381 tggcggacac catcaactat cgcctgtggg tcagcgaacc gccattgccg gactcagtga
    13441 ttgacgttgg tttgcgtaca gaacccaacc ttgaactgct gaccgaaatg aaaccgtcgt
    13501 ttatggtctg gtctgcagga tatggccctt caccagaaat gctggcacgt attgcgccgg
    13561 ggcgcggatt taacttcagc gacggcaaac agccactggc gatggcgcgt aaatcactga
    13621 cggaaatggc agatttactt aacctgcaaa gcgcagcgga aacgcattta acgcactatg
    13681 aagactttat ccgcagcatg aaaccccgct ttgtgaagcg tggtgcacgc ccgttattgc
    13741 tgacgacgct tatcgatccg cgccatatgt tggtcttcgg tccaaacagc ttgttccagg
    13801 aaattctcga tgagtacggc atcccaaatg cctggcaagg ggaaaccaac ttctggggca
    13861 gtaccgccgt cagtatcgat cgtctggcag cgtataaaga cgttgatgtg ctctgttttg
    13921 atcacgacaa cagcaaagac atggatgcgc taatggcaac cccgctgtgg caggccatgc
    13981 cgtttgttcg cgccggacgc tttcagcgcg ttccggcggt ctggttctat ggtgcgacgc
    14041 tttctgcaat gcactttgtg cgtattctgg ataacgccat cggaggtaaa gcgtgagtaa
    14101 acgaattgcg ctttttccgg tgttattgct ggcgctgctg gtggttgctg ctgcggcgtt
    14161 gacctggatg aacttctcgc aggcgctgcc gcgcagccag tgggcgcagg ccgcctggtc
    14221 gccggatatt gacgtcatcg agcagatgat ttttcactac agcttgttgc cgcgtctggc
    14281 gatttcgctg ctggtgggcg cgggcctggg gctggtgggc gtgctgtttc agcaagtgct
    14341 gcgtaacccg ctggcggaac cgacgacatt gggtgttgca acaggcgcgc aactggggat
    14401 taccgtcacc acgctctggg caatccccgg cgcgatggcg agccagtttg ctgcgctggc
    14461 aggggcttgt gttgttggct taatcgtctt tggcgtcgcg tgggggaaac gtctttcgcc
    14521 ggtaacgctg atcctcgcgg ggctggtagt gagcctttat tgcggcgcaa tcaatcagtt
    14581 actggttatc ttccatcatg accaactgca aagcatgttc ctgtggagca ccggaacgct
    14641 gacgcaaacc gactggggcg gcgttgaacg tttatggccg cagctgctgg gcggcgtgat
    14701 gctgacgtta ttgctacttc gcccgttaac tctgatgggg cttgatgatg gcgtggcgcg
    14761 caatctcggg ctggccttgt cgctcgctcg tctggcggcg ttgtcgctgg cgattgtcat
    14821 cagtgcgctg ctggtgaacg cggtggggat tatcggcttt accgggttgt tcgcgccact
    14881 gctggcgaaa atgctgggga cgcggcgttt gttgccacgg ttgatgctgg catcgctgat
    14941 tggtgcgtta attctgtggc tttctgatca aatcatcctc tggctgactc gcgtgtggat
    15001 ggaagtgtcc accggttcgg tcactgcgtt gatcggtgcg ccgctgctac tgtggttgct
    15061 gccgcgttta cgcagcatta gcgcgccgga tatgaaggtc aacgatcgtg tcgcgactga
    15121 acgccaacac gtgctggcgt ttgccctcgc gggcggcgtg ctgctgttga tggctgtggt
    15181 ggtggcgctg tcgtttggtc gtgatgcgca cggctggacg tgggcgagcg gggcgttgct
    15241 cgaggatttg atgccctggc gctggccgcg aattatggct gcgctgtttg cgggcgtcat
    15301 gctggcggtg gcgggctgta ttattcagcg gctgaccgga aacccgatgg caagcccgga
    15361 agtgctgggg attagctccg gcgcggcgtt tggcgtggtg ttaatgctgt ttctggtgcc
    15421 gggtaatgcc tttggctggc tgttacccgc aggcagtctc ggggcggcgg tgacgctgtt
    15481 gatcattatg atcgccgccg gacgcggtgg attttcccca caccgcatgt tactggcggg
    15541 gatggcgtta agtaccgcgt tcaccatgct tttgatgatg ttgcaggcaa gtggtgaccc
    15601 gcgaatggcg caagttctga cctggatttc cggttcgacc tacaacgcga ccgatgcgca
    15661 ggtctggcgt accggaattg tgatggtgat tttgctggcg attaccccgc tgtgccgccg
    15721 ctggctgacc attttaccgc tgggtggtga taccgcacga gccgtgggga tggcgctgac
    15781 gccgacgcga attgcgctgc tgctgttagc ggcttgcctg acggcgaccg cgacaatgac
    15841 cattggaccg ttgagttttg ttggtttaat ggcaccgcat attgcgcgga tgatgggctt
    15901 tcgacggacg atgccacaca tcgtaatttc ggcgctggtg ggtggtttac tgctggtgtt
    15961 cgctgactgg tgtgggcgga tggtgctgtt tccattccag atccccgcgg ggctgctgtc
    16021 gacctttatc ggcgcgccat attttatcta tttgttgaga aagcagagcc gttaattgaa
    16081 tgcctcgccc ggtgggcgag gcaaaatcat cacaacttcg caaacacccg acgtgcagca
    16141 tcgatggtgt tattgatatc ttccatgctg tgcgccacag acataaagcc cgcttcaaac
    16201 gctgacggtg ccagataaac accttcgtcc agcatcatat ggaagaaacg cttaaagcgt
    16261 tccacgtcac aggccatcac atcctgatag cacgtcacgg actcggcgtc ggtaaagaaa
    16321 ataccgaaca tgccgccaac gtggtttacc accagcggaa ttccggcttc ttctgccgct
    16381 tcccgcagac cttctgccag acgtgttgtc agctcatcca gcgtctcgtg aacgcccggc
    16441 tgtgcgactt cattcagaca ggcgaaacca gcagccatcg caatcgggtt accggaaagc
    16501 gtacccgcct gatagaccgg accggtcggg gccagcgcat ccattacatc acgacgacca
    16561 ccgaatgcgc ctaccggcat tccaccgccg atgattttgc cgaggcaggt gagatccggt
    16621 tccacaccgt aataatcctg tgcgccagct agcgctacgc ggaaaccggt catcacttca
    16681 tcgatgatca gcaacgcgcc aaattcgtcg cacagcgcgc gcagacctgg caggaactct
    16741 ggcagcggtg gaacgcagtt catattgcct gccaccggct cgacgataat acaggcaatc
    16801 tcttgcgggt attgctcaaa cgcggcgcgt acagaggcca gatcgttata agtacaggtt
    16861 aaggtatgtt tggcgaaatc tgccggaacg cccggcgaat ttggctggcc taacgtgagt
    16921 gcgccagaac cggctttcac cagcaggcag tcagcgtgac cgtggtaaca accttcaaat
    16981 ttaataattt tgtcgcgacc ggtaaaacca cgggccaggc ggatggcgct catcgtcgct
    17041 tcagtgccgg agttcaccat gcgcaccata tccatggtcg ggaccagttc ggtcaccagt
    17101 tgcgccattt tcacttccat ttcggttggt gcaccaaagc ttaaaccacg ctcggcggct
    17161 tcaatcacgg cattgcggat cgccggatgg ttatggccca gcaccatcgg cccccaggaa
    17221 ccgacataat cgatataggc tttgccatca acatcgtaca gataagcgcc gtccgctttt
    17281 tcgataaaca gtggagtgcc gcccacgcca gtaaaggcgc gaacagggga gttcacgccg
    17341 ccagggatca gctcgcgcgc tgcgctgtaa agattttcag acttactcat ggagggttcc
    17401 tgattcgtag aaaaagtgaa tggctgctat tctatgttat tcataacaag ttaaatactc
    17461 gtcaaacatc aggctgcttg tactggtgca atcctgattt cttagagtat aaaagttttg
    17521 tgcatttgaa acattacgct ttgcaaagga ttttcatgga acgtgcgagt aaaatgccgt
    17581 catcttattt gtatgaccaa taagtgatca ttggatgaaa actgatactc cctctttaga
    17641 aacaccgcag gccgcgcgcc tgcgacgcag acaactgatt cgccaacttc ttgagcgcga
    17701 taaaaccccg ttagccattt tgtttatggc ggcagtcgtc ggcacgcttg tcgggctggc
    17761 tgcggttgct tttgacaaag gtgtcgcttg gttgcagaac caacgtatgg gggcactggt
    17821 acatactgct gataattatc cgctactgtt aaccgtcgct tttctctgtt cggcggtgct
    17881 ggcgatgttc ggctactttt tggtgcgcaa atacgcgccg gaagcaggtg gttcggggat
    17941 cccggaaatt gaaggggcgc tggaagatca acgtcccgtt cgctggtggc gtgtgttgcc
    18001 ggtgaaattc tttggcgggc tggggacact cggcggaggc atggtgttgg ggcgcgaagg
    18061 gccaaccgtg cagataggcg gcaatatcgg gcggatggta ctcgatgttt tccgcctgaa
    18121 aggtgacgaa gctcgtcata cgctgttggc aaccggtgct gctgcggggc tggctgcggc
    18181 ctttaacgcg ccgctggcgg gtattttgtt tattatcgaa gagatgcgtc cgcagtttcg
    18241 ctatacgtta atttcgatta aagcggtatt tattggcgtc attatgtcga ccattatgta
    18301 ccggattttt aatcatgaag ttgcgttgat tgacgtcggt aaactttctg atgcgccgct
    18361 taatacgctg tggctttatc tgatcctcgg tattattttt ggcattttcg gccctatttt
    18421 taataaatgg gtgctgggga tgcaggattt gctgcaccgt gtgcacggtg gcaatattac
    18481 caaatgggta ctgatgggtg gtgcgattgg cggtctgtgt ggattgctgg ggtttgtggc
    18541 accagcaact tcgggcggcg gttttaacct gattcctatc gctaccgcgg ggaatttcag
    18601 catgggaatg ctggtgttta tcttcgtcgc gcgggtcatt accactttac tctgcttctc
    18661 ttccggcgcg ccgggcggta tttttgcccc gatgctggcg ctgggtactg tgctgggaac
    18721 cgctttcgga atggttgccg ttgagttgtt tccgcaatat caccttgagg cggggacgtt
    18781 tgctattgcc ggaatggggg cgttactggc ggcatctatt cgcgcgccgt taacggggat
    18841 cattctggtt ctggaaatga ccgataacta ccagctcatt ttgccaatga ttattaccgg
    18901 tcttggcgca acactattag cacaatttac cggtgggaaa ccgctatact cggcgattct
    18961 tgcgcgcacg ctggcaaaac aggaagctga gcaactggcg cgaagcaagg ccgcatcagc
    19021 ccgcgagaat acttgaacga aataccaggg tattagataa tggcgattat tattgggtta
    19081 gaatttgccc aattgccgat gtcgtttgga gcaaaatatg agtgatgacg tagcactgcc
    19141 gctggagttt accgacgcag cagccaacaa agttaaaagc ctgatcgctg acgaagataa
    19201 cccgaatctg aaattacgcg tgtatatcac cggtggcggt tgcagcggct tccagtatgg
    19261 tttcaccttt gatgatcagg tgaacgaagg cgatatgacc atcgaaaaac agggcgttgg
    19321 cctggtggtt gatccgatga gcctgcaata tctggtcggc ggttccgttg actataccga
    19381 aggtctggaa ggttctcgtt tcatcgtgac caacccgaac gcgaaaagca cctgcggttg
    19441 cggttcttcc tttagtatct aatctgctgt tcatgtgccg gataaactat ccggcacatc
    19501 ttctctcttc aacgaccatt ctcatccagc gcaaacgtcg gtagcttaag atgccaacga
    19561 atagccgcca gccgaatcaa tagcgtcacg accatgccca tcatactggc tgtttccagt
    19621 ggtacggaaa atgtgtaata agccgtagcg tggacaatac cgccgataat acaggcagtt
    19681 gcgtagattt ctgtacgtaa aatcatgggg atttcgcggg ccagtacatc acgaatgatc
    19741 ccgccgccaa cgccagtaat gacgcccata caaaccgcga ttaacggacc ggcttccgca
    19801 ttaaaggctt tattcacgcc aatgccgaca aacaccgcca gaccaacggc gtccagcacc
    19861 ggcaacatcc attttggtaa gcgtcttggc tggcgcacca gcacgatggt cagcatgctg
    19921 gtgaccattg caacgaccag atcggtggga tctttcaccc aaaataccgg gccgtgatcc
    19981 agcgccatgt cgcgaattgt tccgccgcct actgcggtaa ccacgcccaa taccagaaca
    20041 ccaaaagggt ccatacgcaa ttttccggct aacaaaacgc cagagatagc aaataccgct
    20101 gtgccgacta tatccagcca atagacgagc attgttcaat ccccactgag cacctgaaaa
    20161 ggtcaggcac taatctacct gtgaaagcgc attacagagc tgttgtgcag cgaggataat
    20221 acgtgggctt gcacgttcaa accagtcact cgtgagagga ataacgggaa ttttgagctg
    20281 ttcaccccag tattgtttga ttttaggaat ttggtccggt ccgcctgtaa tgacaatcgc
    20341 ctgtggcgag cgtgctaaca cctgttcgcg gctaacttgc ggccagggaa cccggctgtc
    20401 tttaaagatg ttttctccgc cacaaacttc gagtacctgg ttctgaatcg actcttttcc
    20461 actggtaaat ggcggattaa tgccgaattg cagaaaaaca cgttttttag gtttatcagc
    20521 atattgcgct ttcaattgcg cgtactgatc cagcagggat tgcgcggctt gttcggcctt
    20581 atccggttgc ggactccagg gggccagttg acgtaacgca ttggcaattt gttcaatgct
    20641 tgtcgcatcg acccacatca cttttattcc cagcgaagcc agctggtcaa cctgccgctc
    20701 ggcattacct ccacgccagg caatcaccag atcgggtttc agcgcgacaa tgcgttccag
    20761 attcatcccc tgccaggtgg aaacctgctc aatcttttgc gcttgtggag gatagtcgga
    20821 atagctgctg accccaaccg gcgtgatccc ggcggcaaag gcaagttcag tgttggcggg
    20881 agaaagcgtg atgacgcgcg gcgcggcatt gagccacagc ggcgcgagaa aagacagggc
    20941 gaccagcgcc ctgaacagtg acttagccat gtgccagttt ctgcaccagt gactcaacca
    21001 tcaggctgga ctgtttagcg gcaacagcca ggaactcatc gaagctaaga tgagactgtt
    21061 gatcggccac gtcggagatg gcgcgtacta cgacaaacgg gacgttgaaa ttgtggcaga
    21121 catgggcgat tgccgtcgct tccatctcta cagcaatggc ctgtgggaag ttgtggcgga
    21181 ttttcgccag accaacagaa ccgttgatga aagcgtcgcc gctaacaatc aggccacgta
    21241 cagcgttaag attcagttcg gcaatgcagg cctcagcggc agcgatcagt ttatcgtcag
    21301 ctttgaagcc tgccgggcag cctggtaact gaccgtattc ataaccaaat gccgtgacat
    21361 ccgcgtcgtg ataacgcgct tcgtccgaga caacgatatc gcccactttc agcgttggtg
    21421 ccaggccacc ggcagaaccg gtgttaataa tcacatctgg cttgcagtgt tccaacagca
    21481 aagtggcacc cagcgccgca gcgactttac cgatgcccga tttcagaagc gcaacctcgg
    21541 ttccattcag ttggccggta tagatttcgc aaccgcccag actgatagtt tgacggtttt
    21601 cgattttgtc acgcagcagc gtaacttctt cttccattgc accaatgatg ccgattttca
    21661 tagatttact cgcgataagc ccgatttgaa ggcatagttt accatgcgct tacggggaag
    21721 cgtatttctc acgcgggaga ggacatggca cagattgatt tccgaaaaaa aataaactgg
    21781 catcgtcgtt accgttcacc gcagggcgtt aaaaccgaac atgagattct gcggatcttc
    21841 gagagcgatc gcgggcgtat catcaactct ccggcaattc gtcgtctgca acaaaagacc
    21901 caggtttttc cactggagcg caatgccgcc gtgcgcacgc gtcttaccca ctcgatggaa
    21961 gtccagcagg tggggcgcta catcgccaaa gaaattttaa gccgtttgaa agagcttaaa
    22021 ttactggagg cgtatggtct cgatgaactg accgggccct ttgaaagcat tgttgagatg
    22081 tcatgcctga tgcacgatat cggcaatccg ccgtttggtc attttggcga agcggcgata
    22141 aatgactggt ttcgccagcg tttgtacccg gaagatgccg aaagccagcc tctgactgac
    22201 gatcgctgca gcgtggcggc actacgttta cgggacgggg aagaaccgct taacgcgctg
    22261 cggcgcaaga ttcgtcagga cttatgtcat tttgaaggta acgcacaggg tattcgtttg
    22321 gtgcatacat tgatgcggat gaatctcacc tgggcacagg ttggcggtat tttaaaatat
    22381 acccgtccgg cgtggtggcg tggcgaaacg cctgagacac atcactattt aatgaaaaag
    22441 ccgggctatt atctttccga agaagcctat attgcccggt tgcgtaaaga acttaatttg
    22501 gcgctttaca gtcgttttcc gttaacgtgg attatggaag ctgccgacga catctcctat
    22561 tgtgtggcag accttgaaga tgcggtagag aaaaggatat ttaccgttga gcagctttat
    22621 catcatttgc acgaggcgtg gggccagcat gagaaaggtt cgctcttttc gctggtggtt
    22681 gaaaatgcct gggaaaaatc acgctcaaat agtttaagcc gcagtacgga agatcagttt
    22741 tttatgtatt tacgggtaaa caccctaaat aaactggtac cctacgcggc acgacgattt
    22801 attgataatc tgcctgcgat tttcgccgga acgtttaatc atgcattatt ggaagatgcc
    22861 agcgaatgca gcgatcttct taagctatat aaaaatgtcg ctgtaaaaca tgtgtttagc
    22921 catccagatg tcgagcagct tgaattgcag ggctatcggg tcattagcgg attattagag
    22981 atttatcgtc ctttattaaa cctgccgtta tcagacttta ctgaactggt agaaaaagaa
    23041 cgggtgaaac gtttccctat tgaaacgcgc ttattccaca aactctcgac gcgccatcgg
    23101 ctggcctatg tcgaggctgt cagtaaatta ccgtcagatt ctcctgagtt tccgctatgg
    23161 gaatattatt accgttgccg cctgctgcag gattatatca gcggtatgac cgacctctat
    23221 gcgtgggatg aataccgacg tctgatggcc gtagaacaat aaccaggctt ttgtaaagac
    23281 gaacaataaa tttttacctt ttgcagaaac tttagttcgg aacttcgcgc tctaaaacga
    23341 atctgaagaa cacagcaatt ttgcgttatc tgttaatcga gactgaaata catgaaaaaa
    23401 accacattag cactgagtgc actggctctg agtttaggtt tggcgttatc tccgctctct
    23461 gcaacggcgg ctgagacttc ttcagcaacg acagcccagc agatgccaag ccttgcaccg
    23521 atgctcgaaa aggtgatgcc ttcagtggtc agcattaacg tagaaggtag cacaaccgtt
    23581 aatacgccgc gtatgccgcg taatttccag cagttcttcg gtgatgattc tccgttctgc
    23641 caggagggtt ctccgttcca gagctctccg ttctgccagg ggggcctggg cggtaatggc
    23701 ggcggccagc aacagaaatt catggcgctg ggttccggcg ttatcattga tgccgataaa
    23761 ggctatgtcg tcaccaacaa ccacgttgtt gataacgcga cggtgattaa agtccaactg
    23821 agcgatggtc gtaagttcga cgcgaagatg gttggcaaag atccgcgctc tgatatcgcg
    23881 ctgatccaga tccagaaccc gaaaaacctg accgcaatta agatggcgga ttctgatgcg
    23941 ctgcgcgtgg gtgattacac cgtagcaatt ggtaacccgt tcggcctggg cgagacagta
    24001 acttccggga ttgtctctgc gttgggacgt agcggcctga acgctgaaaa ctacgaaaac
    24061 ttcatccaga ctgatgcagc gatcaaccgg ggtaactcag gtggtgcgtt ggttaacctg
    24121 aacggcgaac tgattggtat caacaccgcg attctcgcac cggacggcgg aaacatcggt
    24181 atcggttttg ctatcccgag caacatggtg aaaaacctga cctcgcagat ggtggaatac
    24241 ggccaggtga aacgtggtga gttgggtatt atgggcactg agctgaactc cgatctggcg
    24301 aaagcgatga aagttgacgc ccagcgcggt gctttcgtaa gccaggttct gccgaattct
    24361 tccgccgcga aagcgggcat taaagcgggt gatgtgatca cctcactgaa cggtaagcca
    24421 atcagcagct ttgccgcact gcgtgctcag gtgggcacta tgccggtagg tagcaaactg
    24481 accctgggct tactgcgcga cgggaagcag gttaacgtga acctggaact tcagcagagc
    24541 agccagaatc aggttgattc cagcaccatc ttcaacggca ttgaaggcgc tgagatgagc
    24601 aacaaaggca aagatcaggg cgtggtggtg aacaacgtga aaacgggcac tccggctgcg
    24661 cagatcggcc tgaagaaagg tgatgtgatt attggcgcga accagcaggc agtgaaaaac
    24721 atcgctgaac tgcgtaaagt tctcgacagc aaaccgtctg tgctggcact gaacattcag
    24781 cgcggcgaca gcaccatcta cctgttaatg cagtaatctc cctcaacccc ttcctgaaaa
    24841 cgggaagggg ttctccttac aatctgtgaa cttcaccaca actccataca tcttcatcat
    24901 cctttgggca tttgcacaat gccgtacgtt acgtacttcc ttatgctaag ccgtgcataa
    24961 tggaggactt atggctggct ggcatcttga taccaaaatg gcgcaggata tcgtggcacg
    25021 taccatgcgc atcatcgata ccaatatcaa cgtaatggat gcccgcgggc gaattatcgg
    25081 cagcggcgat cgtgagcgta ttggtgaatt gcacgaagga gcattgctgg tactttcaca
    25141 aggacgagtc gtcgatatcg atgacgcggt ggcacgtcat ctgcacggtg tgcggcaggg
    25201 gattaatcta ccgttacggc tggaaggtga aattgtcggc gtaattggcc tgacaggtga
    25261 accagagaat ctgcgtaaat atggcgaact ggtctgcatg acggctgaaa tgatgctgga
    25321 acagtcgcgg ttgatgcacc tgttggcgca ggatagccgt ttgcgggaag aactggtgat
    25381 gaacctcatt caggctgagg agaatactcc cgcacttact gaatgggcgc aacggctggg
    25441 gatcgatctc aatcaaccgc gagtggtggc tattgttgag gtcgacagcg gtcagcttgg
    25501 cgtggacagc gcaatggcgg agttacagca actgcaaaat gcgctgacta cgcccgagcg
    25561 taataatctg gtggcgattg tctcgctaac cgaaatggtg gtgttgaaac cggcgttgaa
    25621 ctcttttggg cgctgggacg cagaagatca tcgtaagcga gttgaacaac tgattacccg
    25681 catgaaagag tatggccagc tgcgttttcg cgtttcgctg ggcaactatt ttaccggtcc
    25741 tggcagtatt gcccgatcct atcgtacggc gaaaacgacg atggtggtgg gtaaacagcg
    25801 gatgccagaa agtcgctgct atttttatca ggatctgatg ttacctgtgt tactcgacag
    25861 tttgcgtggc gactggcagg ccaacgaact ggcgcgaccg ctggcgcggc tgaaagcgat
    25921 ggacaataac ggcttgctgc ggcgaactct ggcggcgtgg tttcgccaca atgtgcaacc
    25981 gctggcaacg tcaaaggcgt tgtttattca tcgtaatact ctggaatatc ggcttaatcg
    26041 tatatcggaa ctgaccgggc ttgatttggg caattttgat gacaggttgc tgctgtatgt
    26101 ggcgttacaa ctggatgaag agcggtaggt cgattacaaa attacagccg ggtgagactt
    26161 tacccggccc acagaatggc attatttacg cgtcagtttt tccagatcgg cttcaatctc
    26221 gctgatctta ttggttacta ctgactccag gtgacgtaag tcgtcgagga tcttacgctt
    26281 aagatcaact tcgctgcggt cacgctggca gatttgatca agctcatcaa tgatataccg
    26341 tagattcggg ctaatttcct ggacttcttt ataaccctga cccacaccat cagcgacgac
    26401 cgttttacgc tgacgcgggt atttaaactt cacgctcttg gcgaaaaact cgcctttgtc
    26461 tttctggaaa tagattttca gaatatcgtt gttggcttcc tgccggaggc tgtaacgatc
    26521 aatttcttca ggattggtaa tacccagact tttcagattg tcgtacatag tgttacctca
    26581 aaatgagtca gtaaattgtg cttattttag catttggcct ggcccgcccg gctcgactgt
    26641 gatcgcagaa agcctttttc ttccagactt ctatcatggc gtaaaaatca aaaaattacc
    26701 tgctttattg tggtgatatt ccatgggatg gattagcaca tttatcttca ttttgcgaat
    26761 tacatgacag tccgaaacaa gatgaaaaag tatttaaaaa aaagggggaa aatgagtagg
    26821 acgaattatt ctgagccaga aagcaatcgg gcagcgtcgt gctgcccgtg tacatacttt
    26881 tagtcgatgg tacgcagcag ttcgttaata ccaactttac cgcgagtttt cgcgtcaact
    26941 ttcttaacga taactgcaca gtagaggctg tatttgccat ctttcgacgg cagattacct
    27001 gaaacaacca cagaccccgc cggaacgcga ccgtagtgga tttcgccggt ttcacggtca
    27061 taaatacggg tgctctgacc aatgtatacg cccatagaga tgacggaacc ttcttcgaca
    27121 atcacccctt caaccacttc agagcgtgcg ccgatgaagc agttatcttc aataatggtc
    27181 gggttagcct gcagcggctc cagcacgccg ccgataccca cgccaccgga aaggtggacg
    27241 tttttaccaa tctgcgcaca ggaaccgacg gtcgcccagg tatcaaccat ggtgccttca
    27301 tcaacatatg cgccgatgtt gacgtaagac ggcatcagca cggtgttacg ggcaatgaac
    27361 gcgccctgac gcactgccgc tggtggcacg acgcggaagc cttctttctg gaaacgtgct
    27421 tcatcgtagt tagcgaattt catcggcact ttgtcgaagt agcggctttc tgccccttcg
    27481 atcacctgat tatcattaat acggaaagag agcagcaccg cttttttcaa ccactgatgc
    27541 gtcacccact gaccgtcaat tttttccgct acacgcagtg cgccggaatc cagcagggcg
    27601 atcacctgat ttaccgcttc gcgggtaacg gtgtctgcat tggctggcgt gatctcggca
    27661 cggcgttcaa aagcggtttc aataatgttc tgtaactgct gcattgttaa actcttttca
    27721 tatcagtaaa cacatcaccc tttatcgttt ggattgaggg cctctgtcaa ccgctgatgc
    27781 acttcctgct gcaactcgtt attaagcgca cgccggtcag cggtggcaat tatgaataaa
    27841 tcttctactc gctcgccaat ggttgtaatt cgggcgccat gaagcgaaat tcccagatcg
    27901 gcaaaaattt tcccgactcg cgccagcagt ccaggttggt cgagggcgat cagttcgagg
    27961 aacgatttgc ggtcggtatg ggtcggcaaa aacgtcactt cggtttcaac agtaaaatgg
    28021 cgcaatttgg cgggttggcg acggggctgc ggtggctgcc agctactttg tatcagtact
    28081 tgctccagac caaaccgaat aacctcatga cgatctgcgg atagcgggct accatcgggt
    28141 tccagcacta taaaggtatc catcgccata ccgtcgcgag tggtgaaaat ttgtgcgtcg
    28201 tgaacactta aattgcggcg gtctaattcg gcacagacgg cggcaaacag ataggggcgg
    28261 tccgggctcc agataaaaat ctcggtgcct ccacgcgtag cctgcgggct aagcagtacc
    28321 agcggtttgc ttaaatcatg ctgtaataaa tggcgtgcgt gccaggccag ttgatttggg
    28381 ctatggcgga caaaatagtt agcgcgacag cgtgaccaaa tttggtgcag cgcctcttcg
    28441 tcgatgttat ccatgcgcag tagtgccagc gcctggagtt ggtgatggcg aacccgttcg
    28501 cgcatatccg gcgtgttttg cattccgcgt cgtagctgct tttcggtggc aaaatagagt
    28561 tcgcgcaaca gactttgctt ccagctattc cacagtgttt cgttggtggc gcaaatgtca
    28621 gccactgtca ggcataccag atagcgcaga cgattttccg tttgaacttc ttcggcaaac
    28681 tgcttgatga cttccgggtc ctgaatatcg cggcgctgag cggtcaccga catcagcaag
    28741 tgctggcgaa ccagccatgc gaccagctgt gtttcgcgtg agttcagccc gtggagttcg
    28801 gcaaaatgca ctacatcctg agcaccgaga atggagtggt cgccgccgcg tcctttggcg
    28861 atatcgtgaa acagcgcggc gatgaaaatc agctcagttg acggcaggcg cggccagacg
    28921 tccacacaca acggatggcg ctggcgcgtt tcttcactgg caaaactctc cagtttcagc
    28981 atcacgcgga tagtatgttc atccaccgtg taggcatgga acaggtcaaa ctgcatctgc
    29041 ccgacgatat gcgaccattg cggcatatac gcgccgagca cgctatggcg atgcattggc
    29101 aatagcccgc gccgcaccgc tccggggtga cgcagaatgc tcaaaaacag ttttcgcgct
    29161 tccggaatat tacacagagg ttgttgcaga tggcgacggg cgtggcgtaa ctggcgtagc
    29221 gtggtggagt aaatgccggt gatcgcactg ttgcgcacca tggtgtagaa catacgcaag
    29281 atggcttccg gctggcgcat aaatagtgtt tcatcacgca ggtcgatcag cgttccgcgt
    29341 agctgaaact catcgtcgat tggacgtggt ttttcgtcgg cgggaagggc gaggatggct
    29401 tcatcgaaca gttgcagcag catctggttg agttcactga cgcggcgtgt aacgcggaag
    29461 taatccttca tcatccgctc gaccggttcg ttaccttcac cactgtaatt caaacgctgg
    29521 gcgacgctaa gctggcgatc gaataacagg cgattatcgt aacgactgac gaccagatgc
    29581 agggcaaagc gaatgcgcca caatatatgc aggcattcgt ttaattccgc tctctccgca
    29641 gaggttaaga agccaaaccc taccatttca tccagcgatg ttgctccaaa atgacggcgg
    29701 gccacccatt gcagagtgtg gatatcgcgc aagccgccgg ggctactttt gatgtctggt
    29761 tcaaggttgt agctggtgcc gtggtaacgc tgatggcgct ggttctgttc ttcaactttc
    29821 gccgcgtaga acttgtcgga aggccagaat ccttcgctaa aaatatgttt ttgcagttcg
    29881 aggaacagcg caacatcgcc aattaataag cgggattcga ttaaattggt ggcgacggtt
    29941 aaatccgata acccttccag catgcactct tcaagcgtgc gcacgctatg acctacttcc
    30001 agctttacat cccagagcag cgttaacagc tcgcccactt tttgcgcctg atcgtccggg
    30061 agctttttac ggcttaaaat cagtaaatcg atgtcagaaa gtggatgcag ctcgccacga
    30121 ccgtagccac cgacggcgac caatgccagg tcggcaatct ggctgaatcc cgcttcaatc
    30181 cataatcgtt gaagaagctg gtcgataaac tcggtacgcg cctcaatcaa ctgttctgcg
    30241 gagataccat tgtcaaaggc atcacccagc caacgctgga aagtatcgat atgggctttt
    30301 atcccaccga cggttaattc atcacggggc caggcgcatg gattttgcgg ttgaccgtgc
    30361 agggtgggga gagcggtgtt tgcgtactgt tctggaaggg tattcattgt gcgccaccca
    30421 taagattaaa ttattgcatt aaaaaagccg gcattcgccg gcttcatttt attcgtcgtg
    30481 cgagattatc gccgggatgg tgtcatcctt gcgtagcgtc agaatttcgc agccgttatc
    30541 agtcaccaca atagtatgct catattgtgc agacaagctg cgatctttgg tttttaccgt
    30601 ccagccatct ttcatggtgc ggatctcttt tttacccgcg ttgaccattg gctcgatggt
    30661 gaacgtcatc ccaggtttca gtacgacgtt ggtttcacgg gagtcatagt gcagcacctg
    30721 cggttcttcg tggaagccgc gaccaatacc gtgtccacaa tattcacgaa cgacggagaa
    30781 accttctgct tcgacaaatt tctgaattgc cgcaccgatt tcgcgcagat taatgccagg
    30841 ttttaccatg cgtagcgcca ggtacaggct ttcttgcgtg atgcggcaca gacgttcgcc
    30901 catgatggtc ggcttaccga cgataaacat tttcgaggta tcgccgtgga aaccatcttt
    30961 gattacggtg acatcaatgt taacgatatc gccatctttc agcagcttgg cgtcgtccgg
    31021 gataccgtgg cacaccactt cattaataga gatgcaaacg gatttcggat agccgtgata
    31081 gccgaggcag gcagaaaccg cgtgttgttc attaacaatg taatcattac agatgcgatc
    31141 caactcgccg gtgctgacgc ccggtttaac atatggttcg atcatctcca gcacttcggc
    31201 agccagtcgg ccagcgacgc gcattttttc gatatcttct ggggtcttga ttgagatagc
    31261 cattaattct gtccatcagc gtcggtgata ccgacaatat atatgtaagt gccgtcaatg
    31321 gtatcacacc cgggcaaatt gagaatcatt ctgaatttcg ccaaacgtgc cactgaaggt
    31381 tttctataat agaaaattca acgtctgatg ctgtacacag cgccaacaat tattggtgtc
    31441 cacgacgtat ttgtggtata aagcgcgccg gacttccgat ccatttcgta tacacagact
    31501 ggacggaagc gacaatctca ctttgtgtaa caacacacac gtatcggcac atattccggg
    31561 gtgccctttg gggtcggtaa tatgggatac gtggaggcat aaccccaact tttatataga
    31621 ggttttaatc atggcaactg tttccatgcg cgacatgctc aaggctggtg ttcacttcgg
    31681 tcaccagacc cgttactgga acccgaaaat gaagccgttc atcttcggtg cgcgtaacaa
    31741 agttcacatc atcaaccttg agaaaactgt accgatgttc aacgaagctc tggctgaact
    31801 gaacaagatt gcttctcgca aaggtaaaat ccttttcgtt ggtactaaac gtgctgcaag
    31861 cgaagcggtg aaagacgctg ctctgagctg cgaccagttc ttcgtgaacc atcgctggct
    31921 gggcggtatg ctgactaact ggaaaaccgt tcgtcagtcc atcaaacgtc tgaaagacct
    31981 ggaaactcag tctcaggacg gtactttcga caagctgacc aagaaagaag cgctgatgcg
    32041 cactcgtgag ctggagaaac tggaaaacag cctgggcggt atcaaagaca tgggcggtct
    32101 gccggacgct ctgtttgtaa tcgatgctga ccacgaacac attgctatca aagaggcaaa
    32161 caacctgggt attccggtat ttgctatcgt tgataccaac tctgatccgg acggtgttga
    32221 cttcgttatc ccgggtaacg acgacgcaat ccgtgctgtg accctgtacc tgggcgctgt
    32281 tgctgcaacc gtacgtgaag gccgttctca ggatctggct tcccaggcgg aagaaagctt
    32341 cgtagaagct gagtaataag gcacgctcgt acgagccctt atagaccagg tagtacacgt
    32401 ttggttaggg ggcctgcata tggccccctt tttcactttt atatctgtgc ggtttaatgc
    32461 cgggcagatc acatctccga ggattttaga atggctgaaa ttaccgcatc cctggtaaaa
    32521 gagctgcgtg agcgtactgg cgcaggcatg atggattgca aaaaagcact gactgaagct
    32581 aacggcgaca tcgagctggc aatcgaaaac atgcgtaagt ccggtgctat taaagcagcg
    32641 aaaaaagcag gcaacgttgc tgctgacggc gtgatcaaaa ccaaaatcga cggcaactac
    32701 ggcatcattc tggaagttaa ctgccagact gacttcgttg caaaagacgc tggtttccag
    32761 gcgttcgcag acaaagttct ggacgcagct gttgctggca aaatcactga cgttgaagtt
    32821 ctgaaagcac agttcgaaga agaacgtgtt gcgctggtag cgaaaattgg tgaaaacatc
    32881 aacattcgcc gcgttgctgc gctggaaggc gacgttctgg gttcttatca gcacggtgcg
    32941 cgtatcggtg ttctggttgc tgctaaaggc gctgacgaag agctggttaa acacatcgct
    33001 atgcacgttg ctgcaagcaa gccagaattc atcaaaccag aagacgtatc cgctgaagtg
    33061 gtagaaaaag aataccaggt acagctggat atcgcgatgc agtctggtaa gccgaaagaa
    33121 atcgcagaga aaatggttga aggccgcatg aagaaattca ccggcgaagt ttctctgacc
    33181 ggtcagccgt tcgttatgga accaagcaaa actgttggtc agctgctgaa agagcataac
    33241 gctgaagtga ctggcttcat ccgcttcgaa gtgggtgaag gcatcgagaa agttgagact
    33301 gactttgcag cagaagttgc tgcgatgtcc aagcagtctt aattatcgaa aaggagccgc
    33361 ctgagggcgg cttctttttg tgcccatctt gtaaattcag ctaacccttg tggggctgcg
    33421 ctgaaaagcg acgtacaatg tcgctagtat taattcattt caatcgttga cagtctcagg
    33481 aaagaaacat ggctaccaat gcaaaacccg tctataaacg cattctgctt aagttgagtg
    33541 gcgaagctct gcagggcact gaaggcttcg gtattgatgc aagcatactg gatcgcatgg
    33601 ctcaggaaat caaagaactg gttgaactgg gtattcaggt tggtgtggtg attggtgggg
    33661 gtaacctgtt ccgtggcgct ggtctggcga aagcgggtat gaaccgcgtt gtgggcgacc
    33721 acatggggat gctggcgacc gtaatgaacg gcctggcaat gcgtgatgca ctgcaccgcg
    33781 cctatgtgaa cgctcgtctg atgtccgcta ttccattgaa tggcgtgtgc gacagctaca
    33841 gctgggcaga agctatcagc ctgttgcgca acaaccgtgt ggtgatcctc tccgccggta
    33901 caggtaaccc gttctttacc accgactcag cagcttgcct gcgtggtatc gaaattgaag
    33961 ccgatgtggt gctgaaagca accaaagttg acggcgtgtt tactgctgat ccggcgaaag
    34021 atccaaccgc aaccatgtac gagcaactga cttacagcga agtgctggaa aaagagctga
    34081 aagtcatgga cctggcggcc ttcacgcttg ctcgtgacca taaattaccg attcgtgttt
    34141 tcaacatgaa caaaccgggt gcgctgcgcc gtgtggtaat gggtgaaaaa gaagggactt
    34201 taatcacgga ataattcccg tgatggataa ataagggtaa gattccgcgt aagtatcgcg
    34261 ggggatctta cccgtaatat gtttaatcag ggctatactt agcacacttc cactgtgtgt
    34321 gactgtctgg tctgactgag acaagttttc aaggattcgt aacgtgatta gcgatatcag
    34381 aaaagatgct gaagtacgca tggacaaatg cgtagaagcg ttcaaaaccc aaatcagcaa
    34441 aatacgcacg ggtcgtgctt ctcccagcct gctggatggc attgtcgtgg aatattacgg
    34501 cacgccgacg ccgctgcgtc agctggcaag cgtaacggta gaagattccc gtactctgaa
    34561 aatcaacgtg tttgatcgtt caatgtctcc ggccgttgaa aaagcgatta tggcgtccga
    34621 tcttggcctg aacccgaact ctgcgggtag cgacatccgt gttccgctgc cgccgctgac
    34681 ggaagaacgt cgtaaagatc tgaccaaaat cgttcgtggt gaagcagaac aagcgcgtgt
    34741 tgcagtacgt aatgtgcgtc gtgacgcgaa cgacaaagtg aaagcactgt tgaaagataa
    34801 agagatcagc gaagacgacg atcgccgttc tcaggacgat gtacagaaac tgactgatgc
    34861 tgcaatcaag aaaattgaag cggcgctggc agacaaagaa gcagaactga tgcagttctg
    34921 atttcttgaa cgacaaaaac gccgctcagt agatccttgc ggatcggctg gcggcgtttt
    34981 gctttttatt ctgtctcaac tctggatgtt tcatgaagca actcaccatt ctgggctcga
    35041 ccggctcgat tggttgcagc acgctggacg tggtgcgcca taatcccgaa cacttccgcg
    35101 tagttgcgct ggtggcaggc aaaaatgtca ctcgcatggt agaacagtgc ctggaattct
    35161 ctccccgcta tgccgtaatg gacgatgaag cgagtgcgaa acttcttaaa acgatgctac
    35221 agcaacaggg gagccgcacc gaagtcttaa gtgggcaaca agccgcttgc gatatggctg
    35281 cacttgagga tgttgatcag gtgatggcag ccattgttgg cgctgctggg ctgttaccta
    35341 cgcttgctgc gatccgcgcg ggtaaaacca ttttgctggc caataaagaa tcactggtta
    35401 cctgcggacg tctgtttatg gacgccgtaa agcagagcaa agcgcaattg ttaccggtcg
    35461 atagcgaaca taacgctatt tttcagagtt taccgcaacc tatccagcat aatctgggat
    35521 acgctgacct tgagcaaaat ggcgtggtgt ccattttact taccgggtct ggtggccctt
    35581 tccgtgagac gccattgcgc gatttggcaa caatgacgcc cgatcaagcc tgccgtcatc
    35641 cgaactggtc gatgggacgt aaaatttccg tcgattcggc taccatgatg aacaaaggtc
    35701 tggaatacat tgaagcgcgt tggctgttta acgccagcgc cagccagatg gaagtgctga
    35761 ttcacccgca gtcagtgatt cactcaatgg tgcgctacca ggacggcagt gttctggcgc
    35821 agctgggtga accggatatg cgtacgccaa ttgctcacac catggcatgg ccgaatcgcg
    35881 tgaactctgg cgtgaagccg ctcgattttt gcaaactaag tgcgttgaca tttgccgcac
    35941 cggattatga tcgttatcca tgccttaaac tggcgatgga ggcgttcgaa caaggccagg
    36001 cagcgacgac agcattgaat gccgcaaacg aaatcaccgt ggctgctttt cttgcgcaac
    36061 aaatccgctt tacggatatc gctgcgttga atttatccgt actggaaaaa atggatatgc
    36121 gcgaaccaca atgtgtggat gatgtgttat ctgttgatgc gaacgcgcgt gaagtcgcca
    36181 gaaaagaggt gatgcgtctc gcaagctgag gataatccgg ctacagagag tcgcgctatt
    36241 tgttagcgta gggcttcagt gatatagtct gcgccatctg atcgtaagta gttggcttta
    36301 taaggtcagg tatgccgtgg ttttacacgg cttttttttg tataggcttc agtattcctg
    36361 agtaccgtaa accctgtcag ggaataagaa acgcgtgatg ttgtctgcta ctcaaccact
    36421 tagcgaaaaa ttgccagcgc atggctgtcg tcatgttgcg atcattatgg acggcaatgg
    36481 tcgctgggca aaaaagcaag ggaagattcg tgcctttggg cataaagccg gggcaaaatc
    36541 cgtccgccgg gctgtctctt ttgcggccaa caacggtatt gaggcgttaa cgctgtatgc
    36601 ctttagtagt gaaaactgga accgaccagc gcaggaagtc agtgcgttaa tggaactgtt
    36661 tgtgtgggcg ctagatagcg aagtaaaaag tctgcaccga cataacgtgc gtctgcgtat
    36721 tattggcgat accagtcgct ttaactcgcg tttgcaagaa cgtattcgta aatctgaagc
    36781 gctaacagcc gggaataccg gtctgacgct gaatattgcg gcgaactacg gtggacgttg
    36841 ggatatagtc cagggagtca ggcaactggc tgaaaaggtg cagcaaggaa acctgcaacc
    36901 agatcagata gatgaagaga tgctaaacca gcatgtctgt atgcatgaac tggcccctgt
    36961 agatttagta attaggactg ggggggagca tcgcattagt aactttttgc tttggcaaat
    37021 tgcctatgcc gaactttact ttacagatgt tctctggccc gatttcgatg aacaagactt
    37081 tgaaggggcg ttaaatgcct ttgctaatcg agagcgtcgt ttcggcggca ccgagcccgg
    37141 tgatgaaaca gcctgatggg ggtcgctttt gctgaagtat cgcctgatat ctgcttttgt
    37201 gttaataccc gtcgtcatcg cggcgttgtt tctgttgccg ccggtggggt tcgccattgt
    37261 aacgctggtg gtctgtatgc tggcagcgtg ggaatgggga cagcttagcg gttttaccac
    37321 tcgttcgcag cgagtatggt tggcggtgtt atgtgggtta ttgttggcgc tgatgctttt
    37381 tctgttgccg gaatatcacc gaaatattca tcaaccgctg gttgaaatct cactttgggc
    37441 ttcgctgggt tggtggattg tcgcgctatt gctggtgctg ttttacccag gttccgcagc
    37501 aatctggcgt aactctaaaa cattgcgcat tatttttggc gtgctaacca ttgttccctt
    37561 cttctggggc atgctggcgt tacgggcctg gcactacgac gagaatcatt acagtggcgc
    37621 aatatggctg ctctatgtca tgatcctggt atggggcgct gactctggcg catatatgtt
    37681 tggcaaattg tttggtaaac ataaactggc accgaaggtt tctccgggta aaacctggca
    37741 aggctttatc ggtggactag tgaccgcagc ggtaatctca tggggttatg gcatgtgggt
    37801 gaatctcgac gtcgctcccg tcaccttact catttgctct attgtcgcag cgttagcctc
    37861 agtgctcggc gatctgaccg aaagtatgtt taagcgtgaa gcaggaatta aggacagcgg
    37921 tcatttaatt ccaggacacg gtggtatttt agatcgtatt gatagcctga cggctgcggt
    37981 accggtcttt gcttgcttgt tgttactggt attcaggacg ctttaacgga aggtaatatg
    38041 ctgagttttc tctgggattt ggcttcgttc atcgttgcac tgggtgtact tatcaccgtg
    38101 catgaatttg gtcatttctg ggttgcccgg cgttgtggtg ttcgcgttga gcgtttctca
    38161 atagggtttg gtaaggcgct ctggcggcga actgataagc tcggcaccga atatgttatc
    38221 gccctgatcc cgttgggcgg ttatgtcaaa atgctggatg agcgcgcaga accggtcgtt
    38281 ccggaactcc gccaccatgc cttcaataat aaatctgtcg gccaacgagc ggcgattatt
    38341 gccgcaggtc cggttgcaaa cttcattttt gctatctttg cctactggct ggtttttatt
    38401 attggtgtgc ctggcgtacg tccggtggtt ggtgaaatag cagccaattc gatagctgcg
    38461 gaagcacaaa ttgcaccagg tacggaacta aaagccgtag atggtatcga aacgcctgat
    38521 tgggatgccg tgcgtttgca gttggtcgat aaaattggcg atgaaagcac caccattacg
    38581 atagcgccat ttggcagcga ccaacggcgg gatgtaaagc tcgatttacg tcactgggcg
    38641 tttgagcctg ataaagaaga tccggtatct tcgttgggga ttcgtcctcg tgggccgcaa
    38701 attgaacctg tactggaaaa tgtgcagcca aactcggcgg caagcaaggc aggtttgcaa
    38761 gcaggcgaca ggatcgttaa agtcgatggt cagcccttaa cgcagtgggt gacctttgtg
    38821 atgcttgtcc gggataaccc gggtaaatcc ttagcgttag aaatcgaaag acaggggagt
    38881 cccttgtctt tgacattaat cccggagagt aaaccgggta atggtaaagc gattggtttt
    38941 gtcggtattg agccgaaagt cattcctttg cctgatgagt ataaagttgt acgccagtat
    39001 gggccgttca acgctatcgt cgaagccacg gacaaaacgt ggcagttgat gaagctgacg
    39061 gtcagtatgc tgggaaaatt gatcaccggt gatgtgaaac tgaacaacct cagtgggccg
    39121 atctctatcg ccaagggggc tgggatgaca gcggaactcg gggtagttta ttacctgccg
    39181 tttcttgcgc ttattagcgt gaacttaggg ataattaacc tgtttccgtt gcccgtactt
    39241 gacggggggc atctgctgtt ccttgcgatc gaaaagatca agggcggacc ggtatccgag
    39301 cgggttcaag acttttgtta tcgcattggc tcgattctgc tggtgctgtt aatggggctt
    39361 gcacttttca atgatttctc tcggttatga gagttagtta ggaagaacgc ataataacga
    39421 tggcgatgaa aaagttgctc atagcgtcgc tgctgtttag cagcgccacc gtatacggtg
    39481 ctgaagggtt cgtagtgaaa gatattcatt tcgaaggcct tcagcgtgtc gccgttggtg
    39541 cggccctcct cagtatgccg gtgcgcacag gcgacacggt taatgatgaa gatatcagta
    39601 ataccattcg cgctctgttt gctaccggca actttgagga tgttcgcgtc cttcgtgatg
    39661 gtgataccct tctggttcag gtaaaagaac gtccgaccat tgccagcatt actttctccg
    39721 gtaacaaatc ggtgaaagat gacatgctga agcaaaacct cgaggcttct ggtgtgcgtg
    39781 tgggcgaatc cctcgatcgc accaccattg ccgatatcga gaaaggtctg gaagacttct
    39841 actacagcgt cggtaaatat agcgccagcg ttaaagctgt cgtgaccccg ctgccgcgca
    39901 accgtgttga cctaaaactg gtgttccagg aaggtgtgtc agctgaaatc cagcaaatta
    39961 acattgttgg taaccatgct ttcaccaccg atgaactgat ctctcatttc caactgcgtg
    40021 acgaagtgcc gtggtggaac gtggtaggcg atcgtaaata ccagaaacag aaacttgcgg
    40081 gcgaccttga aaccctgcgc agttactatc tggatcgcgg ttatgcccgt ttcaacatcg
    40141 attctaccca ggtcagtctg acgccagata aaaaaggtat ttacgtcacg gtgaacatca
    40201 ccgaaggcga tcagtacaag ctttctggcg ttgaagttag cggcaatctt gccgggcact
    40261 ccgctgaaat tgagcaactg actaagatcg agccgggcga gctgtataac ggcaccaaag
    40321 tgaccaaaat ggaagatgac attaaaaagc ttcttggtcg ctatggttat gcctatccgc
    40381 gcgtacagtc gatgcctgaa attaacgatg ccgacaaaac cgttaaatta cgcgtgaacg
    40441 ttgatgcggg taaccgtttc tatgtgcgta agatccgttt tgaaggtaac gatacctcga
    40501 aagatgccgt cctgcgtcgc gaaatgcgtc agatggaagg tgcatggctg gggagcgatc
    40561 tggtcgatca gggtaaggag cgtctgaacc gtctgggctt ctttgaaact gtcgataccg
    40621 atacccaacg tgttccgggg agcccggatc aggttgatgt agtttacaag gtgaaagaac
    40681 gtaacaccgg tagcttcaac ttcgggattg gttacggtac tgaaagtggc gtgagcttcc
    40741 aggctggtgt gcaacaggat aactggttag gtacgggtta tgctgttggt atcaacggga
    40801 ccaaaaacga ttaccagacc tatgctgaac tgtcggtaac caacccgtac ttcaccgtag
    40861 atggtgtaag cctcggtggt cgtctcttct ataatgactt ccaggcagat gacgccgacc
    40921 tgtccgacta taccaacaag agttatggta cagacgtgac gttgggcttc ccgatcaacg
    40981 aatataactc gctgcgtgca ggtctgggtt atgtacataa ctccctgtcc aacatgcagc
    41041 ctcaggttgc gatgtggcgt tatctgtact ctatgggtga acatccgagc acctctgatc
    41101 aggataacag cttcaaaacg gacgacttca cgttcaacta tggttggacc tataacaagc
    41161 ttgaccgtgg ttacttcccg acagatggtt cacgtgtcaa cctgaccggt aaagtgacca
    41221 ttccgggatc ggataacgaa tactacaaag tgacgttaga cacggcgact tatgtgccga
    41281 ttgatgacga tcacaaatgg gttgttctgg gtcgtacccg ctggggttat ggtgatggtt
    41341 taggcggcaa agagatgccg ttctatgaga acttctatgc cggtggttcc agcaccgtgc
    41401 gtggcttcca gtccaatacc attggtccga aagcagttta cttcccgcat caggccagta
    41461 attatgatcc ggactatgat tacgaatgtg cgactcagga cggcgcgaaa gatctgtgta
    41521 aatcggatga tgctgtaggc ggtaacgcca tggcggttgc cagcctcgag ttcatcaccc
    41581 cgacgccgtt tattagcgat aagtatgcta actcggttcg tacttccttc ttctgggata
    41641 tgggtaccgt ttgggataca aactgggatt ccagccaata ttctggatat ccggactata
    41701 gtgatccaag caatatccgt atgtctgcgg gtatcgcatt acaatggatg tccccattgg
    41761 ggccgttggt gttctcctac gcccagccgt tcaaaaagta cgatggagac aaggcagaac
    41821 agttccagtt taacatcggt aaaacctggt aagtgttctc cacaaaggaa tgtagtggta
    41881 gtgtagcgat gactttaggc gatcaatata agatcgccgg gccacgcaaa gaactgcacc
    41941 ctccggtgca aatgggatgg taaggagttt attgtgaaaa agtggttatt agctgcaggt
    42001 ctcggtttag cactggcaac ttctgctcag gcggctgaca aaattgcaat cgtcaatatg
    42061 ggcagcctgt tccagcaggt agcgcagaaa accggtgttt ctaacacgct ggaaaacgag
    42121 ttcaaaggcc gtgccagcga actgcagcgt atggaaaccg atctgcaggc taaaatgaaa
    42181 aagctgcagt ccatgaaagc gggcagcgat cgcactaagc tggaaaaaga cgtgatggct
    42241 cagcgccaga cttttgctca gaaagcgcag gcttttgagc aggatcgcgc acgtcgttcc
    42301 aacgaagaac gcggcaaact ggttactcgt atccagactg ctgtgaaatc cgttgccaac
    42361 agccaggata tcgatctggt tgttgatgca aacgccgttg cttacaacag cagcgatgta
    42421 aaagacatca ctgccgacgt actgaaacag gttaaataag taatgccttc aattcgactg
    42481 gctgatttag cgcagcagtt ggatgcagaa ctacacggtg atggcgatat cgtcatcacc
    42541 ggcgttgcgt ccatgcaatc tgcacaaaca ggtcacatta cgttcatggt taatccaaaa
    42601 taccgtgagc atttaggctt gtgccaggcg tccgcggttg tcatgaccca ggacgatctt
    42661 cctttcgcga aaagtgccgc gctggtagtg aagaatccct acctgactta cgcgcgcatg
    42721 gcgcaaattt tagataccac gccgcagccc gcgcagaaca ttgcacccag tgcggtgatt
    42781 gacgcgacgg cgaagctggg taacaacgta tcgattggcg ctaacgcggt gattgagtcc
    42841 ggcgttgaac tgggcgataa cgtgattatc ggtgccggtt gcttcgtagg taaaaacagc
    42901 aaaatcggtg caggttcgcg tctctgggcg aacgtaacca tttaccatga gatccagatc
    42961 ggtcagaatt gcctgatcca gtccggaaca gtggtaggcg cagacggctt tggttatgcc
    43021 aacgatcgtg gtaactgggt gaagatccca cagattggtc gggtaattat tggcgatcgc
    43081 gtggagatcg gtgcctgcac tactattgat cgtggcgcgc tggatgacac tgttattggc
    43141 aatggtgtga tcattgataa ccagtgccag attgcacata acgtcgtgat tggcgacaat
    43201 acggcagttg ccggtggcgt cattatggcg ggcagcctga aaattggtcg ttactgcatg
    43261 atcggcggag ccagcgtaat caacgggcat atggaaatat gcgacaaagt gacggttacg
    43321 ggcatgggta tggtgatgcg tcccatcact gaaccaggcg tctattcctc aggcattccg
    43381 ctgcaaccca acaaagtctg gcgcaaaacc gctgcactgg tgatgaacat tgatgacatg
    43441 agcaagcgtc tgaaatcgct tgagcgcaag gttaatcaac aagactaacg ttccatcttt
    43501 tgttcgccaa actttacggc ctgtctcatt cttacgattg cggcaggccg tgttattatt
    43561 gtcgtttctt atattttgac aggaagagta tcttgactac taacactcat actctgcaga
    43621 ttgaagagat tttagaactt ctgccgcacc gtttcccgtt cttactggtg gatcgcgtgc
    43681 tggattttga agaaggtcgt tttctgcgcg cagtaaaaaa tgtctctgtc aatgagccat
    43741 tcttccaggg ccatttccct ggaaaaccga ttttcccggg tgtgctgatt ctggaagcaa
    43801 tggcacaggc aacaggtatt ctggcgttta aaagcgtagg aaaactggaa ccgggtgagc
    43861 tgtactactt cgccggtatt gacgaagcgc gctttaagcg cccggtcgtg cctggcgatc
    43921 aaatgatcat ggaagtcact ttcgaaaaaa cgcgccgcgg cctgacccgt tttaaagggg
    43981 ttgctctggt cgacggtaaa gtagtttgcg aagcaacgat gatgtgtgct cgtagccggg
    44041 aggcctgata cgtgattgat aaatccgcct ttgtgcatcc aaccgccatt gtggaagagg
    44101 gcgcgtcgat tggcgcgaac gctcacattg gtcctttttg tatcgttgga ccccatgtcg
    44161 aaattggtga gggtaccgta ctgaaatctc acgttgtcgt gaatggtcat actaaaattg
    44221 gccgcgataa tgagatttat cagttcgcct ccatcggcga agttaaccag gatctgaaat
    44281 atgctggcga accgacccgc gtggaaatcg gcgatcgtaa ccgcattcgc gaaagcgtca
    44341 ccattcatcg tggcacagtc cagggcggtg gattgacgaa ggtgggcagc gacaacttac
    44401 tgatgatcaa tgcgcacatt gcgcacgatt gtacggtagg taaccgctgc atcctcgcca
    44461 acaacgcaac gctggcgggt cacgtatcgg ttgacgactt cgcgatcatc ggcggcatga
    44521 ccgcagtcca tcagttctgc atcattggtg cgcatgtgat ggttggcggc tgctccggtg
    44581 tggcgcagga cgtccctcct tatgtcattg cgcagggtaa ccacgcaacg ccgttcggtg
    44641 tcaatatcga agggctgaag cgccgcggat tcagccgtga ggcgattacc gctatccgca
    44701 atgcgtataa gctgatttat cgtagcggta aaacgctcga tgaagtgaaa ccggaaattg
    44761 ctgaactggc ggaaacatat ccggaagtga aagcctttac cgatttcttt gcacgctcaa
    44821 cgcgcggtct gattcgttaa tgactgaaca gcgtccatta acgattgccc tggtcgccgg
    44881 agaaacctcc ggcgatatcc tgggggccgg tttaatccgc gctctgaaag aacgtgtgcc
    44941 caacgcccgc tttgttggtg ttgccgggcc acgaatgcag gctgaaggct gcgaagcctg
    45001 gtacgaaatg gaagaactgg cagtgatggg cattgttgaa gtgctcggtc gtctgcgtcg
    45061 cttactacat attcgtgccg atctgacaaa gcgttttggc gaactgaagc cagatgtttt
    45121 tgttggtatt gatgcgcctg acttcaatat tactcttgaa ggtaacctca aaaagcaggg
    45181 tatcaaaacc attcattacg tcagcccgtc cgtctgggcg tggcgacaga aacgcgtttt
    45241 caaaataggc agagccaccg atctggtgct cgcatttctg cctttcgaaa aagcgtttta
    45301 tgacaaatac aacgtaccgt gccgctttat cggtcatacc atggctgatg ccatgccatt
    45361 agatccagat aaaaatggtg cccgtgatgt gctggggatc ccttacgatg cccactgtct
    45421 ggcattgttg ccgggcagcc gtggtgcaga agtcgaaatg cttagtgccg atttcctgaa
    45481 aacggcccag cttttgcgcc agacatatcc ggatcttgaa atcgtggtgc cgctggtgaa
    45541 tgccaaacgc cgcgagcagt ttgaacgcat caaagctgca gtcgcgccag acctgtcggt
    45601 tcatttgctg gatggaatgg gccgtgaggc gatggtcgcc agtgatgcgg cgctactggc
    45661 gtcgggtacg gcagcactgg agtgtatgct ggctaaatgc ccgatggtag tgggatatcg
    45721 catgaagcct ttcaccttct ggctggcgaa gcgactggtg aaaactgatt atgtctcgct
    45781 gccaaatctg ctggcgggca gagagttagt caaagagcta ttgcaggaag agtgtgagcc
    45841 gcaaaaattg gctgcggcgc tgttaccgct gttggcgaac gggaaaacca gccatgcgat
    45901 gcacgacacc ttccgcgagc tgcatcagca gatccgctgc aatgccgatg agcaggcggc
    45961 acaagccgtt ctggagttag cacaatgatc gaatttgtct atccgcacac gcagctggtt
    46021 gcgggtgtgg atgaagtcgg acgcgggccg ttagttggcg cggtcgtcac cgctgcggtg
    46081 atccttgacc cggcgcgccc aattgccggg ctgaatgatt ccaaaaagct gagcgaaaaa
    46141 cgccgtctgg cgctctgtga agagatcaaa gagaaagcgt tgagctggag tctgggccgc
    46201 gcggaacctc acgaaatcga cgaactgaat attttacatg cgaccatgct ggcgatgcag
    46261 cgtgccgtcg ctggactgca tattgcgccg gaatatgtgt tgattgatgg taaccgctgc
    46321 ccgaaattac agatgccttc gatggctgtg gtgaaaggcg atagccgcgt accggaaatc
    46381 agtgccgcgt ctatcttggc gaaagtgacg cgtgacgccg aaatggcagc gctggatatt
    46441 gttttcccgc aatatggttt tgcccaacac aaagggtatc caaccgcttt tcatctggaa
    46501 aaactggctg aacacggcgc gaccgaacac catcggcgca gctttgggcc tgtcaaacgc
    46561 gcactgggac ttgcgtcctg attcttgtgt cgagattaag taaaccggaa tctgaagatg
    46621 tctgaaccac gtttcgtaca cctgcgggtg cacagcgact actcgatgat cgatggcctg
    46681 gccaaaaccg caccgctggt aaaaaaggcg gcagcgttgg gtatgccagc actggcgatc
    46741 accgatttca ccaacctttg cggtctggtg aagttctacg gagcgggaca tggcgcaggg
    46801 attaagccca tcgtcggggc ggattttaac gtccagtgcg acctgctggg tgatgagtta
    46861 actcacctga cggtgctggc ggcgaacaat accggctatc agaatctgac gttgctgatc
    46921 tcaaaagcgt atcagcgcgg gtacggtacc gccgggccga tcatcgatcg cgactggctt
    46981 atcgaattaa atgaagggtt gatccttctt tccggcgggc gcatgggcga cgttggacgc
    47041 agtcttttgc gtggtaacag cgcgctggta gatgagtgtg tcgcgtttta tgaagaacac
    47101 ttcccggatc gctattttct cgagctgatc cgcaccggca ggccggacga agaaagctat
    47161 ctgcacgctg cggtggaact ggcggaagca cgcggtttgc ccgttgtggc gaccaacgac
    47221 gtgcgcttta tcgacagcag cgactttgac gcacacgaaa tccgcgtcgc gatccacgac
    47281 ggctttaccc tcgacgatcc taaacgcccg cgtaactatt cgccgcagca atatatgcgt
    47341 agcgaagagg agatgtgcga gctttttgcc gacattcccg aagcccttgc caacaccgta
    47401 gagatagcca aacgttgtaa cgtaactgtg cgtctgggtg aatacttctt gccgcaattc
    47461 ccgaccgggg acatgagtac cgaagattat ctggtcaagc gtgcaaaaga aggcctggaa
    47521 gagcgtctgg catttttatt ccctgacgag gaagaacgtc ttaagcgtcg tccggagtat
    47581 gacgaacgtc tggatactga acttcaggtt atcaaccaga tgggcttccc gggttacttc
    47641 ctcatcgtta tggaatttat ccagtggtcg aaagataacg gtgtaccggt agggccaggc
    47701 cgtggttctg gtgctgggtc actggtggcc tacgcgttaa aaatcaccga tctcgatccg
    47761 ctggaatttg acctgctgtt cgaacgtttc cttaacccgg aacgtgtctc catgcctgac
    47821 ttcgacgttg acttctgtat ggagaaacgc gatcaggtta tcgagcacgt agcggacatg
    47881 tacggtcgtg atgcggtatc gcagattatc accttcggta cgatggcggc gaaagcggtg
    47941 atccgcgacg taggccgcgt gctggggcat ccgtacggct ttgtcgatcg tatctcgaaa
    48001 ctgatcccgc ccgatccggg tatgacgctg gcgaaagcgt ttgaagccga gccacagctg
    48061 ccggaaatct acgaagcgga tgaagaagtt aaggcgctga tcgacatggc gcgcaaactg
    48121 gaaggggtca cccgtaacgc cggtaagcac gccggtgggg tggttatcgc accgaccaaa
    48181 attaccgatt ttgcgccgct ttactgcgat gaagagggca aacatccggt cacccagttt
    48241 gataaaagcg acgttgaata cgccgggctg gtgaagttcg acttcctcgg tttgcgtacg
    48301 ctcaccatca tcaactgggc gctggagatg atcaacaagc ggcgggcgaa gaatggcgag
    48361 ccgccgctgg atatcgccgc gatcccgctg gatgacaaga aaagcttcga catgctgcaa
    48421 cgctcggaaa ccacggcggt attccagctt gaatcgcgcg gcatgaagga cctgatcaag
    48481 cgtctgcaac ctgactgctt cgaagatatg atcgcactgg tggcactgtt ccgccccggc
    48541 ccgttgcaat cagggatggt ggataacttt atcgaccgta aacatggtcg cgaagagatc
    48601 tcctatccgg acgtgcagtg gcagcatgaa agcctgaaac cggtactgga gccaacctac
    48661 ggcatcatcc tgtatcagga acaggtcatg cagattgccc aggtgctttc tggttatacc
    48721 ctcggtggcg cggatatgct gcgtcgtgcg atgggtaaga aaaagccgga agagatggct
    48781 aagcagcgtt ctgtatttgc tgaaggtgca gaaaagaacg gaatcaacgc cgaactggcg
    48841 atgaaaatct tcgacctggt ggagaaattc gcgggttacg gatttaacaa atcgcactcg
    48901 gcggcctatg ctttggtgtc atatcaaacg ttatggctga aagcgcacta tccggcggag
    48961 tttatggcgg cggtaatgac cgccgatatg gacaacaccg agaaggtggt gggcctggtg
    49021 gatgagtgct ggcggatggg gctgaaaatc ctgccaccag atataaactc cggtctttac
    49081 catttccacg tcaacgacga cggcgaaatc gtgtatggta ttggcgcgat caaaggggta
    49141 ggtgaaggcc cgattgaggc catcatcgaa gcccgtaata aaggcggcta cttccgcgaa
    49201 ctgtttgatc tctgcgcccg taccgacacc aaaaagttaa accggcgggt gctggaaaaa
    49261 ctgatcatgt ccggggcgtt tgaccgtctt gggccacacc gcgcggcgct gatgaactcg
    49321 ctgggcgatg cgttaaaagc ggcagatcaa cacgcgaaag cggaagctat cggtcaggcc
    49381 gatatgttcg gcgtgctggc cgaagagccg gaacaaattg aacaatccta cgccagctgc
    49441 caaccgtggc cggagcaggt ggtattagat ggggaacgtg aaacgttagg tctgtacctg
    49501 acgggacacc ctatcaacca gtatttaaaa gagattgagc gttatgtcgg aggcgtaagg
    49561 ctgaaagaca tgcacccgac agaacgtggt aaagtcatca cggctgcggg gctcgttgtt
    49621 gccgcgcggg ttatggtcac caagcgcggc aatcgtatcg gtatctgcac gctggatgac
    49681 cgttccgggc ggctggaagt gatgttgttt actgacgccc tggataaata ccagcaattg
    49741 ctggaaaaag accgcatact tatcgtcagc ggacaggtca gctttgatga cttcagcggt
    49801 gggcttaaaa tgaccgctcg cgaagtgatg gatattgacg aagcccggga aaaatatgct
    49861 cgcgggcttg ctatctcgct gacggacagg caaattgatg accagctttt aaaccgactc
    49921 cgtcagtctc tggaacccca ccgctctggg acaattccag tacatctcta ctatcagagg
    49981 gcggatgcac gcgcgcgatt gcgttttggc gcgacgtggc gtgtctctcc gagcgatcgt
    50041 ttattaaacg atctccgtgg cctcattggt tcggagcagg tggaactgga gtttgactaa
    50101 tacaggaata ctatgagtct gaatttcctt gattttgaac agccgattgc agagctggaa
    50161 gcgaaaatcg attctctgac cgcggttagc cgtcaggatg agaaactgga tattaacatc
    50221 gatgaagaag tgcatcgtct gcgtgaaaaa agcgtagaac tgacgcgtaa aatcttcgcc
    50281 gatctcggtg catggcagat tgcgcaactg gcacgccatc cacagcgtcc ttataccctg
    50341 gattacgttc gcctggcatt tgatgaattt gacgaactgg ctggcgaccg cgcgtatgca
    50401 gacgataaag ctatcgtcgg tggtatcgcc cgtctcgatg gtcgtccggt gatgatcatt
    50461 ggtcatcaaa aaggtcgtga aaccaaagaa aaaattcgcc gtaactttgg tatgccagcg
    50521 ccagaaggtt accgcaaagc actgcgtctg atgcaaatgg ctgaacgttt taagatgcct
    50581 atcatcacct ttatcgacac cccgggggct tatccgggcg tgggcgcaga agaacgtggt
    50641 cagtcggaag ccattgcacg caacctgcgt gaaatgtctc gcctcggcgt accggtagtt
    50701 tgtacggtta tcggtgaagg tggttctggt ggtgcgctgg cgattggcgt gggcgataaa
    50761 gtgaatatgc tgcaatacag cacctattcc gttatctcgc cggaaggttg tgcgtccatt
    50821 ctgtggaaga gcgctgacaa agcgccgctg gcggctgaag cgatgggtat catcgctcca
    50881 cgtctgaaag agctgaaact gatcgactcc atcatcccgg aaccgctggg cggtgctcac
    50941 cgtaacccgg aagcgatggc ggcgtctctg aaagcgcagc tgctggcgga tctggccgat
    51001 ctcgacgtgt taagcactga agatttaaaa aatcgtcgtt atcagcgcct gatgagctac
    51061 ggctacgcgt aatcggcaaa tgttctgaaa agggtcactt cggtggccct tttttatcgc
    51121 cacggtttga gcaggctatg attaaggaag gattttccag gaggaacaca tgaacatcat
    51181 tgccattatg ggaccgcatg gcgtctttta taaagatgag cccatcaaag aactggagtc
    51241 ggcgctggtg gcgcaaggct ttcagattat ctggccacaa aacagcgttg atttgctgaa
    51301 gtttatcgaa cataacccac gaatttgcgg cgtgattttt gactgggatg agtacagtct
    51361 cgatttatgt agcgatatca atcaacttaa tgaatatctc ccgctttatg ccttcatcaa
    51421 cacccactcg acgatggatg tcagcgtgca ggatatgcgg atggcgctct ggttttttga
    51481 atatgcgctg gggcaggcgg aagatatcgc cattcgtatg cgtcagtaca ccaacgaata
    51541 tcttgataac attacgccgc cgttcacgaa agccttgttt acctacgtca aagagcggaa
    51601 gtacaccttt tgtacgccgg ggcatatggg cggcaccgca tatcaaaaaa gcccggttgg
    51661 ctgtctgttt tatgattttt tcggcgggaa taccctcaag gcggacgtct ctatttcggt
    51721 caccgagctt ggttcgttgc tcgaccacac cgggccacac ctggaagcgg aagagtacat
    51781 cgcgcggacg tttggcgcgg aacagagtta tatcgttacc aacggaacat cgacgtcgaa
    51841 caaaattgtg ggtatgtacg ccgcgccatc cggcagtacg ctgttgatcg accgcaattg
    51901 tcataaatcg ctggcgcatc tgttgatgat gaacgatgta gtgccagtct ggctgaaacc
    51961 gacgcgtaat gcgttgggga ttctgggtgg gatcccgcgc ggtgaattta ctcgcgacag
    52021 catcgaagag aaagtcgctg ccaccacgca ggcacaatgg ccggttcatg cggtgatcac
    52081 caactccacc tatgatggct tgctctacaa caccgactgg atcaaacaga cgctggatgt
    52141 cccgtcgatt cacttcgatt ctgcctgggt gccgtacacc cattttcatc cgatctacca
    52201 gggtaaaagt ggtatgagcg gcgagcgtgt tgcgggaaaa gtgatcttcg aaacgcaatc
    52261 tacccacaaa atgctggcgg cgttatcgca ggcttcgctg atccacatta aaggcgagta
    52321 tgacgaggag gcgtttaacg aagcctttat gatgcatacc accacctcgc ccagttatcc
    52381 cattgttgct tcggttgaga cggcggcggc gatgctgcgt ggtaatcctg gcaaacggct
    52441 gattaaccgt tcagtagaac gagctctgca ttttcgcaaa gaggtccagc ggctgcggga
    52501 agagtctgac ggctggtttt ttgatatctg gcaaccgccg caggtggatg aagccgaatg
    52561 ctggcccgtt gcgcctggcg aacagtggca cggctttagc gatgcggatg ccaatcacat
    52621 gtttctcgat ccggttaaag tcactatttt gacaccgggg atggacgagc agggcaatat
    52681 gagcgaggag gggatcccgg cggcgctggt ggcaaaattc ctcgacgaac gtgggatcgt
    52741 agtagagaaa accggccctt ataacctgct gtttctcttt agtattggca tcgataaaac
    52801 caaagcaatg ggattattgc gtgggttgac ggaatttaag cgctcttacg atctcaacct
    52861 gaggatcaaa aatatgctac ccgacctcta tgcagaagat cccgatttct accgcaatat
    52921 gcgtattcag gatctggcgc aagggatcca taagctgatt cgtaaacacg atcttcccgg
    52981 tttgatgttg cgggcatttg ataccttgcc ggagatgatc atgacgccac atcaggcatg
    53041 gcagagacag attaaaggcg aagtagaaac cattgcgctg gaacaactgg tcggtagggt
    53101 gtcggcaaat atgatcctgc cttatccacc cggcgtaccg ctgctgatgc cgggagaaat
    53161 gcttaccgaa gagagccgca cggtactcga ttttctactg atgctttgtt ccgtagggca
    53221 acattacccc ggttttgaaa cggatattca cggcgcgaaa caggacgaag acggtgttta
    53281 ccgcgtacga gtcctaaaaa tggcaggata acttgccaga gcggcttccg ggcgagtaac
    53341 gtgctgttaa caaataaagg agacgttatg ctgggtttaa aacaggttca ccatattgcg
    53401 attattgcga cggattatgc gatgagcaaa gctttctact gcgatattct tggtttcacg
    53461 ctgcaaagcg aagtctatcg cgaagcgcgc gactcgtgga aaggggattt ggcgcttaat
    53521 gggcaatatg tgattgagct tttctcattt ccgttcccgc cggagcgccc cagccgaccg
    53581 gaagcttgcg gcctgcgtca tctggctttt agcgttgatg atatcgatgc ggcagtggcg
    53641 caccttgaaa gccataacgt gaagtgtgaa gccatccgtg tcgatcccta cacgcaaaaa
    53701 cgcttcacgt tctttaacga tccggacggg ctgccgttgg aactgtatga gcagtaaggc
    53761 ttgtcatcgc cgcatttgcc cggtaacgtg cccggcattg ctactgtaaa atcgcaccat
    53821 catgacactc acgctcaata gacaacttct ctcctcacgc cagattctgg tggcctttag
    53881 cggcggcctt gattctactg tcttactgca tcaactggtg cagtggcgga cggaaaatcc
    53941 gggcgtcact ctgcgcgcta tccatgtgca tcacggttta agcgccaatg ctgatgcctg
    54001 ggtgaaacat tgtgaaaaca tctgccaaca gtggcaggtg ccgctggtgg tcgaacgtgt
    54061 gcagcttgcg caagaggggc taggtattga ggcccaggcg cggcaggcac gttatcaggc
    54121 atttgcccgc accttgttgc ccggtgaagt gctggttaca gcgcaacatc tcgacgatca
    54181 atgtgaaacc tttctgctgg cgctaaaacg cggtagcggc cctgccgggc tttcggctat
    54241 ggcggaagtt tcggagtttg ccggaacaca gcttatccgc ccgttgcttg cccgcacgcg
    54301 gggggaactg gtgcagtggg cgttggcgca tggtttacgc tggattgaag acgaaagtaa
    54361 tcaggatgat agctacgatc gtaactttct gcgcctgcgc gtagtgccgt tattgcagca
    54421 gcgttggccg cattttgccg aagcaacagc ccgcagcgcc gcactttgcg ctgaacaaga
    54481 gagcctgtta gatgaactgc tggcggatga tttagcgcac tgtcaaactc cgcaggggac
    54541 gctgcagatt gcgccaatgc tggcgatgag tgatgcccgt cgcgcggcga ttatccgccg
    54601 ctggctggca gggcagaatg caccgatgcc ttcccgcgac gcgttggtga ggatctggca
    54661 ggaagtggcg ctggcgcgag aagatgcttc cccctgttta cgtttgggtg cgtttgaaat
    54721 ccgccgctat cagtcgcaac tgtggtggat taaatccgtc actgggcaaa gcgaaaccat
    54781 tctgccgtgg cagacgtggc tacaaccgct ggaattaccg gcggggctgg gaagtgtaca
    54841 gcttactgcg ggaggcgata ttcgccctcc gcgtgcagac gaagcggtca gcgtgcgttt
    54901 caaagcgcca ggattgctgc atattgtcgg gcgcaacggc ggacgtaagc tgaagaaaat
    54961 ctggcaagag ttaggcgttc cgccgtggct acgtgacacc acgccactgc tgttttatgg
    55021 cgaaacgctg attgcggcgg caggggtatt tgtgacgcaa gaaggtgtgg ctgtaggtga
    55081 gaatggcgtc agttttgtct ggcagaaaac gcttagtcaa gtgaaagccg gataagacgc
    55141 atcaaacgtc gcatccggcg aaagtcaatc aggactcgct caccaccacc gtaccgattt
    55201 ccgggtggct aaagctggta attttatcca gacgcagctc gcgggttgca ccagcggcct
    55261 cgacgaccag atactccaca tttttgcggg agactaaatc gctggccttc gcctgcaatt
    55321 tttcgccatc tttcagctca agtgccagca ttaaatgatg ctggcaggcg agctcaagat
    55381 tatcgtaatc atcacaattg attggttgat acgtatcatt cattgacata atcgctcacc
    55441 agtaagtttg ccgcagcgta tgctgctttt tccctgacag cctcagaaag ggcgtcgtcg
    55501 gcagccattt cattcagcac tttcaaaacg cagcccagcg cgtccggaac gtatcctaag
    55561 tctccgctgg cgatttccgc gtaccgcttg cgtattaact cacaatattt ttccacatgc
    55621 cctcctgtca gcactctgac ttaaccgtgg atgcaagtct aagcctacga agataaactc
    55681 tgtttcgcaa ggtgactata ccacactcat ttctgcaata tcagcgccgc aactgcacgt
    55741 attccgttac aatggcctcc tgattcgaaa ggagttttct tatggcgctt aaagcgacaa
    55801 tttataaagc gacggttaat gtggccgatc tcgaccgcaa ccagtttctc gatacctctc
    55861 tgacgctggc gcgccatcct tcagaaaccc aggagcgcat gatgctgcgc ctgctggcgt
    55921 ggctaaaata tgccgatgaa cgtctgcaat ttacccgtgg tttgtgtgcc gatgatgagc
    55981 cggaagcgtg gctgcgtaac gatcatttgg gtattgattt gtggattgag ctggggctgc
    56041 cggatgagcg acggattaag aaagcctgca cccaggccgc agaagtggcg ctgtttgcct
    56101 ataatagtcg ggcggcgcaa atctggtggc agcaaaatca gagcaaatgt gcgcagtttg
    56161 ccaatctttc catctggtat ctggacgatg aacaactggc gaaagtaagc gcctttgccg
    56221 atcgtaccat gacgctgcag gcaacgattc aggatggcgt gatctggtta tcggatgata
    56281 agaataatct ggaagtgaac ttaaccgcct ggcaacaacc ttcatgattg tgatttcccg
    56341 acatgttgct atccccgatg gtgagcttga gatcaccgcc attcgtgcgc agggcgcggg
    56401 cgggcagcat gttaataaga cctcaacggc tattcatctg cgttttgaca ttcgggcgtc
    56461 cagcctgcca gagtattaca aagaacgtct gctcgccgcc agccatcact tgatcagcag
    56521 tgatggggtg attgtcatta aggcacagga ataccgcaat caggagctca accgcgaagc
    56581 ggcgctggcc cggctggtgg cagtgattaa agatttaaca acagaacaaa aagcccgacg
    56641 acccacgcgg cccacccgcg catcaaaaga gcgcaggctg gcatcgaaag cacaaaaatc
    56701 aagcgtgaag gcgatgcgcg gcaaagtgcg cagcggtcgg gaataaaaag aaggaatgga
    56761 tggtgaaaaa agcgatagtg acagcgatgg ctgtaatcag cctctttact ctgatgggat
    56821 gtaataatcg ggccgaagtc gatacgcttt ctccggcaca ggctgccgaa ctgaaaccga
    56881 tgccgcaaag ttggcgcggc gtgctgccgt gtgctgattg cgaaggaatc gaaacctctc
    56941 tgttcctcga aaaagacgga acctgggtga tgaatgagcg ttatctgggg gctcgtgaag
    57001 aaccttcctc cttcgcttcc tacggtacat gggcgcgaac cgctgacaag ctggtattaa
    57061 ccgatagcaa aggtgaaaag tcatattatc gcgcgaaagg agatgcgctg gagatgctcg
    57121 atcgtgaagg taatccgatt gaatcgcagt tcaactatac gctggaaccg gcacaatcca
    57181 gcttacccat gacaccgatg accctgcggg gcatgtattt ttatatggct gatgcggcga
    57241 ccttcactga ttgcgcgacc ggaaaacgtt tcatggtggc gaataacgca gagctggagc
    57301 gtggctacct ggctgcgcgc ggtaacagtg aaaaaccggt attactgtca gtagaaggtc
    57361 actttacgct tgaggctaat ccggataccg gtgcgccgac caaagtatta gcgcccgata
    57421 cggcaggtaa attttaccca aaccaggatt gcagtagttt ggggctgtaa cccgtcttga
    57481 gacagaaaca aatgaaatat tgcctcatgc gctgcgctta tcagtcctaa agcatacaat
    57541 gcaatatttt gaattagcac aattttgtag gccggataag gcgttcacgc cgcatccggc
    57601 attttgcgca cgaggctggc ttttatcact gagtcagccc aacaaagcgg cctgccttga
    57661 tataaatccc aggcttcatg tgcccaacat actgtaactg cgtttctggc ttaaacgcag
    57721 agatatcgcc agtacgcata tgcatcagat cggcagggct aatccagcct gattgcgcca
    57781 gcgtaagcgg atgcccggct ttggcaaatg catcagtgac aaattccgaa caaaaccacg
    57841 actttttgtc tccttcgccc acactgctta actgcgcttt cgccaggccg ctgacgcact
    57901 gttgacgaaa atcctcggag aacggattta gtgagcacat ctggcgagtc accataaatg
    57961 gaataaattc gacaatgccg cgatagttgt aaccgctatc tttaattttg ttggcgaacg
    58021 cggtgatttc tgtggcttgt tgcggggtaa gatccgggac tcgtaagacg aaaagcttat
    58081 cactatgctt tatggctttt ttaagggaaa caatctggac gccagcgcct gtcgcttcgg
    58141 caacgttatt atctcccaaa tagattgcaa cgtgactcac agaggaagtg ctgaatacgc
    58201 ggataccaaa tgaggttacc ccaaggcttg aggagaaaag caaatcgccg ggtttgagat
    58261 ctggttccgt tatttctttt attgattgtt cggtgaaaga gctttgatgc tggaatttaa
    58321 cagcccatgt tttcgcctcg gcatttacca ctgtggctga tgagtctggc tggctgatat
    58381 caacggtaca ggccgaaagt aaaagaaagc aggggagaag caggcggcag tacgcctttg
    58441 gtttatccat tttatacaat ccatgtaaaa aagggccccg aaattcagga ccctttctgg
    58501 catcagcctt taatctgttt caccagataa tcgacgatgt caccagtctt aattaactgt
    58561 ttctcgccgt tacgacgata tttatattcg atatcatcgt tgtcgaggtt acggtcgccc
    58621 agcacaatag tgtgcggaat accgatcagt tccatatcag caaacatcac gcccggacgc
    58681 tctttgcggt catccagcag cacttcgata ccttgtgcgc gcagttcgct gtacagtttc
    58741 tcagcaagtt cctgtacgcg gaaggatttg tgcatgttca ttggcagaat cgccacctgg
    58801 aacggcgcga tagcgtcagg ccatacgatg ccgcgttcgt cgtagttctg ctcaatcgcc
    58861 gcagctacca cacgcgttac gccgataccg tagcaaccca tcgtcaggat ttggttacgg
    58921 ccatcttcac cctgtacgga ggctttcagt gcttcggagt acttggtacc cagctggaag
    58981 atgtgaccga cttcgatacc acgtttgatc agcagcgtac cctgaccatc cgggcttgga
    59041 tcgccagcca ccacgttgcg gatatcagca atttccgggg tagcgacatc gcgatcccag
    59101 ttgataccga agtagtgttt accatcgatg ttagcaccag cagcgaaatc actcatcgcc
    59161 gcaacggtac ggtcaatcac caccggaatc ggcatgttta ccggacccag tgaacccgga
    59221 ccggctttaa ccacggcacg aatttcttct tcggtcgcaa aagtcagcgg gctggcaacc
    59281 tgcggcagtt tttctgcttt aacttcgttc agctcgtggt caccgcgcac cagcagcgca
    59341 accagcggga agctactgcc ttcaacggct ttaaccagca gagtcttaac cgttttctca
    59401 atcggcagat tgaactgttc aaccagttcc gcgatggtct tcgcgttcgg cgtatcaacc
    59461 agcgtcattt cctgggtagc agcagcgcgc ggttctttcg gcgcgatagc ttctgccagt
    59521 tcaatgttcg ctgcatagtc agaagtatcg gagaagacca catcgtcttc tccgctctgc
    59581 gccagcacct gaaattcgtg agaggcgcta ccgccgatag aaccggtatc ggcctgtacg
    59641 gcgcggaaat ccagccccat gcggctgaag attttgctgt aggccgcata cattgcatcg
    59701 taggtttcct gcagggattc ctgagaagta tggaaagagt aagcatcttt catcaggaat
    59761 tcgcgggaac gcatgacgcc gaaacgcgga cgcacttcgt cgcggaactt ggtctggatc
    59821 tgatagaagt tcagcggcag ctgtttgtaa gagctcagct cgttacgaat caggtctgta
    59881 atcacttctt catgagttgg gccgagtacg aacggacggt caccacggtc agcaatacgc
    59941 agcaattccg ggccatactg ttcccaacga ccactctctt gccacagttc agatggctga
    60001 accaccggca ttaacacctc gatcgcaccg gcgttgttca tctcttcacg cacgatgttt
    60061 tcgacttttt tcagaacgcg cacgccggtc ggcagccagg tatataaccc ggaggccagc
    60121 ttgcggatca tcccggcgcg cagcatcagc tgatggctga tcacctcggc gtcggcaggt
    60181 gtctccttga gagtggagag caggtattgg ctagtacgca tgttgttacg gttccagttg
    60241 gaaggtagaa caggctcaag gcgagcctgg gacaaaaaaa gtgatttagt ttaccagtgc
    60301 aaaagaaatg tcaaaagaga agggcgtgaa tttaacgcgg ttccagcgca aagacttcaa
    60361 aacctgcgtc ggtgacgcgc cagcgaacgt taaaatcatg cagccagacg gcataggttt
    60421 tgcccgtttc ctcaccttta cgataggccg ggcgcgggtc ctgcgccagt acttcgcgga
    60481 taaacagcgt taactgcgga taacgcttct tcagcgtcaa aagctgcttt tcgacctctg
    60541 cggtaaaaca caccgccatt tctgccgctg gcgcactttg cgcatagctg gcactggcat
    60601 cgggaagcga ttcagcaaag gggagatacg gtttgatatc cactaccggc gtaccatcga
    60661 ccagatccag actgccgagc ttcagaatca cgctgtcttt atggcaaaca acctctttca
    60721 gctctaccag cgacatacca atcgggttag ggcgaaaagt ggagcgcgta gcgaaaacac
    60781 ccattctggc attaccgccg agacgaggcg gacgcacagt cggacgccag ccgccttcca
    60841 tcgtttgatg aaagacgaaa aggatccata aatggctgaa tgcttccagg ccgcgaacgg
    60901 cgtcggcctg gttgtaggga gcaatgagat gtagttcgcc gttggcgctt tttaccagac
    60961 ctggctggcg cggaacggcg aatttttctt tatagggcga gcgaataacg cctatttgct
    61021 cgaactggaa actgctcatt tcgccgtgat gttaagcgca gaaccgatac atacagcctg
    61081 acgatagcaa cctggcgtac cgctggtgac ttcgcagcta tgcagtaata ccgcattggc
    61141 tttcattttc gaggcgttga tttgcatccg cttacgtgcg gttggaatgc tcggcggaga
    61201 gtcctgatta gaggcctggc aagagtcgcc actgacttca ccgagatcgc ggaacggttt
    61261 gccgactaat tcttctgcat tggtataaat tcggaccggc gtggcgcgcg gcgctttcgg
    61321 ttttgcaggc tccgctttcg gctggggtgc agtgctttga acgggttcga caggggatct
    61381 gcttaacatg gaacagccgc ttagcatgag tgctactaaa cagatcggta aagcacgcat
    61441 agtatttcct caatgtatga tcaaaacgtc aatattgaat caggcgcttg taaaaatgac
    61501 aagacgggca agcgcccgtc ctgaatgata ttacaaattg tggaaacagc ctaaatatta
    61561 ccagccttta acagcgccgc cgttaaacac tttgtttgct gcttcgtaaa cttcgtcaga
    61621 ctgataagcc tgaacgaatt tcttcacgtt ttcggcgtct ttgttgtctt cacgcgttac
    61681 gatcaggttt acgtacgggg actctttatc ttcgacaaag ataccgtctt tcgctggagt
    61741 caggccaatc tggctggcat aggtggtatt gataactgcc agagcgattt gcgcgtcgtc
    61801 cagagagcgc ggcagctgcg gtgcttccag ttcaacaatt ttcagatttt ttgggttctc
    61861 aacaacatca agaacggtcg gcagcaggcc aacgccatct ttcagtttga tcaagcccac
    61921 tttttgcagc agcagtagtg aacgaccaag gttagttggg tcgtttggca cggcaacctg
    61981 cgaaccatcc tgcagttcat ccagtgattt gattttcttg gagtaaccag caatcggata
    62041 aacaaatgtg ttgcctactg cgaccagttt gtagccacga tctttcagtt gctgatcaag
    62101 gtacggttta tgctggaagg cgttggcgtc gatatcgcct ttgctcaatg cttcgtttgg
    62161 cagaacatag tcgttgaagg ttaccagctc aacgtccagg ccatatttgt ctttcgcaac
    62221 tttctgcgcg acttctgcaa cctgctgttc ggcaccaaca atcacgccga ctttaatgtg
    62281 gtttggatct ttttcatcct gaccgcagcc taccagtgcc agtgatccaa tcagggctcc
    62341 cactgccgca aaggttttga atttgaacgc cataccttat tccttcttca attatttatg
    62401 ttgtgttgaa cgttacttgc gagtgacagc ccggacgatg cggtcgcctg cgaactgaat
    62461 taaataaacc agaatgacca gcaataccag taccgtattc atcaccgtcg cgttgtagcc
    62521 gatgtagcca tactgatagc caatctgacc taaaccaccg gcaccgactg caccacccat
    62581 cgcggaataa ccaaccaggg taatcagggt gatagttgcc gcatttacca gacccggcag
    62641 cgcttccggt aacagcacct tacggacgat ctgcatcggc gtggcaccca ttgcgcggga
    62701 agcttcaatt aacccggttg ggatctccag cagagcgttc tcgaccatac gggcaataaa
    62761 cggtgctgca ccaacggtta atggaacgat cgctgcctgc aaaccaatcg atgtaccgac
    62821 aataacgcgg gtaaacggaa tcatccatac cagcaagata atgaacggga tggaacggaa
    62881 aatgttcaca atcgcagaaa cggtacgata cagcttcgcg ttagcaataa tttgccccgg
    62941 acgcgtgaca taaagcagaa cgccaaccgg cagaccaatc acaaagccaa aaaaaccgga
    63001 tacgaaggtc attgccagcg tttcccatac gccacgaacc agcagccaca tcatcggctc
    63061 agacataacc cagtacctct acttttacat ggtgttcctg cagccaggca atggcggctt
    63121 gcgtatcttg ttgtgtgccg tgcatttcag tcagcatgat gccgaactta acgccaccgg
    63181 cgtaatccat ctgcgcgcta ataatgttgt tgttgacgtt gaaacgacgc gcggtttcag
    63241 aaagcagtgg ggcatcgacc gattgaccgg taaactccag acgcagcatc ggcacgcagt
    63301 cagtgaatgg ttccgcttgc agacgctcct gataatcttc cgggatatcc agatgcaggg
    63361 tcgactgaat aaacttctgc gccagcggcg ttttcggatg cgagaacact tcacttaccg
    63421 tgtcctgctc gatcagttcg ccattactga taaccgccac gcaatcacaa atgcgcttca
    63481 caacgtccat ttcgtgggtg atcaacagaa tcgtcaaacc cagacggcgg ttgatgtctt
    63541 taagcagttc gagaatagaa cgtgtcgttg ccgggtccag cgcgctggtg gcttcatcac
    63601 acagcaatac tttaggattg ctggctaacg cacgggcaat cgccacacgc tgtttctgcc
    63661 caccggaaag gttcgacgga tagctatcat gcttatcacc aagaccaacc aatgacagca
    63721 attccgtcac gcgacgtttg atttcgtctt tcggcgtgtt gtccagctcc agcggcagag
    63781 ccacgttgcc aaaaacagta cgcgaagaga gcaggttaaa atgctggaaa atcataccaa
    63841 tctggcggcg agctttagtc aactcagatt ctgacagcgt ggtcagctcc tggccatcaa
    63901 ccagcacgct accctcggtt gggcgctcca gcaggtttac acaacgtata agcgtactct
    63961 tacccgcgcc tgaggcaccg ataacgccat aaatttgtcc agctggcaca tgcaggctga
    64021 cgttgttcaa cgcctggatg gtgcgggtgc cctggtggaa cactttggtg atattcgaaa
    64081 gttttatcat tgattattta ttatcgtcat taagttagtc gtggcatttc gaatgcctgg
    64141 aacgggcaac gccgtcaatg aaatggatgt taaggcatcc agacgtctaa atcaatcagg
    64201 tttatgcgaa gagcactttc ttgcaggtcg aaacatgcga tactagcgtc acatgcctta
    64261 ttaaggagct ataaaaggtg gcgaagagcg tacccgcaat ttttcttgac cgtgatggca
    64321 ccattaatgt cgatcacggt tatgtccatg agatcgacaa ctttgaattt atcgacggtg
    64381 ttattgacgc catgcgcgag ctaaaaaaaa tgggctttgc gctggtggta gtaaccaacc
    64441 agtctggcat tgctcgcggt aaatttactg aagcacagtt tgaaacgctg accgagtgga
    64501 tggactggtc gctggcggac cgcgatgtcg atctggatgg tatctattat tgcccgcatc
    64561 atccgcaggg tagtgttgaa gagtttcgcc aggtctgcga ctgccgcaaa ccacatccgg
    64621 ggatgttttt gtcagcacgc gattatttgc atattgatat ggccgcttct tatatggtgg
    64681 gcgataaatt agaagatatg caggcagcgg ctgcggcgag cgtgggaaca aaagtgctgg
    64741 tgcgtacggg taaacctatt acgcctgaag cagaaaacgc ggcggattgg gtgttaaata
    64801 gcctggcaga cctgccgcaa gcgataaaaa agcagcaaaa accggcgtaa tgattaaaag
    64861 atgagcggtt gaaataaaaa tgcatttttc cgcttgtctt cctgagacga ctccctataa
    64921 tgcgcctcca tcgacacggc ggatgtgaat cacttcacac aaacagccgg ttcggttgaa
    64981 ga
//
LOCUS       DADSYM010000003        62460 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2415
            SAMN08039579-rid11723173.denovo.003, whole genome shotgun sequence.
ACCESSION   DADSYM010000003 DADSYM010000000
VERSION     DADSYM010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039579
            Sequence Read Archive: SRR6362533
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 62460)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 62460)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980162.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 03:39:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,484
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 4,347
            CDSs (with protein)               :: 4,347
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 43 of 85
            Pseudo Genes (incomplete)         :: 37 of 85
            Pseudo Genes (internal stop)      :: 19 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..62460
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039579-rid11723173.denovo.003"
                     /strain="LGE2415"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8302 N 95.9628 W"
                     /collection_date="2015-08-24"
     gene            185..349
                     /locus_tag="J1Y83_000128"
     CDS             185..349
                     /locus_tag="J1Y83_000128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmiA family putative membrane protein"
                     /protein_id="HBA7628322.1"
                     /translation="MLISDGDYMRLAMPSGNQEPRRNPELKRKAWLAVFLGSALFWVV
                     VALLIWKLWG"
     gene            352..519
                     /locus_tag="J1Y83_000129"
     CDS             352..519
                     /locus_tag="J1Y83_000129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_304581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA7628323.1"
                     /translation="MVGQEQLESSPLCQHSDNEPEAKRECSVVIPDDWQLTSQQQAFI
                     ELFAEDDQPKQ"
     gene            633..728
                     /locus_tag="J1Y83_000130"
     CDS             633..728
                     /locus_tag="J1Y83_000130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA7628324.1"
                     /translation="MINTNMKYWSWMGAFSLSMLFWAELLWIITH"
     gene            878..3553
                     /gene="acnA"
                     /locus_tag="J1Y83_000131"
     CDS             878..3553
                     /gene="acnA"
                     /locus_tag="J1Y83_000131"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase AcnA"
                     /protein_id="HBA7628325.1"
                     /translation="MSSTLREASKDTLQAKDKTYHYYSLPLAAKSLGDITRLPKSLKV
                     LLENLLRWQDGKSVNEEDIHALAGWLKNAHADREIAYRPARVLMQDFTGVPAVVDLAA
                     MREAVKRLGGDTAKVNPLSPVDLVIDHSVTVDRFGDDEAFEENVRLEMERNHERYVFL
                     KWGKQAFSRFSVVPPGTGICHQVNLEYLGKAVWSELQDGEWIAYPDTLVGTDSHTTMI
                     NGLGVLGWGVGGIEAEAAMLGQPVSMLIPDVVGFKLTGKLREGITATDLVLTVTQMLR
                     KHGVVGKFVEFYGDGLDSLPLADRATIANMSPEYGATCGFFPIDAVTLDYMRLSGRSE
                     DQVELVEKYAKAQGMWRNPGDEPIFTSVLELDMNDVEASLAGPKRPQDRVALPDVPKA
                     FAASSELEVNATHKDRLPVDYVMNGHQYQLPDGAVVIAAITSCTNTSNPSVLMAAGLL
                     AKKAVTLGLKRQPWVKASLAPGSKVVSDYLAKAKLTPYLDELGFNLVGYGCTTCIGNS
                     GPLPDPIETAIKKGDLTVGAVLSGNRNFEGRIHPLVKTNWLASPPLVVAYALAGNMNI
                     NLASEPIGHDRKGEPVYLKDIWPSAQGIARAVDQVSTEMFRKEYAEVFEGTAEWKEIN
                     VTRSDTYGWQEDSTYIRLSPFFDEMQATPAPVEDIHGARILAMLGDSVTTDHISPAGS
                     IKPDSPAGRYLQGRGVERKDFNSYGSRRGNHEVMMRGTFANIRIRNEMVPGVEGGMTC
                     HLPDSDVVSIYDAAMRYKQEQTPLAVIAGKEYGSGSSRDWAAKGPRLLGIRVVIAESF
                     ERIHRSNLIGMGILPLEFPQGVTRKTLELTGEEKIDIGDLQNLQPGATVPVTLTRADG
                     SQEVVPCRCRIDTATELTYYQNDGILHYVIRNMLK"
     gene            complement(3617..4207)
                     /gene="ribA"
                     /locus_tag="J1Y83_000132"
     CDS             complement(3617..4207)
                     /gene="ribA"
                     /locus_tag="J1Y83_000132"
                     /EC_number="3.5.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase II"
                     /protein_id="HBA7628326.1"
                     /translation="MQLKRVAEAKLPTPWGDFLMVGFEELATGHDHVALVYGDISGHT
                     PVLARVHSECLTGDALFSLRCDCGFQLEAALTQIAEEGRGILLYHRQEGRNIGLLNKI
                     RAYALQDQGYDTVEANHQLGFAADERDFTLCADMFKLLGVNEVRLLTNNPKKVEILTE
                     AGINIIERVPLIVGRNPNNEHYLDTKAEKMGHLLNK"
     gene            4377..5141
                     /gene="pgpB"
                     /locus_tag="J1Y83_000133"
     CDS             4377..5141
                     /gene="pgpB"
                     /locus_tag="J1Y83_000133"
                     /EC_number="3.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase B"
                     /protein_id="HBA7628327.1"
                     /translation="MRSIARRTAVGAALLLVMPVAVWLSGWRWQPGEQSWLLKAAFWV
                     TETVTQPWGVITHLILFGWFLWCLRFRIKAAIMLFAILAAAILMGQGVKSWIKDKVQE
                     PRPFVIWLEKTHHIPVDEFYTLKRAERGNLVKEQLAEEKNIPQYLRSHWQKETGFAFP
                     SGHTMFAASWALLAVGLLWPRRRTLTIAILLVWATGVMGSRLLLGMHWPRDLVVATLI
                     SWALVAVATWLAQRICGPLTPPAEENREIAQREQES"
     gene            5290..5598
                     /gene="lapA"
                     /locus_tag="J1Y83_000134"
     CDS             5290..5598
                     /gene="lapA"
                     /locus_tag="J1Y83_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapA"
                     /protein_id="HBA7628328.1"
                     /translation="MKYLLIFLLVLAIFVISVTLGAQNDQQVTFNYLLAQGEYRISTL
                     LAVLFAAGFAIGWLICGLFWLRVRVSLARAERKIKRLENQLSPATDVAVAPHSSASKE
                     "
     gene            5605..6774
                     /gene="lapB"
                     /locus_tag="J1Y83_000135"
     CDS             5605..6774
                     /gene="lapB"
                     /locus_tag="J1Y83_000135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapB"
                     /protein_id="HBA7628329.1"
                     /translation="MLELLFLLLPVAAAYGWYMGRRSAQQNKQDEANRLSRDYVAGVN
                     FLLSNQQDKAVDLFLDMLKEDTGTVEAHLTLGNLFRSRGEVDRAIRIHQTLMESASLT
                     YEQRLLAIQQLGRDYMAAGLYDRAEDMFNQLTDETDFRIGALQQLLQIYQATSEWQKA
                     IDVAERLVKLGKDKQRVEIAHFYCELALQHMASDDLDRAMTLLKKGAAADKNSARVSI
                     MMGRVFMAKGEYAKAVESLQRVISQDRELVSETLEMLQTCYQQLGKTAEWAEFLQRAV
                     EENTGADAELMLADIIEARDGSEAAQVYITRQLQRHPTMRVFHKLMDYHLNEAEEGRA
                     KESLMVLRDMVGEKVRSKPRYRCQKCGFTAYTLYWHCPSCRAWSTIKPIRGLDGL"
     gene            6965..7702
                     /gene="pyrF"
                     /locus_tag="J1Y83_000136"
     CDS             6965..7702
                     /gene="pyrF"
                     /locus_tag="J1Y83_000136"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HBA7628330.1"
                     /translation="MTLTASSSSRAVTNSPVVVALDYHNRDAAMAFVDKIDPRDCRLK
                     VGKEMFTLFGPQFVSELQQRGFDIFLDLKFHDIPNTAAHAVAAAADLGVWMVNVHASG
                     GARMMAAAREALVPFGKDAPLLIAVTVLTSMEASDLADLGVTLSPADYAERLAALTQK
                     CGLDGVVCSAQEAVRFKQVFGQEFKLVTPGIRPQGSDAGDQRRIMTPEQALSAGVDYM
                     VIGRPVTQSVDPAQTLKAINASLQRSA"
     gene            7702..8028
                     /gene="yciH"
                     /locus_tag="J1Y83_000137"
     CDS             7702..8028
                     /gene="yciH"
                     /locus_tag="J1Y83_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response translation initiation inhibitor
                     YciH"
                     /protein_id="HBA7628331.1"
                     /translation="MSDSNSRLVYSTETGRIDEPKAAPVRPKGDGVVRIQRQTSGRKG
                     KGVCLITGVDLDDAELTKLAAELKKKCGCGGAVKDGIIEIQGDKRDLLKSMLEAKGMK
                     VKLAGG"
     gene            complement(8154..8372)
                     /gene="osmB"
                     /locus_tag="J1Y83_000138"
     CDS             complement(8154..8372)
                     /gene="osmB"
                     /locus_tag="J1Y83_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotically-inducible lipoprotein OsmB"
                     /protein_id="HBA7628332.1"
                     /translation="MFVTSKKMTAAVLAITLAMSLSACSNWSKRDRNTAIGAGAGALG
                     GAVLTDGSTLGTLGGAAVGGVIGHQVGK"
     gene            complement(8641..9390)
                     /locus_tag="J1Y83_000139"
     CDS             complement(8641..9390)
                     /locus_tag="J1Y83_000139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HBA7628333.1"
                     /translation="MNSRQQTILQMVIDQGQVSVTDLAKATGVSEVTIRQDLNTLEKL
                     SYLRRAHGFAVSLDSDDVETRMMSNYTLKRELAEFAASLVQPGETIFIENGSSNALLA
                     RTLGEQKKNATIITVSSYIAHLLKDAPCEVILLGGVYQKKSESMVGPLTRQCIQQVHF
                     SKAFIGIDGWQPETGFTGRDMMRTDVVNAVLEKECEAIVLTDSSKFGAVHSYSIGPVE
                     RFNRVITDSKIRASDLMHLEHSKLTVHVVDI"
     gene            complement(9480..9653)
                     /locus_tag="J1Y83_000140"
     CDS             complement(9480..9653)
                     /locus_tag="J1Y83_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA7628334.1"
                     /translation="MSEFDAQRVAERIDIVLDILVAGDYHSAIHNLEILKAELLRQVA
                     ESTPDIPKAPWEI"
     gene            complement(9801..11786)
                     /gene="pdeR"
                     /locus_tag="J1Y83_000141"
     CDS             complement(9801..11786)
                     /gene="pdeR"
                     /locus_tag="J1Y83_000141"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-GMP phosphodiesterase"
                     /protein_id="HBA7628335.1"
                     /translation="MKTVRESATLYNFLGSHNPYWRLTESSDVLRFSTTEITEPDRIL
                     QLSAEQAARIREMTVITSSLMMSLTVDESDLSVHLVGRKINKREWGGNASAWHDTPAV
                     ARDLSHGLSFAEQVVSEAHSAIVILDSRGNIQRFNRLCEDYTGLKEHDVIGQSVFKLF
                     MSRREAAASRRNNRVFFRSGNAYEVELWIPTRKGQRLFLFRNKFVHSGSGKNEIFLIC
                     SGTDITEERRAQERLRILANTDSITGLPNRNAMQELIDHAINQADNNKVGVVYLDLDN
                     FKKVNDAYGHLFGDQLLRDVSLAILSCLEHDQVLARPGGDEFLVLASNTSQSALEALA
                     SRILTRLRLPFRIGLIEVYTSCSVGISLSPEHGSDSAAIIRHADTAMYTAKEGGRGQF
                     CVFTPEMNQRVFEYLWLDTNLRKALENDQLVIHYQPKITWRGEVRSLEALVRWQSPER
                     GLIPPLDFISYAEESGLIVPLGRWVILDVVRQVAKWRDKGINLRVAVNISARQLADQT
                     IFTALKQVLQELNFEYCPIDVELTESCLIENDELALSVIQQFSRLGAQVHLDDFGTGY
                     SSLSQLARFPIDAIKLDQVFVRDIHKQPVSQSLVRAIVAVAQALNLQVIAEGVESAKE
                     DAFLTKNGINERQGFLFAKPMPAVAFERWYKRYLKRA"
     gene            11840..11943
                     /locus_tag="J1Y83_000142"
                     /pseudo
     CDS             11840..11943
                     /locus_tag="J1Y83_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791239.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(12020..13954)
                     /gene="rnb"
                     /locus_tag="J1Y83_000143"
     CDS             complement(12020..13954)
                     /gene="rnb"
                     /locus_tag="J1Y83_000143"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exoribonuclease II"
                     /protein_id="HBA7628336.1"
                     /translation="MFQDNPLLAQLKQQLHSQTPRAEGVVKATEKGFGFLEVDAQKSY
                     FIPPPQMKKVMHGDRIIAVIHSEKERESAEPEELVEPFLTRFVGKVQGKNDRLAIVPD
                     HPLLKDAIPCRAARGLNHEFKEGDWAVAEMRRHPLKGDRSFYAELTQYITFGDDHFVP
                     WWVTLARHNLEKEAPDGVATEMLDEGLVREDLTALDFVTIDSASTEDMDDALFAKALP
                     DGKLQLIVAIADPTAWIAEGSKLDKAAKIRAFTNYLPGFNIPMLPRELSDDLCSLRAN
                     EVRPVLACRMTLSTDGTIEDNIEFFAATIESKAKLVYDQVSDWLENTGDWQPESEAIA
                     EQVRLLAQICQRRGEWRHNHALVFKDRPDYRFILGEKGEVLDIVAEPRRIANRIVEEA
                     MIAANICAARVLRDKLGFGIYNVHMGFDPANADALAALLKTHGLHVDAEEVLTLDGFC
                     KLRRELDAQPTGFLDSRIRRFQSFAEISTEPGPHFGLGLEAYATWTSPIRKYGDMINH
                     RLLKAVIKGETATRPQDEITVQMAERRRLNRMAERDVGDWLYARFLKDKAGTDTRFAA
                     EIVDISRGGMRVRLVDNGAIAFIPAPFLHAVRDELVCSQENGTVQIKGETAYKVTDVI
                     DVTIAEVRMETRSIIARPVA"
     gene            complement(14022..15149)
                     /locus_tag="J1Y83_000144"
     CDS             complement(14022..15149)
                     /locus_tag="J1Y83_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707196.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CMD domain-containing protein"
                     /protein_id="HBA7628337.1"
                     /translation="MEQRHITGKSHWYHETQSSTAEYDVLPLVPEAAKVSDPFLLDVI
                     LDEETLAPFLSWLVPARLLAVELFPDQLTVTRSQTFTAYERLSTALTVAQVCGVQRLC
                     NYYSARLTPLPGPDSSRESNHRLAQITQYARQLASSPSIIDNRSRQHLNDVGLTACDC
                     VIINQIIGFIGFQARTIATFQAYLGHPVRWLPGLEIQNYADASLFADESLRWRSSYEV
                     EKLPEEYTKSSTAELCQLTETLSLHPISLSLLEMLLNSTRVKTQPGNLLAALLCARIN
                     GSPACFAACMNSSNEYKKISLLLRKGENEINRWADRHSVERATVQAIQWLTRAPDRFS
                     AAQFSPLLEHEKSSTQIINLLVWSGLCGWINRLKIALGETY"
     gene            complement(15294..16082)
                     /gene="fabI"
                     /locus_tag="J1Y83_000145"
     CDS             complement(15294..16082)
                     /gene="fabI"
                     /locus_tag="J1Y83_000145"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HBA7628338.1"
                     /translation="MGFLSGKRILVTGVASKLSIAYGIAQAMHREGAELAFTYQNDKL
                     KGRVEEFAAQLGSDIVLQCDVAEDTSIDTMFAELGKVWPKFDGFVHSIGFAPGDQLDG
                     DYVNAVTREGFKIAHDISSYSFVAMAKACRSMLNPGSALLTLSYLGAERAIPNYNVMG
                     LAKASLEANVRYMANAMGPEGVRVNAISAGPIRTLAASGIKDFRKMLAHCEAVTPIRR
                     TVTIEDVGNSAAFLCSDLSAGISGEVVHVDGGFSIAAMNELELK"
     gene            16560..17126
                     /locus_tag="J1Y83_000146"
     CDS             16560..17126
                     /locus_tag="J1Y83_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBA7628339.1"
                     /translation="MTSKLEIRHKQRQDEIINAARQCFRRCGFHAASMSQIASEAQLS
                     VGQIYRYFANKDAIIEEMVRRIIDFRIAQMDIDARTDHFPEVLALRKSLNEDDDALML
                     EVAAEATRNPRVMAMLEEADARMFANGCAHMKRMHPHLSDEHIRCCVEVFATMMEGTV
                     YRRLTPQKSDPQHLQEIYQDIVSMLINK"
     gene            17275..18396
                     /locus_tag="J1Y83_000147"
     CDS             17275..18396
                     /locus_tag="J1Y83_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HBA7628340.1"
                     /translation="MKYIATSVVAMLLLSGCDNTQSNNSSPSETEVGVVTLKSQPVSV
                     VSELTGRTSAALSAEVRPQVGGIIQKRLFKEGDLVKAGQPLYQIDAASYQAAWNEARA
                     ALQQAQALVKADCQKAQRYARLVKENGVSQQDADDAQSTCAQDKASVEAKKAALETAR
                     INLDWTTVTAPISGRIGISSVTPGALVTASQDTALTTIRGLDTMYVDLTRSSVDLLRL
                     RKQSLATNSDTMSVSLILEDGTTYSEKGRLELTEVAVDESTGSVTLRAIFPNPQQQLL
                     PGMFVRARVDEGVMEDAILAPQQGVTRDAKGNATALVVNKDNKVEQRTLETGETYGDK
                     WLVLNGLHNGDRLIVEGSAKVTSGQTVKAVEVQANGGNA"
     gene            18396..21484
                     /locus_tag="J1Y83_000148"
                     /pseudo
     CDS             18396..21484
                     /locus_tag="J1Y83_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309891.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
     gene            21507..22880
                     /locus_tag="J1Y83_000149"
     CDS             21507..22880
                     /locus_tag="J1Y83_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HBA7628341.1"
                     /translation="MLRRSLIFLVLLSAGCVSLDPHYSTPESPIPATLPGAQGQGKAI
                     SHDWQQVIHDPRLQQVVTIALNSNRDVQKAIADIDSARALYGQTNASLFPTVNAALSS
                     TRSRSLANGTGTTAEADGTVSSYTLDLFGRNQSLSRAARETWLASEFTAQNTRLTLIA
                     EISTAWLTLAADNSNLALAKETMASAENSLKIIQRQQQVGTAAATDVSEAMSVYQQAR
                     ASVASYQTQVMQDKNALNLLAGTTLAENLLPGTLESLPEQMISLVPAGVSSDVLLRRP
                     DIQEAEHNLKSANADIGAARANFFPTISLTASAGVGSDALSSLFSHGMQIWSFAPSVT
                     LPLFTGGSNLAQLRYAEAQKRGLIATYEKTVQSAFKDVANALARRTTLEEQLDAQRQY
                     VKAEQQTVDVGLRRYQAGVGDYLTVLTAQRSLWSAQQELLALQLTDFTNRITLWQSLG
                     GGMSSLK"
     gene            22889..24052
                     /locus_tag="J1Y83_000150"
     CDS             22889..24052
                     /locus_tag="J1Y83_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug effflux MFS transporter"
                     /protein_id="HBA7628342.1"
                     /translation="MARVSLSWALILGLLAGIGPMCTDLYLPALPEMSEQLAATTTIT
                     QLTLTASLIGLGVGQLLFGPLSDKIGRKRPLILSLLLFIVSSILCATTNNIYWLVVWR
                     FIQGIAGAGGSVLSRSIARDKYQGVTLTQFFALLMTVNGLAPVLSPVLGGYIVSTFDW
                     RTLFWVMAEISTVLLLGCLLFINETLPENKRGSSLLLTGRSVVQNRRFMRFCLIQSFM
                     LAGLFAYIGSSSFVLQKEFGFSPMQFSLVFGLNGIGLIIASWIFSRLARRINAMILLR
                     GGLIAAILCALLTVLCAWVQLPIPALVALFFTIAFCSGIGTVGGAEAMSAVGTQESGT
                     ASALMGMSMFVFGGIAAPLSGIGGETLLKMSLAITVCYTLALLVALTRIDNQK"
     gene            complement(24100..24906)
                     /gene="sapF"
                     /locus_tag="J1Y83_000151"
     CDS             complement(24100..24906)
                     /gene="sapF"
                     /locus_tag="J1Y83_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter ATP-binding protein
                     SapF"
                     /protein_id="HBA7628343.1"
                     /translation="MIETLLEVRNLSKTFRYRTGWFRRQTVEAVKPLSFTLREGQTLA
                     IIGENGSGKSTLAKMLAGMIEPTSGELLIDDHPLHFGDYSFRSQRIRMIFQDPSTSLN
                     PRQRISQILDFPLRLNTDLEPEQRRKQIIETMRMVGLLPDHVSYYPHMLAPGQKQRLG
                     LARALILRPKVIIADEALASLDMSMRSQLINLMLELQEKQGISYIYVTQHIGMMKHIS
                     DQVLVMHQGEVVERGSTADVLASPLHELTKRLIAGHFGEALTADAWRKDR"
     gene            complement(24908..25900)
                     /gene="sapD"
                     /locus_tag="J1Y83_000152"
     CDS             complement(24908..25900)
                     /gene="sapD"
                     /locus_tag="J1Y83_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter ATP-binding protein
                     SapD"
                     /protein_id="HBA7628344.1"
                     /translation="MPLLDIRNLTIEFKTGDEWVKAVDRVSMTLTEGEIRGLVGESGS
                     GKSLIAKAICGVNKDNWRVTADRMRFDDIDLLRLSARERRKLVGHNVSMIFQEPQSCL
                     DPSERVGRQLMQNIPAWTYKGRWWQRFGWRKRRAIELLHRVGIKDHKDAMRSFPYELT
                     EGECQKVMIAIALANQPRLLIADEPTNSMEPTTQAQIFRLLTRLNQNSNTTILLISHD
                     LQMLSQWADKINVLYCGQTVETAPSKELVTMPHHPYTQALIRAIPDFGSAMPHKSRLN
                     TLPGAIPLLEQLPIGCRLGPRCPYAQRECIVTPRLTGAKNHLYACHFPLNMEKE"
     gene            complement(25900..26790)
                     /gene="sapC"
                     /locus_tag="J1Y83_000153"
     CDS             complement(25900..26790)
                     /gene="sapC"
                     /locus_tag="J1Y83_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter permease SapC"
                     /protein_id="HBA7628345.1"
                     /translation="MPYDSVYSEKRPPGTLRTAWRKFYSDASAMVGLYGCAGLAVLCI
                     FGGWFAPYGIDQQFLGYQLLPPSWSRYGEVSFFLGTDDLGRDVLSRLLSGAAPTVGGA
                     FVVTLAATICGLVLGTFAGATHGLRSAVLNHILDTLLAIPSLLLAIIVVAFAGPSLSH
                     AMFAVWLALLPRMVRSIYSMVHDELEKEYVIAARLDGASTLNILWFAVMPNITAGLVT
                     EITRALSMAILDIAALGFLDLGAQLPSPEWGAMLGDALELIYVAPWTVMLPGAAIMIS
                     VLLVNLLGDGVRRAIIAGVE"
     gene            complement(26777..27742)
                     /gene="sapB"
                     /locus_tag="J1Y83_000154"
     CDS             complement(26777..27742)
                     /gene="sapB"
                     /locus_tag="J1Y83_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter permease SapB"
                     /protein_id="HBA7628346.1"
                     /translation="MIIFTLRRILLLIVTLFLLTFVGFSLSYFTPHAPLQGASLWNAW
                     VFWFNGLIHWDFGVSSINGQPIAEQLKEVFPATMELCILAFGFALIVGIPVGMIAGIT
                     RHKWQDNLINAIALLGFSIPVFWLALLLTLFCSLTLGWLPVSGRFDLLYEVKPITGFA
                     LIDAWLSNSPWRDEMVMSAIRHMILPVITLSVAPTTEVIRLMRISTIEVYDQNYVKAA
                     ATRGLSRFTILRRHVLHNALPPVIPRLGLQFSTMLTLAMITEMVFSWPGLGRWLINAI
                     RQQDYAAISAGVMVCGSLVIIVNVISDILGAMANPLKHKEWYALR"
     gene            complement(27739..29382)
                     /gene="sapA"
                     /locus_tag="J1Y83_000155"
     CDS             complement(27739..29382)
                     /gene="sapA"
                     /locus_tag="J1Y83_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein SapA"
                     /protein_id="HBA7628347.1"
                     /translation="MRQVLSSLLVIAGLVSGQAIAAPESPPHADIRDSGFVYCVSGQV
                     NTFNPSKASSGLIVDTLAAQFYDRLLDVDPYTYRLMPELAESWEVLDNGATYRFHLRR
                     DVPFQKTDWFTPTRKMNADDVVFTFQRIFDRNNPWHNVNGSNFPYFDSLQFADNVKSV
                     RKLDNHTVEFRLAQPDASFLWHLATHYASVMSAEYAGKLEKEDRQEQLDRQPVGTGPY
                     QLSEYRAGQYIRLQRHDDFWRGKPLMPQVVVDLGSGGTGRLSKLLTGECDVLAWPAAS
                     QLSILRDDPRLRLTLRPGMNVAYLAFNTAKPPLNNPAVRHALALAINNQRLMQSIYYG
                     TAETAASILPRASWAYDNEAKITEYNPAKSREQLKALGLENLTLKLWVPTRSQAWNPS
                     PLKTAELIQADMAQVGVKVVIVPVEGRFQEARLMDMSHDLTLSGWATDSNDPDSFFRP
                     LLSCAAIHSQTNLAHWCDPKFDSVLRKALSSQQLAARIEAYDEAQSILAQELPILPLA
                     SSLRLQAYRYDIKGLVLSPFGNASFAGVYREKQDEVKKP"
     gene            complement(29695..29940)
                     /locus_tag="J1Y83_000156"
     CDS             complement(29695..29940)
                     /locus_tag="J1Y83_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmjA family protein"
                     /protein_id="HBA7628348.1"
                     /translation="MNHDIPLKYFDIADEYATECAEPVADAERTPLAHYFQLLLTRLM
                     NNEEISEEAQHEMAAEAGINPVRIDEIAEFLNQWGNE"
     gene            complement(30074..30181)
                     /locus_tag="J1Y83_000157"
                     /pseudo
     CDS             complement(30074..30181)
                     /locus_tag="J1Y83_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415812.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine importer"
     gene            complement(30365..31342)
                     /gene="pspF"
                     /locus_tag="J1Y83_000158"
     CDS             complement(30365..31342)
                     /gene="pspF"
                     /locus_tag="J1Y83_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein operon transcriptional
                     activator"
                     /protein_id="HBA7628349.1"
                     /translation="MAEYKDNLLGEANSFLEVLEQVSHLAPLDKPVLIIGERGTGKEL
                     IASRLHYLSSRWQGPFISLNCAALNENLLDSELFGHEAGAFTGAQKRHPGRFERADGG
                     TLFLDELATAPMMVQEKLLRVIEYGELERVGGSQPLQVNVRLVCATNADLPAMVNEGT
                     FRADLLDRLAFDVVQLPPLRERESDIMLMAEHFAIQMCREIKLPLFPGFTEHARETLL
                     NYRWPGNIRELKNVVERSVYRHGTSDYPLDDIIIDPFKRRPSEEAIAVSENTSLPTLP
                     LDLREFQMQQEKELLQLSLQQGKYNQKRAAELLGLTYHQFRALLKKHQI"
     gene            31509..32177
                     /gene="pspA"
                     /locus_tag="J1Y83_000159"
     CDS             31509..32177
                     /gene="pspA"
                     /locus_tag="J1Y83_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein PspA"
                     /protein_id="HBA7628350.1"
                     /translation="MGIFSRFADIVNANINALLEKAEDPQKLVRLMIQEMEDTLVEVR
                     STSARALAEKKQLTRRIEQASAREIEWQEKAELALLKDREDLARAALIEKQKLTDLIK
                     SLEHEVTLVDDTLVRMKKEIGELENKLSETRARQQALMLRHQAANSSRDVRRQLDSGK
                     LDEAMARFESFERRIDQMEAEAESHSFGKQKSLDDQFAELKADDAISEQLAQLKAKMK
                     QDNQ"
     gene            32231..32455
                     /gene="pspB"
                     /locus_tag="J1Y83_000160"
     CDS             32231..32455
                     /gene="pspB"
                     /locus_tag="J1Y83_000160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response membrane protein PspB"
                     /protein_id="HBA7628351.1"
                     /translation="MSALFLAIPLTIFVLFVLPIWLWLHYSNRSGRSELSQSEQQRLA
                     QLADEAKRMRERIQALESILDAEHPNWRDR"
     gene            32455..32814
                     /gene="pspC"
                     /locus_tag="J1Y83_000161"
     CDS             32455..32814
                     /gene="pspC"
                     /locus_tag="J1Y83_000161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response membrane protein PspC"
                     /protein_id="HBA7628352.1"
                     /translation="MAGINLNKKLWRIPQQGMVRGVCAGIANYFDVPVKLVRILVVLS
                     IFFGLALFTLVAYIILSFALDPMPDNMAFGEQLPSSSELLDEVDRELAASETRLREME
                     RYVTSDTFTLRSRFRQL"
     gene            32823..33044
                     /gene="pspD"
                     /locus_tag="J1Y83_000162"
     CDS             32823..33044
                     /gene="pspD"
                     /locus_tag="J1Y83_000162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein PspD"
                     /protein_id="HBA7628353.1"
                     /translation="MNTRWQQAGQKVKPGFKLAGKLVLLTALRYGPAGVAGWAIKSVA
                     RRPLKMLLAVALEPLLSRAANKLAQRYKR"
     gene            33119..33433
                     /gene="pspE"
                     /locus_tag="J1Y83_000163"
     CDS             33119..33433
                     /gene="pspE"
                     /locus_tag="J1Y83_000163"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase PspE"
                     /protein_id="HBA7628354.1"
                     /translation="MFKKGLLALTLVFSLPVFAAEHWIDVRVPEQYQQEHVQGAINIP
                     LKEVKERIATAVPDKNDTVKVYCNAGRQSGQAKEILSEMGYTHVENAGGLKDIAMPKV
                     KG"
     gene            33647..35326
                     /locus_tag="J1Y83_000164"
     CDS             33647..35326
                     /locus_tag="J1Y83_000164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415825.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphorylase"
                     /protein_id="HBA7628355.1"
                     /translation="MEQKITDYLDEIYGGTFTATHLQKLVTRLESAKRLITQRRKKHW
                     DESDVVLITYADQFHSNDLKPLPTFNQFYPQWLQRIFSHAHLLPFYPWSSDDGFSVID
                     YHQVASEAGEWQDIQQLGECSHLMFDFVCNHMSAKSEWFKNYLQQQPGFEDFFIAVDP
                     KTDLSAVTRPRALPLLTPFQMRDHSMRHLWTTFSDDQIDLNYRSPEVLLAMVDVLLCY
                     LEKGAEYVRLDAVGFMWKEPGTNCIHLEKTHLIIKLLRSIIDNVAPGTVIITETNVPH
                     KDNIAYFGEGDDEAHMVYQFSLPPLVLHAVQKQNVEALCAWAQNLTLPSSNTTWFNFL
                     ASHDGIGLNPLRGLLPENEILALVEVLQQEGALVNWKNNPDGTRSPYEMNVTYMDALS
                     RRECSDEERCARFILAHAILLSFPGVPAIYIQSILGSRNDYAGVEKLGYNRAINRKKY
                     SSKEITTELNNKATLSYSVYYKLSHLITLRRSHKEFHPDNNFTIDAINSFVMRIQRSN
                     ADGDCLTGLFNVSENIQHINITDLHGRDLISEVDILGNEITLHPWQVMWIK"
     gene            35340..36632
                     /locus_tag="J1Y83_000165"
     CDS             35340..36632
                     /locus_tag="J1Y83_000165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
                     /protein_id="HBA7628356.1"
                     /translation="MIKSKIVLLSALVSCTLISGCKEENKNNVSIEFMHSSVEQERQA
                     VISKLIARFEKENPGITVKQVPVEEDAYNTKVITLSRSGSLPEVIETSHDYAKVMDKE
                     QLIDRQAVATVISNVGEGAFYDGVLRIVRTEDGSAWTGVPVSAWIGGIWYRKDVLAKA
                     GLEEPKNWQQLLDVAQKLNDPANKKYGIALPTAESVLTEQSFSQFALSNQANVFNAEG
                     KITLDTPEMMQALTYYRNLAANTMPGSNDIMEVKDAFMNGTAPMAIYSTYILPAVIKE
                     GDPKNVGFVVPTEKNSAVYGMLTSLTITAGQKTEETEAAEKFVTFMEQADNIADWVMM
                     SPGAALPVNKAVVTTATWKDNDVIKALGELPNQLISELPNIQVFGAVGDKNFTRMGDV
                     TGSGVVSSMVHNVTVGKADLPGTLQASQKKLDELIEQH"
     gene            36653..37534
                     /locus_tag="J1Y83_000166"
     CDS             36653..37534
                     /locus_tag="J1Y83_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HBA7628357.1"
                     /translation="MNRLFSGRSDMPFALLLLAPSLLLLGGLVAWPMVSNIEISFLRL
                     PLNPNIEATFVGVSNYVRILSDPGFWHSLWMTVWYTALVVAGSTVLGLAVAMFFNREF
                     RLRKTARSLVILSYVTPSISLVFAWKYMFNNGYGIVNYLGVDLLHLYEQAPLWFDNPG
                     SSFVLVVLFAIWRYFPYAFISFLAILQTIDKSLYEAAEMDGANAWQRFRIVTLPAIMP
                     VLATVVTLRTIWMFYMFADVYLLTTKVDILGVYLYKTAFAFNDLGKAAAISVVLFIII
                     FAVILLTRKRVNLNGNK"
     gene            37521..38363
                     /locus_tag="J1Y83_000167"
     CDS             37521..38363
                     /locus_tag="J1Y83_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HBA7628358.1"
                     /translation="MATNKRTLSRIGFYCGLALFLIITLFPFFVMLMTSFKSAKEAIS
                     LHPTLLPQQWTLEHYVDIFNPVIFPFVDYFRNSMVVSVVSSVVAVFLGILGAYALSRL
                     RFKGRMTINASFYTVYMFSGILLVVPLFKIITALGIYDTEMALIITMVTQTLPTAVFM
                     LKSYFDTIPDEIEEAAMMDGLNRLQIIFRITVPLAMSGLISVFVYCFMVAWNDYLFAS
                     IFLSSASNFTLPVGLNALFSTPDYIWGRMMAASLVTALPVVIMYALSERFIKSGLTAG
                     GVKG"
     gene            38394..39446
                     /locus_tag="J1Y83_000168"
     CDS             38394..39446
                     /locus_tag="J1Y83_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase"
                     /protein_id="HBA7628359.1"
                     /translation="MKKLVATAPRVAALVEYEERAISANEVKIRVRFGAPKHGTEVVD
                     FRAASPFIDEEFNGEWQMFTPRLADAPRGIEFGKFQLGNMVVGDIIECGSDVTNYAVG
                     DSVCGYGPLSETVIINAVNNYKLRKMPQGSSWKNAVCYDPAQFAMSGVRDANVRVGDF
                     VVVVGLGAIGQIAIQLAKRAGASVVIGVDPITRRCDIARRHGADFCLNPIGADVGKEI
                     KTLTGKQGADVIIETSGYADALQSALRGLAYGGTISYVAFAKPFAEGFNLGREAHFNN
                     AKIVFSRACSEPNPDYPRWSRKRIEETCWELLMNGYLNCEDLIDPVVTFANSPESYMQ
                     YVDQHPEQSIKMGVTF"
     gene            39465..40253
                     /locus_tag="J1Y83_000169"
     CDS             39465..40253
                     /locus_tag="J1Y83_000169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415830.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HBA7628360.1"
                     /translation="MKIGTQNQAFFPENILEKFRYIKEMGFDGFEIDGKLLVNNLEEV
                     KAAIKETGLPVTTACGGYDGWIGDFIEERRLNGLKQIERILEALAEVGGKGIVVPAAW
                     GMFTFRLPPMTSPRSLDGDRKMVSDSLRVLEQVAARTGTVVYLEPLNRYQDHMINTLA
                     DARRYIVENNLKHVQIIGDFYHMNIEEDNLAQALHDNRDLLGHVHIADNHRYQPGSGT
                     LDFHALFEQLRADNYQGYVVYEGRIRAENPAQAYRDSLAWLRTC"
     gene            40263..41318
                     /locus_tag="J1Y83_000170"
     CDS             40263..41318
                     /locus_tag="J1Y83_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HBA7628361.1"
                     /translation="MKSAMTSSPLRVAIIGAGQVADKVHASYYCTRNDLELVAVCDSR
                     LSQAQALAEKYGNASVWDDPQAMLLAVKPDVVSVCSPNRFHYEHTLMALEAGCHVMCE
                     KPPAMTAEQAREMCDTVRKQGKVLAYDFHHRFALDTQQLREQVTNGVLGEIYVTTARA
                     LRRCGVPGWGVFTNKELQGGGPLIDIGIHMLDAAMYVLGFPAVKSVTAHSFQKIGTQK
                     SCGQFGEWDPATYSVEDSLFGTIEFHNGGILWLETSFALNIREQSIMNVSFCGDKAGA
                     TLFPAHIYTDNNGELMTLMQREMADDNRHLRSMEAFINHVQGKPVMIADAEQGYIIQQ
                     LVAALYQSAETGTRVEL"
     gene            41315..43581
                     /gene="ycjT"
                     /locus_tag="J1Y83_000171"
                     /pseudo
     CDS             41315..43581
                     /gene="ycjT"
                     /locus_tag="J1Y83_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415832.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="kojibiose phosphorylase"
     gene            43578..44237
                     /gene="pgmB"
                     /locus_tag="J1Y83_000172"
     CDS             43578..44237
                     /gene="pgmB"
                     /locus_tag="J1Y83_000172"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HBA7628362.1"
                     /translation="MKLQGVIFDLDGVITDSAHLHFQAWQQIAAEIGISIDAQFNEFL
                     KGISRDESLRRILQHGGKEGDFNPQERAQLAYRKNLLYVHSLRELTVNAVLPGIHNLL
                     VDLRAQQIPVGLASVSLNAPTILAALELREFFTFCADASQLKNSKPDPEIFLAACAGL
                     GVPPQACIGIEDAQAGIEAINASGMRSVGIGAGLTGAQLLLPSTDSLTWPRLSAFWQN
                     V"
     gene            44251..45333
                     /locus_tag="J1Y83_000173"
     CDS             44251..45333
                     /locus_tag="J1Y83_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000057971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBA7628363.1"
                     /translation="MAQLSLQHIQKIYDNQVHVVKDFNLEIADKEFIVFVGPSGCGKS
                     TTLRMIAGLEEISGGDLLIDGKRMNDVPAKARNIAMVFQNYALYPHMTVYDNMAFGLK
                     MQKIAREVIDERVNWAAQILGLREYLKRKPGALSGGQRQRVALGRAIVREAGVFLMDE
                     PLSNLDAKLRVQMRAEISKLHQKLNTTMIYVTHDQTEAMTMATRIVIMKDGIVQQVGA
                     PKTVYNQPANMFVAGFIGSPAMNFIRGTIDGDKFVTETLKLTIPEEKLAVLKTQESLH
                     KPIVMGIRPEDIHPDAQEENNISAKISVAELTGAEFMLYTTVGGHELVVRAGALNDYH
                     AGENITIHFDMTKCHFFDAETEIAIC"
     gene            45378..46283
                     /gene="ompG"
                     /locus_tag="J1Y83_000174"
     CDS             45378..46283
                     /gene="ompG"
                     /locus_tag="J1Y83_000174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monomeric porin OmpG"
                     /protein_id="HBA7628364.1"
                     /translation="MKKLLLCTALVMCAGMACAQAEEKNDWHFNIGAMYEIENVEGYG
                     EDMDGLAEPSVYFNAANGPWRISLAYYQEGPVDYSAGKRGTWFDRPELEVHYQFLESD
                     DFSFGLTGGFRNYGYHYVDEPGKDTANMQRWKIAPDWDVKLTDDLRFNGWLSMYKFAN
                     DLNTTGYADTRVETETSLQYTFNETVALRVNYYLERGFNMDDSRNNGEFSTQEIRAYL
                     PLTLGNHSVTPYTRIGLDRWSNWDWQDDIEREGHDFNRVGLFYGYDFQNGLSVSLEYA
                     FEWQDHDEGDSDKFHYAGVGVNYSF"
     gene            complement(46334..47332)
                     /locus_tag="J1Y83_000175"
     CDS             complement(46334..47332)
                     /locus_tag="J1Y83_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HBA7628365.1"
                     /translation="MSPTIYDIARVAGVSKSTVSRVLNKQTNISPEAREKVLRAIEEL
                     QYQPNKLARALTSSGFDAIMVISTRSTKTTAGNPFFSEVLHAITAKAEEEGFDVILQT
                     SHNPAEDLQKCESKIKQKMIKGIIMLSSPADESFFAQLDKYEIPVVVIGKVEGQYSHV
                     YSVDTDNYGDSIALTDALIESGHKNIACLHAPLDVHVSVDRANGYKQSLATHNIAVRD
                     EWIVDGGYTHETALKAARELLSQSPLPEAVFATDSLKLMSIYRAAAEKNIAIPQQLAV
                     VGYSNETLSFILTPAPGGIDVPTQELGQQSCELLFQLIAGKPSPQNITVATHMSLK"
     gene            47488..48885
                     /locus_tag="J1Y83_000176"
     CDS             47488..48885
                     /locus_tag="J1Y83_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcjX family protein"
                     /protein_id="HBA7628366.1"
                     /translation="MKRLKNELNALVNRGVDRHLRLAVTGLSRSGKTAFITAMVNQLL
                     NIHAGARLPLLSAVREERLLGVKRIPQRDFGIPRFTYDEGLAQLYGDPPAWPTPTRGV
                     SEIRLALRFKSNDSLLRHFKDTSTLYLEIVDYPGEWLLDLPMLAQDYFSWSRQMTGLL
                     NGQRGEWSAKWRMMCEGLDPLAPADENRLADIAAAWTDYLHHCKQQGLHFIQPGRFVL
                     PGDMAGAPALQFFPWPDVDAWGESKLAQADKHTNAGMLRERFNYYCEKVVKGFYKNHF
                     LRFDRQIVLVDCLQPLNSGPQAFNDMRLALTQLMQSFHYGQRTLFRRLFSPVIDKLLF
                     AATKADHVTIDQHANMVSLLQQLIQDAWQNAAFEGISMDCLGLASVQATTSGIIDVNG
                     EKIPALRGNRLSDGAPLTVYPGEVPARLPGQTFWDKQGFQFEAFRPQVMDVDKPLPHI
                     RLDAALEFLIGDKLR"
     gene            48882..49943
                     /locus_tag="J1Y83_000177"
     CDS             48882..49943
                     /locus_tag="J1Y83_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01620 family protein"
                     /protein_id="HBA7628367.1"
                     /translation="MTEPLKPRIDFDGPLEVDQNPKFRAQQTFDENQAQNFAPATLDE
                     AQEEEGQVEAVMDAALRPKRSLWRKMVMGGLALFGASVVGQGVQWTMNAWQTQDWVAL
                     GGCAAGALIIGAGVGSVVTEWRRLWRLRQRAHERDEARDLLHSHGTGKGRAFCEKLAQ
                     QAGIDQSHPALQRWYASIHETQNDREVVSLYAHLVQPVLDAQARREISRSAAESTLMI
                     AVSPLALVDMAFIAWRNLRLINRIATLYGIELGYYSRLRLFKLVLLNIAFAGASELVR
                     EVGMDWMSQDLAARLSTRAAQGIGAGLLTARLGIKAMELCRPLPWIDDDKPRLGDFRR
                     QLIGQVKETLQKGKTPSEK"
     gene            50091..51632
                     /gene="tyrR"
                     /locus_tag="J1Y83_000178"
     CDS             50091..51632
                     /gene="tyrR"
                     /locus_tag="J1Y83_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707228.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator TyrR"
                     /protein_id="HBA7628368.1"
                     /translation="MRLEVFCEDRLGLTRELLDLLVLRGIDLRGIEIDPIGRIYLNFA
                     ELEFESFSSLMAEIRRIAGVTDVRTVPWMPSEREHLALSALLEALPEPVLSVDMKSKV
                     DMANPASCQLFGQKLDRLRNHTAAQLINGFNFLRWLESEPQDSHNEHVVINGQNFLME
                     ITPVYLQDENDQHVLTGAVVMLRSTIRMGRQLQNVAAQDVSAFSQIVAVSPKMKHVVE
                     QAQKLAMLSAPLLITGDTGTGKDLFAYACHQASPRAGKPYLALNCASIPEDAVESELF
                     GHAPEGKKGFFEQANGGSVLLDEIGEMSPRMQAKLLRFLNDGTFRRVGEDHEVHVDVR
                     VICATQKNLVELVQKGVFREDLYYRLNVLTLNLPPLRDCPQDIMPLTELFVARFADEQ
                     GVPRPKLAADLNTVLTRYAWPGNVRQLKNAIYRALTQLDGYELRPQDILLPDYDAATV
                     AVGEDAMEGSLDEITSRFERSVLTQLYRNYPSTRKLAKRLGVSHTAIANKLREYGLSQ
                     KKNEE"
     gene            complement(51676..52182)
                     /gene="tpx"
                     /locus_tag="J1Y83_000179"
     CDS             complement(51676..52182)
                     /gene="tpx"
                     /locus_tag="J1Y83_000179"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol peroxidase"
                     /protein_id="HBA7628369.1"
                     /translation="MSQTVHFQGNPVTVANSIPQAGSKAQTFTLVAKDLSDVTLGQFA
                     GKRKVLNIFPSIDTGVCAASVRKFNQLATEIDNTVVLCISADLPFAQSRFCGAEGLNN
                     VITLSTFRNAEFLQAYGVAIADGPLKGLAARAVVVIDENDNVIFSQLVDEITTEPDYE
                     AALAVLKA"
     gene            52301..53266
                     /gene="ycjG"
                     /locus_tag="J1Y83_000180"
     CDS             52301..53266
                     /gene="ycjG"
                     /locus_tag="J1Y83_000180"
                     /EC_number="5.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707230.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-Ala-D/L-Glu epimerase"
                     /protein_id="HBA7628370.1"
                     /translation="MRTVKVFEEAWPLHTPFVIARGSRSEARVVVVELEEEGIKGTGE
                     CTPYPRYGESDASVMAQIMSVVPQLEKGLTREELQKILPAGAARNALDCALWDLAARK
                     QQQSLAALIGITLPETVTTAQTIVIGTPDQMANSASTLWQAGAKLLKVKLDNHLISER
                     MVAIRTAVPDATLIVDANESWRAEGLAARCQLLADLGVAMLEQPLPAQDDAALENFIH
                     PLPICADESCHTRSNLKALKGRYEIVNIKLDKTGGLTEALALATEARAQGFRLMLGCM
                     LCTSRAISAALPLVPQVSFADLDGPTWLAVDVEPALQFTTGELHL"
     gene            complement(53241..53969)
                     /gene="mpaA"
                     /locus_tag="J1Y83_000181"
     CDS             complement(53241..53969)
                     /gene="mpaA"
                     /locus_tag="J1Y83_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415842.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide amidase MpaA"
                     /protein_id="HBA7628371.1"
                     /translation="MTVTRPRAERGAFPTGTEHYGRSLLGAPLIWFPAPAASHESGLI
                     LAGTHGDENSSIVTLSCALRTLTPSLRRHHVVLCVNPDGCQLGLRANANGVDLNRNFP
                     AANWKEGETVYRWNSAAEERDVVLLTGDKPGSEPETQALCQLIHRIQPAWVVSFHDPL
                     ACIEDPRHSELGEWLAQAFELPLVTSVGYETPGSFGSWCADLNLHCITAEFPPISSDE
                     ASEKYLFAMANLLRWHPKDAIRPS"
     gene            complement(54260..54898)
                     /locus_tag="J1Y83_000182"
     CDS             complement(54260..54898)
                     /locus_tag="J1Y83_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HBA7628372.1"
                     /translation="MKNVLILGAGGQIARHVINQLADKQTIKQTLFARQPAKIHKPYP
                     TNSKIIMGDVLNHAALKQAMQGQDVVYANLTGEDLDIQANSVIAAMKACDVKRLIFVL
                     SLGIYDEVPGKFGEWNNAVIGEPLKPFRRAADAIEASGLEYTILRPAWLTDEDIIDYE
                     LTSRNEPFKGTIVSRKSVAALITDIIDKPEKHIGENIGINQPGTDGDKPFFM"
     gene            complement(54895..54969)
                     /locus_tag="J1Y83_000183"
     CDS             complement(54895..54969)
                     /locus_tag="J1Y83_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA7628373.1"
                     /translation="MKHIQIRNSDMAWHIAANNLGKVS"
     gene            complement(54969..55889)
                     /locus_tag="J1Y83_000184"
     CDS             complement(54969..55889)
                     /locus_tag="J1Y83_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309936.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBA7628374.1"
                     /translation="MMNNKVSFTNSNNPTISLSAVIYFPPKFDETRQYPAIVVSHPGG
                     GVKEQTAGTYAEKLAEKGFVTIAYDASYQGESGGEPRQLENPYIRTEDVSAVIDYLTT
                     LSYVDNTRIGAMGICAGAGYTANAAIQDRRIKAIGTVSAVNIGSMFRNGWENNVKSID
                     ALPYVDAGSNARTSDISSGEYATMPLAPMKESDAPNEELRQAWEYYHTPRAQYPTAPG
                     YATLRSLNQIITYDAYHMAEVYLTQPIQIVAGSQAGSKWMSDDLYDRASSQDKRYHIV
                     EGANHMDLYDGKVYVAEAISVLAPFFEETL"
     gene            56027..56926
                     /locus_tag="J1Y83_000185"
     CDS             56027..56926
                     /locus_tag="J1Y83_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBA7628375.1"
                     /translation="MKREEIADLMAFVVVAEERSFTRAAARLSMAQSALSQIVRRIEE
                     RLGLRLLTRTTRSVVPTEAGEHLLSVLGPMLHDIDSALTSLSDLQNRPSGTIRITTVE
                     HAAKTILLPAMRTFLKSHPEIDIQLTIDYGLTDVVSERFDAGVRLGGEMDKDMIAIRI
                     GPDIPMAIVGSPDYFSRRSAPTSVSQLIDHQAINLYLPTSGTANRWRLIRGGREVRVR
                     MEGQLLLNTIDLIIDAAIDGHGLAYLPYDQVERAIKEKKLIRVLDKFTPDLPGYHLYY
                     PHRRHAGSAFSLFIDRLKYKGAV"
     gene            57263..58876
                     /locus_tag="J1Y83_000186"
     CDS             57263..58876
                     /locus_tag="J1Y83_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415845.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HBA7628376.1"
                     /translation="MKHSVSVTCCALLVSSISLSYAAEVPSGTVLAEKQELVRHIKDE
                     PASLDPAKAVGLPEIQVIRDLFEGLVNQNEKGEIVPGVATQWKSNDNRIWTFTLRDNA
                     KWADGTPVTAQDFVYSWQRLVDPKTLSPFAWFAALAGINNAQAIIDGKAAPDQLGVTA
                     VDAHTLKIQLDKPLPWFVNLTANFAFFPVQKANVESSKEWTKPGKLIGNGAYVLKERV
                     VNEKLVVVPNTHYWDNAKTVLQKVTFLPINQESAATKRYLAGDIDITESFPKNMYQKL
                     LKDIPGQVYTPPQLGTYYYAFNTQKGPTADQRVRLALSMTIDRRLMTEKVLGTGEKPA
                     WHFTPDVTAGFTPEPSPFEQMSQEELNAQAKTLLSAAGYGPQKPLKLTLLYNTSENHQ
                     KIAIAVASMWKKNLGVDVKLQNQEWKTYIDSRNTGNFDVIRASWVGDYNEPSTFLTLL
                     TSTHSGNISRFNNPAYDKVLAQASTENTVKARNADYNTAEKILMEQAPIAPIYQYTNG
                     RLIKPWLKGYPINNPEDVAYSRTMYIVKH"
     gene            complement(58927..59958)
                     /gene="ynaI"
                     /locus_tag="J1Y83_000187"
     CDS             complement(58927..59958)
                     /gene="ynaI"
                     /locus_tag="J1Y83_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low conductance mechanosensitive channel YnaI"
                     /protein_id="HBA7628377.1"
                     /translation="MIAELFTNNALNLVIIFGSCAALILMSFWFRRGNRKRKGFLFHA
                     VQFLIYTIIISAVGSIINYVIENYKLKFITPGVIDFICTSLIAVILTIKLFLLINQFE
                     KQQIKKGRDITSARIMSRIIKITIIVVLVLLYGEHFGMSLSGLLTFGGIGGLAVGMAG
                     KDILSNFFSGIMLYFDRPFSIGDWIRSPDRNIEGTVAEIGWRITKITTFDNRPLYVPN
                     SLFSSISVENPGRMTNRRITTTIGLRYEDAAKVGVIVEAVREMLKNHPAIDQRQTLLV
                     YFNQFADSSLNIMVYCFTKTTVWAEWLAAQQDVYLKIIDIVQSHGADFAFPSQTLYMD
                     NITPPDQGR"
     gene            60202..60459
                     /locus_tag="J1Y83_000188"
     CDS             60202..60459
                     /locus_tag="J1Y83_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2534 family protein"
                     /protein_id="HBA7628378.1"
                     /translation="MIMAKLKSAKGKKFLFGLLAVFIIAASVVTRATIGGVIEQYNIP
                     LSEWTTSMYVIQSSMIFVYSLVFTVLLAIPLGIYFLGGEEQ"
     gene            complement(60509..61459)
                     /gene="uspE"
                     /locus_tag="J1Y83_000189"
     CDS             complement(60509..61459)
                     /gene="uspE"
                     /locus_tag="J1Y83_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspE"
                     /protein_id="HBA7628379.1"
                     /translation="MAMYQNMLVVIDPNQDDQPALRRAVYLHQRIGGKIKVFLPIYDF
                     SYEMTTLLSPDERTAMRQGVISQRTAWIHEQAKYYLNAGVPIEIKVVWHNRPFEAIIQ
                     EVISGGHDLVLKMAHQHDRLEAVIFTPTDWHLLRKCPSPVWMVKDQPWPEGGKALVAV
                     NLASEEPYHNALNEKLVKETIELAEQVNHTEVHLVGAYPVTPINIAIELPEFDPSVYN
                     DAIRGQHLLAMKALRQKFGINENMTHVEKGLPEEVIPDLAEHLQAGIVVLGSVGRTGI
                     SAAFLGNTAEQVIDHLRCDLLVIKPDQYQTPVELDDEEDD"
     gene            complement(61611..62363)
                     /locus_tag="J1Y83_000190"
     CDS             complement(61611..62363)
                     /locus_tag="J1Y83_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007849744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FNR family transcription factor"
                     /protein_id="HBA7628380.1"
                     /translation="MIPEKRIIRRIQSGGCAIHCQDCSISQLCIPFTLNEHELDQLDN
                     IIERKKPIQKGQTLFKAGDELKSLYAIRSGTIKSYTITEQGDEQITGFHLAGDLVGFD
                     AIGSGHHPSFAQALETSMVCEIPFETLDDLSGKMPNLRQQMMRLMSGEIKGDQDMILL
                     LSKKNAEERLAAFIYNLSRRFAQRGFSPREFRLTMTRGDIGNYLGLTVETISRLLGRF
                     QKSGMLAVKGKYITIENNDALAQLAGHTRNVA"
BASE COUNT        15583 a        14980 c        16344 g        15553 t
ORIGIN      
        1 ggtaaattac ctgtaaagta gtgtctaatt acacacctag caaactccag ttttcaaata
       61 tctatttcag gtcaaaagat tgaattattc actggagacg attcgtaaat tcgctggatt
      121 tttgactaaa gtttctttag gatttgtctc atcgtatgat taattacacc aatcgttcaa
      181 cgtcttgctg ataagtgatg gcgattatat gaggttagca atgccttctg gaaatcagga
      241 accccgccgc aatcctgaat taaaacgtaa agcctggctg gcggtttttc ttggttctgc
      301 acttttctgg gtggttgtcg cactgcttat ttggaaattg tggggataac tatggtcggt
      361 caggagcaac tggagtcgtc accattatgt cagcatagtg acaatgaacc agaagcaaaa
      421 cgggaatgtt ccgtcgttat tccagacgac tggcaactaa catcgcagca acaagccttt
      481 atagaactgt ttgctgaaga tgatcagccg aaacaataat tatcatcatt cttattaccc
      541 atttttaatg aattacaggg cttttaatac accgcagcaa taacaggttg agttatctca
      601 acacaaagta ataaccgtta agggtgtagc ctatgatcaa cacaaatatg aaatattggt
      661 cctggatggg cgcattttct ctgtcgatgc tcttctgggc cgaactcctc tggatcatta
      721 ctcactgatc cctgaccccg ctgtttgctt tgcctcaagg tttctcctct tttatcaatt
      781 tgggttgtta tcaaatcgtt acgcgatgtt tgtgttatct ttaatattca ccctgaagag
      841 aatcagggct tcgcaaccct gtcattaagg aggagctatg tcgtcaaccc tacgagaagc
      901 cagtaaagac acgttgcagg ccaaagataa aacttaccac tactacagcc tgccgcttgc
      961 tgccaaatca ctgggcgata tcacccgtct acccaagtca ctcaaagttt tgctcgaaaa
     1021 cctgctgcgt tggcaggatg gtaaatcggt taacgaagag gatatccacg cgctggcggg
     1081 atggctgaaa aatgcccatg ctgaccgtga aattgcctac cgcccggcaa gagtgctgat
     1141 gcaggacttt accggcgtac ctgccgttgt tgatctggcg gcaatgcgcg aagcggttaa
     1201 acgcctcggc ggcgatactg caaaggttaa cccactctca ccggtcgacc tggtcattga
     1261 ccactcggtg accgtcgatc gttttggtga tgatgaggca tttgaagaaa acgtacgcct
     1321 ggaaatggag cgcaaccacg aacgttatgt gttcctgaaa tggggaaagc aggcgttcag
     1381 tcggtttagc gtcgtgccgc caggcacagg catttgccat caggttaacc tcgaatatct
     1441 cggcaaagca gtgtggagcg aattgcagga cggtgaatgg attgcttatc cggatacact
     1501 cgttggtact gactcgcaca ccaccatgat caacggcctt ggcgtgctgg ggtggggcgt
     1561 tggtggcatc gaagcagaag ccgcaatgtt aggccagccg gtttccatgc ttatcccgga
     1621 cgtagtgggc tttaaactta ccggaaaatt acgcgaaggt attaccgcca cagacctggt
     1681 tctcactgtt acccaaatgt tgcgcaaaca tggcgtggtg gggaaattcg tcgaatttta
     1741 tggtgatggg ctggattcac taccgctggc ggatcgcgcc actattgcca atatgtcacc
     1801 agaatatggt gccacctgtg gcttcttccc aatcgatgct gtcaccctcg attacatgcg
     1861 tttaagcggg cgcagtgaag atcaggtcga gttggtcgaa aaatatgcca aagcgcaggg
     1921 catgtggcgt aatccgggcg atgaaccaat ttttaccagt gtgttagaac tggatatgaa
     1981 tgacgttgaa gcgagcctgg cagggccaaa acgcccgcag gatcgcgttg cattgcccga
     2041 tgtaccaaaa gcatttgctg ccagtagcga actggaagtg aatgccacgc ataaagatcg
     2101 cctgccggtc gattatgtta tgaacggaca tcagtatcag ttacctgatg gcgctgtggt
     2161 cattgctgcg ataacatcgt gcaccaacac ctctaaccca agtgtgttga tggccgcagg
     2221 cttgctggcg aaaaaagccg taactctggg cctcaagcgg caaccgtggg tcaaagcgtc
     2281 gctggcaccg ggttcgaaag tcgtttctga ttatctggca aaagcgaaac tgacaccgta
     2341 tctcgatgaa ctgggattta accttgtggg atacggttgt accacctgta ttggtaactc
     2401 tggaccgttg cccgatccta tcgaaacggc aatcaaaaaa ggcgatttaa ccgtcggtgc
     2461 agtgctgtcc ggcaaccgta actttgaagg ccgtatccat ccgctggtta aaaccaactg
     2521 gctggcctcg ccgccgctgg tggttgccta tgcgctggcg ggaaatatga atatcaacct
     2581 ggcttctgag cctatcggcc atgatcgcaa aggcgagccg gtatatctga aagatatctg
     2641 gccatcggca caaggaattg cccgtgcggt agaccaagtc tccacagaaa tgttccgcaa
     2701 agagtacgca gaagtttttg aaggcacagc tgagtggaag gaaattaacg tcacacgatc
     2761 cgatacctac ggttggcagg aggattcaac ctatattcgc ttatcgcctt tctttgatga
     2821 aatgcaggca acaccagcac ctgtggaaga tattcacggt gcgcggatcc tcgcaatgct
     2881 gggggattca gtcaccactg accatatctc tccggcgggc agtattaagc ccgacagccc
     2941 ggcgggtcga tatctacaag gtcggggtgt tgagcggaaa gactttaact cctatggttc
     3001 gcggcgtggt aaccatgaag tgatgatgcg cggcaccttc gccaatattc gcatccgtaa
     3061 tgaaatggtg cctggcgttg aaggggggat gacgtgccat ttacctgaca gcgatgtcgt
     3121 ctctatttat gatgccgcaa tgcgctataa gcaggagcaa acgccgctgg cggtgattgc
     3181 cgggaaagag tatggatcag gctccagccg tgactgggcg gcaaaaggtc cgcgtctgct
     3241 tggtattcgt gttgtgattg ccgaatcgtt tgaacgaatt caccgttcga atttaattgg
     3301 catgggcatc ctgccgctgg aatttccaca aggtgtgacg cgtaaaacgt tagagcttac
     3361 cggggaagag aagattgata ttggtgatct gcaaaatcta caacccggcg cgacggttcc
     3421 ggtgacgctt acgcgcgcgg atggtagcca ggaagtcgta ccctgccgtt gtcgtatcga
     3481 caccgcgacg gagttgacct actaccagaa cgacggcatt ttgcattatg tcattcgtaa
     3541 tatgttgaag taacgagtat ttgcttgccg gttattttgc ttccggcaag caaattaatt
     3601 acacaatgca agagggttat ttgttcagca aatgacccat tttctcggct ttggtatcga
     3661 gataatgttc gttattgggg ttacgaccta caatcaacgg tacgcgttca ataatattaa
     3721 tccctgcttc ggtcagaatt tcgacttttt tcgggttatt ggttaacaag cggacttcat
     3781 tgacgccaag gagtttgaac atatcagcgc aaagagtgaa gtcgcgctca tcagcggcga
     3841 agcctaactg gtggttagcc tctacagtat cgtaaccttg atcctgcagt gcgtaagcgc
     3901 ggattttatt cagcagacca atgttacgac cttcctgacg gtgatacagc aaaataccac
     3961 ggccttcctc ggcaatttgc gtcaatgccg cttcgagctg gaagccacaa tcgcagcgca
     4021 agctgaacag ggcgtcaccg gtcagacatt cggaatggac gcgcgcaagt accggggtat
     4081 gcccggaaat atcgccatag actagcgcga catgatcgtg tccggttgcc agttcttcaa
     4141 atcccaccat caggaaatcg ccccatgggg ttggcagttt ggcttctgcc acacgtttaa
     4201 gctgcatgaa attctccaga taatgctggt tctgtattgg cttattttgc cataacgaga
     4261 aggggttcac ctaatcacga cgcgtcgatc gttcacggaa tggcacaaat ctgtcaattt
     4321 ttcctggaac tggcattttc agttatgatt gtgggactta tcaaaaagga gaggccatgc
     4381 gttcgattgc cagacgtacc gcagtgggag ctgcactatt gcttgtcatg ccagtagccg
     4441 tatggctttc tggctggcgt tggcaacctg gagaacaaag ttggttacta aaagcggctt
     4501 tttgggttac tgaaactgtc acccagccct ggggcgtcat tacacatttg attttattcg
     4561 gctggtttct ctggtgtctg cgttttcgca ttaaggctgc cattatgtta tttgccattc
     4621 tggcggcggc aatccttatg ggacaaggcg ttaaatcctg gatcaaagac aaagtccagg
     4681 aaccacgacc ttttgttatc tggctggaaa aaacacatca tattccggtt gatgagttct
     4741 acactttaaa gcgagcagaa cgcggaaatc tggtgaaaga acagttggct gaagagaaaa
     4801 atatcccaca atatttgcgt tcacactggc agaaagagac ggggtttgcc tttccttccg
     4861 gtcacacgat gtttgctgcc agttgggcac tgctggccgt tggtttgctg tggccgcgtc
     4921 ggcgaacgtt aaccattgct atcttgttgg tctgggcaac gggagtcatg ggaagccgcc
     4981 tgctgctcgg gatgcattgg ccacgcgatc tggttgtagc tacgttgatt tcgtgggcgc
     5041 tggtggcggt ggcaacgtgg cttgcgcaac gaatttgtgg gccattaaca ccacctgcgg
     5101 aagaaaatcg cgaaattgca caacgagaac aagaaagtta acgctggttg attttccgaa
     5161 tttagccctt aaatcatcaa caatgcgcgt ggatgccatt tcgcagacgg cgcgaaaatg
     5221 gtactttaaa gggctattgc ggtaagttga ccataattta ttcgccctaa ccacataacg
     5281 ggaagtaatg tgaaatattt actcattttc ttactagtgt tagcgatctt cgtgatttcg
     5341 gtcacgttgg gtgcgcagaa cgatcaacag gtgacgttta attatctgtt agcgcaaggg
     5401 gagtaccgta tttccacatt gctggcggta ttgtttgctg cggggtttgc tatcggttgg
     5461 ttgatttgtg gcctgttctg gctgcgagtt cgtgtttccc tggcgcgcgc tgaacgtaaa
     5521 ataaagcgac tggaaaacca gctttcacct gcgactgacg tggctgtagc gccgcactcg
     5581 tctgcgtcga aggaataact ttctatgctg gagttgttgt ttctgctttt gcctgtagcc
     5641 gctgcctatg gctggtatat gggccgcaga agtgcgcaac aaaacaagca agatgaagcc
     5701 aaccgcttgt cgcgtgatta cgtagcgggg gttaacttcc tgcttagtaa tcaacaggat
     5761 aaagcggtag acctgtttct cgatatgctt aaagaggata caggtaccgt tgaagcccac
     5821 cttacgctcg gaaacctgtt ccgttcgcgt ggcgaagttg atcgcgctat tcgcatccat
     5881 cagaccctaa tggaaagcgc ctcgctgacc tatgaacagc ggcttttggc gattcaacaa
     5941 ctggggcgtg attacatggc tgccgggtta tacgaccgcg cggaagacat gttcaatcag
     6001 ctgaccgatg aaactgactt ccgcattggc gcgctgcaac aactgctaca aatttaccag
     6061 gctaccagcg agtggcagaa agcaattgat gttgccgaac gcctggtgaa gctgggtaaa
     6121 gataaacagc gcgtcgaaat tgcccatttc tactgtgagt tagctctgca gcatatggcc
     6181 agcgacgatc tcgatcgtgc catgactttg ctaaaaaaag gtgcggcggc agataaaaac
     6241 agcgcccgcg tatccatcat gatgggacgc gtgtttatgg cgaaaggaga atacgccaaa
     6301 gccgtcgaaa gtttgcaacg tgtcatatcc caggacagag aactggtcag cgaaacgctg
     6361 gaaatgctgc aaacatgcta tcagcagttg ggtaaaactg ccgaatgggc agaattcctg
     6421 cagcgcgcgg tggaagagaa caccggtgcc gatgctgaat tgatgcttgc ggatatcatc
     6481 gaagcgcgcg acggtagtga ggccgcacag gtctatatta cacgccagct tcagcgtcat
     6541 ccgaccatgc gtgtgttcca taagttaatg gattaccact taaatgaagc agaagaaggg
     6601 cgtgccaaag agagtctgat ggtgctgcgt gacatggttg gcgagaaggt acgtagtaag
     6661 ccccgttatc gctgccagaa atgtggtttt accgcataca ccctctactg gcattgtccg
     6721 tcttgtcggg cctggtcaac cattaaacca attcgcggtc ttgatggcct gtaattttaa
     6781 aaaaatccga ctttagttac aacatactaa ttattgatgt tctattgtgc tccggcaccg
     6841 acggcgcgga aaagcctgcc agaggagaaa tcgcaactgt taatttttta tttccacggg
     6901 tagaatgctc gccgtttacc tgtttcgcgc cacttccggt gcccatcatc aagaaggtct
     6961 ggtcatgacg ttaactgctt catcttcttc ccgcgctgtt acgaattctc ctgtagttgt
     7021 tgcccttgat tatcataatc gcgatgccgc gatggccttt gtcgacaaga tcgacccacg
     7081 cgattgtcgt ctgaaggtcg gcaaagagat gtttacattg tttgggccac agtttgtgag
     7141 cgaacttcaa cagcgtggtt ttgatatctt tcttgacctg aaattccacg atattcccaa
     7201 cactgcagca cacgctgtcg ctgctgcggc tgacttaggc gtgtggatgg tgaatgttca
     7261 tgcctccggt ggggcgcgta tgatggccgc agcgcgtgag gcactggttc cgtttggcaa
     7321 agatgcaccg cttttgattg ctgtgacagt gttgaccagc atggaagcca gcgacctggc
     7381 cgatcttggc gtgacactgt cacctgcaga ttatgcagaa cgtctggcgg cactgacgca
     7441 aaaatgtggc cttgatggtg tggtgtgttc tgctcaggaa gctgtgcgct ttaaacaggt
     7501 attcggtcag gagttcaaac tggttacgcc gggcattcgt ccgcagggga gtgatgctgg
     7561 tgaccagcgc cgcattatga cgccagaaca ggcgttgtcg gctggtgttg attatatggt
     7621 gattggtcgc ccggtaacgc aatcggtaga tccagcgcag acgctgaaag cgatcaacgc
     7681 ctctttacag cggagtgcat gatgagtgat tccaacagtc gtctggtcta ctcaacggaa
     7741 accggacgta ttgatgagcc aaaagcggcc cctgtacgcc ctaaaggcga cggtgtggtg
     7801 cgtattcagc gtcagaccag tggacgcaaa ggtaagggcg tttgcctgat taccggtgtc
     7861 gatctcgatg atgccgaact gacgaaactg gcagctgaac tgaagaaaaa atgcggctgc
     7921 ggcggagcag ttaaagatgg aattattgaa attcagggcg ataagcgtga tttattaaaa
     7981 tcaatgcttg aagcgaaagg gatgaaggta aaactcgcag gcggctaaca taaaaagcca
     8041 cggatatatc cgtggctttc gaatatttta ctgtgtgtat tattcagtta tttcgtactg
     8101 aacagaatca agcgtattat aattgcgagt cgataatcga catttattta cgattattta
     8161 ccgacctggt gaccaataac accaccgacg gctgcaccac ctaatgtacc caacgtactg
     8221 ccatcggtca gtactgcacc gcctaatgcc cctgcacctg caccgattgc ggtgttgcgg
     8281 tcccgtttag accagttaga acaggcactc agagacattg ccagggtaat tgccagaaca
     8341 gccgcggtca tttttttgct cgttacaaac ataatactct ctcctgaatt tatgattcac
     8401 ggaagtaagc tctctataac tataatagct aagaataagc caggtgaaat tattccgtaa
     8461 aatccgctcg cgcgcagggt tatatcacgc aggtgataat gacttcctgt tatatcgctg
     8521 ataataattt tatatcttga gagtgttaat aacaggtaaa tagtcttaat tatcaaccag
     8581 gaatcatctt agagcggatg atttgccaaa ctgcaaatca tccgtagaga agggaaatgg
     8641 ttaaatgtca acgacgtgaa cggtgagttt actgtgctca agatgcatca gatcgctggc
     8701 gcgtattttc gaatcggtaa tcacgcgatt gaatcgctca acggggccga tggagtatga
     8761 atgtacagca ccaaatttcg agctgtcggt caggactatc gcctcgcact ctttttccag
     8821 cacggcattg accacatcgg tacgcatcat atcgcgaccg gtaaacccag tttcaggttg
     8881 ccagccatca ataccaataa atgctttgct gaaatgcacc tgttggatgc actggcgtgt
     8941 caaagggcca accatacttt cgcttttttt ctggtacacg ccaccgagca aaatgacttc
     9001 acaaggcgcg tctttcagca aatgcgcgat gtagctgctg accgtgatga tagtggcatt
     9061 tttcttctgc tcgcccagag tcctggcgag cagggcattg ctgctaccat tttcgataaa
     9121 gatggtttcg cccggttgaa ccagtgacgc ggcaaactcg gcgagttcac gcttcagcgt
     9181 atagttgctc atcatacggg tttcgacgtc atcactatca agcgaaactg caaaaccatg
     9241 tgcacgtcgg aggtaactca gtttttcgag ggtgttgaga tcctggcgaa tggtaacttc
     9301 agaaactcca gtggcttttg ccagatcggt tacgctaacc tgaccctggt caatgaccat
     9361 ctgtagaata gtttgttgtc gggaattcat cgctgtaatt cttataacgt tataatacta
     9421 aataaaaaaa tactgacgcg ccgggcatcc tcaaatagga aaacctgaag gcacgattgt
     9481 tagatctccc acggtgcctt cgggatgtct ggcgtagatt cagcaacctg gcgcaagagt
     9541 tctgctttaa gtatctcgag attatggatc gcagagtggt aatcgcctgc cacaaggata
     9601 tctaacacaa tatcaatacg ttcggcaacg cgctgggcat caaattcaga catcaaggac
     9661 atccttatag caaaagggga aaaatgaata atgcaaaaat tgccgctaaa agagaagtgt
     9721 ttaacagcaa cggctaatta tcatccggga atacgatata taatgacgga atatagcgct
     9781 aagtatatat attcatctac ttatgcgcgc ttcagatagc gtttatacca gcgttcgaag
     9841 gcaacggcgg gcatcggttt ggcaaacaaa aatccttgtc gctcattgat cccgttcttg
     9901 gttaaaaaag catcttcctt tgcactttct acaccttcgg cgatcacctg aagattcaat
     9961 gcctgggcca cagcgacgat cgcccggacc agtgattgcg agacaggctg tttgtgaata
    10021 tcccgaacaa aaacctggtc aagtttgatg gcatcgatcg gaaagcgcgc cagttgcgaa
    10081 agtgaagagt agccggtacc aaaatcgtcc agatgaactt gcgcacctaa tcggctaaat
    10141 tgttgaataa cagacagtgc cagttcatca ttctcaatca ggcagctctc cgtcagttca
    10201 acatctatag ggcagtattc aaaattgagt tcctggagaa cctgtttcag ggcggtgaaa
    10261 atggtttgat cggcgagctg acgtgcagaa atatttaccg ccactcgcag gttaatgcct
    10321 ttatcccgcc acttcgctac ctggcgtacg acatcgagaa tcacccaacg gcctaaaggc
    10381 acaattagcc ctgattcttc ggcgtaggaa atgaagtcca acggtggaat caacccacgt
    10441 tcaggtgact gccaacgtac tagtgcttcc agactgcgca cttcgccacg ccaggtgatt
    10501 ttcggttgat agtgaataac caactgatcg ttttccagtg ctttacgcaa gttggtatcc
    10561 agccagagat attcaaatac ccgctgattc atttctgggg taaaaacgca aaattgtcct
    10621 cgtccgcctt ccttcgctgt gtacattgct gtgtcggcgt gacgaataat agccgcgctg
    10681 tccgaaccat gttcgggaga gagtgaaata cctactgaac agctggtata aacttcaatt
    10741 aaaccaatgc gaaaggggag tcgtaagcgg gtcaaaattc gtgatgccaa tgcttccagc
    10801 gcgctttgtg aggtattgga tgccagtacc agaaactcat cgccacctgg acgcgccaat
    10861 acctggtcat gttcgagaca gcttaaaata gccaatgaca cgtcgcgtaa taactggtca
    10921 ccaaacaaat gcccataggc gtcgttgacc tttttaaaat tatccaaatc aagatacaca
    10981 accccaactt tattgttatc tgcctgatta atagcgtgat cgattaactc ctgcatcgcg
    11041 ttacgattcg gcagtccggt gatactgtcg gtatttgcca gaatacgcag tcgctcctga
    11101 gcgcggcgct cttcggtaat gtcggtgccg gaacagatta aaaaaatctc gtttttgcca
    11161 ctgccgctgt ggacaaattt attgcgaaac agaaacagcc gctggccttt acgtgttggt
    11221 atccatagtt cgacttcata tgcattgccg cttcgaaaaa atacacggtt attgcgcctg
    11281 gatgccgcag cttcacgacg actcataaac agtttaaaca cgctttgccc aatgacgtcg
    11341 tgttctttca accctgtgta atcttcacat aaccgattga agcgctggat attcccccgg
    11401 ctgtcgagaa tcactattgc ggaatgtgct tcagaaacta cctgctcagc aaaggaaagc
    11461 ccatgtgata aatcacgagc aaccgccggt gtgtcatgcc atgcagacgc gttgcctccc
    11521 cattctcgtt tattgatttt tcgtcctacc agatgcacag aaagatcgct ttcatcgacg
    11581 gtcagactca tcatcaggct ggaggtgatg accgtcattt ccctgatgcg agcagcctgt
    11641 tcggcagata gttgcaaaat acgatcgggt tctgtgattt cggtggtaga aaagcgcaaa
    11701 acatcgctgc tttccgtcaa ccgccagtat ggattgtgcg atccgagaaa gttgtacaac
    11761 gttgcggact ccctaacggt tttcatgatg catatcctcc ggttaacagc ggagtccgca
    11821 gttaaataaa aggaacaaca tgacttacat gaaattaacg gcggctaaac acattgtttt
    11881 tttatatatt tatttgtaat ccagttttgg aaaaacgcca gttttcaaac gaaagtcagt
    11941 taaaaatctg ccgggatatg aggaaggttg agcggggaaa taaacggccc atccatgagg
    12001 aatgggccgt gaaaggagat tacgcgactg ggcgcgcaat aatgctgcgg gtttccatgc
    12061 ggacttcggc aatggtgacg tcaataacgt cagtcacttt gtaagccgtt tcacctttaa
    12121 tttgtacagt gccgttttcc tggctgcaaa ccagttcatc gcgcacagcg tgtaagaaag
    12181 gtgctggaat aaaggcgata gcgccgttat caaccaaacg aacacgcatg ccgccacggc
    12241 tgatatcgac aatttccgcc gcgaaacggg tgtcggtccc ggctttgtct ttcaggaagc
    12301 gtgcgtataa ccagtcgcca acatcacgtt cagccatccg gttgagacgg cgacgctcgg
    12361 ccatttggac agtgatttca tcctgtggac gcgtcgcagt ttcgcctttg ataaccgctt
    12421 tcagcagacg gtggttgatc atgtcgccat atttacggat cggcgaagtc caggtggcgt
    12481 atgcttccag accgaggcca aagtgaggac cgggttcagt gctaatttca gcaaatgact
    12541 ggaagcgacg aatgcggctg tcgaggaaac cagttggttg cgcatccagt tcacgacgca
    12601 gtttgcagaa accgtccagc gtgagcactt cttcagcatc gacatgcaga ccgtgcgttt
    12661 tcagcaacgc tgccaacgcg tcggcattcg ccggatcaaa gcccatatgc acgttataga
    12721 tgccaaaacc gagcttatcg cgcagtacgc gggccgcaca aatgttagcg gcaatcatcg
    12781 cttcttcgac gatacggttg gcaatgcgac gaggctcggc gacgatatcc agcacttcac
    12841 ctttttcacc gagaataaag cggtaatccg ggcgatcttt aaacaccagt gcgtggttat
    12901 gacgccactc gccgcggcgt tggcaaattt gtgctagcaa acggacttgt tcggcaattg
    12961 cttcgctttc aggctgccag tcaccggtat tttccagcca gtcagaaacc tggtcataca
    13021 ccagcttcgc tttggattcg atggtggcgg caaagaattc gatattatct tcaatggtgc
    13081 catcagtgga gagcgtcatg cggcatgcca gtaccgggcg gacttcattg gcgcgcagtg
    13141 agcagagatc gtcagaaagc tcgcgaggca gcatagggat gttgaagcca ggcagatagt
    13201 tggtgaatgc gcgaattttc gcggctttgt ccagcttgct gccttcagca atccacgcgg
    13261 ttggatcggc aatcgccaca atcagctgaa gtttgccatc cggtaacgcc ttggcgaaaa
    13321 gggcgtcatc catatcttct gtgctggcac tgtcgatggt gacaaaatcc agcgcagtca
    13381 gatcttcacg aaccagacct tcatcgagca tttcggtagc gacgccgtct ggtgcttctt
    13441 tttccagatt atgacgcgca agggtaaccc accacggtac aaagtgatcg tcaccaaaag
    13501 tgatgtattg tgtcagttca gcatagaaag aacgatcgcc tttcagcgga tgacggcgca
    13561 tttcggcaac cgcccagtcg ccttctttaa actcgtggtt caggccacgg gctgcgcggc
    13621 aaggaatggc gtcttttaag agtggatgat caggaacgat ggccagacgg tcatttttgc
    13681 cctgaacttt acccacgaaa cgagtcagga acggttcaac cagttcttct ggctctgcgg
    13741 attcacgttc tttttcactg tggatcaccg cgataattcg gtcgccatgc atgacttttt
    13801 tcatctgcgg cggcggaatg aaataacttt tttgcgcgtc gacttccagg aagccaaagc
    13861 ctttttctgt ggcttttacc accccttcag cgcgtggcgt ctgggaatgc agttgctgtt
    13921 taagctgcgc tagcagcggg ttgtcctgaa acataattgt ctattttggt ggccattaga
    13981 gcggctgaca gttttacgcg aatctgtctg acgcggcaag gttaatatgt ctcacccaac
    14041 gcgattttta agcgatttat ccagccacac agcccgctcc ataccagcag attaataatc
    14101 tgcgttgatg atttttcgtg ttcgagtaat gggctgaact gggcggcgct aaagcgatcg
    14161 ggtgctcggg tcagccattg tatcgcctga acggtagcgc gctcaacaga atgacggtca
    14221 gcccatcggt taatttcatt ttcgcccttg cgcagaagga ggctgatttt tttatattca
    14281 tttgatgaat tcatacaggc ggcaaaacaa gcaggactgc catttatccg cgcgcataac
    14341 aacgccgcaa gcaggttacc cggctgtgtt ttaacccgag tgctgtttaa caacatttcg
    14401 agaagggaaa gtgaaatagg gtggagagag agtgtttcgg taagttggca aagttctgca
    14461 gtcgaacttt ttgtgtactc ttcaggaagt ttttccacct catagctgct tcgccagcgt
    14521 aatgattcat cagcaaacag tgacgcgtcg gcgtagtttt gtatctccag cccgggtaac
    14581 cagcgtactg gatgcccgag ataagcctga aatgtcgcaa ttgtccgcgc ctgaaagcca
    14641 ataaaaccaa tgatttggtt aatgatcaca cagtcacagg cagtaagacc aacgtcattc
    14701 agatgctggc gcgatcggtt gtcgataata gaaggcgagc tagccagttg gcgggcatat
    14761 tgcgtgattt gtgccaaccg atgattactt tccctggagg aatcaggccc ggggagcggc
    14821 gtaagtcgcg ccgaatagta gttacataac cgttggacgc cgcaaacctg agcaaccgtc
    14881 agggccgtcg acaagcgttc ataagcggtg aaggtctgtg aacgggtcac ggtaagctgg
    14941 tcagggaaca attctactgc aagaaggcgc gcagggacca gccatgaaag gaagggtgcc
    15001 agcgtttctt catcaaggat cacgtcgagc agaaagggat cgctgacctt tgcggcttcc
    15061 gggaccagag gcagaacgtc atactccgca gtactggatt gcgtttcatg ataccagtgg
    15121 cttttgccgg tgatgtggcg ttgttccatg ggcgctcctt ggtcgtaaag gaaatcgtta
    15181 tcctgacgca aggcgagaag gggagaaaga cggatcggcg ataacaaata tcagaaaggt
    15241 ataacagaga taacgggcgg cagaacgccg cccaatattt accaacagaa cgattatttc
    15301 agttcgagtt cgttcattgc agcgatgctg aaaccgccgt caacgtggac cacttcaccg
    15361 gagataccgg cagagagatc ggagcacagg aatgccgcgg agttacccac atcttcaata
    15421 gtaacggtac ggcgaatcgg ggtaacggct tcgcaatgag ccagcatttt gcggaagtct
    15481 ttgataccgg aagccgccag agtacggatc ggacccgcag agatggcgtt aacacgcaca
    15541 ccttccggac ccatcgcgtt cgccatatag cgcacgttcg cttccagaga cgcttttgcc
    15601 agacccataa cgttgtaatt cgggatagcg cgctcagcgc caaggtagga aagggtcagc
    15661 agggcagaac ccggattcag catggagcgg caagcttttg ccattgcaac gaagctgtag
    15721 gagctgatgt cgtgggcaat tttgaagcct tcacgagtaa cggcgttaac atagtcacca
    15781 tccagctgat cgccaggtgc aaaaccaata gagtgtacga aaccgtcaaa tttcggccaa
    15841 actttcccca gctcagcgaa catggtgtcg atgctggtat cttcggcaac atcgcactgc
    15901 agaacgatgt cagaacccaa ttgagcggca aattcttcta cgcggccttt cagtttgtcg
    15961 ttctggtagg tgaatgccag ttcagctcct tcgcggtgca tcgcctgagc gataccgtag
    16021 gcgatggaca gtttgctggc aacaccggtt accagaatgc gcttaccgga aagaaaaccc
    16081 atagctttaa tccttattgt tgatgcttgt tgtgcctgaa aatcaggcga ttcgtcgttt
    16141 tagtaaacag tacgaacaga taaacggtta ttataatcaa cctggctgtg ggtggctata
    16201 gttgcgcttt ctgtagcgga gagaagctgc ggtacagagc attttgcgtt atctactcct
    16261 gacgcctgca agggggaggt tggggaaata tcttatagaa tctgactttg aaactattcg
    16321 tattagctcc tataagtttg atgatgaaaa cagaactggc gacaacaagt cgctgtcttt
    16381 ctgatgaatt gaccccaact taatattgtc aactatcttc acagaatctc cacaatctct
    16441 tcattatcag aatatgcatc atgaataaga taaaaatact ttccattact ttggagaaac
    16501 gtataatcag caaaaatgag aacgatcatt ctcaccaagt gataggccag gagacagaaa
    16561 tgaccagcaa gctggagata cgccacaaac agcgtcagga tgaaatcatt aacgccgccc
    16621 gtcagtgttt tcgtcgctgc ggatttcatg ccgccagtat gtcgcaaatt gccagcgaag
    16681 ctcagcttag cgtgggtcag atatatcgtt acttcgccaa caaagacgcc attattgaag
    16741 agatggttcg ccgtatcatc gactttcgta ttgcccaaat ggacattgac gcgcgtaccg
    16801 atcacttccc ggaagtcctt gccttgcgta aatccttaaa tgaggacgac gacgcgctga
    16861 tgctggaagt ggctgcagaa gccacacgca acccgcgggt tatggcaatg cttgaagaag
    16921 ctgacgccag aatgttcgct aacggttgtg cacatatgaa aagaatgcat ccccatctta
    16981 gcgacgagca cattcgttgc tgcgttgaag tgtttgctac catgatggaa ggtacggttt
    17041 atcgtcgttt aacaccgcaa aaaagcgatc cacaacatct gcaggaaata tatcaggata
    17101 ttgttagtat gttgataaat aaataagaac agaattaacc aatagcgggg gacattaatc
    17161 ccccttattt cattgcgaaa ttcatttcgc gatgcgggtt cgttataact gaatatccct
    17221 gacatatcct cattaatttc aatgaggagg gtatttctca ccctggagaa aataatgaag
    17281 tatatagcaa catctgtagt ggcaatgctg ctcttatcgg gttgtgataa tacgcaaagt
    17341 aacaattcat ccccgtcaga aacagaagtc ggcgttgtta cgctcaaatc tcaaccggtt
    17401 tcggtagtca gtgaattaac cggacgcacc agtgctgcgc tcagtgctga agtacgtccg
    17461 caggttgggg gaattatcca gaaacgctta tttaaggaag gtgatctggt caaggctgga
    17521 cagcctctct accagattga tgcggccagt tatcaggctg catggaatga agcccgggca
    17581 gcattacaac aagcacaggc actggtaaaa gccgattgcc agaaagcgca gcgttatgcc
    17641 cgactggtga aagagaacgg tgtttcacaa caggatgctg atgatgctca gtctacctgt
    17701 gcacaagata aagccagtgt agaggcgaaa aaagccgcac tggaaactgc gcgcattaat
    17761 cttgactgga ccacggtaac cgcaccgatt tcggggcgta ttggcatttc gtcggtaacc
    17821 cctggcgcac tggtgaccgc gtcgcaggat acagcgttaa cgactattcg tggtctggat
    17881 acaatgtatg tcgacctcac tcgctccagt gtcgatttat tacgtctgcg taaacagtca
    17941 ctggcgacca acagtgacac catgagcgtc tcacttattc tggaagatgg cacaacctac
    18001 agcgaaaaag ggcgtctgga actcaccgaa gtcgcggtgg atgagtctac cggttcggtg
    18061 acattacggg caattttccc caatccacaa cagcagttat taccgggaat gtttgttcgc
    18121 gctcgtgtcg atgaaggcgt gatggaagac gctattctcg cgcctcaaca gggcgttacg
    18181 cgcgatgcta aaggcaatgc aactgcgctg gtggtgaata aagacaataa agtagagcag
    18241 cgaacgctcg aaacgggaga aacgtatggt gataaatggc tggtgctgaa cggcctgcac
    18301 aacggcgacc gactgattgt tgaaggttct gccaaagtca cttcagggca gaccgtcaag
    18361 gctgttgaag ttcaggctaa tggaggcaac gcctgatgtt ttcgcgcttt tttgttcgcc
    18421 gcccggtctt tgcctgggtt atcgccattt tgattatgtt ggcggggatt ctggctattc
    18481 gcacattgcc cgtcgcgcaa tatcctgacg ttgcaccgcc gaccattaaa atttcagcca
    18541 cttatactgg tgcttctgcc gaaacgctgg agaacagcgt gactcaggtt atcgagcaac
    18601 aactcactgg gcttgataat ttactctatt tcagctcaac cagtagctct gatggttcgg
    18661 tcagtattaa tgtgaccttt gaacaaggta ccgatccaga tactgcacag gttcaggtgc
    18721 agaataaaat tcagcaggcg gagtcgcgcc tacccagcga agtgcagcaa acgggtgtta
    18781 cggtggagaa atcacaaagc aactttttgc tgattgctgc cgtgtatgac accactgaca
    18841 aagcctccag ttcggatatc gccgactggc tggtcagtaa cgttcaggac cccctggcgc
    18901 gtgttgaagg tgtggggagt ctgcaagtct ttggcgcgga atacgccatg cgcatctggc
    18961 tcgacccggc caaactggcg tcttactcgc tgatgccttc agacgtgcaa agtgctattg
    19021 aagcgcaaaa cgtgcaggtt actgccggga aaataggggc attgccttca ccgaatactc
    19081 agcaactgac cgcaacggta cgtgcgcagt ctcgcttgca gacggtggat cagttcaaaa
    19141 atattatcgt gaaaagccag tcagacggcg cagttgttcg tataaaagat gtggctcgcg
    19201 ttgagatggg cagtgaagat tataccgcta tcggcaaact taacggtcac ccgtctgccg
    19261 gggttgctgt aatgctttcg cccggtgcga atgcgctgaa tacggcgacg ctggtcaagg
    19321 ataagattgc cgaattccag cggaacatgc cgcagggata cggcatcgca tacccgaaag
    19381 acagcactga atttattaaa atctccgtag aggacgtaat tcagacgctg tttgaagcca
    19441 tcgtattggt ggtttgcgtg atgtatttat tcctgcaaaa cctgcgtgcc acactgattc
    19501 cggcgttagc tgtccccgtc gttttgctgg gcacattcgg cgttcttgcg ttgtttggct
    19561 attcgattaa taccctgacg ttgtttgcaa tggtgctggc gatcggcttg ctggtggacg
    19621 atgccattgt ggtggtagag aacgtcgaac gcattatgcg tgacgaaggg ttgcccgcgc
    19681 gtgaagccac ggaaaaatca atgggcgaga tttctggcgc actggttgcc attgcgctgg
    19741 tgttgtcagc cgtattcctg ccgatggcct tctttggggg atctacgggg gtaatttatc
    19801 gtcagttctc catcaccatc atctccgcaa tgctgctttc cgtggtggtg gcattgacct
    19861 tgactcccgc cctgtgcggt tccgtcctcc agcatgttcc gccacataaa aaaggctttt
    19921 tcggcgcatt taaccgcttt tatcgccgta ctgaagataa atatcagcga ggcgtaattt
    19981 atgtcctgcg ccgtgcagcc cgaacgatgg ggctttatgt cgtgctgggt ggtggaatgg
    20041 cactgatgat gtggaagctg ccgggcagtt tcttacccac tgaagaccag ggcgaaatca
    20101 tggtgcagta cacgctgccg gccggggcga ccgctgcccg tacagcagaa gtgaatcgcc
    20161 agattgttga ctggttcctg attaacgaga aagcaaatac cgatgtcatc tttaccgttg
    20221 atggtttcag ttttagcggc agcggacaga acaccgggat ggcgtttgtt tcgttgaaaa
    20281 actggtctca acgtaaaggg gcagaaaaca ccgcccaggc tatcgcccta cgggctacca
    20341 aagagctggg cacaattcgt gatgccacgg tatttgcgat gacgccgcca gccgttgatg
    20401 ggctgggcca aagcaatggt tttacgtttg aattgttagc taacggtgga gccgatcgtg
    20461 aaacactgct gcaaatgcgt aatcagttga tagaaaaagc gaatcaaagt ccggagttac
    20521 attctgtacg cgccaatgat ttgccacaaa tgccgcaatt gcaggtagat attgatagta
    20581 ataaagcggt gtcattaggg ttgagtttga atgatgtcac cgacaccctg tccagtgcgt
    20641 ggggcggtac ttatgtgaat gactttattg accgtggtcg tgtgaaaaaa gtctacattc
    20701 agggcgacag cgaatttcgt tccgcgccgt cagacttagg taaatggttt gtgcgcggta
    20761 gcgataacgc catgacacca ttctctgctt ttgcgaccac ccgctggctg tatggaccgg
    20821 aaagactggt gcgctacaac ggctcggcag cctatgaaat tcagggcgaa aacgcgactg
    20881 gctttagttc cggcgatgca atgacaaaaa tggaggaact ggcaaacagt cttcctgcgg
    20941 gaacaacctg ggcctggagt ggtttgtcat tgcaggagaa actggccagc ggtcaggcat
    21001 taagtctgta tgcagtttct attctggtgg ttttcctctg ccttgcagca ctgtacgaaa
    21061 gctggtcagt cccgttctcg gtcattctgg tgatccctct ggggcttctt ggcgcggcgc
    21121 tggcggcctg gatgcgtgat ttaaacaacg acgtttactt ccaggtagcg ctattaacta
    21181 ctatcggctt gtcgtcgaaa aacgccattc tgattgtgga atttgctgaa gcggcggttg
    21241 cagagggcta ttccttgagt cgtgcggcat tacgcgcggc gcagactcgt ttacgcccaa
    21301 tcatcatgac ctcgctggcg tttattgcgg gggtaatgcc gctggcgata gcaaccggcg
    21361 caggggcgaa cagccgcatc gccattggta cgggcattat tggcggcacg ctgaccgcta
    21421 cattactggc cattttcttc gttcctctgt tttttgtact ggtgaaacgt ttgtttgccg
    21481 gtaaaccgcg ccgtcaggag taagttatgt tgcgtcgttc tcttattttt ctggtgttgt
    21541 tgagtgcggg atgtgtctcg ctcgatcctc actacagtac gcctgaatcc ccaatcccgg
    21601 cgaccttgcc gggagcacag ggccagggga aggcaatcag ccatgactgg cagcaggtga
    21661 ttcacgatcc ccgattgcag caggtagtaa ccattgcact gaacagtaac cgcgatgtgc
    21721 aaaaagcgat tgctgatatt gactcagcac gggcgctcta tgggcaaact aatgcgtcgt
    21781 tatttccgac ggtgaatgcg gccttaagtt cgactcgcag tcgttcgctg gcgaatggta
    21841 cgggaacaac tgccgaggct gacggcacgg tgtccagtta tacgttagat ctgtttggtc
    21901 gtaatcagag tttatctcgg gcggcgcggg aaacctggct tgccagtgaa tttacagccc
    21961 agaacactcg tttaacgctg attgctgaaa tcagcaccgc ctggctgaca ctggcggccg
    22021 ataacagtaa cctggccctg gcaaaagaaa cgatggccag tgcagaaaac tcattgaaaa
    22081 ttatccagcg ccagcaacag gttggcacgg cggcagcgac agatgtcagt gaggcgatga
    22141 gtgtttatca gcaagcgcgc gccagcgttg ccagctatca gacacaagtg atgcaggata
    22201 aaaatgcgct gaacttactg gcaggtacaa cgcttgcgga aaatctgcta ccgggaacac
    22261 tggaaagcct gccggaacaa atgataagtc tggttcctgc gggagtatca tctgatgttc
    22321 tgctgcgtcg cccggatatc caggaagcgg aacataattt gaagagcgcc aatgcagata
    22381 ttggcgcggc gcgggctaac tttttcccga cgatttcgct gactgccagt gccggtgtgg
    22441 gcagtgacgc attgtcgtcg ttgttcagcc acggaatgca gatctggtca tttgcgccat
    22501 cggtcacatt accgctgttc actggcggaa gtaatctggc gcagcttcgt tacgcagaag
    22561 cacaaaagcg tgggctcatt gccacctatg aaaaaaccgt tcagagcgca tttaaagatg
    22621 tcgctaacgc actggcgcgg cggaccacgc ttgaagagca actggatgca cagcgccagt
    22681 atgttaaagc tgagcaacaa acggtcgatg ttggcttgcg tcgctatcag gcgggtgttg
    22741 gtgattacct gaccgtgttg acggcgcagc gcagcttgtg gagcgcacag caggagctgc
    22801 tggcactgca actgactgat tttacaaacc gaatcacgct ctggcagtcg ctgggcggcg
    22861 gcatgtcatc actcaagtaa ggaataatat ggctagagtc tctctttcat gggcattgat
    22921 tcttggtctt ttagccggta tcggcccgat gtgtaccgat ctttatttgc cggctttgcc
    22981 ggagatgtct gagcaactgg cggcaaccac gaccataacg caattaaccc tgactgcatc
    23041 actgattggt cttggcgtcg gacaactgtt atttggccct ctgagcgaca aaatagggcg
    23101 taaacgtccg ttaatcttgt cgttgctatt gtttattgtt tcttccattt tgtgcgcgac
    23161 aacgaacaat atttactggc tggtggtctg gcgttttatt caagggatcg cgggggcggg
    23221 tggttcggtg ctctctcgtt ctattgctcg tgacaaatat cagggagtaa cgttgaccca
    23281 gttttttgcg ctgttaatga cggtgaatgg cctggcaccg gtgttgtcgc cagtgctggg
    23341 cgggtacatt gttagcactt ttgactggcg cactttattc tgggtaatgg ctgaaattag
    23401 caccgtactg ttgctgggct gcctgttatt tattaatgag accttgccag aaaataaaag
    23461 gggctcatca ttgctattaa ccggacgaag cgtggtgcag aaccgccgtt ttatgcgctt
    23521 ttgcctgatt caaagtttta tgctggctgg tttgtttgca tatatcggct cttcgtcgtt
    23581 cgtgttgcag aaggaatttg gctttagtcc aatgcaattt agcctggtgt ttggccttaa
    23641 cggcatcgga cttatcattg cttcatggat cttctcgcgc ctggcgcgac ggattaacgc
    23701 gatgatattg ttgcgaggtg gcctgatagc ggcaattttg tgtgcattgc tcacggtctt
    23761 atgcgcatgg gtacaattgc ccattccggc actggtggca ttatttttca ccatcgcatt
    23821 ttgtagcggc atcggcactg ttggcggggc agaggctatg agtgcagtag ggacgcagga
    23881 atctggaacg gcgtctgcgt tgatggggat gagcatgttt gtcttcggcg gtatagccgc
    23941 gccattgtcg ggaattggcg gagaaacact gttaaaaatg agtctggcaa ttacggtgtg
    24001 ttatacgctg gcattgctgg ttgctctcac cagaatcgac aatcaaaagt aatgaacacc
    24061 tcatcctgag gggggcctgt caggatgagg tgcaaaacac taacggtcct tacgccacgc
    24121 atccgccgtc aatgcttcgc caaaatgacc ggcgatcagc cgtttggtga gttcatgcag
    24181 cggcgatgcc agcacatctg cggtgctgcc tcgctcgaca acctcgccct gatgcatcac
    24241 cagcacctgg tcgctaatgt gcttcatcat tccgatatgc tgggtaacat aaatatacga
    24301 aatgccctgt ttttcctgta actccagcat cagattaatc aactgcgaac gcatcgacat
    24361 atccagtgag gcgagggctt catcggcaat aatgactttt gggcgcaata tcagcgcgcg
    24421 cgccagaccc agacgctgtt tttgcccggg tgctaacata tgcggatagt aactgacgtg
    24481 atccggtagc agcccaacca tacgcatcgt ttcaataatc tgtttgcgac gctgttccgg
    24541 ttccaggtcg gtgttcaggc gcagtggaaa atccagaatt tgcgagatac gttgacgggg
    24601 attcaacgag gtcgaaggat cctgaaaaat catgcgaata cgctgactgc ggaaggaata
    24661 atcgccaaaa tgcagcggat gatcgtcaat caataactcg ccgctggtcg gttctatcat
    24721 tcccgccagc atttttgcca gcgtggattt acccgaacca ttctcgccga taatcgccag
    24781 tgtctggcct tcacgtagcg taaagctcaa gggttttacc gcttctacgg tctgacgacg
    24841 aaaccagccg gtccggtagc ggaacgtttt acttaaattg cgcacttcaa gtagcgtttc
    24901 gatcatctca ctctttctcc atgttcagcg ggaaatgaca ggcatagaga tgatttttcg
    24961 cccccgtcaa acgtggcgtc acaatgcatt ctcgttgtgc atacgggcaa cgtggcccca
    25021 gacgacaacc aatcggtaac tgttccagca gcgggatagc gccgggcagc gtattgaggc
    25081 gacttttatg cggcatcgcg ctgccgaagt ctggtatcgc gcggatcagc gcctgggtat
    25141 aaggatggtg tggcatcgtc accaactcct tactcggcgc ggtttccacc gtttgaccgc
    25201 agtaaagcac gttaatttta tccgcccatt ggctaagcat ttgtaagtca tggctgataa
    25261 gcaaaatagt ggtattgctg ttttggttga gacgcgtcag caggcgaaag atttgcgcct
    25321 gggttgttgg ctccattgag ttggtcggtt cgtcagcaat tagcagacgt ggttgattcg
    25381 ccagtgcaat ggctatcatc actttctgac attcaccttc ggtcaactca tagggaaaac
    25441 tgcgcatcgc atctttgtga tctttaatcc ccacgcggtg cagcagttca atggcacggc
    25501 gtttgcgcca gccaaaacgc tgccaccaac ggcctttata ggtccaggca gggatgtttt
    25561 gcatcaactg gcgacccaca cgttctgaag ggtcaagaca cgactgtggt tcctggaaaa
    25621 tcatcgacac gttgtgacca accagcttgc gccgttcgcg tgcggagaga cgcagcaaat
    25681 cgatatcatc aaaacgcata cggtcagcag taacacgcca gttatcttta ttcaccccac
    25741 aaatcgcttt agcaatcaaa cttttgccgg aaccggattc gccaacaaga ccgcggattt
    25801 caccttcggt taaggtcata cttacgcggt cgacggcttt aacccactca tcaccggttt
    25861 taaattcaat ggtcaggtta cgaatatcga gtaatggcat tattccaccc ccgcaataat
    25921 tgcacgacgg acaccgtcgc caaggaggtt aaccaacagc acgctaatca taattgccgc
    25981 acctggcagc atgacagtcc acggggcgac ataaatcagt tccagcgcat caccgagcat
    26041 cgctccccat tcaggcgagg ggagttgtgc gccgagatcg agaaagccca gcgcggcgat
    26101 atcgagaatt gccatcgaca gtgcgcgggt gatctcggtg accagcccgg cggtgatgtt
    26161 gggcatcaca gcaaaccaga gaatattcag cgttgatgcg ccatccagac gggcggcgat
    26221 aacgtactct ttttccagtt cgtcatgcac catgctgtaa atcgaacgta ccatacgcgg
    26281 cagcagcgcc agccagacag caaacatggc gtgagacaaa ctcggtccgg caaacgctac
    26341 aacgataatt gccagcagca gcgaagggat cgccagtaag gtatccagaa tatggttaag
    26401 caccgctgag cgtaggccgt gggtcgcccc ggcgaaggtg cccagtacca ggccacagat
    26461 cgtcgcggca agcgtcacca caaatgcgcc acctacggtt ggcgcagctc cgctcagtaa
    26521 acggcttaaa acatcgcgtc cgaggtcgtc agtccccagg aagaaagaaa cttcgccata
    26581 gcgcgaccat gacggcggca ataattgata accgagaaat tgctggtcga tgccgtaagg
    26641 cgcaaaccag ccgccaaaaa tacacagtac agccagcccc gcgcagccgt acaggccgac
    26701 catcgcagag gcgtcactat aaaatttgcg ccaggcggta cgcagcgtgc ccggcgggcg
    26761 cttttcgctg tatacgctat cgtaaggcat accattcctt atgtttcaga gggttagcca
    26821 tggcacccaa aatatcagaa atcacgttaa caataatgac cagtgagcca cacaccatca
    26881 ctccggcgga aatggctgcg taatcctgct ggcggatggc gttaattaac cagcgcccta
    26941 aacccggcca gctaaaaacc atttcggtga tcatcgccag cgtcagcatg gtggaaaact
    27001 gtaagcccag gcgaggaata accggaggta acgcgttatg cagaacgtgg cgacgcaaaa
    27061 tagtaaagcg cgacaaaccg cgggttgccg ctgctttaac atagttctgg tcatacactt
    27121 cgatagtgct gatacgcatc agtcggatca cttcagttgt tggcgcaacc gacagggtga
    27181 tcacgggtaa tatcatatgg cggattgcgc tcatgaccat ttcatcccgc cacggtgagt
    27241 tcgaaagcca ggcatcaatc aacgcaaaac cggtaatcgg tttcacttcg tagagcaaat
    27301 cgaaacgccc agaaacgggc aaccaaccta gcgtgagtga acaaaacagc gtcagcagaa
    27361 gcgccagcca gaacaccggg attgaaaagc ccagtaaggc gatggcgttg atcaagttat
    27421 cctgccactt atggcgcgta atcccggcaa tcatccccac ggggatgcca acaatcagcg
    27481 caaaaccgaa ggcgaggatg cacagttcca tcgtcgccgg gaagacctct tttaactgtt
    27541 cggcaatcgg ttgaccgtta atgctggaaa caccaaaatc ccagtggatc aggccgttaa
    27601 accagaacac ccaggcattc cacagtgacg cgccttgtaa cggcgcatgt ggggtgaaat
    27661 agctcaagct gaagccaacg aaggtcagca ggaacaaggt cacaatcaat aacaaaatac
    27721 ggcgtaaggt gaagataatc atggcttttt cacctcatcc tgtttctcgc gatacacccc
    27781 agcaaaggag gcgttaccaa acgggctaag taccagacct ttgatatcgt accgataggc
    27841 ctgcaaacgc aatgacgacg ccagcggcag aataggtaat tcctgcgcca gaatactttg
    27901 cgcttcgtca taggcttcaa tacgcgccgc cagctgctgc gaagagagcg ccttacgcaa
    27961 cacgctgtcg aatttcggat cgcaccagtg ggcgaggttg gtctgcgaat gaattgctgc
    28021 gcagctcagt aacggacgga agaaactgtc cgggtcgtta ctgtccgtcg cccaaccgga
    28081 taacgtcaga tcatggctca tatccatcaa ccgcgcctcc tgaaaacgac cttctaccgg
    28141 cacaatcacc acttttacgc caacctgtgc catatccgcc tgaatcagtt cggcggtttt
    28201 cagtggactg gggttccacg cctgcgaacg tgtgggcacc cacagtttca gcgttaagtt
    28261 ttccagcccc aacgccttca actgttcgcg cgattttgcc ggattgtatt cagtaatttt
    28321 agcctcgttg tcataggccc acgaggctcg cggtaaaata gaggccgccg tttcagccgt
    28381 accataatag atggattgca tcaggcgctg gttattaatt gccagtgcca gcgcatggcg
    28441 gacagcggga ttatttagcg gcggtttagc ggtgttaaac gccagatagg cgacgttcat
    28501 ccccgggcgc agcgttaaac gcaagcgtgg gtcgtcacgc aaaatggata gctgactggc
    28561 agcaggccag gccagaacgt cgcactcccc ggtcaggagt ttcgacaatc gtcctgtacc
    28621 gccagagcct aaatccacca ccacttgtgg catcaacggc ttaccacgcc agaaatcatc
    28681 atgacgttgt aagcgaatat attgcccggc gcggtattcc gacaactgat acggaccggt
    28741 gccgaccggt tgacggtcga gttgctcctg gcgatcttct ttctctaact tccctgcata
    28801 ttctgccgac atgaccgaag cataatgggt cgccaggtgc cacaaaaaag aagcatccgg
    28861 ctgggcgaga cggaactcaa cggtatgatt atccagcttg cggacgcttt tcacgttatc
    28921 ggcaaattgc agactgtcga aataggggaa gttactgccg ttgacgttat gccacgggtt
    28981 gttgcggtca aaaatccgtt ggaaggtaaa caccacatca tcggcattca ttttacgagt
    29041 gggagtaaac cagtcggttt tttgaaacgg aacatcgcga cgcaggtgga agcgataggt
    29101 cgcgccgttg tcgagtactt cccagctttc ggcaagttcc ggcatcaggc gataggtata
    29161 gggatcgaca tccagcagtc gatcataaaa ctgggcggca agggtatcga caattaaccc
    29221 actgctcgct ttggatgggt taaaggtgtt gacttgcccg ctgacgcaat agacaaaacc
    29281 gctgtcgcgg atatcagcat gcggggggga ttcaggcgcg gcgattgcct gaccactcac
    29341 aagtccagca atcaccaaaa gagacgataa tacctggcgc ataatattaa gggattttat
    29401 gtaaagaggc tatcttacta atatttaatg acatttgcca ttaccgtttg tgttcagagg
    29461 tcgtaatgag ggccgtgtgg ttggtcattg aacaggtggc gtcgccatca gttcaacatt
    29521 cgtacccgtg atttctttat aactataact cacagaacaa cttagcgagg agcatagcgg
    29581 tacgatcgtg agaaacccat taaagcatgc tgcagtgcgt gtcattttta gccggatgcg
    29641 gcgcgaacgc tttatccggg caacgatact gaccgatagt ctgcaattaa ataattactc
    29701 attaccccat tgattcaaaa actctgcgat ctcatcaatg cgtacgggat taatccccgc
    29761 ttcagcagcc atttcatgtt gggcttcttc actgatctct tcattattca tcaaacgggt
    29821 gagcagtaac tggaagtagt gggccagcgg tgtgcgttca gcgtctgcga caggctctgc
    29881 gcattcggtc gcgtactcat cggcaatatc aaaatatttt agcgggatat cgtggttcat
    29941 tatttgcccc tggggttaat gcgctaagca taaaggcgga tgatagcact cgtgatccgg
    30001 gtcatacata tttttatagt taatggcgcg gtgcattacc ctcaggcagg ataatgcgcc
    30061 gcgcattcga ctattacgtt tcgctcaccg gcgttctgtc gccatcatac aacggcactt
    30121 tgcgatagcg gcggatcaaa taccacaaat atgcgccgcc taacgaagcc cataccatcc
    30181 cggttgcgca attcgatgcg gcaatgaaaa ttttgcagga tgcgctgaga gattaatacg
    30241 tagaagcagg gttaacgcaa taatgcctga tgcgcagcgc ttatcaggcc tgcaaacgac
    30301 gtattgattt tgtaggcctg ataaggcgtt cacggcacac ccggcaagtt gcattgtcga
    30361 aatgctaaat ctggtgcttt ttcaacaacg cgcggaactg atgataggtt aaccccagca
    30421 attcagcggc gcgcttctgg ttatatttcc cctgttgcaa actgagttgc agcaactctt
    30481 tttcctgctg catctgaaac tcgcgtaaat ccagtggcag cgtcggaagc gaggtgtttt
    30541 ctgaaacggc gatggcttct tcagatggac ggcgtttaaa gggatcaata atgatgtcat
    30601 caagcggata atcgctggtg ccgtgacgat acactgaacg ttccaccacg tttttcaatt
    30661 cacgtatatt tcccggccag cgataattca gcaatgtttc tctggcgtgc tcggtaaaac
    30721 ccgggaacag aggcagcttg atttcccgac acatctggat ggcaaagtgt tctgccatca
    30781 acataatgtc gctttcacgc tcgcgcagtg gtggcagttg cacaacatcg aacgctagtc
    30841 ggtcgagcag gtcagcgcga aaagtacctt cattgaccat cgccgggaga tcggcattcg
    30901 tcgcgcatac caaccgcaca ttcacctgca atggttggct gccgccgacg cgctccagtt
    30961 caccgtactc aatcacacgc aataattttt cctgcaccat catcggtgcc gtagcgagtt
    31021 catcaagaaa tagcgtaccg ccgtcggcac gttcaaatct ccctggatga cgtttttgcg
    31081 caccggtaaa cgcccccgct tcgtgaccaa acagttcgga atccagcaaa ttttcattta
    31141 acgccgcgca gttaagggaa ataaacggcc cttgccaacg ggaggagaga taatgcaggc
    31201 ggctggcgat cagctcttta ccggtgccgc gctcgccgat gatgagcacc ggtttgtcca
    31261 gcggtgcgag atgcgaaacc tgttccagca cttcgagaaa gctgttcgcc tcaccgagta
    31321 aattatcttt gtattctgcc atgatgaaat tcgccacttg ttagtgtaat tcgctaattc
    31381 atcctggcat gttgctgttg attcttcaat cagatcttta taaatcaaaa agataaaaaa
    31441 ttggcacgca aattgtatta acagttcagc aggacaatcc tgaacgcaga aatcaagagg
    31501 acaacattat gggtattttt tctcgctttg ccgacatcgt gaatgccaac atcaacgctc
    31561 tgttagagaa agcggaagat ccacagaaac tggtgcgtct gatgatccag gagatggaag
    31621 atacattggt tgaagtacgt tctacttcgg cgcgtgcgct ggcagaaaag aaacagctga
    31681 ctcgccgtat tgaacaagcg tcggcgcgtg aaattgaatg gcaggaaaaa gcggaactgg
    31741 cactgctgaa agatagagaa gatctggcgc gtgcggcgtt aattgaaaaa cagaaactga
    31801 ccgatctgat taagagcctg gaacatgaag tgacgctggt ggacgatacg ctagtgcgta
    31861 tgaagaaaga gatcggcgag ctggaaaaca aattgagcga aacacgcgct cgccagcagg
    31921 ccttgatgtt acgccatcag gcggcaaact cgtcgcgcga tgtgcgtcgt cagctggaca
    31981 gtggcaaact ggatgaagca atggctcgtt tcgaatcttt cgaacgccgt atcgaccaga
    32041 tggaagcgga agcagaaagc cacagcttcg gtaaacaaaa atcgctggac gatcagtttg
    32101 ccgaactgaa agccgatgat gcaatcagcg agcaactggc acaattaaaa gccaaaatga
    32161 agcaagacaa tcaataataa catccaggcg gcgtccgaac gcgccgccgc tcatcgtcta
    32221 aggagtcctt atgagcgcgc tatttctggc tattccgtta accatttttg tgctgtttgt
    32281 tttaccgatc tggttatggc tgcattacag caatcgttct ggtcgcagtg aattgtcgca
    32341 aagtgagcag cagcgattag cgcaactggc tgatgaagca aaacggatgc gcgaacgtat
    32401 tcaggcgctg gaatctattc ttgatgcaga acatccgaac tggagggatc gctaatggcg
    32461 ggcattaatc tcaataaaaa attatggcgt attccacagc agggcatggt ccgtggcgtc
    32521 tgcgccggga ttgccaacta ttttgatgta ccagtaaaac tggtgcgtat cctggtggtg
    32581 ctgtcgattt tcttcggtct ggcgctgttt accctggttg cttacatcat tttgtcattt
    32641 gcgcttgatc caatgccgga caacatggcc tttggtgagc agctaccttc cagcagtgaa
    32701 ttgctggatg aagtcgaccg tgaactggcg gcaagtgaaa cgcgtttacg cgagatggaa
    32761 cgttatgtca cttccgatac tttcacgtta cgtagccgtt tccgtcaact gtgaggaaag
    32821 ttatgaatac tcgctggcaa caggccgggc aaaaggtgaa gcctggtttc aaattagcag
    32881 gcaagctggt acttcttacc gcactgcgct atggcccggc gggtgtagct ggctgggcga
    32941 taaaatcagt tgctcgccga ccactgaaaa tgttgctggc tgtggcgctg gaaccgctgt
    33001 taagtcgggc tgccaataaa ctggcgcagc gttataaaag gtgaggggag tttcgcaaaa
    33061 attgttaaag ctaaggcgta ttatggatgg caattttcat ccatagaagg acgcttacat
    33121 gtttaaaaaa ggcttacttg ctctgacact ggtgttttca ctgcccgttt tcgccgctga
    33181 acactggatc gatgttcgtg ttccagagca gtatcagcaa gagcacgttc agggagccat
    33241 caatattccc ctgaaggaag tgaaagagcg cattgccacc gccgttccgg ataaaaacga
    33301 caccgtgaaa gtgtattgca atgccggacg ccagtcaggg caggcaaaag agatccttag
    33361 cgagatggga tatacccacg ttgagaacgc cggtggcctg aaagacatcg caatgccgaa
    33421 ggtcaaaggt taaaagaatc caccctaagc ggcgcttatg cgccgtttac tttttctgtc
    33481 acaccttatt tacatcccca tagattattt gcgtcagctc acaaatacgc tttttccctg
    33541 gtaaaaaatg atttcctgcg tgactaaaac ccttgtgctc aattgacagt ttattttctg
    33601 cggagtagtc tctcgtttca tgggaccgct accacggaaa ggcaacatgg aacagaaaat
    33661 tacggattac ctggacgaaa tctacggtgg aacatttacc gcaactcatt tacagaaact
    33721 tgtaacgcgt cttgagagtg cgaaacgatt aattacacag cgacgtaaaa aacactggga
    33781 tgaaagtgat gtcgtgttaa ttacctatgc tgatcaattt cacagtaatg atttaaaacc
    33841 actacccaca tttaatcagt tttaccctca atggctgcaa agaatttttt cacatgctca
    33901 tttattacct ttttatccat ggtcatctga tgatggattt tcggtaattg attatcatca
    33961 ggtcgccagt gaagcggggg agtggcagga tattcagcaa ctcggtgaat gcagccattt
    34021 aatgtttgat tttgtctgca accatatgtc ggcaaaaagt gaatggttta aaaactattt
    34081 acaacagcag ccaggttttg aagatttttt tattgccgtt gatccgaaaa ccgatctcag
    34141 cgccgtcact cgcccgcgtg cgttaccgtt attaacgcca ttccagatgc gcgatcattc
    34201 aatgcgccat ttatggacca cctttagcga cgatcaaatt gacctcaatt accgtagccc
    34261 tgaagtgttg ctggcgatgg tggatgtatt gctgtgctac ctggaaaagg gcgcagagta
    34321 tgtccgtctg gatgccgttg gctttatgtg gaaagagccg ggaacaaact gcatccatct
    34381 ggaaaaaaca catctgatta tcaaactgtt acggtcgatt attgataacg tcgcgccagg
    34441 cacagtgatc attaccgaga ccaatgttcc gcataaagac aacattgctt actttggcga
    34501 aggcgatgac gaagcgcata tggtgtacca gttctcgctg ccgccgctgg tgctgcatgc
    34561 ggtgcaaaaa cagaacgttg aggcactttg tgcgtgggcg caaaacctga cactaccttc
    34621 cagcaacacc acctggttta acttccttgc ctctcacgat ggcatcgggc ttaacccact
    34681 gcgggggttg ttgcccgaaa acgaaatatt agcgctggtc gaggtgttac agcaggaagg
    34741 agcattagta aactggaaaa ataatcccga cggtacgcgt agcccctatg aaatgaatgt
    34801 cacctatatg gatgcgttga gccgccgtga gtgtagcgac gaagaacggt gtgccaggtt
    34861 tattctcgcc catgcgattt tgttaagttt ccccggtgtg ccagcgatat atattcaaag
    34921 cattctgggg tcgcgtaatg attacgcagg tgtcgaaaaa cttggatata atcgtgcgat
    34981 taaccgtaaa aaatactcta gtaaagagat aacgacagaa ctgaacaata aagcgacatt
    35041 aagttattcg gtatattata agttgtcgca tttaattaca cttcgtcgca gtcataaaga
    35101 gtttcatccg gataataatt tcaccattga tgcgattaat tcatttgtaa tgcgaattca
    35161 aagaagcaat gcggatggcg attgcctgac gggattgttt aatgtcagtg aaaatattca
    35221 gcatataaat attactgacc tgcacggtcg ggatctgatt agtgaagttg atatattggg
    35281 taatgaaata acactgcatc cctggcaggt tatgtggatt aaataaaaag gaacatttca
    35341 tgattaaatc aaaaatcgtg ctgttatcag cactggtttc ctgcaccctg atttcaggct
    35401 gtaaagaaga aaataaaaat aatgtatcca tcgaatttat gcattcttcg gtggagcagg
    35461 agcgccaggc cgtcatcagc aaattgattg cccgttttga aaaagaaaac ccaggtatca
    35521 ccgtaaaaca agtgcccgtg gaagaagacg cctataacac caaagtcatt accctttcac
    35581 gtagcggttc gctgccggaa gtgatcgaaa ccagccatga ctacgccaaa gtgatggaca
    35641 aagagcagct tatcgatcgc caggcggttg ccacggtcat cagcaacgtc ggtgaaggcg
    35701 cgttctacga tggcgtactg cggattgtgc gcaccgaaga tggcagcgcc tggaccggtg
    35761 ttcctgtcag cgcatggatt ggcgggatct ggtatcgcaa agatgtgctg gcaaaagctg
    35821 ggctggagga gccaaaaaac tggcagcaat tgctggacgt tgcacagaaa ctgaatgacc
    35881 cggcgaataa aaaatacggt attgcgctgc ctacagcaga aagcgtgttg acggaacagt
    35941 ctttctccca gtttgcgtta tccaatcagg ctaacgtctt taacgccgaa ggcaaaatca
    36001 cccttgatac accagagatg atgcaggcgc tgacctatta ccgcaacctt gctgccaaca
    36061 ccatgccagg ttctaacgac atcatggaag tgaaagacgc ttttatgaac ggcaccgcgc
    36121 cgatggcgat ttactccacc tatatccttc cggctgtgat taaagaaggc gatccgaaaa
    36181 acgttggttt cgtggtgcca accgagaaaa attctgcggt ctacggcatg ttgacctcgc
    36241 tgaccattac cgccgggcaa aagactgaag agaccgaagc ggctgaaaaa tttgtcacct
    36301 ttatggagca ggcggacaac attgccgact gggtgatgat gtcaccaggc gcggcgctgc
    36361 cggtgaacaa agcggtggtg actaccgcca cctggaaaga caacgacgtt attaaggcgc
    36421 tgggagaatt gccgaatcag ctaatcagcg aactgccaaa tattcaggtc tttggcgcag
    36481 taggggataa aaactttacc cgcatgggcg atgtgacggg gtctggcgtg gtgagttcaa
    36541 tggtgcataa cgtcaccgtg ggtaaagccg atctccctgg cacgctgcaa gcgagccaga
    36601 aaaagctgga tgaactgatc gaacagcact aaacccagga caggaatccg caatgaacag
    36661 gcttttttca ggtcgttccg atatgccttt tgcactgctg cttctcgcgc ccagcttatt
    36721 actgctgggc ggtctggtgg cgtggccgat ggtgtcgaat atcgaaatca gttttttacg
    36781 tctgccgctt aatcccaata tcgaggcaac atttgttggg gtgagcaact atgtgcgtat
    36841 tctctccgat cctggtttct ggcactcgct gtggatgacg gtctggtata ccgcgcttgt
    36901 ggtggcgggc agcaccgtgc tcgggctggc cgtggcgatg tttttcaacc gcgagttccg
    36961 cctgcgcaaa accgcgcgtt cgctggtgat cctttcctac gtaacgccgt ctatttcgct
    37021 ggtgttcgcc tggaaataca tgttcaacaa cggctacggc attgtgaact acctcggcgt
    37081 tgatcttctg catctctatg agcaggcacc gctgtggttc gataatccgg gcagcagctt
    37141 tgtgctggtg gtgctgttcg ccatctggcg ctacttcccc tatgccttta tttcgtttct
    37201 ggcgattttg cagaccatcg acaaatcgct gtacgaagcg gcagagatgg atggcgcgaa
    37261 tgcctggcag cggtttcgca tcgtcacgct gcccgcgatt atgccggtcc tggcgacggt
    37321 ggtgaccctg cgcactatct ggatgttcta catgttcgcg gatgtttatc tgctgacgac
    37381 caaggtcgat attctcggtg tctatctcta caaaaccgcc tttgccttta atgacttagg
    37441 gaaagcggcg gcgatctcgg tggtgctttt catcattatt ttcgctgtca ttctgctgac
    37501 caggaaacgg gtgaacctca atggcaacaa ataaacgcac actcagtcga atcggttttt
    37561 actgcgggct ggcgctgttt ctcatcatca cgctgtttcc gttttttgtg atgctgatga
    37621 cctcgttcaa gagcgcaaaa gaggcgatct cgctgcatcc tacgctgctg ccacagcagt
    37681 ggacgctgga gcattacgtc gacattttta atccggtgat tttcccgttt gtcgactact
    37741 tccgtaacag catggtggtg tcggtggttt catccgtggt ggcggtattt ctcggcattc
    37801 ttggggctta cgcgctttcc cgcctgcgct ttaaagggcg gatgaccatc aacgccagct
    37861 tttacacggt gtacatgttc tccggcattt tgctggtggt gccgctgttc aaaatcatca
    37921 ccgcacttgg catttacgac accgagatgg cgctgattat caccatggtg acgcaaaccc
    37981 tgcccaccgc tgtgtttatg ctgaaaagct acttcgacac cattcctgat gagatcgaag
    38041 aagcggcgat gatggatggc ctcaatcgtc tgcaaattat cttccgtatc accgtgcccc
    38101 tggcaatgtc cgggctgata tccgtattcg tctactgctt tatggtggcg tggaacgact
    38161 atctgtttgc gtcgattttc ctttccagcg ccagcaattt caccttaccg gtgggcctga
    38221 acgcgctgtt cagtacgcca gattacatct ggggacggat gatggcggcc tcgctggtga
    38281 ccgcattgcc ggtggtgatt atgtacgcgc tttctgaacg ttttattaag agtggtttga
    38341 ccgccggtgg cgtgaagggc taaagcggcc agtttcttta caaggagttt tacatgaaaa
    38401 agttagtagc cacagcaccg cgtgttgctg cgctggttga gtatgaagag cgggcgattt
    38461 cagccaatga agtgaagatc cgcgtgcgtt tcggcgcgcc aaaacacggt actgaagtgg
    38521 tcgatttccg cgccgccagc ccgtttatcg atgaagaatt taatggcgaa tggcagatgt
    38581 tcaccccgcg tctggcagat gcgccgcgcg gcattgagtt tggcaagttc cagcttggca
    38641 acatggtggt tggcgacatt atcgagtgcg gcagcgacgt taccaactac gcggtgggcg
    38701 acagcgtatg cggctacggc ccgctctccg agacggtcat cattaacgca gtgaataact
    38761 acaagctgcg caaaatgccg caaggcagct cctggaaaaa cgccgtctgc tacgacccgg
    38821 cgcagtttgc catgagcggc gtgcgcgatg ccaatgtacg cgtaggggat tttgtagtgg
    38881 tggtcgggct tggcgcgatc ggtcaaattg ccatccaact ggcaaaacgc gctggagcgt
    38941 cggtggtaat tggcgtcgat cctattaccc gtcgttgtga tattgcccgt cgccacggtg
    39001 cggatttctg tcttaacccc attggcgctg acgtaggtaa agagatcaaa acgctgaccg
    39061 gcaagcaggg tgccgatgtg attatcgaaa ccagtggcta tgccgacgcg ctgcaatcgg
    39121 cactccgcgg tctggcctat ggcggcacca tctcctatgt cgcgtttgcc aaaccgtttg
    39181 ccgaaggttt taacctcgga cgcgaagccc atttcaataa cgccaaaatt gttttctccc
    39241 gtgcgtgcag cgagccgaac ccggattatc cgcgctggag ccgtaagcgt attgaagaaa
    39301 cctgctggga actgctgatg aacggttatc tcaattgcga agatttaatc gatccggtgg
    39361 tgacctttgc caacagcccg gaaagctaca tgcagtatgt cgaccagcat ccggaacaga
    39421 gcatcaaaat gggcgtcacg ttttaattca aaggaatgaa aagaatgaaa atcggtacac
    39481 agaatcaggc gttctttccg gaaaacattc tggagaaatt tcgttatatc aaagagatgg
    39541 gtttcgatgg ttttgagatt gacggcaaac tgctggtcaa caatctcgaa gaagtcaaag
    39601 cagcaatcaa agaaaccggt ttaccggtga ccaccgcctg cggtggctat gacggttgga
    39661 ttggcgactt tatcgaagag cgtcgactta atggcttaaa gcagatcgaa cgcattcttg
    39721 aagcgctggc agaagtgggc ggtaaaggta tcgtcgttcc ggctgcatgg ggcatgttta
    39781 ccttccgctt accgccgatg acctcgccgc gtagcctgga cggcgaccgc aaaatggtga
    39841 gtgattccct gcgcgtactg gaacaggtcg ccgcgcggac tggaactgtg gtgtatctcg
    39901 aaccgttaaa ccgctatcag gatcatatga tcaacaccct cgccgatgct cgtcgttaca
    39961 tcgtcgaaaa caatcttaaa catgtgcaga ttatcggcga tttctatcac atgaatatcg
    40021 aagaagataa cctggcgcag gcgttgcatg acaaccgcga cttgctcggt catgtgcata
    40081 ttgccgataa ccatcgctac cagccgggca gcggcaccct cgatttccac gcgctgtttg
    40141 aacagctgcg cgccgataac tatcagggct atgtggtgta tgaagggcgt atccgggcag
    40201 aaaatcctgc ccaggcgtac cgtgattcgt tggcctggtt gcgcacctgc taagaggtct
    40261 ttgtgaaaag tgcaatgaca agctctccgc tgcgagtcgc gatcataggc gcgggccagg
    40321 tggcggataa agttcatgct tcgtactact gcacccgcaa cgatctggaa ctggtggctg
    40381 tctgtgacag ccgcctttcc caggcgcagg cgctggcaga aaaatacggg aatgcatccg
    40441 tgtgggacga tccgcaggcc atgctgctgg cggtgaaacc tgatgtggtt agcgtctgct
    40501 cacctaaccg ttttcattac gaacataccc tgatggcgct ggaagcgggc tgccatgtga
    40561 tgtgcgaaaa accgcccgcc atgacggcag aacaggcgcg ggaaatgtgc gataccgtgc
    40621 gcaaacaggg caaggtgctg gcctacgact ttcaccatcg ttttgcactt gatacgcaac
    40681 agctgcgtga acaggtgacc aacggcgttt tgggagagat ttacgttacc accgcccgcg
    40741 ccctgcgtcg ttgcggtgtt cccggctggg gtgtctttac taataaagaa ttgcagggcg
    40801 gaggcccgct gatcgacatc ggcattcata tgctggatgc ggcgatgtat gtgctgggtt
    40861 ttccagcggt gaaaagcgtg actgcgcata gctttcaaaa gatcggcacg caaaagagct
    40921 gcggtcaatt tggcgagtgg gacccggcaa cctacagcgt cgaagattcg ctgtttggca
    40981 ccattgaatt tcataacggc ggcattctgt ggcttgagac ctcatttgca ctcaacatcc
    41041 gcgaacagtc gattatgaac gtcagctttt gtggtgataa agccggggcg acgctgtttc
    41101 cagcacatat ctacaccgat aacaacggtg agttaatgac gcttatgcaa cgggaaatgg
    41161 cagacgacaa ccgtcatctg cgcagtatgg aagcctttat caaccacgta caaggtaagc
    41221 ccgtgatgat agccgacgcc gagcagggat acatcatcca gcaactggtg gcggcgttgt
    41281 atcaatccgc agaaacaggg acgcgtgtgg aattatgatc aggccagtaa cgttaacgga
    41341 accccatttc agccagcata ccctgaacaa gtatgcttcg ctgatggcgc aggggaacgg
    41401 ctatcttggg cttcgcgcca gccatgaaga agattacacg cgccagacgc gcggcatgta
    41461 tctggcaggg ctttatcatc gggcgggtaa aggtgaaatc aacgaactgg tgaacctgcc
    41521 tgatgtcgtg gggatggaga ttgccataaa tggtgagatt atctcgttat cccgcgaagc
    41581 atggcagagt gagcttaact ttgccagtgg cgaattacgt cgcaacgttg tctggctacc
    41641 agcaacggca ctggttacac catcgccagc cgtcgctttg tttcggcaga ccaactgccg
    41701 ctgatcgcgc tggaaatcag tattaccccg ctggacgcca acgcgtcagt gctgatttca
    41761 acaggcattg acgccacgca aaccaatcac ggtcgccagc atctcgacga aacccaggtg
    41821 cgggtgtttg gtcagcatct gatgcagggg atctacacca cccaggatgg gcgcagtgat
    41881 gtggccatca gctgttgctg taaggtgagc ggtgatgtgc agcaatgcta taccgccaaa
    41941 gagcgccgtt tactgcaaca tacctgcgcg cagcttcacg caggtgagac gttgacgctg
    42001 caaaaactgg tgtggatcga ctggcgggac gacagacaag ccgctttaga cgaatggggc
    42061 agtgcgtcgc ttcgtcagct tgaaatgtgc gcgcagcaga gttacgacca acttcttgca
    42121 gcatcaacag aaaactggcg tcaatggtgg cagaaacgtc gtatcacggt aaacggcggc
    42181 gatgcgcacg atcagcaagc gttagattat gcgctttatc acctgcgcat catgacgcct
    42241 gcccacgatg agcgcagcag cattgcggcg aaaggtttaa ccggcgaagg ctacaaaggc
    42301 cacgttttct gggatacaga agtatttttg ctaccgttct atctgtttag cgatccgatg
    42361 gttgcccgaa gtttactgcg ttatcgctgg cacaacttgc ctggcgcgca ggagaaagcg
    42421 cgacgcaacg gctggcaggg cgcgctattt ccgtgggaaa gcgcgcgtag cggcgaagaa
    42481 gagacgccgg aatttgctgc cattaacatt cgtaccgggt tgcggcaaaa agtggcctct
    42541 gcgcaggcgg aacatcatct ggtggccgat atcgcctggg cggttattca atactggcgg
    42601 accacggggg atgaaagttt cattgctcat gaaggcatgg cgctacttct ggagacggca
    42661 aagttctgga ttagccgcgc ggtgagggtt aacgaccgtc tggaaattca tgatgttatt
    42721 gggccagacg aatataccga acatgtcaat aataacgcct tcaccagcta tatggcgtat
    42781 tacaacgtcc agcaggcgct gaatatcgct cgtcagtttg gctgtagcga cgacgcgttt
    42841 attcatcgtg cagaaatgta cctcaaagaa ctgcggctgc cagaaattca gcccgacggc
    42901 gttttgccgc aggatgattc gtttatggct aagccggtga ttaatctggc gaaatacaaa
    42961 gcggcggcgg ggaagcaaac cattctgctg gattattcac gcgcagaagt gaacgagatg
    43021 cagatcctca aacaagctga tgtggtgatg ctcaattaca tgctgccgga gcagttctca
    43081 gcggcatcgt gtcttgccaa tctgcaattt tatgaaccgc gcactattca cgactcgtca
    43141 ttaagtaaag caatccacgg cattgttgcc gcacgctgtg gcctgctgac ccaaagttat
    43201 cagttctggc gcgaggggac tgaaatcgat cttggtgctg atccacatag ctgtgatgac
    43261 ggtatccacg ctgccgcaac tggcgctatc tggctggggg cgattcaggg ttttgccggg
    43321 gtgagcgtgc gtgacggtga attacatctc aatccggcgt tacctgagca gtggcaacag
    43381 ttgtctttcc ctctgttctg gcagggctgc gaattacagg tcactctcga cgcgcagcgt
    43441 attgcgattc gaacttctgc gcccgtttca ctgcgcttga acggtcagct tatttacgtc
    43501 gctgaagaat ctgttttctg tttgggggat tttattttgc ccttcaatgg gaccgctacc
    43561 acgcatcagg agggtgaatg aaactgcaag gggtaatttt cgatctggat ggagtaatca
    43621 cagatagcgc acatctgcat ttccaggcgt ggcagcagat tgccgctgaa attggcatca
    43681 gcattgatgc gcagtttaac gaattcctaa aagggatcag ccgcgatgag tccctgcggc
    43741 gaattctgca gcacgggggt aaagagggcg attttaaccc acaggaacgg gcgcaactgg
    43801 cgtatcgcaa aaatctgctt tacgtccact cactgcgcga actgacggtt aatgctgtgc
    43861 tgcctggcat tcacaatttg ctggtagatc tccgagcaca gcagattccg gttgggctgg
    43921 cttctgtctc cctgaatgcg ccgacgattt tagcagcgct ggagctgcgc gagtttttca
    43981 ccttctgcgc ggacgcttcc caactcaaaa attcgaaacc ggacccggaa atctttctcg
    44041 ccgcctgtgc agggctgggc gtgccgccac aggcatgtat cggcattgaa gatgcgcagg
    44101 cgggcattga agccattaac gccagcggta tgcgctcggt ggggatcggc gcgggcttaa
    44161 ccggggcgca attattgttg ccctcaacgg actccctgac ctggccgcgg ttatcggcct
    44221 tctggcaaaa cgtatagcaa aggaatcaac atggcacagc tttcgttaca acatattcaa
    44281 aagatctacg ataaccaggt gcatgtggtg aaggacttca acctggagat tgccgataaa
    44341 gagttcatcg tgtttgtcgg tccgtcgggc tgcggtaaat cgaccaccct gcgtatgatt
    44401 gccgggcttg aggagatcag cggcggcgat ctgttgatcg acggcaaacg aatgaatgac
    44461 gttccagcca aagcacgcaa tatcgcgatg gtgttccaga actacgcgct gtatccgcat
    44521 atgacggtct acgacaacat ggcgtttggc ctgaagatgc aaaaaatcgc cagagaggtg
    44581 attgatgagc gggtgaactg ggcggcgcaa attctcggcc tgcgtgagta cctgaaacgc
    44641 aagccgggcg cgctttccgg cgggcaacgt cagcgagtgg cgcttgggcg ggcgattgtg
    44701 cgcgaagcgg gcgtgttttt aatggatgag ccgctctcta acctggatgc caagctgcgc
    44761 gtgcaaatgc gcgcagagat cagcaagctg catcagaaac tgaacaccac catgatctac
    44821 gtgacccacg atcagaccga agcgatgacc atggcgacgc ggattgtcat tatgaaggat
    44881 gggattgttc agcaggtcgg tgcgccgaaa acggtttata accaacctgc gaatatgttt
    44941 gttgccggat ttattggatc accagcgatg aattttattc gcggcacgat cgatggcgat
    45001 aaattcgtta cggaaacgct taaattaacc atacccgaag agaaattagc ggttctgaaa
    45061 acacaggaaa gtttgcataa gcccatcgta atgggaatac gcccggaaga tattcatccg
    45121 gacgcgcaag aggaaaataa catttccgcc aaaattagcg tggcggaatt aaccggtgcg
    45181 gaatttatgc tctacaccac ggttgggggg cacgagttag tggtccgtgc tggtgcgtta
    45241 aatgattatc atgcaggaga aaatatcact attcattttg atatgactaa atgtcatttc
    45301 tttgatgcag aaacggaaat agcaatttgc taaatacagg gggaaggcat tctcccagga
    45361 taatacaagg aacaataatg aaaaagttat tactctgtac cgcactggtg atgtgtgcgg
    45421 gaatggcctg cgcacaggcc gaggaaaaga acgactggca ctttaatatc ggcgcgatgt
    45481 acgaaataga aaacgtcgaa ggttatggcg aagatatgga tgggctggcg gagccttctg
    45541 tctattttaa tgccgccaac gggccgtgga gaatttctct ggcctattat caggaagggc
    45601 cggtagatta tagcgcgggt aaacgtggaa cgtggtttga tcgcccggag ctggaggtac
    45661 attatcagtt cctcgaaagc gatgatttca gtttcggtct gactggtggt ttccgtaatt
    45721 atggttatca ctacgttgat gaaccgggta aagacacggc gaatatgcag cgctggaaaa
    45781 ttgcgccaga ctgggatgtg aaactgactg acgatttacg tttcaacggt tggctgtcga
    45841 tgtataaatt tgccaacgat ctgaatacga ctggttatgc tgatacccgt gtcgaaacgg
    45901 aaacgagtct gcaatatacc ttcaacgaaa cggttgcctt gcgagtgaac tattatctcg
    45961 agcgcggttt caatatggat gatagccgca ataacggtga gttttccacg caagaaattc
    46021 gcgcctattt gccgctgacg ctcggcaacc actcggtgac gccgtatacg cgcattgggc
    46081 tggatcgctg gagtaactgg gactggcagg atgatattga acgtgaaggc catgatttta
    46141 accgtgtggg tttattttac ggttatgatt tccagaatgg tctttccgtt tcgctggaat
    46201 acgcgtttga gtggcaggat cacgacgaag gcgacagcga taaattccat tatgcgggtg
    46261 ttggggtgaa ttactcgttc tgataatgtg ctaaattgcc ggatgcgggg cgagcgccaa
    46321 atccggcata tatttatttc aacgacatat gcgtagcaac ggtaatattt tgtggtgacg
    46381 gttttccggc aattagctgg aataataact cgcagctttg ttgacctaac tcctgcgtcg
    46441 gaacatcgat gccgcctggt gcaggcgtta atataaacga cagcgtttca ttactataac
    46501 ccaccaccgc taactgctgc ggaatagcaa tgtttttctc tgccgccgca cgataaatac
    46561 tcattaattt caggctgtca gtagcaaaca ctgcctcagg caacggtgac tggcttaata
    46621 attcccgtgc tgcttttaat gctgtctcat gggtataacc gccgtcaaca atccattcat
    46681 cacgcactgc aatattatgc gtagccaggc tttgcttata accatttgcc cgatccactg
    46741 aaacatgaac atcaagcggc gcatgcaggc atgcaatatt cttatgcccg ctttcaatta
    46801 acgcatcggt tagcgcaata ctgtcgccat aattatcggt atcgacggaa taaacatggc
    46861 tgtattgacc ttcaacttta ccgatcacca ccaccgggat ctcgtatttg tcgagttgtg
    46921 caaagaagga ttcatcggca ggcgaactta gcataataat gcctttaatc attttctgct
    46981 taattttgct ttcgcatttt tgcagatctt ctgctggatt gtgcgacgtt tgtaatatca
    47041 cgtcgaaacc ttcttcttcc gctttggcgg tgatggcatg caaaacttca gagaaaaacg
    47101 gattacccgc cgtagttttg gtcgaacggg tagaaatcac cataatggca tcaaaacccg
    47161 aagaggtcag cgcgcgggcc agcttgtttg gttgatactg taattcttca atggcccgta
    47221 acactttttc gcgcgcttcc ggggagatat tggtttgctt attcagcacg cgtgatacgg
    47281 tggattttga tacgcctgca accctggcaa tatcataaat agtaggggac ataggtcagg
    47341 aactccgtcc gaacgtgaat gctgcacatc ttatggagtt cgcccgtcga tgacaacacg
    47401 ggtagttgat aatcaatggc ctggccccca cattcatatc cttacgaacg attttttttc
    47461 tggtcttcag agcagcacag gacagcaatg aagcgactta aaaatgaact caatgcgctg
    47521 gtgaatcggg gtgtcgacag acatctgcgc ctcgctgtaa ccggacttag ccgcagcggc
    47581 aaaacagcgt ttatcaccgc gatggttaat cagttgctca atattcatgc cggagcacgt
    47641 ttgccgcttt taagtgcggt gcgtgaagag cgcctgctgg gcgtaaaacg cattccccag
    47701 cgtgactttg gcattccgcg cttcacctat gatgaagggt tggcgcagtt atatggcgat
    47761 cctcccgcct ggccgacgcc tacgcgcggc gtcagtgaaa ttcgcctggc gttacgtttt
    47821 aaatcgaacg attcgctgct gcgccacttt aaggacacct ccacgctgta tcttgagatt
    47881 gtggattatc ccggcgaatg gctgctcgac ctgccgatgc tggcacagga ctattttagc
    47941 tggtcgcgcc agatgacggg cttactcaat ggtcagcgcg gcgaatggtc ggcgaaatgg
    48001 cgaatgatgt gcgaagggct ggacccgcta gcgcctgccg acgaaaaccg gctggcagac
    48061 attgccgccg cgtggaccga ttatctccat cactgcaaac agcaggggct gcactttatt
    48121 cagccaggac gctttgtttt gcctggcgat atggcaggag cgcccgcgct gcaattcttc
    48181 ccatggccag atgtcgatgc ctggggcgag tctaaactgg cgcaggccga taaacacacc
    48241 aacgccggaa tgttgcgcga gcgttttaat tattactgcg agaaggtggt gaaaggattc
    48301 tataaaaatc acttcctgcg ttttgaccgc cagattgtgc tggtggactg cctgcaaccg
    48361 ctcaacagtg gaccacaggc atttaatgat atgcgtctgg cgctgacgca gctgatgcaa
    48421 agtttccact acgggcagcg taccctgttc cggcgtttgt tttcgccggt tatcgataag
    48481 ctattgtttg ctgccactaa agcggaccat gtgaccatcg atcagcacgc caatatggtt
    48541 tcattgctgc aacaactgat tcaggatgcc tggcaaaatg cggcgttcga agggatcagt
    48601 atggactgtc tggggctggc gtctgttcag gcgaccacca gcggcattat tgatgttaac
    48661 ggtgagaaaa tcccggcgct gcgcggtaat cgactcagcg atggcgcacc gctcactgtt
    48721 tatcctggcg aagttcccgc acgtttgcca ggtcagacat tctgggataa gcaaggcttc
    48781 cagtttgagg cgtttcgtcc gcaggtgatg gatgtcgaca aaccgctacc gcatattcgt
    48841 ctagatgctg cgctggaatt tttaatagga gataaattgc gatgaccgaa ccgttaaaac
    48901 cacgtattga tttcgacggt ccactggagg tcgatcagaa tccaaaattc agggcgcagc
    48961 agacctttga cgaaaatcag gcgcaaaatt ttgccccagc cacgctcgat gaagcgcagg
    49021 aagaagaggg gcaagttgaa gcggtaatgg atgcagcgtt acgtccgaaa cgcagcctgt
    49081 ggcgcaaaat ggtgatgggc gggctggctc tgtttggcgc aagcgttgtc gggcagggcg
    49141 tgcagtggac aatgaacgcc tggcaaaccc aggactgggt ggcgctgggt ggatgtgccg
    49201 ctggggcatt gattatcggc gctggcgtag gttctgtggt aacagagtgg cggcgcttat
    49261 ggcgcttgcg acagcgcgcc catgaacgcg acgaagcgcg cgatttgttg cacagtcacg
    49321 gcacgggtaa aggccgtgca ttctgcgaaa aactggcgca gcaggcgggt attgatcagt
    49381 cacatccggc gctgcagcgc tggtatgcct caatccatga aacgcagaac gatcgtgaag
    49441 tggtcagttt gtatgctcac ctggtccagc cagttttaga tgcccaggcg cggcgcgaaa
    49501 tcagccgttc ggcagcggaa tcaacgttaa tgattgccgt tagcccactg gcgctggtgg
    49561 atatggcgtt tatcgcctgg cgcaatctgc gtttgattaa tcgcatcgcc acgctgtatg
    49621 gcattgaact gggatattac agccgtttgc gcctgtttaa gctggtattg ctgaatattg
    49681 cttttgccgg agccagcgaa ctggtgcgcg aagtggggat ggactggatg tcgcaagatc
    49741 tcgctgcgcg tttgtctacc cgggcagctc aggggattgg tgctggactt ctgacggcac
    49801 gactggggat taaagctatg gagctttgcc gcccgctgcc gtggattgac gatgacaaac
    49861 ctcgcctcgg ggatttccgt cgtcagctta tcggtcaggt gaaagagacg ctgcaaaaag
    49921 gcaaaacgcc cagcgaaaaa taatgcaata tcgggtgctg accggatatc tttacgccga
    49981 agtgcccgtt tttccgtctt tgtgtcaatg attgttgaca gaaaccttcc tgctatccaa
    50041 atagtgtcat atcatcatat taattgttct tttttcaggt gaaggttccc atgcgtctgg
    50101 aagtcttttg tgaagaccga ctcggtctga cccgcgaatt actcgatcta ctcgtgctaa
    50161 gaggcattga tttacgcggt attgagattg accccattgg gcgaatctac ctcaattttg
    50221 ctgaactgga gtttgagagt ttcagcagtc tgatggccga aatacgccgt attgcaggtg
    50281 ttaccgatgt gcgtactgtc ccgtggatgc cttccgaacg tgagcatctg gcgttgagcg
    50341 cgttactgga ggcgttgcct gaacctgtgc tctctgtcga tatgaaaagc aaagtggata
    50401 tggcgaaccc ggcgagctgt cagctttttg ggcaaaaatt ggatcgcctg cgcaaccata
    50461 ccgccgcgca attgattaac ggctttaatt ttttacgctg gctggaaagc gaaccgcaag
    50521 attcgcataa cgagcatgtc gttattaatg ggcagaattt cctgatggag attacgcctg
    50581 tttatcttca ggatgaaaat gatcaacacg tcctgaccgg tgcggtggtg atgttgcgat
    50641 caacgattcg tatgggccgt cagttgcaaa atgtcgccgc ccaggatgtc agcgccttta
    50701 gtcaaattgt cgccgtcagc ccgaaaatga agcacgttgt cgaacaggcg cagaaactgg
    50761 cgatgctaag cgcgccgctg ctgattacgg gtgacacagg tacaggtaaa gatctctttg
    50821 cctacgcctg ccatcaggca agccccagag cgggcaaacc ttacctggcg ctgaactgtg
    50881 cgtctatacc ggaagatgcg gtcgagagtg aactgtttgg tcatgctccg gaagggaaga
    50941 aagggttctt tgagcaggcg aacggtggtt cggtgctgtt ggatgaaata ggtgagatgt
    51001 caccacggat gcaggcgaaa ttactgcgtt tccttaatga tggcaccttc cgtcgggttg
    51061 gcgaagacca tgaggtgcat gtcgatgtgc gggtgatttg tgctacgcag aagaatctgg
    51121 tcgaactggt gcaaaaaggc gtgttccgtg aagatctcta ttatcgtctg aacgtgttga
    51181 cgctcaatct gccgccgcta cgtgactgtc cgcaggacat catgccgtta accgagctgt
    51241 tcgtcgctcg ctttgccgac gagcagggcg tgccgcgtcc gaaactggcc gctgacctga
    51301 atactgtact tacgcgttat gcgtggccgg gaaatgtgcg gcagttaaag aacgctatct
    51361 atcgcgcact gacacaactg gacggttatg agctgcgtcc acaggatatt ttgttgccgg
    51421 attatgacgc cgcaacggta gccgtgggcg aagatgcgat ggaaggttcg ctggacgaaa
    51481 tcaccagccg ttttgaacgc tcggtattaa cccagcttta tcgcaattat cccagcacgc
    51541 gcaaactggc aaaacgtctc ggcgtttcgc ataccgcgat tgccaataag ttgcgggaat
    51601 atggtctgag tcagaagaag aacgaagagt aagcgcgaat atgcctgatg gtgcaacacc
    51661 atcaggcata ttaacttatg ctttcagtac agccagagct gcttcgtaat ccggctcggt
    51721 ggtgatttca tccaccagct ggctgaaaat cacattgtca ttttcatcaa taaccacaac
    51781 ggcacgcgct gccagacctt tcagtgggcc atcagcaatt gccacaccgt aggcttgcag
    51841 gaattcagcg ttacggaaag tggagagggt gataacgttg ttcagacctt ctgcgccgca
    51901 gaaacgagac tgggcgaacg gcagatcggc agagatacac agcacaacgg tgttgtcgat
    51961 ctcggttgcc agttggttaa acttacgtac tgatgcggcg caaacaccgg tatcaatact
    52021 cgggaaaatg ttcagcactt tgcgtttacc cgcaaactga ccgagggtga cgtcagacag
    52081 atcttttgcc acgagagtaa aagtctgcgc tttgctaccc gcctgcggga tggaattggc
    52141 gactgtaacc gggttgccct ggaaatgaac ggtttgtgac atgattatct ttcctgttta
    52201 catatagtta acgtcacacc tagtttatgc taactgtcaa taacacagca aacgctattt
    52261 gcgcttaatc cgcagaccac cgcgacaaca aggagtaaag atgagaaccg ttaaggtatt
    52321 tgaggaagcc tggcccttac ataccccgtt tgtgattgct cggggaagtc gcagtgaagc
    52381 gcgcgtggtg gtggttgaac tggaagaaga gggtattaaa ggcaccggcg aatgcacgcc
    52441 gtatccgcgt tatggggaaa gtgatgcctc agtaatggcg caaattatga gcgtcgtgcc
    52501 gcaattagag aaagggctga cacgggagga gttgcaaaaa atcctccctg ccggcgcagc
    52561 acgtaatgcg ctggattgtg cattgtggga tctggcggcg cgaaaacagc agcaatcgct
    52621 ggctgctttg atcggcataa cgcttcccga gacagtcacc acggcacaga cgatagtcat
    52681 cggtacgcct gatcagatgg ccaatagtgc atcaacactc tggcaggcag gcgcgaaatt
    52741 actgaaagtg aagctggata accatcttat cagtgagcgg atggtggcaa ttcgcacagc
    52801 tgtgcccgat gcgacgctga tcgttgatgc aaatgaatct tggcgtgcag aagggttggc
    52861 ggcgcgttgc cagctattgg cggatttagg cgttgcgatg cttgaacaac cgcttcctgc
    52921 gcaggacgat gcggcgctgg agaattttat tcatccgtta ccgatttgtg ctgatgaaag
    52981 ttgtcatact cgtagcaatc tgaaggcgct gaaagggcgc tatgagattg ttaacattaa
    53041 gcttgataaa accggtggtc tgacggaagc gctggcgctg gcgactgaag cgcgtgcaca
    53101 aggttttcgt ctgatgctgg gctgcatgtt gtgtacctct cgtgccatta gcgccgcttt
    53161 accgctggtg ccgcaggtca gtttcgccga tcttgacgga ccgacctggc tggcggtaga
    53221 tgtggaaccg gcgcttcagt tcacgacggg cgaattgcat ctttaggatg ccagcgcagc
    53281 aagtttgcca tcgcaaagag atatttttcg ctggcttcgt cggaggagat gggtggaaac
    53341 tctgcggtga tgcaatgcaa gttgagatct gcacaccaac tgccgaaaga gccaggcgtt
    53401 tcataaccga cgctggttac cagaggcaat tcaaacgcct gggccagcca ttcacctaat
    53461 tcgctgtgtc tgggatcttc aatacaggcc agcggatcgt ggaatgagac cacccaggca
    53521 ggttgaatgc ggtgtataag ctggcacagc gcctgggttt caggttcgga gccaggtttg
    53581 tcacctgtca gtaaaacgac atcacgttct tcagcggcgc tgttccagcg ataaactgtt
    53641 tcaccttcct tccagttcgc tgccggaaag tttcggttta aatccacacc attagcattg
    53701 gcccgtaacc ctaactggca gccgtcagga ttcacacaca gcaccacatg atggcggcgc
    53761 aaagaaggtg tcaaagtccg cagagcacag gagagcgtga cgatcgaaga gttttcatca
    53821 ccgtgagtgc cagccagaat caaaccactt tcgtgactgg ctgcaggggc cggaaaccag
    53881 attaacggcg cgcccaataa tgaacgtccg taatgttctg ttccggtcgg aaatgcgccg
    53941 cgttcggcgc gtgggcgagt tacggtcatg atcgtctctg gttcaggaag tattacaggc
    54001 agtgttgtgc aaattttgcg gattatcaaa tccttttctt caacgctatg tcatcgatct
    54061 tgtatcactt ttttgccgga agttgtttgt attttcattg atgtgcagat attagaattg
    54121 tcgtaatgtt atatacatgg cgtattttat tcttactggt tctacacatg ctaatcgcat
    54181 ctgattgatc agttcacctt aataagttta ataaatcgta ctgcacatag tacagtacga
    54241 tggtagctat ataattgatt tacatgaaga agggcttatc accatcagtc ccgggttgat
    54301 tgatgcctat attctcacca atgtgtttct caggtttatc gatgatatct gtgattaatg
    54361 ctgcgacact cttacgtgat acgatcgtac ctttgaatgg ttcattgcgt gaggtaagct
    54421 cataatcaat aatatcttca tcagtcagcc aggcagggcg aagaattgta tattccagtc
    54481 cagaagcctc aattgcatcc gcagccctgc ggaatggttt taggggttca ccaataactg
    54541 cattattcca ttcgccaaat tttcctggaa cttcatcata aataccgagg gaaagaacaa
    54601 agatcagacg tttaacatca caggctttca tcgcagcgat cactgaattt gcctgaatat
    54661 ctaaatcttc ccccgtaagg ttggcataca caacatcctg tccctgcatg gcctgcttca
    54721 gtgctgcatg attcagtaca tcccccataa taatcttgct attagttgga tagggtttgt
    54781 ggatttttgc tggctgacgg gcaaataaag tttgtttaat ggtttgttta tctgccagtt
    54841 gattaatcac atggcgggct atctgaccgc cagcaccaag aatcagtaca tttttcatga
    54901 aacttttccc agattattgg cagcaatatg ccaggccata tcactattac ggatttgaat
    54961 atgtttcatt acagagtttc ctcaaagaac ggagctaata ctgaaattgc ttcagccaca
    55021 tagactttgc catcataaag atccatatgg tttgcgcctt caacaatgtg atagcgttta
    55081 tcctggcttg atgctcgatc gtacaggtcg tcactcatcc atttgctccc agcctggctg
    55141 cccgccacaa tctgtatcgg ctgagtcagg tacacttccg ccatatggta agcatcatag
    55201 gtaataatct ggttaaggct gcgcaaagta gcgtaacccg gtgctgttgg atactgcgcg
    55261 cgaggggtgt ggtaatattc ccaggcctga cgcagttctt cgttcggcgc atcggactcc
    55321 ttcattggtg ccagtggcat agtggcgtat tctccgctgc taatatcgct ggttcgggcg
    55381 tttgaacccg cgtcaacgta tggtagggca tcaatagatt tcacattgtt ttcccaacca
    55441 ttacggaaca tcgaaccaat attgaccgca ctaacagtac cgatggcctt gatgcggcga
    55501 tcctgaattg cagcattggc tgtatatcct gcaccggcac aaattcccat cgcaccaatt
    55561 cgggtattgt cgacataaga aagcgttgtc aggtaatcaa tcacggcact gacgtcttca
    55621 gtacgaatgt atgggttttc taactgacgc ggctcgccgc cgctttcacc ttgataagat
    55681 gcgtcataag caatagtgac aaatcccttt tccgccagtt tttcggcata ggttccggcc
    55741 gtttgctctt taacgccccc acctgggtga gataccacaa ttgccggata ctgacgggtt
    55801 tcatcaaatt ttggagggaa atagatcact gcagacaaag agatggttgg attattgctg
    55861 ttagtgaagc tgactttatt attcatcatt ccgttcctca tggagttgtc ggttcgtttt
    55921 aacggttggt gatatcacta tagatattga tcattaagtt gattagaccc taaatcatga
    55981 ttagacctat catttaaatg attaatagat tgtatggaga tactgaatga agcgtgaaga
    56041 aatcgctgat ctgatggcgt ttgtcgttgt tgcagaggag cgtagcttca ctcgcgcagc
    56101 agcccgcctg agcatggcgc agtcagcttt aagccagata gtgcgccgta tagaagaacg
    56161 attgggattg cggcttctga cgcgaaccac gcgcagcgtt gtacccactg aagcgggcga
    56221 gcatcttttg tctgttcttg gccctatgtt gcatgacata gattctgccc tgacatccct
    56281 gagcgatctg cagaaccgcc catccgggac aatacgtatt actactgtag aacatgcagc
    56341 aaaaacgata ttgttaccag ctatgcgcac attcctgaaa tcgcatcctg aaattgatat
    56401 tcagcttacc attgattatg gtttgaccga tgtcgtttct gaacgttttg atgcaggcgt
    56461 ccgtctgggt ggggagatgg ataaagatat gatcgccatt cgaatcgggc cagatatacc
    56521 aatggctatt gttggctcac cggattattt ttctcgccga agtgctccaa cgtcagtgtc
    56581 acaattaata gatcatcagg caattaattt gtatcttccc acatcgggta cagcaaatcg
    56641 ctggagatta atacgcggtg gacgtgaagt tcgtgttcgc atggaaggtc agcttttact
    56701 gaatacgata gacctgatca ttgatgctgc aattgatggg catggattgg cgtatctacc
    56761 ttatgatcag gttgagcggg ctattaaaga aaaaaaactg atacgtgttt tggataaatt
    56821 cacaccagat ttacccggtt atcacctgta ctatccacac cgtcgacatg ctggctcggc
    56881 attctcatta tttatagata ggctgaagta taaaggtgct gtttagcact acttgctgat
    56941 acattaattt aattcttctc ttaacgtatt ctcagttcct ttcaacgttt tggttatttt
    57001 ttattcttcg cacaatggcg acagatgctg attatgatac cgaaaacggg tttgaacgtg
    57061 cgaagcccga acgtagtgtt cggagttcta tgtgctttac cgcattttgg agactattat
    57121 ttacactaaa tctgatttga tatattgata cttaaaacat ttgatgcttc ctttgtcact
    57181 tttttgatgg aagttgtttg catttcttta aggcgaaaca aataattacg catcaatttt
    57241 aatgtcggtt agagggaaac ttatgaagca ctctgtttca gtcacgtgtt gtgcgctgtt
    57301 ggtcagcagc atttctcttt cgtatgctgc tgaagttccg agcggcacag tactggcaga
    57361 gaagcaggag ctggtgcgcc acattaaaga tgagccagcg tcgctggatc ccgctaaagc
    57421 cgtgggcctg ccggagattc aggtcattcg cgatctgttt gaaggactgg tgaatcagaa
    57481 cgagaaaggg gagattgtcc ccggcgttgc gactcagtgg aaaagtaatg acaaccgtat
    57541 ctggactttt accctgcgcg ataacgcaaa atgggcggat ggcacaccgg taacggcgca
    57601 agattttgtc tacagctggc aacgtctggt ggacccgaaa acgctatcgc catttgcatg
    57661 gtttgccgcg ctggcgggaa tcaacaacgc acaggcgatt attgatggta aagccgcgcc
    57721 tgaccagctt ggcgtcaccg cagttgatgc ccatactttg aaaatccagc ttgataaacc
    57781 gttgccgtgg tttgtgaact taaccgctaa ctttgccttc ttcccggtgc aaaaagccaa
    57841 cgtagaaagc agtaaagagt ggacgaaacc cggaaaactg atcggtaatg gcgcttatgt
    57901 tcttaaagag cgcgtagtca atgaaaaact ggtcgtggta ccgaataccc attattggga
    57961 taacgccaaa acggtgctgc aaaaagtgac cttcctgcca attaatcagg aatccgcagc
    58021 cactaagcgt tatctcgcgg gggatattga tatcaccgaa tccttcccga aaaatatgta
    58081 tcagaagctg ttgaaggata ttccggggca ggtttatacg ccgccgcagc tcgggaccta
    58141 ttattatgcg tttaacacgc aaaaagggcc gacggccgat cagcgtgttc gcctggcatt
    58201 aagtatgacg atagatcgcc gcctgatgac cgaaaaagtg ttgggaacgg gcgaaaagcc
    58261 agcgtggcat tttacaccag atgttaccgc gggatttacg ccggaacctt cgccgtttga
    58321 acaaatgagt caggaagaac tgaatgcgca ggcaaaaact ttgttgagcg cagctggtta
    58381 tggtccgcaa aaaccgctga agctgacgct tttgtataac acttcagaaa accatcaaaa
    58441 aattgcgatt gctgtagcat caatgtggaa aaagaacctt ggcgtagatg ttaaattgca
    58501 aaatcaggaa tggaaaacct atatcgatag ccgtaacacc ggcaattttg atgttatccg
    58561 agcctcgtgg gtaggggatt ataatgaacc ctccactttc ctgacattat taacgtcaac
    58621 gcattcagga aatatttcac gctttaacaa tccggcatac gacaaagtac tggcccaggc
    58681 atcaacggaa aataccgtta aagcgcgtaa tgccgattac aatacggcag aaaaaatcct
    58741 catggagcaa gcaccgattg caccaattta tcaatatacc aatggacgat taatcaagcc
    58801 gtggctgaaa ggttatccca ttaataaccc ggaagatgtg gcgtacagtc ggactatgta
    58861 tattgtgaag cattgatgtg atgggaactg gcgttaccct tgtggataac gccagtgatg
    58921 tctgatttag cgaccctggt ccggcggtgt aatgttatcc atatacagcg tctggctggg
    58981 gaaggcaaag tccgcgccgt gtgactgtac aatatcgata atcttcaaat aaacgtcttg
    59041 ctgtgcagca agccattcag cccataccgt ggttttggta aagcaataaa ccataatatt
    59101 caatgaagag tcagcaaact ggttgaaata aaccagtaag gtttgtcgct ggtcgatggc
    59161 cgggtgattt ttcagcatct cacgtacggc ttcgacaata acgcccactt ttgccgcatc
    59221 ctcataacgt aaaccaatgg tcgtggtaat gcgccggttg gtcattcgtc cggggttttc
    59281 tacgctgatt gacgaaaaca acgagttcgg tacgtacaac ggacgattat caaaagtcgt
    59341 aattttggta attcgccagc caatttccgc tactgtacct tcgatatttc tgtccggtga
    59401 acggatccag tcgccaatac taaaaggacg gtcgaaatag agcataatcc cggaaaagaa
    59461 gttactcaaa atatctttac cggccatacc gacagccaga ccaccaatac caccaaaggt
    59521 caacaagcca gaaaggctca tgccgaaatg ttcaccataa agcagaacaa gcaccacaat
    59581 aatggtgatt ttgatgatac gcgacataat ccgcgcactg gtgatatcgc gacctttttt
    59641 aatctgctgt ttttcaaact gattaatcag caaaaatagc ttaatcgtca gaataaccgc
    59701 aatcagggac gtacagataa aatcgataac gcctggggtg ataaatttga gtttatagtt
    59761 ttcaataaca taattaatga tgctaccaac agcactgata attatggtgt agattaaaaa
    59821 ttgcaccgca tggaataaaa aaccttttct tttacgattt ccgcggcgaa accaaaagct
    59881 catcagaatc aatgctgcgc agctaccgaa aataatgacc agattaagcg cattatttgt
    59941 aaacagttca gcgatcattg ttttatcagg ctcctccaga taattgtcgt catgccggaa
    60001 acccctggcg gggctatttt accgcgacaa ttcattcaga tcatcaatag tcagggaagg
    60061 aagtagcaac attagctaac taatcaatgc gttgattgta aatccagcta agaggtgagg
    60121 ttttcagagc agacaacggt gaaatgtcat ggtattgtta cgtttaggta acaagaaatt
    60181 tgtctgcaca aggattacat catgattatg gcgaaactga agtcagcgaa agggaagaaa
    60241 tttctctttg gtttgttggc ggttttcatt attgcggcgt cggttgtgac tcgcgcgacc
    60301 atcggcggcg ttatagaaca gtacaatatt ccgctgtctg agtggacgac atcaatgtat
    60361 gtgattcagt catcgatgat ttttgtttat agcctggtct ttactgtgtt gctggcaatc
    60421 ccgttgggaa tttatttcct tggcggcgaa gagcagtaag taaaaaatag gcccgataac
    60481 tcgggccttg tcagttattg aagagtcgtt aatcgtcttc ttcgtcatcc agttcaacgg
    60541 gtgtctgata ctggtcaggt ttaatgacca gcaggtcgca gcgaagatga tcaatcacct
    60601 gttccgccgt gttgccaagg aatgctgctg aaataccggt gcgtccgacc gagcccagaa
    60661 ccacaatccc cgcctgtaag tgctccgcca aatcaggaat cacctcttcg ggcagacctt
    60721 tttctacgtg cgtcatgttt tcattaatgc cgaatttctg ccgcagagct ttcattgcca
    60781 gcaaatgttg cccacgaatg gcatcgttat aaacgctcgg gtcaaattcc ggcagttcaa
    60841 tcgcgatatt aattggcgtt accggataag cgccaaccag atgaacttcg gtatggttga
    60901 cttgttctgc cagttcgatc gtctctttga ccagtttttc attgagcgca ttatggtacg
    60961 gctcttcact ggcgagattc accgccacca gcgccttacc cccttccggc cacggctggt
    61021 ctttcaccat ccacaccggg cttgggcatt tgcgtaacag atgccagtcc gttggcgtaa
    61081 aaatcaccgc ttccagacgg tcatgttggt gcgccatttt tagcaccaaa tcgtgcccgc
    61141 cgctgatcac ttcctgaatg atggcttcga aaggacggtt atgccagacc actttaattt
    61201 caatgggaac gccagcattg agataatatt ttgcctgctc gtggatccag gctgtacgct
    61261 ggctgatgac gccctgacgc atagcggtac gttcgtccgg agagagcagg gtggtcattt
    61321 cgtatgagaa gtcatagatc ggcaaaaaga ctttaatttt gccaccaatc cgttgatgta
    61381 aataaacagc tcgccgcaat gctggttggt cgtcctggtt aggatcgata acaacgagca
    61441 tgttctgata catagccata cagggtctcc ttacaacaac tgtcaacgca gtttgtaatt
    61501 aaaagattaa cccatatctg gtgaatgaaa cagtgatgaa ccttctgtca gatcaataaa
    61561 tcagaaaaat ttaatgatat gacagaagga tagtgagtta tgcggaagaa tcaggcaacg
    61621 ttacgcgtat gaccagcaag ctgggccagc gcatcgttat tttcgatagt gatgtattta
    61681 cctttgactg ccagcatacc gcttttctgg aagcgaccca gcagacggct gatggtttca
    61741 accgtcaggc ccagatagtt accgatatca ccacgagtca tcgtcaggcg gaattcacga
    61801 ggggagaagc cgcgttgggc aaaacgacgg gacaggttgt agatgaatgc agccagacgt
    61861 tcctcggcat ttttcttcga caacagcagg atcatgtcct gatcgccttt gatttcaccg
    61921 ctcatcagac gcatcatctg ctgacgcaga ttcggcattt taccagacaa atcgtccagc
    61981 gtttcgaacg ggatttcaca taccatcgag gtttccagcg cctgcgcgaa actcgggtga
    62041 tgaccgctgc cgatggcatc aaatcccacc agatcgcctg ctaaatggaa accagtgatt
    62101 tgctcgtcgc cttgctcagt gatggtataa cttttaatcg taccggagcg gatggcataa
    62161 agcgatttta gttcatctcc agccttaaac agcgtctggc ctttctgaat aggcttcttc
    62221 cgctcaatga tattatcaag ctgatcaagc tcatgttcgt tgagtgtgaa cgggatgcaa
    62281 agctggctga tgctgcaatc ctggcaatgg atagcacaac cgccagactg aatgcgccgt
    62341 ataattcgct tttccgggat cataggtctg ctcaagccgt aattgatatt tgtcaatttt
    62401 aacatctttt tagggagcaa gtaagtctaa gcaaacctta acagcagaga attccgatat
//
LOCUS       DADSYM010000004        62394 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2415
            SAMN08039579-rid11723173.denovo.004, whole genome shotgun sequence.
ACCESSION   DADSYM010000004 DADSYM010000000
VERSION     DADSYM010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039579
            Sequence Read Archive: SRR6362533
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 62394)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 62394)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980162.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 03:39:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,484
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 4,347
            CDSs (with protein)               :: 4,347
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 43 of 85
            Pseudo Genes (incomplete)         :: 37 of 85
            Pseudo Genes (internal stop)      :: 19 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..62394
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039579-rid11723173.denovo.004"
                     /strain="LGE2415"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8302 N 95.9628 W"
                     /collection_date="2015-08-24"
     gene            <1..144
                     /locus_tag="J1Y83_000191"
     CDS             <1..144
                     /locus_tag="J1Y83_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfL family protein"
                     /protein_id="HBA7628381.1"
                     /translation="LVGAVLATLTACTGHIENRDKNCSYDYLLHPAISISKIIGGCGP
                     TAQ"
     gene            complement(252..1337)
                     /locus_tag="J1Y83_000192"
     CDS             complement(252..1337)
                     /locus_tag="J1Y83_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HBA7628382.1"
                     /translation="MKTFPLQSLTLIEAQQKQFALVDTICRHFPGSEFLTCGDLGLTP
                     GLNQPRITQRVEQVLADAFHAPAAALVQGAGTGAIRAALAALLKPGQRLLVHDAPVYP
                     TTRVIIEQMGLTLITADFNDLSALKQVVDEQQPDAALVQHTRQQPQDRYVLADVLATL
                     RAAGVPALTDDNYAVMKVSRIGCECGANVSTFSCFKLFGPEGVGAVVGDADVISRIRA
                     TLYSGGSQIQGAQALEVLRGLVFAPVMHAVQAGVSERLLALLNGGAVAEVKSAVIANA
                     QSKVLIVEFHQPIAARVLEEAQKLGALPYPVGAESKYEIPPLFYRLSGTFRQANPQLE
                     HCAIRINPNRSGEETILRILRESIASI"
     gene            complement(1349..2653)
                     /locus_tag="J1Y83_000193"
     CDS             complement(1349..2653)
                     /locus_tag="J1Y83_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA7628383.1"
                     /translation="MDLYIQIIVVACLTGMTSLLAHRSAAVFHDGIRPILPQLIEGYM
                     NRREAGSIAFGLSIGFVASVGISFTLKTGLLNAWLLFLPTDILGVLAINSLMAFGLGA
                     IWGILILTCLLPVNQLLTALPVDVLGSLGELSSPVVSAFALFPLVAIFYQFGWKQSLI
                     AAVVVLMTRVVVVRYFPHLNPESIEIFIGMVMLLGIAITHDLRHRDENDIDASGMSVF
                     EERTSRIIKNLPYIAIVGALIAAVASMKIFAGSEVSIFTLEKAYSAGVTPEQSQTLIN
                     QAALAEFMRGLGFVPLIATTALATGVYAVAGFTFVYAVGYLSPNPMVAAVLGAVVISA
                     EVLLLRSIGKWLGRYPSVRNASDNIRNAMNMLMEVALLVGSIFAAIKMAGYTGFSIAV
                     AIYFLNESLGRPVQKMAAPVVAVMITGILLNVLYWLGLFVPA"
     gene            complement(2665..3018)
                     /locus_tag="J1Y83_000194"
     CDS             complement(2665..3018)
                     /locus_tag="J1Y83_000194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417837.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2620 domain-containing protein"
                     /protein_id="HBA7628384.1"
                     /translation="MKKIGVAGLQREQIKKTIEATAPGCFEVFIHNDMEAAMKVKSGQ
                     LDYYIGACNTGAGAALSIAIAVIGYNKSCTIAKPGIKAKDEHIAKMIAEGKVAFGLSV
                     EHVEHAIPMLINHLK"
     gene            complement(3029..3907)
                     /locus_tag="J1Y83_000195"
     CDS             complement(3029..3907)
                     /locus_tag="J1Y83_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotriesterase-related protein"
                     /protein_id="HBA7628385.1"
                     /translation="MSFDPTGYTLAHEHLHIDLSGFKNNVDCRLDQYAFICQEMNDLM
                     ARGVRNVIEMTNRYMGRNAQFMLDVMRETGINVVACTGYYQDAFFPEHVATRSVQELA
                     QEMVDEIEQGIDGTDLKAGIIAEIGSSEGKITPLEEKVFIAAALAHNQTRRPISTHTS
                     FSTMGLEQLALLQAHGVDLSRVTVGHCDLKDNLDNILKMIDLGAYVQFDTIGKNSYYP
                     DEKRIAMLHALRDRGLLNRVMLSMDITRRSHLKANGGYGYDFLLTTFIPQLRQSGFSQ
                     ADVDVMLRENPSQFFQ"
     gene            complement(3904..5130)
                     /locus_tag="J1Y83_000196"
     CDS             complement(3904..5130)
                     /locus_tag="J1Y83_000196"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HBA7628386.1"
                     /translation="MARFVVLVIDSFGVGAMKDVTLVRPQDAGANTCGHILSQLPHLQ
                     LPTLETLGLINALGYAPGDMQPSDSATWGVAELQHEGGDTFMGHQEILGTRPLPPLRM
                     PFHDVIDRVEQALVSAGWQVERRGDDLQFLWVNQAVAIGDNLEADLGQVYNITANLSV
                     ISFDDAIKMGRIVREQVQVGRVITFGGLLTDSQCILDAAESKEGRFIGINAPRSGAYD
                     NGFQVVHMGYGVDEKVQVPQKLYEAGVPTVLVGKVADIVSNPYGMSWQNLVDSQRIMD
                     ITLNEFNTYPTAFICTNIQETDLAGHAEDVARYAERLQVVDRNLARLVEAMQPDDCLV
                     VMADHGNDPTIGHSHHTREVVPVLVYQQGLVATQLGVRTTLSDVGATVCEFFRAPPPQ
                     NGRSFLSSFRFAGDTL"
     gene            complement(5131..6294)
                     /locus_tag="J1Y83_000197"
     CDS             complement(5131..6294)
                     /locus_tag="J1Y83_000197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfX family PLP-dependent enzyme"
                     /protein_id="HBA7628387.1"
                     /translation="MFVEALKRQNPALISAALSLWQKGKIAPDSWVIDVDQILENGKR
                     LIETARLYGIELYLMTKQFGRNPWLAEKLLALGYSGIVAVDYKEARVMRRAGLPVAHQ
                     GHLVQIPCHQVSDAVEQGTDVITVFTLDKAREISAAAVKTGRLQSVLLKVYSDDDFLY
                     PGQESGFVQHSLHEVVAEIQNLPGLHLAGLTHFPCLLWDEAAGKVLPTPNLHTLVQAR
                     DQLAKSGIAIEQLNAPSATSCTSLPLLAEYGVTHTEPGHALTGTIPANQQGDQPERIA
                     MLWLSEISHHFRGDSYCYGGGYYRRGHAQHALVFTPENQRITETYLNAVDDSSIDYTL
                     PLAGEHPVSSAVVLCFRTQIFITRSDVVLVSGIHRGEPEIVGRYDSLGNPLEA"
     gene            complement(6378..6740)
                     /locus_tag="J1Y83_000198"
     CDS             complement(6378..6740)
                     /locus_tag="J1Y83_000198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417841.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HBA7628388.1"
                     /translation="METRLNLLCDAGVIDKDICKGMMQVVNVLETECHLPVRSEQGTM
                     AMTHMASALMRSRRGEEIEPLDNELLAELAQSSHWQAVVQLHQVLLKEFALEVNPCEE
                     GYLLANLYGLWMAANEEV"
     gene            complement(6757..7662)
                     /locus_tag="J1Y83_000199"
     CDS             complement(6757..7662)
                     /locus_tag="J1Y83_000199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA7628389.1"
                     /translation="MRRTFIKKEGVVITTLARYLLGEKCGNRLKTIDELATECRSSVG
                     LTQAALKTLESSGAIRIERRGRNGSYLVEMDNKALLTHVDINNVVCAMPLPYTRLYEG
                     LASGLKAQFDGIPFYYAHMRGADIRVECLLNGVYDMAVVSRLAAESYLTQKGLCLALE
                     LGPHTYVGEHQLICRKGESANVKRVGLDNRSADQKIMTDVFFGDSDVERVDLSYHESL
                     QRIVKGDVDAVIWNVVAENELTMLGLEATPLTDDPRFLQATEAVVLTRVDDYPMQQLL
                     RAVVDKHALLAHQQRVVSGEQEPSY"
     gene            complement(7822..8826)
                     /gene="trpS"
                     /locus_tag="J1Y83_000200"
     CDS             complement(7822..8826)
                     /gene="trpS"
                     /locus_tag="J1Y83_000200"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HBA7628390.1"
                     /translation="MTKPIVFSGAQPSGELTIGNYMGALRQWVNMQDDYHCIYCIVDQ
                     HAITVRQDAQKLRKATLDTLALYLACGIDPEKSTIFVQSHVPEHAQLGWALNCYTYFG
                     ELSRMTQFKDKSARYAENINAGLFDYPVLMAADILLYQTNLVPVGEDQKQHLELSRDI
                     AQRFNALYGDIFKVPEPFIPKSGARVMSLLEPTKKMSKSDDNRNNVIGLLEDPKSVVK
                     KIKRAVTDSDEPPVVRYDVQNKAGVSNLLDILSAVTGQSIPELEKQFEGKMYGHLKGE
                     VADAVSGMLTELQERYHRFRNDEAFLQQVMKDGAEKASAHASRTLKAVYEAIGFVAKP
                     "
     gene            complement(8819..9577)
                     /gene="gph"
                     /locus_tag="J1Y83_000201"
     CDS             complement(8819..9577)
                     /gene="gph"
                     /locus_tag="J1Y83_000201"
                     /EC_number="3.1.3.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycolate phosphatase"
                     /protein_id="HBA7628391.1"
                     /translation="MNKFEDIRGVAFDLDGTLVDSAPGLAAAVDMALYALELPVAGEE
                     RVITWIGNGADILMERALTWARQERATLRKTMGKPPVDDDIPAEEQVRILRKLFDRYY
                     GEVAEEGTFLFPHVADTLGALQAKGLPLGLVTNKPTPFVAPLLEALDIAKYFSVVIGG
                     DDVQNKKPHPDPLLLVAERMGIAPQQMLFVGDSRNDIQAAKAAGCPSVGLTYGYNYGE
                     AIDLSQPDVIYQSINDLLPALGLPHSENQESKND"
     gene            complement(9570..10247)
                     /gene="rpe"
                     /locus_tag="J1Y83_000202"
     CDS             complement(9570..10247)
                     /gene="rpe"
                     /locus_tag="J1Y83_000202"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HBA7628392.1"
                     /translation="MKQYLIAPSILSADFARLGEDTAKALAAGADVVHFDVMDNHYVP
                     NLTIGPMVLKSLRNYGITAPIDVHLMVKPVDRIVPDFAAAGASIITFHPEASEHVDRT
                     LQLIKENGCKAGLVFNPATPLSYLDYVMDKLDVILLMSVNPGFGGQSFIPQTLDKLRE
                     VRRRIDESGFDIRLEVDGGVKVNNIGEIAAAGADMFVAGSAIFDQPDYKKVIDEMRSE
                     LAKVSHE"
     gene            complement(10265..11101)
                     /gene="dam"
                     /locus_tag="J1Y83_000203"
     CDS             complement(10265..11101)
                     /gene="dam"
                     /locus_tag="J1Y83_000203"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine-specific DNA-methyltransferase"
                     /protein_id="HBA7628393.1"
                     /translation="MKKNRAFLKWAGGKYPLLDDIKRHLPKGECLVEPFVGAGSVFLN
                     TDFSRYILADINSDLISLYNIVKMRTDEYVQAARELFVPETNCAEVYYQFREEFNKSQ
                     DPFRRAVLFLYLNRYGYNGLCRYNLRGEFNVPFGRYKKPYFPEAELYHFAEKAQNAFF
                     YCESYADSMARADDASVVYCDPPYAPLSATANFTAYHTNSFTLEQQAHLAEIAEGLVD
                     RHIPVLISNHDTMLTREWYQRAKLHVVKVRRSISSNGGTRKKVDELLALYKPGVVSPA
                     KK"
     gene            complement(11208..12494)
                     /gene="damX"
                     /locus_tag="J1Y83_000204"
     CDS             complement(11208..12494)
                     /gene="damX"
                     /locus_tag="J1Y83_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DamX"
                     /protein_id="HBA7628394.1"
                     /translation="MDEFKPEDELKPDPSDRRTGRSRQSSERSERTERGEPQINFDDI
                     ELDDTDDRRPTRAQKARNEEPEIEEEIDESEDETVDEERVERRPRKRKKAASKPASRQ
                     YMMMGVGILVLLLLIIGIGSALKAPSTSSSDQTASGEKSIDLAGNATDQANGVQPAPG
                     TTSAENTQQDVSLPPISSTPTQGQTPAATDGQQRVEVQGDLNNALTQPQNQQQLNNVA
                     VNSTLPTEPATVAPVRNGNASRDTAKTQTAERPATTRPARQQAVIEPKKPQATVKTEP
                     KPVAQTPKRTEPAAPVASTKAPAATSTPAPKETATTAPVQTASPAQTTATPAAGGKTA
                     GNVGSLKSAPSSHYTLQLSSSSNYDNLNGWAKKENLKNYVVYETTRNGQPWYVLVSGV
                     YASKEEAKKAVSTLPADVQAKNPWAKPLRQVQADLK"
     gene            complement(12586..13674)
                     /gene="aroB"
                     /locus_tag="J1Y83_000205"
     CDS             complement(12586..13674)
                     /gene="aroB"
                     /locus_tag="J1Y83_000205"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HBA7628395.1"
                     /translation="MERIVVTLGERSYPITIASGLFNEPASFLPLKSGEQVMLVTNET
                     LAPLYLDKVRGVLEQAGVNVDSVILPDGEQYKSLAVLDTVFTALLQKPHGRDTTLVAL
                     GGGVVGDLTGFAAASYQRGVRFIQVPTTLLSQVDSSVGGKTAVNHPLGKNMIGAFYQP
                     ASVVVDLDCLKTLPPRELASGLAEVIKYGIILDGAFFNWLEENLDALLRLDGPAMAYC
                     IRRCCELKAEVVAADERETGLRALLNLGHTFGHAIEAEMGYGNWLHGEAVAAGMVMAA
                     RTSERLGQFSSAKTQRIITLLTRAGLPVNGPREMSAQAYLPHMLRDKKVLAGEMRLIL
                     PLAIGKSEVRSGVSHELVLNAIADCQSA"
     gene            complement(13731..14252)
                     /gene="aroK"
                     /locus_tag="J1Y83_000206"
     CDS             complement(13731..14252)
                     /gene="aroK"
                     /locus_tag="J1Y83_000206"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004709337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase AroK"
                     /protein_id="HBA7628396.1"
                     /translation="MAEKRNIFLVGPMGAGKSTIGRQLAQQLNMEFYDSDQEIEKRTG
                     ADVGWVFDLEGEEGFRDREEKVINELTEKQGIVLATGGGSVKSRETRNRLSARGVVVY
                     LETTIEKQLARTQRDKKRPLLHVETPPREVLEALANERNPLYEEIADVTIRTDDQSAK
                     VVANQIIHMLESN"
     gene            complement(14653..15891)
                     /gene="hofQ"
                     /locus_tag="J1Y83_000207"
                     /pseudo
     CDS             complement(14653..15891)
                     /gene="hofQ"
                     /locus_tag="J1Y83_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417850.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA uptake porin HofQ"
     gene            complement(15803..16207)
                     /gene="hofP"
                     /locus_tag="J1Y83_000208"
     CDS             complement(15803..16207)
                     /gene="hofP"
                     /locus_tag="J1Y83_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417851.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofP"
                     /protein_id="HBA7628397.1"
                     /translation="MRVKRWLLAGIALCLLTGMRDPFKPPEDLCRISELSQWHYQGMV
                     GRGERIIGVIKDGQKKWRRVQQNDVLENGWTTLQLTPDALTLGTGTNCEPPHWLWQRQ
                     GDTNEAMDSRTTVDAYTRRTGGKAAKSDADGG"
     gene            complement(16197..16637)
                     /locus_tag="J1Y83_000209"
     CDS             complement(16197..16637)
                     /locus_tag="J1Y83_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofO"
                     /protein_id="HBA7628398.1"
                     /translation="MNMLFDWWFATSPRLRQFCWAVWLLVLVTLIFLSLTHHEESDAL
                     IRLRANHHQQRAALYRLVDTTPFSEEKTLPFSPLDFQLSGAQLVYWHPSAQGGELALK
                     TLWEAVPSAFTRLAERNVSVSRFLLSVEGDDLLFTLQLETPHEG"
     gene            complement(16621..17160)
                     /locus_tag="J1Y83_000210"
     CDS             complement(16621..17160)
                     /locus_tag="J1Y83_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilN domain-containing protein"
                     /protein_id="HBA7628399.1"
                     /translation="MNPPINFLPWRQQRRTAFLRFWLLMFVAPLLLAVGITLILRLTS
                     NAEARVNAVLLQAEQQLARSLQITKPRLLERQQLREQRLQRQRQRQFTRDWQSALEAL
                     AALLPEHAWLTTISWQQGTLEIKGLTTSIAALNALETSLRQDASFYLNQRGATQQDAQ
                     GRWQFEYQLTRKVSDEHAL"
     gene            complement(17160..17939)
                     /gene="hofM"
                     /locus_tag="J1Y83_000211"
     CDS             complement(17160..17939)
                     /gene="hofM"
                     /locus_tag="J1Y83_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofM"
                     /protein_id="HBA7628400.1"
                     /translation="MAFKNWQIGLHLQQQEAVAVAIVRSAKECLLHRWWRLPLENDII
                     KDGRIVDVQRLANTLLPWSRELPQRHHIMLAFPASRTLQRSFPRPSMSLGEREQMAWL
                     SGTMARELDMDPDSLRFDYSEDSLSPAYNVTAAQSKELATLLTLAERLRVHVSAITPD
                     ASALQRFLPFLPSHQQCLAWRDNEQWLWATRYSWGRKLAVGMTSAKELAAALSVDPES
                     VALCGEGGFDPWEAVSVRQPPLPPPGGDFAIALGLALGKVY"
     gene            18059..20611
                     /gene="mrcA"
                     /locus_tag="J1Y83_000212"
     CDS             18059..20611
                     /gene="mrcA"
                     /locus_tag="J1Y83_000212"
                     /EC_number="2.4.1.129"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312265.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase/peptidoglycan
                     DD-transpeptidase MrcA"
                     /protein_id="HBA7628401.1"
                     /translation="MKFVKYFLILAVCCILLGAGSIYGLYRYIEPQLPDVATLKDVRL
                     QIPMQIYSADGELIAQYGEKRRIPVTLDQIPPEMVKAFIATEDSRFYEHHGVDPVGIF
                     RAASVALFSGHASQGASTITQQLARNFFLSPERTLMRKIKEVFLAIRIEQLLTKDEIL
                     ELYLNKIYLGYRAYGVGAAAQVYFGKTVDQLTLNEMAVIAGLPKAPSTFNPLYSMDRA
                     VARRNVVLSRMLDEGYITQQQFDQTRTEAINANYHAPEIAFSAPYLSEMVRQEMYNRY
                     GESAYEDGYRIYTTITRKVQQAAQQAVRNNVLDYDMRHGYRGPANVLWKVGESAWDNN
                     KITDTLKALPTYGPLLPAAVTSANPQEATAMLADGSTVVLSMEGVRWARPYRSDTQQG
                     PTPRKVTDVLQTGQQIWVRQVGDAWWLAQVPEVNSALVSINPQNGAVMALVGGFDFNQ
                     SKFNRATQALRQVGSNIKPFLYTAAMDKGLTLASMLNDVPISRWDAGAGSDWQPKNSP
                     PQYAGPIRLRQGLGQSKNVVMVRAMRAMGVDYAAEYLQRFGFPAQNIVHTESLALGSA
                     SFTPMQVARGYAVMANGGFLVDPWFISKIENDQGGVIFEAKPKVACPECDIPVIYGDT
                     QKSNVLENNDVEDVAISREQQNVSVPMPQLEQANQALVAKTGAQEYAPHVINTPLAFL
                     IKSALNTNIFGEPGWQGTGWRAGRDLQRHDIGGKTGTTNSSKDAWFSGYGPGVVTSVW
                     IGFDDHRRNLGHTTASGAIKDQISGYEGGAKSAQPAWDAYMKAVLEGVPEQPLTPPPG
                     IVTVNIDRSTGQLANGGNSREEYFIEGTQPTQQAVHEVGTTIIDNGEAQELF"
     gene            complement(20777..21337)
                     /gene="nudE"
                     /locus_tag="J1Y83_000213"
     CDS             complement(20777..21337)
                     /gene="nudE"
                     /locus_tag="J1Y83_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP compounds hydrolase NudE"
                     /protein_id="HBA7628402.1"
                     /translation="MSKSLQKPTILNVETVARSRLFTVESVDLEFSNGVRRVYERMRP
                     TNREAVMIVPIVDDHLILIREYAVGTESYELGFSKGLIDPGESVFEAANRELKEEVGF
                     GANDLTFLKKLSMAPSYFSSKMNIVVAQDLYPESLEGDEPEPLPQVRWPLAHMMDLLE
                     APDFNEARNVSALFLVREWLKGQGRV"
     gene            21657..23792
                     /gene="igaA"
                     /locus_tag="J1Y83_000214"
     CDS             21657..23792
                     /gene="igaA"
                     /locus_tag="J1Y83_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intracellular growth attenuator protein IgaA"
                     /protein_id="HBA7628403.1"
                     /translation="MSTIVIFLAALLACSLLAGWLIKVRSRRRQLPWTNAFADAQTRK
                     LTSEERSAVENYIESLTQVLQVPGPTGASAAPISLALNAESNNVMMLTHAITRYGIST
                     DDPNKWRYYLDSVEVHLPPFWEQYINDENTVELIHTDSLPLVISLNGHTLQEYMQETR
                     GYALQPVPSTQASIRGEESEQIELLNIRKETHEEYALSRPRGLREALLIVASFMMFFF
                     CLITPDVFVPWLAGGALLLLGAGLWGLFAPPAKSSLREIHCLRGTPRRWGLFGENDQE
                     QINNISLGIIDLVYPAHWQPYIAQDLGQQTDIDIYLDRHVVRQGRYLSLHDEVKNFPL
                     QHWLRSTIIAAGSLLVLFMLLFWIPLDMPLKFTLSWMKGAQTIEATSVKQLADAGVRV
                     GDTLRISGTGMCNIRTSGTWSAKTNSPFLPFDCSQIIWNDARSLPLPESELVNKATAL
                     TEAVNRQLHPKPEDESRVSASLRSAIQKSGMVLLDDFGDIVLKTADLCSAKDDCVRLK
                     NALVNLGNSKDWDALVKRANAGKLDGVNVLLRPVSAESLDNLVATSTAPFITHETARA
                     AQSLNSPAPGGFLIVSDEGSDFVDQPWPSASLYDYPPQEQWNAFQKLAQMLMHTPFNA
                     EGIVTKIFTDANGTQHIGLHPIPDRSGLWRYLSTTLLLLTMLGSAIYNGVQAWRRYQR
                     HRTRMMKIQAYYESCLNPQLITPSESLIE"
     gene            23857..24525
                     /gene="yrfG"
                     /locus_tag="J1Y83_000215"
     CDS             23857..24525
                     /gene="yrfG"
                     /locus_tag="J1Y83_000215"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP/IMP nucleotidase"
                     /protein_id="HBA7628404.1"
                     /translation="MHINIAWQDVDTVLLDMDGTLLDLAFDNYFWQKLVPETWGAKNG
                     VTPQEAMEYMRQQYHDVQHTLNWYCLDYWSEQLGLDICAMTTEMGPRAVLREDTIPFL
                     EALKASGKQRILLTNAHPHNLAVKLEHTGLDAHLDLLLSTHTFGYPKEDQRLWHAVAE
                     ATGLKAERTLFIDDSEAILDAAAQFGIRYCLGVTNPDSGIAEKQYLRHPSLNDYRRLI
                     PSLM"
     gene            24536..24937
                     /gene="hslR"
                     /locus_tag="J1Y83_000216"
     CDS             24536..24937
                     /gene="hslR"
                     /locus_tag="J1Y83_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated heat shock protein Hsp15"
                     /protein_id="HBA7628405.1"
                     /translation="MKEKPAVEVRLDKWLWAARFYKTRALAREMIEGGKVHYNGQRSK
                     PSKIVELNATLTLRQGNDERTVIVKAITEQRRPASEAALLYEETAESVEKREKMALAR
                     KLNALTMPHPDRRPDKKERRDLLRFKHGDSE"
     gene            24962..25840
                     /gene="hslO"
                     /locus_tag="J1Y83_000217"
     CDS             24962..25840
                     /gene="hslO"
                     /locus_tag="J1Y83_000217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417860.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HBA7628406.1"
                     /translation="MPQHDQLHRYLFENFAVRGELVTVSETLQQILENHDYPQPVKNV
                     LAELLVATSLLTATLKFDGDITVQLQGDGPMNLAVINGNNNQQMRGVARVQGEIPENA
                     DLKTLVGNGYVVITITPSEGERYQGVVGLEGDTLAACLEDYFMRSEQLPTRLFIRTGD
                     VDGKPAAGGMLLQVMPAQNAQQDDFDHLATLTETIKTEELLTLPANEVLWRLYHEEEV
                     TVYDPQDVEFKCTCSRERCADALKTLPDEEVDSILAEDGEIDMHCDYCGNHYLFNAMD
                     IAEIRNNASPADPQVH"
     gene            complement(25978..27702)
                     /locus_tag="J1Y83_000218"
     CDS             complement(25978..27702)
                     /locus_tag="J1Y83_000218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4153 domain-containing protein"
                     /protein_id="HBA7628407.1"
                     /translation="MDNVELSPATRWGMIATGLLQGLVCYLLIAWLAGKNLSWIVYGV
                     PVTVAFSSVLLFSVISFKQKRLWEWLALVFIATLGMSGWLKWQTDGMTPWRAEKALWD
                     FGCYLLLMAMLLLPWIQQSLRIRNDSSRYRYFYQSVWHNVLILLVIFLANGLTWLVLL
                     LWSELFKLVGITFFKTLFFATDWFIYLTLGLVTALAVILARTQSRLIDSIQKLFTLIA
                     TGLLPLVSLLTLMFIITLPFTGLSAISRHISAAGLLLTLAFLQLILMAIVRDPQKASL
                     PWTGPLRCLIKTALLVAPLYVFVAAWALWLRVAQYGWTADRLQGVLAVLVLLVWSLGY
                     FVSIVWRKGQNPLDLQGKVNLVVSLLVLVILVLLNSPVLDSMRISVNSHMARYQSGKN
                     TPDQVSLYMLEQSGRYGRAALESLKSDAEYMKDPKRRRGLLMAFDGVQRLQQQISEKA
                     LADNVFIASGSGKPDTIFWSAVMKNRYNAMICIEKDACVLVEQDLNSDGRAERILFAF
                     NDERYIVYGIDPAGKEWKEITMNSLPAVITKEKLLMAAEDGKLGTKPKVWRDLTVDGE
                     RLDLNLNE"
     gene            28081..29703
                     /gene="pckA"
                     /locus_tag="J1Y83_000219"
     CDS             28081..29703
                     /gene="pckA"
                     /locus_tag="J1Y83_000219"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HBA7628408.1"
                     /translation="MRVNNGLTPQELEAYGISDVHDIVYNPSYDLLYQEELDPSLTGY
                     ERGVLTNLGAVAVDTGIFTGRSPKDKYIVRDDTTRDTFWWADKGKGKNDNKPLSPETW
                     QHLKGLVTRQLSGKRLFVVDAFCGANPDTRLSVRFITEVAWQAHFVKNMFIRPSDEEL
                     AGFKPDFIVMNGAKCTNPQWKEQGLNSENFVAFNLTERMQLIGGTWYGGEMKKGMFSM
                     MNYLLPLKGIASMHCSANVGEKGDVAVFFGLSGTGKTTLSTDPKRRLIGDDEHGWDDD
                     GVFNFEGGCYAKTIKLSKEAEPEIYNAIRRDALLENVTVHEDGTIDFDDGSKTENTRV
                     SYPIYHIDNIVKPVSKAGHATKVIFLTADAFGVLPPVSRLTADQTQYHFLSGFTAKLA
                     GTERGITEPTPTFSACFGAAFLSLHPTQYAEVLVKRMQAAGAQAYLVNTGWNGTGKRI
                     SIKDTRAIIDAILNGSLDNAETFTLPMFNLAIPTELPGVDTKILDPRNTYASPEQWQE
                     KAETLAKLFIDNFDKYTDTPAGAALVAAGPKL"
     gene            complement(29779..31131)
                     /gene="envZ"
                     /locus_tag="J1Y83_000220"
     CDS             complement(29779..31131)
                     /gene="envZ"
                     /locus_tag="J1Y83_000220"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     EnvZ"
                     /protein_id="HBA7628409.1"
                     /translation="MRRLRFSPRSSFARTLLLIVTLLFVSLVTTYLVVLNFAILPSLQ
                     QFNKVLAYEVRMLMTDKLQLEDGTQLVVPPAFRREIYRELGISLYSNEAAEEAGLRWA
                     QHYEFLSHQMAQQLGGPTEVRVEVNKSSPVVWLKTWLSPNIWVRVPLTEIHQGDFSPL
                     FRYTLAIMLLAIGGAWLFIRIQNRPLVDLEHAALQVGKGIIPPPLREYGASEVRSVTR
                     AFNHMAAGVKQLADDRTLLMAGVSHDLRTPLTRIRLATEMMSEQDGYLAESINKDIEE
                     CNAIIEQFIDYLRTGQEMPMEMADLNAVLGEVIAAESGYEREIETALYPGSIEVKMHP
                     LSIKRAVANMVVNAARYGNGWIKVSSGTEPNRAWFQVEDDGPGIAPEQRKHLFQPFVR
                     GDSARTISGTGLGLAIVQRIVDNHNGMLELGTSERGGLSIRAWLPVPVTRAQGMTKEG
                     "
     gene            complement(31128..31847)
                     /gene="ompR"
                     /locus_tag="J1Y83_000221"
     CDS             complement(31128..31847)
                     /gene="ompR"
                     /locus_tag="J1Y83_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018150685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator OmpR"
                     /protein_id="HBA7628410.1"
                     /translation="MQENYKILVVDDDMRLRALLERYLTEQGFQVRSVANAEQMDRLL
                     TRESFHLMVLDLMLPGEDGLSICRRLRSQSNPMPIIMVTAKGEEVDRIVGLEIGADDY
                     IPKPFNPRELLARIRAVLRRQANELPGAPSQEEAVIAFGKFKLNLGTREMFREDEPMP
                     LTSGEFAVLKALVSHPREPLSRDKLMNLARGREYSAMERSIDVQISRLRRMVEEDPAH
                     PRYIQTVWGLGYVFVPDGSKA"
     gene            32039..32551
                     /gene="greB"
                     /locus_tag="J1Y83_000222"
     CDS             32039..32551
                     /gene="greB"
                     /locus_tag="J1Y83_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709179.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreB"
                     /protein_id="HBA7628411.1"
                     /translation="MRIIKQTKGINEMKTPLVTREGYEKLKQELNYLWREERPEVTKK
                     VTWAASLGDRSENADYQYNKKRLREIDRRVRYLTKCMENLKIVDYSPQQEGKVFFGAW
                     VEIENDDGVTHRFRIVGYDEIFGRKDYISIDSPMARALLKKEVGDLAVVNTPAGEASW
                     YVNAIEYVKP"
     gene            32648..34969
                     /locus_tag="J1Y83_000223"
     CDS             32648..34969
                     /locus_tag="J1Y83_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding transcriptional accessory protein"
                     /protein_id="HBA7628412.1"
                     /translation="MMNDSFCRIIAGEIQARPEQVDAAVRLLDEGNTVPFIARYRKEI
                     TGGLDDTQLRNLETRLSYLRELEERRQAILKSISEQGKLTDDLANAINATLSKTELED
                     LYLPYKPKRRTRGQIAIEAGLEPLADLLWSDPSHTPEVAAAQYIDADKGVADTKAALD
                     GARYILMERFAEDAALLAKVRDYLWKNAHLVSTVVSGKEEEGAKFRDYFDHHEPLSTV
                     PSHRALAMFRGRNEGILQLSLNADPQFDEPPKESYCEQIIMDHLGLRLNNAPADSWRK
                     GVVSWTWRIKVLMHLETELMGTVRERAEDEAINVFARNLHDLLMAAPAGLRATMGLDP
                     GLRTGVKVAVVDATGKLVATDTIYPHTGQAAKAAMTVAALCEKHNVELVAIGNGTASR
                     ETERFYLDVQKQFPKVTAQKVIVSEAGASVYSASELAAQEFPDLDVSLRGAVSIARRL
                     QDPLAELVKIDPKSIGVGQYQHDVSQTQLARKLDAVVEDCVNAVGVDLNTASVPLLTR
                     VAGLTRMMAQNIVAWRDENGQFQNRQQLLKVSRLGPKAFEQCAGFLRINHGDNPLDAS
                     TVHPEAYPVVERILAATQQALKDLMGNSSELRNLKASDFTDEKFGVPTVTDIIKELEK
                     PGRDPRPEFKTAQFADGVETMNDLQPGMILEGAVTNVTNFGAFVDIGVHQDGLVHISS
                     LSNKFVEDPHTVVKAGDIVKVKVLEVDLQRKRIALTMRLDEQPGETNARRGGGNERLQ
                     NNRPAAKPRGREAQPAGNSAMMDALAAAMGKKR"
     gene            35407..35634
                     /gene="feoA"
                     /locus_tag="J1Y83_000224"
     CDS             35407..35634
                     /gene="feoA"
                     /locus_tag="J1Y83_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transporter A"
                     /protein_id="HBA7628413.1"
                     /translation="MQYTPDTAWKITGFSREISPAYRQKLLSLGMLPGSSFNVVHVAP
                     LGDPIHIETRRVSLVLRKKDLALLEVEAVSC"
     gene            35651..37972
                     /gene="feoB"
                     /locus_tag="J1Y83_000225"
     CDS             35651..37972
                     /gene="feoB"
                     /locus_tag="J1Y83_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(2+) transporter permease subunit FeoB"
                     /protein_id="HBA7628414.1"
                     /translation="MKKLTIGLIGNPNSGKTTLFNQLTGSRQRVGNWAGVTVERKEGQ
                     FSTTDHQVTLVDLPGTYSLTTISSQTSLDEQIACHYILSGDADLLINVVDASNLERNL
                     YLTLQLLELGIPCIVALNMLDIAEKQNIRIEIDALSARLGCPVIPLVSTRGRGIEALK
                     LAIDRYKANENVELVHYAQPLLNEADSLAKVMPSDIPLKQRRWLGLQMLEGDIYSRAY
                     AGEASQHLDAALARLRNEMDDPALHIADARYQCISAICDVVSNTLTAEPSRFTTAVDK
                     IVLNRFLGLPIFLFVMYLMFLLAINIGGALQPLFDVGSVALFVHGIQWIGYTLHFPDW
                     LTIFLAQGLGGGINTVLPLVPQIGMMYLFLSFLEDSGYMARAAFVMDRLMQALGLPGK
                     SFVPLIVGFGCNVPSVMGARTLDAPRERLMTIMMAPFMSCGARLAIFAVFAAAFFGQN
                     GALAVFSLYMLGIVMAVLTGLMLKYTIMRGEATPFVMELPVYHVPHVKSLIIQTWQRL
                     KGFVLRAGKVIIIVSIFLSAFNSFSLSGKIVDNINDSALASVSRVITPVFKPIGVHED
                     NWQATVGLFTGAMAKEVVVGTLNTLYTAENIQDEEFNPAEFNLGEELFSAVDETWQSL
                     KDTFSLSVLMNPIEASKGDGEMGTGAMGVMDQKFGSAAAAYSYLIFVLLYVPCISVMG
                     AIARESSRGWMGFSILWGLNIAYSLATLFYQVASYSQHPTYSLVCILAVILFNIVVIG
                     LLRRARSRVDVELLATRKSVSSCCAASTTGDCH"
     gene            37983..38219
                     /gene="feoC"
                     /locus_tag="J1Y83_000226"
     CDS             37983..38219
                     /gene="feoC"
                     /locus_tag="J1Y83_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[Fe-S]-dependent transcriptional repressor FeoC"
                     /protein_id="HBA7628415.1"
                     /translation="MASLIQVRDLLALRGRMEEAQISQTLNTPQPMINAMLKQLESMG
                     KAVRIQEEPDGCLSGSCKSCPEGKACLHEWWTLR"
     gene            38421..39302
                     /gene="rpnA"
                     /locus_tag="J1Y83_000227"
     CDS             38421..39302
                     /gene="rpnA"
                     /locus_tag="J1Y83_000227"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000039101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnA"
                     /protein_id="HBA7628416.1"
                     /translation="MSKKQSSTPHDALFKLFLRQPETARDFLAFHLPAPIHALCDMKT
                     LKLESSSFIDDDLRESYSDVLWSVKTEQGPGYIYCLIEHQSTSNKLIAFRMMRYAIAA
                     MQNHLDAGYKTLPMVVPLLFYHGIESPYPYSLCWLDCFADPKLARQLYASAFPLIDVT
                     VMPDDEIMQHRRMALLELIQKHIRQRDLMGLVEQMACLLSSGYANDRQIKGLFNYIRQ
                     TGDAVRFNDFIDGVAERSPKHKESLMTIAERLRQEGFQKGIRAIAKTMLDAGVSLEDV
                     LRFTSLTVEDLAEINHQ"
     gene            complement(39522..40292)
                     /gene="bioH"
                     /locus_tag="J1Y83_000228"
     CDS             complement(39522..40292)
                     /gene="bioH"
                     /locus_tag="J1Y83_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pimeloyl-ACP methyl ester esterase BioH"
                     /protein_id="HBA7628417.1"
                     /translation="MNNIWWQTKGQGNVHLVLLHGWGLNAEVWRCIDEELSSHFTLHL
                     VDLPGFGRSRGFGALSLADMAEVVLRQAPDKAIWLGWSLGGLVASQIALTHPERVQAL
                     VTVASSPCFSARDEWPGIKPDVLAGFQQQLSDDFQRTVERFLALQTMGTETARQDARA
                     LKKTVLALPMPEVDVLNGGLEILKTVDLRQPLQNVPMPFLRLYGYLDGLVPRKVVPML
                     DKLWPHSESYIFAKAAHAPFISHPDEFCHLLVALKQRV"
     gene            40330..41013
                     /gene="gntX"
                     /locus_tag="J1Y83_000229"
     CDS             40330..41013
                     /gene="gntX"
                     /locus_tag="J1Y83_000229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417872.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein GntX"
                     /protein_id="HBA7628418.1"
                     /translation="MLTVPGLCWLCRMPLALGHWGICSVCSRAARTDKTLCPQCGLPA
                     THSHLPCGRCLQKPLPWQRLVTVADYAPPLSPLIHQLKFSRRSEIASALSRLLLLEVL
                     HARRTTGLQLPDRIISVPLWQRRHWRRGFNQSDLLCQPLSRWLHCQWDSEAVTRTRAT
                     ATQHFLNARLRKHNLKNAFRLELPVQGRHMVIVDDVVTTGSTVAEIAQLLLRNGAATV
                     QVWCLCRTL"
     gene            41072..41647
                     /gene="nfuA"
                     /locus_tag="J1Y83_000230"
     CDS             41072..41647
                     /gene="nfuA"
                     /locus_tag="J1Y83_000230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016942932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S biogenesis protein NfuA"
                     /protein_id="HBA7628419.1"
                     /translation="MIRISDAAQAHFAKLLANQEEGTQIRVFVINPGTPNAECGVSYC
                     PPDAVEATDTALKFDLLTAYVDELSAPYLEDAEIDFVTDQLGSQLTLKAPNAKMRKVA
                     DDAPLMERVEYMLQSQINPQLAGHGGRVSLMEITEDGYAILQFGGGCNGCSMVDVTLK
                     EGIEKQLLNEFPELKGVRDLTEHQRGEHSYY"
     gene            42011..43327
                     /gene="gntT"
                     /locus_tag="J1Y83_000231"
     CDS             42011..43327
                     /gene="gntT"
                     /locus_tag="J1Y83_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate transporter"
                     /protein_id="HBA7628420.1"
                     /translation="MPLVIVAIGVILLLLLMIRFKMNGFIALVLVALAVGLMQGMPLD
                     KVIGSIKAGVGGTLGSLALIMGFGAMLGKMLADCGGAQRIATTLIAKFGKKHIQWAVV
                     LTGFTVGFALFYEVGFVLMLPLVFTIAASANIPLLYVGVPMAAALSVTHGFLPPHPGP
                     TAIATIFNADMGKTLLYGTILAIPTVILAGPVYARVLKGIDKPIPEGLYSAKTFSEEE
                     MPSFGVSVWTSLVPVVLMAMRAIAEMILPKGHAFLPVAEFLGDPVMATLIAVLIAMFT
                     FGLNRGRSMDQINDTLVSSIKIIAMMLLIIGGGGAFKQVLVDSGVDKYIASMMHETNI
                     SPLLMAWSIAAVLRIALGSATVAAITAGGIAAPLIATTGVSPELMVIAVGSGSVIFSH
                     VNDPGFWLFKEYFNLTIGETIKSWSMLETIISVCGLVGCLLLNMVI"
     gene            complement(43437..45521)
                     /gene="malQ"
                     /locus_tag="J1Y83_000232"
     CDS             complement(43437..45521)
                     /gene="malQ"
                     /locus_tag="J1Y83_000232"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HBA7628421.1"
                     /translation="MESKRLDNAALAAGISPNYINAHGKPQSISAETKRRLLDAMHQR
                     TATKVAVTPVPNVMVYTSGKKMPMVVEGSGEYSWLLTTEEGKQYKGHVTGGKAFNLPT
                     KLPEGYHTLTLTQDDQRAHCRVIVAPKRCYEPQALLNKQKLWGACVQLYTLRSEKNWG
                     IGDFGDLKAMLVDVAKRGGSFIGLNPIHALYLANPESASPYSPSSRRWLNVIYIDVNA
                     VEDFHLSEEAQAWWQLPTTQQTLQQARDADWVDYSTVTALKMTALRMAWKGFAQRDDE
                     QMTAFRQFVAEQGDSLFWQAAFDALHAQQVKEDEMRWGWPAWPEMYQNVDSPEVRQFC
                     EEHRDDVDFYLWLQWLAYSQFAACWEISQGYEMPIGLYRDLAVGVAEGGAETWCDREL
                     YCLKASVGAPPDILGPLGQNWGLPPMDPHIITARAYEPFIELLRANMQNCGALRIDHV
                     MSMLRLWWIPYGETADQGAYVHYPVDDLLSILALESKRHRCMVIGEDLGTVPVEIVGK
                     LRSSGVYSYKVLYFENDHEKTFRAPKAYPEQSMAVAATHDLPTLRGYWESGDLTLGKT
                     LGLYPDEVVLRGLYQDRELAKQGLLDALHKYGCLPKRAGHKASLMSMTPTLNRGLQRY
                     IADSNSALLGLQPEDWLDMADPVNIPGTSYQYKNWRRKLSATLESMFADDGVNKLLKD
                     LDRRRRAAAKKK"
     gene            complement(45531..47924)
                     /gene="malP"
                     /locus_tag="J1Y83_000233"
     CDS             complement(45531..47924)
                     /gene="malP"
                     /locus_tag="J1Y83_000233"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrin phosphorylase"
                     /protein_id="HBA7628422.1"
                     /translation="MSQPIFNDKQFQEALSRQWQRYGLNSAAEMTPRQWWLAVSEALA
                     EMLRAQPFAKPVANQRHVNYISMEFLIGRLTGNNLLNLGWYQDVQDSLKAYDINLTDL
                     LEEEIDPALGNGGLGRLAACFLDSMATVGQSATGYGLNYQYGLFRQSFVDGKQVEAPD
                     DWHRSNYPWFRHNEALDVQVGIGGKVTKDGRWEPEFTITGQAWDLPVVGYRNGVAQPL
                     RLWQATHAHPFDLTKFNDGDFLRAEQQGINAEKLTKVLYPNDNHTAGKKLRLMQQYFQ
                     CACSVADILRRHHLAGRKLHELADYEVIQLNDTHPTIAIPELLRVLIDEHQMSWDDAW
                     AITSKTFAYTNHTLMPEALERWDVKLVKGILPRHMQIINEINTRFKTLVEKTWPGDEK
                     VWAKLAVVHDKQVHMANLCVVGGFAVNGVAALHSDLVVKDLFPEYHQLWPNKFHNVTN
                     GITPRRWIKQCNPALAALLDKSLKKEWANDLDQLINLEKFADDAKFRQQYREIKQANK
                     VRLAEFVKVRTGIEINPQAIFDIQIKRLHEYKRQHLNLLHILALYKEIRENPQSDRVP
                     RVFLFGAKAAPGYYLAKNIIFAINKVADVINNDPLVGDKLKVVFLPDYCVSAAEKLIP
                     AADISEQISTAGKEASGTGNMKLALNGALTVGTLDGANVEIAEKVGEENIFIFGHTVE
                     QVKAILAKGYDPVKWRKKDKVLDAVLKELESGKYSDGDKHAFDQMLHSIGKQGGDPYL
                     VMADFAAYVEAQKQVDVLYRDQEAWTRAAILNTARCGMFSSDRSIRDYQARIWQAKR"
     gene            48547..51252
                     /gene="malT"
                     /locus_tag="J1Y83_000234"
     CDS             48547..51252
                     /gene="malT"
                     /locus_tag="J1Y83_000234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000907024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MalT"
                     /protein_id="HBA7628423.1"
                     /translation="MLIPSKLSRPVRLDHTVVRERLLAKLSGANNFRLALITSPAGYG
                     KTTLISQWAAGKNDIGWYSLDEGDNQQERFASYLIAAMQQATNGHCAICETMAQKRQY
                     ASLTSLFAQLFIELAEWHSPLYLVIDDYHLITNPVIHESMRFFIRHQPENLTLVVLSR
                     NLPQLGIANLRVRDQLLEIGSQQLAFTHQEAKQFFDCRLSSPIEAAESSRICDDVSGW
                     ATALQLIALSARQNTHSAHKSARRLAGINASHLSDYLVDEVLDNVDLATRHFLLKSAI
                     LRSMNDALITRVTGEENGQMRLEEIERQGLFLQRMDDTGEWFCYHPLFGNFLRQRCQW
                     ELAAELPEIHRAAAESWMAQGFPSEAIHHALAAGDALMLRDILLNHAWSLFNHSELSL
                     LEESLKALPWDSLLENPQLVLLQAWLMQSQHRYGEVNTLLARAEHEIKDIREGTMHAE
                     FNALRAQVAINDGSPDEAERLAKLALEELPPGWFYSRIVATSVLGEVLHCKGELTRSL
                     ALMQQTEQMARQHDVWHYALWSLIQQSEILFAQGFLQTAWETQEKAFQLINEQHLEQL
                     PMHEFLVRIRAQLLWAWARLDEAEASARSGIEVLSSYQPQQQLQCLAMLIQCSLARGD
                     LDNARSQLNRLENLLGNGKYHSDWISNANKVRVIYWQMTGDKAAAANWLRHTAKPEFA
                     NNHFLQGQWRNIARAQILLGEFESAEIVLEELNENARSLRLMSDLNRNLLLLNQLYWQ
                     AGRKSDAQRVLLDALKLANRTGFISHFVIEGEAMAQQLRQLIQLNTLPELEQHRAQRI
                     LREINQHHRHKFAHFDENFVERLLNHPEVPELIRTSPLTQREWQVLGLIYSGYSNEQI
                     AGELEVAATTIKTHIRNLYQKLGVAHRQAAVQHAQKLLKMMGYGV"
     gene            complement(51295..52311)
                     /gene="rtcA"
                     /locus_tag="J1Y83_000235"
     CDS             complement(51295..52311)
                     /gene="rtcA"
                     /locus_tag="J1Y83_000235"
                     /EC_number="6.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 3'-terminal phosphate cyclase"
                     /protein_id="HBA7628424.1"
                     /translation="MKRMIALDGAQGEGGGQILRSALSLSMITGQPFTITGIRAGRAK
                     PGLLRQHLTAVKAAAEICRATVEGAELGSQRLVFRPGTVRGGDYRFAIGSAGSCTLVL
                     QTVLPALWFADGPSRVEVSGGTDNPSAPPADFIRRVLEPLLAKIGVHQQTTLLRHGFY
                     PAGGGVVATEVSPVASFNSLQLGERGNIVQMRGEVLLAGVPRHVAEREIATLAGSFSL
                     HEQNIHNLPRDQGPGNTVSLEVESENITERFFVVGEKRVSAEVVAAQLVKEVKRYLAS
                     PAAVGEYLADQLVLPMALAGAGEFTVAHPSCHLQTNIAVVERFLPVRFSLIETDGVTR
                     VSIE"
     gene            complement(52315..53541)
                     /locus_tag="J1Y83_000236"
     CDS             complement(52315..53541)
                     /locus_tag="J1Y83_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RtcB family protein"
                     /protein_id="HBA7628425.1"
                     /translation="MNYELLTTENAPVKMWTKGVPVEADARQQLINTAKMPFIFKHIA
                     VMPDVHLGKGSTIGSVIPTKGAIIPAAVGVDIGCGMNALRTALTAADLPENLAELRQA
                     IETAVPHGRTTGRCKRDKGAWENPPVNVDAKWAELEAGYQWLTQKYPRFLNTNNYKHL
                     GTLGTGNHFIEICLDESDQVWIMLHSGSRGIGNAIGTYFIDLAQKEMQETLETLPSRD
                     LAYFMEGTEYFDDYLKAVAWAQLFASLNRDAMMENVVTALQSVTQKTVKQPQTLAMEE
                     INCHHNYVQKEQHFGEEIYVTRKGAVSARAGQYGIIPGSMGAKSFIVRGLGNEESFCS
                     CSHGAGRVMSRTKAKKLFSVEDQIRATAHVECRKDAEVIDEIPMAYKDIDAVMAAQSD
                     LVEVIYTLRQVVCVKG"
     gene            53730..55328
                     /locus_tag="J1Y83_000237"
     CDS             53730..55328
                     /locus_tag="J1Y83_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-dependent transcriptional regulator"
                     /protein_id="HBA7628426.1"
                     /translation="MRKTVAFGFVGTVLDYAGRGSQRWSKWRPTLCLCQQESLVVDRL
                     ELLHDARSRSLFETLKRDIASVSPETKVVGVEIELHNPWDFEEVYACLHDFARGYAFQ
                     PDKEEYLIHITTGTHVAQICWFLLAEARYLPTRLIQSSPPRKKERPDSPGAVTIIDLD
                     LSRYNAIASRFAEERQQTLDFLKSGIATRNSHFNRMIEQIEKVAIKSRAPILLNGPTG
                     AGKSFLARRIFELKQARHQFSGAFVEVNCATLRGDTAMSTLFGHVKGAFTGARESREG
                     LLRSANGGMLFLDEIGELGADEQAMLLKAIEEKTFYPFGSDRQVSSDFQLIAGTVRDL
                     RQLVAEGKFREDLYARINLWTFTLPGLRQRQEDIEPNLDYEVERHASLTGDSVRFNTE
                     ARRAWLAFATSPQATWRGNFRELSASVTRMATFATSGRITLDTVEDEINRLRYNWQES
                     RPSTLTALLGAEAENIDLFDRMQLEHVIAICRQAKSLSAAGRELFDISRQGKASVNDA
                     DRLRKYLARFGLTWETMQDQHSSS"
     gene            complement(55310..56068)
                     /locus_tag="J1Y83_000238"
     CDS             complement(55310..56068)
                     /locus_tag="J1Y83_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000815090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family transcriptional regulator"
                     /protein_id="HBA7628427.1"
                     /translation="MKQTQRHNGIIELVKQQGYVSTEELVEHFSVSPQTIRRDLNELA
                     EQNLILRHHGGAALPSSSVNTPWHDRKATQTEEKERIARKVAEQIPNGSTLFIDIGTT
                     PEAVAHALLNHSNLRIVTNNLNVANTLMVKEDFRIILAGGELRSRDGGIIGEATLDFI
                     SQFRLDFGILGISGIDSDGSLLEFDYHEVRTKRAIIENSRHVMLVVDHSKFGRNAMVN
                     MGSISMVDAVYTDTMPPASVMQVLKDHHIQLELC"
     gene            complement(56085..56915)
                     /gene="glpG"
                     /locus_tag="J1Y83_000239"
     CDS             complement(56085..56915)
                     /gene="glpG"
                     /locus_tag="J1Y83_000239"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease
                     GlpG"
                     /protein_id="HBA7628428.1"
                     /translation="MLMITSFANPRVAQAFVDYMATQGVILTIQQHNQSDVWLADESQ
                     AERVRAELARFLENPADPRYLAASWQAGHTGSGLHYRRYPFFAALRERAGPVTWVMMI
                     ACVVVFIAMQILGDQEVMLWLAWPFDPTLKFEFWRYFTHALMHFSLMHILFNLLWWWY
                     LGGAVEKRLGSGKLIVITLISALLSGYVQQKFSGPWFGGLSGVVYALMGYVWLRGERD
                     PQSGIYLQRGLIIFALIWIVAGWFDLFGMSMANGAHIAGLAVGLAMAFVDSLNARKRK
                     "
     gene            complement(56960..57286)
                     /gene="glpE"
                     /locus_tag="J1Y83_000240"
     CDS             complement(56960..57286)
                     /gene="glpE"
                     /locus_tag="J1Y83_000240"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase GlpE"
                     /protein_id="HBA7628429.1"
                     /translation="MDQFECINVTDAHQKLQEKEAVLVDIRDPQSFAMGHATQAFHLT
                     NDTLGAFMRDNDFDTPVMVMCYHGNSSKGAAQYLLQQGYDVVYSIDGGFEAWQRQFPA
                     EVAYGA"
     gene            57476..58981
                     /gene="glpD"
                     /locus_tag="J1Y83_000241"
     CDS             57476..58981
                     /gene="glpD"
                     /locus_tag="J1Y83_000241"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase"
                     /protein_id="HBA7628430.1"
                     /translation="METKDLIVIGGGINGAGIAADAAGRGLSVLMLEAQDLACATSSA
                     SSKLIHGGLRYLEHYEFRLVSEALAEREVLLKMAPHIAFPMRFRLPHRPHLRPAWMIR
                     IGLFMYDHLGKRTSLPGSTGLRFGANSVLKPEIKRGFEYSDCWVDDARLVLANAQMVV
                     RKGGEVLTRTRATSARRENGLWIVEAEDIDTGKKYTWQARGLVNATGPWVKQFFDDGM
                     HLPSPYGIRLIKGSHIVVPRVHTQKQAYILQNEDKRIVFVIPWMDEFSIIGTTDVEYK
                     GDPKAVKIEESEINYLLKVYNTHFKKQLSRDDIIWTYSGVRPLCDDESDSPQAITRDY
                     TLDIHDEDGKAPLLSVFGGKLTTYRKLAEHALEKLTPYYQGIGPAWTKESVLPGGAIE
                     GDRDDYAARLRRRYPFLTESLARHYARTYGSNSELLLGNAGAISDLGEDFGHEFYEAE
                     LKYLVDHEWVRRADDALWRRTKQGMWLNADQQSRVSQWLMEYTQQRLSLAS"
     gene            complement(59035..59640)
                     /locus_tag="J1Y83_000242"
     CDS             complement(59035..59640)
                     /locus_tag="J1Y83_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000589351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA7628431.1"
                     /translation="MNKFRLYIYPVIFGFVIVPLLVWPAVIALAVLIFTLTFLAEIIF
                     SFPLLVVRISLQELQLELLVEYALFFSVMCGIGWQFSRRTPPELKNRLHCWLVFSPIY
                     FWLILSNFILYISPEKSALLENIRNFFLTFVWLPLNFSPFWPQPWTDFVGPISAQLGF
                     ALGYYCQWRSKNRSHRKKWGDWVTCLSLAILALGPLFNYLQ"
     gene            complement(59643..60419)
                     /locus_tag="J1Y83_000243"
     CDS             complement(59643..60419)
                     /locus_tag="J1Y83_000243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000553741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA7628432.1"
                     /translation="MQPSPAMQALIEQVYHIFRRYPVPQQFVVCCEYCLSQQEQKALR
                     NTSLRAIPYSLINAWNSSPGPDPQNSDEVRYFLPRLLEFVAQRQFDNIHEVFSLRRIN
                     LASKENWREDERAILQRFACQYMADWVSGDEAVELQYMLEMFSRADIALSPLLDAVIS
                     VPGYQPTVSMACLLWHNREENIQNSRFEQDDDDKAITAQINAWAFNNQSILKERARQA
                     IENPLKQPEQGTQYQIWEDDWMIDECLCAMYDASSESSGQ"
     gene            complement(60385..61131)
                     /locus_tag="J1Y83_000244"
     CDS             complement(60385..61131)
                     /locus_tag="J1Y83_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000424554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA7628433.1"
                     /translation="MELVDTLFASLSGTEPFTGVDITIANCKSTYWDEGIVQQLINQV
                     LDEGEKFAGAAGLEGLSRYDVTLNIGLTSSNVWPGFSLDTATISRLCACGADFGFDLY
                     ISDVPDVQCDLNTTNDFTVQFTAMLNPDERVIIAKRPLKKCDAWIEDVYIFQVFKEAW
                     QFQNGNSLRGFRDKQAELKLYARHYSVENCTEESCWDCNYCIRPSFSLSRSAIIRLNA
                     ANARFVYQPFTRDQRARGNYATQPRHAGIN"
     gene            complement(61305..62255)
                     /locus_tag="J1Y83_000245"
     CDS             complement(61305..62255)
                     /locus_tag="J1Y83_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001327556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HBA7628434.1"
                     /translation="MVSDVNKNAPGTVLPSQIWKVGTYKAYCNSLDDYEIYFSAVSGI
                     DPSGASGDHQGSDVFIPLTHEISVSTHIKLYNQNGTMTDKIVPFENYNTNYPGDRSKP
                     SNWASDTEGYIKIRIDKKIISDVSLSNVLLVSLYVSQIPTEHGPIPVFNAYIGNLNIQ
                     VPQGCTINEGTSFTVNMPDVWASELSRAGAGAKPAGVTPVATTIPINCTNKDTDAVMT
                     LVFDGNISATRDTNGKQSIIQAQDNPDVGIMIMDSQQNSVDLNALATSVGVPFRLVEN
                     QAASAQTADVTFLTLPVSTTGKVPAAGRYNALAILRVEYQ"
BASE COUNT        15139 a        16939 c        15522 g        14794 t
ORIGIN      
        1 ctggtaggcg cagtactggc tacgttaact gcatgtactg gtcatattga aaaccgtgat
       61 aagaactgct cttacgacta cctgctgcac ccggctattt ctatttctaa aatcattggc
      121 ggttgcggtc cgactgcaca gtaagtctct gacagaaatc ggctaacgcc ggtttaattg
      181 cctgacgccc tcggagttat ccgagggcgt atttgtttct gcattctttt caaaaatatc
      241 gtttacaacg attaaatgct ggcaatactt tcacgcaaga tccgcaatat cgtctcttca
      301 ccgctgcgat ttgggttaat gcgaatcgcg caatgttcta actgcggatt cgcctggcga
      361 aacgttccgg aaagtcgata aaagagcggc gggatttcat attttgactc tgcaccaacc
      421 gggtaaggca aggcaccgag tttctgcgcc tcttccagca ctctggcggc aatcggctga
      481 tgaaactcca caatcaatac cttcgactgt gcattggcaa tgacggcgct tttcacttcc
      541 gccaccgcgc cgccgttaag caaagccagc aaccgttcag atactcccgc ctgcaccgca
      601 tgcatcaccg gcgcaaacac cagcccacgc aagacttcca gcgcctgtgc accctgaatc
      661 tggctgccgc cggaatagag cgtggcgcga atccggctaa taacatcggc atcgcctacc
      721 accgcaccaa cgccctctgg cccaaacagc ttgaagcagg agaatgtcga cacattcgcc
      781 ccgcattcgc agccaattcg cgacaccttc atcaccgcat agttgtcatc ggttaacgct
      841 ggaacacctg ccgcgcgcaa cgttgccagc acgtctgcca gcacatagcg gtcctgcggc
      901 tgctggcgcg tatgctgcac cagcgcggca tcaggttgtt gctcgtcaac aacctgcttc
      961 agtgccgaca gatcattgaa atcagccgta atgagcgtta gccccatctg ctcaataatg
     1021 acgcgcgtcg ttggataaac cggcgcgtca tgcaccagaa gacgttgccc cggtttgagc
     1081 aaagccgcca gtgccgcacg aatcgcgcca gtacccgcgc cctgtaccag cgccgccgcg
     1141 ggtgcatgaa acgcgtcagc cagcacctgt tccacacgct gggtaatgcg gggctgattt
     1201 agccccggcg ttaagcccaa atcaccgcag gtaagaaact cgctgccagg aaaatgacgg
     1261 catatcgtgt ccaccagcgc aaactgcttt tgctgcgcct caataagcgt caggctttgc
     1321 agaggaaacg tcttcatagg tgccttcctt aagccggaac gaacaggcca agccagtaaa
     1381 gaacattcag caggatgccg gtgatcatta ctgctacgac cggtgcagcc attttctgta
     1441 ccggacggcc cagggattcg ttgaggaagt aaatggcaac cgcgatagag aatccggtat
     1501 agcccgccat tttgattgct gcaaagatcg aaccgaccag caacgccact tccatcagca
     1561 tattcatggc gttacggatg ttatccgacg cattacgtac cgacgggtag cgccccaacc
     1621 atttgccgat cgaacgaagc agcaagactt ccgccgaaat aaccactgcg cctaataccg
     1681 ctgcaaccat cggattcggc gagagatagc ccaccgcata aacaaaggta aagcccgcaa
     1741 ctgcatacac accggtggct aacgcggtgg tggcaatcaa cggcacaaaa cccagcccgc
     1801 gcataaattc tgccagtgcc gcctggttga ttagtgtttg cgattgttcc ggcgttacgc
     1861 ctgcggagta cgctttctcc agtgtgaaga tcgacacttc actaccggcg aaaatcttca
     1921 tgctggcaac ggcagcaatc aatgcaccca cgatggcgat atacggtaag tttttgataa
     1981 tccgtgatgt acgctcttca aacaccgaca taccgctggc atcgatatca ttttcatcac
     2041 gatgacgcag gtcatgagtt attgcgatcc ccagcagcat caccatgcca ataaagattt
     2101 cgatggattc agggttaaga tgtgggaaat agcgcactac caccacacga gtcatcagta
     2161 ccaccacggc ggcgatcaga ctttgcttcc agccaaactg gtagaaaatc gccaccaacg
     2221 ggaacagtgc aaaagctgaa accaccggcg agcttaattc ccccaggcta cctaatacat
     2281 ccaccggtag cgcggtcagc agctggttta ctggcaacag acaggtgagg atcaatattc
     2341 cccagatagc accaagacca aacgccatca ggctgtttat cgccagtacg ccgaggatat
     2401 cggtaggaag aaagagtaac catgcgttga gcagcccggt tttcagggta aaagagatcc
     2461 ccaccgaggc cacaaaacca atgctcagac caaaagcgat actccccgcc tcgcgacggt
     2521 tcatatagcc ttcaatcagt tgcggcagga tcgggcggat accgtcatga aaaacggctg
     2581 ccgagcgatg cgccagaagc gatgtcatac ccgtcaggca cgccaccacg ataatctgaa
     2641 tgtacagatc catagtcgtg ccttttattt cagatggtta atcagcatcg gaatcgcgtg
     2701 ttcgacgtgc tcaacggaaa ggccaaacgc cacttttcct tcagcgatca ttttggcgat
     2761 atgttcgtct ttcgctttaa tgcccggctt ggcaatggtg caacttttgt tatagccaat
     2821 caccgcgata gcaatcgaca atgccgcgcc cgcaccggta ttacacgcac cgatgtagta
     2881 atccagttgc ccggatttca ctttcattgc cgcttccatg tcgttgtgaa tgaaaacttc
     2941 aaaacagcca ggagccgtcg cttcaatggt ttttttaatc tgctcacgct gtaagcctgc
     3001 aacgccaatc tttttcatga gtctgtcctt attggaaaaa ttgagaggga ttttcacgta
     3061 acatcacatc cacatcggcc tgactgaatc ctgactggcg caattgcgga ataaaggtgg
     3121 tcaataaaaa gtcatagcca taaccaccgt tagcttttaa atgagagcgc cgcgtaatat
     3181 ccatcgacag catgacgcgg ttcagcaacc cacggtcacg tagcgcatgg agcatcgcaa
     3241 tgcgcttttc gtccgggtag taactgttct tgccgatggt gtcgaactgc acatacgcgc
     3301 cgagatcgat catcttcaaa atgttgtcga gattgtcttt cagatcgcag tggccgacgg
     3361 tgacgcgcga aagatcaact ccgtgggctt gcaacagagc cagttgctcc agccccatcg
     3421 tgctgaacga cgtatgcgtg gagatcgggc gtctggtctg gttatgtgcc agcgcggcgg
     3481 caataaacac cttctcttcc agcggcgtaa tcttcccttc gctggagcca atctccgcga
     3541 tgatcccggc tttcaggtca gtgccatcga taccctgctc aatttcatcg accatctcct
     3601 gcgccagttc ctgcacgctg cgggtcgcca catgttccgg gaaaaacgcg tcctggtaat
     3661 aaccggtaca ggccaccacg ttgatcccgg tttcgcgcat tacatcgagc ataaattgcg
     3721 cattgcgccc catgtaacgg ttggtcatct caatcacatt acgcacgccc cgggccatca
     3781 ggtcgttcat ctcctggcaa atgaacgcat actgatcaag gcggcagtcc acgttgtttt
     3841 taaagccgga aagatcaata tgcagatgct catgggcaag ggtgtagccc gtcggatcaa
     3901 aactcatagg gtgtctcctg caaaccggaa ggaggaaaga aaagagcgac cattttgtgg
     3961 tggtggcgca cggaaaaatt cacacacggt agcccccaca tcagaaagcg tggtgcgcac
     4021 accgagctgc gtagcgacca gtccttgctg ataaaccagc actggcacca cttcgcgggt
     4081 atggtggctg tgaccaatgg tcggatcgtt accgtgatcc gccatcacga ccaggcaatc
     4141 atctggctgc atcgcctcaa caagccgggc aaggttacgg tcaacgacct gcaaacgttc
     4201 ggcataacgt gcgacgtctt ctgcatgacc agcgaggtcg gtttcctgga tattggtaca
     4261 aataaacgcc gtcggatagg tgttaaattc gttgagggtg atatccataa tccgctggct
     4321 atccaccagg ttttgccagc tcatgccata aggattgctg acgatatctg ccaccttacc
     4381 caccagcacg gttggcacgc ccgcttcata cagtttttgc ggcacctgca ctttttcatc
     4441 gacaccgtag cccatatgta cgacctggaa accgttgtca taggcgccag aacgcggcgc
     4501 attgatgcca ataaagcgcc cttctttgct ttctgcggca tcgaggatgc actggctgtc
     4561 ggttaacagg ccaccaaatg taatgacccg accgacctgt acctgctcac gcacgatacg
     4621 acccattttg attgcgtcgt caaaagagat cacagagaga ttggcggtga tgttatagac
     4681 ctgacctaaa tccgcctcga gattatcgcc aatcgcaacc gcctgattga cccacagaaa
     4741 ttgcagatca tcgccacgac gctcaacctg ccagccagcg gaaactaatg cctgctcaac
     4801 acggtcaatc acatcatgaa agggcatccg cagcggcggt aatgggcgcg tacctaaaat
     4861 ctcctgatgc cccataaagg tatcgccacc ttcatgttgc agctctgcca cgccccaggt
     4921 tgcggagtct gacggctgca tatcgccagg cgcataaccc aatgcgttga ttagccccag
     4981 cgtctccagc gttggtagct gcaaatgcgg caactggctc aggatgtgac cacaggtatt
     5041 cgccccggca tcctgcggac gcaccaacgt gacatctttc attgccccta cgccaaagct
     5101 atcaatcact aacaccacaa atcgcgccat ttacgcctcc agagggttgc cgaggctgtc
     5161 ataacgacca actatttctg gttcgccacg gtgaataccg gacaccagca ccacatcgct
     5221 acgggtgata aaaatctgcg tgcgaaaaca gagcaccact gcactgctta ccggatgctc
     5281 gcctgccagc ggcagggtgt agtcgatact gctgtcatca acggcgttga gatatgtttc
     5341 agtaattctt tgattttctg gcgtaaacac cagcgcatgt tgcgcgtgac cacgacggta
     5401 gtaaccgccg ccgtagcagt agctgtcgcc acggaaatga tgggagattt cacttaacca
     5461 gagcatcgcg atacgttcag gctgatcgcc ctgttgattt gccggaatag tgcccgtcag
     5521 cgcatgaccg ggttcggtat gagtcacacc gtattccgcc agcaatggca gcgacgtgca
     5581 gctggtcgct gaaggcgcgt tcagttgctc aatcgcaata ccagatttcg ccagttgatc
     5641 ccgtgcttgt accagcgtgt gaagattcgg tgtcggcaaa actttcccgg cagcctcatc
     5701 ccaaagcagg caagggaaat gggtaagtcc cgccaaatgt agccccggca gattctggat
     5761 ttcagcaacc acctcgtgca gcgaatgctg tacaaaaccg ctctcctgcc ccggataaag
     5821 aaaatcatcg tcgctataaa ctttgagcag cacagactgc aaacgtccgg tcttcaccgc
     5881 tgccgcagaa atttcccgcg ctttgtcgag ggtaaacaca gtgatgacgt cggttccctg
     5941 ttcaacggcg tcagaaacct gatgacaagg gatttgtacc agatgcccct gatgcgctac
     6001 cggcaaaccg gcgcggcgca tgactcgcgc ctctttgtaa tccaccgcca caatgccgct
     6061 gtagcccaga gccaacagtt tttctgccag ccacggatta cgaccgaatt gtttggtcat
     6121 cagatacagt tcaatgccgt aaagccgcgc cgtctcaatc agccgcttgc cgttttccag
     6181 tatctgatcc acgtcgatca cccagctgtc cggggcgatc ttgccctttt gccacaaact
     6241 tagtgcggcg gaaatcagcg ccgggttctg gcgtttcaat gcttctacaa acatcttgtt
     6301 gcctcgtttc tgttccttga attaaatatt catttttttg aatatttagg ttgtacgacg
     6361 tggatttatc gggagcttca aacctcttcg ttagcagcca tccataatcc ataaaggttg
     6421 gccagcaaat agccttcttc acacgggtta acttccagtg cgaattcctt caacaacacc
     6481 tgatgcaatt gcacaacggc ttgccagtgg ctggattgcg ccagctctgc cagcaattcg
     6541 ttatccagcg gttctatttc ttcaccacgg cgactgcgca tcagtgcact cgccatatgt
     6601 gtcatcgcca tcgttccttg ctcactgcgc accggtagat ggcactctgt ttccagtaca
     6661 ttgacgacct gcatcatgcc tttgcagatg tccttatcaa taacgcctgc atcgcaaagc
     6721 aggttgagtc tggtttccat atcagatgcc tttcgtttaa taactgggtt cctgttcccc
     6781 actgactact cgctgttgat gagcaagcag ggcatgttta tcaacaactg cacgcagcaa
     6841 ttgttgcatt gggtaatcat cgactcgcgt caaaaccacg gcttcggtgg cctgtaaaaa
     6901 tcgcggatcg tctgtgagcg gcgtcgcttc taatcccagc atggtcagtt cgttttccgc
     6961 cactacgttc cagataaccg cgtcaacatc gcctttaaca atgcgttgta aactctcgtg
     7021 ataagagaga tcgactcgtt ccacatcact atcgccaaaa aagacatcgg tcatgatttt
     7081 ctgatctgcc gaacggttat ccagccccac gcgcttcacg tttgcggact cgcctttacg
     7141 gcaaatcaac tggtgctcgc caacgtaggt atgcggtccc aactccagcg cgaggcataa
     7201 tcctttttgc gtgagataac tttccgccgc cagtcgcgaa accaccgcca tgtcatacac
     7261 gccgttaagt aaacactcca cgcgaatatc cgcgccgcgc atatgcgcat agtaaaaagg
     7321 aatgccatca aactgggctt tcaatccgct cgccaggcct tcgtacaaac gggtataggg
     7381 caagggcatc gcacatacca cgttgttgat atccacatga gtcagcaatg ctttgttatc
     7441 catctcgacc agataactgc cattgcgccc acggcgttca atccgtatcg ctccgcttga
     7501 ttccagcgtt ttcaacgcgg cctgcgtcag gccaaccgat gaacggcatt cagttgccag
     7561 ctcatctatg gttttcagtc gattaccgca cttttcaccc aacaaataac gggccagtgt
     7621 cgtgataacg acgccttctt ttttgataaa cgttcgacgc ataatatatt ttcaataaaa
     7681 tgaatgttta tatcttcagg aatttgaaga taaggcgcaa cagcgagatg tggaaacggc
     7741 gaggcgcttc acattttttc ggattattgc aaatgaggat aaaaaaaccg ggtttccccg
     7801 gtttttggca ttaatgttaa attacggctt cgccacaaaa ccaatcgctt cgtacaccgc
     7861 ttttagcgta cgggaagcgt gtgcgctggc tttttctgcg ccgtctttca tcacctgttg
     7921 caggaaggct tcatcgttgc ggaaacggtg gtaacgttcc tgcaattcag tcagcatacc
     7981 ggaaacggca tcagccactt cacctttcag atgaccatac atcttgcctt cgaactgttt
     8041 ttccagttcc gggatgctct ggcccgttac cgcagaaagg atatccagca ggttggatac
     8101 gcccgctttg ttctgcacat cgtagcgaac taccggcggc tcgtcggagt cagtgaccgc
     8161 gcgtttgatt ttcttcacta ccgatttcgg atcttccagc aggccgataa cgttattgcg
     8221 gttatcgtca gacttggaca ttttcttggt cggctccagc agcgacatta cgcgcgcgcc
     8281 agatttcgga ataaacggct ctggcacctt aaagatatcg ccgtacagcg cgttgaaacg
     8341 ctgggcgata tcgcggctca gttccaggtg ctgtttctgg tcttcaccca ccggcaccag
     8401 attagtttga tacagcagga tgtccgcagc catcagcacc ggatagtcaa acagaccagc
     8461 gttgatgttc tcggcataac gcgcagattt atctttaaac tgcgtcatgc ggctcagttc
     8521 gccgaagtag gtatagcagt tcagtgccca gcctaactgc gcatgttccg gcacgtggga
     8581 ctgaacaaaa atggtgcttt tctcaggatc gataccacaa gccagataca acgccagcgt
     8641 atccagcgtc gctttacgca gcttctgtgc atcctggcgc acggtgatcg cgtgttggtc
     8701 aacgatacag taaatgcaat ggtagtcatc ctgcatgttt acccactgac gcagcgcacc
     8761 catgtagtta ccaatggtca attcacctga gggctgtgcg ccactaaaaa cgatgggctt
     8821 agtcattttt cgattcctga ttttcgctat gcggaagccc taatgcgggc agaaggtcat
     8881 ttatagactg ataaattaca tcaggctggc tgagatcgat agcctcgccg tagttatatc
     8941 cgtaggttaa gccaactgat gggcaacctg ccgcttttgc tgcctgaata tcattgcgtg
     9001 agtcgccaac aaacaacatc tgttgtggag caattcccat ccgctcagcc accagtagca
     9061 gcgggtccgg gtgcggtttt ttattttgca catcatcacc accaatcacc acgctgaagt
     9121 atttggcgat atctaaggct tcgagcagcg gcgcgacgaa cggcgtcggt ttgttggtga
     9181 ccaagcctag cggcaggcct ttagcctgca acgcgcccaa cgtatcggca acgtgcggga
     9241 acaaaaacgt cccctcttcg gcaacctcgc catagtagcg atcgaacagt ttacgcagga
     9301 tacgtacctg ttcttctgcc ggaatgtcgt catcaacggg cggtttaccc attgttttac
     9361 gcagagtcgc acgttcctga cgcgcccagg tcaatgcgcg ctccatcaga atatctgcgc
     9421 cgttaccaat ccaggtaata acgcgttctt cacctgcgac aggcaactcc agcgcataca
     9481 gcgccatatc taccgcagca gcaagaccgg gtgcactgtc aaccagcgta ccgtcgagat
     9541 caaaagcgac gccgcgaata tcttcaaact tattcatgac ttacctttgc cagttcactg
     9601 cgcatttcat caatgacttt tttgtagtct ggctggtcga agattgccga accagcaaca
     9661 aacatatccg cgcccgccgc agcgatttcg ccaatgttgt tcaccttcac gccaccgtcc
     9721 acttccagtc gaatgtcaaa gccagactcg tcgatacgac gacgtacttc gcgcagttta
     9781 tccagtgttt gaggaatgaa agactgaccg ccgaaaccag gattgacgga catcagcagg
     9841 atcacatcca gcttatccat cacgtaatcc agatagctca gaggtgtcgc cgggttaaat
     9901 accagacccg ctttacagcc attttcttta atcagttgca gcgtgcggtc aacatgctcg
     9961 gaggcttctg gatgaaaggt aatgatgctg gcaccagcgg cagcgaaatc aggcacaatg
    10021 cgatcgacgg gtttcaccat cagatgtacg tcgatagggg cggtaatgcc gtagttacgc
    10081 aaggatttca gcaccattgg cccaatcgtc agattgggaa catagtggtt atccatgacg
    10141 tcaaaatgca cgacatcagc gccagctgcc agggcttttg cggtatcttc acccaggcgg
    10201 gcaaaatcag ccgacagaat tgagggggca atcaaatact gtttcatccg cttctccttg
    10261 agaattattt tttcgcgggt gaaacgactc ctggtttgta caaagccagc agttcgtcca
    10321 cctttttacg tgtgccgccg ttgctgctta tactgcgtcg aactttgacg acatgcaatt
    10381 ttgcgcgctg ataccactca cgcgttaaca tcgtatcgtg attggagatc agcactggaa
    10441 tatggcgatc aaccagacct tcagcgatct ccgccagatg cgcttgttgt tcaagcgtaa
    10501 aactgtttgt gtgatacgcc gtaaagttgg cagtcgcaga cagcggcgca taaggcggat
    10561 cgcaatagac gacagatgca tcatctgcgc gcgccatgct atcggcgtaa gactcacaat
    10621 agaaaaaggc attctgcgct ttttcagcga agtgatacaa ctctgcttcc gggaaatagg
    10681 gttttttgta gcggccaaac ggcacgttaa actcaccgcg cagattgtaa cgacacaggc
    10741 cgttgtaacc gtagcggttc aaatataaaa acagtaccgc ccgacggaac ggatcctggc
    10801 ttttgttgaa ctcttcgcgg aactgatagt aaacctcggc gcaatttgtt tcgggaacaa
    10861 acagctcgcg tgcggcctgt acgtactcat cagtacgcat cttcacaatg ttatagagac
    10921 tgatcaggtc gctattgata tcggcaagga tataacgaga aaagtcggtg ttgagaaaca
    10981 ccgacccggc acctacaaaa ggctcaacca gacattcgcc cttgggcaaa tgccgtttaa
    11041 tatcatcaag caggggatac ttgccccctg cccacttcaa aaaagcgcga tttttcttca
    11101 tgctgactaa ctaattacac cttctccggc tgtggagaaa gctccgacag catccagcgc
    11161 ttcaggcaac tcccgcaatg ataaaccatt gcgggagata accttgatta cttcagatcg
    11221 gcctgtacct gacgcagcgg tttcgcccac gggtttttgg cctggacatc tgctggcaat
    11281 gtagataccg cttttttcgc ctcttctttc gaagcataca cgccagaaac caggacatac
    11341 cacggctgac cattacgcgt cgtttcatag acaacgtagt ttttcagatt ctctttcttc
    11401 gcccaaccgt tcaggttgtc gtagttagag gaactgctca gctgcagagt gtaatggctg
    11461 gacggtgccg atttcaacga accaacatta cctgcggtct tccctccagc ggctggtgtt
    11521 gccgtggttt gcgccgggga tgccgtctgt actggagccg tagtcgccgt ctcttttggt
    11581 gctggcgtag aagtcgcagc cggtgccttt gtgctcgcca caggggcagc tggttcagtt
    11641 cgcttcggcg tctgtgcaac tggcttcggt tccgttttca cggttgcttg cggttttttc
    11701 ggttcaatca ccgcctgctg acgagccggg cgcgtagtgg ccggacgttc agcggtttgc
    11761 gttttcgccg tgtcacgcga tgcattgcca ttgcgaacag gcgcgacagt cgcgggttca
    11821 gtcggcaatg tagaattgac cgccacattg ttcaactgtt gctgattttg tggctgggtc
    11881 agcgcattgt tcaggtcacc ctgcacttca acacgttgtt gaccatccgt tgccgccggg
    11941 gtttgccctt gagtcggcgt agaagagatc ggcggcagag aaacatcctg ctgagtattt
    12001 tccgcagacg tggttcccgg cgctggctgc acgccattgg cctgatcggt cgcattgcct
    12061 gcaagatcaa tactcttctc gccagacgcg gtttgatcgc tggaagaggt cgagggggct
    12121 tttagcgcag aaccgatacc gatgatcaac agcagtagaa ccagaatgcc gacacccatc
    12181 atcatatact gacgagaagc gggtttactg gctgcttttt tgcgcttacg cggacgacgc
    12241 tctacgcgct cttcatccac ggtttcatct tcggattcgt caatttcttc ttcgatttcc
    12301 ggttcctcat tacgcgcttt ttgcgcacga gtcggacggc gatcgtcagt gtcatcaagt
    12361 tcaatatcat caaaattgat ctgcggttcg ccacgttcag tacgctcaga acgttcagaa
    12421 gattgacgag aacgaccagt acgacgatcg ctgggatcgg gtttcagctc gtcttctggt
    12481 ttgaattcat ccatttaaca ccccactaaa aggttaatgc ttaccacgtt gcaattaacc
    12541 tgaagctaat agaccgcttg ataagcggcc tgacctttct tgttgttacg ctgattgaca
    12601 atcggcaatg gcgttaagaa caagctcgtg cgaaacgccg ctgcgaactt cactcttacc
    12661 aattgccaac ggaagaatta agcgcatctc tcccgcaagg actttcttgt cacgcagcat
    12721 atgcggtaaa tacgcctgcg cggacatttc gcgcggccca ttgaccggta acccagcccg
    12781 cgtgagcagg gttataatac gctgcgtttt ggcagaacta aactgcccga gacgttccga
    12841 cgtccgcgcc gccatcacca tacccgcagc gaccgcttca ccatgtaacc aattgccata
    12901 ccccatttca gcttcaatgg catgaccaaa ggtgtgtccc agattcagta aagcacgtaa
    12961 cccggtttcg cgctcgtcgg cggcgacaac ttctgctttc agctcacaac aacggcgaat
    13021 acagtacgcc attgccggac cgtccagacg caacaacgca tccagattct cttccagcca
    13081 gttaaaaaac gcaccgtcaa gaataatgcc gtatttgatg acttctgcca gccccgacgc
    13141 taactcacgc gggggaagcg ttttcagaca gtcgagatcc accaccactg aagcgggctg
    13201 gtagaacgcg ccaatcatgt ttttaccgag gggatggttg accgcagttt tgccgccaac
    13261 ggaggaatcg acctgcgaca gtaacgtcgt cgggacttga atgaaacgta caccgcgctg
    13321 ataactcgcc gccgcgaagc cggtcagatc acccactacg ccgccgccaa gcgccaccag
    13381 agtagtatcg cgaccatgcg gcttttgtaa caatgccgta aagacggtat cgagtacagc
    13441 caggctttta tactgctcgc cgtcagggag gataacgcta tcgacgttaa cacccgcctg
    13501 ttcaagtacg ccgcggacct tatcgagata cagaggagcc agggtttcgt tggtgaccaa
    13561 catgacctgc tcgcccgatt tcagcggtaa gaatgaagct ggttcattaa acaaaccaga
    13621 tgcgatggta attgggtaac tacgttcccc gagagtaacg acaatcctct ccataacgcg
    13681 acatccacct taattactgt acccgcagac gagtgtatat aaagccagaa ttagttgctt
    13741 tccagcatgt gaataatctg gtttgcaacc actttagcgc tttgatcatc agtacgaatg
    13801 gtcacgtcgg caatctcttc atacagcgga ttgcgttcat tggccaacgc ttccagaact
    13861 tcacgcggcg gtgtttcaac gtgcagcaac gggcgttttt tatcacgctg cgtgcgtgca
    13921 agttgctttt cgatggtcgt ttcaagataa acgacaacgc cacgagcgga aagacggtta
    13981 cgcgtttcac gggatttcac agagccgccg ccagtagcca gcacaatacc ctgtttctcg
    14041 gtcaactcat tgatgacctt ttcttcgcga tcgcggaagc cttcttcgcc ttctaaatcg
    14101 aaaacccagc ccacatcagc tccggttcgt ttctcaatct cttgatcgga atcgtaaaat
    14161 tccatattga gttgttgagc taactggcgc ccaatagtgc tttttccggc acccataggc
    14221 ccaaccagaa agatattgcg tttctctgcc attttttcgg tactactaag actattcgtt
    14281 aatgataaac ccgcttcgct cagagagcgc cgcaggacat gaactgaaac ctcataagat
    14341 attgcgagag tcagactgaa aattatctca atactccagc gggtttggca actgaataaa
    14401 tcacccatcc ctctgcatat ctggtcgctg caagcgcgct gccttgctac caccgctctg
    14461 gcgataaatc accgggtaag attagcgtaa aaaagacagc aaaatgccgc ctgaatgata
    14521 aatcatcatc atggaacgca ggcagcgaag agcaccaact ctcaaatcgg tactccttgt
    14581 atgctaaata cctgcgcgcg tcaaatagat gaaacacgtt cagcgtaaaa acattaccgc
    14641 ttttacggct gtttactcac tggaaaccag tcgtggcgtg ataaacacca ctaactcgcg
    14701 tcgttcatct tcttttccgt catgacgaaa taattgcccg aaccagggaa tgtcgccaag
    14761 caacggtacg ctatcctgac ccgatttatt tttacgggta aaaatgccgc ccagcgccaa
    14821 cgtttctccg cttttgacct cgacctacgt ttcgatctcc tgcttatcaa tcgccagcac
    14881 ttcgccatct gcctgctgta gcacctgccc cggaacgttc tggctgatgt gtaatttcag
    14941 ccggatgcga cctttttgta acaccgtggg cgtaacttcc atccccagga cggcctcttt
    15001 aaattccacc gacgtcgcgc cactttcccc gctggaaacc tgatatggaa tttcgctccc
    15061 ctgtttaatg ctggcaggct gaagatgtga ggccagcaga cgcggactgg cgataatatc
    15121 caactgctgt ttttgttcga gcgcggaaag ctcaagatcc agtaaacgtc cgttgatgcg
    15181 tccaatgtta aaaccgatat gcgttgtcgc cgtcgctacg gagaggtcgc tgccaagcgt
    15241 agtgacttgc ccaacgccac caccttgttg cgcatcggcc aacgtccatt tcacacctaa
    15301 ctcacgcaaa cttttttcat taatggtgac aatatgcgcc gacagctcaa cctgcccgac
    15361 cggcagatcc atttgcgata cccactgttc aagcgtgctt aacgccgttt tgttatctcg
    15421 cagcaaaagg cgattggtgc gtttatcgac ggtcatactc cctttggcac tcagtagctt
    15481 ctcccccgct ttcgccagct ctccggcgtc ggcgtattgc agagtaatat tgcgattttc
    15541 cagcggcaga tttgcctgcg cccgcgtctg ctccgcctcc tggcgggcga tattgtcatt
    15601 ctgccaggca acggaatgca ccgagagaat gttgccctcc tggcgcgtta tcaatccggc
    15661 gcttttcact acagtttgta gtgcctgctt ccagggaacg tcagttaaat gtaacgacac
    15721 cgtaccgctg acgtctggcg aaaccaccag gttcaacttc tcctgttcag ccagcgcctg
    15781 caacacctga gctaccggaa cgtcatccac catcagcgtc actttttgcg gctttgccgc
    15841 ctgtacgccg ggtataagca tcaacagtag tgcggctatc cattgcttca tttgtatctc
    15901 cttgccgttg ccacaaccag tgtggcggtt cgcagtttgt cccggttccc agcgttagtg
    15961 cgtctggcgt cagctgtaag gtcgtccagc cgttttccag cacatcgttt tgctgcaccc
    16021 gtcgccattt tttttgccca tcttttatta caccgatgat gcgctcgcct cgccctacca
    16081 tcccctgata gtgccactgg ctaagttcgc taatccggca tagatcttcc ggcggtttaa
    16141 aagggtcacg cataccggtt aaaaggcaca atgcaatgcc tgctaacaac cagcgtttaa
    16201 ccctcatgcg gcgtctccag ttgtagcgtg aacaaaaggt catcaccttc cacgcttaac
    16261 aaaaaacggc tcacgctgac gttgcgctct gccagccgtg taaatgccga cggcactgct
    16321 tcccacagcg ttttcaacgc caactcacct ccctgcgcgg atggatgcca gtaaaccagt
    16381 tgcgcgccgg ataactgaaa atccagtggc gaaaagggca gcgttttttc ctcgctgaag
    16441 ggagtggtgt ctaccaggcg atacagtgca gcccgctgct ggtgatgatt tgcccgtagt
    16501 cgaattaatg cgtcgctctc ttcatggtgt gtcaatgaca gaaaaatgag cgtaactaac
    16561 accagcaacc agactgccca gcaaaactgg cggaggcggg gtgatgtggc gaaccaccag
    16621 tcaaagagca tgttcatcgc taaccttcct tgttaactga tactcaaatt gccagcgtcc
    16681 ctgcgcatcc tgctgcgtgg ccccccgctg attgagataa aaagaagcat cctggcggag
    16741 tgacgtttcc agcgcgttta acgcggcaat gcttgttgtc agccccttga tctccagcgt
    16801 tccctgctgc cagcttatcg ttgtcagcca ggcatgttcg ggtaaaagcg ccgccagtgc
    16861 ttccagcgca gattgccagt cgcgggtaaa ttgtcgttgg cgctgccgtt gcaaacgctg
    16921 ttcgcgtaac tgctggcgtt ccagcaaacg tggtttcgtt atctgtaagc tgcgggcgag
    16981 ttgttgttcc gcctgaagca aaacggcgtt tacgcgtgct tcggcattgc ttgtcagacg
    17041 cagtattagc gttatcccga cggccagcag cagaggcgca acgaacatca gcaaccagaa
    17101 acgaagaaaa gcagtccggc gttgctgtcg ccagggcaaa aaattaattg gcgggttcat
    17161 cagtacacct tcccaagcgc cagccccagc gcgatggcaa agtctccacc tggtggcggt
    17221 agcggcggct gacgaacaga aaccgcctcc caggggtcaa atccgccttc gccacatagc
    17281 gcgacgcttt cgggatcaac agataacgct gccgccagct ccttcgcgct agtcatcccc
    17341 accgccagtt tgcgccccca gctatagcgc gtcgcccaca gccactgttc gttatcacgc
    17401 caggccagac attgctgatg agaaggtaaa aaaggcagga atcgctgtaa tgcactggca
    17461 tccggagtga tcgcgctaac atgaacacgc aacctttctg ccagagtaag cagcgttgcc
    17521 agctctttgc tttgcgcggc agtcacgtta taggcgggac tgagtgagtc ttcgctgtaa
    17581 tcgaagcgca gggagtccgg atccatatcc agttcacggg ccatcgtccc ggacagccag
    17641 gccatttgct cccgctcacc gagggacatc gacgggcgtg gaaatgaccg ctgtaatgtg
    17701 cgactggcgg gaaacgccaa cataatgtga tgacgctgcg gcagttcacg actccacggt
    17761 aacaacgtgt tagccagccg ctgcacatca acaatccgcc catctttgat aatgtcgttc
    17821 tccagtggca accgccacca gcggtgcaat aagcattctt ttgcgctccg cacgatagca
    17881 accgctaccg cttcttgctg ttgtaaatgc aaaccaattt gccagttctt aaatgccatt
    17941 gtgatgatct ccttatcacc cgtcactctg acgggtatat caatgcgtct ggcttgcctt
    18001 tatactaccg cgcgtttgtt tataaactgc ccaaatgaaa ctaaatggga aatttccagt
    18061 gaagttcgta aagtattttt tgatccttgc agtctgttgc attctgctgg gagcaggctc
    18121 gatttatggc ctataccgct acatcgagcc acaactgccg gatgtggcga cattaaaaga
    18181 tgttcgcctg caaattccga tgcagattta cagcgccgat ggcgagctga ttgctcaata
    18241 cggtgagaaa cgtcgtattc cggttacgtt ggatcaaatc ccgccggaga tggttaaagc
    18301 ctttatcgcg acggaagaca gccgcttcta cgagcatcac ggcgttgacc cggtggggat
    18361 cttccgtgcg gcaagcgtgg cgctgttctc cggtcacgcg tcacaagggg cgagtaccat
    18421 tacccagcag ctggcgagaa acttcttcct cagtccagaa cgcacgctga tgcgcaagat
    18481 taaggaagtc ttcctcgcga ttcgcattga acagttgctg acgaaagacg agatcctcga
    18541 gctctatctg aacaagattt acctcggtta ccgcgcctat ggtgtcggtg ctgcggcaca
    18601 agtctatttc ggtaaaacgg tcgaccaact gacgttgaac gaaatggcgg tgatagccgg
    18661 gctgccgaaa gcgccttcca ccttcaaccc gctctactca atggatcgtg ccgtcgcgcg
    18721 gcgtaacgtc gtgctgtcgc ggatgctgga tgaaggatat atcacccaac aacagttcga
    18781 tcagacacgc actgaggcga ttaacgctaa ctatcacgcg ccggagattg ctttctctgc
    18841 gccatacctg agcgaaatgg tgcgccagga gatgtataac cgttatggcg aaagtgccta
    18901 tgaagacggt tatcgcattt acaccaccat cacccgcaaa gtgcagcagg ccgcacagca
    18961 ggcggtacgt aataacgtgc tggactacga catgcgccac ggctatcgcg gcccggcaaa
    19021 tgtgctgtgg aaagtgggcg aatcggcgtg ggacaacaac aaaattaccg atacgctgaa
    19081 ggcgctgccg acctatggtc cgctgctgcc tgccgcagtc accagcgcca atcctcagga
    19141 agcaacggcg atgctggcag acgggtcgac cgtcgtattg agtatggaag gtgttcgctg
    19201 ggcgcgtcct taccgttcgg atactcagca gggaccgacg ccacgtaaag tgaccgatgt
    19261 tctgcaaacg ggtcagcaaa tctgggttcg tcaagttggc gatgcatggt ggctggcgca
    19321 agtgccggaa gtgaactcgg cgctggtgtc gatcaatccg caaaacggtg ccgttatggc
    19381 gctggtcggt ggctttgatt tcaatcagag caagtttaac cgcgccaccc aggcactgcg
    19441 tcaggtgggt tccaacatca aaccgttcct ctacaccgcg gcgatggata aaggtctgac
    19501 gctggcaagt atgttgaacg atgtgccaat ttcccgctgg gatgcaggtg ccggttctga
    19561 ctggcagcca aagaactcac caccgcaata tgccggtcca attcgcttac gtcaggggct
    19621 gggtcagtcg aaaaacgtgg tgatggtacg cgcaatgcgg gcgatgggcg tcgactacgc
    19681 tgcagaatat ctgcaacgct tcggcttccc ggcacaaaac attgtccaca ccgaatcgct
    19741 ggcgctgggt tcagcttcct tcaccccaat gcaggtggcg cgcggctacg cggtcatggc
    19801 gaacggcggc ttcctggtgg acccgtggtt tatcagcaaa attgaaaacg atcagggcgg
    19861 cgtgattttc gaagcgaaac cgaaagtagc ctgcccggaa tgcgatattc cggtgattta
    19921 cggtgatacg cagaaatcga acgtgctgga aaataacgat gttgaagatg tcgctatctc
    19981 ccgcgagcag cagaatgttt ctgtaccaat gccgcagctg gagcaggcaa atcaggcgtt
    20041 agtggcgaag accggcgcgc aggagtacgc accgcacgtc atcaacactc cgctggcatt
    20101 cctgattaag agtgccttga acaccaatat ttttggtgaa ccaggctggc agggtactgg
    20161 ctggcgagca ggtcgtgatt tacaacggca tgatataggc ggaaaaacgg gtaccactaa
    20221 cagttcgaaa gatgcgtggt tctcgggtta cggtccgggc gttgtgacct cggtctggat
    20281 tggctttgat gatcaccgtc gtaatctcgg tcatacaacg gcttccggag cgattaaaga
    20341 tcagatctca ggttacgaag gcggtgccaa gagtgcccag cctgcatggg acgcttatat
    20401 gaaagccgtt cttgaaggtg tgccggagca gccgctgacg ccgccaccgg gtattgtgac
    20461 ggtgaatatc gatcgcagca ccgggcaatt agctaatggt ggcaacagcc gcgaagagta
    20521 tttcatcgaa ggtacgcagc cgacacaaca ggcggtgcac gaggtgggaa cgaccattat
    20581 cgataatggc gaggcacagg aattgttctg attaaaaagg cgcttcggcg cctttttcag
    20641 tttgctgaca aagtgcactt gtttatgccg gatgcgggtg aacgcgttat ccggccaaca
    20701 aaaaattgaa aattcaataa attgcaggaa cttcgtaagc ctgataagca ttatgcatca
    20761 ggcaaacttc acgcatttac actcgcccct gccctttcaa ccattcgcgc acgaggaaca
    20821 gcgcgctgac attacgcgct tcgttgaagt caggggcttc cagcaaatcc atcatatgcg
    20881 ccagcggcca gcgcacctgt ggtagcggct ctggctcatc gccttccagt gattccgggt
    20941 agagatcttg cgctaccacg atattcattt tgctggaaaa gtaagacggt gccatgctga
    21001 gcttcttcaa aaaagtcaga tcgttcgcac caaatccaac ctcttctttt agctcgcggt
    21061 tagcggcttc aaagacgctt tcacccggat caattaatcc tttcgaaaaa cctaattcgt
    21121 aggattcagt tcccactgcg tattcgcgga tcaggatcag gtgatcgtcc acaatcggca
    21181 caatcatcac tgcttcccgg ttggttggac gcattcgttc ataaacacgc cgcacgccat
    21241 tgctgaactc cagatccacg ctttcgacgg taaacagtcg ggaacgggct acagtttcaa
    21301 cattcagaat ggtgggtttt tgtaatgatt tgctcatcgt gggatctatg ctgtgaaatc
    21361 agcggttatt gtgcgatatc ggacacgctt tcggcaatgt gaattgcgcg ttatttacat
    21421 ttatgtaact taataaataa tcgtcctcaa atcaaattaa aagtcaatag gttgaaataa
    21481 ctccaggaat ttgctgatat tccgccttca gagggtttgc tatgatcagc ggttactgtg
    21541 atgtgcttaa tgatgctcaa gttaaactcc acgcttgccg atagccaacc gcagaatcat
    21601 gtattgtgtc cggtgcgact gaccacgcct gacagactaa gtaagatggg gaaagcatga
    21661 gcaccattgt gattttttta gctgctttgc tggcctgctc actacttgcg ggatggctga
    21721 taaaagtgcg atccagacga cgtcagctgc cctggaccaa cgccttcgcg gatgcgcaaa
    21781 cgcgtaaact cacatctgaa gaacgtagcg ccgttgaaaa ttatattgag agcctgacgc
    21841 aggtattaca ggtgcctggc ccaacgggag ccagcgcggc accgatctct ctggcgctga
    21901 atgccgaaag caacaacgtc atgatgctga cacacgctat cacgcgttat ggcatctcta
    21961 ccgacgatcc gaataaatgg cgttactacc tcgattcggt agaagtccac ctgccccctt
    22021 tctgggaaca gtacatcaac gatgagaata ccgttgaact gattcatacc gattcgctgc
    22081 cgctggttat ttcactcaac ggtcatacgc tgcaggagta catgcaggaa actcgcggct
    22141 atgccttgca acctgttccg tcaacgcagg cgtcgattcg cggggaagag agcgagcaaa
    22201 tagagctgct caatattcgc aaagaaacgc acgaagagta tgcgctgagt cgtccgcgcg
    22261 ggctgcgcga agcgttgctg attgtcgcct ccttcatgat gttctttttc tgcctgatta
    22321 ccccggatgt ctttgttccg tggctggcag gcggcgcgtt actgctgctg ggcgcaggtc
    22381 tgtgggggct attcgcgccc ccggcaaaat cctccctgcg ggaaattcat tgtctgcgcg
    22441 gtacaccccg tcgttgggga ttgtttggcg aaaacgatca ggaacagatc aacaatattt
    22501 cactcggtat tatcgacctg gtctatcccg cacactggca gccatacatt gctcaggatc
    22561 ttggtcaaca aaccgatatc gatatctatc tcgaccgcca cgtagtgcgt caggggcgat
    22621 atctttcgct gcatgatgaa gtaaaaaact ttccgttaca gcactggctg cgcagtacga
    22681 ttattgctgc gggctcgcta ttggtgctgt ttatgctgtt attctggatc ccgctggata
    22741 tgccgctgaa attcaccctc tcgtggatga aaggcgcaca gaccattgaa gccactagcg
    22801 taaaacaact ggctgacgct ggcgtgcggg ttggcgatac attgcgtatt agcggtacgg
    22861 gaatgtgtaa tattcgcact tccggtacct ggagcgcgaa aaccaattca ccttttttac
    22921 catttgactg ctcgcagatc atctggaacg acgcccgctc attgccatta ccagaatctg
    22981 aactggtcaa caaagcgacg gcattgaccg aagcagttaa tcgccagctg caccctaaac
    23041 cggaagatga atctcgcgtc agtgcctcat tacgttcagc aattcaaaaa tccggtatgg
    23101 tattgcttga tgattttggc gacattgttc tgaagacagc agatctgtgt tctgccaaag
    23161 atgactgtgt gcgactgaaa aatgcgctgg tcaatctcgg caacagtaaa gactgggacg
    23221 cgctggtaaa acgcgccaac gccgggaaac tcgatggcgt gaatgtgtta ttacgcccgg
    23281 tgagtgcgga atcgctggat aacctggtgg ccacctctac cgcgccgttt atcacgcatg
    23341 aaacggcgcg agcggcacaa tcactaaaca gtccggcccc cggcgggttc ctgattgtca
    23401 gcgacgaagg cagcgatttt gttgatcagc cctggccttc ggcatcactt tacgactatc
    23461 cgccgcaaga acagtggaac gctttccaga aactggcaca aatgctgatg catacgccgt
    23521 ttaacgccga aggtatcgtc acaaaaatct tcactgacgc caatggtacg cagcatattg
    23581 gccttcatcc gatcccggat cgttccggcc tgtggcgcta tctcagcacc acgctgctgc
    23641 tactgacgat gctgggtagc gccatttata atggcgtaca ggcctggcgt cgttaccagc
    23701 gccatcgtac tcgcatgatg aagattcagg cctattatga aagctgcctg aacccgcaac
    23761 tgatcacccc ttcagaaagc cttatcgaat aacacgtttg cgcggcaggt tatgctaccc
    23821 tgtcgcgcaa attgcttcac tctggagatt tccctcatgc atatcaacat tgcctggcag
    23881 gacgtagata ccgttctgct ggatatggac ggcacgttgc tcgacctcgc cttcgataac
    23941 tatttctggc aaaagctggt gcctgaaaca tggggcgcga aaaacggagt tacgccacag
    24001 gaagcgatgg aatatatgcg ccagcaatat cacgacgtac agcatacgct aaactggtac
    24061 tgtcttgatt actggagtga gcaactgggt ctggatatct gtgcgatgac caccgagatg
    24121 ggaccgcgtg ccgtactgcg tgaagatacc attccgtttc ttgaggcact gaaagccagc
    24181 ggtaaacagc gaattttgct caccaatgcg catccgcaca acctggcggt aaaacttgag
    24241 cataccggtc tggacgcaca ccttgattta ttactttcca cccacacatt tggttatccg
    24301 aaagaggatc agcggttatg gcatgcggtg gccgaagcta cgggtcttaa agctgaaaga
    24361 acgctgttta ttgatgacag cgaagcgatt ctcgatgctg ccgcgcaatt tggtattcgt
    24421 tactgcctcg gtgtgactaa tcctgactcc gggattgccg agaaacagta tctacgccat
    24481 ccgtcactga atgactaccg ccgcctgatc ccctcgctaa tgtgaaggag acgccatgaa
    24541 agagaaacct gctgttgagg ttcgactgga taaatggcta tgggctgccc gtttttataa
    24601 aacccgcgcg ctggcccgtg aaatgattga aggcggtaag gtgcattaca acgggcagcg
    24661 cagcaagcca agcaaaatcg tcgagctgaa tgccacgctc actctgcgcc agggaaatga
    24721 cgaacgcacg gtgattgtaa aggcgattac tgaacagcgt cgccccgcca gcgaggcagc
    24781 cttgctgtat gaagagactg cggaaagtgt agagaaacgc gaaaaaatgg cgctggcacg
    24841 taaacttaat gccttaacca tgccgcaccc ggaccgacgc ccggacaaaa aagagcgccg
    24901 cgacctgtta cgatttaaac acggcgacag tgaataactg tcacctgcaa gagagatgat
    24961 tatgccgcaa catgaccaat tacatcgcta tctgtttgaa aactttgccg ttcgcggcga
    25021 actggtaacc gtttcggaaa ccctgcaaca gatccttgag aatcacgatt atccgcagcc
    25081 cgttaaaaac gtgctggcag aactgctggt tgcgaccagc ctgttaaccg ctacgttgaa
    25141 gtttgatggt gatatcaccg tacagctgca aggcgacggt ccgatgaatc tggcggttat
    25201 taacggtaac aacaaccagc agatgcgcgg tgtggcgcgc gtgcagggcg aaattccaga
    25261 aaatgccgac ctgaaaacgc tggtcggcaa tggttacgtg gtgatcacca tcaccccgag
    25321 cgaaggcgaa cgctatcagg gcgtggttgg tctggaaggt gataccctgg cggcctgcct
    25381 ggaagattac tttatgcgtt ctgaacagct gccgacgcgc ctgtttattc gcaccggcga
    25441 cgtagacggc aaaccggctg caggcggaat gttgttgcag gtaatgcctg cgcaaaatgc
    25501 ccagcaggac gactttgacc acctggcaac gctaaccgaa accatcaaaa ccgaagaact
    25561 gctgacctta ccggcaaacg aagtgttgtg gcgtttgtat cacgaagaag aggtgacggt
    25621 ttacgatccg caggatgtgg agttcaaatg cacctgctcg cgtgaacgtt gcgccgatgc
    25681 gctgaaaacg ctgcctgatg aagaagtcga tagcatcctg gcggaagatg gcgaaattga
    25741 catgcattgt gattactgcg gtaaccacta tctgttcaat gcgatggata ttgccgagat
    25801 ccgcaacaac gcgtctccgg cagatccgca agttcattaa tgagtatgtc cggcagagag
    25861 ggctctgccg gattttacga cgtaatggcc tgatgcgctt atcagaccta cggattcaat
    25921 gcaatttatc gcaggtaaga taatgtgccg aatccaataa aaatcagata tgcgctgtca
    25981 ttcattcaga ttcaaatcca gtctttcgcc atccaccgta agatcgcgcc acactttagg
    26041 cttcgtcccc agttttccat cctccgcagc catgagtaat ttttctttcg ttattacagc
    26101 cgggagtgaa ttcatcgtaa tctctttcca ttctttccca gcaggatcaa taccatagac
    26161 aatgtatctt tcatcattaa aggcaaacag gatccgctct gccctgccat cactattcag
    26221 atcttgctct accagcacgc aggcatcttt ttcaatgcat atcattgcgt tatagcgatt
    26281 tttcatcact gccgaccaga atatcgtatc cggttttcca gaacctgagg caataaatac
    26341 attatctgct aatgcttttt ccgatatctg ctgctgaaga cgttgtactc catcaaaagc
    26401 catcagcaga ccccgcctgc gtttcgggtc tttcatatac tcggcatcgc ttttcagcga
    26461 ctcaagcgcc gcacgtccat agcgaccgct ctgttcgagc atatagagac ttacctggtc
    26521 tggcgtgttt ttgccgctct gataacgcgc catatgactg ttcacgctaa tgcgcatact
    26581 gtccagcacc ggagaattaa gaagcaccag tattaccaac accaataacg aaaccacaag
    26641 gttcactttg ccctgaagat caaggggatt ttgcccttta cgccagacga tactgacaaa
    26701 ataccccagc gaccatacca gtaacaccag caccgccagc acgccctgca agcggtcggc
    26761 agtccagccg tactgagcga cccgcagcca taacgcccag gcggcgacga acacatacag
    26821 cggagcaacc agcaaagcgg ttttaatcag gcaacgcaat ggccccgtcc agggaagtga
    26881 cgctttttgc ggatcgcgga caatagccat taagatcaat tgcaaaaagg ccagcgttaa
    26941 cagcaaccca gcggcggaga tgtggcgaga aatcgcgctc aggcccgtaa acggcagggt
    27001 gataataaac atcagggtta gcaatgatac taacggcagc aaccccgtgg cgattaacgt
    27061 gaacaacttt tgaatagagt cgattaaacg tgactgtgtt cgcgcgagga tcaccgccag
    27121 cgcggtaacc agacctaacg tgagataaat aaaccagtcg gttgcaaaaa agagcgtttt
    27181 gaaaaacgtg atgccaacaa gtttaaacaa ctcactccac agtaaaagca ccagccacgt
    27241 caggccattg gcgagaaaaa tcaccagtaa tataagtacg ttatgccata cagactggta
    27301 aaagtagcga tagcggctac tgtcattgcg gatgcgaagg ctttgttgta tccacggtag
    27361 caacaacatt gccatcagca gcagatagca gccaaaatcc caaagcgcct tttcagctct
    27421 ccagggggtc atgccatcag tttgccactt cagccagccg ctcattccca gcgtggcaat
    27481 aaacaccagc gccagccatt cccaaaggcg cttctgttta aaggagatca cggagaagag
    27541 taaaacggat gaaaacgcga cggtcacagg cacgccataa acaatccaac tgagattttt
    27601 tccggcaagc caggcgatca gcaggtagca aaccagcccc tgaagtaatc cggtagcaat
    27661 catcccccag cgtgttgccg gtgaaagttc aacgttatcc atgttggtta tccagaatca
    27721 aaaggtgggt taattatcgc ctccgggcag tagtattttg cttttttcag aaaataatca
    27781 aaaaaagtta gcgtggtgaa tcgatacttt accgattgaa tttgcatcaa tttcattcat
    27841 gaatgcgatt ccactcacaa tattcccgcc atataaacca agatttaacc ttttgagaac
    27901 attttccaca cctaaaatgc tatttctgcg ataatagcaa ccgtttcgtg acaggaatca
    27961 cggagttttt tgtcaaatat gaatttctcc agatacgtaa atctatgagc cttgtcgcgg
    28021 ttaacacccc caaaaagact ttactattcg ggcaatacat attggctaag gagcagtgaa
    28081 atgcgcgtta acaatggttt gaccccgcaa gaactcgagg cttatggtat cagtgacgta
    28141 catgatatcg tttacaaccc aagctacgat ctgctgtatc aggaagagct ggacccgagc
    28201 ctgaccggtt atgagcgcgg ggtgttaact aatctgggtg ccgttgccgt cgataccggg
    28261 atcttcaccg gtcgttcacc gaaagataag tatatcgttc gtgacgatac cactcgcgat
    28321 actttctggt gggcagacaa aggcaaaggt aagaacgaca acaaacctct ctctccggaa
    28381 acctggcagc atctgaaagg tctggttacc aggcagcttt ccggcaaacg tctgttcgtt
    28441 gtcgacgcct tctgtggtgc gaacccggat actcgtcttt ccgtccgttt catcaccgaa
    28501 gtggcctggc aggcgcattt tgtcaaaaac atgtttattc gcccgagcga tgaagagctg
    28561 gcaggtttca aaccagactt tatcgttatg aacggcgcga agtgcactaa cccgcagtgg
    28621 aaagaacagg gtctgaactc cgaaaacttc gtggcgttta acctgaccga gcgcatgcag
    28681 ctgattggcg gcacctggta cggcggcgaa atgaagaaag ggatgttctc gatgatgaac
    28741 tacctgctgc cgctgaaagg tatcgcttct atgcactgct ccgccaacgt tggcgagaaa
    28801 ggcgatgttg cggtgttctt cggcctttcc ggcaccggta aaaccaccct ttccaccgac
    28861 ccgaaacgcc gcctgattgg cgatgacgag cacggctggg atgatgacgg tgtgtttaac
    28921 ttcgaaggcg gctgctacgc gaaaactatc aagctgtcga aagaagcgga acctgaaatc
    28981 tacaacgcta tccgtcgcga tgcgctgctg gaaaacgtca ccgtgcatga agatggcact
    29041 atcgactttg atgatggttc aaaaaccgag aacacccgcg tttcttatcc gatctatcac
    29101 atcgataaca tcgttaagcc ggtttccaaa gcgggccacg caactaaagt tatcttcctg
    29161 accgcagatg ctttcggcgt attgccgccg gtttctcgtc tgactgccga tcaaacccag
    29221 taccacttcc tctctggctt caccgccaaa ctggctggta cagagcgcgg catcaccgaa
    29281 ccgacgccaa ccttctccgc ttgcttcggc gcagcgttcc tgtcgctgca cccgacgcag
    29341 tacgcagaag tgctggtaaa acgtatgcag gcggcaggtg cgcaggcgta tctggttaac
    29401 accggctgga acggcactgg caaacgtatc tcgattaaag atacccgcgc tattatcgac
    29461 gccatcctca acggttcgct ggataacgca gaaaccttca ctctgccgat gtttaacctg
    29521 gcgatcccaa ccgaactgcc gggcgtagac acgaagatcc tcgatccgcg taacacctac
    29581 gcttctccgg aacagtggca ggaaaaagcc gaaacgctgg cgaaactgtt tatcgacaac
    29641 ttcgataaat acaccgacac ccctgcgggt gccgcgctgg tagcggctgg tccgaagctg
    29701 taatgatttg aagctggaga atatctatcc agtatcttat agaaagcaaa acgggaggca
    29761 ccttcacctc ccgttttttt acccttcttt tgtcatgccc tgcgcccgcg ttaccggcac
    29821 tggcagccag gcgcgaatgg aaagcccgcc ccgttcgctg gtgccaagct ccagcatccc
    29881 gttatggtta tccacgatac gctgcacaat cgccagccct aatcccgtgc cgctaatagt
    29941 gcgcgcactg tcgccacgaa caaacggctg gaacaggtgc ttacgctgtt ctggcgcaat
    30001 acccggaccg tcatcttcta cctgaaacca ggcgcgattc ggctccgtgc cgctgctgac
    30061 tttgatccag ccattgccat agcgggcggc gttcaccacc atattcgcta ccgcgcgttt
    30121 gatcgacagc gggtgcattt tcacctcaat gctaccgggg taaagcgcag tttcaatttc
    30181 ccgctcatag ccactttcgg cagcaataac ctcaccgagt actgcgttga gatccgccat
    30241 ttccatcggc atctcctgcc cggtgcgcag gtagtcgata aactgctcaa tgatggcgtt
    30301 gcactcttcg atatctttat tgatcgattc tgccaggtag ccatcctgct cgctcatcat
    30361 ctcggtcgcc agacgaatac gcgtcagcgg cgtgcgcaga tcatggctta cccccgccat
    30421 cagcagcgta cggtcatccg ccaattgctt aacaccagcc gccatatggt taaaggcgcg
    30481 ggtaacggaa cgcacctccg aagcgccata ttcacgcagc ggcggcggaa taatcccttt
    30541 acccacctgc aaggctgcgt gttcgagatc gaccaacggt cggttctgga tacgaataaa
    30601 cagccacgcc ccgcctatcg ccaatagcat aatcgccaac gtataacgga acagcgggga
    30661 gaaatcgccc tgatgaattt cggttagcgg cacgcgtacc cagatattgg gcgacaacca
    30721 ggttttcagc cagacgacag gcgaactttt gttaacctcg acgcgaactt ccgtcggacc
    30781 gcccagttgc tgcgccatct gatggcttaa gaattcatag tgttgcgccc aacgcagacc
    30841 tgcctcttcg gcagcttcgt tggaatagag agagatcccc agctcacggt agatctcccg
    30901 acggaaagcg ggaggcacaa ccaactgcgt gccgtcctcc agttgcagtt tgtcggtcat
    30961 caacatacgc acttcgtacg cgaggacttt attaaactgc tggaggctcg gcaaaatcgc
    31021 gaagttcagc accaccagat aagtcgtcac caggctgaca aacagcaagg tgacgatgag
    31081 caataacgta cgggcaaatg aacttcgtgg cgagaagcgc aatcgcctca tgctttagaa
    31141 ccgtccggta caaagacgta gcccagaccc cagacggtct gaatgtaacg cggatgcgcc
    31201 ggatcttctt ccaccatgcg gcgcagacgc gaaatctgca cgtcgatgga gcgttccatt
    31261 gcggaatatt cacgaccacg ggcaaggttc atcagcttat cgcgggagag cggctcacgc
    31321 ggatggctga ccagtgcctt cagtaccgca aactcaccac tggtgagcgg catcggctcg
    31381 tcttcgcgga acatttcgcg cgtaccgagg ttaagtttga acttaccgaa agcaattacc
    31441 gcctcttcct gtgacggtgc gcctggcagt tcgttcgcct gacgacgcag caccgcacga
    31501 atccgggcca gcagttcacg cgggttaaac ggttttggaa tgtaatcgtc agcgccaatc
    31561 tccaggccaa cgatacggtc cacttcttcc cctttcgccg tcaccataat gatcggcatc
    31621 ggattgctct ggctacgaag acgtcggcaa atcgacaagc catcttcacc aggtaacatc
    31681 aaatccagta ccataagatg gaaagattca cgagtcagca ggcgatccat ctgttctgca
    31741 ttagcgacgc ttcgaacctg gaagccttgt tcggtgagat aacgttccag cagcgcgcgc
    31801 aggcgcatgt cgtcatcaac caccagaatc ttgtagttct cttgcattgt ttgtactccc
    31861 aaaggttcgc aacaatttgt aagcgtgtat tcttaaaaaa gctcacgttc gtcaccagct
    31921 aaatctggta tgaatttcag cctaaattgt tacaaagcat attaaacagc agcttaagta
    31981 tacaatttat tcggcgaaac attattgatt ctgttgatat gatcacgtta tacccaatgt
    32041 gcgcattatc aaacagacaa agggaatcaa cgagatgaaa acgcccctgg ttacccggga
    32101 agggtatgaa aaactcaaac aagagcttaa ttatctctgg cgtgaagaac gcccggaggt
    32161 cacaaagaaa gtgacctggg ccgcaagtct gggcgaccgc agcgaaaatg ctgactatca
    32221 gtataataaa aagcgcctgc gtgaaatcga ccgtcgcgtg cgctatctca ctaaatgcat
    32281 ggaaaatctc aaaatcgtcg attactcccc gcagcaggaa ggcaaagtct tttttggtgc
    32341 gtgggtggag attgaaaacg acgatggtgt gactcaccgt ttccgtattg tcggctacga
    32401 tgaaattttt ggccgtaaag attacatctc tatcgattcc ccgatggccc gcgcattgct
    32461 gaaaaaagaa gtcggcgatc tggcggtggt gaatacccct gccggggaag cgagctggta
    32521 tgttaatgct atcgagtacg tgaaaccgta aggaagagtc ttaacctcct gccgatggct
    32581 ggcatttttg ccagccagtc cgtataacta tcccctgatt tttgatccga aaagatgaac
    32641 tcaaaccatg atgaatgatt cgttctgccg cattattgcg ggtgaaattc aggcgcgccc
    32701 ggaacaggtt gacgctgccg ttcgcctgct tgacgaaggg aataccgtgc cgtttatcgc
    32761 acgttatcgt aaggaaatca ccggcggtct ggatgacacg cagctgcgta atctggaaac
    32821 gcgtctgagc tatctgcgcg agctggaaga gagacgtcag gcgatcctca agtctatttc
    32881 cgagcaaggc aaactcaccg atgatctggc gaatgccatc aacgccaccc taagcaaaac
    32941 cgaactcgaa gacctctacc tgccctacaa acctaaacgc cgcacccgtg ggcaaatcgc
    33001 cattgaagca gggcttgagc cgttggccga tctgctgtgg agcgatccgt cacacacgcc
    33061 agaagtcgcc gctgcacaat atattgatgc cgataaaggc gtggcagata ccaaagccgc
    33121 gctggacggc gcgcgctata tcctgatgga acggtttgcc gaagatgccg cgctgctggc
    33181 gaaagtgcgt gattatctgt ggaagaacgc gcatttggtc tcaaccgtgg tgagcggtaa
    33241 agaagaggaa ggggcaaaat tccgcgacta tttcgatcat cacgaaccac tgtcaacggt
    33301 gccttctcac cgcgcgctgg cgatgttccg tggtcgtaac gaaggcatac tccagctttc
    33361 gctgaatgcc gatccgcagt tcgatgagcc gccgaaagag agctattgcg agcaaatcat
    33421 catggatcac cttggcctgc gcctgaacaa tgccccggcg gatagctggc gcaaaggtgt
    33481 ggtgagctgg acgtggcgca tcaaggtgtt gatgcatctg gaaactgaac tgatgggcac
    33541 cgtgcgcgaa cgcgcggaag atgaagcaat caacgtcttt gcccgtaacc tgcacgatct
    33601 gctgatggcg gcccctgccg ggctgcgtgc aacgatgggc ctcgatccgg gtctgcgtac
    33661 cggggtaaaa gtggcggtag tcgatgcaac tggcaaactg gtggcgaccg ataccattta
    33721 tccgcatacc gggcaggccg caaaagcagc gatgaccgtt gctgcgctgt gtgaaaagca
    33781 taacgttgaa ctggtggcga tcggcaacgg tacagcttct cgcgaaaccg aacgtttcta
    33841 tctcgacgtg cagaagcagt tcccgaaagt gaccgcgcag aaagtgatcg tcagcgaagc
    33901 tggcgcgtcg gtttactcgg cttccgagct ggcagcacag gaattcccgg atctcgacgt
    33961 ttcgctgcgt ggcgcggtgt ctatcgcccg tcgtttgcag gatccgctgg cggagctggt
    34021 gaaaatcgat ccgaaatcta tcggcgtagg tcagtatcag catgacgtca gccagacgca
    34081 gctggcacgc aaactggatg cagtagtaga agactgcgta aacgccgttg gcgtcgatct
    34141 caacaccgct tctgttcctc tattaacccg cgtggcgggc ctgacgcgca tgatggcgca
    34201 aaacatcgtg gcctggcgcg atgagaacgg ccagttccag aaccgtcagc aactgttaaa
    34261 agtgagccgt ctggggccga aagccttcga gcagtgcgcg ggcttcttgc gcattaacca
    34321 cggtgataac ccgctggacg cttctaccgt tcacccggaa gcctatccgg tggtggaacg
    34381 cattctggca gcaacacagc aggcactgaa agatctgatg ggtaacagca gcgaactgcg
    34441 taacctgaaa gcgtctgact ttactgatga gaaattcggt gtgccgacgg taaccgacat
    34501 catcaaagag ctggaaaaac cgggtcgcga tccgcgtccg gaatttaaaa ccgctcagtt
    34561 tgccgatggc gtcgagacaa tgaacgacct gcaaccgggt atgatcctcg aaggtgcggt
    34621 gaccaacgtc accaactttg gcgcgtttgt cgatattggc gtgcatcagg acggcctggt
    34681 tcacatctct tcattgtcga acaagtttgt ggaagatccg cataccgtgg tgaaagcggg
    34741 cgacattgtg aaggtgaaag tgctggaagt ggatcttcag cgtaaacgta tcgccctgac
    34801 catgcgtctg gacgagcagc ctggcgaaac caacgcccgt cgcggcggcg gtaatgaacg
    34861 cctgcaaaac aaccgcccgg cagccaaacc gcgtgggcgt gaagcgcagc ctgccggtaa
    34921 tagcgcgatg atggatgcgc tggcggcggc aatgggcaaa aaacgttaaa cgcccggact
    34981 ggcccctaaa cctgcgcgat taggcgttca cgccacatct ggcaacgaac gccttgcctg
    35041 acataaaagt gccggagaat atctccggtc tttttattcc acaagcaaac tcataataga
    35101 ttccgttaat atttatcact tcattaacaa ctgaaacctt aattaaacat taaccagccc
    35161 taataattcg tcatgcgaat tatattttcg ctgcgatata accttgagcc atatcaacat
    35221 tgagtcagat tattattcaa accaacattc acacacattt taagtattgc tgatagaaac
    35281 cattctcatt atcattgtac tgttgattat ttaatctctc cttcgttggc aaatcatctg
    35341 gtctcatgtc gctgtcaaac gccccatgag gtagttatcc agttaatgag aaacaagtag
    35401 gcacctatgc aatacactcc agatactgcg tggaaaatta ctggcttttc ccgtgaaatc
    35461 agcccggcat atcgccaaaa actgctttct cttggcatgt tacctggctc ctcttttaat
    35521 gtggtgcacg tcgctccact cggcgacccc attcatatcg aaacccgtcg tgtgagcctg
    35581 gtattacgca aaaaagatct ggccttatta gaagtggaag cggtttcctg ttaatacggt
    35641 gataacaaca atgaaaaaat taaccattgg cttaattggt aatccaaatt ctggcaagac
    35701 aacgttattt aaccagctca ctggctcacg tcagcgtgta ggtaactggg ctggcgttac
    35761 cgtcgaacgt aaagaagggc aattctccac caccgatcat caggtcacgc tggtggacct
    35821 gcccggcacc tattctctga ccaccatctc atcgcagacc tcgctcgatg agcaaatcgc
    35881 ctgtcactac attttgagtg gcgacgcaga cctgctgatt aacgtggtgg atgcgtctaa
    35941 ccttgagcgt aacctgtacc tgacgctgca actgttggaa ctcggcattc catgcatcgt
    36001 ggcgctgaac atgctcgaca ttgccgagaa gcaaaatatt cgcattgaaa ttgatgcgct
    36061 gtcggcgcgt cttggctgcc cggtgatccc gctggtttca acccgtggtc gcggtattga
    36121 agcgctcaag ctggcgattg atcgctataa agctaacgag aatgtggaac tggtgcatta
    36181 cgctcagccg ctgctcaacg aagcagattc actggcaaaa gtgatgcctt ccgacatccc
    36241 gctgaaacaa cgtcgctggc tgggcctgca aatgctggaa ggcgatatct acagccgcgc
    36301 ctacgccggt gaagcgtcgc agcatctgga tgccgccctc gcccgtctgc gtaatgagat
    36361 ggacgatccg gcgctgcaca ttgccgatgc gcgttaccag tgcatttctg ccatctgtga
    36421 tgtggtaagc aacaccctga cggcagaacc cagccgtttc accactgcgg tagataaaat
    36481 cgtgctcaac cgtttcctcg gtctgccgat tttcctcttt gtgatgtacc tgatgttctt
    36541 gctggctatc aacatcggcg gggcgttaca gccgctgttt gacgtcggct ccgtagcact
    36601 ctttgtgcat ggtattcagt ggattggcta cacgctccac ttcccggact ggctgactat
    36661 cttcctcgcc caaggcctgg gcggtggtat taacaccgtg ctgccactgg tgccgcagat
    36721 cggcatgatg tacctgttcc tctccttcct tgaggactcc ggctatatgg cgcgtgcggc
    36781 gtttgtgatg gaccgtctga tgcaggcgct gggcttgccg gggaaatcct ttgtaccgct
    36841 gatcgttggt ttcggttgta acgtaccgtc ggtaatgggc gcacgtacgc ttgatgcacc
    36901 gcgtgaacgt ctgatgacca tcatgatggc accgtttatg tcctgcggcg cgcgtctggc
    36961 tatcttcgca gtattcgcgg ctgccttctt cgggcagaac ggtgcgctgg cggtcttctc
    37021 gctgtatatg ctgggtatcg taatggcggt gctgactggc ctgatgctca agtacaccat
    37081 catgcgcggt gaagcgacgc cgtttgtcat ggagctgccg gtctaccatg taccacacgt
    37141 taaaagcctg attatccaga cctggcagcg tctgaaaggc ttcgttctgc gtgctggtaa
    37201 agtgattatc atcgtcagca ttttcctgag cgccttcaac agcttctcgc tgagcgggaa
    37261 aatcgtcgat aacatcaacg actcggcgct ggcttccgtc agccgggtga tcaccccggt
    37321 cttcaagccg attggcgtac atgaagataa ctggcaggca acggttggcc tgtttacagg
    37381 tgccatggcg aaagaagtgg tggtgggtac gctcaacacc ctctacaccg cagaaaatat
    37441 tcaggacgaa gagttcaatc cggcggaatt taacctcggt gaagagctgt tcagtgcggt
    37501 agatgaaacc tggcagagcc tgaaagacac cttcagcctt agcgtactga tgaaccccat
    37561 tgaagccagc aaaggcgacg gcgaaatggg taccggggcg atgggcgtga tggatcagaa
    37621 attcggtagc gcagctgccg catacagcta cctgattttc gtcctgctgt acgtaccgtg
    37681 catctcggtg atgggtgcta tcgcccgtga atcaagccgt ggctggatgg gcttctccat
    37741 cctgtggggg ctgaatatcg cttactcgct ggcaacattg ttctatcaag tcgctagcta
    37801 cagccagcat ccaacttaca gcctggtgtg cattctggca gttatcctgt ttaacatcgt
    37861 ggttatcggt ctgctgcgcc gcgcgcgtag ccgggtagat gtcgaactgc tggcaacgcg
    37921 caaatcggta agcagttgct gtgcggccag taccactggc gattgccact aaggacacga
    37981 caatggcttc gctcattcaa gtgcgtgatt tactggcatt acggggccgt atggaagagg
    38041 cccagattag ccagacactg aacactccgc agccaatgat taacgccatg ctgaaacagc
    38101 tagaaagtat gggaaaagcc gtgcggattc aggaagaacc tgacggctgt ctttccggca
    38161 gttgtaaaag ctgcccggaa ggaaaagcct gtctgcacga atggtggacg ttgcgttaac
    38221 cttgtttcac tgcctgtttt gaaagcccgg tatgcgtttg catccgggct tttttgcgtg
    38281 cggctttcca taaaaatgca acccttgcag cgcgacgtaa gttcctctga aagcgtctcg
    38341 cagggatgaa aactcgctaa tgcacaggtg tggagtggtg cgtagagtcg cggcattcaa
    38401 acaacaggtg aaggaacgcc atgagcaaaa agcagagttc caccccacac gatgcgctgt
    38461 tcaaactctt tttacgccaa ccggagacgg cgcgcgattt tcttgcgttt catttaccgg
    38521 cacctattca cgcgctctgt gatatgaaaa ccctcaagct ggagtcgagc agctttattg
    38581 atgacgatct gcgtgaaagc tattccgacg tgctgtggtc ggtgaaaacg gaacaagggc
    38641 caggatatat ctattgtctg attgaacatc aaagcacctc aaacaaactg atcgcatttc
    38701 gcatgatgcg ttacgccatt gccgcaatgc aaaatcacct ggatgccgga tacaaaacat
    38761 tgccgatggt ggtgccattg ttgttttacc acggtattga aagcccctat ccctattcgc
    38821 tgtgttggct tgattgtttc gccgatccca aactggcaag gcagctttat gcctccgcat
    38881 ttccgctgat tgatgtcacc gtcatgcctg atgatgaaat catgcagcac cgacgtatgg
    38941 cactgctgga gctaattcaa aaacatatac gtcaacgcga cctgatgggg ctggtagagc
    39001 aaatggcctg cttattaagt agtggatacg ctaatgacag acaaatcaaa gggctgttta
    39061 attacatacg acaaaccggc gatgcggtac gttttaacga ttttatcgac ggcgttgccg
    39121 aacgttcacc gaaacacaag gagagtttaa tgactattgc ggaaagattg cggcaagaag
    39181 ggtttcagaa aggtatacga gctatcgcca aaaccatgct ggatgccgga gtttctcttg
    39241 aggacgtttt gcgatttacc agcctgacgg ttgaagacct tgctgaaata aatcatcaat
    39301 aacgtaaagg atggttttaa tgacagatgc agaaagagaa tatcttatta tttacctgat
    39361 tggttattta ttgggtcgac gtctggaagc cctggatatc gccaaaagaa tgctgtaatc
    39421 cggagtccct cttgcggaca tcatgcgttt taccggggtg tcagaagaag agttggctgc
    39481 ggcgagtcag taaaattctg tctcgccatt tcgaaagcca cctacaccct ctgcttcaac
    39541 gccaccagca gatgacaaaa ctcgtccgga tgtgaaataa acggcgcatg ggccgctttg
    39601 gcgaagatat atgattcgct gtgaggccaa agtttatcca gcatcggcac cactttgcgc
    39661 ggcaccagac cgtcgagata gccatacaat cgcaaaaacg gcatgggcac gttttgcagc
    39721 ggctgacgga gatcgaccgt tttcaggatt tccagcccgc cattaagcac gtcaacctcc
    39781 ggcatcggta acgccagaac agttttcttc aacgcccgcg catcctggcg cgccgtttca
    39841 gtacccatgg tttgtaacgc caggaaccgc tccactgtac gctgaaaatc atcactgagt
    39901 tgctgctgaa atcccgccag cacgtccggt tttatccccg gccactcgtc gcgagcacta
    39961 aaacaaggtg acgacgccac ggtgaccagc gcctgaaccc gctcgggatg ggttaacgca
    40021 atctggcttg ccaccagccc gcccagactc cagcctaacc aaatggcttt atctggtgcc
    40081 tgtcgcagca cgacttcggc catatcagca agtgatagcg caccaaatcc ccggctacgc
    40141 ccgaagccgg gcaggtcaac aaggtgcagc gtaaaatgcg agctaagttc ctcgtcaatg
    40201 caacgccaca cttcggcatt cagtccccat ccgtgcagca gcacaagatg aacattcccc
    40261 tgacctttgg tctgccacca gatgttattc atccgctatt gttctctttt tacttacaag
    40321 gatgaacata tgctaacagt accgggatta tgctggctat gccgaatgcc actggcgtta
    40381 ggtcattggg ggatttgttc ggtctgctct cgcgccgccc gtacagataa aacgttatgc
    40441 ccacaatgtg gattacccgc cacacactct catcttccct gcggtcgctg cctgcaaaaa
    40501 ccgctgccct ggcaaagact ggtcacggtc gccgactatg cgccgccatt aagtccgctc
    40561 attcaccagc ttaagttttc ccggcgcagt gaaatcgcca gcgccctgtc acgtctgtta
    40621 ctattggaag tcttacacgc tcgtcgcacc accggcttgc aactgcctga tcgcatcatc
    40681 agcgttccgt tatggcagcg ccgtcactgg cgtcggggat ttaatcagag cgatttgctg
    40741 tgtcagccgt tatcacgctg gctacactgc caatgggata gcgaagccgt cacacgaaca
    40801 cgggccaccg cgacccagca ttttcttaac gcccggctac gcaaacacaa cctgaaaaat
    40861 gcctttcgcc ttgaattgcc tgtgcaaggt cgccatatgg tgattgtgga tgatgtcgtt
    40921 accaccggaa gtaccgtcgc agagattgcg cagttgcttt tacgcaatgg tgcggcgact
    40981 gtccaggtct ggtgcctttg tcgaaccttg tagagcctcg atgatgggcg tattataacc
    41041 aactaaaata gtcaactatt aggccattac tatgatccgt atttccgatg ctgcacaagc
    41101 gcactttgcc aaactgctgg caaaccagga agaagggaca caaatccgcg tatttgtgat
    41161 taaccctggc acgcctaacg ctgaatgtgg cgtttcttat tgtccgccgg acgctgtgga
    41221 agccaccgac actgccctga aatttgacct gctgaccgcg tatgttgatg agttaagcgc
    41281 tccttacctg gaagatgcag agatcgattt tgttaccgac cagttgggtt cgcagctgac
    41341 gctgaaagcg cctaacgcca aaatgcgtaa agtggctgac gatgcgccgc tgatggagcg
    41401 cgtggagtat atgctgcaat cacagatcaa cccacagctt gctggtcacg gtggtcgcgt
    41461 ttcgctgatg gaaatcaccg aagacggtta cgctattttg caatttggcg gtggctgtaa
    41521 cggttgttcc atggtcgacg tcaccctgaa agaagggatc gaaaagcagt tgctgaacga
    41581 attcccggag ctgaaaggtg tacgcgatct caccgaacac caacgcggcg agcactctta
    41641 ctactaagtt ctctcccctt ttgtcgattg cccgatgtga cgctaaagcg tcttatcggg
    41701 cctgtacgtt gcacgagatt ctgacgttag cgcagaatta tctcagcaaa tccaatctct
    41761 tcgtttcccc acgataatat gaccaacctc tcataattta aatttacccc gctctggtga
    41821 ttctcaagca cgtaatgtta cccgtatcat tctcatgggc accaaacaca ctcataacat
    41881 ctgactacac taactcgttt taggggtcat cagccgtggt gccgtaacat ctctgttccc
    41941 agttgggaca accaggattt tccgtcagaa acctgacgtt acccataaca aattaaaggc
    42001 caggtaaatc atgccattag tcattgttgc tatcggtgta atcttgttat tactcctgat
    42061 gatccgcttc aaaatgaacg gcttcatcgc tctcgtcctc gtggcgcttg ctgttggatt
    42121 aatgcaaggg atgccgctgg ataaagttat tggctccatc aaagccggtg tcggcggaac
    42181 gctcggtagc cttgccctga tcatgggttt tggtgcgatg ctgggcaaaa tgctggcaga
    42241 ctgcggtggt gcacaacgta tcgctaccac gctgattgcc aaatttggta aaaaacacat
    42301 ccagtgggcg gtggtactga ccggttttac cgttggtttt gccctgttct atgaagtagg
    42361 tttcgtgctg atgctgccgc tggtgtttac catcgcggct tccgcgaata ttccactgct
    42421 gtatgttggt gtaccaatgg cggctgcact gtctgtgacc cacggcttcc tgccaccgca
    42481 tccgggtccg actgcgattg ccaccatttt caatgccgat atgggtaaaa ctctgctgta
    42541 cgggactatt ctggcaatcc cgaccgtgat tctcgctggt ccggtttacg ctcgcgtgtt
    42601 gaaaggtatc gataagccga tcccggaagg tctgtacagc gcgaaaacct tcagcgaaga
    42661 agagatgccg agctttggcg tcagcgtctg gacctctctg gtgccggtgg ttctgatggc
    42721 gatgcgtgcg attgccgaaa tgatcctgcc gaaaggtcac gctttcctgc cggtagcgga
    42781 gttcctcggt gacccggtaa tggctacgct gattgccgtg ctgatcgcga tgttcacctt
    42841 tggtctgaac cgtggccgtt caatggatca gattaacgac acgctggttt cttccatcaa
    42901 aatcattgcg atgatgctgt tgattatcgg tggtggcggt gcgttcaagc aggtgctggt
    42961 agacagcggc gtggacaaat acattgcttc catgatgcac gaaaccaaca tttctccgct
    43021 gctgatggcc tggtcgattg ctgccgtact gcgtatcgcg ctgggttccg caaccgtcgc
    43081 ggcaatcact gcgggtggta tcgcggcacc gctgattgca acgacgggtg ttagcccgga
    43141 gctgatggtt attgcggttg gttccggtag tgtgattttc tctcacgtga acgatccggg
    43201 cttctggctg ttcaaagagt actttaacct gactatcggc gagaccatca aatcctggtc
    43261 gatgctggaa accattatct cggtgtgcgg tctggtaggc tgtctgctgc tgaatatggt
    43321 gatttgataa tactgatggc cggatgcggc gtgaacgcct tatccggcct acgattcggc
    43381 attaattagt aggccggtaa gacgcgtcaa gcgtcgcatc cggcatcaac cgcactctac
    43441 ttcttcttcg ctgccgctct gcgccgtctg tccaaatcct tcagcaactt gttcacacca
    43501 tcatcggcaa acatcgactc aagcgttgcg gaaagtttac gtcgccagtt tttatactgg
    43561 taactggtgc cgggaatatt taccggatct gccatatcca gccagtcttc cggctggagc
    43621 cctaacaggg cgctgttgct atcggcgatg tagcgctgta agccacgatt cagcgtcggt
    43681 gtcatcgaca tcaacgatgc cttatgcccg gcacgttttg gcaggcaacc atatttatgc
    43741 agagcatcca gcagcccttg cttcgccagt tcgcgatcct gatacagacc gcgcagtacc
    43801 acttcatccg gatacagccc cagcgttttg cccagcgtta aatccccact ctcccagtag
    43861 ccgcgtagcg taggcaggtc gtgtgtcgcc gcaaccgcca tcgactgctc tgggtacgct
    43921 ttcggtgcgc ggaatgtctt ctcgtggtcg ttttcgaaat agagcacttt gtaggagtac
    43981 acaccgctgc tgcgcagctt accgacaatc tctaccggta cggtaccgag atcttcacca
    44041 atcaccatac agcgatgacg tttactttcg agtgccagaa tcgagagcag atcatccacc
    44101 ggatagtgaa catacgcgcc ctgatctgcc gtctcgccat acggtatcca ccacaaacgc
    44161 agcatcgaca tcacgtgatc aattcgtaat gcgccgcagt tttgcatatt ggcacgcaac
    44221 agctcgataa acggttcata ggcacgcgcg gtgatgatat gcgggtccat tggcggcaat
    44281 ccccagttct gccccaacgg gccgaggata tccggcggcg cgccaaccga cgctttcagg
    44341 caatacagtt cacggtcgca ccaggtttct gccccacctt ctgctacacc aacggccaga
    44401 tcacgataca agccaatcgg catttcatag ccctggctta tctcccagca ggcggcaaac
    44461 tggctgtaag ccagccactg caaccagaga taaaaatcga cgtcatcacg atgttcttcg
    44521 cagaactggc gcacttctgg cgaatccacg ttctgataca tctctggcca tgctggccag
    44581 ccccagcgca tttcgtcctc tttcacttgc tgggcatgta gcgcatcaaa ggctgcctgc
    44641 cagaacaggc tgtcgccctg ctctgcaaca aactggcgaa acgcggtcat ctgctcatca
    44701 tcacgttgcg cgaaaccttt ccacgccatt cgtaatgctg tcatttttag ggcggtaacc
    44761 gtggagtaat caacccagtc ggcatcccgc gcctgttgca gcgtctgttg cgtggtcggc
    44821 aactgccacc aggcctgagc ctcttcgcta agatggaaat cttcaactgc gttaacgtcg
    44881 atataaatca cattcagcca acggcgagaa gacgggctgt acgggctggc actttccgga
    44941 tttgccagat agagcgcatg aatcgggttc agaccaataa acgacccgcc acgttttgcc
    45001 acatccacca gcatcgcttt gagatcgcca aaatccccaa taccccagtt tttttccgat
    45061 cgcagcgtat aaagctgaac gcaggcaccc cacagctttt gtttattcag caacgcctgc
    45121 ggttcgtaac agcgtttcgg ggcgacaatc acccggcaat gcgcgcgctg gtcgtcctgg
    45181 gtaagcgtca gcgtgtgata accttccggc agcttcgtcg gtagattgaa cgctttgccc
    45241 cccgtcacat ggcctttgta ctgctttcct tcttcggtgg tcagcagcca gctatattcg
    45301 ccgctgccct ccaccaccat cggcattttt ttgccgctgg tataaaccat gacattcggg
    45361 actggcgtta ccgccacttt cgtggcggta cgttgatgca tcgcgtcaag caaacgccgt
    45421 ttggtttcgg cgctaatcga ctgcggttta ccgtgggcat tgatgtaatt ggggctgatc
    45481 cccgccgcca gcgcggcatt atccagacgt ttgctttcca tcgagcttcc ttagcgtttt
    45541 gcctgccaga tacgagcctg ataatcgcga atagagcgat ccgagctaaa cataccgcag
    45601 cgggcggtat tgaggatcgc cgcgcgagtc caggcctcct ggtcgcggta cagcacatcc
    45661 acctgctttt gtgcctctac ataagcggcg aaatctgcca tcaccagata cggatcgccg
    45721 ccctgtttgc cgatactgtg cagcatctgg tcgaaggcat gcttatcgcc gtcgctgtat
    45781 ttaccgcttt ccaactcttt caatactgcg tccagcacct tatctttctt ccgccatttc
    45841 accgggtcgt agcctttggc cagaattgcc ttcacttgtt ccacggtatg accaaaaata
    45901 aagatatttt cttcaccgac tttctcagcg atttcaacgt tcgcaccatc cagcgtaccg
    45961 acagtaagcg caccattgag cgccagtttc atattgccgg taccggaagc ttctttacct
    46021 gcagtcgaaa tttgttcgga gatatccgcc gccgggatca gtttttccgc cgctgaaaca
    46081 caataatccg gcaagaacac caccttcaac ttgtcgccaa ccagcggatc gttgttgatc
    46141 acgtcagcca ctttgttgat cgcaaagata atatttttcg ccaggtagta gcccggtgct
    46201 gctttcgcgc cgaagaggaa gacgcgcggt acgcggtcag actgcgggtt ttcacgaatt
    46261 tctttgtaca acgccagaat atgcagcaga ttcagatgct ggcgtttgta ctcatgcaga
    46321 cgtttgatct gaatatcgaa aatcgcctgt ggattgatct caataccggt acgaactttc
    46381 acaaactctg ccagacggac tttattcgcc tgcttgatct cgcgatattg ctgacggaat
    46441 ttcgcatcat cagcgaattt ttccagattg atcagttgat cgagatcgtt agcccactct
    46501 tttttcagtg atttatccaa cagagccgcc agtgccgggt tgcactgttt gatccagcga
    46561 cgtggggtaa taccgttggt gacattatgg aatttgttcg gccatagctg gtgatattcc
    46621 gggaacagat ctttcaccac cagatctgag tgcagcgccg caacaccgtt caccgcgaaa
    46681 ccgccaacca cacacaggtt cgccatatgc acttgtttgt cgtgcaccac cgccagtttg
    46741 gcccacactt tttcatcgcc cggccaggtt ttctccacca gcgttttaaa gcgagtatta
    46801 atttcgttaa taatctgcat gtggcgcggc agtatgcctt tcaccagttt cacatcccag
    46861 cgttccagcg cttctggcat cagggtatgg ttggtgtaag cgaaagtttt gctggtgatg
    46921 gcccaggcgt catcccagct catctggtgc tcatcgatca gcacgcgcag cagttctgga
    46981 atggcgatag ttgggtgggt atcgttcagc tgaataactt cgtaatccgc cagttcgtgc
    47041 agtttacgcc ccgccagatg atggcgacgc aaaatatccg ctaccgagca ggcacactgg
    47101 aagtattgct gcatcaggcg cagcttttta ccggcagtat ggttgtcgtt tggatagaga
    47161 actttggtca gtttttccgc gttgatgccc tgctgttcgg cacgcaggaa atcaccgtcg
    47221 ttaaatttag tcagatcaaa cggatgcgca tgcgtcgcct gccacagacg cagcggctgc
    47281 gccacgccat tacgatagcc gacaacgggg agatcccacg cttgaccggt aatggtaaac
    47341 tccggctccc agcgtccgtc tttcgtcact ttaccgccaa tccctacctg cacatccagt
    47401 gcttcgttgt ggcggaacca cgggtagtta ctgcgatgcc agtcatccgg cgcctcaacc
    47461 tgtttgccat cgacaaaaga ctggcggaac aaaccatatt gatagttcag accgtaaccc
    47521 gtcgcagact gaccaacagt tgccattgag tcgaggaagc acgccgccag acgtcccaga
    47581 ccaccgttcc ccagcgccgg gtcgatctct tcttccagca agtcagtcag gttgatgtca
    47641 taagccttca acgaatcctg tacatcctga taccagccga gattcaacag attgttgccc
    47701 gtcaggcgac caatcaaaaa ctccattgag atgtagttaa catgccgctg attcgccacc
    47761 ggtttggcga atggctgagc acgcagcatt tcggccagtg cttcactcac tgccagccac
    47821 cactggcgag gagtcatttc agccgcagaa tttaagccat aacgctgcca ctgacgtgaa
    47881 agcgcttcct gaaattgctt atcgttaaaa ataggttgtg acataggagt tccacttttc
    47941 ttagattttc aacacaacgt tatcgctagt ttgccaggct cgatgttgac cttcctcatc
    48001 ctgcggggga ttaggcaggg aggagttgcg gggatgagca aggaaatgtg atctcgacca
    48061 cttaaagcta gtgcaaacca caggattagc agcaaatcaa tgctatacag cgcagaaaat
    48121 ctgtatctaa gtgcaaaaaa tggccgttgc gtattttcaa aaagcggaag gtaactctat
    48181 aaattaagta aaggggtgaa acagtttcac aagtaaaata tcaagtgtgc tccatctcat
    48241 tcttaataga tttattaaga tcatcttttt agatggcact ttcatcagaa atgaagaaca
    48301 aacccttgct taaatgaaac tgatgaacat aaggaaaata ccgtacaacc cagtattcac
    48361 gctggatcag cgtcgtttta ggtgagttgt taataaacat ttggaattgt gacacagtgc
    48421 aaattcagac acataaaaaa acgtcatcgc ttgcattaga aaggtttctg gccgacctta
    48481 taaccattaa ttacgaagcg caaaaaaaat aatatttcct cattttccac agtgaagtga
    48541 ttaactatgc tgattccgtc aaaattaagt cgtccggttc gactcgacca taccgtggtt
    48601 cgtgagcgcc tgctggctaa actttccggc gcgaacaact tccggctggc gctgatcaca
    48661 agtcctgcgg gctacggaaa gaccacgctc atttcccagt gggcggcagg caaaaacgat
    48721 atcggctggt actcgctgga tgaaggtgat aaccagcaag agcgtttcgc cagctatctc
    48781 attgccgcca tgcaacaggc aaccaacggt cactgcgcga tatgtgagac gatggcgcaa
    48841 aaacggcaat atgccagcct gacatcactc ttcgcccagc ttttcattga gctggcggaa
    48901 tggcatagcc cactttatct ggtcatcgat gactatcatc tgatcactaa tcctgtgatc
    48961 cacgagtcaa tgcgcttctt tattcgccat caaccagaaa atctcaccct tgtggtgttg
    49021 tcacgcaacc ttccgcaact gggcattgcc aatctgcgtg ttcgtgacca gctgctggaa
    49081 attggcagtc agcaactggc atttacccat caggaagcga agcagttttt tgattgccgt
    49141 ctgtcatcgc cgattgaagc tgcagaaagc agtcggattt gtgatgatgt ttccggttgg
    49201 gcgacggcac tgcagctaat cgccctctcc gcccggcaga atactcactc agcccataag
    49261 tcggcacgcc gcctggcggg aatcaatgcc agccatcttt cggattatct ggtcgatgag
    49321 gttttggata acgtcgatct cgcaacgcgc cattttctgt tgaaaagcgc cattttgcgc
    49381 tcaatgaacg atgcactcat cacccgtgtg accggcgaag aaaacgggca aatgcgcctc
    49441 gaagagattg agcgtcaggg gctgttttta cagcggatgg atgataccgg cgagtggttt
    49501 tgctatcacc cactgtttgg taacttcctg cgccagcgct gccagtggga actggcggcg
    49561 gagctgccgg aaatccaccg tgccgccgca gaaagctgga tggcccaggg atttcccagc
    49621 gaagcgattc atcatgcgct ggcggcaggc gatgcgctga tgctgcgcga tattctgctt
    49681 aatcacgcct ggagtttgtt caaccatagc gaactgtcgc tgctggaaga gtcgcttaag
    49741 gccctgccgt gggacagttt gctggaaaat ccgcagttgg tgttattgca ggcgtggctg
    49801 atgcaaagcc agcatcgcta cggcgaagtt aacaccctgc tggcccgcgc tgaacatgaa
    49861 atcaaggaca tcagagaagg caccatgcac gcagaattta acgctctgcg cgcccaggtg
    49921 gcgattaacg atggtagtcc ggatgaagcg gaacggctgg caaaactggc actggaagag
    49981 ctgccgccag gctggttcta tagccgcatt gtggcaacct cggtgctggg tgaagtgttg
    50041 cactgcaaag gcgaattgac ccgctcactg gcgttaatgc agcaaaccga acagatggca
    50101 cgccagcacg atgtctggca ctacgctttg tggagtttaa tccagcaaag tgaaattctg
    50161 tttgcccaag ggttcctgca aaccgcgtgg gaaacgcagg aaaaagcgtt ccagctgatc
    50221 aacgagcagc atctggaaca gctgccaatg catgagtttc tggtgcgcat tcgtgcgcag
    50281 ctgttatggg cctgggcacg gctggatgaa gccgaagcgt cggcgcgcag cgggattgaa
    50341 gtcttgtcgt cttatcagcc acagcaacag cttcagtgcc tggcaatgtt gattcaatgc
    50401 tcgctggccc gtggtgattt agataacgcc cgtagccagc tgaaccgtct ggaaaacctg
    50461 ctggggaatg gcaaatatca cagcgactgg atctctaacg ccaacaaagt ccgggtgatt
    50521 tactggcaaa tgaccggcga taaagccgcc gctgccaact ggttgcgtca tacggctaaa
    50581 ccggagtttg cgaacaacca cttcctgcaa ggtcaatggc gcaacattgc ccgcgcacaa
    50641 atcttgctgg gcgagtttga atctgcggaa attgtcctcg aagaactcaa tgaaaatgcc
    50701 cgcagtctgc ggttgatgag cgatctcaac cgtaacctgt tgctgcttaa tcaactgtac
    50761 tggcaggccg gacgtaaaag tgacgcccag cgtgtgttgc tggacgcatt aaaactggcg
    50821 aatcgtaccg gatttatcag tcattttgtc atcgaaggcg aagcgatggc gcaacaactg
    50881 cgccagctta ttcaactcaa tacgctgccg gagctggaac agcatcgcgc acagcgtatt
    50941 ctgcgagaaa tcaatcaaca tcatcggcat aaattcgccc atttcgatga gaatttcgtt
    51001 gaacgtctgc taaatcatcc ggaagtacca gagctgatcc gcaccagccc gttgacccaa
    51061 cgtgaatggc aggtcctggg gctgatttac tccggttaca gcaatgagca aattgcaggc
    51121 gagctggaag tggcggcaac caccatcaaa acgcatatcc gcaatctgta ccaaaaactc
    51181 ggtgtcgccc accgtcaggc cgcggtacaa cacgcgcaga aactgctgaa gatgatggga
    51241 tatggggtat gagtttagcc ggataatgcg ccagatccgg cttacatctt tacatcattc
    51301 aatgctcacc cgcgttacgc catctgtttc tatcagacta aaccgcactg gcaagaaacg
    51361 ctccaccacc gcgatattgg tctgcagatg gcatgaggga tgggcgaccg taaattctcc
    51421 cgcccccgcc agcgccatcg gtagcaccag ttggtcggcg agatattccc ccaccgccgc
    51481 cgggcttgcc aggtagcgtt tcacctcttt caccaactgt gccgcgacca cctcggcact
    51541 gacgcgcttt tcaccgacga caaaaaagcg ttcggtgata ttttcacttt cgacttcaag
    51601 cgagacggta ttacccggcc cctggtcgcg cggcaggtta tgaatattct gttcatgcaa
    51661 ggaaaaacta cccgccagtg tagcgatttc acgctcagca acatggcgcg gcacaccagc
    51721 cagtagcacc tctccacgca tctgcacaat gttcccgcgc tcgccaagtt gcaagctatt
    51781 aaacgatgcc accggtgaga cttccgttgc taccacgccg cctccggcag gataaaaacc
    51841 gtgacgtaac agcgtggttt gctgatgaac tcctattttc gccagcagcg gctccagcac
    51901 ccgacggata aaatccgcag gcggggccga cgggttatcg gtgccgccgc tcacttcaac
    51961 acgcgaaggt ccatcggcaa accacagcgc gggcagcacc gtttgcagca ccagcgtaca
    52021 acttccggcg ctaccgatag caaagcggta atcgccgccg cgcacggtgc caggtcggaa
    52081 gaccagacgc tgcgacccca gctcagcgcc ttccaccgtt gccctacaaa tttccgcagc
    52141 cgcttttacc gcggtcagat gttggcgcaa cagccccggt ttcgcccgcc cggcacgaat
    52201 gccggtgatg gtaaatggct ggccggttat catcgacagg ctcagcgccg agcgcaggat
    52261 ctgcccgccg ccttcgccct gtgcgccatc cagcgcaatc atccttttca tcatttatcc
    52321 ttttacgcac accacctgac gcagggtata gataacttcc accagatcgc tttgtgccgc
    52381 catcaccgca tcaatatctt tatacgccat cgggatttcg tcgatcacct cggcatcttt
    52441 acggcactcc acatgcgcgg tggcacggat ttgatcttcc acactgaaca gttttttcgc
    52501 tttagtacgg ctcattaccc gcccggcacc gtggctgcac gaacagaacg actcttcatt
    52561 tcccagccca cggacgataa agcttttcgc ccccatcgaa ccgggaataa ttccatattg
    52621 accagcacgc gcagacaccg cacctttacg cgtcacatag atctcttcgc caaagtgctg
    52681 ttctttttgc acatagttgt ggtgacagtt gatctcttcc atcgccagcg tttgtggctg
    52741 ttttaccgtt ttctgcgtaa cgctctgcaa cgccgttacc acgttttcca tcatcgcatc
    52801 gcggttaagg ctggcaaaaa gctgcgccca ggccacggct ttcaggtaat catcaaagta
    52861 ttccgtacct tccataaagt acgccaggtc acgcgatggc aacgtttcaa gcgtttcctg
    52921 catctctttt tgcgccagat cgataaaata agttccgatg gcgttaccaa ttccgcgtga
    52981 accggagtgc agcataatcc acacctggtc cgactcatca aggcagattt caataaagtg
    53041 gttacccgtt cccagcgttc ccaggtgttt atagttgtta gtgttaagga aacggggata
    53101 tttttgcgtt aaccactgat aaccggcttc cagctcagcc catttcgcat cgacgttaac
    53161 aggcggattt tcccatgcgc ctttatcacg tttacaacgc ccggtagtac gtccgtgcgg
    53221 tacggccgtt tcaatcgcct gacgcagctc tgccaggttt tcaggcaggt ccgccgccgt
    53281 taacgcggta cgcagtgcgt tcattccaca gccaatatcc acgcccaccg ccgccggaat
    53341 aatcgcccct ttggtcggga tcacgctacc aatggtggaa cctttaccca ggtgtacatc
    53401 aggcattacc gcaatatgtt tgaaaataaa tggcatcttc gcggtattaa taagttgctg
    53461 acgcgcatcg gcttctaccg gcacgccttt ggtccacatt tttaccggag cattttcagt
    53521 ggtcagtaat tcgtaattca ttttgttttt ctcttttcgt tgtttgctgt cctgataatt
    53581 gcaaccgtcg tgccagaaaa tcaaataagc atcttaattt tttaattcat tgtttttaaa
    53641 aatattatat ttttacgtcc gtaaacggag atttcccgca aagccgattt accgataatg
    53701 aaatctcgtc ttttataagg atatctaaga tgcgtaaaac agtggctttt ggctttgtcg
    53761 gaacggtgct ggattatgcc gggcgcggca gtcagcgctg gtcgaaatgg cgcccgacac
    53821 tctgtttatg tcagcaagaa tctttggtgg tcgatcgact ggaattattg cacgacgccc
    53881 gctcgcgctc gctgttcgaa acgcttaaac gcgatatcgc cagcgtttcg ccagaaacaa
    53941 aagtggtggg cgttgagatt gaactgcata acccgtggga tttcgaagaa gtttacgcct
    54001 gcctgcatga tttcgcccgt ggttacgcgt tccaacctga taaagaagag tatttaattc
    54061 acatcaccac cggcacccac gtcgcgcaga tttgctggtt tctgctggca gaagcgcgtt
    54121 acctgcccac ccggttgata caatcttcac caccgcgcaa aaaagagcgg cccgatagcc
    54181 ctggcgcagt gacgattatc gatctcgatt taagccgtta taacgccatc gccagccgct
    54241 ttgccgagga acgccagcaa acgcttgatt ttcttaagtc cggtattgcc acgcgtaact
    54301 cccacttcaa ccgcatgatt gagcagattg aaaaagtggc gataaaatcc cgcgcaccga
    54361 tcctgcttaa cggcccgacc ggtgcaggta agtcatttct ggcacgacgt atcttcgagc
    54421 taaaacaggc gcggcatcag tttagcggcg cgtttgtgga agtgaactgc gccaccctgc
    54481 gcggcgatac cgccatgtcg acgctgtttg gtcatgtgaa aggcgcgttt accggggcgc
    54541 gggaatcgcg tgaaggatta ttacgcagcg ccaacggcgg aatgttgttt cttgatgaga
    54601 ttggcgaact gggcgcagac gaacaggcaa tgctgctgaa agccattgaa gagaaaacct
    54661 tttacccgtt tggcagcgat cgccaggtga gcagtgattt tcagcttatc gccggaacgg
    54721 tgcgtgattt gcgccagttg gttgccgaag gcaaatttcg tgaagacctg tacgcgcgaa
    54781 taaatctctg gaccttcacc ttgccaggat tacgccaacg ccaggaagat attgaaccga
    54841 acctggatta tgaagtggag cgccacgcct cactcactgg cgacagcgtg cgttttaaca
    54901 ccgaagcgcg gcgtgcctgg ctggcctttg cgacctctcc ccaggcaaca tggcgcggta
    54961 actttcgcga actttctgcc agcgtcacgc ggatggccac ctttgccact agcggacgca
    55021 tcacactgga tacggtggaa gatgagatca accgtctgcg ctataactgg caggagagtc
    55081 gcccctccac gcttacggcg ttgctgggcg cagaggcaga aaacatcgat ctcttcgacc
    55141 gtatgcaact ggaacacgtt atcgctatct gccgccaggc aaagtcgctt tctgctgccg
    55201 gacgtgaact gtttgacatt tcgcgccagg gtaaagccag cgtcaatgac gcggatcggc
    55261 tacgcaaata cctggcgcgt tttggtctga catgggaaac aatgcaggat cagcacagct
    55321 ccagttgaat atgatggtcc ttcagcacct gcatcacgct cgcaggcggc atcgtgtcgg
    55381 tgtagacagc atcgaccatg ctgatgctac ccatattgac cattgcgtta cggccaaatt
    55441 ttgagtggtc gacaaccagc ataacgtggc gcgagttctc aatgatggcg cgtttggtgc
    55501 gaacttcgtg gtaatcgaac tccagtagcg agccgtcgct atcgatgccg cttatcccca
    55561 gaatgccgaa atcaaggcgg aactgggaga taaaatcgag cgttgcttcg ccaatgatcc
    55621 cgccatcacg gctgcgtaat tcgccaccgg caagaatgat acggaaatcc tcttttacca
    55681 tcagcgtgtt agcgacgttg agattgttgg tcacaatgcg taaattgctg tgattaagca
    55741 gcgcgtgcgc caccgcttcc ggcgtggtgc cgatatcgat aaacagcgtc gagccattgg
    55801 gaatttgctc cgccactttg cgggcgatgc gctctttttc ttcggtctgg gtggccttgc
    55861 gatcgtgcca cggcgtgtta accgaactgg aaggcagcgc cgcaccgcca tgatggcgca
    55921 ggatcaggtt ttgctctgcc agctcgttga gatcgcggcg aatagtctgc gggctaacgg
    55981 agaaatgctc taccagttcc tcggtgctga cataaccctg ctgtttaacc agttcgataa
    56041 taccgttgtg acgttgtgtt tgtttcattg ataaatccct ggaattattt tcgttttcgc
    56101 gcattgagcg aatcaacaaa agccatcgct aaacccacgg ctaacccggc gatgtgtgct
    56161 ccgttcgcca tcgacatccc aaacaaatca aaccatccgg cgacaatcca gatcaacgca
    56221 aagataatta acccacgttg caggtaaatg ccactttgcg gatcgcgttc gccacgtagc
    56281 cagacgtagc ccatcagcgc atacaccacg ccagaaagcc cgccaaacca cggcccgctg
    56341 aatttttgct gcacatagcc gcttaacagg gcgctaatga gagtaatgac aattagctta
    56401 ccgctaccga ggcgtttttc caccgcaccg ccgagatacc accaccagag caggttaaag
    56461 aggatatgca tcagcgagaa gtgcattaac gcgtgggtga agtaacgcca gaactcaaat
    56521 ttcagcgtcg gatcgaatgg ccaggccagc cataacatca cttcttgatc gccgagaatt
    56581 tgcatggcga taaacaccac cacgcaggcg atcatcatca cccaggttac cggacctgcg
    56641 cgttcacgca aggcggcaaa gaaaggataa cggcgataat gcaggccact gccggtatgg
    56701 cccgcctgcc agctggccgc cagataacgc ggatctgccg ggttttcgag aaaacgcgcc
    56761 agctccgccc gcacgcgctc ggcctgggac tcatccgcca gccagacatc gctttggtta
    56821 tgttgttgaa tcgtgaggat aacaccctgc gtcgccatgt aatcaacaaa cgcctgcgcc
    56881 acgcgggggt tagcaaaaga ggtaatcatc aacatcgttg ctgtcgctta ttccacacaa
    56941 aaggggacag tataaagcgt tacgcgccgt acgccacctc tgcgggaaac tggcgctgcc
    57001 aggcttcaaa accgccatca atgctgtaga ccacatcgta gccctgttgc agcagatact
    57061 gcgccgcgcc tttgctgcta ttgccgtgat aacacatcac catcaccggt gtgtcaaagt
    57121 cgttatcacg cataaacgcg cccagtgtgt cgttagttaa atggaaagcc tgggtcgcat
    57181 gccccattgc gaaactctgt ggatcgcgaa tatcgaccag caccgcctct ttttcctgca
    57241 acttctggtg cgcgtcggta acgttaatac attcgaactg atccatgcgt ctctctttct
    57301 ttacaaacaa gtgggcaaat ttaacgcaca gtttacgtcg aagcggcaga taaacgccat
    57361 aatgttatac atatcactct aaaatgtttt ttcaatgtta cccaaagcgc gattctttgc
    57421 taatatgttc gataacgaac atttatgaac tttaacgaaa gtgaatgagg gcagcatgga
    57481 aaccaaagat ctgattgtga taggtggcgg catcaatggt gctggtatcg cggcagacgc
    57541 cgctggacgc ggtttatccg tgctgatgct ggaggcgcag gatctcgctt gcgcgacctc
    57601 ttccgccagt tcaaaactca ttcacggtgg cctgcgctac cttgagcact atgaattccg
    57661 actggtcagc gaggcgctgg ctgaacgtga agtgctactg aaaatggccc cgcatatcgc
    57721 cttcccgatg cgttttcgcc tgccgcatcg tccgcatcta cgcccggcgt ggatgattcg
    57781 tattggtctg tttatgtacg atcatctggg taaacgcacc agcctgccgg gatcaaccgg
    57841 tttgcgtttt ggcgcaaact cagtgttaaa accggaaatt aagcgcggat tcgaatattc
    57901 tgactgttgg gtagacgacg cccgtctggt actcgccaac gcccagatgg tggtgcgtaa
    57961 aggcggtgaa gtgcttactc gtactcgtgc cacatctgct cgccgcgaaa acggcctgtg
    58021 gattgtggag gcggaagata tcgataccgg caaaaaatat acatggcagg cgcgcggcct
    58081 ggttaatgct accggcccgt gggtgaaaca atttttcgac gacgggatgc atctgccttc
    58141 gccttatggc attcgcctga tcaaaggcag ccatattgtg gtgccgcgtg tgcataccca
    58201 gaagcaagcc tacattctgc aaaacgaaga taaacgtatt gtcttcgtga tcccgtggat
    58261 ggacgagttt tccatcatcg gcaccaccga tgtggaatac aaaggcgatc caaaagcagt
    58321 gaagattgaa gagagtgaaa tcaattacct gttgaaagtg tataacacgc actttaaaaa
    58381 gcagttaagc cgggacgata tcatctggac ctactccggc gtgcgtccgc tatgtgatga
    58441 tgagtccgac tcgccacagg ccattacccg tgattacacc cttgatatcc atgatgaaga
    58501 cggcaaagcc ccgctgctgt cggtattcgg cggtaagctg accacctacc gtaaactggc
    58561 ggagcacgcg ctggaaaaac tgacgccata ttatcagggc attggtccgg cgtggacgaa
    58621 agagagcgtg ctgccgggtg gtgccattga aggcgaccgt gacgattacg ccgcccgcct
    58681 gcgccgccgt tacccgttcc tgaccgaatc gctggcacgt cactacgcgc gcacttacgg
    58741 cagcaacagc gaactgttgc tcggcaatgc aggggcgata agcgacctcg gtgaagattt
    58801 cggtcatgag ttctacgaag cggagctgaa atatctggtc gaccacgaat gggtccgccg
    58861 cgccgacgat gccctgtggc gtcgcacgaa acaaggcatg tggctaaatg cggatcaaca
    58921 atctcgtgtg agtcagtggc tgatggaata tacgcagcag aggttgtcgc tggcgtcgta
    58981 aggatgatgc ccggttgcga agtgcaaccg ggctatttga attccttaga ctattcattg
    59041 caaataattg aataacggcc ccagagctaa aatcgccaaa cttaagcacg ttacccaatc
    59101 gccccacttc ttcctatggc ttctattttt gctacgccac tggcaataat atcccaacgc
    59161 aaaaccaagc tgtgcactaa tcgggccgac aaaatcagtc cacggctgcg gccaaaaagg
    59221 ggaaaaattc aggggaagcc agacaaacgt cagaaagaaa tttcggatat tttccagcaa
    59281 cgctgatttc tctggagaaa tataaagaat gaaattcgag agaattaacc agaaatagat
    59341 cggagaaaag accagccagc aatgtagcct gttttttaat tcaggaggcg ttctgcggga
    59401 gaattgccaa ccgatgccac acattacact gaaaaaaagt gcatattcaa ccaataactc
    59461 aagttgtaat tcctgaagag aaatacgcac aaccaggagc ggaaaggaga atattatttc
    59521 cgccagaaaa gttaacgtga atataagtac ggctaaggca ataaccgccg gccataccaa
    59581 taatggtacg ataacaaaac cgaaaattac agggtaaata taaagccgga atttattcat
    59641 ctttattgtc ctgatgattc ggatgatgcg tcatacatgg cacataaaca ctcatcaatc
    59701 atccagtcgt cttcccagat ctgatactgt gtgccctgtt cgggctgctt aagcgggttc
    59761 tcaatcgcct gccgcgcgcg ttctttcagg atggattgat tgttgaaggc ccaggcattg
    59821 atttgggcgg taatggcttt atcgtcatca tcctgctcaa aacgactatt ctgaatattc
    59881 tcttccctgt tatgccacag caaacatgcc atcgatactg tgggttggta accgggtaca
    59941 gagatgacgg catcaagcaa tggcgaaaga gcgatatcgg cacgggaaaa catttccagc
    60001 atatactgta attcgacagc ctcatcgcca gatacccaat cagccatata ctggcaggca
    60061 aatcgctgca atattgcccg ttcatcctca cgccagtttt ctttactggc aaggttgata
    60121 cgccgtagag aaaatacttc gtgaatattg tcaaattgcc tctgcgcaac aaactccagt
    60181 aaacgaggaa gaaaataacg aacctcatcg ctgttttgcg gatcaggtcc tggactacta
    60241 ttccaggcat tgattaagga atacgggatc gctcgtaacg atgtattccg taatgccttt
    60301 tgttcctgct gactcaggca atattcacaa caaaccacaa attgctgcgg caccggataa
    60361 cgacgaaaaa tatgataaac ctgctcaatt aatgcctgca tggcggggct gggttgcata
    60421 attacccctc gcccgctgat cgcgtgtaaa cggttgataa acaaaacgag cattcgccgc
    60481 attcaggcga ataatggcac tacgagagag ggaaaagctg ggccgaatgc aataattaca
    60541 gtcccaacag ctttcctctg tgcaattttc cactgagtaa tgacgcgcgt agagtttcag
    60601 ttcagcctgc ttatcgcgaa atccgcgtaa actgttaccg ttttgaaatt gccaggcttc
    60661 tttaaacacc tgaaagatat aaacatcctc aatccaggcg tcacattttt taagcggacg
    60721 ctttgctatg atcacccgct catccgggtt gagcatcgcc gtaaattgta cggtgaagtc
    60781 atttgtcgtg ttcagatcac attgaacatc tggtacatca ctgatataga ggtcaaatcc
    60841 aaagtcagca ccacaggcac aaagacggct aatggtggcg gtatcgaggg aaaatcccgg
    60901 ccatacattg ctggacgtta aaccaatatt taatgtgaca tcatatcgag ataatccctc
    60961 aagaccggca gcaccggcga acttttcccc ttcatccagg acctggttaa tgagttgctg
    61021 aacaatgcct tcatcccagt atgttgattt gcagttggca atcgtaatat ccacgccagt
    61081 gaatggttca gtaccggaga ggcttgcaaa taacgtatca actaattcca tttttattat
    61141 ccagcttaat gcaacatttt atccatcggc acataaccta cattttccac caggtttttc
    61201 ccctgtagaa ttaaaaatat ttttttgttt tgcattccgt tcatccctat tattgcattt
    61261 tattttgcgg tgatggctaa accgattctg taccagctca aaaattactg atattccact
    61321 cgcaatatag ccagtgcatt ataacgacct gccgctggca cttttccagt tgtactaaca
    61381 ggtaaagtca ggaaagtaac atcagcagtt tgtgcagaag cagcctggtt ttccaccaat
    61441 ctgaacggaa cgcctactga tgttgccaga gcatttaaat ctacggagtt ttgctgacta
    61501 tccataatca taataccaac atcaggatta tcctgtgcct gaataatact ttgtttccca
    61561 ttggtatcac gtgtggcgga aatgttaccg tcgaatacca acgtcattac cgcatctgta
    61621 tctttattcg tacaattaat cggaatagtt gttgctacag gagtaacacc agcgggcttc
    61681 gctccggcac cagcccggct caattcactg gcccacacat ccggcatatt aacagtaaaa
    61741 ctcgtaccct cattaatagt gcaaccttgc ggaacctgaa tatttaagtt tcctatgtag
    61801 gcattaaaga cagggatagg accatgttcg gtagggatct ggctgacata taatgacacc
    61861 aataatacgt tacttaatga aacatcagat ataatttttt tatcaatcct gattttaata
    61921 tatccttcag tatctgatgc ccaattagat ggtttgcttc tgtcccccgg ataattggtg
    61981 ttataatttt cgaatggcac aattttatct gtcattgtgc cattctgatt ataaagtttt
    62041 atatgagtag agacagatat ttcatgggtg agtggaataa atacatcact cccttgatga
    62101 tcaccactgg caccagacgg atctattcca gagacagcac tgaagtaaat ctcataatca
    62161 tcaagagaat tacaataagc cttataggtt ccaaccttcc agatttgtga tggcaataca
    62221 gtgccaggcg catttttgtt gacatcagaa accactatag taccaaaatt ataagaaaag
    62281 gatgcaggcg tagtattatt actcggtgaa cccggataac aatatgcacg ttctcgcccc
    62341 atatcttcag cattagcaat agaagaaaga acatttgaaa atataataat caat
//
LOCUS       DADSYM010000005        58847 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2415
            SAMN08039579-rid11723173.denovo.005, whole genome shotgun sequence.
ACCESSION   DADSYM010000005 DADSYM010000000
VERSION     DADSYM010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039579
            Sequence Read Archive: SRR6362533
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 58847)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 58847)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980162.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 03:39:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,484
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 4,347
            CDSs (with protein)               :: 4,347
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 43 of 85
            Pseudo Genes (incomplete)         :: 37 of 85
            Pseudo Genes (internal stop)      :: 19 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..58847
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039579-rid11723173.denovo.005"
                     /strain="LGE2415"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8302 N 95.9628 W"
                     /collection_date="2015-08-24"
     gene            complement(<1..194)
                     /locus_tag="J1Y83_000246"
     CDS             complement(<1..194)
                     /locus_tag="J1Y83_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001592478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA7628435.1"
                     /translation="MALNCPTCKTPLERNGETAHCATCDKDFTVQALCPECHQALQVL
                     KACGAVDYFCQHGHGLISKKR"
     gene            complement(257..1729)
                     /gene="der"
                     /locus_tag="J1Y83_000247"
     CDS             complement(257..1729)
                     /gene="der"
                     /locus_tag="J1Y83_000247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HBA7628436.1"
                     /translation="MVPVVALVGRPNVGKSTLFNRLTRTRDALVADFPGLTRDRKYGR
                     AEIEGREFICIDTGGIDGTEDGVETRMAEQSLLAIEEADVVLFMVDARSGLMPADEAI
                     AKHLRSREKPTFLVANKTDGLDPDQAVVDFYALGLGEIYPIAASHGRGVLSLLEHVLL
                     PWMEDLAPQEEVDEDAEYWAQFEAEENGEEEEEDDFDPQSLPIKLAIVGRPNVGKSTL
                     TNRILGEERVVVYDMPGTTRDSIYIPMERDGREYVLIDTAGVRKRGKITDAVEKFSVI
                     KTLQAIEDANVVMLVIDAREGISDQDLSLLGFILNSGRSLVIVVNKWDGLSQEVKEQV
                     KETLDFRLGFIDFARVHFISALHGSGVGNLFESVREAYDSSTRRVGTSMLTRIMTMAV
                     EDHQPPLVRGRRVKLKYAHAGGYNPPIVVIHGNQVKDLPDSYKRYLMNYFRKSLDVMG
                     SPIRIQFKEGENPYANKRNTLTPTQMRKRKRLMKHIKKSK"
     gene            complement(1847..3025)
                     /gene="bamB"
                     /locus_tag="J1Y83_000248"
     CDS             complement(1847..3025)
                     /gene="bamB"
                     /locus_tag="J1Y83_000248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamB"
                     /protein_id="HBA7628437.1"
                     /translation="MQLRKLLLPGLLSVTLLSGCSLFNSEEDVVKMSPLPTVENQFTP
                     TTAWSTSVGSGIGNFYSNLHPALADNVVYAADRAGLVKALNADDGKEIWSVNLAEKDG
                     WFSKDPALLSGGVTVSGGHVYIGTEKAQVYALNTSDGTVAWQTKVAGEALSRPVVSDG
                     LVLIHTSNGQLQALNEADGAVKWTVNLDMPSLSLRGESAPATAFGAAVVGGDNGRVSA
                     VLMEQGQMIWQQRISQATGSTEIDRLSDVDTTPVVVNGVVFALAYNGNLTALDLRSGQ
                     IMWKRELGSVNDFIVDGNRIYLVDQNDRVMALTIDGGVTLWTQSDLLHRLLTSPVLYN
                     GNLVVGDSEGYLHWINVEDGRFVAQQKVDSSGFQTEPVAADGKLLIQAKDGTVYSITR
                     "
     gene            complement(3036..3656)
                     /locus_tag="J1Y83_000249"
     CDS             complement(3036..3656)
                     /locus_tag="J1Y83_000249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfgM family protein"
                     /protein_id="HBA7628438.1"
                     /translation="MEIYENENDQVEAVKRFFAENGKALAVGVILGVGALIGWRYWNS
                     HQVDSARSASLAYQNAVTAVSEGKPDSIPAAEKFAAENKNTYGALASLELAQQFVDKN
                     ELEKAAAQLQQGLADTSDENLKAVINLRLARVQVQLKQADAALKTLDAIKGEGWAAIV
                     ADLRGEALLSKGDKQGARSAWEAGVKSDVTPALSEMMQMKINNLSI"
     gene            complement(3674..4948)
                     /gene="hisS"
                     /locus_tag="J1Y83_000250"
     CDS             complement(3674..4948)
                     /gene="hisS"
                     /locus_tag="J1Y83_000250"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HBA7628439.1"
                     /translation="MAKNIQAIRGMNDYLPGETAIWQRIEGTLKNVLGSYGYSEIRLP
                     IVEQTPLFKRAIGEVTDVVEKEMYTFEDRNGDSLTLRPEGTAGCVRAGIEHGLLYNQE
                     QRLWYIGPMFRHERPQKGRYRQFHQLGCEVFGLQGPDIDAELIMLTARWWRALGISEH
                     VTLELNSIGSLEARANYRDALVAFLEQHKEKLDEDCKRRMYTNPLRVLDSKNPEVQVL
                     LNDAPALGDYLDEESREHFAGLCKLLESAGIAYTVNQRLVRGLDYYNRTVFEWVTNSL
                     GSQGTVCAGGRYDGLVEQLGGRATPAVGFAMGLERLVLLVQAVNPEFKADPVVDIYLV
                     ASGADTQSAAMALAERLRDELPGVKLMTNHGGGNFKKQFARADKWGARVAVVLGESEV
                     ANGTAVVKDLRSGEQTAVAQDSVAAHLRTLLG"
     gene            complement(5059..6177)
                     /gene="ispG"
                     /locus_tag="J1Y83_000251"
                     /gene_synonym="gcpE"
     CDS             complement(5059..6177)
                     /gene="ispG"
                     /locus_tag="J1Y83_000251"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HBA7628440.1"
                     /translation="MHNQAPIQRRKSTRIYVGNVPIGDGAPIAVQSMTNTRTTDVEAT
                     VNQIKALERVGADIVRVSVPTMDAAEAFKLIKQQVNVPLVADIHFDYRIALKVAEYGV
                     DCLRINPGNIGNEERIRMVVDCARDKNIPIRIGVNAGSLEKDLQEKYGEPTPQALLES
                     AMRHVDHLDRLNFDQFKVSVKASDVFLAVESYRLLAKQIDQPLHLGITEAGGARSGAV
                     KSAIGLGLLLSEGIGDTLRVSLAADPVEEIKVGFDILKSLRIRSRGINFIACPTCSRQ
                     EFDVIGTVNALEQRLEDIITPMDVSIIGCVVNGPGEALVSTLGVTGGNKKSGLYEDGV
                     RKDRLDNNDMIDQLEARIRAKASQLDEARRIDVQQVEK"
     gene            complement(6204..7211)
                     /gene="rodZ"
                     /locus_tag="J1Y83_000252"
     CDS             complement(6204..7211)
                     /gene="rodZ"
                     /locus_tag="J1Y83_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytoskeleton protein RodZ"
                     /protein_id="HBA7628441.1"
                     /translation="MNTEATHDQNEALTTGARLRNAREQLGLSQQAVAERLCLKVSTV
                     RDIEEDKAPADLASTFLRGYIRSYARLVHIPEEELLPGLEKQAPLRAAKVAPMQSFSL
                     GKRRKKRDGWLMTFTWLVLFVVIGLSGAWWWQDHKAQQEEITTMADQSSAELNNNQSQ
                     SVPLDTSTTTDQAMATTPTSPVDTTATNTQTPAVTAPAPAVDPQQNAVVPPSQANVDT
                     AATPAPAATTTPDGAAPLPTDQAGVTTPAVDPNALVMNFTADCWLEVTDATGKKLFSG
                     MQRKDGNLNLTGQAPYKLKIGAPAAVQIQYQGKPVDLSRFIRTNQVARLTLNAEQSPA
                     Q"
     gene            complement(7496..8650)
                     /gene="trmG/rlmN"
                     /locus_tag="J1Y83_000253"
     CDS             complement(7496..8650)
                     /gene="trmG/rlmN"
                     /locus_tag="J1Y83_000253"
                     /EC_number="2.1.1.-"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA
                     (adenosine(37)-C2)-methyltransferase TrmG/ribosomal RNA
                     large subunit methyltransferase RlmN"
                     /protein_id="HBA7628442.1"
                     /translation="MSEQLVTPENVTTKDGKINLLDLNRQQMREFFKDLGEKPFRADQ
                     VMKWMYHYCCDNFDEMTDINKVLRGKLKEVAEIRAPEVVEEQRSSDGTIKWAIAVGDQ
                     RVETVYIPEDDRATLCVSSQVGCALECKFCSTAQQGFNRNLRVSEIIGQVWRAAKIVG
                     AAKVTGQRPITNVVMMGMGEPLLNLNNVVPAMEIMLDDFGFGLSKRRVTLSTSGVVPA
                     LDKLGDMIDVALAISLHAPNDEIRDEIVPINKKYNIETFLAAVRRYLEKSNANQGRVT
                     IEYVMLDHVNDGTEHAHQLAELLKDTPCKINLIPWNPFPGAPYGRSSNSRIDRFSKVL
                     MSYGFTTIVRKTRGDDIDAACGQLAGDVIDRTKRTLRKRMQGEAIDIKAV"
     gene            complement(8800..9231)
                     /gene="ndk"
                     /locus_tag="J1Y83_000254"
     CDS             complement(8800..9231)
                     /gene="ndk"
                     /locus_tag="J1Y83_000254"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HBA7628443.1"
                     /translation="MAIERTFSIIKPNAVAKNVIGSIFARFEAAGFKIVGTKMLHLTV
                     EQARGFYAEHDGKPFFDGLVEFMTSGPIVVSVLEGENAVQRHRDLLGATNPANALAGT
                     LRADYADSLTENGTHGSDSVESAAREIAYFFGEGEVCPRTR"
     gene            complement(9380..11692)
                     /gene="pbpC"
                     /locus_tag="J1Y83_000255"
     CDS             complement(9380..11692)
                     /gene="pbpC"
                     /locus_tag="J1Y83_000255"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417014.1"
                     /note="penicillin-binding protein 1C; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase PbpC"
                     /protein_id="HBA7628444.1"
                     /translation="MPRLLTKRGCWITLAAAPFLLFLAAWGADKLWPLPLHEVNPARV
                     VVAQDGTPLWRFADADGIWRYPVTIEDVSPRYLEALINYEDRWFWMHPGVNPFSVARA
                     AWQDLTSGRVISGGSTLTMQVARLLDPHPKTFGGKIRQLWRALQLEWHLSKREILTLY
                     LNRAPFGGTLQGIGAASWAYLGKSPANLSYSEAAMLAVLPQAPSRLRPDRWPERAEAA
                     RNKVLERMAVQGVWSREQVKESREEPIWLAPRQMPQLAPLFSRMMLGKSKSDKIVTTL
                     DAGLQRRLEELAQNWKGRLPPRSSLAMIVVDHTDMRVRGWVGSVDLNDDSRFGHVDMV
                     NAIRSPGSVLKPFVYGLALDEGLIHPASLLQDVPRRTGDYRPGNFDSGFHGPISMSEA
                     LVRSLNLPAVQVLEAYGPKRFAAKLRNVGLPLYLPNGAAPNLSLILGGAGAKLEDMAA
                     AYTAFARHGKAGKLRLQPDDPLFERPLMSSGAAWIIRRIMADEAQPLPDGDLPRVAPL
                     AWKTGTSYGYRDAWAIGVNARYVIGIWTGRPDGTPVVGQFGFASAVPLLNQVNNILLS
                     RSVNLPEDPRPDSVSRGVICWPGGQSLPEGDGNCRRRLATWLLDGSQPPTLLLPEQEG
                     INGIRFPIWLDENGKRVAADCPQARQEMINVWPLPLEPWLPASERRAVRLPPASTICP
                     PYGHDAQLPLQLTGVRDGAIIKRLPGAAEATLPLQSSGGAGERWWFLNGEPLTERGRN
                     VTLHLTDKGDYQLLVMDDVGQIATVKFVMQ"
     gene            complement(11693..16654)
                     /gene="yfhM"
                     /locus_tag="J1Y83_000256"
     CDS             complement(11693..16654)
                     /gene="yfhM"
                     /locus_tag="J1Y83_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha2-macroglobulin"
                     /protein_id="HBA7628445.1"
                     /translation="MKKLRVAACMLMLALAGCDNNDNAPTAVKKDAPSEVTKAASSEN
                     VSSAKLSAPERQKLAQQSAGKALTLLDLSEVQLDGAATLVLTFSIPLDPDQEFSRVIH
                     VVDKKSGKVDGAWELSDNLKELRLRHLEPKRDLIVTIGKEVKALNNATFSKDYEKTIT
                     TRDIQPSVGFASRGSLLPGKVIEGLPVMALNVNNVDVNFFRVKPESLPAFISQWEYRN
                     SLANWQSDKLLQMADLVYTGRFDLNPARNTREKLLLPLGDIKPLQQAGVYLAVMNQAG
                     RYDYSNPATLFTLSDIGVSAHRYHNRLDIFTQSLENGAAQQGVEVSLLNEKGQTLTQA
                     TSDAQGHVQLENDKNAALLLARKDGQTTLLDLKLPALDLAEFNIAGAPGYSKQFFMFG
                     PRDLYRPGETVILNGLLRDADGKALPDQPIKLDVIKPDGQVLRSVVSQPENGLYHFTW
                     PLDSNAATGMWHIRANTGDNQYRMWDFHVEDFMPERMALNLTGEKTPLTPKDEVKFSV
                     VGYYLYGAPANGNTLQGQLFLRPLREAVSALPGFEFGDIAAENLSRTLDEVQLTLDDK
                     GRGEVSTESQWKETHSPLQVIFQGSLLESGGRPVTRRAEQAIWPADALPGIRPQFASK
                     SVYDYRTDSTVKQPIVDEGSNAAFDIVYSDAQGVKKAVSGLQVRLIRERRDYYWNWSE
                     YEGWQSQFDQKDLIENEQTLDLKADETGKVSFPVEWGAYRLEVKAPNEAVSSVRFWAG
                     YSWQDNSDGGGAVRPDRVTLKLDKASYRPGDTIKLHIAAPTAGKGYAMVESSEGPLWW
                     QEIDVPAQGLDLTIPVDKTWNRHDLYLSTLVVRPGDKSRSATPKRAVGVLHLPLGDEN
                     RRLDLALETPTKMRPNQPLTVKIKASNKNGEMPKQVNVLVSAVDSGVLNITDYVTPDP
                     WQAFFGQKRYGADIYDIYGQVIEGQGRLAALRFGGDGDELKRGGKPPVNHVNIVAQQA
                     LPVTLNEQGEGSVTLPIGDFNGELRVMAQAWTADDFGSNESKVIVAAPVIAELNMPRF
                     MASGDTSRLTLDITNLTDKPQKLNVALTASGLLELVSNSPAPVELAPGVRTTLFIPVR
                     ALPGYGDGDIQATISGLALPGETVADQHKQWKIGVRPAFPAQTVNYGTALQPGETWAL
                     PADGLQNFSPVTLEGQLLLSGKPPLNIARYIKELKAYPYGCLEQTASGLFPSLYTNAA
                     QLQALGIKGDSDEKRRASVDIGISRLLQMQRDNGGFALWDKNGDEEYWLTAYVMDFLV
                     RAGEQGYSVPTDAINRGNERLLRYLQDPGMMSIPYADNLKASKFAVQSYAALLLARQQ
                     KAPLGALREIWEHRADAASGLPLLQLGVALKTMGDAMRGEEAIVLALKTPRNSDERIW
                     LGDYGSPLRDSALMLSLLEENKLLPDEQYSLLNTLSQQAFGERWLSTQESNALFLAAR
                     TLQDLPGKWQAQTTFSAEPLTGEKAQTSNLNSDQLATLQVTNSGDQPLWLRVDASGYP
                     QSAPLPASNVLQIERHILGTDGKSKSLDSLRSGDLVLVWLQVKASNSVPDALVVDLLP
                     AGLELENQNLANGSASLEQSGGEVQNLLNQMQQASIKHIEFRDDRFVAAVAVDEYQPV
                     TLVYLARAVTPGTYQVPQPMVESMYVPQWRATGAAEDLLIVRP"
     gene            16861..17706
                     /gene="sseA"
                     /locus_tag="J1Y83_000257"
     CDS             16861..17706
                     /gene="sseA"
                     /locus_tag="J1Y83_000257"
                     /EC_number="2.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-mercaptopyruvate sulfurtransferase"
                     /protein_id="HBA7628446.1"
                     /translation="MSTTWFVGADWLAEHIDDPEIQIIDARMASPGQEDRNVAQEYLN
                     GHIPGAVFFDIEALSDHTSPLPHMLPRPETFAVAMRELGVNQDKHLIVYDEGNLFSAP
                     RAWWMLRTFGVEKVSILGGGLAGWQRDDLLLEEGAVELPEGEFNAAFNPEAVVKVTDV
                     LLASHENTAQIIDARPATRFNAEVDEPRPGLRRGHIPGALNVPWTELVREGELKTTDE
                     LDAIFFGRGVSYDKPIIVSCGSGVTAAVVLLALATLDVTNVKLYDGAWSEWGARADLP
                     VEPVK"
     gene            complement(18199..18975)
                     /gene="sseB"
                     /locus_tag="J1Y83_000258"
     CDS             complement(18199..18975)
                     /gene="sseB"
                     /locus_tag="J1Y83_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311415.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enhanced serine sensitivity protein SseB"
                     /protein_id="HBA7628447.1"
                     /translation="MSETKNELEDLLEKAATEPAHRPAFFRTLLESTVWVPGTAAQGE
                     AVVEDSALDLQHWEKEDGTSVIPFFTSLEALQQAVEDEQAFVVMPVRTLFEMTLGETL
                     FLNAKLPTGKEFMPREISLLIGEEGNPLSSQEVLEGGESLILSEVAEPPVQMIDSLTT
                     LFKTIKPVKRAFICLIKESEEAQPNLLIGIEADGDIEEIIQAAGSVATDTLPGDEPID
                     ICQVKKGEKGISHFITEHIAPFYERRWGGFLRDFKQNRII"
     gene            complement(19117..20400)
                     /gene="pepB"
                     /locus_tag="J1Y83_000259"
     CDS             complement(19117..20400)
                     /gene="pepB"
                     /locus_tag="J1Y83_000259"
                     /EC_number="3.4.11.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311416.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase PepB"
                     /protein_id="HBA7628448.1"
                     /translation="MTEAMKITLSTQPADARWGEKATYSINNDGITLHLNGADDLGLI
                     QRAARKIDGLGIKHVQLSGEGWDADRCWAFWQGYKAPKGTRKVEWPDLDDVQRQELDN
                     RLMIIDWVRDTINAPAEELGPSQLAQRAVDLISNVAGDRVTYRITKGEDLRDQGYMGL
                     HTVGRGSERSPVLLALDYNPTGDKEAPVYACLVGKGITFDSGGYSIKQTAFMDSMKSD
                     MGGAATVTGALAFAITRGLNKRVKLFLCCADNLISGNAFKLGDIITYRNGKKVEVMNT
                     DAEGRLVLADGLIDASAQKPELIIDAATLTGAAKTALGNDYHALFSFDDALAGRLLAS
                     ASQENEPFWRLPLAEFHRSQLPSNFAELNNTGSAAYPAGASTAAGFLSHFVENYQQGW
                     LHIDCSATYRKAPVEQWSAGATGLGVRTIANLLTA"
     gene            complement(20578..20778)
                     /gene="iscX"
                     /locus_tag="J1Y83_000260"
     CDS             complement(20578..20778)
                     /gene="iscX"
                     /locus_tag="J1Y83_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004104502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein IscX"
                     /protein_id="HBA7628449.1"
                     /translation="MGLKWTDSREIGEALYDAYPDIDPKTVRFTDMHQWICDLEDFDD
                     DPQASNEKILEAILLVWLDEAE"
     gene            complement(20790..21125)
                     /gene="fdx"
                     /locus_tag="J1Y83_000261"
     CDS             complement(20790..21125)
                     /gene="fdx"
                     /locus_tag="J1Y83_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015683702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISC system 2Fe-2S type ferredoxin"
                     /protein_id="HBA7628450.1"
                     /translation="MPKIVILPHQDLCPDGAVLEANSGETILDVALRNGIEIEHACEK
                     SCACTTCHCIVREGFDSLPESSEQEDDMLDKAWGLEPESRLSCQARVTDEDLVVEIPR
                     YTINHAREH"
     gene            complement(21127..22977)
                     /gene="hscA"
                     /locus_tag="J1Y83_000262"
     CDS             complement(21127..22977)
                     /gene="hscA"
                     /locus_tag="J1Y83_000262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001559634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S protein assembly chaperone HscA"
                     /protein_id="HBA7628451.1"
                     /translation="MALLQISEPGLSAAPHQRRLAAGIDLGTTNSLVATVRSGQAETL
                     ADHEGCHLLPSVVHYQQQGHSVGYDARTNAALDTANTISSVKRLMGRSLADIQQRYPH
                     LPYQFQSSENGLPMIETAAGLLNPVRVSADILKALAARATEALAGELDGVVITVPAYF
                     DDAQRQGTKDAARLAGLHVLRLLNEPTAAAIAYGLDSGQEGVIAVYDLGGGTFDISIL
                     RLSRGVFEVLATGGDSALGGDDFDHLLADYIREQAGIPDRSDNRVQRELLDAAIAAKI
                     ALSDADSVTVNVAGWQGEISREQFNELIAPLVKRTLLACRRALKDAGVEADEVLEVVM
                     VGGSTRVPLVRERVGEFFGRPPLTSIDPDKVVAIGAAIQADILVGNKPDSEMLLLDVI
                     PLSLGLETMGGLVEKVIPRNTTIPVARAQDFTTFKDGQTAMSIHVMQGERELVQDCRS
                     LARFALRGIPALPAGGAHIRVTFQVDADGLLSVTAMEKSTGVEASIQVKPSYGLTDSE
                     IASMIKDSMSYAEQDVKARMLAEQKVEAARVLESLHGALAADAALLSAAERQAIDDAA
                     AHLSEVAQGDDVDAIEQAIKNVDKQTQDFAARRMDQSVRRALKGHSVDEV"
     gene            complement(22994..23509)
                     /gene="hscB"
                     /locus_tag="J1Y83_000263"
     CDS             complement(22994..23509)
                     /gene="hscB"
                     /locus_tag="J1Y83_000263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012131249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone HscB"
                     /protein_id="HBA7628452.1"
                     /translation="MDYFTLFGLPARYQLDTQALSLRFQDLQRQYHPDKFASGSQAEQ
                     LAAVQQSATINQAWQTLRHPLMRAEYLLSLHGFDLASEQHTVRDTAFLMEQLELREEL
                     DEIEQAKDEARLESFIKRVKKMFDTRHQLMVEQLDNETWDAAADTVRKLRFLDKLRSS
                     AEQLEEKLLDF"
     gene            complement(23605..23928)
                     /gene="iscA"
                     /locus_tag="J1Y83_000264"
     CDS             complement(23605..23928)
                     /gene="iscA"
                     /locus_tag="J1Y83_000264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017373466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly protein IscA"
                     /protein_id="HBA7628453.1"
                     /translation="MSITLSDSAAARVNTFLANRGKGFGLRLGVRTSGCSGMAYVLEF
                     VDEPTPEDIVFEDKGVKVVVDGKSLQFLDGTQLDFVKEGLNEGFKFTNPNVKDECGCG
                     ESFHV"
     gene            complement(23945..24331)
                     /gene="iscU"
                     /locus_tag="J1Y83_000265"
     CDS             complement(23945..24331)
                     /gene="iscU"
                     /locus_tag="J1Y83_000265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold IscU"
                     /protein_id="HBA7628454.1"
                     /translation="MAYSEKVIDHYENPRNVGSFDNNDENVGSGMVGAPACGDVMKLQ
                     IKVNDEGIIEDARFKTYGCGSAIASSSLVTEWVKGKSLDEAQAIKNTDIAEELELPPV
                     KIHCSILAEDAIKAAIADYKSKREAK"
     gene            complement(24359..25573)
                     /gene="iscS"
                     /locus_tag="J1Y83_000266"
     CDS             complement(24359..25573)
                     /gene="iscS"
                     /locus_tag="J1Y83_000266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311423.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HBA7628455.1"
                     /translation="MKLPIYLDYSATTPVDPRVAEKMMQFMTMDGTFGNPASRSHRFG
                     WQAEEAVDIARNQIADLVGADPREIVFTSGATESDNLAIKGAANFYQKKGKHIITSKT
                     EHKAVLDTCRQLEREGFEVTYLAPQRNGIIDLKELEAAMRDDTILVSIMHVNNEIGVV
                     QDIAAIGEMCRARGIIYHVDATQSVGKLPIDLSQLKVDLMSFSGHKIYGPKGIGALYV
                     RRKPRVRIEAQMHGGGHERGMRSGTLPVHQIVGMGEAYRIAKEEMATEMERLRGLRNR
                     LWNGIKDIEEVYLNGDLEHGAPNILNVSFNYVEGESLIMALKDLAVSSGSACTSASLE
                     PSYVLRALGLNDELAHSSIRFSLGRFTTEEEIDYTIELVRKSIGRLRDLSPLWEMYKQ
                     GVDLNSIEWAHH"
     gene            complement(25685..26173)
                     /gene="iscR"
                     /locus_tag="J1Y83_000267"
     CDS             complement(25685..26173)
                     /gene="iscR"
                     /locus_tag="J1Y83_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002913993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly transcriptional regulator
                     IscR"
                     /protein_id="HBA7628456.1"
                     /translation="MRLTSKGRYAVTAMLDVALNSEAGPVPLADISERQGISLSYLEQ
                     LFSRLRKNGLVSSVRGPGGGYLLGKDASSIAVGEVISAVDESVDATRCQGKGGCQGGD
                     KCLTHALWRDLSDRLTGFLNNITLGELVNNQEVLDVSGRQHTHDAPRTRTQDAIDVKL
                     RA"
     gene            complement(26443..27183)
                     /gene="trmJ"
                     /locus_tag="J1Y83_000268"
     CDS             complement(26443..27183)
                     /gene="trmJ"
                     /locus_tag="J1Y83_000268"
                     /EC_number="2.1.1.200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (cytosine(32)/uridine(32)-2'-O)-methyltransferase TrmJ"
                     /protein_id="HBA7628457.1"
                     /translation="MLQNIRIVLVETSHTGNMGSVARAMKTMGLTNLWLVNPLVKPDS
                     QAIALAAGASDVIGNAHIVDTLDEALAGCSLVVGTSARSRTLPWPMLDPRECGLKSVA
                     EAANTPVALVFGRERVGLTNEELQKCHYHVAIAANPEYSSLNLAMAVQVIAYEVRMAW
                     LATQENGEQVEHEETPYPLVDDLERFYGHLEQTLLATGFIRENHPGQVMNKLRRLFTR
                     ARPESQELNILRGILASIEQQNKGNKAE"
     gene            27302..28105
                     /gene="suhB"
                     /locus_tag="J1Y83_000269"
     CDS             27302..28105
                     /gene="suhB"
                     /locus_tag="J1Y83_000269"
                     /EC_number="3.1.3.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000553460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol-1-monophosphatase"
                     /protein_id="HBA7628458.1"
                     /translation="MHPMLNIAVRAARKAGNLIAKNYETPDAVEASQKGSNDFVTNVD
                     KAAEAVIIDTIRKSYPQHTIITEESGELEGTDQDVQWVIDPLDGTTNFIKRLPHFAVS
                     IAVRIKGRTEVAVVYDPMRNELFTATRGQGAQLNGYRLRGSTARDLDGTILATGFPFK
                     AKQYATTYINIVGKLFNECADFRRTGSAALDLAYVAAGRVDGFFEIGLRPWDFAAGEL
                     LVREAGGIVSDFTGGHNYMLTGNIVAGNPRVVKAMLANMRDELSDALKR"
     gene            28250..29104
                     /locus_tag="J1Y83_000270"
     CDS             28250..29104
                     /locus_tag="J1Y83_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417029.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBA7628459.1"
                     /translation="MALPVNKRVLKILFILFVVAFCVYLVPRVAINFFYYPDDKIYGP
                     DPWSAESVEFTAKDGTRLQGWFIPSSTGPADNAIATIIHAHGNAGNMSAHWPLVSWLP
                     ERNFNVFMFDYRGFGKSKGTPSQAGLLDDTQSAINVVRHRSDVNPQRLVLFGQSIGGA
                     NILDVIGQGDREGIRAVILDSTFASYATIANQMIPGSGYLLDESYSGENYIASVSPIP
                     LLLIHGKADHVIPWQHSEKLYSLAKEPKRLILIPDGEHIDAFSDRHGDVYREQMVDFI
                     LSALNPQN"
     gene            29295..30575
                     /gene="csiE"
                     /locus_tag="J1Y83_000271"
     CDS             29295..30575
                     /gene="csiE"
                     /locus_tag="J1Y83_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stationary phase inducible protein CsiE"
                     /protein_id="HBA7628460.1"
                     /translation="MMPTLAPPSVLSAPQRRCQILLTLFQPGLTATTATFSELNGVDD
                     DIASLDISETGREILRYHQLTLTTGYDGSYRVEGTVLNQRLCLFHWLRRGFRLCPSFI
                     TSHFTPALKSELKRRGIARNFYDDTNLQALVNLCSRRLQKRFETRDIHFLCLYLQYCL
                     LQHHAGITPQFNPLQRRWAESCLEFQVAQEIGRHWQRRALQPVPPDEPLFMALLFSML
                     RVPDPLRDAHQRDRQLRQSIKRLVNHFRELGNVRFYDEQGLCDQLYTHLAQALNRSLF
                     AIGIDNTLPEEFARLYPRLVRTTRAALTGFESEYGVHLSDEESGLVAVIFGAWLMQEN
                     DLHEKQIILLTGNDSEREAQIEQQLRELTLLPLNIKHMSVKAFLQTGAPRGAALIIAP
                     YTMPLPLFSPPLIYTDLTLTTHQQEQIRKMLESA"
     gene            complement(30567..31706)
                     /locus_tag="J1Y83_000272"
     CDS             complement(30567..31706)
                     /locus_tag="J1Y83_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate MFS transporter"
                     /protein_id="HBA7628461.1"
                     /translation="MVLQSTRWLALGYFTYFFSYGIFLPFWSVWLKGIGLTPETIGLL
                     LGAGLVARFLGSLLIAPRVSDPSRLISALRVLALLTLLFAVAFWAGAHVAWLMLVMIG
                     FNLFFSPLVPLTDALANTWQKQFPLDYGKVRLWGSVAFVIGSALTGKLVSMFDYRVIL
                     ALLTLGVASMLLGFLIRPTIQPQGASRQQESTGWSAWLALVRQNWRFLACVCLLQGAH
                     AAYYGFSAIYWQAAGYSASAVGYLWSLGVVAEVIIFALSNKLFRRCSARDMLLISAIC
                     GVVRWGIMGATTALPWLIVVQILHCGTFTVCHLAAMRYIAARQGSEVIRLQAVYSAVA
                     MGGSIAIMTVFAGFLYQYLGHGVFWVMALVALPAMFLRPKVVPSC"
     gene            32075..32497
                     /locus_tag="J1Y83_000273"
     CDS             32075..32497
                     /locus_tag="J1Y83_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311436.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HBA7628462.1"
                     /translation="MNSLRYFDFGTARPVLLLIARIAVVLIFIIFGFPKMMGFDGTVQ
                     YMASLGAPMPMLAAIIAVVMEVPAAILIILGFFTRPLAVLFIFYTLGTAVIGHHYWDM
                     TGDAVGPNMINFWKNISIAGAFLLLAITGPGAISLDRR"
     gene            complement(32657..33529)
                     /locus_tag="J1Y83_000274"
     CDS             complement(32657..33529)
                     /locus_tag="J1Y83_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase"
                     /protein_id="HBA7628463.1"
                     /translation="MTIYTLSHGPLKLDVSDQGGVIEGFWRDTTPLLRPGKKSGVATD
                     ASCFPLVPFANRVSGNRFVWQGREYQLQPNVEWDAHYLHGDGWLGEWQCVSRSDDSLC
                     LVYEHLSGVYHYRVSQAFHLTADTLTVTLAVTNQGAETLPFGTGWHPYFPLSPQTRIQ
                     AQASGYWLEREQWLAGEFCEQLPQELDFNQLAPLPRQWVNNGFAGWNGKARIEQPQEG
                     YAIIMETTPPAQCYFIFVSDPAFDKGYAFDFFCLEPMSHAPDDHHRPEGGDLIALAPG
                     ESTISEMSLRVALL"
     gene            complement(33540..34634)
                     /locus_tag="J1Y83_000275"
     CDS             complement(33540..34634)
                     /locus_tag="J1Y83_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HBA7628464.1"
                     /translation="MKINRIVNEGFMKTMLAAYLPGNSTVDLREVAVPTPGINQVLIK
                     MKSSGICGSDVHYIYHQHRATAAAPDKPLYQGFINGHEPCGQIVAMGQGCRHFKEGDR
                     VLVYHISGCGFCPNCRRGFPISCTGEGKAAYGWQRDGGHAEYLLAEEKDLILLPDALS
                     YEDGAFISCGVGTAYEGILRGEVSGSDNVLVVGLGPVGMMAMMLAKGRGAKRIIGVDM
                     LPERLAMAKQLGVMDHGYLATTEGLPQIIAELTHGGADVALDCSGNAAGRLLALQSTA
                     DWGRVVYIGETGKVEFEVSADLMHHQRRIIGSWVTSLFHMEKCAHDLTDWKLWPRNAI
                     THRFSLEQAGDAYALMASGKCGKVVINFPD"
     gene            complement(34667..35665)
                     /locus_tag="J1Y83_000276"
     CDS             complement(34667..35665)
                     /locus_tag="J1Y83_000276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBA7628465.1"
                     /translation="MSASSLPLPQGKSVSLKQFVSRHINEIGLLVVIAILYLVFSLNA
                     PGFISLNNQMNVLRDAATIGIAAWAMTLIIISGEIDVSVGPMVAFVSVCLAFLLQFDV
                     PLAIACLLVLLLGALMGTLAGVLRGVFNVPSFVATLGLWSALRGMGLFMTNALPVPIN
                     ENEVLDWLGGQFLGVPVSALIMMVLFALFVFISRKTAFGRSVFAVGGNATAAQLCGIN
                     VRRVRILIFTLSGLLAAVTGILLAARLGSGNAGAANGLEFDVIAAVVVGGTALSGGRG
                     SLFGTLLGVLVITLIGNGLVLLGINSFFQQVVRGVIIVVAVLANTLLTQRSSKAKR"
     gene            complement(35690..37201)
                     /locus_tag="J1Y83_000277"
     CDS             complement(35690..37201)
                     /locus_tag="J1Y83_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HBA7628466.1"
                     /translation="MFTATEAVPVAKVVAGNKRYPGVVALDNVNFTLNKGEVRALLGK
                     NGAGKSTLIRMLTGSERPDSGDIWIGETRLEGDEATLTRRAAELGVRAVYQELSLVEG
                     LTVAENLCLGQWPRRNGMIDYLQMAQDAQRCLQALGVDVSPEQLVSTLSPAQKQLVEI
                     ARVMKGEPRVVILDEPTSSLASAEVELVISAVKKMSALGVAVIYVSHRMEEIRRIASC
                     ATVMRDGQVAGDVMLENTSTHHIVSLMLGRDHVDIAPVAPQEIMDQAVLEVRALRHKP
                     KLEDISFTLRRGEVLGIAGLLGAGRSELLKAIVGLETYEQGEIVINGEKITRPDYGDM
                     LKRGIGYTPENRKEAGIIPWLGVDENTVLTNRQKISANGVLQWSTIRRLTEEVMQRMT
                     VKAASSETPIGTLSGGNQQKVVIGRWVYAASQILLLDEPTRGVDIEAKQQIYRIVREL
                     AAEGKSVVFISSEVEELPLVCDRILLLQHGTFSQEFHSPVNVDELMSAILSVH"
     gene            complement(37224..38207)
                     /locus_tag="J1Y83_000278"
     CDS             complement(37224..38207)
                     /locus_tag="J1Y83_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HBA7628467.1"
                     /translation="MPKKMTRTRNLLLMATLLGSALFARASDKEMTIGAIYLDTQGYY
                     AGVRQGVQDAAKDSSVQVQLIETNAQGDISKESTFVDTLVARNVDAIILSAVSENGSS
                     RTVRRASEAGIPVICYNTCINQKGVDKYVSAYLVGDPLEFGKKLGNAAADYFIANKID
                     QPKIAVINCEAFEVCVQRRKGFEEVLKTRVPGAQIVANQEGTVLDKAISVGEKLIIST
                     PDLNAIMGESGGATLGAVKAVRNQNQAGKIAVFGSDMTTEIAQELENNQVLKAVVDIS
                     GKKMGNAVFAQTLKVINKQADGEKVIQVPIDLYTKTEDGKQWLATHVDGLP"
     gene            complement(38304..41585)
                     /locus_tag="J1Y83_000279"
     CDS             complement(38304..41585)
                     /locus_tag="J1Y83_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5107 domain-containing protein"
                     /protein_id="HBA7628468.1"
                     /translation="MTPVKVWQERVEIPTYETGPQDIHPMFLENRVYQGSSGAVYPYG
                     VTDTLSEQKTLKSWQAVWLENDYIKVMILPELGGRVHRAWDKVKQRDFVYHNEVIKPA
                     LVGLLGPWISGGIEFNWPQHHRPTTFMPVDFTLEAHDDGAQTVWVGETEPMHGLQVMT
                     GFTLRPDRAALEIASRVYNGNATPRHFLWWANPAVKGGEGHQSVFPPDVTAVFDHGKR
                     AVSAFPIATGTYYKVDYSAGVDISRYKNVPVPTSYMAEKSQYDFVGAWCHDEDGGLLH
                     VANHHIAPGKKQWSWGHSEFGQAWDKSLTDNNGPYIELMTGIFADNQPDFTWLDAYEE
                     KRFEQYFLPYHSLGMVQNASRDAVIKLQRSERGIEWGLYAISPLNGYRLAIREIGKCN
                     ALLDDAVALTPATAIQGVLHGINPERLTIELSDADGNIVLSYHEHQPQALPLPDVAKA
                     PLAAQDITSTDEAWFIGQHLEQYHHASRSPFDYYLRGVALDPLDYRCNLALAMLEYNR
                     ADFPQAVAYATQALKRAHALNKNPQCGQASLIRASAYERQGQYQQAEEDFWRAVWSGN
                     SKAGGYYGLARLAARNGHFDAGLDFCQQSLRACPTNQEVLCLHNLLLVLSGRQDNARL
                     QREKLLRDYPLNATLWWLNWFDGRSESALAQWRGLCQGRDVNALITAGQLINWGMPAL
                     AADMLNALDCQRTLPLYLQASLLPKAERGELVVKAIDAFPQFVRFPNTLEEVAALESI
                     EECWFARHLLACFYYNKRSYGKAIALWQRCVEMSPEFADGWRGLAIHAWNKQHDYELA
                     ARYLDNAYQLAPQDARLLFERDLLDKLSGVTPEKRLARLENNLEIALKRDDMTAELLN
                     LWHLTGQADKAADILATRKFHPWEGGEGKVTSQFILNQLLRAWQHLDDREPQQASELL
                     HAALHYPENLSEGRLPGQTDNDIWFWQAVCANAQGDETEATRCLRLAATGDRTINIHS
                     YYNDQPVDYLFWQGMALRLLGEQHTAQQLFSEMKQWAKEMAKTSIEADFFAVSQPDLL
                     SLYGDLQQQHKEKCLMVAMLAAAGLGEVAHYESARAELMAINPAWPKAALFTTVMPFI
                     FSYVH"
     gene            41703..42896
                     /locus_tag="J1Y83_000280"
     CDS             41703..42896
                     /locus_tag="J1Y83_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311443.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HBA7628469.1"
                     /translation="MRACINNQQIRHHNKCVILELLYRQKRANKSTLARLAQISIPAV
                     SNILQELESEKRVVNIDDESQTRGHSSGTWLIAPEGDWTLCLNVTPTSIECQVANACL
                     SPKGEFEYLQIDAPTPQALLSEIEKCWHRHRKLWPDRTINLALAIHGQVDPVTGVSQT
                     MPQAPWATPIEVKYLLEEKLGIRVMVDNDCVMLALAEKWQNNSQERDFCVINVDYGIG
                     SSFVINEQIYRGSLYGSGQIGHTIVNPDGVVCDCGRYGCLETVASLSALKKQARVWLK
                     SQPVNTQLDPEKLTTAQLIAAWQSGEPWITSWVDHSANAIGLSLYNFLNILNINQIWL
                     YGRSCAFGENWLNTIIRQTGFNPFDRDEGPSVKATQIGFGQLSRAQQVLGIGYLYVEA
                     QLRQI"
     gene            complement(43094..44347)
                     /locus_tag="J1Y83_000281"
     CDS             complement(43094..44347)
                     /locus_tag="J1Y83_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019077397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HBA7628470.1"
                     /translation="MLKREMNIADYDAELWQAMEQEKVRQEEHIELIASENYTSPRVM
                     QAQGSQLTNKYAEGYPGKRYYGGCEYVDIVEQLAIDRAKELFGADYANVQPHSGSQAN
                     FAVYTALLEPGDTVLGMNLAHGGHLTHGSPVNFSGKLYNIVPYGIDATGHIDYADLEK
                     QAKEHKPKMIIGGFSAYSGVVDWAKMREIADSIGAYLFVDMAHVAGLVAAGVYPNPVP
                     HAHVVTTTTHKTLAGPRGGLILAKGGSEELYKKLNSAVFPGGQGGPLMHVIAGKAVAL
                     KEAMEPEFKTYQQQVAKNAKAMVEVFLERGYKVVSGGTDNHLFLVDLVDKNLTGKEAD
                     AALGRANITVNKNSVPNDPKSPFVTSGIRVGTPAITRRGFKEAEAKELAGWMCDVLDS
                     INDEAVIERIKGKVLDICARYPVYA"
     gene            44676..45866
                     /gene="hmpA"
                     /locus_tag="J1Y83_000282"
     CDS             44676..45866
                     /gene="hmpA"
                     /locus_tag="J1Y83_000282"
                     /EC_number="1.14.12.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NO-inducible flavohemoprotein"
                     /protein_id="HBA7628471.1"
                     /translation="MLDAQTIATVKATIPLLVETGPKLTAHFYDRMFTHNPELKEIFN
                     MSNQRNGDQREALFNAIAAYASNIENLPALLPAVEKIAQKHTSFQIKPEQYNIVGEHL
                     LATLDEMFSPGQEVLDAWGKAYGVLANVFINREAEIYNENASKAGGWEGTRDFRIVAK
                     TPRSALITSFELEPVDGGAVAEYRPGQYLGVWLKPEGFPHQEIRQYSLTRKPDGKGYR
                     IAVKREEGGQVSNWLHNHANVGDVVKLVAPAGDFFMAVADDTPVTLISAGVGQTPMLA
                     MLDTLAKAGHTAQVNWFHAAENGDVHAFADEVKELGQSLPRFTAHTWYRQPNEADRAK
                     GQFDSEGLMDLSKLEGAFSDPTMQFYLCGPVGFMQFAAKQLVDLGVKQENIHYECFGP
                     HKVL"
     gene            complement(45911..46249)
                     /gene="glnB"
                     /locus_tag="J1Y83_000283"
     CDS             complement(45911..46249)
                     /gene="glnB"
                     /locus_tag="J1Y83_000283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017373484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulatory protein P-II"
                     /protein_id="HBA7628472.1"
                     /translation="MKKIDAIIKPFKLDDVREALAEVGITGMTVTEVKGFGRQKGHTE
                     LYRGAEYMVDFLPKVKIEIVVPDDIVDTCVDTIIRTAQTGKIGDGKIFVFDVARVIRI
                     RTGEEDDAAI"
     gene            complement(46310..47644)
                     /gene="glrR"
                     /locus_tag="J1Y83_000284"
     CDS             complement(46310..47644)
                     /gene="glrR"
                     /locus_tag="J1Y83_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator GlrR"
                     /protein_id="HBA7628473.1"
                     /translation="MSHKPAHLLLVDDDPGLLKLLGLRLTSEGYSVVTAESGAEGLRV
                     LNREKVDLVISDLRMDEMDGMQLFAEIQKVQPGMPVIILTAHGSIPDAVAATQQGVFS
                     FLTKPVDKDALYQAIDDALEQSAPATDERWREAIVTRSPLMLRLLEQARLVAQSDVSV
                     LINGQSGTGKEIFAQAIHNASPRNSKPFIAINCGALPEQLLESELFGHARGAFTGAVS
                     NREGLFQAAEGGTLFLDEIGDMPAPLQVKLLRVLQERKVRPLGSNRDIDIDVRIISAT
                     HRDLPKAMARGEFREDLYYRLNVVSLKIPALAERTEDIPLLANHLLRQAAERHKPFVR
                     AFSTDAMKRLMTASWPGNVRQLVNVIEQCVALTSSPVISDALVEQALEGENTALPTFV
                     EARNQFELNYLRKLLQITKGNVTHAARMAGRNRTEFYKLLSRHELDANDFKE"
     gene            complement(47634..48347)
                     /gene="qseG"
                     /locus_tag="J1Y83_000285"
     CDS             complement(47634..48347)
                     /gene="qseG"
                     /locus_tag="J1Y83_000285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system QseEF-associated
                     lipoprotein QseG"
                     /protein_id="HBA7628474.1"
                     /translation="MRHIFQRLLPRRLWLAGLPCLALLGCVQSHNRPAIDTPAEEKIP
                     VYQLADYLSTECSDIWALQGKSTETNPLYWLRAMDCADRLMPAQSRQQARQYDDGNWQ
                     NTFKQGILLADAKITPYERRQLVARIDALSTEIPAQVRPLYQLWRDGQALQLQLAEER
                     QRYSKLQQSSDSELDTLRQQHHVLQQQLELTTRKLENLTDIERQLSTRKPAGNFSPDT
                     PHESEKPAPSTDEVTPDEP"
     gene            complement(48512..49939)
                     /gene="qseE"
                     /locus_tag="J1Y83_000286"
     CDS             complement(48512..49939)
                     /gene="qseE"
                     /locus_tag="J1Y83_000286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417051.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two component system sensor histidine kinase
                     QseE/GlrK"
                     /protein_id="HBA7628475.1"
                     /translation="MKRWPVFPRSLRQLVMLAFLLILLPLLVLAWQAWQSLNALSDQA
                     ALVNRTTLIDARRSEAMTNAALEMERSYRQYCVLDDPTLAKVYQSQRKRYSEMLDAHA
                     GVLPDDKLYQALRQDLNNLAQLQCNNSGPDAAAAARLEAFASANTEMVQATRTVVFSR
                     GQQLQREIAERGQYFGWQSLVLFLVSLVMVLLFTRMIIGPVKNIERMINRLGEGRSLG
                     NSVSFSGPSELRSVGQRILWLSERLSWLESQRHQFLRHLSHELKTPLASMREGTELLA
                     DQVVGPLTPEQKEVVSILDSSSRNLQKLIEQLLDYNRKQADSAVELENVELAPLVETV
                     VSAHSLPARAKMMHTDVDLKATACLAEPMLLMSVLDNLYSNAVHYGAESGNICLRSSL
                     HGARVYIDVINTGTPIPQEERAMIFEPFFQGSHQRKGAVKGSGLGLSIARDCIRRMQG
                     ELYLVDESGQDVCFRIELPSSKNTK"
     gene            complement(50515..54402)
                     /gene="purL"
                     /locus_tag="J1Y83_000287"
                     /gene_synonym="purI"
     CDS             complement(50515..54402)
                     /gene="purL"
                     /locus_tag="J1Y83_000287"
                     /gene_synonym="purI"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006683423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HBA7628476.1"
                     /translation="MMEILRGSPALSAFRINKLLARFQAARLPVHTIYAEYVHFADLN
                     APLNDDEHAQLERLLKYGPALASHAPQGKLLLVTPRPGTISPWSSKATDIAHNCGLQQ
                     VNRLERGVAYYIEAGTLTNEQWQQVTAELHDRMMETVFFALDDAEQLFAHHQPTPVTS
                     VDLLGQGRQALIDANLRLGLALAEDEIDYLQDAFTKLGRNPNDIELYMFAQANSEHCR
                     HKIFNADWVIDGEQQPKSLFKMIKNTFETTPDHVLSAYKDNAAVMEGSEVGRYFADHE
                     TGRYDFHQEPAHILMKVETHNHPTAISPWPGAATGSGGEIRDEGATGRGAKPKAGLVG
                     FSVSNLRIPGFEQPWEEDFGKPERIVTALDIMTEGPLGGAAFNNEFGRPALNGYFRTY
                     EEKVNSHNGEELRGYHKPIMLAGGIGNIRADHVQKGEINVGAKLVVLGGPAMNIGLGG
                     GAASSMASGQSDADLDFASVQRDNPEMERRCQEVIDRCWQLGDANPILFIHDVGAGGL
                     SNAMPELVSDGGRGGKFELRDILSDEPGMSPLEIWCNESQERYVLAVAADQLPLFDEL
                     CKRERAPYAVIGEATEELHLSLHDRHFDNQPIDLPLDVLLGKTPKMTRDVQTLKAKGD
                     ALAREGITIADAVKRVLHLPTVAEKTFLVTIGDRSVTGMVARDQMVGPWQVPVANCAV
                     TTASLDSYYGEAMAIGERAPVALLDFAASARLAVGEALTNIAATQIGDIKRIKLSANW
                     MAAAGHPGEDAGLYEAVKAVGEELCPALGLTIPVGKDSMSMKTRWQEGSEEREMTSPL
                     SLVISAFARVEDVRHTITPQLSTEDNALLLIDLGKGNNALGATALAQVYRQLGDKPAD
                     VRDVAQLKGFYDAIQALVAQRKLLAYHDRSDGGLLVTLAEMAFAGHCGIDADIATLGD
                     DRLAALFSEELGAVIQVRAADREAVEAVLAQHGLADCVHYVGQAVPGDRFVITANGQT
                     VFSESRTTLRVWWAETTWQMQRLRDNPECADQEHQAKSNDADPGLNVKLSFDINEDVA
                     APYIATGARPKVAVLREQGVNSHVEMAAAFHRAGFDAIDVHMSDLLAGRTGLEDFHAL
                     VACGGFSYGDVLGAGEGWAKSILFNDRVRDEFATFFHRPQTLALGVCNGCQMMSNLRE
                     LIPGSELWPRFVRNTSDRFEARFSLVEVTQSPSLLLQGMVGSQMPIAVSHGEGRVEVR
                     DAAHLAALESKGLVALRYVDNFGKVTETYPANPNGSPNGITAVTTESGRVTIMMPHPE
                     RVFRTVSNSWHPENWGEDGPWMRIFRNARKQLG"
     gene            54660..56216
                     /gene="mltF"
                     /locus_tag="J1Y83_000288"
     CDS             54660..56216
                     /gene="mltF"
                     /locus_tag="J1Y83_000288"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417053.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     MltF"
                     /protein_id="HBA7628477.1"
                     /translation="MKKLKINYLFIGILALLLAVALWPSIPWFGKADNRIAAIQARGE
                     LRVSTIHTPLTYNEINGKPFGLDYELAKQFADYLGVKLKVTVRQNISQLFDDLDNGNA
                     DLLAAGLVYNSERVKNYQPGPTYYSVSQQLVYKVGQYRPRTLGNLTAEQLTVAPGHVV
                     VNDLQTLKDTKFPELSWKVDDKKGSVELMEDVIEGKLDYTIADSVAISLFQRVHPELA
                     VALDITDEQPVTWFSPLDGDNTLSAALLDFFNEMNEDGTLARIEEKYLGHGDDFDYVD
                     TRTFLRAVDAVLPQLKPLFEKYAEEIDWRLLAAIAYQESHWDAQATSPTGVRGMMMLT
                     KNTAQSLGITDRTDAEQSISGGVRYLQDMMSKVPESVPENERIWFALAAYNMGYAHML
                     DARALTAKTKGNPDSWADVKQRLPLLSQKPYYSKLTYGYARGHEAYAYVENIRKYQIS
                     LVGYLQEKEKQATEAAMQLAQDYPAVSPTELGKEKFPFLSFLSQSSSNYLTHSPSLLF
                     SRKGSEEKQN"
     gene            complement(56213..56749)
                     /gene="tadA"
                     /locus_tag="J1Y83_000289"
     CDS             complement(56213..56749)
                     /gene="tadA"
                     /locus_tag="J1Y83_000289"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HBA7628478.1"
                     /translation="MRRAFITGVFFLSEVEFSHEYWMRHAMTLAKRAWDEREVPVGAV
                     LVHNNRVIGEGWNRPIGRHDPTAHAEIMALRQGGLVMQNYRLIDATLYVTLEPCVMCA
                     GAMIHSRIGRVVFGARDAKTGAAGSLMDVLHHPGMNHRVEITEGILADECAALLSDFF
                     RMRRQEIKAQKKAQSSTD"
     gene            complement(56774..57409)
                     /gene="yfhb"
                     /locus_tag="J1Y83_000290"
     CDS             complement(56774..57409)
                     /gene="yfhb"
                     /locus_tag="J1Y83_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311453.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase C"
                     /protein_id="HBA7628479.1"
                     /translation="MATHERRVVFFDLDGTLHQQDMFGSFLRYLLRRQPLNALLVLPL
                     LPIIAIALLIKGRAARWPMSLLLWGCTFGHNEARLQALQADFVRWFRDNVTAFPLVQE
                     RLTTYLLSSDADIWLITGSPQPLVEAVYFDTPWLPRVNLIASQIQRGYGGWVLTMRCL
                     GHEKVAQLERKIGTPLRLYSGYSDSNQDNPLLYFCQHRWRVTPRGELQQLE"
     gene            57618..58466
                     /locus_tag="J1Y83_000291"
     CDS             57618..58466
                     /locus_tag="J1Y83_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HBA7628480.1"
                     /translation="MNGLLRIRQRYQGLAQSDKKLADYLLLQPDTARHLSSQQLANEA
                     GVSQSSVVKFAQKLGYKGFPALKLALSEALASQPESPSVPIHNQIRGDDPLRLVGEKL
                     IKENTAAMYATLNVNSEEKLHECVAMLRSARRIILTGIGASGLVAQNFAWKLMKIGFN
                     AAAVRDMHALLATVQASSPDDLLLAISYTGVRRELNLAADEMLRVGGKVLAITGFTPN
                     ALQQRASHCLYTIAEEQATNSASISACHAQGMLTDLLFIALIQQDLELAPERIRHSEA
                     LVKKLV"
     gene            58522..58782
                     /gene="yfhL"
                     /locus_tag="J1Y83_000292"
     CDS             58522..58782
                     /gene="yfhL"
                     /locus_tag="J1Y83_000292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster ferredoxin YfhL"
                     /protein_id="HBA7628481.1"
                     /translation="MALLITKKCINCDMCEPECPNEAISMGEHIYEINSDKCTECVGH
                     YETPTCQKVCPIPNTIVKDPQHVETEEQLWDKFVLMHHADKI"
BASE COUNT        13932 a        16384 c        14680 g        13851 t
ORIGIN      
        1 cgttttttag atattaaacc gtgcccatgt tggcagaaat aatccacagc accacaagct
       61 tttaacactt gtaacgcctg atgacactca gggcaaagtg cctggacggt aaagtctttg
      121 tcgcaagttg cacagtgagc agtctcgcca ttgcgttcga gaggggtttt gcaggttgga
      181 cagttgagtg ccataaatac tcctcgcgtg ttattcggct tccctctccg tgtggaaagg
      241 gaaaccagat aagtgattac ttacttttct tgatatgctt catcagacgc ttacgtttac
      301 gcatctgggt tggcgtcagg gtgttacgct tgttcgcata cgggttttcc ccttctttga
      361 actgaatacg aatcggcgat cccattacgt ccagcgattt gcggaagtag ttcatcaggt
      421 aacgcttgta ggaatcaggc aggtctttca cctgattgcc gtgaatcacc acaatcggcg
      481 ggttataacc cccggcgtgg gcgtatttca gcttcacacg acgaccgcgt accagcggtg
      541 gttggtgatc ttcaacagcc atcgtcatga tgcgcgtcag catagaggtc cccacacgac
      601 gggtggagct gtcatacgct tcacgtactg attcaaacaa gttaccaaca ccactgccgt
      661 gcaaggcaga gataaagtgc acacgagcaa aatcgataaa gcccagacgg aagtccagcg
      721 tctctttcac ctgctctttc acttcctggc tcaggccgtc ccacttgttc actacaatca
      781 caagtgagcg cccactattg agaataaagc ccagcaagga gagatcctga tcggaaatac
      841 cttcacgcgc atcaatcacc aacatcacca cgttggcatc ttcaatggcc tgcaacgttt
      901 tgattaccga gaatttctct accgcatcgg tgattttgcc gcgtttacgt acgccagcgg
      961 tgtcaatgag cacatactca cgtccgtcgc gttccattgg aatgtagata ctgtcacgcg
     1021 tcgtgccagg catgtcgtaa acaacaacgc gctcttcacc aagaatacgg ttagtgagtg
     1081 tagacttacc tacgttcggg cgacccacaa tcgccagttt gatcggcaga ctttgcgggt
     1141 cgaagtcgtc ttcctcttct tcttcgccgt tctcttccgc ttcaaattgc gcccagtatt
     1201 cggcgtcttc gtcgacttct tcttgcggtg cgagatcttc catccacggc agcagcacgt
     1261 gctccagcag acttaatacg ccacgaccgt gagacgcggc gatcgggtag atttcaccta
     1321 aaccaagcgc gtagaaatca accactgcct gatcgggatc cagaccgtca gttttgtttg
     1381 ccaccaggaa ggtcggtttt tcacgggagc gcagatgttt agcaatcgct tcatccgccg
     1441 gcatcaggcc cgagcgcgca tccaccataa acagtacgac gtccgcttct tcaatcgcca
     1501 gcagcgactg ttccgccatg cgggtttcta caccgtcttc tgtgccatca atcccgccgg
     1561 tatcaatgca gataaactca cggccttcaa tttccgcacg accgtactta cggtcacgag
     1621 tcagacccgg gaaatccgca accagcgcat ctcgggtgcg agttagacgg ttaaataacg
     1681 tggattttcc tacgttaggg cgcccgacaa gcgcgaccac aggtaccatg tttaaagcct
     1741 catttttata aatcatcaga caacgcacgc tatattcgtg tcgttgttaa aaacaggaaa
     1801 acggcccctg tccaggagcc gttttcaaag tgaacgacag agacgattaa cgtgtaatag
     1861 agtacacggt tccgtctttt gcctggatca gcagtttgcc gtcagcggca accggttcag
     1921 tctggaaacc ggaactatca actttttgct gggcaacgaa acgaccatct tcgacgttaa
     1981 tccagtgcag ataaccttcg ctgtcaccta ccaccaggtt gccattatac agcaccggag
     2041 aagtcagcag gcgatgcagc agatcgcttt gtgtccacag cgtaacgccg ccatcaatgg
     2101 tcaacgccat cacccggtca ttttgatcga ccagatagat gcgattgccg tcgacgatga
     2161 aatcattcac cgaacccagt tcgcgtttcc acataatctg accgctgcgc agatcaagcg
     2221 ccgtcaggtt accattatag gccagcgcga aaacaacgcc gttaacaacg acgggagtcg
     2281 tgtcaacatc gctcagacgg tcaatttcgg tagaaccggt cgcctgggaa atacgctgct
     2341 gccaaatcat ctggccctgt tccatcagca ctgcgctgac gcgaccatta tcgccaccca
     2401 cgacggccgc accaaaagcc gttgccggcg cagactcgcc acgcaaagag agcgaaggca
     2461 tatcgaggtt aactgtccat ttgacagcgc cgtcagcttc gttcagcgct tgtaactgac
     2521 cgttactggt gtggattaac accagaccgt cgctgaccac cgggcgcgaa agtgcttcac
     2581 ccgcaacttt agtttgccat gccacagtac catcgctggt attcagcgcg taaacctgcg
     2641 ccttttcggt gccaatgtag acatgcccac cagacacggt cacgccgcca gaaagtaatg
     2701 caggatcttt agagaaccag ccatctttct cggccaggtt gacagaccag atttctttgc
     2761 cgtcatccgc attcagcgct tttactaaac cagcgcggtc cgctgcatag acaacgttgt
     2821 ccgccagtgc cggatgaaga ttggaataga agttgccaat gccgctacca acggaagtgc
     2881 tccacgcagt agtcggcgta aactggtttt caacggttgg caatggggac atctttacca
     2941 catcttcttc gctgttaaac agcgaacagc cgcttaaaag ggtaacggaa agcagtcctg
     3001 gcagcagtaa tttacgcaat tgcatcgggt ccctctcaga tggacaaatt attaattttc
     3061 atctgcatca tttcgctcag tgccggggta acatcgcttt tcacgcctgc ttcccatgca
     3121 ctacgcgcac cttgcttatc acctttgctc agcaatgctt caccacgcag gtcggcaaca
     3181 atggcagccc acccttcacc tttgatagca tcaagggttt ttagcgcggc atcagcctgc
     3241 ttgagctgta cctgaacgcg agcaagacgc agatttatca cggctttgag attttcatcg
     3301 ctcgtgtctg ccagcccctg ttgtaactgg gcggcagctt tctctagttc atttttgtca
     3361 acaaattgct gcgcaagttc caaagaagcc agcgcaccat aagtattttt attttcagca
     3421 gcaaattttt ccgccgctgg gatgctatcc ggtttgcctt cgctcactgc ggtaaccgca
     3481 ttttgatagg caagagaagc ggagcgtgca gaatcaacct gatggctgtt ccagtagcgc
     3541 cagccaatca gtgcgccaac gcccaaaatc accccaacag ccagtgcttt gccattttca
     3601 gcaaaaaagc gtttaaccgc ttcaacctgg tcgttttcgt tctcgtaaat ttccacgctg
     3661 tccttctcct tccttaaccc agtaacgtgc gcaaatgcgc ggctacgcta tcctgcgcaa
     3721 ctgcagtttg ctcaccagag cgcaaatcct tcactactgc tgtgccgtta gccacttcag
     3781 actcacccag caccacagca acgcgggcac cccatttatc agcacgggca aactgtttct
     3841 taaagttgcc gccgccgtgg ttggtcatca gtttcacgcc cggtaattca tcacgcagac
     3901 gctctgctaa tgccatagcc gcagattgtg tgtcagcacc tgaagccacc aggtatatat
     3961 cgacaacagg atcggcttta aattccggat taacggcctg aactaacaat acaagacgtt
     4021 cgaggcccat cgcaaaaccg actgccggtg ttgcacgacc acccagttgt tccaccagac
     4081 cgtcataacg accacctgca cacacggtgc cctgggaacc gaggctgtta gtcacccact
     4141 cgaaaacggt acggttatag taatccagac cgcgcaccag acgctggttt acggtgtaag
     4201 cgatccccgc gctctcaagc agtttgcaca gaccggcaaa gtgctcacga gattcctcgt
     4261 ccagataatc gcctaatgcc ggagcgtcgt tgagaagcac ctgcacttcc ggattttttg
     4321 aatcgagtac gcgcagcggg ttagtgtaca tgcggcgttt gcagtcttcg tccagctttt
     4381 ctttatgctg ctcaaggaat gccaccagcg catcgcggta attcgcacga gcttccagcg
     4441 aaccgataga gttcagctca agagttacgt gctcggaaat acccagcgcg cgccaccagc
     4501 gggcagtgag cataatcagt tcggcgtcga tatcaggacc ttgcagaccg aaaacttcgc
     4561 agcccaactg atggaactga cgataacgcc ctttctgcgg acgctcgtga cggaacatag
     4621 gcccgatata ccacagacgc tgttcctgat tgtacagaag accatgctcg atgccggcgc
     4681 gtacacagcc cgccgtccct tcagggcgca gagtcaggct gtcgccattg cgatcctcaa
     4741 aggtgtacat ctctttttca accacgtcgg tgacttcacc aatcgcacgt ttgaatagcg
     4801 gggtctgctc tacaatcggc aagcggattt cactgtaacc gtagctgccg agcacgtttt
     4861 tcagtgtgcc ttcaatgcgc tgccagatgg ccgtttcgcc aggcaggtaa tcgttcatgc
     4921 cgcgaatggc ttgaatgttt tttgccacgt ttattctctt tctgaatata aaaatgaacc
     4981 ctcaacgctt ccctcaatgt ttcgggagcc atgcgggttc aatcatacac gggaagcgag
     5041 gcgcttccca tcacgttatt atttttcaac ctgctgaacg tcaattcgac gcgcttcgtc
     5101 cagctgactg gctttcgcac gaatgcgcgc ttccagctga tcgatcatat cgttgttgtc
     5161 cagacggtct ttgcgcacgc catcttcata gaggccgctt ttcttgttgc cgccggtgac
     5221 gccgagtgta gaaaccagcg cctcaccagg gccattcacc acgcagccga taatcgaaac
     5281 gtccatcgga gtgatgatat cttccaggcg ttgctccagc gcgttaaccg taccgataac
     5341 atcaaattcc tgacgcgaac aggtcgggca ggcgatgaag ttgatcccgc gcgaacggat
     5401 acgcagcgat ttcaaaatat cgaaaccgac tttgatctct tcgaccggat cggccgccag
     5461 cgatacgcgc agcgtgtcgc cgatgccttc agacagcagc agacctaaac caatagcgga
     5521 ttttactgcc ccgctacgcg caccaccggc ttcggtgatc cccagatgca gcggctgatc
     5581 gatttgtttt gccagcaaac gataagactc aacagcgagg aagacgtcag acgctttcac
     5641 gctgactttg aactgatcga agttcaggcg atcgagatga tcaacatgac gcatggcaga
     5701 ttccagcaac gcctgcggcg tcggttcgcc atacttttct tgcagatctt tttccaacga
     5761 tccggcgtta acgccaatac ggatcggaat gtttttatcg cgcgcacagt caaccaccat
     5821 gcgaatacgc tcttcattac cgatattgcc agggttaata cgcagacaat cgacgccgta
     5881 ttccgctact ttcagcgcaa tgcgatagtc gaagtggatg tcagccacca gcggcacgtt
     5941 aacctgctgt ttgatgagtt tgaacgcttc tgccgcgtcc atcgtcggta cggagacacg
     6001 gacgatatca gcgccaacac gttccagcgc cttgatttga ttgaccgttg cttcgacgtc
     6061 tgtcgtacgc gtattggtca tggactgtac ggcgatggga gcaccatcgc caatcggcac
     6121 attcccaacg taaatacgtg ttgattttct acgttgaatt ggagcctggt tatgcatgaa
     6181 aaatctcccg cgttacccgt ctgttactgc gccggtgatt gttcggcatt gagggtcaga
     6241 cgcgcaacct ggttagttct gataaaacga ctcagatcga caggtttccc ttgatactgg
     6301 atctgtactg cggctggcgc accaattttc agtttgtacg gtgcctggcc ggttaagttc
     6361 aaattaccgt ctttacgctg cataccgcta aacagttttt taccggtagc atcggtgact
     6421 tccagccagc aatcggcagt aaagttcatc accagcgcat tcgggtcaac cgccggcgtg
     6481 gtcacgccag cctgatcggt tggcaacggc gcagcgccat ctggtgttgt tgttgccgct
     6541 ggtgccggag tcgcagcggt atcaacattt gcctgcgaag gtggaacaac cgcattctgt
     6601 tgcggatcaa cagctggtgc tggcgcagtt acggcaggtg tttgcgtgtt ggttgcggtg
     6661 gtatccacag gtgatgtcgg cgtggtcgcc atagcctgat ctgtggttgt cgacgtatct
     6721 aacggtacac tctggctttg attattattc agttccgcag aagattgatc ggccatagtg
     6781 gtgatctctt cctgctgagc tttgtggtct tgccaccacc aggcaccgct caggccaata
     6841 accacaaaca acaccagcca ggtgaaggtc atcagccagc cgtcacgttt tttgcggcgt
     6901 ttaccgaggg aaaaactctg catcggcgca acttttgcag cccgtagtgg agcctgcttt
     6961 tccagccctg gcagcagttc ttcttctgga atatgtacca gacgcgcata agagcggata
     7021 tatccgcgca ggaatgttga agcaagatcg gcgggtgcct tatcttcttc aatgtcgcgt
     7081 accgtggaaa ccttcaggca aagtcgctcg gcaacggcct gttgactaag tcctagttgt
     7141 tcgcgagcat tacgcaggcg agcgccggta gtaagtgctt cattttggtc gtgcgtggct
     7201 tcagtattca ttcgctgcag gtacgtttaa atgagaattg agatgccggt gaaccatcat
     7261 gcccacccac accgcgaaac atccgttaag ttaaccgtta tcatacagta taagaccgtc
     7321 tgtgtgctcg tgacaaagcc cacattcgct aaggctaact tactgttgca tcgttacata
     7381 ctgccttaaa gtcagcaaaa acgcaccgtt aatattgacc agacaattgc aacttaatac
     7441 ttcattcata aaagattacg ccacggtaca taaagtaacc gtggcgtaat ggctatcaga
     7501 ccgctttaat gtcgatggct tcaccctgca tccgcttacg cagagtacgt ttcgtacggt
     7561 cgataacatc gcccgccaac tgaccacaag cagcatcaat gtcatcacca cgagttttac
     7621 gcacaatagt ggtgaaaccg tagctcatca gcacttttga gaaacggtcg atacggctgt
     7681 tcgaactgcg tccatacggc gcgcccggga acgggttcca cgggatcagg ttgatcttac
     7741 acggcgtatc tttcagcagt tctgccagtt ggtgcgcgtg ttcagtgccg tcgttaacgt
     7801 gatcaagcat cacgtattca atagtaactc ggccctgatt ggcgttggat ttctccagat
     7861 aacggcgcac cgccgcaagg aacgtttcga tattgtactt tttgttgatc ggcacaattt
     7921 cgtcacgaat ttcgtcgttc ggcgcgtgca gggagatagc cagcgcaacg tcgatcatat
     7981 cgcccagttt atccagcgcc ggaaccacac cggaagtgga aagcgtgacg cgacgttttg
     8041 acaggccaaa accaaaatca tcaagcatga tttccatcgc cggaacgacg ttgttcaggt
     8101 tgagcagcgg ctcgcccata cccatcatca ctacgttagt gattgggcgc tgaccggtga
     8161 cttttgctgc gccgacaatt ttcgccgcac gccacacctg gccgataatt tccgataccc
     8221 gcaggttgcg gttaaagccc tgctgggcgg tggaacagaa tttacactcc agcgcacacc
     8281 ctacctgcga agagacgcag agcgtggcac ggtcgtcttc cgggatatac accgtttcaa
     8341 cgcgctgatc gccaacggcg atcgcccatt taatggtgcc gtcagatgaa cgctgttctt
     8401 caaccacttc cggtgcacgg atttccgcca cctctttcag tttgccgcgc aacactttat
     8461 tgatgtcggt catctcatca aaattgtcgc agcaatagtg atacatccac ttcatcacct
     8521 gatcggcgcg gaagggtttt tcacctaaat ctttaaaaaa ctcccgcatc tgctgacggt
     8581 tgagatccag caggttgatt tttccatctt tcgtagtgac gttttcaggt gtgactaatt
     8641 gttcagacat atgctattcc ggcctcgtta ttacacgtta tggcccctgg agggttgaaa
     8701 aaagaaacgc cccggtgagc ggctgctcgt ccgggggcgc tgcattgtac aaattctggc
     8761 gcacggatgc cacgtttgca cgcggcattt acgaaattat taacgggtgc gcgggcacac
     8821 ttcgccttcg ccgaagaaat aagcgatttc acgagcggca gattcgacgg aatcagaacc
     8881 gtgggtacca ttttcggtca ggctgtcagc gtaatcagca cgcagagtac cggccagtgc
     8941 gtttgccgga ttggtcgcgc ccagcagatc gcggtgacgc tgaacagcat tttcaccttc
     9001 cagcacggaa acaacgatcg ggccagaagt catgaattca accagaccat caaagaacgg
     9061 ttttccatcg tgttcagcat aaaagccacg agcctgttca acggtcaggt gcagcatttt
     9121 ggtgccgaca attttgaacc ctgcagcttc aaagcgcgca aagatactac caatcacgtt
     9181 ttttgctacc gcgttcggtt tgatgatgga aaaagtacgt tcaatagcca tttttacctc
     9241 tgtaaattgt tctgttgttg tctgtaccag cgtacgagat ggcgcggatt ataatgagca
     9301 acagagccgt tgactattga tgaaggtaac atttttttaa aataaaccca gtttaagcaa
     9361 caatcaggga caaaacagac tattgcatga caaatttcac tgtcgctatt tgccccacgt
     9421 catccatcac cagcaattga taatcgcctt tatccgtcaa atgcagggtc acgttgcgcc
     9481 cacgttcagt taacggttcg ccgttcagaa accaccagcg ttcacctgcc cctccacttg
     9541 attgcaacgg caaagtcgct tccgctgcgc ccggtaaacg tttaataatc gcgccatcgc
     9601 gcacacctgt tagctgcagc gggagttgtg catcgtgacc gtatggcggg caaattgtcg
     9661 aggccggtgg caagcgcaca gcgcgacgct ctgatgcggg cagccagggt tccagcggta
     9721 gcggccagac atttatcatt tcttgtcgcg cctgagggca atcagcggca acacgtttgc
     9781 cgttttcatc cagccagatg gggaaacgaa tgccattaat gccttcctgc tccggcagta
     9841 atagagttgg cggctggctc ccgtccagca gccaggttgc caggcggcgg cgacagttac
     9901 cgtcaccttc cggcaaagac tgtccgcccg gccagcagat aacgccacga cttacagagt
     9961 caggacgtgg gtcttccggc aaattcacac tgcgcgacag taagatatta ttaacctgat
    10021 tcaacagcgg tacggcactg gcaaagccaa actgaccaac aacgggcgtg ccgtccggtc
    10081 tgccagtcca gatcccaatg acatagcgag cgttaacccc aatcgcccag gcatcacgat
    10141 agccatagct ggtgcccgtt ttccatgcca gtggggcgac gcgcggcaag tcaccatccg
    10201 gcaagggttg cgcttcatca gccataatcc ggcgaatgat ccacgcagcc cccgacgaca
    10261 ttaaaggccg ttcaaacagc ggatcgtcag gctgtaagcg caatttgcct gccttgccgt
    10321 ggcgagcaaa cgcggtatac gctgccgcca tatcttccag ttttgcgcca gcgccgccga
    10381 gaatgagcga aagattcggc gcagcaccgt tgggcagata caacggcaat ccaacattgc
    10441 gtaactttgc cgcaaaccgt ttcggtccat aggcttccag cacctgcaca gcaggtaagt
    10501 tcagcgatcg caccagcgcc tcactcatgc tgatcgggcc atgaaaaccg ctgtcaaagt
    10561 tgccaggtcg gtaatcaccg gtgcgtcggg ggacgtcttg caacagcgat gccgggtgga
    10621 tcaagccttc atccagcgcc agaccataaa caaacggttt gagcaccgat cctggcgatc
    10681 ggatcgcatt gaccatatca acatgaccaa agcgtgaatc atcgttgaga tcaaccgatc
    10741 ccacccagcc gcgaacacgc atatcggtat gatcaaccac gatcatcgcc agtgagctgc
    10801 gcggtggcaa tcgtcctttc cagttttgcg ccagttcttc cagacgtcgt tgaagaccgg
    10861 catccagcgt agtaacgatt ttgtcgcttt tgcttttacc gagcatcatg cgcgaaaaca
    10921 gcggtgccag ttgcggcatt tgtcggggtg ccagccagat gggttcttcc cttgactctt
    10981 ttacctgctc acgggaccac acaccttgca cggccatccg ttcgagcact ttattacgcg
    11041 cggcttcggc acgctccggc caacgatccg ggcgaagacg gctgggcgct tgtggcaaca
    11101 ccgccagcat cgccgcctcg gaatagctta aattcgcagg cgatttcccg aggtaagccc
    11161 aacttgccgc accaatcccc tgcaacgtac cgccaaacgg ggcacggtta agatacaagg
    11221 tcagaatttc acgtttagac agatgccatt ccagttgcaa cgcgcgccag agctggcgaa
    11281 ttttgccgcc aaacgttttg gggtgaggat caagcaggcg cgccacctgc atggtgagcg
    11341 tgctgccgcc ggaaatgacc cgacccgacg tgagatcttg ccaggctgca cgcgccaccg
    11401 agaacggatt cacccccgga tgcatccaga accagcgatc ttcataattg atcagcgctt
    11461 caaggtaacg tggagaaaca tcttcgattg ttaccggata acgccagatg ccgtcagcat
    11521 cggcgaagcg ccagagtggc gtaccatcct gcgccacgac cactcgtgcg ggattgactt
    11581 catgcagcgg tagaggccag agtttatctg ctccccacgc tgcaagaaaa aggagaaagg
    11641 gcgcagctgc cagcgttatc cagcagccgc gtttggttaa caggcgaggc atttacggtc
    11701 tgacaatcag caaatcttca gccgcgccgg tcgcccgcca ttggggaaca tacattgatt
    11761 ccaccatcgg ttgcggtacc tgatacgttc ccggcgtcac cgcccgcgcc agatacacca
    11821 gcgttaccgg ttggtattca tcaacggcaa ccgccgccac aaagcgatcg tcacggaact
    11881 ctatgtgctt aatgctcgcc tgctgcatct ggttcagcaa gttctgcact tcaccaccac
    11941 tttgctccag gctggcgctg ccgttcgcca gattctggtt ttccagttcc agccccgcag
    12001 gcaacagatc cacgactaac gcatccggta cactgttact ggctttaacc tgcaaccaca
    12061 ccagcaccag atcgccgcta cgtaacgagt ccagcgattt gctcttaccg tcagtaccca
    12121 gaatatgacg ctcaatttgc agcacattgc tcgccggtaa aggtgcggat tgcggataac
    12181 cgctggcatc cacacgcagc cataacggct gatcgccact gttggtcacc tgcaaggtgg
    12241 caagttgatc gctattcaga ttgctggttt gcgctttctc gcctgtcagc ggctcagcag
    12301 agaaagtggt ttgcgcctgc catttgcccg gtaaatcctg aagagtacgg gcagccaaga
    12361 acaacgcgtt actttcctgc gtcgatagcc agcgttcacc gaacgcctgc tgcgaaagtg
    12421 tgttcaacag ggagtactgc tcatcgggta gcagtttgtt ctcttccagc aatgagagca
    12481 tcaacgcgct gtcgcgcagt ggactaccgt aatcacccag ccatatccgc tcatcactat
    12541 tacgcggcgt tttcagcgcc agcacaatcg cttcttcacc acgcattgca tcacccatgg
    12601 ttttcagcgc aacgccaagt tgcagcagcg gtaaaccaga agcggcatct gcacgatgct
    12661 cccagatttc acgcagcgca cccagtggtg ctttttgctg acgggccaac agcagcgcag
    12721 cgtaagactg tacggcgaat ttactggctt tgagattatc agcgtacggg atcgacatca
    12781 tgcccggatc ttgtaaatag cgcagcagac gctcattacc ccggttaatg gcgtctgtcg
    12841 gcacgctgta accctgttcg cctgcgcgga ccaggaaatc catcacgtaa gccgtcagcc
    12901 agtactcttc gtcaccgttt ttatcccaca gcgcaaagcc gccgttatca cgttgcattt
    12961 gcagcaaacg ggaaatgccg atatcgaccg atgcacggcg tttctcatca ctgtcgcctt
    13021 tgatgcccaa cgcctgcagt tgggctgcat tggtataaag tgacgggaac aggccgctgg
    13081 cggtttgctc gagacagccg tacggatacg cttttaactc tttgatataa cgtgcgatgt
    13141 tcagcggtgg tttgccgctc aacaacaatt gcccttccag cgtaacaggc gagaagtttt
    13201 gcaatccatc agccgggagc gcccaggttt caccaggctg taacgccgta ccgtaattga
    13261 ccgtttgtgc cgggaatgcc ggacggacgc cgattttcca ctgcttatgc tgatcggcaa
    13321 cggtttcccc cggtaacgct aacccgctaa tggtggcctg aatgtctcca tcgccataac
    13381 ccggcaatgc acgcaccggg ataaacagcg tagtacgcac ccctggcgct aattcaacag
    13441 gtgcggggga attactcacc agttcaagca agccactggc ggtcagagca acattaagtt
    13501 tttgcggttt atcggtaaga ttagtgatat ccagcgtcag gcgcgaggta tcgccactcg
    13561 ccataaagcg cggcatgttc agttcagcaa tcaccggtgc ggcaacaatc actttgcttt
    13621 cgttgcttcc gaagtcatct gccgtccagg cttgcgccat gacgcgcaat tcaccgttaa
    13681 aatcgccaat tggcagtgta accgagcctt cgccctgttc attgagcgtt accggcagcg
    13741 cctgctgcgc gacaatattg acgtgattga ccggcggttt accaccacgt ttcagctcat
    13801 caccatcgcc accaaaacgc agagctgcca gacgcccctg accttcaatg acctgaccgt
    13861 aaatatcgta aatgtctgcg ccatagcgtt tctgaccaaa gaacgcttgc cacggatctg
    13921 gcgtgacgta atcagtaata ttcagcacac cgctatcaac cgcagacacc agcacattca
    13981 cctgttttgg catctcacca tttttattgc tggctttaat tttcacggtt aatggctgat
    14041 tggggcgcat tttggtaggg gtttccagcg ccagatcgag gcgacggttt tcatcaccaa
    14101 gcggcagatg caacacccca accgcgcgtt ttggcgtcgc ggagcgagat ttatcgccag
    14161 gacgtaccac cagcgtactt aaatacagat catgacgatt ccaggtttta tcgaccggaa
    14221 tcgtcagatc cagcccttga gccggaacat caatctcttg ccaccacagc ggcccttcac
    14281 tggactcgac catcgcataa cctttacccg ccgttggcgc ggcaatatgc aacttaatgg
    14341 tatcgccagg gcgatagctg gctttatcca gtttcagcgt gacacggtcg ggtcgcactg
    14401 caccaccccc gtcgctgtta tcctgccagc tataaccagc ccagaaacgc acactactga
    14461 ccgcttcatt cggcgcttta acttccagac gataagcgcc ccactctacc gggaaactga
    14521 ccttaccggt ttcgtccgct ttcagatcca gagtttgttc attttcgatc agatcttttt
    14581 gatcaaactg tgactgccag ccttcatatt ccgaccagtt ccagtagtaa tcgcggcgtt
    14641 cgcgaatcag gcgcacctgc aagcccgaca cggctttttt cacgccttgc gcatcgctat
    14701 aaacgatgtc aaaagcggcg ttactacctt catcaacaat gggctgtttc accgtgctgt
    14761 cggtgcgata atcgtaaacc gatttcgagg cgaactgcgg acggatccct ggcaatgcat
    14821 cagcaggcca gatagcctgc tcagcacggc gcgtcaccgg gcgaccgccc gattccagca
    14881 aactaccctg gaaaataacc tgtaatgggg aatgtgtttc cttccactgg ctttctgtag
    14941 aaacttcgcc gcgcccttta tcatccagcg tcaactgaac ttcatccagc gtgcgggaaa
    15001 gattttcggc agctatatcg ccgaattcaa aaccaggtaa ggctgacaca gcttcacgca
    15061 gtgggcgcag gaaaagttgc ccttgcaaag tattaccatt agcaggtgca ccatacaggt
    15121 agtaccccac cacggagaat ttcacttcat ctttcggcgt tagcggggtt ttctcaccgg
    15181 tcagattcag cgccatacgc tctggcataa aatcttcgac gtggaaatcc cacatccgat
    15241 actggttatc gcccgtgtta gcgcgaatat gccacatacc ggttgccgca ttgctatcga
    15301 gtggccaggt aaagtggtag aggccattct ccggctgact aacgacgctc ctgagtacct
    15361 gcccgtcggg tttaatcacg tctaacttga tgggctgatc gggcaatgct ttaccgtctg
    15421 catcacgcaa taaaccattg aggatgactg tttcacccgg gcgataaaga tcgcgcgggc
    15481 caaacatgaa aaactgtttg ctatagcctg gcgcgccagc aatgttaaat tctgctaagt
    15541 ccagcgccgg aagttttaaa tcgagtagcg ttgtctgacc gtctttacgc gccaacagta
    15601 atgccgcgtt tttatcattt tccagctgca catgcccctg ggcatcactg gttgcctgag
    15661 tcagagtctg ccctttctca tttaataatg agacttcaac tccttgctgg gccgcgccgt
    15721 tttccaggct ttgggtaaag atatccagac gattgtgata acggtgagct gaaacgccga
    15781 tatcacttaa cgtaaacagc gtcgcgggat tactgtaatc gtaacgtcca gcctgattca
    15841 tcacagccag atacacgcct gcctgctgaa gcggtttgat atcgcccagc ggcagcaata
    15901 atttttcacg ggtgttacgc gcaggattga gatcaaaccg tccggtataa accagatccg
    15961 ccatctgcag cagtttgtca gactgccagt tcgccagcga attgcggtat tcccattggc
    16021 taatgaatgc aggcagagat tctggcttaa cacggaagaa gttaacatcg acattgttaa
    16081 cgttgagcgc cattaccggc agcccttcaa taactttacc aggtaacaac gagccacggc
    16141 tggcaaaacc gacgctgggt tggatatcgc gggtagttat agttttttcg taatctttac
    16201 tgaaggttgc gttgttgagt gctttgacct ccttgccaat agtgacgatc aaatcacgtt
    16261 tcggttcgag gtggcgtaaa cgcaactctt taagattatc tgacagctcc caggcaccat
    16321 ccactttgcc gcttttctta tcgacgacat ggataacgcg tgagaattcc tgatccgggt
    16381 cgagagggat ggagaatgtc agcaccagcg tggctgcgcc atcaagttga acttcagaga
    16441 gatccagcaa tgtcagcgcc ttaccggcac tctgttgggc cagtttttgt cgctctggcg
    16501 cggaaagttt tgctgaactc acgttttctg aagaggccgc tttagtaact tcagaaggcg
    16561 catctttttt caccgctgtt ggcgcgttat cgttgttgtc gcaccctgcc agcgccagca
    16621 ttagcatgca ggcggctacg cgtaactttt tcatttttca tccctgccac aatggcccgt
    16681 tagcaacgtc gaataattat tatgcgtgag aatttacgtt atgtaattca gtatcacgcc
    16741 catacaatcc gaattttagt gagaattgtg gctcagacag ccaaatcagc ctcttttgct
    16801 tgtcgctttt cactaaacat ccgacaattt agcctaaccc cggcaaaaat ggagatgcct
    16861 atgtccacga catggtttgt aggagccgac tggctcgccg aacatattga tgacccggaa
    16921 attcagatta tcgatgcccg catggcgtcg cctggacagg aagatcgtaa cgttgctcag
    16981 gagtatctga atggacatat tcccggcgca gtgttttttg atatcgaagc gctttctgat
    17041 cacacttccc cgcttccgca catgctgccg cgcccggaaa cgttcgccgt ggcgatgcgt
    17101 gaattaggcg ttaaccagga taagcacctg attgtctatg acgaaggtaa tcttttctca
    17161 gccccacgag catggtggat gctgcgcacc tttggtgtag agaaagtgtc gattctgggt
    17221 ggtggacttg caggctggca gcgtgatgat ctgctattag aagaaggcgc agtagagctg
    17281 ccggaaggag agtttaacgc cgcgtttaat cctgaagccg tggtgaaagt aaccgatgta
    17341 ttattggcaa gccatgaaaa tacggcgcaa attattgatg cccgcccggc tacacgtttt
    17401 aacgcagaag ttgatgaacc tcgcccaggt ttacgtcgcg gacatattcc cggtgcattg
    17461 aacgttccgt ggacggaact ggtacgtgaa ggcgaactaa aaacgactga tgaactggat
    17521 gcgatatttt ttggtcgcgg tgtcagctac gacaaaccaa ttatcgtcag ctgcggttct
    17581 ggtgtaacag cagccgtggt tttgttggca cttgcgacgc tggatgtgac aaacgtgaaa
    17641 ctgtacgacg gcgcatggag tgaatggggc gcacgggcag atttaccggt tgagcccgta
    17701 aaataagctt tttacaggca ataaaaaacc gccgaatttg gcggtttttt attgctggtc
    17761 tggttcgcgg cctttccagc aggttgtatt accgtagtaa tgcaagcgcg tctcagcgga
    17821 cacaatactc gccagtaact ctctttttgt caagtaaaag agagttatta ttgttctgtt
    17881 agtgtattat ccactgcggc cctttccgcc gtctcgcaag cgggcgctgg cttaaggaaa
    17941 ggatgttccg cgaccgttag ggcaagcgcg tctcagcgct tgctatcgcg gcaatatcgc
    18001 cagtggtgct gtcgtgatgc ggtcttcgca tggaccgcac aatgaagata cggtgctttt
    18061 gtatcgtact tattgtttct ggtgcgctgt taaccgaggt aaataataac cgaagtctct
    18121 tcggcgacaa tttactggtg gttaacaacc ttcagagcag caagtaagcc cgaatgccgc
    18181 cctttggggc ggcatatttt agattatccg gttctgttta aagtcacgca aaaaaccacc
    18241 ccagcgacgt tcatagaatg gcgcaatatg ttcggtaata aagtggctaa ttcctttttc
    18301 cccttttttc acctgacaaa tatcgatggg ttcatcgcca ggtaatgtat cggtcgctac
    18361 acttcccgcc gcctgaataa tttcttcgat atcaccatcg gcttcaatgc caataagtaa
    18421 attaggctgt gcctcttcgc tctctttaat taaacaaata aaagcacgct tcactggctt
    18481 aatggtttta aataaggtgg tgagtgaatc aatcatttgt actggcggct ctgcgacttc
    18541 cgataatatc agcgattcac cgccttccag tacttcctgg ctactcagcg gattcccttc
    18601 ttcaccaatc aacaaactga tttcacgcgg cataaactct ttaccggttg gcagtttggc
    18661 attaaggaag agcgtttcgc caagggtcat ctcaaacaat gtgcgaacgg gcattacgac
    18721 aaatgcctgt tcgtcttcaa ccgcctgttg aagtgcttct aacgaggtaa aaaaaggaat
    18781 gacgctggtg ccgtcttctt tttcccagtg ctgtaaatca agcgcgctat cttcaactac
    18841 tgcctcgccc tgcgccgccg taccaggcac ccagacggtg gattccagta gagtacggaa
    18901 aaaggccggg cggtgagccg gttcggttgc tgctttttcc agcaggtctt caagttcgtt
    18961 ttttgtttcg gacataagaa ccacaattca ttcaacgttc ggggagcaag tgcctgatgc
    19021 gccacgctta tcaggcctac aagggagtcg caatctattg gattttcgca atttgtaggc
    19081 cggataaggc gtttacgccg catccggcat attagtttac gccgttaaca gattagcaat
    19141 cgtgcgcaca ccaagtcccg tagcacccgc agaccactgt tcaaccggcg ctttacggta
    19201 agtcgccgag cagtcgatat gcagccagcc ttgctgatag ttctcaacaa agtgcgacag
    19261 gaagcccgca gccgtacttg cacctgccgg atacgccgcg cttccggtat tgttcagttc
    19321 ggcaaagtta gacggcagct ggctgcggtg gaattccgcc agcggcagac gccagaacgg
    19381 ttcgttctct tgtgaggcac tcgccagcaa acgaccggca agcgcatcgt caaaactgaa
    19441 caacgcgtga taatcattac ccagcgcagt tttcgccgcc ccggtgagag tcgccgcatc
    19501 aatgatcagt tccggtttct gcgcactggc atcaatcagg ccatcggcca gcaccagacg
    19561 cccttcggca tcggtgttca tcacttcaac ttttttaccg ttgcgatagg taataatatc
    19621 gcccagtttg aacgcgttgc cgctgatcag gttatccgca caacagagga acagcttcac
    19681 gcgcttgttc agtccgcgcg taatggcaaa tgccagcgcc ccggtaaccg ttgccgcgcc
    19741 gcccatgtcc gacttcatcg agtccataaa cgcagtctgt ttgatgctgt agccgccgga
    19801 atcaaaagtg atgcctttac caaccaggca cgcgtacact ggcgcttctt tatcgccagt
    19861 tgggttgtaa tccagcgcca gcaataccgg agaacgttct gaaccgcgtc cgaccgtgtg
    19921 cagccccata taaccttgat cacgcagatc ttcgcctttg gtgatccggt aagtcacacg
    19981 atcgcccgcg acattgctga tcagatcaac agcacgctgt gccagttgcg atggccccag
    20041 ctcttccgcc ggtgcgttga tggtgtcacg cacccagtcg atgatcatca ggcggttatc
    20101 cagttcctgg cgctggacat cgtccagatc cggccactcc actttacgcg tgcctttcgg
    20161 ggctttgtaa ccttgccaga atgcccagca gcgatccgca tcccagcctt ctccgcttag
    20221 ctgaacatgc ttgatgccca gaccatcaat cttgcgcgcc gcacgctgga taagccccag
    20281 atcgtctgcc ccgttcaaat gcagggtaat gccatcatta ttaatgctgt aagttgcttt
    20341 ttctccccag cgcgcatcgg caggttgggt agagagggta attttcatcg cttctgtcat
    20401 tttagttatc cttatttgta aaagggccgc cagttggcag ccccaggatt tttccgctaa
    20461 atgattcgcg ttgcaggaag gcggcaagtg agtgaagccc caggagcata gataactatg
    20521 tgactggggt gaacgagcgc agccaacgca tctgcggcgt gaagcatgac gcggaaatca
    20581 ctcggcttcg tccagccaga ctaacaaaat cgcttcgagg attttctcgt tcgatgcctg
    20641 cgggtcgtcg tcgaaatctt ccagatcgca aatccactga tgcatatcgg tgaatcgaac
    20701 cgttttcgga tcaatatcgg gatacgcatc gtacagtgct tcgccaattt cgcggctatc
    20761 ggtccactta agtcccatac taacctctgt taatgctcac gcgcatggtt gatagtgtaa
    20821 cgcgggattt caaccactaa atcttcgtcg gtgacgcgcg cctggcagct taaacggctt
    20881 tccggctcca gtccccaggc tttgtccagc atgtcgtctt cctgctctga gctttccggc
    20941 agcgagtcaa aaccttcacg aacgatgcag tggcaggtgg tgcaagcaca ggatttttca
    21001 caggcgtgtt caatctcgat accgttacgc agcgcaacgt cgagaatggt ttcaccgcta
    21061 ttcgcttcca gaacagcgcc atcagggcag aggtcctgat gaggcaaaat aacaatcttt
    21121 ggcatattaa acctcgtcca cggaatggcc tttcagcgca cgacgaaccg actggtccat
    21181 gcggcgagcg gcgaaatcct gggtttgttt gtctacgttt ttaatcgctt gttcgatggc
    21241 gtcaacatca tcgccctgcg ccacttcact caggtgagcg gcagcatcgt cgatagcctg
    21301 acgttctgcg gcgcttaaca gcgcggcatc agcagccagc gcaccgtgca gactttccag
    21361 cacacgcgcc gcttctactt tttgttctgc cagcatacgg gcttttacgt cctgctcggc
    21421 atagctcatt gagtctttga tcatcgaggc gatttcgctg tcggtcaggc cataagacgg
    21481 tttgacctga atagatgcct caacgccggt ggatttctcc atcgccgtca cgctcaaaag
    21541 accgtcggca tcgacctgga acgtcacgcg aatatgcgcg ccgccagccg gtagcgccgg
    21601 aataccacgc agcgcaaaac gcgccagtga gcggcagtcc tgcaccagtt cgcgctcacc
    21661 ctgcatcaca tggatagaca tcgccgtctg accatcttta aaggtggtga aatcctgggc
    21721 gcgggctacc ggaatcgtgg tattacgcgg gatcactttc tccaccaggc cgcccatcgt
    21781 ttcgaggccc agcgacagcg ggatcacatc cagcaacagc atttcgctgt ccggcttgtt
    21841 acccaccaga atatccgcct gaatcgccgc gccaatagcg acgactttat ccgggtcgat
    21901 agaagtcagc ggtggacgac cgaaaaattc gcctactcgt tcacgcacca gcggcacgcg
    21961 agtagaaccg ccaaccatca ccacttccag cacttcatca gcttctacgc cagcgtcttt
    22021 cagcgcgcga cgacaagcca gcaaggttcg ttttaccagt ggcgcgatca gttcgttgaa
    22081 ttgttcacgg ctgatttcgc cctgccagcc cgcaacattc acagtcacgg agtccgcatc
    22141 gctcagcgcg attttggctg caatggcggc atccagcagt tcacgctgaa cgcggttatc
    22201 gctacgatca ggaatgcccg cctgctcgcg aatgtaatcc gccagcagat ggtcgaaatc
    22261 atcgccgccg agcgcggaat caccgccggt cgccagcact tcaaacacgc cgcgacttaa
    22321 gcgcagaata gaaatatcaa acgtcccgcc accgaggtca taaacggcga tcacaccttc
    22381 ctgaccggaa tccagaccgt aggcgatagc cgcagcagtc ggttcgttaa gtaagcgcag
    22441 aacatgcaac cccgccagac gcgccgcgtc tttggtgccc tgacgctggg catcgtcaaa
    22501 gtacgccgga acggtgataa cgacaccatc cagctcgcct gccagggctt cagttgcccg
    22561 cgccgccagt gctttgagga tgtccgcaga aacgcgcacc gggttcagca gccccgccgc
    22621 cgtttcaatc atcggcaggc cattttcgct ggactggaat tgataaggca gatgcggata
    22681 gcgttgctgg atatcagcca gcgagcgccc catcaggcgt ttaacagaac taatggtgtt
    22741 ggcggtgtcg agcgctgcgt tagtacgcgc gtcataaccc accgaatgcc cttgctgttg
    22801 atagtgaaca acagatggca gcaggtgaca gccttcatgg tcagctaacg tttcggcctg
    22861 accgctgcgc actgttgcca ccagcgagtt ggttgtgccc aggtcaatac cggccgccag
    22921 gcgacgctga tgcggcgcag cactcaaacc aggttcacta atttgtaata aggccatgtt
    22981 tagcttccag aaattaaaaa tcgagcagtt tttcttcgag ttgttcggca ctgctccgca
    23041 gtttatcgag aaaacgcagc ttacgcacgg tatccgccgc cgcgtcccac gtctcgttgt
    23101 ctaactgttc aaccatcaac tgatggcggg tatcaaacat ctttttcaca cgtttgataa
    23161 agctttccag ccgcgcttca tctttcgcct gttcgatctc gtccagttct tcgcgcagtt
    23221 ccagctgttc catcaggaac gcggtgtcgc gcacagtatg ctgctcgctg gcgagatcaa
    23281 aaccgtgcaa agaaagcaaa tattccgcgc gcattaacgg atgacgcagc gtttgccagg
    23341 cctggttaat ggtcgcggat tgctgtacgg cggcgagttg ttccgcctgg cttccgctgg
    23401 cgaatttatc aggatgatac tgacgttgta gatcctgaaa acgcaggctc agcgcctggg
    23461 tatcgagttg ataacgggca ggcaagccaa agagggtgaa gtaatccata acattctcag
    23521 ggctgcgatc cgccgcgata acggcgaatc aggtaaaaca aaccccacgc gcaggcgacc
    23581 acggtggggt tatcggtatg cgcatcaaac gtggaagctt tcgccgcaac cacactcatc
    23641 tttgacgttc gggttggtga atttaaaccc ttcgttaagg ccttctttga cgaagtccag
    23701 ctgcgtaccg tccagaaatt gcaggctttt gccatcgacc acgactttca cgcctttgtc
    23761 ttcaaacacg atgtcttccg gcgtcggttc gtcaacaaat tccagtacat aagccatacc
    23821 tgaacacccg gaggttctca cgcccagacg caggccaaac cctttaccgc ggttagccag
    23881 aaaggtattt actcgcgctg ctgcactgtc gctcagtgta atcgacataa ccaaacctca
    23941 actcttattt tgcttcacgt ttgcttttat agtccgcaat ggcggctttg atcgcgtctt
    24001 ctgccagaat agaacagtga attttcaccg gcggcagttc aagttcttca gcaatatcgg
    24061 tgtttttgat cgcctgcgct tcgtcgagag acttcccttt cacccattcg gtgaccaggg
    24121 agctggaagc gatagcggaa ccgcagccgt aagttttaaa acgcgcgtct tcaatgatac
    24181 cttcatcgtt gactttaatc tgcaacttca tcacgtcgcc acaggccggt gcacccacca
    24241 tgccgctgcc gacgttctcg tcgttgttgt caaaggaacc cacgttacgc ggattctcgt
    24301 aatggtcgat aactttttcg ctgtaagcca ttataaattc tcctgattcc gataccgatt
    24361 aatgatgagc ccattcgatg ctgttcagat ccacgccctg cttgtacatt tcccacagcg
    24421 gagaaaggtc acgcagacga ccgatggatt tacgaactaa ctcgatggtg tagtcgatct
    24481 cttcttcagt agtaaaacga cctaaagaga aacggataga gctatgtgcc agctcgtcgt
    24541 tcagccccag cgcgcgcagc acgtaggacg gttcgaggct tgctgacgta caggcggaac
    24601 ctgaagaaac cgcgaggtct ttcagcgcca taatcagcga ctcaccttca acgtagttga
    24661 agctgacgtt gagaatgttc ggcgcaccgt gttccaggtc accgttcagg taaacttctt
    24721 cgatatcttt gatgccgttc cacagacggt tacgcaggcc gcgcagacgt tccatctcgg
    24781 tcgccatctc ttcttttgcg atgcgatagg cttcgcccat accgacgatc tggtgaacag
    24841 gcagagtgcc ggaacgcata ccgcgctcgt gaccgccgcc atgcatttgt gcttcgatac
    24901 gtacgcgcgg tttacgacgc acatacagcg caccaatacc tttcgggcca tagattttgt
    24961 gaccggagaa agacatcagg tcaactttca actggctcag atcgatcggc agtttgccca
    25021 cgctctgggt tgcatcaacg tgatagataa tgccacgagc acggcacatt tcgccgatag
    25081 ccgcgatatc ctgcaccacg ccgatttcgt tatttacgtg catgatggaa acaaggatgg
    25141 tgtcatcacg catcgctgct tccagttctt tcaggtcgat aatgccatta cgctgcggtg
    25201 ccaggtaagt gacttcaaaa ccttcgcgct ccagctgacg gcaggtatcc agtaccgctt
    25261 tatgttcggt tttactggtg atgatgtgct tgcctttttt ctgataaaag ttggctgcac
    25321 ctttgatcgc caggttgtca gattcggttg caccagaggt aaagacgatt tcacgcggat
    25381 cagcgccgac cagatcggca atctgattac gggcgatatc taccgcttct tcagcctgcc
    25441 agccgaaacg gtgagaacgg gaggccgggt taccaaaggt tccgtccatc gtcataaact
    25501 gcatcatttt ctcggcaaca cgcgggtcca ccggcgtggt tgcggagtag tcgagataaa
    25561 tcggtaattt cattgctcta taaactccgt acatcactca atgcaaggaa ccaggctacc
    25621 ggctggatgt acgaccgtgt ttacggagtg tttagcactc cggcctgatt ctgaattctt
    25681 tttattaagc gcgtaactta acgtcgatcg cgtcttgtgt gcgggtgcgt ggcgcgtcgt
    25741 gagtatgctg acgaccagac acatccagca cttcctggtt attaaccagt tcgcctaaag
    25801 taatgttatt gagaaaaccg gtaagacggt cgctcaaatc acgccacagc gcgtgggtca
    25861 ggcatttatc gccgccctgg cagccgcctt taccctgaca acgggtggca tctacagatt
    25921 cgtcaacggc gctaatcact tcgccaacgg cgatgctgct ggcatctttg cctaacagat
    25981 aaccaccgcc tggtccacgt acgctggaaa ccagaccatt tttacgcaga cgggaaaaca
    26041 gttgttccag ataagaaagg gaaattccct gacgttcgga aatatcagcc aacggtaccg
    26101 ggcccgcttc agagttgagc gcaacgtcaa gcattgcggt cacggcatag cgccctttag
    26161 atgtcagtct catgtcttac ttcacctcaa actcgcccct gcccggggtt ttttattgta
    26221 aaagtggggg tattgcatag cagggtcaag tctgacattc ccgagtaaat tggtcaacta
    26281 tttacttgac tgatttagtc gggtatttaa ccttcagtgc cattttttat cgtggcgttt
    26341 gtacctgttg tcggatgcgg tgtgaacacc ttatccgacc ttggattccg tgcacttaca
    26401 ggcctgataa gacgcgttaa gcatcgcatc aggcaaccac tgttattcgg ccttgttacc
    26461 tttattctgc tgctcaatag aagccagaat cccgcgcagg atattcaatt cctggctttc
    26521 cgggcgtgca cgggtaaaca gacggcgcaa tttattcatc acctgccccg gatggttttc
    26581 acggataaaa ccggttgcca gcagcgtttg ctccagatga ccataaaaac gctccagatc
    26641 atcgaccagc ggatacggcg tctcttcatg ctcgacctgc tcgccatttt cctgagttgc
    26701 cagccaggcc atgcgcactt cataggcaat aacctgaacc gccatcgcca ggttcagcga
    26761 actgtattcc gggttagccg caatcgcgac atgataatgg catttctgca actcttcatt
    26821 ggtcaagccg acgcgctcgc gaccaaacac cagcgccacc ggggtatttg ccgcttcagc
    26881 gacgcttttc agaccgcatt cgcgcgggtc gagcatcggc cacggcagcg tgcgggagcg
    26941 agcgctggtg cccaccacca gactacaacc ggctaacgct tcgtcgagcg tatcgacgat
    27001 gtgagcatta ccaatcacat cgctggcccc tgctgccagg gcaatcgcct gggagtcagg
    27061 tttcactagt ggattaacca gccacagatt ggttaatccc attgttttca tggcacgggc
    27121 aacagaaccc atattgccgg tgtgtgacgt ctccaccagc acaattcgaa tattttgcag
    27181 cattgtcttt cttcatctaa agattattca cgcatcttat cataaaacga agacagatgc
    27241 cgatctcgct gctatactct gcgccgtttt cccgttcttt aacatccagt gagagagacc
    27301 gatgcatccg atgctgaaca tcgccgtgcg cgcagcgcgc aaggcgggta atttaattgc
    27361 caaaaactat gaaaccccgg acgctgtaga agcgagccag aaaggcagta acgatttcgt
    27421 gaccaacgta gataaagctg ccgaagcggt gattatcgac acgattcgta aatcttaccc
    27481 acagcacacc atcatcaccg aagaaagcgg tgaacttgaa ggtactgatc aggatgttca
    27541 atgggttatc gatccactgg atggcactac caactttatc aaacgtctgc cgcacttcgc
    27601 ggtatctatc gccgttcgta tcaaaggccg caccgaagtt gctgtggtat acgatcctat
    27661 gcgtaacgaa ctgttcaccg ccactcgcgg tcagggcgca cagctgaacg gctaccgtct
    27721 gcgcggcagc accgctcgcg atctcgacgg tactattctg gcgaccggct tcccgttcaa
    27781 agcaaaacag tacgccacta cctacatcaa catcgtcggc aaactgttca acgaatgtgc
    27841 agacttccgt cgtaccggtt ctgcggcgct ggatctggct tacgtcgctg cgggtcgtgt
    27901 tgacggtttc tttgaaatcg gtctgcgccc gtgggatttc gcggcaggcg agctgctggt
    27961 tcgtgaagca ggcggcatcg tcagcgactt caccggtggt cataactata tgctgaccgg
    28021 taacatcgtt gctggtaacc cgcgcgttgt taaagccatg ctggcgaaca tgcgtgacga
    28081 gttaagcgac gctctgaagc gttaatgact caggcgggtg atatcactca cccgccctcg
    28141 cctttcaggc gctattccga aatacttcct caccgcttta ctttctttcg tcactctccc
    28201 accatctttc cccgattaat ggatgataaa gaagtcgcaa ggaaaggtta tggcgctgcc
    28261 agtgaacaaa cgcgttctca aaattctgtt tattctcttt gttgttgcct tctgcgttta
    28321 tttagtgccg cgcgtcgcca tcaacttctt ctattatcct gacgataaaa tatacggtcc
    28381 cgacccctgg tcggcggaat ccgtcgaatt tacggctaag gacggtactc gtctgcaagg
    28441 ctggtttatt ccttcttcga cgggccctgc tgacaacgcc atcgcaacca tcattcatgc
    28501 tcacggcaat gccggaaata tgtccgccca ctggccgctg gtcagttggt tacccgagcg
    28561 taatttcaac gtttttatgt ttgattatcg cgggtttggt aaatcaaaag gcacgccgtc
    28621 ccaggccgga ttgctggacg atacgcaaag tgccatcaat gtggtgcgcc atcgcagcga
    28681 tgtaaaccca caacggctgg tgctgttcgg gcagagtatt ggcggggcga atattctgga
    28741 tgttattggt cagggtgatc gtgaaggcat acgtgcggtg atcctcgact ccacatttgc
    28801 ctcttatgcg accattgcca accagatgat ccccggcagt ggctacttac ttgatgagag
    28861 ttacagcggc gaaaattaca tcgccagcgt cagcccgatc ccgcttttac tcattcacgg
    28921 taaagctgat cacgttatcc catggcagca cagtgaaaag ttgtatagcc tggcaaaaga
    28981 accaaaacgg ttgatcctaa tcccggatgg cgaacacatt gatgcttttt ccgatcgtca
    29041 cggcgatgtt tatcgcgaac agatggtgga ttttatcctt agcgcattga atccgcagaa
    29101 ctaacccatg atcgctagca cgataatcat tcacaaaacc accttaagac atgctaatcc
    29161 actggtcaga acagtttaag atgagaaaaa ttctgtgatg cttgccaaca tttctgatga
    29221 ttagcattcc cttcgccatt tccctgagca aactttagct attcttatca attatgctta
    29281 tgggagatct acagatgatg cctacgcttg ctccaccatc tgtcctttcg gctccccagc
    29341 gccgctgcca gatcttgctg acgctctttc agccggggtt gaccgccacc acggcaactt
    29401 tcagcgagct taatggtgtg gatgatgata ttgccagtct tgatatcagc gaaacaggac
    29461 gggagatcct gcgctatcat caactcacac tgactactgg ttatgacggt agctaccggg
    29521 ttgaaggtac agtgcttaac caacgtttgt gtttatttca ctggctacga cgtggtttcc
    29581 gtctgtgtcc gtcatttatt accagccatt tcacccccgc cctgaagagt gaactgaagc
    29641 ggcgcggaat tgcgcgtaac ttttacgacg ataccaatct acaagcgtta gtgaatctct
    29701 gctcccgacg gctgcaaaaa cgctttgaaa cgcgcgatat tcatttcctg tgtctgtatc
    29761 tgcaatattg tttgctgcaa caccacgctg gaattacgcc tcagtttaat ccgctccaac
    29821 gtcgctgggc ggaatcctgc cttgaatttc aggtagcgca ggaaattgga cgccactggc
    29881 agcgtcgggc actccagcct gtaccgcctg atgagccact gtttatggct ctactgtttt
    29941 ccatgttgcg ggttcccgat ccactgcggg atgcgcatca gcgggacaga caactgcgtc
    30001 agtctatcaa acgtctggta aaccattttc gtgagctggg aaatgttcgt ttttatgatg
    30061 aacaggggtt atgcgatcag ctttataccc acctcgccca ggcgttaaat cgcagtttgt
    30121 ttgccatcgg tattgataac accctgccgg aagagttcgc ccgcctgtat ccacgcctgg
    30181 tgcgcaccac ccgcgcggcg ctgaccggat ttgaaagtga atacggcgtc catctttctg
    30241 atgaggaaag cggtctggta gcggtgattt ttggtgcttg gctaatgcag gaaaacgacc
    30301 tgcatgagaa acagattatt ctactgaccg ggaatgatag cgagcgagaa gcgcagattg
    30361 agcagcagct acgcgaacta acgttactgc cgctcaacat taaacatatg tcggtaaagg
    30421 catttttgca gacaggcgct ccgcgcggcg cggcactgat tattgcgcct tataccatgc
    30481 cgttaccgct cttttcacca ccgctgatct atacggacct gacattgaca acacatcaac
    30541 aggagcagat ccgcaaaatg ctcgaatcag catgagggaa caactttcgg gcgcaaaaac
    30601 attgccggaa gcgccaccag cgccatcacc cagaacacgc cgtggccgag atattgatac
    30661 aggaaaccgg cgaaaacggt catgatggcg atactgccgc ccatcgcgac ggcagagtaa
    30721 accgcctgta aacggatgac ttcgctaccc tggcgagcag caatatagcg catggcagcc
    30781 aggtggcaga ccgtgaaggt accgcaatgc agaatttgca ccactatcaa ccacggcaac
    30841 gccgtcgttg ctcccataat gccccagcgc actacgccgc aaatcgccga gatcaacagc
    30901 atatcgcgtg cactacaacg gcggaaaagt ttattactca gcgcaaagat aatgacttcc
    30961 gccaccacgc ccagcgacca caaatacccc accgccgagg ccgagtagcc agctgcctgc
    31021 cagtaaatgg cgctaaaacc gtaataggcc gcatgtgccc cctgcaataa acaaacgcag
    31081 gccagaaagc gccagttctg gcgaaccaac gccaaccacg cagaccagcc ggtgctctcc
    31141 tgctggcggc ttgccccttg tggctgaatc gtcggacgga tgagaaagcc gagcagcatg
    31201 gatgccacgc ccaacgtcaa cagcgcgagg atcacccgat aatcaaacat actgaccagt
    31261 ttgcccgtca gcgccgagcc aatgacaaac gccaccgagc cccacagtct cactttgccg
    31321 taatcaagcg ggaactgctt ttgccacgta ttcgccagtg catcggtcaa cggtaccagc
    31381 ggtgagaaaa agaggttaaa gccaatcatc accagcatca gccacgctac gtgcgccccc
    31441 gcccagaagg cgacagcaaa gagaagtgtc agcagtgcca gcacgcgcaa ggcggaaatc
    31501 aggcgggaag gatcgctgac gcggggcgcg atgagcaaac tcccgaggaa acgggcaacc
    31561 agacccgccc ccaataacag gccgatggtt tctggcgtta aaccaatccc tttaagccag
    31621 acgctccaga aaggtagaaa aatgccgtaa ctaaaaaagt atgtgaaata gccgagcgcc
    31681 aaccagcgcg tggattgcaa aaccatgagt ccctcccgtc aggaggcgtt agtctggggt
    31741 taatcgaatg ttggtgcaag ttgaaaaata agaatattcg agcctgcttc ctgacggtta
    31801 tcctgcatat caggaaatga caaaaaagag cggcccacaa cacaacatgt aaaaaagaac
    31861 gatgaaacag gtgaaaatcg caatttattg aactgacaaa ctcatgtgag acggataagg
    31921 cgtgcatgtc gcatccggca ggaaacaata agcacattgt cagccatctg cgttatgccg
    31981 ttataccaga aacgaatatc tcaacgcctg ggcgtggcga ggtaccagag tgtgtattac
    32041 gtttaaatca catcatcttg caaagggatt ggttatgaac tcactacgtt attttgattt
    32101 tggcactgcc cgccccgttt tattattaat tgcccgtatc gccgtggtct taattttcat
    32161 tatttttggt tttcccaaaa tgatgggctt tgacggtacg gtccaatata tggcctcgct
    32221 gggcgcgcca atgccgatgc tggcagcgat tattgcggta gttatggaag tgcccgccgc
    32281 gatattaatc atacttggct ttttcacccg tccgctggcg gtgctgttta ttttctacac
    32341 gctgggaact gcggtgattg gtcatcatta ctgggatatg actggcgatg cggttgggcc
    32401 aaatatgatt aatttctgga agaatatcag cattgctggc gcattcctgc tgctggcaat
    32461 taccgggccg ggggcaattt ctctcgatcg gcgttaggaa agatgccgga tgcggcgtaa
    32521 acgccttatc cggcctaccg ctccaacacc gattgtaggc ctgataagac gcagcaagcg
    32581 tcgcatcagg catcgtgcac cgagtgctac aaaatcgaaa ttaccccgta ggcctgtgca
    32641 tccggcattt tataaattac agcaacgcaa cccgcaacga catctctgaa attgttgatt
    32701 cccctggtgc aagcgcaatg aggtcaccac cttccggacg atgatggtca tccggcgcat
    32761 ggctcatcgg ttccagacag aagaaatcaa acgcatatcc tttatcaaac gcagggtctg
    32821 aaacaaagat gaaataacac tgtgcaggtg gcgtcgtttc catgatgatg gcataaccct
    32881 cctgcggctg ctcgatgcgg gccttaccat tccatccggc aaaaccattg tttacccact
    32941 ggcgcggcaa cggcgcgagc tggttaaaat ccagctcctg cggtagctgc tcgcaaaact
    33001 cacccgccag ccactgctct cgctccagcc agtaaccgct cgcctgtgcc tgaatccgcg
    33061 tttgcggcga caacggaaaa taaggatgcc agccggtacc aaatggcagc gtctctgccc
    33121 cttgattggt gacagcgagc gtcaccgtca gcgtatccgc cgttaaatga aatgcctggc
    33181 ttacccgata gtgataaaca ccgctgagat gttcatacac cagacacaag ctatcatcgc
    33241 tgcgcgagac acattgccac tcgcccaacc agccatcgcc gtgtagatag tgcgcatccc
    33301 actcaacatt cggttgcagt tgatactcac gcccctgcca gacaaaacgg ttgccgctca
    33361 cccggtttgc aaatggtacc agcggaaaac aagaggcatc ggttgccaca ccgctttttt
    33421 taccaggacg tagcagcggc gtcgtatcgc gccagaagcc ttcgattacg ccgccctgat
    33481 cagaaacgtc cagctttagc ggcccgtgag atagggtata gatggtcatc ctctactcct
    33541 taatccggga agttgatcac gactttcccg catttgccgc tcgccattag cgcgtaagca
    33601 tctcccgcct gctccagtga gaagcgatgg gtaatagcgt tacgcggcca cagtttccag
    33661 tcagtcaaat cgtgggcgca tttttccata tggaacaagc tggtcaccca ggagccaata
    33721 attctgcgtt ggtgatgcat caggtcagcg ctgacctcaa attccacttt tccggtttcg
    33781 ccaatgtaaa ccactcgccc ccagtcggcg gtagattgca atgccagcaa gcgccccgca
    33841 gcattaccgg aacaatcaag cgcaacatcc gcaccgccat gagtgagttc agcgatgatc
    33901 tgcggcagac cttcggtggt cgctaaatag ccgtgatcca tcacccctag ctgtttcgcc
    33961 atcgccagac gttccggcaa catatcaacg ccgatgatcc tttttgcacc acgacctttc
    34021 gccagcatca tcgccatcat gccgaccggc cccagaccga ccaccagtac gttatcactg
    34081 ccggaaactt cgccgcgcaa aatcccttca tacgctgtac caacgccgca actaataaac
    34141 gcaccatctt cgtaactcag tgcatccggc aggaggatca gatctttttc ttccgccagc
    34201 aggtattcag catggccgcc gtcacgctgc cagccgtaag ccgcttttcc ttcgccagta
    34261 caagagatag gaaaaccacg acggcagttc gggcaaaaac cacagccaga aatatgatac
    34321 accagcacac ggtcgccctc tttaaaatgg cggcagcctt gccccatcgc cacaatctgc
    34381 ccgcacggtt catgaccgtt gataaatccc tggtataacg gtttatcggg tgccgccgcc
    34441 gtagcacggt gctggtggta gatatagtgg acatcgcttc cgcaaatccc ggaggatttc
    34501 attttgatca gtacctggtt aatccccggc gtcggcaccg caacttcccg cagatcgacg
    34561 gtcgaatttc ctggtaaata agctgccagc atcgttttca taaatccctc attaacaata
    34621 cgattaattt tcatccctgc cgcacccgcg ccaggggcgt taaggtttag cgtttcgctt
    34681 tactgctacg ctgggtcagc aaggtattcg ccagcaccgc caccacgatg atgacgccgc
    34741 gcaccacctg ctggaaaaag gagttaatcc cgagcagcac cagaccgtta ccgattagcg
    34801 taatcaccag tacgccaagc agcgtaccga acaacgagcc gcgaccaccg gaaagcgccg
    34861 taccgccgac aacgaccgcg gcgatgacgt caaactccag accgtttgcg gcacctgcgt
    34921 taccggaacc gaggcgcgcc gccaacaaaa tgccggtcac cgccgctaat aatcccgaaa
    34981 gggtaaaaat aagaatgcgt acccgacgaa cgttaatacc gcacaactgc gctgcagtgg
    35041 cattaccgcc aacagcaaaa accgagcgcc cgaaggcggt tttgcggcta atgaacacaa
    35101 acagcgcaaa taacaccatc atgatcagcg cggataccgg caccccgaga aattgtccgc
    35161 ccagccagtc cagcacttcg ttttcgttaa ttggcactgg caaggcgttc gtcataaaca
    35221 gccccattcc gcgcagggcg ctccataaac ccagcgtcgc aacaaaactt ggcacgttaa
    35281 acacgccgcg cagcaccccg gcgagcgttc ccatcagcgc acctaacagc aacaccagca
    35341 gacaagcaat cgccagcgga acgtcaaatt gcagcaaaaa cgccaggcac actgaaacaa
    35401 aagccaccat cggcccaacg ctgacatcaa tttcaccgga gataataatc agcgtcatcg
    35461 cccaggcggc gatcccaatg gtggcggcat cgcgcagcac gttcatctgg ttattcaatg
    35521 agataaagcc aggcgcattc agggagaaga ccagataaag aatggcaatc accaccagca
    35581 aaccgatctc attaatatgg cgactgacaa attgtttgag cgagacgctc ttgccctgcg
    35641 gcaatggtaa tgacgaagca gacatcttca ggttcctcgg taaaattcat cagtgcacag
    35701 acagaatggc ggacatcagc tcatccacat tgaccggaga gtgaaactcc tgcgagaacg
    35761 taccgtgctg taacaacaga atgcggtcgc acaccaacgg taactcctcc acttcactgg
    35821 agataaacac cacgcttttt ccttcggcag ccagctcgcg gacaatacgg taaatctgct
    35881 gtttggcttc gatatcgacg ccgcgcgttg gctcgtcgag caacaaaatc tggctggcgg
    35941 catagaccca acgaccgatc accacttttt gctgattgcc gccagaaagc gtgccgatgg
    36001 gcgtttcgct actggcggcc ttgaccgtca tccgctgcat cacctcttcg gtcaggcggc
    36061 ggatggtgga ccattgcagc acaccgttgg cgctgatttt ttgccgattg gtcagcactg
    36121 tattttcgtc aacgcccaac cagggaatga tccccgcttc tttgcggttt tctggcgtat
    36181 agccaatgcc gcgtttcagc atgtcgccgt aatcggggcg cgtgattttc tcgccgttga
    36241 taacaatttc gccctgttca tacgtctcca gcccaacaat cgccttcagc aattcactgc
    36301 gccctgcccc cagcagacca gcaatgccga gcacttcgcc acgacgtagc gtaaaactga
    36361 tatcctccag cttgggctta tggcgtaacg cacggacttc cagcacggct tgatccataa
    36421 tttcctgagg ggcaaccggc gcaatatcaa cgtgatcgcg cccgagcatt agcgacacaa
    36481 tatgatgcgt agaggtgttt tcgagcatca catcgcccgc cacctgacca tcgcgcataa
    36541 cggtggcaca ggaggcaatg cggcgaattt cttccatccg gtggctgaca taaatcaccg
    36601 ccacgcccag tgccgacatc tttttcaccg cgctgatcac cagttcaact tccgcactgg
    36661 caagcgagct ggtgggttca tcaagaatga ccacgcgcgg ctcgcccttc atcacccgcg
    36721 caatttccac cagttgcttt tgcgccgggc ttagtgttga aacaagttgt tcaggactaa
    36781 cgtcaacgcc caacgcctgc aagcaacgtt gggcgtcctg cgccatttgc aggtaatcaa
    36841 tcatgccgtt gcggcggggc cactgaccga ggcagaggtt ttccgccact gtcagccctt
    36901 ccaccagact taactcctga taaaccgcac gaacccccag ttcagcggca cggcgagtca
    36961 gcgtagcttc gtcaccttcc agtcgcgtct cgccaatcca gatatcaccg ctatccggac
    37021 gttcgctacc ggtcagcatt cgaatgaggg tcgattttcc cgcaccgttt ttgcctaaca
    37081 gcgcgcgaac ttcgccttta ttgagcgtga agttaacgtt atccagcgca acgacgccgg
    37141 ggtaacgctt atttcctgcc accacttttg ctaccgggac tgcctctgtt gccgtgaaca
    37201 tagggacctc tgcgaatcag cgattagggc agaccatcaa cgtgcgttgc cagccactgt
    37261 ttaccgtctt ccgttttggt atagagatcg attggcacct gaatcacttt ttcaccgtcg
    37321 gcttgtttat tgataacctt caatgtttgc gcgaaaacag cattgcccat tttcttaccg
    37381 gaaatatcca ccaccgcttt cagcacctgg ttgttttcca gctcctgagc aatttcagtg
    37441 gtcatatcag aaccgaaaac agcaattttt ccggcctgat tttgattacg taccgctttt
    37501 accgcgccga gtgtcgctcc gcccgattcc cccataatgg cgttgagatc cggcgtggag
    37561 ataatcagtt tttcaccgac agaaatcgct ttatctaaaa cagtcccttc ctgattagcg
    37621 acaatttgcg cgccgggaac gcgggttttt aatacttctt caaacccttt acgacgttgc
    37681 acacaaactt caaaggcttc gcaattgatg acggcaattt tcggctggtc aattttgttg
    37741 gcaataaaat aatcggcggc tgcgttaccc agttttttac caaattccag cgggtcacca
    37801 accagatatg ccgagacata tttatcgaca cccttttgat taatacaggt gttgtagcaa
    37861 atcaccggaa tacccgcttc actggcgcga cgaacggtac ggctactgcc attttcagac
    37921 actgccgata aaataatggc atcgacatta cgcgccacga gggtatcaac aaaggtactt
    37981 tctttcgaaa tatcgccctg agcgttagtt tcaattaact gtacctgtac tgaagaatct
    38041 tttgccgcat cctgaacgcc ctgacgcact ccggcgtaat atccctgggt atcaaggtat
    38101 attgcgccaa tagtcatttc tttatcagaa gccctggcaa atagtgcgct acccaatagc
    38161 gtagccatta ataataaatt acgggttctt gtcatttttt taggcatagt gcctcctgta
    38221 ggttttttat ttacaacggc ttattttaat tatttgagcg tgagcggcag cgcgtgccgc
    38281 tcaaatatta cagttaatag ggtttagtga acatagctaa aaataaaagg catcacggta
    38341 gtgaataatg ccgctttcgg ccaggctgga ttaatcgcca tcaattcagc gcgagcagat
    38401 tcatagtgcg caacctcccc taatcccgcg gccgccagca tcgccaccat caggcatttt
    38461 tctttatgct gctgttgtaa atcgccatac agcgacaaca agtcaggctg cgagacggca
    38521 aagaaatccg cctcgatact ggttttcgcc atctctttcg cccactgttt catttcacta
    38581 aacagttgct gtgcggtgtg ttgttcgccc agcagtcgca gcgccattcc ttgccagaag
    38641 agataatcaa ccggctgatc gttgtaataa ctgtggatgt taatggtgcg atcgccggtc
    38701 gccgccagac gtaaacaacg cgtcgcttca gtttcatcgc cctgcgcgtt ggcgcatact
    38761 gcctgccaga accagatgtc gttgtcagtt tgccccggta aacggccttc gcttaaattc
    38821 tccggataat gcagcgcggc atgaagcagt tcgctggcct gctgcggctc tctgtcatca
    38881 agatgctgcc aggcgcgtaa taactggttg aggataaact ggctggtgac cttcccttcc
    38941 ccaccttccc acgggtggaa tttgcgcgtg gcgagaatgt ccgccgcttt gtctgcctga
    39001 ccagtgagat gccacaaatt gagcagttct gcggtcatgt cgtcgcgttt cagcgcaatt
    39061 tccagattat tttccagacg cgccagtcgt ttctccggtg tgacaccgct taacttatcc
    39121 agcaaatccc gttcgaaaag cagacgtgca tcctgcggcg caagctgata agcattatca
    39181 agataacgcg cggccagctc ataatcgtgc tgcttattcc acgcatggat cgccaacccg
    39241 cgccagccgt cggcaaactc cggcgacatc tctacgcaac gctgccataa ggcaatggct
    39301 ttaccgtagc tacgcttgtt gtagtagaag caggccagta aatgccgggc aaaccagcac
    39361 tcttcgatac tctccagcgc cgccacttct tccagcgtat tcgggaaacg gacaaactgc
    39421 gggaaggcat caatagcttt tacgaccagt tctccgcgtt cagcttttgg tagcaaactg
    39481 gcttgcaggt aaagcggtag cgtgcgctga cagtccagtg cgttcagcat atctgccgcc
    39541 agggcgggca tgccccagtt aatcagttgc ccggcggtta tcagggcgtt aacgtcgcgt
    39601 ccctgacaaa gacctcgcca ctgcgcgagg gcagattcgc tacgaccatc gaaccagttc
    39661 agccaccaca gagtagcgtt cagcggataa tcacgcagca gtttctcgcg ctgcaaacgc
    39721 gcgttgtcct gacgaccact taacaccagt agcaggttat gcaggcaaag cacttcctga
    39781 ttggttgggc aggcgcgaag actttgttgg caaaaatcca gaccagcgtc gaagtgacca
    39841 ttacgcgccg ccagtcgtgc cagaccataa tagccaccgg ctttactgtt gccgctccag
    39901 accgcacgcc agaaatcctc ttcggcttgt tgatattgtc cctgacgttc gtaagcactg
    39961 gcgcgaatca aactcgcctg tccgcactgc ggatttttgt tcagcgcatg tgcgcgtttc
    40021 agcgcctgag tggcatacgc caccgcttgc gggaaatcgg cacggttata ttccagcatt
    40081 gccagcgcca gattacagcg ataatccagc ggatccagcg ccacgccgcg caggtagtaa
    40141 tcgaacggtg aacggctcgc gtgatgatat tgctccagat gttgaccgat aaaccaggct
    40201 tcatctgtac tggtaatgtc ttgtgctgcc agtggcgctt tggcgacgtc cggcaacggc
    40261 aacgcttgcg gctgatgttc atgataactc agtacaatat tgccgtcggc atcggagagc
    40321 tcaatggtca gcctttcagg attgataccg tgcaacacgc cctggatggc ggtcgctggt
    40381 gtcagggcca cggcatcatc gagcaacgcg ttgcatttgc cgatttcgcg gatcgccagg
    40441 cggtatccgt tcaacggaga gatggcatac agcccccact caatcccccg ctcactacgc
    40501 tggagtttta tcaccgcatc gcgggaggca ttttgcacca tgcccagaga atgataaggc
    40561 aggaaatact gctcgaaacg cttctcctcg taagcatcaa gccaggtaaa atcaggctgg
    40621 ttatcggcaa aaataccggt catcagttcg atatacgggc cgttattgtc agtcaggctc
    40681 ttatcccacg cctggccaaa ttcactgtgt ccccagctcc actgtttttt acctggcgca
    40741 atatggtggt tggcaacgtg tagcaaacca ccatcttcat cgtgacacca cgcgccaaca
    40801 aaatcgtact gtgatttttc agccatatat gaggttggaa cgggcacatt tttatagcga
    40861 gaaatgtcca ctccggcgga gtagtccact ttgtagtaag tgccggtggc gatggggaaa
    40921 gcggagacgg cccgtttacc gtgatcaaac accgcagtca catccggtgg gaaaacgctc
    40981 tgatgccctt cacccccttt cactgccggg ttggcccacc acaagaaatg acgcggcgtg
    41041 gcgttgccgt tatagacgcg actggcgatt tccagcgccg cccggtcagg gcgcagggtg
    41101 aaacctgtca tcacctgtaa accgtgcatc ggctccgttt cgccgaccca caccgtctgt
    41161 gcgccgtcgt catgggcttc gagggtgaaa tcaacgggca taaaggtggt cgggcgatgg
    41221 tgttgcggcc agttaaactc aatcccgccg gagatccacg gccccagcag tcccaccagc
    41281 gccggtttaa tgacttcatt gtgataaaca aaatcgcgct gtttcacttt atcccacgcg
    41341 cgatgcaccc gaccgcccag ttccggcagg atcatcactt tgatgtagtc gttttccagc
    41401 cacaccgcct gccaggattt cagggttttc tgctcgctca gcgtatcggt cacgccgtag
    41461 ggataaaccg cgccggacga tccctgataa acgcgatttt ccaggaacat gggatgtata
    41521 tcctgcggcc cggtttcata ggtcgggatc tcaacgcgct cttgccacac ttttactgga
    41581 gtcatggtgc cctcaatatt aacaagacat actgaattaa aagatttgtg gcagtgtatt
    41641 gaacaatctg gcactgtttt cgcggaataa tcacgcaatt aactaaacaa ggtttagtaa
    41701 agatgagagc ctgcattaac aatcaacaga ttcgccacca taacaaatgc gtgattctgg
    41761 aactgctgta ccggcaaaag cgcgccaata aatcaacgct ggcccggctg gcgcaaattt
    41821 cgattccggc ggtcagtaat attttgcagg aactggaaag cgaaaaacgg gtggtgaata
    41881 tcgacgatga aagccagacg cgcgggcata gtagcggtac atggctgatt gcgccggaag
    41941 gagactggac gctgtgcctg aacgtgacgc ccaccagcat tgagtgtcag gtcgctaatg
    42001 cctgtttaag tccgaaaggc gaatttgagt atttacagat tgatgcaccg acgccgcagg
    42061 cgctgctgtc cgaaatcgaa aaatgctggc atcgccaccg taaattgtgg ccggaccgca
    42121 ccatcaatct ggctctggca atccacggtc aggttgatcc agtgaccggc gtgtcgcaaa
    42181 ccatgccgca agcgccgtgg gcaacaccga ttgaggtgaa gtacctgctg gaagagaagc
    42241 tgggcattcg ggtaatggtc gataatgact gcgtgatgct ggcgctggcg gagaaatggc
    42301 aaaataattc gcaggaacgg gatttctgcg tgatcaacgt tgattacggc attggctcgt
    42361 cgttcgtgat taacgagcaa atttatcgcg gcagtttgta tggtagcggg caaattggtc
    42421 acaccatcgt taatccggat ggcgtcgtct gcgactgcgg gcgttatggc tgcctggaaa
    42481 ccgtcgcctc gttaagcgca ttaaaaaaac aggcgcgggt atggctaaaa tcacaaccgg
    42541 ttaatactca gcttgatcct gaaaaattga ctaccgcgca gttaatcgct gcctggcaga
    42601 gtggagaacc gtggatcacc agttgggttg accacagtgc caacgccatt ggtttgagtc
    42661 tgtataactt cctcaacatc ctcaatatta atcagatttg gttgtacggt cgcagttgtg
    42721 cctttggtga gaactggctt aataccatta ttcgccagac gggatttaac ccgttcgacc
    42781 gcgacgaagg accgagcgtg aaagcgacac agattggctt tgggcaatta agccgcgcac
    42841 aacaggtact gggaattggc tatttgtatg ttgaggcgca gttacggcag atttgatggc
    42901 gcgataacgt agaaaggctt cccaaaggaa gccttgatga tcacaaacct aagattgcct
    42961 gatgcgctac gcttatcagg cctacacgga tattgcaata tattgaattt gcaaagtttt
    43021 gtaggccgga taaggcgttc acgccgcatc cggcatgaac aacgagcaca ttgtcagcaa
    43081 atcaccgttt cgcttatgcg taaaccgggt aacgtgcgca gatgtcgaga actttacctt
    43141 tgatgcgctc gataacggct tcatcattga tgctgtccag cacgtcacac atccagccag
    43201 ccagttcttt cgcttcggct tctttaaagc cacgacgggt aatcgccgga gtacctacac
    43261 gaataccgga agtcacaaac gggctcttcg gatcgttcgg tacgctgttt ttgttcacgg
    43321 tgatgttagc acggcccaga gcggcgtctg cttctttacc ggtcaggttt ttatcaacca
    43381 gatcaaccag gaacaggtgg ttatcagtac caccagaaac cactttgtag ccgcgttcga
    43441 ggaacacttc taccatcgct ttggcgtttt tcgcgacctg ctgctggtaa gttttgaact
    43501 caggttccat tgcttctttc agagcaaccg ctttaccggc gattacgtgc atcaacggcc
    43561 ccccctgacc accagggaaa acggcagagt tcagtttttt gtacagctct tcgctaccac
    43621 ctttcgccag gatcaggccg ccgcgcggac ccgccagggt tttgtgagtg gtggtagtaa
    43681 caacgtgagc atgaggaacc gggttagggt agacaccagc ggcaaccagg cccgcaacgt
    43741 gcgccatatc aacgaacagg taagcaccga tgctgtcagc gatttcacgc attttcgccc
    43801 agtcaaccac gccggaatat gcagagaagc caccgataat cattttcggt ttgtgttctt
    43861 tggcttgttt ttccagatcg gcgtagtcga tatgaccggt agcatcgata ccgtaaggaa
    43921 cgatgttgta cagtttaccg gagaagttaa ccggagaacc gtgagtcagg tgaccgccat
    43981 gcgccagatt catacccaga acggtatcgc ctggttccag caacgcggtg tagaccgcaa
    44041 agttagcctg ggagccggag tgcggctgga cgttagcgta gtcagcgccg aacagttctt
    44101 tcgcacgatc gatcgccagt tgttcaacga tatcaacata ctcgcaaccg ccgtagtagc
    44161 gtttgcccgg ataaccttca gcatatttgt tggtcagctg agaaccctgc gcctgcatta
    44221 cgcgcgggct ggtgtagttt tcggaggcga tcagttcgat gtgctcttcc tgacgtactt
    44281 tttcctgctc catagcctgc cacagttcgg catcataatc ggcaatgttc atttcacgct
    44341 ttaacatccg catctcctga ctcagctaac aataaaattt ttggccttta taggcggtcc
    44401 tgttggacaa cggcgaacag tataaccgaa tcattgtgcg ataacaggtc ttgacaaagg
    44461 aatttacgca aacgattacc ttcaggctac gcaaggcttt ggagaataaa gagcttgcaa
    44521 ccggaaacgg atttcttttc aggtttgtga tgcaaatttt tcacttcatc acattctttc
    44581 tgaaaaacac caaagaacca tttacattgc agggctattt tttataagat gcattttaga
    44641 tacatcaatt aagatgcaaa aaaaaggaag accatatgct tgacgctcaa accatcgcta
    44701 cagtaaaagc caccatccct ttactggtgg aaacgggacc aaagttaacc gcccatttct
    44761 acgaccgtat gtttactcat aacccagaac tcaaagaaat ttttaacatg agtaaccagc
    44821 gtaatggcga tcaacgtgaa gccctgttta acgctattgc cgcctacgcc agcaatattg
    44881 aaaacctgcc tgcgctgctg ccagcggtag aaaaaatcgc gcagaagcac accagtttcc
    44941 agatcaaacc ggaacagtac aacatcgtcg gtgaacacct gttggcaacg ctggacgaaa
    45001 tgttcagccc ggggcaggaa gtgctggacg cgtggggtaa agcctatggc gtcctggcta
    45061 atgtatttat caatcgcgag gcggaaatct ataacgaaaa cgccagcaaa gccggtggtt
    45121 gggaaggtac tcgcgatttc cgcattgtgg ctaaaacacc acgcagcgca cttatcacca
    45181 gcttcgaact ggagccggtc gacggtggcg cagtggcaga ataccgtccg gggcaatatc
    45241 tcggcgtctg gctgaagccg gaaggtttcc cgcatcagga aattcgtcag tactctttga
    45301 ctcgcaaacc ggatggcaaa ggctatcgta ttgcggtgaa acgcgaagag ggtgggcagg
    45361 tatccaactg gctgcacaat cacgccaatg ttggcgatgt cgtgaaactg gtcgctccgg
    45421 caggtgattt ctttatggct gtcgcagatg acacaccagt gacgttaatc tctgccggtg
    45481 tcggtcaaac gccaatgctg gcaatgctcg acacgctggc aaaagcaggc cacacagcac
    45541 aagtgaactg gttccatgcg gcagaaaatg gcgatgttca cgcctttgcc gatgaagtta
    45601 aggaactggg tcaatcactg ccacgcttta ccgcacacac ctggtatcgt cagccgaatg
    45661 aagccgatcg cgctaaaggt cagtttgata gcgaaggtct gatggatttg agcaaactgg
    45721 aaggtgcgtt cagcgatccg acaatgcagt tctatctctg cggcccggtt ggcttcatgc
    45781 agtttgccgc gaaacagtta gtggatctgg gcgtgaaaca ggaaaacatt cattacgaat
    45841 gctttggccc gcataaggtg ctgtgatttg atgttgccgg atggaaacat ccggcaaccc
    45901 ttgacgcgtt ttaaattgcc gcgtcgtcct cttcaccggt acggatgcga atgacccgtg
    45961 ccacgtcgaa gacgaagatt ttaccgtcac cgattttgcc ggtttgcgcc gtgcgaataa
    46021 tggtatcgac acaggtatcg acaatgtcgt ccggtacgac aatctcaatt ttcaccttcg
    46081 gcagaaaatc caccatatac tccgcgccgc ggtacagctc ggtatggcct ttttggcggc
    46141 caaaaccttt cacctcagtg accgtcatgc cggtaatgcc gacttcagcc agtgcttcgc
    46201 ggacatcgtc cagcttgaag ggtttaataa tcgcatcaat ctttttcatg ctattccttg
    46261 aaaaggtcgc ctgtcttttg atctgctaaa cgtaacacat aacgccaatt cattccttga
    46321 aatcgtttgc atccagctcg tgtcgggaaa gcagtttata aaattctgtc cggttgcgcc
    46381 ccgccattct cgccgcgtgg gtgacgttgc ctttggtgat ttgcagtagc ttacgcaaat
    46441 agttgagttc aaactgatta cgtgcctcaa caaaggttgg cagcgccgta ttttcaccct
    46501 ccagcgcctg ctccaccagc gcatcactaa tcaccggaga tgaggtcagc gccacgcact
    46561 gttcaatcac gttgaccaac tggcgcacat tacccggcca gctcgcggtc atcaggcgtt
    46621 tcatcgcatc ggtggagaac gcgcggacaa acggtttatg tcgctctgcc gcctggcgca
    46681 acaggtgatt tgccagtagc ggaatgtctt ctgtgcgctc tgccagtgcc ggaattttca
    46741 ggctgacaac gttgaggcgg taatagagat cttcgcggaa ttccccgcgc gccatcgctt
    46801 ttggcaggtc gcggtgagtg gcagaaataa tccgcacgtc gatatcaata tcgcggttac
    46861 tgcccagcgg gcgcacttta cgctcctgca acacgcgcag cagtttgacc tgcaacggtg
    46921 cgggcatatc gccaatctca tcgagaaata gcgtaccgcc ttccgccgcc tggaataaac
    46981 cttcgcgatt gctgacagcg ccagtaaacg cgccacgcgc atgaccaaac agctccgact
    47041 ccagcaattg ctcgggcaat gcgccacagt taatagcaat aaatggtttg ctgttgcgcg
    47101 ggctggcgtt gtggatagcc tgggcgaaaa tctctttccc ggtgccgctc tggccgttaa
    47161 tcaaaacgct aacgtctgat tgcgccacca gccgcgcctg ttccagcaaa cgcagcatca
    47221 gcgggctgcg ggtgacaatc gcctcgcgcc agcgttcgtc ggtggctggc gcggattgtt
    47281 ccagcgcatc gtcaattgcc tgatatagcg catctttatc gacaggcttg gtgagaaaac
    47341 tgaacacgcc ctgctgtgtt gcagcaacgg catcaggaat agaaccatgc gcggtaagaa
    47401 taattactgg cattcccggc tgcactttct ggatttcagc aaacagctgc ataccgtcca
    47461 tttcatccat ccgcaggtcg ctgatgacta aatctacttt ttcgcgattc agtacccgca
    47521 atccttcagc accactttcc gccgtgacca cactgtagcc ttcgctggtc aggcgcaggc
    47581 caagcagttt cagcaatccc ggatcgtcat cgaccagtaa taaatgcgca ggtttatggc
    47641 tcatcaggag tgacctcatc ggtggatggc gcgggctttt cactctcgtg cggcgtatcc
    47701 ggcgagaaat ttccggccgg tttgcgggtc gagagctggc gttcaatatc ggtcaggttt
    47761 tccagcttgc gggtagtgag ttccagttgc tgttgtagaa cgtgatgttg ctggcgcaaa
    47821 gtatccagct cgctgtcgct ggactgctgg agtttgctgt agcgttggcg ctcttccgcc
    47881 agttgcagtt gcagcgcctg accgtcgcgc caaagttgat acagtgggcg aacctgtgcc
    47941 gggatctcgg tacttagcgc atcaatgcgc gcgaccagct ggcggcgctc atacggagta
    48001 attttggcgt cggcgagcaa aatcccttgt ttaaaggtat tttgccagtt gccgtcgtca
    48061 tattggcggg cttgctgacg cgactgcgca ggcattaaac gatcagcaca atccatcgcc
    48121 cgcagccagt aaagcggatt ggtttcggtt gatttacctt gcagcgccca gatgtcgcta
    48181 cattcagtag aaagatagtc agccagttga taaaccggaa ttttttcttc tgctggcgta
    48241 tcaatggctg gcctattgtg actctgcacg caacccagca atgccagaca tggcagccct
    48301 gccagccaca gccgtcgggg caataatcgt tgaaaaatgt gtcgcatatt caccagactt
    48361 aaagcctatc ccagtgggcg taattgttgc aggcagtttg gacatggaca gcgcggagaa
    48421 accggagcgt acatgtcgta cgtgaagatt tcgagcactg cccggggccg aactgacaaa
    48481 taaaatagcc tgatgggatg ggctgttagc attatttcgt gtttttcgac gacggtaatt
    48541 caatgcggaa acaaacgtct tgcccgctct cgtcgaccag atacagttcc ccttgcatac
    48601 ggcgaataca atccctggca atgcttaatc ccagaccgct gcccttcacc gccccttttc
    48661 gctggtggct tccctgaaaa aagggttcga agatcatggc gcgttcctct tgcggaatgg
    48721 gcgtgcctgt attgatgaca tcaatataaa cccgcgcacc atgtaaactg ctgcgaaggc
    48781 aaatgttacc ggattcagcc ccgtagtgca ccgcattgga gtaaagatta tccagtacgc
    48841 tcatcagcag cattggctcc gccaggcaag ctgttgcttt gagatcaacg tcggtatgca
    48901 tcattttagc ccgtgcgggc aggctatgag cagaaaccac tgtctccacc agcggtgcta
    48961 actcaacatt ctccagttcc accgcactgt ccgcctgttt acggttgtaa tcaagcagtt
    49021 gttcgatcag cttttgcaaa ttgcggctgc tgctatcaag aatgctcacc acctcttttt
    49081 gctctggcgt aagcggcccg acaacctgat cagccagtaa ttcagtgccc tcgcgcatac
    49141 tcgccagtgg cgtttttaat tcatgagata aatgtcttaa aaattgatgg cgttgggatt
    49201 ccagccatga caggcgctca cttaaccaaa gaatacgttg cccaaccgag cgtaactcgc
    49261 tcggtccact gaacgagacg ctattgccca gagaacgccc ttcccccagc cggttgatca
    49321 tgcgctcgat atttttcacc ggaccgataa tcatcctcgt gaaaagcagt accatcacca
    49381 gactcaccag aaatagcacc agcgattgcc aaccaaaata ttgcccacgt tcggcgattt
    49441 cacgctgaag ttgctgccca cgagagaaca ccactgtgcg cgtggcctgc accatttcgg
    49501 tattggcact ggcaaaggct tccagacgcg cggcggcagc agcatcggga ccgctgttgt
    49561 tacactgaag ttgagccaga ttgttcaaat cctgacgtaa tgcctggtag agtttatcgt
    49621 ccggcagcac gcctgcgtgg gcatcgagca tttcgctgta acgcttgcgc tggctttgat
    49681 aaaccttcgc aagcgtaggg tcgtccagca cgcagtactg acggtagctg cgctccatct
    49741 ccagtgccgc gttggtcatc gcttcactgc gccgggcatc aataagcgta gtgcggttaa
    49801 ccagcgccgc ctgatcgcta agtgcattca ggctttgcca ggcttgccat gccagcacca
    49861 acaggggcag cagaatcagc aaaaatgcca gcattaccag ttgtcgtaat gagcggggaa
    49921 aaacgggcca gcgtttcaag gcgttactct cgtcagacgc gaatagcctg atgctaaccg
    49981 aggggaagtt cagatacaac aaagccggga attacccggc tttgttatgg aataaggcgg
    50041 tgcctaactc gacgtttcgc ccgatggttg atatagctac gctgatatca gaagttggac
    50101 ggcaggcacc ttgttgtgcg tcattcgaat tttatgtagc acgtcccgaa ggggctgaca
    50161 taagtcggtg aatgagccac tgcttactat tatgcagtaa ctgtgccaat aaagaaaata
    50221 gtttggtaac atactgatta tacgctgctt tgaggggttt atggcttctc cgctatttga
    50281 atgtaaatca gccactgtgt cgctaaaaag agacaactta agataaactt attagataat
    50341 attaaaatca atgagttaag tgtcaccaaa aagcgacacg gcaaaccacc cattgtcgtg
    50401 atttaaagac acaaaaaagc tcccgaagtt gggagctcat aatagcggtt ggtgcttaat
    50461 gccggatgcg gcgtgaatgc ctcatccggg ctgcaatacc aatgggttga cgacttaccc
    50521 caactgctta cgtgcattgc ggaaaatacg catccacggc ccatcctcgc cccagttttc
    50581 cggatgccag gagttgctga cagtacggaa aacacgttcc gggtgcggca tcataatggt
    50641 gactcgacca ctttcagtcg tgactgccgt aataccattc ggagaaccgt tcgggttagc
    50701 cgggtaggtt tcagtgactt tgccgaagtt atcgacatag cgcagtgcca ccagcccttt
    50761 gctttccagt gccgccagat gcgccgcatc gcgcacttcc acgcgccctt caccatgaga
    50821 gacagcaatc ggcatctgcg agcccaccat cccctgcaac agcagcgacg ggctttgggt
    50881 tacttctacc agactgaaac gcgcttcaaa gcgatcggag gtgttacgca caaaacgtgg
    50941 ccacaactcg ctacccggga tcagttcacg cagattagac atcatctggc aaccgttaca
    51001 tacccccagc gccagcgttt gcggacgatg gaagaaggtt gcaaactcat cgcgtacacg
    51061 gtcattgaac aggatagact tcgcccagcc ttcaccagca cccagcacat caccgtagga
    51121 gaaaccgccg cacgctacca gagcgtggaa atcctccagg cccgtgcgtc cagccagcag
    51181 gtcgctcata tgcacgtcga tagcatcgaa gcccgcacgg tggaaagctg ccgccatttc
    51241 aacatgcgag ttcaccccct gctcacgcag cacggcaact ttcggacgcg caccagtggc
    51301 aatatacggt gctgccacat cttcgttgat atcaaacgac agtttcacat tcaggcccgg
    51361 atcggcgtcg tttgatttcg cctggtgctc ctggtcagca cactccgggt tgtcacgcag
    51421 acgctgcatc tgccaggtag tttctgccca ccagacacgc aacgtggtgc ggctttcgct
    51481 aaatacagtc tgcccgttgg cggtaatcac aaaacggtca ccgggaaccg cctgcccaac
    51541 ataatggacg caatcagcaa gcccatgctg tgccagtacc gcttcaacgg cttcacggtc
    51601 agcggctcga acctgaatca ccgcacccag ttcttcgcta aacaatgcag ccaggcgatc
    51661 gtcacccaga gtggcgatat ccgcgtcaat gccacaatga ccagcaaacg ccatttccgc
    51721 cagcgtcacc agcaggccac cgtcagagcg gtcgtggtac gcgagcagct tacgctgtgc
    51781 aaccagcgcc tgaatcgcgt catagaagcc tttcagttgc gcgacatcgc gtacatctgc
    51841 cggtttgtcg ccaagctgac gataaacctg cgccagcgcc gttgcgccca gcgcgttatt
    51901 gcctttgcct aaatcaatca gcagcagtgc gttatcttcg gtagaaagct gcggcgtgat
    51961 ggtgtgacgt acatcttcca cgcgggcaaa tgcagaaatc accagcgaaa gcggcgaggt
    52021 catttcgcgc tcttcgctac cttcctgcca gcgggttttc atcgacatag agtctttacc
    52081 caccggtatc gtcaggccca gcgccggaca aagctcttcg cccaccgctt taacggcttc
    52141 atacaggccc gcatcttcac cagggtggcc tgccgccgcc atccagttgg cggaaagttt
    52201 aatgcgtttg atatcgccaa tttgtgttgc ggcgatgttg gttagcgctt caccgactgc
    52261 cagacgggca gaagcggcga aatccagcag cgcaactggc gcacgttcgc caattgccat
    52321 cgcttcgcca tagtagctgt cgaggctggc ggtagtgacc gcgcagttag cgaccggcac
    52381 ctgccacggc cccaccatct gatcgcgcgc caccatgccg gttacgctgc ggtcaccgat
    52441 ggtcaccagg aaagttttct ccgccacggt cggcagatgc agcacgcgtt tcaccgcgtc
    52501 agcgatggtg atcccttcac gggccagcgc gtcgcctttc gctttcagcg tttgtacatc
    52561 gcgggtcatc ttcggcgttt tgccaagcag tacgtccagc ggtagatcaa tcggctgatt
    52621 atcaaaatga cgatcgtgca aagaaagatg cagttcttcg gtcgcttcac caatcaccgc
    52681 gtagggtgcg cgctcacgct tacacagttc gtcaaacagc ggtaactgat cggcagcaac
    52741 cgccagcacg tagcgttcct gggattcgtt acaccagatt tccagcgggc tcatgcccgg
    52801 ttcgtcgctc aggatatcgc gcagttcaaa tttaccgccg cgcccgccgt cgctcaccag
    52861 ttccggcatg gcgttagaaa gaccgcctgc gccaacgtcg tggataaaca ggattgggtt
    52921 ggcatcacca agctgccagc aacggtcgat cacttcctgg cagcgacgct ccatctccgg
    52981 gttgtcgcgc tgtacggaag caaagtcgag gtcggcatca gactgaccag atgccatgga
    53041 agacgccgcg ccgccgccaa gaccgatgtt cattgccggg ccgccgagaa cgaccagctt
    53101 cgcaccaacg ttgatctcgc ctttttgtac gtgatcggcg cgaatgttgc cgatcccacc
    53161 cgccagcatg atcggtttgt gataaccgcg cagctcttcg ccattgtggc tgttcacttt
    53221 ttcttcataa gtacggaagt agccgttcag tgccggacga ccaaattcgt tgttaaacgc
    53281 cgcaccgccc agcgggcctt cggtcatgat gtccagcgcg gtaacaatgc gctcaggttt
    53341 accgaaatct tcttcccacg gctgttcgaa gccaggaatt cgcaggttgg atacggagaa
    53401 accaaccaga ccggctttcg gctttgcgcc acgtccggtg gcaccttcat cgcggatttc
    53461 accgccggaa ccggtcgccg cgcccggcca cggagaaatc gccgtcgggt ggttgtgagt
    53521 ttcgactttc atcagaatat gcgccggttc ctgatggaaa tcgtagcggc ccgtttcgtg
    53581 gtcagcaaag tagcggccca cttcagaacc ttccattacg gcggcgttat ctttataagc
    53641 agagagaacg tgatccggcg tggtttcgaa ggtgtttttg atcatcttaa atagcgattt
    53701 cggctgctgt tcaccatcga taacccagtc ggcgttaaaa attttgtggc ggcagtgctc
    53761 ggagttcgcc tgggcaaaca tatacagttc gatgtcgttc gggttacgac caagctttgt
    53821 gaaagcatcc tgcaaatagt caatttcatc ttccgccagt gccaggccaa gacgcaggtt
    53881 agcgtcgatt agcgcctgac ggccctgccc cagcaaatca acgctggtga ccggagtcgg
    53941 ttgatggtgt gcaaacagct gctctgcatc atctaaagca aaaaagaccg tttccatcat
    54001 gcggtcgtgc agttcagcgg taacctgctg ccattgttca ttggtcagcg taccggcttc
    54061 tatatagtaa gcaacgccgc gctcaaggcg gtttacctgt tgcagcccgc agttatgggc
    54121 aatatcggtc gctttcgaag accagggaga gatggtgcca ggacgcgggg tcaccagcag
    54181 gagtttgcct tgcggggcgt ggctggcgag tgccgggcca tatttcagca ggcgttcaag
    54241 ttgtgcgtgc tcatcatcgt ttaacggcgc attgaggtca gcaaaatgga catactcggc
    54301 gtaaatagtg tgaaccggga gcctggcagc ctgaaaacgt gccagcagtt tgttgattcg
    54361 gaatgccgac aatgcaggcg aaccacgcag aatttccatc ataagtctct cgtcttctaa
    54421 gctttcggtg tacccaaggg ggggaaacgg gcgtcattat aaagaatctg atgcgctgac
    54481 gaaaccgttt gcgtggaaat aaaatcacca tcgtgaatta gcaacgcgtg ccgccaatgg
    54541 ctgtaataag ttgccatctg gcgcaggttt acgcaaaatg ccgctcattt atgagtaaac
    54601 ctttcactat tattacgttt tttcaagctg ggacgcgcac gacacagaga attaactaat
    54661 tgaaaaaatt aaagattaat tatctgttca tcggcattct ggcactgctg ctcgcggtcg
    54721 ctctctggcc atccattccc tggtttggta aagccgacaa ccgtatcgcc gccattcaag
    54781 cgcggggaga gttgcgtgtg agcaccattc atacccccct gacttataac gaaatcaacg
    54841 ggaaaccttt tggcctggat tacgaactgg cgaaacagtt tgccgattac ctcggcgtaa
    54901 aactgaaagt gaccgtgcgg cagaatatca gccagctgtt tgacgacctc gataatggta
    54961 acgccgacct gctggcggca ggcctggtct ataacagtga gcgggtaaaa aattatcagc
    55021 ctggccctac ctattattcc gtgtcacaac aactggttta taaagtcggt cagtatcgcc
    55081 cacgtacgct gggcaacctg acggcggagc aactcaccgt tgcaccgggt cacgtggtgg
    55141 ttaacgatct ccagaccctg aaagacacaa aattcccgga attaagctgg aaggtagacg
    55201 acaaaaaagg ctctgtggaa ttaatggaag atgtcatcga aggaaaactc gattacacca
    55261 ttgctgattc tgtcgccatc agcctgtttc agcgcgttca cccggaactc gccgtagcgc
    55321 tcgatatcac cgatgaacaa ccggtgacct ggtttagccc gttagatggc gataataccc
    55381 tttccgccgc cctgctcgac ttcttcaacg aaatgaatga agacggtaca ctggcacgca
    55441 ttgaagagaa atacctgggg catggcgatg attttgatta cgtcgatacg cgcacatttt
    55501 tacgcgccgt cgatgcggta ctgccgcagt taaagcccct gtttgagaaa tacgccgaag
    55561 aaattgactg gcgtttgctg gccgctattg cttatcagga atcgcactgg gatgctcagg
    55621 ccacttcacc gacgggtgtg cgcggcatga tgatgttaac caaaaacacc gcgcaaagcc
    55681 tcggaattac ggatcgtacc gatgccgaac agagcatcag cggcggcgtg cgttatttgc
    55741 aggatatgat gagtaaagtg ccggaaagtg tgccggagaa cgaacggatc tggtttgccc
    55801 tcgccgcgta caatatgggc tatgcgcata tgttggatgc ccgcgccctg acggcaaaaa
    55861 ccaaaggtaa tcctgacagt tgggctgacg taaaacagcg tctgccttta cttagccaga
    55921 aaccctatta cagcaagctg acttacggct acgctcgtgg gcatgaagcc tacgcttatg
    55981 tcgaaaatat tcgtaagtat cagattagcc tggtgggtta tctgcaggag aaagagaagc
    56041 aggctacaga agcggcgatg caactggcgc aggattatcc ggcggtatcg cctacggagt
    56101 tgggcaaaga gaaatttcct tttctctcgt ttctttccca gtcgtcatca aactatttga
    56161 cccactctcc ctctctgcta ttttccagaa aagggagtga agagaaacaa aattaatctg
    56221 tcgaggattg cgcttttttc tgcgctttaa tttcctggcg gcgcatgcga aagaagtcac
    56281 tgagcaacgc cgcgcactca tccgccagta ttccttccgt aatttccacc cggtgattca
    56341 tacccggatg atgtagcaca tccattaaag atcccgcagc gccagttttc gcgtcacgcg
    56401 caccaaagac cacgcgacca atgcgactgt ggatcatcgc tccggcacac attacacagg
    56461 gttcaagcgt gacatacaac gtggcgtcga tcagacgata attttgcatc accagaccgc
    56521 cctgccgtag ggccatgatt tctgcatgtg cggtgggatc atgtcgacca atcggacggt
    56581 tccagccttc gccgattacc cgattgttat gcactaatac cgcgccgacc ggtacttccc
    56641 gctcatccca ggcacgcttc gccagcgtca ttgcatgacg catccagtat tcgtggctaa
    56701 attcgacttc agacaaaaag aaaactccgg ttataaaagc gcggcgcatt atacacggac
    56761 gctatgcttt actctattcc agttgctgga gttcaccgcg cggggttacc cgccagcgat
    56821 gctggcaaaa gtaaagcagc gggttgtcct ggttactgtc gctatagcca ctgtacagcc
    56881 gcagcggcgt gccgattttg cgctccagtt gcgcgacctt ttcatgtccc agacaacgca
    56941 tcgtcaatac ccaaccacca tagccacgct gaatctgact ggcgataaga ttaacccgcg
    57001 gcagccaggg cgtatcgaaa taaaccgctt caaccagcgg ctgcggagag ccggtaatca
    57061 accagatatc agcatcggaa cttaacaggt aggtggttaa tcgctcctga accagcggaa
    57121 aggcggtaac attgtcacga aaccagcgta cgaaatcggc ctgcaacgcc tgcaaacgcg
    57181 cttcattgtg accaaaagtg catccccaca gaagcagact catcggccag cgtgccgcac
    57241 gaccttttat caataacgca atggctatga tcggcaacaa cggcagaaca agtaacgcat
    57301 tcagcggttg gcgacgcagt aaataacgca aaaaactgcc gaacatatcc tgctgatgca
    57361 atgttccatc taagtcaaaa aacaccacac gacgctcgtg agttgccaaa ccatactcct
    57421 ctggactgaa acatcctgat taattttcgc tactacatag cctaacagat agatcatcac
    57481 ttttccggca acaatcgacc gcatcatggc taactggaat ttttaattca tggcaattag
    57541 cggcaatgga atataaaatt cactcgcgtg tgtctcatat tgcctgttgt cgccattatt
    57601 tcatcgcgag gaaagatatg aacggcttac ttcgtatccg tcagcgttac caggggcttg
    57661 cccagagtga taaaaaactg gcggattatc tgctgctaca acctgatacg gcgcgccatt
    57721 taagctctca gcaactggcc aacgaagccg gagtcagtca gtccagcgtc gtgaagttcg
    57781 cgcaaaaact cggctataaa ggttttccgg cactaaagtt ggcgttgagt gaagcgctgg
    57841 caagccagcc ggaatctccc tccgtgccca ttcataacca aatccgcggt gatgatccgt
    57901 tacggctggt cggcgaaaaa ctgattaaag aaaatactgc cgctatgtac gcaacgttaa
    57961 acgttaatag tgaagagaaa ctgcatgaat gcgtggcaat gttgcgctct gcgcggcgga
    58021 taattctgac cggtattggc gcttcgggtc tggtggcgca aaactttgcc tggaagctga
    58081 tgaagattgg cttcaatgct gccgcagtgc gcgatatgca tgcgctactc gctacagtac
    58141 aggcgtcgtc ccctgacgat ctgttattag ccatttccta caccggtgta cgacgcgagt
    58201 taaacctggc ggcagatgag atgctgcgag tgggcggaaa agtgctggct attaccggct
    58261 ttactcccaa tgccctgcaa cagcgtgctt ctcattgcct gtataccatt gccgaagaac
    58321 aagcgacaaa cagtgcttca atctctgctt gtcacgcgca gggaatgtta acggatttgc
    58381 tgttcattgc gctgattcag caggatctgg aactggcacc agaacgtatt cgtcatagcg
    58441 aagcgctggt gaaaaaactg gtctgagtaa agaatgcgcg tataatgccc gcccggtttg
    58501 tgttgttttg agagtttcct tatggcgctg ttaatcacta aaaaatgcat caattgtgat
    58561 atgtgtgaac ccgaatgccc gaatgaggcg atttcgatgg gtgaacatat ctacgagatt
    58621 aacagcgata agtgtaccga atgcgtaggg cactacgaga caccaacctg ccagaaggtg
    58681 tgtccgatcc ccaatactat tgtgaaagat ccgcaacatg tcgagacgga agaacagttg
    58741 tgggataaat ttgtgctgat gcaccacgcg gataaaattt aatattctgc tctggaagta
    58801 gagtgttaat tattttatag aaaaatctta acgctattat atttatt
//
LOCUS       DADSYM010000006        54693 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2415
            SAMN08039579-rid11723173.denovo.006, whole genome shotgun sequence.
ACCESSION   DADSYM010000006 DADSYM010000000
VERSION     DADSYM010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039579
            Sequence Read Archive: SRR6362533
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 54693)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 54693)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980162.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 03:39:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,484
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 4,347
            CDSs (with protein)               :: 4,347
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 43 of 85
            Pseudo Genes (incomplete)         :: 37 of 85
            Pseudo Genes (internal stop)      :: 19 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..54693
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039579-rid11723173.denovo.006"
                     /strain="LGE2415"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8302 N 95.9628 W"
                     /collection_date="2015-08-24"
     gene            37..588
                     /locus_tag="J1Y83_000293"
     CDS             37..588
                     /locus_tag="J1Y83_000293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001683231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HBA7628482.1"
                     /translation="MQNIAVVYYSGYGHTKLVAEKVAQGAHADLISIDNEGNITEQEW
                     EKLDAADAIIFGAPTYMGGAPWQFKKFADASSKAWFTRKWQDKVFGGFTNSASLNGDK
                     QVTLIYLQTLASQHGGLWVSLGQAPANVLASTREDVNNLGGSVGLLVQSPADAGADQI
                     PTGDLDTAVKYGERVATITVRLK"
     gene            complement(766..1758)
                     /gene="rpoS"
                     /locus_tag="J1Y83_000294"
     CDS             complement(766..1758)
                     /gene="rpoS"
                     /locus_tag="J1Y83_000294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoS"
                     /protein_id="HBA7628483.1"
                     /translation="MSQNTLKVHDLNEDAEFDENGVEVFDEKALVEEEPSDNDLAEEE
                     LLSQGATQRVLDATQLYLGEIGYSPLLTAEEEVYFARRALRGDVASRRRMIESNLRLV
                     VKIARRYGNRGLALLDLIEEGNLGLIRAVEKFDPERGFRFSTYATWWIRQTIERAIMN
                     QTRTIRLPIHIVKELNVYLRTARELSHKLDHEPSAEEIAEQLDKPVDDVSRMLRLNER
                     ITSVDTPLGGDSEKALLDILADEKENGPEDTTQDDDMKQSIVKWLFELNAKQREVLAR
                     RFGLLGYEAATLEDVGREIGLTRERVRQIQVEGLRRLREILQTQGLNIEALFRE"
     gene            complement(1821..2960)
                     /gene="nlpD"
                     /locus_tag="J1Y83_000295"
     CDS             complement(1821..2960)
                     /gene="nlpD"
                     /locus_tag="J1Y83_000295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein hydrolase activator NlpD"
                     /protein_id="HBA7628484.1"
                     /translation="MSAGSPKFTVRRIAALSLVSLWLAGCSDTSNPPAPVSSVNGNAP
                     ANTNSGMLITPPPKMGTTSTAQQPQIQPVQQPQIQATQQPQIQPVQPVAQQPVQMENG
                     RIVYNRQYGNIPKGSYSGSTYTVKKGDTLFYIAWITGNDFRDLAQRNNIQAPYALNVG
                     QTLQVGNASGTPITGGNAITQADAAEQGVVIKPAQNSTVAVASQPTITYSESSGEQSA
                     NKMLPNNKPTATTVTAPVTVPTASTTEPTVSSTSTSTPISTWRWPTEGKVIETFGASE
                     GGNKGIDIAGSKGQAIIATADGRVVYAGNALRGYGNLIIIKHNDDYLSAYAHNDTMLV
                     REQQEVKAGQKIATMGSTGTSSTRLHFEIRYKGKSVNPLRYLPQR"
     gene            complement(3100..3726)
                     /gene="pcm"
                     /locus_tag="J1Y83_000296"
     CDS             complement(3100..3726)
                     /gene="pcm"
                     /locus_tag="J1Y83_000296"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate O-methyltransferase"
                     /protein_id="HBA7628485.1"
                     /translation="MVSRRVQALLDQLRAQGIQDELVLNALAAVPREKFVDEAFEQKA
                     WDNIALPIGQGQTISQPYMVARMTELLELTPQSRVLEIGTGSGYQTAILAHLVQHVCS
                     VERIKGLQWQARRRLKNLDLHNVSTRHGDGWQGWQARAPFDAIIVTAAPPEIPTALMT
                     QLDEGGILVLPVGEEHQYLKRVRRRGGEFIIDTVEAVRFVPLVKGELA"
     gene            complement(3720..4481)
                     /gene="surE"
                     /locus_tag="J1Y83_000297"
     CDS             complement(3720..4481)
                     /gene="surE"
                     /locus_tag="J1Y83_000297"
                     /EC_number="3.1.3.5"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HBA7628486.1"
                     /translation="MRILLSNDDGVHAPGIQTLAKALREFADVQVVAPDRNRSGASNS
                     LTLESSLRTFTFENGDIAVQMGTPTDCVYLGVNALMRPRPDIVVSGINAGPNLGDDVI
                     YSGTVAAAMEGRHLGFPALAVSLDGHKHYDTAAAVTCSILRALCKEPLRTGRILNINV
                     PDLPLDQIKGIRVTRCGTRHPADQVIPQQDPRGNTLYWIGPPGGKCDAGPGTDFAAVD
                     EGYVSITPLHVDLTAHSAQDVVSDWLNSVGVGTQW"
     gene            complement(4462..5511)
                     /gene="truD"
                     /locus_tag="J1Y83_000298"
     CDS             complement(4462..5511)
                     /gene="truD"
                     /locus_tag="J1Y83_000298"
                     /EC_number="5.4.99.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(13) synthase TruD"
                     /protein_id="HBA7628487.1"
                     /translation="MIEFDNLTYLHGKPQGTGLLKANPEDFVVVEDLGFEPDGEGEHI
                     LVRILKNGCNTRFVADALAKFLKIHAREVSFAGQKDKHAVTEQWLCARVPGKEMPDLS
                     AFQLEGCQVLEYARHKRKLRLGALKGNAFTLVLREVSNRDDVEQRLIDICVKGVPNYF
                     GAQRFGIGGSNLQGALRWAQTNTPVRDRNKRSFWLSAARSALFNQIVAERLKKADVNQ
                     VVDGDALQLAGRGSWFVATTEELAELQRRVNDKELMITAALPGSGEWGTQREALAFEQ
                     AAVAEETELQTLLVREKVEAARRAMLLYPQQLSWNWWDDVTVEIHFWLPAGSFATSVV
                     RELINTTGDYAHIAE"
     gene            complement(5508..5987)
                     /gene="ispF"
                     /locus_tag="J1Y83_000299"
     CDS             complement(5508..5987)
                     /gene="ispF"
                     /locus_tag="J1Y83_000299"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HBA7628488.1"
                     /translation="MRIGHGFDVHAFGGEGPIIIGGVRIPYEKGLLAHSDGDVALHAL
                     TDALLGAAALGDIGKLFPDTDPAFKGADSRELLREAWRRIQAKGYALGNVDVTIIAQA
                     PRMLPHIPQMRVFIAEDLGCHMDDVNVKATTTEKLGFTGRGEGIACEAVALLIKATK"
     gene            complement(5987..6697)
                     /gene="ispD"
                     /locus_tag="J1Y83_000300"
     CDS             complement(5987..6697)
                     /gene="ispD"
                     /locus_tag="J1Y83_000300"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HBA7628489.1"
                     /translation="MATTHLDVCAVVPAAGFGRRMQTECPKQYLSIGNQTILEHSVHA
                     LLAHPRVKRVVIAISPGDSRFAQLPLANHPRITVVDGGEERADSVLAGLKAAGDAQWV
                     LVHDAARPCLHQDDLARLLALSETSRTGGILAAPVRDTMKRAEPGKNAIAHTVDRNGL
                     WHALTPQFFPRELLHDCLTRALNEGATITDEASALEYCGFHPQLVEGRADNIKVTRPE
                     DLALAEFYLTRTIHQENT"
     gene            complement(6716..7027)
                     /gene="ftsB"
                     /locus_tag="J1Y83_000301"
     CDS             complement(6716..7027)
                     /gene="ftsB"
                     /locus_tag="J1Y83_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsB"
                     /protein_id="HBA7628490.1"
                     /translation="MGKLTLLLLAILVWLQYSLWFGKNGIHDYTRVNNDVAAQQATNA
                     KLKARNDQLFAEIDDLNGGQEALEERARNELSMTRPGETFYRLVPDASKRAQSAGQNN
                     R"
     gene            complement(7220..7543)
                     /locus_tag="J1Y83_000302"
     CDS             complement(7220..7543)
                     /locus_tag="J1Y83_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3561 family protein"
                     /protein_id="HBA7628491.1"
                     /translation="MRNSHNITLTNNDGLTEVEDTTWSLPGAVVGFLSWLCALAIPML
                     IYGSNTLLFFIYTWPFFLALMPVAVVVGIALHSLMDGKLRYSIVFTLATVGIMFGALF
                     MWLLG"
     gene            complement(7593..8198)
                     /gene="cysC"
                     /locus_tag="J1Y83_000303"
     CDS             complement(7593..8198)
                     /gene="cysC"
                     /locus_tag="J1Y83_000303"
                     /EC_number="2.7.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyl-sulfate kinase"
                     /protein_id="HBA7628492.1"
                     /translation="MALHDENVVWHSHPVTPQQREQHHGHRGVVLWFTGLSGSGKSTV
                     AGALEEALHKLGVSTYLLDGDNVRHGLCSDLGFSDADRKENIRRVGEVANLMVEAGLV
                     VLTAFISPHRAERQMVRERVGEGRFIEVFVDTPLAICEARDPKGLYKKARAGELRNFT
                     GIDSVYEAPESAEIHLNGEQLVTNLVQQLLDLLRQNDIIRS"
     gene            complement(8198..9625)
                     /gene="cysN"
                     /locus_tag="J1Y83_000304"
     CDS             complement(8198..9625)
                     /gene="cysN"
                     /locus_tag="J1Y83_000304"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysN"
                     /protein_id="HBA7628493.1"
                     /translation="MNTALAQQIANEGGVEAWMIAQQHKSLLRFLTCGSVDDGKSTLI
                     GRLLHDTRQIYEDQLSSLHNDSKRHGTQGEKLDLALLVDGLQAEREQGITIDVAYRYF
                     STEKRKFIIADTPGHEQYTRNMATGASTCELAILLIDARKGVLDQTRRHSFISTLLGI
                     KHLVVAINKMDLVDYSEETFTRIREDYLTFAGQLPGNLDIRFVPLSALEGDNVASQSE
                     SMPWYSGPTLLEVLETVEIQRVVDAQPMRFPVQYVNRPNLDFRGYAGTLASGRVEVGQ
                     RVKVLPSGVESNVARIVTFDGDREEAFAGEAITLVLTDEIDISRGDLLLAADEALPAV
                     QSASLDVVWMAEQPLSPGQSYDIKIAGKKTRARVDGIRYQVDINNLTQREVENLPLNG
                     IGLVDLTFDEPLVLDRYQQNPVTGGLIFIDRLSNVTVGAGMVHEPVSQATAAPSEFSA
                     FELELNALVRRHFPHWGARDLLGDK"
     gene            complement(9627..10535)
                     /gene="cysD"
                     /locus_tag="J1Y83_000305"
     CDS             complement(9627..10535)
                     /gene="cysD"
                     /locus_tag="J1Y83_000305"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysD"
                     /protein_id="HBA7628494.1"
                     /translation="MDQKRLTHLRQLEAESIHIIREVAAEFSNPVMLYSIGKDSSVML
                     HLARKAFYPGTLPFPLLHVDTGWKFREMYEFRDRTAKAYGCELLVHKNPEGVAMGINP
                     FVHGSAKHTDIMKTEGLKQALNKYGFDAAFGGARRDEEKSRAKERIYSFRDRFHRWDP
                     KNQRPELWHNYNGQINKGESIRVFPLSNWTEQDIWQYIWLENIDIVPLYLAAERPVLE
                     RDGMLMMIDDNRIDLQPGEVIKKRMVRFRTLGCWPLTGAVESNAQTLPEIIEEMLVST
                     TSERQGRVIDRDQAGSMELKKRQGYF"
     gene            10787..11824
                     /gene="iap"
                     /locus_tag="J1Y83_000306"
     CDS             10787..11824
                     /gene="iap"
                     /locus_tag="J1Y83_000306"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase isozyme conversion
                     aminopeptidase"
                     /protein_id="HBA7628495.1"
                     /translation="MFSALRHRTAALALGVCFILPVHASSPKPGDFANTQARHIATFF
                     PGRMTGTPAEMLSADYIRQQFQQMGYRSDIRTFNSRYIYTARDNRKSWHNVTGSTVIA
                     AHEGKAPQQIIIMAHLDTYAPLSDADADANLGGLTLQGMDDNAAGLGVMLELAERLKN
                     TPTEYGIRFVATSGEEEGKLGAENLLKRMSDTEKKNTLLVINLDNLIVGDKLYFNSGV
                     KTPEAVRKLTRDRALAIARSHGIAATTNPGLNKNYPKGTGCCNDAEIFDKAGITVLSV
                     EATNWNLGNKDGYQQRAKTAAFPAGNSWHDVRLDNQQHIDKALPGRIERRCRDVMRIM
                     LPLVKELAKAS"
     gene            complement(11949..12101)
                     /locus_tag="J1Y83_000307"
     CDS             complement(11949..12101)
                     /locus_tag="J1Y83_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Hok family toxin"
                     /protein_id="HBA7628496.1"
                     /translation="MLTKYALVAIIVLCCTVLGFTLMVGDSLCELSIRERGMEFKAVL
                     AYESKK"
     gene            complement(12366..13100)
                     /gene="cysH"
                     /locus_tag="J1Y83_000308"
     CDS             complement(12366..13100)
                     /gene="cysH"
                     /locus_tag="J1Y83_000308"
                     /EC_number="1.8.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase"
                     /protein_id="HBA7628497.1"
                     /translation="MSKLDLNALNELPKVDRILALAETNAQLEKLDAEGRVAWALDNL
                     PGEYVLSSSFGIQAAVSLHLVNQIRPDIPVILTDTGYLFPETYRFIDELTDKLKLNLK
                     VYRATESAAWQEARYGKLWEQGVEGIEKYNDINKVEPMNRALKELNVQTWFAGLRREQ
                     SGSRANLPVLAIQRGVFKVLPIIDWDNRTIYQYLQKHGLKYHPLWDEGYLSVGDTHTT
                     RKWEPGMAEEETRFFGLKRECGLHEG"
     gene            complement(13174..14886)
                     /gene="cysI"
                     /locus_tag="J1Y83_000309"
     CDS             complement(13174..14886)
                     /gene="cysI"
                     /locus_tag="J1Y83_000309"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001290736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="assimilatory sulfite reductase (NADPH)
                     hemoprotein subunit"
                     /protein_id="HBA7628498.1"
                     /translation="MSEKHPGPLVVEGKLTDAERMKLESNYLRGTIAEDLNDGLTGGF
                     KGDNFLLIRFHGMYQQDDRDIRAERAEQKLEPRHAMLLRCRLPGGVITTKQWQAIDKF
                     AGENTIYGSIRLTNRQTFQFHGILKKNVKPVHQMLHSVGLDALATANDMNRNVLCTSN
                     PYESQLHAEAYEWAKKISEHLLPRTRAYAEIWLDQEKVATTDEEPILGQAYLPRKFKT
                     TVVIPPQNDIDLHANDMNFVAIAENGKLVGFNLLVGGGLSIEHGNKKTYARTASEFGY
                     LPLEHTLAVAEAVVTTQRDWGNRTDRKNAKTKYTLERVGVETFKAEVERRAGIKFEPI
                     RPYEFTGRGDRIGWVKGIDDNWHLTLFIENGRILDYPGRPLKTGLLEIAKIHKGDFRI
                     TANQNLIIAGVPESEKAKIEKIAKESGLMNAVTPQRENSMACVSFPTCPLAMAEAERF
                     LPSFIDNIDNLMAKHGVSDEHIVMRVTGCPNGCGRAMLAEVGLVGKAPGRYNLHLGGN
                     RIGTRIPRMYKENITEPEILASLDELIGRWAKEREVGEGFGDFTVRAGIIRPVLDPAR
                     DLWD"
     gene            complement(14886..16685)
                     /gene="cysJ"
                     /locus_tag="J1Y83_000310"
     CDS             complement(14886..16685)
                     /gene="cysJ"
                     /locus_tag="J1Y83_000310"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent assimilatory sulfite reductase
                     flavoprotein subunit"
                     /protein_id="HBA7628499.1"
                     /translation="MTTQVPPSALLPLNPEQLARLQAATTDLTPTQLAWVSGYFWGVL
                     NQQPAALAATPAPAAEMPGITIISASQTGNARRVAEALRDDLLAAKLNVKLVNAGDYK
                     FKQIASEKLLIVVTSTQGEGEPPEEAVALHKFLFSKKAPKLENTAFAVFSLGDSSYEF
                     FCQSGKDFDSKLAELGGERLLDRVDADVEYQAAASEWRARVVDALKSRAPVAAPSQSV
                     ATGAVNEIHTSPYSKDAPLAASLSVNQKITGRNSEKDVRHIEIDLGDSGLRYQPGDAL
                     GVWYQNDPALVKELVELLWLKGDEPVTVEGKTLPLNEALQWHFELTVNTANIVENYAT
                     LTRSETLLPLVGDKAKLQHYAATTPIVDMVRFSPAQLDAEALINLLRPLTPRLYSIAS
                     SQAEVENEVHVTVGVVRYDVEGRARAGGASSFLADRVEEEGEVRVFIEHNDNFRLPTN
                     PETPVIMIGPGTGIAPFRAFMQQRAADEAPGKNWLFFGNPHFTEDFLYQVEWQRYVKE
                     GVLTRIDLAWSRDQKEKIYVQDKLREQGAELWRWINDGAHIYVCGDANRMAKDVEQAL
                     LEVIAEFGGMDTEAADEFLSELRVERRYQRDVY"
     gene            17001..17366
                     /gene="queD"
                     /locus_tag="J1Y83_000311"
     CDS             17001..17366
                     /gene="queD"
                     /locus_tag="J1Y83_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase QueD"
                     /protein_id="HBA7628500.1"
                     /translation="MMSTTLFKDFTFEAAHRLPHVPQGHKCGRLHGHSFMVRLEITGE
                     VDPHTGWIIDFAELKAAFKPTYERLDHHYLNDIPGLENPTSEVLAKWIWDQVKPVVPL
                     LSAVMVKETCTAGCIYRGE"
     gene            17444..18715
                     /locus_tag="J1Y83_000312"
     CDS             17444..18715
                     /locus_tag="J1Y83_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HBA7628501.1"
                     /translation="MEDDCDIIIIGAGIAGTACALRCARAGLSVLLLERAEIPGSKNL
                     SGGRLYTHALAELLPQFHLTAPLERRITHESLSLLTPDGATTFSSLLPGGESWSVLRA
                     RFDPWLVAEAEKEGVECIPGATVDALYEENGRVCGVICGDDILRARYVVLAEGANSVL
                     AERHGLVTRPAGEAMALGIKEALALEPSAIEERFHLENNEGAALLFSGGICDDLPGGA
                     FLYTNQQTLSLGIVCPLSSLTQSRVPASELLARFKTHPAVRPLIKNTESLEYGAHLVP
                     EGGLRSMPVQYAGNGWLLVGDALRSCVNTGISVRGMDMALTGAQAAAQTLISACQHRE
                     PQNLFPLYHHNVERSLLWDVLQRYQHVPALLQRPGWYRVWPGLMQDISRDLWDQGNTP
                     VPPLRQLLWRHLRRHGLWHLTGDVIRSLRCL"
     gene            18706..18966
                     /locus_tag="J1Y83_000313"
     CDS             18706..18966
                     /locus_tag="J1Y83_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HBA7628502.1"
                     /translation="MSVARNLWRAADAPHIVPADSVERQTAQRLINACPAGLFSLTPE
                     GDLRVDYHGCLECGTCRLLCDESTLQQWRYPPSGFGITYRFG"
     gene            18983..19558
                     /locus_tag="J1Y83_000314"
     CDS             18983..19558
                     /locus_tag="J1Y83_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate responsive antiterminator"
                     /protein_id="HBA7628503.1"
                     /translation="MPLLHLLRQNPVIAAVKDNASLQLAIDSECQFISVLYGNICTIS
                     NIVKKIKNAGKYAFIHVDLLEGASNKEVVIQFLKLVTEADGIISTKASMLKAARAEGF
                     FCIHRLFIVDSISFHNIDKQVAQSNPDCIEILPGCMPKVLGWVTEKIRQPLIAGGLVC
                     DEEDARNAINAGVVALSTTNTGVWTLAKKLL"
     gene            complement(19706..20566)
                     /locus_tag="J1Y83_000315"
     CDS             complement(19706..20566)
                     /locus_tag="J1Y83_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417249.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HBA7628504.1"
                     /translation="MNIVLVTINQDHAAIASWLAAQDFSGCTLAHWQIEPQPMIAEQV
                     LDALVEQWQRTPADVVLFPPGTFGDELSTRLAWRLHGASICQVTSLDIPTVSVRKSHW
                     GNALTATLQTEKRPLCLSLARQAGADKNATLPSGMQQLNIVPGALPDWLISVENLKNV
                     TRDPLAEARRVLVVGQGGEADNQEIAMLAEKLGAEVGYSRARVMNGGVDAEKVIGISG
                     HLLAPEVCIVVGASGAAALMAGVRNSKFVVAINHDASAAVFSQADVGVVDDWKVVLEA
                     LVTTIHADCQ"
     gene            complement(20563..21342)
                     /locus_tag="J1Y83_000316"
     CDS             complement(20563..21342)
                     /locus_tag="J1Y83_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HBA7628505.1"
                     /translation="MNILLAFKAEPDAGMLAEKEWLAAAQGNSGPDVSLLRSLLGADE
                     QAAAALLLAQRKNGTPMSLTALSMGDERALHWLRYLMALGFEDAVLLETAADLRFAPE
                     FVARHIAEWQYQNPLDLIITGCQSSEGQNGQTPFLLAEMLVWPCFTQVERFTLDAPFI
                     TLEQRTEHGVRCCRVRLPAVIAVRQCGEVALPVPGMRQRMAAGKAEITRETVAAEAPA
                     IQCLQLARPEQRRGATLIDGQTVAEKAQKLWQDYLCQRMQP"
     gene            complement(21320..22729)
                     /locus_tag="J1Y83_000317"
     CDS             complement(21320..22729)
                     /locus_tag="J1Y83_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBA7628506.1"
                     /translation="MTGRCLFGFSGEKPFLLPDNEGVKMNTSPVRMDDLPLNRFHCRI
                     AALTFGAHLTDGYVLGVIGYAIIQLTPAMQLTPFMAGMIGGSALLGLFLGSLVLGWIS
                     DHIGRQKIFTFSFMLITLASFLQFFATTPEHLIGLRILIGIGLGGDYSVGHTLLAEFS
                     PRRHRGVLLGAFSVVWTVGYVLASIAGHHFISESPEAWRWLLASAALPALLITLLRWG
                     TPESPRWLLRQGRFAEAHAIVHRYFGSHVLLGDEVATATHKHIKTLFSSRYWRRTAFN
                     SVFFVCLVIPWFVIYTWLPTIAQTIGLEDALTASLMLNALLIVGALLGLVLTHLLAHR
                     KFLLGSFLLLAATLVVMACLPSGSSLTLLLFVLFSTTISAVSNLVGILPAESFPTDIR
                     SLGVGFATAMSRLGAAVSTGLLPWVLAQWGMQATLLLLAAVLLVGFVVTWLWAPETKA
                     LPLVAAGNVGGANEHSVSV"
     gene            complement(22751..24205)
                     /locus_tag="J1Y83_000318"
     CDS             complement(22751..24205)
                     /locus_tag="J1Y83_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HBA7628507.1"
                     /translation="MSLSRVAIVDQLKEIVGADRVITDETVLKKNSIDRFRKFPDIHG
                     IYTLPIPAAVVKLGSTEQVSRVLNFMNAHKINGVPRTGASATEGGLETVVENSVVLDG
                     SAMNQIINIDIENMQATAQCGVPLEVLENALREKGYTTGHSPQSKPLAQMGGLVATRS
                     IGQFSTLYGAIEDMVVGLEAVLADGTVTRIKNVPRRAAGPDIRHIIIGNEGALCYITE
                     VTVKIFKFTPENNLFYGYVLEDMKTGFNILREIMVEGYRPSIARLYDAEDGTQHFTHF
                     ADGKCVLIFMAEGNPRIAKATGEGIAEIVARYPQCQRVDSKLIETWFNNLNWGPDKVA
                     AERVQILKTGNMGFTTEVSGCWSCIHEIYESVINRIRTEFPYADDITMLGGHSSHSYQ
                     NGTNMYFVYDYNVVDCKPEEEIDKYHNPLNKIICEETIRLGGSMVHHHGIGKHRVHWS
                     KLEHGSAWALLEGLKKQFDPNGIMNTGTIYPIEK"
     gene            complement(24275..25060)
                     /locus_tag="J1Y83_000319"
     CDS             complement(24275..25060)
                     /locus_tag="J1Y83_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311657.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HBA7628508.1"
                     /translation="MSIESLNAFSMDFFSLKGKTAIVTGGNSGLGQAFAMALAKAGAN
                     VFIPSFVKDNGETKEMIEKQGVEVDFMQVDITAEGAPQKIIAACCERFGTVDILVNNA
                     GICKLNKVLDFGRTDWDPMIDVNLTAAFELSYEAAKIMIPQKSGKIINICSLFSYLGG
                     QWSPAYSATKHALAGFTKAYCDELGQYNIQVNGIAPGYYATDITLATRSNPETNQRVL
                     DHIPANRWGDTQDLMGAAVFLASPASNYVNGHLLVVDGGYLVR"
     gene            25379..26656
                     /locus_tag="J1Y83_000320"
                     /pseudo
     CDS             25379..26656
                     /locus_tag="J1Y83_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311658.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            26683..28161
                     /locus_tag="J1Y83_000321"
     CDS             26683..28161
                     /locus_tag="J1Y83_000321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HBA7628509.1"
                     /translation="MSKKYIIGIDGGSQSTKVVMYDLEGNVVCEGKGLLQPMHTPDAD
                     TAEHPDDDLWASLCFASHDLMSQFAGNKEDIVGIGLGSIRCCRALLKADGTPAAPLIS
                     WQDARVTRPYEHTNPDVAYVTSFSGYLTHRLTGEFKDNIANYFGQWPVDYKSWAWSEN
                     AAVMEKFNIPRQMLFDVQMPGTVLGHITPQAALATHFPAGLPVVCTTSDKPVEALGAG
                     LLDDETAVISLGTYIALMMNGKALPKDPVAYWPIMSSIPQTLLYEGYGIRKGMWTVSW
                     LRDMLGESLIQDAKAQDLSPEDLLNKKASCVPPGCNGLMTVLDWLTNPWEPYKRGIMI
                     GFDSSMDYAWIYRSILESVALTLKNNYDNMCHEMNHFAKHVIITGGGSNSDLFMQIFA
                     DVFNLPTRRNAINGCASLGAAINTAVGLGLYPDYTKAVDKMVRVKDIFIPIESNAKRY
                     DAMNKGIFKDLTKHTDVILKKSYEVMHGELGNADSIQSWSNA"
     gene            28326..29246
                     /locus_tag="J1Y83_000322"
     CDS             28326..29246
                     /locus_tag="J1Y83_000322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001461526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoimidazole riboside kinase"
                     /protein_id="HBA7628510.1"
                     /translation="MSTRVWVLGDAVVDLLPESQGRLLQCPGGAPANVAVGIARLGGE
                     SAFIGKVGDDPFGRFMYQTLSKENVDTRYMSLDPQHRTSIVAVGLDEQGERNFTFMVR
                     PSADLFLQPDDLPTFGPGEWLHLCSIALSAEPSRSTAFLAMEKIRQAGGNISFDPNIR
                     SDLWQSEALLRKYLDRALSLANIAKLSEEELLFISGESQVQQGAYSLVQRYSLTLLLI
                     TQGKNGVLVYFQGQFIHYPAKPVSVVDTTGAGDAFVAGLLAGLADSGIPTNTRQLERI
                     IAQAQICGALATTAKGAMTALPRQHDLPSQ"
     gene            29414..30931
                     /locus_tag="J1Y83_000323"
     CDS             29414..30931
                     /locus_tag="J1Y83_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003857084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate porin"
                     /protein_id="HBA7628511.1"
                     /translation="MYKKTTLAVLIALLTGATTVHAQTDISSIESRLAALEQRLKNAE
                     SRAQAAEARAKTAELQVQKLAETQQQNQLTTQEVAQRTVQLEQKSAENSGFEFHGYAR
                     SGLLMNDAASSSKSGPYLTPAGETGGAVGRLGNEADTYVELNVEHKQTLDNGATTRFK
                     AMLADGQRDYNDWTGGSSNLNIRQAFAELGALPSFTGAFKDSTVWAGKRFDRDNFDIH
                     WLDSDVVFLAGTGGGIYDVKWNDTFRSNFSLYGRNFGDLDDIDNNVQNYILTMNHYAG
                     PFQLMVSGLRAKDNDDRKDANGDLIQTDAANTGVHALVGLHNDTFYGLREGTAKTALL
                     YGHGLGAEVKGIGSDGALLSEANTWRFASYGTTPLGSGWYVAPAILAQSSKDRYVKGD
                     SYEWVTFNTRLIKEVTQNFALAFEGSYQYMDLKPKGYQNHNAVNGSFYKLTFAPTLKA
                     NDINNFFSRPELRLFATWMDWSSKLDDFASNDAFGSSGFNTGGEWNFGVQMETWF"
     gene            30992..32362
                     /locus_tag="J1Y83_000324"
     CDS             30992..32362
                     /locus_tag="J1Y83_000324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005225625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sucrose transporter subunit IIBC"
                     /protein_id="HBA7628512.1"
                     /translation="MDFDKIAQSLLPLLGGKENIASAAHCATRLRLVLVDDTLADQHA
                     IGQIDGVKGCFRNSGQMQIIFGTGVVNKVYAAFIQVAGISESSKADTARLAAQKLNPF
                     QRIARLLSNIFVPIIPAIVASGLLMGLLGMVKTYGWVNADNAIYILLDMCSSAAFIIL
                     PILIGFTAAREFGGNPYLGATLGGILTHPALTNAWGVAAGFQTMNFFGFEIAMIGYQG
                     TVFPVLLAVWFMSIVEKQLRRFIPDALDLILTPFLTVVISGFIALLIIGPAGRALGDG
                     ISFVLSTLIAHAGWLAGLLFGGLYSAIVITGIHHSFHAIEAGLLGNPAIGVNFLLPIW
                     AMANVAQGGACLAVWFKTKDTKIKAITLPSAFSAMLGITEAAIFGINLRFVKPFIAAL
                     IGGAAGGAWVVSVHVYMTAVGLTAIPGMAIVQPTSLVNYIIGMVIAFAVAFSLSLLLK
                     YKTDEE"
     gene            32362..33771
                     /locus_tag="J1Y83_000325"
     CDS             32362..33771
                     /locus_tag="J1Y83_000325"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004111110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HBA7628513.1"
                     /translation="MSMPSQMPAILQAVMKGQSKALADAHYPCWHLAPVTGLMNDPNG
                     FCWSGGRYHLFYQWNPLACDHKYKCWGHWSSTDLLHWQHEPLALMPDKEYDRNGCYSG
                     SAVNNQGVLTLCYTGNVKFDDGSRTAWQCLATENNQGGFDKLGPVIPLPDGYTGHVRD
                     PKVWKHNSQWYMVLGAQDKEKRGKVLLYSSVDLYTWSFHGEIAGNGLNEIDNAGYMWE
                     CPDFFALDGEYILLCCPQGMVREHERYLNTYPCAWLHGQFDYETGKFTHGAFSELDAG
                     FEFYAPQTMEAPDGRRLLVGWMGVPDGEEMLQPTRKHHWQHQMTCFRELSFQQGKLFQ
                     MPIRELAQLREAEHFWQGKADHAPHVEIERLEMDIIPSGELYLNFGNALALHLNDDGI
                     QLQRRSLAGQEKLTRYWRGSVTSLKILCDSSSVEIFINNGEGVMSNRYFPHHPASLIL
                     QGESDVTLRYWSLRACMVE"
     gene            33797..34804
                     /locus_tag="J1Y83_000326"
     CDS             33797..34804
                     /locus_tag="J1Y83_000326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001559786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBA7628514.1"
                     /translation="MRKTKRVTIKDIAELAGVSKATASLVLNGRGKELRVAQETRERV
                     LSIAQQQHYQPSIHARSLRDNRSHTIGLVVPEITNYGFAVFSHELETLCREAGVQLLI
                     SCTDENPSQETMVVNNMIARQVDGLIVASSMLHDNDYQKLSEQLPVVLFDRHMNGSTL
                     PLVITDSVSPTAALVADIARSHPDEFYFLGGQPRLSPTRDRLEGFTQGLQQAGVTLQP
                     EWIIHGNYHPSSGYEMFAALCARLGRPPKALFTAACGLLEGVLRYMSQYNLLDSKIHL
                     ASFDDHYLYDSLSVRIDTIQQDNRQLAFHCFELISQLIEGETPSPLQRYLPASLQKRY
                     R"
     gene            complement(34889..35560)
                     /gene="queE"
                     /locus_tag="J1Y83_000327"
     CDS             complement(34889..35560)
                     /gene="queE"
                     /locus_tag="J1Y83_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-carboxy-7-deazaguanine synthase QueE"
                     /protein_id="HBA7628515.1"
                     /translation="MQYPINEMFQTLQGEGYFTGVPAIFIRLQGCPVGCAWCDTKHTW
                     EKLEDREVSLFSILAKTKESDKWGAASSEDLLAVIGRQGYTARHVVITGGEPCIHDLL
                     PLTDLLEKNGFSCQIETSGTHEVRCTPNTWVTVSPKLNMRGGYEVLSQALERANEIKH
                     PVGRVRDIEALDELLATLTDDKPRVIALQPISQKEDATRLCIDTCIARNWRLSMQTHK
                     YLNIA"
     gene            35699..35840
                     /locus_tag="J1Y83_000328"
                     /pseudo
     CDS             35699..35840
                     /locus_tag="J1Y83_000328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791252.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            35854..36726
                     /locus_tag="J1Y83_000329"
     CDS             35854..36726
                     /locus_tag="J1Y83_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417258.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgcG family protein"
                     /protein_id="HBA7628516.1"
                     /translation="MRYFILIFTLVCSFVAAQPTIVPQLQQQVTDLTSSLNSQEKKEL
                     TRKLESIFNNTQVQIAVLIIPTTKDETIEQYAARVFDNWRLGDAKRNDGILIIVAWSD
                     RTVRIQVGYGLEEKVTDALAGDIIRSNMIPAFKQQKLAKGLELAIIALNNRLTSQHQY
                     SVNPSESESASSSDHYYFAIFWVFAVMFFPFWFFHQDSNFCRVCKSSVCISAIYLLDL
                     FLFSDKTFSIAVFFFFFTFTTIMVFTCLCVLQKKASGRSYHSDNSGSGGGSSGGGFSG
                     GGGSSGGGGASGRW"
     gene            complement(36786..38084)
                     /gene="eno"
                     /locus_tag="J1Y83_000330"
     CDS             complement(36786..38084)
                     /gene="eno"
                     /locus_tag="J1Y83_000330"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HBA7628517.1"
                     /translation="MSKIVKIIGREIIDSRGNPTVEAEVHLEGGFVGMAAAPSGASTG
                     SREALELRDGDKSRFLGKGVTKAVAAVNGPIAQALIGKDAKDQAGIDKIMIDLDGTEN
                     KSKFGANAILAVSLANAKAAAAAKGMPLYEHIAELNGTPGKYSMPVPMMNIINGGEHA
                     DNNVDIQEFMIQPVGAKTVKEAIRMGSEVFHHLAKVLKAKGMNTAVGDEGGYAPNLGS
                     NAEALAVIAEAVKAAGYELGKDITLAMDCAASEFYKDGKYVLAGEGNKAFTSEEFTHF
                     LEELTKQYPIVSIEDGLDESDWDGFAYQTKVLGDKIQLVGDDLFVTNTKILKEGIEKG
                     IANSILIKFNQIGSLTETLAAIKMAKDAGYTAVISHRSGETEDATIADLAVGTAAGQI
                     KTGSMSRSDRVAKYNQLIRIEEALGEKAPYNGRKEIKGQA"
     gene            complement(38172..39809)
                     /gene="pyrG"
                     /locus_tag="J1Y83_000331"
     CDS             complement(38172..39809)
                     /gene="pyrG"
                     /locus_tag="J1Y83_000331"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase (glutamine hydrolyzing)"
                     /protein_id="HBA7628518.1"
                     /translation="MTTNYIFVTGGVVSSLGKGIAAASLAAILEARGLNVTIMKLDPY
                     INVDPGTMSPIQHGEVFVTEDGAETDLDLGHYERFIRTKMSRRNNFTTGRIYSDVLRK
                     ERRGDYLGATVQVIPHITNAIKERVLEGGEGHDVVLVEIGGTVGDIESLPFLEAIRQM
                     AVEIGREHTLFMHLTLVPYMAASGEVKTKPTQHSVKELLSIGIQPDILICRSDRAVPA
                     NERAKIALFCNVPEKAVISLKDVDSIYKIPGLLKSQGLDDYICKRFSLDCPEANLSEW
                     EQVIFEEANPVSEVTIGMVGKYIELPDAYKSVIEALKHGGLKNRVSVNIKLIDSQDVE
                     TRGVEILKGLDAILVPGGFGYRGVEGMITTARFARENNIPYLGICLGMQVALIDYARH
                     VANMENANSTEFVPDCKYPVVALITEWRDENGNVEVRSEKSDLGGTMRLGAQQCQLVD
                     DSLVRQLYNAPTIVERHRHRYEVNNMLLKQIEDAGLRVAGRSGDDQLVEIIEVPNHPW
                     FVACQFHPEFTSTPRDGHPLFAGFVKAASEFQKRQAK"
     gene            complement(40037..40828)
                     /gene="mazG"
                     /locus_tag="J1Y83_000332"
     CDS             complement(40037..40828)
                     /gene="mazG"
                     /locus_tag="J1Y83_000332"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HBA7628519.1"
                     /translation="MNQIDRLLTIMQRLRDPENGCPWDKEQTFATIAPYTLEETYEVL
                     DAIAREDFDDLRGELGDLLFQVVFYAQMAQEEGRFDFNDICAAISDKLERRHPHVFAD
                     SSAENSSEVLARWEQIKTEERAQKAQHSALDDIPRSLPALMRAQKIQKRCANVGFDWT
                     TLGPVVDKVYEEIDEVMYEARQAVVDQAKLEEEMGDLLFATVNLARHLGTKAEIALQK
                     ANEKFERRFREVERIVAARGLEMTGVDLETMEEVWQQVKRQEIDL"
     gene            complement(40900..41235)
                     /gene="mazF"
                     /locus_tag="J1Y83_000333"
     CDS             complement(40900..41235)
                     /gene="mazF"
                     /locus_tag="J1Y83_000333"
                     /EC_number="3.1.27.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endoribonuclease MazF"
                     /protein_id="HBA7628520.1"
                     /translation="MVSRYVPDTGDLIWVDFDPTKGSEQAGHRPAVVLSPFMYNNKTG
                     MCLCVPCTTQSKGYPFEVVLSGQERDGVALADQVKSIAWRARGATKKGTVAPEELQLI
                     KAKINVLIG"
     gene            complement(41235..41483)
                     /gene="mazE"
                     /locus_tag="J1Y83_000334"
     CDS             complement(41235..41483)
                     /gene="mazE"
                     /locus_tag="J1Y83_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin MazE"
                     /protein_id="HBA7628521.1"
                     /translation="MIHSSVKRWGNSPAVRIPATLMQALNLNIDDEVKIDLVDGKLII
                     EPVRKEPVFTLAELVNDITPENLHENIDWGEPKDKEVW"
     gene            complement(41561..43795)
                     /locus_tag="J1Y83_000335"
     CDS             complement(41561..43795)
                     /locus_tag="J1Y83_000335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase"
                     /protein_id="HBA7628522.1"
                     /translation="MVAVRSAHINKAGEFDPEKWIASLGITSQKSCECLAETWAYCLQ
                     QTQGHPDASLLLWRGVEMVEILSTLSMDIDTLRAALLFPLADANVVSEDVLRESVGKS
                     VVNLIHGVRDMAAIRQLKATHTDSVSSEQVDNVRRMLLAMVDDFRCVVIKLAERIAHL
                     REVKDAPEDERVLAAKECTNIYAPLANRLGIGQLKWELEDYCFRYLHPTEYKRIAKLL
                     HERRLDREHYIEEFVGHLRAEMKAEGVKAEVYGRPKHIYSIWRKMQKKNLAFDELFDV
                     RAVRIVAERLQDCYAALGIVHTHYRHLPDEFDDYVANPKPNGYQSIHTVVLGPGGKTV
                     EIQIRTKQMHEDAELGVAAHWKYKEGAAAGGARSGHEDRIAWLRKLIAWQEEMADSGE
                     MLDEVRSQVFDDRVYVFTPKGDVVDLPAGSTPLDFAYHIHSDVGHRCIGAKIGGRIVP
                     FTYQLQMGDQIEIITQKQPNPSRDWLNPNLGYVTTSRGRSKIHAWFRKQDRDKNILAG
                     RQILDDELEHLGISLKEAEKHLLPRYNFNDVDELLAAIGGGDIRLNQMVNFLQSQFNK
                     PSAEEQDAAALKQLQQKSYTPQNRSKDNGRVVVEGVGNLMHHIARCCQPIPGDEIVGF
                     ITQGRGISVHRADCEQLAELRSHAPERIVDAVWGESYSAGYSLVVRVVANDRSGLLRD
                     ITTILANEKVNVLGVASRSDTKQQLATIDMTIEIYNLQVLGRVLGKLNQVPDVIDARR
                     LHGS"
     gene            complement(43843..45144)
                     /gene="rlmD"
                     /locus_tag="J1Y83_000336"
     CDS             complement(43843..45144)
                     /gene="rlmD"
                     /locus_tag="J1Y83_000336"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HBA7628523.1"
                     /translation="MAQFYSAKRRTTTRQIITVSVNDLDSFGQGVARHNGKTLFIPGL
                     LPQENAEVTVTEDKKQYARAKVVRRLSDSPERETPRCPHFGVCGGCQQQHASVDLQQR
                     SKSAALARLMKHEVSEVIADVPWGYRRRARLSLNYLPKTQQLQMGFRKAGSSDIVDVK
                     QCPILVPQLEALLPKVRACLGSLQAMRHLGHVELVQATSGTLMILRHTAPLSSADREK
                     LECFSHSEGLDLYLAPDSEILETVSGEMPWYDSNGLRLTFSPRDFIQVNAGVNQKMVA
                     RALEWLDVEPEDRVLDLFCGMGNFTLPLATQAASVVGVEGVPALVEKGQQNARLNGLQ
                     NVTFYHENLEEDVTKQPWAKNGFDKVLLDPARAGAAGVMQQIIKLEPIRIVYVSCNPA
                     TLARDSEALLKAGYTIARLAMLDMFPHTGHLESMVLFSRVK"
     gene            45201..47957
                     /gene="barA"
                     /locus_tag="J1Y83_000337"
     CDS             45201..47957
                     /gene="barA"
                     /locus_tag="J1Y83_000337"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase BarA"
                     /protein_id="HBA7628524.1"
                     /translation="MTNYSLRARMMILILAPTVLIGLLLSIFFVVHRYNDLQRQLEDA
                     GASIIEPLAVSTEYGMSLQNRESIGQLISVLHRRHSDIVRAISVYDENNRLFVTSNFH
                     LDPSSMQLGSNVPFPRQLTVTRDGDIMILRTPIISESYSPDESPSSDAKNSQNMLGYI
                     ALELDLKSVRLQQYKEIFISSVMMLFCIGIALIFGWRLMRDVTGPIRNMVNTVDRIRR
                     GQLDSRVEGFMLGELDMLKNGINSMAMSLAAYHEEMQHNIDQATSDLRETLEQMEIQN
                     VELDLAKKRAQEAARIKSEFLANMSHELRTPLNGVIGFTRLTLKTELTPTQRDHLNTI
                     ERSANNLLAIINDVLDFSKLEAGKLILESIPFPLRSTLDEVVTLLAHSSHDKGLELTL
                     NIKSDVPDNVIGDPLRLQQIITNLVGNAIKFTENGNIDILVEKRALSNTKVQIEVQIR
                     DTGIGIPERDQSRLFQAFRQADASISRRHGGTGLGLVITQKLVNEMGGDISFHSQPNR
                     GSTFWFHINLDLNPNIIIEGPSTQCLAGKRLAYVEPNSAAAQCTLDILSETPLEVVYS
                     PTFSALPPAHYDMMLLGIAVTFREPLTMQHERLAKAVSMTDFLMLALPCHAQVNAEKL
                     KQDGIGACLLKPLTPTRLLPALTEFCHHKQNTLLPVTDESKLAMTVMAVDDNPANLKL
                     IGALLEDMVQHVELCDSGHQAVERAKQMPFDLILMDIQMPDMDGIRACELIHQLPHQQ
                     QTPVIAVTAHAMAGQKEKLLGAGMSDYLAKPIEEERLHNLLLRYKPGSGISSRVVTPE
                     VNEIVVNPNATLDWQLALRQAAGKTDLARDMLQMLLDFLPEVRNKVEEQLVGENPEGL
                     VDLIHKLHGSCGYSGVPRMKNLCQLIEQQLRSGTKEEDLEPELLELLDEMDNVAREAS
                     KILG"
     gene            complement(48188..49528)
                     /gene="gudD"
                     /locus_tag="J1Y83_000338"
     CDS             complement(48188..49528)
                     /gene="gudD"
                     /locus_tag="J1Y83_000338"
                     /EC_number="4.2.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucarate dehydratase"
                     /protein_id="HBA7628525.1"
                     /translation="MSSQFTTPVVTEMQVIPVAGHDSMLMNLSGAHAPFFTRNIVIIK
                     DNSGHTGVGEIPGGEKIRKTLEDAIPLVVGKTLGEYKNVLTLVRNTFADRDAGGRGLQ
                     TFDLRTTIHVVTGIEAAMLDLLGQHLGVNVASLLGDGQQRSEVEMLGYLFFVGNRKAT
                     PLPYQSQPDDSCDWYRLRHEEAMTPDAVVRLAEAAYEKYGFNDFKLKGGVLAGEEEAE
                     SIVALAQRFPQARITLDPNGAWSLKEAIKIGTYLKGSLAYAEDPCGAEQGFSGREVMA
                     EFRRATGLPTATNMIATDWRQMGHTLSLQSVDIPLADPHFWTMQGSVRVAQMCHEFGL
                     TWGSHSNNHFDISLAMFTHVAAAAPGKITAIDTHWIWQEGNQRLTKEPLEIKGGLVQV
                     PEKPGLGVEIDMDQVMKAHELYQKHGLGARDDAMGMQYLIPGWTFDNKRPCMVR"
     gene            complement(49549..50889)
                     /locus_tag="J1Y83_000339"
     CDS             complement(49549..50889)
                     /locus_tag="J1Y83_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucarate dehydratase-related protein"
                     /protein_id="HBA7628526.1"
                     /translation="MTTQSSPVITDMKVIPVAGHDSMLLNIGGAHNAYFTRNIVVLTD
                     NAGHTGIGEAPGGEVIYQTLVEAIPMVLGQEVARLNKVVQQVHKGNQAADFDTFGKGA
                     WTFELRVNAVAALEAALLDLLGQALNVPVCELLGPGKQRDAITVLGYLFYIGDRTKTD
                     LPYLENTPGNHEWYQLRHQKAMNSEAVVRLAEASQDRYGFKDFKLKGGVLPGEQEIDT
                     VRTLKKRFPDARITVDPNGAWLLDEAISLCKGLNDVLTYAEDPCGAEQGFSGREVMAE
                     FRRATGLPVATNMIATNWREMGHAVMLNAVDIPLADPHFWTLSGAVRVAQLCDDWGLT
                     WGCHSNNHFDISLAMFTHVGAAAPGNPTAIDTHWIWQEGDCRLTKNPLEIKNGKIAVP
                     DAPGLGVELDWEQVQKAHEAYKRLPGGARNDAGPMQYLIPGWTFDRKRPVFGRH"
     gene            complement(50891..52243)
                     /locus_tag="J1Y83_000340"
     CDS             complement(50891..52243)
                     /locus_tag="J1Y83_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBA7628527.1"
                     /translation="MSSLSQAASSVEKRTNARYWIVVMLFIVTSFNYGDRATLSIAGS
                     EMAKDIGLDPVGMGYVFSAFSWAYVIGQIPGGWLLDRFGSKRVYFWSIFIWSMFTLLQ
                     GFVDIFSGFGIIVALFTLRFLVGLAEAPSFPGNSRIVAAWFPAQERGTAVSIFNSAQY
                     FATVIFAPIMGWLTHEVGWSHVFFFMGGLGIVISFIWLKVIHEPNQHPGVNQKELEYI
                     AAGGALINMDQQNTKVKVPFSVKWGQIKQLLGSRMMIGVYIGQYCINALTYFFITWFP
                     VYLVQARGMSILKAGFVASVPAVCGFIGGVLGGIISDWLMRRTGSLNIARKTPIVMGM
                     LLSMVMVFCNYVNVEWMIIGFMALAFFGKGIGALGWAVMADTAPKEISGLSGGLFNMF
                     GNISGIVTPIAIGYIVGTTGSFNGALIYVGVHALIAVLSYLVLVGDIKRIELKPVAGQ
                     "
     gene            complement(52677..53126)
                     /locus_tag="J1Y83_000341"
     CDS             complement(52677..53126)
                     /locus_tag="J1Y83_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HBA7628528.1"
                     /translation="MAEIGIFVGTMYGNSLLVAEEAEAILTAQGHKATVFEDPELSDW
                     LPYQDKYVLVVTSTTGQGDLPDSIVPLFQGIKDSLGFQPNLRYGVIALGDSSYVNFCN
                     GGKQFDALLQEQSAQRVGEMLLIDASENPEPETESNPWVEHWGTMLS"
     gene            complement(53144..53926)
                     /gene="truC"
                     /locus_tag="J1Y83_000342"
     CDS             complement(53144..53926)
                     /gene="truC"
                     /locus_tag="J1Y83_000342"
                     /EC_number="5.4.99.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(65) synthase TruC"
                     /protein_id="HBA7628529.1"
                     /translation="MLEILYQDEWLVAVNKPSGWLVHRSWLDRDEKVVVMQTVRDQIG
                     QHVFTAHRLDRPTSGVLLMGLSSEAGRLLAQQFEQHQIQKRYHAIVRGWLMEEAVLDY
                     PLVEELDKIADKFAREDKGPQPAVTHYRGLATVEMPVATGRYPTTRYGLVELEPKTGR
                     KHQLRRHLAHLRHPIIGDSKHGDLRQNRSGAEHFGLQRLMLHASQLSLTHPFTGEPLA
                     IHAGLDDTWMQALSQFGWRGLLPENERVEFSAPSGQDGEISS"
     gene            complement(53926..54255)
                     /locus_tag="J1Y83_000343"
     CDS             complement(53926..54255)
                     /locus_tag="J1Y83_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqcC family protein"
                     /protein_id="HBA7628530.1"
                     /translation="MTTHDRVRLQLQALEALLREHQHWRNDEPLPHQFNSTQPFFMDT
                     MEPLEWLQWVLIPRMHDLLDNNQPLPGAFAVAPYYEMALATDHPQRALILAELEKLDA
                     LFADDAS"
BASE COUNT        13332 a        14420 c        13343 g        13598 t
ORIGIN      
        1 ctcaaaaaat cattcattag taaaaaggat atttatatgc aaaacatagc tgtggtttat
       61 tattcaggat atggtcacac taaactggtt gctgaaaaag tggcacaagg cgcccatgca
      121 gatttaattt cgattgataa cgaaggaaat atcactgagc aggagtggga aaaactggac
      181 gcagccgatg ccattatctt cggtgctccc acctatatgg gcggtgcgcc atggcaattc
      241 aagaaatttg ctgatgccag ttccaaagcg tggtttacac gtaaatggca ggataaagtc
      301 ttcgggggtt tcaccaacag cgccagcctg aatggcgata aacaagttac gctgatctat
      361 ctgcaaacgc tggcttcaca gcatggcggt ctgtgggtga gtttaggtca ggctcctgcc
      421 aatgtgcttg cgtcaaccag ggaagatgta aacaatctgg gcggttcagt cggtttgctg
      481 gtgcaatctc cagccgatgc gggtgcagac caaatcccta cgggcgatct ggatacagcc
      541 gtgaaatatg gtgaacgcgt ggcgacaatt actgtgcgct taaaatgatg attgccaaat
      601 gtgacgctga cgcgtctatc cggtcttcta catctaaaaa gtgccgtccc ctggtcagtc
      661 agataaagtt ttaacgctgt acccgataac agttcaacat gtaccatgta ccaaaagaaa
      721 aaaagccagc ctcgcttgag actggccttt ctgacagatg cttacttact cgcggaacag
      781 cgcttcgata ttcagcccct gcgtttgcag gatttcgcgc aaacggcgca ggccttcaac
      841 ctgaatctgg cgaacacgtt cacgggtgag tccaatttca cgacctacat cttccagtgt
      901 tgccgcttcg taccccagca aaccgaatcg acgtgccagt acttcacgct gtttggcgtt
      961 cagctcgaac agccatttga cgatgctctg cttcatatcg tcatcttgcg tggtatcttc
     1021 cggaccattc tctttttcat cggccaggat gtccagcaac gctttttcgg aatcaccacc
     1081 cagcggggtg tctaccgagg taatgcgctc gttaagacga agcatacggc tgacgtcatc
     1141 aactggctta tccagttgct ctgcgatctc ttccgcactt ggttcgtggt ccagcttatg
     1201 ggacaactca cgtgcggttc gcaggtaaac gttcagctcc tttacgatgt gaatcggcaa
     1261 acgaatagta cgggtttggt tcataatcgc ccgttcgatc gtctggcgaa tccaccaggt
     1321 tgcgtatgtt gagaagcgga aaccacgttc cgggtcaaac ttctctaccg cgcggatcag
     1381 ccccaggttg ccctcttcga tcaggtccag caacgccaga ccacgattgc cataacggcg
     1441 ggcaattttt accaccagac gcaagttact ctcgatcatc cggcggcgag aggcgacatc
     1501 tccacgcagt gcgcgacgcg caaaataaac ttcttcttcg gccgttaaca gtggtgaata
     1561 accaatctca ccaaggtaaa gctgagtcgc gtccagcaca cgctgtgtgg ctccctgcga
     1621 taacagttcc tcttcggcca aatcgttatc actgggttcc tcttctacta aggccttttc
     1681 gtcaaaaacc tcaactccgt tctcatcaaa ttccgcatct tcatttaaat catgaacttt
     1741 cagcgtattc tgactcataa ggtggctcct acccgtgatc ccttgacgga acattcaagc
     1801 aaaagcctgg ttccgccgat ttatcgctgc ggcaaataac gcagcgggtt tacggatttc
     1861 cccttgtaac gaatttcaaa atgcaagcgt gttgaactgg ttccggtgct acccatggtt
     1921 gctatttttt gccccgcctt cacttcttgt tgttcccgga ccagcattgt gtcgttatgg
     1981 gcgtaggcac tcaggtaatc atcattatgt ttgatgataa tcagattacc gtagccgcgc
     2041 agcgcgttac cggcataaac aacgcggcca tctgcggtcg cgataattgc ctgtcctttg
     2101 ctgcctgcga tatcaatccc cttgttgccc ccctcagaag cgccaaaggt ttcgatcact
     2161 ttgccctcag tcggccagcg ccaggtggag ataggcgtac tggttgatgt actgctgaca
     2221 gtcggctcgg ttgtgcttgc tgttggtacc gttacaggcg ctgtgaccgt ggtcgcagtt
     2281 ggcttgttgt tcggcaacat tttgttagca ctctgttcac ccgaagactc agaatacgta
     2341 attgtcggtt gcgacgcaac agcaacggtg gaattttgtg caggcttgat cacaactcct
     2401 tgctctgctg cgtcggcctg ggtaatggca tttccgcctg tgattggcgt accggaagca
     2461 ttacccacct gtaaggtctg accgacgttc agcgcgtatg gtgcctgaat attgttgcgc
     2521 tgagcaaggt cacggaaatc gttgccagta atccaggcga tataaaaaag cgtgtcgcct
     2581 tttttcacgg tgtatgtact gccgctataa ctgcctttcg gaatgttccc atactgacgg
     2641 ttgtagacga ttcgtccgtt ttccatctgt accggctgct gagctactgg ctgcacaggc
     2701 tggatttgcg gttgttgagt agcctgaatt tgtggctgct gtaccggctg aatttgcggt
     2761 tgctgcgctg tagacgtcgt ccccattttc ggcggcggcg taatcaacat accagaattg
     2821 gtatttgcgg gcgcattgcc attaacggag ctgaccggtg ccggtggatt tgaagtgtca
     2881 gaacagcctg ccagccatag cgaaaccagt gacaatgccg caatgcggcg aacggtgaat
     2941 tttgggcttc ccgcgctcat ttatccccca ggaaaaattg gttaataacc agtgacataa
     3001 ttaccgtgca aggcaccata ctgaacactg gaaaagatgt tcacgatacg ctgacctgcg
     3061 gcaaaataac caggaaaaat ccaggtattt cctcacgctt taagccagct cacccttcac
     3121 taaagggaca aagcgcacgg cctccacggt atcgataata aattcgcctc cccgacgacg
     3181 cacccgtttc aaatactggt gctcctcccc tacgggtaag acgagaatcc cgccttcgtc
     3241 cagctgcgtc attagcgcag ttggaatttc aggcggtgcc gccgtaacaa tgatagcgtc
     3301 aaacggcgcg cgtgcctgcc aaccttgcca tccatcgcca tgacgggttg aaacattatg
     3361 taaatcaaga tttttcaggc ggcgacgcgc ctgccactgc aagcctttaa tccgttcaac
     3421 cgagcaaaca tgttggacaa gatgcgccag gattgcagtt tgatatcccg aaccagtgcc
     3481 aatttccagc acccgcgact gcggcgtcag ctcgagtaat tcggtcattc gcgccaccat
     3541 atacggctgc gaaattgtct gcccctgacc tatcggcaag gcgatattgt cccaggcttt
     3601 ttgttcaaac gcttcatcaa cgaatttttc acgcggcacg gcggcaagtg cattcagcac
     3661 caactcatcc tgaatacctt gcgcacgtaa ttgatccaga agtgcttgta cgcgtctgct
     3721 taccattgcg tgccaactcc cacgctgttt aaccagtctg aaaccacatc ttgcgcgcta
     3781 tgcgcagtta aatccacatg cagcggcgtg atggaaacat agccctcatc taccgcagca
     3841 aaatcggtcc ccggaccggc atcacattta ccgcccggcg ggccaatcca gtacagcgta
     3901 ttgccgcgcg gatcttgctg cgggatcacc tgatctgccg gatgtcgtgt accgcagcgc
     3961 gtcacgcgaa tacctttgat ttgatccaat ggtaaatccg gaacgttaat attaagaata
     4021 cgcccggtgc gcagcggctc tttacacagt gcgcgcaaaa ttgagcaggt taccgccgcg
     4081 gcagtgtcgt aatgtttatg cccgtcaagc gagacggcaa gcgccggaaa acctaaatga
     4141 cggccttcca tcgcggccgc taccgtaccg gaataaataa catcatcccc cagattcggc
     4201 ccggcgttaa ttccggacac aacaatgtcc gggcgcgggc gcatcagagc atttacgcca
     4261 agatagacgc aatcggtcgg ggttcccatt tgcacagcaa tatcaccatt ttcaaaggta
     4321 aacgtgcgca gggaggattc cagtgtcaga gaattagaag cgccgctgcg gttacgatcg
     4381 ggggcgacca cctgaacgtc agcaaactca cgcaaggctt tcgccagcgt ttgtataccg
     4441 ggtgcatgta ccccatcatc attactcagc aatatgcgca taatcacctg ttgtgttgat
     4501 aagttccctg acaacgctgg ttgcaaaact tcccgccgga agccagaaat ggatttctac
     4561 ggtgacgtca tcccaccagt tccagcttaa ttgttgcgga tacagcagca tcgctctgcg
     4621 cgcggcttca actttttcgc gcaccagtaa ggtttgtaat tcagtttctt cggcgacagc
     4681 tgcttgttcg aatgccagcg cttcacgctg agttccccat tcgccactgc ccggcaatgc
     4741 ggcggttatc atcagctctt tatcgttgac gcgacgctgt aattccgcca gttcttcggt
     4801 ggttgcgaca aaccagctac cacgtccggc taattgtagc gcatcgccgt caacaacttg
     4861 attaacgtct gcttttttga ggcgctcagc aacaatctga ttaaacaacg cactgcgggc
     4921 tgccgacaac caaaaactcc gtttattgcg atcgcgcacc ggagtattgg tttgcgccca
     4981 gcgcagcgca ccctgcaagt tgctaccgcc aatcccaaaa cgttgggcac cgaagtagtt
     5041 cggtacacct tttacgcaaa tatcgatcag acgttgttca acgtcatcgc gattgctcac
     5101 ttcgcgtagt accagggtaa aggcgttacc tttcagcgcc cctaaacgca gcttgcgctt
     5161 gtgccgcgca tactccagca cctggcagcc ttccagttga aaggcgctca gatcgggcat
     5221 ctctttgccc ggcacgcgag cgcataacca ctgttccgta acagcatgtt tatctttttg
     5281 cccagcgaag ctgacttcac gggcatgaat tttcaggaat ttcgccagtg catccgccac
     5341 aaaacgggta ttgcagccgt ttttgaggat tctaaccaga atatgctcac cttcaccatc
     5401 aggctcaaag cccaaatctt ccaccaccac aaagtcttcc ggattggctt tcagcagccc
     5461 ggtgccttgc ggtttaccgt ggaggtaagt gagattatca aactcaatca ttttgttgcc
     5521 ttaatgagta gcgccaccgc ttcacaggca atcccttccc cacgtccggt aaatcccagt
     5581 ttttccgtag tagtggcttt cacgttaaca tcatccatat ggcagccgag atcttcggca
     5641 ataaacacgc gcatttgtgg aatgtgcggc aacatcctcg gtgcctgagc gatgatagtg
     5701 acatcgacgt tgccgagggc ataacctttc gcctgaatac gacgccaggc ttcgcgcaac
     5761 agctcgcggc tgtcggcacc tttaaatgcc ggatcggtat ccgggaacag cttgcctata
     5821 tctcccagcg ccgccgcgcc aagcaatgca tcggtcaacg catggagcgc cacgtcgcca
     5881 tcagaatgcg ccagcagccc tttttcgtaa ggaatgcgta cgccaccaat gataattggg
     5941 ccttcaccgc caaaggcgtg tacgtcaaaa ccgtgtccaa ttcgcattat gtattctcct
     6001 gatggatggt tcgggtgagg taaaattcgg ccagcgccaa atcctccggg cgcgttacct
     6061 tgatgttatc cgcgcggcct tcaacaagtt gcggatgaaa accgcaatat tccagcgccg
     6121 aggcctcatc ggtaatggta gcgccttcat ttagagcgcg cgtcaggcag tcatgtaaca
     6181 gctcacgagg gaaaaattgc ggcgtcagcg cgtgccataa gccgttgcga tcaacggtat
     6241 gagcaatggc atttttgccc ggttcggcac gtttcatcgt atcgcgcacc ggcgcagcga
     6301 ggatccctcc cgtgcggctg gtttcgctca acgccaacaa tcgcgcgagg tcatcctgat
     6361 gcagacaagg acgagcggcg tcatgcacca atacccactg cgcgtcgcca gcggctttca
     6421 gacctgccag cacggaatcg gcacgctctt caccaccatc aacaaccgtg attcgtggat
     6481 gattcgccag aggaagttgt gcaaagcggc tatcgccagg acttatggca atgacgacac
     6541 gtttcaccct gggatgcgcc agcagcgcat gcaccgagtg ttcaagaatg gtttgattac
     6601 cgattgagag atattgctta ggacattccg tttgcattcg gcggccaaat ccggccgccg
     6661 gaaccacggc gcaaacatcc aaatgagtgg ttgccatgtt aattcccggg ctgatttatc
     6721 gattgttttg ccccgcagac tgtgcgcgct ttgacgcgtc aggcaccaga cgataaaacg
     6781 tttcgcccgg cctggtcatg ctgagttcat tacgcgcacg ctcttcgagc gcctcctggc
     6841 cgccattgag atcgtcaatt tcggcaaaaa gctgatcgtt tcgcgcttta agtttcgcgt
     6901 ttgtagcttg ctgtgccgcc acatcattat tgacgcgagt atagtcatgt ataccgttct
     6961 taccgaacca cagcgaatac tgtagccaga ccagaatagc cagcaacagc agcgttagtt
     7021 tacccatcct gccccctgaa aaacggcatc ttcatcccat gcatccgaag acgactctac
     7081 atcctctgtt ggggataccg cgacaacgcg ggcaaatgta ccacatttgt ccattgttac
     7141 gtatacccag ggcgtgcaga acataatctc attattagtt acggattgaa ttatgaacag
     7201 aatagagagg aaagaacaat taacccagta gccacataaa cagcgcacca aacataatgc
     7261 caaccgtcgc cagagtgaaa acaatactgt agcgtagctt tccgtccatc aatgaatgca
     7321 gtgcaatccc caccaccact gcgacgggca tcagcgccag aaagaaaggc caggtgtaga
     7381 taaaaaagag cagcgtgtta gagccataaa tcaacatcgg aattgccaac gcgcacagcc
     7441 acgataaaaa accaaccaca gcgcctggca gcgaccatgt ggtgtcttca acctcagtaa
     7501 ggccgtcgtt atttgttaat gtaatgttgt ggctgttacg catatctgat cctgttactt
     7561 tgacgaaccg ggcatgaaaa cccggtggtg tctcaggatc tgataatatc gttctgtctc
     7621 aacagatcta ataattgctg taccaaattt gttactaatt gttcaccatt gagatgaatt
     7681 tctgcggatt caggcgcttc gtaaacggaa tctattcccg taaagttgcg cagttcaccg
     7741 gcacgcgctt tcttatataa accttttgga tcgcgggctt cgcaaatcgc cagcggcgta
     7801 tcgacaaaca cttcgataaa gcgcccttct cctacgcgtt cgcgaaccat ctggcgttcg
     7861 gcgcggtgtg gcgagataaa tgcggtcagc accaccagtc cggcttcaac catcaaattc
     7921 gccacttcac cgacgcgacg gatattctct ttacggtcgg catcgctaaa accgagatcg
     7981 ctgcataatc cgtggcgaac gttgtcgcca tccagcagat acgtactgac gccaagttta
     8041 tgtaacgcct cctccagcgc ccccgcgacg gttgatttac cggaaccaga gaggccggta
     8101 aaccacagca caacaccacg atgaccgtgg tgctgttcgc gttgttgcgg agttactggg
     8161 tggctatgcc agacgacgtt ttcgtcatgc agagccatta tttatctccc agtaaatcgc
     8221 gcgcacccca atgcgggaaa tggcggcgaa ccagggcatt caattccagt tcgaatgcac
     8281 tgaattcaga tggcgcagca gttgcctggc taactggttc gtgcaccata ccggcaccta
     8341 cggtcacatt actcaggcga tcgataaaaa tcagcccacc ggtaaccggg ttttgctgat
     8401 aacgatctaa caccagtggc tcgtcaaaag tgagatccac caggccgatg ccgttcagtg
     8461 gcaggttttc aacttcgcgt tgggtaaggt tattaatatc aacctgataa cgaatgccat
     8521 caacacgagc acgcgtcttc ttaccggcaa ttttgatgtc gtaactctgg cccggggaaa
     8581 gcggctgttc cgccatccat accacatcca acgacgcgct ctgcaccgcc ggtaacgctt
     8641 cgtctgccgc caacagcaga tcgccacggc tgatgtcgat ctcatccgtc agcaccaggg
     8701 tgatcgcttc tccggcaaag gcttcttcgc gatcaccatc aaaagtcacg atccgcgcga
     8761 cgtttgattc cacaccagag ggcagcactt ttacacgttg cccgacttcc acgcgaccgg
     8821 atgccagcgt tccggcgtaa ccacgaaaat cgagattcgg gcggttaaca tactgcaccg
     8881 ggaagcgcat tggctgggca tccaccactc gctggatctc cacggtttcc agcacttcga
     8941 gcagtgtcgg accgctgtac cacggcatac tttcactttg cgaagccaca ttgtcgcctt
     9001 ccagtgcaga gagcggcaca aagcggatat ccagattacc cggcagttgc ccggcaaagg
     9061 tcaaataatc ttcacgaata cgggtgaacg tctcttcact gtaatccacc agatccattt
     9121 tgttgatcgc cacgaccaga tgtttgatcc ccaacagtgt ggagataaaa ctgtgacgac
     9181 gggtttgatc gagcacgcct ttacgggcat cgatcagtaa gatcgccagt tcacatgtcg
     9241 atgcgccagt cgccatattg cgggtgtact gctcgtgccc tggggtgtcg gcgataataa
     9301 atttacgctt ctcggtagag aaatagcggt aggccacatc aatagtgatg ccctgttcgc
     9361 gctcagcttg caggccgtcc accagcagtg ccagatccag cttttcgccc tgggtgccgt
     9421 gacgcttact gtcgttatgc agcgatgaga gctgatcttc gtagatttgg cgggtatcgt
     9481 gcagcaggcg accaatcagg gtacttttgc cgtcatcgac gctaccacag gtcagaaaac
     9541 gcagcaggct tttatgttgt tgcgcaatca tccaggcttc gacgccgcct tcattggcga
     9601 tttgttgtgc aagtgcggtg ttcatcttaa aaatacccct gacgtttttt cagctccata
     9661 gaccccgcct ggtcgcggtc aattacgcgg ccctgacgtt cactggtggt ggaaaccagc
     9721 atctcttcga tgatctccgg cagtgtttgt gcgtttgact ccaccgcgcc ggtcagcggc
     9781 cagcagccca gcgtacggaa acgcaccatc cgttttttaa tcacttcgcc cggttgcagg
     9841 tcgatacggt tgtcatcaat catcatcaac ataccgtcgc gttccagaac cggacgttcc
     9901 gcagcgagat acagcggaac aatgtcgata ttttccagcc agatgtattg ccagatatcc
     9961 tgctcggtcc agttagagag cgggaagacg cggatgcttt cgcctttgtt aatttgcccg
    10021 ttgtagttgt gccacagctc cgggcgctga tttttcggat cccagcgatg gaagcggtca
    10081 cggaaagagt agattcgctc tttagcgcgg gatttctctt cgtcacggcg cgcaccaccg
    10141 aaggcggcat caaaaccgta tttgttcagc gcctgtttca ggccttcggt tttcataata
    10201 tcggtatgtt tcgcgctgcc gtgtacgaat ggattaatcc ccatcgccac gccttccggg
    10261 tttttatgca ccagcagctc gcagccgtag gctttcgccg tacgatcgcg gaactcatac
    10321 atctcgcgga atttccagcc ggtatcgaca tgcagcaacg ggaaaggcag cgtacctgga
    10381 taaaacgcct tgcgcgccag atgcagcatg acgctggaat ctttaccgat agagtagagc
    10441 atcaccggat ttgagaattc tgcagctacc tcgcgaataa tgtggatgct ttccgcctcc
    10501 agttgccgca ggtgggtaag tcgtttttga tccataaccg ttcctttgca ataccgctat
    10561 tttcttgcca tcagatgttt cgactatagg gagcgtaaga gaacgaatga aattaccaat
    10621 tagaatgagt agttccttaa cggaataacg atttggcaaa gctaatatca aaaagtgctt
    10681 aaggcaccgg atttcgggcg tttaggaaga tttgaaattg ttttagcgca gcggcagttt
    10741 catactatgg cggtaaaaaa atttgcatgg tatttaagga ctcactatgt tttccgcatt
    10801 gcgccaccgt accgctgccc tggcgctcgg cgtatgcttt attctccccg tacacgcctc
    10861 gtcacctaaa cctggcgatt ttgctaatac ccaggcacga catattgcta ctttctttcc
    10921 gggacgcatg accggaactc ctgcagaaat gttatctgcc gattatattc gccaacagtt
    10981 tcagcaaatg ggttatcgca gtgatattcg gacatttaat agtcggtata tttataccgc
    11041 ccgcgataat cgcaagagct ggcataacgt gacgggaagt acggtgattg ccgctcatga
    11101 aggcaaagcg ccgcagcaga tcatcattat ggcgcatctg gatacttacg ccccgctgag
    11161 cgatgctgac gccgatgcca atctcggcgg gctaacgtta caaggaatgg atgataacgc
    11221 cgcaggttta ggtgtcatgc tggaactggc tgaacgcctg aaaaatacgc ctaccgagta
    11281 tggtattcga tttgtggcga ccagcggcga agaggaagga aaattaggcg ctgagaattt
    11341 actcaagcgg atgagtgaca ccgaaaagaa aaatacgctg ctggtgatta atctcgataa
    11401 cttaattgtt ggcgataagc tgtatttcaa cagtggcgta aaaacacctg aagcagtaag
    11461 gaaattaacg cgcgaccggg cgctggcaat tgcccgcagc catggaattg ccgccaccac
    11521 aaatccaggt ttgaataaaa actatccgaa aggcaccggg tgttgtaatg acgcagaaat
    11581 attcgacaaa gccggtatta ctgtactttc ggtggaagcg actaactgga atcttggcaa
    11641 taaggatggt tatcagcaac gcgcaaaaac agccgcattc cctgcgggaa atagctggca
    11701 tgacgtaaga ctggataatc aacagcatat tgataaggca cttcctggaa gaatagaacg
    11761 tcgctgccgt gatgttatgc ggataatgct accgttggtg aaggagctgg cgaaggcatc
    11821 ttaatgggtt tgaaatgatt actcccttgc ctgcaggaaa tcaatgccac cagaaacaga
    11881 gtataaaaaa gggtgcagga gaccaaacca tcacaattca gtacgcgggg ttttaccccg
    11941 cgtggtggct acttcttcga ttcgtaagcg agaactgcct taaactccat accacgttct
    12001 ctgatactca actcacacaa cgagtcgcct accatcaacg tgaatcccag tactgtacaa
    12061 cacagtacga tgattgccac aagggcatat tttgtcagca tgtcttgcct ccctgcaaag
    12121 aagagactaa catccgaatt gcttaggttc gaacgtaggc ctcaggttga tagaaatatc
    12181 gcctggggct tttgtccatc tggaacctcg cgaatgctta acgccagaca gcctcaagca
    12241 cccaatgcca ttctatacct gttaattctc cctgcgacgt tctcgtttaa atgccggaat
    12301 gctgctcgca aaataaaaac gcccccatca tttctgacag aggcgtttaa tttatccggc
    12361 aatatttacc cttcgtgtaa tccacattcc ctttttaagc caaagaaacg cgtttcttct
    12421 tccgccatgc cgggttccca tttacgggtt gtatgggtat cgcccaccga taaatatcct
    12481 tcatcccata atgggtgata tttcaggcca tgtttttgca ggtactgata aatagttcgg
    12541 ttatcccagt cgataatcgg cagcacttta aatacgccac gctgaattgc cagcaccggt
    12601 aaattggcgc ggctgccgga ttgttcacgg cgcaggccag caaaccaggt ttgcacattc
    12661 agttctttca gcgcccggtt catcggttcg actttgttga tgtcattgta cttttcaatg
    12721 ccttcaacgc cctgctccca cagtttgccg tagcgtgctt cctgccaggc ggcgctttcg
    12781 gtagcacggt acactttcag gttgagcttg agtttatccg ttaactcgtc aataaagcgg
    12841 taggtttccg ggaacagata gccggtatcg gtgaggatca ctggaatatc cgggcgaatc
    12901 tgattcacca gatgcagact caccgccgcc tgaatgccaa agctggaaga aagtacatat
    12961 tcaccgggca gattatccag cgcccaggct acgcggcctt cagcgtccag tttttccagt
    13021 tgggcgttag tttccgccag cgccagaatc cggtcgacct ttggcagttc gttcagggcg
    13081 tttagatcga gtttggacat aagttcctca ctgtttgcct tgcctgatgc aacgcttaca
    13141 catcttatca ggcctacaag accgggctga tggttaatcc cacaaatcgc gcgccggatc
    13201 gagcaccggg cgaatgatgc ccgcacgcac cgtaaagtcg ccgaagcctt cacccacttc
    13261 gcgctctttc gcccagcgcc ctatcagttc atcaagcgac gccaggattt ccggctcggt
    13321 gatgttttct ttatacatcc gtgggatacg tgtcccaatg cggttgccgc caagatgcag
    13381 gttgtagcga cccggcgctt tccccaccag acccacttcc gccagcatcg cgcgaccaca
    13441 accgttcggg cagcctgtta cacgcatcac gatatgctca tcgctgacac catgtttcgc
    13501 cattaaatta tcgatgttgt cgataaaaga cggcaggaaa cgctccgctt ccgccatcgc
    13561 cagcgggcaa gtcgggaatg acacgcaggc cattgagttt tcacgctgcg gcgtgacggc
    13621 attcattaac ccgctttctt tggcgatctt ctcgatcttc gctttctcgc tttccggcac
    13681 gccggcgatg atcagattct ggttcgccgt aatgcggaaa tcacctttgt ggatcttcgc
    13741 gatctccagc aagccggttt tcagcggacg tcccggataa tcaagaatgc gaccattttc
    13801 gataaacagc gtcaggtgcc agttatcatc aatgccctta acccagccaa tacgatcgcc
    13861 gcgtccggta aactcatacg ggcgaatcgg ctcgaatttg atccccgcgc gacgttcgac
    13921 ttccgcttta aatgtctcaa cccccacccg ctccagcgtg tatttggttt tggcattttt
    13981 acgatcggta cgattacccc agtcgcgctg agtcgtcaca acggcttccg ctaccgccag
    14041 cgtatgttcc agcggtaaat aaccaaactc actcgccgtg cgggcgtagg ttttcttgtt
    14101 gccgtgttcg atggaaagcc caccgccgac cagcaggtta aagcccacca gcttgccgtt
    14161 ttcggcgatc gccacgaagt tcatgtcgtt ggcgtgcaga tcgatatcgt tctgcggcgg
    14221 gatcactacc gtggttttga acttacgcgg caggtaggcc tgaccaagga tcggctcttc
    14281 atcagtagtg gcgacttttt cctgatcgag ccagatctcc gcatacgcgc gggtacgagg
    14341 cagcagatgt tcagaaattt tcttcgccca ttcatatgct tccgcgtgca gctgcgactc
    14401 gtaagggttc gaggtgcaga gtacgttacg gttcatgtcg ttggcggtcg ccagcgcatc
    14461 aagaccgacc gagtgcagca tctggtgcac cggtttgacg ttctttttca gaatgccgtg
    14521 gaactgaaac gtctggcggt tggtcaggcg aatgctgcca tagatggtgt tttcaccggc
    14581 aaatttgtcg atcgcctgcc actgtttggt ggtgatcacg ccacccggca gacggcagcg
    14641 caacagcatc gcgtggcgcg gctccagctt ctgttcagca cgttcggcgc ggatgtcgcg
    14701 gtcatcctgc tgatacatgc cgtggaagcg gatcagcagg aagttgtcgc ccttaaagcc
    14761 gccggtcaga ccgtcgttta aatcttccgc aatggtgccg cgcaggtagt tgctttcaag
    14821 cttcatgcgc tcggcgtctg tcagttttcc ttcgaccact aaaggccctg gatgtttttc
    14881 gctcattagt agacatctcg ctgataacgg cgctctacgc gcagctcact taaaaattca
    14941 tccgccgctt cggtgtccat gccaccaaat tcggcaatca cttccagaag tgcctgctca
    15001 acgtctttcg ccatgcgatt agcgtcgccg cagacataaa tgtgggcacc gtcattgatc
    15061 cagcgccaca gctctgcgcc ctgttcgcgc agtttgtctt gtacgtaaat tttttctttt
    15121 tgatcgcgcg accaggcgag atcgatgcgc gtcagcacgc cttctttgac gtaacgctgc
    15181 cattccacct ggtagaggaa atcttccgta aagtgtgggt tgccaaagaa tagccagttt
    15241 ttacccggcg cttcgtcggc agcacgctgc tgcataaagg cacggaacgg cgcgatgccg
    15301 gtgccagggc caatcatgat caccggggtt tccggattgg ttggcaggcg gaagttgtcg
    15361 ttatgttcga taaagacgcg gacttcaccc tcttcttcca cacgatccgc gaggaagctg
    15421 gaggcaccac cggcacgggc gcggccttcc acgtcgtaac gcaccacacc aacggtaacg
    15481 tgcacttcgt tctcgacttc cgcctgcgag gaggcgatgg aatacaggcg cggggtcagc
    15541 gggcgcagca gattaatcag cgcttcggca tccagttgcg ccggagagaa acgcaccatg
    15601 tcgacaatcg gcgtcgtcgc ggcgtaatgc tgtaacttcg ctttatcgcc caccagcggc
    15661 agcagcgttt cgctgcgggt aagcgtggca tagttctcaa caatgttggc ggtgttgacg
    15721 gtcagttcga agtgccactg tagcgcttcg ttcagaggca acgttttgcc ctcgacggtg
    15781 acaggttcat cgcctttcag ccacagcagt tcgacaagtt ctttcaccag tgccggatcg
    15841 ttttgatacc agacgcccag cgcgtcaccc ggctggtaac gcaggccaga gtcacctaag
    15901 tcaatttcaa tgtgacgaac gtctttttca gagttacgcc cggtaatttt ctggttaacc
    15961 gaaaggctgg ccgccagcgg cgcgtctttg ctatacgggc tggtgtggat ttcatttacc
    16021 gcgccagtag cgacggattg cgaaggcgcc gcgacaggcg cacgcgattt aagcgcatca
    16081 accacgcggg cgcgccactc gctggcagca gcctggtatt caacgtcggc atcgacacgg
    16141 tcgagcaggc gttcaccgcc cagttccgcc agcttactgt caaaatcttt cccggactgg
    16201 cagaaaaatt cataagagct atcgccgagg ctaaacacgg caaacgcggt gttttccagc
    16261 tttggcgctt ttttggagaa caggaactta tgcagcgcga cggcttcttc cggcggttcc
    16321 ccttcccctt gcgttgacgt caccacgata agcagttttt cgctggcgat ttgtttgaat
    16381 ttatagtcac ccgcgttcac cagcttaacg ttcagttttg ccgctaataa atcatcgcgt
    16441 aatgcttcag caacccgacg cgcattgcca gtttgcgagg cggagataat agttataccc
    16501 ggcatttctg cggcgggcgc tggcgtcgct gcaagcgcag caggctgctg gttgagcacg
    16561 ccccagaaat agccagaaac ccaggcaagc tgggtaggag ttaaatcggt cgtggccgcc
    16621 tgaaggcgtg ccagttgctc cgggttcaac ggaagcaacg cggaaggtgg gacctgtgtc
    16681 gtcatgcgtt gttatgttcc agtaagcaaa gctgtttctg cgccctgtca gcgcccataa
    16741 aacagaagag aaggtaaggt taacggggta aacggtgtgg attaaagacg ggatagcgat
    16801 aactaataac caaatcgact aacctgtttt agcaatagtc tttaacaaca aaatagatta
    16861 accaacccaa tgaaaaacaa atgaatattg ccaatcaata agataaatca gcgattttgc
    16921 gcaacaaagt cgttttagat aatgcgaaaa aacagccttt ccggtactct acggcggttt
    16981 tatcgtcatg tagagaaatt atgatgtcca ccacgttatt taaagatttc accttcgaag
    17041 ccgctcaccg cttgccgcac gtcccacaag ggcataaatg tggtcgcctg cacgggcatt
    17101 cctttatggt gcgactggaa attaccgggg aagtcgatcc gcatacgggc tggattatcg
    17161 atttcgctga actgaaagcg gcgtttaaac caacttatga gcgcctcgat caccattatc
    17221 tcaatgatat tcctggtctg gaaaacccaa ccagcgaagt tttagcaaaa tggatttggg
    17281 atcaggttaa acccgttgtg ccgctgttaa gtgcggtgat ggtaaaagaa acctgcaccg
    17341 caggttgtat ctatcgcggc gaatgataag agtgtgtcgg cggtcaattt cccttaagta
    17401 acgctatgtt agagtgttgt gttctggata tctggggcat gacatggaag acgactgcga
    17461 cattattatt attggtgccg gtattgcagg caccgcttgc gcgttacgct gcgcgcgagc
    17521 gggtttatcc gttttgttac tggaacgagc ggagattccc ggcagcaaaa acctttccgg
    17581 cgggcggcta tacacccatg cgctcgcgga actcctcccg caatttcacc tgaccgcgcc
    17641 tcttgaacga cgcatcactc acgaaagtct ttctctgtta acgccagatg gcgcaacgac
    17701 gttttccagc ttactgcccg gcggtgaatc ctggagtgta ttacgcgcac gattcgatcc
    17761 gtggctagtt gccgaagccg aaaaagaagg tgtcgaatgc atccccggtg cgacggtaga
    17821 tgcactgtat gaagaaaacg gcagggtgtg tggtgtcatt tgtggtgacg atattcttcg
    17881 cgcccgttat gtggtgctgg cagaaggtgc caacagcgtc ctggcggaac gccacgggtt
    17941 agtgactcgt cctgctggcg aagcgatggc attggggatc aaagaagcgc tggcgctgga
    18001 accatccgct attgaagaac gttttcatct ggagaataac gaaggcgcag cgttgctgtt
    18061 cagcggcgga atctgtgatg acttacccgg cggcgcattt ctttatacta atcaacaaac
    18121 gctctcgtta gggattgttt gcccgctctc ctcccttacg caaagtcgtg ttccggcaag
    18181 cgagctgctg gctcgcttta aaacgcatcc ggcagtgcgc ccgcttatca aaaacacgga
    18241 atcactggag tatggtgcgc atctggtgcc agaaggtggc ctgcgcagta tgccggtgca
    18301 atacgccggt aacggctggc tgctggtggg cgatgcgttg cgcagttgcg tcaataccgg
    18361 aatttccgtg cgcggtatgg atatggcgct aactggcgcg caggcggcgg cacaaacgct
    18421 gataagcgcc tgccagcacc gcgagccgca aaatctgttt ccgctttatc atcacaacgt
    18481 agagcgcagc ctgctgtggg atgttctaca gcgttatcag catgttccgg cgcttttgca
    18541 acgccctggc tggtatcggg tgtggcctgg gttaatgcag gatatttccc gtgatttatg
    18601 ggatcagggg aatacacctg ttccaccgct gcgccaattg ctctggcgtc atttacgtcg
    18661 tcacggcttg tggcatctga cgggcgatgt tatcaggagt ctgcgatgtc tgtagcccgt
    18721 aatctctggc gcgctgccga tgcgccgcac attgttccgg ctgactccgt tgagcgccag
    18781 acggcacaac ggttgattaa cgcctgtcca gcaggtcttt tttcgctcac acctgaaggt
    18841 gacttacgcg ttgattatca cggctgcctg gagtgtggca cctgccgttt gctgtgcgac
    18901 gaatcaacac tacaacagtg gcgctatccg ccttccggat tcggcatcac ctaccgcttt
    18961 ggataaataa taaggataat ttatgcccct cttacacctg ctccgccaga atccggtgat
    19021 tgctgccgtt aaagataatg ccagcctgca actggcaatc gactctgaat gccagtttat
    19081 ttccgtgttg tacggcaata tctgcaccat tagtaatatc gttaaaaaga ttaagaacgc
    19141 cgggaaatat gcttttattc atgttgattt gctggaaggc gcgtcaaata aagaagtggt
    19201 gattcagttt ttgaaactgg tgaccgaggc ggacggaatt atcagtacca aagcctcaat
    19261 gctgaaagca gcaagagcgg aaggtttttt ctgtattcat cgactgttta ttgttgattc
    19321 gatttcgttt cacaacattg ataagcaagt tgcgcaatcg aatccggatt gtattgagat
    19381 cctgccaggc tgcatgccta aagtgctggg ctgggtgacg gagaaaatcc gccaaccgct
    19441 gattgctggc gggctggtgt gcgatgaaga agatgcgcgt aatgcgatta acgcgggtgt
    19501 tgtggcgctt tccaccacga ataccggggt ctggacgtta gcgaaaaaat tgctttgacg
    19561 ggataatcgt aacaagttga atttggtgtg atttgtaggc tgcacgccac atccgacatt
    19621 cagcgcctga tacgacgctt gacgcgtctt atcaggctta caagtcccca gcccagaacc
    19681 gtaggcagga tcatccggca gcacgttatt ggcaatctgc gtgaatggtt gtcaccagtg
    19741 cctccagcac cactttccag tcatcaacca cgccaacatc tgcctgtgaa aataccgctg
    19801 cactggcgtc atgattaatc gccaccacaa acttgctatt gcgtacgccc gccattaacg
    19861 ctgctgcgcc ggaagcgcca acaacaatac acacctcagg tgccagcaga tgtccggaaa
    19921 taccaatcac tttttcggca tcaacgccac cgttcatcac tcgtgcccga ctgtagccca
    19981 cttcggctcc cagcttttct gccagcatag cgatttcctg gttatctgcc tctccgccct
    20041 gccccaccac cagcacccgt ctggcctcag ctaatgggtc acgggtaaca tttttcagat
    20101 tttcgacgct gataagccag tctggaagcg cgccggggac aatgttcagt tgctgcatcc
    20161 cagagggcaa cgtggcgttt ttatctgctc ccgcctgacg tgccagcgaa aggcatagcg
    20221 ggcgtttttc tgtttgcagc gtcgccgtta gcgcatttcc ccagtgggat ttgcgcacgc
    20281 ttacggttgg aatatctaac gaagtcacct ggcagatgct tgcaccatgt aaacgccagg
    20341 ccagccgggt cgacagttca tcgccaaacg ttcccggcgg aaagagaaca acgtccgcag
    20401 gcgttcgttg ccactgttcc accagcgcat ccagcacctg ttccgcaatc atcggctgtg
    20461 gttctatttg ccagtgtgcc agggtacaac cgctgaaatc ctgcgccgcc agccagctcg
    20521 ctattgcggc atggtcctga tttattgtca ccagtacgat attcatggct gcatcctttg
    20581 gcataaatag tcctgccata gcttttgcgc tttttctgct accgtttgcc catcaatcag
    20641 cgtcgctccg cgtcgttgct ctggccttgc cagttgcagg cactgtatcg ctggcgcttc
    20701 cgcggcaaca gtttcgcggg taatctccgc tttccccgcc gccattcgct ggcgcattcc
    20761 cggtactggc aaggccactt caccacactg acgcacggca atcaccgcag gcaagcgaac
    20821 ccggcaacag cgcaccccat gttcagtacg ctgttcgagg gtgataaacg gtgcgtcgag
    20881 agtaaagcgc tccacttggg tgaagcaggg ccagaccagc atttccgcca gcaaaaatgg
    20941 cgtttgccca ttctgccctt cgctgctttg gcagccagta attatcagat ccagcggatt
    21001 ctggtactgc cattcggcaa tatggcgagc gacaaattcc ggcgcaaagc gcagatctgc
    21061 cgccgtctcc agcaatacag cgtcctcaaa cccaagagcc atgagatagc gcagccagtg
    21121 caacgcccgt tcatccccca tgctcaacgc ggttaaagac atcggcgtac cgttttttct
    21181 ctgcgccaac agcagcgcgg cagcggcctg ttcatcagca ccgagtaaac ttcgcagtag
    21241 cgaaacatcc ggtccgctat taccctgagc cgccgccagc cactcttttt ccgccagcat
    21301 cccggcatcc ggttcggctt taaacgctaa cagaatgttc attcgcacct cctacatttc
    21361 ccgccgccac cagcgggagt gctttggttt ctggtgccca tagccaggtc acaacaaaac
    21421 caaccaacaa cactgccgcc aggagcaata aggtggcttg cattccccac tgcgccagca
    21481 cccacggcag caggccagta cttaccgccg cccccaaccg actcatggcg gtggcaaaac
    21541 cgacgcccag cgagcgaatg tcagtaggaa aactttccgc aggcaaaatg cccaccagat
    21601 tactgactgc cgaaatggtg gtgctgaaga gaacaaaaag cagcagcgtt aatgaactgc
    21661 cggaaggcaa acaggccatc actaccagcg ttgccgccag cagcaaaaaa cttcccagca
    21721 gaaatttgcg atgtgccagc aggtgcgtca gaactaatcc cagcagcgcg ccaacaatta
    21781 acaacgcatt aagcatcagg ctggcagtca gcgcatcttc cagtccaata gtctgggcga
    21841 tagttggcag ccaggtataa atcacaaacc atgggattac gaggcagaca aagaagacgc
    21901 tgttaaacgc tgtgcgccgc cagtagcgcg aagagaacaa ggttttgatg tgtttatggg
    21961 tcgccgttgc cacttcatcg cccagtaaaa catgcgagcc aaaatagcga tgaacgatag
    22021 cgtgagcttc tgcaaaacgc ccctggcgca gtagccagcg tggcgattct ggcgttcccc
    22081 agcgtaataa cgtaatcaac aacgcgggca gagctgccga tgccagcagc cagcgccagg
    22141 cctccgggct ttcgctaata aagtgatgtc cggcaatact tgccaacaca tagcctacgg
    22201 tccacaccac gctgaacgcg cccagcaaaa caccgcgatg gcggcgcgga gaaaattcag
    22261 ccagcaaagt gtgacctact gagtaatcgc ctcccagacc aatgccgatc aaaatgcgca
    22321 atccaataag atgctctggc gtggtggcaa aaaattgcaa gaacgaagcg agcgtaatca
    22381 gcataaaact gaaggtgaag attttttgcc gtccgatatg gtcggagatc caccccaaaa
    22441 ccaggctgcc aagaaacaaa ccaagtaacg ccgagccgcc gatcatcccc gccataaacg
    22501 gcgtcagttg catggcgggc gtaagctgaa taatggcgta accaataacg ccgaggacat
    22561 aaccgtcggt caggtgtgcg ccgaaagtga gcgcagcaat tcgacagtga aaacggttaa
    22621 gcggtaaatc atccattcgc accggtgaag tgttcatctt taccccttca ttgtccggta
    22681 ataagaacgg cttctcgcct gagaagccga aaaggcaacg ccccgtcatc gcggaacgct
    22741 gcctgacaca ttatttttca atcggataga tagtacccgt gttcataatg ccattaggat
    22801 cgaactgctt tttcagccct tccagcaacg cccacgcgct gccgtgttcc agtttgctcc
    22861 agtgaacgcg atgtttaccg ataccgtggt ggtgcaccat cgaaccgccg agacgaatgg
    22921 tttcttcgca gatgattttg ttgagcggat tgtggtactt gtcgatttcc tcttccggct
    22981 tacagtcaac gacgttgtag tcgtagacga agtacatgtt ggtgccgttc tggtagctgt
    23041 gagaggagtg accgcccagc atggtgatgt catcggcgta cgggaactcg gtacgaatac
    23101 ggttaataac gctttcgtag atttcgtgaa tgcagctcca gcagccggac acttcagtgg
    23161 taaagcccat gttgccggtt ttgaggatct gcacacgttc ggcagccact ttatccggtc
    23221 cccagttcag gttgttaaac caggtttcga tcagcttgct gtccacgcgc tggcattgcg
    23281 ggtaacgagc cacgatttca gcaatccctt cgcccgtcgc cttcgcaatg cgagggttac
    23341 cttcagccat aaagatcagc acgcatttgc cgtcggcaaa atgggtgaag tgttgggtgc
    23401 catcttcagc atcatacaaa cgagcaatcg acggacgata cccttccacc atgatttcac
    23461 gcaggatgtt gaagccggtt ttcatgtctt ccagcacata gccatagaag agattgtttt
    23521 ccggcgtaaa tttgaagatt ttcactgtga cttcagtgat atagcacagt gcaccttcgt
    23581 tgccgatgat gatatgacga atgtccgggc cagccgcgcg acgtggcacg tttttaatgc
    23641 gtgtgacggt gccatctgcc agcaccgctt ccagaccaac gaccatatcc tcgattgcgc
    23701 cgtagagtgt ggaaaactgc ccgatactgc gggtcgccac caggccgccc atctgcgcca
    23761 gcggctttga ctgcggagaa tgccccgtgg tgtaaccttt ttcacgcaac gcgttttcca
    23821 gcacctccag cggaacgcca cattgcgccg tggcctgcat attctcaata tcaatattaa
    23881 tgatttgatt catggcggag ccgtcgagca ccaccgagtt ttccacaacg gtttccagcc
    23941 caccttcggt ggcggaagca ccggtacgtg gcacaccgtt aattttgtgc gcattcataa
    24001 aattcagcac acgggatact tgttctgtag aaccgagttt tacgaccgct gccggaatcg
    24061 gcaaagtata aattccatga atatccggaa atttacgaaa acggtcaata ctgtttttct
    24121 ttaataccgt ttcatcggta attacgcgat ctgcaccaac aatttccttt agctggtcga
    24181 caatcgctac gcgagataaa gacatagtaa ttccttcctg ataataaaaa tggaatagtt
    24241 attaataaga gaaaacagaa accaccgtta tgaattagcg cactaaataa ccgccatcaa
    24301 ccactaataa atgtccgttg acataattcg atgccggact tgcgaggaat acggctgcac
    24361 ccattaaatc ctgagtatca ccccaacggt ttgccggaat atgatcaaga acacgctggt
    24421 tagtttctgg attactacgg gtcgccagcg taatatcggt tgcataataa ccaggagcaa
    24481 taccattcac ctgaatatta tattggccta attcatcgca ataggctttg gtgaatccgg
    24541 caagagcatg tttagtggca gaataagcag gtgaccattg accacctaag taagagaaca
    24601 atgaacagat attaatgatt ttaccgcttt tctgcgggat cataattttt gccgcttcgt
    24661 agcttaactc gaatgcggcg gtcaggttca cgtcaatcat cgggtcccag tcggtacgac
    24721 caaagtccag caccttattc agcttacaaa taccggcatt gttaaccaga atatctacgg
    24781 taccgaagcg ctcacagcag gcagcgataa tcttctgcgg cgcgccttct gcggtgatat
    24841 ccacctgcat aaaatcaact tcgacaccct gtttttcaat catttcctta gtttcgccgt
    24901 tatctttgac gaaactagga ataaagacat ttgcgccagc tttggccaac gccatggcaa
    24961 atgcctggcc taaaccgcta ttcccgccgg taacaattgc tgttttacct ttcagggaga
    25021 aaaaatccat tgagaacgca ttgagagatt cgattgacat agttagctcc aatttccgta
    25081 aggcaaaaaa aaagaaaggt aacttccccc gcgagaggga agttaccttt ctcattatct
    25141 ctggtaactg gagataattt ttagcataag gacataactg ctaatgtgat cgtaatcaca
    25201 gtgtgatgtt cgtattgtca aataatttaa aaaagcagaa gcgtgattac tctcacacaa
    25261 cacctctgct ttccatgtta tttcttattg caattactag agacctgaga aaagtaatcc
    25321 acctgaaagg gcggcttact tttctctttt ttttgtctat tacaaatggg aatacgatat
    25381 gcaacacaac tcatatcgcc gttggataac cctcgcgata attagtttta gcggcggcgt
    25441 tagtttcgac ctggcttatt tacgttatat ttatcaaatt cccatggcaa aatttatggg
    25501 attcagcaat accgagatag gtttaataat gagtaccttt ggtattgcgg ccattatttt
    25561 ctatgcccct agcggcgtta ttgccgataa attttcacat cgcaaaatga ttacttccgc
    25621 gatgatcatt accggattac tgggtctgtt aatggcaaca tatccaccgc tgtgggtaat
    25681 gctctgtatt caggtcgcct ttgcgataac gacgatttta atgctgtggt cggtgtcgat
    25741 taaagccgca tcgttgctgg gcgatcatag cgagcaaggg aaaattatgg gctggatgga
    25801 agggctgcgc ggcgtcggtg taatgtcgct ggcggtgttt accatgtggg tcttttctcg
    25861 ctttgcaccg gatgacagcg ccagcctgaa aacggtcatt attatctata gtgtggttta
    25921 cattctgctg ggcattttgt gttggttttt tgttagcgat aacaacaatc tgcgcagtgc
    25981 caataacgaa gaaaaacagt cattccagct tagcgacatc ctggccgttt tgcgcatcag
    26041 caccacctgg tattgcagca tggtgatttt tggtgtcttc accatctacg ccattctgag
    26101 ttactccacc aactatctga ccgaaatgta cggcatgtcg ttggtggcgg caagctacat
    26161 ggggattgtg atcaacaaaa tcttccgcgc gctgtgcggc ccacttggcg gcattatcac
    26221 cacctacagt aaagtgaaat cccctacccg cgtggtccaa atcctttcca tcatcggttt
    26281 gctggcgttg acagccctgc tcgtcacgaa ctctaacccg caatcggtcg cgatggggat
    26341 tggcctgatt ttattactgg gattcacctg ttacgcctca cgcgggctgt actgggcctg
    26401 tcctggcgaa gcgagaacac cgtcttacat tatgggcacc acggtaggta tttgttcggt
    26461 gattggattt ctgccggatg tcttcgttta cccgattatc ggctactggc aagacaccct
    26521 gcccgctgca gaagcctacc gcaacatgta gctaatgggc atggcggcgc ttggcatggt
    26581 gatcatcttt acctttttgc tgttccaaaa aattcgtact gctgatagcg cccccgcaat
    26641 ggctagcagc aagtaagcca ggaaaattac aggagaaaaa tcatgtcgaa aaaatacatc
    26701 atagggattg atggcggaag tcagagcaca aaagtggtga tgtacgatct ggaaggtaat
    26761 gtggtttgcg aaggcaaagg cttattgcag ccgatgcaca cgccagatgc cgataccgca
    26821 gaacatcctg acgacgattt atgggcatca ctatgttttg ccagtcacga tttgatgagc
    26881 cagtttgccg gaaataaaga agatattgtc ggcattggcc tgggctcaat ccgttgctgc
    26941 cgtgcgttat tgaaagccga tggtacgcca gccgcgccgt tgattagctg gcaggatgca
    27001 cgcgttacac gcccttacga acacactaat cctgacgtgg cctatgtcac ctctttttcg
    27061 ggttatctga cacatcgctt aaccggcgag tttaaagaca atatcgccaa ctattttggt
    27121 cagtggccgg tggattataa gagctgggca tggagcgaaa atgccgcggt aatggaaaag
    27181 tttaatatcc cccgccagat gctttttgac gtgcaaatgc cgggcactgt tcttgggcat
    27241 atcacaccac aagctgcgct ggcgacacat ttcccggctg ggctgccggt tgtttgtacc
    27301 accagtgata aaccggtaga agctctggga gccgggttac tggatgatga aacggcggta
    27361 atttctcttg gcacctatat cgcgttgatg atgaacggca aagcactgcc gaaagatccg
    27421 gtcgcgtact ggccgattat gtcttctatt ccgcaaacat tgctgtatga aggttacggt
    27481 attcgcaaag gcatgtggac ggtgagctgg ctgcgcgaca tgttaggcga gtcgttaatt
    27541 caggatgcca aagcacagga tctttcaccg gaagatttgc tcaacaaaaa agcgtcttgc
    27601 gtgccacctg gttgtaatgg gctgatgacg gtgctggact ggctgaccaa tccgtgggaa
    27661 ccgtacaaac gcggaattat gatcggcttt gattccagta tggattacgc atggatatat
    27721 cgttcgatac tggaaagcgt ggcgctgacg ctgaagaata attacgacaa tatgtgtcat
    27781 gaaatgaatc actttgcaaa gcatgtgatc attactggcg gcggttcgaa cagcgatctg
    27841 tttatgcaga tttttgccga cgtgttcaac cttccgacac gccgaaacgc cattaacggt
    27901 tgtgcaagtc tgggggctgc gatcaatacg gcggtaggtc tggggctata cccggattac
    27961 acaaaggctg tcgataaaat ggttcgcgtg aaagatatct tcataccgat tgagagcaat
    28021 gccaaacgct acgacgcgat gaataaaggc attttcaaag acctaaccaa acatactgat
    28081 gtgatcctga aaaaatcgta tgaagtgatg catggggaat tggggaatgc ggattcgatc
    28141 cagagctggt cgaatgcgta agtgagataa acagtgccgg gtagatgatt aatcccggca
    28201 tattcacgtt tccactacaa aataccgggt aaccactgct gaatattgtt ctctcctgag
    28261 cagcgtttat gctttaattg ctaaaccgat ttaatttgtt tagcagttag tatattggag
    28321 cctttatgag cacaagagta tgggtactcg gtgatgcggt tgttgattta ttacccgaaa
    28381 gccaggggag actactacag tgtcctggtg gggcgcctgc taatgttgca gtcggtatcg
    28441 caaggctggg gggggaaagt gcctttattg gcaaagttgg cgatgatcct ttcggtcgct
    28501 ttatgtatca gacactgagt aaagaaaatg ttgatacacg ttatatgtct cttgatcctc
    28561 aacatcgcac ctcaattgtg gctgtaggac ttgatgagca aggagaaaga aactttacct
    28621 ttatggtacg cccaagtgcc gatctttttt tacaacctga tgaccttcct acatttgggc
    28681 cgggtgaatg gctccatctt tgttccattg cgctcagtgc agaaccttcc cgaagtaccg
    28741 catttctggc tatggagaaa atacgtcagg ctggcggaaa catcagtttt gatcccaata
    28801 tccgcagcga tctctggcag agtgaagcgc tattaagaaa ataccttgat cgcgcgcttt
    28861 cgctggcgaa tatcgctaaa ttgtccgaag aagagttgct attcatcagt ggcgaaagcc
    28921 aggttcagca aggcgcatat tcattagtac aacgttattc gttgacttta ttgcttatca
    28981 cacaaggaaa aaatggcgta cttgtgtatt ttcaggggca gtttatccac tatcccgcca
    29041 aacctgtttc tgtcgtcgat acgaccgggg caggagatgc ttttgtcgct ggattacttg
    29101 caggtctggc tgattctgga ataccaacaa ataccagaca gcttgaacga atcattgcac
    29161 aagctcagat ttgtggtgct ctggcgacca cggctaaagg cgcgatgacc gccttacccc
    29221 gacaacacga tctcccttca caatagtgat cctgctgttg tatctgtaac ccgatttgtc
    29281 gtgcctgtca catttactaa atcggtttag cgaaaataat tgctctggct aaaaagtcag
    29341 ctttattatc aacacctaaa ccggtttagc attttagcaa tttttataat tctctgatgg
    29401 gacgcgacaa attatgtata aaaaaacaac tttggcagtg ttaattgctt tgctgaccgg
    29461 tgctacaacg gtacatgcgc aaacggatat tagcagtatc gaatctcgac tggcggcgtt
    29521 ggaacaacgt ttaaaaaatg cggaatcccg cgcccaggcg gcagaagcaa gggccaaaac
    29581 agctgaatta caggttcaga aactggctga aacacaacaa caaaatcagc taacaactca
    29641 agaagtagca cagagaacag ttcagctcga acagaaatcc gcagaaaaca gtggttttga
    29701 gtttcatggc tatgcccgtt ccgggttact gatgaatgat gccgcttcca gtagcaaaag
    29761 tgggccgtat ctgactcccg caggtgaaac tggtggagct gttggccgtc tgggaaatga
    29821 agccgatacc tatgtcgagt taaatgtaga acataaacaa acactggata acggtgcgac
    29881 cacacgcttt aaagcaatgt tggctgacgg acaaagagat tacaacgact ggactggcgg
    29941 ctccagtaac ctgaatatcc gacaggcttt tgccgaactg ggcgcattac caagttttac
    30001 cggagcattc aaagacagta ctgtctgggc tggtaaacgc tttgatcgcg acaattttga
    30061 tattcactgg ttagactccg atgtcgtatt tttagcggga acgggcggcg gtatctatga
    30121 cgtaaaatgg aacgatacat tccgcagtaa cttttctctc tacggacgta atttcggcga
    30181 tcttgatgat atcgacaata acgttcagaa ctacatcctc accatgaatc attatgcagg
    30241 ccccttccag ttgatggtta gcggattacg ggcaaaagat aatgatgatc gaaaagatgc
    30301 caatggtgat ctcattcaaa ctgatgctgc aaatactggc gtacatgcgt tagttggtct
    30361 gcacaatgac actttctatg gcctgcgtga agggacggca aaaacagcac tgctatatgg
    30421 ccatggcctg ggtgcggaag tcaaagggat tggctccgat ggcgctctgc tgtctgaggc
    30481 gaatacctgg cgcttcgcat cttacggcac aacacctctg ggaagcggtt ggtatgttgc
    30541 gccagcaatt ctcgcacaaa gcagtaaaga tcgttacgtc aaaggcgata gctacgaatg
    30601 ggtgaccttc aatacacgtc tgatcaaaga ggtaacacag aattttgctc tggcctttga
    30661 gggtagctat caatatatgg atctgaagcc aaaggggtat caaaaccaca acgccgtaaa
    30721 cggcagcttc tataaactca cctttgctcc aactctaaaa gctaacgata tcaataattt
    30781 ctttagccgt ccggagcttc gcctgtttgc cacctggatg gactggagca gcaaacttga
    30841 tgattttgcc agcaatgacg ctttcggcag cagtggtttc aatactggcg gagagtggaa
    30901 ttttggtgtc caaatggaaa cctggtttta ataccagaca gcctcacgga tgtgaggctt
    30961 ttactgaaat aacaatgaat tagagggtta tatggatttc gataaaatcg cccaatcgtt
    31021 gcttccttta ttaggtggta aggagaacat cgccagtgca gcacattgcg ccactcgcct
    31081 acggctggta ctggttgacg acacacttgc cgatcaacat gccattggcc agattgatgg
    31141 agtgaaaggt tgttttcgta attcagggca aatgcaaatc atctttggca ccggcgtggt
    31201 taataaagtc tatgctgcct ttattcaggt tgcaggtatt agcgaatcca gtaaagcaga
    31261 taccgctcga ctcgccgctc aaaaactcaa tccttttcag cgaatagcac ggctactttc
    31321 taatattttt gtccccatca ttccagccat tgttgcttct ggtttattaa tggggctcct
    31381 gggaatggtg aaaacatatg gctgggtgaa tgccgataat gcgatttata tcctgctgga
    31441 tatgtgcagt tcagccgcat ttattattct ccccatcctt attggcttta ctgctgctcg
    31501 tgaatttggt ggaaatcctt atcttggcgc gacattagga gggattctga ctcacccggc
    31561 actcacaaat gcctggggcg ttgctgcggg atttcagaca atgaacttct tcggttttga
    31621 aatcgccatg attggctatc agggaacggt tttccccgtg cttctggcag tatggtttat
    31681 gagcatagtg gaaaaacagt tacgcaggtt tatccctgat gctctggatc tcattctgac
    31741 gccatttctg actgtcgtca tttctggctt tatcgctctt ttgattattg gtccggcagg
    31801 acgagcgtta ggtgacggta tctctttcgt tctcagcacg ctgattgcac acgcaggctg
    31861 gttagcaggg ttgttattcg gcgggctata ttcagcgatt gttattacgg gtattcatca
    31921 tagcttccac gcaattgaag caggactttt aggtaaccct gcaatcggtg ttaatttcct
    31981 gctgcctatt tgggcaatgg caaacgttgc gcaaggcggt gcatgtctgg cggtatggtt
    32041 taaaaccaaa gatacaaaaa ttaaagcaat caccctaccc tcggcctttt ccgcaatgtt
    32101 agggattact gaagccgcta tttttggtat caaccttcgt tttgttaaac cttttattgc
    32161 ggccttaatt ggtggcgctg ccggcggtgc ctgggttgtc tctgttcacg tttatatgac
    32221 tgccgttggt ctgaccgcaa tacccggcat ggcaatcgtt cagccaacat cgttggttaa
    32281 ttatattatt ggaatggtga ttgctttcgc tgtcgctttt agcctctctt tattactcaa
    32341 atacaaaacg gacgaggagt aatgtcgatg ccttcacaaa tgcccgcgat tttacaggca
    32401 gtgatgaaag gtcagtccaa agcactggca gatgcccact atccttgttg gcatctcgct
    32461 ccagtcaccg gactaatgaa cgatccaaat ggcttttgtt ggtctggagg ccgctatcat
    32521 ctgttttatc aatggaatcc actggcttgt gatcataagt acaaatgttg ggggcactgg
    32581 agttcgacgg atttattgca ctggcagcat gaaccattag cgctaatgcc cgataaagag
    32641 tacgaccgta atggttgtta ctcaggaagc gcggttaata atcagggcgt tctcacactt
    32701 tgctataccg gtaatgttaa gtttgatgat ggcagcagaa cggcatggca atgcctggcc
    32761 accgaaaata accagggcgg gtttgataaa ctaggccctg tcataccgtt acctgatggt
    32821 tacaccggac atgttcgtga tccaaaagtc tggaagcaca acagccaatg gtacatggtg
    32881 cttggcgcgc aagataaaga gaaacggggg aaagtgctgc tgtattcctc tgtcgatctc
    32941 tatacctggt cttttcatgg agaaattgct ggtaacggtc tgaatgaaat agataacgca
    33001 ggatacatgt gggaatgtcc tgattttttc gcccttgatg gtgaatacat tctcctctgt
    33061 tgcccccagg gaatggtgcg cgaacatgag cgctatctga atacttatcc ctgcgcctgg
    33121 ctacatggac agtttgatta cgagacaggc aaatttacgc atggcgcttt ctcagagctg
    33181 gatgcgggat ttgaattcta tgcaccacag acgatggaag ccccggacgg gcgacgattg
    33241 ctggtcggct ggatgggggt tcctgatggc gaagagatgt tgcagccaac cagaaagcat
    33301 cattggcagc atcaaatgac ctgttttcgc gaactctcat tccagcaggg aaaattgttt
    33361 cagatgccaa tcagggaact cgcgcaactg cgtgaagctg aacacttctg gcaaggaaaa
    33421 gcggatcatg cgccgcatgt cgaaattgaa cgcctggaaa tggacatcat tccgtcgggt
    33481 gagctgtatc tgaattttgg caatgcgctg gcactgcatc ttaatgacga tggtattcaa
    33541 ctacagagga gaagccttgc agggcaagag aaattaactc gttactggcg aggaagcgta
    33601 acgtcactga agattctctg tgacagttcc agcgtagaga tattcatcaa taatggtgaa
    33661 ggtgtaatga gtaatcgtta ttttccccat catccagctt cgctgatatt acagggtgag
    33721 tctgacgtca cgttacgcta ctggtcgcta cgcgcctgca tggtagaatg agggttttga
    33781 attacggatc ctcgtcgtga gaaaaaccaa acgcgttact attaaagaca ttgctgaact
    33841 ggcgggagtg tcgaaagcaa ccgccagtct ggttctcaat ggtcgtggca aagagttacg
    33901 tgttgcgcag gaaacgcgtg agcgtgtgct gagtattgcc caacagcagc attatcagcc
    33961 cagcattcat gcccgctcat tacgggacaa ccgcagtcat accattggtc tcgtcgtccc
    34021 tgaaatcact aactacggat ttgcagtatt ttcccatgaa ctggaaacat tgtgtcggga
    34081 agcgggcgtc cagctcctca tatcctgtac tgatgaaaat ccttcacagg aaacaatggt
    34141 ggtgaacaat atgattgccc gccaggttga tgggttaatc gtcgcatcca gtatgctgca
    34201 tgataatgac tatcaaaaac ttagcgagca attacctgtt gtgttgttcg atcgccacat
    34261 gaacggcagt acattgccat tggtcattac tgactctgtt tctcccaccg ccgctctggt
    34321 ggcagatatt gcacggagtc acccggatga attctatttt ctgggcggac aaccccgttt
    34381 gtcaccgact cgcgatcgac tggaaggatt tacacaaggc ttgcagcaag ccggagtaac
    34441 gctacaacct gaatggatta ttcacggtaa ttatcatccg agctcaggct atgaaatgtt
    34501 tgccgcgcta tgcgctcgtt taggacgtcc ccctaaagcc ttattcactg ccgcttgtgg
    34561 attactggaa ggcgttctgc gctatatgag ccaatataat ttactcgaca gtaaaataca
    34621 cctggcgagt tttgatgatc attacttgta tgattcactt tcggtaagaa tcgacacaat
    34681 acaacaggat aatcgccaac ttgcttttca ctgctttgaa cttatttcgc aactgattga
    34741 aggtgaaacg ccatccccat tacagcgtta tcttcctgcc agtctacaaa aacgctaccg
    34801 ataatcccgt cactatcttg catcgcccct gtaaaacggg gcgattattt tcatcctgaa
    34861 cgttaaatat taatgatgaa taatcaaatc aggcaatatt caaatattta tgtgtctgca
    34921 tcgacaaacg ccagttgcgc gcaatgcagg tatcgataca taaacgcgta gcatcttcct
    34981 tctggctaat tggctgaagt gcaatgactc gcggtttatc atcggtcagc gtcgccagca
    35041 attcgtccag tgcttcaata tcacgtacgc gtcccactgg atgcttgatt tcgttggctc
    35101 gctccagggc ttgcgacaat acttcatagc cgccgcgcat gttcagcttt ggcgatacgg
    35161 taacccaggt attcggtgtg cagcgtacct catgagtacc actggtttcg atctggcagc
    35221 taaaaccgtt cttttcgagc aggtcagtca gtggcagcaa atcatgaatg caaggctcac
    35281 cacccgtaat caccacgtga cgcgcggtgt atccctgacg gccaatgaca gccagcaaat
    35341 cttcactgct cgccgccccc cacttatcac tctctttggt cttcgccaga atgctgaaaa
    35401 gggagacttc ccgatcctca agcttttccc aggtgtgttt ggtgtcgcac caggcacagc
    35461 caaccggaca tccctgtaaa cgaataaaaa tggcggggac gccggtaaag taaccctcac
    35521 cttgcagggt ctggaacatc tcgttaatcg ggtactgcat agcattctct gtgaagtgga
    35581 taattgttaa ttattgcaga tctcgccgca atattcatgc tttattaaca ttctgttaca
    35641 ggcaggttta aagaaaaaag cgaaacatat cgttaataat aaaagggagt gacgtattat
    35701 gtcagaaaga aataaagtaa agggatttta tcatgtcaaa aacattcacc aaaattatat
    35761 ttatcttttc tgtattcatt tttaattata acgaatctca aattaccgat accgccgtca
    35821 atttatttat ccagctttaa taggacattt ataatgcgct atttcatact aatattcaca
    35881 ctcgtgtgtt cctttgttgc agcccaacca actattgtcc cacaattaca gcaacaagtt
    35941 acggatctta cgagtagctt aaactcacaa gaaaagaaag aactgacgcg caagttagaa
    36001 tctattttca ataataccca agtacaaatt gctgtattaa ttattcccac aaccaaagac
    36061 gagacaattg aacaatatgc tgcaagggtt tttgacaatt ggcgtttagg agatgccaaa
    36121 cgcaatgatg ggatactgat cattgttgcc tggtcggatc gcactgtccg catccaggta
    36181 ggttatgggc tggaagaaaa ggtaaccgac gctctggctg gagatatcat ccgtagcaac
    36241 atgatacccg catttaaaca acaaaaatta gctaagggat tagagttagc tataatcgct
    36301 ttgaataacc gactcacttc acaacaccaa tattcggtta atccttcaga aagtgaatca
    36361 gcatcttcca gcgatcatta ctactttgct attttttggg tatttgcagt gatgttcttc
    36421 cctttctggt tttttcatca agacagtaat ttttgtcgcg tatgtaaaag cagtgtctgt
    36481 atttcagcaa tctatctttt agatttattc ctgttctccg ataaaacttt ttccattgct
    36541 gtatttttct tcttttttac tttcaccacc attatggtct ttacctgttt atgtgtactt
    36601 cagaaaaaag catctggtag aagttatcat tcagacaaca gcggttccgg tggtggctca
    36661 agtggcggcg gatttagcgg cgggggcggt tcttctggcg gtggcggcgc gtccggtcgc
    36721 tggtaactca accataaaaa atgccagccc gaaggctggc atttttaaat cagataaagt
    36781 cagacttatg cctggccttt gatctcttta cgaccgttgt acggtgcttt ttcgcccaga
    36841 gcttcttcga tacgaatcag ctggttgtat ttagcaacac ggtcagaacg gctcatagaa
    36901 ccagttttga tctggcctgc agcagtacca acagccaggt cagcgatggt agcgtcttca
    36961 gtttcgccag aacggtgaga gataactgca gtgtagccag cgtctttcgc catcttgatt
    37021 gcagccagag tttcagtcag agaaccgatc tggttgaatt tgatcaggat ggagttagcg
    37081 ataccttttt cgataccttc tttcaggatc ttggtgttgg ttacgaacag gtcgtcacca
    37141 accagctgga ttttgtcgcc cagaactttg gtctggtatg cgaaaccgtc ccagtcagat
    37201 tcgtccagac cgtcttcgat ggaaacgatc ggatactgtt tggtcagttc ttccaggaag
    37261 tgagtgaatt cttcagaggt gaacgctttg ttgccttcgc cagccagaac gtatttaccg
    37321 tctttgtaga attcagaagc tgcgcagtcc atcgccagag tgatgtcttt gcccagttca
    37381 taaccagcag ctttaacagc ttcagcgata acagccagag cttcagcgtt ggaacccagg
    37441 ttcggcgcat agccaccttc gtcaccaaca gcagtgttca tgcctttcgc tttcagaact
    37501 tttgccaggt gatggaaaac ttcagaaccc atacggatgg cttctttcac agttttcgcg
    37561 ccaaccggct gaatcatgaa ttcctggata tcaacgttgt tgtcagcgtg ctcaccaccg
    37621 ttgatgatgt tcatcatcgg aaccggcata gagtatttgc ccggagtgcc gttcagttca
    37681 gcgatgtgct cgtacagcgg cataccttta gcagctgcag cagctttggc gttagccaga
    37741 gatacagcca ggattgcgtt cgcgccgaat ttggatttgt tttcggtgcc gtccaggtcg
    37801 atcatgatct tgtcaatgcc agcctgatct ttagcatctt tgccaatcag cgcctgagcg
    37861 atcgggccgt ttaccgcagc aacagctttg gttacgcctt tacccaggaa acgggatttg
    37921 tcgccatcgc gcagttccag agcttcacgg gaaccagtag aagcacctga cggagcagct
    37981 gccataccga cgaaaccacc ctccagatgt acttcggctt caacagtcgg gttaccacgg
    38041 gagtcgatga tttcacgacc gatgattttt acgattttgg acattaggtt ttcctcaagt
    38101 cactagttaa actgaaactc cagacaaaca acgcgtaccc agggtacgcg ttgccgttct
    38161 aactttttta cttacttcgc ctgacgtttc tggaactcgc tggcggcttt cacaaagcct
    38221 gcaaacagcg ggtgaccatc acgtggagta gaagtaaact ccggatggaa ctggcaagcc
    38281 acgaaccacg gatgattcgg aacttcgatg atctcgacca actgatcatc cccggaacgg
    38341 cccgcaacgc gcagacctgc atcttcaatc tgtttcaaca gcatgttgtt gacttcgtaa
    38401 cggtgacgat gacgctcaac aattgtcggc gcattgtaca gctggcgaac caggctatcg
    38461 tcaaccaact ggcactgctg tgcgccgaga cgcatggtac cgccgagatc gctcttctcg
    38521 ctacgaactt caacgttgcc gttttcatcg cgccactcgg taatcagcgc cacaaccggg
    38581 tacttacagt ctggcacaaa ttccgtagag ttggcgttct ccatgttggc aacatggcga
    38641 gcgtaatcaa ttaacgccac ctgcataccc aggcaaatgc ccagataagg aatattgttc
    38701 tcacgcgcaa aacgcgcggt cgtaatcatg ccttctacgc cacgatagcc gaaaccgcca
    38761 ggtacgagga ttgcgtccag acctttaagg atttcaacgc cgcgcgtttc aacatcttgt
    38821 gaatcgatca gtttgatgtt gacgctgaca cgattcttca gcccaccgtg tttcagtgct
    38881 tcgatcacgg atttgtatgc atccggcagc tcaatgtatt tgccgaccat accaatggtg
    38941 acttcactta ccggattcgc ttcttcgaag ataacctgtt cccattcgga cagattcgct
    39001 tccgggcagt ctaagctgaa tcgtttacaa atataatcgt ccagcccctg agatttcaac
    39061 aggcccggaa ttttatagat ggaatcgacg tctttcagag aaataaccgc tttttccgga
    39121 acattacaga acaatgcaat cttcgcacgt tcgttcgccg gaacggcgcg atctgaacga
    39181 caaatcagga tatcaggctg gataccgatt gaaagcagct cttttacaga gtgctgagtc
    39241 ggtttggttt tgacttcacc agacgctgcc atgtacggca ccagcgtcag gtgcataaac
    39301 agggtgtgct cacggccaat ttcaacagcc atctggcgaa tcgcttcgag gaacggcagg
    39361 gattcgatat cacctacagt accgccgatt tcgaccagta ctacgtcatg accttcgccg
    39421 ccttccagca cgcgctcttt gatagcgtta gtgatgtgcg gaataacctg tacggttgcg
    39481 ccgagatagt caccgcggcg ttctttacgc agaacgtcag agtagatacg ccccgtggtg
    39541 aagttgttgc ggcggctcat tttggtacga atgaaacgct cgtagtgccc caggtccagg
    39601 tcggtttcag cgccgtcttc agtaacgaac acttccccgt gttggattgg gctcatagta
    39661 cctggatcga cgttgatgta cggatccagt ttcatgatgg tcacattgag gccacgggct
    39721 tcaagaatgg ctgcgaggga ggctgcggca atgcctttac ccagagagga tacgaccccg
    39781 ccggtcacaa aaatatagtt cgttgtcatg ctgaacctga gaagttaggt tgaaaagacg
    39841 atggaataac caggacggga aagcagtata cccgaacatg acctgtgcca caaactttca
    39901 ttatccctcc tcttcgccag cgcactattg aaatcaggag tgagaaaata gcccctttgg
    39961 ggtaaatgtt tttgacgcaa atcaagcgct tgtcatttaa aaaatgacac aaatcgcgct
    40021 tgaccgcgta attcccttag agatcaattt cctgccgttt tacctgttgc cagacttctt
    40081 ccattgtttc gaggtcaaca cctgtcattt ccagtccacg cgcggcaaca atacgctcca
    40141 cttcgcgaaa acgacgctcg aatttttcgt tcgctttttg caatgcgatt tccgcttttg
    40201 tccctaaatg acgagccaga ttgaccgtgg caaacagcag gtcacccatt tcctcctcca
    40261 gtttagcctg gtcgacaaca gcctgccgcg cctcgtacat cacctcgtcg atctcttcgt
    40321 agactttatc gactaccgga ccaagcgtcg tccagtcgaa accaacgttg gcgcaacgtt
    40381 tctggatttt ttgcgcacgc attaaagccg gtaaactacg aggaatatcg tccagcgccg
    40441 aatgctgcgc tttctgcgcg cgctcttcgg ttttgatttg ctcccaacgg gcaagcactt
    40501 cactactatt ttcggcagaa ctgtcagcaa aaacatgcgg atggcgacgc tctaatttat
    40561 cgctaatagc agcgcaaata tcattaaagt caaagcgccc ttcttcctga gccatttgtg
    40621 cgtaaaacac cacctggaac agcagatcgc ccagttcacc gcgcagatcg tcaaaatctt
    40681 cacgggcgat ggcgtccagc acctcgtagg tttcttcaag ggtgtaaggc gcaatggtgg
    40741 caaatgtctg ctctttatcc cacgggcagc cgttttccgg gtcgcgcagg cgctgcatga
    40801 tggtgagcaa acggtcgatt tgattcattg aattatcctg aaaattgcgg gtctgtcagg
    40861 tggaaacctg tgaccagaat agaagtgagt tagtaacacc tacccaatca gcacgttaat
    40921 tttggcttta atgagttgta attcctctgg ggcaactgtt cctttcttcg ttgctcctct
    40981 tgcccgccag gcgatacttt ttacctgatc agctaacgct acgccatcac gttcctgacc
    41041 ggatagaaca acttcgaacg gatatccttt tgattgcgtt gtacaaggaa cacacagaca
    41101 catacctgtt ttgttgttgt acatgaacgg actcaggaca acagccggac gatgtccggc
    41161 ttgctcgcta ccttttgtcg ggtcaaaatc aacccaaatc agatcgcccg tatcgggtac
    41221 gtatcggctt accattacca gacttcctta tctttcggct ccccccagtc gatattctcg
    41281 tggaggtttt ccggcgtgat gtcgttgacc agttcagcaa gcgtaaatac gggctcttta
    41341 cgcactggct caataattaa tttgccatcc accagatcaa tcttcacttc atcatcaata
    41401 ttcagattga gcgcctgcat taacgtagcc gggatccgca ccgccggtga atttccccaa
    41461 cgctttacgc tactgtggat cataaccctt tcctcaaacc gcgatcatat gtagatacag
    41521 tatatatcaa tctacattgt agatacgaac aggtttcggc ctaactcccg tgcaaccgac
    41581 gcgcgtcgat aacatccggt acctggttga gtttacccag cacgcgaccc agcacttgca
    41641 ggttgtaaat ctcaatggtc atgtcgatgg tcgccagttg ctgtttggtg tcgctacggc
    41701 tggcaacgcc aagtacgttc accttctcgt tggcgagaat ggtcgtgata tcacgtaaca
    41761 acccactacg atcattagcc accacgcgga ccaccagcga atatccggcg gagtagctct
    41821 cgccccatac cgcgtcaaca atgcgttctg gcgcatggga gcgcagttcc gccaattgtt
    41881 cgcaatcggc gcggtgtact gaaataccgc gcccctgggt aatgaagccg acaatctcat
    41941 ctccaggaat cggctggcag cagcgcgcga tgtggtgcat caggttacca acaccttcaa
    42001 ccaccacacg accgttatct ttactgcggt tttgcggcgt gtagcttttt tgctgaagct
    42061 gtttcagcgc ggcggcgtcc tgctcttcgg cactcggctt attaaattgc gattgcagga
    42121 agttcaccat ctgattgaga cggatatccc cgccaccaat cgccgccagc aactcgtcga
    42181 catcattgaa gttgtaacgc ggcagcagat gtttttctgc ttctttcagg ctgatcccca
    42241 gatgttccag ctcgtcgtca aggatttgcc gcccagccag aatgtttttg tcacggtcct
    42301 gtttacggaa ccaggcgtga attttcgaac gcccacggct ggttgtgacg taaccgaggt
    42361 ttgggtttaa ccagtcacgg ctggggttcg gctgtttctg ggtgataatt tcaatctggt
    42421 cgcccatctg cagctggtag gtgaacggca caatgcgccc gccaattttt gccccgatgc
    42481 agcggtgtcc gacatcactg tggatgtggt aagcgaagtc cagcggcgtt gatcccgcag
    42541 gcaaatcaac gacatcacct ttcggcgtaa agacgtacac tcggtcgtca aagacctgac
    42601 tacgtacttc gtcgagcatt tcgccggaat cagccatctc ttcctgccac gcaatcagtt
    42661 tacgcagcca ggcaatccgg tcttcatgtc ccgaacgtgc gccgccagca gccgcgccct
    42721 ctttatattt ccagtgcgca gcaacaccca actctgcatc ttcatgcatc tgtttggtgc
    42781 ggatttggat ctcaacggtt ttgccacccg gccccagaac cacggtatga atggactgat
    42841 aaccgtttgg tttcgggtta gcgacgtaat cgtcaaactc atccggcagg tggcgatagt
    42901 gagtgtgcac tatccccagt gcggcataac aatcctgtaa acgctcggcg acaatacgta
    42961 ccgcacgcac atcaaacagc tcatcgaagg cgaggttctt tttctgcatt ttgcgccaga
    43021 tgctgtagat gtgtttcggt cgaccataca cttcagcttt aacaccttca gctttcatct
    43081 cagcgcgcag atggccgaca aactcttcga tatagtgttc gcggtcgaga cggcgttcat
    43141 gcagcagttt tgcgatgcgt ttgtattcgg tcgggtggag ataacggaag cagtaatctt
    43201 ccagctccca tttcagttgc ccaatcccaa gacggttcgc caacggcgcg tagatattgg
    43261 tgcactcttt tgccgccagt acacgttcat cttccggcgc atcttttact tcgcgcagat
    43321 gagcaatacg ctccgccagt ttgatgacta cgcagcgaaa atcatcgacc atcgccaata
    43381 acatccggcg aacgttatcg acctgttcgg aggaaacaga atcagtgtgc gtcgctttca
    43441 gctggcggat cgccgccata tcacgcacgc cgtgaataag gttaacgacc gacttaccga
    43501 cgctctcacg cagcacatct tcgctgacta cgttggcatc agccagaggg aacagcagcg
    43561 ccgcccgcag cgtgtcaatg tccatactta atgtcgagag gatctccacc atctcaacac
    43621 cacgccacaa taacagactg gcatccggat gcccctgcgt ctgttgcaga caatacgccc
    43681 aggtttcggc taagcactca cacgacttct ggctggtaat acccagactt gcgatccatt
    43741 tttccggatc aaattcacca gccttattga tatgtgcact tcttaccgca accatcgtcc
    43801 tctcctttag ggaccagacc tgccgaaatc ggcaaatcgt aactatttaa cgcgcgagaa
    43861 aagtaccatc gattccagat gtcccgtgtg tgggaacata tccagcatcg ccagtcgcgc
    43921 aatggtatat cctgctttta ataacgcttc gctatcccga gccagcgttg cagggttaca
    43981 ggatacataa actatacgaa taggttccag ttttataatt tgctgcataa cacctgcggc
    44041 acctgctcgc gccgggtcca acaacacttt atcgaagccg tttttcgccc acggctgctt
    44101 tgtgacatct tcttcaagat tttcgtgata aaacgtcaca ttctgtaagc cattaagacg
    44161 cgcattctgc tggccttttt ccaccagcgc cggaacacct tctacaccca ccacactggc
    44221 agcttgtgtc gccaatggca gtgtaaagtt gcccataccg cagaacagat ccagtacgcg
    44281 atcttcaggt tccacatcca gccattccaa cgcacgcgct accattttct ggttcacacc
    44341 cgcattgacc tgaataaaat cgcgcgggct aaaagttaag cgcaacccat ttgagtcata
    44401 ccagggcatc tcaccagaga cggtttcgag tatctcacta tcgggggcga gatacagatc
    44461 caggccttca gaatgcgaaa agcattccag tttttcgcga tctgccgaac ttagcggtgc
    44521 ggtatggcgc aaaatcatta gcgtgccgct ggttgcctgt accagttcaa catgaccaag
    44581 atggcgcata gcttgtaagc tgcccaggca tgccctgact ttgggcagca atgcttcaag
    44641 ttggggcact aaaatggggc attgtttcac gtcgacaatg tcactggagc ccgctttgcg
    44701 aaaccccatc tgaagttgct gtgttttcgg taagtagttc agacttaaac gcgcgcgacg
    44761 gcgatagccc cagggaacat cggcgatcac ttcagagact tcgtgtttca ttaatcgggc
    44821 gagtgccgca cttttgcttc gctgctgtaa atccacactg gcgtgttgtt gctgacagcc
    44881 gccgcatacg ccaaaatgag gacagcgtgg cgtttcgcgt tccgggctat cgcttaaccg
    44941 acgtacgact ttagcgcggg catactgttt tttatcttca gtaacagtaa cttccgcgtt
    45001 ttcctgcggc aataatccgg ggataaacaa cgttttgccg ttatgtcgcg ccacaccctg
    45061 accaaaagag tcgaggtcgt tgactgaaac ggttatgatc tgacgcgtcg tcgtacgtcg
    45121 ttttgcagag tagaattgcg ccattggcga gactttctca atttaacagt gtgaccttaa
    45181 ttgtcccata acggaactcc atgaccaact acagcctgcg cgcacgcatg atgattctga
    45241 tcctggcacc gaccgtcctt attggtttat tgctgagtat ctttttcgtc gtgcatcgct
    45301 ataacgactt gcagcgtcaa ctggaagatg ccggtgccag cattattgag ccgcttgcag
    45361 tttctactga atatggcatg agcctgcaaa atcgcgaatc tatcggtcag ttaataagcg
    45421 tactgcatcg tcgccattcc gatattgttc gcgcgatttc ggtttatgat gaaaataacc
    45481 gactctttgt cacctccaat tttcatcttg atccctcatc aatgcagctc ggcagcaacg
    45541 tgccgtttcc tcgccagctc actgtcactc gtgacggcga tataatgatc ctccgcacgc
    45601 cgattatttc tgaaagctac tcccccgacg aatcgcccag tagcgatgcc aaaaatagtc
    45661 aaaatatgct gggatatatt gcgctggagc tggatcttaa atcggttcgc ttgcagcaat
    45721 ataaagagat ctttatttcc agcgtgatga tgctgttttg tatcggtatt gcgcttattt
    45781 ttggctggcg cttaatgcgc gatgtaaccg gtccgattcg caacatggtg aataccgtcg
    45841 accgcatccg tcgcgggcaa ctcgatagcc gggtggaagg atttatgctc ggcgagctgg
    45901 atatgctgaa aaacggtatc aactcgatgg caatgtcgct ggcggcttat cacgaagaga
    45961 tgcaacacaa tatcgaccag gcgacgtccg atctgcgtga gacgctggag caaatggaga
    46021 ttcagaacgt tgagttggat ctggcgaaaa agcgcgccca ggaagcggcg cgtattaaat
    46081 ccgagtttct ggcaaatatg tcacacgagc tgcgtacacc actgaatggt gttattggct
    46141 ttacccgcct gacgctgaaa acagaattaa caccaacgca gcgcgatcac ctgaatacga
    46201 ttgaacgttc ggcaaataat ttgctggcaa ttattaatga tgttctcgac ttctcgaaac
    46261 tggaagccgg taagctgatt ctggaaagta ttccgttccc actacgcagc acgctggatg
    46321 aagtcgttac cctgctggca cattcttctc acgataaagg gctggaactg acgctcaata
    46381 ttaaaagcga cgtgcctgat aacgtgatcg gcgacccact acgattacag caaatcatca
    46441 ctaacctggt agggaatgca attaaattca ccgagaatgg caacatcgat attctggtag
    46501 aaaaacgtgc gctgagtaat accaaagtgc agattgaagt gcagattcgg gataccggca
    46561 ttggtattcc tgaacgcgat caatcgcgct tattccaggc cttcagacag gctgatgcca
    46621 gtatttcccg ccgtcatggt ggcaccgggc tggggctggt gattacacaa aaactggtta
    46681 atgaaatggg cggcgatatt tcgttccata gccagccgaa tcgcggttca actttctggt
    46741 tccacattaa tctcgatctg aacccgaaca ttattatcga agggccatcc acccagtgcc
    46801 tcgctggtaa gcgcctggcc tatgtcgaac caaattccgc agcagcgcaa tgcacgctgg
    46861 atattttaag tgaaacgccg ctggaagtgg tttatagccc aacgttctcc gcgctgcctc
    46921 ccgcgcatta cgacatgatg ttattaggca tagcggtgac cttccgcgag ccgctaacaa
    46981 tgcaacatga gcgattagcg aaagcagtat cgatgaccga tttcctgatg ctggcacttc
    47041 cttgccatgc acaagtcaat gctgaaaaac ttaagcaaga tggtatcggc gcgtgtctgc
    47101 tgaaaccatt aacacctacg cgcctgttgc ctgccctgac ggaattttgt catcacaaac
    47161 aaaacacgct tttgcctgta accgatgaaa gtaagctggc aatgacagtc atggcggttg
    47221 atgacaaccc cgctaacctg aaacttatcg gcgcattgct ggaagatatg gtgcaacatg
    47281 tggaactttg cgatagcggg catcaggcag ttgaacgggc gaaacagatg ccgttcgatt
    47341 taatcttaat ggatattcaa atgccggaca tggacggcat tcgggcatgt gagctcatcc
    47401 accagctccc gcatcagcag caaacgccgg ttatcgcggt aacggcgcat gcgatggccg
    47461 ggcaaaaaga gaaactgctt ggcgcgggga tgagtgatta tctggccaaa ccgatcgaag
    47521 aagagcgatt gcataatttg ctgttgcgct acaagcctgg cagcggtatt tcctctcgcg
    47581 tcgtgacgcc cgaagtcaac gaaattgtgg tcaacccgaa tgcgaccctc gactggcaac
    47641 tggcactacg ccaggcagca ggaaaaaccg atttagcgcg cgatatgctg caaatgttac
    47701 tcgatttcct gcctgaagtt cgcaacaaag ttgaggaaca gctggttgga gaaaacccgg
    47761 aaggcctggt tgatttgatt cataaactgc atggcagttg cggctatagc ggtgtaccgc
    47821 gtatgaagaa tctctgccaa ctgatcgaac aacagctacg tagcggtact aaagaagaag
    47881 atttagagcc ggagctgctg gaactgttgg acgagatgga taatgtagcg cgcgaagcca
    47941 gcaaaattct cgggtaatgg acatcttgtc gagtagcaac cggattttta tgacgctggt
    48001 ttcagattgg tgacaagtgc ctgtgtttat gccggatgcg gcgttaacgc cttatccggc
    48061 ctgcaaaacc aattaaattc aatgaattaa aaaatgatgt agccctgata cgtagcgcaa
    48121 caggcaattt tgcgtttggc atcagtctca aaccggctcc agatagagcc ggttttggtt
    48181 ttctgtctta acgcaccatg cacggacgct tgttatcgaa cgtccagccg gggatcaggt
    48241 actgcattcc catcgcatcg tcacgcgcgc caagcccgtg tttctgatac agctcgtgcg
    48301 ctttcatcac ttgatccata tcgatttcta cacccagccc cggtttttct ggcacctgaa
    48361 ccagaccgcc tttgatctct aacggttctt tggtcaggcg ctgattacct tcctgccaga
    48421 tccagtgcgt atcaatcgcg gtgattttac ccggtgcggc agcggcaaca tgggtaaaca
    48481 tcgccaggga aatatcgaag tggttgttgg agtgtgaacc ccaggtcagg ccaaattcat
    48541 gacacatttg cgccacacgt accgaacctt gcatcgtcca gaaatgcgga tccgcaagcg
    48601 ggatatcaac ggattgcaac gagagcgtat ggcccatctg ccgccagtcg gtggcgatca
    48661 tattggttgc ggtcggcagg ccagttgcgc gacggaactc tgccatcact tcacgcccgg
    48721 agaaaccttg ctccgcacca cacggatctt ctgcataagc cagcgaacct ttcaggtatg
    48781 taccgatttt aatcgcttct ttcagcgacc aggcaccgtt aggatcgagc gtaatacgcg
    48841 cctgcgggaa acgttgcgcc agtgccacaa tagattcggc ttcttcttcc ccggccagta
    48901 caccgccttt cagtttgaaa tcgttgaagc catatttttc atacgccgct tccgccaggc
    48961 gcaccaccgc atccggcgtc atcgcttctt catgacgcag gcgataccag tcgcatgagt
    49021 catccggctg gctttgatac ggcagcggtg tagctttgcg attgccgacg aagaacagat
    49081 aaccgagcat ttcgacttcg ctacgctgtt gaccatcgcc cagcagcgat gccacgttta
    49141 cccccagatg ctgccccagc agatccagca ttgccgcttc tatcccggta actacatgaa
    49201 tagtggtacg caaatcaaat gtctgcaaac cgcgcccacc ggcatcacga tcggcaaaag
    49261 tattacgcac cagcgtcaga acgtttttgt attcacccag cgttttaccc accaccagcg
    49321 gaatcgcatc ttccagcgtt ttacggattt tctcgccgcc gggaatttcc cctacgccag
    49381 tgtgaccgga attatctttg ataatcacaa tattacgcgt gaagaacggt gcatgtgcac
    49441 cactcagatt catcagcata ctgtcatgac ccgccaccgg gatgacctgc atttcagtaa
    49501 caacaggcgt cgtaaattga gaactcatat ttatgtcctt tttcagaatc aatgacggcc
    49561 gaaaacggga cgtttacggt caaaggtcca gccggggatc aggtactgca tcgggcctgc
    49621 gtcgttacgc gcaccgccag gcagacgttt ataggcctca tgtgcctttt gtacctgttc
    49681 ccagtccagt tccacgccca gaccgggcgc atcaggaacg gcaatttttc cgtttttaat
    49741 ctccagcgga tttttggtca gacgacaatc gccctcctgc caaatccagt gggtatcgat
    49801 agcggtagga ttacccggtg ccgccgcgcc cacatgggta aacatcgcca gagagatatc
    49861 aaagtggtta ttagaatggc agccccaggt cagcccccag tcgtcgcaaa gttgcgccac
    49921 gcggactgca ccggaaagcg tccagaagtg cggatcggca agtggaatat ctaccgcatt
    49981 gagcatcacc gcatgaccca tttcgcgcca gttggtggcg atcatgttgg tcgcgacggg
    50041 caagccggtc gcccgtcgaa actccgccat cacttcacga ccggagaaac cctgctcagc
    50101 gccgcacggg tcttccgcat aggtaagaac atcatttagc cctttgcaca gagaaatggc
    50161 ttcatcaagc agccaggcac cattgggatc aacggtaatc cgcgcatcag ggaagcgttt
    50221 cttcaatgta cgaacagtgt cgatttcttg ctcgccaggt aacacgccac ccttaagttt
    50281 gaaatcttta aagccatagc gatcctgtga ggcttccgcc agacgcacaa cggcttcgct
    50341 gttcatcgct ttctgatgcc gcaactgata ccactcatgg ttgcccggcg tattttccag
    50401 ataaggaaga tcggttttgg tccgatcacc gatataaaac agataaccga gaacggtaat
    50461 agcatcacgt tgcttgcctg gccctaacag ttcgcagacc ggaacattca gcgcctgacc
    50521 taacaggtca agcaaggcgg cttccagcgc cgccacggca ttaacgcgca attcaaaagt
    50581 ccaggcacct ttgccgaagg tatcaaaatc ggctgcctga ttacctttat gcacctgctg
    50641 aaccacttta ttcaggcgcg caacttcctg gcccagcacc atcggaatag cctcgaccag
    50701 tgtttgataa atcacctctc cgcccggcgc ttcaccaatg ccggtatgcc cggcgttatc
    50761 ggtgagtacc acaatattgc gggtgaaata tgcgttatgt gcaccaccaa tattaagcaa
    50821 catgctgtca tgtccggcga ccggaatgac tttcatatca gtaataacag gactggattg
    50881 tgtcgtcatc ttattgcccc gcaacaggtt tcagctcaat acgcttgata tcgcccacca
    50941 gcaccaggta gctgagtacc gcgattaagg catgaacacc aacataaatc agcgccccat
    51001 taaaggagcc agtcgtgcca acgatataac caattgcgat tggcgtgacg atgccagaaa
    51061 tgttaccaaa catgttgaac aggccaccgg aaagaccgct gatctctttt ggcgcagtat
    51121 cagccattac agcccaaccc agcgcaccga tccccttacc gaagaaggcc agcgccataa
    51181 agccgatgat catccactca acgttgacgt agttgcagaa caccatcacc atcgacaaca
    51241 acatgcccat cacgatcggt gttttacgcg caatgttcag tgaacccgtg cgacgcatca
    51301 gccagtcgga aataatcccc cccaacacac cgccgataaa accgcaaacc gccggaacgg
    51361 aagccacaaa gcccgctttc agaatcgaca tcccgcgcgc ctgcaccaga taaaccggga
    51421 accaggtaat aaaaaagtaa gtcagggcgt tgatacagta ctgaccgata taaacgccga
    51481 tcatcatccg tgacccgagc agctgtttga tctgccccca cttcacgctg aacggcactt
    51541 taactttggt gttttgctga tccatattga tcagcgcgcc acccgcggcg atgtactcca
    51601 gctctttctg atttacgccc ggatgttgat ttggctcgtg gatgactttc aaccagataa
    51661 agctgatgac aatgcccaga ccgcccataa agaagaagac gtgtgaccag cccacttcat
    51721 gagtcaacca gcccataatc ggcgcgaaga taaccgttgc gaagtattgg gcggagttaa
    51781 aaatcgacac cgccgttccc ctttcctgcg ccggaaacca ggccgcaaca atgcggctgt
    51841 tgccagggaa agagggcgct tcagcaagcc cgaccaggaa gcgcagtgta aacagggcaa
    51901 cgataatgcc gaatccacta aagatatcga cgaagccttg cagcaaggta aacatcgacc
    51961 agataaagat cgaccagaag tagacacgtt ttgaaccaaa acgatccagc aaccagccac
    52021 cagggatctg cccgataaca tacgcccatg agaaggcaga gaacacatag cccattccca
    52081 cgggatcaag gccgatatct ttggccattt ccgaaccggc aatagaaagc gtagcgcggt
    52141 cgccgtagtt gaaggatgtg acgataaaca acatcaccac tatccagtaa cgagcattcg
    52201 tgcgtttttc cacactgctc gcagcctgac ttaaagaact cattgttgca ctcctgaaaa
    52261 ttcgcgttag ccacgctcac tctggactgc gacatcgcca ggaaatcaga ggtgacgtag
    52321 ggtgtttttg ccgtttttat aggtcgttcg ccgaatacgg cgcgtgttta tatctggcaa
    52381 tagcagtata aaaagcgcgc catagcggct caccgtgcaa caacacaaca ttaatgcgtt
    52441 caatgaggcc cgattttggc attagccctg gacggtggaa tccacttcac ggaaatgaaa
    52501 acaagaacaa gaaaggaagg gttaaaacga agaaataaag aagagtatga aatggatcgc
    52561 ttgactccag gcaaacgcca gtaaaaatcc gcgctatgaa gcagttttta ctggcatttg
    52621 cctgaaaaga ttcgattcag caccgctaaa acggcattta ccgctcgctg aacatatcag
    52681 gacaacatcg tgccccaatg ttcaacccac ggatttgatt ccgtttccgg ttccgggttc
    52741 tcactggcgt caatcaacag catttcgcca acccgctgag cgctctgttc ctgcaacaag
    52801 gcatcgaact gtttgccgcc attgcagaaa ttcacataac tactgtcgcc gagcgcaatc
    52861 acgccataac gcagattcgg ctggaagccc agactatctt tgattccctg aaagagtggc
    52921 acaatactat caggaaggtc gccctgcccg gtcgtggacg taaccaccag aacatactta
    52981 tcctgatagg gcagccagtc gcttaattca ggatcttcaa ataccgttgc tttgtggccc
    53041 tgcgcggtca gaatcgcttc cgcttcttcg gccactaaca gtgaattccc gtacatggtg
    53101 ccgacaaaaa taccaatttc cgccataccg ttttctccct ggattaggaa cttatctctc
    53161 catcctgacc cgatggcgca ctgaactcaa ccctttcatt ttcaggaagc agaccgcgcc
    53221 agccaaattg tgataatgcc tgcatccagg tgtcgtccag gcccgcgtga atagccagcg
    53281 gctcgccagt aaaaggatgc gtcaatgaca actgactggc atgcagcatt aaccgctgaa
    53341 ggccaaaatg ctcagcaccg ctgcgattct ggcgtaaatc accatgtttg ctgtcgccaa
    53401 taatcgggtg ccgcaaatgg gcaagatggc gacgaagctg gtgcttgcgt ccagttttcg
    53461 gctccagttc caccaggccg tagcgcgtgg tcgggtaacg tccagtcgct accggcattt
    53521 ctacggtcgc cagaccgcga taatgcgtca ctgctggctg cgggccttta tcttcgcggg
    53581 caaatttatc agcgattttg tccagttctt ccaccagtgg ataatccagc accgcttctt
    53641 ccatcaacca gccacgcaca atcgcatggt aacgtttctg gatttggtgc tgttcaaact
    53701 gttgtgccag cagccgtccg gcttcgctgg ataatcccat caacaacaca ccagaagttg
    53761 gtcggtctag acgatgagca gtaaaaacat gctggcctat ctggtcacgc acggtttgca
    53821 tgaccactac tttctcgtcg cgatccagcc agctgcggtg aaccagccag ccggagggtt
    53881 tatttaccgc aaccagccat tcatcctgat agagtatttc cagcattagc tcgcatcatc
    53941 cgcaaaaaga gcatccagtt tctccagctc agccagaata agcgcgcgtt gcggatgatc
    54001 agtcgccagc gccatttcat aatagggcgc aacagcaaaa gcgcccggta acggctggtt
    54061 gttatctaac aaatcgtgca ttcgcgggat cagcacccac tgcaaccact ccagtggttc
    54121 catggtgtcc ataaagaacg gttgagtact gttaaattga tgcggcaggg gttcatcgtt
    54181 tcgccagtgc tgatgttcac gcagtaacgc ttcaagcgcc tgcaactgga gacgaacgcg
    54241 gtcatgagtg gtcataaagc aacctcaata agaaaaactg ccgcgaagaa tagcaggaat
    54301 aaaaaaaggg agcactgtat tcacagtgct cccggttcgt ttcgcagcat tccggctact
    54361 tttgttgctc cctgctcatc cttgacaact tttcctctgg ccttacggcc aatcgttcat
    54421 cctgaactat tgcttcctgc tcacaccacc ccgatgtgat acttcatcct gaagtgttcc
    54481 tggccttcct gacccaccga atcatcctga ccggttctca ttctccatcc tggaggtgtc
    54541 ctttaacgcg tcctgcgtca tcctcttcgc ttcatccaga agcctttccc tgaaacacca
    54601 tcctggtgtg tccttcagaa gtgtcatcat cctgatgttc acttcgttgt ctgactccct
    54661 gtcgacgaag atagaatcgt ctttttctcg aag
//
LOCUS       DADSYM010000007        52308 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2415
            SAMN08039579-rid11723173.denovo.007, whole genome shotgun sequence.
ACCESSION   DADSYM010000007 DADSYM010000000
VERSION     DADSYM010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039579
            Sequence Read Archive: SRR6362533
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 52308)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 52308)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980162.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 03:39:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,484
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 4,347
            CDSs (with protein)               :: 4,347
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 43 of 85
            Pseudo Genes (incomplete)         :: 37 of 85
            Pseudo Genes (internal stop)      :: 19 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..52308
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039579-rid11723173.denovo.007"
                     /strain="LGE2415"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8302 N 95.9628 W"
                     /collection_date="2015-08-24"
     gene            67..1116
                     /locus_tag="J1Y83_000344"
     CDS             67..1116
                     /locus_tag="J1Y83_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeiH family protein"
                     /protein_id="HBA7628531.1"
                     /translation="MTNITLQKQHRTLWHFIPGLALSAVITGVALWGGSIPAVAGAGF
                     SALTLAILLGMVLGNTIYPHIWKSCDGGVLFAKQYLLRLGIILYGFRLTFSQIADVGI
                     SGIIIDVLTLSSTFLLACFLGQKVFGLDKHTSWLIGAGSSICGAAAVLATEPVVKAEA
                     SKVTVAVATVVIFGTVAIFLYPAIYPLMSQWFSPETFGIYIGSTVHEVAQVVAAGHAI
                     SPDAENAAVISKMLRVMMLAPFLILLAARVKQLSGANSGEKSKITIPWFAILFIVVAI
                     FNSFHLLPQSVVNMLVTLDTFLLAMAMAALGLTTHVSALKKAGAKPLLMALVLFAWLI
                     VGGGAINYVIQSVIA"
     gene            1190..2047
                     /gene="nfo"
                     /locus_tag="J1Y83_000345"
     CDS             1190..2047
                     /gene="nfo"
                     /locus_tag="J1Y83_000345"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HBA7628532.1"
                     /translation="MKYIGAHVSAAGGLANAAIRAAEIDATAFALFTKNQRQWRAAPL
                     TTQTIDEFKAACEKYHYTSAQILPHDSYLINLGHPVAEALEKSRDAFIDEMQRCEQLG
                     LSLLNFHPGSHLMQISEEDCLARIAESINIALDKTQGVTAVIENTAGQGSNLGFKFEH
                     LAAIIDGVEDKSRVGVCIDTCHAFAAGYDLRTPAECEKTFADFARIVGFKYLRGMHLN
                     DAKSTFGSRVDRHHSLGEGNIGHDAFRWIMQDDRFDGIPLILETINPDIWAEEIAWLK
                     AQQTEKAVA"
     gene            2051..3139
                     /locus_tag="J1Y83_000346"
     CDS             2051..3139
                     /locus_tag="J1Y83_000346"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HBA7628533.1"
                     /translation="MNDREKQILKILRRNPLIQQNEIADILQISRSRVAAHIMDLMRK
                     GLIKGKGYILTEQDYCVVVGAINMDIRGMADIRYPQAASHPGSVHCSAGGVGRNIAHN
                     LALLGRDVHLISAIGNDFYGETLLEETRRAGVNVSNCIRLHGHSTATYLAIANKQEET
                     ILAINDTHILQQLTPQLLNTSRDLIRHAGVVLADCNLTPEALEWVFTIADEIPMFVDT
                     VSEFKANKVKNWYSRIHTLKPTQNELEILWGQPIKDDNDRIRAVNSLHQQGVKRIFVY
                     LKDESVFCSDKDGEKFLLTAPAHTTVDSFGADDGFMAGLVYSFLEGYSFRDSARFAMA
                     CAAISRASGSLNNPTLSADNALSLVPMV"
     gene            complement(3407..4348)
                     /gene="rihB"
                     /locus_tag="J1Y83_000347"
     CDS             complement(3407..4348)
                     /gene="rihB"
                     /locus_tag="J1Y83_000347"
                     /EC_number="3.2.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosylpyrimidine nucleosidase"
                     /protein_id="HBA7628534.1"
                     /translation="MEKRKIILDCDPGHDDAIAIMMAAKHPAIDLLGITIVAGNQTLD
                     KTLINGLNVCQKLEINVPVYAGMPQPIMRQQIVADNIHGETGLDGPVFEPLTRQAENT
                     HAVKYIIDTLMASDGDITLVPVGPLSNIAVAMRMQPAILPKIREIVLMGGAYGTGNFT
                     PSAEFNIFADPEAARVVFTSGVPLVMMGLDLTNQTVCTPDVIARMERAGGPAGELFSD
                     IMNFTLKTQFENYGLAGGPVHDATCIGYLINHDGIKTQEMYVEVDVNSGPCYGRTVCD
                     ELGVLGKPANTKVGITIDTDWFWGLVEECVRGYIKTH"
     gene            4478..5176
                     /gene="yeiL"
                     /locus_tag="J1Y83_000348"
     CDS             4478..5176
                     /gene="yeiL"
                     /locus_tag="J1Y83_000348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311082.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator YeiL"
                     /protein_id="HBA7628535.1"
                     /translation="MKEIHNNDLKQQLMSESAFKDCFSTDVSADTRLFHFLARDYIVQ
                     EGQQPSWLFYLTRGRARLYVTLANGRVSLIDFFAAPCFIGEIELIDKDHEPRAVQAIE
                     ECWCLALPMKHYRPLLLNDTLFLRKLCVTLSHKNYRNIVSLTQNQSFPLVNRLAAFIL
                     LSQEGDLYHEKHTQAAEYLGVSYRHLLYVLAQFINDGLLIKSKKGYLIKNRKQLSGLA
                     LEMDPENKFSGMMQ"
     gene            complement(5243..6493)
                     /locus_tag="J1Y83_000349"
     CDS             complement(5243..6493)
                     /locus_tag="J1Y83_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HBA7628536.1"
                     /translation="MDIMRSVVGMVVLLAIAFLLSVNKKSISLRTVGAALLLQIAIGG
                     IMLYFPPGKWAVEQAALGVHKVMSYSDAGSAFIFGSLVGPKMDVLFDGAGFIFAFRVL
                     PAIIFVTALISLLYYIGVMGLLIRILGSIFQKALNISKIESFVAVTTIFLGQNEIPAI
                     VKPFIDRMNRNELFTAICSGMASIAGSMMIGYAGMGVPIDYLLAASLMAIPGGILFAR
                     ILSPATEPSQVTFENLSFSETPPKSIIEAAANGAMTGLKIAAGVATVVMAFVAIIALI
                     NGIIGGIGGWFGFANVSLESIFGYVLAPLAWIMGVDWSDANLAGSLIGQKLAINEFVA
                     YLNFSPYLQTSGTLDVKTIAIISFALCGFANFGSIGVVVGAFSAISPKRAPEIAQLGL
                     RALAAATLSNLMSATIAGFFIGLA"
     gene            complement(6587..7525)
                     /locus_tag="J1Y83_000350"
     CDS             complement(6587..7525)
                     /locus_tag="J1Y83_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine-5'-phosphate glycosidase"
                     /protein_id="HBA7628537.1"
                     /translation="MSELTLSPELLQISAEVQDALKNKKPVVALESTIISHGMPFPQN
                     AQTAIEVEETIRKQGAVPATIAIIGGVMKVGLSKEEIELLGREGHNVTKVSRRDLPFV
                     VAAGKNGATTVASTMIIAALAGIKVFATGGIGGVHRGAEHTFDISADLQELANTNVTV
                     VCAGAKSILDLGLTTEYLETFGVPLIGYQTKALPAFFCRTSPFEVSIRLDSAREIARA
                     MAVKWQTGLNGGLVVANPIPEQFAMPEETINAAIDQAVAEAEEQGVIGKESTPFLLAR
                     VAELTGGDSLKSNIQLVFNNAILASEIAKEYQRLVG"
     gene            complement(7513..8454)
                     /locus_tag="J1Y83_000351"
     CDS             complement(7513..8454)
                     /locus_tag="J1Y83_000351"
                     /EC_number="2.7.1.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine kinase"
                     /protein_id="HBA7628538.1"
                     /translation="MREKDYIVIIGSANIDVAGYSHHPLNYADSNPGKIKFTPGGVGR
                     NIAHNLALLGKNAWLLSAVGGDFYGQSLLAQTNQSGVYVDKCLIVPGENTSSYLSLLD
                     NTGEMLVAINDMSISDCISAEFLAQHQEFIRGAKVIVADCNLSEEALVWVLENSGETP
                     VFIDPVSAWKCVKIRDHLSKIHTLKPNRLEAETLSGIALSGREDVAKVAAWFHQHGLN
                     RLVLSMGGDGVYYSDINGESGWSAPIKTNVINVTGAGDAMMAGLASCWVDGMPFIDSV
                     RFAQGCSSMALACEYTNNPELSIANVTSLVENTECLN"
     gene            complement(8910..10601)
                     /gene="fruA"
                     /locus_tag="J1Y83_000352"
     CDS             complement(8910..10601)
                     /gene="fruA"
                     /locus_tag="J1Y83_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001538991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIBC"
                     /protein_id="HBA7628539.1"
                     /translation="MKTLLIIDANLGQARAYMAKTLLGAAARKAKLEIIDNPNDAEMA
                     IVLGDSIPNDSALNGKNVWLGDISRAVAHPELFLSEAKGHAKPYTAPVAATAPVAASG
                     PKRVVAVTACPTGVAHTFMAAEAIETEAKKRGWWVKVETRGSVGAGNAITPEEVAAAD
                     LVIVAADIEVDLAKFAGKPMYRTSTGLALKKTAQELDKAVAEATPYEPAGKAQTATTE
                     GKKESAGAYRHLLTGVSYMLPMVVAGGLCIALSFAFGIEAFKEPGTLAAALMQIGGGS
                     AFALMVPVLAGYIAFSIADRPGLTPGLIGGMLAVSTGSGFIGGIIAGFLAGYIAKLIS
                     TQLKLPQSMEALKPILIIPLISSLVVGLAMIYLIGKPVAGILDGLTHWLQTMGTANAV
                     LLGAILGGMMCTDMGGPVNKAAYAFGVGLLSTQTYGPMAAIMAAGMVPPLAMGLATMV
                     ARRKFDKAQQEGGKAALVLGLCFISEGAIPFAARDPMRVLPCCIVGGALTGAISMAIG
                     AKLMAPHGGLFVLLIPGAITPVLGYLVAIIAGTLVAGLAYAFLKRPETQIVEKNA"
     gene            complement(10618..11556)
                     /gene="fruK"
                     /locus_tag="J1Y83_000353"
     CDS             complement(10618..11556)
                     /gene="fruK"
                     /locus_tag="J1Y83_000353"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HBA7628540.1"
                     /translation="MSRRVATITLNPAYDLVGFCPEIERGEVNLVKTTGLHAAGKGIN
                     VAKVLKDLGIDVTVGGFLGKDNQDGFQQLFSELGIANRFQVVQGRTRINVKLTEKDGE
                     VTDFNFSGFEVTPADWERFVTDSLSWLGQFDMVCVSGSLPSGVSPEAFTDWMTRLRSQ
                     CPCIIFDSSREALVAGLKAAPWLVKPNRRELEIWAGRKLPEMKDVIEAAHALREQGIA
                     HVVISLGAEGALWVNASGEWIAKPPSVDVVSTVGAGDSMVGGLIYGLLMRESSEHTLR
                     LATAVAALAVSQSNVGITDRPQLAAMMARVDLQPFN"
     gene            complement(11556..12686)
                     /locus_tag="J1Y83_000354"
     CDS             complement(11556..12686)
                     /locus_tag="J1Y83_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused PTS fructose transporter subunit IIA/HPr
                     protein"
                     /protein_id="HBA7628541.1"
                     /translation="MFQLSVQDIHPGEKAGDKEEAIRQVAAALVQAGNVAEGYVNGML
                     AREQQTSTFLGNGIAIPHGTTDTRDQVLKTGVQVFQFPEGVTWGDGQVAYVAIGIAAS
                     SDEHLGLLRQLTHVLSDDSVAEQLKSATTAEELRALLMGEKQSEQLKLDNEMLTLDIV
                     ASDLLTLQALNAARLKEAGAVDATFVTKAINEQPLNLGQGIWLSDSAEGNLRSAIAVS
                     RAANAFDVDGETAAMLVSVAMNDDQPIAVLKRLADLLLDNKADRLLKADAATLLALLT
                     SDDAPTDDVLSAEFVVRNEHGLHARPGTMLVNTIKQFNSDITVTNLDGTGKPANGRSL
                     MKVVALGVKKGHRLRFTAQGADAEQALKAIGDAIAAGLGEGA"
     gene            13054..14235
                     /gene="setB"
                     /locus_tag="J1Y83_000355"
     CDS             13054..14235
                     /gene="setB"
                     /locus_tag="J1Y83_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar efflux transporter SetB"
                     /protein_id="HBA7628542.1"
                     /translation="MHNSPAVTSAKSFDLTSMAFLIVAFLTGIAGALQTPTLSIFLTD
                     EVHARPAMVGFFFTGSAVIGILVSQFLAGRSDKRGDRKSLIVFCCLLGVLACTLFAWN
                     RNYFVLLFVGVFLSSFGSTANPQMFALAREHADKTGREAVMFSSFLRAQVSLAWVIGP
                     PLAYALAMGFSFTVMYLSAAVAFIVCGVMVWLFLPSMQKELPLATGTVEAPRRNRRDT
                     LLLFVICTLMWGSNSLYIINMPLFIINELHLPEKLAGVMMGTAAGLEIPTMLIAGYFA
                     KRLGKRFLMRVAAVGGVCFYAGMLMAHSPVILLGLQLLNAIFIGILGGIGMLYFQDLM
                     PGQAGSATTLYTNTSRVGWIIAGSVAGIVAEIWNYHAVFWFAMVMIIATLFCLLRIKD
                     V"
     gene            complement(14232..14486)
                     /locus_tag="J1Y83_000356"
     CDS             complement(14232..14486)
                     /locus_tag="J1Y83_000356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HBA7628543.1"
                     /translation="MECRPGCGACCTAPSISSPIPGMPDGKPANTPCIQLDEQQRCKI
                     FTSPLRPKVCAGLQASAEMCGNSRQQAMTWLIDLEMLTAP"
     gene            14641..15213
                     /gene="yeiP"
                     /locus_tag="J1Y83_000357"
     CDS             14641..15213
                     /gene="yeiP"
                     /locus_tag="J1Y83_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007750416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P-like protein YeiP"
                     /protein_id="HBA7628544.1"
                     /translation="MPRANEIKKGMVLNYNGKLLLVKDIDIQSPTARGAATLYKMRFS
                     DVRTGLKVEERFKGDDIVDTVTLTRRYVDFSYVDGNEYVFMDKEDYTPYTFTKDQIEE
                     ELLFMPEGGMPDMQVLTWDGQLLALELPQTVDLEIVETAPGIKGASASARNKPATLST
                     GLVIQVPEYLSPGEKIRIHIEERRYMGRAD"
     gene            15436..16902
                     /locus_tag="J1Y83_000358"
     CDS             15436..16902
                     /locus_tag="J1Y83_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HBA7628545.1"
                     /translation="MNTIASVTLPHHVHAPRYDRQQLQSRIVHFGFGAFHRAHQALLT
                     DRVLNAQGGDWGICEISLFSGDQLMSQLRAQNHLYTVLEKGADGNQAIIVGAVHECLN
                     AKLDSLAVIIEKFCEPQVAIVSLTITEKGYCIDPATGALDTSNPRIIHDLQNPEEPHS
                     APGILVEALKRRRERGLTPFTVLSCDNIPNNGHVVKNAVLGMAEKRSPELAGWIKEHV
                     SFPGTMVDRIVPAATNESLAEISQHLGVNDPCAISCEPFIQWVVEDNFVAGRPAWEVA
                     GVQMVNDVLPWEEMKLRMLNGSHSFLAYLGYLSGFAHISDCMQDRAFRHAARTLMLDE
                     QAPTLRIKDVDLTQYADKLIARFANPALKHKTWQIAMDGSQKLPQRMLAGIRIHLGRE
                     TDWSLLALGVAGWMRYVSGVDDAGNAIDVRDPLSDKIRELVAVSSSEQRVTALLSLRE
                     IFGDDLPDNPHFVQAIEQAWQQIAQFGAHQALLNTLKI"
     gene            17020..18006
                     /locus_tag="J1Y83_000359"
     CDS             17020..18006
                     /locus_tag="J1Y83_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HBA7628546.1"
                     /translation="MTRTNLITGFLGSGKTTSILHLLAHKDPNEKWAVLVNEFGEVGI
                     DGALLADSGALLKEIPGGCMCCVNGLPMQVGLNTLLRQGKPDRLLIEPTGLGHPKQIL
                     DLLTAPVYEPWIDLRATLCILDPRLLLDEKSASNENFRDQLAAADIIVANKSDRATPE
                     SEQALERWWQQNGGDRQLIHSEHGKIDGHLLDLPRRNLAELPASAAHSHQHSVKKGLA
                     ALSLPEHQRWRRSLNSGQGHQACGWIFDADTVFDTIGILEWARLAPVERVKGVLRIPE
                     GLVRINRQGDDLHIETQNVAPPDSRIELISSSEADWNALQSALLKLRLATTA"
     gene            18045..18758
                     /gene="lpxT"
                     /locus_tag="J1Y83_000360"
     CDS             18045..18758
                     /gene="lpxT"
                     /locus_tag="J1Y83_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Kdo(2)-lipid A phosphotransferase"
                     /protein_id="HBA7628547.1"
                     /translation="MIKNLPQIVLLNIVGLALFLSWYIPINHGFWLPIDADIFYFFNQ
                     KLVESKAFLWLVALTNNRAFDGCSLLAMGMLMLSFWLKENAPGRRRIVIIGLVMLLTA
                     VVLNQLGQALIPVKRASPTLTFTNINRVSELLSVPTKDASRDSFPGDHGMMLLIFSAF
                     MWRYFGKVAGLIALIIFVVFAFPRVMIGAHWFTDIIVGSMTVILIGLPWVLLTPLSDR
                     LITFFDKSLPGKNKHFQNK"
     gene            19170..19736
                     /gene="mepS"
                     /locus_tag="J1Y83_000361"
     CDS             19170..19736
                     /gene="mepS"
                     /locus_tag="J1Y83_000361"
                     /EC_number="3.4.17.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional murein DD-endopeptidase/murein
                     LD-carboxypeptidase"
                     /protein_id="HBA7628548.1"
                     /translation="MVKSQPILRYILRGIPAIAVAVLLSACSANNTAKNMHPETRAVG
                     SETSSLQASQDEFENLVRNVDVKSRIMDQYADWKGVRYRLGGSTKKGIDCSGFVQRTF
                     REQFGLELPRSTYEQQEMGKSVSRSNLRTGDLVLFRAGSTGRHVGIYIGNNQFVHAST
                     SSGVIISSMNEPYWKKRYNEARRVLSRS"
     gene            19917..21473
                     /locus_tag="J1Y83_000362"
     CDS             19917..21473
                     /locus_tag="J1Y83_000362"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-GMP phosphodiesterase"
                     /protein_id="HBA7628549.1"
                     /translation="MFIRAPNSGRKLLLTCIVAGVMIAILVSCLQFLVAWHKHEAKYD
                     TLITDVQKYLDTYFADLKSTTDRLQPLTLDTCQQANPELTARAAFSMNVRTFVLVKDK
                     KTFCSSATGEMDIPLNELIPALDINKNVDMAILPGTPMVPNKPAIVIWYRNPLLKNSG
                     VFAALNLNLTPSLFYSSRQEDYDGLALIIGNTALSTFSSRLMNVNELTDMPVRETKIA
                     GIPLTVRLYADDWTWNDVWYAFLLGGMSGTFVGLLCYYLMSVRMRPGREIMTAIKREQ
                     FYVVYQPVVDTQALRVTGLEVLLRWRHPVAGEIPPDAFINFAEAQKMIVPLTQHLFEL
                     IARDAAELEKVLPVGVKFGINIAPAHLHSESFKADIQKLLTSLPAHHFQIVLEITERD
                     MLKEREATQLFAWLHSVGVEIAIDDFGTGHSALIYLERFTLDYLKIDRGFINAIGTET
                     ITSPVLDAVLTLAKRLNMLTVAEGVETPEQARWLSERGVNFMQGYWISRPLPLDDFVR
                     WLKKPYTPQW"
     gene            21555..23369
                     /locus_tag="J1Y83_000363"
     CDS             21555..23369
                     /locus_tag="J1Y83_000363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311096.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBA7628550.1"
                     /translation="MIVRILLLFIALFTFGAQAQAIKESYAFAVLGEPRYAFNFNHFD
                     YVNPAAPKGGQITLSALGTFDNFNRYALRGNPGARTEQLYDTLFTTSDDEPGSYYPLI
                     AESARYADDYSWVEVAINPRARFHDGSPITARDVEFTFQKFMTEGVPQFRLVYKGTTV
                     KAIAPLTVRIELAKPGKEDMLSLFSLPVFPEKYWKDHKLSDPLATPPLASGPYRITSW
                     KMGQNIVYSRVKDYWAANLPVNRGRWNFDTIRYDYYLDDNVAFEAFKAGAFDLRMEND
                     AKNWATRYTGKNFDKKYIIKDEQKNESAQDTRWLAFNIQRPVFSDRRVREAITLAFDF
                     EWMNKALFYNAWSRTNSYFQNTEYAARNYPDAAELVLLAPMKKDLPPEVFTQIYQPPV
                     SKGDGYDRDNLLKADKLLNEAGWVLKGQQRVNVTTGQPLSFELLLPASSNSQWVLPFQ
                     HSLQRLGINMDIRKVDNSQITNRMRSRDYDMMPRVWRAMPWPSSDLQISWSSEYINST
                     YNAPGVQSPVIDSLINQIIAAQGNKEKLLPLGRALDRVLTWNYYMLPMWYMAEDRLAW
                     WDKFSQPAVRPVYSLGIDTWWYDVNKATKLPSARQQGE"
     gene            23370..24464
                     /locus_tag="J1Y83_000364"
     CDS             23370..24464
                     /locus_tag="J1Y83_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin C ABC transporter permease YejB"
                     /protein_id="HBA7628551.1"
                     /translation="MGAYLIRRLLLVVPTLWAIITINFFIVQIAPGGPVDQAIAAIEF
                     GNAGVLPGAGGEGVRTSHAQTGVGNISDSNYRGGRGLDPEVIAEITHRYGFDKPIHER
                     YFKMLWDYIRFDFGDSLFRSASVLTLIKDSLPVSITLGLWSTLIIYLVSIPLGIRKAV
                     YNGSRFDVWSSAFIIIGYAIPAFLFAILLIVFFAGGSYFDLFPLRGLVSANFDSLPWY
                     QKITDYLWHITLPVLATVIGGFAALTMLTKNSFLDEVRKQYVVTARAKGVSEKNILWK
                     HVFRNAMLLVIAGFPATFISMFFTGSLLIEVMFSLNGLGLLGYEATVSRDYPVMFGTL
                     YIFTLIGLLLNIVSDISYTLVDPRIDFEGR"
     gene            24464..25489
                     /locus_tag="J1Y83_000365"
     CDS             24464..25489
                     /locus_tag="J1Y83_000365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBA7628552.1"
                     /translation="MSRLSPVNQARWARFRHNRRGYWSLWIFLVLFGLSLCSELIAND
                     KPLLVRYDGSWYFPLLKNYSESDFGGPLASQADYQDPWLKQRLENNGWVLWAPIRFGA
                     TSINFATDKPFPSPPSRQNWLGTDANGGDVLARILYGTRISVLFGLMLTLCSSVMGVL
                     AGALQGYYGGKVDLWGQRFIEVWSGMPTLFLIILLSSVVQPNFWWLLAITVLFGWMSL
                     VGVVRAEFLRTRNFDYIRAAQALGVSDRSIILRHMLPNAMVATLTFLPFILCSSITTL
                     TSLDFLGFGLPLGSPSLGELLLQGKNNLQAPWLGITAFLSVAILLSLLIFIGEAVRDA
                     FDPNKAV"
     gene            25491..27080
                     /gene="yejF"
                     /locus_tag="J1Y83_000366"
     CDS             25491..27080
                     /gene="yejF"
                     /locus_tag="J1Y83_000366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708079.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin C ABC transporter ATP-binding protein
                     YejF"
                     /protein_id="HBA7628553.1"
                     /translation="MTQTLLAIENLSVGFRHQQTVRTVVNDVSLQIAAGETLALVGES
                     GSGKSVTALSILRLLPCPPVEYLSGDIRFHGESLLHASDQTLRGVRGNKIAMIFQEPM
                     VSLNPLHTLEKQLYEVLSLHRGMRREAARGEILNCLDRVGIRQAAKRLTDYPHQLSGG
                     ERQRVMIAMALLTRPELLIADEPTTALDVSVQAQILQLLRELQGELNMGMLFITHNLS
                     IVRKLAHRVAVMQNGRCVEKNNAATLFASPTHPYTQKLLNSEPSGDPVPLPEPASTLL
                     DVEQLQVAFPIRKGILKRIVDHNVVVKNISFTLRAGETLGLVGESGSGKSTTGLALLR
                     LIDSQGSIVFDGQPLQNLNRRQLLPIRHRIQVVFQDPNSSLNPRLNVLQIIEEGLRVH
                     QPTLSAAQREQQVIAVMHEVGLDPETRHRYPAEFSGGQRQRIAIARALILKPSLIILD
                     EPTSSLDKTVQAQILTLLKSLQQKHQLAYLFISHDLHVVRALCHQVIVLRQGEVVEQG
                     PCARVFAAPQQEYTRQLLALS"
     gene            complement(27084..27428)
                     /locus_tag="J1Y83_000367"
     CDS             complement(27084..27428)
                     /locus_tag="J1Y83_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA7628554.1"
                     /translation="MTPLQLSIVHRLPQNYRWSAGFAGSKVEPIPQNGPCGDNSLVAL
                     KLLSPDGDNAWSVMYKLSQALSDIEVPCSVLECEGEPCLFVNRQDEFAATCRLKNFGV
                     AIAEPFSNYNPF"
     gene            complement(27761..28951)
                     /gene="bcr"
                     /locus_tag="J1Y83_000368"
     CDS             complement(27761..28951)
                     /gene="bcr"
                     /locus_tag="J1Y83_000368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter Bcr"
                     /protein_id="HBA7628555.1"
                     /translation="MTTRQHSSFAIVFILGLLAMLMPLSIDMYLPALPVISAQFGVPA
                     GSTQMTLSTYILGFALGQLIYGPMADSFGRKPVVLGGTLVFAAAAVACALAQTIDQLI
                     VMRFFHGLAAAAASVVINALMRDIYPKEEFSRMMSFVMLVTTIAPLMAPIVGGWVLVW
                     LSWHYIFWILAVAAILASAMIFFLIKETLPPERRQPFHIRTTIGNFAALFRHKRVLSY
                     MLASGFSFAGMFSFLSAGPFVYIEINHVAPENFGYYFALNILFLFVMTIFNSRFVRRI
                     GALNMFRSGLWIQFIMAAWMVISALLGLGFWSLVVGVAAFVGCVSMVSSNAMAVILDE
                     FPHMAGTASSLAGTFRFGIGAIVGALLSLATFNSAWPMIWSIAFCATSSILFCLYASR
                     PKKR"
     gene            complement(28979..29674)
                     /gene="rsuA"
                     /locus_tag="J1Y83_000369"
     CDS             complement(28979..29674)
                     /gene="rsuA"
                     /locus_tag="J1Y83_000369"
                     /EC_number="5.4.99.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA pseudouridine(516) synthase RsuA"
                     /protein_id="HBA7628556.1"
                     /translation="MRLDKFIAQQLGVSRAIAGREIRGNRVTVDGEIVRNAAFKLLPE
                     HDVAYDGNPLAQQHGPRYFMLNKPQGYVCSTDDPDHPTVLYFLDEPVAWKLHAAGRLD
                     IDTTGLVLMTDDGQWSHRITSPRHHCEKTYLVTLESPVADDTAEQFAKGVQLHNEKDL
                     TKPAVLEVITPTQVRLTISEGRYHQVKRMFAAVGNHVVELHRERIGGITLDADLAPGE
                     YRPLTEEEIASVV"
     gene            29824..31584
                     /locus_tag="J1Y83_000370"
     CDS             29824..31584
                     /locus_tag="J1Y83_000370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HBA7628557.1"
                     /translation="MIFTLRPYQQEAVDATLNHFRRHKTPAVIVLPTGAGKSLVIAEL
                     ARLARGRVLVLAHVKELVAQNHAKYQALGLEADIFAAGLKRKESHGKVVFGSVQSVAR
                     NLDAFQGEFSLLIVDECHRIGDDEESQYQQILTHLTKVNPHLRLLGLTATPFRLGKGW
                     IYQFHYHGMVRGDEKALFRDCIYELPLRYMIKHGYLTPPERLDMPVVQYDFSRLQAQS
                     NGLFSEADLNRELKKQQRITPHIISQIMEFAATRKGVMIFAATVEHAKEIVGLLPAED
                     AALITGDTPGAERDVLIEDFKAQRFRYLVNVAVLTTGFDAPHVDLIAILRPTESVSLY
                     QQIVGRGLRLAPGKTDCLILDYAGNPHDLYAPEVGTPKGKSDNVPVQVFCPACGFANT
                     FWGKTTADGTLIEHFGRRCQGWFEDDDGHREQCDFRFRFKNCPQCNAENDIAARRCRE
                     CDTILVDPDDMLKAALRLKDALVLRCSGMSLQHGHDEKGEWLKITYYDEDGADVSERF
                     RLQTPAQRTAFEQLFIRPHTRTPGIPLRWITAADILAQQALLRHPDFVVARMKGQYWQ
                     VREKVFDYEGRFRRAHELRG"
     gene            31709..31993
                     /gene="rplY"
                     /locus_tag="J1Y83_000371"
     CDS             31709..31993
                     /gene="rplY"
                     /locus_tag="J1Y83_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000494192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HBA7628558.1"
                     /translation="MFTINAEVRKEQGKGASRRLRAANKFPAIIYGGKEAPLAVELDH
                     DKVMNMQVKAEFYSEVLTIVVDGKEIKVKAQDVQRHPYKPKLLHIDFVRA"
     gene            complement(32132..33139)
                     /gene="yejK"
                     /locus_tag="J1Y83_000372"
     CDS             complement(32132..33139)
                     /gene="yejK"
                     /locus_tag="J1Y83_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein YejK"
                     /protein_id="HBA7628559.1"
                     /translation="MSLDINQIALHQLIKRDEQNLELVLRDSLLEPTETVVEMVAELH
                     RVYSAKNKAYGLFSEESELAQTLRLQRQGEEDFLAFSRAATGRLRDELAKYPFADGGF
                     VLFCHYRYLAVEYLLVAVLSNLSSMRVNENLDINPTHYLDINHADIVARIDLTEWETN
                     PESTRYLTFLKGRVGRKVADFFMDFLGASEGLNAKAQNRGLLQAVDDFTAEAQLDKAE
                     RQNVRQQVYSYCNEQLQAGEEIELESLSKELAGVSEVSFTEFAAEKGYELEESFPADR
                     STLRQLTKFAGSGGGLTINFDAMLLGERIFWDPATDTLTIKGTPPNLRDQLQRRTSGG
                     N"
     gene            33321..33548
                     /locus_tag="J1Y83_000373"
     CDS             33321..33548
                     /locus_tag="J1Y83_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YejL family protein"
                     /protein_id="HBA7628560.1"
                     /translation="MPQISRYSDEQVEQLLAELLNILEKHKAPTDLSLMVLGNMVTNL
                     INTSIAPAQRQAIANSFARALQSSINEDKAH"
     gene            33568..35328
                     /gene="pbgA"
                     /locus_tag="J1Y83_000374"
     CDS             33568..35328
                     /gene="pbgA"
                     /locus_tag="J1Y83_000374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin transport protein PbgA"
                     /protein_id="HBA7628561.1"
                     /translation="MVTHRQRYREKVSQMVSWGHWFALFNILLSLVIGSRYLFIADWP
                     TTLAGRIYSYVSIIGHFSFLVFATYLLILFPLTFIVGSQRLMRFLSVILATAGMTLLL
                     IDSEVFTRFHLHLNPIVWQLVINPDENEMARDWQLMFISVPVILLLELVFATWSWQKL
                     RSLTRRRRFARPLAAFLFIAFIASHVVYIWADANFYRPITMQRANLPLSYPMTARRFL
                     EKHGLLDAQEYQRRLIEQGNPDAVSVQYPLSELRYRDMGTGQNVLLITVDGLNYSRFE
                     KQMPALAGFAEQNISFTRHMSSGNTTDNGIFGLFYGISPSYMDGILSTRTPAALITAL
                     NQQGYQLGLFSSDGFTSPLYRQALLSDFSMPSVRTQSDEQTATQWINWLGRYAQEDNR
                     WFSWVSFNGTNIDDSNQQAFARKYSRAAGNVDDQINRVLNALRDSGKLDNTVVIITAG
                     RGIPLSEEEETFDWSHGHLQVPLVIHWPGTPAQRINALTDHTDLMTTLMQRLLHVSTP
                     ASEYSQGQDLFNPQRRHYWVTAADNDTLAITTPKKTLVLNNNGKYRTYNLRGERVKDE
                     KPQLSLLLQVLTDEKRFIAN"
     gene            35403..35479
                     /locus_tag="J1Y83_000375"
     tRNA            35403..35479
                     /locus_tag="J1Y83_000375"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:35437..35439,aa:Pro,seq:ggg)
     gene            complement(35581..36066)
                     /locus_tag="J1Y83_000376"
     CDS             complement(35581..36066)
                     /locus_tag="J1Y83_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001607745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HBA7628562.1"
                     /translation="MGGAVNQTTINNGVLQVYGAANDPTIKGGRGDAAFTLGNAGSVV
                     DISTYEYTLLDNGNHSWSLAENRVQMPPSTTDVLNMAAAQPLVFDAELDTVRGRLGSV
                     KGVNYDTAMWSSAINSRNSVNTDAGAGFEQTLTGLTLGIDSRFSCEESSTTQAGVVWT
                     F"
     gene            complement(36175..36378)
                     /locus_tag="J1Y83_000377"
     CDS             complement(36175..36378)
                     /locus_tag="J1Y83_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001323532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA7628563.1"
                     /translation="MHQSGSVSLCRSAISVLVATALYSPIALASTVEYGETVDGVVLE
                     KDIQLVYGAANNTKINPGGEQHI"
     gene            36698..37345
                     /gene="narP"
                     /locus_tag="J1Y83_000378"
     CDS             36698..37345
                     /gene="narP"
                     /locus_tag="J1Y83_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite response regulator protein NarP"
                     /protein_id="HBA7628564.1"
                     /translation="MPEATPFQVMIVDDHPLMRRGVRQLLELDSGFEVVAEAGDGASA
                     IDLANRLDIDVILLDLNMKGMSGLDTLNALRRDGVTAQIIILTVSDASSDVFALIDAG
                     ADGYLLKDSDPEVLLEAIRAGAKGSKVFSERVNQYLREREMFGAEEDPFSVLTERELD
                     VLHELAQGLSNKQIASVLNISEQTVKVHIRNLLRKLNVRSRVAATILFLQQRGAQ"
     gene            complement(37380..38432)
                     /locus_tag="J1Y83_000379"
     CDS             complement(37380..38432)
                     /locus_tag="J1Y83_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis protein CcmH"
                     /protein_id="HBA7628565.1"
                     /translation="MRFLLGVLMLMISGSALATIDVLQFKDEAQEQQFRQLTEELRCP
                     KCQNNSIADSNSMIATDLRQKVYELMQEGKSKKEIVDYMVARYGNFVTYDPPLTPLTV
                     LLWVLPVVAIGIGVWVIYARSRRRVRVVPEAFPEQSVPEGKRAGYVVYLPGIVVALIV
                     AGVSYYQTGNYQQVKIWQQATAQAPALLDRALDPKADPLNEEEMSRLALGMRTQLQKN
                     PGDIEGWIMLGRVGMALGNASIATDAYATAYRLDPKNSDAALGYAEALTRSSDPNDNR
                     LGGELLRQLVRTDHSNIRVLSMYAFNAFEQQRFGEAVAAWEMMLKLLPANDTRRAVIE
                     RSIAQAMQHLSPQESK"
     gene            complement(38429..38986)
                     /gene="dsbE"
                     /locus_tag="J1Y83_000380"
     CDS             complement(38429..38986)
                     /gene="dsbE"
                     /locus_tag="J1Y83_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbE"
                     /protein_id="HBA7628566.1"
                     /translation="MKRKVLLIPLIIFLAIAAALLWQLARNAEGDDPTNLESALIGKP
                     VPKFRLESLDNPGQFYQADVLTQGKPVLLNVWATWCPTCRAEHQYLNQLSAQGIRVVG
                     MNYKDDRQKAISWLKELGNPYALSLFDGDGMLGLDLGVYGAPETFLIDGNGIIRYRHA
                     GDLNPRVWEEEIKPLWEKYSKEAAQ"
     gene            complement(38983..40926)
                     /locus_tag="J1Y83_000381"
     CDS             complement(38983..40926)
                     /locus_tag="J1Y83_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000982423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase CcmF/NrfE family subunit"
                     /protein_id="HBA7628567.1"
                     /translation="MMPEIGNGLLCLALGIALLLSVYPLWGVARGDARMMASSRLFAW
                     LLFMSVAGAFLVLVNAFVVNDFTVTYVASNSNTQLPVWYRVAATWGAHEGSLLLWVLL
                     MSGWTFAVAIFSQRIPLDIVARVLAIMGMVSVGFLLFILFTSNPFSRTLPNFPIEGRD
                     LNPLLQDPGLIFHPPLLYMGYVGFSVAFAFAIASLLSGRLDSTYARFTRPWTLAAWIF
                     LTLGIVLGSAWAYYELGWGGWWFWDPVENASFMPWLVGTALMHSLAVTEQRASFKAWT
                     LLLAISAFSLCLLGTFLVRSGVLVSVHAFASDPARGMFILAFMVLVIGGSLLLFAARG
                     HKVRSRVNNALWSRESLLLANNVLLVAAMLVVLLGTLLPLVHKQLGLGSISIGEPFFN
                     TMFTWLMVPFALLLGVGPLVRWGRDRPRKIRNLLIIAFISTLVLSLLLPWLFESKVVA
                     MTVLGLAMACWIAVLAIAEAALRISRGTKTTFSYWGMVAAHLGLAVTIVGIAFSQNYS
                     VERDVRMKSGDSVDIHEYRFTFRDVKEVTGPNWRGGVATIGVTRDGKPETVLYAEKRY
                     YNTAGSMMTEAAIDGGITRDLYAALGEELENGAWAVRLYYKPFVRWIWAGGLMMALGG
                     LLCLFDPRYRKRVNPQKTAPEAA"
     gene            complement(40923..41402)
                     /gene="ccmE"
                     /locus_tag="J1Y83_000382"
     CDS             complement(40923..41402)
                     /gene="ccmE"
                     /locus_tag="J1Y83_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c maturation protein CcmE"
                     /protein_id="HBA7628568.1"
                     /translation="MNIRRKNRLWIACAVLAGLALTIGLVLYALRSNIDLFYTPGEIL
                     YGKRETQQMPEVGQRLRVGGMVMPGSVQRDPNSLKVTFTIYDAEGSVDVSYEGILPDL
                     FREGQGVVVQGELEKGNHILAKEVLAKHDENYTPPEVEKAMEANHRRPASVYKDPAS"
     gene            complement(41399..41608)
                     /gene="ccmD"
                     /locus_tag="J1Y83_000383"
     CDS             complement(41399..41608)
                     /gene="ccmD"
                     /locus_tag="J1Y83_000383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmD"
                     /protein_id="HBA7628569.1"
                     /translation="MTPAFASWNEFFAMGGYAFFVWLAVVMTVIPLVVLVVYSVMQHR
                     AILRGVAQQRAREARLRAAQQQEAA"
     gene            complement(41605..42342)
                     /gene="ccmC"
                     /locus_tag="J1Y83_000384"
     CDS             complement(41605..42342)
                     /gene="ccmC"
                     /locus_tag="J1Y83_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmC"
                     /protein_id="HBA7628570.1"
                     /translation="MWKTLHQLAIPPRLYQICGWFIPWLAIASVVVLTVGWIWGFGFA
                     PADYQQGNSYRIIYLHVPAAIWSMGIYASMAVAAFIGLVWQMKMANLAVAAMAPIGAV
                     FTFIALVTGSAWGKPMWGTWWVWDARLTSELVLLFLYVGVIALWHAFDDRRLAGRAAG
                     ILVLIGVVNLPIIHYSVEWWNTLHQGSTRMQQSIDPAMRSPLRWSIFGFLLLSATLTL
                     MRMRNLILLMEKRRPWVSELILKRGRK"
     gene            complement(42384..43046)
                     /gene="ccmB"
                     /locus_tag="J1Y83_000385"
     CDS             complement(42384..43046)
                     /gene="ccmB"
                     /locus_tag="J1Y83_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmB"
                     /protein_id="HBA7628571.1"
                     /translation="MMFWRIFRLELRVAFRHSAEIANPLWFFLIVITLFPLSIGPEPQ
                     LLARIAPGIIWVAALLSSLLALERLFRDDLQDGSLEQLMLLPLPLPAVVLAKVMAHWM
                     VTGLPLLILSPLVAMLLGMDVYGWQVMALTLLLGTPTLGFLGAPGVALTVGLKRGGVL
                     LSILVLPLTIPLLIFATAAMDAASMHLPVDGYLAILGALLAGTATLSPFATAAALRIS
                     IQ"
     gene            complement(43043..43660)
                     /gene="ccmA"
                     /locus_tag="J1Y83_000386"
     CDS             complement(43043..43660)
                     /gene="ccmA"
                     /locus_tag="J1Y83_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001300065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis heme-transporting ATPase
                     CcmA"
                     /protein_id="HBA7628572.1"
                     /translation="MLEARELLCERDERTLFSGLSFTLNAGEWVQITGSNGAGKTTLL
                     RLLTGLSRPDAGDVLWQGQPLHQVRDSYHQNLLWIGHQPGIKTRLTALENLHFYHRDG
                     DTAQCLEALAQAGLAGFEDIPVNQLSAGQQRRVALARLWLTRATLWILDEPFTAIDVN
                     GVDRLTQRMAQHTEQGGIVILTTHQPLNVAESKIRRISLTQTGAA"
     gene            complement(43679..44281)
                     /gene="napC"
                     /locus_tag="J1Y83_000387"
     CDS             complement(43679..44281)
                     /gene="napC"
                     /locus_tag="J1Y83_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type protein NapC"
                     /protein_id="HBA7628573.1"
                     /translation="MGNSDRKPGLIKRLWKWWRTPSRLALGTLLLIGFVGGIVFWGGF
                     NTGMEKANTEEFCISCHEMRNTVYQEYMDSVHYNNRSGVRATCPDCHVPHEFVPKMIR
                     KLKASKELYGKIFGVIDTPQKFEAHRLTMAQNEWRRMKDNNSQECRNCHNFEYMDTTA
                     QKSVAAKMHDQAVKDGQTCIDCHKGIAHKLPDMREVEPGF"
     gene            complement(44291..44740)
                     /gene="napB"
                     /locus_tag="J1Y83_000388"
     CDS             complement(44291..44740)
                     /gene="napB"
                     /locus_tag="J1Y83_000388"
                     /EC_number="1.9.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase cytochrome c-type subunit"
                     /protein_id="HBA7628574.1"
                     /translation="MKSHDLKKALCQWTAMLALVVSGAVWAANGVDFSQSPEVSGTQE
                     GAIRMPKEQDRMPLNYVNQPPMIPHSVEGYQVTTNTNRCLQCHGVESYRTTGAPRISP
                     THFMDSDGKVGAEVAPRRYFCLQCHVPQADTAPIVGNTFTPSKGYGK"
     gene            complement(44737..45600)
                     /gene="napH"
                     /locus_tag="J1Y83_000389"
     CDS             complement(44737..45600)
                     /gene="napH"
                     /locus_tag="J1Y83_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinol dehydrogenase ferredoxin subunit NapH"
                     /protein_id="HBA7628575.1"
                     /translation="MANRKRDAGREALEKKGWWRSHRWLVLRRLCQFFVLGMFLSGPW
                     FGVWILHGNYSSSLLFDTVPLTDPLMTLQSLASGHLPATVALTGAVIITVLYALAGKR
                     LFCSWVCPLNPITDLANWLRRRFDLNQSATIPRHIRYVLLVVILVGSALTGTLIWEWI
                     NPVSLMGRSLVMGFGSGALLILALFLFDLLVVEHGWCGHICPVGALYGVLGSKGVITV
                     AATDRQKCNRCMDCFHVCPEPHVLRAPVLDEQSPVQVTSRDCMTCGRCVDVCSEDVFT
                     ITTRWSSGAKS"
     gene            complement(45587..46282)
                     /gene="napG"
                     /locus_tag="J1Y83_000390"
     CDS             complement(45587..46282)
                     /gene="napG"
                     /locus_tag="J1Y83_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-type protein NapG"
                     /protein_id="HBA7628576.1"
                     /translation="MSRSAKPQNGRRRFLRDVVRTAGGLAAVGVALGLQQQTARASGV
                     RLRPPGAINENAFASACVRCGQCVQACPYDTLKLATLASGLSAGTPYFVARDIPCEMC
                     EDIPCAKVCPSGALDREIESIDDARMGLAVLVDQENCLNFQGLRCDVCYRECPKIDEA
                     ITLELERNTRTGKHARFLPTVHSDACTGCGKCEKVCVLEQPAIKVLPLSLAKGELGHH
                     YRFGWLEGNNGKS"
     gene            complement(46289..48775)
                     /gene="napA"
                     /locus_tag="J1Y83_000391"
     CDS             complement(46289..48775)
                     /gene="napA"
                     /locus_tag="J1Y83_000391"
                     /EC_number="1.7.99.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="periplasmic nitrate reductase subunit alpha"
                     /protein_id="HBA7628577.1"
                     /translation="MKLSRRSFMKANAVAAAAAAAGLSVPGVARAVVGQQEAIKWDKA
                     PCRFCGTGCGVLVGTQQGRVVACQGDPDAPVNRGLNCIKGYFLPKIMYGKDRLTQPLL
                     RMKNGKYDKEGEFTPITWDQAFDVMEEKFKTALKEKGPESIGMFGSGQWTIWEGYAAS
                     KLFKAGFRSNNIDPNARHCMASAVVGFMRTFGMDEPMGCYDDIEQADAFVLWGSNMAE
                     MHPILWSRITNRRLSNQNVTVAVLSTYQHRSFELADNGIIFTPQSDLVILNYIANYII
                     QNNAINQDFFSKHVNLRKGATDIGYGLRPTHPLEKAAKNPGSDASEPMSFEDYKAFVA
                     EYTLEKTAEMTGVPKDQLEQLAQLYADPNKKVISYWTMGFNQHTRGVWANNLVYNLHL
                     LTGKISQPGCGPFSLTGQPSACGTAREVGTFAHRLPADMVVTNEKHRDICEKKWNIPS
                     GTIPAKIGLHAVAQDRALKDGKLNVYWTMCTNNMQAGPNINEERMPGWRDPRNFIIVS
                     DPYPTVSALAADLILPTAMWVEKEGAYGNAERRTQFWRQQVQAPGEAKSDLWQLVQFS
                     RRFKTEEVWPEELLAKKPELRGKTLYDVLYATPEVSKFPVSELAEDQLNDESRELGFY
                     LQKGLFEEYAWFGRGHGHDLAPFDDYHKARGLRWPVVNGKETQWRYSEGNDPYVKAGE
                     GYKFYGKPDGKAVIFALPFEPAAEAPDEEYDLWLSTGRVLEHWHTGSMTRRVPELHRA
                     FPEAVLFIHPLDAKARDLRRGDKVKVVSRRGEVISIVETRGRNRPPQGLVYMPFFDAA
                     QLVNKLTLDATDPLSKETDFKKCAVKLEKV"
     gene            complement(48772..49035)
                     /gene="napD"
                     /locus_tag="J1Y83_000392"
     CDS             complement(48772..49035)
                     /gene="napD"
                     /locus_tag="J1Y83_000392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone NapD"
                     /protein_id="HBA7628578.1"
                     /translation="MHTNWQVCSLVVQAKSERISDISTQLNAFTGCEVAVSDAPSGQL
                     IVVVEAEDSETLIQTIESVRNVEGVLAVSLVYHQQEEQGEETP"
     gene            complement(49025..49519)
                     /gene="napF"
                     /locus_tag="J1Y83_000393"
     CDS             complement(49025..49519)
                     /gene="napF"
                     /locus_tag="J1Y83_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-type protein NapF"
                     /protein_id="HBA7628579.1"
                     /translation="MKIDASRRGILTGRWRKASNGIRPPWSGDESHFLTHCTRCDACI
                     NACENNILQRGAGGYPSVNFKNNECSFCYACAQACPESLFSPRHTRAWDLQFTIGDAC
                     LAYQSVECRRCQDSCEPMAIIFRPTLSGIYQPQLNSQLCNGCGACAASCPVSAITAEY
                     LHAH"
     gene            49628..49792
                     /locus_tag="J1Y83_000394"
     CDS             49628..49792
                     /locus_tag="J1Y83_000394"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA7628580.1"
                     /translation="MHTPIGVKPVAGSKEWREAWQKRAFAHISNGYKHIYIAINSPEI
                     FLLVCFLIRI"
     gene            49927..50415
                     /gene="eco"
                     /locus_tag="J1Y83_000395"
     CDS             49927..50415
                     /gene="eco"
                     /locus_tag="J1Y83_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease inhibitor ecotin"
                     /protein_id="HBA7628581.1"
                     /translation="MKTILPAVLFAAFATTSAWAAESVQPLEKIAPYPQAEKGMKRQV
                     IQLTPQEDESTLKVELLIGQTLEVDCNLHRLGGKLESKTLEGWGYDYYVFDKVSSPVS
                     TMMACPDGKKEKKFVTAYLGDAGMLRYNSKLPIVVYTPDNVDVKYRVWKAEEKIDNAV
                     VR"
     gene            complement(50565..52211)
                     /gene="mqo"
                     /locus_tag="J1Y83_000396"
     CDS             complement(50565..52211)
                     /gene="mqo"
                     /locus_tag="J1Y83_000396"
                     /EC_number="1.1.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase (quinone)"
                     /protein_id="HBA7628582.1"
                     /translation="MKKVTAMLFSMAVGLNAVSMAAKAKASEEQETDVLLIGGGIMSA
                     TLGTYLRELEPEWSMTMVERLEGVAQESSNGWNNAGTGHSALMELNYTPQNADGSISI
                     EKAVAINEAFQISRQFWAHQVERGVLRTPRSFINTVPHMSFVWGEDNVNFLRARYAAL
                     QQSSLFRGMRYSEDHAQIKEWAPLVMEGRDPQQKVAATRTEIGTDVNYGEITRQLIAS
                     LQKKSNFSLQLSSEVRALKRNDDNSWTVTVADLKNGTAQNIRAKFVFIGAGGAALKLL
                     QESGIPEAKDYAGFPVGGQFLVSENPDVVNHHLAKVYGKASVGAPPMSVPHIDTRVLD
                     GKRVVLFGPFATFSTKFLKNGSLWDLMSSTTTSNVMPMMHVGLDNFDLVKYLVSQVML
                     SEEDRFEALKEYYPQAKKEDWRLWQAGQRVQIIKRDADKGGVLRLGTEVVSDQQGTIA
                     ALLGASPGASTAAPIMLNLLEKVFGDRVSSPQWQATLKAIVPSYGRKLNGDVAATERE
                     LQYTSEVLGLKYDKPQAADSTPKPQLKPQPVQKEVADIAL"
BASE COUNT        12784 a        14038 c        12883 g        12603 t
ORIGIN      
        1 atatataatc aattttattt ttaaaccagt tagtcgtaac cttatgcccg gttaaaggag
       61 agggttatga cgaatatcac cttacagaaa caacatcgta cactgtggca ttttattccg
      121 gggttagccc tgagtgcagt tatcaccggg gtcgccctgt ggggtggttc cattcccgcg
      181 gttgcgggtg ccgggtttag tgccctcacc ctcgcaatct tgttggggat ggttttaggc
      241 aacaccattt acccgcacat ctggaaaagc tgtgacggtg gtgtgctgtt tgccaagcaa
      301 tatttattac gtctgggtat cattctttat ggcttccgtc tgacgttctc gcaaattgcc
      361 gatgtcggta tcagtgggat catcattgac gtgttgacgc tgtccagtac cttcctgctg
      421 gcttgcttcc tggggcagaa agtgtttggt ctggataagc acaccagctg gttgatcggt
      481 gccggtagca gtatctgtgg tgctgccgcg gtactggcga ctgagccggt agtaaaagca
      541 gaagccagta aagtaaccgt ggctgttgca accgttgtta tcttcgggac cgtcgcgatt
      601 ttcctctacc cggcgatata tccgctgatg tctcaatggt ttagtccgga aactttcggt
      661 atctacattg gttctactgt gcacgaagtg gcgcaggtgg tggcggcagg tcatgccatc
      721 agcccggatg cagaaaacgc agcagttatt tccaaaatgc tgcgcgtgat gatgctggct
      781 ccgttcctca tcctgctggc ggcgcgtgtt aaacagctgt ctggggcgaa cagcggcgag
      841 aaaagcaaaa tcactattcc gtggtttgcc atcttgttca tcgtcgttgc catctttaac
      901 tcgttccacc tgttaccgca gagcgtggtg aacatgctgg taacgctgga taccttcctg
      961 ctggcaatgg cgatggcggc gctgggtctg accactcacg tcagcgcgct gaaaaaagcc
     1021 ggggcgaaac ctctgctgat ggcactggta ctgtttgcct ggctgattgt tggtggtggt
     1081 gcgattaact atgtgattca aagcgtcatc gcataaacca ctacatcttg ctcctgttaa
     1141 cccgctatca ttaccgtttt cctccagcgg gtttaacagg agtcctcgca tgaaatacat
     1201 tggagcgcac gttagtgctg ctggcggtct ggcaaatgcc gcaattcgcg ccgccgaaat
     1261 cgacgcaacc gcgtttgcct tgttcaccaa aaaccaacgt cagtggcgtg ccgcaccgct
     1321 cacgacgcaa accatcgatg aattcaaagc cgcctgtgaa aaatatcact acacatcggc
     1381 gcaaattctt ccccacgaca gttatctgat taacctcgga catccggttg ctgaagctct
     1441 ggaaaaatcg cgcgatgcct ttatagatga aatgcagcgt tgcgaacaac tgggactttc
     1501 tttgctcaac ttccaccctg gcagccatct gatgcagatt tcagaagagg attgccttgc
     1561 gcgtattgcc gaatccatca acattgcgct ggataaaact caaggtgtca ccgcggtgat
     1621 tgaaaacacc gccggtcagg gcagtaactt agggtttaaa ttcgaacatc tcgcagcgat
     1681 tatcgacggc gtggaagata aatcccgcgt cggcgtctgc attgatacct gccatgcttt
     1741 cgctgccggg tatgatttgc gtactcctgc cgaatgcgag aaaacgttcg ctgattttgc
     1801 ccgtattgtt ggctttaagt atctgcgcgg gatgcacctc aacgatgcga aaagcacctt
     1861 tggcagccgc gttgaccgtc atcatagcct cggcgaaggc aatatcggtc atgatgcgtt
     1921 ccgctggatc atgcaagacg accgtttcga cggcattccg ctgatccttg aaaccatcaa
     1981 cccggatatc tgggcagaag agatcgcctg gctgaaagcg caacaaactg aaaaagcggt
     2041 ggcctgatcg atgaacgaca gggaaaagca gattcttaaa attttacggc gtaacccgct
     2101 gattcaacag aatgaaatcg ccgatattct gcaaatcagc cgctcacgtg ttgccgctca
     2161 tattatggat ttgatgcgca agggtttgat taaaggcaaa ggatatatcc ttaccgagca
     2221 ggattactgt gtggttgtgg gtgcaatcaa tatggatatt cgcggaatgg cggatatccg
     2281 ttacccgcaa gcagcctctc atccaggttc tgttcactgt tccgcaggcg gcgtcggacg
     2341 taatatcgcg cataacctgg ctctgcttgg tcgggatgtg catttaattt cagctattgg
     2401 caacgatttt tacggcgaga cattactgga agaaacgcgc cgcgcgggcg tgaacgtttc
     2461 aaactgcatt cgtctgcatg gacacagcac tgcaacttat ctcgctatcg ctaataagca
     2521 ggaagaaact atcctggcaa tcaatgacac acatattctg caacagctaa cgcctcaact
     2581 gctcaatact tcacgggact tgattcgcca tgctggtgtg gtgctggctg attgtaatct
     2641 cacacctgaa gccctggagt gggtctttac cattgctgat gaaattccga tgtttgtcga
     2701 taccgtttcc gaatttaaag cgaacaaagt aaaaaactgg tacagccgga tccacactct
     2761 gaaaccgaca caaaatgaac tggaaatttt gtgggggcaa ccgattaaag acgacaacga
     2821 tcgcatcaga gctgtaaatt cgctgcatca acaaggtgtt aaacgcattt ttgtttatct
     2881 gaaagatgaa tctgtatttt gcagcgataa ggatggagaa aaatttttgc tgaccgcgcc
     2941 agcgcatacg acagtagaca gttttggtgc tgacgatggt tttatggcgg gcctggtata
     3001 tagctttctt gaaggataca gtttccgcga cagcgcccgt tttgcgatgg cctgcgcggc
     3061 aatttcacgc gccagcggca gcttaaacaa ccctaccctg tctgccgata acgcgctttc
     3121 attagtgcca atggtgtaac aatgttgccg gattcgacgc ttgaccgcat cttatccggc
     3181 tcataaattg cgcactacgc taaaccaata aagaatcctg caatagtagc actcatcagg
     3241 ttactgacag caaaaacgca atagccagta ataccaccat tcccagaaca cttctcatga
     3301 catccatagt tattatctct tcatgtcatt ttgggcgacg gctctctgcc catcataaat
     3361 agccgggtct gcatcttgcg atactgaccc ggcttgttat caacacttaa tgggttttta
     3421 tgtagccgcg tacgcactct tcgactaatc cccagaacca gtcggtatca atagtgatgc
     3481 cgactttggt attggcgggc ttgccaagaa cgcccagctc gtcgcagacg gtacgcccat
     3541 agcaagggcc actgttaacg tccacttcga cgtacatctc ctgggtttta atgccatcat
     3601 ggttaatcaa ataaccgatg caggtggcgt cgtgcaccgg gccgccagcc aggccgtagt
     3661 tttcgaactg cgttttgaga gtgaagttca tgatgtcgct gaacagctct ccggcagggc
     3721 cgccagccct ttccatccga gcaatcacat ccggggtgca aacggtctgg ttggtaagat
     3781 cgaggcccat catcactaat ggaacgccgg aggtgaacac cacgcgtgcg gcctccgggt
     3841 cagcaaagat gttgaactca gcagatgggg tgaagttgcc agtaccataa gcgccgccca
     3901 tcagtacaat ttcacggatt tttggcagga tcgcgggctg catacgcatt gccaccgcga
     3961 tatttgaaag cggaccaacc ggcaccagag tgatatcgcc atcgcttgcc ataagggtat
     4021 cgatgatata tttcaccgca tgagtgtttt ctgcctggcg ggtcagcggc tcgaataccg
     4081 ggccatccag tccggtttca ccgtgaatat tatcggcaac aatttgttga cgcataatgg
     4141 gctgcggcat ccccgcataa accggaacat taatctccag tttctggcaa acattcaggc
     4201 cattaattaa tgttttatcg agggtttgat tacccgctac aatggtgata cctaataaat
     4261 caatcgccgg atgtttcgcc gccatcatta tagcaatagc atcatcatga cccggatcac
     4321 aatccagaat aatttttctc ttttccattg tttatttcct ctgtttccag ttgcgttatt
     4381 gtttctacag caaagaaaat tcgacctggc cgatgattga atcttaacaa cagcgtacgt
     4441 atgctaaata tgagaaatct catagcggat aaacatcgtg aaagaaatcc acaataatga
     4501 tcttaagcag caattgatga gtgaatctgc gtttaaggat tgcttttcaa cggatgtttc
     4561 agccgatacg cggctgtttc attttttagc gcgtgactac attgtgcagg aagggcaaca
     4621 gccgtcctgg ctgttttacc tgacgcgagg ccgcgccagg ctttacgtca cgcttgctaa
     4681 tggtcgcgtg tcgctgatcg atttctttgc cgcgccatgt tttattggcg agattgagtt
     4741 aatcgacaaa gaccatgaac cgcgtgcagt gcaggctatt gaagagtgtt ggtgccttgc
     4801 gctccctatg aagcattacc gcccgttgtt attaaacgac acgctatttt tacgaaaact
     4861 ctgcgtcacc ttaagtcata aaaattatcg taatattgtt tctttaactc agaatcaatc
     4921 atttccgtta gtgaatcgcc tggcagcttt tatattactc tcgcaggaag gtgatcttta
     4981 tcacgaaaag catacgcaag cggcagagta tttaggcgtt tcttatcgac atcttttata
     5041 tgttcttgcg caattcatta atgacggttt attaattaaa agcaagaaag ggtatctcat
     5101 taaaaacaga aagcagttgt caggactggc gctggagatg gacccggaga ataaattctc
     5161 cgggatgatg cagtaaaaat tattggcaat agtgcgattg ccggatgcaa cgcttaataa
     5221 ggcgtacatc cggcaatgaa actcaagcta aaccaataaa gaacccggca atggtcgcgc
     5281 tcatcaggtt agaaagtgtc gccgccgcca gcgcccgtaa accaagctgg gcgatttccg
     5341 gcgcgcgttt tggcgaaatg gccgaaaacg ctccaacgac aacaccgata gaaccaaagt
     5401 ttgcaaaccc acagagggcg aacgagataa tggcaatggt cttgacatcc agcgtgccgc
     5461 tcgtttgcag atagggcgag aaattgagat aagcaacaaa ctcattaatc gccagtttct
     5521 gcccaatcaa acttccggca agatttgcat cactccagtc cacccccata atccatgcca
     5581 acggggccag cacatagccg aaaatgcttt ccagagagac attggcaaaa ccaaaccagc
     5641 cgccaattcc gccgataatg ccgttgatca gcgcaataat tgcgacaaac gccatcacca
     5701 ccgtagccac accggctgca attttcagcc cggtcattgc accgttcgcc gcagcttcaa
     5761 taatgctttt tggcggtgtt tcgctaaacg acagattttc aaacgtaact tgcgaaggtt
     5821 ccgtcgccgg actaagaata cgtgcaaaca ggataccgcc aggaatagcc atcagcgaag
     5881 cggctaacaa atagtcaatc ggcacgccca ttccggcata gccaatcatc atcgaacccg
     5941 caatagacgc catgccgcta caaatagccg taaatagctc gttgcgattc atgcgatcaa
     6001 taaaaggttt cacgatcgcc gggatctcat tttgcccgag gaaaatagtg gtgactgcga
     6061 caaaagattc gattttgctg atgttgaggg ctttctgaaa aatactgcca agaatacgaa
     6121 tcagcagccc cattacgcca atgtagtaca gcaagctgat aagcgcggta acgaaaataa
     6181 tcgccgggag tacgcgaaag gcgaagataa agcccgcacc gtcaaacaga acatccattt
     6241 tgggaccgac cagcgaacca aaaataaagg cacttcctgc gtcactgtag gacatcactt
     6301 tatggacgcc taatgccgcc tgctctactg cccattttcc cgggggaaaa tagagcatga
     6361 tgccaccaat ggcaatttgc agcagcagcg cagccccaac cgtgcgcaag ctgatgctct
     6421 ttttatttac cgacaacaga aatgctattg ccagtaacac caccatcccc acaacacttc
     6481 tcattatatc cataatgatt ttcccttcat gccagtaaac ccggcgtcag cgccaggttt
     6541 tggtatgttt gatgagtacg ggcgacggct ttctgcccac cagaaattaa cccacgagac
     6601 gctgatattc tttggcaatt tcgctcgcca gaatggcgtt attaaagacc agttgaatat
     6661 tggatttcag gctatcaccg ccggttagct ccgcaacacg cgccagcagg aacggtgtac
     6721 tctctttccc aataacaccc tgttcttcgg cttcagcaac ggcttgatca attgctgcat
     6781 tgatggtttc ttctggcatc gcaaactgtt ccgggatcgg gttcgctacc accaggccgc
     6841 cgttcaaacc ggtttgccac ttcactgcca tcgcgcgagc aatttcgcgg gcactgtcga
     6901 gacgaatgct gacttcaaat gggctggttc ggcagaaaaa cgcaggcagc gcttttgtct
     6961 gataaccaat taacggtaca ccgaaggttt ctaaatattc tgttgttaat cctaaatcga
     7021 gaatagattt tgcccccgca caaacaacag tgacattggt atttgccagt tcctgtaaat
     7081 cggcagaaat atcgaaggta tgttccgctc cgcgatgcac accaccaata cctccggttg
     7141 caaaaacttt aatcccagcc agtgctgcaa taatcatggt tgatgcaacg gtagtcgcgc
     7201 catttttccc tgcagcaacg acaaaaggta aatcacgacg actaactttc gtcacgttat
     7261 gtccttcacg gcccagcaat tcgatttctt ctttacttaa gcccactttc atcacaccgc
     7321 caataatggc gatagtggct ggtactgcgc cctgtttacg aattgtttct tctacttcaa
     7381 ttgcggtttg ggcattttgt gggaatggca ttccgtgaga aattatggtc gattccagcg
     7441 caacaaccgg ttttttattt tttaaagcat cctgtacttc agcggatatt tgtaataatt
     7501 caggggacag agttaattca gacattctgt gttctccact aacgatgtaa cattggcaat
     7561 tgataattca gggttgttgg tatattcaca ggcaagtgcc attgacgaac atccctgcgc
     7621 aaatcgaaca gaatcgataa acggcatacc gtctacccaa caggaggcca gccccgccat
     7681 catggcgtcg cctgccccag taacattgat gacattagtt ttaatagggg ccgaccaccc
     7741 actttcaccg ttgatatcac tgtagtaaac gccatcccct cccatgctca gcaccaggcg
     7801 gttcaggccg tgttgatgaa accaggcggc gacctttgcg acatcttcac gtccggaaag
     7861 tgcaattccg ctcaacgttt cggcttcaag acggttaggt tttaacgtat ggattttgct
     7921 cagatgatcg cggattttga cgcatttcca tgccgatacc ggatcaataa atacaggcgt
     7981 ttccccgcta ttttccagaa cccagaccag cgcctcttca ctaaggttgc aatccgcgac
     8041 aataaccttt gcaccgcgaa taaactcctg atgctgggca agaaattctg cagaaataca
     8101 gtcactaata ctcatatcat ttatcgcgac aagcatttct cctgtattat cgagcagtga
     8161 taaatagctg gatgtattct cacccggaac aattaagcat ttatcgacat aaacaccgga
     8221 ttgattagtt tgcgccagta atgattgccc ataaaaatca ccacctactg cacttaaaag
     8281 ccaggcattt ttccccagta acgccagatt atgagcgata ttccttccca cgccacctgg
     8341 ggtaaattta atttttccgg gattcgaatc cgcataattc aatggatgat gtgaataccc
     8401 agccacatca atattcgccg agccaataat tactatgtag tccttttcgc gcatagcacg
     8461 ccccctggca ataaattgcc aacgaagaaa ttgggtaaaa tagtcattac agtcaataag
     8521 actattagag cacgtgttca gatttaaaca catgttcata ataagcatgt ttaacgtaat
     8581 gtaaatcgat cagaaaaaac gaaatcggcc agatatgaag gcggtcacaa aatttgtcta
     8641 tttatgcagc aacaaaagag aaagttgtga tgtgtaacac acaatatggc aggcaagaaa
     8701 aagggcagag atgtctctgc cctcagattg ttgacaacgc gcgctttgtt catgctggat
     8761 gtggcgtaaa cgccttatcc gacctacaaa agcgcaaaaa ttcaatatat tgcagggttc
     8821 atgtaggtct gataagcata gcgcatcagg caattttgcg tttgtcatca tcggggcaga
     8881 gatgtctcag ccccgatgat gaagagaaat tatgcgtttt tttctactat ttgtgtttcc
     8941 gggcgtttga ggaaggcata ggccaaaccc gccaccagcg taccagcaat aattgctacc
     9001 aggtaaccca ataccggcgt aatagcgcca gggatcagca gaacaaacag accaccgtgt
     9061 ggtgccatca gtttcgcacc aatcgccatt gagattgcgc cagtcagcgc cccaccaacg
     9121 atacagcacg gtagcacacg catcggatca cgagcagcaa acggaattgc accttccgaa
     9181 ataaagcaca gaccaagtac cagagcggct ttgccacctt cctgctgcgc tttgtcgaat
     9241 ttgcgacgcg ccaccattgt tgccagaccc attgccagcg gtggcaccat acctgccgcc
     9301 ataatcgccg ccatcgggcc ataggtttga gtactcagca gacccacacc gaatgcgtac
     9361 gctgctttgt ttaccggacc gcccatgtca gtacacatca tgccaccgag gatcgccccc
     9421 agcagaaccg cattcgcagt ccccatggtc tgcagccagt gagtcaaccc atcgagaatg
     9481 ccagcaaccg gtttaccgat taggtagatc atcgccagac cgaccaccag actggaaatt
     9541 agcggaatga tcaggatcgg tttcagtgcc tccatactct gtggcagttt cagttgcgta
     9601 ctgattaact tcgcaatgta acccgccagg aagcccgcaa taataccacc aatgaagcca
     9661 gaaccggtgc taaccgccag cataccgcca atcagacccg gcgtgagacc cggacgatcg
     9721 gcaatggaga aggcaatata acctgccagt accggcacca tcagcgcaaa ggctgaacca
     9781 ccaccaatct gcatcagcgc cgctgccaac gtacccggct ctttaaacgc ttcgatacca
     9841 aaagcaaaag aaagcgcgat acacagacca cctgcaacga ccatcggcag catgtaagag
     9901 acgcctgtca gcaagtgacg gtatgcgcct gcactctctt tcttaccttc agtggtcgcc
     9961 gtttgagctt tgcccgccgg ttcatacggc gttgcttcag caaccgcttt atccagttcc
    10021 tgagcggttt tcttcagcgc cagaccggta gaggtgcggt acatcggttt accagcaaat
    10081 ttcgccagat ccacttcgat atctgccgcc acaatcacca gatccgctgc tgcgacttct
    10141 tcgggagtga ttgcattacc cgcgccaaca gaaccacggg tttcaacttt cacccaccag
    10201 ccacgttttt tcgcttcggt ttcaatggct tcagccgcca taaaggtgtg tgctacgcca
    10261 gtcgggcaag cagtcaccgc aactacgcgt ttcggaccgc tggcggcaac cggtgctgtc
    10321 gcagcgaccg gcgcagtgta aggtttcgca tggcctttgg cttcactcag gaacaactca
    10381 gggtgcgcaa ctgcccggga aatatcgccc agccagacat ttttaccgtt cagcgcgctg
    10441 tcattcggga tggaatcacc gagaacaatc gccatttctg cgtcgttcgg attgtcgatg
    10501 atttccagtt ttgcttttcg cgccgccgcg cccagcaggg tcttcgccat ataggcgcgt
    10561 gcctgaccga gattagcgtc aataatcagc agcgttttca ttatgcctct cctgctgtca
    10621 gttaaaaggt tgtaagtcga cgcgcgccat cattgcggcc aactgcggac gatcggtaat
    10681 acccacattg ctttgactta ccgccagggc tgcaacagct gtcgccagac gcagtgtgtg
    10741 ttcactggat tcacgcatca gcaagccata aatcaggcca ccaaccatag aatcccctgc
    10801 gccaacggtg cttacgacat cgactgacgg tggtttggcg atccattcgc cggaggcatt
    10861 aacccaaagc gcgccttcgg cacccagtga aataacaaca tgcgcaatac cttgttcacg
    10921 tagcgcatgt gcagcttcaa tcacatcttt catttcaggc agtttacggc ctgcccagat
    10981 ttccaactcg cggcggttag gtttcaccag ccacggtgcc gctttcaaac ctgctactaa
    11041 cgcttcacgg ctactatcaa agataatgca aggacactga ctacgcaggc gagtcatcca
    11101 gtcggtgaac gcttccgggc tgacgcctga cggtaagctt ccgctgacac agaccatatc
    11161 gaactgaccg agccagctca gagaatcagt cacaaagcgt tcccagtcgg cgggggtgac
    11221 ttcaaaaccc gagaagttga agtcggtcac ttcgccgtct ttttccgtca gcttgacgtt
    11281 aattcgggtg cgcccctgta caacctggaa acggttggca atgcccagct cgctgaacag
    11341 ttgctgaaaa ccatcctgat tgtctttacc caggaagccg ccaacggtga catcaattcc
    11401 caggtctttt aatactttgg ccacgttgat gcctttaccc gccgcatgca gaccggtggt
    11461 tttcaccagg ttcacttcgc cgcgttcaat ttccgggcag aaaccaacaa ggtcataagc
    11521 cggattaagg gtgatcgtag caacacgtct gctcattatg cgccctcccc aagaccagca
    11581 gcgatagcgt cgccaatggc tttcagcgcc tgttcagcat ctgcaccctg ggcggtaaag
    11641 cgtaggcgat gacctttctt aacgccaagt gccacaactt tcatcagact acgtccgttt
    11701 gccggtttgc cggtgccatc aaggtttgtc acggtaatat cactgttaaa ttgtttaatg
    11761 gtattgacca gcatggtacc tggacgagca tgcaggccgt gttcattgcg caccacaaac
    11821 tccgcgctta acacgtcgtc ggtcggcgca tcatcgctgg tcagcagcgc cagcaacgtt
    11881 gccgcatccg ctttcagcaa gcggtcagct ttattatcga gcaacaaatc agcgagacgc
    11941 ttaagaaccg cgatgggctg atcgtcattc atcgccacac tcaccagcat ggctgccgtt
    12001 tcgccgtcca catcaaaagc atttgccgca cggcttaccg caatcgcgct acgcagattg
    12061 ccttcggcgc tatcgctcag ccagataccc tgcccgaggt tcagcggttg ttcattgatg
    12121 gctttggtga cgaaagtggc gtcaactgcc cccgcttctt tcagacgcgc agcgttcagc
    12181 gcctgaagag tcagcagatc gctggcgacg atatccagcg tcagcatttc gttgtcgagc
    12241 ttcagctgct cactctgctt ttcgcccatc agcaatgcgc gaagttcttc tgctgttgtt
    12301 gctgacttca gttgttcagc aacggaatca tcgctcagta cgtgggtcag ctggcgtagc
    12361 aggcccagat gttcatccga gctggcagca ataccgatcg ccacgtacgc tacctggccg
    12421 tcaccccagg tgacgccttc cgggaactga aatacctgaa cgccggtttt cagcacctga
    12481 tcgcgggtgt cggtagtgcc gtgtggaata gcaataccat tgccgaggaa cgttgaggtt
    12541 tgctgttcgc gcgccagcat gccattgacg tagccttcag ctacattacc ggcctgcacc
    12601 agcgccgcag cgacctggcg aatcgcctct tctttgtctc cggccttttc gcccggatgg
    12661 atgtcctgta cggataactg gaacatagtt ctcctctctt gctgaattga aacgattcag
    12721 cttctatgag aaaaaaagcg ccaacctggc ttagggttaa agacaagatc gcgctgaaac
    12781 gtttcaagaa agcataatac ttttgtttca gcacgcaagg aaagccgtaa agttatgagc
    12841 aaaagtttga tgtgctgcac aattttttgt catttttcct aattgctgat gggaaaatcc
    12901 gttatcagca atttcatttc agcagcgtca gcagccaatc agcaacagtg aaatggcatt
    12961 ttgattattc gtggcgcttt tgatttcgct gtctgtttat tttctgacaa gcagcgtaaa
    13021 ctccgcgtct tcctcttcca gtgatcgacc agcatgcata actcccccgc agtcaccagc
    13081 gcgaaatcgt ttgacctgac ctcgatggcg tttttaatcg ttgcctttct caccggtatt
    13141 gcgggcgctc tgcaaacccc gacactcagt atttttctta ctgatgaagt acatgcccgt
    13201 ccggcgatgg tgggattctt ctttaccggc agcgctgtca ttgggattct ggtgagtcag
    13261 tttctcgccg ggcgctctga taagcgcggc gatcgcaaat cgctgattgt cttttgctgc
    13321 ctgttaggcg tgctggcctg cacccttttt gcctggaatc gcaactactt tgttttgcta
    13381 ttcgttggcg tctttcttag cagctttggc tcgaccgcta acccgcaaat gtttgccctt
    13441 gcccgtgaac atgccgacaa aaccggacgt gaggcggtga tgttcagctc ttttttacgc
    13501 gctcaggttt cactggcatg ggtcattggc ccaccgctgg cttatgcctt agcgatgggt
    13561 ttcagcttta cggtaatgta tctgagcgca gcggtagcgt ttattgtttg cggcgtgatg
    13621 gtgtggctgt ttttaccgtc gatgcaaaaa gagcttccgc tggcgaccgg cacggttgaa
    13681 gcgccgcgcc gtaaccgtcg cgatacgttg ctgctgtttg tcatttgtac attgatgtgg
    13741 ggctcgaaca gcctgtacat catcaacatg ccgctattta ttatcaacga actgcatctt
    13801 cccgagaaac tggccggtgt gatgatgggg accgccgcag ggctggaaat cccgaccatg
    13861 ttgattgccg gatattttgc caaacgtctg ggtaagcgct tcttaatgcg cgttgctgcc
    13921 gtgggtggcg tctgttttta cgcaggaatg ctgatggcgc attcacctgt cattctgttg
    13981 ggcttgcagc tgctaaatgc tatttttatt ggcattctgg gcggcatcgg gatgctctat
    14041 tttcaggatt tgatgcccgg tcaggcaggt tcagccacca cgctctatac caacacgtcg
    14101 cgcgtgggct ggatcatcgc aggatcagtg gcgggcatcg tcgccgagat ctggaattat
    14161 cacgctgtgt tctggtttgc gatggtgatg attatcgcca ctctgttttg cttactgcga
    14221 attaaagatg tttaaggcgc ggtcaacatt tccagatcga taagccaggt cattgcctgc
    14281 tgacgcgagt taccgcacat ttcagcgctg gcttgtaagc ctgcacagac tttcggtcgc
    14341 agtggcgagg tgaaaatttt acagcgttgc tgttcatcaa gctgaatgca gggcgtattg
    14401 gcgggcttgc catctggcat accgggaata gggctggaaa ttgaaggggc agtgcaacac
    14461 gccccgcaac ccggacggca ttccatacgt tgttctctta aggttaacca ccggattgat
    14521 gagcgcgcac agtaccacct tttttgcacc agcaaaagtg cgaataccac ttgcccgaaa
    14581 ggcccgtcgc gagtactttg tcgcgatatt tttgacattt tcgactacag gaatttttcg
    14641 atgccaagag cgaacgaaat taaaaaaggt atggtactga attacaacgg caaactgctg
    14701 ctggttaagg acattgatat tcagtcgccc actgcccgcg gtgccgctac gctgtacaaa
    14761 atgcgttttt ctgatgtccg taccgggctg aaagtagaag agcgtttcaa aggtgatgat
    14821 atcgttgaca ccgtgacgct gacccgccgt tacgttgact tctcctatgt cgatggtaac
    14881 gaatatgtct ttatggataa agaagactat accccgtata ccttcaccaa agatcagatt
    14941 gaagaagagt tgctgtttat gcctgagggc ggcatgccgg acatgcaggt gctgacctgg
    15001 gacggtcaat tgctggcgct tgagctgccg cagaccgttg atctggaaat cgttgaaact
    15061 gcaccaggca tcaaaggggc atctgccagc gcccgtaaca aaccggcgac attgagcact
    15121 ggtctggtga ttcaggtacc agaatactta agcccgggcg aaaaaattcg tatccatatc
    15181 gaagaacgcc gttatatggg tcgtgctgac taacttcagc cgcatgcaga aaagggatag
    15241 ctcaggctgt ccctttttta atttattata ccaatcttct attttgcgct tcctgaacac
    15301 attaagcccg caattttcac tcgttgattg atcgccctca cacttcatcg cattaacaat
    15361 ccagaccaat ttcaattgtt gtcatataac tttacactgt cactgttaat taattgttac
    15421 taagacgtga ctcctatgaa tacaatcgcc tccgttacgc tcccgcatca tgtacacgct
    15481 ccacgctacg atcgacagca gttgcaatca cgtatcgttc attttggctt tggagccttt
    15541 caccgcgcac atcaggcatt actgaccgat cgtgtgctga atgcccaggg cggcgactgg
    15601 gggatctgtg aaatcagctt gttcagcggc gatcaactga tgagccaact ccgcgcacag
    15661 aaccatttat ataccgtgct ggagaaaggt gcggacggca atcaggcgat aattgtcggt
    15721 gccgttcacg aatgtctgaa tgcaaaactg gattccttag cggtaattat tgagaaattt
    15781 tgcgagccac aggtggcaat tgtttccctg acgattaccg aaaaaggcta ttgtattgac
    15841 ccggccaccg gtgcactcga caccagcaat ccgcgtatta ttcacgatct acaaaaccct
    15901 gaagaacctc actccgcgcc aggtattctc gtcgaagcac tgaaacgccg ccgtgagcgc
    15961 ggccttacac cgtttaccgt gctctcctgc gacaatattc ccaacaatgg ccatgtggtg
    16021 aaaaacgcgg tgctgggaat ggcagaaaaa cgttcgccag aactcgccgg gtggataaaa
    16081 gaacacgtca gttttccggg aaccatggtc gaccgcattg ttccggctgc aaccaacgaa
    16141 tcactggcgg aaatcagcca gcatctgggg gtgaatgatc cctgcgcgat tagctgcgaa
    16201 ccgtttatcc agtgggtggt tgaagataac ttcgtcgctg ggcgtcctgc ctgggaagtc
    16261 gcaggtgtac aaatggtgaa tgatgtcctg ccatgggaag agatgaaact gcgtatgctt
    16321 aatggcagcc actcttttct cgcttatctg ggttacctct caggattcgc ccatatcagt
    16381 gattgcatgc aggatcgcgc atttcgccat gccgccagaa cattaatgct ggatgagcaa
    16441 gcgccgacac tgcggattaa agatgtcgat ttaacacaat atgcggataa gttaattgca
    16501 cgttttgcta atccggcact gaaacataag acctggcaaa tcgcgatgga tggcagccaa
    16561 aaattaccgc aacgcatgct ggcaggtatt cgcatacatc tggggcgcga aacggactgg
    16621 tcgttgctgg cattaggcgt tgcaggttgg atgcgttacg tcagcggcgt tgatgatgcc
    16681 ggaaatgcca ttgatgttcg cgatccgctt agcgataaaa ttcgtgaact tgttgcggtc
    16741 agcagcagtg aacaacgcgt aaccgccctg ctttccctgc gtgaaatttt cggtgatgat
    16801 ctgccagata acccgcattt tgtgcaggcc atcgaacaag cctggcaaca aatcgcacaa
    16861 ttcggcgcgc atcaggcgct attaaacacc ctcaaaattt aacgatttct gcggttaaag
    16921 cggatgaagc tcgccttcgt ccgctctccc cttctctttt ctgccttttt tagccaggat
    16981 taacgctcag ttaacttacc agagtattgc ggagccgttg tgaccagaac caacctcatc
    17041 accggttttc tcggcagcgg gaaaactacg tcgattcttc atctgttagc ccataaagat
    17101 cccaacgaaa aatgggcggt actggttaat gaatttgggg aagtcggaat tgatggtgct
    17161 ttgctcgccg atagcggcgc actgttgaaa gagatccccg gcggctgcat gtgctgcgtt
    17221 aatggtttac ccatgcaggt agggttgaat accttactgc gtcagggaaa accagaccgc
    17281 ttgttgatag agccgaccgg gctgggccat ccgaaacaga tcctcgatct cttaaccgca
    17341 cccgtctatg aaccgtggat agatctgcgc gccacgttgt gcattctcga tccacgcctg
    17401 ctgctggacg aaaaaagcgc cagcaatgaa aacttccgtg accagctggc tgccgcagac
    17461 attattgtcg ccaataaatc cgaccgtgcg acgcccgaaa gtgagcaagc gctagagcgc
    17521 tggtggcagc aaaatggtgg cgatcggcaa ttaattcaca gcgagcatgg aaaaattgac
    17581 ggtcatcttc tggatttgcc gcgtcgcaat ttagccgagt tgcccgccag cgccgcgcat
    17641 tctcatcagc atagcgtgaa aaaagggtta gcagcgttaa gcctgccaga gcatcaacgc
    17701 tggcgtcgca gtctgaacag cggacaaggg catcaggcct gtggctggat attcgacgct
    17761 gatacggtat tcgacaccat tggtattctg gaatgggcgc gacttgcacc ggtggaacgc
    17821 gtcaaaggcg tgctgcgtat tcccgaaggg ctggtgcgaa tcaaccgtca gggcgatgac
    17881 ctgcacattg aaacgcaaaa cgttgcgcca ccggacagtc gtattgagct gatttccagc
    17941 agcgaagctg actggaatgc cttgcagagc gcgctgttga agcttcgttt agcgactacc
    18001 gcgtaaggtt gcctgcgttt ttcagaaaga taattagaga aaatatgatt aaaaatttgc
    18061 cgcaaatagt gttgttgaat attgtcggcc tcgcgctgtt tctttcctgg tatattccca
    18121 ttaatcatgg attctggttg ccgattgatg cggatatttt ttatttcttt aatcagaaac
    18181 tggtcgaaag taaggccttt ttatggctgg ttgcattgac caacaatcgc gctttcgacg
    18241 gttgttcact gctggcgatg ggaatgttga tgctgagttt ctggctgaaa gaaaacgccc
    18301 caggcagacg acgtatcgtg attattggtc tggtcatgct attaactgca gtggtattaa
    18361 accagctggg tcaggcacta attcctgtaa aacgggccag cccaacgttg acttttacca
    18421 atattaaccg cgtcagcgaa ctgctctctg ttcccacgaa agatgcctca cgagatagct
    18481 ttcccggcga tcacggtatg atgctgctta ttttttcggc attcatgtgg cgttatttcg
    18541 gcaaagttgc aggccttatc gcccttatta tttttgtggt ttttgcgttt cccagagtaa
    18601 tgattggcgc acactggttt actgacatca tcgtcggttc catgaccgtg atattgatcg
    18661 gcttgccctg ggtgttgctg acgccattaa gtgatcgatt aatcaccttt tttgacaaat
    18721 cactacctgg aaaaaacaaa catttccaaa acaaataact cacagtaatt aacatcatca
    18781 ggtttatttt tatagtgatg ataatcctga tgatgcgcct cctgctttca tccatcgagc
    18841 gcaaaaatca ttctctaagt aaatgaatgg attgcatgcg tttcactcaa tcgtacttta
    18901 attgaccaat cccgcttatt aactttctgt atcacttttt cttataaaaa atcatgtaaa
    18961 accgctcgcc aagaccgcac caatcgggta atctcgaact cgttttgcct cggcggtaga
    19021 ttatcctcac agcatataat tttgtgcgtt agtccacaga tttggcctta aggaattgtt
    19081 tcaacatgcc caggtaatta gtctcgtgtc gcttggcatt tttttataac gatatttgtc
    19141 gttaaggact tcaagggaaa acaaacaaca tggtcaaatc tcaaccgatt ttgagatata
    19201 tcttgcgcgg gatccccgcg attgcagtag cggttctgct ttctgcatgt agtgcaaata
    19261 acaccgcaaa gaatatgcat cctgagacac gtgcagtggg tagtgaaaca tcatcactgc
    19321 aagcttctca ggatgaattt gaaaacctgg ttcgtaatgt cgacgtaaaa tcgcgaatta
    19381 tggatcagta tgctgactgg aaaggcgtac gctatcgtct gggcggcagc actaaaaaag
    19441 gtatcgattg ttctggtttc gtacagcgta cattccgtga gcaatttggc ttagaacttc
    19501 cgcgttcgac ttatgaacag caggaaatgg gtaaatctgt ttcccgcagt aatttgcgca
    19561 cgggtgattt agttctgttc cgtgccggtt caacggggcg ccatgtcggt atttatatcg
    19621 gcaacaacca gtttgtccat gcttccacca gcagtggtgt tattatttcc agcatgaatg
    19681 aaccatactg gaagaagcgt tacaacgaag cacgccgggt tctcagccgc agctaataaa
    19741 ccgtttggat gcaatccctt ggctatcctg acgagttaac tgaaagcact gcttaggcag
    19801 tgcttttttg ttttcattca tcagagaaaa tgatgtttcc gcgtcttgat ccaggctata
    19861 gtccggtcat tgttatcttt taaatgttgt cgtaatttca ggaaattaac ggaatcatgt
    19921 ttatacgcgc tcccaattct ggacgtaagc tcctgcttac ctgcattgtt gcaggcgtga
    19981 tgattgcgat actggtgagc tgccttcagt ttttagtggc ctggcataag cacgaagcca
    20041 aatacgacac actgattacc gacgtacaaa agtatctcga tacctatttt gccgacctta
    20101 aatccactac tgaccggctc cagccgctga ccttagatac ctgccagcag gctaaccccg
    20161 aactgaccgc ccgcgcggcg tttagcatga atgttcgtac gtttgtgctg gtaaaagata
    20221 aaaaaacatt ctgttcatct gcgaccggtg agatggacat tccactcaat gaattgattc
    20281 cggcgctcga cattaataaa aacgtcgata tggcgatctt acccggcacg ccgatggtgc
    20341 cgaacaaacc cgcaatcgtc atctggtatc gcaacccttt gctgaaaaat agcggcgtgt
    20401 ttgccgctct gaatctcaac ctgacgcctt cactctttta tagttcacgg caggaagatt
    20461 acgatggcct cgccctcatt attggtaata ctgcgctatc taccttttct tcacgtttga
    20521 tgaacgttaa tgaattaacc gacatgccgg tccgtgaaac taaaattgcg ggcattcctt
    20581 tgaccgttcg gctttatgca gatgactgga catggaacga tgtgtggtac gcatttttac
    20641 tgggtggcat gagtggaact ttcgttggac ttctctgcta ttacctgatg agtgtgcgta
    20701 tgcgcccagg cagagaaatc atgaccgcca tcaagcgcga acaattttac gtggtgtatc
    20761 aaccggtggt tgatacacaa gctttgcggg taacgggcct ggaagtactg ctacgctggc
    20821 gacatccagt agcaggtgaa atccccccgg atgccttcat taactttgcc gaagcgcaaa
    20881 agatgattgt gccactgact cagcacctgt ttgagttgat tgcccgcgat gccgcagaat
    20941 tagaaaaagt actgccggta ggcgtcaaat ttggcattaa cattgcgcca gcccacttgc
    21001 acagcgaaag ctttaaagcg gatatccaga aactgctcac ttccctgccc gcacaccatt
    21061 tccagattgt gctggaaatt accgagcgcg atatgctgaa agagcgagaa gccacacaac
    21121 tcttcgcctg gctgcattcg gtcggcgtag aaattgctat tgatgacttc ggcaccgggc
    21181 acagcgcgct tatctatctt gagcgtttta cgctcgatta tctgaaaatt gatcgtggat
    21241 ttatcaacgc catcggtacg gaaacgatca cttcacccgt acttgacgcg gtgctgacgc
    21301 tggcgaaacg tctcaatatg ctgacagttg ctgaaggggt cgaaacgcca gaacaggcac
    21361 gatggctaag cgaacgcggc gttaatttca tgcaaggcta ctggattagt cgcccgttac
    21421 cgctggacga ttttgttcgc tggctgaaga aaccgtatac gccgcagtgg taaggtgtgc
    21481 ttacgtccct tattattcat agtgaaagca tgccggattg cggctaatga tgagtaaaag
    21541 gaaatccgtt gcagatgatt gtgcgcatac tgctgctgtt tatcgctctg ttcacctttg
    21601 gtgcgcaggc gcaggctatc aaggaaagct atgcctttgc cgtactgggc gaaccccggt
    21661 acgcatttaa tttcaaccat tttgattatg tgaaccccgc cgcgccaaaa ggtgggcaaa
    21721 taacgttgtc tgccctcggc accttcgata atttcaaccg ctatgcactg cgcggcaacc
    21781 cgggcgcacg caccgagcag ctgtacgaca cgctatttac gacttccgat gacgaaccag
    21841 gcagttatta cccgctgatt gctgaaagcg cacgctatgc tgacgattat tcctgggtgg
    21901 aggtcgctat taatccacgt gcccgttttc atgatggttc gcccattact gcccgcgatg
    21961 tagagtttac ttttcaaaaa tttatgaccg aaggcgtgcc gcaatttcgt ctggtctaca
    22021 aaggcaccac cgtcaaagcc attgcgccgt taaccgtgcg cattgagtta gctaaacccg
    22081 gcaaagaaga tatgctgagt ctgttttcgc tgccggtatt tccagaaaag tactggaaag
    22141 atcacaaact tagcgacccg ctcgccacgc ctccgcttgc cagtggtccg taccgcatta
    22201 cgtcctggaa aatggggcaa aatattgtct attcccgcgt gaaagattac tgggcagcaa
    22261 acttaccggt aaaccgtgga cgctggaatt tcgacaccat tcgctacgat tattacctcg
    22321 atgataatgt cgcctttgaa gcgtttaaag caggtgcctt tgatttgcgt atggaaaacg
    22381 acgccaaaaa ctgggccacg cgctataccg gtaaaaattt cgataaaaaa tacatcatca
    22441 aagatgagca aaagaacgaa tcagcccagg atacgcgctg gctggcgttt aatatccaac
    22501 gtccggtatt cagcgatcgc cgggtgcggg aagcgatcac tctcgccttt gactttgaat
    22561 ggatgaacaa agcgttgttt tacaatgcct ggagtcgcac gaacagttat tttcagaata
    22621 ccgaatatgc ggccagaaat taccccgacg ccgcggaact ggtgcttctg gcaccaatga
    22681 aaaaagatct accgccagaa gtcttcacgc aaatctacca gccgccggta tccaaaggcg
    22741 atggctacga tcgtgacaac ctgttaaaag ccgacaaact tctcaacgaa gcaggctggg
    22801 tgctgaaggg tcagcaacgc gttaatgtca caacgggtca gccactcagc tttgaattat
    22861 tgcttcccgc aagcagcaat agtcagtggg tattgccgtt ccagcacagc ctgcaacggc
    22921 tgggtatcaa catggacatt cgcaaggtgg ataactcgca aatcaccaac cgcatgcgca
    22981 gtcgcgacta tgacatgatg ccgcgcgtat ggcgggcgat gccgtggccc agttccgatt
    23041 tacagatttc ctggtcatcg gaatatatca attccactta taatgccccc ggcgtgcaaa
    23101 gcccggttat cgactcgctg atcaaccaaa ttattgccgc gcagggaaat aaagaaaaat
    23161 tactgccgtt ggggcgagca ctggatcgcg tattaacgtg gaattattac atgctgccaa
    23221 tgtggtacat ggcggaagac cgtctcgcct ggtgggataa attctcccaa cccgctgtac
    23281 gccctgttta cagcctgggt attgatacct ggtggtatga cgttaataaa gcgacgaaac
    23341 tgccgtcagc cagacaacag ggagagtaga tgggcgctta tctgattcgc cgtctgttgc
    23401 tggtggtccc aacgctatgg gcgattatca ctatcaactt tttcatcgtg caaattgcgc
    23461 ctggcggtcc ggtcgaccag gccatcgccg ccattgagtt tggcaatgcc ggagtattac
    23521 ccggcgcagg cggtgaaggt gttcgcacca gccatgcgca aacgggcgtc ggcaatatca
    23581 gcgacagtaa ttaccgtggc ggacgcggat tagatccaga agtgatcgcc gagatcactc
    23641 atcgctacgg tttcgataag ccgatccacg aacgttactt caaaatgctc tgggactaca
    23701 tccgttttga ttttggcgac agcctttttc gcagcgcctc ggtgctgacg ctgattaaag
    23761 acagtctgcc ggtctccatc accctcggat tgtggagcac gctgattatc tatctggtgt
    23821 cgattccgtt aggcattcgc aaagctgttt ataatgggag ccgttttgac gtctggagta
    23881 gtgcatttat catcatcggc tacgccattc cggccttttt gtttgccatc ctgctgattg
    23941 tcttcttcgc aggcggcagc tatttcgacc tgttccctct gcgcggcctg gtttccgcta
    24001 actttgattc gctgccgtgg tatcagaaaa tcaccgatta tctgtggcat atcacgctgc
    24061 cggtgctggc gacagtgatt ggcggctttg cggctctgac catgctgaca aaaaactcat
    24121 tccttgatga agtgcgtaag caatacgtgg tgacggcacg tgcgaaaggg gtaagtgaaa
    24181 aaaatattct ctggaaacat gtgttccgca acgccatgct gctggtgatt gccggttttc
    24241 cggcgacgtt tatcagcatg ttttttaccg gctcgctgct gattgaggtg atgttttcac
    24301 tcaatggtct tggcttactg ggctacgaag cgaccgtctc gcgcgattat cctgtaatgt
    24361 ttggtacctt gtatattttc accctgattg gcctgctgct gaatattgtc agtgatatca
    24421 gctatacgct ggtcgatccg cgtatagact ttgagggacg ttaatgtcgc gactcagccc
    24481 cgtcaatcag gcccgttggg cgcgttttcg ccataaccgt cgcggctact ggtcgttatg
    24541 gattttcctc gtcttgtttg gtttgagttt gtgttctgaa cttatcgcca acgataaacc
    24601 gttgctggtg cgttatgacg gcagttggta tttcccgttg ttgaaaaact acagcgaaag
    24661 cgattttgga ggcccgctgg caagtcaggc tgattatcag gacccgtggc tgaaacaacg
    24721 gctggaaaat aacggctggg tactgtgggc accgattcgc tttggtgcta ccagtatcaa
    24781 ctttgctacc gataagccct tcccttctcc accctcccgg caaaactggc tgggaacgga
    24841 tgccaatggc ggcgatgtgc tggcgcgcat tctctatggc acgcggatct cagttctgtt
    24901 tggcctgatg ctgaccctct gctccagcgt gatgggcgtg ctggcggggg cgctacaagg
    24961 ctattacggt ggtaaagtcg atctctgggg gcaacgtttt attgaagtat ggtcggggat
    25021 gccgacgctg tttttgatta ttttactttc cagcgtcgta cagcctaact tctggtggct
    25081 gttggcaatt accgtcttgt ttggctggat gagtctggtc ggtgtggtgc gggcggagtt
    25141 tttacgtacc cgtaatttcg actacatccg cgcggcgcag gcgcttggcg tcagcgatcg
    25201 cagtatcatc ctgcgtcata tgttgccaaa tgccatggtc gctaccctca cttttttacc
    25261 gtttatttta tgtagttcga taaccaccct gacctcactc gatttcctcg gcttcggtct
    25321 gccgctcggt tcaccgtcac tcggtgaact gctgttacaa gggaaaaata accttcaggc
    25381 cccgtggctt gggatcaccg ccttcttatc ggtggcgata ttgttgtctt tgctgatctt
    25441 tattggtgaa gccgtccgcg acgcatttga tcctaataag gcggtgtagc atgacgcaaa
    25501 ctctgttagc gattgaaaat ttgtcggtgg gttttcgcca tcagcaaacc gtacgtacag
    25561 tagtcaatga tgtttcacta cagattgcgg ctggcgaaac gctggcgctg gtaggtgagt
    25621 ctggttcagg taagagcgtt accgcgctgt caattttacg cctgctccct tgcccgccgg
    25681 ttgaatatct ctccggcgat attcgttttc atggcgaatc gctgcttcat gccagcgatc
    25741 aaacgttgcg cggtgtacgc ggtaataaga tcgccatgat ttttcaggag ccgatggtgt
    25801 cgttaaatcc attacatacc ctggaaaaac agctttatga agtactttca ctccaccgcg
    25861 ggatgcgtcg ggaagcggct cgtggcgaaa ttcttaactg ccttgatcgc gtcggtatcc
    25921 gccaggcggc aaaacggctg acagattatc cgcatcagct ctccggcggc gaacgccagc
    25981 gggtgatgat tgcgatggcg ctgttaacgc gaccggaatt attaattgcc gatgaaccaa
    26041 ccaccgcgct ggacgtctct gtccaggcgc agattttaca gctgttgcgc gaactgcaag
    26101 gcgagctgaa tatgggtatg ctgtttatta ctcataacct cagcattgtc agaaaactgg
    26161 cccaccgcgt ggcggtaatg caaaacggtc gctgtgtcga gaaaaataac gccgctacgc
    26221 tgtttgcctc acccactcat ccttacacac aaaagctact caacagtgaa ccatctggcg
    26281 acccggtgcc attgccagaa cctgcctcta cgttgctaga tgttgaacaa cttcaagttg
    26341 ccttccccat tcgtaaaggg atcttgaagc gcattgtgga tcataatgtg gtggtgaaaa
    26401 acatcagttt tacgctacgg gcgggtgaaa cactgggttt agtgggcgag tccggttccg
    26461 ggaaaagcac gacgggactg gcgctgctgc gactgattga ctctcagggc agcatcgtct
    26521 ttgacggtca gccactgcaa aatttaaatc gccgccagct gttacctatt cgtcatcgca
    26581 ttcaggtggt atttcaggat ccaaactcct cactcaaccc acgactcaac gttttgcaaa
    26641 ttattgagga aggcttacgg gttcaccagc cgacgctttc tgccgcacaa cgcgaacaac
    26701 aagtgatagc cgtgatgcat gaagtgggat tagatcctga aacacgccac cgttatccgg
    26761 cggagttctc tggtggtcag cggcaacgta ttgcgattgc cagggcgtta attcttaagc
    26821 cctcgctgat cattcttgat gaaccaacat catcactcga caaaacggtg caggcgcaaa
    26881 tattgacgct attgaaatca ttgcaacaaa agcatcaact ggcctatttg tttatcagcc
    26941 acgatttgca cgttgtccgc gcgttatgtc atcaggttat cgtactgcga caaggggaag
    27001 tagtggaaca aggaccgtgc gcgcgcgtgt ttgccgcacc gcagcaggag tatacgcgtc
    27061 agctactggc gttgagctga cgcttaaaaa ggattgtagt ttgaaaaggg ttcggcaatt
    27121 gccacaccaa aatttttcaa tcggcatgtt gcagcaaatt cgtcctggcg atttacaaac
    27181 aggcacggct caccttcaca ctccagcacc gaacatggaa cttcgatgtc gcttaacgcc
    27241 tggcttagtt tgtacatcac cgaccatgca ttatcaccat ccgggctaag caatttaagc
    27301 gccaccaggc tgttgtcacc gcacggtcca ttttgcggaa tcggttcaac cttcgaacct
    27361 gcgaaaccag cagaccagcg atagttctgc ggcagtcgat ggacgattga gagttgtaat
    27421 ggagtcactt attttccccg gaagcacatt tatttcacag tttgtttaca tcaactttaa
    27481 cacatcatca acaatccgtc tttatacaga tcgtaaagat tgatgcctcg ccagcgtggc
    27541 ctctatggct ctgatttaaa taatatctgc gggcgtggtc acgtttgcgg ggctatatgt
    27601 acccgtttct gcgatctaac tcaacctttt taactacaat gatgtgactt tttacataaa
    27661 ttgattttac ataaaatata catacatcgg gggaatgata gatttgtggt tgacatgtat
    27721 caacaaagga agccttttag cttcctcgtt gtgcaataga tcaccgtttt ttcggccgac
    27781 tggcgtacaa acagaagaga atggagctgg ttgcgcagaa tgcaattgac caaatcatcg
    27841 gccatgcaga gttaaaggtc gcaagagaaa gtaatgcgcc aacaattgcc cctatgccga
    27901 aacggaaggt tcctgccagc gaagatgccg ttcccgccat atggggaaac tcatcaagaa
    27961 tgaccgccat cgcattggat gacaccatcg acacgcagcc cacaaacgcc gcaacgccaa
    28021 ccaccagcga ccagaatccc agccccagca gtgcactgat gaccatccac gctgccataa
    28081 taaactgtat ccacaacccc gagcggaaca tatttaacgc gccaatacgg cggacgaagc
    28141 ggctgttaaa gatggtcatc acgaacagaa aaagaatgtt tagcgcaaag taataaccaa
    28201 agttttccgg cgcgacgtgg ttgatttcaa tgtagacaaa cggtccggcg cttaagaatg
    28261 agaacatccc ggcaaagctg aaaccgctgg ccagcatata actcaggaca cgcttatgac
    28321 ggaacagcgc cgcaaagtta ccaatagtgg tacgaatgtg aaaaggctgg cgacgctccg
    28381 gtggtaaggt ttctttgatc agaaagaaga tcattgccga agccagaatc gccgccacgg
    28441 cgaggatcca gaagatgtaa tgccagctca gccacaccag cacccagccg ccaactatcg
    28501 gtgccatcag cggtgcaatg gtcgtcacca gcatgacaaa cgacatcatc cgtgagaact
    28561 cttctttcgg gtagatatca cgcatcaaag cgttgataac cacgctggcc gcagccgcag
    28621 ccagcccgtg gaagaaacgc atcacaatca gctgatcgat ggtttgcgcc aacgcacacg
    28681 ccaccgcggc ggcggcaaac accagcgtac cgccaagaac caccggctta cgcccgaagc
    28741 tgtctgccat cggcccgtag attaactgcc ccaacgcaaa gcccagaata taagtactca
    28801 gggtcatctg cgtactgccc gccggtacac caaactgcgc tgaaattacc ggtagcgcag
    28861 gcagatacat atcaatcgac agcggcatca acatggccag caggccaagg ataaaaacaa
    28921 tagcaaacga cgaatgctgt cgggtggtca caacgggctc ctgaaagtca ttgaaaagtt
    28981 agacgacgct ggcaatttct tcttcagtta acggacgata ttcaccgggg gctaaatcag
    29041 catccagcgt aataccgcca atacgttcac gatgcagctc aaccacatgg ttacccacgg
    29101 cggcgaacat acgtttcacc tgatgataac gcccttcgct gatggtcaga cgaacctgcg
    29161 ttggggtaat cacttccagc accgccggct tagtgagatc tttttcgtta tgcagctgca
    29221 cgcctttagc aaattgctct gccgtatcgt cagctacggg tgattccagc gtcaccagat
    29281 aggtcttctc gcaatgatgg cgcggggaag taatgcggtg cgaccactga ccatcatctg
    29341 tcattagtac cagaccggtg gtatcgatat ccagacgccc tgcagcgtgc agtttccacg
    29401 ctaccggttc atcaagaaaa tagagcaccg ttgggtgatc cgggtcgtcc gtggagcaaa
    29461 catagccctg aggcttattg agcatgaagt aacgtggacc gtgttgctgc gccagcgggt
    29521 tgccatcgta agcgacatca tgttcaggga gcagtttgaa cgctgcatta cggacgattt
    29581 cgccatcgac ggtgacacga ttgccgcgga tttcacgccc ggcaatagca cggctaacgc
    29641 cgagttgctg tgcgataaat ttatcaagtc gcatgtgtgt gattttgcct gtaaaaaacg
    29701 gaggtcgggt aatgacccga aaatctgaac tgttgtctgc ccagtatagc ggtctaataa
    29761 cttccctcaa gggaaaaaga tccgtggcat actattagca gaataatcta cctacgcgag
    29821 accatgattt ttacactccg cccatatcag caagaagccg tggatgccac gctcaaccat
    29881 tttcgtcgtc ataaaacccc tgccgttatc gtgctgccca ccggtgcagg taaaagcctg
    29941 gtgatagcgg aactggcgcg gctggcgcgt ggtcgcgtgc tggtgctggc acacgttaaa
    30001 gaactggtgg cacaaaacca tgcaaaatat caggcgctgg ggctggaagc cgatattttt
    30061 gccgccgggc taaagcgcaa agagagccac ggtaaagtgg tatttggcag cgtgcagtcg
    30121 gtcgcccgta atcttgatgc ctttcagggt gaattttcgc tgttgattgt cgatgaatgc
    30181 caccgtattg gtgacgatga agagagccag tatcagcaaa tcctcactca cctgacaaaa
    30241 gtgaatcccc acttacgcct gctggggctg actgccacgc cttttcgact gggcaaaggc
    30301 tggatttatc agttccatta tcacggcatg gtacgcggcg atgagaaagc ccttttccgt
    30361 gactgcattt atgagctgcc gctgcgttat atgattaaac acggctatct gacgccgcca
    30421 gaacggctgg atatgccagt agtgcaatac gatttcagcc gcttgcaggc acagagtaac
    30481 ggactgttca gcgaagccga tctcaaccgc gagctgaaaa aacagcaacg cattacgcca
    30541 cacatcatca gccaaatcat ggagtttgct gcaacgcgca aaggggtgat gattttcgcc
    30601 gcgacggtcg aacacgcaaa agagattgtg ggattactgc ctgccgaaga tgcagcactg
    30661 attactggcg acacacccgg cgctgagcgc gatgtgttaa ttgaagattt taaagcccag
    30721 cgttttcgct atctggtcaa cgtcgcggta ctgaccaccg gatttgacgc cccgcacgtc
    30781 gatcttatcg ccattctgcg ccctaccgaa tcggtgagtc tttaccaaca aattgtcggg
    30841 cgcggcctgc gcctggcccc cggcaagact gattgcttaa ttcttgatta tgcgggtaac
    30901 cctcacgatc tctacgcgcc ggaagttggt acaccgaaag gcaaaagtga caacgttccg
    30961 gtacaggttt tctgccctgc ctgcggtttt gccaacacct tttgggggaa aacgaccgcc
    31021 gacgggacat tgattgaaca ctttggtcgt cgctgtcagg gatggtttga agatgacgac
    31081 ggacatcgcg aacagtgtga cttccgtttc cgttttaaaa attgcccgca atgtaacgct
    31141 gaaaatgata ttgccgcccg ccgttgccgc gagtgtgaca ccattctggt tgacccggat
    31201 gatatgttaa aagcggcgct acgactgaaa gacgcgctgg tattacgctg tagcggcatg
    31261 tctttgcagc atgggcacga cgaaaaaggc gaatggttga aaatcaccta ttacgatgaa
    31321 gacggcgcgg atgtgagtga gcgtttccgt ctgcaaacgc ccgcccagcg aactgccttc
    31381 gagcagcttt ttatccgccc gcatacgcgc acaccgggca tcccgctgcg ctggatcacc
    31441 gccgccgata tcctcgccca gcaagcctta ttgcgacacc cggattttgt cgtcgcccgc
    31501 atgaaaggcc agtactggca agtgcgtgaa aaagtgttcg attacgaagg tcgttttcgt
    31561 cgggcgcacg aattacgcgg ttaatggtag ttttgattga tgtataaggc gattgagtat
    31621 agaatctcgc ccgcttttgc atacgcaaag cagatcactt acctgttgct gggtcgcctg
    31681 tagcaggatt aatttaagag agaaagaaat gtttactatc aacgcagaag tacgtaaaga
    31741 gcagggtaag ggtgcgagcc gccgcctgcg tgccgctaac aagttcccgg caatcatcta
    31801 cggtggcaaa gaagcgccgc tggctgtcga actggatcac gacaaagtca tgaacatgca
    31861 agttaaagct gaattctaca gcgaagttct gaccatcgtt gttgacggta aagaaatcaa
    31921 agttaaagct caggacgtac agcgtcaccc gtacaaaccg aagctgctgc acatcgactt
    31981 cgttcgcgct taattgctga ataagttgta aaaaaccccg ctccggcggg gttttttgtc
    32041 tctgcaaatt atgcctgatt acggtattgc tattttttgc aggccagata aggcgttcac
    32101 gccgcatctg gcattcagaa cacaaaaccg attaattgcc gccagacgtc cggcgctgca
    32161 attgatcgcg caagttcggc ggcgtgcctt taatggtcag ggtatcggtt gctggatccc
    32221 agaagatgcg ttcacctaac agcatcgcat caaagttaat agtcaagcca ccgccgctac
    32281 cggcaaactt cgtcaactga cgcagtgtgc tacggtctgc cgggaagctc tcttccagtt
    32341 cgtagccttt ttcagccgca aactccgtga agctcacttc gctgacgcct gccagctctt
    32401 tcgacagcga ttccagttca atctcttcac ctgcctgcag ctgctcatta cagtagctat
    32461 aaacctgctg gcgcacgttc tgtcgctctg ctttatccag ttgtgcttcg gcagtgaaat
    32521 catcgaccgc ttgcaacagc ccacggtttt gcgctttcgc gtttaaccct tcgctggcac
    32581 cgaggaaatc cataaagaaa tccgccactt tgcgccctac tcgccctttc aggaaagtga
    32641 gatagcgggt cgactctgga ttggtttccc actcggttaa atcgatacgc gcaacaatat
    32701 ccgcatgatt gatatcaaga taatgggttg ggttgatgtc gagattttcg ttaacacgca
    32761 tactgctcag gttgctcaga actgccacca gcagatactc caccgccaga tagcgatagt
    32821 ggcaaaacag cacgaatcca ccgtcagcaa aaggatattt cgctaattcg tcacgcaggc
    32881 gaccggttgc cgcgcggcta aatgccagaa agtcctcttc gccctgacgc tgtaagcgca
    32941 gcgtctgcgc cagttcactc tcttcgctaa acaagccgta agctttattt ttggcgctat
    33001 agacccgatg cagttctgcc accatctcaa cgacggtttc cgtcggttcc agcaatgaat
    33061 cgcgcaacac cagctcaagg ttctgctcat cacgcttgat aagctggtgc agagcaatct
    33121 ggttgatatc cagactcatg ataaactctc ctttaagacc gggcggtatt caaccaccgc
    33181 cggttaagcg acgcaataaa agatggctgt cattttacag aaggcggcgt aacgagaatg
    33241 atttaacgca taaaaaagca gaaaaaaaac cgttgctacg gtaatatgtt gccctttcat
    33301 caactaactg atttcgattt atgccacaaa tttcccgcta cagtgatgaa caggttgaac
    33361 aattgctcgc agagctgctc aacatactgg aaaaacataa ggctccgacc gacctttccc
    33421 tgatggtgtt aggcaacatg gtcactaacc ttatcaacac cagcattgcc ccggcccaac
    33481 gccaggcaat tgccaactct tttgcccgcg ccttacagtc ctctatcaac gaagacaaag
    33541 cgcactaagg gaaacagata acaggttatg gtaactcatc gtcagcgcta ccgtgaaaaa
    33601 gtctcccaga tggtcagttg ggggcactgg tttgcactgt tcaatattct gctttcgctc
    33661 gtcattggca gccgttacct gtttatcgcc gactggccga caacgcttgc tggtcgcatt
    33721 tattcctacg taagcattat cggtcatttc agcttcctgg tgttcgccac ctacttgctg
    33781 atcctcttcc cgctaacctt tatcgtcggc tcccagaggc tgatgaggtt tttgtccgtc
    33841 attctggcaa cggcgggaat gacgctatta ctgatcgata gcgaagtctt tactcgtttc
    33901 catctccatc ttaatcccat cgtctggcag ctggttatca acccagacga aaatgagatg
    33961 gcgcgcgact ggcagctgat gttcatcagc gtgccggtta ttttattgct tgaactggtg
    34021 tttgcgacgt ggagctggca aaagctgcgc agcctgacgc gtcgtcggcg cttcgcgcgc
    34081 ccgctggccg cattcttatt tatcgccttt atcgcctcgc atgtggttta tatctgggcc
    34141 gatgccaact tctatcgccc gatcaccatg cagcgcgcta acctgccgct ttcgtacccg
    34201 atgacggcac gacgttttct tgagaagcat ggcctgcttg atgcgcagga gtatcaacgc
    34261 cgtcttattg agcaaggcaa tccagacgcc gtttccgttc agtatccgtt aagcgaactg
    34321 cgctatcgcg atatgggcac cgggcagaat gtgctgttga ttactgtcga tggcctgaac
    34381 tactcacgct tcgagaagca gatgcctgcg ctggcaggtt ttgctgagca aaatatttcg
    34441 ttcacgcgcc atatgagctc cggcaacact acagacaacg gcatctttgg cctgttttat
    34501 ggcatctcgc caagctatat ggacggcatt ttgtcgaccc gtacgcctgc ggcgttaatt
    34561 actgcgctta atcagcaagg ctatcagctg gggttattct cgtcagatgg ctttaccagc
    34621 ccgctgtatc ggcaggcatt gttgtcagat ttctcgatgc cgagtgtacg cacccaatcc
    34681 gacgaacaga ccgccacgca gtggatcaac tggctgggcc gctacgcaca agaagataac
    34741 cgctggttct cgtgggtctc tttcaatggc acaaacattg acgacagcaa tcagcaggca
    34801 tttgcacgga aatatagccg ggcggcaggc aatgtcgatg accagatcaa ccgcgtgctc
    34861 aatgcactgc gtgattctgg caaactggac aatacggtgg tgattatcac tgccgggcgg
    34921 ggtattccgc tgagcgaaga ggaagaaacc tttgactggt cccacggtca tctgcaggtg
    34981 ccattagtga ttcactggcc aggcacgccg gcgcagcgta ttaatgcgct gactgatcat
    35041 accgatctga tgacgacgct gatgcaacgc ctgctacatg tcagcacacc tgccagcgaa
    35101 tattcgcaag gtcaggattt gttcaaccct caacgccgcc attactgggt taccgccgcg
    35161 gataacgata cgctggcaat taccaccccg aaaaagacac tggtgctgaa caataacggt
    35221 aaataccgca cttacaactt acgtggtgaa agagtgaaag atgaaaaacc acagttaagt
    35281 ttgttattgc aagtactgac agacgagaag cgttttatcg ctaactgatt aattataaat
    35341 cagttagcaa aatatcttac ttgcaatcgg tgtggaaaac ggtagtatta gcagccacga
    35401 gtcggcacgt agcgcagcct ggtagcgcac cgtcatgggg tgtcgggggt cggaggttca
    35461 aatcctctcg tgccgaccaa aaatcccaag aaaaccaacc tattgcggtt ggttttttta
    35521 tatctgcatt taattcgata aacagaccgc gacacatcac ggcctgttta ttttctgtta
    35581 tcagaacgtc cagactacac ccgcctgagt tgtgctgctt tcttcacagg agaaacgact
    35641 atcgataccg agcgtcaggc ccgtcaatgt ttgctcaaag ccagctcccg catcagtgtt
    35701 cacgctgttg cggctgttaa ttgccgaact ccacatcgcc gtatcgtaat taacgccttt
    35761 tacgctacca agacgcccac gcacggtgtc cagttctgca tcaaatacca gcggttgtgc
    35821 tgccgccata ttcagcacat ccgtggttga agggggcatt tgtacgcgat tctctgcgag
    35881 actccagcta tggttgccat tatccagcaa ggtatattca tacgtactga tatcaacaac
    35941 gcttccggca ttgcccaacg taaatgccgc atcaccgcgc cctcctttaa tcgtgggatc
    36001 attcgccgcg ccataaacct gtagcactcc attattgatc gtagtctgat ttaccgcgcc
    36061 acccatttga acaatttgat aaccatcatt taatactgcg tattctgcgg tgccatttat
    36121 ttcaacgtac tgatatcccc cgttaatttc agtattacta cttacaccaa attcttatat
    36181 atgctgttct ccgccaggat tgattttcgt attattggcg gccccataaa ccagctggat
    36241 atctttttcc aggacaacac catcaactgt ctcaccatac tcaacagttg atgctaatgc
    36301 tatgggtgaa tataacgctg tagccaccag aacagatatt gcggaacgac aaagagaaac
    36361 agaaccagat tggtgcatta agctttcatc ctatgaaatt aattgctgtt aaaagcattg
    36421 gggacagaaa atacccatag ctccataccc ggagtcagtt ttaaaaaact gtttaaagaa
    36481 atgcacaagt attgtgattg attttttagt tgtttttcct gatgagaagc tgatgcaaaa
    36541 ttccgtcttt ataatgaaga tgatgccaaa gcgaacgaca aagttgtagt tttcactaca
    36601 tgtccataca taaaatgggg taacattcac gcgcctggta gcgttaccaa cgctacgctc
    36661 aaacataatg attctaataa aacctcagga gactactatg cctgaagcaa cgccttttca
    36721 ggtgatgatt gtggatgatc atccacttat gcgacgcggt gttcgtcagt tactggagct
    36781 tgattctggc tttgaagtgg tcgccgaagc gggcgacggc gcaagcgcga tcgatctggc
    36841 gaatagactg gatatcgacg taatcttgct ggatctcaat atgaaaggca tgagtggcct
    36901 ggataccctc aatgccttgc gcagggatgg cgttaccgcg caaattatta tcctgaccgt
    36961 atcagatgcc tccagcgatg tctttgcact gatagacgca ggcgcagacg gttatctgtt
    37021 gaaagacagc gacccggaag tgttactgga agcaattcgt gccggagcga aaggcagcaa
    37081 agtctttagc gaacgcgtca atcagtactt acgtgaacgt gaaatgtttg gcgcggaaga
    37141 agaccccttc agcgtgctga cagaacgcga gctggatgtt ctgcacgagc tggcacaggg
    37201 gctgtcaaat aaacagattg cctcggtgtt gaatatttcc gagcagacag taaaagtcca
    37261 tattcgcaat ctgctgcgta aactcaacgt ccgctcacgc gtggcggcca ccattctgtt
    37321 cctgcaacaa cgcggggcac aataaaaata gcccgatggc atcaccatcg ggccactttt
    37381 tatttactct cctgcggcga caaatgttgc atcgcctgcg cgatactacg ttcgatcacc
    37441 gcacggcgag tatcgttggc aggtaagagt ttcaacatca tttcccacgc ggcaacggct
    37501 tcgccaaatc gttgctgctc aaaggcatta aatgcataca tgcttaacac acggatattg
    37561 ctatggtccg ttctcaccaa ctggcgcagc agttcgccgc cgaggcggtt gtcgttggga
    37621 tcagatgaac gtgtcaacgc ttcagcgtat cccagtgcag catcgctgtt tttcggatcg
    37681 aggcgatacg cagtggcgta tgcatcggtg gcgatactgg cgttacccag cgccatgcca
    37741 acgcggccca acataatcca gccttctata tctcccggat ttttttgcag ttgagtacgc
    37801 atccccagcg caaggcgcga catctcttct tcgttgagcg gatcggcttt cggatccagc
    37861 gccctgtcca gtaacgccgg agcctgtgcc gtggcctgct gccagatttt cacctgctga
    37921 taattgccag tctggtagta gctgacgcca gccacaatta acgccaccac aatacccggc
    37981 agataaacaa catatccggc acgcttacct tccggcacgc tttgttctgg aaacgcttcc
    38041 ggcaccacgc gtacccgacg ccgcgaacgg gcgtatatga cccaaacgcc aatgccaata
    38101 gccactactg gcagcaccca cagcagcacg gtcagcggcg ttaacggcgg atcgtaagtg
    38161 acgaagttgc cgtaacgcgc caccatataa tcgacaatct ctttcttact tttgccttcc
    38221 tgcatcagtt catacacttt ctggcgcagg tcggtggcaa tcatcgagtt ggaatcggca
    38281 atgctgttgt tctggcattt cgggcagcgc agttcttcag tgagctgacg gaactgttgt
    38341 tcctgtgctt catctttaaa ctgcaacacg tcgatggtcg ccagcgctga gccggagatc
    38401 atcagcatca gcacgcccaa taaaaacctc attgtgcggc ctccttactg tatttctccc
    38461 acagtggctt gatctcttct tcccagacgc gaggattgag atcgcccgca tggcgatagc
    38521 gaatgatgcc gttgccgtca ataaggaacg tttctggcgc gccatagaca ccgagatcca
    38581 gccctaacat gccgtcgcca tcaaacaggc ttagcgcgta aggattgccc agctctttca
    38641 gccagctgat tgccttctgg cgatcgtctt tatagttcat gccgaccacg cggatgccct
    38701 gcgcagaaag ctgattcaga tattgatgtt ccgcacggca ggtcggacac caggtcgccc
    38761 agacgttaag cagcaccggt ttgccctgag tcagcacatc cgcctgataa aactgccccg
    38821 gattgtccag tgattcgagg cgaaacttcg gcactggctt gccaatgagc gccgattcca
    38881 gattggtcgg atcatcccct tcggcattac gcgccagctg ccacagcagc gccgcggcaa
    38941 tcgccaggaa gataatcaac ggaattaaca ataccttgcg cttcatgcag cctccggcgc
    39001 agttttctgc gggttcacgc gcttacgata gcgaggatca aacagacaca gcagtccgcc
    39061 caacgccatc atcagcccgc ccgcccaaat ccagcgaaca aatggtttgt agtaaagacg
    39121 cacggcccac gcgccgtttt ccagctcttc accgagtgcc gcgtacaggt cacgcgtgat
    39181 gccaccgtca atcgccgctt cggtcatcat cgacccggca gtgttgtaat aacgtttttc
    39241 tgcatacagc actgtttccg gcttgccatc tcgcgttacg ccgatagtcg ccacaccgcc
    39301 acgccagttc ggaccggtca cctctttgac atcacggaag gtgaagcgat attcatgaat
    39361 atcgacgcta tcgccggact tcatgcgcac atcacgctca acgctatagt tctgactaaa
    39421 agcaatgcca acaattgtca ctgccagccc caggtgagcc gccaccatcc cccaataact
    39481 gaaggtggtt ttcgtgccgc gtgaaatacg cagcgcagct tccgcaattg ccagcaccgc
    39541 aatccagcag gccattgcca ggccgagcac cgtcatcgcc acaactttgc tttcgaacag
    39601 ccacggcaac agcagcgaca gcaccagcgt agagatgaag gcgataatca acaaattgcg
    39661 gattttacgt gggcgatcgc gtccccagcg caccagaggg ccgacaccaa gcagtagcgc
    39721 aaacggcacc atcagccagg taaacatggt gttgaagaac ggttcgccaa tcgaaatact
    39781 gcccagtccc agttgcttgt gcaccagcgg cagcaacgtc cccagcaaca ccaccagcat
    39841 cgcggcgacc agcaaaacgt tgttcgctaa cagcaaagat tcccgcgacc acagcgcatt
    39901 gtttacgcgt gagcgaactt tgtgtccacg cgcggcaaac agcagcagcg aaccgccaat
    39961 caccagcacc ataaaggcga ggataaacat accgcgcgcc ggatcagacg caaacgcgtg
    40021 taccgatacc agcacgccgg aacgcaccag gaaagtcccc agcagacaca acgagaaggc
    40081 actgattgcc agcagcaatg tccacgcttt gaagctggcg cgttgttcag tgaccgccag
    40141 tgagtgcatc agcgcagtcc ccaccagcca cggcataaac gaggcgtttt ctaccggatc
    40201 ccagaaccac cagccgcccc agccgagttc gtaataggcc catgcggaac cgagcacaat
    40261 gccgagcgtc aggaaaatcc acgccgccag cgtccacggg cgagtaaaac gcgcataagt
    40321 gctgtccaga cgcccactca gcaaagaagc aatggcaaaa gcaaacgcca ccgagaaacc
    40381 cacgtacccc atataaagca gaggcggatg gaagatcagc cccggatcct gcaacagtgg
    40441 gttaagatca cgcccttcaa tcgggaaatt cggcagcgtg cgtgagaacg ggttagaggt
    40501 aaagagaatg aacagcaaaa agccgacact gaccatcccc attatcgcca gtacgcgggc
    40561 aacaatatcc agcggaatac gctgactaaa aatcgctacc gcaaaggtcc agccgctcat
    40621 cagtagcacc cacagcagga gcgagccttc atgcgcgccc caggtagccg ccacgcgata
    40681 ccacaccgga agctgggtat tggagttgct ggcaacatag gtgacggtaa agtcgttgac
    40741 cacgaaagca ttgaccagca ccagaaatgc gccagccaca gacataaaca gcagccaggc
    40801 aaacaagcgg gaagacgcca tcatgcgcgc atctccgcgc gctacgcccc atagcggata
    40861 cacggacagc agcagcgcaa ttcctagcgc cagacacagc agcccgttac caatttccgg
    40921 catcatgatg ctgggtcctt ataaacactc gccgggcgac ggtggttagc ttccatcgct
    40981 ttctcaactt ctggtggcgt gtagttttcg tcgtgtttcg ccagcacttc tttcgcgagg
    41041 atatgattgc ctttttccag ttcgccctgc accacaacgc cctgcccttc acggaacaga
    41101 tccggcaaaa taccttcgta agagacatct actgaacctt cagcgtcgta aatggtgaaa
    41161 gttactttca gcgaattggg atcgcgctgc acactccccg gcatcaccat cccgccaacg
    41221 cgcagacgct gaccgacttc cggcatctgc tgagtttcac gcttgccgta gagaatctcc
    41281 cccggcgtat aaaagagatc aatattcgag cgcagcgcat atagcaccag accgatagtc
    41341 agcgccagcc ccgccagcac ggcacaggca atccacaagc ggtttttacg gcgaatattc
    41401 atgcagcctc ctgctgttgc gcagcacgta aacgcgcctc acgcgcccgc tgttgcgcca
    41461 cgccacgcag aattgcgcga tgttgcatca ccgagtacac gaccaaaacc accagcggaa
    41521 taacggtcat caccaccgcc agccagacaa aaaaggcgta accgcccatt gcgaaaaatt
    41581 cattccagga agcaaatgca ggggtcattt acggcctctt ttcagtatca gttcactcac
    41641 ccacggacgg cgtttttcca tcagcaaaat caaattacgc atccgcatca gcgtcagcgt
    41701 ggcagacagg agcaggaagc caaaaatcga ccagcgcagc ggcgaacgca tcgccggatc
    41761 gatactttgc tgcattcgcg ttgatccctg atgcagggtg ttccaccact ccacggagta
    41821 atgaataatc ggcagattca ccacgccaat cagcaccagg atacctgccg cacggccagc
    41881 cagacgacgg tcgtcgaagg cgtgccacag ggcaatgaca ccaacataca gaaacagcag
    41941 caccagttca gaagtcagac gtgcatccca tacccaccag gtgccccaca tcggttttcc
    42001 ccatgcagag ccggtaacca gggcaataaa ggtaaacacg gcaccaatgg gggccatcgc
    42061 cgccaccgcc aggttggcca ttttcatctg ccagaccagg ccaataaacg ctgccaccgc
    42121 cattgatgca taaatgccca tcgaccagat cgccgcaggc acatgcaggt agataatgcg
    42181 gtagctattt ccctgctgat aatcagccgg agcaaagccg aacccccaga tccagccgac
    42241 ggtaagcacg accacactgg caattgccag ccacggtata aaccagccac agatttgata
    42301 cagccgtggt gggatcgcca gttgatgcag tgttttccac atagtttcga taccagactc
    42361 gaacaaaaat cagtaatcca gcgttattga atgctgattc gtaacgctgc cgccgtcgca
    42421 aaaggactta atgtcgcggt gcctgccagc aacgcgccta aaattgccag atacccatca
    42481 acgggcaaat gcatagaagc cgcgtccatc gcggcggtgg caaagatgag taatgggata
    42541 gtcagcggta acaccagtat gctgagcagc acaccaccgc gcttaagtcc cactgtcagc
    42601 gccacgcccg gcgcaccgag aaagccaagc gtaggggttc ccagcagcag cgtcagcgcc
    42661 atcacttgcc agccataaac atccattccc agtagcattg ctaccagggg cgaaaggatg
    42721 agtaacggca gaccggttac catccagtgc gccatcacct tcgccagcac aacggcgggc
    42781 aagggtaacg gcaacaacat caattgttca agactgccgt cctgcaaatc gtcacggaac
    42841 agtcgctcga gtgccagcaa ggatgaaagc agcgcggcaa cccagataat gcccggtgca
    42901 atacgcgcca gcagttgcgg ctccggaccg atactgagcg gaaaaagggt aatgacaatc
    42961 aggaagaacc acagcgggtt ggcgatttcg gcgctatggc gaaacgctac gcgcagctca
    43021 agacggaaaa tgcgccagaa catcatgcgg cccccgtttg cgtcagtgaa atgcggcgaa
    43081 ttttactttc agcaacgttg agcggttggt gggtagtcag aatcacaatc cccccctgct
    43141 ccgtatgctg cgccatacgc tgggtcagac gatcgacgcc gttaacatca atcgcggtaa
    43201 aaggctcgtc gaggatccat aacgtggcac gggtcagcca cagacgcgct aaagcgacgc
    43261 ggcgttgttg cccggcagaa agttgattta ccggaatatc ttcgaatccg gcaagcccgg
    43321 cctgcgccag ggcttccaga cattgtgcgg tatcgccatc gcgatgataa aagtgcagat
    43381 tttctaacgc cgtcagccgg gttttgatcc ccggctgatg gcctatccat aacaggtttt
    43441 gatggtagct gtcgcgtacc tgatgcaagg gctgcccttg ccagagaacg tcacccgcat
    43501 cagggcgaga caaccccgtc agcaaacgga gaagcgttgt cttccccgcg ccgttgctac
    43561 cggtgatttg tacccactct cctgcgttca gcgtaaatga taagccacta aataaggttc
    43621 gttcatcccg ctcgcaaagt aactctctgg cttcaagcat acccacgcaa taaccctgtt
    43681 aaaaacctgg ctcgacttca cgcatgtcgg gcagcttgtg cgctatccct ttatggcaat
    43741 caatacaggt ttgcccatct ttcaccgcct ggtcatgcat cttcgcggca accgatttct
    43801 gggcggttgt atccatatac tcgaagttgt gacagttacg gcactcctgc gagttgttgt
    43861 ccttcatgcg ccgccactca ttctgtgcca tcgtcaggcg atgagcttca aatttctgcg
    43921 gcgtgtcaat aacgccaaaa attttaccat acagctcttt acttgctttg agcttgcgta
    43981 tcatctttgg cacaaactcg tgcggaacgt gacaatccgg acaggtcgca cggacgccgc
    44041 tacggttgtt gtagtgcacg gaatccatgt attcctgata caccgtgtta cgcatttcgt
    44101 ggcagctaat gcagaactct tcggtattgg ctttttccat cccggtgtta aagccgcccc
    44161 agaagacgat gccgccgaca aaaccgatca acagcagcgt ccctagcgcc agacggctgg
    44221 gggtacgcca ccatttccac aggcgcttaa tcagaccagg cttacggtca gaatttccca
    44281 taataacctc ttatttcccg taaccttttg atggggtaaa ggtattcccc acgattggcg
    44341 cggtatcggc ctgcggtacg tgacattgca gacagaaata acgacgcgga gccacttccg
    44401 cgcccacttt gccgtcgctg tccataaagt gagtaggact aatgcgcggc gcgccagtgg
    44461 tgcgataact ttcgacaccg tggcactgca agcagcggtt ggtattggtc gttacctgat
    44521 aaccttcaac gctatgcggg atcattggcg gctgattcac atagttcagc ggcatccgat
    44581 cctgctcttt tggcatccga atggcccctt cctgcgtccc ggatacttcc ggcgactggc
    44641 taaaatcgac gccattagcc gcccaaaccg cgccgcttac caccagggcc agcatcgccg
    44701 tccattgaca cagcgctttc ttcaggtcat ggcttttcat gatttcgctc ccgaactcca
    44761 tcgtgtagtt attgtaaata catcctcaga acagacatcc acgcagcgac cgcacgtcat
    44821 gcaatcgcgg ctggtgacct gcaccgggct ttgttcatcc agcaccgggg cacgtagcac
    44881 atgcggttcc gggcaaacat gaaaacaatc catacagcgg ttacatttct gccgatcggt
    44941 ggccgcaacg gtaatcacac ctttgctacc cagcacgcca tacagtgcac caaccggaca
    45001 aatgtgcccg caccagccgt gttcaacgac cagtagatca aataaaaaca gcgcgagaat
    45061 aagcagcgcg ccgctgccga agcccattac caggctacgc cccatcagag aaacggggtt
    45121 gatccattcc cagatgagcg tgccagttaa ggccgatccc accagaatga ccaccaacag
    45181 cacgtagcga atatggcgag ggatcgtcgc agactgattg aggtcaaatc gtctgcgtaa
    45241 ccaatttgct aagtcggtaa tcgggttcag cggacagacc cagctgcaaa ataatcgctt
    45301 tcctgccagg gcgtagagca cggtgataat caccgcaccg gtcaacgcca ccgtggcagg
    45361 cagatgaccg ctggcaagac tttgcagtgt catcagcgga tcggtcagtg gaacggtgtc
    45421 gaataacagg ctactgctgt agttgccgtg caagatccac acaccaaacc acggaccgct
    45481 caaaaacatc cccagcacga agaactgaca aaggcgacgt aacactaacc aacggtgact
    45541 gcgccaccag ccttttttct ccagcgcctc gcgcccggcg tcacgtttac gatttgccat
    45601 tgttcccctc cagccagccg aagcggtaat ggtgacctaa ctcccctttc gccagtgaca
    45661 gcggtaacac cttgattgcc ggttgttcca gcacgcacac tttttcgcac ttaccgcagc
    45721 cggtacaggc gtcgctgtga accgtcggca gaaagcgggc gtgcttaccg gttcgcgtgt
    45781 tgcgctccag ctccagggtg atggcctcat caattttcgg gcattcgcga taacaaacat
    45841 cgcagcgcag cccctgaaag ttgagacagt tttcctggtc caccagtacc gccagcccca
    45901 tccgcgcgtc gtcgatcgat tcgatctcac gatccagcgc accgctcggg cacactttgg
    45961 cgcacggaat gtcctcacac atttcgcaag gaatatcccg tgcgacaaaa tatggcgtgc
    46021 ccgccgacag accagaggcc agcgtcgcca gttttaaggt gtcgtaaggg caagcctgaa
    46081 cacactgacc gcaacgcaca caggcactgg caaaggcgtt ctcgtttatg gctccgggcg
    46141 ggcgcaaccg cacaccagat gcgcgtgcgg tttgctgttg taaccccagc gccacaccca
    46201 cggcagccag cccgcctgct gtgcgaacga catcgcgcag aaagcggcgg cgaccatttt
    46261 gaggtttcgc tgaccgggac ataatggctt acaccttctc cagtttgacc gcgcacttct
    46321 tgaagtccgt ctctttcgag agcggatcgg tcgcatccag cgtcagtttg ttaaccagct
    46381 gtgcggcgtc gaagaacggc atgtacacca ggccctgcgg cggacggtta cgaccgcgcg
    46441 tttcgacaat cgagatcact tcgccacggc gagaaaccac tttcactttg tcgccacggc
    46501 gcagatcgcg cgctttcgca tccagcgggt gaataaacag aaccgcttcc gggaaggcgc
    46561 ggtgcagttc cggtacacgg cgagtcatac tgccggtatg ccagtgctcc aggacgcgtc
    46621 cggtagagag ccacaggtcg tactcttcat ccggtgcttc cgccgcaggt tcgaacggca
    46681 gcgcgaagat caccgctttg ccatccggtt taccgtagaa cttgtagcct tcgcccgctt
    46741 tcacgtacgg gtcgttacct tcgctgtaac gccactgcgt ttctttaccg ttcaccaccg
    46801 gccagcgcag accgcgtgct ttgtggtagt catcgaacgg cgcgagatcg tgaccgtgac
    46861 cgcgaccaaa ccatgcgtac tcttcgaaca gccctttttg cagatagaag cccagctcgc
    46921 gggattcatc gttcagctga tcttccgcca gttcggatac cgggaatttg ctcacttccg
    46981 gagtggcata cagaacgtcg tacagcgttt tgccacgcag ttctggtttc ttcgccagca
    47041 gctcttccgg ccacacttct tcagttttga agcgacggga gaactgaact aactgccaga
    47101 gatccgattt tgcttcaccc ggtgcctgta cctgctgacg ccagaactga gtacggcgtt
    47161 cggcattacc gtaagcgccc tctttctcta cccacattgc ggtcggcagg atcaagtccg
    47221 ctgccagcgc actgaccgtc ggatacggat cggaaacgat gataaagttg cgcggatcgc
    47281 gccagcccgg catacgctct tcattaatgt tcggtccggc ctgcatgtta ttggtacaca
    47341 tggtccagta aacattcagt ttgccgtctt tcagcgcacg gtcttgtgcc accgcgtgca
    47401 gaccgatttt cgccggaatg gtgccactcg ggatattcca cttcttctcg caaatatcac
    47461 gatgtttctc gttagtcacc accatgtccg caggcagacg gtgagcaaag gtgcccactt
    47521 cacgcgcagt accacacgcg gaaggctgcc cggtcaggga gaacggaccg caacccggct
    47581 gggaaatttt gccggtcagc aggtgcaggt tgtagaccag gttgttggcc catacaccac
    47641 gggtatgctg gttgaagccc atcgtccagt aggagatgac tttcttgttc ggatcggcat
    47701 acagctgcgc cagttgttct aactggtctt tcggcacgcc ggtcatttcg gcggtttttt
    47761 ccagcgtata ttcagcaacg aaggctttgt aatcttcaaa gctcattggt tcggaggcgt
    47821 cagaacccgg attcttcgct gctttttcca gcggatgggt cggacgtaaa ccgtagccga
    47881 tgtccgtcgc tcctttgcgc aggttaacgt gtttgctgaa gaagtcctga tttatcgcat
    47941 tgttttgaat gatatagttg gcgatatagt tcaggatcac caggtcagat tgcggcgtaa
    48001 agatgatgcc gttatccgcc agctcgaagc tacgatgctg gtaggtagaa agcaccgcca
    48061 cggtgacgtt ctggttagaa agacgacggt tagtgatgcg cgaccagagg atcgggtgca
    48121 tctccgccat gttggagccc cacagcacaa acgcgtcagc ctgctcgatg tcgtcgtagc
    48181 agcccatcgg ctcatccata ccaaaggtac gcataaagcc aactactgcc gacgccatac
    48241 agtgacgcgc gttcgggtcg atgttgttcg aacggaagcc cgctttaaac agcttggacg
    48301 cggcataacc ttcccagata gtccactgac cagaaccgaa cataccgatc gattccggcc
    48361 ctttttcttt cagggcggtt ttgaatttct cttccatcac atcgaaggcc tgatcccagg
    48421 tgattggggt aaattcgcct tctttgtcat atttaccgtt tttcatacgc agcagcggct
    48481 gcgtcaaacg gtctttaccg tacatgattt tgggcaggaa atagccctta atgcagttca
    48541 ggccacggtt aaccggtgcg tccgggtcgc cctgacaggc caccacacgc ccctgctgcg
    48601 ttccgaccag aacgccgcaa cccgtaccgc agaaacggca cggcgcttta tcccatttaa
    48661 tggcttcctg ctgaccaaca acggcgcggg caacgcccgg cacgctgaga ccggcagccg
    48721 ccgcagcggc cgcaacggcg ttagctttca taaagctacg acgactgagt ttcatggtgt
    48781 ttcctcacct tgctcttcct gctggtgata aaccagcgac accgccagca cgccctctac
    48841 gttgcgtact gactcaatgg tttggatcag cgtttcgctg tcttctgctt ccaccaccac
    48901 aatcaactga ccgctcggcg cgtcgctgac agcaacttca cagccggtaa aggcgttcag
    48961 ttgggtgctg atgtctgaaa ttcgttcgct tttggcctgc acgaccaggc tgcaaacttg
    49021 ccagttagtg tgcatggaga tactccgcag ttatggctga taccggacag ctggctgcac
    49081 atgctccaca tccgttgcaa agttggctat taagttgcgg ctggtagatc ccggagagcg
    49141 tcgggcgaaa gataattgcc attggctcac agctatcctg acagcggcgg cattcaactg
    49201 actgatacgc caggcacgcg tccccaatgg tgaactgcaa atcccaggcc ctggtgtggc
    49261 gcggagaaaa tagcgattcg gggcaagcct gcgcgcaggc gtaacagaag ctgcactcgt
    49321 tatttttgaa attaacgctc ggatagccac ccgcgccgcg ttgcagaatg ttgttttcac
    49381 aagcattaat acaggcgtcg cagcgggtgc aatgggtcag aaaatgagat tcatcaccgg
    49441 accagggcgg acggataccg ttactggctt tgcgccagcg accagtgagt atgccccgac
    49501 gggatgcatc aatcttcaca ttgaccttcc atcattaacg cgctctgaaa attgagagcg
    49561 accaaataaa ccgcataatt aataagccat ttttatagcc gctaagatat taaaggatgt
    49621 gtcaaagatg cataccccga tcggggtaaa acctgtagca ggatcaaaag agtggcggga
    49681 agcgtggcaa aaacgggctt ttgctcacat ttcaaatggt tataaacata tttatatagc
    49741 gattaattcg ccagagatat ttctgctggt ttgttttctc atcagaattt aacactaaaa
    49801 gagcaggtaa aattatctga atgttcttta agttattcat aaagcaaatt aataaatctg
    49861 atgaatatgt taaccttcag cgacatcatc ggtgaaaacc tataaatgaa gaaggaaagc
    49921 aaaaaaatga agaccattct acctgcagta ttgtttgccg ctttcgccac cacttccgcc
    49981 tgggcggcag aaagcgtcca gccactggaa aaaatcgcac cttatccaca agctgaaaaa
    50041 ggcatgaagc gtcaggtgat tcagttaact ccgcaagaag atgaatctac cctgaaagta
    50101 gaactgttaa tcggtcagac gctggaagtc gactgcaatt tgcatcgtct cggcgggaag
    50161 ctggaaagca aaacgctgga aggctggggc tatgattatt atgtctttga taaagtgagc
    50221 tcgccggttt ccacgatgat ggcctgcccg gatggcaaga aagagaagaa atttgtcacc
    50281 gcgtatctgg gcgatgctgg aatgctgcgt tacaacagca agctgccgat cgtggtgtat
    50341 acgccagaca atgtggatgt gaagtaccgc gtctggaagg cggaagagaa aattgacaac
    50401 gcggttgtcc gctaaactgc tgtgaagtgc tgcaacctgt aggccgaata aggcggttac
    50461 gccgcatccg acatctaacg cccgagccgg ttgcctgatg cgacgctgaa gcgtcttatc
    50521 agcatacgcc acatccggca aaccatgccg gatgtggcac atcattacaa cgcaatatcc
    50581 gccacttctt tttgaacggg ttgcggtttc aactgcggtt tcggcgtact atctgctgcc
    50641 tgcggcttgt cgtatttcag ccccagcact tcgctggtgt actgcaactc gcgttctgtt
    50701 gccgctacat caccgttcag cttgcgtcca taagacggaa cgatcgcttt caacgtagcc
    50761 tgccattgcg ggctggaaac gcgatcgcca aatacttttt ccagcagatt caacataatc
    50821 ggcgcggcgg ttgacgcccc tggcgatgcc cccaggagcg cggcaatggt cccttgctgg
    50881 tcactgacga cttcagtacc cagacgcagt acgccacctt tatcggcatc acgcttgata
    50941 atctgcacgc gctgccccgc ttgccacaaa cgccagtcct cttttttcgc ctgcggatag
    51001 tactctttca acgcttcaaa acgatcctct tcactcaaca tcacctgact caccagatat
    51061 ttcaccagat cgaaattatc cagcccaacg tgcatcatcg gcatcacgtt agaggtggtg
    51121 gtggaactca tcagatccca taatgaaccg tttttgagga atttggttga gaaggtggca
    51181 aatggcccaa acagcactac gcgtttaccg tcaagaacac gggtatcgat atgcggaacc
    51241 gacatcggtg gtgcgccaac ggatgcttta ccgtaaacct tcgccagatg gtgattaacc
    51301 acgtccgggt tttccgaaac aaggaactgt ccacccaccg ggaaaccggc gtagtctttc
    51361 gcctccggaa tccccgattc ctgtaacagt ttcagcgccg caccgcccgc gccgataaag
    51421 acaaatttgg cgcggatatt ctgtgcagtg ccgtttttca gatcggcaac ggtgacggtc
    51481 cagctgttat cgtcattacg ctttagggcg cggacttcgc tgctgagttg cagcgagaag
    51541 ttagatttct tctgtaagga ggcgatcaac tggcgggtga tctcgccata gttcacatca
    51601 gtgccaattt ctgtgcgtgt tgccgctact ttctgttgcg gatcgcgccc ttccatcact
    51661 aacggtgccc actctttgat ctgcgcgtga tcttcagagt aacgcatacc gcgaaacagc
    51721 gagctttgtt gcaacgcggc gtaacgggcg cgcaggaaat taacgttatc ctcgccccag
    51781 acaaagctca tatgcggaac ggtattgata aatgaacgcg gagtacgcag cacaccgcgc
    51841 tcgacctggt gcgcccagaa ctggcgggaa atctgaaatg cttcgttaat ggcgactgct
    51901 ttttcaatac tgatgctgcc gtcggcattt tgcggggtgt aattgagttc catcagtgca
    51961 gaatgcccgg ttccggcgtt gttccagccg ttcgaactct cctgcgcgac accttccagg
    52021 cgctccacca tggtcatcga ccattcaggc tccagctcgc gtaaataggt ccccaacgtg
    52081 gcgctcataa tgccgccgcc aatcaacagt acatcagttt cctgctcctc ggacgctttc
    52141 gcttttgccg ccatcgaaac ggcattaagc cccacggcca tcgagaagag catggcagtc
    52201 acttttttca tcttgttaat gccttacttt tagtcgcttt attgcaggtg agatttgcgc
    52261 ggcatcaacg gtaacactta agtaacaata tttaaataat gtttataa
//
LOCUS       DADSYM010000008        51321 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2415
            SAMN08039579-rid11723173.denovo.008, whole genome shotgun sequence.
ACCESSION   DADSYM010000008 DADSYM010000000
VERSION     DADSYM010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039579
            Sequence Read Archive: SRR6362533
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 51321)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 51321)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980162.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 03:39:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,484
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 4,347
            CDSs (with protein)               :: 4,347
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 43 of 85
            Pseudo Genes (incomplete)         :: 37 of 85
            Pseudo Genes (internal stop)      :: 19 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..51321
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039579-rid11723173.denovo.008"
                     /strain="LGE2415"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8302 N 95.9628 W"
                     /collection_date="2015-08-24"
     gene            complement(138..1028)
                     /gene="rarD"
                     /locus_tag="J1Y83_000397"
     CDS             complement(138..1028)
                     /gene="rarD"
                     /locus_tag="J1Y83_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter RarD"
                     /protein_id="HBA7628583.1"
                     /translation="MDAKQTRQGVLLALAAYFIWGIAPAYFKLIYYVPADEILTHRVI
                     WSFFFMVVLMSICRQWSYLKTLIQTPQKIFMLAVSAVLIGGNWLLFIWAVNNHHMLEA
                     SLGYFINPLVNIVLGMIFLGERFRRMQWLAVILAICGVLVQLWTFGSLPIIALGLAFS
                     FAFYGLVRKKIAVEAQTGMLIETMWLLPVAAIYLFAIADSSTSHMGQNPMSLNLLLIA
                     AGIVTTVPLLCFTAAATRLRLSTLGFFQYIGPTLMFLLAVTFYGEKPGADKMVTFAFI
                     WVALAIFVMDAIYTQHRTSK"
     gene            complement(1080..1547)
                     /locus_tag="J1Y83_000398"
     CDS             complement(1080..1547)
                     /locus_tag="J1Y83_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001350877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HBA7628584.1"
                     /translation="MSAVLTAEQALKLVGEMFVYHMPFNRALGMELERYEKEFAQLAF
                     KNQPMMVGNWAQSILHGGVIASALDVAAGLVCVGSTLTRHETISEDELRQRLSRMGTI
                     DLRVDYLRPGRGERFTATSSLLRAGNKVAVARVELHNEEQLYIASATATYMVG"
     gene            1711..2580
                     /gene="pldA"
                     /locus_tag="J1Y83_000399"
     CDS             1711..2580
                     /gene="pldA"
                     /locus_tag="J1Y83_000399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase A"
                     /protein_id="HBA7628585.1"
                     /translation="MRTLQGWLLPVFMLPMAVYAQEATVKEVHDAPAVRGSIIANMLQ
                     EHDNPFTLYPYDTNYIIYTQTSDLNKEAIASYDWAENARKDEVKFQLSLAFPLWRGIL
                     GPNSVLGASYTQKSWWQLSNSDESSPFRETNYEPQLFLGFATDYNFAGWTLRDVEMGY
                     NHDSNGRSDPTSRSWNRLYTRLMAENGNWLVEVKPWYVVGNTDDNPDITKYMGYYQLK
                     IGYHLGDAVLSAKGQYNWNTGYGGAELGLSYPITKHVRLYTQVYSGYGESLIDYNFNQ
                     TRVGVGVMLNDLF"
     gene            2705..4534
                     /gene="recQ"
                     /locus_tag="J1Y83_000400"
     CDS             2705..4534
                     /gene="recQ"
                     /locus_tag="J1Y83_000400"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026263.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HBA7628586.1"
                     /translation="MAQAEVLNLESGAKQVLQETFGYQQFRPGQEEIIDTVLSGRDSL
                     VVMPTGGGKSLCYQIPALLLNGLTVVVSPLISLMKDQVDQLQANGVAAACLNSTQTRE
                     QQLEVMTGCRTGQISLLYIAPERLMLDNFLEHLAHWNPVLLAVDEAHCISQWGHDFRP
                     EYAALGQLRQRFPTLPFMALTATADDTTRQDIVRLLGLNDPLIQISSFDRPNIRYMLM
                     EKFKPLDQLMRYVQEQRGKSGIIYCNSRAKVEDTAARLQSKGISAAAYHAGLENNVRA
                     DVQEKFQRDDLQIVVATVAFGMGINKPNVRFVVHFDIPRNIESYYQETGRAGRDGLPA
                     EAMLFYDPADMAWLRRCLEEKPQGQLQDIERHKLNAMGAFAEAQTCRRLVLLNYFGEG
                     RQEPCGNCDICLDPPKQYDGSTDAQIALSTIGRVNQRFGMGYVVEVIRGANNQRIRDY
                     GHDKLKVYGMGRDKSHEHWVSVIRQLIHLGLVTQNIAQHSALQLTEAARPVLRGESSL
                     QLAVPRIVALKPKAMQKSFGGNYDRKLFAKLRKLRKSIADESNVPPYVVFNDATLIEM
                     AEQMPITASEMLSVNGVGMRKLERFGKPFMALIRAHVDGDDEE"
     gene            4598..5218
                     /gene="rhtC"
                     /locus_tag="J1Y83_000401"
     CDS             4598..5218
                     /gene="rhtC"
                     /locus_tag="J1Y83_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709629.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine export protein RhtC"
                     /protein_id="HBA7628587.1"
                     /translation="MLMLFLTVAMVHIVALMSPGPDFFFVSQTAVSRSRKEAMMGVLG
                     ITCGVMVWAGIALLGLHLIIEKMAWLHTLIMVGGGLYLCWMGYQMLRGALKKEVVSAP
                     APQVELAKSGRSFLKGLLTNLANPKAIIYFGSVFSLFVGDNVGTTERWGIFALIIVET
                     LAWFTVVASLFALPQMRRGYQRLAKWIDGFAGALFAGFGIHLIISR"
     gene            complement(5279..5899)
                     /gene="rhtB"
                     /locus_tag="J1Y83_000402"
     CDS             complement(5279..5899)
                     /gene="rhtB"
                     /locus_tag="J1Y83_000402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine/homoserine lactone efflux protein"
                     /protein_id="HBA7628588.1"
                     /translation="MTLEWWFAYLLTSIILSLSPGSGAINTMTTSLNHGYRGAVASIA
                     GLQTGLAIHIVLVGVGLGTLFSRSVIAFEVLKWAGAAYLIWLGIQQWRAAGAIDLKSL
                     ASTQSRRHLFQRAVFVNLTNPKSIVFLAALFPQFIMPQQPQLMQYIVLGVTTIVVDII
                     VMIGYATLAQRIALWIKGPKQMKALNKIFGSLFMLVGALLASARHA"
     gene            6010..7032
                     /gene="pldB"
                     /locus_tag="J1Y83_000403"
     CDS             6010..7032
                     /gene="pldB"
                     /locus_tag="J1Y83_000403"
                     /EC_number="3.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipase L2"
                     /protein_id="HBA7628589.1"
                     /translation="MFQQQKDWETRENAFAAFTMGPLTDFWRQRDEAEFTGVDDIPVR
                     FVRFRAQHHDRVVVICPGRIESYVKYAELAYDLFHLGFDVLIIDHRGQGRSGRLLGDP
                     HLGHVNRFNDYVDDLAAFWQQEVQPGPWRKRYILAHSMGGAISTLFLQRHPGVCDAIA
                     LTAPMFGIVIRMPSFMARQILNWAEAHPRFRDGYAIGTGRWRALPFAINVLTHSRQRY
                     RRNLRFYADDPTIRVGGPTYHWVRESILAGEQVLAGAGDDATPTLLLQAEEERVVDNR
                     MHDRFCELRTVAGHPVEGGRPLVIKGAYHEILFEKDAMRSVALHAIVDFFNRHNSPSG
                     NRSTEV"
     gene            7040..7840
                     /gene="yigL"
                     /locus_tag="J1Y83_000404"
     CDS             7040..7840
                     /gene="yigL"
                     /locus_tag="J1Y83_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar/pyridoxal phosphate phosphatase YigL"
                     /protein_id="HBA7628590.1"
                     /translation="MYQVVASDLDGTLLSPDHTLSPYAKETLKLLTARGINFVFATGR
                     HHVDVGQIRDNLEIKSYMITSNGARVHDLDGNLIFAHNLDRDIASDLFGVVNDNPDII
                     TNVYRDDEWFMNRHRPEEMRFFKEAVFNYALYEPGLLEPEGVSKVFFTCDSHEKLLPL
                     EQAINARWGDRVNVSFSTLTCLEVMAGGVSKGHALEAVAKKLGYSLQDCIAFGDGMND
                     AEMLSMAGKGCIMGSAHQRLKDLHPELEVIGTNAEDAVPHYLRKLYLS"
     gene            7916..8815
                     /gene="bioP"
                     /locus_tag="J1Y83_000405"
     CDS             7916..8815
                     /gene="bioP"
                     /locus_tag="J1Y83_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter"
                     /protein_id="HBA7628591.1"
                     /translation="MALLIITTILWAFSFSFYGEYLAGHVDSYFAVLVRVGLAALVFL
                     PFLRTRGNSLKTVGLYMLVGAMQLGVMYMLSFRAYLYLTVSELLLFTVLTPLYITLIY
                     DIMSKRRLRWGYAFSALLAVIGAGIIRYDQVTDHFWTGLLLVQLSNITFAIGMVGYKR
                     LMETRPMPQHNAFAWFYLGAFLVAVIAWFLLGNAQKMPQTTLQWGILVFLGVVASGIG
                     YFMWNYGATQVDAGTLGIMNNMHVPAGLLVNLAIWHQQPHWPTFITGALVILASLWVH
                     RKWVAPRSSQTADDRRRDCALNE"
     gene            complement(8703..9656)
                     /gene="metR"
                     /locus_tag="J1Y83_000406"
     CDS             complement(8703..9656)
                     /gene="metR"
                     /locus_tag="J1Y83_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MetR"
                     /protein_id="HBA7628592.1"
                     /translation="MIEVKHLKTLQALRNCGSLAAAAATLHQTQSALSHQFSDLEQRL
                     GFRLFVRKSQPLRFTPQGEILLQLANQVLPQISQALQACNEPQQTRLRIAIECHSCIQ
                     WLTPALENFHKNWPQVEMDFKSGVTFDPQPALQQGELDLVMTSDILPRSGLHYSPMFD
                     YEVRLVLAPDHPLAAKTRITPEDLASETLLIYPVQRSRLDVWRHFLQPAGVSPSLKSV
                     DNTLLLIQMVAARMGIAALPHWVVESFERQGLVVTKTLGEGLWSRLYAAVRDGEQRQP
                     ITEAFIRSARNHACDHLPFVKSAERPTYDAPTVRPGSPARL"
     gene            9774..12035
                     /gene="metE"
                     /locus_tag="J1Y83_000407"
     CDS             9774..12035
                     /gene="metE"
                     /locus_tag="J1Y83_000407"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HBA7628593.1"
                     /translation="MTILNHTLGFPRVGLRRELKKAQESYWAGNSTREELLAVGRELR
                     ARHWDQQKQAGIDLLPVGDFAWYDHVLTTSLLLGNVPPRHQNKDGSVDIDTLFRIGRG
                     RAPTGEPAAAAEMTKWFNTNYHYMVPEFVKGQQFKLTWTQLLEEVDEALALGHKVKPV
                     LLGPITYLWLGKVKGEQFDRLSLLNDILPVYQQVLAELAKRGIEWVQIDEPALVLELP
                     QAWLDAYKPAYDALQGQVKLLLTTYFEGVTPNLDTITALPVQGLHVDLVHGKDDVAEL
                     HKRLPSDWLLSAGLINGRNVWRADLTEKYAQIKDIVGKRDLWVASSCSLLHSPIDLSV
                     ETRLDAEVKSWFAFALQKCHELALLRDALNSGDTAALAEWSAPIQARRHSTRVHNPAV
                     EKRLAAITAQDSQRANVYEVRAEAQRARFKLPAWPTTTIGSFPQTTEIRTLRLDFKKG
                     NLDANNYRTGIAEHIKQAIVEQERLGLDVLVHGEAERNDMVEYFGEHLDGFVFTQNGW
                     VQSYGSRCVKPPIVIGDVSRPAPITVEWAKYAQSLTDKPVKGMLTGPVTILCWSFPRE
                     DVSRETIAKQIALALRDEVADLEAAGIGIIQIDEPALREGLPLRRSDWDAYLQWGVEA
                     FRINAAVAKDDTQIHTHMCYCEFNDIMDSIAALDADVITIETSRSDMELLESFEEFDY
                     PNEIGPGVYDIHSPNVPSVEWIEALLKKAAKRIPAERLWVNPDCGLKTRGWPETRAAL
                     ANMVQAAQNLRRG"
     gene            12375..12932
                     /locus_tag="J1Y83_000408"
     CDS             12375..12932
                     /locus_tag="J1Y83_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000339043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HBA7628594.1"
                     /translation="MDAKLYITEIINELTFVKDSIREDDCTKLIDAIQQSQRVFCYGL
                     GRAGFSMKAFTMRLMHMGKEVYFLTETITPNFGPGDLFIVSSASGETAQLVALAKKAR
                     QLGGAVAVLTTNRHATITEFVDVIVQINAPSKNQKDSVFRSAQPMASLYEQALLVIAD
                     ALVMKMAAESGAPESELFKRHANLE"
     gene            12974..14482
                     /locus_tag="J1Y83_000409"
     CDS             12974..14482
                     /locus_tag="J1Y83_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001546794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HBA7628595.1"
                     /translation="MSKKITSLFNFKKLQKFSSALMLPIAILPAAGLILAIGISFNIE
                     LLANCGGLIFANLPLLFCVGIVIGLTGDGSSSLSAIISYMIMNATMGTQLGITAESVA
                     ASNGDYAMILGVPTLQTGVFGGLIVAIFVYLLYTRFHNIELPQFLGFFGGKRFVPIIT
                     AVAAIIVGYLLPWLWIPVQKGLASLSTLVTSGHNANFAAFIYAVGERSLIPFGLHHIW
                     NVPFYYNFGDYMTKSGQLVTGDIPVFFAQLRDGVPLTAGLFMTGRFPIMMFALPAAAL
                     AMYQEAKPERKALIKGLLLSGALTTFVTGITEPLEYAFLFAAPVLYIIHIFLYATSFV
                     LMNILNVHIGHPFAGGLIDFVLNGVMPNRTPWYLVFLAGAGYAAIYYTVFRVLIRKLN
                     LKTPGREDEEIAVATTLTREERPYQIIEAVGGFDNIEDVDACATRLRLALVDDKKVNE
                     KRLKELGAAGLVKLGDGGVQVIFGGKSQILRDEIKTVMSRPRPTEMTVACAN"
     gene            14533..16530
                     /gene="tkt"
                     /locus_tag="J1Y83_000410"
     CDS             14533..16530
                     /gene="tkt"
                     /locus_tag="J1Y83_000410"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005971397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HBA7628596.1"
                     /translation="MNSQLLANAIRMLSVDAIQKANSGHPGAPMGMADIAEVVWRRHL
                     RHNPKNPQWFNRDRYVQSNGHGSMLIYALLHLTGYDLSMDDIRDFRQLHSRTPGHPEY
                     GYTPGVETTTGPLGQGVANAVGMAIAEKALAAEFNKPGFNIVDHHTWLFLGDGCLMEG
                     ISHEACGLAGTLKLGNLIAIWDDNGISIDGHVEGWFAEDTAARFRAYGWHVIEGVDGH
                     DPEAVDAAVREAKSVTDKPSLLCCKTIIGFGSPNKANSHDCHGSALGADEVALVRERL
                     QWPYAPFEIPGEIYAAWDATEKGAQVQQEWDALFADYAKQWPELAAEFTRRMKGDLPA
                     GWAENMQKYVHDLQSHPAALATRQVSQKCLNHFADMLPELMGGSADLSPSNLTRHQKS
                     VDFTGENPAGNYISYGVREFGMSAIMNGLALHGGFIPYGGTFLMFMEYARNALRMAAL
                     MKIRSVFVYTHDTIGLGEDGPTHQPVEQLASLRLTPNMETWRGCDQVEVAVAWQQAIE
                     RKDGPTSLVLTRQPLAQQPRTAAQLAEIARGGYVLSDCDGQPEMILISAGSEIELVVS
                     AAKALTEEGRKVRVVSLPCTERFDNQDAAYKESVLPKAVRKRLAVEASIAGFWERYVG
                     LDGKVIGMTSFGESAPANVLFKHFGFTPENVLAQARELLNS"
     gene            complement(16560..17405)
                     /locus_tag="J1Y83_000411"
     CDS             complement(16560..17405)
                     /locus_tag="J1Y83_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020245455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HBA7628597.1"
                     /translation="MNNITVKSLIQSNYPTLHQAEKKVADYILSHAHEVVNYSVTELS
                     EKSHASEATIVRTCKKLGYQGYYHLKIALAKEVINPDNSYPDNTDFTDITSLATFLLK
                     KQAEDLIQSTQFFNAEVLESILKLLANCDTIFFFAAGNSNPLAVYGAYKFSQLGLKTV
                     VHVSPEMQINAAYSMGKRDLAIGISNSGSTNLMVDIFKVVKERGAKSVCITNYIKSPL
                     SKLSTHQLNTAVSDKIFFEAFDSTRVPAMGVIDMLVLLFMYKNKAHYEKYRTREEFLG
                     SRFKG"
     gene            complement(17605..18510)
                     /locus_tag="J1Y83_000412"
     CDS             complement(17605..18510)
                     /locus_tag="J1Y83_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001566491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBA7628598.1"
                     /translation="MFISDETLRVIHLVARHQSITTAAEQLNKVPSAISYTIKKAEES
                     LGVELFIRKGRYIELSPAGSYFIEHSKTILGDLEALKRNTVLIHDGIERELTIAVNNI
                     IPGDLLVAFIRDFERQFTSTTLTVDLEVYNGCWDALYSKRADLVYGAPHAVPSSEGII
                     SEPVGQMEWDFVVSPLHPLAAKRHPLENSELRHYPAVCIRDTSVNFPPMQAWLLEGQK
                     PIFVPDFATAIALIEQNVGIGYIPHHLALPLLNSGKLLKKPMREHKHATKLFLAARSD
                     GMGKACQWCIEYLRNPQLMTRFVFN"
     gene            complement(18588..19811)
                     /locus_tag="J1Y83_000413"
     CDS             complement(18588..19811)
                     /locus_tag="J1Y83_000413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020234945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytosine permease"
                     /protein_id="HBA7628599.1"
                     /translation="MRKKEENLNTASGLRIAMILLGIAVTPVLLSSSSLGNQLSSSSL
                     ISVVLLGGVILTLLSAITISVGEKARLPTYGIVKYSFGEKGAIAINILMAISLFGWIA
                     VTANMFGHSVHDLLAQHGLEVPLALLVAAGCVIFVASTAFGFAVLGKIAQVAVPVIAL
                     VLCYILYVATHTEVAVPAAIVEMNTGVAVSTVVGTIIVLVATLPDFGSFVHNRKHALI
                     AAGVTFLAAYPLLYWAGATPSAISGQGSLLGAMAVFGAVLPAALLLIFACVTGNAGNM
                     FQGTLVVSTLLTRFPKWQITVALGILSAIVGSMDIMAWFIPFLLFLGIATPPVAGIYI
                     ADFFLYRRNGYQESVLAQESQIKVLTFAAWIIGAAVGFMTVKGLFTLTTIPSVDSILV
                     ACIAYAILSRASQHR"
     gene            complement(19837..20226)
                     /locus_tag="J1Y83_000414"
     CDS             complement(19837..20226)
                     /locus_tag="J1Y83_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000214032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA amidotransferase"
                     /protein_id="HBA7628600.1"
                     /translation="MTTTLLAVNGTLMRGLELNPNMQKAGGIFVREDRTDAHYRLWSI
                     NDRHPGMIRVNEGGTHVDVEIWQLPLASFAALLMSEPAGLAIGKIKLADGSEVLGVLA
                     ENWLTEGQREITELGSWRKYTGHFHTV"
     gene            complement(20243..21199)
                     /gene="arcC"
                     /locus_tag="J1Y83_000415"
     CDS             complement(20243..21199)
                     /gene="arcC"
                     /locus_tag="J1Y83_000415"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001606533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HBA7628601.1"
                     /translation="MVKPLAVVAVGGNALIQDEQRNSIPDQYVAVMESVQHIVDMVEA
                     GWDLVLTHGNGPQVGFILRRSELASNEVSPVPLDYAVGDTQGAIGYMFQKALHNELAR
                     RGINKPVIALVTQTRVSPHDDAFASPSKPIGAFLDEATAQQRQQQLGWTLMEDAGRGW
                     RRTVPSPAPLEIIEHDTIAHLVRQGYLVIACGGGGIPVVRDGQQLKGVEAVIDKDLAS
                     ALLASQLGADLLVIPTGVEKMAINFGTPQQQWLDAIRVAEAQTLLREGQFGVGSMQPK
                     VEAIVDFINASQQQGKQASGLITSPQTIKAALAHQSGTWITL"
     gene            complement(21192..22616)
                     /locus_tag="J1Y83_000416"
     CDS             complement(21192..22616)
                     /locus_tag="J1Y83_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001093935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1116 domain-containing protein"
                     /protein_id="HBA7628602.1"
                     /translation="MNTLFNQPLKVVNAGLHSFADNIQHAGGSAIALNWQPPAQGDID
                     AGLDLASLLRHPLVENANQIAMTRYLEAQPMLVDVMLAKEAIPAMAEQKRILHSGPPI
                     AWEEMCGPVKGAIIGAMLYEGWATSQQDAENQINAGEIDLAPCHHYHAVGPMAGIISP
                     SMPLWVVENKTNGHRTFSNFNEGLGKVLRFGANNDEVLNRLAWMRDELAPAMKAAIAQ
                     HGELELKPLMAQALHMGDEVHNRNAAATGLLIKRLLPALLACSLPQEHIQRVVAFITG
                     NDHFFLNLSMAACKAMMDAAANVPFSSMVTVMARNGVNFGIRISGTGDRWFQAPANAV
                     EGLFFPGFGVDDAAADLGDSAITETAGVGGFAMASSPAIVKFVGGTPVDATNNSRRMQ
                     AITLGGNPAFTLPALNFAPTAAGIDARKVTDRGILPVINTGIAHKQAGVGQIGAGITT
                     APMACFVAAVRALAEIVAKENHHG"
     gene            complement(22613..24172)
                     /gene="fdrA"
                     /locus_tag="J1Y83_000417"
     CDS             complement(22613..24172)
                     /gene="fdrA"
                     /locus_tag="J1Y83_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000865643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase FdrA"
                     /protein_id="HBA7628603.1"
                     /translation="MKVKYKVFSNLYQDSVSLMQISAQISKLPGIQQASVVMGTPNNL
                     EQLRDAGLGNEINASPNDLVIAVMGEEDICNEALVLAQQRLTSKPDDETDSGIKTPEK
                     VSLEMALEAEPEANLALISVPGDYAAAEAIKALNLGMNVMMFSDNVSIGQEKSIKTLA
                     RERQRIVMGPDCGTAIVNGIPLGFANVVKRGAIGVIGASGTGLQEVTCRIDQLGAGIS
                     QALGTGGHDLSEEIGGISMLFALDALAQDDETRVIVLISKPPSPIVARTILERAEACG
                     KPVVVNFLGANPHDLARPNITAATTLASAANIAVALLNAQPLPSVETDVPCDDLTMLQ
                     NACQRLPAHRQAIRGVFAGGTFCYEAQLICQQKGFSAASNTPVAGNRALANIWQSEDH
                     TLIDMGDDDFTRGKPHPMIDPTLRNQRLLNELNDSHTAVVLFDLVLGYGASTTPASEL
                     LDQLSHIDMNNAPLLIAHVCGTEADPQIRSQQIRALQNAGIIIASSNAQAALWASAVA
                     QTQLQKKGLNA"
     gene            complement(24267..25127)
                     /locus_tag="J1Y83_000418"
     CDS             complement(24267..25127)
                     /locus_tag="J1Y83_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001374637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2877 domain-containing protein"
                     /protein_id="HBA7628604.1"
                     /translation="MVQVTLCASSLGYLFPQNETQDLRLHSAFKHAVNLYSDAGTFIT
                     LLCAQTYLNLPDAARVWLPECWDWRREIAHSDPIQLTQGLLRTPRFCVALDNATLWQS
                     PFVGRMLTLEAFPLVFQHYPTMASQRLLFCLEHNVQSTLHLPDSLTHQGLAIMEHPDA
                     LERQVPQLIGFGKGLTPDGDDYLLGYLAALWLWQLPAPLADHQYRLQQAIDQHAHNTT
                     DISRHYLERALQGHFSEPICQLLAQLVGSASAMTIASCAEQVMQFGATSGVDCLAGML
                     HGFRTLNTMN"
     gene            complement(25133..25801)
                     /locus_tag="J1Y83_000419"
     CDS             complement(25133..25801)
                     /locus_tag="J1Y83_000419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HBA7628605.1"
                     /translation="MTQSIFQAQPFELPFDPRTTALVMIDMQRDFVEAGGFGEALGND
                     VSLVRTAIAPCTEVLAAARQKGIMVIHTREGHRADLSDCPPAKLTRGGKTFIGEPGPM
                     GRILVRGEAGHDIIPELYPVAGEPVIDKPGKGAFYQTDLHLILQNHGIKTLIVCGVTT
                     EVCVTTTVREANDRGYECIIPEDCVGSYFPEFQKYALEMIKAQGAIFGWVTDSKAIIA
                     GLEG"
     gene            complement(26058..26867)
                     /locus_tag="J1Y83_000420"
     CDS             complement(26058..26867)
                     /locus_tag="J1Y83_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HBA7628606.1"
                     /translation="MATTQQSGFAPAASPLASTIVQTPDDAIVAGFTSIPSQGDNMPA
                     YHARPKQSDGPLPVVIVVQEIFGVHEHIRDICRRLALEGYLAIAPELYFREGDPNDFA
                     DIPTLLSGLVAKVPDSQVLADLDHVASWASRNGGDVHRLMITGFCWGGRITWLYAAHN
                     PQLKAAVAWYGKLTGDKSLNSPKQPVDIATDLNAPVLGLYGGQDNSIPQESVETMRQA
                     LRAANAKAEIIVYPDAGHAFNADYRPSYHAESAKDGWQRMLEWFTQYGVKK"
     gene            27129..27890
                     /gene="udp"
                     /locus_tag="J1Y83_000421"
     CDS             27129..27890
                     /gene="udp"
                     /locus_tag="J1Y83_000421"
                     /EC_number="2.4.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine phosphorylase"
                     /protein_id="HBA7628607.1"
                     /translation="MSKSDVFHLGLTKNDLQGATLAIVPGDPDRVEKIAALMDKPVKL
                     ASHREFTTWRAELDGKPVIVCSTGIGGPSTSIAVEELAQLGIRTFLRIGTTGAIQPHI
                     NVGDVLVTTASVRLDGASLHFAPLEFPAVADFECTTALVEAAKSIGATTHVGVTASSD
                     TFYPGQERYDTYSGRVVRHFKGSMEEWQAMGVMNYEMESATLLTMCASQGLRAGMVAG
                     VIVNRTQQEIPNAETMKQTESHAVKIVVEAARRLL"
     gene            complement(27928..29418)
                     /locus_tag="J1Y83_000422"
     CDS             complement(27928..29418)
                     /locus_tag="J1Y83_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001606539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylate ABC transporter permease"
                     /protein_id="HBA7628608.1"
                     /translation="MFESELLTQGFSTLLNNPQALLFATFGVMLGIVIGALPGLTATM
                     GVAILLPFTYGMEPVSGLLMICGVFFGGVYGGSITAILLKIPGTPAAAATAIDGYELT
                     KQGKAGLALSAATFSSFSGGTLSIIVLMFLSPVLASWALKFSASESFALATFGLSIIA
                     SISGESLIKGLIAGVGGLLIATIGLDPMGGFPRFTGGFVELMNVPFIPVMIGLFAASE
                     AFRSMEQNQQIRRGAKVAIGSLLLPWQTLRRIALTILRSSGLGVFIGMIPGAGADIAA
                     FVAYNETRRFSKTPENFGKGEIKAVASCEAGANGCTGGALLPMLTLGIPGDAVTAIML
                     GALTLQGMQPGPLMFTDHGDMVYTLFVGMIFCYFMLLVLGLLSLKVIGNVVKIPGNIL
                     TPMILALCVVGTYALNNSLFDVGIMLIAGVVGYFMQKGGYPASPVVLALIMGPMAESN
                     FRRALSLSGGSLDFLYTRPITLALLTLAAFTLLTPIIRKIMRLRRQ"
     gene            complement(29431..29937)
                     /locus_tag="J1Y83_000423"
     CDS             complement(29431..29937)
                     /locus_tag="J1Y83_000423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001596577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter TctB
                     family protein"
                     /protein_id="HBA7628609.1"
                     /translation="MNTKQSVAQLAVPHRKRLSSTMVVALLLCVVAGAVMINAADFPA
                     TAIETDPGASAFPTFYACALIVLAVLLVIRDLLQAKPASCANAQEKPAFRKTATGIAA
                     TAFYIVAMSYCGYLITTPVFLIVIMTLMGYRRWVLTPGIALLLTAILWLLFVEALQVP
                     LPVGTFFE"
     gene            complement(29954..30928)
                     /locus_tag="J1Y83_000424"
     CDS             complement(29954..30928)
                     /locus_tag="J1Y83_000424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HBA7628610.1"
                     /translation="MIKHLTLPKKMAAMVLLAGAALSIAPVQAASWPTKQIELVVPYA
                     AGGGTDLVARAFADAAKNHLPVSIGVINKPGGGGAIGLSEIAAARPNGYKIGLGTVEL
                     TTLPSLGMVRFKTSDFKPIARLNADPAAITVRADAPWNSYEEFMTYAKANPGKVRIGN
                     SGTGAIWHLAAAALEDKTGAKFSHVPYDGAAPAITGLLGGHIEAVSVSPGEVINHVNG
                     GKLKTLVVMADERMKTMPDVPTLKEKGVDLSIGTWRGLIVSQKTPQDVVDVLAKAAKE
                     TAEEPAFQDALQKLNLNYAWLDAASFQTQISEQEKYFDELLTRLGLKK"
     gene            complement(30953..31582)
                     /locus_tag="J1Y83_000425"
     CDS             complement(30953..31582)
                     /locus_tag="J1Y83_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001116470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxy-6-phosphogalactonate aldolase"
                     /protein_id="HBA7628611.1"
                     /translation="MPFNTLLQKTGLVAILRGVKPDEIVAIGEKLYAAGFRLIEIPMN
                     SPEALQSISLLRDALPKDCLVGAGTVLSVEQVVAVKEAGGQIIVMPHCDTAVIRRARA
                     LGMYCAPGVATPTEAFAAIEHGANAIKLFPAEQITPEVTKAWRAVIPQSVPMLPVGGI
                     TPETMARYLSHGANGFGLGSALYHPGMTPEQVYENAVLFMNAWNNLNSN"
     gene            complement(31572..32576)
                     /locus_tag="J1Y83_000426"
     CDS             complement(31572..32576)
                     /locus_tag="J1Y83_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001117527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxygalactonokinase"
                     /protein_id="HBA7628612.1"
                     /translation="MPGAPSFIALDWGTSSLRAWRFGESPNPQEKREFPWGIMKLPSQ
                     AATREDAFHDTFLRVCGDWLAQTPCPVLACGMLGSAQGWQSAAYLPCPVTLEGLAKQL
                     TPVIHQQQTMLHIIPGVIKEGEMPEVMRGEETQIFGAISMEPALQNAIHQGMPVLIGL
                     PGTHAKWAVVENNTITDFRTFMTGELFDVLSRHSILGATMHPGDEPHWDAFTHGLTAA
                     QEHHQTGLLSTLFSTRSRLLTSNLTSSSQGDYLSGLLIGHELCGLASSLLRDLPATTP
                     IALIGSANLNSRYSQAFSHVFPDRQIHAIPNATEQGLWRIAHAAGLLSTNARECTHAI
                     "
     gene            complement(32600..33367)
                     /locus_tag="J1Y83_000427"
     CDS             complement(32600..33367)
                     /locus_tag="J1Y83_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000852399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HBA7628613.1"
                     /translation="MAETSSTTAGAQTLLRGLAVLNAVYNGCHDLKSIGEFTGTTRST
                     THRLVTVLVEQRYLRHVPTQGYQLGAKLIEFGARALESTSLYEIAQPVLQRLARYTLD
                     TVHLGIVEGDEVLYLEKINSQRGLEMRSRPGHRMPLAITGIGKALILNRTEEEWRTLF
                     KTCGDETKLGAFIQNMRRYAASGFAFDLEENEPTIRCVAAPVYNARDEIVAAISVAST
                     TTYMSLARLEELAPYVKSCAEEISAELGWGKHVRKDK"
     gene            33584..35011
                     /gene="rmuC"
                     /locus_tag="J1Y83_000428"
     CDS             33584..35011
                     /gene="rmuC"
                     /locus_tag="J1Y83_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HBA7628614.1"
                     /translation="MDFSIIVYAIIALVSVVIGWLFASYQHAQQKAEQLAEREEMVAE
                     LSAAKQQITQSEHWRAECELLNNEVRSLQSINTSLEADLREVTTRMEAAQQHADDKIR
                     QMINSEQRLSEQFENLANRIFEHSNRRVDEQNRQSLNSLLSPLREQLDGFRRQVQDSF
                     GKEAQERHTLTHEIRNLQQLNAQMAQEAINLTRALKGDNKTQGNWGEVVLTRVLEASG
                     LREGYEYETQVSIENDARSRMQPDVIVRLPQGKDVVIDAKMTLVAYERYFNAEDDYSR
                     ESALQEHIASVRNHIRLLGRKDYQQLSGLRTLDYVLMFIPVEPAFLLALDRQPELITE
                     ALKNNIMLVSPTTLLVALRTIANLWRYEHQSRNAQHIADRASKLYDKMRLFVDDMSAI
                     GQSLDKAQDNYRQAMKKLSSGRGNVLAQAEAFRGLGVEIKREINPDLAEQAVSQDEEY
                     RLRSVPEQPNDEAYQRDDEYNQQSR"
     gene            35106..35861
                     /gene="ubiE"
                     /locus_tag="J1Y83_000429"
     CDS             35106..35861
                     /gene="ubiE"
                     /locus_tag="J1Y83_000429"
                     /EC_number="2.1.1.163"
                     /EC_number="2.1.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional demethylmenaquinone
                     methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol
                     methylase UbiE"
                     /protein_id="HBA7628615.1"
                     /translation="MVDKSQETTHFGFQTVAKEQKADMVAHVFHSVASKYDVMNDLMS
                     FGIHRLWKRFTIDCSGVRRGQTVLDLAGGTGDLTAKFSRLVGETGKVVLADINESMLK
                     MGREKLRNIGVIGNVEYVQANAEALPFPDNTFDCITISFGLRNVTDKDKALRSMYRVL
                     KPGGRLLVLEFSKPIIEPLSKAYDAYSFHVLPRIGSLVANDADSYRYLAESIRMHPDQ
                     DTLKTMMQDAGFESVDYYNLTAGVVALHRGYKF"
     gene            35875..36480
                     /gene="ubiJ"
                     /locus_tag="J1Y83_000430"
     CDS             35875..36480
                     /gene="ubiJ"
                     /locus_tag="J1Y83_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis protein UbiJ"
                     /protein_id="HBA7628616.1"
                     /translation="MPFKPLVTAGIESLLNTFLYRSPALKTARSRLLGKVLRVEVKGF
                     STSLILVFSERQVDVLGEWAGDADCTVIAYASVLPKLRDRQQLAALIRSGELEVQGDI
                     QVVQNFVALADLAEFDPAELLAPYTGDIAAEGISKALRGGAKFLHHGIKRQQRYVAEA
                     ITEEWRMAPGPLEVAWFAEETAAVERAVDALTKRLEKLEAK"
     gene            36477..38117
                     /gene="ubiB"
                     /locus_tag="J1Y83_000431"
     CDS             36477..38117
                     /gene="ubiB"
                     /locus_tag="J1Y83_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis regulatory protein
                     kinase UbiB"
                     /protein_id="HBA7628617.1"
                     /translation="MTPGEVRRLYFIIRTFLSYGLDELIPKMRITLPLRLWRYSLFWM
                     PNRHKDKPLGERLRLALQELGPVWIKFGQMLSTRRDLFPPHIADQLALLQDKVAPFDG
                     KLAKQQIEAAMGGLPVEAWFDDFEIKPLASASIAQVHTARLKSNGKEVVIKVIRPDIL
                     PVIKADLKLIYRLARWVPRLLPDGRRLRPTEVVREYEKTLIDELNLLRESANAIQLRR
                     NFEDSPMLYIPEVYPDYCSEGMMVMERIYGIPVSDVATLEKNGTNMKLLAERGVQVFF
                     TQVFRDSFFHADMHPGNIFVSYEHPENPKYIGIDCGIVGSLNKEDKRYLAENFIAFFN
                     RDYRKVAELHVDSGWVPPDTNVEEFEFAIRTVCEPIFEKPLAEISFGHVLLNLFNTAR
                     RFNMEVQPQLVLLQKTLLYVEGVGRQLYPQLDLWKTAKPFLESWIKDQVGIPALVRAF
                     KEKAPFWVEKMPELPELVYDSLRQGKYLQHSVDKIARELQSNHVRQGQSRYFLGIGAT
                     LVLSCTFLLVSRPEWGLMPGWLMAGGLIAWFVGWRKTR"
     gene            38196..38465
                     /gene="tatA"
                     /locus_tag="J1Y83_000432"
     CDS             38196..38465
                     /gene="tatA"
                     /locus_tag="J1Y83_000432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatA"
                     /protein_id="HBA7628618.1"
                     /translation="MGGISIWQLLIIAVIVVLLFGTKKLGSIGSDLGASIKGFKKAMS
                     DDEPKQDKISQDADFTAKTIADKQADTNQEQAKTEDAKRHDKEQV"
     gene            38469..38984
                     /gene="tatB"
                     /locus_tag="J1Y83_000433"
     CDS             38469..38984
                     /gene="tatB"
                     /locus_tag="J1Y83_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatB"
                     /protein_id="HBA7628619.1"
                     /translation="MFDIGFSELLLVFIIGLVVLGPQRLPVAVKTVAGWIRALRSLAT
                     TVQNELTQELKLQEFQDSLKKVEKASLTNLTPELKASMDELRQAAESMKRSYVANDPE
                     KASDEAHTIHNPVVKDNETAHEGVTPAAAQTQASSPEQKPETTPEPVVKPAADAEPKT
                     AAPSPSSSDKP"
     gene            38987..39763
                     /gene="tatC"
                     /locus_tag="J1Y83_000434"
     CDS             38987..39763
                     /gene="tatC"
                     /locus_tag="J1Y83_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatC"
                     /protein_id="HBA7628620.1"
                     /translation="MSVEDTQPLITHLIELRKRLLNCIISVIVIFLCLVYFANDIYHL
                     VSAPLIKQLPQGSTMIATDVASPFFTPIKLTFMVSLILSAPVILYQVWAFIAPALYKH
                     ERRLVVPLLVSSSLLFYIGMAFAYFVVFPLAFGFLANTAPEGVQVSTDIASYLSFVMA
                     LFMAFGVSFEVPVAIVLLCWMGITSPEDLRKKRPYVLVGAFVVGMLLTPPDVFSQTLL
                     AIPMYCLFEIGVFFSRFYVGKGRNREEENDAEAESEKTEE"
     gene            39805..40587
                     /gene="tatD"
                     /locus_tag="J1Y83_000435"
     CDS             39805..40587
                     /gene="tatD"
                     /locus_tag="J1Y83_000435"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' ssDNA/RNA exonuclease TatD"
                     /protein_id="HBA7628621.1"
                     /translation="MFDIGVNLTSSQFAKDRDDVVARAFDAGVNGLLITGTNLRESQQ
                     AQKLARQYSSCWSTAGVHPHDSSQWQAVTEEAIIELAAQPEVVAIGECGLDFNRNFST
                     PEEQELAFVAQLRIAAELNMPVFMHCRDAHERFMTLLEPWLDKLPGAVLHCFTGTREE
                     MQACVAHGIYIGITGWVCDERRGLELRELLPLIPAEKLLIETDAPYLLPRDLTPKPSS
                     RRNEPAHLPHILQRIAHWRGEDAAWLAATTDANVKTLFGIAF"
     gene            complement(40584..41072)
                     /gene="rfaH"
                     /locus_tag="J1Y83_000436"
     CDS             complement(40584..41072)
                     /gene="rfaH"
                     /locus_tag="J1Y83_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription/translation regulatory transformer
                     protein RfaH"
                     /protein_id="HBA7628622.1"
                     /translation="MQSWYLLYCKRGQLQRAQEHLERQAVNCLAPMITLEKIVRGKRT
                     AVSEPLFPNYLFVEFDPEVIHTTTINATRGVSHFVRFGASPAIVPSAVIHQLSVYKPK
                     DIVDPSTPYPGDKVIITEGAFEGFQAIFTEPDGEARSMLLLNLINKEIKHSVKNTEFR
                     KL"
     gene            41239..42732
                     /gene="ubiD"
                     /locus_tag="J1Y83_000437"
     CDS             41239..42732
                     /gene="ubiD"
                     /locus_tag="J1Y83_000437"
                     /EC_number="4.1.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-polyprenylbenzoate decarboxylase"
                     /protein_id="HBA7628623.1"
                     /translation="MDAMKYNDLRDFLTLLEQQGELKRITLPVDPHLEITEIADRTLR
                     AGGPALLFENPKGYSMPVLCNLFGTPKRVAMGMGQEDVSALREVGKLLAFLKEPEPPK
                     GFRDLFDKLPQFKQVLNMPTKRLRGAPCQQKIVSGDDVDLNRIPIMTCWPEDAAPLIT
                     WGLTVTRGPHKERQNLGIYRQQLIGKNKLIMRWLSHRGGALDYQEWCAAHPGERFPVS
                     VALGADPATILGAVTPVPDTLSEYAFAGLLRGTKTEVVKCISNDLEVPASAEIVLEGY
                     IEQDETAPEGPYGDHTGYYNEVDSFPVFTVTHITQREDAIYHSTYTGRPPDEPAVLGV
                     ALNEVFVPILQKQFPEIVDFYLPPEGCSYRLAVVTIKKQYAGHAKRVMMGVWSFLRQF
                     MYTKFVIVCDDDVNARDWNDVIWAITTRMDPARDTVLVENTPIDYLDFASPVSGLGSK
                     MGLDATNKWPGETQREWGRPIKKDPDVVAHIDAIWDELAIFNNGKSA"
     gene            42778..43479
                     /gene="fre"
                     /locus_tag="J1Y83_000438"
     CDS             42778..43479
                     /gene="fre"
                     /locus_tag="J1Y83_000438"
                     /EC_number="1.5.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-flavin reductase"
                     /protein_id="HBA7628624.1"
                     /translation="MTTLSCKVTSVEAITDTVYRVRIVPDAAFSFRAGQYLMVVMDER
                     DKRPFSMASTPDEKGFIELHIGASEINLYAKAVMDRILKDHQIVVDIPHGEAWLRDDE
                     ERPMILIAGGTGFSYARSILLTALARNPNRDITIYWGGREEHHLYDLCELEALSLKHP
                     GLQVVPVVEQPEAGWRGRTGTVLTAVLQDHGTLAEHDIYIAGRFEMAKIARDLFCSER
                     NAREDRLFGDAFAFI"
     gene            complement(43751..44914)
                     /gene="fadA"
                     /locus_tag="J1Y83_000439"
     CDS             complement(43751..44914)
                     /gene="fadA"
                     /locus_tag="J1Y83_000439"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase FadA"
                     /protein_id="HBA7628625.1"
                     /translation="MEQVVIVDAIRTPMGRSKGGAFRNVRAEDLSAHLMRSLLARNPA
                     LEAAALDDIYWGCVQQTLEQGFNIARNAALLAEVPHSVPAVTVNRLCGSSMQALHDAA
                     RMIMTGDAQACLVGGVEHMGHVPMSHGVDFHPGLSRNVAKAAGMMGLTAEMLARMHGI
                     SREMQDAFAARSHARAWAATQSGAFKNEIIPTGGHDADGVLKQFNYDEVIRPETTVEA
                     LATLRPAFDPVSGTVTAGTSSALSDGAAAMLVMSESRARELGLKPRARMRSMAVVGCD
                     PSIMGYGPVPASKLALKKAGLSASDIGVFEMNEAFAAQILPCIKDLGLMEQIDEKINL
                     NGGAIALGHPLGCSGARISTTLLNLMEHKDVQFGLATMCIGLGQGIATVFERV"
     gene            complement(44924..47113)
                     /gene="fadB"
                     /locus_tag="J1Y83_000440"
     CDS             complement(44924..47113)
                     /gene="fadB"
                     /locus_tag="J1Y83_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001490315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid oxidation complex subunit alpha FadB"
                     /protein_id="HBA7628626.1"
                     /translation="MLYKGDTLYLDWLEDGIAELVFDAPGSVNKLDTATVASLGEAIG
                     VLEQQSDLKGLLLRSNKAAFIVGADITEFLSLFLVPEEQLSQWLHFANSVFNRLEDLP
                     VPTIAAVNGYALGGGCECVLATDYRLATPDLRIGLPETKLGIMPGFGGSVRMPRMLGA
                     DSALEIIAAGKDVGADQALKIGLVDGVVKAEKLVEGAMAILRQAINGDLDWKAKRQPK
                     LEPLKLSKIEAAMSFTIAKGMVAQTAGKHYPAPITAVKTIEAAARFGREEALNLENKS
                     FVPLAHTNEARALVGIFLNDQYVKGKAKKLTKDVETPKQAAVLGAGIMGGGIAYQSAW
                     KGVPVVMKDINDKSLTLGMTEAAKLLNKQLERGKIDGLKLAGVISTIHPTLDYAGFDR
                     VDVVVEAVVENPKVKKAVLAETEQKVRPDTVLASNTSTIPISELANALERPENFCGMH
                     FFNPVHRMPLVEIIRGEKSSDETIAKVVAWASKMGKTPIVVNDCPGFFVNRVLFPYFA
                     GFSQLLRDGADFRKIDKVMEKQFGWPMGPAYLLDVVGIDTAHHAQAVMAAGFPQRMQK
                     DYRDAIDALFNANRFGQKNGLGFWRYKEDSKGKPEKEEDVVVDDLLAKVSQPKRDFSE
                     EEIIARMMIPMVNEVVRCLEEGIIATPAEADMALVYGLGFPPFHGGAFRWLDTLGSAK
                     YLDMAQQYQHLGPLYEVPEGMRNKARHNEPYYPPVEPARPVGDLKTA"
     gene            47303..48634
                     /gene="pepQ"
                     /locus_tag="J1Y83_000441"
     CDS             47303..48634
                     /gene="pepQ"
                     /locus_tag="J1Y83_000441"
                     /EC_number="3.4.13.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020801804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro dipeptidase"
                     /protein_id="HBA7628627.1"
                     /translation="MESLASLYKNHIATLQERTRDALARFKLDALLIHSGELFNVFLD
                     DHPYPFKVNPQFKAWVPVTQVPNCWLLVDGVNKPKLWFYLPVDYWHNVEPLPNSFWTE
                     DVEVIALPKADGIGSLLPAARGNIGYIGPVPERALQLGIEASNINPKGVIDYLHYYRS
                     FKTEYELACMREAQKMAVNGHRAAEEAFRSGMSEFDINIAYLTATGHRDTDVPYSNIV
                     ALNEHAAVLHYTKLDHQAPEEMRSFLLDAGAEYNGYAADLTRTWSAKSDNDYAQLVKD
                     VNDEQLALIATMKAGVSYVDYHIQFHQRIAKLLRKHQIITDMSEEAMVENDLTGPFMP
                     HGIGHPLGLQVHDVAGFMQDDSGTHLAAPAKYPYLRCTRILQPGMVLTIEPGIYFIES
                     LLAPWREGQFSKHFNWQKIEALKPFGGIRIEDNVVIHENNVENMTRDLKLA"
     gene            48634..49248
                     /locus_tag="J1Y83_000442"
     CDS             48634..49248
                     /locus_tag="J1Y83_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312803.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMPACT family protein"
                     /protein_id="HBA7628628.1"
                     /translation="MESWLIPAAPVMVVEEIKKSRFITLLAHTDGVEAAKAFVESVRA
                     EHPDARHHCVAWVAGAPDDSQQLGFSDDGEPAGTAGKPMLAQLMGSGVGEITAVVVRY
                     YGGILLGTGGLVKAYGGGVNQALRQLTTQRKTPLTEYTLQCEYSQLTGIEALLGQCDG
                     KIINSDYQAFVLLRVALPAAKVAEFSAKLADFSRGSLQLLAIEE"
     gene            49287..50738
                     /gene="trkH"
                     /locus_tag="J1Y83_000443"
     CDS             49287..50738
                     /gene="trkH"
                     /locus_tag="J1Y83_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkH"
                     /protein_id="HBA7628629.1"
                     /translation="MHFRAITRIVGLLVILFSGTMIIPGLVALIYRDGAGRAFTQTFF
                     VALAIGSMLWWPNRKEKGELKSREGFLIVVLFWTVLGSVGALPFIFSESPNLTITDAF
                     FESFSGLTTTGATTLVGLDSLPHAILFYRQMLQWFGGMGIIVLAVAILPILGVGGMQL
                     YRAEMPGPLKDNKMRPRIAETAKTLWLIYVLLTVACALALWFAGMDAFDAIGHSFATI
                     AIGGFSTHDASIGYFDSPTINTIIAIFLLISGCNYGLHFSLLSGRSLKVYWRDPEFRM
                     FIGVQFSLVVICTLVLWFHNVYSSALMTINQAFFQVVSMATTAGFTTDSIARWPLFLP
                     VLLLCSAFIGGCAGSTGGGLKVIRILLLFKQGNRELKRLVHPNAVYSIKLGNRALPER
                     ILEAVWGFFSAYALVFIVSMLAIIATGVDDFSAFASVVATLNNLGPGLGVVADNFTSM
                     NPVAKWILIANMLFGRLEVFTLLVLFTPTFWRE"
     gene            50750..51295
                     /gene="hemG"
                     /locus_tag="J1Y83_000444"
     CDS             50750..51295
                     /gene="hemG"
                     /locus_tag="J1Y83_000444"
                     /EC_number="1.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="menaquinone-dependent protoporphyrinogen IX
                     dehydrogenase"
                     /protein_id="HBA7628630.1"
                     /translation="MKTLILFSTRDGQTREIASYLASELKELGIQADVANVHRIEEPQ
                     WENYDRVVIGASIRYGHYHSAFQEFVKKHATRLNSMPSAFYSVNLVARKPEKRTPQTN
                     SYARKFLMSSQWRPDRCAVIAGALRYPRYRWYDRFMIKLIMKMSGGETDTRKEVVYTD
                     WEQVANFAREIAHLTDKPTLK"
BASE COUNT        12149 a        12947 c        13703 g        12522 t
ORIGIN      
        1 gaaatttatt aaagcaaatg aaaatataca acgacgtctg gaaattctgc ctttccttat
       61 gtgagtctcc tgtcagcatg gcaatatgcc cactctcatg caagagtcgg catatttttt
      121 cagaatatat ttatttttta tttggacgtt ctatgctgcg tataaatcgc atccatcaca
      181 aaaattgcca gcgccaccca aataaaggcg aaagttacca tcttatcggc acccggtttt
      241 tcaccgtaaa acgtcacagc cagcaggaac atcagcgtcg gaccaatgta ctggaaaaag
      301 cctaacgttg agagacgcag gcgtgtcgca gctgcagtaa aacacaacag tggcacggtg
      361 gtgacaatac cggcggcgat cagcagtaaa ttcagcgaca tcgggttttg ccccatatga
      421 ctggttgagc tgtcggcaat agcaaacagg taaatcgccg ccacgggcag caaccacata
      481 gtttcgatta acatgccggt ttgcgcttca acagcaatct tcttgcgcac caggccgtag
      541 aaagcaaaac taaacgccag ccccagcgcg atgatcggca gtgaaccaaa agtccacaac
      601 tgaaccagca ctccacatat cgccagaatc accgccagcc attgcatccg gcggaatcgc
      661 tcgccgagga aaatcatccc cagcacaatg ttcaccagcg ggttaataaa gtaaccaagg
      721 ctcgcttcca gcatatggtg attgttcacc gcccagataa acagtagcca gttgccgcca
      781 atcagcacgg cagagactgc cagcataaaa attttctgtg gcgtctgaat cagcgttttt
      841 aaataggacc actggcggca aatgctcatc agcaccacca taaagaaaaa cgaccagatc
      901 acgcgatgcg tcaggatctc atcggcgggc acgtagtaaa tcagtttgaa gtacgctggc
      961 gcaatacccc aaataaaata agcggcaaga gcgagtaata cgccctgccg cgtttgtttt
     1021 gcatccatcg ggaatactca tttttaattg gtaacagcag tttacctgct tttatgtctt
     1081 caacctacca tataggtggc ggtggcactg gcaatataaa gctgttcttc attgtgtaat
     1141 tcaacgcggg cgacggcgac tttattgcct gcacgcaaca ggctactggt agcagtaaaa
     1201 cgctcgcctc tgcctgggcg cagataatcg acgcgaagat caatggtccc catccgcgat
     1261 agccgctggc gtagttcatc ttcactgatg gtttcgtggc gggttaaggt acttcccacg
     1321 cacaccagac ctgcggcgac atccagcgcc gacgcaatga ccccgccgtg taaaatgctt
     1381 tgcgcccagt tacccaccat catcggctga tttttaaagg ccagctgtgc gaactctttt
     1441 tcgtaacgct ccagttccat ccccaatgcg cggttaaatg gcatgtgata aacaaacatc
     1501 tcacccacta atttcagggc ttgttcagcg gtcagtacgg cagacataac atccttacac
     1561 ttcattagtt aatgaaatgt tgattttatg cttctttatt gttggtttcc actttaggaa
     1621 gggataacta acggctatgg agttaagtat gtaaaatagc ccgcagaaaa tattcacctt
     1681 atcaataatt cgttacggag aacacgagcg atgcggactc tgcagggctg gttgttgccg
     1741 gtgtttatgt tgcctatggc agtatatgca caagaggcaa cggtgaaaga ggtgcatgac
     1801 gcgccagcgg tgcgtggcag tattatcgcc aatatgctgc aggagcatga caatccgttc
     1861 accctttatc cttatgacac caactacatc atctacaccc aaaccagcga tctgaataaa
     1921 gaagcgattg ccagttacga ctgggcggaa aacgcgcgta aggatgaagt aaagttccag
     1981 ttgagcctgg cgtttccgct gtggcgtggg attttggggc caaactctgt actgggcgcc
     2041 tcctacacac agaaatcctg gtggcagctt tctaatagcg atgagtcatc accgtttcgt
     2101 gaaactaact acgaaccgca attgttcctc ggttttgcca cggattacaa ctttgctggc
     2161 tggacgctgc gcgatgtgga aatgggctac aaccacgact caaacgggcg ttcagacccg
     2221 acttcccgca gctggaaccg cctttatacc cgcctgatgg cagaaaacgg taactggttg
     2281 gtagaggtga agccgtggta tgtggtgggg aatacggacg ataacccgga catcaccaaa
     2341 tatatgggct attaccagct taaaatcggc tatcacctcg gtgatgcggt actgagcgcg
     2401 aaagggcagt acaactggaa caccggttac ggtggtgcgg agttaggctt aagttacccg
     2461 atcaccaaac atgtgcgcct ttatactcag gtttacagtg gttacggcga atcgctcatc
     2521 gactataact tcaaccagac ccgcgttggt gtgggagtta tgctaaacga cctgttttga
     2581 tgaatggttg gcaaatctgg aatccagcat ccaggattac cctctcagag actaaaagca
     2641 ttgcagtttc tcgcgcaggc gctgaaaata gcgcctgttt ttatttcagg caatcggggt
     2701 taatgtggcg caggcggaag tgttgaatct ggagtccggg gctaaacagg ttttacaaga
     2761 aacctttggt taccaacagt ttcgccccgg ccaggaagag attatcgaca ccgtactttc
     2821 cggtcgcgat agcctggtcg tcatgcccac tggtggcgga aaatcccttt gctatcaaat
     2881 ccctgcctta ttgctaaacg gccttaccgt ggttgtctca ccgctgattt cgttgatgaa
     2941 agatcaggta gatcaactgc aagccaacgg cgtggcggcg gcgtgcctta actcgacgca
     3001 aacccgcgag cagcaacttg aagtgatgac aggctgtcgc accgggcaaa tcagcctgct
     3061 ttatatcgcg ccggaacgcc tgatgctgga taactttctt gagcatctgg cgcactggaa
     3121 tccggtgtta ttagccgttg atgaagcgca ctgtatctcc caatggggtc acgatttccg
     3181 cccggaatac gctgccctcg gtcagctgcg tcagcgtttt ccgacgctgc cgtttatggc
     3241 gctgactgcc acagccgatg acaccacgcg ccaggatatc gtgcgcctgc tggggctgaa
     3301 cgatccgctg attcaaatca gcagttttga ccgtccgaat attcgctaca tgctgatgga
     3361 gaaattcaaa ccgctcgatc agttgatgcg ttatgtacag gaacaacgcg gtaagtcggg
     3421 gattatttac tgtaatagtc gtgcgaaagt agaagacacc gctgcgcgcc tgcaaagcaa
     3481 gggaattagc gcggcggcct atcatgccgg gctggaaaat aatgttcgcg ctgatgtgca
     3541 ggaaaaattc cagcgcgatg acctgcaaat tgtggtggcg acggtggcgt tcggcatggg
     3601 catcaacaag cctaacgttc gcttcgtggt gcattttgat attccgcgca atatcgaatc
     3661 ctattatcag gaaactggac gcgccgggcg tgatggcctg cccgcggaag cgatgctgtt
     3721 ttacgatccg gctgatatgg cgtggctgcg ccgttgtctg gaagagaagc cgcaggggca
     3781 gttgcaggat atcgaacgtc ataaactcaa tgcgatgggc gcgtttgctg aagcgcaaac
     3841 ttgccgtcgt ttggtattgc tgaactattt tggcgaaggg cgtcaggagc cgtgcgggaa
     3901 ctgcgatatc tgcctcgatc cgccaaaaca gtacgatggt tcaaccgatg cgcagattgc
     3961 cctttccacc attggtcgtg tgaatcagcg gtttgggatg ggttatgtgg tggaagtgat
     4021 tcgcggtgct aataaccagc gtatccgcga ctatggtcat gacaaactga aagtctatgg
     4081 catgggccgt gataaaagcc atgaacattg ggtgagcgtg atccgccagc tgattcacct
     4141 cggcctggtg acgcaaaata ttgcccagca ttctgcccta caactgacag aggctgcgcg
     4201 tccggtgctg cgcggcgaat cctctttgca acttgccgtg ccgcgtatcg tggcgctcaa
     4261 accgaaagcg atgcagaaat cgttcggcgg caactatgat cgcaaactgt tcgccaaatt
     4321 acgcaaactg cgtaaatcga ttgccgatga aagcaatgtc ccgccgtacg tggtgtttaa
     4381 cgacgcaacc ttgattgaga tggcggagca gatgccgatc accgccagcg aaatgctcag
     4441 cgttaacggc gttggtatgc gcaagctgga acgctttggc aaaccgttta tggcgctgat
     4501 tcgtgcgcat gtcgatggtg acgacgaaga gtagtcagca gcataaaaaa gtgccaggat
     4561 gaagactccg taaacgtttc ccccgcgagt ctaatgtatg ttgatgttat tcctcaccgt
     4621 cgccatggtg cacattgtgg cgcttatgag ccccggtccc gatttctttt ttgtctctca
     4681 gaccgctgtt agtcgttccc gtaaagaagc gatgatgggc gtgctgggca ttacctgcgg
     4741 cgtaatggtt tgggctggga ttgcgttgct tggcctgcat ttgattatcg aaaaaatggc
     4801 ctggctgcat acgctgatta tggtgggcgg tggcctgtat ctctgctgga tgggttacca
     4861 gatgctacgc ggtgcactga aaaaagaggt tgtttctgcc cctgcgccac aagtcgaact
     4921 ggcgaaaagc gggcgcagtt ttcttaaggg cttactgacc aatctcgcga accctaaagc
     4981 gattatctac tttggttcgg tgttctcgct gtttgtcggt gataatgtcg gcaccaccga
     5041 acgctggggc attttcgcgt tgattattgt cgaaacactg gcgtggttta ccgtcgttgc
     5101 cagcctgttt gctctgccgc aaatgcgccg tggttatcaa cgtctggcga agtggattga
     5161 tggttttgcc ggggcgttat ttgccggatt tggcattcat ttgattattt cgcgttaatg
     5221 ccagacgcgc catcagagta agtcggataa ggcctttacg ccgcatccga cattattttc
     5281 acgcatgcct cgccgatgct aacagcgctc ccaccagcat aaataacgag ccgaaaatct
     5341 tattcagcgc cttcatctgc tttggtcctt taatccatag agcaatccgt tgagcaaggg
     5401 tggcgtaacc gatcatcaca ataatatcga ccacaatagt ggtgacgccg agcacgatat
     5461 actgcatcag ctgcggctgt tgcggcatga tgaattgcgg aaatagcgcc gccagaaaca
     5521 caatactttt gggattggtg agattgacaa aaactgcgcg ctggaacaaa tgtcgacgcg
     5581 attgagtaga ggccagtgat ttaaggtcaa ttgcaccagc ggcgcgccat tgctgtattc
     5641 ccagccaaat caagtaagct gcgcctgccc acttcaacac ttcaaacgca atcactgagc
     5701 gggaaaatag cgtccctaac cccacgccaa ccagcacaat atgaatcgcc agtccggtct
     5761 gaagcccagc aatagacgcc accgcgccgc gataaccgtg gttgagcgag gtggtcatgg
     5821 tgttgattgc gccagagcct ggcgacaggc ttaaaatgat cgatgtcagc aggtaggcaa
     5881 accaccattc taaggtcatg atgaactccc ggtgtgtcta tttttgtgcc acaatacgct
     5941 actgtcgcag cgttgtgtca ggcacgctta aaaaaacgat tttacgtggt ttaagaggca
     6001 gattacccga tgtttcagca gcaaaaagac tgggaaacaa gagaaaacgc gtttgctgct
     6061 tttaccatgg gaccgctgac tgatttctgg cgtcagcgtg atgaagcaga gtttactggt
     6121 gtggatgaca ttccggttcg ctttgtccgt tttcgcgcac agcaccatga ccgggtggta
     6181 gtcatctgtc cggggcgtat tgagagctac gtaaaatatg cggaactggc ctacgacctg
     6241 ttccatttgg ggtttgatgt tttaatcatc gaccatcgcg ggcagggacg ttcaggtcgc
     6301 ctgttaggcg atccacatct cgggcatgtt aatcgcttta atgattatgt tgatgatctg
     6361 gcggcattct ggcaacagga ggttcagccc ggtccgtggc gtaaacgcta tatactggca
     6421 cattcgatgg gcggtgcgat ctccacatta tttctgcaac gccatccagg tgtatgtgac
     6481 gccattgcgc taactgcgcc aatgtttggg atcgtgattc gtatgccgtc atttatggca
     6541 cggcagatcc tcaattgggc cgaagcgcat ccacgtttcc gtgatggcta tgcaataggc
     6601 accgggcgct ggcgcgcgtt gccgtttgct atcaacgtac tgacccacag cagacagcga
     6661 tatcgacgta acttacgttt ctatgctgat gacccaacga ttcgcgtcgg tgggccgacc
     6721 taccattggg tacgcgaaag tattctggct ggcgaacagg tgttagccgg tgcgggtgat
     6781 gacgccacgc caacgcttct cttacaagct gaagaggaac gcgtggtgga taaccgcatg
     6841 cacgaccgtt tttgtgaact acgcaccgtt gcgggccatc ctgtcgaagg aggacggccg
     6901 ttggtaatta aaggtgcgta ccatgagatc ctttttgaaa aggacgcaat gcgctcagtc
     6961 gcgctccacg ctatcgttga ttttttcaac aggcataact cacccagcgg aaaccgctct
     7021 acagaggttt aaatttctta tgtaccaggt tgttgcgtct gatttagatg gcacgttact
     7081 ttctcccgac catacgttat ccccttacgc caaagaaact ctgaagctgc tcaccgcgcg
     7141 cggcatcaac tttgtgtttg cgaccggtcg tcaccacgtt gatgtggggc aaattcgcga
     7201 taatctggag attaagtctt acatgattac ctccaatggc gcgcgcgttc acgatctgga
     7261 tggcaacctg atttttgctc ataacctgga tcgcgacatt gccagcgatc tgtttggcgt
     7321 agtcaacgac aatccggaca tcattactaa cgtttatcgc gacgacgaat ggtttatgaa
     7381 tcgccatcgc ccggaagaga tgcgcttttt caaagaagcg gtgttcaatt atgcgctgta
     7441 tgagccggga ttgctggagc cggaaggcgt cagcaaagtg ttcttcacct gcgattccca
     7501 tgaaaaactt ctgccactgg agcaggcgat taacgctcgc tggggagatc gcgtcaatgt
     7561 cagtttctct accttaacct gcctggaagt gatggcgggc ggcgtttcaa aaggccatgc
     7621 gctggaagcg gtggcgaaga aactgggcta cagcctgcaa gattgtattg cctttggcga
     7681 tggaatgaac gatgccgaaa tgctgtcgat ggcggggaaa ggctgcatta tgggcagcgc
     7741 gcatcaacgg ctgaaagatc tgcatccaga gctggaagtg attggtacta acgccgaaga
     7801 cgcagtgccg cattatctgc gtaagctcta tttatcgtaa tcgttcttta tttggtcagt
     7861 tgtcaacctg atacttcgct acaatggata ctcgttaatc aaagagtttt ccattgtggc
     7921 gctacttatc atcaccacga ttctgtgggc cttctccttt agcttttatg gcgagtacct
     7981 tgcggggcac gtcgatagct attttgcggt gctggtgcgc gttggtctgg cggcactcgt
     8041 ttttctgccg tttctgcgca cccgtggcaa tagcctgaaa acggttggcc tgtatatgct
     8101 ggtgggcgcg atgcagctcg gtgtgatgta tatgctgagt ttccgcgcct atctctacct
     8161 gacggtctcc gaactgttgc tgttcaccgt actgacgccg ctctacatca cgctgattta
     8221 tgacatcatg agtaagcgcc gtctgcgctg gggctatgcc tttagcgcct tgctggcggt
     8281 gattggtgcc gggattattc gctatgatca ggtcaccgac catttctgga ctggcttatt
     8341 gctggtgcaa ctctccaata tcacttttgc catcggcatg gtgggttaca aacgtctgat
     8401 ggaaactcgc ccgatgccgc agcataacgc ctttgcgtgg ttctatcttg gcgcgttcct
     8461 ggtggcagtg attgcatggt tcttgctggg aaatgcgcag aaaatgccgc aaaccacgtt
     8521 gcaatggggc attctggtgt ttcttggcgt ggtggcttcc gggattggct actttatgtg
     8581 gaactacggc gcgacgcagg tggacgccgg aacgctgggc attatgaata atatgcacgt
     8641 tccggcaggg ctgctggtaa acctggctat ctggcaccaa cagccgcact ggccaacgtt
     8701 tattacaggc gcgctggtga tcctggcctc actgtgggtg catcgtaagt gggtcgctcc
     8761 gcgctcttca caaacggcag atgatcgcag gcgtgattgc gcgctgaacg aataaacgct
     8821 tctgtaatcg gctgacgctg ctcgccatcg cgcacggcgg cgtacagtcg gctccacaag
     8881 ccttcgccca gggttttggt gaccaccaga ccctggcgtt caaaactctc caccacccaa
     8941 tgaggtagtg cggcaatacc catccgtgcg gcaaccatct gaatcaacaa taaggtgtta
     9001 tcgacgcttt tcagtgacgg gctgacgcct gctggctgaa gaaaatgccg ccagacatcc
     9061 agtcggctac gctgcaccgg ataaatcaac agcgtttcgc tggcgagatc ttccggtgta
     9121 atacgcgttt ttgccgccag tggatggtca ggtgctaaca ccagacgcac ttcatagtcg
     9181 aacatcggcg aataatgcag gccactgcgc ggcagaatat cggacgtcat caccagatcc
     9241 agctcgccct gttgcaacgc aggctgcgga tcaaacgtta cgcccgattt aaaatccatc
     9301 tccacctgcg gccagttctt atggaaattt tctaatgcgg gcgtcagcca ctgaatacag
     9361 ctatggcact ctatggcaat gcgcaggcgc gtctgctgcg gttcattgca ggcttgcagt
     9421 gcctggctaa tttgcggcag cacctgattt gccagttgca acaggatttc tccctgcggt
     9481 gtaaagcgaa gcggctggct tttacgaaca aatagccgga agccaaggcg ttgttccaga
     9541 tcgctaaact ggtgagacag ggcggattgc gtttgatgca acgtcgccgc agcggctgcg
     9601 agtgagccgc agttccgcaa cgcttgtagc gttttcaggt gttttacttc gatcatgaaa
     9661 gtccttcact tcggcatgaa taatttgcgc ttgaggaata tacagtaacc gccaattatg
     9721 gatgtgtaaa catctggacg gctaaatact aaaaattcca taaggggcat ataatgacaa
     9781 ttcttaatca caccctcggt ttccctcgcg ttggcctgcg tcgcgagctg aaaaaagcgc
     9841 aagagagtta ttgggcgggg aactccacgc gtgaagaact gctggcggta gggcgtgaat
     9901 tgcgtgctcg tcactgggat caacaaaagc aagcgggtat cgacctgctg ccggtgggcg
     9961 attttgcctg gtacgatcat gtactgacca ccagtctgct gctgggtaat gttccgccac
    10021 gtcatcagaa caaagatggt tcggtagata tcgacaccct gttccgtatt ggtcgtggac
    10081 gtgcaccgac tggcgaacct gcggcggcag cggaaatgac caaatggttt aacaccaact
    10141 atcactacat ggtgccggag ttcgttaaag gtcaacagtt caaactgacc tggacgcagc
    10201 tgctggagga agtggacgag gcgctggcgc tgggccacaa ggtgaaacct gtgctgctgg
    10261 ggccgattac ctacctgtgg ctgggtaaag tgaaaggtga acagtttgat cgcctgagcc
    10321 tgctgaacga cattctgccg gtttatcagc aagtgctggc agaactggcg aaacgcggca
    10381 tcgagtgggt acagattgat gaacccgcgt tggtactgga actgccgcag gcgtggctgg
    10441 acgcatacaa acccgcttac gacgcgctcc aggggcaggt gaaactgctg ctgactacct
    10501 attttgaagg cgtaacgcca aacctcgaca cgattactgc gctgcctgtt cagggtctgc
    10561 atgtcgatct cgtacatggt aaagatgacg ttgctgaact gcacaagcgt ctgccttctg
    10621 actggctgct gtctgcgggt cttatcaatg gtcgtaacgt ctggcgcgcc gatcttaccg
    10681 agaaatatgc gcaaattaag gacattgtcg gcaaacgcga tttgtgggtg gcatcttcct
    10741 gctcgttgct gcacagcccc atcgacctga gcgtggaaac gcgtcttgat gcagaagtga
    10801 aaagctggtt tgccttcgcc ctgcaaaaat gtcatgaact ggcattgctg cgcgatgcgc
    10861 tgaacagtgg tgatacggca gctctggcag agtggagcgc tccgattcag gcgcgtcgtc
    10921 actctactcg tgtacataat ccggcagtag aaaagcgtct ggcggcgatc accgctcagg
    10981 acagtcagcg tgcgaatgtc tatgaagtgc gtgctgaagc ccagcgtgcg cgttttaaac
    11041 tgcccgcgtg gccgaccacc acgattggtt ccttcccgca aaccacggag attcgtaccc
    11101 tgcgtctgga ttttaaaaag ggtaatctcg acgccaataa ctaccgcacg ggcattgcgg
    11161 aacatatcaa gcaggccatt gttgagcagg aacgtttggg actggatgtg ctggtacatg
    11221 gcgaggccga gcgtaatgac atggtggaat actttggcga gcatctggat ggctttgtct
    11281 ttacgcaaaa cggttgggta cagagctacg gttcccgctg cgtgaagcca ccgattgtta
    11341 ttggtgacgt tagccgcccg gcaccgatta ctgtggagtg ggcaaaatat gcgcaatccc
    11401 tgactgataa accggtgaaa gggatgttga ccggcccggt gactattctc tgctggtcgt
    11461 tcccgcgtga agatgtcagc cgtgaaacca tcgccaaaca aattgcgctg gcgctgcgtg
    11521 atgaagtcgc ggacctggaa gccgctggaa ttggcatcat tcagattgac gaaccggcat
    11581 tgcgcgaagg tttaccactg cgtcgcagcg actgggatgc ctatctccag tggggcgtgg
    11641 aggctttccg tatcaacgcc gccgtggcga aagatgacac acaaatccac actcacatgt
    11701 gttactgcga gttcaacgac atcatggatt cgattgcggc gttggacgca gacgtcatca
    11761 ccatcgaaac ctcgcgttcc gacatggagt tgctggagtc gtttgaagag tttgattatc
    11821 caaatgaaat cggtcctggc gtctatgaca ttcactcgcc aaatgtaccg agcgtggaat
    11881 ggattgaagc cttgctgaag aaagcggcaa aacgcattcc ggcagagcgt ctgtgggtca
    11941 acccggactg tggcctgaaa acgcgcggct ggccagaaac ccgcgcggca ctggcgaaca
    12001 tggtgcaggc ggcgcagaat ttgcgtcggg ggtaaaatcc aaaccgggtg gtaataccac
    12061 ccggtctttt ctcattacag cgacgtcttc ccagcatact gcgtaaacca ttccagatat
    12121 cagttcatac cctgtttaag aaaatcttct ttattccctt ctcatgaaat aaatttcgtc
    12181 atttccgcca ttttttctat ctgcctctcc attacatatt aaaaatcact tgatatctct
    12241 atgtttttta atgaaaaaca tatcaaccta tgatctttct tgtgactagc ttcacatttc
    12301 atactgatgc attggaatga aataaaattt catgagattt tgtgtttgag aaaaattatt
    12361 tcatgaggtg tgaaatggac gcaaagctgt acatcactga gatcatcaat gaattaacct
    12421 tcgttaagga ctctatccgt gaggatgact gcaccaaatt gatcgacgct attcagcaaa
    12481 gccagcgcgt tttctgctat ggcctgggac gtgccggctt ttccatgaaa gccttcacca
    12541 tgcgcctgat gcatatggga aaagaggttt acttcctgac ggaaaccatt acgccgaact
    12601 ttgggcctgg cgatctgttt atcgtttctt ccgcttccgg tgagacagca caactggtgg
    12661 cactggcgaa aaaagcacgt cagcttggcg gcgctgtggc ggtgttaacc acgaatcgac
    12721 acgccactat caccgagttt gtcgatgtca tcgtgcaaat taatgcccct tccaaaaatc
    12781 aaaaagattc tgttttccgc tcggcacagc cgatggcgtc gttgtatgag caagctttgc
    12841 tggtgattgc cgatgcatta gtgatgaaga tggctgctga aagtggtgcg ccggaaagtg
    12901 aactgtttaa gcgccacgcc aatctggaat agtcgtcgcc gttatttgct cgatacccta
    12961 caggagaata aaaatgagca aaaaaataac atccttgttt aacttcaaga agttgcagaa
    13021 attttcttct gctctgatgt taccaatcgc cattcttccg gcggcgggtt tgattttagc
    13081 catcgggatt tcatttaaca ttgagctgct ggcgaattgc ggcggcctga ttttcgccaa
    13141 ccttcctttg ctgttttgtg tcggtattgt gattggcctg acaggtgatg gttcttcatc
    13201 actttcagcg attatcagtt acatgatcat gaatgccacg atgggaacgc aacttggcat
    13261 taccgcggag tccgttgcag ccagtaacgg tgactatgcg atgatcctcg gggtaccgac
    13321 gttacaaacg ggagtgtttg gcggtttgat tgtcgccatc tttgtttatc tgctctatac
    13381 ccgcttccac aatattgaat tgcctcagtt tcttggtttc ttcggtggaa aacgctttgt
    13441 acctattatt acggcggtgg cggcaattat tgtcggctat ttattgccgt ggctgtggat
    13501 accggtacaa aaaggtctgg cgtcgctttc aacgctggtg accagtgggc ataacgcgaa
    13561 cttcgcggcc tttatttacg ccgttggcga acgttcgctg attccttttg gcctgcatca
    13621 tatctggaac gtgccgtttt attacaactt tggcgactac atgaccaaat ccgggcaact
    13681 ggtgacgggc gatattccgg tgttctttgc ccaattgcgc gacggtgttc cgttgacggc
    13741 ggggctgttt atgacaggtc gtttcccgat catgatgttt gcgttacctg ccgccgcgtt
    13801 ggcgatgtat caggaagcaa aaccggaacg taaggcgctg attaaaggcc ttttactctc
    13861 cggtgcgctg accacatttg ttaccgggat tacggagccg ctggaatatg catttttgtt
    13921 tgcagctccg gttctctaca tcattcacat cttcttatat gccacttcat ttgtgctgat
    13981 gaacatcctg aatgtgcaca ttggtcatcc ttttgctggc ggtcttattg atttcgtact
    14041 gaatggcgtt atgccaaacc gcacgccgtg gtatctggtg ttcctggcag gcgcgggcta
    14101 tgcagcgatt tactacaccg tatttcgggt actgatccgc aagttgaatc tgaaaacacc
    14161 ggggcgtgag gatgaagaga ttgccgttgc aaccacatta acgcgcgaag aacgtccata
    14221 ccaaatcatc gaagcagtag gtggtttcga caatatcgaa gatgtggatg cctgcgcaac
    14281 acgcctgcgg ctggcgctgg tagacgataa gaaagtgaat gaaaaacggc tgaaggagtt
    14341 gggggctgcg ggtttagtga aactgggtga tggcggtgta caggtgatat ttggcggcaa
    14401 atcgcaaatc ctgcgcgacg aaatcaaaac cgtaatgagc agaccgcgcc cgacagaaat
    14461 gacagtggcc tgtgctaact aattcgagat tagcgggctg gtttcagccc gccagacaga
    14521 gagagctgaa gtatgaattc acaattactg gctaacgcca tccggatgtt aagtgttgat
    14581 gcgatccaga aagcgaactc cggtcatccg ggcgcgccga tggggatggc ggatattgcc
    14641 gaagtggtct ggcgtcgtca cctgcgtcat aacccaaaaa atccgcagtg gtttaaccgc
    14701 gaccgctatg ttcagtcgaa cgggcacggt tccatgttga tttacgcttt gctgcatctt
    14761 acaggctacg acctgtcgat ggatgatatt cgcgactttc gtcagctcca ctcccgcacg
    14821 ccaggtcatc cagaatatgg ttatacgccg ggtgttgaaa ccaccaccgg cccgttgggg
    14881 cagggcgtag ctaatgcggt ggggatggcg attgcggaga aggcgctggc ggcagaattt
    14941 aacaaaccgg gctttaatat tgttgatcat cacacatggc tatttttggg tgatggctgc
    15001 ctgatggaag ggatctccca cgaggcctgt ggtcttgcag gaacgctgaa gctcggcaat
    15061 ctgattgcta tctgggacga taacggtatt tcgatcgatg gtcacgtaga aggctggttc
    15121 gctgaagata cggctgcccg ttttcgtgcc tacggctggc atgtcattga aggcgttgat
    15181 ggtcacgatc cagaagcggt agatgctgcg gtgcgtgaag cgaaaagcgt cacggataag
    15241 cccagtctgc tgtgctgcaa aacgattatc ggttttggtt cgccaaataa agccaacagc
    15301 cacgattgcc acggctcggc gctgggtgca gatgaagtgg ctctggtgcg tgaacgtctg
    15361 caatggccgt atgctccttt cgaaattcct ggtgaaattt atgccgcatg ggatgcgacc
    15421 gagaaaggcg cgcaggttca acaggaatgg gatgcactgt ttgctgacta cgcgaagcag
    15481 tggccggaac tggcggcgga gtttactcgt cgcatgaagg gtgatttgcc tgctggctgg
    15541 gccgagaata tgcagaaata tgtgcacgat ctgcaatccc atccggcggc gctggcaacc
    15601 cgtcaggtca gccagaaatg cctcaatcac ttcgcggata tgctgccgga actgatgggc
    15661 ggctcggcgg acttgtcgcc ctctaacctg actcgccatc agaaatcggt tgatttcacc
    15721 ggggaaaatc cggcggggaa ttacatctcc tatggcgtgc gtgaatttgg tatgtcggcc
    15781 atcatgaacg gtcttgcgct gcatggtggg tttatccctt acggcggtac tttcctgatg
    15841 tttatggaat atgcgcgtaa tgcactgcgc atggcggcgc tgatgaagat ccgctcggtg
    15901 tttgtttata cccacgatac catcggcctg ggcgaagacg gcccgacaca tcagcccgtt
    15961 gagcaactgg cgtcgctgcg tctgacgccg aatatggaaa cctggcgcgg ttgcgatcag
    16021 gtggaagtgg cggtggcctg gcaacaggcg attgagcgca aagatggccc aacgtcgttg
    16081 gtattaacgc gccaacctct ggcgcaacag ccgcgcaccg ctgcacagtt ggcagagatt
    16141 gcgcgtggtg gttatgtact gagtgattgt gacggtcagc ctgagatgat cctcatttct
    16201 gcgggttcag agattgagtt agtggtcagc gccgcaaaag cgttaacgga agaagggcgt
    16261 aaagttcgcg tggtttccct gccgtgtacc gagcgtttcg ataatcagga tgcggcgtac
    16321 aaagagtcgg tattgccaaa agctgtacgc aaacgtctgg cggtagaagc cagcatcgcc
    16381 ggattctggg aacgctacgt gggcctggac ggtaaagtga tcggcatgac gagctttggt
    16441 gagtctgctc cggcgaatgt gttgttcaaa cactttggtt ttacgccgga aaatgtcctg
    16501 gcgcaggcgc gtgagctact gaacagctaa acctgcacaa ggggtgaaag gatgaccttt
    16561 taccccttga aacgggagcc aagaaactct tcacgggtgc ggtatttctc gtaatgcgcc
    16621 ttgtttttat acataaacaa cagcaccagc atatcaataa cgcccattgc cggaacgcga
    16681 gtggagtcga aggcttcaaa gaagatttta tcgctgaccg cggtattgag ttgatgggtg
    16741 gagagttttg ataacggaga tttaatatag ttagtgatac aaacggattt ggcaccgcgc
    16801 tctttcacca ctttaaagat atcgaccatt aagttggtgc tgccagagtt agaaatacca
    16861 atcgccaggt cgcgtttccc catagaatat gccgcattaa tttgcatttc cgggctgacg
    16921 tgaacaacgg tttttaatcc gagttggctg aatttataag caccataaac ggccagaggg
    16981 tttgaatttc cggcagcaaa aaagaaaata gtatcgcaat tagcaagtag tttgagaatg
    17041 gactcaagca cttcggcatt aaaaaattgc gttgattgta ttaaatcttc ggcctgtttt
    17101 tttaataaaa atgtggccag cgacgttata tcggtaaaat cggtattatc aggatagcta
    17161 ttgtcaggat taatgacttc ttttgctaat gcaattttta aatggtaata accctgataa
    17221 cctaatttct tacaggtgcg tacgatagtc gcttcactgg catggctttt ttctgatagt
    17281 tcagtgacac tatagttcac gacttcatgt gcgtggctaa gaatatagtc agcgaccttt
    17341 ttttccgcct gatgcagtgt agggtaattg ctttgaatca gactttttac ggttatgtta
    17401 ttcataatta gtaaatttca ttctgacaga aagtgctgat tataacatag ttttcaggcg
    17461 tggataggtg tgaaaaaatg aatgtgtgca gtgaacgtaa tgattaacag accggttaat
    17521 tttcatgaat tacgtcaact gcacaaaaat aatttaaacg ggtgctctaa ttaaagcaag
    17581 acttatattt ttatcattgt attgtcaatt aaatacaaat cgtgtcatta attgtgggtt
    17641 tcttaaatat tctatgcacc actgacaggc tttgcccata ccatcggaac gagcggcaag
    17701 aaataacttg gtggcgtgtt tatgttcgcg cattggcttt ttaagcaatt taccgctgtt
    17761 caataacggc aatgccagat gatgtggaat ataaccaata ccgacgtttt gttctattaa
    17821 ggcgattgcc gtagcgaagt caggaacaaa aatgggtttt tgtccttcca acaaccaggc
    17881 ttgcatcggg gggaagttaa cggaggtatc gcggatacaa accgctggat aatgacgcaa
    17941 ttcactgttt tctaatggat gccgcttcgc tgccagtggg tggagtgggc tgacgacaaa
    18001 atcccattcc atttgtccga ccggttcgct aatgatgcct tcactgcttg gtactgcatg
    18061 aggagcaccg tagaccagat cagcacgctt actgtacagc gcatcccagc agccgttgta
    18121 tacctcaaga tctaccgtca gtgttgttga tgtgaattgc cgttcaaaat ctcgaataaa
    18181 tgccaccagc aaatcaccag gaataatatt gtttaccgca attgtcagtt cacgctcaat
    18241 accatcgtga atcagcactg tgttgcgctt taatgcttcc aggtcgccaa gaatagtttt
    18301 gctatgttcg atgaagtaac ttcccgctgg cgataattca atatagcgtc ccttacgaat
    18361 gaacagttca acgcccaggc tctcttccgc tttcttgatg gtgtaactga tggcggaagg
    18421 gactttattt aactgctcgg ctgcggtggt aatgctctgg tgtctggcga ccagatgaat
    18481 aacacgaagc gtttcgtctg agatgaacat aaggctccct ggtcagtggt aaagtggctg
    18541 aatctctcca gattcagccg taatcattgc ctgagtatac ccaggaatta gcggtgttga
    18601 cttgcccgac tgagaatcgc atacgcgata catgccacca gaatcgagtc taccgaaggg
    18661 atcgtcgtca gggtgaataa gccttttacg gtcataaagc caaccgctgc gcctatgatc
    18721 catgctgcga atgtcagcac tttaatctgt gactcctggg ctaacactga ctcttgatag
    18781 ccattacggc gataaaggaa aaagtcagcg atataaattc cggcaacggg tggcgtggcg
    18841 atacccagga acagcagaaa cggaataaac cacgccataa tatccatact gcctacgatg
    18901 gcggaaagga tacccagcgc cacggtaatc tgccatttgg gaaagcgggt aagcagtgtg
    18961 gaaaccacca gcgtgccctg gaacatattg cccgcgttac cggtgacgca ggcgaaaatc
    19021 aacaacagcg ccgcaggcag aaccgcaccg aataccgcca tcgcacccag taaagatccc
    19081 tgaccactaa tggcgctcgg cgttgcaccc gcccagtaga gcagagggta ggcagccaga
    19141 aacgtcacgc ctgcggcaat cagcgcatgt ttgcggttat gcacaaaact accgaaatca
    19201 ggcagtgtgg caaccagcac aataatggtg ccaacaacgg tggaaacggc gacacctgta
    19261 ttcatctcca caatcgccgc tggtactgcc acttcggtat gggttgccac atagaggatg
    19321 taacacagca ccagcgcgat aaccggcacg gcaacctggg caatttttcc cagaacggca
    19381 aagccaaatg ccgtagaggc gacaaaaatg acacagccag ccgccactaa cagtgccagt
    19441 ggaacttcca gtccatgttg agccagtaag tcatgtaccg aatgaccaaa catattggcg
    19501 gtaacggcaa tccagccgaa cagacttatc gccatcaaaa tgttaatggc gatggcccct
    19561 ttttcgccaa acgaatattt cacaatgcca tacgttggca ggcgggcttt ttctcccacg
    19621 ctaatggtga tggctgaaag taaggtcaga atgacgccgc ctaacaatac gacgctaatt
    19681 aaactgctgc tggaaagttg attgccgagg cttgaggatg acaacagtac aggtgtgacg
    19741 gcaataccca gcaaaatcat ggcaatacgc aatcctgatg ccgtattcag attctcttct
    19801 tttttacgca tagtatgttc cctttataaa tgcgcgttac acagtatgaa aatgcccggt
    19861 gtatttgcgc cagctaccca gttctgtaat ttcccgttgg ccttcggtca gccagttttc
    19921 tgccagtacg cccagcactt cgctaccatc ggcaagtttg attttgccaa ttgccagccc
    19981 agcgggttcg ctcattaaca gagcggcaaa actggccagc ggtaattgcc aaatttctac
    20041 gtcgacgtgt gttcctcctt cattcacgcg aatcattccc ggatggcgat cattaatact
    20101 ccacaaccgg taatgggcgt cagttcggtc ttcacggaca aaaataccgc ccgctttttg
    20161 catatttggg ttgagttcca ggccgcgcat cagcgttccg ttaacggcta aaagcgttgt
    20221 agtcattgtg ttctccagag aattaaaggg ttatccatgt accgctctga tgcgccaggg
    20281 ctgcttttat ggtttgcggt gaagtaatca ggccgctggc ctgtttgcct tgttgctggc
    20341 tggcattgat gaaatcaaca atggcttcca ctttgggttg catactgccg acaccaaact
    20401 gaccttcccg caacagcgtt tgcgcttcgg caactctgat agcgtcgagc cactgttgtt
    20461 gtggtgtacc aaagttaatc gccatttttt ctacaccggt ggggatcacc agcagatctg
    20521 cgcctaactg actggcgagc agcgcggagg ccagatcttt atcgatcacg gcttctacac
    20581 ctttcagttg ttgcccgtct cgcaccaccg gaataccgcc gccgccgcag gcaataacca
    20641 gatatccctg gcgcaccagg tgagcgatgg tgtcgtgctc aataatttcc agtggtgcag
    20701 gagagggaac tgtacgccgc caaccacgcc cggcgtcctc catcagcgtc cagccgagtt
    20761 gttgttggcg ttgttgggcc gttgcttcat cgagaaacgc gccaattggt ttactggggc
    20821 tggcgaaagc atcgtcatgt gggctgactc gcgtttgtgt caccagggca attaccggtt
    20881 tgtttatgcc acggcgagcc aattcgttat gcagcgcttt ctggaacatg tagccaattg
    20941 caccttgtgt atcacccacg gcgtaatcaa gtggaaccgg agaaacttcg ttactggcga
    21001 gttcagagcg gcgcagaata aagcccacct gcgggccatt accgtgggtt agcaccaggt
    21061 cccatccggc ttcaaccata tcaacgatat gctgcacgct ttccatcact gcaacatatt
    21121 gatcgggaat actattgcgt tgctcgtcct gaatgagcgc attgccgcca accgcgacga
    21181 cagccagtgg cttaaccatg atggttctcc tttgcgacga tctccgccag tgcacgtaca
    21241 gcggcgacaa aacaggccat cggcgcagtg gtgatcccgg caccaatttg cccgaccccc
    21301 gcttgtttgt gggcgatacc cgtattgatg accggtaaaa tgccgcgatc ggtcacttta
    21361 cgcgcatcaa tccctgctgc ggtgggggca aaattgaggg ccggaagtgt aaatgcgggg
    21421 ttaccgccga gggtgatggc ttgcatccga cggctgttat tggtggcatc gacaggggtg
    21481 cctcccacaa atttgacgat cgccggagac gacgccatcg cgaagccacc cacacctgcg
    21541 gtttcagtaa ttgcgctatc acccaggtca gcagcagcgt cgtcaacgcc aaatccgggg
    21601 aagaacagac cttcaacggc attggcagga gcctggaacc agcgatcgcc agtaccggaa
    21661 atccgaatac caaaattcac cccgttacgc gccatcaccg taaccattga actgaacggt
    21721 acattggcgg cggcatccat cattgcttta caggcggcca tcgacagatt gaggaagaag
    21781 tgatcgttac cggtgataaa tgccacgacg cgctggatat gttcctgtgg aagagagcag
    21841 gccagcaatg ccgggagcag gcgcttaatc agcaaaccgg tagcggcagc attgcggtta
    21901 tgaacttcat cccccatatg cagcgcctgc gccattaatg gcttgagttc cagctcgccg
    21961 tgttgtgcga ttgctgcttt cattgccggc gccagttcat cgcgcatcca ggccagacga
    22021 ttgagaactt catcgttatt ggcgccaaaa cggagaacct tacccagacc ttcattgaaa
    22081 ttgctaaaag tacgatggcc gttggttttg ttttctacca cccataacgg catcgacggg
    22141 ctgatgatgc ctgccattgg gccaaccgcg tgataatgat gacaaggggc aaggtcgatt
    22201 tcgcctgcgt ttatttggtt ttcagcgtct tgctggctgg tggcccaacc ttcgtacagc
    22261 atggcaccaa taatcgcccc ttttaccgga ccacacatct cttcccaggc aatcggcggg
    22321 ccagagtgca aaatacgttt ttgttcagcc attgctggaa tggcctcttt agcgagcatg
    22381 acatccacca gcatgggctg tgcttcgaga taacgtgtca tggcgatttg gttggcattt
    22441 tctaccaggg gatgacgtag caacgatgcc agatccaatc cagcgtcaat atcgccttgt
    22501 gctggtggct gccagttgag tgcaattgcg cttcccccgg catgttgaat attgtcagca
    22561 aaactgtgta gtcccgcgtt cacaaccttc aaaggctgat taaataaggt attcatgcgt
    22621 ttagcccctt tttctgcaac tgagtctgcg caacggcgct ggcccatagc gccgcctgtg
    22681 cattgctgct ggcaatgatg atgcccgcat tttgtagtgc tcttatctgc tgactgcgta
    22741 tttgcggatc agcttccgtt ccgcaaacgt gcgcaatcag caacggagcg ttattcatgt
    22801 cgatgtgtga aagttggtcc agtaattcgc tggcaggcgt cgtggaagcg ccataaccaa
    22861 gtaccaggtc gaataacacg acggcagtgt gtgagtcgtt gagttcgttg agcagacgtt
    22921 gattgcgcag agtcgggtcg atcattggat gcggtttgcc acgggtgaaa tcatcgtcgc
    22981 ccatatcgat caacgtgtga tcttcgcttt gccagatatt tgccagagca cgattacccg
    23041 caactggcgt attggaagct gcgctgaatc ccttttgttg gcagataagt tgcgcttcgt
    23101 agcagaaggt tcctccggcg aaaacaccgc gtatcgcctg gcgatgggct ggcaggcgct
    23161 gacaggcgtt ttgtaacatt gtgagatcgt cgcagggtac atcggtttcg acagacggca
    23221 gtggctgagc attcagtaac gccacagcga tgttggcggc gctggcaagt gtggtggccg
    23281 cggtgatatt gggacgtgcc agatcatgcg gattcgcacc gaggaaattc accaccacgg
    23341 gttttccaca agcctcggca cgctccagaa tagtccgggc aacgatcggt gaaggcggtt
    23401 ttgaaataag cacaatcacg cgagtttcat cgtcttgcgc cagcgcatct aaagcgaaga
    23461 gcatcgatat tccaccaatc tcttcactga gatcatgtcc gcctgttccc aacgcctgag
    23521 aaatacccgc tccgagctgg tcgattcggc aagtcacttc ctgtagcccg gtgcctgacg
    23581 cgccaataac accaattgct ccgcgtttca ccacattagc gaagcctaac ggaatgccgt
    23641 taacaatggc ggttccacag tcaggtccca ttacgatgcg ctgacgttct cgggcaagcg
    23701 ttttgatgga tttttcctgc ccgatactga cgttatcgct gaacatcatc acgttcattc
    23761 cgaggtttaa cgctttaatt gcttcggcgg cggcgtagtc tcccggtaca gaaatgagtg
    23821 ccaggttggc ttcgggttcg gcttccagtg ccatttccag gctgaccttt tccggtgttt
    23881 taatgccact atccgtttca tcatcgggtt tgctggtcag acgttgctgg gccagtacca
    23941 gtgcttcatt gcaaatatct tcttcaccca tgactgcaat cactaaatca ttgggactgg
    24001 cgttgatttc gttacccaga cccgcatcgc gcaattgttc gagattattg ggcgttccca
    24061 tgacgaccga ggcctgttga ataccgggca acttgctgat ttgcgcggaa atctgcatca
    24121 gagaaactga gtcctgataa agattactga aaactttata tttaactttc atgctgttac
    24181 gcctgtttca ttccgcccga tagcaaaatt cgggcaaagc atccccgtgt agccccataa
    24241 acgggctacg ggagaaaagg ttgaaatcaa ttcatggtgt tcagggttcg aaagccatgc
    24301 agcattcctg caaggcaatc tactcctgac gttgcgccaa attgcatgac ctgttctgca
    24361 caggatgcga ttgtcattgc cgatgcgctc ccaaccagtt gtgcgagtaa ctggcaaatc
    24421 ggttctgaaa aatgtccctg aagcgcacgc tccagataat gacgactgat atccgtggtg
    24481 ttgtgcgcat gttgatcaat tgcctgctgc aaccggtatt gatgatcggc aagtggtgca
    24541 gggagttgcc ataaccagag tgctgccaga tagcccaaca aatagtcatc tccatcgggc
    24601 gttaagcctt taccaaagcc gatcagttgt ggcacctggc gttccagcgc atctggatgt
    24661 tccataatcg ctaatccctg gtgcgtgaga ctatccggca ggtgcaatgt gctttgaacg
    24721 ttatgctcca gacaaaataa aagccgttgc gatgccatcg ttgggtagtg ctggaaaacc
    24781 aaagggaatg cctcaagtgt cagcattctc cccacaaatg gcgactgcca gagcgttgcg
    24841 ttatcgagcg ccacacaaaa tcgaggtgtc cgcagtaagc cttgggttaa ctgaatggga
    24901 tctgaatggg caatctctct tcgccagtcc caacattcag gtagccagac tctggcagca
    24961 tcagggagat tcagatacgt ttgcgcacac aacagagtga tgaatgttcc cgcgtctgaa
    25021 tacagattta ccgcatgttt aaaagcgcta tgcagacgta aatcttgcgt ttcattctgc
    25081 gggaaaagat atcccaacga cgatgcgcaa agcgtaacct gtaccatcgg cgttagcctt
    25141 ccagacctgc aataatggct ttggaatcag taacccaacc gaaaatggca ccttgtgctt
    25201 tgatcatctc gagggcgtat ttctggaatt ccgggaaata ggagccgaca caatcttccg
    25261 ggataatgca ttcatagcca cgatcatttg cttcgcgcac ggtggtggtt acgcaaactt
    25321 cggttgttac accacaaact atcagcgtct tgataccgtg gttttgcaaa atcagatgta
    25381 agtcggtttg ataaaacgcg cctttgccag gcttatcaat caccggttcg cctgccaccg
    25441 ggtacagttc aggaatgatg tcatgacctg cttcacctct gaccagaatg cgtcccatcg
    25501 gtcctggttc accaataaag gtttttccgc cgcgcgtcag tttggctggc gggcagtcgc
    25561 ttaagtctgc acgatggcct tcacgagtat gaataaccat aattcctttt tgacgtgcgg
    25621 ctgccagcac ctctgtacat ggtgcgatag ccgtgcgtac cagtgaaaca tcattgccca
    25681 gtgcctcacc gaagccccct gcttcgacaa agtcacgctg catgtcgatc atgaccagtg
    25741 cagttgtgcg cggatcaaaa ggtaattcaa agggttgagc ctgaaaaatg gattgcgtca
    25801 tggtaaatct cctgtctgat gtttaaacga tccagttgtt ctgagaacga gtattcagga
    25861 gaaaagcggc acaaaacagg aaaaaagttt gccgcacagc aacgaaattt ttgcattcag
    25921 caggtggtgt gatgacgaat gtgaagggag tcaaagagtg gcatacagca acaaattttt
    25981 tgatgctgta tggttttgag attaaaaaaa tcgttgaaat tgtgtgagct taatcatcag
    26041 atttagcgac tatggcgtta ctttttaaca ccatactgtg taaaccattc caacattcgc
    26101 tgccagccgt ctttcgcaga ttcggcatga tagctcgggc gataatcggc gttgaacgca
    26161 tgccctgcat ccgggtacac gataatctct gctttcgcgt ttgctgcccg cagcgcctgg
    26221 cgcatcgttt ccacgctctc ttgcggaatg ctgttatcct ggccgccata taagccgaga
    26281 accggcgcgt taagatcggt tgcgatatca acaggttgtt tcggtgaatt cagcgactta
    26341 tcgcccgtca gtttgccgta ccacgccact gcggctttta gctgtggatt atgcgcggca
    26401 tacagccagg tgatacgtcc accccagcag aatccggtga tcattaaacg atgaacatcg
    26461 ccgccgttgc gggaagccca actggcgaca tgatcgagat cggccagcac ctgcgagtca
    26521 ggcacttttg ctaccagacc gctaagcaac gtggggatat cggcaaaatc attcggatcg
    26581 ccttcgcgga agtaaagttc aggtgcgata gccagatacc cctccagcgc cagacggcga
    26641 caaatgtcgc ggatatgttc atgcacgcca aaaatttcct gcactacaat gaccactggc
    26701 agtgggccat cgctttgctt tggtctggca tggtaagcag gcatgttatc cccttgtgaa
    26761 gggatagagg tgaagcccgc cacaatcgcg tcgtccggag tctgaacgat ggtcgaagcg
    26821 agaggcgatg cagcaggtgc aaatccagat tgttgtgttg ttgccatggt attctccgta
    26881 cccttataaa aatgttgcgc aatgttaact atagtcagca tgcaacaaat cacattgcct
    26941 gaatcggctc atcttttatg cagtcctgca gaatgaagag tgatttatgt gatttgcatc
    27001 acttttggtg ggtaaattta tgcaacgcat tttcgtcatg gtgatgagta tcacgaaaaa
    27061 atgttaaacc cttcggtaaa gtgtcttttt gcttcttctg actaaaccga ttcacagagg
    27121 agttgtatat gtccaagtct gatgtttttc atctcggcct cactaaaaac gatttacaag
    27181 gggctacgct tgccatcgtc cctggcgacc cggatcgtgt ggaaaagatc gccgcgctga
    27241 tggataagcc ggttaagctg gcatctcacc gcgaattcac cacctggcgt gcagagctgg
    27301 atggtaaacc tgttatcgtc tgctctaccg gtatcggcgg cccgtctacc tctattgctg
    27361 ttgaagagct ggcacagctg ggcattcgca ccttcctgcg tatcggtaca acgggcgcta
    27421 ttcagccgca tattaatgtg ggtgatgtcc tggttaccac ggcgtctgtt cgtctggatg
    27481 gcgcgagcct gcacttcgca ccgctggagt tcccggctgt cgctgatttc gaatgtacga
    27541 ctgcgctggt tgaagctgcg aaatccattg gcgcgacgac tcacgttggc gtgacagctt
    27601 cttctgatac cttctaccca ggtcaggaac gttacgatac ttactctggt cgcgtagttc
    27661 gtcactttaa aggttctatg gaagagtggc aggcgatggg cgtaatgaac tatgaaatgg
    27721 aatctgcaac cctgctgacc atgtgtgcaa gtcagggcct gcgtgccggt atggtagcgg
    27781 gggttatcgt taaccgcacc cagcaagaga tcccgaatgc tgagacgatg aaacaaaccg
    27841 agagccatgc ggtgaaaatc gtggtggaag cggcgcgtcg tctgctgtaa taaaaaaacg
    27901 ctccccaact tcggggagcg atatcagtta ttgacgccgt aaacgcatta ttttgcggat
    27961 tattggcgtc agtagcgtaa aggcggccag agtcagcaat gccagagtga tcggtcgggt
    28021 atacagaaag tcgagtgaac cccctgaaag cgacagcgca cggcgaaaat tactttccgc
    28081 cattggcccc ataatcaatg ccaggaccac tggcgacgcc ggatatcccc ctttctgcat
    28141 gaaatagccc actacgcccg caatcaacat aatgccgaca tcaaacaggc tgttgttcag
    28201 tgcataagtc ccgaccacac aaagtgcgag gatcatcggt gtgagaatat tgccgggaat
    28261 tttcaccaca ttaccgatga ctttcaaaga gagcagtcca agaactagta gcatgaagta
    28321 acagaagatc atgccgacaa acagcgtata aaccatatca ccgtggtcgg taaacatcag
    28381 cgggcctggt tgcattccct gtaacgttaa cgcgcccagc atgatggcgg ttaccgcatc
    28441 gccagggatc cccagcgtca gcataggtaa caatgcgcca ccggtgcagc cattcgcgcc
    28501 tgcctcacag gaggccacag ctttaatttc acccttacca aagttttctg gtgtcttact
    28561 gaaacgacga gtttcgttat aggcaacaaa agctgcaata tccgcccccg caccggggat
    28621 catgccgata aaaaccccta atcctgatga gcgcaaaatg gttaatgcga tgcggcgtag
    28681 tgtttgccag ggcagtaaca gactgccgat agccaccttc gcgccccgac gaatttgctg
    28741 gttttgctcc atagagcgga atgcttctga agcagcaaat aaaccaatca tcaccgggat
    28801 aaatggcaca ttcatcagct ccacaaatcc acctgtaaac cgtggaaaac cgcccattgg
    28861 atcaaggcct atcgttgcga tcagcaatcc gccaaccccg gcaatcagcc ccttaatcag
    28921 cgactcaccg gaaatactgg caataatgct cagtccgaag gttgccaggg cgaaggactc
    28981 cgaggcacta aatttcagcg cccaactggc aagtaccgga gagagaaaca tcagcacgat
    29041 aatgctgagc gttccgccac taaaggaaga gaacgtggcg gcactcaatg ccagccctgc
    29101 ttttccctgt ttcgtcagct cataaccatc aatagcggtg gctgcggcgg caggcgttcc
    29161 gggaattttg agcaaaattg cggtgataga accaccgtag acgccaccaa aaaagacgcc
    29221 gcaaatcatc aacaagccag aaacaggctc catgccatag gtgaaaggca gcaaaatcgc
    29281 cacacccatg gtcgctgtca gaccaggcaa agcgccaatc acaataccca gcatcacccc
    29341 aaaagtggca aacagcagcg cctgaggatt attgagtaat gtcgaaaaac cctgagttaa
    29401 aagttcactt tcaaacattg ctggcccctg ttattcgaaa aatgtgccga caggcaatgg
    29461 cacctgtaac gcttcgacaa acagcaacca gaggattgcc gttaacagca gcgcaatacc
    29521 cggtgtgagt acccatcgcc tgtagcccat caacgtcata atgacgatga ggaaaacagg
    29581 agtagtaatg agataaccgc agtagctcat cgccacaata taaaacgcgg ttgccgcaat
    29641 tcctgttgct gttttcctga atgccggttt ttcctgtgcg ttggcgcaag aggctggttt
    29701 tgcctgcaaa agatcgcgta tcaccagcaa gacagcgagc acaatcaggg cacaggcata
    29761 gaaggtaggg aaggcacttg caccgggatc cgtttcaatg gcagttgctg gaaaatcagc
    29821 ggcattaatc atcaccgcgc cagcaaccac acaaagtaac agcgccacca ccatcgttga
    29881 tgaaaggcgc ttgcggtgcg gtacggcgag ttgagcaaca gactgttttg tgttcatcaa
    29941 ccgtacctca ggtttatttt ttcaggccca ggcgagtcag caactcgtca aagtactttt
    30001 cctgttcgct gatttgggtc tggaagctgg cagcgtcaag ccatgcatag ttgagattca
    30061 acttttgcag tgcatcctgg aatgcaggct cttcagccgt ctcttttgct gcctttgcca
    30121 gaacatccac cacatcctgc ggcgtttttt gcgacacaat caggccgcgc caggtgccga
    30181 tggagagatc aacgcctttc tcttttaacg tcgggacgtc aggcatggtt ttcattcgct
    30241 catccgccat cactaccagt gtcttcagct tgccgccatt cacatggttg ataacttctc
    30301 ctgggcttac ggaaaccgct tcaatgtgcc cacctaacag gcctgtaatg gcaggggctg
    30361 cgccgtcata cgggacatga gaaaactttg cgcccgtttt gtcttccagt gcagctgccg
    30421 ccagatgcca gatagctccg gtgcctgagt taccaatgcg tacttttccg ggattcgctt
    30481 tggcgtaagt cataaattct tcatagctat tccacggcgc atcggcacgg actgtgatag
    30541 cagccggatc cgcattcaga cgggcaatgg gtttaaagtc gctggtttta aaacgcacca
    30601 ttccgaggct gggaagggtg gttagttcaa ccgtgcctaa accaattttg taaccgttag
    30661 ggcgggcagc ggcgatttca ctcaggccga tagcaccgcc tccgccaggt ttattgataa
    30721 ccccgatgct gacgggtaaa tggtttttgg cggcatcagc aaaagctcgg gcaaccagat
    30781 ccgtaccgcc tccggcagcg tagggtacga ctaactcgat ctgtttggtt ggccaggaag
    30841 ccgcctgtac aggcgcaatg ctgagggcag cgcctgcaag aagtaccatt gcagccattt
    30901 ttttcggtaa ggtaagatgt tttatcattg cttttatatc cttatttatc agttagttgc
    30961 tatttaaatt gttccatgcg ttcataaaca gtactgcatt ttcatagact tgctctggcg
    31021 tcatgccggg gtgatatagc gcagaaccga ggccaaagcc gtttgcgccg tggctcagat
    31081 aacgcgccat ggtttccggt gtgataccgc caaccggtaa catcggaacg gattgtggaa
    31141 taaccgcacg ccaggctttg gtaacttctg gcgtaatttg ttcagccggg aacagcttga
    31201 tggcattagc accatgttca atggcagcaa aggcttcggt gggcgtggcg acgccaggcg
    31261 cacaatacat acccagcgca cgggcgcgac ggatcaccgc agtatcgcaa tgcggcatca
    31321 cgataatctg gcccccagcc tctttaaccg caaccacctg ctctaccgaa agcacagttc
    31381 cggctccgac caggcagtcc ttaggcaggg cgtcgcgcag taaactgata ctttgcaacg
    31441 cctccggcga gttcatagga atctcaatca ggcggaatcc ggcggcgtac agtttttcac
    31501 cgatggcgac aatctcatcc ggtttcacac cgcgtaaaat ggcgactaac ccggtttttt
    31561 gcaaaagcgt attaaatggc atgggtacat tccctggcgt tggtggacag taacccggca
    31621 gcgtgggcga ttcgccataa tccctgttca gtggcattgg gaatggcgtg tatctgtctg
    31681 tcgggaaaaa cgtggctgaa tgcttgtgaa tagcggctgt tcagatttgc gctaccgatt
    31741 aaggcgatcg gtgttgtggc gggtaaatca cgtagcaaac tggatgccag accacataat
    31801 tcatggccta tcagtaatcc ggaaaggtaa tccccctgcg aggacgacgt aagattactg
    31861 gtcagcaggc gcgaacgggt cgaaaacagc gtcgataata atccggtctg atgatgctct
    31921 tgtgctgctg tcagcccgtg ggtgaaggca tcccagtgcg gttcatctcc aggatgcatg
    31981 gtggcaccga gaatcgaatg gcgggataaa acatcaaata actcgcccgt cataaaggtt
    32041 cggaaatcgg taatggtgtt gttttcaact actgcccatt tcgcatgtgt gccgggtaag
    32101 cctatcagca ctggcatacc ctgatggatc gcgttttgca gggccggttc catcgagata
    32161 gcaccgaaga tttgtgtctc ttcgccacgc atcacttcgg gcatttcacc ttctttaatc
    32221 acgccaggaa tgatgtgcag cattgtctgc tgctggtgga taacaggcgt caactgcttc
    32281 gccagaccct cgagagtgac cgggcaaggt aaataagctg ctgattgcca cccttgcgca
    32341 ctgccgagca tgccgcaagc cagtaccggg catggggttt gtgccagcca gtcgccgcag
    32401 acacgtaaaa acgtatcgtg gaaagcgtcc tcacgtgttg ctgcctggga gggtaatttc
    32461 atgatccccc aggggaattc acgtttttct tgtggatttg gtgattcgcc aaaacgccat
    32521 gcccgaagcg atgacgttcc ccagtccagg gcaataaatg agggtgctcc cggcattagt
    32581 cgtttccttt aatcatggtt tatttatctt tgcggacgtg cttaccccag cccagttcag
    32641 cggatatctc ttctgcacag gatttgacgt aaggtgccag ttcttccaga cgcgccagcg
    32701 acatatacgt ggtggtactg gcgacagaaa tagccgccac aatttcatcc cgggcgttgt
    32761 aaaccggcgc tgcaacgcaa cggatagtcg gctcgttctc ttccagatca aaagcaaaac
    32821 cactggcggc ataacgacgc atgttctgaa taaatgcgcc cagttttgtt tcatcaccac
    32881 aggtcttaaa cagcgtgcgc cactcttctt ctgttcggtt gaggatcaat gccttgccga
    32941 tgccggtgat tgccagtggc atgcgatggc ctgggcgtga gcgcatttcc agcccgcgct
    33001 gactgttgat cttctccaga tacagcactt cgtcgccttc aacgatcccc agatgcacgg
    33061 tgtccagggt gtaacgggca aggcgttgca aaacgggctg ggcaatttcg tacagtgagg
    33121 tgctttccag tgctctggca ccgaattcga tcagtttggc acccagttgg tagccctgcg
    33181 ttgggacatg gcgcaggtag cgttgttcca ccagaacggt gactaaacga tgggttgtgc
    33241 tccgggtcgt gccggtgaac tcgccgatac tttttaggtc atggcagccg ttataaacag
    33301 cattaagaac cgccagtcca cgtaataacg tttgtgcgcc tgcggtggtt gaagaggttt
    33361 ctgccatacg tttctcctga tgatattgct gaagtgttcc acataatgac gtggggaaaa
    33421 ggtgatatat cccacaatat aaactccatc tcatattgtg ggataaaata atgaaaatta
    33481 acgatcgttt tgcgtagcgc ctcgcaggaa acttctttca aactggacgt ttgtacagca
    33541 caattctatt ttgtgcgggt aagttgttgg gtcaggaggc tttgtggatt tctcaatcat
    33601 tgtttatgcc attattgcgc tggttagtgt ggtcattggc tggctgtttg ccagttatca
    33661 acatgcgcag caaaaagccg agcaattagc tgaacgtgaa gagatggtcg cggagttaag
    33721 cgcggcaaaa caacaaatta cccaaagcga gcactggcgc gcagagtgtg aattgctcaa
    33781 taacgaagtg cgcagcctgc aaagtattaa cacctcgctg gaggccgatc tgcgtgaagt
    33841 gaccacgcgg atggaagccg cacagcaaca cgctgacgat aaaattcgcc agatgatcaa
    33901 tagcgaacag cgcctcagtg agcaatttga aaaccttgcc aaccgtattt ttgaacacag
    33961 taatcgtcgg gttgatgagc aaaatcgcca gagtctgaac agtcttttgt cgccgttgcg
    34021 tgaacaactg gatggtttcc gccgtcaggt ccaggacagc tttggtaaag aagcccaaga
    34081 acgtcatacc ctgactcacg aaatccgcaa cctccagcaa ctcaacgccc agatggccca
    34141 ggaagcgatc aacctgacgc gtgcgctgaa aggcgacaat aaaacccagg gcaactgggg
    34201 cgaggtagta ttgacgcggg tgctggaggc ttccggtctg cgtgaagggt atgaatatga
    34261 aacccaggtc agcatcgaaa atgacgcccg ctcgcggatg cagccggatg tcatcgtgcg
    34321 gttgccgcag ggaaaagatg tggtgatcga cgccaaaatg acgctggtcg cctatgaacg
    34381 ctatttcaac gccgaagatg actactcccg cgaaagcgcg ctacaggaac atattgcgtc
    34441 ggtgcgtaac catatccgtt tgctgggacg caaagattat caacagctgt cggggctgcg
    34501 aactctggat tacgtgctga tgtttattcc cgttgaaccc gcttttttac tggcgcttga
    34561 ccgtcagccg gagcttatca ccgaagcatt gaaaaacaat attatgctgg tgagccccac
    34621 cacgctgctg gtggcgttgc gtactatcgc caacctgtgg cgttatgagc atcaaagtcg
    34681 taacgcccag cacatcgccg atcgcgccag caagctgtac gacaaaatgc gcttgtttgt
    34741 cgatgacatg tccgcgatcg gtcaaagtct cgacaaagcg caggataact atcgccaggc
    34801 aatgaaaaaa ctctcttcag gtcgcggaaa tgtgctggcg caggcagaag cgtttcgcgg
    34861 tttaggggta gaaattaaac gcgagattaa tccggatttg gctgaacagg cggtgagcca
    34921 ggatgaagag tatcgacttc ggtcggttcc ggagcagccg aatgatgaag cttatcaacg
    34981 cgatgatgaa tataatcagc agtcgcgcta gcccattggg ggtagttaag ccgggtagaa
    35041 atctagggca tcgacgccca atctgttaca cttctggaac aattttttga tgagcaggca
    35101 ttgagatggt ggataagtca caagaaacga cgcactttgg ttttcagacc gtcgcgaagg
    35161 aacaaaaagc ggatatggtc gcccacgttt tccattccgt ggcatcaaaa tacgatgtca
    35221 tgaatgattt gatgtcattc ggtattcatc gtttgtggaa gcgattcacg attgattgca
    35281 gcggcgtacg ccgtgggcag accgtgctgg atctggctgg tggcaccggc gacctgacag
    35341 cgaaattctc tcgcctggtc ggagaaactg gcaaagtggt ccttgctgat atcaatgaat
    35401 ccatgctcaa aatgggccgt gagaagctgc gtaatatcgg tgtgattggc aacgttgagt
    35461 atgttcaggc gaacgctgag gcgctgccgt tcccggataa cacctttgat tgcatcacca
    35521 tttcgtttgg tctgcgtaac gtcaccgaca aagataaagc actgcgttca atgtatcgcg
    35581 tgctgaaacc cggcggccgc ctgctggtgc ttgagttctc gaagccaatt atcgagccgc
    35641 tgagcaaagc ctatgatgca tactccttcc atgtgctgcc gcgtattggc tcactggtcg
    35701 cgaacgacgc cgacagctac cgttatctgg cagaatccat ccgtatgcat cccgatcagg
    35761 ataccctgaa aaccatgatg caggatgccg gattcgaaag tgtcgactac tacaatctga
    35821 cggcaggggt tgtggcgctg catcgtggtt ataagttctg acaggagacc ggaaatgcct
    35881 tttaaacctt tagtgacggc aggaattgaa agtctgctca acaccttcct gtatcgctca
    35941 cccgcgctga aaacggcccg ctcgcgtctg ttgggcaaag tgctgcgcgt ggaggtaaaa
    36001 ggcttttcga cgtcattgat tctggtgttc agcgaacgcc aggtcgatgt actgggcgaa
    36061 tgggcaggcg atgctgactg caccgttatc gcttacgcca gtgtgttgcc gaaacttcgc
    36121 gatcgccagc agcttgccgc actgattcgc agtggtgagc tggaagtgca gggtgatatt
    36181 caggtggtgc aaaacttcgt tgcgctggca gatctggcag agttcgaccc tgcggaactg
    36241 ctggcccctt ataccggtga tatcgccgct gaaggtatca gcaaagccct gcgcggaggc
    36301 gcaaagttcc tgcatcacgg cattaagcgc cagcaacgtt atgtggcgga agccattact
    36361 gaagagtggc gtatggcacc cggtccgctt gaagtggcct ggtttgcgga agagactgct
    36421 gccgtcgagc gtgctgttga tgccctgacc aaacggctgg aaaaactgga ggctaaatga
    36481 cgccaggtga agtacggcgc ctatatttca tcattcgcac ttttttaagc tacggacttg
    36541 atgaactgat ccccaaaatg cgtatcaccc tgccgctacg gctatggcga tactcattat
    36601 tctggatgcc aaatcggcat aaagacaaac ctttaggtga gcgactacga ctggccctgc
    36661 aagaactggg accggtatgg atcaagttcg ggcagatgtt atcaacccgc cgcgatcttt
    36721 ttccgccgca tattgccgat cagctggcgt tattgcagga caaagtcgct ccgtttgatg
    36781 gcaagctggc gaagcagcag attgaagctg caatgggcgg cttgccggta gaagcgtggt
    36841 ttgacgattt tgaaatcaag ccgctggctt ctgcttctat cgcccaggtt cataccgcgc
    36901 gattgaaatc gaatggtaaa gaggtggtga ttaaagtcat ccgcccggat attttgccgg
    36961 ttattaaagc ggatctgaaa cttatctacc ggctggctcg ctgggtgccg cgtttgctgc
    37021 cggatggtcg ccgtctgcgc ccaaccgaag tggtgcgcga gtacgaaaag accttgattg
    37081 atgaactgaa tttgctgcgg gaatctgcca acgccattca gcttcgacgc aattttgaag
    37141 acagcccgat gctctacatc ccggaagttt accctgacta ttgtagtgaa gggatgatgg
    37201 tgatggagcg tatttacggc attccggtgt ctgatgttgc gacgctggag aaaaacggca
    37261 caaacatgaa attgctggcg gaacgcggcg tgcaggtgtt cttcactcag gtctttcgcg
    37321 acagcttttt ccatgctgat atgcacccag gcaacatctt cgtaagctat gaacacccgg
    37381 aaaacccgaa atatatcggc attgattgcg ggattgttgg ctcgctaaac aaagaagata
    37441 aacgctatct ggcagaaaac tttatcgcct tctttaatcg cgactatcgc aaagtggcag
    37501 agctacacgt cgattctggc tgggtgccac cagataccaa cgttgaagag ttcgaatttg
    37561 ccattcgtac ggtctgtgaa cctatctttg agaaaccgct ggccgaaatt tcgtttggac
    37621 atgtactgtt aaatctgttt aatacggcgc gtcgcttcaa tatggaagtg cagccgcaac
    37681 tggtgttact ccagaaaacc ctgctctacg tcgaaggggt aggacgccag ctttatccgc
    37741 aactcgattt atggaaaacg gcgaagcctt tcctggagtc gtggattaaa gatcaggtcg
    37801 gtattcctgc gctggtgaga gcatttaaag aaaaagcgcc gttctgggtc gaaaaaatgc
    37861 cagaactgcc tgaactggtt tacgacagtt tgcgccaggg caagtattta cagcatagtg
    37921 ttgataagat tgcccgcgag cttcagtcaa atcatgtacg tcagggacaa tcgcgttatt
    37981 ttctcggaat tggcgctacg ttagtattaa gttgcacatt cttgttggtc agccgacctg
    38041 aatgggggct gatgcccggc tggttaatgg caggtggtct gatcgcctgg tttgtcggtt
    38101 ggcgcaaaac acgctgattt tttcatcgct caaggcgggc cgtgtaacgt ataatgcggc
    38161 tttgtttaat catcatctac cacagaggaa catgtatggg tggtatcagt atttggcagt
    38221 tattgattat tgccgtcatc gttgtactgc tttttggcac caaaaagctc ggctccatcg
    38281 gttccgatct tggtgcgtcg atcaaaggct ttaaaaaagc aatgagcgat gatgaaccaa
    38341 agcaggataa aatcagccag gatgctgatt ttactgcgaa aactatcgcc gataagcagg
    38401 cggatacgaa tcaggaacag gctaaaacag aagacgcgaa gcgccacgat aaagagcagg
    38461 tgtaatctgt gtttgatatc ggttttagcg aactgctatt ggtgttcatc atcggcctcg
    38521 tcgttctggg gccgcaacga ctgcctgtgg cggtaaaaac ggtagcgggc tggattcgtg
    38581 cgttgcgttc actggcgaca acggtgcaga acgaactgac ccaggagtta aaactccagg
    38641 agtttcagga cagtctgaaa aaggttgaaa aggcgagcct cactaacctg acgcccgaac
    38701 tgaaagcgtc gatggatgaa ttacgccagg ctgcggagtc gatgaagcgt tcctacgttg
    38761 caaacgatcc tgaaaaggcg agcgatgaag cgcacaccat ccataacccg gtggtgaaag
    38821 acaatgaaac tgcgcatgaa ggcgtaacgc ctgctgctgc acaaacgcag gccagttcgc
    38881 cggaacagaa gccagaaacc acgccagagc cggtggtaaa acctgctgcg gacgctgaac
    38941 cgaaaaccgc tgcaccttcc ccttcgtcga gtgataaacc gtaaacatgt ctgtagaaga
    39001 tactcaaccg cttatcacgc atctgattga gctgcgtaag cgtctgctga actgcattat
    39061 ctcggtgatc gtgatattcc tgtgtctggt ctatttcgcc aatgacatct atcacctggt
    39121 atccgcgcca ttgatcaagc agttgccgca aggttcaacg atgatcgcca ccgacgtggc
    39181 ctcaccgttc tttacgccga tcaagctgac ctttatggtg tcgctgattc tgtcagcgcc
    39241 ggtgattctc tatcaggtgt gggcgtttat cgccccagcg ctgtataagc atgaacgtcg
    39301 cctggtggtg ccgctgctgg tttccagctc tctgctgttt tatatcggca tggcgttcgc
    39361 ctactttgtg gtctttccgc tggcatttgg cttccttgcc aataccgcgc cggaaggggt
    39421 acaggtatcc accgacatcg cgagctattt aagcttcgtt atggcgctgt ttatggcgtt
    39481 tggtgtctcc tttgaagtgc cggtggcaat tgtgctgctg tgctggatgg ggattacctc
    39541 gccagaagac ttacgcaaaa aacgcccgta tgtgctggtt ggtgcattcg ttgtcgggat
    39601 gttgctgacg ccgccggatg tcttctcgca aacgctgttg gcgatcccga tgtactgcct
    39661 gtttgaaatc ggtgtcttct tctcacgctt ttacgttggt aaagggcgaa accgggaaga
    39721 ggaaaacgac gctgaagcag aaagcgaaaa aactgaagaa taaattcaac cgcccgtcag
    39781 ggcggttgtc atatggagta caggatgttt gatatcggcg ttaatttgac cagctcgcaa
    39841 tttgcgaaag accgtgatga tgttgtagcg cgcgcttttg acgcgggagt taatgggcta
    39901 ctcatcaccg gtaccaatct gcgtgaaagc cagcaggcgc aaaagctggc gcgtcagtat
    39961 tcgtcctgtt ggtcaacggc gggcgtacat cctcacgaca gcagccagtg gcaagctgtg
    40021 actgaagaag cgattattga gctggccgcg cagccagaag tggtggcgat tggtgaatgt
    40081 ggtctcgact ttaaccgcaa cttttcgacg ccggaagagc aggaactcgc ttttgttgcc
    40141 cagctacgca ttgccgcaga attaaacatg ccggtattta tgcactgtcg cgatgcccac
    40201 gagcggttta tgacattgct ggagccgtgg ctggataaac tgcctggtgc ggttcttcat
    40261 tgctttaccg gcacacgcga agagatgcag gcgtgcgtgg cgcatggaat ttatatcggc
    40321 attaccggtt gggtttgcga tgaacgacgc gggctggagc tgcgggaatt gttgccgttg
    40381 attccggcgg agaaattgct gatcgaaact gatgcgccgt atctgctccc tcgcgatctc
    40441 acgccaaagc catcatcccg gcgcaacgag ccagcccatc tgccccatat tttgcaacgt
    40501 attgcgcact ggcgtggaga agatgccgca tggctggctg ccaccacgga tgccaatgtc
    40561 aaaacactgt ttgggattgc gttttagagt ttgcggaact cggtgttctt cacactgtgc
    40621 ttaatctctt tattaataag attaagcaat agcatggagc gagcctcacc atcgggttcg
    40681 gtgaaaatgg cctgaaaacc ttcgaacgcg ccttcagtaa taatcacttt atcaccagga
    40741 tacggcgtcg atggatcgac aatatctttc ggtttataca ctgataactg atgaatcacc
    40801 gccgatggga ctatcgctgg cgacgcgcca aagcgcacga agtggctgac accacgagtc
    40861 gcgttgatag tcgtggtatg aatcacttcc ggatcgaact ccacaaacaa gtagttgggg
    40921 aacaatggct cactgactgc agtacgtttg ccacgcacga ttttttccag ggtgatcatc
    40981 ggtgccaggc aattcacagc ctgtctttcg aggtgttcct gggcacgttg aagttgcccg
    41041 cgcttgcagt acagtaaata ccaggattgc ataatgactc ttatccgttt aatcggggcg
    41101 caaggatagc aaaagcttta cgctaagtta attatattcc ccggtttgcg ttataccgtc
    41161 agagttcacg ctaatttaac aaatttacag catcgcaaag atgaacgccg tataatgggc
    41221 gcagattaag aggctacaat ggacgccatg aaatataacg atttacgcga cttcttgacg
    41281 ctgcttgaac agcagggtga gctaaaacgt atcacgctcc cggtggatcc gcacctggaa
    41341 atcactgaaa ttgctgaccg cactttgcgt gccggtgggc ctgcgctgtt gttcgaaaac
    41401 cctaaaggct actcaatgcc ggtgctgtgc aacctgttcg gtacgccaaa gcgcgtggcg
    41461 atgggcatgg ggcaggagga tgtttcggcg ctgcgtgaag ttggtaaatt attggcgttt
    41521 ctgaaagagc cggagccgcc aaaaggtttc cgcgacctgt ttgataaact gccgcagttt
    41581 aagcaagtat tgaacatgcc gacaaagcga ctgcgtggtg caccctgcca acaaaaaatc
    41641 gtctctggcg atgacgtcga tctcaatcgc attcccatta tgacctgctg gccggaagat
    41701 gccgcgccgc tgattacctg ggggctcacc gtaacgcgcg gcccgcataa agagcggcag
    41761 aatctgggca tttatcgcca gcagctaatt ggtaaaaaca aactgattat gcgctggctg
    41821 tcgcatcgcg gcggcgcgtt ggattatcag gagtggtgtg cggcgcatcc gggcgaacgt
    41881 ttcccggttt ctgtggcgct gggtgccgat cctgccacga ttctcggtgc agtcaccccc
    41941 gttccggata cgctttcaga gtatgcgttt gccggattgc tgcgcggcac caagaccgaa
    42001 gtggtgaagt gtatctccaa tgaccttgaa gtgcccgcca gtgcggagat tgtgctggaa
    42061 gggtatatcg aacaagacga aactgcgccg gaagggccgt atggcgacca caccggttac
    42121 tataatgaag tcgatagttt cccggtattt accgtgacgc atattaccca gcgtgaagat
    42181 gcgatttatc attccaccta taccgggcgt ccgccagatg aacctgcggt actgggcgta
    42241 gcactgaacg aagtgttcgt gccgattctg caaaaacagt tcccggaaat tgtcgatttt
    42301 tacctgccgc cggaaggctg ttcttatcgt ctggcggtag tgacgatcaa aaaacagtac
    42361 gccggacacg cgaagcgcgt catgatgggc gtctggtcgt tcttacgcca gtttatgtac
    42421 actaaatttg tgatcgtttg cgatgatgac gtcaacgccc gcgactggaa cgatgtgatt
    42481 tgggcgatta ccacccgtat ggacccagcg cgggataccg ttctggtaga aaatacgcct
    42541 attgattatc tggattttgc ctcccctgtc tccgggctgg gttcaaaaat ggggctggat
    42601 gccacgaata aatggccggg ggaaacccag cgtgaatggg gacgtcccat caaaaaagat
    42661 ccagatgttg tcgcgcatat tgacgccatt tgggatgaac tggctatttt taacaacggt
    42721 aaaagcgcct gatgcgcgtt tgttttgccc tatttatcga tccgacagag aaagcgcatg
    42781 acaaccttaa gctgtaaagt gacctcggta gaagctatca cggataccgt atatcgtgtc
    42841 cgcatcgtgc cagacgcggc cttttctttt cgtgctggtc agtatttgat ggtagtgatg
    42901 gatgagcgcg acaaacgtcc gttctcaatg gcttcgacgc cagatgaaaa agggtttatc
    42961 gagctgcata ttggcgcttc tgaaatcaac ctttacgcga aagcagtcat ggaccgcatc
    43021 ctcaaagatc atcaaatcgt ggtcgatatt ccccacggag aagcgtggct gcgcgatgat
    43081 gaagagcgtc cgatgatttt gattgcgggc ggcaccgggt tctcttatgc ccgctcgatt
    43141 ttgctgacag cgttggcgcg taacccaaac cgtgatatca ccatttactg gggcgggcgt
    43201 gaagagcatc atctgtatga tctctgcgag cttgaggcgc tttcgttgaa gcatcctggt
    43261 ctacaagtgg tgccggtggt tgaacaaccg gaagcgggct ggcgtgggcg tactggcacc
    43321 gtgttaacgg cggtattgca ggatcatggt acgctggcag agcatgatat ctatattgcc
    43381 ggacgttttg agatggcgaa aattgcccgt gacctgtttt gcagcgagcg taatgcgcgg
    43441 gaagatcgcc tgtttggcga tgcgtttgca tttatctgag atataaaaaa cccgcccctg
    43501 acaggcggga agaacggcaa ctaaaacttt atacagtggc attactctac gttgcatccg
    43561 gcaaacagtg cccgatgcaa cgctgccgcg tcttatcggg cctacaaaag ttctgaaccg
    43621 tgggccggat aaggcgttca cgccgtatcc ggcatacagt gcccgatgcg acgctgccgc
    43681 gtcttatcgg gcctacaaaa gttctgaacc gtaggccgga taaggcgttc acgccgcatc
    43741 cggcaagtgg ttaaacccgc tcaaacaccg ttgcaatgcc ctgacccaga ccgatacaca
    43801 tcgtcgccag accaaactgg acgtctttgt gttccatcag attcagcagc gtggtgctga
    43861 tacgcgcacc ggaacaaccc agcggatgac ccagcgcgat cgcgccaccg ttgaggttga
    43921 tcttctcgtc aatctgctcc attagcccga gatctttaat acacggcagg atctgcgcgg
    43981 caaaggcttc gttcatctcg aatacgccga tatcgctggc agaaagcccc gcttttttca
    44041 gcgccagctt cgaggccgga accggaccat aacccataat cgatgggtca caacccacaa
    44101 ccgccatcga acgcatgcgg gcgcgcggct taagacctaa ttcacgggcg cggctttcac
    44161 tcatcaccag catggcagct gcgccatcgg aaagcgcaga agatgtaccc gccgttaccg
    44221 taccgctgac gggatcaaac gccggacgca gtgtggcgag ggcttccacg gtggtttccg
    44281 ggcgaatcac ttcgtcgtaa ttaaactgct tcaatacgcc gtcggcatcg tgaccaccgg
    44341 tcgggatgat ttcattttta aatgcgcctg actgcgtggc ggcccacgcg cgggcgtgtg
    44401 accgcgcggc aaaggcatcc tgcatttcac ggctgatacc gtgcatacgc gccagcattt
    44461 ctgccgttaa gcccatcatg cccgccgctt tggcgacatt acggctaagg ccggggtgaa
    44521 aatcgacgcc gtgactcatt ggcacatggc ccatatgctc cacgccgcca accagacatg
    44581 cctgcgcatc gccggtcatg atcattcgtg ctgcgtcatg cagtgcctgc atggatgaac
    44641 cacacaagcg attaacggta accgccggga cagagtgcgg cacttcagcc aacagtgccg
    44701 cgttacgggc gatgttaaaa ccctgctcca gtgtttgctg cacacaaccc cagtaaatat
    44761 cgtcgagggc cgctgcttcc agcgccgggt tacgcgccag caggctacgc attaaatgag
    44821 cggagagatc ttccgcacgc acgttacgaa aagcaccgcc cttcgaacgg cccatcgggg
    44881 tgcgaattgc atcgacaatg acaacctgtt ccattgtgac tccttaagcc gttttcaggt
    44941 cgccaaccgg acgggctggc tcaaccgggg gatagtacgg ttcgttatga cgtgctttat
    45001 tacgcatacc ttccggtact tcatacagcg ggccgaggtg ctgatattgc tgtgccatat
    45061 cgaggtattt tgcgctgccg agggtgtcca gccagcggaa cgcgccgccg tggaacggag
    45121 ggaagcccag gccgtagacc agcgccatat ccgcttctgc cggagtggcg ataatgccct
    45181 cttccagaca gcggaccact tcgttgacca tcgggatcat catgcgggcg ataatctctt
    45241 cctcgctgaa atcgcgcttc ggctggctca cttttgccag caggtcgtca acgacgacat
    45301 cttcttcttt ctccggctta cctttgctgt cttctttata acgccagaaa ccgaggccgt
    45361 tcttctgacc aaagcggttg gcattaaaca gcgcgtcgat ggcatcgcga taatctttct
    45421 gcatccgctg cgggaagcct gctgccatga ctgcctgggc atgatgtgcg gtgtcaatgc
    45481 ccacaacatc cagcagatat gccggaccca tcggccagcc aaactgtttt tccatcactt
    45541 tgtcgatctt gcggaaatcc gcgccgtcgc gcagcaactg gctgaaaccg gcgaaatagg
    45601 ggaacagtac acggttgacg aagaagccgg ggcagtcgtt aaccacaatc ggcgttttgc
    45661 ccattttgct cgcccaggcg acgactttcg cgatggtttc gtcggagctt ttctcgccgc
    45721 gaataatttc tactaacggc attcggtgga ccgggttaaa gaagtgcatc ccgcagaagt
    45781 tttccggacg ttccagcgcg ttggccagtt cgctgatagg aatggttgaa gtgttagacg
    45841 ccagtacggt atccgggcgt actttctgct cggtttctgc cagcacggct tttttcactt
    45901 tcgggttttc aacaaccgct tctaccacaa catccacgcg gtcaaacccg gcgtagtcga
    45961 gcgttgggtg aattgtggag atcacgccag ccagtttcag accatcgatc ttgccgcgct
    46021 caagctgctt gttcagcagt ttcgcggctt cggtcatgcc gagggttaac gacttgtcgt
    46081 tgatatcttt catgacaacc ggcacgcctt tccacgcaga ctggtaggcg atgccgccgc
    46141 ccataatgcc tgcgcccagc acagcggcct gtttcggggt ttcaacatct ttggtgagtt
    46201 tcttcgcttt accttttaca tattgatcgt taaggaaaat gccgaccagt gcgcgggctt
    46261 cgttggtatg cgccagcggg acaaaacttt tgttttccag gtttaaggct tcttcacgac
    46321 caaaacgggc cgccgcttca atggttttta ctgcggtgat gggggccgga taatgtttcc
    46381 ccgccgtttg cgcgaccatc cctttagcga tggtgaagct catggcggct tcaatcttgc
    46441 tcagttttag cggttccagc ttcggctgac gttttgcttt ccagtcgagg tcgccgttaa
    46501 tggcctggcg taaaatcgcc atcgcccctt caaccagttt ttctgctttg actacaccat
    46561 ccaccagacc gattttcagc gcctgatccg cgccgacatc tttaccggcg gcaatgattt
    46621 ccagcgcact gtcagcaccg agcatacgtg gcatacgtac agaaccgcca aagcccggca
    46681 tgatgcccag tttggtttcc ggcagaccga tgcgcagatc cggcgtcgcc agacgataat
    46741 cggtcgccag cacgcattcg cagccaccgc ccagcgcata gccgttgacg gcagcaatgg
    46801 tcggcaccgg gagatcttcc aggcgattaa acacgctatt ggcaaagtgc agccactgac
    46861 ttaactgttc ttcaggaacg aggaacaggg acaaaaattc ggtgatatca gcaccgacga
    46921 taaaggctgc tttgttcgaa cgcagcagca gcccttttag atctgattgc tgttccagca
    46981 cgccgatggc ctcgccgagg ctggcgacgg tggcagtgtc gagtttatta actgaacctg
    47041 gggcatcaaa taccagttcg gcaatgccat cttccagcca gtcaaggtac agggtgtcgc
    47101 ctttgtaaag catgtcagtc tcctgaatcc gcaaggtgat ctggtcgtac cagatgcatc
    47161 gaagtgtgta ttttgtgtta aaaatatgca aataaaatat taaggaaatg ccgatctgat
    47221 cacgctcggc agaaatcacg ctctggatga acgatgtgct aatatgcgga gacttaaggt
    47281 caaaaaaaca gaagggtaaa aaatggaatc actggcctcg ctctataaaa atcatatagc
    47341 taccttacag gaacggactc gcgatgcgct ggcgcgcttc aagctggatg cgttacttat
    47401 tcactccggc gaactgttca acgtttttct cgacgatcat ccctatccgt ttaaagtgaa
    47461 cccgcaattc aaagcgtggg tgccagtaac tcaggtgcca aactgctggc tgctggtgga
    47521 tggcgtgaat aagccgaaac tgtggttcta tctgccggtt gattactggc acaacgtcga
    47581 accgctgccg aactccttct ggactgaaga tgtagaagtg atcgcgctgc cgaaagccga
    47641 tggcattggt agtctgttgc ctgctgcgcg cggcaatatc ggttatatcg gtccggtgcc
    47701 agaacgtgcg ctgcaactgg gtattgaggc cagcaatatc aacccgaaag gggtgatcga
    47761 ctacctgcat tactatcgct ccttcaaaac cgagtacgaa ctggcctgta tgcgtgaagc
    47821 gcagaaaatg gcggtcaacg gtcatcgcgc ggcagaagaa gcgttccgtt ctggcatgag
    47881 cgagttcgat atcaacattg cctatctgac cgcgaccggt catcgtgata ccgacgtacc
    47941 ttatagcaac attgtggcgc ttaacgaaca cgctgcggtg ttgcattaca ccaaactgga
    48001 ccaccaggca ccggaagaga tgcgcagctt cctgctggat gccggggccg aatataacgg
    48061 ctatgcggct gatctaaccc gtacctggtc ggcaaaaagc gacaacgact acgcacagct
    48121 ggtgaaagac gtaaatgatg agcaactggc gctgatcgcc accatgaaag caggcgtcag
    48181 ctatgtggat taccatatcc agttccatca gcgcatcgcc aaactgctgc gtaaacatca
    48241 aatcatcacc gatatgagtg aagaagcgat ggtcgaaaac gatctcaccg gaccgtttat
    48301 gccgcacggt atcggccatc cgctgggcct gcaggtgcat gacgtagccg gttttatgca
    48361 ggatgatagc ggtacgcacc tcgcggcacc ggcaaaatat ccgtacctgc gctgcacccg
    48421 tattctccag ccgggcatgg tgttaaccat cgaaccgggt atctacttca tcgaatcgct
    48481 tctggcaccg tggcgtgaag ggcagttcag caaacacttc aactggcaga aaattgaagc
    48541 actgaaaccg ttcggcggca ttcgtatcga agacaacgtg gtgattcacg aaaacaacgt
    48601 ggaaaacatg acccgggatc tgaaactggc gtgatggaaa gctggttaat tcctgcggca
    48661 ccggtcatgg tcgttgaaga gatcaaaaag agccgcttca ttacgctgtt ggcgcatacc
    48721 gatggcgttg aggcggcaaa agcgtttgtt gaatcggtgc gggcagaaca ccccgatgcc
    48781 cgccaccatt gcgtggcgtg ggtggcgggt gcgccggatg attctcaaca gctgggtttc
    48841 tctgacgacg gggagccggc gggaacggca ggtaaaccga tgctcgccca actaatgggc
    48901 agcggcgtcg gggaaattac cgctgtggtg gtgcgctact acggcggcat attgcttggc
    48961 accggtgggt tagtgaaagc gtatggcggc ggcgtgaatc aggcgctgcg ccagctaacg
    49021 acccaacgca agacaccatt aaccgaatat actttgcaat gtgaatatag tcagttaacc
    49081 ggcattgaag cgttgctggg gcagtgtgac ggcaaaatta tcaacagtga ttatcaggca
    49141 ttcgttctgc tgcgggtggc gcttccggcg gcgaaagtgg ctgaattttc cgcaaagctg
    49201 gcggatttta gccgtggttc attgcaattg ttagcgattg aagaataatc cccacttcgt
    49261 tttgcagaac taaggaagcg gcagagatgc attttcgcgc cattacccga atcgttggac
    49321 tactggtcat cttattttca gggaccatga ttatccctgg gctggtagca ctcatctacc
    49381 gggatggagc gggccgcgct tttacccaga ccttttttgt cgccctcgcc attggctcta
    49441 tgctttggtg gccgaaccgc aaagagaagg gcgaactgaa atcccgtgag gggtttctga
    49501 tagtggtgct gttctggacc gtgctgggta gcgtcggtgc gctccctttt atcttctcgg
    49561 aaagcccgaa cctcacgatt accgatgcgt tttttgaatc tttctctggc ctgaccacca
    49621 cgggggccac tacgctggtg gggctggatt cgctccctca tgccatcctc ttttatcgcc
    49681 agatgctgca atggtttggc ggtatgggga tcatcgtgct ggcggttgcg atactgccta
    49741 tcctcggcgt gggtgggatg cagctctatc gcgcagaaat gcccggcccg ctgaaagata
    49801 acaaaatgcg cccgcgaatt gcggaaacgg cgaaaaccct gtggttgatt tatgtcttgc
    49861 tgaccgtcgc ctgtgcgctg gcgttgtggt ttgctggaat ggatgccttt gatgccatcg
    49921 gccatagctt tgcgaccatc gctattggcg gcttctcgac acatgatgcc agtatcggtt
    49981 atttcgacag cccgactatt aacactatca ttgctatctt cctgctgatc tctggctgta
    50041 actacggtct gcacttttca ctgttaagtg ggcgtagtct gaaggtttat tggcgcgatc
    50101 cggaatttcg catgtttatc ggcgtacagt tttcactggt ggttatttgt acactcgtac
    50161 tgtggtttca taatgtctac agttcggcgc tgatgacaat taaccaggcg tttttccagg
    50221 tggtgtcgat ggcgacaacc gccgggttta cgactgacag cattgcccgc tggccgctct
    50281 ttttgccggt actgctctta tgttcagctt ttatcggcgg ttgtgccggg tcaacgggcg
    50341 gtggcctgaa agtgatccgc atcctgctgc tgtttaagca ggggaaccgt gagctgaaac
    50401 gactggtgca tccgaacgcc gtctacagca ttaagctggg gaatcgcgca ctgccggaac
    50461 gtatcctcga agccgtttgg ggatttttct ccgcctatgc attggtgttt attgtcagta
    50521 tgctggcgat tatcgccacg ggcgtggatg acttttctgc ctttgcctcg gttgttgcaa
    50581 cattgaataa cctggggcca gggcttggcg tggttgctga taactttacc agtatgaacc
    50641 cggtggctaa atggatcctg attgccaaca tgctgtttgg tcgtctcgag gtctttacat
    50701 tgctggtgct ctttaccccg actttctggc gtgaatgatg gagtaatacg tgaaaacatt
    50761 aattcttttc tcaacaaggg acggacaaac gcgcgagatt gcctcctacc tggcttcgga
    50821 actgaaagaa ctggggatcc aggcggatgt cgccaatgtg caccgcattg aagaaccaca
    50881 gtgggaaaac tacgaccgtg tggtcattgg tgcttctatt cgctatggtc actaccactc
    50941 tgcgttccag gaatttgtca aaaagcatgc gacgcggctg aattcgatgc cgagcgcctt
    51001 ttactccgta aacctggtgg cgcgtaaacc ggagaagcgt actccacaga ccaacagcta
    51061 cgcgcggaag tttctgatga gttcacaatg gcgtcccgat cgctgcgcgg tcattgccgg
    51121 ggcgctgcgt tacccacgtt atcgctggta cgaccgcttt atgatcaaac tgattatgaa
    51181 gatgtcaggc ggtgaaacgg atacgcgcaa agaagttgtc tataccgatt gggagcaggt
    51241 ggcgaatttc gcccgagaaa tcgcccattt aaccgacaaa ccgacgctga aataagcata
    51301 aagaataaaa agtgcgcggt c
//
LOCUS       DADSYM010000009        51306 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2415
            SAMN08039579-rid11723173.denovo.009, whole genome shotgun sequence.
ACCESSION   DADSYM010000009 DADSYM010000000
VERSION     DADSYM010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039579
            Sequence Read Archive: SRR6362533
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 51306)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 51306)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980162.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 03:39:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,484
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 4,347
            CDSs (with protein)               :: 4,347
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 43 of 85
            Pseudo Genes (incomplete)         :: 37 of 85
            Pseudo Genes (internal stop)      :: 19 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..51306
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039579-rid11723173.denovo.009"
                     /strain="LGE2415"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8302 N 95.9628 W"
                     /collection_date="2015-08-24"
     gene            complement(26..2098)
                     /locus_tag="J1Y83_000445"
     CDS             complement(26..2098)
                     /locus_tag="J1Y83_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001591423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HBA7628631.1"
                     /translation="MSQLDEAILDALTHVTFPKGFAQAEPAWVVTVDGVDYPLWKTDA
                     LVVGSGAAGLRAAVELKRRQQNVLIATAGLYMGTSACSGSDKQTLFTAATAGNGDNFA
                     KLAEALASGGAMDHDTAYVEAVGSLHTLGGLQYLGLELPEDRYGAILRYQTDHDEAGR
                     ATSCGPRTSRLMVKVLLEEVQRLAIPMLTSATVIKLLHQRDENGEDRVAGAILATGHR
                     AHNPWGLAIVTAPNVVLATGGPGELYRDSVYPHKCFGSLGLALEEGLTLTNLTESQFG
                     IGTPRSTFPWNLSGTYVQVIPYIYSVDAEGNEYNFLANYYRTTQELASNIFRKGYQWP
                     FHATRVMDFGSSLLDMAVAQEQQSGRLVFMDFNRNPEVVPGDLPFSLDRLDDDVRAYL
                     ENNDALAPSPIERLQRMNPLSISLYKMHGYDLTTQPLQFAMNNQHMNGGIEVDIWGQT
                     SLPGCFAVGEVAGTHGVTRPGGAALNAGQVFAVRLARFIGCTQKRNIDGDIAQLAAPA
                     LASIREIITQAPDNGTGMPLSVVREKIQARMSDHAGFICHADKVRRATRDALLLNEFV
                     QRHGLAIKHVGEVAELFMWRHMALTSAAVLTQLTHYIDAGGGSRGARMVIDPQGKCLP
                     QTRRGVKEEWRFRSELAEDKNHKLTIQYSQGSFITEVKSLRMQPCINGIYFEKNWPDF
                     LKGDIYTQ"
     gene            complement(2095..3636)
                     /locus_tag="J1Y83_000446"
     CDS             complement(2095..3636)
                     /locus_tag="J1Y83_000446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001226973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl CoA:acetate/3-ketoacid CoA transferase"
                     /protein_id="HBA7628632.1"
                     /translation="MRKLSTAETLATQIQDGATIAISGNGGGMVEADHIMAAIEERFL
                     QTGHPRDLTLIHSLGIGDRDSKGTNRFAHAEMLKRIIAGHFTWSPKMQELVKSNAIEA
                     YCFPGGVIQALLREIGAGRPGLFTHVGLGSFVDPRNGGGKSNECTTDDLVELIEIDGE
                     TKLRYRPFKVDYAILRGTYADPRGNVSLEEEAIDMDSYSMALAAHNSGGKVFVQVRDV
                     LEAGTIEPRRVKLPGILVDGIVEHREQPQTYLGGYDLTISGQHRRLSSNDAIELVSHP
                     VRRLIARRAARELVAGASTNFGFGIPGGIPGVALREGVPYQSLWLSVEQGVHNGMMLD
                     DALFGCARNADAIIPSLDQFEFYSGGGIDITFLGMGEMDRYGNVNVSHLNGNLIGPGG
                     FLEIAQNARKVVFCGTFDAKGSKIDVTPDGLHIAQSGQIPKLVTLVEKITFSASYAQQ
                     SGQEVLYITERAVFQLTAEGVELIEIAPGVEIERDILPFMAFRPIIKHPRLMESSLFT
                     PMEDA"
     gene            complement(3646..4422)
                     /locus_tag="J1Y83_000447"
     CDS             complement(3646..4422)
                     /locus_tag="J1Y83_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001283531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HBA7628633.1"
                     /translation="MSDQPVLFSRAAASCRLTLNREDKCHAINEEMIESLDHYLNEIE
                     NDTALRLVELTATGDKFFCAGGDIKSWSAYSPLDMGRKWIKRGNDVFNRLRNLPQLTV
                     ANLNGHTIGGGIELALCCDIRIARPGAKFSNPEVMLGMVPGWMGIERVLNQVGPVVGR
                     QMLMLGKRLTAQEAQVANLIDEVVEKEQVESWMANQLAQLEKCGPVALAHIKQLILAL
                     ENKHADYSHQLLAGLMSATQDCQQATHAFAEKAGVSFHNQ"
     gene            complement(4432..5274)
                     /locus_tag="J1Y83_000448"
     CDS             complement(4432..5274)
                     /locus_tag="J1Y83_000448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001205218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HBA7628634.1"
                     /translation="MRDLLQRPDLFSINTATLGYKTPLPAIIDACAARGIGAIAPWRR
                     ELQGEDLQQIARQLAASNINVSGLCRSTYYTAPTLAERKLAIDDNRRALDDAAVLNAA
                     CYMQVVGGLPMGTKDLYEAREQVKQGIRQLLPHSKEVGVPIALEPLHPMTAADRSCLC
                     TLRQALDWCDELDPDGEFGLGVAVDVYHVWWDPDLASQILRAGKRILAFHVSDWLVPT
                     TDLVNDRGMPGDGVINIPSIRRLVENAGFNGAIELEIFSPYWWQKDINSTLDISVDRI
                     AHYC"
     gene            complement(5284..6075)
                     /locus_tag="J1Y83_000449"
     CDS             complement(5284..6075)
                     /locus_tag="J1Y83_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709993.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-ketoacyl-ACP reductase"
                     /protein_id="HBA7628635.1"
                     /translation="MKSTRPVAVITGAARGIGKGCALELARGGFNLLINDRPDADSVE
                     KLHATQQECLAEGVEVICFPADVGDLSLHEEMLDAAQNQWGRLDCLLNNAGISVKKRG
                     DLLDLEPDSFDQNIAINTRAPFFLAQAFSKRLLAQPKPEAELPHRSIIFVSSINAIML
                     AMNRGEYTIAKTAVSAAARLFAARLCNEQIGVYEVRPGLIKTDMTIPATAYYDELIAK
                     GLVPWGRWGYPADIASTVRAMAEGKLIYTCGQAVAIDGGLSMPRF"
     gene            6288..6935
                     /locus_tag="J1Y83_000450"
     CDS             6288..6935
                     /locus_tag="J1Y83_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709992.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBA7628636.1"
                     /translation="MYLDTSNVQSLKEKLLLCAVNEFAEYGYEGARVDNIVKAAGCSK
                     QTVYHHFGNKENLFIEVLEYTWNDIRQKEKALDFSDLPPQKAIEKIIDFTWGYYISNP
                     WFLKIVHSENQSKGVHYAKSQRLLEINHAHLQLMESLLDEGKKQNIFKPDIDPLQVNI
                     NIAALGGYYLINQHTLGLVYHISMVSPQALEARRKVIKETILSWLLVDPSSTARE"
     gene            complement(6919..7557)
                     /gene="ytfB"
                     /locus_tag="J1Y83_000451"
     CDS             complement(6919..7557)
                     /gene="ytfB"
                     /locus_tag="J1Y83_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418627.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein YtfB"
                     /protein_id="HBA7628637.1"
                     /translation="MPGRFELKPTLEKVWHAPDNFRFMDPLPPMHRRGIIIAAIVLVV
                     GFLLPSDDTPNAPVVTREAQLDIQSQSQPPTEEQLRAQLVTPQNDPDQVAPVAPEPIQ
                     EGQPEEQPQTTHTQPFQPDSGIDNQWRSYRVEPGKTMAQLFRDHGLPATDVYAMAQVE
                     GADKPLSNLQNGQMVKIRQNASGVVTGLTIDTGNNQQVLFTRQPDGSFIRAR"
     gene            7775..8395
                     /locus_tag="J1Y83_000452"
     CDS             7775..8395
                     /locus_tag="J1Y83_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313212.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HBA7628638.1"
                     /translation="MTTPTFDTIEAQASYGIGLQVGQQLSESGLEGLLPEALVAGIAD
                     ALEGKHPAVPVDVVHRALREIHERADAVRRQRFQAMAAEGVKYLEENAKKEGVNSTES
                     GLQFRVINQGEGAIPARTDRVRVHYTGKLIDGTVFDSSVARGEPAEFPVNGVIPGWIE
                     ALTLMPVGSKWELTIPQELAYGERGAGASIPPFSTLVFEVELLEIL"
     gene            8704..10116
                     /gene="cycA"
                     /locus_tag="J1Y83_000453"
                     /gene_synonym="dagA"
     CDS             8704..10116
                     /gene="cycA"
                     /locus_tag="J1Y83_000453"
                     /gene_synonym="dagA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine/D-alanine/glycine transporter"
                     /protein_id="HBA7628639.1"
                     /translation="MVDQVKVVADDQAPAEQSLRRNLTNRHIQLIAIGGAIGTGLFMG
                     SGKTISLAGPSIIFVYMIIGFMLFFVMRAMGELLLSNLEYKSFSDFASDLLGPWAGYF
                     TGWTYWFCWVVTGMADVVAITAYAQFWFPGLSDWVASLSVIILLLVLNLATVKMFGEM
                     EFWFAMIKIVAIVSLIVVGLVMVAMHFQSPTGVEASFAHLWNDGGWFPKGLSGFFAGF
                     QIAVFAFVGIELVGTTAAETKDPEKSLPRAINSIPIRIIMFYVFSLIVIMSVTPWSSV
                     VPEKSPFVELFVLVGLPAAASVINFVVLTSAASSANSGVFSTSRMLFGLAQEGVAPKA
                     FAKLSKRAVPAKGLTFSCICLLGGVVMLYVNPSVIGAFTMITTVSAILFMFVWTIILC
                     SYLVYRKQRPHLHEKSIYKMPLGKLMCWVCMAFFVFVLVLLTLEDDTRQALLVTPLWF
                     IALGLGWLFIGKKRAAELRK"
     gene            complement(10161..10823)
                     /gene="ytfE"
                     /locus_tag="J1Y83_000454"
     CDS             complement(10161..10823)
                     /gene="ytfE"
                     /locus_tag="J1Y83_000454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster repair protein YtfE"
                     /protein_id="HBA7628640.1"
                     /translation="MAYRDQPLGELALSIPRASALFRKYDMDYCCGGKQTLARAAARK
                     ELDVDVIEAELAKLAEQPIEKDWRSAPLAEIIDHIIVRYHDRHREQLPELILQATKVE
                     RVHADKPSVPKGLTKYLTMLHEELSSHMMKEEQILFPMIKQGMGSQAMGPISVMESEH
                     DEAGELLEVIKHTTNNVTPPPEACTTWKAMYNGINELIDDLMEHISLENNVLFPRALA
                     GE"
     gene            complement(10931..11896)
                     /locus_tag="J1Y83_000455"
     CDS             complement(10931..11896)
                     /locus_tag="J1Y83_000455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418631.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HBA7628641.1"
                     /translation="MISGVLYALLAGLMWGLIFVGPLIVPEYPAMLQSMGRYLALGLI
                     ALPIAWLGRVRLRQLARRDWLTALMLTMMGNLIYYFCLASAIQRTGAPVSTMIIGTLP
                     VVIPVFANLLYSQRDGKLAWGKLAPALVCIGIGLACVNIAELNHGLPDFDWARYTSGI
                     VLALVSVVCWAWYALRNARWLRENPDKHPMMWATAQALVTLPVSLIGYLVACYWLNMQ
                     TPDFSLPFGPRPLVFISLMVAIAVLCSWVGALCWNVASQRLPTVILGPLIVFETLAGL
                     LYTFLLRQQMPPLMTLSGIALLVIGVVIAVRAKPEKPLTESVSES"
     gene            complement(12005..12865)
                     /gene="qorB"
                     /locus_tag="J1Y83_000456"
     CDS             complement(12005..12865)
                     /gene="qorB"
                     /locus_tag="J1Y83_000456"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HBA7628642.1"
                     /translation="MIAITGATGQLGHYVIESLMKTVPASQIVAIVRNPAKAQALTAQ
                     GITVRQADYGDEAALTSALQGVEKLLLISSSEVGQRAPQHRNVINAAKAADVKFIAYT
                     SLLHADSSPLGLADEHIETEKMLADSGIVYTLLRNGWYTENYLASAPAALEHGVFIGA
                     AGDGKIASATRADYAVAAARVISEAGHEGKVYELAGDSAWTLTQLAAELTKQSGKSVT
                     YQNLSEADFAAALKSVGLPDGLADMLADSDVGASKGGLFDDSKTLSKLIGRPTTTLAE
                     SVSHLFNVNN"
     gene            12954..13334
                     /locus_tag="J1Y83_000457"
     CDS             12954..13334
                     /locus_tag="J1Y83_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418633.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HBA7628643.1"
                     /translation="MSQVSLSQQLKEGNLFAEQCPSREVLKHVTSRWGVLILVALREG
                     THRFSDLRRKMGGVSEKMLAQSLQALEQDGFLNRIAYPVVPPHVEYSLTPLGEQVSEK
                     VAALADWIELNLPEVLAVRDERAA"
     gene            complement(13452..15395)
                     /gene="cpdB"
                     /locus_tag="J1Y83_000458"
     CDS             complement(13452..15395)
                     /gene="cpdB"
                     /locus_tag="J1Y83_000458"
                     /EC_number="3.1.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2',3'-cyclic-nucleotide 2'-phosphodiesterase"
                     /protein_id="HBA7628644.1"
                     /translation="MIKFSATLLATLIAASVNAATVDLRIMETTDLHSNMMDFDYYKD
                     TATEKFGLVRTASLINDARNEVKNSVLVDNGDLIQGSPLADYMSAKGLKAGDIHPVYK
                     ALNTLDYTVGTLGNHEFNYGLDYLKNALAGAKFPYVNANVIDARTKQPMFTPYLIKDT
                     EVVDKDGKKQTLKIGYIGVVPPQIMGWDKANLFGKVTVNDITETVRKYVPEMREKGAD
                     VVVVLAHSGLSADPYKVMAENSVYYLSEIPGVNAIMFGHAHAVFPGKDFADIEGADIA
                     KGTLNGVPAVMPGMWGDHLGVVDLQLSNDSGKWQVTQAKAEARPIYDIANKKSLAAED
                     SKLVETLKADHDATRQFVSKPIGKSADNMYSYLALVQDDPTVQVVNNAQKAYVEHYIQ
                     GDPDLAKLPVLSAAAPFKVGGRKNDPASYVEVEKGQLTFRNAADLYLYPNTLIVVKAS
                     GKEVKEWLECSAGQFNQIDPNSTKPQSLINWDGFRTYNFDVIDGVNYQIDVTQPARYD
                     GECQMINANAERIKNLTFNGKPIDPNAMFLVATNNYRAYGGKFAGTGDSHIAFASPDE
                     NRSVLAAWIADESKRAGEIHPAADNNWRLAPIAGDKKLDIRFETSPSDKAAAFIKEKG
                     QYPMNKVATDDIGFAIYQVDLSK"
     gene            15585..16325
                     /gene="cysQ"
                     /locus_tag="J1Y83_000459"
     CDS             15585..16325
                     /gene="cysQ"
                     /locus_tag="J1Y83_000459"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'(2'),5'-bisphosphate nucleotidase CysQ"
                     /protein_id="HBA7628645.1"
                     /translation="MLDQVCQLARNAGDAIMQVYDGTKPMDVVSKADNSPVTAADIAA
                     HTVIMDGLRTLTPDIPVLSEEDPPGWEVRQHWQRYWLVDPLDGTKEFIKRNGEFTVNI
                     ALIDHGKPILGVVYAPVMNVMYSAAEGKAWKEECGVRKQIQVRDARPPLVVISRSHAD
                     AELKEYLQQLGEHQTTSIGSSLKFCLVAEGQAQLYPRFGPTNIWDTAAGHAVAAAAGA
                     HVHDWQGKPLDYTPRESFLNPGFRVSIY"
     gene            complement(16315..16872)
                     /locus_tag="J1Y83_000460"
     CDS             complement(16315..16872)
                     /locus_tag="J1Y83_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003830421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtfJ family protein"
                     /protein_id="HBA7628646.1"
                     /translation="MTLRKILALTCLLLPMMASAHQFETGQRVPPIGITDRGELVLDK
                     DQFSYKTWNSAQLVGKVRVLQHIAGRTSAKEKNATLIEAIKSAKLPHDRYQTTTIVNT
                     DDAIPGSGMFVRSSLESNKKLYPWSQFIVDSNGVARGAWQLDEESSAVVVLDKDGRVQ
                     WAKDGALTQEEVQQVMDLLHKLINK"
     gene            17197..17403
                     /locus_tag="J1Y83_000461"
     CDS             17197..17403
                     /locus_tag="J1Y83_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1107 domain-containing protein"
                     /protein_id="HBA7628647.1"
                     /translation="MKIFQRYNPLQVAKYVKILFRGRLYIKDVGAFEFDKGKILIPKV
                     KDKLHLSVMSEVNRQVMRLQTEMA"
     gene            complement(17481..18824)
                     /locus_tag="J1Y83_000462"
     CDS             complement(17481..18824)
                     /locus_tag="J1Y83_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HBA7628648.1"
                     /translation="MLNSILVILCLIAVSAFFSMSEISLAASRKIKLKLLADEGNINA
                     QRVLNMQENPGMFFTVVQIGLNAVAILGGIVGDAAFSPAFHSLFSRYMSAELSEQLSF
                     ILSFSLVTGMFILFADLTPKRIGMIAPEAVALRIINPMRFCLYVCTPLVWFFNGLANI
                     IFRIFKLPMVRKDDITSDDIYAVVEAGALAGVLRKQEHELIENVFELESRTVPSSMTP
                     RENVIWFDLHEDEQSLKNKVAEHPHSKFLVCNEDIDHIIGYVDSKDLLNRVLANQSLA
                     LNSGVQIRNTLIVPDTLTLSEALESFKTAGEDFAVIMNEYALVVGIITLNDVMTTLMG
                     DLVGQGLEEQIVARDENSWLIDGGTPIDDVMRVLDIDEFPQSGNYETIGGFMMFMLRK
                     IPKRTDSVKFAGYKFEVVDIDNYRIDQLLVTRIDSKATALSPKLPDAKDKEESVA"
     gene            complement(19147..19785)
                     /gene="msrA"
                     /locus_tag="J1Y83_000463"
     CDS             complement(19147..19785)
                     /gene="msrA"
                     /locus_tag="J1Y83_000463"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HBA7628649.1"
                     /translation="MSLFDKKHLVSPADALPGRNTPMPVATLHAVNGHSMTNVPDGME
                     IAIFAMGCFWGVERLFWQLPGVYSTAAGYTGGYTPNPTYREVCSGDTGHAEAVRIVYD
                     PSVISYEQLLQVFWENHDPAQGMRQGNDHGTQYRSAIYPLTPEQDAAARASLERFQAA
                     MLAADDDRRITTEIANATPFYYAEDDHQQYLHKNPYGYCGIGGIGVCLPPEA"
     gene            19991..21724
                     /gene="tamA"
                     /locus_tag="J1Y83_000464"
     CDS             19991..21724
                     /gene="tamA"
                     /locus_tag="J1Y83_000464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter assembly complex protein TamA"
                     /protein_id="HBA7628650.1"
                     /translation="MRYIRQLCCVSLLCLSGSAVAANVRLQVEGLSGELEKNVRAQLS
                     TIESDEVTPDRRFRARVDDAIREGLKALGYYQPTIEFDLRPPPKKGRQVLIAKVTPGV
                     PVLIGGTDVVLRGGARTDKDYLKLLDTRPAIGTVLNQGDYENFKKSLTSIALRKGYFD
                     SEFTKAQLGIALGLHKAFWDIDYNSGERYRFGHVTFEGSQIRDEYLQNLVPFKEGDEY
                     ESKDLAELNRRLSATGWFNSVVVAPQFDKARETKVLPLTGVVSPRTENTIETGVGYST
                     DVGPRVKATWKKPWMNSYGHSLTTSTSISAPEQILDFSYKMPLLKNPLEQYYLVQGGF
                     KRTDLNDTESDSTTLVASRYWDLSSGWQRAINLRWSFDHFTQGEITNTTMLFYPGVMI
                     SRTRSRGGLMPTWGDSQRYSIDYSNTAWGSDVDFSVFQAQNVWIRTLYDRHRFVTRGT
                     LGWIETGDFDKVPPDLRFFAGGDRSIRGYKYKSIAPKYANGDLKGASKLITGSLEYQY
                     NVTGKWWGAVFVDSGEAVSDIRRSDFKTGTGVGVRWESPVGPIKLDFAVPVADKDEHG
                     LQFYIGLGPEL"
     gene            21721..25500
                     /gene="tamB"
                     /locus_tag="J1Y83_000465"
     CDS             21721..25500
                     /gene="tamB"
                     /locus_tag="J1Y83_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter assembly complex protein TamB"
                     /protein_id="HBA7628651.1"
                     /translation="MSLWKKISLGVVIVILLLLGSVAFLVGTTSGLHLVFKAADRWVP
                     GLDIGKVTGGWRDLTLSDVRYEQPGVAVKAGNLHLAVGLESLWNSSVCINDLALKDIQ
                     VNIDSKKMPPSEQVEEEEDSGPLDLSTPYPITLTRVALDNVNVKIDDTTVSVMDFTSG
                     LNWQEKTLTLKPTSLKGLLIALPKVAEAAQEEVVEPKIENPQPEEKPLGETLKDLFSR
                     PVLPEMTDVHLPLNLNIEEFKGEQLRVTGDTDITVSTMLLKVSSIDGNTKLDALDIDS
                     SQGIVNASGTAQLSDNWPVDITLNSTLNVEPLKGEKVKLKVGGALREQLEIGVNLSGP
                     VDMDLRAQTRLAEAGLPLNVEVNSKQLYWPFTGEKQYQADDLKLKLTGKMTDYAISMR
                     TAVKGQEIPPATITLDAKGNEQQVNLDKLTVAALEGKTELKALLDWQQAISWRGELTL
                     NGINTAKEIPEWPSKLNGLIKTRGSLYGGTWQMEVPELKLTGNVKQNKVNVDGTLKGN
                     SYMQWMIPGLHLELGPNSAEVKGELGVKDLNLDATINAPGLDNALPGLGGTAKGLVKV
                     RGTVEAPQLLADITARGLRWQELSVAQVRVEGDIKSTDQIAGKLDVRVEQISQPDVNI
                     NLVTLNAKGSEKQHELQLRIQGEPVSGQLNLAGSFDRKEERWKGTLSNTRFQTPVGPW
                     SLTRDIALDYRNKEQKISIGPHCWLNPNAELCVPQTIDAGAEGRAVVNLNRFDLAMLK
                     PFMPETTQASGIFTGKADVAWDTTKEGLPQGSITLSGRNVQVTQTVNDAALPVAFQTL
                     NLTAELRNNRAELGWTIRLTNNGQFDGQVQVTDPQGRRNLGGNVNIRNFNLAMINPIF
                     TRGEKAAGMVSANLRLGGDVQSPQLFGQLQVTGVDIDGNFMPFDMQPSQLAVNFNGMR
                     STLAGTVRTQQGEIYLNGDADWSQIENWRARVTAKGSKVRITVPPMVRMDVSPDVVFE
                     ATPNLFTLDGRVDVPWARIVVHDLPESAVGVSSDVVMLNDNLQPEEPKTASIPINSNL
                     IVHVGNNVRIDAFGLKARLTGDLNVVQDKQGLGLNGQINIPEGRFHAYGQDLIVRKGE
                     LLFSGPPDQPYLNIEAIRNPDATEDDVIAGVRVTGLADEPKAEIFSDPAMSQQAALSY
                     LLRGQGLESDQSDSAAMTSMLIGLGVAQSGQIVGKIGETFGVSNLALDTEGVGDSSQV
                     VVSGYVLPGLQVKYGVGIFDSIATLTLRYRLMPKLYLEAVSGVDQALDLLYQFEF"
     gene            25503..25844
                     /locus_tag="J1Y83_000466"
     CDS             25503..25844
                     /locus_tag="J1Y83_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcyclotransferase"
                     /protein_id="HBA7628652.1"
                     /translation="MRIFVYGSLRHKQGNSHWMTNAQLLGDFSIDNYQLYSLGHYPGA
                     VPGNGTVHGEVYRIDNATLAELDALRTRGGEYARQLIQTPYGSAWMYVYQRPVDGLKL
                     IESGDWLDRDK"
     gene            25994..27466
                     /locus_tag="J1Y83_000467"
     CDS             25994..27466
                     /locus_tag="J1Y83_000467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000749210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sel1 repeat family protein"
                     /protein_id="HBA7628653.1"
                     /translation="MKKSLLAVMLTGLFALVSLPALGNVNLEQLKQKAESGEAKAQLE
                     LGYRYFQGNETTKDLTQAMDWFRRAAEQGYTPAEYVLGLRYMNGEGVPQDYAQAVIWY
                     KKAALKGLPQAQQNLGVMYHEGNGVKVDKAESVKWFRLAVEQGRDSGQQSMGDAYFEG
                     DGVTRDYVMAREWYSKAAEQGNVWSCNQLGYMYSRGLGVERNDAISAQWYRKSATSGD
                     ELGQLHLADMYYFGIGVTQDYTQSRVLFSQSAEQGNSIAQFRLGYILEQGLAGAKEPL
                     KALEWYRKSAEQGNSDGQYYLAHLYDKGAEGVAKNREQAISWYTKSAEQGDATAQANL
                     GAIYFRLGSEEEHKKAVEWFRKAAAKGEKAAQFNLGNALLQGKGVKKDEQQAAIWMRK
                     AAEQGLSAAQVQLGEIYYYGLGVERDYVQAWAWFDTASTNDMNLFGTENRNITEKKLT
                     AKQLQQAELLSQQYIEKYAPEAWARMQKLKAQSAVKTGNK"
     gene            complement(27880..28410)
                     /gene="ppa"
                     /locus_tag="J1Y83_000468"
     CDS             complement(27880..28410)
                     /gene="ppa"
                     /locus_tag="J1Y83_000468"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001739810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HBA7628654.1"
                     /translation="MSLLNVPAGKDLPEDIYVVIEIPANADPIKYEIDKESGALFVDR
                     FMSTAMFYPCNYGYINHTLSLDGDPVDVLVPTPYPLQPGSVIRCRPVGVLKMTDEAGE
                     DAKLIAVPHTKLSKEYDHIKDVNDLPELLKAQIAHFFEHYKDLEKGKWVKVEGWENAE
                     AAKAEIVASFERAKNK"
     gene            28720..29676
                     /gene="ytfQ"
                     /locus_tag="J1Y83_000469"
     CDS             28720..29676
                     /gene="ytfQ"
                     /locus_tag="J1Y83_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter
                     substrate-binding protein YtfQ"
                     /protein_id="HBA7628655.1"
                     /translation="MWKRLLVVSAVSAAMSSMALAAPLTVGFSQVGSESGWRAAETNV
                     AKSEAEKRGITLKIADGQQKQENQIKAVRSFVAQGVDAIFIAPVVATGWEPVLKEAKD
                     AEILVFLLDRSIDVKDKSLYMTTVTADNILEGKLIGDWLVKEVNGKPCNVVELQGTVG
                     ASVAIDRKKGFAEAIKNAPNIKIIRSQSGDFTRSKGKEVMESFIKAENNGKNICMVYA
                     HNDDMVIGAIQAIKEAGLKPGKDILTGSIDGVPDIYKAMIDGEANASVELTPNMAGPA
                     FDALEKYKKDGTMPEKLTLTKSTLYLPDTAKEELEKKKNMGY"
     gene            29816..31318
                     /locus_tag="J1Y83_000470"
     CDS             29816..31318
                     /locus_tag="J1Y83_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HBA7628656.1"
                     /translation="MTTDQHQEILRTEGLSKFFPGVKALDNVDFSLRRGEIMALLGEN
                     GAGKSTLIKALTGVYHADRGTIWLEGQAISPKNTAHAQQLGIGTVYQEVNLLPNMSVA
                     DNLFIGREPKRFGLLRRKEMEKRATELMASYGFSLDVREPLNRFSVAMQQIVAICRAI
                     DLSAKVLILDEPTASLDTQEVELLFGLMRQLRDRGVSLIFVTHFLDQVYQVSDRITVL
                     RNGSFVGCRETRELPQIELVKMMLGRELDTHALQRAGRTLLSDKPVAAFKNYGKKGTI
                     APFDLEVRPGEIVGLAGLLGSGRTETAEVIFGIKPADSGTALIKGKLQTLRSPHQASV
                     LGIGFCPEDRKTDGIIAAASVRENIILALQAQRGWLRPISRKEQQEIAERFIRQLGIR
                     TPSTEQPIEFLSGGNQQKVLLSRWLLTRPQFLILDEPTRGIDVGAHAEIIRLIETLCA
                     DGLALLVISSELEELVGYADRVIIMRDRKQVAEIPLAELSVPAIMNAIAA"
     gene            31332..32354
                     /locus_tag="J1Y83_000471"
     CDS             31332..32354
                     /locus_tag="J1Y83_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBA7628657.1"
                     /translation="MPQSLPDTTPPKRRFRWPTGMPQLAALLLVLLVDSLVAPHFWQV
                     VLQDGRLFGSPIDIFNRAAPVALLAIGMTLVIATGGIDLSVGAVMAIAGATTAAMTVA
                     GFSLPIVLLSALGTGILAGLWNGILVAILKIQPFVATLILMVAGRGVAQLITSGQIVT
                     FNSPDLSWFGSGSLLFLPTPVIIAVLTLLLFWLLTRKTALGMFIEAVGINIRAAKNAG
                     VNTRIIVMLTYVLSGLCAAIAGIIVAADIRGADANNAGLWLELDAILAVVIGGGSLMG
                     GRFNLLLSVVGALIIQGMNTGILLSGFPPEMNQVVKAVVVLCVLIVQSQRFISLIKGG
                     RNRDKT"
     gene            32341..33336
                     /gene="yjfF"
                     /locus_tag="J1Y83_000472"
     CDS             32341..33336
                     /gene="yjfF"
                     /locus_tag="J1Y83_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease YjfF"
                     /protein_id="HBA7628658.1"
                     /translation="MIKRNLPLMITIGVFVLGYIYCLTQFPGFASTRVICNILTDNAF
                     LGIIAVGMTFVILSGGIDLSVGSVIAFTGVFLAKVIGDFGLSPLLAFPLVLVMGCAFG
                     AFMGLLIDALKIPAFIITLAGMFFLRGVSYLVSEESIPINHPVYDTLSSLAWKIPGGG
                     RLSAMGLLMLAVVVIGILLAHRTRFGNQVYAIGGNATSANLMGISTRSTTIRIYMLST
                     GLATLAGIVFSIYTQAGYALAGVGVELDAIASVVIGGTLLSGGVGTVLGTLFGVAIQG
                     LIQTYINFDGTLSSWWTKIAIGILLFIFIALQRGLTVLWENRQSSPVTRVNIAQR"
     gene            complement(33369..34367)
                     /gene="fbp"
                     /locus_tag="J1Y83_000473"
     CDS             complement(33369..34367)
                     /gene="fbp"
                     /locus_tag="J1Y83_000473"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 fructose-bisphosphatase"
                     /protein_id="HBA7628659.1"
                     /translation="MKTLGEFIVEKQHEFSHATGELTALLSAIKLGAKIIHRDINKAG
                     LVDILGASGAENVQGEVQQKLDLFANEKLKAALKARDIVAGIASEEEDEIVVFEGCEH
                     AKYVVLMDPLDGSSNIDVNVSVGTIFSIYRRVTPVGTPVTEEDFLQPGNKQVAAGYVV
                     YGSSTMLVYTTGCGVHAFTYDPSLGVFCLCQERMRFPEKGKTYSINEGNYIKFPNGVK
                     KYIKFCQEEDKSTNRPYTSRYIGSLVADFHRNLLKGGIYLYPSTASHPDGKLRLLYEC
                     NPMAFLAEQAGGKASDGKERILDIIPETLHQRRSFFVGNDHMVEDVERFIREFPDA"
     gene            34543..35916
                     /gene="mpl"
                     /locus_tag="J1Y83_000474"
     CDS             34543..35916
                     /gene="mpl"
                     /locus_tag="J1Y83_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl-
                     meso-diaminopimelate ligase"
                     /protein_id="HBA7628660.1"
                     /translation="MRIHILGICGTFMGGLAMLARQLGHEVTGSDANVYPPMSTLLEK
                     QGIELIQGYDASQLDPQPDLVIIGNAMTRGNPCVEAVLEKNIPYMSGPQWLHDFVLRD
                     RWVLAVAGTHGKTTTAGMATWILEQCGYKPGFVIGGVPGNFEVSARLGESDFFVIEAD
                     EYDCAFFDKRSKFVHYCPRTLILNNLEFDHADIFDDLKAIQKQFHHLVRIVPGQGRII
                     WPENDINLKQTMAMGCWSEQELVGEQGHWQAKKLTTDASEWEVLLDGEKVGEVKWSLV
                     GEHNMHNGLMAIAAARHVGVAPADAANALGSFINARRRLELRGEANGVTVYDDFAHHP
                     TAILATLAALRGKVGGTARIIAVLEPRSNTMKMGICKDDLAPSLGRADEVFLLQPAHI
                     PWQVAEVAEACVQPAHWSGDVDTLADMVVKTAQPGDHILVMSNGGFGGIHQKLLDGLA
                     KKAEAAQ"
     gene            complement(36074..36625)
                     /locus_tag="J1Y83_000475"
     CDS             complement(36074..36625)
                     /locus_tag="J1Y83_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated protein"
                     /protein_id="HBA7628661.1"
                     /translation="MTKQPEDWLDDVPGDDIEDEDDEIIWVSKSEIKRDAEELKRLGA
                     EIVDLGKNALDKIPLDADLRAAIELAQRIKMEGRRRQLQLIGKMLRQRDVEPIRQALD
                     KLKNRHNQQVVLFHKLENLRDRLIDQGDDAIAEVLNLWPDADRQQLRTLIRNAKKEKE
                     GNKPPKSARQIFQYLRELAENEG"
     gene            36719..38071
                     /gene="pmbA"
                     /locus_tag="J1Y83_000476"
     CDS             36719..38071
                     /gene="pmbA"
                     /locus_tag="J1Y83_000476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease PmbA"
                     /protein_id="HBA7628662.1"
                     /translation="MALAMKVISQVEAQRKILEEAVSTALELASGKSDGAEVAVSKTT
                     GISVSTRYGEVENVEFNSDGALGITVYHQNRKGSASSTDLSPQAIARTVQAALDIARY
                     TSPDPYAGVADKELLAFDAPDLDLFHPADVSPDEAIELAARAEQAALQADKRITNTEG
                     GSFNSHYGVKVFGNSHGMLQGYCSTRHSLSSCVIAEENGDMERDYAYTIGRAMSDLQT
                     PEWVGADCARRTLSRLSPRKLSTMKAPVIFANEVATGLFGHLVGAIAGGAVYRKSTFL
                     LDSLGKQILPDWLTIEEHPHLLKGLASTPFDSEGVRTERRDIVKDGILTQWLLTSYSA
                     RKLGLKSTGHAGGIHNWRIAGQGLSFEQMLKEMGTGLVVTELMGQGVSAITGDYSRGA
                     AGFWVENGEIQYPVSEITIAGNLKDMWRNIVTVGNDIETRSNIQCGSVLLPEMKIAGQ
                     "
     gene            38126..38512
                     /gene="cybC"
                     /locus_tag="J1Y83_000477"
     CDS             38126..38512
                     /gene="cybC"
                     /locus_tag="J1Y83_000477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313240.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b562"
                     /protein_id="HBA7628663.1"
                     /translation="MRKSLLAILAVSSLVFSSASFAADLEDNMETLNDNLKVVEKADN
                     AVQVKDALTKMRAAALDAQKATPPKLEGKSPDSPEMKDFRHGFDILVGQIDDALKLAN
                     EGKVKEAQAAAEQLKTTRNAYHQKYR"
     gene            complement(38557..39021)
                     /gene="nrdG"
                     /locus_tag="J1Y83_000478"
     CDS             complement(38557..39021)
                     /gene="nrdG"
                     /locus_tag="J1Y83_000478"
                     /EC_number="1.97.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate
                     reductase-activating protein"
                     /protein_id="HBA7628664.1"
                     /translation="MNYHQYYPVDIVNGPGTRCTLFVSGCVHECPGCYNKSTWRVNSG
                     QPFTKAMEDQIINDLNDTRIKRQGISLSGGDPLHPQNVPDILKLVQRIRAECPGKDIW
                     VWTGYKLDELNAAQMQVVDLINVLVDGKFVQDLKDPSLIWRGSSNQVVHHLR"
     gene            complement(39179..41317)
                     /gene="nrdD"
                     /locus_tag="J1Y83_000479"
     CDS             complement(39179..41317)
                     /gene="nrdD"
                     /locus_tag="J1Y83_000479"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004128226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HBA7628665.1"
                     /translation="MTPHVMKRDGCKVPFKSERIKEAILRAAKAAEVDDADYCATVAA
                     VVSEQMQGRSQVDINEIQTAVENQLMSGPYKQLARAYIEYRHDRDIEREKRGRLNQEI
                     RGLVEQTNASLLNENANKDSKVIPTQRDLLAGIVAKHYARQHLLPRDVVQAHERGDIH
                     YHDLDYSPFFPMFNCMLIDLKGMLTQGFKMGNAEIEPPKSISTATAVTAQIIAQVASH
                     IYGGTTINRIDEVLAPFVTASYNKHRKTAEEWNIPDAEGYANSRTIKECYDAFQSLEY
                     EVNTLHTANGQTPFVTFGFGLGTSWESRLIQESILRNRIAGLGKNRKTAVFPKLVFAI
                     RDGLNHKKGDPNYDIKQLALECASKRMYPDILNYDQVVKVTGSFKTPMGCRSFLGVWE
                     NENGEQIHDGRNNLGVISLNLPRIALEAKGDEATFWKLLDERLVLARKALMTRIARLE
                     GVKARVAPILYMEGACGVRLNADDDVSEIFKNGRASISLGYIGIHETINALFGGEHVY
                     DNEQLRAKGIAIVERLRQAVDQWKEETGYGFSLYSTPSENLCDRFCRLDTAEFGVVPG
                     VTDKGYYTNSFHLDVEKKVNPYDKIDFEAPYPPLANGGFICYGEYPNIQHNLKALEDV
                     WDYSYQHVPYYGTNTPIDECYECGFTGEFECTSKGFTCPKCGNHDTSRVSVTRRVCGY
                     LGSPDARPFNAGKQEEVKRRVKHLGNGQIG"
     gene            complement(41711..43366)
                     /gene="treC"
                     /locus_tag="J1Y83_000480"
     CDS             complement(41711..43366)
                     /gene="treC"
                     /locus_tag="J1Y83_000480"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HBA7628666.1"
                     /translation="MTHLPHWWQNGVIYQIYPKSFQDTTGSGTGDLRGVIQRLDYLHK
                     LGVDAIWLTPFYVSPQVDNGYDVANYTAIDPTYGTLDDFDELVTQAKSRGIRIILDMV
                     FNHTSTQHAWFREALNKESPYRQFYIWRDGEPETPPNNWRSKFGGSAWRWHAESEQYY
                     LHLFAPEQADLNWENPAVRAELKKVCEFWADRGVDGLRLDVVNLISKDPRYPEDLDGD
                     GRRFYTDGPRAHEFLHEMNRDVFTPRGLMTVGEMSSTSLEHCQRYAALTGSELSMTFN
                     FHHLKVDYHGGEKWTLAKPDFVALKTLFRHWQQGMHNVAWNALFWCNHDQPRIVSRFG
                     DEGEYRVPAAKMLAMVLHGMQGTPYIYQGEEIGMTNPHFTRITDYRDVESLNMFAELR
                     NDGRDADELLTILASKSRDNSRTPMQWTNGDNAGFTAGEPWIGLGDNYQEINVEAALA
                     DESSVFYTYQKLIALRKQEAVLTWGDYQDLLPNSPVLWCYRREWKGQTLLVIANLSRE
                     TQPWQPGKMLGNWQLVMHNYEEASPQPCTMTLRPFEAVWWLQK"
     gene            complement(43416..44837)
                     /gene="treB"
                     /locus_tag="J1Y83_000481"
     CDS             complement(43416..44837)
                     /gene="treB"
                     /locus_tag="J1Y83_000481"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS trehalose transporter subunit IIBC"
                     /protein_id="HBA7628667.1"
                     /translation="MMSKINQTDIDRLIELVGGRGNIATVSHCITRLRFVLNQPANAR
                     PKEIEQLPMVKGCFTNAGQFQVVIGTNVGDYYQALIASTGQAQVDKEQVKKAARQNMK
                     WHEQLISHFAEIFFPLLPALISGGLILGFRNVIGDLPMSNGQTLAQMYPSLQTIYDFL
                     WLIGEAIFFYLPVGICWSAVKKMGGTPILGIVLGVTLVSPQLMNAYLLGQQLPEVWDF
                     GMFSIAKVGYQAQVIPALLAGLALGVIETRLKRIVPDYLYLVVVPVCSLILAVFLAHA
                     LIGPFGRMIGDGVAFAVRHLMTGSFAPIGAALFGFLYAPLVITGVHQTTLAIDLQMIQ
                     SMGGTPVWPLIALSNIAQGSAVIGIIISSRKHNEREISVPAAISAWLGVTEPAMYGIN
                     LKYRFPMLCAMIGSGLAGLLCGLNGVMANGIGVGGLPGILSIQPSYWQVFALAMAIAI
                     IIPIVLTSFIYQRKYRLGTLDIV"
     gene            complement(44956..45903)
                     /gene="treR"
                     /locus_tag="J1Y83_000482"
     CDS             complement(44956..45903)
                     /gene="treR"
                     /locus_tag="J1Y83_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator TreR"
                     /protein_id="HBA7628668.1"
                     /translation="MQNRLTIKDIARLSGVGKSTVSRVLNNESGVSQRTRERVEAVMN
                     QHGFSPSRSARAMRGQSDKVVAIIVTRLDSLSENLAVQTMLPAFYEQGYDPIMMESQF
                     SPQLVAEHLGVLKRRNIDGVVLFGFTGITEEMLAHWQSSLVLLARDAKGFASVCYDDE
                     GAIKILMQRLYDQGHRNISYLGVPHSDVTTGKRRHEAYLAFCKTHKLHPVAALPGLAM
                     KQGYENVAKVITPETTALLCATDTLALGASKYLQEQRIDTLQLASVGNTPLMKFLHPE
                     IVTVDPGYAEAGRQAACQLIAQVTGRSEPQQIIIPATLS"
     gene            46088..46141
                     /gene="mgtL"
                     /locus_tag="J1Y83_000483"
     CDS             46088..46141
                     /gene="mgtL"
                     /locus_tag="J1Y83_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_003933616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mgtA regulatory leader peptide MgtL"
                     /protein_id="HBA7628669.1"
                     /translation="MEPDPTPLPRRRLKLFR"
     gene            46282..48978
                     /gene="mgtA"
                     /locus_tag="J1Y83_000484"
     CDS             46282..48978
                     /gene="mgtA"
                     /locus_tag="J1Y83_000484"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HBA7628670.1"
                     /translation="MFKEIFTRLIRHLPSRLVHRDPLPGAQQTVNAAVPPSLSAHCLK
                     MAVMPEEELWKTFDTHPEGLNLAEVESAREQHGENKLPAQQPSPWWVHLWVCYRNPFN
                     ILLTILGAISYATEDLFAAGVIALMVAISTLLNFIQEARSTKAADALKAMVSNTATVL
                     RVINDKGENGWLEIPIDQLVPGDIIKLAAGDMIPADLRILQARDLFVAQASLTGESLP
                     VEKAATTRQPEHSNPLECDTLCFMGTTVVSGTAQAMVIATGANTWFGQLAGRVSEQES
                     EPNAFQQGISRVSMLLIRFMLVMAPVVLLINGYTKGDWWEAALFALSVAVGLTPEMLP
                     MIVTSTLARGAVKLSKQKVIVKHLDAIQNFGAMDILCTDKTGTLTQDKIVLENHTDIS
                     GKTSERVLHSAWLNSHYQTGLKNLLDTAVLEGTDEESARSLASRWQKIDEIPFDFERR
                     RMSVVVAENTEHHQLVCKGALQEILNVCSQVRHNGEIVPLDDTMLRKIKRVTDTLNRQ
                     GLRVVAVATKYLPAREGDYQRADESDLILEGYIAFLDPPKETTAPALKALKASGITVK
                     ILTGDSELVAAKVCHEVGLDAGEVVIGSDIETLSDDELANLAQRTTLFARLTPMHKER
                     IVTLLKREGHVVGFMGDGINDAPALRAADIGISVDGAVDIAREAADIILLEKSLMVLE
                     EGVIEGRRTFANMLKYIKMTASSNFGNVFSVLVASAFLPFLPMLPLHLLIQNLLYDVS
                     QVAIPFDNVDDEQIQKPQRWNPADLGRFMVFFGPISSIFDILTFCLMWWVFHANTPET
                     QTLFQSGWFVVGLLSQTLIVHMIRTRRVPFIQSCASWPLMIMTVIVMIVGIALPFSPL
                     ASYLQLQALPLSYFPWLVAILAGYMTLTQLVKGFYSRRYGWQ"
     gene            complement(49184..49570)
                     /gene="ridA"
                     /locus_tag="J1Y83_000485"
     CDS             complement(49184..49570)
                     /gene="ridA"
                     /locus_tag="J1Y83_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminobutanoate/2-iminopropanoate deaminase"
                     /protein_id="HBA7628671.1"
                     /translation="MSKTIATENAPAAIGPYVQGVDLGNMIITSGQIPVNPKTGEVPA
                     DVAAQARQSLDNVKAIVEAAGLKVGDIVKTTVFVKDLNDFATVNATYEAFFTEHNATF
                     PARSCVEVARLPKDVKIEIEAIAVRR"
     gene            complement(49643..50104)
                     /gene="pyrI"
                     /locus_tag="J1Y83_000486"
     CDS             complement(49643..50104)
                     /gene="pyrI"
                     /locus_tag="J1Y83_000486"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000148566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase regulatory
                     subunit"
                     /protein_id="HBA7628672.1"
                     /translation="MTHDNKLQVEAIKRGTVIDHIPAQIGFKLLSLFKLTETDQRITI
                     GLNLPSGEMGRKDLIKIENTFLSEDQVDQLALYAPQATVNRIDNYEVVGKSRPSLPER
                     IDNVLVCPNSNCISHAEPVSSSFAVRKRANDIALKCKYCEKEFSHNVVLAN"
     gene            complement(50117..51052)
                     /gene="pyrB"
                     /locus_tag="J1Y83_000487"
     CDS             complement(50117..51052)
                     /gene="pyrB"
                     /locus_tag="J1Y83_000487"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase"
                     /protein_id="HBA7628673.1"
                     /translation="MANPLYQKHIISINDLSRDDLNLVLATAAKLKANPQPELLKHKV
                     IASCFFEASTRTRLSFETSMHRLGASVVGFSDSANTSLGKKGETLADTISVISTYVDA
                     IVMRHPQEGAARLATEFSGNVPVLNAGDGSNQHPTQTLLDLFTIQETQGRLDNLHVAM
                     VGDLKYGRTVHSLTQALAKFDGNRFYFIAPDALAMPQYILDMLDEKGIAWSLHSSIEE
                     VMAEVDILYMTRVQKERLDPSEYANVKAQFVLRASDLHNAKANMKVLHPLPRVDEIAT
                     DVDKTPHAWYFQQAGNGIFARQALLALVLNRDLVL"
     gene            complement(51056..51190)
                     /gene="pyrL"
                     /locus_tag="J1Y83_000488"
     CDS             complement(51056..51190)
                     /gene="pyrL"
                     /locus_tag="J1Y83_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyr operon leader peptide"
                     /protein_id="HBA7628674.1"
                     /translation="MVQCVRHFVLPRLKKDAGLPFFFPLITHSQPLNRGAFFCPGVRR
                     "
BASE COUNT        12250 a        12934 c        13654 g        12468 t
ORIGIN      
        1 tcaggtacag aggaaatatt gaaaattatt gtgtgtaaat gtctcccttt aaaaagtctg
       61 gccagttttt ttcaaagtaa ataccgttaa tacacggttg catacgtaaa gactttactt
      121 cggtaataaa agaaccttgc gaatattgaa ttgttaattt gtgatttttg tcttcagcga
      181 gttcagagcg aaaacgccat tcttctttta cgccgcgacg agtttgtggt aggcatttgc
      241 cttgtggatc aataaccatc cgcgccccac gactgccgcc acctgcgtca atataatgcg
      301 ttaattgcgt taatacggcg gcagaagtca gtgccatatg ccgccacata aacagctcgg
      361 caacttcacc cacatgtttg atagccaatc catgccgttg gacaaattcg ttcaataaca
      421 gggcatcacg agtggcacgt cggactttat cggcatggca aataaatccc gcatggtcag
      481 acattcgtgc ctgaattttt tctctcacaa ccgataacgg catcccggtt ccgttatcgg
      541 gcgcctgagt aatgatctct cttatagaag ccagtgccgg agctgccagc tgtgctatat
      601 ctccatcaat attccgcttt tgcgtgcaac caataaaacg tgccagacga acagcaaaaa
      661 cctgcccggc attcaacgcc gcgccgccag gtcgggtgac gccgtgtgtg ccagcaactt
      721 cccccactgc aaaacaaccg ggcagagatg tttgtcccca gatatcgact tcaatgccgc
      781 cattcatatg ctgattattc atggcaaact gcaatggctg cgtggtgaga tcgtaaccgt
      841 gcatcttata cagcgagata gacagcggat tcattcgttg cagtcgttcg atgggcgatg
      901 gtgccagagc gtcgttattt tccagatacg cgcgaacgtc gtcgtccagt cgatctaatg
      961 agaagggcaa atcacccggc accacttcag gattacgatt gaaatccata aataccagac
     1021 gccctgattg ctgctcttgc gccaccgcca tatctaacaa actggagcca aaatccatta
     1081 cccgcgtggc gtggaacggc cactgatagc ctttacggaa aatgtttgaa gctagctcct
     1141 gggtggtgcg atagtaattc gcgaggaagt tatactcgtt accctcagca tctacggaat
     1201 agatatacgg gatcacctgc acataggtgc cggacaaatt ccacggaaac gtgctacgcg
     1261 gcgtgcctat accaaactgg ctttcggtca gattggttag cgtcaggcct tcctccagcg
     1321 ccagccccag cgagccaaaa catttgtgtg ggtacacact gtcgcgataa agctcgccag
     1381 gccctcctgt tgccagtacc acattgggcg cagtcacaat tgccagcccc catgggttat
     1441 gggcgcgatg accggtcgcg aggattgccc ccgccacgcg gtcttcgccg ttttcgtcac
     1501 gctgatgcag cagtttaatc actgttgcac tggtcaacat tgggatagca agacgctgta
     1561 cttcttccag cagcactttc accattaacc ttgaggtacg cggcccacac gaggttgcac
     1621 gcccggcttc gtcgtggtcg gtttgataac gaagaatcgc gccatagcga tcttccggta
     1681 attccagacc aagatattgc aacccgccaa gagtgtgcag agaacctacc gcttcaacat
     1741 aagcggtgtc gtgatccatc gccccaccgc tcgccagtgc ctctgccagt ttggcgaagt
     1801 tgtcgccgtt gcccgcggta gcggcggtaa acagtgtttg tttatcagag ccagaacatg
     1861 ccgatgtccc catatataac ccggcggtgg cgattaacac attttgctgg cggcgtttaa
     1921 gttccacagc tgcacgtagc ccggctgcac cactgcctac taccagggca tctgttttcc
     1981 agagtgggta atcaacaccg tctaccgtga caacccatgc gggttctgcc tgtgcaaaac
     2041 cctttgggaa agtaacgtgt gtcagtgcat caaggatggc ctcatcaagc tggctcatgc
     2101 atcctccatc ggcgtaaaca ggctactttc catcaggcgt gggtgcttga taattggacg
     2161 gaaggccatg aatggcagaa tgtcgcgctc aatctctacg ccaggggcga tctcaattaa
     2221 ttcaacgcct tctgccgtta actggaatac cgcacgttcg gtgatataca gcacttcctg
     2281 accgctttgc tgtgcgtatg aggcgctaaa ggtgattttt tccaccaggg taaccagttt
     2341 agggatttga cctgactggg cgatatgcag gccatcaggc gttacatcaa tcttgctgcc
     2401 tttggcgtca aacgtaccgc agaacaccac tttacgggcg ttttgcgcaa tttcgaggaa
     2461 gccgccgggg ccaatgagat tgccattgag atgggatacg ttgacgttac cgtaccgatc
     2521 catctctccc atgccgagga aggtgatatc gataccgccg ccactgtaga attcgaattg
     2581 atcgagtgat ggaataatgg catcggcgtt acgggcgcag ccgaacagag catcatccag
     2641 catcatgccg ttatgtacac cctgttctac actcagccac aaactttgat aaggcacgcc
     2701 ttcgcgcagt gcaaccccag gaataccgcc gggaatacca aagccaaagt tggttgaagc
     2761 gcctgccacc agttcccgcg ctgcccgacg ggcaatcagg cgacgcaccg gatgactaac
     2821 cagttcaata gcgtcgttag aacttagacg gcgatgttga ccgctgatcg tcaggtcgta
     2881 accaccaaga taggtttgcg gttgttcgcg gtgctcaacg atgccatcga ccagaattcc
     2941 tggtagcttg acccgacgtg gttcaatggt acctgcttcc agcacatcgc ggacctgtac
     3001 gaacactttg ccgccgctgt tgtgtgctgc cagcgccatc gaatagctat ccatatcaat
     3061 cgcttcttct tcgaggctaa cgttgcctcg tggatcggca taagtgccac gcaaaatcgc
     3121 gtaatccact ttgaaagggc gataacgaag tttggtctca ccgtcgattt caatcagttc
     3181 taccaggtcg tcggtcgtgc attcattcga cttaccgcca ccattgcgtg gatcaacaaa
     3241 cgatcccagc ccaacgtggg taaaaagccc cggacgtccg gcaccgatct cccgcagcag
     3301 tgcctgaatt acgccaccag ggaaacagta ggcttcaata gcattgcttt tcaccagttc
     3361 ctgcatcttg ggcgaccagg taaaatgtcc ggcaataatg cgtttgagca tttcggcgtg
     3421 agcaaaacgg ttagtgcctt tgctgtcgcg atcgccaata cccagcgagt ggattaatgt
     3481 cagatcgcgc gggtgtccgg tttgcaggaa acgctcttca atggcggcca taatatggtc
     3541 agcttccacc ataccgccgc cgttaccgct aatggcaatg gttgcgccgt cctgaatttg
     3601 cgtcgccagt gtctcggcgg ttgataactt acgcatacaa tttccttatt gattgtggaa
     3661 gctcacgccg gctttttcgg caaatgcgtg cgtcgcctgt tggcaatcct gagtggcgga
     3721 cattaatcca gccagtaact ggtgggagta atcggcgtgt ttattttcca gcgccagaat
     3781 cagttgctta atatgtgcca gtgccaccgg accacatttt tccagctgcg ctaactggtt
     3841 cgccatccag ctttccacct gttctttttc taccacttca tcaattaaat tcgccacctg
     3901 ggcttcctgc gccgtcaggc gtttacccag catcagcatc tggcgaccga ctaccgggcc
     3961 aacctgatta agcacgcgtt caatgcccat ccagccaggc accatgccga gcatcacttc
     4021 cgggttagag aatttcgcgc cggggcgagc gatacgaata tcgcagcaca aggcaagttc
     4081 aataccgcca ccgatggtat gaccgttgag attcgcaacg gttaattggg gtaagttgcg
     4141 caggcgatta aaaacgtcat taccgcgttt aatccatttt ctgcccatat ctaatggcga
     4201 ataggccgac cacgatttaa tatcgccgcc cgcacaaaag aatttatcgc cggttgccgt
     4261 taattcgact aagcgtaatg ccgtgtcatt ttcaatttca tttaaataat ggtcgagaga
     4321 ttcgatcatt tcttcattaa tagcatgaca tttatcttca cggttcaatg tcaggcggca
     4381 agaagccgcc gctctgctga acagaacggg ttggtcactc atgattattc cttaacaata
     4441 atgtgcgatg cgatcgacgc taatatccag cgtgctgtta atatctttct gccaccagta
     4501 cggtgagaat atttccagtt caatagcgcc attaaatccg gcgttttcaa ccagccgacg
     4561 aattgacgga atattgatga caccatctcc cggcattcca cggtcattga ccagatcggt
     4621 ggtcggcact aaccagtcgg aaacatgaaa cgcgaggatg cgttttccgg cacgcaggat
     4681 ctgactggcg agatccgggt cccaccagac atggtagaca tctaccgcca cgccgaggcc
     4741 aaattcgcca tcgggatcga gttcatcaca ccagtccagc gcctgacgta acgtgcacaa
     4801 acaggaacga tcggctgcgg tcatcgggtg tagcggctcc agcgcaatcg gcacgccgac
     4861 ctctttcgaa tgtggcagta actggcgaat cccttgtttc acttgttccc gcgcttcata
     4921 cagatctttt gttcccattg gcagaccgcc aaccacctgc atataacagg cggcgttcag
     4981 tacagcggcg tcgtccagcg cccggcggtt atcgtcaatc gccagtttgc gctccgccag
     5041 cgtcggtgcg gtgtagtagg tgctacggca caaaccggaa acgtttatgt tactggcagc
     5101 aagctggcgg gcgatttgct ggagatcttc accctgcaac tccctgcgcc agggggcaat
     5161 ggcgccaata ccgcgtgcgg cgcaggcatc aataatggcg ggcagcggcg ttttatagcc
     5221 cagtgtcgcg gtattgatac tgaataaatc cgggcgttgt aacagatctc tcatgattca
     5281 gcctcagaag cgcggcatac ttaagccgcc gtcgatggcg accgcctgac cacaggtgta
     5341 aatcagttta ccttccgcca tcgcgcggac agtggaagca atatcagccg gataacccca
     5401 acgtccccac ggcaccagac ctttggcaat cagttcgtcg taataggccg ttgccggaat
     5461 agtcatatcg gttttgatga gaccaggacg cacttcataa acgccaattt gttcattaca
     5521 aaggcgagca gcaaacagac gagcagcggc agagacggca gttttggcaa tggtatattc
     5581 gccgcgattc atcgccagca tgatggcgtt gatggaactg acgaaaataa tcgagcggtg
     5641 cggcagttct gcttccggtt tcggctgtgc cagtaagcgc ttactgaatg cctgagcgag
     5701 gaaaaacggt gcgcgggtgt taatggcaat attttggtcg aagctgtctg gttcgaggtc
     5761 gagaaggtca ccgcgttttt tcactgaaat gcctgcgttg ttgagcaggc agtccagacg
     5821 tccccactgg ttttgcgctg cgtcgagcat ctcttcatgc agggagagat cgcccacatc
     5881 agcagggaag caaatcactt ccacgccttc cgccagacat tcttgttgtg tggcatgtaa
     5941 tttttctacg ctatcagcat ccggacgatc attaatcagc agattaaatc cgccacgcgc
     6001 aagctccagc gcacagccct ttccaatacc acgtgcggct ccggtaatca cggcaacagg
     6061 acgagtggat ttcatacaat agctctcttt ttttgaattt tgtttcctta gtgatgatta
     6121 gcttgcgact ggaggataaa aagatcaacc atctagttgg ttgatatgcg atggcgatca
     6181 caggacattt gataacctga tgaaagtaca agtttttgcc cttttttctg aagctgaaat
     6241 caaatcaggt tatgatagtc accacgaaga ggtcgtgggg aagcgttatg tatcttgata
     6301 ccagcaacgt acagagcctg aaagaaaagc tgctgttatg cgcggttaat gaatttgccg
     6361 aatatggtta cgaaggggcc agagtcgata atatcgtcaa ggccgcgggg tgcagtaaac
     6421 agacggttta tcaccacttt ggtaataaag aaaatttatt tatcgaagta ctggaatata
     6481 cctggaacga tattcgacaa aaagaaaagg cccttgattt ctcagatctt ccgccacaga
     6541 aagccattga gaaaataatc gattttacat ggggttatta tatttctaat ccctggtttt
     6601 taaaaattgt gcatagtgaa aatcaaagta agggtgtgca ttatgcaaaa tcacagcgtc
     6661 ttttagaaat taaccatgcg cacttgcagt taatggaatc cttattggat gaagggaaga
     6721 aacaaaatat ttttaagcca gatatcgacc cattgcaggt gaatattaat attgctgcgc
     6781 ttgggggata ttatttgatc aaccagcata cgcttggcct ggtttatcac atcagtatgg
     6841 tttcaccgca ggcactggaa gccagacgta aggtcatcaa agagacaatc cttagctggc
     6901 ttttggttga cccttcatct accgcgcgcg aataaaactg ccatccggct ggcgggtaaa
     6961 caggacctgt tgattattcc ccgtatcaat ggttaagccc gtcaccacgc cgctggcgtt
     7021 ctggcggatt ttcaccatct gcccgttttg caaattactc agtggcttat ccgcgccttc
     7081 tacctgcgcc atggcataga catcggtagc gggcagtccg tggtcgcgga acagctgcgc
     7141 catcgtttta ccgggttcga cacgatagga gcgccattga ttatcaattc cgctatctgg
     7201 ctggaatggt tgcgtatggg tggtttgcgg ctgctcttct ggttggcctt cctgtattgg
     7261 ttctggagcg accggagcca cctgatcagg atcattttgt ggcgtgacta attgcgcccg
     7321 cagctgttct tctgtcggtg gttgtgattg cgactgaata tctaactgcg cttcacgcgt
     7381 caccaccggc gcgttgggcg tatcatcaga tggaagcaga aatccgacta ccaatacaat
     7441 ggcggcaata atgatccccc gacgatgcat cggcggcagc gggtccatga agcgaaaatt
     7501 gtccggcgcg tgccagactt tctccagggt tggttttagt tcaaagcgcc cgggcatggt
     7561 ttcctcctgc tccgcgtctt gttcctcaat catagcctat gaataagcta acgctatgat
     7621 gtccgtggta aacccgcctt tattatattc atacgggata ttgctattgt ttctttttcc
     7681 ccgggatttg tcatcattcc cgcgacaaag ttttacccaa agaagtgtgg ctgatatgct
     7741 gcccgctact ttatacccta agaaaggaaa tacgatgacc accccaactt ttgacaccat
     7801 cgaagcgcaa gcaagctacg gcattggttt gcaggtaggg caacaactga gtgaatctgg
     7861 cctggaaggg ctgctgccag aagcactggt tgcaggcatt gccgatgcgc tggaaggcaa
     7921 acatccggct gttccggttg atgtggtgca ccgcgcgctg cgtgaaatcc acgaacgcgc
     7981 cgatgccgtt cgtcgtcagc gtttccaggc gatggctgct gaaggtgtga aatacctgga
     8041 agaaaacgcc aaaaaagaag gtgtaaatag caccgaatct ggcctgcaat tccgcgtgat
     8101 caaccagggt gaaggcgcaa ttccggcacg taccgaccgc gttcgtgttc attacaccgg
     8161 aaaactgatc gacggcaccg tgtttgacag ctccgttgct cgcggcgagc cagctgaatt
     8221 cccggttaac ggtgtgatcc ctggctggat tgaagcactg actctgatgc cggtaggttc
     8281 taaatgggaa ctgactatcc cgcaggaact ggcatatggc gagcgtggcg ctggcgcgtc
     8341 catccctccg ttcagcaccc tggtgtttga agtcgaactg ctggaaatcc tctaagcagc
     8401 gcattctgtt cccctcgagc cagaggggag caggcattca gcaataaacc cttcagtttg
     8461 ccaaacggcg ctattttatg ttgcaaagac cctgtaagcg tgtatttttg tgagctgttt
     8521 cgcgttatca ccgtgatatg acactcactt taaacataaa attaacatta gatctaaatc
     8581 ttagtattca tcccgcgtat tgttacctaa tatcgatgag tcccgataca gattcgtcgt
     8641 atcatagact gactaaaggc cgtagagcct gaacaacaca gacaggtaca ggaagaaaaa
     8701 aacatggtag atcaggtaaa agtcgttgcc gatgatcagg ctccggctga acagtcgcta
     8761 cggcgcaatc tcacaaaccg acatattcag cttattgcca ttggcggtgc cattggtaca
     8821 gggttgttta tggggtccgg caaaacgatt agccttgccg ggccgtcgat cattttcgtt
     8881 tatatgatca tcggttttat gctctttttc gtgatgcggg caatggggga attgctgctt
     8941 tcgaatctgg aatacaaatc ttttagtgac ttcgcttccg atttactcgg gccgtgggca
     9001 ggatatttca ccggctggac ttactggttc tgctgggttg taaccggtat ggcagatgtg
     9061 gttgccatta ccgcctatgc gcaattctgg ttccctgggc tttctgactg ggttgcttcg
     9121 ttatccgtga tcattctgtt actggttcta aacctcgcca cggtaaaaat gttcggtgag
     9181 atggagttct ggtttgcgat gatcaaaatc gtcgccatcg tgtcgctgat tgttgtcggc
     9241 ctggtcatgg tggcgatgca ctttcagtca ccgaccggtg tggaagcatc atttgcacat
     9301 ttgtggaatg acggcggctg gttcccgaaa ggcttaagcg gcttctttgc cggattccag
     9361 atagcggttt tcgctttcgt ggggattgag ctggtaggta caacagctgc ggaaaccaaa
     9421 gatccggaga aatcactgcc acgcgcgatt aactctattc cgatccgtat cattatgttc
     9481 tacgtcttct cgctgattgt gattatgtcc gtgacgccgt ggagttcggt agtcccggag
     9541 aaaagcccgt tcgttgaact gtttgtgttg gtaggtttgc ctgcggctgc cagcgtgatc
     9601 aactttgtgg tgctgacctc tgcggcgtct tccgctaaca gcggtgtctt ctctaccagc
     9661 cgtatgctgt ttggcctggc ccaggaaggt gtggcaccga aagcgttcgc taaactctct
     9721 aagcgcgcag tacccgcgaa agggctgacc ttctcttgta tctgtctgct cggcggcgtg
     9781 gtgatgttgt atgtgaatcc cagcgtgatt ggcgcgttca cgatgattac aaccgtttcc
     9841 gcgattctgt ttatgtttgt ctggacgatt atcctttgct cgtacctggt gtatcgcaaa
     9901 cagcgtcctc atctgcatga gaagtcgatc tacaagatgc cactcggcaa gctgatgtgc
     9961 tgggtatgta tggcgttctt tgtgtttgtt ctggtgttgt tgacactgga agatgacacc
    10021 cgccaggcgc tgctggttac cccgctgtgg tttatcgcgc tggggctggg ctggctgttt
    10081 attggtaaga aacgggctgc tgaactgcgg aaataatcgc attatcatgc tggatggcgc
    10141 aatgccatcc agcttttaga tcactcaccc gccagcgcgc gcgggaatag tacattgttt
    10201 tccagactga tgtgctccat caggtcatca atcagttcat taatgccgtt atacatcgct
    10261 ttccaggtgg tgcaggcttc cggcggcggt gtgacgttat tggtggtgtg tttgatcact
    10321 tccagcagtt cgcccgcttc atcgtgctcg ctttccatta cgctgattgg ccccattgcc
    10381 tggctgccca tgccttgttt aatcatcggg aagagaattt gctcttcttt catcatatgg
    10441 ctggaaagct cttcatgcag catagtcagg tattttgtca gcccttttgg cacacttggt
    10501 ttgtcggcgt gaacgcgctc gactttagtc gcttgcagga tcagctccgg cagctgctcg
    10561 cgatggcgat cgtgatagcg cacaataatg tggtcgatga tttccgccag cggggcgcta
    10621 cgccagtctt tctcaatcgg ttgttcagca agctttgcca gttcggcttc aatgacgtca
    10681 acatccagtt ctttacgtgc cgccgcgcgc gccagcgtct gcttaccgcc acagcagtaa
    10741 tccatatcat atttacgaaa cagagccgaa gcgcgaggaa tagagagcgc cagttcacct
    10801 aaaggttggt cgcgataagc catagctgat acctcattct taataagata agatgcattt
    10861 taaatgcatc tttaaggcaa aagctataac ccttacgtag cagaggctta tttaactcac
    10921 tgccagcgtg tcaactttct gagacagatt cagttaaagg tttttctggt tttgctctga
    10981 ccgcaatgac cacgccaatc actaacagcg cgataccgct cagcgtcatt agcggcggca
    11041 tttgctggcg gagtaaaaag gtgtacagca aacctgccag cgtttcgaaa acaatcagcg
    11101 gcccgagaat cactgtcggt aatcgctggc tggcgacgtt ccagcagagt gcgccaaccc
    11161 atgagcaaag cacggctatc gcaaccatca gactaataaa caccagcgga cgggggccaa
    11221 agggtaagga gaagtccggc gtttgcatat tcagccagta acaggcgacg agatagccga
    11281 tgagagaaac cggcagcgtg accagcgcct gcgccgtcgc ccacatcatc ggatgtttgt
    11341 cgggattttc ccgcagccag cgggcgttgc gcagggcata ccatgcccag cagaccacgg
    11401 aaactaacgc cagcacgatg cctgaggtat aacgtgccca gtcaaaatcg gggagtccgt
    11461 ggtttaactc agcaatattc acacacgcca ggccgatgcc aatacaaacc agtgccgggg
    11521 cgagttttcc ccatgcgagt ttgccgtcgc gctggctata aagcagattg gcaaaaacgg
    11581 gaatcaccac cggcagggtg ccgataatca tcgtggaaac aggcgcgcca gtacgttgaa
    11641 tggcactggc aaggcagaag taatagataa ggttgcccat catggtgagc atcaaggcgg
    11701 taagccagtc gcgacgcgcc aactgacgca gacgcacgcg tcccagccag gcaatgggca
    11761 gcgcaattaa ccctagcgcc aggtaacgcc ccatcgactg taacatcgcc gggtattccg
    11821 gcacgatcaa cggcccgaca aaaataagcc cccacatcaa ccctgctaac agggcgtaca
    11881 gcacgccgct aatcatcact ggcatccact ggtctgtcag aagaaatatt caggaagcag
    11941 acatgattgc tcccggcatc ccgtcattat cagagaggat gcgggaggat gccactttaa
    12001 ttaactagtt attaacatta aaaagatggc ttacgctttc ggctaacgtt gtcgttgggc
    12061 ggccaatcaa tttgctaagc gttttgctgt catcaaacag accgcctttc gatgcgccaa
    12121 cgtcagaatc cgccagcata tccgccagtc catcaggcag tccgacgctt ttcagcgccg
    12181 cagcgaaatc ggcttcgctc agattttgat aggtaaccga ttttccgctc tgtttggtga
    12241 gttctgccgc taactgtgtc agcgtccagg cactatcgcc cgccagttcg taaaccttgc
    12301 cttcgtgacc ggcttcgcta attacgcgtg ccgcagctac cgcataatca gcccgcgttg
    12361 ctgaggcaat tttgccatcg cccgcggcac cgataaatac gccgtgttcc agtgctgccg
    12421 gggcgctggc gaggtagttt tcggtgtacc agccgttgcg cagcagggtg taaacgatgc
    12481 cagaatcagc cagcattttc tccgtctcga tgtgctcatc ggcgaggccg agcggggagg
    12541 aatcggcatg tagcaggctg gtataagcga taaatttcac gtcggccgcc tttgcggcat
    12601 taataacatt acgatgctgc ggggcacgtt gacccacttc gctggaagag atcagcagta
    12661 gtttttccac tccctgaagc gcagatgtca gtgcggcttc atcgccgtaa tcagcctgac
    12721 gcacggtaat gccttgtgct gtcagtgcct gtgcttttgc cggattacga acgatagcca
    12781 ctatttggct ggcaggaacc gttttcatca aggattcaat aacatagtga ccaagttggc
    12841 cagtggcacc agtaatagcg atcatgggaa gtctccatcg ttttgtcttg tgtttaaata
    12901 cgctaacact taaacttact tttagtaagt acgtacaaaa aggtaagtat gaaatgagtc
    12961 aggttagcct gtcgcaacaa ctgaaagagg gaaatctctt tgcggaacag tgcccgtcgc
    13021 gcgaggtgtt gaaacacgtc accagccgtt ggggggtgtt gattctggtg gcgctacgcg
    13081 aaggtactca tcgctttagc gacctgcggc gcaaaatggg cggggtgagt gaaaagatgc
    13141 ttgcgcagtc gttacaggca ttagaacagg atggttttct taaccgtatc gcgtatccgg
    13201 tggtgccgcc gcatgtggaa tatagcctca cgccgctggg cgagcaggtg agcgaaaagg
    13261 ttgctgcact ggcggactgg attgagttga atttgcccga ggtgttagcg gtgcgggatg
    13321 aacgtgcggc gtaactacag gatgcgcggc ctacggtgta tgacttgtag ggcggataag
    13381 atgcgccagc atcgcatccg ccaataaatg ccggatgcgg ttgatgattt gtaggccgaa
    13441 aaagaagtgt tttacttact cagatccacc tgataaatcg caaacccgat atcatcggtc
    13501 gcgactttat tcatcggata ctgccctttc tctttaataa acgctgcggc tttatctgac
    13561 ggagaggttt cgaaacggat atccagtttc ttatcgccag ctatcggtgc taaacgccag
    13621 ttgttatctg ccgccgggtg aatttccccc gcacgtttcg actcatcagc aatccacgct
    13681 gccagcaccg agcggttctc atccggtgaa gcaaaagcga tatggctgtc gcccgtaccg
    13741 gcaaatttgc cgccgtaagc gcgatagtta ttggtggcaa cgaggaacat ggcgttcgga
    13801 tcaatcggct tgccgttaaa ggtcaggttc ttaatccttt ccgcattggc attaatcatc
    13861 tggcactcgc cgtcataacg ggcgggctgg gtaacatcaa tctgataatt cacaccatca
    13921 ataacatcaa agttataggt gcggaaacca tcccagttga tgagtgactg tggtttcgtg
    13981 ctgttaggat caatctggtt aaactgtccg gcggagcact ccagccactc tttcacctct
    14041 ttaccgctgg ctttcaccac aatcagcgta ttggggtaga gataaagatc ggcggcatta
    14101 cggaaggtca actggccttt ttccacctcc acatagcttg ccgggtcatt tttgcgacca
    14161 ccaactttaa acggtgcggc agctgaaagc actggcagtt ttgccagatc cggatcgccc
    14221 tgaatgtaat gctcgacata cgctttttgc gcgttgttca ccacctgcac ggtcggatcg
    14281 tcctgcacaa gcgccagata gctatacata ttgtcggcag atttaccgat tggcttgctg
    14341 acgaactggc gtgtggcatc gtgatcggct ttgagtgttt ctaccagctt gctgtcttcc
    14401 gccgcgaggg attttttatt cgcgatgtcg taaatcggcc gtgcttccgc tttcgcctgc
    14461 gtcacctgcc atttaccgct gtcattactg agttgtaagt cgaccacacc aagatgatcg
    14521 ccccacatgc ctggcattac cgccggaaca ccattcagcg tgcctttcgc gatatcagcc
    14581 ccttcgatat cagcaaaatc tttacctggg aaaacggcgt gagcatggcc aaacataatg
    14641 gcgttaacgc ccggaatttc actgaggtaa taaactgagt tttccgccat cactttatac
    14701 ggatcggcag acagcccgga atgcgccaga acgacaacga catcggcacc tttctcgcgc
    14761 atttcaggca cgtatttgcg cacggtttcg gtaatatcat tcaccgtcac tttcccgaat
    14821 aaattagctt tatcccagcc catgatttgt ggcgggacga cgccaatata gccaatcttc
    14881 agcgtctgtt tttttccgtc tttatcgacc acttcggtgt ctttaattaa atacggtgta
    14941 aacattggct gtttggttct ggcgtcaatg acgttggcat ttacataagg gaatttcgct
    15001 cccgccagcg catttttcag ataatccaga ccgtagttaa actcgtggtt gccaagcgtg
    15061 ccgacggtat agtccagcgt atttaatgcc ttatagaccg ggtgaatatc acctgctttt
    15121 aatcctttcg ccgacatgta atctgccagc ggactcccct gaatcaaatc gccgttatcg
    15181 accagtacgc tgtttttcac ttcattgcgg gcatcgttaa tcaggcttgc cgtacgtacc
    15241 agtccgaatt tttccgtggc ggtgtctttg taataatcga aatccatcat gttgctatgc
    15301 agatcagtgg tttccatgat acgcagatcg accgtcgctg cattcacact ggcagcaatc
    15361 agcgtggcca ggagcgttgc gctaaactta atcatcaggg acatcctttt atcatcggga
    15421 atacgaaaga aaagggagaa taaacgtctt acttatagaa cagtgaagaa tgccacaatt
    15481 ttacgctttg aaaatgatga cactatcaca gttggcgcat tcattaacga tagggtataa
    15541 gtaaaacaat aagttaacac cgctcacaga gacgaggtgg agaaatgtta gatcaagtat
    15601 gccagcttgc acggaatgca ggcgatgcca ttatgcaggt ctacgacggg acgaaaccga
    15661 tggacgtcgt cagcaaagcg gacaattctc cggtaacggc agcggatatt gccgctcaca
    15721 ccgttatcat ggacggttta cgtacgctga caccggatat tccggtcctt tctgaagaag
    15781 atcctcccgg ttgggaagtc cgtcagcact ggcagcgtta ctggctggta gacccgctgg
    15841 atggtactaa agagtttatt aaacgtaatg gcgaattcac cgttaacatt gcgctcatcg
    15901 accacggcaa accgatttta ggtgtggtgt atgcgccggt aatgaacgta atgtacagcg
    15961 cggcagaagg caaagcctgg aaagaagagt gcggtgtgcg caagcagatt caggtccgcg
    16021 atgcgcgccc gccgctggtg gtgatcagcc gttcccatgc agatgcggag ctgaaagagt
    16081 atctgcaaca gcttggcgaa catcagacca cgtccatcgg ctcttcgctg aaattctgcc
    16141 tggtggcgga aggacaggcg cagctgtacc cgcgtttcgg gccaacgaat atttgggata
    16201 ccgccgctgg tcatgctgtg gctgcagctg ccggagcgca cgttcacgac tggcagggta
    16261 aaccgctgga ttacactccg cgcgagtcgt tcctgaatcc ggggttcaga gtgtctattt
    16321 attaattaat ttatgcagca ggtccatcac ttgctgcacc tcttcctgag taagcgcccc
    16381 gtctttggcc cattgcacgc gaccgtcttt atccagcacc actacagcgg aactttcttc
    16441 atccagctgc caggcaccgc gtgcgacgcc attgctatca acaataaact gcgaccaggg
    16501 ataaagcttt ttattactct ccagactgct gcgcacaaac atccctgaac ccggaatcgc
    16561 gtcgtcggtg ttaacaatgg tggtggtctg gtaacgatcg tgcggtaact tcgctgattt
    16621 aatcgcttca atcagcgtcg cgtttttctc ttttgcagag gtgcgaccag caatatgttg
    16681 cagtactcgc acttttccca ctaactgcgc gctgttccag gttttgtagc taaactgatc
    16741 tttatcaagc accaactcgc cacgatcggt gatgccaatc ggcggcactc gctgaccggt
    16801 ttcgaactga tgtgcggaag ccatcatcgg caacagcagg caggtgagtg ccagaatctt
    16861 gcgtagggtc atggtgtttc cttcttatga tatgcaggtg atccgaccac ttgggtctga
    16921 gttttaatca tatgtgctat ttatcgaatt tcccgcaagt gtgatgccag tttgcggtca
    16981 agcgcacaaa tcatatgaaa aatgaatgct tatactgaag accgcgcttc ggtaaaaaga
    17041 taattctgaa taattgtaac ctttaggtaa aaaaagttat acgcggtgga aacattgccc
    17101 ggatagtcta tagtcactag gcattaaaat ttgcgcctca taatagttgg gctgattgtg
    17161 gcaccgcaaa ggcgtaatac tcagcaggag ataacaatga aaattttcca acggtacaac
    17221 ccacttcagg tggcgaagta cgtaaagatc ctgttccgtg gacggttata catcaaggac
    17281 gttggcgctt ttgaatttga taagggtaag attcttatcc caaaagtgaa ggataagctg
    17341 catttgtcag tgatgtccga agttaaccgt caggttatgc gtctgcaaac agagatggct
    17401 taaccaaagt gctatgcagt aaaaattgct atgcagtaat aagacggctc ctgattcagg
    17461 agccgttgat gtttctgggg ttatgcgacg ctttcttctt tatctttagc gtcaggcagt
    17521 tttggcgaaa gggcggtggc tttgctgtcg attcgggtca ccagcagctg gtcgatgcgg
    17581 tagttatcga tatccaccac ttcaaatttg tagccggcga atttcaccga atcggtgcgt
    17641 ttcgggatct tacgcagcat aaacatcata aagccgccga tggtttcgta gttgcccgac
    17701 tgcgggaact cgtcaatatc cagcacgcgc atgacgtcgt caataggcgt gccgccgtct
    17761 atcagccatg agttctcatc acgggcgaca atctgctctt ccagcccctg accgaccaga
    17821 tcgcccatca gcgtggtcat cacgtcgttg agggtgatga tccccactac cagtgcgtac
    17881 tcgttcatga tcaccgcgaa gtcttcacct gcggttttaa aactttccaa cgcctctgaa
    17941 agggttaacg tatccggcac aatcagcgta ttgcgaattt gtacgccgct gttcagtgcc
    18001 aggctttggt tagccagcac gcggttcagc aggtctttag aatcgacata gccgatgatg
    18061 tggtcaatat cttcattaca gacgagaaac ttagagtgcg gatgttccgc caccttattc
    18121 ttcagacttt gctcatcttc gtggagatca aaccaaatca cgttttcacg cggtgtcatt
    18181 gaagacggaa cggtacggga ttccagctca aagacgtttt caatcagctc gtgttcctgt
    18241 ttacgtaaca cgcccgccag cgcaccggct tccactaccg cgtagatatc atcagaagtg
    18301 atgtcatctt tacgtaccat tggcagtttg aaaatacgga agattatgtt cgccagtccg
    18361 ttgaagaacc acaccagcgg ggtgcaaacg tacaggcaga agcgcatcgg gttgatgata
    18421 cgcaaagcca cagcttctgg cgcaatcata ccgatgcgtt tcggggttaa atccgcaaac
    18481 aggataaaca tgccagtcac taacgagaaa gagagaataa agctcagttg ctcagagagt
    18541 tctgccgaca tatagcggga gaacaggctg tgaaaagctg gagaaaatgc cgcatcaccg
    18601 acgataccgc cgagaatcgc cactgcgttc agaccgatct ggaccacggt aaagaacatg
    18661 ccgggatttt cctgcatatt caaaacgcgt tgggcattta tattgccttc atcagccagc
    18721 agtttaagtt tgattttgcg tgaggcggca agcgagatct cggacatcga gaagaacgca
    18781 cttacagcga tcaaacagag tatgactaaa atactgttta acatatctta tccgaccttt
    18841 caggccagat cctcggaagg ggaagttgat taatttgtgt gaaatacaca ttgaaagccg
    18901 attcgaagaa agtgaaccgg cattttcagt ggctagtata gcgtaaggta ctgtaaagcc
    18961 gccagagggt taaaattcag acagctgaaa aatgaaaaac tgcctgatgc gctatgccga
    19021 acaggcctac gcagtccccg caatatattg aatttggacg attttgtagg cctggcaagg
    19081 cgtttacgcc gtatccggca taaacaaaat gcactttatc aacaatctac atttgtaccc
    19141 gtaacgctat gcttccggcg gcagacagac gccaattccg ccaattccac agtaaccata
    19201 cgggttttta tgcagatatt gctggtggtc atcttcggca taataaaacg gtgtggcgtt
    19261 agcgatttcc gtggtgatgc gacgatcgtc atcggcggca agcatcgccg cctgaaaacg
    19321 ttccagactg gcgcgagcgg cggcatcctg ttctggggtc agcggataaa tcgctgaacg
    19381 atactgcgtg ccgtggtcat tgccctgacg cattccctgg gcgggatcgt gattctccca
    19441 aaatacttgt agcaactgct catagctgat gacggatgga tcgtaaacaa tgcgcaccgc
    19501 ttcggcatgg ccagtatcgc cggagcacac ttcccgataa gtcggatttg gcgtatagcc
    19561 gccggtatag cctgcggcgg tgctgtaaac gccgggtaac tgccagaaca gacgctccac
    19621 accccaaaaa caacccatcg caaaaatggc aatctccatt ccgtcaggta cattggtcat
    19681 tgagtgaccg ttgaccgcat gcagtgtggc tacgggcatc ggggtgttac gtccaggcag
    19741 ggcatcggcg ggggaaacca gatgcttttt atcaaataaa ctcatggtgt cgctctcccg
    19801 aaaatcggtc attggggtta aggttgtaac aagaggcgta tttgcacaca ataaccaccg
    19861 tgaataggtc taaagtaaaa cataagaaat attttgggtt tagtctgctt tttaatccat
    19921 attactggat ttttgttaag ccgtttaacg gcgttccagg ggcaggaaaa aaggatattc
    19981 aggagaaaat gtgcgctata tccgacagtt atgctgtgta agcttactct gcttaagcgg
    20041 atctgccgtc gccgcgaacg tccgtctaca ggtcgagggg ttatcgggag agctggaaaa
    20101 gaacgttcgc gcgcagcttt ctacgattga aagtgatgaa gtgacgccag accgtcgctt
    20161 tcgcgcacgc gtcgatgatg ccatccgcga aggtctgaaa gcgctgggct attaccagcc
    20221 gaccattgaa tttgatctcc gtccaccgcc aaagaaaggg cggcaggtat tgatcgccaa
    20281 agtcacgcca ggcgtgccgg tgttaattgg cggcaccgat gtggtattgc gcggcggcgc
    20341 gcggaccgat aaagactatt tgaaattgct cgatactcgc ccggctattg gtacggtact
    20401 gaaccagggc gattatgaaa atttcaaaaa gtccttaacc agcattgcgt tgcgtaaagg
    20461 ttatttcgat agcgaattta ccaaagcgca gctgggcatt gcgctcggcc tgcataaagc
    20521 cttctgggat attgattata acagtggcga acgttaccgc tttgggcatg tgacctttga
    20581 aggatcacaa atccgcgatg aatacctgca aaatctggtg ccgtttaaag agggcgatga
    20641 gtacgaatcg aaagatctgg cagaactgaa ccgtcgactt tctgctaccg gctggtttaa
    20701 ctcggtggtg gtggctccac aatttgataa agcgcgcgaa acgaaagtat taccattgac
    20761 gggcgtggtt tcgccgcgaa cagaaaacac catcgaaacc ggcgtcggtt actctacgga
    20821 cgtgggaccg cgcgtgaaag cgacgtggaa aaagccgtgg atgaactctt acggtcacag
    20881 tttgaccacc agtaccagta tttccgcgcc ggaacagatc ctcgacttca gctataaaat
    20941 gccgctgctg aagaatccgc tggaacaata ttatttggtg cagggcggtt ttaagcgcac
    21001 tgacctgaac gataccgagt ctgactccac tacgctggtg gcttctcgct actgggatct
    21061 ctccagcggc tggcagcgtg ccattaacct gcgctggagt ttcgaccact ttacccaggg
    21121 tgaaattacc aacaccacga tgctgtttta tcctggggtg atgattagcc gcacgcgttc
    21181 tcgtggtggc ctgatgccaa cctggggcga ctcgcaacgc tactctatcg actactccaa
    21241 cacggcctgg ggctcggatg ttgatttctc cgttttccag gcgcagaacg tctggatccg
    21301 cacactgtac gatcgccatc gttttgtgac acgcggcacg ctgggctgga ttgaaacggg
    21361 tgatttcgac aaagtaccgc cggatctgcg tttcttcgcc ggtggcgatc gcagtattcg
    21421 cggctacaaa tacaaatcta tcgccccgaa atacgccaac ggtgacctga aaggggcctc
    21481 gaagctgata accggatcgc tggaatacca gtacaacgtg accggaaaat ggtggggcgc
    21541 ggtgtttgtc gatagcggcg aagcggtaag cgatattcgc cgcagcgact ttaaaaccgg
    21601 taccggtgtc ggcgtgcgct gggaatcgcc ggtcgggcca atcaaactcg attttgccgt
    21661 gccggtcgcg gataaagacg aacacgggtt acagttttac atcggtctgg ggccagaatt
    21721 atgagtttat ggaaaaaaat cagcctcggc gtggttatcg ttatcttact gttgctggga
    21781 tcggtggcgt ttctggtggg caccaccagc ggcctgcatc tggtatttaa agcggcggat
    21841 cgctgggtgc caggactgga tattggcaag gtcaccggcg gctggcgtga tctcaccttg
    21901 tctgacgttc gttatgagca gccaggcgtg gcggtaaaag cgggcaatct gcatctggcg
    21961 gtcgggcttg agagcctgtg gaacagtagc gtttgtatta atgatctggc gctgaaagac
    22021 attcaggtca acatcgacag taaaaaaatg cctccttctg aacaggttga agaagaggaa
    22081 gatagcggtc cgctggatct ctccacgccg tatcccatta ccctgacacg ggtggcgctg
    22141 gacaacgtca acgtcaagat tgatgacacc acggtatcgg tgatggactt cacctccggc
    22201 ctgaactggc aggagaaaac cctgaccctg aaaccgacat cgctgaaagg cctgttgatt
    22261 gctttgccga aagtggcgga agcggcacag gaagaagtgg tcgaaccgaa aattgaaaat
    22321 ccgcagccgg aggaaaagcc gctcggcgaa acgctgaaag atctcttttc tcgcccggta
    22381 ttgccggaaa tgaccgacgt gcatttgccg cttaacctga acattgaaga gtttaagggc
    22441 gagcagctgc gcgtgacggg cgacacggac atcaccgtga gcaccatgct gctgaaagtg
    22501 agcagcattg acggcaatac taaactggac gccctggata tcgactccag tcaagggatc
    22561 gtcaacgcca gcggcacggc gcagctgtca gacaactggc cggtggatat caccctcaac
    22621 agcacactga acgtggagcc gttgaaaggt gaaaaggtga agctgaaagt gggcggtgcg
    22681 ctgcgcgaac agctggagat aggcgttaac ctttccggtc cggtggatat ggatttacgc
    22741 gcccagacgc gactggcaga agccggattg ccgctcaacg tggaagtgaa cagcaaacag
    22801 ctttactggc cgttcactgg tgagaagcag tatcaggcgg atgatctgaa actgaaactt
    22861 accggcaaaa tgaccgatta cgcgatctct atgcgcacgg ctgttaaggg ccaggagatc
    22921 ccgccagcca ccattaccct tgatgccaaa ggtaatgaac agcaggtcaa tctcgacaaa
    22981 cttaccgtcg cggcgctgga agggaaaacg gaacttaagg cgttgctcga ctggcagcag
    23041 gccattagtt ggcgcggtga gctaacgctt aacggcatta acaccgccaa agagatcccg
    23101 gagtggccgt cgaaactcaa tggcttgatt aaaacccgcg gtagcctgta cggcggcacc
    23161 tggcagatgg aggtgccaga actgaagctg accggtaacg tcaaacagaa caaagtgaac
    23221 gttgacggca cgctgaaagg caacagttat atgcagtgga tgatcccagg gctgcatctg
    23281 gaactggggc caaacagtgc cgaagtgaaa ggcgagctgg gggtaaaaga tctcaatctt
    23341 gatgccacca tcaacgcgcc ggggctggat aacgcgctgc cggggcttgg cggtaccgcg
    23401 aaagggctgg tgaaagtacg cggcacggtg gaagcgccac aactactggc agatatcacc
    23461 gcgcgcggcc tgcgctggca ggaactttcc gtggcgcagg ttcgcgtgga aggcgacatt
    23521 aaatccaccg atcagatcgc cgggaaactc gacgtacgcg ttgagcaaat ttcgcagccg
    23581 gatgtaaata tcaacctcgt caccctgaat gccaaaggca gtgaaaagca gcacgagcta
    23641 cagttgcgga ttcagggcga gcctgtctcc gggcagctta atctggcagg aagttttgat
    23701 cgcaaagaag aacgctggaa gggaactctt agcaataccc gcttccagac gccggtcggc
    23761 ccgtggtcgc tgacccgcga tattgcgctg gattaccgca ataaggagca aaaaatcagc
    23821 atcgggccac actgctggct taacccgaat gcggaactgt gcgtgccgca aactatcgat
    23881 gcgggtgccg aagggcgtgc ggtggtgaat ctcaaccgct tcgacctggc catgctgaaa
    23941 ccatttatgc cagaaaccac tcaggccagc ggtatcttca cgggtaaagc ggacgttgcc
    24001 tgggacacca cgaaagaggg gctgccgcag ggcagtatca ccctttcggg gcgtaatgtg
    24061 caggtgacgc aaaccgtcaa cgatgcggcg ctgcccgtgg cgtttcagac gctgaatctg
    24121 acggcggagt tgcgtaacaa ccgtgccgaa ttgggctgga ccatccgcct gaccaataac
    24181 ggccagtttg atggtcaggt gcaggtgacc gatccgcaag gccgccgtaa tcttggtggc
    24241 aacgtcaata tccgtaactt caaccttgcg atgataaacc ccatctttac ccgtggggaa
    24301 aaagcagcgg ggatggtaag tgccaacttg cgtctgggtg gtgatgtgca aagcccgcag
    24361 ttgtttggtc agcttcaggt tacgggggtg gatatcgacg gcaactttat gccgtttgat
    24421 atgcaaccaa gccagcttgc ggtcaacttt aacggtatgc gctcgacgct tgcaggtacc
    24481 gtgcggaccc agcagggtga aatctacctg aacggtgatg ccgactggag ccagattgaa
    24541 aactggcggg cgcgggtaac agcgaagggc agtaaagtgc gtatcaccgt gccgccgatg
    24601 gtacgaatgg atgtatcgcc agatgttgta ttcgaggcta caccaaacct gtttaccctc
    24661 gatggtcgcg tggatgttcc gtgggcgcgc atcgtggtgc acgatctgcc ggaaagcgca
    24721 gtaggcgttt ccagcgatgt ggtgatgctt aacgataacc tgcaaccgga agagccgaaa
    24781 acggcgtcga ttccgattaa cagtaacctg attgtccacg ttggcaacaa tgtgcgcatt
    24841 gacgcctttg gcctgaaagc gcggctgacg ggcgatctca acgtcgttca ggacaaacaa
    24901 gggctgggcc tgaacgggca gatcaacatc cctgaagggc gcttccatgc ctatggtcag
    24961 gatctgattg tgcgtaaggg tgagctactg ttctctggtc cgccagatca accgtatctt
    25021 aatatcgaag ctattcgtaa cccggatgct acagaagacg acgtcatcgc cggagttcgc
    25081 gtcactggtc tggcggacga accgaaagcg gagatcttct ctgacccggc gatgtcgcaa
    25141 caagctgcat tgtcttattt gctacgtgga caagggctgg agagcgatca gagcgacagt
    25201 gcggcaatga cctcgatgct gattggcctg ggggttgcgc aaagtggcca gattgtgggt
    25261 aaaatcggcg agacgtttgg cgtaagcaat ttagcgctcg acaccgaggg agttggcgac
    25321 tcctcccagg tggtggtcag cggctatgta ttgccaggcc tgcaagtgaa atacggcgtg
    25381 ggtatatttg actctatagc aacactcacg ttacgttatc gcctgatgcc taagctatat
    25441 ctggaagccg tgtctggtgt agaccaggca ctggatttgc tctatcagtt cgagttttag
    25501 caatgcgaat atttgtctac ggcagtttac gccacaaaca aggcaacagt cactggatga
    25561 ccaatgccca gttactgggc gatttcagta ttgataacta ccagttgtat agcctgggcc
    25621 actatccagg cgcagttccg gggaacggaa cagtacatgg tgaagtttat cgtattgaca
    25681 acgccacgct ggccgaactt gatgccttgc gcaccagggg cggtgaatac gcgcgccagt
    25741 tgattcagac gccgtacggg agtgcatgga tgtacgttta tcaacgaccc gtcgatggat
    25801 taaagctaat tgaaagcggc gactggttag acagggataa gtaataataa aagcacgcca
    25861 ccttcgggtg gcgttgtttt atccaccagc ctgaatatat tccgaatttg acgccattct
    25921 tatcttttct taaatgctga tttgttgcgc agtgttataa acacctggca cgctcaacaa
    25981 ggacgcgacc acaatgaaaa aatctcttct ggctgtaatg ctgacaggac tgtttgctct
    26041 cgtttctctt cccgctctgg gaaatgtcaa tctcgaacaa ttaaagcaaa aagctgaaag
    26101 tggagaagct aaagcacagt tggagctggg atatcgctat tttcagggta atgaaacgac
    26161 aaaggatctc acccaggcga tggactggtt tcgccgtgcc gcagagcagg gatacacccc
    26221 ggcagaatat gtactggggt tacgctatat gaacggtgaa ggtgtgccgc aagattatgc
    26281 tcaggcagtt atctggtaca aaaaagcggc actgaagggg cttccgcaag cgcagcagaa
    26341 tctgggtgta atgtaccatg aaggtaatgg cgtgaaggtt gataaagccg aatctgtgaa
    26401 atggtttcgc ctggcagtag agcaaggtcg tgacagcggc cagcaaagta tgggagacgc
    26461 atattttgaa ggcgatggtg tgacgcggga ttacgttatg gcacgtgagt ggtatagcaa
    26521 agcagcggaa caaggtaacg tctggtcctg taaccagctt ggttatatgt attccagagg
    26581 tttaggcgtt gaaagaaacg atgccatatc ggcacaatgg tatcgaaaat cagcgacatc
    26641 aggcgacgag ttggggcagc ttcatttagc cgatatgtac tatttcggta tcggtgttac
    26701 tcaggattac actcagtcac gggtattatt ttcccagtcg gcagagcagg gaaattctat
    26761 tgcgcagttt cgtctgggat atatattgga gcaaggttta gcgggagcga aagagccgtt
    26821 aaaagcactg gagtggtacc gtaaatcggc agaacaggga aattccgatg gtcagtatta
    26881 tttggcacat ctgtatgata aaggcgcaga aggtgtagca aaaaatcgag aacaagccat
    26941 ctcctggtac acgaaatcgg cagaacaggg ggatgctacc gcacaggcta atctcggcgc
    27001 tatttacttc agacttggtt cagaagaaga acataaaaag gcggtggaat ggtttcgcaa
    27061 ggccgccgca aagggtgaga aagcggcgca atttaatttg ggtaatgctt tactccaggg
    27121 caaaggtgtt aaaaaagatg agcaacaggc cgcaatctgg atgcgaaaag ccgcagagca
    27181 aggattaagt gcagcgcagg tacaattagg tgaaatctat tattatggct tgggcgtaga
    27241 acgtgattat gtgcaggcct gggcgtggtt cgataccgca tcgaccaatg atatgaatct
    27301 ttttggtaca gaaaaccgca acattacaga gaaaaaactg acagccaaac aactgcaaca
    27361 ggctgaatta ttatcgcaac aatatataga aaaatatgcc ccggaagcct gggcgagaat
    27421 gcaaaagctt aaagcgcaat cagcggtaaa gacgggtaat aaataacggg taactgtcag
    27481 atcaggtctg cgctaataca gctcaaccaa tgccacctgc gggtggcgtt atttttttgc
    27541 gagacgacgc gtattctttt ttgtaacttc ctcgcctttt gcttttcctt ccgagccgct
    27601 ttccatatcc gttaacgcat ataaaacttt gctggcgttc acaattactc aggggtaaaa
    27661 cgggtgcacg ctttgaacgg tgagtatgag tgaggccggc taacttccag caagtggcta
    27721 tgaacagcaa aatggtggtc gagtttagct tgttcttacc gttcaagcct ttaaactgct
    27781 gaaaataata atagtggagt agagagttta taaattgcgg gcataaaaaa acccctacta
    27841 accgaagccc ggcgttcagg gttattacgc tagaagaact tatttattct ttgcgcgctc
    27901 gaaggaggca acgatttcag ctttagcggc ttctgcgttt tcccaacctt caactttaac
    27961 ccacttgcct ttttcgaggt ctttgtagtg ctcgaagaag tgagcgattt gcgctttcag
    28021 cagctctggc agatcgttaa cgtctttaat gtgatcgtat tctttgctca gcttggtgtg
    28081 cggaacagca atcagtttcg catcttcacc ggcttcgtcg gtcattttca gtacgccaac
    28141 cggacggcag cggatcacag aacccggctg cagcgggtac ggagtcggga ccagtacgtc
    28201 aaccgggtca ccgtccagag acagggtgtg gttgatgtaa ccgtagttgc acggatagaa
    28261 catcgcggtg gacatgaagc ggtcaacgaa cagtgcgccg ctctctttgt cgatttcgta
    28321 tttgatcgga tctgcgttag ccgggatctc aataacaacg tagatgtctt ccggcagatc
    28381 tttacccgca gggacgttga gtaagctcat gtctgtttcc tttaaaaata tgtggcaaac
    28441 aagtgccgag tattatagcc aactcgcgcc gaatgtcttc gcttgttttc gtcttcattt
    28501 tcctttttcg ccagttttaa gacggtatac agagcaggaa aatccataac cgtagccgca
    28561 tttttcatag tgagatgaaa gcgattacaa acttgtgatt aacgttttat ttactttttt
    28621 gaagtgtgat gtaacgcaat ccgttacata acgaattgtc tatagttttt tcgcgaacat
    28681 cttttcacca ataataatta ccccgacgag gacaacccta tgtggaaacg cttacttgta
    28741 gtctctgcag tctcggcagc catgtcgtct atggcgttgg ccgctccatt aaccgtagga
    28801 ttttcgcagg tcggatcgga atccggctgg cgcgccgcag aaaccaatgt ggcgaaaagt
    28861 gaagccgaaa agcgcggaat tacgttgaaa attgccgatg gtcagcaaaa gcaggaaaac
    28921 cagattaaag cggtacgttc cttcgtcgcg caaggggtgg atgcgatctt tatcgctcca
    28981 gtggtagcga ccggttggga gccggtatta aaagaggcga aagatgccga aatcctggtc
    29041 ttcttgcttg accgttccat cgatgtgaaa gacaaatctc tctatatgac cactgtcacc
    29101 gccgacaaca tcctcgaagg caagttgatc ggtgactggc tggtaaaaga agtgaatggc
    29161 aaaccatgca acgtggtgga gctgcagggc accgttgggg ccagcgtcgc cattgaccgt
    29221 aagaaaggct ttgccgaagc cataaagaat gcgccaaata tcaaaattat ccgctcgcag
    29281 tcaggtgact tcacccgtag taaaggcaaa gaggtcatgg agagctttat caaagcggaa
    29341 aacaacggca aaaacatctg catggtttac gcccataacg acgacatggt gattggtgca
    29401 attcaggcaa ttaaagaagc gggcctgaag ccgggcaaag atatcctgac aggctctatc
    29461 gacggcgtgc cggacatcta caaagcgatg attgatggcg aagcgaacgc cagcgttgaa
    29521 ctgacgccga acatggcagg ccccgctttt gatgcgctgg agaaatataa aaaagacggc
    29581 accatgcctg aaaagctaac cctgaccaaa tccacccttt atctgcctga taccgcaaaa
    29641 gaagagttag agaagaagaa aaatatgggg tattgagggt tgctatgcct gatgccgatt
    29701 cgtaggccgg ataaggcgct cgcgccgcat ccggcggtgg tgcgctgaag cctgatgcga
    29761 cgcttaccgc gtcttatcat gcctactggg agcacgcttt acaccggggg aaaccatgac
    29821 gaccgaccaa caccaggaaa tcctccgcac cgaaggatta agtaaatttt tccccggtgt
    29881 caaagcgtta gacaacgttg atttcagcct gcgccgtggc gaaatcatgg cgctgctcgg
    29941 tgaaaacggg gcgggaaaat cgacgctaat caaagcatta accggtgtat accacgccga
    30001 tcgcggcact atctggctgg aaggccaggc tatctcaccg aaaaacaccg cccacgcaca
    30061 acaactcggc atcggcaccg tctatcagga agtcaacctg ctacccaata tgtcggtcgc
    30121 tgataatcta ttcataggtc gcgaacccaa acgtttcggc cttctacgcc gtaaagagat
    30181 ggaaaagcgc gccaccgaac tgatggcatc ttacggtttc tccctcgacg tgcgcgaacc
    30241 gctcaaccgc ttttcagtcg cgatgcagca aatcgtcgcc atttgccggg ctatcgatct
    30301 ctccgccaaa gtgctgatcc tcgatgaacc caccgccagt ctcgacactc aggaagtaga
    30361 gttactgttt ggcctgatgc gtcagcttcg cgatcgcggc gtcagcctga tcttcgtcac
    30421 tcattttctc gatcaggttt atcaggttag cgatcggatc accgtcttac gtaacggcag
    30481 ttttgtgggc tgtcgggaaa cccgcgagct accgcagatc gaactggtaa aaatgatgct
    30541 ggggcgtgaa ctggacaccc acgcgcttca gcgtgccggg agaacattgc tgagcgacaa
    30601 acctgttgcc gcgttcaaaa attacggcaa aaaaggaacg atcgcgccgt ttgatctcga
    30661 agtacgcccc ggcgagatcg tcggtctggc aggcttgctg ggatcaggac gtaccgaaac
    30721 cgccgaagtg atcttcggta tcaaacctgc tgatagcggc acggcgttga tcaaaggcaa
    30781 actgcaaacg ctacgctcgc cacatcaggc ttcggtactg ggcatcggct tctgcccgga
    30841 agacaggaaa accgatggca ttatcgccgc cgcctcggtg cgggaaaata tcatcctcgc
    30901 tctccaggcc cagcgcggct ggctgcgacc aatttcccgc aaagagcagc aagagattgc
    30961 cgaacgcttt attcgccagc ttggcattcg cacaccttcc accgaacaac cgattgaatt
    31021 tctctccggc ggcaatcagc aaaaagtgtt gctttcacgt tggctactga cccgaccgca
    31081 atttctgatt ctcgacgagc caacgcgcgg aattgatgtt ggtgcccacg ccgagatcat
    31141 ccgcctgatt gaaacgctgt gcgccgatgg gctggcgctg ctggtgatct cctccgagct
    31201 ggaagagctg gtgggctatg ccgaccgggt gattatcatg cgcgatcgca aacaggtagc
    31261 ggagatccca ctggcagagc tttccgttcc ggcgatcatg aacgccattg cggcgtaagg
    31321 agaacagtgt gatgcctcaa tctctcccgg acactacgcc gccgaaaagg cgttttcgct
    31381 ggccaacagg aatgccgcag ctggcggcac tgttgctggt gctgctggtt gatagcctgg
    31441 tggccccgca tttctggcag gtggtgcttc aggatgggcg tttgttcggt agccctatcg
    31501 acatttttaa ccgtgcggcc cccgttgcgc tactggcgat tggcatgacg ctggttatcg
    31561 ccaccggtgg gattgatctc tccgtagggg cggtgatggc tatcgccgga gccacaacgg
    31621 ctgcgatgac ggtcgcggga ttcagcctgc caattgtttt gttaagcgcc ctgggcactg
    31681 gcatcctggc gggattgtgg aacggcatac tggtagcgat cctcaaaatt cagccgtttg
    31741 tcgccaccct gatcctgatg gtcgccgggc gcggcgtggc gcaactgatc acctccggac
    31801 agatcgtcac atttaactca ccggatctct cctggttcgg cagtggatcg ctgttgttcc
    31861 tgccaacgcc agtcattatt gcggtgctga cgcttctcct gttctggctg ttgacccgca
    31921 aaacggcact ggggatgttt atcgaagccg ttggtatcaa cattcgggcg gcaaaaaatg
    31981 ccggggtaaa cacgcggatc atcgtcatgc tcacctacgt gttgagcggg ctatgtgcgg
    32041 cgattgcggg cattatcgtg gcggcggata ttcgcggtgc cgatgccaac aacgccggct
    32101 tatggctgga gctggacgcc attcttgcag tggtgattgg cggcggatcg ctgatgggcg
    32161 ggcgctttaa cctgctgctt tcggtggtgg gggcgctgat tattcaggga atgaacaccg
    32221 gaattttgct ttctggcttt ccgccggaga tgaaccaggt ggtgaaagcg gtggtggtgc
    32281 tttgcgtgct gattgttcag tcgcaacgct ttatcagtct gattaaaggg ggacgtaacc
    32341 gtgataaaac gtaatttgcc actgatgatc accatcggcg tctttgtgtt gggttatatc
    32401 tactgtctga cccagtttcc cggttttgct tccacaagag tgatctgcaa tatcctgacc
    32461 gataatgcct ttcttgggat aatcgccgtt ggcatgacct ttgtgatcct ctccggtggg
    32521 atcgatctct ccgtcggttc ggtgatcgcc tttaccggcg tgtttctggc aaaagtgatt
    32581 ggcgatttcg gcctctcgcc gctgctggcg tttccgctgg tgttggtgat gggctgtgcc
    32641 ttcggcgcat ttatggggct tctgattgac gccctgaaga tcccggcgtt tatcattacc
    32701 cttgcgggaa tgttcttttt gcgtggcgtc agctatctcg tttcggaaga gtcgatcccg
    32761 ataaaccatc ctgtttatga cacgctctca agccttgcgt ggaaaatccc tggcggtggt
    32821 cgcttaagtg cgatgggact actgatgctg gcggtggtgg ttatcggcat attactcgcg
    32881 catcgtaccc gttttggtaa tcaggtatac gccattggcg gcaacgcaac gtcggcaaac
    32941 ctgatgggga tttccactcg cagcaccact attcgcattt atatgctctc caccggactg
    33001 gcaacgctgg cggggattgt cttctcgatt tatacccagg ccggatatgc actggcgggc
    33061 gtaggtgtgg aactggatgc tatcgcctca gtggtaattg gcggtacgct tttgagtggt
    33121 ggcgttggaa cggtattagg gacgcttttt ggcgtggcga ttcagggact gattcagact
    33181 tacataaact ttgatggcac gctgagttcc tggtggacga aaatcgccat cggtattttg
    33241 ttgtttattt ttatagcatt acagcgtgga ttaacggtgc tgtgggagaa tcgtcagagt
    33301 tcgccagtga caagagtcaa cattgcgcag cgataaaaac gcctctccgt gtggagaggc
    33361 gcaggagctt acgcgtccgg gaactcacgg ataaagcgtt cgacatcttc aaccatatgg
    33421 tcgttgccga caaagaacga acggcgctgg tgcagggttt ccgggataat atccagaata
    33481 cgctctttgc catcgctcgc tttaccgccc gcttgttccg ccaggaatgc catcgggttg
    33541 cactcataca gtaaacgtag tttaccgtcc gggtggctgg cggtgcttgg gtagagataa
    33601 ataccgcctt tcagcaggtt acggtggaaa tccgcgacca gtgaaccgat ataacgtgag
    33661 gtataagggc ggttggtgga cttatcttct tcctggcaga atttaatgta cttcttcacc
    33721 ccgttcggaa acttaatgta gtttccttcg ttgatggagt aggttttgcc tttctccggg
    33781 aagcgcatcc gttcctggca caggcagaaa acacccagcg aaggatcata agtaaaggcg
    33841 tgaacaccgc atccggtggt gtaaaccagc atagtagagg agccgtatac cacgtaacct
    33901 gccgcaacct gtttgttacc aggctggagg aaatcttctt ccgttaccgg cgtgccaaca
    33961 ggcgtaacgc ggcggtagat agagaaaatg gtaccgacag agacgttaac atcgatgttg
    34021 gacgagccat ccagtgggtc catcagcacc acgtattttg cgtgttcaca gccttcaaag
    34081 acgacaatct catcttcttc ttcagaggca atgcccgcaa cgatatcgcg tgctttcagt
    34141 gcggctttca gtttctcatt agcgaacagg tcgagtttct gctgaacctc gccctgcacg
    34201 ttctcagcac cgctggcacc caggatatca accagtcctg ctttgttgat atcgcgatgg
    34261 ataatcttgg cgcccagttt tattgccgac agcaaagcag tgagctcacc ggtagcatga
    34321 gaaaactcgt gctgcttttc gacaataaat tcacctaacg ttttcataaa actttccctg
    34381 caatgtttat ggagtaaagc gaccgcaaca atcttaacaa ataatcccaa tgttgcgctc
    34441 aggtgaatcg cgccagcaaa ttacggatta tcctgaaatg cgtttctcac ttgcccgaca
    34501 tatgcgtaaa atgagcgcca gattaaaaaa ggatagtgac gtatgcgcat tcatatttta
    34561 ggaatttgtg gcacgtttat gggcggtctg gcgatgctgg cgcgccagtt aggccatgaa
    34621 gtaacgggtt cggacgccaa tgtgtatccg ccgatgagca ccttacttga gaagcaaggc
    34681 attgaactga ttcagggtta cgatgccagc cagctcgatc cacagccgga tctggtgatt
    34741 attggtaacg ccatgacccg tggaaatccg tgtgtggaag cggtactgga aaaaaacatc
    34801 ccttatatgt caggtccgca gtggctgcac gattttgtgc tgcgcgaccg ctgggtgctg
    34861 gccgttgccg gtacacacgg caaaaccacc acggcgggaa tggcgacctg gattctggaa
    34921 caatgcggtt acaaaccggg atttgtgatc ggcggtgtgc cggggaactt tgaggtttcg
    34981 gcgcgtctgg gcgaaagcga cttctttgtt atcgaagcgg atgagtacga ctgcgccttc
    35041 ttcgacaaac gctctaaatt tgtccattac tgcccgcgta cgctgatcct caacaacctt
    35101 gagttcgatc acgccgatat ctttgacgac ctgaaagcga tccagaaaca gttccaccat
    35161 ctggtgcgta tcgttccggg gcaggggcgt attatctggc cggaaaacga tatcaacctg
    35221 aaacagacca tggcgatggg ctgttggagc gagcaggagc tggtgggcga gcaaggtcac
    35281 tggcaggcga aaaagctgac cactgacgct tctgaatggg aagtcttgct ggatggcgaa
    35341 aaagtgggcg aagtgaaatg gtcgctggta ggcgaacata atatgcacaa tggcctgatg
    35401 gcgattgcgg cggctcgcca tgttggtgta gcgccggcag atgccgctaa cgcgctgggt
    35461 tcgtttatta acgctcgtcg ccgtctggag ttgcgtggtg aagcgaatgg cgtcacggta
    35521 tatgacgatt ttgcccatca cccgacggcg attctggcaa cgcttgcggc gctgcgtggc
    35581 aaagttggtg gtacggcgcg cattattgcc gtgctggaac cgcgctcaaa caccatgaaa
    35641 atggggatct gcaaagacga tctggcacct tcattaggtc gtgccgatga agtcttcctg
    35701 ctgcaaccgg cgcatattcc gtggcaggtg gcagaagtgg cagaagcctg cgttcagcct
    35761 gcacactgga gcggcgatgt ggatacgctg gcagatatgg tggtgaaaac cgctcagccg
    35821 ggcgatcaca ttctggtgat gagtaacggc ggttttggtg ggatccatca gaaactgctg
    35881 gatgggttag cgaagaaagc ggaagccgcg cagtaattaa cagcctcagc ctgatagagt
    35941 gtgtgtaggc cggataaggc gttcacgccg catccggcat ttgagcatag tgcctgatgc
    36001 gacgcttgat gcgtcttatc aggcctacag gtgcaccgta tccggcaaac catactcaca
    36061 tcaacaacga aaattaccct tcgttctccg ctaactcgcg cagatactgg aaaatctggc
    36121 gtgcggattt cggcggctta ttcccttctt tctctttctt cgcgttgcgg atcagagttc
    36181 gcagttgctg acgatcggca tcgggccaca gattcaatac ttcagcgatg gcgtcatcac
    36241 cctggtcgat cagacgatcg cgcaggtttt ccagtttatg gaacagcacc acctgctggt
    36301 tgtgacggtt tttcagctta tccagcgcct gacggatcgg ctcaacatcg cgctggcgca
    36361 gcatcttacc gatgagctgc aactggcggc ggcgaccttc catcttaata cgctgggcga
    36421 gctcaatagc cgcgcgcaga tccgcatcta acgggatctt atccagcgcg tttttcccca
    36481 gatcaacaat ttctgcgccc aggcgtttta gctcctcggc atcgcgttta atttcacttt
    36541 tactgaccca gataatttca tcgtcttcgt cttcgatgtc atcaccggga acgtcgtcga
    36601 gccagtcttc gggctgctta gtcatctcag gctccttaaa aaaagaggct aatgttacca
    36661 gttaagatgc gcactgaaaa acggttctct gttagacttc agagaaactc tctacattat
    36721 ggcacttgca atgaaagtaa tctctcaagt tgaagcgcag cgcaagattc tggaagaagc
    36781 agtttcgact gcgctggagt tggcctcagg caaatcggac ggtgcagaag ttgccgtcag
    36841 caagaccacc ggcattagcg taagcacgcg ttatggtgaa gtggagaatg ttgaattcaa
    36901 tagcgatggc gcgctgggga tcaccgttta tcaccagaac cgtaaaggta gcgcatcgtc
    36961 taccgattta agcccgcagg ccattgcccg caccgtacag gcggcgctgg atattgcccg
    37021 ttacacttcg ccagatccgt atgcaggcgt ggcagacaaa gaactgctgg cctttgacgc
    37081 accggatctc gacttgttcc accctgcgga cgtttctccg gatgaagcca ttgaactggc
    37141 ggcccgtgct gaacaggcgg cattacaggc ggataaacgc attaccaaca ccgaaggcgg
    37201 tagctttaac agccactacg gtgtcaaagt ttttggcaac agccacggca tgttgcaggg
    37261 ttactgctca acgcgtcatt cgctctccag ttgtgtaatc gccgaagaaa atggcgatat
    37321 ggagcgtgat tacgcctaca ccattgggcg tgcgatgagc gatctgcaaa cgccagagtg
    37381 ggtcggggcc gactgtgctc gccgtacctt atcgcgcctg tcaccgcgta aactttcgac
    37441 catgaaagcg ccggtgattt ttgccaatga agtggcaaca gggttgtttg gtcatttggt
    37501 gggggcgata gcgggtggtg cggtctaccg taaatcgacc ttcttgctgg attcgctggg
    37561 taaacaaatt ctgccggact ggctgactat tgaagaacat ccgcatctgc tgaaagggct
    37621 ggcatctaca ccattcgaca gcgaaggtgt gcgcaccgag cgccgcgata tcgtcaaaga
    37681 tggcatcctg actcagtggc tgctgaccag ctattcggcg cgcaaattgg ggctgaaaag
    37741 caccggacat gcgggcggta ttcacaactg gcgcattgcc ggacaaggtc taagcttcga
    37801 gcagatgctc aaagagatgg gcactgggct ggtggtgacg gaattgatgg gccagggcgt
    37861 gagtgccatt accggtgatt attcccgtgg tgcagcgggc ttctgggtag agaatggcga
    37921 gattcagtat ccggtgagcg aaatcaccat cgctggtaat ttaaaagata tgtggcgcaa
    37981 tattgtcacc gtcggtaacg atattgaaac acgcagtaat atacagtgtg gttctgtgct
    38041 gttgccggag atgaaaatcg ccgggcagta aaaaactggc gcgacctgcc gcgccaaatc
    38101 ttcataataa aaaaggaagt gagcaatgcg taaaagcctg ttagctattc ttgcagtctc
    38161 ctcattggta ttcagttcgg cgtcgtttgc cgctgatctg gaagacaata tggaaaccct
    38221 caacgacaat ttaaaagtgg tcgaaaaagc cgataacgcg gtgcaagtca aagacgcgtt
    38281 aactaaaatg cgcgctgcgg cgctggatgc gcaaaaagcg acgccgccga agcttgaagg
    38341 caaatcaccg gacagcccgg aaatgaaaga cttccgccac ggtttcgaca ttctggtcgg
    38401 tcagattgac gacgcgctga agctggcaaa tgaaggtaaa gtaaaagaag cgcaggctgc
    38461 cgcagagcaa ctgaaaacga cccgcaacgc ctatcaccag aagtatcgtt aattctttat
    38521 ttccctgttg cctgcactca ggtaacaggg aacttatcat cgcaaatgat gcaccacctg
    38581 attgctgctg ccgcgccaga tcagggaagg gtcttttaaa tcctgcacaa atttgccgtc
    38641 gaccagcacg ttaatcaaat caacaacctg catttgcgca gcgttgagtt cgtcgagttt
    38701 atagcctgtc cacacccaga tgtctttacc cggacactcg gcgcggatgc gttgtaccag
    38761 tttcagaata tccggcacgt tttgcggatg tagcggatcg ccgccggaga gggaaatacc
    38821 ctggcgtttg atacgagtgt cattcagatc gttaatgatc tggtcttcca ttgctttggt
    38881 aaatggctga ccggaattta cccgccaggt gcttttgtta tagcaaccgg ggcattcatg
    38941 aacacacccg gagacaaaca gggtgcagcg agtgccgggg ccgttgacga tgtcgacagg
    39001 atagtactga tgatagttca ttatttcacc tggcttatca gtccgcgtgt aggctgcatc
    39061 cggttatcaa cgcctgatgc gacgctgtcg cgtcttatca ggcctacagc cgttgccaaa
    39121 tgtaggccgg ataaggcgtt tacgccgcat ccggcatttg cttaacacag agtgaagatt
    39181 aacctatctg cccatttccc aaatgtttaa cgcggcgctt aacttcttcc tgcttaccag
    39241 cgttaaacgg acgtgcatcc gggctaccta aatatccgca cacgcggcga gtcaccgaca
    39301 cacgggaggt gtcatggtta ccacatttcg gacaagtgaa gcctttgctg gtgcactcaa
    39361 attcgccggt aaagccacac tcgtagcatt catcgatcgg tgtgttggtg ccgtaatacg
    39421 gtacatgctg atagctgtaa tcccagacat cttccagcgc cttcaggttg tgctgaatgt
    39481 ttggatactc gccgtagcaa atgaaaccac cgttcgccag cggcgggtaa ggcgcttcaa
    39541 agtcgatctt gtcatacggg ttcacctttt tctccacatc aaggtggaaa ctgttggtgt
    39601 agtaaccttt atcggtcacg cccggcacca cgccaaactc agcggtatcg agacggcaga
    39661 agcggtcgca caggttttca cttggcgtgc tgtagagact gaaaccataa cccgtttctt
    39721 ctttccactg atccaccgcc tgacgcagac gctcaacaat cgcaatacct ttcgcgcgca
    39781 gctgctcgtt gtcgtaaaca tgctcgccgc cgaacagcgc gttaatggtt tcgtggatgc
    39841 cgatgtaacc cagcgaaata gacgcacgac cgtttttgaa gatttcagaa acgtcgtcgt
    39901 cagcattcag acgcacgcca caagcacctt ccatatagag gatcggggcc acgcgcgctt
    39961 tcacgccttc gagacgagcg atacgggtca tcaacgcctt acgtgccagc accagacgtt
    40021 catccagcag cttccagaag gtggcttcat cgccttttgc ctccagagca atacgcggca
    40081 ggttcaggct gatcacgccg aggttgttac gaccatcgtg gatctgctcg ccgttttcat
    40141 tttcccacac gccgaggaag ctgcggcagc ccatcggggt tttaaacgaa ccggtgactt
    40201 tcactacctg atcgtagttc aggatatccg gatacatgcg cttgcttgcg cactccagcg
    40261 ccagctgttt gatgtcgtag ttcggatcgc cttttttatg gttcaggcca tcgcgaatcg
    40321 caaacaccag tttcgggaac accgcagttt tacggttttt accgaggcct gcgatacggt
    40381 tacgcaggat agattcctga atcaggcgcg actcccagct ggtgcccaga ccaaaaccaa
    40441 aggtcacaaa cggtgtttga ccgttggcgg tgtgcagggt gtttacttcg tactccagcg
    40501 actggaaggc atcgtagcac tctttgatgg tacgagagtt agcgtagcct tcggcgtccg
    40561 ggatgttcca ctcttctgcg gtcttgcgat gtttgttgta gctggcggtg acaaacggtg
    40621 ccagcacttc atcgatacgg ttaatggtgg tgccgccata aatatggctg gcgacctgag
    40681 caataatctg cgcagttaca gcggttgccg tagagatcga cttcggcggt tcaatctcgg
    40741 cgttccccat tttaaagccc tgggtcagca tgcctttcag gtcgatcaac atgcagttga
    40801 acatcgggaa gaacggcgag taatcgagat cgtgatagtg aatatcacca cgttcatgtg
    40861 cctgcaccac gtcacgcggc agcaggtgct ggcgtgcata gtgtttagcc acgatcccgg
    40921 ccagcaggtc gcgctgggtt ggaatcacct tgctgtcttt gttggcgttt tcgttgagca
    40981 acgaggcgtt ggtctgctca accagaccac ggatctcctg attcaggcga ccgcgttttt
    41041 cacgttcaat gtcgcgatcg tgacggtact cgatgtaagc acgagccagt tgtttgtatg
    41101 gacccgacat aagctggttt tcaacggcgg tctggatctc gttgatatcc acctggctgc
    41161 ggccctgcat ctgctcgctg acaaccgcgg caacagtggc gcaataatct gcatcatcga
    41221 cttccgctgc tttagctgca cgcagaatcg cttctttgat gcgctctgat ttaaacggca
    41281 ctttgcagcc gtctcgtttc atcacatgcg gtgtcatgat cgctccatat ttttaagaac
    41341 aggttatcca cagaaattgg gaaaggcgtt ttccggtttt ttcgtttcgt tttccggtgc
    41401 tttccgcaat cgccatccgc ttatccacaa cgccgcacct ttttcgtggg gagctgtagt
    41461 agcaattata gtcgattaat acaacatatt gggttgggac gcattttaaa gtctatatat
    41521 agtgctttgc atcaaggatg tttgaacttt ttttgatgta gctcaaagta aaaagcagag
    41581 cgtacggatg acgggcgcta cagcgatatg taaatttttt aatgaatttt ttgattataa
    41641 aatcaacaaa aacaacatac tgacaggcaa aaccccggaa tgacaggcat taccggggct
    41701 tagcgaagat ttacttctgt aaccaccaga cagcctcaaa aggccgtaaa gtcatggtac
    41761 agggttgtgg tgaggcttct tcgtagttat gcatcacaag ctgccagttg ccgagcattt
    41821 tccctggctg ccagggttgg gtctcacggc taaggttggc aatgaccagc aaggtttgcc
    41881 ccttccactc acggcgatag caccacaata cagggctgtt tggcagcaga tcctggtaat
    41941 cgccccatgt caggacggct tcttgcttac gcagtgcgat taacttttgg taggtgtaaa
    42001 acaccgagga ctcatcggcc agcgctgctt ctacgttgat ttcctgatag ttatcgccca
    42061 ggccaatcca cggttcgcca gccgtaaacc cggcattatc gccgttggtc cattgcatgg
    42121 gtgtgcgact gttgtcacgg gatttactgg cgaggattgt caataactcg tcggcatcac
    42181 gcccatcgtt gcgcagctcg gcaaacatat tgaggctctc cacgtcgcga tagtcagtaa
    42241 tgcgcgtgaa atgtgggttg gtcatgccaa tctcttcgcc ctggtagata tacggcgttc
    42301 cctgcatgcc atgcagcacc atcgccagca tttttgccgc aggcacgcgg tattcacctt
    42361 catcaccaaa gcgagaaaca atgcgcggct gatcgtggtt acaccagaac aaggcattcc
    42421 atgccacgtt gtgcattcct tgttgccagt ggcggaacaa tgttttcaac gccacaaagt
    42481 caggtttagc cagcgtccat ttttcaccac cgtgataatc gaccttcagg tgatgaaaat
    42541 taaaggtcat cgacaattca ctgcctgtca gtgccgcgta tcgctggcaa tgctcaaggc
    42601 tggtggagga catttcacct acggtcatta acccgcgtgg cgtaaacaca tcgcggttca
    42661 tctcgtgcaa aaactcgtgt gctcgtggcc cgtcggtata gaagcgacgc ccatcgccat
    42721 ccaggtcttc agggtaacgc gggtctttgg agatcagatt caccacatcc aggcgcaacc
    42781 cgtcgacccc acgatcggcc cagaactcac agactttttt cagctctgcg cgtaccgctg
    42841 gattctccca gttgagatcc gcctgttctg gtgcaaagag atgcaaatag tactgttcgc
    42901 tttctgcatg ccagcgccac gcactaccac cgaattttga acgccagttg ttcggtggcg
    42961 tttctggttc tccatcgcgc cagatataaa actggcggta agggctttct ttgttcagcg
    43021 cctcgcgaaa ccaggcatgt tgggtagagg tatggttaaa caccatatcg agaatgatac
    43081 gaatcccacg cgattttgcc tgtgtcacca gttcgtcaaa atcgtccagc gtgccatagg
    43141 tgggatcaat cgccgtataa ttcgctacgt cgtaaccgtt atcgacctgg ggagagacat
    43201 aaaagggggt tagccagatg gcatcaacgc ccagtttatg cagatagtcc aggcgttgga
    43261 taacgccacg taaatcgccg gtgccgctac ccgtggtgtc ctgaaaactc tttggataaa
    43321 tctggtagat aacgccgttt tgccaccagt ggggaagatg agtcattacg ttattcctgc
    43381 aaatgcgagg gggcgcaatt gcgccccgaa gaaaattaaa caatgtccag cgtgcccagg
    43441 cggtatttcc gctgatagat aaacgaggtg agtacaatcg ggatgatgat ggcgatagcc
    43501 attgccagcg caaacacctg ccagtagctc ggttgaatcg agagaattcc cggcaggccg
    43561 cctacgccga tgccattcgc cataacgccg ttcaggccgc atagcaatcc tgccagacca
    43621 gaaccaatca tcgcgcacag catcgggaag cgatatttca ggttgatgcc gtacatagca
    43681 ggctcagtga ctccaagcca ggcggagata gcggcaggca cggagatctc gcgttcattg
    43741 tgcttgcgac tggaaatgat aatacctatc acggcggagc cctgagcaat attcgacagc
    43801 gcaatcagcg gccacactgg cgtgccaccc atgctttgaa tcatctgcaa atcaatagca
    43861 agcgtggtct ggtgtacgcc ggtgatcacc agcggggcgt acaggaagcc aaacaatgcc
    43921 gcgccaatcg gagcaaagct gccggtcatc aggtgacgta ccgcaaaggc aacgccatcg
    43981 ccaatcatgc gaccaaacgg accaatcagc gcatgggcga ggaacaccgc gaggatcagc
    44041 gaacagacgg gcaccaccac cagatagagg taatccggca cgatgcgctt aagacgagtt
    44101 tcaataacgc ccagcgccag tccggctaac agtgccggga tgacctgcgc ctgatagccc
    44161 actttggcga tgctgaacat gccaaagtcc catacttccg gcagctgctg cccgagcaga
    44221 taagcgttca tcagctgtgg agaaaccagc gttacgccaa gcacgatacc gaggatcggt
    44281 gtaccgccca tttttttcac cgctgaccag caaatcccga ccggcaggta gaagaagatc
    44341 gcttcaccga tcaaccacag aaaatcgtag atcgtttgca gggaagggta catttgcgcc
    44401 agcgtctgac cgttgctcat gggcaaatcg ccgatcacat tgcgaaaacc gaggatcaaa
    44461 ccaccactaa tcaacgcggg cagcaacggg aagaagatct ccgcgaaatg agagatcaac
    44521 tgctcatgcc atttcatatt ctgccgggcg gcttttttta cctgctcttt atcaacctgc
    44581 gcctgtccgg ttgacgcaat cagtgcttga tagtaatcac ccacgttggt gccaatcacc
    44641 acctgaaatt gcccggcatt ggtgaaacag cctttcacca tggggagttg ctcaatttct
    44701 ttcggtctgg cattggccgg ttggttgagg acaaagcgca ggcgagtaat acagtggctc
    44761 accgtcgcaa tattgccgcg cccgccgacc agttcaatca accgatcgat atccgtttgg
    44821 tttattttgc tcatcataaa gccccatggc agatgacatt tttggttggc tgcagaatat
    44881 attgcgcgga aaaatttaaa aacgggaacg ttcccgaaac gcagcgaaga tcacaattta
    44941 tcgttcagga aacgatcagg acagggtggc ggggatgatg atttgttgcg gttcgctgcg
    45001 cccggttacc tgcgcgatca actggcaggc cgcctggcgt ccagcttcgg cataaccggg
    45061 atctacggtg acgatctccg gatggaggaa tttcattaac ggtgtattac cgacgctcgc
    45121 cagttgcaag gtgtcgatgc gttgctcttg caggtattta cttgcgccaa gtgccagcgt
    45181 gtcggttgcg cacagtaacg cggtagtttc aggcgtaatc acttttgcta cgttctcata
    45241 gccttgcttc atagcaagcc ctggcagagc ggcaacggga tgcaatttat gcgttttgca
    45301 gaacgcaagg taggcttcgt gacgtcgctt accggttgtc acgtcactgt gcggcacgcc
    45361 gagatagctg atattacgat gtccctggtc atacagccgt tgcatcagga tcttgattgc
    45421 cccttcgtcg tcataacaga ccgaagcaaa gccttttgcg tcacgcgcca gcagaaccag
    45481 cgatgactgc cagtgggcta acatttcttc tgttatgccg gtaaaaccga acagcactac
    45541 gccgtcgata ttacgccgtt tcagcacccc caaatgttcg gcaactaatt gtggggaaaa
    45601 ctgactttcc atcatgattg gatcgtaacc ttgttcatag aacgctggca gcatggtttg
    45661 aacggcgaga ttttctgaca acgaatccag acgggtaaca atgatggcga ccactttatc
    45721 gctttgccca cgcatagcgc gcgcagagcg ggaaggggaa aatccatgct gattcatcac
    45781 tgcttcaaca cgctcgcgcg tgcgctggct cacgccgctt tcgttattca gcacccggga
    45841 aaccgtagat ttccccacgc cgcttaagcg cgcgatgtct ttgatggtca gccgattttg
    45901 catcctgttg tcccgtaacg tgttgtttaa ttatttgagc ctaactttac ctgtgcattc
    45961 agcaatgggt aaagtctggt ttatcgttgg tttagttgcc agcaggtatt atatcgccat
    46021 aaatgctacg aatattattg gattctcctt attatttgcg gcgctttttt cacttaccgg
    46081 aggttatatg gaacctgatc ccacgcctct ccctcgacgg agattaaaac ttttccggta
    46141 agcccgtctt ttcacggcgt taccggatgc gtaaggccgt gacgttttaa cgtccctgct
    46201 cagctttatt accttcaggt aaggcttcgc cacgcctgaa gacatttctg tactgtttca
    46261 gacagtgcgg agggactcct tatgtttaaa gaaattttta cccggctcat tcgccattta
    46321 ccttcccgtc tggttcatcg tgatccattg cctggcgcgc agcagacagt gaatgcggcg
    46381 gtcccgcctt ctttaagtgc gcattgcctg aaaatggcgg tgatgcccga agaagaattg
    46441 tggaaaacgt tcgacaccca tccggaaggg ttaaatctgg cggaagtgga atctgcccgc
    46501 gaacaacatg gtgaaaataa attacccgca caacaaccgt cgccgtggtg ggtacattta
    46561 tgggtctgct atcgcaaccc ctttaatatt ttgcttacca ttctcggtgc tatttcttac
    46621 gccacggaag atttatttgc cgcaggtgtt atcgcgctaa tggtcgctat ttctacgttg
    46681 ctgaacttta ttcaggaagc tcgttccacc aaagcggcag atgccctgaa agcgatggtc
    46741 agtaataccg cgacggtact gcgtgtaatt aacgataaag gtgaaaatgg ctggctggag
    46801 atcccgatag accagctggt gcccggcgat attataaaac tggcggcagg ggatatgatc
    46861 ccggcagatt tacgtatctt gcaggcgcgg gatctgttcg tcgctcaggc gtcgttaacc
    46921 ggtgagtctc tgcccgtgga aaaagccgct actactcgcc agccggagca cagcaatccg
    46981 ctggagtgcg acaccctgtg ttttatgggc accaccgtgg tgagcggcac ggcacaagca
    47041 atggtgattg ctacaggagc caacacctgg tttggtcaac tggcggggcg tgttagtgag
    47101 caggaaagcg agccgaatgc ctttcagcaa gggatcagcc gcgtcagtat gctgctgatt
    47161 cgctttatgc tggtgatggc tccggtggtg ttgcttatta atggttacac taaaggcgac
    47221 tggtgggaag ctgcactgtt tgcgctttcg gtcgcggtag gcttaacgcc agaaatgttg
    47281 ccgatgattg ttacctcgac gctggcgcgt ggcgcagtca agttgtcgaa acagaaagtg
    47341 atcgtcaaac atctggatgc cattcagaac tttggcgcaa tggatattct gtgcactgat
    47401 aaaaccggca ccctgacaca ggataaaatt gtgctggaga atcataccga tatctccggt
    47461 aaaactagcg aacgcgtgct gcatagcgcg tggttgaaca gccattacca gaccgggctt
    47521 aaaaacctgc ttgatacagc ggtgctcgaa ggtacggatg aagagtcagc gcgctcgctg
    47581 gccagccgtt ggcaaaaaat tgatgagatt ccgtttgatt tcgagcgtcg tcggatgtcg
    47641 gtggttgtgg cagaaaatac cgaacatcat cagttggttt gcaaaggcgc attgcaggaa
    47701 atcctcaatg tgtgttcgca agtgcgtcac aatggcgaga ttgtaccgct cgatgacacc
    47761 atgctgcgta agattaagcg ggttactgat acgctgaatc gtcaggggct gcgcgtggtt
    47821 gcggtggcga cgaaatacct gccagcgcgt gaaggagatt accagcgggc ggatgaatcc
    47881 gatctgatcc tcgaaggata tattgctttc cttgatccgc caaaagagac aacagctccg
    47941 gcactgaagg cattaaaagc gagtggaatt accgtaaaaa tccttactgg cgacagtgag
    48001 ttagttgcag cgaaagtgtg ccatgaagtg gggctggacg cgggagaggt ggtcattggt
    48061 agtgatattg aaacgctatc tgacgacgaa ctggcaaatc tcgcacagcg caccacgctg
    48121 tttgctcgtc tgacgccgat gcataaagaa cgcatcgtaa cgttgctgaa gcgcgaaggg
    48181 catgtggttg gctttatggg cgatggtatt aatgatgcgc ccgccttacg cgctgcggat
    48241 atcggcattt ctgtggacgg cgcggtagat attgcccgtg aagcggctga tatcatccta
    48301 ctggaaaaaa gcctgatggt gctggaagag ggggttattg agggacgtcg cactttcgcc
    48361 aacatgctga aatacatcaa aatgacggcg agctctaact tcggtaatgt gttcagcgtg
    48421 ctggtggcga gtgctttctt gcccttcctg ccgatgttgc cgctacactt gcttattcaa
    48481 aatctgctgt acgatgtgtc acaggtggcg atcccatttg ataacgtcga cgacgagcaa
    48541 attcaaaagc cgcagcgttg gaatccggcg gatctggggc gctttatggt cttcttcgga
    48601 ccgatcagct cgattttcga tattctgacg ttttgcctga tgtggtgggt attccatgcc
    48661 aacacgccgg aaacgcaaac gctgttccag tcgggctggt ttgtggtggg cttactgtcg
    48721 caaacgctga ttgtgcatat gatccgcacc cgtcgtgtac cgtttattca gagctgtgca
    48781 tcgtggccgt taatgatcat gaccgtgatc gtgatgattg tcgggatcgc attgcctttt
    48841 tcaccgctgg ccagttatct gcaactgcag gcgctgccgt taagctattt cccgtggctg
    48901 gttgcgattc tggcagggta tatgacgtta acccagttgg tgaaagggtt ctatagccgt
    48961 cgttacggtt ggcagtaaag aataaactgg gcatggtggc ccggaatcag attgcagaca
    49021 aagtgagctt tgttcatgcc ggatgcggcg taaatgcctt atcaggccta caaattcaat
    49081 aaattgcagg aatcacgtag gcctgataag cgtagcgcat caggcaattt tgcgtttgtc
    49141 attagactta atccgggcat gatagcccgg atttccatca agattagcga cgaacagcga
    49201 tcgcttcgat ctcaatcttc acgtctttcg gcagacgggc aacttcaacg caagaacgtg
    49261 ccgggaaggt ggcgttgtgt tcggtgaaga aggcttcgta ggtagcatta acggttgcga
    49321 agtcgttcag atctttcaca aacacggtag ttttaacgat gtcgcccact ttcaggccag
    49381 cggcttcgac gatcgctttt acgttatcca gcgactgacg cgcctgtgca gcgacatctg
    49441 ccgggacttc gcccgttttc gggtttaccg ggatctgacc ggaagtgata atcatattgc
    49501 ccagatcaac gccctgtacg taaggaccga tagctgccgg tgcattttcc gtcgcgatag
    49561 ttttgctcat gatttctcct ttattacagc ggtaaaaagt ctggctcatt atagggagcc
    49621 agacttttat taccaaccgc aattaattgg ccagcaccac attatgagaa aactcttttt
    49681 cacagtattt gcatttgagc gcgatatcat tggcgcgttt tcgcacggca aagctggatg
    49741 aaaccggttc ggcatggctg atacagttgc tgttcgggca gaccagcaca ttgtcgatgc
    49801 gctctggcag actggggcgc gatttgccaa ccacttcata gttgtcgata cggttaaccg
    49861 tggcttgcgg tgcatataac gccagctgat caacctgatc ctcacttaaa aaggtatttt
    49921 cgattttgat cagatccttg cgccccatct cgccagaagg caggttcaga ccaatggtga
    49981 tgcgctgatc tgtttcggtc aacttgaaca gactcaacag tttaaaaccg atctgggcgg
    50041 ggatatggtc aattaccgtg ccgcgtttaa tagcttcaac ctgcaattta ttatcgtgtg
    50101 tcatctctat ttccccttac agtaccagat cgcgattcag aaccagtgcc agtaacgcct
    50161 ggcgagcgaa aatcccgttg cctgcttgct ggaagtacca ggcgtgaggc gttttatcca
    50221 catccgtcgc aatctcatca acacgcggca gcggatgcag cactttcata ttggctttgg
    50281 cgttgtggag atcgctggcg cgaagaacaa actgcgcttt cacgttggcg tactcggacg
    50341 ggtccagacg ctctttttgc acgcgggtca tgtacaggat gtctacttcc gccatcactt
    50401 cttcaataga gctgtgcaga ctccaggcga tccctttttc atcgagcata tccagaatgt
    50461 attgcggcat tgccagcgca tccggcgcga taaagtaaaa acggttgccg tcgaacttcg
    50521 ctagcgcctg ggtcagggag tgaacggtgc ggccatattt caggtcacca accattgcga
    50581 cgtggagatt gtccagacgc ccctgggttt cctgaatggt gaataagtcc agcaaggttt
    50641 gcgtcggatg ttggttggag ccatcaccgg cattcagtac cggtacattg ccggaaaact
    50701 cggtggccag gcgcgctgca ccttcctgcg gatgacgcat cactatcgca tcgacgtaag
    50761 tgctgataac cgaaatggta tcggccagcg tttcgccctt tttacccagt gatgtattgg
    50821 cgctgtcgga gaagcccacc acgctggcac ccaggcggtg catggaagtt tcgaaagaga
    50881 ggcgggtgcg ggtagaggct tcgaagaaac agctggcaat aactttgtgc ttcaacagct
    50941 ctggttgcgg gttcgctttc agtttcgccg ctgtcgccag caccagatta aggtcatcgc
    51001 gactaaggtc gtttatggaa atgatatgtt tctgatatag cggattagcc atcttttatc
    51061 tcctgacgcc tgggcaaaaa aaagcccctc gattgagggg ctgggaatgg gtgatcaacg
    51121 ggaagaaaaa cggcagacca gcgtcttttt tcagacgcgg taagacaaaa tgtcgaacac
    51181 actgaaccat acatcctccc ggcaaattgt ccggcattat actcagctct gagagaggat
    51241 caagaagatg atgcagttat aaagcaaggc aaacggttat catgaatata tattcaattt
    51301 taataa
//
LOCUS       DADSYM010000010        47924 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2415
            SAMN08039579-rid11723173.denovo.010, whole genome shotgun sequence.
ACCESSION   DADSYM010000010 DADSYM010000000
VERSION     DADSYM010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039579
            Sequence Read Archive: SRR6362533
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 47924)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 47924)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980162.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 03:39:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,484
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 4,347
            CDSs (with protein)               :: 4,347
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 43 of 85
            Pseudo Genes (incomplete)         :: 37 of 85
            Pseudo Genes (internal stop)      :: 19 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..47924
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039579-rid11723173.denovo.010"
                     /strain="LGE2415"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8302 N 95.9628 W"
                     /collection_date="2015-08-24"
     gene            439..1656
                     /locus_tag="J1Y83_000489"
     CDS             439..1656
                     /locus_tag="J1Y83_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HBA7628675.1"
                     /translation="MSPIEKSSKLENVCYDIRGPVLKEAKRLEEEGNKVLKLNIGNPA
                     PFGFDAPDEILVDVIRNLPTAQGYCDSKGLYSARKAIMQHYQARGMRDVTVEDIYIGN
                     GVSELIVQAMQALLNSGDEMLVPAPDYPLWTAAVSLSSGKAVHYLCDESSDWFPDLDD
                     IRAKITPRTRGIVIINPNNPTGAVYSKELLMEIVEIARQHNLIIFADEIYDKILYDDA
                     EHHSIAPLAPDLLTITFNGLSKTYRVAGFRQGWMVLNGPKKHAKGYIEGLEMLASMRL
                     CANVPAQHAIQTALGGYQSISEFITPGGRLYEQRNRAWELINDIPGVSCVKPRGALYM
                     FPKIDAKRFNIHDDQKMVLDFLLQEKVLLVQGTAFNWPWPDHFRIVTLPRVDDIELSL
                     SKFARFLSGYHQL"
     gene            1740..2339
                     /gene="yfbR"
                     /locus_tag="J1Y83_000490"
     CDS             1740..2339
                     /gene="yfbR"
                     /locus_tag="J1Y83_000490"
                     /EC_number="3.1.3.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-deoxynucleotidase"
                     /protein_id="HBA7628676.1"
                     /translation="MKQSHFFAHLSRLKLINRWPLMRNVRTENVSEHSLQVAMVAHAL
                     AAIKNRKFGGNVNAERIALLAMYHDASEVLTGDLPTPVKYFNSQIAQEYKAIEKIAQQ
                     KLVDMVPEELQDIFAPLIDEHAYSDEEKSLVKQADALCAYLKCLEELAAGNNEFLLAK
                     TRLEATLEARRSQEMDYFMEVFVPSFHLSLDEISQDSPL"
     gene            complement(2398..4230)
                     /locus_tag="J1Y83_000491"
     CDS             complement(2398..4230)
                     /locus_tag="J1Y83_000491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HBA7628677.1"
                     /translation="MNGELIWVLSLLAVAIVLFATGRVRMDAVALFVIVAFALSGTLT
                     VPEVFSGFSDPNVVLIAALFIIGDGLVRTGVATVMGTWLVKVAGNSEIKMLVLLMLTV
                     AGLGAFMSSTGVVAIFIPVVLSVAMRMQTSPSRLMMPLSFAGLISGMMTLVATPPNLV
                     VNSELLREGYHGFSFFSVTPIGLVVLVLGILYMLVMRFMLKGDTQTPQREGWTRRTFR
                     DLIREYRLTGRARRLAIRPGSPMIGQRLDDLKLRERYGANVIGVERWRRFRRVIVNVN
                     GVSEFRARDVLLIDMSAADVDLRQFCSEQLLEPMVLRGEYFSDQALDVGMAEISLIPE
                     SELIGKSVREIGFRTRYGLNVVGLKRNGVALEGSLADEPLLLGDIILVVGNWKLIGML
                     AKQGRDFVALNLPEEVSEASPAHSQAPHAIFCLVLMVALMLTDEIPNPVAAIIACLLM
                     GKFRCIDAESAYKSIHWPSIILIVGMMPFAVALQKTGGVALAVKGLMDIGGGYGPHMM
                     LGCLFVLSAVIGLFISNTATAVLMAPIALAAAKTMGVSPYPFAMVVAMAASAAFMTPI
                     SSPVNTLVLGPGNYSFSDFVKLGVPFTIIVMAVCVVMIPMLFPF"
     gene            complement(4317..4967)
                     /gene="hxpA"
                     /locus_tag="J1Y83_000492"
     CDS             complement(4317..4967)
                     /gene="hxpA"
                     /locus_tag="J1Y83_000492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexitol phosphatase HpxA"
                     /protein_id="HBA7628678.1"
                     /translation="MRCKGFLFDLDGTLVDSLPAVERAWSNWARRHGLSPEEVLAFIH
                     GKQAITSLRHFMAGKSEADIAAEFTRLEHIEATETEGITALPGAIALLNHLNKAGIPW
                     AIVTSGSMPVARARHKIAGLPAPEVFVTAERVKRGKPEPDAYLLGAQLLGLAPQECVV
                     VEDAPAGVLSGLAAGCHVIAVNAPADTPRLNEVDLVLHSLEQITVTKQPNGDVIIQ"
     gene            complement(4978..5472)
                     /locus_tag="J1Y83_000493"
     CDS             complement(4978..5472)
                     /locus_tag="J1Y83_000493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007371080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbU family protein"
                     /protein_id="HBA7628679.1"
                     /translation="MEMTNAQRLILSNQYKMMTMLDPANAERYRRLQTIIERGYGLQM
                     RELDREFGELKEETCRTIIDIMEMYHALHVSWSNLQDQQSIDERRVTFLGFDAATEAR
                     YLGYVRFMVNVEGRYTHFDAGTHGFNAQTPMWEKYQRMLNVWHACPRQYHLSANEINQ
                     IINA"
     gene            complement(5555..6010)
                     /locus_tag="J1Y83_000494"
     CDS             complement(5555..6010)
                     /locus_tag="J1Y83_000494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF412 domain-containing protein"
                     /protein_id="HBA7628680.1"
                     /translation="MSTPDNRSVNFFSLFRRGQHYSKTWPLEKRLAPVFVENRVIKMT
                     RYAIRFMPPIAVFTLCWQIALGGQLGPAVATALFALSLPMQGLWWLGKRSVTPLPPAI
                     LNWFYEVRGKLQESGQVLAPVEGKPDYQALADTLKRAFKQLDKTFLDDL"
     gene            6348..7550
                     /gene="ackA"
                     /locus_tag="J1Y83_000495"
     CDS             6348..7550
                     /gene="ackA"
                     /locus_tag="J1Y83_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HBA7628681.1"
                     /translation="MSSKLVLVLNCGSSSLKFAIIDAVNGEEYLSGLAECFHLPEARI
                     KWKMDGNKQEAALGAGAAHSEALNFIVNTILAQKPELSAQLTAIGHRIVHGGEKYTSS
                     VVIDESVIQGIKDAASFAPLHNPAHLIGIEEALKSFPQLKDKNVAVFDTAFHQTMPEE
                     SYLYALPYNLYKEHGIRRYGAHGTSHFYVTQEAAKMLNKPVEELNIITCHLGNGGSVS
                     AIRNGKCVDTSMGLTPLEGLVMGTRSGDIDPAIIFHLHDTLGMSVDAINKLLTKESGL
                     LGLTEVTSDCRYVEDNYATKEDAKRAMDVYCHRLAKYIGAYTALMDGRLDAVVFTGGI
                     GENAAMVRELSLGKLGVLGFEVDHERNLAARFGKSGFINKEGTRPAVVIPTNEELVIA
                     QDASRLTA"
     gene            7625..9769
                     /gene="pta"
                     /locus_tag="J1Y83_000496"
     CDS             7625..9769
                     /gene="pta"
                     /locus_tag="J1Y83_000496"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HBA7628682.1"
                     /translation="MSRIIMLIPTGTSVGLTSVSLGVIRAMERKGVRLSVFKPIAQPR
                     TGGDAPDQTTTIVRANSSTTTAAEPLKMSYVEGLLSSNQKDVLMEEIIANYHANTKDA
                     EVVLVEGLVPTRKHQFAQSLNYEIAKTLNAEIVFVMSQGTDTPEQLKERIELTRNSFG
                     GAKNTNITGVIVNKLNAPVDEQGRTRPDLSEIFDDSTKAKVNNVDPAKLQESSPLPVL
                     GAVPWSFDLIATRAIDMARHLNATIINEGDINTRRVKSVTFCARSIPHMLEHFRAGSL
                     LVTSADRPDVLVAACLAAMNGVEIGALLLTGGYEMDARISKLCERAFATGLPVFMVNT
                     NTWQTSLSLQSFNLEVPVDDHERIEKVQEYVANYINADWIDSLTATSERSRRLSPPAF
                     RYQLTELARKAGKRIVLPEGDEPRTVKAAAICAERGIATCVLLGNPAEINRVAASQGV
                     ELGAGIEIVDPEVVRENYVGRLVELRKNKGMTETVAREQLEDNVVLGTLMLEQDEVDG
                     LVSGAVHTTANTIRPPLQLIKTAPGSSLVSSVFFMLLPEQVYVYGDCAINPDPTAEQL
                     AEIAIQSADSAAAFGIEPRVAMLSYSTGTSGAGSDVEKVREATRLAQEKRPDLMIDGP
                     LQYDAAVMADVAKSKAPNSPVAGRATVFIFPDLNTGNTTYKAVQRSADLISIGPMLQG
                     MRKPVNDLSRGALVDDIVYTIALTAIQSAQQQ"
     gene            9959..11479
                     /gene="yfcC"
                     /locus_tag="J1Y83_000497"
     CDS             9959..11479
                     /gene="yfcC"
                     /locus_tag="J1Y83_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HBA7628683.1"
                     /translation="MSAITESKPTRRWAMPDTLVIIFFVAILTSLATWVVPVGMFDSQ
                     EVQYQVDGQTKTRKVVDPHSFRILTNEAGEPEYHRVQLFTTGDERPGLMNFPFEGLTS
                     GSKYGTAVGIIMFMLVIGGAFGIVMRTGTIDNGILALIRHTRGNEILFIPALFILFSL
                     GGAIFGMGEEAVAFAIIIAPLMVRLGYDSITTVLVTYIATQIGFASSWMNPFCVVVAQ
                     GIAGVPVLSGSGLRIVVWVIATLIGLIFTMVYASRVKKNPLLSRVHESDRFFREKQAD
                     VEQRPFTFGDWLVLIVLTAVMVWVIWGVIVNAWFIPEIASQFFTMGLVIGIIGVVFRL
                     NGMTVNTMASSFTEGARMMIAPALLVGFAKGILLLVGNGEAGDASVLNTILNSIANAI
                     SGLDNAVAAWFMLLFQAVFNFFVTSGSGQAALTMPLLAPLGDLVGVNRQVTVLAFQFG
                     DGFSHIIYPTSASLMATLGVCRVDFRNWLKVGATLLGLLFIMSSVVVIGAQLMGYH"
     gene            complement(11512..12054)
                     /gene="yfcD"
                     /locus_tag="J1Y83_000498"
     CDS             complement(11512..12054)
                     /gene="yfcD"
                     /locus_tag="J1Y83_000498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase YfcD"
                     /protein_id="HBA7628684.1"
                     /translation="MEQRRLASTEWVDIVNEENEVIAQASREQMRAQCLRHRATYIVV
                     HDGMGKILVQRRTETKDFLPGMLDATAGGVVQADEQLLESARREAEEELGIAGVPFAE
                     HGQFYFEDKNCRVWGALFSCVSHGPFALQEDEVSEVCWLTPEEITARCDEFTPDSLKA
                     LALWMKRNAKNEAVETETAE"
     gene            complement(12112..12663)
                     /gene="yfcE"
                     /locus_tag="J1Y83_000499"
     CDS             complement(12112..12663)
                     /gene="yfcE"
                     /locus_tag="J1Y83_000499"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311211.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiesterase"
                     /protein_id="HBA7628685.1"
                     /translation="MKLMFASDIHGSLPATERVLELFAQSGAQWLVILGDVLNHGPRN
                     ALPEGYAPAKVAERLNEVAHKVIAVRGNCDSEVDQMLLHFPITAPWQQVLLEKQRLFL
                     THGHLFGPENLPALNQNDVLVYGHTHLPVAEQRGEIFHFNPGSVSIPKGGHPASYGML
                     DNDVLSVIALNDQSIIAQVAINP"
     gene            complement(12719..13363)
                     /gene="yfcF"
                     /locus_tag="J1Y83_000500"
     CDS             complement(12719..13363)
                     /gene="yfcF"
                     /locus_tag="J1Y83_000500"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione transferase"
                     /protein_id="HBA7628686.1"
                     /translation="MSKPAITLWSDAHFFSPYVLSAWVALQEKGLSFHIKTIDLDSGE
                     HLQPTWQGYGQTRRVPLLQIDDFELSESSAIAEYLEDRFAPPTWERIYPLDLENRARA
                     RQIQAWLRSDLMPIREERSTDVVFAGAKKAPLTAEGKASAEKLFAMAEHLLALGQPNL
                     FGEWCIADTDLALMINRLVLHGDEVPERLVDYATFQWQRASVQRFIALSAKQSG"
     gene            13499..14146
                     /gene="yfcG"
                     /locus_tag="J1Y83_000501"
     CDS             13499..14146
                     /gene="yfcG"
                     /locus_tag="J1Y83_000501"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GSH-dependent disulfide bond oxidoreductase"
                     /protein_id="HBA7628687.1"
                     /translation="MIDLYFAPTPNGHKITLFLEEAGLDYRLIKVDLGKGGQFRPEFL
                     HISPNNKIPAIVDHSPADGGEPLSLFESGAILLYLAEKTGLFLSHETRERAATLQWLF
                     WQVGGLGPMLGQNHHFNHAASQTIPYAIERYQVETQRLYYVLNKRLENSPWLGGENYS
                     IADIACWPWVNAWTRQRIDLAMYPAVKNWHERIRSRPATGQALLKEQHGDERSDS"
     gene            14203..14565
                     /gene="folX"
                     /locus_tag="J1Y83_000502"
     CDS             14203..14565
                     /gene="folX"
                     /locus_tag="J1Y83_000502"
                     /EC_number="5.1.99.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate 2'-epimerase"
                     /protein_id="HBA7628688.1"
                     /translation="MAQPAAIIRIKNLRLRTFIGIKEEEINNRQDIVINVAIHYPADK
                     ARTSEDINDALNYRTVTKNIIQHVENNRFSLLEKLTQDVLDIAREHHWVTYAEVEIDK
                     LHALRYADSVSMTLSWQR"
     gene            14586..15479
                     /locus_tag="J1Y83_000503"
     CDS             14586..15479
                     /locus_tag="J1Y83_000503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01777 family protein"
                     /protein_id="HBA7628689.1"
                     /translation="MNIVITGGTGLIGRHLIPRLLDLGHQITVVTRNPQKASSVLGPR
                     VTLWQGLAEQSNLNGVDAVINLAGEPIADKRWTHEQKERLCQSRWNITQKLVDLINAS
                     DTPPSVLISGSATGYYGDLGEVVVTEEEPPHNEFTHKLCARWEEIACRAQSDKTRVCL
                     LRTGVVLAPDGGILGKMLPPFRLGLGGPIGSGRQYLAWIHIDDMVNGILWLLDKELRG
                     PFNMVSPYPVRNEQFAHALGHALHRPAILRVPATAIRLLMGESSVLVLGGQRALPKKL
                     EEAGFAFRWYDLEEALADVVR"
     gene            complement(15527..16417)
                     /locus_tag="J1Y83_000504"
     CDS             complement(15527..16417)
                     /locus_tag="J1Y83_000504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HBA7628690.1"
                     /translation="MTISTTSTPHDAVFKSFLRHPDTARDFIDIHLPAPLRKLCDLTT
                     LKLEPNSFIDEDLRQYYSDLLWSVKTQEGAGYIYVVIEHQSKPEELMAFRMMRYSIAA
                     MQNHLDAGYKELPLVIPMLFYHGCRSPYPYSLCWLDEFAEPAIARKIYSSAFPLVDIT
                     VVPDDEIMQHRKMALLELIQKHIRQRDLLGLVDQIVSLLVTGNTNDRQLKALFNYVLQ
                     TGDARRFRAFIGEIAERAPQEKEKLMTIADRLREEGAMQGKHEEALRIAQEMMEKGFD
                     HEVILTLTRLSPDDLIAQSH"
     gene            complement(16613..17386)
                     /gene="hisP"
                     /locus_tag="J1Y83_000505"
     CDS             complement(16613..17386)
                     /gene="hisP"
                     /locus_tag="J1Y83_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter ATP-binding protein
                     HisP"
                     /protein_id="HBA7628691.1"
                     /translation="MSENKLNVIDLHKRYGEHEVLKGVSLQANAGDVISIIGSSGSGK
                     STFLRCINFLEKPSEGSIVVNGQTINLVRDKDGQLKVADKNQLRLLRTRLTMVFQHFN
                     LWSHMTVLENVMEAPIQVLGLSKQEARERAVKYLAKVGIDERAQGKYPVHLSGGQQQR
                     VSIARALAMEPEVLLFDEPTSALDPELVGEVLRIMQQLAEEGKTMVVVTHEMGFARHV
                     STHVIFLHQGKIEEEGAPEQLFGNPQSPRLQQFLKGSLK"
     gene            complement(17394..18110)
                     /locus_tag="J1Y83_000506"
     CDS             complement(17394..18110)
                     /locus_tag="J1Y83_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBA7628692.1"
                     /translation="MIEILHEYWKPLLWTDGYRFTGVAITLWLLILSVVIGGVLALFL
                     AIGRVSSNKYIQFPIWLFTYIFRGTPLYVQLLVFYSGMYTLEIVKGTEFLNAFFRSGL
                     NCTVLALTLNTCAYTTEIFAGAIRSVPHGEIEAARAYGFSTFKMYRCIILPSALRIAL
                     PAYSNEVILMLHSTALAFTATVPDLLKIARDINAATYQPFTAFGIAAVLYLIISYVLI
                     SLFRRAEKRWLQHVKPSSTH"
     gene            complement(18107..18793)
                     /locus_tag="J1Y83_000507"
     CDS             complement(18107..18793)
                     /locus_tag="J1Y83_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311219.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HBA7628693.1"
                     /translation="MLYGFSGVILQGALVTLELAISSVVLAVIIGLIGAGGKLSQNRL
                     SGLIFEGYTTLIRGVPDLVLMLLIFYGLQIALNTVTEAMGVGQIDIDPMVAGIITLGF
                     IYGAYFTETFRGAFMAVPKGHIEAATAFGFTRGQVFRRIMFPAMMRYALPGIGNNWQV
                     ILKSTALVSLLGLEDVVKATQLAGKSTWEPFYFAIVCGVIYLVFTTVSNGVLLFLERR
                     YSVGVKRADL"
     gene            complement(18883..19665)
                     /gene="hisJ"
                     /locus_tag="J1Y83_000508"
     CDS             complement(18883..19665)
                     /gene="hisJ"
                     /locus_tag="J1Y83_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter substrate-binding
                     protein HisJ"
                     /protein_id="HBA7628694.1"
                     /translation="MKKLVLSLSLVLAFSSATAAFAAIPQNIRIGTDPTYAPFESKNS
                     QGELVGFDIDLAKELCKRINTQCTFVENPLDALIPSLKAKKIDAIMSSLSITEKRQQE
                     IAFTDKLYAADSRLVVAKNSDIQPTVESLKGKRVGVLQGTTQETFGNEHWAPKGIEIV
                     SYQGQDNIYSDLTAGRIDAAFQDEVAASEGFLKQPVGKDYKFGGPSVKDEKLFGVGTG
                     MGLRKEDNELREALNKAFAEMRADGTYEKLAKKYFDFDVYGG"
     gene            complement(19886..20668)
                     /gene="argT"
                     /locus_tag="J1Y83_000509"
     CDS             complement(19886..20668)
                     /gene="argT"
                     /locus_tag="J1Y83_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine/arginine/ornithine ABC transporter
                     substrate-binding protein ArgT"
                     /protein_id="HBA7628695.1"
                     /translation="MKKSILALSLLVGLSTAASSYAALPETVRIGTDTTYAPFSSKDA
                     KGDFVGFDIDLGNEMCKRMQVKCTWVASDFDALIPSLKAKKIDAIISSLSITDKRQQE
                     IAFSDKLYAADSRLIAAKGSPIQPTLDSLKGKHVGVLQGSTQEAYANETWRSKGVDVV
                     AYANQDLVYSDLAAGRLDAALQDEVAASEGFLKQPAGKDFAFAGPSVKDKKYFGDGTG
                     VGLRKDDAELTAAFNKALNELRQDGTYDKMAKKYFDFNVYGD"
     gene            complement(20934..21503)
                     /locus_tag="J1Y83_000510"
     CDS             complement(20934..21503)
                     /locus_tag="J1Y83_000510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HBA7628696.1"
                     /translation="MKRLIVGISGASGAIYGVRLLQVLRDVTDIETHLVMSQAARQTL
                     SLETDFSLREVQALADVTHDARDIAASISSGSFQTLGMVILPCSIKTLSGIVHSYTDG
                     LLTRAADVVLKERRPLVLCVRETPLHLGHLRLMTQAAEIGAVIMPPVPAFYHRPQSLD
                     DVINQTVNRVLDQFAVTLPEDLFARWQGA"
     gene            complement(21598..23115)
                     /gene="purF"
                     /locus_tag="J1Y83_000511"
     CDS             complement(21598..23115)
                     /gene="purF"
                     /locus_tag="J1Y83_000511"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006820991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HBA7628697.1"
                     /translation="MCGIVGIAGVMPVNQSIYDALTVLQHRGQDAAGIITIDANNCFR
                     LRKANGLVSDVFEARHMQRLQGNMGIGHVRYPTAGSSSASEAQPFYVNSPYGITLAHN
                     GNLTNAHELRKKLFEEKRRHINTTSDSEILLNIFASELDNFRHYPLEADNIFAAIAAT
                     NRLIRGAYACVAMIIGHGMVAFRDPNGIRPLVLGKRDIDDNRTEYMVASESVALDTLG
                     FEFLRDVAPGEAIYITEEGQLFTRQCADNPVSNPCLFEYVYFARPDSFIDKISVYSAR
                     VNMGTKLGEKIAREWEDLDIDVVIPIPETSCDIALEIARILGKPYRQGFVKNRYVGRT
                     FIMPGQQLRRKSVRRKLNANRAEFRDKNVLLVDDSIVRGTTSEQIIEMAREAGAKKVY
                     LASAAPEIRFPNVYGIDMPSATELIAHGREVDEIRQIIGADGLIFQDLNDLIEAVRAE
                     NPDIQQFECSVFNGVYVTKDVDQGYLDFLDTLRNDDAKAVQRQNEVENLEMHNEG"
     gene            complement(23152..23640)
                     /gene="cvpA"
                     /locus_tag="J1Y83_000512"
     CDS             complement(23152..23640)
                     /gene="cvpA"
                     /locus_tag="J1Y83_000512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin V production protein"
                     /protein_id="HBA7628698.1"
                     /translation="MVWIDYAIIAVIAFSSLVSLIRGFVREALSLVTWGCAFFVASHY
                     YTYLSVWFTGFEDELVRNGIAIAVLFIATLIVGAIVNFVIGQLVEKTGLSGTDRVLGV
                     CFGALRGVLIVAAILFFLDSFTGVSKSEDWSKSQLIPQFSFIIRWFFDYLQSSSSFLP
                     RA"
     gene            complement(23899..24561)
                     /gene="dedD"
                     /locus_tag="J1Y83_000513"
     CDS             complement(23899..24561)
                     /gene="dedD"
                     /locus_tag="J1Y83_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311225.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DedD"
                     /protein_id="HBA7628699.1"
                     /translation="MASKFQNRLVGTIVLVALGVIVLPGLLDGQKKHYQDEFAAIPLV
                     PKAGDRDEPDMMPAATQALPTQPPEGAAEEVRAGDAAAPSLDPATIAANNTEFEPEPA
                     PVVPPKPKPVEPPKPKVEAPPAPKPEPKPVVEEKAAPTGKAYVVQLGALKNADKVNEI
                     VGKLRGAGYRVYTSPSTPVQGKITRILVGPDASKDKLKGSLGELKQLSGLSGVVMGYT
                     PN"
     gene            complement(24551..25819)
                     /gene="folC"
                     /locus_tag="J1Y83_000514"
     CDS             complement(24551..25819)
                     /gene="folC"
                     /locus_tag="J1Y83_000514"
                     /EC_number="6.3.2.12"
                     /EC_number="6.3.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tetrahydrofolate
                     synthase/dihydrofolate synthase"
                     /protein_id="HBA7628700.1"
                     /translation="MIIKRTPQAASPLASWLSYLENLHSKTIDLGLERVSQVAARLGV
                     LKPAPFVFTVAGTNGKGTTCRTLESILMAAGYKVGVYSSPHLVRYTERVRVQGQELPE
                     SAHTASFAEIESARGDISLTYFEYGTLSALRLFKQAQLDVVILEVGLGGRLDATNIVD
                     ADVAVVTSIALDHTDWLGPDRESIGREKAGIFRSEKPAIVGEPEMPSTIADVAQEKGA
                     LLQRRGVEWNYSVTDHDWTFSDAHGTLENLPLPLVPQPNAATALAALRASGLEVSENA
                     IRDGIASAILPGRFQIVSESPRVIFDVAHNPHAAEYLTGRMKALPKNGRVLAVIGMLH
                     DKDIAGTLAWLKSVVDDWYCAPLEGPRGATAEQLLEHLGNGKSFDSVAQAWDAAMADA
                     KAEDTVLVCGSFHTVAHVMEVIDARRSGGK"
     gene            complement(25889..26803)
                     /gene="accD"
                     /locus_tag="J1Y83_000515"
     CDS             complement(25889..26803)
                     /gene="accD"
                     /locus_tag="J1Y83_000515"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HBA7628701.1"
                     /translation="MSWIERIKSNITPTRKASIPEGVWTKCDSCGQVLYRAELERNLE
                     VCPKCDHHMRMTARNRLHSLLDEGSLVELGSELEPKDVLKFRDSKKYKDRLASAQKET
                     GEKDALVVMKGTLYGMPVVAAAFEFAFMGGSMGSVVGARFVRAVEQALEDNCPLICFS
                     ASGGARMQEALMSLMQMAKTSAALAKMQERGLPYISVLTDPTMGGVSASFAMLGDLNI
                     AEPKALIGFAGPRVIEQTVREKLPPGFQRSEFLIEKGAIDMIVRRPEMRLKLASILAK
                     LMNLPAPNPEAPREGVVVPPVPDQEPEA"
     gene            complement(26959..27618)
                     /locus_tag="J1Y83_000516"
     CDS             complement(26959..27618)
                     /locus_tag="J1Y83_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HBA7628702.1"
                     /translation="MDLIYFLIDFILHIDVHLAELVAEYGVWVYAILFLILFCETGLV
                     VTPFLPGDSLLFVAGALASLETNDLNVHMMVVLMLIAAIVGDAVNYTIGRLFGEKLFS
                     NPNSKIFRRSYLDKTHQFYEKHGGKTIILARFVPIVRTFAPFVAGMGHMSYRHFAAYN
                     VIGALLWVLLFTYAGYFFGTLPFIQSNLKLMIVGIIFVSILPGVFEFIRHKRAAARAA
                     K"
     gene            complement(27701..28513)
                     /gene="truA"
                     /locus_tag="J1Y83_000517"
     CDS             complement(27701..28513)
                     /gene="truA"
                     /locus_tag="J1Y83_000517"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HBA7628703.1"
                     /translation="MSDQQQPPVYKIALGIEYDGSRYYGWQRQNEVRSVQEKLEKALS
                     QVANEPITVFCAGRTDAGVHGTGQVVHFETTAQRKDAAWTLGVNANLPGDIAVRWVKA
                     VPDDFHARFSATARRYRYIIYNHRLRPAVLSKGVTHFYEPLDAERMHRAAQCLLGEND
                     FTSFRAVQCQSRTPWRNVMHINVTRHGPYVVVDIKANAFVHHMVRNIVGSLMEVGAHN
                     QPESWIAELLAAKDRTLAAATAKAEGLYLVAVDYPDRYDLPKPPMGPLFLAD"
     gene            complement(28513..29526)
                     /locus_tag="J1Y83_000518"
     CDS             complement(28513..29526)
                     /locus_tag="J1Y83_000518"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HBA7628704.1"
                     /translation="MSEGWNIAVLGATGAVGEALLETLAERQFPVGEIYALARNESAG
                     EQLRFGGKTITVQDAAEFDWTQAQLAFFVAGKEATATWVEEATNSGCLVIDSSGLFAL
                     EPDVPLVVPEVNPFVLTDYRNRNVIAVPDSLTSQLLAALKPLIDQGGLSRISVTSLIS
                     ASAQGKKAVDALAGQSAKLLNGIPIDEEDFFGRQLAFNMLPLLPDSEGSVREERRIVD
                     EVRKILQDEGLMISASVVQAPVFYGHAQMVNFEALRPLAAEEARDAFAQGEDIVLSEE
                     NEFPTQVGDASGTPHLSVGCVRNDYGMPEQVQFWSVADNVRFGGALMAVKIAEKLVQE
                     YLY"
     gene            complement(29592..30728)
                     /gene="pdxB"
                     /locus_tag="J1Y83_000519"
     CDS             complement(29592..30728)
                     /gene="pdxB"
                     /locus_tag="J1Y83_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-phosphoerythronate dehydrogenase PdxB"
                     /protein_id="HBA7628705.1"
                     /translation="MKILVDENMPYARDLFSRLGEVTAVPGRPIPVAQLADADALMVR
                     SVTKVNESLLAGKPIKFVGTATAGTDHVDEAWLKQAGIGFSAAPGCNAIAVVEYVFSS
                     LLMLAERDGFSLHERTVGIVGVGNVGRRLQARLEALGIKTLLCDPPRADRGDEGDFRS
                     LDELVQHADILTFHTPLFKDGPYKTLHLADEKLIRSLKPGAILINACRGAVVDNTALL
                     TCLSEGQKLSVVLDVWEGEPELNVELLKKVDIGTPHIAGYTLEGKARGTTQVFEAYSK
                     FIGHEQHVALDTLLPAPEFGRITLHGPLDQPTLKRLVHLVYDVRRDDAPLRKVAGIPG
                     EFDKLRKNYLERREWSSLYVICDDASAASLLCKLGFNAVHHPAR"
     gene            30827..31822
                     /locus_tag="J1Y83_000520"
     CDS             30827..31822
                     /locus_tag="J1Y83_000520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulator Flk"
                     /protein_id="HBA7628706.1"
                     /translation="MIQPISGPPPGQPPGQGDNLPSGAGNQPLSSQQRTSLESLMTKV
                     TSLTQQQRAELWAGIRHDIGLSGDSPLLSRHFPAAEHNLAQRLLAAQKSHSARQLLAQ
                     LGEYLRLGNNRQAVTDYIRHNFGQTPLNQLSPEQLKTILTLLQEGKMVIPQPQQREAT
                     DRPLLPAEHNALKQLVTKLAAATGEPSKQIWQSMLELSGVKDGELIPAKLFNHLVTWL
                     QARQTLSQQNTPTLESLQMALKQPLDASELAALSAYIQQKYGLSAQSSLSSAQAEDIL
                     NQLYQRRVKGIEPRDMQPLLNPFPPMMDSLQNMATRPALWILLVAIILILVWLVR"
     gene            complement(31819..32997)
                     /locus_tag="J1Y83_000521"
     CDS             complement(31819..32997)
                     /locus_tag="J1Y83_000521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBA7628707.1"
                     /translation="MTAVSQTETRSSANFSLFRIAFAVFLTYMTVGLPLPVIPLFVHH
                     ELGYGNTMVGIAVGIQFLATVLTRGYAGRLADQYGAKRSALQGMLACGLAGGALLLAA
                     ILPVPAPFKFALLVVGRLILGFGESQLLTGALTWGLGIVGPKHSGKVMSWNGMAIYGA
                     LAVGAPLGLLIHSHYGFAALALTTMALPLLAWACNGTVRKVPALAGERPSLWSVVGLI
                     WKPGLGLALQGVGFAVIGTFVSLYFASKGWAMAGFTLTAFGGAFVVMRVMFGWMPDRF
                     GGVKVAIVSLLVETVGLLLLWQAPGAWVALAGAALTGAGCSLIFPALGVEVVKRVPSH
                     VRGTALGGYAAFQDIALGVSGPLAGMLATTFGYSSVFLAGAISAVLGIIVTILSFRRG
                     "
     gene            complement(33262..34482)
                     /gene="fabB"
                     /locus_tag="J1Y83_000522"
     CDS             complement(33262..34482)
                     /gene="fabB"
                     /locus_tag="J1Y83_000522"
                     /EC_number="2.3.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase I"
                     /protein_id="HBA7628708.1"
                     /translation="MKRAVITGLGIVSSIGNNQQEVLASLREGRSGITFSQELKDSGM
                     RSHVWGNVKLDTTGLIDRKVVRFMSDASIYAFLSMEQAIADAGLSPEAYQNNPRVGLI
                     AGSGGGSPRFQVFGADAMRGPRGLKAVGPYVVTKAMASGVSACLATPFKIHGVNYSIS
                     SACATSAHCIGNAVEQIQLGKQDIVFAGGGEELCWEMACEFDAMGALSTKYNDTPEKA
                     SRTYDAHRDGFVIAGGGGMVVVEELEHALARGAHIYAEIVGYGATSDGADMVAPSGEG
                     AVRCMKMAMHGVDTPIDYLNSHGTSTPVGDVKELAAIREVFGDKSPAISATKAMTGHS
                     LGAAGVQEAIYSLLMLEHGFIAPSINIEELDEQAAGLNIVTETTDRELTTVMSNSFGF
                     GGTNATLVMRKLKD"
     gene            34641..36647
                     /gene="mnmC"
                     /locus_tag="J1Y83_000523"
     CDS             34641..36647
                     /gene="mnmC"
                     /locus_tag="J1Y83_000523"
                     /EC_number="1.5.-.-"
                     /EC_number="2.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA
                     (5-methylaminomethyl-2-thiouridine)(34)-methyltransferase
                     MnmD/FAD-dependent
                     5-carboxymethylaminomethyl-2-thiouridine(34)
                     oxidoreductase MnmC"
                     /protein_id="HBA7628709.1"
                     /translation="MKHYSIQPANLEFNAEGTPVSRDFDDVYFSNDNGLEETRYVFLG
                     GNHLEARFPEHPHPLFVVAESGFGTGLNFLTLWQAFDQFREAHPQAQLQRLHFISFEK
                     FPLTRADLALAHQHWPELAPWAEQLQAQWPLPLPGCHRLLLDEGRITLDLWFGDINEL
                     TSQLDDSLNQKVDAWFLDGFAPAKNPDMWTQNLFNAMARLARPGSTLATFTSAGFVRR
                     GLQEAGFTMQKRKGFGRKREMLCGVMEQTLPLPCSTPWFNRTGSNKHEAAIIGGGIAS
                     ALLSLALLRRGWQVTLYCADEAPALGASGNRQGALYPLLSKHDEALNRFFSNAFTFAR
                     RFYDLLPVKFDHDWCGVTQLGWDEKSQHKIAQMLSMDLPAELAVAVEANAVEQITGVA
                     TNCSGITYPQGGWLCPAELTRNVLKLAQQQGLQIHYQYQLQDLSRKDDGWLLNFAGDQ
                     QATHSVVVLANGHQISRFSQTSSLPVYSVAGQVSHIPTTPELAELKQVLCYDGYLTPQ
                     NPANQHHCIGASYHRGSEETAYSDEDQQQNRQRLIDCFPHAQWAKEVDVSGKEARCGV
                     RCATRDHLPMVGNVPDYDATLVEYASLAEKKDEAVSAPVYDDLFMFAALGSRGLCSAP
                     LCAEILAAQMSEEPIPMDASTLAALNPNRLWVRKLLKGKAVKAG"
     gene            complement(36768..37046)
                     /locus_tag="J1Y83_000524"
     CDS             complement(36768..37046)
                     /locus_tag="J1Y83_000524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcL family protein"
                     /protein_id="HBA7628710.1"
                     /translation="MIAEFESRILALIDGMVDHASDDELFASGYLRGHLTLAIAELES
                     GEDHSAQAVHTTVSQSLEKAIGAGELSPRDQALVTDMWEHLFQQASQQ"
     gene            complement(37080..37628)
                     /locus_tag="J1Y83_000525"
     CDS             complement(37080..37628)
                     /locus_tag="J1Y83_000525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P hydroxylase"
                     /protein_id="HBA7628711.1"
                     /translation="MNSTHHYEQLIEIFNSCFADEFNTRLIKGDDEPIYLPADAEVPY
                     NRIVFAHGFYASAIHEISHWCIAGKARRELVDFGYWYCPDGRDAQTQSQFEDVEVKPQ
                     ALDWLFCVAAGYPFNVSCDNLEGDFEPDRVVFQRRVHAQVMDYLANGIPERPARFIKA
                     LQNYYHTPELTAEQFPWPEALN"
     gene            complement(37628..38437)
                     /locus_tag="J1Y83_000526"
     CDS             complement(37628..38437)
                     /locus_tag="J1Y83_000526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TSUP family transporter"
                     /protein_id="HBA7628712.1"
                     /translation="METFNSLFMVSPLLLGVLFFVAMLAGFIDSIAGGGGLLTIPALM
                     AAGMSPANALATNKLQACGGSISATIYFIRRKVVSLSDQKLNIAMTFVGSMSGALLVQ
                     YVQADVLRQILPILVICIGLYFLLMPKLGEEDRQRRMYGLPFALVAGGCVGFYDGFFG
                     PAAGSFYALAFVTLCGFNLAKATAHAKLLNATSNIGGLLLFILGGKVIWATGFVMLVG
                     QFLGARMGSRLVLSKGQKLIRPMIVIVSAVMSAKLLYDSHGQEILHWLGMN"
     gene            complement(38437..39261)
                     /gene="mepA"
                     /locus_tag="J1Y83_000527"
     CDS             complement(38437..39261)
                     /gene="mepA"
                     /locus_tag="J1Y83_000527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-insensitive murein endopeptidase"
                     /protein_id="HBA7628713.1"
                     /translation="MNKTAIALLALLASSASLAATPWQKITQPVPGSAQSIGSFSNGC
                     IVGADTLPIQSEHYQVMRTDQRRYFGHPDLVMFIQRLSRQVSNLGMGTVLIGDMGMPA
                     GGRFNGGHASHQTGLDVDIFLQLPKTRWTSAQLLRPQALDLVSRDGKHVVPALWKPEI
                     FSLIKLAAQDKDVTRIFVNPAIKQQLCLDAGTDRDWLRKVRPWFQHRAHMHVRLRCPA
                     DSLECEDQPLPPPGDGCGAELQSWFEPPKPGTTKPEKKTPPPLPPSCQALLDEHVI"
     gene            complement(39265..40350)
                     /gene="aroC"
                     /locus_tag="J1Y83_000528"
     CDS             complement(39265..40350)
                     /gene="aroC"
                     /locus_tag="J1Y83_000528"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HBA7628714.1"
                     /translation="MAGNTIGQLFRVTTFGESHGLALGCIVDGVPPGIPLTEADLQHD
                     LDRRRPGTSRYTTQRREPDQVKILSGVFEGVTTGTSIGLLIENTDQRSQDYSAIKDVF
                     RPGHADYTYEQKYGLRDYRGGGRSSARETAMRVAAGAIAKKYLAEKFGIEIRGCLTQM
                     GDIPLEIKDWSQVEQNPFFCPDPDKIDALDELMRALKKEGDSIGAKVTVVASGVPAGL
                     GEPVFDRLDADIAHALMSINAVKGVEIGDGFDVVGLRGSQNRDEITKDGFQSNHAGGI
                     LGGISSGQQIIAHMALKPTSSITVPGRTINRFGEEVEMITKGRHDPCVGIRAVPIAEA
                     MLAIVLMDHLLRQRAQNADVKTDIPRW"
     gene            complement(40385..41317)
                     /gene="prmB"
                     /locus_tag="J1Y83_000529"
     CDS             complement(40385..41317)
                     /gene="prmB"
                     /locus_tag="J1Y83_000529"
                     /EC_number="2.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3 N(5)-glutamine
                     methyltransferase"
                     /protein_id="HBA7628715.1"
                     /translation="MDKIFVDEAVNELQTIQDMLRWSVSRFSAANIWYGHGTDNPWDE
                     AVQLVLPSLYLPLDIPEDMRTARLTSSEKHRIVERVIRRVNERIPVAYLTNKAWFCGH
                     EFYVDERVLVPRSPIGELINNKFAGLISKQPQHILDMCTGSGCIAIACAYAFPEAEVD
                     AVDISPDALAVAEQNIEEHGLIHNVIPIRSDLFRDLPKVQYDLIVTNPPYVDAEDMSD
                     LPNEYRHEPELGLASGTDGLKLTRRILGNAADYLADDGVLICEVGNSMVHLMEQYPDV
                     PFTWLEFDNGGDGVFMLTKEQLLAAREHFAIYKD"
     gene            41483..42034
                     /gene="smrB"
                     /locus_tag="J1Y83_000530"
     CDS             41483..42034
                     /gene="smrB"
                     /locus_tag="J1Y83_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease SmrB"
                     /protein_id="HBA7628716.1"
                     /translation="MKKKTTLSEEDQALFRQLMAGTRKIKQDTIVHRPQRKKISEVPV
                     KRLIQEQADASHYFSDEFQPLLNTEGPVKYVRPDVSHFEAKKLRRGDYSPELFLDLHG
                     LTQLQAKQELGALIAACRREHVFCACVMHGHGKHILKQQTPLWLAQHPHVMAFHQAPK
                     EYGGDAALLVLIEVEEWLPPELP"
     gene            complement(42358..43215)
                     /locus_tag="J1Y83_000531"
     CDS             complement(42358..43215)
                     /locus_tag="J1Y83_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001357244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2544 domain-containing protein"
                     /protein_id="HBA7628717.1"
                     /translation="MKKTNLLFRLLILMVFMLGSRAGMGQTFRNVYIYYATFGWNTQI
                     ELYLDVAEVSSVYYGTYYQSNYVMTLGNLPNRTWTGTGSSPAPTITVKDGTTVNNSFC
                     PGMEALEAKDSRWECLKLPLDITVDGDVGGCPWLVSIRATSQVVVGSVDKDIYYGPNA
                     TVSVCPTVSVTPYDVSWDEYYINKTKTLSLQSTGGVIEKTLSTYLMKDGKLCDGSQMN
                     EEGAYCRWVAQMITFSSSGCDNANVTVAPNRHPITDKQLHDMVVRVDTSSRQPIDSTC
                     RFQYTLNEL"
     gene            complement(43217..43744)
                     /locus_tag="J1Y83_000532"
     CDS             complement(43217..43744)
                     /locus_tag="J1Y83_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001050113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HBA7628718.1"
                     /translation="MNLKRISHCAAALAAVLLTPHVQAADNLHFYGNLLSKSCTLVVQ
                     GESLAEVHFPTVSRKDLMVTGQSARVPVVFQLKDCKGPAEYEVRVTLTGTEDSEQPGF
                     LALDASSEAQGVGIGMEKTDGTPVLINNTSGATFVLSNGNNNLNFNAWLQAKSGREVT
                     MGEFTTSLTATFEYI"
     gene            complement(43741..44220)
                     /locus_tag="J1Y83_000533"
     CDS             complement(43741..44220)
                     /locus_tag="J1Y83_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001539250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HBA7628719.1"
                     /translation="MKRLAWCLIYGFAGLAQAAINDVTFHGTLVSPPACTISDGKTIE
                     VEFRNVIIDNINGDNFRQDVPYTITCDPDVRDDAWEMSLTWTGSQTPYDDSAIETDVR
                     GLGIELQQNGQPFKLGTPLKIDPSTPPTLQAVPVKANDAALSEGTFSAYATLQVDYQ"
     gene            complement(44217..44720)
                     /locus_tag="J1Y83_000534"
     CDS             complement(44217..44720)
                     /locus_tag="J1Y83_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000642898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HBA7628720.1"
                     /translation="MKAFFLTGSAALLLACGVTVFPAMAGGKIVNLTLRVLVDGPPPC
                     SVKGSAVEFGNVIIKSIDGANYRQPVGYTLNCSNSVSDDLQMQLQATTTTINGETVLS
                     TGISGFGIRIQNAADHSLVAVGNSSWLPFNINTQPDFEAVPVKQSGVELMASEFNATM
                     TMVVDYQ"
     gene            complement(44737..45489)
                     /locus_tag="J1Y83_000535"
     CDS             complement(44737..45489)
                     /locus_tag="J1Y83_000535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000120669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HBA7628721.1"
                     /translation="MSYKLSCPMLVSTALMAMLTTASLTAHASVTPDRTRLVFNESDK
                     SISVTLRNNNEKLPYLAQSWLEDEKGNKIISPLAVLPPVQRIDAMMNGQVKIQALPDI
                     HTLPSDRESLFYYNVREIPPKSGKANTLQIALQTRIKLFWRPKALEKIDMRRPWQFKV
                     TLTRSGQDYTVNNPTPYHVIISDASTQKKGLTAAGFKPLVMPPKTSQPLKAKMASAPV
                     LTYINDYGARMPLIFRCEGNTCKVDEDQSSKG"
     gene            complement(45509..>47924)
                     /locus_tag="J1Y83_000536"
     CDS             complement(45509..>47924)
                     /locus_tag="J1Y83_000536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001112832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="outer membrane usher protein"
                     /protein_id="HBA7628722.1"
                     /translation="YDINWYVSENDPTKNYACLTPELVAALGLKEGIAKSLQWTHNDE
                     CLKLGQLDGMEVENDLSQSALLLTVPQAYLEYTSSDWDPPSRWDDGIPGLIADYSLNA
                     QTRHQEQGGEDSHDISGNGTVGANLGAWRFRADWQSDYQHTRSNDDDDDSSNSTTSKH
                     WDWSRYYAWRALPSLKAKLSLGEDYLNSDIFDGFNYIGSSVNTDDQMLPPNLRGYAPD
                     VSGVAHSSAKVTISQMGRVLYETQVPAGPFRIQDIGDSVSGTLHVRVEEQNGQVQEYD
                     VTTASMPFLTRQGQVRYKVMMGRPEDWNHKTEGGFFSGGEASWGVADGWSLYGGALAD
                     KHYQSAAMGVGRDLAQFGALAFDVTHSHVNLDHDSAYGKGKLDGNSFRVSYAKDFDEL
                     NSRVTFAGYRFSEKNFMTMSEYLDANQSDMARTGNDKEMYTITYNQNFAAAGVSIYLN
                     YSHRTYWDRPEQTNYNLMFSHYFNMGSIRNMSISVTGYRYEYDDNADKGMYLSMSIPW
                     SDSSTVTYNGSYGSGSDSCQVGYFKRVDDATHYQVNVGTSEQHGSVDGYLSHDGSLAK
                     VDLSANYHEGEYRSAGIALQGGATLTAHGGALHRTQNMGGTRLLIDADGIANVPVESN
                     GAPVYTNMFGKAVVADINNYYRNQAYIDLNNLPEDAEATQSVVQATLTEGAIGYRKFK
                     VISGQKAMAVLRLRDGSYPPFGVEVKNDEQQQVGIVDDEGNVYLAGVNADEHMMVFWE
                     GSAQCEIVLPKPLPADLFSGLLLPCEQKGTAAPDSSAPEIKPVIQDQTRQVTPTEAPT
                     SISATQ"
BASE COUNT        11618 a        13027 c        12022 g        11257 t
ORIGIN      
        1 aaaacctttt tgaatattaa ataataatta attagcatca tccccattca ttaattccgc
       61 ttaacaatag tttcacgcct tgcccccatt gtgacacctc ggaaatcatc acgctgttaa
      121 actctccgta atgcggaaat atcatcatcg cgattcagta gtgaattctt aaacgaggca
      181 ttttcacaca attatcttac agataaaaaa accacactta caattaagaa tcagaacaat
      241 tcactacata acattactta ttaagcatat gcacctcatt attttgccat tattaagtta
      301 ttaacagcac aatcgagcct tcccctctgg caaaatctta ttctgcagac cttcaaaacg
      361 ccgtcctggg ggagtacatt gttctaagct gacttccacg gcagggagtg gcgataacag
      421 caaaaaaggt caagattcat gtcccccatt gaaaaatcca gcaaattaga gaatgtctgt
      481 tatgacatcc gtggtccggt gctgaaagaa gcaaaacgcc tggaagaaga aggtaacaag
      541 gtactgaaac tgaacatcgg caaccctgcc ccgttcggtt ttgacgcgcc agatgaaatt
      601 ctcgttgacg tgatacgcaa cctgcctacc gctcaagggt attgcgattc caaaggtctt
      661 tactccgcgc gtaaagccat catgcagcac taccaggctc gtggcatgcg tgatgttacc
      721 gtggaagata tttacatcgg caatggtgta tcggagctta tcgttcaggc aatgcaggca
      781 ttgctgaaca gcggggacga aatgttggtt cctgcacctg attacccact atggaccgca
      841 gcggtttcgc tctccagcgg taaagcggtg cattatcttt gcgatgaatc ctctgactgg
      901 ttcccggatc tcgatgatat tcgcgctaaa attacgcctc gtacgcgtgg gatcgttata
      961 atcaacccaa ataacccaac cggcgcggta tattccaaag agcttttaat ggagattgtg
     1021 gagattgcac gtcagcataa tctcattatc ttcgccgatg aaatttatga caaaattctc
     1081 tacgacgacg ctgagcatca ctccattgcg ccgctggcac ctgacctgct gaccattacc
     1141 tttaacggac tgtcgaaaac gtaccgcgtt gcaggcttcc gtcaggggtg gatggtgttg
     1201 aacgggccga aaaaacacgc caaaggttac atcgaaggtc tggaaatgct ggcttcaatg
     1261 cgcctgtgtg ctaacgttcc tgcgcaacac gccattcaga ccgcactggg tggttatcag
     1321 agcatcagtg aatttattac ccctggcggt cgtctttatg agcagcgtaa ccgcgcgtgg
     1381 gaattgatca acgatattcc gggcgtttcc tgcgtgaaac ctcgtggtgc gctgtatatg
     1441 ttcccgaaaa tcgacgccaa acgctttaac attcacgacg atcagaaaat ggtgctggat
     1501 ttcctgttgc aggaaaaagt cctgttggtg caagggacgg cattcaactg gccgtggccg
     1561 gatcacttcc gcattgtcac gctaccgcgt gtcgatgata tcgagctgtc tttgagcaag
     1621 ttcgcgcgtt tcctttctgg ttatcatcag ctgtaatctt aatttcactg ccggagattg
     1681 catccggcag tgttatcccg ccacaatgac ctgatgatgt catcatacgt aaggtcacta
     1741 tgaaacagag ccattttttt gcccatctct cccgcctgaa actcattaac cgctggccgc
     1801 taatgcgcaa cgtgcggacg gaaaatgtgt ccgaacacag tttgcaggta gcgatggtcg
     1861 cccatgcgct ggcagctatc aaaaatcgta aatttggcgg taatgtcaac gccgaacgta
     1921 tcgctttact ggcgatgtac cacgatgctt cagaagtgct caccggagat ctccctactc
     1981 cggtgaaata cttcaattcg caaatcgctc aggaatacaa ggctattgaa aaaatcgctc
     2041 agcaaaaact ggtcgatatg gttccggaag agctgcagga tatctttgct ccgttaattg
     2101 acgagcatgc atatagcgat gaagaaaaat cgctggtgaa acaggcagat gcactgtgtg
     2161 catatctgaa atgtctggaa gagctcgcgg ccggaaataa tgaattcttg ctggcaaaaa
     2221 cgcgactgga agcgacgctc gaagcgcgtc gcagccagga gatggactac ttcatggaag
     2281 tatttgttcc cagcttccat ctttcgctcg atgagattag ccaggattca ccgctgtaag
     2341 cagccggagt ctgcgtcgca tcaggcaata agcaccggat gcgacatcag gctcttgtca
     2401 aaacggaaac agcatcggga tcatcaccac acaaaccgcc atcacgataa tggtgaacgg
     2461 tacccccaac ttaacaaagt cactgaagct gtaattcccc ggacctaaaa ccagtgtgtt
     2521 aacaggtgaa gatatcggtg tcataaaggc ggcggatgct gccatcgcca cgaccatcgc
     2581 aaatggataa ggcgacaccc ccatcgtttt ggcagcagcc agcgcaatcg gagccatcaa
     2641 caccgccgtc gcggtattag aaataaatag cccaataacc gccgacaaga caaacaaaca
     2701 acccagcatc atatgtggcc cataaccgcc gccaatgtcc atcagccctt tcaccgccag
     2761 cgcgacacct cccgttttct gtaatgccac agcaaacggc atcatcccaa cgatcaaaat
     2821 aatgctcggc cagtgaatgg atttataggc actttcggca tcgatacagc ggaatttccc
     2881 catcagcagg caggcgatga tagcggcaac tggattagga atttcatcgg tcagcattaa
     2941 cgccaccatt agcaccagac agaaaatcgc atggggtgcc tggctgtgcg cgggcgatgc
     3001 ttcactcacc tcttccggta agttcagcgc tacgaagtcg cggccctgtt tggccagcat
     3061 accgatcagt ttccagttac ccacaaccag gatgatatcg cccagcagca gaggctcatc
     3121 cgccagcgaa ccttccagcg ccacgccatt gcgctttagc cccactacat tcaatccgta
     3181 gcgggtacga aaaccaattt cgcgcaccga tttaccaata agttctgact caggaattaa
     3241 tgaaatctct gccatgccca catcaagggc ctggtcagaa aaatactcgc cgcgcagtac
     3301 catcggctcc agcaattgct cactacaaaa ttgccggaga tcgacatcag ccgcagacat
     3361 atcaataagc aaaacgtcac gcgcgcgaaa ttcagaaacc ccattaacgt tcacgataac
     3421 gcgacgaaaa cgccgccagc gttcaacacc gatgacgtta gcaccataac gttcacgtaa
     3481 tttgagatcg tccagtcgtt gaccaatcat tggcgatccg gggcgaatag ccagacgtcg
     3541 cgcacgcccg gtcagtcgat attcacggat aagatcgcga aaggttcggc gcgtccagcc
     3601 ttcgcgttgt ggggtctggg tatccccttt cagcatgaaa cgcatcacta acatatacaa
     3661 aatacccagc accagcacga ccaggccaat aggtgttacg ctaaagaaac tgaagccgtg
     3721 atagccttca cgcagcaatt cactgttgac taccaggttc ggcggtgtcg ccaccagcgt
     3781 catcatgccg cttatgagcc cggcaaaact taacggcatc atcagacgcg acggcgacgt
     3841 ttgcatacgc atggcaacgc ttaacaccac gggaataaag atagcgacaa cgccggttga
     3901 actcataaac gcgccaagcc ccgcgacggt cagcatcaac aaaaccaaca ttttgatttc
     3961 actgttgccc gccactttga ccagccatgt ccccattaca gtggcaacac cggtacggac
     4021 caaaccatcg ccaataataa acaaggcggc aatcaggaca acgttaggat cggaaaagcc
     4081 ggaaaatact tcggggaccg tcagcgttcc gcttaatgca aacgcgacaa taacaaacaa
     4141 agcgaccgca tccatacgca ctctgcccgt cgcaaacaag acgatggcaa cggccagtaa
     4201 tgaaagaacc caaatcaatt caccgttcac aacttatcct tgttaattga gggggataac
     4261 ttgattctgc cataaaaaag ccccgacgag acggggctaa atcatgatca ggtgtttcac
     4321 tgaataataa catcgccatt tggctgtttg gtcacagtaa tttgctccag actgtggagg
     4381 actaaatcga cttcattcag gcgcggggta tctgccgggg cattaaccgc aataacatgg
     4441 caacccgccg ccagacctga aagcacgcca gcgggagcat cttccaccac aacacactcc
     4501 tgcggcgcaa gccccagcag ctgcgcgcct aacagatacg catcaggttc tggttttccg
     4561 cgcttcaccc gctcagcggt tacaaacacc tctggcgcgg gaagccccgc tattttatga
     4621 cgcgctcgcg ctaccggcat ggagccagaa gtcacaatag cccacggaat acctgcttta
     4681 ttcaaatgat tgagtaaggc gattgccccc ggaagcgcgg taataccttc ggtttccgtg
     4741 gcctcgatgt gctccagacg cgtaaactcg gcggcaatgt ctgcctcgga tttgcccgcc
     4801 ataaaatggc gcagagaggt gatcgcctgt ttaccgtgaa tgaaagccag cacctcttcc
     4861 ggcgataacc catgacgtct ggcccagttg ctccacgccc gttctaccgc aggcagggaa
     4921 tccaccagcg ttccatcaag atcaaacaga aaacctttgc accgcacgcg ggcctcctca
     4981 ggcgttaata atttgattaa tttcgttggc gctcaaatgg tactgacgcg ggcaggcatg
     5041 ccagacatta agcatgcgct gatatttttc ccacattggc gtctgggcgt taaaaccgtg
     5101 agttccagcg tcaaaatggg tatagcgccc ttccacatta accataaagc ggacataacc
     5161 gaggtaacgt gcttcagtgg cggcgtcaaa gccgaggaag gtgacacgac gttcatcgat
     5221 ggattgctgg tcctgcaaat tagaccagga aacatgcaac gcatgataca tctccataat
     5281 gtcgatgata gtgcggcagg tttcttcttt cagctcgcca aactcgcgat ccagttcacg
     5341 catctgtaat ccataaccac gctcaatgat tgtttgcagg cgacggtaac gttcagcatt
     5401 tgccggatcg agcatagtca tcatcttgta ctggttagac aaaataagac gttgcgcgtt
     5461 ggtcatttcc attattgact cctgtatcac tctactacgg tgaaaaaaaa gaaggctgag
     5521 tatgccttct tttatatgcg taatcagggg tcaattacaa atcatcaagg aaagttttat
     5581 ccagttgttt gaaggcacgc ttaagcgtgt cagctaatgc ctggtaatca ggcttgcctt
     5641 caacgggtgc caacacctgt ccagactcct gcaatttacc gcgaacttca taaaaccagt
     5701 tgaggattgc aggaggtaat ggcgttacag aacgcttgcc cagccaccac aatccctgca
     5761 tgggtaaact taaggcgaac agcgcagtgg caactgccgg cccaagctga ccgcccaggg
     5821 caatctgcca gcagagagta aatacggcga tcggcggcat aaaacggatc gcataacgcg
     5881 tcatcttgat aacgcgattt tcgacaaaga ctggggcaag gcgtttttcc agcggccacg
     5941 tctttgagta atgctgtccc cggcgaaaca agctaaaaaa attaacagaa cgattatccg
     6001 gcgttgacat gcttcacctc aacttcacat ataaagattc aaaaatttgt gcaaattcac
     6061 aactcagcgg gacaacgttc aaaacatttt gtcttccata cccactatca ggtatccttt
     6121 agcagcctga aggcctaagt agtacatatt cattgagtcg tcaaattcat atacattatg
     6181 ccattggctg aaaattacgc aaaatggcat agactcaaga tatttcttcc atcatgcaaa
     6241 aaaaatttgc agtgcatgat gttaatcata aatgtcggtg tcatcatgcg ctacgctcta
     6301 tggctccctg acgttttttt agccacgtat caattatagg tacttccatg tcgagtaagt
     6361 tagtactggt tctgaactgc ggtagttctt cactgaaatt tgccatcatc gatgcagtaa
     6421 atggtgaaga gtacctttct ggtttagccg aatgtttcca cctgcccgaa gcacgtatca
     6481 aatggaaaat ggacggcaat aaacaggaag cggctttagg tgcaggcgcc gctcacagcg
     6541 aagcgctcaa ctttatcgtt aatactattc tggcacaaaa accagaactg tctgcgcagc
     6601 tgactgctat cggtcaccgt atcgtacacg gcggcgaaaa gtataccagc tccgtagtga
     6661 tcgatgagtc tgttattcag ggtataaaag atgcagcttc ttttgcaccg ctgcacaacc
     6721 cggctcacct gatcggtatc gaagaagctc tgaaatcttt cccacagctg aaagacaaaa
     6781 acgttgctgt atttgacacc gcgttccacc agactatgcc ggaagagtct tacctctacg
     6841 ccctgccgta caacctgtac aaagagcacg gcatccgtcg ttacggcgcg cacggcacca
     6901 gccacttcta tgtaacccag gaagcggcaa aaatgctgaa caaaccggta gaagaactga
     6961 acatcatcac ctgccacctg ggcaacggtg gttccgtttc tgctatccgc aacggtaaat
     7021 gcgttgacac ctctatgggc ctgaccccgc tggaaggtct ggtcatgggt acccgttctg
     7081 gtgatatcga tccggcgatc atcttccacc tgcacgacac cctgggcatg agcgttgacg
     7141 caatcaacaa actgctaacc aaagagtctg gcctgctggg tctgaccgaa gtgaccagcg
     7201 actgccgcta tgttgaagac aactacgcga cgaaagaaga cgcgaagcgc gcaatggacg
     7261 tttactgcca ccgcctggcc aaatacatcg gtgcctacac tgcgctgatg gatggtcgtc
     7321 tggacgctgt tgtattcacc ggtggtatcg gtgaaaatgc cgcgatggtt cgtgaactgt
     7381 ctctgggcaa actgggcgtg ctgggctttg aagttgatca tgaacgcaac ctggctgcac
     7441 gtttcggcaa atctggtttc atcaacaaag aaggtacccg tcctgcggtg gttatcccaa
     7501 ccaacgaaga actggttatc gcgcaagacg cgagccgcct gactgcctga tttcacaccg
     7561 ccagctcagc tggcggtgct gttttgtaac ccgccaaatc ggcggtaacg aaagaggata
     7621 aaccgtgtcc cgtattatta tgctgatccc taccggaacc agcgtcggtc tgaccagcgt
     7681 cagccttggc gtgatccgtg caatggaacg caaaggcgtt cgtctgagcg ttttcaaacc
     7741 tatcgctcag ccgcgtaccg gtggcgatgc gcccgatcag actacgacta tcgtgcgtgc
     7801 gaactcttcc accacgacgg ccgctgaacc gctgaaaatg agctacgtcg aaggtctgct
     7861 ttccagcaat cagaaagatg tgctgatgga agagatcatc gcgaactacc acgctaacac
     7921 caaagacgct gaagtcgttc tggtggaagg tctggtcccg acacgtaagc accagtttgc
     7981 ccagtctctg aactacgaaa tcgccaaaac gctgaacgca gaaatcgtct tcgttatgtc
     8041 tcagggcact gatactccgg aacagttgaa agagcgtatc gaactgactc gcaacagctt
     8101 cggcggtgca aaaaacacca atattaccgg cgttatcgtt aacaaactga acgctccggt
     8161 tgatgagcag ggtcgtaccc gtccggatct gtccgagatt tttgacgact ccaccaaagc
     8221 aaaagtgaac aacgttgatc cggcgaagct gcaagaatcc agcccgctgc cggttctcgg
     8281 cgctgtgccg tggagctttg acctgatcgc gactcgtgcg atcgatatgg ctcgccacct
     8341 gaatgcgacc atcatcaacg aaggcgacat caatactcgc cgcgttaaat ccgtcacttt
     8401 ctgcgcacgc agcattccgc acatgctgga gcacttccgt gccggttctc tgctggtgac
     8461 ttccgcagac cgccctgacg tgctggttgc cgcttgcctg gctgccatga acggcgtaga
     8521 aatcggtgcc ctgctgctga ctggcggcta cgaaatggac gcgcgcattt ctaaactgtg
     8581 cgaacgtgct ttcgctactg gcctgccggt atttatggtg aacaccaaca cctggcagac
     8641 ttctctgagc ctgcagagct tcaacctgga agttccggtt gacgatcacg agcgtatcga
     8701 gaaagttcag gaatacgttg ctaactacat caacgctgac tggatcgatt ctctgactgc
     8761 cacttctgag cgcagccgtc gtctgtctcc gccagcgttc cgttatcagc tgactgaact
     8821 tgcgcgcaaa gcgggcaaac gtatcgttct gccggaaggt gacgaaccgc gtaccgttaa
     8881 agcagccgct atctgtgctg aacgtggtat cgcaacttgc gtactgctgg gtaatccagc
     8941 agagatcaac cgtgttgcag cctctcaggg tgtagaactg ggtgcaggca ttgaaatcgt
     9001 tgatccagaa gtggttcgcg aaaactatgt tggtcgtctg gtcgaactgc gtaagaacaa
     9061 aggcatgacc gaaaccgttg cccgcgaaca gctggaagac aacgtggttc tcggtacgct
     9121 gatgctggaa caagatgaag ttgatggtct ggtttccggt gctgttcaca ccaccgcaaa
     9181 caccatccgt ccgccgctgc agctgatcaa aactgcaccg ggcagctccc tggtatcttc
     9241 cgtgttcttc atgctgttgc cggaacaggt ttacgtttac ggtgactgtg cgatcaaccc
     9301 ggatccgacc gcagaacagc tggcagaaat cgcgattcag tccgctgatt ccgctgcggc
     9361 cttcggtatc gaaccgcgcg ttgctatgct ctcctactcc accggtactt ctggtgctgg
     9421 tagcgacgta gaaaaagttc gcgaagcaac tcgtctggcg caggaaaaac gtcctgatct
     9481 gatgatcgac ggtccgctgc agtacgacgc tgcggtaatg gctgacgttg cgaaatccaa
     9541 agcaccgaac tctccggttg caggtcgcgc taccgtgttc atcttcccgg atctgaacac
     9601 cggtaacacc acctacaaag cggtacagcg ttctgctgac ctgatctcta tcggaccgat
     9661 gctgcagggt atgcgcaagc cggttaacga cctgtcccgt ggcgcactgg ttgatgatat
     9721 cgtctacacc atcgcgctga ctgcgattca gtctgcacag cagcagtaat ctcgtcatca
     9781 tccgcagctt tgcgctgcgg atatctgaac cggaaatagt cactatttcc ggttttttat
     9841 tctcttaatt tgcattaatc ctttctgatt atcttgctta actgcgctgc atcaatgaat
     9901 tgcgccatct cactttgcat acttaccact ttgttttgtg caagggaata tttgcgctat
     9961 gtccgcaatc actgaatcta aaccaacaag aagatgggca atgcccgata cgttggtgat
    10021 tatctttttt gttgccattt taaccagcct tgccacctgg gtagttccgg tgggcatgtt
    10081 tgacagtcag gaagtgcagt atcaggttga tggtcaaaca aaaacacgca aagtcgtaga
    10141 tccacactca tttcgcattc tgactaacga agcaggcgaa cctgagtatc accgcgtaca
    10201 gctgttcacg acgggcgatg aacgcccggg tctgatgaac ttcccgtttg aagggttaac
    10261 ctcaggatcg aaatacggga cagccgttgg catcatcatg tttatgctgg tgattggcgg
    10321 cgcgtttggc attgtgatgc gtacaggaac cattgataac ggtatcctgg cgcttattcg
    10381 ccatacccgc ggaaatgaaa ttctctttat tcctgcgctg tttattctgt tttcacttgg
    10441 cggcgcaata tttggtatgg gagaagaggc cgtcgccttt gccattatca tcgcaccgct
    10501 aatggtccgg ctgggctatg acagtattac caccgtcctt gtgacctata ttgccacgca
    10561 aatcggtttt gccagttcgt ggatgaaccc gttttgtgtg gttgttgctc agggtattgc
    10621 cggcgttccg gtgctttctg gctccgggtt gcgcatcgtg gtgtgggtta tcgccactct
    10681 gattggcctg atctttacca tggtgtacgc ctcacgagtg aaaaagaatc ctcttctgtc
    10741 acgcgtgcat gagtccgacc gcttctttcg tgaaaagcag gcggatgttg aacaacgtcc
    10801 gtttaccttt ggtgactggc tggtattgat tgtcctgacc gccgtaatgg tctgggtgat
    10861 ttggggcgtg atcgttaatg cctggtttat tccagaaatt gccagccagt tcttcaccat
    10921 gggtctggtg attggcatca tcggcgttgt tttccgcctt aacggcatga cggttaatac
    10981 catggcttca tcctttaccg aaggggcgcg aatgatgatc gcccctgccc tgctggtggg
    11041 tttcgccaaa gggattttgc tgctggtcgg taatggtgaa gcgggtgatg ccagcgtgtt
    11101 aaataccatc ctcaacagca ttgccaatgc cattagcggt ctggataacg cggtcgcggc
    11161 ctggtttatg ttgctcttcc aggcggtatt taatttcttc gtgacgtccg gttctggtca
    11221 ggcggcgtta accatgccgt tactggcacc gcttggcgat ctggtcggtg ttaaccgtca
    11281 ggttaccgtg ctggcgttcc agtttggtga tggcttcagc cacatcattt acccgacctc
    11341 agcttcgtta atggcaacgc tcggtgtgtg ccgggtggac ttccgtaact ggctgaaggt
    11401 gggcgcgaca ctgcttggac tgctgtttat tatgtccagc gtcgtggtga tcggcgctca
    11461 gttgatgggc taccactaaa aatgttaaga gccgcaattg cggctctttt ttcattctgc
    11521 cgtttcagtc tctacggctt catttttggc attgcgcttc atccacaacg ccagcgcttt
    11581 cagcgagtct ggagtgaact catcgcagcg tgcggtgatt tcttccggcg tcagccagca
    11641 aacttcgctg acttcatctt cctgtagggc gaagggaccg tgagagacgc agctgaacaa
    11701 cgcgccccag acacggcaat ttttatcttc gaaatagaac tgcccgtgct cggcaaaggg
    11761 gacaccggca atgcccaact cttcttccgc ttcgcgacgc gcggattcca gcagttgctc
    11821 atcggcctgg accacaccgc ctgcggtcgc atctaacatg ccgggtaaaa agtcttttgt
    11881 ctcggtacga cgctggacca gaattttgcc catgccatca tgcacgacga tgtaagttgc
    11941 acgatgacgc agacactgtg cccgcatttg ttcccggctg gcttgtgcaa tgacttcgtt
    12001 ctcttcattg acaatatcca cccattcagt acttgccaaa cgacgctgtt ccattatcag
    12061 gaaaccttct ttttctggcg ctcttacggc gcattttgag ttgtgggtaa attacggatt
    12121 aatcgcgacc tgcgcaatga tactttgatc attgagtgcg attacactaa gtacgtcatt
    12181 atccagcatg ccataacttg ccggatgacc gcctttcgga atactcaccg agccggggtt
    12241 aaagtggaaa atctcccccc gctgttctgc cactggcaga tgggtatgac cgtacaccag
    12301 cacatcgttc tggtttaaag caggtaggtt ttccgggcca aaaagatgac catgcgtcaa
    12361 aaacagacgt tgtttttcca gtaatacctg ttgccacggc gcggttatcg ggaaatgcaa
    12421 aagcatttga tccacttcgc tgtcgcagtt gccgcgcaca gcgataacct tatgtgccac
    12481 ttcattaagc cgttcagcga ctttggctgg cgcgtaaccc tccggtaaag cattacgcgg
    12541 gccgtgattc aacacgtcgc caaggatcac cagccactgg gcaccacttt gggcaaacaa
    12601 ctccagaaca cgttccgtcg ccggtaacga cccatgaatg tccgatgcaa acatcagttt
    12661 catcactcac tcctcgtcga aaaaacgtaa gctatgatac tggattctgt cgttgctatc
    12721 agccagattg cttcgccgaa agagcaataa aacgctggac agacgctcgc tgccactgga
    12781 atgttgcata atccaccagg cgttctggca cctcatcgcc atgtagtacc aggcggttaa
    12841 tcattagcgc cagatcagta tcagcaatgc accattcacc aaataaattc ggctgaccca
    12901 gtgctaacaa atgttctgcc atcgcgaaca gtttctctgc actggctttt ccctcggccg
    12961 ttaatggcgc ttttttcgca cccgcaaaaa cgacatctgt cgaacgttct tcgcggatgg
    13021 gcatcagatc gctacgcagc caggcctgaa tctgtcgtgc acgcgcacga ttttctaaat
    13081 caagcggata aatgcgttcc caggtcggtg gcgcaaatcg atcttccaga tactccgcaa
    13141 tggcagaaga ttcactcaac tcaaaatcat cgatttgtaa taacggcaca cggcgtgtct
    13201 gaccgtaacc ttgccacgtc ggctgcaaat gttcaccgct gtcgaggtcg atggtcttga
    13261 tatgaaacga caggcctttt tcctgcaacg ccacccaggc ggataacaca taaggggaga
    13321 aaaaatgggc atctgaccaa agcgtgatag cgggtttact cattatgacc tcggcaataa
    13381 agggggctgg ctatcaaact atagagcctt tttgtcactg tcacgtgacg aatactcacg
    13441 caagtcgccg cgctttctat acttgtggtg ttcagatgat tagacaaccg gagctgcaat
    13501 gatcgatctc tatttcgccc cgacaccaaa tggtcacaaa attacgctgt ttctcgaaga
    13561 agcagggctg gattatcgct tgataaaggt agacctgggg aaaggcggtc agtttcgccc
    13621 ggaatttttg cacatttcgc ctaacaacaa aattccggca attgttgatc attcaccagc
    13681 cgatggcggc gaaccgctaa gcctctttga gtctggtgcc attttgttgt atctggctga
    13741 gaaaacagga ctctttttga gtcatgaaac gcgtgagcgc gccgccacat tacagtggtt
    13801 attctggcag gtaggcggac tggggccgat gcttgggcaa aatcatcatt ttaatcacgc
    13861 agcctcccaa accattcctt acgctattga acgttatcag gttgaaactc agcgtctgta
    13921 ctatgtactg aacaagcggc tggaaaactc gccctggctg ggtggcgaga actacagcat
    13981 tgcggatatt gcctgctggc cgtgggttaa tgcctggact cgccagcgaa ttgacctcgc
    14041 aatgtatccg gcagtcaaga actggcatga gcggatccgt tcgcgccctg ccaccgggca
    14101 ggcgctgcta aaagaacaac acggtgacga gcgttcggat agttaacaga aacaggttct
    14161 cgtgtattat ttcatcctaa gtaaaacaac ggagaacctg caatggcaca acctgccgct
    14221 attattcgta taaagaacct tcgtttacgt acgtttatcg gaattaagga agaagaaatt
    14281 aacaaccgtc aggatattgt tatcaacgtg gcgatccact accccgccga taaagcgcgc
    14341 actagcgaag atatcaacga tgcgctgaat tatcgcactg taacgaaaaa cattattcag
    14401 catgtagaga ataaccgttt ctctttgctg gaaaaattaa ctcaggatgt gctcgatatc
    14461 gcacgtgaac atcactgggt gacgtatgct gaagtggaga tcgataaact gcacgcgctg
    14521 cgctacgccg attcggtatc catgacctta agctggcagc gttaaccgcc atattgggag
    14581 gctgcatgaa catagtgatc accggaggga cgggattaat tggtcgccat ttgattccac
    14641 gtttgctgga cctgggccat caaatcacgg tagtgacgcg taacccgcag aaagccagtt
    14701 ccgttctcgg ccctcgggtg acactatggc aagggcttgc cgaacaaagc aacctcaacg
    14761 gcgttgatgc ggtaatcaac ctggccggag aaccgattgc tgataaacgc tggactcacg
    14821 agcaaaaaga gcgtctctgc caaagccgct ggaatatcac gcaaaaactg gtcgatttga
    14881 ttaatgccag cgacacgcca ccgtcggtac tcatttccgg ctcggcaacg ggctattatg
    14941 gcgacttagg tgaagtggtg gttaccgaag aggaaccgcc gcataacgaa tttacccata
    15001 aactctgcgc ccgctgggaa gaaattgcct gccgggcaca aagtgacaaa acgcgagtgt
    15061 gcctgctgcg taccggcgta gtgctggcac cggatggcgg tattctcggt aaaatgctgc
    15121 cgccgtttcg tcttggcctg ggcgggccga ttggctctgg tcggcagtat ctggcctgga
    15181 ttcatattga tgatatggtc aacggtattc tctggctgct ggataaagag ctgcgcgggc
    15241 catttaatat ggtttcgccc tacccggtgc gcaatgaaca atttgcccat gcgctcggtc
    15301 atgcgctgca tcgcccggcc attttgcgcg tccctgcgac cgccattcgg ctgttaatgg
    15361 gcgaatcttc agttctggta ttaggcggtc aacgcgcgct gccaaaaaag ctggaagaag
    15421 cgggttttgc gtttcgctgg tacgatttag aagaggcgct ggcggatgtc gttcgctgat
    15481 gtggtttact gcaaacatcc gccagttaac tcccggtgtt acagggttag tggctttgcg
    15541 cgataagatc gtctggtgaa agtcgggtca gcgtcaggat gacttcgtgg tcaaatcctt
    15601 tttccatcat ctcctgagca atgcgcaggg cttcttcgtg tttgccctgc attgcgcctt
    15661 cttcacgtaa tctgtcagca atggtcatca gtttctcctt ttcttgtggt gcgcgttccg
    15721 ctatctcacc aataaatgca cggaaacgcc gggcatcccc cgtttgtaat acgtaattaa
    15781 acagggcttt tagctgtctg tcattagtgt tccctgtaac tagcagcgaa acaatttggt
    15841 cgactaatcc caacagatcg cgctgacgaa tatgtttctg aattaactcc aacagcgcca
    15901 ttttgcgatg ttgcataatc tcgtcatccg gcaccacggt aatatccacc agcggaaaag
    15961 ccgatgaata aattttgcgg gcaatcgcag gctcggcaaa ttcatcaagc cagcagagtg
    16021 aatatggata agggcttctg caaccatgat aaaacagcat cgggatcact aatggaagct
    16081 ctttataacc cgcatcaaga tggttttgca tcgccgcaat ggaatagcgc atcatgcgaa
    16141 aagccattaa ttcctctggc ttactttggt gctctatcac cacataaata taacccgctc
    16201 cctcctgcgt tttcacagac cacaagaggt cggaataata ttgccgcagg tcttcatcaa
    16261 taaaactgtt tggttccagt ttaagcgtcg ttaaatcaca cagtttgcgc agcggcgcgg
    16321 gaagatgaat atcaataaaa tcccgcgcgg tgtctggatg gcgtaaaaaa gatttaaata
    16381 ccgcatcatg cggcgtggaa gttgtcgata ttgtcatggc gttccgtcac ccctcaaatt
    16441 taagatgacg cgacaataac cacgatttac acccaatgca gcaggcagtt tttatctttg
    16501 gacagcgaat tcagaactat ttttagcgga ctgcaacttt tacatttcag cggaagcgga
    16561 ctcgcaaatt ccccatgaaa tactccggca gcgttaacca ccggagagga aattatttca
    16621 gcgaaccttt aaggaactgt tgcagacgag ggctttgcgg gttaccgaat aactgctccg
    16681 gcgccccttc ttcttctatt ttcccctgat ggaggaaaat gacgtgagta gaaacatgac
    16741 gagcaaagcc catttcgtga gtcactacca ccatggtttt cccctcttct gccagttgct
    16801 gcataatacg caacacttcg cctaccagtt caggatcgag cgctgaagta ggttcatcaa
    16861 acagtaaaac ttccggttcc atcgctagcg cccgcgcgat agaaacacgc tgttgctgac
    16921 caccggacag gtgtaccggg tatttcccct gcgcacgttc gtctatcccg acttttgcca
    16981 gatatttcac cgcccgctcg cgcgcttcct gcttgctcag gcccaacacc tgaatcggcg
    17041 cttccatgac gttttccagc accgtcatat ggctccagag attgaagtgc tggaatacca
    17101 tcgtcaggcg tgtgcgcaat aagcgcagct gatttttatc ggcgactttg agttgaccgt
    17161 ctttatcgcg caccagattg atcgtctggc cattgaccac gatcgatcct tcgctcggtt
    17221 tttcgaggaa gttaatgcag cgcagaaagg tacttttacc cgatcccgac gatccgatga
    17281 tgcttatgac atctccggca ttcgcttgca atgagacccc tttcagcact tcgtgttcgc
    17341 cgtagcgttt gtgcaaatcg ataacgttta atttattctc ggacatcgta ttctcagtgc
    17401 gttgaagaag gtttcacatg ctgcaaccag cgtttttcag ctctacgaaa gaggctgatc
    17461 aggacataag agatgattaa atagagcacc gcggcaatgc cgaaagcggt aaaaggttga
    17521 tatgtggcgg cgttaatatc gcgggcaatt ttcagcagat ccggcaccgt ggcagtaaat
    17581 gccagcgcag tagagtgcag catcaggatc acttcgttgc tgtacgccgg taacgcaata
    17641 cgcagcgcag aaggcaaaat aatgcagcga tacattttaa aggtcgagaa gccataggct
    17701 ctggcggctt caatttcccc atgcggcact gaacggattg ccccggcaaa aatctcggta
    17761 gtgtaagcgc aggtgttaag cgtcagcgcc agcacggtac agttcagacc actgcgaaaa
    17821 aaggcgttaa ggaactcggt tcccttaaca atctccagcg tgtacatgcc ggaatagaac
    17881 accagcaact gaacatacag cggcgtaccg cgaaaaatat aggtaaataa ccagattgga
    17941 aactggatgt atttattact tgagacgcga ccaatcgcca gaaacagcgc cagaactccg
    18001 cctatcacta ccgacaaaat cagcagccac agggtgatcg ccacgccagt aaagcgataa
    18061 ccgtcagtcc acagcagtgg tttccagtat tcatgtaaga tttcgatcac aggtcagccc
    18121 tcttcacacc cacggagtag cggcgctcaa ggaacagcag cacaccattg gaaacggtgg
    18181 tgaaaacaag ataaatcacg ccacagacga tagcgaaata gaacggttcc caggtacttt
    18241 tgcctgccag ttgcgtggct ttgaccacat cttccaggcc gagtaacgaa accagtgcgg
    18301 tagatttgag gatcacctgc cagttgttgc caatgcctgg cagggcgtaa cgcatcatcg
    18361 ccggaaacat gatccgccga aacacttgcc cacgagtaaa accgaacgcc gtcgccgcct
    18421 ctatatgtcc tttcggcact gccataaacg cgccacgaaa cgtttcggta aaataagcac
    18481 cgtagataaa accgagagtg ataatacctg cgaccatcgg atcaatatca atctgcccga
    18541 cgcccatcgc ctccgtcacc gtatttagcg caatctgcaa accgtagaaa atcagcagca
    18601 tcaatactaa atccggcacg cctcgaatca gcgtggtgta cccttcgaaa ataaggcccg
    18661 aaagccgatt ttgtgagagc ttaccgccag cgccaattaa accgatgatt acagcgagca
    18721 ctacagagct gatagccagc tccagcgtga cgagcgcacc ctgtaaaata acacctgaaa
    18781 acccatacaa catgctgcct gtcctgtcgt gtgtggtgaa ttaccgcctc ttgtctccct
    18841 ccggataccc gaagggagcg ggctttcttt caccgatggc gattaaccac cataaacatc
    18901 aaaatcgaag tactttttcg ctaatttctc gtaagtaccg tcagcgcgca tttcggcaaa
    18961 ggctttgttc agtgcctcgc gcagttcgtt atcttcttta cgcaggccca tgccggtccc
    19021 tacgccaaac agtttttcat ctttaacaga cgggccaccg aatttgtaat ctttaccgac
    19081 gggttgtttg aggaaacctt cgctggctgc gacctcatcc tggaacgcgg catcaatacg
    19141 tccggcagtc aggtcagaat aaatattgtc ctgcccttga taagaaacga tttcaatgcc
    19201 ttttggtgcc cagtgttcgt taccgaacgt ctcctgggtg gtgccctgca atacgcctac
    19261 ccgtttgcct ttcagcgatt cgactgtcgg ctgaatgtcg gaatttttcg ccaccaccag
    19321 acgagaatcg gctgcgtaca gtttgtcggt gaaggctatt tcttgctgac gtttttccgt
    19381 aatggaaagc gatgacatga tggcgtcaat cttcttcgct tttaaggacg ggattaacgc
    19441 atccagtggg ttttcgacaa acgtacattg cgtattgatg cgtttgcaca actccttcgc
    19501 cagatcgata tcgaagccaa ccagttcacc ttgtgaattt tttgattcaa atggcgcata
    19561 ggtcggatcg gtaccgatgc ggatgttttg cggaatcgca gcaaatgccg cagttgcgct
    19621 ggagaaggcc agaaccagag agagcgatag caccagtttt ttcataatgt cctcaactga
    19681 cagtcttttt atcgggaata tttacaggtt tgttacactt atcgtgccat aaagttgacc
    19741 atcaaggcaa aatatcccgc cccgccagac gtattatttt tcattaaaaa tgcttaagta
    19801 tgcatttcaa tgcaccacga tagtgcaaca aacctggcgt gcaccatgat gatcattcca
    19861 tcaggtacag cttccctgcg acgtatcagt caccgtagac attaaagtcg aaatactttt
    19921 tcgccatctt gtcgtaggtg ccgtcctgac gcagctcgtt aagcgcctta ttgaaggccg
    19981 ccgtcagttc agcatcatct ttacgcagcc ctacaccggt gccgtcaccg aagtattttt
    20041 tgtctttcac tgatgggcct gcaaaggcga aatctttacc ggcgggttgc ttaaggaaac
    20101 cttcgctggc agcaacttca tcttgtaacg cagcatccag acgtcctgca gccagatcgg
    20161 aatagactaa atcctggttg gcataggcca ccacatccac gcctttgcta cgccaggtct
    20221 cgttagcgta tgcttcctgg gttgatccct gcagcacacc aacatgttta cctttcagtg
    20281 aatccagcgt tggctgaatc ggtgaacctt tggcggcaat caaacgagaa tctgcggcgt
    20341 acagcttgtc agagaaggct atctcctgct gacgtttatc ggtaatggag agcgacgaaa
    20401 taatggcgtc gatttttttc gctttcagtg aggggatcag cgcgtcaaag tcgctggcaa
    20461 cccaggtaca tttcacctgc atccgtttgc acatctcgtt accgagatcg atatcaaagc
    20521 caacaaagtc acctttagca tctttcgatg agaacggtgc gtaggtggta tcggttccga
    20581 tacgtaccgt ctccggtagc gccgcatagc tggaagccgc tgtggagaga ccgactaaca
    20641 aagacagagc gagaatcgac ttcttcatac ataaccctca agtgaaaatg gcttttttat
    20701 gttgtgtatt gtgttgtgtg tttgcaggct ctttcatgca ggtcttatgc catcttgacg
    20761 acagcagtaa cattcaacgt taaatatgtt aataagacgt tgcattattg tcctaaagtt
    20821 gaagatagca ggtatagcga ttgaatcgca gcgtttcgat tgaatggcag aaacaaaatg
    20881 tcgaggattt gatcgcggtt gcaaaattgc cctgaaacag ggcaacagcg gagttatgcg
    20941 ccctgccagc gggcaaagag atcttcagga agggttaccg caaactggtc aagaacacga
    21001 ttaaccgtct gatttatcac atcatcaagg gattgtgggc gatgataaaa cgccggaacg
    21061 ggaggcataa tcaccgcacc gatttctgcc gcctgagtca ttaaacgcag atggcctaag
    21121 tgcagtggcg tttcacgcac gcagagcacc aacgggcgac gttctttcag caccacatct
    21181 gccgcacggg tcagtaagcc atcagtatag ctatggacaa tgccggacag ggttttgatt
    21241 gaacagggta aaatcaccat ccccaacgtc tggaaagaac cggaagaaat gctggcagca
    21301 atatcgcgcg catcgtgtgt cacatcagct aacgcctgca cttcgcgcag ggaaaaatcc
    21361 gtttcgaggg ataaggtctg gcgcgccgcc tggctcatta ccagatgcgt ttcgatatct
    21421 gtgacatcgc gcagaacctg taataaacgc acgccataaa tcgcgccgct ggcaccgctg
    21481 atgcctacaa tgagtcgttt cataaatagt tgccctgtca gacttgcagg cagactttgc
    21541 aggatttcgg cgggagttgc aagtcagggt gccagaccgg caccctgagc gaaggcatca
    21601 tccttcgtta tgcatttcga gattttccac ttcgttctga cgttgcactg ctttggcgtc
    21661 gtcattacgt aaagtatcga ggaaatcgag gtagccctga tcaacgtctt tggtgacgta
    21721 gacgccgttg aacaccgagc attcaaactg ctggatatcc gggttttcag cgcgaacggc
    21781 ttcgatcaga tcgttcagat cctggaaaat caacccgtca gcaccgatga tctggcgaat
    21841 ttcatctact tcgcgaccgt gagcgatcag ttccgtggcg ctcggcatat cgataccgta
    21901 aacgttcggg aagcgaattt ccggtgccgc agaagcgagg tacactttct tcgctccggc
    21961 ttcgcgtgcc atctcgataa tctgctcaga agtggtgcca cggacgatag agtcgtcgac
    22021 cagcaggacg tttttatcgc ggaactcggc gcggttggcg ttcagtttac ggcgcacgga
    22081 cttacgacgc agctgctgac ccggcatgat gaaggtgcgg ccaacatagc ggtttttaac
    22141 gaagccctgg cggtacggtt tgcccagaat acgcgcaatt tccagcgcga tatcgcagga
    22201 agtttccggg atcgggatca ccacgtcgat atccagatct tcccattcgc gggcaatttt
    22261 ctcgcccagc ttcgtgccca tattcacacg cgcgctgtaa acggaaattt tgtcgatgaa
    22321 cgagtccggg cgagcaaagt atacatactc aaacaggcac ggattgctga ccggattgtc
    22381 agcacactga cgggtaaaca actgcccttc ttcagtgatg taaatcgctt cgccaggggc
    22441 gacatcacgc aggaactcaa agcccagcgt atcgagcgca acgctttcgg aagcgaccat
    22501 atattctgtg cgattatcgt caatatcacg tttgcccagt acaagcggac gaatcccgtt
    22561 aggatcgcgg aaagcaacca taccgtggcc gataatcatc gccacacagg catacgcgcc
    22621 gcggattaag cggtttgtag cagcgatggc ggcgaaaatg ttgtcggctt ccagcgggta
    22681 gtggcggaag ttgtccagct cgctggcgaa gatattaagc agaatttccg agtcggaagt
    22741 ggtgttgatg tggcggcgtt tttcttcaaa cagtttttta cgcaactcgt gagcgttggt
    22801 cagattgccg ttgtgggcaa gcgtaatgcc atacggggag ttaacgtaaa acggctgcgc
    22861 ttcagaggcg ctggagctgc cagccgtggg gtaacgcaca tgaccaatgc ccatattgcc
    22921 ctgcaaacgc tgcatatggc gagcttcaaa tacatcgctc accagcccgt tcgctttacg
    22981 caaacggaag cagttattgg catctatggt gatgatgccg gcggcatcct gaccgcgatg
    23041 ctgaagcacc gttaaggcat cataaatcga ctggttaacc ggcataacac cggcgatacc
    23101 gacaataccg cacatacgtc tttttcctcg ttaagccaca tctcagagca cttacgctct
    23161 gggcaagaaa cttgacgagc tttgcagata atcaaaaaac catctgatga taaaactgaa
    23221 ctgcgggatc agctgtgatt tgctccagtc ttcgcttttc gacaccccgg taaaggagtc
    23281 gagaaagaag agaatggcag caacaatcaa cacaccgcgc aacgcaccga aacagacgcc
    23341 caacacccga tcggtgcctg acaaccccgt tttctccacc aactggccta tcacgaagtt
    23401 cacgatagca ccaacgatca gggtagcgat aaacagtacc gcgatggcaa tcccatttcg
    23461 aaccagttcg tcttcaaagc ccgtaaacca gactgacagg taagtgtagt aatgactggc
    23521 aacaaagaaa gcacaacccc atgtcaccag cgataacgct tcacgaacaa agccgcggat
    23581 caggctaacc agagaggaaa aagcaatcac cgcgattatg gcgtaatcaa tccagaccat
    23641 atgtgtccca cgattttacg ccctgtcatc ctgttcgggg cgcattctaa cagaaaaaga
    23701 aaacgtttgc gtagggattt ccttcccgcg catcaataaa aatggcgctg aaaaaatatt
    23761 caacgccatc gactttttat gcctttgcgg catcgggcaa tgcgtgtcgg atgccgcatg
    23821 aacgccttat ccgacctacg gctctacccc tgcgcaggcc tgataagacg cgccagcgtc
    23881 gcatcaggca agaccgtatt aattcggcgt gtagcccatt accacgccac ttaagccaga
    23941 aagttgcttc aactcaccca gcgatccttt cagcttatct ttcgaggcat ctggcccaac
    24001 cagaatacgg gtaattttac cctgcactgg cgtggatggc gacgtataaa cccgataacc
    24061 ggcaccgcgc agcttaccga caatctcatt cactttatcg gcatttttca gcgcgcccag
    24121 ttgcacaaca taggctttac ccgttggcgc tgctttttct tccacgaccg gcttcggttc
    24181 tggcttcggc gcaggtggtg cttcaacctt cggcttaggc ggctccaccg gtttcggctt
    24241 cggtgggacg accggtgcag gttccggttc aaactcggtg ttattggctg caatagtggc
    24301 cggatcgagc gacggtgctg cggcatcacc tgcccgcacc tcttccgctg cgccttccgg
    24361 cggctgcgtc ggcaacgcct gggtggcggc tggcatcata tcaggttcat cgcgatcgcc
    24421 cgcttttggc accagcggga tagccgcgaa ctcatcctga taatgttttt tctgcccgtc
    24481 cagcagccct ggaagtacaa tcacccccag cgccaccagc acgatcgtgc ccactaaccg
    24541 attctgaaac ttacttgcca ccgcttctcc tcgcgtcaat cacttccatg acatgtgcga
    24601 ccgtgtggaa cgaaccacac accagcacgg tatcttccgc tttagcgtcc gccattgcgg
    24661 catcccatgc ctgcgccacg ctatcaaatg atttgccgtt acccaaatgc tcaagcagtt
    24721 gttctgccgt ggcaccgcgc ggcccttcca gtggcgcaca ataccagtca tcaaccacgc
    24781 ttttcaacca ggccagagtt ccggcaatat ctttatcatg tagcataccg ataaccgcca
    24841 gcacgcgccc gtttttcggt agcgctttca tacgcccggt gagatattcc gccgcatgtg
    24901 gattatgcgc gacatcaaaa ataacgcgtg gcgactcgct cacaatctgg aaacgtcccg
    24961 gcaaaattgc gctggcaatc ccgtcgcgaa tggcattttc actgacctcc agcccgctgg
    25021 cacgcagtgc cgccagcgcc gttgcggcat tcggttgcgg gacaagcggc aacggcagat
    25081 tttccagcgt gccgtgagca tcgctaaacg tccagtcatg atcggtgacg gaatagttcc
    25141 actcaacgcc ccgacgttgt agcagtgcac ctttttcctg cgccacatca gcaatggtag
    25201 aaggcatctc cggctcaccg acaattgccg gtttttcgct gcgaaagata cctgctttct
    25261 cgcgaccaat actttcgcga tctggcccca gccagtcggt atgatccagc gcaatactgg
    25321 ttactaccgc gacatcggca tcgacaatat tggttgcgtc cagacgaccg cccagcccta
    25381 cttccagaat cactacgtca agttgtgcct gcttgaacag ccgcaacgcc gacagcgtac
    25441 cgtattcgaa ataggtcagg gaaatatcac cgcgtgccga ttcaatctcc gcaaaagaag
    25501 cggtgtgggc cgattccggc aattcctgac cctgcacacg tacgcgctcg gtataacgca
    25561 ccagatgcgg cgaactgtag acgcccactt tgtaccctgc cgccatcaga atcgactcca
    25621 gcgtacggca ggtggtgcct ttgccattag tacccgcaac ggtaaacaca aatggcgctg
    25681 gtttcagaac gccaagacgc gccgcgacct ggctcacgcg ctcaaggccg agatcgatag
    25741 ttttactgtg caggttttcc agataagaaa gccacgaagc cagaggcgac gcggcttgag
    25801 gagtgcgttt gataatcatg gtatccgctg attcgttacg gtgagaatag caaaagggca
    25861 gagccagtgg ccctgccctt atcagttatc aggcctcagg ttcctgatcc ggtaccggtg
    25921 gcaccactac gccttcacgc ggcgcttcag gattcggcgc tggcagattc atcaacttcg
    25981 ccagaatgct cgccagtttc aggcgcattt ccggacgacg gacgatcatg tcgatcgcgc
    26041 ccttctcgat caggaattca ctgcgctgga atcctggcgg cagtttttcg cgaacggtct
    26101 gttcgataac tcgcggaccg gcaaagccaa ttaacgcttt cggttcagcg atgttgagat
    26161 cgcccagcat ggcgaaactt gcagaaacac cgcccatggt cgggtcggtc agcacggaga
    26221 tgtacggcaa gccgcgctcc tgcatttttg ccagtgccgc agaggttttc gccatctgca
    26281 tcagcgacat cagtgcttcc tgcatacgtg cgccaccaga ggcggagaag cagatcagtg
    26341 ggcagttatc ttccagcgcc tgctcaacgg cacgcacgaa acgcgcacct accacagatc
    26401 ccatggaacc gcccataaag gcgaactcga atgccgcagc gacaaccggc attccataca
    26461 gagtgccttt catcaccacc agcgcatctt tttcgccggt ttctttctgc gcagatgcca
    26521 gacggtcttt atacttcttg gagtcacgaa acttcagcac atctttcggc tcaagctcgc
    26581 tacccagctc cacaaggctt ccttcatcta acaggctatg caggcgatta cgcgctgtca
    26641 tacgcatgtg atggtcacac ttcggacaga cctcaagatt acgttccagc tcagcgcggt
    26701 ataaaacctg accgcagcta tcacacttag tccacacccc ttcaggaatg ctcgccttgc
    26761 gggtgggagt aatgttgctt ttaattcgtt caatccagct cattagggac ctttctgtct
    26821 gaacctggtt cgatgccagt tttatctttg gggacgcata atgccatttt tgcccccaac
    26881 agaccatgaa tgttgcacat taaaacataa cagcccgaaa ctttggataa aaaagtggtc
    26941 gaaccgcaga gttacttttt attttgcggc gcgtgccgca gcgcgtttgt ggcgaataaa
    27001 ttcgaaaaca cctggcaata ttgaaacaaa aataatgcca acaatcatca gtttaaggtt
    27061 gctctgaatg aaggggagcg tgccgaagaa atagcctgcg taggtaaaaa gcagtaccca
    27121 caacagtgcg ccaatcacgt tataagcggc gaaatgacgg tacgacatgt ggcccattcc
    27181 cgcaacaaac ggggcgaacg ttctgacgat cggcacaaaa cgggcgagaa taatcgtttt
    27241 gccgccatgt ttctcataaa actgatgggt tttgtcgaga taactgcgac ggaaaatttt
    27301 cgaattggga ttactgaata acttttcacc gaacaaccgc ccgatagtgt aattgaccgc
    27361 gtcacccaca atcgcggcga tcagcatcag caccaccatc atatggacgt tgagatcgtt
    27421 ggtctccagt gatgccaacg ctccggcaac aaacagcaat gaatcacccg gtaaaaacgg
    27481 cgttaccacc agaccggttt cacagaacaa aattaagaac aaaatggcat aaacccagac
    27541 gccgtactcc gcgaccagct ctgccagatg cacgtcaatg tgcaggataa aatcgatgag
    27601 gaaataaatc aggtccatat ttgcctatgc cttgtactcg tcatctatca ggctgtaact
    27661 acgttggcta cagcctgaaa tgttccgagc attattctcg ttagtccgcc agaaatagcg
    27721 ggcccattgg cggttttgga agatcatatc ggtcagggta atccaccgcg accagatata
    27781 gcccttccgc ttttgccgtt gctgccgcca gcgttctgtc ctttgccgcc agcaactctg
    27841 ctatccagct ctccggctgg ttgtgggcac cgacttccat caggctgccg acaatattcc
    27901 tgaccatatg atgtacaaag gcattcgctt tgatatctac caccacataa ggaccgtgac
    27961 gcgtgacatt aatatgcata acgttgcgcc acggggtccg ggactggcac tgcaccgcac
    28021 ggaacgaggt gaaatcattc tcgcccagca agcattgcgc agcccgatgc atccgttcag
    28081 cgtccagcgg ttcgtaaaaa tgggttaccc ctttactcaa taccgccggg cgcagccgat
    28141 gattgtagat gatgtagcga taacggcgag ccgtggcgct aaatcgggca tgaaaatcat
    28201 caggtacagc tttaacccaa cgcacggcga tgtcaccagg taaattcgca tttacgccca
    28261 acgtccacgc agcgtctttg cgctgcgcgg tcgtttcgaa atgcaccacc tgcccggtac
    28321 cgtgtacacc ggcgtcagta cgcccggcgc agaagacggt gatgggttcg ttcgccacct
    28381 gggagagcgc cttttccagc ttctcctgca cactgcggac ttcattctgc cgttgccagc
    28441 cgtaatacct actgccgtcg tactcaatgc ccagcgcaat tttataaact ggcggttgtt
    28501 gctggtcgga cattagtaca gatactcctg caccagtttc tcggcgattt ttactgccat
    28561 cagcgcgccg ccaaagcgaa cgttatcggc caccgaccag aactggactt gctccggcat
    28621 accgtagtca ttacgtacgc agccaacaga aagatgcggc gtaccagaag catcccctac
    28681 ctgagtcggg aattcgttct cttcagagag cacaatatct tcgccctgag caaacgcatc
    28741 acgcgcttct tctgccgcca gcggacgcag tgcttcaaag ttgaccatct gggcatgacc
    28801 gtagaatacc ggtgcctgga cgacgcttgc cgaaatcatc agcccttcgt cctgcaggat
    28861 tttgcgtact tcgtcaacga tacgacgttc ttcacgcacg ctaccttcgc tatccggcag
    28921 taacggcagc atgttgaacg ccagctgacg cccgaagaaa tcttcttcgt caatcggaat
    28981 gccattgagt aatttagcac tctgccccgc taacgcatcg accgcttttt taccctgggc
    29041 ggaggctgaa atcaggctgg taacgctgat acgtgataaa ccgccctgat caattagcgg
    29101 tttcagtgcc gccagcagct ggctggtcag actgtctggt acggcgatga cattccggtt
    29161 gcggtaatcg gtcagtacaa acgggtttac ttccggcacc accagcggta cgtcgggttc
    29221 gagagcaaac aatccgctgc tgtcgatcac cagacaacct gagttggttg cttcttcaac
    29281 ccaggtagcg gtagcttctt tgcctgcgac aaaaaacgcc agctgcgcct gcgtccagtc
    29341 gaattcagcg gcatcctgca cggtgattgt cttaccacca aagcgcagtt gttctcctgc
    29401 gctttcgtta cgtgccagtg cataaatttc cccaaccggg aactgacgtt cagccagcgt
    29461 ttcaagcagg gcttcgccca cagcgccagt tgcgcccagg acggcaatgt tccagccttc
    29521 agacatggtg gtttactcca gaaatagcaa agctccctgc caatgttaca gcagagagca
    29581 tgaagaagag attaacgtgc cggatgatga acggcgttaa aacccagttt acacagcaat
    29641 gatgccgcac tggcgtcatc acaaatcaca tacagagatg accattcacg gcgctcaaga
    29701 tagtttttgc gcagtttatc gaactcaccc ggtatcccgg cgactttacg cagcggtgcg
    29761 tcatcgcggc gcacatcata caccaaatgc accagccttt tcagcgttgg ctgatcgagc
    29821 gggccatgca gcgtaatgcg accaaactct ggcgcgggca gtaatgtatc cagcgcaacg
    29881 tgctgttcat gcccaataaa cttgctataa gcttcaaaca cttgcgtagt accgcgtgct
    29941 ttaccttcca gggtatagcc tgcgatatgc ggcgtgccga tatccacttt tttcagcaat
    30001 tccacgttaa gttccggttc gccttcccag acatccagca ctacgcttaa cttctggcct
    30061 tcgctcaggc aggtcagcag ggcggtatta tcgacgactg cgccacggca ggcgttaatc
    30121 aggatcgctc ccggtttcag gctgcggatc agtttttcat ccgccagatg cagcgttttg
    30181 tacggaccat ctttaaagag tggcgtatgg aaagtcagaa tatccgcatg ctgaactaat
    30241 tcatccagcg agcggaaatc cccctcatcc ccacggtcgg cgcggggcgg atcgcaaagt
    30301 aaggttttga tccctaaagc ttccagtcgc gcctgtaaac gacgaccaac gttaccaacg
    30361 cccacgatcc ccacggtacg ctcgtgcagt gaaaatccat cgcgttcggc aagcatcagc
    30421 agggaggaga aaacatattc cactaccgca atcgcattac agccaggtgc agcggaaaaa
    30481 ccaattcccg cctgctttaa ccatgcttca tcgacatggt cggtccccgc ggtggcagtg
    30541 ccaacaaatt taatgggttt tcctgccagc aaagattcat tcactttcgt gaccgaacgc
    30601 accataagcg catccgcgtc tgccagttga gcgacaggaa ttggacgccc gggaaccgcg
    30661 gtcacctcac ccaaacggct aaataagtcg cgggcataag gcatattttc atcaacaagg
    30721 attttcacgt ttgtgttacc tgtatgagac gagagttaac cagacaagtg tgccataatc
    30781 tcgcggccag gcatacttgc gaagatttca ggtataagga tacgtaatga tacaacctat
    30841 ttccggccct cctcctgggc aaccaccagg tcagggagat aacctgccgt ctggcgcagg
    30901 caatcagcct ttatccagtc agcaacgtac ttcgctggaa agcttaatga cgaaagtgac
    30961 ctcactgacg caacaacaaa gagcagaact gtgggcgggt atcaggcacg atattggtct
    31021 gtcgggagat tcaccgctgc tttcgcgtca cttccctgcc gctgagcata atctggcgca
    31081 acgtctgttg gccgcgcaaa aaagccattc tgcccgccag cttttagcgc aattagggga
    31141 gtatttacgt ctggggaata atcgtcaggc ggtcacggat tatatccgtc ataactttgg
    31201 tcagacgccg ctgaatcagc tctcaccgga gcaattaaaa accattctca ccctgttgca
    31261 ggaagggaag atggttattc cgcaaccaca gcagcgcgag gcgaccgacc gtcctttatt
    31321 accggcggag cacaatgcgc tcaaacagct ggtgaccaaa cttgcggcag caacggggga
    31381 acccagcaaa cagatctggc aatcgatgct ggaactttcc ggggtgaaag atggcgagtt
    31441 aattccagcg aaactgttta accatctggt gacctggcta caggcgcgtc agacgctaag
    31501 ccagcaaaat acgccgacac tggaatcact acagatggcg ctaaaacaac ctttagatgc
    31561 cagtgaactg gcggcgttat cggcatatat ccagcaaaaa tatggccttt ctgcgcaatc
    31621 atcgctttct tctgcccagg ccgaggatat tcttaaccag ctttatcaac ggcgggttaa
    31681 agggattgag ccgcgtgata tgcaaccgct gcttaatcct tttccaccga tgatggactc
    31741 gttgcaaaat atggcaacgc gtcccgcgct gtggatactg ttagtcgcga ttatcctgat
    31801 tctggtctgg ctggttcgtt aaccccgacg aaacgacagt atcgtgacaa taattcccag
    31861 caccgcagag atcgccccgg caagaaatac cgaagagtaa ccaaacgtgg tcgccagcat
    31921 tcccgcaagc ggcccggaga cgccgagggc gatatcctga aacgcggcgt aaccgcccag
    31981 tgcggtgccg cgaacgtgtg aggggacgcg tttaaccacc tccacgccca gcgcaggaaa
    32041 gataagcgaa catccggctc cagttaatgc cgcgcctgct aatgcgaccc acgcccctgg
    32101 ggcttgccag agcagcaaca agcccaccgt ttctacaagc agagagacga tcgccacttt
    32161 cacgccgcca aaacggtccg gcatccagcc aaacatgacg cgcatcacga caaatgcgcc
    32221 gccaaacgcg gtaagagtaa agcccgccat cgcccatcct ttgctggcaa agtagagcga
    32281 aacgaaagtc ccgataaccg caaaaccaac gccttgtagt gccagaccta accctggttt
    32341 ccagataagc ccgacaacgc tccacagcga tggacgttcc cccgccaggg ccggtacttt
    32401 gcgcactgtg ccgttacagg cccacgccaa taagggtaat gccattgtgg tgagcgccaa
    32461 tgcggcaaaa ccgtaatggc tatgaatcaa caggccaagc ggcgcaccaa cagcgagggc
    32521 accgtaaata gccattccgt tccatgacat cactttgccg gaatgttttg gcccaacgat
    32581 gcctaatccc caggtcagag cgcccgtcag taactggctt tcaccaaagc caagaatcaa
    32641 acgcccgacg accaacagag caaatttgaa cggtgcgggg acgggcaaaa tcgccgccag
    32701 cagtaacgcg ccccccgcca gaccacaagc taacatcccc tgaagcgccg aacgttttgc
    32761 gccatattga tcggccagtc gcccggcgta accacgcgtc agcaccgtag ccagaaactg
    32821 aatcccgacg gcaatgccga ccatagtgtt gccatagccc agttcatgat gaacaaacag
    32881 cgggataacc ggcaacggta aacctacggt catgtaggtg agaaaaaccg caaaagcgat
    32941 gcggaagagc gaaaaattgg cagaagatcg tgtttcggtt tggcttacag cagtcatgca
    33001 ttactccaga atgcagcgca aggcgaggag tatccccgtc tcatctctct ggtttcaggg
    33061 ttacggtgcg ttggcaggat ttaacgcgta cgtcttttct gaaggaaatc gacaaagcgg
    33121 gaagtttgcc tggaactggc ggcgattgtc aatgatgtga aaaagggaac catcgggttc
    33181 ccttttgcgt tagtgccgga ggccgcattg gcgcgtaacg tcggatgcag cgctggcgcg
    33241 tcttatccga cctactccga attaatcttt cagcttgcgc attactagcg tggcgttagt
    33301 gccgccgaag ccgaagctgt tagacataac tgtggtcagt tcgcgatcgg tcgtttcggt
    33361 cacgatgttc agacccgcag cctgctcgtc cagctcttca atgttgatgc tcggggcgat
    33421 aaaaccgtgt tccagcatca gcagagagta gatagcttcc tgtacgccag cagcgcccag
    33481 tgagtgaccg gtcattgctt tggttgcaga aatcgccggg ctcttatcgc cgaacacttc
    33541 acggatagct gccagttctt tcacgtcgcc aaccggagtc gaagtaccat gagagttcag
    33601 gtagtcgatt ggggtatcaa caccgtgcat cgccatcttc atgcagcgca ctgcaccttc
    33661 gccagacgga gcaaccatgt ctgcgccatc agaggttgcg ccgtagccaa cgatttcagc
    33721 atagatgtga gcaccacgcg ccagcgcgtg ttccagctct tcaaccacta ccataccgcc
    33781 accgccagcg ataacgaaac cgtcacggtg agcgtcgtaa gtacgggagg ctttttccgg
    33841 ggtgtcgttg tatttagtag acagcgcacc catcgcgtcg aattcgcaag ccatttccca
    33901 gcacagctct tcgccgccgc cagcaaacac gatgtcctgt ttgcccagtt ggatctgctc
    33961 tactgcgtta ccgatacagt gtgcggaagt cgcacacgcg gagctgatgg agtagttaac
    34021 gccgtgaatt ttaaacggcg tggcgaggca ggcagaaacg ccggatgcca tcgctttggt
    34081 gaccacatac gggccaaccg ctttcaggcc gcgcgggccg cgcattgcgt cagcgccgaa
    34141 aacctggaaa cgcggggaac cgccgccgga acctgcaatc aggccaacgc gcgggttatt
    34201 ctggtaagct tccggggaga ggccagcatc tgcaattgcc tgctccatag aaaggaatgc
    34261 ataaatggat gcgtcgctca taaagcgcac aactttgcgg tcaatgaggc cagtggtatc
    34321 cagttttacg ttgccccaga cgtggctacg catgccggaa tccttcagct cctgagagaa
    34381 agtgatccct gaacgtcctt cacgcagaga tgccaggact tcctgctggt tattaccgat
    34441 gctggaaaca atgcccaggc cagtaatcac tgcacgtttc attcaatacc tctgtaagtc
    34501 gcacatagag taagtttcga atgcacaata gcgtacactt gtacgccgaa caagtccgat
    34561 cagccaatta atagagaaat ttgcgcagcc tgacacacat cgctaagatc gagccaccgc
    34621 ctgtaagacg agtaacttac gtgaaacact actccataca acctgccaac ctcgaattta
    34681 atgctgaggg tacacctgtt tcccgagatt ttgacgatgt ctatttttcc aacgataacg
    34741 gactggaaga gacgcgttat gtttttctcg ggggtaacca tctagaggca cgttttcctg
    34801 aacatccaca tcctctgttt gtggtagcag agagcggctt cggcaccgga ttaaacttcc
    34861 tgacgctatg gcaggcattt gatcagtttc gcgaagcaca tccgcaagcg caattacaac
    34921 gcttacattt cattagtttt gagaaatttc ccctcacccg tgcggattta gcgctggcgc
    34981 atcaacactg gccggagctg gccccgtggg cggaacagct tcaggcgcag tggcctttgc
    35041 ccctgcccgg ttgccatcgt ttattgctcg atgaaggccg cataacgctg gatttatggt
    35101 ttggcgatat aaacgaactg accagccaac tggacgattc gctaaatcaa aaagtagatg
    35161 cctggtttct ggacggcttt gcgccagcga aaaacccgga tatgtggacg caaaatctgt
    35221 ttaacgccat ggcccgactg gcgcgtccgg gcagcacgct ggcgaccttt acgtctgccg
    35281 gttttgtccg ccgcggtttg caggaagccg gattcactat gcaaaaacgt aagggctttg
    35341 ggcgcaaacg ggaaatgctt tgtggggtga tggaacagac attacccctc ccctgctcca
    35401 cgccgtggtt taaccgtacg ggcagcaaca aacatgaagc ggcaattatt ggaggcggta
    35461 ttgccagcgc gttgttgtcg ctggcgctat tgcgtcgcgg ctggcaggta acgctttatt
    35521 gcgcggatga ggctcccgca ctgggtgctt ccggcaatcg ccagggggcg ttgtatccgt
    35581 tattaagcaa acacgatgaa gcgctaaacc gttttttctc taatgcgttt acttttgctc
    35641 gtcggtttta cgatttattg cccgttaaat ttgatcatga ctggtgcggc gtcacgcagt
    35701 taggctggga tgagaaaagc cagcataaaa tcgcacagat gttgtcgatg gatttacccg
    35761 cagaactggc tgtagccgtt gaggcaaatg cggttgaaca aattacgggc gttgcgacaa
    35821 attgcagcgg cattacttat ccgcaaggtg gctggctgtg tccagcagaa ctgacccgta
    35881 atgtgctgaa actggcgcaa cagcagggtt tgcagattca ttatcaatat cagttacagg
    35941 atttatcccg taaggatgac ggttggttgt tgaattttgc aggagatcag caagcaacac
    36001 acagtgtagt ggtactggcg aacgggcatc aaatcagccg tttcagccaa acgtcatctc
    36061 tgccagtata ttcggtggcc ggtcaagtca gccatattcc gacaacgccg gaactggcgg
    36121 agctgaagca ggtgctgtgc tatgacggtt atctcacgcc acaaaatccg gcgaatcagc
    36181 accattgtat tggtgccagt tatcatcgcg gcagcgaaga gacggcgtac agtgatgaag
    36241 atcagcagca gaatcgccag cggttgattg attgtttccc acacgcgcaa tgggcaaaag
    36301 aggttgatgt cagtggtaaa gaggcgcgct gcggtgtgcg ttgtgccact cgcgatcatc
    36361 tgccaatggt aggcaacgtg cctgattatg acgctacact cgtggaatat gcgtcgctgg
    36421 cggagaagaa agatgaagcg gtaagcgcgc cggtttatga cgatctcttt atgtttgctg
    36481 ctttaggttc gcgcggtttg tgttctgccc cgctgtgtgc cgagattctg gcagcacaga
    36541 tgagtgaaga accgattccg atggatgcca gtacgctggc ggcgttaaat ccgaataggt
    36601 tatgggtgcg gaagttgttg aaaggtaaag cggttaaggc ggggtaatct gctttggcat
    36661 tgtttgtcgg atgcggcgta aacgccttat ccgacctacg cgttacctgt aggcctgata
    36721 agacgcgata agcgtcgcat caggcatggt gctccagacg tcacaactta ctgctgtgaa
    36781 gcctgctgaa acaggtgttc ccacatatcg gttaccagcg cctggtcacg cggtgacaat
    36841 tcacccgcgc caatggcttt ttccagactc tggctaacgg ttgtatgtac cgcctgagcg
    36901 gagtggtcct caccactttc cagttctgcg atggctaatg tcaggtggcc acgcaaatac
    36961 ccactggcaa acaactcatc atcactggca tggtcaacca taccgtcgat taatgccaga
    37021 atgcgtgatt caaactccgc gatcatcttc tttcctcatt gtaaaacgtg gcgctggctt
    37081 cagttgagcg cttccggcca cgggaactgt tccgccgtaa gttccggcgt gtgataataa
    37141 ttttgtagtg ctttaatgaa gcgtgccgga cgttcgggaa tgccgtttgc cagataatcc
    37201 atcacctgcg catgtacccg ccgttgaaac accacgcggt ccggttcgaa atccccttcc
    37261 agattgtcgc agctaacatt aaacggatat cccgctgcca cgcagaacaa ccagtccagc
    37321 gcctgcggct tcacttcaac atcttcaaac tggctttgtg tctgggcatc gcgtccgtcc
    37381 gggcaatacc agtagccgaa atcaaccagc tcacggcgcg ctttcccggc aatacaccag
    37441 tgcgaaatct cgtggatggc gctggcataa aagccatgag cgaagacgat tctgttatac
    37501 ggtacttccg catcagcagg aagatagatc ggttcgtcgt cgcctttaat cagacgggta
    37561 ttaaattcat cggcaaagca gctattaaaa atctcaatca actgctcata gtggtgtgta
    37621 ctgttcatta gttcatcccc aaccagtgga ggatctcctg tccatggcta tcgtaaagta
    37681 atttggcact catcaccgcc gagacgataa cgatcatcgg gcggatcagc ttttgtcctt
    37741 tgctcaacac cagtcttgaa cccatgcgcg cgccgaggaa ctgccccacc agcatcacaa
    37801 agcccgtcgc ccaaatcact ttgccgccga gaataaacag cagcagaccg ccaatgtttg
    37861 acgttgcgtt aagtaatttg gcgtgagcag tggctttggc gaggttgaat ccgcacagcg
    37921 taacgaaggc cagcgcgtaa aacgatccgg cagccgggcc aaagaatcca tcgtaaaaac
    37981 cgacgcagcc ccccgccacc agcgcaaatg gcagtccata catccgacgc tggcgatctt
    38041 cttcacccag ctttggcatc agcaagaaat agaggccgat acaaatcacc agaataggca
    38101 aaatctgccg taagacatcg gcctgaacat actgcaccag tagcgcgccg ctcattgagc
    38161 cgacaaaggt catggcgata ttgagttttt gatcgcttaa actaaccact ttacggcgaa
    38221 taaagtagat agtggcggaa atagagccac cgcaggcttg cagtttattg gttgccagcg
    38281 cgttagcggg agacatcccc gccgccatca atgccggaat ggtgagtaac ccaccgccac
    38341 cggcaatcga gtcgataaac cccgccagca tggcgacaaa aaagagaact cccagcagca
    38401 gcggggaaac cataaacagg ctattaaacg tttccattag atcacgtgct catccagtag
    38461 cgcctggcag gaaggcggca acggaggcgg tgtcttcttc tcaggctttg ttgttcccgg
    38521 ttttggaggt tcaaaccagc tttgcagttc tgccccgcaa ccatcgcctg gtggcggtaa
    38581 aggttgatcc tcacactcca gactatcggc aggacaacgt aatcgtacat gcatatgcgc
    38641 gcgatgctgg aaccagggtc gcactttgcg caaccagtcg cgatcggtgc ccgcatcaag
    38701 gcaaagttgt tgcttaatcg ccggattaac aaaaatgcgc gtgacgtctt tatcctgggc
    38761 ggcgagtttg atcaagctga aaatttctgg cttccacagc gcggggacaa cgtgtttacc
    38821 gtcgcgggaa actaaatcta acgcttgcgg gcgcaagagc tgcgcggagg tccagcgtgt
    38881 tttcggcagt tgcaggaaga tatcgacatc cagtccggtc tggtggctgg cgtgaccgcc
    38941 gttgaaacga ccaccagcag gcatccccat atcgccaatc agcaccgtac ccatgcccag
    39001 attgctcacc tggcgactca gacgctggat aaacatcacc aggtctgggt gaccgaaata
    39061 gcgacgctga tcggtacgca tgacctgata atgttcggac tgtatcggca acgtgtcagc
    39121 gccgacgata cagccattag aaaaactgcc tatcgattgt gcgctacccg gcacaggttg
    39181 ggttattttt tgccacggcg tcgctgccag gctggcacta ctggcaagca gggccagcag
    39241 cgcaatcgca gttttgttca ttttttacca gcgtggaata tcagtcttca catcggcatt
    39301 ttgcgcccgc tgccgtaaca ggtgatccat taaaacgatc gccagcatcg cttctgcgat
    39361 cggcaccgcg cggatcccaa cacaaggatc gtgacggcct ttggtgatca tctcaacttc
    39421 ttcgccaaag cggttaatag tgcgccccgg cacggtaatg ctggaggttg gcttcagcgc
    39481 catatgggca atgatttgct gcccgctgct gataccacca agaatgccgc ccgcatggtt
    39541 gctctggaaa ccgtctttgg tgatttcgtc gcggttctgg ctgccgcgta gccccaccac
    39601 gtcaaaaccg tcgccaattt ccacgccttt caccgcgttg atgctcatta gcgcatgggc
    39661 gatgtcggca tccaggcggt caaagaccgg ctcgccaagt ccggcgggta cgccactggc
    39721 aacgacggtg actttcgcac caatagagtc gccttctttt ttcagcgcgc gcatcagttc
    39781 atctaacgcg tcgattttgt ccgggtccgg gcagaagaat ggattttgct cgacctgaga
    39841 ccagtctttg atttccagtg gaatgtcgcc catctgggtc aggcaaccgc gaatctcaat
    39901 gccaaatttc tcggcgagat attttttggc aatcgcccct gccgccacgc gcatagcggt
    39961 ttcacgggcg gaagaacgtc caccgccgcg ataatcacgc aggccgtatt tttgttcgta
    40021 agtgtaatcg gcatggcctg gacggaagac gtctttaatt gcgctgtaat cctgagaacg
    40081 ctggtcggta ttttcgatca acaagccaat gctggtgcca gtagtaacac cttcaaaaac
    40141 accggagaga attttgacct gatccggctc acgacgctgg gtggtatagc gcgatgtccc
    40201 agggcgacga cgatcaaggt catgttgcag gtccgcttcc gtcagcggaa tgcctggcgg
    40261 aacaccatcg acgatgcagc cgagcgccag cccgtgcgat tcgccgaagg tggttacgcg
    40321 aaagagttgt ccaattgtgt ttccagccat cacggctccg ttattgttgt gtttgcgtgt
    40381 ttacttaatc tttataaatc gcgaaatgtt cacgggccgc cagcagctgc tctttggtga
    40441 gcataaacac accatcgccg ccgttatcaa actccagcca ggtgaacgga acatccggat
    40501 attgttccat aagatgtacc atgctgttgc ccacttcaca aatcagcaca ccatcatcag
    40561 cgaggtaatc tgccgcgtta ccgagaatgc gacgcgtcag tttcaggccg tcggtgccag
    40621 atgccagacc gagttccggc tcgtggcggt attcgtttgg caggtcggac atatcttccg
    40681 catcgacgta cggcgggttg gtgacgatca ggtcgtactg cactttcggc aagtcacgga
    40741 acaagtcgga acgaatcgga atgacgttgt ggatcaaacc gtgttcttcg atgttctgtt
    40801 cagcaaccgc cagcgcgtct ggagagatgt ccaccgcgtc gacttccgct tccgggaagg
    40861 cataagcaca ggcaatggcg atgcagccgc taccggtaca catatctaaa atatgctgcg
    40921 gttgcttgct gatgagtccg gcaaatttat tgttgatcag ttcaccaatc ggcgagcgcg
    40981 gcaccagcac gcgttcatcg acgtaaaatt catggccgca gaaccaggct ttgttggtca
    41041 ggtaagccac cggaatgcgc tcgttgacgc ggcggatcac gcgttcaaca atacggtgtt
    41101 tttcgctgga ggtcagacgc gcggtgcgca tgtcttccgg aatatccagc ggcaggtaga
    41161 gcgaaggcaa caccagttgc acggcttcat cccacgggtt atcggtgccg tggccgtacc
    41221 agatattcgc cgcgctgaag cggctcaccg accagcgcaa catgtcctga atggtttgca
    41281 gctcatttac tgcttcatca acgaaaattt tatccacgta ttcctccagg gcatgatcgc
    41341 aataatttcg gcggctagtt tgccatgaag atgacgataa atcagcattc acacgcggtg
    41401 agtgaggaaa aatacgtttt aagcgatcga ttgtacggtg agtcgggtaa actgtaggaa
    41461 aattaaaaat agagacagat aaatgaaaaa gaaaacaaca cttagcgagg aggaccaggc
    41521 gctgtttcgc cagttgatgg cgggtactcg caagattaag caggacacga ttgtccaccg
    41581 accgcagcgt aaaaaaatca gcgaagtacc agtaaagcgg ttgattcagg aacaggccga
    41641 tgccagccac tatttttcgg acgaatttca gccattattg aataccgaag gtccggtcaa
    41701 atatgttcgc ccggatgtca gccattttga ggcgaagaag ttacgccgtg gcgattattc
    41761 accggagttg tttcttgatt tgcacggtct gacgcagcta caagccaaac aggaactggg
    41821 ggcgttgatt gccgcctgcc gccgtgaaca tgtgttttgc gcctgcgtga tgcatgggca
    41881 cgggaagcat attttgaagc agcaaacgcc gctgtggctg gcgcaacatc cgcatgtgat
    41941 ggcatttcac caggcaccaa aagagtatgg tggcgatgcg gcgttgttgg tgctgattga
    42001 agtggaagag tggttgccgc cggaattacc ctgaatatta aaaggtggat gagtcgggcc
    42061 atttagcctt gaatactggc gtctcaaccc tggcaataat gaacgtaata atcgggcatt
    42121 aattgaaaaa cgtaagcagc tttccgacag ccttctgtgg gctaatggca ttgccccttc
    42181 agcggggctt gaggatgacg acctgctgca agcgcttatc gacgaattac tcacccccgt
    42241 cgacggttat ctggcacttt tcgctcccgt ggtggcgaat attctaaaaa actggatgac
    42301 cgcatgacat aacggacaac ggggccagat ggccccgcat cgtgtgtggt gtatacgcta
    42361 cagctcgttg agcgtgtact ggaagcggca ggtggaatca atcggctggc ggctactggt
    42421 atcgacacgc actaccatgt cgtgcagttg cttgtcagtg atgggatgcc tgtttggcgc
    42481 caccgtaaca ttagcattat cacagcctga tgatgaaaac gtgatcattt gcgcaaccca
    42541 gcgacagtaa gcgccctctt cattcatttg cgagccatca catagtttgc cgtctttcat
    42601 cagataggtt gaaagcgttt tttcaattac tccgcccgtc gattgcaaag acagtgtttt
    42661 cgttttattg atatagtatt catcccatga aacatcatac ggcgtaactg acacggttgg
    42721 gcacacggac acggttgcat ttggtccata atagatatct ttatcaacag aaccgaccac
    42781 aacctggctg gtggctctga tactcactaa ccacgggcag cctcccacat caccatcgac
    42841 ggtaatatcc agaggcagct tgagacattc ccagcggctg tctttggctt ccagtgcctc
    42901 catgccagga caaaatgaat tatttacagt tgtcccatct tttaccgtaa tcgtcggcgc
    42961 aggagaactg cccgttcctg tccatgtgcg attgggtaag ttgccaagcg tcataacata
    43021 attactttga taataggtac cgtagtaaac actcgaaacc tcagcaacat caagatacag
    43081 ttcgatctga gtattccagc caaacgtagc atagtagata tacacattac gaaatgtctg
    43141 ccccatcccg gcccgactac cgagcataaa aaccatcagg atcaaaagac gaaacagcaa
    43201 gttagttttt ttcatcttat atgtactcaa acgttgcggt taaggaggtg gtaaactcac
    43261 ccatcgtcac ttcccggccg cttttcgcct ggagccaggc attaaaattc agattgttat
    43321 tgccgttact cagcacaaaa gttgccccgc tggtattgtt aatcagcacc ggtgttccgt
    43381 cggttttttc catgccaatt ccgacacctt gcgcctctga agaagcatcc agcgccagaa
    43441 aaccgggctg ttcgctgtct tccgtgccag tcaacgtaac ccggacttca tactcggctg
    43501 gccctttaca atctttcaac tgaaacacta ccggcacccg cgccgactgc ccggtcacca
    43561 tcaggtcttt gcggctaacg gtggggaaat gcacctccgc cagcgactcg ccctgaacga
    43621 ctaacgtgca ggacttgctg agtaaattgc cgtagaaatg caggttgtca gccgcctgaa
    43681 catggggcgt cagcagcacc gccgccagtg cagccgcgca gtgagatatc cgttttaggt
    43741 tcattggtaa tccacctgta gtgttgcgta ggcagaaaag gtgccttcgc tgagcgccgc
    43801 atcattggct ttcaccggta cggcttgcag cgttggtgga gttgacgggt caattttcaa
    43861 cggtgttccc agcttaaacg gctggccgtt ttgctgtagc tcaatcccca ggcccctaac
    43921 gtccgtttca atggcggaat catcataagg ggtttggctg cccgtccagg tcaggctcat
    43981 ctcccaggcg tcgtcgcgca catcggggtc acatgtgatg gtgtaaggca catcctgacg
    44041 gaaattatcg ccgttaatgt tgtcgataat gacgttgcga aactcaactt cgatcgtctt
    44101 accatcgctg atagtacagg cgggtggcga aaccagcgtg ccgtgaaaag taacgtcgtt
    44161 aatcgcggcc tgcgccaggc cagcaaaacc gtaaattaag caccatgcga gtcgtttcat
    44221 tggtagtcca ccaccatggt catggtcgca ttaaactccg atgccatcag ttccacaccg
    44281 ctctgcttca ccggaacggc ttcgaagtct ggctgtgtat tgatgttgaa cggcagccag
    44341 ctactgttac ccactgccac cagcgaatgg tcagcggcat tctgaatacg aatgccaaaa
    44401 ccgctgattc cagtactcag caccgtttcg ccgttgatgg tcgtggtggt ggcctgaagc
    44461 tgcatttgca ggtcgtcgct gacactgttt gagcagttca gggtgtagcc cactggctgg
    44521 cggtagttgg caccatcaat gcttttaata atcacattgc cgaactctac cgcgcttccc
    44581 tttaccgaac agggcggcgg cccatccacc agcacgcgca acgtcaaatt gacgattttg
    44641 ccgcccgcca ttgccgggaa aacggtcacg ccacaggcca gcaatagcgc ggcagatccg
    44701 gtcagaaaga acgctttcat gttgcttctc ccgttatcaa cctttgctgc tttgatcttc
    44761 atccaccttg caggtgttcc cttcacaacg gaagatcagc ggcatgcgcg cgccgtagtc
    44821 gttgatatag gtcagcaccg gggcgctcgc catttttgcc tttaacggtt gtgacgtttt
    44881 cggtggcatc accagcggtt taaacccggc ggcagtcagc cctttcttct gtgtgctggc
    44941 atcgctgatg atgacgtggt acggcgtcgg gttattgacg gtatagtcct ggccactgcg
    45001 cgtgagtgtg accttaaact gccacggtct tctcatgtcg atcttctcca gcgcctttgg
    45061 tcgccagaac agcttgatgc gcgtttgcag ggcaatttgc agggtgttgg ctttcccgga
    45121 ctttggcggg atctcacgca cgttgtagta gaacaggctt tctcggtcgg acggcagcgt
    45181 gtgaatgtcc ggcagcgcct gaatcttcac ctggccgttc atcatagcgt caatacgctg
    45241 caccggcggc agtactgcca gcggcgagat gattttgttg cccttctcgt cttccagcca
    45301 gctttgcgcc aggtaaggca gcttttcgtt gttgttacgc aacgtcacgc tgatcgattt
    45361 atcgctttcg ttaaacacca gacgggtgcg atccggggtg accgatgcat gcgccgtcag
    45421 gctggcggtg gtaagcattg ccatcagtgc tgtgctgacg agcattggac aggataattt
    45481 ataagacatt gatcgttcct taatcagatt attgagttgc tgaaattgac gtcggcgctt
    45541 ccgttggtgt gacttgccgc gtctggtcct gaataacagg cttaatttct ggcgctgaag
    45601 aatcaggggc tgccgttccc ttttgttcac acggcaacaa caggccgctg aacagatcgg
    45661 caggtagcgg cttcggcaat acgatctcgc attgtgcgct gccttcccag aacaccatca
    45721 tatgctcgtc cgcattgacc cccgccagat agacattgcc ttcatcatcc acaatgccaa
    45781 cctgctgctg ctcgtcgttt ttcacttcca cgccaaacgg cgggtagcta ccgtcacgca
    45841 accgcagtac cgccatcgct ttttgcccgc tgatcacttt gaatttacga tagccgattg
    45901 cgccttccgt cagagtcgcc tgcaccaccg actgggtggc ttccgcatct tccggcaagt
    45961 tgtttaaatc gatatacgcc tgattgcgat agtagttgtt aatatcggcg accaccgcct
    46021 tgccaaacat attggtgtac accggcgcgc cgttgctttc gaccggcacg ttggcaatcc
    46081 cgtcggcgtc aatcagcagg cgcgtgccgc ccatgttctg ggtacggtgc agcgccccac
    46141 catgcgcggt cagcgttgcc ccgccctgta aggcgatccc cgccgaacgg tattcccctt
    46201 catggtagtt ggcgctgaga tcaaccttcg ccagcgaacc gtcgtggctc agataaccat
    46261 ccacgctgcc gtgctgctcg ctggtgccaa cgttaacctg gtagtgcgtt gcgtcatcga
    46321 cgcgcttaaa gtaaccgacc tggcagctgt ccgacccgct gccgtaggaa ccgttgtagg
    46381 tcacggtgct gctgtcgctc cagggaatgc tcatactgag gtacatcccc ttatccgcat
    46441 tatcgtcata ttcgtagcga tagccggtca ccgagatgct catgttgcga atgctcccca
    46501 tattaaaata gtgggaaaac atcaggttat agtttgtctg ttccgggcga tcccagtaag
    46561 tacgatggga gtagttgaga tagatcgaga cacccgcagc ggcaaagttc tggttatagg
    46621 tgatcgtata catctctttg tcgttaccgg tccgcgccat gtccgactgg ttcgcgtcca
    46681 gatactcgct catggtcatg aagttctttt cagaaaaacg gtagcctgca aaggtgacgc
    46741 ggctgttgag ttcgtcaaag tctttggcat agctcacgcg aaaggagttg ccgtccagtt
    46801 ttcctttgcc gtatgcgctg tcatgatcca ggttgacgtg cgagtgagtc acatcgaacg
    46861 ccagcgcgcc aaactgtgcg aggtcgcgcc ccacccccat cgccgccgac tgatagtgtt
    46921 tatctgccag cgcaccaccg tagagcgacc agccatctgc caccccccat gacgcttctc
    46981 cgccggagaa aaagccgcct tcggtcttgt ggttccagtc ttccggacgc cccatcatca
    47041 ctttgtaacg cacctgcccc tggcgcgtga ggaatggcat agatgcggtg gtaacgtcat
    47101 attcctgcac ctgaccattc tgttcttcaa cgcggacgtg cagtgtgccg gagacggagt
    47161 cgccgatatc ctgaatgcgg aatggcccgg cgggaacctg ggtttcgtaa agtacccggc
    47221 ccatctggct aatggtgact tttgcactgc tgtgcgccac gccggaaaca tccggtgcat
    47281 agccgcgcag gttgggcggc agcatttggt catcggtgtt gacgctgctg ccgatatagt
    47341 taaagccgtc gaaaatatcg gaattgagat aatcttcccc cagcgacagc ttcgctttta
    47401 aagagggcaa ggctcgccag gcataataac gactccagtc ccagtgttta ctcgttgtgc
    47461 tgttactgct gtcatcgtcg tcatcgttac tgcgggtgtg ctgataatca ctttgccagt
    47521 ctgcacggaa acgccatgcc cccaggttcg ccccaacggt accgttgccg ctgatatcat
    47581 gtgagtcctc gcccccctgc tcctggtggc gagtttgcgc attgaggctg tagtcggcaa
    47641 tcaggccagg aataccgtcg tcccaacgtg agggagggtc ccagtcgctg ctggtatatt
    47701 cgaggtaagc ctgtggcact gtcagcagca acgccgactg gcttaaatca ttctcgactt
    47761 ccatcccgtc taattgacct agtttaaggc attcatcgtt gtgcgtccac tgcaggcttt
    47821 ttgctatccc ttctttcagc cccagcgccg ccactaactc aggtgtcagg caggcatagt
    47881 tttttgttgg atcgttttca gagacgtacc agttaatgtc gtat
//
LOCUS       DADSYM010000011        46844 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2415
            SAMN08039579-rid11723173.denovo.011, whole genome shotgun sequence.
ACCESSION   DADSYM010000011 DADSYM010000000
VERSION     DADSYM010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039579
            Sequence Read Archive: SRR6362533
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 46844)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 46844)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980162.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 03:39:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,484
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 4,347
            CDSs (with protein)               :: 4,347
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 43 of 85
            Pseudo Genes (incomplete)         :: 37 of 85
            Pseudo Genes (internal stop)      :: 19 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..46844
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039579-rid11723173.denovo.011"
                     /strain="LGE2415"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8302 N 95.9628 W"
                     /collection_date="2015-08-24"
     gene            <1..1359
                     /locus_tag="J1Y83_000537"
     CDS             <1..1359
                     /locus_tag="J1Y83_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA7628723.1"
                     /translation="TIENKRYFNNGKIKDGECPHFYEDGKIKQQHSYLNSKLDGPAYE
                     YYPDGKLMQENFYQQSELIGKDTSYYQSGKINSIHNRNSRGQYDGINERYSEEGKLLS
                     KSVYKDGKQISVQTWYENGQKEEEKHFDVQGQLNGLVKQWYKNGNLAKSQNYKHDILD
                     GDSEEWYENGIPESLYPYKNGKTDGVAKSWNKYGKLTYSIEYKNGVENGVYRNWSKNT
                     GKLTKETQYVNGIRQGVEKEFNDRTGKLLTATQYVNNKRNGTEETYDQNGIKYITCYQ
                     NDEELSSLYTPTQIKDNATKGNSSAQFTLGKYEFTCANIDEGIKWLTKSAEQKNTDAI
                     YFLATAYKGNGIPANNEKYITYLQQAAMLGNSNAQAEIGYLYLIGKELPQNLPDAGVW
                     FKKAAAQGNFVAHFYLGRMYQNGDGVEKNMEKARFHLSNAAEGGIKPALKALNELEHQ
                     TK"
     gene            1412..3121
                     /locus_tag="J1Y83_000538"
     CDS             1412..3121
                     /locus_tag="J1Y83_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA7628724.1"
                     /translation="MKVKSTLLLIILSVFYSHQVLSSAPTQPDVYSVVEQKLNSALPL
                     KENTTFRSQAEWFNLQYKLLVSGYPERAYNLLSKLETESKNAKYYLDLTASRVAEEGA
                     PETALTFLSKIGLNKPATLTSFESYINSWIKSKQPDKAMQLLSLDDNALHYYLPTVLV
                     AYQSDPDKAISIYNNVYGDNVIIPYQQATMLLTVAEGYQTKGDIKNAIHYADNALNMF
                     GSRESHYGDEYLKLMNIYATNGNKEKAVVLSQRLQKTITESQSNYLSTLEGILLFYRN
                     NDMQQDYQNSLSNYIVFIDKTFAFSPSTRSELSLINLLNSLNEVELMKKRLALLMTAP
                     EYTCYDSQYCYEDKVSALKTLHSHQDNELMDKYFTTLLKEISNQPLEDWDIIISNTGE
                     KFSEVGLTKYAQHLASFAEEFYIKEQKSHSESEMRGLFSSLAEIYSFGNDTASANRVL
                     HQHVPAFNTDHMIQYYINAKEWNNARELLIKEEMLGMHNLILLENICMQKTAQCMAHI
                     TFTLNKLTTQPAITKKDSVGNEQLYQIGKIYHKLGVKPEPKQQALIQSLYDQASGSTS
                     STQ"
     gene            complement(3181..3672)
                     /gene="yajQ"
                     /locus_tag="J1Y83_000539"
     CDS             complement(3181..3672)
                     /gene="yajQ"
                     /locus_tag="J1Y83_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414960.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide binding protein YajQ"
                     /protein_id="HBA7628725.1"
                     /translation="MPSFDIVSEVDLQEARNAVDNASREVESRFDFRNVEASFELNDA
                     SKTIKVLSESDFQVNQLLDILRAKLLKRGIEGSSLDVPENIVHSGKTWFVEAKLKQGI
                     ESATQKKIVKMIKDSKLKVQAQIQGDEIRVTGKSRDDLQAVMAMVRGGDLGQPFQFKN
                     FRD"
     gene            3840..4751
                     /gene="panE"
                     /locus_tag="J1Y83_000540"
     CDS             3840..4751
                     /gene="panE"
                     /locus_tag="J1Y83_000540"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HBA7628726.1"
                     /translation="MKITVLGCGALGQLWLTALCKQGHDVQGWLRVPQPYCSVNLVET
                     DGSIFNESLTANDPDFLATSDLLLVTLKAWQVSDAVKSLASTLPVTTPILLIHNGMGT
                     IEELQNIQQPLLMGTTTHAARRDGNVIIHVANGITHIGPARQQDGDYSYLADILQTVL
                     PDVAWHNNIRAELWRKLAVNCVINPLTAIWNCPNGELRHHPQEIMQICEEVAAVVERE
                     GHHTSAEDLRDYVMQVIDATAENISSMLQDIRALRHTEIDYINGFLLRRARAHGIAVP
                     ENTRLFEMVKRKESEYERIGTGLPRPW"
     gene            4714..5304
                     /gene="yajL"
                     /locus_tag="J1Y83_000541"
     CDS             4714..5304
                     /gene="yajL"
                     /locus_tag="J1Y83_000541"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein deglycase YajL"
                     /protein_id="HBA7628727.1"
                     /translation="MSASALVCLAPGSEETEAVTTIDLLVRGGIKVTTASVASDGNLA
                     ITCSRGVKLLADAPLVEVADGEYDVIVLPGGIKGAECFRDSTLLVETVKQFHRSGRIV
                     AAICAAPATVLVPHDIFPIGNMTGFPTLKDKIPAEQWQDKRVVWDARVKLLTSQGPGT
                     AIDFGLKIIDLLVGREKAHEVASQLVMAAGIYNYYE"
     gene            complement(5358..6806)
                     /gene="thiI"
                     /locus_tag="J1Y83_000542"
     CDS             complement(5358..6806)
                     /gene="thiI"
                     /locus_tag="J1Y83_000542"
                     /EC_number="2.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014168857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 4-thiouridine(8) synthase ThiI"
                     /protein_id="HBA7628728.1"
                     /translation="MKFIIKLFPEITIKSQSVRLRFIKILTGNIRNVLKHYDEMLAVV
                     RHWDNIEVRAKDENQRLAIRDALTRIPGIHHILEVEDVPFTDMHDIFEKALVQYRDQL
                     EGKTFCVRVKRRGKHDFSSIDVERYVGGGLNQHIESARVKLTNPEVTVHLEVEDDRLL
                     LIKGRYEGIGGFPIGTQEDVLSLISGGFDSGVSSYMLMRRGCRVHYCFFNLGGAAHEI
                     GVRQVAHYLWNRFGSSHRVRFVAINFEPVVGEILEKIDDGQMGVILKRMMVRAASKVA
                     ERYGVQALVTGEALGQVSSQTLTNLRLIDNVSDTLILRPLISYDKEHIINLARQIGTE
                     DFARTMPEYCGVISKSPTVKAVKSKIEAEEEKFDFSILDKVVEEANNVDIREIAQQTE
                     QEVVEVETVNGFGPNDVILDIRSIDEQEDKPLKVEGIDVVSLPFYKLSTKFGDLDQSK
                     TWLLWCERGVMSRLQALYLREQGFNNVKVYRP"
     gene            7012..7254
                     /gene="xseB"
                     /locus_tag="J1Y83_000543"
     CDS             7012..7254
                     /gene="xseB"
                     /locus_tag="J1Y83_000543"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015571292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HBA7628729.1"
                     /translation="MPKKNEAPASFEKALSELEQIVTRLESGDLPLEEALNEFERGVQ
                     LARQGQAKLQQAEQRVQILLSDNEDASLTPFTPDNE"
     gene            7254..8153
                     /gene="ispA"
                     /locus_tag="J1Y83_000544"
     CDS             7254..8153
                     /gene="ispA"
                     /locus_tag="J1Y83_000544"
                     /EC_number="2.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2E,6E)-farnesyl diphosphate synthase"
                     /protein_id="HBA7628730.1"
                     /translation="MDFPQQLEACVKQANQALSRFIAPLPFQNTPVVETMQYGALLGG
                     KRLRPFLVYATGHMFGVSTNTLDAPAAAVECIHAYSLIHDDLPAMDDDDLRRGLPTCH
                     VKFGEANAILAGDALQTLAFSILSDADMPEVSDRDRISMISELASASGIAGMCGGQAL
                     DLDAEGKHVPLDALERIHRHKTGALIRAAVRLGALSAGDKGRRALPVLDKYAESIGLA
                     FQVQDDILDVVGDTATLGKRQGADQQLGKSTYPALLGLEQARKKARDLIDDARQSLKQ
                     LTEQSLDTSALEALADYIIQRNK"
     gene            8178..10040
                     /gene="dxs"
                     /locus_tag="J1Y83_000545"
     CDS             8178..10040
                     /gene="dxs"
                     /locus_tag="J1Y83_000545"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HBA7628731.1"
                     /translation="MSFDIAKYPTLALVDSTQELRLLPKESLPKLCDELRRYLLDSVS
                     RSSGHFASGLGTVELTVALHYVYNTPFDQLIWDVGHQAYPHKILTGRRDKIGTIRQKG
                     GLHPFPWRGESEYDVLSVGHSSTSISAGIGIAVAAEKEGKNRRTVCVIGDGAITAGMA
                     FEAMNHAGDIRPDMLVVLNDNEMSISENVGALNNHLAQLLSGKLYSSLREGGKKVFSG
                     VPPIKELLKRTEEHIKGMVVPGTLFEELGFNYIGPVDGHDVLGLITTLKNMRDLKGPQ
                     FLHIMTKKGRGYEPAEKDPITFHAVPKFDPSSGCLPKSSGGLPSYSKIFGDWLCETAA
                     KDNKLMAITPAMREGSGMVEFSRKFPDRYFDVAIAEQHAVTFAAGLAIGGYKPIVAIY
                     STFLQRAYDQVLHDVAIQKLPVLFAIDRAGIVGADGQTHQGAFDLSYLRCIPEMVIMT
                     PSDENECRQMLYTGYHYNDGPSAVRYPRGNAVGVELTPLEKLPIGKGIVKRRGEKLAI
                     LNFGTLMPEAAKVAESLNATLVDMRFVKPLDEALILEMAASHEALVTVEENAIMGGAG
                     SGVNEVLMAHRKPVPVLNIGLPDFFIPQGTQEEMRAELGLDATGMEAKIKAWLA"
     gene            10095..11069
                     /gene="yajO"
                     /locus_tag="J1Y83_000546"
     CDS             10095..11069
                     /gene="yajO"
                     /locus_tag="J1Y83_000546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414953.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxyxylulose-5-phosphate synthase YajO"
                     /protein_id="HBA7628732.1"
                     /translation="MQYNPLGKTDLRVSRLCLGCMTFGEPDRGNHAWTLPEESSRPII
                     KRALEGGINFFDTANSYSDGSSEEIVGRALRDFARREDVVVATKVFHRVGDLPEGLSR
                     AQILRSIDDSLLRLGMDYVDILQIHRWDYNTPIEETLEALNDVVKAGKARYIGASSMH
                     ASQFAQALELQKQHGWAQFVSMQDHYNLIYREEEREMLPLCYQEGVAVIPWSPLARGR
                     LTRPWGETTARLVSDEVGKNLYKESDENDAQIAERLTGVSEELGATRAQVALAWLLSK
                     PGIAAPIIGTSREEQLDELLNAVDITLKPEQIAELETPYKPHPVVGFK"
     gene            complement(11123..11641)
                     /gene="pgpA"
                     /locus_tag="J1Y83_000547"
     CDS             complement(11123..11641)
                     /gene="pgpA"
                     /locus_tag="J1Y83_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HBA7628733.1"
                     /translation="MTILPRHKDVAKSRLKMSNPWHLLAVGFGSGLSPIVPGTMGSLA
                     AIPFWYLMTFLPWQLYSLVVMLGICIGVYLCHQTAKDMGVHDHGSIVWDEFIGMWITL
                     MALPTNDWQWVAAGFVIFRILDMWKPWPIRWFDRNVHGGMGIMIDDIVAGVISAGILY
                     FIGHHWPLGILS"
     gene            complement(11619..12596)
                     /gene="thiL"
                     /locus_tag="J1Y83_000548"
     CDS             complement(11619..12596)
                     /gene="thiL"
                     /locus_tag="J1Y83_000548"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HBA7628734.1"
                     /translation="MACGEFSLIARYFDRVRSSRLDVELGIGDDCALLNIPEKQTLAI
                     STDTLVAGNHFLPDIDPADLAYKALAVNLSDLAAMGADPAWLTLALTLPDVDEAWLES
                     FSDSLFDLLNYYDMQLIGGDTTRGPLSMTLGIHGFVPMGRALTRSGAKPGDWIYVTGT
                     PGDSAAGLAILQNRLQVADAKDADYLIKRHLRPSPRILQGQALRDLANSAIDLSDGLI
                     SDLGHIVKASDCGARIDLALLPFSDALSRHVEPEQALRWALSGGEDYELCFTVPELNR
                     GALDVALGHLGVPFTCIGQMTADIEGLCFIRDGEPVTLDWKGYDHFATP"
     gene            complement(12674..13093)
                     /gene="nusB"
                     /locus_tag="J1Y83_000549"
     CDS             complement(12674..13093)
                     /gene="nusB"
                     /locus_tag="J1Y83_000549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HBA7628735.1"
                     /translation="MKPAARRRARECAVQALYSWQLSQNDIADVEYQFLAEQDVKDVD
                     VLYFRELLAGVATNTAYLDGLMKPYLSRLLEELGQVEKAVLRIALYELSKRSDVPYKV
                     AINEAIELAKSFGAEDSHKFVNGVLDKAAPVIRPNKK"
     gene            complement(13113..13583)
                     /gene="ribE"
                     /locus_tag="J1Y83_000550"
     CDS             complement(13113..13583)
                     /gene="ribE"
                     /locus_tag="J1Y83_000550"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HBA7628736.1"
                     /translation="MNIIEANVATPDARVAITIARFNNFINDSLLEGAIDALKRIGQV
                     KDENITVVWVPGAYELPLAAGALAKTGKYDAVIALGTVIRGGTAHFEYVAGGASNGLA
                     HVAQDSEIPVAFGVLTTESIEQAIERAGTKAGNKGAEAALTALEMINVLKAIKA"
     gene            complement(13672..14775)
                     /gene="ribD"
                     /locus_tag="J1Y83_000551"
     CDS             complement(13672..14775)
                     /gene="ribD"
                     /locus_tag="J1Y83_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HBA7628737.1"
                     /translation="MQDEYYMARALKLAQRGRFTTHPNPNVGCVIVKDGEIVGEGYHQ
                     RAGEPHAEVHALRMAGEKAKGATAYVTLEPCSHHGRTPPCCDALIAAGVACVVAAMQD
                     PNPQVAGRGLYRLQQAGIDVSHGLMMSEAEQLNKGFLKRMRTGFPYIQLKLGASLDGR
                     TAMASGESQWITSPQARRDVQRLRAQSHAILTSSATVLADDPALTVRWSELDEQTQAL
                     YPQQNLRQPIRIVIDSQNRVTPEHRIVQQPGETWFARTQDDSREWPETVRTLLIPEHK
                     GHLDLVVLMMQLGKQQINSIWVEAGPTLAGALLQAGLVDELIVYIAPKLLGNDARGLC
                     TLPGLEKLADAPQFKFKEIRHVGPDVCLHLVGA"
     gene            complement(14779..15228)
                     /gene="nrdR"
                     /locus_tag="J1Y83_000552"
     CDS             complement(14779..15228)
                     /gene="nrdR"
                     /locus_tag="J1Y83_000552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HBA7628738.1"
                     /translation="MHCPFCFAVDTKVIDSRLVGEGSSVRRRRQCLVCNERFTTFEVA
                     ELVMPRVVKSNDVREPFNEEKLRSGMLRALEKRPVSSDDVEMAINHIKSQLRATGERE
                     VPSKMIGNLVMEQLKKLDKVAYIRFASVYRSFEDIKEFGEEIARLED"
     gene            15379..15918
                     /locus_tag="J1Y83_000553"
     CDS             15379..15918
                     /locus_tag="J1Y83_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414946.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3251 domain-containing protein"
                     /protein_id="HBA7628739.1"
                     /translation="MNTNVFRLLLLGSLFSLSACVQQSEVRQIKHSVSTLNQEMTQLN
                     KETVKITQQNKLNAKSSSGVYLLPGAKTPARLESQIGTLRMSLVNITPDTDGTTLTLR
                     IQGESNDPLPAFSGTVEYGQIQGTIDNFQEINVQNQLINAPASVLAPSDVDIPLQLKG
                     MSVDQLGFVRIHDIQPVMQ"
     gene            16217..17101
                     /locus_tag="J1Y83_000554"
     CDS             16217..17101
                     /locus_tag="J1Y83_000554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-specific channel-forming protein Tsx"
                     /protein_id="HBA7628740.1"
                     /translation="MKKTLLAAGAVLALSSSFTVNAAENDKPQYLSDWWHQSVNVVGS
                     YHTRFGPQLRNDTYLEYEAFAKKDWFDFYGYADAPVFFGGNSDAKGIWNHGSPLFMEI
                     EPRFSIDKLTNTDLSFGPFKEWYFANNYIYDMGRNKDGRQSTWYMGLGTDIDTGLPMS
                     LSMNVYAKYQWQNYGAANENEWDGYRFKVKYFVPITDLWGGQLSYIGFTNFDWGSDLG
                     DDSGYANNGIKTRTNNSIASSHILALNYDHWHYSVVARYWHNGGQWNDDAELNFGNGN
                     FNVRSTGWGGYLVVGYNF"
     gene            complement(17139..17486)
                     /locus_tag="J1Y83_000555"
     CDS             complement(17139..17486)
                     /locus_tag="J1Y83_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH nuclease family protein"
                     /protein_id="HBA7628741.1"
                     /translation="MAIIPKNYARLESGYREKALKIYPWVCGRCSREFVYSNLRELTV
                     HHIDHDHTNNPEDGSNWELLCLYCHDHEHSKYTEADQYGTTVIAGEDAQKDVGEAKYN
                     PFADLKAMMNKKK"
     gene            complement(17616..18587)
                     /gene="secF"
                     /locus_tag="J1Y83_000556"
     CDS             complement(17616..18587)
                     /gene="secF"
                     /locus_tag="J1Y83_000556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HBA7628742.1"
                     /translation="MAQEYTVEQLNHGRKVYDFMRWDYWAFGISGLLLIAAIVIMGVR
                     GFNWGLDFTGGTVIEITLEKPAEIDVMRDALQKAGFEEPMLQNFGSSHDIMVRMPPAE
                     GETGGQVLGSQVLKVINESTNQNAAVKRIEFVGPSVGADLAQTGAMALMAALLSILVY
                     VGFRFEWRLAAGVVIALAHDVIITLGILSLFHIEIDLTIVASLMSVIGYSLNDSIVVS
                     DRIRENFRKIRRGTPYEIFNVSLTQTLHRTLITSGTTLMVILMLYLFGGPVLEGFSLT
                     MLIGVSIGTASSIYVASALALKLGMKREHMLQQKVEKEGADQPSILP"
     gene            complement(18598..20445)
                     /gene="secD"
                     /locus_tag="J1Y83_000557"
     CDS             complement(18598..20445)
                     /gene="secD"
                     /locus_tag="J1Y83_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HBA7628743.1"
                     /translation="MLNRYPLWKYVMLIVVIVIGLLYALPNLFGEDPAVQITGARGVA
                     ASEQTLIQVQKTLQEEKITAKSVALEEGAILARFDSTDTQLRAREALMGVMGDKYVVA
                     LNLAPATPRWLAAIHAEPMKLGLDLRGGVHFLMEVDMDTALGKLQEQNIDSLRSDLRE
                     KGIPYTTVRKENNYGLSITFRDAKARDEAIAYLSKRHPDLVISSQGSNQLRAVMSDAR
                     LSEAREYAVQQNINILRNRVNQLGVAEPVVQRQGADRIVVELPGIQDTARAKEILGAT
                     ATLEFRLVNTNVDQAAAASGRVPGDSEVKQTREGQPVVLYKRVILTGDHITDSTSSQD
                     EYNQPQVNISLDSAGGNIMSNFTKDNIGKPMATLFVEYKDSGKKDANGRAVLVKQEEV
                     INIANIQSRLGNSFRITGINNPSEARQLSLLLRAGALIAPIQIVEERTIGPTLGMQNI
                     EQGLEACLAGLLVSILFMIIFYKKFGLIATSALIANLILIVGIMSLLPGATLSMPGIA
                     GIVLTLAVAVDANVLINERIKEELSNGRTVQQAIDEGYRGAFSSIFDANITTLIKVII
                     LYAVGTGAIKGFAITTGIGVATSMFTAIVGTRAIVNLLYGGKRVKKLSI"
     gene            complement(20473..20805)
                     /gene="yajC"
                     /locus_tag="J1Y83_000558"
     CDS             complement(20473..20805)
                     /gene="yajC"
                     /locus_tag="J1Y83_000558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000007628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HBA7628744.1"
                     /translation="MSFFISDAVAATGAPAQGSPMSLILMLVVFGLIFYFMILRPQQK
                     RTKEHKKLMDSIAKGDEVLTNGGLVGRVTKVAENGYIAIALNDTTEVVIKRDFVAAVL
                     PKGTMKAL"
     gene            complement(20828..21955)
                     /gene="tgt"
                     /locus_tag="J1Y83_000559"
     CDS             complement(20828..21955)
                     /gene="tgt"
                     /locus_tag="J1Y83_000559"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HBA7628745.1"
                     /translation="MKFELDTTDGRARRGRLVFDRGVVETPCFMPVGTYGTVKGMTPE
                     EVEATGAQIILGNTFHLWLRPGQEIMKLHGDLHDFMQWKGPILTDSGGFQVFSLGDIR
                     KITEQGVHFRNPINGDPIFLDPEKSMEIQYDLGSDIVMIFDECTPYPADWDYAKRSME
                     MSLRWAKRSRERFDSLGNKNALFGIIQGSVYEDLRDISVKGLVDIGFDGYAVGGLAVG
                     EPKADMHRILEHVCPQIPADKPRYLMGVGKPEDLVEGVRRGIDMFDCVMPTRNARNGH
                     LFVTDGVVKIRNAKYKSDTGPLDPECDCYTCRNYSRAYLHHLDRCNEILGARLNTIHN
                     LRYYQRLMAGLRKAIEEGKLESFVTDFYQRQGREVPPLNVD"
     gene            complement(22010..23080)
                     /gene="queA"
                     /locus_tag="J1Y83_000560"
     CDS             complement(22010..23080)
                     /gene="queA"
                     /locus_tag="J1Y83_000560"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HBA7628746.1"
                     /translation="MRVTDFSFELPESLIAHYPMPERSSCRLLSLDGPTGALTHGTFT
                     DLLDKLNPGDLLVFNNTRVIPARLFGRKASGGKIEVLVERMLDDKRILAHIRASKAPK
                     PGAELLLGDDESINATMTARHGALFEVEFNDQRSVLDILNSIGHMPLPPYIDRPDEDA
                     DRELYQTVYSEKPGAVAAPTAGLHFDEPLLEKLRAKGVEMAFVTLHVGAGTFQPVRVD
                     TIEDHIMHSEYAEVPQDVVDAVLAAKARGNRVIAVGTTSVRSLESAAQAAKNDLIEPF
                     FDDTQIFIYPGFQYKVVDALVTNFHLPESTLIMLVSAFAGYQHTMNAYKAAVEEKYRF
                     FSYGDAMFITYNPQAINERVGE"
     gene            23173..23754
                     /gene="acpH"
                     /locus_tag="J1Y83_000561"
     CDS             23173..23754
                     /gene="acpH"
                     /locus_tag="J1Y83_000561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP phosphodiesterase"
                     /protein_id="HBA7628747.1"
                     /translation="MNFLAHLHLAHLAESSLSGNLLADFVRGNPEESFPPDVVAGIHM
                     HRRIDVLTDNLPEVREAREWFRNETRRVAPITLDVMWDHFLSRHWSQLSPDFPLQEFT
                     CYAREQVMTILPDSPPRFINLNNYLWSEQWLVRYRDMDFIQSVLNGMASRRPRLDALR
                     DSWYDLDAHYDALETRFWQFYPRMMEQASRKAL"
     gene            complement(23759..25576)
                     /gene="malZ"
                     /locus_tag="J1Y83_000562"
     CDS             complement(23759..25576)
                     /gene="malZ"
                     /locus_tag="J1Y83_000562"
                     /EC_number="3.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308480.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrin glucosidase"
                     /protein_id="HBA7628748.1"
                     /translation="MMLNAWHLPVPPFVKQNKDQLLITLWLTGEDPPQRIMLRTEHDN
                     EETSVAMHKQRSQPQPGVTAWRAAIDLSSGQPRRRYSFKLLWHDRQRWFTPQGFSRMP
                     PARLEQFAVDVPDIGPQWAADQIFYQIFPDRFARSLPREAEQDHVYYHHAAGQEIILR
                     DWDEPVTAQAGGSTFYGGDLDGISEKLPYLKKLGVTALYLNPVFKAPSVHKYDTEDYR
                     HVDPQFGGDGALLRLRHNTQQLGMRLVLDGVFNHSGDSHAWFDRHNRGTGGACHNPES
                     PWRDWYSFSDDGTALDWLGYASLPKLDYQSESLVNEIYRGEDSIVRHWLKAPWNMDGW
                     RLDVVHMLGEAGGARNNLQHVAGITEAAKETQPDAYIVGEHFGDARQWLQADVEDAAM
                     NYRGFTFPLWGFLANTDISYDPQQIDAQTCMAWMDNYRAGLSHQQQLRMFNQLDSHDT
                     ARFKTLLGRDVARLPLAVVWLFTWPGVPCIYYGDEVGLDGKNDPFCRKPFPWQVEKQD
                     SALFALYQRMIALRKKGQALRRGGCQVLYAEDNVVVFVRVLNQQRVLVAINRGEACEV
                     VLPASPLLNVAQWQRKEGHGQLTDGILALPAISATVWMN"
     gene            complement(25732..27105)
                     /gene="proY"
                     /locus_tag="J1Y83_000563"
     CDS             complement(25732..27105)
                     /gene="proY"
                     /locus_tag="J1Y83_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016243349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline-specific permease ProY"
                     /protein_id="HBA7628749.1"
                     /translation="MESKNKLKRGLSTRHIRFMALGSAIGTGLFYGSADAIKMAGPSV
                     LLAYIIGGIAAYIIMRALGEMSVHNPAASSFSRYAQENLGPLAGYITGWTYCFEILIV
                     AIADVTAFGIYMGVWFPTVPHWIWVLSVVLIICAVNLMSVKVFGELEFWFSFFKVATI
                     IIMIVAGFGIIIWGIGNGGQPTGIHNLWSNGGFFSNGWLGMVMSLQMVMFAYGGIEII
                     GITAGEAKDPEKSIPRAINSVPMRILVFYVGTLFVIMSIYPWNQVGTAGSPFVLTFQH
                     MGITFAASILNFVVLTASLSAINSDVFGVGRMLHGMAEQGSAPKIFSKTSRRGIPWVT
                     VLVMTTALLFAVYLNYIMPENVFLVIASLATFATVWVWIMILLSQIAFRRRLPPEEVK
                     ALKFKVPGGVATTIGGLIFLLFIIGLIGYHPDTRISLYVGFAWIVVLLIGWMFKRRHD
                     RQLAENH"
     gene            complement(27181..28500)
                     /gene="brnQ"
                     /locus_tag="J1Y83_000564"
     CDS             complement(27181..28500)
                     /gene="brnQ"
                     /locus_tag="J1Y83_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transporter carrier
                     protein BrnQ"
                     /protein_id="HBA7628750.1"
                     /translation="MTHQLRSRDIIALGFMTFALFVGAGNIIFPPMVGLQAGEHVWTA
                     AFGFLITAVGLPVLTVVALAKVGGGVDSLSTPIGKVAGVLLATVCYLAVGPLFATPRT
                     ATVSFEVGIAPLTGDSALPLFIYSLVYFAIVILVSLYPGKLLDTVGNFLAPLKIIALV
                     ILSVAAIIWPAGSISTATEAYQNAAFSNGFVNGYLTMDTLGAMVFGIVIVNAARSRGV
                     TEARLLTRYTVWAGLMAGVGLTLLYLALFRLGSDSASLVDQSANGAAILHAYVQHTFG
                     GGGSFLLAALIFIACLVTAVGLTCACAEFFAQYVPLSYRTLVFILGGFSMVVSNLGLS
                     QLIQISVPVLTAIYPPCIALVVLSFTRSWWHNSSRVIAPPMFISLLFGILDGIKASAF
                     SDILPSWAQRLPLAEQGLAWLMPTVVMVVLAIIWDRAAGRQVTSSAH"
     gene            complement(28907..30202)
                     /gene="phoR"
                     /locus_tag="J1Y83_000565"
     CDS             complement(28907..30202)
                     /gene="phoR"
                     /locus_tag="J1Y83_000565"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate regulon sensor histidine kinase PhoR"
                     /protein_id="HBA7628751.1"
                     /translation="MLERLSWKRLVLELLLCCLPAFILGAFFGYLPWFLLASVTGLLI
                     WHFWNLLRLSWWLWVDRSMTPPPGRGSWEPLLYGLHQMQLRNKKRRRELGNLIKRFRS
                     GAESLPDAVVLTTEEGGIFWCNGLAQQILGLRWPEDNGQNILNLLRYPEFTQYLKTRD
                     FSRPLNLVLNTGRHLEIRVMPYTHKQLLMVARDVTQMHQLEGARRNFFANVSHELRTP
                     LTVLQGYLEMMDEQPLEGAVREKALHTMREQTQRMEGLVKQLLTLSKIEAAPTHLLNE
                     KVDVPMMLRVVEREAQTLSQKKQTFTFEIDNGLKVSGNEDQLRSAISNLVYNAVNHTP
                     EGTHITVRWQRVPHGAEFSVEDNGPGIAPEHIPRLTERFYRVDKARSRQTGGSGLGLA
                     IVKHAVNHHESRLNIESTVGKGTRFSFVIPERLIAKNSD"
     gene            complement(30260..30949)
                     /gene="phoB"
                     /locus_tag="J1Y83_000566"
     CDS             complement(30260..30949)
                     /gene="phoB"
                     /locus_tag="J1Y83_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000113934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate response regulator transcription
                     factor PhoB"
                     /protein_id="HBA7628752.1"
                     /translation="MARRILVVEDEAPIREMVCFVLEQNGFQPVEAEDYDSAVNQLNE
                     PWPDLILLDWMLPGGSGIQFIKHLKRESMTRDIPVVMLTARGEEEDRVRGLETGADDY
                     ITKPFSPKELVARIKAVMRRISPMAVEEVIEMQGLSLDPTSHRVMAGEEPLEMGPTEF
                     KLLHFFMTHPERVYSREQLLNHVWGTNVYVEDRTVDVHIRRLRKALEPGGHDRMVQTV
                     RGTGYRFSTRF"
     gene            31139..32341
                     /gene="sbcD"
                     /locus_tag="J1Y83_000567"
     CDS             31139..32341
                     /gene="sbcD"
                     /locus_tag="J1Y83_000567"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease subunit SbcD"
                     /protein_id="HBA7628753.1"
                     /translation="MRILHTSDWHLGQNFYSKSREAEHQAFLDWLLETAQAHQVDAII
                     VAGDVFDTGSPPSYARTLYNRFVVNLQQTGCHLVVLAGNHDSVATLNESRDIMAFLNT
                     TVVASAGHTPQILPRRDGTPGAVLCPIPFLRPRDIITSQAGLNGIEKQQHLLAAITDY
                     YQQHYADACKLRGDQPLPIIATGHLTTVGASKSDAVRDIYIGTLDAFPAQNFPPADYI
                     ALGHIHRAQIIGGMEHVRYCGSPIPLSFDECGKSKYVHLVTFSNGKLESVENLNVPVT
                     QPMAVLKGDLASITAQLEQWRDISQEPPVWLDIEITTDEYLHDIQRKIQALTESLPVE
                     VLLVRRSREQRERVLASQQRETLSELSVEEVFNRRLALEELDESQQQRLQHLFATTLH
                     SLAGEHEA"
     gene            32338..35481
                     /gene="sbcC"
                     /locus_tag="J1Y83_000568"
     CDS             32338..35481
                     /gene="sbcC"
                     /locus_tag="J1Y83_000568"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease subunit SbcC"
                     /protein_id="HBA7628754.1"
                     /translation="MKILSLRLKNLNSLKGEWKIDFTREPFASNGLFAITGPTGAGKT
                     TLLDAICLALYHETPRLSNVSQSQNDLMTHDTAECLAEVEFEVKGEAYRAFWSQNRAR
                     NQPDGNLQVPRVELARCADGKILADKVKDKLELTATLTGLDYGRFTRSMLLSQGQFAA
                     FLNAKPKERAELLEELTGTEIYGQISAMVFEQHKSARTELEKLQAQASGVALLTPEQV
                     QSLTASLQVLTDEEKQLITAQQQEQQSLNWLTRLDELQQEGSRRQQALQQALAEEEKA
                     QPQLAALSLAQPARNLRPHWERIAEYSTALAHTRQQIEEVNTRLQSTMALRASIRHHA
                     AKQSAELQQQQQSLNAWLQEHDRFRQWNNELAGWRAQFSQQASDREHLRQWQQQLTHA
                     EQKLNALAAITLTLTADEVASALAQHAEQRPLRQRLVALHGQIVPQQKRLAQLQVAIQ
                     NVTLEQTQRNAALNEMRHRYKEKTQQLADVKTICEQETRIKTLEAQRAQLQAGQPCPL
                     CGSTSHPAVEAYQALEPGVNQARLLTLEKEVKKLGEEGATLRGQLDALTKQLQRDENE
                     AQSLRQDEQALTQQWQAVTASLNITLQPQDDIQPWLDAQDEHERQLRLLSQRHELQGQ
                     IAAHNQQIIQYQQQIEQRQQQLLTALAGYALTLPQEDEEESWLATRQQEAQSWQHRQN
                     ELTALQNRIQQLTPILETLPQSDELPHSEETVVLENWRQVHEQCLALHSQQQTLQQQD
                     VLAAQSLQKAQAQFDTALQASVFDDQQAFLAALMDEQTLTQLEQLKQNLENQRRQAQT
                     LVTQTAETLTQHQQHRPGGLSLTVTVEQIQQELAQTHQKLRENTTSQGEIRQQLKQDA
                     DNRQQQQTLMQQIAQMTQQVEDWGYLNSLIGSKEGDKFRKFAQGLTLDNLVHLANQQL
                     TRLHGRYLLQRKASEALEVEVVDTWQADAVRDTRTLSGGESFLISLALALALSDLVSH
                     KTRIDSLFLDEGFGTLDSETLDTALDALDALNASGKTIGVISHVEAMKERIPVQIKVK
                     KINGLGYSKLESAFAMK"
     gene            35607..36791
                     /gene="araJ"
                     /locus_tag="J1Y83_000569"
     CDS             35607..36791
                     /gene="araJ"
                     /locus_tag="J1Y83_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414930.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter AraJ"
                     /protein_id="HBA7628755.1"
                     /translation="MKKVILSLALGTFGLGMAEFGIMGVLTELAHNVGISIPAAGHMI
                     SYYALGVVVGAPIIALFSSRYSLKHILLFLVALCVIGNAMFTLSSSYLMLAIGRLVSG
                     FPHGAFFGVGAIVLSKIIKPGKVTAAVAGMVSGMTVANLLGIPLGTYLSQEFSWRYTF
                     LLIAVFNIAVMASVYFWVPDIRDEAKGKLREQFHFLRSPAPWLIFAATMFGNAGVFAW
                     FSYVKPYMMFISGFSETAMTFIMMLVGLGMVLGNMLSGRISGRYSPLRIAAVTDFIIV
                     LALLMLFFFGGMKTTSLIFAFICCAGLFALSAPLQILLLQNAKGGELLGAAGGQIAFN
                     LGSAVGAYCGGMMLTLGLAYNYVALPAALLSFAAMSSLLLYGRYKRQQAADSSVLAKP
                     LG"
     gene            complement(37059..37967)
                     /gene="mak"
                     /locus_tag="J1Y83_000570"
     CDS             complement(37059..37967)
                     /gene="mak"
                     /locus_tag="J1Y83_000570"
                     /EC_number="2.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructokinase"
                     /protein_id="HBA7628756.1"
                     /translation="MRIGIDLGGTKTEVIALGDAGEQLYRHRLPTPRDDYRQTIETIA
                     TLVDMAEQATGQRGTVGMGIPGSISPYTGVVKNANSTWLNGQPFDKDLSARLQREVRL
                     ANDANCLAVSEAVDGAAAGAQTVFAVIIGTGCGAGVAFNGRAHIGGNGTAGEWGHNPL
                     PWMDEDELRYREEVPCYCGKQGCIETFISGTGFATDYRRLSGHALKGSEIIRLVEESD
                     PVAELALRRYELRLAKSLAHVVNILDPDVIVLGGGMSNVDRLYQTVPQLIKQFVFGGE
                     CETPVRKAKHGDSSGVRGAAWLWPQE"
     gene            38092..39003
                     /gene="rdgC"
                     /locus_tag="J1Y83_000571"
     CDS             38092..39003
                     /gene="rdgC"
                     /locus_tag="J1Y83_000571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-associated protein RdgC"
                     /protein_id="HBA7628757.1"
                     /translation="MLWFKNLMVYRLSREISLRAEEMEKQLASMAFTPCGSQDMAKMG
                     WVPPMGSHSDALTHVANGQIVICARKEEKILPSPVIKQALEAKIAKLEAEQARKLKKT
                     EKDSLKDEVLHSLLPRAFSRFSQTMMWIDTVNGLIMVDCASAKKAEDTLALLRKSLGS
                     LPVVPLSMENPIELTLTEWVRSGSTAQGFQLLDEAELKSLLEDGGVIRAKKQDLTSEE
                     ITNHIEAGKVVTKLALDWQQRIQFVMCDDGSLKRLKFCDELRDQNEDIDREDFAQRFD
                     ADFILMTGELAALIQNLIEGLGGEAQR"
     gene            complement(39084..39164)
                     /locus_tag="J1Y83_000572"
     CDS             complement(39084..39164)
                     /locus_tag="J1Y83_000572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA7628758.1"
                     /translation="MTQRPWSKLQHKTHNIAALRIIARCS"
     gene            complement(39161..39502)
                     /locus_tag="J1Y83_000573"
                     /pseudo
     CDS             complement(39161..39502)
                     /locus_tag="J1Y83_000573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308469.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2773 domain-containing protein"
     gene            complement(39650..39934)
                     /gene="ppnP"
                     /locus_tag="J1Y83_000574"
     CDS             complement(39650..39934)
                     /gene="ppnP"
                     /locus_tag="J1Y83_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine/purine nucleoside phosphorylase"
                     /protein_id="HBA7628759.1"
                     /translation="MLQSNEYFSGKVKSIGFSSSSTGRASVGVMVEGEYTFSTAEPEE
                     MTVISGALNVLLPDATDWQVYEAGSVFNVPGHSEFHLQVADPTSYLCRYL"
     gene            complement(40006..40683)
                     /locus_tag="J1Y83_000575"
     CDS             complement(40006..40683)
                     /locus_tag="J1Y83_000575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AroM family protein"
                     /protein_id="HBA7628760.1"
                     /translation="MSASLAILTIGIVPMQEVLPLLTEYIDEDNISHHSLLGKLSREE
                     VMAEYAPEAGEDTILTLLNDNHLAHVSRRKVERDLQGVVEVLDNQGYDVIILMSTANI
                     SSMTARNTIFLEPSRILPPLVSSIVEDHQVGVIVPVEELLTVQAQKWQILQKPPVFSL
                     GNPIHDSEQKIIDAGKELLAKGADVIMLDCLGFNQRHRDLLQKQLDVPVLLSNVLIAR
                     LAAELLM"
     gene            complement(40941..41132)
                     /gene="yaiA"
                     /locus_tag="J1Y83_000576"
     CDS             complement(40941..41132)
                     /gene="yaiA"
                     /locus_tag="J1Y83_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YaiA"
                     /protein_id="HBA7628761.1"
                     /translation="MPTKPPYPREAYIVTIEKGKPGQTVTWYQLRADHPKPDSLISEH
                     PTAQEAMDAKKRYEDPDKE"
     gene            complement(41182..41706)
                     /gene="aroL"
                     /locus_tag="J1Y83_000577"
     CDS             complement(41182..41706)
                     /gene="aroL"
                     /locus_tag="J1Y83_000577"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase AroL"
                     /protein_id="HBA7628762.1"
                     /translation="MTQPLFLIGPRGCGKTTVGMALADSLNRRFVDTDLWLQSQLNMT
                     VAEIVEREEWAGFRARETAALEAVTAPSTVIATGGGIILTKFNRHFMQNNGIVVYLCA
                     PVSVLVNRLQAAPEEDLRPTLTGKPLSEEVQEVLEERDALYREVAHIIIDATNEPSQV
                     ISEIRSALAQTINC"
     gene            complement(41889..42347)
                     /locus_tag="J1Y83_000578"
     CDS             complement(41889..42347)
                     /locus_tag="J1Y83_000578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HBA7628763.1"
                     /translation="MTIWVDADACPNVIKDILYRAAERMQMPLVLVANQSLRVPPSRF
                     IRTLRVAAGFDVADNEIVRQCEAGDLVITADIPLAAEAIEKGAAALNPRGERYTPATI
                     RERLTMRDFMDTLRASGIQTGGPDSLSQRDRQAFAAELEKWWLEVQRSRG"
     gene            42467..43276
                     /gene="proC"
                     /locus_tag="J1Y83_000579"
     CDS             42467..43276
                     /gene="proC"
                     /locus_tag="J1Y83_000579"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HBA7628764.1"
                     /translation="MEKKIGFIGCGNMGKAILGGLIASGQVLPGQIWVYTPSPDKVTA
                     LHDQFGINAAESAQEVAQIADIIFAAVKPGIMIKVLSEITSSLNKDSLVVSIAAGITL
                     DQLARALGHDRKIIRAMPNTPALVNAGMTSVTPNALVTPEDTADVLNIFRCFGEAEVI
                     AEPMIHPVVGVSGSSPAYVFMFIEAMADAAVLGGMPRAQAYKFAAQAVMGSAKMVLET
                     GEHPGTLKDMVCSPGGTTIEAVRVLEEKGFRAAVIEAMTKCMEKSEKLSKS"
     gene            complement(43293..44408)
                     /gene="adrA"
                     /locus_tag="J1Y83_000580"
                     /gene_synonym="dgcC"
     CDS             complement(43293..44408)
                     /gene="adrA"
                     /locus_tag="J1Y83_000580"
                     /gene_synonym="dgcC"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase AdrA"
                     /protein_id="HBA7628765.1"
                     /translation="MFPKIMNDENFFKKAAAHGEEPPLTPQNEHQRSGLRFARRVRLP
                     RAVGLAGMFLPIASTLVSHPSPGWWWLLLVGWAFVWPHLAWQIASRAVDPLSREIYNL
                     KTDAVLAGMWVGVMGVNALPSTAMLMIMCLNLMGAGGPRLFVAGLVLMVVSCLVTLEL
                     TGITVSFNSAPLEWWLSLPIIVIYPLLFGWVSYQTATKLAEHKRRLQVMSTRDGMTGV
                     YNRRHWETMLRNEFDNCRRHNRDATLLIIDIDHFKSINDTWGHDVGDEAIVALTRQLQ
                     ITLRGSDVIGRFGGDEFAVIMSGTPAESAITAMLRVHEGLNTLRLPNTPQVTLRISVG
                     VAPLNPQMSHYREWLKSADLALYKAKKAGRNRTEVAA"
     gene            complement(44510..44830)
                     /gene="psiF"
                     /locus_tag="J1Y83_000581"
     CDS             complement(44510..44830)
                     /gene="psiF"
                     /locus_tag="J1Y83_000581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414918.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate starvation-inducible protein PsiF"
                     /protein_id="HBA7628766.1"
                     /translation="MKITLLVTLLFGLVFLTTVGAAERTLTPQQQRMTSCNQQATAQA
                     LKGDARKTYMSDCLKNSKSAPGEKSLTPQQQKMRECNNQATQQSLKGDDRNKFMSACL
                     KKAA"
     gene            complement(44949..46364)
                     /gene="phoA"
                     /locus_tag="J1Y83_000582"
     CDS             complement(44949..46364)
                     /gene="phoA"
                     /locus_tag="J1Y83_000582"
                     /EC_number="3.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase"
                     /protein_id="HBA7628767.1"
                     /translation="MKQSTIALALLPLLFTPVTKARTPEMPVLENRAAQGDITAPGGA
                     RRLTGDQTAALRDSLSDKPAKNIILLIGDGMGDSEITAARNYAEGAGGFFKGIDALPL
                     TGQYTHYALNKKTGKPDYVTDSAASATAWSTGVKTYNGALGVDIHEKDHPTILEMAKA
                     AGLATGNVSTAELQDATPAALVAHVTSRKCYGPSATSEKCPGNALEKGGKGSITEQLL
                     NARADVTLGGGAKTFAETATAGEWQGKTLREQAQARGYQLVSDAASLNSVTEANQQKP
                     LLGLFADGNMPVRWQGPKATYHGNIDKPAVTCTPNPQRNDSVPTLAQMTDKAIELLSK
                     NEKGFFLQVEGASIDKQDHAANPCGQIGETVDLDEAVQRALEFAKKDGNTLVIVTADH
                     AHASQIVAPDTKAPGLTQALNTKDGAVMVMSYGNSEEDSQEHTGSQLRIAAYGPHAAN
                     VVGLTDQTDLFYTMKAALGLK"
     gene            complement(46465..46725)
                     /gene="iraP"
                     /locus_tag="J1Y83_000583"
     CDS             complement(46465..46725)
                     /gene="iraP"
                     /locus_tag="J1Y83_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraP"
                     /protein_id="HBA7628768.1"
                     /translation="MKNLIAELLFKLAQKEEESKELCAQVEALEIIVTAMLRNMAQND
                     QQRLIDQVEGALYEVKPDASIPDDDTELLRDYVKKLLRHPRQ"
BASE COUNT        11995 a        12317 c        11535 g        10997 t
ORIGIN      
        1 accattgaaa ataaacgtta ttttaataac ggaaaaatta aagatggtga atgccctcat
       61 ttttatgaag atggaaaaat caaacagcaa catagctatc tgaatagtaa actcgatggt
      121 ccggcatatg aatattatcc tgatggcaaa ctcatgcagg aaaatttcta tcagcaaagt
      181 gagttgattg gaaaagatac cagttactac caatcaggaa aaataaactc tatacacaat
      241 agaaattccc gtggtcagta tgatggaatt aacgaacgtt atagtgaaga gggtaagtta
      301 ctgtctaaat ccgtctataa agacggaaaa caaatttctg ttcaaacctg gtatgaaaat
      361 gggcagaaag aagaggagaa acattttgat gtgcaaggcc aattaaatgg tttggttaag
      421 caatggtata aaaatggaaa tcttgcaaaa tcacaaaact ataaacatga catactcgat
      481 ggtgatagtg aagagtggta cgaaaatggc atccctgaga gtctgtatcc ttataagaat
      541 ggaaaaaccg acggcgttgc caaatcatgg aataagtatg gtaaattaac ctattcaatc
      601 gaatacaaaa atggcgtaga gaatggggta tatcgtaact ggagcaaaaa cacaggtaaa
      661 ctaaccaaag aaacccagta cgtcaatggt atcaggcagg gcgtggaaaa agagtttaac
      721 gatcgcacgg gtaaactatt aaccgccacg caatacgtta ataataaaag aaatggcacg
      781 gaggaaactt atgatcaaaa tggaataaaa tatattacgt gctatcaaaa tgatgaggaa
      841 ttatcgtctt tatacactcc aacacaaata aaagataatg caacaaaagg caactccagc
      901 gctcaattta cattaggaaa atacgaattc acctgtgcaa atatagatga agggattaaa
      961 tggttaacca aatcggcaga gcaaaaaaac actgatgcga tatacttttt agctacagcc
     1021 tacaaaggaa atggcatacc tgcaaacaat gaaaaataca taacatatct gcaacaagca
     1081 gcaatgctgg gcaatagtaa tgcacaggcg gaaattggct atttatatct catcggaaaa
     1141 gaactaccac agaatctgcc tgatgctggt gtctggttta aaaaagcagc tgctcagggt
     1201 aattttgttg cacatttcta cctgggaaga atgtatcaaa atggtgatgg cgttgagaaa
     1261 aatatggaaa aagccaggtt tcatctatct aatgcagcag aaggtggaat aaaacctgcg
     1321 cttaaagcat taaatgaact tgaacatcaa acaaaataaa gcgttcaggg accatgtaag
     1381 gttcattaaa cagaaaagga tatggattgc gatgaaagtg aaatctactt tgttattaat
     1441 aattttatct gtgttttatt ctcatcaggt tttatccagc gcccccactc agccggatgt
     1501 ttattctgtc gtcgagcaaa aacttaactc tgctttacct ctgaaagaaa atactacctt
     1561 cagatcgcag gctgaatggt tcaatttgca atataaatta ttagtatctg gctaccctga
     1621 acgtgcttac aatttactga gcaaacttga aacagaatcg aaaaacgcta aatattatct
     1681 agatttaact gcctccagag tcgctgaaga aggagcacca gagactgcct taactttttt
     1741 atcaaaaatt gggttaaata aaccggcaac tcttacatcc tttgagagct atatcaatag
     1801 ctggataaaa agcaaacaac ccgataaggc tatgcaactt ctcagtctcg atgacaatgc
     1861 cctacattat tatttaccta ctgtgttagt cgcatatcag agcgatcctg acaaggcaat
     1921 aagtatttat aataatgtat atggtgacaa tgttattata ccctatcagc aagccaccat
     1981 gttacttaca gttgcggagg gttatcaaac aaaaggcgat ataaaaaacg ctattcatta
     2041 tgccgataat gcgctgaata tgtttggcag tcgtgaatcc cattatggtg atgagtattt
     2101 gaaactcatg aatatctacg ctaccaatgg caacaaagaa aaagcggtag ttttatctca
     2161 acgtctacaa aaaaccatca ccgaatctca atcgaattac ttaagtactc tggaaggaat
     2221 tttactgttt tatagaaata atgatatgca gcaagactat caaaacagcc tttcaaatta
     2281 catagtattt attgataaaa cctttgcctt ttccccatca acaagaagtg aattaagcct
     2341 cattaattta ttaaattcat taaatgaggt ggaattgatg aaaaaacgtc tggcgcttct
     2401 gatgacagca ccagaataca cttgttatga tagtcagtat tgttatgaag acaaagtcag
     2461 cgcattaaaa accctgcata gtcatcagga taatgagcta atggacaaat atttcacgac
     2521 tcttcttaaa gagatcagta atcaaccatt agaagactgg gatataataa taagtaatac
     2581 cggtgaaaaa tttagtgagg tgggactaac caaatatgct caacatctcg catctttcgc
     2641 tgaagaattt tatataaaag agcaaaaatc acattctgaa agtgaaatga gaggactatt
     2701 ttcatctctt gccgaaatat atagttttgg taacgatact gccagcgcaa accgagtgtt
     2761 gcatcaacat gtccctgcct tcaatacaga tcatatgatt caatattata taaatgccaa
     2821 agaatggaac aatgccaggg aattattgat aaaagaggaa atgttgggta tgcataatct
     2881 gatattgttg gagaacattt gtatgcagaa aactgcacaa tgcatggcac acattacctt
     2941 tacgctcaac aaattgacaa cacaacctgc aataacaaaa aaagatagcg tgggtaatga
     3001 gcaactttat cagattggca aaatctacca taagctggga gttaaaccag agccaaagca
     3061 gcaggcttta attcagtcgc tttatgacca ggcatcagga agcacctctt caactcaata
     3121 aacacatggt caaaagggcc accacggtgg ccctgacaat caatagctat atccagaaag
     3181 ttaatcgcgg aagtttttga actggaacgg ctgacccaga tcgccaccac gtaccatcgc
     3241 catgacagcc tgtaaatcat cacgagattt acccgttacg cggatctcat cgccctgaat
     3301 ttgcgcctgc actttcagtt tgctgtcttt gatcattttg acgattttct tctgagtcgc
     3361 gctctcaatg ccctgtttca gtttcgcttc cacaaaccag gttttaccgc tatgaacgat
     3421 attttccggt acatccagtg aactgccttc aatgccgcgc ttcagcaact tggcacgcag
     3481 aatatccagc aactgattga cctggaagtc ggactcgctc aacactttga tggttttgct
     3541 ggcgtcattc agctcaaatg aggcttcaac gttacggaag tcaaaacggg actccacttc
     3601 gcggctcgcg ttatcgaccg cgttacgtgc ttcctgaaga tcaacttcag agacaatatc
     3661 gaaagatggc atcttttcct ctcccttcat ttttgatgcg aagcataata cctgcaaagt
     3721 taaataacca acagtcctgc accaggacgt atcccttaat tgtgcatagt cgcaacagca
     3781 tgcacaatta agggatagcc ttattgaaca cccggagtgg ttgcgggtga ggaggaacaa
     3841 tgaaaattac cgtattggga tgcggtgcct tagggcaatt atggcttaca gcactttgta
     3901 aacagggtca cgacgttcag ggctggctgc gcgtaccgca accttattgt agtgtgaatc
     3961 tggttgagac agatggttcg atatttaacg aatcgctgac cgccaacgat cccgattttc
     4021 tcgccaccag cgatctgctc ctggtgacgc tgaaagcatg gcaggtttcc gatgccgtca
     4081 aaagcctcgc gtccacactg cctgtaacca cgccaatact gttaattcac aacggcatgg
     4141 gcaccatcga agagttgcaa aacattcagc agccattact gatgggcacc accacccatg
     4201 ccgcccgccg cgacggcaat gtcattattc atgtggcaaa cggtatcacg catattggtc
     4261 cggcacggca acaggacggc gattacagtt atctggcgga tattttgcaa accgtattgc
     4321 ctgacgtcgc gtggcataac aatattcgcg ctgagttgtg gcgcaagctg gcagtcaact
     4381 gtgtgattaa tccactgacc gccatctgga attgcccgaa tggtgaatta cgtcatcatc
     4441 cgcaagaaat tatgcagata tgcgaagaag tcgcggcagt ggtcgaacgc gaagggcatc
     4501 atacttcagc agaagatttg cgtgattacg tgatgcaggt gattgatgca acagcggaaa
     4561 atatctcgtc gatgttgcag gatatccgcg cgctgcgcca caccgaaatc gactatatca
     4621 atggttttct cttacgccgc gcccgcgcgc atgggattgc cgtaccggaa aacacccgcc
     4681 tgtttgaaat ggtaaaaaga aaggagagtg aatatgagcg catcggcact ggtttgcctc
     4741 gcccctggta gtgaagagac tgaagccgtc accactatcg atctgctggt tcgcggcggt
     4801 atcaaagtca ccactgccag cgtcgccagc gatggtaacc tggcgattac ctgctcgcgc
     4861 ggcgtgaagc tgctggcgga tgcgccgctg gttgaagtgg ctgatggcga atatgacgtg
     4921 attgtgctgc ctggtggcat caaaggcgca gagtgttttc gcgatagcac cctgctggtt
     4981 gaaaccgtta aacagttcca ccgttccggg cgtatcgtcg cggctatttg cgccgcgcca
     5041 gccaccgtgc tggtcccgca cgatatcttc ccgattggca atatgaccgg cttcccgacg
     5101 ctgaaagaca aaatacccgc cgaacaatgg caggacaagc gcgtcgtctg ggatgcacgg
     5161 gtaaaattgc tgaccagcca ggggccaggt acagctatcg actttggtct gaaaattatc
     5221 gacctgctgg ttgggcgcga gaaagcccat gaagtggcat cacaactggt gatggcggca
     5281 gggatttata attattacga gtagtgtcgg atgcggtaaa cgtcgcatct gaccagatgc
     5341 gacgttacaa ccacatatta cgggcgatac accttcacat tgttaaagcc ctgctcgcgc
     5401 agatagagcg cctgcaggcg gctcatcacc ccgcgctcac accacaacag ccaggttttg
     5461 ctctggtcga gatcgccaaa tttggtgctc agtttataga acggcagaga aaccacatca
     5521 atcccttcga ctttcagtgg cttatcttcc tgttcatcga tagaacggat atcgaggatc
     5581 acgtcgttcg ggccgaagcc attgacggtt tccacttcca ccacttcctg ctcggtctgc
     5641 tgggcgattt cgcggatatc aacgttattc gcttcctcca ccactttatc gagaatgctg
     5701 aagtcgaact tctcttcttc cgcttcaatc ttcgatttaa ccgctttcac cgtcgggctt
     5761 ttggaaatca cgccgcaata ttccggcatc gtgcgggcaa agtcttcggt gccaatctgg
     5821 cgagccaggt tgatgatgtg ctctttgtcg taagagatca gcggacgcag gatcagcgta
     5881 tcggagacgt tatcaatcag gcgtaggttg gtcagcgtct ggctggacac ctggccgagc
     5941 gcttcaccgg taaccagcgc ctgtacgccg taacgttctg ccactttgga cgcggcacgc
     6001 accatcatac gtttgaggat aacgcccatc tgaccgtcgt cgattttctc gagaatttcc
     6061 ccgacgaccg gttcgaaatt aatagcgaca aaacgcacgc ggtgggagct gccaaagcgg
     6121 ttccacagat aatgcgccac ctgacgtacg ccaatttcat gcgccgcgcc gccgaggtta
     6181 aagaagcagt aatgcacgcg gcagccgcga cgcatcaaca tataactgga aacaccggag
     6241 tcgaaaccac cggaaatgag cgacaacaca tcttcctggg tgccgatcgg gaaaccgcca
     6301 ataccttcgt agcggccttt aatcagcagc agacgatcgt cttccacttc cagatggaca
     6361 gtcacttccg gattggtcag cttcacgcgc gcggattcaa tatgctgatt taaaccgccg
     6421 ccgacgtaac gttccacatc aatcgagcta aaatcatgtt tgccgcggcg cttcacgcgt
     6481 acgcaaaagg ttttgccttc cagctgatcg cgatattgaa ccaacgcttt ctcgaaaata
     6541 tcgtgcatgt cggtaaacgg cacgtcttcg acttcgagaa tatggtggat acccggaata
     6601 cgggtcaggg cgtcgcgaat agccagacgc tggttttcat cttttgcgcg aacttcgatg
     6661 ttatcccagt ggcggacgac agcgagcatc tcatcatagt gctttaaaac gttacgaatg
     6721 ttcccggtaa ggatttttat aaagcgcaag cgcacagatt ggcttttgat ggtgatttcc
     6781 gggaacaatt taatgataaa cttcatggcg gcaatggttc gttggcaagc cttaagcgac
     6841 ttgtataggg aaaaatacag cagcccacac ctgcggctgc atccaggcgc ggaagtatac
     6901 cactaacatc gctttgctgc gcacatcacc ttaccattgc gcgttatttg ctatttgccc
     6961 tgagtccgtt accatgacgg ggcgaaaaat attgagagtc agacattcat tatgccgaag
     7021 aaaaatgagg ctcccgccag ctttgaaaag gcgctgagcg agctggaaca gattgtaacc
     7081 cgtctggaaa gtggcgacct accgctggaa gaggcgctga acgagttcga acgcggcgtg
     7141 cagctggcac gtcaggggca ggccaaatta caacaagccg aacagcgcgt acaaattctg
     7201 ctgtctgaca atgaagacgc ctctctaacc ccttttacac cggacaatga gtaatggact
     7261 ttccgcagca actcgaagcc tgcgttaagc aggccaacca ggcgctgagc cgttttatcg
     7321 ccccactgcc ctttcagaac actcccgtgg tcgaaaccat gcagtatggc gcattattag
     7381 gtggtaagcg cctgcgacct ttcctggttt atgccactgg tcatatgttc ggcgttagca
     7441 caaacacact ggacgcaccc gctgccgccg tagagtgtat ccacgcttac tcattaattc
     7501 atgatgattt accggcgatg gatgatgacg atctgcgtcg cggtttgccg acctgccatg
     7561 tgaagtttgg cgaagcaaac gcgattctcg ctggtgacgc tttacaaacg ctggcgttct
     7621 cgattctaag cgatgccgat atgccggaag tgtcagatcg cgacagaatt tcgatgattt
     7681 ctgagctggc gagcgccagc ggtattgctg gaatgtgcgg tggtcaggca ttagatttag
     7741 acgctgaagg caaacacgta cctctggacg cgcttgagcg tattcatcgt cataaaaccg
     7801 gcgcattgat tcgcgccgcc gttcgccttg gtgcattaag cgccggagat aaaggacgtc
     7861 gtgctctgcc agtactcgac aagtacgcag agagcatcgg ccttgccttc caggttcagg
     7921 atgacatcct ggatgtggtg ggagatactg caacgttggg aaaacgccag ggtgccgacc
     7981 agcaacttgg taaaagtacc taccctgcac ttctgggtct tgagcaagcc cggaagaaag
     8041 cccgggatct gatcgacgat gcccgtcagt cgctgaaaca actgactgaa cagtcactcg
     8101 atacctcggc actggaagcg ctagcggact acatcatcca gcgtaataaa taaacaataa
     8161 gtattaatag gcccctgatg agttttgata ttgccaaata cccgaccctg gcactggtcg
     8221 actccaccca ggagttacga ctgttgccga aagagagttt accgaaactc tgcgacgaac
     8281 tgcgccgcta tttactcgac agcgtgagcc gttccagcgg gcacttcgcc tccgggctgg
     8341 gcacggtcga actgaccgtg gcgctgcact atgtctacaa taccccgttt gaccagttaa
     8401 tctgggatgt ggggcatcag gcttatccgc ataaaatttt gaccgggcgc cgcgacaaaa
     8461 tcggcaccat ccgtcagaaa ggtggcctgc acccgttccc gtggcgcggc gaaagcgaat
     8521 atgacgtatt aagcgtcggg cattcatcaa cctccatcag tgccggaatt gggatcgcag
     8581 ttgctgccga gaaagaaggc aaaaatcgcc gcaccgtctg tgtcattggc gatggcgcga
     8641 ttactgctgg catggcgttt gaagcgatga atcacgcggg cgatatccgt cctgatatgc
     8701 tggtggttct caacgacaat gaaatgtcga tttccgaaaa tgttggcgcg ctcaacaacc
     8761 atctggcgca gctgctttcc ggtaagcttt actcttcact gcgcgaaggc ggaaaaaaag
     8821 ttttctctgg cgtgccaccc attaaagagc tgctcaaacg taccgaagaa catattaaag
     8881 gcatggtagt gcctggcacg ttgtttgaag agctgggctt taactacatc ggcccggtgg
     8941 acggtcacga tgtgctgggg cttatcacca cgcttaagaa catgcgcgac ctgaaaggcc
     9001 cgcagttcct gcatatcatg accaaaaaag gtcgtggtta tgaaccggca gaaaaagacc
     9061 caatcacctt ccacgccgtg cctaaatttg atccctccag cggttgtctg ccgaaaagta
     9121 gcggcggttt gccaagctat tcaaaaatct ttggcgactg gttgtgcgaa accgcagcga
     9181 aagataacaa gctgatggcg attactccgg cgatgcgtga aggttccggc atggtcgagt
     9241 tttcacgtaa attcccggat cgctacttcg acgtggcaat tgccgagcaa cacgcagtga
     9301 cctttgctgc gggtctggcg attggaggct acaaacccat tgttgcgatc tactccacct
     9361 tcctgcaacg cgcctatgat caggtgctgc atgacgtggc gattcaaaag cttccggtcc
     9421 tgttcgccat cgaccgtgcg ggcattgttg gtgctgacgg tcaaacccac cagggcgctt
     9481 ttgatctctc ttacctgcgc tgcataccgg aaatggtcat tatgaccccg agcgatgaaa
     9541 acgaatgtcg ccagatgctc tataccggct atcactataa cgatggcccg tccgcggtgc
     9601 gctacccgcg cggcaacgca gttggcgtgg aactgacgcc actggaaaaa ctgccaattg
     9661 gcaaaggcat tgtgaagcgt cgtggtgaga aactggcgat ccttaacttt ggtacgctga
     9721 tgccagaagc ggcgaaagtc gctgaatcgc tgaacgctac gctggtcgat atgcgtttcg
     9781 tgaaaccgct tgatgaagcg ttaattctgg aaatggccgc cagccatgaa gcgctggtta
     9841 ccgtagaaga aaacgccatt atgggtggtg caggcagcgg cgtgaacgaa gtgctgatgg
     9901 cccatcgtaa accagtaccc gtgctgaaca ttggtctgcc ggacttcttt attccacaag
     9961 gaactcagga agaaatgcgc gccgaactcg gcctcgatgc caccggtatg gaagccaaaa
    10021 tcaaagcctg gctggcataa tccctactct actcctgcta tgcttaagaa attattcatg
    10081 gcaggagtgg cagcatgcaa tacaacccct taggaaaaac cgaccttcgt gtttcccgac
    10141 tttgcctcgg ctgtatgacc tttggcgagc cagatcgcgg taatcacgca tggacactgc
    10201 cggaagagag cagccgtccc atcattaaac gtgcgctgga aggcggcata aatttctttg
    10261 acaccgccaa tagctattcc gatggcagca gcgaagagat cgtcggtcgc gcactgcggg
    10321 atttcgcccg tcgtgaagac gtggtcgttg cgaccaaagt gttccatcgc gttggtgatt
    10381 taccggaagg attatcccgt gcacaaattt tgcgctctat cgacgacagc ctgctccgtc
    10441 tcggcatgga ttatgtcgat atcctgcaaa ttcatcgctg ggattacaac acgccgatcg
    10501 aagagacgct ggaagccctg aacgacgtgg taaaagccgg gaaagcgcgt tatatcggcg
    10561 cgtcatccat gcacgcttcg cagtttgctc aagcactgga actacaaaaa cagcacggct
    10621 gggcgcagtt tgtcagtatg caggatcact acaatctgat ttatcgagaa gaagagcgcg
    10681 agatgctgcc actgtgttat caggagggcg tggcggtgat tccatggagc ccgctggcgc
    10741 ggggccgtct gacgcgtccg tggggagaaa ctaccgcacg actggtgtct gatgaggtgg
    10801 ggaaaaatct ctataaagaa agcgatgaaa atgacgcgca gatcgcagag cggttaacgg
    10861 gcgtcagtga agaactcggt gcaacacgag cacaagttgc gctggcctgg ttattgagta
    10921 aaccgggcat tgccgcaccg attatcggaa cttctcggga ggaacagctt gatgaactgc
    10981 taaacgcggt ggatatcact ttgaagccag aacagattgc cgaactggaa acgccgtata
    11041 aaccgcatcc tgtcgtagga tttaaataag agtccaggcc tgatgagacg tgacaagcgt
    11101 cacatcaggc atcggtgcac aactacgaca gaatacccag tggccagtga tgaccgataa
    11161 aatacaggat gccagcggag atcaccccgg cgacaatatc atctatcatg attcccatgc
    11221 cgccatgtac attgcggtca aaccagcgga tcggccacgg tttccacata tcgaggatac
    11281 ggaaaatcac aaacccggct gcgacccact gccagtcatt ggtcggcagc gccatgagcg
    11341 tgatccacat accgataaat tcgtcccaga caatgctgcc atgatcgtgc acgcccatgt
    11401 ctttcgccgt ttgatgacaa agatagacgc cgatacagat ccccagcatt accaccagcg
    11461 aatagagctg ccagggcaaa aaagtcatca gataccagaa gggaatcgcc gccagcgacc
    11521 ccatcgtgcc aggaacgatc gggcttaatc cacttccgaa tccgacagca agcagatgcc
    11581 acggattact cattttcagg cgacttttcg cgacatcttt atggcgtggc aaaatggtca
    11641 tatcctttcc agtctaacgt gacaggttcg ccgtcacgaa taaaacaaag cccttcgata
    11701 tcggcggtca tttgcccgat acaggtaaac ggtacaccca ggtgtccgag agccacatcc
    11761 agcgcgccac ggttcagttc cggcaccgtg aaacacaact cgtaatcttc accgccagag
    11821 agcgcccagc gcagcgcctg ttccggttca acatggcgag aaagcgcatc agaaaacggc
    11881 agcaatgcca ggtcaatacg tgcgccgcag tcgctggctt tcacgatatg cccgagatcg
    11941 gaaatcagac cgtcagagag atcgatagct gaatttgcca gatcgcgcag tgcctgcccc
    12001 tgtaaaatac gcggcgacgg acggagatga cgtttgatca agtagtccgc atctttagca
    12061 tcggcaacct gcaaacggtt ttgcaaaatc gccagcccgg cagcgctatc gcccggtgta
    12121 ccggtcacat agatccagtc acccggtttc gccccagagc gcgttaaggc tcgtcccatc
    12181 ggaacaaagc cgtggatacc caacgtcatt gataatggcc cacgcgtggt atcgccgcca
    12241 atgagttgca tatcgtaata attgagaaga tcaaacaagc tgtcgctgaa ggactcaagc
    12301 cacgcttcgt ctacgtccgg taaggttaat gccagcgtca gccaggccgg atcggccccc
    12361 atcgctgcca gatcgcttag gttcaccgcc agtgctttat aagccagatc agcaggatcg
    12421 atatcaggga ggaagtggtt gcccgccacc agcgtatcag tgctgatcgc cagggtctgt
    12481 ttctcgggga tattgagaag agcgcaatcg tcgccgatgc ccagttcgac atcaagacga
    12541 gaacttctta cacggtcaaa ataacgggca atcagggaga actcgccaca tgccatacgt
    12601 tatgcctcag caggtaaaaa agaaaaggcc ggcgatcgcg gaacggtctt ccgtgaatct
    12661 accggcctgg atattacttt ttgttagggc gaatcacagg tgctgcttta tcgagtacgc
    12721 cgttgacgaa cttatggctg tcttctgcgc cgaaagattt cgccagttcg atcgcttcgt
    12781 taatggccac tttgtatggc acatcgctac gtttagacag ctcgtacagc gcaatgcgca
    12841 gtactgcttt ttctacctgg cccagttctt ccagcagacg ggacaggtac ggcttcatca
    12901 gtccgtcgag gtatgcggtg ttagtcgcca ccccggccag cagctcacgg aagtacagga
    12961 cgtcaacgtc ttttacatcc tgttcagcca ggaactggta ttcaacatca gcgatgtcgt
    13021 tctgggacaa ctgccaggag tagagcgcct ggacggcaca ctcacgagcg cggcgacgag
    13081 cagcaggttt cacggatttc cccttactaa tttcaggcct tgatggcttt caatacatta
    13141 atcatttcaa gcgcggtcag tgcagcttct gcacctttgt tgccagcttt ggtgccagca
    13201 cgttcgatcg cttgttcaat gctttcagtg gtcagaacac caaaagcaac cggaatttcg
    13261 ctgtcctggg caacatgcgc caggccgttg cttgcaccac cagcgacata ttcaaagtgg
    13321 gcagtgccac cacgaataac cgtacccagc gcaatcaccg cgtcgtattt accggtttta
    13381 gccagtgcac ccgccgccag cggcagctca taggcacctg gcacccaaac aacggtaatg
    13441 ttttcatctt ttacctgacc gatacgtttc agtgcgtcaa ttgcaccttc cagcaggctg
    13501 tcattgataa agttgttgaa acgcgcgatg gtgatggcga cgcgagcgtc cggggtagca
    13561 acgttagctt caataatgtt cattttcttc cttcgggttc gagtatggcc ccgcaggggg
    13621 gcggatttta gcataatatt tcgtgcgctg cttccctttc gagccgggag atcatgcacc
    13681 cactaaatgc aggcaaacat ccgggcctac atgacgtatc tctttgaatt taaattgggg
    13741 agcgtcggct aatttctcaa gccctggcag cgtgcataat ccacgggcgt cgttgcctaa
    13801 tagtttaggt gcgatataga caatcagctc atcgactaaa cccgcctgta gcaatgcgcc
    13861 agcgagcgtt ggccccgctt ccacccagat gctgttaatt tgctgtttac ccagttgcat
    13921 catcagtaca accagatcca gatgaccttt atgctctgga atcagcaagg tacgcaccgt
    13981 ttccggccac tcacgagaat catcctgcgt acgcgcgaac caggtttcgc cgggctgctg
    14041 cacaatgcga tgttccggcg tcacgcgatt ttggctatca atcacaatac gtatcggctg
    14101 acggagattt tgttgcggat agagcgcctg agtttgttca tccagttcag accaacgcac
    14161 tgttaaggct ggatcatccg ccagcaccgt ggcgctgctg gttaaaatgg catgactttg
    14221 tgcgcgcagt cgttgtacgt cgcgcctcgc ctgaggtgaa gtgatccact ggctttcgcc
    14281 actcgccatc gccgtgcgac catcaagcga tgcgccaagt tttaactgaa tataaggaaa
    14341 gccggtgcgc atccgcttga gaaagccttt attcaattgc tcagcttcac tcatcatcag
    14401 cccgtggctg acgtcaatgc cagcctgttg cagacggtaa agtccacgtc cagcgacctg
    14461 cggattgggg tcttgcattg cagcaaccac gcacgccacg cccgcagcaa ttaatgcatc
    14521 acaacacggt ggcgtacgac catgatggct acagggttcg agtgtgacat acgccgttgc
    14581 gcctttggct ttttcgccgg ccatgcgcaa cgcgtgtacc tcggcatgcg gttcacccgc
    14641 tcgttgatgg taaccttcac cgacaatttc gccatctttg acaatgacgc acccgacatt
    14701 cgggttggga tgcgtggtaa aacgtcctcg ttgcgccagc tttagcgccc gcgccatgta
    14761 atactcgtcc tgcacggctt agtcctccag gcgcgcgatc tcttcgccaa attctttgat
    14821 atcttcgaaa ctgcggtaga cagaggcaaa acggatatag gcgactttat cgagcttttt
    14881 caattgctcc atcaccagat tgccaatcat cttgctcggc acttcgcgct caccggtggc
    14941 gcgcagctgc gattttatat gattgattgc catttcgacg tcatcggaac tcactggacg
    15001 tttttccagc gcccgcagca ttccgctacg taatttctct tcattaaacg gttcacgcac
    15061 gtcgttgctt tttacaacac gcggcataac cagctccgcc acttcaaagg tggtgaaacg
    15121 ttcattacac accagacact gccgacggcg gcgtacggat gaaccctcgc ccacgagacg
    15181 agagtcaatt accttagtgt ccacggcgaa acagaatggg caatgcatac ggcgtcctga
    15241 ccaggtggtt aacagaaatc tattttaccc tgaactgacg atacaacaaa ggcgcaacgc
    15301 ttttgagata aaggatcgat gctttcactg cttttaccct ctaccattag gctaattgcg
    15361 acgagaaagg gacgacctat gaatactaac gtttttcgac tgctcctgct gggaagcctg
    15421 ttcagcctta gcgcctgtgt gcagcaaagt gaagtgcgac agataaaaca cagcgtcagc
    15481 acgctgaacc aggagatgac gcagctcaat aaagaaacgg tcaaaatcac tcagcaaaac
    15541 aagctgaatg caaaatccag cagtggggtt taccttctgc ctggagcaaa aacaccagca
    15601 agactggaaa gccagatcgg tactttacgt atgtcgctgg tgaatattac gcctgacaca
    15661 gatggcacta ccctgacgct acgtattcag ggcgaatcca atgacccttt acccgcattc
    15721 agcggaaccg ttgaatatgg gcaaattcag ggaacaatag acaactttca ggaaatcaat
    15781 gtgcagaatc aattgattaa cgcacctgcc agcgtcctcg cccccagcga tgttgatatt
    15841 ccgttacagt taaagggtat gtctgtggat cagttaggct ttgtacgtat ccacgacatt
    15901 caacctgtta tgcagtaaac gtattgcggg gcgatattgt gcgtcccgca acatcttttt
    15961 ccgtcatttt gttactccgc ttacatcacc tggattgata gtaaaagttt gcaacaaggg
    16021 tgaaagtcag tacaatcccc gcccgaatgt gtgtaaacgt gaacgcaatc gattacgtaa
    16081 atgatagaac tgtgaaacga aacatatttt tgtgagcaat gatttttata ataggctcct
    16141 ctgtatacga aatatttaga aacgcaattt gcgccttttt cactcccgca agggattttc
    16201 aaacagtggc atacatatga aaaaaacatt actggcagcc ggtgcggtac tggcgctctc
    16261 ttcgtctttt actgtcaacg cagctgaaaa cgacaaaccg cagtatcttt ccgactggtg
    16321 gcaccagagc gttaacgttg tcggaagcta tcacacccgt ttcggaccgc agctccgcaa
    16381 cgatacctac cttgagtacg aagcattcgc taaaaaagac tggttcgact tctatggtta
    16441 tgcggatgcg ccggtattct tcggcggtaa ctccgatgca aaaggtatct ggaaccacgg
    16501 ttctccgctg tttatggaaa tcgaaccgcg tttctccatc gacaagctga ccaatactga
    16561 ccttagcttt ggtccgttca aagagtggta cttcgcgaac aactacattt acgacatggg
    16621 tcgtaataaa gatggccgcc agagcacctg gtacatgggt ctgggtaccg acatcgatac
    16681 tggtctgccg atgagcctgt ccatgaacgt ctatgcgaaa taccagtggc agaactacgg
    16741 cgcagcgaac gaaaacgagt gggacggtta ccgtttcaaa gtgaagtact ttgtgccgat
    16801 taccgatctg tggggcggcc agctgagcta catcggcttc accaacttcg actggggttc
    16861 cgacttgggc gatgacagcg gttatgcgaa caacggtatt aaaacacgta ccaataactc
    16921 tattgcgtcc agccatattc tggcgctgaa ttacgatcac tggcactact ctgtcgttgc
    16981 ccgttactgg cataacggtg gtcagtggaa tgacgatgca gaactgaact tcggcaacgg
    17041 caacttcaac gttcgctcta ccggctgggg tggttatctg gtagtaggtt acaacttcta
    17101 attgtgagaa tgccggattt catatccggc attttttatc acttcttctt gttcatcatc
    17161 gctttcaaat cagcgaatgg gttgtacttc gcttcaccga catctttctg cgcatcttcc
    17221 cctgcgataa cggtcgtacc atactgatcc gcttcggtgt atttcgaatg ctcatgatcg
    17281 tggcaataga gacaaagtaa ttcccagtta ctgccatctt ccgggttatt agtatggtcg
    17341 tgatcaatgt ggtgaacggt aagttcacgc aggttggaat aaacaaactc gcgggaacag
    17401 cgaccgcaga cccacggata gatttttaat gctttttcgc gatagccgct ttctaaccgc
    17461 gcatagtttt ttgggatgat agccatgaaa aagttacctg gatgaaatgt agggttacgc
    17521 gcagtttccc aagatgcggg gattttcgca atcaccttac atcaaaaaaa atcccgatct
    17581 tctgaccggg attttcaaca tcagctgaaa cttgattaag gcagaattga cggctgatcc
    17641 gccccttctt tttccacttt ctgctgcaac atgtgttcgc gcttcatacc cagtttcaga
    17701 gccagcgcag atgccacata gatggaagat gcagtaccga tggaaacacc gataagcatg
    17761 gtcagcgaga agccttccag taccggacca ccgaagaggt acagcatcag gataaccatc
    17821 aaggtagtac cggatgtgat caaggtacgg tgcagcgtct gggtcaagga cacgttaaag
    17881 atttcgtaag gcgtaccgcg acggatcttg cggaagtttt cacgaatacg gtccgatacc
    17941 acgatactgt cgttaagcga gtaaccgata accgacatca acgatgccac aatggtcagg
    18001 tcaatctcga tatggaataa cgacaaaata cccagcgtaa tgataacgtc gtgcgccagc
    18061 gcaataacaa cccctgccgc cagtcgccac tcaaagcgga aacctacgta cacgaggata
    18121 gacagcagcg ccgccatcaa cgccatcgca ccggtttgcg caaggtctgc ccccacgctc
    18181 ggaccgacga actcaatacg cttcactgcc gcattctgat tggtggattc gttaatcacc
    18241 ttcagaacct ggctgcccaa cacctgaccg ccggtttcgc cttcagcagg cggcatacgg
    18301 accatgatgt cgtggctgct accaaagttt tgcagcatcg gctcttcaaa accggctttt
    18361 tgcaatgcat cacgcattac gtcaatttca gccggttttt cgagcgtaat ttcaataacc
    18421 gtaccaccgg taaaatccag cccccagtta aagccgcgca cgcccataat aacgatagcg
    18481 gcgattaaca gcagaccaga gatgccgaaa gcccagtagt cccagcgcat aaagtcatag
    18541 actttacggc cgtggtttag ttgttcaaca gtatattcct gtgccacatc gcactcctca
    18601 gattgacagc ttcttgacgc gcttgccgcc atatagcagg tttacgatgg cacgcgtacc
    18661 gacaatcgcg gtaaacatcg acgtcgccac accgataccg gtagtaatcg cgaacccttt
    18721 aattgccccg gtacccactg catacaggat gatgacttta atcagcgtgg tgatgttcgc
    18781 atcgaagatg gaactgaatg cgccacgata accttcatca attgcctgtt gaacagtacg
    18841 tccgttgctc aactcttctt taatacgttc gttgatcagt acgttcgcat cgaccgctac
    18901 cgcaagggtt aagacgatac ccgcgatacc tggcatactc agcgttgcgc ctggcaacag
    18961 cgacataata ccgacaatca agatcaagtt ggcaatcaga gcactggtcg caatcagacc
    19021 aaacttctta tagaagatga tcatgaacag aatagaaacc agcagaccgg caaggcaagc
    19081 ctccagcccc tgctcaatgt tctgcatacc cagggttggg ccaatggtac gttcttcaac
    19141 aatctgaatc ggcgcgatca acgcaccggc acgcagcagc agtgaaagct gacgcgcttc
    19201 gcttgggtta ttgatgccag taatacggaa gctgttaccc agacgagact ggatgttggc
    19261 gatattaatc acctcttcct gtttcaccag aaccgcacga ccgtttgcat ctttcttacc
    19321 gctgtctttg tactccacaa acagggttgc catcggtttg ccgatgttgt ccttggtgaa
    19381 gttagacatg atgttaccac ccgcgctatc gagcgagatg ttaacctgcg gctggttgta
    19441 ttcgtcctgg ctggaagtgg agtcggtgat atggtcaccg gtcagaatta cgcgtttgta
    19501 cagcacaacc ggctgacctt cgcgggtctg tttcacttca gagtcgcccg gtacgcgacc
    19561 ggatgccgca gcggcctgat caacgttggt gtttaccaga cggaattcca gcgttgccgt
    19621 cgcacccaga atctctttcg cacgcgcagt gtcctgaata cctggcagtt caacaacgat
    19681 acggtcagca ccctgacgct gaaccaccgg ctccgccacg ccaagttggt ttacacggtt
    19741 acgcaggata ttgatgttct gctgcaccgc atattcacgc gcttcactca gacgagcatc
    19801 gctcattacc gcacgcagct ggttgctgcc ctggctgcta atcaccaggt ccggatggcg
    19861 cttgctcaga tacgcaatgg cttcatcacg agctttagca tcgcggaaag tgatgctcag
    19921 gccgtagttg ttttctttac gaacggtggt atacgggatg cccttttcac gcaggtcgct
    19981 gcgcaggcta tcgatatttt gttcctggag tttgccaagc gcggtgtcca tatccacttc
    20041 catcaggaag tgaacgccgc cacgcaggtc aaggccgagc ttcatcggct cagcatgaat
    20101 ggctgccagc cagcgcggcg ttgccggggc aaggttaagc gccacgacgt atttgtcacc
    20161 cataacgccc attaatgctt cacgagcgcg cagctgggtg tcagtggagt cgaagcgcgc
    20221 aagaatagcg ccctcttcca gtgccacaga cttagcagtt attttttctt cttgtaacgt
    20281 tttctggacc tggatcagcg tttgctcact ggcggcgact ccgcgcgcac cagtgatctg
    20341 aacagccgga tcctcaccaa acaggttggg aagcgcatac agcagaccga tgacaatcac
    20401 cacgatcagc atgacgtact tccacaaagg ataacggttt aacacggcaa ttcccttagg
    20461 gaaaaatttt aattacagcg ccttcatggt gcctttcggc agaacggcag ctacgaagtc
    20521 acgtttaata actacttcag tggtgtcatt cagtgcgata gcaatgtagc cgttttccgc
    20581 tactttggtt acgcgaccga ccaggccacc gttcgtcagc acttcatcac ctttagcgat
    20641 ggagtccatc agctttttgt gttctttggt gcgcttctgc tgtggacgca ggatcatgaa
    20701 atagaaaatc agaccgaata ccaccagcat caaaatcaaa gacatcgggc taccttgcgc
    20761 cggtgcaccc gttgccgcta ccgcatcaga aataaaaaag ctcattaaat ttccctcatt
    20821 attaatatta atcaacgttc aaaggtggta cttctcgccc ctgacgctgg taaaaatcag
    20881 ttacgaagct ctctaattta ccctcttcaa tagccttgcg taaacccgcc atcaaacgct
    20941 ggtagtaacg aaggttatga atggtgttga gtcgcgcgcc taatatttcg ttgcaacggt
    21001 cgagatgatg caagtaagcg cgtgaataat tgcgacaggt gtagcaatca cactcaggat
    21061 cgagtgggcc agtatcgctc ttatacttcg cattgcggat tttcaccacg ccatcggtca
    21121 cgaacaaatg accatttcgg gcgttgcggg ttggcattac gcagtcaaac atatcgatac
    21181 cgcgacgtac gccctcaacc aggtcttctg gtttaccaac gcccatcagg taacgcggtt
    21241 tgtctgccgg aatttgcggg catacatgct ccagaatgcg gtgcatatct gctttcggct
    21301 cacccacagc cagaccgccg acagcgtagc catcaaaacc gatatctacc agacctttaa
    21361 cagaaatatc acgtaaatct tcgtaaacac tgccctgaat gataccaaac agcgcatttt
    21421 tgtttccgag actgtcaaaa cgctcacggc tacgcttcgc ccaacgcaga gacatctcca
    21481 tggagcgttt tgcgtaatcc cagtcagcag gatacggcgt acactcatca aagatcatga
    21541 cgatatccga accaagatcg tactgaatct ccatcgactt ttccgggtcg aggaaaatcg
    21601 ggtcgccgtt gatcgggttg cggaagtgaa cgccctgttc ggtgatttta cgaatatcac
    21661 caaggctgaa gacctggaag ccgccggagt cggtaaggat tggtcctttc cactgcataa
    21721 aatcgtgcag atcgccgtgc agtttcatga tttcctggcc cgggcgcagc cacaggtgga
    21781 aggtgttgcc gaggataatt tgcgcgccag tggcttcaac ttcttccggc gtcatccctt
    21841 ttacggtgcc gtaagtgcca acaggcataa aacaaggcgt ttccactacg ccacgatcaa
    21901 agaccaggcg gccacggcgt gcgcgaccgt cggtggtgtc cagttcaaat ttcatttttt
    21961 ctccactacg tcagaaaaac agtccaacgt ttaaaccagc gccgcggaat tactccccaa
    22021 cgcgctcatt aattgcctgc ggattgtacg tgataaacat cgcatcaccg taactaaaaa
    22081 agcgatattt ctcttctacc gccgctttat aggcattcat ggtgtgttga taaccagcaa
    22141 atgccgaaac cagcataatc agcgtcgatt ccggaaggtg gaagttcgtc accagcgcat
    22201 cgaccacttt gtactggaag cccggataga taaagatttg ggtatcgtca aagaacggtt
    22261 caatgagatc gtttttcgct gcctgagccg cactttccag cgaacgtact gaggtggtac
    22321 caaccgcaat cacccggtta ccgcgcgctt tcgccgccag taccgcgtct accacatcct
    22381 gcggcacttc agcgtattcc gagtgcatga tgtgatcttc aatggtgtcg acgcgcaccg
    22441 gctggaaggt gcccgcacca acgtgcaacg tcacaaacgc catctccacg cctttggcgc
    22501 gtaatttttc cagcaaaggt tcgtcaaagt gcagtccggc ggtcggtgct gccaccgcgc
    22561 cgggtttttc gctataaacc gtttgataaa gttcgcggtc tgcatcttcg tccggacggt
    22621 cgatgtacgg cggcagcggc atatggccga tgctgttgag aatatccagc accgaacgtt
    22681 gatcattaaa ttcgacttca aacagtgcgc cgtggcgcgc ggtcattgtt gcgttaatac
    22741 tttcgtcatc gcccagcagc agttctgcgc caggttttgg cgctttcgag gcgcgaatat
    22801 gcgcaagaat gcgtttgtcg tcgagcatcc gctcaaccag cacttcaatc ttgccgccgc
    22861 tggctttacg cccaaacaga cgcgccggga tcacgcgggt attattaaaa accagaagat
    22921 cgccggggtt gagcttatcg agtaaatcgg tgaaagtacc gtgcgtcagc gcgcccgtcg
    22981 gcccgtccag cgacagtaaa cgacagctac tgcgttcagg catgggatag tgggcaatca
    23041 gggattcggg caattcaaaa gagaaatcgg taacgcgcat gacactgact cagactaaaa
    23101 taagaggcgg gtagtctagt gccggggcgc tctccctgca acctttaccc ccctttggat
    23161 agaatataat cgatgaattt tttagctcac ctgcatttag cccatctcgc ggaaagctcg
    23221 ctttccggca atttactggc tgatttcgta cgcggaaatc ctgaagaaag ttttccgccc
    23281 gacgtcgtgg ctggcattca tatgcatcgg cgaatcgacg tattaaccga caatctgcca
    23341 gaagtccgcg aagcgcggga gtggtttcgt aatgaaacgc gccgtgtcgc gcctattacg
    23401 ctggatgtga tgtgggatca ctttctgtcc cgccactggt cgcagctgtc gcccgatttt
    23461 ccgctacagg aatttacctg ttatgcccgg gagcaagtga tgactattct tccagactca
    23521 ccgccacgct ttatcaatct gaacaactac ttatggtcag agcagtggtt ggtgcgctat
    23581 cgcgatatgg atttcatcca gagcgtgtta aacggcatgg caagccgtcg cccacgtctg
    23641 gatgccctgc gtgactcctg gtacgattta gacgcccatt acgatgctct cgaaacccgc
    23701 ttctggcagt tttatccgcg gatgatggag caggcgtcac gcaaggcgtt ataagacgtt
    23761 aattcatcca caccgtagcc gaaatggcag gcaaagccag aatcccgtcc gtcagttgcc
    23821 cgtgaccttc tttgcgttgc cattgcgcga cattaagcaa cggtgacgcg ggtagcacca
    23881 cttcacaggc ttcgccacgg ttgattgcca ccagtacacg ctgctgattc agcacgcgga
    23941 caaataccac cacgttatct tccgcataca gcacctgaca gccgccacga cgcaacgcct
    24001 gacctttctt acgcagcgca atcattcgct ggtacagcgc gaataacgcc gaatcctgct
    24061 tttccacctg ccaggggaac ggcttacggc aaaacggatc gtttttgcca tccagtccca
    24121 cttcatcacc gtaataaatg cacggtacac caggccaggt gaacagccag accaccgcca
    24181 ggggcaggcg cgcaacatcc cgaccgagca gcgttttaaa tcgcgcagta tcgtggctgt
    24241 cgagctgatt aaacatacgt aattgttgtt gatgagaaag ccctgcgcgg taattatcca
    24301 tccaggccat acaggtttgg gcgtcaattt gctgcggatc gtaagagata tcggtgttgg
    24361 caagaaatcc ccacaacggg aatgtgaagc cgcgatagtt catggcggca tcttccacat
    24421 cggcctgtaa ccattgccgt gcatcgccaa aatgttcgcc gacaatatac gcatccggct
    24481 gggtttcttt cgccgcttcg gtgatcccgg caacgtgctg caaattattg cgcgccccac
    24541 ccgcttcccc cagcatatgc accacatcca gccgccagcc gtccatattc cacggcgctt
    24601 tcagccagtg gcggacaata ctgtcttccc cgcgataaat ttcattcacc agactttccg
    24661 actgataatc cagcttcggc aagctggcat agcccagcca gtcgagcgcc gtgccatcgt
    24721 cactaaacga gtaccagtcg cgccagggcg attcggggtt gtgacaagca ccacccgtcc
    24781 cgcgattgtg cctgtcaaac caggcatggg aatcgccact gtggttaaac acgccgtcca
    24841 gcaccagccg cattcccagc tgctgcgtat tgtgtcgcaa acgcaacaac gccccatcac
    24901 cgccaaactg cggatcgaca tggcgataat cctcggtatc gtatttatgc acgctgggag
    24961 ctttaaacac cggattgaga tacagcgccg tcacgccaag ttttttcaga tacggcagtt
    25021 tttcgcttat cccgtccaga tcgccgccat agaacgttga tccgcccgcc tgcgccgtga
    25081 ccggttcatc ccagtcacgc aatatgatct cttgtccggc tgcgtgatgg taataaacat
    25141 gatcctgttc agcttcacga ggaagactac gggcgaaacg atcagggaag atctgataaa
    25201 aaatctgatc cgcagcccat tgtgggccga tatccgggac atcgacggca aactgctcca
    25261 gtcgtgctgg tggcattcgg ctgaagccct gcggtgtaaa ccaacgctgg cgatcgtgcc
    25321 acagtaattt gaaactataa cgccgccgag gttgcccgct ggagagatcg atcgccgccc
    25381 gccatgcggt aacgccaggc tgcggctgac tgcgctgctt atgcatcgcc actgacgttt
    25441 cttcgttgtc gtgttctgta cgcagcataa tgcgctgcgg tgggtcttcg cccgtcagcc
    25501 acagcgtaat gagcagttga tctttgtttt gtttaacaaa tgggggcacc ggcaggtgcc
    25561 atgcatttaa catcataatt cccctgtgat gaaaatctga tcaccttgcc atagcctaat
    25621 gcaggatcac tcatcatctg aaggatattt gggggaggag gacgggggcg gataactttc
    25681 ggaatagccg gatcaggatt tcctcctgaa ccggcttaca gaacgcaagg attagtggtt
    25741 ttcagccagc tgacgatcgt ggcggcgctt aaacatccag ccaatcaaca gcacaacaat
    25801 ccacgcgaaa ccgacataca gcgagatacg cgtatcaggg tgataaccaa tcaacccgat
    25861 aataaagagc aggaaaatca aaccgccgat ggtggttgct accccacccg gtactttaaa
    25921 tttcagcgcc ttaacttctt ctggcggtaa acggcgacgg aaggcgattt gcgacagcag
    25981 gatcataatc cacacccaca ccgtggcgaa ggtcgccagc gaagcgatca ccaggaagac
    26041 gttttccggc atgatgtagt tcagatatac cgcaaacaat agcgcggtag tcatcaccag
    26101 caccgtgacc cacggaatac cacgacgcga ggttttgctg aaaatttttg gcgcgctgcc
    26161 ctgctctgcc ataccgtgga gcatacggcc tacaccaaat acgtcactgt taattgccga
    26221 cagcgaagcg gtcagcacaa caaagttaag aatgctggcg gcaaaggtaa tgcccatatg
    26281 ctggaacgtc agcacaaacg ggctaccggc agtgccaacc tgattccacg ggtagataga
    26341 cataatgacg aacagcgtac cgacgtagaa caccagaata cgcatcggca cggagttaat
    26401 cgcacgcgga atcgatttct caggatcttt cgcttcaccg gcggtaatcc cgataatttc
    26461 gatcccaccg taagcaaaca tcaccatttg caacgacatc accataccaa gccagccgtt
    26521 actgaagaag ccgccgttgc tccacaggtt atgaataccg gtcggttgcc cgccgttgcc
    26581 aatcccccag atgatgatgc cgaaaccggc gacaatcatg atgatgatag tggcgacttt
    26641 aaagaacgag aaccagaact ccagctcacc gaataccttc acgctcatca ggtttacggc
    26701 gcaaatgatc agcaccacac tcagtaccca aatccagtgc ggcaccgtcg ggaaccagac
    26761 ccccatatag ataccaaaag cggtcacatc ggcgatggcg acaataagga tttcaaagca
    26821 gtaggtccag ccggtaatgt aacctgccag cgggccgagg ttttcctgcg cataacgcga
    26881 gaaagagctg gctgccgggt tatgtaccga catttccccc agcgcacgca taatgatata
    26941 cgccgcgata ccaccgataa tataggccaa caacacgctt ggaccggcca ttttgatggc
    27001 atctgccgaa ccgtaaaaca gcccggtgcc aattgctgaa cccagtgcca taaagcgtat
    27061 gtgtcgggta cttagcccac gctttagctt gttcttactt tccatcaatt cctaacccat
    27121 caacacaata aaaaaccacg gggcatcgca gccccgtggt taaaacaaat gttcagtgat
    27181 ttagtgagcg ctggaggtca cctgacgacc tgccgcgcga tcccagataa tggccagaac
    27241 caccatcacc actgttggca ttaaccacgc cagaccttgt tcggccagcg gtaaacgctg
    27301 cgcccaggac ggtaagatat cgctgaatgc agatgccttg atcccgtcga gaataccaaa
    27361 aagcaggctg ataaacatcg gcggagcaat cacgcgggac gaattatgcc accatgagcg
    27421 tgtaaaactt aatacaacca gtgcgataca cggcggataa atggctgtca gcaccggtac
    27481 agagatctga atcagctggc tcaagccgag gttagacacc accatcgaga agccgccgag
    27541 gataaacacc agcgtacgat aagagagcgg tacgtactgg gcgaagaatt ctgcacaagc
    27601 acaggtcagg ccaaccgccg tgaccaggca ggcgatgaag attaacgccg ccagcaggaa
    27661 gctaccgccg ccgccaaagg tatgctgaac gtaagcatgc aggatcgccg caccgtttgc
    27721 agactgatcg accagcgacg cgctgtctga acccagacgg aacagcgcca ggtacagcag
    27781 agtcagacca acacccgcca tcaggccagc ccagacggta taacgggtca gcagacgcgc
    27841 ttcggtaacg ccacgagaac gcgccgcgtt aacaataacg ataccaaaca ccattgcgcc
    27901 cagcgtatcc atggtcagat agccgttgac gaagccgtta gaaaacgcag cgttttgata
    27961 agcctcagtc gccgtgctga tagaacccgc cggccagata attgcggcaa cagacaggat
    28021 gaccagcgcg ataattttca gcggcgcaag gaagttgccc acggtatcca gcagcttgcc
    28081 cggatagagc gaaaccagaa taacgatagc gaaatagacc aggctgtaaa taaacagcgg
    28141 cagcgcggaa tcacccgtca gcggcgcaat gcccacttca aaagaaacgg tagctgtacg
    28201 cggcgtagcg aaaagcggcc ccaccgccag gtaacaaact gttgccagca gtacgccagc
    28261 gactttacca attggcgtgc tgagactgtc aacaccgccg ccaacttttg ccagcgccac
    28321 taccgttaat accggtaggc caacggcagt aatgaggaag ccgaatgccg cagtccagac
    28381 gtgttcgcct gcctgcaagc cgaccattgg agggaaaata atgttacctg cgccgacgaa
    28441 caacgcaaat gtcataaagc ccagagcgat gatatcgcgc gatcttaatt gatgggtcat
    28501 aaatcatact gcctgtggat gtggtgttgt aacgttaaat ttttgtgcca atccctgggg
    28561 atgatacagc ggtatattta aacaatttta gcgggaaata ctcccaacca ggcgcggcga
    28621 agaatagttt ttcgatttac cgcgtaaatg cagtcagtat gacagcacct gaagcgcaat
    28681 ttaaacgctt ataacgtttt aaagcaagaa gggatctaaa aaccagaggt atatagcgat
    28741 aagaaatcac caaccagtat aaaacactat ttcaatgaaa aagacatggc taatcatgcg
    28801 aacaaaaaat caccaaccat tgtaaaatct aacgcccgta tttcgactgc aaagaaaata
    28861 agccagcatc ggctggctta tggcaataaa agatgacaaa ggcgaattaa tcgctgtttt
    28921 tggcaattaa acgttccggg ataacaaaac tgaaacgtgt accttttcct actgtactct
    28981 caatattcag gcgactttcg tgatgattca ccgcatgttt cacgatcgct aaccctaatc
    29041 cgctaccacc ggtttgccgg gaacgcgctt tatcaacgcg ataaaaacgc tcggtcaggc
    29101 gcggaatatg ctccggtgca atgcccggtc cgttatcttc aacactaaat tcggcaccgt
    29161 gcggcactcg ctgccagcgt acggtgatat gcgtgccttc cggcgtatga ttcacggcgt
    29221 tatagaccag gttcgaaatc gcactgcgta gctgatcttc attgcctgac accttgaggc
    29281 cgttatctat ctcaaaggta aatgtctgtt ttttctgact cagagtctga gcctcgcgct
    29341 caacgacgcg cagcatcatc ggcacatcaa ccttttcatt gagcaaatgc gtcggtgcgg
    29401 cttctatttt cgacagcgtc agcaattgct tcaccaaccc ttccatccgc tgggtctgct
    29461 cgcgcatggt gtgcaacgct ttttcacgta ccgcgccttc cagcggttgc tcatccatca
    29521 tctccaggta accctgtaac acggtcaatg gcgtacgtaa ctcatggctc acgttggcaa
    29581 aaaagttacg ccgcgcccct tccagttgat gcatttgcgt gacatctcgc gccaccatca
    29641 gcaactgttt gtgggtataa ggcatgacgc gaatctccag atgccgcccg gtgttgagca
    29701 ccagattgag cgggcgagag aaatcacgcg ttttcagata ttgcgtaaac tccgggtaac
    29761 gcagcaagtt aaggatgttc tgcccgttat cttccggcca gcgcaagcca agaatctgtt
    29821 gcgccagacc gttgcaccag aaaataccgc cctcttccgt ggtcagcacc accgcgtcgg
    29881 gcagcgactc cgcgccgcta cgaaagcgtt taatcagatt gcccagttca cggcggcgtt
    29941 ttttatttcg cagctgcatc tggtgtaagc cgtatagtag cggttcccag ctaccacgcc
    30001 ccggtggcgg ggtcatacta cgatccaccc acagccacca tgaaaggcgc aataaattcc
    30061 agaaatgcca gataagcagt cctgttaccg atgccagcaa aaaccagggc aggtaaccaa
    30121 aaaatgcacc caggatgaaa gccgggaggc agcaaagtag cagctccagc accagccttt
    30181 tccacgacag ccgttccagc acgtaagata ctccagttaa gaaatcataa gccctgctct
    30241 acgtccgatg agcaaggcct taaaagcggg ttgaaaaacg ataccctgta ccgcgcacgg
    30301 tctgcaccat gcggtcatgc ccgccgggct ccagtgcttt acgcaggcga cgaatgtgga
    30361 catcgaccgt acgatcttcc acatacacgt tggttcccca gacgtggttt aacagctgct
    30421 cgcggctgta cacgcgctca ggatgcgtca taaaaaagtg cagcagttta aattctgtcg
    30481 gccccatctc cagcggttct tcgcccgcca tcacccggtg agatgtcggg tccagactta
    30541 gcccctgcat ctcgatcacc tcttccaccg ccattggcga aatacggcgc attaccgctt
    30601 tgattcgcgc caccagctcc ttcggagaaa acggcttggt gatatagtca tccgcaccgg
    30661 tttcaaggcc gcgcacgcga tcttcttctt cccctctggc ggtcaacatc accactggga
    30721 tatcccgggt catcgactcg cgcttgaggt gtttgatgaa ctggataccg gagccgccag
    30781 gtaacatcca gtcaaggaga attaaatccg gccagggttc attcagttga ttcacagcac
    30841 tgtcataatc ttccgcttcg accggctgaa agccattttg ttcgagcacg aagcagacca
    30901 tttcgcgaat tggagcttca tcttctacga ccagaatacg tctcgccatg atttgccctg
    30961 ttgtaataga taggttgcga tcattaatgc gacgtcatta tgcgtcagat ttatgacaga
    31021 tttatgaaaa gctcgtcgca catatcttca ggttattgat ttccgtggca cagaaaaaag
    31081 caaatggcac atctgtttgg gtataatcgc gcccatgctt tttcgccagg gaaccgttat
    31141 gcgcatcctt cacacctcag actggcatct cggccagaac ttctacagta aaagccgcga
    31201 agctgaacat caggcttttc ttgactggct gctggagaca gcacaagccc atcaggtgga
    31261 tgcgattatt gttgccggtg atgttttcga taccggctcg ccgcccagtt acgcccgtac
    31321 tttatataac cgttttgttg tcaatttaca gcaaactggc tgtcatctgg tggtactggc
    31381 aggaaaccat gactcggtcg ccacgctgaa tgaatcgcgc gatatcatgg cgttcctcaa
    31441 tactaccgtg gtcgccagcg ccggacatac gccgcaaatc ttgcctcgtc gcgacgggac
    31501 gccaggcgca gtgctgtgtc ccataccttt tttacgcccg cgtgacatta ttaccagcca
    31561 ggcggggctt aacggtattg aaaaacagca gcatttgctg gcggctatta ctgattatta
    31621 tcaacaacac tatgccgatg cctgcaaact gcgcggcgat cagcctctgc ccatcatcgc
    31681 cactggacat ttaacgaccg tgggtgccag taaaagtgac gccgtgcgtg acatttatat
    31741 tggcacgctg gacgcgtttc cggcacaaaa ctttccacca gccgactaca tcgcgctcgg
    31801 gcatattcac cgcgcgcaga ttattggcgg catggaacat gttcgctatt gcggttcccc
    31861 cattccactg agttttgatg aatgcggtaa gagtaaatat gtccatctgg tgacattttc
    31921 aaacggcaaa ttagagagcg tggaaaacct gaacgtaccg gtaacgcaac ccatggcggt
    31981 gctgaaaggc gatctggcgt cgattaccgc acagctggaa cagtggcgcg atatatcaca
    32041 ggagccgcct gtctggctgg atatcgaaat cactactgat gagtatctgc atgatattca
    32101 gcgcaaaatc caggcattaa ccgaatcatt gcctgtcgaa gtattgctgg tacgccggag
    32161 tcgtgaacag cgcgagcgtg tgttagccag ccaacagcgt gaaaccctca gcgaactcag
    32221 cgtcgaagag gtgttcaatc gccgtctggc actggaagaa ctggatgaat cacagcagca
    32281 acgtctgcag catcttttcg ccacgacgtt gcatagcctc gccggagaac acgaagcatg
    32341 aaaattctca gcctgcgcct gaaaaacctg aactcattaa aaggcgaatg gaagattgat
    32401 ttcacccgcg agccgttcgc cagcaacggg ctgtttgcta ttaccggccc aaccggtgcg
    32461 gggaaaacca ccctgctgga cgccatttgt ctggcgctgt atcacgaaac cccgcgcctc
    32521 tctaacgttt cacaatcgca aaatgatctc atgacccacg ataccgccga atgtctggcg
    32581 gaagtggagt ttgaagtcaa aggtgaagcg tatcgcgcgt tctggagcca gaatcgggcg
    32641 cgtaaccagc cggacggtaa tttgcaggtg ccacgcgtag agctggcgcg ctgtgcagac
    32701 ggcaaaattc tcgccgacaa agtgaaagat aagctggaac tgacggcgac gttaaccgga
    32761 ctggattacg ggcgcttcac ccgttcgatg ctgctttcgc aggggcaatt tgctgccttc
    32821 ctgaatgcca aacccaaaga acgcgcagaa ttgctcgagg agttaaccgg cactgaaatc
    32881 tacgggcaaa tctcggcgat ggtttttgag cagcacaaat cggcccgcac agagctggag
    32941 aagctgcaag cgcaggccag cggcgtcgcg ttgctcacgc cagaacaagt gcaatcgctg
    33001 acagcgagtt tgcaggtact tactgacgaa gaaaaacagt taattaccgc gcagcagcaa
    33061 gaacaacaat cgctaaactg gttaacgcgt ctggacgaat tgcagcaaga aggcagccgc
    33121 cgtcaacagg ccttgcaaca ggcgttagcc gaagaagaaa aagcgcaacc tcaactggcg
    33181 gcgctcagtc tggcacaacc ggcacgaaat cttcgtccgc actgggaacg catcgcagaa
    33241 tacagcacgg cgctggcgca tactcgccag cagattgaag aagtaaatac tcgcttacag
    33301 agcacaatgg cgcttcgtgc gagcattcgc caccacgcgg cgaagcagtc agcagaatta
    33361 cagcagcagc aacaaagcct gaatgcctgg ttacaggaac acgaccgctt ccgtcagtgg
    33421 aacaacgaac tggcgggttg gcgtgcgcag ttctcccaac aagccagcga tcgcgagcat
    33481 ctgcggcaat ggcagcaaca gttaactcat gctgagcaaa aacttaatgc gcttgcggcg
    33541 atcacgttga cgttaaccgc cgatgaagtt gctagcgccc tggcgcaaca tgctgagcaa
    33601 cgcccactgc gtcagcgcct ggtcgcactg catgggcaga ttgttcccca acaaaaacgt
    33661 ctggcgcagt tacaggtcgc tatccagaat gtcactctgg aacagacgca acgtaatgcc
    33721 gcacttaacg aaatgcgcca tcgttataaa gaaaaaacgc aacaacttgc cgatgtgaaa
    33781 accatttgcg agcaggaaac tcgcattaaa acgctggaag cccagcgcgc gcagttacaa
    33841 gcgggtcagc cttgtccact ttgtggttcc accagccacc cggcggtcga ggcgtatcag
    33901 gcgctggaac cgggcgttaa tcaggcccgg ctattaacgc tggaaaaaga agtgaaaaag
    33961 ctcggcgaag aaggtgcgac gctacgtggg caactggacg ccttaacaaa gcaacttcag
    34021 cgtgatgaaa acgaagcgca aagcctccga caagatgagc aagcacttac tcaacaatgg
    34081 caagccgtca cggccagcct caatatcacc ttgcagccac aggacgatat tcaaccgtgg
    34141 ctagatgcac aagatgagca cgaacgccag ctgcggttac tcagccaacg gcatgaatta
    34201 caagggcaga ttgccgcgca taatcagcaa attatccagt atcaacagca aattgaacaa
    34261 cgccagcagc agctcttaac ggcattggcg ggttatgcac tgacattgcc acaggaagat
    34321 gaagaagaga gctggttggc gacgcgtcag caagaagcgc agagctggca gcatcgccag
    34381 aacgaattaa ccgcgctgca aaaccgtatt cagcaactga cgccgattct ggaaacgttg
    34441 ccgcaaagtg atgaactccc gcacagcgaa gaaactgtgg tgctggaaaa ctggcggcag
    34501 gttcatgaac aatgtctcgc attacacagc cagcagcaga cgttacagca acaggatgtt
    34561 ctggcggcgc aaagtctgca aaaagcccag gcgcagtttg acaccgcgct acaggcgagc
    34621 gtctttgacg atcagcaggc gttccttgcg gcgctaatgg atgaacaaac actaacgcag
    34681 ctggaacagc tcaagcagaa tctggaaaac cagcgccgtc aggcgcaaac gctggtcact
    34741 cagacagcag aaacgctgac acagcatcag caacaccgac ctggcgggtt atctctcact
    34801 gtgacggtgg agcagattca gcaagagtta gcgcaaactc accaaaagtt gcgtgaaaac
    34861 accacgagtc aaggcgagat tcgccagcag ctgaagcagg atgcagataa ccgtcagcaa
    34921 caacaaacct taatgcagca aattgctcaa atgacgcagc aggttgagga ctggggatat
    34981 ctgaattcgc taataggttc caaagagggc gataaattcc gcaagtttgc ccaagggctg
    35041 acgctggata atttagtcca tctcgctaat cagcaactta cccggctgca cgggcgctat
    35101 ctgttacagc gcaaagccag cgaagcgctg gaagttgagg ttgttgatac ctggcaggct
    35161 gatgcggtac gcgatacccg taccctttcc ggcggcgaaa gtttcctcat cagtctggcg
    35221 ctggcactgg cgctttcaga tctggtcagc cataaaacgc gtattgactc gctgttcctt
    35281 gatgaaggtt ttggcacact ggatagcgaa acgctggata ccgcccttga tgcgctggat
    35341 gccctgaatg ccagtggcaa aaccatcggg gtgattagcc acgttgaggc gatgaaagag
    35401 cgtattccgg tgcagatcaa agtgaagaag atcaatggac tgggctacag caaactggaa
    35461 agtgcgtttg caatgaaata actattcagc aggataatga atacagaggg gtgaattatc
    35521 tcttggcctc gctggtcgtt atcctgcaat ctatcacttt attggctacg gtgattggta
    35581 gccgttctgg tggttgtgat ggtggtatga aaaaagttat tttatctttg gctctgggca
    35641 cgtttggttt ggggatggcc gaatttggca ttatgggcgt gctcacggag ctggcgcata
    35701 acgtaggaat ttcgattcct gccgccgggc atatgatctc gtattatgcg ctgggggtgg
    35761 tggtcggtgc gcctataatc gcgctttttt ccagccgcta ctcactcaaa catatcttgt
    35821 tgtttctggt ggcgttgtgc gtcattggca acgccatgtt cacgctctct tcgtcttacc
    35881 tgatgctcgc cattggtcgg ctggtttccg gctttccgca tggtgcattt tttggcgtcg
    35941 gcgcgatcgt gttatcaaaa attatcaaac ccggcaaagt caccgccgcc gtggcaggga
    36001 tggtttccgg gatgacagtc gccaatttgc tgggcattcc actgggaacg tatttaagtc
    36061 aggaatttag ttggcgttac acctttttgt tgatcgctgt ttttaatatt gcggtgatgg
    36121 catcggtcta tttttgggtg ccggatattc gcgacgaggc gaaaggaaaa ctacgcgaac
    36181 aatttcactt tttacgcagc cctgccccgt ggttaatttt cgccgctacg atgtttggca
    36241 acgcaggtgt gtttgcctgg ttcagctacg taaagccata catgatgttt atttccggtt
    36301 tttcggaaac ggcgatgacc tttattatga tgttagttgg gctagggatg gtgctgggga
    36361 atatgctaag tggcaggatt tcaggacgtt attcaccact gcgcattgca gcagtgactg
    36421 actttatcat tgtactggca ctgctgatgc tctttttctt cggcggtatg aaaacaacgt
    36481 cgcttatttt tgcttttatt tgttgcgcgg gattatttgc cctatcagca cctctacaaa
    36541 tattattact gcaaaatgcc aaaggcggag agttattagg tgccgcaggt gggcaaatag
    36601 cgtttaacct cggtagcgcc gtcggcgcat attgcggtgg tatgatgctg acgctggggc
    36661 tggcatataa ttacgtggcg ctgcccgctg ccctgctttc gtttgctgcg atgtcgtcgt
    36721 tgctgctgta tggtcgctat aagcgccagc aagcggcgga tagttcggtg ctggcgaaac
    36781 cactggggta gatagcagtc gcagtgcgca tcgcttgttg ccggatgcgg cgtgaacgcc
    36841 ttatccggcc tacgggactg ttcattttgt aggcctgata agacacgcca gtgtcgcatc
    36901 aggcatcgga gcaccggggg ttgccgccac tgtgcgcgtt atttgttgcc ggatgcggcg
    36961 cgagcgcctt atccggccta ctggacggct cactttgtag gcctgataag acgcggcaag
    37021 cgtcgcatca ggcatgatac gccaattgcc tggttttttt actcttgtgg ccataaccat
    37081 gcagcgccgc gtacgccgct cgaatcacca tgtttcgcct tgcgtaccgg cgtttcacat
    37141 tctccgccga agacaaattg tttaatcaac tgtggaaccg tttgatataa acggtctaca
    37201 ttgctcattc cgccgcccag gacaatcaca tccggatcga gaatattcac gacatgtgcc
    37261 agcgattttg ccagccgcag ctcgtagcga cgcaatgcca gttccgctac cggatcgctt
    37321 tcttctacca ggcggataat ttcactgcct ttcagcgcat gtccgctcaa acgacgataa
    37381 tccgtcgcga atcccgtgcc tgaaataaag gtttcaatac aaccttgttt accgcaataa
    37441 caagggactt cctcgcgata acgcagttcg tcttcgtcca tccacggtag cggattgtgt
    37501 ccccactcgc ctgccgtgcc attgccgccg atatgcgccc gcccattgaa tgccacgccc
    37561 gcgccgcatc ccgtgccgat aatcacggca aaaaccgtct gcgctcccgc tgccgcgcca
    37621 tctaccgctt ctgaaaccgc cagacagtta gcgtcatttg ccagccgcac ttcccgctgc
    37681 aaccgcgcgc ttaagtcttt atcgaatggc tgaccgttga gccaggttga attggcattc
    37741 ttcaccacgc cggtgtaggg cgaaatcgaa ccaggaatgc ccatacctac cgttccgcgc
    37801 tgccctgttg cctgctccgc catatcaacc aacgtggcga tcgtttcaat agtctgccgg
    37861 taatcatcac gcggcgtggg cagacgatgg cggtacaact gctcccctgc atcgcccaat
    37921 gcaatcactt cagttttggt gccgcctaaa tcgataccta tacgcacggt actctcctta
    37981 tttttttcaa tatcaatagc gtagagacgg acaaccggat tggcaatgca aggccgccga
    38041 caattcgtta tcatgcccgc taaatttaac gacaaggccg tggaaattat catgctgtgg
    38101 ttcaaaaatt taatggttta ccgtcttagc cgcgagattt cgctgcgtgc agaagagatg
    38161 gaaaaacagc tagcctcgat ggcatttacc ccatgcggca gccaggacat ggcgaagatg
    38221 ggctgggtac ctccgatggg atcgcacagc gatgcgttaa cgcacgttgc caatggtcaa
    38281 attgttatct gtgcgcgcaa agaagaaaaa atcctcccgt ccccggtgat taaacaggcg
    38341 ctggaagcga aaatcgccaa actggaagcg gaacaggcgc gtaagctgaa gaaaaccgaa
    38401 aaagattcgc tgaaagacga agtgctgcac tctctgctgc cgcgtgcttt cagccgtttt
    38461 agccagacaa tgatgtggat cgacacggtt aacggtttga ttatggtgga ctgcgccagc
    38521 gccaaaaaag cggaagatac gctggcactg ctgcgtaaaa gcctggggtc gttaccggtt
    38581 gtaccattga gcatggaaaa cccgattgaa ctgacgctga ccgaatgggt acgttccggc
    38641 agcacggcac agggcttcca gctgcttgat gaagccgagc tgaaatcgtt gctggaagat
    38701 ggcggcgtga tccgcgcgaa gaaacaagat ctgaccagcg aagagatcac caaccacatt
    38761 gaagccggaa aagtggtgac taaactggcg cttgactggc agcagcgcat tcagtttgtg
    38821 atgtgcgacg atggttcgct caagcgtctg aagttctgcg acgagctgcg cgatcaaaac
    38881 gaagatatcg accgtgaaga tttcgcccag cgctttgatg ccgatttcat cctgatgact
    38941 ggtgaactgg cagcgttaat tcaaaacctg attgaaggat taggtggcga agcacaacgt
    39001 taattgctga tttaccttta atgccggatg cgacgcctgc cgcgtcttat ccggcgtacg
    39061 aagccacatc aggaatataa tcgttagcta cagcgggcaa taattcttag cgcagcaata
    39121 ttatgcgttt tatgctgtaa cttgctccag gggcgttgtg tcattgtttt tcctcaagcc
    39181 ggtgacgcgc ttcactacga atttttcgcg ttgccccggt tttcactaaa tcacgtaaca
    39241 gcgaaagatc gggttgttcg acaaataaga gtaatgatgg atcctctttt aaccacgccg
    39301 ttttcacatc cgcagccagc tgtgaattat taatagccag cgaccgctcc tgaggctcag
    39361 tcaatgtcgt taacaacaac accggagtat gtgcgttgtg caatgccgtt tggcgcaaat
    39421 gatcattgcg actttgcagc aatgcacacc accattcatc atctgattta tcttttaacg
    39481 tcgccagttc cgcaacaata tctggagatc ttcgctgata tttatatcaa ccttgatgaa
    39541 ttatgaatat atcggaaatc gaaaaaatcc cagcaaagga aatattatta tgggttaaag
    39601 agaggacatt tcagaaaaat gccctcccgt tcaaggggaa ggcgaggaat tacagatagc
    39661 ggcacagata agaggtgggg tcggcaactt gcagatgaaa ctcgctgtga ccgggaacat
    39721 taaacaccga accggcttca tacacttgcc agtcggtcgc gtcaggcagt aacacattca
    39781 gcgcgccact gattaccgtc atctcttcag gctcagcggt gctgaaggtg tattcacctt
    39841 caaccataac acccacgctg gcgcgaccag tgctgctgct ggaaaagccg atcgatttca
    39901 ctttgccgga aaagtactca ttactttgaa gcataaactg gccctgtctg gtagttgaac
    39961 atctacttca ctataggggc cagaggcgct ggctgtcacg caaaattaca tcagtaattc
    40021 cgcagccagc cgtgcaatca atacgttaga gagcaagaca ggaacatcga gctgtttttg
    40081 cagtaaatcg cgatgacgct ggttaaatcc caaacaatcc agcatgatga catctgcacc
    40141 ttttgccagt aattctttcc cggcatcaat gattttttgt tccgaatcat gaatggggtt
    40201 acccaatgaa aatactggcg gtttctgcaa aatttgccat ttttgcgcct gaacggtcag
    40261 caactcctca accggtacga taacccccac ctgatgatct tcaacaatag aggaaaccag
    40321 cggtggcaat atccgcgacg gctcaagaaa gatcgtatta cgcgcggtca tactactaat
    40381 gtttgctgta ctcattaata taatgacgtc gtaaccctga ttatcgagca cttcaaccac
    40441 accttgcagg tctcgctcca ctttgcgacg cgaaacatgg gccagatggt tgtcatttaa
    40501 taatgtgaga attgtgtctt cgcctgcttc tggcgcgtac tccgccatca cttcttcgcg
    40561 gcttaacttc cccagcaggc tatgatggga aatattatct tcgtcaatgt attctgtcag
    40621 gagcggcaaa acttcctgca taggtacaat gccgatagtc aggatcgcca acgacgcact
    40681 cattgttctt ccaccttact tttatttcgt cgttaattac cgggcaagtg ttaagcacca
    40741 ctgctgacac ttacttgtgc tacaaatgac aaaaagcgta gcagcagacc tgcactgcat
    40801 aatgtaaaga ttccaaatga ttccagtaat ggattgttat tgtttaatat tctaattatt
    40861 tgaaaaacat gaattgtgaa aaatgtgacg cagatccctt gtggcggcac tcagaatccc
    40921 ttccgagcag tctgatgcgg tcactctttg tcagggtcct catagcgttt tttcgcatcc
    40981 atcgcttcct gagcggtcgg atgttcactg atcaacgagt ctggtttagg atgatcggct
    41041 ctgagttggt accaggttac cgtctgtcct ggctttcctt tttcaatcgt cactatatat
    41101 gcttcacgag gataaggcgg tttcgttggc atcgttcttc cttatttcac gggatgaacg
    41161 ttaagtatag gcgctcgaaa atcaacaatt gatcgtctgt gccagagcgc tgcgaatttc
    41221 agaaatcacc tggctgggtt cgtttgttgc gtcgatgata atatgcgcaa cttcgcgata
    41281 tagcgcatcg cgttcttcca gcacttcctg aacttcttcg ctcagtggtt ttcccgttaa
    41341 ggttggccgt aaatcttctt ccggtgcagc ttgcagtcgg ttaaccagga ctgatactgg
    41401 cgcacacaaa taaaccacga tcccgttatt ttgcatgaag tgacgattaa atttcgtcag
    41461 aataatgccg ccgcctgtag cgataacggt ggatggcgca gttaccgctt ccagcgccgc
    41521 cgtttctctg gcgcggaatc ccgcccactc ttccctttcg acgatctccg cgaccgtcat
    41581 attgagctgt gattgcaacc acaggtcggt atcgacaaac cgacggttaa gcgaatcggc
    41641 aagggccatt ccgaccgttg ttttaccaca gccccgaggc ccgatcagaa aaagaggttg
    41701 tgtcatcgtg ggttttcccc aataggtcgc aatgcggcga aagccggtgt catgagaata
    41761 gcgatcatac catcaaacta gtacaatttc gattgtaaag aaaaaattcc acttaaggtg
    41821 gaaatctcaa tacatccctt actataccaa taaatattca agaatgaagt gtaaataata
    41881 aattacattt agccacgact acgttgcact tccagccacc acttctccag ctccgcagca
    41941 aaagcctggc ggtcacgttg tgaaagacta tctggtcctc cggtctggat cccactggca
    42001 cgtaaggtat ccataaaatc gcgcatcgtc aggcgctcac gaatggtcgc tggcgtgtaa
    42061 cgttcgccgc gcggattaag cgccgccgcg cctttctcga tggcttcagc agccaaaggt
    42121 atatctgcgg tgatcaccaa atcgcccgct tcacactgcc gaacaatttc gttatcagca
    42181 acgtcgaaac ctgccgcgac gcgcagcgta cgaataaatc gcgatggcgg cacgcgtaaa
    42241 ctctggtttg ctaccagtac cagcggcatc tgcatacgtt ccgccgcgcg atacaaaata
    42301 tctttaatta cattgggaca cgcgtcggca tccacccaaa ttgtcataaa gtcatccttt
    42361 gttgggtaat cctctattgt gtcgcgcttt tgccttccgg catagttctg tttatgcttc
    42421 tgccagcgat tatcaaaaca atgaatttca cggcaggagt gaggcaatgg aaaagaaaat
    42481 cggttttatt ggctgcggca atatgggaaa agccattctc ggcggtctga ttgccagcgg
    42541 tcaggtgctt ccagggcaaa tctgggtata taccccctcc ccggataaag tcaccgccct
    42601 gcatgaccag ttcggcatca acgccgcaga atcggcgcaa gaagtagcgc aaatcgccga
    42661 catcattttt gccgccgtta aaccaggcat catgattaaa gtgcttagcg aaatcacctc
    42721 cagcctgaat aaagactctc tggtcgtttc tattgctgca ggtatcacgc tcgaccagct
    42781 tgcccgcgcg ctgggccatg accggaaaat tatccgcgcc atgccgaaca ctcccgcgct
    42841 ggttaatgcc gggatgacct ccgtaacgcc aaacgcgctg gtaaccccag aagataccgc
    42901 tgatgtgctg aatattttcc gctgctttgg cgaagcggaa gtgattgctg agccgatgat
    42961 ccacccggtg gtcggtgtga gcggttcttc gccagcctac gtctttatgt ttatcgaagc
    43021 gatggccgac gccgccgtgc tgggcgggat gccacgcgcc caggcgtata aatttgccgc
    43081 tcaggcggta atgggctccg caaaaatggt gctggaaacg ggagaacatc cgggaacact
    43141 gaaagatatg gtctgctcac cgggaggcac caccattgag gcggtacgcg tactggaaga
    43201 gaaaggcttc cgtgccgcag tgatcgaagc gatgacgaag tgtatggaaa aatcagaaaa
    43261 actcagcaaa tcctgatggc ttttgccgga cgtcaggccg ccacttcggt gcggttacgt
    43321 ccggctttct ttgccttgta gagcgccaaa tctgccgatt tcaaccactc gcgatagtga
    43381 ctcatttgtg gattcagcgg cgcaaccccc acactaatcc gtaaagttac ctgtggcgta
    43441 ttcggcaagc gtaacgtgtt tagcccttca tgcacccgta acatggcggt gatggcgctc
    43501 tctgctggcg taccggacat gattactgca aactcatcgc cgccaaaccg accaatcaca
    43561 tcgctaccgc gtagggtaat ttgtaactgt cgggtaagcg ccacaatcgc ttcatcaccc
    43621 acatcatggc cccaggtatc gttgatgctc ttgaaatggt cgatatcgat aatcagtaac
    43681 gttgcatcgc gattatgccg ccgacagtta tcaaattcat tgcgtaacat agtttcccaa
    43741 tgacgtcggt tatacacgcc cgtcataccg tcgcgggtac tcatgacctg caacctgcgt
    43801 ttatgttccg ccagtttagt cgccgtctgg tagctgaccc agccaaacag cagaggataa
    43861 atgacaataa tgggaaggga gagccaccat tccagcggtg cgctattgaa cgacacggtg
    43921 atgcccgtca gctcgagggt gacaaggcag gagaccacca tcaacaccag acccgcgaca
    43981 aacagacggg ggccgcctgc ccccatcaaa ttcagacaca taatcatcaa catcgcggtg
    44041 gaaggcagtg cgtttacgcc cattacgcct acccacattc ccgctaatac tgcatcggtt
    44101 tttaagttgt aaatttcccg gctaagcgga tcgacggccc tgctcgctat ttgccaggct
    44161 aaatgcggcc agacgaacgc ccagccgacc aacaacagcc accaccagcc cggcgacgga
    44221 tgcgaaacca gcgttgaagc aatcggtaag aacatgccag ccaggccaac cgcgcggggt
    44281 agtctgacgc gacgggcaaa gcgcagcccg gaccgctgat gttcgttttg aggagttaaa
    44341 ggaggttcct ccccatgcgc cgccgctttt ttgaaaaagt tttcatcatt cattattttt
    44401 gggaacattc agaaacaatt ttcccaaatt atagagacgg ggctaaaggc agaagtatga
    44461 aatttcgggt gagccatttt cgtagctcac ccgttagcac tatcaggtat caggcggctt
    44521 tcttgaggca ggcactcata aacttattac gatcatcccc tttcagagat tgttgtgttg
    44581 cttgattatt acattcgcgc atcttttgct gctgtggcgt caaacttttt tcaccaggcg
    44641 cagatttgct gttcttcagg caatcactca tgtaggtctt acgagcatcc cctttcaacg
    44701 cctgcgccgt cgcctgctga ttacaagagg tcatacgctg ttgttgtggg gttaaggttc
    44761 tctcggcagc gccgacggtg gttaaaaaaa ccagaccgaa aagcaaggta accagtaatg
    44821 taattttcat cgcaccatcc ctcttcatgt tttaaccatg agcgtatgcg cccgtgatct
    44881 gtcattaagt ctggttgcta acagcaaaaa aaccacccgg cagcgaaaat tcactgccgg
    44941 gcgcggtttt atttcagccc cagggcggct ttcatggtgt agaagagatc ggtctggtcg
    45001 gtcagtccaa caacattggc ggcatgcggg ccatacgccg caatacgcaa ctgactgccg
    45061 gtatgttctt gtgaatcctc ttcggagttc ccgtaactca tcaccatcac tgcgccatct
    45121 ttggtattta gcgcctgggt gagccccgga gctttggtat ccggcgcaac tatctggctg
    45181 gcatgggcgt gatcagcggt gactatgacc aacgtgttac catccttttt agcgaattcc
    45241 agcgcccgtt gtacggcttc atcgagatcg accgtctcgc caatttgccc acaaggattc
    45301 gcagcgtgat cctgtttatc gattgacgca ccttcaactt gcaggaaaaa gcctttctca
    45361 tttttactca acaattcaat ggctttgtcg gtcatctgcg ccagggtcgg tacactgtca
    45421 ttacgttgcg gattaggcgt acaggtgact gcgggcttat ctatattacc gtggtacgtt
    45481 gctttcggtc cttgccagcg cactggcata ttgccgtcag caaacagtcc taataggggt
    45541 ttttgctgat tcgcttccgt caccgaattc agtgaggcag catcgctcac caactgataa
    45601 ccacgcgcct gtgcctgttc acgcagcgtt tttccctgcc attcaccggc ggttgccgtt
    45661 tcagcaaagg tttttgcacc gccgccaagc gtaacatcgg cacgggcgtt aagcagctgt
    45721 tcggtaatcg atccttttcc gcctttttcc agagcgttac ccggacattt ttcactggtc
    45781 gcgctcggac cgtagcattt gcgcgaggtc acatgcgcca ccagcgcagc gggcgtggca
    45841 tcctgcaact ctgcggtaga aacgttaccg gtcgccagac ctgcggcttt tgccatttcc
    45901 agaatcgttg ggtgatcttt ttcgtgaata tcgacgccca gcgcgccatt ataggttttg
    45961 acacctgttg accaggcggt tgctgatgca gccgagtcgg tgacgtagtc cggtttgccg
    46021 gtttttttat tcagcgcata gtgagtgtat tgcccggtaa gcggtaaggc atcgatacct
    46081 ttaaaaaagc cgcccgcacc ttcggcataa ttgcgtgcgg cagtaatttc cgagtccccc
    46141 atcccatcgc caatcagcaa aataatattt tttgccggtt tatcgctaag agaatcacgc
    46201 agagcggcgg tctgatcacc cgttaagcgg cgagcaccgc cgggtgcagt aatatcgccc
    46261 tgagctgccc ggttttccag aacaggcatt tctggtgtcc gggcttttgt cacaggggta
    46321 aacagtaacg gtaagagtgc cagtgcaata gtgctttgtt tcactttatt ttctccatgt
    46381 acaaatacat taaaaaataa aaacaaagcg actataagtc tcggccgtga caactttatg
    46441 acagctgttg aaaagattaa ctttttactg acgaggatgc ctcaataact tctttacgta
    46501 atcgcgcagc agctccgtat cgtcgtcagg aatgctggca tcgggcttta cctcgtacag
    46561 cgccccctct acctgatcaa tcaaccgctg ttgatcattt tgcgccatat tgcgaagcat
    46621 tgccgtgacg ataatctcca aagcttctac ctgcgcgcac agttctttcg actcttcttc
    46681 tttttgggca agcttaaata acaactcagc aatgagattt ttcatgtctg tatttcctta
    46741 tccaaagtat ggagaagtta acattatgtt tcacaacttc atagtgtctg gaagtattat
    46801 ttttatgaca aaggaaatac tttgcgcaaa tattttttgt ttga
//
LOCUS       DADSYM010000012        46579 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2415
            SAMN08039579-rid11723173.denovo.012, whole genome shotgun sequence.
ACCESSION   DADSYM010000012 DADSYM010000000
VERSION     DADSYM010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039579
            Sequence Read Archive: SRR6362533
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 46579)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 46579)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980162.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 03:39:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,484
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 4,347
            CDSs (with protein)               :: 4,347
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 43 of 85
            Pseudo Genes (incomplete)         :: 37 of 85
            Pseudo Genes (internal stop)      :: 19 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..46579
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039579-rid11723173.denovo.012"
                     /strain="LGE2415"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8302 N 95.9628 W"
                     /collection_date="2015-08-24"
     gene            87..362
                     /locus_tag="J1Y83_000584"
     CDS             87..362
                     /locus_tag="J1Y83_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA7628769.1"
                     /translation="MPTVLSRMAMQLKKTAWIIPVFMVSGCSLSPAIPVIGAYYPGWF
                     FCAIASLILTLITRRIIQRTNINLAFVGIIYTALFALYAMLFWLAFF"
     gene            381..1412
                     /gene="mdtN"
                     /locus_tag="J1Y83_000585"
     CDS             381..1412
                     /gene="mdtN"
                     /locus_tag="J1Y83_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter periplasmic adaptor
                     subunit MdtN"
                     /protein_id="HBA7628770.1"
                     /translation="MESTPKKAPRSKFPALLVVALALVALVFVIWRVDSAPSTNDAYA
                     SADTIDVVPEVSGRIVELAVTDNQAVKQGDLLFRIDPRPYEANLAKAEASLAALDKQI
                     MLTQRSVDAQQFGADSVNATVEKARAAAKQASDTLRRTEPLLREGFVSAEEVDRARTA
                     QRAAEADLNAVLLQAQSAASSVSGVDALVAQRAAVEADIALTKLHLEMATVRAPFDGR
                     VISLKTSVGQFASAMRPIFTLIDTRHWYVIANFRETDLKNIRSGTLATIRLMSDSGKT
                     FEGKVDSIGYGVLPDDGGLVLGGLPKVSRSINWVRVAQRFPVKIMVDKPDPEMFRIGA
                     SAVANLEPQ"
     gene            1412..3463
                     /gene="mdtO"
                     /locus_tag="J1Y83_000586"
     CDS             1412..3463
                     /gene="mdtO"
                     /locus_tag="J1Y83_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313090.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter permease subunit
                     MdtO"
                     /protein_id="HBA7628771.1"
                     /translation="MSALNSLPLPVVRLLAFFHEELSERRPGRVPQTMQLWVGCLLVI
                     LISMTFEIPFVALSLAVLFYGIQSNAFYTKFVAILFVVATVLEIGSLFLIYKWSYGEP
                     LIRLIISGPILMGCMFLMRTHRLGLVFFAVAIVAIYGQTFPAMLDYPEVVVRLTLWCI
                     VVGLYPTLLMTLIGVLWFPSRAITQMHQALNDRLDDAISHLTDSLAPLPETRIEREAL
                     ALQKLNVFCLADDANWRTQSAWWQSCVATVTYIYSTLNRYDPTSFADSQAIIEFRQKL
                     VSEINKLQHTIAEGQCWQSDWRLSEDEAVAARECNLENICQTLLQLGQMDPNTPPTPA
                     AKPPSMVADAFTNPDYMRYAVKTLLACLICYTFYSGVDWEGIHTCMLTCVIVANPNVG
                     SSYQKMVLRFGGAFCGAILALLFTLLVMPWLDNIVELLFVLAPIFLLGAWIATSSERS
                     SYIGTQMVVTFALATLENVFGPVYDLVEIRDRALGIIIGTVVSAVIYTFVWPESEART
                     LPQKLAGALGMLSKVMRIPRQQEVTALRTYLQIRIGLHAAFNACEEMCQRVVLERQLD
                     SEERALLIERSQTVIRQGRDILHAWDATWNSAQALDNALQPDRAGQFADALEKYAAGL
                     ATALSRSPQITLEETPASQAILPTLLKQEQHVCQLFARLPDWTAPALTPATEQAQGAT
                     Q"
     gene            3460..4926
                     /gene="mdtP"
                     /locus_tag="J1Y83_000587"
     CDS             3460..4926
                     /gene="mdtP"
                     /locus_tag="J1Y83_000587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter outer membrane
                     subunit MdtP"
                     /protein_id="HBA7628772.1"
                     /translation="MINRQLSRLLLCSILGSTTLISGCALVRKDSAPHQQLKPEQIKL
                     ADDIHLASSGWPQAQWWKQFNDPQLDALIQRTLSGSHPLAEAKLREEKAQSQADLLDA
                     GSQLQVAALGMLNRQRVSANGFLSPYAMDAPALGMDGPYYTEATVGLFAGLDLDLWGV
                     HRSAVAAAIGAHNAALAETAAVELSLTTGVAQLYYSMQASYQMLDLLEQTRDVIDYAV
                     KAHQSKVAHGLEAQVPFHGARAQILAVDKQIAAVKGQITETRESLRALIGAGASDMPE
                     IKPVALPRVQTGIPATLSYELLARRPDLQAMRWYVQASLDQVDSARALFYPSFDIKAF
                     FGLDSIHLDTLFKKTSRQFNFIPGLKLPLFDGGRLNANLEGTRAASNMMIERYNQSVL
                     NAVRDVAVNGTRLQTLNDEREMQAERVEATCFTQRAAEAAYQRGLTSRLQATEARLPV
                     LAEEMSLLMLDSRRVIQSIQLMKSLGGGYQTAPVVEKK"
     gene            5124..7271
                     /gene="fdhF"
                     /locus_tag="J1Y83_000588"
     CDS             5124..7271
                     /gene="fdhF"
                     /locus_tag="J1Y83_000588"
                     /EC_number="1.17.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:5541..5543,aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase subunit alpha"
                     /protein_id="HBA7628773.1"
                     /translation="MKKVVTVCPYCASGCKINLVVDNGKIVRAEAAQGKTNQGTLCLK
                     GYYGWDFINDTQILTPRLKTPMIRRQRGGKLEPVSWDEALNYVAERLSAIKEKYGPDA
                     IQTTGSSRGTGNETNYVMQKFARAVIGTNNVDCCARVUHGPSVAGLHQSVGNGAMSNA
                     INEIDNTDLVFVFGYNPADSHPIVANHVINAKRNGAKIIVCDPRKIETARIADMHIAL
                     KNGSNIALLNAMGHVIIEENLYDKAFVASRTEGFEEYRKIVEGYTPESVEDITGVSAS
                     EIRQAARMYAQAENAAILWGMGVTQFYQGVETVRSLTSLAMLTGNLGKPHAGVNPVRG
                     QNNVQGACDMGALPDTYPGYQYVKDPANREKFAKAWGVESLPAHTGYRISELPHRVAH
                     GEVRAAYIMGEDPLQTDAELSAVRKAFEDLELVIVQDIFMTKTASAADVILPSTSWGE
                     HEGVFTAADRGFQRFFKAVEPKWDLKTDWQIISEIATRMGYPMHYNNTQEIWDELRHL
                     CPDFYGATYEKMGELGFIQWPCRDTSDADQGTSYLFKEKFDTPNGLAQFFTCDWVAPI
                     DKLTDEYPMVLSTVREVGHYSCRSMTGNCAALAALADEPGYAQINTEDAKRLGIEDEA
                     LVWVHSRKGKIITRAQVSDRPNKGAIYMTYQWWIGACNELVTENLSPITKTPEYKYCA
                     VRVEPIADQRAAEQYVIDEYNKLKTRLREAALA"
     gene            7646..9214
                     /locus_tag="J1Y83_000589"
     CDS             7646..9214
                     /locus_tag="J1Y83_000589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005225713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBA7628774.1"
                     /translation="MIKKLLPLLVLSTISATTVAATPPNTLVVAQGLDDIVSLDPAEA
                     NELSSIQTVPSLYQRVVQPDRNDPTKITPILAESWQADPAAKTLTIKLKSDAKFASGN
                     PVRPEDVIYSYVRAVTLNKSGAFILNVLGWQPENIASQLKKIDDHTVQVQWSADVSSA
                     LALNILSTPIASIVDEKLVAPNAKNNDFGNEWLKMHSAGSGAFKMRTYQPHQAIVMEA
                     NASSPTGAPKLKNVIIKNVPDPATRRLLIQQGDADMARDLGADQIDALQGKPGVKVMS
                     IASAEQNYLAFNTGNKDNPLMSNPAFWEAARWLVDYDGITKNLLKGQYFTHQSFLPVG
                     FPGALEETPFTFNPAKAKEILAKAGIKDPHFTLDVENKPPFITIAQSIQASFAQGGVK
                     VDLLPAAGSQVYSRVRAHQHQGAIRMWLPDYFDAHSNASSFAYNDGKSSTVAGLNGWK
                     IPELNKETLAAIAEPDSTKRLDLYKKMQQELQRSSPYVFIDQGKTQIVMRDNVQGYQQ
                     GLNADMVWFDQVTK"
     gene            9314..10363
                     /locus_tag="J1Y83_000590"
     CDS             9314..10363
                     /locus_tag="J1Y83_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012579036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBA7628775.1"
                     /translation="MSVVFSTRARWRGKRPLLALLQGLFTVALTLLGLLLITFALSAL
                     SPVDRVLQIVGDHASQSTYDQVRHQLGLDQPLAVQFWHYLMNLAHGDLGIASSTGQPV
                     LQDLLSAFPATIELATLALIVGAVVGVIAGVLCARYVGSPLDFTVRTLTLLGNSVPVF
                     WLGLLMLALFYARLQWSAGPGRLDDIYQFTIEPRSGFALIDTWLSGDREAFTNAINHL
                     ILPVLLLAYFSLASITRLTRSACLGEMNKEYILLARAKGASEMTILLRHVLPNIRGTL
                     LTVIALAYTSMLEGAVLTETVFSWPGIGRYLTTALFAGDTTAIMGGTLLIGVCFVLIN
                     NLTDLLVRLTDPRLR"
     gene            10363..11196
                     /locus_tag="J1Y83_000591"
     CDS             10363..11196
                     /locus_tag="J1Y83_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005225711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBA7628776.1"
                     /translation="MPMFLFLRRLRHSPAAFCGLIIILLLMLIALFAPWLAPHDPNWQ
                     DAAARLQAPSRQHWLGTDSYGRDLLSRLIYGSRPALGLVALVTIITLPVGLLIGILAG
                     YYGGWLERVLMRFTDVVMSMPRLILAFAFVAMLGPGLVNGALALALTTWPAYARQARS
                     EIQRLRHSDYLAAAEMMGIRGRRLLAGHILPLCLPSAIVRLALDLAGIILAAAGLGFL
                     GLGARPPMAEWGAMIADGMQVIFDQWWIAAAPGAIILIASLAFNLLGDGLRDVLEPQH
                     D"
     gene            11189..12025
                     /locus_tag="J1Y83_000592"
     CDS             11189..12025
                     /locus_tag="J1Y83_000592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001517582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBA7628777.1"
                     /translation="MTDSRLSINNLCVDYPESRVVNNVSFTLGNERLALVGESGSGKS
                     MTARALMGLVRKPGVVTADTLNILGRDALTLNARGWQQLRGNDIAMVLQDPRYALNPV
                     KTVKAQLEEALTLHQRLNRREKEEKINTAIQAVGLNTRVLQSYPRELSGGMGQRVMIA
                     IALINDPRVLIADEPTSALDVRLRNQILELLVTQCEQRQMAMLLISHDLPLVAQFCHR
                     VLVMYQGNKVDEMHAAALPTATHPYTRTLWTCRPNAQTYGQMLPTLDRTAMTPEKYHD
                     DC"
     gene            12012..12716
                     /locus_tag="J1Y83_000593"
     CDS             12012..12716
                     /locus_tag="J1Y83_000593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016238218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBA7628778.1"
                     /translation="MTIVEINHLQVTFAEKTAVSAASFSVNAGETFSLIGESGCGKST
                     ILRVIAGLQRDWHGQVSLFGHTIIPGMRFQGDLRRNVQMVFQDPYASLHPNHTLWRTL
                     AEPLKIRGEREIEKRVQTALEQVGLPFDTARRYPHQLSGGQRQRVVIARALLLRPQLL
                     LLDEPTSALDMSVQAEILNLLNQLKLEHQMTYLLVSHDADVIAHMSDRAALMSEGKIQ
                     RFFDRDAMEKGEHRMD"
     gene            12827..13516
                     /locus_tag="J1Y83_000594"
     CDS             12827..13516
                     /locus_tag="J1Y83_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sel1 repeat family protein"
                     /protein_id="HBA7628779.1"
                     /translation="MKKIITLMLFLTFFAHANDSEPGSQYLKAAEAGDRRAQYFLADS
                     WFSSGDLSKAEYWAQKAADSGDADACALLAQIKITNPVSLDYPQAKVLAEKAAQAGSK
                     EGEVTLAHILVNTQAGKPDYPKAISLLENASEDLENDSAVDAQMLLGLIYANGVGIKA
                     DDDKATWYFKRSSAISRTGYSEYWAGMMFLNGEEGFIEKNKQKALHWLNLSCMEGFDT
                     GCEEFEKLTNG"
     gene            complement(13664..14977)
                     /gene="gltP"
                     /locus_tag="J1Y83_000595"
     CDS             complement(13664..14977)
                     /gene="gltP"
                     /locus_tag="J1Y83_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate:proton symporter GltP"
                     /protein_id="HBA7628780.1"
                     /translation="MKNIKFSLAWQILFAMVLGILLGSYLHYHSDSRDWLVVNLLSPA
                     GDIFIHLIKMIVVPIVISTLVVGIAGVGDAKQLGRIGAKTIIYFEVITTVAIILGITL
                     ANVFQPGAGVDMSQLATVDISKYQSTTEAVQSSSHGIMGTILSLVPTNIVASMAKGEM
                     LPIIFFSVLFGLGLSSLPATHREPLVTVFRSISETMFKVTHMVMRYAPVGVFALIAVT
                     VANFGFSSLWPLAKLVLLVHFAILFFALVVLGIVARLCGLSVWILIRILKDELILAYS
                     TASSESVLPRIIEKMEAYGAPASITSFVVPTGYSFNLDGSTLYQSIAAIFIAQLYGID
                     LSIWQEIILVLTLMVTSKGIAGVPGVSFVVLLATLGSVGIPLEGLAFIAGVDRILDMA
                     RTALNVVGNALAVLVIAKWEHKFDRKKALAYEREVLGKFDKTADQ"
     gene            complement(15320..15916)
                     /gene="nrfG"
                     /locus_tag="J1Y83_000596"
     CDS             complement(15320..15916)
                     /gene="nrfG"
                     /locus_tag="J1Y83_000596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase NrfEFG subunit NrfG"
                     /protein_id="HBA7628781.1"
                     /translation="MKQPQIPVKMLTTLTIFMVFLCVGSYLLSPKWQAVRAEYQRQRD
                     PLHQFASQQTPEAQLQALQDKIRANPQNSEQWALLGEYYLWQNDYSNSLLAYRQALQL
                     RGENAELYAALATVLYYQASQHMTVQARAMIDKALALDSNEITALMLLASDAFMQANY
                     AQAIELWQKVMDLNSPRINRTQLVESINMAKLLQRRSD"
     gene            complement(15913..16296)
                     /gene="nrfF"
                     /locus_tag="J1Y83_000597"
     CDS             complement(15913..16296)
                     /gene="nrfF"
                     /locus_tag="J1Y83_000597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase NrfEFG subunit NrfF"
                     /protein_id="HBA7628782.1"
                     /translation="MNKGLLTLLLLFTCFARAQVVDTWQFANPQQQEQALNIASQLRC
                     PQCQNQNLLESNAPVAVSMRHQVYTMVEEGKSEVEIIGWMTERYGDFVRYNPPLTGQT
                     LVLWALPVVLLLLMALILWRVRAKR"
     gene            complement(16289..18007)
                     /locus_tag="J1Y83_000598"
     CDS             complement(16289..18007)
                     /locus_tag="J1Y83_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014640253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase CcmF/NrfE family subunit"
                     /protein_id="HBA7628783.1"
                     /translation="MDVFLPEVGFLALLLSLGVNVLTPLTAFAGVRLRWPAMMRLTCI
                     GILAQFALLLLAFGVLTYCFLISDFSVIYVAQHSYSLLSWELKLAAVWGGHEGSLLLW
                     VLLLSAWSALFAWHYRQQTDPLFPLTLTVLSLILAALLLFVVGWSDPFVRIFPPAIEG
                     RDLNPMLQHPGLIFHPPLLYLGYGGLMVAASVALASLLRGEFDGTCARICWRWALPGW
                     SALTAGIILGSWWAYCELGWGGWWFWDPVENASLLPWLSSTALLHSLSLTRQRGIFRH
                     WSLLLAIVTLMLSLLGTLIVRSGILVSVHAFALDNVRAVPLFSLFALISLASLALYGW
                     RARDGGPAVRFSGLSREMLILATLLLFCAVLLIVLVGTLYPMIYSLLGWGRLSVGAPY
                     FNRATLPFALLMLVVIVLATFVSGKRAQLPALLAHAGVLLFAAGIVVSSVSRQEISLN
                     LQPGQQVTLAGYTFRFERLDLQAKGNYTSEKAIVALFDHQQRIGELTPERRFYEARRQ
                     QMMEPSIRWNGIHDWYAVMGEKTGADRYAFRLYVQSGVRWIWGGGLLMIAGALLSGWR
                     GRKRDE"
     gene            complement(18027..18983)
                     /gene="nrfD"
                     /locus_tag="J1Y83_000599"
     CDS             complement(18027..18983)
                     /gene="nrfD"
                     /locus_tag="J1Y83_000599"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase subunit NrfD"
                     /protein_id="HBA7628784.1"
                     /translation="MTQTSAFHFESLVWDWPIAIYLFLIGISAGLVTLAVLLRRVYPQ
                     AGGADSTLLRTTLIVGPGAVILGLLILVFHLTRPWTFWKLMFHYSFTSVMSMGVMLFQ
                     LYMVVLVLWLAKIFEHDLLALQQRWLPKLGIVQKVLSLLAPVHRGLETLMLVLAVLLG
                     AYTGFLLSALKSYPFLNNPILPVLFLFSGISSGAAVALIAMAIRQRSNPHSTEAHFVH
                     RMEIPVVWGEIFLLVAFFVGLALGDDGKVRALVAALGGGFWTWWFWLGVAGLGLIVPM
                     LLKPWVNRSSGIPAVLAACGASLVGVLMLRFFILYAGQLTVA"
     gene            complement(18980..19651)
                     /gene="nrfC"
                     /locus_tag="J1Y83_000600"
     CDS             complement(18980..19651)
                     /gene="nrfC"
                     /locus_tag="J1Y83_000600"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase Fe-S protein"
                     /protein_id="HBA7628785.1"
                     /translation="MTWSRRQFLTGVGVLAAVSGTAGRVVAKTLNINGVRYGMVHDES
                     LCIGCTACMDACREVNKVPEGVSRLTIIRSEPQGEFPDVKYRFFRKSCQHCDHAPCVD
                     VCPTGASFRDAASGIVDVNPDLCVGCQYCIAACPYRVRFIHPVTKTADKCDFCRKTHL
                     QAGKLPACVEACPTKALTFGNLDDPSSEISQLLRQKPTYRYKLALGTKPKLYRVPFKY
                     GEVSQ"
     gene            complement(19648..20214)
                     /gene="nrfB"
                     /locus_tag="J1Y83_000601"
     CDS             complement(19648..20214)
                     /gene="nrfB"
                     /locus_tag="J1Y83_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418495.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase pentaheme
                     subunit"
                     /protein_id="HBA7628786.1"
                     /translation="MSVLRSLLTAGVLASGLLWSLNGITATPAAQASDDRYEVTQQRN
                     PDAACLDCHKPDTEGMHGKHASVINPNNKLPVTCTNCHGQPSPQHREGVKDVMRFNEP
                     MYKVGEQNSVCMSCHLPEQLQKAFWPHDVHVTKVACASCHSLHPQQDTMQTLSDKGRI
                     KICVDCHSDQRTNPNFNPASVPLLKEQP"
     gene            complement(20259..21695)
                     /gene="nrfA"
                     /locus_tag="J1Y83_000602"
     CDS             complement(20259..21695)
                     /gene="nrfA"
                     /locus_tag="J1Y83_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-forming nitrite reductase cytochrome
                     c552 subunit"
                     /protein_id="HBA7628787.1"
                     /translation="MTRIKINARRIFSLLIPFFFFTSVHAEQTAAPATPVTVEAKNET
                     FAPQHPDQYLSWKATSEQSERVDALAEDPRLVILWAGYPFSRDYNKPRGHAFAVTDVR
                     ETLRTGAPKNAEDGPLPMACWSCKSPDVARLIQKDGEDGYFHGKWARGGPEIVNNLGC
                     ADCHNTASPEFAKGKPELTLSRPYAARAMEAIGKPFEKAGRFDQQSMVCGQCHVEYYF
                     DGKNKAVKFPWDDGMKVENMEQYYDKIAFSDWTNSLSKTPMLKAQHPEYETWTAGIHG
                     KNNVTCIDCHMPKVQNAEGKLYTDHKIGNPFDNFAQTCANCHTQDKAALQKVVAERKQ
                     SINDLKIKVEDQLVHAHFEAKAALDAGATEAEMKPIQDDIRHAQWRWDLAIASHGIHM
                     HAPEEGLRMLGTAMDKAADARTKLARLLATKGITHEIQIPDISTKEKAQQAIGLNMEQ
                     IKAEKQDFIKTVIPQWEEQARKNGLLSQ"
     gene            22087..24045
                     /gene="acs"
                     /locus_tag="J1Y83_000603"
     CDS             22087..24045
                     /gene="acs"
                     /locus_tag="J1Y83_000603"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006816721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HBA7628788.1"
                     /translation="MSQIHKHTIPANIADRCLINPQQYEAMYQQSINAPDTFWGEQGK
                     ILDWITPYQKVKNTSFAPGNVSIKWYEDGTLNLAANCLDRHLQENGDRTAIIWEGDDA
                     SQSKHISYKELHRDVCRFANTLLELGIKKGDVIAIYMPMVPEAAVAMLACARIGAVHS
                     VIFGGFSPEAVAGRIINSSSRLVITSDEGVRAGRSIPLKKNVDDALKNPNVTSVEHVV
                     VLKRTGGKIDWQEGRDLWWHDLVEQASAQHQAEEMNAEDPLFILYTSGSTGKPKGVLH
                     TTGGYLVYAALTFKYVFDYHPGDIYWCTADVGWVTGHSYLLYGPLACGATTLMFEGVP
                     NWPTPARMAQVVDKHQVNILYTAPTAIRALMAEGDKAIEGTDRSSLRILGSVGEPINP
                     EAWEWYWKKIGNEKCPVVDTWWQTETGGFMITPLPGATELKAGSATRPFFGVQPALVD
                     NEGNPLEGVTEGSLVITDSWPGQARTLFGDHERFEQTYFSTFKNMYFSGDGARRDEDG
                     YYWITGRVDDVLNVSGHRLGTAEIESALVAHPKIAEAAVVGIPHNIKGQAIYAYVTLN
                     HGEEPSPELYAEVRNWVRKEIGPLATPDVLHWTDSLPKTRSGKIMRRILRKIAAGDTS
                     NLGDTSTLADPGVVEKLLEEKQAIAMPS"
     gene            24178..24492
                     /locus_tag="J1Y83_000604"
     CDS             24178..24492
                     /locus_tag="J1Y83_000604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF485 domain-containing protein"
                     /protein_id="HBA7628789.1"
                     /translation="MNGTIYQRIEDNAHFRELVEKRQRFATILSIIMLAVYIGFILLI
                     AFAPGWLGTPLNPNTSVTRGIPVGVGVIVISFVLTGIYIWRANGEFDRLNNEVLHEVQ
                     AS"
     gene            24489..26138
                     /gene="actP"
                     /locus_tag="J1Y83_000605"
     CDS             24489..26138
                     /gene="actP"
                     /locus_tag="J1Y83_000605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation/acetate symporter ActP"
                     /protein_id="HBA7628790.1"
                     /translation="MKRVLTALAATLPFAANAADAISGAVERQPTNWQAIIMFLIFVV
                     FTLGITYWASKRVRSRNDYYTAGGNITGFQNGLAIAGDYMSAASFLGISALVFTSGYD
                     GLIYSLGFLVGWPIILFLIAERLRNLGRYTFADVASYRLKQGPIRILSACGSLVVVAL
                     YLIAQMVGAGKLIELLFGLNYHIAVVLVGVLMMMYVLFGGMLATTWVQIIKAVLLLFG
                     ASFMAFMVMKHVGFSFNNLFSEAMAVHPKGVDIMKPGGLVKDPISALSLGLGLMFGTA
                     GLPHILMRFFTVSDAREARKSVFYATGFMGYFYILTFIIGFGAIMLVGANPEYKDAAG
                     HLIGGNNMAAVHLANAVGGNLFLGFISAVAFATILAVVAGLTLAGASAVSHDLYANVF
                     KKGATEREELRVSKITVLILGVIAIILGVLFENQNIAFMVGLAFAIAASCNFPIILLS
                     MYWSKLTTRGAMLGGWLGLITAVVLMILGPTIWVQILGHEKAIFPYEYPALFSISVAF
                     LGIWLFSATDNSAEGARERELFRAQFIRSQTGFGVEQGRAH"
     gene            complement(26177..26866)
                     /locus_tag="J1Y83_000606"
     CDS             complement(26177..26866)
                     /locus_tag="J1Y83_000606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000181101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LrgB family protein"
                     /protein_id="HBA7628791.1"
                     /translation="MANFQLSMLCLAITLVIYFLNKRLYRRFRKLPLMPLVLTPTLLV
                     MLLVFGNISWQNYIGEAHWLLWLLGPATIAFAVPVYDNRAIIKRHWMSLTAGVLTATV
                     VAVTSSVWLARLFTLPDEIQRSLAVRSVTTPFALAASQSLGGQPDLVALFVVVTGVFG
                     MAIGDVLFLRLSIREGMAKGAGFGAASHGAGTARSYELGQQEGVVASLVMMLSGVLMV
                     LVSPLVARMMF"
     gene            complement(26859..27269)
                     /locus_tag="J1Y83_000607"
     CDS             complement(26859..27269)
                     /locus_tag="J1Y83_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001143718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HBA7628792.1"
                     /translation="MPVALRRITPAVVQSIQVFFQVVLYAGLFIFAQYLVSWLHLPLP
                     ANLVGMILMLALIVCRILPLQWVRAGARWLLAEMLLFFVPAVVAVVNYAQLLLVDGWR
                     IFAVIALSTVMVLGTTAWVVEKVYRYEMRRLNRG"
     gene            27373..28257
                     /locus_tag="J1Y83_000608"
     CDS             27373..28257
                     /locus_tag="J1Y83_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016159883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBA7628793.1"
                     /translation="MDIRTLRYFVEVVRQQSFTRAAEKLFVTQPTISKMLKNLEDELN
                     CILLIRDGRRLLLTDTGRVVFERGLALLAEFRQLEAELSDINQLKTGVLRLGIPPMVG
                     MLMAGPIGLFRQRYPGVELKISEFGGLTVQQAVMNGELDMALTALPVEESGLTTLSLF
                     SHPLCVLTPHSGKWLECDAVSPETLAEHPLLIYNEDFALSKQLMKLFDEHGVKPRIAV
                     RSGQWDFLAAMVQAGVGIAILPEPICQRLDKNTLKWLPLESELSWKLGMIWREGVYLS
                     HSAHAWLSCCEGYWLTPE"
     gene            complement(28293..29942)
                     /locus_tag="J1Y83_000609"
     CDS             complement(28293..29942)
                     /locus_tag="J1Y83_000609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter"
                     /protein_id="HBA7628794.1"
                     /translation="MEIFFTILIMTLVVSLSGVVTRVMPFQIPLPLMQIAIGALLAWP
                     TFGLHVEFDPELFLVLFIPPLLFADGWKTPTREFLEHGREIFGLALALVVVTVVGIGF
                     LIYWVVPGIPLIPAFALAAVLSPTDAVALSGIVGEGRIPKKIMGILQGEALMNDASGL
                     VSLKFAVAVAMGTMIFTVGGATVEFMKVAIGGILAGFVVSWLYGRSLRFLSRWGGDEP
                     ATQIVLLFLLPFASYLIAEHIGVSGILAAVAAGMTITRSGVMRRAPLAMRLRANSTWA
                     MLEFVFNGMVFLLLGLQLPGILETSLMAAEIDPNVEIWMLFTDIILIYAALMLVRFGW
                     LWTMKKFSNRFLKKKPMEFGSWTTREILIASFAGVRGAITLAGVLSIPLLLPDGNVFP
                     ARYELVFLAAGVILFSLFVGVVMLPILLQHIEVADHSQQLKEERIARAATAEVAIVAI
                     QKMEERLAADTEENIDNQLLTEVSSRVIGNLRRRADGRNDVESSILEENLERRFRLAA
                     LRSERAELYHLRATREISNETLQKLLHDLDLLEALLIEENQ"
     gene            complement(30093..31442)
                     /locus_tag="J1Y83_000610"
     CDS             complement(30093..31442)
                     /locus_tag="J1Y83_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HBA7628795.1"
                     /translation="MSTPSARTGGSLDAWFKISQRGSTVRQEVVAGLTTFLAMVYSVI
                     VVPGMLGKAGFPPAAVFVATCLVAGLGSIVMGLWANLPLAIGCAISLTAFTAFSLVLG
                     QHISVPVALGAVFLMGVLFTVISATGIRSWILRNLPHGVAHGTGIGIGLFLLLIAANG
                     VGLVIKNPLDGLPVALGDFTTFPVMMSLVGLAVIIGLEKLKVPGGILLTIIGISIVGL
                     IFDPNVHFSGVFAMPSLSDENGNSLIGSLDIMGALNPVVLPSVLALVMTAVFDATGTI
                     RAVAGQANLLDKDGQIIDGGKALTTDSMSSVFSGLVGAAPAAVYIESAAGTAAGGKTG
                     LTAITVGVLFLLILFLSPLSYLVPGYATAPALMYVGLLMLSNVAKIDFADFVDAMAGL
                     VTAVFIVLTCNIVTGIMIGFATLVIGRLVSGEWRKLNIGTVVIAVALVTFYAGGWAI"
     gene            complement(31990..32454)
                     /gene="soxR"
                     /locus_tag="J1Y83_000611"
     CDS             complement(31990..32454)
                     /gene="soxR"
                     /locus_tag="J1Y83_000611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensitive transcriptional activator SoxR"
                     /protein_id="HBA7628796.1"
                     /translation="MEKKLPRIKALLTPGEVAKRSGVAVSALHFYESKGLITSIRNSG
                     NQRRYKRDVLRYVAIIKIAQRIGIPLATIREAFGVLPEGHTLSAKEWKQLSSQWREEL
                     DRRIHTLVALRDELDGCIGCGCLSRSDCPLRNPGDRLGEEGTGARLLEDEQN"
     gene            32540..32863
                     /gene="soxS"
                     /locus_tag="J1Y83_000612"
     CDS             32540..32863
                     /gene="soxS"
                     /locus_tag="J1Y83_000612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide response transcriptional regulator
                     SoxS"
                     /protein_id="HBA7628797.1"
                     /translation="MSHQKIIQDLIAWIDEHIDQPLNIDVVAKKSGYSKWYLQRMFRT
                     VTHQTLGDYIRQRRLLLAAVELRTTERPIFDIAMDLGYVSQQTFSRVFRRQFDRTPSD
                     YRHRL"
     gene            complement(32899..34452)
                     /gene="pdeC"
                     /locus_tag="J1Y83_000613"
     CDS             complement(32899..34452)
                     /gene="pdeC"
                     /locus_tag="J1Y83_000613"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="c-di-GMP phosphodiesterase PdeC"
                     /protein_id="HBA7628798.1"
                     /translation="MSHRARHQLLAFPGIIFLVLFPIIMSLWIAFFWAKSEVNNQLRT
                     FAQLALDKSELVIRQADLVSDAAERYQGQVCTPAHQKRMLNIIRGYLYINELIYARDN
                     HFLCSSLIASVNGYTIAPADYKREPNVSIYYYRDTPFFSGYKMTYMQRGNYVAVINPL
                     FWSEVMSDDPTLQWGVYDTVTKTFFSLSNEASAATFSPLIHLNDLTVQRNGYLYATVY
                     STKRPIAAIVATSYQRLITHFYNHLIFALPAGILGSLVLLLLWLRIRQNYLSPKRKLQ
                     RALEKHQLCLYYQPIIDIKTEKCIGAEALLRWPGEQGQVMNPAEFIPLAEKEGMIEQV
                     TDYVIDNVFRDLGAYLATHTDRYVSINLSASDFHTSRLIARTNQKTEQHAVRPQQIKF
                     EVTEHAFLDVDKMTPIILAFRKAGYEVAIDDFGIGYSNLHNLKSLNVDILKIDKSFVE
                     TLTTHKTSHLIAEHIIELAHSLGLKTIAEGVETEAQVNWLRKRGVRYCQGWFFAKAMP
                     PQVFMQWMG"
     gene            34881..35162
                     /locus_tag="J1Y83_000614"
     CDS             34881..35162
                     /locus_tag="J1Y83_000614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA7628799.1"
                     /translation="MATLTTGVVLLRWQLLSAVMMFLASTLNIRFRRADYVGLAVISS
                     GLGVVSACWFAMGLLGITMADITAIWHNIESVMVEEMNQTPPQWPMILT"
     gene            complement(35261..35797)
                     /gene="ssb1"
                     /locus_tag="J1Y83_000615"
     CDS             complement(35261..35797)
                     /gene="ssb1"
                     /locus_tag="J1Y83_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein SSB1"
                     /protein_id="HBA7628800.1"
                     /translation="MASRGVNKVILVGNLGQDPEVRYMPNGGAVANITLATSESWRDK
                     ATGEMKEQTEWHRVVLFGKLAEVASEYLRKGSQVYIEGQLRTRKWTDQSGQDRYTTEV
                     VVNVGGTMQMLGGRQGGGAPAGGNIGGGQPQGGWGQPQQPQGGNQFSGGAQSRPQQST
                     PAAPSNEPPMDFDDDIPF"
     gene            36052..38874
                     /gene="uvrA"
                     /locus_tag="J1Y83_000616"
     CDS             36052..38874
                     /gene="uvrA"
                     /locus_tag="J1Y83_000616"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HBA7628801.1"
                     /translation="MDKIEVRGARTHNLKNINLVIPRDKLIVVTGLSGSGKSSLAFDT
                     LYAEGQRRYVESLSAYARQFLSLMEKPDVDHIEGLSPAISIEQKSTSHNPRSTVGTIT
                     EIHDYLRLLYARVGEPRCPDHDVPLAAQTVSQMVDNVLSQPEGKRLMLLAPIIKERKG
                     EHTKTLENLASQGYIRARIDGEVCDLSDPPKLELQKKHTIEVVVDRFKVRDDLTQRLA
                     ESFETALELSGGTAVVADMDDPKAEELLFSANFACPICGYSMRELEPRLFSFNNPAGA
                     CPTCDGLGVQQYFDPDRVIQNPELSLAGGAIRGWDRRNFYYFQMLKSLADHYKFDVEA
                     PWGSLSANVHKVVLYGSGKENIEFKYMNDRGDTSIRRHPFEGVLHNMERRYKETESSA
                     VREELAKFISNRPCASCEGTRLRREARHVYVENTPLPTISDMSIGHAMEFFNNLKLAG
                     QRAKIAEKILKEIGDRLKFLVNVGLNYLTLSRSAETLSGGEAQRIRLASQIGAGLVGV
                     MYVLDEPSIGLHQRDNERLLGTLIHLRDLGNTVIVVEHDEDAIRAADHVIDIGPGAGV
                     HGGEVVAEGPLEAIMAVPESLTGQYMSGKRKIEVPKKRVPANPEKVLKLTGARGNNLK
                     DVTLTLPVGLFTCITGVSGSGKSTLINDTLFPIAQRQLNGATIAEPAPYRDIQGLEHF
                     DKVIDIDQSPIGRTPRSNPATYTGVFTPVRELFAGVPESRARGYTPGRFSFNVRGGRC
                     EACQGDGVIKVEMHFLPDIYVPCDQCKGKRYNRETLEIKYKGKTIHEVLDMTIEEARE
                     FFDAVPALARKLQTLMDVGLTYIRLGQSATTLSGGEAQRVKLARELSKRGTGQTLYIL
                     DEPTTGLHFADIQQLLDVLHKLRDQGNTIVVIEHNLDVIKTADWIVDLGPEGGSGGGE
                     ILVSGTPETVAECEASHTARFLKPML"
     gene            complement(38909..39265)
                     /locus_tag="J1Y83_000617"
     CDS             complement(38909..39265)
                     /locus_tag="J1Y83_000617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HBA7628802.1"
                     /translation="MTISELLQYCMAKPGAEQSVHNDWKATQIKVEDVLFAMVKEVEN
                     RPAVSLKTSPELAELLRQQHSDVRPSRHLNKAHWSTVYLDGSLPDSQIYYLVDASYQQ
                     AVNLLPEEKRKLLVQL"
     gene            complement(39269..39685)
                     /locus_tag="J1Y83_000618"
     CDS             complement(39269..39685)
                     /locus_tag="J1Y83_000618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbQ family protein"
                     /protein_id="HBA7628803.1"
                     /translation="MWYQKTLTLSAKSRGFHLVTDEILNQLADMPRVNIGLLHLLLQH
                     TSASLTLNENCDPTVRHDMERFFLRAVPDNGNYEHDYEGADDMPSHIKSSMLGTSLVL
                     PVHKGRIQTGTWQGIWLGEHRIHGGSRRIIATLQGE"
     gene            complement(39796..40509)
                     /gene="aphA"
                     /locus_tag="J1Y83_000619"
     CDS             complement(39796..40509)
                     /gene="aphA"
                     /locus_tag="J1Y83_000619"
                     /EC_number="3.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid phosphatase AphA"
                     /protein_id="HBA7628804.1"
                     /translation="MRKITQALSAVCLLFALNSSAVALASSPSPLNPGTNVAKLAEQA
                     PIHWVSVAQIENSLAGRPPMAVGFDIDDTVLFSSPGFWRGKKTFSPESEDYLKNPVFW
                     EKMNNGWDEFSIPKEVARQLIDMHVRRGDAIFFVTGRSPTKTETVSKTLADNFHIPAT
                     NMNPVIFAGDKPGQNTKSQWLQDKNIRIFYGDSDNDITAARDVGARGIRILRASNSTY
                     KPLPQAGAFGEEVIVNSEY"
     gene            40786..42606
                     /locus_tag="J1Y83_000620"
     CDS             40786..42606
                     /locus_tag="J1Y83_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000704417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HBA7628805.1"
                     /translation="MKISWNYIFKNKWRFHITSISLFLIMLAVSIAFLHLRFNTLSGT
                     DKMRLEMYKSTLYSTIEQFYVLPYMLSTDHIIRQAVITPDDMTSSELNQRIAHFNTQL
                     KTAAIFILDTQGKAIASSNWQDPGSYVGQNYSYRPYYKHAMSGLNGRFYGIGSTTNTP
                     GFFLSTSIKDKGKIVGVVVVKISLNEIEKAWAEGPENIIVNDEHGIIFLSSKSPWRMR
                     TLQPLPVQAKQKLQSTRQYSLDNLLPADYYPCYTVSNFTFLKDKKEQLCLFPQYYTQQ
                     IAIPEFNWKMTIMVPLDNLYWSWAISLVITLIIYLLFLLFIKYWRMRSHAQQLLTLAN
                     ETLEKQVKERTSALELINQKLIQEIKERSQAEQVLQITRSELAESSKLAALGQMATEI
                     AHEQNQPLAAIHALTDNARTMLKKEMYPQVEQNLKHIISVIERMTQLISELKAFASRH
                     RVPKGSADVIKVMYSAVALLNHSMEKNNIERRIKAPSMPLFVNCDELGLEQIFSNLIS
                     NALDSMEGSSYKRLDIAIRQANNKVIITIKDSGGGFAPEVVDRIFEPFFTTKRRGMGL
                     GLAIVSEIVRNSNGALHASNHPEGGAVMTLTWPEWGEEHE"
     gene            42599..43957
                     /locus_tag="J1Y83_000621"
     CDS             42599..43957
                     /locus_tag="J1Y83_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019841943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent Fis family transcriptional
                     regulator"
                     /protein_id="HBA7628806.1"
                     /translation="MNNKSSEITIVYIEDSDDVRFACEQTLTLAGYRVISCCDAEHSI
                     PLIQSQANIIILTDVRLPGISGLELLSYINEMDSKIPVILITGHGDVEMAVDAMRNGA
                     FDFIEKPSSSDKLLSIIARAVEKRRLVLENQQLLANLQQENGPVLIGRSPQMQQLRKM
                     ILNVADTGADVLIYGETGCGKEVVARMLHHWSTRRQGQFVALNCAGLPETLFESEIFG
                     HEAGAFTGAVKKRIGKIEHANGGTLFLDEIEGMPSGMQVKLLRVLQERTIERLGANQL
                     IPVNCRVIAATKEDLLRRSEEHLFRLDLYYRLNVVSLNIPPLRQRREDIPELFYWFAS
                     QAAQKYNRPLPDISPMLLAWLQSQSWPGNVRELKHNAERFVLGLLTHHQPVPMTQQEE
                     SGLTACIDAFEKKLIEDMLRQTEGQVSLTARLLQLPRKTLYDKLNKHQIQPQVYRPES
                     SS"
     gene            complement(43946..45019)
                     /locus_tag="J1Y83_000622"
     CDS             complement(43946..45019)
                     /locus_tag="J1Y83_000622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001304711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ldh family oxidoreductase"
                     /protein_id="HBA7628807.1"
                     /translation="MSLQIAVEKVRWLAAGLLELNGCDADIAQDVAVHMIEAERYGFA
                     SHGVTLLPKYLENIARGDVTANARPECLTSEGNLQRFHAHNGFGQHAGKVAVNAAIEQ
                     AKHRGFCLLTLCHAHHLGRMGHYGQMVADQGLAMLAMSNVTGRPALVAPWGGAEPRMT
                     TNPFCFAWPFSDGRPPILVDFATSSMALNKARVMSSTGKQAAPGQLIDAQGNPSNDPG
                     VLFSNPPGALLPFGEHKGFGLALMIEMMAGILSGGDTIAEDHQTDGSAHNHLFALIID
                     PDQFTDLAGEKGQFFADYLLATQPQPGGNPVIYPGMPEAANRERNAKMITIPVSFWSW
                     VVEHYARKGIDLGLHPQESALAG"
     gene            complement(45031..46530)
                     /locus_tag="J1Y83_000623"
     CDS             complement(45031..46530)
                     /locus_tag="J1Y83_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001296642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DASS family sodium-coupled anion symporter"
                     /protein_id="HBA7628808.1"
                     /translation="MSSISHGAPQKRRIIPNPGLWLAIIAGIIITLLPLGDTLPVAGQ
                     NMIAILVFAIIVWISEAMDYTASAIVISALIIFMVGFAPDMNHPDTILGTAKALKMTL
                     SGFSNSALALVAAAMFIAAAMTITGLDKRIALFTMSKIGASSRSIIIGAIVVTIVLSL
                     VVPSATARTACVVPIMMGVIAAFKVDKHSRLAASMMIVIAQATSIWNVGIQTSAAQNL
                     LSIGFINKTFGAGHSVSWLDWLLAGAPWSLTMSVILYFLARKLLPPETEAVEGGSEAI
                     KKALAELGPTTGKEKRLIGISLLLLLFWSTGGKLHSIDTTSVTLAGLAIMLLPGIGVM
                     NWKEVEKRVQWGTLLMFGIGISLGSTLLDTQAASWMANYVVKGFGLDGLPSLAIFAIL
                     AAFLIIIHLGFASATALTAALLPILISLLSSLPPELGVNPVGMTILLAFSVSFGFILP
                     INAPQNMVCMGTDTFTPRQFTRVGLYLTVIGYLLLLLFAATWWKILGLM"
BASE COUNT        11673 a        12351 c        11783 g        10772 t
ORIGIN      
        1 catcgagaaa atagccatac tgacgccttc aatggatatt gatattcact acagatattg
       61 ttcgcagcag acaggatgta tttaatatgc caacagttct ctctcgcatg gcgatgcaac
      121 tcaaaaaaac agcctggata attcccgtct tcatggtttc gggatgctca ttatctccgg
      181 caatcccggt gatcggcgct tattatcccg gctggttttt ctgcgccatt gccagcctta
      241 ttttgacgct catcacgagg cgaattattc agcggacaaa tatcaatctg gcatttgtcg
      301 gaattattta taccgccctt tttgctctct acgccatgct gttctggctg gcatttttct
      361 aatctatttg agacgcgctc atggaaagta cgccgaaaaa agctcctcgc agtaaattcc
      421 ctgctctgtt agtggttgcg ttggcgctgg ttgcccttgt tttcgttatc tggcgcgtag
      481 acagtgcgcc atcaactaat gacgcttacg cgtcagcaga taccattgat gtggtgccgg
      541 aagtcagcgg tcgcattgta gaactggcgg tcaccgacaa ccaggcggtg aagcagggcg
      601 atttgctgtt ccgcattgac ccgcgcccgt acgaagccaa tctggcgaaa gctgaagcct
      661 ccctcgcggc gctggataag caaattatgc tcacccagcg tagcgttgac gcacaacagt
      721 ttggtgccga ctcggttaat gccacggtag aaaaagcccg tgccgccgcg aaacaagcca
      781 gcgatacgtt acgccgtacc gaaccgctac tgcgagaagg ttttgtttct gcggaagagg
      841 ttgaccgtgc aagaaccgca cagcgtgcgg cagaagcgga ccttaatgcc gtattgttgc
      901 aggcgcagtc agccgccagc tccgtcagcg gcgtggatgc gctggtggcg caacgtgcgg
      961 cagtcgaagc ggatattgcg ttgacgaaac tgcatctgga aatggcgacc gttcgcgcgc
     1021 cgtttgatgg ccgggtcatt tccctcaaaa cctccgtcgg gcagtttgca tctgcgatgc
     1081 gccctatttt cactctaatc gacactcgtc actggtacgt gattgccaac ttccgcgaaa
     1141 ccgatctgaa aaatattcgc tcgggtacac tcgcaaccat tcgcctgatg agcgacagcg
     1201 gcaaaacctt cgagggtaaa gtggattcga ttggctacgg cgtgctaccg gatgacggcg
     1261 gcctggtgct gggcggcctg ccgaaagtgt cacggtctat taactgggtc cgcgttgccc
     1321 agcgttttcc ggtcaaaatc atggttgata aacctgaccc ggaaatgttc cgcatcggcg
     1381 cttcggcagt cgctaatctt gagccacaat aatgagcgcg ctcaactccc tgccattacc
     1441 ggtggtcagg ctgctggcgt tctttcatga agagttaagc gagcggcgac cagggcgcgt
     1501 gccacagact atgcaactct gggtaggctg cctgctggtg attctgatct cgatgacttt
     1561 tgagatccct tttgtggcgt tatcactggc agtgctgttt tacggtattc agtcgaacgc
     1621 gttttacacc aaatttgtcg cgatcttgtt tgtggttgcc acggtgctgg agatcggcag
     1681 cctgtttttg atctacaaat ggtcatatgg cgaaccgttg atccgattga tcatctccgg
     1741 gccgatcctg atgggctgca tgtttttgat gcgcacccat cggctgggac tggtcttttt
     1801 cgccgtcgcc attgtcgcta tttacgggca aaccttcccc gccatgctcg actatccgga
     1861 agtggtcgtg cgcttaacgc tgtggtgtat cgttgttggc ctctatccaa ccttgctgat
     1921 gacgttaatc ggcgtgctgt ggtttcccag tcgtgccatt acgcagatgc atcaggcgct
     1981 taatgatcgg cttgatgatg ccattagcca cctgacggac agcctcgcac cgctacccga
     2041 aacgcggatt gaaagagagg cgctggcgct gcaaaaactc aatgtctttt gcctcgcgga
     2101 tgatgccaac tggcgaaccc agagcgcatg gtggcaaagc tgcgtggcaa cggtaaccta
     2161 catttactcg acgctgaatc gctacgatcc cacctctttt gctgattctc aggcaattat
     2221 tgaatttcgg caaaaattag tctcagaaat caacaagctg caacatacca ttgccgaagg
     2281 tcagtgctgg caaagcgact ggcggcttag tgaagatgaa gcggtggcgg cacgagaatg
     2341 taacctggag aatatctgcc agacgttatt gcaactgggt cagatggacc cgaatacgcc
     2401 gcctacgcct gcagccaagc cgccatcaat ggtcgccgat gcctttacca atccagacta
     2461 tatgcgctac gcggtaaaaa cgctgctcgc ctgtttgatc tgttacacct tctacagcgg
     2521 cgtggactgg gaaggcattc acacctgtat gctgacctgc gtgatcgtcg ctaatcccaa
     2581 tgtcggttcg tcgtaccaga agatggtgct gcgttttggc ggggcctttt gcggcgcgat
     2641 tctggcgctg ttattcacgt tattggtcat gccctggctg gacaatattg tcgaattgct
     2701 gtttgtgctg gcaccgattt tcctgttggg cgcatggatt gccaccagct ctgaacgctc
     2761 ttcttatatc ggcacacaga tggtggtcac cttcgcgctc gccacgctcg aaaacgtttt
     2821 tggcccggtg tacgacctgg tggaaattcg cgatcgcgcc ctgggtatca tcattggtac
     2881 cgtggtgtcc gcggtgattt acacctttgt ctggcctgaa agcgaagcgc gcacgctgcc
     2941 gcaaaaactg gctggcgcgc tgggtatgtt gagtaaagta atgcggatcc cacgccagca
     3001 ggaagtcacg gctctgcgca cttatctgca aattcgcata ggtctgcatg cggcgtttaa
     3061 tgcctgtgaa gagatgtgcc aacgcgtggt gctggagcgt caactggaca gcgaagaacg
     3121 cgcattactg attgaacgtt cgcaaacggt tattcgtcag ggccgcgata ttcttcacgc
     3181 ctgggatgcc acctggaact cggcgcaggc gctggataac gcactacagc cggacagagc
     3241 aggtcagttt gccgacgccc tggagaaata cgccgccggt ctggcaaccg cactcagccg
     3301 ttctcctcaa ataacgcttg aagaaacacc cgcctcgcag gccatcctgc cgaccttatt
     3361 aaaacaggag caacacgtct gccagctttt cgcccgcttg ccagactgga cagccccggc
     3421 attaacgccc gccacggaac aggcacaagg agccacgcaa tgatcaatcg tcaactttca
     3481 cgcctgctgt tgtgcagcat tctcggcagc acgacgctga tttccggctg tgccctggta
     3541 cgtaaggatt ctgcgcctca tcaacagctc aaaccggaac aaatcaaact ggccgatgat
     3601 attcatcttg ccagctccgg ctggccgcag gcgcagtggt ggaaacaatt taacgacccg
     3661 cagctggatg cgctgatcca acggacgcta agtggttcac accccctcgc cgaagcgaaa
     3721 ctgcgggaag aaaaagcgca gtcccaggcc gatttgttag atgccggttc acaattacag
     3781 gtcgcagcgt tagggatgct caaccgccaa cgcgtctcgg cgaacggctt tttaagccct
     3841 tatgcgatgg atgcgccagc actgggtatg gacgggccgt actatacgga agccacagtc
     3901 ggtttgtttg ccggactgga tctcgatttg tggggtgtgc atcgctcagc ggttgctgcc
     3961 gccattggcg cgcataatgc cgcgctggca gaaaccgcag cagtagagct atcgctgacc
     4021 acgggcgtag cgcagcttta ttacagtatg caggccagct atcagatgct cgatctgtta
     4081 gaacaaactc gcgatgtgat tgattacgcg gtgaaagcgc accagagtaa agtggcgcac
     4141 ggtctggaag cgcaagtgcc tttccacggc gcgcgggcac agattctggc ggtcgataaa
     4201 caaattgctg ccgtcaaagg gcaaatcact gaaacgcgag aatctctgcg tgcattgatt
     4261 ggcgcgggag ccagcgatat gccggagatc aaaccggtgg cattaccacg agtacagacc
     4321 ggcattccgg caacgctctc ttatgagttg ctcgccagac gcccggatct gcaagccatg
     4381 cgctggtatg ttcaggcgtc attagatcag gtggattccg cgcgggcgct gttctacccg
     4441 agctttgata tcaaagcgtt tttcggtctg gactccatcc acctggatac cttattcaaa
     4501 aaaaccagtc gccagttcaa cttcatcccg ggtctgaaat tgccgctgtt tgacggtgga
     4561 cggttgaatg ccaatctcga aggcactcgc gccgccagca acatgatgat tgaacgttac
     4621 aaccagtcag tactgaacgc ggtgcgtgac gttgccgtca acggcacacg tctgcaaacg
     4681 ctcaacgacg agcgagagat gcaggctgaa cgcgtagaag caacgtgctt cacccagcgc
     4741 gcagccgagg ccgcctatca gcgcggctta accagccgct tacaggccac cgaagcccgg
     4801 ttgccagtgc ttgccgagga gatgtcatta ctgatgctgg acagccgccg ggtgatccaa
     4861 agcattcagt tgatgaaatc gctgggcggc gggtatcaga cggctcccgt cgtcgagaaa
     4921 aaataaaatg tctaccgcgt gatggctgtc acgcggtatt tcgtttcgtc acgtcaaaac
     4981 tgacgacagc ctgtttttcg tcagagtttt gaataaatag tgcccgtaat atcagggaat
     5041 gaccccaaat aaaatgtggc ataaaagatg catactgtag tcgagagcgc gtatgcgtga
     5101 tttgattaac tggagcgaga ccgatgaaaa aagtcgtcac ggtttgcccc tattgcgcat
     5161 caggttgcaa aatcaacctg gtcgtcgata acggcaaaat cgtccgggcg gaggcagcgc
     5221 aggggaaaac caaccagggt accctgtgtc tgaagggtta ttatggctgg gacttcatta
     5281 acgataccca gatcctgacc ccgcgcctga aaacccccat gatccgtcgc cagcgtggcg
     5341 gcaaactcga acctgtttcc tgggatgagg cactgaatta cgttgccgag cgcctgagcg
     5401 ccatcaaaga gaagtacggt ccggatgcca tccagacgac cggctcctcg cgtggtacgg
     5461 gtaacgaaac caactatgta atgcaaaaat ttgcgcgcgc cgttattggt accaataacg
     5521 ttgactgctg cgctcgtgtc tgacacggcc catcggttgc aggtctgcac caatcggtcg
     5581 gtaatggcgc aatgagcaat gctattaacg aaattgataa taccgattta gtgttcgttt
     5641 tcgggtataa cccggcggat tcccacccaa tcgtggcgaa tcacgtaatt aacgctaaac
     5701 gtaacggggc gaaaattatc gtctgcgatc cgcgcaaaat tgaaactgcg cgcattgctg
     5761 acatgcacat tgcattgaaa aacggctcga acatcgcgct gttgaatgcg atgggccatg
     5821 tcattattga agaaaatctg tacgacaaag cgttcgtcgc ttcccgtaca gaaggctttg
     5881 aagagtatcg taaaatcgtt gaaggctaca cgccggagtc ggttgaagat atcaccggcg
     5941 tcagcgccag tgagattcgt caggcggcgc ggatgtatgc ccaggcggaa aacgccgcca
     6001 tcctgtgggg catgggggta acccagttct atcagggcgt ggaaaccgtg cgttctctga
     6061 ccagcctcgc gatgctgacc ggtaacctcg gtaagccaca tgcgggtgta aacccggttc
     6121 gtggtcagaa caacgtgcag ggtgcctgcg atatgggcgc gctgccggat acgtatccgg
     6181 gctatcagta cgtgaaagat ccggctaacc gcgagaaatt cgccaaagcc tggggcgtgg
     6241 aaagcctgcc tgctcatacc ggctatcgca tcagcgagct gccgcaccgc gtggcgcatg
     6301 gtgaagtgag agcggcgtac attatgggcg aagatccgct gcaaactgac gcggaactgt
     6361 cggcagtacg taaagccttt gaagatctgg aactggttat cgttcaggac atctttatga
     6421 ccaaaaccgc gtcggcggcg gatgtcattt taccgtcaac gtcgtggggc gagcatgaag
     6481 gcgtgtttac tgcggctgac cgtggcttcc agcgtttctt caaggcggtt gagccgaagt
     6541 gggatctgaa aacggactgg caaatcatca gtgaaattgc cactcgtatg ggttatccga
     6601 tgcactacaa caacacccag gagatctggg acgagttgcg tcatctgtgc ccggatttct
     6661 acggtgcgac ttacgagaaa atgggcgaac tgggcttcat tcagtggcct tgccgcgata
     6721 cttcagatgc cgatcagggg acgtcttatc tgtttaaaga gaagtttgat accccgaacg
     6781 gtctggcgca gttcttcacc tgcgactggg tagcgccaat cgacaaactc accgacgagt
     6841 acccgatggt actgtcaacg gtgcgtgaag ttggtcacta ctcttgccgt tcgatgaccg
     6901 gtaactgtgc ggcactggcg gcgctggctg atgaacctgg ctacgcacaa atcaataccg
     6961 aagacgccaa acgtctgggt attgaagatg aggcattggt ttgggtgcac tcgcgtaaag
     7021 gcaaaattat cacccgtgcg caggtcagcg atcgtccgaa caaaggggcg atttacatga
     7081 cctaccagtg gtggattggt gcctgtaacg agctggttac cgaaaactta agcccgatta
     7141 cgaaaacgcc ggaatacaaa tactgcgccg tgcgcgtcga gccgatcgcc gatcagcgcg
     7201 ccgccgagca gtacgtgatt gacgagtaca acaagttgaa aactcgcctg cgcgaagcgg
     7261 cactggcgta atatcgcgta aactcacgtt aaaagcggcg atattaccca acgcctttgt
     7321 atcaatccgg aaacgactct ctgtttccgg atttttttat acatacctgc gcagtcaaac
     7381 cagcccatcc tggttattag cgacgtcttc tcggttgttc ttctattcca gtcacgattg
     7441 caaatgagaa ttatctttac tcaattctca tcgcggccta acgtaataat actttacatt
     7501 tgataaacaa aatttcggta aggtgagaaa tcattgaccg tttaactcgt tctattaacg
     7561 ggatattctg gcgcgattaa tgcagcgaaa ccccgtctga tagcatatta ataacctcaa
     7621 agtaatttca gggattggga agcatatgat taaaaaacta ttacctttac tggttttaag
     7681 cacaatttcc gccacaaccg ttgccgctac accgcctaat acgttggttg tagcccaggg
     7741 gctggatgat attgtgagtc tggacccggc agaagctaat gaactctcaa gtattcagac
     7801 tgtaccaagt ctgtatcaac gcgtagtaca accggaccgt aatgatccga cgaaaatcac
     7861 tccgattctg gcggaaagtt ggcaggctga ccccgcagcc aaaacgctaa ccataaaact
     7921 gaaaagcgat gcgaaatttg cctctggtaa tccggtacgt ccggaagatg tgatttactc
     7981 ttatgtccgg gctgtcacac tgaataaatc cggtgcgttt attctcaatg tgctgggatg
     8041 gcagccagaa aatatcgcca gccagttgaa gaaaatcgat gaccataccg tccaggtgca
     8101 atggagtgcc gacgtcagct cggcgctggc actgaatatt ctctccacac caatcgcctc
     8161 aattgttgac gagaagctgg ttgcgccaaa cgccaaaaat aacgactttg gtaatgaatg
     8221 gctgaagatg cattccgctg gcagcggcgc gtttaaaatg cgtacttacc aaccgcatca
     8281 ggccatcgtg atggaagcca acgccagctc accaaccggc gccccgaaac tgaaaaacgt
     8341 catcatcaaa aatgttcccg atccggcaac gcgtcgcctg ctgatccaac aaggtgatgc
     8401 tgatatggct cgtgatctgg gagccgatca aatcgatgca ttacagggta aacctggcgt
     8461 aaaagtgatg agcattgcct ccgccgaaca gaactacctg gccttcaata ccggtaacaa
     8521 ggataacccg ctgatgagta atcctgcgtt ctgggaggcc gctcgctggt tggtcgatta
     8581 cgacggcatc accaaaaatc tcttaaaagg acaatatttt acccatcaaa gtttcctgcc
     8641 cgtgggcttt cctggagcac tggaagaaac accgtttacc tttaatccag ccaaagcaaa
     8701 agagatcctc gctaaagccg gaatcaaaga cccgcacttc acgcttgatg tcgaaaataa
     8761 accgccattt attaccatcg cgcagtcaat tcaggccagc tttgctcagg gcggtgtaaa
     8821 agttgatttg ctgccagccg cagggagtca ggtctactct cgcgttcgcg cccaccagca
     8881 tcagggggcc attcgtatgt ggttgcctga ttattttgac gcacattcca acgccagctc
     8941 tttcgcctat aacgatggca aatccagcac cgttgccggg ttaaacggct ggaaaatccc
     9001 ggaactgaac aaagaaacgc tggcggccat cgctgaacct gatagcacta aacgtcttga
     9061 tttgtataag aaaatgcaac aagaactaca acgtagttcc ccgtatgtct ttattgatca
     9121 aggcaaaacg caaattgtca tgcgtgataa tgtgcagggg tatcaacaag ggttgaatgc
     9181 cgatatggtc tggtttgatc aggtaaccaa ataaccctgt atgcaactcg cgtccagcca
     9241 tgttaccggg cgcggtacca gggctggcct gtattgcagg ccagccgctg atttatcact
     9301 gtatggaaag atcatgtctg tcgttttttc cactcgcgcg agatggcgag gcaagcgccc
     9361 tctgctcgcg ctgttgcaag ggctgtttac cgttgctctg acgctactcg gcctgctgct
     9421 gattaccttt gcgctttcgg ctctctcacc ggtcgatcgc gtactacaaa tcgttggcga
     9481 tcacgccagc cagtccacct atgatcaggt acgccaccag ttaggattgg atcagccgtt
     9541 agccgtccag ttctggcatt acctgatgaa tctggcacac ggcgatctgg gcatcgccag
     9601 ctccaccggg caaccagtac tccaggactt gctatcagca ttcccggcga ccattgaact
     9661 ggcaacgttg gcgcttattg tcggtgctgt tgtcggcgtc attgcgggtg tactttgcgc
     9721 ccgttatgtc ggttctccgc tggatttcac cgtgcgaaca ttaactctac tgggaaattc
     9781 cgtccctgtt ttttggcttg gtctgctgat gctggcgctg ttctacgcca gactgcaatg
     9841 gagtgccggg ccgggcaggc tggatgatat ttatcaattc accatagaac cccgcagcgg
     9901 atttgcgctg attgatacgt ggctttctgg cgatcgcgag gcatttacca atgcaatcaa
     9961 ccatttgata cttccggttt tattactggc gtacttctcg ctcgccagta ttacgcgcct
    10021 gactcgttcc gcctgtcttg gcgaaatgaa taaagaatat attctgctgg cgcgcgccaa
    10081 aggagccagc gagatgacaa ttttgctacg tcatgtttta cccaacattc gcggcacctt
    10141 gctcactgtt atcgccctcg cctatacatc aatgctggaa ggcgcggtac tgacagaaac
    10201 cgtcttttca tggccaggta ttgggcgcta cctcactaca gcgctcttcg cgggtgatac
    10261 cacagcaatc atgggaggaa cgctgctgat tggcgtctgc tttgtgttga taaataactt
    10321 aaccgacctg ctggtccggc tgaccgatcc gaggttacgc tgatgccgat gtttctcttt
    10381 ttacgccgtt tacgccattc acccgccgcg ttttgcgggt tgataattat cctgttattg
    10441 atgctaattg cgctcttcgc gccgtggctg gcacctcacg atcctaactg gcaggatgcg
    10501 gcggcgcggt tacaagcacc gagtcgtcag cactggctcg gcactgacag ttacgggcga
    10561 gacctacttt cacgtttaat ctacggcagc cgcccggcac tggggctggt ggcattggtg
    10621 acaatcatta cactcccggt cggtctgctg attggcattt tagccgggta ctacggcggc
    10681 tggctggaac gcgtgctgat gcgttttact gacgtagtaa tgtcaatgcc gcgtttaatt
    10741 ctggcttttg cttttgttgc catgctgggg ccggggctgg ttaacggcgc actggccctg
    10801 gcattaacca cctggcctgc ttatgcgcgt caggcacgga gtgagatcca acgtctgcga
    10861 cacagtgatt atcttgccgc cgcagaaatg atgggcatcc gcggcaggcg tctgctggct
    10921 ggtcatattt taccattgtg cctgccatct gccattgtcc gtctggcgct ggatttagcg
    10981 ggtatcattc tggcggctgc tggcctgggt tttcttgggc ttggtgctcg cccgccaatg
    11041 gcggaatggg gagccatgat cgccgacggt atgcaggtca tttttgatca atggtggatt
    11101 gccgccgctc ccggcgcaat tatcttaatt gccagtctgg cctttaatct gctgggtgat
    11161 ggcttgcgcg atgttctgga gcctcaacat gactgactca cgtcttagta tcaacaatct
    11221 ctgcgtcgat tatccggagt cccgggtcgt caacaatgtc agttttacat taggcaatga
    11281 acgtctggcg ctggtcggtg agtcgggttc aggtaagtcc atgaccgccc gcgccctgat
    11341 ggggctggtg cgtaaaccgg gtgtagtaac cgcggacaca cttaacattc tggggcgtga
    11401 tgcactcacc cttaacgcgc gtggatggca acagttgcgc ggtaacgata tcgcgatggt
    11461 attgcaagat ccgcgttacg cgcttaatcc agtaaagaca gtaaaagcgc aactggaaga
    11521 ggcgcttacg ctccatcaac gtctgaaccg ccgcgagaaa gaagagaaaa taaacaccgc
    11581 aattcaggct gtcggcctca atacgcgagt actgcaaagc tatcctcgtg aactttctgg
    11641 cggcatgggg cagcgagtga tgattgcaat tgcgctcatc aacgatcccc gggtattgat
    11701 tgccgatgag ccaacatcag cactggatgt gcgcctgcgt aatcagattc tggaactgct
    11761 ggttacccag tgtgaacaac gtcagatggc aatgttgctg atcagtcacg acctgccatt
    11821 agttgcccaa ttttgccatc gggtgctggt gatgtatcag ggaaataagg tggatgaaat
    11881 gcacgctgct gcgctaccta cagcaacgca tccttacacg cgcacactgt ggacttgccg
    11941 cccaaatgca caaacttacg ggcagatgct gccgacgctg gacaggacgg caatgacgcc
    12001 ggagaaatac catgacgatt gttgaaatta atcacttaca ggtgactttt gctgagaaaa
    12061 ccgcagtttc tgccgccagt ttttcggtta atgccggaga gacatttagt ttaattggcg
    12121 aatcaggctg cggaaaatcc acgattttgc gcgtaattgc tggattacag cgcgactggc
    12181 acggtcaggt gtcactgttt gggcacacca tcataccagg aatgcgtttt cagggcgatc
    12241 tccgccgcaa tgtgcaaatg gtgtttcagg atccctatgc ctcgttgcat cccaatcata
    12301 ctctgtggcg aacgctggct gaaccactca aaattcgtgg cgaaagagaa atagaaaaac
    12361 gcgtccagac tgcactggaa caggttggct tgcctttcga caccgctcgt cgctacccac
    12421 accagctttc cggtggtcaa cggcaacgtg tggttattgc tcgcgccctg ttgttacgac
    12481 cacaacttct tctgctggat gaacccacct cggcgttaga tatgtcggta caagcggaaa
    12541 tccttaattt gttgaatcaa cttaagctgg aacaccaaat gacatatctg ctggtcagtc
    12601 acgatgccga tgtcatcgcg catatgtctg atcgagcggc attgatgtcc gagggaaaaa
    12661 ttcagcgatt ttttgatcgc gacgccatgg aaaaggggga acatcggatg gattaaaaaa
    12721 cctgacattg cattaattca ttctgctttc aggtcttttt acagttgttt ttttgtccac
    12781 ccgaacactt tatactgtct ctcccctacc aaacttcatt atcaaaatga aaaaaattat
    12841 cactttgatg ttgtttttga cattctttgc ccacgcgaat gactccgagc ctggcagcca
    12901 gtatttaaag gcagcagagg ccggagaccg acgcgcacaa tattttcttg ccgacagctg
    12961 gtttagctcc ggcgatttga gcaaagccga atattgggca cagaaagccg ccgacagcgg
    13021 tgatgccgat gcctgcgcgc tgttggcgca gatcaaaatc accaatccgg tcagtctgga
    13081 ctatccacaa gcaaaagtgc ttgccgagaa agcagcgcaa gcgggcagta aagaaggtga
    13141 agtaacgctg gcgcatattc tggtaaatac gcaagcgggt aaaccggatt atccaaaggc
    13201 gatttcgctg ttagaaaacg cctcggaaga tctggagaac gactctgccg tcgatgccca
    13261 aatgctgctt ggtttgattt acgccaacgg cgtgggcatt aaggccgacg atgacaaagc
    13321 aacctggtat ttcaaacgta gctccgcgat ttcccgaacc ggttattccg aatactgggc
    13381 gggaatgatg ttcttaaacg gtgaagaggg ttttatcgag aagaacaaac aaaaggcgct
    13441 gcactggttg aacctgagct gtatggaagg gtttgatacc gggtgtgaag agtttgaaaa
    13501 attaaccaac ggttaaggta ggcgcagcaa acgtcgcatc aggcgttgat gtcggatgcg
    13561 gcgtaaacgc cttatccgac ctacaaaaat cacgctacat caggctatta ctcagtcagg
    13621 catccacaca ttgccgggtg gatatccccc cggcaatctt caattactga tccgcagtct
    13681 tatcaaattt gcccagcact tcacgctcat aagccagcgc tttcttacgg tcaaatttgt
    13741 gttcccactt ggcaatcacc agcaccgcca gcgcattacc caccacgttc agcgcagtac
    13801 gcgccatatc gaggatacgg tcaacaccgg caataaacgc cagaccttcc agcggaatac
    13861 ctacgctacc cagcgttgcc agcagcacca caaacgacac gcccggcacg ccagcaatcc
    13921 ctttcgaggt caccatcagc gtcagcacca gaatgatttc ctgccagatg gagaggtcaa
    13981 tgccatacag ctgagcgatg aaaatagccg caatactttg atacagcgtc gaaccatcaa
    14041 ggttaaaaga gtaaccggtc ggcaccacga aactggtgat cgacgccggt gctccgtagg
    14101 cttccatctt ctcaataatt cgcggcagca cgctttcaga gctggcagtg gagtacgcca
    14161 gaatcagctc atctttcaga atacgaatca ggatccagac gcttaacccg cacaggcgcg
    14221 ccacaattcc cagcactacc agcgcgaaga acagaatggc gaaatgcacc agcagcacca
    14281 gtttcgccag tggccacaga gacgagaaac caaagttagc caccgtcacc gcaatcagcg
    14341 caaacacacc caccggcgca taacgcatca ccatgtgagt cactttaaac atggtttcag
    14401 agatggagcg gaaaactgtc accagcggtt cacgatgcgt cgcgggcagg gatgaaagcc
    14461 ccagaccaaa cagcaccgag aagaagatga tcggcagcat ttcgcctttc gccatcgacg
    14521 ccacgatgtt cgtcggcacc agcgacaaaa tcgtgcccat aatgccgtgg gaactgcttt
    14581 gtaccgcttc cgtggtgctc tgatatttcg agatatcgac ggtcgccagc tgcgacatat
    14641 ccaccccggc accgggctgg aagacgttcg ccagagtgat ccccaaaatg atggcgacgg
    14701 tggtgatcac ctcgaagtag ataatggttt tcgcaccaat acgcccgagc tgtttggcat
    14761 caccaacacc cgcgataccc accaccagcg tggagatcac aatcggcaca acaatcattt
    14821 tgatcagatg gatgaagata tcacccgccg gagagagcaa attgacgacc agccagtcgc
    14881 ggctgtcgct atggtagtgc aggtagcttc ccaggagaat gcccagcacc atagcaaaca
    14941 gaatctgcca ggccaggctg aattttatat ttttcatcag aataacttcc tcaatggagc
    15001 gccgttcccc gttcagaaac gcgagaacgt gacatactgc cagggcatga attgtgttgc
    15061 gttggtttgt agattttttt aacgcttatg atcagtatca tttgcgcggt atgttgcgca
    15121 accctttaac aacagagctt ttcgctgcat taatgcataa taacgcgagt ttatggcaga
    15181 attaatgaat aaccatttgt tatagaagga tttttatttg tttaatctgc aataatactg
    15241 attagtgatt atttttgatt atatgttcat tcgattattt tttatgcagc tcaataacat
    15301 aatcttcatc accagatgat taatctgatc tccgctgcaa caatttcgcc atattaatcg
    15361 actcgaccag ctgtgtccgg ttgatccggg gtgagttgag atccatcact ttttgccata
    15421 gttcgatggc ttgcgcgtaa ttcgcctgca taaacgcatc ggaagccagc agcatcagag
    15481 cggtgatttc gttagagtcc agcgcgaggg ctttgtcgat cattgcgcga gcctggacgg
    15541 tcatatgttg gctggcctgg taatacagca ccgtcgccag cgccgcatac agttcagcgt
    15601 tctcaccacg tagttgcaac gcctgacggt acgccagcag cgaattgctg taatcgtttt
    15661 gccacagata atactcgccc agtaacgccc actgttcgct gttttgtgga ttagcacgga
    15721 ttttatcctg caatgcctga agctgcgctt ccggggtttg ctggctggca aactgatgca
    15781 gcggatcgcg ctgacgctga tactccgcac gtaccgcctg ccattttgga cttaacagat
    15841 aactgccgac acagagaaat accataaaaa tcgtgagtgt tgtcagcatt ttcaccggta
    15901 tttggggctg tttcatcgct tcgccctcac tcgccagagg atcagtgcca tcagcagtaa
    15961 caacactact ggcagcgccc acaacaccag cgtctgaccc gttaacggtg ggttatagcg
    16021 aacaaaatct ccgtagcgtt cggtcatcca gccaatgatt tcgacttcac ttttcccctc
    16081 ttccaccatg gtgtaaacct gatggcgcat actgactgcc accggcgcat tggattccag
    16141 taagttctga ttctggcact gcggacaacg taactggctg gcaatattta acgcctgttc
    16201 ctgttgttgc ggattggcga attgccaggt gtctacaacc tgagcgcgcg caaaacaggt
    16261 aaataacagc agtagcgtga gaagcccttt attcatcgcg cttccttccc cgccatccgc
    16321 ttaacaatgc gcccgcaatc atcaacaatc ctccccccca gatccagcgc acaccgcttt
    16381 gtacatacaa acgaaaagcg taacgatccg ctccggtttt ctcgcccatg accgcatacc
    16441 agtcatggat gccattccag cgaattgacg gttccatcat ttgctgacga cgtgcttcgt
    16501 aaaaacgccg ctccggcgtt aattcaccaa tgcgttgctg atggtcaaac agtgccacta
    16561 tcgctttttc gctggtgtaa ttgcctttgg cttgcagatc gaggcgctca aaacggaagg
    16621 tgtatcctgc cagcgtcacc tgctgacccg gctgtaaatt caggctgatc tcctgacggc
    16681 tgacgctgga aaccacaatc cccgcggcaa ataacagcac gcccgcatgc gccagcagcg
    16741 ccggaagctg agcacgtttg ccagagacaa acgtcgccag cacaatcacc accagcatca
    16801 acagagcaaa cggtaacgtc gcgcggttaa aatacggcgc gccaacggag aggcgtcccc
    16861 agcccagcag gctgtaaatc atcggataaa gcgttcccac cagcacgatc agtagcactg
    16921 cgcaaaacag caacagcgta gcgaggatta acatttcccg cgataacccc gaaaaacgca
    16981 ccgccgggcc accgtcccgc gctcgccagc catacagagc cagagacgca aggctaatca
    17041 gtgcaaacag gctgaacaac ggcacggcgc ggacgttatc cagcgcgaac gcatgaaccg
    17101 aaaccagaat gccagaacgg acaattaagg tgcccagcag cgacagcatc agagttacta
    17161 tcgccagcaa cagcgaccag tggcggaaaa tcccccgctg gcgtgtcaaa gacaaactgt
    17221 gtagcaacgc agtagatgaa agccagggta ataacgacgc gttttcaacc ggatcccaaa
    17281 accaccaacc gccccagccc agttcgcaat aagcccacca ggaaccgaga atgatccctg
    17341 cggttaatgc gctccagcca ggtagtgccc aacgccagca aattcgggcg caggtaccat
    17401 caaactcgcc gcgcagtaaa ctcgccagcg ccacgctcgc cgccaccatc aaaccgccgt
    17461 agccgagata aagcagcggt ggatgaaaga taagaccggg atgttgcagc attgggttga
    17521 gatcgcgccc ttctattgcg ggcggaaata tcctcacaaa aggatcggac catccgacaa
    17581 caaacagcag cagtgcggca agtatcagcg ataaaaccgt taacgtgagc ggaaatagcg
    17641 gatcggtttg ctgtcgataa tgccaggcaa acagcgcgct ccaggcggaa agcagcagca
    17701 cccaaagcag cagcgaacct tcatgaccgc cccacaccgc cgccagtttg agttcccacg
    17761 acagcaggct atagctatgt tgggcgacat aaatgaccga gaaatcgctg atgagaaaac
    17821 aatacgtcag tacgccaaag gcgagcagca ggagcgcgaa ctgcgccaga atgccgatgc
    17881 aagtgagtcg catcatggca ggccagcgca accgcactcc cgcgaaggcc gtcaacgggg
    17941 tcaacacgtt gaccccgaga cttaacaaca acgccagaaa accgacttca ggaaggaata
    18001 cgtccagaaa ccacctctgt tctggcttac gccaccgtca actgcccggc gtagagaatg
    18061 aaaaagcgca gcatcaacac gccgaccagg ctggccccac acgccgccag cacggcagga
    18121 atgccggaac tgcgattgac ccacggtttg agcaacattg gcacaatcag ccccagcccg
    18181 gcgacaccaa gccagaacca ccacgtccag aaaccgccgc ccaatgccgc caccagcgca
    18241 cgcactttac cgtcatcgcc cagcgccaga ccgacaaaaa acgccaccag caagaagatt
    18301 tcaccccata ccacggggat ttccatacgg tgtacaaagt gcgcttccgt ggaatgcggg
    18361 ttactgcgtt ggcgtatcgc catggcgatc agcgccaccg ccgcaccaga cgagatgccg
    18421 gagaagagga acagcaccgg caggatcggg ttattgagga acggatacga tttcagcgcc
    18481 gacagcagaa agccggtata agcccccaac aacaccgcca gcaccagcat caatgtttcc
    18541 agtccgcgat gaacgggcgc cagcaggctc aagacctttt gcacgatccc cagcttcggc
    18601 aaccagcgtt gttgcagggc aagcaaatca tgttcaaaga ttttcgccag ccacagcacc
    18661 agcaccacca tgtaaagctg aaacagcatc acccccatcg acatcaccga ggtaaaactg
    18721 tagtggaaca tcagcttcca gaaggtccac ggtcttgtca ggtggaagac gaggatcaac
    18781 agaccgagga tcaccgcgcc aggcccgaca atcagcgtgg tgcgcagcaa cgtactgtct
    18841 gcaccgcccg cctgcgggta gacacgacgt aacagcacgg ccagcgtcac cagaccggca
    18901 gaaataccaa tcaaaaacag gtagatggca atcggccagt cccacaccag cgattcaaaa
    18961 tgaaatgcgg aagtctgcgt cattggctca cctccccgta tttaaacggt acgcggtaca
    19021 gcttcggttt ggttcccagc gccagcttgt agcggtaagt gggcttctgg cgcagcagtt
    19081 gcgaaatctc actgctggga tcgtccagat tgccaaacgt cagcgccttc gtcgggcaag
    19141 cctcaacgca cgcgggcagc ttaccggcct gcaaatgggt cttacggcag aaatcgcatt
    19201 tgtccgccgt cttcgtgacc ggatggataa agcgcacgcg gtacggacag gcggcgatgc
    19261 agtactgaca accgacgcag agatccggat taacatcgac aatcccactg gcagcgtcgc
    19321 gaaaagacgc accggtcggg caaacgtcaa cgcacggcgc atgatcgcag tgctggcaag
    19381 acttacggaa gaagcgatat ttcacatcag gaaattcgcc ctgcggctcg ctacgaataa
    19441 tcgtcaggcg cgagacgcct tccggcacct tgttcacttc ccgacaagca tccatacagg
    19501 cggtacagcc gatgcataac gactcgtcat gcaccatgcc gtaacgcacc ccattgatat
    19561 tcaacgtttt cgccaccacg cgtcctgcgg tgccgctgac ggctgccagc acgccgacgc
    19621 cggtgagaaa ctggcgacga gaccaggtca tggctgctcc ttaagcaacg gaacagacgc
    19681 cgggttaaag ttcggattgg tgcgctgatc gctgtggcaa tcgacgcaaa tcttaatccg
    19741 tcctttgtca cttaacgtct gcatcgtatc ttgctgtgga tgcagggaat ggcagctggc
    19801 gcacgccact ttggttacgt ggacatcgtg cggccagaac gctttttgca actgttccgg
    19861 cagatggcag gacatacaga cgctgttctg ctctccaacc ttgtacatcg gctcgttaaa
    19921 gcgcatcaca tctttcaccc cttcgcggtg ttgcggtgat ggctgaccgt ggcagttggt
    19981 gcaggtgacc ggcagtttgt tattcgggtt gataacagaa gcatgtttgc catgcatacc
    20041 ttcggtatct ggcttatgac agtccagaca ggcggcatcc gggttacgct gctgggtaac
    20101 ttcgtagcga tcatcagatg cctgcgccgc aggggtagcg gtaattccgt tcaggctcca
    20161 caacaggccc gacgccagca ccccggcagt taataacgaa cgtaatacgc tcatattcac
    20221 tccattgtgt tttccgcccc ttgcgaggcg gaacggggtt attggcttaa cagaccgttt
    20281 ttacgtgcct gctcttccca ctgcgggatc accgttttga tgaagtcctg cttctcggcc
    20341 ttgatttgct ccatgttcag gccaatggcc tgctgggctt tctctttggt cgagatatcc
    20401 gggatctgga tttcatgggt aatgcctttg gtcgccagca ggcgcgccag tttggtgcgt
    20461 gcatccgccg ctttatccat cgccgtaccg agcatcctca gaccttcttc cggtgcgtgc
    20521 atatgaatgc cgtgggaagc gatcgccaaa tcccaacgcc actgggcatg acggatatcg
    20581 tcctgaatcg gcttcatttc ggcttccgtt gcacctgcat ccagcgccgc tttcgcttcg
    20641 aagtgagcgt gaaccagttg atcttcaacc ttgattttca ggtcgttaat cgactgctta
    20701 cgttccgcga ccactttctg caaggcagct ttgtcctggg tatggcagtt cgcacaagtc
    20761 tgggcgaagt tatcaaacgg attaccaatt ttatggtcgg tgtagagttt gccttcggcg
    20821 ttctgcactt ttggcatatg gcaatcgata caggtcacgt tgtttttgcc gtgaatgccc
    20881 gctgtccagg tttcatattc cgggtgctgc gctttcagca ttggcgtttt cgacagggag
    20941 ttagtccagt cagagaaggc aattttgtcg taatactgct ccatattttc gactttcatg
    21001 ccgtcatccc acgggaattt aaccgctttg tttttgccgt cgaagtaata ctccacatgg
    21061 cactgaccgc aaaccatcga ttgctggtcg aaacgtccgg ctttctcgaa aggtttacca
    21121 atggcttcca tcgcgcgagc cgcatacgga cgggaaaggg ttaactccgg tttgcctttg
    21181 gcgaactctg gtgaggcggt gttatggcaa tcggcacaac ctaagttgtt gacgatttcc
    21241 gggccgccgc gcgcccattt accgtggaag tagccatctt cgccgtcttt ctggatcaga
    21301 cgcgccacat ccgggctttt acaactccag cacgccatcg gtagcggacc atcttcagcg
    21361 tttttcggcg caccggtacg caaggtttca cgcacatcgg tcacagcaaa ggcatgtcca
    21421 cgcggcttgt tgtaatcgcg cgagaaggga taccctgccc acaggatcac cagccgtgga
    21481 tcttccgcca gggcgtcaac acgctctgac tgttccgagg tagctttcca ggagagatat
    21541 tgatcgggat gctgcggggc aaaggtttca ttcttcgctt ccacagttac aggtgttgcg
    21601 ggagcagccg tttgttcagc gtgaacagaa gtgaaaaaga aaaaaggaat caataagctg
    21661 aagatacggc gtgcgtttat ttttatcctt gtcatagggc ttcatccgaa ttgcgccatt
    21721 gttgcaatgg cggtttttat tgtttttcac gacagtaacc gcacctacac tgtcatgaca
    21781 ttgctcgtcc ctatgtgtaa caaataacca cactatgaat gttgtcttta atcaattgta
    21841 agtgcatgta aaataccact ttagagttag tcagtatctt cctctttttc aacagcatgc
    21901 ataactgcat gttcctcaaa gaattaatca acttttgttg ctgaccttca aaaattaccc
    21961 tgccgtttat ttgcacaatt ctacttttgc gtgatctgtc gcccaaatac taaacaaaac
    22021 tgccaatacc cctacattta acgcttatgc cacatattat taacatctta caaggagaac
    22081 aaaagcatga gtcaaattca caaacacacc attcctgcca acatcgcaga ccgttgcctg
    22141 ataaaccctc agcagtacga ggcgatgtat caacaatcta ttaacgcacc agataccttc
    22201 tggggcgaac agggaaaaat tctcgactgg atcacaccgt accagaaggt gaaaaacacc
    22261 tcctttgccc ccggtaatgt gtccattaaa tggtacgagg acggcacgct gaatctggcg
    22321 gcaaactgcc ttgaccgcca tctgcaagaa aacggcgatc gtaccgccat catctgggaa
    22381 ggcgacgacg ccagccagag caaacatatt agctataaag agctgcaccg cgacgtctgc
    22441 cgcttcgcca ataccctgct cgaactgggc attaaaaaag gtgacgtaat tgcgatttat
    22501 atgccgatgg taccggaagc cgcggtcgcg atgctggcct gcgcccgcat tggcgcggta
    22561 cattcggtga ttttcggcgg cttctcgccg gaagccgttg ccggacgcat tatcaactcc
    22621 agttcacgac tggtgatcac ttccgacgaa ggtgtgcgtg ccgggcgtag tattccgctg
    22681 aagaaaaacg ttgatgacgc gctgaaaaac ccgaacgtca ccagcgtaga gcatgtggtg
    22741 gtactgaagc gtactggtgg aaaaattgac tggcaggaag ggcgcgacct gtggtggcac
    22801 gacctggttg agcaagccag cgctcagcac caggcggaag agatgaacgc cgaagatccg
    22861 ctgtttattc tctatacctc cggttctacc ggtaagccaa aaggcgtgct gcacactacc
    22921 ggcggttatc tggtgtacgc ggcgctgacc tttaaatatg tctttgatta tcatccgggc
    22981 gatatctact ggtgcaccgc cgatgtgggc tgggttaccg gacacagtta tttgctgtat
    23041 ggcccgctgg cctgcggcgc gaccacgctg atgtttgaag gcgtaccgaa ctggccgacg
    23101 cctgcccgta tggcgcaggt ggtggacaag catcaggtca atattctcta taccgcgccc
    23161 acggcgattc gcgcgctgat ggcggaaggc gataaagcga tcgaaggcac cgaccgttcg
    23221 tcgctgcgca ttctcggttc cgtgggcgag ccaattaacc cggaagcgtg ggagtggtac
    23281 tggaagaaga tcggcaacga gaaatgcccg gtggtcgaca cctggtggca gaccgaaact
    23341 ggcggtttca tgatcacgcc actgcctggc gctaccgagc tgaaagccgg ttcggcaaca
    23401 cgtccgttct tcggcgtgca accagcgctg gtcgataacg aaggtaaccc gctggagggg
    23461 gtcaccgaag gcagtctagt gatcaccgac tcctggccgg gtcaggcgcg tacgctgttt
    23521 ggcgatcacg aacgttttga gcagacctac ttctccacct tcaaaaatat gtatttcagc
    23581 ggcgacggcg cgcgtcgcga tgaagatggc tattactgga tcaccgggcg cgtggacgac
    23641 gtgctgaacg tctctggtca ccgtctgggg acggcggaga ttgagtcggc gctggtggcc
    23701 catccgaaga ttgccgaagc cgctgtcgtc ggtattccgc acaatattaa aggtcaggcg
    23761 atctacgcct acgtcacgct taatcacggg gaggaaccgt caccagaact gtacgcagaa
    23821 gtccgcaact gggtgcgtaa agagattggc ccgctggcga cgccagacgt gctgcactgg
    23881 accgactccc tgcctaaaac ccgctccggc aaaattatgc gccgtattct gcgcaaaatt
    23941 gcggcgggcg ataccagcaa cctgggcgat acctcgacgc ttgccgatcc tggcgtagtc
    24001 gagaagctgc ttgaagagaa gcaggccatc gcgatgccat cgtaacccac aattgccgga
    24061 tgcggcgtat aacgccttat ccggcctaca ttcggcaagg gttacccaag cgttaacctt
    24121 ctcccataag ggagcgggaa ttaaaacaat ccctacatta cctctggaga atctgtgatg
    24181 aatggtacga tttatcagcg gatagaagac aatgcgcatt tcagggagtt agtcgaaaaa
    24241 cggcaacggt ttgccaccat cctgtcgatt attatgctgg cagtttatat cggctttatt
    24301 ttactgatcg ccttcgcgcc cggctggctg ggcaccccgc tgaatccgaa caccagcgtc
    24361 acacgcggta ttccagtcgg tgtcggggta attgtgatct cctttgttct cactggtatc
    24421 tacatctggc gggcgaacgg cgaattcgac cgtcttaata acgaagtcct gcatgaggta
    24481 caagcatcat gaaaagagtt ctgacggcgc ttgccgccac actccctttc gcagctaacg
    24541 ccgcggatgc tattagcggg gccgtagagc gtcagccaac gaactggcag gcgattatta
    24601 tgttcctgat tttcgtcgtg tttacgctcg gcattaccta ctgggcgtca aaacgcgtac
    24661 gttcgcgtaa tgactactac accgcaggcg gcaatatcac cggttttcag aacgggctgg
    24721 cgattgccgg ggactatatg tccgccgcct cattcttggg gatctccgcg ctggtgttta
    24781 cctccggcta tgacggctta atttactcgc taggcttcct ggtaggctgg ccgatcattt
    24841 tgttcctgat cgccgaacgt ctgcgtaacc tcggtcgcta cacctttgcc gatgtggcct
    24901 cttaccgtct gaaacaaggg ccgattcgta ttctttcagc ctgtggttct ctggtggtgg
    24961 tggcgcttta ccttatcgcc cagatggtgg gcgcaggtaa actgatcgag ctgctgtttg
    25021 gccttaacta tcacattgcg gtagtgctgg tcggcgtgct gatgatgatg tacgtcctgt
    25081 tcggcggcat gctggcgacc acctgggtac aaattattaa agccgtgctg ttgctgttcg
    25141 gtgccagctt tatggccttt atggtgatga aacacgtcgg ctttagcttc aacaatctgt
    25201 tcagcgaagc gatggcggta cacccgaaag gtgtcgacat catgaagccg ggcgggctgg
    25261 tgaaagatcc tatctccgcg ctctctttgg ggctaggact gatgtttggt acggcgggct
    25321 tgccgcatat tctgatgcgc ttctttacgg tcagcgatgc ccgcgaagca cgtaagagcg
    25381 tgttctacgc caccggattt atgggctact tctacatcct gacctttatt atcggctttg
    25441 gcgcgatcat gctggtcggt gcgaatccgg aatataaaga cgcggcgggc catctgattg
    25501 gtggtaacaa catggcagca gttcacctgg ccaatgcggt gggcggcaac ctgttcctcg
    25561 gctttatttc agcggttgct ttcgccacca ttcttgcggt ggttgcgggt ctgacgctgg
    25621 cgggcgcatc ggcggtgtct cacgacctgt acgctaacgt cttcaaaaaa ggtgcgaccg
    25681 aacgtgaaga gctgcgggta tcgaaaatca ccgtactgat cctcggtgtg attgccatta
    25741 tcctcggcgt gctgtttgag aatcagaaca tcgcctttat ggtggggctg gcgtttgcca
    25801 tcgcggcgag ctgtaacttc ccgatcattc tgctttccat gtactggtcg aaactgacta
    25861 cccgtggcgc aatgctgggc ggctggttag gactgattac tgcggtggtg ctgatgatcc
    25921 tcggcccgac tatttgggta cagatcctcg gtcacgaaaa agccatcttc ccgtatgaat
    25981 acccggcgct gttctctatc agcgtggcat tcctcggtat ctggttattc tcggcaacgg
    26041 ataattcagc ggaaggtgcg cgcgagcgtg aactgttccg tgcgcagttt atccgttccc
    26101 agactggctt tggcgttgag cagggccgcg cgcattaatc tctccccttc cccggtcgct
    26161 tgatcgggga atttcttcag aacatcatcc tggccaccag cggcgaaacc aataccatca
    26221 atacacctga aagcatcatg accaggcttg caactacgcc ttcttgttga cccagttcat
    26281 acgaccgcgc agtcccggca ccatgagatg ccgcgccgaa tccagcacct ttcgccatcc
    26341 cttcgcgaat agagagacgt aaaaacaaca cgtcgccaat cgccatgcca aacacaccgg
    26401 tgaccaccac aaacagcgcc accagatcag gctgtccgcc gagcgactgg ctcgctgcca
    26461 aagcaaatgg cgtagtgacc gaacgcaccg ccaggctacg ctggatctca tctggcaggg
    26521 taaacaatcg cgccagccac actgagctgg tcactgccac caccgttgcc gtcagtacgc
    26581 ctgccgtcaa cgacatccag tgacgcttaa tgatcgcccg attgtcatac actggtaccg
    26641 caaaggcgat ggtggccgga cctaatagcc atagtaacca gtgcgcttcg ccaatatagt
    26701 tttgccagga aatattgcca aaaaccagca gcataaccag tagcgtaggc gtcagcacca
    26761 gaggcatcaa tggcaattta cgaaagcggc gataaaggcg tttgttaaga aaatagataa
    26821 ctagcgtaat tgccagacag agcatactta gctgaaaatt agccacggtt taacctgcgc
    26881 atttcatagc gatagacttt ttccactacc caggcagtcg tgcccagcac catcaccgta
    26941 ctcagcgcga tcacagcaaa aatacgccag ccatccacca gtaatagttg tgcatagttc
    27001 accacagcca ccacggcagg gacaaaaaac agcaacattt cggccaatag ccaacgcgcc
    27061 ccggctcgaa cccattgcag aggaagaatg cgacaaacaa tcagcgccag catcaggatc
    27121 atccccacca ggttggcagg cagcggcaaa tgcagccagg agacgagata ctgcgcaaaa
    27181 ataaataaac ctgcatacag cacaacctga aaaaatacct ggatagattg cactacggca
    27241 ggggtaatgc ggcgaagcgc cacgggcatg agaggatatc ctgaaaataa aacagaatgg
    27301 taagtataga gagcgctcgc ccctgactga aatgaattaa aatcattgca ggtatagtca
    27361 aagggaatag ttatggatat cagaacgctg cgttattttg tcgaagtcgt gcgccagcaa
    27421 agcttcaccc gcgcagcgga gaagttgttt gtcacccaac caactatcag caagatgctg
    27481 aagaatctcg aagatgagct gaactgtata ttgttgatcc gcgatggtcg tcggctgctt
    27541 ctcactgata ctggtcgcgt cgtatttgag cgcgggctgg ctttactggc tgagtttcgg
    27601 caactggaag ctgagcttag cgatatcaat cagcttaaaa ccggcgtttt gcggctggga
    27661 atcccgccga tggttgggat gttgatggcc gggccgattg gcctttttcg ccagcgttac
    27721 cccggcgtag aattgaaaat ttcggagttt ggtggtctga cggtacaaca agcggtgatg
    27781 aacggtgaac tggatatggc actgacagcc ctgccagtag aagaaagcgg cctgacaacg
    27841 ttatcgctgt ttagtcatcc actatgtgta ctgacgccac attcaggtaa atggcttgaa
    27901 tgtgatgccg tctcaccgga gacattggcg gaacatcctc tacttatcta taacgaagat
    27961 tttgccttaa gtaagcaatt gatgaagtta ttcgatgaac atggcgttaa gccgagaatt
    28021 gcagttcgca gcgggcaatg ggattttctc gccgcgatgg ttcaggccgg tgtcggaata
    28081 gccatattgc cggaacccat ttgccagcga ttagataaaa acaccttaaa atggctaccg
    28141 ctggaaagcg aactaagctg gaaattagga atgatttggc gtgagggcgt ttacctttcc
    28201 cactctgccc acgcgtggct gagctgttgt gaaggatact ggttaacgcc agagtaagtc
    28261 gggttggcgg gctcgcccgc caaccatgca gattactgat tttcctcaat cagcaacgct
    28321 tcaagcaaat cgagatcgtg cagtaatttt tgcagcgttt cgttactgat ctcccgcgtg
    28381 gcgcgcaggt ggtacagttc agcacgttca gaacgcaatg ccgccagacg gaagcgacgc
    28441 tccaagttct cttccaggat tgaactttct acatcattac gtccatcggc gcgacgacgc
    28501 aggttaccaa tgacgcgaga actaacctct gtgagcagct ggttatcgat attctcttcg
    28561 gtatccgccg ccaaacgctc ctccattttc tggatcgcca caatcgccac ttctgccgtt
    28621 gccgctcgtg caatacgttc ctctttcaat tgttgcgaat ggtccgcgac ttctatgtgt
    28681 tgtagcagaa taggcaacat caccacgccg acaaacaacg agaagagaat gacgccagcc
    28741 gccaagaaca ccaactcata gcgcgcaggg aagacgttac catccggcaa gagaagcggg
    28801 atagagagca caccggccag agtaatcgcc ccacgcaccc cggcgaaaga cgcgatcagg
    28861 atttctcgtg tagtccacga accaaactcc atcggcttct tcttcaggaa gcggttgctg
    28921 aactttttca tcgtccacag ccagccgaaa cggaccagca tcagcgccgc atatatcaga
    28981 ataatatcgg taaacagcat ccagatttcg acgttagggt cgatttctgc cgccatcagc
    29041 gacgtctcca gaatccccgg caactgcaga cctaacagca ggaacaccat gccgttaaag
    29101 acaaattcca gcatcgccca ggtgctgttt gcgcgcaggc gcattgccag cggcgcacgg
    29161 cgcatcacac cggagcgggt gatggtcatc ccggcggcaa ccgcagcgag gatgccggaa
    29221 acgccaatat gttccgcaat cagataagaa gcgaatggca gcaagaacag cagaacgatc
    29281 tgcgtcgcgg gttcatcacc gccccagcgg ctgaggaatc gcagcgagcg accatacagc
    29341 cagctcacca caaaaccggc gagaataccg cctattgcca ctttcataaa ttcgaccgtc
    29401 gcaccgccga cggtgaagat cattgtcccc atcgccactg ccacggcaaa cttcaacgac
    29461 accaggccgg aggcgtcatt catcaacgct tcgccctgca aaatgcccat gatttttttc
    29521 gggatgcgcc cctcgccaac aatcccggag agcgctacag catcggtcgg agaaagcacc
    29581 gcagccagcg caaaggcggg gatcagcgga atgcccggca ccacccagta aataaggaag
    29641 ccaatgccga ccacggtgac caccaccagc gccagcgcga ggccgaaaat ctctcgccca
    29701 tgttcgagaa attcacgggt cggcgttttc cagccatcag cgaacagcaa cggcgggata
    29761 aacaagacta aaaagagttc aggatcaaac tccacatgca aaccaaacgt cggccacgcc
    29821 agtagcgcac cgatggcgat ttgcataagc ggaagcggga tctgaaaggg catgacacga
    29881 gtgaccaccc cggacagcga gaccacgagg gtcattatca gtatggtgaa gaagatttcc
    29941 atgcgttccc tgtttgcgat gttttttatg atctgagaaa ggcgttatgc ctgaattctc
    30001 attttttcag aatatcgcaa aagctcacac aatgtgcgga gaaaataaaa acgggcagcc
    30061 attgccaccc gttttcagaa tcctatgtaa gattagatag cccagccacc cgcatagaag
    30121 gtcaccagcg ccacggcaat aacgaccgta ccgatgttca acttgcgcca ttcgccggaa
    30181 accagacgac caatcaccag agtcgcgaag ccaatcatga tgcctgttac gatgttacag
    30241 gtcagcacga tgaataccgc cgtaaccaga cccgccatcg catcaacaaa atcagcaaag
    30301 tcgattttcg ccacgttgct cagcatcagc aggccaacgt acatcagcgc cggagccgtt
    30361 gcgtaccccg gaacgaggta agagagcgga gagaggaaca gaatcagcag gaacagcacg
    30421 ccaacggtga tagccgtcaa accggtttta ccgcccgccg ccgtacccgc cgcagactcg
    30481 atgtataccg ctgccggagc tgcacccacg aggccagaaa aaacgctgct catggagtcg
    30541 gtggtcagtg ctttcccgcc gtcgatgatc tgcccatctt tatccagcag gttcgcctga
    30601 ccggcgacgg cacggatagt tccggtggca tcaaatactg ctgtcatcac cagtgccaga
    30661 acacttggca ggactacagg attcagcgcg cccataatgt ccaggctgcc aatcagtgaa
    30721 ttgccgtttt catcgctcaa tgaaggcatg gcaaaaacgc cggagaaatg gacgttagga
    30781 tcgaagatca aaccgacaat tgagataccg ataatggtca gcagaatgcc accagggact
    30841 ttcagttttt ccaggccgat aatcactgcc agacccacca acgacatcat caccggaaag
    30901 gtcgtgaaat cccccagcgc gacgggcaga ccatcaagtg ggtttttaat caccagaccg
    30961 acaccattag cagcaatcag cagcaggaac agaccgatac caatccccgt gccgtgcgcc
    31021 acaccgtgcg gcaagttgcg caaaatccag ctacggatac ccgtggcaga aattaccgta
    31081 aacagcacac ccatcaggaa cacggcaccc agcgcgacag gtacgctaat atgttgcccc
    31141 agcaccaaac tgaatgcggt aaacgccgtc agagagatgg cgcaaccaat cgccaacggc
    31201 agattagccc acagacccat cacgatagaa ccgagtccgg caaccagaca ggttgcaacg
    31261 aaaactgccg caggcgggaa gcccgcttta cccaacatgc ctggaacgac gataactgag
    31321 tagaccatcg ccagaaacgt tgttaacccg gcaaccactt cctgacggac agtgcttcca
    31381 cgttgtgaaa ttttaaacca ggcgtcgagt gaaccgccgg tacgcgctga tggcgtagac
    31441 ataagaaaac atcccctgag agtttaattt tcgtcaagat gcgtggcgga caatcccctg
    31501 ccagcgaaac gttatcttcc tgctccaggt ttgcgacaat ttgctgcgtc gcaaaaaaag
    31561 caaacgttta gctccgcgca tacaaaacgg gtggtaaaaa aagcaaacga ttatccggtg
    31621 atcatagcgg ctttttcaac cgaagtttgc tgcttttttc tctatttatt gtcttgacga
    31681 tgaaaaacca ggcaattgat gcgcaaacgt tatcgtcgat gcgcaagatt gcagcgttat
    31741 cgagccgtat accgttcagg attgcgataa aaaatcgtct gagatgacaa aaatattgcg
    31801 gctaaaaagt gtacttttac actggcgaag aaacgccggt aacatacaat taaagcatca
    31861 acaccaaccg gaacctccac cacgtgctcg aatgaggtgt gttgacgtcg ggggaaaccc
    31921 tcctgtgtac cagcgggata gagagaaaga caaagaccgg aaaacaaact aaagcgccct
    31981 tgtggcgctt tagttttgtt catcttccag taagcgtgcg ccggtacctt cttctcctaa
    32041 gcggtcgccc gggttacgca acgggcaatc actgcgcgaa aggcagccac aaccaataca
    32101 tccatctagt tcgtcacgca gcgccactaa ggtatgaatg cgccgatcca actcttctcg
    32161 ccattgggac gaaagctgtt tccactcttt cgcacttaac gtatgccctt ctggcaacac
    32221 gccaaacgct tcacgaatgg ttgcgagcgg aatgccaata cgctgagcaa ttttgataat
    32281 tgcgacatat cgcaacacat cacgtttata tcgccgctga ttgccgctat tacggatact
    32341 ggtaatcaac cctttgcttt catagaaatg cagcgccgat accgccacac cgctgcgttt
    32401 cgccacttcg ccgggggtta gcagcgcttt aatgcggggt aatttctttt ccataaatcg
    32461 ctttacctca agttaacttg aggaattata ctccccaaca gatgaattaa cgaactgaac
    32521 actgaaaaga ggcagattta tgtcccatca gaaaattatt caggatctta tcgcatggat
    32581 tgacgagcat attgaccagc cgcttaacat tgatgtcgtc gcaaaaaaat ctggctattc
    32641 aaagtggtac ttgcaacgga tgttccgcac ggtgacgcat cagacgcttg gcgattacat
    32701 tcgccagcgc cgtctgttac tggccgccgt tgagttgcgc accaccgagc gtccgatttt
    32761 tgatatcgca atggacctgg gttatgtctc tcagcagacc ttctcccgcg ttttccgtcg
    32821 gcagtttgat cgcactccca gcgattatcg ccaccgcctg tagttttatt gcccgcgcgt
    32881 taactcccgc gcgggtaatt accccatcca ttgcataaac acctgcggcg gcatcgcctt
    32941 cgcaaaaaac catccctggc aatagcgcac gccgcgtttg cgcagccagt taacctgcgc
    33001 ctcagtttcg acaccttcag cgatcgtttt taaccccagg ctgtgcgcca gctcgatgat
    33061 gtgttccgca atcaaatgac tggttttgtg ggtggtcagc gtctcaacaa acgatttatc
    33121 gattttcaaa atatcgacat tcaatgattt aaggttatgc aagttagagt agccaatacc
    33181 aaaatcatca attgccactt cgtaaccggc cttgcggaaa gccagaataa tcggcgtcat
    33241 tttgtcgaca tcaagaaatg catgttcagt cacttcaaat ttaatttgct gcgggcgcac
    33301 cgcatgttgc tctgttttct gattggttcg cgctatcaac cgtgaggtat gaaaatcgga
    33361 ggctgacagg ttaatagaaa catagcgatc ggtatgtgtt gccagatacg cgcccaaatc
    33421 gcgaaagaca ttatcaataa cataatcagt tacctgttcg atcatcccct ccttttctgc
    33481 cagcggaata aactctgccg gattcattac ttgcccctgc tcaccaggcc aacgtaacaa
    33541 cgcttcagca ccgatacatt tttctgtttt gatatcgatt attggctggt aataaaggca
    33601 aagctgatgt ttttcgaggg cgcgttgcaa tttgcgtttg ggagacaaat agttttgtcg
    33661 aatacgtagc cagagtaata gcagaacaag actccccaaa ataccggcgg gcaacgcaaa
    33721 aataagatga ttataaaaat gggttataag acgttgatat gaagtcgcaa caatggctgc
    33781 aattgggcgt tttgtcgaat aaactgtcgc atataaatag ccatttcgtt gtacggttaa
    33841 atcattcaaa tgaatcagcg gagaaaacgt tgctgccgag gcctcgttgc ttaacgagaa
    33901 aaaggttttc gtcaccgtat catacactcc ccattgcaat gtcggatcat cagacatcac
    33961 ttcactccag aagagagggt tgataaccgc cacataattt ccccgctgca tgtaggtcat
    34021 tttatagcca gagaaaaaag gcgtatcacg gtaataatag atagaaacgt taggttcgcg
    34081 cttataatcg gccggtgcaa tcgtatagcc atttacagac gctatcagcg atgagcataa
    34141 aaaatggtta tcacgagcat aaatcaattc attaatataa agatagccac gaataatatt
    34201 caacattcgc ttttgatggg ctggagtgca aacttgcccc tgatagcgtt cagctgcatc
    34261 gctcactaaa tctgcctgac gaatgaccag ctcagattta tccaacgcca gctgagcaaa
    34321 agttcggagc tgattattca cttctgattt tgcccaaaag aaggcaatcc atagcgacat
    34381 aatgatggga aagagaacta aaaagataat gcccggaaac gccagtaatt ggtgtcgtgc
    34441 acgatgactc attgctatca attccctgcc tgtttatacc cggttattgc aaggtgaaac
    34501 cctggctacc gacgtcttta gataaatctt acggtataaa tagcactttt gcctgatata
    34561 aaattgagac ctttttaatc gtcaccagga tgacgataat tcattgatct taaaaattaa
    34621 tcaccttcag gaatgcagag gcggcgggaa gatataccct taatgccgct gaaaaaacac
    34681 ataatctgta cggcaataaa agtcacggcc taatctggct aacaaacaac caattgtgat
    34741 atagttcaca aaattcatga aacaaacaga gtgtttcatt tttgtgttct gcaagccccg
    34801 cgatagccag agtgtatcac gcctcccgtg aacaacgccg cgctgtccag ggcatcagct
    34861 cttttccgag gaattgtttg atggctaccc tcaccactgg cgtggttctt cttcgctggc
    34921 aacttcttag tgccgtaatg atgtttctgg ctagcacgct caacatccgt tttcgtcggg
    34981 ctgattatgt cgggcttgca gtgatcagca gcggtctagg cgtagtttcc gcctgctggt
    35041 tcgcaatggg gttgcttggc atcacaatgg cggatatcac cgccatctgg cacaacatcg
    35101 agtcggtgat ggtagaagag atgaatcaga caccgccaca atggccaatg attttgactt
    35161 gatatgtaga agcctccata acggaggctt cttttttatg gttggcagat gctttaatca
    35221 tccaccttaa aacaatataa cctattgttt taatgacaaa tcagaacgga atatcgtcat
    35281 caaagtccat cggcggctcg ttagacggtg ctgccggagt agactgctgc gggcgagact
    35341 gcgcgccgcc gctgaactga ttgccgccct gcggctgctg aggctgaccc caaccgccct
    35401 gcggctgacc accaccgata ttgccacctg caggagcgcc accaccctga cgaccaccca
    35461 acatctgcat ggtgccgcca acgttcacca cgacttctgt ggtgtagcga tcctgaccgg
    35521 attgatcggt ccatttgcgg gtacgcagct gaccttcgat ataaacctga gaacctttac
    35581 gcagatattc gctggctact tctgccagtt tgccgaacag cacaacgcgg tgccattcag
    35641 tctgctcttt catctcgccg gtcgctttgt cacgccagga ttcggaagta gccagcgtaa
    35701 tgttggcaac tgcgccacca tttggcatgt agcgtacttc cgggtcctga cccagattac
    35761 caacgagaat aaccttgttt acgcctctgc tggccatgtt cgtgtctcct gaaaaaaaat
    35821 cgttctgaat aagtgtaaac gcgcgattgt accattacca atagcgcttt tactatgttg
    35881 tgacctcggt tccgggaaac aaacctggcc agacattgtt acacaacact ccgggtaatg
    35941 cattccaata ctgtatattc attcaggtca atttgtgtca taattaaccg tttgtgatcg
    36001 ccggtagcac catgccaccg ggcaaaaaag cgtttaatcc gggaaaggtg aatggataag
    36061 atcgaagttc ggggcgcccg cacccataat ctcaaaaaca tcaacctcgt tatcccccgc
    36121 gacaagctca ttgtcgtgac cgggctttcg ggttctggca aatcctcgct cgctttcgac
    36181 accttatatg ccgaagggca gcgccgttac gttgaatccc tttccgccta cgcgcggcag
    36241 tttctgtcac tgatggaaaa gccggacgtc gaccatattg aagggctttc tcctgccatc
    36301 tcaattgagc agaaatcgac gtctcataac ccgcgctcta cggtggggac aatcaccgaa
    36361 atccacgact atttgcgtct gctgtacgct cgcgttggtg agccgcgctg cccggaccac
    36421 gatgtaccgc tggcggcgca aaccgtcagt cagatggtgg ataacgtgtt gtcccagccg
    36481 gaaggcaagc gtctgatgct gctcgcgcca atcattaaag agcgcaaagg cgaacacacc
    36541 aaaacgctgg agaacctggc aagccagggt tacatccgtg ctcgtattga tggcgaagtc
    36601 tgcgatcttt ccgatccgcc gaaactggaa ttgcaaaaga aacataccat tgaagtggtg
    36661 gttgatcgct tcaaagtgcg tgacgatctt acccaacgtc ttgccgagtc gtttgaaacc
    36721 gcgctggagc tttccggtgg taccgcggta gtggcggata tggacgaccc gaaagcggaa
    36781 gagctgctgt tctccgccaa ctttgcctgc ccgatttgcg gttacagtat gcgcgagctg
    36841 gaacctcgcc tgttttcatt taacaacccg gcgggagcct gcccgacctg tgacggtctt
    36901 ggcgtacagc aatatttcga tcctgaccgc gtgatccaga atccggaatt atcgctggct
    36961 ggcggtgcga tccgtggctg ggatcgccgc aacttctatt atttccagat gctgaaatcg
    37021 ctggcagatc actataagtt cgacgtcgaa gcgccgtggg gcagcctgag cgcgaacgtg
    37081 cataaagtgg tgttgtacgg ttctggcaaa gaaaatattg aattcaaata catgaacgat
    37141 cgtggcgata cctccattcg tcgtcatccg ttcgaaggcg tgctgcacaa tatggagcgc
    37201 cgttataaag agacggaatc cagtgcggta cgtgaagaat tagccaagtt tatcagcaat
    37261 cgtccgtgcg ccagctgcga aggaacccgt ctgcgtcggg aagcacgcca cgtgtatgtc
    37321 gagaatacgc cgctgcccac catctccgac atgagcatcg gtcatgcgat ggaattcttc
    37381 aacaatctca aactcgcagg tcagcgggcg aagattgcag aaaaaatcct taaagagatc
    37441 ggcgatcgtc tgaaattcct cgttaacgtc ggcctgaatt acctgacact ttcccgctca
    37501 gcagagacac tttccggcgg tgaagcccag cgtattcgtc tggcgagcca gattggtgca
    37561 ggcctggttg gcgtgatgta cgtgctggat gagccgtcta tcggcctgca ccagcgcgat
    37621 aacgagcgcc tgctgggtac gcttatccat ctgcgcgatc tcggtaatac cgtgattgtg
    37681 gtggagcacg acgaagacgc gattcgcgcc gctgatcatg tgatcgatat cggtccgggt
    37741 gcgggtgttc acggcggtga agtggtcgcg gaaggtccac tggaagcaat tatggcggtg
    37801 cctgagtcgt tgaccgggca gtacatgagc ggtaaacgca agattgaggt gccgaagaag
    37861 cgcgttccgg caaatccgga aaaagtgctg aaactgacag gcgcacgcgg caacaacctg
    37921 aaagacgtga cgctgacgct gccggtgggt ctgtttactt gcatcacagg ggtttcaggt
    37981 tccggtaaat cgacgctgat taacgacacg ctgtttccga ttgcccaacg ccagttgaat
    38041 ggcgcaacca tcgcggaacc tgcgccatat cgcgatattc agggactgga gcatttcgac
    38101 aaagtgatcg atatcgacca aagcccaatt ggtcgtaccc cacgttccaa tccggcgacc
    38161 tataccggcg tgtttacacc tgtacgcgaa ctgttcgcag gcgtaccaga atcccgtgcg
    38221 cgtggttata cgccagggcg tttcagtttt aacgtccgtg gcggacgttg cgaagcctgt
    38281 cagggcgacg gcgtgatcaa agtggagatg cacttcctgc cggacattta cgtaccgtgc
    38341 gaccagtgca aaggcaaacg ctataaccgt gaaacgctgg agattaagta caaaggcaaa
    38401 accatccacg aagtgctgga tatgaccatc gaagaggcgc gtgagttctt tgatgcggtg
    38461 ccagctctgg cgcgtaagct gcaaacgctg atggatgttg gcctgacgta cattcgcctc
    38521 gggcagtccg caaccacact ttctggtggt gaagcccagc gcgtgaagct ggcgcgtgag
    38581 ctgtcaaaac gcggcaccgg gcagacgctg tatattctcg acgagccgac caccggtctg
    38641 cacttcgccg atattcagca actgctcgac gtactgcata aactgcgcga tcagggcaat
    38701 accattgtgg taattgagca caatctcgac gtgatcaaaa ccgctgactg gattgtcgac
    38761 ctgggaccgg aaggcggcag tgggggcggc gaaatcctcg tctccggtac gccagaaacc
    38821 gtcgcagagt gcgaagcttc gcatacggcg cgcttcctca agccgatgct gtaatcgtta
    38881 aggccgcttt cagagcggcc ttcttctttc agagttgcac cagcaattta cgtttttctt
    38941 ccggcagcaa attcaccgcc tgctgataag acgcatccac cagataatag atttgcgaat
    39001 ccggcagcga accgtcgaga tacacggtgc tccagtgcgc tttattcaga tggcggcttg
    39061 gacgcacatc gctgtgctgc tgacgtagca gctccgccag ctccgggctg gttttcagcg
    39121 aaacagctgg gcgattttct acttctttca ccatcgcaaa cagcacatct tccactttga
    39181 tctgcgtcgc tttccagtcg ttatgcacgc tctgttctgc gcctggtttt gccatgcaat
    39241 attgtagcaa ctccgaaatg gtcatttttc actccccttg tagtgtcgcg atgatgcgac
    39301 gcgatccgcc gtggatgcga tgttccccca gccagatgcc ttgccaggtg ccggtctgaa
    39361 tacgcccttt atgcaccggc aatacaagcg atgttcccag cattgaggat ttgatatgag
    39421 aaggcatatc gtctgctccc tcatagtcat gctcataatt tccgttatcg ggaacggcgc
    39481 ggaggaaaaa acgctccatg tcgtggcgta cggtgggatc gcagttctca ttaagtgtca
    39541 gagaggcgga ggtatgttgc agcaacagat gcagtaagcc gatgttaacg cgcggcatat
    39601 cagccagctg attcagaatt tcatccgtta ccagatgaaa cccacgagat ttggcgctaa
    39661 gcgtgagcgt cttttgatac cacatgtgct gctccttgat aaaactctct taatcagttt
    39721 gcagcaagac agcgaaagga taaaggtgtg attaaaaaaa cagcattcgg gagagtatta
    39781 cgctctcccg ctctgtcagt attctgaatt gacgatcacc tcttcaccaa acgcacccgc
    39841 ttgtggcaag ggtttgtagg tagagttgga ggcgcgcaga atgcggatac cacgagcgcc
    39901 gacatcgcgt gcggcggtaa tatcgttatc ggaatcacca taaaaaattc ggatattttt
    39961 atcctgcagc cattgcgatt ttgtattttg ccctggttta tcgcccgcaa agatcaccgg
    40021 attcatgttg gtggcaggaa tatgaaaatt atccgccagc gtttttgaaa ccgtttctgt
    40081 tttggtcggg ctacgaccag tcacaaagaa gatcgcgtca ccgcggcgta catgcatatc
    40141 aatcagctgg cgagcgacct cttttggaat gctgaattca tcccagccat tgttcatttt
    40201 ttcccagaac acaggattct tcagataatc ttcgctttct ggcgagaagg tttttttgcc
    40261 gcgccagaaa cccggactgg aaaaaagtac cgtatcatcg atatcaaacc ccaccgccat
    40321 tggcggacgt cctgcgaggc tattttcaat ttgtgcaacc gaaacccaat gaattggcgc
    40381 ctgttcagcg agtttggcta cgttagtacc cggattaagc ggtgaaggag atgaggccag
    40441 ggcaacagcg gaactgttta gagcgaacaa taagcaaacg gcactcaatg cctgtgtgat
    40501 cttgcgcata tttttcccta aatagtcagt aagttgaaac ttattattgt gcgtttgttg
    40561 aaaaaaccat ctgaccataa cgccaaccgc gggggaaagg aaggattatc tgcggatttg
    40621 tcacataaaa agagcatgat cacatttgga atacctgtgc gataaaccac acattgccac
    40681 ggttattctg cttacacatt cacacaatgg cactcttttg gttctcgcaa acattgttca
    40741 gggaatatac tgatgataaa tataaatgca ttcaatggat gatgaatgaa aatatcgtgg
    40801 aattatatat ttaagaacaa atggcgattt cacattacaa gcatttcact ttttcttatc
    40861 atgctcgcgg tttcaatcgc ttttttgcac ttgcgtttta ataccttgtc cggtaccgat
    40921 aaaatgcggc ttgaaatgta taagtccaca ttatattcca ccatcgagca attttatgtt
    40981 ttaccctata tgctctcaac agaccatatc atccgtcagg cggtaattac gcctgacgat
    41041 atgacgtcca gcgaactcaa tcaacgaatt gcacatttca atactcaact caaaaccgca
    41101 gcaatattta ttctggatac ccaaggtaag gccatcgctt ctagcaactg gcaggacccc
    41161 ggcagctatg tagggcaaaa ttatagctat cgcccctatt ataaacacgc catgtctggc
    41221 ttaaatggac gcttttacgg tattggtagc actacaaata caccgggatt cttcctctct
    41281 acaagtataa aagataaagg aaaaattgtc ggtgttgtag tagtaaaaat aagtcttaat
    41341 gaaattgaaa aagcatgggc cgaaggtcct gaaaatatta tcgtgaatga tgaacatggg
    41401 attatatttt taagttcaaa atcgccatgg cgaatgcgaa cactgcaacc gttacctgtt
    41461 caggcaaaac aaaaactaca atctacccgt caatatagtc tcgacaatct tttaccggcg
    41521 gattattatc cctgttatac cgtgagcaat tttactttcc tgaaagataa aaaagaacaa
    41581 ctctgtttat tcccgcaata ttatacacaa caaatagcca ttccagaatt taactggaaa
    41641 atgacaatta tggtcccctt agataacctg tactggtcat gggctatttc gttagtcatt
    41701 acactaatta tttacctgct gtttttgtta tttattaaat actggagaat gcgatctcat
    41761 gcacaacaat tattaacact ggcgaatgaa acattagaaa aacaggttaa agagcgtaca
    41821 tctgccctgg aattgatcaa tcaaaaatta atacaggaga taaaagagcg cagtcaggct
    41881 gaacaagtat tacaaattac gcgtagtgaa ctggcagagt ccagcaaact ggcggcgctt
    41941 ggacagatgg caaccgaaat tgcccatgaa caaaatcaac cgttagccgc cattcacgca
    42001 cttactgata acgcgcgtac tatgctaaaa aaagagatgt atccgcaggt tgaacagaat
    42061 ctgaaacaca ttatttcagt gattgagcgg atgacgcagc tcatttccga acttaaagca
    42121 tttgcctcgc gccatcgcgt acctaaaggt tctgccgatg tcatcaaagt gatgtatagc
    42181 gccgtggcgt tacttaatca cagcatggag aaaaataaca ttgagcgacg aataaaagcc
    42241 ccatccatgc cgttatttgt caattgcgat gagctcggtc ttgaacagat attcagtaat
    42301 ttaattagca acgccttaga ttctatggaa ggtagctctt acaaacgact ggatatcgcc
    42361 attcgccagg caaataacaa agttattatt accattaaag acagcggtgg cggttttgca
    42421 cctgaagttg tcgatcgcat atttgaacca ttttttacca ctaaacgtag aggaatgggg
    42481 ttgggactgg caatagtcag cgaaattgtc cgaaattcga acggcgcact ccacgccagt
    42541 aatcatcctg aaggcggcgc agtaatgaca ttaacctggc ctgaatgggg agaagaacat
    42601 gaataataaa tccagtgaaa ttactatagt ttatattgag gatagtgacg atgtacgttt
    42661 tgcctgtgaa caaacgctca cgctggctgg ttatcgtgtt atttcctgtt gcgatgcaga
    42721 acacagtatt ccccttattc aaagtcaggc caacattatt atactgaccg atgtcaggtt
    42781 acctggaata tcaggactag agctcctcag ttacatcaac gaaatggata gcaaaattcc
    42841 ggttatttta attaccggac acggtgacgt ggaaatggcg gtggatgcca tgcgtaatgg
    42901 tgcattcgat ttcattgaga aaccaagtag ctcagataaa ttattgagta ttattgccag
    42961 agcggtagaa aaacggcgat tggttttaga aaatcagcaa ttactggcga atctgcaaca
    43021 ggaaaatggc ccggtgctga ttggtcgtag tccgcagatg caacaactgc gtaaaatgat
    43081 ccttaatgtc gcagataccg gtgccgatgt ccttatttat ggtgaaacag gttgtggtaa
    43141 agaagtcgtc gccagaatgc tgcatcactg gagtacccgc cgtcaggggc agtttgttgc
    43201 cttaaactgt gcaggcttac cagaaacgtt gtttgaaagc gaaatatttg gtcatgaggc
    43261 gggcgcattt accggcgcgg ttaaaaagcg tattggaaaa attgaacatg caaatggtgg
    43321 tacgttattt ctcgatgaaa tagagggaat gccatcagga atgcaggtaa aactgctacg
    43381 tgtattgcag gaaagaacca ttgaacgatt aggtgccaat caattaattc cggtgaattg
    43441 ccgtgttatt gcagcgacca aggaagattt actgcgtcgc agcgaagaac atctgtttcg
    43501 tctcgatctc tattatcgtc tgaatgtggt tagcctgaat attccacctc tacgccagcg
    43561 acgagaagat atccctgaac tgttttactg gttcgccagt caggcggcac aaaaatataa
    43621 tcgccctctg cctgatattt ctcccatgtt actggcctgg ttgcaatcac aaagttggcc
    43681 tggcaatgta cgtgaactta aacacaatgc agagcgtttt gttctcggtt tactgaccca
    43741 ccaccagcca gtgccaatga ctcagcaaga agagagtggc cttaccgcct gcattgatgc
    43801 tttcgagaaa aagttaatcg aagatatgct gcgacaaacg gaaggccagg tcagtttgac
    43861 agcgcggcta ttacagctgc cgcgcaagac tttatatgac aaactcaaca aacatcagat
    43921 tcaaccgcaa gtttatcgac cagaatcatc cagctaatgc tgattcctga ggatgtaacc
    43981 ccaaatcgat acctttgcgg gcgtaatgtt caactaccca ggaccagaat gataccggga
    44041 tcgtaatcat cttcgcattg cgttcccggt tggcagcttc tggcataccg gggtaaatca
    44101 ccggattgcc gccaggttga ggctgcgtag ctaacagata atccgcaaag aactgccctt
    44161 tctcccctgc cagatcggtg aattgatcag gatcgataat caacgcaaaa agatgattat
    44221 gcgcagatcc atccgtctga tgatcttcgg cgatcgtgtc accaccagat aagatccccg
    44281 ccatcatttc aatcatcagt gccaggccaa atcctttatg ctcaccaaat ggcagtaacg
    44341 cccctggagg attgctgaat aacaccccag gatcattgct tggattcccc tgtgcatcaa
    44401 tcaattgtcc cggcgcggcc tgcttcccgg tggacgacat tacccgtgct ttatttagcg
    44461 ccatgctact ggtggcaaaa tcaaccagaa tcggcggacg cccatcactg aacggccagg
    44521 caaagcagaa agggttagtg gtcatacgcg gttccgcacc gccccacggg gcgaccagtg
    44581 caggacgccc ggtgacgtta ctcatggcca gcatcgccag gccttgatca gcaaccattt
    44641 gcccgtaatg ccccatacgc cctaaatgat gcgcatggca caacgttaac aggcaaaaac
    44701 cgcggtgttt tgcttgttcg atggcagcat ttaccgccac ttttccggcg tgttgaccaa
    44761 agccattgtg ggcatgaaaa cgttgtaaat taccttcact tgttaagcat tccgggcggg
    44821 cattcgcggt tacatcgcca cgagcgatat tttccagata tttcggtagc agcgtgacgc
    44881 cgtggctggc aaagccataa cgttccgctt caatcatatg taccgccaca tcctgagcga
    44941 tatcggcatc acagccatta agttccagca gcccggcagc taaccaacgc actttttcaa
    45001 cagcaatttg taatgacatc gttggtttcc tcacattaga cctaaaattt tccaccaggt
    45061 ggcggcaaac agtagcaaca acaggtagcc gatcaccgtc aggtacagtc ccacacgggt
    45121 aaactggcgt ggcgtgaagg tatccgtacc catacacacc atattttgtg gcgcgttaat
    45181 cggcaggatg aagccaaaac tgacactgaa ggcgagcaga atggtcatgc cgaccgggtt
    45241 aacgccgagt tctggcggca gactgcttaa caggctaatc aggatgggca gcaatgcggc
    45301 agttagcgca gtggcactgg caaaccccag gtgaatgatt atcaagaaag cggcgagaat
    45361 cgcgaagatg gctaatgatg gcaagccatc gagaccaaaa cctttcacca catagttcgc
    45421 catccatgaa gctgcctgcg tatcaagcag cgtggacccc aggctgatac caataccaaa
    45481 catcagcaac gtgccccact gcacacgttt ttcgacctct ttccagttca ttacaccgat
    45541 gcctggcagc aacataatcg ccagtccggc aagggtgaca gaggtggtat caatactgtg
    45601 taattttccg ccagtagacc aaaagagcag taacaatagc gaaataccga tcaagcgttt
    45661 ttctttaccg gtggttggcc ctaactctgc gagtgctttt ttaatggcct cgctcccccc
    45721 ttcaacagct tccgtttctg gcggcaacaa tttacgtgcg aggaaataga ggatcaccga
    45781 catggtgagg ctccagggcg cgcccgctaa cagccagtca agccagctga cagaatgccc
    45841 ggcgccgaaa gttttattga taaaaccgat agaaagcagg ttttgcgccg ccgaagtttg
    45901 aataccaacg ttccagatac tggtcgcctg ggcgataaca atcatcattg acgccgctaa
    45961 acgtgaatgt ttatccacct taaatgccgc aatgaccccc atcataatcg gcaccacgca
    46021 agccgtacgg gcagtggcac tgggtacgac caggctcagg acgatggtca ccacaatggc
    46081 accgataata atgctgcggc tgctggcacc tattttcgac atagtaaaca gcgcaatgcg
    46141 tttatccagc ccggtaatgg tcattgccgc cgcgataaac attgctgctg ccaccagcgc
    46201 cagagcagaa ttagaaaaac ccgacagagt cattttcagc gctttcgcgg tgccaaggat
    46261 cgtgtccggg tgattcatat ccggggcaaa accaaccata aagataatca gtgcggaaat
    46321 aacaattgca ctggcggtgt aatccatcgc ttcgctgatc cagacaataa tggcaaacac
    46381 cagaatggcg atcatatttt gtccagcgac gggcaaggta tcgcccagag gcagaagagt
    46441 aataataatt ccggcaatga tcgccaacca caggccggga ttagggataa ttcgacgctt
    46501 ttgtggtgcg ccatgtgata ttgatgacat attcacctca ttgtgaaaaa taaaaaacct
    46561 gcctggctat tgcaggagc
//
LOCUS       DADSYM010000013        46389 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2415
            SAMN08039579-rid11723173.denovo.013, whole genome shotgun sequence.
ACCESSION   DADSYM010000013 DADSYM010000000
VERSION     DADSYM010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039579
            Sequence Read Archive: SRR6362533
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 46389)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 46389)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980162.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 03:39:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,484
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 4,347
            CDSs (with protein)               :: 4,347
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 43 of 85
            Pseudo Genes (incomplete)         :: 37 of 85
            Pseudo Genes (internal stop)      :: 19 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..46389
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039579-rid11723173.denovo.013"
                     /strain="LGE2415"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8302 N 95.9628 W"
                     /collection_date="2015-08-24"
     gene            complement(84..1388)
                     /locus_tag="J1Y83_000624"
     CDS             complement(84..1388)
                     /locus_tag="J1Y83_000624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HBA7628809.1"
                     /translation="MTTPLKKIVIVGGGAGGLEMATQLGHKLGRKKKAKITLVDRNHS
                     HLWKPLLHEVATGSLDEGVDALSYLAHARNHGFQFQLGSVIDIDREAKTITIAELRDE
                     KGELLVPERKIAYDTLVMALGSTSNDFNTPGVKENCIFLDNPHQARRFHQEMLNLFLK
                     YSANLGANGKVNIAIVGGGATGVELSAELHNAVKQLHSYGYKGLTNEALNVTLVEAGE
                     RILPALPPRISAAAHSELTKLGVRVLTQTMVTSADEGGLHTKDGEYIEADLMVWAAGI
                     KAPDFLKDIGGLETNRINQLVVEPTLQTTRDPDIYAIGDCASCPRPEGGFVPPRAQAA
                     HQMATCAMNNILAQMNGKPLKNYQYKDHGSLVSLSNFSTVGSLMGNLTRGSMMIEGRI
                     ARFVYISLYRMHQIALHGYFKTGLMMLVGSINRVIRPRLKLH"
     gene            complement(1787..2329)
                     /locus_tag="J1Y83_000625"
     CDS             complement(1787..2329)
                     /locus_tag="J1Y83_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBA7628810.1"
                     /translation="MIIYLHGFDSNSPGNHEKVLQLQFIDPDVRLISYSTRHPKHDMQ
                     HLLKEVDKMLQLNVDERPLICGVGLGGYWAERIGFLCDIRQVIFNPNLFPYENMEGKI
                     DRPEEYADIATKCVTNFREKNRDRCLVILSRNDEALNSQRTSEELHHYYEIVWDEEQT
                     HKFKNISPHLQRIKAFKTLG"
     gene            complement(2352..3377)
                     /gene="nagZ"
                     /locus_tag="J1Y83_000626"
     CDS             complement(2352..3377)
                     /gene="nagZ"
                     /locus_tag="J1Y83_000626"
                     /EC_number="3.2.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HBA7628811.1"
                     /translation="MGPVMLDVEGYELDAEEREILAHPLVGGLILFTRNYHDPAQLRE
                     LVRQIRAASRNHLVVAVDQEGGRVQRFREGFTRLPAAQSFAALLGMEEGGKLAQEAGW
                     LMASEMIAMDIDISFAPVLDVGHISAAIGERSYHADPQKALAIASRFIDGMHEAGMKT
                     TGKHFPGHGAVTADSHKETPCDPRPQAEIRAKDMSVFSSLIRENKLDAIMPAHVIYSD
                     VDPRPASGSPYWLKTVLRQELGFDGVIFSDDLSMEGAAIMGSYAERGQASLDAGCDMI
                     LVCNNRKGAVSVLDNLSPIKAERVTRLYHKGSFSRQELMDSARWKAISARLNQLHERW
                     QEEKAGH"
     gene            complement(3388..4212)
                     /gene="thiK"
                     /locus_tag="J1Y83_000627"
     CDS             complement(3388..4212)
                     /gene="thiK"
                     /locus_tag="J1Y83_000627"
                     /EC_number="2.7.1.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine kinase"
                     /protein_id="HBA7628812.1"
                     /translation="MPFRSNNPLTRDELLSRFFPQFHPVTTFNRGLSGGSFLIEHQGQ
                     RFVVRQPHDPDAPQSAFLRQYRALSQLPACIAPKPHLYLRDWMVVDYLPGEVKTYLPD
                     TNELAGLLYYLHQQPRFGWRITLLPLLELYWQQSDPARRTVGWLRMLKRLRKAREPRL
                     LRLSPLHMDVHAGNLVHSASGLKLIDWEYAGDGDIALELAAVWVENTDQHRQLVNDYA
                     TRAKIYPAQLWRQVRRWFPWLLMLKAGWFEYRWRQTGDQQFIRLADDTWRQLLIKQ"
     gene            complement(4193..4834)
                     /gene="lpoB"
                     /locus_tag="J1Y83_000628"
     CDS             complement(4193..4834)
                     /gene="lpoB"
                     /locus_tag="J1Y83_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator LpoB"
                     /protein_id="HBA7628813.1"
                     /translation="MTKMSRYALITALAMFLAGCVGQREPAPVEEVKPAPEQPAEPQQ
                     PVPTVPSVPTIPQQPGPIEHEDQTAPPAPHIRHYDWNGAMQPMVSKMLGADGVTAGSV
                     LLVDSVNNRTNGSLNAAEATETLRNALANNGKFTLVSAQQLSMAKQQLGLSPQDSLGT
                     RSKAIGIARNVGAHYVLYSSASGNVNAPTLQMQLMLVQTGEIIWSGKGAVSQQ"
     gene            complement(4848..5225)
                     /locus_tag="J1Y83_000629"
     CDS             complement(4848..5225)
                     /locus_tag="J1Y83_000629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcfL family protein"
                     /protein_id="HBA7628814.1"
                     /translation="MRKGCFGLMSLALLVLVGCRSHPEIPVNDEQSLVMESSLLAAGI
                     SAEKPVLSTSDIQPSASSTLYNERQEPVTVHYRFYWYDARGLEMHPLERPRSVTIPAH
                     SAVTLYGSANFLGAHKVRLYLYL"
     gene            complement(5228..5587)
                     /gene="hinT"
                     /locus_tag="J1Y83_000630"
     CDS             complement(5228..5587)
                     /gene="hinT"
                     /locus_tag="J1Y83_000630"
                     /EC_number="3.9.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415621.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine nucleoside phosphoramidase"
                     /protein_id="HBA7628815.1"
                     /translation="MAEETIFSKIIRREIPSDIVYQDDLVTAFRDISPQAPTHILIIP
                     NILIPTVNDVSAEHEQALGRMITVAAKIAEQEGIAEDGYRLIMNTNRHGGQEVYHIHM
                     HLLGGRPLGPMLAHKGL"
     gene            5922..8111
                     /gene="fhuE"
                     /locus_tag="J1Y83_000631"
     CDS             5922..8111
                     /gene="fhuE"
                     /locus_tag="J1Y83_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric-rhodotorulic acid/ferric-coprogen
                     receptor FhuE"
                     /protein_id="HBA7628816.1"
                     /translation="MLSTQFNRDNQHQAIIKPSLLAGCIALALLPSAAFAAPATEETV
                     IVEGSATAPDDGENDYNVTSTSAGTKMQMTQRDIPQSVTIVSQQRMEDQQLQTLGEVM
                     ENTLGISKSQADSDRALYYSRGFQIDNYMVDGIPTYFESRWNLGDALSDMALFERVEV
                     VRGATGLMTGTGNPSAAINMVRKHATSREFKGDVSAEYGSWNKERYVADLQSPLTEDG
                     KIRARIVGGYQNNDSWLDRYNSEKTFFSGIVDADLGDLTMLSAGYEYQRIDVNSPTWG
                     GLPRWNTDGSSNSYDRARSTAPDWAYNDKEINKVFMTLKQRFADTWQATLNATHSEVE
                     FDSKMMYVDAYVNKADGMLVGPYSNYGPGFDYVGGTGWNSGKRKVDALDLFADGSYEL
                     FGRQHNLMFGGSYSKQNNRYFSSWANIFPDEIGSFYNFNGNFPQTDWSPQSLAQDDTT
                     HMKSLYAATRVTLADPLHLILGARYTNWRVDTLTYSMEKNHTTPYAGLVFDINDNWST
                     YASYTSIFQPQNDRDSSGKYLTPITGNNYELGLKSDWMNSRLTTTLAIFRIEQDNVAQ
                     STGTPIPGSNGETAYKAVDGTVSKGVEFELNGAITDNWQLTFGATRYIAEDNEGNAVN
                     PNLPRTTVKMFTSYRLPVMPELTVGGGVNWQNRVYTDTVTPYGTFRAEQGSYALVDLF
                     TRYQVTKNFSLQGNVNNLFDKTYDTNVEGSIVYGAPRNFSITGTYQF"
     gene            complement(8171..9604)
                     /gene="ptsG"
                     /locus_tag="J1Y83_000632"
     CDS             complement(8171..9604)
                     /gene="ptsG"
                     /locus_tag="J1Y83_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000475716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIBC"
                     /protein_id="HBA7628817.1"
                     /translation="MFKNAFANLQKVGKSLMLPVSVLPIAGILLGVGSANFSWLPAVV
                     SHVMAEAGGSVFANMPLIFAIGVALGFTNNDGVSALAAVVAYGIMVKTMAVVAPLVLH
                     LPAEEIASKHLADTGVLGGIISGAIAAYMFNRFYRIKLPEYLGFFAGKRFVPIISGLA
                     AIFTGVVLSFIWPPIGSAIQTFSQWAAYQNPVVAFGIYGFIERCLVPFGLHHIWNVPF
                     QMQIGEYTNAAGQVFHGDIPRYMAGDPTAGKLSGGFLFKMYGLPAAAIAIWHSAKPEN
                     RAKVGGIMISAALTSFLTGITEPIEFSFMFVAPILYIIHAILAGLAFPICILLGMRDG
                     TSFSHGLIDFIVLSGNSSKLWLFPIVGIGYAIVYYTIFRVLIKALDLKTPGREDATED
                     AKATGTSEMAPALVAAFGGKENITNLDACITRLRVSVADVSKVDQAGLKKLGAAGVVV
                     AGSGVQAIFGTKSDNLKTEMDEYIRNH"
     gene            complement(9899..10696)
                     /locus_tag="J1Y83_000633"
     CDS             complement(9899..10696)
                     /locus_tag="J1Y83_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HBA7628818.1"
                     /translation="MFLVDSHCHLDGLDYESLHKDVDDVLAKAAARDVKFCLAVATTL
                     PGYLHMRDLVGERDNVVFSCGVHPLNQNDPYDVEDLRRLAAEEGVVALGETGLDYYYT
                     PETKVRQQESFIHHIQIGRELNKPVIVHTRDARADTLAILREEKVTDCGGVLHCFTED
                     RETAGKLLDLGFYISFSGIVTFRNAEQLRDAARYVPLDRLLVETDSPYLAPVPHRGKE
                     NQPAMVRDVAEYMAVLKGVAVEELAQVTTDNFARLFHIDASRLQSIR"
     gene            complement(10707..11711)
                     /gene="holB"
                     /locus_tag="J1Y83_000634"
     CDS             complement(10707..11711)
                     /gene="holB"
                     /locus_tag="J1Y83_000634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HBA7628819.1"
                     /translation="MRWYPWLRPDFEKLVASYQAGRGHHALLIQALPGMGDDALIYAL
                     SRYLLCQQPQGHKSCGHCRGCQLMQAGTHPDYYTLAPEKGKNALGIDAVREVTEKLNE
                     HARLGGAKVVWVTDAALLTDAAANALLKTLEEPPAETWFFLATREPERLLATLRSRCR
                     LHYLAPPPEQYAVTWLSREVTMSQDALLAALRLSAGSPGAALALFQGDNWQARETLCQ
                     ALAYSVQSGDWYSLLAALNHEQAPARLHWLATLLMDALKRHYGAAQVTNVDVPGLVVE
                     LANHLSPSRLQAILGDVCHIREQLMSVTGINRELLITDLLLRIEHYLQPGVVLPVPHL
                     "
     gene            complement(11708..12349)
                     /locus_tag="J1Y83_000635"
     CDS             complement(11708..12349)
                     /locus_tag="J1Y83_000635"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HBA7628820.1"
                     /translation="MRSKYIVIEGLEGAGKTTARNVVVETLEQLGIRDMVFTREPGGT
                     QLAEKLRSLVLDIKSVGDEVITDKAEVLMFYAARVQLVETVIKPALANGTWVIGDRHD
                     LSTQAYQGGGRGIDQHMLATLRDAVLGDFRPDLTLYLDVTPEVGLKRARARGELDRIE
                     QESFDFFNRTRARYLELAAQDKSIHTIDATQPLEAVMGAIRTTVTHWVKELDA"
     gene            complement(12339..13361)
                     /gene="yceG"
                     /locus_tag="J1Y83_000636"
     CDS             complement(12339..13361)
                     /gene="yceG"
                     /locus_tag="J1Y83_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein YceG"
                     /protein_id="HBA7628821.1"
                     /translation="MKKVLLIILLLLVVLGIAAGVGVWKVRHLADSKLLIKEETIFTL
                     KPGTGRLALGEQLYADKIINRPRVFQWLLRIEPDLSHFKAGTYRFTPQMTVREMLKLL
                     ESGKEAQFPLRLVEGMRLSDYLKQLREAPYIKHTLSDDKYATVAQALELENPEWIEGW
                     FWPDTWMYTANTTDVALLKRAHKKMVKAVDSAWEGRADGLPYKDKNQLVTMASIIEKE
                     TAVASERDQVASVFINRLRIGMRLQTDPTVIYGMGERYNGKLSRADLETPTAYNTYTI
                     TGLPPGAIATPGADSLKAAAHPAKTPYLYFVADGKGGHTFNTNLASHNKSVQDYLKVL
                     KEKNAQ"
     gene            complement(13364..14173)
                     /gene="pabC"
                     /locus_tag="J1Y83_000637"
     CDS             complement(13364..14173)
                     /gene="pabC"
                     /locus_tag="J1Y83_000637"
                     /EC_number="4.1.3.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate lyase"
                     /protein_id="HBA7628822.1"
                     /translation="MFLINGHKQESLAVSDRATQFGDGCFTTARVIDGKVSLLSAHIQ
                     RLQDACQRLMISCDFWPQLEQEMKTLAAEQQNGVLKVVISRGSGGRGYSTLNSGPATR
                     VLSVTAYPAHYDRLRNEGMTLALSPVRLGRNPHLAGIKHLNRLEQVLIRSHLEQTNAD
                     EALVLDSEGWVTECCAANLFWRKGNVVYTPRLDQAGVNGIMRQFCIRLLAQSSYQLVE
                     VQASLEEALQADEMVICNALMPVMPVRACGDVSFSSATLYEYLAPLCERPN"
     gene            complement(14293..15534)
                     /gene="fabF"
                     /locus_tag="J1Y83_000638"
     CDS             complement(14293..15534)
                     /gene="fabF"
                     /locus_tag="J1Y83_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HBA7628823.1"
                     /translation="MSKRRVVVTGLGMLSPVGNTVESTWKALLAGQSGISLIDHFDTS
                     AYATKFAGLVKDFNCEDIISRKEQRKMDAFIQYGIVAGVQAMQDSGLEITEENATRIG
                     AAIGSGIGGLGLIEENHTSLMNGGPRKISPFFVPSTIVNMVAGHLTIMYGLRGPSISI
                     ATACTSGVHNIGHAARIIAYGDADVMVAGGAEKASTPLGVGGFGAARALSTRNDNPQA
                     ASRPWDKERDGFVLGDGAGMLVLEEYEHAKKRGAKIYAELVGFGMSSDAYHMTSPPEN
                     GAGAALAMANALRDAGIEASQIGYVNAHGTSTPAGDKAEAQAVKTIFGEAASRVLVSS
                     TKSMTGHLLGAAGAVESIYSILALRDQAVPPTINLDNPDEGCDLDFVPHEARQVSGME
                     YTLCNSFGFGGTNGSLIFKKI"
     gene            complement(15622..15858)
                     /gene="acpP"
                     /locus_tag="J1Y83_000639"
     CDS             complement(15622..15858)
                     /gene="acpP"
                     /locus_tag="J1Y83_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016535861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HBA7628824.1"
                     /translation="MSTIEERVKKIIGEQLGVKQEEVTNNASFVEDLGADSLDTVELV
                     MALEEEFDTEIPDEEAEKITTVQAAIDYINGHQA"
     gene            complement(16069..16803)
                     /gene="fabG"
                     /locus_tag="J1Y83_000640"
     CDS             complement(16069..16803)
                     /gene="fabG"
                     /locus_tag="J1Y83_000640"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HBA7628825.1"
                     /translation="MNFEGKIALVTGASRGIGRAIAETLAARGAKVIGTATSENGAQA
                     ISDYLGANGKGLMLNVTDPASIESVLEKIRAEFGEVDILVNNAGITRDNLLMRMKDEE
                     WNDIIETNLSSVFRLSKAVMRAMMKKRHGRIITIGSVVGTMGNGGQANYAAAKAGLIG
                     FSKSLAREVASRGITVNVVAPGFIETDMTRALSDDQRAGILAQVPAGRLGGAQEIANA
                     VAFLASDEAAYITGETLHVNGGMYMV"
     gene            complement(16816..17745)
                     /gene="fabD"
                     /locus_tag="J1Y83_000641"
     CDS             complement(16816..17745)
                     /gene="fabD"
                     /locus_tag="J1Y83_000641"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HBA7628826.1"
                     /translation="MTQFAFVFPGQGSQTVGMLADMAASYPIVEETFAEASAALGYDL
                     WALTQQGPAEELNKTWQTQPALLTASVALYRVWQQQGGKAPAMMAGHSLGEYSALVCA
                     GVIDFADAVRLVEMRGKFMQEAVPEGTGAMAAIIGLDDASIGKACEEAAEGQVVSPVN
                     FNSPGQVVIAGHKEAVERAGAACKAAGAKRALPLPVSVPSHCALMKPAADKLAVELAK
                     ITFNAPTVPVVNNVDVKCETNGDAIRDALVRQLYNPVQWTKSVEYMAAQGVEHLYEVG
                     PGKVLTGLTKRIVDTLTASALNEPSAMAAALEL"
     gene            complement(17761..18714)
                     /locus_tag="J1Y83_000642"
     CDS             complement(17761..18714)
                     /locus_tag="J1Y83_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III"
                     /protein_id="HBA7628827.1"
                     /translation="MYTKIIGTGSYLPEQVRTNADLEKMVDTSDEWIVTRTGIRERHI
                     AAPNETVSTMGFEAATRAIEMAGIEKDQIGLIVVATTSATHAFPSAACQIQSMLGIKG
                     CPAFDVAAACAGFTYALSVADQYVKSGAVKYALVVGSDVLARTCDPTDRGTIIIFGDG
                     AGAAVLAASEEPGIISTHLHADGSYGELLTLPNADRVNPENSIHLTMAGNEVFKVAVT
                     ELAHIVDETLAANNLDRSQLDWLVPHQANLRIISATAKKLGMSMDNVVVTLDRHGNTS
                     AASVPCALDEAVRDGRIKPGQLVLLEAFGGGFTWGSALVRF"
     gene            complement(18782..19852)
                     /gene="plsX"
                     /locus_tag="J1Y83_000643"
     CDS             complement(18782..19852)
                     /gene="plsX"
                     /locus_tag="J1Y83_000643"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415608.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HBA7628828.1"
                     /translation="MTRLTLALDVMGGDFGPSVTVPAALQALNSNSQLTLLLVGNPDA
                     ITPLLAKADFEQRSRLQIIPAQSVIASDARPSQAIRASRGSSMRVALELVKEGRAQAC
                     VSAGNTGALMGLAKLLLKPLEGIERPALVTVLPHQQKGKTVVLDLGANVDCDSTMLVQ
                     FAIMGSVLAEEVVEIPNPRVALLNIGEEEVKGLDSIRDASAVLKTIPSINYIGYLEAN
                     ELLTGKTDVLVCDGFTGNVTLKTMEGVVRMFLSLLKSQGEGKKRSWWLLLLKRWLQKS
                     LTRRFSHLNPDQYNGACLLGLRGTVIKSHGAANQRAFAVAIEQAVQAVQRQVPQRIAA
                     RLESVYPAGFELLDGGKSGTLR"
     gene            complement(19933..20106)
                     /gene="rpmF"
                     /locus_tag="J1Y83_000644"
     CDS             complement(19933..20106)
                     /gene="rpmF"
                     /locus_tag="J1Y83_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HBA7628829.1"
                     /translation="MAVQQNKPTRSKRGMRRSHDALTAVTSLSVDKTSGEKHLRHHIT
                     ADGYYRGRKVIAK"
     gene            complement(20158..20679)
                     /gene="yceD"
                     /locus_tag="J1Y83_000645"
     CDS             complement(20158..20679)
                     /gene="yceD"
                     /locus_tag="J1Y83_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001174481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA accumulation protein YceD"
                     /protein_id="HBA7628830.1"
                     /translation="MQKVKLPLTLDPVRTAQKRLDYQGIYTPDQVERVAESVVSVDSD
                     VECSMSFAIDNQRLAVLNGDAKVTVTLECQRCGKPFTHQVYTTYCFSPVRSDEQAEAL
                     PEAYEPIEVNEFGEIDLLAMVEDEIILALPVVPVHDSEHCEVSEADMVFGELPEEAQK
                     PNPFAVLASLKRK"
     gene            20878..21462
                     /gene="yceF"
                     /locus_tag="J1Y83_000646"
     CDS             20878..21462
                     /gene="yceF"
                     /locus_tag="J1Y83_000646"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415605.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-methyl-GTP pyrophosphatase"
                     /protein_id="HBA7628831.1"
                     /translation="MPKLILASTSPWRRALLEKLQISFECAAPEVDETPRSDESPRQL
                     VLRLAQEKAQSLASRYPDHLIIGSDQVCVLDGEITGKPLTEENARLQLRKASGNIVTF
                     YTGLALFNSANGHLQTEVEPFDVHFRHLSEAEIDNYVRKEHPLHCAGSFKSEGFGITL
                     FERLEGRDPNTLVGLPLIALCQMLRREEKNPLMG"
     gene            complement(21574..22533)
                     /gene="rluC"
                     /locus_tag="J1Y83_000647"
     CDS             complement(21574..22533)
                     /gene="rluC"
                     /locus_tag="J1Y83_000647"
                     /EC_number="5.4.99.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(955/2504/2580) synthase
                     RluC"
                     /protein_id="HBA7628832.1"
                     /translation="MKTETPSVKIVAITADEAGQRIDNFLRTQLKGVPKSMIYRILRK
                     GEVRVNKKRIKPEYKLEAGDEVRIPPVRVAEREEEAVSPHLQKVAALADVILYEDDHI
                     LVLNKPSGTAVHGGSGLSFGVIEGLRALRPEARFLELVHRLDRDTSGVLLVAKKRSAL
                     RSLHEQLREKGMQKDYLALVRGQWQSHVKSVQAPLLKNILQSGERIVRVSQEGKPSET
                     RFKVEERYAFATLVRCSPVTGRTHQIRVHTQYAGHPIAFDDRYGDREFDRQLTEAGTG
                     LNRLFLHAAALKFTHPGTGEVMRIEAPMDEGLKRCLQKLRNAR"
     gene            23106..26291
                     /gene="rne"
                     /locus_tag="J1Y83_000648"
     CDS             23106..26291
                     /gene="rne"
                     /locus_tag="J1Y83_000648"
                     /EC_number="3.1.26.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E"
                     /protein_id="HBA7628833.1"
                     /translation="MKRMLINATQQEELRVALVDGQRLYDLDIESPGHEQKKANIYKG
                     KITRIEPSLEAAFVDYGAERHGFLPLKEIAREYFPANYSAHGRPNIKDVLREGQEVIV
                     QIDKEERGNKGAALTTFISLAGSYLVLMPNNPRAGGISRRIEGDDRTELKEALASLEL
                     PEGMGLIVRTAGVGKSAEALQWDLSFRLKHWEAIKKAAESRPAPFLIHQESNVIVRAF
                     RDYLRQDIGEILIDNPKVLELARQHIAALGRPDFSSKIKLYTGEIPLFSHYQIESQIE
                     SAFQREVRLPSGGSIVIDSTEALTAIDINSARATRGGDIEETAFNTNLEAADEIARQL
                     RLRDLGGLIVIDFIDMTPVRHQRAVENRLREAVRQDRARIQISHISRFGLLEMSRQRL
                     SPSLGESSHHVCPRCSGTGTVRDNESLSLSILRLIEEEALKENTQEVHAIVPVPIASY
                     LLNEKRSAVNAIETRQDGVRCVIVPNDQMETPHYHVLRVRKGEETSTLSYMLPKLHEE
                     AMALPSEEEFAERKRPEQPALATFAMPDVPPAPTPAEPAAPVVAPAPKAATATPASPA
                     QPGLLSRFFGALKALFSGGEETKPAEQSAPKAEAKPERQQDRRKPRQNNRRDRNERRD
                     TRSERTEGSDNREENRRNRRQAQQQTAETRESRQQAEVTEKARTTDEQQAPRRERSRR
                     RNDDKRQAQQEVKALNVEEQSVQETEQEERVRPVQPRRKQRQLNQKVRYEQSVAEEAV
                     VAPVVEETVAAEPIVQEAAAPRTELVKVPLPVVAQTAPEQQEENNADNRDNGGMPRRS
                     RRSPRHLRVSGQRRRRYRDERYPTQSPMPLTVACASPELASGKVWIRYPIVRPQDVQV
                     EEQREQEEVQVQPMVTEVPVAAAAEPVVSAPVVEEVAEVVEAPVQVAEPQPEVVETTH
                     PEVIAAAVTEQPQVITESDVAVAQEVAEHAEPVVEPQEETADIEEVAETAEVVVAEPE
                     VVAQPAAPVVAEVTAEVETVAAVEPEVTVEHNHATAPMTRAPAPEYVPEAPRHSDWQR
                     PTFTFEGKGAAGGHTATHHASAAPARPQPVE"
     gene            complement(26486..27439)
                     /gene="flgL"
                     /locus_tag="J1Y83_000649"
     CDS             complement(26486..27439)
                     /gene="flgL"
                     /locus_tag="J1Y83_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-filament junction protein FlgL"
                     /protein_id="HBA7628834.1"
                     /translation="MRFSTQMMYQQNMRGITNSQAEWMKYGEQMSTGKRVVNPSDDPI
                     AASQAVVLSQAQAQNSQYTLARTFATQKVSLEESVLSQVTTAIQNAQEKIVYASNGTL
                     SDDDRASLATDIQGLRDQLLNLANTTDGNGRYIFAGYKTETAPFSEADGDYVGGTESI
                     KQQVDASRSMVIGHTGDKIFDSITSNAVAEPDGSASETNLFAMLDSAIAALKTPVADS
                     EADKETAAAALDKTNRGLKNSLNNVLTVRAELGTQLNELESLDSLGSDRALGQTQQMS
                     DLVDVDWNATISSYIMQQTALQASYKAFTDMQGLSLFQLNK"
     gene            complement(27451..29094)
                     /gene="flgK"
                     /locus_tag="J1Y83_000650"
     CDS             complement(27451..29094)
                     /gene="flgK"
                     /locus_tag="J1Y83_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgK"
                     /protein_id="HBA7628835.1"
                     /translation="MSSLINNAMSGLNAAQVALNTASNNISSYNVAGYTRQTTIMAQA
                     NSTLGAGGWVGNGVYVSGVQREYDAFITNQLRAAQTQSSGLTARYEQMSKIDNMLSTS
                     TSSLATQMQDFFTSLQTLVSNAEDPAARQALIGKSEGLVNQFKTTDQYLRDQDKQVNI
                     AIGASVDQINNYAKQIASLNDQISRLTGVGAGASPNNLLDQRDQLVSELNQIVGVEVS
                     VQDGGTYNITMVNGYSLVQGSTARQLAAVPSSADPSRTTVAYIDGTAGNIEIPEKLLN
                     TGSLGGILTFRSQDLDQTRNTLGQLALAFAEAFNTQHKAGFDANGDAGKDFFAIGKPA
                     VLQNTKNNGDVAIGATVTDASAVLATDYKISFDNNQWQVTRLASNTTFTVTPDANGKV
                     AFDGLELTFTGTPAVNDSFTLKPVSDAIVNMDVLITDEAKIAMASEEDAGDSDNRNGQ
                     ALLDLQSNSKTVGGAKSFNDAYASLVSDIGNKTATLKTSSATQGNVVTQLSNQQQSIS
                     GVNLDEEYGNLQRFQQYYLANAQVLQTANAIFDALINIR"
     gene            complement(29160..30101)
                     /gene="flgJ"
                     /locus_tag="J1Y83_000651"
     CDS             complement(29160..30101)
                     /gene="flgJ"
                     /locus_tag="J1Y83_000651"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly peptidoglycan hydrolase FlgJ"
                     /protein_id="HBA7628836.1"
                     /translation="MISDSKLLASAAWDAQSLNELKAKAGEDPAANIRPVARQVEGMF
                     VQMMLKSMRDALPKDGLFSSEHTRLYTSMYDQQIAQQMTAGKGLGLAEMMVKQMTPEQ
                     PLPEEPTPAAPMKFPLETVVRYQNQTLSQLVQKAVPRNYDDSLPGDSKAFLAQLSLPA
                     QLASQQSGVPHHLILAQAALESGWGQRQIRRENGEPSYNLFGVKASGNWKGPVTEITT
                     TEYENGEAKKVKAKFRVYSSYLEALSDYVGLLTRNPRYAAVTTAASAEQGAQALQDAG
                     YATDPHYARKLTSMIQQMKSISDKVSKTYSMNIDNLF"
     gene            complement(30101..31198)
                     /locus_tag="J1Y83_000652"
     CDS             complement(30101..31198)
                     /locus_tag="J1Y83_000652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415598.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring protein FlgI"
                     /protein_id="HBA7628837.1"
                     /translation="MIKFLSALILLLVTTAAQAERIRDLTSVQGVRQNSLIGYGLVVG
                     LDGTGDQTTQTPFTTQTLNNMLSQLGITVPTGTNMQLKNVAAVMVTASLPPFGRQGQT
                     IDVVVSSMGNAKSLRGGTLLMTPLKGVDSQVYALAQGNILVGGAGASAGGSSVQVNQL
                     NGGRITNGAVIERELPSQFGVGNALNLQLNDEDFSMAQQIADTINRVRGYGSATALDA
                     RTIQVRVPSGNSSQVRFLADIQNMQVNVTPQDAKVVINSRTGSVVMNREVTLDSCAVA
                     QGNLSVTVNRQANVSQPDTPFGGGQTVVTPQTQIDLRQSGGSLQSVRSSASLNNVVRA
                     LNALGATPMDLMSILQSMQSAGCLRAKLEII"
     gene            complement(31210..31908)
                     /gene="flgH"
                     /locus_tag="J1Y83_000653"
     CDS             complement(31210..31908)
                     /gene="flgH"
                     /locus_tag="J1Y83_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body L-ring protein FlgH"
                     /protein_id="HBA7628838.1"
                     /translation="MQKNAAHTYAISSLLVLSLTGCAWIPSTPLVQGATSAQPVPGPT
                     PVANGSIFQSAQPINYGYQPLFEDRRPRNIGDTLTIVLQENVSASKSSSANASRDGKT
                     NFGFDTVPRYLQGLFGNARADVEASGGNTFNGKGGANASNTFSGTLTVTVDQVLVNGN
                     LHVVGEKQIAINQGTEFIRFSGVVNPRTISGSNTVPSTQVADARIEYVGNGYINEAQN
                     MGWLQRFFLNLSPM"
     gene            complement(31961..32743)
                     /gene="flgG"
                     /locus_tag="J1Y83_000654"
     CDS             complement(31961..32743)
                     /gene="flgG"
                     /locus_tag="J1Y83_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006819466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgG"
                     /protein_id="HBA7628839.1"
                     /translation="MISSLWIAKTGLDAQQTNMDVIANNLANVSTNGFKRQRAVFEDL
                     LYQTIRQPGAQSSEQTTLPSGLQIGTGVRPVATERLHSQGNLSQTNNSKDVAIKGQGF
                     FQVMLPDGSSAYTRDGSFQVDQNGQLVTAGGFQVQPAITIPANALSITIGRDGVVSVT
                     QQGQAAPVQVGQLNLTTFMNDTGLESIGENLYTETQSSGAPNESTPGLNGAGLLYQGY
                     VETSNVNVAEELVNMIQVQRAYEINSKAVSTTDQMLQKLTQL"
     gene            complement(32915..33670)
                     /locus_tag="J1Y83_000655"
     CDS             complement(32915..33670)
                     /locus_tag="J1Y83_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgF"
                     /protein_id="HBA7628840.1"
                     /translation="MDHAIYTAMGAASQTLNQQAVTASNLANASTPGFRAQLNALRAV
                     PVEGLSLPTRTLVTASTPGADMTPGKMDYTSRPLDVALQQDGWLVVQTADGSEGYTRN
                     GSIQVDPTGQLTIQGHPVVGEAGPIAVPEGAEITIAADGTISALNLGDPANTVAPVGR
                     LKLVKATGSEVQRGDDGIFRLSAESQARRGPVLQADPTLRVMSGVLEGSNVNAVAAMS
                     DMIASARRFEMQMKVISSVDDNAGRANQLLSMS"
     gene            complement(33690..35615)
                     /gene="flgD"
                     /locus_tag="J1Y83_000656"
                     /pseudo
     CDS             complement(33690..35615)
                     /gene="flgD"
                     /locus_tag="J1Y83_000656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706995.3"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook assembly protein FlgD"
     gene            complement(35627..36031)
                     /gene="flgC"
                     /locus_tag="J1Y83_000657"
     CDS             complement(35627..36031)
                     /gene="flgC"
                     /locus_tag="J1Y83_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgC"
                     /protein_id="HBA7628841.1"
                     /translation="MALLNIFDIAGSALTAQSQRLNVAASNLANADSVTGPDGQPYRA
                     KQVVFQVNAAPGAATGGVKVADVIESQAPDKLVYEPGNPLADAKGYVKMPNVDVVGEM
                     VNTMSASRSYQANVEVLNTVKSMMLKTLTLGQ"
     gene            complement(36035..36451)
                     /gene="flgB"
                     /locus_tag="J1Y83_000658"
     CDS             complement(36035..36451)
                     /gene="flgB"
                     /locus_tag="J1Y83_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgB"
                     /protein_id="HBA7628842.1"
                     /translation="MLDKLDAALRFQQEALNLRAQRQEVLAANIANADTPGYQARDID
                     FASELKKVMQRGRDATSVVALTMTSTKHIPAQALTPPTAELQYRIPDQPSLDGNTVDM
                     DRERTQFADNSLQYQMSLSALSGQIKGMMNVLQSGN"
     gene            36606..37265
                     /gene="flgA"
                     /locus_tag="J1Y83_000659"
     CDS             36606..37265
                     /gene="flgA"
                     /locus_tag="J1Y83_000659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring formation protein
                     FlgA"
                     /protein_id="HBA7628843.1"
                     /translation="MLTIKRSVAIIAILFSPLSTASNLTSQLHNFFSAQLAGVSDEVR
                     VSIRTAPNLLPPCDQPLLSMGNNSRLWGNVNVLARCGNDKRYLQVNVQATGNYVVAAM
                     PIVRGGKLEAGNVKLKRGRLDTLPPRTVLDINQLVDAVSLRDLSPDQPIQLTHFRQAW
                     RVKAGQRVNVIASGDGFSANAEGQALNNAAVAQNARVRMISGQVVSGVVDADGNILIN
                     L"
     gene            37341..37634
                     /gene="flgM"
                     /locus_tag="J1Y83_000660"
     CDS             37341..37634
                     /gene="flgM"
                     /locus_tag="J1Y83_000660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma-28 factor FlgM"
                     /protein_id="HBA7628844.1"
                     /translation="MSIDRTSPLKPVSTVQPRETTDAPVTNTRAAKTTASTSTSVTLS
                     DAQAKLMQPGSSDINLERVEALKLAIRNGELKMDTGKIADALINEAQQDLQSN"
     gene            37639..38055
                     /gene="flgN"
                     /locus_tag="J1Y83_000661"
     CDS             37639..38055
                     /gene="flgN"
                     /locus_tag="J1Y83_000661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis chaperone FlgN"
                     /protein_id="HBA7628845.1"
                     /translation="MTRLAEILDQMSAVLNDLKTVMDQEQQHLSMGQINGSQLQWITE
                     QKSSLLATLDYLEQLRRKEPNTANSVDISQRWQEITGKTQQLRQLNQHNGWLLEGQIE
                     RNQQALEMLKPHQEPTLYGANGQTSTTHRGSKKISI"
     gene            complement(38095..39630)
                     /gene="murJ"
                     /locus_tag="J1Y83_000662"
     CDS             complement(38095..39630)
                     /gene="murJ"
                     /locus_tag="J1Y83_000662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706990.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HBA7628846.1"
                     /translation="MNLLKSLAAVSSMTMFSRVLGFARDAIVARIFGAGMATDAFFVA
                     FKLPNLLRRIFAEGAFSQAFVPILAEYKSKQGEDATRVFVSYVSGLLTLALAVVTVAG
                     MLAAPWVIMVTAPGFADTADKFALTSQLLKITFPYILLISLASLVGAILNTWNRFSIP
                     AFAPTLLNISMIGFALFAAPYFNPPVLALAWAVTVGGILQLVYQLPHLKKIGMLVLPR
                     INFHDAGAMRVVKQMGPAILGVSVSQISLIINTIFASFLASGSVSWMYYADRLMEFPS
                     GVLGVALGTILLPSLSKSFASGNHDEYNRLMDWGLRLCFLLALPSAVALGILSGPLTV
                     SLFQYGKFTAFDALMTQRALIAYSVGLIGLIVVKVLAPGFYSRQDIKTPVKIAIVTLI
                     LTQLMNLAFIGPLKHAGLSLSIGLAACLNASLLYWQLRKQKIFTPQPGWMAFLLRLVV
                     AVLVMSGVLLGMLHIMPEWSLGTMPWRLLRLMAVVLAGIAAYFAALAVLGFKVKEFAR
                     RTV"
     gene            complement(39740..40663)
                     /locus_tag="J1Y83_000663"
     CDS             complement(39740..40663)
                     /locus_tag="J1Y83_000663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HBA7628847.1"
                     /translation="MKKLRIGVVGLGGIAQKAWLPVLAAASDWTLQGAWSPTRAKALL
                     ICDSWRIPYADSLSSLAASCDAVFVHSSTASHFDVVSTLLNAGVHVCVDKPLAENLRD
                     AERLVELAARKKLTLMVGFNRRFAPLYGELKTQLATASSLRMDKHRTNSVGPHDLYFT
                     LLDDYLHVVDTALWLSGGKASLDGGTLLTNDAGEMLFAEHHFSAGPLQITTCMHRRAG
                     SQRETVQAVTDGALIDITDMREWREERGQGVVHKPIPSWQSTLEQRGFVGCARHFIEC
                     VQNQTVPQTAGEQAVLAQRIVDKIWRDAMSE"
     gene            complement(40665..41312)
                     /locus_tag="J1Y83_000664"
     CDS             complement(40665..41312)
                     /locus_tag="J1Y83_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF480 domain-containing protein"
                     /protein_id="HBA7628848.1"
                     /translation="MKYQLTVMEARVIGCLLEKQVTTPEQYPLSVNGVVTACNQKTNR
                     EPVMNLSESEVQEQLDNLVKRHYLRTVSGFGNRVTKYEQRFCNSEFGDLKLSAAEVAL
                     ITTLLLRGAQTPGELRSRAARMYEFSDMAEVESTLEQLANREDGPFVVRLAREPGKRE
                     SRYMHLFSGEVENPPAVTDMSNAADGDLQARVEALEIEVAELKQRLDSLLAHLGD"
     gene            complement(41323..41907)
                     /gene="rimJ"
                     /locus_tag="J1Y83_000665"
     CDS             complement(41323..41907)
                     /gene="rimJ"
                     /locus_tag="J1Y83_000665"
                     /EC_number="2.3.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S5-alanine
                     N-acetyltransferase"
                     /protein_id="HBA7628849.1"
                     /translation="MFGYRSNVPKVRLTTDRLVVRLVHDRDAWRLADYYAENRHFLKP
                     WEPVRDESHCYPSGWQARLGMINEFHKQGSAFYFGLFDPDEKEIIGVANFSNVVRGSF
                     HACYLGYSIGQKWQGKGLMFEALSAAIRYMQRTQHIHRIMANYMPHNKRSGDLLARLG
                     FEKEGYAKDYLLIDGQWRDHVLTALTTPDWTPGR"
     gene            42143..43351
                     /gene="mdtH"
                     /locus_tag="J1Y83_000666"
     CDS             42143..43351
                     /gene="mdtH"
                     /locus_tag="J1Y83_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtH"
                     /protein_id="HBA7628850.1"
                     /translation="MSRVSQARNLGKYFLLIDNMLVVLGFFVVFPLISIRFVDQMGWA
                     AVMVGIALGLRQFIQQGLGIFGGAIADRFGAKPMIVTGMLMRAAGFATMGIAHEPWLL
                     WFSCLLSGLGGTLFDPPRSALVVKLIRPQQRGRFFSLLMMQDSAGAVIGALLGSWLLQ
                     YDFRLVCATGAVLFVLCAAFNAWLLPAWKLSTVRTPVREGMTRVMRDKRFVTYVLTLA
                     GYYMLAVQVMLMLPIMVNDVAGAPSAVKWMYAIEACLSLTLLYPIARWSEKHFRLEHR
                     LMAGLLIMSLSMMPVGMVSGLQQLFTLICLFYIGSIIAEPARETLSASLADARARGSY
                     MGFSRLGLAIGGAIGYIGGGWLFDLGKSAHQPELPWMMLGIIGIFTFLALGWQFSQKR
                     AARRLLERDA"
     gene            43415..44062
                     /gene="grxB"
                     /locus_tag="J1Y83_000667"
     CDS             43415..44062
                     /gene="grxB"
                     /locus_tag="J1Y83_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 2"
                     /protein_id="HBA7628851.1"
                     /translation="MKLYIYDHCPYCLKARMIFGLKNIPVELHVLLNDDAETPTRMVG
                     QKQVPILQKDDSRYMPESMDIVHYVDKLDGKPLLTGKRSPAIEEWLRKVNGYANKLLL
                     PRFAKSAFDEFSTPAARKYFVDKKEASAGNFADLLAHSDGLIKNISDDLRALDKLIVK
                     PNAVNGELSEDDIQLFPLLRNLTLVAGINWPSRVADYRDNMAKQTQINLLSSMAI"
     gene            44196..44756
                     /locus_tag="J1Y83_000668"
     CDS             44196..44756
                     /locus_tag="J1Y83_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HBA7628852.1"
                     /translation="MNKFLFAAALIVSGLLVGCNQLTQYTITEQEINQSLAKHNNFSK
                     DIGLPGVADAHIVLTNLTSQIGREEPNKVTLTGDANLDMNSLFGSQKATMKLKLKALP
                     VFDKEKGAIFLKEMEVVDATVQPEKMQTVMQTLLPYLNQALRNYYNQQPAYVLREDGS
                     QGEAMAKKLAKGIEVKPGEIVIPFTD"
     gene            44862..45908
                     /gene="pyrC"
                     /locus_tag="J1Y83_000669"
     CDS             44862..45908
                     /gene="pyrC"
                     /locus_tag="J1Y83_000669"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HBA7628853.1"
                     /translation="MTAPSQVLKIRRPDDWHLHLRDGDMLKTVVPCTSEIYGRAIVMP
                     NLAPPVTTVEAAVAYRQRILDAVPAGHDFTPLMTCYLTDSLDPNELERGFNEGVFTAA
                     KLYPANATTNSSHGVTSVDAIMPVLERMEKIGMPLLVHGEVTHADIDIFDREARFIES
                     VMEPLRQRLTALKVVFEHITTKDAADYVRDGNERLAATITPQHLMFNRNHMLVGGVRP
                     HLYCLPILKRNIHQHALRELVASGFNRVFLGTDSAPHARHRKESSCGCAGCFNAPTAL
                     GSYATVFEEMNALQHFEAFCSVNGPQFYGLPVNDTFIELVREEQQVAESIALTDDTLV
                     PFLAGETVRWSVKQ"
     gene            45982..46227
                     /gene="dinI"
                     /locus_tag="J1Y83_000670"
     CDS             45982..46227
                     /gene="dinI"
                     /locus_tag="J1Y83_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein I"
                     /protein_id="HBA7628854.1"
                     /translation="MRIEVTIAKTSPLPAGAIDALAGELSRRIQYAFPDNEGHVSVRY
                     AAANNLSVIGATKEDKQRISEILQETWESADDWFVSE"
BASE COUNT        11220 a        12541 c        11515 g        11113 t
ORIGIN      
        1 aacgcgtctt atcaggccta ccgctcgtac catttgtcca gggcggataa ggcgtttacg
       61 ccgcatccgc cagtacaagc cgattaatgc aacttcaaac gtggacggat aacgcggtta
      121 atactcccca ccagcatcat taatccggtt ttaaagtaac catgcagcgc aatctggtgc
      181 attcggtaca gcgagatgta tacaaagcgc gcaattcgtc cttcaatcat cattgagccg
      241 cgtgtcaggt tacccatcag gctaccgacg gtggaaaagt tcgacagcga taccagcgaa
      301 ccgtgatctt tatactgata atttttcagc ggcttaccgt tcatctgcgc cagaatgttg
      361 ttcattgcgc aggttgccat ctgatgtgcg gcctgagcgc gcggcggaac aaagccccct
      421 tccggacgcg ggcatgacgc gcagtcgcca atagcgtata tgtctggatc gcgagtggtt
      481 tgcagcgtcg gttccaccac cagctggttg atacggttgg tttcaagacc accgatatct
      541 ttcaggaagt ctggcgcttt gatcccggct gcccacacca tcagatcagc ctcaatatat
      601 tcgccatctt tagtgtgcag gccgccttca tcagcactgg tgaccatggt ttgcgtcagc
      661 acgcgaacgc caagtttcgt taactcacta tgggccgcag cagagatacg tggcggcaat
      721 gcaggcaaaa tacgttcgcc cgcttctacc agcgttacgt tcagggcttc gttggtcaga
      781 cctttataac cgtaactgtg cagttgcttg accgcgttgt gcaattcagc ggagagttct
      841 acacccgtcg cgccgccgcc gacaatcgca atgtttactt tgccattcgc gcccaggttg
      901 gcggagtatt tcaggaacag attcagcatc tcctggtgga agcgacgcgc ctggtgcggg
      961 ttatcgagga aaatgcagtt ctctttgaca cctggtgtat tgaaatcgtt agaggtgcta
     1021 cccagcgcca ttaccagggt gtcataggcg attttacgtt ccggaaccag cagttcacct
     1081 ttctcgtcgc gcagttctgc aatagtgatt gttttcgctt cacgatcaat atcaattacg
     1141 gaacccagct ggaactggaa accatgattg cgcgcatggg ccagatagct caacgcatcg
     1201 acgccttcat caagcgagcc agtcgccact tcgtgcagca gcggtttcca caggtggctg
     1261 tggttacgat cgaccagcgt aattttggct tttttcttgc gtcccagctt atgccccagc
     1321 tgtgttgcca tttccagccc accagcaccg ccgccgacaa tcacaatttt tttcaatggc
     1381 gtagtcaacg tgaccccctt aaatttatta accaattgtt aattaatggt tattaacata
     1441 gctcttaatt aacaacaggt tacagcaatg aaaatgttct tcgttattga gaataacatg
     1501 aatggtgcat tggtcatacc aaaattgatg ttaatcaagt tttgttgctg aaaagatagg
     1561 cgactttacc gggtgagatc aaaaaacaga gtttgttgtt tattatttga gctgatttgt
     1621 gagaatgata tagagtgagg tgtgtatgga gggcgttcgg taggcctgat aagaggcgca
     1681 tcaggcattg atgctcgact gtcggatgcg gcggaacgcc ttatccaacc cacagaatcc
     1741 ggagcgcatc aggtaccggc aaggttacct gatgcgacga gggcatttac ccgagggttt
     1801 tgaacgcttt aatgcgctgt aaatgcgggg agatattctt gaatttgtgc gtctgctctt
     1861 cgtcccagac aatctcgtaa taatgatgca actcttcaga tgtccgctgg ctgttaagcg
     1921 cttcatcatt acgcgacaaa atcaccaggc aacgatcgcg attcttctca cggaagttgg
     1981 tcacacactt agtggcaata tcggcatact cttccgggcg atcaatcttc ccttccatgt
     2041 tctcataagg gaacaaattg gggttgaaga tcacctggcg gatatcacag agaaaaccaa
     2101 tccgctcagc ccaataaccg cccagaccaa caccacaaat gagcgggcgc tcgtcgacgt
     2161 tcagttgcaa cattttgtcc acttctttga gcaaatgctg catatcatgt ttcggatgcc
     2221 gcgtactgta acttattagc cgtacatccg ggtcaataaa ctgcaattgt aagactttct
     2281 cgtggttacc cggactgtta gagtcaaaac cgtgcaaata gataatcatc gcttcctcac
     2341 ataagccagg gttaatgacc tgccttctct tcctgccagc gttcatgtaa ctgattcaga
     2401 cgggcgctga tcgctttcca gcgagccgag tccatcagtt cctgtcgcga aaatgaacct
     2461 ttatgataca aacgtgtaac acgttctgcc ttgatcggtg acagattatc taacacgctg
     2521 acggcccctt tacgattatt gcagaccagg atcatatcgc aacctgcatc cagtgatgcc
     2581 tgtccacgct cggcataact gcccataatc gctgcgcctt ccatcgataa atcgtcagag
     2641 aaaatcacgc cgtcaaaacc caattcctga cgcaaaacgg ttttcagcca gtagggagaa
     2701 ccgctcgccg gacgcggatc aacatcactg tagatcacat gcgcaggcat aatggcgtcg
     2761 agtttatttt cgcggattaa ggaactgaag accgacatat ctttagcgcg aatctccgct
     2821 tgcggacgtg ggtcgcacgg tgtttctttg tgtgagtctg ccgttactgc accgtgacct
     2881 gggaagtgtt tcccggtagt tttcattccg gcttcatgca taccatcaat aaaccggctg
     2941 gcaattgcca gggctttttg tggatcggca tgataagaac gctcgccaat cgccgcgctg
     3001 atatgcccga cgtccagcac tggcgcaaag ctgatatcaa tatccatagc aatcatttcg
     3061 ctggccatca accaacctgc ctcttgcgcc agttttccgc cttcttccat tcccaacagc
     3121 gcagcgaatg attgtgccgc tggcaagcgg gtaaaacctt cacgaaaacg ctgcacgcgt
     3181 ccaccttcct gatctaccgc caccaccaga tgattgcgcg atgctgcgcg gatctggcgc
     3241 accagttcac gtaactgggc aggatcatga tagtttcgcg taaagagaat cagccctccc
     3301 accagcggat gcgccagtat ttcacgctct tccgcgtcca gttcgtaacc ttcgacatcc
     3361 aacattactg gacccacact gctctcctta ttgttttatt aatagctgcc gccaggtgtc
     3421 atcggccagc ctgataaatt gttgatcgcc ggtttgtcgc cagcggtact caaaccaccc
     3481 tgctttgagc atcagcagcc agggaaacca tcgcctgacc tgacgccata attgcgccgg
     3541 ataaatcttc gcgcgagtgg catagtcatt gaccaattgc cgatgctggt cagtattttc
     3601 cacccacacc gccgccagtt ccagcgcgat atcaccatct ccggcatact cccagtcgat
     3661 gagttttaac cctgacgcgc tatgcactaa atttccggcg tggacatcca tatgcaatgg
     3721 acttaagcgt aaaagccgtg gttcccgcgc tttgcgcaga cgttttaaca ttcgcagcca
     3781 acccactgtc cgccgcgccg gatcgctttg ctgccagtac agttccagta acggcaacag
     3841 cgttattcgc cagccaaaac gcggttgttg atgtagataa tacagcaagc ctgccagttc
     3901 gttggtatcc ggcaaatacg tttttacctc gccgggcaga tagtcgacca ccatccagtc
     3961 acggagatat aaatgcggct tcggtgcaat gcatgcgggt agttgtgata aagcccgata
     4021 ctggcgcaag aacgcggact gcggcgcatc aggatcgtgc ggctgacgca caacaaaacg
     4081 ctggccctga tgttcaatga gaaaactccc gccactaagc cctcgattaa acgtcgtgac
     4141 gggatgaaac tgcgggaaaa agcgcgacag caattcgtcg cgcgtgaggg gattattgct
     4201 gcgaaacggc acctttacct gaccagataa tttcgcccgt ctgcaccagc atcagctgca
     4261 tttgtagggt cggagcgtta acgttgccag aggcgctgga gtacagcacg taatgagcgc
     4321 caacattgcg ggcaatgcct atggctttac tacgggtgcc taaactgtcc tgcggcgaca
     4381 aacctaattg ttgcttagcc atcgacagct gctgggcgga aaccagggta aatttaccgt
     4441 tattagccag cgcatttcgc agcgtttcgg tcgcttctgc ggcattcaac gaaccgttag
     4501 tacggttgtt aacgctatca accagcagga cgctacccgc agtcacgccg tcagccccaa
     4561 gcatcttact gaccatcggc tgcattgcgc cattccagtc ataatggcga atatgcggcg
     4621 caggcggtgc agtttgatct tcgtgctcaa ttgggcctgg ctgctgcggt atcgtcggca
     4681 ccgatggcac tgtggggaca ggctgttgtg gctcagcagg ttgttccggc gctggtttca
     4741 cttcttctac cggtgcaggt tcacgttgcc ccacacaccc ggcaagaaac atcgccagcg
     4801 cggtaatcaa ggcgtagcga ctcatttttg tcatcaagat tcacccctta caaatataga
     4861 taaagtctga ctttgtgcgc ccccagaaaa ttggcgctgc cgtacagcgt gaccgccgaa
     4921 tgtgcgggaa tggtaacgct gcgtggcctt tccagaggat gcatctccag ccctctggcg
     4981 tcataccagt aaaaacgata atgaacggta acgggttctt gcctttcgtt ataaagcgtt
     5041 gaggatgctg aaggttgaat atcagatgtc gaaaggacgg gcttttctgc actgatgccc
     5101 gcagccaata aagatgactc catcaccagc gattgctcat cattcaccgg aatttccgga
     5161 tgtgaacgac agcccaccag cactaacaac gccagagaca tcagcccaaa gcatcctttt
     5221 ctcatcgtta cagaccttta tgcgccagca ttggtcccag cggacggcca cccaacaaat
     5281 gcatatggat gtggtaaacc tcttgtccgc catggcggtt ggtgttcatg atcagacgat
     5341 agccatcttc ggcaatacct tcttgctcag caatttttgc cgctacggtg atcatgcgtc
     5401 ccagcgcctg ctcatgctca gctgacacgt cgttcacagt cggaatgagg atattcggaa
     5461 tgatcagaat atgcgttggc gcttgcggcg aaatatcgcg aaacgccgtt accagatcat
     5521 cctggtagac gatatcggag gggatctcac gacgaataat tttgctgaat atagtttctt
     5581 ctgccacgac attttccttt ttcaaatagc catgcgttgc gccatgctac gccgcgccac
     5641 actgcgagta tagagtatgg tcgagttaaa cctgctcttt caaccttaac tcacgatttc
     5701 ttaagcaaaa aatcgttttg ctgccgtata tatcgccatt attcccattt ctgcctgcga
     5761 atgcgctgaa aagacattct ctgtttggtt tacactccaa atatgaatgc gtatatttct
     5821 catttgcatt tacacacaaa aattattcgc acactaaata taaccgctca tcaccctgct
     5881 tcggcatgag cctgacgcca acataaacca agagatttca aatgctttcg acacaattta
     5941 acagggataa tcaacatcaa gccatcatca aaccgtcact ccttgccggt tgcatagcac
     6001 tggcactatt accttccgcc gcttttgctg caccagccac tgaagaaacg gtgattgttg
     6061 agggttcagc cacagcccca gatgatggcg aaaatgatta caacgtaacg tctacctctg
     6121 cgggtaccaa aatgcagatg actcaacgtg atattcctca gtcggtcact attgttagcc
     6181 agcagcggat ggaagatcag cagttacaaa cgctgggcga agtgatggaa aacacgctgg
     6241 ggatcagcaa aagtcaggcg gattccgatc gtgctcttta ttattcccgc ggattccaga
     6301 tcgataacta tatggttgat ggtatcccca cctattttga atcgcgctgg aatctgggcg
     6361 acgcactttc tgatatggca ctgtttgaac gcgtagaagt agtgcgtggc gcgacaggcc
     6421 tcatgaccgg gacgggtaat ccatctgcgg caattaatat ggttcgaaaa cacgcgacca
     6481 gtcgtgaatt taaaggcgat gtctcggcgg aatacggtag ctggaacaaa gaacggtatg
     6541 tggcggattt acaaagccca ctcaccgaag acggtaaaat ccgcgcacga attgtcggcg
     6601 gctaccagaa taacgactca tggctggacc gctacaacag tgaaaagacc ttcttctccg
     6661 gcattgtcga tgctgattta ggcgatctta cgatgctttc agccggttac gaatatcagc
     6721 gtattgatgt taatagccca acctggggcg gtttaccgcg ctggaatact gatggcagca
     6781 gcaacagtta cgatcgcgca cgcagtaccg cacctgactg ggcgtacaac gataaagaga
     6841 tcaacaaggt ctttatgacc ctgaagcagc ggtttgctga tacctggcaa gcgacgctga
     6901 atgccaccca ctctgaagtc gaatttgaca gcaaaatgat gtatgtcgat gcctatgtaa
     6961 acaaagcgga tggtatgctg gttgggccat acagtaatta tggacctggt tttgattatg
     7021 tcggcggcac cggttggaac agcggcaaac gtaaagttga tgcgctggat ttgttcgctg
     7081 acggtagtta tgaattgttt ggtcgtcagc acaatctaat gtttggtggc agttacagca
     7141 aacaaaacaa tcgttacttc agttcatggg ccaacatctt cccggatgaa attggcagtt
     7201 tctacaactt taatggcaat ttcccacaaa ccgactggtc accacagagc ctggcgcagg
     7261 acgataccac acatatgaaa tcgttatatg ctgccactcg tgtcaccctt gccgatccgc
     7321 tgcatctgat cctcggcgca cgttatacca actggcgggt tgatacgctg acttacagca
     7381 tggagaaaaa ccacaccacg ccttacgctg gtctggtgtt tgacatcaat gacaactggt
     7441 cgacctacgc cagctatacc tctattttcc agccgcaaaa tgatcgtgac agttcaggca
     7501 aatatctgac tccaatcacc ggtaacaact acgagctggg tctgaaatcg gactggatga
     7561 atagccgtct gaccaccacg ttagccatct tccgtattga gcaggataat gtcgctcagt
     7621 ccaccggtac acctatcccc ggcagcaacg gcgaaaccgc ctataaagcg gtggatggga
     7681 cagtcagtaa aggtgtggaa tttgaactca acggcgcaat taccgacaac tggcagttga
     7741 cgtttggcgc aacgcgctat attgccgagg ataacgaagg aaacgccgtt aatcctaatc
     7801 tgccacgcac cacggttaaa atgttcacca gctatcggtt gcctgtcatg ccagagttga
     7861 cggtcggcgg tggtgttaac tggcaaaatc gcgtgtatac cgacaccgtg acaccgtatg
     7921 gcaccttccg cgccgagcaa ggtagctacg cgctggtgga tctcttcacc cgctaccagg
     7981 tgacgaaaaa cttctcgtta caggggaatg tcaataacct gttcgacaaa acctacgata
     8041 ccaacgtgga aggttctatc gtctacggcg caccgcgtaa tttcagcatt accggcacgt
     8101 atcaattctg aataacgcct gtaaaaaagg cagccatctg gctgccttag tctccccaac
     8161 gtcttacgga ttagtggtta cggatgtact catccatctc ggttttcagg ttatcggatt
     8221 tagtaccgaa aatcgcctga acaccagaac cagcaaccac tacgcccgct gcgcccagtt
     8281 tcttcaggcc agcctgatcc actttagaca catcagcaac gctgacgcgc agacgggtaa
     8341 tacatgcgtc gaggttagta atgttttctt taccaccaaa tgcagcaacc agagccggtg
     8401 ccatttcgct ggtacctgtc gcttttgcat cttcagtcgc gtcttcacga cccggcgttt
     8461 tcagatccag tgctttaatc agcacgcgga agatggtgta gtaaacaatg gcataaccga
     8521 taccgacgat cgggaacagc cacagtttgc tgctgttacc agacagaacg atgaagtcga
     8581 tcagaccgtg cgagaacgac gtaccgtcac gcatccccag aagaatacag attgggaatg
     8641 ccaggcctgc cagaatcgcg tggatgatgt acaggatcgg cgcaacgaac atgaaggaga
     8701 actcgatcgg ctcggtgata ccggtcagga acgaggtcag cgccgcggag atcataatac
     8761 cgcccacttt cgcgcggttt tctggtttag cagagtgcca gatagcaatt gcggcagctg
     8821 gcagaccgta cattttgaac aggaagccac cagacagttt acccgcagtc gggtcacccg
     8881 ccatataacg cggaatgtcg ccgtggaaaa cctgacctgc tgcgttggtg tattcaccaa
     8941 tttgcatctg gaaaggtacg ttccagatgt ggtgcagacc aaacggtacc aggcaacgtt
     9001 cgatgaaacc gtaaatgcca aacgcaacta ccgggttctg gtaagcagcc cactgagaga
     9061 aggtctggat tgcagaacca atcggcggcc aaatgaagga cagcacaacg ccagtaaaga
     9121 tggcagccag gccagaaatg atcggcacaa agcgtttacc ggcaaagaag ccaagatact
     9181 caggcagctt aatacggtag aaacggttaa acatatacgc tgcaatcgca ccggagataa
     9241 tccctccgag tacgccagta tccgccaggt gtttagaggc gatttcttca gcaggtaaat
     9301 gcagtaccag tggcgcaacc acggccatgg ttttaaccat gatgccatag gcaacaactg
     9361 cggccagcgc ggatacgcca tcgttattgg taaagccgag ggcgacaccg atcgcaaaaa
     9421 tcagtggcat gtttgcaaag acggaaccgc ctgcttctgc cataacatgc gatacaacgg
     9481 cgggcagcca gctgaaattc gcggaaccga cgcccagcag aatacctgcg ataggcagta
     9541 cggataccgg cagcatcagc gatttaccga ccttttgcag gttagcaaat gcattcttaa
     9601 acataattga gagtgctcct gagtatgggt gcttttttta cgttctcacg cgtggcaagg
     9661 ggggagtgcc tcgccgtgta cagggcatct aagcgccctt tatttattac acagagtaaa
     9721 ataattcagt gccgatttgt ttgacggcta tcacgtttca acttcagatc attggagaag
     9781 ttgcacattt tgacaaaaca tatgtcacaa tatttcaaac gtggttattc accgcccgat
     9841 tttcggcggt gaactttact cgttttagcc attaattacg agctttaaaa aaactcattc
     9901 aacggatgga ttgaaggcgg gaagcgtcga tgtgaaacag acgggcgaag ttatcggtgg
     9961 ttacctgcgc cagttcttca acggcaacac ctttcaacac agccatgtat tctgcaacgt
    10021 cacgaaccat cgcaggttga ttctcttttc ctcgatgcgg tactggcgca aggtaaggtg
    10081 agtcagtttc caccagtaac cgatccaggg ggacataacg cgcagcatcg cgcagttgct
    10141 ccgcattacg gaaggtcaca atgccggaaa aggagatgta aaatccgaga tccagtaatt
    10201 tgcccgccgt ttctctgtcc tctgtaaaac agtgtagtac gccaccgcaa tccgtcactt
    10261 tttcttcgcg cagaattgcc agcgtatcgg cgcgggcgtc acgggtgtgg acgataaccg
    10321 gcttgttcag ttcacgacca atctggatat gatggatgaa ggactcttgc tgacgtactt
    10381 tagtttccgg cgtgtaataa taatccagcc cggtttcacc cagcgctaca acgccctctt
    10441 ctgccgccag acgacgtaaa tcttctacat cgtagggatc attctggttg agtggatgca
    10501 cgccacaaga aaatacgacg ttgtcgcgtt cgcctaccag atcccgcatg tgtaaataac
    10561 ccggtaatgt tgtggcgact gccagacaaa atttcacatc gcgtgcggcg gctttcgcca
    10621 gaacgtcatc cacgtcctta tgcaaagatt cataatccag accatcgaga tggcagtgtg
    10681 agtcgactaa aaacatgatg tatctcttaa agatgaggaa ccggtagcac aacgcccggt
    10741 tgcaggtaat gctcaatacg cagtaaaaga tcggtgatga gaagctcgcg gttgatgcct
    10801 gtgacagaca ttaactgttc acgaatgtgg caaacatccc ccagtatagc ctgcaggcgc
    10861 gagggagaaa gatggtttgc cagttcgacg accagccccg gcacatcaac attggtcacc
    10921 tgcgcagcgc cataatggcg ttttagcgca tccatcagca acgttgccag ccagtgtaaa
    10981 cgcgccggag cttgttcatg attaagggcc gctagcagcg aataccagtc gcccgattgc
    11041 acgctatatg ccagcgcctg acacaatgtt tcacgagcct gccagttatc tccctgaaac
    11101 aacgccagtg ccgccccagg cgaaccggcg cttaagcgca atgcggcaag tagtgcatcc
    11161 tgtgacattg tcacttcgcg tgaaagccag gtcacggcgt actgttccgg cggcggcgca
    11221 aggtaatgta accgacaacg actacgtaat gttgccagta aacgttctgg ctcgcgggta
    11281 gcaaggaaaa accacgtctc tgctggtggc tcttcaagcg ttttcagcaa tgcgttagcc
    11341 gcggcgtcgg ttagtaaggc agcatcggtt acccagacaa ctttcgcacc acctaagcgt
    11401 gcgtgctcat tcagcttttc ggtgacctca cgtaccgcat caatgcccag cgcatttttc
    11461 cctttttcgg gagccagggt gtagtaatcg ggatgtgtgc cagcctgcat caactgacat
    11521 ccacgacagt gaccgcaact tttgtggccc tgcggttgtt ggcagagtaa ataacggctc
    11581 agggcgtaga ttaaagcatc atcgcccatg cccggtaacg cctgaatgag tagcgcatgg
    11641 tgacctcttc cggcctgata gctggctacc agtttttcga aatcaggtcg taaccatgga
    11701 taccatctca tgcgtccaac tccttcaccc agtgggtcac ggtagtgcgg attgcaccca
    11761 tcacggcctc cagcggctgg gtggcatcaa tggtatgaat gcttttatct tgtgctgcca
    11821 attccagata gcgggcgcgg gtgcgattaa agaaatcgaa agactcttgc tcaatacgat
    11881 ccagctcgcc gcgcgcacgc gcgcgtttta agccaacttc cggggtaaca tcgagataga
    11941 gcgttaagtc ggggcgaaaa tccccgagaa cagcatcacg cagtgttgcc agcatatgtt
    12001 gatcaatacc acgtccgccg ccctgatacg cctgagtgga gagatcgtgg cgatcgccaa
    12061 tcacccaggt accgttagcc agcgcaggtt tgatgaccgt ttctaccagt tgaacacgcg
    12121 cggcataaaa catcagaact tcggctttat cggtaatgac ttcatcgcct accgatttga
    12181 tatccagcac caggcttctt aacttttcgg caagttgcgt accgccaggt tcccgagtga
    12241 aaaccatgtc gcggataccc agttgctcaa gcgtctcaac caccacgtta cgcgcggtag
    12301 ttttgcctgc gccttccagc ccctcaatga cgatatactt actgcgcatt tttttcctta
    12361 agcactttca gataatcctg cacagacttg ttatgactgg caagattggt attaaacgtg
    12421 tgaccacctt taccatcggc cacaaaatag agatacggcg tttttgccgg atgcgcagca
    12481 gccttcagcg aatccgcccc cggcgtcgct atcgcacctg gcggcagacc ggtaatggta
    12541 taggtgttat acgctgtcgg cgtttccagg tctgcacgag aaagtttgcc attataacgc
    12601 tctcccatcc cgtaaatcac ggtcgggtcg gtctgcaggc gcataccaat gcgtaaacgg
    12661 ttgataaata ctgaggcaac ctgatcgcgt tcactggcaa cggcggtttc tttttcgata
    12721 attgatgcca tcgtcaccaa ctggttttta tctttataag gcagaccgtc cgcacgccct
    12781 tcccaggcgc tatcgaccgc tttcaccatt ttcttgtgcg ctcgcttgag taacgcgaca
    12841 tcggtggtat tggcggtata catccaggtg tctggccaga accaaccttc aatccactcc
    12901 gggttttcca gttcaagtgc ctgcgctacg gtggcgtact tatcgtcgct cagcgtgtgc
    12961 ttgatatacg gagcctcacg caattgcttg aggtagtcgc tcagacgcat cccttctacc
    13021 agtcgcagcg ggaactgtgc ttctttaccg ctttccaaca atttcagcat ctcgcgcacg
    13081 gtcatctgcg gtgtaaagcg gtaagtcccg gctttaaagt gagaaagatc cggttcgata
    13141 cgcagcaacc attgaaaaac ccgtgggcga ttgatgatct tatcggcata aagctgttca
    13201 ccgagcgcca gacgtccggt cccaggcttc agggtaaata tcgtctcttc tttgataagc
    13261 aatttgctgt cggcaagatg gcgaaccttc cagacgccca caccagcggc gatacccagt
    13321 accaccagca ataacaagat tatcaataac acttttttca tgactaattc gggcgctcac
    13381 aaagtggggc taaatattca tataacgttg ctgacgaaaa ggagacatcg ccacaggcac
    13441 gtacgggcat cactggcatt aacgcattac aaataaccat ctcatctgcc tgcaacgcct
    13501 cttccagaga ggcttgcact tcgacaagct gataagagga ttgtgccagc aaacggatac
    13561 agaattgtcg cataatgccg ttaacacctg cctgatccag tcgcggcgta taaactacgt
    13621 tgcccttccg ccagaacaaa ttagccgcac agcattccgt aacccaccct tcgctgtcaa
    13681 ggaccagcgc ctcatcagcg tttgtctgct caagatgaga gcggatcaat acttgctcaa
    13741 gccgattaag atgtttaata cctgcaagat gaggattgcg ccccagccgc accgggctta
    13801 gcgccaacgt catcccttcg ttacgcaaac ggtcgtaatg tgcaggataa gccgtaacgg
    13861 agagaacccg cgttgccggt ccactgttca atgtgctgta ccctcgcccg ccactaccgc
    13921 gactgatcac gactttcagt acaccatttt gctgttctgc tgccagcgtt ttcatctctt
    13981 gttcaagctg aggccagaag tcacaggaaa tcatcaaccg ctgacaagca tcctgtagtc
    14041 gctggatatg cgccgataac aaactgactt taccgtcgat aactctggcg gtggtaaaac
    14101 aaccatcacc aaactgcgtt gcccgatcgc ttactgccag cgattcctgc ttatgaccgt
    14161 taattaagaa cataccggct ccttatcaga gaattgttag tgtggcagca tgttcactac
    14221 ggaacaagtc ggaataaaag ctaagaaaaa aggcccgcaa gcggaccttt tataagggtg
    14281 gaaaatgaca atttagatct ttttaaagat caaagaacca ttagtgccac cgaagccgaa
    14341 ggagttacac agagtgtatt ccattccgct aacctgacgc gcttcgtgcg gtacgaaatc
    14401 caaatcgcaa ccttcatccg ggttatccag gttgatggtt ggcggaacag cctgatcgcg
    14461 cagcgccagg atggagtaga tagattctac tgcacccgcc gcacctaaca ggtgaccggt
    14521 catagatttc gtggagctta ccaacacgcg gcttgcagct tcaccgaaga tggttttcac
    14581 cgcctgcgct tcagctttat cgccagccgg cgtagaagta ccgtgcgcgt taacgtagcc
    14641 aatctgactc gcttcaatgc ctgcatcacg cagagcattt gccatcgcca gagctgcgcc
    14701 tgcgccattt tctggcggtg acgtcatatg ataagcatcg ctgctcatac caaagccgac
    14761 gagttcagcg taaattttcg caccgcgttt tttcgcgtgt tcgtactctt caagtaccag
    14821 cataccggca ccatcgccca gtacgaaacc atcacgctct ttatcccacg ggcggctcgc
    14881 cgcttgcggg ttatcattgc gggtagataa tgcacgtgcc gcgccaaaac caccaacgcc
    14941 cagcggcgta ctggctttct ctgcgccacc tgcaaccatc acgtcagcat cgccatacgc
    15001 gataatacgc gcagcatggc caatgttgtg cacgccggaa gtacaggcgg tcgcgataga
    15061 gatgcttggg ccacgcaggc catacatgat agtcagatga cctgccacca tgttcacaat
    15121 cgttgacgga acgaagaatg ggctgatctt acgtggacca ccgttcatca gagatgtgtg
    15181 gttttcttcg atcagtccga ggccgccaat cccggagcca attgcggcac caatgcgggt
    15241 tgcgttctct tccgttattt caaggccaga atcctgcatg gcctgaacgc cagcgacaat
    15301 tccatattga atgaaggcat ccatcttgcg ctgttctttg cgcgagataa tgtcctcaca
    15361 gttaaaatcc tttactaagc cagcaaattt cgttgcatag gcgctagtat cgaaatggtc
    15421 gattaggctg atgccactct gaccggcaag cagagctttc caggtagact ctacggtatt
    15481 gccgacagga gacaacatgc ccagtccggt cacaactaca cgacgcttag acacgtttgt
    15541 cctccaggga gggaaaaaat gattctagtg ggacaaaaag ataaaactca ggcggtcgaa
    15601 cgaccgcctg gagatgttca cttacgcctg gtggccgttg atgtaatcaa tggcagcctg
    15661 aacggtggtg attttctcag cttcttcgtc cggaatctca gtatcaaact cttcttccag
    15721 agccattacc agctcaacgg tgtcaagaga atccgcgccc aggtcttcaa cgaaagaagc
    15781 attgttggta acttcttcct gcttaacgcc cagctgttcg ccgataattt tcttaacgcg
    15841 ttcttcgata gtgctcatac tcttaaattt cctatcaaaa ctcgctttcg cgatggtttt
    15901 cgtagtgtat aaaatgttga aaaatttgca actaaatccc ggcaggtctt accacgattt
    15961 tacgttattt tgaggcctta ccccccaata acgcaaatca ttttgcacta attattactc
    16021 ataaccacgc agactgcgcg caacatgagc attttgtgca aatcgcggtc agaccatgta
    16081 catcccgccg ttcacatgca aagtttcacc cgtgatgtaa gctgcttcgt cggatgccag
    16141 gaatgcaacc gcgttggcga tttcctgtgc gccgccgagg cgacccgcag gaacctgcgc
    16201 caggataccc gcacgctggt catcgctcag cgcacgtgtc atgtccgttt caataaagcc
    16261 cggagcaaca acgtttacag taataccgcg tgacgcaact tcgcgcgcca gtgatttact
    16321 gaagccgatc aagcccgctt tcgccgcagc gtagttggcc tgaccgccat ttcccatggt
    16381 accaaccaca gaaccgatag tgataatacg accatgacgc tttttcatca tagcgcgcat
    16441 taccgctttt gacagacgga aaacagatga aaggttggtt tcgataatat cgttccactc
    16501 ttcatctttc attcgcatta acaggttatc acgagtgata ccggcattat tgaccaggat
    16561 atccacttca ccaaattctg cgcgaatttt ttccagaaca gattcgatag atgccgggtc
    16621 ggtcacattc aacatcagac ctttgccgtt ggcacctaaa taatcactga tcgcctgagc
    16681 gccattttca ctggtcgcag tgccaataac tttcgcgcca cgggctgcga gcgtttcagc
    16741 aattgcgcgg ccaattccgc ggcttgcacc ggttaccagt gcgatttttc cttcaaaatt
    16801 catgattttc ctcttttaaa gctcgagcgc cgctgccatc gctgaaggtt cgttcagcgc
    16861 cgaggcggtc agggtgtcga caatgcgttt cgtcaggcca gtaagcactt tgcccgggcc
    16921 gacttcatag agatgttcta cgccttgcgc tgccatgtac tcaacagact tcgtccactg
    16981 aaccgggtta tacaactgac gtaccagtgc gtcacggatg gcatcaccat tggtttcgca
    17041 tttcacatca acgttattca caacaggaac tgttggtgcg ttaaaggtga ttttcgctaa
    17101 ttctactgcc agtttgtcgg ctgctggttt catcagcgca cagtgagacg gtacgctcac
    17161 tggtaacggc agagcgcgtt ttgcgcccgc agctttacag gcagcgccag cacgctcaac
    17221 cgcttcttta tgaccggcaa taaccacctg tcccggagag ttaaagttta ccggagaaac
    17281 gacctgacct tctgcagctt cttcacacgc ttttccaata gacgcatcat ccagaccgat
    17341 gattgccgcc atagcgcccg ttccttccgg tacggcttct tgcatgaact tgccgcgcat
    17401 ctcaaccaga cgcaccgcat cagcgaaatc aatcacacca gcgcaaacca gcgcggagta
    17461 ttcccccagg ctgtgaccgg ccatcattgc cggtgcttta ccgccctgct gctgccatac
    17521 gcgatacagc gcaacagatg cagtcaacag cgctggctga gtttgccagg ttttattcag
    17581 ttcttcagct ggcccctgct gggtcagcgc ccacagatcg tagcccagcg ccgcagaagc
    17641 ttctgcaaac gtttcttcga caattggata gctcgccgcc atatcagcca gcattccaac
    17701 ggtttgagaa ccctgtccag ggaacacaaa tgcaaattgc gtcatgtttt aatccttatc
    17761 ctagaaacga accagcgcgg agccccaggt gaatccaccg ccaaaggctt caagcagaac
    17821 caactgtccc ggcttaatgc gcccgtcacg tacagcttca tccagcgcgc acgggacaga
    17881 ggccgcagag gtattaccgt ggcgatccag cgtcaccacg acattgtcca tcgacatgcc
    17941 gagttttttc gccgttgcac tgataatacg caggttagcc tgatgcggaa ccagccagtc
    18001 cagttgagaa cggtcaagat tattcgccgc aagcgtctca tcaacgatgt gcgccagttc
    18061 cgttaccgca accttgaaga cttcgttgcc cgccatcgtc agatgaattg aattctctgg
    18121 attcacacgg tcagcattag gcagcgtcag caattcacca tagctaccgt cggcatgcag
    18181 atgggtggag atgattcctg gctcttcaga ggcagccagc accgctgcgc ccgcgccatc
    18241 gccaaaaata ataatagtcc cacgatctgt tggatcgcag gtgcgcgcca gtacatcgga
    18301 accgacgacc agagcatact tcaccgcccc agatttcacg tattgatcgg ctacgcttaa
    18361 tgcataggtg aaacctgcgc aggctgctgc aacgtcaaat gccgggcaac ctttaatgcc
    18421 caacatgctt tgaatctgac aagctgcgct cgggaaagcg tgcgtcgcag aagtcgttgc
    18481 cacaacgatc aggccaatct ggtctttctc aatacccgcc atctcaattg cgcgtgtcgc
    18541 cgcttcaaag cccatggttg aaacggtttc gtttggcgcg gcaatgtggc gttcgcggat
    18601 accggtacga gtgacaatcc actcgtcaga ggtatccacc attttttcca aatcggcgtt
    18661 tgtccgcact tgttcgggca gatagctgcc agtaccaata atcttcgtat acatgtacgc
    18721 tcagtcactt ttcggttata taccgtcact tgcaaactgc gagttcgctg gcagcgtcct
    18781 gctaccgcag agttccgctt ttgccaccgt ccagcagctc aaaaccagct gggtatacag
    18841 attccaggcg agcggcaatt cgctgaggaa cttgtcgctg caccgcctgc actgcctgtt
    18901 caatcgcgac cgcaaaagct cgctgattgg ctgcaccatg actttttatc accgtgccgc
    18961 gcaatcctaa cagacaggcg ccgttatact ggtcggggtt gaggtgactg aatcgcctcg
    19021 tcaggctctt ttgtagccaa cgctttaata acagtagcca ccacgaccgt tttttccctt
    19081 caccctgaga tttcagcaga gaaaggaaca tcctgacaac accttccatc gtctttaatg
    19141 tgacatttcc tgtaaagccg tcacaaacca gcacatctgt cttgccagtt aacaactcat
    19201 tggcttcaag atagccgata taattgatag aagggattgt tttaagcacc gctgaggcat
    19261 cccgaatact gtcgagaccc tttacttctt cttcaccaat attgagcaac gccacgcgag
    19321 gattaggaat ttccaccacc tcttcagcca gaactgagcc cataatggca aattgcacca
    19381 acattgtact gtcacaatcg acgttggccc ctaaatcgag gaccaccgtt ttgccctttt
    19441 gctgatgcgg taataccgtc accagcgccg gacgctcaat cccctccagg ggcttgagta
    19501 ataattttgc cagccccatt agcgccccgg tattaccggc actgacacag gcttgcgctc
    19561 gaccttcttt caccagctcc agggccacgc gcattgaact cccacgactg gcgcggatag
    19621 cttgcgaagg ccgggcatca ctggcgataa ctgactgcgc aggaataatc tgcagacgcg
    19681 aacgttgttc aaagtcagct ttagcaagta atggcgtgat ggcgtcggga ttgccgacta
    19741 aaagaagagt gagttgcgaa ttagaattca gtgcctgcaa tgctgcaggc actgtcacgg
    19801 aagggccaaa atcccctccc atgacatcta acgccagggt tagacgtgtc aaggtgtcgt
    19861 cgccgcccgg tttggtcttt ccccagttgc ctggggaaaa tcctcactaa gcttcatcac
    19921 gcagacgcgt gattacttag cgatgacctt gcggccgcgg tagtaaccgt cggcagtgat
    19981 gtggtgacgc aggtgttttt caccagaagt tttgtctaca gacaggctgg tgactgcggt
    20041 cagcgcgtca tgggaacgac gcatgccacg tttggaacgg gttggtttat tctgttgtac
    20101 ggccatggac cttactcctc aattacggtt tatccccgat ccaacgggga gcaccaatta
    20161 cttacgcttt aagctggcta atacggcaaa tgggtttggc ttttgcgctt cttcaggcag
    20221 ttcaccaaag accatgtccg cttcggacac ttcacagtgt tcagaatcat gcaccggaac
    20281 taccggcaag gcgaggatga tttcatcttc aaccattgca agcagatcga tttcaccgaa
    20341 ttcgttaacc tcaatcggtt catacgcttc cggcagtgct tcagcctgtt cgtctgaacg
    20401 cacaggacta aaacaatacg ttgtgtagac ctgatgagta aacggcttcc cgcaacgctg
    20461 acactcgagc gttaccgtca ccttcgcatc gccgtttaac actgcgagac gttggttatc
    20521 gatagcgaac gacatggagc attccacatc actgtccaca ctgactacgg attcggcgac
    20581 gcgctcaacc tgatcaggag tatagatacc ctggtaatca aggcgttttt gagccgtacg
    20641 aaccggatcg agagtcaggg gtaattttac cttttgcata gggcgcgcat attaactttg
    20701 taacgtcata gagtcaaaga aaaaggcagc ctgtggttgc cttttgccaa taattcgcac
    20761 acattgcggg ttactgcttt attttcgttc agtgctgata acgaacatca acctgcaaaa
    20821 cgaagcgcat agtttaaaat ggctatcatc aatacgctat atctgatgga aaaaaatatg
    20881 cctaaactta ttttagcctc cacctcgccc tggcgtcgcg ctcttctgga aaaactgcaa
    20941 atctcttttg aatgtgcagc acccgaggtc gacgaaactc cacgcagcga cgaatcaccg
    21001 cgacagttgg tgcttcgact ggcacaagaa aaagcgcaat ctctggcgtc acgttatccg
    21061 gatcatttaa ttattggttc agaccaggta tgtgttcttg atggcgaaat caccggtaaa
    21121 ccgttaacgg aagaaaatgc ccgtctgcaa ttacgcaaag ccagcggcaa tatcgtcacc
    21181 ttctatactg gactggcgtt gtttaattcg gcgaatgggc atctacaaac agaagtggag
    21241 ccttttgacg tccatttccg tcacctgagc gaggcggaga ttgataatta cgtgcgtaaa
    21301 gagcatcccc tgcactgcgc gggtagcttt aagagtgaag gatttggcat tacgctgttt
    21361 gagcgtttag aggggcgtga tcctaacacg ctggttggtt taccgctaat cgcgctgtgt
    21421 cagatgttac gtcgggaaga gaaaaacccg ctgatgggat agttttttgc gccggatgcg
    21481 gttacttcgc atccggcaat cggtgctggc ggcctgatgc ggcgtaaacg ccttatcagg
    21541 ccaaccaccc attatcaggt ttatatgctt gtattagcgc gcgttacgca gcttttgcaa
    21601 acaacgcttc aaaccttcat ccatcggcgc ttcaatacgc atcacctcac cggtccccgg
    21661 atgagtaaac ttcaacgctg cggcgtgcag gaacaggcga tttaatcccg tgcctgcttc
    21721 agtgagctgt ctgtcaaatt cacggtcacc gtagcgatcg tcaaaggcaa tcggatgacc
    21781 cgcatattgt gtatgcacac ggatctgatg agtacgccct gttaccgggc tacaacgcac
    21841 cagggtggca aatgcatagc gctcttccac tttaaagcgt gtttccgacg gtttgccttc
    21901 ctgactcaca cgcacgatac gttcgccgct ttgcagaata tttttcagta acggcgcttg
    21961 aacgctcttc acatgcgact gccactgacc gcgcaccagc gccaggtaat ctttttgcat
    22021 ccctttttca cgtaattgct catgcagaga acgcaacgcc gagcgttttt tcgctaccag
    22081 caacacacct gaggtgtccc ggtcaagacg atgaaccagt tcaaggaacc gcgcttccgg
    22141 gcgcaacgcc cgcaaacctt caataacgcc gaagcttaaa ccactgccgc catgtaccgc
    22201 cgtaccggaa ggtttattca gcaccaggat atgatcatct tcatataaga tgacgtccgc
    22261 cagcgccgcc accttttgca gatgtggcga aaccgcctct tcttcccgct cagcaacgcg
    22321 aaccggtggg atacgcacct catcacccgc ttcgagttta tattcaggct taatacgttt
    22381 tttgttcacc cgcacttcgc ctttacgcaa aatacggtaa atcatacttt ttggtacgcc
    22441 tttcaattgg gtacgcaaaa agttgtcgat acgttgcccc gcttcgtcag cggtgatagc
    22501 aacaattttt acggatggag tctctgtttt catggtgggc gattctaatt agccaacagg
    22561 attcgcgcca ctcatttttc tatgcttata tttagttttg caccttatta cttcactagg
    22621 tgatcacttt attgatggtt attaaaccaa tcaccagcaa gaagtgaaaa aactgtgagt
    22681 aagcgggtga taaatggtaa aagtcatctt gctataacaa ggcttgcagt ggaataatga
    22741 ggccgtttcc gtgtccatcc ttgttaaaac aagaaatttt acggaataac ccattttgcc
    22801 cgaccgatca tccacgcagc aatggcgtaa gacgtattga tctttcaggc agttagcggg
    22861 ctgcgggttg cagtccttac cggtagatgg aaatatttct ggagagtaat acccagtctg
    22921 tttctctgat aattgcgctg tttttccgca tgaaaaacgg gcaaccgaca ctctgcgcct
    22981 ctttgagctg acgataaccg tgaggttggc gacgcgacta gacacgaggc catcggttca
    23041 cacccggaaa ggcgttactt tgcccgcagc ttagtcgtca atgtaagaat aatgagtaag
    23101 ttacgatgaa aagaatgtta atcaacgcaa ctcagcagga agagttgcgc gttgcccttg
    23161 tagatgggca gcgtctgtat gacctggata tcgaaagtcc agggcacgag cagaaaaagg
    23221 caaacatcta caaaggtaaa atcacccgca ttgaaccgag tctggaagct gcttttgttg
    23281 attacggcgc tgaacgtcac ggtttcctcc cactaaaaga aattgcccgc gaatatttcc
    23341 ctgctaacta cagtgctcat ggtcgtccca acattaaaga tgtgttgcgt gaaggtcagg
    23401 aagtcattgt tcagatcgat aaagaagagc gcggcaacaa aggcgcggca ttaaccacct
    23461 ttatcagtct ggcgggtagc tatctggttc tgatgccgaa caacccgcgc gcgggtggta
    23521 tttctcgtcg tatcgaaggc gacgaccgta ccgaattaaa agaagcactg gcaagccttg
    23581 aactgccgga aggcatgggg cttatcgtgc gcaccgctgg cgtcggcaaa tctgctgagg
    23641 cgctgcaatg ggatttaagc ttccgtctga aacactggga agccatcaaa aaagccgctg
    23701 aaagccgccc ggctccgttc ctgattcatc aggagagcaa cgtaatcgtt cgcgcattcc
    23761 gcgattactt acgccaggac atcggtgaaa tccttatcga taacccgaaa gtgctcgaac
    23821 tggcacgtca gcatatcgct gcattaggcc gcccggattt cagcagcaaa atcaaactgt
    23881 acaccggcga gatcccgctg ttcagccact accagatcga gtcgcagatc gagtccgcct
    23941 tccagcgtga agttcgtctg ccatccggtg gttcgattgt tatcgacagc accgaagcgt
    24001 taacggccat cgacatcaac tccgcacgcg cgacccgcgg cggcgatatc gaagaaaccg
    24061 cgtttaacac taacctcgaa gctgccgatg agattgctcg tcagctgcgc ctgcgtgacc
    24121 tcggcggcct gattgttatc gacttcatcg acatgacgcc tgtacgccac cagcgtgcgg
    24181 tagaaaaccg tctgcgtgaa gcggtgcgtc aggaccgtgc gcgtattcaa atcagccata
    24241 tttctcgctt tggcctgctg gaaatgtccc gtcagcgcct gagcccatca ctgggtgaat
    24301 ccagccatca cgtctgcccg cgctgctccg gtaccggtac cgtgcgtgac aacgaatcgc
    24361 tgtcgctctc tattctgcgt ctgatcgaag aagaagcgct gaaagagaac acccaggaag
    24421 ttcacgccat tgttcctgtg ccaatcgctt cttacctgct gaatgaaaaa cgttctgcgg
    24481 taaatgcgat tgaaactcgt caggacggcg tacgctgtgt gattgtgcca aacgatcaga
    24541 tggaaacccc gcactaccat gtgctgcgcg tgcgcaaagg ggaagaaacc tcaaccttaa
    24601 gctacatgct gccgaagctg catgaagaag cgatggcgct gccgtctgaa gaagagttcg
    24661 ctgaacgtaa gcgtccggaa caacctgcgc tggcaacctt tgccatgccg gatgtgccgc
    24721 cagctccaac cccagctgaa cctgccgcgc ctgttgtagc cccagcacct aaagctgcaa
    24781 cggcaacacc agcatctcct gcacaaccag ggttgttgag ccgcttcttc ggcgcactga
    24841 aagcgctgtt cagcggtggt gaagaaacca aaccggccga gcaatcagca ccgaaagcag
    24901 aagcgaaacc ggaacgtcaa caggatcgtc gcaagcctcg tcagaacaac cgccgtgacc
    24961 gtaatgagcg ccgcgatacc cgtagtgaac gtacagaagg cagcgataat cgcgaagaaa
    25021 accgtcgtaa tcgtcgccag gcacagcagc agactgccga gacgcgtgag agccgtcagc
    25081 aggcagaggt aacggaaaaa gcgcgtacca cagacgagca gcaagcgccg cgtcgtgaac
    25141 gtagccgccg ccgtaacgat gataaacgtc aggcgcaaca agaagtgaag gcgctgaatg
    25201 ttgaagagca atctgttcag gaaaccgaac aggaagaacg tgtacgtccg gttcagccac
    25261 gtcgtaaaca gcgtcagctc aatcagaaag tgcgttacga gcaaagcgta gccgaagaag
    25321 cggtagtcgc accggtggtt gaagaaactg tcgcagccga accaattgtt caggaagcgg
    25381 cagccccacg cacagaactg gtgaaagttc cgctgccagt cgtagcgcaa actgcaccag
    25441 aacagcaaga agagaacaat gctgataacc gtgacaacgg tggcatgccg cgtcgttctc
    25501 gccgctcgcc tcgtcacctg cgcgtaagtg gtcagcgtcg tcgtcgctat cgtgacgagc
    25561 gttatccaac ccagtcgcca atgccgttga ccgtagcgtg cgcgtctccg gaactggcct
    25621 ctggcaaagt ctggatccgc tatccaattg tacgtccgca agatgtacag gttgaagagc
    25681 agcgcgaaca ggaagaagta caagtgcagc cgatggtgac tgaggtccct gtcgccgccg
    25741 ctgccgaacc tgttgttagc gcgccagttg ttgaagaagt ggctgaagtc gtagaagccc
    25801 ccgttcaggt tgccgaaccg caaccggaag tggttgaaac gacgcatcct gaagtgattg
    25861 ctgccgcggt aactgaacag ccgcaggtga ttaccgaatc tgatgttgcc gtagcccagg
    25921 aagttgcaga acacgcagaa ccggtggttg aaccgcagga agagacggca gatattgaag
    25981 aagttgccga aactgctgag gttgtggttg ctgaacctga agttgttgct caacctgccg
    26041 cgccagtcgt cgctgaagtc acagcagaag ttgaaacggt agctgcggtt gaacctgagg
    26101 tcaccgttga gcataaccac gctaccgcgc cgatgacgcg cgctccggca ccggaatatg
    26161 ttccggaagc accgcgtcac agtgactggc agcgccctac ttttaccttc gaaggcaaag
    26221 gtgccgcagg tggtcatacg gcaacgcatc atgcctccgc cgctcctgcg cgtccgcaac
    26281 ctgttgagta ataattagct caagcaatca agccctggta actgccaggg cttttttatt
    26341 tcatctttga gtcaacatat taaaattcat caaatggcat ccttgctaac caacaataca
    26401 aaataggcaa aaaaatgccc gccccggcga tcccagagcg ggcagacaaa acatacccag
    26461 tttcatgata tgttttaaag cgagcttatt tgttgagctg gaagagcgac aatccctgca
    26521 tatcggtaaa tgctttatac gatgcctgca atgccgtttg ctgcatgatg taagatgaaa
    26581 tagttgcatt ccagtccaca tcaaccagat cgctcatctg ctgcgtttgc cctaaagcgc
    26641 gatcgctacc taatgaatcc agcgactcca gttcgttcag ttgcgtgcct aattccgcgc
    26701 gaacagtcag cacattgttc agcgagtttt tcagtccgcg gttggtttta tccagtgccg
    26761 cagcggcggt ttctttatcc gcttcgctat ccgcgaccgg cgttttcagg gctgcgatgg
    26821 cgctatccag catggcaaaa agattggttt cagaagcgct accgtctggt tccgctaccg
    26881 cgttgctggt aatactgtcg aaaattttgt cgcccgtatg tcctatcacc atcgaacgcg
    26941 aagcatcgac ctgttgttta atactttctg ttccgccgac ataatcaccg tcagcctcac
    27001 taaacggcgc agtctctgtt ttataaccgg caaaaatgta gcgcccgtta ccgtcagtgg
    27061 tgttcgccag attcagtaac tggtcacgaa gcccctgaat atccgtagcc agcgaggccc
    27121 ggtcatcgtc actcaaggtg ccattgctgg cgtagacaat tttttcctga gcattctgga
    27181 tagcagtggt gacctggcta agtacactct cttccagtga cactttttga gtggcgaaag
    27241 tacgcgccag cgtgtactgg ctgttttgcg cctgtgcctg ggagagaaca acagcttgtg
    27301 atgcagcaat gggatcatct gaagggttaa cgactcgctt acccgtcgac atctgttcgc
    27361 cgtacttcat ccattctgcc tgagaattgg tgatgccacg catgttttgc tggtacatca
    27421 tctgggtact gaaacgcatt ttatctcccc ttagcgaatg ttaatcagcg catcaaaaat
    27481 cgcgtttgcc gtctgcagaa cctgcgcatt cgccaggtaa tactgctgaa aacgttgcag
    27541 atttccgtac tcctcatcga gattgacacc ggaaatcgac tgctgctgat tggaaagctg
    27601 cgtcaccaca ttaccttgcg tggcgctact ggttttcaac gtcgcggttt tattcccgat
    27661 atcactcact aatgaggcat aagcgtcgtt aaaggatttc gcaccgccca ccgttttact
    27721 gttgctttgc agatccagca gggcctgacc gttgcggttg tcgctatcac ccgcatcttc
    27781 ttcgctcgcc atggcgattt tcgcttcgtc agtgattaat acatccatgt tgacgatggc
    27841 gtcacttacc ggtttcagcg tgaagctgtc gttaacggca ggcgttcctg taaacgtcaa
    27901 ctccagacca tcaaatgcca ctttaccgtt ggcatccggc gtcaccgtaa aagtggtatt
    27961 gctggcaagg cgggtgacct gccactgatt attatcgaac gagattttgt aatctgtcgc
    28021 cagtaccgcg gaggcatcag ttaccgtggc accgatcgca acgtcaccgt tgtttttagt
    28081 gttttgcaga accgcgggct taccgatagc aaagaaatct ttaccggcat cgccgttggc
    28141 atcaaatccg gctttgtgtt gggtgttgaa agcctcggca aatgccagcg ccagttgtcc
    28201 gagcgtatta cgcgtctggt ccagatcctg agaacggaat gtcagaatgc cgcccagcga
    28261 cccggtattc agtaatttct ccgggatctc aatattgcca gccgtcccat caatataagc
    28321 gacagttgta cgcgaagggt cggcactgga aggaactgcc gccagctgcc gcgccgtact
    28381 tccctgaacc agtgagtagc cattgaccat cgtgatgtta taagtgccac catcctgaac
    28441 gctaacttct acgccaacaa tctggtttaa ttcactcacc agttgatcgc gttgatccag
    28501 cagattgtta ggtgacgccc ctgcccccac gcctgtcaga cgcgagattt gatcgttcag
    28561 gctggcgatt tgtttagcgt aattgttgat ctgatcaacg ctggcaccta tcgcgatatt
    28621 gacctgtttg tcctggtcgc gcagatattg atcggtggtt ttaaactgat tcaccaaccc
    28681 ttctgatttc ccaatcagcg cctggcgcgc tgccgggtct tccgcgttac ttaccagcgt
    28741 ttgcaggctt gtgaagaaat cctgcatctg tgttgccagc gaagaggtac tggtggagag
    28801 catattgtcg attttcgaca tctgctcata gcgggcagtc aggccgctac tttgcgtctg
    28861 cgccgcacgt aactggttgg taataaatgc atcatactca cgctgcacac cagaaacgta
    28921 gacgccattg ccaacccagc cgccagcgcc caacgtgcta ttggcctgcg ccataatagt
    28981 ggtttggcgg gtatatccgg caacgttata gctggagata ttattacttg ccgtatttaa
    29041 cgccacctgg gccgcgttca gtccgctcat ggcgttattg atcaagctgg acatggaggt
    29101 tccttataag ccttcaatta cagagtatta tcggcagcga cccgccggac ttgagttatt
    29161 cagaacagat tatcaatgtt catgctgtag gttttgctca ccttgtcgct tatcgatttc
    29221 atctgctgaa tcatgctggt gagtttgcgg gcatagtgag gatcggtggc atagcccgcg
    29281 tcctgtaggg cctgcgcccc ctgctccgca ctcgcggcgg tcgtcacggc ggcgtagcgc
    29341 gggttacgcg ttaacagccc aacgtaatcc gacaatgctt ccagatacga gctgtagacc
    29401 cgaaacttcg cttttacttt cttcgcttcg ccattttcat attcagtcgt ggtgatttca
    29461 gtgactggcc ctttccagtt gccagaggct ttgacgccaa acaggttata gctcggctcg
    29521 ccgttttcac ggcggatttg ccgttgtccc cagccagatt ccagcgccgc ctgagcgagg
    29581 atcaaatgat gtggcacacc gctttgctgg cttgccagtt gggcgggcaa cgagagttgc
    29641 gcgaggaatg ctttactgtc acccggcagc gaatcatcgt agttacgtgg tacggccttt
    29701 tgcaccagct gcgaaagcgt ctgattttga taacgcacca cggtttcgag cgggaatttc
    29761 atcggtgctg ctggcgtggg ctcctctggc aatggttgtt ctggcgtcat ctgtttaacc
    29821 atcatctctg caagccccag acctttgccc gccgtcatct gttgggcaat ctgctggtca
    29881 tacatactgg tatacaggcg agtgtgctcg ctgctgaaca ggccatcttt tggtaaagcg
    29941 tcgcgcatgc ttttcaacat catctgcacg aacatccctt ccacctggcg ggccaccgga
    30001 cggatatttg ccgctggatc ttcgccagct ttcgccttta gttcgttgag tgattgcgca
    30061 tcccaggccg cacttgccag tagtttgctg tcgctgatca tcagatgatt tccagttttg
    30121 cccgcagaca tcccgcactt tgcattgatt gcaaaataga catcagatcc atcggcgtag
    30181 cgcccagcgc attgagcgcg cgcaccacgt tattgaggct ggcgctggaa cgtacgcttt
    30241 gcagcgaacc gccgctctgg cgtaaatcga tctgcgtttg tggagtaacc acagtctgtc
    30301 caccaccaaa cggtgtatct ggctggctga cattggcctg acgattgacc gtaaccgaga
    30361 gattcccctg cgctactgcg cagctgtcga gggtcacttc gcgattcatc accaccgagc
    30421 cagtgcgcga gttaatcact actttagcgt cctgcggggt gacattaacc tgcatattct
    30481 gaatatcggc aaggaaacgg acctgggaac tgttgccact tggtacgcgc acctgaatag
    30541 tccgcgcatc taacgcggtg gcgctgccat atccacgcac gcggttgatg gtgtcagcga
    30601 tttgctgcgc catactgaaa tcttcgtcgt taagttgcaa attaagggca ttcccgacgc
    30661 caaactggct gggcaattca cgttcaataa ccgcaccatt ggtgatccgt ccaccgttca
    30721 gttggttcac ctgaacactg ctaccgccag cggaggcccc tgcgccgcca accagaatat
    30781 tgccctgtgc cagcgcatac acctgactgt caacgccctt aagcggtgtc atcaataacg
    30841 taccgccacg cagacttttg gcatttccca tggaagaaac caccacgtca atggtttgcc
    30901 cctggcgtcc aaacggtgga agtgacgccg tcaccattac cgcagcgacg ttttttagct
    30961 gcatattagt gcccgtcgga acggtaattc ccagctgtga gagcatgtta ttaagcgttt
    31021 gtgtggtaaa cggcgtctga gttgtctggt caccggtgcc atccagcccc accaccaggc
    31081 catagccaat cagtgagttt tgccttaccc cctgaacact ggtgagatcg cgaatacgct
    31141 cagcctgagc cgccgtcgta accagtagaa gaattaatgc agagagaaat ttaatcacca
    31201 cagcctcact tacattggcg acaggttaag gaagaaacgc tgcaaccagc ccatattttg
    31261 cgcttcgtta atgtagccat tgcctacgta ttcaatgcgc gcatccgcca cctgagtaga
    31321 tggtacggta ttgctgccgc tgatagtgcg tggattaacc acgccagaga agcgaataaa
    31381 ttcggtaccc tgattaatag cgatctgttt ttcacccacc acatgcaggt tgccgttgac
    31441 cagtacctgg tcaaccgtca ccgtcaacgt accgctaaag gtattgctgg cattggcccc
    31501 gccttttccg ttgaacgtgt taccaccgga ggcttcgaca tcggcacgag cgttaccaaa
    31561 caacccctgc aaatagcgcg gcacagtatc aaagccaaaa ttagttttac cgtcacggct
    31621 ggcattcgca gaggagcttt tgctggcgct gacgttctcc tgcaacacga tagtcagcgt
    31681 atcgccaata ttgcgtggtc gacgatcttc aaacagcggt tgatagccat agttaatcgg
    31741 ctgagcagac tggaaaatag acccgttggc gacgggcgtc ggaccgggaa ccggttgtgc
    31801 actggtcgcc ccctgtacca gcggcgtgga gggtatccag gcgcagccgg ttagtgaaag
    31861 caccaacaag ctggaaatgg cataagtatg cgcagcgttt ttttgcatgg ctatcatctt
    31921 caaaaatcag taaaccggtg aacctgccac cggttaagcc ttagagttgc gtcagttttt
    31981 gcagcatctg atcggtggtg gacaccgctt tactgttgat ttcgtaagcg cgttgcacct
    32041 gaatcatatt gaccagttct tccgccacgt tgacgttaga cgtttcaaca tacccttgat
    32101 acagcagtcc cgcgccgttc aggcccggcg tgctttcgtt cggtgcaccg gaggattgcg
    32161 tttcggtgta gaggttttcg ccaatgctct ccagtccggt gtcattcata aaggtggtga
    32221 gattgagctg cccaacctga accggagctg cctggccttg ttgggttacg ctgaccacgc
    32281 catcacgacc gatggtgata cttaacgcat tcgccggaat ggtgatcgct ggctgcacct
    32341 gaaaaccacc ggccgtcacc agctgcccgt tctgatccac ctggaaagag ccgtcacggg
    32401 tataggctga tgagccatct ggcaacatca cctggaaaaa gccctgccct ttaatcgcga
    32461 cgtctttact gttattggtt tgcgacaggt tgccctggct gtgtaaacgt tccgtggcga
    32521 ccgggcgtac ccccgtaccg atttgtaatc cggatggtaa ggtggtttgt tcggaagact
    32581 gtgcccccgg ctggcgaatg gtttgataaa gcagatcttc aaacaccgcg cgctgacgct
    32641 taaaaccgtt ggtactaacg tttgccaggt tgttggcaat gacgtccata ttggtttgct
    32701 gggcgtcaag gcccgttttg gcgatccata atgaactgat cataaaatgt cctgtagtgg
    32761 attagtgaca aagtgcatat gtcttcttgc cggatgcggc gtaaacgcct tatccggcct
    32821 acaagagctc gcaaaatcga taaattgcga ttgcccccat agacccgata aacttgcgca
    32881 tagggcaact tatacttgtc atgtatcctt tcaattaact catcgacagc agttggttgg
    32941 cacggcctgc gttatcatcg acgctgctga tcaccttcat ctgcatttca aaacgccgtg
    33001 cgctggcaat catgtcgctc attgccgcaa cggcattgac gttactgcct tccagaaccc
    33061 ccgacatcac acgcaaggtt ggatctgcct gcagtaccgg cccacgccta gcctggcttt
    33121 ctgcgcttaa acgaaaaatg ccatcgtcac cgcgctggac ttcgctgccc gtggctttca
    33181 ccagcttcag acgccccact ggcgcaaccg tatttgccgg atcacccaga ttcagcgccg
    33241 agattgtgcc atcggcagca atagtgattt ccgccccttc cggcacagca attggccccg
    33301 cctcgcctac caccggatgt ccctgaattg tcagttgccc ggtgggatca acctgaatgc
    33361 tgccattacg cgtataccct tcactgccgt cagcggtctg cacgaccagc caaccatcct
    33421 gctgcaacgc aacgtccagc gggcgcgagg tgtaatccat cttgccgggc gtcatgtctg
    33481 cacccggtgt tgacgccgtt accagcgtgc gcgtgggcag tgaaagccct tccaccggca
    33541 ctgcgcgcaa ggcgtttaac tgcgcgcgaa aaccgggcgt tgaggcattg gccagattac
    33601 tggcggttac cgcctgttga ttcagcgtct ggctggctgc tcccatcgcg gtataaattg
    33661 cgtgatccat tgagctatcc cgtcagcgat tagcgtaagt taaccagcgt attgaggatc
    33721 tggtcctggg ttttgatggt ctgggcgtta gactgatagt tacgctgggc aacgatcata
    33781 ttgaccagtt ctttactgag atcgacgttg gacgcttcca gcgcaccgtt ggtcagtgtg
    33841 ccaaagttgc ccgtcccggc tgtccctaac agcgccacgc cagaagattg cgtagcggac
    33901 cagacgttgt cgccttcgga tgccagacct tcgttgttgg caaagttcgc cagtacaatc
    33961 tgccccagca gttgggtttg ttcgttggaa tagttgccga caaccgtacc gtcatcattg
    34021 atttgataac tcaccagatc gcccggtttg tagccgttct gggtggttgc cacaatgttg
    34081 ttagcgccgg tattttgctg catggagttg aggaagctca ggctaaacgt ggcgggatct
    34141 gcgccgttaa ttgcgccagt ggaaatattc gctgttggat ttgaggtcag aacgccattg
    34201 gcattaaaca ccagtgtcgt tgcagggtta gctgtgcctg ttggatcact gctatccagg
    34261 gtgtagacct gccagttatt atccccggtc ttcacaaagt agacgctcat gtcatgagca
    34321 ttaccctgac tatcgaaaac agtcaccgaa ccttttttgt tatagctatc cgcattgctg
    34381 gcatcaaatg cgttaacaga gggaagcgaa tcactggaat tcaggttgat ctgcatcgac
    34441 gccgtggtgg tagttttcgc tgccatcagg gtattcggaa tcgaaatatt ggtcggattc
    34501 gccccttgct gaatagtcgg cggcgtaccg gttgccgggt aacccgtcag ctgtaaacct
    34561 tgcatattca ccaggttacg gttttcatcc agcttaaatt gtccgttacg gctgtagaac
    34621 accgaaccat tgctgtctac cagacggaaa aaaccgttct ggctaatagc aacgtccaga
    34681 ccacgcccgg tgttggtggt cgtgccatcg gtaaagtcct gagtgatacc agcaactttt
    34741 acccccagtc ccactttcga accggcaaac atatcggcaa aagaggccgt gcctgattta
    34801 aagccgtagg tggcggagtt ggcgatattg ttgccaataa catcgaggtt ggtggcggca
    34861 gcgtttaatc cgctaaccgc ttgagaaaag gccatgactg actcctgaaa tgtgaaggct
    34921 taaattatct gccgtacttc gtcgagggta gtggtgccgt aagtgccgag atccagcgta
    34981 ttaccgttgt tgccgcggat caccccctgc accagagcaa actgcagcgg ttgggcaacc
    35041 agttgtgtac caccgttact ggcgctaatc gctacattgt aagaaccgtt cagcgcagtg
    35101 ctgccgtcag tcaacgtacc gtcccaggtg aaactatgga ctccggcagt cagttcaccg
    35161 atatcaatgg tacggacgac cgcgccgttt ttatcggtga tggtggccgt cactttgtct
    35221 gctgcctgtt gcaactcaac accaaacggc gtggtcgtgg tcacagcccc ttcttcactg
    35281 ccggttcccg caagaacagt ggtaccgggg atcatcacgc catgaccgat aaggttactg
    35341 gcctgtaacg actggctgtt atcaatctgt ccggaaatag atccgagcgt ggtattgagt
    35401 ttttcaatcc cactgaccgt gctgatttgt gccaattgcg acgtcagctc gttgttttcc
    35461 attggattgg tcgggtcctg gtttttcagc tgcgccacca acaaagtcag aaaactgctt
    35521 tgtaaatctg cggcgttgct gcccgtgagc gaactactgc tggtggtact gacgccggta
    35581 ttcgtcggat cggtggtggt taccgcaata gacatagctt tctcctttat tgaccgagcg
    35641 taagggtttt cagcatcatg cttttcaccg tgttgagcac ttcaacattg gcctgataac
    35701 tgcgtgacgc cgacatggtg ttaaccatct ctccgacgac atcaacgttt ggcattttca
    35761 cgtagccctt tgcgtccgcc agcggattac ccggttcata aaccagtttg tccggagcct
    35821 gactttctat aacatcggca acctttacgc cgcctgtcgc agcacctggc gcagcgttaa
    35881 cctggaatac cacctgtttt gcccgatatg gctgtccatc gggaccggtg acgctatcag
    35941 cattcgccag attactggcc gccacgttca ggcgctggga ctgggcagtt aacgccgacc
    36001 cggcgatatc aaaaatattc agcagtgcca tccgttaatt tccgctctgt aaaacgttca
    36061 tcatgccttt gatctgcccg ctcaacgcgc taaggctcat ctggtattgc aggctgttat
    36121 cggcaaactg ggtgcgttcg cgatccatat cgacagtatt accgtcaagc gaaggctggt
    36181 ccggaatacg gtattgcagt tctgcggtag gaggcgtcag cgcctgcgcc ggaatgtgtt
    36241 tcgttgaggt catcgtcaga gcaaccacac tggttgcatc ccgtccacgt tgcatgactt
    36301 ttttaagttc actggcaaaa tcgatatcgc gcgcctgata accaggggta tcggcattgg
    36361 cgatgtttgc tgccagcact tcctgacgct gggcgcgcag attgagcgcc tcttgttgaa
    36421 aacgtaaggc ggcgtcgagc ttatcgagca tatctcctcc gcaggtatca aaattcggcc
    36481 atcagcttaa atgcctttac gcgcgcgtta tcggcggaat aaacgcaaaa tgggtcgcta
    36541 tttatgccgt tgatggtcat tgcggacagg tacaattcac gttgtagaaa tggctgggga
    36601 cgaaaatgct gacaataaaa cgtagcgtgg cgatcatcgc gatactgttc agtccgttaa
    36661 gtacggcgag caatctcaca tcgcaattgc acaacttttt tagcgcccaa ctcgcggggg
    36721 taagtgatga ggttcgtgtt tctattcgta cagcacccaa tctgctaccg ccgtgcgatc
    36781 agccattgct ttcgatgggc aataattccc gtctgtgggg caatgtgaat gtgttggcac
    36841 gctgcggtaa cgacaaacga tatttacagg ttaatgtgca ggccacagga aattatgtgg
    36901 ttgccgcgat gcccattgtg cgtggaggaa agctggaagc tggcaatgtc aaactgaaac
    36961 gcggacggct ggataccttg ccaccgcgta cggtgctgga tatcaatcaa cttgttgatg
    37021 ccgttagcct gcgcgatcta tcacccgatc aacctatcca gttaacccac tttcgccagg
    37081 catggcgggt aaaagcggga caacgtgtca acgtgatcgc cagcggtgat gggtttagcg
    37141 ccaacgcaga aggtcaggcg ctgaacaatg cagccgtcgc acagaatgcg cgggtgcgca
    37201 tgatatcggg acaagtggtc agcggcgttg ttgatgcaga tgggaatatt cttataaacc
    37261 tgtaagctgt taaagattac ccgtcccttg ccgataaata agcaacacat gataaaagcg
    37321 ccctcaatga ggaataaacc atgagtattg atcgcacttc gcctctgaag ccagtaagca
    37381 ccgttcaacc gcgcgaaacc actgacgcgc cggtaacaaa cacccgggcg gcaaaaacaa
    37441 ccgcctccac cagcaccagt gtgacgttaa gcgacgcgca agcaaaactg atgcaacccg
    37501 gcagcagtga tatcaatctt gaacgtgtcg aagcgttaaa gctggcgatt cgtaacggtg
    37561 aactaaaaat ggacaccggc aaaattgccg atgcgctgat caacgaagcg cagcaagact
    37621 tgcagagtaa ttgatcgtat gacgcgtctt gcagagatcc tcgaccagat gtccgctgtg
    37681 cttaacgatc tcaaaacggt aatggatcaa gagcagcaac atctctctat ggggcagatc
    37741 aacggcagcc agttgcaatg gattacagaa caaaaaagct cactgctggc gacgctggat
    37801 tacctcgaac agttacgcag gaaagaaccc aatacagcaa atagcgttga tattagtcaa
    37861 cgctggcagg aaattactgg caaaacgcag caactacgcc aactgaatca acataacggc
    37921 tggttactgg aaggacagat tgaacgcaat caacaggcgc tggaaatgtt gaaaccgcat
    37981 caggaaccga cgctgtatgg ggcgaacggt cagacctcaa caacccatcg cggcagtaaa
    38041 aagatttcga tctgaagatt aactccggcc tgcactgcgg gccggaatgt atcgttacac
    38101 cgtccggcgg gcaaattctt taactttgaa gcccagtacc gccagtgcag cgaaatacgc
    38161 ggcaatcccc gccagcacga ccgccattaa acgcagtaaa cgccagggca tggtacccaa
    38221 tgaccactcc ggcatgatat gtaacatacc taaaagcacg ccagacatca ccagtaccgc
    38281 caccaccaga cgcaacagaa acgccatcca gccgggttgc ggggtaaaga ttttctgctt
    38341 acgcaactgc cagtaaagca gcgaagcatt cagacacgcc gccagaccaa tagaaagtga
    38401 cagcccggca tgtttcaacg gaccaataaa cgccaggttc atcaattgcg ttaaaatcag
    38461 cgtaacgatg gcaattttca ctggcgtttt aatgtcctgg cgggaataaa agccaggagc
    38521 caacactttc actacaatca ggccaatcaa acccaccgag taggcaatta acgcccgctg
    38581 ggtcatcagc gcatcaaagg cggtaaattt accgtactgg aacagcgaaa cggttaacgg
    38641 accggaaaga atgcccaacg caaccgcact cggcagcgcc aacaggaaac aaagacgcag
    38701 cccccagtcc atcaaacggt tgtattcatc atgattgcca ctggcaaaac ttttcgacag
    38761 cgacggtagc aaaatggtgc caagcgccac gcccagcaca ccggacggaa actccattaa
    38821 gcggtcggcg taatacatcc acgacaccga accggaagca agaaacgagg caaaaatggt
    38881 gttgatgatt aaggagatct ggctcacaga gacgccaagg attgccggtc ccatctgttt
    38941 caccacacgc attgctccgg catcgtggaa gttaatgcgt ggcaaaacca gcatgccgat
    39001 cttcttcaga tgaggtagct gatacaccag ctgcaggatg ccgccgaccg taacagccca
    39061 ggccagcgcc agcaccggtg gattaaagta cggcgcggca aacagcgcaa aaccaatcat
    39121 gctgatattg agcaatgtcg gggcaaacgc cggaattgag aagcggttcc aggtattcag
    39181 aatcgctccc accagcgacg ccagggagat cagcaagata tagggaaagg ttatctttag
    39241 tagctggctg gtcagggcaa atttgtcggc agtgtcagcg aagccaggcg cggtcaccat
    39301 gatcacccac ggtgcagcga gcatgccagc gaccgtcaca accgccagcg caagtgtcag
    39361 caggccagaa acataagaga caaagacccg cgtggcgtct tcaccctgct tgcttttata
    39421 ttctgccaga atcggtacaa atgcctggga aaatgcccct tcggcaaaga tacggcgtaa
    39481 caagttagga agtttaaaag cgacgaaaaa ggcgtcggtt gccatccctg cgccaaagat
    39541 tctggcgaca attgcgtctc gtgcgaagcc aagcacacgc gaaaacatgg tcatcgagct
    39601 gacggcggcc agcgatttta ataaattcat cggtgttcta atccagaccc acgggcaaaa
    39661 cgcctgcaag gcaggcgctg aaagaggcga atagtctacc tggattatgg tgaattgcta
    39721 ccgccagatg ttacagggtt tattcactca tcgcatcgcg ccagatcttg tcaacgatac
    39781 gttgcgccag cacggcctgt tcgccggcgg tttgcggaac tgtctgattt tgcacgcatt
    39841 caatgaagtg ccgcgcacag ccgacaaatc cacgttgctc gagcgtactc tgccaactag
    39901 gaatcggttt atgcactacg ccctgcccgc gctcctcacg ccattcgcgc atatccgtaa
    39961 tgtcgatgag cgcaccatca gtcacggcct gcacggtttc acgctgactt ccggcacgac
    40021 gatgcataca ggtggtgatc tgcaaaggac cagccgaaaa atggtgctcg gcaaacagca
    40081 tttcgccagc gtcgttagtc agtaacgtac cgccatccag agaggctttg ccacctgaca
    40141 accacagcgc ggtatccacc acatgcagat aatcatccag caacgtgaaa taaagatcgt
    40201 gtggcccgac gctattggta cgatgtttat ccattcttag cgaggacgcg gtggcgagtt
    40261 gcgtttttaa ctcaccgtag agtggtgcga aacgacggtt aaaaccgacc atcaacgtca
    40321 gttttttccg cgccgccagt tccaccagcc gttcagcatc gcgcagattt tctgccagcg
    40381 gtttatcgac acagacatgt acccccgcat tgagtaacgt actgaccacg tcaaagtggc
    40441 tggcggtgct ggaatgcaca aaaaccgcat cgcaactggc ggcaaggctg gataacgaat
    40501 cagcataagg aatgcgccag ctgtcacaaa ttagcaaggc tttggcgcgc gtaggcgacc
    40561 acgcaccttg taacgtccag tcagacgctg ccgccagcac cggtaaccac gctttttgcg
    40621 cgatgccgcc taaccccact acgccgatac gtaatttttt cactttaatc tcccagatgg
    40681 gccagcaacg aatcaagacg ctgtttcagt tctgccactt cgatttccag ggcttcgacg
    40741 cgggcctgta aatcaccgtc agctgcgttc gacatatccg ttaccgccgg cggattctca
    40801 acctcaccac tgaaaagatg catgtagcgg ctttcgcgtt tacccggttc gcgggccaga
    40861 cgcaccacaa aaggaccatc ttcgcgattc gccagttgtt ccagcgtcga ctccacttcc
    40921 gccatatcgc tgaattcata cattcgcgcg gcgcggctgc gcagttcccc cggcgtctgg
    40981 gcaccacgca ataacaacgt ggtgattaac gccacttctg cggcgctcag tttcagatcg
    41041 ccaaattctg aattacaaaa acgttgctca tatttggtga cccgattacc aaaaccgctc
    41101 accgtgcgta gataatgacg tttgaccaga ttatccagct gttcctgcac ttcagattca
    41161 ctcagattca taaccggttc acggttcgtt ttctgattac aggccgtgac tacaccattt
    41221 acggagagcg ggtattgctc cggcgtggtc acctgttttt ccagcaagca accaatcacg
    41281 cgagcttcca tgacagtaag ttgatatttc atgagattct ccttagcggc cgggcgtcca
    41341 gtctggggta gttaatgccg tcagtacgtg atcgcgccat tgtccatcaa tcaacagata
    41401 gtctttcgca tagccttctt tttcaaaacc cagtcgcgcc agtaaatcac cgctgcgttt
    41461 attgtgcggc atataattag ccataatgcg atgaatatgt tgggtgcgct gcatataacg
    41521 aatggctgcg ctcagagctt caaacatgag tcctttgccc tgccattttt gcccaatcga
    41581 ataaccgaga tagcaggcat gaaaagagcc gcgaacaaca ttggaaaaat tggctacgcc
    41641 aataatctct ttttcgtccg ggtcgaataa gccaaagtaa aaagctgaac cttgtttatg
    41701 aaattcgtta atcatcccta gcctggcctg ccagcctgat ggataacagt ggctttcgtc
    41761 gcgcactggc tcccagggct tgaggaaatg gcgattctct gcgtaataat ccgccagacg
    41821 ccaggcatca cgatcatgca ccagacgcac gaccagtcgg tctgtggtta agcgcacttt
    41881 tggcacgtta ctgcgatagc caaacattct atacctactc cttcccgtaa cgtctttaat
    41941 acgctgtaag tattactata cctgcgcaac cagaccctgt gaaaagtcca gaatgccatt
    42001 ttctgcatta ttcacatatt tgataaaaac tctctatcaa agcgacgttt tgataaaaaa
    42061 atattgtccc cgaagtgatg ggaaaagtct tagcggcaat gcagaataga aagtgctcat
    42121 cttttatttc ccgggagggg aaatgtcccg cgtgtcgcag gcgaggaacc tgggtaaata
    42181 tttcctgctc atcgataata tgctggtcgt gctggggttc tttgttgtct tcccgctgat
    42241 ctctatccgc ttcgttgatc aaatgggctg ggccgccgtc atggtcggta ttgccctcgg
    42301 tctacgccag tttattcagc aaggtctggg tattttcggc ggcgcaattg ccgaccgctt
    42361 tggtgccaaa ccgatgattg tcaccggtat gctgatgcgt gccgccggat tcgccacaat
    42421 gggtatcgcc cacgaaccgt ggctactgtg gttttcatgc ctgctttcgg gactcggcgg
    42481 cacgttgttt gatcctccgc gttcggcgct ggtggtgaaa ttaatccgtc cacagcagcg
    42541 tggtcgtttt ttctcgctgt tgatgatgca ggacagtgcc ggtgcggtca ttggcgcatt
    42601 gttggggagc tggctgttgc aatacgactt tcgcctggtc tgcgccacag gggcagtttt
    42661 gtttgtgctg tgtgcagcgt tcaatgcgtg gttgttaccg gcatggaaac tctccaccgt
    42721 gcgcacgccc gttcgcgaag gcatgacccg cgtgatgcgc gacaagcgtt ttgtcaccta
    42781 tgtcctgacg ctggcgggtt actacatgct ggctgtacaa gtgatgctga tgctgccaat
    42841 tatggtcaac gacgtggctg gcgcgccctc tgccgttaaa tggatgtatg ccattgaagc
    42901 gtgtctgtcg ttaacgttgc tctaccctat cgcccgctgg agtgaaaagc attttcgtct
    42961 ggaacaccgg ttaatggctg ggctgttgat aatgtcatta agcatgatgc cagtgggcat
    43021 ggtcagcggc ctgcaacaac ttttcaccct gatttgtctg ttttatatcg ggtcgatcat
    43081 tgccgagcct gcgcgtgaaa ccttaagtgc ttcgctggca gacgcaagag ctcgcggcag
    43141 ctatatgggg tttagccgtc tgggtctggc gattggcggc gctattggtt atatcggtgg
    43201 cggctggctg tttgatctgg gcaaatcggc gcaccagcca gagcttccgt ggatgatgct
    43261 gggcattatt ggcatcttca ctttccttgc gctgggttgg cagtttagcc agaaacgcgc
    43321 cgcgcgtcgt ttgcttgaac gcgacgcctg atttgatact cccccctgac tcctccatac
    43381 tgaaagtgaa ctgacagcca aatggaggag tcacgtgaag ctatacattt acgatcactg
    43441 cccttactgc ctcaaagccc gcatgatttt cggcctgaaa aatatccccg tcgaattaca
    43501 tgttctgctc aacgacgacg cagaaacacc cacccggatg gtcggtcaaa aacaggttcc
    43561 cattctgcaa aaagatgaca gccgctatat gccagaaagc atggacatcg ttcactatgt
    43621 cgataaactc gacggcaaac cgttactgac cggcaaacgt tcccctgcca ttgaagagtg
    43681 gctgcgcaag gtcaatggct acgccaacaa actgctgttg ccgcgttttg ccaaatcggc
    43741 atttgatgag ttttctactc ctgccgcgcg caaatatttc gtcgacaaga aagaggccag
    43801 cgcgggtaat tttgccgacc tgctggccca ctctgacggt ctgattaaga atatcagcga
    43861 tgatttacgt gcgctggaca aactgatcgt caaaccgaac gccgtgaatg gcgaactttc
    43921 ggaagatgat attcagctat ttccgctcct gcgtaatctg acgctagtag ccggaatcaa
    43981 ctggccaagc cgcgttgctg attaccgcga taatatggcg aaacagacac aaatcaattt
    44041 gttatcatca atggcgattt aatcctgacc cgccgcggca gcctgtattc gcgggttgcc
    44101 gccgttttca gttttttctg ctggcgcgtt tatcgctggt gacacatgct gcgttgtgtc
    44161 aggatattga cgcagatttc atgtcgagga ctaccatgaa caagttttta tttgccgctg
    44221 cattgatcgt cagtggcctg ctcgttggct gtaatcaact cacccaatac accatcaccg
    44281 aacaagaaat taaccagtcg cttgcgaaac ataataattt ctcaaaagat atcggtttac
    44341 ccggcgtggc tgacgcccat attgttctga caaacctgac cagccaaatt ggtcgcgaag
    44401 agccgaataa ggttacccta accggagacg ccaatctgga catgaactcc ctgttcggta
    44461 gccagaaagc gaccatgaaa ctgaagctga aagcgctgcc cgtgtttgat aaagaaaaag
    44521 gcgcgatctt cctgaaagag atggaagtgg tcgatgcgac ggtacaaccg gaaaaaatgc
    44581 aaacggtgat gcaaacgttg cttccctatt taaaccaggc attacgcaat tactataacc
    44641 agcaacctgc ttacgtcctg cgcgaagatg gcagccaggg cgaagcaatg gcgaagaaac
    44701 tggcgaaagg cattgaagtg aagccaggcg aaattgtcat tccgtttact gattaatcac
    44761 gagggcgcat tcgcgccctt tatttttcgt gcaaaggaaa acgtttccgc ttatcctttg
    44821 tgtccggcaa aaacatccct tcagccggag catagagatt aatgactgca ccatcccagg
    44881 tattaaagat ccgccgccca gacgactggc accttcacct ccgcgatggc gacatgttaa
    44941 aaactgtcgt gccgtgtacc agcgaaattt atggacgggc tatcgtaatg cccaatctgg
    45001 ctccgcccgt gaccaccgtt gaggctgccg tggcgtatcg ccagcgcatt cttgacgccg
    45061 tacctgccgg gcacgatttc accccgctga tgacctgtta tttaacagat tcgctggatc
    45121 ctaatgagct ggagcgcgga tttaacgaag gcgtgttcac cgctgcaaaa ctttatccgg
    45181 caaacgcaac cactaactcc agccacggtg ttacgtcagt tgacgcaatc atgccggtac
    45241 ttgagcgcat ggaaaaaatc ggtatgccgc tactggtgca tggtgaagta acacatgcag
    45301 atatcgacat ttttgatcgt gaagcgcgct ttatagaaag cgtgatggaa ccgctgcgcc
    45361 agcgcctgac cgcgctgaaa gtcgtttttg agcatatcac caccaaagac gctgccgact
    45421 atgtccgtga cggaaatgaa cggctggctg ccaccatcac tccgcagcat ctgatgttta
    45481 accgcaacca tatgctggtt ggtggcgtgc gtccgcacct gtattgtcta cccatcctca
    45541 aacgcaatat ccaccaacat gcattgcgtg aactggtcgc cagcggtttt aatcgtgtat
    45601 tcctcggaac ggattctgcg ccacatgcac gtcatcgcaa agagagcagc tgcggctgcg
    45661 cgggctgttt caacgcccca accgcgctgg gcagttacgc taccgtcttt gaagagatga
    45721 atgctttgca gcactttgaa gcattctgtt ctgtaaacgg cccgcagttc tatggcttgc
    45781 cggtcaacga cacattcatc gaactggtac gtgaagagca acaggttgct gaaagcatcg
    45841 cactgactga tgacacgctg gtgccattcc tcgccggaga aacggtacgc tggtccgtta
    45901 aacaataaaa atcgtagctt cctgttgtca ttaggttatt ttacctgtat aaataaccag
    45961 tatattcaac agggggctat tatgcgaatt gaagtcacca tagcgaaaac ttctccattg
    46021 ccagctgggg ctattgacgc cctggctggc gaactttccc gccgtattca gtatgcgttt
    46081 cctgataatg aaggccacgt atcggtacgt tatgccgcag caaataattt atcggttatt
    46141 ggcgcgacaa aagaagataa acagcgcatt agcgaaattc tccaggaaac gtgggaaagc
    46201 gccgatgact ggtttgtcag cgaataatat gcagtgattt ttgtttttgc cgggtcgccc
    46261 cggctttttt attttacttg cgaattgtga gtacaaaatt tctacacttc ctcaaaataa
    46321 ttgcacaata tgctaaaaaa atatgaatga catgctgttt tattgttcat ataaatctta
    46381 aaagcagaa
//
LOCUS       DADSYM010000014        44299 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2415
            SAMN08039579-rid11723173.denovo.014, whole genome shotgun sequence.
ACCESSION   DADSYM010000014 DADSYM010000000
VERSION     DADSYM010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039579
            Sequence Read Archive: SRR6362533
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 44299)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 44299)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980162.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 03:39:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,484
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 4,347
            CDSs (with protein)               :: 4,347
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 43 of 85
            Pseudo Genes (incomplete)         :: 37 of 85
            Pseudo Genes (internal stop)      :: 19 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..44299
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039579-rid11723173.denovo.014"
                     /strain="LGE2415"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8302 N 95.9628 W"
                     /collection_date="2015-08-24"
     gene            268..939
                     /locus_tag="J1Y83_000671"
     CDS             268..939
                     /locus_tag="J1Y83_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016241930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HBA7628855.1"
                     /translation="MDKVLDSAILSSANKRKGILAIGAHPDDIELGCGASLARLAQKG
                     IYIATVVMTTGNSGVDGIIDRHEESRNALKILGCHQTIHLNFADTRAHLQLNDMISAL
                     ENIIKNQIPSDVEIIRVYTMHDADRHQDHLAVYQASMVACRAIPQILGYETPSTWLSF
                     MPQVFESVKEEYFSLKLTALKKHKSQSQRDYMRPERLRAVAQFRGQQVNSDLGEGFVI
                     HKMIL"
     gene            949..2145
                     /locus_tag="J1Y83_000672"
     CDS             949..2145
                     /locus_tag="J1Y83_000672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HBA7628856.1"
                     /translation="MKTWIFICMAVAILLWFLSTLRRKPSQKKGCIDAIIPAYNEGPC
                     LAQSLDNLLRNPYFCRVICVNDGSTDNTEAVMAEVKRKWGDRFVAVTQKNTGKGGALM
                     NGLNYATCDQVFLSDADTYVPPDQDGMGYMLAEIERGADAVGGIPSTALKGASLLPHI
                     RATVKLPMIVMKRTLQQLLGGAPFIISGACGMFRTDVLRKFGFSDRTKVEDLDLTWTL
                     VANGYRIRQANRCIVYPQECNSPREEWRRWRRWIVGYAVCMRLHKRLLFSRFGIFSIF
                     PMLLVVLYGVGIYLTTWFNEFITTGPHGVVLAMFPLIWVGVVCVIGAFSAWFHRCWLL
                     VPLAPLSVVYVLLAYAIWIIYGLIAFFTGREPQRDKPTRYSALVEASTAYSHPSVTGT
                     EKLSEA"
     gene            2123..2704
                     /locus_tag="J1Y83_000673"
     CDS             2123..2704
                     /locus_tag="J1Y83_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012602184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HBA7628857.1"
                     /translation="MKNYLKLNVKEKNIQIADQVIIDESAGEVVIGANTRICHGAVIQ
                     GPVVIGANCLIGNYAFIRPGTIISNGVKIGFATEIKNAVIEAEATIGPQCFIADSVVA
                     NQAYLGAQVRTSNHRLDEQPVSVRTPDGIIATGCDKLGCYIGQRSRLGVQVIILPGRI
                     ISPNTQLGPRVIVERNLPTGTYSLRQELIRTGD"
     gene            2706..3479
                     /gene="yaiO"
                     /locus_tag="J1Y83_000674"
     CDS             2706..3479
                     /gene="yaiO"
                     /locus_tag="J1Y83_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiO family outer membrane beta-barrel protein"
                     /protein_id="HBA7628858.1"
                     /translation="MIKRTLLAAAIFSALPVYAGLTSITAGYDFTDYSGDHGNRNLAY
                     AELVAKVENATLLFNLSQGRRDYETEHFNATRGQGAVWYKWNNWLTTRTGIAFADNTP
                     VFARQDFRQDINLALLPKTLFTTGYRYTKYYDDVEVDAWQGGISLYTGPVITSYRYTH
                     YDSSDAGGSYSNMISVRLNDPRGTGYTQLWLSRGTGAYTYDWTPETRYGSMKSISLQR
                     IQPLTEQLNLGLTAGKVWYDTPTDDYNGLQLAAHLTWKF"
     gene            3668..3943
                     /gene="frmR"
                     /locus_tag="J1Y83_000675"
     CDS             3668..3943
                     /gene="frmR"
                     /locus_tag="J1Y83_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414891.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formaldehyde-responsive transcriptional
                     repressor FrmR"
                     /protein_id="HBA7628859.1"
                     /translation="MPSTPEEKKKVLTRVRRIRGQIDALERSLEGDAECRAILQQIAA
                     VRGAANGLMAEVLESHIRETFDRNDCYSREVSQSVDDTIELVRAYLK"
     gene            3978..5087
                     /locus_tag="J1Y83_000676"
     CDS             3978..5087
                     /locus_tag="J1Y83_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-(hydroxymethyl)glutathione dehydrogenase"
                     /protein_id="HBA7628860.1"
                     /translation="MKSRAAVAFAPGKPLEIVEIDVAPPKKGEVLIKVTHTGVCHTDA
                     FTLSGDDPEGVFPVVLGHEGAGVVVEVGEGVTSVKPGDHVIPLYTAECGECEFCRSGK
                     TNLCVAVRETQGKGLMPDGTTRFSYNGQPLYHYMGCSTFSEYTVVAEVSLAKINPEAN
                     HEHVCLLGCGVTTGIGAVHNTAKVQPGDSVAVFGLGAIGLAVVQGARQAKAGRIIAID
                     TNPKKFELARRFGATDCINPNDYDKPIKDVLLDINKWGIDHTFECIGNVNVMRAALES
                     AHRGWGQSVIIGVAGSGQEISTRPFQLVTGRVWKGSAFGGVKGRSQLPGMVEDAMKGD
                     IDLEPFVTHTMSLDEINDAFDLMHEGKSIRTVIRY"
     gene            5180..6013
                     /locus_tag="J1Y83_000677"
     CDS             5180..6013
                     /locus_tag="J1Y83_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-formylglutathione hydrolase FrmB"
                     /protein_id="HBA7628861.1"
                     /translation="MELIEKHASFGGWQNVYRHYSQSLKCEMNVGVYLPPKAENEKLP
                     VLYWLSGLTCNEQNFITKSGMQRYAAEHNIIVVAPDTSPRGSHVADADRYDLGQGAGF
                     YLNATQAPWNEHYKMYDYIRNELPNLVMHHFPATARKSISGHSMGGLGALVLALRNPD
                     EYASVSAFSPIVSPSQVPWGQQAFAAYLGENKDAWLDYDPVSLISQGQRVAEIMVDQG
                     LSDDFYAEQLRTPNLEKICQEMNIKTLIRYHEGYDHSYYFVSSFIGEHIAYHANKLNM
                     R"
     gene            complement(6240..6779)
                     /locus_tag="J1Y83_000678"
     CDS             complement(6240..6779)
                     /locus_tag="J1Y83_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414888.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2058 domain-containing protein"
                     /protein_id="HBA7628862.1"
                     /translation="MAKLTLQEQLLKAGLVTSKKAAKVERTAKKSRVQAREARAAVEE
                     NKKAQLERDKQLSEQQKQAALAKEYKAQVKQLIEMNRITIANGDIGFNFTDGNLIKKI
                     FVDKLTQTQLINGRLAIARLLVDNNSEGEYAIIPASVADKIAQRDASSIVLHSALSAE
                     EQDEDDPYADFKVPDDLMW"
     gene            6846..6944
                     /locus_tag="J1Y83_000679"
                     /pseudo
     CDS             6846..6944
                     /locus_tag="J1Y83_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308426.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GlxA family transcriptional regulator"
     gene            6981..8063
                     /locus_tag="J1Y83_000680"
     CDS             6981..8063
                     /locus_tag="J1Y83_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414879.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBA7628863.1"
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQACHVSAKTREKVEAAMAE
                     LNYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERS
                     GVEACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACANVPALFLDVSDQTPINSII
                     FSHEDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREG
                     DWSAMSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDT
                     EDSSCYIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLPPNT
                     QTASPRALADSLMQLARQVSRLESGQ"
     gene            8186..11260
                     /locus_tag="J1Y83_000681"
     CDS             8186..11260
                     /locus_tag="J1Y83_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HBA7628864.1"
                     /translation="MTMITDSLAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEAR
                     TDRPSQQLRSLNSEWRFAWFPAPEAVPESWLECDLPDADTVVVPSNWQMHGYDAPIYT
                     NVTYPITVNPPFVPAENPTGCYSLTFNIDESWLQEGQTRIIFDGVNSAFHLWCNGRWV
                     GYGQDSRLPSEFDLSAFLRAGENRLAVMVLRWSDGSYLEDQDMWRMSGIFRDVSLLHK
                     PTTQISDFQVTTLFNDDFSRAVLEAEVQMYGELRDELRVTVSLWQGETQVASGTAPFG
                     GEIIDERGGYADRVTLRLNVENPELWSAEIPNLYRAVVELHTADGTLIEAEACDVGFR
                     EVRIENGLLLLNGKPLLIRGVNRHEHHPLHGQVMDEQTMVQDILLMKQNNFNAVRCSH
                     YPNHPLWYTLCDRYGLYVVDEANIETHGMVPMNRLTDDPRWLSAMSERVTRMVQRDRN
                     HPSVIIWSLGNESGHGANHDALYRWIKSVDPSRPVQYEGGGADTTATDIICPMYARVD
                     EDQPFPAVPKWSIKKWLSLPGEMRPLILCEYAHAMGNSLGGFAKYWQAFRQYPRLQGG
                     FVWDWVDQSLIKYDENGNPWSAYGGDFGDTPNDRQFCMNGLVFADRTPHPALTEAKHQ
                     QQYFQFRLSGRTIEVTSEYLFRHSDNEFLHWMVALDGKPLASGEVPLDVGPQGKQLIE
                     LPELPQPESAGQLWLTVRVVQPNTTAWSEAGHISAWQQWRLAENLSVTLPSASHAIPQ
                     LTTSGTDFCIELGNKRWQFNRQSGFLSQMWIGDEKQLLTPLRDQFTRAPLDNDIGVSE
                     ATRIDPNAWVERWKAAGHYQAEAALLQCTADTLADAVLITTAHAWQHQGKTLFISRKT
                     YRIDGHGEMVINVDVAVASDTPHPARIGLTCQLAQVAERVNWLGLGPQENYPDRLTAA
                     CFDRWDLPLSDMYTPYVFPSENGLRCGTRELNYGPHQWRGDFQFNISRYSQQQLMETS
                     HRHLLHAEEGTWLNIDGFHMGIGGDDSWSPSVSAEFQLSAGRYHYQLVWCQK"
     gene            11312..12565
                     /gene="lacY"
                     /locus_tag="J1Y83_000682"
     CDS             11312..12565
                     /gene="lacY"
                     /locus_tag="J1Y83_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactose permease"
                     /protein_id="HBA7628865.1"
                     /translation="MYYLKNTNFWMFGLFFFFYFFIMGAYFPFFPIWLHDINHISKSD
                     TGIIFAAISLFSLLFQPLFGLLSDKLGLRKYLLWIITGMLVMFAPFFIFIFGPLLQYN
                     ILVGSIVGGIYLGFCFNAGAPAVEAFIEKVSRRSNFEFGRARMFGCVGWALCASIVGI
                     MFTINNQFVFWLGSGCALILAVLLFFAKTDAPSSATVANAVGANHSAFSLKLALELFR
                     QPKLWFLSLYVIGVSCTYDVFDQQFANFFTSFFATGEQGTRVFGYVTTMGELLNASIM
                     FFAPLIINRIGGKNALLLAGTIMSVRIIGSSFATSALEVVILKTLHMFEVPFLLVGCF
                     KYITSQFEVRFSATIYLVCFCFFKQLAMIFMSVLAGNMYESIGFQGAYLVLGLVALGF
                     TLISVFTLSGPGPLSLLRRQVNEVA"
     gene            12631..13242
                     /gene="lacA"
                     /locus_tag="J1Y83_000683"
     CDS             12631..13242
                     /gene="lacA"
                     /locus_tag="J1Y83_000683"
                     /EC_number="2.3.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactoside O-acetyltransferase"
                     /protein_id="HBA7628866.1"
                     /translation="MNMSMRERIKAGKLFTDMCEGLPEKRLRGKTLMYEFNHSHPSEF
                     EKRESLIKEMFATVGENAWVEPPVYFSYGSNIHIGRNFYANFNLTIVDDYTVTIGDNV
                     LIAPNVTLSVTGHPVHHELRKNGEMYSFPITIGNNVWIGSHVVINPGVTIGDNSVIGA
                     GSVVTKDIPPNVVAAGVPCRIIREINDRDKQYYFKDFKVESSV"
     gene            complement(13363..14646)
                     /locus_tag="J1Y83_000684"
     CDS             complement(13363..14646)
                     /locus_tag="J1Y83_000684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytosine deaminase"
                     /protein_id="HBA7628867.1"
                     /translation="MSNNALQTIINARLPGKEGLWQIHLHDGKISAIDTQSGVMPVTE
                     NSLDAEQGLVLPPFVEPHIHLDTTQTAGQPNWNQSGTLFEGIERWAERKALLTHDDVK
                     QRAWQTLKWQIANGIQHVRTHVDVSDATLTALKAMLEVKQEVAPWIDLQIVAFPQEGI
                     LSYPNGEALLEEALRLGADVVGAIPHFEFTREYGVESLHKTFALAQKYDRLIDVHCDE
                     IDDEQSRFVETVAALAHREGMGARVTASHTTAMHSYNGAYTSRLFRLLKMSGINFVAN
                     PLVNIHLQGRFDTYPKRRGITRVKEMLESGINVCFGHDDVFDPWYPLGTANMLQVLHM
                     GLHVCQLMGYGQINDGLNLITHHSARTLNLQDYGIAAGNSANLIILPAENGFDALRRQ
                     VPVRYSVRGGKVIASTQPAQTTVYLEQPEAIDYKR"
     gene            complement(14636..15895)
                     /gene="codB"
                     /locus_tag="J1Y83_000685"
     CDS             complement(14636..15895)
                     /gene="codB"
                     /locus_tag="J1Y83_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytosine permease"
                     /protein_id="HBA7628868.1"
                     /translation="MSQDNNFSQGPVPQSARKGVLALTFVMLGLTFFSASMWTGGTLG
                     TGLSYHDFFLAVLIGNLLLGIYTSFLGYIGAKTGLTTHLLARFSFGVKGSWLPSLLLG
                     GTQVGWFGVGVAMFSIPVGKATGLDINLLIAVSGLLMTVTVFFGISALTVLSVIAVPA
                     IACLGGYSVWLAVNGMGGLDVLKAVVPAQPLDFNVALALVVGSFISAGTLTADFVRFG
                     RNAKLAVLVAMVAFFLGNSLMFIFGAAGAAALGMADISDVMIAQGLLLPAIVVLGLNI
                     WTTNDNALYASGLGFANITGMSSKTLSVINGIIGTVCALWLYNNFVGWLTFLSAAIPP
                     VGGVIIADYLMNRRRYEHFATTRMMSVNWVAILAVALGIAAGHWLPGIVPVNAVLGGA
                     LSYLILNPILNRKTTAAMTHVEANSVE"
     gene            complement(16220..18106)
                     /gene="prpE"
                     /locus_tag="J1Y83_000686"
     CDS             complement(16220..18106)
                     /gene="prpE"
                     /locus_tag="J1Y83_000686"
                     /EC_number="6.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propionate--CoA ligase"
                     /protein_id="HBA7628869.1"
                     /translation="MSFSEFYQRSINEPEQFWAEQARRIDWQTPFTQTLDHSNPPFAR
                     WFCEGRTNLCHNAIDRWLEKQPEALALIAVSSETEEERTFTFRQLHDEVNAVASMLRS
                     LGVQRGDRVLVYMPMIAEAHITLLACARIGAIHSVVFGGFASHSVAARIDDAKPVLIV
                     SADAGARGGKIIPYKKLLDDAISQAQHQPRHVLLVDRGLAKMARVSGRDVDFASLRHQ
                     HIGARVPVAWLESNETSCILYTSGTTGKPKGVQRDVGGYAVALATSMDTIFGGKAGGV
                     FFCASDIGWVVGHSYIVYAPLLAGMATIVYEGLPTWPDCGVWWKIVEKYQVSRMFSAP
                     TAIRVLKKFPTAEIRKHDLSSLEVLYLAGEPLDEPTASWVSNTLDVPVIDNYWQTESG
                     WPIMAIARGLDDRPTRLGSPGVPMYGYNVQLLNEVTGEPCGVNEKGMLVVEGPLPPGC
                     IQTIWGDDDRFVKTYWSLFSRPVYATFDWGIRDADGYHFILGRTDDVINVAGHRLGTR
                     EIEESISSHPGVAEVAVVGVKDALKGQVAVAFVIPKESDSLEDREVAHLQEKAIMALV
                     DSQIGNFGRPAHVWFVSQLPKTRSGKMLRRTIQAICEGRDPGDLTTIDDPASLDQIRQ
                     AMEE"
     gene            complement(18146..19597)
                     /gene="prpD"
                     /locus_tag="J1Y83_000687"
     CDS             complement(18146..19597)
                     /gene="prpD"
                     /locus_tag="J1Y83_000687"
                     /EC_number="4.2.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-methylcitrate dehydratase"
                     /protein_id="HBA7628870.1"
                     /translation="MSAQINNIRPEFDREIVDIVDYVMNYEISSRVAYDTAHYCLLDT
                     LGCGLEALEYPACKKLLGPIVPGTVVPNGVRVPGTQFQLDPVQAAFNIGAMIRWLDFN
                     DTWLAAEWGHPSDNLGGILATADWLSRNAVASGKAPLTMKQVLTGMIKAHEIQGCIAL
                     ENSFNRVGLDHVLLVKVASTAVVAEMLGLTREEILNAVSLAWVDGQSLRTYRHAPNTG
                     TRKSWAAGDATSRAVRLALMAKTGEMGYPSALTAPVWGFYDVSFKGESFRFQRPYGSY
                     VMENVLFKISFPAEFHSQTAVEAAMTLYEQMQAAGKTAADIEKVTIRTHEACIRIIDK
                     KGPLNNPADRDHCIQYMVAIPLLFGRLTAADYEDNVAQDKRIDALREKINCFEDPAFT
                     ADYHDPEKRAIANAITLEFTDGTRFEEVVVEYPIGHARRRQDGIPKLVDKFKINIARQ
                     FPTRQQQRILEVSLDRTRLEQMPVNEYLDLYVI"
     gene            complement(19631..20800)
                     /gene="prpC"
                     /locus_tag="J1Y83_000688"
     CDS             complement(19631..20800)
                     /gene="prpC"
                     /locus_tag="J1Y83_000688"
                     /EC_number="2.3.3.16"
                     /EC_number="2.3.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-methylcitrate synthase"
                     /protein_id="HBA7628871.1"
                     /translation="MSDTTILQNSTHVIKPKKSVALSGVPAGNTALCTVGKSGNDLHY
                     RGYDILDLAEHCEFEEVAHLLIHGKLPTRDELAAYKTKLKALRGLPANVRTVLEALPA
                     ASHPMDVMRTGVSALGCTLPEKEGHTVSGARDIADKLLASLSSILLYWYHYSHNGERI
                     QPETDDDSIGGHFLHLLHGEKPSQSWEKAMHISLVLYAEHEFNASTFTSRVIAGTGSD
                     MYSAIIGAIGALRGPKHGGANEVSLEIQQRYETPDEAEADIRKRVENKEVVIGFGHPV
                     YTIADPRHQVIKRVAKQLSQEGGSLKMYNIADRLETVMWESKKMFPNLDWFSAVSYNM
                     MGVPTEMFTPLFVIARVTGWAAHIIEQRQDNKIIRPSANYVGPEDRQFVALDKRQ"
     gene            complement(20960..21850)
                     /gene="prpB"
                     /locus_tag="J1Y83_000689"
     CDS             complement(20960..21850)
                     /gene="prpB"
                     /locus_tag="J1Y83_000689"
                     /EC_number="4.1.3.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylisocitrate lyase"
                     /protein_id="HBA7628872.1"
                     /translation="MSLHSPGKAFRAALTKENPLQIVGTINANHALLAQRAGYQAIYL
                     SGGGVAAGSLGLPDLGISTLDDVLTDIRRITDVCSLPLLVDADIGFGSSAFNVARTVK
                     SMIKAGAAGLHIEDQVGAKRCGHRPNKAIVSKEEMVDRIRAAVDAKTDPDFVIMARTD
                     ALAVEGLDAAIERAEAYVEAGAEMLFPEAITELTMYRQFADAVKVPILANITEFGATP
                     LFTTDELRSAHVAMALYPLSAFRAMNRAAEHVYNILRQEGTQKSVIDTMQTRNELYES
                     INYYQYEEKLDDLFARGQVK"
     gene            22089..23675
                     /gene="prpR"
                     /locus_tag="J1Y83_000690"
     CDS             22089..23675
                     /gene="prpR"
                     /locus_tag="J1Y83_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propionate catabolism operon regulatory protein
                     PrpR"
                     /protein_id="HBA7628873.1"
                     /translation="MAHPPRLNDDKPVIWTVSVTRLFELFRDISLEFDHLANITPIQL
                     GFEKAVTYIRKKLANERCDAIIAAGSNGAYLKSRLSVPVILIKPSGYDVLQALAKAGK
                     LTSSIGVVTYQETIPALVAFQKTFNLRLDQRSYITEEDARGQINELKANGTEAVVGAG
                     LITDLAEEAGMTGIFIYSAATVRQAFSDALDMTRMSLRHNTHDATRNALRTRYVLGDM
                     LGQSPQMEQVRQTILLYARSSAAVLIEGETGTGKELAAQAIHREYFSRHDARQGKKSH
                     PFVAVNCGAIAESLLEAELFGYEEGAFTGSRRGGRAGLFEIAHGGTLFLDEIGEMPLP
                     LQTRLLRVLEEKEVTRVGGHQPVPVDVRVISATHCNLEEDMQQGQFRRDLFYRLSILR
                     LQLPPLRERVADILPLAESFLKMSLAALSVPFSAALRQGLETCQILLLLYDWPGNIRE
                     LRNMMERLALFLSVEPTPDLTPQFLQLLLPELARKSAKTPIPGLLTAQQALEKFNGDK
                     TAAANYLGISRTTFWRRLKS"
     gene            complement(23776..24051)
                     /locus_tag="J1Y83_000691"
     CDS             complement(23776..24051)
                     /locus_tag="J1Y83_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HBA7628874.1"
                     /translation="MKIISKMLVGALAFAVTNVYAAELMTKAEFEKVASQYEKIGDIS
                     TSNEMSTADAKEDLIKKADEKGADVLVLTSGQTDNKIHGTANIYKKK"
     gene            24198..24884
                     /locus_tag="J1Y83_000692"
     CDS             24198..24884
                     /locus_tag="J1Y83_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family transporter"
                     /protein_id="HBA7628875.1"
                     /translation="MMKLLHLFMDEITMDPLHAVYLTVGLFVITFFNPGANLFVVVQT
                     SLASGRRAGVLTGLGVALGDAFYSGLGLFGLATLITQCEEIFSLIRIVGGAYLLWFAW
                     CSSMRRHSTPQMSTLQQPISAPWYVFFRRGLITDLSNPQTVLFFISIFSVTLHAETPT
                     WARLMAWAGIVLASIIWRVFLSQAFSLPAVRRAYGRMQRVASRVIGAIIGAIIGVFAL
                     RLIYEGVTQR"
     gene            complement(24901..25144)
                     /locus_tag="J1Y83_000693"
                     /pseudo
     CDS             complement(24901..25144)
                     /locus_tag="J1Y83_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414861.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(25766..26815)
                     /gene="yahK"
                     /locus_tag="J1Y83_000694"
     CDS             complement(25766..26815)
                     /gene="yahK"
                     /locus_tag="J1Y83_000694"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent aldehyde reductase YahK"
                     /protein_id="HBA7628876.1"
                     /translation="MKIKAVGAYSAKQPLEPMDITRREPGPHDVKIEIAYCGVCHSDL
                     HQVRSEWAGTVYPCVPGHEIVGRVVAVGDQVEKHAPGDLVGVGCIVDSCKHCEECEDG
                     LENYCDYMTGTYNSPTPDEPGHTLGGYSQQIVVHERYVLRIRHPQEQLAAVAPLLCAG
                     ITTYSPLRHWQAGPGKKVGVVGIGGLGHMGIKLAHAMGAHVVAFTTSESKREAAKALG
                     ADEVVNSRNADEMVAHVKSFDFILNTVAAPHNLDDFTTLLKRDGTITLVGAPATPHKS
                     PEVFNLIMKRRAIAGSMIGGIPETQEMLDFCAEHGIVADIEMIRADQINEAYERMLRG
                     DVKYRFVIDNRTLTD"
     gene            complement(27192..28574)
                     /locus_tag="J1Y83_000695"
     CDS             complement(27192..28574)
                     /locus_tag="J1Y83_000695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HBA7628877.1"
                     /translation="MKENNSRREFLSQSGKMVTAAALFGPSVPLAHAGGAGTLNCEAN
                     NTMKITDPHYYLDNVLLETGFDYENGVAVQTRTARQTVEIQNGKIVALRENKQHPDAT
                     LPHYDAGGKLMLPTTRDMHIHLDKTFYGGPWRSLNRPAGTTIQDMIKLEQKMLPELQP
                     YTQERAEKLIDLLQSKGTTIARSHCNIEPVSGLKNLQNLQAVLARRQAGFKCEIVAFP
                     QHGLLLSKSESLMREAMQAGAHYVGGLDPTSVDGAMEKSLDTMFQIALDYDKGVDIHL
                     HETTPAGVAAINYMVETVEKTPQLKGKLTISHAFALATLNEQQVDELAHRMAAQRISI
                     ASTVPIGTLHMPLKQLHDKGVKVMTGTDSVIDHWSPYGLGDMLEKANLYAQLYIRPNE
                     QNLSRSLFLATGDILPLNEKGERVWPKAQDDASFVLVDASCSAEAVARISPRTATFHK
                     GQLVWGSVAG"
     gene            complement(28584..29534)
                     /locus_tag="J1Y83_000696"
     CDS             complement(28584..29534)
                     /locus_tag="J1Y83_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase family protein"
                     /protein_id="HBA7628878.1"
                     /translation="MKELVVVAIGGNSIIKDNASQSIEHQAEAVKAVADTVLEMLASD
                     YDIVLTHGNGPQVGLDLRRAEIAHEREGLPLTPLANCVADTQGGIGYLIQQALNNRLA
                     RHGEKKAVTVVTQVEVDKNDPGFAHPTKPIGEFFSESQRDELQKANPDWRFVEDAGRG
                     YRRVVASPEPKRIVEAPAIKALIQQGFVVIGAGGGGIPVVRTEAGDYQSVDAVIDKDL
                     STALLAREIHADILVITTGVEKVCIHFGKPQQQALDRVDIATMTRYMQEGHFPPGSML
                     PKIIASLTFLEQGGKEVIITTPECLPAALRGETGTHIIKT"
     gene            complement(29678..31096)
                     /locus_tag="J1Y83_000697"
     CDS             complement(29678..31096)
                     /locus_tag="J1Y83_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1116 domain-containing protein"
                     /protein_id="HBA7628879.1"
                     /translation="MSQSLFSQPLNVINVGIAMFSDDLKKQHVEVTQLDWTPPGQGNM
                     QVVQALDNIADSPLADKITAANQQALERIIQSHPVLIGFDQAINVVPGMTPKTILHAG
                     PPVTWEKMCGAMKGAVTGALVFEGLAKDLDEAAELAASGEITFSPCHEHDCVGSMAGV
                     TSASMFMHIVKNKTYGNIAYTNMSEQMAKILRMGANDQSVIDRLNWMRDVQGPMLRDA
                     MKIIGEIDLRLMLAQALHMGDECHNRNNAGTTLLIQALTPGIIQAGYSVEQQREVFEF
                     VASSDYFSGPTWMAMCKAAMDAAHGIEYSTVVTTMARNGVEFGLRVSGLPGQWFTGPA
                     QQVIGPMFAGYKPEDSGLDIGDSAITETYGIGGFAMATAPAIVALVGGTVEEAIDFSR
                     QMREITLGENPNVTIPLLGFMGVPSAIDITRVGSSGILPVINTAIAHKDAGVGMIGAG
                     IVHPPFACFEKAIFGWCERYGV"
     gene            complement(31096..32643)
                     /gene="fdrA"
                     /locus_tag="J1Y83_000698"
     CDS             complement(31096..32643)
                     /gene="fdrA"
                     /locus_tag="J1Y83_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase FdrA"
                     /protein_id="HBA7628880.1"
                     /translation="MSVKIVIKPNTYFDSVSLMSISTRANKLDGVEQAFVAMATEMNK
                     GVLKNLGLLTPELEQAKNGDLMIVINGKSGVDNEQLLAEIEELFNTKAQSGSHEARYA
                     TIASAKKHIPESNLAVISVNGLFAAREARQALQNDLNVMLFSDNVSVEDELALKQLAH
                     EKGLLMMGPDCGTAIINGAALCFGNAVRRGNIGIVGASGTGSQELSVRIHEFGGGVSQ
                     LIGTGGRDLSEKIGGLMMLDAIGMLENDPQTEIIVLISKPPAPAVARKVLERARACRK
                     PVVACFLGRGETPVDEQGLQFARGSKEAALKAVMLSGVKQEHLDLHTLNQPLIADVRA
                     RLQPQQKYIRGLFCGGTLCDETLFAVMEKHGDVYSNIQPDPEFRLQDINRSIKHTFLD
                     FGDDDFTNGKPHPMIDPTNRISRLIEEARDPEVAVIVMDFVLGFGSHEDPVGSTIEAI
                     KEAKAIAAAEGRELIILAYVLGTDLDTPSLEQQSQMLLDAGVILASSSTNTGLLAREF
                     ICKGEEA"
     gene            complement(32633..33496)
                     /locus_tag="J1Y83_000699"
     CDS             complement(32633..33496)
                     /locus_tag="J1Y83_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2877 domain-containing protein"
                     /protein_id="HBA7628881.1"
                     /translation="MWALTADADFLAQRGQGQVEQVFARAVNIVLPARQQLLTLLCEE
                     YDNAPNSCRLALTHFNGLFRHGDKVQFDDQGITIGQHHHIEMSHCQRWLSPTLQMTAL
                     NFHLIAWQQWHDIIHQHLGENETLFNYRGDNPFYQALNKELHIKRRAVIQAVNDKQNI
                     AVAVASMMGLGIGLTPSADDYLTGLVLILFISGHPAEKYKEEFYLGLQRGRNNTTLLS
                     AITLEAALQQRCRENIHRFIHNIIYDIPGNATQAIEKIKHIGSSSGCDMLYGMADGCA
                     LSQTYGGNYVS"
     gene            complement(33536..34141)
                     /locus_tag="J1Y83_000700"
     CDS             complement(33536..34141)
                     /locus_tag="J1Y83_000700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HBA7628882.1"
                     /translation="MTIKNPPADYLLAAQQGDIDKVKTCLALGVYINTCDRHGKTAIT
                     LASLYQQYACVQALIDAGADINKQDHTCLNPFLISCLNDDLTLLRIILPAKPDLNCVT
                     RFGGVGLTPACEKGHLSIVKELLAHTEINVNQTNHVGWTPLLEAIVLNDGGIKQQAIV
                     QLLLEHGASPHLTDKYGKTPLELARERGFEEIAQLLIAAGA"
     gene            34399..34896
                     /locus_tag="J1Y83_000701"
     CDS             34399..34896
                     /locus_tag="J1Y83_000701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1097 domain-containing protein"
                     /protein_id="HBA7628883.1"
                     /translation="MNGLTATGVTVGICAGLWQLVSSHVGLSQGWELLGTIGFVAFCS
                     FYAAGGGKSGFIRSLAVNYSGMVWAFFAALAAGWLAPVSGISGVWASVITTVPFSAVV
                     VWQGRFWLLSFIPGGFLGMTLFFASGMNWTVTLLGFLAGNCVGIISEYGGQKLSEATT
                     KRDGY"
     gene            34988..35920
                     /locus_tag="J1Y83_000702"
     CDS             34988..35920
                     /locus_tag="J1Y83_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBA7628884.1"
                     /translation="MNSIFTEENLLAFTTAARFGSFSKAAEELGLTTSAISYTIKRME
                     TGLDVVLFTRSTRSIELTESGRYFFRKATDLQNDFHAIKRSIDTISQGIEARVRICIN
                     QLLYTPKHTARLLQVLKKQFPTCQITVTTEVYNGVWDAIINNQANIAIGAPDTLLDGG
                     GIDYTEIGAIRWAFAIAPDHPLAFVPEPIAESQLRLYPNIMVEDTAHTINKKVGWLLH
                     GQESILVPDFNTKCQCQILGEGIGFLPDYMVREAMAQSLLVTRQIHNPRQDSRMLLAT
                     QHSATGQVTQWIKKQFAPNGILTGIYQDLLHREN"
     gene            complement(35962..37050)
                     /locus_tag="J1Y83_000703"
     CDS             complement(35962..37050)
                     /locus_tag="J1Y83_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HBA7628885.1"
                     /translation="MNSCDFRVFLQEFGTTVHLSLPGSVSEKERLLLKLLMQGMSVTE
                     ISQYRNRSAKTISHQKKQLFEKLGIQSDITFWRDIFFQYNPEIISATGNNSHKYINDN
                     HYHHIVTPEAISLALENHEFKPWIQPVFCAQTGVLTGCEVLVRWEHPQTGMIPPDQFI
                     PLAESSGLIVIMTRQLMKQTADILMPVKHLLPDNFHIGINVSAGCFLAAGFEKECLNL
                     VKKLGNDKIKLVLELTERNPIPVTPEARAIFDSLHQHNITFALDDFGTGYATYRYLQA
                     FPVDFIKIDKSFVQMASVDEISGHIVDNIVELARKPGLSIVAEGVETQEQADLMIGKG
                     VHFLQGYLYSPPVPGNKFISEWVMKAGG"
     gene            complement(37928..39961)
                     /gene="betT"
                     /locus_tag="J1Y83_000704"
     CDS             complement(37928..39961)
                     /gene="betT"
                     /locus_tag="J1Y83_000704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline BCCT transporter BetT"
                     /protein_id="HBA7628886.1"
                     /translation="MTDLSHSREKDKINPVVFYTSAGLILLFSLTTILFRDFSALWIG
                     RTLDWVSKTFGWYYLLAATLYIVFVVCIACSRFGSVKLGPEQSKPEFSLLSWAAMLFA
                     AGIGIDLMFFSVAEPVTQYMQPPEGAGQTIEAARQAMVWTLFHYGLTGWSMYALMGMA
                     LGYFSYRYNLPLTIRSALYPIFGKRINGPIGHSVDIAAVIGTIFGIATTLGIGVVQLN
                     YGLSVLFDIPDSMAAKAALIALSVIIATISVTSGVDKGIRVLSELNVALALGLILFVL
                     FMGDTSFLLNALVLNVGDYVNRFMGMTLNSFAFDRPVEWMNNWTLFFWAWWVAWSPFV
                     GLFLARISRGRTIRQFVLGTLIIPFTFTLLWLSVFGNSALYEIIHGGAAFAEEAMVHP
                     ERGFYSLLAQYPAFTFSASVATITGLLFYVTSADSGALVLGNFTSQLKDINSDAPGWL
                     RVFWSVAIGLLTLGMLMTNGISALQNTTVIMGLPFSFVIFFVMAGLYKSLKVEDYRRE
                     SANRDTAPRPLGLQDRLSWKKRLSRLMNYPGTRYTKQMMETVCYPAMEEVAQELRLRG
                     AYVELKNLPPEEGQQLGHLDLLVHMGEEQNFVYQIWPQQYSVPGFTYRARSGKSTYYR
                     LETFLLEGSQGNDLMDYSKEQVITDILDQYERHLNFIHLHREAPGHSVMFPDS"
     gene            40090..40677
                     /gene="betI"
                     /locus_tag="J1Y83_000705"
     CDS             40090..40677
                     /gene="betI"
                     /locus_tag="J1Y83_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308386.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator BetI"
                     /protein_id="HBA7628887.1"
                     /translation="MPKLGMQSIRRRQLIDATLEAINEVGMHDATIAQIARRAGVSTG
                     IISHYFRDKNGLLEATMRDITSQLRDAVLNRLHALPQGSAERRLQAIVGGNFDETQVS
                     SAAMKAWLAFWASSMHQPMLYRLQQVSSRRLLSNLVSEFRRELPRQQAQEAGYGLAAL
                     IDGLWLRAALSGKALDKPLAHSLTRHFITQHLPTD"
     gene            40691..42163
                     /gene="betB"
                     /locus_tag="J1Y83_000706"
     CDS             40691..42163
                     /gene="betB"
                     /locus_tag="J1Y83_000706"
                     /EC_number="1.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="betaine-aldehyde dehydrogenase"
                     /protein_id="HBA7628888.1"
                     /translation="MSRMAEQQLYIHGGYTSATSGRTFETINPANGNVLATVQAAGRE
                     DVDRAVKSAQQGQKIWAAMTAMERSRILRRAVDILRERNDELAKLETLDTGKAYSETS
                     TVDIVTGADVLEYYAGLIPALEGCQIPLRETSFVYTRREPLGVVAGIGAWNYPIQIAL
                     WKSAPALAAGNAMIFKPSEVTPLTALKLAEIYSEAGLPDGVFNVLPGVGAETGQYLTD
                     HPGIAKVSFTGGVASGKKVMANSAASSLKEVTMELGGKSPLIVFDDADLDLAADIAMM
                     ANFFSSGQVCTNGTRVFVPTKCKAAFEQKVLARVERIRAGDVFDPQTNFGPLVSFPHR
                     DNVLRYIAKGIEEGARVLCGGDVLKGDSFDNGAWVAPTVFTDCSDDMTIVREEIFGPV
                     MSILTYESEDEVIRRANDTDYGLAAGIVTADLNRAHRVIHQLEAGICWINTWGESPAE
                     MPVGGYKHSGIGRENGVMTLQSYTQVKSIQVEMAKFQSIF"
     gene            42177..43865
                     /gene="betA"
                     /locus_tag="J1Y83_000707"
     CDS             42177..43865
                     /gene="betA"
                     /locus_tag="J1Y83_000707"
                     /EC_number="1.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline dehydrogenase"
                     /protein_id="HBA7628889.1"
                     /translation="MQFDYIIIGAGSAGNVLATRLTEDPNTTVLLLEAGGPDYRFDFR
                     TQMPAALAFPLQGKRYNWAYETEPEPFMNNRRMECGRGKGLGGSSLINGMCYIRGNAL
                     DLDNWAQEPGLENWSYLDCLPYYRKAETRDVGENDYHGGDGPVSVTTSKPGVNPLFEA
                     MIEAGVQAGYPRTDDLNGYQQEGFGPMDRTVTPQGRRASTARGYLDQAKSRPNLTIRT
                     HAMTDHIFFDGKRAVGVEWLEGDSTIPTRAAANKEVLLCAGAIASPQILQRSGVGNAG
                     LLAEFDIPLVHELPGVGENLQDHLEMYLQYECKEPVSLYPALQWWNQPKIGAEWLFGG
                     TGVGASNHFEAGGFIRSREEFAWPNIQYHFLPVAINYNGSNAVKEHGFQCHVGSMRSP
                     SRGHVRIKSRDPHQHPAILFNYMSHEQDWQEFRDAIRITREIMHQPALDQYRGREISP
                     GVECQTDEQLDEFVRNHAETAFHPCGTCKMGYDEMAVVDAEGRVHGLEGLRVVDASIM
                     PQIITGNLNATTIMIGEKMADMIRGKDALPRSLAAYFVANGMPVRAKKLNRDLN"
BASE COUNT        10508 a        11702 c        11316 g        10773 t
ORIGIN      
        1 tttttaatat gcaattaatt gacaaactaa atcacattta aatacaatat ataaaacaaa
       61 ataaccatac aaaccaaaca cacaaacact taacatatga actacataaa agattacaca
      121 ctcttatccc cgtacctgac agccccttgt ttttcagcta tcagctattt ttaacactta
      181 aaaattagca tctgtctgcg tcagaggtta cgtctctggg ggcgtcgttg tgcttgtagt
      241 ataataaata aaatggagtg tcataaagtg gataaggttt tagattcagc gatcctttct
      301 tcggcaaata aaagaaaggg tatccttgcc attggcgcgc atcctgacga tatagaatta
      361 ggctgtggcg catcgctagc tcgccttgcg caaaaaggaa tttatattgc taccgtggtg
      421 atgactaccg gtaattctgg cgtagatgga ataatcgatc gccacgaaga atcacgcaac
      481 gctctaaaga tattagggtg ccaccaaact attcatctta attttgctga cacccgcgct
      541 catttacagc tcaatgatat gatttctgcc ctggaaaaca tcataaaaaa tcaaattccc
      601 tctgatgttg aaattatacg tgtatatacc atgcatgatg ctgaccgcca tcaggatcat
      661 cttgcagtct atcaagcctc aatggttgcc tgtcgcgcta ttccgcaaat tctcggctat
      721 gaaacaccaa gtacctggct ttcatttatg cctcaggttt ttgaatccgt gaaagaagaa
      781 tatttttctc ttaaactgac tgcgttaaaa aaacataaaa gtcagagcca gcgagattat
      841 atgcgcccgg agcgtctgcg tgcagttgct caatttcgcg ggcaacaggt caatagcgat
      901 ctgggtgaag gttttgttat tcataaaatg attctttgag caaattgcat gaaaacctgg
      961 atctttatat gtatggccgt agcaatattg ctatggtttc tgagtacgtt aaggcgtaag
     1021 cccagccaaa agaaaggctg tattgacgcc attatacctg cttataacga aggcccgtgt
     1081 ctggcgcagt cactggataa tctgctgcgt aacccttatt tttgccgggt aatttgcgtt
     1141 aacgacggct ccacggacaa taccgaagcg gtcatggcgg aagtcaaacg caaatggggc
     1201 gaccgctttg ttgccgtcac gcaaaaaaat accggtaaag gtggtgcgct gatgaatggc
     1261 ctcaattacg ccacctgcga tcaggttttt ttaagtgatg ccgacaccta tgttccgccc
     1321 gatcaagacg gaatgggcta tatgctggcg gaaattgagc gtggcgctga tgccgtaggc
     1381 ggcattccct ctactgcgtt gaaaggcgcg agtctgttac cacacatccg cgcgaccgta
     1441 aagttgccga tgattgttat gaagcgcacg ctacagcagc ttctgggcgg cgcaccgttt
     1501 atcatcagcg gtgcctgcgg gatgttccgt actgatgtat tgcgtaagtt cggtttctcg
     1561 gatcgtacta aagtcgaaga ccttgatctc acctggacgt tggtggcaaa cggctaccgt
     1621 attcggcagg cgaatcgctg catcgtatac ccacaggaat gtaacagtcc gcgtgaggag
     1681 tggcgccgct ggcggcgttg gattgtgggc tacgcggtct gtatgcgcct gcataaaaga
     1741 cttttattta gccgcttcgg tatcttcagt atatttccta tgctgttggt tgtgctttat
     1801 ggcgttggta tttatctcac tacctggttt aatgaattca tcaccaccgg gccacatggt
     1861 gtggtgttgg caatgttccc gcttatctgg gtcggcgtag tttgtgttat tggtgcattt
     1921 agcgcctggt ttcatcgttg ctggttgttg gtgcctttag caccgctttc cgttgtgtat
     1981 gtattattag cttatgccat ctggattatt tatggactta ttgccttttt tactggacgc
     2041 gaacctcagc gcgacaaacc cacccgctat tccgcactgg tggaagcgtc aaccgcttat
     2101 tcccaccctt ctgtcacagg aactgaaaaa ctatctgaag cttaatgtta aagagaaaaa
     2161 tattcagatt gcagaccagg tgattattga tgaaagtgcc ggtgaggttg ttatcggcgc
     2221 gaatacccgt atttgccacg gtgccgttat tcagggtccg gtagtgattg gcgcaaactg
     2281 cctgataggt aattatgcgt ttattcgtcc tggcacaata atcagcaatg gcgtaaaaat
     2341 tggttttgcc accgaaatta aaaatgcggt tattgaagcg gaagccacga ttggtccgca
     2401 atgctttatt gccgactcgg tagttgcaaa ccaggcatat ttgggcgcac aagtacgaac
     2461 cagtaatcat cgtctggatg aacagcccgt gtctgttcga actccagatg gaattatcgc
     2521 taccggatgc gataaattag gttgttatat cgggcagcgt tcacgccttg gcgtacaagt
     2581 tattattttg cctgggcgaa ttatttcccc gaacacgcag cttggcccgc gcgtgattgt
     2641 agaacgtaat ttacctaccg gaacttattc actccgacaa gaacttatcc gtacaggaga
     2701 ttaaaatgat aaaacggacg ttattagcgg cggcaatttt tagcgcatta cccgtttatg
     2761 ccgggttaac ttccattacc gcgggctacg attttaccga ttattctggc gatcatggca
     2821 accgtaattt agcgtatgct gaactggtgg cgaaagttga aaacgcaacg ctgcttttta
     2881 atctttccca ggggcgtcgt gattatgaaa ctgaacattt caatgccact cgcggccagg
     2941 gtgcagtctg gtacaaatgg aataactggc tgacaacccg aacgggtatt gcctttgcgg
     3001 ataatacgcc ggtctttgcc cgccaggatt ttcgtcagga tattaacctg gccctgttgc
     3061 caaaaacgct tttcacgacc ggttatcgct acactaaata ttacgatgat gtcgaagtcg
     3121 atgcctggca aggcggcata tcactctata ctggcccggt gatcaccagc tatcgctata
     3181 cccattatga ctccagcgat gcaggtggta gttacagcaa tatgatttcc gtgcgtctga
     3241 atgacccgcg cggcactggt tacacgcaac tatggctaag ccgcggaaca ggtgcttaca
     3301 cctacgactg gacgccagaa acacgctacg gcagcatgaa gagcatcagt ttacaacgta
     3361 ttcaaccgct gactgagcaa cttaatctcg ggttgacggc aggtaaagtg tggtacgaca
     3421 ccccaaccga tgattataac ggtctgcaac ttgcagccca tctgacctgg aaattctgat
     3481 tccttctgcc gcccgctatc cggggcggcc ttccctgccg attagccccc accccctttc
     3541 ctctttgttt tccgactaca ttcaccggat aaattttatt ctccagtgtt atatactata
     3601 ggggggtatg cattgacata tagcataccc ccctatagta tattgcgtgc agatgatgag
     3661 gtgcgaaatg cccagtactc cggaagagaa gaaaaaggtc cttactcgag ttcgtcgtat
     3721 tcgggggcag attgatgctc tggaacggtc gctggagggt gatgccgaat gccgtgccat
     3781 actccaacag atcgctgccg ttcggggcgc ggctaacggg ctgatggcag aagtgcttga
     3841 aagccatatc cgggaaacgt ttgaccgaaa tgactgctac agccgcgaag tcagccaatc
     3901 cgttgacgac actattgaac tggttcgtgc ctatcttaaa tagctgaatc tattaccata
     3961 ttgaggaaga gcgagagatg aaatcacgtg ctgccgttgc atttgctccc ggtaaaccgc
     4021 tggaaatcgt tgaaattgac gttgcaccac cgaaaaaagg tgaagtgctg attaaagtca
     4081 cccataccgg tgtttgccat accgacgcat ttaccctctc cggcgatgac ccggaaggtg
     4141 tcttcccggt ggttctcggt cacgaagggg ccggggttgt agttgaagtc ggtgaagggg
     4201 taaccagcgt caaacctggc gaccatgtga tcccgcttta caccgcagag tgcggcgagt
     4261 gtgagttctg tcgttctggc aaaactaacc tgtgtgtcgc ggttcgcgaa acgcagggca
     4321 aaggcttgat gccagacggc accacccgtt tttcttacaa cgggcagccg ctttatcact
     4381 acatgggatg ctcaacattc agtgaataca ccgtggtagc ggaagtgtct ctggccaaaa
     4441 ttaatccaga agcaaaccat gaacacgtct gcctgctggg ctgtggcgtg accaccggta
     4501 ttggcgcggt acacaacaca gctaaagtcc agccaggtga ttctgttgcc gtgtttggtc
     4561 ttggcgcgat tggtctggca gtggttcagg gcgcgcgtca ggcgaaagcg ggacggatta
     4621 tcgctatcga taccaacccg aagaaattcg aactggcgcg tcgcttcggt gccaccgact
     4681 gcattaaccc gaatgactac gacaaaccga ttaaagatgt tctgctggat atcaacaaat
     4741 ggggtatcga ccacaccttt gaatgtatcg gtaatgtcaa cgtgatgcgt gcagcgctgg
     4801 aaagcgcgca ccgcggctgg ggtcagtcgg tgatcatcgg ggtagcaggc tccggtcagg
     4861 aaatctccac ccgtccattc cagttggtca ccggtcgcgt atggaaaggt tccgcgtttg
     4921 gcggcgtgaa aggtcgttcc cagttaccgg gtatggttga agatgcgatg aaaggtgata
     4981 tcgatctgga accgtttgtc acgcatacca tgagcctgga tgaaattaat gacgccttcg
     5041 acctgatgca tgaaggtaaa tccattcgaa ccgtaattcg ttactgattt cccgctggtt
     5101 taccccgtcc agttcagatg gggttcttaa tactctcccc gggcagccgt ccgggggatt
     5161 aaccgtgaga taatgactga tggaactcat tgaaaaacat gccagctttg gcggctggca
     5221 aaatgtgtat cggcattatt cccaatcact gaaatgtgaa atgaatgtcg gcgtctatct
     5281 cccaccaaaa gccgagaatg aaaaattgcc ggtgttgtac tggctttcag gcctgacctg
     5341 caacgagcaa aatttcatta ctaaatcggg gatgcagcgt tacgcggctg agcacaacat
     5401 tattgttgtt gcgccggaca ccagtccgcg aggcagtcat gtcgcagatg ctgaccgtta
     5461 cgatctcggg caaggagccg ggttttacct gaacgcgacg caagcgccgt ggaatgaaca
     5521 ttacaaaatg tatgactata tccgcaacga gctgccgaat ttagtgatgc atcattttcc
     5581 agcaacggcc agaaagtcta tctcaggtca ttctatgggc gggctgggcg cgctggtgct
     5641 ggcgttacgt aacccagatg aatatgccag cgtctcggcg ttttcgccca ttgtctcccc
     5701 atcgcaagta ccgtggggac agcaagcctt tgccgcatat cttggtgaaa ataaagatgc
     5761 ctggttggat tacgacccgg tgagtcttat ttcacaaggt caacgcgttg cggaaatcat
     5821 ggttgatcag ggattgagtg atgattttta cgcagaacaa ctgcggactc caaatcttga
     5881 aaagatctgc caggagatga atatcaagac gttaatccgt tatcacgagg gttatgatca
     5941 cagctattat tttgtctcca gttttattgg cgagcatatt gcgtaccacg ccaataaact
     6001 gaatatgcgt tgagcatccg cgcacgcatg ccggatgcgg cgtaaacgcc ttatccggcc
     6061 tacatttcac ccgtaagccg taggccgata agacgcgaaa agcgtcgcat caggcatctg
     6121 cgcacggctg ccggatgcgg cgtgaacgcc ttatccggcc tacacttcgc ccataaaccg
     6181 taggcatgat aagactcgca aagcgtcgca tcaggcatct gcacacgatt tatctatgtt
     6241 taccacatca aatcatcagg cactttgaag tcggcatacg gatcgtcttc atcctgctct
     6301 tcggcgctga gcgcgctgtg caacacaata ctgctggcat cacgctgagc aattttatcg
     6361 gcaacgctgg cgggaataat ggcgtattca ccttcgctat tgttatcgac caacaagcga
     6421 gcaatcgcca gacgaccatt aatcaactgc gtttgcgtga gcttatcaac aaaaatcttc
     6481 ttaatcagat tgccgtcagt gaagttaaaa ccaatatcgc cattggcaat ggtaattcgg
     6541 ttcatttcaa tgagctgctt cacctgggct ttatattctt tcgccaacgc cgcttgtttt
     6601 tgctgctcgc taagctgttt atcacgctca agctgtgcct ttttattttc ttctaccgcc
     6661 gcccgagctt cacgcgcctg aacacgcgat tttttcgctg ttctctcgac cttcgccgct
     6721 tttttgctgg tgactagtcc cgctttgagc aactgctctt gtaaggtaag ttttgccatc
     6781 tttgtttcca aacccactga ataattttta gcgagtatac ctgtaaacgc cgtaggtgta
     6841 ttggcgtggg ttttctctca cttgtgacct ggcgtcagca ttttaaatct cacttcgacg
     6901 tcagccccgc cgaatggcgc aaaacctttc gtggtatggc atgatagcgc ccggaagaga
     6961 gtcaattcag ggtggtgaat gtgaaaccag taacgctata cgatgtcgca gagtatgccg
     7021 gtgtctctta tcagaccgtt tcccgcgtgg tgaaccaggc ctgccacgtt tctgcgaaaa
     7081 cgcgggaaaa agtggaagcg gcgatggcgg agctgaatta cattcccaac cgcgtggcac
     7141 aacaactggc gggcaaacag tcgttgctga ttggcgttgc cacttccagt ctggccctgc
     7201 acgcgccgtc gcaaattgtc gcggcgatta aatctcgcgc cgatcaattg ggtgccagcg
     7261 tggtggtgtc gatggttgaa cgaagcggcg tcgaagcctg taaagcggcg gtgcacaatc
     7321 ttctcgcgca acgcgtcagt ggactgatca ttaactatcc gctggatgac caggatgcca
     7381 ttgctgtgga agcagcctgc gctaatgttc cggcgttatt tcttgatgtc tctgaccaga
     7441 ctcccatcaa cagtattatt ttctcccatg aagacggtac gcgactgggc gtggagcatc
     7501 tggtcgcatt gggtcaccag caaatcgcgc tgttagcagg tccattaagt tctgtctcgg
     7561 cacgtctgcg cctggcgggc tggcataaat atctcactcg caatcaaatt cagccgatag
     7621 cggaacggga aggcgactgg agtgccatgt ccggttttca acaaaccatg caaatgctaa
     7681 atgagggcat cgttcccact gcgatgctgg ttgccaacga tcagatggcg ctgggcgcaa
     7741 tgcgcgccat taccgagtcc gggctgcgcg ttggtgcgga tatctcggta gtgggatacg
     7801 acgataccga agacagctcg tgttatatcc cgccgttaac caccatcaaa caggattttc
     7861 gcctgctggg gcaaaccagc gtggaccgct tgctgcaact ctctcagggc caggcggtga
     7921 agggcaatca gctgttgccc gtctcactgg tgaaaagaaa aaccaccctt ccgcccaata
     7981 cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca cgacaagttt
     8041 cccgactgga aagcgggcag tgagcgcaac acaattaatg tgagtcagct cactcattag
     8101 gcaccccagt ctttacactc tatgtgtccg gctcgtatgt tgtgtgaaat tgtgagcgga
     8161 taacaatttc acacaggata cagctatgac tatgattacg gattctctgg ccgtcgtatt
     8221 acaacgtcgt gactgggaaa accctggcgt tacccaactt aatcgccttg cggcacatcc
     8281 ccctttcgcc agctggcgta atagcgaaga ggcccgcacc gatcgccctt cccaacagtt
     8341 gcgtagcctg aatagcgaat ggcgctttgc ctggtttccg gcaccagaag cggtgccgga
     8401 aagctggctg gagtgcgatc ttcctgacgc cgatactgtc gtcgtcccct caaactggca
     8461 gatgcacggt tacgacgcgc ccatctacac caacgtgacc tatcccatta cggtcaatcc
     8521 gccgtttgtt cccgcggaga atccgacagg ttgttactcg ctcacattta atattgatga
     8581 aagctggcta caggaaggcc agacgcgaat tatttttgat ggcgttaact cggcgtttca
     8641 tctgtggtgc aacgggcgct gggtcggtta cggccaggac agccgtttgc cgtctgaatt
     8701 tgacctgagc gcatttttac gcgccggaga aaaccgcctc gcggtgatgg tgctgcgctg
     8761 gagtgacggc agttatctgg aagatcagga tatgtggcgg atgagcggca ttttccgtga
     8821 cgtctcgttg ctgcataaac cgaccacgca aatcagcgat ttccaagtta ccactctctt
     8881 taatgatgat ttcagccgcg cggtactgga ggcagaagtt cagatgtacg gcgagctgcg
     8941 cgatgaactg cgggtgacgg tttctttgtg gcagggtgaa acgcaggtcg ccagcggcac
     9001 cgcgcctttc ggcggtgaaa ttatcgatga gcgtggcggt tatgccgatc gcgtcacact
     9061 acgcctgaac gttgaaaatc cggaactgtg gagcgccgaa atcccgaatc tctatcgtgc
     9121 agtggttgaa ctgcacaccg ccgacggcac gctgattgaa gcagaagcct gcgacgtcgg
     9181 tttccgcgag gtgcggattg aaaatggtct gctgctgctg aacggcaagc cgttgctgat
     9241 tcgcggcgtt aaccgtcacg agcatcatcc tctgcatggt caggtcatgg atgagcagac
     9301 gatggtgcag gatatcctgc tgatgaagca gaacaacttt aacgccgtgc gctgttcgca
     9361 ttatccgaac catccgctgt ggtacacgct gtgcgaccgc tacggcctgt atgtggtgga
     9421 tgaagccaat attgaaaccc acggcatggt gccaatgaat cgtctgaccg atgatccgcg
     9481 ctggctatcc gcgatgagcg aacgcgtaac gcggatggtg cagcgcgatc gtaatcaccc
     9541 gagtgtgatc atctggtcgc tggggaatga atcaggccac ggcgctaatc acgacgcgct
     9601 gtatcgctgg atcaaatctg tcgatccttc ccgcccggta cagtatgaag gcggcggagc
     9661 cgacaccacg gccaccgata ttatttgccc gatgtacgcg cgcgtggatg aagaccagcc
     9721 cttcccggcg gtgccgaaat ggtccatcaa aaaatggctt tcgctgcctg gagaaatgcg
     9781 cccgctgatc ctttgcgaat atgcccacgc gatgggtaac agtcttggcg gcttcgctaa
     9841 atactggcag gcgtttcgtc agtacccccg tttacagggc ggcttcgtct gggactgggt
     9901 ggatcagtcg ctgattaaat atgatgaaaa cggcaacccg tggtcggctt acggcggtga
     9961 ttttggcgat acgccgaacg atcgccagtt ctgtatgaac ggtctggtct ttgccgaccg
    10021 cacgccgcat ccggcgctga cggaagcaaa acaccaacag cagtatttcc agttccgttt
    10081 atccgggcga accatcgaag tgaccagcga atacctgttc cgtcatagcg ataacgagtt
    10141 cctgcactgg atggtggcac tggatggcaa gccgctggca agcggtgaag tgcctctgga
    10201 tgttggcccg caaggtaagc agttgattga actgccagaa ctgccgcagc cggagagcgc
    10261 cggacaactc tggctaacgg tacgcgtagt gcaaccaaac acgaccgcat ggtcagaagc
    10321 cggacacatc agcgcctggc agcaatggcg tctggcggaa aacctcagcg tgacactccc
    10381 ctccgcgtcc cacgccatcc ctcaactgac caccagcgga acggattttt gcatcgagct
    10441 gggtaataag cgttggcaat ttaaccgcca gtcaggcttt ctttcacaga tgtggattgg
    10501 cgatgaaaaa caactgctga ccccgctgcg cgatcagttc acccgtgcgc cgctggataa
    10561 cgacattggc gtaagtgaag cgacccgcat tgaccctaac gcctgggtcg aacgctggaa
    10621 ggcggcaggc cattaccagg ccgaagcggc gttgttgcag tgcacggcag atacacttgc
    10681 cgacgcggtg ctgattacga ccgcccacgc gtggcagcat caggggaaaa ccttatttat
    10741 cagccggaaa acctaccgga ttgatggaca cggcgagatg gtcatcaatg tggatgttgc
    10801 ggtggcaagc gatacaccgc atccggcgcg gattggcctg acctgccagc tggcgcaggt
    10861 cgcagagcgg gtaaactggc tcggcctggg gccgcaagaa aactatcccg accgccttac
    10921 tgcagcctgt tttgaccgct gggatctgcc attgtcagac atgtataccc cgtacgtctt
    10981 cccgagcgaa aacggtctgc gctgcgggac gcgcgaattg aattatggcc cacaccagtg
    11041 gcgcggcgac ttccagttca acatcagccg ctacagccaa caacaactga tggaaaccag
    11101 ccatcgccat ctgctgcacg cggaagaagg cacatggctg aatatcgacg gtttccatat
    11161 ggggattggt ggcgacgact cctggagccc gtcagtatcg gcggaattcc agctgagcgc
    11221 tggtcgctac cattaccagt tggtctggtg tcaaaaataa taataaccgg gcaggctatg
    11281 tctgcccgta tttcgcgtaa ggaaatccat tatgtactat ttaaaaaaca caaacttttg
    11341 gatgttcggt ttattctttt tcttttactt ttttatcatg ggggcctact tcccgttttt
    11401 cccgatttgg ctacatgaca tcaaccatat cagcaaaagt gatacgggta ttatttttgc
    11461 cgctatttct ctgttctcgc tattattcca accgctgttt ggtctgcttt ctgacaaact
    11521 cgggctgcgc aaatacctgc tgtggattat taccggcatg ttagtgatgt ttgcgccgtt
    11581 ctttattttt atcttcggac cactgttaca atacaacatt ttagtaggat cgattgttgg
    11641 tggtatttat cttggctttt gttttaacgc cggtgcgcca gcagtagagg catttatcga
    11701 gaaagtcagc cgtcgcagta atttcgaatt tggtcgcgcg cggatgtttg gctgtgttgg
    11761 ctgggcgctg tgtgcctcga ttgtcggcat catgttcacc atcaataatc agtttgtttt
    11821 ctggctgggt tctggctgtg cactcatcct cgccgtttta ctctttttcg ccaaaacgga
    11881 tgcgccctct tcagccacgg ttgccaatgc ggtaggtgcc aaccattcgg catttagcct
    11941 taagctggcg ctggaactgt tcagacagcc aaaactgtgg tttttgtcac tgtatgttat
    12001 tggcgtttcc tgcacctacg atgtttttga ccaacagttt gctaatttct ttacttcgtt
    12061 ttttgctacc ggtgaacagg gtacgcgggt atttggctac gtaacgacaa tgggcgaatt
    12121 acttaatgcc tcgattatgt tctttgcgcc actgatcatt aatcgcatcg gtgggaaaaa
    12181 tgccctgctg ctggctggca ctattatgtc tgtacgtatt attggctcat cgttcgccac
    12241 ctcagcgctg gaagtagtta ttctgaaaac gctgcatatg tttgaagtac cgttcctgct
    12301 ggtgggctgc tttaaatata ttaccagcca gtttgaagtg cgtttttcag cgacgattta
    12361 tctggtctgt ttctgcttct ttaagcaact ggcgatgatt tttatgtctg tactggcggg
    12421 caatatgtat gaaagcatcg gtttccaggg cgcttatctg gtgctgggtc tggtggcgct
    12481 gggcttcacc ttaatttccg tgttcacgct tagcggcccc ggcccgcttt ccttgctgcg
    12541 tcgccaggtg aatgaagtcg cttaagcaat taatgtcgga tgcggcgcga gcgccttatc
    12601 cgaccaacat atcagggcgg aatgatcgca atgaacatgt cgatgagaga aagaataaaa
    12661 gcaggcaagc tatttaccga tatgtgcgaa ggcttacctg aaaaaagact tcgtggaaaa
    12721 acgttaatgt atgagtttaa tcactcgcat ccatcagaat ttgaaaaaag ggaaagtctg
    12781 attaaagaaa tgtttgccac ggtaggggaa aacgcctggg tagaaccccc cgtctatttc
    12841 tcttacggtt ccaacatcca tataggccgc aatttttatg caaatttcaa tttaaccatt
    12901 gtcgacgact acacggtaac gatcggtgat aacgtactga ttgcacccaa cgttactctt
    12961 tccgttacgg gacacccggt acaccatgaa ttgagaaaaa acggagaaat gtactctttt
    13021 ccgataacga ttggcaataa cgtctggatc ggaagtcatg tggttattaa tccaggcgtc
    13081 accatcgggg ataattctgt tattggcgcg ggtagtgtcg tcacaaaaga cattccacca
    13141 aacgtcgtgg ctgctggcgt tccttgtcgg attattcgcg aaataaacga ccgggataag
    13201 caatattatt tcaaagattt taaagttgag tcttcagttt aaataagcaa aattacctga
    13261 tgcgctccgc ttatcaggcc tacaatttca gtgatctacg taggccggat aaggtgttca
    13321 cgccgcatcc ggcataaact acgctcgcgg tagcccagcc gattaccgtt tgtaatcgat
    13381 ggcttctggc tgctccagat atacggtggt ttgtgccggt tgtgtgctgg caatcacctt
    13441 accgccacgt accgaataac gtaccggaac ctgacggcgc aacgcatcaa acccattttc
    13501 tgcaggcagg ataatcaggt tggcgctgtt tccggcggca atgccgtaat cctgcaaatt
    13561 caacgtcctg gcgctatggt gggtgattaa attcaggccg tcgttaatct gcccgtagcc
    13621 catcagctgg caaacatgca gccccatatg cagcacttgc agcatattcg ccgttcccag
    13681 cggataccac ggatcgaaga catcatcgtg accaaagcag acgttaatgc cggactccag
    13741 catctcttta acgcgcgtga taccgcgacg ttttggatac gtatcgaaac gtccttgcag
    13801 atgaatattg accaacgggt tggcgacaaa gttaataccg gacattttca gcaaacggaa
    13861 caggcgtgag gtatacgctc cgttatagga atgcattgcc gtagtatggc tggcggtgac
    13921 tcgcgcgccc atgccttcac ggtgcgccag ggcagcaacg gtttcgacaa agcgtgattg
    13981 ctcatcatca atctcgtcac agtgaacatc gatgagacgg tcgtattttt gcgccagggc
    14041 gaaggtttta tgcagcgact ccacgccata ttcacgggta aattcaaaat gcggaatcgc
    14101 cccaacaacg tctgccccta agcgtaacgc ctcttccagc aacgcttcac cattgggata
    14161 cgacaaaatc ccttcctgcg ggaaggcgac gatttgtaga tcaatccacg gcgcaacttc
    14221 ctgtttcact tccagcattg ctttcagcgc agtcagcgtt gcgtccgaaa catcgacatg
    14281 ggtacgcaca tgctgaatgc cgttggcaat ctgccatttc agcgtttgcc atgcgcgttg
    14341 tttcacatcg tcatgagtta ataacgcttt gcgctcggcc cagcgttcaa tgccttcaaa
    14401 cagcgtgccg gactgattcc agttcggttg tccggcggtt tgcgtggtgt ccaggtgaat
    14461 atgcggctcc acaaacggcg gtaaaactaa accttgttcg gcatccaggc tgttttcagt
    14521 tacgggcatc acaccagatt gcgtatcaat ggcgctgatt tttccgtcgt gaagatgaat
    14581 ctgccacagc ccctctttgc cgggtaaccg ggcgttaata attgtttgta aagcgttatt
    14641 cgacactgtt agcctccaca tgcgtcattg ctgctgtcgt tttacgattc aaaatcgggt
    14701 taaggatcag atagctcagc gcgccaccta atactgcgtt gaccggaaca attcccggta
    14761 accagtggcc tgcggcaatc cccagcgcga cagccagaat cgccacccaa ttgacactca
    14821 tcatacgcgt ggtcgcaaag tgctcatagc ggcgacggtt catcagataa tcagcgatga
    14881 tcacgccgcc cactggagga atagctgccg aaaggaaggt caaccagccg acaaaattgt
    14941 tatacagcca taatgcgcag accgtaccga taataccgtt aatcaccgaa agggttttgc
    15001 tcgacatacc ggtaatgttg gcgaaaccta aacccgacgc atagagcgcg ttatcgttgg
    15061 tggtccagat attcagcccc agcaccacaa tcgcaggcag cagcaggccc tgagcaatca
    15121 tcacatcaga gatatccgcc atccccagtg ccgcagcgcc cgctgcaccg aaaataaaca
    15181 tcaacgagtt gccgaggaaa aaggccacca tcgccaccag caccgccagt ttggcattgc
    15241 gaccaaaccg gacaaagtca gcggtgagcg tacccgcact gatgaatgac cccacaacca
    15301 gcgccagcgc gacattgaaa tctaacggtt gtgcgggaac gaccgctttt aatacgtcca
    15361 gtccgcccat gccgttaacg gccagccaca cggaataacc gccaaggcag gcgatagccg
    15421 gaaccgcaat caccgaaaga actgtcagcg ccgaaatgcc aaagaagacg gtgacggtca
    15481 tcagtaaacc ggagacggca atcagcaaat taatatccag cccggttgcc ttgcccaccg
    15541 gaatagaaaa catcgccacg ccaacgccaa accagccaac ctgggtgccg cccagcagca
    15601 gtgaaggcaa ccatgagcct ttaacaccaa acgagaagcg agcaagaaga tgggtggtca
    15661 ggccggtttt tgcgccaatg taaccaagaa atgaagtgta aataccgagg agaagattac
    15721 cgatgagaac tgcgaggaag aaatcatgat agctaagacc ggttccgaga gtgccgccgg
    15781 tccacatact ggcggaaaag aaggttaatc ccagcatgac gaacgtcaat gccaataccc
    15841 ctttccgcgc cgactgcggg actggcccct ggctaaagtt gttatcttgc gacacgaaat
    15901 tccccctttt tgctgtttga aacccaaaaa atccgccgca ttctattcat ctgaagataa
    15961 aaagcaatcg ttttcgtagg gggatatatt ttttatatga atgagtgcaa aaaggcgggg
    16021 gaaacttatc tgtgtaaaaa cagtgggtag gcatgataag acgcgtcagc gtcgcatcag
    16081 gcatctgcgc atggtgtcgg atgcggcgcg agcgccttat ccgacctacg gttcaggcct
    16141 gtaggcatga taagacgcgt cagcgtcgca tcaggcatct gcgcacggtg tcggatgcgg
    16201 cgcgagcgcc ttatccgacc tactcttcca tcgcttggcg gatctgatcc aacgacgccg
    16261 gatcatcaat ggtcgtcaga tcccccggat cgcgtccttc gcaaatcgcc tggatcgtgc
    16321 ggcgcagcat ttttccggat cgcgtttttg gcaattgcga gacaaaccag acgtgcgccg
    16381 ggcggccaaa gttgccaatc tggctgtcta ccagcgccat aattgccttc tcttgcaagt
    16441 gcgccacctc acggtcttcc agactgtcgc tctctttcgg aatgacaaac gccaccgcca
    16501 cctgcccttt cagcgcatct ttcaccccaa ccaccgccac ttcggcaacg cccggatgac
    16561 tggagatact ctcttcaatc tcacgcgtac ccagccgatg tccggcaacg ttaatcacat
    16621 cgtcagtgcg cccgagaata aagtgataac cgtcagcatc gcggatgccc cagtcaaaag
    16681 tggcgtacac cggacgggaa aacagcgacc agtacgtctt cacaaagcgg tcgtcgtcgc
    16741 cccagatggt ctgaatacag cctggcggca atggcccctc cactaccagc atccctttct
    16801 cattgacgcc acacggttcg ccggtgactt cattgagcaa ctgcacgtta tagccataca
    16861 tcggcacgcc ggggcttccc agacgcgtcg gcctgtcatc cagaccgcga gcaatcgcca
    16921 taatcggcca gccggattcg gtctgccagt agttgtcgat gaccggcaca tccagcgtat
    16981 tgctcaccca actggcggtc ggctcatcca gcggttctcc agccagatag agcacttcca
    17041 gcgacgaaag atcgtgtttg cgaatttcag cggtagggaa ttttttcagc acgcgaatgg
    17101 cggtcggcgc tgagaacatg cggctaacct gatatttctc gacaattttc caccacacgc
    17161 cgcagtccgg ccaggtcggc aatccttcgt aaacgatagt cgccatcccc gccagcagcg
    17221 gcgcgtaaac gatatacgaa tgccctacca cccagccgat atccgaagca caaaagaaca
    17281 cgccgcccgc tttgccgcca aaaatggtgt ccatcgaggt cgccagcgcc accgcatatc
    17341 cgccgacatc acgctgcaca cctttaggtt tgccggtcgt gccggaggtg tagagaatac
    17401 aggaggtttc gttggattcc agccacgcca ccggcacccg cgcaccgatg tgttgatggc
    17461 gcaacgacgc gaaatcgaca tcccgcccgc tgacgcgcgc cattttcgcc agtccgcgat
    17521 ccaccagcaa aacatggcgt ggctggtgtt gcgcctgact tatcgcatcg tcgagcaatt
    17581 ttttataggg aatgattttg ccaccgcgcg ctccggcatc agccgagaca atcagcaccg
    17641 gtttagcatc atcaattcgc gccgccacgc tgtgcgaggc aaatccccca aacaccaccg
    17701 aatgaatagc gccaatgcgc gcgcaggcca gcagggtaat atgcgcttcg gcaatcatcg
    17761 gcatatacac cagcacccga tcgccacgct gcacgcccag tgagcgcagc attgacgcca
    17821 ccgcattcac ttcgtcatgt aactggcgga aggtaaaggt acgctcttcc tctgtttccg
    17881 aagagacggc aatcaatgcc agcgcctctg gctgtttctc cagccagcga tcgatagcgt
    17941 tgtgacacaa gttggttcgg ccttcacaaa accaacgggc aaacggcggg ttgctgtggt
    18001 cgagcgtttg cgtaaagggc gtctgccagt caatacgccg ggcctgctcg gcccagaact
    18061 gctccggttc gttaatcgaa cgctgataaa attcgctaaa agacataatg ctctcctgtt
    18121 gaacttacgc cttaccgccg tttacttaaa tgacgtacag gtcgagatac tcattgaccg
    18181 gcatctgttc caggcgagtt ctgtcgagag aaacctccag aatgcgctgc tgctggcgag
    18241 tcgggaactg gcgcgcgata ttgattttga atttatcgac cagcttcgga atgccatcct
    18301 gacggcggcg agcatgacca atggggtact ccaccaccac ttcttcaaag cgcgtgccgt
    18361 cggtgaactc aagggttatg gcattggcga tggcgcgttt ttccgggtcg tggtagtcag
    18421 cggtaaacgc cggatcttca aagcaattga tcttctcgcg cagggcgtcg atgcgtttat
    18481 cttgcgcaac gttgtcctcg taatctgccg ccgttaagcg cccgaacagc agcgggatcg
    18541 ccaccatgta ctgaatgcag tggtcgcggt cagccgggtt attgagcggc ccttttttgt
    18601 cgatgatgcg aatacaggct tcgtgggtgc gaatggtcac tttttcgata tccgccgccg
    18661 ttttgcctgc tgcctgcatc tgttcataga gcgtcatcgc tgcttcaact gccgtctggg
    18721 agtggaactc cgccgggaag gagattttga acagcacatt ttccatcacg taagaaccat
    18781 acgggcgctg gaagcggaac gattcgcctt taaaggagac gtcgtagaag ccccacacag
    18841 gcgcagtcag ggctgacgga taacccattt cgccggtttt cgccatcagt gccagacgta
    18901 ccgcgcggga agtagcatcg cccgccgccc aggatttacg cgtgccggtg ttcggcgcat
    18961 ggcgataggt gcgcagcgac tgaccgtcca cccacgccag cgaaacggcg ttgagaattt
    19021 cctcgcgggt caggccgagc atttcggcga ccacggcggt ggaagccact ttcactaaca
    19081 ggacgtggtc gagaccaacg cggttaaagg agttttccag cgcgatgcag ccctgaattt
    19141 catgggcttt gatcattccg gtcagcacct gtttcatggt caacggcgct ttgccgctgg
    19201 cgaccgcgtt gcgcgaaagc cagtccgccg ttgccagaat gccgccgagg ttgtcggaag
    19261 gatgccccca ctccgctgcc agccaggtat cgttgaaatc gagccaacgg atcatcgcgc
    19321 caatgttaaa tgccgcctgg acggggtcga gctgaaactg agttccggga acgcgcacgc
    19381 cgttgggtac gacggtgccg gggacaattg gccccagcag ttttttacag gccggatatt
    19441 cgagagcttc cagaccgcag ccgagcgtgt cgagcaggca gtaatgtgcg gtgtcgtagg
    19501 ctactctgga gctgatttcg tagttcatca cgtaatcgac gatatcaacg atttcacgat
    19561 caaattccgg gcgaatgttg ttgatttgag ctgacattgg gtacgtttcc ttattgttat
    19621 tcgtagaggt ttactggcgc ttatccagcg cgacaaactg gcggtcttcc ggtccaacat
    19681 aattggcgga aggacggata attttgttgt cctgacgttg ttcgataatg tgcgccgccc
    19741 agccagtgac gcgggcgata acaaacagtg gtgtgaacat ctcggtggga acacccatca
    19801 tgttgtagga aacagcggag aaccagtcga gattggggaa catctttttg ctctcccaca
    19861 tcactgtttc caggcgatcg gcgatgttgt acatcttcag cgagccgcct tcctgcgaga
    19921 gctgctttgc cacgcgtttg atcacctggt ggcgcgggtc ggcgatggta taaaccggat
    19981 gcccaaaacc aatgaccact tctttgtttt ccacccgctt acggatatca gcttcggctt
    20041 cgtccggcgt ttcgtagcgt tgctggatct ccagcgacac ttcattcgcc ccgccgtgtt
    20101 tcggcccgcg cagtgcgcca atcgcgccaa taatggcgga atacatatca gagccagtgc
    20161 ccgcaatcac ccggctggta aaggtggaag cgttaaactc gtgttcggcg tacagcacca
    20221 gcgagatatg catcgccttt tcccagcttt gcgacggctt ttcgccgtgc agcagatgca
    20281 ggaagtgacc gccgatagag tcgtcatcag tttccggctg gatgcgttcg ccgttgtggc
    20341 tgtagtgata ccagtagaga agaatcgaac taagtgacgc cagcagtttg tcggcaatat
    20401 cccgcgcacc agaaacagtg tgcccctctt tttctggcag cgtgcagccg agcgcggaaa
    20461 cgccggtgcg cataacatcc atcgggtgtg acgccgccgg taaggcttcc agcacggtac
    20521 gcacgttagc cggtaaacca cgcagggctt tcagtttcgt tttgtaggcg gcgagttcgt
    20581 cacgggttgg cagtttgccg tggatcagca ggtgcgccac ttcttcaaat tcacaatgtt
    20641 ccgccagatc aagaatatcg tagccacggt aatgcaggtc gttgccgctt ttacccacgg
    20701 tgcagagcgc cgtattgccc gccggaacgc cggaaagtgc caccgatttt ttcggtttaa
    20761 tgacatgggt actgttttgc aggatcgttg tgtcgctcat attgtcctcg tcattgttat
    20821 catttgtagg gtcgagattg ttgtcggatg cgacgctatg cgtcttatcc gacctacagt
    20881 tcagttcacc tcgtaggcct gataagtcgc gtcagcggcg catcaggcat cggttgtcga
    20941 atacaaccaa cgggcgtttt tatttcacct gaccacgggc aaacaggtcg tcgagcttct
    21001 cttcgtactg gtagtagttg atgctttcgt acagctcgtt gcgggtctgc atggtgtcga
    21061 tgacgctttt ctgtgtgcct tcctgacgca ggatgttgta gacatgttca gcggcgcggt
    21121 tcatggcgcg aaacgctgaa agcgggtaga gcgccattgc gacatgggcg ctgcgtaatt
    21181 cgtcggtggt aaacagcggt gttgcgccaa attcggtaat gttggcgagg atcggcacct
    21241 tcaccgcatc ggcaaactgg cgatacatgg tgagttcggt aatcgcctcc gggaacagca
    21301 tttcggcacc cgcttcaaca taggcctccg ctcgttcgat tgccgcatcc agcccctcta
    21361 ccgccagcgc atcggtgcgc gccatgatca caaaatcagg atcggttttc gcatccaccg
    21421 ccgcgcggat ccgatccacc atctcttctt tcgagacgat cgctttattc ggacgatgac
    21481 cgcagcgttt cgcaccaacc tgatcttcaa tatgcaatcc tgccgcaccg gctttaatca
    21541 ttgatttcac cgtacgcgcc acgttaaagg ctgaagaacc aaaaccgata tccgcatcca
    21601 ccagcagcgg cagcgaacaa acgtcggtaa tacggcgaat gtcagtcagc acatcatcaa
    21661 gagtagaaat accgagatcg ggcagcccca gcgaccctgc cgccacgcca ccgccggaga
    21721 gataaatcgc ctgatatccg gcacgctgcg ccagcagcgc atggttagcg ttgatagtgc
    21781 caacaatctg caatggattt tctttagtca gtgcagcgcg aaacgcttta cctggagagt
    21841 gtagagacat agcccatcct ttgttatcaa cttgttattt gcgttgataa agacaaagca
    21901 atgggtgtgc cacaattgcc ttaataaaat taaacattta atttcaatga gttaaagata
    21961 aattcatgaa gtaatccgca aatatgcgtt tcagttaacg tttcaagcaa tgtttcaccg
    22021 cgtttcattg caacaattat gaaacaagac taaacccaat attcggtttc ttaactttgc
    22081 ggtgcgctat ggcacatcca ccacggctga atgacgacaa accggttatc tggacggtat
    22141 ctgtaacgcg cctgttcgag ctgtttcgcg atatcagcct cgagtttgat cacctggcga
    22201 atatcacccc tatccagctt ggctttgaaa aagcggtgac ctacatccgc aagaaactgg
    22261 ccaacgaacg gtgcgacgcc atcatcgctg ctggctctaa cggcgcatat cttaaaagtc
    22321 gcctgtcagt gccagttatt ttgattaaac cgagcggcta cgatgtgtta caggcactgg
    22381 caaaagccgg aaaactcacc tcttctatcg gtgttgtcac ctatcaggaa accattccag
    22441 cgctggtggc gtttcaaaag acctttaatt tgcgcctcga ccaacgtagc tacatcaccg
    22501 aagaagacgc ccgcgggcaa atcaatgagt tgaaagccaa cggcaccgaa gcggtggtcg
    22561 gcgcagggct gattaccgat ctggcagaag aagccggaat gaccggaatt tttatctatt
    22621 ccgccgccac cgtgcgccag gcattcagcg atgcgctgga tatgacgcgc atgtcgttac
    22681 gccataacac tcacgatgcc acccgcaacg ccctgcgtac tcgttacgtg ctgggcgata
    22741 tgctcggtca atcaccacag atggaacagg tgcggcagac tattttgctg tatgcccgct
    22801 ccagtgcggc ggtgttgatt gagggggaaa cggggacggg caaagagctg gcggcccagg
    22861 cgattcatcg ggaatatttt tcccgccacg atgcgcgaca gggcaaaaag tcacatccgt
    22921 ttgttgccgt taactgcggg gcgattgccg aatcgctgct ggaagcggag ctgtttggct
    22981 atgaggaagg ggcgtttacc ggctcgcgac gcggaggacg cgccgggctg tttgaaatcg
    23041 cccacggtgg cacgctgttt cttgacgaga taggcgaaat gccgctgccg ttgcagactc
    23101 ggctgctgcg ggtgctggaa gaaaaagagg tcacccgcgt cggcgggcat cagcctgttc
    23161 cggtggatgt gcgggtcatt agcgccactc actgcaatct ggaagaagat atgcagcaag
    23221 ggcagtttcg ccgtgacctg ttttatcggc tgagtatttt gcgtctacaa ctgccgccgc
    23281 tacgcgagcg ggtggcggat attctgccgc tggcggaaag ctttttgaaa atgtctctgg
    23341 cggcactctc tgtccctttt tctgccgcat tacgtcaggg attagagacc tgtcagattc
    23401 tattattgct ctacgactgg ccgggcaata ttcgtgaact gcgcaatatg atggaacgac
    23461 tggcgttatt tttaagtgtg gaaccgacgc cggatttaac gccgcaattt ttgcagctgc
    23521 tactgccgga actggcgcgc aagtcggcaa agactcccat tccaggcttg ctgacagcac
    23581 aacaggcact ggagaaattt aatggcgata agacagcagc ggcgaattat ttaggtatca
    23641 gccgaacgac gttctggcgg cggctgaaaa gctgaactgc cgaatgcagc gcgaacgctt
    23701 tatccggcat aaatttcaat agatgatgat caccgataaa cttacgcatc ggggatgttt
    23761 acataggatt cagaattact tcttcttata aatatttgcc gtgccgtgga tcttattgtc
    23821 agtttgacca gaggtcagta ccaacacatc agcccctttt tcatcggctt ttttgatcaa
    23881 atcttctttc gcatctgcgg tcgacatttc attactggtt gaaatatcac ctattttttc
    23941 atattgcgat gcaacctttt caaattccgc tttggtcatt aattctgcgg catagacatt
    24001 ggtaacggca aacgctaacg caccgactaa cattttagag attattttca tattacggct
    24061 ccagagttgt tatcacacta tcacccaccc acattattgg gttgattaat aattagttca
    24121 gtcagggcat aaacgccagt aaccggaaat aagacacttt gtgatctctc tcgcacccga
    24181 ttatttttgg tagaaatatg atgaagttac ttcacttatt tatggatgaa atcactatgg
    24241 atcctttgca cgccgtttac ctgaccgtag gattgttcgt gattactttt tttaatccgg
    24301 gagccaatct ctttgtggta gtacaaacca gcctggcttc cggtcgacgc gcaggggtgt
    24361 tgaccgggct gggcgtggcg ctgggcgatg cattttattc cgggttgggt ttgtttggtc
    24421 ttgcaacgct aattacgcag tgtgaggaga ttttttcgct tatcagaatc gtcggcggcg
    24481 cttatctctt atggtttgcg tggtgtagta gcatgcgccg ccactcaaca ccgcaaatga
    24541 gcacactaca acaaccgatt agcgccccct ggtatgtctt tttccgccgt ggattaatta
    24601 ccgatctctc taacccgcaa accgttttat tttttatcag tattttctca gtaacattac
    24661 atgccgaaac accaacatgg gcacgattaa tggcctgggc ggggattgtg ctcgcatcaa
    24721 ttatctggcg agtctttcta agccaggcgt tttctttgcc cgctgtgcgt cgtgcttatg
    24781 ggcgtatgca acgcgttgcc agtcgggtta ttggtgcaat tattggtgca attattggtg
    24841 tattcgcgct acgcctgatt tacgaagggg tgacgcagcg gtgagcagat aatttactct
    24901 ctacgtcatc aacctgattt gccgaatccc aaccatcgac gatgatagcc cccgtaatgc
    24961 gtggtcttta tttccgataa gtttacttga gtctttagct tcccatttca gccactctcc
    25021 cctgtgtggg ctttttcatt ggtctgaccc gtcttgaata gccaaacaat ggcaatgttc
    25081 cactttgcag ggatgataat aagaggatct gattcagaaa agttttgaaa agttggaccg
    25141 ccatcgaggc ctcgaacccc gtaccataca acaatcctgg ttgtcgtctg ctcttcctga
    25201 tgagctaatg gcggtttttg atatgagcct tacagcatat tttctgtcag gcttgagtgc
    25261 gatgcgtaag atacagaaaa gaccccacac aaacatgatg gaattatgat ttttttcata
    25321 tgaatgtcaa cccacacatt cacaaaacat ctgtatgaga acatttttta tacaccatat
    25381 agctgcgaaa ccactactgg tgctttttta agcgaaactc cgccgttcac aacaaaatat
    25441 cggcgaaaaa ggagggggaa tgccatttgg catacagata tacagatgaa atgatgacct
    25501 gctttgcgat tcactctctg gtggaataac aacttgcagg tggctctctt aaataaaaat
    25561 aaacgtcgca gataataaag ctctgattaa accagacact atcagaaatc gctcataact
    25621 aacgtgtaaa gtagtggagt taatttaaca atctgaattt acgctatagg tttaccacta
    25681 ctttgatatt atttcgctct ccttaactaa aaagttaagt ggagcgaagt taatatgtta
    25741 aaccacagga tgtttattaa ttttttcagt ctgttagtgt gcgattatcg ataacaaaac
    25801 gatatttcac atcaccacgc agcattcgct cataggcttc attaatttga tcggcccgaa
    25861 tcatctctat atcagccacg atgccatgtt cggcgcaaaa atcgagcatc tcctgagttt
    25921 ctggaatgcc gccaatcata gaaccagcta tcgcgcggcg tttcatgatc aggttgaaaa
    25981 cttccggcga tttatgcggt gtcgcaggcg caccaaccag cgttatggtg ccatcgcgct
    26041 tcagcaaggt ggtaaaatcg tcgagattat gtggcgcagc tactgtattc aaaatgaaat
    26101 cgaaactctt cacatgagcc accatctcat cggcattgcg tgagttaaca acttcatcgg
    26161 cccccagggc ttttgccgct tcgcgttttg actcagaagt ggtaaatgcc accacatgcg
    26221 cccccatcgc gtgggccagt ttaatcccca tatgtcccag accgccgatg ccgaccacgc
    26281 ccactttttt acccggcccg gcctgccagt ggcgtagcgg cgagtacgtg gtgattcctg
    26341 cacacaataa aggagccacc gccgccagct gctcttgcgg gtgacgaata cgcagaacat
    26401 atcgctcatg aacgacgatc tgttgtgagt agccgcccag agtatggccc ggttcatccg
    26461 gtgtcggcga gttataggtg ccggtcatgt aatcacagta gttttccagc ccgtcttcac
    26521 actcttcgca atgtttacaa ctgtcgacaa tgcagccgac accgaccaga tcgcccggcg
    26581 catgtttttc tacctgatca ccaacggcta ccacgcgccc cacaatttca tgacccggca
    26641 cgcaggggta aaccgtcccc gcccactccg aacggacctg gtggagatcg gaatggcaga
    26701 cgccacagta agcgatttcg attttgacat catgcggtcc cggttcacgc cgggtgatat
    26761 ccatcggttc aagcggttgt ttagcggaat atgcaccaac agctttgatc ttcattgtgt
    26821 ttactcctga ttagctatgt gtatttggcg atgcctgata tgtcatcgcg gcaaaacgcg
    26881 tccattgaat atagccaata tttaaaccac ccgatccagc cccaacatgc cgcgagcctg
    26941 aatgaaacag agcagcaacg ttccccataa ggcgagcgcc tataagtgtg atatttgcta
    27001 tccgtaaaag acaaaaatcg gcaaaactga tgatattttc acgtagacaa atagttatct
    27061 acacaacgtc aataagggca tattcttcaa agataatgtc catttctgcc aacacactca
    27121 tactcaggcc gccccactcc ctggggctta aatctcttac tgttcaccgt tgcagacata
    27181 ttaaatgcat atcaacctgc cacacttccc cacaccagtt gccctttatg gaacgtcgcc
    27241 gttctcggcg aaatacgcgc caccgcctcg gcggaacagg aggcgtccac cagcacaaaa
    27301 ctggcgtcat cctgcgcttt aggccacaca cgctcgcctt tttcattcag cggcaatata
    27361 tcgccagtgg ctaaaaacag cgagcgggag aggttctgtt cgttaggacg aatataaagc
    27421 tgcgcgtaca gattggcttt ttccagcatg tcgcccaggc cgtaaggcga ccagtggtcg
    27481 ataacgctgt cggtgccggt catcactttt acgcctttgt cgtgcagctg tttgagcggc
    27541 atatgcagcg tgccaatcgg caccgtcgaa gcgatcgaaa ttcgctgcgc cgccatccgg
    27601 tgcgccagtt catctacctg ttgttcgttg agcgttgcca gcgcaaaggc gtggctgatg
    27661 gtcagcttgc ccttcagctg tggcgttttc tccactgttt caaccatata attgatggct
    27721 gccacgcctg ccggcgtggt ttcgtgcagg tgaatatcga cgcctttgtc atagtccagc
    27781 gcaatctgga acatggtgtc gagggatttt tccatcgcgc catcaacact ggtcgggtcc
    27841 agcccgccga cgtaatgcgc ccccgcctgc atcgcttcgc gcattaagga ttccgatttc
    27901 gacagcagca aaccgtgctg cggaaaggcg acgatttcac acttgaagcc cgcctgacgt
    27961 cgcgccagca ccgcctgcaa attttgcaga tttttcaggc cggaaaccgg ttcgatattg
    28021 cagtggctgc gggcaatggt ggtgcctttc gactgcaata aatcgatcag tttttccgcc
    28081 cgttcctgcg tgtacggttg cagttccggc agcattttct gctcgagttt gatcatgtcc
    28141 tggatagtgg tgcctgccgg acgattgagc gagcgccacg gcccgccgta gaaggttttg
    28201 tcgagatgaa tatgcatgtc gcgggtggtg ggcagcatca gcttaccgcc agcgtcatag
    28261 tgcggcagcg tggcgtccgg atgctgcttg ttctcgcgca gggcaacaat tttaccgttc
    28321 tgaatttcca cagtctggcg cgccgtgcgg gtctgcaccg ccacgccatt ttcgtagtca
    28381 aaaccggttt ccagcagcac gttatcgaga tagtaatgcg ggtcagtgat tttcatggtg
    28441 ttgttcgctt cgcagtttag ggtgccagct ccccccgcat gggcgagcgg cactgaggga
    28501 ccaaacagcg cggcggcggt aaccatctta ccgctctggc tcagaaattc acggcggcta
    28561 ttgttttctt tcatcttacg tccttaggtt ttaataatat gggtgcccgt ttcgccgcgt
    28621 agcgccgcag gcaggcattc cggcgtggtg ataatcactt ctttgccgcc ttgttccagg
    28681 aacgtcaggc tggcgatgat ttttggcaac atgctgccag gtgggaaatg cccttcctgc
    28741 atatagcggg tcatggtggc aatatctacg cgatccagcg cctgctgctg cggtttgcca
    28801 aagtgaatac acactttttc gacgccagtg gtgatcacca gaatgtcggc gtgaatttcg
    28861 cgagccagta gcgcggtaga gagatctttg tcgataaccg cgtccacgct ttggtaatct
    28921 cccgcttcgg tgcgcaccac aggaattcca ccgccgcccg cgccaatcac cacaaagcct
    28981 tgttggatca gcgccttaat ggcaggtgct tcgacaatac gtttcggttc cggcgaggcg
    29041 accacgcggc gatagccccg tccggcatct tcaacaaaac gccagtcagg gtttgccttt
    29101 tgtagttcgt cacgctggct ttcactaaag aattcgccga tgggcttggt gggatgggca
    29161 aaccccggat cgtttttatc cacttccacc tgagtcacca cggtgacggc ttttttctcg
    29221 ccgtgacgcg ccagtcggtt gttcagcgcc tgctggatca gatagccaat gccaccttgc
    29281 gtatccgcca cacagttcgc cagcggcgtt aagggcagcc cttcgcgctc gtgggcaatc
    29341 tccgcgcggc gtaaatccag cccgacctgc ggcccgttgc cgtgggtcag cacaatgtcg
    29401 taatcggaag ccagcatttc cagcaccgta tcggccacgg ctttcaccgc ctccgcctga
    29461 tgctcaatcg actggctggc gttatctttg ataatgctgt tgccaccaat ggcaacgacc
    29521 acaagttctt tcatgttgtt atgtccgcta cagagggtga tggggttatg accaacgcca
    29581 gactgcctga tgcgctacgc ttatcaggcc tacagatttc cgcgccattc gtaggccgga
    29641 taagacattc atgtcgcatc cggcataaac atgtaactca gacgccgtaa cgttcgcacc
    29701 agccaaaaat ggctttctcg aagcaggcga agggtggatg cacaatgccc gcgccaatca
    29761 tgccgacgcc cgcatcttta tgggcgatgg cggtgttgat cactggcaga atgccgctgc
    29821 tacccacgcg ggtgatgtcg attgccgacg gaacgcccat aaaaccgagc agcggaatgg
    29881 tgacgttggg gttttcgcca agggtgattt cacgcatctg acgggagaaa tcgatagcct
    29941 cttccaccgt gccacccacc agcgcgacga tcgcgggcgc ggtcgccatc gcaaatccgc
    30001 cgataccgta ggtttcggtg atggcgctgt cgccaatatc cagccctgaa tcttccggct
    30061 tataaccggc aaacatcggg ccgatgacct gctgcgccgg gccggtaaac cactgccccg
    30121 gcaggccgct gacccgcagg ccgaactcga cgccgttacg cgccatggtg gtcaccacgg
    30181 tgctgtattc gatgccgtgc gccgcatcca tcgccgcttt acacatcgcc atccacgtcg
    30241 ggccggagaa gtagtcgctg ctggcgacaa actcaaacac ttcgcgctgt tgctcgacgg
    30301 aataacctgc ctgaataatc cccggcgtca gcgcctgaat cagcagcgtc gtcccggcgt
    30361 tattgcggtt atggcattca tcgcccatat gcagcgcctg cgccagcatt aagcgcagat
    30421 cgatttcgcc gataattttc atcgcgtcgc gcagcatggg tccctgcaca tcgcgcatcc
    30481 agttgaggcg gtcgatcacg ctctggtcgt tagcgcccat acgcaaaatc ttcgccatct
    30541 gctcgctcat gttggtataa gcgatgttgc cataggtttt gtttttcacg atgtgcataa
    30601 acatcgaggc cgaggtgacg cccgccatcg atcccacaca gtcgtgttcg tggcacggcg
    30661 agaaggtgat ctccccggaa gccgccagct cagccgcctc gtcgagatct ttcgccagtc
    30721 cttcaaacac cagcgctccg gtgaccgcgc ctttcatcgc gccgcacatt ttttcccagg
    30781 tgaccggcgg cccggcgtga agaatggttt tcggcgtcat gcccggcacc acgttaatcg
    30841 cctgatcaaa accaatcagc accggatgcg actggataat gcgctccagc gcctgctggt
    30901 tagcggcggt gattttgtcc gccagcggcg aatcggcaat gttgtccagt gcctgcacca
    30961 cctgcatatt gccctgcccc ggcggcgtcc agtcgagttg ggtcacttct acatgctgct
    31021 ttttcagatc atcgctaaac atggcgatac cgacgttaat aacgttcaac ggttggctaa
    31081 acagtgactg gctcatcagg cttcctcccc tttgcagata aattcacgcg ccagcaatcc
    31141 ggtattggtg ctgctgctcg ccagaatcac tccggcatca agcagcatct ggctttgttg
    31201 ttctaatgat ggcgtatcga gatcggtgcc cagcacatag gcgagaatga tcaactcgcg
    31261 tccttcggca gcggcgatcg ctttcgcttc tttgatcgcc tcgatggtgg aaccgaccgg
    31321 atcttcatgc gatccaaatc cgagcacaaa atccatcacg atcaccgcca cttctggatc
    31381 gcgcgcttct tcgatcaagc ggctgatacg gttggtcggg tcaatcattg gatgcggctt
    31441 gccgttggtg aagtcatcat cgccaaagtc gaggaaggtg tgtttgatgc tgcggttgat
    31501 atcttgcagg cggaattcag gatcgggctg gatattgctg tagacatcgc catgtttttc
    31561 catcaccgcg aacagggttt cgtcacacag cgtaccgccg cagaacagac cacgaatgta
    31621 tttctgctgc ggttgcagac gcgcccgcac atccgcaatc aacggctggt taagcgtatg
    31681 caggtcgaga tgttcttgtt tcacgccgga gagcatcacc gctttcaatg ccgcctcttt
    31741 gctgccgcga gcaaactgta gcccctgctc atccactggc gtttcgccac gaccgaggaa
    31801 gcaggccacc actggcttgc ggcaggcgcg tgcgcgttcc agcactttgc gggccaccgc
    31861 aggcgcaggt ggtttggaga taagcacaat gatttcagtt tgcggatcgt tttccagcat
    31921 cccgatggca tcgagcatca tcaggccacc gattttctcg ctcaggtcgc gcccgccggt
    31981 gccgatcagt tgtgaaacgc cgccaccaaa ttcatggata cggacgctca attcctggct
    32041 gccggtgcca gatgcgccaa caataccgat gttgccgcga cgcacggcgt taccaaagca
    32101 gagcgccgcg ccgttgataa tcgccgtgcc gcagtctggc cccatcatca gcagcccttt
    32161 ttcatgggcc aactgtttga gcgccagttc atcttcaact gagacattat cggaaaacag
    32221 catcacgttg aggtcatttt gcagcgcctg acgcgcttcg cgagcggcaa acagaccgtt
    32281 gaccgaaatc actgccaggt tactttccgg gatatgcttt ttggcgctgg caatagtggc
    32341 gtaacgcgcc tcgtgcgagc cgctttgcgc tttggtgttg aacagttctt caatctccgc
    32401 cagcaactgc tcgttgtcca cacccgattt accattgatg acaatcatca ggtcgccgtt
    32461 tttcgcttgc tccagctccg gcgtcagcag tcctaaattc ttcaacacgc ctttgttcat
    32521 ttcggtcgcc atcgccacaa atgcctgctc gacgccgtcg agtttatttg cacgcgtgga
    32581 gatagacatc agcgagacag aatcaaaata ggtattcggt ttaatgacta ttttaactga
    32641 cataattccc tccgtaagtt tggctcagcg cacaaccatc ggccatgcca tacagcatgt
    32701 cgcagccgga actggagcca atatgtttaa ttttttctat tgcctgagtt gcgttcccag
    32761 ggatgtcata aataatgttg tgaataaaac gatgaatatt ttcccggcag cgttgttgta
    32821 atgcggcttc cagcgtgatg gcacttaata acgtggtatt atttctgccg cgttgcaaac
    32881 cgagataaaa ttcctctttg tatttttccg ccggatgtcc ggaaataaat aaaataagta
    32941 ccagtccggt cagataatca tcggctgatg gcgtaaggcc aatccctaag cccatcatac
    33001 tggcgaccgc tacggcgata ttttgtttat cgtttacggc ctgaataact gcccgacgtt
    33061 taatatgtag ttctttattt aacgcctgat aaaacggatt atcgccgcga taattaaaca
    33121 gggtttcatt ttcccccagg tgctgatgaa taatgtcgtg ccactgttgc caggcgataa
    33181 ggtgaaaatt cagagcggtc atttgcaagg ttggggacag ccaacgctgg caatgactca
    33241 tctctatatg atgatgttga ccaatcgtaa tgccttgatc gtcaaactga accttatcac
    33301 catgccggaa cagaccatta aagtgagtga gtgccaaccg acaactgtta ggcgcattgt
    33361 cgtactcttc acaaagcagc gtcagcaact gctggcgagc cgggagtacg atatttaccg
    33421 ctctggcaaa gacctgttca acctgtcctt gcccccgctg cgccagaaaa tccgcatccg
    33481 cagttagcgc ccacacacac cgtctttctg gtgtgttgat agcaagcctc ccggtttatg
    33541 cgcctgcggc aatcagtaac tgcgcaatct cttcaaagcc ccgttctcgc gccagttcca
    33601 gcggtgtttt gccatattta tccgtcagat gcgggctggc accgtgttcc agtaataact
    33661 ggacaatcgc ctgctgttta atcccgccat cattaagcac aatcgcttcc agtagcggcg
    33721 tccagccaac atggttggtc tggttaacgt taatctccgt atgcgccaaa agctctttta
    33781 caatacttaa atggcctttt tcacaggcag gcgtcaggcc gacaccgcca aaacgggtta
    33841 cgcaattaag atcgggttta gccggtaaaa taattcgtag tagcgttaaa tcatcgttca
    33901 gacagctaat taaaaaagga tttaaacagg tatgatcttg tttattaata tccgctccgg
    33961 cgtcaattaa tgcctgaacg caagcatatt gctgatataa acttgccagc gtaattgccg
    34021 ttttcccgtg gcgatcgcag gtattaatat agacacccag cgcaaggcag gtttttactt
    34081 tatcgatatc cccctgttgc gcagccaata aatagtctgc aggtggattt tttatagtca
    34141 ttcacattca cctggttttt gtggctgttt tcaatgaatt gagaatagca acgataatct
    34201 gataaaagaa cccgcttaaa tttaattcgc cgtatttcgc ctttttgttg aatgatgttc
    34261 aacaatcttt tcgccgtgtg cggtagtgca atatttgttt ctttgacccg atacactttc
    34321 actcaatcag gttttatttc tctatagtct gcacatgtaa gagtagcttt cgtccatgtc
    34381 taaccagagg ggaacaagat gaacggactc acggcaacgg gtgtcaccgt cggtatttgt
    34441 gcagggcttt ggcagttagt gtcttcacat gtcgggctct ctcagggatg ggaattatta
    34501 ggaaccattg gcttcgtcgc cttttgcagt ttttatgccg caggcggcgg taaatcgggg
    34561 tttatcagaa gcctggcggt taattattcc gggatggtgt gggcgttttt cgccgcgctg
    34621 gctgccggtt ggctggcccc ggtgagtggg atttccgggg tctgggcgag cgtaattacg
    34681 actgtaccct tctcggcggt ggtggtctgg caagggcgtt tctggttact gtcttttatt
    34741 cccgggggct ttctcggcat gacgctattt tttgccagcg ggatgaactg gacggtgaca
    34801 ttactgggtt ttctggcggg taactgcgtg gggataattt cagaatatgg cggacaaaaa
    34861 ttaagcgaag cgacgactaa acgcgacggg tattaaaagt tattcaaaaa tgattaccca
    34921 tctttaattg atacctctac agttatgcct ggagaaattc atattagata atggcgggaa
    34981 tggtttaatg aactcaattt ttaccgaaga gaatctgttg gcatttacta ccgctgcgcg
    35041 ttttggcagc ttcagtaaag ccgcagaaga gttgggttta accacttccg ccattagcta
    35101 caccattaaa cgtatggaga cggggctgga tgtggtgctg ttcactcgca gtacccgcag
    35161 cattgagtta acggagtccg ggcgttattt tttccgcaaa gctaccgacc tgcagaatga
    35221 ttttcatgcc attaaacgca gtatcgatac tatttcgcag ggcatagaag cgcgtgtgcg
    35281 catttgtatt aatcagctgc tttatacgcc caaacacacc gcacgcttat tacaggtgct
    35341 gaaaaagcag tttcctacct gccagattac cgtcaccacc gaagtgtata acggcgtctg
    35401 ggatgcgatt attaataatc aggcaaatat cgctatcggc gcccccgaca cgctgctgga
    35461 cggtggcggc attgattaca cggagattgg cgcgattcgc tgggcttttg ccatcgcccc
    35521 ggatcaccca ttagcctttg tgccggagcc gatcgccgaa agccagctgc gtctctaccc
    35581 taatatcatg gtggaggaca ccgcgcatac gattaataaa aaagtgggct ggttgctgca
    35641 tgggcaggag tcaattctgg tgcccgattt caacaccaaa tgtcagtgcc agattttagg
    35701 tgaaggaatt ggttttttgc cggattacat ggtccgtgag gcgatggcgc aatccttgct
    35761 ggttacccga caaatccata acccgcgcca ggattcacgc atgttactcg caacccagca
    35821 ctctgccacc ggtcaggtga cgcagtggat taaaaaacaa tttgcgccaa atggcatatt
    35881 aacggggatc tatcaggatt tactgcatcg ggagaattaa tcgctgccaa cctcccgatg
    35941 cgctgtgaat agcggtttac atcaaccacc tgctttcatt acccattcag agataaattt
    36001 attacccggt actggcggag agtacaaata gccctgtaaa aagtgaacgc ctttaccgat
    36061 cattaaatcc gcctgctcct gggtttctac cccttccgcc acgatactca gaccaggctt
    36121 acgcgccagt tcgacaatat tgtccacaat atgaccggag atttcatcga cactcgccat
    36181 ttgcacaaac gacttgtcaa ttttaataaa atcgaccggg aacgcctgca agtaacgata
    36241 ggtcgcataa cccgtaccaa agtcatccag cgcaaaagta atattgtgct gatgaaggct
    36301 gtcaaatatc gctctggctt ctggcgttac cggaatagga ttacgttccg tcagctcaag
    36361 aaccagcttg attttatcgt tacctaattt cttaaccagg ttcagacact ctttttcaaa
    36421 tcccgcagcc aaaaaacagc ccgccgagac gttgatacca atatggaaat tgtccggcag
    36481 caaatgtttt accggcatca gaatatccgc agtctgtttc atcagctggc gagtcattat
    36541 gacaataaga ccggatgact ccgccagagg aataaactga tccggtggga tcattcccgt
    36601 ttgtggatgt tcccagcgga caagcacttc acagcccgtc agtaccccag tctgcgcgca
    36661 gaaaaccggt tggatccacg gtttaaactc gtggttttcc aacgccagac tgatggcttc
    36721 aggcgtgacg atatggtgat agtgattatc attaatatat ttgtgactat tattacccgt
    36781 ggcggatatg atctccggat tgtactgaaa gaaaatatcc cgccagaaag taatatcgct
    36841 ctgaatcccc agtttctcaa agagctgttt cttttgatgt gaaatggtct ttgcactgcg
    36901 atttctgtac tgtgatattt ctgttacaga cattccctgc atcagcagct tgagtagcag
    36961 tcgttctttc tcggatacgc taccaggcaa tgacaaatga accgttgtac cgaactcttg
    37021 cagaaaaaca cgaaaatcac atgaattcat gaacacacct tttatctttt atcgctgttc
    37081 agtatgagtg attacctgtg gattcctgag cacacataag gggtaaccat aaacattagc
    37141 gatgccatac gtataacatc ccacatcact atcaagaaga ttatctttac ctctgatgct
    37201 cgctcaaaaa cagttacgct aaatacctta aaaaggtaat cacaagtcaa actcaggaga
    37261 taatcacgca agaatgcaat attaaaattc agatactcat ttttggtaat aaagaagtag
    37321 ttggactatt tcgatattta taatttccca ctacgtattg acgcccattc atgagtaaag
    37381 gcgcattata tgaatttaac aatccagatc aactgaaaat acctcaacct cataaacaca
    37441 tagcgtcaac attcaatgac ataatgagta aagatgttgg ttatgcatac acgtcattac
    37501 tctatgccta tcccttaaca acctgcttct taagactgaa tccattcagc aatagatagc
    37561 caacattata acaatgacaa taaaaacaat atttaaaaca atcaataaaa tatcagacac
    37621 agacaattaa accttaaaca acaaacggtt acattgattt tagttgattt gcgctacgcg
    37681 ttccccatga ataaagcgtc atttcaaatt agcaccaata tttacattaa ataagactaa
    37741 gcttattgaa cattcacaac agtccaaacg taggttttgg gcttcaatag ttcttttctt
    37801 ttttattatc tgttgcctgg caatcatcaa tcgatactaa gggcttaact gtacacagat
    37861 ataacgtatc aagaggtaag agaaaccgcg ccatatgcag acatggcgcg gttttatgca
    37921 ataacaatca cgagtccggg aacatcacgc tatggcccgg cgcttcacga tggagatgga
    37981 taaagttaag gtgccgctcg tactggtcga gaatatcggt gatcacctgc tctttgctgt
    38041 agtccatcag gtcgttgccc tggctgcctt ctaacaggaa ggtttccagc cggtagtagg
    38101 tcgatttccc gctacgtgcg cggtaggtaa agcccggcac tgaatattgc tgcggccaaa
    38161 tctgatagac aaagttctgc tcttcgccca tatgcaccaa caaatccaga tgccccaact
    38221 gttgtccctc ttccggcggc aggtttttta gctccacgta cgcgccgcgc aaccgcagtt
    38281 cctgcgccac ttcttccatt gccggataac agaccgtctc catcatctgt ttagtgtaac
    38341 gcgtgcccgg ataattcatc aggcgcgaga gacgtttttt ccagctcagg cgatcctgaa
    38401 gccccagcgg tcgcggcgcg gtatcgcggt tggcactttc acggcggtaa tcttctacct
    38461 tcagagattt atacaacccc gccatcacga agaagatcac aaagctgaac ggcaggccca
    38521 taatcaccgt ggtgttttgc agcgcagata tcccgttggt catcagcatg cctagcgtca
    38581 gcaggccaat cgccaccgac cagaagacgc gcagccagcc gggtgcgtcg ctgttgatat
    38641 ctttaagctg cgaggtgaaa ttccccagca ccaacgcacc ggagtccgcc gaggtcacat
    38701 aaaacagcag gccagtaatg gtggcgacgg aggcgctaaa ggtaaacgcc ggatactgcg
    38761 ccagcaggct gtagaagccg cgctccggat ggaccatcgc ttcctcggca aatgcagcgc
    38821 cgccgtggat gatttcatac agcgcgctat tgccgaatac cgagagccat aacagcgtga
    38881 aggtaaacgg aataatcaac gtgcccagca cgaactggcg aatggtacgc ccacgcgaga
    38941 tacgcgccag gaacaagccg acaaacggcg accatgccac ccaccatgcc cagaagaaga
    39001 gcgtccagtt attcatccac tcaaccggac ggtcgaaggc aaaactgttg agcgtcatgc
    39061 ccataaagcg attcacatag tcgccaacat tcagcaccag tgcattaagc aggaacgagg
    39121 tgtcgcccat aaacaacacg aacagaatca atcccagcgc cagcgcaacg ttgagttccg
    39181 ataacacgcg aatgccctta tcaacaccgg atgtcaccga gatagtggcg atgatcaccg
    39241 acaaggcgat cagtgccgct ttcgccgcca tcgaatcggg aatatcaaac agtacgctca
    39301 agccatagtt aagctgcacc acaccgatac cgagcgtagt ggcaataccg aagatggtgc
    39361 cgatcaccgc agcaatatcc actgagtgac ctatcggccc gttaatccgt ttaccgaaga
    39421 tcgggtacag cgccgagcgg atagtgagcg gcaaattata acgatagcta aagtatccga
    39481 gcgccatgcc catcagcgca tacatcgacc agccggttaa gccgtagtga aacagcgtcc
    39541 agaccatcgc ctgacgcgcg gcctcaatcg tctgtcccgc gccttccggc ggctgcatat
    39601 actgcgttac cggttcggct acggagaaga acatcaggtc gataccgatc ccggcagcaa
    39661 acagcatcgc cgcccaactc agcagactga attccggttt tgattgttca ggcccgagct
    39721 tcaccgaacc aaaacgcgaa caagcgatac agactacaaa gacaatatag agcgttgccg
    39781 ccagcagata gtaccaaccg aaggttttag agacccagtc cagcgtgcgg ccaatccaca
    39841 gggccgagaa gtcgcgaaac aggatcgttg tcagggaaaa caacaaaatc agtccggcgg
    39901 aagtataaaa caccaccgga ttgattttgt ccttttccct gctgtgtgaa aggtctgtca
    39961 tccagtatcc ccactgttat tgttaccatt taaaatcaaa ttagtaacaa ttaagacaca
    40021 ttttatattg aacgtccaat caataaccgc tttaatagat aaacaacgct gatgaatgga
    40081 gtggcgaaaa tgcccaaatt gggaatgcag tcgatccggc gcagacaact gatcgacgcc
    40141 acgctggaag caataaatga agtgggcatg cacgatgcaa cgatcgcgca gatcgcccgc
    40201 cgtgcaggcg tttctacggg gatcatcagc cactatttca gggacaaaaa tggtctgctg
    40261 gaagcaacca tgcgcgatat caccagtcag ctgcgtgacg cggttttgaa tcgattacat
    40321 gcacttccgc agggcagtgc agagcggcga ttacaggcga ttgttggcgg aaacttcgat
    40381 gaaacccagg tgagcagtgc ggcgatgaaa gcctggctgg ccttctgggc cagcagtatg
    40441 catcagccga tgctctatcg cttgcagcag gtcagtagcc gccgcttatt gtcgaatctg
    40501 gtgagcgaat ttcgccgcga gttaccgcgc caacaggcac aggaagcggg ctacggcctt
    40561 gccgcgctaa ttgacggatt atggctgcgc gcggcactga gcggcaaagc gctggataaa
    40621 cctctcgcac actcgctgac ccgtcacttt attactcagc atttacccac cgattaaccg
    40681 aggagacgtg atgtcccgaa tggcagaaca gcagctttat atacatggtg gttatacctc
    40741 cgccaccagc ggtcgcacct tcgagaccat taacccggcc aacggtaacg tgctggcgac
    40801 cgtgcaggcc gccgggcgtg aggatgtcga tcgcgccgtg aagagtgccc aacaagggca
    40861 aaaaatctgg gcggcgatga ccgctatgga gcgctcgcgt attctgcgtc gggccgttga
    40921 tattctgcgt gaacgcaatg acgaactcgc aaaactggaa acccttgaca ccggaaaagc
    40981 atattcggaa acctcaaccg tcgatatcgt taccggtgcg gacgtgctgg agtactatgc
    41041 cgggctgatc ccggcgctgg aaggctgcca gatcccgttg cgtgagacgt catttgttta
    41101 tacccgccgc gaaccgctgg gtgtggtggc ggggattggc gcatggaact acccgatcca
    41161 gattgctttg tggaaatccg cccccgcgct ggcggcgggt aacgcgatga tcttcaaacc
    41221 gagcgaagtc accccgctta ccgcgttaaa gctggctgaa atttacagcg aagcgggcct
    41281 gccggacggt gtatttaacg tgttgccggg cgtgggcgcg gagaccgggc aatatctgac
    41341 cgatcatccg ggcattgcca aagtgtcatt taccggcggc gtcgccagcg gcaaaaaagt
    41401 gatggctaac tcggcagctt cctccctgaa agaagtgacc atggagctgg gcggtaaatc
    41461 gccgctgatc gtttttgacg atgcggatct cgatctcgcc gccgatatcg ccatgatggc
    41521 gaatttcttc agctccggtc aggtgtgtac caacggcact cgcgtcttcg ttccgacgaa
    41581 atgcaaagcc gcatttgaac aaaaggttct ggcgcgcgtt gagcgcattc gcgcgggcga
    41641 cgttttcgat ccgcaaacta acttcggccc gctggtcagc ttcccgcatc gcgataacgt
    41701 gctgcgctat atcgccaaag gcatagagga aggcgcgcgc gtactgtgcg gcggcgatgt
    41761 actgaagggg gatagcttcg ataacggcgc atgggttgca ccgaccgtgt tcaccgattg
    41821 cagcgacgat atgaccattg tgcgcgaaga gattttcggg ccggtgatgt ccattctgac
    41881 ctacgagtcg gaagacgaag tcattcgccg cgctaacgat accgactacg gtctggcggc
    41941 gggtatcgtg acggcggacc tgaaccgcgc gcatcgcgtc attcatcagc tggaagcggg
    42001 tatttgctgg atcaacacct ggggcgaatc cccggcagag atgcccgttg gcggctacaa
    42061 acactccggc attggtcgcg agaacggcgt gatgacgctc cagagttaca cccaggtgaa
    42121 gtccatccag gttgagatgg ctaaattcca gtccatattt taaccaggag gtttatttgc
    42181 aatttgacta catcattatt ggtgccggct cagccggcaa cgttctcgct acccgtctga
    42241 ctgaagatcc gaacacaacc gtgctgctgc ttgaagcagg cggcccggac tatcgctttg
    42301 acttccgcac ccagatgccc gccgcgctgg cgttcccgct acagggcaaa cgctataact
    42361 gggcctatga gacggaaccc gaaccgttta tgaacaaccg ccgcatggag tgcggacgcg
    42421 gcaaaggtct gggcggttca tcgctgatca acggtatgtg ctacatccgt ggcaatgcgc
    42481 tggatctcga taactgggca caagaacctg gtctggagaa ctggagttat ctcgactgcc
    42541 tgccctacta ccgcaaggcc gagactcgcg atgtgggcga gaacgactac cacggcggtg
    42601 atggcccggt gagcgtcacc acctccaaac ccggcgtcaa tccgctgttt gaagcgatga
    42661 ttgaagcggg cgtgcaggcg ggctacccgc gcacggacga tctcaacggc tatcagcagg
    42721 aaggttttgg cccgatggat cgcaccgtca cgccacaggg ccgtcgcgcc agcaccgcgc
    42781 ggggttatct cgatcaggcc aaatcgcgcc ctaacctgac cattcgtact cacgccatga
    42841 ccgatcacat cttttttgac ggcaaacgcg cggtgggcgt cgagtggctg gaaggcgaca
    42901 gcaccattcc gacccgcgcg gcggcgaata aagaagtgct gttatgtgca ggcgcgattg
    42961 cttcaccgca gatcctgcaa cgctccggcg tcggcaacgc tggactgctg gcggagtttg
    43021 atattccgct ggtgcatgaa ttacctggcg tcggcgaaaa tcttcaggat cacctggaga
    43081 tgtatctgca atatgagtgc aaagaaccgg tttccctcta ccctgccctg cagtggtgga
    43141 atcagccgaa aatcggtgcg gagtggctgt ttggcggcac cggcgttggt gccagcaacc
    43201 actttgaagc aggcggattt attcgcagcc gtgaggaatt tgcgtggccg aatattcagt
    43261 accatttcct gccagtggcg attaactata acggctcgaa cgcagtgaaa gagcacggct
    43321 tccagtgcca cgtcggatcg atgcgctcgc caagccgtgg gcatgtgcgg attaaatccc
    43381 gcgacccgca ccagcatcca gcgattctgt ttaactacat gtcgcacgag caggactggc
    43441 aggagttccg cgacgcaatt cgcatcaccc gggagatcat gcatcaaccg gcgctggatc
    43501 agtatcgtgg ccgcgaaatc agccccggtg tcgaatgcca gacggatgag cagctcgatg
    43561 agttcgtgcg taaccacgcc gaaaccgcct tccacccgtg cggtacctgc aaaatgggtt
    43621 atgacgagat ggcagtggtt gacgccgaag gccgcgtaca cgggctggaa ggcctgcgcg
    43681 tggtggatgc gtcaattatg ccgcagatta tcaccggcaa tttgaacgcc acgacgatta
    43741 tgattggcga gaaaatggcg gatatgattc gcgggaagga tgcgttgccg aggagcctgg
    43801 cggcgtattt tgtggcgaat gggatgccag taagagcgaa aaaactgaat cgcgatttga
    43861 actaacgcag gaacttacaa gattgagagt taccgttcta cgatgaggac cgaatcctta
    43921 tataaaaaac ataggtaact ctcatgcttc atatccgcag tcccgccatc aaactttaag
    43981 ccgattcgct caatctggcg gatgatggtg ctatggatac gctaataaac cacaagtgta
    44041 atgccactca gcttaagaat cacgcaacca gtgctcctgg ctagggttac ttgttctttc
    44101 ccactcccgg ttagcagtgg tcagcgatca actgcgtaag ccgggcgtat ttatcccaaa
    44161 gcgtggagta atatcaggtc tacgctaaaa aagtcaattt attcggttat catgcacgaa
    44221 aaggggactt gtggcgagta taaaaataag tcagacccaa aaactacgtg gagctttaaa
    44281 cacggttcgt ataatttat
//
LOCUS       DADSYM010000015        42970 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2415
            SAMN08039579-rid11723173.denovo.015, whole genome shotgun sequence.
ACCESSION   DADSYM010000015 DADSYM010000000
VERSION     DADSYM010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039579
            Sequence Read Archive: SRR6362533
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 42970)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 42970)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980162.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 03:39:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,484
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 4,347
            CDSs (with protein)               :: 4,347
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 43 of 85
            Pseudo Genes (incomplete)         :: 37 of 85
            Pseudo Genes (internal stop)      :: 19 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42970
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039579-rid11723173.denovo.015"
                     /strain="LGE2415"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8302 N 95.9628 W"
                     /collection_date="2015-08-24"
     gene            190..1254
                     /gene="ulaG"
                     /locus_tag="J1Y83_000708"
     CDS             190..1254
                     /gene="ulaG"
                     /locus_tag="J1Y83_000708"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003856017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HBA7628890.1"
                     /translation="MSKVKSITRESWILSTFPEWGSWLNEEIEQEQVAPGTFAMWWLG
                     CTGIWLKSEGGANVCVDFWCGTGKQSHGNPFMKQGHQMQRMAGVKKLQPNLRTTPFVL
                     DPFAIRQIDAVLATHDHNDHIDVNVAAAVMQNCADDVPFIGPKTCVDLWIGWGVPKER
                     CIVVKPGDIVKVKDIEIHSLDAFDRTALITLPADQKAAGVLPDGMDDRAVNYLFKTPG
                     GTLYHSGDSHYSNYYAKHGNEHQIDVALGSYGENPRGITDKMTSADILRMGEALNAKV
                     VIPFHHDIWSNFQADPQEIRVLWEMKKDRLKYGFKPFIWQVGGKFTWPLDKDNFEYHY
                     PRGFDDCFTIEPDLPFKSFL"
     gene            1362..2117
                     /gene="ulaR"
                     /locus_tag="J1Y83_000709"
     CDS             1362..2117
                     /gene="ulaR"
                     /locus_tag="J1Y83_000709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator UlaR"
                     /protein_id="HBA7628891.1"
                     /translation="MTEAQRHQILLEMLAQLGFVTVEKVVERLGISPATARRDINKLD
                     ESGKLKKVRNGAEAITQQRPRWTPMNLHQAQNHDEKVRIAKAASQLVNPGESVVINCG
                     STAFLLGREMCGKPVQIITNYLPLANYLIDQEHDSVIIMGGQYNKSQSITLSPQGSEN
                     SLYAGHWMFTSGKGLTAEGLYKTDMLTAMAEQKMLSVVGKLVVLVDSSKIGERAGMLF
                     SRADQIDMLITGKNANPEILQQLEAQGVSIMRV"
     gene            complement(2114..2863)
                     /gene="yjfP"
                     /locus_tag="J1Y83_000710"
     CDS             complement(2114..2863)
                     /gene="yjfP"
                     /locus_tag="J1Y83_000710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HBA7628892.1"
                     /translation="MIEIESRELADIPVLHAYPVGQKDTPLPCVIFYHGFTSSSLVYS
                     YFAVALAQAGLRVIMPDAPDHGSRFSGDAARRLNQFWQILLQSMQEFTTLRAAIAEEN
                     WLLDDRLAVGGASMGAMTALGITARHPTVKCTASMMGSGYFTSLARSLFPPLIPETAA
                     QQNEFNNIVAPLAEWEATNHLEQLGDRPLLLWHGLDDDVVPADESLRLQQALSETGRD
                     KLLTCSWQPGVRHRITPEALDAAVTFFRQHL"
     gene            3045..3374
                     /gene="bsmA"
                     /locus_tag="J1Y83_000711"
     CDS             3045..3374
                     /gene="bsmA"
                     /locus_tag="J1Y83_000711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313192.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm peroxide resistance protein BsmA"
                     /protein_id="HBA7628893.1"
                     /translation="MVSRKRNSVIYRFASLLLVLMLSACSALQGTPQPAPPVTDHPQE
                     IRRDQTQGLQRIGSVSTMVRGSPDDALAEIRAKAVAAKADYYVVVMVDETIVTGQWYS
                     QAILYRK"
     gene            3523..3798
                     /gene="yjfN"
                     /locus_tag="J1Y83_000712"
     CDS             3523..3798
                     /gene="yjfN"
                     /locus_tag="J1Y83_000712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418609.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease activator YjfN"
                     /protein_id="HBA7628894.1"
                     /translation="MKQLLASPSLQLVTYPASATAQSAEFASADCVTGLNEIGQISVS
                     NISGDPQDVERIVALKADEQGASWYRIITMYEDQQPDNWRVQAILYA"
     gene            complement(3915..5540)
                     /locus_tag="J1Y83_000713"
     CDS             complement(3915..5540)
                     /locus_tag="J1Y83_000713"
                     /EC_number="1.3.8.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418608.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isovaleryl-CoA dehydrogenase"
                     /protein_id="HBA7628895.1"
                     /translation="MHWQTHTVFNQPIPLNNSNLYLSDGALCEAVTREGAGWDSDFLA
                     SIGQQLGTAESLELGRLANVNPPELLRYDAQGRRLDDVHFHPAWHLLMQALCTNRVHN
                     LAWEEDARSGTFVARAARFMLHAQVEAGSLCPITMTFAATPLLLQMLPAPFQDWTTPL
                     LSDRYDSHLLPGGQKRGLLIGMGMTEKQGGSDVMSNTTRAERLDDGSYRLVGHKWFFS
                     VPQSDAHLVLAQTAGGLSCFFVPRFLPDGQRNAIRLERLKDKLGNRSNASCEVEFQDA
                     IGWLLGQEGEGTRLILKMGGMTRFDCALGSHAMMRRAFSLAIYHAHQRHVFGNPLIQQ
                     PLMRHVLSRMALQLEGQTALLFRLARAWDRCADAKEALWARLFTPAAKFVICKRGIPF
                     VAEAMEVLGGIGYCEESELPRLYREMPVNSIWEGSGNIMCLDVLRVLNKQAGVYDLLS
                     EAFVEVKGQDRYFDRAVRRLQQQLRKPAEELGREITHQLFLLGCGAQMLKYASPPMAQ
                     AWCKVMLDTRGGVRLSEQIQNDLLLRATGGVCL"
     gene            complement(5624..6787)
                     /locus_tag="J1Y83_000714"
     CDS             complement(5624..6787)
                     /locus_tag="J1Y83_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionylspermidine synthase family protein"
                     /protein_id="HBA7628896.1"
                     /translation="MLRHNVPVRRDLDRIAADNGFDFHIIDNEIYWDESRAYRFTLRQ
                     IEEQIEKPTAELHQMCLEVVDRAVKDEEILTQLAIPPLYWDVIAESWRARDPSLYGRM
                     DFAWCGNAPVKLLEYNADTPTSLYESAYFQWLWLEDARRSGVIPRDADQYNAIQERLI
                     SRFSELYSREPFYFCCCQDTDEDRSTVLYLQDCAQQAGQESRFIYIEDLGLGVGGVLT
                     DLDDNVIQRAFKLYPLEWMMRDDNGPLLRKRREQWVEPLWKSILSNKGLMPLLWRFFP
                     GHPNLLASWFDGEKPQIAAGESYVRKPIYSREGGNVTIFDGQNNVVDHADGDYADEPM
                     IYQAFQPLPRFGDSYTLIGSWIVDDEACGMGIREDNTLITKDTSRFVPHYIAG"
     gene            complement(6790..7428)
                     /locus_tag="J1Y83_000715"
     CDS             complement(6790..7428)
                     /locus_tag="J1Y83_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1190 domain-containing protein"
                     /protein_id="HBA7628897.1"
                     /translation="MARKRKSRNNKNIGHGAISRIGRPHNPFEPRRNRYAQKYLTLAL
                     MGGAAFFVLKGCGDSGDVDNDGDGTFYATVQDCIDDGNNSDICAHGWNNAKAAFYADV
                     PKNMTQQNCQSKYENCYYDNVEQSWIPVVSGFLLSRVIRKDRDEPFVYNSGGSSFASR
                     PVWRNTSGDYSWRSGSGKKESYSSGGFTTKKASTVSRGGYGRSSSARGHWGG"
     gene            complement(7438..7836)
                     /locus_tag="J1Y83_000716"
     CDS             complement(7438..7836)
                     /locus_tag="J1Y83_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF350 domain-containing protein"
                     /protein_id="HBA7628898.1"
                     /translation="MHILDSLLAFSAYFFIGVAMVIIFLFIYSKITPHNEWQLIKNNN
                     TAAALAFSGTLLGYVIPLSSAAINAVSIPDYFAWGGIALVIQLLVFAGVRLYMPALSE
                     KIINHNTAAGMFMGTAALAGGIFNAACMTW"
     gene            complement(7854..8513)
                     /locus_tag="J1Y83_000717"
     CDS             complement(7854..8513)
                     /locus_tag="J1Y83_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjfK family protein"
                     /protein_id="HBA7628899.1"
                     /translation="MSGFFQRLFGKDNKPAIARGPLGLHLNSGFTLDTLAFRLLEDAL
                     LIALPGEEYTVAAVSCIDLGGGSQIFRYYTSGDEFLQINTTGGEDIDDIDDIKLFVYE
                     ESYGISKESHWREAINAKAMGAMTLNWQEKRWQRFFNSEEPGNIEPVYMLEKVENQNH
                     AKWEVHNFTMGYQRQVTGDTYEYLLLNGEESFNDLGEPEWLFSRALGVDIPMTSLHII
                     G"
     gene            complement(8563..9261)
                     /locus_tag="J1Y83_000718"
     CDS             complement(8563..9261)
                     /locus_tag="J1Y83_000718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspA/IM30 family protein"
                     /protein_id="HBA7628900.1"
                     /translation="MGILKSLFTLGKSFISQAEESIEENQGVRMLEQHIRDAKTELDK
                     AGKSRVDLLARVKLSHDKLKDLRERKASLEARALEALSKNVNPSLINEVAEEIARLEN
                     LITAEEQVLSNLEVSRDGVEKAVTATAQRIAQFEQQMEVVKATEAMQRAQQAVTTSTV
                     GASSSVSTAAESLKRLQTRQAERQARLDAATQLEKVADGRDLDEKLAEAGIGGSNKSS
                     AQDVLARLQRQQGE"
     gene            complement(9280..9681)
                     /locus_tag="J1Y83_000719"
     CDS             complement(9280..9681)
                     /locus_tag="J1Y83_000719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2170 family protein"
                     /protein_id="HBA7628901.1"
                     /translation="MTWNPLALATALQTVPEQNIDVTNSESALIIKMNDYGDLQINIL
                     FTSRQMIIETFICPVSSISNPDEFNTFLLRNQKMMPLSSVGISSVQQEEYYIVFGALS
                     LKSSLEDILLEITSLVDNALDLAEITEEYSH"
     gene            complement(9808..10539)
                     /gene="rlmB"
                     /locus_tag="J1Y83_000720"
     CDS             complement(9808..10539)
                     /gene="rlmB"
                     /locus_tag="J1Y83_000720"
                     /EC_number="2.1.1.185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HBA7628902.1"
                     /translation="MSEMIYGIHAVQALLERAPERFQEVFILKGREDKRLLPLIHALE
                     SQGVVIQLANRQYLDEKSDGAVHQGIIARVKPGRQYQENDLPDLIASLDQPFLLILDG
                     VTDPHNLGACLRSADAAGVHAVIVPKDRSAQLNATAKKVACGAAESVPLIRVTNLART
                     MRMLQEENIWIVGTAGEADHTLYQSKMTGRLALVMGAEGEGMRRLTREHCDELISIPM
                     AGSVSSLNVSVATGICLFEAVRQRS"
     gene            complement(10630..13071)
                     /gene="rnr"
                     /locus_tag="J1Y83_000721"
     CDS             complement(10630..13071)
                     /gene="rnr"
                     /locus_tag="J1Y83_000721"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313182.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HBA7628903.1"
                     /translation="MSQDPFQEREAEKYANPIPSREFILEHLTKREKPASRDELAVEL
                     HIEGEEQLEGLRRRLRAMERDGQLVFTRRQCYALPERLDLVKGTVIGHRDGYGFLRVE
                     GRKDDLYLSSEQMKTCIHGDQVLAQPLGADRKGRREARIVRVLVPKTSQIVGRYFTEA
                     GVGFVVPDDSRLSFDILIPPDQIMGARMGFVVVVELTQRPTRRTKAVGKIVEVLGDNM
                     GTGMAVDIALRTHEIPYIWPQAVEQQVAGLKEEVPEEAKAGRVDLRDLPLVTIDGEDA
                     RDFDDAVYCEKKRGGGWRLWVAIADVSYYVRPPTPLDREARNRGTSVYFPSQVIPMLP
                     EVLSNGLCSLNPQVDRLCMVCEMTVSSKGRLTGYKFYEAVMSSHARLTYTKVWHILQG
                     DQDLREQYAPLVKHLEELHNLYKVLDKAREERGGISFESEEAKFIFNAERRIERIEQT
                     QRNDAHKLIEECMILANISAARFVEKAKEPALFRIHDKPSTEAITSFRSVLAELGLEL
                     PGGNKPEPRDYAELLESVADRPDAEMLQTMLLRSMKQAIYDPENRGHFGLALQSYAHF
                     TSPIRRYPDLTLHRAIKYLLAKEQGHQGNTTETGGYHYSMEEMLQLGQHCSMAERRAD
                     EATRDVSDWLKCDFMLDQVGNVFKGVISSVTGFGFFVRLDDLFIDGLVHVSSLDNDYY
                     RFDQVGQRLMGESSGQTYRLGDRVEVRVEAVNMDERKIDFSLISSERAPRNVGKTARE
                     KAKKGDAGKKGGKRRQVGKKVNFEPDSAFRGEKKPKPKAAKKDARKAKKPSAKTQKIA
                     AATKAKRAAKKKVAE"
     gene            complement(13110..13535)
                     /gene="nsrR"
                     /locus_tag="J1Y83_000722"
     CDS             complement(13110..13535)
                     /gene="nsrR"
                     /locus_tag="J1Y83_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide-sensing transcriptional repressor
                     NsrR"
                     /protein_id="HBA7628904.1"
                     /translation="MQLTSFTDYGLRALIYMASLPEGRMTSISEVTDVYGVSRNHMVK
                     IINQLSRAGYVTAVRGKNGGIRLGKPASAIRIGDVVRELEPLSLVNCSSEFCHITPAC
                     RLKQALSKAVQSFLTELDNYTLADLVEENQPLYKLLLVE"
     gene            complement(13740..15038)
                     /gene="purA"
                     /locus_tag="J1Y83_000723"
     CDS             complement(13740..15038)
                     /gene="purA"
                     /locus_tag="J1Y83_000723"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HBA7628905.1"
                     /translation="MGNNVVVLGTQWGDEGKGKIVDLLTERAKYVVRYQGGHNAGHTL
                     VINGEKTVLHLIPSGILRENVTSIIGNGVVLSPAALMKEMKELEDRGIPVRERLLLSE
                     ACPLILDYHVALDNAREKARGAKAIGTTGRGIGPAYEDKVARRGLRVGDLFDKETFAE
                     KLKEVMEYHNFQLVNYYKAEAVDYQKVLDDTMAVADILTSMVVDVSDLLDQARQRGDF
                     VMFEGAQGTLLDIDHGTYPYVTSSNTTAGGVATGSGLGPRYVDYVLGILKAYSTRVGA
                     GPFPTELFDETGEFLCKQGNEFGATTGRRRRTGWLDTVAVRRAVQLNSLSGFCLTKLD
                     VLDGLKEVKLCVAYRMPDGREVTTTPLAADDWKGVEPIYETMPGWSESTFGVKDRSGL
                     PQAALNYIKRIEELTGVPIDIISTGPDRTETMILRDPFDA"
     gene            complement(15142..15339)
                     /locus_tag="J1Y83_000724"
     CDS             complement(15142..15339)
                     /locus_tag="J1Y83_000724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2065 domain-containing protein"
                     /protein_id="HBA7628906.1"
                     /translation="MNSTIWLALALVLVLEGLGPMLYPKAWKKMISAMTNLPDNILRR
                     FGGGLVVAGVVVYYMLRKTIG"
     gene            complement(15421..16425)
                     /gene="hflC"
                     /locus_tag="J1Y83_000725"
     CDS             complement(15421..16425)
                     /gene="hflC"
                     /locus_tag="J1Y83_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease modulator HflC"
                     /protein_id="HBA7628907.1"
                     /translation="MRKSVIAIIIIVLVVLYMSVFVVKEGERGITLRFGKVLRDDDNK
                     PLVYEPGLHFKIPFIETVKMLDARIQTMDNQADRFVTKEKKDLIVDSYIKWRISDFSR
                     YYLATGGGDISQAEVLLKRKFSDRLRSEIGRLDVKDIVTDSRGRLTLEVRDALNSGSA
                     GTEDEVTTPAADNAIAEAAERVTAETKGKVPVINPNSMAALGIEVVDVRIKQINLPTE
                     VSEAIYNRMRAEREAVARRHRSQGQEEAEKLRATADYEVTRTLAEAERQGRIMRGEGD
                     AEAAKLFADAFSKDPDFYAFIRSLRAYENSFSGNQDVMVMSPDSDFFRYMKTPTSATR
                     "
     gene            complement(16428..17687)
                     /gene="hflK"
                     /locus_tag="J1Y83_000726"
     CDS             complement(16428..17687)
                     /gene="hflK"
                     /locus_tag="J1Y83_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease activity modulator HflK"
                     /protein_id="HBA7628908.1"
                     /translation="MAWNQPGNNGQDRDPWGSSKPGGNSEGNGNKGGRDQGPPDLDDI
                     FRKLSKKLGGLGGGKGTGSGGGSSSQGPRPQLGGRVVTIAAAAIVIIWAASGFYTIKE
                     AERGVVTRFGKFSHLVEPGLNWKPTFIDEVKPVNVEAVRELAASGVMLTSDENVVRVE
                     MNVQYRVTDPEKYLYSVTSPDDSLRQATDSALRGVIGKYTMDRILTEGRTVIRSDTQR
                     ELEETIRPYDMGITLLDVNFQAARPPEEVKAAFDDAIAARENEQQYIREAEAYTNEVQ
                     PRANGQAQRILEEARAYKAQTILEAQGEVARFAKLLPEYKAAPEITRERLYIETMEKV
                     LGNTRKVLVNDKGGNLMVLPLDQMLKGGNAPAAKSDNGASNLLRLPPASSSTTSGASN
                     TSSTSQGDIMDQRRANAQRNDYQRQGE"
     gene            complement(17773..19053)
                     /gene="hflX"
                     /locus_tag="J1Y83_000727"
     CDS             complement(17773..19053)
                     /gene="hflX"
                     /locus_tag="J1Y83_000727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HBA7628909.1"
                     /translation="MFDRYDAGEQAVLVHIYFTQDKDMEDLQEFESLVSSAGVEALQV
                     ITGSRKAPHPKYFVGEGKAVEIAEAVKATGASVVLFDHALSPAQERNLERLCECRVID
                     RTGLILDIFAQRARTHEGKLQVELAQLRHLATRLVRGWTHLERQKGGIGLRGPGETQL
                     ETDRRLLRNRIVQIQSRLERVEKQREQGRQSRIKADVPTVSLVGYTNAGKSTLFNRIT
                     EARVYAADQLFATLDPTLRRIDVADVGETVLADTVGFIRHLPHDLVAAFKATLQETRQ
                     ATLLLHVIDAADVRVQENIEAVNTVLEEIDAHEIPTLLVMNKIDMLEDFEPRIDRDEE
                     NKPIRVWLSAQTGAGIPQLFQALTERLSGEVAQHTLRLPPQEGRLRSRFYQLQAIEKE
                     WMEEDGSVSLQVRMPIVDWRRLCKQEPALIDYLI"
     gene            complement(19129..19437)
                     /gene="hfq"
                     /locus_tag="J1Y83_000728"
     CDS             complement(19129..19437)
                     /gene="hfq"
                     /locus_tag="J1Y83_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HBA7628910.1"
                     /translation="MAKGQSLQDPFLNALRRERVPVSIYLVNGIKLQGQIESFDQFVI
                     LLKNTVSQMVYKHAISTVVPSRPVSHHSNNAGGGTSSNYHHGSSAQNTSAQQDSEETE
                     "
     gene            complement(19523..20473)
                     /gene="miaA"
                     /locus_tag="J1Y83_000729"
     CDS             complement(19523..20473)
                     /gene="miaA"
                     /locus_tag="J1Y83_000729"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HBA7628911.1"
                     /translation="MSDISKASLPKAIFLMGPTASGKTALAIELRKILPVELISVDSA
                     LIYKGMDIGTAKPNAEELLAAPHRLLNIRDPSQAYSAADFRRDALAEMADITAAGRIP
                     LLVGGTMLYFKALLEGLSPLPSADPEVRARIEQQAAEQGWESLHRQLQEIDPVAAARI
                     HPNDPQRLSRALEVFFISGKTLTELTQTSGDALPYQVHQFAIAPASRELLHQRIEQRF
                     HQMLASGFEAEVRALFARGDLHTDLPSIRCVGYRQMWSYLEGEISYDEMVYRGVCATR
                     QLAKRQITWLRGWEGVHWLDSEKPEQARDEVLQVVGAIAG"
     gene            complement(20466..22313)
                     /gene="mutL"
                     /locus_tag="J1Y83_000730"
     CDS             complement(20466..22313)
                     /gene="mutL"
                     /locus_tag="J1Y83_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710035.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HBA7628912.1"
                     /translation="MPIQVLPPQLANQIAAGEVVERPASVVKELVENSLDAGATRIDI
                     DIERGGAKLIRIRDNGCGIKKDELALALARHATSKIASLDDLEAIISLGFRGEALASI
                     SSVSRLTLTSRTAEQQEAWQAYAEGRDMDVTVKPAAHPVGTTLEVLDLFYNTPARRKF
                     LRTEKTEFNHIDEIIRRIALARFDVTINLSHNGKIVRQYRAVPEGGQKERRLGAICGT
                     AFLEQALAIEWQHGDLTLRGWVADPNHTTPALAEIQYCYVNGRMMRDRLINHAIRQAC
                     EDKLGANQQPAFVLYLEIDPHQVDVNVHPAKHEVRFHQSRLVHDFIYQGVLSVLQQQL
                     ETPLPLDDEPQPAPRAIPENRVAAGRNHFAEPAVREPVAPRYSPAPASGSRPAASWPN
                     AQPGYQKQQGEVYRQLLQTPAPMQKPKAPEPQEPALAANSQSFGRVLTIVHSDCALLE
                     RDGNISLLSLPVAERWLRQAQLTPGEVPVCAQPLLIPLRLKVSGEEKSALEKAQSALA
                     ELGIDFQSDAQHVTIRAVPLPLRQQNLQILIPELIGYLAKQSVFEPGNIAQWIARNLM
                     SEHAQWSMAQAITLLADVERLCPQLVKTPPGGLLQSVDLHLAIKALKDE"
     gene            complement(22323..23660)
                     /gene="amiB"
                     /locus_tag="J1Y83_000731"
     CDS             complement(22323..23660)
                     /gene="amiB"
                     /locus_tag="J1Y83_000731"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiB"
                     /protein_id="HBA7628913.1"
                     /translation="MMYRIRNWLVATLLLLCAPVGAATLSDIQVSNGNQQARITLSFI
                     GDPDYAFSHQSKRTVALDIKQTGVIQGLPLLFSGNNLVKAIRSGTPKDAQTLRLVVDL
                     TENGKTEAVKRQNGSNYTVVFTINADAPPPPPPPPVVAKRVETPAVVAPRVSEPARNP
                     FKTESNRTTGVISSNTVTRPAARATANTGDKIIIAIDAGHGGQDPGAIGPGGTREKNV
                     TIAIARKLRTLLNDDPMFKGVLTRDGDYFISVMGRSDVARKQNANFLVSIHADAAPNR
                     SATGASVWVLSNRRANSEMASWLEQHEKQSELLGGAGDVLANSQADPYLSQAVLDLQF
                     GHSQRVGYDVATSMISQLQRIGEIHKRRPEHASLGVLRSPDIPSVLVETGFISNNSEE
                     RLLASDDYQQQLAEAIYKGLRNYFLAHPMQSAPQGATAQTASTVTTPDRTLPN"
     gene            complement(23679..24140)
                     /gene="tsaE"
                     /locus_tag="J1Y83_000732"
     CDS             complement(23679..24140)
                     /gene="tsaE"
                     /locus_tag="J1Y83_000732"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HBA7628914.1"
                     /translation="MMNRVIPLPDEQATLDLGERVAKACDGATVIYLYGDLGAGKTTF
                     SRGFLQALGHQGNVKSPTYTLVEPYTLDNLMVYHFDLYRLAEPEELEFMGIRDYFAND
                     AICLVEWPQQGTGVLPDPDVEIHIDYQAQGREARVSAVSSAGELLLARLAG"
     gene            complement(24112..25659)
                     /gene="nnr"
                     /locus_tag="J1Y83_000733"
     CDS             complement(24112..25659)
                     /gene="nnr"
                     /locus_tag="J1Y83_000733"
                     /EC_number="4.2.1.136"
                     /EC_number="5.1.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional ADP-dependent NAD(P)H-hydrate
                     dehydratase/NAD(P)H-hydrate epimerase"
                     /protein_id="HBA7628915.1"
                     /translation="MTDHTMKKNPVSIPHTVWYADHIRRGEREAADALGLTLYELMLR
                     AGEAAFQVCRSAYPDARHWLVLCGHGNNGGDGYVVARLAKAIGIDVTLLAQESDKPLP
                     EEAALAREAWLNAGGEIHASNIVWPESVDLIVDALLGTGLQQAPRESISQLIDHANTH
                     PAPIVAVDIPSGLLAETGATPGAVINADHTITFIALKPGLLTGKARDVTGQLHFDSLG
                     LDSWLAGQETKIQRFSAEQLSQWLIPRRPTSHKGDHGRLVIIGGDHGTAGAIRMTGEA
                     ALRAGAGLVRVLTRSENIAPLLTARPELMVHELTMDSLTESLEWADVVVIGPGLGQQE
                     WGKKALQKVENFRKPMLWDADALNLLAINPDKRHNRVITPHPGEAARLLGCSVAEIES
                     DRLHCTQRLVQRYGGVAVLKGAGTVVAAHPDALGIIDAGNAGMASGGMGDVLSGIIGA
                     LLGQKLSPYDAACAGCVAHGAAADVLAARFGTRGMLATDLFSTLQRIVNPEVTDKNHD
                     ESSNSAP"
     gene            25658..26797
                     /gene="queG"
                     /locus_tag="J1Y83_000734"
     CDS             25658..26797
                     /gene="queG"
                     /locus_tag="J1Y83_000734"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HBA7628916.1"
                     /translation="MSEPLDLNQLAQNIKQWGLELGFQQVGITDTDLSASEPKLQAWL
                     DKQYHGEMDWMARHGMLRARPHELLPGTLRVISVRMNYLPANAAFASTLKKTKLGYVS
                     RYALGRDYHKLLRNRLKKLGEMIQQHCVSLNFRPFVDSAPILERPLAEKAGLGWTGKH
                     SLILNREAGSFFFLGELLVDIPLPVDQPVEEGCGKCVACMTICPTGAIVEPYTVDARR
                     CISYLTIELEGAIPEELRPLMGNRIYGCDDCQLICPWNRYSQLTTEDDFSPRKPLHAP
                     ELIELFAWSEEQFLKVTEGSAIRRIGHLRWLRNIAVALGNAPWDETILTALESRKGEH
                     PLLDEHIAWAIAQQIERRNACVVEVQLPKKQRLVRVIEKGLPRDA"
     gene            complement(26780..26833)
                     /locus_tag="J1Y83_000735"
     CDS             complement(26780..26833)
                     /locus_tag="J1Y83_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA7628917.1"
                     /translation="MRFYFYSQAVDESGVTR"
     gene            complement(27067..27142)
                     /locus_tag="J1Y83_000736"
     tRNA            complement(27067..27142)
                     /locus_tag="J1Y83_000736"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(27107..27109),aa:Gly,seq:gcc)
     gene            complement(27353..27898)
                     /gene="orn"
                     /locus_tag="J1Y83_000737"
     CDS             complement(27353..27898)
                     /gene="orn"
                     /locus_tag="J1Y83_000737"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418586.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoribonuclease"
                     /protein_id="HBA7628918.1"
                     /translation="MSANENNLIWIDLEMTGLDPERDRIIEIATLVTDANLNILAEGP
                     TIAVHQSDEQLALMDDWNVRTHTASGLVERVKASTMGDREAELATLEFLKQWVPAGKS
                     PICGNSIGQDRRFLFKYMPELEAYFHYRYLDVSTLKELARRWKPEILDGFTKQGTHQA
                     MDDIRESVAELAYYREHFIKL"
     gene            27993..29045
                     /gene="rsgA"
                     /locus_tag="J1Y83_000738"
     CDS             27993..29045
                     /gene="rsgA"
                     /locus_tag="J1Y83_000738"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313167.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small ribosomal subunit biogenesis GTPase RsgA"
                     /protein_id="HBA7628919.1"
                     /translation="MSKNKLSKGQQRRVNANHQRRLKTSKEKPDYDDNLFGEPDEGIV
                     ISRFGMHADVESADGDVHRCNIRRTIRSLVTGDRVVWRPGKPAAEGVNVKGIVEAVHE
                     RTSVLTRPDFYDGVKPIAANIDQIVIVSAILPELSLNIIDRYLVACETLQIEPIIVLN
                     KIDLLDDEGMEFVNEQMDIYRNIGYRVLMVSSHTQDGLKPLEEALTGRISIFAGQSGV
                     GKSSLLNALLGLQKEILTNDVSDNSGLGQHTTTAARLYHFPHGGDVIDSPGVREFGLW
                     HLEPEQITQGFVEFHDYLGLCKYRDCKHDTDPGCAIREAVEEGKIAETRFENYHRILE
                     SMAQVKTRKNFSDTDD"
     gene            29143..30111
                     /gene="psd"
                     /locus_tag="J1Y83_000739"
     CDS             29143..30111
                     /gene="psd"
                     /locus_tag="J1Y83_000739"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313166.1"
                     /note="Phosphatidylserine decarboxylase is synthesized as
                     a single chain precursor. Generation of the pyruvoyl
                     active site from a Ser is coupled to cleavage of a Gly-Ser
                     bond between the larger (beta) and smaller (alpha chains).
                     It is an integral membrane protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase"
                     /protein_id="HBA7628920.1"
                     /translation="MLNSFKLSLQYILPKLWLTRLAGWGASKRAGWLTKLVIDLFVKY
                     YKVDMKEAQKPDTASYRTFNEFFVRPLRDEVRPIDTDPNVLVMPADGVISQLGKIEED
                     KILQAKGHNYSLEALLAGNYLMADLFRNGTFVTTYLSPRDYHRVHMPCNGILREMIYV
                     PGDLFSVNHLTAQNVPNLFARNERVICLFDTEFGPMAQILVGATIVGSIETVWAGTIT
                     PPREGIIKRWTWPAGENDGSVALLKGQEMGRFKLGSTVINLFAPGKVNLVEQLESLSV
                     TKIGQPLAVSTETFVTPDAEPAPLPAEEIEAEHDASPLVDDKKDQV"
     gene            30133..33456
                     /gene="mscM"
                     /locus_tag="J1Y83_000740"
     CDS             30133..33456
                     /gene="mscM"
                     /locus_tag="J1Y83_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="miniconductance mechanosensitive channel MscM"
                     /protein_id="HBA7628921.1"
                     /translation="MRLIITFLMAWCLSWGAYAATAPDSKQITQELEQAKAAKPAQPE
                     VVEALQSALNALEERKGSLERIKQYQEVIDNYPKLSATLRAQLNNMRDEPRSVSPGMS
                     NDALNQEILQISSQLLDKSRQAQQEQERAREIADSLNQLPQQQTDARRQLNEIERRLG
                     TLTGNTPLNQAQNFALQSDSARLKALVDELELAQLSANNRQELARLRSELAEKESQQL
                     DAYLQALRNQLNSQRQLEAERALESTEQLAESSADLPKDIVAQFKINRELSAALNQQA
                     QRMDLVASQQRQAASQTLQVRQALNTLREQSQWLGSSNLLGEALRAQVARLPEMPKPQ
                     QLDTEMAQLRVQRLRYEDLLNKQPLLRQIHQADGQPLTAEQNRILEAQLRTQRELLNS
                     LLQGGDTLLLELTKLKVSNGQLEDALKEVNEATHRYLFWTSDVRPMTIAWPLEIAQDL
                     RRLISLDTFSQLGKASVMMLTSKETILPLFGALILVGCSIYSRRYFTRFLERSAAKVG
                     KVTQDHFWLTLRTLFWSILVASPLPVLWMTLGYGLREAWPYPLAVAIGDGVTATVPLL
                     WVVMICATFARPNGLFIAHFGWPRERVSRGMRYYLMSIGLIVPLIMALMMFDNLDDRE
                     FSGSLGRLCFILICGALAVVTLSLKKAGIPLYLNKEGSGDNITNHMLWNMMIGAPLVA
                     ILASAVGYLATAQALLARLETSVAIWFLLLVVYHVIRRWMLIQRRRLAFDRAKHRRAE
                     MLAQRARGEEEAHHHSSPEGAIEVDESEVDLDAISAQSLRLVRSILMLIALLSVIVLW
                     SEIHSAFGFLENISLWDVTSTVQGVESLEPITLGAVLIAILVFIITTQLVRNLPALLE
                     LAILQHLDLTPGTGYAITTITKYLLMLIGGLVGFSMIGIEWSKLQWLVAALGVGLGFG
                     LQEIFANFISGLIILFEKPIRIGDTVTIRDLTGSVTKINTRATTISDWDRKEIIVPNK
                     AFITEQFINWSLSDSVTRVVLTIPAPADANSEEVTEILLTAARRCSLVIDNPAPEVFL
                     VDLQQGIQIFELRIYAAEMGHRMPLRHEIHQLILAGFHAHGIDMPFPPFQMRLESLNG
                     KQTGRTLTSAGKGRQAGSL"
     gene            complement(33487..33801)
                     /locus_tag="J1Y83_000741"
     CDS             complement(33487..33801)
                     /locus_tag="J1Y83_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjeO family protein"
                     /protein_id="HBA7628922.1"
                     /translation="MSARMFILCCIWFVVAIPWILITSTLDKEWMIDEGGVKNICDVL
                     EYLENDDTRDVGVILTLPLFFPFFWFALWRKKRGWFMYATTLAIFGHWLWQFFLRYHF
                     CL"
     gene            complement(33798..34120)
                     /locus_tag="J1Y83_000742"
                     /pseudo
     CDS             complement(33798..34120)
                     /locus_tag="J1Y83_000742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000340721.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(34183..35685)
                     /gene="yjeM"
                     /locus_tag="J1Y83_000743"
     CDS             complement(34183..35685)
                     /gene="yjeM"
                     /locus_tag="J1Y83_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418580.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/gamma-aminobutyrate family transporter
                     YjeM"
                     /protein_id="HBA7628923.1"
                     /translation="MPHTIKKMSLIGLILMIFTSVFGFANSPSAYYLMGYSAIPFYIF
                     SALLFFIPFALMMAEMGAAYRKEEDGIYSWMNNSVGPRFAFIGTFMWFSSYIIWMVST
                     SAKVWVPFSTFLYGSDMTQHWHIAGLEPTQVVGLLAVAWMILVTVVASKGINKIARIT
                     AVGGIAVMCLNLVLLLVSITILLLNGGHFAQDINFLASPNPGYQSGLAMLSFVVFAIF
                     AYGGIEAVGGLVDKTENPEKNFAKGIVFAAIVISIGYSLAIFLWGVSTNWQQVLSNGS
                     VNLGNITYVLMKSLGVTLGNALHLSPEASLSLGVWFARITGLSMFLAYTGAFFTLCYS
                     PLKAIIQGTPKALWPEPMTRLNAMGMPSIAMWMQCGLVTVFILLVSFGGGTASAFFNK
                     LTLMANVSMTLPYLFLALAFPFFKARQDLDRPFVIFKTHMSAMIATVVVVLVVAFANV
                     FTIIQPVVEAGDWDSTLWMIGGPVFFSLLAMAIYQNYCSRMANKPELALD"
     gene            complement(35904..36881)
                     /gene="epmA"
                     /locus_tag="J1Y83_000744"
     CDS             complement(35904..36881)
                     /gene="epmA"
                     /locus_tag="J1Y83_000744"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000004771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P--(R)-beta-lysine ligase"
                     /protein_id="HBA7628924.1"
                     /translation="MSETASWQPSASIPNLLKRAAIMAEIRRFFADRGVLEVETPCMS
                     QATVTDIHLVPFETRFVGPGHSQGMNLWLMTSPEYHMKRLLVAGCGPVFQLCRSFRNE
                     EMGRYHNPEFTMLEWYRPHYDMYRLMNEVDDLLQQVLDCPAAESLSYQQAFLRYLEID
                     PLSADKTQLREVAAKLDLSNVADTEEDRDTLLQLLFTFGVEPNIGKEKPTFVYHFPAS
                     QASLAQISTEDHRVAERFEVYYKGIELANGFHELTDAREQQQRFEQDNRKRAARGLPQ
                     HPIDQNLIEALKVGMPDCSGVALGVDRLVMLALGAETLAEVIAFSVDRA"
     gene            37206..39014
                     /gene="frdA"
                     /locus_tag="J1Y83_000745"
     CDS             37206..39014
                     /gene="frdA"
                     /locus_tag="J1Y83_000745"
                     /EC_number="1.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase (quinol) flavoprotein
                     subunit"
                     /protein_id="HBA7628925.1"
                     /translation="MQTFQADLAIVGAGGAGLRAAIAAAQANPNAKIALISKVYPMRS
                     HTVAAEGGSAAVAQDHDSFEYHFHDTVAGGDWLCEQDVVDYFVHHCPTEMTQLELWGC
                     PWSRRPDGSVNVRRFGGMKIERTWFAADKTGFHMLHTLFQTSLQFPQIQRFDEHFVLD
                     ILVDDGHVRGLVAMNMMEGTLVQIRANAVVMATGGAGRVYRYNTNGGIVTGDGMGMAL
                     SHGVPLRDMEFVQYHPTGLPGSGILMTEGCRGEGGILVNKNGYRYLQDYGMGPETPLG
                     EPKNKYMELGPRDKVSQAFWHEWRKGNTISTPRGDVVYLDLRHLGEKKLHERLPFICE
                     LAKAYVGVDPVKEPIPVRPTAHYTMGGIETDQNCETRIKGLFAVGECSSVGLHGANRL
                     GSNSLAELVVFGRLAGEQATERAATAGNGNEAAIEAQAAGVEQRLKDLVNQDGGENWA
                     KIRDEMGMAMEEGCGIYRTPELMQKTIDKLAELQERFKRVRITDTSSVFNTDLLYTIE
                     LGHGLNVAECMAHSAMARKESRGAHQRLDEGCTERDDVNFLKHTLAFRDADGTTRLEY
                     SDVKITTLPPAKRVYGGEADAADKAEAANKKEKANG"
     gene            39007..39741
                     /locus_tag="J1Y83_000746"
     CDS             39007..39741
                     /locus_tag="J1Y83_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase/fumarate reductase
                     iron-sulfur subunit"
                     /protein_id="HBA7628926.1"
                     /translation="MAEMKNLKIEVVRYNPEVDTAPHSAFYEVPYDATTSLLDALGYI
                     KDNLAPDLSYRWSCRMAICGSCGMMVNNVPKLACKTFLRDYTDGMKVEALANFPIERD
                     LVVDMTNFIESLEAIKPYIIGNSRTADQGTNIQTPAQMAKYHQFSGCINCGLCYAACP
                     QFGLNPEFIGPAAITLAHRYNEDSRDHGKKERMAQLNSQNGVWSCTFVGYCSEVCPKH
                     VDPAAAIQQGKVESSKDFLIATLKPR"
     gene            39752..40147
                     /gene="frdC"
                     /locus_tag="J1Y83_000747"
     CDS             39752..40147
                     /gene="frdC"
                     /locus_tag="J1Y83_000747"
                     /EC_number="1.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdC"
                     /protein_id="HBA7628927.1"
                     /translation="MTTKRKPYVRPMTSTWWKKLPFYRFYMLREGTAVPAVWFSIELI
                     FGLFALKNGPEAWAGFVDFLQNPVIVIINLITLAAALLHTKTWFELAPKAANIIVKDE
                     KMGPEPIIKSLWAVTVVATIVILFVALYW"
     gene            40158..40517
                     /gene="frdD"
                     /locus_tag="J1Y83_000748"
     CDS             40158..40517
                     /gene="frdD"
                     /locus_tag="J1Y83_000748"
                     /EC_number="1.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdD"
                     /protein_id="HBA7628928.1"
                     /translation="MINPNPKRSDEPVFWGLFGAGGMWSAIIAPVMILLVGILLPLGL
                     FPGDALSYERVLAFAQSFIGRVFLFLMIVLPLWCGLHRMHHAMHDLKIHVPAGKWVFY
                     GLAAILTVVTLIGVVTI"
     gene            40580..41713
                     /locus_tag="J1Y83_000749"
     CDS             40580..41713
                     /locus_tag="J1Y83_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cephalosporin-hydrolyzing class C beta-lactamase
                     EC-5"
                     /protein_id="HBA7628929.1"
                     /translation="MFKTTLCTLLITASCSTFAAPQQINDIVHRTITPLIEQQKIPGM
                     AVAVIYQGKPYYFTWGYADIAKKQPVTQQTLFELGSVSKTFTGVLGGDAIARGEIKLS
                     DPTTKYWPELTAKQWNGITLLHLATYTAGGLPLQVPDEVKSSSDLLRFYQNWQPAWAP
                     GTQRLYANSSIGLFGALAVKPSGLSFEQAMKTRVFQPLKLNHTWINVPSAEEKNYAWG
                     YREGKAVHVSPGALDAEAYGVKSTIEDMARWVQSNLKPLDINEKTLQQGIQLAQSRYW
                     QTGDMYQGLGWEMLDWPVNPDIIINGSDNKIALAARPVKPITPPTPAVRASWVHKTGA
                     TGGFGSYVAFIPEKELGIVMLANKNYPNPARVAAAWQILNALQ"
     gene            41802..42335
                     /locus_tag="J1Y83_000750"
     CDS             41802..42335
                     /locus_tag="J1Y83_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin family protein"
                     /protein_id="HBA7628930.1"
                     /translation="MRLLPLVAAATAAFLVVACSSPTPPRGVTVVNNFDAKRYLGTWY
                     EIARFDHRFERGLEKVTATYSLRDDGGLNVINKGYNPDREMWQQSEGKAYFTGVPTRA
                     ALKVSFFGPFYGGYNVIALDREYRHALVCGPDRDYLWILSRTPTISDEVKQEMLAVAT
                     REGFDVSKFIWVQQPGS"
     gene            complement(42332..42649)
                     /locus_tag="J1Y83_000751"
     CDS             complement(42332..42649)
                     /locus_tag="J1Y83_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418572.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound-resistance protein
                     SugE"
                     /protein_id="HBA7628931.1"
                     /translation="MSWIILVIAGLLEVVWAVGLKYTHGFSRLTPSVITVTAMIVSLA
                     LLAWAMKSLPVGTAYAVWTGIGAVGAAITGIVLLGESANPMRLASLALIVLGIIGLKL
                     STH"
     gene            complement(42825..>42970)
                     /gene="ecnB"
                     /locus_tag="J1Y83_000752"
     CDS             complement(42825..>42970)
                     /gene="ecnB"
                     /locus_tag="J1Y83_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lipoprotein toxin entericidin B"
                     /protein_id="HBA7628932.1"
                     /translation="VKKTIAAIFSVLVLSTVLTACNTTRGVGEDISDGGNAISGAATK
                     AQQ"
BASE COUNT        10482 a        11709 c        10720 g        10059 t
ORIGIN      
        1 tattcattta aattcattta gttaattgca ttacacattc aatttaatct ttccacactc
       61 aaaatccaca ataactctcc tgctaaaaag tcaaaaaatg gtcattgcct tcctgaaaaa
      121 atagtgtcag gattaatcaa aaccaatcac atgttgattc gaattgaact atcacgaagg
      181 taaatggcga tgagtaaagt gaaaagtatc acccgtgagt cctggatcct gagcactttc
      241 ccggagtggg gtagctggct gaatgaagaa attgagcaag agcaggtcgc acctggcact
      301 ttcgcaatgt ggtggctcgg ctgtaccggg atctggttga aatcagaagg cggtgccaac
      361 gtttgtgttg atttttggtg cggtactggc aaacaaagcc acggtaaccc gttcatgaaa
      421 cagggtcacc agatgcagcg catggctggc gtgaaaaaac tgcagccaaa cctgcgcact
      481 accccgtttg ttcttgatcc gtttgcgatt cgccagatcg acgcggtact ggcgactcac
      541 gatcacaacg atcatatcga cgttaacgtc gctgctgccg tgatgcagaa ctgtgctgat
      601 gacgtaccgt tcattggacc gaaaacctgc gttgatttat ggattggctg gggcgtaccg
      661 aaagagcgtt gcatcgtcgt taaaccgggc gacatagtga aggtaaaaga cattgaaatt
      721 cattcacttg atgctttcga ccgcacggca ctgatcaccc tacctgccga tcaaaaagca
      781 gctggcgtac tgccggacgg catggacgat cgcgcggtga actatctgtt caaaacccca
      841 ggtggtaccc tgtatcacag cggcgattcc cactactcta actactatgc aaaacatggt
      901 aatgagcatc agatcgacgt ggctttaggt tcatacggcg aaaacccacg cggtattacc
      961 gacaaaatga ccagtgctga tatcttgcgt atgggcgaag cgctgaatgc gaaagtagtg
     1021 atcccgttcc accacgatat ctggtcaaac ttccaggccg atccgcaaga gatccgcgtg
     1081 ctgtgggaga tgaaaaaaga tcgcctgaag tatggcttca agccgtttat ctggcaggtc
     1141 ggcggcaaat ttacctggcc gctggataaa gacaacttcg agtaccacta tccgcgtggt
     1201 ttcgatgatt gcttcactat tgaaccagat ctgccgttca agtcgttcct gtaatctgtc
     1261 gcttgtaccg gatagcgtgg agatgctcgc gctatccggc atagtatttc ctggctattt
     1321 caaatatcat ctaaaaaaat caaattttat cggaatagct catgactgaa gcacaaagac
     1381 atcaaatcct cctggaaatg ctcgcacaat tgggctttgt gaccgttgag aaagtcgttg
     1441 agcgtctggg aatttcgcct gccactgcac gacgcgatat caataaactt gacgaaagcg
     1501 gcaaactgaa aaaagtgcgc aatggcgcag aagctattac ccaacagcgc ccgcgctgga
     1561 cgccgatgaa tctgcatcag gcgcagaatc acgatgaaaa agtacgtatt gctaaagcgg
     1621 cctcgcagct ggttaatccg ggcgaaagcg tagtcatcaa ctgcggctcc accgcgtttc
     1681 tgcttgggcg ggaaatgtgt ggcaagccag tgcaaatcat cactaattat ctaccgctgg
     1741 caaactacct gatcgatcag gaacatgaca gcgtgatcat tatgggcgga cagtacaaca
     1801 aaagccagtc catcacctta agcccgcagg gcagcgaaaa cagtctctat gccgggcact
     1861 ggatgtttac cagcggcaaa ggactgacag cagaagggtt gtataaaacc gatatgctga
     1921 cggcaatggc agagcagaag atgctgagcg tggtagggaa actggtggta ctggttgata
     1981 gcagtaagat tggcgaacgc gcgggaatgc tttttagccg tgccgatcaa atcgatatgc
     2041 tgatcaccgg caaaaatgct aacccggaaa tcctgcaaca gctggaagcg caaggggtca
     2101 gcattatgcg tgtttaaaga tgctggcgga aaaatgtcac ggcagcatct aacgcctcag
     2161 gcgtaatgcg gtgacgcacg cctggctgcc atgaacaggt tagcagttta tcccgccccg
     2221 tctcgcttaa ggcctgctgc aaacgtagtg attcgtcggc aggcacgacg tcatcgtcaa
     2281 ggccatgcca cagcagtaga ggtctgtcac caagttgttc caggtggttt gtcgcttccc
     2341 actctgccag cggcgcgaca atgttattaa attcattctg ctgtgctgcc gtttcaggta
     2401 tcagcggtgg aaacagtgaa cgggcgagcg atgtaaaata gcccgatccc atcatgctgg
     2461 cggtacattt taccgtgggg tggcgagcgg taattcccag tgccgtcatc gcgcccatcg
     2521 acgcgccacc gactgccaga cggtcatcaa gcagccagtt ttcttcggct attgccgcac
     2581 gtaaggtagt gaattcctgc atactttgta gcaagatttg ccagaattga tttaaccgcc
     2641 gcgctgcgtc gccactaaaa cggctaccgt gatcgggcgc atccggcatg atcacccgca
     2701 aaccagcctg cgccagcgca acggcaaaat agctatacac cagactggac gaagtaaagc
     2761 cgtgataaaa aatcacacac ggtaacgggg tatctttttg cccgacagga taagcatgaa
     2821 gaacgggaat atctgccagc tcgcgtgatt ctatttcaat catctgtctc tcctttttgt
     2881 ctggtatgac atgaagaaat ccttagcaca agtttctgct ggcggtatta gttcataatg
     2941 ttgagatgtg ggttacgctt tcgtcacgtt ttcattcgaa aatcgcgtac caggtaacaa
     3001 ttcgggaaca ttcccccaaa gttaaattaa taaggcacta cactatggtt agcaggaaac
     3061 gtaatagcgt tatttaccgg tttgccagtt tattattggt gctgatgtta agtgcctgta
     3121 gcgcactgca aggtacgcca cagccagcac cgccagtaac ggaccatccg caagagattc
     3181 gccgcgacca gacgcaaggg ttacagcgaa taggtagcgt aagcacgatg gttcggggtt
     3241 ctccggatga cgcattagca gaaatcagag cgaaagctgt cgctgcaaaa gctgattatt
     3301 acgttgtcgt catggtggac gaaaccatcg tgacaggaca gtggtattca caagccattt
     3361 tgtaccgtaa ataaatcaga caacttacat agtcagcttt acattgcttt gcgtccctat
     3421 gcgtttacct gtgcacaata aattaccggc tggaagctcg ggcgtttatt accgcgatgg
     3481 aatgccctgc aacatgtggg gaaacgaaaa atggagctga cgatgaaaca attacttgcc
     3541 tcaccctcgc tgcaattagt gacttatcct gcgagcgcca cggcacagtc tgcagaattc
     3601 gccagtgctg actgcgtgac gggcctgaat gaaataggtc agatctcagt tagcaatatt
     3661 tcaggggatc cgcaggacgt ggaacgtatc gtggcgttaa aggccgatga acaaggtgcg
     3721 tcatggtacc gcattattac catgtacgaa gaccagcagc ctgataactg gcgcgtacag
     3781 gcgatactgt atgcgtgagt gtcatctaca gcaaatgaac aatgccggat gcggcgcgag
     3841 cgccttgtcc ggcctacaat tttaaaatat ttcatttagt taatcgaaac cagcgtcgca
     3901 tcagtcgtat acgcttacaa acacactcct cccgtcgccc gcagcaataa atcattctgg
     3961 atctgctctg acaaccgtac gccgccgcgc gtatctaaca tcaccttaca ccacgcctgc
     4021 gccattggcg gagaggcata tttcaacatt tgcgcaccgc agcccagcag gaatagctga
     4081 tgagtaatct ctcgccccaa ttcttcagct ggcttacgca gctgctgctg taaacgacga
     4141 accgcgcgat caaaatagcg atcctgccct ttcacttcca caaatgcttc cgacaataag
     4201 tcgtatacgc ccgcttgctt attgagaacg cgcagcacat ccaggcacat aatattgccg
     4261 gaaccttccc aaatactgtt taccggcatc tcccggtaaa gccgcggcag ctcgctctcc
     4321 tcgcaataac caatgccgcc cagcacctcc atcgcttcgg ccacaaacgg aatgccacgt
     4381 ttgcagatca caaatttcgc cgcaggcgta aataaacgcg cccacagggc ttctttggca
     4441 tcggcacacc ggtcccacgc tcgcgcaaga cgaaacagca acgccgtttg cccttcaagc
     4501 tgaagtgcca tgcgacttaa gacatgacgc ataagaggct gttggatcaa tggattacca
     4561 aaaacatggc gttgatgtgc atgataaatc gccagcgaaa atgcacggcg catcatggca
     4621 tggctaccca gggcgcaatc aaaacgcgtc atccctccca ttttcaggat cagacgagtt
     4681 ccttcccctt cctgccctaa caaccagcca atggcatcct gaaactccac ttcgcaactg
     4741 gcgttagagc gattacccag cttatctttc agccgctcga ggcgaatcgc gttgcgttgc
     4801 ccgtcaggca aaaagcgcgg cacaaaaaag caggacaaac cgcccgcggt ctgtgccagc
     4861 accagatgcg catcgctttg cggcaccgag aaaaaccatt tatgccccac cagccgataa
     4921 gaaccatcgt ccagacgctc tgcgcgggtg gtgttgctca tgacatcgga accgccctgc
     4981 ttttccgtca ttcccatgcc gatcaacaag ccacgttttt gcccacctgg caataagtga
     5041 gaatcgtagc gatcgctcag cagcggcgtg gtccagtcct gaaacggcgc gggtaacatc
     5101 tgtaacaaca atggcgtggc ggcaaaggtc atcgttatcg gacataacga ccctgcctca
     5161 acctgcgcat gtaacataaa acgcgctgcg cgcgccacaa atgtgccgga gcgagcgtct
     5221 tcttcccagg caagattgtg cacccgattg gtacatagcg cctgcatcag caggtgccag
     5281 gcggggtgaa aatgcacatc gtccagacgg cgtccttgcg catcgtagcg caataattca
     5341 ggcggattca cattcgccag ccgccccagt tcaagggatt cagccgttcc taactgctga
     5401 ccaatactgg cgagaaaatc gctatcccag ccagcacctt cacgcgttac cgcttcgcaa
     5461 agcgcgccat cagacaggta taagttgctg ttatttaatg gtataggttg attaaaaacg
     5521 gtgtgagttt gccagtgcac tgtgtctccc tccatcaatg gcagtcacca ttagtatggt
     5581 cactgccatg gattcatgac agaaagagat tgctaaaaca ttcttatcca gcaatgtaat
     5641 gcggaacgaa acgtgaggtg tctttggtga tcagcgtgtt atcttcacgg atccccattc
     5701 cgcacgcttc atcatcgaca atccagctac cgatgagtgt gtagctatcg ccaaatcgcg
     5761 gcagaggttg aaacgcctgg tagatcatcg gttcatcggc gtaatcacca tcagcgtggt
     5821 caacaacgtt attctgaccg tcgaaaatgg tgacgtttcc gccttcgcgc gagtaaatcg
     5881 gtttacgcac atagctttcg ccagcggcaa tctgcggttt ttcaccatcg aaccaggatg
     5941 caagaaggtt aggatgacct gggaagaagc gccaaagcag cggcattagc cctttattac
     6001 tcaagatact tttccataac ggctccaccc attgctcgcg acgcttgcgc agcagcggac
     6061 cgttatcgtc acgcatcatc cactccagcg gatacagctt aaatgcacgc tggatgacat
     6121 tatcatcaag atcggtcagt acgccgccga cgcccaaacc gagatcttca atgtagataa
     6181 accgcgactc ctgccctgcc tgctgggcgc agtcctgcaa gtacagcacg gtactcctgt
     6241 cttcatcggt gtcctgacag cagcaaaaat aaaacggttc ccgactgtaa agctcgctaa
     6301 agcgtgaaat caggcgttcc tgaatggcat tgtactgatc ggcatcacgc ggaataacgc
     6361 cgctgcgccg ggcatcttcc agccacaacc actggaaata agccgactcg tacaatgaag
     6421 ttggcgtatc ggcgttgtac tccagcaact taaccggtgc attaccacac caggcaaaat
     6481 ccatacggcc atacaacgaa ggatcgcgtg cgcgccagct ttcggcgatc acatcccagt
     6541 acaacggcgg gatcgccagt tgcgtcagga tctcttcatc tttaaccgca cgatccacca
     6601 cctccaggca catctgatgc agttccgcag tcggtttttc gatctgctct tcaatctggc
     6661 gcaaagtaaa gcggtaagcc cgactctcat cccaatagat ttcattgtcg atgatatgaa
     6721 agtcaaatcc gttatcggcg gcaatccggt ccagatcccg ccgcacagga acgttgtgtc
     6781 tcagcatgat tagcctcccc aatgcccacg ggcgctggaa gaacgaccat agccgccgcg
     6841 agaaacggtt gacgcttttt tggtggtaaa gccgcccgaa gagtaagact cttttttgcc
     6901 agaaccggag cgccaggagt aatcaccgga agtgttgcgc cagacggggc gggaagcaaa
     6961 tgaggatccg ccactgttat agacgaacgg ctcatcgcga tctttacgaa taacccggct
     7021 taacaaaaat ccagaaacga ccgggatcca actctgttca acattgtcgt agtagcaatt
     7081 ttcgtactta gactgacaat tctgctgagt catattcttc ggaacatcgg cataaaatgc
     7141 cgctttggcg ttgttccagc catgagcaca gatatctgaa ttattaccat catcaataca
     7201 atcctgcaca gtcgcgtaaa acgttccgtc gccgtcgtta tcgacatcgc cactatcgcc
     7261 acaacctttc aatacgaaaa aagcggcacc gcccattagt gccaacgtta agtatttttg
     7321 tgcgtagcga ttgcggcgcg gctcaaaagg attatgcggt ctaccaatgc gactgattgc
     7381 cccatgtccg atatttttgt tatttctgga tttgcgtttt ctggccatga tcgtccatta
     7441 ccatgtcata caagctgcgt taaaaatacc gccagccagc gcggcggttc ccataaacat
     7501 tcctgctgcg gtattgtgat taataatttt ttcgcttaat gcaggcatat aaagcctgac
     7561 gccagcaaaa acgagtaatt gaatcaccag agcaatcccg ccccatgcga aatagtctgg
     7621 aatactcacc gcattgattg ccgcactgga taaggggatc acgtacccca acaatgttcc
     7681 gctgaacgcc agtgccgccg cggtgttatt gtttttgatt aactgccatt cgttgtgcgg
     7741 tgtaattttg gagtagataa acaggaaaat aatcaccatc gccacgccaa taaaaaaata
     7801 ggcactaaag gcaagaagtg aatccagtat atgcattgta aacgtccttg taattaacca
     7861 ataatatgaa gtgatgtcat cgggatatcg acacccagcg cgcgcgaaaa taaccactcc
     7921 ggctcgccga gatcgttaaa agattcctca ccatttaaca gcagatattc gtaagtatct
     7981 ccagtcacct gccgttggta gcccatggta aaattatgca cttcccattt ggcatggttt
     8041 tgattttcta ctttttccag catgtagacg ggttcgatat ttcccggttc ttcgctgtta
     8101 aaaaatcgct gccagcgttt ttcctgccag tttaaggtca ttgcccccat cgctttggcg
     8161 ttgatcgcct cgcgccagtg actctcttta ctgataccgt agctctcttc atagacaaag
     8221 agcttgatat catcaatgtc atcaatatct tcgccgccag tggtattgat ttgcaggaac
     8281 tcatcgcccg aagtgtagta acggaaaatc tggctacccc cgcccagatc gatgcaactg
     8341 acggcggcca cggtatattc ctcacccggc agcgcaatca gcaatgcatc ttccagcaaa
     8401 cgaaacgcta acgtatcgag cgtaaaacca ctattgagat gcagacccaa cggaccgcga
     8461 gcgatagcag gcttattatc cttgccaaac agacgctgga aaaaaccaga catattttat
     8521 ccttaaaata gttgccagcc ttgcagtgag gctggcaaaa aattactcgc cctgttggcg
     8581 ttgcagtctt gctaatacat cctgggcgct acttttattg ctaccgccaa ttccggcttc
     8641 cgccagcttt tcatcgagat cgcgaccgtc tgcgactttt tccaactgtg tggcagcatc
     8701 caggcgagcc tgacgttcgg cctgacgcgt ttgcaggcgt tttaaggatt ctgccgctgt
     8761 cgaaacgctg gaagatgcgc caacagtaga ggtcgttaca gcctgttgtg cacgctgcat
     8821 ggcttcagtg gctttaacga cttccatttg ctgctcaaac tgagcgatac gctgcgctgt
     8881 cgctgtaact gctttttcca cgccatcacg agagacttcc agattcgaca acacttgctc
     8941 ttcagcggta atgagattct caaggcgcgc gatttcttca gcaacttcgt taatcaacga
     9001 cggattcacg ttcttgctta acgcttccag cgcacgagct tccagactgg ctttgcgctc
     9061 acgtaaatct ttcagcttat cgtgacttaa tttcactcgc gccagcagat caacgcgaga
     9121 ttttccggct ttatcgagtt cagtttttgc gtcacgaata tgctgttcca gcatgcgcac
     9181 gccctggttt tcttcaatgg actcttccgc ctgggagata aacgatttcc ccagcgtaaa
     9241 taaacttttt aaaatcccca taaatacccg tccttgtaat tagtgtgaat attcttctgt
     9301 aatttcagcc agatccaatg cgttatctac cagcgaagtt atctcaagca ggatatcttc
     9361 aagagaagat ttaagcgata acgcaccgaa aacaatgtaa tactcttcct gttgcacgct
     9421 ggagatccct accgatgaca acggcatcat cttctgattt cttaataaga aggtattaaa
     9481 ttcatctgga ttgctgatac tacttaccgg acaaataaag gtttcgatga tcatttggcg
     9541 ggaagtaaaa agaatattga tttgcaaatc gccataatca ttcattttaa taattaatgc
     9601 gctttcgcta tttgttacat caatattttg ttcaggtaca gtttgcagcg ccgtcgccag
     9661 cgccaacgga ttccatgtca tatattattc catatagatt aagtttaaat attaataaaa
     9721 tgaatatttg cagtacgtaa ttatcttacc agctatagac aaaaaaaaac catccaaatc
     9781 tggatggctt ttcataattc agaggaacta gctgcgctga cgaaccgctt caaataagca
     9841 aattccggtt gcaaccgaaa cgttcaggga agaaacactt cctgccattg ggatgctgat
     9901 caactcatcg caatgttcac gggtcaggcg acgcatacct tcaccttccg cgcccatcac
     9961 cagcgccagg cgtccggtca ttttgctctg atagagtgta tgatccgcct cgcctgccgt
    10021 accgacgatc cagatattct cttcctgcaa catacgcatg gtgcgcgcca gattagtcac
    10081 ccgaatcagc ggaacgcttt ctgccgcacc acaggccact tttttcgccg tggcgttgag
    10141 ctgtgcggag cgatctttcg gcacaatcac cgcatgaaca ccagcggcgt ccgcgctacg
    10201 caggcacgcg ccgaggttgt gcggatcggt tacgccgtcg aggatcagca ggaacggttg
    10261 atcgagcgaa gcgatcagat ccggcagatc gttttcctga tactgccgtc ctggcttcac
    10321 acgagcgata atgccctgat gcacggcacc gtcgcttttc tcgtcgagat attggcggtt
    10381 tgccaactgg ataaccacgc cctgagactc aagggcgtga atcagcggta acagacgttt
    10441 atcttcacgg ccttttaaaa taaagacttc ctgaaaacgt tcaggggcgc gctccagcag
    10501 ggcctgcact gcatggatgc cgtaaatcat ttcgctcatt aatgtactcg ttgtttacgt
    10561 tttgccggga gcgccacgct tgcccggcct acggataatt caccctcttc tgtttgaaga
    10621 gggtaatgat cactctgcca cttttttctt cgccgcacgc ttcgctttgg tcgctgcggc
    10681 tattttctgc gttttcgccg atggcttttt cgcttttctc gcgtctttct tcgctgcttt
    10741 cggcttcggt tttttctcgc cgcggaaggc gctgtctggc tcaaagttga cctttttgcc
    10801 gacctgacga cgcttgccgc cttttttgcc tgcatcgcct tttttcgctt tctcgcgcgc
    10861 cgttttaccg acgttgcgcg gtgcgcgttc gctggagatc aggctaaagt cgattttgcg
    10921 ctcgtccata ttaaccgctt cgacacgaac ttccacgcga tcgcccaggc gataagtctg
    10981 gccgctggat tcacccatca ggcgttgccc tacctggtca aagcgatagt agtcattgtc
    11041 cagcgaagag acatggacca gaccatcaat gaacaagtcg tccagacgga cgaagaagcc
    11101 aaagccagtg acgctggaaa ttacgccttt aaagacgtta cctacctggt cgagcatgaa
    11161 gtcacacttc agccagtcag acacatcgcg cgttgcttca tcggcacgac gttccgccat
    11221 cgaacagtgc tgacccagtt gcagcatctc ttccatcgaa taatggtagc cgccggtttc
    11281 agtggtgttg ccctgatgcc cctgctcttt cgccagcagg tatttaatgg cgcggtgcaa
    11341 cgtcaggtca ggataacgac gaatcggcga agtaaagtgc gcataggact gcaatgccag
    11401 accgaagtga ccacggtttt ccggatcgta aatcgcctgt ttcatcgagc gcaaaagcat
    11461 ggtttgcagc atttctgcat caggacgatc ggcaaccgat tccagtaact ctgcatagtc
    11521 gcgcggttcc ggcttgttac cgcccggcaa ttccagcccc agctccgcca gcactgaacg
    11581 gaaagaggta atcgcttcgg tgctcggctt gtcgtgaata cggaacagtg ccggttcttt
    11641 ggctttctca acgaaacgcg ccgccgagat attcgccaga atcatgcact cttcaattaa
    11701 cttgtgggcg tcgttacgct gagtctgttc gatacgttca atacggcgtt cagcgttgaa
    11761 aatgaacttc gcttcttcgc tctcaaatga gatcccaccg cgttcttcac gggctttatc
    11821 cagcacttta tagaggttat gtaactcttc gagatgctta accagcggcg catactgctc
    11881 gcgcagatcc tgatcgcctt gcagtatatg ccagactttg gtgtaggtca gacgcgcgtg
    11941 agaactcatc accgcttcgt agaatttgta gccagtcagg cggcctttcg acgaaaccgt
    12001 catctcgcac accatacaca ggcggtctac ctgcgggttg agcgagcaca ggccgttaga
    12061 gagcacttcc ggcagcatcg ggataacctg cgaagggaag tacaccgacg taccacgatt
    12121 acgcgcttct ctgtccagcg gcgttggcgg acgcacatag tagctgacgt cggcaatcgc
    12181 gacccataaa cgccagccgc cgccgcgttt tttctcacag taaactgcat cgtcaaagtc
    12241 acgggcgtct tcgccatcaa tggtgaccag cggtaaatcg cgcagatcga cacggcccgc
    12301 ttttgcttct tccggcactt cttctttcag cccggcaacc tgttgctcaa cagcctgcgg
    12361 ccagatgtac ggaatttcat gggtacgcag agcaatatca accgccatgc cggtgcccat
    12421 attatcgccc agcacttcga cgattttacc caccgctttg gtacggcgag tcgggcgttg
    12481 agtcagttca accaccacca caaagcccat ccgcgcgccc atgatctgat cgggcgggat
    12541 taagatatcg aagctcagac ggctatcgtc aggaaccaca aagccgacgc ccgcttcggt
    12601 gaagtagcga ccaacaatct ggctggtttt tggcaccagt acgcggacaa tacgcgcttc
    12661 acgacgacct ttacggtcag cgcccagcgg ctgagccagc acctgatcgc catgaatgca
    12721 ggttttcatc tgctcgctgg agagatacaa atcatcttta cgcccttcaa ctcgcagaaa
    12781 gccgtagccg tcacggtggc caataacggt acctttcacc aggtcgaggc gttccggcag
    12841 cgcatagcac tgacggcgag tgaagaccag ttgaccatcg cgctccatcg cgcgcaggcg
    12901 gcgacgcagg ccttcaagct gctcttcgcc ttcaatgtgc agttctaccg ccagctcatc
    12961 acggctggcc ggtttttcac gtttggttaa atgttcgagg ataaattccc gactagggat
    13021 gggattcgcg tatttttcag cttcgcgttc ctggaaagga tcttgtgaca tctcggttcc
    13081 tccgttgtca tctctgatga agattttcgt cactccacca gcaataattt ataaagcggt
    13141 tgattctctt caaccaaatc ggcaagcgtg tagttatcca gttccgtaag aaaactttgc
    13201 acggccttag aaagcgcctg tttcaaccga caggcaggtg taatgtggca aaactcactg
    13261 ctgcaattca ccagcgataa gggctccagc tcgcgcacca catcaccaat acgtattgca
    13321 ctcgccggtt tacccaagcg aatgccgcca ttttttccac gaacagcagt cacgtagccg
    13381 gcacgactaa gttgattgat tattttgacc atatgattac gggagacgcc gtagacgtca
    13441 gtcacttcag aaatactggt catccgccct tctggcaatg acgccatgta gatcagcgca
    13501 cgtaatccgt aatcagtgaa actcgttaac tgcacatcaa cctcaaaagg gaaatcgggg
    13561 aaaaatacat ttatattgat gataaaccag ccacaagctg tgtcgctaat ttatttcagt
    13621 tcggggaagg aaaaaagcga ggatttaaca ctgtgtcgga tagcgtaatg gcttaaccga
    13681 cacagcaatg tatcacactg ttagcccggc aggcaaaata tctgccaggc gtaccagaat
    13741 tacgcgtcga acgggtcgcg cagaatcatg gtttcagtac gatccggacc ggtagagatg
    13801 atatcgatcg gcacaccggt cagctcttca atgcgcttga tgtagttcag tgccgcctgc
    13861 ggcaggccac tacgatcttt cacgccgaag gtggattcag accagcccgg catggtttcg
    13921 taaatcggct ctacaccttt ccagtcgtca gctgccagcg gagtggtagt cacttcacga
    13981 ccatccggca tacggtaagc cacgcagagt ttaacctctt tcaggccatc cagaacgtcc
    14041 agtttagtca ggcagaagcc agacagggag ttcagctgta ccgcacgacg aacggcaacg
    14101 gtgtccagcc agccggtacg acgacgacga cccgtagttg cgccgaattc gttaccctgc
    14161 ttgcagagga actcgccagt ttcatcaaac agttcggtcg ggaacggacc tgcacccaca
    14221 cgagtggagt aagctttgag gatacccaaa acgtaatcaa cataacgcgg gcccaggccg
    14281 gaaccggtcg ccacgccacc agcagtagtg ttggaagaag ttacgtacgg ataagtgccg
    14341 tggtcgatat ccagcagcgt accctgcgca ccttcaaaca tgacgaaatc gccacgctga
    14401 cgcgcctggt cgagcagatc agaaacgtca acaaccatag aagtcaggat gtcggcaaca
    14461 gccatcgtat catccagaac tttctggtaa tcaaccgctt cagctttgta gtagttaacc
    14521 aactggaagt tgtgatattc catcacttct ttcagttttt cagcgaaggt ttctttgtcg
    14581 aaaaggtcgc caacacgcag accgcgacgt gccactttat cttcataagc aggcccgata
    14641 ccacgaccgg tggtgccgat cgctttcgcg ccacgcgctt tctcacgcgc gttatccagc
    14701 gcaacgtgat aatcaaggat cagcggacat gcttcggaca gcagcagacg ctcacgaacg
    14761 gggatgccac ggtcttccag ttctttcatc tctttcatca gcgcagccgg agacagcaca
    14821 acaccgttac cgatgatgct ggttacattc tcgcggagaa tacctgatgg aataagatgg
    14881 agaacggttt tttcaccgtt gattacgaga gtatggcctg cgttgtgacc gccctggtag
    14941 cgtacaacat atttagcccg ttcagtcaga agatcgacga tcttaccttt accttcgtca
    15001 ccccattggg tgcccagtac gacgacgttg ttacccattt ttcaaaatca ccgtttgctt
    15061 aaaaatggat tctaccatcg ctttttcaga gttacagcac tttttgcact caaaaatgac
    15121 ctcgatcagt ctgttttttg ttcagccaat cgttttcctc aacatgtagt agaccacaac
    15181 gcccgcaacc acaagtccac cgccaaaacg acgtaaaata ttatcgggca aattggtcat
    15241 cgcagagatc atcttcttcc atgccttcgg gtaaagcatc ggtcctaaac cttccagtac
    15301 caaaaccagg gcaagcgcca gccagattgt cgaattcatt cattatcctt atagaaaaag
    15361 aaaaccaccg acatccctga ggatgcggtg gctttattga cctgtaccgc agtcgttata
    15421 ttaacgcgtt gcggaagtcg gcgtcttcat gtagcggaag aaatcgctgt ccgggctcat
    15481 gaccatcacg tcctgattgc cagagaagct gttctcataa gcacgcaggc tacggatgaa
    15541 tgcgtagaag tccggatctt tactgaatgc atcagcaaac agtttggctg cttctgcatc
    15601 gccttcacca cgcatgatgc ggccctgacg ctcagcttct gccagcgttc tggtcacttc
    15661 atagtcggca gttgcacgca gtttttccgc ttcttcctga ccttgtgaac ggtgacgacg
    15721 cgctaccgct tcacgctcgg cgcgcatacg gttgtagatc gcttcagaca cttcggtcgg
    15781 caggttgatc tgcttaattc gcacatcgac aacttcaata cccagcgccg ccatactgtt
    15841 cgggttgatg accggaactt tgcccttcgt ctcagccgtt acgcgctctg ccgcttcggc
    15901 aatggcgtta tctgctgcag gggtagtaac ttcatcttct gtacccgcag aaccggagtt
    15961 cagcgcgtca cgtacttcga gggtcagacg accacgggaa tcggtgacga tatctttcac
    16021 gtccaggcga ccaatttcag aacgcagacg gtcagagaac ttacgtttca acagcacttc
    16081 cgcttgcgaa atgtcgccac cacccgttgc cagatagtaa cggctgaaat cgctgatacg
    16141 ccatttgatg taagagtcga cgatcaggtc tttcttctct ttggtcacaa agcggtcggc
    16201 ctggttgtcc atggtctgaa tacgtgcgtc gagcattttc accgtttcaa tgaacggtat
    16261 cttgaaatgc agacccggct cataaaccag aggtttgttg tcatcgtcac gcagtacctt
    16321 accaaaacgc agcgtaatac cgcgctcacc ttctttgacg acaaagacag acatgtaaag
    16381 cactaccagc acgatgatga taatcgcgat aactgactta cgcatcgtta ttccccctga
    16441 cgctggtagt cgttacgctg cgcgttggcg cggcgttggt ccataatatc gccctgactg
    16501 gtggacgacg tgttgcttgc tccactggtt gtggaggaag aggctggcgg cagacgcagc
    16561 agattgctgg caccgttatc gctcttcgcc gcaggggcgt taccaccttt cagcatctgg
    16621 tctaacggca gaaccatcag gttgccacct ttgtcgttaa ccagcacttt gcgggtgtta
    16681 cccaacactt tttccatcgt ctcgatatac agacgctcgc gagtaatttc cggcgcagct
    16741 ttatattccg gcagaagttt agcaaagcgc gccacttcac cctgagcttc caggatggtc
    16801 tgggccttgt acgcacgtgc ctcttcgagg atacgttgcg cctgaccgtt cgcacgcggc
    16861 tgaacttcgt tggtatacgc ttctgcttca cgaatgtatt gctgttcgtt ttcacgcgcg
    16921 gcaatcgcat cgtcaaacgc cgcttttact tcctccggcg gacgagcagc ctggaagttg
    16981 acgtccagca gagtgatgcc catgtcatac ggacgaatcg tctcttccag ttcgcgctgg
    17041 gtatcgctac gaatcacggt acgaccttcc gtcagaatgc ggtccatggt gtatttaccg
    17101 ataaccccgc gcagggcgct gtcggtagcc tgacgcagac tgtcatccgg gctggtcacg
    17161 ctatacagat atttttccgg atcggtgacg cggtactgca cgttcatctc aacgcgcact
    17221 acgttctcgt ccgacgtcag catcacacca gaagctgcca gttcacgcac ggcttccacg
    17281 ttcaccggtt tgacttcgtc gataaacgtc ggtttccagt tcagacccgg ctcaaccaga
    17341 tggctgaatt taccaaagcg tgttaccacg ccacgttcgg cttctttaat ggtatagaaa
    17401 ccactggccg cccagataat gacaatcgct gccgctgcga tggtaacgac acgaccgcca
    17461 agctgcgggc gtgggccttg cgatgaactg ccaccgccag atccggtgcc tttaccgccg
    17521 cccagaccac cgagcttttt gctcagcttg cggaagatat catctaaatc aggtggccct
    17581 tgatcgcgac cgcctttgtt tccatttccc tcagagttgc cgccaggttt gctgcttccc
    17641 cacgggtcgc ggtcttgtcc gttattaccg ggctgattcc acgccatgtt tgtgctccat
    17701 atttgttatg cggtgatccc tgttgtcttt cgacgccaga ggatatgact ccacgcttca
    17761 gacgctacgc cgttagatca ggtaatcgat caacgccggt tcttgtttac agaggcgacg
    17821 ccagtcaacg atcggcatac gaacttgcag acttacgctg ccgtcctcct ccatccactc
    17881 tttttctatt gcctgaagct gataaaaacg acttctcaga cgcccttcct gcggtggcag
    17941 acgcaatgta tgctgcgcca cctcgccgga aagccgctcc gtcaaagcct gaaaaagctg
    18001 tggtatcccc gctccggtct gtgcggaaag ccagacacgg atcggtttgt tctcttcgtc
    18061 ccgatcaata cgcggttcga aatcttccag catatcgatc ttgttcatca ccagcagggt
    18121 tgggatctcg tgagcgtcga tctcttcaag aaccgtattc accgcttcga tgttttcttg
    18181 tacacgcaca tccgccgcgt caatgacgtg cagcagtaat gtggcttgcc gcgtctcttg
    18241 taacgtggct ttaaatgccg ccaccagatc gtgcggcagg tggcgaataa accctacggt
    18301 atctgcaagt acggtttcac cgacatccgc gacgtcaata cgccgcaacg tcgggtcgag
    18361 ggtggcaaac aactggtctg ccgcgtagac ccgcgcttcg gtgatgcgat tgaaaagggt
    18421 agatttaccg gcgttggtat atcccaccag cgaaacagta ggaacgtcgg ctttgatacg
    18481 cgattgccgc ccctgctcac gctgcttttc aactctttcc aggcgcgact gtatctgcac
    18541 gatgcgatta cgcaacaaac gacggtcggt ttcgagctgg gtttcacccg gaccacgcaa
    18601 accgatcccg cctttctgtc tttcaaggtg ggtccagcca cgcaccaggc gcgtagccag
    18661 atggcgcaac tgcgccagct caacctgcaa cttaccctca tgggtacgcg cacgttgggc
    18721 gaaaatatct aaaataaggc cggtgcggtc gatgacacga cactcgcaca aacgctccag
    18781 gttacgctcc tgcgccgggc tcagggcatg gtcaaaaaga acgaccgaag ctcccgtcgc
    18841 ttttacggct tccgcaattt caactgcttt accttcacct acaaaatact ttgggtgcgg
    18901 cgctttacgg ctaccggtaa tcacctgcaa tgcttcgaca ccggcggaag agaccagaga
    18961 ttcaaactcc tggaggtctt ccatatcttt gtcttgcgta aaatagatgt gtaccagtac
    19021 cgcctgctca ccagcatcat aacggtcaaa caagcgtata accctctaaa tagatcagcg
    19081 gggaacgcag gatcgctggc tccccgtgta aaaaaacagc ccgaaacctt attcggtttc
    19141 ttcgctgtcc tgttgcgcgg aagtattctg cgcgctgcta ccatgatggt agttactgct
    19201 ggtaccgcca ccggcgttgt tactgtgatg agaaaccggg cgagacggga caacagtaga
    19261 aatcgcgtgc ttgtaaacca tctgactgac cgtgtttttc aacaggatca cgaactgatc
    19321 aaaagactcg atttgccctt gcagcttaat accattcacc aaataaatag aaactggaac
    19381 acgttcccga cgcagtgcgt tcaggaacgg atcttgtaaa gattgcccct tagccattct
    19441 ctcttttcct tatatgctta tttgtacttt gaacctttcg attctgaaaa aattgcgcac
    19501 gatacgtctc aattgtacac attcagcctg cgatagcacc aacaacctgt aatacttcgt
    19561 cacgcgcttg ttctggtttt tcactgtcaa gccagtgaac cccttcccaa ccacgcagcc
    19621 aggttatctg ccgcttcgcc aactgtctcg tggcgcaaac acctcgataa accatttcat
    19681 cgtatgagat ttcgccttca aggtaagacc acatctggcg ataacccacg caacgaatgg
    19741 aaggcaaatc cgtatgcaaa tctcctcggg caaaaagcgc ccggacttct gcttcaaaac
    19801 ctgaagccaa catctgatga aaacgctgct caattcgttg atggagcagt tcacggctcg
    19861 ccggggcgat ggcgaactga tgcacctgat acggtagagc gtctcctgac gtttgcgtca
    19921 gttccgttaa agttttaccc gaaatgaaaa aaacttccag tgcccgggaa agcctttgtg
    19981 gatcatttgg atgaatcctt gctgccgcaa ccggatcgat ctcctgaagt tgacgatgca
    20041 atgactccca accttgctct gccgcctgtt gctcaattct ggcccgtact tccgggtctg
    20101 ccgacggtag cggcgacaac ccttccagca atgccttgaa atacaacatc gtaccgccca
    20161 ctaacagtgg gatccgcccc gccgcggtga tatcggccat ttccgccagc gcatcgcggc
    20221 gaaaatcagc agccgagtaa gcctgcgacg gatcgcgaat attcagcaat cggtgcggcg
    20281 cggcgagtaa ctcttcagcg ttcggcttcg ccgtcccgat atccatccct ttgtaaataa
    20341 gggcagaatc aacgcttatc aactctactg gtaaaatttt acgcagctca atggctaacg
    20401 ccgttttacc ggaggccgtc ggccccatca aaaaaatcgc cttaggcagg ctcgccttac
    20461 tgatatcact catctttcag ggcttttatc gccagatgta aatcaacaga ttgtaacaga
    20521 ccacccggcg gcgttttcac aagttgcgga cataaccgtt ccacgtccgc cagcagggtt
    20581 atggcctgtg ccattgacca ctgcgcatgt tcgctcatca gatttcgtgc aatccactgc
    20641 gcaatattgc caggttcgaa tacggactgc ttcgccaggt agcctatcag ttcaggaatc
    20701 aagatttgta aattttgttg gcgtaagggt aaaggcacgg ccctgatggt cacatgctgc
    20761 gcatcggact ggaaatcaat acccaattcc gccagggcag actgcgcttt ttctaatgcc
    20821 gatttttctt cgccagaaac ttttagccgc aacggaataa gcagcggttg ggcgcaaact
    20881 ggcacttcac ccggcgtcaa ttgtgcctga cgcagccaac gttctgccac tggcaaggat
    20941 aaaagtgaaa tgttgccatc gcgctcaagc aacgcacagt cggaatggac gatagtcagt
    21001 acccgaccaa aactctgact gttcgccgca agtgcaggtt cctgcggttc tggcgctttt
    21061 ggtttttgca tcggagcggg cgtttgcaaa agctggcgat acacttcacc ttgctgtttc
    21121 tggtaaccag gctgcgcatt cggccaggag gcagccggac gactgcctga ggcgggcgca
    21181 ggagagtagc gcggagctac cggctcacga actgccggtt cagcaaagtg attgcgccca
    21241 gccgccacgc gattttccgg aatggcacgc ggtgcaggtt gtggttcatc gtccagcggt
    21301 agcggcgttt ccagttgctg ttgtagcacg ctcagcacgc cctgatagat aaagtcatgc
    21361 accagacgcg actgatggaa acgcacttcg tgtttggcag ggtgcacgtt gacgtccacc
    21421 tgatgcgggt cgatctccag atacaacaca aatgccggtt gctgattggc ccccagtttg
    21481 tcttcgcagg cctggcggat cgcgtgattg atcaggcgat cgcgcatcat gcgaccgttc
    21541 acgtagcaat actgaatttc tgccagtgcg ggcgtagtgt gatttggatc ggccacccag
    21601 ccgcgcagcg tgagatcgcc gtgttgccat tcaatcgcca gcgcctgttc gagaaaagcg
    21661 gtgccgcaaa tcgcgcctaa gcgccgttct ttttgcccgc cttccggcac tgcgcggtac
    21721 tggcgtacaa ttttgccgtt atgcgacagg ttgatagtga cgtcgaaacg cgccagcgca
    21781 atgcggcgga tgatttcatc gatatggtta aattcggttt tctcggtgcg caggaatttg
    21841 cgccgcgccg gggtgttgta gaacaggtcc agcacctcca gcgtcgttcc taccggatgc
    21901 gccgccggtt taaccgtcac gtccatatcg cgcccttcgg cataggcctg ccaggcttcc
    21961 tgctgttccg cagtacgcga agtgagcgtc aaacgagaaa ccgaactgat actcgccagc
    22021 gcctcaccgc gaaagcccag gctgataatg gcttcgagat cgtccagaga ggcgatttta
    22081 ctggtggcat gacgcgccag cgccagtgcc agctcatctt ttttgatacc gcagccgtta
    22141 tcacgaatgc ggataagttt cgccccaccg cgttcgatat caatatcgat acgcgtcgca
    22201 cctgcatcga ggctgttttc caccagttct ttgactaccg acgcaggtcg ctcgaccacc
    22261 tcacctgcgg caatctggtt cgccagttgt ggcggtaaga cctgaattgg catcaatcgt
    22321 ccttagtttg gcagcgtgcg atctggcgtc gtcaccgtac tggccgtttg tgccgttgca
    22381 ccctgcggcg cagattgcat cggatgcgca aggaagtaat tgcgcaggcc tttgtaaatg
    22441 gcttctgcca gctgttgttg gtaatcgtcg ctcgccagca aacgttcttc gctgttgtta
    22501 ctgataaaac cggtttcgac cagtactgac ggaatatccg gcgaacgcag aacgccgaga
    22561 ctcgcgtgtt ctggtcggcg tttatgtatt tcgccaatgc gttgcaactg actgatcata
    22621 ctggtcgcta catcataccc tacccgctgg gaatgaccga actgtaaatc cagcaccgcc
    22681 tggcttaaat aggggtcggc ctgactgttc gccagcacat cacccgcccc gcccagcagc
    22741 tccgactgtt tctcgtgctg ctccagccag ctggccattt cgctgttggc gcgacggtta
    22801 gagagcaccc atacggaagc accggtggca ctacggtttg gtgcggcatc ggcatgaatc
    22861 gacacaagga aattggcgtt ttgcttacgt gccacatcgc tgcgccccat caccgaaata
    22921 aagtaatccc catcacgggt taaaacgcct ttaaacatcg gatcgtcatt gagcaaggta
    22981 cgtaatttac gcgcaatggc gatggtgaca tttttctccc gcgtaccgcc ggggccgata
    23041 gcgccagggt cctgaccgcc gtgtccggca tcaatagcga tgataatttt atcgccagtg
    23101 ttagccgtcg cgcgcgcagc cggtcgcgtt accgtattac tgctgataac acccgtagtg
    23161 cggttacttt cagttttaaa cggattgcgc gctggttcac tgacgcgcgg tgcgacaacc
    23221 gcaggcgttt caacgcgttt cgcaaccaca ggcggcggag gaggcggtgg cggcgcatcg
    23281 gcgttaatcg taaagacgac agtgtaattg ctgccattct gccgcttcac cgcttcggtt
    23341 ttaccatttt cggtaagatc gaccaccagc cgtagcgttt gtgcatcttt aggcgttccg
    23401 gagcgaatcg ccttcaccag attattgccg ctgaacaaca acggcagccc ctgaatcacg
    23461 cccgtttgtt tgatatcgag cgccacggtg cgtttgcttt gatggctaaa cgcataatca
    23521 ggatcgccaa taaaactcaa cgttatccgc gcctgttgat tgccgttaga aacctgaata
    23581 tcagagagcg tcgcggcacc caccggcgcg cacagcagca gcaacgtcgc taccaaccaa
    23641 tttctgatgc gatacatcat cctgccacct ttcaaaggtt aaccggctaa acgcgccagc
    23701 aacaattcac ccgcagagga aaccgcactc acgcgcgcct cacggccttg tgcctgataa
    23761 tcaatgtgta tttcgacatc cgggtcagga agaacacctg taccttgttg tggccactcc
    23821 accaggcaga tggcatcgtt ggcaaaataa tcgcggatcc ccataaactc cagctcctcg
    23881 ggctcggcaa ggcggtacaa atcaaagtga taaaccatta aattgtcgag cgtatagggt
    23941 tcgaccagcg tataagtggg gcttttgaca ttaccctgat gacccaaagc ctgtaaaaag
    24001 ccccggctaa aggtggtttt acctgcgcct aaatcgccat acagatagat tacggttgca
    24061 ccatcgcagg ctttcgctac ccgctcgccc aggtctaatg ttgcctgctc atcagggagc
    24121 ggaattactc gattcatcat ggtttttatc agtcacttcc gggttaacaa tacgctgtag
    24181 cgtggaaaag agatcggttg ccagcatccc gcgcgttcca aaacgcgccg ccagtacgtc
    24241 agctgccgca ccgtgcgcga cacagcccgc acaggctgca tcatacggcg acagtttttg
    24301 cccaagcaat gcgccaataa taccagagag cacatcgccc atgccgccgc tcgccatgcc
    24361 tgcattcccg gcatcaataa tgcctaaagc gtcaggatgg gcggcgacca cggttccggc
    24421 acctttcagc accgctacgc cgccataacg ttgtaccaga cgttgggtgc aatgtaagcg
    24481 gtcactttca atttcagcga cggaacagcc taacaaccgt gcggcctcgc caggatgcgg
    24541 cgtgatcacg cgattgtgac gcttatcggg attgattgcc agcaggttca atgcatcggc
    24601 atcccacaac atcggtttgc gaaaattctc aactttttgc agggcttttt tcccccactc
    24661 ttgctggccc agaccgggac caatcaccac cacatcggcc cattccaggc tttcggtaag
    24721 agagtccata gtcagttcat gcaccatcaa ttccggtcgt gcagtcagca gcggcgcaat
    24781 gttctcactg cgggtcagta ctcggactaa accagcacca gcacgcagcg ccgcttcccc
    24841 cgtcatacga atagcccccg ccgtgccgtg atcgccacca ataatcacca gccgcccgtg
    24901 atcgccttta tgcgaagtcg ggcgacgcgg tatcagccac tgagaaagtt gttctgccga
    24961 aaaccgctga attttcgtct cctgacctgc cagccagcta tccagcccca gtgagtcaaa
    25021 atgcagttgt ccggtaacat cccgcgcttt tccagtgagc aagcctggtt tcagcgcaat
    25081 aaaagtgatg gtgtgatcgg cgttgattac cgcgcctggc gtagcgccgg tttcagccag
    25141 aaggccggaa gggatatcaa ccgccacaat cggcgcaggg tgagtattag cgtggtcgat
    25201 taactggcta atggattcgc ggggcgcttg ctgcaagccg gtgccgagca gcgcatcaac
    25261 aatcagatct accgattcgg gccagacaat attcgaagca tggatctcgc ctcccgcgtt
    25321 taaccatgct tcgcgtgcca gcgcggcctc ttccggcaac ggtttgtcac tttcctgggc
    25381 cagcagcgtg acatcaatac cgatcgcttt ggccagccgc gcgaccacgt agccatcgcc
    25441 gccgttatta ccatgaccgc acagcaccag ccagtggcgg gcgtcaggat aagccgaacg
    25501 acacacctgg aatgcggctt cgccagcgcg aagcatcagc tcatagagtg tgagccccag
    25561 cgcatctgcc gcctcgcgtt ctccgcggcg gatatggtcg gcgtaccaga ctgtgtgtgg
    25621 tatacttacg gggtttttct tcattgtatg gtccgtcatg tcagagcccc tcgatctcaa
    25681 tcaattagcg caaaatatta aacagtgggg gctggaactg ggctttcagc aggtaggtat
    25741 taccgatacc gatctcagcg cgtccgagcc caaactgcaa gcatggctgg acaaacaata
    25801 ccacggcgaa atggactgga tggcgcgtca cggtatgcta cgtgcccgcc cccatgagtt
    25861 attgcccggt acgctgcgcg tgatcagcgt gcggatgaat taccttcctg ctaacgccgc
    25921 atttgccagc acgctgaaaa aaaccaaact cggctatgtt agccgttatg cgctgggccg
    25981 tgactatcac aaacttctgc gcaaccgact caaaaagctg ggcgagatga ttcagcaaca
    26041 ttgtgtttcg ctgaatttta gaccgtttgt cgattctgcg cctattctcg agcgcccgtt
    26101 agctgaaaaa gcaggtcttg gctggacagg taagcactca cttatcctca atcgcgaggc
    26161 cggttcgttc ttctttttag gcgaattgct ggtcgatatt ccgctgcccg tggatcaacc
    26221 agtcgaggaa ggatgcggta aatgcgtggc ctgtatgacg atttgcccga ccggtgccat
    26281 cgtcgagcca tataccgtcg atgctcgccg ctgtatctct tatctcacca tcgaactgga
    26341 aggggcgatc ccggaagagt tgcgaccgtt aatgggaaac cgtatttacg gttgcgatga
    26401 ctgccagctt atctgcccgt ggaatcgcta ttcgcaactc actacagaag acgatttcag
    26461 cccgcgtaag ccgctacacg caccggaact cattgagtta ttcgcctgga gtgaagagca
    26521 gtttttaaaa gtcacggaag gttcggcgat tcgccgtata ggtcacctgc gttggctgcg
    26581 taatatcgcc gtagcattag gcaatgcccc ctgggatgaa acgattttga cagcgctgga
    26641 aagtcgtaaa ggtgagcacc cacttcttga tgagcacata gcgtgggcga ttgcgcagca
    26701 aatcgagaga cgaaatgcgt gcgtggtcga agtgcaatta ccgaaaaaac agcgtctggt
    26761 tcgggtgatt gaaaaagggt taccgcgtga cgcctgattc atccacagct tgtgaataaa
    26821 aataaaaacg cattgcaatt caagacgaat aaaatcgtca agtgatcgac tcgacaaaac
    26881 ggaatcttaa tttttaactt atctatcaat aggttaaaaa aatactattc accgtgcgaa
    26941 gtttctttga aattaagatt acagcctgag actgtggata actctgttta caagagtttg
    27001 tcagagacaa caaaaaccag caataacgca tcgcgtcgct gtggatattt tattgagaga
    27061 agaatttgga gcgggaaacg agactcgaac tcgcgacccc gaccttggca aggtcgtgct
    27121 ctaccaactg agctattccc gcgtactact taattttgct ttcgtaattt tcgcatttct
    27181 gcgtcgttac gggaggcgca ttatacgaga aatctttccc tctgcaaccc ccctaaaaaa
    27241 ttttttttga gtttttatct caagtgctga aattatcagc aagacgttca atttagcgtc
    27301 aaaatcacgc cgccgcaagt ggattccggc gcttacgtga ccaggaaaaa ttttacagct
    27361 tgataaaatg ctcgcggtag taagccaact ccgccaccga ttcacggata tcatccatcg
    27421 cctgatgcgt cccctgcttg gtaaagccgt caagaatttc cggcttccag cggcgcgcca
    27481 gctctttcag ggtgctaaca tcgagatagc ggtagtggaa gtaggcttcc agctccggca
    27541 tgtatttaaa caggaagcga cggtcctgac cgatgctgtt accacaaatc ggcgattttc
    27601 ccgcaggcac ccactgtttt aagaattcga gcgttgccag ttcagcttca cgatcgccca
    27661 tcgtgctcgc tttcacacgc tctaccagcc cgctggcggt atgggtgcgc acgttccagt
    27721 catccatcag cgccagctgt tcatcggact gatgtactgc aatggtcggc ccttctgcca
    27781 gaatattcag gttggcatcg gtcaccagcg tggcaatctc aataatgcga tcgcgctcgg
    27841 gatccagacc ggtcatctca agatcgatcc aaatcaggtt gttttcattg gcactcatgc
    27901 tattttccac ccattcacag tgactatatt gatccacaat tgcgtgtatc atagatgttt
    27961 tgcccatcag gggcgaccag gagtctgtac gattgagtaa aaataaactc tccaaaggcc
    28021 agcagcgccg cgtgaacgcc aatcaccagc gtcgtcttaa aacgtctaag gagaagcccg
    28081 actacgacga caatctgttt ggcgagcctg atgaaggtat cgtcatcagc cgctttggta
    28141 tgcacgctga tgtggagtcc gccgatggcg acgttcaccg ctgcaatatt cgccgtacca
    28201 tccgttcgtt ggtgaccggc gaccgcgtag tctggcgtcc gggtaaacca gcggcggaag
    28261 gcgtaaatgt caaagggatc gtggaagcgg tgcatgagcg cacctcagtg ttgacgcgcc
    28321 cggattttta cgacggtgtg aaacctattg ctgccaacat cgaccagatt gtcattgtct
    28381 ccgccatttt gccggagctg tcgctcaata ttatcgaccg ttacctggtg gcctgcgaaa
    28441 ccttgcagat tgagccgatt attgtgctca acaagataga cctgctggac gacgaaggca
    28501 tggagttcgt caacgagcag atggatatct accgcaatat cggttatcgc gtattgatgg
    28561 tttccagtca tactcaggat gggctgaaac cgctggaaga ggcgttgacc gggcgcatca
    28621 gcatttttgc cgggcagtct ggcgttggca aatccagcct gctgaacgcg ttgctggggc
    28681 tgcaaaaaga gatcctgacc aacgatgtct ctgacaactc ggggcttggt cagcacacca
    28741 cgaccgccgc acgactgtat cacttcccgc acggcggtga tgtgattgat tccccaggtg
    28801 tgcgtgagtt tggtctctgg cacctggagc cggaacaaat cactcagggc tttgtcgaat
    28861 tccatgacta tttaggtctg tgtaaatatc gcgattgcaa acacgatacc gatccgggct
    28921 gcgctatccg ggaagcggtt gaggaaggga aaatcgcgga aacccgtttc gaaaactatc
    28981 accgtattct ggaaagcatg gcgcaggtaa aaacgcgtaa aaacttttct gatacggatg
    29041 actgacaatt aagctaaccg tcgttagaat cgtccccctt tttttcagga tccggcatgt
    29101 aggccggatc aggaacgaca aaacaatggc ctggaggcta ccttgttaaa ttcatttaaa
    29161 ctttcgctac agtacattct gccgaaacta tggcttactc gcctggcggg ttggggcgca
    29221 agcaagcggg caggatggct gacaaaactg gttatcgatc tgttcgttaa atactacaag
    29281 gtcgacatga aagaggcgca aaagccggac accgccagct accgcacctt taacgaattc
    29341 tttgtccgtc cgctgcgtga cgaagtacgc ccaatcgata ccgatccgaa tgtactggtt
    29401 atgcctgccg atggcgttat cagccagttg ggtaaaatcg aagaagataa aatcctgcaa
    29461 gccaaaggcc acaactacag cctcgaagcc ctgctggcag gcaactatct gatggcggat
    29521 ctcttccgta acggtacgtt tgtgaccact tacctctccc cacgtgacta ccaccgcgta
    29581 cacatgccgt gcaacggtat tctgcgtgaa atgatctacg tgccgggcga tctcttctcc
    29641 gttaaccatc tcacggctca gaacgtgccg aatctgtttg cccgtaatga acgcgtgatc
    29701 tgcctgttcg ataccgaatt tggcccgatg gcgcagattc tggtcggtgc gacgattgta
    29761 ggcagcattg agacggtctg ggcgggcacc attacgccgc cgcgcgaagg catcatcaag
    29821 cgctggacct ggcctgccgg ggaaaacgac ggttctgtgg cattgctgaa aggccaggaa
    29881 atgggtcgat ttaaacttgg ctctaccgtt atcaacctgt ttgcaccagg taaagtgaac
    29941 ctggttgagc aactggaaag cctgtctgtc acgaaaattg gccagccgct ggcagtatct
    30001 accgaaacct ttgttacgcc agacgctgaa ccagccccgc ttcctgctga agagatcgaa
    30061 gcagaacacg acgccagccc attggttgac gacaaaaaag accaggtcta atccatcaaa
    30121 ggaaacgctg acgtgcgcct gattatcact tttctgatgg cctggtgcct cagttggggg
    30181 gcgtacgccg cgacggcccc cgatagcaaa caaatcaccc aggaactgga gcaggcaaaa
    30241 gcggcgaaac ccgcgcagcc ggaagtcgta gaggcgctcc agtctgcctt aaatgcgctt
    30301 gaggaacgaa aaggttccct tgagcgcatc aagcaatatc aagaagtcat tgataattat
    30361 ccgaaacttt ccgccactct gcgcgcgcag ttaaacaaca tgcgtgacga gccacgcagc
    30421 gtgtcgccgg gaatgtctaa cgatgcgctg aatcaggaaa ttctccagat cagcagtcag
    30481 ttgctggata aaagccgtca ggcccagcaa gagcaggagc gcgcccgcga gattgccgat
    30541 tcgctgaatc aactgccgca acagcaaacc gatgcccgcc gtcagttgaa tgaaatcgag
    30601 cgccgcctgg ggacacttac cggcaatacc ccgctcaatc aggcacaaaa tttcgcgttg
    30661 cagtctgact ccgcacgtct gaaagcgctc gttgatgaac tggagctggc gcagctatct
    30721 gccaataacc gccaggaatt agcgcgcttg cgctctgagc tggcggaaaa agagagccag
    30781 cagctggatg catatttgca agcactacgc aaccagttga atagtcaacg ccaactggaa
    30841 gcggaacggg cgctggaaag taccgagcaa ctggcggaaa gtagcgccga tttgccaaaa
    30901 gatatcgtcg cccagtttaa aattaaccgc gaactatcgg cggcgctgaa tcaacaggcg
    30961 cagcggatgg atctcgttgc ctcgcaacag cgtcaggctg ccagccagac gttacaggtc
    31021 cggcaggcgt tgaatacgct gcgtgaacag tcgcaatggc tgggatcatc taacctgctc
    31081 ggcgaagcgt tgcgggcgca ggtggcacgg ctaccggaaa tgccaaaacc acaacagctt
    31141 gataccgaaa tggcgcagtt acgtgtgcaa cggttacgtt atgaggatct gcttaataaa
    31201 cagccgctgc tacggcaaat ccatcaggcc gacggtcagc cactgaccgc tgagcaaaac
    31261 cgtattctgg aagcacagct acgcactcag cgtgagttgc tgaactcatt gttgcagggt
    31321 ggcgacacgc tactgctgga gctgaccaag ctgaaagtct ccaacgggca actggaggat
    31381 gcgctgaaag aggtgaacga agcgacgcac cgctatctgt tctggacctc tgacgtgcgc
    31441 ccgatgacca tcgcctggcc gctggaaatc gcccaggatc tacgtcgtct catttcgctg
    31501 gacaccttca gtcagttggg caaagccagt gtgatgatgc tgaccagtaa agagacgatt
    31561 ttgccgctgt ttggcgcgtt gattctggtc ggttgcagta tttactcgcg ccgctatttc
    31621 acccgttttc ttgaacgttc ggcggcgaaa gtcggcaaag tgactcagga tcacttctgg
    31681 ctgacgttac gcactctttt ctggtcgatt ctcgtcgcgt caccgctgcc tgtgctgtgg
    31741 atgacactgg gttacggctt gcgcgaggcg tggccttatc cgctggcggt cgcgattggt
    31801 gatggcgtaa cagccaccgt gccgctgctg tgggtagtga tgatttgcgc cacctttgct
    31861 cgcccgaacg gcttgtttat cgctcatttt ggctggccgc gcgaacgtgt ttcccgtggg
    31921 atgcgttact acctgatgag catcgggctt attgtgccgc tgattatggc gctgatgatg
    31981 ttcgataacc tcgacgaccg tgaattctcc ggttcgctgg gacggctttg ctttatcctc
    32041 atttgcggtg cgctggcggt ggtcaccctc agcctgaaaa aggccgggat cccgctgtat
    32101 ctcaacaaag aaggcagcgg cgacaacatt accaaccata tgctgtggaa catgatgatt
    32161 ggcgcaccgc tggttgccat tctggcgtcg gcggtgggtt atctggcaac ggcgcaggcg
    32221 ctgttagcga ggcttgaaac ctcggttgcc atctggtttt tgctactggt ggtttatcac
    32281 gttatccgcc gctggatgct gatccagcgc cgcaggctgg cgtttgaccg ggcaaagcat
    32341 cgccgggcag agatgttagc gcagcgtgcg cgtggcgaag aggaagcgca tcatcacagt
    32401 agcccggaag gagcaattga agtcgatgaa agcgaagtcg atctcgatgc catcagtgcg
    32461 caatccttgc ggctggtgcg ctcaattttg atgttgatcg ccctgctttc ggtcattgtg
    32521 ctgtggtcag aaatccattc cgctttcggc ttcctcgaaa atatttcgct gtgggatgtc
    32581 acttccacgg tacagggcgt agaaagcctg gagccgatta ccctcggtgc ggtactgatt
    32641 gccattctgg tgtttatcat caccacgcag ctggtgcgca acctgcccgc gctgctggaa
    32701 ctggcgattc tgcagcacct ggatttaacg ccgggtacgg gctacgccat caccaccatc
    32761 accaaatatc tgctgatgct gattggcggg ctggtcggct tctcaatgat tggtattgag
    32821 tggtcgaaat tgcagtggct ggttgccgcg ctcggtgttg gtctcggttt tggtttgcag
    32881 gaaattttcg ccaactttat ctctggcctg atcatcctgt tcgaaaaacc gattcgcatt
    32941 ggcgatacgg taacgatccg cgatcttacc ggtagtgtga cgaaaattaa cacccgcgcc
    33001 accaccatca gcgactggga ccgcaaagaa attatcgtgc cgaacaaggc gtttattacc
    33061 gagcagttta tcaactggtc gctctctgac tcggtcacgc gtgtggtgtt gacaattccg
    33121 gcccctgccg atgccaacag tgaagaagtg acggaaatcc tgctcaccgc agcgcgtcgc
    33181 tgctcactgg tgatcgacaa cccagcaccg gaagtcttcc tggtggatct gcagcagggg
    33241 attcagattt tcgagctgcg tatttacgcc gctgagatgg gtcaccgtat gccgctacgc
    33301 catgagatcc accagctgat tctggctggc ttccacgccc acggtatcga tatgccattc
    33361 ccgcccttcc agatgcgtct ggaaagcctc aacggtaaac aaacggggag aacattaact
    33421 tctgcgggca aaggtcgtca ggcgggaagt ttgtaaggtc tttgtggccg gatgaaccca
    33481 tccggctcac aagcaaaaat gatagcgcag aaaaaactgc cacagccagt ggccgaagat
    33541 cgccagggtg gtagcgtaca taaaccagcc gcgttttttt cgccacagcg cgaaccagaa
    33601 aaaggggaaa aagagcggta atgtcaatat cacaccgaca tcacgggtat cgtcattttc
    33661 cagatactcc agcacatcgc agatgttttt aaccccgcct tcatcaatca tccactcttt
    33721 gtccagcgtg gaagtgatga gtatccaggg gatcgccaca acaaaccaga tgcagcacaa
    33781 gataaacatg cgcgcactca tgagataatc ggtcgcttaa tttcgctata gcgcatacga
    33841 aacaaatcgc cgtatcccac taattctccg cgaaaaacca gacatctaat aagataagaa
    33901 gataagcgca atagatatcc ggcaggtttc cccaccgcgg accatcgcgc tctttgaggg
    33961 tattaatagt gttacccacc accatcgcta ctttgcgggt gtggcgggta tcgacaaaag
    34021 agaggaccgt ttgctctatt tccgccacat ccccggcaga aaattgcagc tccctgattt
    34081 catcttcggt tatcactgga tcccgactag cgtcgtccat aacgtcttcc tttccagaca
    34141 actcatcgcc ggagagacga tgagttgcag gatttcatat gatcagtcga gagctaactc
    34201 aggtttgttc gccatgcgac tgcaatagtt ctggtaaatc gccatcgcta acagcgagaa
    34261 gaagacaggg ccgccaatca tccacaatgt gctgtcccag tctccagctt caaccacagg
    34321 ttgaatgatg gtgaagacgt tcgcaaatgc caccaccagt acgacaacca ctgttgcaat
    34381 cattgccgac atatgcgttt tgaaaatcac aaacggtctg tcgagatcct gacgtgcttt
    34441 aaaaaatggg aaagccagcg cgaggaacag gtaaggaagc gtcatagaca cgttcgccat
    34501 cagcgtcagc ttgttaaaga acgccgatgc ggtaccgcca ccaaacgaaa ccagcaggat
    34561 gaagacagta accaacccac actgcatcca catggcgata gacggcatcc ccatcgcatt
    34621 caggcgcgtc atcggttccg gccacaatgc tttcggcgtc ccctggatga tggctttcag
    34681 cggtgaatag caaagcgtaa agaacgcacc ggtataagcg aggaacatcg acagtccggt
    34741 aatacgcgca aaccatacgc ccagcgacaa tgacgcttca ggtgacaaat gcagtgcgtt
    34801 acccagcgtc acccctaggc tcttcatcag cacataggta atattgccga ggttaacgga
    34861 accattactt aatacctgct gccagtttgt gctgacgccc cataaaaata ttgccagcga
    34921 ataaccgatt gaaataacaa tagcggcaaa aacaatacct ttggcaaagt tcttttctgg
    34981 attttccgtt ttatcaacca gaccaccaac cgcttcaatt ccgccatagg caaaaatggc
    35041 aaataccaca aacgatagca ttgccagacc ggactgataa cctgggttcg gtgatgcaag
    35101 gaaattaata tcctgcgcga aatgcccacc atttaataac aaaatagtaa tgcttactaa
    35161 cagcaatact aaattcagac acattactgc aataccgccc accgcagtaa tgcgggcaat
    35221 tttattaatc ccttttgaag caacgacggt gaccagaatc atccatgcca ctgccagcag
    35281 accaaccacc tgcgtaggct ccagtccggc aatatgccag tgctgggtca tgtcgctacc
    35341 gtagaggaat gttgagaacg gtacccaaac tttcgcggag gtactcacca tccagatgat
    35401 ataagaggaa aaccacataa acgtaccaat gaaggcaaaa cgtggtccga cactattatt
    35461 catccaggaa tagataccat cttcttcttt gcgataagca gctcccattt cagccatcat
    35521 taaggcgaat ggaataaaga ataacaatgc agaaaatata taaaagggaa tcgcactata
    35581 acccattaag taataagccg atgggctatt ggcaaatcca aatacggaag taaagatcat
    35641 caatatgagt cctatcagac tcatcttttt tatcgtgtga ggcatgaaac catccttcat
    35701 ttcagcgcgt gtataaaagg cgcccactga actgcactgc ccaacattga gcgggcagcg
    35761 taaaaaccaa tacagaattg atacctggat gatatcaaat atccagtctt aaattgtggc
    35821 ctttagcgga aaaatgaaaa catatgctac agaatggcgc ttatcacgcc attcttcgct
    35881 gttaattcag taatttttca gaattatgcc cggtcaacgc taaaggcgat gacttcagcc
    35941 agtgtctccg cgcccagcgc caacatcacc agacgatcaa cacctaatgc cacgccggaa
    36001 cagtcaggca taccgacttt caaggcttca atcagattct ggtcaatggg gtgctgcggc
    36061 aaaccgcgcg ccgcgcgctt acggttatct tgttcaaagc gttgttgctg ctcacgggca
    36121 tccgtcaatt catggaaacc attcgccagc tcaatacctt tataataaac ctcaaagcgt
    36181 tcagcgaccc gatgatcttc ggtactgatt tgcgccagtg atgcctggct ggctggaaag
    36241 tggtacacaa aggtcggttt ttctttgcca atatttggct ctacgccaaa ggtaaacagc
    36301 aattgtagaa gcgtgtcgcg gtcttcttca gtatcggcaa cgttgctcaa atccagtttc
    36361 gctgccactt cccgcagttg cgttttgtcg gcagatagtg ggtcaatttc cagataacgc
    36421 aagaaagctt gttgataaga aaggctttct gctgccggac agtccagcac ctgttgcaag
    36481 agatcgtcca cctcgttcat caaccggtac atatcatagt gcggtcgata ccactcaagc
    36541 atagtgaact cagggttgtg ataacgcccc atctcttcat tacggaagct gcggcacagc
    36601 tggaataccg gcccacaacc ggcaaccagc aggcgcttca tatggtattc cgggctggtc
    36661 attaaccaga gattcatccc ctgcgaatgc ccggggccaa cgaaacgtgt ctcaaacggg
    36721 accaaatgaa tatcggttac cgtcgcctgg ctcatacaag gcgtctccac ctccagcact
    36781 ccacgatcgg caaagaaacg acggatctcc gccataatcg ccgcgcgttt taataagtta
    36841 ggaatggatg cgctcggctg ccaggatgcc gtttcgctca tagttaaatc tccagttttt
    36901 gacaagggca cgaagtctac tcgcaacgcg acggcgagac aaattttacg caggaataaa
    36961 acaacggtgg gcagtgacta aaaaaagcac gatctgatgg tttagtaatt aaattaatca
    37021 tcttcagtga taatttagcc ctcttgcgca ctaaaaaaat cgatctcgtc aaatttcaga
    37081 cttatccatc agactatact gttgtaccta taaaggagca gtggaatagc gttcgcagac
    37141 cgtaactttc aggcacttac cctgaagtac ggggctgtgg gataaaaaca atctggagga
    37201 atgtcgtgca aacctttcaa gccgatcttg ccattgtagg cgccggtggc gcgggattac
    37261 gtgctgcaat tgctgccgcg caggcaaatc caaatgcaaa aatcgcacta atctcaaaag
    37321 tatacccgat gcgtagccat accgttgctg cagaaggggg ctccgccgct gtcgcgcagg
    37381 atcatgacag cttcgaatat cactttcacg atacagtagc gggtggcgac tggttgtgtg
    37441 agcaggatgt cgtggattat ttcgtccacc actgcccaac cgaaatgacc caactggaac
    37501 tgtgggggtg cccatggagc cgtcgcccgg atggtagcgt caacgtacgt cgcttcggcg
    37561 gcatgaaaat cgagcgcacc tggttcgccg ccgataagac cggcttccat atgctgcaca
    37621 cgctgttcca gacctctctg caattcccgc agatccagcg ttttgacgaa catttcgtgc
    37681 tggatattct ggttgatgat ggtcatgttc gcggcctggt agcaatgaac atgatggaag
    37741 gcacgctggt gcagatccgt gctaacgcgg tcgttatggc taccggcggt gcgggtcgcg
    37801 tttatcgtta caacaccaac ggcggcatcg ttaccggtga cggtatgggt atggcgctaa
    37861 gccacggcgt tccgctgcgt gacatggaat tcgttcagta tcacccaacc ggcctaccag
    37921 gttccggtat cctgatgacc gaaggctgcc gcggtgaagg tggtattctg gtcaacaaaa
    37981 atggctaccg ttatctgcaa gattacggca tgggcccgga aactccgctg ggcgagccga
    38041 aaaacaaata tatggaactg ggtccacgcg acaaagtttc tcaggccttc tggcacgaat
    38101 ggcgtaaagg caacaccatc tccacgccac gtggcgatgt ggtttacctc gacctgcgtc
    38161 accttggcga gaaaaaactg catgaacgtc tgccgttcat ctgcgaactg gcgaaagcgt
    38221 acgttggcgt cgatccggtt aaagaaccga ttccggtacg tccgaccgca cactacacca
    38281 tgggcggtat cgaaaccgat cagaactgtg aaacccgcat taaaggtctg ttcgccgtgg
    38341 gtgaatgttc ctctgttggt ctgcacggtg cgaaccgtct gggctccaac tcgctggcag
    38401 aactggtggt cttcggtcgt ctggccggtg aacaagcgac agagcgtgca gcaactgccg
    38461 gtaatggcaa cgaagcggca attgaagcgc aggcagctgg cgttgaacaa cgtctgaaag
    38521 atctggttaa ccaggatggc ggcgaaaact gggctaagat ccgcgacgaa atgggcatgg
    38581 ccatggaaga aggctgcggt atctaccgta cgccggaact gatgcagaaa accatcgaca
    38641 agctggcaga gctgcaggaa cgcttcaagc gcgtgcgcat caccgacact tccagcgtgt
    38701 tcaacaccga cctgctctac accattgagc tgggccacgg tctgaacgtt gctgaatgta
    38761 tggcgcactc cgcaatggca cgtaaagagt cccgcggcgc gcaccagcgt ctggacgaag
    38821 gttgcaccga gcgtgacgac gtcaacttcc tcaaacacac cctcgccttc cgcgatgctg
    38881 atggcacgac tcgcctggag tacagcgacg tgaagattac tacgctgccg ccagctaaac
    38941 gcgtttacgg tggcgaagcg gatgcagccg ataaggcgga agcagccaat aagaaggaga
    39001 aggcgaatgg ctgagatgaa aaacctgaag attgaggtgg tgcgctataa cccggaagtc
    39061 gataccgcac cgcatagcgc attctatgaa gtgccttatg acgcaactac ctcattactg
    39121 gatgcgctgg gctacatcaa agacaacctg gcaccggacc tgagctaccg ctggtcctgc
    39181 cgtatggcga tttgtggctc ctgcggcatg atggttaaca acgtgccaaa actggcatgt
    39241 aaaaccttcc tgcgtgatta caccgacggt atgaaggttg aagcgttagc taacttcccg
    39301 attgaacgcg atctggtggt cgatatgacc aacttcatcg aaagtctgga agcgatcaaa
    39361 ccgtacatca tcggcaactc ccgcaccgcg gatcagggta ctaacatcca gaccccggcg
    39421 cagatggcga agtatcacca gttctccggt tgcatcaact gtggtctgtg ctacgccgcg
    39481 tgcccgcagt ttggcctgaa cccagagttc atcggtccgg ctgccattac gctggcgcat
    39541 cgttataacg aagatagccg cgaccacggt aagaaggagc gtatggcgca gttgaacagc
    39601 cagaacggcg tatggagctg tactttcgtg ggctactgct ccgaagtctg cccgaaacac
    39661 gtcgatccgg ctgcggccat tcagcagggc aaagtagaaa gttcgaaaga ctttcttatc
    39721 gcgaccctga aaccacgcta aggagtgcaa catgacgact aaacgtaaac cgtatgtacg
    39781 gccaatgacg tccacctggt ggaaaaaatt gccgttttat cgcttttaca tgctgcgcga
    39841 aggcacggcg gttccggctg tgtggttcag cattgaactg attttcggcc tgtttgccct
    39901 gaaaaatggc ccggaagcct gggcgggatt cgtcgacttt ttacaaaacc cggttatcgt
    39961 gatcattaac ctgatcactc tggcggcagc cctgctgcac accaaaacct ggtttgagct
    40021 ggcaccaaaa gcagccaata tcattgtaaa agacgaaaaa atgggaccag agccaattat
    40081 caaaagtctc tgggcggtaa ctgtggttgc caccatcgta atcctgtttg ttgccctgta
    40141 ctggtaagga gcctgagatg attaatccaa atccaaagcg ttctgacgaa ccggtattct
    40201 ggggcctctt cggggccggt ggtatgtgga gcgccatcat tgcgccggtg atgatcctgc
    40261 tggttggtat tctgctgccg ctagggctgt ttccgggtga tgcgctgagc tacgagcgcg
    40321 ttctggcgtt cgcgcagagc ttcattggtc gcgtattcct gttcctgatg atcgttctgc
    40381 cgctgtggtg tggtttacac cgtatgcacc acgcgatgca cgatctgaaa atccacgtac
    40441 ctgcgggtaa atgggttttc tacggtctgg ctgctatcct gacagttgtc acactgattg
    40501 gtgtcgttac aatctaacgc atcgccaatg taaatctggc ccgcctatgg cgggccgttt
    40561 tgtatggaaa ccagacccta tgttcaaaac gacgctctgc accttattaa ttaccgcctc
    40621 ttgctccaca tttgccgcac ctcaacaaat caacgatatt gtgcatcgca caattacccc
    40681 gcttatagag caacaaaaga tccccggtat ggcggtagcg gtaatttatc agggtaaacc
    40741 ttattacttt acctggggct atgcggacat cgccaaaaag cagcccgtca cacagcaaac
    40801 gttgtttgag ttaggttcgg tcagcaaaac atttacgggc gtgcttggtg gcgacgctat
    40861 tgctcgaggg gaaatcaagt taagcgatcc cacaacaaaa tactggcctg aacttaccgc
    40921 taaacagtgg aatgggatca cactattaca tctcgcaacc tacaccgctg gcggcctgcc
    40981 attgcaggtg ccggatgagg tgaaatcctc aagcgacttg ctgcgcttct atcaaaactg
    41041 gcagcctgca tgggctccag gaacacaacg tctgtatgcc aactccagta tcggtttgtt
    41101 cggcgcactg gctgtgaagc cgtctggttt gagttttgag caggcgatga aaactcgtgt
    41161 cttccagcca ctcaaactca atcatacgtg gattaatgta ccgtccgcag aagaaaagaa
    41221 ctacgcctgg ggatatcgcg aaggtaaggc agtacatgtt tcgccagggg cgttagatgc
    41281 tgaagcttat ggtgtgaagt cgaccattga agatatggcc cgctgggtgc aaagcaattt
    41341 aaaacccctt gatatcaatg agaaaacact tcaacaaggg atacaactgg cacaatctcg
    41401 ctactggcaa accggcgata tgtatcaggg tctgggctgg gaaatgctgg actggccggt
    41461 aaatcctgac atcatcatta acggcagtga caataaaatt gcactggcag cacgccccgt
    41521 aaaaccgatt acgcccccaa ctcctgcagt acgcgcatca tgggtacata aaacgggggc
    41581 gaccggcgga tttggtagct atgtcgcgtt tattccagaa aaagagctgg gtatcgtgat
    41641 gctggctaac aaaaactacc ccaatccagc gagagtcgcc gccgcctggc agattctcaa
    41701 cgctctacag taaaatttca tcgggtccga attttcggac cttttctccg cttttccttg
    41761 ctgtcatcta cacttagaaa aaaaccagta aggaaacatt tatgcgcctg ctccctctcg
    41821 ttgccgcagc gacagctgca tttctggtcg ttgcctgcag ttcccctacg ccgccgcgtg
    41881 gcgtgaccgt agtgaataat ttcgacgcta aacgttatct cggtacctgg tatgagattg
    41941 cccgttttga tcaccgcttt gaacgtggac tggaaaaagt caccgcaaca tacagcctgc
    42001 gtgatgacgg cggcctgaat gtcattaata aaggctataa ccctgacaga gaaatgtggc
    42061 agcagagtga agggaaagcg tactttaccg gcgtaccaac tcgcgctgcg ctgaaagtgt
    42121 cattctttgg tcctttctat ggcggttata acgttattgc actcgatcgg gaataccgcc
    42181 atgcgctggt ttgcggcccg gaccgcgact acctgtggat actctcccgc acgccaacca
    42241 tttctgacga agtgaaacag gagatgctgg cagtcgcgac ccgggaaggg tttgatgtca
    42301 gtaagtttat ttgggtacag cagcccggta gttaatgagt gctgagtttc agaccaataa
    42361 tccccaatac gattaacgcc agactcgcca ggcgcatcgg gttagcggac tcaccgagca
    42421 gcacaatgcc ggtgatggcc gcgccgactg cgccaatacc cgtccacacg gcataagccg
    42481 tccctactgg taacgatttc atcgcccagg caagtagcgc cagactgaca atcatcgccg
    42541 tcacggtaat aacacttggc gtcaaacgac taaagccgtg agtatatttc aggccaacgg
    42601 cccataccac ttccagcaga ccagcaataa ctaagataat ccaggacata tcaggctccg
    42661 gaaaattggg gccgtccccg gtgaaaagac gcgtttgcag gtcgtcctgc aaagctgatg
    42721 gttgaacgga tattttgcca ggtggcaggg gaatttcaac tattacctat gtaactatcg
    42781 cagaaaaaac aaacggcacg acacagctgt cgtaccgtta ttgcttattg ctgcgctttc
    42841 gttgctgcgc cagaaatcgc gttaccgcca tcagaaatgt cttcaccaac gccacgcgtg
    42901 gtgttgcagg cagttaatac tgttgaaagc accagaacag aaaaaatcgc tgcaattgtc
    42961 ttcttcacca
//
LOCUS       DADSYM010000016        41618 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2415
            SAMN08039579-rid11723173.denovo.016, whole genome shotgun sequence.
ACCESSION   DADSYM010000016 DADSYM010000000
VERSION     DADSYM010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039579
            Sequence Read Archive: SRR6362533
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 41618)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 41618)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980162.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 03:39:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,484
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 4,347
            CDSs (with protein)               :: 4,347
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 43 of 85
            Pseudo Genes (incomplete)         :: 37 of 85
            Pseudo Genes (internal stop)      :: 19 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41618
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039579-rid11723173.denovo.016"
                     /strain="LGE2415"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8302 N 95.9628 W"
                     /collection_date="2015-08-24"
     gene            complement(41..949)
                     /gene="galU"
                     /locus_tag="J1Y83_000753"
     CDS             complement(41..949)
                     /gene="galU"
                     /locus_tag="J1Y83_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HBA7628933.1"
                     /translation="MAAINTKVKKAVIPVAGLGTRMLPATKAIPKEMLPLVDKPLIQY
                     VVNECIAAGITEIVLVTHSSKNSIENHFDTSFELEAMLEKRVKRQLLDEVQSICPPHV
                     TIMQVRQGLAKGLGHAVLCAHPVVGDEPVAVILPDVILDEYESDLSQDNLAEMIRRFD
                     ETGHSQIMVEPVADVTAYGVVDCKGVELAPGESVPMVGVVEKPKADVAPSNLAIVGRY
                     VLSADIWPLLAKTPPGAGDEIQLTDAIDMLIEKETVEAYHMKGKSHDCGNKLGYMQAF
                     VEYGIRHNTLGTEFKAWLEDEMGIKK"
     gene            complement(1151..2164)
                     /gene="rssB"
                     /locus_tag="J1Y83_000754"
     CDS             complement(1151..2164)
                     /gene="rssB"
                     /locus_tag="J1Y83_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RssB"
                     /protein_id="HBA7628934.1"
                     /translation="MTQPLVGKQILIVEDEQVFRSLLDSWFSSLGATTVLAADGVDAL
                     ELLGGFTPDLMICDIAMPRMNGLKLLEHIRNRGDQTPVLVISATENMADIAKALRLGV
                     EDVLLKPVKDLNRLREMVFACLYPSMFNSRVEEEERLFRDWDAMVDNPAAAAKLLQEL
                     QPPVQQVISQCRVNYRQLVAADKPGLVLDIAALSENDLAFYCLDVTRAGHNGVLAALL
                     LRALFNGLLQEQLAHQNQRLPELGALLKQVNHLLRQANLPGQFPLLVGYYHRELKNLI
                     LVSAGLNATLNTGEHQVQISNGVPLGTLGNAYLNQLSQRCDAWQCQIWGTGGRLRLML
                     SAE"
     gene            complement(2256..3161)
                     /gene="rssA"
                     /locus_tag="J1Y83_000755"
     CDS             complement(2256..3161)
                     /gene="rssA"
                     /locus_tag="J1Y83_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase RssA"
                     /protein_id="HBA7628935.1"
                     /translation="MRKIKIGLALGSGAARGWSHIGVINALKKVGIEIDIVAGCSIGS
                     LVGAAYACDRLSALEDWVTSFSYWDVLRLMDLSWQRGGLLRGERVFNQYREIMPETEI
                     ENCSRRFAAVATNLSTGRELWFTEGDLHLAIRASCSIPGLMAPVAHNGYWLVDGAVVN
                     PIPISLTRALGADIVIAVDLQHDAHLMQQDLLSFNVSEENSENGDSLPWHARLKERLG
                     SITTRRAVTAPTATEIMTTSIQVLENRLKRNRMAGDPPDILIQPVCPQISTLDFHRAH
                     AAIAAGQLAVEKKMDELLPLVRTNI"
     gene            3274..3732
                     /locus_tag="J1Y83_000756"
     CDS             3274..3732
                     /locus_tag="J1Y83_000756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YchJ family protein"
                     /protein_id="HBA7628936.1"
                     /translation="MSQLCPCGSAVEYSLCCHPYVSGEKVAPDPEHLMRSRYCAFVMQ
                     DADYLIKTWHPSCGAAALRAELIAGFAHTEWLGLTVFEHCWQDGGNIGFVSFVAQFTE
                     GGKTGAIIERSRFLKENGQWYYIDGTRPQFGRNDPCPCGSGKKFKKCCGQ"
     gene            3782..4624
                     /gene="purU"
                     /locus_tag="J1Y83_000757"
     CDS             3782..4624
                     /gene="purU"
                     /locus_tag="J1Y83_000757"
                     /EC_number="3.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008784958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formyltetrahydrofolate deformylase"
                     /protein_id="HBA7628937.1"
                     /translation="MHSLQRKVLRTICPDQKGLIARITNICYKHELNIVQNNEFVDHR
                     TGRFFMRTELEGIFNDSTLLADLDSALPEGSVRELNPAGRRRIVILVTKEAHCLGDLL
                     MKANYGGLDVEIAAVIGNHDTLRSLVERFDIPFELVSHEGLSRNEHDQKMADAIDAYQ
                     PDYVVLAKYMRVLTPEFVARFPNKIINIHHSFLPAFIGARPYHQAYERGVKIIGATAH
                     YVNDNLDEGPIIMQDVIHVDHTYTAEDMMRAGRDVEKNVLSRALYKVLAQRVFVYGNR
                     TIIL"
     gene            4784..4868
                     /locus_tag="J1Y83_000758"
     tRNA            4784..4868
                     /locus_tag="J1Y83_000758"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4818..4820,aa:Tyr,seq:gta)
     gene            4915..5046
                     /locus_tag="J1Y83_000759"
     ncRNA           4915..5046
                     /ncRNA_class="other"
                     /locus_tag="J1Y83_000759"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
     gene            complement(5231..5908)
                     /gene="narI"
                     /locus_tag="J1Y83_000760"
     CDS             complement(5231..5908)
                     /gene="narI"
                     /locus_tag="J1Y83_000760"
                     /EC_number="1.7.99.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="respiratory nitrate reductase subunit gamma"
                     /protein_id="HBA7628938.1"
                     /translation="MQFLNMFFFDIYPYIAGAVFLIGSWLRYDYGQYTWRAASSQMLD
                     RKGMNLASNLFHIGILGIFVGHFFGMLTPHWMYEAWLPIEVKQKMAMFAGGASGVLCL
                     IGGVLLLKRRLFSPRVRATTTGADILILSLLVIQCALGLLTIPFSAQHMDGSEMMKLV
                     GWAQSVVTFHGGASQHLDGVAFIFRLHLVLGMTLFLLFPFSRLVHIWSVPVEYLTRKY
                     QLVRARH"
     gene            complement(5908..6618)
                     /gene="narJ"
                     /locus_tag="J1Y83_000761"
     CDS             complement(5908..6618)
                     /gene="narJ"
                     /locus_tag="J1Y83_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase molybdenum cofactor assembly
                     chaperone"
                     /protein_id="HBA7628939.1"
                     /translation="MIELVIVSRLLEYPDAALWQHQQEMFEAIAASKNLPKEDAHALG
                     IFLRDLTTMDPLDAQAQYSELFDRGRATSLLLFEHVHGESRDRGQAMVDLLAQYEQHG
                     LQLNSRELPDHLPLYLEYLAQLPQSEAVEGLKDIAPILALLSARLQQRESRYAVLFDL
                     LLKLANTAIDSDKVAEKIADEARDDTPQALDAVWEEEQVKFFAEKGCGDSAITAHQRR
                     FAGAVAPQYLNITTGGQH"
     gene            complement(6615..8153)
                     /gene="narH"
                     /locus_tag="J1Y83_000762"
     CDS             complement(6615..8153)
                     /gene="narH"
                     /locus_tag="J1Y83_000762"
                     /EC_number="1.7.99.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit beta"
                     /protein_id="HBA7628940.1"
                     /translation="MKIRSQVGMVLNLDKCIGCHTCSVTCKNVWTSREGVEYAWFNNV
                     ETKPGQGFPTDWENQEKYKGGWIRKINGKLQPRMGNRAMLLGKIFANPHLPGIDDYYE
                     PFDFDYQNLHTAPEGSKSQPVARPRSLITGERMAKIEKGPNWEDDLGGEFDKLAKDKN
                     FDNIQKAMYSQFENTFMMYLPRLCEHCLNPACVATCPSGAIYKREEDGIVLIDQDKCR
                     GWRMCITGCPYKKIYFNWKSGKSEKCIFCYPRIEAGQPTICSETCVGRIRYLGVLLYD
                     ADAIERAASTENEKDLYQRQLDVFLDPNDPKVIEQAIKDGIPLSVIEAAQQSPVYKMA
                     MEWKLALPLHPEYRTLPMVWYVPPLSPIQSAADAGELGSNGILPDVESLRIPVQYLAN
                     LLTAGDTKPVLRALKRMLAMRHYKRAETVDGKVDTRALEEVGLTEAQAQEMYRYLAIA
                     NYEDRFVVPSSHRELAREAFPEKNGCGFTFGDGCHGSDTKFNLFNSRRIDAIDVTSKT
                     EPHP"
     gene            complement(8150..11893)
                     /locus_tag="J1Y83_000763"
     CDS             complement(8150..11893)
                     /locus_tag="J1Y83_000763"
                     /EC_number="1.7.99.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit alpha"
                     /protein_id="HBA7628941.1"
                     /translation="MSKFLDRFRYFKQKGETFADGHGQLLNTNRDWEDGYRQRWQHDK
                     IVRSTHGVNCTGSCSWKIYVKNGLVTWETQQTDYPRTRPDLPNHEPRGCPRGASYSWY
                     LYSANRLKYPMMRKRLMKMWREAKALHSDPVEAWASIIEDADKAKSFKQARGRGGFVR
                     SSWQEVNELIAASNVYTIKNYGPDRVAGFSPIPAMSMVSYASGARYLSLIGGTCLSFY
                     DWYCDLPPASPQTWGEQTDVPESADWYNSSYIIAWGSNVPQTRTPDAHFFTEVRYKGT
                     KTVAVTPDYAEIAKLCDLWLAPKQGTDAAMALAMGHVMLREFHLDNPSQYFTDYVRRY
                     TDMPMLVMLEERDGYYAAGRMLRAADLVDALGQENNPEWKTVAFNTNGEMVAPNGSIG
                     FRWGEKGKWNLEQRDGKTGEETELQLSLLGSQDEIAEVGFPYFGGDGTEHFNKVELEN
                     VLLHKLPVKRLQLADGSTALVTTVYDLTLANYGLERGLNDVNCATSYDDVKAYTPAWA
                     EQITGVSRSQIIRIAREFADNADKTHGRSMIIVGAGLNHWYHLDMNYRGLINMLIFCG
                     CVGQSGGGWAHYVGQEKLRPQTGWQPLAFALDWQRPARHMNSTSYFYNHSSQWRYETV
                     TAEELLSPMADKSRYTGHLIDFNVRAERMGWLPSAPQLGTNPLTIAREAEKAGMNPVD
                     YTVKSLKEGSIRFAAEQPENGKNHPRNLFIWRSNLLGSSGKGHEFMLKYLLGTEHGIQ
                     GKDLGQQGGVKPEEVDWQDNGLEGKLDLVVTLDFRLSSTCLYSDIILPTATWYEKDDM
                     NTSDMHPFIHPLSAAVDPAWEAKSDWEIYKAIAKKFSEVCVGHLGKETDIVTLPIQHD
                     SAAELAQPLDVKDWKKGECDLIPGKTAPHIMVVERDYPATYERFTSIGPLMEKIGNGG
                     KGIAWNTQSEMDLLRKLNYTKAEGPAKGQPMLNTAIDAAEMILTLAPETNGQVAVKAW
                     AALSEFTGRDHTHLALNKEDEKIRFRDIQAQPRKIISSPTWSGLEDEHVSYNAGYTNV
                     HELIPWRTLSGRQQLYQDHQWMRDFGESLLVYRPPIDTRSVKEVMGQKSNGNPEKALN
                     FLTPHQKWGIHSTYSDNLLMLTLGRGGPVVWLSEADAKELGIADNDWIEVFNSNGALT
                     ARAVVSQRVPAGMTMMYHAQERIVNLPGSEITQQRGGIHNSVTRITPKPTHMIGGYAH
                     LAYGFNYYGTVGSNRDEFVVVRKMKNIDWLDGEGNDQVQESVK"
     gene            complement(12286..13677)
                     /gene="narK"
                     /locus_tag="J1Y83_000764"
     CDS             complement(12286..13677)
                     /gene="narK"
                     /locus_tag="J1Y83_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate transporter NarK"
                     /protein_id="HBA7628942.1"
                     /translation="MSHSSAPERATGAVITDWRPEDPAFWQQRGQRIASRNLWISVPC
                     LLLAFCVWMLFSAVAVNLPKVGFNFTTDQLFMLTALPSVSGALLRVPYSFMVPIFGGR
                     RWTAFSTGILIIPCVWLGFAVQDTSTPYSVFIIISLLCGFAGANFASSMANISFFFPK
                     QKQGGALGLNGGLGNMGVSVMQLVAPLVVSLSIFAVFGSQGVKQPDGTELYLANASWI
                     WVPFLAIFTIAAWFGMNDLATSKASIKEQLPVLKRGHLWIMSLLYLATFGSFIGFSAG
                     FAMLSKTQFPDIQILQYAFFGPFIGALARSAGGALSDRLGGTRVTLVNFILMAIFSGL
                     LFLTLPTDGQGGSFMAFFAVFLALFLTAGLGSGSTFQMISVIFRKLTMDRVKAEGGSD
                     ERAMREAATDTAAALGFISAIGAIGGFFIPKAFGSSLALTGSPVGAMKVFLIFYIACV
                     VITWAVYGRHSKK"
     gene            14016..15812
                     /gene="narX"
                     /locus_tag="J1Y83_000765"
     CDS             14016..15812
                     /gene="narX"
                     /locus_tag="J1Y83_000765"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite two-component system sensor
                     histidine kinase NarX"
                     /protein_id="HBA7628943.1"
                     /translation="MLKRCLSPLTLVNQVALIVLLSTAIGLAGMAVSGWLVQGVQGSA
                     HAINKAGSLRMQSYRLLAAVPLSEKDKPLIKEMEQTAFSAELTRAAERDGQLAQLQGL
                     QDYWRNELIPALIRAQNRETVSADVSQFVAGLDQLVSGFDRTTEMRIETVVLVHRVMA
                     VFMALLLVFTIIWLRARLLQPWRQLLAMASAVSHRDFTQRANTSGRNEMAMLGTALNN
                     MSAELAESYAVLEQRVQEKTAGLEHKNQILSFLWQANRRLHSRAPLCERLSPVLNGLQ
                     NLTLLRDIELRVYDTDDEENHQEFTCQPDMTCDDKGCQLCPRGVLPVGDRGTTLKWRL
                     ADTHTQYGILLATLPQGRHLSHDQQQLVDTLVEQLTATLALDRHQERQQQLIVMEERA
                     TIARELHDSIAQSLSCMKMQVSCLQMQGDALPESSRELLSQIRNELNASWVQLRELLT
                     TFRLQLTEPGLRPALEASCEEYSAKFGFPVKLDYQLPPRLVPSHQAIHLLQIAREALS
                     NALKHSQASEVVVTVAQNDNQVKLTVQDNGCGVPENAIRSNHYGMIIMRDRAQSLRGD
                     CRVRRRESGGTEVVVTFIPEKTFTDVQGDTHE"
     gene            15805..16455
                     /gene="narL"
                     /locus_tag="J1Y83_000766"
     CDS             15805..16455
                     /gene="narL"
                     /locus_tag="J1Y83_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator NarL"
                     /protein_id="HBA7628944.1"
                     /translation="MSNQEPATILLIDDHPMLRTGVKQLISMAPDITVVGEASNGEQG
                     IELAESLDPDLILLDLNMPGMNGLETLDKLREKSLSGRIVVFSVSNHEEDVVTALKRG
                     ADGYLLKDMEPEDLLKALHQAAAGEMVLSEALTPVLAASLRANRATTERDVNQLTPRE
                     RDILKLIAQGLPNKMIARRLDITESTVKVHVKHMLKKMKLKSRVEAAVWVHQERIF"
     gene            complement(16456..17850)
                     /gene="ychO"
                     /locus_tag="J1Y83_000767"
     CDS             complement(16456..17850)
                     /gene="ychO"
                     /locus_tag="J1Y83_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inverse autotransporter invasin YchO"
                     /protein_id="HBA7628945.1"
                     /translation="MSRFIPRIIPFYLLLLAAGGTANAQSTFEQKAANPFDNNNDGLP
                     DLGMAPKNHDGEKHFAEIVKDFGETSMNDNGLDTGEQAKAFALGKVRDALSQQVNQHV
                     ESWLSPWGNASVDVKVDNEGHFTGSRGSWFVPLQDNDRYLTWSQLGLTQQDDGLVSNV
                     GVGQRWARGSWLVGYNTFYDNLLDENLQRAGFGAEAWGEYLRLSANFYQPFAAWHEQT
                     ATQEQRMARGYDLTARMRMPFYQHLNTSVSVEQYFGDRVDLFNSGTGYHNPVALSLGL
                     NYTPVPLVTVTAQHKQGESGENQNNLGLNLNYRFGVPLKKQLSAGEVAESQSLRGSRY
                     DNPQRNNLPTLEYRQRKTLTVFLATPPWDLKPGETVPLKLQIRSRYGIRQLIWQGDTQ
                     ILSLTPGAQANSEEGWTLIMPDWQNGEGASNHWRLSVVVEDNQGQRVSSNEITLTLVE
                     PFDALSNDELRWEP"
     gene            18035..18388
                     /gene="ychN"
                     /locus_tag="J1Y83_000768"
     CDS             18035..18388
                     /gene="ychN"
                     /locus_tag="J1Y83_000768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE/F sulfur relay family protein YchN"
                     /protein_id="HBA7628946.1"
                     /translation="MQKIVIVANGAPYGSESLFNSLRLAIALREQENNLDLRLFLMSD
                     AVTAGLRGQKPGEGYNIQQMLEILTAQNVPVKLCKTCTDGRGISTLPLIDGVEIGTLV
                     ELAQWTLSADKVLTF"
     gene            complement(18432..19127)
                     /locus_tag="J1Y83_000769"
     CDS             complement(18432..19127)
                     /locus_tag="J1Y83_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcyclotransferase"
                     /protein_id="HBA7628947.1"
                     /translation="MITRDFLMNADCKTAFGAIEESLLWSAEQRAASLAATLACRPDE
                     GPVWIFGYGSLMWNPALEFTESCTGTLVGWHRAFCLRLTAGRGTAHQPGRMLALKEGG
                     RTTGVAYRLPEETLEQELTLLWKREMITGCYLPTWCQLDLDDGRTVNAIVFIMDPRHP
                     EYESDTRAQVIAPLIAAASGPLGTNAQYLFSLEQELIKLGMQDDGLNDLLVSVKKLLA
                     ENYPDGVLRPGFA"
     gene            complement(19273..19503)
                     /gene="chaB"
                     /locus_tag="J1Y83_000770"
     CDS             complement(19273..19503)
                     /gene="chaB"
                     /locus_tag="J1Y83_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative cation transport regulator ChaB"
                     /protein_id="HBA7628948.1"
                     /translation="MPYKTKSDLPESVKHVLPSHAQDIYKEAFNSAWDQYKDKEDRRD
                     DASREETAHKVAWAAVKHEYVKGDDDKWHKKS"
     gene            19773..20873
                     /gene="chaA"
                     /locus_tag="J1Y83_000771"
     CDS             19773..20873
                     /gene="chaA"
                     /locus_tag="J1Y83_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-potassium/proton antiporter ChaA"
                     /protein_id="HBA7628949.1"
                     /translation="MSNAQEAVKTRHKETSLIFPVLALVVLFLWGSSQTLPVVIAINL
                     LALIGILSSAFSVVRHADVLAHRLGEPYGSLILSLSVVILEVSLISALMATGDAAPTL
                     MRDTLYSIIMIVTGGLVGFSLLLGGRKFATQYMNLFGIKQYLIALFPLAIIVLVFPMA
                     LPAANFSTGQALLVALISAAMYGVFLLIQTKTHQSLFVYEHEDDSDDDDPHHGKPSAH
                     SSLWHAIWLIVHLIAVIAVTKMNASPLETLLDSMNAPVAFTGFLVALLILSPEGLGAL
                     KAVLNNQVQRAMNLFFGSVLATISLTVPVVTLIAFMTGNELQFALGAPEMVVMVASLV
                     LCHISFSTGRTNVLNGAAHLALFAAYLMTIFA"
     gene            21279..21386
                     /locus_tag="J1Y83_000772"
     CDS             21279..21386
                     /locus_tag="J1Y83_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small toxic polypeptide LdrA/LdrC"
                     /protein_id="HBA7628950.1"
                     /translation="MTLAQFAMIFWHDLAAPILAGIITAAIVGLWRNRK"
     gene            complement(21534..22388)
                     /gene="kdsA"
                     /locus_tag="J1Y83_000773"
     CDS             complement(21534..22388)
                     /gene="kdsA"
                     /locus_tag="J1Y83_000773"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HBA7628951.1"
                     /translation="MKQKVVSIGDINVANDLPFVLFGGMNVLESRDLAMRICEHYVTV
                     TQKLGIPYVFKASFDKANRSSIHSYRGPGLEEGMKIFQELKQTFGVKIITDVHEPSQA
                     QPVADVVDVIQLPAFLARQTDLVEAMAKTGAVINVKKPQFVSPGQMGNIVDKFKEGGN
                     EKVILCDRGANFGYDNLVVDMLGFSIMKKVSGNSPVIFDVTHALQCRDPFGAASGGRR
                     AQVAELARAGMAVGLAGLFIEAHPDPEHAKCDGPSALPLAKLEPFLKQMKAIDDLVKG
                     FEELDTSK"
     gene            complement(22424..23233)
                     /locus_tag="J1Y83_000774"
     CDS             complement(22424..23233)
                     /locus_tag="J1Y83_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat-containing protein"
                     /protein_id="HBA7628952.1"
                     /translation="MRSLADFEFNKAPLCEGMILACEAIRRDFPSQDVYDELERLVSL
                     AKEEISQLLPLEEQLEKLIALFYGEWGFKASRGVYRLSDALWLDQVLKNRQGSAVSLG
                     AVLLWVANRLDLPLLPVIFPTQLILRIECPDGEIWLINPFNGESLSEHMLDVWLKGNI
                     SPSAELFYEDLDEADNIEVIRKLLDTLKASLMEENQMELALRTSEALLQFNPEDPYEI
                     RDRGLIYAQLDCEHVALNDLSYFVEQCPEDPISEMIRAQINNIAHKHIVLH"
     gene            complement(23237..23629)
                     /locus_tag="J1Y83_000775"
     CDS             complement(23237..23629)
                     /locus_tag="J1Y83_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SirB family protein"
                     /protein_id="HBA7628953.1"
                     /translation="MTSFSTLLSVHLISIALSVGLLTLRFWLRYQKHPQAFARWTRIV
                     PPVVDTLLLLSGIALMAKAHILPFSGQAQWLTEKLFGVIIYIVLGFIALDYRRMHSQQ
                     ARIIAFPLALVVLYIIIKLATTKVPLLG"
     gene            complement(23626..24459)
                     /gene="prmC"
                     /locus_tag="J1Y83_000776"
     CDS             complement(23626..24459)
                     /gene="prmC"
                     /locus_tag="J1Y83_000776"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HBA7628954.1"
                     /translation="MEYQHWLREAISQLQASESPRRDAEILLAHVTGKGRTFILAFGE
                     TQLTDEQCQQLDALLTRRRDGEPIAHLTGVREFWSLPLFVSPATLIPRPDTECLVEQA
                     LARLPEQPCRILDLGTGTGAIALALASERPDCEITAVDRMPDAVSLAQRNAQNLAIKN
                     IHILQSDWFSALAGQQFAMIVSNPPYIDEQDPHLQQGDVRFEPLTALVAADSGMADIV
                     HIIEQSRNALVSGGFLLLEHGWQQGEAVRQAFIHAGYHDVETCRDYGDNERVTLGRYY
                     Q"
     gene            complement(24459..25541)
                     /gene="prfA"
                     /locus_tag="J1Y83_000777"
     CDS             complement(24459..25541)
                     /gene="prfA"
                     /locus_tag="J1Y83_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012132158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HBA7628955.1"
                     /translation="MKPSIVAKLEALHERHEEVQALLGDAQTIADQERFRALSREYAQ
                     LSDVSRCFTDWQQVQEDIETAQMMLDDPEMREMAQDELREAKEKSEQLEQQLQVLLLP
                     KDPDDERNAFLEVRAGTGGDEAALFAGDLFRMYSRYAEARRWRVEIMSASEGEHGGYK
                     EIIAKISGDGVYGRLKFESGGHRVQRVPATESQGRIHTSACTVAVMPELPDAELPDIN
                     PADLRIDTFRSSGAGGQHVNTTDSAIRITHLPTGIVVECQDERSQHKNKAKALSVLGA
                     RIHAAEMAKRQQAEASTRRNLLGSGDRSDRNRTYNFPQGRVTDHRINLTLYRLDEVME
                     GKLDMLIEPIIQEHQADQLAALSEQE"
     gene            complement(25583..26839)
                     /gene="hemA"
                     /locus_tag="J1Y83_000778"
     CDS             complement(25583..26839)
                     /gene="hemA"
                     /locus_tag="J1Y83_000778"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HBA7628956.1"
                     /translation="MTLLALGINHKTAPVSLRERVSFSPDKLDQALDSLLAQPMVQGG
                     VVLSTCNRTELYLSVEEQDNLQEALIRWLCDYHNLNEEDLRKSLYWHQDNDAVSHLMR
                     VASGLDSLVLGEPQILGQVKKAFADSQKGHMKASELERMFQKSFSVAKRVRTETDIGA
                     SAVSVAFAACTLARQIFESLSTVTVLLVGAGETIELVARHLREHKVQKMIIANRTRER
                     AQILADEVGAEVIALSDIDERLREADIIISSTASPLPIIGKGMVERALKSRRNQPMLL
                     VDIAVPRDVEPEVGKLANAYLYSVDDLQSIISHNLAQRKAAAVEAETIVAQETSEFMA
                     WLRAQSASETIREYRSQAEHVRDELTAKALAALEQGGDAQAIMQDLAWKLTNRLIHAP
                     TKSLQQAARDGDNERLNILRDSLGLE"
     gene            27053..27676
                     /gene="lolB"
                     /locus_tag="J1Y83_000779"
     CDS             27053..27676
                     /gene="lolB"
                     /locus_tag="J1Y83_000779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein localization protein LolB"
                     /protein_id="HBA7628957.1"
                     /translation="MPLPDFRLIRLLPLAALVLTACSVTTPKGPGKSPDSPQWRQHQQ
                     DVRNLNQYQTRGAFAYISDQQKVYARFFWQQTGQDRYRLLLTNPLGSTELELNAQPGN
                     VQLVDNKGQRYTSDDAEEMIGKLTGMPIPLNSLRQWILGLPGDATDYKLDDQYRLSEI
                     TYSQNGKNWKVVYGGYDTKTQPAMPANMELTDGGQRIKLKMDNWIVK"
     gene            27676..28527
                     /gene="ispE"
                     /locus_tag="J1Y83_000780"
     CDS             27676..28527
                     /gene="ispE"
                     /locus_tag="J1Y83_000780"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HBA7628958.1"
                     /translation="MRTQWPSPAKLNLFLYITGQRADGYHTLQTLFQFLDYGDTISIE
                     LRDDGDIRLLTPVEGVEHEDNLIVRAARLLMKTAANSGRLSTGSGANISIDKRLPMGG
                     GLGGGSSNAATVLVALNHLWQCGLSMDELAEMGLTLGADVPVFVRGHAAFAEGVGEIL
                     TPVDPPEKWYLVAHPGVSIPTPVIFKDPELPRNTPKRSIETLLKCEFSNDCEVIARKR
                     FREVDAVLSWLLEYAPSRLTGTGACVFAEFDTESEARQVLEQAPEWLNGFVAKGVNLS
                     PLHRAML"
     gene            28678..29625
                     /locus_tag="J1Y83_000781"
     CDS             28678..29625
                     /locus_tag="J1Y83_000781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HBA7628959.1"
                     /translation="MPDMKLFAGNATPELAQRIANRLYTSLGDAAVGRFSDGEVSVQI
                     NENVRGGDIFIIQSTCAPTNDNLMELVVMVDALRRASAGRITAVIPYFGYARQDRRVR
                     SARVPITAKVVADFLSSVGVDRVLTVDLHAEQIQGFFDVPVDNVFGSPILLEDMLQLN
                     LDNPIVVSPDIGGVVRARAIAKLLNDTDMAIIDKRRPRANVSQVMHIIGDVAGRDCVL
                     VDDMIDTGGTLCKAAEALKERGAKRVFAYATHPIFSGNAANNLRNSVIDEVVVCDTIP
                     LSDEIKSLPNVRTLTLSGMLAEAIRRISNEESISAMFEH"
     gene            29750..31429
                     /gene="dauA"
                     /locus_tag="J1Y83_000782"
     CDS             29750..31429
                     /gene="dauA"
                     /locus_tag="J1Y83_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylic acid transporter DauA"
                     /protein_id="HBA7628960.1"
                     /translation="MNKIFSSHVMPFRALIDACWKEKYTAARFTRDLIAGITVGIIAI
                     PLAMALAIGSGVAPQYGLYTAAVAGIVIALTGGSRFSVSGPTAAFVVILYPVSQQFGL
                     AGLLVATLLSGIFLILMGLARFGRLIEYIPVSVTLGFTSGIGITIGTMQIKDFLGLQM
                     AHVPEHYLQKVGALFMALPTINVGDAAIGIVTLGILVFWPRLGIRLPGHLPALLAGCA
                     VMGIVNLLGRHVATIGSQFHYVLADGSQGNGIPQLLPQLVLPWDLPNSEFTLTWDSIR
                     TLLPAAFSMAMLGAIESLLCAVVLDGMTGTKHKANSELVGQGLGNIIAPFFGGITATA
                     AIARSAANVRAGATSPISAVIHSILVILALLVLAPLLSWLPLSAMAALLLMVAWNMSE
                     AHKVVDLLRHAPKDDIIVMLLCMSLTVLFDMVIAISVGIVLASLLFMRRIARMTRLAP
                     VVVDVPDDVLVLRVIGPLFFAAAEGLFTDLESRLEGKRIVILKWDAVPVLDAGGLDAF
                     QRFVKRLPEGCELRVCNLEFQPLRTMARAGIQPIPGRLAFFPNRRAAMADL"
     gene            complement(31484..31762)
                     /gene="ychH"
                     /locus_tag="J1Y83_000783"
     CDS             complement(31484..31762)
                     /gene="ychH"
                     /locus_tag="J1Y83_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress-induced protein YchH"
                     /protein_id="HBA7628961.1"
                     /translation="MKRKNASLLGNVLMGLGLVVMVVGVGYSILNQLPQFNMPQYFAH
                     GAVLSIFVGAILWLAGARVGGHEQVCDRYWWVRHYDKRCRRSDNRRHS"
     gene            32040..32624
                     /gene="pth"
                     /locus_tag="J1Y83_000784"
     CDS             32040..32624
                     /gene="pth"
                     /locus_tag="J1Y83_000784"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HBA7628962.1"
                     /translation="MTIKLIVGLANPGAEYAATRHNAGAWFVDLLAERLRAPLREEAK
                     FFGYTSRVTLGGEDVRLLVPTTFMNLSGKAVAAMASFFRINPDEILVAHDELDLPPGV
                     AKFKLGGGHGGHNGLKDIISKLGNNPNFHRLRIGIGHPGDKNKVVGFVLGKPPVSEQK
                     LIDEAIDEAARCTEMWFTDGLTKATNRLHAFKAQ"
     gene            32741..33832
                     /gene="ychF"
                     /locus_tag="J1Y83_000785"
     CDS             32741..33832
                     /gene="ychF"
                     /locus_tag="J1Y83_000785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001538644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HBA7628963.1"
                     /translation="MGFKCGIVGLPNVGKSTLFNALTKAGIEAANFPFCTIEPNTGVV
                     PMPDPRLDQLAEIVKPQRTLPTTMEFVDIAGLVKGASKGEGLGNQFLTNIRETEAIGH
                     VVRCFENDNIIHVSGKVNPADDIEVINTELALADLDTCERAIHRVQKKAKGGDKDAKA
                     ELAVLEKCLPQLENAGMLRALDLSAEEKAAVRYLSFLTLKPTMYIANVNEDGFENNPY
                     LDQVREIAAKEGSVVVPVCAAVEADIAELDDEERDEFMQELGLEEPGLNRVIRAGYKL
                     LNLQTYFTAGVKEVRAWTIPVGATAPQAAGKIHTDFEKGFIRAQTISFEDFITYKGEQ
                     GAKEAGKMRAEGKDYIVKDGDVMNFLFNV"
     gene            33970..34200
                     /locus_tag="J1Y83_000786"
     CDS             33970..34200
                     /locus_tag="J1Y83_000786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001346938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA7628964.1"
                     /translation="MRLLCDNNHSALTVQEFVMKKTFTFSLLAIVFASLLSACVPHHH
                     HRHSDGPRGPAPSGKPMPPSNGGPNHGSHGRY"
     gene            complement(34237..36165)
                     /gene="dhaR"
                     /locus_tag="J1Y83_000787"
     CDS             complement(34237..36165)
                     /gene="dhaR"
                     /locus_tag="J1Y83_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001205426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS-dependent dihydroxyacetone kinase operon
                     transcriptional regulator DhaR"
                     /protein_id="HBA7628965.1"
                     /translation="MRDMSGAFNNDDRGISPLIATSWERCNKLMKRETWNVPHQAQGV
                     TFASIYRRKKAMLTLGQAALEDAWEYMAPRECALLILDETACILSRNGDPQTLQQLSV
                     LGFNDGTYCAEGIIGTCALSLAAISGQAVKTMADQHFKQALWNWAFCATPLFDSKGRL
                     TGTIALACPVEQTTAADLPLTLAIAREVGNLLLTDSLLAETNRHLNQLNALLESMDDG
                     VISWDEQGNLQFINAQAARVLRLDATASQGRAITELLTLPAVLQQAIKQAHPLKHVEA
                     TFESQHQFIDAVITLKPIIETQGTSFILLLHPVEQMRQLMTSQLGKVSHTFAHMPQDD
                     PQTRRLIHFGRQAARSSFPVLLCGEEGVGKALLSQAIHNESERAAGPYIAVNCELYGD
                     AALAEEFIGGDRTDSENGRLSRLELAHGGTLFLEKIEYLAVELQSALLQVIKQGVITR
                     LDARRLIPIDVKVIATTTADLAMLVEQNRFSRQLYYALHAFEITIPPLRMRRGSIPAL
                     VNNKLRSLEKRFSTRLKIDDDALARLVSCAWPGNDFELYSVIENLALSSDNGRIRVSD
                     LPEHLFTEQATDDVSATRLSTSLSFAEVEKEAIINAAQVTGGRIQEMSALLGIGRTTL
                     WRKMKQHGIDAGQFKRRG"
     gene            36384..37454
                     /gene="dhaK"
                     /locus_tag="J1Y83_000788"
     CDS             36384..37454
                     /gene="dhaK"
                     /locus_tag="J1Y83_000788"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309732.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HBA7628966.1"
                     /translation="MKKLINDVQDVLDEQLAGLAKAHPSLTLHQDPVYVTRADAPVAG
                     KVALLSGGGSGHEPMHCGYIGQGMLSGACPGEIFTSPTPDKIFECAMQIDGGEGVLLI
                     IKNYTGDILNFETATELLHDSGVKVTTVVIDDDVAVKDSLYTAGRRGVANTVLIEKLV
                     GAAAERGDSLDACAELGRKLNNQGHSIGIALGACTVPAAGKPSFTLADNEMEFGVGIH
                     GEPGIDRRPFSSLDQTVDEMFDTLLENGSYHRTLRFWDYQQGSWQEEPQTKQPLQSGD
                     RVIALVNNLGATPLSELYGVYNRLTTRCQQAGLTIERNLIGAYCTSLDMTGFSITLLK
                     VDDETLALWDAPVHTPALNWGK"
     gene            37465..38097
                     /gene="dhaL"
                     /locus_tag="J1Y83_000789"
     CDS             37465..38097
                     /gene="dhaL"
                     /locus_tag="J1Y83_000789"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase ADP-binding subunit
                     DhaL"
                     /protein_id="HBA7628967.1"
                     /translation="MSLSRTQIVNWLTRCGDIFSTESEYLTGLDREIGDADHGLNMNR
                     GFSKVVEKLPAIADKDIGFILKNTGMTLLSSVGGASGPLFGTFFIRAAQTTQARQSLT
                     LEELYQMFRDGADGVISRGKAEPGDKTMCDVWVPVVESLRQSCKQNLSVPAALDAASS
                     IAESAAQSTITMQARKGRASYLGERSIGHQDPGATSVMFMMQMLALAAKE"
     gene            38108..39526
                     /gene="dhaM"
                     /locus_tag="J1Y83_000790"
     CDS             38108..39526
                     /gene="dhaM"
                     /locus_tag="J1Y83_000790"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415716.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaM"
                     /protein_id="HBA7628968.1"
                     /translation="MVNLVIVSHSSRLGEGVGELARQMLMSDNCKIAIAAGIDDPQNP
                     IGTDAVKVMEAIESVADADHVLVMMDMGSALLSAETALELLAPEIAAKVRLCAAPLVE
                     GTLAATVSAASGADIDKVIFDAMHALEAKREQLGLPSSDTEISDTCPPYDEEARSLSV
                     VIKNRNGLHVRPASRLVYTLSTFNADMLLEKNGKCVTPESINQIALLQVRYNDTLRLI
                     AKGPEAEEALIAFRQLAEDNFGETEEVAPPTLRPVPPVSGKAFYYQPVLCTVQAKSTL
                     TVEEEQERLRQAIDFTLLDLMTLTAKAETCGLDDIAAIFSGHHTLLDDPELQAAASEL
                     LQHEHCTAEYAWQQVLKELSQQYQQLDDEYLQARYIDVDDLLHRTLVHLTQTKEELPQ
                     FNSPTILLAENIYPSTVLQLDPAVVKGICLSAGSPLSHSALIARELGIGWICQQGEKL
                     YAIQPEETLTLDVKTQRFNRQG"
     gene            39846..41543
                     /locus_tag="J1Y83_000791"
     CDS             39846..41543
                     /locus_tag="J1Y83_000791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalase"
                     /protein_id="HBA7628969.1"
                     /translation="MKSPAPSRPQKMALIPACIFLCFAALSVQAEETSVTPQPPDILL
                     GPLFNDVQNAKLFPDQKTFADAVPNSDPLMILADYRMQQNQSGFDLRHFVNVNFTLPK
                     EGEKYVPPEGQSLREHIDGLWLVLTRSTENTEKWDSLLPLPKPYVVPGGRFREVYYWD
                     SYFTMLGLAESGHWDKVADMVANFAHEIDNYGHIPNGNRSYYLSRSQPPFFALMVELL
                     AQHEGDAALKQYLPQMQKEYAYWMDGVENLQAGQQEKRVVKLQDGTLLNRYWDDRDTP
                     RPESWVEDIATAKSNPNRPATEIYRDLRSAAASGWDFSSRWMDNPQQLNTLRTTSIVP
                     VDLNSLMFKMEKILARASKAIGDNAMANQYETLANARQKGIEKYLWNDQQGWYADYDL
                     KSHKVRNQLTAAALFPLYVNAAAKDRASKMATATKTHLLQPGGLNTTSVKSGQQWDAP
                     NGWAPLQWVATEGLQNYGQKEVAMDISWHFLTNVQHTYDREKKLVEKYDVSTTGTGGG
                     GGEYPLQDGFGWTNGVTLKMLDLICPKEQPCDNVPATRPLSESTTQPLKQKEAEPTP"
BASE COUNT        10203 a        10924 c        10305 g        10186 t
ORIGIN      
        1 attgctcaac gccgtttcgt ggaaaacacc gatacggatg ttacttctta atgcccatct
       61 cgtcttcaag ccaggcttta aattccgtgc caagggtgtt atgacgaata ccgtattcaa
      121 cgaaggcctg catgtaacct aatttattac cgcagtcatg gctcttccct ttcatatgat
      181 aggcttcaac cgtttctttt tcgatcagca tatcaattgc gtcggtgagc tgaatttcat
      241 caccagctcc aggaggggtt tttgccagca acggccaaat atccgcgcta agtacgtaac
      301 gacccacaat agcgagatta gacggcgcaa catccgcttt tggtttttca accacgccaa
      361 ccatcggtac gctttcaccc ggcgctaatt caacgccttt gcaatccaca acgccatatg
      421 cggtcacatc agcaaccggt tcaaccatga tctggctatg acccgtttca tcaaagcggc
      481 ggatcatctc tgccaggtta tcctgtgata aatcggattc atattcatcc agaataacgt
      541 caggcaaaat aacagctacc ggttcatcac ccactaccgg gtgagcacac aataccgcgt
      601 gtcccaggcc tttcgccagc ccctgacgaa cttgcataat agtcacgtgt ggcggacaaa
      661 tagactgcac ttcatcaagc agttgacgtt ttacacgttt ttccagcatt gcttccagtt
      721 caaaactggt atcaaagtgg ttttcaatag agtttttaga tgagtgagta accagcacaa
      781 tttcagtaat gccagccgca atacattcat tcacgacgta ttgaattaat ggcttatcga
      841 caagtggcag catctctttc gggatggctt tcgtcgccgg caacatcctg gttcctaatc
      901 ccgctacggg gataacggct tttttgactt tcgtattaat ggcagccatt taaattctcc
      961 tggactgttc gtgttttgaa cgtgttcata tttctgtatc gcatcccagt atatcagcac
     1021 cccgaagggg ctaagttctg aaaaggaggc gagttagcat aattaagaca attacttata
     1081 cgaatagcga cgatagtacc accagccgac attagcaggt aatgcaaatt tagcccgtgt
     1141 tatcgtttgc tcattctgca gacaacatca agcgcagccg accaccggtt ccccatattt
     1201 ggcattgcca ggcatcgcat cgctggctca attgattcaa ataagcgtta cctaaagtgc
     1261 ctaacggaac accattactg atttgcacct gatgttcgcc ggtatttaac gtcgcattca
     1321 gacccgcaga aaccagaatg agatttttca gttcgcgatg ataatagcca actaatagcg
     1381 gaaactgccc cggcagattg gcctgacgga gtaaatggtt tacctgcttc aataacgcgc
     1441 ccaactctgg caaccgttga ttttggtgtg caagctgttc ctgtaataat ccgttaaaca
     1501 atgcgcgtaa taacaaggca gcaagtacgc cattatgtcc agctcgggtg acatcaaggc
     1561 aataaaatgc cagatcgttt tccgaaagtg cggcaatatc aagcaccagg ccgggtttgt
     1621 ccgcggcaac taattgacga taattaaccc ggcattggga aatcacctgc tgaactggcg
     1681 gttgtagttc ctgtaataat ttcgccgctg cagcagggtt atcaaccatt gcatcccagt
     1741 cgcgaaaaag cctttcctct tcctcaacgc gggaattaaa catgctggga tagagacagg
     1801 caaaaaccat ctcgcgcaag cgattcagat ctttaaccgg tttcagcaaa acatcttcaa
     1861 cacccagacg taacgctttg gcaatatctg ccatattttc agtggcagat atcaccagaa
     1921 ctggtgtctg gtcacctctg ttgcgtatat gttccagcag tttaagcccg ttcattcgtg
     1981 gcatcgcgat atcacatatc atcaggtctg gagtgaaacc tcccagcaac tcaagggcat
     2041 ccaccccatc agccgccagt accgttgtcg ctcccaatga ggaaaaccac gaatccagaa
     2101 gcgagcgaaa tacctgctca tcttcaacaa tgagaatctg ttttccgacc aatggctgcg
     2161 tcatgttctc tcccctgact ggctttactc aatagtggca cgctattgcc acctacgcct
     2221 gtcagaatta acttaagtgt agataaaaat tctggtcaaa tgttggtgcg taccaacggc
     2281 aaaagttcgt ccattttctt ttccactgcc agctgtccgg ccgcaatggc agcgtgcgcg
     2341 cgatggaaat caagcgtaga tatttgcggg caaacaggtt gaatcagaat atcgggcgga
     2401 tcacctgcca tgcggttcct tttaagtcgg ttctccagca cctggataga agtggtcata
     2461 atctctgttg ccgttggcgc tgtcaccgcc cgacgtgtcg ttatgctgcc taacctttct
     2521 ttcagacgcg catgccacgg cagagaatca ccattctcgc tattttcttc actgacatta
     2581 aaggagagca aatcttgttg catcaaatga gcatcgtgct gcaggtcgac cgctatcaca
     2641 atatcagccc ccaatgcacg cgtgagggaa ataggaattg ggttaactac agcgccatca
     2701 accagccagt agccgttatg cgcaacgggt gccattagtc ctggaatact gcatgatgca
     2761 cgaatggcaa gatggagatc gccttcagta aaccataatt cacgcccagt acttaaattg
     2821 gtggcaacag ccgcgaagcg acgggaacaa ttttcgatct ctgtttccgg cattatttcg
     2881 cgatattgat tgaagacacg ctcgccgcgc agtaacccac cgcgctgcca ggagagatcc
     2941 atcaggcgta aaacatccca ataactgaaa gaggtcaccc aatcttccag cgcagataat
     3001 cgatcgcatg cataggcagc gcccaccagc gaaccaattg aacatcctgc aacgatatca
     3061 atttcaatac ccactttttt tagcgcatta attacgccaa tatgcgacca acctcttgcc
     3121 gcgccagatc ccagcgccag ccctattttt atctttctca ttattcccgc aagattcccc
     3181 tggaatgcaa tcgtagccac atcgctactg ctcggttaac atagtgctac tcaggcggtt
     3241 aatacgccgt tattgtttcc agggagatta tttgtgtctc agctttgtcc ctgtggtagt
     3301 gctgtcgagt atagcctatg ttgccaccct tatgtgtctg gtgaaaaggt tgcacctgat
     3361 ccagaacatc tcatgcgttc gcgttactgc gcttttgtga tgcaagacgc agattattta
     3421 ataaagacct ggcatccatc ttgtggagca gcggcgttac gtgccgaatt gattgccgga
     3481 tttgcgcata ctgaatggtt gggcttgacc gtatttgaac attgctggca ggacggcggc
     3541 aatatcggat ttgtcagttt tgttgctcaa tttaccgagg gtggcaaaac aggtgcaata
     3601 attgaacgtt cgcgattttt gaaagaaaac ggtcaatggt actatattga cggtacacgt
     3661 ccgcagtttg gccgcaacga tccctgccct tgtggttcag gtaaaaaatt taaaaagtgc
     3721 tgcggccaat aatggttgac ggtacggttt agcaaacact ctcaacaagg tttttccagc
     3781 aatgcattca ctccaacgta aagttctgcg tactatttgt ccggaccaaa aaggtctgat
     3841 cgcacgtatt accaatattt gctacaagca cgagttaaat atcgtgcaga acaatgaatt
     3901 tgtagatcat cgtaccgggc gcttttttat gcgcacggaa ctggaaggga tttttaatga
     3961 ttccaccctg ctggcggatc tcgatagcgc attgccagaa ggctccgtgc gtgagctgaa
     4021 tcctgccggt cgtcgccgga tagtaattct ggtcactaaa gaagcgcatt gccttggcga
     4081 tttgttgatg aaagccaatt acggcggcct ggatgtcgaa atcgcggcag ttattggtaa
     4141 ccacgatact ttacgttctc tggttgagcg gtttgatatt ccgtttgagc tggtaagcca
     4201 tgaagggtta agccgcaacg agcacgatca aaagatggcg gatgccattg atgcttatca
     4261 acctgactat gtggtgctgg cgaagtatat gcgggtatta acgccggaat ttgtggcacg
     4321 cttcccgaat aagatcatca atattcacca ttcattccta ccagcgttta tcggcgcacg
     4381 tccttatcac caggcctatg agcgtggcgt gaagattatt ggcgcaaccg ctcactatgt
     4441 gaatgacaat ctggacgaag gcccaatcat catgcaggac gttattcatg tcgatcatac
     4501 ctacacagct gaagatatga tgcgcgcagg tcgtgacgtc gagaaaaacg tcttaagtcg
     4561 tgcgctatac aaagtactgg cgcagcgcgt ctttgtttac ggtaatcgga cgattattct
     4621 ttaatcgcca gcaaaaataa ctggttagct ttaatccgtt acggatgaaa attacgcaac
     4681 cagttcattt ttctcaacgt aacactttac agcggcgcgt catttgatat gatgcgcccc
     4741 gcttcccgat aagggagcag gccagtaaaa agcattaccc cgtggtgggg ttcccgagcg
     4801 gccaaaggga gcagactgta aatctgccgt catcgacttc gaaggttcga atccttcccc
     4861 caccaccatc gctttcaaaa gcccctgaat tctcaaacga atccgcaatt aaatattctg
     4921 cccatgcggg gaaggatgag aagcttcgac caaggtttga cttgagcgcc agcgagagag
     4981 cgttgccgca ggcaacgacc cgaagggcga agcgcgcagc gctgagtaat ccttccccca
     5041 ccaccatcac ttcctgtaaa tactcaaaac ctgttactcc gagctaattc tgcccgatgg
     5101 caccgcgttt accggaccta aaatatcctc ccctgaagtt cggataagcg aagcgcatcc
     5161 gacaactcac cccgcaccgt gcccataaaa aaaccccgcc gaagcagggt ctatgtgaac
     5221 taaaattcgc ttagtgacga gcgcgcacca gctgatactt acgcgtcaga tactccaccg
     5281 ggacgctcca gatgtgtacc agacgcgaga acgggaacag caggaataac gtcatcccca
     5341 acaccaggtg cagacggaag ataaacgcca cgccatcaag atgttgagaa gcgccaccgt
     5401 ggaaggtcac taccgactgc gcccagccaa ccagtttcat catctcgcta ccgtccatat
     5461 gctgggcgga gaacggaatg gtcagcaggc ccagcgcgca ctggataacg agcagcgaca
     5521 ggatcaggat atccgctccg gtagtcgttg cacgcacgcg tgggctgaac agacgacgtt
     5581 tcaacaacag cacgccgcca atcagacaca gtacgccgct ggcaccacca gcaaacatcg
     5641 ccattttctg ttttacttca atcggcagcc aggcttcata catccagtgc ggcgtcagca
     5701 taccgaagaa gtgaccgaca aaaatcccca gaataccgat atggaacagg ttcgacgcga
     5761 ggttcatccc tttgcgatcc agcatctggc tggacgccgc acgccatgtg tactgcccgt
     5821 agtcataacg cagccagcta ccaatcagga agaccgcccc ggctatgtac gggtagatat
     5881 caaagaagaa catattcagg aattgcatta gtgctgtcct ccggtggtga tattcagata
     5941 ttgcggcgcg acggcaccgg caaagcgacg ctgatgagca gtgattgctg aatcaccgca
     6001 gcctttttca gcaaagaatt taacctgctc ttcttcccag actgcatcca gcgcctgcgg
     6061 cgtatcgtcg cgcgcttcat cggcaatttt ttccgccact ttgtcgctgt cgattgcggt
     6121 attcgccagt ttcagcagca gatcaaacag cacggcataa cggctttcac gctgttgcag
     6181 acgcgcgctc agcaatgcca gaatcggcgc aatatctttc aaaccctcca cggcttcgct
     6241 ttgcggcagc tgcgccaggt actccagata cagcggcaga tggtcgggca attcgcggct
     6301 gtttaactgc aagccgtgct gctcgtactg cgccagcaga tccaccatcg cctggccgcg
     6361 gtcacgggat tcaccgtgaa catgttcaaa tagcaacagt gacgtggcgc ggccacggtc
     6421 gaacagttcg ctgtactgcg cctgggcatc gagcggatcc atcgtcgtta aatcgcgcag
     6481 gaaaatgccc agcgcatggg catcctcttt tggcagattt ttcgacgcgg caatcgcctc
     6541 aaacatctct tgttgatgct gccataaggc agcatccgga tattcaagga gacgcgatac
     6601 aatcacgagt tcgatcatgg gtgcggctcc gttttgctgg ttacgtcgat ggcatcgata
     6661 cggcggctgt tgaacagatt gaatttggta tctgaaccgt ggcagccatc accaaaggta
     6721 aagccgcagc catttttctc cgggaaggct tcccgcgcca gttcacgatg actgctcggc
     6781 accacaaagc gatcttcgta gttggcaata gccagataac gatacatctc ctgtgcctgg
     6841 gcttcggtca gaccgacctc ttccagcgca cgggtatcga ctttaccgtc aacggtttca
     6901 gcacgtttgt aatgacgcat cgccagcata cgtttcagcg cgcgcagtac cggtttggta
     6961 tcaccggcgg tcagcagatt cgccagatac tgtaccggaa tacgcaggct ttcgacgtct
     7021 ggcagaatgc cgttgctgcc cagttcaccc gcgtctgccg cagactgaat cggcgacaga
     7081 ggcggcacgt accagaccat cggcagtgtg cgatattccg gatgcagcgg cagcgccagt
     7141 ttccattcca tcgccatttt ataaactggc gactgctgcg cagcctcaat aacgctcagc
     7201 ggaataccgt ctttaatcgc ctgctcgatg actttcggat cgttcggatc gaggaacaca
     7261 tccagctgac gctggtagag atctttctcg ttctcggtac ttgccgcacg ttcaatcgcg
     7321 tcggcatcgt acagcagcac gccaagataa cggatacgac cgacacaggt ttctgaacat
     7381 attgtcggct ggccggcttc aatacgcgga tagcagaaga tgcacttctc agacttaccg
     7441 ctcttccagt tgaagtagat ttttttgtac gggcatccgg tgatgcacat acgccagcca
     7501 cggcatttat cctggtcgat caggacgatg ccatcttctt cacgcttgta aatcgcaccg
     7561 ctcgggcagg tcgccacaca tgccgggttc agacagtgtt cgcacaggcg cggcaaatac
     7621 atcatgaagg tgttttcgaa ctggctatac atcgccttct ggatgttgtc gaagttcttg
     7681 tctttcgcca gcttgtcaaa ctcaccgccc aggtcatctt cccagttcgg ccctttttcg
     7741 attttcgcca tccgttcgcc ggtaatcagc gaacgcggac gggcaaccgg ctgtgatttg
     7801 ctgccttccg gcgcggtatg caggttctga taatcaaaat cgaacggctc gtaataatcg
     7861 tcgatccccg gcagatgcgg gttagcgaag attttaccca gcagcatggc acggttaccc
     7921 atgcgcggct gcagtttgcc gttgatttta cggatccagc cgcctttgta tttttcctgg
     7981 ttttcccagt cagtcgggaa gccctggccc ggcttggttt ccacgttgtt gaaccacgcg
     8041 tattccacgc cttcacggct ggtccagacg tttttacagg tcactgaaca ggtgtggcag
     8101 ccgatgcact tatcgagatt cagcaccatg ccgacttgtg aacgaatttt cattttacgc
     8161 tctcctgtac ctggtcattg ccttcgccat ctaaccagtc aatgttcttc atcttacgca
     8221 ctacaacaaa ctcatcgcgg ttagaaccta cggtgccgta gtagttaaag ccatatgcca
     8281 gatgggcata gccgccgatc atatgcgtcg gtttcggcgt gatacgggtg accgagttat
     8341 ggataccacc acgctgttgg gtaatttccg aaccaggcag gttaacgata cgttcctgcg
     8401 cgtggtacat catggtcatc cctgccggaa cacgctggct gacaaccgca cgggcagtca
     8461 gtgcaccgtt gctgttgaag acttcaatcc agtcgttatc ggcgataccc agctctttgg
     8521 catcagcttc actcagccag accaccggac caccgcgacc taaagtcagc atcagcaggt
     8581 tgtcgctgta ggtggagtgg ataccccact tctggtgtgg cgtcaggaag ttaagcgctt
     8641 tttccgggtt gccattggat ttctggccca tcacttcttt caccgaacgg gtgtcgatcg
     8701 gcggacgata aaccagcagg ctttcaccga aatcacgcat ccattggtga tcctgataca
     8761 gctgctgacg gccagagagc gtacgccacg ggatcagctc gtgaacgttg gtgtaaccgg
     8821 cgttgtaaga aacgtgttca tcttcaaggc cagaccatgt cgggctggag atgattttgc
     8881 gcggctgtgc ctgaatatcg cggaagcgaa tcttctcgtc ttctttattc agcgccagat
     8941 gcgtatggtc acggccggta aactcgctca gggcagccca ggcttttaca gctacctgac
     9001 cattggtttc cggtgccagc gtcaggatca tctctgccgc atcaattgcg gtattgagca
     9061 tcggctggcc ttttgccgga ccttccgctt tggtgtagtt gagcttacgc agcagatcca
     9121 tctcgctctg ggtgttccag gcaatccctt taccgccgtt accgattttc tccatcagcg
     9181 ggccgataga ggtaaagcgt tcgtaagttg ctggataatc gcgctctacg accataatgt
     9241 gcggtgcagt tttacctggg atcaggtcgc attcgccttt tttccagtct ttcacatcca
     9301 gcggttgcgc cagttcagcg gcagagtcgt gctggatagg cagcgtgacg atgtcagttt
     9361 ctttacccag atgaccaacg cacacttcgg agaatttctt cgcgatggct ttgtagattt
     9421 cccagtcgct tttcgcttcc caggccggat cgaccgccgc agacagcggg tgaataaacg
     9481 gatgcatatc cgaagtattc atgtcgtctt tttcgtacca ggtcgccgtc ggcaaaatga
     9541 tgtcggaata gagacaggtg ctcgacagac ggaagtccag cgtcaccacc agatccagct
     9601 tgccttccag accgttgtcc tgccagtcca cttcttccgg cttcacgccg ccctgctgcc
     9661 ccaggtcttt gccctggata ccgtgctccg tccccagcag gtacttgagc ataaactcat
     9721 gacctttacc ggaagaaccg agcaggttag aacgccagat gaacagatta cgcgggtggt
     9781 ttttaccgtt ttccggctgt tccgcagcga aacggataga accctctttc agggatttca
     9841 ccgtatagtc caccggattc atcccggctt tttctgcttc gcgagcgata gtcagcgggt
     9901 tagtgcctaa ctgcggcgca gacggcagcc agcccatgcg ttctgcacgg acgttaaagt
     9961 cgatcaagtg tccggtatag cgagatttat ccgccatcgg cgacagcagt tcttccgcag
    10021 tcacggtttc ataacgccac tggctggagt ggttatagaa ataagaggta ctgttcatgt
    10081 gacgcgccgg acgctgccag tcgagggcaa acgccagcgg ctgccagccg gtttgcggac
    10141 gcagtttttc ctgacctaca tagtgcgccc agccaccgcc gctctgaccg acacagccgc
    10201 agaaaatcag catgttgatc agaccacgat agttcatgtc caggtgatac cagtggttca
    10261 gcccagcgcc gacgataatc atcgaacgac cgtgcgtttt atcagcgtta tcggcaaatt
    10321 cacgggcgat gcgaataatc tggctgcgag aaacgccggt aatctgctct gcccaggcag
    10381 gggtgtaagc tttcacatcg tcatagctgg ttgcacagtt aacgtcgttc aggccacgtt
    10441 ccagaccgta gtttgccagc gtcagatcat aaacggtggt caccagggcg gtgctgccat
    10501 cagccagttg caggcgtttc accggcagct tatgcagcag cacgttttcc agttccactt
    10561 tgttgaagtg ttccgtgcca tcgccaccaa agtacgggaa gcccacctca gcgatctcat
    10621 cctggctacc cagcaggctc agttgcagct cggtttcttc gccagttttg ccgtcgcgct
    10681 gctcaagatt ccatttgccc ttctcgcccc agcggaagcc aatagaaccg ttcggcgcaa
    10741 ccatttcacc attggtatta aaggcgacag ttttccattc cggattgttt tcctggccca
    10801 gtgcatccac cagatcagca gcgcgcagca tacgacccgc agcgtagtaa ccgtcgcgct
    10861 cttccagcat caccagcatc ggcatgtcgg tgtagcggcg cacatagtcg gtgaaatact
    10921 ggcttgggtt gtcgaggtgg aattcacgca gcattacgtg acccattgcc agcgccattg
    10981 ccgcatcggt gccctgtttc ggtgccagcc acagatcgca cagtttggcg atttcagcgt
    11041 agtctggtgt gacggcaaca gttttggtcc ctttgtaacg cacttcagta aagaagtgag
    11101 catccggggt acgcgtctgc ggcacgtttg aaccccaggc gatgatgtag ctggagttgt
    11161 accagtcagc agattccggt acgtcagttt gctcacccca ggtttgcgga gacgcaggag
    11221 gcaggtcgca gtaccagtcg tagaagctta agcaagtacc gccaatcagc gagagatagc
    11281 gcgcacccga tgcgtaagaa accatcgaca ttgccggaat tggcgagaag ccagcgacac
    11341 ggtccgggcc gtaatttttg atggtgtaaa cgttagatgc ggcgatcagt tcgttcactt
    11401 cctgccagga agaacgaaca aaaccaccgc gtccacgcgc ctgcttaaag cttttcgctt
    11461 tatcggcgtc ttcaatgata gaagcccatg cctcaaccgg atcgctatgc agcgccttcg
    11521 cttcacgcca cattttcatc aggcgtttgc gcatcatcgg gtatttcagg cggttagcgc
    11581 tgtatagata ccaggagtag ctggcaccgc gcgggcagcc gcgaggttca tggtttggca
    11641 ggtccgggcg agtacgcgga tagtcagtct gctgggtttc ccaggtgacc agaccgtttt
    11701 tgacgtagat tttccagctg caggagccgg tgcagtttac cccgtgggta gagcggacga
    11761 ttttgtcatg ctgccaacgc tggcgatatc catcctccca gtcacggttg gtattgagaa
    11821 gctggccatg cccatcggca aaggtttcac ccttctgttt gaagtagcga aaccggtcca
    11881 ggaatttact catcggtttt ctcctgtggg agcctgtcgg ccctctgata aatcgacatt
    11941 gctaatgttt aagggcaagg taacgctctg aaacagcgtg ggaattgata acgatcaaga
    12001 gtgatgggga tgaaaaataa agtaaattcc tggaataccc ctttaggata attaacattt
    12061 atagcataac agactgtaaa ttaagacgtt ttacacctgt agttatttaa accattaaga
    12121 tttttatgca gatgggtatt aaggagtatt ccccatgggt aacatattaa tgactcttaa
    12181 tgaaatccag cgaaataagc catacaacat tagtatgttg taactaaatg acgcataaaa
    12241 aaagcgcgac atcatgccgc gcaaaggata atcaaatatt acgatttatt ttttagaatg
    12301 ccgaccatat accgcccagg taatcaccac gcaggcgata tagaaaatca aaaatacttt
    12361 cattgcgccg actggcgaac ccgttaatgc cagcgagcta ccaaacgctt tcgggataaa
    12421 gaagccacca atcgcgccaa tcgcagagat gaaacccagc gccgccgccg tgtcggttgc
    12481 cgcttcacgc atcgcacgtt cgtcagaacc cccttctgct ttcacacgat ccattgtcag
    12541 tttacggaag atcacggaaa tcatctggaa agtggaacca ctacccagcc cagcggtcag
    12601 gaacagcgcc aggaagactg cgaagaacgc catgaagctt ccgccctgtc cgtcagtcgg
    12661 taaggtcagg aacagcaggc cgctgaaaat cgccatcagg ataaagttca ccagagtgac
    12721 acgagttccg cccagacggt cagataatgc accacctgca gaacgcgcca gcgcaccaat
    12781 aaacggcccg aagaaagcgt attgcagaat ctgaatatcc gggaactgcg tttttgacag
    12841 catcgcaaag cccgcggaga agccgatgaa ggagccgaag gttgccagat acagcaggct
    12901 cataatccac agatgacccc gtttgagtac cggcaattgc tccttgatgg aggctttcga
    12961 ggtagcaaga tcgttcatgc caaaccacgc cgcaatggtg aagatggcaa ggaacggcac
    13021 ccatatccag gacgcattcg ccagatacag ctcagtccca tccggctgtt tgacgccctg
    13081 gctaccaaat actgcgaaaa tcgacagtga taccaccagc ggagcaacca actgcatgac
    13141 gctgacgccc atgttgccca gaccaccatt cagacccagc gcgccaccct gcttctgttt
    13201 cggaaagaag aagctgatgt ttgccatact ggatgcgaag ttcgcgccag caaagccgca
    13261 cagcagagaa atgatgatga agacgctata aggcgtggag gtatcctgca cggcaaaacc
    13321 cagccagacg caaggaataa tcagaatacc ggtgctgaac gccgtccagc gacgaccacc
    13381 aaagatagga accataaagg agtatggaac acgtaataac gcgccagaaa ccgaaggcag
    13441 cgcagtcaac ataaatagct gatcggtcgt aaaattaaaa ccgactttcg gtaagttcac
    13501 cgcaacagcg ctgaacaaca tccatacgca aaacgccagc aacagacagg gaacggaaat
    13561 ccacaggttg cggctggcaa tacgttgacc gcgttgttgc cagaacgcag gatcttccgg
    13621 tcgccaatct gtaatgacag ctccagtagc cctttcgggg gcggatgagt gactcataga
    13681 cacctctgat actcgtttcg atgtctgcca ccttagtgtc tgtagctaaa ggcaatttga
    13741 tgtaaatcaa acgataagat aactttatct atcattgata tttatcatta tccatagtga
    13801 gtacagtgat ttcataaaaa ttatgagatt tttcacggtg ctgtaaaatc cctaccctta
    13861 ccgatgtaaa gcgactaacc acacggcaaa taaggagtaa ctctttccgg gtatgggtat
    13921 acttcggcca atagccgaga atactgccat tccagaatgt atcatcacat tcattaaggt
    13981 tattgctcat ttaaagcctg aaggaagagg tttacatgct taaacgttgt ctctctccgc
    14041 tcaccctggt taatcaggtt gcgcttattg tgttgctttc tactgctatt ggactggcag
    14101 ggatggcggt ttctggctgg ctggtgcaag gcgttcaggg cagcgcccat gcgatcaaca
    14161 aagcgggatc gctgcgcatg caaagttacc gtctgttggc agcagtgcca ttaagcgaga
    14221 aagacaagcc cttaattaaa gagatggaac aaacagcatt tagcgccgag ttgactcgag
    14281 cagcagaacg agacgggcaa ctggcgcaat tacagggttt acaagattac tggcgtaatg
    14341 aactgatccc tgcactgata cgtgcacaaa accgcgaaac ggtgtcagcg gatgtcagcc
    14401 agtttgttgc cgggcttgat cagctggtat ctggttttga tcgcaccacg gaaatgcgca
    14461 ttgagacagt ggtgctggtc catcgggtaa tggcggtatt tatggcactt ttactggtat
    14521 tcactattat ctggttgcgg gcgagattgc tacaaccgtg gcggcaactg ctggcaatgg
    14581 cgagtgccgt cagccatcgc gattttaccc aacgcgcaaa caccagcggg cgcaacgaaa
    14641 tggcgatgct tggaactgcg ttgaacaata tgtctgcaga actggccgaa agttatgccg
    14701 tacttgagca gcgggttcag gagaaaaccg ccgggctgga gcataaaaat cagatcctct
    14761 cttttttatg gcaggctaac cgccgtttgc attcccgcgc cccgctgtgt gaacgcctgt
    14821 cacctgtact caacggatta cagaatttaa ccctgctacg tgatatcgaa ctgcgggtgt
    14881 atgacactga tgatgaagag aatcatcagg agtttacctg ccagccagat atgacttgtg
    14941 atgataaagg ctgccagctc tgcccgcgcg gcgtattacc cgttggcgat cgcggcacaa
    15001 ccctgaagtg gcggctggct gacactcata cgcagtacgg tattttgctg gcgaccctgc
    15061 cgcaggggcg tcatcttagc catgatcaac aacaactggt ggataccctg gttgaacaac
    15121 tcaccgccac gctggcgctg gatcgccatc aggaacgtca gcaacagttg atcgtgatgg
    15181 aagagcgtgc caccattgcg cgcgaactgc atgattctat tgcccaatct ctctcttgca
    15241 tgaagatgca ggtgagttgc ttacagatgc agggcgatgc gctgccagaa agcagccgcg
    15301 aactgttaag tcagatccgt aacgaactga atgcatcctg ggtgcagttg cgtgaattgc
    15361 tcaccacatt ccgtttgcag ctcaccgagc ctggattacg tccggcgctg gaggcgagtt
    15421 gcgaagagta cagcgccaaa tttggcttcc cggtgaagct ggattatcaa ttgccgcctc
    15481 gtctggtgcc ttcgcatcag gcaatccact tgttgcaaat tgcccgtgag gcattaagta
    15541 acgccctcaa acattcgcaa gcgagtgagg tcgtggtgac ggtggcgcaa aacgataatc
    15601 aggtcaaact gaccgtccag gataacggct gcggcgtgcc tgaaaatgcc atccgcagca
    15661 atcactacgg catgataata atgcgcgacc gtgcgcaaag tttacgaggc gattgccgcg
    15721 tccgccgtcg tgaatcaggt ggcaccgaag tggtggtcac ctttattccc gaaaaaactt
    15781 tcacagacgt ccaaggagat acccatgagt aatcaggaac cggctactat cctgctgatc
    15841 gacgatcacc cgatgttgcg aactggcgta aaacagctta tcagtatggc accagatatc
    15901 accgtggttg gcgaagcgag taatggcgaa cagggtattg aactggcgga gtctcttgat
    15961 cccgatctga tcctgttaga tctcaatatg cccggcatga acggtctgga aacgctggat
    16021 aaactgcgcg aaaagtccct ctccgggcgc attgtggtat tcagcgtctc taaccatgaa
    16081 gaagatgtgg tcaccgcact gaaacgcggc gcggatggct atctgttaaa agatatggaa
    16141 ccggaagatc tgctgaaagc gttacatcag gcagctgctg gcgaaatggt attaagcgaa
    16201 gcattaacgc ctgttctggc cgccagcttg cgcgctaacc gtgccactac tgagcgcgat
    16261 gttaaccagt taaccccacg cgagcgcgat attctcaagc taattgccca gggtttgccg
    16321 aacaagatga ttgcccgccg cctggacatc accgaaagca cagtaaaagt tcacgtcaag
    16381 cacatgctga agaaaatgaa gctaaagtct cgcgtggaag ctgcggtatg ggtgcatcag
    16441 gagcgcattt tctgattacg gttcccagcg cagttcgtcg tttgacaatg cgtcgaacgg
    16501 ttcgacaagc gttagcgtga tctcattgga ggagacacgc tgcccctggt tatcttccac
    16561 caccactgac aatcgccagt gattgcttgc gccttccccg ttttgccagt caggcatgat
    16621 cagcgtccag ccctcctcac tgttggcttg tgcgcccggc gtcaaactta atatctgcgt
    16681 atcgccctgc caaatcagtt gccgaatacc gtaacgactg cggatttgta atttcagcgg
    16741 cactgtttcg ccaggtttta gatcccacgg cggtgtcgcc agaaacaccg tcaacgtttt
    16801 tcgctgtcgg tactcaagag tcggaagatt atttcgctgc ggattgtcat agcgactacc
    16861 acgtaacgac tgactttcgg caacctcgcc cgcagaaagt tgttttttga gcggtacacc
    16921 aaagcggtag ttaagattca gcccgaggtt attttgattc tcgccacttt caccctgttt
    16981 atgctgggcc gtcacagtga ctaagggcac aggggtgtaa tttaatccca gactcaacgc
    17041 gacgggattg tgataacccg taccagagtt aaacaaatcg acacgatcac caaaatactg
    17101 ttctacgctg acactggtat tgaggtgttg atagaacggc atgcgcatcc gggctgtcag
    17161 gtcgtacccg cgcgccatcc gttgttcctg cgtggctgtc tgttcatgcc atgcagcaaa
    17221 cggctgataa aagtttgccg atagtcgcaa atattcgccc cacgcttcag cgccaaagcc
    17281 cgctcgctga agattttcgt ccagcaagtt gtcataaaaa gtgttataac ccaccagcca
    17341 gctgccgcgc gcccagcgtt gcccaacgcc cacattgctc accagcccat catcctgctg
    17401 agtaagacca agctggctcc aggtgagata acgatcatta tcttgtaacg gcacaaacca
    17461 gcttccacga ctgccggtga aatgtccttc gttatccact ttgacgtcaa cactggcatt
    17521 tccccacggt gatagccagg actctacgtg ctgattaacc tgttggctaa gcgcgtcgcg
    17581 gacctttccc aatgcgaaag cttttgcctg ctcgccagta tcaagcccgt tatcattcat
    17641 actggtttcg ccgaaatctt tcacaatttc agcaaagtgt ttttccccat catgattttt
    17701 aggtgccatg cctaaatccg gcagaccatc attgttatta tcaaagggat ttgccgcttt
    17761 ttgctcgaag gtagattgtg cgttagctgt accgcctgcc gccagcaaga gtaaataaaa
    17821 cggaataatg cgaggaatga aacggctcaa aacgtcggta acttccggta aaaatgaaaa
    17881 accatagcct taaagaataa cgattactgg caccttttgt cgtattttct cgcgctttca
    17941 ggaaaatcct taaaaatgtg accgattggt cagacgctgc agaagcagta agatagaaga
    18001 tgagtgtcta attccctgaa tacaggagta aataatgcaa aaaatcgtga tcgttgccaa
    18061 tggcgcacct tacgggagcg aatccttgtt taacagcttg cggctggcca ttgcgttacg
    18121 agagcaggag aacaatctgg atctgcgtct gttcctgatg tctgatgcgg taacagccgg
    18181 gttgcgcggg caaaaaccag gggaaggcta caacattcag caaatgctgg agatccttac
    18241 cgctcagaat gtaccggtga aattgtgcaa aacctgtacc gacgggcgcg ggattagtac
    18301 acttcctctg attgatgggg tggaaatcgg tactcttgtt gaactggcgc aatggacgct
    18361 gtcagccgat aaagtgctca cattttaatg tttgaggggg cgtcggacgc cccactatga
    18421 gggaagttta ctcaggcgaa tcccggccgt aacacaccat ccggataatt ctccgccagc
    18481 agttttttta ccgataccag caaatcattc agcccatcgt cctgcattcc cagtttgata
    18541 agctcctgtt ccagtgaaaa caggtattgt gcattggttc ccagcggacc gctcgccgcc
    18601 gcaatcaacg gcgctatgac ctgagcgcga gtgtcagatt catattctgg atgtcgcggg
    18661 tccataataa acacaatggc gtttactgtg cgtccatcat caagatcaag ctgacaccag
    18721 gttggcagat aacagccggt aatcatctcg cgcttccaca gcagggttag ttcctgctcc
    18781 agcgtctctt ctggcagtcg ataggcgacg cctgtggtgc gtccgccctc tttcagtgca
    18841 agcattcgtc ccggctggtg cgcagtcccg cgcccggcgg tcaggcgcag gcaaaacgcg
    18901 cgatgccatc caaccagtgt accggtgcac gactcggtaa actccagtgc cggattccac
    18961 atcaacgatc cataaccgaa gatccacacc ggtccctcat caggtcgaca agccagcgtc
    19021 gccgccagcg aagccgcccg ttgttctgct gaccataaga gtgattcttc aatggcacca
    19081 aatgccgttt tacaatcggc attcatcaag aaatcacgcg ttatcacctt acacctccgc
    19141 cactgcatac ttccctgcga caactttttg gtgtcttccg gacattttta ctgctcattt
    19201 tgaaaccact aacgggacaa tatgcaatcc acggaccact tgcaaggcaa caccgcacga
    19261 atagctttag ttttacgatt ttttatgcca tttatcatca tcccctttga catattcatg
    19321 cttcacagca gcccaggcca ctttatgcgc tgtttcttcg cgactggcgt catcacgccg
    19381 atcttcttta tctttatatt gatcccatgc gctgttgaac gcttctttat agatatcctg
    19441 ggcatgagac ggaagcacgt gctttacgct ttccggcaga tcgcttttcg ttttatacgg
    19501 catcgttgac tccttttaca aagttgaaca ttaagtgtgg tcagcaatat ctactcttgc
    19561 caaaacataa gaattttcat gtcagactct tcttaagaag tgctaaaaca aacattgtct
    19621 aaacgtatta cttattttta tgcccaaatg agtaaaaaat cgacagcggg gtaaaaattc
    19681 gtaaaatgtt accgatatcc agacaatttc tgttattttt cctacctatt ttttacattt
    19741 tcgataaatt catctgcaaa gggataacca taatgtcaaa tgctcaagag gcggtaaaaa
    19801 cccgccacaa ggagacttcg cttattttcc cggttctggc gctggtagtg ctgttcctgt
    19861 ggggaagcag ccagacacta ccagtggtca ttgccatcaa tcttcttgcg cttattggta
    19921 ttttaagtag cgcctttagt gttgtccgtc atgcggacgt attagcccat cgcctgggag
    19981 aaccttacgg ttcgcttatt cttagccttt cagtggttat tcttgaagtc agtttgattt
    20041 cagctttaat ggcaaccggc gacgccgcgc caacgctaat gcgtgatacg ctctattcaa
    20101 tcattatgat tgttacaggt gggctggttg gcttttcatt attgttgggc ggacgtaagt
    20161 ttgccaccca atatatgaat ctgtttggta tcaagcagta tttaattgcg ctgttccccc
    20221 tggcgataat cgtactggta tttccaatgg ctctgcctgc ggcgaatttt tcaacaggtc
    20281 aggcgttact ggtagcatta atttctgcgg caatgtatgg cgtatttttg ctgatccaga
    20341 ccaaaacgca tcaaagtttg tttgtctacg agcacgaaga tgacagtgat gatgacgacc
    20401 cgcaccacgg taaaccgtct gcccatagca gcctgtggca tgccatctgg ttgattgtcc
    20461 atctgattgc cgttattgca gtcaccaaaa tgaacgccag cccgctggag acattgctcg
    20521 acagcatgaa tgcgcctgtc gcctttactg gcttcctggt ggctctgttg attctgtcgc
    20581 cggaaggttt aggtgcgtta aaagcagtgt tgaataacca ggtccagcgc gcgatgaatc
    20641 tgttctttgg ttcagtgttg gcaaccattt cgcttaccgt acctgtcgtc acgctaattg
    20701 cctttatgac gggtaacgaa ttgcagtttg cacttggtgc gccagaaatg gtggtgatgg
    20761 tggcctcttt agtactgtgc catatctcct tctccaccgg acgaactaac gtgctcaatg
    20821 gcgcagcgca tctggcactg tttgccgcct atttgatgac gatatttgcc tgacagttat
    20881 atctcctccg atgacggaaa tcgggggaga taatataaat tcctgcttat tgtatttctt
    20941 catactacct ggacaggtcg ttaatcagaa ttgcgagcct gtaccacaac ctgcacgaaa
    21001 ccactgcaac agctgttgaa aagccggaat gtactcttca atgcaaacat ttcagggatg
    21061 aagtgaagat gaattgcgac gtagtatcca caccaagccg gcgttatccg gtgaggcgca
    21121 atgttgcggg ggctttatcc ctggtggcat tggttgctgg aaagagaaaa cccccgcacg
    21181 ttgagaggta aaacctgaca acagaacggg ggctaatctt gaaccatcaa caattcaaga
    21241 atagccgcaa aatgttgtca ttacaacaag gcggctatat gacgctcgcg cagtttgcca
    21301 tgattttctg gcacgacctg gcagcaccga tcctggcggg aattattacc gcagcgattg
    21361 tcggcttgtg gcgtaaccgg aagtaaggtg aaaacgggcg tcaggctgcc gtgatggcaa
    21421 tatgcgcccg aaaccggccg caggggtaac agcctgcggc cttttttgta tccggcaccc
    21481 ataaaaaaag tcttaacgca gaacgctaag actttatttt tcaaacaaaa agattacttg
    21541 ctggtatcca gttcttcgaa acctttcacc agatcatcaa tcgctttcat ttgcttgagg
    21601 aacggttcca gtttagccag cggcagcgcg gatggaccat cacatttcgc atgttccgga
    21661 tccggatgcg cttcaataaa cagccccgcc agacctaccg ccataccggc tcttgccagc
    21721 tcagccacct gagcacggcg accgccggaa gctgcgccaa atggatcgcg acattgcagt
    21781 gcgtgggtaa cgtcgaaaat caccggcgaa ttaccagaca ctttcttcat gatgctgaag
    21841 cccagcatat caacaaccag gttgtcatag ccgaagttag caccgcgatc gcaaagaatc
    21901 actttttcgt tgccgccttc tttgaattta tcaacgatat tacccatctg tcccgggctg
    21961 acaaactgcg gtttcttgac gttaattacc gcaccggttt tcgccatggc ttcaaccagg
    22021 tcagtctggc gagcaagaaa cgccggcaac tgaatcacat ctacaacatc agcaacgggc
    22081 tgtgcctggc taggttcgtg aacgtcggtg ataattttca cgccaaaagt ctgcttcaac
    22141 tcctggaaga ttttcatccc ttcttccagg cccggtccac gataagagtg gatggaggag
    22201 cggttggctt tgtcaaaaga ggctttgaac acgtaaggga tacccagttt ctgggtcacg
    22261 gttacgtagt gctcgcaaat gcgcatcgcc agatcgcgag attccaacac gttcataccg
    22321 ccaaacagta cgaacggcag gtcatttgct acgttgatgt cgccaatgct aaccactttt
    22381 tgtttcatag gatcgcctta atcttgagta aaatgtcgat taattaatgc agcacaatat
    22441 gtttatgcgc gatgttattt atttgcgcac ggatcatttc gctgatcggg tcttccggac
    22501 actgttcaac gaaataactt aaatcgttca acgcaacgtg ttcgcaatcc agttgcgcat
    22561 aaatcaaccc gcgatcgcga atttcatagg gatcttccgg gttgaattgt aataaagctt
    22621 cgctggtgcg taacgccagc tccatctgat tttcttccat caacgaagct ttgagtgtat
    22681 ccagcaattt gcggattacc tcaatgttat cagcttcatc aaggtcttca taaaacagtt
    22741 ccgccgacgg gctgatattt ccctttaacc acacatccag catatgttcg cttaacgatt
    22801 caccgttaaa aggattaatc agccaaattt cgccatccgg acattcaatg cgcaatatca
    22861 gctgcgtagg gaaaatcacc ggcagcagcg gcaaatcgag acgattcgcg acccataata
    22921 aaaccgcacc taatgatacc gcactgccct gtcgattctt taacacctgg tccagccata
    22981 atgcatcgga aagacgataa acgccgcgtg aggctttaaa cccccattcg ccgtaaaaca
    23041 gcgcgattaa tttttctagc tgctcttcta atggcagaag ctggctaatt tcttccttcg
    23101 ccagactaac gagacgctcc agttcgtcgt aaacatcttg cgagggaaaa tcgcggcgga
    23161 ttgcttcgca agccaggatc atgccttcgc acaatggcgc tttattaaat tcgaaatcag
    23221 ctaacgatct catgacttac cccagtaacg gtacttttgt ggtggcgagt ttaatgatga
    23281 tgtacagcac caccaacgcc agcgggaagg caataatgcg cgcctgctga ctgtgcatac
    23341 gacgataatc gagtgcaata aaacccaaaa caatataaat gataactcca aacagctttt
    23401 cagtcagcca ctgtgcctgc ccggaaaatg gcaggatgtg cgctttagcc atcaacgcaa
    23461 tgccgcttaa caataacagc gtatcgacaa ccggcggcac aatgcgcgtc cagcgcgcaa
    23521 atgcctgtgg atgcttctga taacgtagcc agaaacgtaa ggttaatagc ccaacagaaa
    23581 gcgcgatact aataagatga acactaagca gtgtagaaaa acttgtcatt gataatagcg
    23641 gccgagcgtt acgcgctcgt tatcaccata gtcccgacag gtttcgacgt catgatatcc
    23701 cgcgtggata aatgcctgtc gcaccgcttc gccctgctgc cagccatgtt ccagaagcag
    23761 aaagccgccg gataccagcg cgttacgcga ctgttcgatg atatgcacga tgtctgccat
    23821 tccactgtct gccgcaacca gcgcagtgag cggctcaaag cggacatcgc cttgttgaag
    23881 atgtgggtcc tgctcgtcaa tatacggcgg attactgaca atcatcgcaa actgctgccc
    23941 ggctagcgcg ctaaaccagt cgctttgcag aatatggatg tttttgatcg ccagattctg
    24001 ggcattacgt tgcgccagag agacagcatc tggcatacga tctacagcgg taatttcgca
    24061 gtccgggcgc tcgctagcca gcgccagcgc aatcgccccg gtacccgtcc cgagatcgag
    24121 aatacggcaa ggttgttcag gcaaccgcgc cagtgcctgc tccaccagac actccgtatc
    24181 cgggcgcgga attaaggtcg ctggcgaaac aaataacggc agcgaccaga attctcgcac
    24241 cccggttaaa tgagcaatag gttcaccatc gcgacgacgc gtcagtagcg catcaagttg
    24301 ctgacattgt tcgtcagtca gctgcgtttc accaaaggcg agaataaaag tacgcccttt
    24361 gccggtaaca tgcgccagca ggatttcagc atcacgccgc gggctttcgc tcgcctgaag
    24421 ttggcttatt gcttcacgta accagtgttg atattccatt attcctgctc ggacaacgcc
    24481 gccagttggt cggcctgatg ttcctggata atcggttcaa ttagcatatc cagcttacct
    24541 tccatcactt catccagacg gtagagcgtc aggttgatgc ggtgatcggt aacgcgcccc
    24601 tgcgggaagt tgtaagtacg gttacggtcg ctgcgatcgc cactccccag caggttacga
    24661 cgggtagacg cttcggcctg ttgccgtttt gccatttctg cagcgtggat gcgagcacca
    24721 agtacagaaa gtgctttagc tttgttttta tgttgtgaac gttcgtcctg acattcaaca
    24781 acaatccctg tcggcaagtg agtaatacga attgccgaat cggtggtgtt aacgtgttga
    24841 ccacccgccc ctgacgagcg gaaagtatct atgcgcaaat ctgccgggtt gatgtccggc
    24901 agttctgcgt caggcagttc tggcattacc gcaacggtac aagcagaagt atgaatacgc
    24961 ccctgcgatt ccgtagcagg cacacgctgc acgcgatgac cgccagattc gaatttcaga
    25021 cgaccataca caccatcacc gctaattttg gcgatgatct ctttataacc accatgttca
    25081 ccctcgctgg cgctcatgat ttctacccgc cagcggcggg cttcggcata acggctgtac
    25141 atacggaaca gatcgcctgc gaacagcgcc gcttcgtcgc cgccggttcc ggcacggact
    25201 tcgaggaagg cgttacgttc gtcatcagga tcttttggta gtaacagaac ctgtaattgc
    25261 tgttccagtt gctcgctttt ttctttagct tcgcgcagtt catcctgcgc catctcacgc
    25321 atttcaggat catcgagcat catctgtgcg gtttcgatat cttcctgaac ctgttgccag
    25381 tcggtaaaac agcgcgaaac atcacttaac tgcgcatatt cgcgtgataa tgcgcgaaaa
    25441 cgttcctggt cggcgatagt ttgcgcatca cccaacaacg cctgaacttc ttcatggcgt
    25501 tcatgcaggg cttccagttt ggcaacgata gaaggcttca taggcgtaaa tgcaccctgt
    25561 aaaaaaagaa aatgatgtac tgctactcca gcccgaggct gtcgcgcaga atattcaggc
    25621 gttcgttatc cccgtcacgg gcggcctgtt gaagtgattt cgttggcgca tggatcaggc
    25681 ggttagtcag tttccatgcc agatcctgca taatggcttg cgcgtcgccg ccctgctcaa
    25741 gggccgctaa cgctttggcg gttaactcat cgcgaacgtg ctctgcctgg ctgcgatact
    25801 cgcgaatggt ctcgctggcg ctttgtgcgc gcagccacgc cataaattcg ctggtttcct
    25861 gagcgacaat agtttccgcc tcaaccgctg cggctttacg ctgcgccagg ttgtgcgaaa
    25921 tgatgctttg cagatcatca acgctgtaaa gataagcatt cgccagtttg ccaacttccg
    25981 gctcaacatc gcgcggaacg gcaatatcca ccaacagcat tggttggttg cgacggcttt
    26041 ttaatgcccg ctccaccatg cctttcccga taatcggtaa cgggctggcg gtggaactaa
    26101 tgatgatatc ggcttcgcgc agacgctcgt cgatatcgct cagggcaatc acttccgcgc
    26161 cgacttcatc tgccagaatt tgggcacgtt cgcgagtgcg gttggcgata atcatcttct
    26221 gtactttgtg ttcacgcaga tgacgcgcca ccagctcgat ggtttcgccc gcacccacca
    26281 gcagtaccgt caccgtagag agcgattcaa agatctgccg cgccagcgta caagccgcaa
    26341 aagcgacaga cacagcactg gcaccgatat ctgtttcagt gcgaacgcgt ttcgccacag
    26401 agaaagattt ctgaaacatg cgttccagtt cgctggcctt catatgacct ttttgcgaat
    26461 cggcaaacgc ttttttcacc tgaccgagga tttgtggctc gccaaggacg agagaatcaa
    26521 gcccactggc aacgcgcatt aaatgactga ccgcgtcgtt atcctgatgc cagtagaggc
    26581 ttttgcgcag gtcttcttca ttaagattgt gataatcgca aagccagcgg attaacgcct
    26641 cttgcaggtt atcctgctct tcaacgctaa gataaagttc cgtgcgattg cacgtcgaca
    26701 gcaccacgcc gccctgcacc atcggctgcg caagcaggct gtcaagcgcc tgatcgagct
    26761 tatccggcga aaacgataca cgttctcgca gcgatacagg tgccgtttta tggttgatac
    26821 cgagtgctaa aagggtcatg tctgcgggaa ataataccaa cgttgatagg gttagtctgc
    26881 ttgcatcata caggatgcgt aagatcaata aaagagagcg cccccttttg gagtaattgc
    26941 cgaaagccgt tagattctgg caattaagac aacgtgaaca tagacgatgg ctaacggtaa
    27001 cgctagcatt aagggttata actgcaacgt atctcaagga cttgtcatca ttatgcccct
    27061 gcccgatttt cgtcttatcc gcctgctacc gctggctgct cttgtgctca ctgcctgttc
    27121 cgttaccacg cccaaaggtc ctggcaaaag cccggattcg ccacaatggc gtcagcatca
    27181 gcaagacgtg cgcaatctta atcagtatca aactcgcggc gcgttcgctt atatttctga
    27241 ccaacaaaaa gtgtacgccc gtttcttctg gcagcaaact ggccaggatc gctatcgtct
    27301 gctgctgact aacccattgg gcagcacgga actggagctg aatgctcaac cgggtaacgt
    27361 gcagttagtc gacaataaag gtcagcgtta tacttccgat gacgccgaag agatgattgg
    27421 caaattgacc ggaatgccaa ttccgctcaa cagtttgcgc cagtggattt taggtttacc
    27481 gggtgatgca accgactaca aactggacga tcaatatcgc ctgagcgaaa tcacctacag
    27541 ccagaatggc aaaaactgga aagttgttta cggtggttat gacacaaaaa ctcaacctgc
    27601 gatgccagcc aatatggaac tcaccgacgg tggtcaacgc atcaagttaa aaatggataa
    27661 ctggatagtg aaataatgcg gacacagtgg ccctctccgg caaaacttaa tctgttttta
    27721 tacattaccg gtcagcgtgc ggatggttac cacacgctgc aaacgctgtt tcagtttctt
    27781 gattacggcg acaccatcag cattgagctt cgtgacgatg gggatattcg tctgttaacg
    27841 cccgttgaag gcgtggaaca tgaagataac ctgatcgttc gcgcagcgcg gttgttgatg
    27901 aaaactgcgg caaacagcgg gcgtctttcg acgggaagcg gtgcgaatat cagcattgac
    27961 aagcgtttgc cgatgggcgg cggtctcggc ggtggttcat ccaatgccgc gacggttctg
    28021 gtggcgttaa atcatctctg gcaatgcgga ctaagcatgg atgagctggc ggaaatgggg
    28081 ctgacgctgg gcgcagatgt tcctgtcttt gtacgtggtc atgccgcgtt tgccgaaggc
    28141 gttggtgaaa tactaacgcc ggtggatccg ccagagaagt ggtacttggt ggcgcaccct
    28201 ggtgtaagta ttccgacacc ggtgattttt aaagatcctg aactcccgcg caatacgcca
    28261 aaaaggtcaa tagaaacgtt gctaaaatgt gaattcagca atgattgcga ggttatcgca
    28321 agaaaacgtt ttcgcgaggt tgatgcggtg ctttcctggc tgttagaata cgccccgtcg
    28381 cgcctgactg ggacaggggc ctgtgtcttt gctgaatttg atacagagtc tgaagcccgc
    28441 caggtgctag agcaagcccc ggaatggctc aatggctttg tggcgaaagg cgttaatctt
    28501 tccccattgc acagagccat gctttaagcc gggcaagctg agtttcggtg acaacgtcac
    28561 cttgttccag acgttgcatc gcgctcttta atacaccgcc tggaaagaat catgcctggc
    28621 ccgcacagtt ttcggcagat tctttccacc aatggacgca tgcctgaggt tcttctcgtg
    28681 cctgatatga agctttttgc tggtaacgcc accccggaac tagcacaacg tattgccaac
    28741 cgcctgtaca cttcactcgg cgacgccgct gtaggtcgct ttagcgacgg cgaagtcagt
    28801 gtacaaatta atgaaaatgt acgcggtggt gatattttca tcatccagtc cacttgtgcc
    28861 cctactaacg acaacctgat ggaattagtc gttatggttg atgccctgcg tcgtgcttcc
    28921 gcaggtcgta tcaccgctgt tatcccctac tttggctatg cgcgccagga ccgtcgcgtc
    28981 cgttccgctc gtgtaccaat cactgcgaaa gtggttgcag acttcctctc cagcgtcggt
    29041 gttgaccgtg tgctgacagt ggatctgcac gctgaacaga ttcagggttt cttcgacgtt
    29101 ccggttgata acgtatttgg tagcccgatc ctgctggaag acatgctgca gctgaatctg
    29161 gataacccaa ttgtggtttc tccggacatc ggcggcgttg tgcgtgcccg cgctatcgct
    29221 aagctgctga acgataccga tatggcaatc atcgacaaac gtcgtccgcg tgcgaacgtt
    29281 tcccaggtga tgcatatcat cggtgacgtt gcaggtcgtg actgcgtact ggtcgatgat
    29341 atgatcgaca ctggcggtac gctgtgtaaa gctgctgaag ctctgaaaga acgtggtgct
    29401 aaacgtgtat ttgcgtacgc gactcacccg atcttctctg gcaacgcggc gaacaacctg
    29461 cgtaactctg taattgatga agtcgttgtc tgcgatacca ttccgctgag cgatgaaatc
    29521 aaatcactgc cgaacgtgcg tactctgacc ctgtcaggta tgctggccga agcgattcgt
    29581 cgtatcagca acgaagaatc gatctctgcc atgttcgaac actaatcgaa cccggctcaa
    29641 agacccgctg cggcgggttt ttttgtctgt aatatccatt tgtatgacct atgcctcctt
    29701 cacctgccat ttagttgaca gatgatgcgc tcacggatga aacattattg tgaacaaaat
    29761 attttcctca catgtgatgc ctttccgcgc tctgatcgac gcttgctgga aagaaaaata
    29821 tactgccgca cggtttaccc gtgacctgat tgccgggata accgtcggga ttattgctat
    29881 cccgctggcg atggcgttgg ctattggtag tggtgtggcc ccccagtacg gtttatatac
    29941 cgcagctgtt gcggggattg tcattgctct gacggggggg tcacgcttta gcgtttccgg
    30001 tccgactgcg gcatttgtgg taattctcta tcccgtttcg caacagtttg gactggcagg
    30061 actgctggtt gcgaccttgc tgtcggggat ctttttgatt ctgatggggc tggcacgctt
    30121 tggtcgcctg attgagtata ttccggtttc cgtcacctta ggtttcacct cgggtatcgg
    30181 gatcaccatc ggtaccatgc agattaaaga ttttctcggt ctgcaaatgg cccatgtccc
    30241 ggaacattat ctacaaaaag tcggcgcatt atttatggcg ctgccgacca ttaatgtggg
    30301 tgatgcagcc attggcattg tgacgcttgg cattctggtt ttctggccgc gtctgggcat
    30361 acgtttaccc ggccaccttc cggccttgct ggctggttgc gcggtgatgg ggattgttaa
    30421 cctgctcggc agacatgttg ctaccatcgg ttcgcaattc cactacgtcc tggccgatgg
    30481 ttctcagggt aacggtattc cgcaactgct gccgcaactg gtgctgccgt gggatctgcc
    30541 taattcagaa ttcacgctaa cctgggattc tattcgcacc ctgctgcctg cggcattctc
    30601 aatggcaatg ctcggcgcaa tcgaatctct gctctgcgcc gtggtgctgg atggtatgac
    30661 cgggacgaaa cacaaggcga acagcgaact ggttggacag ggactgggga atattatcgc
    30721 tccgttcttt ggtggtatta ctgctacagc tgccatcgcg cgttctgccg ctaacgtccg
    30781 tgccggggca acttccccta tctcggcggt gatccactct attctggtta ttcttgccct
    30841 gctggtactg gcaccgctgc tctcctggct gccgctttcc gccatggcag ccctgctgtt
    30901 gatggtggcg tggaacatga gtgaagcgca caaagtggtc gacttgctgc gtcatgcacc
    30961 gaaagatgac atcatcgtca tgctgctgtg catgtcgctg accgtgctgt tcgatatggt
    31021 tattgccatc agcgtgggga tcgtgctggc atcgctgctg tttatgcgtc gtatcgcacg
    31081 tatgactcgc ctggcaccgg tagtcgtaga cgttccagac gatgtcctgg ttctgcgcgt
    31141 tattggcccg ctgttttttg ctgccgcaga aggcttattc acggacctgg agtcacgtct
    31201 tgaaggcaaa cggattgtga ttctgaagtg ggatgccgtt ccggtacttg atgctggtgg
    31261 tcttgatgcg ttccagcgtt ttgtgaagcg tctgcccgaa ggatgtgaac tgcgcgtgtg
    31321 caacctggaa ttccagccac tgcgcactat ggctcgcgca ggcattcaac cgatcccggg
    31381 acgcctcgca ttcttcccga atcgtcgcgc ggcgatggcg gatttataag tacgagattg
    31441 accagtcagc gcaaactgac tggtcagcaa actgcattat ttgttagcta tgacggcgat
    31501 tatcgctacg gcggcaacgt ttgtcatagt ggcgaaccca ccagtaacgg tcgcacacct
    31561 gttcatgccc gccaacacgg gcacctgcca gccagagaat cgcaccgacg aaaatactta
    31621 gcactgcacc atgtgcgaaa tactggggca tattaaactg tggtaactgg ttgaggattg
    31681 aataccccac gccgaccacc attaccacca gacccaaccc catgagcacg ttaccgagta
    31741 acgaagcgtt tttgcgtttc atatgtcacc tccggaactt tctgggttgt aacagggaat
    31801 acccctcttc cttatgtgta aagtatagac aacaccctgt aggttatgtg cgggtgtgat
    31861 cacaattaca ccccttattt caacaaaact ttacaaataa acgcctgaac accttgactt
    31921 tctaatagtc cactcaggca ataattgcgt tatctgcatt tgatacgcag ttttttgtca
    31981 agcggggccg caaccagtaa actacgcgcc agttatgtac acactcagga caaaaaaacg
    32041 tgacgattaa attgattgtc ggcctggcga accccggtgc tgaatacgcc gcaacgcgac
    32101 ataatgctgg tgcctggttc gttgacttac tggcagagcg attgcgcgct ccgctgcgcg
    32161 aagaggctaa attctttggt tatacttcgc gagtcaccct tggaggcgaa gatgtccgcc
    32221 tgttagtccc gactacattt atgaatctca gcggcaaagc cgttgcggcg atggccagtt
    32281 ttttccgcat taatccggac gaaattctgg tggcccacga cgaactggat ctgcctcctg
    32341 gcgtcgccaa atttaagttg ggtggcggtc atggtggtca caatggactg aaagacatca
    32401 tcagtaaatt gggtaataac cctaactttc accgtttacg catcggaatc ggtcatccgg
    32461 gcgataaaaa taaagttgtc ggttttgtgt taggcaaacc gcctgttagt gaacagaagt
    32521 taattgatga agccatcgac gaagcggcgc gttgtactga aatgtggttt acagatggct
    32581 tgaccaaagc aacgaaccga ttgcacgcct ttaaagcgca ataagtcgtt gtctgcggca
    32641 tttttgccga gtgccgtgta taataggcaa agttatttcc atttctgcaa tctgttagca
    32701 ataacaggtt gattattaag atattaaggt gatttaaatc atgggattca aatgcggtat
    32761 cgtcggtttg cccaacgtcg ggaaatctac cctgttcaac gcgctgacca aagccggtat
    32821 tgaagcggcc aactttccat tctgcaccat cgagccgaac acgggcgtcg taccaatgcc
    32881 cgaccctcgc ctggatcaac tggctgaaat cgtaaaaccg cagcgtacac ttcccacgac
    32941 catggaattt gtcgatatcg ccggtctggt aaaaggcgca tcgaaaggcg aaggtctggg
    33001 taaccagttc ctgaccaaca tccgtgaaac cgaagcgatc ggtcacgttg ttcgctgctt
    33061 tgaaaatgac aacatcattc acgtttccgg caaagttaac ccggcggatg atattgaagt
    33121 tatcaacacc gaactggcgc tggcggatct cgacacctgt gaacgtgcga ttcatcgcgt
    33181 acagaagaaa gccaaaggtg gcgataaaga cgcgaaagcc gagctggcgg tactggaaaa
    33241 atgcctgccc cagctggaaa acgcaggtat gctgcgtgcg ctggatttaa gcgctgaaga
    33301 gaaagcggct gttcgttacc tgagcttcct gacgctaaaa ccaacaatgt acatcgccaa
    33361 cgtcaacgaa gacggttttg aaaacaaccc gtatcttgac caggtgcgtg aaatcgcagc
    33421 gaaagaaggt tctgttgtag ttccggtttg tgctgctgtt gaagcagaca ttgccgaact
    33481 ggacgacgaa gagcgtgacg agtttatgca ggagcttggg ctggaagagc cgggcctgaa
    33541 ccgtgtgatc cgtgccggtt ataagctgct gaacctgcaa acttacttca ccgctggggt
    33601 gaaagaagtg cgtgcatgga ccattccggt tggtgcaacc gcgccgcagg cagcgggcaa
    33661 aatccatact gattttgaaa aaggctttat ccgtgcacaa accatctcgt ttgaagattt
    33721 catcacttac aaaggtgaac aaggcgcgaa agaagcgggc aaaatgcgtg cagaaggcaa
    33781 agattacatc gttaaagatg gcgatgtgat gaacttcctg ttcaacgtct aattccgcta
    33841 atactaccct ggcggttaat aacgagccat tatgtgagtc gcacataatg gcttttttac
    33901 gcctgctacg ctgcttcttt caattctgag ttaccgaaag tcataatcag actcctggca
    33961 atattaacaa tgcggctttt atgtgataac aatcacagtg cattaaccgt tcaagagttt
    34021 gtcatgaaaa aaacatttac cttcagcctg ttagcaattg tctttgcaag cctgttgagt
    34081 gcttgtgttc cccaccatca tcaccgccac agcgatggcc ctcgtggtcc tgcgccctcc
    34141 ggtaagccaa tgccaccttc aaatggtgga ccaaatcacg gcagtcatgg tcgctattaa
    34201 ttagcaattc atagcgggaa agtacatact ctcccgctat ccacggcgct taaactgccc
    34261 tgcatcaatg ccatgttgct tcattttccg ccacagcgta gtgcggccga ttccaagtaa
    34321 agccgacatt tcctgaatgc gaccgcctgt gacctgggct gcgttaataa ttgcctcttt
    34381 ttcaacttcc gcaaatgaca gactggtgga aaggcgggtg gcgctgacat catctgtcgc
    34441 ctgctcggta aacagatgtt ccggcaaatc actgacgcga atgcgcccat tatcactact
    34501 cagagcaaga ttctcgatga cgctgtaaag ttcaaaatcg ttgccaggcc atgcacagga
    34561 aaccaggcga gcgagggcat cgtcatcaat tttcagccgc gtagagaagc gtttttcaag
    34621 gctgcgtaat ttgttattca ccagcgccgg aatgctgcca cgccgcatac gcagtggcgg
    34681 gatggtaatt tcaaatgcat gcagcgcgta atacagctgg cgactaaaac gattttgttc
    34741 caccagcatt gcgaggtccg cggtcgttgt ggcaatcact ttgacatcaa tgggtattaa
    34801 acgccgcgca tccagtcgcg tgataacccc ctgcttgata acctgaagca aggcagactg
    34861 taactccacc gccagatatt caatcttttc aagaaacagc gtgccgccgt gcgccagttc
    34921 cagccgactc agacggccat tttcactatc cgtgcgatcg ccaccaataa attcttccgc
    34981 cagcgccgca tcaccatata actcacaatt gacggcgata taaggccccg ctgcacgctc
    35041 gctttcatta tgaattgcct gacttagcag tgccttgccc acgccctctt ctccacaaag
    35101 caggacagga aagctactgc gtgccgcctg gcgaccaaaa tgaatcaagc ggcgggtttg
    35161 cggatcgtcc tgtggcatat gagcgaaggt atggctgact tttcctaatt gactggtcat
    35221 taactgccgc atctgttcca caggatggag caataaaata aagctggttc cctgcgtttc
    35281 tattatcggt ttaagggtta tcaccgcatc aataaactga tgctgacttt caaaggtggc
    35341 ttctacgtgt ttgagcggat gtgcctgttt tattgcttgt tgcaatacgg cgggtaacgt
    35401 taagagttca gtgattgccc ttccctgact tgccgtcgcg tcaaggcgca agactcgcgc
    35461 cgcctgggcg ttgataaatt gcaaattacc ctgctcgtcc cagctaatca cgccatcatc
    35521 catactttct aacagggcat taagttgatt taaatgacgg ttagtttcag cgagcaaact
    35581 gtccgtcagc agtaaatttc cgacctcgcg tgcgattgcc aacgtcaacg gcaaatcagc
    35641 tgcggtagtt tgctcaaccg gacacgccag cgctattgtt cccgtcaatc ggcccttgct
    35701 gtcaaacaac ggcgttgcgc aaaaggccca gttccagagt gcctgtttga aatgttgatc
    35761 ggccatcgtt ttcacggcct gaccagagat agccgctaac gatagcgcac aagtaccaat
    35821 aattccctcg gcgcaatacg tgccgtcatt gaatcccagt acacttagct gctgcaaggt
    35881 ttgcggatcg ccattacggc tgagaatgca ggcggtttca tcgaggataa gcaacgcaca
    35941 ctctcgcggt gccatatatt cccaggcatc ttccagcgca gcctgcccga gcgtcagcat
    36001 cgctttctta cgccgataaa tagaagcaaa tgtcacgccc tgggcctggt gtggtacgtt
    36061 ccatgtctcc cgtttcatca gcttattgca tctctcccag gaggttgcaa ttaagggaga
    36121 tatgccccga tcatcgttgt taaaagcgcc actcatatcc cgcatccctt atgacaaaag
    36181 gtgttccggc tcgcaatttt aagtattaat acacgtaaaa gtttcaatat ggaacattca
    36241 ttaaagcata tgtttcacta tgaaacataa tttgggctaa ttttcttttc ccttgcctga
    36301 tgcacaatgg attcatcggc agtcagtggt cgccgtgtcg ttgaacatca tccatgccct
    36361 accgtaattg ctggagcaaa ataatgaaaa aattgatcaa tgatgtgcaa gacgtactgg
    36421 acgaacaact ggcaggacta gcgaaagcgc atccatcgct gacactgcat caggatccgg
    36481 tgtatgtcac ccgagctgat gcccccgttg caggaaaagt cgccctgctg tcgggtggcg
    36541 gcagcggaca cgagccgatg cattgtggtt atatcggtca ggggatgctt tctggggcct
    36601 gtccgggcga aattttcacc tcaccgacgc ccgataaaat ctttgaatgc gccatgcaaa
    36661 ttgatggcgg cgaaggtgta ctgttgatta tcaaaaatta caccggcgat attcttaact
    36721 tcgaaacagc aaccgagtta ctgcacgata gcggcgtaaa agtgaccact gtggtcattg
    36781 atgacgacgt tgcagtaaaa gacagtcttt ataccgccgg gcggcgcggc gttgccaaca
    36841 ccgtattaat tgaaaaactc gtaggtgcag cggcggagcg tggcgactca ctggacgcct
    36901 gtgcggaact ggggcgtaag ttgaataatc aaggccattc aataggtatc gctctcggtg
    36961 cctgtaccgt tcctgccgcg ggcaaacctt cttttaccct ggcggataat gagatggagt
    37021 ttggcgtcgg cattcatggt gaaccaggta ttgaccgccg ccccttctct tcccttgatc
    37081 aaaccgtcga tgaaatgttc gacaccctgc tggaaaatgg ctcataccat cgcactttgc
    37141 gtttctggga ttatcaacaa ggcagctggc aggaagaacc acaaaccaaa caaccgctcc
    37201 agtctggcga tcgggtgatt gcgctggtta acaatcttgg cgcaactccg ctttctgagc
    37261 tgtacggcgt ctataaccgc ctgaccacac gttgccagca agcgggattg actatcgaac
    37321 gtaatttaat tggcgcgtac tgcacctcac tggatatgac cggtttctca atcaccttac
    37381 tgaaagttga tgacgaaacg ctggcactct gggacgcccc tgtccacacc ccggccctta
    37441 actggggtaa ataaggagaa agcaatgtca ctgagcagaa ctcaaattgt taactggctc
    37501 actcgttgtg gcgatatttt cagcaccgag agcgagtatc ttactggact ggatcgcgag
    37561 attggcgacg ctgaccacgg gctaaatatg aatcgaggct ttagcaaagt ggtggaaaaa
    37621 ctccctgcta tcgcagataa agatatcggt ttcattctca agaataccgg catgacgcta
    37681 ctttccagcg tcggaggtgc cagtggtccg ctgttcggta ccttctttat ccgcgccgca
    37741 cagacaaccc aggcacggca aagcctgacg ctggaagagc tttatcagat gttccgcgat
    37801 ggcgcggacg gcgtaatcag tcgcgggaaa gccgaacctg gcgataaaac catgtgtgat
    37861 gtgtgggtgc cggtggtgga atcgttacgt cagtcctgca agcaaaatct ctctgttccg
    37921 gcggcgctcg acgctgccag tagcatcgcc gaatccgctg cacaaagtac gattacgatg
    37981 caagcccgca aaggccgcgc cagttatctc ggtgaacgca gtattggtca ccaggatccc
    38041 ggcgcgacct cggtgatgtt tatgatgcaa atgttggcgt tagccgcaaa agagtaagga
    38101 attggtgatg gtaaacctgg tcatagtttc acatagcagc cgactgggag aaggtgtcgg
    38161 tgaattagcc cgtcagatgt taatgagtga taattgtaaa atcgccattg ccgcgggaat
    38221 tgacgatcca caaaatccca ttggtaccga tgccgtcaaa gtgatggagg ccatcgaatc
    38281 tgttgccgat gccgaccatg tgctggtcat gatggatatg ggtagcgcat tattgagtgc
    38341 tgaaactgcg ctggaattgc tggctcccga gatcgccgca aaagtacgtt tgtgtgctgc
    38401 gccgttggtc gaaggtacac tggcagcaac ggtcagcgcg gcctcggggg cggatatcga
    38461 caaagttatc tttgacgcca tgcatgcgct ggaagccaaa cgtgaacaac tgggtttacc
    38521 gtcctccgac actgaaatct ctgacacatg tcctccgtac gatgaagaag cccgttctct
    38581 gtcggtggtc ataaaaaacc gtaacggcct gcatgtacgt ccggcctccc ggctggttta
    38641 taccttatcg acatttaatg ccgatatgtt gctggagaaa aacggcaaat gcgttacacc
    38701 ggagagtatt aaccagattg cgttactaca agttcgctat aacgatacgc tgcgcctgat
    38761 tgcgaaagga ccagaagctg aagaggcact gatcgctttc cgtcagctgg ctgaagataa
    38821 ctttggtgaa acggaggaag ttgctccacc tactctgcgt cccgttccgc ctgtttcggg
    38881 taaagccttt tattatcaac ctgttttatg tacggtacag gcaaaatcaa ccctgaccgt
    38941 ggaagaagaa caagaacgat tacgccaggc cattgacttc acgttattag atctgatgac
    39001 gttaacagcg aaagcagaaa cctgcgggct tgacgatatt gccgcaatct tttctggtca
    39061 ccatacactg ttagatgatc cggaactgca ggcggcggca agcgaactcc ttcagcatga
    39121 acattgcacg gcggaatatg cctggcagca agttcttaaa gaacttagcc agcaatacca
    39181 acagctggat gatgaatatc tacaggctcg ctatatcgat gtggacgatc ttctgcatcg
    39241 caccctggtc cacctgaccc aaacgaaaga agaactcccg cagtttaact cgccaactat
    39301 tctactggcg gagaacattt atccctccac agtactgcaa ctggatccag cggttgtaaa
    39361 aggtatctgc cttagcgccg gaagtccgct atcccacagt gccctaatcg cccgtgaact
    39421 gggaattggc tggatttgcc agcagggtga gaaactgtat gcgatacaac cagaagaaac
    39481 gctaacgctg gacgttaaaa cgcaacgttt caaccgtcag ggttaaacca ccgttcggat
    39541 ggcatcattc tgatgtcatc cggcaattta ctctgcactg atgtttttta actacctgtt
    39601 atatttttca ccaaattatg atgatttttc ttcgctaatc gcgaacccaa cgtcgctggc
    39661 tgacgaataa acagatgtta ttttatcact ctatttcgcc gttatttatc cctttaattc
    39721 ccttttctaa aatgtctgtc agttcgcaga atgtgatttc gatcatgcag ctagtgcgat
    39781 cctgaactaa ggttttctga tacttgaata ccgttttatt cggtttcgcc aaaggagaat
    39841 gactgatgaa atcccccgca ccttctcgcc cgcaaaaaat ggcgttaatt ccagcctgca
    39901 tctttttgtg tttcgctgcg ctatcggtgc aggcagaaga aacatcagta acaccacaac
    39961 cgcctgatat tttattaggg ccgctgttta atgatgtgca aaacgccaaa ctttttccgg
    40021 accaaaaaac ctttgccgat gccgtgccga acagcgatcc gctgatgatc cttgctgatt
    40081 atcggatgca gcaaaaccag agcggatttg atctacgcca tttcgttaac gtcaatttca
    40141 ccctgccgaa agagggcgag aaatatgttc cgccagaggg gcaatcactg cgcgaacata
    40201 ttgacggact ttggctggta ttaacgcgtt ctaccgaaaa caccgaaaaa tgggattctc
    40261 tattaccgct gccaaaacct tatgtcgtgc caggcggacg ttttcgcgag gtatattact
    40321 gggacagtta cttcaccatg ttaggacttg ccgaaagcgg tcactgggat aaagtcgcgg
    40381 atatggtggc caattttgct catgaaatag acaattacgg tcatattccc aacggcaacc
    40441 gcagttacta tttaagccgc tcgcaaccgc ccttctttgc cctgatggta gagttactgg
    40501 cgcagcatga aggcgacgcc gcgttgaaac aatacctgcc gcaaatgcaa aaagaatatg
    40561 cttactggat ggacggtgtt gaaaacctgc aagccggaca acaggaaaaa cgcgttgtaa
    40621 aacttcagga tggtaccctt ctcaaccgct actgggacga tcgcgatacg ccacgaccag
    40681 agtcatgggt ggaagatatt gccactgcca aaagcaatcc gaatcgacct gccactgaaa
    40741 tttaccgcga cctgcgctct gccgctgcgt ctggctggga tttcagctcg cgctggatgg
    40801 acaatccgca gcagttaaac accttacgca ccaccagcat cgtaccggtc gatctgaaca
    40861 gcttgatgtt caaaatggaa aaaatcctcg cccgcgccag caaagctatc ggagataacg
    40921 cgatggcaaa ccagtacgaa acgctggcaa atgcccgtca aaaagggatc gaaaaatacc
    40981 tgtggaacga tcaacaaggc tggtatgccg attacgacct gaaaagtcat aaagtgcgca
    41041 atcagttaac cgcggccgcc ctgttcccgc tgtacgtcaa tgcggcagcg aaagatcgcg
    41101 ccagcaaaat ggcgacggcg acgaaaacac atctgctgca accaggcggc ctgaacacca
    41161 cgtcggtgaa aagtgggcaa caatgggatg cgccaaacgg ctgggcaccg ttgcagtggg
    41221 tcgcgactga aggattacaa aactacgggc aaaaagaggt agcgatggac attagctggc
    41281 acttcctgac caatgttcag cacacctatg accgggagaa aaagctggtg gaaaaatatg
    41341 atgtcagcac caccggaaca gggggcggcg gtggcgaata tccattacag gatggctttg
    41401 gctggaccaa tggcgtgacg ctgaaaatgc tggatttgat ctgcccgaaa gagcaaccgt
    41461 gtgacaatgt tccggcgacg cgtccactca gtgaatcaac aacacagccg ctaaaacaaa
    41521 aagaggcgga acctacgcct taactttact gtatatcgag caatgccatt acgtgcataa
    41581 aattggcatt gctctttctt catcacgaaa taccaaat
//
LOCUS       DADSYM010000017        41290 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2415
            SAMN08039579-rid11723173.denovo.017, whole genome shotgun sequence.
ACCESSION   DADSYM010000017 DADSYM010000000
VERSION     DADSYM010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039579
            Sequence Read Archive: SRR6362533
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 41290)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 41290)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980162.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 03:39:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,484
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 4,347
            CDSs (with protein)               :: 4,347
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 43 of 85
            Pseudo Genes (incomplete)         :: 37 of 85
            Pseudo Genes (internal stop)      :: 19 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41290
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039579-rid11723173.denovo.017"
                     /strain="LGE2415"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8302 N 95.9628 W"
                     /collection_date="2015-08-24"
     gene            127..630
                     /gene="dps"
                     /locus_tag="J1Y83_000792"
                     /gene_synonym="pexB"
     CDS             127..630
                     /gene="dps"
                     /locus_tag="J1Y83_000792"
                     /gene_synonym="pexB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001730644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein Dps"
                     /protein_id="HBA7628970.1"
                     /translation="MSTAKLVKSKATNLLYTRNDVSDSEKKATVELLNRQVIQFIDLS
                     LITKQAHWNMRGANFIAVHEMLDGFRTALIDHLDTMAERAVQLGGVALGTTQVINSKT
                     PLKSYPLDIHNVQDHLKELADRYAIVANDVRKAIGEAKDDDTADILTAASRDLDKFLW
                     FIESNIE"
     gene            1034..1780
                     /gene="glnH"
                     /locus_tag="J1Y83_000793"
     CDS             1034..1780
                     /gene="glnH"
                     /locus_tag="J1Y83_000793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter substrate-binding
                     protein GlnH"
                     /protein_id="HBA7628971.1"
                     /translation="MKSVLKVSLAALTLAFAVSSHAADKKLVVATDTAFVPFEFKQGD
                     KYVGFDVDLWAAIAKELKLDYELKPMDFSGIIPALQTKNVDLALAGITITDERKKAID
                     FSDGYYKSGLLVMVKANNNDVKSVKDLDGKVVAVKSGTGSVDYAKANIKTKDLRQFPN
                     IDNAYMELGTNRADAVLHDTPNILYFIKTAGNGQFKAVGDSLEAQQYGIAFPKGSDEL
                     RDKVNGALKTLRENGTYNEIYKKWFGTEPK"
     gene            1919..2578
                     /gene="glnP"
                     /locus_tag="J1Y83_000794"
     CDS             1919..2578
                     /gene="glnP"
                     /locus_tag="J1Y83_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015367581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter permease GlnP"
                     /protein_id="HBA7628972.1"
                     /translation="MQFDWSAIWPAIPLLIEGAKMTLWISVLGLAGGLVIGLLAGFAR
                     TFGGWIANHVALVFIEVIRGTPIVVQVMFIYFALPMAFNDLRIDPFTAAVVTIMINSG
                     AYIAEITRGAVLSIHKGFREAGLALGLSRWETIRYVILPLALRRMLPPLGNQWIISIK
                     DTSLFIVIGVAELTRQGQEIIAGNFRALEIWSAVAVFYLIITLVLSFILRRLERRMKI
                     L"
     gene            2575..3297
                     /gene="glnQ"
                     /locus_tag="J1Y83_000795"
     CDS             2575..3297
                     /gene="glnQ"
                     /locus_tag="J1Y83_000795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017491583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter ATP-binding protein
                     GlnQ"
                     /protein_id="HBA7628973.1"
                     /translation="MIEFKNVSKHFGPTQVLHNIDLNIAQGEVVVIIGPSGSGKSTLL
                     RCINKLEEITSGDLIVDGLKVNDPKVDERLIRQEAGMVFQQFYLFPHLTALENVMFGP
                     LRVRGANKEEAEKLARELLAKVGLAERAHHYPSELSGGQQQRVAIARALAVKPKMMLF
                     DEPTSALDPELRHEVLKVMQDLAEEGMTMVIVTHEIGFAEKVASRLIFIDKGRIAEDG
                     DPQVLIKNPPSQRLQEFLQHVS"
     gene            3414..5639
                     /gene="ybiO"
                     /locus_tag="J1Y83_000796"
     CDS             3414..5639
                     /gene="ybiO"
                     /locus_tag="J1Y83_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415329.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive channel protein"
                     /protein_id="HBA7628974.1"
                     /translation="MRWILFILFCLLGAPAHAVSIPGVTTTTTTDSTTEPAPEPDIEQ
                     KKAAYGALADVLDNDTSRKELIDQLRTVAATPPAEPVPKIVPPTLVEEQTVLQKVTEV
                     SRHYGEALSARFGQLYRNITGSPHKPFNPQTFSNALTHFSMLAVLVFGFYWLIRLCAL
                     PLYRKMGQWARQKNRERSNWLQLPAMIIGAFIIDLLLLALTLFVGQVLSDNLNAGSRT
                     IAFQQSLFLNAFALIEFFKAVLRLIFCPNVAELRPFTIHDESARYWSRRLSWLSSLIG
                     YGLIVAVPIISNQVNVQIGALANVIIMLCMTVWALYLIFRNKKEITQHLLNFAEHSLA
                     FFSLFIRAFALVWHWLASAYFIVLFFFSLFDPGNSLKFMMGATVRSLAIIGIAAFVSG
                     MFSRWLAKTITLSPHTQRNYPELQKRLNGWLSAALKTARILTVCVAVMLLLSAWGLFD
                     FWNWLQNGAGQKTVDILIRIALILFFSAVGWTVLASLIENRLASDIHGRPLPSARTRT
                     LLTLFRNALAVIISTITIMIVLSEIGVNIAPLLAGAGALGLAISFGSQTLVKDIITGV
                     FIQFENGMNTGDLVTIGPLTGTVERMSIRSVGVRQDTGAYHIIPWSSITTFANFVRGI
                     GSVVANYDVDRHEDADKANQALKDAVAELMENEEIRGLVIGEPNFAGIVGLSNTAFTL
                     RVSFTTLPLKQWTVRFALDSQVKKHFDLAGVRAPVQTYQVLPAPGAAPAEPLPPGEPT
                     L"
     gene            complement(5636..6562)
                     /gene="rlmF"
                     /locus_tag="J1Y83_000797"
     CDS             complement(5636..6562)
                     /gene="rlmF"
                     /locus_tag="J1Y83_000797"
                     /EC_number="2.1.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415328.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(1618)-N(6))-methyltransferase
                     RlmF"
                     /protein_id="HBA7628975.1"
                     /translation="MSAQKPGLHPRNRHHSRYDLATLCQVNPELRQFLTLTPAGEQSV
                     DFANPLAVKALNKALLAHFYAVANWDIPDGFLCPPVPGRADYIHHLADLLAEASGTIP
                     ANASILDIGVGANCIYPLIGVHEYGWRFTGSETSSQALSSAQAIISANPGLNRAIRLR
                     RQKESGAIFNGIIHKNEQYDATLCNPPFHDSAAAARAGSERKRRNLGLNKDDALNFGG
                     QQQELWCEGGEVAFIKKMIEESKGFAKQVMWFTSLVSRGENLPPLYRALTDVGAVKVV
                     KKEMAQGQKQSRFIAWTFMNDEQRRRFVNRQR"
     gene            6838..7098
                     /locus_tag="J1Y83_000798"
     CDS             6838..7098
                     /locus_tag="J1Y83_000798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415327.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HBA7628976.1"
                     /translation="MKKCLTLLIATVLSGISLTAYAAQPMSNLDRGQLRPAGTVSATG
                     ASNLSDLEDKLAEKAREQGAKGYVINSAGGNDQMFGTAIIYK"
     gene            7363..9645
                     /locus_tag="J1Y83_000799"
     CDS             7363..9645
                     /locus_tag="J1Y83_000799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catecholate siderophore receptor Fiu"
                     /protein_id="HBA7628977.1"
                     /translation="MENNRNFPARQFHSLTFFAGLCIGITPVAQALAAEGQANADDTL
                     VVEASTPSLYAPQQSADPKFSRPVADTTRTMTVISEQVIKDQGATNLTDALKNVPGVG
                     AFFAGENGNSTTGDAIYMRGADTSNSIYIDGIRDIGSVSRDTFNTEQVEVIKGPSGTD
                     YGRSAPTGSINMISKQPRNDSGIDASASIGSAWFRRGTLDVNQVIGDTTAVRLNVMGE
                     KTHDAGRDKVKNERYGVAPSVAFGLGTANRLYLNYLHVTQHNTPDGGIPTIGLPGYSA
                     PSAGTAALNHSGKVDTHNFYGTDSDYDDSTTDTATMRFEHDINDNTTIRNTTRWSRVK
                     QDYLMTAIMGGASNITQPTSDVNSWTWSRTANTKDVSNKILTNQTNLTSTFYTGAIGH
                     DLSTGVEFTRETQTNYGVNPVTLPAVNIYHPDSSIHPGGLTRNGANANGQTDTFAIYA
                     FDTLQITRDFELNGGIRLDNYHTEYDSATACGGSGRGAITCPAGVAKGSPVTTVDTAK
                     SGNLVNWKAGALYHLTENGNVYINYAVSQQPPGGNNFALAQSGSGNSANRTDFKPQKA
                     NTSEIGTKWQVLDKRLLLTAALFRTDIENEVEQNDDGTYSQYGKKRVEGYEISVAGNI
                     TPAWQMIGGYTQQKATIKNGKDVAQDGSSSLPYTPEHAFTLWSQYQATDDISVGAGAR
                     YIGSMHKGSDGAVGTPAFTEGYWVADAKLGYRVNRNLDFQLNVYNLFDTDYVASINKS
                     GYRYHPGEPRTFLLTANMHF"
     gene            9861..10538
                     /gene="ybiX"
                     /locus_tag="J1Y83_000800"
     CDS             9861..10538
                     /gene="ybiX"
                     /locus_tag="J1Y83_000800"
                     /EC_number="1.14.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKHD-type hydroxylase YbiX"
                     /protein_id="HBA7628978.1"
                     /translation="MMYHIPGVLSPQDVARFREQLEQAEWVDGRLTTGAQGAQVKNNQ
                     QVDTRSALYAALQNEVLNAVNQHALFFAAALPRTLSTPLFNRYQNNETYGFHVDGAVR
                     SHPQNGWMRTDLSATLFLSDPESYDGGELVVNDTFGQHRVKLPAGDLVLYPSSSLHCV
                     TPVTRGVRVASFMWIQSMIRDDKKRAMLFELDNNIQSLKSHYGESEEILSLLNLYHNL
                     LREWSEI"
     gene            10612..10878
                     /gene="ybiI"
                     /locus_tag="J1Y83_000801"
     CDS             10612..10878
                     /gene="ybiI"
                     /locus_tag="J1Y83_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-type zinc finger protein YbiI"
                     /protein_id="HBA7628979.1"
                     /translation="MASGWANDDAVNEQINSTIEDAIARARGEIPRGESLYECEECGA
                     PIPQARREAIPGVRLCIHCQQEKDLQKPDYTGYNRRDSKDSQLR"
     gene            11143..11403
                     /locus_tag="J1Y83_000802"
     CDS             11143..11403
                     /locus_tag="J1Y83_000802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HBA7628980.1"
                     /translation="MKTINTVVAAMALSTLSFGVFAAEPVTASQAQNMNKIGVVSADG
                     ASTLDALEAKLAEKAAAAGASGYSITSATNNNKLSGTAVIYK"
     gene            complement(11631..12716)
                     /locus_tag="J1Y83_000803"
     CDS             complement(11631..12716)
                     /locus_tag="J1Y83_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate/lactate/ureidoglycolate dehydrogenase"
                     /protein_id="HBA7628981.1"
                     /translation="MVSGHRFDAQTLHSFIQAVFRQMGSEEQEAKLVADHLIAANLAG
                     HDSHGIGMIPSYVRSWSQGHLQINHHAKVVKEAGAAVTLDGDRAFGQVAAHEAMALGI
                     EKARQHGIAAVALHNSHHIGRIGYWAEQCAAAGFVSIHFVSVVGIPMVAPFHGRDSRF
                     GTNPFCVVFPRKDNFPLLLDYATSAIAFGKTRVAWHKGVPVPPGCLIDVNGVPTTNPA
                     VMQESPLGSLLTFAEHKGYALAAMCEILGGALSGGKTTHQETLQTSPDAILNCMTTII
                     INPELFGAPDCSAQTEAFAEWVKASPHDDDKPILLPGEWEVNTRRERQEQGIPLDAGS
                     WQAICDAARQIGMPEETLQAFCQQLAS"
     gene            complement(12857..13819)
                     /gene="ybiB"
                     /locus_tag="J1Y83_000804"
     CDS             complement(12857..13819)
                     /gene="ybiB"
                     /locus_tag="J1Y83_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein YbiB"
                     /protein_id="HBA7628982.1"
                     /translation="MDYRKIIKEIGRGKNHARDLDRDTARGLYAHMLNGEVPDLELGG
                     VLIALRIKGEGEAEMLGFYEAMQNHTIKLTPPAGKPMPIVIPSYNGARKQANLTPLLA
                     ILLHKLGFPVVVHGVSEDPTRVLTETIFELMGITPTLHGGQAQAKLDEHQPVFMPVGA
                     FCPPLEKQLAMRWRMGVRNSAHTLAKLATPFAEGEALRLSSVSHPEYIGRVAKFFSDI
                     GGRALLMHGTEGEVYANPQRCPQINLIDREGMRVLYEKQDTAGSELLPQAKDPETTAQ
                     WIERCLAGSEPIPESLKIQMACCLMATGEAATISDGLARVNQAF"
     gene            complement(13847..15997)
                     /gene="dinG"
                     /locus_tag="J1Y83_000805"
     CDS             complement(13847..15997)
                     /gene="dinG"
                     /locus_tag="J1Y83_000805"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase DinG"
                     /protein_id="HBA7628983.1"
                     /translation="MALTAALKAQIAAWYKALQEQIPDFIPRAPQRQMIADVAKTLAG
                     EEGRHLAIEAPTGVGKTLSYLIPGIAIAREEQKTLVVSTANVALQDQIYSKDLPLLKK
                     IIPDLKFTAAFGRGRYVCPRNLTALASTEPTQQDLLAFLDDELTPNNQEEQKRCAKLK
                     GDLDTYKWDGLRDHTDIAIDDDLWRRLSTDKASCLNRNCYYYRECPFFVARREIQEAE
                     VVVANHALVMAAMESEAVLPDPKNLLLVLDEGHHLPDVARDALEMSAEITAPWYRLQL
                     DLFTKLVATCMEQFRPKTIPPLAIPERLNAHCEELYELIASLNNILNLYMPAGQEAEH
                     RFAMGELPDEVLEICQRLAKLTEMLRGLAELFLNDLSEKTGSHDIVRLHRLILQMNRA
                     LGMFEAQSKLWRLASLAQSSGAPVTKWATREEREGQLHLWFHCVGIRVSDQLERLLWR
                     SIPHIIVTSATLRSLNSFSRLQEMSGLKEKAGDRFVALDSPFNHCEQGKIVIPRMRVE
                     PSIDNEEQHIAEMAAFFREQVESKKHLGMLVLFASGRAMQRFLDYVTDLRLMLLVQGD
                     QPRYRLVELHRKRVANGERSVLVGLQSFAEGLDLKGDLLSQVHIHKIAFPPIDSPVVI
                     TEGEWLKSLNRYPFEVQSLPSASFNLIQQVGRLIRSHGCWGEVVIYDKRLLTKNYGKR
                     LLDALPVFPIEQPEVPEGIVKKKEKTKSPRRRRR"
     gene            16101..16301
                     /locus_tag="J1Y83_000806"
                     /pseudo
     CDS             16101..16301
                     /locus_tag="J1Y83_000806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706676.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1768 domain-containing protein"
     gene            complement(16534..17895)
                     /gene="rhlE"
                     /locus_tag="J1Y83_000807"
     CDS             complement(16534..17895)
                     /gene="rhlE"
                     /locus_tag="J1Y83_000807"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase RhlE"
                     /protein_id="HBA7628984.1"
                     /translation="MSFDSLGLSPDILRAVAEQGYREPTPIQQQAIPAVLEGRDLMAS
                     AQTGTGKTAGFTLPLLQHLITRQPHAKGRRPVRALILTPTRELAAQIGENVRDYSKYL
                     NIRSLVVFGGVSINPQMMKLRGGVDVLVATPGRLLDLEHQNAVKLDQVEILVLDEADR
                     MLDMGFIHDIRRVLTKLPAKRQNLLFSATFSDDIKALAEKLLHNPLEIEVARRNTASD
                     QVTQHVHFVDKKRKRELLSHMIGKGNWQQVLVFTRTKHGANHLAEQLNKDGIRSAAIH
                     GNKSQGARTRALADFKSGDIRVLVATDIAARGLDIEELPHVVNYELPNVPEDYVHRIG
                     RTGRAAATGEALSLVCVDEHKLLRDIEKLLKKEIPRIAIPGYEPDPSIKAEPIQNGRQ
                     QRGGGRGQGGGRGQQQPRRTESGAKSGNAKPAEKPSRRLGDAKPAGEQQRRRRPRKPA
                     AAQ"
     gene            18124..18795
                     /gene="cecR"
                     /locus_tag="J1Y83_000808"
     CDS             18124..18795
                     /gene="cecR"
                     /locus_tag="J1Y83_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415317.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CecR"
                     /protein_id="HBA7628985.1"
                     /translation="MNNPAMTIKGEQAKKQLIAAALAQFGEYGMNATTREIAAQAGQN
                     IAAITYYFGSKEDLYLACAQWIADFIGEQFRPHAEEAERLFAQPQPDRAAIRELILRA
                     CKNMIKLLTQDDTVNLSKFISREQLSPTAAYHLVHEQVISPLHSHLTRLIAAWTGCDA
                     NDTRMILHTHALIGEILAFRLGKETILLRTGWTAFDEEKTELINQTVTCHIDLILQGL
                     SQRSL"
     gene            18795..19793
                     /gene="hlyD"
                     /locus_tag="J1Y83_000809"
     CDS             18795..19793
                     /gene="hlyD"
                     /locus_tag="J1Y83_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretion protein HlyD"
                     /protein_id="HBA7628986.1"
                     /translation="MMKKTVVIGLAVVVLAAVVAGGYWWYQSRQDNGLTLYGNVDIRT
                     VNLSFRVGGRVESLAVDEGDAIKAGQVLGELDHKPYEIALMQAKAGVSVAQAQYDLML
                     AGYRDEEIAQAAAAVKQAQAAYDYAQNFYNRQQGLWKSRTISANDLENARSSRDQAQA
                     TLKSAQDKLRQYRSGNREQDIAQAKASLEQAQAQLAQAELNLQDSTLVAPSDGTLLTR
                     AVEPGTVLNEGGTVFTVSLTRPVWVRAYVDERNLDQAQPGRKVLLYTDGRPNKPYHGQ
                     IGFVSPTAEFTPKTVETPDLRTDLVYRLRIVVTDADDALRQGMPVTVQFGEEAGHE"
     gene            19786..21522
                     /locus_tag="J1Y83_000810"
     CDS             19786..21522
                     /locus_tag="J1Y83_000810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415315.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBA7628987.1"
                     /translation="MNDAVITLNGLEKRFPGMDKPAVAPLDCTIHAGYVTGLVGPDGA
                     GKTTLMRMLAGLLKPDSGSATVIGFDPIKNDGALHAVLGYMPQKFGLYEDLTVMENLN
                     LYADLRSVTGEARKQTFARLLEFTSLGPFTGRLAGKLSGGMKQKLGLACTLVGEPKVL
                     LLDEPGVGVDPISRRELWQMVHELAGEGMLILWSTSYLDEAEQCRDVLLMNEGELLYQ
                     GEPTALTQTMAGRSFLMTSPHEGNRKLLQRALKLPQVSDGMIQGKSVRLILKKEATPD
                     DIRHADGMPEININETTPRFEDAFIDLLGGAGTSESPLGAILHTVEGTPGETVIEAKE
                     LTKKFGDFAATDHVNFAVKRGEIFGLLGPNGAGKSTTFKMMCGLLVPTSGQALVLGMD
                     LKESSGKARQHLGYMAQKFSLYGNLTVEQNLRFFSGVYGLRGRAQNEKISRMSEAFGL
                     KSIASHATDELPLGFKQRLALACSLMHEPDILFLDEPTSGVDPLTRREFWLHINSMVE
                     KGVTVMVTTHFMDEAEYCDRIGLVYRGKLIASGTPDDLKAQSANDEQPDPTMEQAFIQ
                     LIHDWDKEHSNE"
     gene            21515..22648
                     /locus_tag="J1Y83_000811"
     CDS             21515..22648
                     /locus_tag="J1Y83_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBA7628988.1"
                     /translation="MSNPILSWRRVRALCVKETRQIIRDPSSWLIAVVIPLLLLFIFG
                     YGINLDSSKLRVGILLEQRSEAALDFTHTMTGSPYIDATISDNRQELIAKMQAGKIRG
                     LVVIPVDFAEQMERANATAPIQVITDGSEPNTANFVQGYVEGIWQIWQMQRAEDNGQT
                     FEPLIDVQTRYWFNPAAISQHFIIPGAVTIIMTVIGAILTSLVVAREWERGTMEALLS
                     TEITRTELLLCKLIPYYFLGMLAMLLCMLVSVFILGVPYRGSLLILFFISSLFLLSTL
                     GMGLLISTITRNQFNAAQVALNAAFLPSIMLSGFIFQIDSMPAVIRAVTYIIPARYFV
                     STLQSLFLAGNIPVVLIVNVLFLIASAVMFIGLTWLKTKRRLD"
     gene            22659..23765
                     /locus_tag="J1Y83_000812"
     CDS             22659..23765
                     /locus_tag="J1Y83_000812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBA7628989.1"
                     /translation="MFHRLWTLIRKELQSLLREPQTRAILILPVLIQVILFPFAATLE
                     VTNATIAIYDEDNGEHSVELTQRFARASAFTHVLLLKSPQEIRPTIDTQKALLLVRFP
                     ADFSRKLDTFQTAPLQLILDGRNSNSAQIAANYLQQIVKNYQQELLEGKPKPNNSELV
                     VRNWYNPNLDYKWFVVPSLIAMITTIGVMIVTSLSVAREREQGTLDQLLVSPLTTWQI
                     FIGKAVPALIVATFQATIVLAIGIWAYQIPFAGSLALFYFTMVIYGLSLVGFGLLISS
                     LCSTQQQAFIGVFVFMMPAILLSGYVSPVENMPMWLQNLTWINPIRHFTDITKQIYLK
                     DASLDIVWNSLWPLLVITATTGSAAYAMFRRKVM"
     gene            complement(23727..24137)
                     /locus_tag="J1Y83_000813"
     CDS             complement(23727..24137)
                     /locus_tag="J1Y83_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA7628990.1"
                     /translation="MKWQQRVRVATGLSCWQIMLHLLVVALLVVGWMSKTLVHVGVGL
                     CALYCVTVVMMLVFQRHPEQRWREVADVLEELTTTWYFGAALIVLWLLSRVLENNFLL
                     AIAGLAILAGPAVVSLLAKDKKLHHLTSKHRVRR"
     gene            24270..25031
                     /locus_tag="J1Y83_000814"
     CDS             24270..25031
                     /locus_tag="J1Y83_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HBA7628991.1"
                     /translation="MPDQTQQFSFKVLTINIHKGFTAFNRRFILPELRDAVRTVSADI
                     VCLQEVMGAHEVHPLHVENWPDTSHYEFLADTMWSDFAYGRNAVYPEGHHGNAVLSRY
                     PIEHYENRDVSVDGAEKRGVLYCRIVPPMTGKAIHVMCVHLGLREAHRQAQLAMLAEW
                     VNELPDGEPVLVAGDFNDWRQKANHPLKVQAGLDEIFTRAHGRPARTFPVQFPLLRLD
                     RIYVKNASASAPTALPLRTWRHLSDHAPLSAEIHL"
     gene            25028..26269
                     /gene="clsB"
                     /locus_tag="J1Y83_000815"
     CDS             25028..26269
                     /gene="clsB"
                     /locus_tag="J1Y83_000815"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase ClsB"
                     /protein_id="HBA7628992.1"
                     /translation="MKCSWREGNKIQLLENGEQYYPAVFKAIGEAQERIILETFIWFE
                     DDVGKQLHAALLAAAQRGVKAEVLLDGYGSPDLSDEFVNELTAAGVVFRYYDPRPRLF
                     GMRTNVFRRMHRKIVVIDAHIAFIGGLNYSAEHMSSYGPEAKQDYAVRLEGPIVEDIL
                     QFELENLPGQSAARRWWRRHHKAEENRQPGEAQVLLVWRDNEEHRDDIERHYLKMLTQ
                     ARREVIIANAYFFPGYRFLHALRKAARRGVRIKLIIQGEPDMPIVRVGARLLYNYLVK
                     GGVQVFEYRRRPLHGKVALMDDHWATVGSSNLDPLSLSLNLEANVIIHDRNFNQTLRD
                     NLNGIIAADCQQIDETMLPKRTWWNLTKSVLAFHFLRHFPALVGWLPAHTPRLAQVDP
                     PAQPTMETQDRVETENTGVKP"
     gene            26269..27225
                     /locus_tag="J1Y83_000816"
     CDS             26269..27225
                     /locus_tag="J1Y83_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0104 family protein"
                     /protein_id="HBA7628993.1"
                     /translation="MSKSHPRWRLAKKILTWLFFIAVIVLLVVYAKKVDWEEVWKVIR
                     DYNRVALLSAVGLVVVSYLIYGCYDLLARFYCGHKLAKRQVMLVSFICYAFNLTLSTW
                     VGGIGMRYRLYSRLGLPGSTITRIFSLSITTNWLGYILLAGVIFTAGVVELPDHWYVD
                     QTTLRILGIGLLMIIAVYLWFCAFAKHRHMTIKGQKLVLPSWKFALAQMLISSVNWMV
                     MGAIIWLLLGQSVNYFFVLGVLLVSSIAGVIVHIPAGIGVLEAVFITLLAGEHTSKGT
                     IIAALLAYRVLYYFIPLLLALICYLLLESQAKKLRAKNEAAM"
     gene            complement(27261..27974)
                     /locus_tag="J1Y83_000817"
     CDS             complement(27261..27974)
                     /locus_tag="J1Y83_000817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HBA7628994.1"
                     /translation="MESYSQNSNKLDFQHEARILNGIWLITALGLVTTAGLAWGAKYL
                     EITATKYDSPQMYVAIGLLLLSMYGLSKDINKINTAIAGVIYLFLISLVAIVVASLAP
                     VSAIIVVFSTAGSMFLISMLAGLLFKFDPGSHRFIIMMTLTGLALVIIVNATLMSERP
                     IWVISCLMIVLWSGIISHGRNKLLELTGKSHSEELWSPVRCAFVGALTLYYYFIGFFG
                     ILAAIAITLVWQRHTRFFH"
     gene            complement(28179..28883)
                     /locus_tag="J1Y83_000818"
     CDS             complement(28179..28883)
                     /locus_tag="J1Y83_000818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HBA7628995.1"
                     /translation="MDRFPRSDSIVQPRAGLQTYMAQVYGWMTVGLLLTAFVAWYAAN
                     SAAVMELLFTNRVFLIGLIIAQLALVIVLSAMIQKLSAGVTTMLFMLYSALTGLTLSS
                     IFIVYTAASIASTFVVTAGMFGAMSLYGYTTKRDLSGFGNMLFMALIGIVLASLVNFW
                     LKSEALMWAVTYIGVIVFVGLTAYDTQKLKNMGEQIDTRDTSNLRKYSILGALTLYLD
                     FINLFLMLLRIFGNRR"
     gene            complement(29020..29472)
                     /gene="moaE"
                     /locus_tag="J1Y83_000819"
     CDS             complement(29020..29472)
                     /gene="moaE"
                     /locus_tag="J1Y83_000819"
                     /EC_number="2.8.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin synthase catalytic subunit MoaE"
                     /protein_id="HBA7628996.1"
                     /translation="MAETKIVVGPQPFSVGEEYPWLAERDEDGAVVTFTGKVRNHNLG
                     DSVKALTLEHYPGMTEKALAEIVDEARNRWPLGRVTVIHRIGELWPGDEIVFVGVTSA
                     HRSSAFEAGQFIMDYLKTRAPFWKREATPEGDRWVEARESDQQAAKRW"
     gene            complement(29474..29719)
                     /gene="moaD"
                     /locus_tag="J1Y83_000820"
     CDS             complement(29474..29719)
                     /gene="moaD"
                     /locus_tag="J1Y83_000820"
                     /EC_number="2.8.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin synthase sulfur carrier subunit"
                     /protein_id="HBA7628997.1"
                     /translation="MINVLFFAQVRELVGTDATEVAADFPTVEALRQHLAAQSDRWAL
                     ALEDGKLLAAVNQTLVSFDHSLTDGDEVAFFPPVTGG"
     gene            complement(29712..30197)
                     /gene="moaC"
                     /locus_tag="J1Y83_000821"
     CDS             complement(29712..30197)
                     /gene="moaC"
                     /locus_tag="J1Y83_000821"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HBA7628998.1"
                     /translation="MSQLTHINAAGEAHMVDVSAKAETVREARAEAFVTMRSETLAMI
                     IDGRHHKGDVFATARIAGIQAAKRTWDLIPLCHPLMLSKVEVNLQAEPEHNRVRIETL
                     CRLTGKTGVEMEALTAASVAALTIYDMCKAVQKDMVIGPVRLLAKSGGKSGDFKVEAD
                     D"
     gene            complement(30200..30712)
                     /gene="moaB"
                     /locus_tag="J1Y83_000822"
     CDS             complement(30200..30712)
                     /gene="moaB"
                     /locus_tag="J1Y83_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein B"
                     /protein_id="HBA7628999.1"
                     /translation="MSQVSTEFIPTRIAILTVSNRRGEEDDTSGHYLRDSAQEAGHHV
                     VDKAIVKENRYAIRAQVSAWIASDDVQVVLITGGTGLTEGDQAPEALLPLFDREVEGF
                     GEVFRMLSFEEIGTSTLQSRAVAGVANKTLIFAMPGSTKACRTAWENIIAPQLDARTR
                     PCNFHPHLKK"
     gene            complement(30734..31723)
                     /gene="moaA"
                     /locus_tag="J1Y83_000823"
     CDS             complement(30734..31723)
                     /gene="moaA"
                     /locus_tag="J1Y83_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HBA7629000.1"
                     /translation="MASQLTDAFARKFYYLRLSITDVCNFRCTYCLPDGYKPSGVTNK
                     GFLTVDEIRRVTRAFASLGTEKVRLTGGEPSLRRDFTDIIAAVRENNTIRQIAVTTNG
                     YRLERDVANWRDAGLTGINVSVDSLDARQFHAITGQDKFNQVMAGIDAAFEAGFEKVK
                     VNTVLMRDVNHHQLDTFLNWIQHRPIQLRFIELMETGEGSELFRKHHISGQVLRDELL
                     RRGWIHQLRQRSDGPAQVFCHPDYAGEIGLIMPYEKDFCATCNRLRVSSIGKLHLCLF
                     GEGGVNLRDLLEDDTQQQALEARISAALREKKQTHFLHQNNTGITQNLSYIGG"
     regulatory      complement(31711..31855)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01055"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="molybdenum cofactor riboswitch; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="molybdenum cofactor"
                     /db_xref="RFAM:RF01055"
     gene            32120..33028
                     /gene="yvcK"
                     /locus_tag="J1Y83_000824"
     CDS             32120..33028
                     /gene="yvcK"
                     /locus_tag="J1Y83_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine diphosphate-N-acetylglucosamine-binding
                     protein YvcK"
                     /protein_id="HBA7629001.1"
                     /translation="MRNRTLADLDRVVALGGGHGLGRVLSSLSSLGSRLTGIVTTTDN
                     GGSTGRIRRSEGGIAWGDMRNCLNQLITEPSVASAMFEYRFGGNGELSGHNLGNLMLK
                     ALDHLSVRPLEAINLIRNLLKVDAHLIPMSEHPVDLMAIDDQGHEVYGEVNIDQLTAP
                     IQELLLTPNVPATREAVHAISEADLIIIGPGSFYTSLMPILLLKEIAQALRRTPAPMV
                     YIGNLGRELSLPAANLKLESKLAIMEQYVGKKVIDAVIVGPKEDVSAVKERIVIQEVL
                     EASDIPYRHDRQLLHSALEKALQALG"
     gene            complement(33066..35087)
                     /gene="uvrB"
                     /locus_tag="J1Y83_000825"
     CDS             complement(33066..35087)
                     /gene="uvrB"
                     /locus_tag="J1Y83_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit B"
                     /protein_id="HBA7629002.1"
                     /translation="MSKPFKLNSAFKPSGDQPEAIRRLEEGLEDGLAHQTLLGVTGSG
                     KTFTIANVIADLQRPTMVLAPNKTLAAQLYGEMKEFFPENAVEYFVSYYDYYQPEAYV
                     PSSDTFIEKDASVNEHIEQMRLSATKAMLERRDVVVVASVSAIYGLGDPDLYLKMMLH
                     LTVGMIIDQRAILRRLAELQYARNDQAFQRGTFRVRGEVIDIFPAESDDIALRVELFD
                     EEVERLSLFDPLTGQIVSTIPRFTIYPKTHYVTPRERIVQAMEEIKEELAARRKVLLE
                     NNKLLEDQRLTQRTQFDLEMMNELGYCSGIENYSRFLSGRGPGEPPPTLFDYLPADGL
                     LVVDESHVTIPQIGGMYRGDRARKETLVEYGFRLPSALDNRPLKFEEFEALAPQTIYV
                     SATPGNYELEKSGGDVVDQVVRPTGLLDPIIEVRPVATQVDDLLSEIRQRAAINERVL
                     VTTLTKRMAEDLTEYLEEHGERVRYLHSDIDTVERMEIIRDLRLGEFDVLVGINLLRE
                     GLDMPEVSLVAILDADKEGFLRSERSLIQTIGRAARNVNGKAILYGDKITPSMAKAIG
                     ETERRREKQQKYNEEHGITPQGLNKKVVDILALGQNIAKTKAKGRGKSRPIVEPDNVP
                     MDMSPKALQQKIHELEGLMMQHAQNLEFEEAAQIRDQLHQLRELFIAAS"
     gene            complement(35666..36343)
                     /gene="bioD"
                     /locus_tag="J1Y83_000826"
     CDS             complement(35666..36343)
                     /gene="bioD"
                     /locus_tag="J1Y83_000826"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent dethiobiotin synthetase BioD"
                     /protein_id="HBA7629003.1"
                     /translation="MSKCYFVTGTDTEVGKTVASCALLQAAKAAGYRTAGYKPVASGS
                     EKTPKGLRNSDALALQRNSSLQLDYATVNPYTFAEPTSPHIVSAQEGRPIESSVMSAG
                     LRALEQQADWVLVEGAGGWFTPLSDTFTFADWVTQEQLPVILVVGVKLGCINHAMLTA
                     QAIQHAGLTLAGWVANDVTPPGKRHAEYMTTLTRMIPAPLLGEIPWLAENPENAATGK
                     YINLALM"
     gene            complement(36336..37091)
                     /gene="bioC"
                     /locus_tag="J1Y83_000827"
     CDS             complement(36336..37091)
                     /gene="bioC"
                     /locus_tag="J1Y83_000827"
                     /EC_number="2.1.1.197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonyl-ACP O-methyltransferase BioC"
                     /protein_id="HBA7629004.1"
                     /translation="MATVNKQAIAAAFGRAAAHYEQHADLQRQSADALLAMLPQRKYT
                     HVLDAGCGPGWMSRRWRERHAQVTALDLSPPMLVQARQKDAADHYLAGDIESLPLATA
                     TFDLAWSNLAVQWCGNLSTALRELYRVVRPGGVVAFTTLVQGSLPELHQAWQAVDERP
                     HANRFLPPDKIEQSLNAVHYQHYIQPITLWFDDALSAMRSLKGIGATHLHEGRDPRIL
                     TRSQLQRLQLAWPQQQGRYPLTYHLFLGVIARE"
     gene            complement(37078..38232)
                     /gene="bioF"
                     /locus_tag="J1Y83_000828"
     CDS             complement(37078..38232)
                     /gene="bioF"
                     /locus_tag="J1Y83_000828"
                     /EC_number="2.3.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-amino-7-oxononanoate synthase"
                     /protein_id="HBA7629005.1"
                     /translation="MIWQEKIDAALDARRVADALRRRYPVAQGAGRWLVADDCQYLNF
                     SSNDYLGLSHHPQIIRAWQQGAEQFGVGSGGSGHVSGYSVAHQVLEEELAEWLGYSRA
                     LLFISGFAANQAVIAAMMAKEDRIVADRLSHASLLEAASLSPSPLRRFAHNDVTHLAR
                     LLASPCPGQQLVVTEGVFSMDGDSAPLEEIQQVTQQHDGWLMVDDAHGTGVIGEQGRG
                     SCWLQKVKPELLVVTFGKGFGVSGAAVLCSNTVADYLLQFARHLIYSTSMPPAQAQAL
                     RASLAVIRSDEGDARREKLAALITRFRAGVQDLPFTLADSWSAIQPLIVGDNSRALQL
                     AEKLRQQGCWVTAIRPPTVPAGTARLRLTLTAAHEMQDIDRLLEVLHGNG"
     gene            complement(38229..39269)
                     /gene="bioB"
                     /locus_tag="J1Y83_000829"
     CDS             complement(38229..39269)
                     /gene="bioB"
                     /locus_tag="J1Y83_000829"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HBA7629006.1"
                     /translation="MAHRLRWTLSQVTELFEKPLLDLLFEAQQVHRQHFDPRQVQVST
                     LLSIKTGACPEDCKYCPQSSRYKTGLEAERLMEVEQVLESARKAKAAGSTRFCMGAAW
                     KNPHERDMPYLEQMVQGVKAMGLEACMTLGTLSESQAQRLANAGLDYYNHNLDTSPEF
                     YGNIITTRTYQERLDTLEKVREAGIKVCSGGIVGLGETVKDRAGLLLQLTNLPTPPES
                     VPINMLVKVKGTPLADNDDVDAFDFIRTIAVARIMMPTSYVRLSAGREQMNEQTQAMC
                     FMAGANSIFYGCKLLTTPNPEEDKDLQLFRKLGLNPQQTAVLAGDNEQQQRLEQALMT
                     PDTDEYYNAAAL"
     gene            39356..40645
                     /gene="bioA"
                     /locus_tag="J1Y83_000830"
     CDS             39356..40645
                     /gene="bioA"
                     /locus_tag="J1Y83_000830"
                     /EC_number="2.6.1.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine--8-amino-7-oxononanoate
                     transaminase"
                     /protein_id="HBA7629007.1"
                     /translation="MTTDDLTFDQRHIWHPYTSMTSPLPVYPVASAEGCELILSDGRR
                     LVDGMSSWWAAIHGYNHPQLNAAMKSQIDAMSHVMFGGITHAPAIELCRKLVAMTPQP
                     LECVFLADSGSVAVEVAMKMALQYWQAKGEARQRFLTFRNGYHGDTFGAMSVCDPDNS
                     MHSLWKGYLPENLFAPAPQSRMDGEWDERDMVGFARLMAAHRHEIAAVIIEPIVQGAG
                     GMRMYHPEWLKRIRKMCDREGILLIADEIATGFGRTGKLFACEHAEIAPDILCLGKAL
                     TGGTMTLSATLTTREVAETISNGEAGCFMHGPTFMGNPLACAAANASLAILESGDWQH
                     QVAAIEAQLREQLAPACDAEMVADVRVLGAIGVVETTRPVNMAALQKFFVEQGVWIRP
                     FGKLIYLMPPYIILPQQLQRLTAAVNRAVQDETFFCQ"
     gene            40704..41180
                     /locus_tag="J1Y83_000831"
     CDS             40704..41180
                     /locus_tag="J1Y83_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase inhibitor"
                     /protein_id="HBA7629008.1"
                     /translation="MKLISNDLRDGDKLPHRHVFNGMGYDGDNISPHLAWDDVPVGTK
                     SFVVTCYDPDAPTGSGWWHWVVVNLPADTRVLPQGFGSGLVAMPDGVLQTRTDFGKTG
                     YDGAAPPKGETHRYIFTVHALDVERIDVDEGASGAMVGFNVHFHSLASASITAMFS"
BASE COUNT         9700 a        11050 c        10699 g         9841 t
ORIGIN      
        1 ttttgttttt tcacgcttgt taccactatt agtgtgatag gaacagccag aatagcggaa
       61 cacatagctg gtgctatact tagtctcgtt aattactggg acataacatc aagaggatat
      121 gaaattatga gtaccgctaa attagttaaa tcaaaagcga ccaatctgct ttatacccgc
      181 aacgatgtct ctgacagcga gaaaaaagca acagtagagt tgctgaatcg ccaggttatc
      241 cagtttattg atctttcttt gattaccaaa caagcgcact ggaacatgcg cggcgctaac
      301 ttcattgccg tacatgaaat gctggatggc ttccgcactg cactgatcga tcatctggat
      361 accatggcag aacgtgcagt gcagctgggc ggtgtagctc tggggaccac tcaagttatc
      421 aacagcaaaa ctccgctgaa aagttacccg ctggacatcc acaacgttca ggatcacctg
      481 aaagagctgg ctgaccgtta cgcaatcgtc gctaatgacg tacgcaaagc gattggcgaa
      541 gcgaaagatg acgacaccgc agatatcctg accgccgcgt ctcgcgacct ggataaattc
      601 ctgtggttta tcgagtctaa catcgaataa atccatcgct gatggtgcag aactttagta
      661 cacgataaaa gcggcttcct gacaggaggc cgttttgttt tgtagcccgc ctcaacgcac
      721 ttatttagtg catccatctg ctatcaccag ctggttaagt aaattttttg tatccacatc
      781 atcacacaat cgttacataa agattgtttt ttcatcaggt tttacgctaa ataatcactg
      841 tgttgagtgc acaattttag cgcaccagtt tggtgcccca gaatggtgca tcttcagggt
      901 attgccctat aaatcgtgca tcacgttttt accgcatctc gataaatcaa tgagttgcaa
      961 aactggcacg attttttcat atatgtgaat gtcacgcagg ggatcgtccc gtggatagaa
     1021 aaaaggaaat gctatgaagt ctgtattaaa agtttcactg gctgcactga ccctggcttt
     1081 tgcggtttct tctcacgccg cggataaaaa attagttgtc gcgacggata ctgccttcgt
     1141 tccgtttgaa tttaaacagg gcgataaata cgtgggcttt gacgttgatc tgtgggctgc
     1201 catcgctaaa gagctgaagc tggattacga actgaagccg atggatttca gtgggatcat
     1261 tccggcactg caaaccaaaa acgtcgatct ggcgctggcg ggcatcacca ttaccgacga
     1321 gcgtaaaaaa gcgatcgatt tctctgacgg ctactacaaa agcggcctgt tagtgatggt
     1381 gaaagctaac aataacgatg tgaaaagcgt gaaagatctc gacgggaaag tggttgctgt
     1441 gaagagcggc actggctccg ttgattacgc gaaagcaaac atcaaaacta aagatctgcg
     1501 tcagttcccg aacattgata acgcctatat ggaactgggc accaaccgcg cagatgccgt
     1561 tctgcacgat acgccaaaca ttttgtactt catcaaaacc gccggtaacg gtcagttcaa
     1621 agcggtaggt gactctctgg aagcacagca atacggtatt gcgttcccga aaggtagcga
     1681 cgagctgcgt gacaaagtca acggcgcgtt gaaaaccctg cgcgagaacg gaacttacaa
     1741 cgaaatctac aaaaaatggt tcggtactga accgaaataa taacgctacg cctgtaaagc
     1801 gcactggcag ttccctctcc cctctgggga gaggattagg gtgaggggcg caaacccgct
     1861 ccgaggccat taattaccct gaatttgatt atttacacca cggtaacagg aacaacatat
     1921 gcagtttgac tggagtgcca tctggcctgc cattccgctt ctgattgaag gtgccaaaat
     1981 gaccctgtgg atttcggtcc tcggtttggc tggcggtctg gtgatcggat tgctggcagg
     2041 ttttgcacgc accttcggtg gttggatagc caaccacgtc gcgctggtct ttattgaagt
     2101 gatccgcggc acacctatcg tcgtccaggt gatgtttatc tatttcgccc tgccgatggc
     2161 gtttaacgac ttacgcatcg acccgtttac tgcggcggtg gtcaccatca tgatcaactc
     2221 cggcgcgtac attgcggaaa tcacgcgtgg tgcggtgctg tctattcaca aaggttttcg
     2281 tgaagcagga ctggcgctcg gtctttcacg ttgggaaacc attcgctacg tcattttacc
     2341 gctggcactg cgtcgtatgc tgccgccgct gggtaaccag tggatcatca gtataaaaga
     2401 tacctcgctg tttattgtta tcggcgtggc ggaactgacc cgtcaggggc aagaaattat
     2461 tgccggtaac ttccgcgccc ttgagatctg gagcgccgtg gcggtgttct atctgattat
     2521 taccctggtg ctgagcttta ttctgcgtcg tctggaaaga aggatgaaaa tcctgtgatt
     2581 gaatttaaaa acgtctccaa gcattttggt ccaacccagg tgctgcacaa tatcgatttg
     2641 aacattgctc agggcgaagt cgtggtgatt atcgggccgt ccggttccgg taaatcgacc
     2701 ctgctgcgct gcatcaacaa actggaagaa atcacctccg gcgatctgat tgtcgatggc
     2761 ctgaaggtta acgatccgaa agttgatgag cgcctgattc gccaggaagc gggtatggtg
     2821 ttccagcagt tttacctctt cccgcatctg acggcgctgg aaaacgtcat gtttggcccg
     2881 ctacgcgtgc gtggcgcgaa taaagaagag gcggaaaaac tggcgcgtga gctgctggcg
     2941 aaagtcggtc tggcagagcg tgcacatcac tacccttccg aactttctgg tggtcaacag
     3001 cagcgtgtgg cgattgcccg cgcgctggcg gtgaagccga aaatgatgct gtttgatgaa
     3061 ccgacttccg ctcttgaccc ggaactgcgc catgaagtgc tgaaggttat gcaggatctg
     3121 gctgaagaag ggatgacgat ggtgatcgtg acccacgaaa tcggttttgc cgagaaagta
     3181 gcttcgcgcc tgatctttat cgacaaaggc cggattgcag aagatggcga tccgcaggtg
     3241 ttgatcaaga acccgccgag ccagcgcttg caggaatttt tgcagcacgt ctcttaactt
     3301 ctctgcctcc ctttcccccg aaagggaggc acaccagatt cctctcattt aaaatcgccc
     3361 agcctccagc atctatactt atctttttgc tctattttct cactggagga gtcatgcggt
     3421 ggatcctgtt catcctcttc tgcctgttgg gcgcacctgc ccacgcggta tccatacccg
     3481 gcgttacaac cacaacgaca acggactcaa cgactgaacc ggccccggaa ccggatatcg
     3541 aacaaaaaaa agcggcctat ggcgcactgg cggatgtgct ggataatgac acctcgcgta
     3601 aagagttgat cgaccagttg cgcaccgtcg ccgctacgcc tcctgcggaa cccgtaccga
     3661 agatcgtgcc gccgacgctg gttgaagagc aaaccgtgct gcaaaaagtc accgaagtca
     3721 gccgccatta tggtgaagcc ctttccgccc gcttcgggca actttatcgc aatatcaccg
     3781 gctccccgca taagccgttt aatccacaaa ccttcagcaa tgcgctgacc cacttttcaa
     3841 tgttagcggt attagtgttt ggtttttact ggctgattcg tctgtgcgca ctgccgctgt
     3901 atcgcaaaat gggccagtgg gcgcggcaaa aaaatcgtga gcgcagtaac tggttgcagc
     3961 ttccggcgat gatcatcggg gcgtttatta tcgacctgct gttactggca ctgacattat
     4021 ttgtcggcca ggtattaagc gacaacctga atgcgggcag tcgcaccatc gctttccaac
     4081 aaagtttgtt tctcaacgcc tttgccctga ttgaattttt caaagccgta ctacgcctga
     4141 ttttttgccc aaacgtggct gaactgcgcc cgttcacgat tcatgacgag agcgcccgtt
     4201 actggagtcg tcgcctgagc tggttaagca gcctgattgg ctatggcctg attgtggctg
     4261 tgccgattat ctctaatcag gtgaatgtac agataggtgc gctggcgaac gtcatcatta
     4321 tgctgtgcat gaccgtctgg gcgttgtacc tgatttttcg taataaaaaa gagatcaccc
     4381 agcatttgct caactttgcg gagcattcgc tggccttttt cagcctgttt atccgcgcct
     4441 ttgcgctggt gtggcactgg ctggcaagcg cctattttat cgtgctgttt ttcttttcgt
     4501 tgttcgatcc gggcaacagc ctgaaattta tgatgggtgc aacggtgcgc agcctggcga
     4561 ttattggtat cgcagcattt gtttccggta tgttttcccg ctggttggcg aaaaccatca
     4621 ccctctcgcc acatactcag cgtaactatc ccgagctgca aaaacggctg aatggatggc
     4681 tttcggcggc actgaaaaca gcgcgtattc tgacagtctg cgtggcggta atgctgctgt
     4741 tgagcgcatg gggattgttc gatttctgga actggctgca aaacggagcg gggcagaaaa
     4801 ccgtagatat cctgatccgt atcgcactca ttcttttctt ctcggcggtt ggctggacgg
     4861 tgctcgccag tttgatagaa aaccggctgg cttcggatat tcatggccgc ccgctaccca
     4921 gcgcccgtac gcgaaccctg ctgacgctgt tccgtaacgc gctggcggtg attatcagta
     4981 ccatcaccat catgattgtg ttgtcggaaa tcggcgtcaa tatcgcgcca ttgctggcag
     5041 gtgccggggc attaggtctg gctatctcgt ttggttcgca aacgctggtg aaagatatta
     5101 ttaccggagt atttattcag tttgaaaacg gcatgaacac tggagatttg gtgactatcg
     5161 ggccgttgac cggcactgtg gaacggatgt cgattcgctc cgtgggcgtg cgccaggata
     5221 ccggggcgta tcacatcatt ccgtggtctt cgataaccac ctttgctaac ttcgtccgcg
     5281 gtattggttc ggtagtggca aactacgatg ttgatcgcca tgaagatgct gataaagcca
     5341 atcaggcact gaaagatgcg gtagcagaat tgatggaaaa cgaagaaatt cgcgggctgg
     5401 ttattggtga accgaacttt gccgggattg tcggcttaag caataccgcg tttacactgc
     5461 gtgtttcgtt caccacgctg ccactcaaac agtggacggt ccgctttgcc ctcgacagcc
     5521 aggtgaaaaa acatttcgac ctggcgggtg ttcgcgcgcc ggtgcagact tatcaggtgc
     5581 tgccagctcc gggcgcggcc ccggctgaac cgttgccgcc gggggagcca acgctttaac
     5641 gctggcgatt gacaaaacgg cggcgctgct cgtcgttcat aaaggtccag gctataaagc
     5701 ggctctgctt ttgcccctgg gccatctctt tttttaccac cttcaccgca cctacgtccg
     5761 tcagagcacg atacaacggc ggtaagtttt caccacgaga taccagcgag gtaaaccaca
     5821 tcacctgctt cgcgaagcct ttgctctctt caatcatctt tttgataaaa gcgacctcac
     5881 cgccttcaca ccacaactcc tgttgctgac caccaaagtt cagtgcatcg tctttgttca
     5941 gccccaggtt acggcgttta cgttcactac ctgcccgtgc cgcagcggcg gaatcgtgga
     6001 atggcgggtt acacaaggtc gcatcgtatt gctcgttttt atggatgatg ccgttaaaaa
     6061 tcgccccact ctctttttgc cgacgcagac gaatagcgcg gttaagcccc ggattagcac
     6121 tgatgatcgc ctgcgcactg cttaacgcct ggctgctggt ttcactaccg gtaaaacgcc
     6181 agccatattc atgtacgcca atcagcggat aaatacagtt cgcaccaacg ccgatatcca
     6241 gaatgctggc attagctgga attgttccgc ttgcttcagc cagtaaatcg gcaaggtgat
     6301 gaatataatc cgcccggccc ggtactggcg ggcagagaaa accgtccggg atatcccagt
     6361 tcgctacggc gtaaaaatgg gccagcaacg ccttattgag cgccttcacc gccagcggat
     6421 tggcaaagtc tacgctttgc tccccggcgg gtgtaagcgt gaggaattgc ctgagttcag
     6481 gattgacctg acaaagcgtg gcgagatcgt agcggctgtg atgacggttg cgcggatgca
     6541 accccggttt ctgggcggac atagcattct cctttaagca gcggcgtaag atacccgcat
     6601 aacgcgacag ggtaaatatc tggctgcgtt tatcatttct aaaaaccttc acatggctat
     6661 ggtttatggc aattcaccat aaaaatcatc atcatgcatg tcattgattt gtcttatttt
     6721 gtaaaaacat atcgttcaaa aaataagcac atctctgtca attttttacg tcattttcat
     6781 cgcgcaggcg acctacactt atctggcact acccgttgta tttatgagga tgtcattatg
     6841 aaaaagtgcc tcacactact gattgccacc gtcctgagcg gaatctctct cacggcttat
     6901 gccgcgcaac cgatgagtaa cctggacaga ggtcaactac ggcccgccgg taccgtttcg
     6961 gcaactggcg catcaaacct aagcgatctg gaggataaac tggcagaaaa agcgcgcgaa
     7021 caaggcgcga aaggttatgt cattaattcc gcaggcggaa atgaccagat gttcggtact
     7081 gcaatcatct acaaataacc ccaccaacgt aactgcattc gtctctggat gcagttttcg
     7141 tccgatttgt aagcaaaaaa catcaaatat gaacgcaatg taaataaatg tatttctttt
     7201 tcgcgcaatg ggtgatagaa aatcgcgcca agtgataatg cttatcaaaa ttattatcac
     7261 ttgcacgtac gctatcacgg gattaacagt ggcatcgcat ccgcagagat gctttctcgt
     7321 gacagtgaaa atttcaccat ataagaaaaa gtcacctgca aaatggaaaa caatcgcaat
     7381 ttccctgcca gacaatttca ttcgctcacg ttctttgccg gtctttgtat tggtattacg
     7441 cctgtggcac aggcactcgc tgccgaaggg caagctaacg cggatgacac gctggttgtc
     7501 gaagcatcaa cgccttcgct ttatgcgcca caacaatctg ccgatccgaa attctcgcgt
     7561 ccggtagcgg atactacccg cacgatgacg gtgatttctg aacaagtgat taaagatcag
     7621 ggtgcaacca accttaccga cgcgctcaaa aacgtccccg gcgtgggtgc gttttttgcg
     7681 ggtgagaacg gtaactccac cactggcgac gccatttata tgcgcggtgc cgatacctct
     7741 aacagtattt atatcgatgg cattcgcgat atcggcagcg tctcgcgcga caccttcaat
     7801 accgagcagg tcgaagtaat taaagggccg tccggcaccg actacgggcg cagcgcgccg
     7861 acaggctcga tcaatatgat cagcaaacag ccgcgcaatg attccggcat tgacgcctcc
     7921 gccagtattg gcagcgcctg gttccgccgc ggcacgctgg acgtcaatca ggtcattggt
     7981 gataccactg cggtgcgcct gaatgtaatg ggcgaaaaaa cgcacgatgc cggacgcgac
     8041 aaagtcaaaa atgagcgtta cggcgtcgcc ccttctgtcg cttttggcct tggtacagcg
     8101 aatcgtttgt atcttaatta tctgcatgtc acccagcaca acacgccaga cggcggcatt
     8161 ccgactatcg gtttaccggg ttattctgcc ccatctgcgg gaacagcagc cctgaatcat
     8221 tccggaaaag ttgatactca taacttttac ggcacagatt ccgattacga cgattcgacc
     8281 accgacaccg ctaccatgcg ttttgagcac gacatcaacg ataacaccac cattcgcaac
     8341 actacgcgtt ggtcgcgcgt aaagcaggat tacctgatga cggcgattat gggtggagcg
     8401 tcgaatatta cccagcccac cagcgatgtg aatagctgga cgtggtcacg cacggcgaat
     8461 accaaagatg tgagtaacaa aattctcacc aaccagacca acctgacctc gacgttctat
     8521 accggcgcta tcggtcatga tctcagtacc ggcgtggaat ttacccgcga aacacagact
     8581 aactatggcg ttaatccggt gacgttaccg gcggtaaata tttatcatcc tgacagcagc
     8641 attcatcccg gcggcctgac gcgcaacggc gcaaacgcca atggtcagac ggataccttc
     8701 gcaatttatg ccttcgatac gctgcaaatc acccgtgatt ttgagctgaa cggcgggatc
     8761 cgtctggata attatcatac tgaatatgac agtgctaccg cctgcggcgg cagcgggcgc
     8821 ggtgccatca cctgccctgc tggcgtggca aaaggttctc cggtcaccac cgtcgacacc
     8881 gccaagtcgg gcaatctggt gaactggaaa gccggggcgc tgtatcacct gacggaaaac
     8941 ggcaatgtct atattaacta tgccgtttcc cagcagccgc cgggcggcaa caacttcgcc
     9001 cttgcgcagt ctggcagcgg taacagtgcc aaccgcaccg attttaaacc acaaaaagcc
     9061 aacaccagcg agattggcac caaatggcag gttctggata aacgcctgct acttaccgcc
     9121 gcgctgttcc gtactgatat tgaaaatgaa gttgagcaaa atgatgacgg gacttattcg
     9181 caatatggta agaaacgtgt cgaaggctac gagatatccg tggccgggaa tatcactccc
     9241 gcgtggcaga tgattggcgg ctatacccag caaaaagcaa ctatcaaaaa cggcaaagat
     9301 gttgcccagg atggttcctc atcgctgccg tataccccgg aacacgcctt caccttatgg
     9361 agccagtatc aggcaaccga tgatatttct gttggtgcgg gcgcacggta tatcggcagt
     9421 atgcataaag gttcagacgg cgcggtgggg acgccagcat ttaccgaagg ttattgggtc
     9481 gccgacgcca aactggggta tcgggttaat cgcaatctcg acttccagct aaacgtctac
     9541 aacctgtttg ataccgatta cgtcgcctca atcaataaga gcggctaccg ttatcacccg
     9601 ggcgagccaa gaaccttctt gctcacagct aatatgcatt tctgattcag atgtggggcg
     9661 caaggctcac taatgttgat ggcgagagca ggactgcctg atgcgctgcg cttatcaggc
     9721 ctacaaggtt gtgcaataca ctgaattcac atcatttggt aggccggata aggcgttcac
     9781 gccgcatccg gcacctgagc accattgcct gctgttaaaa atcggtgtgg ggcgcaggcc
     9841 ccactttttg gagaacttgt atgatgtacc acattcccgg cgtgttatcg ccacaggacg
     9901 tcgcccgttt tcgcgaacaa ctggaacaag ccgaatgggt ggatgggcgc ctcaccaccg
     9961 gcgcacaagg tgcgcaagtt aagaacaatc aacaagtaga cacccgcagc gccctgtacg
    10021 ccgccctgca aaatgaggtg ctgaacgcgg ttaaccaaca tgctttgttc tttgccgcgg
    10081 ccttgccgcg taccctttcc acgccgctgt ttaatcgcta tcagaacaat gaaacctatg
    10141 gttttcatgt ggatggcgca gtacgcagcc atccacaaaa cggctggatg cgcactgacc
    10201 tttctgccac gctgttttta agcgatccag aaagctacga cggcggcgaa ctggtcgtta
    10261 atgacacctt cgggcaacat cgggtaaaac tcccggcagg cgatctggtg ttgtatccct
    10321 ccagcagcct gcattgcgtg acacccgtaa cccgcggcgt gcgagtggca tcatttatgt
    10381 ggatccagtc gatgatccgc gatgataaaa agcgcgccat gctgtttgaa ctggacaaca
    10441 atattcagtc gctgaaaagc cactacggcg aaagtgaaga gatcctgtcg ctgctcaatc
    10501 tttatcataa tctgctgcgg gaatggtcgg agatctgacg cttgaactgc ccgagttcgt
    10561 ccctatatta gctacactta actgtacaag cattgatatg gggaggacga tatggcatcc
    10621 ggttgggcta atgatgacgc cgtcaacgaa cagatcaaca gtacaattga agatgcgatt
    10681 gcccgcgctc ggggtgaaat tccgcgtggc gaaagcctgt atgaatgtga agagtgcggt
    10741 gcccctatcc cgcaggcccg tcgggaagcc attcctggcg tgcgcttatg cattcattgt
    10801 cagcaggaga aagatttaca aaaaccagat tatacaggat ataatcgcag agattcgaaa
    10861 gacagccagt tacgttaact acaactggcg ggatgtatag cgaatataat ttggattttg
    10921 cgtggcattc cgtgccttta acgctaccgt gactgccacg cggtataaaa caagtccata
    10981 agtacaacaa ataaatggtt tatcagtatg ttagatatca atcaatttat ttgaacaagg
    11041 cggtcaattc tcttcgattt tatctctcga aaaaaaacgt gatactcatc acatcgacga
    11101 aacaacgtca cttatacaaa aatcacctgc gagagattaa ttatgaaaac tatcaatact
    11161 gttgttgctg ctatggctct ttcaactctg tcatttggcg tgttcgcggc ggaaccggta
    11221 acggcatccc aggcacagaa catgaataaa atcggcgtgg tttctgccga tggcgcatcc
    11281 accctcgatg ccctggaagc gaaactggct gagaaagccg cggcggccgg tgccagtgga
    11341 tacagcatca cttctgccac caacaacaat aaattaagcg gtactgcggt aatttataag
    11401 taattcgtag gacggataag gcgtttacgc cgcatccggc aacagatgcc tgatgcgacg
    11461 ctaccgcgtc ttatcagacc tacaaaatcc gaaccgttgg ctggatcagg cgtttacgct
    11521 acgttcagca caagtatttc cctgtctgtt tgccgacaga cgcatatgct ctaaccctca
    11581 ttgatcctat gttacccttg tttgcccgtc cgccactgga cgggcttttt ttagctggct
    11641 aactgctgac agaaagcctg caacgtctct tccggcatac caatctgccg cgctgcatca
    11701 caaatggcct gccagcttcc cgcatccagt ggaatcccct gctcctgccg ttcgcgacgc
    11761 gtgttcactt cccactcgcc cggtagcaaa atcggcttat cgtcatcatg cggcgaggct
    11821 ttcacccact cggcaaaggc ttcggtctgt gcactacaat ccggcgcgcc gaacagttcc
    11881 gggttgataa tgatagtggt catgcagttg agaatggcat cgggactggt ttgtaacgtt
    11941 tcctgatgcg tcgttttacc gccagaaagc gccccgccaa gaatttcaca catcgctgca
    12001 agggcgtagc ctttatgttc ggcaaaggtc aacagcgaac ccaacggcga ctcctgcatt
    12061 accgccggat ttgtcgtcgg cacgccgtta acgtcaatca ggcaacctgg cggcacgggg
    12121 acgcctttat gccaggcgac gcgggttttg ccaaatgcaa tggcgctggt ggcgtaatcg
    12181 agcaacagcg ggaaattatc tttacgaggg aaaaccacac agaacggatt ggtgccaaag
    12241 cggctgtcgc gaccgtggaa cggcgcgacc attggaatac cgaccacgct aacaaagtgg
    12301 atagagacaa accccgccgc cgcgcactgt tctgcccagt aaccgatacg gccgatatga
    12361 tgcgagttat gtagcgccac ggcggcaatg ccgtgctgac gcgctttctc aatccccagc
    12421 gccatcgctt cgtgtgccgc gacctgacca aatgcgcgat cgccatcgag cgtgaccgcc
    12481 gcccccgctt ctttaacgac tttggcatga tggttaattt gcagatgccc ctgactccag
    12541 gagcgcacat agcttgggat catgccaata ccatgtgaat catgccctgc caggtttgcc
    12601 gcgattaaat gatcggcgac taatttcgct tcttgttcct cactacccat ctgacgaaat
    12661 acagcctgaa taaaactgtg cagcgtctga gcatcaaagc gatgaccact taccatggtt
    12721 aactcctgtt tatgttatgt gtttgttgtt tttttatgct gcacgccggg cgcgggaagg
    12781 tcaataaaga tgcaggaatt tcatattgcg gtggggtaaa agccggatga catggctcat
    12841 ccggctgaga taaaaattaa aatgcctgat taacgcgcgc caggccgtcg ctgatagttg
    12901 ccgcttcacc cgtagccatc aggcagcaag ccatctggat tttcagcgat tcgggaatcg
    12961 gttcgctgcc agcaaggcaa cgctcaatcc actgcgccgt ggtttccgga tcttttgctt
    13021 gtggcagtaa ctcgctacca gcagtgtcct gtttttcata cagtacccgc atcccttcac
    13081 ggtcaatgag attgatctgc gggcagcgct gcggattagc atacacttca ccttcagtac
    13141 catgcattag tagcgcccgc ccgccgatat cactaaagaa cttcgcgacg cgtccaatgt
    13201 attccggatg cgaaacgctg gaaagacgca gtgcctcacc ttcggcaaat ggcgtcgcca
    13261 gtttcgccag ggtatgtgca ctgttacgca cgcccatccg ccagcgcatc gccagttgtt
    13321 tttccagcgg cgggcaaaac gcgccgacag gcataaacac cggttgatgt tcgtcgagct
    13381 tcgcctgcgc ctgcccgccg tgcaacgttg gcgtaatacc catcagttcg aaaatggttt
    13441 ctgtcagcac gcgggttgga tcttcgctaa ccccgtgaac caccacggga aaaccgagtt
    13501 tgtgcaggag aatcgccaac aacggcgtca ggttggcctg tttacgcgcg ccgttgtaac
    13561 tggggatgac aatcggcatc ggcttgcctg caggcggcgt cagcttgatg gtgtgatttt
    13621 gcatggcttc gtaaaagccg agcatctctg cttccccttc ccctttgata cgtagcgcaa
    13681 tcaatacgcc gcccaactca aggtcaggga cttcaccatt gagcatatgg gcatacagac
    13741 cgcgcgcagt atcccggtct aaatcgcgcg cgtggttttt cccgcgccca atctctttaa
    13801 tgattttgcg atagtccatt tacgactcct tacctgactc acatcattaa cgccgccgac
    13861 ggcgtgggga tttcgttttt tctttctttt taactatacc ttcagggact tccggttgct
    13921 ctatcggaaa taccggtaat gcatccagta gtcgcttgcc ataatttttg gtcagcaagc
    13981 gtttgtcgta gataaccact tccccccagc aaccgtggct tcgaatcagt cgcccaacct
    14041 gctgaattag gttaaacgaa gcgctcggca ggctttgcac ctcaaacgga tagcggttga
    14101 ggcttttcag ccattcacct tcggtgatca ccaccggact gtcgatgggc ggaaaagcga
    14161 tcttgtggat atgcacctgg ctgagcagat cgcctttcag atcaagacct tcggcaaagg
    14221 actgtaagcc caccagcacg ctacgctcac cgttggcaac gcgtttgcgg tgcagttcaa
    14281 ctaagcgata acgtggctga tcgccctgca ccagcaacat caggcgtaaa tccgtcacat
    14341 agtcgagaaa gcgctgcatc gcacgcccgc tggcaaacag caccaacata ccgagatgtt
    14401 ttttgctctc cacctgttca cggaaaaagg ccgccatttc ggcaatgtgc tgctcttcgt
    14461 tgtcgatgga aggctcaacg cgcatccggg gaataacaat tttgccctgt tcacagtggt
    14521 taaaagggga atccagtgcc acaaaacggt cgcccgcttt ctctttcagc ccactcatct
    14581 cctgcaaacg cgaaaaactg ttcagcgaac gcaaggttgc ggaggtaaca ataatgtgcg
    14641 gaatactgcg ccacagcagc ctttccagct gatcgctgac acgtattccc acgcagtgaa
    14701 accaaaggtg tagctgccct tcgcgctctt cccgcgtcgc ccatttggtc accggcgcac
    14761 cggaagattg cgccagagac gccagccgcc agagtttgct ttgcgcctcg aacatcccca
    14821 atgcgcggtt catctgcaaa atcaaccgat gcagacgtac aatgtcatgg ctgccggttt
    14881 tctcacttaa atcgttaaga aataactccg ccaggccacg cagcatctcg gtgagttttg
    14941 ccagccgctg gcagatctcc agcacttcat ctggcagttc gcccatcgca aaacggtgct
    15001 ctgcctcctg cccggcaggc atgtagagat tgagaatgtt gtttaacgag gcgataagct
    15061 catacaactc ttcacaatgc gcattcaaac gttcagggat cgccagcggc gggatggtct
    15121 tcgggcgaaa ctgctccatg caggtagcga ccagtttcgt gaacaagtcc agctgtagcc
    15181 gataccacgg cgcggtgatt tcggcgctca tctccagcgc atcccgcgcc acatccggca
    15241 ggtgatgacc ttcgtccagc accagcagta aatttttcgg gtcaggcaat accgcttcgc
    15301 tttccatcgc cgccatcacc agcgcatggt ttgccaccac cacttccgct tcctgaattt
    15361 cccgacgagc gacaaaaaac ggacattcgc gatagtaata acagttgcgg ttaaggcaac
    15421 tggccttgtc ggtactcaaa cgacgccaga gatcatcatc tatagcgata tcagtatgat
    15481 cgcgcagacc atcccattta taagtgtcga gatcgccctt cagcttcgca caacgttttt
    15541 gctcttcctg attgttcggc gtcagttcgt cgtcaagaaa cgccagcaga tcctgttgcg
    15601 tgggttcagt gctggcgagc gctgtcagat tacgcgggca aacgtagcgc ccacgcccaa
    15661 aagcggcagt gaatttaaga tcgggaatga tctttttcag cagcggtaaa tctttgctgt
    15721 agatctgatc ctgcaatgcc acgttggcgg tacttaccac cagcgttttt tgctcttcgc
    15781 gggcaatggc gatgccgggg atcaaatagg agagcgtttt cccaacgccg gtaggggctt
    15841 caatcgccag atgccgccct tcttctccgg ccagcgtttt ggcgacgtct gcaatcatct
    15901 gccgctgcgg cgcacgggga ataaagtcgg ggatctgttc ctgaagcgcc ttataccagg
    15961 cggcaatttg cgctttaagc gcggcggtta atgccatgag aaaacctgaa atactgtata
    16021 aacagccaat attgtggcat ttttcggttt tcaggggtaa tggtttttct gtttctggaa
    16081 agcggctcgc aataaccagc aggatgatgc gtaaagcgct tcgcgctaaa ttcgaacaac
    16141 atgcagaact gcgtacactc ttgctggcaa ccgcgtccgc aaaactggtt gagcatacgc
    16201 aaaatgatgc ttactgggga gatggcggta atggtcaggg caagaatcgc ctggggtacc
    16261 ttttaatggc gttgcgcgga caattggctg cggagaagta acgcatcggg tggcttgtgg
    16321 gcgttaaaac agagaaatct ttttgccgcg ctcgcaaatg gctgacaagt gcttgttgtg
    16381 aaatgccgga tgcggcgcga gcgccttatc cggcctacaa aaacatgcac attcaatata
    16441 ttgcagagtt cacgtaggcc tgataagcgt agcgcatcag gcagttttgt gtttgtcatc
    16501 agtctcatgc cgggcatagc ccggcataaa agattactgc gcagcggcag gtttacgcgg
    16561 acggcggcga cgttgttgtt cgcctgccgg tttggcatcg ccgagacgac gagacggttt
    16621 ttctgcgggc ttcgcgttac cagacttcgc accactttcc gtacggcgtg gttgctgttg
    16681 accgcgacca ccgccctgtc cacgaccgcc gccacgttgc tggcgaccgt tctggatcgg
    16741 ttcggctttg attgacgggt ccggctcata ccccggaatc gcaatgcgcg ggatctcttt
    16801 tttcagcagt ttttcgatat cacgcagcag tttgtgttca tcaacacaca ccagcgacaa
    16861 tgcttcgcca gttgccgccg cacggccagt acgcccgata cggtgaacat aatcttccgg
    16921 tacgtttggt aactcatagt tgacgacgtg cggcaactct tcgatatcca gaccgcgcgc
    16981 agcgatgtca gttgccacca gtacacgaat atcgcccgat ttaaaatcgg ccagcgcacg
    17041 agtacgcgca ccttgcgatt tattaccgtg gatcgccgca ctacggatgc cgtctttatt
    17101 aagctgttca gccagatggt tagcgccatg tttggtacgg gtaaacacca gcacctgctg
    17161 ccagttccct ttcccaatca tatgcgacag caattcgcgt ttgcgtttct tatcgacaaa
    17221 gtgaacgtgc tgggtcacct gatcagacgc ggtattgcgg cgtgccactt cgatttccag
    17281 cgggttgtgc aacagttttt ccgccagggc tttaatatcg tcagagaagg tcgcggagaa
    17341 caacaggttc tggcgcttcg ctggcagttt tgttaacacg cgacggatat cgtggataaa
    17401 gcccatgtcg agcatgcggt cagcttcatc aaggacgagg atttcaacct gatccagctt
    17461 cactgcattt tgatgttcca ggtccagcag acgtcccggc gttgccacca gcacatcaac
    17521 gccgccgcgc aacttcatca tttgtgggtt aatgctgaca ccgccaaaca ccaccagcga
    17581 acgaatgttc agatatttgc tgtaatcacg gacgttttcg ccaatctgcg ccgccagttc
    17641 acgggtcggg gtaagaatga gcgcgcgtac cggacgacgc cctttggcgt gcggctggcg
    17701 agtgatcagg tgttgcaaca gcggcagcgt aaagcccgct gttttgccgg taccggtctg
    17761 ggcgctggcc atcaggtcgc ggccttccag caccgcaggg atcgcctgct gctgaatagg
    17821 ggtgggttca cggtaaccct gctcggcaac ggcgcgcagg atatcagggc ttaaacccaa
    17881 agaatcgaaa gacataacta ctccgaaccg ccccgaccgt taccggtgta gttttcaggg
    17941 agatactgaa aaggaaaatg acaaaaacca caatgtcatg agggagcgga gtgtagcagt
    18001 ttttgtgacg cagcgcataa attatccctg tgactggcgt agctaaaatt cttagccata
    18061 ctggacaact cccctatgga gtcataatct taatcaatca tttgattaag aatggactgt
    18121 gcgatgaata atcctgccat gacaatcaag ggtgaacagg cgaaaaaaca gctgattgct
    18181 gcggcactgg cacagtttgg tgaatatgga atgaacgcca ccacccgcga gatagccgcc
    18241 caggccgggc agaatatcgc tgccatcacc tactacttcg gttcgaaaga agatttgtat
    18301 ctcgcctgcg cccagtggat tgccgatttt attggcgagc agttccgtcc gcacgctgaa
    18361 gaagccgaac gcttgttcgc acaaccacag ccggatcggg ctgccatccg cgaactgatc
    18421 cttcgcgcct gcaagaacat gattaagctg ctcacccagg atgacaccgt caacctcagc
    18481 aaatttatct cccgtgagca gctctctccc acggcagcct atcacctggt gcacgaacag
    18541 gtaattagtc cgctacacag ccaccttaca cgcctgattg ccgcctggac cggctgcgac
    18601 gccaatgaca cccgcatgat ccttcatacc catgcgttga ttggtgagat tctggcattt
    18661 cgtcttggca aagaaaccat tttgttacgt accggctgga ccgcgttcga tgaagaaaag
    18721 accgaactga tcaaccagac ggtgacctgt catattgacc tgattttgca aggattatcg
    18781 caaaggagtt tgtagtgatg aaaaaaactg tcgtgatcgg actggcggta gtagtacttg
    18841 ccgccgtggt tgccggaggc tactggtggt atcaaagccg ccaggataac ggcctgactc
    18901 tgtatggcaa cgtggatatt cgtacggtga atcttagttt ccgtgttggg gggcgcgttg
    18961 agtcgctggc ggtggacgaa ggtgatgcga tcaaagcggg ccaggtgctg ggcgaactgg
    19021 atcacaagcc gtatgagatt gccctgatgc aggcgaaagc gggtgtttcg gtggctcagg
    19081 cgcagtatga cctgatgctt gccgggtatc gcgatgaaga aatcgctcag gccgccgcag
    19141 cggtgaaaca ggcgcaagct gcctatgact atgcgcagaa cttctataac cgccagcaag
    19201 gactgtggaa aagccgcacc atttcggcaa atgatctgga aaatgcccgc tcctcgcgcg
    19261 accaggcgca ggccacgctg aaatcagcgc aggataaatt gcgtcagtac cgttccggta
    19321 accgcgaaca agacatcgcc caggcgaaag ccagcctcga acaagcgcag gcgcaactgg
    19381 cgcaggcgga gctgaatttg caggattcga cgctggtcgc tccgtctgat ggcacgctgt
    19441 taacgcgcgc ggtagagcca ggcacggtgc ttaatgaagg tggcacggta tttaccgtct
    19501 cgctgacgcg tccggtgtgg gtacgcgcct atgttgatga acgtaatctt gaccaggccc
    19561 agccggggcg caaagtgctg ctttataccg atggtcgtcc gaacaaaccg tatcacgggc
    19621 agattggttt cgtttcgccg actgctgaat ttaccccgaa aaccgtcgaa acaccggatc
    19681 tgcgtaccga cctcgtctat cgtctgcgta ttgtggtgac cgacgccgat gatgcgttac
    19741 gccagggaat gccggtaacg gtgcaattcg gtgaagaggc aggacatgaa tgatgccgtt
    19801 atcacgctga acggcctgga aaaacgcttt ccgggcatgg acaagcccgc cgtcgcaccg
    19861 ctcgactgca ccattcacgc cggttatgtg acggggctgg ttgggccgga cggtgcgggc
    19921 aaaaccacgc tgatgcggat gctggcggga ttactgaaac ccgacagcgg cagtgccacg
    19981 gtgattggct ttgatccgat caaaaacgac ggcgcgctgc acgccgtgct cggctatatg
    20041 ccgcagaaat tcggcctgta tgaagatctg acggtgatgg agaacctcaa tctgtacgcg
    20101 gatttgcgca gcgtcaccgg cgaggcacgg aagcaaactt ttgctcgcct gctggagttt
    20161 acgtctcttg gcccgtttac cggacgcctg gcgggcaagc tctccggtgg gatgaaacaa
    20221 aaacttggcc tggcctgtac cctggtgggc gaaccgaaag tgttgctgct cgatgaaccc
    20281 ggcgtcggcg ttgaccctat ctcacggcgc gaactgtggc agatggtgca tgagctggcg
    20341 ggcgaaggga tgttaatcct ctggagtacc tcgtatctcg acgaagccga gcagtgccgt
    20401 gacgtgttac tgatgaacga aggcgagctg ctgtatcagg gagaaccgac ggccctgact
    20461 caaaccatgg ccggacgcag ctttctgatg accagcccgc acgagggcaa ccgcaaactg
    20521 ttgcaacggg cattgaaact gccgcaggtc agcgacggca tgattcaggg gaaatcggta
    20581 cgtctgatcc tcaaaaaaga ggctacacca gacgatattc gccatgccga cgggatgccg
    20641 gaaatcaaca ttaacgaaac tacgccgcgt tttgaagatg cgtttattga tttgctgggc
    20701 ggtgccggaa cctcggaatc gccgctgggc gcaatattac atacggtaga aggcacaccc
    20761 ggcgagacgg tgatcgaagc gaaggaacta accaagaaat ttggtgattt tgccgccacc
    20821 gatcacgtca actttgccgt taaacgcggg gagatttttg gtttactggg accaaacggc
    20881 gcgggtaaat cgaccacctt taagatgatg tgcggtttgc tggtaccgac ctctggtcag
    20941 gcgctggtgc tgggcatgga tctgaaagag agttccggta aagcgcgcca gcatctcggc
    21001 tatatggcgc aaaaattttc gctctacggc aacctgacgg tcgaacagaa tttacgcttt
    21061 ttctctggtg tgtatggctt acgcggtcgg gcgcagaacg aaaaaatctc ccgtatgagc
    21121 gaggcatttg gcctgaaaag tattgcctca cacgccaccg atgaactgcc attaggtttt
    21181 aaacagcggc tggcactggc ctgctcgctg atgcatgaac cggacattct gtttctcgac
    21241 gaaccgacgt ccggcgttga tcccctcacc cgccgcgaat tctggctgca catcaatagc
    21301 atggtagaga aaggcgtcac ggtgatggtt accacccact ttatggatga agcggaatat
    21361 tgcgaccgca tcggcctggt gtaccgcggg aaattaatcg ccagcggcac accggacgat
    21421 ttgaaagcgc agtcggccaa cgatgagcaa cccgatccca ctatggagca agcctttatt
    21481 cagttgatcc acgactggga taaggagcat agcaatgagt aatccgatcc tgtcctggcg
    21541 tcgcgtgcgg gcgctgtgcg tcaaagaaac gcggcagatc attcgcgatc cgagtagctg
    21601 gctgattgca gtagtgatcc cactgctact gctgtttatt tttggatacg gcattaacct
    21661 cgactccagc aagctgcgcg tcgggatttt actggaacag cgtagcgaag cggcgctgga
    21721 tttcacccac accatgaccg gttcgcccta catcgacgcc accatcagcg ataaccgtca
    21781 ggaactgatc gccaaaatgc aggcggggaa aattcgcggt ctggtggtta ttccggtgga
    21841 tttcgccgaa cagatggagc gcgccaacgc caccgcaccg attcaggtga tcaccgacgg
    21901 cagtgagccg aataccgcta actttgtgca ggggtatgtc gaagggatct ggcagatctg
    21961 gcaaatgcag cgcgcggagg ataacgggca gacttttgaa ccgcttattg atgtacaaac
    22021 ccgctactgg tttaacccgg cggcgattag ccagcacttt attatccccg gtgcggtgac
    22081 cattatcatg acggtcatcg gcgcgattct cacctcgctg gtggtggcgc gagaatggga
    22141 acgcggcacc atggaggctc tgctctctac ggagattacc cgcacggagc tgctgctgtg
    22201 taagctgatc ccttattact ttctcgggat gctggcgatg ttgctgtgta tgctggtgtc
    22261 ggtgtttatt ctcggcgtgc cgtatcgcgg gtcgctgctg attctgtttt ttatctccag
    22321 cctgttttta ctcagtaccc tggggatggg gctgctgatt tccacgatta cccgcaacca
    22381 gttcaacgcc gctcaggtgg ccctgaacgc ggcttttctg ccgtcgatta tgctttccgg
    22441 ctttatattt cagatagaca gtatgcccgc ggtgatccgc gcggtgacgt acattattcc
    22501 cgcacgttat ttcgtcagca ccctgcaaag cctgttcctc gccgggaata ttccagtggt
    22561 gctgatagtg aacgtgctgt ttttgatcgc ttcggcggtg atgtttatcg gcctgacgtg
    22621 gctgaaaacc aaacgtcggc tggattaggg agaagagcat gtttcatcgc ttatggacgt
    22681 taatccgcaa agagttgcag tcgctgctgc gcgaaccgca aacccgcgca attctgattt
    22741 tacccgtgct gattcaggtg atcctgttcc cgttcgccgc cacgctggaa gtgaccaacg
    22801 ccaccatcgc catctacgat gaagataacg gcgagcactc ggtagaactt actcaacgtt
    22861 ttgcccgcgc cagcgccttt acccatgtgc tgctgctgaa aagcccgcag gagatccgcc
    22921 cgaccatcga cacacaaaag gcgttactac tggtgcgttt cccagctgac ttctcgcgca
    22981 aactggatac cttccagacc gcgccgttgc agttgatcct cgacgggcgt aactccaaca
    23041 gtgcgcaaat tgccgccaac tacctgcaac agatcgtcaa aaattatcag caggagctgc
    23101 tggaaggaaa accgaaaccc aacaacagcg agctggtggt acgcaactgg tataacccga
    23161 atctcgacta caaatggttt gtggtgccgt cgctgatcgc catgatcacc actatcggcg
    23221 taatgatcgt cacttcactt tccgtcgccc gcgaacgtga acaaggtacg ctcgatcagt
    23281 tgctggtttc gcccctcacc acctggcaga tattcatcgg caaagccgta ccggcattaa
    23341 ttgtcgccac cttccaggcc accattgtgc tggcgattgg tatctgggcg tatcaaattc
    23401 ccttcgccgg atcgctggcg ctgttctact ttacgatggt gatttacggt ttatcgctgg
    23461 tgggattcgg tctgttgatt tcatcgctct gttcaacgca acagcaggca tttatcggcg
    23521 tgtttgtctt tatgatgccc gccattctcc tttccggtta cgtttcgccg gtggaaaaca
    23581 tgccaatgtg gctgcaaaat ctgacgtgga ttaaccctat tcgccacttt acggacatta
    23641 ccaagcagat ttatttgaag gatgcgagtc tggatattgt gtggaatagt ttgtggccgc
    23701 tactggtgat aacggccacg acagggtcag cggcgtacgc gatgtttaga cgtaaggtga
    23761 tgtaacttct tatctttcgc cagcaaagac accaccgccg ggccggcaag gattgccagc
    23821 cctgcaattg ccagcaaaaa gttgttttcc agtacgcggg acaacagcca cagaacaatc
    23881 agcgctgcgc caaaatacca ggtcgtggtc agctcttcca gcacgtctgc cacctcacgc
    23941 cagcgttgct cggggtggcg ctgaaacacc agcatcatca ctaccgtgac acaatacagt
    24001 gcgcataatc ctacgccgac gtgaaccaga gttttactca tccagcccac caccagcagc
    24061 gccactacca gtaaatgcaa cataatctgc cagcaactta ggccagttgc gacacgaaca
    24121 cgttgttgcc acttcatggc ttctctcctg aagaatttat ctctacttat tcagagtacc
    24181 gaactttacg gaaaattccg taagaccgca cctttttgtg acgccgacta cactatttca
    24241 atgagataag aggcaaagac aggaaaatta tgcccgatca aacacaacaa ttttcgttca
    24301 aggtgctcac catcaatatt cacaaaggct ttaccgcgtt taaccgacgc ttcattttgc
    24361 cggaacttcg cgacgccgtg cgtaccgtca gcgccgatat tgtttgcctg caggaagtga
    24421 tgggcgcgca cgaagttcat ccgctgcatg tggaaaactg gcccgatacc tcgcactacg
    24481 agtttctcgc cgacaccatg tggagcgatt tcgcctacgg tcgcaatgcc gtatacccgg
    24541 aaggacatca cggtaacgcc gttctttcac gttatcccat tgaacattat gagaatcgtg
    24601 atgtttcggt cgatggtgcg gaaaagcgcg gcgtgctcta ctgccgcatt gtgccgccga
    24661 tgaccgggaa agcgattcat gtgatgtgcg tacatctggg cctgcgtgag gcgcaccgtc
    24721 aggcgcagct tgcgatgctc gccgaatggg tgaatgagct accggacggc gaaccggtat
    24781 tggtagcggg tgatttcaac gactggcggc aaaaagctaa tcatccgtta aaagtgcagg
    24841 ccgggctgga tgagattttt acccgcgccc acggacgccc ggcgcgcacg tttccggtgc
    24901 aatttcctct actacgactg gacaggatct acgtcaaaaa tgccagcgcc agcgcgccaa
    24961 ccgcgttgcc gctgcggaca tggcgacacc tttctgatca tgccccttta agtgcggaga
    25021 ttcatttatg aaatgtagct ggcgcgaagg caataagatc cagttgctgg aaaacggcga
    25081 gcaatattat cccgcggtgt ttaaggcgat tggcgaggca caagaacgca tcattcttga
    25141 aacgtttatc tggtttgagg atgacgtcgg caaacagctg catgcggcgc tactggcagc
    25201 agcgcaacgc ggggtgaaag cggaagtctt gctggatggc tacggttcgc cggatctcag
    25261 cgatgagttt gtcaatgaac tgacggcagc tggcgtggtg ttccgctact acgatccccg
    25321 ccctcgcctt tttggtatgc gcaccaatgt gtttcgccgg atgcatcgca aaattgtggt
    25381 gatcgacgcg catatcgcct ttattggcgg gctgaattac tccgccgagc atatgtccag
    25441 ctacggcccg gaggccaaac aggattacgc ggttcgcctc gaagggccga ttgtcgaaga
    25501 tattctccag tttgagctgg aaaacctgcc aggacagagc gcggcgcgac gctggtggcg
    25561 acgtcatcat aaagcggaag agaaccgcca gccgggagaa gcgcaggtat tgctggtctg
    25621 gcgcgataac gaagaacatc gcgatgatat cgaacgtcac tatctgaaaa tgctcactca
    25681 ggcgcggcgg gaagtgatta tcgccaacgc ctacttcttc cccggctatc gttttttaca
    25741 cgccttgcgt aaagcggcac ggcgcggggt gcggatcaaa ctgatcattc agggcgaacc
    25801 ggatatgccg attgtcagag tcggcgcacg tttgctgtat aactatctgg ttaaaggcgg
    25861 cgttcaggtg tttgagtacc gccgccgccc gctccacggc aaagtggcat tgatggacga
    25921 tcactgggcg acagtagggt ccagtaatct cgatccgctc agtttgtcac tgaatctcga
    25981 agcaaatgtc atcatccacg atcgtaattt taaccagacg ctgcgcgata atctgaacgg
    26041 cattatcgcc gcagattgtc agcagatcga tgagaccatg ctgccgaaac gcacctggtg
    26101 gaacctgacc aaaagcgtgc tggcgttcca ctttctacgc cacttcccgg cgctggtggg
    26161 ctggcttccg gcgcacacgc cacgtctggc gcaggttgat ccccccgcac aaccgacaat
    26221 ggaaacgcag gatcgggtag aaactgaaaa cacgggggta aaaccctgat gagtaaatca
    26281 cacccgcgct ggcgcttagc aaagaagatc ctcacctggc tgttttttat cgcggtgatt
    26341 gtgttgctgg tggtctacgc caaaaaagtg gactgggaag aggtctggaa ggtcatccgc
    26401 gactacaatc gcgttgcgct gcttagtgcg gtcgggctgg tggtcgtcag ttatctgatt
    26461 tacggttgct acgacctgct cgcccgtttc tactgcggtc acaaactggc gaagcgccag
    26521 gtgatgctgg tgtcgtttat ctgctacgcc ttcaacctga cactcagtac ctgggtcggc
    26581 ggcattggta tgcgctatcg tttgtactcc cgactggggt taccgggcag cactattacg
    26641 cggatttttt cgctcagtat taccaccaac tggctaggct atattttgct agcgggggtt
    26701 atctttaccg caggcgtggt ggagttgccg gatcactggt atgtcgatca aactacgctg
    26761 cgtattctcg gtattggctt actgatgatt atcgcggttt atttgtggtt ttgcgctttc
    26821 gcgaagcacc gccatatgac catcaaaggg caaaagctgg tgctgccttc gtggaaattc
    26881 gccctcgccc agatgctgat ttccagcgtt aactggatgg taatgggagc gattatctgg
    26941 ctgttacttg gtcaaagcgt gaactatttc tttgtactgg gcgtgttgct ggttagtagt
    27001 attgctggcg tcatcgtgca tattccggcg ggaattggtg tgctggaagc ggtgtttatc
    27061 acgctactgg ctggggagca tacctccaaa ggcacaatta tcgccgccct gctcgcttac
    27121 cgtgtgctgt attactttat cccactgctg ctggcgctga tctgttacct gttgctggaa
    27181 agccaggcga agaagctacg ggcgaaaaat gaagcggcga tgtgaagtat agttaagttc
    27241 tttagtatgt gcatttacgg ctaatgaaaa aaccgcgtat gcctttgcca gacaagcgtt
    27301 atagctatag cggcaagaat cccaaagaag ccgataaaat agtaatagag tgttaatgca
    27361 cctacaaaag cacaacgaac cggactccat aattcttcac tatggctttt tcctgtcagt
    27421 tcaaggagct tatttcgtcc atgcgagata atgcctgacc ataagacaat cattaagcaa
    27481 cttattaccc aaatgggtcg ttcactcatt aatgtggcat tcacgatgat taccagggcc
    27541 aaccctgtca acgtcatcat aatgataaaa cggtgagaac caggatcaaa tttaaacaat
    27601 agacctgcca gcatactgat taaaaacatc gatcccgcag tgctgaacac gacgataatg
    27661 gcagatacag gcgctaaact cgcaacgaca atcgccacca aagagattaa aaacaaatat
    27721 attacgcccg cgatggcggt atttatcttg ttgatatctt tacttaagcc atacatagaa
    27781 agtaataata atcctatagc aacatacatt tgcggtgaat catattttgt tgccgtaatt
    27841 tcgagatact tagcgcccca ggccagtcct gcggttgtca ccaaacccaa agcggtaatg
    27901 agccatatgc cgtttaatat cctggcttcg tgctgaaaat ctaatttatt gctattttgc
    27961 gagtatgatt ccattctttt agttgtcctt agaaaaaaca ttaatattca ccatcaataa
    28021 atttaaaacc acgcatacaa aatacttttg catttatgga tacagaaaac gcttccgctc
    28081 gtccataagg catattttat acctggtact tgttaagtgt cgtttttaat aagaaaaaaa
    28141 tcccgacaca tggccgggat attgatgatg aaaaagaatt aacggcggtt gccgaagatc
    28201 cgcagcaaca tcaggaacag gttgatgaag tccagataca aggttaacgc gccaagaatg
    28261 gaatatttac gcaggttcga cgtgtcgcga gtatcaatct gctcacccat atttttcagt
    28321 ttctgggtgt catacgccgt caatccgaca aagacaatca cgccgatgta ggtgactgcc
    28381 cacatcaatg cttcgctttt cagccagaag ttaaccagcg atgccagcac aatgccgatt
    28441 aacgccataa acagcatatt gccaaagccg cttagatcgc gcttcgtggt gtaaccgtac
    28501 aggctcattg cgccgaacat cccggcagta acgacgaaag tactggcgat agaagcagcg
    28561 gtatagacaa tgaaaatact ggaaagcgta agacccgtca gcgccgaata aagcataaag
    28621 agcatcgtcg ttacacctgc gctcagcttt tgaatcatcg ctgataacac aataaccaat
    28681 gctaattgcg cgatgatcag accgattaaa aagacacggt tagtaaacaa cagctccatc
    28741 acggccgcgg aattagccgc ataccaggca acaaatgctg tcagcaacaa gccaacggtc
    28801 atccagccat agacttgcgc catataagtt tgcaagccag cccggggttg tacgattgaa
    28861 tcagaacgtg ggaatctgtc catgactttc tcctgaagat ataaggaata tcttaaggat
    28921 actgcaaaat gatgaggctg tgcatcgacg cagcgtaaac gtatgtactg agcggtgaaa
    28981 ttgccggacg cagcggtgcc ttatccggct aacaataaac taccagcgtt ttgccgcctg
    29041 ctgatcgctc tcccgagctt cgacccagcg gtcgccttcc ggcgtggctt cgcgcttcca
    29101 gaacggtgcg cgggttttga gataatccat aataaactgc ccggcttcaa acgcactgct
    29161 gcgatgcgca ctggtaacac cgacaaaaac aatttcatcg cccggccata attcaccaat
    29221 gcggtgaatt accgtgacgc gccccagcgg ccagcggtta cgcgcttcat caacgatttc
    29281 tgccagtgct ttttctgtca ttcccggata gtgttcgagg gttaatgctt tgacgctgtc
    29341 gcccaggtta tggttgcgca ccttaccggt aaaggtgact accgcaccgt cttcgtcacg
    29401 ctcagccagc cacgggtact cttctcctac gctgaacggc tgcgggccga caacaatttt
    29461 ggtttctgcc atcttaacct ccggttaccg gcgggaagaa agccacttcg tcgccgtcag
    29521 tcagcgaatg gtcaaaactc accagcgtct ggttgacagc tgccagcaat ttgccatctt
    29581 ccagcgccag cgcccagcga tcgctctgcg cagccagatg ctggcgtaac gcttcaacgg
    29641 ttgggaaatc cgcagccact tcggttgcat ctgttcccac caactcgcgc acctgggcga
    29701 aaaaaagaac gttaatcatc cgcttccacc ttaaagtcac ccgacttgcc gccgctcttc
    29761 gccagcaaac gtaccggacc aatcaccata tctttttgca ccgctttgca catgtcataa
    29821 atggtcagcg ccgccacgga ggccgcggtt aacgcttcca tttcgacacc ggttttcccg
    29881 gtcaggcggc ataaggtttc tatacgtacc cgattgtgct ccggctcggc ctgtaaattg
    29941 acttcaactt tgctgagcat cagcgggtga cacagcggga tcagatccca ggtgcgtttt
    30001 gccgcctgaa taccggcgat acgcgcagtg gcaaatacgt cgcctttgtg gtggcgacca
    30061 tcaataatca tcgccagcgt ctcgctgcgc atggtgacaa aggcttcggc gcgcgcttca
    30121 cgcacggttt ccgctttggc ggagacatcc accatgtgcg cttcgccagc ggcgttgata
    30181 tgggtcagtt gcgacatact tatttcttca aatgtggatg gaaattacac ggacgcgtac
    30241 gggcatccag ctgtggcgca atgatatttt cccatgcggt acggcacgct ttggtcgaac
    30301 ccggcatggc gaaaatcagc gttttgttag cgacgcccgc taccgcacga gattgcaacg
    30361 tggaagtgcc aatctcttcg aacgacaaca tccggaacac ttcaccaaaa ccttcaactt
    30421 cacggtcgaa caacggcagc agcgcttcgg gagcctgatc tccttccgtc aggccagtac
    30481 cacccgtaat caacaccact tgtacatcgt cgctggcgat ccacgcagat acctgagcgc
    30541 gaatagcgta gcggttttct ttcacaatgg ctttatcgac aacgtgatgg cccgcttctt
    30601 gcgccgagtc gcgcagatag tgaccggagg tatcgtcttc ttcaccgcga cgattagaaa
    30661 ccgtaagaat agcaatacgg gtcgggataa attcagtgct tacctgactc atctgatctc
    30721 tccttttgac gttttagccg ccaatgtacg ataagttttg cgtaataccg gtgttgtttt
    30781 gatgcaggaa atgggtctgt ttcttctccc gcagcgccgc tgaaatacgc gcttccagcg
    30841 cctgttgctg ggtatcgtct tccagcagat cgcgcaggtt aacgccgcct tcaccaaaca
    30901 ggcagagatg gagtttacca atggaggaaa cgcgcaggcg gttgcaagtg gcgcagaagt
    30961 ctttttcata cggcatgata aggccaatct ctccggcgta atccggatga caaaagactt
    31021 gcgcgggacc gtcgctgcgt tgacgtaatt ggtggatcca gccgcgacgc agtagctcgt
    31081 cacgcagaac ctgaccagag atgtgatgct tacggaagag ctcgctgccc tcgcccgttt
    31141 ccatcaattc gatgaaacgc agctggatag ggcgatgctg gatccagttc agaaaggtgt
    31201 cgagctgatg atgattaaca tcacgcatca gcacggtatt gactttgacc ttctcaaaac
    31261 cggcctcaaa tgcagcatcg atccctgcca tgacctggtt gaatttatcc tgcccggtaa
    31321 tggcatgaaa ctggcgagcg tcaaggctat caacgctgac gttgatgcca gtaagtccag
    31381 catcgcgcca gttcgccaca tcgcgttcca gacggtaacc attggtggtg accgcaatct
    31441 ggcggatagt gttgttttcc cgcacagcgg cgatgatatc ggtaaagtcg cggcgtaaag
    31501 atggctcacc gcccgtcaga cggacttttt cggtgccaag actggcgaag gcgcgcgtaa
    31561 cccggcgaat ttcatcgacg gtaagaaagc ctttattggt gacgccgctc ggtttgtagc
    31621 catccggcag gcagtaggtg caacgaaagt tacacacatc ggtaatcgac aggcgcaagt
    31681 agtaaaactt acgcgcaaat gcatcagtca gttgtgaagc catgtacacc tttccagata
    31741 cgggaggcga agtcatttct tccttcgccc tggtggcaat ctttacaagc attgtcacgg
    31801 ccaaagcacc gtatcagttg acccaggtgc agaggctaga gtgtttagtg gttaagccga
    31861 tactagcgtg taaatgagat ttttaccatc cacactttcg ctatgtaatc atgtatatag
    31921 cgtcatgatc gtgctatttc gctacagaat aagggaaaat gacggtttta ctttgatata
    31981 catcatcagg gaggcatagc gtgcatcgtc ctttagaggt agaagaaaat agtttgtatc
    32041 ctcaatattg gcaggttaat tgctgtttcc tcgcaatttg cgctactgta gcgcggataa
    32101 tctgactcca ggaaactata tgcgcaatcg tacgctggct gaccttgatc gtgtcgttgc
    32161 tctcggcgga gggcatggac tgggacgcgt tctctcatca ctttcgtctt tgggttctcg
    32221 tttaacgggt atcgtcacca ccaccgataa cggtggctcg acggggcgta ttcgccgctc
    32281 agaaggcggc attgcctggg gcgatatgcg caactgcctc aaccagctga taactgaacc
    32341 cagcgtcgct tccgcaatgt ttgaatatcg tttcggcggt aatggcgaac tttccgggca
    32401 caaccttgga aacctgatgt taaaggcact ggatcacctt agcgtgcgac ctcttgaagc
    32461 gatcaatctg attcgaaatc tgctgaaagt ggacgcacat ttgattccga tgtcggagca
    32521 tcctgttgat ctgatggcga ttgacgatca ggggcatgaa gtatacggcg aggtcaatat
    32581 cgaccagtta actgcgccga ttcaagagtt attgttaacg ccgaatgtac ccgcaacgcg
    32641 tgaggcggtt cacgctatca gtgaagcgga tctcatcatt attgggcctg gcagttttta
    32701 caccagcctg atgccaattc tgctgctgaa ggaaatcgca caggcattac gccgcacgcc
    32761 agcgccgatg gtctatatcg gcaatctggg gcgtgagttg agtttacctg cggctaatct
    32821 gaagctggaa agcaagctgg caattatgga gcagtatgtt ggtaaaaagg taattgatgc
    32881 ggtcatcgtc gggccaaaag aggatgtctc ggcggtgaaa gagcggattg tgattcagga
    32941 agtgctggag gccagcgata ttccgtatcg tcatgaccgc cagttgttgc atagcgcgct
    33001 ggaaaaggca ttacaggctt taggttaaca cgaagccgga agaatattcc ggcttcgtca
    33061 tcttgtcacg acgcggcaat aaacagctca cgcaactgat gcaactggtc acgaatttgc
    33121 gccgcttctt cgaactccag attctgcgcg tgttgcatca tcaacccttc cagttcatgg
    33181 atcttctgct gtaacgcttt aggcgacata tccatcggca cattatccgg ctcaacaatc
    33241 gggcgcgatt ttcctctgcc cttcgctttg gttttggcaa tgttctgccc cagcgccagg
    33301 atatcgacca ctttcttgtt caagccttgc ggcgtaatgc cgtgttcctc gttgtacttc
    33361 tgctgtttct cgcggcgacg ttcggtttcg ccaatcgctt tcgccattga tggggtgatc
    33421 ttatcgccgt agagaatcgc tttaccgtta acgttacgtg ccgcacgacc aatggtctgg
    33481 atcaacgaac gttcggaacg caggaagcct tctttgtcag cgtcgaggat cgccaccagc
    33541 gaaacttccg gcatatccag accttcgcgc agtaagttga tccctaccaa tacgtcgaac
    33601 tcacccagac gcaagtcgcg gataatctcc atacgttcga cggtgtcgat atctgagtga
    33661 agataacgca cgcgctcacc gtgttcttcg agatattcag tgagatcttc cgccatccgc
    33721 ttggtcagag ttgtaactag tacgcgttcg ttaattgccg ctcgctgacg aatctccgaa
    33781 agaagatcat ccacctgtgt tgccactggc cgcacttcga taatcgggtc gagtaagcct
    33841 gttggacgca ccacctgatc caccacatcg ccaccggatt tttccagctc gtaattaccc
    33901 ggcgtcgccg aaacatagat ggtttgcggc gctaatgctt cgaactcttc aaatttcagc
    33961 ggacggttat ccagcgctga tggcaggcgg aagccgtact ccaccagtgt ctctttacgc
    34021 gcccggtcac cgcgatacat gccgccaatt tgtggaatgg taacgtgaga ttcatcgacc
    34081 accagcagcc catcagcagg caggtaatca aacagcgtcg gcggtggctc acccggtcca
    34141 cgaccggaga ggaagcgcga gtagttctca atccccgaac agtagcctag ctcgttcatc
    34201 atctccagat caaactgggt acgctgggtc agccgctgat cttccagcag tttgttgttt
    34261 tccaatagca ctttacgtct ggcggccagc tcttctttga tctcctccat cgcctggacg
    34321 atgcgctcgc gtggcgtgac gtagtgcgtt ttcgggtaga tggtaaaacg tggaatagtg
    34381 gaaacaatct gcccggtcag cgggtcaaat aacgacaatc gttccacttc ctcgtcaaac
    34441 agctccacgc gaagtgcaat gtcatccgat tctgccggga agatatcaat cacctcgcca
    34501 cgaacacgga aagtaccacg ctggaaggct tgatcattac gagcatattg cagctccgcc
    34561 agtcggcgca ggatcgcgcg ctgatcgata atcataccga ccgtgagatg gagcatcatc
    34621 ttgagatata aatcaggatc gcccagacca taaatcgcgg aaacagacgc caccacaacc
    34681 acatcacgcc gctccagcat cgctttggtg gcggacaaac gcatctgttc gatatgttcg
    34741 tttaccgagg catctttctc aatgaaggtg tcggaactcg gtacataggc ctccggctga
    34801 tagtagtcgt agtaggagac gaaatattcc accgcgtttt ccgggaagaa ttctttcatt
    34861 tcgccataca gctgggccgc cagcgttttg ttgggcgcaa gtaccatggt tgggcgctga
    34921 aggtcagcaa tgacattggc aatggtgaag gttttcccgg agccagtcac gccaagtaac
    34981 gtctggtgcg ccaggccatc ttccagcccc tcttcgagac gtcgaatcgc ctccggctga
    35041 tcgccagaag gtttaaaagc ggaattcagt ttgaacggtt tactcatgag tcgctacctg
    35101 aaggagttgg gcgggcaggt atgtaatttt actcgtcgta cttaattatg ccaacaaatt
    35161 atactggata aaaaaacagt tcatcaccat aatatttctg ttacagcgta aactccgctg
    35221 tcaatcatga gcaaaattta ctctgcggcg agataaaact ccggccttac cgggttatcc
    35281 ccaaagcaac ggctttttta acatttgtca agatgattga tgacagtttt atggcaagag
    35341 atgcctgttc agtgactgcc attgcaatta tctaacctgt taaaaaataa aagatatttc
    35401 tttgagccgt ctttaacgcc aattcgcgtg caagccgcgt attctctcgc ttgcctcgtg
    35461 ttttctaact ctaatacaca tggttatcca caggaatagt ggataactgt ctgcagcccc
    35521 tatcccccgc cgcctgggca attcccacat tcaccgcatg atgcggaggt aaaatttttt
    35581 tcgtgatttt tttagaattt aattggttaa attgtagtca atcggagacg caatctcgct
    35641 cacaatttaa ccaagcacag gatgactaca ttaaggcaag attgatgtac tttccggttg
    35701 ccgcattttc tggattttct gcaagccagg gaatttcgcc tagcagcggc gcgggaatca
    35761 tgcgggtgag cgtggtcata tattcagcgt gacgttttcc cggaggcgtg acatcgttcg
    35821 ccacccaccc cgccagagtc agtccggcgt gttgtattgc ctgcgcagtc aacatcgcgt
    35881 gattaataca gccgagtttc acaccaacta ccagtatcac cggcagttgt tcctgtgtta
    35941 cccaatccgc aaaagtgaaa gtgtcagaaa gcggcgtaaa ccagccgcca gcaccttcca
    36001 ctaacaccca gtcagcctgt tgttcaagcg cgcgtaatcc ggcgctcatt accgatgatt
    36061 ctatcggcct gccctcttgc gcgctgacga tgtgcggcga ggtgggttct gcgaaggtgt
    36121 aaggatttac cgttgcgtaa tccagctgca ggctactgtt gcgctgtaac gccagcgcgt
    36181 cactattacg taagcctttc ggggtctttt cgctgccaga ggccaccggt ttataacctg
    36241 ccgtccggta gcctgctgcc tttgcggctt gtaaaagcgc gcagctggcg acagttttcc
    36301 ccacttcggt atccgttccg gtgacaaaat aacatttact cacgagcaat cactcccaaa
    36361 aaaagatgat acgtcagagg atatcgcccc tgttgttgcg gccaggccag ttgcaatcgc
    36421 tgcaactgcg aacgcgttaa tattcgcggg tcgcgccctt catgaagatg cgtggcaccg
    36481 atgcctttca gcgaacgcat ggcactgagc gcatcatcaa accacagcgt gatgggctga
    36541 atataatgtt gataatgcac ggcgtttagc gactgttcga ttttatctgg cggtaaaaag
    36601 cgattagcat gcgggcgctc gtccaccgcc tgccacgcct gatgcagttc gggtaacgat
    36661 ccctgcacca gcgtggtaaa cgcgaccacg cctccggggc gcaccacccg atacagctcg
    36721 cgaagtgccg tggataaatt accgcaccac tgcaccgcga gattgctcca tgcaagatcg
    36781 aacgttgcag tcgctaacgg cagggattcg atatctcccg ccagataatg gtctgcggca
    36841 tccttctggc gtgcctgaac aagcattggc ggagagagat ctaacgcagt cacctgcgcg
    36901 tgacgctctc gccagcggcg gctcatccag cctggtccac aacccgcatc cagtacgtgg
    36961 gtgtatttac gctgtgggag cattgccagt aaggcgtcag cactctggcg ctgtagatcg
    37021 gcgtgttgct catagtgtgc ggctgcccgt ccaaatgccg cagcaatggc ttgtttatta
    37081 accgttgcca tgcagcacct ccagcagacg gtcgatatcc tgcatttcat gcgctgcggt
    37141 tagcgttaag cgcagtcgcg cagtaccagc gggtacggtt ggtgggcgaa ttgccgtgac
    37201 ccaacagcct tgctggcgca gtttttctgc cagttgtaac gcacggctgt tatcaccgac
    37261 gatcaatggc tggatggcgc tccatgaatc agcaagcgta aacggcaaat cctgcactcc
    37321 ggcacgaaaa cgagtaatga gtgccgccag tttttcgcgc cgtgcatcac cctcatcact
    37381 gcgaatgacc gccagcgacg cacgtaatgc ctgcgcctga gcgggcggca tactggtgct
    37441 gtagataaga tggcgggcga attgcagcag ataatccgcc accgtattgg agcaaagcac
    37501 cgctgccccg ctgacgccaa atcctttgcc aaaagtcacc accagcaatt ctggttttac
    37561 cttttgcagc cagcagctgc cacgcccctg ctccccgata acgcccgtgc cgtgggcgtc
    37621 atcgaccatc aaccagccat cgtgctgttg cgttacctgc tggatttcct ccagtggcgc
    37681 actatcgccg tccatgctga acacaccttc tgtcactacc agttgctgcc ccggacaggg
    37741 ggaagcaagc agtcgcgcca gatgagtgac atcgttatga gcaaaacggc gaagcggcga
    37801 cgggcttaaa cttgcagcct ccagcaatga ggcatggcta agccggtcgg caacaatacg
    37861 gtcctctttc gccatcatcg cggcaatgac tgcctgatta gccgcaaaac cggagataaa
    37921 cagcagcgcc cgcgaatagc ccagccactc ggccagttct tcttccagca cctgatgcgc
    37981 cacgctataa ccgctgacgt gaccggaacc gccgctaccg acaccaaatt gttccgcccc
    38041 ctgctgccag gcgcggataa tttgcggatg atggcttaaa cctaaataat cgttactgga
    38101 aaagttcaga tactggcaat catctgccac cagccagcgt ccggctcctt gtgccaccgg
    38161 ataacggcga cgcaaggcat cagcaacacg ccgcgcatca agcgccgcgt cgattttctc
    38221 ctgccagatc atagtgctgc cgcgttgtaa tattcgtcag tgtccggggt catcagcgcc
    38281 tgctccagac gctgctgttg ttcgttatcg cccgccagca cggcagtttg ctgcggattt
    38341 agccccagtt tgcggaacag ttgcaggtct ttatcttctt ccggattcgg cgtggtcagc
    38401 agtttgcaac cgtagaaaat cgagtttgcg ccagccataa agcacatcgc ctgagtctgt
    38461 tcgttcatct gctcgcgtcc ggcagaaagg cgtacgtaag aggtcggcat catgatccgc
    38521 gctacggcaa tggtacggat aaaatcaaag gcatcgacat catcgttatc agcaagcggc
    38581 gtgcctttca ccttcaccag catgttgatt ggcacgcttt ccggcggcgt cggcaagttt
    38641 gtcagttgca gcaataatcc ggcgcgatct ttcaccgttt cgcctaagcc cacaatgcca
    38701 cctgaacaga ctttaatccc agcttctcgc actttttcca gcgtatcgag acgttcctga
    38761 taggtgcggg tggtgatgat attgccgtaa aactccggcg aggtgtcgag gttgtggtta
    38821 tagtaatcca gcccggcatt cgccaggcgc tgcgcttgag attcactcaa cgtgcccagc
    38881 gtcatacacg cctccagacc catcgctttt accccctgca ccatttgttc caggtagggc
    38941 atatcgcgtt cgtggggatt cttccacgcc gcgcccatac agaaacgcgt cgatcccgcc
    39001 gctttcgctt tgcgcgccga ctccagcacc tgttcaactt ccatcaaccg ctcggcttcc
    39061 agcccggttt tgtagcgcga gctttgcggg caatatttgc aatcttccgg acaagctccg
    39121 gtcttaatcg acagcaaagt gctgacctgc acctgacgag gatcgaaatg ctgacgatgc
    39181 acctgctgcg cttcaaacag cagatccagc aacggttttt caaataattc tgtgacttgc
    39241 gacaatgtcc agcgtaggcg gtgagccatg gggcttctcc aaaacgtgtt ttttgttgtt
    39301 aattcggtgt agactcgtaa acctaaatct tttcaatttg gtttacaagt cgattatgac
    39361 aacggacgat cttacctttg accaacgcca tatctggcac ccatacacat ccatgacctc
    39421 cccgctgccg gtttatccgg tggcaagcgc cgaaggttgc gagctaattt tgtctgacgg
    39481 cagacgcctg gttgacggta tgtcgtcctg gtgggcggcg atccacggtt acaatcaccc
    39541 gcagcttaat gcggcgatga agtcgcaaat tgatgctatg tcgcatgtga tgtttggcgg
    39601 tatcacccat gcgcccgcca ttgagctgtg ccgcaaattg gtagcaatga cgccgcaacc
    39661 gctggagtgc gtttttctcg cggactccgg ctccgtagcg gtagaagtgg cgatgaaaat
    39721 ggcgttgcag tactggcagg ccaaaggcga agcgcgccag cgttttctaa ccttccgcaa
    39781 tggttatcat ggcgatacct ttggcgcgat gtcggtgtgc gatccggaca actcaatgca
    39841 cagcctgtgg aaaggctatc tgccagaaaa cctgtttgct cccgccccgc aaagccgcat
    39901 ggatggcgaa tgggatgagc gcgatatggt gggctttgcc cgcctgatgg cagcgcatcg
    39961 tcatgaaatc gcggcggtga tcattgaacc gattgtccag ggcgcaggcg ggatgcgcat
    40021 gtaccatccg gaatggttaa aacgaatccg caaaatgtgc gatcgcgaag gtatcttgct
    40081 gattgccgac gagatcgcca ccggatttgg tcgtaccggc aaactgtttg cctgtgaaca
    40141 tgcagaaatc gcgccggata ttttgtgtct cggtaaagcc ttaaccggcg gcacaatgac
    40201 cctttccgcc acgctcacca cgcgcgaggt tgcggaaacc atcagtaacg gcgaagccgg
    40261 ctgctttatg catgggccaa cttttatggg caatccgctg gcctgcgcag cagcaaacgc
    40321 cagcctggcg attcttgaat ctggcgactg gcagcatcag gtggcggcta ttgaagcgca
    40381 gctacgcgag caacttgccc ccgcctgtga cgccgaaatg gttgccgatg tgcgcgtact
    40441 gggggcaatc ggcgtggtcg aaaccactcg tccggtgaat atggcagcgc tgcaaaaatt
    40501 ctttgtcgaa cagggtgtct ggatccggcc ttttggcaaa ctgatttacc tgatgccgcc
    40561 ctatattatt ctcccgcaac agttgcagcg tctgacggca gcggttaacc gtgcggtgca
    40621 ggatgaaaca tttttttgcc aataacggga agtcagcgtg agggtttctg gctacacttt
    40681 ctgcaaacta gaaaggaggg ttcatgaaac tcatcagtaa cgatctgcgc gatggcgata
    40741 agctgccgca tcgtcatgtc tttaacggca tgggttacga tggcgataat atttcaccgc
    40801 atctggcgtg ggatgatgtt cctgtgggaa cgaaaagttt tgttgtcacc tgctacgacc
    40861 cggatgcgcc aaccggctcc ggctggtggc actgggtagt tgttaattta cccgctgata
    40921 cccgcgtatt accgcaaggg tttggctctg gtctggtagc aatgccagac ggcgttttgc
    40981 agacgcgtac cgactttggt aaaaccggat acgatggcgc agcgccgccg aaaggcgaaa
    41041 cccatcgcta catttttacc gttcacgcgc tggatgtaga acgtattgat gtcgatgaag
    41101 gtgccagcgg cgcgatggtc gggtttaacg ttcatttcca ctctctggca agcgcctcga
    41161 ttactgcgat gtttagttaa tcactctgcc agatggcgca ataccatctg gtatcactta
    41221 aaggtattaa aaacaacttt ttgtcttttt accttcccgt ttcgctaaag ttagtataaa
    41281 aaagttgaat
//
LOCUS       DADSYM010000018        40851 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2415
            SAMN08039579-rid11723173.denovo.018, whole genome shotgun sequence.
ACCESSION   DADSYM010000018 DADSYM010000000
VERSION     DADSYM010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039579
            Sequence Read Archive: SRR6362533
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 40851)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40851)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980162.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 03:39:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,484
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 4,347
            CDSs (with protein)               :: 4,347
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 43 of 85
            Pseudo Genes (incomplete)         :: 37 of 85
            Pseudo Genes (internal stop)      :: 19 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40851
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039579-rid11723173.denovo.018"
                     /strain="LGE2415"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8302 N 95.9628 W"
                     /collection_date="2015-08-24"
     gene            70..894
                     /gene="rhaD"
                     /locus_tag="J1Y83_000832"
     CDS             70..894
                     /gene="rhaD"
                     /locus_tag="J1Y83_000832"
                     /EC_number="4.1.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulose-1-phosphate aldolase"
                     /protein_id="HBA7629009.1"
                     /translation="MQNITQSWFVQGMIKATTDAWLKGWDERNGGNLTLRLDDADIAP
                     YHDNFHQQPRYIPLSQPMPLLANTPFIVTGSGKFFRNVQLDPAANLGIVKVDSDGAGY
                     HILWGLTNEAVPTSELPAHFLSHCERIKATNGKDRVIMHCHATNLIALTYVLENDTAV
                     FTRQLWEGSTECLVVFPDGVGILPWMVPGTDEIGQATAQEMQKHSLVLWPFHGVFGSG
                     PTLDETFGLIDTAEKSAQVLVKVYSMGGMKQTISREELIALGQRFGVTPLASALAL"
     gene            904..1218
                     /gene="rhaM"
                     /locus_tag="J1Y83_000833"
     CDS             904..1218
                     /gene="rhaM"
                     /locus_tag="J1Y83_000833"
                     /EC_number="5.1.3.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose mutarotase"
                     /protein_id="HBA7629010.1"
                     /translation="MIRKAFVMQVNPDAHEEYQRRHNPIWPELEAVLKSHGAHNYAIY
                     LDKARNLLFATIEIESEERWNAVASTDVCQRWWKYMTDVMPANADNSPVSSELQEVFY
                     LP"
     gene            complement(1259..2653)
                     /locus_tag="J1Y83_000834"
     CDS             complement(1259..2653)
                     /locus_tag="J1Y83_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HBA7629011.1"
                     /translation="MKKSTLSLAIGLLLACSTGMAKTQHLTLEQRMALLEERLEAAEM
                     RAAKAEGQVKQLQTQQAAEIREIKTAQGNTPVNGQSTTESEKKNATPPNLLLSGYGDL
                     KIYGDVEFNMDAESNHGLLAMTNADVNSDPTNEQWNLNGRILLGFDGMRKLDNGYFAG
                     FSAQPLGDMHGSVNIDDAVFFFGKENDWKVKVGRFEAYDMFPLNQDTFVEHSGNTAND
                     LYDDGSGYIYMMKEGRGRSNAGGNFLVSKQLDNWYFELNTLLEDGTSLYNDGNYHGRD
                     MEQQKNVAYLRPVIAWSPTEEFTVSAAMEANVVNNAYGYTDSKGNFVDQSDRTGYGMS
                     MTWNGLKTDPENGIVVNLNTAYLDANNEKDFTAGINALWKRFELGYIYAYNKIDEFSG
                     VVCDNDCWIDDEGTYNIHTIHASYQFANVMDMENFNIYLGTYYSILDSDGDKIHGDDS
                     DDRYGARVRFKYFF"
     gene            complement(3007..3568)
                     /locus_tag="J1Y83_000835"
                     /pseudo
     CDS             complement(3007..3568)
                     /locus_tag="J1Y83_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000828060.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YiiG family protein"
     gene            complement(3650..4483)
                     /gene="fdhD"
                     /locus_tag="J1Y83_000836"
     CDS             complement(3650..4483)
                     /gene="fdhD"
                     /locus_tag="J1Y83_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HBA7629012.1"
                     /translation="MKKTQQKEIENVTNITGVRQIELWRRDDLQHPRLDEVAEEVPVA
                     LVYNGISHVVMMASPKDLEYFALGFSLSEGIIESPRDIFGMDVVPSCNGLEVQIELSS
                     RRFMGLKERRRALAGRTGCGVCGVEQLNDIGKPVQPLPFTQTFDLNKLDDALRHLNDF
                     QPVGQLTGCTHAAAWMLPSGELVGGHEDVGRHVALDKLLGRRSQEGESWQQGAVLVSS
                     RASYEMVQKSAMCGVEILFAVSAATTLAVEVAERCNLTLVGFCKPGRATVYTHPQRLS
                     N"
     gene            4677..7727
                     /gene="fdnG"
                     /locus_tag="J1Y83_000837"
     CDS             4677..7727
                     /gene="fdnG"
                     /locus_tag="J1Y83_000837"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:5262..5264,aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit alpha"
                     /protein_id="HBA7629013.1"
                     /translation="MQVSRRQFFKICAGGMAGTTAAALGFAPSVALAETRQYKLLRTR
                     ETRNTCTYCSVGCGLLMYSLGDGAKNAKASIFHIEGDPDHPVNRGALCPKGAGLVDFI
                     HSESRLKFPEYRAPGSDKWQQISWEEAFDRIAKLMKEDRDANYIAQNAEGVTVNRWLS
                     TGMLCASASSNETGYLTQKFSRALGMLAVDNQARVUHGPTVASLAPTFGRGAMTNHWV
                     DIKNANLVVVMGGNAAEAHPVGFRWAMEAKIHNGAKLIVIDPRFTRTAAVADYYAPIR
                     SGTDIAFLSGVLLYLLNNEKFNREYTEAYTNASLIVREDYGFEDGLFTGYDAEKRKYD
                     KSTWTYELDENGFAKRDTTLQHPRCVWNLLKQHVSRYTPDVVENICGTPKDAFLKVCE
                     YIAETSAHDKTASFLYALGWTQHSIGAQNIRTMAMIQLLLGNMGMAGGGVNALRGHSN
                     IQGLTDLGLLSQSLPGYMTLPSEKQTDLQTYLTANTPKPLLEGQVNYWGNYPKFFVSM
                     MKAFFGDKATAENSWGFDWLPKWDKGYDVLQYFEMMKEGKVNGYICQGFNPVASFPNK
                     NKVIGCLSKLKFLVTIDPLNTETSNFWQNHGELNEVDSSKIQTEVFRLPSTCFAEENG
                     SIVNSGRWLQWHWKGADAPGIALTDGEILSGIFLRLRKMYAEQGGANPDQVLNMTWNY
                     AIPHEPSSEEVAMESNGKALADITDPATGAVIVKKGQQLSSFAQLRDDGTTSCGCWIF
                     AGSWTPEGNQMARRDNADPSGLGNTLGWAWAWPLNRRILYNRASADPQGNPWDPKRQL
                     LKWDGTKWTGWDIPDYSAAPPGSGVGPFIMQQEGMGRLFALDKMAEGPFPEHYEPFET
                     PLGTNPLHPNVISNPAARIFKDDAEALGKADKFPYVGTTYRLTEHFHYWTKHALLNAI
                     LQPEQFVEIGESLANKLGIAQGDTVKVSSNRGYIKAKAVVTKRIRTLKANGKDIDTIG
                     IPIHWGYEGVAKKGFIANTLTPFVGDANTQTPEFKSFLVNVEKV"
     gene            7740..8642
                     /gene="fdxH"
                     /locus_tag="J1Y83_000838"
     CDS             7740..8642
                     /gene="fdxH"
                     /locus_tag="J1Y83_000838"
                     /EC_number="1.17.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase subunit beta"
                     /protein_id="HBA7629014.1"
                     /translation="MAYQSQDIIRRSATNGLTPAPQARDFQEEVAKLIDVTTCIGCKA
                     CQVACSEWNDIRDTVGNNIGVYDNPNDLSAKSWTVMRFSEVEQNDKLEWLIRKDGCMH
                     CSDPGCLKACPAEGAIIQYANGIVDFQSEQCIGCGYCIAGCPFDIPRLNPEDNRVYKC
                     TLCVDRVVVGQEPACVKTCPTGAIHFGTKESMKTLASERVAELKTRGYDNAGLYDPAG
                     VGGTHVMYVLHHADKPNLYHGLPENPEISETVKFWKGIWKPLAAVGFAATFAASIFHY
                     VGVGPNRADEEENNLHEEKDEERK"
     gene            8639..9274
                     /gene="fdoI"
                     /locus_tag="J1Y83_000839"
     CDS             8639..9274
                     /gene="fdoI"
                     /locus_tag="J1Y83_000839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase cytochrome b556 subunit"
                     /protein_id="HBA7629015.1"
                     /translation="MKRRDTIVRYTAPERINHWITAFCFILAAVSGLGFLFPSFNWLM
                     QIMGTPQLARILHPFVGVVMFASFIIMFFRYWHHNLINRDDIFWAKNIRKIVVNEEVG
                     DTGRYNFGQKCVFWAAIIFLVLLLVSGVIIWRPYFAPAFSIPVIRFALMLHSFAAVAL
                     IVVIMVHIYAALWVKGTITAMVEGWVTSAWAKKHHPRWYREVRKTTEKKAE"
     gene            9271..10200
                     /gene="fdhE"
                     /locus_tag="J1Y83_000840"
     CDS             9271..10200
                     /gene="fdhE"
                     /locus_tag="J1Y83_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory protein FdhE"
                     /protein_id="HBA7629016.1"
                     /translation="MSIRIIPQDELGSSEKRTADMIPPLLFPRLKNLYNRRAERLREL
                     AENNPLGDYLRFAALIAHAQEVVLYDHPLEMDLTTRIKEASAQGKPPLDIHVLPRDKH
                     WQKLLMALIAELKPEMSGPALAVIENLEKASTQELEDMASALFASDFSSVSSDKAPFI
                     WAALSLYWAQMANLIPGKARAEYGEQRQYCPVCGSMPVSSMVQIGTTQGLRYLHCNLC
                     ETEWHVVRVKCSNCEQSGKLHYWSLDDEQAAIKAESCDDCGTYLKILYQEKEPKVEAV
                     ADDLASLVLDARMEQEGYARSSINPFLFPGEGE"
     gene            complement(10422..10640)
                     /locus_tag="J1Y83_000841"
     CDS             complement(10422..10640)
                     /locus_tag="J1Y83_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312842.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HBA7629017.1"
                     /translation="MAMNTVFLHLSEEAIKRLNKLRGWRKVSRSAILREAVEQYLERQ
                     QFPVRKAKGGRQRDEAVGVEELCKQHKE"
     gene            complement(10698..10880)
                     /locus_tag="J1Y83_000842"
     CDS             complement(10698..10880)
                     /locus_tag="J1Y83_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000236453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA7629018.1"
                     /translation="MVINSAIPFSSAIGELILLYECKALYARFCWMETGKFTGVKNPE
                     SVSQKQNNMKKSAFVN"
     gene            11039..11329
                     /locus_tag="J1Y83_000843"
     CDS             11039..11329
                     /locus_tag="J1Y83_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytoplasmic protein"
                     /protein_id="HBA7629019.1"
                     /translation="MHVISHKPFNEAIKRFPPYEGALIDLLNLLEKTEFSDPGEMKRY
                     IPSLDNFKYRDKWWVINIAGNHLRLIAYIDFRLQKCFIKHTVIYNEYDRFTT"
     gene            11346..11801
                     /locus_tag="J1Y83_000844"
     CDS             11346..11801
                     /locus_tag="J1Y83_000844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001534936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBA7629020.1"
                     /translation="MRTHHTPAEDSANFGMLVKTFTHAVKDIPLCGPKADQEEYHNAL
                     KLIEFLVDRDDLENPLFELLCVRIREYENNAPEFQHFNQRLASTPCGVSLLRILMDQH
                     GLKAADLAQELGSKSNVSNILNGRRALTVRHIKALSERFNLPAEAFIDK"
     gene            complement(11812..12753)
                     /gene="fabY"
                     /locus_tag="J1Y83_000845"
                     /gene_synonym="yiiD"
     CDS             complement(11812..12753)
                     /gene="fabY"
                     /locus_tag="J1Y83_000845"
                     /gene_synonym="yiiD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001666856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid biosynthesis protein FabY"
                     /protein_id="HBA7629021.1"
                     /translation="MYHLRVPQTEEELERYYQFRWEMLRKPLHQPKGSERDAWDAMAH
                     HQMVVDEQGNLVAVGRLYINADNEASIRFMAVHPDVQDKGLGTLMAMTLESVARQEGV
                     KRVTCSAREDAAEFFAKLGFVNQGEITTPTTTPIRHFLMIKPVATLDDILHRGDWCAQ
                     LQQAWYEHIPLSEKMGVRIQQYTGQKFITTMPETGNQNPHHTLFAGSLFSLATLTGWG
                     LIWLMLRERHLGGTIILADAHIRYSKPISGKPHAVADLGALSGDLDRLARGRKARVQM
                     QVEIFGDETPGAVFEGTYIVLPAKPFGPYEEGGNEEE"
     gene            complement(12798..13235)
                     /gene="dtd"
                     /locus_tag="J1Y83_000846"
     CDS             complement(12798..13235)
                     /gene="dtd"
                     /locus_tag="J1Y83_000846"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000560980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-tyrosyl-tRNA(Tyr) deacylase"
                     /protein_id="HBA7629022.1"
                     /translation="MIALIQRVTRASVTVEGEVTGEIGAGLLVLLGVEKDDDEQKANR
                     LCERVLGYRIFSDAEGKMNLNVQQAGGSVLVVSQFTLAADTERGMRPSFSKGASPDRA
                     EALYDYFVERCRQQEMNTQTGRFAADMQVSLVNDGPVTFWLQV"
     gene            complement(13232..14104)
                     /locus_tag="J1Y83_000847"
     CDS             complement(13232..14104)
                     /locus_tag="J1Y83_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor BrkB family protein"
                     /protein_id="HBA7629023.1"
                     /translation="MLKTIQDKARHRTRPLWAWLKLLWQRIDEDNMTTLAGNLAYVSL
                     LSLVPLVAVVFALFAAFPMFSDVSIQLRHFIFANFLPATGDVIQRYIEQFVANSNKMT
                     AVGACGLIVTALLLMYSIDSALNTIWRSKRARPKIYSFAVYWMILTLGPLLAGASLAI
                     SSYLLSLRWASDLNTVIDNVLRIFPLLLSWISFWLLYSIVPTIRVPNRDAIVGAFVAA
                     LLFEAGKKGFALYITMFPSYQLIYGVLAVIPILFVWVYWTWCIVLLGAEITVTLGEYR
                     KLKQAAEQEEDDEP"
     gene            complement(14098..14697)
                     /gene="yihX"
                     /locus_tag="J1Y83_000848"
     CDS             complement(14098..14697)
                     /gene="yihX"
                     /locus_tag="J1Y83_000848"
                     /EC_number="3.1.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312835.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphatase"
                     /protein_id="HBA7629024.1"
                     /translation="MLYIFDLGNVIVDIDFNRVLGAWSDLTRVPLATLKKSFHMGEAF
                     HQHERGEISDEAFAEALCHEMALPLSYEQFSHGWQAVFVALRPEVIAIMHKLREQGHR
                     VVVLSNTNRLHTTFWPEEYPEIRDAADHIYLSQDLGMRKPEARIYQHVLQAEGFSPDD
                     TVFFDDNADNIEGANRLGITSILVKDKTTIPDYFAKVLC"
     gene            complement(14796..15581)
                     /locus_tag="J1Y83_000849"
     CDS             complement(14796..15581)
                     /locus_tag="J1Y83_000849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418320.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HBA7629025.1"
                     /translation="MSLTELTGNPRHDQLLMLIAERGYMNIDELANLLDVSTQTVRRD
                     IRKLSEQGLITRHHGGAGRASSVVNTAFEQREVSQTEEKKAIAEAVADYIPDGSTIFI
                     TIGTTVEHVARALLNHNHLRIITNSLRVAHILYHNPRFEVMVPGGTLRSHNSGIIGPS
                     AASFVADFRADYLVTSVGAIESDGALMEFDVNEANVVKTMMAHARNILLVADHTKYHA
                     SAAVEIGNVAQVTALFTDELPPAALKSRLQDSQIEIILPQEDA"
     gene            complement(15615..16511)
                     /gene="yihV"
                     /locus_tag="J1Y83_000850"
     CDS             complement(15615..16511)
                     /gene="yihV"
                     /locus_tag="J1Y83_000850"
                     /EC_number="2.7.1.184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418319.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfofructose kinase"
                     /protein_id="HBA7629026.1"
                     /translation="MIRVACVGITVMDRIYYVEGLPTESGKYVAKNYTEVGGGPAATA
                     AVAAARLGAQVDFIGRVGDDDTGNSLLAELESWGVNTRYTKRYNQAKSSQSAIMVDAK
                     GERIIINYPSPDLLPDAEWLEEIDFSQWDVVLADVRWHDGAKKAFTLARQAGVMTVLD
                     GDITPQDISELVALSDHAAFSEPGLARLTGVKEMASALKQAQTLTNGHVYVTQGSAGC
                     DWLENGGRQHQPAFKVDVVDTTGAGDVFHGALAVALATGGDLAESVRFASGVAALKCT
                     RPGGRAGIPDCDQTRSFLSLFV"
     gene            16679..17575
                     /gene="yihU"
                     /locus_tag="J1Y83_000851"
     CDS             16679..17575
                     /gene="yihU"
                     /locus_tag="J1Y83_000851"
                     /EC_number="1.1.1.373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfolactaldehyde 3-reductase"
                     /protein_id="HBA7629027.1"
                     /translation="MAAIAFIGLGQMGSPMASNLLQQGHQLCVFDVNTEAVQHLVDKG
                     ATPAANPAQAAKGAEFIITMLPNGDLVRHVLFGENGVCESLSSDALVIDMSTIHPLQT
                     DKLIADMQARGFSMMDVPVGRTSANAITGTLLLLAGGTAEQVERATPILMAMGSELIN
                     AGGPGMGIRVKLINNYMSIALNALSAEAAVLCEALNLPFDVAVKVMSGTAAGKGHFTT
                     SWPNKVLSGDLSPAFMIDLAHKDLGIALDVANQLHVPMPLGAASREVYSQARAAGRGR
                     QDWSAILEQVRVSAGMTAKVKM"
     gene            17599..18477
                     /gene="yihT"
                     /locus_tag="J1Y83_000852"
     CDS             17599..18477
                     /gene="yihT"
                     /locus_tag="J1Y83_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfofructosephosphate aldolase"
                     /protein_id="HBA7629028.1"
                     /translation="MNKYTINDITRASGGFAMLAVDQREAMRMMFAAAGAPAPVANSV
                     LTDFKVNAAKTLSPYASAILVDQQFCYRQVVEQNAIAKSCAMIVAADEFIPGNGIPVD
                     SVVIDRKINPLQIKQDGGKALKLLVLWRSDEDAQQRLDMVKEFNEMCHSHGLVSIIEP
                     VVRPPRRGDKFDREQAIIDAAKELGDSGADLYKVEMPLYGKGPQQELLSASQRLNDHI
                     NMPWVILSSGVDEKLFPRAVRVAMTAGASGFLAGRAVWASVVGLPDNELMLRDVCAPK
                     LQQLGDIVDEMMAKRR"
     gene            18493..19734
                     /gene="yihS"
                     /locus_tag="J1Y83_000853"
     CDS             18493..19734
                     /gene="yihS"
                     /locus_tag="J1Y83_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfoquinovose isomerase"
                     /protein_id="HBA7629029.1"
                     /translation="MKWFNTLSHNRWLEQETDRIFDFGKKSVVPTGFGWLGNKGQIKE
                     EMGTHLWITARMLHVYSVAAAMGRPGAYSLVDHGIKAMNGALRDKKYGGWYACVNDEG
                     VVDASKQGYQHFFALLGAASAVTTGHPEARKLLDYTIEIIEKYFWSEEEQMCLESWDE
                     AFSKTEEYRGGNANMHAVEAFLIVYDVTHDKKWLDRAIRVASVIIHDVARNNHYRVNE
                     HFDTQWNPLPDYNKDNPAHRFRAFGGTPGHWIEWGRLMLHIHAALEARCEQPPAWLLE
                     DAKGLFNATVRDAWAPDGADGIVYTVDWEGKPVVRERVRWPIVEAMGTAYALYTVTGD
                     RQYETWYQTWWDYCIKYLMDYENGSWWQELDADNKVTTKVWDGKQDIYHLLHCLVIPR
                     IPLAPGLAPAVAAGLLDINAK"
     gene            19801..20703
                     /locus_tag="J1Y83_000854"
     CDS             19801..20703
                     /locus_tag="J1Y83_000854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001417111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose-1-epimerase"
                     /protein_id="HBA7629030.1"
                     /translation="MQITNMHCSGQTVSLAAGDYHATIVTVGAGLAELTFQGCHLVIP
                     HKPEEMPLAHLGKVLIPWPNRIANGCYRYQGQEYQLPINEHGSKAAIHGLLAWRDWQI
                     SELSATSVTLTAFLPPSYGYPFMLASQVVYSLNARTGLSVEIASQNIGTVAAPYGVGI
                     HPYLTCNLTSVDEYLFQLPANQVYAIDEHANPTTLHHVDELDLNFTQAKKIAATKIDH
                     TFKTANDLWEMTITHPQQALSVSLCSDQPWVQVYSGEKLQRQGLAVEPMSCPPNAFNS
                     GIDLLLLEPGKTHRLFFNIHGQHN"
     gene            20764..21639
                     /locus_tag="J1Y83_000855"
     CDS             20764..21639
                     /locus_tag="J1Y83_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HBA7629031.1"
                     /translation="MDFFTLFSPDGVMLETNVVCIVIALMFLYTFVGICAGFGGGLTT
                     MPLVTLMLPVKMATPLSVIVGTATALYATWLSRKETDWRSAMVLIIFSFLGIPVGLYA
                     LSYLPDHFMKVGLGGFLILYSFYSMFIPRLPIYDRSWIAAPIGAVAGALGAAFSTNGP
                     PMVIYGMLRNLGPAAFRGTLNAFFTANNIAVIGGLATSGILTVSIFKLVLFCIPTMIL
                     GSLVGQYVHKRISVKVFRVMVFLLLIASGSMLIKGALGIPAAAALLPPFTLLVILQLA
                     FGKKVAAIRRAEESK"
     gene            21745..23781
                     /gene="yihQ"
                     /locus_tag="J1Y83_000856"
     CDS             21745..23781
                     /gene="yihQ"
                     /locus_tag="J1Y83_000856"
                     /EC_number="3.2.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfoquinovosidase"
                     /protein_id="HBA7629032.1"
                     /translation="MDTPRPQLLDFQFHQNNDSFTLRFQDRLILIHSKDNPCLWIGSG
                     IADIDMFRGNFSIKDKLQEKIALTDAIVSQSPDGWLIHFSRGSDISATLNISADDHGR
                     LLLELQNDNLNHNRIWLRLAAQPEDHIYGCGEQFSYFDLRGKPFPLWTSEQGVGRNKQ
                     TYVTWQADCKENAGGDYYWTFFPQPTFVSTQKYYCHVDNSCYMNFDFSAPEYHELALW
                     EDKATLRFECADTYISLLEKLTALLGRQPELPDWIYDGVTLGIQGGTEVCQKKLDTMR
                     NAGVKVNGIWAQDWSGIRMTSFGKRVMWNWKWNSENYPQLDSRIKQWNKEGVQFLAYI
                     NPYVASDKDLCEEAAKRGYLAKDAAGGDYLVEFGEFYGGVVDLTNPEAYAWFKEVIKK
                     NMIELGCGGWMADFGEYLPTDTYLHNGVSAEIMHNAWPALWAKCNYEALEETGKLGEI
                     LFFMRAGSTGSQKYSTMMWAGDQNVDWSLDDGLASVVPAALSLAMTGHGLHHSDIGGY
                     TTLFEMKRSKELLLRWCDFSAFTPMMRTHEGNRPGDNWQFDSDAETIAHFARMTTVFT
                     TLKPYLKEAVALNAKSGLPVMRPLFLHYEDDAQTYTLKYQYLLGRDILVAPVHEEGRS
                     DWTLYLPEDNWVHAWTGEAFRGGEVTVNAPIGKPPVFYRADSEWAALFASLKSI"
     gene            23827..25212
                     /locus_tag="J1Y83_000857"
     CDS             23827..25212
                     /locus_tag="J1Y83_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBA7629033.1"
                     /translation="MSHITTEDPATLRLPFKEKLSYGIGDLASNILLDIGTLYLLKFY
                     TDVLGLPGTYGGIIFLISKFFTAFTDMGTGIMLDSRRKIGPKGKFRPFILYASFPVTL
                     LAIANFVGTPFDVTGKTVMATILFMLYGLFFSMMNCSYGAMVPAITKNPNERASLAAW
                     RQGGATLGLLLCTVGFVPVMNLIEGNQQLGYIFAATLFSLFGLLFMWICYSGVKERYV
                     ETQPTNPAQKPGLLQSFRAIAGNRPLFILCIANLCTLGAFNVKLAIQVYYTQYVLNDP
                     ILLSYMGFFSMGCIFIGVFLMPGAVRRFGKKKVYIGGLLIWVLGDLLNYFFGGGSVSF
                     VAFSCLAFFGSAFVNSLNWALVSDTVEYGEWRTGVRSEGTVYTGFTFFRKVSQALAGF
                     FPGWMLTQIGYVPNVAQADHTIEGLRQLIFIYPSALAVVTIVAMGCFYSLNEKMYVRI
                     VEEIEARKRTA"
     gene            25255..26658
                     /locus_tag="J1Y83_000858"
     CDS             25255..26658
                     /locus_tag="J1Y83_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBA7629034.1"
                     /translation="MSDHNPLTLKLNLREKIAYGMGDVGSNLMLSIGTLYLLKFYTDE
                     LGMPAYYGGIIFLVAKFFTAFTDMLTGFLLDSRKNIGPKGKFRPFILYAAIPAALIAT
                     LQFIATTFSLPVKTTIATALFMMFGLSYSLMNCSYGAMIPAITKNPNERAQLAAYRQG
                     GATIGLLICTVAFIPLQSLFSDSTVGYACAALMFSIGGFIFMMLCYRGVKEHYVDATP
                     TGHKASILKSFCAIFRNPPLLVLCIANLCTLAAFNIKLAIQVYYTQYVLNDINLLSWM
                     GFFSMGCILIGVLLVPLTVKCFGKKQVYLAGMVLWAVGDILNYCWGSNSFTFVMFSCV
                     AFFGTAFVNSLNWALVPDTVDYGEWKTGIRAEGSVYTGYTFFRKISAALAGFLPGIML
                     TQIGYIPNIAQSDATLQGLRQLIFIWPCALAIIAALTMGFFYTLNEKRFALIIEEINQ
                     RKNKEIETEEKTASVTL"
     gene            26726..27418
                     /gene="ompL"
                     /locus_tag="J1Y83_000859"
     CDS             26726..27418
                     /gene="ompL"
                     /locus_tag="J1Y83_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpL"
                     /protein_id="HBA7629035.1"
                     /translation="MKNINAIILLSSLTSASVFAGAYVENREAYNLASDQGEVMLRVG
                     YNFDMGAGIMLTNTYTFQREDELKHGYNEIEGWYPLFKPTDKLTIQPGGLINDKSIGS
                     GGAVYLDVNYKFTPWFNLTVRNRYNHNNYSSTDLNGELDNNDTYEVGTYWNFKITEKF
                     SYTFEPHYFMRVNDFNSSNGKDHHWEITNTFRYRINEHWLPYFELRWLDRNVEPYHRE
                     QNQIRIGTKYFF"
     gene            complement(27521..29344)
                     /gene="typA"
                     /locus_tag="J1Y83_000860"
     CDS             complement(27521..29344)
                     /gene="typA"
                     /locus_tag="J1Y83_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-dependent GTPase TypA"
                     /protein_id="HBA7629036.1"
                     /translation="MIEKLRNIAIIAHVDHGKTTLVDKLLQQSGTFDSRAETQERVMD
                     SNDLEKERGITILAKNTAIKWNDYRINIVDTPGHADFGGEVERVMSMVDSVLLVVDAF
                     DGPMPQTRFVTKKAFAYGLKPIVVINKVDRPGARPDWVVDQVFDLFVNLDATDEQLDF
                     PIVYASALNGIAGLDHEDMAEDMTPLYQAIVDHVPAPDVDLDGPFQMQISQLDYNSYV
                     GVIGIGRIKRGKVKPNQQVTIIDSEGKTRNAKVGKVLGHLGLERIETDLAEAGDIVAI
                     TGLGELNISDTVCDTQNVEALPALSVDEPTVSMFFCVNTSPFCGKEGKFVTSRQILDR
                     LNKELVHNVALRVEETEDADAFRVSGRGELHLSVLIENMRREGFELAVSRPKVIFREI
                     DGRKQEPYENVTLDVEEQHQGSVMQALGERKGDLKNMNPDGKGRVRLDYVIPSRGLIG
                     FRSEFMTMTSGTGLLYSTFSHYDDVRPGEVGQRQNGVLISNGQGKAVAFALFGLQDRG
                     KLFLGHGAEVYEGQIIGIHSRSNDLTVNCLTGKKLTNMRASGTDEAVVLVPPIRMTLE
                     QALEFIDDDELVEVTPTSIRIRKRHLTENDRRRANRAPKDD"
     gene            29717..31126
                     /gene="glnA"
                     /locus_tag="J1Y83_000861"
     CDS             29717..31126
                     /gene="glnA"
                     /locus_tag="J1Y83_000861"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--ammonia ligase"
                     /protein_id="HBA7629037.1"
                     /translation="MSAEHVLTMLNEHEVKFVDLRFTDTKGKEQHVTIPAHQVNAEFF
                     EEGKMFDGSSIGGWKGINESDMVLMPDASTAVIDPFFADSTLIIRCDILEPGTLQGYD
                     RDPRSIAKRAEDYLRSTGIADTVLFGPEPEFFLFDDIRFGSSISGSHVAIDDIEGAWN
                     SSTQYEGGNKGHRPAVKGGYFPVPPVDSAQDIRSEMCLVMEQMGLVVEAHHHEVATAG
                     QNEVATRFNTMTKKADEIQIYKYVVHNVAHRFGKTATFMPKPMFGDNGSGMHCHMSLS
                     KNGVNLFAGDKYAGLSEQALYYIGGVIKHAKAINALANPTTNSYKRLVPGYEAPVMLA
                     YSARNRSASIRIPVVSSPKARRIEVRFPDPAANPYLCFAALLMAGLDGIKNKIHPGEA
                     MDKNLYDLPPEEAKEIPQVAGSLEEALNELDLDREFLKAGGVFTDEAIDAYIALRREE
                     DDRVRMTPHPVEFELYYSV"
     gene            31300..32349
                     /locus_tag="J1Y83_000862"
     CDS             31300..32349
                     /locus_tag="J1Y83_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     NtrB"
                     /protein_id="HBA7629038.1"
                     /translation="MATGTQPDAGQILNSLINSILLIDDNLAIHYANPAAQQLLAQSS
                     RKLFGTPLPELLSYFSLNIELMQESLEAGQGFTDNEVTLVIDGRSHILSVTAQRMPDG
                     MILLEMAPMDNQRRLSQEQLQHAQQVAARDLVRGLAHEIKNPLGGLRGAAQLLSKALP
                     DPSLLEYTKVIIEQADRLRNLVDRLLGPQLPGTRITESIHKVAERVVTLVSMELPNNV
                     RLIRDYDPSLPELAHDPDQIEQVLLNIVRNALQALGPEGGEIILRTRTAFQLTLHGER
                     YRLAARIDVEDNGPGIPPHLQDTLFYPMVSGREGGTGLGLSIARNLIDQHSGKIEFTS
                     WPGHTEFSVYLPIRK"
     gene            32361..33770
                     /gene="glnG"
                     /locus_tag="J1Y83_000863"
     CDS             32361..33770
                     /gene="glnG"
                     /locus_tag="J1Y83_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(I)"
                     /protein_id="HBA7629039.1"
                     /translation="MQRGIVWVVDDDSSIRWVLERALAGAGLTCTTFENGAEVLEALA
                     SKTPDVLLSDIRMPGMDGLALLKQIKQRHPMLPVIIMTAHSDLDAAVSAYQQGAFDYL
                     PKPFDIDEAVALVERAISHYQEQQQPRNVQLNGPTTDIIGEAPAMQDVFRIIGRLSRS
                     SISVLINGESGTGKELVAHALHRHSPRAKAPFIALNMAAIPKDLIESELFGHEKGAFT
                     GANTIRQGRFEQADGGTLFLDEIGDMPLDVQTRLLRVLADGQFYRVGGYAPVKVDVRI
                     IAATHQNLEQRVQEGKFREDLFHRLNVIRVHLPPLRERREDIPRLARHFLQVAARELG
                     VEAKLLHPETEAALTRLTWPGNVRQLENTCRWLTVMAAGQEVLIQDLPGELFESTVAE
                     SPSHMQPDSWATLLAQWADRALRSGHQNLLSEAQPELERTLLTTALRHTQGHKQEAAR
                     LLGWGRNTLTRKLKELGME"
     gene            33882..33992
                     /gene="yshB"
                     /locus_tag="J1Y83_000864"
     CDS             33882..33992
                     /gene="yshB"
                     /locus_tag="J1Y83_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YshB family small membrane protein"
                     /protein_id="HBA7629040.1"
                     /translation="MLESIINLVSSGAVDSHTPQTAVAAVLCAAMIGLFS"
     gene            complement(34225..35598)
                     /gene="hemN"
                     /locus_tag="J1Y83_000865"
     CDS             complement(34225..35598)
                     /gene="hemN"
                     /locus_tag="J1Y83_000865"
                     /EC_number="1.3.98.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HBA7629041.1"
                     /translation="MSVQQIDWDLALIQKYNYSGPRYTSYPTALEFSEDFGEQAFLQA
                     VARYPERPLSLYVHIPFCHKLCYFCGCNKIVTRQQHKADQYLDALEQEIVHRAPLFAG
                     RHVSQLHWGGGTPTYLNKAQISRLMKLLRENFQFNADAEISIEVDPREIELDVLDHLR
                     AEGFNRLSMGVQDFNKEVQRLVNREQDEEFIFALLNHAREIGFTSTNIDLIYGLPKQT
                     PESFAFTLKRVAELNPDRLSVFNYAHLPTIFAAQRKIKDAELPSPQQKLDILQETIAF
                     LTQSGYQFIGMDHFARPDDELAVAQREGVLHRNFQGYTTQGDTDLLGMGVSAISMIGD
                     CYAQNQKELKQYYQQVDEQGNALWRGIALTRDDCIRRDVIKSLICNFRLDYAPIEQQW
                     DLLFADYFAEDLKLLAPLAKDGLVDVDEKGIQVTAKGRLLIRNICMCFDTYLRQKARM
                     QQFSRVI"
     gene            complement(35787..36296)
                     /gene="yihI"
                     /locus_tag="J1Y83_000866"
     CDS             complement(35787..36296)
                     /gene="yihI"
                     /locus_tag="J1Y83_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Der GTPase-activating protein YihI"
                     /protein_id="HBA7629042.1"
                     /translation="MKPSSSNSRSKGHAKARRKTREELDQEARDRKRQKKRRGHVPGS
                     RAAGGNTTSGSKGQNAPKDPRIGSKTPIPLGVTEKVTKQHKPKSEKPMLSPQAELELL
                     ETDERLDALLERLEAGETLSAEEQSWVDAKLDRIDELMQKLGLSYDDDEEEEEDEKQE
                     DMMRLLRGN"
     gene            36878..37510
                     /locus_tag="J1Y83_000867"
     CDS             36878..37510
                     /locus_tag="J1Y83_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418301.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihA family ribosome biogenesis GTP-binding
                     protein"
                     /protein_id="HBA7629043.1"
                     /translation="MTNLNYQQTHFVMSAPDIRHLPSDTGIEVAFAGRSNAGKSSALN
                     TLTNQKSLARTSKTPGRTQLINLFEVADGKRLVDLPGYGYAEVPEEMKRKWQRALGEY
                     LEKRQSLQGLVVLMDIRHPLKDLDQQMIEWAVDSNIAVLVLLTKADKLASGARKAQLN
                     MVREAVLAFNGDVQVETFSSLKKQGVDKLRQKLDTWFSEMQPVEETQDGE"
     gene            complement(37892..40678)
                     /gene="polA"
                     /locus_tag="J1Y83_000868"
     CDS             complement(37892..40678)
                     /gene="polA"
                     /locus_tag="J1Y83_000868"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HBA7629044.1"
                     /translation="MVQIPQNPLILVDGSSYLYRAYHAFPPLTNSAGEPTGAMYGVLN
                     MLRSLIMQYKPTHAAVVFDAKGKTFRDELFEHYKSHRPPMPDDLRAQIEPLHAMVKAM
                     GLPLLAVSGVEADDVIGTLAREAEKAGRPVLISTGDKDMAQLVTPNITLINTMTNTIL
                     GPEEVVNKYGVPPELIIDFLALMGDSSDNIPGVPGVGEKTAQALLQGLGGLDTLYAEP
                     EKIAGLSFRGAKTMAAKLEQNKEVAYLSYQLATIKTDVELELTCEQLEVQPPAAEELL
                     GLFKKYEFKRWTADVEAGKWLQAKGAKPAARPQETSVADEAPEVTATVISYDNYVTIL
                     DEETLKAWIAKLEKAPVFAFDTETDSLDNISANLVGLSFAIEPGVAAYIPVAHDYLDA
                     PDQISRERALELLKPLLEDEKALKVGQNLKYDRGILANYGIELRGIAFDTMLESYILN
                     SVAGRHDMDSLAERWLKHKTITFEEIAGKGKNQLTFNQIALEEAGRYAAEDADVTLQL
                     HLKMWPDLQKHKGPLNVFENIEMPLVPVLSRIERNGVKIDPKVLHNHSEELTLRLAEL
                     EKKAHEIAGEEFNLSSTKQLQTILFEKQGIKPLKKTPGGAPSTSEEVLEELALDYPLP
                     KVILEYRGLAKLKSTYTDKLPLMINPKTGRVHTSYHQAVTATGRLSSTDPNLQNIPVR
                     NEEGRRIRQAFIAPEDYVIVSADYSQIELRIMAHLSRDKGLLTAFAEGKDIHRATAAE
                     VFGLPLETVTSEQRRSAKAINFGLIYGMSAFGLARQLNIPRKEAQKYMDLYFERYPGV
                     LEYMERTRAQAKEQGYVETLDGRRLYLPDIKSSNGARRAAAERAAINAPMQGTAADII
                     KRAMIAVDAWLQAEQPRVRMIMQVHDELVFEVHKDDVDAVAKQIHQLMENCTRLDVPL
                     LVEVGSGENWDQAH"
     gene            40680..>40851
                     /locus_tag="J1Y83_000869"
     CDS             40680..>40851
                     /locus_tag="J1Y83_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA7629045.1"
                     /translation="MSVPVYQIMGKGCHRQDENMSFSPLFVKIFCRSSGSLANRHVDN
                     FVHKLFTSLLIYY"
BASE COUNT         9890 a        10605 c        10219 g        10137 t
ORIGIN      
        1 gccttatccg gcctacagat cgtacatccg gcaacaggca cggacgtcca attaacagga
       61 acacagaaca tgcaaaacat aacccagtcc tggtttgtcc agggaatgat caaagccacc
      121 accgatgcct ggctgaaagg ctgggacgag cgcaacggcg gcaacctgac gctgcgcctg
      181 gatgacgccg atatcgcacc atatcacgac aattttcacc aacaaccgcg ctatatcccg
      241 ctcagccagc ccatgccttt actggcaaat acaccgttta ttgtcaccgg ctccggcaaa
      301 ttcttccgta acgtccagct tgatcctgcg gctaacttag gcatcgtaaa agtcgacagc
      361 gacggcgcgg gctaccacat tctttggggg ttaaccaacg aagccgtccc cacttccgaa
      421 cttccagctc acttcctttc ccactgcgag cgcattaaag ccaccaacgg caaagatcgg
      481 gtgatcatgc actgccacgc caccaacctg atcgctctca cctacgtgct ggaaaacgac
      541 accgcggtct tcactcgcca actgtgggaa ggcagcaccg agtgtctggt ggtattcccg
      601 gatggcgttg gcattctgcc gtggatggta cccggcacgg acgaaatcgg ccaggcaact
      661 gcacaagaga tgcaaaaaca ttcgctggtg ttgtggccct tccacggtgt gttcggcagc
      721 gggccgacgc tggatgaaac cttcggttta atcgacaccg cagaaaaatc agcacaagta
      781 ttagtgaagg tttattcgat gggcggcatg aaacagacta tcagccgtga agagttgata
      841 gcgctcggcc agcgtttcgg cgttacacca ctcgccagtg cgctggcgct gtaaggagca
      901 aacatgatcc gcaaagcctt tgtcatgcag gtaaaccccg acgcccacga agagtatcag
      961 cgtcggcata atcccatctg gccggaactg gaagccgtgc tgaaatctca cggtgcgcat
     1021 aactacgcca tctatctcga caaagcgcgt aatctgctat ttgccacgat agagattgaa
     1081 tctgaagagc ggtggaatgc ggttgccagt acagatgttt gccaacgttg gtggaaatat
     1141 atgaccgacg ttatgcccgc taatgcggat aacagcccag tcagtagcga actgcaagaa
     1201 gtgttttacc tgccttaatg agttgcggtg ccggaaaata tccagcaccg ccctttcatc
     1261 agaagaagta tttaaagcga acgcgtgcgc cgtaacggtc atcactatcg tcgccgtgta
     1321 tcttatcgcc gtcgctatcc agaatggagt aatacgtgcc gaggtaaata ttaaagttct
     1381 ccatatccat cacattagcg aactgataag acgcatgaat ggtgtgaatg ttgtatgttc
     1441 cttcatcatc aatccagcaa tcgttatcac aaaccacgcc actaaattca tcaatcttat
     1501 tatatgcgta gatataaccc agctcgaaac gtttccacag cgcgttaatc ccagcggtga
     1561 aatctttttc attattagca tctaaatagg cggtattaag attaaccacg atgccatttt
     1621 ccggatcggt tttcaggcca ttccaggtca tgctcatgcc ataaccggta cgatcggact
     1681 gatcgacaaa attacccttg ctatcggtat aaccataagc attatttacc acattcgctt
     1741 ccatcgctgc ggaaacggtg aattcttccg tcggcgacca ggcaattacc ggacgcagat
     1801 aagcaacatt tttctgctgt tccatatcgc gtccatgata attaccgtcg ttatataaag
     1861 atgttccgtc ttccagtaac gtgtttaatt caaaatacca gttatcgagt tgtttgctga
     1921 cgaggaaatt accgccagcg ttagaacgcc cgcggccctc tttcatcata tagatataac
     1981 cgctgccatc gtcataaaga tcgttcgcag tattaccgga atgttcaaca aaggtatcct
     2041 gattcagtgg gaacatatcg taggcttcaa aacggcctac tttgaccttc cagtcgtttt
     2101 ctttgccaaa gaagaaaacc gcatcatcga tatttactga gccgtgcata tcccccagcg
     2161 gttgtgcgga gaacccagcg aaatagccat tatccagttt tcgcatacca tcaaaaccta
     2221 acagaatacg accattgaga ttccactgtt cattagtggg atcgctattc acatcagcgt
     2281 tggtcattgc cagcaggcca tgattacttt ccgcatccat attaaattct acgtcaccgt
     2341 agatttttaa atcgccatac cctgaaagca ggagattagg cggggtggcg tttttcttct
     2401 ctgactccgt cgttgattga ccgtttaccg gcgtgttgcc ctgtgcggtt ttaatttcgc
     2461 ggatctcggc ggcttgttgt gtctgaagct gtttaacctg cccctctgct tttgctgccc
     2521 gcatttctgc cgcttccagg cgttcttcca gcaatgccat gcgttgttcc agcgttaaat
     2581 gctgtgtttt tgccataccg gtactacatg ccaataataa accgatggct aaagataatg
     2641 tcgatttttt cattatttac gttccctgaa ataaaggaga ccctacgctt tccaaaagga
     2701 atacgtatat agagaaatat aaggtgtgca ctttcgaatt tttaatccaa cttcagcagg
     2761 ccgatggata gtatcccgcc gcataatatg agtaaatccg ctgatttcct ggaacgtgag
     2821 gaatataaca atagtgccgt ttttaactaa atatcgtaaa aaatattaga tatgattcac
     2881 gctgactcat aagccacaaa tataaataac gcttaaacaa attatctgag acattttaac
     2941 attcacaaaa tcattaaata atgatacttt attcacaaaa tttatccttc actctgcttt
     3001 atattgttac gacttcgtga catatccgtg ctcatcacaa acccgccccg cgattacaat
     3061 taactgaaat atttcatcga ccatttcgtt gtactcgcat aatgctctcg ggaaatgagt
     3121 cttcggccat ccagcttgag tttgcgtcct gaagttgctc tttatcccag tcagagtacg
     3181 ggactttatc tctgatgcgg cgaacgtatt ttttagcctc gagttgatac tggcctgccg
     3241 aattaataaa cgagaaattc atgccgcttt tatccgcttc tttggcctgc gccaccagcg
     3301 tttccagttc agacactttc ttcattgccg cttctgcatc aaacttatct tgcgatatca
     3361 ggttattaat ttgtttcgct gaaatcatga ctgccagaga gtagtagtgg aatgttttcc
     3421 ctttcgcaaa cgcatcgtct ttataatttt cctgggtgta atatttatcc atttcgttaa
     3481 tggtattacc caacgctacg gcagcatcaa tataactcac cgccacgcca tcaataggtt
     3541 gcagcgcagg cgttaacgcc actacacgcg ttttgtttat gaaaaaataa caaaattcac
     3601 gaaatgaata agtcctgcaa tttaatatat atcacaggac ttattttaat taattgctca
     3661 accgctgcgg atgggtataa accgttgccc gacccggttt acaaaagccc accagcgtca
     3721 gattacagcg ctcagccact tctacagcaa gtgtggtcgc ggcagacacc gcaaacaaaa
     3781 tctctacgcc gcacatcgcc gacttttgca ccatttcata actggcacgg ctggaaacca
     3841 gcaccgcacc ttgctgccag ctttcccctt cttgtgaccg gcggcctaac agtttgtcca
     3901 gcgccacatg gcgacccacg tcttcatgcc cgccgaccag ttcgccagat ggcaacatcc
     3961 atgcagcggc gtgagtacaa ccagtcagtt gccccactgg ctggaaatca ttgagatgac
     4021 gtaatgcatc atccagtttg ttgagatcaa acgtctgggt gaacggcagc ggctgcaccg
     4081 gtttaccgat atcgttaagc tgttctacac cgcatacgcc acagcccgta cgtcccgcca
     4141 gcgcccggcg gcgttccttc aaccccataa agcggcggct ggaaagctca atttgtactt
     4201 ccaggccatt acaggaagga accacatcca tgccgaagat atcgcgcggg ctttcgataa
     4261 ttccttcgga aagcgaaaaa ccgagcgcaa agtactcaag gtctttgggt gacgccatca
     4321 tcaccacatg cgaaatgccg ttgtagacca gcgcaacggg aacttcttcc gccacctcat
     4381 ctaaccgtgg gtgttgtaaa tcatcacgcc gccataactc aatttgacga acacccgtga
     4441 tatttgtcac attctcgatt tctttttgct gtgttttttt cactttctct taaccagata
     4501 agaacacaca caccaacatt atggtattct gttacaaacc cttcctggat ggagggaaat
     4561 tgagccaatt ctggaccttt gcggcccctt ccgcaaagaa aaataactcc cactccctgc
     4621 acacgcagca agcgaatgta aatgggacgt gacaatgtcg aaacaaggag caatccatgc
     4681 aggtcagcag aaggcagttc tttaagatct gcgctggcgg tatggcaggc accacggcag
     4741 cggcactggg ttttgcaccc agcgtagcac tcgcggaaac ccggcagtat aaactgctgc
     4801 gcacccgcga aacccgtaat acctgcacct attgttccgt aggctgtggg ctgttgatgt
     4861 acagcctcgg tgacggagca aaaaacgcca aagcatctat cttccatatc gaaggtgacc
     4921 cggatcaccc ggtcaaccgc ggagcacttt gtccgaaagg cgctggcctg gtggatttca
     4981 tccactccga aagccgtctg aagtttccgg aataccgtgc gccaggttct gataaatggc
     5041 aacaaatcag ttgggaagag gcgtttgatc gcatcgccaa actgatgaaa gaagaccgcg
     5101 atgctaacta cattgcgcaa aacgccgaag gcgtgactgt taaccgctgg ctctccaccg
     5161 ggatgctgtg tgcttccgcg tcgagtaacg aaaccggcta tttaacgcaa aaattctccc
     5221 gcgcgctggg tatgctcgcg gtcgacaacc aggcgcgtgt ctgacacgga ccaacggtag
     5281 caagtcttgc tccaacattt ggtcgcggtg cgatgaccaa ccactgggtc gacatcaaga
     5341 atgccaacct cgtcgtggtg atgggcggta acgccgctga agctcacccg gtcgggttcc
     5401 gctgggcgat ggaagccaaa attcacaacg gcgcgaagct gattgtgatc gatcctcgct
     5461 ttacgcgtac ggcggcggtg gctgactact atgcccctat ccgttccggt actgacattg
     5521 ctttcctgtc aggcgtattg ctgtacctgc tgaacaatga aaaattcaac cgcgaataca
     5581 ccgaagccta taccaacgcc agcctgatcg tgcgtgagga ttacggcttt gaagatggcc
     5641 tgttcaccgg ctacgatgcg gaaaaacgca agtacgataa gtccacctgg acttacgaac
     5701 tggacgaaaa cggcttcgcc aaacgtgata ctaccctgca acatccacgt tgcgtgtgga
     5761 acttgctgaa acagcacgtt tcccgttata cgccagatgt ggttgaaaac atctgcggta
     5821 cgccaaaaga cgcgttcctg aaagtctgcg aatacatcgc cgaaaccagt gctcacgata
     5881 aaacagcgtc gttcctctac gccctcggct ggacgcaaca ctccattggt gcgcaaaaca
     5941 ttcgtacgat ggcgatgatc cagctgctgc tcggcaacat ggggatggca ggcggcggcg
     6001 ttaacgccct gcgcggtcac tccaatattc aggggctgac ggacctgggg ctactgtcgc
     6061 agagtctgcc aggttacatg acgctgccaa gcgagaagca gaccgatctg caaacttacc
     6121 ttaccgccaa cacgccgaaa ccactgctgg aaggccaggt taactactgg ggcaactacc
     6181 cgaaattctt cgtctctatg atgaaggcat tctttggtga taaagcgacg gcggaaaata
     6241 gctggggctt tgactggttg ccgaagtggg ataaaggcta cgacgtcctg caatacttcg
     6301 agatgatgaa agagggcaag gtcaatggct atatctgcca gggctttaac cctgttgcct
     6361 cattcccgaa caaaaacaaa gtgatcggct gtctgtcgaa gctgaaattc ctcgtgacaa
     6421 tcgacccgct gaacactgaa acctctaact tctggcagaa ccacggtgaa ctgaacgaag
     6481 ttgactcgtc gaagatccag accgaagtgt tccgtctgcc atcgacctgc ttcgcggaag
     6541 agaacggttc tatcgttaac tcaggtcgct ggttacagtg gcactggaaa ggtgcggacg
     6601 ccccggggat tgcgctgact gatggcgaga tcctctccgg tatcttcctg cgcttgcgca
     6661 agatgtatgc cgaacagggt ggcgcgaacc cggaccaggt gctgaacatg acctggaact
     6721 acgccattcc gcatgagcca tcttcagaag aagtggcaat ggagagcaac ggcaaagcgc
     6781 tggccgatat taccgacccg gcaaccggtg cggttatcgt caagaaaggc caacaactta
     6841 gctcgttcgc ccaactgcgc gatgacggta caacctcctg tggctgctgg attttcgccg
     6901 gtagctggac gccggaaggc aaccagatgg cgcgtcgtga taacgccgat ccgtctggcc
     6961 tcggtaacac gctgggctgg gcatgggcat ggccgcttaa ccgccgcatt ctgtataacc
     7021 gcgcctccgc agatccgcag ggtaacccgt gggatccgaa gcgtcagttg ctgaaatggg
     7081 acggcactaa gtggaccggc tgggatattc cggattacag cgcagcgcct ccgggcagcg
     7141 gcgtagggcc gtttatcatg cagcaggaag gcatgggacg tctgtttgcc cttgataaga
     7201 tggcggaagg tccgttcccg gaacactacg agccgtttga aacgccgctg ggaactaacc
     7261 cgctgcatcc aaacgttatc tcgaacccgg ctgcgcgtat ctttaaagac gacgccgaag
     7321 cattgggtaa agccgataag ttcccgtatg tcggaaccac ctatcgtctg accgagcact
     7381 tccactactg gaccaaacac gcgctgttga atgcgatttt gcaaccagag cagtttgtgg
     7441 aaatcgggga gtcgctggcg aataaacttg gcattgccca gggcgatact gtgaaagtct
     7501 cctccaaccg tggctatatc aaagccaaag cggtagtgac caaacgtatt cgcacgctga
     7561 aagcaaacgg caaagatatc gataccatcg gtattccgat tcactggggc tatgaaggtg
     7621 ttgcgaaaaa aggctttatt gccaatacgt tgacgccatt cgtcggtgat gcgaacacgc
     7681 agacgccgga gtttaagtcc ttccttgtga atgtggaaaa ggtgtaacgg agacgacata
     7741 tggcttatca atcgcaagat atcattcgtc gttccgcgac taacggtctg acccccgcgc
     7801 ctcaggcgcg ggacttccag gaagaagtgg cgaaactcat cgacgttacc acctgtatcg
     7861 gctgtaaagc ctgtcaggtg gcgtgttcag agtggaacga cattcgcgat accgtcggca
     7921 ataacattgg ggtgtacgac aaccccaatg atttaagtgc caaatcgtgg acggtgatgc
     7981 gattctcgga agtggagcag aacgacaaac tggaatggct gatccgcaaa gacggctgta
     8041 tgcactgttc cgatccaggc tgcctgaaag cgtgcccggc ggaaggggca attattcagt
     8101 atgccaacgg tatcgtcgac ttccagtccg agcagtgcat cggctgcggt tattgcattg
     8161 cgggctgtcc gttcgacatt ccgcgcctca acccggaaga caaccgcgtc tacaaatgta
     8221 cgttgtgcgt tgaccgcgtg gtggttgggc aagaaccggc ctgcgtgaag acctgcccaa
     8281 cgggcgcgat tcacttcggt acgaaagagt cgatgaaaac gctggcgagc gagcgcgtgg
     8341 cggagctgaa aacccgtggt tacgacaatg cgggtttgta cgacccggca ggcgtcggtg
     8401 gtacacatgt tatgtacgtg ctgcaccatg ctgacaagcc aaatctgtat catggcttgc
     8461 cggagaaccc ggaaatcagc gaaaccgtga aattctggaa aggcatctgg aaaccgctcg
     8521 cagctgttgg ctttgcggct accttcgcag ccagtatctt ccactacgtg ggtgtcggtc
     8581 cgaaccgtgc ggatgaggaa gagaataatc tgcacgaaga gaaagacgag gagcgcaaat
     8641 gaaacgacgt gacaccatcg tgcgctacac cgcgccggaa cgtatcaacc actggatcac
     8701 cgccttctgc ttcatcctgg cggcggtgag cgggctgggc tttttgttcc cgtccttcaa
     8761 ctggttgatg caaatcatgg gcacaccgca gctggcgcgc attctgcacc cgtttgtcgg
     8821 cgtggttatg tttgcctcgt tcatcatcat gtttttccgt tactggcatc acaacctaat
     8881 caatcgggat gatatctttt gggcgaagaa tattcgtaag atcgtcgtca acgaggaagt
     8941 aggtgacacc gggcgttata acttcggtca gaaatgcgtt ttctgggcgg cgattatttt
     9001 cctggttctg ctgctggtga gcggcgtgat tatctggcgt ccttattttg cgcctgcttt
     9061 ctcaatcccg gtgatccgat tcgcgttaat gctgcattca tttgccgcag tggcgttaat
     9121 tgtggttatc atggtgcata tctacgccgc cctttgggtg aaaggcacga ttaccgccat
     9181 ggtggaagga tgggtgacca gcgcatgggc aaagaaacat cacccgcgct ggtaccgtga
     9241 ggtccgcaag acaacggaaa agaaagctga atgagtattc gcataatccc gcaagatgag
     9301 ctgggttcga gcgagaaacg tacggcggat atgattccgc cgttattgtt ccctcggctc
     9361 aagaatttat acaaccgccg cgccgagcgt ctgcgcgagc tggcagaaaa taatccgctg
     9421 ggtgattacc tgcgctttgc tgcgcttatc gcccacgccc aggaagtggt gctgtacgac
     9481 catccgctgg agatggatct gaccacgcgc attaaagaag ccagcgcaca aggcaagccc
     9541 ccgctggata ttcacgttct gccgcgtgat aagcactggc aaaagctgct gatggcgctg
     9601 attgctgagc tgaaacctga aatgagcggc ccggcgctgg cagtgattga gaatctggag
     9661 aaggcatcga ctcaggagct ggaagatatg gccagcgcac tgtttgcctc tgatttctca
     9721 tccgtcagta gcgataaagc gccgtttatc tgggctgcgc tgtcgctcta ttgggcgcag
     9781 atggctaatc tgatccccgg caaagcccgc gctgaatacg gcgaacaacg tcaatattgc
     9841 ccggtatgtg gctctatgcc ggtgtccagc atggtgcaaa ttggcaccac tcagggtctg
     9901 cgttacctgc actgcaacct gtgtgaaact gaatggcacg tagtacgcgt aaaatgcagc
     9961 aactgtgaac agagcggcaa actgcattac tggtcgctgg atgacgaaca ggccgcgatt
    10021 aaagccgaaa gctgcgatga ctgcggcact tacctgaaga ttctttatca ggaaaaagaa
    10081 ccgaaagttg aagccgtggc agatgacctc gcctctctgg tactggacgc gcgaatggag
    10141 caagaaggct atgcccgcag ttccatcaac ccgttcctgt ttccgggtga aggggagtaa
    10201 tctctgacgt tttccgggtg gcgctggtcg cccggattac agctcatagg gtgtgacgac
    10261 acctgctatt cccgcaaaat ctctcgtatt tcgcgaggcg ttttccagat atgatgtaac
    10321 agttgcagtt attataagtt caagtaaaac aaccttgtat taataccaga ataaggtcat
    10381 ctggtttttt gtttttatat tcatgaataa aaaaactttt gtcattcctt atgctgctta
    10441 catagttctt caacaccaac tgcctcgtcc ctttgcctgc cgccttttgc cttacgcacc
    10501 ggaaattgct gccgctccag atattgctct accgcttcgc gtaaaattgc agatcgtgaa
    10561 accttacgcc agcccctgag tttattcagc cgtttaattg cctcttctga taaatgaaga
    10621 aaaaccgtat tcatcgccat taccaccacc ttacatatat tactcattaa tttatgtgcg
    10681 aaaattacgc cggaaagtta attaacgaaa gcggattttt tcatattgtt ttgtttttgc
    10741 gagacgcttt ccggattttt cacgcccgtg aatttccctg tttccatcca gcaaaaacgg
    10801 gcgtataagg ctttgcattc gtacagcaaa atgagttctc caatggcact ggaaaaagga
    10861 attgcagagt ttatcaccac ggattttttc agcgaggcag cgcgtacggc tctacgggat
    10921 gtgcgcctgg agagcaggta ggtaactccg gatccagata aaattgtttt caacttgaaa
    10981 atatcacagg cctgccttac agtgttttca ggttgaaaac tcgtaatgga attacaccat
    11041 gcatgtcatc agccacaagc cttttaacga ggcgataaaa cgctttccac cttacgaagg
    11101 tgcacttatt gatttattaa atcttcttga gaaaacagag tttagcgatc cgggtgagat
    11161 gaagagatat attccctcac tggataattt taaatacaga gataaatggt gggttattaa
    11221 tattgcaggt aatcatttac ggcttatcgc atatattgat ttcaggctcc aaaaatgttt
    11281 tatcaaacat actgttatct acaatgaata cgacagattc acaacctagt accggagcca
    11341 taaaaatgag aacccatcac accccagcag aagactcggc caattttgga atgctggtta
    11401 agaccttcac ccacgcagtg aaagacatcc ctttatgtgg ccctaaagcg gatcaagagg
    11461 aataccataa tgcgctgaaa ctgatcgaat ttttagtcga tcgtgacgat ctggaaaacc
    11521 ctttatttga attactctgc gtgcggatcc gcgaatatga aaataacgct ccagaatttc
    11581 agcattttaa tcagagatta gcatcgaccc cgtgcggtgt aagtctgtta cggatcctta
    11641 tggatcaaca tgggcttaaa gcggctgatt tggcacaaga acttggctct aaatctaatg
    11701 tgagtaatat tcttaatggt cgccgggcgc tcaccgttcg ccatatcaag gcgttaagcg
    11761 agcgttttaa tctcccggct gaagcgttta ttgataagta aaatctccac cctactcttc
    11821 ttcgtttccg ccctcttcat acggaccaaa tggcttcgcg ggcagaacga tatacgtgcc
    11881 ttcaaacact gcacccggcg tctcgtcgcc aaagatttcg acctgcatct gcacccgtgc
    11941 ttttcgtccg cgcgccagac ggtcgagatc gccacttaag gcaccaaggt cggctaccgc
    12001 atggggtttt ccgctaatcg gtttgctata gcggatatgc gcatccgcca gaataatcgt
    12061 tccgccgagg tggcgttcgc gcagcatcag ccagataagc ccccaaccag tgagcgttgc
    12121 cagcgagaat aaactcccgg caaacagcgt gtgatgcgga ttctgattgc ctgtttccgg
    12181 catggtggtg ataaattttt gcccggtata ttgctgaatg cgcacaccca ttttttcact
    12241 cagcgggatg tgttcatacc acgcttgctg gagctgggcg caccagtcgc cgcgatgaag
    12301 aatatcatcc agagtggcaa caggttttat catcaaaaaa tggcgaatcg gcgtggtggt
    12361 tggcgtggtg atttcaccct gattaacaaa ccccagcttg gcgaaaaact ccgccgcgtc
    12421 ttcacgggcg ctacaggtca cgcgcttaac gccttcctga cgtgccaccg actccagggt
    12481 catcgccatc agcgtgccta accctttgtc ctgcacgtcg ggatgaacgg ccataaagcg
    12541 aatggacgct tcattgtcgg cattaatata cagtcggcct accgctacca gattaccctg
    12601 ctcgtcgacg accatctggt gatgcgccat cgcatcccac gcgtcgcgtt ccgaaccttt
    12661 tggttgatgc aggggcttac gcaacatttc ccagcgaaac tgatagtaac gctctaattc
    12721 ttcttctgtt tgtggaaccc gaaggtgata catagcgata ctctctcttg tttcccgtga
    12781 caaccctgga agctggctca tacctgcaac cagaatgtca cggggccatc attgaccagc
    12841 gatacctgca tatccgcagc gaagcgtcct gtttgcgtgt tcatctcttg ctgacggcag
    12901 cgttcgacga aatagtcata taacgcctct gcgcgatccg gtgatgcgcc tttggagaaa
    12961 cttgggcgca tcccccgttc ggtatctgcg gcaagggtaa actgggaaac caccagtaca
    13021 ctgccgcccg cctgttgcac gttgagattc atcttgcctt cggcatcgct aaagatgcgg
    13081 tagccgagca cacgctcgca cagacggttt gctttctgtt cgtcgtcatc cttttcgaca
    13141 cccaataaca ccaaaagtcc cgcgccaatt tcacccgtca cttctccctc cacggtgacg
    13201 ctggcacggg ttacgcgttg aattaatgca atcatggttc gtcgtcttct tcttgttcag
    13261 cagcttgttt taatttgcgg tattccccga gagtgacagt aatttccgcg ccaagcaaga
    13321 cgatacacca tgtccagtag acccaaacaa agagaatggg gatcaccgcc agcacaccgt
    13381 aaatgagctg atatgatggg aacatggtga tataaagcgc gaaacctttc tttcctgctt
    13441 cgaacaggag cgcggcgaca aacgcgccga caatcgcgtc gcggttaggt acacggatgg
    13501 taggaacgat gctgtacagc agccagaaag agatccacga caacagcagc ggaaaaatac
    13561 gcaacacatt atcgatgaca gtattgagat cgctcgccca gcgcagagag agcagatagg
    13621 agctgatcgc cagactggcc cctgccaaca gcggccccag cgttaaaatc atccagtaca
    13681 cggcaaacga gtaaattttg ggtcgcgctc gtttactgcg ccagatggta ttcaacgcgc
    13741 tatcgatgga gtacatcaac aataacgccg tgacgatcag cccgcacgcc ccgacggcgg
    13801 tcattttgtt ggaattggcg acaaactgtt cgatatatcg ctggataaca tcgccagtag
    13861 caggcagaaa gttggcaaaa ataaagtgac gcaactgaat gctgacgtcg gaaaacatgg
    13921 gaaaagcggc gaaaagcgca aaaacaacgg caaccagcgg cactaatgag agcaacgaca
    13981 cataggcaag gttacctgcc agggttgtca tgttgtcctc atcaatgcgt tgccagagta
    14041 gttttagcca ggcccatagt ggacgggtac gatgcctggc tttgtcctga atggttttta
    14101 gcataacacc ttcgcgaaat agtccgggat ggtggtttta tctttcacca gaatactagt
    14161 aatgcctagc cgattagctc cttctatatt atcggcgtta tcgtcgaaaa agaccgtatc
    14221 gtctggtgaa aaaccttctg cctgcaaaac atgctgataa attcgtgctt caggtttgcg
    14281 catacccaag tcttgcgaca gatagatatg gtcagcagca tcacggattt ccgggtattc
    14341 ctccggccag aaggtagtat gcaggcggtt agtattggaa agcaccacca cgcgatgccc
    14401 ctgctcacgc agtttatgca tgatggcgat cacttccggt cgtagcgcaa caaataccgc
    14461 ctgccaaccg tgggagaact gctcatagct tagcggcagg gccatctcat gacacagtgc
    14521 ctctgcgaac gcttcgtcgc taatttcccc acgctcatgc tgatgaaacg cctcgcccat
    14581 gtggaaactc ttcttaagcg tagccagtgg aacacgcgtt aaatcgctcc aggcccccag
    14641 cacacggtta aagtcgatat cgacaatcac attacctaaa tcaaagatat agagcatttt
    14701 tgcctccttt cgcgccacaa gaaattaact gtagcgggaa aggatgggtt tggctatgtg
    14761 gccaagtgtg aagccaggtc aacggtcaca aaattctacg cgtcttcctg gggaaggatg
    14821 atttcaattt ggctgtcttg taagcgtgat tttagcgcag cgggcggcag ctcgtcggta
    14881 aagagcgcag tgacctgtgc cacgttacca atttcaaccg ccgccgaagc atgatactta
    14941 gtgtgatcgg cgaccagcag gatatttctc gcgtgcgcca tcatcgtttt caccacgtta
    15001 gcttcgttaa catcaaactc catcaacgcg ccatcgctct cgattgcgcc tacgcttgtt
    15061 accagataat ctgcgcgaaa atcggccaca aaggacgccg ctgaagggcc aatgatccca
    15121 ctgttatggg aacgcagcgt tccgccaggc accatcactt caaagcgcgg gttgtggtaa
    15181 agaatatgcg ccacacgcag gctgttggtg attatccgca aatggttatg gttaagtaac
    15241 gcccgggcaa catgctcaac ggtcgtacca atggtgataa atattgttga accatcagga
    15301 atataatctg ccaccgcttc ggcaatcgct tttttttctt cggtttgcga aacctcacgc
    15361 tgctcgaacg ccgtattaac gacgctggaa gcccgacccg cgccaccgtg atggcgcgta
    15421 atcagtcctt gctcgcttaa tttacgaata tcccggcgca ccgtctgcgt ggagacatcc
    15481 agcagatttg ccagctcatc aatattcata tacccacgct cggcgatcag catgaggagt
    15541 tggtcgtgcc gcgggttacc ggttagttcg gtaaggctca tgaaaattcc tcgaaaaacc
    15601 atcaccctgg cattttatac aaaaagtgac aaaaaagatc gggtttgatc acagtcaggg
    15661 atcccagctc gtccaccggg acgtgtgcat tttaacgccg ctacaccgct ggcgaagcgg
    15721 actgactccg ctaaatcccc acctgtggcc agcgccaccg ccaaagcgcc gtgaaaaaca
    15781 tcacccgctc ctgtggtatc taccacatca actttgaagg ccggttgatg ttgacgcccg
    15841 ccattttcca gccagtcgca gcctgcgctg ccctgggtca catagacatg accatttgtg
    15901 agcgtttgtg cctgttttag ggcactcgcc atctctttca cgcccgttaa gcgcgccaga
    15961 cccggttctg aaaaagctgc atgatcgctt aacgccacca gttcgctgat atcctgcggc
    16021 gtaatatcac cgtccagcac ggtcattacg cccgcctgcc gcgccagagt gaaggctttt
    16081 ttcgcgccgt cgtgccagcg tacatctgcc agcaccacgt cccactgaga gaaatcaatt
    16141 tcctccaacc actctgcatc aggcagtagg tctgggctgg gatagttaat gatgatgcgc
    16201 tcacctttgg catccaccat gatggcggat tgcgaagatt tcgcctggtt ataccgtttg
    16261 gtgtaacggg tgttaacacc ccaggattcc agctctgcca gcaggctgtt gcccgtgtcg
    16321 tcgtcaccga cgcgaccaat aaaatcgacc tgcgcaccta gtctggccgc cgcaactgct
    16381 gctgtagccg ctggcccgcc gccaacttcc gtataatttt tcgccacgta tttaccgctc
    16441 tccgtcggca atccttccac gtaatagatg cgatccatca cggtaattcc tacacaagca
    16501 acacgaatca tggtcattcc ttaagcattt tgggatgcgt tcattttaat ttcatcaatt
    16561 gtttaaaaag tgacggctgt caattttttg acgataaatt acaaatacga tcaaaaacag
    16621 acaagcaagg catgtcataa atgacaaaaa gtgacatcac tgtattcagg agagagttat
    16681 ggcagcaatc gcgtttatcg gtttagggca aatgggttcg ccaatggcga gcaatttatt
    16741 gcagcaaggg catcaacttt gcgtcttcga tgtgaatacc gaagccgtac agcatctggt
    16801 cgacaaaggg gcgactcccg ctgctaaccc agcgcaggcc gctaaaggtg ccgaatttat
    16861 cattaccatg ttgccgaatg gcgatctggt gcgtcacgtg ttgttcggtg aaaacggtgt
    16921 ctgtgagagc ttatcttccg atgcgctggt cattgatatg tcgaccatcc acccgctgca
    16981 aaccgataaa ttgattgctg atatgcaggc cagaggcttc agcatgatgg atgttccggt
    17041 aggccgtact tccgcaaatg ccattaccgg tactctgcta ctgctggctg gcggcactgc
    17101 tgaacaagtt gaacgcgcca cgccgatcct gatggcgatg ggcagcgagt tgatcaacgc
    17161 gggcggtccg ggcatgggga tccgcgttaa gcttatcaac aactacatga gcatcgcgct
    17221 caatgcgttg tcggcagaag ccgccgtttt gtgcgaagcc ctcaatcttc ccttcgatgt
    17281 tgccgtcaag gtaatgagcg gtactgccgc cggtaaaggc cacttcacca cttcctggcc
    17341 gaacaaagtc ctcagcggag atctttctcc cgccttcatg atcgatcttg cccataagga
    17401 tcttggcatc gcccttgatg tcgccaacca gttgcatgtg ccgatgccac tgggggccgc
    17461 ctcacgggag gtttatagcc aggcgcgcgc agcgggtcgc ggtcgccagg actggtccgc
    17521 cattctggag caggtccgtg tcagtgccgg gatgactgcc aaagtaaaaa tgtaacgact
    17581 ggataaagga ataaatgaat gaataagtac accatcaacg acattacgcg tgcatcgggc
    17641 ggttttgcca tgctggctgt cgatcagcgc gaagccatgc gcatgatgtt tgccgcggct
    17701 ggcgcacccg ctccagtagc caatagcgtt ttaactgatt tcaaagttaa cgccgcgaag
    17761 actctttcac cttatgcatc ggcgattctg gtagatcaac aattctgcta ccgccaggtg
    17821 gttgagcaaa acgcgattgc caaaagttgc gccatgattg tcgccgccga tgagttcatt
    17881 cctggcaacg gtattccggt agatagcgtg gtcattgacc gcaaaatcaa tccgctacaa
    17941 atcaaacaag acggtggcaa agccttaaaa ctgctggtgc tgtggcgtag tgatgaagat
    18001 gcgcagcaac gcctggatat ggtgaaagag tttaacgaaa tgtgccactc acacggtctg
    18061 gtaagcatca ttgagccagt cgtccgccca ccgcgtcgtg gcgataaatt cgatcgcgaa
    18121 caagcaatca tcgatgcagc caaagagctg ggcgacagtg gcgctgatct ctacaaagtt
    18181 gaaatgccgc tttacggtaa aggtccgcaa caagagcttc tcagtgcttc acaacgtctg
    18241 aatgaccata tcaatatgcc gtgggtgatc ctctcttccg gtgtcgacga aaaactgttc
    18301 ccgcgtgccg tacgcgtggc aatgacagcg ggtgcatcgg gcttcctggc tggtcgtgcc
    18361 gtctgggcat cggtcgtcgg tttaccagac aacgagctga tgctgcgtga cgtttgcgca
    18421 ccgaaattac aacaacttgg cgatatcgtc gacgaaatga tggctaaacg ccgttaagat
    18481 ggggatacaa aaatgaaatg gtttaacact ttaagtcaca accgttggct ggaacaggaa
    18541 accgatcgca tctttgattt tggtaaaaaa tccgtagtac cgaccggatt tggctggtta
    18601 ggcaataaag ggcaaatcaa agaagagatg ggcacccatc tgtggatcac agctcgtatg
    18661 ttgcacgttt attccgttgc tgcggcgatg ggccgacctg gcgcttactc gttggttgat
    18721 cacggtatca aagccatgaa tggcgcactg cgcgataaaa aatatggcgg ttggtatgcc
    18781 tgcgtgaatg acgagggcgt ggtggatgcc tccaaacagg gctatcaaca tttctttgct
    18841 ctgctgggtg ctgccagcgc cgtcactacc ggtcatccgg aagcgcgcaa gctgctcgat
    18901 tacaccattg aaattatcga gaaatacttc tggagcgaag aagagcagat gtgcctggag
    18961 tcctgggatg aagccttcag caaaaccgaa gagtatcgcg gcggcaatgc caatatgcac
    19021 gcggtggaag ccttcctgat tgtctatgac gtcacccatg acaaaaaatg gctggatcgc
    19081 gcgattcgcg tggcttccgt gattatccac gacgtcgcca gaaataatca ttatcgcgtt
    19141 aacgaacact tcgataccca gtggaatccg ctgccagatt acaacaaaga taacccggcg
    19201 caccgcttcc gcgcgttcgg aggtactccg ggccactgga tcgaatgggg ccgtttaatg
    19261 ctgcacattc acgcggccct ggaagcccgt tgcgaacaac cgccagcatg gctgctagaa
    19321 gatgccaaag gtctgtttaa cgccaccgtg cgcgatgcct gggcacccga tggcgcggac
    19381 gggattgttt ataccgttga ctgggaagga aaaccggtgg tccgcgaacg tgtgcgttgg
    19441 cctatcgtcg aagcgatggg tacggcctac gcgctctaca ccgtcactgg cgatcgccag
    19501 tacgaaacct ggtatcagac atggtgggac tactgcatca aatacctgat ggactacgaa
    19561 aatggttcat ggtggcagga gctggatgcg gacaataagg tcaccaccaa agtctgggac
    19621 ggcaaacagg atatttatca tctgctgcat tgtctggtga tcccacgtat cccgttagcg
    19681 ccaggcctgg ctccggcggt tgcggcgggt ctgctggata ttaacgcgaa ataactgact
    19741 aaaaaaacga catatcaggc tgcacgccgg gtatgtcgtt attcaataaa ggtacctcgt
    19801 atgcaaatca ccaacatgca ttgcagtgga cagaccgttt cacttgccgc gggggattat
    19861 cacgcgacca ttgtgaccgt cggtgcaggt ctggctgaat taacctttca gggctgtcat
    19921 ttagttattc cgcataaacc cgaagagatg ccgctggcac atctgggcaa agtccttatt
    19981 ccgtggccaa accggatcgc caacggctgt tatcgctatc aggggcagga atatcagtta
    20041 cccattaatg aacatggttc aaaagccgct attcatgggc tgctcgcctg gcgtgactgg
    20101 caaatcagcg aattaagcgc caccagcgtc acattaaccg cctttctgcc acccagttac
    20161 ggctacccgt ttatgctggc ttcgcaggtg gtttattcgc tcaacgcccg cacgggtctt
    20221 tccgttgaga tcgcaagcca gaatatcggc actgtcgcag cgccttacgg tgtgggtatt
    20281 cacccttatt taacctgcaa cttaacatct gttgatgaat atctgttcca gcttccggca
    20341 aaccaagttt atgccatcga tgagcacgct aatccgacaa cgcttcacca cgttgatgag
    20401 ctggatttaa atttcacgca ggcgaaaaaa atagccgcga caaaaatcga ccacaccttt
    20461 aaaaccgcta atgatttgtg ggaaatgact attacccatc cacagcaggc gctttcggtg
    20521 agcctctgtt ccgatcaacc ctgggtgcag gtttatagcg gtgaaaaatt acaacgtcag
    20581 ggactggcag ttgaaccaat gagttgcccg cctaacgcct ttaattcagg tatcgactta
    20641 ttattactgg aaccagggaa aacgcaccgc ctgtttttta atattcacgg gcagcacaat
    20701 taattttatt aagaaaagaa cgagatcatt gtttagagtt gtatttacac aacaggaaat
    20761 attatggact tttttacgtt attttcaccg gatggggtca tgctggaaac aaacgtggta
    20821 tgtatcgtta tcgcgttaat gtttctttat acctttgtgg ggatatgcgc gggatttggc
    20881 ggtggtttaa ccaccatgcc gctcgtcacg ttaatgctcc ccgtaaaaat ggcaactccg
    20941 ctctcggtga ttgtcggcac cgcgaccgcc ttgtacgcca cctggctgtc acgtaaagaa
    21001 acggattggc gatcggcaat ggtactgatt attttctcgt ttctgggaat acccgtcggt
    21061 ttatatgcgc tgtcgtattt acccgaccat tttatgaaag tgggcttagg cggattcctt
    21121 atcctgtact ccttttacag tatgtttatt ccacgcctgc cgatttatga ccgtagctgg
    21181 atcgccgcac ctatcggcgc cgtggcagga gcgttagggg cagcattttc aaccaacgga
    21241 ccgccgatgg ttatctacgg tatgctgcgt aacttaggcc cggcggcatt tcgcggcact
    21301 ctgaacgctt tcttcaccgc aaacaacatc gcggtgatcg gcgggctggc gaccagcgga
    21361 attttgactg tctctatctt taaactggtg ctgttttgta ttcccaccat gatcctcggt
    21421 tcactggttg gacaatatgt gcataaacgc atttcggtca aagtgttccg tgtgatggtc
    21481 tttttattgc tgattgcctc cggttctatg ttgataaaag gcgcgctggg aattcccgcc
    21541 gctgccgcac ttcttcctcc gtttaccttg ttagttatac tgcaactggc atttggtaaa
    21601 aaagtggcgg caatccgtcg ggcggaagaa agtaaataat cattatgcgg gttttaattt
    21661 atacccgcac acattcttta attaatgaaa cctgtaataa caggatggca tgaaatatta
    21721 gttttatatc tatggagatt atttatggat acgccacgcc cacagctatt agattttcaa
    21781 tttcatcaga ataacgacag ttttactcta cgttttcaag accgccttat tttaatccat
    21841 agcaaagata atccttgttt atggattggt tcaggtatag cggatatcga tatgttccgc
    21901 ggtaatttca gcattaaaga taaattgcag gagaaaattg cgcttaccga cgccatcgtc
    21961 agccagtcac cggatggctg gttaatccat ttcagccgtg gttctgacat tagcgccacg
    22021 ctgaatatct ctgccgacga tcatggccgt ttattgctgg aactacaaaa cgacaacctt
    22081 aaccacaacc gcatctggct gcgccttgcc gcacagccgg aagatcatat ctacggctgc
    22141 ggcgaacaat tctcctactt tgatctgcgc ggcaaaccgt tcccgctgtg gaccagtgaa
    22201 caaggcgttg gtcgcaacaa acaaacctat gtcacctggc aggccgactg caaagagaat
    22261 gcgggagggg attactactg gaccttcttt ccacaaccca cgtttgttag cacgcagaag
    22321 tattactgcc atgttgataa cagttgctat atgaacttcg actttagcgc gccggaatac
    22381 catgaactgg cgctgtggga agacaaagca acactgcgtt ttgaatgtgc tgatacatac
    22441 atctccctgc tggaaaaatt aaccgcgctg ctgggacgcc agccagaact gcccgactgg
    22501 atttatgacg gagtaacgct cggcattcag ggcggcactg aggtgtgcca gaagaaactg
    22561 gacaccatgc gtaacgcggg cgtgaaggtc aacggcatct gggcgcagga ttggtccggt
    22621 attcgtatga cctcttttgg caaacgcgta atgtggaact ggaagtggaa cagcgaaaac
    22681 tacccgcaac tggattcacg tatcaagcag tggaacaaag agggcgtaca gttccttgcc
    22741 tatatcaacc cgtatgttgc cagcgataaa gacctctgcg aagaagcggc aaagcgcggc
    22801 tatctggcaa aagatgccgc cggcggcgat tatctggtgg agtttggcga gttctacggc
    22861 ggcgttgtcg atctcactaa tccggaagcc tacgcctggt tcaaggaagt gatcaaaaag
    22921 aacatgattg aactcggctg cggcggctgg atggctgact tcggcgagta tctgcccacc
    22981 gacacttacc tgcataacgg cgtcagcgcc gaaattatgc ataacgcctg gcctgcgctg
    23041 tgggcgaaat gtaactacga agcccttgaa gaaacgggca agctcggcga gatcctcttc
    23101 tttatgcgcg ccggttccac cggtagccaa aaatattcca ccatgatgtg ggcgggcgac
    23161 cagaacgttg actggagtct ggacgatgga ctggcgtctg tcgtcccggc ggcgctgtcg
    23221 ctggcaatga ccggacatgg cctgcatcac agtgatattg gcggttacac caccctgttt
    23281 gagatgaagc gcagcaaaga gctgctgctg cgctggtgcg atttcagcgc cttcacgccg
    23341 atgatgcgca cccatgaagg taaccgtcca ggcgacaact ggcagtttga tagcgacgcg
    23401 gaaactatcg cccattttgc ccgtatgacc accgtcttca ctaccctgaa accttacctg
    23461 aaagaggccg tcgcgctgaa tgcgaagtcc ggcctgccgg ttatgcgccc gctgttcctg
    23521 cattacgaag acgatgcaca gacctacacc ctgaaatatc agtacctgtt aggccgcgac
    23581 attctggtcg ctccggtgca tgaagaaggc cgtagcgact ggacgctcta tctgccggag
    23641 gataactggg tccacgcctg gacgggtgaa gcgttccggg gcggggaagt gaccgttaat
    23701 gcgcccatcg gtaaaccgcc ggtcttttat cgcgccgata gcgaatgggc ggcactgttc
    23761 gcgtcgttaa aaagcatcta atcacatccg cccgtgggga aactcacggg caaagggaga
    23821 acaacgatga gtcacatcac aacggaagat ccagcaactc tacgcctgcc ctttaaagag
    23881 aaactctctt acggtatcgg cgatctggcc tctaacatcc tactggatat cggcacgctt
    23941 tatcttttga agttttatac cgatgttctg gggctgcctg gcacctatgg cggcattatc
    24001 tttttgattt caaaattctt taccgccttt accgatatgg gtaccggcat tatgttggat
    24061 tcccgacgca agatcggtcc aaaaggtaag ttccgtcctt ttattctgta tgcgtcattt
    24121 ccggtcaccc tgctggcgat cgccaacttt gtcggcacac cgtttgatgt caccggtaaa
    24181 acggtgatgg ccaccatcct gtttatgctt tacggactgt ttttcagcat gatgaactgc
    24241 tcgtatggcg cgatggtccc cgctattact aaaaacccca acgaacgcgc ctcactggcg
    24301 gcatggcgtc agggtggcgc tacattgggc ctgctgcttt gtacggtagg attcgtgccg
    24361 gttatgaatc ttatcgaggg taatcagcaa cttggctata tcttcgccgc cacgctgttt
    24421 tcactgttcg gcctgctgtt tatgtggatc tgctattcgg gcgtgaaaga gcgttatgtc
    24481 gaaacccaac ctaccaatcc ggcgcaaaag cctggcttgt tgcaatcttt ccgcgcaatt
    24541 gcggggaacc gcccactgtt cattttgtgc attgccaacc tctgtacctt aggggcgttt
    24601 aacgtcaagc tcgccattca ggtctattac acccagtacg tgcttaacga tcccatcctg
    24661 ttgtcatata tgggattttt cagcatgggc tgtattttca tcggcgtgtt cctgatgcct
    24721 ggtgcagtca gacgttttgg taagaagaag gtctatatcg gcggtctgct gatctgggtg
    24781 ctgggcgatc tgctcaacta tttcttcggc ggcggctcgg tcagctttgt ggctttctcc
    24841 tgcctggcgt tctttggttc ggcgtttgtt aacagcctga actgggcgct ggtttctgac
    24901 accgtcgagt acggcgagtg gcgcaccggt gtgcgctcag aaggtacggt ctacaccggt
    24961 ttcaccttct ttcgcaaagt atctcaggcg ctggcaggtt tcttcccagg ctggatgctg
    25021 acgcaaattg gctatgtgcc aaacgtcgcc caggctgacc acactatcga agggttgcgc
    25081 caactgatct tcatctaccc aagcgcactg gcagtagtca ccattgtggc aatgggttgc
    25141 ttctacagtc tgaacgagaa gatgtacgtc cgcattgttg aagagataga agcccgtaaa
    25201 cgcacggcgt aattataata aacaacgccc tgcggggcgt tataaggagt gattatgtct
    25261 gaccataatc cactgacatt aaaactgaat ctgcgggaaa aaatcgccta tggtatgggc
    25321 gacgtcggtt cgaatttaat gcttagcatc ggcactctgt atctcctcaa attttatacc
    25381 gatgaattag ggatgcctgc ttactatggc gggattatct ttctggtggc gaaattcttt
    25441 accgcattta ccgatatgct caccggattt ttactcgact cgcggaaaaa tattggacct
    25501 aaaggaaaat tcaggccgtt tattttatac gcagcgattc cggcggcgtt aattgcaacg
    25561 ctccagttta tcgccaccac ctttagtttg ccggttaaaa cgacgattgc caccgcactg
    25621 tttatgatgt ttggactttc atacagtctg atgaactgct cgtatggcgc gatgatcccg
    25681 gccattacca aaaacccgaa tgagcgcgcg caactcgctg cttaccgcca gggtggtgca
    25741 accatagggc tattgatctg taccgtggcg tttattcctt tgcagtcact attttctgac
    25801 tccaccgtcg gctacgcctg cgcggcactg atgttctcca ttggcggctt tatttttatg
    25861 atgctgtgct acagaggtgt caaagagcat tatgtggacg cgacgccaac cggacataag
    25921 gccagtattc tcaaatcatt ttgcgcgata tttcgtaatc cacctttgct ggtattgtgc
    25981 attgctaacc tgtgtaccct ggcggcattt aatatcaaac tggcgattca ggtctattac
    26041 acccagtatg tgctgaatga tattaactta ttgtcgtgga tgggattctt tagtatggga
    26101 tgcattctca tcggcgtatt actggtgcca ctaactgtaa aatgttttgg taaaaaacag
    26161 gtttatctgg ctggcatggt gctgtgggcg gtgggggata tcctgaatta ttgctggggc
    26221 agtaactcat tcactttcgt catgttctct tgtgtcgcct tttttggcac ggcctttgta
    26281 aacagcctga actgggcact ggtaccagat accgtagatt atggtgaatg gaaaacaggt
    26341 atacgtgccg aaggttctgt ttataccggt tataccttct ttcgtaaaat ttcagccgca
    26401 cttgctggct tcttgcccgg cattatgctg acgcaaatcg gttatattcc caacatcgcg
    26461 caaagcgatg caacattaca gggtttgcgt cagctcattt ttatctggcc ttgtgcgctg
    26521 gcaattattg ctgcattgac gatggggttc ttttacacgc tcaatgagaa acggtttgca
    26581 ctgattattg aggaaattaa ccaacgcaaa aataaagaaa tcgagacaga agaaaaaacg
    26641 gcttccgtaa cgttataaat gtcaatagcc gctatttcca tcctgatggg tggcggcctc
    26701 cctacgttta aaaaatggac ttattatgaa aaatattaat gcaataattt tattatcttc
    26761 tctgacctct gcctcggtat tcgctggcgc atacgtcgaa aacagggaag catataatct
    26821 cgcctccgat cagggcgagg ttatgctacg tgtgggttat aacttcgata tgggcgcggg
    26881 tattatgcta acaaatacct acacctttca gcgagaagat gaactcaaac atggctataa
    26941 tgaaattgaa ggttggtatc ctttatttaa acctaccgat aaattaacca tccagcccgg
    27001 tggcttaatt aatgacaaga gtatcggttc gggtggtgct gtttatctgg atgttaacta
    27061 caaatttact ccgtggttta acctgacggt gcgtaatcgc tataaccata ataattacag
    27121 ctctacagat ttgaacggcg aattggataa taacgacacc tatgaagttg gcacctactg
    27181 gaactttaaa ataacagaaa aattttccta tacatttgaa ccacattact tcatgcgagt
    27241 gaatgacttt aatagtagca acgggaaaga tcatcattgg gaaatcacta acaccttccg
    27301 ttaccgtatt aatgaacact ggcttcctta tttcgaattg cgctggttag accgcaacgt
    27361 cgagccgtac caccgcgagc aaaaccagat tcgtatcgga acgaagtatt tcttctgatg
    27421 taaaaattcc tgatgcgatg ctggcgcatc ttatcaggtc tacaaaagcc aaaaaaaaac
    27481 ctgccatcgc tggcaggttt tttatgacta aaaaacgaaa ttaatcgtct ttcggtgcgc
    27541 ggttggcgcg acgacgatcg ttttccgtca ggtgacgttt acgaatacgg atagaggtcg
    27601 gagtcacttc taccagttcg tcatcatcga tgaactccag agcttgttcc agagtcatgc
    27661 ggataggcgg aaccagaaca acggcttcgt cagtaccgga agcacgcatg ttggtcagtt
    27721 tcttaccggt caggcagttt acagtcaggt cgttagagcg gctgtgaata ccgataatct
    27781 gaccttcgta aacttcagca ccgtgaccga ggaacagctt accgcgatcc tgcagaccga
    27841 acagcgcgaa cgctaccgct ttaccctgac cgttagagat cagtacgccg ttctgacgct
    27901 ggcccacttc acccggacgt acgtcgtcgt agtggctgaa ggtggagtac agcagaccag
    27961 taccggaagt catggtcatg aactcagaac ggaagccaat cagaccacgg cttgggatca
    28021 cgtagtcgag acgtacgcgg cctttaccgt ctggattcat gtttttcagg tcgcctttac
    28081 gttcgcccag cgcctgcatt acagaaccct gatgctgttc ttcaacgtcc agagtcacgt
    28141 tttcatacgg ctcttgttta cgaccgtcga tttcacggaa gataactttc ggacgggata
    28201 ccgccagttc gaaaccttca cgacgcatgt tttcgataag aacagacagg tgcagttcgc
    28261 cacgaccaga aacgcggaac gcatcggcgt cttcggtttc ttctacgcgc agcgcaacgt
    28321 tgtgtaccag ttctttgttc agacgatcca ggatctgacg agacgttacg aacttacctt
    28381 ctttaccgca gaacggcgag gtgttaacgc agaagaacat agaaacggtc ggctcatcaa
    28441 cggagagtgc cggcagcgct tcaacgtttt gcgtgtcgca aacggtgtca gaaatgttca
    28501 gttcgccaag acccgtgatc gccacgatat cgccagcttc cgccagatcg gtttcgatac
    28561 gttccagacc gaggtggccc agcactttac cgactttcgc gttgcgggtt ttgccttcgc
    28621 tatcgatgat agtaacctgc tggttcggct tcactttacc gcgcttgatg cggccaatgc
    28681 cgataacgcc aacatagctg ttgtaatcga gctgagaaat ctgcatctgg aacggaccgt
    28741 caaggtcaac gtccggcgca ggaacgtggt caacaatcgc ctggtacagc ggggtcatgt
    28801 cttccgccat atcttcgtgg tccagacccg cgataccgtt cagcgcagaa gcgtaaacga
    28861 tcgggaagtc cagctgctcg tcggtcgcgt cgaggttaac gaacagatcg aatacctgat
    28921 ccacaaccca atcaggacgc gcgccagggc ggtcaacttt gttgataaca acaatcggct
    28981 tcaggccgta agcaaacgct tttttggtta cgaagcgcgt ttgcggcatc gggccgtcaa
    29041 atgcgtcaac caccagcagc acggagtcta ccatggacat tacacgttca acttcaccac
    29101 cgaagtcggc gtgccccggg gtatcaacga tgttgatacg gtaatcattc catttgatag
    29161 cggtgttttt cgcgaggatg gtaatcccac gctctttctc caaatcgttg gagtccatca
    29221 cgcgctcttg ggtttcggca cgagagtcga acgtaccgga ttgttggagc agcttgtcta
    29281 ccagggtggt tttaccatgg tctacgtgcg cgatgatggc gatattacgc aatttttcga
    29341 tcacaacttt gcctcaggca ttagaaatag cgcgttattg tacacggatt aatcgcacta
    29401 caaaacagga tcacaaacat cctccgcaaa caagtattgc agagtccctt tgtgatcgct
    29461 ttcacggagc ataaaaaggg ttatccaaag gtcattgcac caatatggtg cttaatgttc
    29521 ccattgaagc actatattgg tgcaacattc acatcatagt gcagcccttt tgcacgatgt
    29581 taagcatgat aacgcctttt aggggcaatt taaaagttgg cacagatttc gctttatctt
    29641 ttttacggcg acacggccca tttaattgca gatttcgtta ccacgacgac aatgaccaat
    29701 ccaggagagt taaagtatgt ccgctgaaca cgtactgacg atgctgaacg agcacgaagt
    29761 gaagtttgtt gatttgcgct tcaccgatac caaaggtaaa gaacagcacg tcactatccc
    29821 tgctcatcag gtgaatgccg aattcttcga agaaggcaaa atgtttgacg gctcctcgat
    29881 tggcggctgg aaaggcatta acgaatccga tatggtgctg atgccagacg catccaccgc
    29941 agtgattgac ccgttcttcg ccgattctac cctgattatc cgttgcgaca tccttgaacc
    30001 tggcaccctg caaggctatg accgtgaccc gcgctctatt gcgaagcgcg ctgaagatta
    30061 cctgcgttct actggcattg ccgacaccgt actgttcggg ccagaacctg aattcttcct
    30121 gttcgatgac atccgtttcg gatcgtctat ctccggttcc cacgttgcta tcgacgatat
    30181 cgaaggtgca tggaactcct ccacccaata tgaaggtggt aacaaaggtc accgtccggc
    30241 agtgaaaggc ggttacttcc cggttccgcc ggtagactca gctcaggata tccgttctga
    30301 aatgtgcctg gtgatggaac agatgggtct ggtggttgaa gctcatcacc acgaagtcgc
    30361 taccgcaggt cagaacgaag tggctacccg cttcaatacc atgaccaaaa aagctgacga
    30421 aattcagatc tacaaatatg tcgtgcacaa cgtggcgcat cgtttcggta agaccgcgac
    30481 ctttatgcca aaaccgatgt tcggtgataa cggttccggt atgcactgcc acatgtcgct
    30541 gtctaaaaac ggcgttaacc tgtttgctgg cgacaaatac gcaggtctgt ctgaacaagc
    30601 gctgtactac attggcggcg taatcaaaca cgcgaaagcg attaacgccc tggcaaaccc
    30661 gaccaccaac tcttacaagc gtctggtccc gggctacgaa gcaccggtaa tgctggctta
    30721 ctctgcgcgt aaccgttctg cgtctatccg tattccggtg gtttcttctc cgaaagcacg
    30781 tcgtatcgaa gtacgtttcc cggacccggc ggctaacccg tacctgtgct ttgctgccct
    30841 gctgatggcc ggtcttgatg gcatcaagaa caagatccat ccgggcgaag ccatggacaa
    30901 aaacctgtat gacctgccgc cagaagaagc gaaagagatc ccacaggttg caggctctct
    30961 ggaagaagca ctgaacgaac tggatctgga ccgcgagttc ctgaaagctg gtggcgtgtt
    31021 cacggatgaa gcgatcgatg cgtacatcgc tctgcgtcgc gaagaagatg accgcgtgcg
    31081 tatgactccg catccggtag agtttgagct gtactacagc gtctaatagt tgaagttgta
    31141 ctacccggcg caacaacgcc gggatttagt tgccgtggaa actttcagcc catctctgca
    31201 tgggcttttt tctccgtcaa ttctctgatg cttcgcgctt tttatccgta aaaagctata
    31261 atgcactaaa atggtgcaac ctgttcagga gactgcttta tggcaacagg cacgcagccc
    31321 gatgctgggc agatcctcaa ctcgctgatt aacagtattt tgttaatcga tgacaatctg
    31381 gcgatccatt acgccaaccc cgccgcgcaa caactgctcg cccaaagctc ccgcaaattg
    31441 tttggtacgc cgttaccgga actgttgagc tacttctcat taaatatcga gctgatgcaa
    31501 gaaagtctgg aggcggggca aggttttacc gataacgaag tgacgctggt catcgacggg
    31561 cgctcgcata tcctttctgt gacggctcag cgtatgccgg acggcatgat cctgctggag
    31621 atggcaccga tggataacca gcgccgctta agtcaggaac agctacagca cgcccagcag
    31681 gttgctgccc gtgatttagt gcgcggcctg gcacatgaga ttaaaaatcc gcttggcggt
    31741 ttacgtggcg cggcgcagct gctcagcaaa gcattacctg acccgtcact actcgaatat
    31801 accaaagtga ttatcgaaca ggcggaccgg ctgcgaaatc tggtcgaccg tctgttgggg
    31861 ccgcagctgc ccggtacgcg cattaccgaa agtattcaca aagtggctga acgcgtggtg
    31921 acgctggtgt cgatggaact gccgaacaac gtgcggttga ttcgggatta cgaccccagc
    31981 ctgccggaac tggcgcacga cccggatcaa attgaacagg tcttgctgaa tattgtgcgc
    32041 aatgcgctac aggcgctggg gccggagggt ggtgaaatca ttctgcgtac ccgcacagcg
    32101 tttcaactga ccttacacgg cgagcgctat cggctggcgg cgcggattga tgtggaagat
    32161 aacgggccgg gcattccgcc tcatttgcag gatacgctgt tttacccgat ggtcagcggc
    32221 cgcgaaggtg gcaccgggct tggcttatcc atcgcccgta atttgattga tcagcattca
    32281 ggcaaaattg aatttaccag ttggccaggt cataccgagt tctcggttta cctgcctatc
    32341 aggaaataaa ggtgacgttt atgcaacgag ggatagtctg ggtagtcgat gacgatagtt
    32401 ccatccgttg ggtgcttgaa cgtgcgctcg ctggagcggg tttaacctgt acgacatttg
    32461 agaacggcgc ggaagtactg gaggcgctgg cgagcaaaac gccggatgtg ctgctttcag
    32521 atatccgtat gccgggaatg gacgggctgg cgctgctcaa gcagattaaa cagcgccatc
    32581 cgatgcttcc ggtcatcatt atgaccgcac attccgatct ggatgctgcc gtcagcgcct
    32641 atcaacaagg ggcatttgat tatctgccca aaccgtttga tatcgacgaa gccgtggcgc
    32701 tggttgagcg cgccatcagt cattaccagg aacagcagca gccgcgtaat gttcagctta
    32761 acggcccaac gaccgatatc atcggtgagg cgccagccat gcaggacgtg ttccggatta
    32821 tcggtcggct ttcgcgttct tctattagcg tgctgattaa cggcgagtcc ggcaccggta
    32881 aagaactggt cgctcatgcc ctgcatcgcc acagtccgcg agccaaagcg ccgtttatcg
    32941 cgctcaatat ggcggctatc ccgaaggatt tgatcgaatc cgaactgttt ggtcacgaga
    33001 aaggcgcatt taccggcgcg aataccattc gtcaggggcg ttttgaacag gctgatggcg
    33061 gtacattatt tctcgatgaa attggcgata tgccgctaga tgtgcagacg cgtttgctgc
    33121 gcgtgctggc agacggtcag ttttatcgcg ttggcggcta tgcgccggtg aaagtagatg
    33181 tgcggattat cgctgccact caccagaatc ttgaacagcg ggtgcaggaa ggcaagttcc
    33241 gtgaggatct gttccaccgc ctgaacgtca tccgcgttca tctgccgccg ctgcgcgaac
    33301 gtcgggaaga tattccccgt ctggcgcgcc attttttaca ggttgccgcg cgagagctgg
    33361 gcgtagaagc gaagttgctg catccggaaa ccgaagccgc gctgacgcgt ctgacgtggc
    33421 caggcaacgt gcgccagctg gaaaacacct gccgctggct aacggtaatg gctgccggac
    33481 aggaagtgtt gattcaggat ttgcctggcg aactgtttga atccaccgta gcagaaagcc
    33541 cttcgcacat gcagccggac agttgggcaa cgctgttagc acagtgggca gacagagcgc
    33601 tgcgttccgg tcatcaaaac ctgctttccg aagcacagcc agagctggaa cggacgttac
    33661 tgaccaccgc gctgcgacat acgcaggggc ataaacagga agcggcgcgg ctacttggct
    33721 ggggccgcaa caccctgacg cgtaagttaa aagagctggg gatggagtga ttcacggctt
    33781 gtgtgtaaag attgattatt gagcgcaaat tgctggtatt ttgcgcttta ctgttccgat
    33841 aagttcagta tgatcttgcc cggaaaacgg ggagagtcat tatgctggaa tcaataatta
    33901 atctggtatc gagtggcgca gttgacagcc acacaccgca aactgctgtt gctgccgtgc
    33961 tgtgtgccgc gatgataggg ctgtttagct gaagctcgtt gtttacgccg gatgcggaga
    34021 aagtcgtagg cctgataaga cgcaaccagc gtcgcatcag gcgttgattg ccggatgcgg
    34081 cgtaaacgcc ttatccggcc tacaaaagca ataatttcaa cagattttgc aggcctgata
    34141 agcgtagcgc atcaggcaat cttgttctca gcacattgat ggcgctttcg ttttctactt
    34201 tgtaaacgaa gcgccattca caatttaaat cacccgagag aactgctgca tccgcgcttt
    34261 ctggcgcaga taggtatcaa agcacatgca aatgttgcgg atcagcaagc gacctttcgc
    34321 cgtcacctgt attcccttct catccacatc caccagccca tcttttgcta acggggcgag
    34381 caacttgaga tcttccgcaa agtaatcagc gaagagcaaa tcccactgtt gctcaatagg
    34441 ggcgtaatcc agacggaagt tgcagatgag cgacttaatc acatcgcggc gaatacagtc
    34501 atcacgcgtt agcgcaatac cacgccacag cgcattgcct tgttcatcca cttgctgata
    34561 gtactgcttc aactctttct ggttctgcgc gtagcagtcg ccaatcatgc tgatggcgga
    34621 aacgcccatc cccagcagat cggtatcgcc ctgagtggtg tagccctgga agttacgatg
    34681 cagcacgcct tcacgctggg ccaccgccag ctcgtcatcc ggacgggcaa agtgatccat
    34741 accgataaac tgatagcccg attgtgtcag gaaggcgatg gtttcctgca ggatatcgag
    34801 tttttgctgc ggactcggca gttcagcgtc tttgattttg cgctgagcag caaaaatggt
    34861 cggcaaatgc gcgtagttaa agacgctcag acgatcgggg ttcagctccg ccacgcgttt
    34921 cagggtaaag gcgaaactct ccggcgtctg tttcggcagg ccgtaaatca ggtcgatgtt
    34981 ggtggaggta aagccaatct cacgcgcatg gttaagcagt gcaaagatga actcttcatc
    35041 ctgctcgcgg ttaaccagac gttgcacttc tttgttgaag tcctgcacgc ccatgctcag
    35101 ccgattaaag ccttcggcgc gtaaatgatc gagtacatcc agttcgattt cccgcggatc
    35161 gacttcgatc gagatctccg catcggcatt gaactggaag ttttcgcgca gcagcttcat
    35221 cagacggctg atttgcgctt tattcagata cgtcggcgtt ccgccgcccc agtgcaattg
    35281 gctgacgtga cgcccggcga acagcggtgc acgatggacg atttcttgct ccagcgcgtc
    35341 cagatactga tcggccttgt gctgctggcg agtaacaatc ttattgcaac cgcagaagta
    35401 acaaagctta tggcagaacg gaatatgtac gtagagagat aatggacgct caggatagcg
    35461 cgccacggct tgtaaaaacg cctgttcgcc gaagtcttct gaaaactcca gcgcggtcgg
    35521 gtacgaggtg tatcgtggcc cggaatagtt atatttctgg atcagggcca gatcccagtc
    35581 gatttgctgt acagacatgc tcactccttc cgatggcgtc tctggcggct acggcgtacc
    35641 ggcggatgcc cgtttcgagt catcagccgg ttgcgcagcc acttttgtcg ccgcgacaac
    35701 cgctgtagtt taacgaataa ccacaccaga taacatataa ccggaagggt tatcagcaga
    35761 acgggaaggc ccacggcggc aatccgttag ttgccccgca gcagacgcat catgtcttct
    35821 tgcttctcgt cttcttcctc ttcttcgtca tcgtcataag agaggccgag tttctgcatc
    35881 aactcatcaa tacgatccag tttggcatcc acccaggatt gctcttcggc gctcagggtt
    35941 tcgcctgctt ccagacgttc cagcagcgca tccagacgct catccgtttc cagtaactcc
    36001 aactccgcct gcggtgaaag cataggtttc tcactcttcg gtttgtgctg tttggtgact
    36061 ttttcagtca cgcccaatgg aataggggtt ttactgccaa tacgtggatc ttttggtgcg
    36121 ttctgacctt tgctgcctga cgtggtgtta ccgcccgctg cgcggctgcc cggcacatga
    36181 ccacgacgtt ttttctgacg cttacggtca cgagcttcct gatccagctc ctcgcgtgtt
    36241 tttcgacgcg cttttgcatg acctttgctg cgtgagtttg aagatgatgg tttcataatg
    36301 gttcgtcttt tagccgtttt attcagtata gatttgcggc ggaatctaac agaaagcaag
    36361 caaagaaaaa aggcgacaga ttactctgtc gccttttttc ctgactcata acccttaacg
    36421 ggtcttacaa tccttgcagg ccgccaacac accctgtttt tccattagcc ggaacaatcc
    36481 gtgttgattc catttccatt taattacgtc tccggacgtt tatcttcctg acaatcctct
    36541 gtcttcgcct cctggcgctc ctgaccctca tcctgagtca ttgttcctgt tagcgtcctc
    36601 gctctatggg tgctacttta cgcctttgct ttaaataaac aatcaaccgc catcagtatt
    36661 tgcacatttt cagagtttta tataaaagca attcactgat ttatatagct tatttgacat
    36721 acgtcgcaga attttctctg ctatttcgca taaagactat cggcaatgtc tcacaaagca
    36781 tatggctttt acttacaacg ctcacgccga aaaatccgcc ttttattatg gttcaggtat
    36841 aatcgccgca aagctttgac tgatggagac gaccgctttg actaatttga attatcaaca
    36901 gacgcatttt gtgatgagtg cgcctgatat tcgccaccta ccttccgata ccggaattga
    36961 agtggctttt gcaggccgtt ccaacgcagg taaatccagc gcgctgaaca cgctgactaa
    37021 ccagaaaagc ctggctcgta cctcaaaaac cccggggcgc acccagctta tcaacctgtt
    37081 tgaagtggct gacggcaagc gtctggttga cttgcctggg tacggttatg cggaagtccc
    37141 ggaagagatg aagcgcaaat ggcagcgcgc gctcggcgag tacctcgaaa aacgtcagag
    37201 cctgcaaggt ctggtggtgc taatggatat tcgccatccg ctgaaagatt tggatcagca
    37261 gatgattgag tgggcggtag acagcaatat cgccgtgctg gtactgctga ccaaagcgga
    37321 caaactggca agcggcgcac gtaaagcgca attgaatatg gtgcgtgaag cggtgctggc
    37381 gtttaacggt gatgtgcagg ttgaaacgtt ttcttcgctg aagaaacaag gcgtggacaa
    37441 gctgcggcag aaactggata cctggtttag cgagatgcag cctgtagaag aaacgcagga
    37501 cggcgaataa ttttcttgcc ttaatgcttg tgccggatgt ggcgtatccg gcccgtaaat
    37561 tcaatagatt acaattctac cgttggcctg ataaacatgg cgtatcaggc attacggatc
    37621 ttttctttcg cccaataaaa aacgccccag tcattactga ctggggcggc taaaatattc
    37681 agccaaatcc gattacgtga agtaaaaggt ctgaaagata gaacatctta cctctgtacc
    37741 ctacgcgact aactttactc ttttttgttc agttcagaaa gcattttttg taattttttt
    37801 tcagttgttg cataggaatt tctaatagcc atcacaaaac gtgacagctt atgttgctta
    37861 cttacgaaaa aagggcatgt tcaggcgaat cttagtgcgc ctgatcccag ttttcgccac
    37921 tccccacttc caccagtaac ggcacatcca gacgggtaca gttttccatc agttgatgaa
    37981 tctgcttcgc gacggcatcg acatcatctt tatggacttc aaataccagt tcatcgtgta
    38041 cctgcatgat catacgcaca cgcggttgct cagcctgtag ccacgcatca acggcaatca
    38101 tcgcccgttt gataatgtcg gcggcggttc cctgcattgg cgcgttaatg gctgcacgtt
    38161 cagccgctgc acgacgagca ccattgctgg atttgatatc cggcagatac agacggcgtc
    38221 cgtccagcgt ttcaacgtag ccctgctctt tcgcctgagc acgggtgcgt tccatatact
    38281 ccagcacgcc agggtagcgt tcgaagtaaa ggtccatgta cttctgcgct tctttacgcg
    38341 gaatgttcaa ttgccgcgcc agaccgaaag cactcatgcc ataaatcaga ccaaagttga
    38401 tcgctttcgc gctacgacgt tgctcgctgg tgacggtttc cagtggcaaa ccaaacactt
    38461 ctgccgccgt tgcacggtgg atatcttttc cttcggcgaa tgcggtcagc aagcctttgt
    38521 cacgcgagag atgcgccata atgcgcagtt caatctgcga gtagtccgct gagacaatca
    38581 cataatcctc tggcgcaata aacgcctggc ggatacggcg accttcttcg ttacgcaccg
    38641 gaatgttttg caggttagga tcggttgacg ataaacgtcc cgttgcggtt actgcctggt
    38701 gataagaggt atgtacacgc ccggttttcg ggttgatcat caacggcagc ttgtcggtgt
    38761 aggtcgattt cagcttcgcc agaccacgat actccagaat cacttttggc aacggatagt
    38821 ccagcgccag ttcttccagt acctcttccg acgttgacgg cgcgccaccc ggcgttttct
    38881 tcagcggttt aatgccctgt ttttcaaaca gaatggtttg taactgcttg gtggaagaaa
    38941 ggttaaattc ttcacctgca atttcatgcg ctttcttttc cagctcagcc agacgcaagg
    39001 tgagctcttc agagtgattg tgcagcactt tcggatcgat cttcacaccg ttacgttcaa
    39061 tgcgtgaaag caccggcacc agcggcattt cgatattctc gaagacgttc aacggccctt
    39121 tgtgtttttg cagatccggc cacattttca gatgcaactg caaggtgaca tctgcatctt
    39181 cggcggcgta acgtccggct tcttcgaggg caatctggtt aaaggtcagt tgatttttgc
    39241 ctttacccgc aatctcttca aaagtgatgg ttttgtgctt caaccaacgt tccgcgaggc
    39301 tgtccatatc gtgacgtccg gcaacgctat tgagaatgta ggactccagc atggtatcaa
    39361 acgcaatccc acgtagttca atgccgtagt tcgccagaat accgcgatcg tatttcaggt
    39421 tttgcccgac cttcagcgcc ttttcatctt ccagcagcgg ttttagcagc tcgagtgcac
    39481 gctcgcgaga gatttgatcg ggcgcatcaa gataatcatg agcaaccgga atatatgccg
    39541 ctacgcctgg ctcgatagca aaagaaagcc cgaccaggtt agcagagatg ttatcaaggc
    39601 tgtcggtttc ggtatcaaat gcaaataccg gcgctttttc cagcttcgca atccacgctt
    39661 tcagtgtttc ttcatcaagg atggtgacgt agttgtcata agaaatcacc gttgccgtca
    39721 cttctggcgc ttcgtctgca acactggttt cctgcggcct ggctgcgggt ttcgctcctt
    39781 tagcctgtag ccattttccc gcttcgacat cagcagtcca gcgtttgaac tcatactttt
    39841 tgaacagccc taacaactct tccgccgccg gtggctgcac ttccagttgt tcacaggtca
    39901 gctccagttc aacgtcggtt ttaatcgtcg ccagctggta tgagagataa gcaacttctt
    39961 tgttttgctc gagcttggct gccattgttt tcgcgccacg gaagctcaac ccagcaattt
    40021 tttctggctc ggcatacagc gtatccagtc cgccaagacc ttgcagcaat gcctgcgcgg
    40081 ttttttcacc gacgcccggt acgccaggaa tgttatcaga ggagtcaccc atcagcgcca
    40141 ggaaatcgat gatcagttct ggcggcacgc cgtacttatt caccacctct tccggtccga
    40201 ggatggtatt cgtcatggta ttgataagcg taatatttgg cgtcaccagc tgcgccatat
    40261 ctttatcgcc agtgctgatc agcaccggac gcccggcttt ttcggcttcg cgcgccagag
    40321 taccgataac gtcgtccgct tctacgccag aaaccgccag cagcggcagt cccatcgctt
    40381 taaccatcgc gtgcagaggt tcgatttgtg ctcgcagatc gtccggcatt ggcgggcgat
    40441 gtgatttgta atgttcaaac agttcatcac gaaaggtttt tcccttggcg tcaaagacca
    40501 ccgctgcatg cgtcggttta tattgcatga tcagactgcg cagcatgttg aggacaccat
    40561 acatcgcacc ggtcggctcg cctgcgctgt tagtcagcgg gggaaacgcg tgatatgcgc
    40621 gataaagata agatgaacca tctacaagga taagtggatt ttgggggatc tgaaccataa
    40681 tgtccgtgcc tgtttatcag attatgggta aaggatgcca cagacaggat gaaaacatga
    40741 gtttttcacc gcttttcgtg aaaatctttt gtagatcttc tggatcgctc gcaaatcgtc
    40801 atgtggataa ctttgtgcat aaattattta catcgctttt aatttattat c
//
LOCUS       DADSYM010000019        40640 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2415
            SAMN08039579-rid11723173.denovo.019, whole genome shotgun sequence.
ACCESSION   DADSYM010000019 DADSYM010000000
VERSION     DADSYM010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039579
            Sequence Read Archive: SRR6362533
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 40640)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40640)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980162.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 03:39:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,484
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 4,347
            CDSs (with protein)               :: 4,347
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 43 of 85
            Pseudo Genes (incomplete)         :: 37 of 85
            Pseudo Genes (internal stop)      :: 19 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40640
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039579-rid11723173.denovo.019"
                     /strain="LGE2415"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8302 N 95.9628 W"
                     /collection_date="2015-08-24"
     gene            427..1494
                     /locus_tag="J1Y83_000870"
     CDS             427..1494
                     /locus_tag="J1Y83_000870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HBA7629046.1"
                     /translation="MDKSKRHLAWWVVGALAVAAVVAWWLLRPAGVPEGFAVSNGRIE
                     ATEVDIASKIAGRIDTILVKEGQFVREGEVLAKMDTRVLQEQRLEAIAQIKEAQSAVA
                     AAQALLEQRQSETRAAQSLVNQRQAELDSVAKRHTRSRSLAQRGAISAQQLDDDRAAA
                     ESARAALESAKAQVSASKAAIEAARTNIIQAQTRVEAAQATERRIAADIDDSELKAPR
                     DGRVQYRVAEPGEVLAAGGRVLNMVDLSDVYMTFFLPTEQAGTLKLGGEARLILDAAP
                     DLRIPATISFVASVAQFTPKTVETSDERLKLMFRVKARIPPELLQQHLEYVKTGLPGV
                     AWVRVNEELPWPDDLVVRLPQ"
     gene            1491..4226
                     /gene="rbbA"
                     /locus_tag="J1Y83_000871"
     CDS             1491..4226
                     /gene="rbbA"
                     /locus_tag="J1Y83_000871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated ATPase/putative transporter
                     RbbA"
                     /protein_id="HBA7629047.1"
                     /translation="MTHLELVPVPPVAQLAGVSQHYGKTVALNNITLDIPARCMVGLI
                     GPDGVGKSSLLSLISGARVIEQGSVMVLGGDMRDPKHRRDVCPRIAWMPQGLGKNLYH
                     TLSVYENVDFFARLFGHDKAEREVRINELLTSTGLAPFRDRPAGKLSGGMKQKLGLCC
                     ALIHDPELLILDEPTTGVDPLSRAQFWDLIDSIRQRQSNMSVLVATAYMEEAERFDWL
                     VAMNAGEVLATGSAEELRQQTQSATLEEAFINLLPQAQRQAHQAVVIPPYQPENAEIA
                     IEARDLTMRFGSFVAVDHVNFRIPRGEIFGFLGSNGCGKSTTMKMLTGLLPASEGEAW
                     LFGQPVDPKDIDTRRRVGYMSQAFSLYNELTVRQNLELHARLFHIPEAEIPARVAEMS
                     ERFKLNDVEDVLPESLPLGIRQRLSLAVAVIHRPEMLILDEPTSGVDPVARDMFWQLM
                     VDLSRQDKVTIFISTHFMNEAERCDRISLMHAGKVLASGTPPELVEKRGAASLEEAFI
                     AYLQEAAGQSNEAEAPPVVHDTTHAPRQGFSLRRLFSYSRREALELRRDPVRSTLALM
                     GTVILMLIMGYGISMDVENLRFAVLDRDQTVSSQAWTLNLSGSRYFIEQPPLTSYDEL
                     DRRMRAGDITVAIEIPPNFGRDIARGTPVELGVWIDGAMPSRAETVKGYVQAMHQSWL
                     QDVASRQSTPSSQSGLMNIETRYRYNPDVKSLPAIVPAVIPLLLMMIPSMLSALSVVR
                     EKELGSIINLYVTPTTRSEFLLGKQLPYIALGMLNFFLLCALSVFVFGVPHKGSFLTL
                     TLAALLYIIIATGMGLLISTFMKSQIAAIFGTAIITLIPATQFSGMIDPVASLEGPGR
                     WIGEVYPTSHFLTIARGTFSKALDLTDLWQLFIPLLIAIPLVMGLSILLLKKQEG"
     gene            4226..5350
                     /locus_tag="J1Y83_000872"
     CDS             4226..5350
                     /locus_tag="J1Y83_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 transporter permease"
                     /protein_id="HBA7629048.1"
                     /translation="MRHLRNIFNLGIKELRSLLGDKAMLTLIVFSFTVSVYSSATVTP
                     GSLNLAPIAIADMDQSQLSNRIVNSFYRPWFLPPEMITADEMDAGLDAGRYTFAINIP
                     PNFQRDVLAGRQPDIQVNVDATRMSQAFTGNGYIQNIINGEVNSFVARYRDNSEPLVS
                     LETRMRFNPNLDPAWFGGVMAIINNITMLAIVLTGSALIREREHGTVEHLLVMPITPF
                     EIMMAKVWSMGLVVLVVSGLSLVLMVKGVLGVPIEGSIPLFMLGVALSLFATTSIGIF
                     MGTIARSMPQLGLLVILVLLPLQMLSGGSTPRESMPQMVQDIMLTMPTTHFVSLAQAI
                     LYRGAGFEIVWPQFLTLIAIGGAFFTIALLRFRKTIGTMA"
     gene            complement(5359..5760)
                     /gene="nikR"
                     /locus_tag="J1Y83_000873"
     CDS             complement(5359..5760)
                     /gene="nikR"
                     /locus_tag="J1Y83_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-responsive transcriptional regulator
                     NikR"
                     /protein_id="HBA7629049.1"
                     /translation="MQRVTITLDDDLLETLDSLSQRRGYNNRSEAIRDILRSALAQEA
                     TQQHGTQGFAVLSYVYEHEKRDLASRIVSTQHHHHDLSVATLHVHINHDDCLEIAVLK
                     GDMGDVQHFADDVIAQRGVRHGHLQCLPKED"
     gene            complement(5766..6572)
                     /gene="nikE"
                     /locus_tag="J1Y83_000874"
     CDS             complement(5766..6572)
                     /gene="nikE"
                     /locus_tag="J1Y83_000874"
                     /EC_number="7.2.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel import ATP-binding protein NikE"
                     /protein_id="HBA7629050.1"
                     /translation="MTLLNVSDLSHHYAHGGFSGKHQHQAVLNNVSLALKSGETVALL
                     GRSGCGKSTLARLLVGLESPSQGNISWRGEPLAKLNRAQRKAFRRDIQMVFQDSISAV
                     NPRKTVREILREPMRHLLSLKKAEQLARASEMLKAVDLDDSVLDKRPPQLSGGQLQRV
                     CLARALAVEPKLLILDEAVSNLDLVLQAGVIRLLKKLQQQFGTACLFITHDLRLVERF
                     CQRVMVMDNGQIVETQVVGDKLTFSSDAGRVLQNAVLPAFPVRRRTTEKV"
     gene            complement(6569..7333)
                     /gene="nikD"
                     /locus_tag="J1Y83_000875"
     CDS             complement(6569..7333)
                     /gene="nikD"
                     /locus_tag="J1Y83_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel import ATP-binding protein NikD"
                     /protein_id="HBA7629051.1"
                     /translation="MPQQIELRNIALQAAQPLVHGVSLTLQRGRVLALVGGSGSGKSL
                     TCAATLGILPAGVRQTAGEILADGKPVSPCALRGIKIATIMQNPRSAFNPLHTMHTHA
                     RETCLALGKPADGATLTAAIEAVGLENAARVLKLYPFEMSGGMLQRMMIAMAVLCESP
                     FIIADEPTTDLDVVAQARILDLLESIMQKQAPGMLLVTHDMGVVARLADDVAVMSHGK
                     IVEQGDVETLFNAPKHAVTRSLVSAHLALYGMELAS"
     gene            complement(7333..8166)
                     /gene="nikC"
                     /locus_tag="J1Y83_000876"
     CDS             complement(7333..8166)
                     /gene="nikC"
                     /locus_tag="J1Y83_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter permease subunit NikC"
                     /protein_id="HBA7629052.1"
                     /translation="MNFFLSSRWSVRLALIIIALLALIALTSQWWLPYDPQAIDLPSR
                     LLSPDAQHWLGTDHLGRDIFSRLMAATRVSLGSVMACLLLVLTLGLIIGGSAGLIGGR
                     VDQATMRVADMFMTFPTSILSFFMVGVLGTGLTNVIIAIALSHWAWYARMVRSLVISL
                     RQREFVLASRLSGAGHVRVFIDHLAGAVIPSLLVLATLDIGHMMLHVAGMSFLGLGVT
                     APTAEWGVMINDARQYIWTQPLQMFWPGLALFISVMAFNLVGDALRDHLDPHLVTEHA
                     H"
     gene            complement(8163..9107)
                     /gene="nikB"
                     /locus_tag="J1Y83_000877"
     CDS             complement(8163..9107)
                     /gene="nikB"
                     /locus_tag="J1Y83_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter permease subunit NikB"
                     /protein_id="HBA7629053.1"
                     /translation="MLRYVLRRFLLLIPMVLAASVIIFLMLRLGTGDPALDYLRLSNL
                     PPTPEMLASTRTMLGLDQPLYVQYGTWLWKALHLDFGISFASQRPVLDDMLNFLPATL
                     ELAGAALVLILLTSVPLGIWAARHRDRLPDFAVRFIAFLGVSMPNFWLAFLLVMAFSV
                     YLQWLPAMGYGGWQHIILPAVSIAFMSLAINARLLRASMLDVAGQRHVTWARLRGLSD
                     KQTERRHILRNASLPMITAVGMHIGELIGGTMIIENIFAWPGVGRYAVSAIFNRDYPV
                     IQCFTLMMVVVFVVCNLIVDLLNAALDPRIRRHEGAHA"
     gene            complement(9107..10681)
                     /gene="nikA"
                     /locus_tag="J1Y83_000878"
     CDS             complement(9107..10681)
                     /gene="nikA"
                     /locus_tag="J1Y83_000878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter, nickel/metallophore
                     periplasmic binding protein"
                     /protein_id="HBA7629054.1"
                     /translation="MFSTLRRTLFALLACASFIVHAAAPDEITTAWPVNVGPLNPHLY
                     TPNQMFAQSMVYEPLVKYQADGSVIPWLAKSWTHSEDGKTWIFTLRDDVKFSNGEPFD
                     AEAAAENFRAVLDNRQRHAWLELANQIVDVKALSKNELQITLKSAYYPFLQELALPRP
                     FRFIAPSQFKNHETMNGIKAPIGTGPWVLQESKLNQYDVFVRNENYWGEKPAIKKITF
                     NVIPDPTTRAVAFETGDIDLLYGNEGLLPLDTFARFSQNPAYHTQLSQPIETVMLALN
                     TAKAPTNELAVREALNYAVNKKSLIDNALYGTQQVADTLFAPSVPYANLGLKPRQYDP
                     QKAKALLEKAGWTLPAGKDIREKNGQPLRIELSFIGTDALSKSMAEIIQADMRQIGAD
                     VSLIGEEESSIYARQRDGRFGMIFHRTWGAPYDPHAFLSSMRVPSHADFQAQQGLADK
                     PLIDKEIGEVLATHDETQRQALYRDILTRLHDEAVYLPISYISMMVVSKPELGNIPYA
                     PIATEIPFEQIKPVKP"
     gene            complement(10792..11379)
                     /gene="acpT"
                     /locus_tag="J1Y83_000879"
     CDS             complement(10792..11379)
                     /gene="acpT"
                     /locus_tag="J1Y83_000879"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase AcpT"
                     /protein_id="HBA7629055.1"
                     /translation="MYRIVLGKVSTLSAAPLPPGLREQAPQGPRRERWLAGRALLSHT
                     LSPLPEIIYGEQGKPAFAPETPLWFNLSHSGDDIALLLSDEGEVGCDIEVIRPRANWR
                     WLANAVFSLGEHAEMDAVHPDQQLEMFWRIWTRKEAIVKQRGGSAWQIVSVDSTYNSS
                     LSVSHCQLENLSLAICTPTPFTLTADSVQWIDSVN"
     gene            complement(11434..12483)
                     /locus_tag="J1Y83_000880"
     CDS             complement(11434..12483)
                     /locus_tag="J1Y83_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HBA7629056.1"
                     /translation="METPQPDKTGMHILLKLASLVVILAGIHAAADIIVQLLLALFFA
                     IVLNPLVTWFIRRGVQRPVAITIVVVVMLIALTALVGVLAASFNEFISMLPKFNKELT
                     RKLFKLQEMLPFLNLHMSPERILQRMDSEKVVTFTTALMTGLSGAMASVLLLVMTVVF
                     MLFEVRHVPYKMRFALNNPQIHIAGLHRALKGVSHYLALKTLLSLWTGIIVWLGLELM
                     GVQFALMWAVLAFLLNYVPNIGAVISAVPPMIQVLLFNGIYECILVGALFLVVHMVIG
                     NILEPRMMGHRLGMSTMVVFLSLLIWGWLLGPVGMLLSVPLTSVCKIWMETTKGGSKL
                     AILLGPGRPKSRLPG"
     gene            12582..13832
                     /locus_tag="J1Y83_000881"
     CDS             12582..13832
                     /locus_tag="J1Y83_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312349.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBA7629057.1"
                     /translation="MKHCCKNVVILMPEPVAEPALNGLRLNLRIVSIVMFNFASYLTI
                     GLPLAVLPGYVHDVMGFSAFWAGLVISLQYFATLLSRPHAGRYADLLGPKKIVVFGLC
                     GCFLSGLGYLTAGLTASLPVISLLLLCLGRVILGIGQSFAGTGSTLWGVGVVGSLHIG
                     RVISWNGIVTYGAMAMGAPLGVVFYHWGGLQALALIIMGVALVAILLAIPRPTVKASK
                     GKPLPFRAVLGRVWLYGMALALASAGFGVIATFITLFYDAKGWDGAAFALTLFSCAFV
                     GTRLLFPNGINRIGGLNVAMICFSVEIIGLLLVGVATMPWMAKIGVLLAGAGFSLVFP
                     ALGVVAVKAVPQQNQGAALATYTVFMDLSLGVTGPLAGLVMSWAGVPVIYLAAAGLVA
                     IALLLTWRLKKRPPVEIPEAASSS"
     gene            complement(13836..14393)
                     /gene="dcrB"
                     /locus_tag="J1Y83_000882"
     CDS             complement(13836..14393)
                     /gene="dcrB"
                     /locus_tag="J1Y83_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417929.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage sensitivity protein DcrB"
                     /protein_id="HBA7629058.1"
                     /translation="MRNLVKYVGIGLLVMGLAACDDKDTNATAQGSVAESNATGNPVN
                     LLDGKLSFSLPADMTDQSGKLGTQANNMHVWSDATGQKAVIVIMGDDPKEDLAVLAKR
                     LEDQQRSRDPQLQVVTNKAIELKGHKMQQLDSIISAKGQTAYSSVILGNVGNQLLTMQ
                     ITLPADNQQKAQTTAENIINTLVIQ"
     gene            complement(14466..15131)
                     /locus_tag="J1Y83_000883"
     CDS             complement(14466..15131)
                     /locus_tag="J1Y83_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine/7-aminomethyl-7-
                     deazaguanine transporter"
                     /protein_id="HBA7629059.1"
                     /translation="MNVFSQTQRYKALFWLSLFHLLVITSSNYLVQLPVSIFGFHTTW
                     GAFSFPFIFLATDLTVRIFGAPLARRIIFAVMIPALLISYVISSLFYMGSWQGFGALA
                     HFNLFVARIATASFMAYALGQILDVHVFNRLRQSRHWWLAPTASTLFGNVSDTLAFFF
                     IAFWRSPDAFMAEHWMEIALVDYCFKVLISIVFFLPMYGVLLNMLLKRLADKSEINAL
                     QAS"
     gene            15352..15597
                     /gene="tusA"
                     /locus_tag="J1Y83_000884"
     CDS             15352..15597
                     /gene="tusA"
                     /locus_tag="J1Y83_000884"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA"
                     /protein_id="HBA7629060.1"
                     /translation="MTDLFSSPDHTLDALGLRCPEPVMMVRKTVRNMQPGETLLIIAD
                     DPATTRDIPGFCTFMEHELVAKETDGLPYRYLIRKGG"
     gene            complement(15866..18064)
                     /gene="zntA"
                     /locus_tag="J1Y83_000885"
     CDS             complement(15866..18064)
                     /gene="zntA"
                     /locus_tag="J1Y83_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn(II)/Cd(II)/Pb(II) translocating P-type ATPase
                     ZntA"
                     /protein_id="HBA7629061.1"
                     /translation="MSTPDNHGKKAPQFAAFKPLTTVQNTNDCCCDGACSSTPTLSEN
                     VSGTRYSWKVSGMDCAACARKVENAVRQLAGVNQVQVLFATEKLVVDADNDIRAQVES
                     AVQKAGYSLRDEQASDEPQESRLKENLPLITLIVMMAISWGLEQFNHPFGQLAFIATT
                     LVGLYPIARQALRLIKSGSYFAIETLMSVAAIGALFIGATAEAAMVLLLFLIGERLEG
                     WAASRARQGVSALMALKPETATRLRNGEREEVAINSLRPGDVIEVAAGGRLPADGKLL
                     SPFASFDESALTGESIPVERATGDKVPAGTTSVDRLVTLEVLSEPGASAIDRILKLIE
                     EAEERRAPIERFIDRFSRIYTPAIMAVALLVTLVPPLLFAASWQEWIYKGLTLLLIGC
                     PCALVISTPAAITSGLAAAARRGALIKGGAALEQLGRVTLVAFDKTGTLTVGKPRVTA
                     IHPATGISESELLTLAAAVEQGATHPLAQAIVREAQVAELAIPTAQSQRALVGSGIEA
                     QVNGERVLICAAGKHPADAFAGLINELESAGQTVVLVVRNDDVLGVIALQDTLRADAA
                     TAISELNALGVKGVILTGDNPRAAAAIAGELGLEFKAGLLPEDKVKAVTELNQHAPLA
                     MVGDGINDAPAMKAAAIGIAMGSGTDVALETADAALTHNHLRGLVQMIELARATHANI
                     RQNITIALGLKGVFLVTTLLGMTGLWLAVLADTGATVLVTANALRLLRRK"
     gene            complement(18138..18764)
                     /locus_tag="J1Y83_000886"
     CDS             complement(18138..18764)
                     /locus_tag="J1Y83_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysoplasmalogenase"
                     /protein_id="HBA7629062.1"
                     /translation="MLWSFIAVCLSAWLSVDASYRGPTWQRWVFKPLTLLLLLLLAWQ
                     APMFDAISYLVLAGLCASLLGDALTLLPRQRLMYAIGAFFLSHLLYTIYFASQMTLSF
                     FWPLPLVLLVLGALLLAIIWTRLEEYRWPICTFIGMTLVMVWLAGELWFFRPTAPALS
                     AFVGASLLFISNFVWLGSHYRRRFRADNAIAAACYFAGHFLIVRSLYL"
     gene            18905..19264
                     /locus_tag="J1Y83_000887"
     CDS             18905..19264
                     /locus_tag="J1Y83_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2500 domain-containing protein"
                     /protein_id="HBA7629063.1"
                     /translation="MSKPPLFFIVIIGLIIVAASFRFMQQRREKADNDMAPLQQKLVV
                     VSNKREKPLNDRRSRQQEVTPAGTSMRYEASFKPQSGGMEQTFRLDAQQYHALTVGDK
                     GTLSYKGTRFVSFVGEQ"
     gene            complement(19267..19536)
                     /locus_tag="J1Y83_000888"
     CDS             complement(19267..19536)
                     /locus_tag="J1Y83_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1145 family protein"
                     /protein_id="HBA7629064.1"
                     /translation="MLINIGRLLMLCVWGFLILNLVHPFPRPLNIFVNVALIFTVLMH
                     GMQLALLKSTLPKDGPQMTTAEKVRIFLFGVFELLVWQKKFKVKK"
     gene            complement(19526..20122)
                     /gene="rsmD"
                     /locus_tag="J1Y83_000889"
     CDS             complement(19526..20122)
                     /gene="rsmD"
                     /locus_tag="J1Y83_000889"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase"
                     /protein_id="HBA7629065.1"
                     /translation="MKKPNHSGSGQIRIIGGQWRGRKLPVPDSPGLRPTTDRVRETLF
                     NWLAPVIVDAQCLDCFAGSGALGLEALSRYAAGATLIEMDRAVSQQLIKNLATLKAGN
                     ARVVNSNAMSFLAQKGTPHNIVFVDPPFRRGLLEETINLLEDNGWLADEALIYVESEV
                     EKGLPTVPANWSLHREKVAGQVAYRLYQREAQGESDAD"
     gene            20272..21696
                     /gene="ftsY"
                     /locus_tag="J1Y83_000890"
     CDS             20272..21696
                     /gene="ftsY"
                     /locus_tag="J1Y83_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HBA7629066.1"
                     /translation="MAKEKKRGFFSWLGFGQKEQTPEKETEVQNEQPVVEEIVQAQEP
                     VKASEHAVEEQPQAHTEAEAETFAADVVEVTEQVAESEKAQPEAEVVAQPEPVVEETP
                     EPVAIEREELPLSEDVNAEAVSPEEWQAEAETVEIVEAAEEAVMVVSPAEEEQPVEEI
                     AQEQEKPTKEGFFARLKRSLLKTKENLGSGFISLFRGKKIDDDLFEELEEQLLIADVG
                     VETTRKIITNLTEGASRKQLRDAEALYGLLKEEMGEILAKVDEPLNVEGKTPFVILMV
                     GVNGVGKTTTIGKLARQFEQQGKSVMLAAGDTFRAAAVEQLQVWGQRNNIPVIAQHTG
                     ADSASVIFDAIQAAKARNIDVLIADTAGRLQNKSHLMEELKKIVRVMKKLDVEAPHEV
                     MLTIDASTGQNAVSQAKLFHEAVGLTGITLTKLDGTAKGGVIFSVADQFGIPIRYIGV
                     GERIEDLRPFKADDFIEALFARED"
     gene            21699..22367
                     /gene="ftsE"
                     /locus_tag="J1Y83_000891"
     CDS             21699..22367
                     /gene="ftsE"
                     /locus_tag="J1Y83_000891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HBA7629067.1"
                     /translation="MIRFEHVSKAYLGGRQALQGVTFHMQPGEMAFLTGHSGAGKSTL
                     LKLICGIERPSAGKIWFSGHDITRLKNREVPFLRRQIGMIFQDHHLLMDRTVYDNVAI
                     PLIIAGASGDDIRRRVSAALDKVGLLDKAKNFPIQLSGGEQQRVGIARAVVNKPAVLL
                     ADEPTGNLDDALSEGILRLFEEFNRVGVTVLMATHDINLISRRSYRMLTLSDGHLHGG
                     VGHE"
     gene            22360..23418
                     /gene="ftsX"
                     /locus_tag="J1Y83_000892"
     CDS             22360..23418
                     /gene="ftsX"
                     /locus_tag="J1Y83_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsX"
                     /protein_id="HBA7629068.1"
                     /translation="MNKRDAINHIRQFGGRLDRFRKSVGGSGDGGRNAPKRAKSSPKP
                     VNRKTNVFNEQVRYAFHGALQDLKSKPFATFLTVMVIAISLTLPSVCYMVYKNVNQAA
                     TQYYPSPQITVYLQKTLDDDAAAGVVAQLQAEQGVEKVNYLSREDALGEFRNWSGFGG
                     ALDMLEENPLPAVAVVIPKLDFQGTESLNTLRDRITQINGIDEVRMDDSWFARLAALT
                     GLVGRVSAMIGVLMVAAVFLVIGNSVRLSIFARRDSINVQKLIGATDGFILRPFLYGG
                     ALLGFSGALLSLILSEILVLRLSSAVAEVAQVFGTKFDINGLSFDECLLLLLVCSMIG
                     WVAAWLATVQHLRHFTPE"
     gene            23663..24517
                     /gene="rpoH"
                     /locus_tag="J1Y83_000893"
     CDS             23663..24517
                     /gene="rpoH"
                     /locus_tag="J1Y83_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007899894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoH"
                     /protein_id="HBA7629069.1"
                     /translation="MTDKMQSLALAPVGNLDSYIRAANAWPMLSADEERALAEKLHYH
                     GDLEAAKTLILSHLRFVVHIARNYAGYGLPQADLIQEGNIGLMKAVRRFNPEVGVRLV
                     SFAVHWIKAEIHEYVLRNWRIVKVATTKAQRKLFFNLRKTKQRLGWFNQDEVEMVARE
                     LGVTSKDVREMESRMAAQDMTFDLSSDDDSDSQPMAPVLYLQDKSSNFADGIEDDNWE
                     EQAANRLTDAMQGLDERSQDIIRARWLDEDNKSTLQELADRYGVSAERVRQLEKNAMK
                     KLRAAIEA"
     gene            24788..25891
                     /locus_tag="J1Y83_000894"
     CDS             24788..25891
                     /locus_tag="J1Y83_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     substrate-binding protein"
                     /protein_id="HBA7629070.1"
                     /translation="MNIKGKALLAGCIALAFSNMALAEDIKVAVVGAMSGPVAQYGDQ
                     EFTGAEQAVADINAKGGIKGNKLQIVKYDDACDPKQAVAVANKVVNDGIKYVIGHLCS
                     SSTQPASDIYEDEGILMITPAATAPELTARGYQLILRTTGLDSDQGPTAAKYILEKVK
                     PQRIAIVHDKQQYGEGLARAVQDGLKKGNANVVFFDGITAGEKDFSTLVARLKKENID
                     FVYYGGYHPEMGQILRQARAAGLKTQFMGPEGVANVSLSNIAGESAEGLLVTKPKNYD
                     QVPANKPIVDAIKAKKQDPSGAFVWTTYAALQSLQAGLNQSDDPAEIAKYLKANSVDT
                     VMGPLTWDEKGDLKGFEFGVFDWHANGTATDAK"
     gene            complement(26079..26462)
                     /gene="panM"
                     /locus_tag="J1Y83_000895"
     CDS             complement(26079..26462)
                     /gene="panM"
                     /locus_tag="J1Y83_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000778808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase autocleavage activator
                     PanM"
                     /protein_id="HBA7629071.1"
                     /translation="MKLTIIRLENFSDQDRIDLQKIWPEYSPSSLQVDDNHRIYAARF
                     NERLLAAVRVTLSGTEGALDSLRVREVTRRRGVGQYLLEEVLRNNPGVSCWWMADAGV
                     EDRGVMTAFMRALGFTAQQGGWEKH"
     gene            26885..27994
                     /gene="livk"
                     /locus_tag="J1Y83_000896"
     CDS             26885..27994
                     /gene="livk"
                     /locus_tag="J1Y83_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter substrate-binding protein LivK"
                     /protein_id="HBA7629072.1"
                     /translation="MKRNAKTIIAGMIALTISHTAMADDIKVAVVGAMSGPIAQWGDM
                     EFNGARQAIKDINAKGGIKGDKLVGVEYDDACDPKQAVAVANKIVNDGIKYVIGHLCS
                     SSTQPASDIYEDEGILMISPGATNPELTQRGYQYIMRTAGLDSSQGPTAAKYILETVK
                     PQRIAIIHDKQQYGEGLARSVQDGLKAANANVVFFDGITAGEKDFSALIARLKKENID
                     FVYYGGYYPEMGQMLRQARSVGLKTQFMGPEGVGNASLSNIAGDAAEGMLVTMPKRYD
                     QDPTNQGIVDALKADKKDPSGPYVWITYAAVQSLATALERTGSDEPLALVKDLKANGA
                     NTVIGPLNWDEKGDLKGFDFGVFQWHADGSSTAAK"
     gene            28042..28968
                     /gene="livH"
                     /locus_tag="J1Y83_000897"
     CDS             28042..28968
                     /gene="livH"
                     /locus_tag="J1Y83_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010436488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter permease LivH"
                     /protein_id="HBA7629073.1"
                     /translation="MSEQFLYFLQQMFNGVTLGSTYALIAIGYTMVYGIIGMINFAHG
                     EVYMIGSYVSFMIIAALMMLGIDTGWLLVAAGFVGAIVIASAYGWSIERVAYRPVRNS
                     KRLIALISAIGMSIFLQNYVSLTEGSRDVALPSLFNGQWVVGHSENFSASITTMQAVI
                     WIVTFLAMLALTIFIRYSRMGRACRACAEDLKMASLLGINTDRVIALTFVIGAAMAAV
                     AGVLLGQFYGVINPYIGFMAGMKAFTAAVLGGIGSIPGAMIGGLILGIAEALSSAYLS
                     TEYKDVVSFALLILVLLVMPTGILGRPEVEKV"
     gene            28965..30242
                     /locus_tag="J1Y83_000898"
     CDS             28965..30242
                     /locus_tag="J1Y83_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter permease LivM"
                     /protein_id="HBA7629074.1"
                     /translation="MKPMHIAMALLSAAMFFVLAGVFMGVQLELDGTKLVVDTASEIR
                     WQWVFIGTAVVFFFQLLRPAFQKGLKSVSGPKFILPAIDGSTVKQKLFLVALLVLAVA
                     WPFMVSRGTVDIATLTMIYIILGLGLNVVVGLSGLLVLGYGGFYAIGAYTFALLNHYY
                     GLGFWTCLPIAGLMAAAAGFLLGFPVLRLRGDYLAIVTLGFGEIVRILLLNNTEITGG
                     PNGISQIPKPTLFGLEFSRTAREGGWDTFSNFFGLKYDPSDRVIFLYLVALLLVVLSL
                     FVINRLLRMPLGRAWEALREDEIACRSLGLSPRRIKLTAFTISAAFAGFAGTLFAARQ
                     GFVSPESFTFAESAFVLAIVVLGGMGSQFAVILAAVLLVVSRELMRDFNEYSMLMLGG
                     LMVLMMIWRPQGLLPMTRPQLKLKNGAAKGEQS"
     gene            30239..31006
                     /gene="livG"
                     /locus_tag="J1Y83_000899"
     CDS             30239..31006
                     /gene="livG"
                     /locus_tag="J1Y83_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000084170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter ATP-binding protein LivG"
                     /protein_id="HBA7629075.1"
                     /translation="MSQPLLSVNGLMMRFGGLLAVNNVNLELYPQEIVSLIGPNGAGK
                     TTVFNCLTGFYKPTGGTILLRDQHLEGLPGQQIARMGVVRTFQHVRLFREMTVIENLL
                     VAQHQQLKTGLFSGLLKTPSFRRAQSEALDRAATWLERIGLLEHANRQASNLAYGDQR
                     RLEIARCMVTQPEILMLDEPAAGLNPKETKELDELIAELRNHHNTTILLIEHDMKLVM
                     GISDRIYVVNQGTPLANGTPEQIRNNPDVIRAYLGEA"
     gene            31008..31721
                     /gene="livF"
                     /locus_tag="J1Y83_000900"
     CDS             31008..31721
                     /gene="livF"
                     /locus_tag="J1Y83_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312328.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter ATP-binding protein LivF"
                     /protein_id="HBA7629076.1"
                     /translation="MEKVMLSFDKVSAHYGKIQALHEVSLHINQGEIVTLIGANGAGK
                     TTLLGTLCGDPRATSGRIVFDDKDITDWQTAKIMREAVAIVPEGRRVFSRMTVEENLA
                     MGGFFAERDQFQERIKWVYELFPRLHERRVQRAGTMSGGEQQMLAIGRALMSNPRLLL
                     LDEPSLGLAPIIIQQIFDTIEQLREQGMTIFLVEQNANQALKLADRGYVLENGHVVLS
                     DTGDALLANEAVRSAYLGG"
     gene            31809..32030
                     /locus_tag="J1Y83_000901"
     CDS             31809..32030
                     /locus_tag="J1Y83_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HBA7629077.1"
                     /translation="MHTMNYSEARQNLALVLETAATGTPVTITRRGHKAAVIISAEEF
                     ERYQKAKLDAEFDAIMAIHGSEIKELADK"
     gene            32027..32404
                     /locus_tag="J1Y83_000902"
     CDS             32027..32404
                     /locus_tag="J1Y83_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HBA7629078.1"
                     /translation="MTIQFISAEEIIYFHDKLLSVTPGVTGMSDLGRAEALLYRVLNK
                     YEYEGVSDLWLLAAMHLLAISRGHIFNDGNKRTALFITLLCLKRNGIILAANPEFVEL
                     TVDVAAGKVSLEDIVTRLRQRGT"
     gene            32613..33929
                     /gene="ugpB"
                     /locus_tag="J1Y83_000903"
     CDS             32613..33929
                     /gene="ugpB"
                     /locus_tag="J1Y83_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     substrate-binding protein UgpB"
                     /protein_id="HBA7629079.1"
                     /translation="MKPLHYTASALALGLALMGNAQAVTTIPFWHSMEGELGKEVDSL
                     AQRFNAENPDYKIVPTYKGNYEQNLSAGIAAFRTGNAPAILQVYEVGTATMMASKAIK
                     PVYDVFKEAGIQFDESQFVPTVSGYYSDSKTGHLLSQPFNSSTPVLYYNKDAFKKAGL
                     DPEQPPKTWQDLADYAAKLKASGMKCGYASGWQGWIQLENFSAWNGLPFASKNNGFDG
                     TDAVLEFNKPEQVKHIAMLEEMNKKGDFSYVGRKDESTEKFYNGDCAMTTASSGSLAN
                     IREYAKFNYGVGMMPYDADAKDAPQNAIIGGASLWVMQGKDKETYTGVAKFLDFLAKP
                     ENAAEWHQKTGYLPITKAAYDLTREQGFYEKNPGADIATRQMLNKPPLPFTKGLRLGN
                     MPQIRVIVDEELESVWTGKKTPQQALDTAVERGNQLLRRFEKSTKS"
     gene            34027..34914
                     /gene="ugpA"
                     /locus_tag="J1Y83_000904"
     CDS             34027..34914
                     /gene="ugpA"
                     /locus_tag="J1Y83_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000099274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter permease
                     UgpA"
                     /protein_id="HBA7629080.1"
                     /translation="MSSSRPVFRSRWLPYLLVAPQLIITVIFFIWPAGEALWYSLQSV
                     DPFGFSSQFVGLDNFVTLFHDSYYLDAFWTTIKFSTFVTVSGLLVSLFFAALVEYIVR
                     GSRFYQTLMLLPYAVAPAVAAVLWIFLFNPGRGLITHFLAEFGYDWNHAQNSGQAMFL
                     VVFASVWKQISYNFLFFYAALQSIPRSLIEAAAIDGAGPIRRFFKIALPLIAPVSFFL
                     LVVNLVYAFFDTFPVIDAATSGGPVQATTTLIYKIYREGFTGLDLASSAAQSVVLMFL
                     VIVLTVVQFRYVESKVRYQ"
     gene            34911..35756
                     /gene="ugpE"
                     /locus_tag="J1Y83_000905"
     CDS             34911..35756
                     /gene="ugpE"
                     /locus_tag="J1Y83_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000572177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter permease
                     UgpE"
                     /protein_id="HBA7629081.1"
                     /translation="MIENRPWLTIFSHTMLILGIAVILFPLYVAFVAATLDKQEVYAA
                     PMTLIPGTHLLENIHNIWVNGVGTNSAPFWRMLLNSFVMAFSITLGKITVSMLSAFAI
                     VWFRFPLRNLFFWMIFITLMLPVEVRIFPTVEVIANLKMLDSYAGLTLPLMASATATF
                     LFRQFFMTLPDELVEAARIDGASPMRFFCDIVFPLSKTNLAALFVITFIYGWNQYLWP
                     LLIITDVDLGTTVAGIKGMIATGEGTTEWNSVMAAMLLTLIPPVVIVLVMQRAFVRGL
                     VDSEK"
     gene            35758..36828
                     /locus_tag="J1Y83_000906"
     CDS             35758..36828
                     /locus_tag="J1Y83_000906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312323.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate import ATP-binding
                     protein UgpC"
                     /protein_id="HBA7629082.1"
                     /translation="MAGLKLQAVTKSWDGKTQVIKPLTLDVADGEFIVMVGPSGCGKS
                     TLLRMVAGLERVTTGDIWIDRKRVTEMEPKDRGIAMVFQNYALYPHMSVEENMAWGLK
                     IRGMGKQQIAERVKEAARILELDGLLKRRPRELSGGQRQRVAMGRAIVREPAVFLFDE
                     PLSNLDAKLRVQMRLELQQLHRRLKTTSLYVTHDQVEAMTLAQRVMVMNGGVAEQIGT
                     PVEVYEKPASLFVASFIGSPAMNLLAGRVNNEGTHFELDGGITLPLNGGYRQYAGRKM
                     TLGIRPEHIALSSQAEGGVPLVMDTLEILGADNLAHGRWGEQKLVVRLAHQERPTAGS
                     TLWLHLPENQLHLFDGETGQRV"
     gene            36825..37568
                     /gene="ugpQ"
                     /locus_tag="J1Y83_000907"
     CDS             36825..37568
                     /gene="ugpQ"
                     /locus_tag="J1Y83_000907"
                     /EC_number="3.1.4.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HBA7629083.1"
                     /translation="MSNWPYPRIVAHRGGGKLAPENTLAAIDVGAKYGHKMIEFDAKL
                     SKDGEIFLLHDDNLERTSNGWGVAGELNWQDLLRVDAGSWYSKAFKGEPLPLLSQVAE
                     RCREHGMMANIEIKPTTGTGPLTGKTVALAARELWTGMTPPLLSSFEIDALEAAQQAA
                     PELPRGLLLDEWRDDWRELTARLGCVSIHLNHKLLDKARVMQLKDAGLRILVYTVNKP
                     QRAAELLRWGVDCICTDAIDVIGPNFTAQ"
     gene            complement(37555..37995)
                     /locus_tag="J1Y83_000908"
     CDS             complement(37555..37995)
                     /locus_tag="J1Y83_000908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2756 family protein"
                     /protein_id="HBA7629084.1"
                     /translation="MKRLLLLTALLPFVGFAQPINTLNNPNQPGYQIPSQQRMQTQMQ
                     TQQIQQKGMLNQQLKTQTQLQQQHLENQINNNSQRVLQSQPGERNPARQQMLPNTNGG
                     MLNSNRNPDSSLNQQHMLPERRNGDMLNQPSTPQPDIPLKTIGP"
     gene            38115..39848
                     /gene="ggt"
                     /locus_tag="J1Y83_000909"
     CDS             38115..39848
                     /gene="ggt"
                     /locus_tag="J1Y83_000909"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HBA7629085.1"
                     /translation="MIKPTFLRRVAIAALLTGSCFSTVAAPPVSYGVEEDVFHPVRAK
                     QGMVASVDATATQVGVDILKEGGNAVDAAVAVGYALAVTHPQAGNLGGGGFMLIRSKN
                     GNTTAIDFREMAPAKATRDMFLDDQGNPDSKKSLTSHLASGTPGTVAGFSLALDKYGT
                     MPLNKVVQPAFKLARDGFIVNDALADDLKTYGSEVLPNHENSKAIFWKEGEPLKKGDK
                     LVQANLAKSLEMIAENGPDEFYKGTIAEQIAQEMQKNGGLISKEDLAAYKAVERTPIS
                     GDYRGYQVYSMPPPSSGGIHIVQILNILENFDMQKYGFGSADAMQIMAEAEKYAYADR
                     SEYLGDPDFVKVPWQALTNKAYAKSIADQIDINKAKPSSEIRPGKLAPYESNQTTHYS
                     VVDKDGNAVAVTYTLNTTFGTGIVAGESGILLNNQMDDFSAKPGVPNVYGLVGGDANA
                     VGPNKRPLSSMSPTIVVKDGKTWLVTGSPGGSRIITTVLQMVVNSIDYGMNVAEATNA
                     PRFHHQWLPDELRVEKGFSPDTIKLLEAKGQKVALKEAMGSTQSIMVGPDGELYGASD
                     PRSVDDLTAGY"
     gene            complement(39886..40170)
                     /locus_tag="J1Y83_000910"
     CDS             complement(39886..40170)
                     /locus_tag="J1Y83_000910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA7629086.1"
                     /translation="MITYHDAFAKANNYLDDANLPVVITLHGRFSQGWYFCFEAREFL
                     ETGDEAARLAGNAPFIVDKGSGEIHSLGTAKPLEEYLQEYEIKKATFGLP"
     gene            complement(40370..40525)
                     /locus_tag="J1Y83_000911"
                     /pseudo
     CDS             complement(40370..40525)
                     /locus_tag="J1Y83_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518820.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         9433 a        10457 c        11364 g         9386 t
ORIGIN      
        1 cggtatttat tcgcacatca ggcgataata acgctatgtg atagtcatca ggtttccctg
       61 ttacggaaac ctgtgcaaca gaatctgtga acctgtacag aatatttttg gaaaaaatac
      121 cagcgaacgg aagaacatcg cgatgtatca caagttgatg gcaatcaaat agacaacatg
      181 aatataactg ttagtattta attgatttca cttatgagta tgcgtgtttc atttacggcc
      241 aggaatgctg atgatgacaa cattactacc cgttttcact aagccttccc cattggcgct
      301 caatgctctg tgcgctggtc gtatttgcca tttccttctt atcccggatg gaagaatccg
      361 ctgaacatcg ttcggtggca gttgtaacac gcgtcgattg cttacaggga ttatggcgga
      421 tcgattatgg ataagagtaa gcgccatctg gcgtggtggg ttgtcggggc actggcggtg
      481 gctgccgtcg tggcgtggtg gctgttgcgc ccggcgggtg tgccggaagg ctttgctgtc
      541 agcaatgggc gcattgaagc gacggaagtg gatatcgcca gcaaaattgc cgggcgtatc
      601 gacaccattc tggtgaaaga aggccagttt gttcgagaag gtgaagtgct ggcgaagatg
      661 gatactcgcg tgttgcagga acagcgactg gaagccatcg cgcaaatcaa agaagcacaa
      721 agtgccgttg ccgccgcgca ggctttgctg gagcaacgcc aaagcgaaac tcgtgccgca
      781 cagtcgctgg ttaatcaacg ccaggccgaa ctggactccg ttgccaaacg tcatacgcgt
      841 tcccgctcgc tcgcccaacg aggggctatt tctgcgcaac agctggatga cgatcgcgcc
      901 gccgctgaga gcgcccgagc tgcgctggaa tcagcaaaag cccaggtttc ggcttctaaa
      961 gccgctatag aagcggcacg caccaatatc attcaggcgc aaacacgcgt cgaagccgca
     1021 caagccactg aacggcgcat tgccgcagat atcgatgaca gcgaactgaa agccccgcgt
     1081 gacggacgcg tgcagtatcg ggttgccgag ccaggggaag tgctggcggc aggcggtcgg
     1141 gtgctgaata tggtcgatct cagcgacgtc tatatgacct tctttctgcc aaccgaacag
     1201 gcgggcacgc tgaaactggg cggtgaagca cgtttgattc ttgatgctgc accagatctg
     1261 cgtattcccg cgaccatcag ttttgtcgcc agcgtcgccc agttcacgcc aaaaaccgtc
     1321 gaaaccagcg atgagcggct gaaattgatg ttccgtgtca aagcgcgtat cccaccggaa
     1381 ttactccagc agcatctgga atatgtcaaa accggattgc cgggcgtagc gtgggtgcgg
     1441 gtgaatgaag aacttccgtg gcctgacgac ctggtggtga ggttgccgca atgacgcatc
     1501 tggaactggt tcccgtcccg cctgtcgcgc aactggcggg cgtgagccag cattatggaa
     1561 aaaccgttgc gctgaacaat atcacactcg atattccggc ccgttgcatg gtcgggctga
     1621 ttggaccgga tggtgtcggt aaatcgagct tgttgtcgtt gatttccggt gcccgcgtca
     1681 ttgagcaggg cagcgtgatg gtgctgggcg gcgatatgcg cgacccgaag catcgccgcg
     1741 acgtctgccc gcgcatcgcc tggatgccgc aggggctggg caaaaatctc taccacacct
     1801 tgtcggtgta tgaaaacgtc gattttttcg cccgcctgtt cggtcacgac aaagcggagc
     1861 gggaagtgcg tattaatgag ctgctgacca gcaccggatt agcaccgttt cgcgatcgtc
     1921 cggcagggaa actctccggt gggatgaagc aaaaacttgg gctgtgctgt gcgttaatcc
     1981 acgacccgga attgttgatt cttgatgagc caacaacggg ggttgacccg ctctcccgcg
     2041 cccagttctg ggatctgatc gacagcattc gccagcggca gagcaatatg agcgtgctgg
     2101 ttgccaccgc ctatatggaa gaggcggaac gcttcgactg gctggtagcg atgaatgccg
     2161 gagaagtgct ggcaactggc agcgccgaag agttgcggca gcaaacgcaa agcgccacgc
     2221 tggaagaagc atttataaat ctgttaccgc aagcacaacg gcaggcgcat caggcggtag
     2281 tgatcccacc gtatcaacct gaaaacgcag agattgccat cgaagcgcgc gatctgacca
     2341 tgcgttttgg ttcctttgtc gccgttgatc acgtcaattt tcgcattcca cgcggggaga
     2401 tttttggttt tcttggttcg aacggctgcg gtaaatccac caccatgaaa atgctcaccg
     2461 gactgctgcc cgccagcgaa ggtgaggcgt ggctgttcgg gcaaccggtt gatccaaaag
     2521 atatcgatac tcgtcgccgg gtgggctata tgtcgcaggc gttttcgctc tataacgaac
     2581 tcaccgtgcg gcaaaacctg gagttacatg ctcgtctgtt tcatatcccg gaagcggaaa
     2641 ttcccgcgcg agtggctgaa atgagcgagc gttttaagct caacgacgtc gaagatgtat
     2701 tgccggagtc attgccgctc ggcattcgcc agcggctatc gctggcggtg gcggtgattc
     2761 atcgcccgga gatgttaatc cttgatgaac cgacttctgg cgtcgatccg gtggcgaggg
     2821 atatgttctg gcagttgatg gtggatctct cgcgccagga caaagtgact atcttcatct
     2881 ccacccactt tatgaacgaa gcggaacgtt gtgaccgtat ctcactgatg cacgccggaa
     2941 aagtgctcgc cagcggtaca ccgccggaac tggttgagaa acgcggagcc gccagtctgg
     3001 aagaggcatt tatcgcttat ttgcaggaag cggcagggca gagcaacgaa gctgaagcgc
     3061 cgccggtggt acacgacacc acccacgcgc cacgtcaggg attcagcctg cgccgtctgt
     3121 ttagctacag ccgccgcgaa gcgctggaac tgcgacgcga tccggtacgt tcgacgctgg
     3181 cgctgatggg aacggtgatc ctgatgctga taatgggtta cggcatcagt atggatgtgg
     3241 aaaacctgcg ctttgcggtg cttgaccgcg atcaaaccgt cagtagccag gcgtggacgc
     3301 tcaatctctc cggttcccgt tactttatcg aacagccacc gctcaccagt tatgacgagc
     3361 ttgatcgtcg gatgcgtgcg ggcgatatca cggtggcaat tgagatccct cccaatttcg
     3421 ggcgcgatat cgcgcgtggt acgcctgtgg aactcggtgt ctggattgac ggtgcgatgc
     3481 caagccgtgc cgaaacggta aaaggttacg tgcaggccat gcaccagagc tggctacagg
     3541 atgtggcgag ccgacaatcc acaccctcca gccaaagcgg gctgatgaat attgagacgc
     3601 gctatcgcta taacccggac gtaaaaagcc tgccagcgat tgttccggcg gtgatcccgc
     3661 ttctgctgat gatgatcccg tcaatgctaa gcgcccttag cgtggtgcgg gaaaaagagt
     3721 tggggtcgat tatcaacctt tacgtgactc ccaccacccg tagcgaattt ttgcttggca
     3781 aacagctgcc atacattgcg ctggggatgc tgaacttttt cctgctctgc gccctgtcgg
     3841 tgtttgtgtt tggcgtaccg cataaaggca gtttcctgac tctcaccctg gcggcgctgc
     3901 tgtatatcat catcgccacc ggaatggggc tgctgatctc cacctttatg aaaagccaga
     3961 ttgccgccat tttcggtacg gcgattatca cgttgatccc ggcgacacag ttttccggga
     4021 tgatcgatcc ggtagcttcg ctggaaggtc caggacgttg gatcggcgag gtttacccga
     4081 ccagtcattt tctgactatc gcccgcggaa cgttctcgaa agcgctggat ctgactgatt
     4141 tgtggcaact ttttatcccg ttgctgatag ccataccgct ggtgatgggc ttaagcatcc
     4201 tgctgctgaa aaaacaggag ggatgatgcg ccatttacgt aatattttta atctgggtat
     4261 caaagagttg cgcagtctgc tcggtgataa agcgatgctg acgctgattg tcttctcgtt
     4321 tacggtatcg gtgtattcgt cagcgaccgt tacgccagga tcgttgaacc tcgcgccgat
     4381 cgccattgcc gatatggatc aatcgcagtt atcgaaccgg atcgttaaca gcttctatcg
     4441 cccgtggttt ttgccaccgg agatgatcac cgccgatgag atggatgccg ggctggacgc
     4501 cggacgttat accttcgcga taaatattcc gcctaatttt cagcgtgatg tcctcgccgg
     4561 acgccagccg gatattcagg tgaacgtcga tgccacgcgc atgagccagg catttaccgg
     4621 caatgggtat atccagaata ttatcaacgg tgaagtgaac agctttgtcg cgcgctaccg
     4681 tgataacagc gaaccgttgg tatcgctgga aacacggatg cgctttaacc cgaaccttga
     4741 tcccgcgtgg ttcggtgggg tgatggcgat catcaacaac attaccatgc tggcgattgt
     4801 attgaccgga tcggcgctga tccgcgagcg tgaacacggc acggtggaac acttactggt
     4861 gatgccgata acgccgtttg agatcatgat ggcaaaggtc tggtcgatgg ggctggtggt
     4921 gctggtggta tcgggattat cgctggtgct gatggtgaaa ggcgtgctgg gcgtaccgat
     4981 agaaggctcg atcccgctgt ttatgctggg cgtggcgctc agtctgtttg ccaccacgtc
     5041 aatcggcatt tttatgggga cgatagcgcg ttcgatgccg caactggggc tgctggtgat
     5101 tctggtgctg ctgccgctgc aaatgctttc cggtggatcc acgccacgcg aaagtatgcc
     5161 gcagatggtg caggacatta tgctgaccat gccgacgaca cactttgtta gcctcgcgca
     5221 ggccatcctc taccgtggtg ccggattcga aatcgtctgg ccgcagttcc tgacgctgat
     5281 agcgattggc ggcgcgtttt tcaccatcgc actgctgcga ttcaggaaga cgattgggac
     5341 aatggcgtga ttacggggtt aatcttcctt cggcaggcac tgcaaatgcc cgtgccgcac
     5401 gccgcgctgg gcgatgacgt catcggcaaa atgctgcacg tcgcccatgt cacctttcaa
     5461 cacggcgatt tccagacagt cgtcgtgatt aatgtgcaca tgcagagtag caactgagag
     5521 atcgtggtga tgatgctggg tggagacaat acggctggct aagtcgcgtt tttcgtgttc
     5581 atacacatac gacagcaccg cgaaaccttg tgtgccgtgt tgttgagtgg cttcctgcgc
     5641 cagagcgcta cgcagaatat cgcggatcgc ctcggaacgg ttgttataac cacgacgctg
     5701 gctcagactg tccagcgtct ccagtaaatc gtcatcaagc gtgatggtga ctcgttgcat
     5761 ttgcgttaaa ccttttctgt ggtgcgacgg cgcacgggga atgcgggtaa taccgcgttt
     5821 tgtagcacac gtccggcgtc agaggaaaag gttaatttat ctcccaccac ctgggtttcg
     5881 acgatttgtc cgttgtccat caccatcacc cgctgacaga agcgttccac caggcgtaag
     5941 tcgtgggtga tgaacaggca ggcggtgcca aactgttgtt gcagcttttt cagcaggcga
     6001 atgacgcccg cctgtaacac gagatcaagg ttcgaaaccg cttcatccag aatcagcagt
     6061 ttcggttcga ccgccagcgc gcgtgccagg cagacacgct ggagctggcc gccgcttaac
     6121 tgcggtgggc gtttgtcgag cacgctgtcg tcgagatcga ccgccttcag catttcactg
     6181 gcgcgcgcca gttgttcggc ttttttcagc gatagcaggt ggcgcatcgg ttcacgcagg
     6241 atctcgcgca cggttttgcg cggattcacg gcgctgatgg agtcctgaaa caccatttga
     6301 atatcacggc ggaacgcttt acgttgcgcg cgattgagct tcgccaacgg ttcgccgcgc
     6361 cagctaatat ttccctgtga gggcgactct aaccctacca gcagccgcgc gagggtactt
     6421 tttccgcagc cgctgcgccc cagcagggcg acggtttcac cgcttttcag ggccagggaa
     6481 acgttattca gcaccgcctg gtgttgatgt ttgccgctaa atccaccgtg cgcgtagtga
     6541 tgggaaaggt cggagacgtt aagtaaagtc atgatgccag ctccataccg tagagggcga
     6601 gatgcgcgga aaccaggctg cgcgttaccg catgtttggg ggcgttaaac agcgtttcta
     6661 catcgccctg ttcaacaatc ttaccgtgag acattaccgc cacatcgtct gccagacgcg
     6721 ctaccacgcc catatcatgg gtaaccagca gcattcccgg tgcttgtttt tgcataatgc
     6781 tttccagcag atcgaggatg cgcgcctgtg ctaccacgtc gagatcggtg gtcggttcat
     6841 cggcgatgat aaacggtgat tcgcacagca ccgccatcgc aatcatcatg cgctgcaaca
     6901 tgccgccgct catctcgaac gggtagagct tcagcacgcg cgcggcgttt tccagcccca
     6961 ccgcttctat ggcagcggta agcgtagcgc catcggcggg tttccccagc gccaggcagg
     7021 tttcacgcgc gtgggtgtgc atggtgtgca gcggattaaa ggcactgcgc gggttctgca
     7081 tgatggtggc aattttgatg ccgcgcaggg cgcagggaga aaccggtttt ccatcggcca
     7141 aaatttcccc cgccgtctgg cgaacgccag caggcagaat gcccagcgtc gcggcgcagg
     7201 tcagcgactt cccgctgcca ctaccgccga ctaacgccag cacacgccca cgttgcaggg
     7261 ttaacgatac gccgtgcacc agcggctgcg cggcctgtag cgcgatatta cggagttcaa
     7321 tctgttgtgg cattagtgtg cgtgctccgt caccagatga gggtccagat gatcgcgcag
     7381 tgcgtcaccc accaggttaa aggccatcac gctgataaac agcgccagcc ccggccagaa
     7441 catttgcagc ggctgggtcc agatatactg acgcgcgtcg ttaatcatca cgccccattc
     7501 ggcggtcggc gcggtcacgc cgagaccgag gaaggacatc cccgcgacgt gtagcatcat
     7561 atgaccgata tccagcgtcg ccagcaccag cagcgagggg atcaccgcac ctgccagatg
     7621 atcaataaac acccgcacat ggcccgcgcc agaaagccgt gatgccagca caaactcgcg
     7681 ctggcgcagt gaaatcacca gactgcgcac catgcgcgcg taccatgccc agtgcgaaag
     7741 ggcgatagcg ataattacgt tggtcaaccc ggtgccaagc acgccgacca taaagaacga
     7801 cagaatcgag gttgggaagg tcataaacat atccgcgacg cgcatggtgg cctgatcaac
     7861 gcgcccgcca atcagcccgg cgctgccgcc aataatcagc cctaatgtca gcaccaacag
     7921 caggcaggcc attaccgagc cgagcgacac gcgggtcgct gccatcagcc gcgagaaaat
     7981 atcgcgccct aaatgatcgg tgcccagcca gtgctgcgca tccggcgaga gcaggcgcga
     8041 cggcaaatca atagcctgag ggtcatacgg cagccaccac tggctggtga gcgcaatcag
     8101 cgccagcagg gcgataatga tcagcgccag gcgaaccgac cagcgggagg agaggaaaaa
     8161 gttcacgcgt gcgctccttc atgacgacga atacgcgggt ccagcgcggc gttgagcaaa
     8221 tcgacaatca aattacagac cacaaaaacc accaccatca tcagcgtaaa gcattggatc
     8281 accggatagt cacggttaaa aatcgccgac accgcatagc gcccgacgcc cggccaggca
     8341 aagatgtttt cgataatcat cgtcccgcca atcagttcgc caatgtgcat ccccacggca
     8401 gtgatcatcg gcagcgaggc attgcgcaga atatggcggc gttcggtctg tttgtcgctc
     8461 agaccacgca gtcgtgccca ggtgacgtga cgctgaccgg cgacgtccag catactggcg
     8521 cgcagcaaac gcgcgttaat cgccagcgac ataaaggcga tggataccgc aggcaaaatg
     8581 atgtgctgcc agccgccgta acccatcgcg ggtagccatt gcagatacac cgaaaacgcc
     8641 atcaccagta aaaacgccag ccagaagtta ggcatcgaca cgccgagaaa cgcgatgaaa
     8701 cggacggcga aatccggcag acgatcgcga tggcgcgccg cccagatacc gagcggcacg
     8761 gaggtgagca gaattaatac cagcgccgca cctgcaagtt ccagcgtggc gggcaggaaa
     8821 ttcagcatat cgtccagcac cgggcgttgg ctggcgaatg agataccaaa gtcaagatgc
     8881 agcgccttcc acaaccaggt gccgtactgg acatacagcg gctgatccag ccccagcatg
     8941 gtgcgggtag aggccagcat ctccggcgtc ggcggcaggt tagataaacg caaataatca
     9001 agcgccgggt cgccggtgcc gaggcgcagc atcaaaaaaa tgatcaccga ggcggcgagc
     9061 accatcggga tcagcagcag aaagcggcgt aatacataac gcaacattaa ggtttcaccg
     9121 gtttaatctg ttcgaacggg atttcggtgg cgatcggcgc gtaggggatg ttacccagtt
     9181 ccggttttga aaccaccatc attgagatgt aactgatagg cagataaacc gcctcgtcat
     9241 gcagacgggt cagaatgtcg cgatacagcg cctgacgttg cgtttcgtca tgggtcgcca
     9301 gcacttcgcc gatctcttta tcaatcagcg gcttgtcggc taaaccttgc tgtgcctgga
     9361 agtcagcgtg cgacggtacg cgcattgaac tcaggaaggc gtgtggatcg tatggcgcgc
     9421 cccaggtgcg gtggaaaatc atgccaaaac gaccgtcgcg ctgacgagca tagatactgc
     9481 tctcttcttc gccaatcagc gagacatctg cgccaatctg gcgcatatca gcctgaatga
     9541 tttccgccat cgatttgctt aacgcatcgg tgccgatgaa cgacagttca atgcgcagcg
     9601 gctgaccatt tttctcgcgg atgtctttgc ctgcaggcag cgtccaaccg gctttttcca
     9661 gcaacgcttt cgctttctgc ggatcgtact ggcgcggttt caggccgagg ttggcgtagg
     9721 gcacagaagg ggcaaacagg gtgtcggcga cctgctgggt accatacaac gcgttatcaa
     9781 tcagcgattt tttgttcacc gcgtaattaa gagcttcacg tactgccagc tcgttggtgg
     9841 gggctttggc ggtattgagc gccagcatca cggtttcgat cggctgtgac agttgagtgt
     9901 ggtaagccgg gttctggcta aagcgggcga aggtatcgag cggtaataac ccttcgtttc
     9961 cgtacagcag gtcgatatcg ccagtttcaa acgccaccgc gcgggtagtc gggtccggga
    10021 tcacgttaaa ggtgatcttt ttaatcgccg gcttttcgcc ccagtagttt tcgttacgga
    10081 cgaagacatc gtactgattc agtttcgatt cctgtaaaac ccacggcccg gtgccaatcg
    10141 gcgctttaat tccgttcatg gtttcgtggt ttttaaactg cgagggtgcg ataaaacgga
    10201 aaggacgggg cagggccagt tcttgcagga aaggatagta ggcgcttttc agggtgattt
    10261 gcagctcgtt tttgctgagt gctttgacat caacaatctg gtttgccagc tccagccagg
    10321 cgtgacgttg acggttatcg agcactgcac ggaagttttc tgccgccgcc tctgcatcaa
    10381 acggttcgcc gttggagaac ttcacgtcat cacgcagggt gaagatccag gttttaccat
    10441 cttctgaatg agtccagctt tttgccagcc acgggatcac cgaaccgtct gcctgatatt
    10501 tcaccagcgg ttcataaacc atgctttggg cgaacatttg gttaggcgtg taaaggtgcg
    10561 ggtttagtgg cccgacattc accggccagg cggtggtgat ttcatctggt gcagcggcat
    10621 ggacgataaa agacgcacaa gccagcagcg caaatagagt gcggcggagt gtagagaaca
    10681 tggtaacccc aatggattaa aatagatggc ggaaataagt atgacgattt taagtattcg
    10741 tcatactgat tacctgttct ggatcaatag atgggcagtc gggtgggcgg atcagttaac
    10801 tgaatcgatc cattgcacac tgtcggcggt gagcgtaaag ggggtagggg tgcagatcgc
    10861 caggcttaaa ttttctagct gacaatggct gactgacagc gaggagttat aggtgctgtc
    10921 tacgctgacg atttgccagg cgctgccgcc acgctgttta acgatggctt ctttgcgcgt
    10981 ccagatgcgc cagaacattt ccagttgctg atcaggatgc acggcgtcca tctcggcatg
    11041 ttccccgagg ctgaatacgg cgttcgccag ccagcgccag ttggcgcgcg ggcgaatcac
    11101 ttcgatatcg cagccgactt cgccttcatc gctcaacagc agggcgatat cgtcaccgct
    11161 atggcttaag ttgaaccata gcggcgtttc cggcgcgaat gcaggtttgc cttgttcgcc
    11221 atagatgatc tccggtagcg gggaaagcgt gtgcgaaagc aatgcacgtc ccgccagcca
    11281 gcgttcgcgt cgtggacctt gcggtgcttg ctcgcgtaaa cccggtggca gaggagctgc
    11341 gcttaaggtc gaaactttcc ccagaactat ccgatacata tcagggccaa cgtttaatgg
    11401 aaaatgaaag tgcgtatcgt aacacttgtc gcctcatccc ggtaaccgac ttttcggtct
    11461 gcctggcccc agtaaaatcg ccagtttgct gccgcctttg gtggtttcca tccagatttt
    11521 acacacgctg gttaatggca ccgaaagtag catccctacc gggccgagca gccatcccca
    11581 aattaacaat gaaagaaata ccaccatggt ggacatcccc aggcgatggc ccatcatccg
    11641 tggttctaaa atattgccga tgaccatatg gaccactaaa aacaatgcgc cgaccagaat
    11701 acattcgtaa ataccattaa acagcagcac ctgaatcatt ggcggtacgg cggaaattac
    11761 cgcaccgata ttgggcacgt agttgagcaa aaacgccagt actgcccaca tcagcgcaaa
    11821 ctgcaccccc atcagctcca gccccagcca gacgattatg cctgtccata aactgagtag
    11881 cgtctttaac gccagataat gcgaaacgcc tttaagtgcg cggtgtagtc cggcgatgtg
    11941 aatctgtgga ttattcaacg cgaaacgcat tttgtaaggg acgtggcgca cttcaaacag
    12001 cataaaaact acggtcatca ccagcaaaag cacgctcgcc attgccccgg aaagcccggt
    12061 cattagcgcc gtggtgaagg taaccacttt ttccgagtcc atccgctgta gtattcgctc
    12121 cggcgacata tgcaaattaa gaaaaggcaa catctcctgc aatttaaaaa gtttgcgcgt
    12181 cagctcctta ttaaacttcg gcagcataga gataaattcg ttaaacgatg ccgccagtac
    12241 gccgaccagc gcggtgagtg cgatcagcat caccacaacc acaatcgtaa tggcaacggg
    12301 gcgttgtact ccccgacgaa taaaccaggt gacgagcggg ttgagaacga tggcaaaaaa
    12361 cagcgccagt aatagctgca caatgatatc tgccgctgcg tgaatgcccg cgaggatcac
    12421 taccagcgag gccaacttga gcagaatgtg catgcccgtt ttatcgggtt gaggggtttc
    12481 cattggggct tccttgtgac tttttgtatt aagtgtagcg ggagtcacgc cagcaatatt
    12541 ctgattctta ctgctgattt tcacgtcagc acatggtaaa aatgaaacac tgttgtaaaa
    12601 atgtggtgat cctcatgccc gaacccgtag ccgaacccgc gctaaacgga ttgcgcctga
    12661 atttgcgcat tgtctccatt gtcatgttta acttcgccag ctacctcacc atcgggttgc
    12721 cgctcgctgt attaccgggc tatgtccatg atgtgatggg ctttagcgct ttctgggcag
    12781 gattggttat cagcctgcaa tatttcgcca ccttgctgag ccgtcctcat gccggacgtt
    12841 acgccgattt actgggaccc aaaaagattg tcgtcttcgg tttatgcggc tgctttttga
    12901 gcggtctggg atatctgacg gcaggattaa ccgccagtct gcccgtcatc agcctgttat
    12961 tactgtgcct ggggcgcgta atccttggga ttggacaaag ttttgccgga acgggatcga
    13021 ccctgtgggg tgttggcgtg gttggttcgc tacatatcgg gcgggtgatt tcgtggaacg
    13081 gtattgtcac ttacggggcg atggcgatgg gtgcgccgtt aggtgtcgtg ttttatcact
    13141 ggggcggatt gcaggcgtta gcgttaatca ttatgggcgt ggcgctggtg gccattttgt
    13201 tggcgatccc gcgtccgacg gtaaaagcca gtaaaggcaa accgctgccg tttcgtgcgg
    13261 tgcttgggcg cgtctggctg tacggtatgg cgctggcact ggcttccgcc ggatttggcg
    13321 ttatcgccac ctttatcacg ctgttttatg acgctaaagg ttgggacggt gcggctttcg
    13381 cgctgacgct gtttagctgt gcgtttgtcg gtacgcgttt gttattccct aacggcatta
    13441 accgtatcgg cggcttaaac gtggcgatga tttgctttag cgttgagata atcggcctgc
    13501 tactggttgg cgtggcgact atgccgtgga tggcgaaaat cggcgtctta ctggcggggg
    13561 cggggttttc gctggtgttc cctgcattgg gcgtagtggc ggtaaaagcg gttccgcagc
    13621 aaaatcaggg ggcggcgctg gcaacttaca ccgtatttat ggatttatcg cttggcgtga
    13681 ccggaccact ggctgggctg gtgatgagtt gggcgggcgt tccggtgatt tatctggcgg
    13741 cggcgggact ggttgcaatc gcgttattac tgacgtggcg attaaaaaaa cggcccccgg
    13801 tggaaatacc tgaggccgcc tcatcatctt aaaacttact gaataaccag cgtattaatg
    13861 atgttttctg cggtggtctg tgctttttgc tgattgtcag cgggcagcgt aatttgcatg
    13921 gttagcagtt gattacccac gttacccaga ataacggaag agtacgccgt ctggcctttc
    13981 gcggagataa tgctgtctaa ctgctgcatt ttgtgacctt tcagctcaat ggctttattg
    14041 gttaccactt gcagctgcgg atcgcggcta cgttgctgat cttccagacg cttcgccagc
    14101 accgccagat cttctttcgg atcatcgccc atgatgacga tgactgcttt ctgcccggtg
    14161 gcgtcggacc agacatgcat gttattggcc tgcgttccca gcttaccgct ctggtcggtc
    14221 atatccgctg gcagcgagaa acttaacttg ccatcaagca ggttgacggg attcccggtc
    14281 gcgttacttt ctgcgaccga accctgcgcc gtagcgttag tgtctttatc atcacaggcc
    14341 gcaagcccca taaccagcag gccaattccg acatatttaa ccagattgcg cattgacttc
    14401 ttcctttcga taaacggcca taacggctca ttcatccatc ttatcacaac tctgataacg
    14461 aacctttaac tcgcctgcaa agcgttgatt tcggatttat ctgccagtct tttcaacagc
    14521 atattgagta atacgccata cattggcagg aagaaaacga tactgattaa cactttgaaa
    14581 cagtaatcga ccagcgcgat ttccatccag tgttcagcca taaaggcatc cgggctacgc
    14641 cagaaggcaa tgaagaagaa ggccagcgtg tcgctgacgt taccgaacag tgtggacgct
    14701 gtcggtgcca gccaccagtg acgactctga cgcaggcggt taaaaacgtg cacgtcgagg
    14761 atctgcccca gcgcgtaggc catgaaactg gcggtggcga tacgggcgac aaacaggttg
    14821 aagtgggcga gcgcgccgaa tccttgccat gaccccatat aaaacagcga tgagatgacg
    14881 taggagatca gcaatgcggg gatcattacc gcaaagatga tgcgacgagc cagcggcgcg
    14941 ccaaaaatac gcacggtcag gtcagtagcg agaaagataa acggaaagct aaacgcgccc
    15001 caggtggtat ggaaaccaaa aatggagacg ggaagctgaa ccagatagtt actggaggtg
    15061 atcaccagca gatgaaataa cgataaccag aacaacgcct tatagcgttg cgtttgcgag
    15121 aaaacgttca tattgtacct ttttgattaa ccattggggt gagggaaccc aatacgtacg
    15181 acacgtctgt taccttaaca taataacgtg ctgacccttt tttcgagccg ccgcatgata
    15241 ctgctttacg ttgacaatgc aatggttaat tttcacgcaa tcgttaacct ggtttgctta
    15301 cggactcaca gggcgtaaac tagcgccgtt tttttatgtg atgagaagaa aatgaccgat
    15361 ctcttttcca gtcctgacca tacactcgac gcgcttggct tgcgctgccc ggaaccggtg
    15421 atgatggtgc gtaaaacggt gcgcaatatg caaccaggcg aaacgttgtt gattattgcc
    15481 gacgatccgg cgacgacccg cgatattcct gggttttgta cctttatgga acacgaactg
    15541 gttgcgaaag agacggatgg actgccttat cgttatttga ttcgtaaagg cggttaatgg
    15601 gggaagatgg gcttcgatgc cgccttttcc cctcacccta accctctccc cagaggagcg
    15661 aggggaccga tcgcgctcaa tgttgctgct tgaaaccggt ttgtaggccg gataaggcgt
    15721 tcaagctgca tctggcgata cggtgcacaa tgcctgatgc gacgctgtcg cgtcttatca
    15781 tgcctatgag ttcgatgctc tgtgtaggcc ggataaggtg ttcacgccgc atccggcatt
    15841 gttggaccgt agactgatag cctctttatt tcctgcgcaa taaccgtaac gcattcgccg
    15901 ttaccagcac cgtcgcaccg gtatctgcca gcactgccag ccacaggccg gtcatcccta
    15961 acagcgtggt gacgaggaaa acgcctttca gccccagcgc aatagtgatg ttctggcgga
    16021 tattggcgtg agtggcgcgt gccagttcaa tcatttgcac cagaccgcgc aggtggttat
    16081 gggttaatgc tgcatctgcg gtttccaggg ccacgtccgt gccactgccc attgcaatcc
    16141 cgatggcggc agctttcatc gctggcgcgt cgttaatacc gtcgccgacc atcgccagcg
    16201 gcgcatgttg attcagctcg gtcaccgctt taactttatc ttccggcaag aggcccgctt
    16261 taaattccag ccccagttcc ccggcgattg ccgctgctgc gcgtggatta tcgccggtga
    16321 ggatcacccc tttgacaccc agcgcgttca gttcactgat agcagttgca gcatcggcgc
    16381 gcagggtatc ctgcaacgca atgacaccca gcacgtcatc attacgtact accagcacta
    16441 ccgtttgccc ggcgctttcc agttcattaa tcaaaccagc aaatgcatca gcaggatgtt
    16501 ttccggcagc gcatatcaac acgcgctcac cgttaacctg cgcttcaatg ccagacccga
    16561 ccagcgcccg ctgtgattgg gcggtgggaa tggcgagttc agcaacctgt gcttcgcgca
    16621 caatggcctg tgccagtgga tgcgtcgcgc cttgctcgac cgccgccgcc agtgtcagca
    16681 gttcagattc actaataccc gttgccggat gaatcgcggt aacgcgcggt ttaccgacgg
    16741 tcagcgtacc ggttttatca aacgccacca gggtaacacg acccagctgc tccagcgccg
    16801 cgccgccttt aatcaacgcc ccacgacgcg ctgctgccgc cagcccggag gtaatcgccg
    16861 cgggcgtgga gataactaaa gcacacgggc aaccaatcag cagcagcgtc agccctttat
    16921 aaatccactc ctgccagctg gcggcaaaca gcagcggcgg caccagcgtt accagcaggg
    16981 cgacggccat aatcgctggc gtgtaaatac ggctgaaacg gtcgataaac cgctcaatag
    17041 gtgcgcggcg ctcttcggct tcttcaatca gtttcagaat ccggtcaatg gcgctggcac
    17101 ccggttctga cagcacttcc agcgttacca gacggtctac gctggtggtg cctgcaggaa
    17161 ctttatcgcc cgtcgcgcgc tccacaggaa tggattctcc ggtcagggca ctttcatcaa
    17221 aactggcaaa cggtgagagc agtttaccgt cggcaggcaa acgcccacct gccgcaactt
    17281 caatcacatc gccaggccgc aggctgttta ttgccacctc ttcacgctca ccgttacgca
    17341 gacgcgtggc ggtttctggc ttcagcgcca ttaacgcgct aacgccctga cgcgcgcggc
    17401 tggcggccca gccttccagt cgttcaccaa tcaaaaacag cagcaacacc atcgcagctt
    17461 cagccgttgc gccaataaac agcgcaccaa tagcggctac gctcattaag gtttcaatgg
    17521 cgaagtagct gccagatttg atcagccgta atgcctgacg agcaattggg tacagcccaa
    17581 ccagcgtggt cgcgataaac gccagttgac caaacggatg attgaactgc tccagccccc
    17641 aactgattgc catcatcacg ataagcgtaa tcagcggcag attctctttc aggcgtgatt
    17701 cttgtggttc gtcggaggcc tgttcatcgc gcagggaata gcccgctttt tgcaccgcag
    17761 attcaacttg tgcccggatg tcattgtcgg catccaccac cagtttttcg gtggcgaaca
    17821 acacctgtac ctgattcacg cctgcaagct ggcgcacggc attttccact ttgcgcgcac
    17881 aggcggcgca gtccataccg ctgactttcc agctatagcg ggtgccggag acgttttcag
    17941 agagagttgg cgtgctggaa catgcgccgt cgcagcaaca gtcgttggtg ttctgtaccg
    18001 tagttagcgg tttgaacgca gcaaattgag gggctttctt gccgtgattg tcaggagtcg
    18061 acatggcatc ctccggttaa gttttttctc attaaccgaa ggatacactc tggagtcgac
    18121 tccagagtca agttttatca gagatacagc gagcggacga tgaggaagtg accagcaaag
    18181 tagcaggccg cggcaatggc gttatccgca cggaagcgtc ggcgatagtg actacccagc
    18241 cagacaaagt tactgataaa cagcaacgaa gcgccgacaa acgcagagag cgccggagcg
    18301 gtcggacgga agaaccacag ttcacctgcc agccacacca tcaccagcgt catgccgata
    18361 aacgtgcaga taggccaacg atactcttcc agacgcgtcc agataatcgc cagtaacagc
    18421 gcgcccagaa ccagcagcac caacggcaga ggccagaaga aagagagcgt catctgactg
    18481 gcgaaataga tggtgtacag caggtgcgag aggaaaaacg cgccgatggc gtacatcaga
    18541 cgttgacgtg gcaacagggt tagcgcatcg cccagcagtg aggcacacag ccccgccagc
    18601 accagatagc taatggcgtc gaacatcggc gcttgccagg ccagcaacag caggagaaga
    18661 agggttaacg gtttaaacac ccagcgttgc caggttggcc cacgatacga tgcatccaca
    18721 gatagccatg cggaaagaca gacagcgata aacgaccaaa gcatcttagt tccttgaatt
    18781 cgttattttt tcgtaagcgg aatgcctacg gtcttgtttc cagtttagtg gcgagggtgg
    18841 acggatgaca acaccataac tggcagggta tgcttatttc cgcattttcc gatgagggat
    18901 aaagatgagt aaaccacctc ttttctttat tgttatcatt ggcttaatca tcgtcgccgc
    18961 atcgtttcgt tttatgcaac aacgacggga aaaagctgat aatgatatgg caccgctcca
    19021 gcaaaagctg gtggtggtga gcaacaagcg ggaaaaaccg cttaacgatc gccgttcgcg
    19081 ccagcaggaa gtgactccgg caggcaccag tatgcgctat gaggcaagct tcaaaccgca
    19141 aagcggagga atggagcaga cgtttcgcct cgacgcccag cagtaccacg ccctgacagt
    19201 gggtgataaa ggtacgctga gctataaagg aacgcgcttt gtcagctttg taggcgaaca
    19261 ataaggttat tttttaactt taaatttctt ctgccagacc agcagttcaa acacgccgaa
    19321 aaggaaaatc cgcacctttt cggcggtggt catctgcggg ccatctttcg gtaaagtgga
    19381 tttcaatagc gccagctgca taccatgcat cagcacggta aaaatcagcg ccacgttaac
    19441 gaaaatattc agcgggcgtg ggaagggatg caccaggttg aggattaaaa atccccaaac
    19501 acagagcatt aacaaacgac caatattaat cagcatcact ttctccttgt gcttcgcgtt
    19561 gatacagccg ataagccacc tgacccgcca ctttttcccg atgtaatgac cagtttgccg
    19621 gaacagtggg cagacccttt tcgacttcgc tttcgacata aatcagggct tcgtcagcca
    19681 gccagccgtt atcttccagt aaatttatcg tctcttctaa caagccacgg cggaacggtg
    19741 gatcgacaaa cacgatatta tgcggtgtgc ctttttgcgc caggaatgac atcgcgttgc
    19801 tgttcaccac gcgtgcattg cctgctttta gtgtcgccag attcttaatt aactgctgag
    19861 aaaccgcacg atccatctca atcaacgtgg cccccgcagc gtagcgcgat aacgcttcca
    19921 gccccagcgc gccgctcccg gcgaagcaat ccagacattg ggcgtcaaca atgaccggag
    19981 ccagccagtt aaacaacgtt tcgcgtacgc ggtcggtggt ggggcgcaga cctggactat
    20041 caggaaccgg gagtttacgg cctcgccact gcccgccaat aatgcggatt tggccgctgc
    20101 cggaatgatt cggttttttc atgataaatt gctcaatccg ccagataaca catacttgca
    20161 ggcggtgatg tgaattaagt taagtgatag actatttcat cattttttta gctgctatgt
    20221 acatagcgtt aacgctgtgc catgaagcaa cagcgaggag tgtagtcgca aatggcgaaa
    20281 gaaaaaaaac gtggcttttt ttcctggctg ggctttggtc aaaaagagca gaccccggaa
    20341 aaagagacag aagttcagaa tgaacaacca gttgtagaag aaatcgttca ggcgcaagag
    20401 cctgtgaagg cttctgaaca cgccgttgaa gagcagccgc aggcgcatac tgaagccgag
    20461 gcggaaactt ttgctgccga cgttgtggaa gttactgaac aggttgctga aagtgaaaaa
    20521 gcgcagcctg aagcggaagt cgttgcacag ccggaaccgg tcgtagaaga aacgccggag
    20581 ccagtggcta tcgaacgtga agaactgccg ttgtcggaag acgtcaacgc cgaagcggtt
    20641 tcgccagaag agtggcaggc cgaagcggaa accgtagaga ttgtcgaagc ggcagaagag
    20701 gcggtaatgg tggtttctcc ggcagaagaa gagcagccgg tggaagaaat cgctcaggag
    20761 caggaaaaac cgaccaaaga aggtttcttc gcgcgcctga aacgcagcct gttaaaaacc
    20821 aaagaaaatc tcggttccgg atttatcagc ctgttccgcg gtaaaaaaat cgacgatgat
    20881 ctgtttgaag agctggaaga acagctgttg atcgccgatg tgggcgtgga aaccactcgt
    20941 aaaattatca ccaatctgac ggaaggcgca tcccgcaagc agcttcgtga tgccgaggcg
    21001 ctctatggcc tgctgaaaga agagatgggc gagattctgg cgaaagtcga tgagccgctg
    21061 aatgttgaag gtaaaacgcc gttcgtgatc ctgatggtgg gcgtcaacgg tgtgggtaaa
    21121 accacgacga ttggtaagct ggcgcgtcag tttgagcagc agggtaaatc ggtgatgctg
    21181 gcggcaggcg atactttccg tgcggcagcg gttgaacagc ttcaggtctg gggtcagcgc
    21241 aacaatattc cggtgattgc ccagcatacc ggtgcggatt ccgcttctgt tatcttcgat
    21301 gccattcagg ccgctaaagc gcgtaacatc gacgtcctga ttgccgatac cgccggacgc
    21361 ctgcaaaaca aatcgcacct gatggaagag ttgaagaaaa tcgtccgcgt gatgaagaaa
    21421 ctcgacgttg aagctccgca tgaagttatg ctgaccatcg atgccagcac cgggcagaat
    21481 gcggtaagcc aggccaaact gttccacgaa gccgttggct taaccggcat cacgctaacg
    21541 aaactggacg gcacggcgaa aggcggggta attttctcgg tggctgacca gtttggtatc
    21601 cctatccgct acattggtgt cggcgaacgt attgaggatt tgcgtccgtt taaggcggac
    21661 gactttatag aggcactttt tgcccgagag gattaacaat gattcgcttt gaacatgtca
    21721 gcaaggctta tctcggtggg agacaggcgc tgcagggcgt tacgttccat atgcagccgg
    21781 gtgagatggc gtttctgacc ggtcattccg gcgcagggaa aagtaccctc ctgaagctga
    21841 tctgtgggat tgagcgtccc agcgccggga aaatctggtt tagcggccat gacatcacgc
    21901 gtctgaaaaa ccgtgaagtt ccgtttctgc gccgccagat tggcatgatt ttccaggatc
    21961 accatctact gatggaccgt actgtctatg ataacgtggc gatcccgctg attatcgccg
    22021 gtgccagcgg tgatgacatt cgtcgccggg tgtcggcggc gctggataaa gtcgggctac
    22081 tggacaaagc gaagaacttc cctattcagc tttccggcgg tgaacaacag cgtgttggca
    22141 ttgcccgcgc ggtggtgaac aagcccgcgg tactgctggc ggacgaaccg actggtaacc
    22201 tggacgacgc gctgtcggaa ggcattttac gtctgtttga agagtttaac cgcgttgggg
    22261 taaccgtatt gatggcaacg cacgacatca acctgatttc gcgacgttcc tatcgcatgc
    22321 tcaccctgag cgatggtcac ttgcatggag gcgtgggcca tgaataagcg cgatgcaatc
    22381 aatcacattc ggcagtttgg cgggcgtctg gatcgcttcc gtaaatcggt cggcggctct
    22441 ggcgacggcg gtcgtaacgc gccgaaacgc gcgaaatcct cgccaaaacc ggtaaatcgc
    22501 aaaaccaacg ttttcaacga acaggtacgc tatgccttcc acggcgcatt gcaggatctg
    22561 aaaagcaaac cgttcgccac gtttttaacg gtgatggtta tcgccatctc cctgacgctg
    22621 cccagcgtct gttatatggt gtacaaaaac gttaaccagg cggcgacgca atattatccg
    22681 tcaccgcaaa tcactgttta tctgcaaaaa acgctggatg atgacgccgc tgcgggcgtg
    22741 gtggcacagt tgcaggccga gcaaggcgtg gagaaagtga attatctttc tcgtgaagac
    22801 gcactgggcg agttccgtaa ctggtctggt tttggtggtg cgctggatat gctggaagaa
    22861 aacccgcttc cggcagtagc ggtggtgatc ccgaaactcg attttcaggg gacggagtca
    22921 ctgaatacgc tgcgtgatcg tatcacgcag attaacggca ttgacgaagt gcggatggat
    22981 gacagctggt ttgcccgcct ggcggcgttg accgggctgg tcgggcgcgt ttcggcaatg
    23041 atcggcgtat tgatggtggc ggcagtgttc ctcgtcatcg gtaacagtgt gcgcctgagt
    23101 atctttgctc gccgtgattc tattaacgtg cagaaactga ttggtgcgac ggatggtttc
    23161 atcctgcgtc cgttcctgta cggtggcgca ttgctgggat tttctggcgc attgttgtca
    23221 ctaattttgt cagaaattct ggtgctgcgt ttgtcatcgg cggttgcgga agtggcacag
    23281 gttttcggaa cgaagtttga tatcaatggc ttatcgttcg atgaatgcct gctattgctg
    23341 ctggtatgct cgatgattgg ctgggtggca gcgtggcttg ccacggtaca acatttacgc
    23401 cactttacgc ctgaataata aaagcgtgtt atactctttc cctgcaatgg gttccgtagc
    23461 agggaaagag accccgttgt ctcttcccgg tattttatct ctatgtcaca ttttgtgcgt
    23521 aatttattca caagcttgca ttgaacttgt ggataaaatc acggtctgat aaaacagtga
    23581 atgataacct cgttgctctt aagctctggc acagttgttg ctaccactga agcgccagaa
    23641 gatatcgatt gagaggattt gaatgactga caaaatgcaa agtttagctt tagccccagt
    23701 tggcaacctg gattcctaca tccgggcagc taacgcgtgg ccgatgttgt cggctgacga
    23761 ggagcgggcg ctggctgaaa agctgcatta ccatggcgat ctggaagcag ctaaaacgct
    23821 gatcctgtct cacctgcggt ttgttgttca tattgctcgt aattatgcgg gctatggcct
    23881 gccacaggcg gatttgattc aggaaggtaa catcggcctg atgaaagcag tgcgccgttt
    23941 taacccggaa gtgggtgtgc gcctggtctc cttcgccgtt cactggatca aagcagagat
    24001 ccacgaatac gttctgcgta actggcgtat cgtcaaagtt gcgaccacca aagcgcaacg
    24061 caaactgttc ttcaacctgc gtaaaaccaa gcagcgtctg ggctggttta accaggatga
    24121 agtcgagatg gtggcccgtg aactgggcgt aaccagcaaa gacgtacgtg agatggaatc
    24181 acgtatggcg gcacaggaca tgacctttga cctgtcttcc gacgacgatt ccgacagcca
    24241 gccgatggca ccggtgctct atctgcagga taaatcatct aactttgccg atggcattga
    24301 agatgataac tgggaagagc aggcggcaaa ccgcctgacc gatgcgatgc aaggtctgga
    24361 cgagcgtagc caggatatca tccgcgcgcg ctggctggat gaagacaaca agtccacgtt
    24421 gcaggaactg gctgaccgtt acggtgtttc cgctgagcgt gtgcgtcagc ttgaaaagaa
    24481 cgcgatgaaa aaattgcgcg ctgctattga agcgtaattt ccgctattaa gcagagaacc
    24541 ctggatgaga gtccggggtt tttgtttttt gggcctctac aataatcaat tccccctccg
    24601 gcaaaacgcc aatccccacg cagattgtta ataaactgtc aaaatagcta ttccaatatc
    24661 ataaaaatgg ggtatgtttt agcagagtat gctgctaaag cacgggtagc tacgtataaa
    24721 acgaaataaa gtgctgcaca acaacatcac aacacacgta ataaccagaa gagtggggat
    24781 tctcaggatg aacataaagg gtaaagcgtt actggcagga tgtatcgcgc tggcattcag
    24841 caatatggct ctggcagaag atattaaagt cgccgtcgta ggtgcaatgt ccggtccggt
    24901 ggcgcagtac ggtgaccagg agtttactgg cgcagagcag gcggttgcgg atatcaatgc
    24961 taaaggtggt atcaaaggca acaaactgca aatcgtaaaa tatgacgatg cctgtgaccc
    25021 gaaacaggcg gttgcggtgg cgaacaaagt cgttaacgac ggcattaaat acgtgattgg
    25081 tcacctctgc tcttcatcaa cgcagccagc gtctgacatc tacgaagacg aaggcatttt
    25141 aatgatcacc ccagcggcaa ccgcaccaga gctgaccgcc cgtggctatc agctgatcct
    25201 gcgcaccacc ggcctggact ccgaccaggg gccgacggcg gcgaaatata ttcttgagaa
    25261 agtgaaaccg caacgcattg ctatcgttca cgacaaacag cagtacggcg aaggtctggc
    25321 gcgtgcagtg caggacggcc tgaagaaagg caatgctaac gtggtgttct ttgatggcat
    25381 caccgccggg gaaaaagatt tctctaccct ggtggcgcgt ttgaagaaag agaatatcga
    25441 tttcgtttac tacggcggtt atcacccgga aatggggcaa atcctgcgtc aggcacgcgc
    25501 ggcagggctg aaaactcagt ttatggggcc agaaggcgtg gctaacgtct cgctgtctaa
    25561 cattgcgggc gaatcagcgg aagggctgct ggtgaccaag ccgaagaact acgatcaggt
    25621 tccggcgaac aaacccattg ttgacgcgat caaagcgaaa aaacaggacc caagcggcgc
    25681 gttcgtctgg accacctacg ccgcgctgca atctttgcaa gcggggctga atcagtctga
    25741 cgatccggcg gagatcgcta aatacctgaa agcgaactcg gttgatacgg tgatggggcc
    25801 gctgacctgg gatgagaaag gcgatctgaa aggctttgag ttcggtgtgt ttgactggca
    25861 cgccaacggc acggcgactg atgcgaagta atcattaatc ggcaactttg ggttgccgcc
    25921 aaattgctaa tatcgagtac gttgcttcat gccggatgcg gcgtaaacgc cttatccggc
    25981 ctacaagatt caaagaaatc agtaaattgc agcacacatt gtaggcctga taagcgtagc
    26041 gcatcaggca atatcacttt tgaaatcaaa cttgacgatt aatgcttctc ccagccaccc
    26101 tgttgtgccg taaatcccag tgctcgcata aacgctgtca tcacgccgcg atcttccacg
    26161 ccagcatccg ccatccacca acatgaaacg ccaggattgt tacgcaaaac ctcttccagc
    26221 agatattgcc ccacaccgcg acgtcgggtg acttcccgca cgcgtagaga atccagtgcc
    26281 ccctcggtgc cgcttaaggt tacccgcacg gcagcgagca ggcgctcgtt aaaacgcgcg
    26341 gcgtagatgc ggtggttatc gtcaacctgt aaagaggaag gggaatattc cggccagatc
    26401 ttttgcaggt caatccggtc ctggtcgcta aagttttcta atcgaatgat ggtcagcttc
    26461 atggggaacc cgtgtaaatc acaaaagtgg agccagtgta gcgaaataat ttaatcggag
    26521 gctttctctt ttttatttct tttggcaggt gattaatttt ttttcagcaa taattacaaa
    26581 gttaaaacat tagagaatga aaaatatcca gcataatccc ctaaatggta gtgaattatt
    26641 ctgccctttg taccgttatt ttatgctgac aacggcgctt ttttctgttt atctatcaat
    26701 aaattcagaa tattatctgt tcttaatcga ctgaaaaata gggattttaa tcgctatcat
    26761 cacaaaatac tgcgctaaac ccttaatcag acaggtaaaa acagtgcagt ataaaaaaga
    26821 acagtctgat ttgttaacac ataagaacaa agcaacacaa catcacgaat ggggattttt
    26881 gactatgaaa cggaatgcga aaacgatcat cgcagggatg attgcactga caatttcaca
    26941 caccgctatg gctgacgata ttaaagtcgc cgttgtcggc gcgatgtccg gcccgattgc
    27001 ccagtggggc gatatggaat ttaacggcgc gcgtcaggca attaaagaca ttaatgccaa
    27061 agggggaatt aagggcgata aactggttgg cgtggaatat gacgacgcct gcgacccgaa
    27121 acaagccgtt gcggtcgcca acaaaatcgt taacgacggc attaaatatg ttattggtca
    27181 tctgtgttcc tcctctaccc aacctgcatc agatatctat gaagacgaag gtattctgat
    27241 gatctcgccg ggagcgacca atccggagct gacccaacgc ggttatcaat acatcatgcg
    27301 tactgccggg ctggactctt cccaggggcc aacggcggca aaatacattc ttgagacggt
    27361 gaagccccag cgcatcgcca tcattcacga caaacaacag tatggcgaag ggctggcacg
    27421 ttcggtgcag gacgggctga aagcggctaa cgccaacgtc gtcttcttcg acggcattac
    27481 cgctggagag aaagatttct ccgcgctgat cgcccgcctg aaaaaagaaa acatcgactt
    27541 cgtttactac ggcggttact acccggaaat ggggcagatg ctgcgccagg cccgttccgt
    27601 tggcctgaaa acccagttta tggggccgga aggtgtcggt aatgcgtcac tgtcgaacat
    27661 tgccggtgat gccgctgaag gcatgttggt caccatgcca aaacgctatg accaggatcc
    27721 gacaaaccag ggcatcgttg atgcgctgaa agccgacaag aaagatccgt ccgggcccta
    27781 tgtctggatc acctacgcgg cggtgcaatc tctggcaact gcccttgaac gtaccggcag
    27841 cgatgagccg ctggcgctgg tgaaagattt aaaagctaac ggtgcaaaca ccgtaattgg
    27901 gccgctgaac tgggatgaaa aaggcgatct taagggattc gattttggtg tcttccagtg
    27961 gcacgccgac ggttcatcca cggcagccaa gtgatcatcc taccgtccgt gaaaaacggg
    28021 cgggtttaga aaggttacct tatgtctgag cagtttttgt atttcttgca gcagatgttt
    28081 aacggcgtca cgctgggcag tacctacgcg ctgatagcca tcggctacac catggtttac
    28141 ggcattatcg gcatgatcaa cttcgcccac ggcgaggttt acatgattgg cagctacgtc
    28201 tcctttatga tcatcgccgc actgatgatg ctgggcattg ataccggctg gctgctggtg
    28261 gcggcgggat tcgtcggcgc aatcgtcatt gccagcgcct acggctggag tatcgaacgg
    28321 gtagcctatc gcccggtgcg taactctaag cgcctgattg cactcatctc ggcaatcggc
    28381 atgtctatct tcctgcaaaa ctacgtcagc ctgaccgaag gttcgcgcga tgtggcgctg
    28441 cccagcctgt ttaacggtca gtgggtggtg ggccatagcg aaaacttctc tgcgtctatt
    28501 accaccatgc aggcggtgat atggattgtt actttcctcg ccatgctggc gctgacgatt
    28561 ttcattcgct attcccgtat ggggcgcgcc tgccgtgcct gcgcggaaga tctgaaaatg
    28621 gcgagcctgc ttggcatcaa caccgaccgg gtgattgcgc tgacctttgt gattggcgcg
    28681 gcgatggcgg cagtggcggg cgtgctgctc ggtcagttct acggcgtaat taacccttac
    28741 atcggcttta tggccgggat gaaagccttt accgctgcgg tgctcggtgg gattggcagc
    28801 attccggggg cgatgattgg cgggctgatc ctggggattg cggaggcgct ctcttctgcc
    28861 tatctgagta cggaatataa agatgtggtc tcattcgccc tgctgatcct ggtgctgctg
    28921 gtgatgccga ccggtattct gggtcgcccg gaggtagaga aagtatgaaa ccgatgcata
    28981 ttgcaatggc gctgctctct gccgctatgt tctttgtgct ggcgggcgtc tttatgggcg
    29041 tgcaactgga gctggatggc accaaactgg tggtcgacac ggcttcggag atccgttggc
    29101 agtgggtgtt tatcggcacg gcggtggtct ttttcttcca gcttttgcga ccggctttcc
    29161 agaaagggct aaaaagcgtt tccgggccga agtttattct gcccgcgatt gatggctcca
    29221 cggtgaagca gaaactgttc ctcgtggcgc tgctggtgct tgcggtggcg tggccattta
    29281 tggtgtcgcg tgggacggtg gatatcgcca ctctgaccat gatctacatt attctcggtc
    29341 tcgggctgaa cgtggttgtt ggtctttctg gtctgctggt gctgggatac ggcggctttt
    29401 acgccatcgg cgcttacact tttgcgctgc tcaatcacta ttacggtctg ggcttctgga
    29461 cctgtctgcc gattgccggg ttaatggcgg cggcggcggg cttcctgctc ggttttccgg
    29521 tgctgcgtct gcgcggtgac tatctggcga tcgtgaccct cggtttcgga gaaattgtgc
    29581 gcatattgct gctcaataac actgaaatca ccggcggtcc gaacggaatc agtcagatcc
    29641 ccaaaccgac actcttcgga ctcgagttca gtcgtaccgc ccgcgaaggc ggctgggaca
    29701 cgttcagcaa tttctttggc ctgaaatacg atccctccga tcgcgtcatt ttcctctatc
    29761 tggtggcgtt gctgttggtg gtgttaagcc tgtttgtcat taaccgcctg ctgcggatgc
    29821 cgctggggcg tgcgtgggaa gcgttgcgtg aagatgaaat cgcttgtcgt tcgctgggct
    29881 taagcccgcg tcgtatcaag ctgactgcct ttaccatcag cgctgcgttt gccggttttg
    29941 ccggaacgtt gtttgctgcg cgccagggct ttgtcagccc tgaatccttc acttttgccg
    30001 aatcggcctt tgtgctggcg atagtggtgc tcggcggtat gggctcgcag tttgcagtga
    30061 ttctggcggc tgttttgctg gtggtctcgc gcgagttgat gcgtgatttc aacgaataca
    30121 gcatgttaat gctcggtggt ttgatggtgc tgatgatgat ctggcgtccg cagggcttgc
    30181 tgcccatgac gcgcccgcaa ctcaagctga aaaacggcgc agcaaaagga gagcagtcat
    30241 gagtcagcca ttattatctg ttaacggcct gatgatgcgc ttcggcggcc tgctggcggt
    30301 gaacaacgtc aatcttgaac tgtacccgca ggagatcgtc tcgttaatcg gccctaacgg
    30361 tgccggaaaa accacggtct tcaactgcct gaccggattc tataaaccca ccggcggcac
    30421 cattttactg cgcgatcagc acctggaagg tttaccgggg cagcaaattg cccgcatggg
    30481 cgtggtgcgc accttccagc atgtacgtct gttccgtgaa atgacggtga ttgaaaacct
    30541 gctggtggca cagcatcagc aactgaaaac tgggctgttc tccggcctgt tgaaaacccc
    30601 ctctttccgt cgcgcccaga gcgaagcgct cgaccgcgcc gccacctggc ttgagcgcat
    30661 tggtttgctg gaacatgcca accgtcaggc gagtaacctg gcctatggtg accagcgccg
    30721 tctggagatt gcccgctgta tggtgacgca gccggagatt ttaatgctcg acgaaccggc
    30781 agcaggtctt aacccgaaag agacgaaaga gctggatgag ctgattgccg aactgcgtaa
    30841 ccatcacaac accactatct tattgattga acacgatatg aagctggtga tggggatttc
    30901 agacagaatt tacgtggtca atcaggggac gccgctggca aacggtacac cggagcagat
    30961 ccgtaataac ccggacgtga tccgtgccta tttaggtgag gcataagatg gaaaaagtca
    31021 tgttgtcctt tgacaaagtc agcgcccact acggcaaaat ccaggcgctg catgaggtga
    31081 gcctgcatat caatcagggc gagattgtca ccctcattgg cgcgaacggg gcggggaaaa
    31141 ccaccttgct cggcacgtta tgcggcgatc cgcgcgccac cagcgggcga attgtgtttg
    31201 atgataaaga cattaccgac tggcagacgg cgaaaatcat gcgcgaagcg gtagcgattg
    31261 tcccggaagg acgtcgcgtc ttctcgcgga tgacggtgga agagaacctg gcgatgggcg
    31321 gcttttttgc cgaacgcgac cagttccagg agcgcataaa gtgggtctat gaactgtttc
    31381 cacgtctgca tgagcgccgt gttcagcgag cgggcactat gtctggcggt gaacagcaga
    31441 tgctggcgat tggtcgcgcg ctgatgagca acccgcgttt gttgttgctt gatgagccat
    31501 cgctcgggct ggcaccgatt atcatccagc aaattttcga caccatcgaa caactgcgcg
    31561 agcaggggat gaccatcttc cttgtcgagc agaacgccaa ccaggcgcta aagctggcgg
    31621 atcgcggcta cgtgctggaa aacggtcatg tagtgctctc cgataccggc gacgcgctgc
    31681 tggcgaatga agcggtgaga agtgcgtatt taggcgggta ataatatttt gagtaaaaag
    31741 gagtcgaatg actccttttt tgtacttgtt agttgtacaa tttaattgta cttaatatgg
    31801 aggatatcat gcatactatg aactacagcg aagcgcggca gaaccttgca ttggttctgg
    31861 aaacagcggc gacaggtact ccggtaacga tcacgaggcg tgggcataag gcagctgtga
    31921 ttattagcgc agaagagttt gaacgttacc agaaggctaa actggacgca gaatttgacg
    31981 ccatcatggc tattcatggc agcgaaatta aggagttagc ggataaatga ccattcagtt
    32041 tatctcagcg gaggaaataa tttatttcca cgataaattg cttagcgtta caccaggtgt
    32101 aacaggaatg tccgatctag ggcgagcaga agcgcttttg taccgggtac tgaataagta
    32161 tgagtatgaa ggtgtttctg atctttggct gctggcggcg atgcatttat tggcaatctc
    32221 gcgcggtcat atctttaatg atggtaataa gcgcaccgca ctgtttatca cgctattatg
    32281 ccttaaacgt aacggcatca tacttgctgc aaacccagag tttgtagaat tgacggttga
    32341 tgtggcggcg ggaaaagtat cgttagaaga tatcgttacc cgcttgcgtc aacgcggtac
    32401 ctgacaaggt tcttagccat tctgcttttt atccgtgccc catctcccat tttccctcac
    32461 ccgtaccgtc accgccttgt catctttctg acaccttact atcttacaaa tgtaacaaaa
    32521 aagttatttt tctgtaattc gagcatgtca tgttaccccg cgagcataaa acgcgtgaat
    32581 tcgcgcattc ggtacaacaa gagagataaa cgatgaaacc gttacattat acagcttcag
    32641 cactggcgct cggactggcg ttaatgggga atgcacaggc agtgacgacc attccgttct
    32701 ggcattctat ggaaggggaa ctgggtaaag aggtggattc tctggcccaa cgttttaacg
    32761 ccgaaaaccc ggattacaaa attgtaccga cctataaagg caactacgaa cagaatttaa
    32821 gcgcggggat tgccgcattt cgtaccggca acgctccggc tattttgcag gtttatgaag
    32881 ttggcaccgc caccatgatg gcgtcgaaag ccattaaacc ggtgtatgac gtatttaaag
    32941 aggcggggat tcaattcgat gagtcgcagt ttgtgccgac ggtttcaggc tattactccg
    33001 acagcaaaac cggccactta ctctcccagc cgttcaacag ctcgactccc gttctctatt
    33061 acaacaaaga cgccttcaag aaagcaggat tagacccgga acagccgccg aaaacctggc
    33121 aggatctggc ggactatgcc gcaaaactaa aagcctccgg catgaagtgc ggctacgcca
    33181 gcggctggca gggctggatc caactggaaa actttagcgc ctggaacggt ctgccgtttg
    33241 ccagcaaaaa caacggcttt gacggcacag acgcggtgct tgagttcaac aagccggagc
    33301 aggtgaaaca catcgccatg ctcgaagaga tgaacaagaa gggcgatttc agctacgttg
    33361 ggcgtaagga tgaatccacc gagaagttct ataacggtga ttgcgcgatg actaccgcct
    33421 cttccggttc acttgccaac attcgcgagt acgccaaatt taactatggc gtaggcatga
    33481 tgccttacga cgccgatgcg aaagacgcgc cgcaaaacgc cattatcggc ggagccagtc
    33541 tatgggtaat gcagggtaaa gataaagaaa cctacaccgg cgtggcgaag ttcctcgact
    33601 tcctcgcaaa gccagaaaac gctgccgagt ggcatcagaa aaccggctat ctgccaatca
    33661 ctaaagcggc gtatgacctg acccgtgagc agggctttta cgagaaaaac ccaggagcgg
    33721 atattgccac gcgtcagatg ctgaacaagc caccgttgcc gttcaccaaa ggtttgcgtc
    33781 tgggcaacat gccgcagatc cgcgtgattg tggatgaaga gctggagagt gtgtggaccg
    33841 gtaagaagac accacagcag gcgctggata ctgccgttga gcgtgggaac cagttactgc
    33901 gccgctttga gaaatcgacg aagtcttaat cagtgtaatg tcggatgcgt ttcgcttatc
    33961 tgacctggca tcgcgtgtag gccggataag cgaagcgcat ccggcacagt tcaggaatta
    34021 actgtaatgt catcatcccg tccggtgttc cgctcgcgct ggctgcctta tttgctggtc
    34081 gcgccgcagc tcatcatcac cgttatcttt tttatctggc ctgcgggcga agcgttgtgg
    34141 tactcgctac aaagcgtcga tccgtttggt ttctccagcc agtttgtcgg cctggataat
    34201 ttcgtcacgc tgtttcacga cagctactat ctcgacgcct tctggacgac gataaaattc
    34261 agcacctttg tcaccgtcag cggtttgctg gtatcgctgt tcttcgcagc gctggtggag
    34321 tacatcgtgc gcggcagtcg tttctatcaa actttgatgc tgctgccgta tgccgtggct
    34381 cccgccgttg ccgccgtatt gtggatcttt ctgtttaacc ctggtcgcgg gctgatcacc
    34441 cattttctcg ctgagttcgg ctacgactgg aaccacgcgc aaaacagcgg tcaggcgatg
    34501 ttcctggtgg tgtttgcctc agtgtggaag caaatcagct acaactttct gttcttctat
    34561 gccgcgctgc aatccatccc ccgttcgttg atcgaagccg cagccattga tggggctggc
    34621 ccgattcgcc gcttctttaa gattgcgctg ccgcttatcg ctccggtgag tttcttcctg
    34681 ctggtggtga acctggtgta tgccttcttc gacaccttcc cggtgatcga cgccgccacg
    34741 tccggcgggc cagtacaggc caccacgacg ctgatttata agatttaccg tgaaggcttt
    34801 accggactgg atttggcttc gtctgccgcg cagtcggtgg tgttgatgtt cctcgtcatc
    34861 gtgctgacgg tggtgcagtt ccgctatgtt gaaagcaagg tacgttacca atgattgaga
    34921 accgtccgtg gctgacaata ttcagccata ccatgctgat cctcgggatc gcggtgatcc
    34981 tcttcccgct gtacgtggcg tttgtcgcgg cgacgctgga taaacaagaa gtctatgccg
    35041 caccgatgac gctaatcccc ggcacacatc tgctggaaaa catccacaac atctgggtaa
    35101 acggggtagg cacgaatagc gcgccgttct ggcggatgtt gcttaacagc tttgtgatgg
    35161 cgttcagcat tacgctcggc aaaattaccg tctcgatgct ctcggcattt gccattgtct
    35221 ggtttcgttt tccgctacgt aacctcttct tctggatgat ttttatcacc ctgatgttgc
    35281 cggttgaagt gcgtatcttc ccgacggtgg aagtcatcgc caacctgaaa atgctcgaca
    35341 gctacgcagg tttaacgctg ccgctgatgg cctcggcgac cgccacgttt ctgtttcgcc
    35401 agttctttat gaccctaccg gatgaactgg tggaagccgc gcggatcgac ggcgcgtcgc
    35461 caatgcgttt cttttgcgac atcgtttttc cgctgtccaa aactaatctg gcggcgctgt
    35521 ttgtgatcac ctttatttac ggctggaacc agtatttgtg gccgttgttg attattaccg
    35581 acgttgatct cggcaccacc gtggcaggga ttaaagggat gatcgccaca ggcgaaggca
    35641 caacggaatg gaactcggtg atggcggcga tgttgttaac cctgatccca ccagtagtga
    35701 ttgttttagt gatgcagcgt gccttcgtgc gcggcctggt cgatagtgag aaataagatg
    35761 gcaggactga aattacaggc cgtaaccaaa agctgggatg gcaaaaccca ggtcattaaa
    35821 ccgctgaccc ttgatgtggc ggatggcgaa tttatcgtga tggtcgggcc gtctggctgc
    35881 gggaaatcga cgttattgcg aatggttgcc ggactggagc gggtaacgac gggcgatatc
    35941 tggatcgacc gcaagcgtgt caccgaaatg gagccgaaag atcgcgggat tgcgatggtg
    36001 ttccagaact acgcgctcta tccgcatatg agcgtagagg agaacatggc gtgggggctg
    36061 aaaattcgcg gcatgggcaa gcaacaaatt gccgaacgtg ttaaagaagc ggcgcgcatt
    36121 ctggagctgg acggcctgct taagcgccgc ccgcgtgagc tatctggcgg tcagcgtcag
    36181 cgtgtggcga tggggcgtgc gattgtgcgt gagccggcag tgttcctgtt tgatgagccg
    36241 ctctctaacc tcgatgccaa gttgcgtgtg cagatgcgtc ttgaactgca acagctgcac
    36301 cgtcgcctga aaacgacttc actctacgtt acccacgatc aggttgaggc gatgacgctc
    36361 gcccagcgag taatggtgat gaacggcggc gtcgccgaac agattggcac gccagttgaa
    36421 gtgtacgaaa agcccgccag cctgtttgtg gcgagtttta tcggcagccc ggcgatgaac
    36481 ctactggctg gccgcgtgaa taacgaaggt acgcatttcg aactggatgg tggtattacg
    36541 ctgccgctaa acggtggcta ccgtcagtat gccgggcgta aaatgaccct cggcattcgc
    36601 ccggaacata tagcgctaag ctcgcaggca gaaggtggtg tgccgctggt gatggacacg
    36661 ctggagatcc tcggcgcaga taacctggcg cacggacgtt ggggcgaaca gaagctggtg
    36721 gtgcgactgg cacatcagga gcgcccgacg gcaggcagca cgctgtggct gcatctgccg
    36781 gaaaatcagc tgcatctttt tgatggtgaa acaggacaac gagtatgagt aactggcctt
    36841 atccccgcat cgtcgcccat cgtggtggcg gtaagctggc cccggagaac accctggcgg
    36901 caatcgacgt cggggcaaaa tacggtcata agatgatcga atttgacgcg aagttatcga
    36961 aagatggcga gatctttctg ctccatgacg acaatctcga acgtaccagc aacggttggg
    37021 gcgttgcggg cgagctgaac tggcaggatt tactgcgcgt ggatgcaggc agttggtaca
    37081 gcaaagcgtt taaaggtgag ccgctgccgt tgctttcgca ggtggcggaa cgctgccgcg
    37141 aacacgggat gatggcgaat atcgaaatca aacccaccac tggcactgga ccattaacgg
    37201 gcaaaacggt ggcgttggcg gcacgcgaac tgtggaccgg tatgacgccg ccgctgctgt
    37261 catcgtttga gattgatgct ttagaagccg cgcaacaggc agcaccggaa ctgccgcgtg
    37321 gtttattgct tgatgaatgg cgcgacgact ggcgagaatt gaccgcgcga ctgggctgcg
    37381 tctctattca tctcaatcat aagttgctcg ataaagcgcg agtgatgcag ttgaaagatg
    37441 ccggattacg gattctggtt tataccgtca ataaacccca gcgcgcggca gagttgctgc
    37501 gctggggcgt ggattgcatc tgtaccgatg cgattgacgt gattggtccg aactttacgg
    37561 cccaatagtt ttcaacggaa tgtcaggctg cggcgtgctg ggctgattca gcatgtcgcc
    37621 gtttctcctc tccggcagca tatgctgctg attcaacgaa ctatccggat tacggttgct
    37681 gtttaacatc ccgccgttgg tattgggcag catttgctgc cgggcgggat ttcgctcccc
    37741 cggctgtgac tgcaacaccc gctgagaatt attgtttatc tggttttcta aatgctgctg
    37801 ttgcagctgc gtttgcgttt tcagttgctg gttcagcatc cctttttgct ggatttgctg
    37861 cgtctgcatt tgagtttgca tccgctgctg gctggggatc tgatatcccg gctggttagg
    37921 gttgttcaga gtattaatgg gctgtgcaaa gccgacaaac ggcaggagtg ccgtcaaaag
    37981 cagaagtcgt ttcatcgcgt atcctcctct gaagatattc tttaagttta ctcgcttccc
    38041 ggcaaaacga tgattaattc agagttatat accaggctta gctggggttg ccccttaatc
    38101 tctggagaat aacgatgata aaaccgacgt ttttacgccg ggtggccatt gctgctctgc
    38161 tcacaggaag ttgttttagt acggtagccg cgccacctgt ttcttatggt gtggaggaag
    38221 atgtcttcca tccggtacgc gctaagcagg gaatggtggc gtctgtagat gctactgcca
    38281 ctcaggtggg ggtggatatc ctcaaggagg gcgggaatgc cgttgatgcc gccgtggcgg
    38341 tgggctacgc gctggcagta acgcatccgc aggcagggaa tctgggcggc ggtggtttta
    38401 tgctgatccg ctcgaaaaat ggcaatacca cggctatcga tttccgtgaa atggcacccg
    38461 ccaaagcgac ccgcgatatg ttcctcgatg atcagggcaa cccggacagc aaaaaatcac
    38521 tcacttcgca tctggcttcc ggcacacccg gtacggtagc gggtttctcg ttggcgctgg
    38581 ataaatacgg caccatgccg ttgaataaag tggtgcaacc ggcgtttaaa ctggcacgcg
    38641 atggttttat cgttaacgac gcgctggctg acgatctcaa aacctacggc agtgaagtgc
    38701 tgccgaacca cgaaaacagc aaagctatct tctggaaaga gggcgaaccg ctgaaaaagg
    38761 gcgacaagct ggtgcaggcg aacctggcaa agagcctgga gatgattgcc gaaaacggcc
    38821 cggatgaatt ctacaaaggc acgatagcgg aacagattgc ccaggagatg cagaaaaacg
    38881 gtggcttgat cagcaaagaa gatttagcgg catataaagc ggtcgaacgc acaccgataa
    38941 gcggcgatta tcgcgggtat caggtttact ccatgccacc gccatcttcc ggcggtattc
    39001 atatcgtaca aatcctcaat attctggaaa acttcgatat gcagaagtac ggctttggca
    39061 gcgccgacgc gatgcaaatc atggcagaag ctgagaaata cgcctatgcc gaccgctcgg
    39121 aatatcttgg cgacccggat tttgtcaaag taccgtggca ggcgctgacc aataaagcct
    39181 atgccaaatc tattgccgat caaatcgata tcaataaagc gaagccatcc agcgaaattc
    39241 gtcctggcaa gcttgcgcct tatgagagta atcaaactac ccattactca gtagtggata
    39301 aagacggtaa cgccgtggcg gtgacctata cgctgaatac caccttcggt acgggcattg
    39361 tcgcgggcga gagcggtatt ctgcttaata accaaatgga tgatttctcc gccaaaccag
    39421 gcgtaccgaa cgtttacggg ctggtgggcg gtgatgccaa cgccgtcggg ccgaataaac
    39481 gcccgctgtc gtcgatgtca ccaaccattg tggtcaagga tggtaaaacc tggctggtca
    39541 ccggaagccc aggcggtagc cggattatca ccactgtgct gcaaatggtg gtgaatagca
    39601 tcgattatgg aatgaacgtc gccgaagcga ccaatgcgcc gcgtttccac catcagtggt
    39661 tgccggacga gctgcgtgtc gaaaaagggt ttagcccgga tacgatcaag ctgctggaag
    39721 caaaaggtca gaaagtggcg ctgaaagagg cgatgggcag tacacaaagc attatggttg
    39781 ggccggacgg tgagctgtac ggtgcatccg acccgcgctc ggtggatgat ttaacggcgg
    39841 ggtattaagg ttagcggccc tcttagtggg aagagggccg tattgtcagg gcaagccgaa
    39901 ggtagccttt tttatttcat actcctgtag atattcttcc agtggctttg ccgttcccag
    39961 agaatgaatt tcaccactgc ctttatcaac aataaaaggt gcgttaccag ctaagcgcgc
    40021 ggcctcatct ccagtttcga gaaattctcg tgcttcgaaa cagaaatacc agccctggct
    40081 aaagcgtcca tgtagagtaa tgacgaccgg gagatttgca tcatcaaggt aattgttcgc
    40141 tttcgcgaat gcgtcgtgat aagtaatcat aattataata aatattattg tttagcgtta
    40201 tattattatt cctgcgttat acattgagca aagacgcgtt catcttcgtc aaagatatta
    40261 ttaaatgctg gttttgaaaa gagttcatcg gataaggtat agtcacaaaa taagtatctg
    40321 ctaatatttt gtgatttatt ttccagaacg tcgaaccgga tttgctcatt taagatatca
    40381 gaatcttatg attcgggtaa aattctaacc aaatcaacat gtcactaaga tcatcatcgc
    40441 cattagctat ttcagtttaa atagcgacta catgtcttcg gtccatctca cttaaggagt
    40501 gtagttccgt tgtaagtttt tctatagctt gtattgataa atttcgatca agaaatttac
    40561 tgctggtaaa ctataaaaag atggcatggt ttacaatgat tttggttacc tttttattat
    40621 tatgaacaac tgtccatgat
//
LOCUS       DADSYM010000020        40310 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2415
            SAMN08039579-rid11723173.denovo.020, whole genome shotgun sequence.
ACCESSION   DADSYM010000020 DADSYM010000000
VERSION     DADSYM010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039579
            Sequence Read Archive: SRR6362533
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 40310)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40310)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980162.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 03:39:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,484
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 4,347
            CDSs (with protein)               :: 4,347
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 43 of 85
            Pseudo Genes (incomplete)         :: 37 of 85
            Pseudo Genes (internal stop)      :: 19 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40310
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039579-rid11723173.denovo.020"
                     /strain="LGE2415"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8302 N 95.9628 W"
                     /collection_date="2015-08-24"
     gene            <1..1057
                     /locus_tag="J1Y83_000912"
     CDS             <1..1057
                     /locus_tag="J1Y83_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020238717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA7629087.1"
                     /translation="KRKVLAMLVPALLVAGAANAAEIYNKNGNKVELYGKMVGERILT
                     DRENGEKGDNSQDTSYARVGVKGETQINPELTGYGQFELDLEASNRHNPDQTRLAYAG
                     LSYKDFGSFDYGRNVGVAYDAEAFTDMFVEWGGDSWAGTDLFMTNRTNGVATYRNTDF
                     FGMVEGLNFALQYQGKNEGTGNYKANGDGHGLSATYTIDGFSFAGAYANSDRTDWQSG
                     DGKGERAEVWALSTKYDANNVYAAVMYGESHNMNSDDGDVVNKTQNFEAVLQYQFDFG
                     LRPSIGYSYSKALDVAGYKDSDRLNYIEIGTWYYFNKNMNVYTAYQINLLDKSDYVLA
                     HGLNTDDQLAVGIVYQF"
     gene            1653..2504
                     /gene="hchA"
                     /locus_tag="J1Y83_000913"
     CDS             1653..2504
                     /gene="hchA"
                     /locus_tag="J1Y83_000913"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein deglycase HchA"
                     /protein_id="HBA7629088.1"
                     /translation="MTVQTSKNPQVDIAEDNAFFPSEYSLSQYTSPVSDLDGVDYPKP
                     YRGKHKILVIAADERYLPTDNGKLFSTGNHPIETLLPLYHLHAAGFEFEVATISGLMT
                     KFEYWAMPHKDEKVMPFFEQHKSLFRNPKKLADVVASLNADSEYAAIFVPGGHGALIG
                     LPESQDVAAALQWAIKNDRFVISLCHGPAAFLALRHSDNPLNGYSICAFPDAADKQTP
                     DIGYMPGHLTWYFGEELKKMGMNIINDDITGRVHKDRKLLTGDSPFAANALGKLAAQE
                     MLAAYAS"
     gene            complement(2612..3970)
                     /locus_tag="J1Y83_000914"
     CDS             complement(2612..3970)
                     /locus_tag="J1Y83_000914"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal sensor histidine kinase"
                     /protein_id="HBA7629089.1"
                     /translation="MKRLSITVRLTLLFILLLSVAGAGIVWTLYNGLASELKWRDDTT
                     LINRTAQIKQLLIDGVNPDTLPVYFNRMMDVSQDILIIHGDGINKIVNRTNVSDDMLN
                     NIPASETISAAGIYRSIINDTEIDAFRINIDEVSPSLTVTVAKLASARHNMLEQYKIN
                     SIIICIVAIILCSVLSPLLIRTGLREIKKLSGVTEALNYNDSRVPVEVSALPRELKPL
                     GQALNKMHHALVKDFERLSQFADDLAHELRTPINALLGQNQVTLSQTRSIAEYQKTIA
                     GNIEELENISRLTENILFLARADKNNVLVKLDALSLNKEVENLLDYLEYLSDEKEIRF
                     KVECNQQIFADKILLQRMLSNLIVNAIRYSPEKSRIHITSFLDANGSLNIDIASPGTK
                     INEPEKLFRRFWRGDNSRHSVGQGLGLSLVKAIAELHGGSATYHYLSKHNVFRITLPQ
                     RN"
     gene            complement(3970..4641)
                     /gene="hprR"
                     /locus_tag="J1Y83_000915"
     CDS             complement(3970..4641)
                     /gene="hprR"
                     /locus_tag="J1Y83_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor HprR"
                     /protein_id="HBA7629090.1"
                     /translation="MKILLIEDNQRTQEWVTQGLSEAGYVIDAVSDGRDGLYLALKDD
                     YALIILDIMLPGMDGWQILQTLRTAKQTPVICLTARDSVDDRVRGLDSGANDYLVKPF
                     SFSELLARVRAQLRQHHALNSTLEISGLKMDSVSQSVSRDNISITLTRKEFQLLWLLA
                     SRAGEIIPRTVIASEIWGINFDSDTNTVDVAIRRLRAKVDDPFPEKLIATIRGMGYSF
                     VAVKK"
     gene            4774..5187
                     /gene="hiuH"
                     /locus_tag="J1Y83_000916"
     CDS             4774..5187
                     /gene="hiuH"
                     /locus_tag="J1Y83_000916"
                     /EC_number="3.5.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyisourate hydrolase"
                     /protein_id="HBA7629091.1"
                     /translation="MLKRYLVLSVATAAFSLPSLVYAAQQNILSVHILNQQTGKPAAD
                     VTVTLEKKADNGWLQLNTAKTDKDGRIKALWPEQTATTGDYRVVFKTGDYFKKQNLES
                     FFPEIPVEFHINKVNEHYHVPLLLSQYGYSTYRGS"
     gene            5296..6300
                     /gene="msrP"
                     /locus_tag="J1Y83_000917"
     CDS             5296..6300
                     /gene="msrP"
                     /locus_tag="J1Y83_000917"
                     /EC_number="1.8.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase catalytic
                     subunit MsrP"
                     /protein_id="HBA7629092.1"
                     /translation="MKKNQFLKESDVTAESVFFMKRRQVLKALGISAAAFSLPQAAHA
                     DLLSWFKGNDRPPAPAGKPLEFSKPAAWQNNLPLTPADKVSGYNNFYEFGLDKADPAA
                     NAGSLKTDPWTLKISGEVAKPLTLDHDDLTRRFPLEERIYRMRCVEAWSMVVPWIGFP
                     LHKLLALAEPTSNAKYVAFETIYAPEQMPGQQDRFIGGGLKYPYVEGLRLDEAMHPLT
                     LMTVGVYGKALPPQNGAPVRLIVPWKYGFKGIKSIVSIKLTRERPPTTWNLAAPDEYG
                     FYANVNPHVDHPRWSQATERFIGSGGILDVQRQPTLLFNGYADQVASLYRGLDLRENF
                     "
     gene            6301..6936
                     /gene="msrQ"
                     /locus_tag="J1Y83_000918"
     CDS             6301..6936
                     /gene="msrQ"
                     /locus_tag="J1Y83_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase
                     heme-binding subunit MsrQ"
                     /protein_id="HBA7629093.1"
                     /translation="MRLTAKQVIWLKVCLHLAGLLPFLWLVWAINHGELGADPVKDIQ
                     HFTGRTALKFLLAALLITPLARYAKQPLLIRTRRLLGLWCFAWATLHLTSYALLELGV
                     NNLALLGKELITRPYLTLGIISWVILLALAFTSTQSMQRKLGKHWQQLHNFVYLVAIL
                     APIHYLWSVKIISPQPLIYAGLAVLLLALRYKKLLSLFNRLRKQAHNKLSL"
     gene            7194..7844
                     /gene="zinT"
                     /locus_tag="J1Y83_000919"
     CDS             7194..7844
                     /gene="zinT"
                     /locus_tag="J1Y83_000919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-binding protein ZinT"
                     /protein_id="HBA7629094.1"
                     /translation="MAIRLHKLAVALGVFIVSAPAFSHGHHSHGKPLTEVEQKAANGV
                     FDDTNVQNRTLSDWDGVWQSVYPLLQSGKLDPVFQKKADVDKTKTFAEIKDYYRKGYA
                     TDIEMIGIEDGIVEFHRNNETTSCKYDYDGYKILTYKSGKKGVRYLFECKDPESKAPK
                     YIQFSDHIIAPRKSSHFHIFMGNDSQQSLLNEMENWPTYYPYQLSSEEVVEEMMSH"
     gene            complement(8514..8603)
                     /locus_tag="J1Y83_000920"
     tRNA            complement(8514..8603)
                     /locus_tag="J1Y83_000920"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8567..8569),aa:Ser,seq:cga)
     gene            8697..9494
                     /gene="mtfA"
                     /locus_tag="J1Y83_000921"
     CDS             8697..9494
                     /gene="mtfA"
                     /locus_tag="J1Y83_000921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DgsA anti-repressor MtfA"
                     /protein_id="HBA7629095.1"
                     /translation="MIKWPWKVQESAHQTALPWQEALSIPLLTCLTEQEQSKLVALAE
                     RFLQQKRLVPLQGFELNSLRSCRIALLFCLPVLELGLEWLDGFHEVLIYPAPFVVDDE
                     WEDDIGLVHNQRIVQSGQSWQQGPIVLNWLDIQDSFDASGFNLIIHEVAHKLDTRNGD
                     RASGVPFISLREVAGWEHDLHAAMNNIQEEIELVGENAASIDAYAASDPAECFAVLSE
                     YFFSAPELFAPRFPSLWQRFCQFYQQDPLQRLHHANDTDSFSATNVH"
     gene            9595..9670
                     /locus_tag="J1Y83_000922"
     tRNA            9595..9670
                     /locus_tag="J1Y83_000922"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9628..9630,aa:Asn,seq:gtt)
     gene            9832..11094
                     /locus_tag="J1Y83_000923"
     CDS             9832..11094
                     /locus_tag="J1Y83_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HBA7629096.1"
                     /translation="MSLTDAKIRTLKPSDKPFKVSDSHGLYLLVKPGGSRHWYLKYRI
                     SGKESRIALGAYPAISQSDARQQREGIRKMLALNINPVQQRAAERGSRTPEKVFKNVA
                     LAWHKSNRKWSQNTADRLRASLNNHIFPVIGNLPVSELKPRHFIDLLKGIEEKGLLEV
                     ASRTRQHLSNIMRHAVHQELIDTNPAANLGGVTTPPVRRHYPALPLERLPELLERIGA
                     YHQGRELTRHAVLLMLHVFIRSSELRFARWSEIDFTNRVWTIPATREPIIGVRYSGRG
                     AKMRMPHIVPLSEQSIAILKQIKDITGNNELIFPGDHNPYKPMCENTVNKALRVMGYD
                     TKKDICGHGFRAMACSALMESGLWAKDAVERQMSHQERNTVRMAYIHKAEHLEARKAM
                     MQWWSDYLEACRESYAPPYTIGKNKFIP"
     gene            complement(11288..12592)
                     /gene="ybtS"
                     /locus_tag="J1Y83_000924"
     CDS             complement(11288..12592)
                     /gene="ybtS"
                     /locus_tag="J1Y83_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="yersiniabactin biosynthesis salicylate synthase
                     YbtS"
                     /protein_id="HBA7629097.1"
                     /translation="MKISEFLHLALPEEQWLPTISGVLRQFAEEECYVYERQPCWYLG
                     KGCQARLHINADGTQATFIDDAGEQKWAVDSIADCARRFMAHPQVKGRRVYGQVGFNF
                     AAHARGIAFNAGEWPLLTLTVPREELIFEKGNVTVYADSADGCRRLCEWVKEAGTTTQ
                     NAPLAVDTALNGETYKQQVARAVAEIRRGEYVKVIVSRAIPLPSRIDMPATLLYGRQA
                     NTPVRSFMFRQEGREALGFSPELVMSVTGNKVVTEPLAGTRDRMGNPEHNKAKEAELL
                     HDSKEVLEHILSVKEAIAELEAVCQPGSVVVEDLMSVRQRGSVQHLGSGVSGQLAENK
                     DAWDAFTVLFPSITASGIPKNAALNAIMQIEKTPRELYSGAILLLDDTRFDAALVLRS
                     VFQDSQRCWIQAGAGIIAQSTPERELTETREKLASIAPYLMV"
     gene            complement(12620..13900)
                     /gene="ybtX"
                     /locus_tag="J1Y83_000925"
     CDS             complement(12620..13900)
                     /gene="ybtX"
                     /locus_tag="J1Y83_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="yersiniabactin-associated zinc MFS transporter
                     YbtX"
                     /protein_id="HBA7629098.1"
                     /translation="MSDVQSNVKPLTLTTGRVIFAIAGVYVTQSLVSALSMQSLPALV
                     RAAGGSLALAGATTLFMLPWALKFIWAPWIERWRLPPGSQERRSRMLILRGQVALAAI
                     LTIAAAIGWFGREGGFPDTQIVALFVLFMVAGTVASTIDIASDGFCVDQLTRTGYGWG
                     NSVQVGGSYLGMMCGGGVFLMLSAASGWPVAMLMMAVLIMALSLPLWRITEPTRTATI
                     PHVPALGYALRRKQARLGLLLVLMLNSGMRFVLPLLAPLLLDHGLSMSALGALFSGGN
                     IAAGIAGTLAGGLLMKYTSPGRALLTAYGVQGIALLAVVMTLMMAPGHLLLQILQCLV
                     IVQSISLACALVCLYATLMSLSSPLQAGVDFTLFQCTDAAIAILAGVIGGVVAQHFGY
                     AACFLFAGAFTLLAAWVAYIRLHSARELMTSAID"
     gene            complement(13893..15695)
                     /gene="ybtQ"
                     /locus_tag="J1Y83_000926"
     CDS             complement(13893..15695)
                     /gene="ybtQ"
                     /locus_tag="J1Y83_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="yersiniabactin ABC transporter
                     ATP-binding/permease protein YbtQ"
                     /protein_id="HBA7629099.1"
                     /translation="MKDNNPADNLAWRVIWRQLISSVGSQARMLRRSMLALLLAAFMQ
                     GIAFACLYPIIDALLRGDAPQLLNWAMAFSVAAIVTLVLRWYGLGFEYRGHLAQATHE
                     LRLRLGEQLRRVPLEKLQRGRAGEMNALLLGSVDENLNYVIAIANILLLTIVTPLTAS
                     LATLWIDWRLGLVMLLIFPLLVPFYYWRRPAMRRQMQTLGEAHQRLSGDIVEFAQGMM
                     VLRTCGSDADKSRALLAHFNALENLQTRTHRQGAGATMLIASVVELGLQVVVLSGIVW
                     VVTGTLNLAFLIAAVAMIMRFAEPMAMFISYTSVVELIASALQRIEQFMAIAPLPVAE
                     QSEMPERYDIRFDNVSYRYEEGDGHALNHVSLTFPAASMSALVGASGAGKTTVTKLLM
                     RYADPQQGQISIGGVDIRRLTPEQLNSLISVVFQDVWLFDDTLLANIRIARPQATRQE
                     VEEAARAAQCLEFISRLPQGWLTPMGEMGGQLSGGERQRISIARALLKNAPVVILDEP
                     TAALDIESELAVQKAIDNLVHNRTVIIIAHRLSTIAGAGNILVMEEGQVVEQGTHAQL
                     LSHHGRYQALWQAQMAARVWRDDGGSASGEWVHE"
     gene            complement(15682..17394)
                     /gene="ybtP"
                     /locus_tag="J1Y83_000927"
     CDS             complement(15682..17394)
                     /gene="ybtP"
                     /locus_tag="J1Y83_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="yersiniabactin ABC transporter
                     ATP-binding/permease protein YbtP"
                     /protein_id="HBA7629100.1"
                     /translation="MALAGLAALTSLGALLFLAWSLRDIRATPDAIPAWPLGGVIGCV
                     VLTFVLRLQAFNTSHYAAFHLENILRSRLARKALQLPPGVLQQMGSGSVAKVMLDDVK
                     SLHIFVADSTPLYARAIIMPLATIVILFWLDWRLAIATLGVLAFGSVVLVLARQRSED
                     MAQRYHKAREQVSAAVIEFVQAMPVVRTFDSGSTSFLRYQRALEEWVDVLKTWYRKAG
                     FSARFSFSILNPLPTLFVLIWSGYGLLHYGSFDFIAWVAVLLLGSGMAEAVMPMMMLN
                     NLVAQTRLSIQRIYQVLAMPELSLPQSDQQPQEASITFEQVSFHYPQARTGAALQEVS
                     FHVPAGQIVALVGPSGAGKSTVARLLLRYADPDKGHIRIGGVDLRDMQTDTLMKQLSF
                     VFQDNFLFADTIANNIRLGAPDTPLEAVIAAARVAQAHDFISALPEGYNTRVGERGVF
                     LSGGQRQRITIARALLQDRPILVLDEATAFADPENEAALIKALAAAMRGRTVIMVAHR
                     LSMVTQADVILLFSDGQLREMGNHTQLLAQGGLYQRLWQHYQQAQHWVPGGTQEEVVE
                     NERQ"
     gene            17651..18610
                     /gene="ybtA"
                     /locus_tag="J1Y83_000928"
     CDS             17651..18610
                     /gene="ybtA"
                     /locus_tag="J1Y83_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="yersiniabactin transcriptional regulator YbtA"
                     /protein_id="HBA7629101.1"
                     /translation="MMESPQTQSEISIHQLVVGKPANDGNIPAQCELLRCSLQEGMDI
                     LLWRGHFARPETLQLHDDLGRINFSCILEGTSRFAIQGLRRHTDWELARNRHYITHTP
                     DCRGSASYCGRFESITLSFSPETLALWVPDISAVIKNKIDSHCCCQQQRCNAETHLTA
                     QALRHALMRMHGGCSHEQKPSTLWLQGQSLVMLSLVLDEHREDASCLSCHFNPMERQK
                     LLRAKDLLLADLTQAPGVAELARESGLSVLKIKRGFRVLFNNSVYGLFQAERMQEARR
                     RLANGNTSVMTVAADLGYANASHFSAAFQKQFGVTPSTFKRGM"
     gene            18801..24925
                     /gene="irp2"
                     /locus_tag="J1Y83_000929"
                     /pseudo
     CDS             18801..24925
                     /gene="irp2"
                     /locus_tag="J1Y83_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227766.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="yersiniabactin non-ribosomal peptide synthetase
                     HMWP2"
     gene            24998..34489
                     /gene="irp1"
                     /locus_tag="J1Y83_000930"
     CDS             24998..34489
                     /gene="irp1"
                     /locus_tag="J1Y83_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="yersiniabactin polyketide synthase HMWP1"
                     /protein_id="HBA7629102.1"
                     /translation="MDNLRFSSAPTADSIDASIVQHYPDCEPVAVIGYACHFPESPDG
                     ETFWQNLLEGRECSRRFTREELLAVGLDVAIIDDPHYVNIGTVLDNADCFDATLFGYS
                     RQEAESMDPQQRLFLQAVWHALEHAGYAPGAVPHKTGVFASSRMSTYPGREALNVTEV
                     AQVKGLQSLMGNDKDYIATRAAYKLNLHGPALSVQTACSSSLVAVHLACESLRAGESD
                     MAVAGGVALSFPQQAGYRYQPGMIFSPDGHCRPFDASAEGTWAGNGLGCVVLRRLRDA
                     LLSGDPIISVILSSAVNNDGNRKVGYTAPSVAGQQAVIEEALMLAAIDDRQVGYIETH
                     GTGTPLGDAIEIEALRNVYAPRPQDQRCALGSVKSNMGHLDTAAGIAGLLKTVLAVSR
                     GQIPPLLNFHTPNPALKLEESPFTIPVSAQAWQDEMRYAGVSSFGIGGTNCHMIVASL
                     PDALNARLPNTDSGRKSTALLLSAASDSALRRLATDYAGALRENADASSLAFTALHAR
                     RLDLPFRLAAPLNRETAEALSAWASEKSGALVYSGHGASGKQVWLFTGQGSHWRTMGQ
                     TMYQHSTAFADTLDRCFSACSEMLTPSLREAMFNPDSAQLDNMAWAQPAIVAFEIAMA
                     AHWRAEGLKPDFAIGHSVGEFAAAVVCGHYTIEQVMPLVCRRGALMQQCASGAMVAVF
                     ADEDTLMPLARQFELDLAANNGTQHTVFSGPEARLAVFCATLSQHDINYRRLSVTGAA
                     HSALLEPILDRFQDACAGLHAEPGQIPIISTLTADVIDESTLNQADYWRRHMRQPVRF
                     IQSIQVAHQLGARVFLEMGPDAQLVACGQREYRDNAYWIASARRNKEASDVLNQALLQ
                     LYAAGVALPWADLLAGDGQRIAAPCYPFDTERYWKERVSPACEPADAALSAGLEVASR
                     AATALDLPRLEALKQCATRLHAIYVDQLVQRCTGDAIENGVDAMTIMRRGRLLPRYQQ
                     LLQRLLNNCVVDGDYRCTDGRYVRARPIEHQQRESLLTELAGYCEGFQAIPDTIARAG
                     DRLYEMMSGAEEPVAIIFPQSASDGVEVLYQEFSFGRYFNQIAAGVLRGIVQTRQPRQ
                     PLRILEVGGGTGGTTAWLLPELNGVPALEYHFTDISALFTRRAQQKFADYDFVKYSEL
                     DLEKEAQSQGFQAQSYDLIVAANVIHATRHIGRTLDNLRPLLKPGGRLLMREITQPMR
                     LFDFVFGPLVLPLQDLDAREGELFLTTAQWQQQCRHAGFSKVAWLPQDGSPTAGMSEH
                     IILATLPGQAVSAVTFTAPSEPVLGQALTDNGDYLADWSDCAGQPEQFNARWQEAWRL
                     LSQRHGDALPVEPPPVAAPEWLGKVRLSWQNEAFSRGQMRVEARHPAGEWLPLSPAAP
                     LPAPQTHYQWRWTPLNVASIDHPLTFSFSAGTLARSDELAQYGIIHDPHASSRLMIVE
                     ESEDTLALAEKVIAALTASAAGLIVVTRRAWRVEENEALSASHHALWALLRVAANEQP
                     ERLLAAIDLAENTPWETLHQGLSAVSLSQRWLAARGDTLWLPSLAPNTGCAAELPANV
                     FTGDSRWHLVTGAFGGLGRLAVNWLREKGARRIALLAPRVDESWLRDVEGGQTRVCRC
                     DVGDAGQLATVLDDLAANGGIAGAIHAAGVLADAPLQELDDHQLAAVFAVKAQAASQL
                     LQTLRNHDGRYLILYSSAAATLGAPGQSAHALACGYLDGLAQQFSTLDAPKTLSVAWG
                     AWGESGRAATPEMLATLASRGMGALSDAEGCWHLEQAVMRGAPWRLAMRVFTDKMPPL
                     QQALFNISATEKAATPVIPPADDNAFNGSLSDETAVMAWLKKRIAVQLRLSDPASLHP
                     NQDLLQLGMDSLLFLELSSDIQHYLGVRINAERAWQDLSPHGLTQLICSKPEATPAAS
                     QPEVLRHDADERYAPFPLTPIQHAYWLGRTHLIGYGGVACHVLFEWDKRHDEFDLAIL
                     EKAWNQLIARHDMLRMVVDADGQQRILATTPEYHIPRDDLRALSPEEQRIALEKRRHE
                     LSYRVLPADQWPLFELVVSEIDDCHYRLHMNLDLLQFDVQSFKVMMDDLAQVWRGETL
                     APLAITFRDYVMAEQARRQTSAWHDAWDYWQEKLPQLPLAPELPVVETPPETPHFTTF
                     KSTIGKTEWQAVKQRLQQQGVTPSAALLTLFAATLERWSRTTTFTLNLTFFNRQPIHP
                     QINQLIGDFTSVTLVDFNFSAPVTLQEQMQQTQQRLWQNMAHSEMNGVEVIRELGRLR
                     GSQRQPLMPVVFTSMLGMTLEGMTIDQAMSHLFGEPCYVFTQTPQVWLDHQVMESDGE
                     LMFSWYCMDNVLEPGAAEAMFNDYCAILQAVIAAPESLKTLASGIAGHIPRRRWPLNA
                     QADYDLRDIEQATLEYPGIRQARAEITEQGALTLDIVMADDPSPSAAMPDEHELTQLA
                     LPLPEQAQLDELEATWRWLEARALQGIAATLNRHGLFTTPEIAHRFSAVVQALSAQAS
                     HQRLLRQWLQCLTEREWLIREGESWRCRIPLSEIPEPQEACPQSQWSQALAQYLETCI
                     ARHDALFSGQCSPLELLFNEQHRVTDALYRDNPASACLNRYTAQIAALCSAERILEVG
                     AGTAATTAPVLKATRNTRQSYHFTDVSAQFLNDARARFHDESQVSYALFDINQPLDFT
                     AHPEAGYDLIVAVNVLHDASHVVQTLRRLKLLLKAGGRLLIVEATERNSVFQLASVGF
                     IEGLSGYRDFRRRDEKPMLTRSAWQEVLVQAGFANELAWPAQESSPLRQHLLVARSPG
                     VNRPDKKAVSRYLQQRFGTGLPILQIRQREALFTPLHAPSDAPTEPAKPTPVAGGNPA
                     LEKQVAELWQSLLSRPVARHHDFFELGGDSLMATRMVAQLNRRGIARANLQDLFNHST
                     LSDFCAHLQAATSGEDNPIPLCQGDGEETLFVFHASDGDISAWLPLASALNRRVFGLQ
                     AKSPQRFATLDQMIDEYVGCIRRQQPHGPYVLAGWSYGAFLAAGAAQRLYAKGKQVRM
                     VLIDPVCRQDFCCANRAALLRLLAEGQTPLALPEHFDQQTPDSQLADFISLAKTAGMV
                     SQNLTLQAAETWLDNIAHLLRLLTEHTPGESVPVPCLMVYAAGRPAHWTPAETEWQGW
                     INNADDAVIEASHWQIMMEAPHVQACAQHITRWLCATSTQPENTL"
     gene            34486..35586
                     /gene="ybtU"
                     /locus_tag="J1Y83_000931"
     CDS             34486..35586
                     /gene="ybtU"
                     /locus_tag="J1Y83_000931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="yersiniabactin biosynthesis oxidoreductase YbtU"
                     /protein_id="HBA7629103.1"
                     /translation="MMPSASPKQRVLIVGAKFGEMYLNAFMQPPEGLELVGLLAQGSA
                     RSRELAHAFGIPLYTSPEQITRMPDIACIVVRSTVAGGTGTQLARHFLTRGVHVIQEH
                     PLHPDDISSLQTLAQEQGCCYWVNTFYPHTRAGRTWLRDAQQLRRCLAKTPQVVHATT
                     SRQLLYSTLDLLLLALGVDAAAVECDVVGSFSDFHCLRLFWPEGEACLLLQRYLDPDD
                     PDMHSLIMHRLLLGWPEGHLSLEASYGPVIWSSSLFVADHQENAHSLYRRPEILRDLP
                     GLTRSAAPLSWRDCCETVGPEGVSWLLHQLRSHLAGEHPPAACQSVHQIALSRLWQQI
                     LRKTGNAEIRRLTPPHHDRLAGFYNDDDKEAL"
     gene            35649..36386
                     /gene="ybtT"
                     /locus_tag="J1Y83_000932"
     CDS             35649..36386
                     /gene="ybtT"
                     /locus_tag="J1Y83_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="yersiniabactin biosynthesis thioesterase YbtT"
                     /protein_id="HBA7629104.1"
                     /translation="MCPFAGGSSSAFRHWQAEQLADCALSLVTWPGRDRLRHLEPLRS
                     ITQLAALLANELEASVSPDTPLLLAGHSMGAQVAFETCRLLEQRGLAPQGLIISGCHA
                     PHLHSERQLSHRDDADFIAELIDIGGCSPELRENQELMSLFLPLLRADFYATESYHYD
                     SPDVCPPLRTPALLLCGSHDREASWQQVDAWRQWLSHVTGPVVIDGDHFYPIQQARSF
                     FTQIVRHFPHAFSAMTAWQKQPSTSER"
     gene            36390..37967
                     /gene="ybtE"
                     /locus_tag="J1Y83_000933"
     CDS             36390..37967
                     /gene="ybtE"
                     /locus_tag="J1Y83_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="yersiniabactin biosynthesis salycil-AMP ligase
                     YbtE"
                     /protein_id="HBA7629105.1"
                     /translation="MNSSFESLIEQYPLPIAEQLRHWAARYASRIAVVDAKGSLTYSA
                     LDAQVDELAAGLSSLGLRSGEHVIVQLPNDNAFVTLLFALLRLGVIPVLAMPSQRALD
                     IDALIELAQPVAYVIHGENHAELARQMAHKHACLRHVLVAGETMSDDFTPLFSLHGER
                     QAWPQPDVSTTALLLLSGGTTGTPKLIPRRHADYSYNFSASAELCGISQQSVYLAVLP
                     VAHNFPLACPGILGTLACGGKVVLTDSASCDEVMPLIAQERVTHVALVPALAQLWVQA
                     REWEDSDLSSLRVIQAGGARLDPTLAEQVIATFDCTLQQVFGMAEGLLCFTRLDDPHA
                     TILHSQGRPLSPLDEIRIVDQDENDVAPGETGQLLTRGPYTISGYYRAPAHNAQAFTA
                     QGFYRTGDNVRLDEVGNLHVEGRIKEQINRAGEKIAAAEVESALLRLAEVQDCAVVAA
                     PDTLLGERICAFIIAQQVPTDYQQLRQQLTRMGLSAWKIPDQIEFLDHWPLTAVGKID
                     KKRLTALAVDRYRHSAQ"
     gene            38098..40119
                     /gene="fyuA"
                     /locus_tag="J1Y83_000934"
     CDS             38098..40119
                     /gene="fyuA"
                     /locus_tag="J1Y83_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore yersiniabactin receptor FyuA"
                     /protein_id="HBA7629106.1"
                     /translation="MKMTRLYPLALGGLLLPAIANAQTSQQDESTLVVTASKQSSRSA
                     SANNVSSTVVSAPELSDAGVTASDKLPRVLPGLNIENSGNMLFSTISLRGVSSAQDFY
                     NPAVTLYVDGVPQLSTNTIQALTDVQSVELLRGPQGTLYGKSAQGGIINIVTQQPDST
                     PRGYIEGGVSSRDSYRSKFNLSGPIQDGLLYGSVTLLRQVDDGDMINPATGSDDLGGT
                     RASIGNVKLRLAPDDQPWEMGFAASRECTRATQDAYVGWNDIKGRKLSISDGSPDPYM
                     RRCTDSQTLSGKYTTDDWVFNLISAWQQQHYSRTFPSGSLIVNMPQRWNQDVQELRAA
                     TLGDARTVDMVFGLYRQNTREKLNSAYDMPTMPYLSSTGYTTAETLAAYSDLTWHLTD
                     RFDIGGGVRFSHDKSSTQYHGSMLGNPFGDQGKSNDDQVLGQLSAGYMLTDDWRVYTR
                     VAQGYKPSGYNIVPTAGLDAKPFVAEKSINYELGTRYETADVTLQAATFYTHTKDMQL
                     YSGPVGMQTLSNAGKADATGVELEAKWRFAPGWSWDINGNVIRSEFTNDSELYHGNRV
                     PFVPRYGAGSSVNGVIDTRYGALMPRLAVNLVGPHYFDGDNQLRQGTYATLDSSLGWQ
                     ATERMNISVYVDNLFDRRYRTYGYMNGSSAVAQVNMGRTVGINTRIDFF"
BASE COUNT         9234 a        11520 c        10603 g         8953 t
ORIGIN      
        1 gaaaagaaaa gttctggcaa tgctggtccc ggcgttatta gttgctggcg cagcaaatgc
       61 ggctgaaatt tataataaaa atggcaataa agttgagcta tacggcaaga tggttggtga
      121 gcgcattctg accgaccgtg aaaatggtga aaaaggcgat aattctcaag atacctctta
      181 tgcgcgtgta ggcgtgaaag gtgaaactca gatcaaccct gaactgactg gttacggtca
      241 gtttgaacta gacctggaag catcaaacag acacaaccct gaccaaactc gtttggcata
      301 tgctggtttg agttacaaag attttggttc attcgactac ggtcgtaatg tcggtgtcgc
      361 gtatgatgca gaagcattta ctgatatgtt tgttgagtgg ggcggtgact catgggctgg
      421 tactgacctg ttcatgacca accgtaccaa cggtgttgca acctatcgta acacagattt
      481 ctttggcatg gttgaaggtc taaattttgc tctgcaatat cagggtaaaa atgaaggtac
      541 aggcaactac aaagcaaatg gcgatggcca tggtttatct gcaacttaca ctattgacgg
      601 tttctcattt gctggtgctt atgcaaactc tgaccgtact gattggcagt ctggtgatgg
      661 taaaggcgaa agagccgaag tttgggcgtt gtcaacaaaa tatgacgcta acaatgtcta
      721 tgctgctgtg atgtacggtg aatctcacaa catgaattcc gacgacggtg atgttgtgaa
      781 caaaacacaa aacttcgaag ctgttctgca atatcagttc gactttggtc tgcgtccatc
      841 catcggttac tcctattcta aagcactgga cgtagcaggt tacaaagatt ctgatcgtct
      901 gaactatatt gaaatcggta cctggtatta cttcaataag aatatgaacg tatataccgc
      961 ttatcagatc aacctgcttg ataagagtga ttatgttctc gctcatggac tgaacaccga
     1021 cgaccaactg gcagttggta tcgtctacca gttctaatca gccaacgcgc cgaggcgtgt
     1081 tactgtaaaa cataacacca gcctgagttc gtccctcatc gaactcaggc tttttttatt
     1141 ggcatttcat ctccatctgc ggcacacgca attgccaaag catctcgata cagcccctta
     1201 ccctgcccct cctggcacaa tatcctctac cagcaactct tccagccaac accccaaatg
     1261 catttcttta taaatttaat ctgtaacaaa accgcttccc atatagaaat atttgcaaaa
     1321 atagtgcatg aaaaaagctt tttccctacc aatccgggat ataaatattc atcagtacca
     1381 caacattcca cagcactaaa tctctcccca tcgcccccaa acctctgata atggtctaaa
     1441 atcattgagg ccacttgcga cgacgttact caataaggaa agtttggcgt gaataatatt
     1501 caacaccagt gcagcatcat cattacatac ttatgcccgc caactaccgc tttcgatata
     1561 ttgactcaat ttttcagcct ggttaaaaat agcgaccagc tcagtcgcaa atatagtgac
     1621 taccctaact aagcaacaat aaggaataca ctatgactgt tcaaacaagt aaaaacccgc
     1681 aggtcgatat tgctgaagat aatgcgttct tcccttcaga atattcgctt agccaatata
     1741 cgagtcccgt ctctgatctt gatggcgtgg actatccaaa accgtatcgc ggtaaacata
     1801 aaattctggt gatcgccgcg gacgaacgtt atttgccgac cgataacgga aaactgttct
     1861 cgaccggtaa ccatccgatt gaaacgttgc tgccgttgta tcatctccat gctgcaggtt
     1921 tcgaattcga agtggcgacc atttccggtc tgatgaccaa gtttgaatac tgggctatgc
     1981 cgcacaaaga tgaaaaagta atgccattct ttgaacagca taaatcgtta ttccgtaatc
     2041 cgaagaaact cgcggatgtt gtcgccagcc tcaacgctga tagcgaatat gcagcaatct
     2101 ttgttcctgg tgggcatggc gcacttattg gtttaccaga aagccaggac gtggctgccg
     2161 ctttacagtg ggcaatcaaa aatgaccgtt ttgttatctc cctttgccac ggtccggcgg
     2221 cttttctggc acttcgccac agcgataacc cactgaatgg ttattctatt tgcgcattcc
     2281 cagacgccgc agacaaacaa acgccagata ttggctatat gccgggtcat ctcacctggt
     2341 acttcggcga agagttgaag aaaatgggca tgaacatcat taatgacgac atcaccgggc
     2401 gagtacataa ggaccgtaaa cttctcaccg gcgacagtcc ttttgcagcg aatgcgttgg
     2461 gtaaactggc ggcgcaggaa atgctggcag cttacgcgag ttaatccctg ttacattgta
     2521 atgtaagcgc ataatcaatc gcagccacgt taatactcac gacgtggctg cgttttttca
     2581 cggttaactt atggcgtact gaagcccgat gttaatttct ttgcggtaac gtaattcgga
     2641 acacgttatg cttactgaga tagtgatacg tagcacttcc tccatgtaat tcggcaatcg
     2701 ctttgactaa agaaaggcca agtccctgac ctacggaatg acgcgaatta tctccccgcc
     2761 aaaatctacg gaagagtttt tcaggctcat taattttcgt tccaggactg gcgatatcaa
     2821 tattaagaga accgttggca tcaagaaaac tggttatatg aatacgcgat ttttctggtg
     2881 aatatcgaat ggcgttaaca ataagattcg ataacattcg ttgcagtaaa attttatccg
     2941 caaagatttg ttgattgcac tcgaccttaa aacgaatctc tttctcgtct gaaagatatt
     3001 caagatagtc caaaagattt tcaacttcct tattgagaga aagcgcatcc agtttcacca
     3061 aaacattgtt tttgtctgcc ctggcaagaa acagtatatt ctctgttaac cgcgaaatat
     3121 tttccagctc ttcaatgttt ccggcaattg ttttttgata ttcagcgata cttctggttt
     3181 gactgagcgt aacctgattc tgacccagta atgcattaat tggcgttcta agttcatgag
     3241 cgagatcgtc agcaaattga ctaaggcgct caaaatcttt gactaaagca tgatgcattt
     3301 tattcaacgc ctgcccaaga ggttttagtt ctctcggtaa tgcgctaacc tcaaccggca
     3361 ctcggctatc gttataattc agcgcttccg ttacgccact caactttttg atctctcgca
     3421 accccgttct gattaacaac ggacttaata ctgagcacaa tataatggcg acaatgcaaa
     3481 ttataatgct attaatttta tactgttcaa gcatgttatg tctggctgaa gccaatttag
     3541 ccacagtaac cgttaatgat ggcgaaactt catcaatatt aattcggaaa gcatctatct
     3601 ctgtatcatt aataatgctt ctgtaaatgc ctgctgcgct gattgtctca ctagcaggta
     3661 tgttatttaa catgtcatca ctgacatttg tccggttaac aattttattg atgccatcac
     3721 catgaatgat caaaatatcc tgactaacat ccatcatccg gttaaagtac acaggtaacg
     3781 tatctggatt taccccatca attaacaact gtttgatctg cgctgtccgg ttaatgagtg
     3841 ttgtatcatc gcgccatttc aactcacttg ccaggccatt atagagagtc catacaattc
     3901 cagcgccagc aacagacagc agcaatataa aaagcaaggt taaacggacg gttatagata
     3961 atcttttcat tattttttta ccgctacgaa tgaatagccc atcccccgga ttgtggcgat
     4021 tagcttttca ggaaaaggat catcaacttt tgcgcggagc ctgcgaatgg cgacatccac
     4081 cgtattggta tcactatcaa agttgattcc ccaaatttca ctcgcaataa ccgttctggg
     4141 tataatctcg ccagctctgg aggccagtag ccaaagtaac tgaaactcct tgcgcgtcag
     4201 tgtaatactg atattgtccc tgctcacact ttgactgaca gagtccattt ttaagccact
     4261 gatttctaat gttgaattca gagcgtgatg ttgccttaat tgtgcccgaa cccttgccag
     4321 caactcagaa aatgaaaaag gttttaccag ataatcattt gccccactgt ccagccctct
     4381 gactctgtca tcgacagaat cccttgcagt aaggcaaata acaggggttt gctttgccgt
     4441 tcttaacgtt tgtaagatct gccagccatc catacccgga agcataatat ccagaatgat
     4501 caatgcataa tcatccttca gcgcaagata aagcccatct ctgccatcag aaacggcatc
     4561 aatgacataa cccgcttcgg aaagcccctg cgttacccat tcctgggtcc tttgattatc
     4621 ttcaataagt agaatcttca tgaaaataat atgccataaa tcatatgtta tagaaacaac
     4681 ctggttcatt acaaaattgt aatgctgctg taaggttacc ctggacgttt tttcgctatc
     4741 ctcaaaactc attcacatga caaggatata aacatgttaa agcgttattt agtactctcg
     4801 gtagcaacgg cagcattttc attaccttct ttggtttatg ccgcacaaca aaacattctt
     4861 agcgtgcaca ttttgaacca gcaaacagga aaacccgctg ccgacgtgac agtcactctt
     4921 gaaaagaaag cggataacgg ctggttacaa cttaataccg ccaaaacaga taaggatgga
     4981 cgaattaagg cactgtggcc cgagcaaact gcgactactg gcgattaccg tgtcgtattt
     5041 aaaaccgggg actatttcaa gaaacaaaat cttgaaagtt tcttccctga gatccccgtt
     5101 gaatttcata ttaataaagt gaacgagcat tatcatgtgc ctttgttact cagccaatat
     5161 gggtattcaa cctatcgtgg cagttaatgc gtaaatatgt aaaagctgat ctctgctact
     5221 gttagggagg tgactccctg acagattaac agtaaacggc tcttgttggc taacgacaaa
     5281 aaagtgtgat ggcttatgaa aaagaatcaa tttttaaaag aatcagatgt tacggccgag
     5341 tcggtattct ttatgaagcg tcggcaggtg ttaaaagcac tgggcatcag cgcagctgca
     5401 ttttctttgc ctcaagctgc gcatgccgat ctgcttagct ggtttaaagg gaacgatcgt
     5461 ccgcccgccc ccgccggaaa accgctggag ttcagcaagc ctgccgcctg gcaaaataac
     5521 ctgccactga cgccagcaga taaagtctcc ggttataaca acttctatga attcgggctg
     5581 gataaagccg atcccgccgc taatgctggt agcctgaaaa ccgatccatg gacattgaaa
     5641 atcagcggcg aagtggcaaa accattgacc ctcgatcacg atgatttaac ccgtcgcttc
     5701 ccgctggaag agcgtattta tcgtatgcgc tgcgtagaag cgtggtcgat ggtggtgccg
     5761 tggattggtt ttccgctaca caaattgctg gcgcttgccg aacccaccag caatgcgaag
     5821 tatgtcgctt tcgaaacgat ttatgcgccg gaacagatgc ctggccagca ggaccgcttt
     5881 atcggcggcg ggctgaaata tccttatgtc gaaggattgc gtctcgacga agcaatgcat
     5941 ccgctcacac tgatgaccgt aggtgtttat ggcaaggcgt taccgccaca aaatggcgcg
     6001 ccggtgcgac tgattgtgcc gtggaaatat ggctttaaag ggattaaatc gatcgtcagt
     6061 attaagctga cccgcgagcg tccgccaacc acctggaatc tggcagcgcc tgacgaatac
     6121 ggtttttacg ccaacgttaa tccgcatgtt gatcacccgc gctggtcaca ggctaccgaa
     6181 cgatttattg gttcaggcgg cattctcgat gttcagcgcc agccaacact actgtttaat
     6241 ggttacgccg accaggtggc atcgctgtat cgtggcctgg atttgcggga gaatttctaa
     6301 atgcgtctga cagcaaaaca ggtgatatgg ctgaaagttt gcctgcatct tgccggattg
     6361 ttgccgtttc tctggctggt ctgggcgatc aatcacggtg aactgggtgc cgatccggtg
     6421 aaagatattc agcattttac cggtcgcact gcactgaaat ttttgcttgc ggccttgtta
     6481 atcacacctc tggcacgcta cgcaaaacag ccgttattga tacgcactcg ccgcctgtta
     6541 ggcttatggt gctttgcctg ggcgacactg catttaacca gttacgcatt gctggagttg
     6601 ggcgtgaata atctggcgtt attaggtaag gagttaatta cccggcctta tttaacgtta
     6661 ggaattatca gctgggttat tttgcttgct ttagcgttca cttcaaccca gtcgatgcag
     6721 cgaaaactcg gcaaacactg gcaacagttg cataacttcg tctatcttgt cgcgatcctg
     6781 gccccaatac attatctgtg gtcagtgaag attatttcac cgcagccact catctacgct
     6841 gggctggctg tactgctttt agccttacgg tataagaagt tgctttccct tttcaaccgg
     6901 ttacgcaagc aggcccacaa caaattatct ctataacttc atattgccga caaacttcgc
     6961 gttatcccgg ccggatgcgg cgcgagcgcc ctatccggcc cacacagatc gcatcaagtt
     7021 atgtttcgtt tgtcatcaac ctttcataac cagaataatt tattttattt caataagata
     7081 gttcaataca cctgagaata ccatgctctc atttcctaat gagttgttgc attttttcat
     7141 atgttacaat ataacattac acatcatgta cattaactct ggaggaaatt gttttggcga
     7201 ttcgtcttca taaactggct gttgctttag gtgtctttat tgttagcgct cctgcctttt
     7261 cgcatggtca tcattctcat ggcaaaccct taacagaggt cgaacaaaaa gctgctaatg
     7321 gtgtttttga tgataccaat gtacaaaacc gaacgctcag tgactgggat ggagtctggc
     7381 aatccgttta ccctttactg caaagtggca aacttgatcc cgtctttcag aagaaagcgg
     7441 atgtagataa aaccaaaaca tttgctgaaa ttaaagatta ttatcgcaaa ggttatgcga
     7501 cagatatcga aatgattggc attgaggacg gcattgtcga attccataga aataatgaaa
     7561 caacatcctg taaatatgat tacgatggat acaaaatact cacctataaa tcaggcaaga
     7621 aaggcgttcg ttacttattc gaatgtaaag atcctgaaag caaagcccct aaatatatac
     7681 aatttagcga tcatataatt gcaccacgaa aatcatctca ttttcacatt tttatgggta
     7741 atgattccca acagtcttta ttgaatgaaa tggaaaactg gccaacgtat tatccctatc
     7801 agttgagtag cgaagaagtg gtcgaggaga tgatgtctca ttgatttttt tgcgctgact
     7861 tcagttattt aacagaacag tgcctggtag taacaacaat aatttaccaa gacaaataag
     7921 taagacaaac tcagacacgc tcatacttga gtcgtgtctt ttttattatg tctgtagaaa
     7981 aaaactgaga aaaaggcgaa caataaatac acaactcagc catacagact agcagcgcaa
     8041 atgtttccag gagcaagtca tctgtcatgg taaagctaac cagaaatata ggttaatttt
     8101 ggtgtttaaa ccttttttac ttgttagtat gatacaaagg ctttcaaaaa agctgggaaa
     8161 ttcaacaact cttggaaatt atggactttg tcccgcggct ttaatctgac ggccgcgttc
     8221 cttttttggc tcaattattg accccttctc tacgcaactt cagttcccgc cagcaacttt
     8281 gcggcagttt tataggatca atgtctaaaa gaataatggt gaccgataag aaaacgactg
     8341 aataactgta gattttcgct cgaaaccttc ctgtcagctc catagcgaat caagtgctga
     8401 atgtcacagt atcgaacaga aaacagtgac gatctaaccc ttcaagaata ttctacgatt
     8461 gttctgttta ggaaaagcaa ggcgggaagt cgggagataa gtcattgata aagtggcgga
     8521 gagaggggga tttgaacccc cggtagagtt gcccctactc cggttttcga gaccggtccg
     8581 ttcagccgct ccggcatctc tccgttcaga tggttgccat gatgccagga aatttggcat
     8641 tttaacagtc cctgtccgtg caattttgtt caagtgacga gtttgcgagc aaaacgatga
     8701 ttaagtggcc ctggaaagta caagaatcag cacatcaaac tgcccttccc tggcaggaag
     8761 cactatcgat ccccctttta acgtgtctga cagaacagga acaaagcaaa ttggtcgctc
     8821 ttgccgaacg ttttttacag caaaaacggc ttgttccttt acagggcttt gagctgaatt
     8881 cattaaggag ctgccggata gcacttctat tttgcctgcc cgttctggag ttaggactgg
     8941 aatggctgga tggttttcat gaagtcttaa tttatcctgc gccatttgtg gtcgatgatg
     9001 aatgggaaga cgatatcggt ctggtgcata accaacgtat tgttcagtca ggtcagagct
     9061 ggcagcaagg gcctatcgtt ttgaactggt tggatataca agattctttt gatgcttctg
     9121 gttttaacct gattattcat gaagtcgctc ataagctgga cacccgtaac ggcgatcgcg
     9181 ccagcggagt tccctttatt tcgttgcgtg aggttgctgg ctgggaacac gatcttcatg
     9241 ctgcaatgaa caacattcag gaagaaatcg aattagttgg tgagaatgcg gcgagcattg
     9301 atgcttatgc tgccagtgat cctgctgaat gttttgccgt actttctgaa tatttcttta
     9361 gcgccccaga actttttgct cctcgtttcc cttcattgtg gcaacgtttc tgtcaatttt
     9421 atcaacaaga tcctttgcag agactgcatc acgctaatga tacagactcg ttttcggcga
     9481 cgaatgttca ttaattaaca actttgcaga ttaattaacc aattgaaatg gcttatgaaa
     9541 tttagtgttg acagacaagg taccgctaag taatatgcgc cccgttctca cgattcctct
     9601 gtagttcagt cggtagaacg gcggactgtt aatccgtatg tcactggttc gagtccagtc
     9661 agaggagcca attttctgtt ttcttgcatc cttgcgaatc tttatctgat gttgattcaa
     9721 caggttagtg tgaaaactct tctcggtgca ttttgatttt accctacgca tcgggaaaaa
     9781 ttggtggtca aatctggggt caggttagtt cgataatgga gtgaccccca tatatccctt
     9841 accgacgcaa aaatccgcac cctcaagcct tctgataaac cctttaaagt ctccgattct
     9901 cacggtctgt atctgctggt caagccgggt ggctcccgcc actggtatct caaataccgt
     9961 attagcggta aagaatcccg cattgcgctg ggtgcctatc cagccatctc ccagtctgat
    10021 gcgcgacagc aacgtgaagg tatccgtaaa atgctggcgc tgaatatcaa cccggtacag
    10081 cagcgggctg ctgaacgtgg ctcacgaaca ccggagaaag tttttaaaaa cgtggcgctg
    10141 gcgtggcata aaagtaacag gaaatggtcg cagaacaccg ccgaccgtct gcgtgccagt
    10201 ctgaacaatc acatctttcc ggtcatcggg aacttacctg tatcagaact taaaccccgt
    10261 catttcattg acctgctgaa agggatcgag gaaaaaggtc tgctggaggt tgcgtcccgc
    10321 acacggcagc acctgagtaa cataatgcgc catgcggtcc atcaggagtt aatcgatacg
    10381 aaccctgcag caaaccttgg cggcgtgacc acacctcctg tcagacggca ctatcctgcc
    10441 ctgccgctgg agcggctgcc tgaactgctt gaacgtattg gggcatatca tcagggccgt
    10501 gaactgaccc ggcatgccgt tctgctgatg ctgcatgtgt tcattcgctc cagtgaactg
    10561 cgtttcgccc gctggtcaga gattgatttc acaaaccgag tctggacgat acccgcgacg
    10621 cgagaaccca ttattggcgt gcgttattcc ggccgcgggg caaaaatgcg aatgccgcat
    10681 atcgtccccc tctcagaaca gtccatcgcc attctgaaac agattaagga tatcaccggt
    10741 aataatgaac tgatcttccc cggcgaccat aacccgtata agccaatgtg tgaaaacacg
    10801 gtcaataagg cactgcgggt gatgggttac gacacgaaaa aggatatctg cggtcacggc
    10861 ttccgggcaa tggcatgcag tgcgctgatg gaatcgggtt tatgggcaaa ggacgcagta
    10921 gaacgccaga tgagtcatca ggagcgcaat accgtgcgca tggcttatat tcataaggca
    10981 gagcacctgg aagcccgcaa agcgatgatg cagtggtggt cggattatct ggaagcatgc
    11041 cgagaatctt atgcaccgcc ttatacaatt ggtaaaaata agtttatccc atagtatact
    11101 aacgaatccc acactctggc aaattaccac cacctccaca ggaggtggtt ctatatagac
    11161 ttttatcaac ttcatgacga aaaacaccta ttttatccca aaagaagaca atagagtcac
    11221 agaggaatca aagagcgact caattgtctt tggacaatct ggttgtgagg ttagaaaaca
    11281 gttactccta caccattaaa tagggcgcaa tgctcgctaa tttctcccgg gtttctgtca
    11341 gttcgcgttc cggcgtagat tgcgcgatga ttcccgcccc cgcctgtatc cagcagcgct
    11401 ggctatcctg aaatacggaa cgcagaacta gcgccgcatc aaagcgcgta tcgtccagca
    11461 gcaggattgc gccggaataa agctctcgcg gcgtcttctc aatttgcata atcgcgttca
    11521 gagcagcatt tttagggata cctgaggcgg taatcgacgg aaacagcacg gtaaacgcat
    11581 cccaggcatc cttgttttcc gcaagctgac cgctcacgcc agaccccaga tgctgaacgc
    11641 tgccgcgctg gcgaaccgac attaaatctt caaccaccac gctgcccggc tggcaaacgg
    11701 cctccagttc agcaatagct tctttgacag aaaggatatg ctcaagcacc tctttactgt
    11761 cgtgcagcag ttctgcctct ttcgccttat tatgctccgg gtttcccatg cgatcgcggg
    11821 tgcccgcaag cggttcagtg accactttat tgcccgtcac tgacatcacc agttccgggc
    11881 taaagcccag cgcttcgcgt ccttcctgac ggaacataaa cgagcgcaca ggcgtgtttg
    11941 cctgccgccc gtataacagc gtggcgggca tatcaatccg cgatggcagg ggaatggcgc
    12001 gcgagacaat cactttgaca tactcgccac ggcggatctc cgcaacggcg cgcgcaacct
    12061 gttgtttata tgtctcacca ttgagggcgg tatccaccgc cagtggtgcg ttctgcgtcg
    12121 ttgtaccggc ctcttttacc cactcgcaca aacgtcggca cccgtcggcg gagtccgcat
    12181 aaacggtgac atttcccttt tcaaaaataa gttcttcacg gggaacggtt aacgtcagca
    12241 gcggccactc cccggcgtta aaggcaatcc cccgcgcatg cgccgcaaag ttgaacccaa
    12301 cctgtccata tacccgacgt cctttcacct gaggatgcgc cataaaacga cgcgcgcagt
    12361 cggcaatgga atccaccgcc catttttgct ccccggcatc atcaataaat gtcgcctgcg
    12421 ttccgtcggc attaatgtgc agccgtgcct ggcacccttt gcctaaatac caacagggtt
    12481 gacgctcata gacataacac tcttcttctg cgaactggcg taaaacgcca gaaatcgtcg
    12541 gtagccattg ttcctctggt aacgccagat gtagaaattc actgattttc atcccttacc
    12601 tctctgtgtt attcgcggat caatcaattg cgcttgtcat cagttctctt gccgaatgca
    12661 gccggatata agcaacccac gccgccagca acgtgaatgc cccggcaaac aggaagcagg
    12721 ccgcatagcc aaaatgttga gcaacaacgc caccgataac gccagccaga atggcgattg
    12781 ccgcgtcagt acattgaaag agggtgaagt cgacaccggc ctgcaaaggc gatgaaagcg
    12841 acatcagcgt ggcgtaaaga cagaccagcg cgcaggccag cgaaatggac tggacaatga
    12901 ccagacactg gagaatctgc agcagcagat gacccggcgc catcatgagc gtcatcacca
    12961 ccgccagcag cgcgatcccc tggacgccat aagccgtcaa cagcgctctg ccgggtgagg
    13021 tgtatttcat cagcaatccg ccggccagcg ttcctgctat gcccgctgca atattgccgc
    13081 cgctgaacag cgcgcccaat gcggacatgc tcaacccatg atccaacaac agcggtgcca
    13141 gaagaggcag cacaaaccgc atgcctgaat tcagcatcaa taccagcagt aagcccaggc
    13201 gcgcctgctt cctccttagc gcataaccta acgccggaac atgcgggata gtcgctgttc
    13261 gcgtcggctc cgtaatgcgc cacagcggga gtgacagcgc cataatcagc accgccatca
    13321 tcagcatggc gacaggccag ccggatgccg ccgacagcat caggaacacc ccgccgccgc
    13381 acatcattcc cagatagctg ccgccgacct gcacgctgtt tccccagccg taacccgtgc
    13441 gggtcagttg atcgacacaa aagccgtcgc tggcgatatc aatggtggag gcgaccgtgc
    13501 ctgccaccat aaacagaaca aataacgcga cgatttgcgt atcggggaat cccccttctc
    13561 gcccaaacca gccaatcgct gcggcaatag tcaggatcgc cgctagcgcg acctgaccac
    13621 gcaggattaa catgcgggaa cggcgttcct ggctaccggg cggaagtcgc cagcgctcga
    13681 tccacggcgc ccaaataaac ttcagcgccc agggcagcat aaacagggtt gtcgcaccgg
    13741 caagcgccag cgaaccgcca gcagcgcgca ccagcgcggg taaggactgc atagacagcg
    13801 ccgataccag actctgcgtc acatagacgc cggcaatagc aaaaatcacc cgcccggtcg
    13861 tcaacgtcag aggtttcaca ttcgactgaa catcactcat gcacccactc tccagacgcg
    13921 gaacccccgt cgtcgcgcca cacgcgcgcg gccatttgcg cctgccacaa cgcctgataa
    13981 cgtccatgat gtgagagcaa ttgcgcatga gtaccctgct caaccacctg tccctcttcc
    14041 atcaccagaa tgtttccggc cccggcgatg gtggataaac ggtgagcgat gataatcacc
    14101 gtccggttgt gaaccaggtt atcgatcgct ttttgcaccg ccagctcgct ttcaatatcc
    14161 agcgcggcag tcggttcatc gagaatgacg accggcgcgt ttttcaataa cgctctggca
    14221 atggaaatcc gctggcgctc gccgcccgat agctggccgc ccatctctcc cattggcgtc
    14281 agccagcctt gcggaagacg ggaaataaac tcaaggcact gcgccgcgcg ggcggcctct
    14341 tctacctcct gccgcgtcgc ctgtgggcgc gcgatacgga tattcgccag cagcgtgtca
    14401 tcaaacagcc agacatcctg gaaaacaaca gaaatcaggc tattgagctg ttccggcgtc
    14461 aggcggcgaa tatcgacgcc gccaatagaa atctgccctt gctgcggatc ggcatagcgc
    14521 attaacagtt tggtgacggt agttttgcct gcgccggagg cacccaccag cgcgctcata
    14581 ctggctgccg ggaacgtcaa agaaacatga ttaagcgcgt ggccgtcgcc ttcttcgtag
    14641 cgatagctga cgttgtcaaa gcggatatcg taacgttccg gcatctcgct ttgctctgcg
    14701 acgggaagcg gtgctatcgc cataaactgc tcaatccgtt gcagggcgct ggcgatcagt
    14761 tccacaaccg aggtgtagct gataaacatc gccatcggtt ctgcgaagcg cataatcatc
    14821 gcgacggcgg caatcaaaaa ggcgaggttc agagtgcccg tcactaccca gacgatcccg
    14881 gataacacca ccacctgtag gcccaactcc acgacgctgg cgatcagcat cgtcgcgcca
    14941 gcgccctgac ggtgagtgcg ggtctgtaag ttttccagcg cattgaaatg cgccagcagc
    15001 gcccggcttt tatcggcatc gctgccgcag gtgcgcaata ccatcatccc ctgagcaaat
    15061 tcaacgatat cgccgctcag acgctggtgc gcttccccca gcgtctgcat ttgtcgccgc
    15121 atcgccgggc ggcgccagta ataaaacggc accagcagag ggaagatcag caacatcacc
    15181 agccccagcc gccagtctat ccacaatgtc gccagcgacg ccgtcagcgg cgtgacaatg
    15241 gtgagcagca aaatattggc tatcgcaata acataattga ggttttcatc cacgctgccc
    15301 agcagcaagg cgttcatttc acccgccctg ccgcgctgga gcttctccag cggcacgcgg
    15361 cgtaactgct cgccaagtcg caggcgcaac tcatgggtag cctgcgccag atgaccacgg
    15421 tattcaaagc ccaggccata ccagcgtagc accagcgtca caattgcggc gacgctgaag
    15481 gccatagccc agttaagaag ttgcggcgcg tctccccgta acagcgcatc aatgatcgga
    15541 taaagacagg caaaggcgat cccctgcatg aatgccgcca gcaacagcgc cagcatactg
    15601 cgtcgcagca tcctggcctg actgccaacg ctggagataa gctggcgcca gatgacgcgc
    15661 caggccaggt tatccgcagg attattgtct ttcattttcc accacctctt cctgtgttcc
    15721 acccggcacc caatgctgcg cctgctgata gtgttgccag agccgttgat acaggccgcc
    15781 ctgcgccaac agttgcgtat ggttccccat ttccctgagc tgtccgtcgg aaaacagcaa
    15841 tatcacatcg gcctgagtca ccattgagag acgatgcgcg accatgatga ccgtccggcc
    15901 acgcatggcc gccgcgagcg ccttgataag cgccgcttcg ttttccgggt cagcaaacgc
    15961 cgtcgcctca tcgagcacca ggatggggcg atcctgcaaa agcgcccggg cgatagtaat
    16021 gcgctgccgc tggccgccgg agagaaatac cccacgttcc ccgactcgag tgttgtaacc
    16081 ttctggcaga gcgctaataa aatcatgggc ctgcgccact ctggccgccg ctataaccgc
    16141 ctccagcggc gtatccggcg cgcccagacg aatgttattg gctatcgtgt cggcaaaaag
    16201 gaagttgtcc tgaaacacaa acgagagttg cttcatcagg gtgtccgtct gcatatcacg
    16261 cagatccacg ccgccaatac ggatatggcc tttgtccggg tcggcgtaac gcagcagcaa
    16321 gcgcgccacg gtgctttttc cggcgccgct tggcccgacc agcgccacaa tttgcccggc
    16381 aggcacatga aagctcacct cctgcaacgc ggcgccagta cgcgcttgcg gataatgaaa
    16441 gctcacctgc tcaaaggtaa tgctcgcctc ttgcggctgc tggtcagact gcggcagcga
    16501 taactccggc atcgcgagaa cctgataaat acgctgaatg cttaaacgcg tttgcgcgac
    16561 caggttattg agcatcatca ttggcattac ggcttcggcc attccgctgc ccagcagtaa
    16621 aacggccacc cacgcgataa aatcgaaact gccatagtgc aacaggccgt atcccgacca
    16681 gatcaggaca aacagggtcg ggagaggatt cagaatcgag aaggaaaaac gcgctgaaaa
    16741 accggctttg cgataccagg ttttgagcac atcgacccac tcttcaaggg cgcgttgata
    16801 gcgcaaaaaa ctggtgctgc cgctatcaaa cgttctcacc acgggcatgg cctgcacgaa
    16861 ctcaataacc gccgcgctaa cctgctcgcg cgccttatga taacgctgag ccatatcttc
    16921 cgaacgctgg cgggcgagca cgagaacaac cgatccaaac gccagtaccc ccagcgtcgc
    16981 gattgccagc cgccagtcca gccagaacaa gataacgatt gtcgccagcg gcatgatgat
    17041 cgcgcgggca tagagcggcg tgctgtcggc cacaaaaata tgtaacgact tcacgtcatc
    17101 cagcatcact ttcgccactg acccgctgcc catttgctgt aacacgccag gcggaagctg
    17161 caatgcttta cgggctaacc tgctgcgcag aatgttctcc agatgaaaag ccgcgtaatg
    17221 agaggtgttg aacgcctgta agcgcagaac aaaagttaag accacgcagc cgatcacgcc
    17281 gcccagcggc caggccggaa tagcgtcagg cgtcgcgcga atgtcacgca ggctccacgc
    17341 cagaaaaagg agcgccccca ggctggttaa tgccgccagc cccgccagcg ccatagctaa
    17401 aataaccttc ctgcgaaccg gtgtaatcac ctgccagaga ggaaatcggg acagcgattc
    17461 agtattggat gattgagatg acatgggagt aactgaattt cctgatgaat ttttacggac
    17521 gccaaaaacg acaattcagg gtggcgttct gagaattaat gtgaataata accattatca
    17581 ataactcatc taccccattc ggttctattt atacccgcat tggtctaagc cacagggaga
    17641 taaccaggtc atgatggagt caccgcaaac gcaatctgaa atctctattc accagttggt
    17701 ggtcgggaaa cctgcaaacg atggcaatat tcctgctcag tgtgaactgc tgcgctgttc
    17761 gttgcaggaa ggtatggata ttttgctctg gaggggccat tttgcgcggc ctgagacgtt
    17821 gcagctacac gacgatctgg gcaggattaa cttctcgtgc atcctggaag gcacatcgcg
    17881 ctttgcgatt cagggactcc ggcgccacac cgactgggag cttgcgcgca atcgccatta
    17941 cattacccat acgccagact gtcgcggcag cgcctcctat tgtggccgat tcgagagcat
    18001 taccctctcc ttcagccctg aaaccctcgc gctgtgggtg ccggatatta gcgcggtcat
    18061 taagaataag atcgactccc actgctgttg ccagcagcag cgttgtaatg ctgaaaccca
    18121 cttaacggct caggccttgc gtcacgcgct gatgcgcatg cacggcggct gcagtcatga
    18181 acaaaagccg tcgacgctat ggttgcaggg gcaaagtctg gtcatgctca gtctggtgct
    18241 ggatgaacat cgcgaagacg ccagttgcct ctcctgccac ttcaacccga tggaacgcca
    18301 gaaactgctg cgggcgaagg atctgctgct ggctgatctg acgcaagcgc cgggcgtcgc
    18361 ggagctggcc agagagtccg gtctgagcgt tctcaaaatt aagcgaggct tccgcgttct
    18421 gttcaacaac agcgtctatg gcctgtttca ggctgaacga atgcaggaag ccagaagacg
    18481 cctggccaac ggcaatacct ccgtcatgac ggtggcggcc gatctgggct atgccaacgc
    18541 cagccatttc tctgccgcct tccagaaaca gtttggcgta actccttcga cctttaaacg
    18601 cgggatgtaa ccggggtcgc gcccccctaa actcctccct gacagaggcc cggatacccc
    18661 atccgggcct ctgttctttc cgcctgttac caacccgaaa cgggcataaa taaacccgtt
    18721 tcgggtagca tccaattgtt aataattatt attctcatat gagtaatgct tttcggtaag
    18781 acgtgccatc aggaggaaga atgatttctg gcgcaccatc tcaggattcg ctgttaccgg
    18841 acaaccgcca cgcggctgat taccaacaat tacgcgagcg gctcatacag gaactgaatt
    18901 taacgccgca gcagttacat gaagagagca acctgatcca ggccggcctg gattccataa
    18961 gattgatgag atggttacac tggtttcgta aaaatggcta ccgccttacc cttcgcgagc
    19021 tgtatgccgc ccccacgctg gcggcatgga accagttaat gctcagccgg tcgccggaga
    19081 acgcggaaga agaaacgccg cccgacgaat catcctggcc gaacatgacc gaaagtaccc
    19141 ccttcccatt gacgccagta cagcacgcct acctgacggg ccgcatgccg gggcagacgc
    19201 ttggcggcgt gggttgccac ctgtatcagg agtttgaagg ccattgtctg acggcgtcgc
    19261 agctggagca ggccatcacg accttgctgc aacgccaccc aatgctgcat atcgcctttc
    19321 gccccgacgg gcagcaggtc tggctaccgc aaccttactg gaacggcgtc accgttcatg
    19381 atttacgcca taacgacgct gaaagccgcc aggcctatct ggacgcactg cgccagcgcc
    19441 tgagccaccg tcttttacgc gtggaaatcg gcgaaacgtt tgattttcag ctgacgctct
    19501 tgccggacaa tcgccaccgc ctacatgtca atattgacct gctgattatg gatgcctcca
    19561 gctttacgct tttcttcgat gagcttaacg ccctgctggc cggagaatcg ctgccggcta
    19621 tcgacacccg ctatgatttc cgctcgtatt tgctgcacca gcagaagatc aatcaaccac
    19681 tgagagacga cgcgcgcgct tactggctgg cgaaagcatc gacgcttccc cccgcgcccg
    19741 tcttgccgct ggcctgcgaa cccgccacgc tacgtgaagt ccgtaatacc cgacgccgca
    19801 tgattgtccc ggcaacacgc tggcacgcct ttagcaaccg ggccggcgag tatggcgtga
    19861 cgccgacaat ggcactggcg acctgttttt ctgccgtgct ggctcgctgg ggcggcctga
    19921 cgcgtctgct gcttaacatc accttattcg accgccagcc gctgcaccca gcggttggcg
    19981 cgatgcttgc cgacttcacc aatattcttc tgctggatac cgcctgcgat ggcgataccg
    20041 tcagcaacct ggcgcgtaaa aaccagctca cgtttacgga ggactgggag catcgccact
    20101 ggtccggcgt cgaattactc cgtgaactca aacgccagca gcgctacccc cacggcgccc
    20161 cggtggtatt taccagcaat ctggggcgtt ccctctacag cagccgcgca gaatcgccgt
    20221 tgggcgagcc ggaatggggc atctcgcaaa cgccgcaggt ctggatagat catctggcgt
    20281 tcgagcatca cggcgaggtc tggctacaat gggacagcaa cgacgcgctg ttccctccgg
    20341 cgttagtcga aacattgttc gacgcctact gccagttgat taaccaactc tgcgatgacg
    20401 aaagcgcctg gcaaaagccg ttcgcagata tgatgcccgc cagccagcgc gcgatacgcg
    20461 aacgggtcaa cgccaccggc gcccccattc ccgaaggctt gctgcatgaa ggcattttcc
    20521 gtatcgctct gcaacagccg caggcgctgg cggtaacgga catgcgttat cagtggaatt
    20581 atcatgagct gacagactat gcccgccgtt gcgcgggcag gttaatcgag tgcggggttc
    20641 agcccggcga taatgtggct atcacgatgt cgaaaggcgc aggacaactt gttgcggttc
    20701 tggccgtcct gctggccggg gcggtttacg ttccggtttc gctggatcag cctgccgcac
    20761 ggcgcgagaa aatctacgct gacgccagcg tccggctggt gctcatttgt cagcacgacg
    20821 ccagcgccgg gtcagacgat attcccgccc ttgcctggca gcaggccatt gaggcggagc
    20881 cgatcgccaa cccggtagta cgcgccccca cgcaaccggc ctacattatc tacacctccg
    20941 gctctaccgg tacgccgaaa ggggtagtca tttctcaccg gggagcgctt aacacctgtt
    21001 gcgatatcaa tacccgctat caggttggcc cgcatgacag ggtgctggcc ctctccgccc
    21061 tacattttga tttatcggtt tacgacattt ttggcgtact gcgcgcgggc ggcgcgctgg
    21121 tgatggtgat ggaaaatcaa cggcgcgatc ctcacgcatg gtgtgagctg atccagcgcc
    21181 atcaggtcac gctctggaac agcgtcccgg cgctgttcga tatgctgctg acctggtgtg
    21241 aaggtttcgc cgacgccacg ccggaaaacc tgcgcgcagt gatgctttcc ggcgactgga
    21301 tcgggcttga cctccccgcc cgttatcggg ccttccggcc acaaggacaa tttatcgcga
    21361 tgggcggtgc caccgaggcg tctatctggt ctaacgcctg cgaaattcac gacgtccccg
    21421 cccactggcg ctccatccct tacggttttc cgctaaccaa ccaacgctac cgggtggtgg
    21481 atgaacaggg ccgggactgc cctgactggg tgccgggtga attatggatt ggcggcattg
    21541 gggtcgcgga aggctatttc aacgatcccc tgcgtagcga gcagcaattt ttgacgctcc
    21601 cggacgagcg ctggtatcgc accggcgatc tcggctgcta ctggccagat ggcacaatcg
    21661 agttcctcgg tcgtcgcgac aagcaggtca aagtcggagg atatcgcatc gagctgggcg
    21721 aaatcgaaag cgcgctcagc cagctggcgg gggtgaaaca agcaaccgtt ctggcgatcg
    21781 gcgaaaaaga aaaaacgctg gcggcatacg ttgttcctca gggcgaggct ttttgcgtta
    21841 ccgatcatcg gaacccggca ctgccgcagg cgtggcacac gcttgcggga acgttgccct
    21901 gttgcgccat ctcgccagag atctccgcag aacaggtagc cgatttcctt cagcatcgcc
    21961 tgctaaaact gaagccgggt cacaccgctg gcgccgatcc tctccccctg atgaactcac
    22021 tcgctatcca gccgcgctgg caggccgtgg tggaacgctg gttagcattt ctggtgacac
    22081 aacggcgact gaagcccgct gctgaaggtt atcaggtctg cgctggtgaa gaacgcgagg
    22141 atgagcaccc gcacttcagc ggacatgatt taacgttatc gcaaattctt cgcggtgccc
    22201 gtaacgaact gtcgttactg aacgacgcgc agtggtcgcc ggaaagcctg gcctttaacc
    22261 atccggccag cgccccgtat attcaggaac tggcgacaat ttgccaacag cttgcacagc
    22321 gcttacagcg cccggtacgc ctgcttgagg tgggaacccg cactggccgc gccgcagaat
    22381 cgctgttagc acagctcaac gccggacaga ttgagtatgt cgggcttgag cagagccagg
    22441 agatgctgct gagcgcccgg cagaggctcg ccccctggcc tggcgcccgt ctgtccctct
    22501 ggaatgcaga cacgctggcg gcgcacgctc actcggcgga cattatctgg cttaataacg
    22561 ccctgcatcg tctgctgccg gaagatcccg ggctccttgc gacattacaa cagcttgccg
    22621 ttcccggcgc gctgctctac gtgatggagt ttcgccagtt aacgccgtcc gccctactca
    22681 gcacgctcct gttaaccaat gggcagccgg aggccttgct gcataacagc gccgactggg
    22741 cggcattatt tagcgcggcc ggcttcaact gtcagcatgg cgatgaggtc gcggggttac
    22801 aacgcttcct cgtacaatgt cctgacaggc aggtgcgccg cgatccccgt caacttcagg
    22861 ccgccctcgc cgggcgtctg ccggggtgga tggtgccgca acggatcgtc ttcctcgacg
    22921 ccttaccgct gacggctaac gggaaaattg actaccaggc gctgaagcgt cgtcataccc
    22981 ctgaagcgga aaacccggcc gaagcggatt taccccaagg cgacattgaa aaacaggttg
    23041 ccgccctctg gcagcaactc ttatcaactg gcaatgtcac cagagaaacc gacttcttcc
    23101 agcaaggcgg cgatagcctg ctggcgaccc gtctgaccgg gcaacttcat caggcaggtt
    23161 atgaagcgca attaagcgac ctgtttaatc atccccggct ggcggatttt gccgccacgc
    23221 tgcggaaaac cgacgtcccg gtagaacaac cattcgtcca ctcccctgaa gatcgctacc
    23281 agccctttgc gcttaccgac gtgcagcagg cttacctggt ggggcgtcag ccgggctttg
    23341 ccctgggcgg cgtcggctca catttctttg ttgaatttga aattgccgat ctggatctca
    23401 cccggttgga gacggtctgg aaccgattaa tcgcccgcca cgatatgcta cgcgccgtcg
    23461 tgcgtgatgg acagcaacag gtgctcgaac agacgcctcg ctgggtaata cccgcacaca
    23521 tcctccatac gcctgaagag gcgttgcagg tgcgcgaaaa actggcccat caggtactca
    23581 accccgaagt ctggccggta ttcgatctcc aggtcggtta cgttgacggg atgcccgccc
    23641 gcctgtggct gtgtctggat aacctgttac ttgacgggct gagcatgcag attctgttgg
    23701 cggagctgga acatggctac caatatatcc gcaacagttg cctccgccgc tacccgtcac
    23761 ctacagggat tacctgcaac aacccgcgat ccagtcgctt aacgcagatt ctctggcatg
    23821 gtggcaggcg caacttgatg atattcctcc ggctcctgca ttgccgctgc gctgcttgcc
    23881 tcaggaggtt gaaacaccgc gcttcgctcg cctgaacgac gtactcgaca gcacacgctg
    23941 gcatcggctg aaaaaacggg cggctgacgc ccatctcacc ccgtcagccg tgctgttgtc
    24001 cgtgtggtca accgtgctct ctgcatggag cgcgcagcct gagttcacgc ttaatcttac
    24061 gcttttcgac aggcgaccac tgcacccgca aatcaaccag attctgggcg atttcacctc
    24121 gctgatgctg ctgagctggc atcccggcga aagctggctg cacagcgcgc agtcactaca
    24181 gcagcggctg agccagaacc tcaaccaccg cgatgtgtca gccatccgcg tgatgcgtca
    24241 actggcgcaa cggcaaaacg tgccagccgt tccgatgccc gtcgtcttta ccagcgcgct
    24301 gggctttgag caggataact tcctcgcccg gcgtaatctg ctcaaaccgg tctggggcat
    24361 ctcccagacg ccgcaggtct ggctcgatca ccagatttat gaatccgaag gcgaactgcg
    24421 ctttaactgg gattttgtcg ccgcgctgtt tcctgccggg caggtggagc gccagtttga
    24481 acagtattgc gcattgctaa accgaatggc cgaggatgaa agcggctggc aactgccgct
    24541 cgccgcgctg gtgcctcccg ttaaacacgc agggcaatgc gcagagcgct caccgcgcgt
    24601 atgccctgag cactctcagc cacacattgc ggcggacgag agcaccgtca gcctgatttg
    24661 cgacgccttc cgcgaggtgg ttggcgagtc tgtcacgccc gcagaaaact tctttgaggc
    24721 gggcgcaacg tcgctgaatc tggtgcaact gcacgttttg ttacaacgtc acgaattttc
    24781 caccctgacg ttgcttgacc tcttcaccca cccttctcct gctgccctgg ccgattatct
    24841 ggccggcgtc gccacggtgg agaaaacaaa acgacctcgc cctgttcgcc gtcgtcagcg
    24901 gcggatatcg cgcgaagcaa actgattttc cccggaacgc catcgcgaac gcatggcgtt
    24961 ccattgactt tgtgaatatt aggaaacggg accgattatg gataacttgc gcttctcttc
    25021 tgcgccgaca gcagattcca ttgatgcatc gatcgttcaa cactacccgg actgcgaacc
    25081 tgtcgcggtt atcggctacg cctgccattt tcctgaatcg ccggatggcg aaacgttctg
    25141 gcaaaatctg ctggaaggtc gtgaatgcag ccgacgcttt acgcgcgaag agcttctggc
    25201 cgtcggtctg gatgtcgcca tcattgacga tcctcattat gtcaatatcg gtacggtgtt
    25261 agacaacgcc gactgcttcg acgccaccct gtttggctat tcgcgacagg aagcggagtc
    25321 gatggacccg cagcagcgcc tgtttttgca ggcggtctgg catgcgctgg aacatgccgg
    25381 ttatgccccc ggcgccgtcc cccataagac cggcgttttc gcctcttccc ggatgagtac
    25441 ctaccccggt cgcgaagcat tgaacgtgac agaagtcgcg caggtaaaag gtctgcaatc
    25501 tctgatgggc aatgataaag actatattgc cacccgcgcc gcgtacaaac tcaacctgca
    25561 cggcccggcg ttatcggtac agaccgcctg ctccagctcg ctggttgccg tgcatctggc
    25621 ctgtgaaagc ctgcgcgcag gcgaatccga tatggcggtt gccggcggcg tggcgctctc
    25681 tttcccccag caggcaggct accgctacca gcccggaatg attttctctc ctgatggtca
    25741 ctgtcgtccc tttgacgcct cggctgaggg cacctgggcc ggtaacggtc tcggctgcgt
    25801 ggtgctgcgt cgcctgagag acgcgctgct gtcaggcgat ccgattatct cggtgatcct
    25861 ctccagcgcg gtcaacaacg acggcaacag aaaggtcggc tataccgccc cttccgtcgc
    25921 agggcaacag gcagtcatcg aagaggcgtt aatgctggcg gccatcgacg acaggcaggt
    25981 aggttacatt gaaacccacg gcaccggcac accgctgggc gacgcgattg aaattgaagc
    26041 gttacgcaac gtctatgcgc ctcgcccgca ggatcagcgc tgtgcgctcg gttccgtgaa
    26101 aagtaacatg ggccatctgg ataccgcggc gggcattgcc ggactgctga aaaccgttct
    26161 ggcagtcagt cgcgggcaaa ttcctccctt actgaatttt cacaccccca acccggcgct
    26221 gaaacttgaa gagagcccct ttaccatacc ggtgtcggca caggcatggc aggacgaaat
    26281 gcgctatgcg ggcgtctcct cctttggtat tggcggcacc aactgccata tgatcgtcgc
    26341 ctcgctgccc gacgcgctca acgcgcgcct ccccaatacg gatagcggca gaaaaagtac
    26401 cgcgctgctg ctcagcgccg ccagcgacag cgcgttgcgg cggctggcga cggattatgc
    26461 cggggcgctg agagagaatg cggatgccag ctctctggcc ttcacagccc tgcacgcgcg
    26521 ccgtctcgat cttcctttcc gcctggcggc gccattaaac cgtgaaaccg ccgaggcgct
    26581 cagcgcctgg gctagtgaga aatcgggggc gctggtttac agcggccacg gcgccagcgg
    26641 caagcaggtg tggctgttta ccggccaggg ctcgcactgg cgcactatgg gtcaaacgat
    26701 gtaccagcac tcaacggcgt ttgccgacac gctggatcgc tgtttttccg cctgtagcga
    26761 aatgctcacg ccgtcactgc gcgaagcgat gtttaacccc gattcggcgc agctggacaa
    26821 tatggcctgg gcgcagccgg cgattgtcgc gtttgaaatc gcgatggcgg cgcactggcg
    26881 tgctgaagga ctgaagccag acttcgccat tgggcattcc gtcggtgaat ttgccgctgc
    26941 cgttgtctgc ggacactata cgattgaaca ggtcatgcca ctggtttgtc ggcgcggcgc
    27001 gctaatgcag cagtgcgcaa gcggcgcgat ggtggcggta tttgcagacg aagacacgct
    27061 gatgccgctg gctcgccagt ttgagctgga tctcgccgcc aacaacggta cgcaacatac
    27121 ggtattttcc gggccggaag cccgtctcgc ggtattttgc gccacgctct cgcagcatga
    27181 cattaactat cgtcgcctga gcgtaaccgg tgcggcgcac tccgctttac tggagccgat
    27241 actcgatcgg ttccaggacg cctgcgcggg actgcacgcg gagccggggc aaataccgat
    27301 tatttccacg ctcaccgccg acgtcattga tgagtcaacg ctcaaccagg cggattactg
    27361 gcgccgacac atgcgccagc cggtgcgttt tatccagagt attcaggtgg cgcatcagct
    27421 cggcgcccgc gtttttctgg agatggggcc cgatgcccag ttggttgctt gcgggcagcg
    27481 cgaataccgc gataacgcat actggatagc cagcgcccgg cgtaacaaag aggcgagcga
    27541 tgtcctcaat caggccctgc tccagcttta cgctgccggc gtcgccctac cgtgggccga
    27601 cctgctggcg ggcgatggac aacgtatcgc tgcgccatgt tatccgtttg atactgagcg
    27661 ttactggaaa gagcgcgtct ccccggcctg cgagcctgcc gacgcagcgc tgtctgccgg
    27721 gctggaggtg gcgagtcgcg ccgcgacagc gctcgatctc cctcgcctgg aagcgcttaa
    27781 acagtgcgcc acgcgactgc acgccatcta cgtcgatcaa ctggtacaac gctgtaccgg
    27841 cgatgccatt gagaacggcg tggacgccat gaccatcatg cgccgtggac gtctgctgcc
    27901 ccgctaccag cagctactcc agcgcctgct gaataactgc gtggtcgacg gcgattaccg
    27961 ctgcaccgac gggcgatacg tccgcgcccg ccccattgaa catcaacagc gggaatcact
    28021 gctgacggaa cttgccggtt attgtgaagg ttttcaggct attcccgaca ccatcgcccg
    28081 tgccggcgat cggttatatg aaatgatgag cggcgcggaa gaaccagtgg cgattatctt
    28141 cccgcaaagc gcctccgacg gcgtggaagt gctgtatcag gaattcagct ttggccgcta
    28201 tttcaaccaa atcgccgccg gggtattacg cggcattgtc cagacgcgtc agccccgcca
    28261 gccgttgcgt attcttgaag ttggcggcgg aaccggcggc accaccgcgt ggctgctgcc
    28321 ggaactcaac ggcgttccgg cactggagta ccatttcacc gatatctcgg cgctgttcac
    28381 ccgtcgcgcc cagcagaaat tcgccgacta cgattttgtg aagtatagcg agctggatct
    28441 cgaaaaagag gcgcagtctc agggtttcca ggcacagtct tacgatctta tcgtggcagc
    28501 gaacgtgatt cacgccaccc gccatattgg ccgcacgctc gataatctgc gccccctgct
    28561 caagccgggc gggcgcctgc tgatgcgcga aatcacccag ccaatgcgtc tgtttgactt
    28621 cgttttcggc ccgctggttc ttccgctaca ggatctcgac gcccgcgaag gtgagttatt
    28681 cctcaccacc gctcagtggc aacaacagtg ccgccacgcc ggattcagca aagtggcgtg
    28741 gctaccgcag gatggcagcc cgaccgccgg gatgagcgaa catatcattc tcgccacgct
    28801 gcccggtcag gcggttagcg ccgtaacatt caccgcgcca tcagaacccg tgttggggca
    28861 ggcgctgacg gataacggtg attatctcgc cgactggtct gattgcgcag gtcagcccga
    28921 acagtttaac gcccgctggc aggaggcatg gcgtctgctt tcacagcgtc atggcgacgc
    28981 tctgcctgtg gaaccgcccc ccgtcgccgc cccggagtgg ctggggaagg ttcgcttaag
    29041 ctggcaaaac gaagcctttt cccgcggtca gatgcgcgtt gaagcccgtc atcctgctgg
    29101 cgagtggctg ccgctatcgc ccgccgcgcc tcttcctgcg ccgcaaacgc attatcaatg
    29161 gcgctggacg cccctcaacg tcgccagcat tgaccatccg cttaccttta gcttcagcgc
    29221 cggtacgctt gcgcgcagcg acgagctggc gcaatacggc atcattcacg atccgcacgc
    29281 ctcttcacga ctgatgattg ttgaggagag cgaggatacg ctggccttag cggagaaagt
    29341 gatagcagcg ctcaccgcca gcgcagccgg attgattgtg gttactcgcc gcgcgtggcg
    29401 agtcgaggaa aatgaagcac tctctgcatc ccatcacgcg ctatgggcct tgcttcgcgt
    29461 cgcggccaac gaacagccgg aacggttgct tgccgccatc gatctcgccg aaaacacccc
    29521 gtgggaaacg ctgcatcaag ggttgagcgc agtctcacta tcacagcgct ggctcgccgc
    29581 acggggtgac accctttggc tcccttcact agcgcccaat acgggatgcg ccgctgaatt
    29641 accggcaaac gtgtttaccg gcgatagccg ctggcatctg gtgaccggag cgtttggcgg
    29701 attaggccgc cttgccgtga actggctcag agaaaaaggg gcgcgacgca tcgccctgct
    29761 ggcgccgcgc gtggatgagt catggctacg cgacgtggag ggcgggcaga cgcgcgtctg
    29821 ccgttgtgat gtgggcgatg ccgggcaact ggccacggtt cttgacgatc tggcggccaa
    29881 cggcggcatt gccggagcga ttcatgccgc tggcgtattg gctgacgcgc ccttgcagga
    29941 gcttgatgac caccagctgg ctgccgtttt cgcggtaaaa gcgcaggcgg caagccagct
    30001 gttgcaaacc ctgcgcaacc acgacggacg ctatcttatt ctctactctt ccgctgccgc
    30061 caccctcggc gcgccgggtc agagcgccca tgcgctggcc tgcggctacc tggacgggct
    30121 ggcccagcag ttttccaccc ttgatgcgcc gaaaacgctc tctgtcgcct ggggcgcatg
    30181 gggagaaagc ggtcgggcgg ccacgccgga aatgctggcg acgctcgcca gccgaggtat
    30241 gggcgcgtta agcgatgccg aaggctgctg gcacctggaa caggcggtga tgcgcggcgc
    30301 cccgtggcga ctggcgatgc gcgtttttac cgacaaaatg cccccgttac aacaggctct
    30361 gtttaacatc agcgccacag aaaaagccgc aacgccggtc attcctcctg ctgatgacaa
    30421 cgcctttaac ggcagcctga gcgatgaaac agcggtgatg gcatggctga aaaagcggat
    30481 tgcggttcag ctaaggctga gcgatccggc gtcactgcat ccaaaccagg atctgttgca
    30541 actcggcatg gactcgctgc tcttccttga actcagtagc gatattcagc actacctggg
    30601 cgtacgcatc aatgcggaac gggcgtggca ggatctgtct cctcatggac tcacgcagct
    30661 tatctgttct aagccagagg cgacgcctgc cgcttcgcag ccggaagtgt tgcggcacga
    30721 cgccgacgag cgttatgcgc ccttcccttt gacgcccatt cagcacgcct actggctggg
    30781 gcgaacccac ctcattggct atggcggcgt cgcctgtcac gtcctgtttg agtgggataa
    30841 acgccacgat gagttcgatc tcgccatact ggagaaagca tggaaccagc tcatcgcacg
    30901 ccacgatatg ttgcgtatgg tggttgatgc cgacgggcag cagcgaatcc tggcgacaac
    30961 gccggagtat cacatcccgc gtgacgatct gcgcgcgctt tccccggaag aacagcgcat
    31021 cgcgctggaa aaacggcggc atgaactgag ctatcgcgtt ttgcctgccg accagtggcc
    31081 tctttttgag ctggtggtca gcgaaatcga cgattgccat taccgtctgc atatgaacct
    31141 cgaccttttg cagtttgatg tgcagagttt taaagtcatg atggacgacc tggcgcaggt
    31201 ctggcgcggt gaaacgctgg cgccgctcgc tattaccttc cgtgattatg tgatggctga
    31261 acaggcgcgc cgacagacat cggcatggca cgatgcctgg gattactggc aggaaaaact
    31321 gccgcaactg cccttagcgc cagagctgcc ggtggttgag acgcccccgg aaacgccaca
    31381 cttcaccacc ttcaaatcga cgatcggcaa gacagaatgg caggccgtga aacagcgctt
    31441 gcagcagcaa ggcgtcacac cgtctgccgc gctgctcacg ctgtttgccg ccacccttga
    31501 gcgctggagc cgtaccacaa catttacgct gaacctgacg ttcttcaatc gccagccgat
    31561 ccatccgcaa atcaaccagt tgattggtga ttttacctcc gtcacgctgg ttgattttaa
    31621 cttctcagcg ccggtgacgt tgcaagagca gatgcaacag acccaacagc gcctctggca
    31681 aaacatggcg cacagtgaaa tgaacggtgt tgaggtgatc cgtgagctgg gccgcctgcg
    31741 cggatcacaa cgtcaaccgc tgatgccggt agtgtttacc agtatgctgg ggatgacgct
    31801 ggaaggcatg actatcgatc aggcgatgag ccatctgttc ggcgaaccct gctatgtatt
    31861 cacgcaaacg ccgcaggtct ggctggatca tcaggtcatg gagagcgacg gcgagttgat
    31921 gtttagctgg tactgcatgg acaacgtgct ggaacccggc gctgccgagg cgatgtttaa
    31981 tgactattgc gccatcctgc aagccgtcat cgccgcccct gaaagcctga agactctcgc
    32041 cagcggcatc gccgggcaca ttccccgccg acgctggccg ctgaacgcgc aggcggacta
    32101 cgacctgcgg gatattgagc aggcgacgct cgaatacccc ggcatccggc aggccagagc
    32161 ggaaataacc gaacagggcg cgttgacgct ggatatcgtg atggccgacg atccttcgcc
    32221 atcagcggcg atgcctgatg agcacgaact tacccaactg gcgctgccgt tgcctgagca
    32281 ggcgcagctt gatgagctgg aggcgacctg gcgctggctg gaggcgcgtg cgctacaggg
    32341 gatcgcggct acgctaaatc gtcacggcct gtttaccacg ccggagatcg cccatcgctt
    32401 tagcgcagta gtacaggcgc tgtccgcgca agcgtctcac cagcgtctgc tgcgccagtg
    32461 gctacagtgt ctgacggaaa gagagtggtt aatccgcgaa ggtgaaagct ggcgctgccg
    32521 cattccgctc agcgagattc ctgagcctca ggaagcgtgc ccgcaaagcc aatggagcca
    32581 ggcgctggcg cagtatctgg aaacctgcat cgcccggcac gacgccctct tctccgggca
    32641 gtgttctccg ctggaattgc tgttcaacga gcagcatcgc gttaccgacg cgctgtatcg
    32701 cgacaacccc gccagcgcct gtctgaatcg ctataccgcg cagattgccg ccttgtgcag
    32761 cgcagaacgg attctggagg ttggcgccgg aaccgcagcc actaccgcgc cggtgctgaa
    32821 ggccacgcgg aacacgcggc agtcgtacca cttcacggac gtctccgcgc agttcctcaa
    32881 tgacgccaga gcccgtttcc atgatgaatc gcaggtgtct tatgccttgt tcgacatcaa
    32941 ccagccgctg gatttcaccg cccacccgga ggcgggttac gacctgatcg ttgccgtcaa
    33001 tgtgctccac gacgccagcc atgtcgtcca gacgttgcgc agattaaaac tgttgctgaa
    33061 agccggcgga cgtttgctga tcgttgaagc gacggagcga aacagcgtat tccagctggc
    33121 gagcgtgggc tttattgagg gattaagcgg ataccgcgat ttccgccgcc gggatgagaa
    33181 accgatgctc acccgctccg catggcagga ggttctcgtt caggccgggt ttgcaaacga
    33241 gctggcgtgg cccgcgcagg aatcgtcgcc gctgcgccag catctgctgg tagcacgttc
    33301 gcctggcgta aatcgcccgg ataaaaaagc cgtgagccgc tatttacagc agcgctttgg
    33361 caccggtctg cccattttac agatccggca aagagaagcg ttatttacgc cgctgcatgc
    33421 cccgtctgat gcgccgactg agccagccaa acccacgcca gttgccgggg ggaatccggc
    33481 gctggaaaaa caggtggctg aactctggca atcgctgctg tctcgccccg tggcaaggca
    33541 tcacgacttt ttcgaactgg gcggcgacag cctgatggcg acaaggatgg tcgcgcagct
    33601 aaaccggaga gggattgcca gggctaacct tcaggatctg ttcaaccatt cgacgctgag
    33661 cgacttctgc gcccatctac aggcggctac gtcaggagag gacaacccga tacccctttg
    33721 ccagggcgac ggtgaggaaa ccctgtttgt cttccacgct tcagacggcg atatcagcgc
    33781 ctggctgccg ctcgctagcg cgttgaacag gcgcgttttc ggcctgcaag caaaatcgcc
    33841 gcagcgcttt gccacgctcg accagatgat cgatgagtat gtcgggtgca tccgtcgtca
    33901 gcagcctcac ggcccttatg tgctggcggg ttggtcgtat ggcgcgtttc ttgcggcggg
    33961 cgccgcacag cgcctgtacg ccaaaggcaa gcaggttcgg atggtgttaa tcgatcccgt
    34021 gtgccgacag gatttctgtt gcgcaaaccg ggcggccctg ctgcgcctgt tagccgaagg
    34081 acaaacgcct ctggcactgc ccgaacattt cgaccagcag acgcccgaca gccagcttgc
    34141 cgactttatc agcctcgcta aaacggccgg tatggtgtcg caaaacctga cgctgcaagc
    34201 ggcagaaacg tggctcgaca acatcgcgca tctgctgcgt ttactgactg agcatacgcc
    34261 gggcgaaagc gttccggtcc cctgtctcat ggtgtatgcc gccgggagac ccgcgcactg
    34321 gacgccagca gaaaccgagt ggcagggctg gataaacaac gccgacgacg ctgtgattga
    34381 agccagccac tggcaaatca tgatggaagc cccccacgtt caggcttgtg cgcaacacat
    34441 tacgcgctgg ctttgcgcaa cctcaacgca accggagaac acgttatgat gccgtccgcc
    34501 tccccaaaac aacgcgtact gattgtgggc gccaaatttg gcgaaatgta cctgaatgcc
    34561 tttatgcagc ccccggaggg gctggaactg gtcggcctgc tggcgcaggg aagcgcccgt
    34621 tcaagagagc tggctcatgc gtttggcatt ccgctgtata cctcgccgga acagataacc
    34681 aggatgccgg atatcgcctg tattgtcgtg cgttcgaccg tcgccggagg aaccggtacg
    34741 cagcttgcca gacactttct gacgcgcggc gtacacgtca ttcaggagca tcccctccac
    34801 ccggatgaca tcagttcctt acaaacgctg gcgcaggaac agggctgctg ctattgggta
    34861 aacacgtttt atccgcatac gcgcgcggga cgcacatggc ttcgcgatgc gcagcagctg
    34921 cgtcgctgtc tggctaagac gccgcaggtg gtccacgcca ccaccagccg acagttgctc
    34981 tactccactc tggatttgtt gttgctggct ctgggcgttg atgccgccgc cgtggagtgc
    35041 gacgtggttg gcagctttag cgattttcac tgtctgagac tcttctggcc ggagggagaa
    35101 gcctgcctgc tgcttcagcg ctatctcgat cctgacgatc cagatatgca tagccttatc
    35161 atgcaccgcc tgctgctggg ctggccggaa ggtcatcttt cgctggaggc gagctacggc
    35221 cccgtcatct ggtcatccag cctgtttgtc gcagaccatc aggagaacgc ccacagcctc
    35281 taccgcagac cggagatcct gcgcgatctg ccgggtctga cgcgcagcgc ggcgcccctg
    35341 agctggcgcg actgctgcga aaccgtcggg ccggagggcg tcagctggtt gctacaccag
    35401 ttgcgcagcc atctggcggg cgaacatcca ccagcggcct gccaaagcgt acatcagatc
    35461 gcgttatcac gtttatggca gcagatttta cgtaagaccg gcaatgcgga gatcagacgc
    35521 ctgacgccgc cgcaccacga ccggttagcc ggtttctaca acgatgatga taaggaggcg
    35581 ctgtgacgca atctgcaatg tgcataccgc tgtggcccgc ccggaacggc aatactgcgc
    35641 atctggtcat gtgccctttc gctggcggca gcagtagcgc gtttcgccac tggcaagctg
    35701 agcaactggc tgattgcgcg ctttctctgg tgacctggcc ggggcgcgat cgccttcgcc
    35761 atctggaacc gctcagaagc attacacaac tggcggcact gctggcgaac gagctggaag
    35821 catccgtatc gcctgacacg ccgcttttac tcgccgggca cagcatgggg gcgcaggtgg
    35881 cgtttgaaac ctgccgactt ctggagcaac gggggcttgc gccacaagga ctgattattt
    35941 ccgggtgcca tgccccgcat ctgcattctg aacgccagct cagccatcgc gatgatgccg
    36001 actttatcgc tgagctgata gacattggcg gatgttctcc tgaactgcgg gaaaaccagg
    36061 aattaatgtc gctgtttctt cctcttctgc gcgctgattt ttacgccacc gagagctatc
    36121 actacgactc gcccgacgtc tgtccgccgc tgcgcacgcc tgcgctgtta ttgtgcggca
    36181 gccacgatcg cgaagcctcc tggcagcagg tcgatgcctg gcgtcagtgg ctgagccacg
    36241 ttacaggccc ggtggtgatt gacggcgatc acttctatcc cattcaacaa gcccggtcct
    36301 tttttacgca gattgtccgc cattttcccc acgcattttc tgcaatgacc gcgtggcaaa
    36361 aacagcccag tacttcagaa aggtgacgca tgaattcttc ctttgaatct ctgattgaac
    36421 agtatccctt acccattgcc gaacagttgc gccactgggc ggcccgttat gcctcgcgaa
    36481 ttgccgtcgt tgatgcaaag gggtcgttaa cctacagcgc gcttgatgca caagttgacg
    36541 aacttgccgc aggtctgtca tcactgggtt tgcgttcggg ggagcatgta attgtgcagc
    36601 ttcccaatga caacgcgttt gtgaccctgc tgttcgcctt gttaagactg ggcgttatcc
    36661 ccgtgctggc gatgccctcg caacgggcgc tggatatcga cgcgctgatt gagctggcgc
    36721 aacccgtcgc ttacgttatt cacggggaaa accacgcaga gctggcccga cagatggcgc
    36781 acaaacacgc ctgcttgcgt catgttctgg tcgctggaga gaccatgagc gacgatttta
    36841 cgccgctctt ctcccttcac ggtgagcgac aggcatggcc gcagcctgat gtttccacca
    36901 ccgcgttgtt gttgctctca ggcggcacaa ccggcacgcc caaactcatc ccgcgccgac
    36961 atgccgacta tagctataac ttcagcgctt ctgctgaact gtgcggcatc agccaacaga
    37021 gcgtgtatct cgccgtcctc ccggtggcgc ataactttcc gctggcctgc cccggtattc
    37081 tgggaacgct tgcctgcggc ggaaaagtgg tgctgaccga cagcgccagc tgtgatgagg
    37141 tgatgccttt aatcgcgcag gaaagagtga ctcacgtcgc cctggttccg gcgctggcgc
    37201 aattatgggt gcaggccagg gagtgggaag acagcgacct ttcgtcgctg cgcgtcattc
    37261 aggcaggcgg cgcccggctc gacccgacgc ttgctgagca ggttatcgcc acctttgact
    37321 gtaccctgca acaggttttc ggtatggcgg aaggcctgct ctgttttacc cggctggacg
    37381 atccgcatgc caccattctc cacagccagg ggcgcccgtt gtcccctctg gatgaaatcc
    37441 gcatcgttga tcaagacgag aacgacgtcg cgccgggcga aaccgggcaa ttgttaacgc
    37501 gcggccctta taccatttcg ggctattacc gcgcccctgc ccacaacgcg caggccttta
    37561 ccgcgcaagg gttttaccgc acaggcgaca atgtcaggct ggatgaggtg gggaacctgc
    37621 acgttgaggg acgcataaaa gagcagatca accgcgccgg agaaaaaata gccgcggctg
    37681 aagtggaatc ggcactgctg cgtttagcgg aagtgcaaga ttgcgcggtg gtcgccgcgc
    37741 cggacacgct gcttggcgag cggatttgcg cgtttatcat cgcgcagcag gtgccaactg
    37801 actatcagca gttgcgtcaa caactgaccc gtatggggct cagcgcgtgg aaaattcctg
    37861 accaaatcga gtttctggac cactggccgc tcaccgccgt cggcaagata gacaaaaaac
    37921 gcctgacggc tctcgccgtc gaccgttatc gccattctgc ccaataagcg caaaccgacc
    37981 cgaaacaggt tgaaataaac ccgtttcggg tagcactact attagaaata gttatcattt
    38041 tcaattcacc attgtcggta tttttggcgt ttcgccgtct tacagggact cacaacaatg
    38101 aaaatgacac ggctttatcc tctggccttg gggggattat tgctccccgc cattgctaat
    38161 gctcagactt cacagcaaga cgaaagcacg ctggtggtta ccgccagtaa acaatcttcc
    38221 cgctcggcat cagccaacaa cgtctcgtct actgttgtca gcgcgccgga attaagcgac
    38281 gccggcgtca ccgccagcga caaactcccc agagtcttgc ccgggctcaa tattgaaaat
    38341 agcggcaaca tgcttttttc gacgatctcg ctacgcggcg tctcttcagc gcaggacttc
    38401 tataaccccg ccgtcaccct gtatgtcgat ggcgtccctc agctttccac caacaccatc
    38461 caggcgctta ccgatgtgca aagcgtggag ttgctgcgag gcccacaggg aacgttatat
    38521 ggcaaaagcg ctcagggcgg gatcatcaac atcgtcaccc agcagccgga cagcacgccg
    38581 cgcggctata ttgaaggcgg cgtcagtagc cgcgacagtt atcgaagtaa gttcaacctg
    38641 agcggcccca ttcaggatgg cctgctgtac ggcagcgtca ccctgttacg ccaggttgat
    38701 gacggcgaca tgattaaccc cgcgacggga agcgatgact taggcggcac ccgcgccagc
    38761 atagggaatg tgaaactgcg tctggcgccg gacgatcagc cctgggaaat gggctttgcc
    38821 gcctcacgcg aatgtacccg cgccacccag gacgcctatg tgggatggaa tgatattaag
    38881 ggccgtaagc tgtcgatcag cgatggttca ccagacccgt acatgcggcg ctgcactgac
    38941 agccagaccc tgagtgggaa atacaccacc gatgactggg ttttcaacct gatcagcgcc
    39001 tggcagcagc agcattattc gcgcaccttc ccttccggtt cgttaatcgt caatatgcct
    39061 cagcgctgga atcaggatgt gcaggagctg cgcgccgcaa ccctgggcga tgcgcgtacc
    39121 gttgatatgg tgtttgggct gtaccggcag aacacccgcg agaagttaaa ttcagcctac
    39181 gacatgccga caatgcctta tttaagcagt accggctata ccaccgctga aacgctggcc
    39241 gcatacagtg acctgacctg gcatttaacc gatcgttttg atatcggcgg cggcgtgcgc
    39301 ttctcgcatg ataaatccag tacacaatat cacggcagca tgctcggcaa cccgtttggc
    39361 gaccagggta agagcaatga cgatcaggtg ctcgggcagc tatccgcagg ctatatgctg
    39421 accgatgact ggagagtgta tacccgtgta gcccagggat ataaaccttc cgggtacaac
    39481 atcgtgccta ctgcgggtct tgatgccaaa ccgttcgtcg ccgagaaatc catcaactat
    39541 gaacttggca cccgctacga aaccgctgac gtcacgctgc aagccgcgac gttttatacc
    39601 cacaccaaag acatgcagct ttactctggc ccggtcggga tgcagacatt aagcaatgcg
    39661 ggtaaagccg acgccaccgg cgttgagctt gaagcgaagt ggcggtttgc gccaggctgg
    39721 tcatgggata tcaatggcaa cgtgatccgt tccgaattca ccaatgacag tgagttgtat
    39781 cacggtaacc gggtgccgtt cgtaccacgt tatggcgcgg gaagcagcgt gaacggcgtg
    39841 attgatacgc gctatggcgc actgatgccc cgactggcgg ttaatctggt cgggccgcat
    39901 tatttcgatg gcgacaacca gttgcggcaa ggcacctatg ccaccctgga cagcagcctg
    39961 ggctggcagg cgactgaacg gatgaacatt tccgtctatg tcgataacct gttcgaccgt
    40021 cgttaccgta cctatggcta catgaacggc agcagcgccg tcgcgcaggt caatatgggt
    40081 cgcaccgtcg gtatcaatac gcgaattgat ttcttctgat tattgtaaaa gggataccga
    40141 aaaggtatcc cttttacacc actagttaaa accagcaact cagcagagtc gcaaaaaata
    40201 ttaatccata gtgattattt aaacaatgaa attgcgatta ggataaatag atttaacttt
    40261 ctcgttcctt tctctcctta tactaaagaa ataatcatat caaaataaaa
//
LOCUS       DADSYM010000021        39763 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2415
            SAMN08039579-rid11723173.denovo.021, whole genome shotgun sequence.
ACCESSION   DADSYM010000021 DADSYM010000000
VERSION     DADSYM010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039579
            Sequence Read Archive: SRR6362533
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 39763)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39763)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980162.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 03:39:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,484
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 4,347
            CDSs (with protein)               :: 4,347
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 43 of 85
            Pseudo Genes (incomplete)         :: 37 of 85
            Pseudo Genes (internal stop)      :: 19 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39763
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039579-rid11723173.denovo.021"
                     /strain="LGE2415"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8302 N 95.9628 W"
                     /collection_date="2015-08-24"
     gene            <1..1724
                     /gene="adeD"
                     /locus_tag="J1Y83_000935"
     CDS             <1..1724
                     /gene="adeD"
                     /locus_tag="J1Y83_000935"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HBA7629107.1"
                     /translation="EYQELLAVSRGDAVADYIIDNVSILDLINGGEISGPIVIKGRYI
                     AGVGAEYADAPALQRIDARGATAVPGFIDAHLHIESSMMTPVTFETATLPRGLTTVIC
                     DPHEIVNVMGEAGFAWFARCAEQARQNQYLQVSSCVPALEGCDVNGASFTLEQMLAWR
                     DHPQVTGLAEMMDYPGIISGQNALLDKLDAFRHLTLDGHCPGLGGKELNAYIAAGIEN
                     CHESYQLEEGRRKLQLGMSLMIREGSAARNLNALAPLINEFNSPQCMLCTDDRNPWEI
                     AHEGHIDALIRRLIEQHNVPLHVAYRVASWSTARHFGLNHLGLLAPGKQADIVLLSDA
                     RKVTVQQVLVKGEPIDAQTLQAEESARLAQSAPPYGNTIDRQPVSASDFALQFTPGKR
                     YRVIEVIHNELITHSRSSVYSENGFDRDDVCFIAVLERYGQRLAPACGLLGGFGLNEG
                     ALAATVSHDSHNIVVIGRSAEEMALAVNQVIQDGGGLCVVRNGQVQSHLPLPIAGLMS
                     TDTAQSLAEQIDTLKAAARECGPLPDEPFIQMAFLSLPVIPALKLTSQGLFDGEKFAF
                     TTLEVTE"
     gene            complement(1771..3162)
                     /gene="uhpT"
                     /locus_tag="J1Y83_000936"
     CDS             complement(1771..3162)
                     /gene="uhpT"
                     /locus_tag="J1Y83_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexose-6-phosphate:phosphate antiporter"
                     /protein_id="HBA7629108.1"
                     /translation="MLAFLNQVRKPTLDLPLEVRRKMWFKPFMQSYLVVFIGYLTMYL
                     IRKNFNIAQNDMISTYGLSMTQLGMIGLGFSITYGVGKTLVSYYADGKNTKQFLPFML
                     ILSAICMLGFSASMGSGSVSLFLMIAFYALSGFFQSTGGSCSYSTITKWTPRRKRGTF
                     LGFWNISHNLGGAGAAGVALFGANYLFDGHVIGMFIFPSIIALIVGFIGLRYGSDSPE
                     SYGLGKAEELFGEEISEEDKETESTDMTKWQIFVEYVLKNKVIWLLCFANIFLYVVRI
                     GIDQWSTVYAFQELKLSKAVAIQGFTLFEAGALVGTLLWGWLSDLANGRRGLVACIAL
                     ALIIATLGVYQHASNQYIYLASLFALGFLVFGPQLLIGVAAVGFVPKKAIGAADGIKG
                     TFAYLIGDSFAKLGLGMIADGTPVFGLTGWAGTFAALDIAAIGCICLMAIVAVMEERK
                     IRREKKIQQLTVA"
     gene            complement(3300..4619)
                     /gene="uhpC"
                     /locus_tag="J1Y83_000937"
     CDS             complement(3300..4619)
                     /gene="uhpC"
                     /locus_tag="J1Y83_000937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312631.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter family glucose-6-phosphate
                     receptor UhpC"
                     /protein_id="HBA7629109.1"
                     /translation="MLPFLKAPADAPLMTDKHEIDARYRYWRRHILLTIWLGYALFYF
                     TRKSFNAAVPEILANGVLSRSDIGLLATLFYITYGVSKFVSGIVSDRSNARYFMGIGL
                     IATGIINILFGFSTSLWAFAVLWVLNAFFQGWGSPVCARLLTAWYSRTERGGWWALWN
                     TAHNVGGALIPIVMAASALHYGWRAGMMIAGCMAIVVGIFLCWRLRDRPQALGLPAVG
                     EWRHDALEIAQQQEGAGLTRKEILTKYVLLNPYIWLLSFCYVLVYVVRAAINDWGNLY
                     MSETLGVDLVTANTAVTMFELGGFIGALVAGWGSDKLFNGNRGPMNLIFAAGILLSVG
                     SLWLMPFASYVMQATCFFTIGFFVFGPQMLIGMAAAECSHKEAAGAATGFVGLFAYLG
                     ASLAGWPLAKVLDTWHWSGFFVVIAIAAGISALLLLPFLNAQTPREA"
     gene            complement(4629..6131)
                     /gene="uhpB"
                     /locus_tag="J1Y83_000938"
     CDS             complement(4629..6131)
                     /gene="uhpB"
                     /locus_tag="J1Y83_000938"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312632.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal transduction histidine-protein
                     kinase/phosphatase UhpB"
                     /protein_id="HBA7629110.1"
                     /translation="MKTLFSRLITVIACFFIFSAAWFCLWSISLHLVERPDMAVLLFP
                     FGLRLGLMLQCPRGYWPVLLGAEWLLIYWLMQAVGLTHFSLLMIGSLLTLLPVALISR
                     YRHQRDWRTLLLQGAALTAAALLQSLPWLWHGKESWNALLLTLTGGLTLAPICLVFWH
                     YLANNTWLPLGPSLVSQPINWRGRHLVWYLLLFVISLWLQLGLPDELSRFTPFCLALP
                     IIALAWHYGWQGALIATLMNAIALIASQTWRDHPVDLLLSLLVQSLTGLLLGAGIQRL
                     RELNQSLQKELARNQHLAERLLETEESVRRDVARELHDDIGQTITAIRTQAGIVQRLA
                     ADNASVKQSGQLIEQLSLGVYDAVRRLLGRLRPRQLDDLTLEQAIRSLMREMELEGRG
                     IVSHLEWRIDESALSENQRVTLFRVCQEGLNNIVKHADASAVTLQGWQHDERLMLVIE
                     DDGSGLPPDSGQHGFGLTGMRERVTALGGTLTISCLHGTRVSVSLPQRYV"
     gene            complement(6131..6721)
                     /gene="uhpA"
                     /locus_tag="J1Y83_000939"
     CDS             complement(6131..6721)
                     /gene="uhpA"
                     /locus_tag="J1Y83_000939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator UhpA"
                     /protein_id="HBA7629111.1"
                     /translation="MITVALIDDHLIVRSGFAQLLGLEPDLQVVAEFGSGREALAGLP
                     GRGVQVCICDISMPDISGLELLSQLPKGMATIMLSVHDSPALVEQALNAGARGFLSKR
                     CSPDELIAAVHTVATGGCYLTPDIAIKLASGRQDPLTKRERQVAEKLAQGMAVKEIAA
                     ELGLSPKTVHVHRANLMEKLGVSNDVELARRMFDGW"
     gene            complement(6794..7084)
                     /gene="ilvN"
                     /locus_tag="J1Y83_000940"
     CDS             complement(6794..7084)
                     /gene="ilvN"
                     /locus_tag="J1Y83_000940"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HBA7629112.1"
                     /translation="MQNTTHDNVILELTVRNHPGVMTHVCGLFARRAFNVEGILCLPI
                     QDSDKSHIWLLVNDDQRLEQMISQIDKLEDVVKVQRNQSDPTMFNKIAVFFQ"
     gene            complement(7088..8776)
                     /gene="ilvB"
                     /locus_tag="J1Y83_000941"
     CDS             complement(7088..8776)
                     /gene="ilvB"
                     /locus_tag="J1Y83_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase large subunit"
                     /protein_id="HBA7629113.1"
                     /translation="MASSGTTSTRKRFTGAEFIVHFLELQGIKIVTGIPGGSILPVYD
                     ALSQSTQIRHILARHEQGAGFIAQGMARTDGKPAVCMACSGPGATNLVTAIADARLDS
                     IPLICITGQVPASMIGTDAFQEVDTYGISIPITKHNYLVRHIEELPQVMSDAFRIAQS
                     GRPGPVWIDIPKDVQTAVFEIETQPAMAEKAAAPAFSEESIRDAAAMINAAKRPVLYL
                     GGGVINAPARVRELAEKAQLPTTMTLMALGMLPKAHPLSLGMLGMHGVRSTNYILQEA
                     DLLIVLGARFDDRAIGKTEQFCPNAKIIHVDIDRAELGKIKQPHVAIQADVDDVLAQL
                     IPLVEAQPRAEWHQLVADLQREFPCPIPKACDPLSHYGLINAVAACVDDNAIITTDVG
                     QHQMWTAQAYPLNRPRQWLTSGGLGTMGFGLPAAIGAALANPDRKVLCFSGDGSLMMN
                     IQEMATASENQLDVKIILMNNEALGLVHQQQSLFYKQGVFAATYPGKINFMQIAAGFG
                     LETCDLNNEADPQAALQEIINRPGPALIHVRIDAEEKVYPMVPPGAANTEMVGE"
     gene            complement(8882..8980)
                     /gene="ivbL"
                     /locus_tag="J1Y83_000942"
     CDS             complement(8882..8980)
                     /gene="ivbL"
                     /locus_tag="J1Y83_000942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ilvB operon leader peptide IvbL"
                     /protein_id="HBA7629114.1"
                     /translation="MTTSMFNAKLLPTAPSAAVVVVRVVVVVGNAP"
     gene            9381..10565
                     /gene="emrD"
                     /locus_tag="J1Y83_000943"
     CDS             9381..10565
                     /gene="emrD"
                     /locus_tag="J1Y83_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418129.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter EmrD"
                     /protein_id="HBA7629115.1"
                     /translation="MKRHRNVNLLLMLVLLVAVGQMAQTIYIPAIADMARDLNVREGA
                     VQSVMGAYLLTYGVSQLFYGPISDRVGRRPVILVGMSIFMLATLVAVTTSSLMVLIAA
                     SAMQGMGTGVGGVMARTLPRDLYERTQLRHANSLLNMGILVSPLLAPLIGGLLDTMWN
                     WRACYLFLLVLCAGVTFSMARWMPETRPVDAPRTRLLTSYKTLFGNSGFNCYLLMLIG
                     GLAGIAAFEACSGVLMGAVLGLSSMTVSILFILPIPAAFFGAWFAGRPNKRFSTLMWQ
                     SVICCLLAGLLMWIPDWFGVMNVWTLLVPAALFFFGAGMLFPLATSGAMEPFPFLAGT
                     AGALVGGLQNIGSGVLASLSAMLPQTGQGSLGLLMTLMGLLIVLCWLPLATRMSHQGQ
                     PV"
     gene            complement(10573..11070)
                     /locus_tag="J1Y83_000944"
     CDS             complement(10573..11070)
                     /locus_tag="J1Y83_000944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HBA7629116.1"
                     /translation="MTGSQVIDAEEDRHKLVVEYKDALQPADFYHNFKQLGIRSVQLI
                     PHLEFDDRGDLTAASVTAELWGKFLIALFECWVRADISRISIELFDTTLQKWCGSEKS
                     QPRRDCQACDWHRLCPHAREDKPDNVLCAGYQAFYSYSAPHMRVMRDLIKQHRSPMEL
                     MTMLR"
     gene            complement(11067..11429)
                     /locus_tag="J1Y83_000945"
     CDS             complement(11067..11429)
                     /locus_tag="J1Y83_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF202 domain-containing protein"
                     /protein_id="HBA7629117.1"
                     /translation="MPDSRKARRIADPGLQPERTSLAWFRTMLGYGALMALAIKHSWH
                     QAGMLFWISIGILAIVALILWHYTRNRNLMDVTNSDFSQFHVVRDKFLISLAVLSLAI
                     LFAVTHIHQLIVFIERVA"
     gene            complement(11419..11766)
                     /locus_tag="J1Y83_000946"
     CDS             complement(11419..11766)
                     /locus_tag="J1Y83_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidH family protein"
                     /protein_id="HBA7629118.1"
                     /translation="MKISRLGEAPDYRFSLANERTFLAWIRTALGFLAAGVGLDQLAP
                     DFATPVIRELLALLLCLFSGGLAMYGYLRWLRNEKAMRLKEDLPYTNSLLIISLILMV
                     VAVIVMGLVLYAG"
     gene            complement(11826..13319)
                     /locus_tag="J1Y83_000947"
     CDS             complement(11826..13319)
                     /locus_tag="J1Y83_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HBA7629119.1"
                     /translation="MKRPNFLFIMTDTQATNMVGCYSGKPLNTQNIDSLAAEGIRFNS
                     AYTCSPVCTPARAGLFTGIYANQSGPWTNNVAPGKNISTMGRYFKDAGYHTCYIGKWH
                     LDGHDYFGTGECPPEWDADYWFDGANYLSELTEKEISLWRNGLNSVEDLQANHIDETF
                     TWAHRISNRAVDFLQQPARADEPFLMVISYDEPHHPFTCPVEYLEKYTDFYYELGEKA
                     EDDLANKPEHHRLWAQAMPSPVGDDGLYHHPLYFACNDFVDDQIGRVINALTPEQREN
                     TWVIYTSDHGEMMGAHKLISKGAAMYDDITRIPLIIRSPQGERRQVDTPVSHIDLLPT
                     MMALADIEKPEILPGENILAVKEPRGVLVEFNRYEIEHDSFGGFIPVRCWVTDDFKLV
                     LNLFTSDELYDRRNDPNEMHNLIDDIHFADVRSKMHDALLDYMDKIRDPFRSYQWNLR
                     PWRKDAQPRWMGAFRPRPQDGYSPVVRDYDTGLPTQGVKVEEKKQKF"
     gene            complement(13316..15031)
                     /locus_tag="J1Y83_000948"
     CDS             complement(13316..15031)
                     /locus_tag="J1Y83_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="solute:sodium symporter family transporter"
                     /protein_id="HBA7629120.1"
                     /translation="MNSLQILSFVGFTLLVAVITWWKVRKTDTGSQQGYFLAGRSLKA
                     PVIAASLMLTNLSTEQLVGLSGQAYKSGMSVMGWEVTSAVTLIFLALIFLPRYLKRGI
                     ATIPDFLEERYDKTTRIIIDFCFLIATGVCFLPIVLYSGALALNSLFHVGESLQLSDG
                     AAIWLLVILLGLAGILYAVIGGLRAMAVADSINGIGLVIGGLMVPVFGLIAMGKGSFM
                     QGIEQLTTVHAEKLNSVGGPTDPLPIGAAFTGLILVNTFYWCTNQGIVQRTLASKSLA
                     EGQKGALLTAVLKMLDPLVLVLPGLIAFHLYQDLPKADMAYPTLVNNVLPVPLVGFFG
                     AVLFGAVISTFNGFLNSASTLFSMGIYRRIINQNAEPQQLVTVGRKFGFFIAVVSVMV
                     APWIANAPQGLYSWMKQLNGIYNVPLVTIIIMGFFFPRIPALAAKVAMGIGIISYITI
                     NYLVKFDFHFLYVLACTFCINVVVMLMIGFIKPRATPFTFKDAFAVDMKPWKNVKIAS
                     IGILFAMIGVYAGLAEFGGYGTRWLAMISYFIAAVVIVYLIFDSWRHRHDPAVTFTPD
                     AKDSL"
     gene            15198..16064
                     /locus_tag="J1Y83_000949"
     CDS             15198..16064
                     /locus_tag="J1Y83_000949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418136.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HBA7629121.1"
                     /translation="MNGKLQSSDVKNETPYNIPLLINENVISSGISLISLWHTYADEH
                     YRVIWPRDKKKPLIANSWVAVYTVQGCGKILLKNDEQITLHGNCIIFLKPMDIHSYHC
                     EGLVWEQYWMEFTPTSMMDIPVGQQSVIYNGEVYNQELTEVAELITSPEAIKNNLAVA
                     FLTKIIYQWICLMHTVGKKDPQRRQIEKLIATLHASLQQRWSVADMAATIPCSEAWLR
                     RLFLRYTGKTPKEYYLDARLDLALSLLKQQGNSVGEVADTLNFFDSFHFSKAFKHKFG
                     YAPSAVLKNTDR"
     gene            complement(16154..17815)
                     /locus_tag="J1Y83_000950"
     CDS             complement(16154..17815)
                     /locus_tag="J1Y83_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative transporter"
                     /protein_id="HBA7629122.1"
                     /translation="MSDIALTVSILALVAVVGLFIGNVKFRGVGLGIGGVLFGGIIVG
                     HFVSQAGMTLSSDMLHVIQEFGLILFVYTIGIQVGPGFFASLRVSGLRLNLFAVLIVI
                     IGGLVTAILHKLFDIPLPVVLGIFSGAVTNTPALGAGQQILRDLGTPMAMVDQMGMSY
                     AMAYPFGICGILFTMWMLRVIFRVNVETEAQQHESTRTNGGALIRTINIRVENPNLHN
                     LAIKDVPILNGDKVICSRLKREETLKVPSPETVIQLGDLLHLVGQPADLHNAQLVIGQ
                     EVDTSLSTKGTDLRVARVVVTNENVLGKRIRDLHFKERYDVVISRLNRAGVELVASSD
                     ISLQFGDILNLVGRPSAIDAVANVLGNAQQKLQQVQMLPVFIGIGLGVLLGSIPVFVP
                     GFPAALKLGLAGGPLIMALILGRIGSIGKLYWFMPPSANLALRELGIVLFLSVVGLKS
                     GGDFIHTLVDGEGLSWIGYGALITAVPLITVGILARMLAKMNYLTMCGMLAGSMTDPP
                     ALAFANNLHPTSGAAALSYATVYPLVMFLRIITPQLLAVLFWGIG"
     gene            complement(18012..18440)
                     /gene="ibpB"
                     /locus_tag="J1Y83_000951"
     CDS             complement(18012..18440)
                     /gene="ibpB"
                     /locus_tag="J1Y83_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock chaperone IbpB"
                     /protein_id="HBA7629123.1"
                     /translation="MRNFDLSPLMRQWIGFDKLANALQNAGESQSFPPYNIEKSDDNH
                     YRITLALAGFRQEDLEIQLEGTRLSVKGTPEQPKEEKKWLHQGLMNQPFSLSFTLAEN
                     MEVSGATFVNGLLHIDLIRNEPEPIAAQRIAISERPALNS"
     gene            complement(18552..18965)
                     /gene="ibpA"
                     /locus_tag="J1Y83_000952"
     CDS             complement(18552..18965)
                     /gene="ibpA"
                     /locus_tag="J1Y83_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006687722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock chaperone IbpA"
                     /protein_id="HBA7629124.1"
                     /translation="MRNFDLSPLYRSAIGFDRLFNHLENNQSQSNGGYPPYNVELVDE
                     NHYRIAIAVAGFAESELEITAQDNLLVVKGAHADEQKERTYLYQGIAERNFERKFQLA
                     ENIHVRGANLVNGLLYIDLERVIPEAKKPRRIEIN"
     gene            19271..19603
                     /locus_tag="J1Y83_000953"
     CDS             19271..19603
                     /locus_tag="J1Y83_000953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceK/YidQ family lipoprotein"
                     /protein_id="HBA7629125.1"
                     /translation="MIRNVLLAFMICSGMTLLGGCSSVMSHTGGKEGTYPGTRASATM
                     IGDDETNWGTKSLAILDMPFTAVLDTILLPWDVFRKDSSVRSRVEKSEANAQATNAVI
                     PPARMPDN"
     gene            complement(19605..20852)
                     /locus_tag="J1Y83_000954"
     CDS             complement(19605..20852)
                     /locus_tag="J1Y83_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418144.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3748 domain-containing protein"
                     /protein_id="HBA7629126.1"
                     /translation="MAGTVLYQDRAMKQITFAPRNHLLTNTNTWTPDSQWLVFDVRPS
                     GASFTGETIERVNIHTGEVEVIYRASQGAYVGVVTVHTKSEKYVFIHGPENPDETWYY
                     DFHHRRGVIVESGKVSNLDAMDITAPYTPGALRGGSHVHVFSPNGERVSFTYNDHVMH
                     ELDPALDLRNVGVAAPFGPVNVQKQHPREYSGSHWCVLVSKTTPTPQPGSDEINRAYE
                     EGWVGNHALAFIGDTLSAKGEKVPELFIVELPQDEAGWKAAGDAPLSGTETTLPAPPR
                     GVVQRRLTFTHHRAYPGLVNVPRHWVRCNPQGTQIAFLMRDDNGIVQLWLISPQGGEP
                     RQLTNHKTDIQSAFNWHPSGEWLGFVLDNRIACTHAQSGEVEYLTENHGNPPSADAVV
                     FSPDGQWLAWMEDGQLWITETDR"
     gene            20920..22020
                     /gene="cbrA"
                     /locus_tag="J1Y83_000955"
     CDS             20920..22020
                     /gene="cbrA"
                     /locus_tag="J1Y83_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin M resistance protein"
                     /protein_id="HBA7629127.1"
                     /translation="MEHFDVAIIGLGPAGSALARQLAGKMQVIALDKKHQHGTEGFSK
                     PCGGLLAPDAQRSFIRDGLTLPVDVIANPQIFSVKTVDVAASLTRNYQRSYININRHA
                     FDLWIKSLIPASVEVYHDSLCRKIWREDDKWHVIFRADGWEQHITARYLVGADGANSM
                     VRRHLYPDHQIRKYVAIQQWFAEKHPVPFYSCIFDNAITDCYSWSISKDGYFIFGGAY
                     PMKDGQTRFTTLKEKMSAFQFQFGKAVKSEKCTVLFPSRWQDFVCGKDNAFLIGEAAG
                     FISASSLEGISYALDSAEILRSVLLKQPEKLNTAYRHATRKLRLKLFGKIVKSRCLTA
                     PALRKWIMRSGVAHIPQLKDYPTRFTSPTSRM"
     gene            complement(21981..23318)
                     /locus_tag="J1Y83_000956"
     CDS             complement(21981..23318)
                     /locus_tag="J1Y83_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBA7629128.1"
                     /translation="MVSGFAMPKIWRKLAMDIPVNAAKPGRRRYLTLVMIFITVVICY
                     VDRANLAVASAHIQEEFGITKAQMGYVFSAFAWLYTLCQIPGGWFLDRVGSRVTYFIA
                     IFGWSVATLFQGFATGLMSLIGLRAITGIFEAPAFPTNNRMVTSWFPEHERASAVGFY
                     TSGQFVGLAFLTPLLIWIQEMLSWHWVFIVTGGIGIIWSLIWFKVYQPPRLTKGISKA
                     ELDYIRDGGGLVDGDAPVKKEARQPLTAKDWKLVFHRKLIGVYLGQFAVASTLWFFLT
                     WFPNYLTQEKGITALKAGFMTTVPFLAAFIGVLLSGWVADLLVRKGFSLGFARKTPII
                     CGLLISTCIMGANYTNDPMMIMCLMVLAFFGNGFASITWSLVSSLAPMRLIGLTGGVF
                     NFAGGLGGITVPLVVGYLAQGYGFAPALVYISAVALIGALSYILLVGDVKRVG"
     gene            complement(23393..24541)
                     /gene="dgoD"
                     /locus_tag="J1Y83_000957"
     CDS             complement(23393..24541)
                     /gene="dgoD"
                     /locus_tag="J1Y83_000957"
                     /EC_number="4.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020077623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactonate dehydratase"
                     /protein_id="HBA7629129.1"
                     /translation="MKITKITTYRLPPRWMFLKIETDEGVVGWGEPVIEGRARTVEAA
                     VHELSDYLIGQDPSRINDLWQVMYRAGFYRGGPILMSAIAGIDQALWDIKGKVLNAPV
                     WQLMGGLVRDKIKAYSWVGGDRPADVIDGIKTLREIGFDTFKLNGCEELGLIDNSRAV
                     DAAVNTVAQIREAFGNQIEFGLDFHGRVSAPMAKVLIKELEPYRPLFIEEPVLAEQAE
                     YYPKLAAQTHIPLAAGERMFSRFDFKRVLEAGGISILQPDLSHAGGITECYKIAGMAE
                     AYDVTLAPHCPLGPIALAACLHIDFVSYNAVLQEQSMGIHYNKGAELLDFVKNKEDFS
                     MVGGFFKPLTKPGLGVEIDEAKVIEFSKNAPDWRNPLWRHEDNSVAEW"
     gene            complement(24538..25155)
                     /locus_tag="J1Y83_000958"
     CDS             complement(24538..25155)
                     /locus_tag="J1Y83_000958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxy-6-phosphogalactonate aldolase"
                     /protein_id="HBA7629130.1"
                     /translation="MQWQTKLPLIAILRGITPDEALAHVGAVIDAGFDAVEIPLNSPQ
                     WEKSIPAIVDAYGDKALIGAGTVLKPEQVDALARMGCQLIVTPNIHSEVIRRAVGYGM
                     TVCPGCATATEAFTALEAGAQALKIFPSSAFGPQYIKALKAVLPPDIAVFAVGGVTPE
                     NLAQWIDAGCAGAGLGSDLYRAGQSVERTAQQAAAFVKAYREAVQ"
     gene            complement(25139..26017)
                     /locus_tag="J1Y83_000959"
     CDS             complement(25139..26017)
                     /locus_tag="J1Y83_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxygalactonokinase"
                     /protein_id="HBA7629131.1"
                     /translation="MTARYIAIDWGSTNLRAWLYQGDHCLDCRQSEAGVTRLNGKSPA
                     AVLAEVTTDWREENTPVVMAGMVGSNVGWKVAPYLSVPARFSSIGEQLTSAGDNIWII
                     PGLCVSHDDNHNVMRGEETQLIGARTLAPSSLYVMPGTHCKWVQADSQQINDFRTVMT
                     GELHHLLLNHSLIGAGLPPQENSADAFAAGLERGLNAPDILPQLFEVRASHVLGTLPR
                     EQVSEFLSGLLIGAEVASMRDYVTHQQVITLVAGTSLTARYQQAFQAMGCDVTAVAGD
                     TAFQAGIRSIAHAVAN"
     gene            complement(26014..26703)
                     /gene="dgoR"
                     /locus_tag="J1Y83_000960"
     CDS             complement(26014..26703)
                     /gene="dgoR"
                     /locus_tag="J1Y83_000960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-galactonate utilization transcriptional
                     regulator DgoR"
                     /protein_id="HBA7629132.1"
                     /translation="MTLNKTDRIVITLGRQIVQGKYVPGSPLPAEAELCEEFATSRNI
                     IREVFRSLMAKRLIEMKRYRGAFVAPRNQWNYLDTDVLQWVLENDYDPRLISAMSEVR
                     NLVEPAIARWAAERATSSDLAQIESALNEMIANNQDREAFNEADIRYHEAVLQSVHNP
                     VLQQLSIAISSLQRAVFERTWMGDEANMPQTLQEHKALFDAIRHQDGDAAEQAALTMI
                     ASSTRRLKEIT"
     gene            26982..27638
                     /locus_tag="J1Y83_000961"
     CDS             26982..27638
                     /locus_tag="J1Y83_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001339864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA7629133.1"
                     /translation="MKLNLKGFFKAASLCSLAFALTGCITWGLVSNTASRPHAEQWRS
                     DTIKGLSLAEDSNGTKGYVFVGESLDYLLTTGGDEVVNMLNDPAIHGERITVSDNAKF
                     ILSSSNKNFSGAITLYYDWNNEEDKALATQYGFICDTRRCTWMLDGLKGSIHQKNKKA
                     DYSNVMVFHQPFTVGFYEYKATDGVPHGLVNALLPVTLTLDIVTSPLQFLILCTTRNC
                     "
     gene            complement(27682..28494)
                     /gene="yidA"
                     /locus_tag="J1Y83_000962"
     CDS             complement(27682..28494)
                     /gene="yidA"
                     /locus_tag="J1Y83_000962"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HBA7629134.1"
                     /translation="MAIKLIAIDMDGTLLLPDHTISPAVKNAIAAARARGVNVVLTTG
                     RPYAGVHNYLKELHMEQPGDYCITYNGALVQKAADGSTVAQTALSYDDYRFLEKLSRE
                     VGSHFHALDRTTLYTANRDISYYTVHESFVATIPLVFCEAEKMDPNTQFLKVMMIDEP
                     AILDQAIARIPQEVKEKYTVLKSAPYFLEILDKRVNKGTGVKSLADVLGIKPEEIMAI
                     GDQENDIAMIEYAGVGVAMDNAIPSVKEVANFVTKSNLEDGVAFAIEKYVLN"
     gene            complement(28609..29016)
                     /locus_tag="J1Y83_000963"
     CDS             complement(28609..29016)
                     /locus_tag="J1Y83_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001311237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidB family protein"
                     /protein_id="HBA7629135.1"
                     /translation="MIIMGLFDDVVGAFLKGDAGKYQAILSWVEEQGGIQVLLEKLQS
                     GGLGAILSTWLSNQQSNQSVSGEQVESALGTNAVFDLGQKLGVDTSTASSLLAEQLPK
                     IIDALSPQGEVSPQANNDLLSAGMELLKGKLFR"
     gene            complement(29247..31661)
                     /gene="gyrB"
                     /locus_tag="J1Y83_000964"
     CDS             complement(29247..31661)
                     /gene="gyrB"
                     /locus_tag="J1Y83_000964"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HBA7629136.1"
                     /translation="MSNSYDSSSIKVLKGLDAVRKRPGMYIGDTDDGTGLHHMVFEVV
                     DNAIDEALAGHCKEIIVTIHADNSVSVQDDGRGIPTGIHPEEGVSAAEVIMTVLHAGG
                     KFDDNSYKVSGGLHGVGVSVVNALSQKLELVIQREGKIHRQIYEHGVPQAPLAVTGET
                     EKTGTMVRFWPSLETFTNVTEFEYEILAKRLRELSFLNSGVSIRLRDKRDGKEDHFHY
                     EGGIKAFVEYLNKNKTPIHPNIFYFSTEKDGIGVEVALQWNDGFQENIYCFTNNIPQR
                     DGGTHLAGFRAAMTRTLNAYMDKEGYSKKAKVSATGDDAREGLIAVVSVKVPDPKFSS
                     QTKDKLVSSEVKSAVEQQMNELLAEYLLENPTDAKIVVGKIIDAARAREAARRAREMT
                     RRKGALDLAGLPGKLADCQERDPALSELYLVEGDSAGGSAKQGRNRKNQAILPLKGKI
                     LNVEKARFDKMLSSQEVATLITALGCGIGRDEYNPDKLRYHSIIIMTDADVDGSHIRT
                     LLLTFFYRQMPEIVERGHVYIAQPPLYKVKKGKQEQYIKDDEAMDQYQISIALDGATL
                     HTNASAPALAGEALEKLVSEYNAMQKMINRMERRYPKAMLKELIYQPTLTEADLSDEQ
                     TVTRWVNALVSELNDKEQHGSQWKFDVHTNAEQNLFEPIVRVRTHGVDTDYPLDHEFI
                     TGGEYRRICTLGEKLRGLLEEDAFIERGERRQPVASFEQALDWLVKESRRGLSIQRYK
                     GLGEMNPEQLWETTMDPESRRMLRVTVKDAIAADQLFTTLMGDAVEPRRAFIEENALK
                     AANIDI"
     gene            complement(31690..32763)
                     /gene="recF"
                     /locus_tag="J1Y83_000965"
     CDS             complement(31690..32763)
                     /gene="recF"
                     /locus_tag="J1Y83_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HBA7629137.1"
                     /translation="MSLTRLLIRDFRNIETADLALSPGFNFLVGANGSGKTSVLEAIY
                     TLGHGRAFRSLQIGRVIRHEQEAFVLHGRLQGEERETAIGLTKDKQGDSKVRIDGTDG
                     HKVAELAHLMPMQLITPEGFTLLNGGPKYRRAFLDWGCFHNEPGFFTAWSNLKRLLKQ
                     RNAALRQVTRYEQLRPWDKELIPLAEQISTWRAEYSAGIAADMADTCKQFLPEFSLTF
                     SFQRGWEKETEYAEVLERNFERDRQLTYTAHGPHKADLRIRADGAPVEDTLSRGQLKL
                     LMCALRLAQGEFLTRESGRRCLYLIDDFASELDDERRGLLASRLKATQSQVFVSAISA
                     EHVIDMSDENSKMFTVEKGKITD"
     gene            complement(32763..33863)
                     /gene="dnaN"
                     /locus_tag="J1Y83_000966"
     CDS             complement(32763..33863)
                     /gene="dnaN"
                     /locus_tag="J1Y83_000966"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006177590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HBA7629138.1"
                     /translation="MKFTVEREHLLKPLQQVSGPLGGRPTLPILGNLLLQVADGTLSL
                     TGTDLEMEMVARVALVQPHEPGATTVPARKFFDICRGLPEGAEIAVQLEGERMLVRSG
                     RSRFSLSTLPAADFPNLDDWQSEVEFTLPQATMKRLIEATQFSMAHQDVRYYLNGMLF
                     ETEGEELRTVATDGHRLAVCSMPIGQSLPSHSVIVPRKGVIELMRMLDGGDNPLRVQI
                     GSNNIRAHVGDFIFTSKLVDGRFPDYRRVLPKNPDKHLEAGCDLLKQAFARAAILSNE
                     KFRGVRLYVSENQLKITANNPEQEEAEEILDVTYSGAEMEIGFNVSYVLDVLNALKCE
                     NVRMMLTDSVSSVQIEDAASQSAAYVVMPMRL"
     gene            complement(33868..35271)
                     /gene="dnaA"
                     /locus_tag="J1Y83_000967"
     CDS             complement(33868..35271)
                     /gene="dnaA"
                     /locus_tag="J1Y83_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HBA7629139.1"
                     /translation="MSLSLWQQCLARLQDELPATEFSMWIRPLQAELSDNTLALYAPN
                     RFVLDWVRDKYLNNINGLLTSFCGADAPQLRFEVGTKPVTQTPQAAVTSNVAAPAQVA
                     QTQPQRAAPSTRSGWDNVPAPAEPTYRSNVNVKHTFDNFVEGKSNQLARAAARQVADN
                     PGGAYNPLFLYGGTGLGKTHLLHAVGNGIMARKPNAKVVYMHSERFVQDMVKALQNNA
                     IEEFKRYYRSVDALLIDDIQFFANKERSQEEFFHTFNALLEGNQQIILTSDRYPKEIN
                     GVEDRLKSRFGWGLTVAIEPPELETRVAILMKKADENDIRLPGEVAFFIAKRLRSNVR
                     ELEGALNRVIANANFTGRAITIDFVREALRDLLALQEKLVTIDNIQKTVAEYYKIKVA
                     DLLSKRRSRSVARPRQMAMALAKELTNHSLPEIGDAFGGRDHTTVLHACRKIEQLREE
                     SHDIKEDFSNLIRTLSS"
     gene            35568..35648
                     /locus_tag="J1Y83_000968"
     CDS             35568..35648
                     /locus_tag="J1Y83_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA7629140.1"
                     /translation="MTIDKNWLNRSNKDPGRSLRFTRQPV"
     gene            35878..36018
                     /gene="rpmH"
                     /locus_tag="J1Y83_000969"
     CDS             35878..36018
                     /gene="rpmH"
                     /locus_tag="J1Y83_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001686914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HBA7629141.1"
                     /translation="MKRTFQPSVLKRNRSHGFRARMATKNGRQVLARRRAKGRARLTV
                     SK"
     gene            36035..36394
                     /gene="rnpA"
                     /locus_tag="J1Y83_000970"
     CDS             36035..36394
                     /gene="rnpA"
                     /locus_tag="J1Y83_000970"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014172301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HBA7629142.1"
                     /translation="MVKLAFPRELRLLTPSQFTFVFQQPQRAGTPQITILGRLNSLGH
                     PRIGLTVAKKNVRRAHERNRIKRLTRESFRLRQHELPAMDFVVVAKKGVADLDNRALS
                     EALEKLWRRHCRLARGS"
     gene            36358..36615
                     /gene="yidD"
                     /locus_tag="J1Y83_000971"
     CDS             36358..36615
                     /gene="yidD"
                     /locus_tag="J1Y83_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HBA7629143.1"
                     /translation="MAPPLSPGSRVLIALIRVYQRLISPLLGPHCRFTPTCSSYGIEA
                     LRRFGVIKGSWLTVKRVLKCHPLHPGGDDPVPPGPFDTREH"
     gene            36618..38264
                     /gene="yidC"
                     /locus_tag="J1Y83_000972"
     CDS             36618..38264
                     /gene="yidC"
                     /locus_tag="J1Y83_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HBA7629144.1"
                     /translation="MDSQRNLLVIALLFVSFMIWQAWEQDKNPQPQAQQTTQTTTTAA
                     GSAADQGVPASGQGKLISVKTDVLDLTINTRGGDVEQALLPAYPKELNSTQPFQLLET
                     SPQFIYQAQSGLTGRDGPDNPANGPRPLYNVEKDAYVLAEGQNELQVPMTYTDAAGNT
                     FTKTFVLKRGDYAVNVNYNVQNAGEKPLEISTFGQLKQSITLPPHLDTGSSNFALHTF
                     RGAAYSTPDEKYEKYKFDTIADNENLNISSKGGWVAMLQQYFATAWIPHNDGTNNFYT
                     ANLGNGIAAIGYKSQPVLVQPGQTGAMNSTLWVGPEIQDKMAAVAPHLDLTVDYGWLW
                     FISQPLFKLLKWIHSFVGNWGFSIIIITFIVRGIMYPLTKAQYTSMAKMRMLQPKIQA
                     MRERLGDDKQRISQEMMALYKAEKVNPLGGCFPLLIQMPIFLALYYMLMGSVELRQAP
                     FALWIHDLSAQDPYYILPILMGVTMFFIQKMSPTTVTDPMQQKIMTFMPVIFTVFFLW
                     FPSGLVLYYIVSNLVTIIQQQLIYRGLEKRGLHSREKKKS"
     gene            38370..39734
                     /gene="mnmE"
                     /locus_tag="J1Y83_000973"
     CDS             38370..39734
                     /gene="mnmE"
                     /locus_tag="J1Y83_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HBA7629145.1"
                     /translation="MSDNDTIVAQATPPGRGGVGILRISGLKAREVAETVLGKLPKPR
                     YADYLPFKDADGSVLDQGIALWFPGPNSFTGEDVLELQGHGGPVILDLLLKRILTIPG
                     LRIARPGEFSERAFLNDKLDLAQAEAIADLIDASSEQAARSALNSLQGAFSARVNHLV
                     EALTHLRIYVEAAIDFPDEEIDFLSDGKIEAQLNNVIADLDAVRAEARQGSLLREGMK
                     VVIAGRPNAGKSSLLNALAGREAAIVTDIAGTTRDVLREHIHIDGMPLHIIDTAGLRE
                     ASDEVERIGIERAWQEIEQADRVLFMVDGTTTDAVDPAEIWPEFIARLPAKLPITVVR
                     NKADITGETLGMSEVNGHALIRLSARTGEGVDVLRNHLKQSMGFDTNMEGGFLARRRH
                     LQALEQAAEHLQQGKAQLLGAWAGELLAEELRLAQQNLSEITGEFTSDDLLGRIFSSF
                     CIGK"
BASE COUNT         9605 a        10862 c        10198 g         9098 t
ORIGIN      
        1 ctgaatacca ggaattgtta gccgtttccc gtggagacgc tgttgccgat tatattattg
       61 ataatgtctc tattctcgac ctgatcaatg gcggagaaat ttccggccca attgtgatta
      121 aaggacgtta cattgccggt gttggcgcag aatacgctga tgctccggct ttgcagcgga
      181 ttgatgcccg cggcgcaacg gcggtgccag ggtttattga tgctcacctg catattgaat
      241 ccagcatgat gacgccggtc acctttgaaa ctgctaccct gccgcgcggc ctgacgaccg
      301 ttatttgcga ccctcatgaa atcgtcaacg tgatgggaga agccggattc gcctggtttg
      361 cccgctgtgc cgaacaggca aggcaaaacc agtacttaca ggtcagctct tgcgtacccg
      421 ccctggaagg ctgcgatgtt aatggtgcca gttttaccct tgaacagatg ctcgcctggc
      481 gggaccatcc gcaggttacc ggccttgcag aaatgatgga ctaccctggc ataattagcg
      541 ggcagaatgc gctgctcgat aaactggacg catttcgcca cctgacactg gacggtcact
      601 gcccgggttt gggtggtaaa gaacttaacg cctatattgc tgcgggtatt gaaaactgcc
      661 acgaaagtta tcagttggaa gaaggacgcc ggaaattgca actcggcatg tcattgatga
      721 tccgtgaagg gtccgcagcc cgcaatctca acgcgctggc accgttgatc aacgaattta
      781 acagcccgca atgcatgctc tgtaccgatg accgtaaccc gtgggagatc gcccatgaag
      841 gacacatcga tgccttaatt cgccgcctga tcgaacaaca caatgtaccg ctgcatgtgg
      901 catatcgcgt agccagctgg tcgacggcgc gccattttgg tctgaatcac ctcggcttac
      961 tggcacccgg caagcaggcc gatatcgtcc tgttgagcga tgcgcgtaag gtcacggtgc
     1021 agcaggtact ggtgaaaggc gagccgatcg atgcacaaac cttacaggcg gaagagtcgg
     1081 cgagactggc acaatccgcc ccgccatatg gcaataccat tgatcgccaa ccagtttccg
     1141 ccagcgactt tgccctgcaa tttacccccg gaaaacgcta tcgcgttatt gaggtaatcc
     1201 ataacgaact gatcacgcac tcccgctcca gcgtctacag cgaaaacggt tttgatcgcg
     1261 atgatgtgtg ctttattgcc gttcttgagc gttacggaca acgactggct ccggcctgtg
     1321 gtttgctcgg cggctttggc ctgaatgaag gtgcgctggc tgcgacggtc agccatgaca
     1381 gccataatat tgtagtgatc ggtcgtagcg cagaagagat ggcgctggcg gtcaatcagg
     1441 tgattcagga tggcggcggg ctgtgcgtgg tccgtaacgg ccaggtacaa agtcatctac
     1501 cgttgcccat tgccgggcta atgagcaccg acacggcgca gtcactggcg gagcaaattg
     1561 acaccttgaa agccgccgcc cgtgaatgcg gtccgttacc ggatgagccg tttattcaga
     1621 tggcgtttct ttccctgcca gtgatccccg cgctgaaact aaccagtcag gggctgtttg
     1681 atggtgagaa gtttgccttc actacgctgg aagtcacgga ataataaaaa aaagcccggc
     1741 gtcatgccgg gcaaaagtca ccagttacgt ttatgccact gtcaactgct gaattttttt
     1801 ctcgcggcgg attttgcgtt cttccatcac cgccactatc gccatcaggc agatacaacc
     1861 aatcgcggcg atatccagcg cggcgaaggt gcctgcccag ccggtaaggc caaataccgg
     1921 cgtcccatcg gcaatcattc ccagacctaa cttggcaaag ctgtcaccaa tcaggtaagc
     1981 gaaggtgcct ttaataccat cggcagcgcc aatcgctttt ttaggtacaa agccaacagc
     2041 agccacacca atcaacaatt gcgggccaaa gaccaggaaa cccaacgcaa agagagaagc
     2101 caggtagata tattggttgc tggcgtgttg atacacaccg agcgtggcga taatcagcgc
     2161 cagcgcgata caggccacca ggccacggcg accgttcgcc aggtcagaga gccagcccca
     2221 cagcagcgta ccgaccagcg caccagcttc aaacagcgta aagccctgaa tcgccaccgc
     2281 tttagagagt ttcagttcct ggaacgcata cacggttgac cactggtcga taccaatacg
     2341 caccacatag aggaaaatgt tggcaaagca cagcagccag atcactttgt ttttcagcac
     2401 atactcaaca aagatctgcc acttggtcat atcggtagat tctgtctctt tatcctcttc
     2461 actgatctcc tcgccaaaca gctcttcagc tttgccgagg ccataagatt ccggggagtc
     2521 gctgccgtaa cgcagaccga taaaaccaac aatcagcgcg ataatcgacg ggaaaataaa
     2581 catgccgatg acatgaccat cgaacaggta atttgcgccg aacagcgcca cacctgctgc
     2641 gcctgcacca ccaaggttgt gagaaatgtt ccagaaaccg aggaatgtcc cgcgtttacg
     2701 acgcggcgtc catttggtga tggtggagta actgcacgaa ccgccggtac tctggaaaaa
     2761 gccgcttaag gcgtagaagg caatcatcag gaacaggcta accgagccgc tgcccatact
     2821 ggcgctgaag cccagcatac aaatggcaga caggatcagc atgaacggca ggaattgttt
     2881 ggtgtttttg ccgtcggcgt agtaagaaac cagcgtttta cccacgccat aagtgatgga
     2941 gaagcccagg ccgatcatcc ccagctgcgt catgctcaac ccgtaggtcg agatcatgtc
     3001 gttctgcgcg atgttaaagt tcttgcgaat caggtacatc gtaaggtagc cgataaagac
     3061 caccaggtag gattgcatga acggtttgaa ccacattttg cgccgcactt cgagcggaag
     3121 gtccagggtc ggcttgcgaa cctggtttaa gaaagccagc atgggttact cctgaaatga
     3181 atacctgcgt gaggcatgca ttgtaaaaat cagcagcaaa tcaggcctga gatagcgtcc
     3241 aggcaaaacc tggaaaattt cttagttttg ccggatatga agtgaaaagg tgagatgcat
     3301 cacgcttctc gcggtgtctg ggcgttcaaa aagggcagta atagcagtgc ggaaatcccg
     3361 gcggcgatgg cgataaccac aaaaaatccg ctccagtgcc aggtatcgag tactttcgcc
     3421 agcggccaac cagcaagcga cgcaccaaga taagcaaaca agccgacaaa tcccgtcgcc
     3481 gcccctgccg cctctttgtg ggaacactct gccgccgcca tgccgattaa catctgcggg
     3541 ccaaagacaa aaaaaccaat ggtgaagaag caggttgcct gcatcacgta gctggcaaat
     3601 ggcatcagcc acagcgagcc gactgaaagc aaaattccgg cggcgaaaat caaattcatc
     3661 ggcccgcggt tgccgttaaa cagtttgtcc gaaccccaac ccgctaccag cgcaccaata
     3721 aatccgccca gttcaaacat tgtcactgcc gtattcgccg tgaccagatc gacgcccagc
     3781 gtctcggaca tatacaaatt gccccagtcg ttgatcgccg cccgaaccac atagaccagc
     3841 acatagcaaa acgaaagcag ccagatatac ggattcagca acacatattt ggtgaggatc
     3901 tctttacgcg tcagccctgc cccttcttgt tgttgagcaa tctccagtgc gtcgtgccgc
     3961 cattcaccaa ccgccggtaa acctaacgcc tgcgggcgat cgcgcagccg ccagcagaga
     4021 aaaatcccca cgactatcgc catacaaccc gcaatcatca tcccggcacg ccagccgtaa
     4081 tgcagcgcgc tcgctgccat cacaatggga atgagtgcgc cgccgacgtt atgtgccgtg
     4141 ttccataatg cccaccaacc gccgcgctcg gtacgagaat accaggcggt taacagacgc
     4201 gcacacaccg gtgaacccca gccctggaaa aaggcgttca acacccaaag cacggcaaac
     4261 gcccatagcg acgtcgagaa gccaaacaga atgttgataa tgcccgtggc aataagccct
     4321 atccccataa aataacgggc atttgagcga tcgctgacaa tgccggagac aaacttcgac
     4381 acgccgtagg taatgtaaaa cagggtcgct aacaggccaa tatcgctacg gctgagcacg
     4441 ccgttagcaa ggatttctgg tacggcggcg ttaaaacttt tacgcgtgaa gtaaaacagc
     4501 gcgtaaccca gccagatggt cagcagaata tgccgacgcc agtagcgata gcgggcatca
     4561 atttcatgtt tatcagtcat taatggcgca tcggcaggcg ctttcagaaa cggcaacatc
     4621 atcgaacctt agacataacg ttgaggtaga gaaacgctga cacgcgtgcc gtgcagacag
     4681 gaaatggtca atgtgccacc cagcgccgtt acgcgctcgc gcattccggt aaggccaaaa
     4741 ccgtgttgcc cggaatctgg cggtaaaccg ctgccatcgt cttcaatcac cagcattaac
     4801 cgctcatcat gctgccagcc ttgcagcgtg accgcgctgg catcggcatg tttcacaatg
     4861 ttgttcaacc cttcctggca gacgcgaaac agcgtcacgc gctggttttc gcttaacgct
     4921 gattcatcga ttcgccattc gagatgactg acaatgccgc gcccttccag ctccatttcc
     4981 cgcatcagtg agcggatggc ctgctccagg gtgagatcat ccagctggcg cggacgtaac
     5041 cgccctaaca agcggcgcac cgcgtcgtaa acgcccagcg acagttgttc gatgagctgc
     5101 ccgctctgtt tcacgctggc gttatccgcc gcgagccgct ggacaatgcc cgcttgagtt
     5161 cgaatagcag tgatggtctg accgatatca tcgtgcagct cacgtgccac atcacggcgc
     5221 acgctctctt cggtttccag caaccgttca gcgagatgct gattgcgcgc cagttccttt
     5281 tgcagcgact ggttaagttc acgcaaccgc tggatgcctg cgcccagtaa caaccctgtc
     5341 agactttgca ccagcagcga gagcaataaa tccaccggat gatcgcgcca ggtttgactg
     5401 gcgatcagcg cgatggcgtt catcaatgtc gcaatcagcg ctccttgcca accatagtgc
     5461 caggccagcg cgataatcgg cagcgccaga cagaatggcg taaagcgcga cagttcgtcc
     5521 ggcaatccca actggagcca gaggctgata acaaacagta gcaagtacca gaccagatgc
     5581 cgcccacgcc agttgatcgg ctgggaaacc agtgacgggc cgagcggcag ccaggtgtta
     5641 ttggcgagat agtgccagaa caccagacat atcggggcca gcgtcaggcc gccagttaaa
     5701 gtcagcagca gcgcattcca cgactctttg ccgtgccaaa gccagggcag cgactgcaac
     5761 aacgccgccg ccgttaacgc cgcaccctgt aacagcaagg tgcgccagtc acgttgatgg
     5821 cgatagcgcg agatcagcgc cacgggcaac aacgtcagta aactaccgat catcaataag
     5881 gagaaatggg ttaaaccgac cgcctgcatt agccagtaaa tcagcagcca ctccgcaccc
     5941 agtagcaccg gccagtatcc gcgcgggcat tgcagcatta gccctagccg caggccaaac
     6001 ggaaataaca gcaccgccat atcagggcgc tcaaccaggt gcaggctgat actccacagg
     6061 caaaaccatg cggcagagaa gataaaaaag caggcaataa cggtaattaa gcgggagaac
     6121 aacgtcttca tcaccagcca tcaaacatgc ggcgcgccag ttcaacgtcg ttactgacgc
     6181 ccagtttttc catcagattg gcgcgatgga cgtgtaccgt tttcggcgac aagcccagtt
     6241 cggcggcaat ctctttcacc gccattcctt gcgccagttt ttctgccacc tggcgttcgc
     6301 gtttggttag cgggtcctgg cgaccggatg ccagtttaat ggcaatatcc ggcgtcagat
     6361 aacagccgcc agtggcaacc gtatgcaccg cagcaatgag ttcatccggg ctacagcgtt
     6421 tggagagaaa gccgcgcgcc ccggcgttaa gcgcctgctc aaccagcgcc ggactgtcgt
     6481 gaacggagag cataattgtc gccatgcctt tcggcagctg gcttagaagc tccagaccgg
     6541 agatatcagg catggagata tcgcaaatac acacctgcac accgcgcccc ggcagccccg
     6601 ccagcgcctc gcgccccgaa ccaaactcgg caactacctg caaatcaggt tccagcccca
     6661 gcagctgcgc aaagccggag cggacgataa ggtgatcgtc tataagggca acggtgatca
     6721 tggtcttgtc ctggcgggta ataaaacgcg cttaccttaa cgataagcgc gatgttgttc
     6781 aagccttgag cggttactga aaaaacaccg caatcttgtt aaacatggtc ggatcggact
     6841 gattacgctg cactttcacc acatcttcca gcttgtcgat ttggcttatc atctgctcca
     6901 gacgctggtc gtcattgacc agtagccaga tgtggctttt gtcgctgtcc tgaatcggca
     6961 ggcagagaat gccttcaacg ttaaaagcgc ggcgggcaaa aaggccacaa acgtgggtca
     7021 ttacacccgg atggttgcga acggtgagtt ccagaatcac gttatcatga gttgtgtttt
     7081 gcatggctta ttcccccacc atttcagtat tcgccgcacc tggcggcacc atcgggtaca
     7141 ctttttcttc ggcatcaatg cgcacatgga tcagcgccgg gccagggcga ttgatgattt
     7201 cctgcaatgc agcctgcgga tcggcttcgt tattcaaatc acaggtttcg aggccgaatc
     7261 cggcggcaat ctgcataaag ttgattttgc ccggataggt agcggcaaaa acgccctgct
     7321 tgtagaacag actctgttgc tgatgcacca gccccagcgc ttcgttgttc atcaaaatga
     7381 ttttaacatc cagctgattt tcactggcgg tcgccatctc ctgaatattc atcatcaggc
     7441 tgccgtcgcc ggagaagcac aacactttgc gatccgggtt cgccagcgcc gcgccaatcg
     7501 ccgcaggcag gccaaaaccc atcgtgccca gcccgccgga ggtcagccac tggcgtgggc
     7561 gattgagcgg ataggcttgc gcggtccaca tctgatgctg acccacgtcg gtggtgataa
     7621 tcgcattgtc atcgacacag gcggcaacgg cgttgatcag gccgtaatgg cttaatggat
     7681 cgcacgcttt cgggattgga cacgggaatt cacgctgcaa atccgccacc aactggtgcc
     7741 actctgcacg cggttgcgct tccaccagcg ggatcaactg cgccagcacg tcatcaacat
     7801 ccgcctgaat tgccacatgc ggttgcttga ttttgcccag ctctgcgcgg tcgatatcga
     7861 catgaataat tttggcattc ggacagaact gctcggtttt gccaatcgcc cggtcatcaa
     7921 aacgcgcacc gagtacaatc agcagatccg cctcctgcaa aatatagttg gtgctgcgca
     7981 caccgtgcat ccccagcata cccagcgaca gcggatgcgc ttttggcaac atacccagcg
     8041 ccattaaagt catggtggta ggcagttgcg ctttctccgc cagttcacgc actcgcgcag
     8101 gcgcgttgat cacaccacca cccagataaa gcaccgggcg tttggcggcg ttaatcatcg
     8161 ccgccgcgtc acgaatactt tcttcgctaa aggcgggtgc ggcggctttt tctgccatag
     8221 cgggctgtgt ttcaatctca aaaactgccg tttgcacatc cttaggaatg tctatccaca
     8281 ccgggccagg gcggcctgat tgcgcaatgc ggaaggcatc gctcatgacc tgcgggagtt
     8341 cttcgatatg tctgaccaga tagttgtgtt tggtgatggg gatagagatg ccgtaggtgt
     8401 cgacttcctg gaaggcgtcg gtgccgatca tcgaggcggg aacctgacca gtgatgcaaa
     8461 tcagcgggat ggagtccagc ctcgcatcgg caatggcggt caccaggtta gtcgcacccg
     8521 gtccactaca ggccatacag accgccggtt taccgtcggt gcgcgccatt ccctgagcga
     8581 taaaccccgc gccctgttca tggcgagcca gaatatggcg gatttgcgtg ctttggctta
     8641 aggcatcgta aacaggcagg atagaaccgc ccggaatacc cgtcacaatc ttaatgccct
     8701 gcagttccag gaaatgaacg ataaattctg cgccggtaaa gcgcttacgc gtcgatgttg
     8761 tgcccgaact tgccatgctc cagtcctttt cttctgggcc gactttccgg gaggtgctta
     8821 aacgaaaaac cccgcccggt ttgcgccggc ggggttttgg aatcgtgtgt tgttccagtc
     8881 cctacggcgc attgccgacg accaccacca cacgcacgac gaccactgcg gcggatggcg
     8941 cagttggtag tagttttgcg ttgaacatgg aagtagtcat tggggacctt gttggttttg
     9001 tgtttaacaa tatttataca agcacagctt tacaggggag acaatggaaa atttttcttc
     9061 cggataaaaa tacgtggcag atcacatttt gaactgaaac tcaggcaata aagcatcatc
     9121 acctgaaaga cggtcagatt gttgacagag tgcgccttat tcatgtcgaa tacagcgtaa
     9181 acaccttacc ctaccaaccg caccatcact gtttccggca acatttattg ccgcttttgt
     9241 ttacatattc tgccgctaaa caattcccca ttcctggcgt atatctggct aacattcatc
     9301 aatgtgatag attcctctcc cgcatttatg ggaatgcgta gtgacttatt ctaattattt
     9361 ttataaaagc atccgtgata atgaaaaggc atagaaacgt caatttgtta ttgatgttgg
     9421 tattactcgt ggccgtcggt cagatggcgc aaaccattta tattccagct attgccgata
     9481 tggcgcgcga tctcaatgtc cgtgaagggg cggtgcagag cgtaatgggc gcttatctgc
     9541 tgacttacgg tgtctcacag ctgttttatg gcccgatttc cgaccgcgtg ggtcgccgac
     9601 cggtgatcct cgtcggaatg tccattttta tgctggcaac gctggtcgcg gtcacgacct
     9661 ccagtttgat ggtattgatt gccgccagcg cgatgcaggg aatgggcacc ggcgttggcg
     9721 gcgtaatggc gcgtactttg ccgcgcgatt tatatgaacg gacacagttg cgccacgcta
     9781 acagcctgtt aaacatgggg attcttgtca gtccgctgct cgcaccgcta atcggcggtc
     9841 tgctggatac gatgtggaac tggcgcgcct gttatctctt tttgttggtt ctttgtgccg
     9901 gtgtgacctt cagtatggcc cgctggatgc cggaaacgcg tccggtcgac gcaccgcgca
     9961 cgcgcctgct taccagttat aaaacgcttt tcggtaacag cggttttaac tgttatttgc
    10021 tgatgctgat tggcggtctg gccgggattg ccgcctttga agcctgctcc ggcgtgctga
    10081 tgggcgcggt gttagggctg agcagtatga cggtcagtat tttgtttatt ctgccgattc
    10141 cggcggcatt ttttggcgca tggtttgccg gacgtcctaa taaacgcttc tcaacgttga
    10201 tgtggcagtc ggttatctgc tgcctgctgg ctggcttact gatgtggatc cccgactggt
    10261 ttggcgtgat gaatgtctgg acgctgctcg ttcccgccgc gctgttcttt ttcggtgccg
    10321 ggatgctgtt tccactggct accagcggcg cgatggagcc gttccccttc ctggcgggca
    10381 ccgctggcgc gctggtcggc ggtctgcaaa acattggttc cggcgtgctg gcgtcgctct
    10441 ctgcgatgtt gccgcaaacc ggtcagggca gcctgggatt gttgatgacc ttaatgggat
    10501 tgttgatcgt gctgtgctgg ctaccgctgg cgacgcggat gtcgcatcag gggcagcctg
    10561 tttaagctca cgtcaccgca gcatcgtcat cagctccatg ggagaacgat gctgctttat
    10621 cagatcacgc atcacccgca tatgcggcgc ggagtaagaa taaaacgcct gatagcccgc
    10681 acaaagcacg ttgtctggtt tatcttcccg agcgtgcggg cacagccgat gccagtcgca
    10741 cgcctggcag tcacggcgcg gttgcgactt ttcgctgcca caccattttt gtaaggtggt
    10801 gtcaaaaagt tcaatcgaaa tacgactgat atctgcccgc acccaacact cgaacagggc
    10861 aattaaaaat ttcccccaca gctctgctgt caccgaagcg gccgtcagat cgccccggtc
    10921 atcaaattca agatggggaa taagctgtac tgagcggatg cccagctgtt tgaaattgtg
    10981 ataaaaatcg gcaggttgca gagcatcctt atactctaca accagtttat gcctgtcctc
    11041 ttcagcatca atgacctgac ttcctgtcat gcgactctct caataaatac gataagttga
    11101 tgtatatgcg ttacagcaaa cagtattgcg agagataaca ccgcgaggga gatcaaaaat
    11161 ttgtcacgga ctacgtgaaa ttgggaaaaa tcgctattcg tgacatccat taaattgcga
    11221 ttacgggtgt agtgccagag gatcagcgcc acgatggcga ggatgccaat cgaaatccag
    11281 aataacatgc ccgcctggtg ccagctgtgt ttgatagcca acgccatcaa cgcgccgtag
    11341 cccagcatgg tacgaaacca cgccagtgat gtgcgttccg gctgcagccc tgggtcggca
    11401 atgcgtctgg ctttgcggct atccggcata caacaccagt cccatgacga tcactgccac
    11461 gaccatcaat attaagctga tgattaataa gctgttggta tagggcaggt cctctttcag
    11521 gcgcatcgct ttttcattgc gtaaccagcg caagtagcca tacatcgcta acccgccgga
    11581 aaacagacac aacaacaacg ccagcagttc gcggatgacg ggcgtggcga aatctggcgc
    11641 aagttgatca agcccaaccc ccgccgccag aaatcctagt gcagtacgga tccacgccag
    11701 aaaagtacgc tcattagcca gcgagaagcg gtaatcgggc gcttctccga ggcgggaaat
    11761 tttcattgtt gctccttgta aactgttcct cgccgaaaaa ttaggccagg aacagcaata
    11821 atgcatcaga acttctgttt tttctcttcc accttcaccc cttgtgtcgg taaaccggtg
    11881 tcatagtcgc gtaccactgg agaataacca tcctgtgggc gtggacgaaa cgcccccatc
    11941 cagcgcggtt gtgcatcttt gcgccacgga cgcaggttcc attgataact gcggaacgga
    12001 tcgcgaattt tgtccatgta atccaataag gcgtcatgca ttttgctgcg aacgtcggca
    12061 aaatggatat catcgatcag gttatgcatt tcatttgggt cattacggcg atcgtaaagt
    12121 tcatcactgg tgaagaggtt gagcaccagt ttaaagtcat ccgttaccca gcaacgcacc
    12181 ggaataaaac cgccaaagct gtcatgctca atctcgtagc gattaaattc caccaacacg
    12241 ccacgtggct ctttcacggc aaggatattt tcccccggca gaatctctgg tttttcaata
    12301 tctgccagcg ccatcattgt cggcagtaag tcaatatgac tgactggcgt atcgacctgt
    12361 cgccgctccc cttgcggcga acggatgatc agcggaatgc gggtgatgtc gtcatacatc
    12421 gccgctcctt tactgatcag cttatgtgcg cccatcattt cgccgtgatc ggaggtataa
    12481 ataacccacg tattttcacg ttgctctggc gttaaggcgt tgatgacccg tccgatttgg
    12541 tcatcaacaa agtcattgca ggcaaaatag agcggatggt gataaagccc gtcatcaccg
    12601 actggcgatg gcatcgcctg cgcccataag cggtgatgtt ccggtttgtt cgccaggtca
    12661 tcctctgctt tctcacccag ttcgtagtaa aaatcagtgt atttctctaa atactccacc
    12721 ggacaggtga acgggtgatg cggctcatca tacgaaatca ccatcaggaa aggttcgtcg
    12781 gcgcgcgcgg gctgttgcag aaaatccacc gcccgattgc tgatgcggtg cgcccaggtg
    12841 aaggtttcgt caatatggtt cgcctgtaaa tcctcaacgc tgtttaggcc attgcgccac
    12901 aggctaatct ctttctccgt cagttcacta agatagttcg cgccatcgaa ccagtaatca
    12961 gcgtcccact ccggcggaca ctcgccagtg ccgaaatagt catgtccatc gagatgccat
    13021 ttgccgatgt agcaggtgtg atagcccgca tccttaaagt agcgccccat ggtggaaatg
    13081 tttttgcccg gcgcgacgtt gttggtccac gggccggact ggttagcgta gataccggtg
    13141 aatagtccgg cgcgtgcagg tgtacaaacc ggtgaacagg tgtaggcaga attaaagcga
    13201 ataccttctg ccgccagact atcaatattt tgcgtattca gcggtttacc gctatagcaa
    13261 ccgaccatat tggtggcctg ggtatcggtc atgatgaaca gaaaattggg gcgtttcata
    13321 ggctatcctt cgcatcggga gtaaaggtga cggctgggtc gtgacgatgc cgccagctgt
    13381 caaaaatcag gtagacaatc actacggcgg cgatgaaata actgatcatc gctaaccagc
    13441 gcgtaccgta gccaccgaat tcagccagcc cggcatagac gccaatcatc gcgaacagga
    13501 taccaattga cgcaatcttg acgtttttcc acggtttcat gtccaccgca aacgcatctt
    13561 tgaaggtgaa cggcgtggcg cgcggtttga taaaaccgat catcagcatc acgaccacgt
    13621 tgatgcagaa cgtacaggcc agcacataga ggaaatggaa gtcgaacttc accagatagt
    13681 tgatggtgat gtagctgatt atgccaatcc ccatcgccac ttttgccgcc agcgccggaa
    13741 tgcgcgggaa gaaaaagccc ataatgatga tggtaaccag cggcacgttg taaataccgt
    13801 tgagctgttt catccagcta tacagcccct gcggcgcgtt ggcgatccac ggcgcgacca
    13861 tcaccgaaac gacggcgata aagaaaccaa atttgcgccc gacggtgacc agctgttgcg
    13921 gctcggcatt ctggttaatg atgcgccggt aaatacccat actgaataac gtactggcgc
    13981 tattcagaaa gccgttgaag gtactgatca ccgcaccaaa taacaccgca ccgaagaagc
    14041 ccaccagtgg caccggcaga acgttattga ccagcgtcgg gtaggccatg tcggctttcg
    14101 gtaaatcctg atacagatga aacgcaatca accctggcag caccagtacc agcgggtcga
    14161 gcattttcag caccgccgtt aacagcgccc ccttttgccc ttccgccagg ctttttgacg
    14221 ccagcgtgcg ttgcacgatg ccctgatttg tacaccagta aaaggtgttc accagaatca
    14281 aaccggtaaa tgccgcgcca atcggcaagg gatcggtcgg gccgccgact gagtttaatt
    14341 tctcggcgtg aacggtggtg agttgctcaa tgccctgcat aaagctgccc ttgcccatcg
    14401 cgatcaggcc aaataccggc accatcaacc cgccaataac cagcccaata ccgttaatgg
    14461 agtccgccac tgccattgcg cgcagtccgc cgatcaccgc atacaaaatt cccgccaaac
    14521 caagcaaaat caccagtaac cagatagccg caccatcgga aagttgtagc gactcaccca
    14581 catgaaacag gctgttgagc gccaacgcgc cggagtagag aacaatcggc agaaagcaga
    14641 cgccggtggc aatgaggaag cagaagtcga tgataatacg cgtcgtttta tcataacgtt
    14701 cctccagaaa atcggggatg gtggcaatgc cgcgcttcag atagcgcggt aaaaagatta
    14761 gcgcgaggaa gatcagcgtc actgcagagg tgacttccca gcccatcacc gacatgccgc
    14821 ttttgtaggc ctgcccggaa aggccgacca gttgttccgt ggaaaggttg gttagcatta
    14881 acgaagcggc aataaccggc gcttttagtg aacgtccggc aagaaaatag ccttgttgcg
    14941 atccggtatc tgttttacgg accttccacc aggtgatgac cgctaccagc agcgtaaaac
    15001 cgacaaaact caagatttgt aacgaattca tctcatagcc cttatttatg tttatgtccc
    15061 ggacgcatga atatagggag aggtaaaatg cgtcgggtca ggcagtcaga ttcttaaaat
    15121 acaaacgtcg tatccctgaa cggattgttt tctgttaagt tcaggttgtg agcataattc
    15181 gccggaggga tattaaaatg aatggaaaat tgcaaagttc ggatgtaaaa aacgaaactc
    15241 cgtacaatat tcccttattg attaacgaaa atgtgatctc cagcggaatt tctctgatat
    15301 cgctctggca tacctacgcc gacgagcatt atcgggtgat ctggccgcgg gacaagaaga
    15361 aaccgcttat cgccaactca tgggttgcgg tgtataccgt acaaggatgt gggaaaattc
    15421 ttttaaagaa tgacgaacaa ataactctgc atggcaactg tattatattt ttaaagccaa
    15481 tggatattca ctcttatcac tgtgaaggtt tggtctggga acagtactgg atggaattta
    15541 cccccaccag tatgatggat attcccgtcg gtcagcaaag cgttatttat aatggcgaag
    15601 tttataatca ggaactcacc gaagttgctg agttaattac ttcaccagaa gcaataaaaa
    15661 ataatctggc agtcgctttt ctgacgaaaa ttatttatca gtggatttgt cttatgcaca
    15721 cagtcggtaa aaaagatcca caacggcggc aaattgaaaa attaattgcc actttacatg
    15781 ccagtctaca acaacgctgg agcgtagctg atatggctgc cacgatcccc tgtagcgagg
    15841 cctggttgcg tcgtctgttt ttacgctata ccggcaagac gccgaaagaa tattacctcg
    15901 atgcgcgtct ggatctggcg ctatcgctat taaagcaaca agggaactcg gttggcgaag
    15961 tcgctgatac gctcaacttc ttcgactcct ttcatttcag caaagccttt aagcataaat
    16021 ttggttatgc gccgtcagct gtgctgaaga atacggacag gtaataacca gaatggcgag
    16081 tgtgtaaatg actcgatgat gttcagaaat accaggcgga gtaccattta cggcgactcc
    16141 accgggaaaa gagttaaccg ataccccaga agagcaccgc cagtaattgg ggtgtgataa
    16201 tgcgcaggaa catcactaac ggatagacag tggcgtaaga gagcgccgcc gcaccgctgg
    16261 ttggatgaag attattagca aacgccagcg ccggaggatc ggtcatggaa cctgccagca
    16321 tcccgcacat ggtcaggtaa ttcattttgg ctaacatccg cgccagaatg ccaacagtaa
    16381 tcagcggaac ggcggtgatc agggcaccat aaccaatcca gcttagccct tcgccatcca
    16441 ccagtgtatg gataaaatcg ccgccggatt tcaggccaac caccgagaga aagagcacaa
    16501 tgcctaactc gcgtagcgcc aggttagcgc ttggtggcat aaaccagtac agcttgccga
    16561 tactgccgat acgcccgagg atcaacgcca taatcagcgg accgcccgcc agcccgagtt
    16621 tcaacgccgc cgggaaccca ggcacaaaga cgggaataga acctaacaat acgccaagcc
    16681 cgataccaat aaacactggc agcatctgaa cctgttgcag tttttgctgc gcattcccca
    16741 gcacattggc aacggcatca attgcggacg gacgccccac caggttgagg atatcgccga
    16801 actgcaggct gatatcgcta cttgcaacta attcaacacc agcacggttc aggcgcgaga
    16861 taacaacgtc atagcgttct ttaaagtgca ggtcgcgaat acgtttaccg agcacgtttt
    16921 cattggtgac caccacacgc gcgacgcgca aatcagtgcc tttcgtagac agcgaggtat
    16981 cgacctcctg accaatcacc agttgcgcat tatgtaaatc cgcaggctgc cccaccagat
    17041 gcagcaaatc gcccagttga ataacggttt ctggcgaagg cacttttagg gtttcttctc
    17101 gtttcaatcg tgagcaaata actttatcgc cattgagaat cggcacatct ttaatggcta
    17161 aattatgcaa attaggattc tcaactcgga tgttaatcgt cctgattagc gcaccgccgt
    17221 tggttcgcgt ggactcgtgc tgctgggcct cagtctcaac attgacgcgg aaaataaccc
    17281 gcagcatcca catagtgaac aaaatgccgc aaatcccaaa tggatacgcc atcgcatagc
    17341 tcatccccat ttgatcgacc atcgccatcg gtgtccccag gtcgcgcaag atctgctgcc
    17401 ctgcccccag cgctggcgta ttggtgaccg cgccggaaaa aatccccagc actaccggca
    17461 gtggaatatc aaacagttta tgcaggatgg cggtaaccag accaccgatg atgacgatca
    17521 gaacagcaaa caggttgagg cgtaatccgg agacgcgcaa tgaggcaaag aagcccggcc
    17581 ctacctgaat cccgatggta taaacgaaca ggatcaggcc aaattcctga ataacatgca
    17641 gcatatcgct acttaatgtc atccccgcct gagaaacaaa atggccgacg atgatcccgc
    17701 cgaacagcac gccaccaatg cccaatccaa caccgcgaaa cttgacgttg ccgataaaca
    17761 acccgacgac cgccaccaaa gccagaatac tgaccgttaa tgctatatca ctcatcgtcc
    17821 atttctcttg aaataacatt ttaattgcta cggattatgg ctgaatcggt gaagcagatt
    17881 tgccttacag cgcacattat cacgggaaag aacgtgccga aatatcttaa acaggcgaaa
    17941 tttaacgcat tgctgaagaa aaaagacaaa aaaaagcccc gccagaatgg cggggcaaag
    18001 agaatagcta gttagctatt taacgcggga cgttcgctga tagcgatacg ctgcgctgcg
    18061 atgggttcag gctcattacg aattaaatca atatgcagta aaccgtttac gaaggttgcg
    18121 ccagagactt ccatattttc agccagcgta aagctcaggc taaaaggctg attcataagc
    18181 ccttgatgca gccatttttt ctcttctttt ggctgctctg gcgtgccttt tacgctcagg
    18241 cgcgtacctt ccagttgaat ctctaaatct tcctgacgga aacctgccag cgcaagggta
    18301 atgcggtagt ggttatcgtc gcttttctca atgttgtacg gcgggaagct ctggctttca
    18361 ccggcgtttt gcagtgcgtt ggccagtttg tcaaaaccga tccattgacg catcagtggg
    18421 gataaatcga agttacgcat agtcatttct ccttctaaga agcgagtaat tacctgcaaa
    18481 tccgaagatt cgcatatgct ccctgacggc gagcatggaa atgtcaggcc gcgccaggcg
    18541 gccttaggga attagttgat ttcgatacgg cgcggttttt tcgcttccgg aatcacgcgt
    18601 tcgagatcga tatacagcag accattcacc aggttagcac cacgaacatg aatgttctca
    18661 gctaactgga atttacgttc aaagttgcgt tcagcgatgc cctggtacag ataggtgcgc
    18721 tctttttgtt cgtcggcgtg agcacctttc accaccagca gattatcctg ggcggtaatt
    18781 tccagttcgc tctcagcaaa accagccaca gcgatagcaa tgcggtaatg gttttcgtct
    18841 accagttcaa cgttatacgg agggtagccg ccattactct ggctctggtt gttttctaag
    18901 tggttaaaca aacggtcaaa tccaatagca gaacggtaaa gcggggataa atcaaagtta
    18961 cgcataatca atagctcctg aaatcagcga gaatctaaga ccctccacaa tggacaggtc
    19021 aggtagccag aacacccatc aggcgcgtcc tcatcggcta cgatgtaaaa atgggtctgg
    19081 aaatgaactt ttcaagcctt atcagcggac ttttttttgc agtttatggt ctattgcata
    19141 gactgagggg gcagcacgaa acgttaaagt gcgatagccg ccacagtgcg acgttatgat
    19201 gatgttattt tcagcaaggg atcgctataa ctcatcaggc caacacccac gatcaacaga
    19261 tgaatttatt atgataagaa atgtgttgtt agcgttcatg atatgcagcg gaatgacatt
    19321 actcggagga tgctccagcg tgatgtccca taccgggggg aaagaaggaa cgtatcctgg
    19381 cacccgcgcc agcgcaacca tgattggcga cgatgaaacc aactggggca caaaatcgct
    19441 ggcgatactc gacatgccat ttaccgcagt actggacaca atattgctac cgtgggatgt
    19501 gttccgcaaa gacagctcag tgcgctcgcg cgttgaaaaa agcgaagcca acgcacaagc
    19561 caccaatgcg gttattccac cggccagaat gcctgataac tgatttagcg atcagtttcg
    19621 gtgatccaca actgaccgtc ctccatccac gccaaccatt gaccatccgg cgagaaaacc
    19681 acggcgtccg cagaaggtgg attgccgtgg ttttcggtta aatactcaac ctcgccgctt
    19741 tgcgcatggg tacaagcaat tcgattatct agcacaaagc ctaaccattc acctgacgga
    19801 tgccagttaa atgcagactg aatatccgtt ttgtgattgg ttaactggcg cggctcaccg
    19861 ccctgtggcg agataagcca cagttgcaca atgccattat catcacgcat taaaaacgcg
    19921 atttgcgtac cctgcggatt acagcgcacc cagtggcgcg ggacgttgac taatcccgga
    19981 taagcccgat ggtgggtaaa ggttaaacgt cgctgcacga cgccacgcgg tggtgcaggc
    20041 agggttgttt ccgttccact taacggcgca tcacctgccg ctttccagcc agcttcatct
    20101 tgcggtaact caacgataaa cagctccggc actttctcgc cctttgccga aagtgtgtcg
    20161 ccaataaacg ccagtgcgtg atttcctacc catccttctt cataagcacg attgatttca
    20221 tcactgccag gctgtggcgt gggcgtggtt ttacttacca gcacgcacca gtggctaccg
    20281 ctgtattcac gcggatgctg cttttgtacg ttgaccgggc caaacggtgc agcaacaccg
    20341 acgtttcgca aatccagcgc cggatcgagt tcatgcatta catggtcgtt gtaggtaaag
    20401 ctcacccttt caccgttcgg gctaaagaca tgtacatgac tgccgccgcg cagcgctcct
    20461 ggagtatatg gcgcggtaat atccattgca tcgagattgc tcaccttgcc gctttcaaca
    20521 atcactccgc gccgatgatg aaagtcgtaa taccatgttt catcaggatt ttccgggccg
    20581 tggatgaaga catacttctc tgacttcgta tgaacggtca ccacgccgac gtatgcgccc
    20641 tgtgacgcgc gatagataac ctcgacctcg ccggtatgaa tattcacacg ctcaatggtc
    20701 tcgccggtaa acgacgcccc agaaggccgc acgtcaaaca ccagccactg gctgtcgggc
    20761 gtccaggtat tggtattggt gagcaggtga ttacggggag caaaggttat ctgtttcatg
    20821 gcgcggtcct gatacaaaac ggttcccgcc atcatacttc acaaggactt cactttcaga
    20881 caatagtctt ttgccatcgt gatgaatgtg agtgaaaaga tggaacattt cgacgtggcg
    20941 attattggcc tcggcccggc agggtcggcg ctggcgcggc agttagcagg taaaatgcag
    21001 gtgatcgcgc tggataaaaa gcaccagcat ggtactgaag gtttcagcaa accttgtggc
    21061 ggtctgctgg caccggacgc gcagcgatct tttattcgcg atggactgac gcttcctgtc
    21121 gatgtcatcg ccaacccgca gattttcagc gtcaaaaccg tcgacgtcgc cgcatcgctc
    21181 acgcgtaact accagcgaag ctatatcaat attaatcgcc atgctttcga cttgtggata
    21241 aaatcgctga tccccgccag cgttgaggtt taccacgaca gcctgtgtcg aaaaatctgg
    21301 cgtgaggatg ataaatggca tgtcattttt cgcgcagacg gctgggagca gcatattacc
    21361 gcccgctatc tggtcggtgc cgatggtgcc aactcgatgg tgcggcgaca tctctacccg
    21421 gatcatcaga ttcgtaaata tgtcgccatc cagcagtggt tcgcagagaa acatccggtg
    21481 ccgttctact cctgcatctt tgataatgcg ataactgact gttactcatg gagtatcagc
    21541 aaagacggtt attttatctt tggcggtgcc tatccgatga aagacggtca gacgcgtttc
    21601 acgacgctaa aagagaaaat gagcgccttt cagttccagt ttggtaaggc ggtgaaaagc
    21661 gaaaaatgca cggtgctgtt tccctcacgc tggcaggatt ttgtctgcgg caaggacaac
    21721 gcctttctga ttggcgaagc ggcgggattt atcagcgcca gctcgctgga ggggattagc
    21781 tatgcgctgg atagcgcaga gattctgcgt tcggtattac tgaagcagcc agagaagctc
    21841 aataccgctt acaggcacgc cactcgcaaa ctgcgtttaa aactcttcgg caagatagta
    21901 aaaagccgat gcctgaccgc accggcttta agaaagtgga ttatgcgcag tggagtggcg
    21961 catattccac agttgaaaga ttatccaacg cgcttcacat cccccaccag caggatgtaa
    22021 gagagcgcgc caatcaacgc gacggcggag atataaacca gtgcaggcgc gaaaccgtaa
    22081 ccctgcgcca ggtatcccac caccagcgga acggtgatgc cgcccagacc accggcaaag
    22141 ttaaacacgc cgccggttaa accaatcagg cgcatcggtg ccagagaaga gaccagcgac
    22201 caggttatcg aagcaaaacc gttgccgaag aatgccagca ccatcaggca cataatcatc
    22261 atcggatcgt tggtgtagtt cgcacccata atgcaggtgg agatcagcaa gccgcagata
    22321 atcggcgttt tacgcgcaaa gcccagtgaa aagcccttac gtaccagcag atccgcgacc
    22381 cagccagaga gcaggacgcc aataaacgcc gcgaggaacg gcaccgtggt cataaagcct
    22441 gctttcagcg ctgtgattcc tttttcctgg gttaaatagt tcgggaacca ggttaagaaa
    22501 aaccacagtg tagaagccac cgcaaattgc ccaagataga caccgatcag tttacgatgg
    22561 aacaccagtt tccagtcttt ggctgttaac ggctgacgcg cctctttctt caccggcgca
    22621 tcgccatcca ccagaccgcc gccatcacga atgtaatcca gttcagcttt gctgatgcct
    22681 ttggtcagac gcggtggctg ataaacctta aaccaaatca gcgaccagat aatgccgata
    22741 ccaccggtga caatgaacac ccagtgccag ctcaacatct cctgaatcca gatcagcagc
    22801 ggagtcagaa acgccagacc gacaaactga ccagacgtat aaaaaccaac ggcagaagcg
    22861 cgttcatgtt ccgggaacca gctggtcacc atccggttat tggtcgggaa agcgggcgct
    22921 tcgaaaatac cggttatcgc gcgcagacca attaatgaca ttaatcccgt ggcaaagccc
    22981 tggaataacg tcgccactga ccagccaaat atcgcaataa aataagtcac gcgtgaacct
    23041 acgcgatcta aaaaccaacc gccggggatc tgacatagcg tataaagcca ggcgaaggct
    23101 gaaaatacat agcccatttg cgctttagta ataccaaact cttcctgaat atgggcagaa
    23161 gccacggcca ggttagcgcg gtcaacataa caaatgacca ccgtaataaa gatcatcacc
    23221 agcgtcagat aacgccgacg ccccggcttt gctgcattaa cgggaatatc catagcgagc
    23281 tttctccaga ttttgggcat agcgaagccg ctcaccatgc cctgtaattt acagagggtt
    23341 atatttttgt attgctgttt tagtgcccga tgaggggctt acgtggcagg aattaccact
    23401 ctgctacgct gttatcttca tgacgccaga gcggattacg ccagtccggg gcatttttac
    23461 tgaactcaat aactttagct tcgtcgattt ccacgcctaa gcccggtttc gttaatggtt
    23521 taaagaaacc gccgaccatg ctgaagtctt ctttgttttt cacaaagtcg agtaactccg
    23581 cgcctttgtt gtaatgaatg cccatacttt gttcctgaag gacggcgtta taggaaacaa
    23641 agtcgatatg caggcaagcc gccagtgcaa tcggtccgag cggacagtgc ggcgcaaggg
    23701 tcacgtcata ggcttctgcc attccggcaa ttttgtagca ttcggtaata ccgcctgcgt
    23761 gggagagatc cggttgcaga atcgaaatac cgcctgcctc cagcacacgt ttaaaatcga
    23821 agcgtgagaa catgcgctca cccgccgcca gtggaatatg cgtttgtgcc gccagtttcg
    23881 ggtagtattc ggcctgttcc gccagcaccg gctcttcaat aaacagcggg cgatacggct
    23941 ccagttcttt aatcagcact ttcgccatcg gcgcgctgac gcgaccatgg aaatcaagac
    24001 caaactcaat ctgattgcca aaagcttcac gaatttgtgc cacggtgttg actgccgcat
    24061 ctaccgcgcg ggagttatca attagcccca gttcttcaca accgttcagt ttgaaggtat
    24121 cgaagccgat ttcgcgtagc gttttaatgc cgtcgataac atccgccgga cgatcgccgc
    24181 cgacccaact gtaggcttta attttgtcgc gaaccaggcc gcccatcagt tgccagaccg
    24241 gcgcattcag cactttgcct ttgatatccc ataacgcctg gtcaatcccg gcgatggcgc
    24301 tcatcagaat cggaccgcca cgataaaatc cggcgcgata catcacttgc cataagtcgt
    24361 tgatgcgtga cgggtcctga ccaatcaaat agtcactcag ctcgtggact gccgcttcca
    24421 ccgtgcgagc gcggccttca atcacgggct cgccccaacc gaccacacct tcatcggttt
    24481 caattttcag gaacatccag cggggaggta accgatacgt ggtaattttg gtgattttca
    24541 ttgcactgcc tctcgatacg ccttaacaaa tgctgctgcc tgctgcgcag tccgttctac
    24601 ggactgcccg gcgcgataga gatcgctgcc taagcccgcc cctgcacaac ctgcgtctat
    24661 ccactgcgcc aggttttctg gcgtcacgcc gccaacggca aagactgcga tgtccggtgg
    24721 taataccgct tttaatgctt tgatgtattg cggaccaaaa gccgatgacg gaaatatttt
    24781 cagcgcctgc gcgcccgctt cgagcgcggt aaaggcttcg gtcgccgtcg cacagccggg
    24841 gcagacggtc atgccgtagc ccaccgcacg gcggatcact tcactatgga tattgggcgt
    24901 aacgatgagc tggcagccca tcctggcgag tgcatcgacc tgttcaggtt tcagtaccgt
    24961 acctgcgcca atcaacgcct tatcgccgta tgcatcaacg atggcgggaa tgcttttctc
    25021 ccattgaggg gaattcagcg ggatttcaac cgcgtcgaac ccggcgtcaa tcaccgcgcc
    25081 aacatgtgcc agtgcctcgt cgggcgtaat gccgcgcaaa atggcgatca gcgggagttt
    25141 agtttgccac tgcatgagcg atgctcctta taccagcctg aaatgccgtg tcgcctgcca
    25201 ccgccgtcac gtcgcaaccc atcgcctgaa aggcttgctg gtagcgcgcg gtcagcgatg
    25261 ttccggcgac aagggtgatg acttgttgat gggtcacata gtcgcgcata ctggcgacct
    25321 ctgcgccaat caacaaacca gagagaaatt cgctgacctg ttcgcgggga agtgttccca
    25381 gcacatgcga ggcgcgaact tcaaaaagct gcggcaatat gtcgggagca ttaaggccac
    25441 gctcaagacc agccgcgaaa gcatcggcag agttttcctg cggcggcaaa cctgcgccaa
    25501 tcaatgagtg atttaacagt aaatgatgta attcaccggt catcacggtg cgaaaatcgt
    25561 taatttgctg gctatcggcc tgcacccatt tgcaatgcgt accgggcatg acataaagag
    25621 aggaaggagc gagagtgcgc gcgccgatca attgtgtttc ttcgccgcgc atcacattgt
    25681 ggttatcgtc atgagagacg cataatccgg gaataatcca gatattgtcg ccagcagatg
    25741 ttaattgttc gccaatagac gagaaacggg ctggaacgga taaatacggc gcaactttcc
    25801 agccgacatt gctgccgacc attcctgcca ttaccactgg cgtattctct tcacgccagt
    25861 cggtcgtcac ttctgctaac accgcagccg gagattttcc gttcaggcgc gtgacgcctg
    25921 cttctgattg cctgcagtcc aggcagtggt cgccctgata aagccaggcg cgcagattgg
    25981 tcgatcccca gtcaattgcg atgtagcgag ctgtcatgtg atttccttta accttcgtgt
    26041 cgagctggcg atcatggtaa gcgccgcctg ctctgccgca tcgccgtcct gatgccttat
    26101 cgcatcgaac agcgccttat gttcctggag cgtttgcggc atgttggcct catcgcccat
    26161 ccaggttcgt tcaaaaaccg cccgctgcag cgaactgatc gcaatgctca gttgctgtaa
    26221 caccgggtta tgcaccgact gcagcaccgc ctcgtggtag cgaatatccg cttcgttaaa
    26281 cgcttcgcgg tcctgattgt tagcaatcat ctcgttcagc gccgattcaa tctgcgccag
    26341 atcgctggag gtcgcgcgtt ctgctgccca acgggcaatc gccggttcca ccagatttcg
    26401 cacttcgctc attgcactga taagccgtgg gtcgtagtcg ttttccagca cccattgcag
    26461 tacgtcagtg tcgaggtaat tccactggtt acgcggagcc acaaacgccc cgcgataacg
    26521 tttcatttca atcagccgct tcgccatcag cgaacgaaat acctcacgga tgatgttgcg
    26581 cgaggttgca aactcctcac agagttctgc ctcagccgga agcggcgagc ctggcacgta
    26641 tttgccttga acgatctgtc tacccagcgt aataacgatg cgatcggttt tattgagagt
    26701 catggagagt ccttgtgctc tgtatgttct tctctacttt accccgatcg atgcataacg
    26761 cggcaatttt gtagtaccag cgtgatgacg ttcgcgtttg ctgtgcgcct aatgtagtac
    26821 aaagttatac tgttgtacta caatttagat cacaaaaaga acaatccata agaaaatgtt
    26881 ctacgtcggg caaaaatctg aaaagggaga tcaggcgcta aactgggggg aaagaagagt
    26941 atgctttcaa cggcttagta ctcttgttta aaggattaat catgaagttg aatcttaagg
    27001 gatttttcaa agctgccagt ttgtgttctt tggcatttgc gttgaccgga tgtatcacct
    27061 ggggattggt ttccaatact gcaagccgtc cacatgccga gcaatggcgg tcagacacga
    27121 ttaagggttt atcgctggca gaagatagta acggtacgaa aggctatgtt tttgttggtg
    27181 aatcgttgga ctatctgttg accaccggcg gcgatgaggt agttaacatg ctgaacgatc
    27241 cggcaatcca tggtgaaaga atcaccgttt ctgataacgc gaaatttatt ttaagcagca
    27301 gtaataagaa tttctctggt gctattacgc tctattacga ctggaacaac gaagaagata
    27361 aggcactggc aacacaatat ggttttattt gtgatacccg gcgttgtaca tggatgctgg
    27421 acggtttgaa aggctcaatc caccagaaaa ataaaaaagc ggattactca aacgtgatgg
    27481 ttttccatca accctttacg gtgggatttt atgagtacaa agcaacagat ggcgtcccgc
    27541 acggcctcgt taatgcgctg ctgccagtca ctcttacgct ggatatagta acgtcaccac
    27601 tccagttcct gattttatgc acgaccagaa attgttaatc gctgcgggca aacgcattca
    27661 tgccgtttgc ccgttcactg attaattcag cacatacttc tcaatagcaa atgccacgcc
    27721 atcttcaagg ttagatttgg tgacaaagtt cgccacttct ttcaccgaag gaatagcgtt
    27781 atccatcgcc acaccgacgc ctgcatattc aatcattgcg atgtcgtttt cctgatcgcc
    27841 aatcgccatg atttcttccg gtttaatacc taacacgtcg gccagtgatt tcacacccgt
    27901 acctttgtta acgcgtttat cgaggatttc gaggaagtac ggcgcacttt tcagcacggt
    27961 gtatttttct ttcacttcct gcggaatacg cgcaatggcc tggtcgagga tggcgggttc
    28021 atcaatcatc atcactttca ggaactgggt attggggtcc attttctccg cttcgcagaa
    28081 caccagcgga atggtggcaa cgaaagattc atgcaccgtg tagtagctga tatcacgatt
    28141 ggcggtgtac agcgtggtgc ggtccagcgc gtggaaatga gaaccgactt cgcgagagag
    28201 tttttccagg aaacggtagt cgtcatagct gagagcggtt tgcgccacgg tgctaccatc
    28261 agcggccttc tgtaccagcg cgccgttata agtaatgcag tagtcgcccg gctgttccat
    28321 atgcagctct ttcaggtaat tgtgaacacc tgcataaggg cgacccgtcg ttagcacgac
    28381 attcacgcca cgggcgcgag ctgcggcaat cgcattttta acggcgggtg aaatggtgtg
    28441 atcgggcagc agaagggtgc catccatatc gatagcaatg agtttgatag ccatgagttc
    28501 cccaggtaga ttggttcctg acccatgcta acgcgattcc gctcaaaaat cagtacaaca
    28561 cccgagggaa aagggggatg caacgcgcat tgcgtgctcc ccatttgctt agcggaaaag
    28621 tttccctttc agcagttcca tgcctgcgga aagcagatcg ttattggctt gtggtgacac
    28681 ttcaccttgc ggtgagagcg catcaataat cttcggcaat tgttctgcca gtaaactgga
    28741 agctgtactg gtatccacgc caagtttttg cccgagatcg aacaccgcat ttgtgccgag
    28801 cgccgattcc acttgctcgc cgctaactga ttgattgctc tgttgattac tcagccaggt
    28861 tgagagaatg gcccctaagc cgccactttg cagtttttcc aacagcacct gaatgccgcc
    28921 ctgttcctca acccagctca aaatggcctg atatttcccc gcatcgcctt tcagaaaggc
    28981 accgacaacg tcatcaaaaa gccccatgat aatcacctgt aaagcgttac gtgttgaccc
    29041 aaaaagtata gatctgcggg atgataattg cggattgcag aaataaaaag ggcggagatg
    29101 atctccgccc ttttcttata gcttcttgcc ggatgcggcg tgaacgcctt atccggccta
    29161 caaaaacatg caaattcaat atattgcagg agtctcgtag gcctgataag cgtagcgcat
    29221 caggcacgct cgcattgtta acgccattaa atatcgatat tcgccgcttt cagagcgttc
    29281 tcttcaataa acgcacggcg cggttcaacg gcgtcgccca tcagcgtggt gaacaactgg
    29341 tcggcagcaa tcgcatcttt aacggtaacg cgcaacatac gacggctttc cgggtccatg
    29401 gtagtttccc acagctgttc cgggttcatc tcgcccagac ctttataacg ctggatggag
    29461 aggccgcgac gggactcttt caccagccag tccagcgcct gctcgaagct ggctaccggc
    29521 tgacgacgtt cgccacgttc gataaatgca tcttcttcca gcaagccacg cagtttctca
    29581 cccagcgtgc agatacgacg atattcgccg ccagtgataa attcgtgatc cagcggatag
    29641 tcagtatcca caccgtgggt acgcacgcga acaatcggct cgaacaagtt ttgctcagca
    29701 ttggtgtgga catcaaactt ccactggctg ccgtgctgtt ctttgtcgtt cagttcgctg
    29761 accagcgcgt tcacccagcg ggtaacggtc tgctcatcag agagatcggc ttccgtcagc
    29821 gtcggctgat agataagctc tttcagcatt gccttcggat aacgacgctc catgcggttg
    29881 atcattttct gcatcgcgtt gtactcagac accagtttct ctaacgcttc gccagccagc
    29941 gccggtgcac tggcgttggt gtgcagcgtt gcgccatcca gcgcgataga gatctggtac
    30001 tgatccatcg cttcgtcgtc tttaatgtac tgttcctgct tacctttctt cactttgtac
    30061 agcggcggct gagcgatgta gacgtgacca cgttcaacga tttccggcat ctgacgatag
    30121 aagaaggtca acagcagcgt acgaatgtgc gagccgtcga cgtccgcatc ggtcatgatg
    30181 atgatgctgt gataacgcag tttgtccggg ttgtactcgt cacgaccgat accacaacca
    30241 agcgcggtga taagcgtcgc cacttcctga gaagagagca tcttatcgaa gcgcgctttc
    30301 tcgacgttga ggattttacc cttcagcggc agaatcgcct ggttcttgcg gttacgcccc
    30361 tgcttcgcag agccgcccgc ggagtcccct tccacaaggt acagttcgga aagcgccgga
    30421 tcgcgttcct ggcagtctgc cagtttgccc ggcaggccag ctaaatccag cgcaccttta
    30481 cggcgggtca tttcacgcgc gcgacgcgca gcttcacggg cacgggcagc atcgataatt
    30541 ttgccgacca cgattttcgc gtcggttggg ttttccagca ggtattccgc cagcagttcg
    30601 ttcatctgct gttcaaccgc cgatttcacc tcagaagaaa ccagtttgtc tttggtctgt
    30661 gaggagaatt tcgggtccgg cactttcacg gaaacgaccg caatcaggcc ttcacgcgca
    30721 tcgtcaccgg tggcgctgac tttggctttt ttgctgtagc cttctttgtc catgtaggcg
    30781 ttcagggtgc gggtcatcgc cgcacggaag cctgccaagt gagtaccgcc gtcacgctgc
    30841 ggaatgttgt tggtaaagca gtagatgttt tcctggaagc catcgttcca ctgcaacgcc
    30901 acttcgacgc caataccgtc tttttcggtg gagaagtaga agatattcgg gtggatcggc
    30961 gttttgttct tgttcagata ttcaacgaac gccttgatgc cgccttcata gtggaagtgg
    31021 tcttctttgc catcgcgctt gtcgcgcaga cgaatggaaa cgccggagtt gaggaacgac
    31081 aactcacgca gacgtttcgc cagaatttca tattcgaact cggtcacatt ggtgaaggtt
    31141 tcaaggcttg gccagaaacg caccatggtg ccggtttttt cagtctcgcc ggtaaccgcc
    31201 agcggggcct gcggtacacc gtgttcgtag atctgacggt gaattttacc ctcgcgctgg
    31261 ataaccagct ccagtttttg cgacagggcg tttactaccg aaacaccaac gccgtgcaga
    31321 ccgccggaca ctttatagga gttatcgtca aatttaccgc ctgcgtgcag aacggtcatg
    31381 atcacttccg ccgccgatac gccctcttcc gggtgaatac cggtcggaat gccgcgcccg
    31441 tcatcctgta cggagacaga gttatcggcg tgaatggtga cgataatttc tttacagtga
    31501 cccgcgagcg cttcgtcgat agcgttatct accacctcga ataccatgtg gtgcagaccg
    31561 gtgccgtcat ccgtgtcgcc gatatacata cccgggcgct tacgcaccgc atccagccct
    31621 ttcaggactt tgatactgga ggagtcataa gaattcgaca tcaacgtttc tcgctcattt
    31681 atacttgggt taatccgtta ttttaccctt ttccacggta aacatcttcg aattttcgtc
    31741 cgacatgtct ataacgtgtt cagcactgat cgcgctgaca aagacctgtg attgcgtcgc
    31801 ttttaagcgg ctggcaagca gcccgcgacg ctcatcatca agctcagagg caaaatcatc
    31861 tatcaggtag agacaccgcc gcccgctttc acgggtgagg aactctcctt gcgccagacg
    31921 taaggcgcac atcagcagct taagctgccc acgcgataag gtatcttcca ccggcgcacc
    31981 gtcggcgcga atgcgtaagt ccgctttatg cgggccatgc gcggtgtagg ttagctggcg
    32041 atcgcgttca aaattacgtt ccagcacctc agcatattct gtctctttct cccagccgcg
    32101 ctggaaagag aaagtcagag aaaactcagg gagaaattgc ttacaggtat cggccatatc
    32161 agccgcgata ccggcgctat actccgcgcg ccaggtgctg atttgctccg ccagcgggat
    32221 cagttcttta tcccacggac gtagctgttc gtaacgagtc acctggcgca gcgccgcatt
    32281 gcgctgcttg agcaatcgct tgagattgct ccaggcggtg aaaaatccgg gttcgttgtg
    32341 aaagcatccc cagtcgagga atgctcttct gtatttgggg ccgccgttga gtaaagtaaa
    32401 cccttctggc gttatcagct gcattggcat caggtgcgcc agctccgcga ctttatgccc
    32461 gtctgtaccg tcaatacgga ctttgctgtc gccttgtttg tctttggtta aaccaatcgc
    32521 cgtctcgcgc tcttcgccct gtaatcgccc gtggagaaca aacgcctcct gctcgtggcg
    32581 aatgacgcga ccaatctgca aactgcgaaa cgcccgaccg tgaccgagcg tatagatggc
    32641 ttccagcacg ctggttttgc cactgccgtt ggcacctacc agaaagttaa agccgggaga
    32701 taaggcgaga tccgcggttt caatgttgcg gaaatcgcgg atcaacaagc gggtgaggga
    32761 cattacagtc tcattggcat gacaacataa gccgcgctct ggctggccgc atcttcaatc
    32821 tgcacgctgg aaaccgaatc ggtcagcatc atgcggacgt tttcgcattt cagtgcgttc
    32881 agaacatcca gcacatagct gacgttgaag ccgatttcca tctccgcacc gctataggta
    32941 acgtcgagga tctcttccgc ttcttcctgt tctgggttgt tggcggtgat tttcagctgg
    33001 ttttcgctga catacagacg cacgccgcgg aatttctcgt tagagagaat tgccgcacga
    33061 gcaaacgcct gcttgagcag atcacagcca gcttccagat gcttgtccgg gttcttcggc
    33121 agaacgcgac ggtaatccgg gaagcgacca tccaccagtt tcgaggtgaa gataaagtcg
    33181 ccaacgtggg cgcgaatgtt gttgctgcca atctgcacgc gcagcggatt gtcgccgccg
    33241 tcgagcatac gcatcagttc aatcacgcct ttacgcggca cgatcaccga atggcttggc
    33301 aaagattgac caattggcat tgaacagact gccagacggt ggccgtcggt cgccacggtg
    33361 cgcagttctt caccttcggt ttcaaacagc ataccgttta agtaatagcg aacgtcctga
    33421 tgcgccatag aaaactgggt cgcttcaatc agacgcttca tcgtcgcctg cggcagggta
    33481 aattcgactt cgctctgcca gtcatcgagg ttcgggaaat ctgccgctgg cagggtagac
    33541 agcgaaaaac ggctacgccc ggagcgcacc agcatccgct cgccttccag ctgcacggca
    33601 atttccgcgc cttcaggcag accacggcag atatcaaaga atttgcgcgc cggaacggtc
    33661 gtcgcccctg gttcgtgcgg ctgaaccagc gcaacgcgtg ccaccatttc catctcgaga
    33721 tcagtaccgg tcagcgacaa cgtaccgtca gcaacctgta acagcagatt accgagaatc
    33781 ggtagcgtag gacgaccacc taacggaccg ctcacctgtt gtagcggttt taataaatgc
    33841 tcacgttcta cggtaaattt cataggttta cgatgacaat gttctgatta aattagaaaa
    33901 atcttctttg atatcgtggc tctcttcacg caactgctcg atcttccggc aggcatgaag
    33961 caccgtcgtg tggtcacgac caccaaaggc atcgccaatc tccggcagac tgtggttagt
    34021 cagctctttc gccagcgcca tcgccatctg gcgcggacgg gccaccgagc gggatcgacg
    34081 cttggaaagg agatccgcga ctttgatctt gtagtactcc gccaccgtct tctgaatatt
    34141 gtcgatggtg accagttttt cctgcaatgc cagcaagtcg cgcagcgcct cacgcacgaa
    34201 gtcgatggtg atcgcccgtc cggtaaagtt ggcattggca atgacgcggt tcagcgcgcc
    34261 ttccaactca cgtacgttag atcgtagacg cttggcgata aaaaacgcca cttcgcccgg
    34321 caaacgaatg tcgttttcgt cggccttttt catcaggatc gccacgcgag tttccagctc
    34381 tggcggttcg atcgccacag tcagtcccca gccgaagcgg gatttcaaac gatcctcaac
    34441 gccgttgatc tctttcggat agcgatcgga ggtgagaatg atctgttgat taccttccag
    34501 cagggcgttg aaggtgtgga aaaactcttc ctgagatcgt tctttattag caaaaaactg
    34561 aatatcgtcg atcagcagtg catctacgga acggtagtag cgtttaaact cttcgatcgc
    34621 gttgttttgc agggctttaa ccatgtcctg aacaaagcgc tcggagtgca tataaaccac
    34681 tttggcattc ggcttgcgcg ccataatgcc gttacccacc gcatgcagca ggtgagtttt
    34741 acccaaaccc gtgccgccat aaaggaacaa cgggttataa gcaccgccag ggttatctgc
    34801 cacctggcga gccgccgcgc gcgccagttg gttagattta ccttcaacga agttatcaaa
    34861 cgtgtgtttg acgtttacgt tagaacgata ggtcggttct gccggagccg ggacgttatc
    34921 ccaacccgag cgcgtagaag gcgcagcacg ttgcggctgc gtttgcgcca cctgtgcagg
    34981 ggccgcgacg ttgctcgtca ctgccgcttg tggcgtttgc gtcaccggtt tggtgccgac
    35041 ttcaaaacgc agctgtgggg catccgctcc gcagaaactg gttagcagtc cattgatatt
    35101 attaaggtac ttgtcccgta cccaatcgag gacaaaacgg tttggcgcgt acagggccag
    35161 cgtgttatcg ctcagttccg cctgcaatgg gcgtatccac atactgaatt ctgtggctgg
    35221 taactcatcc tgcaatcggg caagacactg ctgccaaagc gaaagtgaca cggcggactc
    35281 cactcgaaca aaagtcgata atgactaagg ctgaaacatt catgattgtt gacgtacgtc
    35341 gaaaagaccc tgcatgaggg tgacgcacga accgctgtct gcggttatat gcccgatcaa
    35401 gatcctgcaa aacgatcggg accgcggatc atagcctaaa ctgcgcaaga gatcttctgt
    35461 ttctcacaga ttttcccgat ttatccacag gatgatccag aactcgctaa gtgtaaacga
    35521 tcctgccgcg ggccgggcac gatttacgcc gcatattgga aaatttaatg accatagaca
    35581 aaaattggct taatcgatct aataaagatc caggacgatc cttgcgcttt acccgtcagc
    35641 ccgtataatc ctccacccgg cgcgccatgc tggttttcac tggtgtgagg ccgtacgttt
    35701 tccccgcgaa aaggtgcgga aaagcgcggt aaataaggaa agagaattga ctccggagtg
    35761 tacaattatt acaatccggc ctctttaatc acccatggct tcggtgtcca tcgtttcatt
    35821 tttcggcgga tatccaataa agccattgaa tttattcaag tttaggtaga aatcgccatg
    35881 aaacgcactt ttcaaccgtc tgtactgaag cgcaaccgtt ctcacggctt ccgtgctcgt
    35941 atggctacta aaaatggtcg tcaggttctg gcacgtcgtc gtgctaaagg ccgcgctcgt
    36001 ctgaccgttt ctaagtaata aagctaaccc ctgagtggtt aagctcgcat ttcccaggga
    36061 gttacgcttg ttaactccca gtcaattcac attcgtcttc cagcagccac aacgggctgg
    36121 cacgccgcaa attaccattc tcggccgcct gaattcgctg gggcatcccc gtatcggtct
    36181 tacagtcgcc aagaaaaacg ttcgacgcgc ccatgaacgc aatcggatta aacgtctgac
    36241 gcgtgaaagc ttccgtctgc gccaacatga actcccggct atggatttcg tggtggtggc
    36301 gaaaaaaggg gttgccgacc tcgataaccg tgctctctcg gaagcgttgg aaaaattatg
    36361 gcgccgccac tgtcgcctgg ctcgcgggtc ctgatagccc tcattcgggt ctatcaacgc
    36421 ctgattagtc cgctactcgg gccgcattgt cgtttcactc caacctgttc aagctacgga
    36481 attgaggcat tgcgcaggtt tggagtgata aaaggcagtt ggttgacggt gaaacgcgta
    36541 ttaaaatgcc accctttaca ccctggtggt gacgatcccg tcccgcccgg accatttgat
    36601 accagagaac actaacgatg gattcgcaac gcaatctttt agtcatcgct ttgctgttcg
    36661 tgtctttcat gatctggcaa gcctgggagc aggataaaaa cccgcaacct caggcccaac
    36721 agaccacgca gacaacgacc accgcagcgg gtagcgccgc cgaccagggc gtaccggcca
    36781 gtggccaggg gaaactgatc tcggttaaga ccgacgtgct tgatctgacc atcaacaccc
    36841 gtggtggtga tgttgagcaa gccctgctgc ctgcttaccc gaaagagctg aactctaccc
    36901 agccgttcca gctgctggaa acttcaccgc agtttattta tcaggcacag agcggtctga
    36961 ccggtcgtga tggcccggat aacccggcta acggcccgcg tccgctgtat aacgttgaaa
    37021 aagacgctta tgtgctggct gaaggtcaaa acgaactgca ggtgccgatg acgtataccg
    37081 acgcggcagg caacacgttt accaaaacgt ttgtcctgaa acgtggtgat tacgctgtca
    37141 acgtcaacta caacgtgcag aacgctggcg agaaaccgct ggaaatctcc acctttggtc
    37201 agttgaagca atccatcact ctgccaccgc atctcgatac tggaagcagc aacttcgcac
    37261 tgcacacctt ccgtggtgcg gcgtactcca cgcctgacga gaagtacgag aaatacaagt
    37321 tcgataccat tgccgataac gaaaacctga acatctcttc gaaaggtggt tgggtggcaa
    37381 tgctgcaaca gtatttcgcg acggcgtgga tcccgcataa cgacggtacc aacaacttct
    37441 ataccgctaa tctgggtaac ggcatcgccg ctatcggcta taaatctcag ccggtactgg
    37501 ttcagcctgg tcagactggc gcgatgaaca gcaccctgtg ggttggcccg gaaattcagg
    37561 acaaaatggc agctgttgct ccgcacctgg atctgaccgt tgattacggt tggttgtggt
    37621 tcatctctca gccactgttc aaactgctga aatggatcca tagctttgtg ggtaactggg
    37681 gcttctccat tatcatcatc acctttatcg ttcgtggcat catgtacccg ctgaccaaag
    37741 cgcagtacac ctccatggcg aagatgcgta tgctgcagcc gaagattcag gcaatgcgtg
    37801 agcgtctggg cgatgacaaa cagcgcatca gccaggaaat gatggcgctg tacaaagctg
    37861 agaaggttaa cccgctgggc ggctgcttcc cgctgctgat ccagatgcca atcttcctgg
    37921 cgttgtacta catgctgatg ggttccgttg aactacgtca ggcaccgttt gcactgtgga
    37981 tccacgacct gtctgcacag gacccgtact acatcctgcc gatcctgatg ggcgtaacga
    38041 tgttcttcat tcagaagatg tcgccgacca ctgtgaccga cccgatgcag cagaagatca
    38101 tgacctttat gccggtcatc ttcactgtgt tcttcctgtg gttcccgtca ggtctggtgc
    38161 tgtactatat cgtcagcaac ctggtaacca ttattcagca gcagctgatt taccgtggtc
    38221 tggaaaaacg tggcctgcat agccgcgaga agaaaaaatc ctgattcggt gagttttcgc
    38281 taaaataagg gcggtcagtt gaccgccttt tttcttttcg tagggcggat aagcaccaca
    38341 catccgccac acaaagcaac aggaacatca tgagcgataa tgacactatc gtagcccagg
    38401 ccacgcctcc gggacgtggc ggcgttggca tcctgcgcat ctccggcctc aaagcccgtg
    38461 aagttgccga aaccgtgctg ggtaaactgc ctaagccacg ctacgccgat tatcttccgt
    38521 ttaaagacgc cgatggcagc gtgctcgatc aggggattgc gctatggttc cctggcccga
    38581 actcgttcac cggcgaagat gtgctggaac tgcaaggtca tggcggtccg gtgatcctcg
    38641 acctgctgtt aaaacgcatt ctgaccattc ccggcctgcg gattgcccgc cctggtgagt
    38701 tttccgaacg cgcatttctg aacgataaac tcgacttagc ccaggccgag gcgattgcgg
    38761 atcttatcga cgccagctcg gaacaggcgg cccgttcggc gctaaactcg ctgcaaggcg
    38821 cattctccgc acgggtcaac catctggtgg aagccctcac ccacttgcgc atttacgtcg
    38881 aagcggcgat tgatttcccc gatgaagaga tcgatttcct ctccgacggc aaaattgaag
    38941 cccagctcaa taacgtaatt gccgatctcg acgcggtacg cgccgaagct cgtcagggta
    39001 gtttgttgcg cgaagggatg aaagtggtga ttgccggacg tcctaacgcc ggtaaatcga
    39061 gcctgctgaa cgcgctggcg ggccgtgaag cagcaatcgt aaccgatatc gccggaacca
    39121 cgcgtgacgt gctgcgtgag catatccaca ttgacggaat gccgttgcat atcatcgata
    39181 ccgccgggct acgtgaagcc agtgacgaag ttgaacgtat tggtatcgag cgcgcatggc
    39241 aggaaattga acaggcagac cgcgtgctgt ttatggtcga tggcaccacg acagacgccg
    39301 ttgatccggc agagatctgg ccggaattta tcgcccgtct gccagcgaaa ctgccgatca
    39361 ccgtggtgcg caataaagcc gatatcaccg gcgaaacgtt ggggatgagc gaagtgaacg
    39421 gtcacgcgtt aattcgtctt tcggcgcgga ctggcgaagg tgtggatgta ctgcgtaacc
    39481 atctcaaaca gagcatgggc tttgacacca atatggaagg cggcttcctg gcgcgtcgtc
    39541 gtcacctaca ggcgctggaa caggcggcag agcatttgca gcaaggtaaa gcgcaactgt
    39601 taggtgcctg ggcgggtgaa ctgctggcgg aagagttgcg cctggcgcag cagaacttaa
    39661 gcgagatcac cggggaattt acttcagacg acctactggg gcggattttc tccagcttct
    39721 gtatcggtaa gtaaccgcgc ttgcgaagcc gcattctgac tgt
//
LOCUS       DADSYM010000022        38094 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2415
            SAMN08039579-rid11723173.denovo.022, whole genome shotgun sequence.
ACCESSION   DADSYM010000022 DADSYM010000000
VERSION     DADSYM010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039579
            Sequence Read Archive: SRR6362533
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 38094)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38094)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980162.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 03:39:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,484
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 4,347
            CDSs (with protein)               :: 4,347
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 43 of 85
            Pseudo Genes (incomplete)         :: 37 of 85
            Pseudo Genes (internal stop)      :: 19 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38094
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039579-rid11723173.denovo.022"
                     /strain="LGE2415"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8302 N 95.9628 W"
                     /collection_date="2015-08-24"
     gene            complement(133..420)
                     /locus_tag="J1Y83_000974"
     CDS             complement(133..420)
                     /locus_tag="J1Y83_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA7629146.1"
                     /translation="MNEVVNSGVMNIASLVVSVVVLLIGLILWFFINRASSRTNEQIE
                     LLEALLDQQKRQNALLRRLCEANEPEKADKKTVESQKSVEDEDIIRLVAER"
     gene            complement(495..638)
                     /gene="mgrB"
                     /locus_tag="J1Y83_000975"
     CDS             complement(495..638)
                     /gene="mgrB"
                     /locus_tag="J1Y83_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoP/PhoQ regulator MgrB"
                     /protein_id="HBA7629147.1"
                     /translation="MKKFRWVALVVVVLACLLLWAQVFNMMCDQDVQFFSGICAINQF
                     IPW"
     gene            797..1036
                     /locus_tag="J1Y83_000976"
     CDS             797..1036
                     /locus_tag="J1Y83_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA7629148.1"
                     /translation="MRFIIRTVMLIALVWIGLLLSGYGVLIGSKENAAGLGLQCTYLT
                     ARGTSTVQYLHTKSGFLGITDCPLLRKSNIVVDNG"
     gene            complement(1180..1971)
                     /gene="kdgR"
                     /locus_tag="J1Y83_000977"
     CDS             complement(1180..1971)
                     /gene="kdgR"
                     /locus_tag="J1Y83_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012132042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator KdgR"
                     /protein_id="HBA7629149.1"
                     /translation="MANADLDKQPDSVSSVLKVFGILQALGEEREIGITELSQRVMMS
                     KSTVYRFLQTMKTLGYVAQEGESEKYSLTLKLFELGARALQNVDLIRSADIQMREISR
                     LTKETIHLGALDEDSIVYIHKIDSMYNLRMYSRIGRRNPLYSTAIGKVLLAWRDRDEV
                     MQILEGVEYKRSTERTITSTEALLPVLDQVREQGYGEDNEEQEEGLRCIAVPVFDRFG
                     VVIAGLSISFPTLRFSEERLQEYVAMLHTAARKISAQMGYHDYPF"
     gene            2148..3521
                     /locus_tag="J1Y83_000978"
     CDS             2148..3521
                     /locus_tag="J1Y83_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416342.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBA7629150.1"
                     /translation="MPKVQADGLPLPLRYGAILTIVIGISMAVLDGAIANVALPTIAT
                     DLHATPASSIWVVNAYQIAIVISLLSFSFLGDMFGYRRIYKCGLVVFLLSSLFCALSD
                     SLQMLTLARVIQGFGGAALMSVNTALIRLIYPQRFLGRGMGINSFIVAVSSAAGPTIA
                     AAILSIASWKWLFLINVPLGIIALLLAMRFLPPNGSRASKPRFDLPSAVMNALTFGLL
                     ITALSGFAQGQSLTLIGAELVVMVVVGIFFIRRQLSLPVPLLPLDLLRIPLFSLSICT
                     SVCSFCAQMLAMVSLPFYLQTVLGRSEVETGLLLTPWPLATMVMAPLAGYLIERVHAG
                     LLGALGLFIMAAGLFSLVLLPASPTDINIIWPMILCGAGFGLFQSPNNHTIITSAPRE
                     RSGGASGMLGTARLLGQSSGAALVALMLNQFGDNGTHVSLIAAAILALIAACVSGLRI
                     TQPRSRA"
     gene            complement(3567..4448)
                     /gene="htpX"
                     /locus_tag="J1Y83_000979"
     CDS             complement(3567..4448)
                     /gene="htpX"
                     /locus_tag="J1Y83_000979"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014229823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease HtpX"
                     /protein_id="HBA7629151.1"
                     /translation="MMRIALFLLTNLAVMVVFGLVLSLTGIQSSSVQGLMIMALLFGF
                     GGSFVSLLMSKWMALRSVGGEVIEQPRNERERWLVNTVATQARQAGIAMPQVAIYHAP
                     DINAFATGARRDASLVAVSTGLLQNMSPDEAEAVIAHEISHIANGDMVTMTLIQGVVN
                     TFVIFISRILAQLAAGFMGGNRDEGEESNGNPLIYFAVATVLELVFGILASIITMWFS
                     RHREFHADAGSAKLVGREKMIAALQRLKTSYEPQEATSMMAFCINGKSKSLSELFMTH
                     PPLDKRIEALRTGEYLK"
     gene            complement(4640..6688)
                     /gene="prc"
                     /locus_tag="J1Y83_000980"
     CDS             complement(4640..6688)
                     /gene="prc"
                     /locus_tag="J1Y83_000980"
                     /EC_number="3.4.21.102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy terminal-processing peptidase"
                     /protein_id="HBA7629152.1"
                     /translation="MNMFFRLTALAGLLAIAGQTFAVEDITRADQIPVLKEETQHATV
                     SERVTSRFTRSHYRQFDLDQAFSAKIFDRYLNLLDYSHNVLLASDVEQFAKKKTELGD
                     ELRSGKLDVFYDLYNLAQKRRFERYQYALSVLEKPMDFTGNDTYNLDRSKAPWPKNEA
                     ELNALWDSKVKFDELSLKLTGKTDKEIRETLTRRYKFAIRRLAQTNSEDVFSLAMTAF
                     AREIDPHTNYLSPRNTEQFNTEMSLSLEGIGAVLQMDDDYTVINSMVAGGPAAKSKAI
                     SVGDKIVGVGQTGKPMVDVIGWRLDDVVALIKGPKGSKVRLEILPAGKGTKTRTVTLT
                     RERIRLEDRAVKMSVKTVGKEKVGVLDIPGFYVGLTDDVKVQLQKLEKQNVSSVIIDL
                     RSNGGGALTEAVSLSGLFIPSGPIVQVRDNNGKVREDSDTDGQVFYKGPLVVLVDRFS
                     ASASEIFAAAMQDYGRALVVGEPTFGKGTVQQYRSLNRIYDQMLRPEWPALGSVQYTI
                     QKFYRVNGGSTQRKGVTPDIIMPTGNEETETGEKFEDNALPWDSIDAATYVKSGDLTA
                     FEPELLKEHNARIAKDPEFQNIMKDIARFNAMKDKRNIVSLNYAVREKENNEDDATRL
                     ARLNERFKREGKPELKKLDDLPKDYQEPDPYLDETVNIALDLAKLEKARPAEQPAPVK
                     "
     gene            complement(6708..7406)
                     /gene="proQ"
                     /locus_tag="J1Y83_000981"
     CDS             complement(6708..7406)
                     /gene="proQ"
                     /locus_tag="J1Y83_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707288.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone ProQ"
                     /protein_id="HBA7629153.1"
                     /translation="MENQPKLNSSKEVIAFLAERFPHCFSAEGEARPLKIGIFQDLVD
                     RVAGEMNLSKTQLRSALRLYTSSWRYLYGVKPGATRVDLDGNPCGELDEQHVEHARKQ
                     LEEAKARVQAQRAEQQAKKREAAAAAGEKEDAPRRERKPRPTTPRRKEGAERKPRSQK
                     PVEKAPKTVKAPREEQHTPVSDISALTVGQALKVKAGQNAMDATVLEITKDGVRVQLN
                     SGMSLIVRAEHLVF"
     gene            complement(7503..8000)
                     /locus_tag="J1Y83_000982"
     CDS             complement(7503..8000)
                     /locus_tag="J1Y83_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HBA7629154.1"
                     /translation="MNKAEFYADLNRDFNALMAGETSFLATLANTSALLYERLTDVNW
                     AGFYLLEDDTLVLGPFQGKIACVRIPVERGVCGTAVARNQVQRIEDVHAFDGHIACDA
                     ASNSEIVLPLVVKNQIIGVLDIDSTVFGRFTDEDEQGLRQLVAQLEKVLATTDYKKFF
                     ASVAG"
     gene            8130..9413
                     /gene="yebS"
                     /locus_tag="J1Y83_000983"
     CDS             8130..9413
                     /gene="yebS"
                     /locus_tag="J1Y83_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity lipid transport subunit YebS"
                     /protein_id="HBA7629155.1"
                     /translation="MALNTPQITPTKKITVRAIGEELPRGDYQRCPQCDMLFSLPEIN
                     SHQRAYCPRCQAKIRDGRDWSLTRLAAMAFTMLLLMPFAWGEPLLHIWLLGIRIDANI
                     MQGIWQMTKQGDAITGSMVFFCVIGAPLILVTSIAYLWFGNRLGMNLRPVLLMLERLK
                     EWVMLDIYLVGIGVASIKVQDYAHIQAGVGLFSFVALVILTTVTLSHLNVEELWERFY
                     PQRPATRRDEKLRVCLGCHFTGYPDQRGRCPRCHIPLRLRRRHSLQKCWAALLASIVL
                     LLPANLLPISIIYLNGGRQEDTILSGIMSLASSNIAVAGIVFIASILVPFTKVIVMFT
                     LLLSIHFKCQQGLRTRILLLRMVTWIGRWSMLDLFVISLTMSLINRDQILAFTMGPAA
                     FYFGAAVILTILAVEWLDSRLLWDAHESGNARFDD"
     gene            9382..12015
                     /gene="yebT"
                     /locus_tag="J1Y83_000984"
     CDS             9382..12015
                     /gene="yebT"
                     /locus_tag="J1Y83_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416348.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-binding membrane homeostasis protein YebT"
                     /protein_id="HBA7629156.1"
                     /translation="MSQETPASTTEAQIKNKRRISPFWLLPFIALMIAGWLIWDSYQD
                     RGNTVTIDFMSADGIVPGRTPVRYQGVEVGTVQDISLSDDLRKIEVKVSIKSDMKDAL
                     REETQFWLVTPKASLAGVSGLDALVGGNYIGMMPGKGKEQDHFVALDTQPKYRLDNGD
                     LMIHLQAPDLGSLSSGSLVYFRKIPVGKVYDYAINPNKQGVVIDVLIERRFTDLVKKG
                     SRFWNVSGVDANVSISGAKVKLESLAALVNGAIAFDSPEESKPAEAEDTFGLYEDLAH
                     SQRGVIIKLELPDGAGLTADSTPLMYQGLEVGQLTKLDLNPGGKVTGEMTVDPSVVTL
                     LRENTRIELRNPKLSLSDANLSALLTGKTFELVPGDGEPRKEFVVVPGEKALLQEPDV
                     LTLTLTAPESYGIDAGQPLILHGVQVGQVIDRKLTSKGVTFTVAIEPQHRELVKGDSK
                     FVVNSRVDVKVGLDGVEFLGASASEWINGGIRILPGDKGEMKASYPLYANLEKALENS
                     LSDLPTTTVSLSAETLPDVQAGSVVLYRKFEVGEVITVRPRANAFDIDLHIKPEYRNL
                     LTSNSVFWAEGGAKVQLNGSGLTVQASPLSRALKGAISFDNLSGASASQRKGDKRILY
                     ASETAARAVGGQITLHAFDAGKLAVGMPIRYLGIDIGQIQTLDLITARNEVQAKAVLY
                     PEYVQTFARGGTRFSVVTPQISAAGVEHLDTILQPYINVEPGRGNPRRDFELQEATIT
                     DSRYLDGLSIIVEAPEAGSLGIGTPVLFRGLEVGTVTGMTLGTLSDRVMIAMRISKRY
                     QHLVRNNSVFWLASGYSLDFGLTGGVVKTGTFNQFIRGGIAFATPPGTPLAPKAQEGK
                     HFLLQESEPKEWREWGTALPK"
     gene            12095..13534
                     /gene="rsmF"
                     /locus_tag="J1Y83_000985"
     CDS             12095..13534
                     /gene="rsmF"
                     /locus_tag="J1Y83_000985"
                     /EC_number="2.1.1.178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1407)-C(5))-methyltransferase
                     RsmF"
                     /protein_id="HBA7629157.1"
                     /translation="MAQHTVYFPDAFLTQMREAMPSTLSFDDFLAACQRPLRRSIRVN
                     TLKTSVTDFLQLTAPYGWTLTPIPWCEEGFWIERDNEDALPLGSTAEHLSGLFYIQEA
                     SSMLPVAALFADGNAPQRVMDVAAAPGSKTTQIAARMNNEGAILANEFSASRVKVLHA
                     NISRCGISNVALTHFDGRVFGAAVPEMFDAILLDAPCSGEGVVRKDPDALKNWSPESN
                     QEIAATQRELIDSAFHALRPGGTLVYSTCTLNREENEAVCLWLKETYPDAVEFLPLGD
                     LFPGANKARTEEGFLHVFPQIYDCEGFFVARLRKTQAIPALPTPKYKVGNFPFSPVKD
                     REAGQIRQAAASVGLNWDENLRLWQRDKELWLFPVGIEALIGKVRFSRLGIKLAETHN
                     KGYRWQHEAVIALASPDNVNAFELTPQEAEEWYRGRDVYPQAAPVADDVLVTFQHQPI
                     GLAKRIGSRLKNSYPRELVRDGKLFTGNA"
     gene            13652..13888
                     /locus_tag="J1Y83_000986"
     CDS             13652..13888
                     /locus_tag="J1Y83_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1480 family protein"
                     /protein_id="HBA7629158.1"
                     /translation="MKTSVRIGAFEIDDGELHGESPGDRTLTIPCKSDPDLCMQLDAW
                     DAETSIPALLNGEHSVLYRTRYDQQSDAWIMRLA"
     gene            13909..14184
                     /locus_tag="J1Y83_000987"
     CDS             13909..14184
                     /locus_tag="J1Y83_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebW family protein"
                     /protein_id="HBA7629159.1"
                     /translation="MAGYLSGFFPRCKISPKLNGTAPHFGDEMFALVLFVCYLDGGCE
                     DIVVDIYNTEQQCLYSMSDQRIRHGGCFPIEDFIDGFWRPAQEYGDF"
     gene            complement(14185..14841)
                     /gene="pphA"
                     /locus_tag="J1Y83_000988"
     CDS             complement(14185..14841)
                     /gene="pphA"
                     /locus_tag="J1Y83_000988"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416352.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-serine/threonine phosphatase"
                     /protein_id="HBA7629160.1"
                     /translation="MKQPAPVYQRIAGHQWRHIWLSGDIHGCLEQLRRKLWHCRFDPW
                     RDLLISVGDVIDRGPQSLRCLQLLEQHWVRSVRGNHEQMAMDALASRQMSLWLINGGD
                     WFIALADNQQKQAKTALEKCQHLPFILELHSRTGKHVIAHADYPDDVYEWQKDVDLHQ
                     VLWSRSRLGERQKGQGIAGADHFWFGHTPLRHRVDIGNLHYIDTGAVFGGELTLVQLQ
                     "
     gene            complement(15236..15577)
                     /locus_tag="J1Y83_000989"
     CDS             complement(15236..15577)
                     /locus_tag="J1Y83_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebY family protein"
                     /protein_id="HBA7629161.1"
                     /translation="MMKKSILAFLLLTSSAAALAAPQVITVSRFEVGKDKWAFNREEV
                     MLTCRPGNALYVINPSTLVQYPLNDIAQKEVSSGKTKAQPISVIQIDDPNNPGEKMSL
                     APFIERAEKLC"
     gene            complement(15590..16462)
                     /gene="copD"
                     /locus_tag="J1Y83_000990"
     CDS             complement(15590..16462)
                     /gene="copD"
                     /locus_tag="J1Y83_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis membrane protein CopD"
                     /protein_id="HBA7629162.1"
                     /translation="MLAFTWIALRFIHFTSLMLVFGFAMYGAWLAPLTIRRLLTKRFL
                     RLQQHAAVWSLISATAMLAVQGGLMGAGWSDVFSPNIWLAVLQTQFGGVWLWQIVLAL
                     VTLIVALMQPRNMPRLLFMLTTAQFILLAGVGHATLNEGVTAKIHQTNHAIHLICAAA
                     WFGGLLPVLWCMQLIKGRWRHQAIQALMRFSWCGHFAVIGVLASGVLNALLITGFPPT
                     LTTYWGQLLLLKAILVMIMVVIALANRYVLVPRMRQDEDRAAPWFVWMTKLEWAIGAV
                     VLVIISLLATLEPF"
     gene            complement(16466..16840)
                     /gene="yobA"
                     /locus_tag="J1Y83_000991"
     CDS             complement(16466..16840)
                     /gene="yobA"
                     /locus_tag="J1Y83_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopC domain-containing protein YobA"
                     /protein_id="HBA7629163.1"
                     /translation="MASTARSLRYALAILTTSLVTPSVWAHAHLTHQYPAANAQVTAA
                     PQAITLNFSEGVETGFSGAKITGPKNENIKTLPAKRNEQDQKQLIVPLADSLKPGTYT
                     VDWHVVSVDGHKTKGHYTFSVK"
     gene            16979..17209
                     /locus_tag="J1Y83_000992"
     CDS             16979..17209
                     /locus_tag="J1Y83_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit theta"
                     /protein_id="HBA7629164.1"
                     /translation="MLKNLAKLDQTEMDKVNVDLAAAGVAFKERYNMPVIAEAVEREQ
                     PEHLRSWFRERLIAHRLASVNLSRLPYEPKLK"
     gene            17311..17967
                     /locus_tag="J1Y83_000993"
     CDS             17311..17967
                     /locus_tag="J1Y83_000993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HBA7629165.1"
                     /translation="MSFWKVAAAQYEPRKTSLTEQVAHHLEFVRAAARQQCQLLVFPS
                     LSLLGCDYSRRALPAPPDLSLLDPLCYAATTWRMTIIAGLPVEYNDRFIRGIAVFAPW
                     RKTPGIYHQSYGACLGRRSRTITVVDEQPQGMDMDPTCSLFTTGQCLGEPDLLASARR
                     LQFFSHQYSIAVLMANARGNSALWDEHGRLIVRADRGSLLLVGQRSSQGWQGDIIPLR
                     "
     gene            17990..18652
                     /gene="exoX"
                     /locus_tag="J1Y83_000994"
     CDS             17990..18652
                     /gene="exoX"
                     /locus_tag="J1Y83_000994"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease X"
                     /protein_id="HBA7629166.1"
                     /translation="MLRIIDTETCGLQGGIVEIASVDVIDGKIVNPMSHLVRPDRPIS
                     PQAMAIHRITEAMVADKPWIEDVIPHYYGSEWYVAHNASFDRRVLPEMPGEWICTMKL
                     ARRLWPGIKYSNMALYKTRKLNVQTPPGLHHHRALYDCYITAALLIDIMNTSGWTAEQ
                     MADITGRPSLMTTFTFGKYRGKAVSDVAERDPGYLRWLFNNLDSMSPELRLTLKHYLE
                     NT"
     gene            complement(18649..20709)
                     /gene="ptrB"
                     /locus_tag="J1Y83_000995"
     CDS             complement(18649..20709)
                     /gene="ptrB"
                     /locus_tag="J1Y83_000995"
                     /EC_number="3.4.21.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptidase B"
                     /protein_id="HBA7629167.1"
                     /translation="MLPKAARIPHAMTLHGDTRIDNYYWLRDDTRSQPEVLDYLQQEN
                     SYGHRVMASQQALQDRILKEIIDRIPQREVSAPYIKNGYRYRHIYEPGCEYAIYQRQS
                     AFSEEWDEWKTLLDANKRAAHSEFYSMGGMAITPDNTIMALAEDFLSRRQYGIRFRNL
                     ETGNWYPELLDNVEPSFVWANDSWTFYYVRKHPVTLLPYQVWRHAIGTPASQDKLIYE
                     EKDDTYYVSLHKTTSKHYVVIHLASATTSEVRLLDAEMADAEPFVFLPRRKDHEYSLD
                     HYQHRFYLRSNRHGKNFGLYRTRMRDEQQWEELIPPRDNIMLEGFTLFTDWLVVEERQ
                     RGLTSLRQINRKTREVIGIAFDDPAYVTWIAYNPEPETARLRYGYSSMTTPDTLFELD
                     MDTGERRVLKQTEVPGFDAANYRSEHLWIVARDGVEVPVSLVYHRKHFRKGHNPLLVY
                     GYGSYGASIDADFSFSRLSLLDRGFVYAIVHVRGGGELGQQWYEDGKFLKKKNTFNDY
                     LDACDALLKLGYGSPSLCYAMGGSAGGMLMGVAINQRPELFHGVIAQVPFVDVVTTML
                     DESIPLTTGEFEEWGNPQDPQYYEYMKSYSPYDNVTAQAYPHLLVTTGLHDSQVQYWE
                     PAKWVAKLRELKTDDHLLLLCTDMDSGHGGKSGRFKSYEGVAMEYAFLVALAQGTLPA
                     QSAD"
     gene            complement(20918..21577)
                     /locus_tag="J1Y83_000996"
     CDS             complement(20918..21577)
                     /locus_tag="J1Y83_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance TerB family protein"
                     /protein_id="HBA7629168.1"
                     /translation="MANWLNQLQSLLGQSRSSTSSSADQGLGKLLVPGALGGLAGLLV
                     ANKSARKLLTKYGTNALLVGGGAVAGTVLWNKYKDKIRAAHQDEPQFGAQSTPLDERT
                     ERLILALVFAAKSDGHIDAKERAAIDQQLREAGVEEKGRVLIEQAIEQPLDPQRLATG
                     VRNEEEALEIYFLSCAAIDIDHFMERSYLNALGDALKIPQDVREGIERDLEQQKRTLA
                     E"
     gene            complement(21904..22260)
                     /gene="yebF"
                     /locus_tag="J1Y83_000997"
     CDS             complement(21904..22260)
                     /gene="yebF"
                     /locus_tag="J1Y83_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YebF"
                     /protein_id="HBA7629169.1"
                     /translation="MKKRGAFLGLLLVSACASVFAANNETSKSVTFPKCEGLDAAGIA
                     ASVKRDYQQNRVARWADDQKIVGQADPVAWVSLQDIQGKDDKWSVPLTVRGKSADIHY
                     QVSVDCKAGMAEYQRR"
     gene            complement(22327..22617)
                     /gene="yebG"
                     /locus_tag="J1Y83_000998"
     CDS             complement(22327..22617)
                     /gene="yebG"
                     /locus_tag="J1Y83_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein YebG"
                     /protein_id="HBA7629170.1"
                     /translation="MAVEVKYVVIREGEEKMSFTSKKEADAYDKMLDTADLLDTWLTN
                     SPVQMEDEQREALSLWLAEQKDVLSTILKTGKLPSPQVVGAESEEEDASHAA"
     gene            22751..23929
                     /gene="purT"
                     /locus_tag="J1Y83_000999"
     CDS             22751..23929
                     /gene="purT"
                     /locus_tag="J1Y83_000999"
                     /EC_number="2.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent phosphoribosylglycinamide
                     formyltransferase"
                     /protein_id="HBA7629171.1"
                     /translation="MTLLGTALRPAATRVMLLGSGELGKEVAIECQRLGVEVIAVDRY
                     ADAPAMHVAHRSHVINMLDGDALRRVVELEKPHYIVPEIEAIATDMLIQLEEEGLNVV
                     PCARATKLTMNREGIRRLAAEELQLPTSTYRFADSENLFREAVAAIGYPCIVKPVMSS
                     SGKGQTFIRSAEQLAHAWEYAQQGGRAGAGRVIVEGVVKFDFEITLLTVSAVDGVHFC
                     APVGHRQEDGDYRESWQPQQMSPLALERAQEIARKVVLALGGYGLFGVELFVCGDEVI
                     FSEVSPRPHDTGMVTLISQDLSEFALHVRAFLGLPVGGIRQYGPAASAVILPQLTSQN
                     VTFDNVQNAVGADLQIRLFGKPEIDGSRRLGVALATAESVVDAIERAKHAAGQVKVQG
                     "
     gene            complement(23985..24626)
                     /locus_tag="J1Y83_001000"
     CDS             complement(23985..24626)
                     /locus_tag="J1Y83_001000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HBA7629172.1"
                     /translation="MKNWKTSAESILTTGPVVPVIVVKKLEHAVPMAKALVAGGVRVL
                     EVTLRTECAVDAIRAIAKEVPEAIVGAGTVLNPQQLAEVTEAGAQFAISPGLTEPLLK
                     AATEGTIPLIPGISTVSELMLGMDYGLKEFKFFPAEANGGVKALQAIAGPFSQVRFCP
                     TGGISPANYRDYLALKSVLCIGGSWLVPADALEAGDYDRITKLAREAVEGAKL"
     gene            complement(24663..26474)
                     /gene="edd"
                     /locus_tag="J1Y83_001001"
     CDS             complement(24663..26474)
                     /gene="edd"
                     /locus_tag="J1Y83_001001"
                     /EC_number="4.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020244583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphogluconate dehydratase"
                     /protein_id="HBA7629173.1"
                     /translation="MNPQLLRVTNRIIERSRETRSAYLARIEQAKTSTVHRSQLACGN
                     LAHGFAACQPEDKASLKSMLRNNIAIITSYNDMLSAHQPYEHYPEIIRKALHEANAVG
                     QVAGGVPAMCDGVTQGQDGMELSLLSREVIAMSAAVGLSHNMFDGALFLGVCDKIVPG
                     LTMAALSFGHLPAVFVPSGPMASGLPNKEKVRIRQLYAEGKVDRMALLESEAASYHAP
                     GTCTFYGTANTNQMVVEFMGMQLPGSSFVHPDSPLRDALTAAAARQVTRMTGNGNEWM
                     PIGKMIDEKVVVNGIVALLATGGSTNHTMHLVAMARAAGIQINWDDFSDLSDVVPLMA
                     RLYPNGPADINHFQAAGGVPVLVRELLKAGLLHEDVNTVAGFGLSRYTLEPWLNNGEL
                     DWREGAEKSLDNNVIASFEQPFSHHGGTKVLSGNLGRAVMKTSAVPVENQVIEAPAVV
                     FESQHDVMPAFEAGLLDRDCVVVVRHQGPKANGMPELHKLMPPLGVLLDRCFKIALVT
                     DGRLSGASGKVPSAIHVTPEAYDGGLLAKVRDGDIIRVNGQTGELTLLVDEAELAARE
                     PHIPDLSASRVGTGRELFSALREKLSGAEQGATCITF"
     gene            complement(26709..28184)
                     /gene="zwf"
                     /locus_tag="J1Y83_001002"
     CDS             complement(26709..28184)
                     /gene="zwf"
                     /locus_tag="J1Y83_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HBA7629174.1"
                     /translation="MAVTQTAQACDLVIFGAKGDLARRKLLPSLYQLEKAGQLNPDTR
                     IIGVGRADWDKAAYTKVVREALETFMKETIDEGLWDTLSARLDFCNLDVNDTAAFSRL
                     GAMLDQKNRITINYFAMPPSTFGAICKGLGEAKLNAKPARVVMEKPLGTSLATSQEIN
                     DQVGEYFEECQVYRIDHYLGKETVLNLLALRFANSLFVNNWDNRTIDHVEITVAEEVG
                     IEGRWGYFDKAGQMRDMIQNHLLQILCMIAMSPPSDLSADSIRDEKVKVLKSLRRIDR
                     SNVREKTVRGQYTAGFAQGKKVPGYLEEEGANKSSNTETFVAIRVDIDNWRWAGVPFY
                     LRTGKRLPTKCSEVVVYFKTPELNLFKESWQDLPQNKLTIRLQPDEGVDIQVLNKVPG
                     LDHKHNLQITKLDLSYSETFNQTHLADAYERLLLETMRGIQALFVRRDEVEEAWKWVD
                     SITEAWAMDNDAPKPYQAGTWGPVASVAMITRDGRSWNEFE"
     gene            28522..29391
                     /locus_tag="J1Y83_001003"
     CDS             28522..29391
                     /locus_tag="J1Y83_001003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HBA7629175.1"
                     /translation="MNMLEKIQSQLEHLSKSERKVAEVILASPDNAIHSSIAALALEA
                     NVSEPTVNRFCRSMDTRGFPDFKLHLAQSLANGTPYVNRNVNEDDSVESYTGKIFESA
                     MATLDHVRHSLDKSAINRAVDLLTQAKKIAFFGLGSSAAVAHDAMNKFFRFNVPVVYS
                     DDIVLQRMSCMNCSDGDVVVLISHTGRTKNLVELAQLARENDAMVIALTSAGTPLARE
                     ATLAITLDVPEDTDIYMPMVSRLAQLTVIDVLATGFTLRRGTKFRDNLKRVKEALKES
                     RFDKQLLNLSDDR"
     gene            29519..30961
                     /gene="pyk"
                     /locus_tag="J1Y83_001004"
     CDS             29519..30961
                     /gene="pyk"
                     /locus_tag="J1Y83_001004"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HBA7629176.1"
                     /translation="MSRRLRRTKIVTTLGPATDRDNNLEKVIAAGANVVRMNFSHGSP
                     EDHKMRADKVREIAAKLGRHVAILGDLQGPKIRVSTFKEGKVFLNIGDKFLLDANLGK
                     GEGDKEKVGIDYKGLPADVVPGDILLLDDGRVQLKVLEVQGMKVFTEVTVGGPLSNNK
                     GINKLGGGLSAEALTEKDKADIKTAALIGVDYLAVSFPRCGEDLNYARRLARDAGCDA
                     KIVAKVERAEAVCSQEAMDDIILASDVVMVARGDLGVEIGDPELVGIQKALIRRARQL
                     NRAVITATQMMESMITNPMPTRAEVMDVANAVLDGTDAVMLSAETAAGQYPSETVAAM
                     ARVCLGAEKIPSINVSKHRLDVQFDNVEEAIAMSAMYAANHLKGVTAIITMTESGRTA
                     LMTSRISSGLPIFAMSRHERTLNLTALYRGVTPVHFDSANDGVAAASEAVNLLRDKGY
                     LMSGDLVIVTQGDVMSTVGSTNTTRILTVE"
     gene            complement(31093..32064)
                     /gene="lpxM"
                     /locus_tag="J1Y83_001005"
                     /gene_synonym="msbB"
     CDS             complement(31093..32064)
                     /gene="lpxM"
                     /locus_tag="J1Y83_001005"
                     /gene_synonym="msbB"
                     /EC_number="2.3.1.243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416369.1"
                     /note="LpxM is lauroyl-Kdo(2)-lipid IV(A)
                     myristoyltransferase, an enzyme characterized in
                     Escherichia coli and involved in biosynthesis of the form
                     of lipid A found in that species and some closely related
                     species; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lauroyl-Kdo(2)-lipid IV(A) myristoyltransferase"
                     /protein_id="HBA7629177.1"
                     /translation="METKKNNSEYIPEFDKSFRHPRYWGAWLGVAAMAGIALTPPKFR
                     DPILARLGRFAGRLGKSSRRRALINLSLCFPERSEAEREAIVDEMFATAPQAMVMMAE
                     LAIRGPEKIQPRVDWQGLEIIEEMRRNNEKVIFLVPHGWAVDIPAMLMASQGQKMAAM
                     FHNQGNPVFDYVWNTVRRRFGGRLHARNDGIKPFIQSVRQGYWGYYLPDQDHGPEHSE
                     FVDFFATYKATLPAIGRLMKVCRARVVPLFPIYDGKTHRLTIQVRPPMDDLLEADDHT
                     IARRMNEEVEIFVGPRPEQYTWILKLLKTRKPGEIQPYKRKDLYPIK"
     gene            complement(32183..33505)
                     /gene="mepM"
                     /locus_tag="J1Y83_001006"
     CDS             complement(32183..33505)
                     /gene="mepM"
                     /locus_tag="J1Y83_001006"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein DD-endopeptidase MepM"
                     /protein_id="HBA7629178.1"
                     /translation="MQQIARSVALAFNNLPRPHRVMLGSLTVLTLAVAVWRPYVYHRD
                     ATPIVKTIELEQNEIRSLLPEASEPIDQAAQEDEAIPQDELDDKIAGEAGVHEYVVST
                     GDTLSSILNQYGIDMGDITQLAAADKELRNLKIGQQLSWTLTADGELQRLTWEVSRRE
                     TRTYDRTAANGFKMTSEMQQGEWVNNLLKGTVGGSFVASARNAGLTSAEVSAVIKAMQ
                     WQMDFRKLKKGDEFAVLMSREMLDGKREQSQLLGVRLRSEGKDYYAIRAEDGKFYDRN
                     GTGLAKGFLRFPTAKQFRISSNFNPRRTNPVTGRVAPHRGVDFAMPQGTPVLSVGDGE
                     VVVAKRSGAAGYYVAIRHGRSYTTRYMHLRKILVKPGQKVKRGDRIALSGNTGRSTGP
                     HLHYEVWINQQAVNPLTAKLPRTEGLTGSDRREFLAQAKEIVPQLRFD"
     gene            complement(33521..34453)
                     /gene="znuA"
                     /locus_tag="J1Y83_001007"
     CDS             complement(33521..34453)
                     /gene="znuA"
                     /locus_tag="J1Y83_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     ZnuA"
                     /protein_id="HBA7629179.1"
                     /translation="MLHKKTLLFAALSAALWGGATQAADAAVVASLKPVGFIASAIAD
                     GVTETQVLLPDGASEHDYSLRPSDVKRLQNADLVVWVGPEMEAFMQKPVSKLPEAKQV
                     TIAQLENVKPLLMKSIHDDDDDHDHAEKSDEDHHHGDFNMHLWLSPEIARATAVAIHG
                     KLVELMPQSRAKLDANLKDFEAQLASTETQVGNELAPLKGKGYFVFHDAYGYFEKQFG
                     LTPLGHFTVNPEIQPGAQRLHEIRTQLVEQKATCVFAEPQFRPAVVESVARGTSVRMG
                     TLDPLGTNIKLGKTSYSEFLNQLANQYASCLKGD"
     gene            34532..35287
                     /gene="znuC"
                     /locus_tag="J1Y83_001008"
     CDS             34532..35287
                     /gene="znuC"
                     /locus_tag="J1Y83_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter ATP-binding protein ZnuC"
                     /protein_id="HBA7629180.1"
                     /translation="MTSLVSLENVSVSFGQRRVLSDVSLELKPGKILTLLGPNGAGKS
                     TLVRVVLGLVTPDEGVIKRNGKLRIGYVPQKLYLDTTLPLTVNRFLRLRPGTHKEDIL
                     PALKRVQAGHLINAPMQKLSGGETQRVLLARALLNRPQLLVLDEPTQGVDVNGQVALY
                     DLIDQLRRELDCGVLMVSHDLHLVMAKTDEVLCLNHHICCSGTPEVVSLHPEFISMFG
                     PRGAEQLGIYRHHHNHRHDLQGRIVLRRGNDRS"
     gene            35284..36069
                     /gene="znuB"
                     /locus_tag="J1Y83_001009"
     CDS             35284..36069
                     /gene="znuB"
                     /locus_tag="J1Y83_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter permease subunit ZnuB"
                     /protein_id="HBA7629181.1"
                     /translation="MIELLFPGWLAGIMLACAAGPLGSFVVWRRMSYFGDTLAHASLL
                     GVAFGLLLDVNPFYAVIAVTLLLAGGLVWLEKRPQLAIDTLLGIMAHSALSLGLVVVS
                     LMSNIRVDLMAYLFGDLLAVTPEDLISIAIGVVIVVAILFWQWRNLLSMTISPDLAFV
                     DGVKLQRVKLLLMLVTALTIGVAMKFVGALIITSLLIIPAATARRFARTPEQMAGVAV
                     LVGMVAVTGGLTFSAFYDTPAGPSVVLCAALLFILSMMKKQAS"
     gene            complement(36286..37296)
                     /gene="ruvB"
                     /locus_tag="J1Y83_001010"
     CDS             complement(36286..37296)
                     /gene="ruvB"
                     /locus_tag="J1Y83_001010"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017441950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HBA7629182.1"
                     /translation="MIEADRLISAGTTLPEDVADRAIRPKLLEEYVGQPQVRSQMEIF
                     IKAAKLRGDALDHLLIFGPPGLGKTTLANIVANEMGVNLRTTSGPVLEKAGDLAAMLT
                     NLEPHDVLFIDEIHRLSPVVEEVLYPAMEDYQLDIMIGEGPAARSIKIDLPPFTLIGA
                     TTRAGSLTSPLRDRFGIVQRLEFYQVPDLQYIVSRSARFMGLEMSDDGALEVARRARG
                     TPRIANRLLRRVRDFAEVKHDGTISADIAAQALDMLNVDAEGFDYMDRKLLLAVIDKF
                     FGGPVGLDNLAAAIGEERETIEDVLEPYLIQQGFLQRTPRGRMATTRAWNHFGITPPE
                     MP"
     gene            complement(37305..37916)
                     /gene="ruvA"
                     /locus_tag="J1Y83_001011"
     CDS             complement(37305..37916)
                     /gene="ruvA"
                     /locus_tag="J1Y83_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012131997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HBA7629183.1"
                     /translation="MIGRLRGIIIEKQPPLVLIEVGGVGYEVHMPMTCFYELPEAGQE
                     AIVFTHFVVREDAQLLYGFNNKQERTLFKELIKTNGVGPKLALAILSGMSAQQFVNAV
                     EREEVGALVKLPGIGKKTAERLIVEMKDRFKGLHGDLFTPAADLVLTSPASPATDDAE
                     QEAVAALVALGYKPQEASRMVSKIARPDASSETLIREALRAAL"
BASE COUNT         9145 a         9825 c         9354 g         9770 t
ORIGIN      
        1 aattaataat atgataatta tttgaagtca gaccagagtt ttaatttgaa gcagctgcac
       61 catgacagtg cgtataatca gcatgaattg agtaaccctt caacgacggg gttactcaag
      121 attgaggaag gattatcgtt cggcgaccag acgaataata tcttcgtctt caaccgattt
      181 ttgactctca acggtctttt tatctgcttt ctcaggttcg tttgcttcgc acaaacgacg
      241 taacagtgca ttttgacgtt tttgctgatc cagcaacgcc tcaagcagtt caatctgttc
      301 gttagtccgt gaactggcac gattgataaa aaaccacaag atgagcccga taagaagaac
      361 caccaccgat acaaccaaag acgcaatgtt catcacgcct gaatttacaa cttcgttcat
      421 ttcaccacct ctgagtagag gcgctattct accactgctg gagaggaaga aaatctagtg
      481 ctgaaaaaat gatatcacca cgggataaac tggttaatgg cacaaattcc gctgaaaaat
      541 tgtacatcct gatcgcacat catgttgaat acctgcgccc aaagcagcaa gcaagccaac
      601 accacgacaa ccagagcgac ccatcgaaac tttttcactc caatctccgt ttcacctacc
      661 ttatgtcatt aaactagcat gcgttagtta aacagcgcct aactatgtcg atattcgtgc
      721 tttttcggaa tgagtcgctt gcttcagcgc atattgccgc tacgattaag cgaacataaa
      781 aagagaagag gttgtaatgc gattcatcat tcgcacagtt atgctgatag cgctggtatg
      841 gataggatta ttactcagcg gatatggcgt tttgattgga agcaaagaga atgccgcggg
      901 attaggttta caatgtacct atctgaccgc cagaggcacc agcacggtac aatacctgca
      961 cactaaaagt ggttttctgg ggataacaga ttgcccgttg ttacgtaaaa gcaatattgt
     1021 ggtcgataat ggttaaggat aataaataac cagattctca ctcatgagta aaatattctt
     1081 atcgcaagtg cgtttaatat aacgccacct tcaactttat ttttctcatg ctaaataaag
     1141 aacctgtctg tttgatattc agacaggttc ttactctcat cagaacggat agtcgtgata
     1201 acccatttgg gcagaaattt tgcgcgccgc ggtatgcaac attgcgacat attcctgtaa
     1261 acgctcttca gagaaacgta acgtcgggaa ggagatgctc aaaccagcaa tgaccacgcc
     1321 aaagcgatca aataccggaa ccgcaatgca tcgcagccct tcttcctgct cttcattatc
     1381 ttcgccatac ccctgctcgc gcacctggtc cagaacgggt aataacgctt ctgtactggt
     1441 gatggtccgc tcggtactgc gtttatactc cacgccctca agaatttgca tcacttcatc
     1501 gcgatcgcgc catgccagca gtaccttacc aatcgcggtg ctgtacagcg gattacgacg
     1561 cccaatccgt gaatacatgc gcaaattgta cattgagtca attttgtgga tgtaaacaat
     1621 actgtcttcg tccagtgcgc cgaggtggat agtttctttg gtcaggcggg agatctcacg
     1681 catctggata tctgcgctac gaattaaatc gacgttttgc aacgcgcgag cgcccagttc
     1741 gaacaatttc agggtcagcg aatatttctc cgactcccct tcctgcgcca cataacctaa
     1801 agttttcatg gtctgtaaaa agcgataaac ggtgcttttt gacatcatga cgcgctgcga
     1861 caactcggtt atccctattt cgcgctcttc acccagcgcc tgcaaaatgc caaaaacttt
     1921 tagcacggaa gatacagaat caggctgttt atccagatct gcgttagcca tttatcacct
     1981 cattgcgagt gttttataaa aatcagaact gttttttatt ataatttcgc acctgggtgg
     2041 tcgcaatcca tcttttgccg gttagttaca attctgcgac atccaccgtg aatatcagtg
     2101 ctagaatcat acccctgttg attattcacc aaagatataa aattcctatg ccaaaagttc
     2161 aggccgacgg cctgccattg cccctgcgat acggtgcgat attaaccatt gtgattggta
     2221 tttcgatggc tgtccttgac ggcgcaatcg ccaacgtcgc cttgccaaca atcgctacgg
     2281 accttcatgc cacgccagcc agttccatct gggtagtgaa cgcctatcaa atcgccattg
     2341 tcatctccct gctctcgttt tcgtttctgg gcgatatgtt tggctatcga cgtatttata
     2401 aatgcggtct ggtcgttttt ctgttgtctt cactgttctg cgccctttct gattcgctgc
     2461 aaatgctcac ccttgcgcgt gtcatacaag gtttcggcgg tgcagcgttg atgagcgtta
     2521 ataccgcact tatccgcctg atctatccac aacgttttct gggtagaggg atgggcataa
     2581 actcgtttat tgttgccgtc tcttctgctg ccgggccgac aattgctgca gcaatcctct
     2641 ccatcgcatc ctggaaatgg ttatttttaa tcaacgtacc gttgggtatt atcgccctgc
     2701 ttctggcgat gcgttttctg ccacccaatg gttctcgcgc cagtaaaccc cgtttcgacc
     2761 tgcccagcgc cgtgatgaac gcgttaacct tcggcctgct tatcactgcg ttgagtggtt
     2821 tcgctcaggg gcaatcgctg acattgattg gtgcggaact ggtggtaatg gttgtcgttg
     2881 gtattttctt tattcgccgc cagctttctc ttcccgtacc gctgctaccg ctggatttac
     2941 tgcgtatccc gctgttttca ctttctattt gcacatctgt ttgctctttc tgcgcacaaa
     3001 tgctggcaat ggtttccctg cccttttacc tgcaaaccgt gctcgggcgt agtgaagtcg
     3061 aaacaggttt acttctgaca ccgtggccgt tagcaacgat ggtgatggct ccactggcag
     3121 gctatttgat tgaacgcgta catgcaggat tgctgggtgc tttagggtta ttcatcatgg
     3181 ctgcggggct tttttccctg gttctgctgc cagcgtcacc tacggatatc aatattatct
     3241 ggccgatgat cttatgtggt gctggatttg gcttgttcca gtcacccaat aaccacacca
     3301 ttattacctc cgctcctcgc gaacgtagcg gtggagccag tggcatgtta gggacagctc
     3361 gtcttctggg tcagagtagc ggcgcggctc tggtagcgct gatgctaaat cagtttggtg
     3421 ataatggtac gcacgtctcg ctgatagctg cggctattct ggcattgatt gctgcctgtg
     3481 tcagtggttt acgtatcact cagccacgat ccagggcata aaaaaagcgc gtcgatcagg
     3541 acgcgctttt tagtatttac ttcatattac ttcaggtatt cacccgtacg cagagcttca
     3601 atacgtttat ccagcggcgg gtgagtcatg aacaactcac tgagcgattt cgacttcccg
     3661 ttaatacaga aagccatcat gctggttgct tcttgcggtt catagctggt tttcaggcgt
     3721 tgcagcgcgg caatcatttt ctcgcgacca accagttttg ccgaacccgc atcggcgtgg
     3781 aattcacgat gacgcgagaa ccacatggtg ataatgctcg ccagaatacc aaacaccagt
     3841 tccagaaccg ttgcaaccgc aaagtagatc agcgggttgc cgttgctctc ttcaccttca
     3901 tcacggtttc cgcccataaa acccgcggca agctgcgcca gaatacggga aataaagata
     3961 acgaaggtgt tcaccacgcc ctgaatcagc gtcatggtga ccatatcacc attggcgatg
     4021 tggctgattt cgtgagcaat taccgcctcg gcttcatccg ggctcatgtt ctgcagcaaa
     4081 ccggtgctga cagcaaccag agaggcatca cggcgcgcac cggttgcaaa agcattgatg
     4141 tccggcgcat ggtagatagc cacttgcggc atagcgatcc ccgcctgacg agcctgggtt
     4201 gctacagtat tgaccagcca acgttccctt tcgttacgcg gttgctcgat cacttccccg
     4261 ccaacagatc gtaatgccat ccatttggac atcagaagcg aaacgaagga accaccaaaa
     4321 ccgaacagca aggccatgat catcagcccc tgaacgctgc tcgactgtat ccctgtcagg
     4381 ctcagtacca gcccgaaaac gaccattacg gccaggttcg ttagcaggaa gagcgcgatt
     4441 cgcatcataa ttttcttttt acctcagttt aacaaaacgc aatatgcgat acccacatcg
     4501 tatgggttac gcgactattt tcaagtctgg atagtgcgta agtcaccaga aagacacaac
     4561 tttacatttt gtagcatctg atttacggca tcttgccgct gttaaaaaat caggcacaat
     4621 ttcttgtgcc tgattgatat tacttgacgg gagcgggttg ttccgcgggt ctggcttttt
     4681 caagcttcgc cagatcgagt gcgatattca ccgtctcatc cagataagga tctggctcct
     4741 ggtaatcttt cggtagatca tccagtttct tcaactccgg tttaccttcg cgtttaaagc
     4801 gttcgttcaa acgcgccaga cgcgtcgcat catcttcatt attctctttc tcacgcacag
     4861 cgtaattcag agaaacgata ttgcgcttgt ccttcatagc gttgaagcgc gcgatatcct
     4921 tcatgatgtt ctggaactca ggatctttcg cgatacgcgc attatgttcc ttcagcagct
     4981 ccggttcaaa ggccgttaaa tctcctgatt tcacataagt cgcggcatca atgctatccc
     5041 acggcagcgc gttatcttcg aatttctcac ccgtttccgt ttcttcatta cccgtcggca
     5101 tgatgatgtc tggcgttacg cctttacgtt gcgtactgcc gccgttaacg cgatagaatt
     5161 tctggatcgt gtactgcaca gaacccagcg ctggccattc aggacgtaac atctgatcgt
     5221 aaatacggtt caatgaacgg tactgctgaa cggtgccttt accaaatgtc ggttcaccca
     5281 caaccagggc acgaccgtaa tcctgcattg ccgcggcaaa gatttctgaa gccgaagcac
     5341 tgaagcggtc aaccagcacc accagcgggc ctttatagaa gacctgtccg tcggtgtcgc
     5401 tgtcttcacg aaccttgccg ttgttatcgc ggacctgaac aatcgggccg gatggaatga
     5461 acagaccgga gagcgatacg gcttcagtca acgccccacc accattgcta cgcaggtcga
     5521 taatcacact gctgacattc tgtttttcca gtttctgcag ttgcactttg acatcgtctg
     5581 tcaaacccac atagaagccc ggaatatcca gcacgccgac tttctcttta ccgacggtct
     5641 tcaccgacat tttaaccgcg cggtcttcga gacgaatacg ttcacgggtc aacgttaccg
     5701 tacgggtctt ggtcccttta ccagcaggta aaatttccag acgaacctta ctgcccttag
     5761 gccctttaat taaggccacc acatcatcaa gacgccagcc aatcacgtca accatcggct
     5821 tgcctgtttg accaacaccg acaattttgt caccaacgct gatagctttg ctcttcgctg
     5881 ccggacctcc tgccaccatc gaattgataa cggtgtagtc atcatccatt tgcagcactg
     5941 cgccaatacc ttccagcgac aaactcattt cagtgttgaa ctgttcggta ttacgcgggg
     6001 aaagatagtt ggtatgcggg tcgatttcac gcgcaaacgc cgtcattgcc agcgagaaaa
     6061 catcttcgct gttggtttgc gccagacgac gaatggcaaa tttgtagcgg cgagtcaggg
     6121 tttcacgaat ttccttatcc gtttttcctg tcagcttcag gcttaactcg tcgaatttga
     6181 ctttactgtc ccacagcgcg ttcaactcag cctcgttttt cggccagggc gctttgctgc
     6241 ggtcaaggtt ataagtgtcg ttgccggtga aatccatcgg cttttccagt accgacaaag
     6301 cgtactggta acgttcaaaa cggcgctttt gcgccagatt gtagagatcg tagaaaacgt
     6361 cgagtttacc tgaacgcagt tcatcgccta actcagtttt ctttttcgcg aactgttcaa
     6421 catcgcttgc cagcagcaca ttgtggctgt aatcgagcag attcaggtag cggtcaaaga
     6481 ttttggccga aaatgcctga tcgaggtcga actggcgata atgagaacgg gtgaagcgcg
     6541 acgttacgcg ctcactcacc gtcgcatgct gcgtctcttc ctttaatacc ggaatttgat
     6601 cagcacgcgt gatatcttct acagcgaagg tctggcctgc tattgcaagc aggccagcta
     6661 acgcggtaag cctaaaaaac atgttcatgc ctggcccggc ctccgtttca gaacaccagg
     6721 tgttctgcgc gcacaatcaa agacataccc gaattcagct ggacgcggac gccgtctttg
     6781 gtgatttcta atacggtggc atccatcgcg ttttgacccg ctttcacctt cagggcttgt
     6841 ccgacagtca gagctgaaat gtcagaaacc ggggtgtgct gttcttcgcg aggtgctttt
     6901 actgtttttg gcgctttctc taccggcttt tgcgaacgag gtttacgttc agcgccttct
     6961 ttgcggcgtg gcgtggtcgg acgtggcttg cgttcgcggc gcggtgcgtc ttctttctca
     7021 ccagcagctg cggcagcttc gcgttttttc gcttgctgtt cagcacgctg tgcctgaaca
     7081 cgcgctttcg cttcttcaag ctgcttgcga gcatgctcga catgttgctc gtccagctca
     7141 ccgcatgggt tgccgtcaag atcgacacgc gttgcgccgg gtttaacacc gtaaagataa
     7201 cgccagctcg aagtgtagag acgtaaagcg gatcgcaatt gcgttttgct caggttcatt
     7261 tccccggcaa cacgatcgac caaatcctga aaaataccga ttttcagcgg acgcgcttca
     7321 ccttccgcac tgaaacagtg gggaaaacgt tcggccagaa acgcgattac ttctttactg
     7381 ctattcaact taggttgatt ttccatgaaa tttcctgatt acaacggacg tagccaacaa
     7441 gcgcaggcat gaacaggcgt cattataatg acgctatcag taaatgctac gttatccgtt
     7501 gattatcctg cgacgctcgc aaagaatttt ttgtaatccg tcgttgcaag cactttttca
     7561 agctgtgcca caagctgacg taagccttgc tcgtcctcgt ctgtaaagcg accgaagacg
     7621 gtactatcga tgtcgagaac accaataatc tgatttttca ccaccagtgg cagaacaatt
     7681 tcagaattac tcgccgcatc acaggcaata tgcccgtcaa acgcgtgaac atcctcgata
     7741 cgctgcactt gattgcgggc aaccgcagtg ccgcatacac cgcgctcaac gggtatccgg
     7801 acacaggcaa ttttgccctg aaatggtccg agcaccagtg tatcgtcctc aagcaaataa
     7861 aaacctgccc agtttacgtc agtgagacgc tcatataaca acgcactggt gttcgcaagc
     7921 gttgccagaa aactggtttc tcctgccatc agcgcgttaa agtcgcgatt taaatccgcg
     7981 taaaattctg ctttgttcat tatataatca cttggttgtc ttacctggaa ctgccagcct
     8041 attaaaataa gcattaaatg cgttaatgct caagatcatt cccatcatgg gttaagatta
     8101 atgttaattc ttattacatt tggcacgtca tggctcttaa cacaccacaa attacgccga
     8161 caaagaagat aacagtgagg gctatcggcg aggaactgcc gcgtggtgat taccaacgtt
     8221 gcccgcaatg tgacatgctg tttagcctgc ccgagataaa ttctcatcaa cgtgcctatt
     8281 gtccgcgctg tcaggcaaaa attcgtgacg ggcgcgactg gtcgctaacg cgcctggcgg
     8341 caatggcctt caccatgctg ttgcttatgc cgtttgcctg gggcgaaccg ctgttgcata
     8401 tctggctgtt aggcattcgt attgacgcca acattatgca aggcatctgg caaatgacca
     8461 aacagggcga tgcgataacg gggtcgatgg tctttttctg cgttatcggt gcccccctca
     8521 ttctggtgac ctccatagct tatttatggt ttggtaaccg attgggaatg aatttacgcc
     8581 cggtactgct gatgcttgag cggctgaaag agtgggtgat gttggatatc tacctggtcg
     8641 gcattggcgt tgcttctata aaggtacagg attatgccca tatccaggcg ggggtcggct
     8701 tgttctcttt tgtggcgttg gtgattttaa cgacggtgac gttgtcacat cttaatgttg
     8761 aagaactgtg ggagcgattt tatccgcagc gccccgctac gcgtagggac gagaaactcc
     8821 gtgtctgtct tgggtgccat tttaccggct atcctgatca gcgtggtcgc tgcccgcgtt
     8881 gccatatccc gctacgcctg cgtcgccgtc atagtctgca aaaatgctgg gcggcgctgt
     8941 tagcgtcaat cgttttgttg ttacctgcca acctgttgcc tatctctatc atttatctga
     9001 atggtggtcg gcaggaagat acgattcttt ccggaattat gtcgctggca agtagcaaca
     9061 ttgcagttgc gggaatcgtg tttatcgcca gtattctggt accgtttact aaagtgatcg
     9121 tcatgttcac tttactgttg agtattcatt ttaaatgcca gcaaggttta cgcacacgca
     9181 ttctgttact gcggatggtg acctggattg gtcgctggtc gatgctcgac ctgtttgtca
     9241 tatctttaac catgtcgctg attaatcgcg atcagatcct cgcttttact atgggaccgg
     9301 ctgcgtttta tttcggcgca gcggtaattt tgactattct tgctgtggaa tggctggaca
     9361 gccgcttact ttgggatgca catgagtcag gaaacgcccg cttcgacgac tgaagcgcag
     9421 attaaaaata aacgccgtat ctcacctttc tggctgctgc cgttcatcgc gctaatgatt
     9481 gctggttggc tgatttggga cagttatcag gaccggggca atactgtcac catcgacttt
     9541 atgtcggcgg atggtattgt tccaggccgt acgcctgttc gttatcaggg cgttgaagtc
     9601 ggaacagtgc aggatatcag cctcagcgac gatcttcgta agattgaagt caaggtcagc
     9661 atcaagtccg atatgaaaga tgcgctgcgc gaagagacac agttctggct ggtgacgcca
     9721 aaggcctcgt tggcaggtgt ctccgggctg gacgccctcg tcggtggtaa ttatatcggc
     9781 atgatgccgg gtaaaggtaa agagcaggat cactttgtcg cactcgacac ccaaccgaaa
     9841 tatcggctgg acaatggcga tctgatgatc cacctgcaag cccccgatct cggttcactg
     9901 agcagtggtt cattggttta tttccgcaag atcccggtgg gtaaagtcta cgactatgcc
     9961 atcaatccca acaagcaagg cgtggtgatt gatgtcctga tcgagcggcg ttttaccgat
    10021 ctggtgaaaa aaggtagccg tttctggaac gtttccggcg ttgatgccaa cgtcagtatc
    10081 agtggcgcga aggtgaaact ggaaagtctg gcggcactgg ttaacggtgc gattgccttc
    10141 gattcacctg aagagtcgaa acctgccgaa gcggaagata cctttggtct gtatgaagac
    10201 ctggcccata gccaacgtgg cgtaataata aaactggaac tgccagacgg tgccggatta
    10261 accgccgact cgacgccgtt aatgtatcag gggctggaag tcggacagct gactaaactg
    10321 gatttaaatc ctggtggtaa agtcactggg gaaatgaccg ttgatcccag cgtcgtcacc
    10381 ctgcttcgtg aaaatacccg catcgaatta cgcaacccga aattatccct tagcgatgcc
    10441 aatctcagcg ccctgctgac cggaaaaacc tttgagttgg tgcccggcga tggcgagcca
    10501 cgcaaagagt ttgttgttgt gccaggcgaa aaagccctgc tgcaagagcc ggatgttctg
    10561 acactgaccc tgacagcacc agaaagttac ggtattgatg ctggtcagcc gctcattctt
    10621 cacggcgtgc aggtaggcca ggttatcgat cgtaaactca ccagcaaagg cgtcaccttt
    10681 accgtcgcca tcgagcctca gcatcgcgaa ctggtaaaag gcgatagcaa atttgtcgtc
    10741 aacagccgtg tcgacgtgaa ggtggggctg gatggcgttg agtttctcgg tgccagcgcc
    10801 tcagaatgga tcaatggcgg aatacgtatt ctgccgggcg ataaaggcga gatgaaagcc
    10861 agctatccac tgtatgccaa tctggaaaaa gcgctggaga acagccttag cgatttaccc
    10921 accacaaccg tgagtttgag tgcagagacg ctgccggatg tgcaggcagg atcggtagtg
    10981 ctgtaccgta aatttgaagt tggtgaagtt attactgtgc gtccgcgagc taacgcgttt
    11041 gatatcgatc tgcatattaa gccggagtat cgcaaccttc tgaccagcaa cagcgtgttc
    11101 tgggcagaag gtggggcgaa agttcagctg aatggtagtg gtctgactgt acaggcatcc
    11161 ccgctctcca gagcattaaa gggggctatt agcttcgata acctcagcgg tgccagcgcc
    11221 agtcagcgta aaggcgacaa gcgtattctg tatgcttccg aaacagcggc ccgtgcggtt
    11281 ggcgggcaaa ttacgcttca cgctttcgat gccggaaaac tggcggtcgg gatgccaatt
    11341 cgctatctcg gtattgatat cgggcaaatc cagacgctgg atctgattac cgcacgcaat
    11401 gaagtacagg caaaggcggt gctctatccg gaatatgtcc agacctttgc tcgcggtggt
    11461 acgcgcttct cagtggtcac accgcaaatt tcggcagctg gcgttgagca tcttgatact
    11521 atcctccagc cgtatatcaa cgtcgaacca ggccggggca atcctcgccg cgactttgaa
    11581 ttacaagagg ccaccattac tgattcgcgt tacctggatg gcttaagcat tattgttgaa
    11641 gcgccggaag ccggttcgtt aggtatcggt acacctgtgc tgttccgtgg tctggaagta
    11701 ggtacggtta ccggaatgac gctggggaca ttgtcagatc gcgtgatgat tgcgatgcgc
    11761 atcagtaaac gctatcaaca cctggtgcgt aacaattccg tcttctggtt ggcatcgggt
    11821 tacagtctgg actttggtct gacgggcggc gtagtgaaaa ccggcacctt taaccagttt
    11881 atccgtggcg gcatcgcctt cgccacgcct ccgggtacgc cactggcacc gaaagcccag
    11941 gaaggcaagc acttcctgtt gcaggaaagt gaaccgaaag agtggcgtga atggggaact
    12001 gcgcttccca aataatgccc actgctccgg cgtgcctgcg ccggagcgtt tatgctaaac
    12061 tgcgcgcctg tttttttgcc agtggtatat gctcgtggcc caacacaccg tttatttccc
    12121 ggacgccttt ctgacacaaa tgcgcgaagc gatgccttcg acgctctctt ttgatgattt
    12181 tcttgccgcc tgtcagcgcc cgttgcgccg cagcattcgc gttaatacgc tgaaaacctc
    12241 cgttactgat ttcctgcaat taactgctcc ttatggctgg acgcttacgc caattccgtg
    12301 gtgtgaagaa ggtttctgga ttgaacgcga caatgaagat gcattgccgt tgggtagtac
    12361 cgccgagcat ttaagcggcc tgttttatat tcaggaagcc agttcaatgt tgcctgttgc
    12421 cgccttgttt gctgacggta atgcaccaca gcgggtgatg gatgtcgctg ctgcaccagg
    12481 ttccaaaacg acgcaaattg ctgcgcggat gaataacgaa ggggcaatcc ttgccaatga
    12541 gttttccgcc agtcgggtaa aagtgttaca tgccaatatc agccgctgtg gcatcagtaa
    12601 tgttgcgctc acgcattttg atggccgcgt atttggtgcg gcagtgccag aaatgttcga
    12661 tgccattttg ctggacgctc cctgctccgg cgaaggcgtg gtgcgtaaag atcccgatgc
    12721 gctaaaaaac tggtcaccag aaagcaatca ggaaatcgca gcgacccaac gggaactgat
    12781 cgacagcgcc tttcatgcat tacgtcctgg cggtacgctg gtttactcga cctgtacctt
    12841 aaaccgggaa gaaaacgaag ccgtttgcct gtggctgaaa gagacttatc ccgacgcagt
    12901 agagttttta ccgcttggcg atctcttccc tggtgcaaac aaggcgcgga ccgaggaagg
    12961 ctttttgcat gttttcccac aaatttacga ctgcgaaggc ttcttcgttg ctcgtctgcg
    13021 taaaactcag gcgattcccg ccttacccac cccaaaatac aaagtgggca atttcccgtt
    13081 cagcccggtg aaagatcgcg aagccggtca aattcgtcag gcggctgcaa gtgttggctt
    13141 aaactgggat gaaaacctgc gactctggca gcgtgacaaa gaactgtggt tgttcccggt
    13201 aggcattgaa gccctgatcg gtaaagtccg attttctcgc ctggggatta aacttgccga
    13261 aacgcacaac aaaggttatc gctggcagca tgaagccgtt attgcccttg cctcccccga
    13321 caatgtgaac gcttttgaac tgacaccgca ggaagcggaa gagtggtatc gcgggcgcga
    13381 tgtttacccg caagccgcgc cagtggcgga tgatgtattg gttaccttcc agcatcagcc
    13441 gattggttta gccaaacgga ttggttcacg actgaaaaac agctacccgc gtgaactggt
    13501 gcgcgacggg aaacttttta ccggtaacgc ctgacagcgc acaaaaaaag cgcacttttt
    13561 gactggcaca ttcaactgcc tcaactaagc tgaaaaatgg tgcgatcgga ctggtcgtac
    13621 cacaaccggc agctaaatgg agagcacgaa gatgaaaacc agtgtgcgca taggcgcttt
    13681 tgaaatcgac gacggcgaat tacacggcga atcgccgggt gatcgaacgt taaccattcc
    13741 ttgtaaatca gaccccgatt tatgtatgca actggatgcc tgggatgctg aaaccagtat
    13801 ccctgccctg ctaaatggcg aacactctgt cctttaccgt acccgttacg atcaacaatc
    13861 tgatgcctgg attatgcgtc ttgcctgatc caaaaagaac ccgtcggcat ggcgggttat
    13921 ttgtccgggt ttttcccccg ttgtaaaatc tctcctaaac ttaacggtac ggcaccacac
    13981 ttcggggatg aaatgttcgc gctggtactt tttgtttgct acctggatgg cggttgtgaa
    14041 gacattgttg tggatatcta caacacggaa cagcagtgtc tttattctat gagcgatcaa
    14101 cggatccgcc atggcggttg ttttccgatt gaggatttta tagatggttt ctggcgacct
    14161 gcacaggagt acggtgattt ttaattattg caattgcaca agagtcagtt ctcccccaaa
    14221 gacagcaccg gtatcaatat aatgcaggtt gccaatatcc acgcgatggc gcaaaggtgt
    14281 atgaccaaac cagaaatgat cagcacctgc aattccctgc cctttttggc gttcacctaa
    14341 tcgcgagcgg ctccacaaga cctgatgcaa atcgacgtcc ttttgccatt catagacatc
    14401 atctggataa tcggcatgag caataacatg tttgccggtg cgactgtgta attcaagaat
    14461 aaagggcaaa tgctgacatt tttccagcgc cgttttcgct tgtttctgtt gattatctgc
    14521 cagcgcaata aaccagtcgc cgccatttat caaccataaa gacatctgcc gggatgccag
    14581 cgcatccatc gccatctgtt catgattgcc tcttaccgaa cgaacccaat gttgttccag
    14641 taactgcagg caacgtaaac tttgcggccc acgatcgata acgtctccca ctgagataag
    14701 taaatctcgc cacggatcaa aacgacaatg ccataatttg cggcgcaact gctcaagaca
    14761 accgtgtata tcgcctgaaa gccagatatg tcgccattga tgacccgcaa ttctctgata
    14821 aacgggcgca ggctgtttca tcaatatttt cctcccgcgc taaagatcac ataatcttaa
    14881 caagaatgtt aaaaaacgct ggactcagac agtagagtgt gtgttatggt tgactataaa
    14941 gtcagcgaag ggaatgcttc tggcttttaa cagataaaaa gagaccgaac acgattcctg
    15001 tattcggtcc agggaaatgg ctcttgggag agagccgtgc gctaaaagtt ggcattaatg
    15061 caggcttagt tgccttgctc tttaagaata gatgacgacg ccaggttttc cagtttgcgt
    15121 gcaaaatggt caataaaaag cgtggtggtc atcagcttaa atgttaaaaa ccgcccgttc
    15181 gggtgaaaga actgaggcgg ttttttattg gaaatcaaaa ggctatttta ggtaattaac
    15241 agagtttttc agctcgctct ataaacggtg ccagactcat tttttcgccg ggattgttag
    15301 gatcatcaat ctgaatcacc gaaatgggtt gggctttagt cttcccactg gaaacttcct
    15361 tttgtgcgat atcgtttaaa ggatactgca cgagagtact tgggttaatg acatacaaag
    15421 cattacccgg tcggcaagtc agcatcacct cttcgcgatt aaacgcccat ttgtctttac
    15481 ccacttcaaa acggctgacg gtaatcacct gcggtgccgc cagcgccgct gcagaactgg
    15541 tgagtaacag aaacgccaga atactttttt tcatcatttt cgtaatccat caaaaaggtt
    15601 cgagagtcgc aagcaggctg atgatcacca gcactaccgc acctatcgcc cattcgagtt
    15661 tagtcatcca cacaaaccag ggagccgcac gatcttcatc ctgcctcatg cgcggtacaa
    15721 gaacataacg attagccagc gcgatgacca ccataatcat caccaggatg gctttgagca
    15781 ataagagttg gccccagtaa gtcgtaagcg tggggggaaa tccggtaatc aacaatgcat
    15841 taagcacgcc gcttgccagt acgccgatca ccgcaaaatg cccgcaccag gaaaaacgca
    15901 tcagcgcctg aatagcctgg tgtcgccaac ggcctttgat gagttgcata caccagagca
    15961 ccggcagtaa tcccccaaac cacgccgccg cacagatcag gtgaattgcg tgattagtct
    16021 gatggatttt cgccgttacc ccttcattca acgtcgcatg cccgactccc gccagcagaa
    16081 taaattgcgc ggtagtgagc ataaacagca aacgtggcat attccgcggt tgcataaggg
    16141 cgacgatcaa cgttacgagg gcgagaacaa tttgccataa ccagacgcca ccaaactgcg
    16201 tttgcaaaac agccaaccaa atgtttggcg aaaatacatc tgaccatccc gctcccatca
    16261 acccgccctg aacggcgagc attgcagtgg cactgatcaa actccagacg gctgcgtgct
    16321 gttgtaaacg cagaaaacgc ttcgtcagca agcgacgaat cgttaagggg gccagccagg
    16381 caccgtacat tgcgaagcca aaaaccagca tcagcgaggt gaaatggata aatcgcagcg
    16441 cgatccaggt aaacgccagc atgatttatt tcacactaaa ggtgtaatgt cctttcgttt
    16501 tatgcccatc caccgaaaca acatgccagt caacggtgta ggttccgggt ttcagcgaat
    16561 cggccagtgg aacaatcagt tgcttttggt cctgctcatt ccgcttcgcc ggtaatgttt
    16621 taatattttc gttttttggc cccgtgattt ttgcaccact gaatcctgtt tcaacgcctt
    16681 ccgagaagtt taaagtgatt gcctgcggag ctgctgtcac ttgtgcattt gccgcaggat
    16741 actgatgcgt taaatgcgca tgtgcccaga cggaaggtgt caccaacgaa gtggtcagga
    16801 ttgccagcgc gtagcgaagg gagcgtgcag ttgaagccat attatctatt cctttttata
    16861 ataacttttt tacagagcat aaccttgtct aatgtctgag tcgaggatca tcaattccgg
    16921 cttgccatca aggctcactc ttagtaactt ttgcccgcga atgatgagga gattaagaat
    16981 gctgaagaat ctggctaaac tggatcaaac agaaatggat aaagtgaatg tcgatttggc
    17041 ggcggcaggg gtggcattta aagaacgcta caatatgccg gtgatcgctg aagcggttga
    17101 acgtgaacag cctgaacatt tgcgcagctg gtttcgcgag cggcttatcg cccaccgttt
    17161 ggcttcggtc aatctgtcac gtttacctta cgagcccaaa cttaaataaa acttatacat
    17221 agttacactt tcttacataa cgcctgctaa attatgagta ttttctaaac cgcacccata
    17281 atttgcagtc attttgaaaa ggaagtcatt atgtcgttct ggaaagttgc agcagcgcaa
    17341 tatgagcccc gtaaaacctc actcaccgaa caggtagctc atcatctgga gtttgtgagg
    17401 gccgccgcca ggcaacaatg tcagctgtta gtttttccat cgctttcttt actggggtgt
    17461 gattattcgc gacgtgccct tcctgcccca cccgatctct cgctattaga cccgctttgt
    17521 tatgccgcaa cgacctggcg aatgaccatc attgccggcc ttcccgttga atataacgat
    17581 cgctttattc gtggaattgc ggtgttcgca ccgtggcgaa aaacgccagg gatctaccat
    17641 cagagttatg gtgcatgtct gggccgtcgt tccagaacca tcacggtggt cgatgaacaa
    17701 ccgcaaggta tggatatgga cccgacctgt tcactgttta cgacagggca atgtctggga
    17761 gaacccgacc tgctggcgtc ggcccgtcgc ttacaatttt tttcacatca gtactcgatt
    17821 gctgtgctga tggcaaatgc ccgtggtaac agtgcactat gggatgaaca tggtcgtctc
    17881 atcgttcgcg ccgatcgtgg ttcattattg ttagtcggtc agcgttcatc acagggttgg
    17941 caaggcgata tcattccatt acgctaggct tttcggcctg gagcatgcca tgttgcgcat
    18001 tatcgataca gaaacctgcg gtttgcaggg agggatcgtt gagattgcct ctgttgatgt
    18061 cattgacgga aaaatcgtca atcccatgag ccacctggtg cgtcccgatc gtcctattag
    18121 tccacaggcg atggcgattc atcgcatcac cgaagccatg gtcgccgata aaccgtggat
    18181 tgaagatgtg atcccgcact attacggtag tgaatggtat gtcgcgcata acgccagctt
    18241 tgaccgccgc gtactgcctg aaatgcccgg tgagtggatt tgcactatga aactggcccg
    18301 tcgtttgtgg ccagggatca agtacagcaa tatggcgcta tataaaacac ggaagctcaa
    18361 tgtacagaca ccgccgggcc tgcatcatca ccgcgcgttg tatgactgtt atatcaccgc
    18421 cgcgttgctt atcgatatta tgaacacctc tggctggacg gcagaacaga tggccgatat
    18481 caccggacgt ccatcgttga tgacgacctt cacctttggc aaataccgtg gcaaagcggt
    18541 ttccgacgtt gccgaacgcg atccgggcta tctgcgctgg ttgtttaata acctcgacag
    18601 catgagcccg gagctgcgtt taacactgaa acattatctg gaaaacactt aatccgctga
    18661 ctgagcgggt aatgttccct gcgccagcgc gaccaaaaaa gcatattcca tcgctacgcc
    18721 ttcgtacgat ttgaagcgac cagatttacc gccatgacct gagtccatgt cggtacagag
    18781 caataaaaga tggtcatcgg ttttcagctc gcgcaattta gcgacccatt ttgccggttc
    18841 ccaatattgc acctgagaat cgtgtaaacc ggtcgttacc agtaaatgcg gataagcctg
    18901 tgcggtgacg ttgtcatacg ggctgtagct tttcatatac tcgtaatatt gcggatcctg
    18961 cggattcccc cactcttcaa attcgccggt ggtaagagga attgattcat ccagcattgt
    19021 tgttacaaca tcaacaaacg gtacctgggc gataacgccg tggaacaatt ccgggcgttg
    19081 attaatcgca acgcccatca acataccccc cgcactcccg cccatcgcat aacagagcga
    19141 aggagagcca tagcccagtt ttaacaatgc atcgcaggca tcaagataat cattaaacgt
    19201 atttttcttc ttcagaaatt taccgtcctc gtaccattgt tgccccagct caccgccgcc
    19261 acgaacatgg acaatggcgt agacaaatcc acgatccaac aaactcaagc ggctaaaact
    19321 gaaatcggca tcaatacttg cgccgtaaga accgtagcca tacaccagca gcgggttgtg
    19381 tcctttgcga aaatgtttgc gatgatagac cagcgaaacc ggaacttcga cgccatcacg
    19441 ggcgactatc cacaggtgtt cactgcggta attcgccgca tcaaaaccag gaacttccgt
    19501 ttgttttaat acacgacgct caccggtatc catatccagt tcaaacaaag tatctggtgt
    19561 ggtcatggac gaataaccat aacgcaatcg cgcggtttca ggttctggat tgtaggcaat
    19621 ccaggtcaca taggccggat catcaaaggc aatgccaatg acttctcggg tcttgcggtt
    19681 aatttgccgc aaactggtta acccgcgctg acgctcttca accaccagcc agtcggtaaa
    19741 cagcgtaaac ccttccagca taatgttatc acgtggcgga attaactctt cccactgttg
    19801 ctcatcacgc atacgggtac ggtataagcc aaagttttta ccgtggcggt tggaacgcag
    19861 ataaaaccga tgctggtagt gatcaaggct gtattcgtga tctttgcggc gaggcagaaa
    19921 aacaaacggc tcggcatcgg ccatttccgc gtccagcagg cgaacttcac tggtggtggc
    19981 gctggccaga tgaatgacca cataatgctt cgaggtcgtt ttatgcaggc tgacgtaata
    20041 ggtatcgtct ttttcttcgt aaatcagttt atcttgcgat gctggcgtac cgatggcgtg
    20101 acgccagacc tgataaggca gcagcgtcac tggatgcttg cgaacatagt agaaagtcca
    20161 ggagtcattt gcccagacaa agctgggttc aacgttatcc agcagttccg ggtaccagtt
    20221 gcctgtttcc agattacgaa aacgaatgcc gtactgccgt cgggaaagaa aatcttctgc
    20281 cagtgccata atggtgttat ctggcgtaat cgccattccg cccatcgaat aaaactcact
    20341 atgagccgcg cgcttgttgg catcgagcaa tgttttccac tcgtcccact cttcactgaa
    20401 cgccgattga cgctggtaga tagcatattc acagcctggt tcataaatat gccgatagcg
    20461 gtagccattt ttgatgtagg gcgcagaaac ttctcgttgc ggaatgcggt cgatgatttc
    20521 ctttaagatg cgatcctgca aggcttgttg cgaggccatc acccgatgac cgtaactatt
    20581 ttcttgttgc agatagtcca ggacttccgg ctgagaacgc gtatcgtccc gcagccagta
    20641 gtaattatcg atgcgcgtat cgccatgaag cgtcatggcg tggggaatgc gggcggcttt
    20701 tggtagcatg ttattgttct ttctggttga aacatcttat aagggtggca aaactcaccg
    20761 gggatgcaag cgaaacaggg tagtcattgc ttagatgatg acaggtaatg gcgcggatat
    20821 cgactgttat gcagacagag aaaatcagcc tgttcaaatg gttgtgcggt tctggataac
    20881 ccgaaatagt caacttcagg ctatccagag agcggaatta ctccgccaaa gtacgttttt
    20941 gctgttcgag atcgcgttca atgccttcac gaacgtcctg ggggattttc agcgcatcac
    21001 ccagtgcatt caggtaactg cgttccataa aatggtcaat atcaatagcc gcgcagctca
    21061 ggaaatagat ttccagcgcc tcttcttcat tgcggacccc ggtagccagg cgttgtggat
    21121 ccagcggttg ttcgattgcc tgctcaatga gtacacgccc cttctcttcc acaccggctt
    21181 ctcgcaattg ctggtcgatt gccgcacgtt ctttggcatc gatatggcca tcacttttag
    21241 cggcaaagac cagcgcaagg atcaaacgtt ccgtacgctc atccagcggc gtactttgcg
    21301 cgccaaactg cggttcatcc tgatgcgccg cgcgaatttt atctttgtat ttattccaca
    21361 gcaccgtacc cgctaccgct ccgccgccaa ccagtaacgc gtttgtgcca tatttcgtaa
    21421 gaagtttacg tgctgattta tttgcgacca gcagcccagc cagtccgcct aatgcgccag
    21481 gcactaaaag tttgcccaat ccctgatccg cagacgagga ggtagaagaa cggctttgcc
    21541 caagcagaga ttgcagttga tttaaccagt tagccatatt tgctcctcaa taaccatttt
    21601 tagtgcctgt agcatagcgg atgaggatgt cagaaaatgt atatcgagac aaaagatgcg
    21661 caaataagcg gggcggtagg ccgttattca aagaaattcg cgccaggtaa ggtgttcact
    21721 gtatataagg tgataagggt attttaggtg aaagtagttt gacattttga gtggaactgt
    21781 ttgatgctga ttgcccagtt cgacgctact gcgtcttctc aggactacga ttgtgttgcg
    21841 ttgtagggcg gacaaggcac tcgcgccgta tccgccaacg gtcaatcgaa ttatcagaag
    21901 ccattaacgc cgctgatatt ccgccattcc cgctttgcag tccacgctga cctggtaatg
    21961 aatatcggca cttttaccac ggacggttag cggtactgac catttatcat ctttcccctg
    22021 aatgtcctgc aaactgaccc atgccacggg gtcggcctga ccgacaattt tttgatcatc
    22081 agcccagcgc gccacgcgat tttgttggta atcacgtttt acgctcgcgg caattccggc
    22141 agcatccaga ccttcacact ttgggaaagt gaccgacttg ctggtttcat tattggcagc
    22201 gaaaactgat gcgcaggcag aaaccaacaa cagccctaaa aacgcccctc tttttttcat
    22261 gtttttctcc atagcacaat gattcaggag aaagcatggt acaaattgtc aggagcgcaa
    22321 gttgcttcag gccgcgtgtg aggcatcttc ctcttcggat tcagcaccga cgacctgtgg
    22381 ggacggtaat ttgccggttt tcagaatggt gctcagcaca tctttttgtt ctgccagcca
    22441 tagcgaaagg gcttcacgtt gctcatcttc catttgcact ggagaatttg tcagccaggt
    22501 gtcaagcaga tcggccgtat caagcatctt gtcataagca tcggcttcct ttttgctggt
    22561 aaacgacatt ttctcttcgc cctcacgaat gactacgtat ttaacttcaa ccgccatttg
    22621 cagcctctca taataactgt gattttatac agtatatttc ttttcagttg ggaaatcaac
    22681 atcagcaata aagacacacg caaacgtttt cgtttatact gcgcgcggaa ttaatcaggg
    22741 gatattcgtt atgacgttat taggcactgc gctgcgtccg gcagcaactc gcgtgatgtt
    22801 attaggctcc ggtgaactgg gtaaagaagt ggcaatcgag tgtcagcgtc tcggcgtaga
    22861 ggtgattgcc gtcgatcgct atgccgacgc accagccatg catgtcgcgc atcgctccca
    22921 tgtcattaat atgcttgatg gtgatgcatt acgccgtgtg gttgaactgg aaaaaccaca
    22981 ttatatcgtg ccggagatcg aagctattgc caccgatatg ctgatccaac ttgaagagga
    23041 aggactgaat gttgtcccct gcgctcgcgc aacgaaatta acgatgaatc gcgagggtat
    23101 ccgtcgcctg gcggcagaag agctgcagct gcccacttcc acttatcgtt ttgccgatag
    23161 cgaaaacctt ttccgcgaag cggttgccgc cattggctac ccctgtattg ttaaaccggt
    23221 gatgagttct tccggtaaag ggcagacgtt tattcgttct gcagagcaac ttgctcatgc
    23281 atgggagtac gctcagcaag gtggtcgcgc cggagcgggc cgcgtaattg ttgaaggcgt
    23341 cgttaagttt gacttcgaaa ttaccctgct aaccgtcagc gcggtggatg gcgtccattt
    23401 ctgtgcaccg gtaggtcatc gccaggaaga tggcgactat cgtgaatcct ggcaaccaca
    23461 gcaaatgagc ccgcttgccc ttgaacgtgc gcaggagatt gcccgtaaag tggtgctggc
    23521 actgggcggt tatgggctgt ttggtgtcga gctgtttgtc tgtggtgatg aggtgatttt
    23581 cagtgaagtc tcccctcgcc cgcatgacac cgggatggtg acgctaattt ctcaggatct
    23641 ctcggagttt gccctgcatg tacgtgcctt cctcggactt ccggttggcg ggatccgcca
    23701 gtatggtcct gctgcttctg ccgttatcct gccgcaactg accagtcaga atgtcacgtt
    23761 tgataatgtg cagaatgccg taggcgcaga tttgcagatt cgtttatttg gtaagccgga
    23821 aattgatggc agccgtcgtc tgggggtagc actggctact gcagagagtg ttgttgacgc
    23881 cattgaacgc gcgaagcacg ccgccggaca ggtaaaagta cagggttaaa cccggcaaaa
    23941 aaacgctaca aaaatgcccg atcctcgatc gggcattttg acttttacag cttagcgcct
    24001 tctacagctt cacgcgccag cttagtaatg cggtcgtaat cgcccgcttc cagcgcatct
    24061 gccggaacca gccaggaacc accgatgcac agcacgcttt tcagcgccag gtagtcacgg
    24121 tagttagccg gagaaatacc acctgtcggg cagaaacgga cctgggagaa cggacccgcg
    24181 atcgcctgca gggctttcac accgccgtta gcttcagccg ggaagaattt gaactctttc
    24241 aaaccgtagt ccatacccag catcagttcg gaaacagtgc tgatccccgg aatcagagga
    24301 atagtccctt cggtagcagc tttcagcagc ggctcggtca gacccgggct aattgcaaac
    24361 tgtgcacccg cttcagtgac ttctgccagc tgctgtggat tcagcaccgt accggccccc
    24421 acaatcgctt caggcacttc tttggcgata gcacggatag cgtcaactgc gcactcggta
    24481 cgcagagtca cttccagaac gcgcacccca ccagcaacca acgcttttgc catcggcacc
    24541 gcatgttcca gtttttttac cacgataacc ggtacaaccg ggccggtggt caggattgat
    24601 tctgcacttg ttttccagtt tttcatcaga gttttctctc gcctgattac aaatttgtcg
    24661 tcttaaaaag tgatacaggt tgcgccctgt tcggcaccgg acagtttttc acgcaaggcg
    24721 ctgaataatt cacgtcctgt tcccacgcgt gacgcgctca ggtcaggaat gtgcggttcg
    24781 cgagcagcca gttccgcttc gtctaccagc agcgtcagtt cgcctgtctg tccattcaca
    24841 cgaatgatgt ccccgtcgcg cacttttgcc agcagcccgc catcgtaggc ttctggtgtt
    24901 acgtggatag ctgacggcac tttacctgaa gcgccggaga gtcgtccatc ggtaactaac
    24961 gcaattttga aacaccggtc caataataca ccaagtggcg gcatgagttt atgtaattct
    25021 ggcattccgt tcgcttttgg cccctgatga cggacaacaa cgacacaatc gcggtccagt
    25081 aaacccgctt caaaggccgg cataacgtca tgctggcttt caaaaacaac cgctggcgct
    25141 tcaatcacct ggttctctac cggcacggca gaggttttca taaccgctcg gcccaggtta
    25201 ccgcttaaca cttttgtccc accatgatga gagaaaggtt gttcgaagga cgcgatcaca
    25261 ttgttgtcga gtgatttttc tgccccttcc cgccagtcca gttcaccatt attcagccat
    25321 ggttcaaggg tataacgaga cagaccaaag cctgccaccg tattgacatc ttcatgcagc
    25381 aggcctgctt tgagcagttc acgtaccaga accggtacgc cacctgccgc ctggaagtgg
    25441 ttaatatcgg ccggaccgtt cgggtagaga cgtgccatca gcggtacaac atcagaaagg
    25501 tcagagaagt catcccagtt aatctgaata ccggctgcgc gcgccatcgc caccaggtgc
    25561 atggtgtggt tagtggaacc accggtcgcc agcagtgcaa cgataccgtt caccaccact
    25621 ttctcatcga tcatcttacc gatcggcatc cattcattac cattaccggt catgcgcgta
    25681 acctggcgcg cagccgcagc ggtcaaagca tcgcgcagcg gagaatccgg atgaacaaaa
    25741 gaagagcctg gcaactgcat ccccataaac tccaccacca tctggttggt gttggcagta
    25801 ccgtagaaag tacatgttcc cggcgcatga taagacgcgg cttctgactc cagtaatgcc
    25861 atgcggtcca ctttaccttc ggcataaagc tggcgaatac gcactttttc tttatttggc
    25921 aaaccgcttg ccatcggtcc agacggcaca aacaccgcag gcaaatgacc aaacgacagg
    25981 gctgccatgg tcagacctgg gacaatcttg tcgcacacac cgagaaacag agcaccatca
    26041 aacatgttat gggatagccc caccgccgca gacatcgcaa tcacttcgcg gcttaacagc
    26101 gacaattcca ttccatcctg cccctgggtg acaccatcac acatcgccgg aacaccgccc
    26161 gcaacctgac caaccgcatt cgcttcatgc agggctttac gaatgatttc cggatagtgt
    26221 tcataaggct ggtgagcgga gagcatgtcg ttataggagg tgatgatggc gatattgtta
    26281 cgcaacatgc ttttcaaaga ggctttgtct tctggctggc aggcagcgaa accgtgtgcc
    26341 aggttaccgc atgccaactg cgaacgatga acggtcgaag ttttcgcttg ttctatccgg
    26401 gcgaggtaag cagagcgagt ctcgcgcgaa cgttcaatga ttcgatttgt tacgcgtaac
    26461 aattgtggat tcataaaggc tcctgaaatt gagttgtcag agcaggatga ttcacaacac
    26521 gtttcattca gaggattcat gactgaaacg cctgtaaccg gagctcatag ggcaaaaacg
    26581 tttcagtcag tgtaataaaa aaagcctcgt gggtgaatcc gcacgaggcc tgaaagtgta
    26641 aaaattgttc tacaatctgt gcaagatcat gttaccggta aaataaccat aaaggataag
    26701 cccagatatt actcaaactc attccaggaa cgaccgtcac gggtaatcat cgccaccgag
    26761 gcaacggggc cccaggttcc ggcctgatac ggtttcggcg catcattgtc catcgcccac
    26821 gcctcagtaa tggagtctac ccatttccag gcttcttcca cttcatcacg acgtacaaac
    26881 agtgcctgaa taccacgcat ggtttccagc agcagacgtt cataggcatc tgccagatgc
    26941 gtttgattaa aggtttctga atagctcaga tccagcttgg tgatttgcag gttatgtttg
    27001 tggtcaaggc caggaacttt attcagtacc tggatatcca cgccttcatc aggttgcaga
    27061 cggatagtca gtttattctg cggcagatcc tgccacgact ctttaaacag attcagttca
    27121 ggtgttttga aatagaccac gacttcagaa catttggtcg gcagacgttt accagtacgc
    27181 aggtagaatg gcacaccggc ccagcgccag ttatcaatgt cgacgcggat cgccacgaag
    27241 gtttctgtat tgctgctctt gttcgcgccc tcttcttcca gatagcccgg cacttttttg
    27301 ccctgagcga agcccgcagt atattgcccg cgtacggttt tttcgcgcac gttggaacgg
    27361 tcgatgcggc gcagagactt cagtactttc actttttcat cgcggatgct gtctgcgctc
    27421 aggtcagacg gcggagacat cgcaatcatg caaagaattt gcagcaggtg gttctggatc
    27481 atgtcgcgca tctgaccggc tttatcaaaa tagccccagc gcccttcgat ccccacttct
    27541 tctgccacgg taatctcaac atgatcaatg gtgcgattgt cccagttgtt cacaaacaga
    27601 gagttagcaa aacgcagcgc caacaggttc agcactgttt ctttaccaag atagtggtcg
    27661 atacggtaaa cttggcactc ctcgaagtat tcgccaacct gatcgttgat ttcctgcgag
    27721 gtcgccagcg acgtccccag cggtttttcc atgactacgc gtgccggttt agcattcagt
    27781 tttgcctcac caagcccttt gcaaattgcg ccaaaagtgc tgggcggcat ggcaaagtag
    27841 ttaatggtga tacgattttt ttgatccagc atcgcgccga gacggctgaa tgcagcagtg
    27901 tcattgacat caagattaca aaaatccaga cgtgcactca gggtgtccca taaaccttca
    27961 tcaatggttt ctttcatgaa agtttcgagc gcctcgcgga caactttggt ataagccgct
    28021 ttatcccagt cagcacgccc tacgccgata atccgggtgt ccgggttgag ctgaccggct
    28081 ttttccagtt gatacaggga aggcagcaat ttacgacgcg caaggtcgcc tttcgcgccg
    28141 aaaatgacca ggtcacaggc ctgggctgtt tgcgttaccg ccatgtcatt ctccttaagt
    28201 taactaaccc ggtacttaag ccagggtata cttgtaattt tcttacggtg cactgtactg
    28261 cttttacgag cttgcgaaaa ctgtaaacgc ttatccaccc gtgcgattac ggtaaaagcg
    28321 cgcaaagtgc ggcaaaactg ataaaaaaat catcgttttc cattgggtaa aaatctgaca
    28381 ctgatcatgt tatgaaaaaa aataacaact tttttatctg cttttgtcat taacacagca
    28441 cacaggcgta atatctgaca aaactgcatt tcgatttctt tcagagcgga aatcgtcatt
    28501 acccgtgagt ctctttacat catgaatatg ctggaaaaaa tccagtctca gctggaacat
    28561 ttgagcaaat ctgagcgcaa agttgccgag gtcattctgg cttcgcccga taacgcgatc
    28621 cattcgagta ttgctgctct ggcgctggaa gccaatgtta gcgaaccgac ggtgaatcgt
    28681 ttctgtcgca gcatggacac acgcggtttt cctgatttta aacttcatct ggcacaaagt
    28741 ctggcgaatg gcactcccta tgttaatcgc aacgtcaatg aagatgacag cgttgaatca
    28801 tacacgggga aaatatttga gtccgcaatg gcaacgctcg atcatgtccg tcattcactg
    28861 gataaatctg ccatcaaccg cgccgtcgac ttgctcactc aggcaaaaaa aatcgccttt
    28921 ttcggattag gttcttcagc cgccgttgcc cacgatgcga tgaataagtt ctttcgtttt
    28981 aatgttccgg tggtgtactc cgatgatatc gtgctgcaac gcatgagttg tatgaattgt
    29041 agcgacggag acgtggtggt gctgatttct cacacgggaa gaacaaaaaa tctggtcgag
    29101 ctggcgcagc tggcacgcga aaacgacgct atggtaattg ccctcacctc tgcgggtacc
    29161 ccgctcgccc gggaagcaac gctggcaatt accctcgacg taccggaaga tactgacatt
    29221 tatatgccca tggtttctcg acttgcacag ctgaccgtga tagatgtgct ggcgacagga
    29281 tttactttgc ggcgcggtac aaaattcaga gataacttga agcgggtcaa ggaagcgctg
    29341 aaggaatcgc gttttgataa gcagttactt aatttaagtg acgatcgcta aaaacgactg
    29401 tcactgtcct aatcttatac gacatccgaa tgagattaat ttatcgccat cgcggcgtta
    29461 tttcattcgg atttcatgtt caagcaacac ctggttgttt cagtcaacgg agtattacat
    29521 gtccagaagg cttcgcagaa ctaaaatcgt taccacgtta ggcccggcaa cagatcgcga
    29581 taataacctt gaaaaagtta tcgcggcggg tgccaacgtt gtacgtatga atttttctca
    29641 cggctcgcct gaagatcaca aaatgcgcgc ggataaagtt cgtgagattg ccgcaaaact
    29701 ggggcgtcat gtggctattc tgggtgacct ccaggggccc aaaatccgtg tatccacctt
    29761 taaagaaggc aaagttttcc tcaatattgg ggataaattc ctgctcgacg ccaacctggg
    29821 taaaggtgaa ggcgacaaag aaaaagtcgg tatcgactac aaaggcctgc ctgctgacgt
    29881 tgtgcctggt gacatcctgc tgctggacga tggtcgcgtc cagttaaaag tactggaagt
    29941 ccagggcatg aaagtgttca ccgaagtgac cgtcggtggt cccctctcca acaataaagg
    30001 tatcaacaaa cttggcggcg gtttgtcagc tgaagcgctg accgaaaaag acaaagcaga
    30061 cattaagact gcggcgttga ttggcgtaga ttacctggct gtctccttcc cacgctgcgg
    30121 cgaagatctg aactatgccc gtcgcctggc acgcgatgca ggatgtgatg cgaaaattgt
    30181 tgccaaggtt gaacgtgcgg aagccgtttg cagccaggag gcaatggatg acatcatcct
    30241 cgcctctgac gtggtaatgg ttgctcgcgg cgacctcggt gtggaaattg gcgatccgga
    30301 actggtcggc attcagaaag cgttgatccg tcgtgcgcgt cagctaaacc gcgcggtaat
    30361 cacggcgacc cagatgatgg agtcaatgat tactaacccg atgccgacgc gtgcagaagt
    30421 catggacgta gcaaacgccg ttctggatgg tactgacgct gtgatgctgt cggcagaaac
    30481 tgctgctggt caatacccgt cagaaactgt tgcagccatg gcgcgcgttt gcctgggggc
    30541 ggaaaaaatc ccgagcatca acgtttctaa acaccgtctg gacgttcagt tcgacaatgt
    30601 ggaagaagct attgccatgt cagcaatgta cgcggctaac cacctgaaag gcgttacggc
    30661 gatcatcacc atgaccgaat cgggtcgtac cgcgctgatg acctcccgta tcagctctgg
    30721 tctgccaatt ttcgccatgt cacgccatga acgtacgctg aacctgactg ctctctatcg
    30781 cggcgttacg ccggtgcact ttgatagcgc taatgacggc gtagcagctg ccagcgaagc
    30841 ggttaatctg ctgcgtgata aaggttacct gatgtctggt gacctggtga ttgtcaccca
    30901 gggcgacgtg atgagtaccg tgggttctac taataccacg cgtattttaa cggtagagta
    30961 agtacgttgc cggatgcggc gaaaacgcct catccggcct acagttcaat gataattcaa
    31021 cagatttcga atcttctgaa gcaaacttga acttatcatc agtcgaaggc ctctcctcgc
    31081 gagaggcatt ttttatttga tgggataaag atctttgcgc ttatacggtt ggatttcgcc
    31141 cggtttgcga gttttcagca attttaatat ccaggtgtat tgttctggtc gcggaccaac
    31201 aaaaatctcg acttcttcat tcatccgccg cgcaatcgta tgatcatccg cctctaacag
    31261 atcatccatc ggtgggcgca cctgaatcgt cagacgatgc gtcttgccat cataaatcgg
    31321 aaatagcggt acaacgcgcg cacggcacac tttcatcaaa cgaccaatcg cgggcaacgt
    31381 cgctttatag gtggcaaaga aatcaacaaa ttcgctgtgt tctgggccat gatcctgatc
    31441 gggtaaataa tatccccagt aaccctgacg taccgactgg atgaatggtt taataccatc
    31501 atttctcgca tgcagacgac caccaaagcg acggcgcacc gtgttccaga cataatcaaa
    31561 aaccgggttg ccctgattat ggaacatcgc tgccattttc tgcccttgcg aggccatcag
    31621 catggcagga atatcgacgg cccaaccgtg cggcaccaga aaaatcactt tctcgttatt
    31681 acgccgcatc tcttcgatga tctccagccc ttgccagtca acgcgcggct gaattttctc
    31741 cggcccgcgt attgccaact cagccatcat taccatcgct tgcggcgcgg tggcaaacat
    31801 ctcatctaca atcgcttcgc gttcagcttc actacgttct ggaaagcaga gcgacagatt
    31861 gattaacgca cgacggcgtg agctttttcc cagtcgtccg gcaaaacgtc ccagccgtgc
    31921 cagaatggga tcacggaact ttggcggcgt taaagcgata cccgccatcg ctgctacgcc
    31981 cagccatgct ccccagtagc gcgggtggcg aaaggattta tcaaactcag gaatgtattc
    32041 gctattattt tttttcgttt ccatgctttt ccagtttcgg ataaggcaaa aatcaatctg
    32101 gtgatagtgt agcggcgcaa cttgccccgc accaaataaa aaagccggta ctgactgcgt
    32161 accggctgcg aatggatgtt aattaatcaa accgtagctg cggcacaatc tctttggcct
    32221 gtgccaggaa ttcgcgacga tcggagccgg tcagcccttc ggtacgcggc agttttgccg
    32281 tcagcgggtt tacggcctgc tggtttatcc atacttcata gtgcagatgc ggcccggttg
    32341 aacgtccggt attaccggaa agcgcgatac ggtcgccacg tttcaccttc tgtcccggtt
    32401 tcaccaagat cttgcgcaag tgcatataac gcgtggtgta gctgcgacca tgacgaatag
    32461 ccacataata acctgctgcg ccactacgtt tggcaaccac cacttcaccg tcacccactg
    32521 aaagcactgg cgtaccttgt ggcatggcaa aatcaacacc tctgtgtggc gcaacgcgac
    32581 cggtcaccgg attagtacga cgcgggttaa agttagatga gatacggaac tgtttcgccg
    32641 tcgggaatcg caagaatcct ttcgccagac cagtaccgtt acgatcgtag aatttgccat
    32701 cttcagcgcg gattgcgtaa taatctttac cttctgaacg caaacgtacg cccagcagct
    32761 ggctttgctc acgtttacca tcaagcattt ctcgtgacat taacaccgca aattcatcgc
    32821 cttttttcag tttgcggaaa tccatttgcc actgcatggc tttaatcact gcgctcactt
    32881 cggcgctggt taaaccggcg tttctggcgc tggcaacaaa gcttcccccg acggtacctt
    32941 tcagcagatt gttgacccac tctccttgct gcatttcgct ggtcatttta aaaccgttag
    33001 cggcagtacg gtcataggtt cgggtttcac gacgagacac ttcccaggtg aggcgctgca
    33061 gttcgccgtc cgcggttaat gtccaggaga gttgttgacc gattttcagg ttacgcaatt
    33121 ctttgtcggc agcagccagt tgggtgatat cacccatatc aataccatac tgattgagaa
    33181 tgctgcttag cgtatcgcca gtggaaacaa catattcatg cacgcccgct tcaccggcga
    33241 ttttgtcatc cagttcgtcc tggggaatgg cttcatcttc ttgtgcagct tgatcaatcg
    33301 gctcactggc ttcaggtaag agcgaacgaa tttcgttctg ttccagctca atggttttga
    33361 caattggcgt ggcatcgcgg tgataaacat agggccgcca gacagcgacg gccagagtaa
    33421 gaacggtgag cgaccccaac ataacgcggt gtggtcgcgg taaattatta aacgccaggg
    33481 cgacagagcg ggctatctgt tgcacgtaat cacttcctca ttaatctcct ttcaggcagc
    33541 tcgcatactg gttggctaat tgattcagga attctgaata gcttgtttta cccagtttga
    33601 tattcgtccc caggggatcc aacgttccca tacgaacgga tgtccctcgt gcgacgctct
    33661 caacgaccgc tggcctgaac tgtggctcag caaaaacgca ggttgctttt tgctcaacca
    33721 actgtgttct tatttcatgt aaacgctgcg cgccaggttg aatctcaggg ttaacggtaa
    33781 aatgaccaag cggtgtcagt ccgaactgtt tttcgaagta gccgtaagca tcgtgaaaaa
    33841 cgaaataacc tttccccttg agcggcgcga gctcgttacc aacctgcgtt tcggttgagg
    33901 ctaattgtgc ctcaaaatcc ttcaggttgg cgtcaagttt ggctcgactt tgcggcataa
    33961 gttccactaa ttttccatgg attgcaaccg ctgtagcccg cgctatctct ggggaaagcc
    34021 aaagatgcat gttgaaatcg ccgtgatggt gatcttcgtc acttttttcc gcgtgatcgt
    34081 gatcatcatc atcgtcgtga atacttttca tcagcagcgg tttcacattc tctagctgcg
    34141 caatcgttac ctgtttcgct tctggtaatt tacttaccgg tttttgcata aacgcttcca
    34201 tctccgggcc aacccaaacg actaagtccg cgttctgtaa gcgttttaca tccgatggac
    34261 gcagtgaata atcatgttct gaagcgccgt caggcagtaa aacctgcgtt tctgttaccc
    34321 catcagcaat ggcagaagcg atgaacccaa cgggtttaag cgaagcgaca acggcggcat
    34381 ctgcggcctg tgttgcaccg ccccagagag cggcggataa tgctgcgaaa agaagcgttt
    34441 ttttatgtaa cataatgcga ccaatcatcg taatgaatat gagaagtgtg atattataac
    34501 atttcatgac tactgcaaga ctaaaattaa catgacaagt ctggtttccc tggaaaatgt
    34561 ctcggtttct tttggccaac gccgcgtcct ctctgatgtg tcgctggaac ttaaacctgg
    34621 aaaaattttg actttacttg ggccaaacgg cgcaggtaag tcgacactgg tacgggtagt
    34681 gctcgggctg gtaacacccg atgaaggggt tatcaagcgc aacggaaaac tgcgcatcgg
    34741 ctatgtaccg cagaagctgt atctcgacac cacgttgcca ctgaccgtaa accgtttttt
    34801 acgcttacgc cctggcacac ataaagaaga tattttgcct gcactgaaac gtgtccaggc
    34861 cgggcatctg attaacgcac cgatgcaaaa gctctcgggt ggcgaaacgc agcgtgtact
    34921 gttagcgcga gcattgttaa atcgaccgca attattagtg ctggatgaac ccactcaggg
    34981 cgtggatgtg aatggtcagg tggcgttata tgaccttatt gaccaactgc gtcgcgaact
    35041 ggattgtggc gttttaatgg tatctcacga tctgcatctg gtaatggcaa aaaccgatga
    35101 agtgctttgc ctgaatcacc acatttgttg ttccggcaca ccggaagttg tttccctgca
    35161 tccggagttt atttctatgt ttggtcctcg tggtgctgaa caactgggta tctatcgcca
    35221 tcatcataat catcgtcacg atttacaggg acgaattgtt ttgcgtcggg gaaatgatcg
    35281 ctcatgattg aattattatt tcccggttgg ttagccggga tcatgctcgc ctgtgccgcg
    35341 ggtccgctgg gttcgtttgt agtctggcgt cgtatgtctt atttcggtga tacgctggct
    35401 catgcctcat tacttggcgt cgcgtttggt ttgttgctgg acgtgaatcc attctatgcg
    35461 gtgattgcag ttacgctgct gctggcgggc ggtctggtat ggctggagaa gcgtccacag
    35521 ctggcgatcg acacgttatt agggattatg gcgcacagtg ccctgtcgct gggcctggtg
    35581 gtcgttagtc tgatgtctaa tattcgtgtt gatttgatgg cttacctgtt cggtgattta
    35641 ctggcagtga cgccagaaga tctcatctct attgcgattg gcgtggtcat cgtggtggct
    35701 attttgttct ggcaatggcg caatttgctg tcgatgacga ttagcccgga tctggcgttt
    35761 gttgatggtg tgaaattaca gcgcgtgaaa ttgttgttga tgctggtgac ggcattgacg
    35821 attggtgtag cgatgaaatt tgtcggcgcg ttgattatta cttcactgct gattattcct
    35881 gctgctactg cacgtcgctt tgcccgcacg ccggaacaga tggctggtgt cgctgttttg
    35941 gtggggatgg tggcagtgac tggcggttta accttttccg cattttacga tacacctgca
    36001 ggcccgtcgg tggtgctatg cgcggcactg ttatttattc tcagtatgat gaaaaaacag
    36061 gccagctaat ctttcgctga acatatttgt cggatgcggc gcgagcgcct tatccgacct
    36121 tgtattatcc ctccagtgca gagaaaatcg gccagttttc tctgcctgca gtccgcatgc
    36181 cgtatccgac ctacggttcg ctatctctgg taggcctgat aagacgaaca gcgtcgcatc
    36241 aggcacactg ccagtgtcgg atgcggcgcg agcgaccaat ccgacttacg gcatttctgg
    36301 cggcgttatg ccaaagtgat tccacgcccg cgtcgtcgcc atacgcccac gcggtgtacg
    36361 ctgcaaaaag ccttgctgaa tcaaataagg ttccagtaca tcctcaatgg tttcacgttc
    36421 ttcgccaatg gctgccgcca ggttatccag acctaccggc ccaccaaaga acttatcgat
    36481 taccgccagc aacaatttgc ggtccatata atcgaaacct tcagcatcga cattcaacat
    36541 atccagcgcc tgagcagcga tatctgccga gatggtgcca tcgtgcttca cttcagcgaa
    36601 atcacgcact cgacgcagca gacggttggc aatacgtggc gtaccgcgcg cacgacgagc
    36661 aacttccagc gcgccgtcat cactcatctc aagccccata aagcgtgcgc tgcgactgac
    36721 gatatattgc agatccggca cctgataaaa ctccagacgt tgcacaatac caaaacgatc
    36781 gcgcaacggt gatgtcagcg aacctgcgcg cgtggttgca ccaatcaggg taaacggcgg
    36841 caaatcaatt ttaatggagc gtgccgccgg accttcacca atcatgatat ccagttggta
    36901 atcttccatt gccggataca acacctcttc caccactggt gaaagacggt ggatctcatc
    36961 aataaacagt acatcgtgtg gttcaaggtt agtgagcatt gctgccagat cgcccgcctt
    37021 ttccagcacc ggaccagaag tcgtgcgtaa attaacgccc atttcattgg cgacaatatt
    37081 ggcaagcgta gttttaccca accccggagg accaaaaatc aatagatgat cgagggcatc
    37141 gccgcgcagt ttcgctgctt tgatgaaaat ctccatctgc gaacgaacct gcggctgacc
    37201 aacatactct tccagtaatt tagggcgaat ggcgcgatct gccacatctt ccggcaaagt
    37261 ggtaccggca gaaatcagac ggtctgcttc aatcatcctt tacctcataa cgcggcgcgt
    37321 agggcttcgc gaattaatgt ttcactgctg gcgtcagggc gagcgatttt gctcaccatg
    37381 cggcttgctt cttgtggttt atagcccagt gccaccagcg cagcaaccgc ttcctgttca
    37441 gcatcgtcgg tcgccgggct ggcaggagac gtgagtacca ggtcggcggc tggcgtaaag
    37501 agatcgccat gcaaaccttt aaatcggtct ttcatttcga caatcaagcg ttcggcggtt
    37561 tttttgccaa tacccggcag tttcaccagt gcccccactt cttcacgctc aacggcatta
    37621 acgaactgct gcgctgacat tccggagagg atcgccagcg ccaacttcgg gccgacgccg
    37681 ttggttttga tcaactcttt gaacaacgtg cgctcttgtt tattgttaaa accgtacagc
    37741 agttgcgcgt cttcacgcac cacaaagtgg gtgaaaacga tcgcttcctg acccgcttca
    37801 gggagttcat aaaaacaggt catcggcata tgcacttcat agcctacgcc gcccacttca
    37861 attaacacca gcgggggttg tttttcaatg atgatgcctc tgagtctgcc tatcacatga
    37921 cgctcctgcg taatgaatca aagataatgc tgtatgataa aaaaatgctg gatagatatc
    37981 cagcgaagga tgaagaaaac ttgcgaggtg tctcaatgat ctgaaaaatg gcgcagtata
    38041 atttattcta caggttatat tggaagcaaa tatttaaata ttacatattc agcg
//
LOCUS       DADSYM010000023        37233 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2415
            SAMN08039579-rid11723173.denovo.023, whole genome shotgun sequence.
ACCESSION   DADSYM010000023 DADSYM010000000
VERSION     DADSYM010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039579
            Sequence Read Archive: SRR6362533
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 37233)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37233)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980162.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 03:39:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,484
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 4,347
            CDSs (with protein)               :: 4,347
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 43 of 85
            Pseudo Genes (incomplete)         :: 37 of 85
            Pseudo Genes (internal stop)      :: 19 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37233
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039579-rid11723173.denovo.023"
                     /strain="LGE2415"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8302 N 95.9628 W"
                     /collection_date="2015-08-24"
     gene            64..3822
                     /gene="yfaL"
                     /locus_tag="J1Y83_001012"
     CDS             64..3822
                     /gene="yfaL"
                     /locus_tag="J1Y83_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIDA-I family autotransporter adhesin YfaL/EhaC"
                     /protein_id="HBA7629184.1"
                     /translation="MRIISLRKEYLSLLPSMIASLFSPNGAAAVIDSCQGYDIKASCQ
                     ASRQSLSGNIQDWSIADGQWLIFSGMVNNASGGAVFLQHSAEFTISPQNETGMTLFAD
                     NSVSGEYNNGGAIFAKENSTINLANVIFDSNVAGGYGGAIYSAGTNDTGAADLRVTNA
                     VFHNNIANDGKGGAIYTINNDVYLSDDAFNNNQAYTSTSYSDGDGGAIDVTDNSTDNT
                     HLSGKTIINNTSFTNNYAEGYGGAIYTSSTTSPYLIDISVDDNYDQNNGVMIDENNSA
                     SGYDHSATAAAGGFMYIGHSVAEFNIAADKTLVIGNTSNDGAIDSLAGTGVIVKEGAG
                     ELVLNADNNAFTGEMSIQNGEVTLGRSDELMNVGDTHCQSDPQDCFGLMVGSTVHSEY
                     QAELNVGNTQQTFVHSLTGFANGILNIDAGGNVTVNQGGFSGSIQGEGQLTVAQDGSY
                     LLTGAQSMALTGDIVVEDNAVLSLAGNQADLRAMQSDPQSIVLNGGVLDLSDFTTWDG
                     DSSYNDGLQISGSGGTVIGSNDVVDISSGDDLHIGGSDASQNGVYVVINAGDQRVTLA
                     NNNGYLGNTQIASGTLEVSDNSQLGDTSYNRSVIFTDPQQHSEMDVTTDVDTRSATTG
                     QGRNIEMRADGEIHVEDGVDTQWGGLMADSTGQQLDSVSTLTKSGGGTLELTASGTAT
                     SAVRVEDGTLKGEAENIIPYVSSLWVGEDGVFETGQNQDIRSIDATSGGDIDITDGTV
                     LRLTQQDTNQALDASLFSGDGTLVNATDGVTLAGELNTNLETDSLTYLSDVTVNGNLT
                     NTSGAISLQNSVAGDTLTVNGDYTGGGTLFLDSELNGDDSASDQLVLNGNTSGNTTVV
                     VNPITGIGEPTSTGIKVVDFAADPAQFQNNAQFSLAGSGYVNMGAYDYTLVEDNNDWY
                     LRSQEVNPTPPPDPDPTPDPDPTPDPDPTPDPEPTPAYQPVLNAKVGGYFNNLRAANQ
                     AFVMERRDHAGGDGQTLNLRVIGGRYHYTAVGQLAQHEDTSTVQLSGNLFSGHWGDDG
                     EWMLGIVGGYSDNQGDSRSNMTGTRADNQNHGYAVGLTSSWYQHGNQKQGAWLDSWLQ
                     YVWFNNDVSEQDDGVDHYHSSGIIASLEAGYQWLPGHGVVIEPQTQVIYQGVQQDDFT
                     AANHARVSQSQGDDIQTRLGLHSEWRTAVGVTPTLDLNYYHDPHATEIEEDGSTISDD
                     AVKQRGEIKVGITGNISQRVSLRGSVAWQKGSDDFAQTAGFLSMTVKW"
     gene            complement(3962..4684)
                     /locus_tag="J1Y83_001013"
     CDS             complement(3962..4684)
                     /locus_tag="J1Y83_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-demethylubiquinone
                     3-O-methyltransferase/2-octaprenyl-6-hydroxy phenol
                     methylase"
                     /protein_id="HBA7629185.1"
                     /translation="MNAEKSPVNHNVDHEEIAKFEAVASRWWDLEGEFKPLHRINPLR
                     LGYIAERAGGLFGKKVLDVGCGGGILAESMAREGATVTGLDMGFEPLQVAKLHALESG
                     IQVDYVQETVEEHAAKHAGQYDVVTCMEMLEHVPDPQSVVRACAQLVKPGGDVFFSTL
                     NRNGKSWLMAVVGAEYILRMVPKGTHDVKKFIKPAELLGWVDQTSLKERHMTGLHYNP
                     ITNTFKLGPGVDVNYMLHTQNK"
     gene            4831..7458
                     /gene="gyrA"
                     /locus_tag="J1Y83_001014"
     CDS             4831..7458
                     /gene="gyrA"
                     /locus_tag="J1Y83_001014"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit A"
                     /protein_id="HBA7629186.1"
                     /translation="MSDLAREITPVNIEEELKSSYLDYAMSVIVGRALPDVRDGLKPV
                     HRRVLYAMNVLGNDWNKAYKKSARVVGDVIGKYHPHGDSAVYDTIVRMAQPFSLRYML
                     VDGQGNFGSIDGDSAAAMRYTEIRLAKIAHELMADLEKETVDFVDNYDGTEKIPDVMP
                     TKIPNLLVNGSSGIAVGMATNIPPHNLTEVINGCLAYIDDEDISIEGLMEHIPGPDFP
                     TAAIINGRRGIEEAYRTGRGKVYIRARAEVEVDAKTGRETIIVHEIPYQVNKARLIEK
                     IAELVKEKRVEGISALRDESDKDGMRIVIEVKRDAVGEVVLNNLYSQTQLQVSFGINM
                     VALHHGQPKIMNLKDIIAAFVRHRREVVTRRTIFELRKARDRAHILEALAVALANIDP
                     IIELIRHAPTPAEAKTALVANPWQLGNVAAMLERAGDDAARPEWLEPEFGVRDGLYYL
                     TEQQAQAILDLRLQKLTGLEHEKLLDEYKELLDQIAELLRILGSADRLMEVIREELEL
                     VREQFGDKRRTEITANSADINLEDLITQEDVVVTLSHQGYVKYQPLSEYEAQRRGGKG
                     KSAARIKEEDFIDRLLVANTHDHILCFSSRGRVYSMKVYQLPEATRGARGRPIVNLLP
                     LEQDERITAILPVTEFEEGVKVFMATANGTVKKTVLTEFNRLRTAGKVAIKLVEGDEL
                     IGVDLTSGEDEVMLFSAEGKVVRFKESSVRAMGCNTTGVRGIRLGEGDKVVSLIVPRG
                     DGAILTATQNGYGKRTAVAEYPTKSRATKGVISIKVTERNGLVVGAVQVDDCDQIMMI
                     TDAGTLVRTRVSEISIVGRNTQGVILIRTSEDENVVGLQRVAEPVDEEDLDTIDGSAA
                     EGDDEIAPEVDVDDEPEEE"
     gene            7607..9295
                     /locus_tag="J1Y83_001015"
     CDS             7607..9295
                     /locus_tag="J1Y83_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416733.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2138 domain-containing protein"
                     /protein_id="HBA7629187.1"
                     /translation="MSGEKKAKGWRFYGLVGFGAIALLSAGVWALQYSGSGPEKTLSP
                     LVVHNNLQIDLNEPDLFLDSDSLSQLPKDLLTIPFLHDVLSEDFVFYYQNHADRLGIE
                     GSIRRIVYEHDLTLKDKLFSSLLDQPAQAALWHDKQGHLSHYMVLIQRSGLSKLLEPL
                     LFAATSDSQLSKTEISSIKINSETIPVYQLRYNGNNVLMFATYQDKILAFSSSDMLFK
                     DDQQDTEATAIASDLLSGKKRWETRFGLEARTAEKTPVRQRIVVSARLLGFGYQRLMP
                     SFAGVRFEMGNDGWHSFLALNDESASVDASFDFTPVWNSMPAGASFCVAVPYSHGIAE
                     EMLSHISQENDKLNGALDGGAGLCWYEDSKLQTPLFVGQFDGTAEQAQLPGKLFTQNI
                     GAHESKAPEGVLPVSQTQQGEAQIWRREVSSRYGQYPKAQAAQPDQLMSDYFFRVSLA
                     MQNKTLLFSLDDTLVNNALQALNKTRPAMVDVIPTDGIVPLYINPQGMAKLLRNETLT
                     SLPKNLEPVFYNAAQTLLMPKLDALSQQPRYVMKLAQMEPGAAWQWLPITWQPL"
     gene            9292..9915
                     /locus_tag="J1Y83_001016"
     CDS             9292..9915
                     /locus_tag="J1Y83_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311139.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1175 domain-containing protein"
                     /protein_id="HBA7629188.1"
                     /translation="MRHGLLVLICWLYCVVAHSEMLNVEQSGLFRAWFVRIAQEQLRQ
                     GPSPRWYQQDCAGLVRFAANEALKVHDSKWLKSNGLSNQYLPPEMTLTPEQRQLAQNW
                     NQGNGKTGPYVTAINLIQYNSQFIGQDINQALPGDMIFFDQGDAQHLMVWMGRYIIYH
                     TGSATKTDNGMRAVSLQQLMTWKDTRWIPNDSNPNFIGIYRLNFLAR"
     gene            10059..14453
                     /locus_tag="J1Y83_001017"
     CDS             10059..14453
                     /locus_tag="J1Y83_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311138.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-2-macroglobulin family protein"
                     /protein_id="HBA7629189.1"
                     /translation="MRLEAPGRDYRRYQMEEYGGVDVRLYRIPDPMAFLRQQKNLHRI
                     VVQPQYLGDGLNNTLTWLWDNWYGKSRRVMQRTFSSQSRQNVTQALPELQLGNAIIKP
                     SRYVQNSQFSPLKKYPLVEQFRYPLWQATSFEPQQGVKLEGASSNFISPQPGNIYIPL
                     GKQEPGLYLVEAMVGGYRATTVVFVSDTVALSKVSGNELLVWTAGKKQGEAKPGSEIL
                     WTDGLGVMTRGVTDGSGTLQLQHISPERSYILGKDAEGGVFVSENFFYESEIYNTRLY
                     IFTDRPLYRAGDRVDVKMMGREFHDPLHSSPIVSAPAKLSVLDANGSLLQTVNVTLDA
                     RNGGQGSFRLPENAVAGGYELRLAYRKQVYSSSFRVANYIKPHFEIGLALDKKEFKTG
                     EAVSGKLQLLYPDGEPVKDARVQLSLRAQQLSMVGNDLRYAGRFPVSMEGSEKVSDDN
                     GHVALNLPAADKPSRYLLTVSASDGAAYRVTTTKEILIERGLAHYSLSTAAQYSNSGE
                     SVVFRYAALESSKQVPVTYEWLRLEDRTSHSGELQSGGKSFTVNFAKPGNYNLTLRDK
                     DGLILAGLSHAVSGKGSTSHSGTVDIVVDKTLYQPGETAKMLITFPEPIDEALLTLER
                     DRVEQQSLLSHPANWLTLQRLNDTQYEARVPVSNSFAPNITFSVLYTRNGQYSFQNAG
                     IKVAVPQLDIRVKTDKTHYQPGELVNVELTSSLKGKPVSAQLTVGVVDEMIYALQPEI
                     APNIGKFFYPLGRNNVRTSSSLSFISYDQALSSEPVAPGATNRSERRVKMLERPRREE
                     VDTAAWMPSLTTDKQGKAYFTFLMPDSLTRWRITARGMNGDGLVGQGRAYLRSEKSLY
                     MKWSMPTVYRMGDKPAAGLFIFSQQDNEPVALVTKFAGAEMRQTLTLHKGANYISLTQ
                     NIQQSGLLSAELQQNGQVQDSISTKLSFVDNSWPVEQQKNVMLGGGENALMLPEQASN
                     IRLQSSETPQEIFRNNLDALVDEPWGGVINTGSRLIPLSLAWRSLADHQSAAANDIRQ
                     MIQDNRLRLMQLAGPGARFTWWGEDGNGDAFLTAWAWYADWQASQALGVTQQPEYWQH
                     MLDSYAEQADNMPLLHRALVLAWAQEMNLPCKTLLKGLDEAIARRGTKTKDFSEADTS
                     DINDSLILDTPESPLADAVANVLTMTLLKKAQLKSTVMPQVQQYAWDKAANSNQPLAH
                     TVVLLNSGGDATQAAAILSGLTAEQSTIERALAMNWLAKYMATMPPVVLPAPAGAWAK
                     HKLTGGGEYWRWVGQGVPDILSFGDELSPQNVQVRWREPAKTAQQSNIPVTVERQLYR
                     LIPGEEEMSFTLQPVTSNEIDSDALYLDEITLTSEQDAVLRYGQVEVPLPPGADVERT
                     TWGISVNKPNAGKQQGQLLEKARNEMGELAYMVPVKELTGTVTFRHLLRFSQKGQFVL
                     PPARYVRSYAPAQQSVAAGSEWTGMQVK"
     gene            14454..16103
                     /locus_tag="J1Y83_001018"
     CDS             14454..16103
                     /locus_tag="J1Y83_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2300 domain-containing protein"
                     /protein_id="HBA7629190.1"
                     /translation="MNWRRIVWLLALVTLPTLAEETPLQLVLRGAQHDQLYQMSSSGV
                     TKVSALPDTLTTPLGSLWKLYVYAWLEDTHQPEQPYQCRGNSPEEVYCCQAGESITRD
                     TALVRSCGLYFAPQRLHIGADMWGQYWQQRQAPAWLASLPTLKPETSVTVKSLLDSLA
                     TLPAQNKAQEVLLDVVLDEAKIGVASMLGSRVRVKTWSWFADDKQQIRQGGFAGWLTD
                     GTPLWVTGSGTSKTVLTRYATVLNRVLPVPTQVASGQCVEVELFARYPLKKVTEEKST
                     TAVKPGVLNGRYRVTFANGNHMTFVSHGETTLLTVKGKLKLQSHLDREEYVARVLDRE
                     AKSTPPEAAKAMTVAIRTYLQQNADRDGDCLSIPDSSATQRVSASPATVGARTMTAWT
                     QDLIYAGDPVHYHGSRVTEGTLSWRHATAQAGQGERYDQILAFAYPDNNLSRWGAPRS
                     TCQLLPKAKAWLAKKMPQWRRILQGETGYNEPDVFAVCRLVSGFPYTDRQQKRLFIRN
                     FFTLQDRLDLTHEYLHLAFDGYPTGLDENYIETLTRQLLMD"
     gene            16108..16884
                     /locus_tag="J1Y83_001019"
     CDS             16108..16884
                     /locus_tag="J1Y83_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfaP family protein"
                     /protein_id="HBA7629191.1"
                     /translation="MRKIFLPLLLVALSPVAHSEGVQEVEIDAPLSGWHPAEGEDASF
                     SQTINYPASSVNMADDQNISAQIRGKIKNYAAAGKVQQGRLVVNGASMPQRIESDGSF
                     ARPYIFTEGSNSVQVISPDGQSRQKMQFYSTPGAGAIRARLRLVLSWDTDNTDLDLHV
                     VTPDGEHAWYGNTVLKNSGALDMDVTTGYGPEIFAMPAPVHGRYQVYINYYGGRSETE
                     LTTAQLTLITDEGSVNEKQETFIVPMRNAGELTLVKSFDW"
     gene            complement(16958..18142)
                     /locus_tag="J1Y83_001020"
     CDS             complement(16958..18142)
                     /locus_tag="J1Y83_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HBA7629192.1"
                     /translation="MKNCVIVSAVRTAIGSFNGSLASTSAIDLGATVIKAAIERAKID
                     SLHVDEVIMGNVLQAGLGQNPARQALLKSGLAETVCGFTVNKVCGSGLKSVALAAQAI
                     QAGQAQSIVAGGMENMSLAPYLLDAKARSGYRLGDGQVYDVILRDGLMCATHGYHMGI
                     TAENVAKEYGITREMQDELALHSQRKAAAAIESGAFTAEIVPVNIVTRKKTFVFSQDE
                     FPKADSTAEALGALRPAFDKAGTVTAGNASGINDGAAALVIMEESSALAAGLTPLARI
                     KSYASGGVPPALMGMGPVPATQKALQLAGLQLADIDLIEANEAFAAQFLAVGKTLGFE
                     PEKVNVNGGAIALGHPIGASGARILVTLLHAMQARDKTLGLATLCIGGGQGIAMVIER
                     LN"
     gene            complement(18173..19495)
                     /locus_tag="J1Y83_001021"
     CDS             complement(18173..19495)
                     /locus_tag="J1Y83_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00366 family protein"
                     /protein_id="HBA7629193.1"
                     /translation="MIGRISRFMTRFVSRWLPDPLIFAMLLTLLTFVIALWLTPQTPI
                     SMVKMWGDGFWNLLAFGMQMALIIVTGHALASSAPVKSLLRTAASAAKTPVQGVMLVT
                     FFGSVACVINWGFGLVVGAMFAREVARRVPGSDYPLLIACAYIGFLTWGGGFSGSMPL
                     LAATPGNPVEHIAGLIPVGDTLFSGFNIFITVALIVVMPFITRMMMPKPSDVVSIDPK
                     LLMEEADFQKQLPKDAPPSERLEESRILTLIIGALGIAYLAMYFSEHGFNITINTVNL
                     MFMIAGLLLHKTPMAYMRAISAAARSTAGILVQFPFYAGIQLMMEHSGLGGLITEFFI
                     NVANKDTFPVMTFFSSALINFAVPSGGGHWVIQGPFVMPAAQALGADLGKSVMAIAYG
                     EQWMNMAQPFWALPALAIAGLGVRDIMGYCITALLFSGVIFVIGLTLF"
     gene            complement(19492..20142)
                     /locus_tag="J1Y83_001022"
     CDS             complement(19492..20142)
                     /locus_tag="J1Y83_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase subunit B"
                     /protein_id="HBA7629194.1"
                     /translation="MDAKQRIARRVAQELRDGDIVNLGIGLPTMVANYLPEGIHITLQ
                     SENGFLGLGPVTTAHPDLVNAGGQPCGILPGAAMFDSAMSFALIRGGHIDACVLGGLQ
                     VDEEANLANWVVPGKMVPGMGGAMDLVTGSRKVIIAMEHCAKDGSAKILRRCTMPLTA
                     QHAVHMLVTELAVFRFIDGKMWLTEIADGGDLATVRAKTEARFEVAADLNTQRGDL"
     gene            complement(20142..20804)
                     /gene="atoD"
                     /locus_tag="J1Y83_001023"
     CDS             complement(20142..20804)
                     /gene="atoD"
                     /locus_tag="J1Y83_001023"
                     /EC_number="2.8.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate CoA-transferase subunit alpha"
                     /protein_id="HBA7629195.1"
                     /translation="MKTKLMTLQDATGFFRDGMTIMVGGFMGIGTPSRLVEALLESGV
                     RDLTLIANDTAFVDTGIGPLIVNGRVRKVIASHIGTNPETGRRMISGEMDVVLVPQGT
                     LIEQIRCGGAGLGGFLTPTGVGTVVEEGKQTLTLDGKTWLLERPLRADLALIRAHRCD
                     TLGNLTYHLSARNFNPLIALAADITLVEPDELVETGELQPDHIVTPGAVIDHIIVSQE
                     SK"
     gene            complement(21000..22385)
                     /gene="atoC"
                     /locus_tag="J1Y83_001024"
     CDS             complement(21000..22385)
                     /gene="atoC"
                     /locus_tag="J1Y83_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetoacetate metabolism transcriptional
                     regulator AtoC"
                     /protein_id="HBA7629196.1"
                     /translation="MTAINRILIVDDEDNVRRMLSTAFALQGFETHCANNGRTALHLF
                     ADIHPDVVLMDIRMPEMDGIKALKEMRSHETRTPVILMTAYAEVETAVEALRCGAFDY
                     VIKPFDLDELNLIVQRALQLQSMKKEIRHLHQALSTSWQWGHILTNSPAMMDICKDTA
                     KIALSQASVLISGESGTGKELIARAIHYNSRRAKGPFIKVNCAALPESLLESELFGHE
                     KGAFTGAQTLRQGLFERANEGTLLLDEIGEMPLVLQAKLLRILQEREFERIGGHQTIK
                     VDIRIIAATNRDLQAMVKEGTFREDLFYRLNVIHLILPPLRDRREDISLLANHFLQKF
                     SSENQRDIIDIDPMAMSLLTAWSWPGNIRELSNVIERAVVMNSGPIIFSEDLPPQIRQ
                     PVCNAGEAKTAPVGERNLKEEIKRVEKRIIMEVLEQQEGNRTRTALMLGISRRALMYK
                     LQEYGIDPADV"
     gene            complement(22382..24208)
                     /gene="atoS"
                     /locus_tag="J1Y83_001025"
     CDS             complement(22382..24208)
                     /gene="atoS"
                     /locus_tag="J1Y83_001025"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     AtoS"
                     /protein_id="HBA7629197.1"
                     /translation="MHYMKWIYPRRLRNQMILMAILMVIVPTLTIGYIVETEGRSAVL
                     SEKEKKLSAVVNLLNQALGDRYDLYIDLPREERIRALNAELAPITENITHAFPGIGAG
                     YYNKTLDAIITYAPSALYQNNVGVTIAADHPGREVMRTNTPLVYSGRQVRGDILNSMI
                     PIERNGEILGYIWANELTEDIRRQAWKMDVRIIIVLTAGLLISLLLIVLFSRRLSANI
                     DIITDGLSTLAQNIPTRLPQLPGEMGQISQSVNNLAQALRETRTLNDLIIENAADGVI
                     AIDRQGDVTTMNPAAEVITGYQRHELVGQPYSMLFDNTQFYSPVLDTLEHGTEHVALE
                     ISFPGRDRTIELSVTTSRIHNTHGEMIGALVIFSDLTARKETQRRMAQAERLATLGEL
                     MAGVAHEVRNPLTAIRGYVQILRQQTSDLIHQEYLSVVLKEIDSINKVIQQLLEFSRP
                     RHSQWQQVSLNALVEETLVLVQTAGVQARVDFISELDNELSPINADRELLKQVLLNIL
                     INAVQAISARGKIRIRTWQYSDSQQAISIEDNGSGIDLSLQKKIFDPFFTTKASGTGL
                     GLALSQRIINAHQGDIRVASLPGYGATFTLILPINPQGNQTV"
     gene            24375..27224
                     /gene="rcsC"
                     /locus_tag="J1Y83_001026"
     CDS             24375..27224
                     /gene="rcsC"
                     /locus_tag="J1Y83_001026"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     RcsC"
                     /protein_id="HBA7629198.1"
                     /translation="MKYLASFRTTLKASRYMFRALALVLWLLIAFSSVFYIVNALHQR
                     ESEIRQEFNLSSDQAQRFIQRTSDVMKELKYIAENRLSAENGVLSPRGRETQTDVPAF
                     EPLFADSDCSAMSNTWRGSLESLAWFMRYWRDNFSAAYDLNRVFLIGSDNLCMANFGL
                     RDMPVERDTALKALHERINKYRNAPQDDSGSNLYWISEGPRPGVGYFYALTPVYLANR
                     LQALLGVEQTIRMENFFLPGTLPMGVTILDENGHTLISLTGPESKIKGDPRWMQERSW
                     FGYTEGFRELVLKKNLPPSSLSIVYSVPVDKVLERIRMLILNAILLNVLAGAALFTLA
                     RMYERRIFIPAESDALRLEEHEQFNRKIVASAPVGICILRTADGVNILSNELAHTYLN
                     MLTHEDRQRLTQIICGQQVNFVDVLTSNNTNLQISFVHSRYRNENVAICVLVDVSSRV
                     KMEESLQEMAQAAEQASQSKSMFLATVSHELRTPLYGIIGNLDLLQTKELPKGVDRLV
                     TAMNNSSSLLLKIISDILDFSKIESEQLKIEPREFSPREVMNHITANYLPLVVRKQLG
                     LYCFIEPGVPVALNGDPMRLQQVISNLLSNAIKFTDTGCIVLHVRADGDYLSIRVRDT
                     GVGIPAKEVVRLFDPFFQVGTGVQRNFQGTGLGLAICEKLISMMDGDISVDSEPGMGS
                     QFTVRIPLYGAQYPQKKGVEGLSGKRCWLAVRNASLCQFLETSLQRSGIVVTTYEGQE
                     PTPEDVLITDEVVNKKWQGRAVVTFCRRHIGIPLEKAPGEWVHSVAAPHELPALLARI
                     YLIEMESDDPANALPSTDKAVSDNDDMMILVVDDHPINRRLLADQLGSLGYQCKTAND
                     GVDALNVLNKNHIDIVLSDVNMPNMDGYRLTQRIRQLGLTLPVIGVTANALAEEKQRC
                     LESGMDSCLSKPVTLDVIKQTLTVYAERVRKSRES"
     gene            complement(27310..27960)
                     /gene="rcsB"
                     /locus_tag="J1Y83_001027"
     CDS             complement(27310..27960)
                     /gene="rcsB"
                     /locus_tag="J1Y83_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator RcsB"
                     /protein_id="HBA7629199.1"
                     /translation="MNNMNVIIADDHPIVLFGIRKSLEQIEWVNVVGEFEDSTALINN
                     LPKLDAHVLITDLSMPGDKYGDGITLIKYIKRHFPSLSIIVLTMNNNPAILSAVLDLD
                     IEGIVLKQGAPTDLPKALAALQKGKKFTPESVSRLLEKISAGGYGDKRLSPKESEVLR
                     LFAEGFLVTEIAKKLNRSIKTISSQKKSAMMKLGVENDIALLNYLSSVTLSPADKD"
     gene            complement(27977..30649)
                     /gene="rcsD"
                     /locus_tag="J1Y83_001028"
     CDS             complement(27977..30649)
                     /gene="rcsD"
                     /locus_tag="J1Y83_001028"
                     /EC_number="2.7.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase RcsD"
                     /protein_id="HBA7629200.1"
                     /translation="MRQKETTATTRFSLLPGSITRFFLLLIIVLLVTMGVMVQSAVNA
                     WLKDKSYQIVDITHAIQKRVDTWRYVTWQIYDNIAATTSPSSGEGLQETRLKQDVYYL
                     EKPRRKTEALIFGSHDNSTLEMTQRMSTYLDTLWGAENVPWSMYYLNGQDNSLVLIST
                     LPLKDLTSGFKESTISDIVDSRRAEMLQQANALDERESFSNMRRLAWQNGHYFTLRTT
                     FNQPGHLATVVAFDLPINDLIPPGMPLDSFRLEPDATATGNNENEKEGTDSVSIHFNS
                     TKIEISSALNSTDMRLVWQVPYGTLLLDTLQNILLPLLLNIGLLALALFGYTTFRHFS
                     SRSTESVPSTAVNNELRILRAINEEIVSLLPLGLLVHDQESNRTVISNKIADHLLPHL
                     NLQNITTMAEQHQGIIQATINNELYEIRMFRSQVAPRTQIFIIRDQDREVLVNKKLKQ
                     AQRLYEKNQQGRMTFMKNIGDALKEPAQSLAESAAKLNAPEGKQLANQADVLVRLVDE
                     IQLANMLADDSWKSETELFSVQDLIDEVVPSVLPAIKRKGLQLLINNHLKAHDMRRGD
                     RDALRRILLLLMQYAVTSTQLGKITLEVDQDESSEDRLTFRILDTGEGVSIHEMDNLH
                     FPFINQTQNDRYGKADPLAFWLSDQLARKLGGHLNIKTRDGLGTRYSVHIKMLAADPE
                     VEEEEERLLDDVCVMVDVTSAEIRNIVTRQLENWGATCITPDERLISQDYDIFLTDNP
                     SNLTASGLLLSDDESGVREIGPGQLCVNFNMSNAMQEAVLQLIEVQLAQEEVTESPLG
                     GDENAQLHASGYYALFVDTVPDDVKRLYTEAATSDFAALAQTAHRLKGVFAMLNLVPG
                     KQLCETLEHLIREKDVPGIEKYISDIDSYVKSLL"
     gene            31388..32491
                     /gene="ompC"
                     /locus_tag="J1Y83_001029"
     CDS             31388..32491
                     /gene="ompC"
                     /locus_tag="J1Y83_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpC"
                     /protein_id="HBA7629201.1"
                     /translation="MKVKVLSLLVPALLVAGAANAAEVYNKDGNKLDLYGKVDGLHYF
                     SDDKSVDGDQTYMRLGFKGETQVTDQLTGYGQWEYQIQGNSAENENNSWTRVAFAGLK
                     FQDVGSFDYGRNYGVVYDVTSWTDVLPEFGGDTYGSDNFMQQRGNGFATYRNTDFFGL
                     VDGLNFAVQYQGKNGSVSGEGMTNNGRDALRQNGDGVGGSITYDYEGFGIGGAISSSK
                     RTDAQNTAAYIGNGDRAETYTGGLKYDANNIYLAAQYTQTYNATRVGSLGWANKAQNF
                     EAVAQYQFDFGLRPSLAYLQSKGKNLGTIGTRNYDDEDILKYVDVGATYYFNKNMSTY
                     VDYKINLLDDNQFTRDAGINTDNIVALGLVYQF"
     gene            32603..33658
                     /gene="apbE"
                     /locus_tag="J1Y83_001030"
     CDS             32603..33658
                     /gene="apbE"
                     /locus_tag="J1Y83_001030"
                     /EC_number="2.7.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase ApbE"
                     /protein_id="HBA7629202.1"
                     /translation="MEISFTRVALLAAALFFVGCDQKPQPAKTHATEVTVLEGKTMGT
                     FWRASIPGIDAKRSAELKEKIQTQLDADDQLLSTYKKDSALMRFNDSQSLSPWPVSEA
                     MADIVTTSLRIGAKTDGAMDITVGPLVNLWGFGPEQQPVQIPSQEQIDAMKAKTGLQH
                     LTVINQSHQQYLQKDLPDLYVDLSTVGEGYAADHLARLMEQEGISRYLVSVGGALNSR
                     GMNGAGKPWRVAIQKPTDKENAVQAVVDINGHGISTSGSYRNYYELDGKRLSHVIDPQ
                     TGRPIEHNLVSVTVIAPTALEADAWDTGLMVLGPEKAKEVVRREGLAVYMITKEGDNF
                     KTWMSPQFKSFLISEKN"
     gene            33732..34796
                     /gene="ada"
                     /locus_tag="J1Y83_001031"
     CDS             33732..34796
                     /gene="ada"
                     /locus_tag="J1Y83_001031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-binding transcriptional
                     regulator/O6-methylguanine-DNA methyltransferase Ada"
                     /protein_id="HBA7629203.1"
                     /translation="MKNATCLTDDQRWQSVLARDPNADGEFVFAVRTTGIFCRPSCRA
                     RHALRENVSFYANASEALAAGFRPCKRCQPDKANPRQHRLDKITHACRLLEQETPVTL
                     EALADQVAMSPFHLHRLFKATTGMTPKAWQQAWRARRLRELLAKGESVTTSILNAGFP
                     DSSSYYRKADETLGMTAKQFRHGGENLAVRYALADCELGRCLVAESERGICAILLGDD
                     DATLISELQQMFPAADSAPADLTFQQHVREVIASLNQRDTPLTLPLDIRGTAFQQQVW
                     QALRTIPCGETVSYQQLANAIGKPKAVRAVASACAANKLAIVIPCHRVVRGDGTLSGY
                     RWGVSRKAQLLRREAENEER"
     gene            34796..35447
                     /gene="alkB"
                     /locus_tag="J1Y83_001032"
                     /pseudo
     CDS             34796..35447
                     /gene="alkB"
                     /locus_tag="J1Y83_001032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311128.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA oxidative demethylase AlkB"
     gene            35523..37166
                     /gene="yojI"
                     /locus_tag="J1Y83_001033"
     CDS             35523..37166
                     /gene="yojI"
                     /locus_tag="J1Y83_001033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin J25 efflux ABC transporter YojI"
                     /protein_id="HBA7629204.1"
                     /translation="MELLVLVWRQYRWPFISVMALSLASAALGIGLIAFINQRLIETA
                     DTSLLVLPEFLGLLLLLMAVTLGSQLALTTLGHHFVYRLRSEFIKRILDTHVERIEQL
                     GSASLLAGLTSDVRNITIAFVRLPELVQGIILTIGSAAYLWMLSGKMLLVTAIWMAVT
                     IWGGFVLVARVYKHMATLRETEDKLYTDFQTVLEGRKELTLNRERAEYVFNNLYIPDA
                     QEYRHHIIRADTFHLSAVNWSNIMMLGAIGLVFWMANSLGWADTNVAATYSLTLLFLR
                     TPLLSAVGALPTLLTAQVAFNKLNKFALAPFKAEFPRPQAFPNWQTLELRNVTFSYQD
                     NAFSVGPINLTIKRGELLFLIGGNGSGKSTLAMLLTGLYQPQNGEILLDGKPVSGEQP
                     EDYRKLFSAVFTDVWLFDQLLGPEGQPANPQLVEKWLVQLKIAHKLELSNGRIVNLKL
                     SKGQKKRVALLLALAEERDIILLDEWAADQDPHFRREFYQVLLPLMQEMGKTIFAISH
                     DDHYFIHADRLLEMRNGQLSELTGEERDAASRDAVARTA"
BASE COUNT         9105 a         9140 c         9994 g         8994 t
ORIGIN      
        1 ataccctata tcgcataatg cgattaaata cgccatctta tagaaaatga tggttagatg
       61 aatatgcgga ttatctctct acgcaaggag tatttatctt tactcccgtc aatgattgca
      121 tctcttttct ctcctaacgg tgccgcagcg gtcattgatt catgccaggg atatgatatc
      181 aaagcgagct gtcaggccag caggcaaagc ctttcgggta atatacagga ctggagcatc
      241 gcggatggac aatggttgat tttttcgggt atggtcaata atgccagcgg tggcgcagta
      301 tttttgcagc acagtgctga atttacgata tcaccacaaa atgaaacagg gatgaccctg
      361 tttgctgata actcggttag tggcgaatat aataatggtg gggctatctt tgctaaagaa
      421 aactcaacga taaatcttgc gaatgttatt ttcgacagta atgttgcagg aggctatggc
      481 ggagcaatct attctgccgg taccaatgat accggtgccg ccgatttacg tgtcactaac
      541 gccgtatttc acaataacat cgctaatgac ggcaaaggcg gtgcaattta taccatcaat
      601 aatgacgtct atttaagtga tgatgctttt aataataacc aggcatatac atcaacaagt
      661 tatagtgatg gggatggcgg cgctattgat gttacggata atagtacgga taacacacat
      721 ctttcaggaa aaacgataat taataatacc tcctttacca ataactatgc agaaggttat
      781 ggcggagcaa tttataccag cagcaccaca tccccgtatc ttattgatat ttctgttgat
      841 gataattatg accaaaacaa tggtgtgatg attgatgaaa ataacagcgc ctcgggatat
      901 gaccattcgg cgacggcagc ggcggggggc tttatgtata tcggtcatag tgtggctgaa
      961 tttaacattg ccgccgataa aacgctggta ataggcaaca ccagcaacga tggtgcgatt
     1021 gattctcttg ccggaacggg agtcatcgtg aaagagggag cgggagagtt agtcctcaat
     1081 gccgacaata atgcgtttac cggcgagatg agtatccaga atggcgaggt gacgctgggg
     1141 cgcagcgatg agttaatgaa tgtcggcgac acgcactgtc agagcgatcc gcaggattgc
     1201 tttggtctga tggtcggcag cactgttcat tctgagtatc aggcagaact gaatgttggc
     1261 aatacacaac aaacgtttgt gcactcatta accggttttg ctaatggcat tttgaatatc
     1321 gacgcaggcg gcaatgttac agtaaaccag ggcggttttt ccggctcgat tcagggtgaa
     1381 ggacagttga cggtagcgca agatggcagc tatctcctga caggggcgca gtcgatggca
     1441 ttaaccggcg atattgtggt ggaagataac gccgtattgt cgctggcggg gaatcaggcc
     1501 gatttacggg caatgcagag cgatccgcaa tcgatcgtgc taaatggcgg agtattggat
     1561 ctctctgatt tcaccacctg ggatggcgat agctcataca atgacggtct gcaaatcagc
     1621 ggcagcgggg ggacagtgat tggcagtaat gatgtcgttg atatcagcag tggcgatgat
     1681 ttacatattg gcggcagcga tgccagccag aatggcgttt atgtcgtcat taatgcgggg
     1741 gatcagcgcg taacgctggc gaataacaac ggttatctcg gtaatacgca aatcgcctcc
     1801 ggtacgcttg aagtcagcga taattcgcaa ttgggggaca caagttacaa ccgttcagtg
     1861 atcttcacag atccccaaca acacagtgaa atggatgtga cgactgatgt tgacactcgc
     1921 tcggctacaa caggtcaggg caggaatatt gaaatgcgcg ctgatggcga aatacacgtt
     1981 gaggatggcg tagatacgca atggggcggg ttgatggcgg atagtaccgg gcaacaactg
     2041 gatagcgtaa gcacgttgac taaaagtggc ggcggtacgc tggagttgac cgccagcggc
     2101 actgcgacgt ctgcggtacg tgtggaggac ggaacgctaa aaggtgaagc ggagaatatt
     2161 attccttatg tttcttcact gtgggtgggg gaagacggtg tttttgaaac cgggcaaaat
     2221 caggatattc gttcaatcga tgccacctct ggcggcgata tcgacataac cgatggtact
     2281 gtgttgcgat tgacgcagca ggatacgaac caggcgctgg atgcctcgct gtttagcggc
     2341 gacggtacgc tggtgaatgc caccgatggc gtgacgctgg caggtgagct taataccaac
     2401 cttgaaactg acagcctgac ttatctttcc gacgtgacag ttaatggcaa tctgaccaat
     2461 acgtccggtg cgattagcct gcaaaatagc gtcgctggcg acacgctgac ggtaaacggt
     2521 gattacaccg gtggcggtac gttgtttctc gacagcgaat taaacggcga tgactcggca
     2581 agcgaccaac tggtgttgaa cggcaatacc tcgggtaaca cgaccgtggt ggttaacccc
     2641 attactggca ttggtgaacc gacatctaca ggtattaaag tagttgattt cgcagctgat
     2701 cccgcgcagt ttcaaaacaa tgcgcagttc agtctggcag gaagcggcta cgtcaatatg
     2761 ggggcgtatg actacacgct ggtggaagat aacaacgact ggtatctgcg atcacaagaa
     2821 gtcaatccga cgccgccgcc tgatccagac ccgactccag atcctgatcc cacgccggat
     2881 cccgacccaa cccccgaccc ggaacctacg cctgcctacc agccggtgtt gaatgccaaa
     2941 gttggtggat attttaataa cctgcgggcg gcaaatcagg catttgtgat ggagcgacgc
     3001 gatcacgctg gtggcgatgg tcagacgctg aatttacgcg ttatcggcgg gcgttatcat
     3061 tacacggcgg tagggcagtt ggctcagcat gaagacactt ctacggtgca gcttagcggc
     3121 aacctgttta gcgggcactg gggtgatgat ggcgagtgga tgcttgggat tgttggtggc
     3181 tacagcgata accagggcga cagccgctcg aatatgaccg gaactcgcgc cgataaccag
     3241 aaccacggtt atgccgttgg gctgacctca agctggtatc agcacggtaa tcagaagcag
     3301 ggggcctggt tggatagctg gctgcaatat gtgtggttta acaatgatgt ttccgaacaa
     3361 gacgatggcg tggatcatta ccactcgtcg gggattatcg cctcgctgga agccggttat
     3421 cagtggttac cggggcatgg tgtggtgatt gaaccgcaga cgcaggtgat ttatcagggc
     3481 gtgcagcagg atgattttac cgccgctaac catgcgcgcg tgtcacaatc gcagggtgat
     3541 gatattcaga cgcggctggg tttacacagc gaatggcgta ctgctgttgg tgtcacgcca
     3601 acgttagatc tgaattatta tcacgatccc catgcgacgg aaattgaaga agatggcagc
     3661 actatcagtg acgatgcggt gaagcaacgg ggtgaaataa aagtgggaat aacgggcaat
     3721 atcagtcagc gagtttcgct gcgcggcagc gtggcgtggc agaaagggag tgatgatttt
     3781 gcccagacgg cagggttttt gtcgatgaca gtgaaatggt aatcgctgtc tgaccggcac
     3841 tgttgccgga tgcggcgtaa acgccttatc tggcctacgg ggagcatttg taggcctgat
     3901 aagacgcggc aagcgtcgca tcaggcttgg cgcggttgcc ggatgaatcc aaacaacaac
     3961 ctcacttatt ctgcgtgtgc agcatatagt tcacatccac gccggggccg agcttaaagg
     4021 tgttagtgat agggttgtaa tgcagcccgg tcatatgccg ttctttaaga ctggtctgat
     4081 ccacccagcc cagcaattct gccggtttaa taaacttctt cacatcatgt gtacctttag
     4141 gcaccatgcg caaaatatat tccgcaccaa ccaccgccat cagccatgac ttgccgttgc
     4201 ggttaagcgt cgagaaaaag acatcgccgc ccggtttcac cagttgcgca caggctctga
     4261 ccactgactg aggatcgggg acgtgctcca gcatctccat acaggtcacc acgtcatact
     4321 gtccggcatg ttttgccgcg tgctcttcca cggtttcctg cacgtaatcc acctgaatgc
     4381 cgctttccag tgcgtgcagt tttgccacct gcaatggctc aaagcccata tccagaccgg
     4441 tcaccgtcgc gccctcccgc gccatactct cggccagaat gccgccgcca caaccgacat
     4501 cgagcacctt tttgccaaat aaaccgccag cacgctcggc aatatagccc agacgcagcg
     4561 ggttaatgcg gtgcagcggt ttgaactcac cttcaagatc ccaccagcgg gaggcgacgg
     4621 cttcaaattt agcgatctct tcgtggtcta cgttgtggtt taccggcgat ttttcggcat
     4681 tcattggcac ttctactccg taattggcaa gacaaacgag tatatcaggc attggatgtg
     4741 aataaagcgt ataggtttac ctcaaactgc gcggctgtgt tataatttgc gacctttgaa
     4801 tccgggatac agtagaggga tagcggttag atgagcgacc ttgcgagaga aattacaccg
     4861 gtcaacattg aggaagagct gaagagctcc tatctggatt atgcgatgtc ggtcattgtt
     4921 ggccgtgcgc tgccagatgt ccgagatggc ctgaagccgg tacaccgtcg cgtactttac
     4981 gccatgaacg tactaggcaa tgactggaac aaagcctata aaaaatctgc ccgtgtcgtt
     5041 ggtgacgtaa tcggtaaata ccatccccat ggtgactcgg cggtttatga cacgatcgtc
     5101 cgtatggcgc agccattctc gctgcgttac atgctggtag acggtcaggg taacttcggt
     5161 tccatcgacg gcgactctgc ggcggcaatg cgttatacgg aaatccgtct ggcgaaaatt
     5221 gcccatgaac tgatggccga tctcgaaaaa gagacggtcg atttcgttga taactatgac
     5281 ggtacggaaa aaattccgga cgtcatgcca accaaaattc ctaacctgct ggtgaacggt
     5341 tcttccggta tcgccgtagg tatggcaacc aacatcccgc cgcacaacct gacggaagtc
     5401 atcaacggtt gtctggcgta tatcgatgat gaagacatca gcattgaagg gctgatggaa
     5461 cacatcccgg ggccggactt cccgacggcg gcaatcatta acggtcgtcg cggtattgaa
     5521 gaagcttacc gtaccggtcg cggcaaggta tatatccgcg cccgtgccga agtagaagtt
     5581 gacgccaaaa ccggacgtga aaccattatc gtccacgaaa ttccttatca ggtgaacaaa
     5641 gcgcgcctga tcgagaagat tgcggaactg gtaaaagaaa aacgcgtgga aggcatcagc
     5701 gcgctgcgtg acgagtctga caaagacggt atgcgcatcg tgattgaagt gaaacgcgat
     5761 gcggtcggtg aagttgtgct taacaacctc tactcccaga cccagttgca ggtttctttc
     5821 ggtatcaaca tggtggcatt gcaccatggt cagccgaaga tcatgaacct gaaagacatc
     5881 atcgcggcgt ttgttcgtca ccgccgtgaa gtagtgaccc gtcgtactat tttcgaactg
     5941 cgtaaagctc gcgatcgtgc tcatatcctt gaagcattag ccgtggcgct ggcgaacatc
     6001 gacccgatca tcgaactgat ccgtcatgcg ccgacgcctg cagaagcgaa aaccgcgctg
     6061 gttgcaaatc cgtggcagct gggcaacgtt gctgcgatgc tggaacgtgc gggcgacgat
     6121 gctgcgcgtc cggaatggct ggagccagag ttcggcgtgc gtgatggtct gtactacctg
     6181 accgaacagc aagctcaggc gattctggat ctgcgtctgc agaaactgac cggccttgag
     6241 cacgaaaaac tgctcgacga atacaaagag ctgctggatc agattgcgga actgttgcgt
     6301 attcttggta gcgccgatcg tctgatggaa gtgatccgtg aagagctgga gttggttcgt
     6361 gaacagttcg gtgacaaacg tcgtaccgaa atcaccgcta acagcgccga catcaacctg
     6421 gaagatctga tcacccagga agatgtggtg gtcacgcttt ctcaccaggg ttacgtgaag
     6481 tatcagccgc tttctgaata cgaagcgcag cgtcgtggcg ggaaaggtaa atctgccgca
     6541 cgtattaaag aagaagactt tatcgaccga ctgctggtgg cgaacactca cgaccatatt
     6601 ctgtgcttct ccagccgtgg ccgcgtctat tcgatgaagg tctaccagtt accagaagct
     6661 actcgtggcg cacgtggtcg tccgatcgtc aatctgctgc cgctggaaca ggacgaacgt
     6721 atcaccgcga tcctgccggt gaccgagttt gaagaaggcg tgaaagtctt catggcgacc
     6781 gctaacggta ctgtgaagaa aaccgtcctc accgagttca accgtctgcg taccgccggt
     6841 aaagtggcga tcaaactggt tgaaggtgat gagctgatcg gcgttgacct gaccagcggc
     6901 gaagacgaag taatgctgtt ctccgctgaa ggtaaagtgg tgcgctttaa agagtcttct
     6961 gtccgtgcga tgggctgcaa caccaccggt gttcgtggta ttcgtttagg cgaaggcgat
     7021 aaagtcgtct ctctgatcgt gcctcgtggc gatggcgcaa tcctcaccgc aacgcagaac
     7081 ggttatggta aacgtaccgc agtggcggaa tacccaacca agtcgcgtgc gacgaaaggg
     7141 gttatctcca tcaaggttac cgaacgtaac ggtttagtgg ttggcgcggt gcaggtagat
     7201 gactgcgacc agatcatgat gatcaccgat gccggtacgc tggtacgtac tcgcgtttcg
     7261 gaaatcagca tcgtaggccg taacacccag ggcgtaatcc ttatccgtac ttcggaagat
     7321 gaaaacgtgg tgggtctgca acgtgttgct gaaccggttg acgaggaaga tctggatacc
     7381 atcgacggca gtgccgcgga aggggacgat gaaatcgctc cggaagtgga cgttgacgac
     7441 gagccagaag aagaataatt tcatcgcttc atgccaaaag ggagccatct cccttttttg
     7501 aattgaaaag tccaggctgt aaagtctggg cttttgtcgt attagggcgc ggtaaagttt
     7561 ggctgtgccc gtaaaaaatg gctggctata cacaaggaat gtggcaatga gtggtgaaaa
     7621 aaaggcgaaa ggctggcggt tttatggcct tgttggtttt ggggcaatag cgctgctatc
     7681 cgctggcgta tgggcattac aatactctgg cagtgggcca gaaaaaacgt tgtcgccact
     7741 ggtggtgcac aacaatctgc aaatcgatct caatgagccg gacctctttc tcgacagcga
     7801 ctctctgagc cagcttccca aagatctcct taccattccg tttctccacg atgttctgag
     7861 cgaagatttc gttttctatt atcagaatca tgccgatcgt ctgggcattg aaggcagcat
     7921 tcgccgcatt gtctatgaac acgatctcac gctgaaagat aagctctttt cgtcactctt
     7981 agaccagccc gcgcaggcgg cactgtggca tgataaacaa ggtcatcttt cacattatat
     8041 ggtgcttatc cagcgcagtg gtttaagcaa actgctggag ccattgttgt ttgccgctac
     8101 cagcgacagc cagttaagca agacggaaat cagtagcatc aagataaata gtgaaactat
     8161 tcctgtttat cagttgcgct ataacggcaa taacgtcctg atgtttgcga cttatcagga
     8221 caaaatattg gcgttttcca gttcggatat gctgtttaaa gatgaccaac aggataccga
     8281 agctacggcg atcgcaagtg atttgttgag tggtaaaaaa cgctgggaaa cccgctttgg
     8341 cctggaagcg cgtactgccg aaaaaacgcc agtacgccag cgcatcgtag tcagcgccag
     8401 gttgctgggg tttggctacc agcgattaat gccttctttt gctggcgtgc gcttcgaaat
     8461 gggtaacgac ggctggcaca gttttctggc gttaaacgat gaatccgcca gcgtagatgc
     8521 cagtttcgat tttacgccgg tatggaacag tatgcctgcc ggagccagct tctgtgtggc
     8581 ggtgccgtat tcacacggta ttgccgaaga gatgctttcg cacatcagcc aggaaaacga
     8641 caagctgaat ggggcgttag acggtggcgc ggggctgtgc tggtatgaag actcaaaatt
     8701 gcaaacccca ctgtttgttg gtcagtttga tggcactgcc gaacaggcgc aattgccagg
     8761 gaaactgttt acgcaaaata ttggtgcgca cgaaagcaaa gcgccagaag gtgttttgcc
     8821 ggtaagccag actcagcagg gcgaagcgca aatctggcgt cgcgaagtga gttcccgata
     8881 cggtcagtat ccgaaagcgc aggcggcgca acccgatcaa ttaatgtcgg attatttttt
     8941 ccgcgtgtcg ctggcgatgc aaaacaaaac gctgcttttc tccctcgatg acacgctggt
     9001 taataacgcc ctgcaagcac tgaataaaac ccgcccggca atggtggatg taatacccac
     9061 tgatggcatc gttccgctct atatcaatcc acaaggaatg gcgaaactgc tgcgtaacga
     9121 aacgctgacc agtctgccga agaatctcga accggttttt tataacgccg cacaaacttt
     9181 attaatgccg aagctggacg ctttatctca acaaccgcgt tatgtcatga agctggccca
     9241 gatggaaccc ggtgccgcct ggcagtggct acccataacc tggcaaccgc tatgagacac
     9301 gggctgctgg tgctgatttg ctggctgtat tgtgttgttg cccatagtga aatgctaaat
     9361 gtcgaacaat ccgggctgtt tcgcgcgtgg tttgtgcgca ttgcacagga acagctccgc
     9421 caggggccaa gcccacgctg gtatcaacag gattgcgcgg gcctggtgcg atttgcggcg
     9481 aacgaggcgc tgaaagttca cgacagtaaa tggctaaaaa gtaacggttt atctaaccag
     9541 tatttgccgc cagagatgac gctaacacct gaacagcgtc aactggcgca aaactggaat
     9601 caggggaatg ggaaaaccgg cccctatgtg accgcgatta atttgattca gtacaacagc
     9661 cagtttattg gccaggacat aaaccaggca ctgcctggcg atatgatttt tttcgatcag
     9721 ggcgatgccc agcacttaat ggtctggatg gggcgttaca tcatctacca caccggaagc
     9781 gccacgaaaa ctgacaacgg aatgcgcgca gtcagtctgc aacaacttat gacatggaag
     9841 gacacccgat ggatacccaa cgattccaat cccaatttca ttggcattta tcgtttaaat
     9901 tttctggcgc gatagcagcg tgcttgtccc tgtctcttgt gggaacaggg cttgctaatg
     9961 ctgatgattc gcttccttcc agtaactatg cgccgcctgc cgggggaaca ttctttttgc
    10021 ttgctgacag cagttttagc agcagtgaag aggcgaaagt gcgactggaa gcgccggggc
    10081 gtgattatcg gcgctatcag atggaagagt acggcggcgt ggacgttcgc ctgtatcgta
    10141 ttcctgaccc gatggcattt ttgcgccagc agaaaaacct gcatcgcatt gtggtgcaac
    10201 cgcaatatct gggcgacggg ctaaacaata cgctgacctg gctgtgggat aactggtacg
    10261 gcaaatctcg ccgcgtgatg cagcgtactt tctcttctca gtcacggcag aatgtgactc
    10321 aggcgttacc cgaattacag ctcggcaatg ccattattaa accttcccgt tatgtacaga
    10381 acagccagtt ttccccgctg aaaaaatatc cactggtgga acagttccgt tacccattat
    10441 ggcaggctac atcgttcgag ccgcagcaag gggtaaaact ggaaggcgca tccagcaatt
    10501 tcatttcgcc gcagccgggc aacatctata ttcctctcgg caaacaagag ccgggactgt
    10561 acctcgtcga ggcgatggtg ggtgggtatc gggcgacgac ggtggtgttt gtttccgata
    10621 ccgtggcgct tagcaaagtg tcaggtaacg agcttctggt gtggaccgcg gggaaaaaac
    10681 agggtgaagc gaagcccggc tcagagatct tgtggactga cggtcttggc gtgatgaccc
    10741 gcggtgtgac cgatggcagt ggtaccttgc agttacaaca tatatcgcca gaacgttcat
    10801 atattctggg taaggatgct gaaggcggcg tttttgtctc cgagaacttt ttctacgaaa
    10861 gcgaaatcta caacacccgc ttgtatatct ttaccgatcg cccgctatat cgcgcaggcg
    10921 atcgtgtcga tgttaaaatg atgggccgcg agttccacga tccgttgcat tcatccccca
    10981 tcgtcagcgc accggcgaag ctttcggtgc tggacgccaa cggcagtctg ttgcaaaccg
    11041 tcaatgtcac gctggatgcg cgtaatggcg ggcaggggag tttccgcctg ccagaaaatg
    11101 ccgtcgccgg aggttatgag ttacgtcttg cttaccgcaa acaggtctat agcagcagtt
    11161 ttcgcgtggc aaactacatc aaaccccatt ttgagattgg tttagctctc gacaaaaaag
    11221 agttcaaaac aggcgaagcg gtcagcggca aactgcaact gctctacccg gatggcgagc
    11281 cggtaaaaga tgcccgcgtg cagttaagtt tgcgcgctca gcaattatca atggtcggta
    11341 acgatttgcg ttatgccgga cgtttcccgg tgtcgatgga aggcagtgaa aaggtttccg
    11401 acgataatgg tcatgtggcg ttgaatctcc ccgccgccga taaaccgagc cgttatttgt
    11461 taaccgtctc cgccagtgac ggcgcggcgt atcgagttac caccaccaaa gagatcctca
    11521 ttgaacgtgg cctggcgcat tactctttaa gtaccgcagc acaatacagt aatagcggcg
    11581 agtcggttgt gttccgttat gccgcgctgg aatcttcaaa acaggttcct gttacgtatg
    11641 aatggttgcg tctcgaagac cgcacgagcc acagcggaga gctacagtca ggcggcaaat
    11701 cctttaccgt caatttcgct aaacctggca attacaacct gacgttacgc gataaagacg
    11761 gcttaattct cgccgggtta agccatgcag tcagcggtaa gggcagcacg tcgcatagtg
    11821 gtacggtaga tatcgtggtg gataaaacgc tgtaccagcc tggcgaaacc gcgaagatgc
    11881 tgattacctt cccggagcca attgacgaag cattattgac gctggaacgc gatcgcgtag
    11941 aacagcagtc gctgctatcg catccggcaa actggctaac gctacaacgt ttaaacgata
    12001 cccagtatga agcccgcgta ccggtgagca attcctttgc gcctaacatc actttttcgg
    12061 tgctgtatac ccgtaacggt cagtacagtt ttcagaacgc cgggattaaa gttgccgttc
    12121 cccagctgga tatccgggtg aaaacggaca aaacccatta ccagcctggt gaactggtca
    12181 atgtcgaatt aacctcgtcg ctgaaaggta aacctgtttc tgcgcagtta acggtaggcg
    12241 tggtcgatga aatgatctac gcgctgcaac cggaaatcgc gccgaatatc ggcaaatttt
    12301 tctatccgct ggggcgtaac aatgtgcgta ccagctccag tctgtcgttt atcagctacg
    12361 atcaggcgct ctccagcgag ccggttgcgc ctggcgcgac taaccgcagc gagcggcgag
    12421 taaaaatgct ggaacgtcca cggcgtgaag aggtggatac cgcggcctgg atgccgtcac
    12481 tcacaaccga taaacaaggc aaagcgtatt tcacgttcct gatgcctgat tcgttaaccc
    12541 gctggcgcat caccgcgcgt gggatgaacg gcgacgggct ggtcgggcag gggcgtgctt
    12601 atctgcgttc ggaaaaaagt ctctacatga aatggagtat gccaacggtg tatcgcatgg
    12661 gcgacaaacc ggcggcagga ctgtttatct tcagtcagca ggataacgaa ccggtggcgc
    12721 tggtgactaa atttgcaggt gctgagatgc gccagacgct gacgctgcac aaaggggcga
    12781 attatatttc gctgacgcag aatattcagc aatctggctt gttaagtgca gaactgcaac
    12841 aaaatgggca agtgcaggac agcattagca caaaactgtc ttttgtggac aacagctggc
    12901 ccgttgaaca gcagaaaaat gtcatgctcg gcggtggcga gaacgcgctg atgttgcccg
    12961 agcaggcgag caatatccgg cttcaaagta gtgaaacgcc gcaggagatt ttccgcaaca
    13021 atcttgatgc gttagtcgat gaaccgtggg gtggggtgat caacaccggt agccgtctga
    13081 tcccgctcag tctcgcctgg cgttcgcttg ccgatcatca aagtgccgcc gctaacgaca
    13141 ttcgtcagat gattcaggat aaccgtctgc ggctgatgca actggcaggg cccggtgcgc
    13201 gctttacctg gtggggtgaa gatggcaatg gtgacgcctt cctcacggca tgggcatggt
    13261 acgccgactg gcaggccagc caggcgctcg gcgtaacgca gcaaccggaa tactggcagc
    13321 atatgctcga cagctacgcg gagcaggcgg ataacatgcc gttattgcat cgggcgctgg
    13381 tgctggcgtg ggcgcaggag atgaatctgc cgtgcaaaac gttgttgaaa gggttggatg
    13441 aagctatcgc ccggcgcgga actaaaacta aagatttctc tgaggcagac accagcgata
    13501 tcaatgacag tctgatcctc gatacacctg aatctccact ggcagatgcg gtggcaaacg
    13561 tcttaaccat gacgttgctg aaaaaagcgc agttgaagtc cactgtgatg ccacaggttc
    13621 agcaatatgc gtgggataaa gcggcaaaca gcaatcagcc gctggcgcac acggttgtgc
    13681 tgctcaatag cgggggcgac gctacccagg cggctgctat tttaagtggt ttgaccgctg
    13741 agcaatctac tattgagcgc gcgctggcca tgaactggct ggcgaaatat atggcgacaa
    13801 tgcctccagt tgttttgcct gcgcctgcgg gcgcatgggc caaacataag ttaactggag
    13861 ggggcgaata ctggcgttgg gttggtcagg gcgtgccgga cattctctct tttggtgacg
    13921 aattatcgcc gcaaaatgtg caggtccgct ggcgtgaacc ggcaaaaacg gctcaacaaa
    13981 gtaacattcc ggtgaccgtt gaacgccagt tgtatcggct tatccccggt gaagaagaga
    14041 tgagctttac tctgcagccg gtgaccagca atgagattga cagcgatgcg ctgtatctcg
    14101 atgaaattac gcttaccagc gagcaggatg ccgttctgcg ctatggtcag gtggaagtac
    14161 cgctcccgcc gggagccgac gttgagcgca caacatgggg catttcggtc aataaaccta
    14221 acgctggaaa acagcagggg caattgctgg aaaaagcgcg taatgaaatg ggcgaactgg
    14281 cctatatggt gccggtgaaa gaactgacgg gaacggtcac tttccgccat ttgctgcgct
    14341 tctcgcaaaa agggcaattc gttctgcctc ctgctcgtta tgtgcgttcc tatgcacctg
    14401 cacagcaaag tgttgcggca gggagtgaat ggaccgggat gcaggtgaaa taagtgaact
    14461 ggcgcagaat cgtctggcta ttggcgttgg ttactctgcc aacgcttgcg gaggagacgc
    14521 cgttacaact ggtgctgcgc ggtgcgcagc acgatcaact gtatcagatg tcgtcatcag
    14581 gcgtgaccaa agtctcagca ttacctgaca cgctcaccac gccgttaggg agtttatgga
    14641 aactgtatgt ttacgcctgg ctggaggata cccatcaacc ggagcagccc taccagtgtc
    14701 gcggaaactc cccggaagag gtttattgtt gtcaggctgg ggagagcata acgcgcgata
    14761 ccgcgctggt acgctcctgt ggactctatt ttgcgccgca acggttgcat attggtgcag
    14821 atatgtgggg gcaatactgg caacagcgtc aggcacctgc gtggttagcg tctcttccga
    14881 cgctgaaacc agaaacctcg gtaacggtta aatcgctgct tgattcatta gcgacgttac
    14941 ccgcgcaaaa caaagctcag gaagtcttac tggatgtggt gctggacgag gcgaaaatcg
    15001 gtgtcgcatc catgctcggt agtcgggtac gggtgaaaac ctggagctgg tttgccgatg
    15061 ataaacagca aatccgtcag ggtggttttg ccggctggtt aaccgatggt acaccgctgt
    15121 gggttactgg cagtggcacc agcaaaacgg tactgacccg ttatgcgacg gtgcttaatc
    15181 gggtattgcc agtgccgacg caagttgcca gtgggcaatg tgtagaggtg gagctgtttg
    15241 ctcgttatcc gctgaagaaa gttactgagg aaaagagtac gacggcagtc aaaccaggcg
    15301 tgctcaatgg gcgctatcgt gtgacgtttg ccaatggtaa ccacatgaca tttgtcagcc
    15361 acggtgagac aacgttactg acggtaaagg gcaaactgaa attacagtcg catctcgatc
    15421 gtgaagagta cgttgccaga gttctggatc gcgaggcgaa aagtacgcct cctgaggcag
    15481 cgaaagccat gaccgtggcg attcgcacgt atttacagca aaacgccgat cgcgatggtg
    15541 attgcctgag catcccggac agtagcgcta cccagcgcgt ttcagcttcg ccagccactg
    15601 taggcgcgcg gacgatgacc gcctggacgc aggatctcat ctatgcgggc gatccggttc
    15661 attatcatgg cagccgggtt accgaaggga cgctttcctg gcggcacgct actgcccagg
    15721 cggggcaggg agagcgttac gatcagatac ttgcgtttgc ttatcctgac aacaacctta
    15781 gccgctgggg agcgccacgc tcaacctgcc agttattacc caaagcgaaa gcctggctgg
    15841 cgaaaaaaat gccgcagtgg cggcgtatat tacaaggtga gacgggatac aacgaaccag
    15901 acgtgtttgc ggtctgtcgt ttagtctcag gtttccccta taccgatcgg cagcagaaac
    15961 ggctgtttat tcgtaacttc tttacgcttc aggatcggct ggatttaacc catgagtatc
    16021 tgcacctggc ctttgatggt tatcccaccg ggcttgatga gaactatatc gaaacgctga
    16081 cccgacaatt attaatggac taatgctatg cgaaagattt ttcttccgtt gttactggtg
    16141 gcgctttcgc ctgtcgctca cagtgaaggt gtgcaggaag tcgagattga tgcaccgctc
    16201 tctggctggc atccggcaga gggtgaagat gccagttttt cacaaacgat caattacccg
    16261 gcgtcgtccg tcaacatggc agatgatcaa aatatctcag cgcagatccg cggcaaaata
    16321 aagaattatg ctgcggcggg taaagttcag cagggccggc tggtggtcaa cggtgccagt
    16381 atgccgcaga gaattgaatc agatggttct tttgcacgcc cttatatttt cactgaaggc
    16441 agcaacagcg tgcaggtcat cagcccagat gggcaaagcc gacaaaaaat gcagttttac
    16501 tcaacgccgg gtgctggggc gattcgcgca cgtttacggc tggttctctc gtgggatacg
    16561 gacaataccg acctcgatct tcatgtcgtt acgcctgacg gtgaacatgc ctggtacggt
    16621 aacaccgtgc tgaaaaacag tggtgcactg gatatggatg tcacgacagg gtacggcccc
    16681 gagattttcg ccatgccagc gccagttcac ggccgttatc aggtgtatat caactactat
    16741 ggcggacgca gcgaaacgga attaactacg gcccaactga cgctgatcac cgatgaaggg
    16801 tcggtcaatg agaaacagga aacttttatt gtaccgatgc gtaatgctgg cgaactgacg
    16861 ctggtgaaaa gttttgactg gtgattaatg aggttatctt tactgtcaat gttcaagaaa
    16921 acgcccgatg actttcgcta tcgggcgttt ttacatgtta attcaatcgt tcaatcacca
    16981 tcgcaattcc ctggccgcca ccaatgcaca gtgttgccag ccccagcgtt ttatcgcgtg
    17041 cctgcattgc atgtaatagt gtgaccagaa tacgagcacc actggcaccg ataggatgcc
    17101 ccagcgcgat ggccccgccg ttgacattca ctttctcagg ctcaaagccc agggttttcc
    17161 caacggcaag gaactgtgca gcaaatgctt cattagcctc aatgagatca atatccgcca
    17221 gttgcagccc cgccagttgt aacgcttttt gcgtggcagg tactggcccc atacccatca
    17281 atgcgggggg cacgccaccg ctggcataac ttttaatgcg agccaggggg gtaaggcctg
    17341 ctgccagcgc cgaagattct tccataatca ccagagcggc agcaccgtcg ttaatgccag
    17401 acgcgttccc ggcggtgact gttcctgctt tatcgaaggc cgggcgcaat gcgcctaacg
    17461 cttcagccgt agaatccgct ttcgggaatt cgtcttgact gaagacgaag gttttcttcc
    17521 gggtgacaat atttaccggg acgatttcgg ctgtaaaagc accggactca attgcggcag
    17581 ccgctttacg ctgtgaatgt agcgccagtt catcctgcat ttcacgggta attccgtact
    17641 ctttagccac gttttcggcg gtaatcccca tatgataacc atgggtggcg cacatcagac
    17701 catcgcgcag gattacgtca taaacctgtc cgtctccaag acgataacca gagcgtgctt
    17761 ttgcatcgag taagtagggc gctaaactca tattttccat accccccgcc acaatgctct
    17821 gcgcctgacc tgcctgaatt gcctgggcgg caagcgccac acttttaaga cccgaaccac
    17881 acactttatt gaccgtgaat ccgcacactg tttcggccag tccgcttttt aacagtgcct
    17941 gacgcgccgg attttgcccc agcccggctt gtagcacgtt acccataatc acttcatcaa
    18001 cgtgtagtga atcgattttt gcacgttcaa tggcggcttt aattactgtc gcccccaggt
    18061 cgatggcgct ggtggaagcg agtgaaccgt taaaactacc gatagcagta cgtaccgcac
    18121 tgacgatgac acaatttttc attttatatt ccttctgtct gtagggttgc cgtcagaaca
    18181 gcgttaaacc aatgacgaaa atgacgccgg agaagagcag ggcagtgatg caatagccca
    18241 tgatgtcgcg gacaccgagt ccggcgattg ccagtgctgg cagcgcccag aacggttgtg
    18301 ccatgttcat ccattgctcg ccgtaggcga tcgccattac cgatttaccg agatcagcgc
    18361 ccagcgcctg ggctgcgggc atcacgaacg gcccctgaat aacccagtgc ccgccgccag
    18421 acggaacggc gaagttaatc agtgcagaac taaaaaaggt catgaccggg aaggtgtctt
    18481 tgttcgcaac attgatgaag aattcggtaa tgagtccgcc cagaccggag tgctccatca
    18541 tcagttggat ccccgcgtag aaggggaatt gcaccagaat accggcggta ctgcgtgctg
    18601 ccgcgctgat agcacgcata taagccattg gcgttttatg tagcagcaga cccgcaatca
    18661 taaacatcag gttgacggta ttgatggtga tgttgaagcc atgttcgctg aagtacatcg
    18721 ccaggtaagc gataccgagt gcgccgatga tcaacgtcag aatacggctt tcttccagtc
    18781 gctcggacgg tggagcatct ttcggtagtt gcttttgaaa atcagcctct tccatgagta
    18841 gtttcggatc gatactcacc acgtcagacg gttttggcat catcatgcgg gtgataaatg
    18901 gcatcaccac aatcaacgcc acagtgatga aaatattaaa accactgaac agagtatcgc
    18961 ccaccgggat cagcccggcg atatgctcaa ccgggttgcc cggtgttgca gccaacagag
    19021 gcattgagcc agagaaaccg ccaccccagg tgagaaaacc aatgtaggcg caggcaatga
    19081 gcaacggata atcagaaccg ggtactcgcc gggcgacttc acgggcgaac attgcaccga
    19141 caaccaaacc aaatccccag ttgatgacac aagcaactga accgaagaaa gtaaccaaca
    19201 tgacgccctg tacgggcgtc tttgcggcgg aggcggcagt acgcagcaaa cttttcaccg
    19261 gagcggagct ggcaagggca tgaccggtaa cgatgataag cgccatctgc ataccaaacg
    19321 ccagcaagtt ccagaaaccg tcaccccaca ttttcaccat gctgatcggc gtttgtggtg
    19381 ttaaccaaag cgcgatcacg aatgttagca atgtcagcaa catggcaaag atcagtggat
    19441 cgggaagcca ccggctgaca aaacgcgtca taaaacgcga tatgcgacca atcataaatc
    19501 accccgttgc gtattcagat cggcagcgac ttcaaaccga gcttctgttt tggcacgcac
    19561 ggtggctaaa tcacccccgt cggcaatttc ggtgagccac attttgccgt caataaaacg
    19621 aaagacagcc agttcagtaa ccagcatatg caccgcatgt tgcgcagtga gtggcatggt
    19681 gcagcggcgc aaaatttttg ctgaaccatc tttggcgcaa tgttccatgg cgatgatcac
    19741 tttgcgcgac ccggtcacca gatccatcgc gccacccata ccgggcacca ttttcccagg
    19801 cactacccag ttcgcgaggt ttgcttcttc gtctacttgc aaaccgccga gcacgcaggc
    19861 atcaatatga ccgccacgga ttagcgcaaa tgacatggcg ctatcaaaca tggctgcacc
    19921 gggtaaaatg ccgcacggtt gtccgccagc gttcaccaga tcgggatgcg ctgttgtgac
    19981 cgggcctaaa ccgaggaagc cgttttccga ttgcagagtg atatgaatac cctccggtaa
    20041 ataattggcg accattgtgg gtaaaccgat tcctaagtta acgatgtcac catcacgaag
    20101 ctcttgcgcc acacggcgcg caatacgttg tttcgcatcc attatttgct ctcctgtgaa
    20161 acgatgatgt ggtcgataac ggcaccaggt gtgacaatat ggtcaggttg cagctcgccg
    20221 gtttcgacca gttcatccgg ctctaccagc gtgatatcag ccgcaagggc tatcaggggg
    20281 ttaaagttgc gggcgctaag gtgataggtc aggttgccaa gtgtgtcgca acgatgagcg
    20341 cgaattagcg ccaggtcggc gcgcagcggg cgttcgagca gccaggtttt accgtcgagt
    20401 gtcagtgtct gtttgccttc ctctacgacg gtgccgacac cagttggggt gagaaaacca
    20461 ccaagtccag ctccgccaca gcgaatttgc tcgattagcg taccttgcgg caccagaacg
    20521 acgtccatct caccagatat catgcgccga cctgtttccg ggttggtgcc gatatgtgaa
    20581 gcaatcactt tgcggactcg accattgacg atgagcggac cgatgcctgt atcaacaaac
    20641 gcggtatcat tggctatcaa tgtcaggtcg cgaacaccag attccagtaa tgcttcaacc
    20701 aggcgggatg gagtgccaat ccccataaat ccgcccacca tgatggtcat gccgtcacga
    20761 aagaagccgg tggcgtcttg taatgtcatc aattttgttt tcatttttta tccctcttgc
    20821 atacaccgtt gtgggtacag tcagggaata gcaaggggag tgccagaatg agattattga
    20881 atgttgttat ttaactaatt gaatagaaaa ggataaatta gatggggatt cataaggaga
    20941 atgagcggct atgcagaaaa ttgcgcactg tgcaaatttc tgcatagcaa gttttggcgt
    21001 tatacatccg ccggatcgat accgtattcc tggagtttat acatcaatgc acggcgactg
    21061 atgcccagca ttaaagcagt gcgggttcgg tttccttctt gttgttccag cacttccata
    21121 atgatgcgtt tttcgacgcg tttaatttcc tcttttaaat tacgctcacc gacaggggct
    21181 gtttttgctt caccagcatt acagactggc tgacgaatct gcggcggaag atcctcagaa
    21241 aaaatgatcg ggcctgaatt catcacgacg gcgcgttcaa taacgttgga aagctctcga
    21301 atatttcccg gccatgacca ggcggtaagc agtgacattg ccatcggatc gatgtcgata
    21361 atatcgcgct gattctcact actgaatttt tgcaaaaagt gattagctaa cagggaaata
    21421 tcttcccggc gatcgcgcag aggcggcagt attaaatgaa taacgttaag gcgataaaag
    21481 agatcttcac ggaaggtgcc ttctttcacc attgcctgca agtcgcggtt ggtggcagca
    21541 atgatgcgga tatcaacttt tatggtctga tggccgccaa tccgttcaaa ttcccgttcc
    21601 tgtagaatgc gtagtaattt ggcttgcagt accagcggca tttcgccaat ttcgtcgagg
    21661 agcagagtac cttcgttggc acgttcaaat aatccctgac gcaaggtttg tgcaccagta
    21721 aatgcacctt tttcatgacc aaacagttca ctttcgagca acgattccgg cagcgccgcg
    21781 cagttgactt taatgaacgg cccctttgcc cgccgcgaat tgtagtgaat cgctctggca
    21841 atcaactctt tcccggtgcc gctttcaccg ctaatcaaga cgctggcctg agaaagggca
    21901 attttggcgg tgtctttgca gatgtccatc atcgccgggc tgttggtgag aatgtgcccc
    21961 cattgccagc tggtgctcag tgcctggtgc agatgacgga tctctttttt cattgactgg
    22021 agttgtaaag cgcgctgaac gattaaattc aactcatcga gatcaaacgg tttgataaca
    22081 tagtcgaagg ctccgcagcg tagtgcttcg acggcggttt ccacttcagc ataggccgtc
    22141 atcagaataa cgggtgtccg ggtctcatgg ctgcgcatct cctttagtgc cttgatgccg
    22201 tccatctctg gcatgcggat atccatcaac accacgtcag ggtgaatatc ggcaaacagg
    22261 tgtaatgctg tgcgcccgtt gttcgcacaa tgtgtttcga atccttgtag tgcaaaagcg
    22321 gtgctcagca tacggcgaac attatcttca tcatccacaa taaggatgcg attaatagca
    22381 gtcatacagt ctgatttccc tgcgggttga tcggtaaaat aagcgtgaag gttgcgccgt
    22441 agcccggcaa actggcgacg cgaatatcac cctgatgggc attaatgatg cgttgactta
    22501 acgccagacc aagcccggtt cctgaggctt tggtagtgaa aaagggatcg aagatctttt
    22561 tttgcagcga gagatcaatg ccactgccgt tgtcctctat cgaaatggcc tgttgcgagt
    22621 cgctatactg ccaggttcga atgcgaattt tccctcgtgc actgatagcc tggacggcat
    22681 tgatcaggat attcagtagt acctgtttga gcagttcacg atcggcgtta atcgggctta
    22741 attcattatc cagttcgctt atgaagtcga cccgcgcttg tacgccggcg gtttgtacca
    22801 gtaccagagt ttcttcaacc aatgcattga ggctgacttg ttgccattga ctgtggcgtg
    22861 ggcgtgaaaa ttcgagcaat tgctgaataa ctttgttaat tgaatcgatt tctttgagta
    22921 ctacggacag atattcctga tgtatgaggt cgctggtttg ttggcgcaag atctgtacat
    22981 aaccacgaat agccgttagc ggattacgta cttcatgcgc cacgccagcc atcagctcac
    23041 ccagtgtagc gaggcgttct gcttgcgcca tgcggcgctg ggtttctttg cgggcagtta
    23101 aatcagagaa aatcaccaaa gcacctatca tttcaccatg cgtgttatga atacgactgg
    23161 tagtgacact gagttcaatg gtgcggtcac ggcctggaaa actgatctcc agcgctacat
    23221 gttcggtgcc atgttccagc gtatccagta ctggactgta gaactgagta ttgtcgaaca
    23281 acatggagta aggctgccct accagttcat ggcgttgata gccagtaata acttctgctg
    23341 ctgggttcat ggtggttaca tcaccctggc ggtcaatggc aatgacgcca tcggcagcgt
    23401 tttcaataat cagatcgtta agtgttcgcg tttcacgcag tgcctgggcg aggttgttaa
    23461 cactctgact gatttgcccc atttcaccgg gcaattgtgg taatcgagtg ggaatatttt
    23521 gtgccagagt cgagaggcca tcggtgatga tatcaatatt ggcgctcagg cgacgggaga
    23581 aaaggacaat caacagcagg cttatcagca aaccggcggt aagcacaatg ataatcctca
    23641 catccatttt ccaggcctgg cggcgaatat cttcggttaa ttcattggcc cagatatagc
    23701 cgaggatttc accattacgc tcaatgggga tcattgaatt caaaatatcg ccgcgcacct
    23761 gcctgcctga ataaaccaaa ggggtatttg tacgcatgac ttcgcgacca gggtgatctg
    23821 cggcaatggt aacgccgaca ttattctgat atagcgctga aggcgcgtag gtgattatcg
    23881 catccagcgt tttgttgtaa taaccagcgc cgatgccagg gaaggcgtga gtgatatttt
    23941 cggtaatggg ggcaagttct gcatttaatg cgcggatacg ctcctcacgt ggtaagtcga
    24001 tgtagagatc atagcgatcg cctagtgcct gattaagcag gttgaccacg gcagaaagtt
    24061 ttttctcttt ttcagataag actgctgaac gtccttccgt ttctacgata taaccaatag
    24121 taagcgttgg gacaatgacc atcaggattg ccatcaggat catttgattg cgtaagcggc
    24181 gtggataaat ccacttcata taatgcatgc tgaaatccac tagtcttgtc cggtatatga
    24241 cgattatcag aggttaaggt gatgatttct cggcggtgta tcatattcca gagaagagag
    24301 aacattgcgg taacacgctt ttaccgctac cttaaccaca ctccatcggt cacctgaggc
    24361 ggagcttcgc ccctttgaaa taccttgctt cttttcgtac aaccctgaaa gcctcgcgct
    24421 acatgttcag agcattggcg ttagtgctct ggctgttgat tgctttttca tccgtttttt
    24481 acatcgttaa tgcgttacat cagcgagaat cggaaattcg tcaggaattt aatctgagtt
    24541 ccgatcaggc tcagcgcttt attcaacgca cctctgatgt gatgaaagag ctgaagtaca
    24601 tcgccgaaaa tcgcttatcg gcagaaaacg gtgtgctttc cccgcgtgga cgagaaacgc
    24661 agacggatgt gcctgcgttt gaaccgctgt ttgctgactc tgattgttcc gcaatgagta
    24721 acacctggcg aggttctctg gagtcattgg cgtggtttat gcgctactgg cgcgataatt
    24781 tttctgcggc ttacgatcta aaccgggtat ttttaatcgg cagcgataac ctctgcatgg
    24841 ctaatttcgg tctgcgtgat atgccagtgg aacgcgatac cgcgttaaaa gctttgcatg
    24901 aacgcattaa taaatatcga aatgcaccac aagatgatag tggaagtaac ctctactgga
    24961 tcagcgaagg tccgcgccct ggcgtcgggt atttttacgc cttgacgcca gtttatctgg
    25021 cgaatcggtt gcaggcgctt ttgggtgtcg agcaaaccat ccggatggag aactttttct
    25081 tacctggtac gttgccgatg ggggttacca ttcttgatga aaatggtcat accctgattt
    25141 cgcttaccgg accagaaagc aaaattaagg gcgatcctcg ctggatgcag gaacgctcct
    25201 ggtttggcta tacggaaggg ttccgcgaac tggtgctgaa gaaaaatctg ccaccctcat
    25261 cgctaagcat cgtttattca gtgccagttg ataaggtgct ggaacgcatt cgcatgttga
    25321 tccttaacgc aattttgctg aatgtgcttg ccggagctgc attgtttact ctcgcgcgga
    25381 tgtacgagcg gcgtattttc attccggcgg aaagcgacgc cctgcgactg gaagaacatg
    25441 agcagtttaa tcgcaagatt gtcgcctccg cgccagtggg tatctgcatt ttgcgtaccg
    25501 ctgatggcgt caatatttta agtaacgaac tggcgcatac ctatctcaat atgcttacgc
    25561 atgaggaccg ccaacggctg acacaaatta tctgtgggca gcaggtcaat tttgttgatg
    25621 tcctgaccag caacaatacc aatctgcaaa tcagcttcgt ccattcgcgc tatcgtaatg
    25681 aaaacgtggc catttgtgtg ctggtggatg tttcttcgcg cgtgaagatg gaagagtcgt
    25741 tgcaggagat ggcacaagca gcggaacagg cgagccagtc aaaatcgatg ttcctggcca
    25801 ccgtcagtca tgagctgcga acgccgctgt atggcattat cggtaacctg gatctgttgc
    25861 aaaccaaaga gttaccgaaa ggcgtcgatc gtctggtgac ggcaatgaac aactcttcca
    25921 gcctgttgtt gaaaattatc agcgatattc tcgatttctc gaaaattgaa tcagaacagt
    25981 tgaagatcga accgcgtgag ttttcaccgc gtgaagtgat gaaccacatc accgccaact
    26041 atttaccgct ggtggtacgc aagcagttag gcttgtactg ctttattgaa ccgggtgtgc
    26101 cagtggcctt aaatggcgac ccgatgcgtt tacagcaggt catctccaac ctgttgagta
    26161 acgccataaa attcaccgat accggctgta tagttttgca tgttcgcgct gatggcgatt
    26221 atctctctat ccgtgttcgc gataccggcg tggggattcc ggcaaaagaa gtggtgcgtt
    26281 tgtttgatcc cttcttccag gtcggaacgg gcgtacagcg taacttccag gggactggtc
    26341 tgggtctggc gatttgtgaa aaactgatca gcatgatgga cggcgacatc tcggtagatt
    26401 cagaaccggg aatgggcagc cagtttaccg tgcgtattcc gttgtacggc gctcagtacc
    26461 cgcagaaaaa aggtgtggaa gggttgagtg gtaaacgctg ctggctggcg gtccgcaatg
    26521 cgtcgctctg tcagttcctg gaaaccagtt tgcagcgcag cggcatcgtc gttacaacat
    26581 acgaagggca ggaaccgact cccgaagatg tgttaattac tgacgaggta gtgaacaaaa
    26641 aatggcaggg cagagcggta gtgaccttct gtcgtcgcca tattggtatt ccgctggaga
    26701 aagcgccagg ggagtgggta cacagtgtgg ctgctccgca tgagctaccg gcattgttgg
    26761 cgcgtattta tttgatcgag atggagagcg acgatcctgc taacgctctg ccgtcgacgg
    26821 acaaagcggt cagcgataat gacgatatga tgattctggt cgtggatgat catccgatta
    26881 accggcgtct gctggcagat cagttgggat cgttaggcta tcaatgtaaa accgcgaatg
    26941 atggcgtcga tgcgcttaat gtacttaaca agaatcatat tgatatcgtg cttagcgacg
    27001 tcaatatgcc aaatatggat ggttaccgct tgacgcaacg cattcgtcag ttgggactga
    27061 cgttgccggt aatcggagta actgctaatg cgttggctga agagaagcag cggtgtctgg
    27121 agtccggtat ggacagttgc ctgtcgaagc cggtaacgct ggatgtgata aaacagacgc
    27181 tgacggtata tgccgagagg gtcaggaaat cgcgggaatc gtaggacgga taaggcgttt
    27241 acgccgcatc cggcaatccg tgcaaatgcc agataagaca ctaacgcgtc ttatctggcc
    27301 tacaggtgat tagtctttat ctgccggact taacgtcaca gaagagagat aattcagcag
    27361 agcaatatcg ttctcgacac ccagcttcat catcgcagat ttcttctggc tactgatggt
    27421 tttaatactg cggttcagct ttttagcgat ctcggtcacc aggaagcctt ccgcaaacag
    27481 gcgcagaact tcactctctt ttggcgagag acgcttgtca ccataaccac cagcactgat
    27541 tttttccaac aggcgagaaa cgctttccgg ggtaaatttc ttccctttct gcagcgcggc
    27601 gagagctttc ggcagatcgg tcggtgcacc ttgtttcagc acgatccctt cgatatccag
    27661 atccaatacc gcactaagaa tcgccgggtt gttgttcatg gtcagaacaa tgatcgacag
    27721 gcttgggaaa tggcgcttga tgtacttgat taaggtaatg ccatcgccgt acttatcgcc
    27781 aggcatggag agatcggtaa tcaacacatg tgcatccagt ttcggcaagt tgttgatcag
    27841 tgctgtagag tcttcaaatt cgccgacaac attcacccac tcaatttgct caagtgattt
    27901 gcgaataccg aacaagacta tcggatggtc atcggcaata attacgttca tattgttcat
    27961 gtaataggct accttgctac agcaagctct tgacataact gtcaatgtcg ctgatgtatt
    28021 tttctattcc tggaacatcc ttctcacgaa tcagatgttc cagcgtttca cataactgct
    28081 tgccgggtac cagatttagc atggcaaata cgcctttaag acgatgagcc gtttgggcta
    28141 acgcagcaaa gtcactggtt gctgcttcag tatatagcct cttaacatca tccggtactg
    28201 tgtctacaaa gagcgcataa tagccgctgg catggagttg cgcattttca tctccgccca
    28261 gaggcgattc tgtcacctct tcctgcgcca gttgcacttc aattaattgt aagaccgctt
    28321 cctgcatagc gttgctcata ttgaaattga cgcacaattg accaggccca atttcccgta
    28381 cgccagactc atcatcgctt aaaagcaagc cagaggcagt aagattagac ggattatccg
    28441 ttaaaaagat atcataatct tgactaatta atctttcatc gggtgtgata caggttgcac
    28501 cccaattttc taactggcga gtgacaatat tccgaatttc tgccgaagta acatccacca
    28561 ttacgcagac atcatccagt aatcgctctt cttcctcttc aacttccggg tcagctgcga
    28621 gcattttgat atgcacagag tagcgtgtac caagcccatc ccgcgttttg atgtttaaat
    28681 gaccgcccag tttacgcgcc agttgatcgc tcagccagaa tgccagcggg tccgccttgc
    28741 catagcgatc gttttgggtc tggttgataa acgggaagtg caaattatcc atttcatgaa
    28801 tgcttacgcc ttctccagtg tccagaatgc ggaacgtcag gcggtcttcg gaggactcat
    28861 cctgatcaac ctcaagggtg atttttccca attgcgttga ggtcacggca tattgcatca
    28921 gtagtagcaa aatacgtcgt aaggcatcgc gatcgccgcg gcgcatatcg tgtgctttca
    28981 gatgattgtt aatcagcagt tgcagacctt tacgcttgat ggcaggcaac actgaaggca
    29041 caacttcatc aattaaatcc tgcacggaga acagctccgt ctcacttttc cagctatcgt
    29101 ccgcaagcat gttcgctaac tgtatttcat cgaccagccg caccagcaca tctgcctgat
    29161 tcgccagttg tttgccttcc ggggcgttga gtttagccgc gctctccgcc agggactgtg
    29221 cgggttcttt cagcgcatcg ccaatgtttt tcataaaggt catccgcccc tgctggtttt
    29281 tctcatacag acgctgcgcc tgcttgagtt tcttgtttac cagcacttcg cgatcctgat
    29341 cgcgaataat gaaaatttgt gtgcgcggcg caacctggct gcggaacatg cggatctcat
    29401 acagctcgtt attgatcgtc gcctgaataa tcccctgatg ctgttccgcc atggtggtga
    29461 tgttttgcag attcaaatgc ggcagcaaat gatcggcaat tttgttactt atgactgtgc
    29521 ggttcgattc ctgatcgtga accagcaggc cgagcggcag cagtgagact atctcttcat
    29581 taattgcccg taaaatgcgc aattcgttat tgaccgccgt gctggggacg ctttctgtac
    29641 tgcggctgga gaaatggcgg aatgtggtat agccaaataa cgccagcgcc agcaaaccga
    29701 tgttcagcag cagtggcagc agaatgtttt gcaacgtatc cagcagtaag gtgccgtaag
    29761 gaacctgcca gaccaggcgc atatcggtag agttgagtgc cgaggagatt tcaatcttcg
    29821 tactgttaaa gtggatactg acgctatccg tcccttcttt ctcattttca ttgtttcccg
    29881 ttgccgtcgc gtctggctca aggcggaaac tgtccagcgg catacccggt gggatcaaat
    29941 cattaatcgg cagatcaaaa gccacgaccg ttgccagatg tcccggctgg ttgaacgtgg
    30001 tacgcaaggt aaagtaatga ccgttctgcc aggccaggcg gcgcatgtta gaaaaacttt
    30061 cgcgttcatc gagggcgttg gcctgttgta acatctctgc acgacgtgaa tcaacaatgt
    30121 cgctgatggt cgattcttta aatccggagg tgagatcttt gaggggcagg gttgagatca
    30181 gcaccagact gttatcctga ccattcaggt aatacatcga ccacggtaca ttttctgcgc
    30241 cccacaatgt gtccagatag gtggacatcc gttgagtcat ctcaagcgtt gagttgtcat
    30301 gagagccaaa gattaacgct tccgttttgc ggcgtggttt ctccagatag tagacatcct
    30361 gtttcaggcg cgtctcttgt aaaccttcgc cggaggaggg ggaggtcgtc gcggcaatgt
    30421 tgtcgtagat ctgccaggtc acgtaacgcc aggtatcgac gcgcttttgg attgcgtggg
    30481 taatgtcgac aatctggtaa cttttatctt tcagccaggc gttaacggcg ctttgtacca
    30541 ttacacccat cgtcaccagt aacacaatga tcaacagtaa aaagaagcgg gtaatgctcc
    30601 ccggtaggag tgaaaagcgg gtcgtggccg ttgtctcttt ctgacgcatt cgtgtttatg
    30661 acctgttaaa acttcgcgaa gtgatagggc agtataaagg gtacagtgtg aaattccaga
    30721 ctcttacctg attgatgatt ctctattctc cagtgatatt tatcatcctg cattggggga
    30781 aatagatgta caagcgccat tttgctgtac aaataaccta caaaaagcct aacgaaatta
    30841 attgcaaata aacgaagatt gctggaaatt atgctgatgt taattatttg tgaaatagtt
    30901 aacgagcgtt atagtttttc tgtggtagca cagaataatg aaaagtgtgt aaagaagggt
    30961 aaaaaaaacc ggatgcgagg catccggttg aaataggggt aaagagacat tcagaaatga
    31021 atgacggtaa taaataaagt taatgatgat agcggaagat attctagttg cgagtgaagg
    31081 ttttgttttg acattcagtg ctgtcaaata attaagaata agttattgat tttaatcttg
    31141 aattaatatt gcttgatgtt aggtgcttat ttggccattc cgcaataatc ttaaaaagtt
    31201 cccttgcatt tacattctga aacatctata gcgataaatg aaacatctta aaagttttag
    31261 tatcatattc gtgttggatt attctgcatt tttggggaga atggacttgc cgactgatta
    31321 atgagggtta atcagtatgc agtggcataa aaaagcaaat aaaggcatat aacagagggt
    31381 taataacatg aaagttaaag tactgtccct cctggtccca gctctgctgg tagcaggcgc
    31441 agcaaacgct gctgaagttt acaacaaaga cggcaacaaa ttagatctgt acggtaaagt
    31501 agacggcctg cactatttct ctgacgacaa gtctgtagat ggcgaccaga cctacatgcg
    31561 tcttggcttc aaaggtgaaa ctcaggttac tgaccaactg accggttacg gccagtggga
    31621 atatcagatc cagggcaaca gcgctgaaaa cgaaaacaac tcctggaccc gtgtggcatt
    31681 cgcaggtctg aaattccagg atgtgggttc tttcgactac ggtcgtaact acggcgttgt
    31741 ttatgacgta acttcctgga ccgacgtcct gccagaattc ggtggcgaca cttacggttc
    31801 tgacaacttc atgcagcagc gtggtaacgg cttcgcgacc taccgtaaca ccgacttctt
    31861 cggtctggtt gacggcctga actttgctgt tcagtaccag ggcaaaaacg gtagcgtaag
    31921 cggcgaaggc atgaccaaca acggtcgtga tgctctgcgt cagaacggcg acggcgttgg
    31981 cggttctatc acttatgatt acgaaggctt cggtatcggt ggtgcgatct ccagctccaa
    32041 acgtactgat gctcagaaca ccgctgctta catcggtaac ggcgaccgtg ctgaaaccta
    32101 cactggtggt ctgaaatacg acgctaacaa catctacctg gctgctcagt acacccagac
    32161 ctacaacgca actcgcgtag gttccctggg ttgggcgaac aaagcacaga acttcgaagc
    32221 tgttgctcag taccagttcg acttcggtct gcgtccgtcc ctggcttacc tgcagtctaa
    32281 aggtaaaaac ctgggcacca tcggtactcg taactacgac gacgaagata tcctgaaata
    32341 tgttgatgtt ggtgctactt actacttcaa caaaaacatg tccacctatg ttgactacaa
    32401 aatcaacctg ctggacgaca accagttcac tcgtgacgct ggcatcaaca ctgataacat
    32461 cgtagctctg ggtctggttt accagttcta atctcgatgg atatcgaaca aagggcctgc
    32521 gggccctttt ttcattgttt tcagcgtaca aacacagttt tttggtgtac tcttgcgacc
    32581 gttcgcatga ggataatcac gtatggaaat aagctttacc cgcgtggcac tgctggctgc
    32641 cgcgctcttc tttgttggtt gcgatcaaaa accacaaccc gccaaaaccc acgctactga
    32701 agttaccgtt cttgaaggca aaaccatggg taccttctgg cgtgccagca tcccgggcat
    32761 tgacgccaaa cgcagtgccg aacttaaaga aaagattcag acccaactgg acgctgacga
    32821 tcagctgctt tcgacctata aaaaagattc cgcgctgatg cgctttaacg actcgcaaag
    32881 tttgtctccg tggccggtaa gtgaagcgat ggccgatatc gtcaccacct cgctgcgcat
    32941 tggcgcgaag accgatggcg cgatggatat aaccgtcggg ccgctggtga atctgtgggg
    33001 ttttggcccg gaacaacagc cggttcaaat tccgagccag gaacagatcg atgcgatgaa
    33061 agccaaaacc ggcttacagc acctgacggt cattaatcag tcgcatcagc aatatctgca
    33121 aaaagacctg ccggatttat atgtcgatct ctccaccgtc ggcgaaggtt atgcggcgga
    33181 tcacctggca cgcttgatgg agcaggaagg gatttcccgc tatctggtgt cggtgggcgg
    33241 cgcgctgaac agccgtggta tgaacggtgc tggcaagccg tggcgggtgg cgattcaaaa
    33301 accaaccgat aaagaaaacg cggttcaggc cgtggtggat atcaacggtc atgggattag
    33361 tacctctggc agctaccgta actattacga actggacggc aaacgtcttt ctcatgttat
    33421 cgatccgcaa accgggcgtc ctatcgaaca caatctcgta tccgtgacgg tgattgcccc
    33481 gacggcgctg gaagccgatg cctgggatac tggcttaatg gtactcggac cggagaaagc
    33541 caaagaggtt gttcgccggg aaggactggc ggtctacatg atcaccaaag agggcgataa
    33601 ttttaaaacc tggatgtcgc cgcagtttaa aagcttcctt atcagcgaaa aaaattaaag
    33661 cgcaagattg ttggtttttg cgcgatggtg accgggcaga ctaacggcta tccttaacca
    33721 gggagctgat tatgaaaaac gccacatgtt taactgacga tcaacgctgg caatctgtct
    33781 tagcccgcga cccgaacgcc gacggcgaat tcgttttcgc cgtgcgcacc acgggtatct
    33841 tttgccgtcc gtcttgccga gccagacatg ccttgcggga aaatgtctcc ttctacgcaa
    33901 atgccagcga ggcgcttgcc gctggctttc gtccctgcaa acgttgtcag ccagacaaag
    33961 ccaatccccg gcaacatcgc cttgataaaa ttacccacgc ctgccgactt ctggaacagg
    34021 aaacgcctgt aacgctggaa gccttagccg accaggtggc gatgagtcca tttcatttgc
    34081 atcggttgtt taaagcgact accggaatga cgcctaaagc ctggcaacag gcctggcgcg
    34141 ctcgccgttt gcgcgaattg ctggcgaaag gggagagcgt gactacatct attcttaacg
    34201 ccggattccc cgacagcagc agttactacc gcaaagccga cgaaacgctg ggcatgacgg
    34261 ctaaacaatt ccgtcacggt ggcgaaaatc tggcggtgcg ttacgcgctg gctgattgtg
    34321 agctgggccg ttgcctggtg gcagaaagtg agcgggggat ttgcgcgata ttactgggcg
    34381 atgatgacgc gacactaatc agcgagttac agcagatgtt tcccgctgcc gacagcgcgc
    34441 ctgccgatct tacgtttcag caacatgtgc gtgaagtgat cgccagcctc aatcaacgcg
    34501 atacgccgct gacgttacca ctggacattc gcggcactgc ttttcagcaa caagtctggc
    34561 aggcactgcg cacgatacct tgcggtgaaa ccgtcagtta tcagcaactg gctaacgcca
    34621 ttggcaaacc gaaagcggta cgggccgttg ccagcgcctg tgccgccaac aagctagcta
    34681 tcgttatacc ttgtcatcgg gtggtccgtg gtgatggcac actttccggt taccgctggg
    34741 gcgtgtcgcg caaagcgcaa ctgctgcgcc gcgaagctga aaatgaggaa aggtaatgtt
    34801 ggatctgttt gccgatgctg aaccgtggca agaaccactg gcggctggag cggtgatttt
    34861 gcaccgtttt gcttttaacg ctgcggagca actgatccgc gatattaatg acgttgccag
    34921 ccagtcgccg tttcgccaga tggtcactcc aggcggatat accatgtcgg tggcgatgac
    34981 caactgtggg catctgggct ggacgaccca tcggcaaggt tatctctatt cgcccattga
    35041 tccgcaaaca aataaaccgt ggcccgccat gccgcagagt tttcatcatt tatgtcaacg
    35101 tgcggctacg gcagcgggct atccagattt ccagccagat gcctgtttga tcaaccgcta
    35161 cgttcctggc gcgaaactgt cgctgcatca ggataaagac gaaccggatc tgcgcgcgcc
    35221 aattgtttct gtttctctgg gcttacccgc gatttttcaa tttggcggcc tgaaacgaaa
    35281 tgatccgctc aaacgtttgt tgttggaaca tggcgatgtg gtggtatggg gggggtgagt
    35341 cgcggctgtt ttatcatggc attcaaccgt tgaaagtggg ggttcatcca ctcaccaccg
    35401 actgccgcta caacctgaca ttccgtcagg caggtaaaaa agaataaaaa taagaattat
    35461 tattgctgtg cgcacgaaga tgtttaaact gcgggctgta attcattgtc cgggttttct
    35521 gcatggaact tcttgtactt gtctggcggc agtatcgctg gccatttatc agtgtgatgg
    35581 cgctaagcct cgccagtgcg gcattaggca ttggcttaat tgcttttatc aatcagcgcc
    35641 ttatcgaaac ggcggatacc agtctgctgg tattgccgga gtttctgggg ttattgctgc
    35701 tgttgatggc cgtcactctc ggatcgcaac tggcgctcac cactttgggg catcacttcg
    35761 tttaccgact gcgtagtgaa tttatcaagc ggattctcga tactcacgtc gagcgcattg
    35821 aacaactcgg tagcgcctcg ttgctggcgg gattaaccag cgatgtgcgc aatatcacca
    35881 ttgcttttgt gcgtctgccg gaactggtgc aggggatcat tctcactatc ggttcagcgg
    35941 cgtatttgtg gatgctgtcg ggcaaaatgt tgctggtaac ggctatctgg atggcagtca
    36001 ccatctgggg cggttttgtg ctggtggcgc gggtgtacaa acatatggcg accctgcgtg
    36061 aaaccgaaga taagctgtac acggattttc aaactgtact cgaagggcgc aaagagctga
    36121 ctctgaaccg tgaacgcgcc gagtatgtgt ttaacaacct ctacattcct gatgcgcaag
    36181 agtatcgcca tcatattatc cgcgcagaca ccttccatct tagtgccgtg aactggtcaa
    36241 acatcatgat gctgggcgca atcggcctgg tgttctggat ggcgaacagt ctcggttggg
    36301 ctgataccaa cgttgccgcg acctattcgt tgacgctgtt attcctgcgt acgccgctgc
    36361 tttcggcggt tggcgcatta ccgacgctgc tgacggcgca ggtggcgttt aacaagctga
    36421 acaaattcgc gctcgcgcct ttcaaagcag agtttccgcg cccgcaggcg tttcccaact
    36481 ggcaaacgct ggagctgcgt aacgtgacgt ttagctatca ggataacgcg ttttccgttg
    36541 ggccgattaa cctcaccatt aaacgtggcg agctgctgtt tctgattggt ggcaacggta
    36601 gtggaaaatc gacgctggcg atgttgctga cgggcttgta ccagccacaa aatggcgaaa
    36661 tcttgctgga tggcaaacct gtcagcggcg aacaaccgga agattatcga aaactgtttt
    36721 cggcagtgtt taccgatgtc tggctgtttg atcaactgct ggggccggag gggcaacccg
    36781 ctaacccgca actggttgag aagtggctgg tgcagctgaa aattgctcat aagcttgagt
    36841 taagcaacgg gcgtattgtt aacctgaagt tatcaaaagg gcagaaaaaa cgcgtggcgc
    36901 tgttgctggc gctggcagaa gaacgcgata ttatcctgct ggatgaatgg gcggcggatc
    36961 aggatccaca cttccgtcgt gagttttatc aggtgctgct gccgctgatg caggagatgg
    37021 gtaaaactat tttcgccatc agtcatgatg atcattactt tatccacgcc gaccgcctgc
    37081 tggaaatgcg caacgggcaa cttagcgagc tgacaggcga agagcgcgat gctgcttcgc
    37141 gtgatgccgt tgcccggacg gcataatatt acgacgctga cctggcggac tgctgccgcc
    37201 aggtcaatat tctaaaaata ccttcgtttc taa
//
LOCUS       DADSYM010000024        37022 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2415
            SAMN08039579-rid11723173.denovo.024, whole genome shotgun sequence.
ACCESSION   DADSYM010000024 DADSYM010000000
VERSION     DADSYM010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039579
            Sequence Read Archive: SRR6362533
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 37022)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37022)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980162.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 03:39:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,484
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 4,347
            CDSs (with protein)               :: 4,347
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 43 of 85
            Pseudo Genes (incomplete)         :: 37 of 85
            Pseudo Genes (internal stop)      :: 19 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37022
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039579-rid11723173.denovo.024"
                     /strain="LGE2415"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8302 N 95.9628 W"
                     /collection_date="2015-08-24"
     gene            <1..435
                     /gene="tnpA"
                     /locus_tag="J1Y83_001034"
     CDS             <1..435
                     /gene="tnpA"
                     /locus_tag="J1Y83_001034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HBA7629205.1"
                     /translation="HTRWNCKYHIVFAPKYRRQVFYREKRRAIGSILRKLCEWKSVRI
                     LEAECCADHIHMLVEIPPKMSVSGFMGYLKGKSSLMLYEQFGDLKFKYRNREFWCRGY
                     YVDTVGKNTAKIQDYIKHQLEEDKMGEQLSIPYPGSPFTGRK"
     gene            complement(524..1566)
                     /locus_tag="J1Y83_001035"
                     /pseudo
     CDS             complement(524..1566)
                     /locus_tag="J1Y83_001035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000547268.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
     gene            complement(1692..4367)
                     /gene="adhE"
                     /locus_tag="J1Y83_001036"
                     /gene_synonym="adhC"
     CDS             complement(1692..4367)
                     /gene="adhE"
                     /locus_tag="J1Y83_001036"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HBA7629206.1"
                     /translation="MAVTNVAELNALVERVKKAQREYASFTQEQVDKIFRAAALAAAD
                     ARIPLAKMAVAESGMGIVEDKVIKNHFASEYIYNAYKDEKTCGVLSEDDTFGTITIAE
                     PIGIICGIVPTTNPTSTAIFKSLISLKTRNAIIFSPHPRAKDATNKAADIVLQAAIAA
                     GAPKDLIGWIDQPSVELSNALMHHPDINLILATGGPGMVKAAYSSGKPAIGVGAGNTP
                     VVIDETADIKRAVASVLMSKTFDNGVICASEQSVVVVDSVYDAVRERFATHGGYLLQG
                     KELKAVQDVILKNGALNAAIVGQPAYKIAELAGFSVPENTKILIGEVTVVDESEPFAH
                     EKLSPTLAMYRAKDFEDAVEKAEKLVAMGGIGHTSCLYTDQDNQPARVSYFGQKMKTA
                     RILINTPASQGGIGDLYNFKLAPSLTLGCGSWGGNSISENVGPKHLINKKTVAKRAEN
                     MLWHKLPKSIYFRRGSLPIALDEVITDGHKRALIVTDRFLFNNGYADQITSVLKAAGV
                     ETEVFFEVEADPTLSIVRKGAELANSFKPDVIIALGGGSPMDAAKIMWVMYEHPETHF
                     EELALRFMDIRKRIYKFPKMGVKAKMIAVTTTSGTGSEVTPFAVVTDDTTGQKYPLAD
                     YALTPDMAIVDANLVMDMPKSLCAFGGLDAVTHAMEAYVSVLASEFSDGQALQALKLL
                     KEYLPASYHEGSKNPVARERVHSAATIAGIAFANAFLGVCHSMAHKLGSQFHIPHGLA
                     NALLICNVIRYNANDNPTKQTAFSQYDRPQARRRYAEIADHLGLSAPGDRTAAKIEKL
                     LAWLETLKAELGIPKSIREAGVQEADFLANVDKLSEDAFDDQCTGANPRYPLISELKQ
                     ILLDTYYGRDYVEGETAAKKEAAPAKAEKKAKKSA"
     gene            4844..5491
                     /gene="ychE"
                     /locus_tag="J1Y83_001037"
     CDS             4844..5491
                     /gene="ychE"
                     /locus_tag="J1Y83_001037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter YchE"
                     /protein_id="HBA7629207.1"
                     /translation="MIQTLFDFPVYFKFFIGLFALVNPVGIIPVFISMTSYQTAAARN
                     KTNLTANLSVAIILWISLFLGDTILQLFGISIDSFRIAGGILVVTIAMSMISGKLGED
                     KQNKQEKSETAVRESIGVVPLALPLMAGPGAISSTIVWGTRYHSISYLFGFFVAIALF
                     ALCCWGLFRMAPWLVRVLRQTGINVITRIMGLLLMALGIEFIVTGIKGIFPGLLN"
     gene            complement(5649..5810)
                     /locus_tag="J1Y83_001038"
     CDS             complement(5649..5810)
                     /locus_tag="J1Y83_001038"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA7629208.1"
                     /translation="MSLFSDYSSSREMHNNLTIDYYLALSSTKGSGITNIISIILQQA
                     QDYDVAKIT"
     gene            6229..7860
                     /gene="oppA"
                     /locus_tag="J1Y83_001039"
     CDS             6229..7860
                     /gene="oppA"
                     /locus_tag="J1Y83_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707150.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter substrate-binding
                     protein OppA"
                     /protein_id="HBA7629209.1"
                     /translation="MTNITKRSLVAAGVLAALMAGNVALAADVPAGVTLAEKQTLVRN
                     NGSEVQSLDPHKIEGVPESNISRDLFEGLLVSDLDGHPAPGVAESWDNKDAKVWTFHL
                     RKDAKWSDGTPVTAQDFVYSWQRSVDPNTASPYASYLQYGHIAGIDEILEGKKPITDL
                     GVKAIDDHTLEVTLSEPVPYFYKLLVHPSTSPVPKAAIEKFGEKWTQPGNIVTNGAYT
                     LKDWVVNERIVLERSPTYWNNAKTVINQVTYLPIASEVTDVNRYRSGEIDMTYNNMPI
                     ELFQKLKKEIPDEVHVDPYLCTYYYEINNQKPPFNDVRVRTALKLGMDRDIIVNKVKA
                     QGDMPAYGYTPPYTDGAKLTQPEWFGWSQEKRNEEAKKLLAEAGYTADKPLTINLLYN
                     TSDLHKKLAIAASSLWKKNIGVNVKLVNQEWKTFLDTRHQGTFDVARAGWCADYNEPT
                     SFLNTMLSNSSMNTAHYKSPAFDSIMAETLKVTDEAQRTALYTKAEQQLDKDSAIVPV
                     YYYVNARLVKPWVGGYTGKDPLDNTYTRNMYIVKH"
     gene            7946..8866
                     /gene="oppB"
                     /locus_tag="J1Y83_001040"
     CDS             7946..8866
                     /gene="oppB"
                     /locus_tag="J1Y83_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease OppB"
                     /protein_id="HBA7629210.1"
                     /translation="MLKFILRRCLEAIPTLFILITISFFMMRLAPGSPFTGERTLPPE
                     VMANIEAKYHLNDPIMTQYFSYLKQLAHGDFGPSFKYKDYSVNDLVASSFPVSAKLGA
                     AAFFLAVILGVSAGVIAALKQNTKWDYTVMGLAMTGVVIPSFVVAPLLVMIFAIILHW
                     LPGGGWNGGALKFMILPMVALSLAYIASIARITRGSMIEVLHSNFIRTARAKGLPMRR
                     IILRHALKPALLPVLSYMGPAFVGIITGSMVIETIYGLPGIGQLFVNGALNRDYSLVL
                     SLTILVGALTILFNAIVDVLYAVIDPKIRY"
     gene            8881..9789
                     /gene="oppC"
                     /locus_tag="J1Y83_001041"
     CDS             8881..9789
                     /gene="oppC"
                     /locus_tag="J1Y83_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000979653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease OppC"
                     /protein_id="HBA7629211.1"
                     /translation="MMLSKKNSETLENFSEKLEVEGRSLWQDARRRFMHNRAAVASLI
                     VLVLIALFVILAPMLSQFAYDDTDWAMMSSAPDMESGHYFGTDSSGRDLLVRVAIGGR
                     ISLMVGVAAALVAVVVGTLYGSLSGYLGGKVDSVMMRLLEILNSFPFMFFVILLVTFF
                     GQNILLIFVAIGMVSWLDMARIVRGQTLSLKRKEFIEAAQVGGVSTPGIVIRHIVPNV
                     LGVVVVYASLLVPSMILFESFLSFLGLGTQEPLSSWGALLSDGANSMEVSPWLLLFPA
                     GFLVVTLFCFNFIGDGLRDALDPKDR"
     gene            9801..10814
                     /gene="oppD"
                     /locus_tag="J1Y83_001042"
     CDS             9801..10814
                     /gene="oppD"
                     /locus_tag="J1Y83_001042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide/oligopeptide ABC transporter
                     ATP binding protein OppD"
                     /protein_id="HBA7629212.1"
                     /translation="MSVIETATVPLAQQQADALLNVKDLRVTFSTPDGDVTAVNDLNF
                     SLRAGETLGIVGESGSGKSQTAFALMGLLAANGRIGGSATFNGREILNLPERELNKLR
                     AEQISMIFQDPMTSLNPYMRVGEQLMEVLMLHKNMSKAEAFEESVRMLDAVKMPEARK
                     RMKMYPHEFSGGMRQRVMIAMALLCRPKLLIADEPTTALDVTVQAQIMTLLNELKREF
                     NTAIIMITHDLGVVAGICDKVLVMYAGRTMEYGNARDVFYQPVHPYSIGLLNAVPRLD
                     AEGETMLTIPGNPPNLLRLPKGCPFQPRCPHAMEICSSAPPLEEFTPGRLRACFKPVE
                     ELL"
     gene            10811..11815
                     /gene="oppF"
                     /locus_tag="J1Y83_001043"
     CDS             10811..11815
                     /gene="oppF"
                     /locus_tag="J1Y83_001043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide/oligopeptide ABC transporter
                     ATP binding protein OppF"
                     /protein_id="HBA7629213.1"
                     /translation="MNAVTEGRKVLLEIADLKVHFEIKDGKQWFWQPPKTLKAVDGVT
                     LRLYEGETLGVVGESGCGKSTFARAIIGLVKATDGHVAWLGKELLGMKPDEWRAVRSD
                     IQMIFQDPLASLNPRMTIGEIIAEPLRTYHPKMSRQEVRERVKAMMLKVGLLPNLINR
                     YPHEFSGGQCQRIGIARALILEPKLIICDEPVSALDVSIQAQVVNLLQQLQREMGLSL
                     IFIAHDLAVVKHISDRVLVMYLGHAVELGTYDEVYHNPLHPYTRALMSAVPIPDPDLE
                     KNKTIQLLEGELPSPINPPSGCVFRTRCPIAGPECAKTRPVLEGSFRHAVSCLKVDPL
                     "
     gene            complement(11868..12197)
                     /locus_tag="J1Y83_001044"
     CDS             complement(11868..12197)
                     /locus_tag="J1Y83_001044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciU family protein"
                     /protein_id="HBA7629214.1"
                     /translation="MDMDLNNRLTEDETLEQAYDIFLELAADNLDPADVLLFNLQFEE
                     RGGAELFDPAEDWQEHVDFDLNPDFFAEVVIGLADSEDGEINDVFARILLCREKDHKL
                     CHIIWRE"
     gene            complement(12232..13692)
                     /gene="cls"
                     /locus_tag="J1Y83_001045"
     CDS             complement(12232..13692)
                     /gene="cls"
                     /locus_tag="J1Y83_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HBA7629215.1"
                     /translation="MTTVYTLVSWLAILGYWLLIAGVTLRILMKRRAVPSAMAWLLII
                     YILPLVGIIAYLAVGELHLGKRRAERARAMWPSTAKWLNDLKACKHIFAEENSSVAAP
                     LFKLCERRQGIAGVKGNQLQLMTESDDVMQALIRDIQLARHNIEMVFYIWQPGGMADQ
                     VAESLMAAARRGIHCRLMLDSAGSVAFFRSPWPELMRNAGIEVVEALKVNLMRVFLRR
                     MDLRQHRKMIMIDNYIAYTGSMNMVDPRYFKQDAGVGQWIDLMARMEGPIATAMGIIY
                     SCDWEIETGKRILPPPPDVNIMPFEQASGHTIHTIASGPGFPEDLIHQALLTAAYSAR
                     EYLIMTTPYFVPSDDLLHAICTAAQRGVDVSIILPRKNDSMLVGWASRAFFTELLAAG
                     VKIYQFEGGLLHTKSVLVDGELSLVGTVNLDMRSLWLNFEITLAIDDKGFGADLAAVQ
                     DDYISRSRLLDARLWLKRPLWQRVAERLFYFFSPLL"
     gene            13835..14008
                     /locus_tag="J1Y83_001046"
     CDS             13835..14008
                     /locus_tag="J1Y83_001046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciY family protein"
                     /protein_id="HBA7629216.1"
                     /translation="MKRSRTEVGRWRMQRQASRRKSRWLEGQSRRNMRIHSIRKCILN
                     KQRNSLLFAIYNI"
     gene            complement(14063..15316)
                     /gene="kch"
                     /locus_tag="J1Y83_001047"
     CDS             complement(14063..15316)
                     /gene="kch"
                     /locus_tag="J1Y83_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated potassium channel protein"
                     /protein_id="HBA7629217.1"
                     /translation="MSHWTTFKQTATKLWVTLRHDILALAVFLNGLLIFKTIYGMSVN
                     LLDIFHIKAFSELDLSLLANAPLFMLGVFLVLNSIGLLFRAKLAWAISIILLLIALIY
                     TLHFYPWLKFSIGFCIFTLVFLLILRKDFSHSSAAAGTIFAFISFTTLLFYSTYGALY
                     LSEGFNPRIESLMTAFYFSIETMSTVGYGDIVPVSESARLFTISVIISGITVFATSMT
                     SIFGPLIRGGFNKLVKGNNHTMHRKDHFIVCGHSILAINTILQLNQRGQNVTVISNLP
                     EDDIKQLEQRLGDNADVIPGDSNDSSVLKKAGIDRCRAILALSDNDADNAFVVLSAKD
                     MSSDVKTVLAVSDSKNLNKIKMVHPDIILSPQLFGSEILARVLNGEEINNDMLVSMLL
                     NSGHGIFSDNDEQETKADSKESAQK"
     gene            complement(15616..15912)
                     /locus_tag="J1Y83_001048"
     CDS             complement(15616..15912)
                     /locus_tag="J1Y83_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009448315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HBA7629218.1"
                     /translation="MLYVIYAQDKADSLEKRLSVRPAHLARLQLLHDEGRLLTAGPMP
                     AVDSNDPGAAGFTGSTVIAEFESLEAAQAWADADPYVAAGVYEHVSVKPFKKVF"
     gene            16136..16852
                     /gene="tonB"
                     /locus_tag="J1Y83_001049"
     CDS             16136..16852
                     /gene="tonB"
                     /locus_tag="J1Y83_001049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB system transport protein TonB"
                     /protein_id="HBA7629219.1"
                     /translation="MTLDLPRRFPWPTLLSVCIHGAVVAGLLYTSVHQVIELPAPAQP
                     ISVTMVAPADLEPPQAVQPPPEPVVEPEPEPEPIPEPPKEAPVVIEKPKPKPKPKPKP
                     VKKVEQPKRDVKPVESRPASPFENTALARPTSSTATAATSKPVTSVASGPRALSRNQP
                     QYPARAQALRIEGQVKVKFDVTPDGRVDNVQILSAKPANMFEREVKNAMRRWRYEPGK
                     PGSGIVVNILFKINGTTEIQ"
     gene            complement(16892..17290)
                     /gene="yciA"
                     /locus_tag="J1Y83_001050"
     CDS             complement(16892..17290)
                     /gene="yciA"
                     /locus_tag="J1Y83_001050"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioester hydrolase YciA"
                     /protein_id="HBA7629220.1"
                     /translation="MSTTHNVPQGDLVLRTLAMPADTNANGDIFGGWLMSQMDIGGAI
                     LAKEIAHGRVVTVRVEGMTFLRPVAVGDVVCCYARCVQKGTTSVSINIEVWVKKVASE
                     PIGQRYKATEALFKYVAVDPEGKPRALPVE"
     gene            complement(17396..17935)
                     /locus_tag="J1Y83_001051"
     CDS             complement(17396..17935)
                     /locus_tag="J1Y83_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation protein A"
                     /protein_id="HBA7629221.1"
                     /translation="MKQFLDFLPLVVFFAFYKIYDIYAATAALIVATAIVLIYSWVRF
                     RKVEKMALITFVLVVVFGGLTLFFHNDEFIKWKVTVIYALFAGALLVSQWVMKKPLIQ
                     RMLGKELTLPQSVWSKLNLAWAVFFILCGLANIYIAFWLPQNIWVNFKVFGLTALTLI
                     FTLLSGIYIYRHMPQEDKS"
     gene            complement(17965..18708)
                     /locus_tag="J1Y83_001052"
     CDS             complement(17965..18708)
                     /locus_tag="J1Y83_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0259 family protein"
                     /protein_id="HBA7629222.1"
                     /translation="MSITAQSVYRDTGNFFRNQFMTILLVSLLCAFITVVLGHVFSPS
                     DAQLAQLNDGVPVSGSSGLFDLVQNMSPEQQQILLQASAASTFSGLIGNAILAGGVIL
                     IIQLVSAGQRVSALRAIGASAPILPKLFILIFLTTLLVQIGIMLVVVPGIIMAILLAL
                     APVMLVQDKMGVFASMRSSMRLTWANMRLVAPAVLSWLLAKTLLLLFASSFAALTPEI
                     GAVLANTLSNLISAVLLIYLFRLYMLIRQ"
     gene            complement(18736..18825)
                     /locus_tag="J1Y83_001053"
                     /pseudo
     CDS             complement(18736..18825)
                     /locus_tag="J1Y83_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460692.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
     gene            19065..19718
                     /gene="ompW"
                     /locus_tag="J1Y83_001054"
     CDS             19065..19718
                     /gene="ompW"
                     /locus_tag="J1Y83_001054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein OmpW"
                     /protein_id="HBA7629223.1"
                     /translation="MKKLTVAALAVATLLSGSAFAHEAGEFFMRAGSATVRPTEGAGG
                     TLGSLGGFSVTNNTQLGLTFTYMATDNIGVELLAASPFRHKVGTATTGDIATVHHLPP
                     TLMAQWYFGDSGSKFRPYIGAGINYTTFFNNDFNDTGEGLGLSDLSLKDSWGVAGQVG
                     LDYLINRDWLLNMSVWYMDIDTEVKYKSNGAVLPAGRYSDNVRLDPWVFMFSAGYRF"
     gene            complement(19769..20575)
                     /gene="trpA"
                     /locus_tag="J1Y83_001055"
     CDS             complement(19769..20575)
                     /gene="trpA"
                     /locus_tag="J1Y83_001055"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000443083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HBA7629224.1"
                     /translation="MERYESLFAQLKERKEGAFVPFVTLGDPGIEQSLKIIDTLIEAG
                     ADALELGIPFSDPLADGPTIQNATLRAFAAGVTPAQCFEMLALIRQKHPTIPIGLLMY
                     ANLVFNKGIDEFYAECEKVGVDSVLVADVPVEESAPFRQAALRHNVAPIFICPPNADD
                     DLLRQIASYGRGYTYLLSRAGVTGAENRAALPLNHLVTKLKEYNAAPPLQGFGISAPD
                     QVKAAIDAGAAGAISGSAIVKIIEQHINEPEKMLAALKAFVQPMKAATRR"
     gene            complement(20575..21768)
                     /gene="trpB"
                     /locus_tag="J1Y83_001056"
     CDS             complement(20575..21768)
                     /gene="trpB"
                     /locus_tag="J1Y83_001056"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016158396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HBA7629225.1"
                     /translation="MTTLLNPYFGEFGGMYVPQILMPALRQLEEAFVSAQKDPEFQAQ
                     FNDLLKNYAGRPTALTKCQNITAGTNTTLYLKREDLLHGGAHKTNQVLGQALLAKRMG
                     KTEIIAETGAGQHGVASALASALLGLKCRIYMGAKDVERQSPNVFRMRLMGAEVIPVH
                     SGSATLKDACNEALRDWSGSYETAHYMLGTAAGPHPYPTIVREFQRMIGEETKAQILE
                     REGRLPDAVIACVGGGSNAIGMFADFINETDVGLIGVEPGGHGIETGEHGAPLKHGRV
                     GIYFGMKAPMMQTEDGQIEESYSISAGLDFPSVGPQHAYLNSTGRADYVSITDDEALE
                     AFKTLCLHEGIIPALESSHALAHALKMMRENPEKEQLLVVNLSGRGDKDIFTVHDILK
                     ARGEI"
     gene            complement(21780..23138)
                     /gene="trpCF"
                     /locus_tag="J1Y83_001057"
     CDS             complement(21780..23138)
                     /gene="trpCF"
                     /locus_tag="J1Y83_001057"
                     /EC_number="4.1.1.48"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309861.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional indole-3-glycerol-phosphate
                     synthase TrpC/phosphoribosylanthranilate isomerase TrpF"
                     /protein_id="HBA7629226.1"
                     /translation="MQTVLAKIVADKAIWVEARKQQQPLASFQNEIQPSTRHFYDALQ
                     GARTAFILECKKASPSKGVIRDDFDPARIAAIYKHYASAISVLTDEKYFQGSFDFLSI
                     VSQIAPQPILCKDFIIDPYQIYLARYYQADACLLMLSVLDDEQYRQLAAVAHSLEMGV
                     LTEVSNEEELERAIALGAKVVGINNRDLRDLSIDLNRTRELAPKLGHNVTVISESGIN
                     TYAQVRELSHFANGFLIGSALMAHDDLNAAVRRVLLGENKVCGLTRGQDAKAAYDAGA
                     IYGGLIFVATSPRCVNVEQAQEVMAAAPLQYVGVFRNHDIADVVDKAKVLSLAAVQLH
                     GNEDQLYIDTLRDALPENVAIWKALSVGETLPARELQHVDKYVLDNGQGGSGQRFDWS
                     LLNGQSLGNVLLAGGLGADNCVEAAQTGCAGLDFNSAVESQPGIKDARLLASVFQTLR
                     AY"
     gene            complement(23142..24737)
                     /gene="trpD"
                     /locus_tag="J1Y83_001058"
     CDS             complement(23142..24737)
                     /gene="trpD"
                     /locus_tag="J1Y83_001058"
                     /EC_number="2.4.2.18"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008784940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional anthranilate synthase glutamate
                     amidotransferase component TrpG/anthranilate
                     phosphoribosyltransferase TrpD"
                     /protein_id="HBA7629227.1"
                     /translation="MADILLLDNIDSFTYNLADQLRSNGHNVVIYRNHIPAQTLIERL
                     ATMSNPVLMLSPGPGVPSEAGCMPELLTRLRGKLPIIGICLGHQAIVEAYGGYVGQAG
                     EILHGKASSIEHDGQAMFAGLTNPLPVARYHSLVGSNIPAGLTINAHFNGMVMAVRHD
                     VDRVCGFQFHPESILTTQGARLLEQTLAWAQQKLEPTNTLQPILEKLYQAQTLSQQES
                     HQLFSAVVRGELKPEQLAAALVSMKIRGEHPNEIAGAATALLENAAPFPRPDYLFADI
                     VGTGGDGSNSINISTASAFVAAACGLKVAKHGNRSVSSKSGSSDLLAAFGINLDMNAD
                     KSRQALDELGVCFLFAPKYHTGFRHAMPVRQQLKTRTLFNVLGPLINPAHPPLALIGV
                     YSPELVLPIAETLRVLGYQRAAVVHSGGMDEVSLHAPTIVAELHDGEIKSYQLTAEDF
                     GLTPYHQEQLAGGTPEENRDILTRLLQGKGDAAHEAAVAANVAMLMRLHGHEDLQANA
                     QTVLEVLRSGSAYDRVTALAARG"
     gene            complement(24737..26299)
                     /gene="trpE"
                     /locus_tag="J1Y83_001059"
     CDS             complement(24737..26299)
                     /gene="trpE"
                     /locus_tag="J1Y83_001059"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HBA7629228.1"
                     /translation="MQTQKPTLELLTCKGAYRDNPTALFHQLCGDRPATLLLESADID
                     SKDDLKSLLLVDSALRITALSDTVTIQALSGNGEALLTLLDNALPAGVENEQSPNCRV
                     LCFPPVSPLLDEDARLCSLSVFDAFRLLQNLLNVPKEEREAMFFGGLFSYDLVAGFED
                     LPQLSAENNCPDFCFYLAETLMVIDHQKKSTRIQASLFAPNEEEKQRLTARLNELRQQ
                     LTEAAPPLPVVSVPHMRCECNQSDEEFGGVVRLLQKAIRAGEIFQVVPSRRFSLPCPS
                     PLAAYYVLKKSNPSPYMFFMQDNDFTLFGASPESSLKYDATSRQIEIYPIAGTRPRGR
                     RADGSLDRDLDSRIELEMRTDHKELSEHLMLVDLARNDLARICTPGSRYVADLTKVDR
                     YSYVMHLVSRVVGELRHDLDALHAYRACMNMGTLSGAPKVRAMQLIAEAEGRRRGSYG
                     GAVGYFTAHGDLDTCIVIRSALVENGIATVQAGAGVVLDSVPQSEADETRNKARAVLR
                     AIATAHHAQETF"
     gene            complement(26391..26435)
                     /gene="trpL"
                     /locus_tag="J1Y83_001060"
     CDS             complement(26391..26435)
                     /gene="trpL"
                     /locus_tag="J1Y83_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trp operon leader peptide"
                     /protein_id="HBA7629229.1"
                     /translation="MKAIFVLKGWWRTS"
     gene            26573..27454
                     /gene="yciV"
                     /locus_tag="J1Y83_001061"
     CDS             26573..27454
                     /gene="yciV"
                     /locus_tag="J1Y83_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-3' exoribonuclease"
                     /protein_id="HBA7629230.1"
                     /translation="MSDTNYAVIYDLHSHTTASDGCLTPEALVHRAVEMRVGTLAITD
                     HDTTAAIAPAREEISRSGLALNLIPGVEISTVWENHEIHIVGLNIDITHPLMCEFLAQ
                     QTERRNQRAQLIAERLEKAQIPGALEGAQRLAQGGAVTRGHFARFLVECGKASSMADV
                     FKKYLARGKTGYVPPQWCTIEQSIDVIHHSGGKAVLAHPGRYNLSAKWLKRLVAHFAE
                     HHGDAMEVAQCQQSPNERTQLATLARQHHLWASQGSDFHQPCPWIELGRKLWLPAGVE
                     GVWQLWEQPQNTTEREL"
     gene            27451..28071
                     /locus_tag="J1Y83_001062"
     CDS             27451..28071
                     /locus_tag="J1Y83_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonylcarbamoyl-AMP synthase"
                     /protein_id="HBA7629231.1"
                     /translation="MSQFFYIHPDNPQQRLINQAVEIVRKGGVIVYPTDSGYALGCKI
                     EDKNAMERICRIRQLPDGHNFTLMCRDLSELSTYSFVDNVAFRLMKNNTPGNYTFILK
                     GTKEVPRRLLQEKRKTIGMRVPSNPIAQALLEALGEPMLSTSLMLPGSEFTESDPEEI
                     KDRLEKQVDLIIHGGYLGQKPTTVIDLTDDTPVVVREGVGDVKPFL"
     gene            28099..29988
                     /locus_tag="J1Y83_001063"
     CDS             28099..29988
                     /locus_tag="J1Y83_001063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2207 domain-containing protein"
                     /protein_id="HBA7629232.1"
                     /translation="MAGIYRCILLLIVGLFFSSLSYAKNTEIPSYEEGISLFDVEATL
                     QPNGVLDIKENIHFQARNQQIKHGFYRDLPRLWMQPDGDAALLNYHIVGVTRDGIPEP
                     WHLDWHIGLMSIVVGDKQRFLPQGDYHYQIHYQVKNAFLREGDSDLLIWNVTGNHWPF
                     EIYKTLFSLKLPDIAGNPFSEIDLFTGEEGDTYRNGRILEDGRIESRDPFYREDFTVL
                     YRWPHALLGNAPAPQTTNIFSHLLLPSTSSLLICFPSLFLACGWLYLWKRRPQFTPVD
                     VIETDVIPPDYTPGMLRLDAKLVYDDKGFCADIVNLIVKGKIHLEDHYDKNQQILIRV
                     NEGATRNNAVLLPAEQLLLEALFRKGDKVVLTGRRNRVLRKAFLRMQKFYLPRKKSSF
                     YRPDAFLQWGGMAILAVILYGNLSPVGWAGMSLVGDMFIMICWLLPFLFCSLELLFAR
                     DDDKPCVNRVIITLFLPLICSGVAFYSLYINVGDVFFYWYMPAGYFSAVFLTGYLTGM
                     GYIFLPKFTQTGQQRYANGEAIVNDLARKEAATHSGRRRKGETRKLDYALLGWAISAN
                     LGREWALRIAPSLSSAICAPEIARNGVLFSLQTHLSCGAYTSLLGRSYSGGGSGAGGG
                     GGGGW"
     gene            30201..31076
                     /gene="rluB"
                     /locus_tag="J1Y83_001064"
     CDS             30201..31076
                     /gene="rluB"
                     /locus_tag="J1Y83_001064"
                     /EC_number="5.4.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2605) synthase RluB"
                     /protein_id="HBA7629233.1"
                     /translation="MSEKLQKVLARAGHGSRREIESIIEAGRVSVDGKIAKLGDRVEI
                     TPGLKIRIDGHLISVRESAEQICRVLAYYKPEGELCTRNDPEGRPTVFDRLPKLRGAR
                     WIAVGRLDVNTCGLLLFTTDGELANRLMHPSREVEREYAVRVFGQVDDAKLRDLSRGV
                     QLEDGPAAFKTIKFSGGEGINQWYNVTLTEGRNREVRRLWEAVGVQVSRLIRVRYGDI
                     PLPKGLPRGGWTELDLAQTNYLRELVELPPETSSKVAVEKDRRRMKANQIRRAVKRHS
                     QVSGGRRSGGRNNNG"
     gene            complement(31116..31706)
                     /gene="cobO"
                     /locus_tag="J1Y83_001065"
     CDS             complement(31116..31706)
                     /gene="cobO"
                     /locus_tag="J1Y83_001065"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HBA7629234.1"
                     /translation="MSDERYQQRQQRVKEKVDARVAQAQDERGIIIVFTGNGKGKTTA
                     AFGTATRAVGHGKKVGVVQFIKGTWPNGERNLLEPHGVEFQVMATGFTWDTQNRESDT
                     AACREVWQHAKRMLADSSLDMVLLDELTYMVAYDYLPLEEVMQALNERPHQQTVIITG
                     RGCHRDILELADTVSELRPVKHAFDAGVKAQIGIDY"
     gene            complement(31703..32461)
                     /locus_tag="J1Y83_001066"
     CDS             complement(31703..32461)
                     /locus_tag="J1Y83_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciK family oxidoreductase"
                     /protein_id="HBA7629235.1"
                     /translation="MHYQPKQDLLNDRIILVTGASDGIGREAAMTYARYGATVILLGR
                     NEEKLRQVASHINEETGRQPQWFILDLLTCTSEDCQQLAQRIAVNYPRLDGVLHNAGL
                     LGDVCPMSEQDPQVWQDVMQVNVNATFMLTQALLPLLLKSDAGSLVFTSSSVGRQGRA
                     NWGAYAASKFATEGMMQVLADEYQQHLRVNCINPGGTRTAMRASAFPTEDPQKLKTPA
                     DIMPLYLWLMGDDSRRKTGMTFDAQPGRKPGISQ"
     gene            32681..33730
                     /gene="sohB"
                     /locus_tag="J1Y83_001067"
     CDS             32681..33730
                     /gene="sohB"
                     /locus_tag="J1Y83_001067"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease SohB"
                     /protein_id="HBA7629236.1"
                     /translation="MELLSEYGLFLAKIVTVVLAIAAIAAIIVNVAQRNKRQRGELRV
                     NNLSEQYKEMKEELAAALMDTHQQKQWHKAQKKKHKQEAKAAKAKAKLGEVVTDSKPR
                     VWVLDFKGSMDAHEVNSLREEITAVLAAFKPQDQVVLRLESPGGMVHGYGLAASQLQR
                     LRDKNIPLTVTVDKVAASGGYMMACVADKIVSAPFAIVGSIGVVAQMPNFNRFLKSKD
                     IDIELHTAGQYKRTLTLLGENTEEGREKFREELNETHQLFKDFVKRMRPSLDIEQVAT
                     GEHWYGQQAVEKGLVDEINTSDEVILSLMEGREVVNVRYMQRKRLIDRFTGSAAESAD
                     RLLLRWWQRGQKPLM"
     gene            complement(33766..34017)
                     /locus_tag="J1Y83_001068"
     CDS             complement(33766..34017)
                     /locus_tag="J1Y83_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciN family protein"
                     /protein_id="HBA7629237.1"
                     /translation="MNKETQPIDRETLLKEANKIIREHEDTLAGIEATGVTQRNGVLV
                     FTGDYFLDEQGLPTAKSTAVFNMFKHLAHVLSEKYHLVD"
     gene            34397..36994
                     /gene="topA"
                     /locus_tag="J1Y83_001069"
     CDS             34397..36994
                     /gene="topA"
                     /locus_tag="J1Y83_001069"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000513601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HBA7629238.1"
                     /translation="MGKALVIVESPAKAKTINKYLGSDYVVKSSVGHIRDLPTSGSAA
                     KKSADSTSTKTAKKPKKDERGALVNRMGVDPWHNWEAHYEVLPGKEKVVSELKQLAEK
                     ADHIYLATDLDREGEAIAWHLREVIGGDDARYSRVVFNEITKNAIRQAFNKPGELNID
                     RVNAQQARRFMDRVVGYMVSPLLWKKIARGLSAGRVQSVAVRLVVEREREIKAFVPEE
                     FWEVDASTTTPSGEALALQVTHQNDKPFRPVNKEQTQAAVSLLEKARYSVLEREDKPT
                     TSKPGAPFITSTLQQAASTRLGFGVKKTMMMAQRLYEAGYITYMRTDSTNLSQDAVNM
                     VRGYISDNFGKKYLPESPNQYASKENSQEAHEAIRPSDVNVMAESLKDMEADAQKLYQ
                     LIWRQFVACQMTPAKYDSTTLTVGAGDFRLKARGRILRFDGWTNVMPALRKGDEDRIL
                     PAVDKGDALTLVELTPAQHFTKPPARFSEASLVKELEKRGIGRPSTYASIISTIQDRG
                     YVRVENRRFYAEKMGEIVTDRLEENFRELMNYDFTAQMENSLDQVANHEAEWKAVLDH
                     FFSDFTQQLDKAEKDPEEGGMRPNQMVLTSIDCPTCGRKMGIRTASTGVFLGCSGYAL
                     PPKERCKTTINLVPENEVLNVLEGEDAETNALRAKRRCPKCGTAMDSYLIDPKRKLHV
                     CGNNPTCDGYEIEEGEFRIKGYDGPIVECEKCGSEMHLKMGRFGKYMACTNEECKNTR
                     KILRNGEVAPPKEDPVPLPELPCEKSDAYFVLRDGAAGVFLAANTFPKSRETRAPLVE
                     ELYRFRDRLPEKLRYLADAPQQDPEGNKTMVRFSRKTKQQYVSSEKDGKATGWSAFYV
                     DGKWVEGKK"
BASE COUNT         9313 a         8942 c         9518 g         9249 t
ORIGIN      
        1 cacacccgat ggaactgtaa atatcacata gtttttgcgc caaaataccg aagacaagtg
       61 ttctacagag agaagcgtag agcaataggc agtattttga gaaagctgtg tgagtggaaa
      121 agcgtacgga ttcttgaagc tgaatgctgt gcagatcata tccatatgct tgtggagatc
      181 ccgcccaaaa tgagcgtatc cggctttatg ggatatctga aagggaaaag cagtctgatg
      241 ctttacgagc agtttggtga tttgaaattc aaatacagga acagagagtt ctggtgcaga
      301 gggtactacg tcgatacggt gggtaagaac acggcgaaga tacaggatta cataaagcac
      361 cagcttgaag aggataaaat gggagagcag ttatcgattc cctatccggg cagcccgttt
      421 acgggccgta agtaacgaag ttggatgcaa atgtcagatc gtgtgcgcct gttagggcgc
      481 ggctggtaag agagccttat aggcgcattt gaaaaacctc cggctatgcc ggaggatatt
      541 tattcggggc tgtggggtat ttccagggcc tctcctttac cactctcggg aaggcccttt
      601 cccttcttgt cggtaatttc actggttgtc ccatacttgc aagatcgcgc atcagctccg
      661 gtatacgtcc tggggaagcg tatggcgtcc gtcatcgacg tcagcagatg gcagactttt
      721 cctttgcgcg tcacggtcag caggcgggct gtcacctcat ttcccagtca cggccacttt
      781 tttcgtgcct gcgggctggt tttcagcttc accagatgat cgcctttacc cagttttcta
      841 atctcttcat attgcgctcc ctttctgaga gggatcatcc agtggcggtg ttctcccgcc
      901 aggctccagg catttaacag tcctggtgag taataacctt tatccattaa cgtcagtgtt
      961 atcgccggtt tgttctataa gttgctcagc aagctcattt tcgctgttct tcatcgtgcc
     1021 gaaggctgca gccgtcagca gatggctggt cagttccatc tggcagacca ttttgacctg
     1081 cgggtagagc gccgggttcc cggcatgtgt ctggcggggg aaggctgcat cgttctctgg
     1141 tgtatccggt gtgcgccaga acacaccatc gatggccagc agggtcaggc cgcaccagtg
     1201 cggatgcgga gtggcgttat gccagagctg cgctgttttc gtgaacacgc tgcggacagc
     1261 ctcaattccc agacgctggc gggtctgaat aacggcactg ggggcaacga aggggcgatt
     1321 gcccggcagc atgatgtcca ggcgattcgc aatctggtga agaggttctt tacgctcaag
     1381 cgccatgcca acaatacacc agaccatcat ttcgagggga agacggcgct tgtgtagcgt
     1441 tacagtacct gattcggcaa ggcaacgaga gatgagttcg ggggcgaggt aatcccccag
     1501 agaagtcagt gggttacgca gagaatcgta acgggatatc aaatcaaggg cctgtccaat
     1561 gagcataaaa aaatccggaa acgagtgagc gtttccggat tcttacacag ccactggatc
     1621 ggtcaactga tccttaactg actggcatta ccccttatgg ggccgtttat gttgccagac
     1681 agcgctactg attaagcgga tttcttcgct tttttctcag ctttagccgg agcggcttct
     1741 tttttcgctg cagtttcacc ttctacataa tcacgaccgt agtaggtatc cagcagaatc
     1801 tgtttcagct cggagatcag tgggtaacgc gggttagcgc cggtgcactg gtcatcgaat
     1861 gcatcttcag acagtttatc cacattcgcc aggaagtctg cttcctgaac gccagcttca
     1921 cggatagatt tcggaatacc cagttcagcc ttcagcgttt ccagccatgc cagcagtttc
     1981 tcgatcttag cagcagtacg gtcgcccggt gcgctcagac ccaggtggtc ggcaatttca
     2041 gcataacgac ggcgagcctg cggacggtca tactggctga atgcagtctg cttggtcggg
     2101 ttgtcattcg cgttgtagcg aataacgtta caaatcagca gggcgtttgc cagaccgtgc
     2161 ggaatatgga actgggaacc cagtttgtgc gccattgagt gacatacacc caggaaggcg
     2221 ttcgcaaacg cgatacccgc gatagtcgct gcactgtgaa cacgttcacg cgctaccgga
     2281 tttttagacc cttcgtggta ggacgctggc agatattctt tcagtagttt cagtgcctgc
     2341 agagcctgac catcagagaa ctcagatgcc agtacagaaa cataagcttc catggcgtga
     2401 gttactgcgt ccagaccacc gaaagcacac agggacttcg gcatgtccat aacaaggttg
     2461 gcgtcgacaa tcgccatatc cggggtcagt gcatagtctg ccagcggata tttctgacca
     2521 gtagtgtcgt cagttacaac cgcaaacgga gtgacttcag aacctgtacc agaagtggtg
     2581 gtgacagcga tcattttcgc tttcacaccc attttcggga acttgtagat acgtttacgg
     2641 atatccataa agcgcagcgc cagttcttcg aagtgagttt ccggatgttc gtacataacc
     2701 cacatgatct tcgcagcgtc catcggggaa ccaccaccca gcgcgataat cacgtctggt
     2761 ttgaaggagt ttgccagttc tgcaccttta cgaacgatgc tcagggtcgg gtcagcttct
     2821 acttcgaaga agacttcagt ttcaacgcct gctgctttca atacggaagt gatctgatca
     2881 gcataaccat tgttgaacag gaagcggtca gtcacgatga gcgcacgttt gtggccatca
     2941 gtaatcactt catctagcgc gattggcagg gagccacggc ggaagtagat agatttcgga
     3001 agtttgtgcc acaacatgtt ttcagctcgc ttagcaacgg ttttcttgtt gatcaggtgt
     3061 ttcggaccaa cgttttcaga gatggagtta ccaccccaag aaccacaacc cagagtcagg
     3121 gaaggtgcga gtttgaagtt atacaggtca ccgataccac cctgagaagc tggggtgtta
     3181 atcaggatac gagccgtttt cattttctga ccgaagtaag aaacgcgagc cggttggtta
     3241 tcctggtcag tgtacaggca agaggtatga ccgataccgc ccatagcaac cagtttctct
     3301 gctttttcta ccgcgtcttc gaaatcttta gcacggtaca ttgccagagt cggggacagt
     3361 ttttcatgtg cgaacggttc gctttcatca acaacggtca cttcaccgat cagaatcttg
     3421 gtgttttctg gtacagagaa gcctgccagt tcagcaattt tataggctgg ctgaccaacg
     3481 atagccgcgt tcagcgcacc gtttttcagg ataacgtcct gaacagcttt cagctcttta
     3541 ccctgcaaca gatagccgcc gtgggttgcg aaacgttcac gtactgcgtc ataaacagag
     3601 tcaacaacaa caacagactg ttcagaagca cagattacgc cgttgtcgaa ggtttttgac
     3661 atcagtacag atgcaactgc acgtttgata tcagcagttt cgtcaataac aactggagtg
     3721 ttacccgcgc ctacaccgat agctggttta ccggagctgt atgcggcttt aaccatgccc
     3781 ggaccaccag tcgcgaggat caggttgatg tctgggtggt gcatcagtgc gttagacagt
     3841 tcaacagaag gttgatcgat ccagccgatc agatctttcg gagcaccggc agcgatagca
     3901 gcctgtagaa cgatatcagc cgctttgttg gttgcatctt ttgcacgcgg gtgcggggag
     3961 aagataatgg cgttacgggt cttcaggctg atcagcgatt tgaagatagc agttgaagtc
     4021 gggttagtgg tcggaacgat accgcaaata ataccgattg gttcagcgat agtgatggta
     4081 ccaaaagtgt cgtcttcaga cagaacacca caggtttttt catctttata agcgttgtag
     4141 atatattcag aagcaaagtg gtttttgatc actttatctt cgacgatacc catgccggat
     4201 tcggcaacgg ccattttcgc gagtgggatt cgagcatctg cagcagccag agcggcggcg
     4261 cggaagattt tgtctacttg ctcttgagtg aaactggcat attcacgctg ggcttttttt
     4321 acacgctcta cgagtgcgtt aagttcagcg acattagtaa cagccataat gctctcctga
     4381 taatgttaaa cttttttagt aaatcatctg ctcgaatacg agagtatagt cagtgcggtg
     4441 atgatttgct taaccgatgg aaatcaaaag cttactcgcg ctcacactca ctgtgattta
     4501 ctaaaagagt ttaaacatta gagttattat ctctaatgcg tcacttccag gtggcgtaag
     4561 caagattact cacttctggg tactgattac gtgatccaaa tcaaaatttt gcaaagctga
     4621 cacctttcag catggctttt cgccattata gctaacagtt aataaattgt agtatgattt
     4681 gttggctaca ttagcatgtt ttgcacaact agataacaat aacgaatgat agcaatttta
     4741 agtagttagg aggtgaaaaa tgctgtcaaa aggcgtattg tcagcgcgtc ttttcaacct
     4801 tatttatggc taacattatc cggcttttgc ttcggagcta accgtgattc agaccctttt
     4861 tgattttccc gtttacttca aatttttcat cgggttattt gcgctggtca acccggtagg
     4921 gattattccc gtctttatca gcatgaccag ttatcagaca gcggcagcgc gaaacaaaac
     4981 taaccttaca gccaacctgt ctgtggccat tatcttgtgg atctcgcttt ttctcggcga
     5041 cacgattcta caactttttg gtatatcaat tgattcattc cgtatcgccg gtggcatcct
     5101 ggtggtgaca atagcgatgt cgatgatcag cggcaagctt ggtgaggata aacagaacaa
     5161 gcaagaaaaa tcagaaaccg cggtacgtga aagcattggt gtggtgccat tggcgttgcc
     5221 gttgatggcg gggccagggg cgatcagttc taccatcgtc tggggtacgc gttatcacag
     5281 cattagttat ctgtttggtt tctttgtggc tattgcattg ttcgctttat gttgctgggg
     5341 attgttccgc atggcaccgt ggctggtacg agttttacgc cagaccggca tcaacgtgat
     5401 tacgcgtatt atggggctat tgctgatggc attggggatt gaatttatcg ttactggtat
     5461 taaggggatt ttccccggcc tgcttaatta attcctttca aatgaaacgg agctgccatg
     5521 ctccgtttac ttcgtcatta tttttacttt gttcctgcgc agttatcaaa agcaaaagga
     5581 ataggtaaaa atattcttct caaattacag ttagttataa ggatttcctt aactgcttct
     5641 cctcaccatc atgttatttt cgccacatca taatcctggg cttgctgaag aataattgaa
     5701 atgatattag taattccact gcctttggta gaggaaagtg ctaaataata atcaattgtt
     5761 aaattattgt gcatttctct actggaactg taatcagaaa agagagacat gcttaaccaa
     5821 cctctatttg attgaattga aagatgtttg ttaaggcatg gatgcaagct atagattctg
     5881 gtacggtcaa taaaagagaa ttgcttaaca attttgcaaa atgtattggc gagtaagaac
     5941 cgcatttggt actttccggg caaccgccag gcgattcttt attggtaatg agaataatta
     6001 acaattaaag atcgtcgcga aagaataatg tgtctcgaca ggggagacac agtacgaatc
     6061 gacataaggt gatcgtctga atcaccagaa taaataaagt cggtgatagt aatacgtaac
     6121 gataaagtaa cctgacagca gaaagtctcc gagcctgtgc agggtcccaa tccgggatta
     6181 cacatgctgg ttaataccag taattataat gagggagtcc aaaaaacaat gaccaacatc
     6241 accaagagaa gtttagtagc agctggcgtt ttggctgcgc ttatggcagg gaatgtcgcg
     6301 ctggcagctg atgtacccgc aggtgtcaca ctggcggaaa aacaaacgct ggtacgtaac
     6361 aatggttcag aagttcagtc attagatccg cacaaaattg aaggtgttcc ggagtctaat
     6421 atcagccgag acctgtttga aggcttactg gtcagcgatc ttgacggtca tccggcacct
     6481 ggcgtcgctg aatcctggga taataaagac gcgaaagtct ggaccttcca tttgcgtaaa
     6541 gatgcgaaat ggtctgatgg cacgccagtt acagcacaag actttgtgta tagctggcaa
     6601 cgttctgttg atccaaacac tgcttctccg tatgccagtt atctgcaata tgggcatatc
     6661 gccggtattg atgaaattct tgaagggaaa aaaccgatta ccgatctcgg cgtgaaagct
     6721 attgatgatc acacattaga agtcacctta agtgaacctg ttccgtactt ctataaatta
     6781 cttgttcacc catcaacttc accggtgcca aaagccgcta tcgagaaatt cggcgaaaaa
     6841 tggacccagc ctggtaatat cgtcaccaac ggtgcctata ccttaaaaga ttgggtcgta
     6901 aacgaacgaa tcgttcttga acgcagcccg acctactgga acaacgcgaa aaccgttatt
     6961 aaccaggtaa cctatttacc tattgcttct gaagttaccg atgtcaaccg ctaccgtagt
     7021 ggtgaaatcg acatgactta taacaacatg ccgatcgaat tgttccagaa gctgaaaaaa
     7081 gagatcccgg acgaagttca cgttgatcca tacctgtgca cttactatta cgaaattaac
     7141 aaccagaaac cgccatttaa tgacgtgcgt gtgcgtaccg cactgaaatt gggtatggac
     7201 cgcgatatca ttgttaataa agtgaaagcg cagggcgaca tgcccgccta tggttacact
     7261 ccaccatata ctgatggcgc aaaattgact cagccggaat ggtttggctg gagccaggaa
     7321 aaacgtaacg aagaagcgaa aaaactgctg gctgaagcgg gttataccgc agacaaaccg
     7381 ttgaccatca acctgttgta taacacctcc gatctgcata aaaagctggc gattgctgcc
     7441 tcttcattgt ggaagaaaaa cattggtgta aacgtcaaac tggttaacca ggagtggaaa
     7501 acgttcctcg acacccgtca ccagggcact tttgatgtgg cccgtgcagg ctggtgtgca
     7561 gactacaacg aaccaacttc cttcctgaac accatgctgt cgaacagctc aatgaatacc
     7621 gcgcattata agagcccggc ctttgacagc attatggcgg aaacgctgaa agtgactgac
     7681 gaggcgcagc gtacagctct gtacactaaa gcagaacaac agctggataa ggattcggcc
     7741 attgttcctg tttattacta cgtgaatgcg cgtttggtga aaccgtgggt tggtggctat
     7801 accggcaaag atccgctgga taatacctac acccggaata tgtacattgt gaagcactaa
     7861 tggcaatacg tggggcagga gtgtcctgct ccacggtgtc tgatttttat cgcattacag
     7921 aaggcacagg ccagaaggta gggcaatgtt aaaatttatt ctacgtcgct gtctggaagc
     7981 gattccgacg ctatttattc ttattactat ttcgttcttt atgatgcgcc tcgctccggg
     8041 cagtcctttt accggcgaac gtactttacc accggaagtg atggccaata tcgaagcgaa
     8101 atatcatctt aatgatccga tcatgacaca gtatttcagc tacctgaaac aactggcgca
     8161 tggcgatttc ggtccatcgt ttaaatataa agattattcg gtcaatgacc tggttgcatc
     8221 cagttttccc gtttctgcca aactgggagc cgcagcattt tttcttgcgg taatactggg
     8281 tgttagtgct ggcgttattg ccgcattaaa gcaaaacacc aaatgggact ataccgtgat
     8341 ggggctggca atgaccgggg ttgttatccc cagttttgtg gttgcgccat tattagtcat
     8401 gatatttgcg atcattttgc attggctgcc gggcggtggc tggaatggtg gagcgcttaa
     8461 attcatgata ttgccaatgg tggcgttatc actcgcctat atcgccagta ttgcgcgtat
     8521 tacccgtggc tctatgattg aggttttaca ctccaacttt attcgtactg cccgggcgaa
     8581 agggttacct atgcggcgga tcattttacg ccacgcatta aaacctgctc tgttacccgt
     8641 gctctcctat atggggcccg catttgtcgg cattattacc ggttcgatgg tcatcgaaac
     8701 catttatggt ttaccgggga ttgggcaatt gttcgttaat ggcgcattga accgtgacta
     8761 ttccttagtg ttaagtctga ccattctggt tggcgcgtta accattttgt ttaatgccat
     8821 tgtcgatgtg ctatatgcgg ttatcgaccc gaaaatccgt tactgatact ggagctcgcg
     8881 atgatgttaa gtaagaaaaa cagcgagacg ctggaaaatt tcagtgaaaa gctggaggtc
     8941 gaagggcgca gcttgtggca ggacgcacgt cgacgtttta tgcataaccg tgcggcggtt
     9001 gccagtctga tagtgctggt gctgatcgcg ttatttgtaa tcctggcacc gatgctttcg
     9061 cagtttgcct atgacgatac tgactgggcg atgatgtcca gcgccccgga tatggagtcc
     9121 ggtcactact ttggtactga ctcatccggt cgcgacctgc ttgtgcgcgt tgcgattggc
     9181 gggcgtatct cactcatggt cggtgttgct gcggcactgg tggcagtggt cgtggggaca
     9241 ctttatggtt cgctttccgg ttatctgggc ggtaaagtgg attcggtaat gatgcgtctg
     9301 ctggaaatcc tcaactcctt cccattcatg ttcttcgtca ttttgctggt gacctttttc
     9361 ggtcaaaaca tcctgctgat tttcgtggcg attggcatgg tttcctggct ggatatggct
     9421 cgtattgtgc gtgggcaaac cctgagtctg aagcgcaaag agtttattga ggcggcacaa
     9481 gttggcggtg tatcgacgcc gggcattgtt attcgccaca ttgtgccgaa cgtactcggt
     9541 gtggtggtgg tctatgcgtc gcttctggtg cccagcatga tcctctttga atctttcctt
     9601 agcttcctgg gattaggtac gcaagagccg ttaagcagct ggggtgcatt gctgagtgat
     9661 ggcgcgaact cgatggaagt ctctccatgg ttactgttgt tccctgcggg attcctcgtg
     9721 gtaacgctgt tttgtttcaa ctttatcggc gatggcttgc gtgatgccct cgacccgaaa
     9781 gatcgttaag gagtgcagcc atgagcgtaa ttgaaactgc aactgtgccg ctcgcacaac
     9841 aacaggctga cgcactgctg aacgtgaaag atttgcgtgt cacctttagt accccggatg
     9901 gcgacgtcac ggcggtcaat gatttgaatt tttcgctacg tgccggagaa acgctgggca
     9961 tcgtaggtga gtctggttcg ggcaaatcgc aaaccgcatt tgcgttgatg ggcctgttgg
    10021 ctgccaacgg acgtattggc ggatcggcaa ccttcaatgg gcgtgaaatc ctcaatttgc
    10081 cagagcgtga actcaataaa ctgcgagctg aacaaatctc aatgattttt caggacccaa
    10141 tgacttcgtt gaatccctat atgcgcgtcg gtgagcagtt gatggaagtg ctgatgctgc
    10201 ataagaacat gagcaaagct gaggcgtttg aagaatcggt gcggatgctc gatgcggtaa
    10261 aaatgccgga agcgcgtaaa cgcatgaaaa tgtacccgca cgaattttct ggcggcatgc
    10321 gtcagcgagt catgattgcg atggcattgc tatgtcgacc taagctgctg attgcggatg
    10381 aacccactac tgcgctggac gtcaccgtac aggcgcagat catgacgcta ttgaatgaac
    10441 tgaagcggga atttaatacc gccatcatta tgattaccca cgatcttggc gtggtggcgg
    10501 ggatctgcga caaagtgctg gtaatgtacg ccgggcgcac gatggaatat ggcaacgcgc
    10561 gcgatgtctt ttatcaaccc gtacatcctt attctatcgg tttgctcaac gcggtgccgc
    10621 gtctcgatgc ggaaggtgaa acaatgttga ctattccggg taatccgcca aacctgctgc
    10681 gattaccgaa aggttgcccg ttccagccac gttgtccgca tgcgatggaa atttgtagta
    10741 gcgcaccgcc gctggaagag tttacgcctg gccgtctgcg tgcttgcttt aaaccagtgg
    10801 aggaactgtt atgaatgctg taaccgaagg aagaaaagtc ctcctcgaaa tcgccgatct
    10861 gaaagtgcac tttgaaatca aagatggcaa acagtggttc tggcaaccgc cgaaaacgct
    10921 caaagccgtc gatggtgtaa ctcttcgcct gtatgaaggg gaaacattag gtgtggtagg
    10981 ggaatcggga tgcgggaagt ccacctttgc tcgcgccatc attggcttgg ttaaagcgac
    11041 cgacggtcat gtggcctggt taggtaaaga gttgctgggc atgaagcccg atgaatggcg
    11101 tgccgtacgc agtgatattc agatgatttt ccaggatccg ttggcatcac tgaacccgcg
    11161 tatgaccatc ggcgagatca tcgctgaacc actgcgtact tatcatccga aaatgtcacg
    11221 ccaggaagtt cgcgagcgcg tgaaggcgat gatgctgaaa gtcggtttgt tgcctaacct
    11281 gattaaccgc tatccgcatg agttctccgg tgggcagtgc cagcgtatcg ggattgctcg
    11341 tgctcttatt cttgaaccga agctgattat ctgtgatgag ccggtgtcgg cgctggacgt
    11401 gtcaattcag gcgcaggtgg tcaacctgct ccagcagttg caacgtgaga tgggattgtc
    11461 attaattttt atcgctcacg acctggccgt ggtaaaacac atttccgatc gtgtgctggt
    11521 gatgtatctc ggccatgcgg tagaactggg gacctatgat gaggtctacc acaatccgct
    11581 acatccttac actagggcat tgatgtcggc agtccccata cctgatccgg atctggagaa
    11641 gaacaaaacc atccagttac tggaagggga attaccgtcg ccgatcaacc cgccttccgg
    11701 ttgtgttttc cgtacccgtt gtccgattgc cggtccggag tgcgccaaaa cacgtcctgt
    11761 gctggaaggg agtttcagac acgccgtttc ttgcctgaaa gtcgatccgc tttaaaagca
    11821 ataagggctg acagttatca gccctttttc acgccaaaag cgattattta ttcccgccag
    11881 atgatatggc aaagtttgtg atctttttcg cggcataaca gaatgcgcgc gaaaacatcg
    11941 ttgatttcac catcttcact gtccgccaga ccaatcacca cttcggcaaa aaagtccggg
    12001 ttcaaatcga aatcaacatg ttcctgccag tcttccgccg gatcgaataa ctccgcgccg
    12061 ccgcgctctt caaactgaag attgaacagc agaacatcag ctggatcgag attgtcggca
    12121 gccagttcga gaaaaatatc gtaggcctgc tcaagcgttt catcttcagt caggcgattg
    12181 tttagatcca tatccatagt cactacctgt ttaacctctg ttggcgacgt tttacagcaa
    12241 cgggctgaag aagtaaaaca gtcgctcggc gacacgttgc cataatggac gttttagcca
    12301 taaacgggca tcgagcagac gagaacgcga aatataatcg tcctgaacgg cggcgaggtc
    12361 tgcaccaaaa cctttatcgt cgattgccag ggtaatctcg aaatttagcc acagactacg
    12421 catatcaagg ttaacggtgc caaccagact tagttcacca tcgaccagca cgctcttggt
    12481 atgcagtaac ccgccttcaa actgataaat tttaacccca gcagccagca gttctgtaaa
    12541 gaatgcgcga ctggcccagc cgaccagcat cgagtcattt tttcgcggaa ggataatact
    12601 gacatccacc ccgcgctgcg ccgccgtgca aatcgcatga agtaaatcat cgcttggcac
    12661 aaaatagggc gttgtcatga tcaaatattc acgagccgaa taagcagcag tcaataatgc
    12721 ctggtgaatg agatcttccg gaaagccggg gccagaagca attgtgtgaa tagtgtgacc
    12781 gctggcctgc tcaaacggca taatattgac atctggtggc ggcggcagaa tacgtttccc
    12841 ggtttcaatc tcccagtcgc aggaataaat aatccccatc gcggtggcga tggggccttc
    12901 catacgcgcc atcagatcaa tccattgccc tacgcccgca tcttgtttga agtagcgagg
    12961 gtcgaccata ttcatgctgc cggtgtacgc gatgtaatta tcaatcatga tcatcttgcg
    13021 atgttggcgc aggtccatac ggcgtaaaaa cacacgcatc agattgacct ttaaggcttc
    13081 gacaacttca ataccagcat tacgcattag ctcgggccac gggctgcgga aaaaagccac
    13141 actcccggcg gagtcgagca tcaatcggca atgaataccg cgccgtgcag cggccattaa
    13201 tgattcagcc acctggtccg ccatgccgcc gggctgccag atataaaaca ccatctcaat
    13261 attatggcgc gcgagctgga tgtcgcggat taacgcctgc atcacatcat ctgactcggt
    13321 catcagttgt agctgattcc ctttgacccc agcgatcccc tgacgacgct cgcaaagctt
    13381 gaataatggc gcagcgacac tgctattttc ttcggcgaag atatgcttac aggctttaag
    13441 gtcgttaagc cattttgcgg tggaaggcca catcgctctg gcgcgctcag cgcggcgttt
    13501 gcctaaatgg agctcgccaa cggcaagata ggcaataatt ccgactaacg gcagaatgta
    13561 aataatcaac agccaggcca tcgcggaggg aactgcgcgt cgtttcatta gaatgcgtaa
    13621 agttacgcct gcaatgagca accagtatcc cagaatggcc aaccaactca ccaacgtata
    13681 aacggttgtc atagattaga aatccttttg aaagcgcatt gttatgagtt tacgcatatg
    13741 gattcatctg gcaaataaaa acgcgggaaa agcactggtc agcgtcaggg gcgggggtat
    13801 aatgaccatt ctgttgttgc atagagtagt taacatgaag cggagtagaa cggaagtggg
    13861 gcgctggcgc atgcagcgtc aggctagccg acgtaaatcg cgttggcttg aggggcagtc
    13921 gcgccgaaat atgcgtatcc acagcatcag gaagtgcatt ctaaacaaac agcgtaactc
    13981 gttattgttt gcgatctaca atatctaaat gtactgggca ccattgcggt gcccagtaag
    14041 tttactcttc cgtctttaaa ctctattttt gcgccgattc tttactgtca gctttcgttt
    14101 cttgttcatc gttatcgctg aaaatgccat gaccggagtt caacaacatt gaaacgagca
    14161 tatcattatt aatctcttca ccatttaaaa ctcgcgccag aatttcgctg ccaaacagtt
    14221 gtggtgagag aatgatatcc ggatgtacca tcttaatctt attcaggttt ttgctatcac
    14281 tgacggcgag aactgttttg acatcactgc tcatatcttt tgccgagagt acaacaaacg
    14341 cgttatctgc atcgttatca ctcagcgcca gaatggcccg gcaacgatcg attcccgctt
    14401 tctttaacac tgaactgtca ttactgtcac cggggataac atcggcgtta tcgcctaagc
    14461 gttgctcaag ttgcttgata tcatcttcag gcaagttgct gataaccgtt acgttttgtc
    14521 cgcgttgatt cagttgcaga atcgtattga tggcgagaat cgaatgtccg caaacaataa
    14581 aatgatcttt acgatgcatt gtatgattgt ttccttttac aagtttgttg aatcccccgc
    14641 ggataagcgg accaaaaatt gaggtcatag atgtggcaaa aacggtaatg ccggaaataa
    14701 tgaccgaaat agtgaacaat cgtgctgatt cagaaacagg gacaatatcg ccatagccga
    14761 cggttgacat ggtttctatc gaaaaataga acgcggtcat caaactttct attcgcggat
    14821 taaaaccttc gcttaaataa agcgcaccgt aagtggagta aaacagtaac gtcgtgaaac
    14881 taataaatgc gaaaattgtc ccggctgctg cgctactgtg ggagaagtct ttgcgcagta
    14941 tcagcaaaaa caccagcgta aaaatgcaaa atccaatact aaatttcagc cagggataaa
    15001 aatgcagagt gtaaattagc gctatcaaca acaaaatgat actgattgcc catgcgagct
    15061 ttgcccggaa cagtaagcca atggagttca ggacaagaaa tacgccgagc ataaatagtg
    15121 gggcgtttgc cagcaaggag agatccagct ctgaaaatgc tttgatatgg aaaatatcaa
    15181 gcaaattgac cgacatacca tagattgttt taaaaataag caatccattt aaaaagacgg
    15241 ccagcgcgag aatgtcgtgc cgtagggtca cccataactt tgttgctgtt tgtttgaatg
    15301 tagtccagtg actcactgat acctctgtaa ccacaatttt gtttaattat ctcaacgtgc
    15361 acattttctt aacatacatg cagcgcgtga atgtgttaaa caatagtaga tgactttatc
    15421 cgcagtaatc aaaggttttg agtgagcaac tctgcgtcag gaaagggcat tttcctgaat
    15481 atgttcacat catataaaga aatacagata aagttattat ctgcttgtgg tggtgaatgc
    15541 actgaccgac ctgtaaggaa aggccaaaca agaacacggt tgcaaaaacc gtgcccttaa
    15601 atattgaatt tctattcaga acactttctt aaatggtttc actgaaacgt gttcatagac
    15661 tcctgccgcc acgtacgggt cagcatcggc ccaggcctga gctgcttcca gcgactcaaa
    15721 ttcagcaata acggttgagc cagtaaatcc cgcagcccct ggatcgttac tgtctaccgc
    15781 tggcattgga ccggcggtca acaaacgacc ttcatcatgc agtaactgta aacgtgctaa
    15841 atgtgccgga cgaacggaaa ggcgtttttc gaggctatca gctttgtctt gagcgtaaat
    15901 aacatacaac acaggcacaa ctccttgttc gggaaagttg taaagtacgt tatttgaaag
    15961 ggcgaagatc tgcaacggaa agatgatgtc tttgttaaga ccatgcataa agtaaggtca
    16021 attacgccaa aaatgacatt ttcactgatc ctgatcgtct tgccttattg aatatgattg
    16081 ctatttgcat ttaaaatcgg ggcctggttt ttctactgaa atgattatga cttcaatgac
    16141 ccttgattta cctcgccgct tcccatggcc gacgttactt tcggtctgca ttcatggtgc
    16201 tgttgtggcg ggtctgctct atacctcggt acatcaggtt attgaactac ctgcgcctgc
    16261 gcagccgatt tctgtcacga tggttgcgcc tgctgatctt gaaccgccac aagccgttca
    16321 gccaccaccg gagccggtgg tagagccaga accggaacct gagccgatcc ctgaaccgcc
    16381 aaaagaagct ccggtggtca ttgaaaagcc gaagccgaaa cctaagccaa aaccgaagcc
    16441 ggtgaaaaag gtggagcagc caaaacgcga tgtcaaaccc gtagagtcgc gtccggcatc
    16501 accgtttgaa aatacggcac tcgcgcgccc gacatcaagt acggcaacgg ctgcaaccag
    16561 caagccggtt accagtgtgg cttcaggacc acgcgcatta agccgtaatc agccgcagta
    16621 tccggcacga gcacaggcat tgcgcattga agggcaggtt aaagttaaat ttgatgtcac
    16681 gccagatggt cgcgtggata acgtacaaat cctctcagcc aagcctgcaa acatgtttga
    16741 gcgtgaggtg aaaaatgcga tgcgcagatg gcgttatgag ccgggtaagc caggcagtgg
    16801 gattgtggtg aatatcctgt ttaaaattaa cggcactacc gaaattcagt aagcataaag
    16861 tcaaaagcct ccgaccggag gcttttgatt attactcaac gggtaaggcg cgaggttttc
    16921 cttcgggatc aaccgcgaca tacttaaata atgcttctgt tgctttatag cgttgcccga
    16981 ttggttcaga cgccactttt ttcacccaca cttcaatatt aatgctgact gatgtcgtcc
    17041 ctttctggac acagcgtgca tagcagcaca ccacatcgcc gaccgcaacc ggccgtaaga
    17101 aagtcattcc ttcaacccgc acggtcacta cacgaccatg ggcaatttct ttcgccagaa
    17161 tagcgccgcc aatatccatt tgtgacatta accaaccacc aaagatgtca ccattggcat
    17221 tggtatcggc gggcatggct aaagtacgta aaacaagatc gccctgaggg acgttatgtg
    17281 ttgtagacat ggtaaaaccg acttaaaagg aaaatcacag gcgcgatgct actatgattt
    17341 tcctgaggag aacagaggag atacagcgcc agcccctgaa ggactggcag tctggttagg
    17401 atttatcttc ctgcggcatg tggcggtaga tatagatacc gcttaacaat gtgaagatta
    17461 aggtaagggc ggtcaggcca aagactttaa agttgaccca aatattttgc ggcagccaga
    17521 atgcgatgta gatgtttgcc agaccgcaaa ggataaagaa aacagcccag gccagattca
    17581 gcttcgacca taccgattgc ggcagggtga gttctttacc cagcatccgc tgaattagcg
    17641 gctttttcat cacccattgg ctgactaaca gggcccccgc aaacagggca taaatgacag
    17701 taaccttcca tttaataaac tcatcattgt ggaagaacag cgttaagcca ccgaagacga
    17761 ccaccagaac aaaagtgatc agggccatct tctcaacctt acgaaagcga acccagctat
    17821 atataagcac aatcgccgtg gcgacgatga gcgccgcagt agccgcatag atgtcataaa
    17881 tcttgtaaaa cgcgaaaaag acaaccagcg gtaaaaaatc aagaaactgc ttcattttac
    17941 gattccgtaa tcaaatgctt aaggttattg gcgaatcaac atatacaggc ggaacagata
    18001 gatgagcaat acggctgaaa tcaggttgct caaggtgttc gccagtacgg caccaatttc
    18061 cggggttaat gcggcaaaag aagaggcaaa aagcagcaac agtgtttttg ccagcaacca
    18121 gctcagtact gcgggtgcca ccagacgcat attcgcccat gtcagccgca tactgctacg
    18181 catcgaggca aaaacgccca ttttgtcctg taccagcatc actggagcca gagccagtaa
    18241 aatggccatg ataatccccg gaacgaccac cagcatgatg ccaatctgta ctaaaagggt
    18301 agtcagaaaa atcagaataa ataactttgg caatatcggc gcactggcac caatagctcg
    18361 cagcgcactg actctctgac ccgcagacac caactggata attaatatta cgcccccggc
    18421 gagaatggcg ttaccgatta atcctgaaaa agtggacgcc gctgaagcct gcagcaaaat
    18481 ttgttgctgt tccggtgaca tattctgaac caggtcgaac aacccactac tgccgctaac
    18541 gggtacgccg tcattgagct gcgcaagctg tgcatcactg ggtgagaaaa catgccctaa
    18601 caccactgtg ataaacgcac atagcaacga taccaacaga atggtcataa attgattacg
    18661 gaaaaaattt ccggtgtcac ggtatacgga ctgcgccgtg atagacatgc actctccttg
    18721 agttttatgc aggtgttaat tagcgggcaa ttgtaccctg gttaagcccc gggtggcagc
    18781 atcaggtctt gtatggaaaa gcatatcttt gtaaagcgga ggtaattgct ggccttcagg
    18841 cgtgggtaaa ccgctgcaag tggcgagcga gttaatattt gcgtagcgaa aatatttaaa
    18901 aattgattta aatcacatta accaagattt tcaatgcaac ttctaaatta atccagatca
    18961 ataaagggtg aattaccata tgtaatgtga tctgtgtagg atcatttgtt accccaatgt
    19021 gggtatattc gtcacgtttt tataaccata acgacggagc ggatatgaaa aagttaacag
    19081 tggcggcttt ggcagtagca actcttctct ctggcagtgc ctttgcgcat gaagccggcg
    19141 aattttttat gcgtgcaggt tctgcaaccg tacgtccaac agaaggtgct ggtggtacgt
    19201 taggaagtct gggtggattc agcgtgacca ataacactca actgggcctg acgtttactt
    19261 atatggcgac cgacaacatt ggtgtggaat tactggcggc atcgccgttt cgtcataaag
    19321 ttggcacagc cactaccggg gatattgcga ctgtccacca tttaccgcca acactgatgg
    19381 cacaatggta ttttggcgat tcaggcagta aattccgtcc ttacattggt gcgggtatta
    19441 actacaccac cttttttaat aatgacttca atgacaccgg tgaaggtctt ggtttatccg
    19501 accttagtct gaaggattca tggggcgtgg cggggcaagt aggtctggat tatctcatca
    19561 accgtgactg gttgctgaat atgtcggtct ggtacatgga tatcgatacc gaagtgaaat
    19621 ataaatccaa cggcgcagtg ctgcctgcag ggcgttattc tgacaacgta cgccttgacc
    19681 cgtgggtatt tatgttctct gcgggttacc gcttctaatt tttaaccatc aataaaccgc
    19741 cagatgtttt ctggcggttt gtctcaaatt aacgtcgtgt cgccgctttc atcggttgta
    19801 caaaagcttt cagtgccgcc agcattttct ctggctcatt aatatgttgc tcgatgattt
    19861 taacgatggc cgaaccagaa atcgcgcccg cagctcctgc atcaatcgct gcttttacct
    19921 gatccggggc ggaaatacca aatccctgca atggaggcgc agcattgtat tctttcagct
    19981 tcgtgaccag atgattgagg ggtaacgcgg cgcggttttc tgcgccagtc acgcccgcac
    20041 gcgacagcaa ataggtgtat ccacgaccgt aggaggctat ctggcgcagc aaatcatcgt
    20101 cggcattcgg cgggcaaata aagataggtg cgacattatg acgcaacgcg gcctggcgga
    20161 agggcgcgga ctcttccacg ggcacatcgg caaccagcac cgaatcgacg ccgactttct
    20221 cgcactcggc ataaaactca tcaatgcctt tgttaaacac caggttggca tacatcaaaa
    20281 ggccgatggg aatggtcggg tgcttctggc gaatgagtgc cagcatctca aagcactgcg
    20341 ccggggttac tcccgccgca aaagcacgca gtgtggcgtt ttgaatcgtc gggccatccg
    20401 ccagtgggtc ggagaagggg atgcctaatt ccagcgcgtc agcaccggct tcaattagcg
    20461 tatcgataat tttcaacgac tgctcaatgc ccggatcacc gagggtgacg aaaggaacga
    20521 atgcgccttc tttgcgctcc ttcaactggg caaacagaga ttcgtagcgt tccatcagat
    20581 ttcccctcgt gctttcaaaa tatcgtgaac ggtgaagatg tctttatcgc cgcgaccgga
    20641 aaggttaacc accagtagct gctctttttc cggattttcg cgcatcattt tcagcgcatg
    20701 ggccagggcg tgggaggatt ccagcgccgg gatgatccct tcatgcaggc aaagcgtttt
    20761 aaaggcttcc agggcttcat cgtcggtaat agacacgtaa tcagcgcgtc cagtgctgtt
    20821 gagatacgca tgttgcgggc cgacggacgg gaaatccagc ccggcagaaa tggagtaaga
    20881 ctcttcaatt tgcccgtctt cggtttgcat catcggcgct ttcataccga aatagatgcc
    20941 cacgcgacca tgttttaacg gtgcgccgtg ctcgccagtt tcgataccgt ggccgccagg
    21001 ctccacacca atcaggccga cgtcggtttc gttgatgaaa tctgcaaaca taccgatggc
    21061 attcgaacca ccgccaacac aggcgataac ggcatccggc aggcgacctt ctctttccag
    21121 aatctgcgct ttcgtttctt cgccaatcat ccgctgaaac tcacgcacaa tggtcgggta
    21181 aggatgcggg ccagctgcgg tacccagcat atagtgcgcg gtttcataac tgccggacca
    21241 gtcgcgtagc gcctcattac aggcatcttt cagggtcgcg gaaccgctat gtaccgggat
    21301 cacttccgca cccattaagc gcatccggaa aacgttaggc gactggcgtt caacgtcttt
    21361 ggcacccata taaatacggc atttcaggcc gagcagggcg ctggcaaggg ccgacgccac
    21421 gccatgctga ccggcaccgg tttcggcaat aatttcagtt ttacccatcc gcttcgccag
    21481 taaagcctga ccgagtacct ggttagtttt atgcgcgccg ccgtgcagca aatcttcgcg
    21541 cttcagatac agcgtggtgt tcgtcccggc tgtaatgttc tggcatttgg tcagcgcggt
    21601 tggacgcccg gcatagtttt tcagcaggtc gttgaactga gcctgaaatt caggatcttt
    21661 ttgcgcgctg acaaaagctt cttccagctg gcgcagagca ggcatcagga tttgtggcac
    21721 gtacatgccg ccaaactcac caaaataggg gttaagtaat gttgtcattg ttcctttcct
    21781 taatatgcgc gcagcgtctg gaaaaccgag gccaaaagac gtgcgtcttt gatacccggt
    21841 tgcgactcta cagcagaatt aaaatcaagc ccggcgcagc cggtttgtgc cgcttccacg
    21901 cagttatctg cgcctaagcc ccccgccagc agaacgttgc caagcgattg accgtttaac
    21961 agtgaccagt cgaaacgttg cccgcttcca ccctggccgt tgtctaaaac atatttatca
    22021 acgtgctgaa gctcgcgggc gggcagggtt tcaccgacgc ttaatgcttt ccagatggca
    22081 acgttttcag gcagcgcatc acgcagcgta tcgatataca gctgatcttc attaccatgc
    22141 agttgcactg ccgccagcga taacacctta gctttgtcca ccacatcggc aatatcgtga
    22201 ttgcggaaca cgccaacata ttgcaacggt gccgcagcca tcacttcctg cgcctgttca
    22261 acgttgacgc aacgcggtga tgtcgcaaca aaaatcaacc caccgtaaat cgcgcccgcg
    22321 tcataagctg ctttagcatc ttgcccacgc gtcaggccac acactttatt ctcacccagc
    22381 aacacccggc gcacggcggc gttcaaatcg tcatgggcca tcaacgccga accaatcaga
    22441 aaaccgttag cgaagtggct taactcgcgc acctgagcgt aagtattgat gccggattcg
    22501 ctgattaccg tcacgttgtg ccccagtttc ggtgcaagct cgcgggtacg gttgagatca
    22561 atcgacaaat cacgcagatc gcggttgttg atgccaacga cctttgcccc caatgcaatg
    22621 gcacgctcca gttcctcttc attactgact tcggtcagca cacccatctc cagactgtgg
    22681 gcgacggcgg caagctggcg atattgttcg tcatccagta ctgaaagcat taataagcag
    22741 gcatcggcct ggtaatagcg cgccagatag atctggtaag gatcgataat gaagtcttta
    22801 cataaaatcg gctgcggggc gatttggctg acgatggaga ggaaatcaaa gctcccctga
    22861 aaatatttct catcagtcag cactgaaatt gccgaagcgt aatgtttata aatggcagca
    22921 atgcgtgccg gatcgaaatc atcacggatc acgccttttg acggcgacgc ttttttgcac
    22981 tccagaataa acgccgtgcg tgcaccctgt agcgcatcat aaaaatgtcg cgtgctcggc
    23041 tgaatctcat tctgaaaact ggccagcggt tgctgctgtt tgcgggcttc tacccaaatc
    23101 gccttgtctg cgacgatttt cgctaaaacg gtttgcatta tttaccctcg tgccgccagt
    23161 gcggtgactc tgtcgtaagc ggaaccactg cgcagtacct caagaacggt ttgcgcattg
    23221 gcttgcagat cttcatggcc atgcaggcgc attaacatgg cgacattcgc cgcgacggct
    23281 gcttcgtgag cggcgtcgcc tttaccttgt aacaagcgtg ttaaaatgtc acggttttct
    23341 tccggtgttc cgcctgccag ttgctcctgg tggtagggtg tcaggccaaa atcttcagcg
    23401 gtcaactgat agctcttaat ttcgccgtca tgtagttcgg caacgattgt cggcgcgtgt
    23461 aatgaaactt catccatccc gccgctgtgc accactgccg cgcgttgata ccccagcacg
    23521 cgcaaggttt cggcaatcgg cagcaccagc tccgggctgt agacaccaat taacgccagc
    23581 ggcggatgcg ccgggttaat taatggcccc agcacattga acagtgtgcg ggttttcagt
    23641 tgctggcgaa ccggcattgc atggcggaat ccggtgtgat atttcggtgc aaagaggaaa
    23701 cagacgccta actcatccag cgcctggcgc gatttatcgg cgttcatatc aagattaata
    23761 ccgaacgccg ccagcagatc cgacgagccg gatttactgg agacgctacg gttgccgtgt
    23821 ttcgccactt tcagcccgca ggccgcggcg acaaacgcgc tggcggtaga aatattgatg
    23881 ctgttgctgc cgtcaccgcc agtaccgacg atatcggcaa acagataatc cgggcgcggg
    23941 aacggcgcgg cgttttccag tagcgcggtt gccgccccgg cgatctcgtt cgggtgctca
    24001 ccgcgaattt tcatgctcac cagcgccgcc gccagttgtt ctggtttcag ctcgccacgt
    24061 accaccgctg aaaacagctg gtggctttcc tgctggctaa gggtctgcgc ctgatacagt
    24121 ttttccagaa tcggttgcag cgtgttggtt ggctctagtt tctgctgcgc ccaggccagc
    24181 gtttgttcca gcaggcgagc gccctgggta gtaagaatgg attccggatg gaactggaat
    24241 ccacaaacgc gatccacatc atgacgcacc gccatcacca tgccattaaa atgggcgttg
    24301 atggttaaac cggccggaat attactgcca accagcgagt gataacgcgc cactggcagc
    24361 gggtttgtta atccggcaaa catcgcctga ccgtcatgtt caatgctcga cgctttaccg
    24421 tgaagaattt cgcccgcctg accgacatag cccccgtaag cttcgacaat cgcctgatgt
    24481 ccgaggcaaa tgccaataat tggcagcttg ccacgcaagc gggtgaggag ttccggcata
    24541 caaccggctt cgctcggcac accggggcca ggagaaagca tcagcaccgg attgctcatc
    24601 gtcgccaggc gttcaattaa ggtctgcgcc ggaatatggt tgcggtaaat caccacgtta
    24661 tgaccattgc tgcgcaactg atctgccagg ttgtacgtaa aagagtcgat attatcgagc
    24721 agcagaatgt cagccattag aacgtctcct gtgcatgatg cgcggtggca atagcgcgca
    24781 gtacagcgcg ggctttatta cgagtttcgt cggcttccga ctgcggaaca gaatcaagga
    24841 ctacgccagc accggcttgc acggtggcga taccgttttc caccagcgcc gagcggatca
    24901 caatgcaggt gtcgagatcg ccatgcgcgg taaaataacc taccgcgccg ccgtagctgc
    24961 cgcgtcgacg accttctgct tcggcaatta actgcatagc gcgtactttc ggtgcaccgc
    25021 ttaacgtccc catattcata caggcgcggt aagcgtgcag ggcgtcgaga tcgtggcgca
    25081 gctcaccaac aacgcgggag accaggtgca tcacgtaaga gtaacggtca actttggtga
    25141 gatcggcgac gtagcggctg ccgggtgtgc aaatgcgagc caggtcatta cgggcgagat
    25201 ccaccagcat cagatgttca gaaagctctt tatgatcggt gcgcatttcc agttcgatgc
    25261 ggctgtcgag gtctctatcc agtgaaccat cggcacgacg accgcgtgga cgtgttccgg
    25321 caatcgggta aatctcaatc tggcggctgg tggcgtcata cttgagcgaa ctttccggcg
    25381 atgcgccaaa cagggtgaaa tcattatcct gcataaaaaa catgtacggg ctgggattac
    25441 tctttttcag cacgtaatag gccgccagcg gtgacgggca gggcagagag aaacggcgag
    25501 atggcaccac ctggaaaatt tctccggcgc gaatcgcttt ttgcaacaaa cgcactacgc
    25561 caccgaactc ttcatcgctc tggttacatt cacaacgcat atgcggcacg gaaaccaccg
    25621 gcagaggcgg cgcggcttcg gtcagttgct gacgtagttc gttaaggcga gcagtgagac
    25681 gttgtttttc ttcttcattc ggagcaaaca ggctggcctg aatacgggtg ctttttttct
    25741 gatggtcaat caccatcagc gtttcagcga gataaaaaca gaaatcaggg cagttatttt
    25801 ccgctgacag ttgcggtaaa tcttcaaatc ccgccacaag gtcataagag aacaggccgc
    25861 cgaagaacat tgcttctcgt tcttccttcg gtacattcaa cagattctgt aataagcgga
    25921 aagcgtcaaa aaccgaaagg gagcataagc gggcgtcttc atccagcagt ggactgacag
    25981 gcgggaagca cagtacgcgg cagtttggtg attgttcatt ttccacaccc gcaggcaagg
    26041 cgttatccag tagtgtcaac agggcttctc cattgccgga aagtgcctgg attgtgacag
    26101 tgtcacttaa tgctgtaatg cgcagcgcac tgtctaccag cagcaggctt tttaaatcat
    26161 ctttgctgtc gatatctgcg gattccagca gcagcgttgc cgggcgatcc ccacacaact
    26221 ggtgaaaaag cgcagtcggg ttgtcgcgat aagcgccttt gcaggttagc agttcgagag
    26281 tcggtttttg tgtttgcatt gttattctct aattttgttc aaaaaaaagc ccgctcatta
    26341 ggcgggctgg gtatctgatt gctttacgca tggtgaatac actgcccgtt tcaggaagtg
    26401 cgccaccaac ctttcagtac gaaaattgct ttcattgtcg ataccctttt tacgtgaact
    26461 tgcgtactag ttaactagtt cgatgattaa ttgtcaacag ctcatttcag aatatttgcc
    26521 agaaccgtta tgatgtcggc gcaaaaaaca ttatccagaa cgggagtgcg ccttgagcga
    26581 cacgaattac gcagtgattt acgacctgca cagccatacc acagcttccg atggctgcct
    26641 gacgccagaa gcattggtgc accgtgcagt cgagatgcgc gtcggcaccc tggcgatcac
    26701 cgaccatgac accacagctg caattgcacc agcaagagag gaaatttcac gttcgggact
    26761 ggcgctgaat cttattcctg gtgtggaaat ttccacggtc tgggaaaatc atgaaattca
    26821 tattgtcggg ctgaatattg atattactca tccgctaatg tgcgagttcc tcgcgcagca
    26881 gacagaacgg cgcaatcagc gggcgcagct gattgccgaa agacttgaaa aagcgcaaat
    26941 acctggggca ctggaaggcg cgcaacgact ggcgcagggc ggtgcggtga cgcgcggtca
    27001 ttttgcgcgt ttccttgtgg agtgcggaaa agccagttca atggcggatg tctttaaaaa
    27061 gtatctggcg cgcgggaaaa ccggatacgt tccgccacag tggtgtacaa tagagcaatc
    27121 tattgatgtc attcatcatt ctggcggtaa ggcggtgcta gctcatcctg ggcggtacaa
    27181 tcttagtgct aaatggctga aaagattggt tgcgcatttt gccgaacacc acggcgacgc
    27241 gatggaagtc gcgcagtgcc agcaatcgcc caatgaacgc acccagctgg cgacacttgc
    27301 gcgtcagcat catttatggg catcacaagg atctgatttt catcagccat gcccgtggat
    27361 cgaactgggt cgtaaactct ggctgcccgc gggcgttgaa ggcgtctggc agctatggga
    27421 acagccgcag aacaccacag agagggaatt atgagccagt ttttttatat tcatcctgat
    27481 aacccgcagc aacgcctgat caaccaggcg gtggagatcg tgcgcaaagg cggggtgatt
    27541 gtttatccaa ctgattccgg ctatgcgctc ggctgtaaaa ttgaagataa aaacgcgatg
    27601 gagcgtattt gtcgtattcg ccagctgccg gacggtcaca actttaccct gatgtgtcgc
    27661 gatctttctg aactctcgac ctattcattt gtcgataacg ttgcgtttcg tttgatgaaa
    27721 aacaacacgc caggcaacta taccttcatc ctgaagggga cgaaggaggt gccacgccgt
    27781 ctgttgcagg aaaaacgcaa aaccatcggt atgcgcgtgc cgtctaaccc gattgcgcag
    27841 gcgctgcttg aagcattggg cgaaccgatg ctttccactt cgctaatgct gccaggcagt
    27901 gaatttaccg aatccgatcc ggaagaaatt aaagaccgtc tggaaaaaca ggtggatttg
    27961 attattcacg gcggctatct cgggcagaaa ccaacaacgg ttattgatct caccgatgat
    28021 acgccggtcg tggtgcgtga aggcgtaggt gatgtgaagc ctttcttata acaaattgcg
    28081 ttccacggat ggaagatgat ggcagggata tatcgctgca ttttgctgtt gatagttggg
    28141 ctttttttct cttcgttgag ttatgcgaaa aacacggaga tcccttctta tgaagaaggg
    28201 atctcgctct ttgatgttga agccactctg caaccgaatg gggtgctcga catcaaagag
    28261 aacattcatt ttcaggcgcg aaatcagcag attaagcacg gattttatcg tgatttaccg
    28321 cgactctgga tgcagcctga tggggacgct gcactgctga actatcatat tgttggcgtc
    28381 acccgtgatg gtattcctga accctggcat cttgactggc atatcgggtt aatgagtatt
    28441 gtcgtgggcg ataaacaacg tttcttgcct caaggcgact atcattatca aattcattat
    28501 caggttaaaa atgctttcct gcgtgagggg gattctgatc tgctaatctg gaacgtgacc
    28561 ggtaaccact ggccgtttga aatttataag accctatttt cactcaagtt gcctgatatt
    28621 gcgggtaatc catttagcga aatcgatctc tttactggag aagagggcga cacatatcga
    28681 aatggccgca tccttgagga cggaagaatt gaatctcgcg atccgtttta tcgtgaagat
    28741 ttcacggtcc tctaccgctg gcctcacgca ttacttggta atgccccggc tccacaaacg
    28801 acgaatattt tcagtcatct tcttttaccc tctacgtcat cgttgttaat ttgctttccg
    28861 agtctgttcc tggcgtgtgg atggttatat ctctggaagc gcaggccgca atttacgccg
    28921 gtagatgtga ttgaaaccga tgtcattccg ccagattaca cgcccggcat gttacgtctt
    28981 gatgcgaaac tggtttacga cgataaaggt ttttgcgccg atatcgtaaa tctgattgtg
    29041 aaaggaaaaa ttcatctgga agatcattat gacaagaacc agcaaatcct gattcgtgtt
    29101 aatgaaggcg cgaccagaaa taatgcggta ttactgcccg cagagcagtt attactggaa
    29161 gcgttatttc gtaaaggcga taaggtcgtg cttacgggga gacgcaacag agtcttacgc
    29221 aaggcatttt tacggatgca gaaattttat ctgccgcgta aaaagtcttc gttttaccga
    29281 cctgatgcgt ttttgcaatg gggcggaatg gcaatattgg cggtcattct ctacggtaac
    29341 ctgagtcccg taggctgggc aggaatgagt ctggtaggcg atatgtttat tatgatctgc
    29401 tggcttcttc cttttttatt ttgttccctt gagcttttgt ttgcccgcga tgatgacaag
    29461 ccttgcgtta atcgtgtcat catcactttg tttttaccac tgatttgttc aggtgtggct
    29521 ttttactctc tctacatcaa tgtcggagat gtattctttt actggtatat gccagcgggt
    29581 tattttagcg ctgttttcct gaccggttat ctcactggca tggggtatat ttttctgcca
    29641 aagtttaccc aaactgggca gcaacgttat gccaacggtg aagctatcgt taacgatctt
    29701 gcgcgtaaag aggcagcaac acacagtggg cggcggagga aaggggaaac acggaaactg
    29761 gattacgcgt tgttaggttg ggctatatcg gcaaatttgg ggagggaatg ggcgttacgc
    29821 atcgcccctt cgctttcttc ggcgatttgc gctccagaga ttgcccgtaa cggcgtttta
    29881 ttctcattac agacgcacct gagttgcggg gcctatacca gtttgttggg gcggagctat
    29941 tctggtggtg gttctggcgc tggtggcgga ggcggtggtg gctggtaatc aagctgatgt
    30001 taatcgaaac aagctttaac cataaagata gtgtagtatg ttgcgcctca aagccaggca
    30061 agttaaaacg tcgagtcgtt tacttaaggc ctgaagagtt caaacagact ttactatcag
    30121 tgagtcagat gtaagttctg actcattgtt gcacaaaagc atatcagacg cctgggaagg
    30181 cgacaccaaa aggaagctct atgagcgaaa agctacagaa agtgctggcg cgtgccggcc
    30241 acggttctcg ccgtgaaatc gaatctatta ttgaagccgg tcgtgtgagt gttgatggca
    30301 aaattgctaa actcggcgat cgcgttgaaa ttacccctgg actgaaaatc cgtatcgatg
    30361 gtcacctgat ttcggtacgt gagtccgctg aacaaatttg tcgcgttctg gcctattaca
    30421 aaccggaagg tgagttgtgt acccgtaacg atccggaagg gcgtccaacg gtgtttgacc
    30481 gtctgccaaa gctgcgtggc gcacgctgga ttgccgtagg tcgtctggac gttaacacct
    30541 gtggtctgtt gttgttcacc accgacggtg aactggcaaa ccgtttaatg catccaagcc
    30601 gtgaagttga acgtgaatat gccgtgcgtg tatttggtca ggttgacgac gcgaaactgc
    30661 gtgatttgag tcgtggcgtg cagctggaag atggtccggc agcctttaaa accatcaagt
    30721 tcagcggcgg cgaagggatc aaccagtggt acaacgttac tctgaccgaa ggccgtaacc
    30781 gtgaagttcg tcgtctttgg gaagcggttg gtgtgcaggt gagccgcctg attcgtgttc
    30841 gttacggtga tatcccactg ccaaaaggtc tgccacgcgg tggctggacg gagctggacc
    30901 tcgcccagac taactatctg cgcgaactgg tggaactacc gccagaaacc agctctaaag
    30961 tcgctgtaga aaaagaccgt cgtcgtatga aggcgaatca gattcgtcgt gcggtgaaac
    31021 gtcacagtca ggtgagcggc ggtcgtcgtt ctggcggacg taataataac ggttaatcaa
    31081 agtatgccgg acgtcatatc cggcattttt acagattagt aatcaatccc tatctgcgct
    31141 tttacaccgg catcaaacgc atgtttgacg gggcgtaatt cacttaccgt atccgccagt
    31201 tcaagaatat cccgatgaca accacgacct gtgataatca ccgtctgttg atgtggacgt
    31261 tcatttaacg cctgcatcac ttcttccagt ggcaaatagt catacgccac catatacgtc
    31321 agttcatcga gcaaaaccat atccagtgag gaatcagcca gcatccgctt tgcatgttgc
    31381 cagacttcgc ggcaggcggc ggtatcagac tctcggtttt gtgtatccca ggtaaagccc
    31441 gttgccatta cctgaaactc aacgccgtgt ggctccagca gatttcgttc accattaggc
    31501 caggtgcctt taataaactg cacgacgccc actttttttc cgtgaccaac tgcgcgtgtt
    31561 gccgtaccaa atgccgcggt ggttttgcct tttccattgc cggtaaagac gatgataata
    31621 ccgcgttcat cctgggcctg ggccacacga gcatctactt tttctttcac tcgctgctga
    31681 cgctgttggt agcgttcatc actcattggg aaattcctgg tttacggccc ggttgggcgt
    31741 caaaggtcat gccggtttta cggcggctgt catcgcccat cagccagagg tagagcggca
    31801 tgatatcagc gggggtttta agtttctgtg gatcttcggt cgggaaggcg ctggcccgca
    31861 ttgcggtgcg cgtaccgcct gggttaatgc agttgacgcg caggtgctgc tgatattcat
    31921 cggccagtac ctgcatcatc ccttcggtgg caaatttcga cgctgcatat gcgccccagt
    31981 ttgcacgccc ctgacgtcca acgcttgatg aggtaaagac cagtgaaccg gcatccgatt
    32041 tgagtaataa aggaagcagt gcctgtgtga gcataaaggt ggcattaacg ttgacctgca
    32101 tgacgtcctg ccagacctgc ggatcttgtt cgctcattgg gcaaacatcg ccgagcaatc
    32161 cggcattatg caaaacacca tccagacgcg gataattaac ggcaatgcgc tgtgccagtt
    32221 gttggcaatc ttcggacgtg caggttagca aatcgagaat aaaccactgt ggctgacgtc
    32281 cagtttcttc gtttatgtgg ctggctacct gacgtaattt ttcttcatta cggcccaaaa
    32341 gaatcactgt cgcaccatag cgtgcatacg tcatcgctgc ttcacgacca attccatcgc
    32401 tggctcccgt caccaggata atgcggtcat tgagtaaatc ttgttttggc tggtaatgca
    32461 tggctactcc tcaacgacgt tgtctgtcgc gctgccagtg tactttatga ctttggggct
    32521 ttatgcctga aacagaaggg catttcaatc agcctggggc gataacaccg caaaatggat
    32581 actttgtcat actttcgctg caataacatc tctgcgagac ggctaaacat gcctgttgta
    32641 aactgtgagc caaagcgttg tttaaccaag gtggggactc gtggaattgt tgtctgaata
    32701 tggtttgttt ttggcgaaaa tcgttaccgt tgtgctagcg attgcggcga ttgccgccat
    32761 tattgtcaat gttgctcaac gtaataaacg ccagcgtggc gagttacgag tcaacaatct
    32821 cagcgaacag tataaggaga tgaaagaaga actggccgcg gcgctgatgg acactcatca
    32881 gcaaaaacag tggcacaaag cgcagaagaa aaagcacaag caagaagcga aagccgcaaa
    32941 agcgaaagct aaactgggcg aggtggtaac tgacagtaaa ccccgcgtct gggtgctgga
    33001 ttttaaaggc agcatggacg cccatgaagt gaactcgcta cgtgaagaga taacggcggt
    33061 actcgcagca ttcaaaccgc aggatcaggt tgtgctccgt ctggaaagcc caggtggcat
    33121 ggtgcatggt tacgggttgg cggcttcgca gttgcagcgt ctgcgtgata aaaacattcc
    33181 tttaactgtt acggtagaca aagtcgctgc cagcggcggt tacatgatgg cctgtgtggc
    33241 ggacaaaatt gtttccgcac cgtttgctat tgtgggttcc attggggtgg tggcgcaaat
    33301 gcccaacttt aaccgcttcc tgaaaagcaa agatattgat atcgaactgc acaccgccgg
    33361 gcagtataag cgcacgctga cgttgctggg tgaaaatacc gaagaagggc gggagaaatt
    33421 ccgcgaagag ttgaacgaaa cgcatcagtt atttaaagat tttgtgaagc gtatgcgtcc
    33481 gtctctggat attgaacagg tggcaacggg tgaacactgg tacggacaac aggcagtaga
    33541 gaaaggcctg gttgatgaaa tcaacaccag tgatgaagtt attcttagcc tgatggaagg
    33601 ccgggaagtg gtcaatgtac gctatatgca gcgtaaacga ctcattgacc gattcaccgg
    33661 cagcgcggca gagagcgccg atcgattgtt gttacgctgg tggcagcgtg ggcaaaagcc
    33721 gttgatgtaa aagacaaacg cgaggataag acctcgcgtt ttgctttaat caaccaggtg
    33781 atatttttct gaaagcacat gggccaggtg tttgaacata ttaaacaccg cggtgctttt
    33841 ggcagttggt aatccttgtt catctaaaaa gtagtcgccg gtaaatacca gcacgccatt
    33901 acgctgcgtg acgccggtgg cttcaatccc tgcgagcgta tcctcatgct cacgaatgat
    33961 tttgttggcc tctttcaaca gcgtttcgcg gtcgatgggt tgagtctctt tgttcattat
    34021 ttactcctta aacaaggaca ttagtctacg ccaggcatgg cttgcagaca aatataccac
    34081 gctggtggca agagcgcctt actggcaact ttggattttg catgctaata aagttgcgta
    34141 tcggatttta tcaggtacag tgtgacgctt tcgtcaatct ggcaatagat ttgcttgaca
    34201 ttcgaccaaa attccgtcgt gctatagcgc ctgtaggcca agacctgtta actcagtcac
    34261 ctgaattttc gtgagcagag tcacgacaag gggttgatat ccgcagagag cgagtccata
    34321 tcggtaactc gttgccagtg gaaggtttat caacgtgcga cgcattcctg gaagaatcaa
    34381 attaggtaag gtgaatatgg gtaaagctct tgtcatcgtt gagtccccgg caaaagccaa
    34441 aacgatcaac aagtatctgg gtagtgacta cgtggtgaaa tccagcgtcg gtcacatccg
    34501 cgatttgccg accagtggct cagctgccaa aaagagtgcc gactctacct ccaccaagac
    34561 ggctaaaaag cctaaaaagg atgaacgtgg cgctctcgtc aaccgtatgg gggttgaccc
    34621 gtggcacaat tgggaggcgc actatgaagt gttgcctggt aaagagaagg tcgtctctga
    34681 actgaaacaa ctggctgaaa aagccgacca catctatctc gcaaccgacc ttgaccgcga
    34741 aggggaagcc attgcatggc acctgcggga agtgattggt ggtgatgatg cgcgctatag
    34801 ccgagtggtg tttaacgaaa ttactaaaaa cgcgatccgc caggcattta acaaaccggg
    34861 tgagctgaat attgatcgtg ttaatgccca gcaggcgcgt cgctttatgg accgcgtggt
    34921 ggggtatatg gtttcgccgc ttctatggaa aaagatcgct cgtggtctgt ctgccggtcg
    34981 tgtgcagtcg gtggcggtcc gcctggtggt cgagcgtgag cgtgaaatta aagcgttcgt
    35041 accggaagag ttctgggaag tcgatgccag cacgaccacg ccatctggtg aagcgttggc
    35101 gttgcaggtg actcatcaga acgacaaacc gttccgtccg gtcaacaaag aacaaactca
    35161 ggctgcggta agtctgctgg aaaaagcgcg ctacagcgtg ctggaacgtg aagacaaacc
    35221 gacaaccagt aaacctggcg ctccttttat tacctctacg ctgcaacaag ctgccagcac
    35281 ccgtcttggt tttggcgtga aaaaaaccat gatgatggcg cagcgtttgt atgaagcagg
    35341 ctatatcact tacatgcgta ccgactccac caacctgagt caggacgcgg taaatatggt
    35401 tcgcggttat atcagcgata attttggtaa gaaatatctg ccggaaagtc cgaatcagta
    35461 cgccagcaaa gaaaactcac aggaagcgca cgaagcgatt cgtccttctg acgtcaatgt
    35521 gatggcggaa tcgttgaagg atatggaagc agatgcgcag aaactgtacc agttaatctg
    35581 gcgtcagttc gtagcctgcc agatgacccc agcgaaatat gactccacga cgctgaccgt
    35641 tggtgcgggc gatttccgcc tgaaagcacg cggtcgtatt ttgcgctttg atggctggac
    35701 gaacgtgatg cctgcactgc gtaaaggcga tgaagatcgt atcttaccgg cagtcgataa
    35761 aggcgatgct ctgacgctcg ttgaactgac accagcccag cactttacca agcctccagc
    35821 ccgtttcagt gaagcatcgc tggtcaaaga gctggaaaaa cgcggtatcg gtcgtccttc
    35881 tacctatgcg tcgatcattt cgaccattca ggatcgtggc tatgtgcgag tagaaaatcg
    35941 tcgtttctat gcggaaaaaa tgggcgaaat cgtcaccgat cgcctggaag agaatttccg
    36001 cgagttaatg aactacgact tcaccgcgca gatggaaaac agcctcgacc aggtggcaaa
    36061 tcacgaagca gagtggaaag ctgtactgga tcacttcttc tcggatttca cccagcagtt
    36121 agataaagct gaaaaagatc cggaagaggg gggtatgcgc ccgaaccaga tggttctgac
    36181 cagcattgac tgcccgactt gtggtcgcaa aatggggatt cgcacagcga gcaccggggt
    36241 attccttggc tgttctggct atgcgctgcc gccgaaagag cgttgcaaaa ccaccattaa
    36301 cctggtgccg gaaaacgaag tgctgaacgt gctggaaggt gaagacgctg aaaccaatgc
    36361 gttgcgcgca aaacgtcgtt gcccgaaatg cggcacggcg atggacagct atctcatcga
    36421 tccgaaacgt aagttgcatg tctgtggtaa taacccaacc tgcgacggtt acgagatcga
    36481 agagggtgaa ttccgcatta aaggttatga cggcccgatc gttgagtgtg aaaaatgtgg
    36541 ctctgaaatg cacctgaaaa tggggcgttt cggtaaatac atggcctgca ccaacgaaga
    36601 gtgtaaaaac acacgtaaga ttttacgtaa cggcgaagtg gcaccaccga aagaagatcc
    36661 ggtgccatta ccggagctgc cgtgcgaaaa atcagatgcc tatttcgtgt tgcgtgacgg
    36721 tgctgccggt gtgttcctgg cggccaatac cttcccgaaa tcgcgtgaaa cgcgtgcgcc
    36781 gctggtggag gagctttatc gcttccgcga ccgtctgccg gaaaaactgc gttatctggc
    36841 cgatgcgcca cagcaggatc cggaaggtaa taagaccatg gttcgcttta gccgtaaaac
    36901 caaacagcaa tatgtctctt cggaaaaaga cggaaaggcg actggctggt cagcatttta
    36961 tgttgatggc aaatgggttg aagggaaaaa ataaccttta attctgtcag gtttttataa
    37021 ac
//
LOCUS       DADSYM010000025        36619 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2415
            SAMN08039579-rid11723173.denovo.025, whole genome shotgun sequence.
ACCESSION   DADSYM010000025 DADSYM010000000
VERSION     DADSYM010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039579
            Sequence Read Archive: SRR6362533
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 36619)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36619)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980162.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 03:39:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,484
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 4,347
            CDSs (with protein)               :: 4,347
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 43 of 85
            Pseudo Genes (incomplete)         :: 37 of 85
            Pseudo Genes (internal stop)      :: 19 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36619
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039579-rid11723173.denovo.025"
                     /strain="LGE2415"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8302 N 95.9628 W"
                     /collection_date="2015-08-24"
     gene            196..786
                     /locus_tag="J1Y83_001070"
     CDS             196..786
                     /locus_tag="J1Y83_001070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HBA7629239.1"
                     /translation="MSKKLGFVLSGIMLAMAAGTAFAANDMDGGQLNISGLVVDNTCE
                     ARVDGGNKDGLILLQTATVAEITDGVLDTTVGAKAKPFSITIDCSKANPAPGTTAKMT
                     FGSVFFGNSKGTLNNDMSINTPSDGVNIALHNIEGSIIKQVQVNNPGDVYSKTLDSTS
                     KSATYDFKASYVRADANKAATAGYVKTNSAYTITYQ"
     gene            895..1635
                     /locus_tag="J1Y83_001071"
     CDS             895..1635
                     /locus_tag="J1Y83_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone"
                     /protein_id="HBA7629240.1"
                     /translation="MFFNTKHTAALCLASCMAFSSSAIADIVISGTRVVYKSEQKNVN
                     VRLENKGNNPLLVQSWLDTGDDNAEPGSITVPFTATPPVSRIDAKRGQTIKLMYTASS
                     ALPKDRESVFWFNVLEVPPKPDAAKAANQSLLQLAFRTRIKLFYRPEGLSGMPSDAPL
                     ALKWSWATSEGKAALRVDNPTPYYVSFSSGDLEANGKRYPLDMTMIAPFSNDVIKVNG
                     MSGRTSSAKVHFFAINDFGGSIEGNANL"
     gene            1670..4258
                     /locus_tag="J1Y83_001072"
                     /pseudo
     CDS             1670..4258
                     /locus_tag="J1Y83_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414681.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane usher protein"
     gene            4275..4847
                     /locus_tag="J1Y83_001073"
     CDS             4275..4847
                     /locus_tag="J1Y83_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414680.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HBA7629241.1"
                     /translation="MIKTTPHKITILMGLLLSPSVFATDVNVDFTATVKATTCNITLT
                     SLNGSNVVDNGNDSYTLIIPNMGLDKIVNKASQSEANFKLVASGCSSGISWIDTTMTG
                     NQSGSTSLIIPQSSDSSSTTSNIGMGFKRLETSGDTFLKPNSAEYIRWKTTEISTSGL
                     EMTVALREITPGQGIPGKFRAQATFNFSYQ"
     gene            4859..5467
                     /gene="yadL"
                     /locus_tag="J1Y83_001074"
     CDS             4859..5467
                     /gene="yadL"
                     /locus_tag="J1Y83_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020235143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial-like protein YadL"
                     /protein_id="HBA7629242.1"
                     /translation="MMILKKLCYGLSGSLFLASSLLTISSHAATDSIGLTVTTDVEMG
                     TCTSTLTDDGTAKLSTINFGDVYISEINAETKIKTFKLQFKDCAGIPGKKAKIKLTPR
                     ALCEGNSNNGPGFANASTAAAKAAAVAVEVWSTSTPGKNGAKQFSCVTPATEEVSIAN
                     ATGSNVVDYPMSAVLVVAKDKTVTDVTAGDFTAPATFTVTYN"
     gene            5494..6090
                     /locus_tag="J1Y83_001075"
     CDS             5494..6090
                     /locus_tag="J1Y83_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HBA7629243.1"
                     /translation="MRPTQRNLMKKSILILSLLFSATSPVVAGQDVDIVANIKNNTCQ
                     SGISNNGNIDLGVVGVGDFTGNISAENYHPGGKEFTITVQDCTLQGTGNVLNQLHIDF
                     RALSGVMATGSSQIFANEDNTGAKNVGVVIFSIQDPANTFNVLGATGSSRSVYPVMSS
                     ALNNSSWKFSTRMQKIDPTLNVTSGPLISHVLVDIYYE"
     gene            6140..7405
                     /locus_tag="J1Y83_001076"
     CDS             6140..7405
                     /locus_tag="J1Y83_001076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000848455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial-like adhesin"
                     /protein_id="HBA7629244.1"
                     /translation="MKTFFRYFLFLALCSCCYTASAGTDDNVSYLVGNDYGVGPGDQK
                     WRETGPSADITVKFRYSTSTNNLVFYKPTQLGPTGVSLYWSQLNSASGGGYLYCNRSN
                     TASGEPMAIENQMVDSGKRYGGHKLFETSVPGLYYTLKISNIWSSYTTTNISSSGMYI
                     GDSPTQYFRWIPESEATLYWSCNNANSSNKYWAVGGVWQSLTVEFYTDTNFDPTKTNK
                     VTLSKPGGYLYSFKGYGLGGGINYHSYFLKIDFDLDNMTLSNPTCFTAALTGPSVSGS
                     TVKMGDYTPAQIKNGATAVPFDITLQNCIRVRNIETKLMSNKVGSVSKELLANTLTGN
                     NAAKGVGVLIEGLENTKSAQMVLKPNDSTSIYKDYETEDDTTGGIYPDKGNGTSQPLH
                     FQATLKQDGNIAIEPGDFKATSTFQVTYP"
     gene            7517..8311
                     /gene="panB"
                     /locus_tag="J1Y83_001077"
     CDS             7517..8311
                     /gene="panB"
                     /locus_tag="J1Y83_001077"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HBA7629245.1"
                     /translation="MKPTTIASLQKCKQDKKRFATITAYDYSFAKLFAEEGLNVMLVG
                     DSLGMTVQGHDSTLPVTVADIAYHTAAVRRGAPNCLLLADLPFMAYATPEQAFENAAT
                     VMRAGANMVKIEGGEWLVETVQMLTERAVPVCGHLGLTPQSVNIFGGYKVQGRGDEAG
                     DRLLSDALALEAAGAQLLVLECVPVELAKRITEALAIPVIGIGAGNVTDGQILVMHDA
                     FGITGGHIPKFAKNFLAETGDIRAAVRQYMAEVESGVYPGEEHSFH"
     gene            8323..9174
                     /gene="panC"
                     /locus_tag="J1Y83_001078"
     CDS             8323..9174
                     /gene="panC"
                     /locus_tag="J1Y83_001078"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HBA7629246.1"
                     /translation="MLIIETLPLLRQQIRRLRMEGKRVALVPTMGNLHDGHMKLVDEA
                     KARADVVVVSIFVNPMQFDRPEDLARYPRTLQEDCEKLNKRKVDLVFAPSVKEIYPNG
                     TETHTYVDVPGLSTMLEGASRPGHFRGVSTIVSKLFNLVQPDIACFGEKDFQQLALIR
                     KMVADMGFDIEIIGVPIMRAKDGLALSSRNGYLTAEQRKIAPGLYKVLSSIADKLQAG
                     ERDLDEIIAIAGQELNEKGFRSDDIQIRDADTLLEVSENSKRAVILVAAWLGDARLID
                     NKLVELA"
     gene            complement(9256..10140)
                     /locus_tag="J1Y83_001079"
     CDS             complement(9256..10140)
                     /locus_tag="J1Y83_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HBA7629247.1"
                     /translation="MDAPSTTPHDAVFKQFLMHAETARDFLDIHLPAELRELCDLDTL
                     HLESGSFIEESLKGHSTDVLYSVQMQGSTGYLHVVIEHQSKPDKKMAFRMMRYSIAAM
                     HRHLEADHDKLPLVVPILFYQGEATPYPLSMCWFDMFYSPELARRVYNSPFPLVDITI
                     TPDDEIMQHRRIAILELLQKHIRQRDLMLLLEQLVTLIDEGYTSGSQLVAMQNYMLQR
                     GHTEQADLFYGVVRDRETGGESMMTLAQWFEEKGIQQGRQEVSQEFALRLLSKGMSRE
                     DVAEMANLPLAEIDKMIN"
     gene            10414..10794
                     /gene="panD"
                     /locus_tag="J1Y83_001080"
     CDS             10414..10794
                     /gene="panD"
                     /locus_tag="J1Y83_001080"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HBA7629248.1"
                     /translation="MIRTMLQGKLHRVKVTHADLHYEGSCAIDQDFLDAAGILENEAI
                     DIWNVTNGKRFSTYAIAAERGSRIISVNGAAAHCASVGDIVIIASFVTMPDEEARTWR
                     PNVAYFEGDNEMKRTAKAIPVQVA"
     gene            complement(10798..12027)
                     /locus_tag="J1Y83_001081"
     CDS             complement(10798..12027)
                     /locus_tag="J1Y83_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HBA7629249.1"
                     /translation="MYKQAVILLLMLFTASVSAALPARYMQTIENAAVWAQIGDKMVT
                     VGNIRAGQIIAVEPTAASYYVFNFGFGKGFIDKGHLEPVQGRQKVEDGLGDLNKPLSN
                     QNLITWKDTPVYNAPSVGSAPFGVLADNLRYPILHKLKDRLNQTWYQIRIGDRLAYIS
                     ALDAQPDNGLPVLTYHHILRDEENTRFRHTSTTTSVRAFNNQMAWLRDRGYATLSMAQ
                     LEGYVKNKINLPARAVVITFDDGLKSVSRYAYPVLKQYGMKATAFIVTSRIKRHPQKW
                     NPKSLQFMSVSELNEIRDVFDFQSHTHFLHRIDGYRRPILLSRSEHNILFDFARSRRA
                     LAQFNPHILYLSYPFGGFNDKAVKAAKEAGFHLAVTTMKGKVKPGDNPLLLKRLYILR
                     TDSLETMSRLVSNQPQG"
     gene            complement(12091..12531)
                     /locus_tag="J1Y83_001082"
     CDS             complement(12091..12531)
                     /locus_tag="J1Y83_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBA7629250.1"
                     /translation="MLGWVITCHDDRAQELLDALEKKHGALLQCRAVNFWRGLSSNML
                     SRMMCDALHETDSGEGVIFLTDIAGAPPYRVASLLSHKHSRCEVISGVTLPLIEQMMA
                     CRETMTSSAFREHIVELGAPEVSSLWHQQQKNPPFVLKHNLYEY"
     gene            complement(12636..13406)
                     /locus_tag="J1Y83_001083"
     CDS             complement(12636..13406)
                     /locus_tag="J1Y83_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001747261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBA7629251.1"
                     /translation="MMHLYWVALKSIWAKEIHRFMRIWVQTLVPPVITMTLYFIIFGN
                     LIGSRIGDMHGFSYMQFIVPGLIMMSVITNAYANVASSFFGAKFQRNIEELLVAPVPT
                     HVIIAGYVGGGVARGLFVGILVTAISLFFVPFQVHSWVFVALTLVLTAVLFSLAGLLN
                     GVFAKTFDDISLVPTFVLTPLTYLGGVFYSLTLLPPFWQGLSHLNPIVYMISGFRYGF
                     LGINDVPLVTTFGVLVVFIVAFYLICWSLIQRGRGLRS"
     gene            complement(13403..14329)
                     /locus_tag="J1Y83_001084"
     CDS             complement(13403..14329)
                     /locus_tag="J1Y83_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001621186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBA7629252.1"
                     /translation="MTIALELQQLKKTYPGGVQALRGIDLQVEAGDFYALLGPNGAGK
                     STTIGIISSLVNKTSGRVSVFGYDLEKDVVNAKRQLGLVPQEFNFNPFETVQQIVVNQ
                     AGYYGVERKEAYIRSEKYLKQLDLWGKRNERARMLSGGMKRRLMIARALMHEPKLLIL
                     DEPTAGVDIELRRSMWGFLKDLNDKGTTIILTTHYLEEAEMLCRNIGIIQHGELVENT
                     SMKALLAKLKSETFILDLAPKSPLPKLDGYQYRLVDTATLEVEVLREQGINSVFTQLS
                     EQGIQVLSMRNKANRLEELFVSLVNEKQGDRT"
     gene            14438..15100
                     /gene="can"
                     /locus_tag="J1Y83_001085"
     CDS             14438..15100
                     /gene="can"
                     /locus_tag="J1Y83_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonate dehydratase"
                     /protein_id="HBA7629253.1"
                     /translation="MKDIDTLISNNALWSKMLVEEDPGFFEKLAQAQKPRFLWIGCSD
                     SRVPAERLTGLEPGELFVHRNVANLVIHTDLNCLSVVQYAVDVLEVEHIIICGHYGCG
                     GVQAAVENPELGLINNWLLHIRDIWFKHSSLLGEMPQERRLDTLCELNVMEQVYNLGH
                     STIMQSAWKRGQKVTIHGWAYGIHDGLLRDLDVTATNRETLEQRYRHGISNLKLKHAN
                     HK"
     gene            complement(15141..15677)
                     /gene="hpt"
                     /locus_tag="J1Y83_001086"
     CDS             complement(15141..15677)
                     /gene="hpt"
                     /locus_tag="J1Y83_001086"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HBA7629254.1"
                     /translation="MKHTVEVMIPEAEIKARIAELGRQITERYKDSGSDMVLVGLLRG
                     SFMFMADLCREVQVSHEVDFMTASSYGSGMSTTRDVKILKDLDEDIRGKDVLIVEDII
                     DSGNTLSKVREILSLREPKSLAICTLLDKPSRREVNVPVEFIGFSIPDEFVVGYGIDY
                     AQRYRHLPYIGKVILLDE"
     gene            15883..18273
                     /locus_tag="J1Y83_001087"
     CDS             15883..18273
                     /locus_tag="J1Y83_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose/quinate/shikimate family membrane-bound
                     PQQ-dependent dehydrogenase"
                     /protein_id="HBA7629255.1"
                     /translation="MAINNTGSRRLLVTLTALFAALCGLYLLIGGGWLVVIGGSWYYP
                     IAGLVMLGVAWMLWRSKRAALWLYAALLLGTMIWGVWEVGFDFWALTPRSDILVFFGI
                     WLILPFVWRRLVIPASGAVAALVVALLISGGILTWAGFNDPQEISGTLSADTTPAEAI
                     SPVADQDWPAYGRNQEGQRFSPLKQINADNVHKLKEAWVFRTGDVKQPNDPGEITNEV
                     TPIKVGDTLYLCTAHQRLFALDAASGKEKWHYDPELKTNESFQHVTCRGVSYHEAKAE
                     TASPEVMADCPRRIILPVNDGRLIAINAENGKLCETFANKGVLNLQSNMPDTKPGLYE
                     PTSPPIITDKTIVMAGSVTDNFSTRETSGVIRGFDVNTGELLWAFDPGAKDPNAIPSD
                     EHTFTFNSPNSWAPAAYDAKLDLVYLPMGVTTPDIWGGNRTPEQERYASSILALNATT
                     GKLAWSYQTVHHDLWDMDLPAQPTLADITVNGQKVPVIYAPAKTGNIFVLDRRNGELV
                     VPAPEKPVPQGAAKGDYVTPTQPFSELSFRPTKDLSGADMWGATMFDQLVCRVMFHQM
                     RYEGIFTPPSEQGTLVFPGNLGMFEWGGISVDPNREVAIANPMALPFVSKLIPRGPGN
                     PMEQPKDAKGTGTESGIQPQYGVPYGVTLNPFLSPFGLPCKQPAWGYISALDLKTNEV
                     VWKKRIGTPQDSMPFPMPVPVPFNMGMPMLGGPISTAGNVLFIAATADNYLRAYNMSN
                     GEKLWQGRLPAGGQATPMTYEVNGKQYVVISAGGHGSFGTKMGDYIVAYALPDDVK"
     gene            complement(18530..20080)
                     /gene="cueO"
                     /locus_tag="J1Y83_001088"
     CDS             complement(18530..20080)
                     /gene="cueO"
                     /locus_tag="J1Y83_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase CueO"
                     /protein_id="HBA7629256.1"
                     /translation="MQRRDFLKYSVALGVASALPLWSRAVFAAERPTLPIPDLLTTDA
                     RNRIQLTIGAGQSTFGGKTATTWGYNGNLLGPAVKLQRGKAVTVDIYNQLTEETTLHW
                     HGLEVPGEVDGGPQGIIPPGGKRSVTLNVDQPAATCWFHPHQHGKTGRQVAMGLAGLV
                     VIEDDEILKLMLPKQWGIDDVPVIVQDKKFNADGQIDYQLDVMTAAVGWFGDTLLTNG
                     VIYPQHAAPRGWLRLRLLNGCNARSLNFATSDNRPLYVIASDGGLLPEPVKVNELPVL
                     MGERFEVLVEVNDNKPFDLVTLPVSQMGMAIAPFDKPHPVMRIQPIAISASGALPDTL
                     SSLPALPSLEGLTVRKLQLSMDPMLDMMGMQMLMEKYGDQAMAGMDHSQMMGHMGHGN
                     MNHMNHSGKFDFHHANKINGQAFDMNKPMFAAAKGQYERWVISGVGDMMLHPFHIHGT
                     QFRILSENGKPPATHRAGWKDTVMVEGNVSEVLVKFNHDAPKEHAYMAHCHLLEHEDT
                     GMMLGFTV"
     gene            20246..20593
                     /locus_tag="J1Y83_001089"
     CDS             20246..20593
                     /locus_tag="J1Y83_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YacC family pilotin-like protein"
                     /protein_id="HBA7629257.1"
                     /translation="MKTFFRTVLFGSLMAVCANSYALSESEAEDMADLTAVFVFLKND
                     CGYQNLPNGQIRRALVFFAQQNQWDLSNYDTFDMKALGEDSYRDLSGIGIPVAKKCKA
                     LARDSLSLLAYVK"
     gene            20699..21565
                     /gene="speE"
                     /locus_tag="J1Y83_001090"
     CDS             20699..21565
                     /gene="speE"
                     /locus_tag="J1Y83_001090"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HBA7629258.1"
                     /translation="MAEKKQWHETLHDQFGQYFAVDNVLYHEKTDHQDLIIFENAAFG
                     RVMALDGVVQTTERDEFIYHEMMTHVPLLAHGHAKHVLIIGGGDGAMLREVTRHKNVE
                     SITMVEIDAGVVSFCRQYLPNHNAGSYDDPRFKLVIDDGVNFVNQTNQTFDVIISDCT
                     DPIGPGESLFTSAFYEGCKRCLNPGGIFVAQNGVCFLQQEEAIDSHRKLSHYFSDVGF
                     YQAAIPTYYGGIMTFAWATDNDALRHLSTEIIQARFLASGLKCRYYNPAVHTAAFALP
                     QYLQDALASQPS"
     gene            21581..22375
                     /gene="speD"
                     /locus_tag="J1Y83_001091"
     CDS             21581..22375
                     /gene="speD"
                     /locus_tag="J1Y83_001091"
                     /EC_number="4.1.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine decarboxylase"
                     /protein_id="HBA7629259.1"
                     /translation="MKKLKLHGFNNLTKSLSFCIYDICYAKTTEERDGYIAYIDELYN
                     ANRLTEILSETCSIIGANILNIARQDYEPQGASVTILVSEEPVDPKLIDKTEHPGPLP
                     ETVVAHLDKSHICVHTYPESHPEGGLCTFRADIEVSTCGVISPLKALNYLIHQLESDI
                     VTIDYRVRGFTRDINGMKHFIDHEINSIQNFMSEDMKALYDMVDVNVYQENIFHTKML
                     LKEFDLKHYMFHTKPEDLTDSERQEITAALWKEMREIYYGRNMPAV"
     gene            complement(22413..22775)
                     /locus_tag="J1Y83_001092"
     CDS             complement(22413..22775)
                     /locus_tag="J1Y83_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414661.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YacL family protein"
                     /protein_id="HBA7629260.1"
                     /translation="MDYEFLRDITGVVKVRMSMGHEVVGNWFNEEVKENLALLDEVEQ
                     AAHALKGSERSWQRAGHEYTLWMDGEEVMVRANQLEFAGDEMEEGMNYYDEESLSLCG
                     VEDFLQVVAAYRNFVQQK"
     gene            complement(22950..25547)
                     /gene="acnB"
                     /locus_tag="J1Y83_001093"
     CDS             complement(22950..25547)
                     /gene="acnB"
                     /locus_tag="J1Y83_001093"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aconitate hydratase
                     2/2-methylisocitrate dehydratase"
                     /protein_id="HBA7629261.1"
                     /translation="MLEEYRKHVAERAAEGIAPKPLDANQMAALVELLKNPPAGEEEF
                     LLDLLTNRVPPGVDEAAYVKAGFLAAVAKGEAKSPLLTPEKAIELLGTMQGGYNIHPL
                     IDALDDAKLAPIAAKALSHTLLMFDNFYDVEEKAKAGNEYAKQVMQSWADAEWFLNRP
                     ALAEKLTVTVFKVTGETNTDDLSPAPDAWSRPDIPLHALAMLKNAREGIEPDQPGVVG
                     PIKQIEALQQKGFPLAYVGDVVGTGSSRKSATNSVLWFMGDDIPHVPNKRGGGLCLGG
                     KIAPIFFNTMEDAGALPIEVDVSNLNMGDVIDVYPYKGEVRNHETGELLATFELKTDV
                     LIDEVRAGGRIPLIIGRGLTTKAREALGLPHSDVFRQAKDVAESDRGFSLAQKMVGRA
                     CGVKGIRPGAYCEPKMTSVGSQDTTGPMTRDELKDLACLGFSADLVMQSFCHTAAYPK
                     PVDVNTHHTLPDFIMNRGGVSLRPGDGVIHSWLNRMLLPDTVGTGGDSHTRFPIGISF
                     PAGSGLVAFAAATGVMPLDMPESVLVRFKGKMQPGITLRDLVHAIPLYAIKQGLLTVE
                     KKGKKNIFSGRILEIEGLPDLKVEQAFELTDASAERSAAGCTIKLNKEPIIEYLNSNI
                     VLLKWMIAEGYGDRRTLERRIQGMEKWLANPELLEADADAEYAAVIDIDLADIKEPIL
                     CAPNDPDDARPLSAVQGEKIDEVFIGSCMTNIGHFRAAGKLLDAHKGQLPTRLWVAPP
                     TRMDAAQLTEEGYYSVFGKSGARIEIPGCSLCMGNQARVADGATVVSTSTRNFPNRLG
                     TGANVFLASAELAAVAALIGKLPTPEEYQTYVAQVDKTAVDTYRYLNFDQLSQYTEKA
                     DGVIFQTAV"
     gene            25902..27659
                     /locus_tag="J1Y83_001094"
     CDS             25902..27659
                     /locus_tag="J1Y83_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3300 domain-containing protein"
                     /protein_id="HBA7629262.1"
                     /translation="MKMTLPFKPHVLALICSAGLCAASAGLYIKSRTVEAPVEAQSTQ
                     QTAPDISAVTLPATVSVPPVTPAVVKSAFSTAQIDQWVAPVALYPDALLSQVLMASTY
                     PTNVAQAVQWSHDNPLKQGDAAIQAVSDQPWDASVKSLVAFPQLMALMGENPQWVQNL
                     GDAFLAQPQDVMDSVQRLRQLAQQTGSLKSSTEQKIITTTKKVVPVNQPANAPATQSN
                     TVSTSSPVVAEPAPTVIAIEPANPDVVYIPNYNPNVVYGSWANTAYPPVYLPPPAGEP
                     FVDSFVRGFGYSMGVATTYALFSSIDWDDDDHDHHHHDDDDYHHHDGGHRDGNDWQHN
                     GDNINIDVNNFNRITGEHLTDKNMAWRHNPNYRNGVTYHDQDMAKRFHQTDVNGGMSA
                     TQLPAPTRDSQRHAAASQFQQRTHTAPVITRDTQRQAAAQRFNEAEHNGSYDDFRDFS
                     RRQPLTQQQKDAARQRYQSASPEQRQAAREKMQTNPQNQQRREAARQRIQSASPEQRQ
                     AVREKMQTNPQNQQQRDAARQRIQSASPEQRQVFREKVQESRPQRLNDSNHTARLNNE
                     QRSAVRERLSERGARRLER"
     gene            complement(27901..29325)
                     /gene="lpdA"
                     /locus_tag="J1Y83_001095"
     CDS             complement(27901..29325)
                     /gene="lpdA"
                     /locus_tag="J1Y83_001095"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HBA7629263.1"
                     /translation="MSTEIKTQVVVLGAGPAGYSAAFRCADLGLETVIVERYNTLGGV
                     CLNVGCIPSKALLHVAKVIEEAKALAEHGIVFGEPKTDIDKIRTWKEKVINQLTGGLA
                     GMAKGRKVKVVNGLGKFTGANTLEVEGENGKTVINFDNAIIAAGSRPIQLPFIPHEDP
                     RIWDSTDALELKEVPERLLVMGGGIIGLEMGTVYHALGSQIDVVEMFDQVIPAADKDI
                     VKVFTKRISKKFNLMLETKVTAVEAKEDGIYVTMEGKKAPAEPQRYDAVLVAIGRVPN
                     GKNLDAGKAGVEVDDRGFIRVDKQLRTNVPHIFAIGDIVGQPMLAHKGVHEGHVAAEV
                     IAGKKHYFDPKVIPSIAYTEPEVAWVGLTEKEAKEKGISYETATFPWAASGRAIASDC
                     ADGMTKLIFDKESHRVIGGAIVGTNGGELLGEIGLAIEMGCDAEDIALTIHAHPTLHE
                     SVGLAAEVFEGSITDLPNPKAKKK"
     gene            complement(29533..31425)
                     /gene="aceF"
                     /locus_tag="J1Y83_001096"
     CDS             complement(29533..31425)
                     /gene="aceF"
                     /locus_tag="J1Y83_001096"
                     /EC_number="2.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex
                     dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HBA7629264.1"
                     /translation="MAIEIKVPDIGADEVEITEILVKVGDKVEAEQSLITVEGDKASM
                     EVPSPQAGIVKEIKVSVGDKTQTGALIMIFDSADGAADAAPAQAEEKKEAAPAAAPAA
                     AAAKDVNVPDIGSDEVEVTEILVKVGDKVEAEQSLITVEGDKASMEVPAPFAGTVKEI
                     KVNVGDKVSTGSLIMVFEVAGEAGAAAPAAKQEAAPAAAPAPAAGVKEVNVPDIGGDE
                     VEVTEVMVKVGDKVAAEQSLITVEGDKASMEVPAPFAGVVKELKVNVGDKVKTGSLIM
                     IFEVEGAAPAAAPAKQEAAAPAPAAKAEAPAAAPAAKAEGKSEFAENDAYVHATPLIR
                     RLAREFGVNLAKVKGTGRKGRILREDVQAYVKEAIKRAEAAPAATGGGIPGMLPWPKV
                     DFSKFGEIEEVELGRIQKISGANLSRNWVMIPHVTHFDKTDITELEAFRKQQNEEAAK
                     RKLDVKITPVVFIMKAVAAALEQMPRFNSSLSEDGQRLTLKKYINIGVAVDTPNGLVV
                     PVFKDVNKKGIIELSRELMTISKKARDGKLTAGEMQGGCFTISSIGGLGTTHFAPIVN
                     APEVAILGVSKSALEPVWNGKEFVPRLMLPISLSFDHRVIDGADGARFITIINNTLSD
                     IRRLVM"
     gene            complement(31440..34103)
                     /gene="aceE"
                     /locus_tag="J1Y83_001097"
     CDS             complement(31440..34103)
                     /gene="aceE"
                     /locus_tag="J1Y83_001097"
                     /EC_number="1.2.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring),
                     homodimeric type"
                     /protein_id="HBA7629265.1"
                     /translation="MSERFPNDVDPIETRDWLQAIESVIREEGVERAQYLIDQLLAEA
                     RKGGVNVAAGTGISNYINTIPVEEQPEYPGNLELERRIRSAIRWNAIMTVLRASKKDL
                     ELGGHMASFQSSATIYDVCFNHFFRARNEQDGGDLVYFQGHISPGVYARAFLEGRLTQ
                     EQLDNFRQEVHGNGLSSYPHPKLMPEFWQFPTVSMGLGPIGAIYQAKFLKYLEHRGLK
                     DTSKQTVYAFLGDGEMDEPESKGAITIATREKLDNLVFVINCNLQRLDGPVTGNGKII
                     NELEGIFEGAGWNVIKVMWGSRWDELLRKDTSGKLIQLMNETVDGDYQTFKSKDGAYV
                     REHFFGKYPETAALVADWTDEQIWALNRGGHDPKKIYAAFKKAQETKGKATVILAHTI
                     KGYGMGDAAEGKNIAHQVKKMNMDGVRHIRDRFNVPVSDADIEKLPYITFPEGSEEHT
                     YLHAQRQKLHGYLPSRQPNFTEKLELPSLQDFGALLEEQSKEISTTIAFVRALNVMLK
                     NKSIKDRLVPIIADEARTFGMEGLFRQIGIYSPNGQQYTPQDREQVAYYKEDEKGQIL
                     QEGINELGAGCSWLAAATSYSTNNLPMIPFYIYYSMFGFQRIGDLCWAAGDQQARGFL
                     IGGTSGRTTLNGEGLQHEDGHSHIQSLTIPNCISYDPAYAYEVAVIMHDGLERMYGEK
                     QENVYYYITTLNENYHMPAMPEGAEEGIRKGIYKLETIEGSKGKVQLLGSGSILRHVR
                     EAAEILAKDYGVGSDVYSVTSFTELARDGQDCERWNMLHPLETPRVPYIAQVMNDAPA
                     VASTDYMKLFAEQVRTYVPADDYRVLGTDGFGRSDSRENLRHHFEVDASYVVVAALGE
                     LAKRGEIDKKVVADAIAKFNIDTDKVNPRLA"
     gene            complement(34264..35028)
                     /gene="pdhR"
                     /locus_tag="J1Y83_001098"
     CDS             complement(34264..35028)
                     /gene="pdhR"
                     /locus_tag="J1Y83_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex transcriptional
                     repressor PdhR"
                     /protein_id="HBA7629266.1"
                     /translation="MAYSKIRQPKLSDVIEQQLEFLILEGTLRPGEKLPPERELAKQF
                     DVSRPSLREAIQRLEAKGLLLRRQGGGTFVQSSLWQSFSDPLVELLSDHPESQYDLLE
                     TRHALEGIAAYYAALRSTDEDKERIRELHHAIELAQQSGDLDAESNAVLQYQIAVTEA
                     AHNVVLLHLLRCMEPMLAQNVRQNFELLYSRREMLPLVSSHRTRIFEAIMAGKPEEAR
                     EASHRHLAFIEEILLDRSREESRRERSLRRLEQRKN"
     gene            complement(35484..35774)
                     /locus_tag="J1Y83_001099"
     CDS             complement(35484..35774)
                     /locus_tag="J1Y83_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000282231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HBA7629267.1"
                     /translation="MYNFKDKIEDYTEREFIELLGEFTNPTGDNAQLKGEELDKYWDD
                     LEEHLTRITQHPLMSDLIYYPAKKGDDKPENILKIVKEWRRSQGLPLFKDSE"
     gene            complement(35775..36014)
                     /locus_tag="J1Y83_001100"
     CDS             complement(35775..36014)
                     /locus_tag="J1Y83_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020231867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA7629268.1"
                     /translation="MSQYPELIAQFSSGNQTRIKQGLIAKTPLEGWHYGPKEIVKKFH
                     MYHCVAIEYSGEIYDIDNLRRAHLICIMKFITGDS"
     gene            complement(36172..36465)
                     /locus_tag="J1Y83_001101"
     CDS             complement(36172..36465)
                     /locus_tag="J1Y83_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000471926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HBA7629269.1"
                     /translation="MFKEKLEDYTEEEFLNFLGGLRSTMKDGKPLKGKELEMYWDSLV
                     DHFIEITQHPSGSDLIFYPKSQGDDKPENILKIVKEWRRSQGLPLFKDSKQSR"
BASE COUNT         9330 a         9439 c         8665 g         9185 t
ORIGIN      
        1 atgtttagta aaattaactt ccatatgtca tgatattatg cagaatgtct gtccaatgat
       61 gaatgaatgt tatacttggt aaaaaacaaa cagacatcat taacaaagta acgttcagtt
      121 acatggcatt gcatcaaaat ctcatcatca tgcaatgctg ttatctgctt tatggaatat
      181 tgataaagga tttttatgtc taaaaaatta ggttttgtgc tgtccggtat tatgttggca
      241 atggctgctg gtactgcatt tgctgctaac gatatggacg gtggtcagtt aaatattagc
      301 ggcctggtcg ttgataacac ctgtgaagct cgtgttgatg gcggtaacaa agacggtctg
      361 atcctgctgc aaactgctac cgttgctgaa atcaccgatg gtgtactgga taccaccgta
      421 ggtgctaaag ctaaaccatt cagcattact attgactgta gcaaagctaa cccggctccg
      481 ggtactactg caaaaatgac cttcggttct gttttctttg gtaacagcaa aggaacactg
      541 aacaacgaca tgtctattaa caccccgtct gacggcgtaa atatcgctct gcacaatatt
      601 gaaggttcta ttattaaaca ggttcaggtc aataatccgg gtgatgttta cagcaaaact
      661 ctggattcaa cgtctaaatc tgcaacctac gattttaaag catcctatgt tcgtgcggat
      721 gcaaacaaag cagctactgc cggttatgta aaaaccaact cagcatatac cattacttac
      781 cagtaaatat caatcactta aattgatcgg gtgaatatat acagggaata acatcaaata
      841 tttttacttt tgataattat tccctgtttt aattaattct gtaaggaatg gtttatgttt
      901 tttaatacta aacataccgc agcattgtgc ttagcgagct gtatggcttt tagctcatca
      961 gctattgcag acatcgtaat ttcaggtaca cgcgtagttt ataaaagcga acaaaaaaac
     1021 gtcaatgtac gtctggaaaa taaaggcaat aacccattgc tggttcagag ttggttagat
     1081 actggcgatg acaacgctga accaggcagt attactgtcc cctttaccgc tacgccgcca
     1141 gtatcacgta ttgatgctaa acgtggccaa acaatcaaac tgatgtacac cgccagcagt
     1201 gctctgccaa aagacagaga aagtgtgttc tggtttaacg tactggaagt tccgccaaaa
     1261 ccagatgcag caaaagcagc aaatcaaagc ctgctacaac tggcatttcg cacacgtata
     1321 aaactgttct atcgcccgga aggattgagt ggaatgccat ctgatgcacc attggctctg
     1381 aagtggtcct gggcaacatc tgaaggcaaa gcagcattac gcgttgataa ccctacgccc
     1441 tactatgtct cttttagcag tggtgattta gaagccaacg gcaaacgcta ccccctggat
     1501 atgacaatga ttgcgccgtt cagtaacgac gtcatcaagg taaatgggat gagtggcaga
     1561 acaagctctg caaaagtgca ttttttcgcc attaatgatt ttggtggatc tattgaaggc
     1621 aacgccaacc tttaatctgg caaggatata acacagcagg aagcagaacg tgactattaa
     1681 atctactaat caccttaccc acatcgccac gttttgcgct ctgctgtata gcaatagtgc
     1741 tttatgcgct gaactcgtcg aatatgacca tacatttctg atgggaaagg atgcttcaaa
     1801 tatcgacctt agtcggtata cagaaggcaa tcccacacta cctggcattt acgatgtcag
     1861 tgtgtatgtt aacgatcaac ccatcatgag ccaaagcatt gcttttgctg ttattgaagg
     1921 taaaaagaac gctcaggcct gcattacgca gaaaaattta ttgtaattcc atatcagttc
     1981 accagacaag aatagcgaaa aagccatttt gcttaagcga gacgaagatc ttggtgattg
     2041 cctcaatctg gctgaaatga tccctcaatc atccattcgt tacgatgtta acgatcaacg
     2101 cctggatatc gatgtccccc aggcatggat tatgaagaat tatcaaaact atgttgatcc
     2161 ctctttgtgg gaaaatggca ttaatgcagc catgctttcc tacaacctca atggatatca
     2221 cagtgaaagt ccaggcagaa caaatgacag tatctatgcg gcattcaacg gtgggataaa
     2281 cttaggcgcg tggcggttac gtgcctcagg taactacaac tggatgacca atgtccatag
     2341 cgattacgat tttcagaatc gctatttaca acgcgatctt gcgtcactac gttcacaact
     2401 ggttattggt gaatcttaca cgaccggcga gaccttcgat tcagtcagca ttcgcggtat
     2461 tcgcttatac agtgacagtc gaatgctgcc tccggtatta gccagttttg cgccaatcat
     2521 tcatggcgta gcgaatacca atgccaaagt aaccgttatg caaaatggtt ataaaatcta
     2581 tgaaacgacg gtgccgccag gggcctttgc cattgatgat cttagcccgt caggttatgg
     2641 cagcgatctc attgtgacta tcgaagaagc tgatggcaca aaacggacgt tctcccagcc
     2701 attttcatct gttgtgcaaa tgctgcgccc tggcgttggt cgctgggata ttagcgccgg
     2761 tcaggtttta aaagacagta tccaggatga gcctaattta tttcaagcca gctactacta
     2821 tggtttgaat aactacctga caggatatac cggtattcag ctaaccgata ataactacac
     2881 cgccggattg ctggggcttg gtatgaatac ccccgtgggg gctttttctg tcgatgttac
     2941 tcactcaaat gtcagcattc cggacgataa aacctatcaa ggacaaagct accgtatttc
     3001 ctggaacaag ttatttgaaa ataccagtac atcattaaat atcgcggcct atcgctactc
     3061 cacccagcat tatctgggcc tcaatgatgc gctgacgctg attgatgaag tagaacatcc
     3121 tgagcaagat cttgagccta aatccatgcg caattactcg cgcatgaaaa atcaggtcac
     3181 ggtcagtatc aaccaaccgc tgaaatttga gaaaaaggat tacggttcat tttatctctc
     3241 gggaagctgg tcagattact gggcctccgg gcaaaacagt actaattact ctattggtta
     3301 tagcaatagt gcgtcctggg gcagttacag tatcagtgct caacgttcgt tgaatgaaga
     3361 tggtcaaacc gacgatagca tttatctcag cttcacgatt ccaatagaaa atttactggg
     3421 tacggaacac cgcagctccg gcttccagag tattgataca caattaaaca gcgactttaa
     3481 gggtaataac caactcaata tcagcagtag tggttacagc gataccaatc gcatcagcta
     3541 cagcgtaaac acgggttata tgatgaataa atccagtgat gatttgagtt atatcggcgg
     3601 ttatgccagt tatgaatcgc cgtggggaac gctgtcaggt tcagcctcag caagcagtga
     3661 taatagtcgc caattctcac tcaatactga tggtggattt gtgttacata gcggcgggct
     3721 gacgttcagt aatgacagtt ttagtgattc cgacactctg gctgtcattc aggcgccagg
     3781 agcaaaaggc gctcgtatta actacggaaa cagcaccgta gaccgctggg gttatggtgt
     3841 gaccagcgcc ctttcgccat accatgaaaa tcgtatcgca ctggacatta acgatctgga
     3901 aaacgatgtt gaattgaaaa gcaccagtac tgtcgccgtc ccgcgccagg gagccgtcgt
     3961 ctttgctgat tttgaaacag ttcagggaca atcggcaatt ctgaatatcg tgcgaagcga
     4021 tggtaaaaat attccatttg cagcggatat ttatgatgaa caaaacaatg tcatcggcaa
     4081 cgtaggtcag ggtggtcagg cctttgttcg cggtatcggg caggaaggaa atatacgaat
     4141 tacctggatt gaagagggca aaccggtcag ttgttttgct cattaccaac aaaacacgac
     4201 atcagaaaaa atagcacaat ctattattct gaacggactt cgttgtcaga tacagtaacc
     4261 acaagggact tccaatgata aaaaccacac cacataaaat aacaatactc atgggactat
     4321 tgttatcgcc ttcggtattt gcaacggatg ttaatgtcga ttttaccgct actgtgaaag
     4381 cgacaacatg taacatcacc ctgacatcgc tgaatggttc aaacgtcgtt gataatggca
     4441 atgatagtta taccctgata atccccaata tgggactgga taaaatagtc aataaagcgt
     4501 cacaatctga ggccaacttc aaattagtcg ccagtgggtg tagcagtgga attagctgga
     4561 ttgataccac aatgactgga aatcagtctg gtagtacatc gctgattatt ccacaatctt
     4621 ctgactcctc ctcaacaacc agtaatattg gtatgggatt taaaagatta gagacttcgg
     4681 gcgatacgtt tctgaaaccc aatagcgcgg aatacatccg ctggaaaacc acagaaatta
     4741 gcaccagtgg tctggaaatg actgtcgcac tgcgcgaaat aaccccagga cagggcatac
     4801 ccgggaaatt tcgcgcacag gcaacgttca atttcagcta tcaataacaa ggatgacaat
     4861 gatgatatta aaaaaattat gttatggatt atcgggtagt ttatttctgg cttcatcact
     4921 gttaaccatt tcctcacatg cggccactga tagtattggc ctgactgtta ctactgacgt
     4981 agaaatgggt acctgtacat caacattgac tgatgacggt acagctaaac tgtccaccat
     5041 caatttcggt gatgtatata tttctgaaat taatgccgaa actaaaatta aaaccttcaa
     5101 attacagttc aaagactgtg cggggattcc cgggaaaaag gccaaaataa aattgactcc
     5161 acgagcgtta tgtgaaggaa attctaataa tggtcctgga tttgctaacg catcaacggc
     5221 agcagcaaaa gcagcggcgg tcgccgttga ggtctggagc acatctacac caggtaagaa
     5281 cggcgctaaa caattcagtt gtgtcacccc cgcgacagaa gaagtcagta ttgctaatgc
     5341 gacaggaagt aatgttgtcg attacccaat gagtgcggtg ttggtggtcg caaaagataa
     5401 aactgtcaca gatgtcaccg caggtgattt cactgctcct gcaacattta ctgtgactta
     5461 taactaacag gcagaccgat tcaggaacta aatatgcgcc ccactcaacg taatctgatg
     5521 aaaaaatcaa ttctgattct gtcgttgcta ttcagcgcca cttcccctgt ggttgcagga
     5581 caggatgttg acattgttgc aaatataaaa aataacacct gccaaagtgg aatcagcaac
     5641 aacggaaata ttgatttagg tgtcgttggc gtgggcgatt ttaccggaaa catttcagcc
     5701 gaaaattatc accccggtgg aaaagagttc actattacag tacaggactg cacacttcag
     5761 ggcacaggga atgtgttaaa tcagttacat attgattttc gtgcactcag tggcgtaatg
     5821 gcgacaggtt ccagtcaaat attcgccaat gaagacaata ccggggctaa aaatgttggg
     5881 gtagttatat tctctattca ggacccggca aataccttca acgttctcgg cgccactggt
     5941 agctcacgtt ccgtttatcc ggtcatgtca tcagcactaa acaattcatc ctggaaattt
     6001 agtacccgaa tgcaaaaaat cgatccaaca ctgaatgtca cctctggccc actgataagc
     6061 catgtgttag tggatatcta ctacgaataa cagacaaagt gctacaagta ttatcttgca
     6121 gcatgacaat ggagtaaaaa tgaagacatt cttcagatac tttctttttt tagcactatg
     6181 ctcttgttgt tatacagcaa gtgccggcac cgatgataac gtcagctatc ttgttgggaa
     6241 tgattacgga gtgggtccgg gagaccaaaa gtggagagaa acaggcccca gtgcagacat
     6301 tactgtcaaa ttcaggtatt caacaagcac aaataactta gttttttata aaccaaccca
     6361 actcggacca acaggtgtgt cattatactg gtctcagtta aatagcgctt caggtggagg
     6421 ttacctttac tgcaacagaa gtaatactgc aagcggtgag cctatggcaa ttgaaaatca
     6481 aatggttgat tcgggtaaac gttatggcgg gcataaacta tttgaaacat ctgtcccagg
     6541 cctgtattat acattaaaga tatcaaacat ttggtcatca tacaccacaa ccaatatcag
     6601 ttcatcaggt atgtatatcg gcgattctcc aactcaatat tttcggtgga tccccgaaag
     6661 tgaagccact ctatattggt cttgtaacaa tgcaaactca tctaataaat actgggccgt
     6721 aggtggagtt tggcagtcgc tcacggtgga attttatacc gataccaact ttgaccccac
     6781 aaagacaaac aaggtaacat tatctaagcc gggtggttat ctctactcat tcaaaggata
     6841 tggtcttggt ggcggtatca actatcacag ctatttcctt aaaatagatt ttgacctgga
     6901 taatatgacg ttatccaacc caacgtgttt tactgctgca cttactggcc catctgtaag
     6961 cggttcaaca gttaaaatgg gagattatac cccagcgcaa ataaaaaatg gcgcaacagc
     7021 ggttcctttt gatatcacgc tacaaaactg tattcgtgtt cgcaatatag aaacaaaact
     7081 tatgtcaaac aaggtgggca gtgtaagcaa agaacttctg gctaacacac tcacaggaaa
     7141 taacgcagca aaaggcgttg gagtactgat tgaaggttta gaaaacacta aaagtgcaca
     7201 aatggtactg aaaccaaatg attcaacatc tatttataaa gattatgaaa ctgaagatga
     7261 cactaccggg gggatttacc cggataaagg caacggtacc tcacaacctc ttcatttcca
     7321 ggctacatta aaacaagacg gtaatattgc tatagaaccg ggggatttta aagccaccag
     7381 cacttttcag gttacctacc cctgataaca ctatcgcacc gccagacaac ggcggtgcga
     7441 tacggataaa tcattgcccc caccaatgtg actgataaaa tgcgagacag cacaacatca
     7501 tttatcagga tacgctatga aaccaaccac gattgcttca ttgcagaaat gcaagcagga
     7561 taaaaagcgt tttgcgacca tcaccgctta cgactacagc ttcgctaaac tgtttgccga
     7621 agaaggactt aacgtcatgc tggtaggcga ttcactgggc atgacggttc aggggcatga
     7681 ctccacgcta ccggttaccg ttgctgatat cgcctatcac accgccgctg tgcgccgcgg
     7741 tgcgccaaac tgccttctgt tagcagacct gccatttatg gcgtatgcca cgccggaaca
     7801 agcctttgaa aacgccgcaa cggtaatgcg tgccggagcc aacatggtca aaattgaagg
     7861 cggtgagtgg ctggtggaaa ccgtacaaat gctgaccgaa cgtgccgttc cagtttgtgg
     7921 tcatttaggc ctaaccccac agtcagtgaa tattttcggt ggctacaaag tccaggggcg
     7981 cggcgatgaa gcgggcgatc gactgctcag cgatgcatta gccttagaag ctgctggggc
     8041 acaactgctg gtactggagt gcgtgccggt tgaactggca aaacgcatta ccgaagcact
     8101 ggcgatcccg gttatcggta ttggcgcagg caacgtcact gacggacaaa tcctcgtgat
     8161 gcacgatgcg ttcggcatta ctggcggtca tatccctaaa tttgccaaaa atttcctcgc
     8221 cgaaacgggc gacatccgcg cggctgtgcg gcagtatatg gctgaagtgg agtccggcgt
     8281 ttacccgggc gaagaacaca gtttccatta aggagtgacg ttgtgttaat tatcgaaacc
     8341 ctgccgctgc tgcgtcagca aatccgccgc ctgcgtatgg aaggtaagcg cgtggcgctg
     8401 gtgcccacca tgggtaacct gcacgacggc catatgaagc tggtcgacga agccaaagcc
     8461 cgcgccgatg tggtcgtcgt cagtattttc gttaacccaa tgcagtttga ccgcccggaa
     8521 gatctggcac gttatccacg gaccttgcaa gaggactgcg aaaagctgaa caaacgtaaa
     8581 gtggatttag ttttcgcgcc ttcggtaaaa gagatctacc cgaacggtac agaaacccac
     8641 acctatgttg atgttcccgg tctttccacc atgctggaag gtgccagccg tccgggacat
     8701 tttcgcggcg tttcgactat tgtcagcaag ctgttcaacc tggtccagcc agatatcgcc
     8761 tgcttcggtg aaaaggattt tcagcaactg gcgcttatcc gcaaaatggt tgctgatatg
     8821 ggctttgata ttgagattat cggcgtgcca attatgcgcg ccaaagacgg tctggcgctg
     8881 agttcccgta acggttatct gacggcggaa caacgcaaaa ttgcgccggg tctctacaag
     8941 gtcttaagtt caattgccga caaattgcag gctggcgaac gggatctcga tgaaattatt
     9001 gctattgccg ggcaagaact gaatgaaaaa ggtttccgtt ccgatgatat tcagattcgt
     9061 gatgccgaca cattgctgga agtctctgaa aacagcaaac gcgcagtgat tctggtagcc
     9121 gcctggcttg gcgatgctcg cctgatcgac aacaaactgg tcgaactggc gtaatgctta
     9181 actggcgcta cggctgatgg cgccagttat tcattgccac gttatctgta ggcaattatt
     9241 cataatgcag acaacttagt taatcatttt atcaatctca gcaagaggta aatttgccat
     9301 ctctgcaacg tcttcccgag acattccttt actcagaaga cgcagggcga attcctgact
     9361 tacttcctgt cttccctgct gaatcccctt ctcttcaaac cactgcgcca gcgtcatcat
     9421 agactcccct cccgtttccc tgtctctcac cacaccataa aacaaatccg cttgttcagt
     9481 atgaccgcgt tgcagcatat agttttgcat ggcaactaac tgacttccgc tagtgtaccc
     9541 ttcgtcaatc agcgtgacca gttgctccag caataacatt aagtcgcgct ggcgaatatg
     9601 tttttgcagc agttcgagaa tcgcaatccg ccgatgttgc atgatttcgt catccggtgt
     9661 gatagtgata tccaccagcg ggaaaggact gttatagacg cgtcgcgcca gttccggtga
     9721 gtaaaacata tcaaaccagc acattgatag cggataaggt gtggcctcgc cctgataaaa
     9781 cagaattggc accaccagcg gcagcttatc gtgatcagcc tccagatgcc ggtgcatggc
     9841 agcaatagaa taacgcatca tgcgaaaggc cattttctta tctggcttgc tttggtgttc
     9901 aatcacaaca tgcagataac ccgtactacc ctgcatctgt acggaataga gcacgtcagt
     9961 gctgtgccct ttcaggcttt cttcaataaa actccccgac tcaagatgca gcgtgtcgag
    10021 gtcacacagt tcgcgtagct ccgctggcaa atggatatcc agaaagtcgc gagccgtttc
    10081 cgcatgcatt aaaaattgtt taaacaccgc atcatgcggt gtggtactcg gtgcatccat
    10141 cttccctgcc ttccgtgtgg tcttgcgtga aggcagtatc cagtcatggt gtgccgtcgt
    10201 cagccgcctc tttagtgttt accgagcagc gttcagataa atttcaattg gttacaggcg
    10261 ttacatggca cgcttcggca cggtacgcaa actgctgctg caaatgccgt cagatgagta
    10321 gacactaaac aaaaatcggg caatactgcg tgagaatttc ctcaaagcag gcatcgcctg
    10381 cttcgttaac gacagggtag aaaggtagaa gttatgattc gcacgatgct gcagggcaaa
    10441 ctccaccgcg tgaaagtgac tcatgcggac ctgcactatg aaggttcttg cgccattgat
    10501 caggattttc ttgacgcagc cggtattctc gaaaacgaag ccattgatat ctggaatgtc
    10561 accaacggca agcgtttctc cacctatgcc atcgcggcag aacgcggttc gagaattatt
    10621 tctgttaacg gtgcggcggc ccactgcgcc agcgtcggcg atattgtcat catcgccagc
    10681 ttcgttacca tgccagatga agaagctcgc acctggcgac ccaacgtcgc ctattttgaa
    10741 ggcgacaacg aaatgaaacg taccgcgaaa gcgattccgg ttcaggttgc ctgattgtta
    10801 tccctgcggc tggttactca ccagccgcga catcgtctcc agcgaatccg ttcttaagat
    10861 ataaagtcgt tttagtaaca acggattatc ccccggtttt actttgcctt tcatggtcgt
    10921 caccgcgagg tgaaatccgg cttcttttgc tgccttcacg gctttgtcat taaatccgcc
    10981 aaatggataa gaaagataca aaatatgtgg attaaattgc gccaaagcgc ggcgtgaacg
    11041 tgcaaaatca aacagaatat tgtgctcact acggctcagc aatataggcc gccgataacc
    11101 atctatccga tgcaaaaaat gagtatgtga ctggaaatca aatacatcgc gaatttcgtt
    11161 aagttcagaa acgctcataa attgcagcga ttttgggttc catttctgcg ggtgacgttt
    11221 gatgcgcgag gtaacaataa acgccgtcgc cttcatacca tattgtttca acacaggata
    11281 cgcatagcgg ctcaccgact tgaggccatc atcaaaggta attaccaccg ctcgcgcagg
    11341 gagattgatc ttattcttca cgtaaccttc cagctgcgcc atgctcagtg tcgcgtatcc
    11401 cctgtcgcgc agccaggcca tctggttatt gaaagcgcgt accgaggtgg tcgtcgaagt
    11461 atggcgaaaa cgggtgtttt cttcgtcgcg caaaatatgg tgataggtta gcactggcag
    11521 gccattatcg ggttgggcat ccagcgcgct gatataggcc aatcgatcgc caatacggat
    11581 ctgataccag gtttgattta acctgtcttt cagtttatgc aaaatcgggt agcgcaaatt
    11641 gtccgccagt actccaaatg gcgcactgcc cacactcggc gcgttataaa ccggcgtatc
    11701 tttccaggtt attaagttct gattactcag cggcttgttg aggtcaccta aaccatcttc
    11761 aactttttgt cgcccctgaa ccggctcgag atggccttta tcgataaacc ctttaccaaa
    11821 gccaaaatta aatacgtaat aacttgcggc agtgggctcc acggcaatga tttgtccggc
    11881 ccgaatattc cctaccgtca ccattttgtc gccaatttgc gcccagaccg cggcattttc
    11941 gatggtttgc atataacggg caggtaacgc ggcactgaca cttgcggtaa acagcatcag
    12001 caggagaata acagcttgtt tgtacatgaa aaaataaccg agggtgaaac aacgctttta
    12061 ttgtagcaaa agcgtcatca gaatcgcggg ttaatactca tacaaattat gtttgaggac
    12121 gaaaggcgga tttttttgtt gttggtgcca aagactactc acctccggcg cacccagttc
    12181 gacaatatgc tcgcgaaacg ctgaactggt catggtttca cggcaagcca tcatctgttc
    12241 aattaacggt aacgtgacac cagaaatcac ttcgcaacgg gagtgtttgt ggcttaataa
    12301 tgaagccacg cggtacggtg gcgctccggc tatatcggtt aaaaagataa cgccctcacc
    12361 agagtccgtt tcatgcaagg catcgcacat catgcggctg agcatattag agcttaatcc
    12421 gcgccagaaa ttcacggccc ggcactgaag aagtgcccca tgttttttct ccagcgcatc
    12481 cagtagctct tgcgcccggt catcgtgaca ggtaattacc caacctaaca tcgccttcct
    12541 ccttagccga aaatcagtct accctgacca ttgttgataa agggtgatag ccgtcaaaac
    12601 tcctctcccc caaatccaga ggagaggaaa tagccttagc tacgcaaacc acgtccacgt
    12661 tggatcagcg accaacagat caaataaaac gccacaataa agaccaccag tacgccaaag
    12721 gtagtgacca gcggaacatc attgataccg aggaagccgt agcggaaacc actgatcata
    12781 taaacgattg ggttcaggtg cgacagccct tgccagaacg gcggcaacaa agtcagggag
    12841 taaaacaccc cgcccaaata cgtgagtggc gttaacacaa aggttggcac caggctgatg
    12901 tcatcgaacg ttttggcaaa cacgccgttc agcaaacccg caagggagaa tagcaccgca
    12961 gtgagcacca gggttaaggc aacgaatacc cacgaatgca cctgaaacgg cacaaaaaac
    13021 agtgaaattg ccgtcaccaa aataccaaca aacagaccac gcgccacgcc accaccgaca
    13081 tatccggcaa taatgacatg agtcggaacc ggcgctacca gcagctcttc aatattacgc
    13141 tggaacttgg caccaaaaaa tgatgacgcg acgttagcgt aggcgttggt gatcaccgac
    13201 atcatgatca gccccggtac gatgaactgc atgtagctga agccatgcat atcgccaata
    13261 cgcgaaccaa tcaggttacc gaagataata aagtaaaggg tcatggtgat gactggcggc
    13321 accagcgtct gcacccagat acgcataaag cgatggatct ctttcgccca gatgcttttt
    13381 agcgccaccc agtaaagatg catcatgtgc gatctccttg tttttcatta accagtgaaa
    13441 caaacagctc ttccagacgg ttagctttgt tacgcatact taatacctga atgccctgct
    13501 cacttaactg cgtaaatacg ctgttgatgc cctgctcacg cagcacttca acttccagcg
    13561 tggcggtatc taccagtcga tactgatagc catcgagctt cggtaacgga cttttcggtg
    13621 cgagatcgag aataaaggtt tctgatttca gcttcgccag cagcgccttc atcgaagtat
    13681 tttccaccaa ctcaccgtgt tgaataatgc cgatattgcg gcacagcatt tctgcttctt
    13741 ccaggtagtg tgtggtgaga atgatggtgg tgcctttgtc gtttaaatcc ttcaaaaagc
    13801 cccacattga gcggcgaagt tcaatatcca cgcctgcggt cggttcgtcg agaatcagta
    13861 gtttaggttc atgcattaac gcacgggcaa tcattaaacg gcgcttcatc ccgccagata
    13921 acatacgcgc acgttcgttg cgttttcccc atagatcgag ttgtttaaga tacttttcgc
    13981 tgcgaatgta cgcttctttg cgctccacac cgtagtaccc tgcctgattc accacaattt
    14041 gctgcacggt ttcaaacggg ttgaagttaa attcctgtgg caccagcccc aactgacgtt
    14101 tagcgtttac gacatctttc tcgagatcgt aaccaaatac gctgacccgc ccggaggttt
    14161 tatttaccag agagctgata ataccgatag tggtcgattt tccggccccg ttcggcccga
    14221 gaagcgcata aaaatcaccc gcttcgacct gcaaatcgat tccacgaagc gcctgaacgc
    14281 cgcctggata ggttttttta agctgttgaa gttccagtgc aatggtcata aatttttact
    14341 taccttacgt tcttacactt tatctgtggt ttaaatcgtc ccggagttgc cctatattag
    14401 ccaaacgtaa ttatttggtt acaggtcgtt aacctccatg aaagacatag atacactcat
    14461 cagcaacaat gcactatggt caaaaatgct ggtggaagag gatcccgggt tttttgagaa
    14521 actggcacaa gcgcaaaaac cgcgctttct atggattgga tgttccgaca gtcgcgttcc
    14581 tgcagaacgt ttaaccggtc ttgagccggg cgaactcttt gttcaccgta atgttgctaa
    14641 cctggtcatt cacaccgacc tgaactgcct ttccgtggtt cagtatgcag tggatgtact
    14701 cgaagttgaa cacattatta tctgtggcca ctacggttgc ggcggcgtac aagccgcagt
    14761 tgaaaaccca gaactggggc ttatcaacaa ctggctgctg catatccgcg atatctggtt
    14821 caaacatagc tcattgctcg gcgaaatgcc gcaagagcgc cgtctggata ccttgtgtga
    14881 actgaacgtc atggaacagg tgtataacct gggccactcc accattatgc aatcagcgtg
    14941 gaaacgcggg cagaaagtta ccattcacgg ctgggcctac ggcattcacg acggcttgct
    15001 gcgtgatctg gatgtcacag cgactaaccg cgaaaccctt gagcaacgtt accgtcacgg
    15061 gatctccaac ctcaagctga aacacgccaa ccacaaataa aaatgccatg ccggatgcaa
    15121 cacatccggc aacttcacac ttactcgtcc agcagaatca ctttgccgat atacggcaga
    15181 tgacggtaac gctgtgcgta atcaatgccg taacccacca caaactcatc cgggatcgag
    15241 aaaccgataa attctaccgg gacgttcact tcacgacggg acggtttatc cagcagcgta
    15301 caaatcgcca acgacttcgg ttcgcgcagg cttaagatct cacgcacttt cgacagtgta
    15361 ttccccgagt cgatgatatc ttcaacaatc agcacgtcct tgccacggat atcttcatcc
    15421 agatctttga ggattttcac atcacgggtg gtggacatgc cgctaccgta gctggaggcg
    15481 gtcataaagt cgacttcatg agatacctga acttcacggc acaggtccgc cataaacata
    15541 aatgagccac gcagcagacc caccagcacc atatcgctgc cgctgtcttt gtaacgctca
    15601 gtaatctgac gacccagttc ggcgatacgc gctttaatct ccgcttcggg gatcattact
    15661 tctacagtat gtttcatatc tataaccata tgatttaaaa acaaatcact tcgcacgggc
    15721 aggttttggc accgcaacaa agcgcaagac acgcagtata ccagtaaaac tattcattcg
    15781 cggaatctgt taataaagct cgaattgtga tgacgatcac acatgttaaa acctataatt
    15841 aattgctact aaaatattaa tgaattgaaa tggtgtctct ttatggcaat taacaatacc
    15901 ggctcgcggc gattactcgt cacgctaaca gccctttttg cagcgctttg cgggctgtat
    15961 ctactcattg gcggaggctg gctggtcgtg attggcggct cctggtacta ccctatcgct
    16021 ggccttgtga tgctcggcgt cgcctggatg ctgtggcgca gtaaacgcgc cgcgctttgg
    16081 ctgtacgcag ccctgctgct cggcactatg atttggggcg tctgggaagt tggtttcgac
    16141 ttctgggcgc tgactccgcg cagcgacatt ctggtcttct tcggcatctg gctgatcctg
    16201 ccgtttgtct ggcgtcgcct ggtcattcct gccagcggcg cagttgccgc actggtggtc
    16261 gcactgctga ttagcggtgg tatcctgacc tgggccggat ttaacgatcc gcaggaaatc
    16321 agcggcacct taagcgccga taccacgcca gcagaagcca tctcccccgt tgccgatcag
    16381 gactggcctg cctatggtcg caatcaggaa ggtcaacgct tttcaccact gaagcaaatt
    16441 aacgctgata acgtccacaa gctgaaagaa gcctgggtgt tccgtactgg cgatgtgaag
    16501 cagccgaacg atccgggcga aatcaccaat gaagtgacgc caattaaagt gggcgatacg
    16561 ctttacctgt gtaccgctca ccagcgcctg tttgcgctcg atgccgccag cggcaaagag
    16621 aaatggcatt acgatcctga actgaaaacc aacgagtctt tccagcacgt aacctgccgt
    16681 ggcgtctctt atcatgaagc caaagcagaa acggcctcgc cagaagtgat ggcggattgc
    16741 ccgcgtcgta tcattcttcc ggtcaatgat ggtcgcctga ttgcgattaa cgctgaaaac
    16801 ggcaagctgt gcgaaacctt cgctaataaa ggcgtgctca atctgcaaag caatatgccg
    16861 gataccaaac cgggtctgta tgagccgact tcgccgccga ttatcaccga taaaaccatc
    16921 gtgatggccg gttcagtcac cgacaacttc tcaacccgcg aaacgtctgg cgtgatccgt
    16981 ggttttgatg tcaacaccgg ggagctgctg tgggcttttg atccgggcgc gaaagatccg
    17041 aatgcgatcc cgtctgacga acataccttt acctttaact caccaaactc gtgggcacca
    17101 gcggcctatg acgcgaagct ggatctggtc tatctgccga tgggcgtcac cacgccagat
    17161 atctggggtg gtaaccgcac accggaacag gaacgttatg ccagctcgat tctggcgctg
    17221 aatgccacta ccgggaaact ggcgtggagc tatcagaccg ttcaccacga cctgtgggat
    17281 atggatcttc cggcacagcc gacgctggcg gacatcaccg ttaatggtca gaaagtgccg
    17341 gttatttacg ctccggcgaa aaccggcaac atttttgtgc tcgatcgtcg taatggtgaa
    17401 ctggtggttc cggccccgga aaaaccggtt ccccaaggtg ctgccaaagg cgattacgtg
    17461 acccctactc aacccttctc ggaactgagc ttccgtccga cgaaagattt gagcggtgcg
    17521 gatatgtggg gtgccaccat gtttgaccag ttggtgtgcc gcgtgatgtt ccaccagatg
    17581 cgctatgaag gcatttttac gccgccatct gaacagggta cgctggtctt cccgggtaat
    17641 ctggggatgt tcgaatgggg cgggatttcc gttgatccga atcgtgaagt ggcgattgcc
    17701 aacccaatgg cactgccgtt tgtttcgaaa ctgatcccac gcggtcctgg taacccgatg
    17761 gaacagccga aagatgccaa aggcacgggt acggaatccg gcatccagcc gcagtacggt
    17821 gtaccgtatg gcgtcacgct caacccgttc ctctcaccgt ttggtctgcc atgtaaacag
    17881 ccagcatggg gctatatctc agcattggat ctgaaaacca atgaagtggt gtggaagaaa
    17941 cgtattggta cgccgcagga cagtatgcca ttcccaatgc ctgtaccggt gccgttcaat
    18001 atgggtatgc cgatgcttgg cgggccaatc tccacggcgg gtaacgtgct gtttatcgcc
    18061 gctacggcag ataactacct gcgcgcttac aacatgagca acggtgaaaa actgtggcag
    18121 ggtcgtttac cagcgggtgg tcaggctacg ccaatgacct atgaagtgaa tggcaagcag
    18181 tatgtggtga tctccgcagg cggtcacggt tcatttggta cgaagatggg cgactatatt
    18241 gtggcttatg cgctgccgga tgatgtgaag taaggcttgc tcagattgct gacaacgtgc
    18301 gatttgttca tgccggatgc ggcgtgaacg ccttatccgg cctacaaaac tgacaacgtg
    18361 cgctttgttc gtgccggatg cggcgtgaac gccttatccg gcctacaaaa catttgcaaa
    18421 ttcaatacat tgcgcgtagg cctgataagc gtagcgcatc aggcagtttt gcgtttgtca
    18481 tcagtttcat gcccggagag atccgggcat atttccgaat acggtctttt tataccgtaa
    18541 accctaacat catccccgta tcttcatgct ccagcagatg gcagtgcgcc atataagcat
    18601 gttctttcgg tgcatcgtga ttaaacttca ccagcacttc gctgacatta ccttctacca
    18661 taacggtatc tttccagcct gcgcgatgcg tcgctggcgg tttgccattt tctgacaaga
    18721 tacggaactg cgtaccgtgg atatggaacg gatgcagcat catgtcgccc acgccagaga
    18781 taacccaacg ttcgtactgc cctttcgccg ccgcaaacat cggcttattc atatcaaacg
    18841 cctgaccgtt gattttgttg gcatggtgga aatcaaactt cccgctgtgg ttcatatgat
    18901 tcatattgcc gtgccccata tggcccatca tctggctgtg atccatcccg gccatcgcct
    18961 gatcgccata tttctccatc agcatctgca tccccatcat atcgagcatt gggtccatag
    19021 aaagttgcag cttgcgtacc gtcagccctt ccagcgaagg taacgcaggc aggctactta
    19081 atgtgtctgg caaagcacca gaggcactaa tggcaatcgg ctgaatccgc attaccggat
    19141 gcggcttgtc aaacggcgca atcgccatcc ccatctggct gaccggcagc gtcaccaggt
    19201 caaagggttt gttgtcgtta acctccacca gcacttcaaa acgctcgccc atcagcactg
    19261 gcagctcgtt caccttcacc ggttcaggta gcagaccgcc gtcgctggca atcacataaa
    19321 gcgggcgatt gtcgctggtg gcgaaattga gcgagcgggc attacagcca ttgagcaaac
    19381 gcaggcgcag ccagccacgc ggggcagcgt gttgcgggta gattacaccg ttggtcagca
    19441 acgtatcgcc aaaccaaccc acagcggcgg tcatcacatc cagttgataa tcaatctgcc
    19501 cgtcagcgtt aaatttttta tcctgaacaa tcaccgggac atcatcaata ccccactgtt
    19561 ttggcagcat taatttcagg atctcgtcat cttcaatcac caccaaacca gccagcccca
    19621 tcgccacctg acgtccggtc ttgccatgtt gatgtggatg gaaccagcag gtagcggcag
    19681 gttgatcaac gttcaacgtc accgagcgct tgccacctgg cggaataatt ccctgcgggc
    19741 cgccgtccac ttcacccggt acttccagcc cgtgccagtg caacgtcgtc tcttccgtca
    19801 gttggttgta gatatcaacc gttaccgctt tgccgcgctg taatttcacc gccggcccca
    19861 gcagattgcc gttatagccc caggtagttg cggttttccc gccaaaggtg gactgacctg
    19921 cgccaatagt taactgaatg cgattacggg catcggtcgt gagcaaatca gggatcggta
    19981 acgttgggcg ttccgccgca aatactgcgc ggctccacag cggcaaggct gaagccacac
    20041 ccagcgcgac ggaatatttc aagaaatcac gacgttgcat agttatttcc ttattcttaa
    20101 gcaggcgaaa caaaatcaaa cgttgagcat agtccttccc cttacgggaa ggtcaagtcg
    20161 cagacacaat ttaatcgaca ataatggtcg tcgcctcgct cgcagtgtgc taacgtttat
    20221 cttctttaag cccctggtag aagcaatgaa gacgtttttc agaacagtgt tattcggcag
    20281 cctgatggcc gtttgcgcaa acagttacgc gctcagcgag tctgaagccg aagatatggc
    20341 cgatttaacg gcagtttttg tttttctgaa gaacgattgt ggttaccaga acttacctaa
    20401 cgggcaaatt cgtcgcgcgc tggtcttttt cgcccagcaa aaccagtggg atctcagtaa
    20461 ttacgacacc ttcgacatga aagccctcgg tgaagacagc taccgcgatc tcagtggcat
    20521 tggcattccc gtcgctaaaa agtgcaaagc cctggctcgc gattccttaa gcctgcttgc
    20581 ctacgtcaaa taatccctga tacctttttg tagaaatatt gaccgtgcat ccgcggtcaa
    20641 tgttagctat tatgttgcgc cattttttta cgggtgttaa caaaggaggt atcaacccat
    20701 ggccgaaaaa aaacagtggc atgaaacgct acacgaccag tttgggcagt actttgcggt
    20761 agataacgtt ctgtatcatg aaaagaccga tcaccaggat ctgatcattt ttgagaacgc
    20821 cgcatttggt cgcgtaatgg cgctggatgg cgtagtacaa accaccgagc gcgacgagtt
    20881 tatctatcat gagatgatga cccatgttcc gctactggcc cacggtcacg cgaaacatgt
    20941 gctgattatc ggcggcggcg acggtgccat gctgcgtgaa gtaacccgac ataaaaacgt
    21001 tgagtcaatc acgatggtgg aaatcgatgc gggtgtcgtg tcgttctgcc gtcagtatct
    21061 tcccaaccat aacgccggta gttacgacga tccgcgcttt aaactggtga tcgacgatgg
    21121 tgtcaatttc gttaatcaaa ccaaccagac ctttgatgtc attatctccg actgcaccga
    21181 tcctatcggt cccggcgaaa gtctgtttac ttctgcattt tatgaaggct gcaaacgttg
    21241 cctgaatcct ggcggtatct tcgtcgcaca aaacggcgtc tgctttttac agcaggaaga
    21301 ggccatcgac agccatcgca aactcagcca ttacttcagc gatgttggct tttatcaggc
    21361 ggcgatcccg acctattacg gcggcatcat gacctttgca tgggcgacag ataacgacgc
    21421 cttacgccat ctctcaaccg aaattattca ggcgcgtttt ctcgcttctg gcctgaaatg
    21481 ccgttattac aatccggcag tccatacggc agcttttgcc ttaccccagt atctgcaaga
    21541 tgcactggct tcacagccgt cctaaggaga agataagaaa ttgaaaaaac tgaaactgca
    21601 tggctttaat aatctgacca aaagtctgag tttttgtatt tacgatatct gctacgccaa
    21661 aactaccgaa gagcgcgacg gttatattgc ttatatcgat gaactctata atgccaaccg
    21721 tctgaccgaa atcctgtcag aaacctgttc cattatcggg gccaatattc ttaacatcgc
    21781 ccgccaggat tacgagccac aaggtgccag cgtcactatt ctggtgagtg aagaaccggt
    21841 cgacccgaaa cttatcgaca aaacagaaca ccccggccca ctgccagaaa cggtcgttgc
    21901 ccatctcgat aaaagtcata tttgcgtaca tacctacccg gaaagtcatc ctgaaggcgg
    21961 tttatgtacc ttccgcgccg atattgaagt ctctacctgc ggcgtgattt ctccgctgaa
    22021 ggcgctgaat tacctgatcc accagcttga gtccgatatt gtgactattg attatcgcgt
    22081 gcgcggtttt acccgcgaca ttaacggtat gaagcacttt atcgaccatg agattaattc
    22141 gattcagaac tttatgtccg aagatatgaa ggcgctgtat gacatggtgg atgtaaacgt
    22201 ctatcaggag aatatcttcc ataccaagat gttgcttaaa gagttcgacc ttaagcacta
    22261 catgttccac accaaaccgg aagatttaac cgacagcgag cgccaggaaa ttaccgctgc
    22321 gctgtggaaa gaaatgcgcg agatttatta cgggcgcaat atgcccgctg tttaacgtct
    22381 caggcggagc acggaggctc cgcctgattt acttacttct gctgcacgaa attacggtaa
    22441 gccgccacca cctgaagaaa atcctcaacg ccgcacagcg acaagctctc ttcatcgtag
    22501 tagttcatcc cctcttccat ttcatcgcca gcgaattcca gttgattggc gcgaaccatc
    22561 acctcttcac catccatcca tagcgtgtat tcatgccctg cccgttgcca ggaacgttcg
    22621 ctacctttca atgcgtgcgc cgcttgttcc acttcatcaa gcaaggccag gttttctttc
    22681 acctcttcat taaaccagtt cccgaccact tcatgcccca tggacatgcg cacctttacc
    22741 actccggtaa tatcgcgcag aaattcgtaa tccatagtgt gttcctctgc tcctggcaat
    22801 gcgccgaaat cgcattgcgc cattgatgta attatcgcag ccttaccgga gaaaaacagc
    22861 ggaacggcga tggtttagaa ataaaaaatg cccgggaatc tcattcaccg ggcattgtgt
    22921 cgtttatgcg cagcgcgtgc gcttacttat taaaccgcag tctggaaaat caccccgtcc
    22981 gctttttcgg tgtactgaga aagctggtcg aagttcagat aacggtaagt atcaacggct
    23041 gttttatcta cctgcgccac gtaggtctgg tactcttccg gcgtcggcag tttgccaatc
    23101 agcgccgcaa cagcagccag ttccgcagaa gccaggaaga cattcgcgcc agtacccaaa
    23161 cggttcggga agttacgggt agaggtggaa accaccgtcg caccgtctgc tacacgcgcc
    23221 tggttaccca tacacaggga acagccaggg atctcgatac gagcaccact cttaccgaag
    23281 acgctgtagt agccttcttc ggtcagctgc gcagcgtcca tacgcgttgg cggtgccacc
    23341 cacaggcggg tcggcaactg acctttgtgc gcatccagca gtttaccagc agcacggaag
    23401 tgaccgatgt tagtcatgca agaaccgata aatacttcgt cgatcttctc gccctgtacc
    23461 gcagacagcg gacgtgcgtc gtccgggtca ttcggtgcac agaggattgg ctctttgata
    23521 tccgccagat cgatgtcgat cactgccgcg tattccgcat ctgcatcggc ttccagcagc
    23581 tcaggattcg ccagccattt ttccatgccc tgaatacgac gctccagggt acgacgatcg
    23641 ccgtaacctt ccgcgatcat ccacttcagc aggacgatgt tagagttcag gtattcaatg
    23701 atcggttctt tgttcagctt gatggtacaa ccggcagcag aacgctcggc agacgcatcg
    23761 gtcagctcaa atgcctgctc aactttcaga tccggcagac cttcaatttc cagaatgcgg
    23821 ccagagaaga tgtttttctt acctttcttc tcaacggtca gcagaccttg tttgattgca
    23881 tacagcggga tcgcgtgtac cagatcgcgc agggtgatac ccggctgcat tttgcctttg
    23941 aagcgcacca gaacggattc tggcatatcc agcggcatta cgcctgttgc ggctgcaaat
    24001 gccaccagac cagaacccgc cgggaaagag ataccgatcg ggaaacgggt atgggagtca
    24061 ccaccggtac cgacggtatc cggcagcagc atacggttca gccaggagtg gattacgccg
    24121 tcacccggac gcagcgacac accgccacgg ttcataatga agtccggcag cgtgtggtgc
    24181 gtgttcacgt caactggctt cggatacgcc gcggtgtggc agaaagactg catcaccagg
    24241 tcagccgaga agcccaggca cgccaggtct ttcagttcat cacgggtcat cgggccggtg
    24301 gtgtcctggg aacctacaga agtcattttc ggttcacagt acgcgcccgg acgaatgcct
    24361 ttcacgccac aggcacggcc taccattttc tgcgccagcg agaagccgcg atcgctctca
    24421 gcgacatctt tcgcctgacg gaacacatcg ctgtgcggca ggccaagtgc ttcacgcgct
    24481 ttggtggtca ggccacgccc gataatcagc ggaatacggc cgccagcacg cacttcatca
    24541 atcagcacgt cggttttcag ttcgaaggtt gccagcagtt cgccggtttc gtggttacgc
    24601 acttcacctt tgtacgggta aacgtcaatt acgtcgccca tgttcaggtt agagacgtcg
    24661 acttcgattg gcagtgcacc cgcgtcttcc atcgtgttaa aaaagattgg tgcaatttta
    24721 ccgccgaggc acaaaccacc gccgcgtttg ttcggcacat gtggaatatc atcgcccata
    24781 aaccacagaa cggagttcgt ggcggattta cgcgatgaac ccgtacccac aacgtcaccg
    24841 acgtacgcca gcgggaaacc tttctgttgc agagcttcaa tctgtttgat cgggccaaca
    24901 acaccaggct gatctggctc aataccttca cgggcgtttt tcagcatcgc cagcgcgtgc
    24961 agtgggatat ccgggcgtga ccacgcatcc ggtgccggag aaaggtcatc ggtgttggtt
    25021 tcgccggtca ctttgaagac ggtaacggtc agtttttcag ccagcgccgg gcgattcagg
    25081 aaccattcgg catccgccca ggactgcatt acctgcttcg catattcgtt acctgctttg
    25141 gctttctctt ctacgtcata gaagttatcg aacatcagca gcgtgtgaga aagtgctttg
    25201 gcagcgatcg gtgccagttt ggcatcatcc agcgcgtcga tcagcggatg aatgttgtaa
    25261 ccaccctgca tagtgcccag cagttcaatg gctttttccg gagtcagcag cggggattta
    25321 gcttcgcctt tggcgacagc agccaggaag cctgctttga cataggcggc ttcatcgacg
    25381 ccagggggaa cacggttggt taacagatct aacaggaatt cttcttcgcc cgcgggcggg
    25441 tttttcagca gctctacaag tgcggccatt tggtttgcat ccaggggttt gggcgcaatc
    25501 ccctcagcgg cacgctcagc tacgtgctta cggtattctt ctagcacgac ggttctcctc
    25561 gctctcattg tcatagtgcg gcaggcgatt ctcttcacgc tcctgtgaga cagcagtttg
    25621 tagggtaaat gcccaatacc gcaacgggca gaatagcagg attttgacga tgtgttaatc
    25681 tgtttacaaa aaagcaacat aaaaaagtgg ctgaatcgtt aaggatagtc tagagaatgt
    25741 ttccctccgt aaaaacttcg ttaattcgca tggtgataat cacctttctc aacgaatccc
    25801 aagattagtc aaaatttaaa ctacggcctc tttatactcg gattcacagc acctgcgggt
    25861 ggcagttcgc cgaccattgc gatttccttg agatccgaat tatgaaaatg actttgccgt
    25921 ttaaacccca tgtgctggcg ctaatttgca gtgccgggct ttgtgccgcc tctgccgggc
    25981 tatatataaa aagccgcaca gtggaagcgc ctgtggaagc gcaatcgaca caacagactg
    26041 cgcctgacat ctccgcagtt acgcttcctg caacggtttc cgtgcctcct gtaacacctg
    26101 ccgtcgtcaa atccgcattc agcactgctc aaatagatca atgggttgca cctgtcgcgc
    26161 tataccccga cgccctactt tcgcaggtgc tgatggcatc aacctatccg acaaacgttg
    26221 cgcaagcagt acaatggtcg cacgataacc cacttaaaca aggcgatgct gctattcagg
    26281 cggtatctga ccagccgtgg gacgccagcg ttaaatcact ggtggccttt ccacaattga
    26341 tggcattgat gggcgaaaac ccgcaatggg tgcaaaacct gggcgatgct tttctggcgc
    26401 agccgcaaga cgtgatggac tcggtacagc gattgcggca actggcgcaa caaaccggct
    26461 cgctgaagtc atcaacggaa caaaagatca tcacgacaac gaaaaaagtc gtaccggtaa
    26521 atcagcccgc caacgcccct gccacacaat caaataccgt ttctacgtcc agccccgtcg
    26581 tcgcagaacc tgcaccgacc gtaatagcca ttgagccagc caatccggat gtggtttata
    26641 ttccgaacta caacccaaac gtggtttacg gcagttgggc caataccgct tatccgccag
    26701 tttatctgcc gccgccagcc ggagaaccgt ttgttgacag ctttgtgcgc ggattcggct
    26761 atagcatggg cgttgctacc acatacgcac tattcagcag catcgactgg gacgacgatg
    26821 atcatgacca tcatcatcat gacgatgatg attatcatca ccacgatggc ggtcatcgtg
    26881 acggtaatga ctggcaacac aacggcgaca acatcaatat cgacgtcaac aatttcaacc
    26941 gtatcaccgg tgagcatctt actgataaga atatggcatg gcggcacaat ccaaactacc
    27001 gtaatggtgt gacctatcat gatcaggata tggcaaagcg gtttcatcaa accgatgtca
    27061 acggcggaat gagcgccacg cagttacctg ctccaacacg cgacagccag cgtcatgcgg
    27121 cagcaagtca gtttcagcaa cgaacacaca ccgcccccgt cattacgcga gatacccaac
    27181 gtcaggcagc ggcacagcgg tttaatgaag ctgaacacaa tgggagctat gacgacttcc
    27241 gcgacttcag ccgtcgccag ccactgaccc agcaacaaaa ggacgccgct cgtcagcgtt
    27301 atcagtcggc ttctcctgag cagcgccagg cggctcgcga gaaaatgcag actaacccac
    27361 agaaccagca gcgaagagag gcggcacgtc agcgtattca gtccgcgtcg cctgagcagc
    27421 gccaggcggt tcgcgagaaa atgcagacta acccgcagaa tcaacagcaa agagatgcag
    27481 cacgtcagcg tatccagtct gcctcgcctg agcagcgtca ggtttttcga gagaaagttc
    27541 aggagagccg cccgcagcgt ctaaacgaca gtaaccatac tgcccgattg aataatgagc
    27601 aacggtcagc ggtacgcgaa cggttgtctg agcgtggagc aaggagactg gaaaggtaaa
    27661 ttgcaggcgt aaaaaagcgg cgttgttagc cgcttaagac tgatgacaaa catcgaatcg
    27721 cccgatgcgc aagcttatcg ggcctacaca agccatgcaa tatattgaat ttgcatgatt
    27781 ttgtaggtcg gataaggcgt ttacgccgca tccggcatca accacgagca ctttatcaac
    27841 aatctgaagc ggccagaata gccgcttctt aatttgccgg atgttccggc aaacgaaaaa
    27901 ttacttcttc ttcgctttcg ggttcggcag gtcggtaatg ctaccttcga acacttctgc
    27961 cgccaggccc acagactcgt gcagagtcgg gtgcgcatgg atggtcagtg cgatgtcttc
    28021 agcgtcacaa cccatttcga ttgccaggcc gatttcaccc agcagctcac cgccgttagt
    28081 accgacaatt gcaccaccga tcacacggtg agattctttg tcgaaaatca gcttggtcat
    28141 accgtctgcg cagtcggaag cgatagcacg accagaagca gcccacggga aggtggcggt
    28201 ttcatagctg atgcctttct ctttcgcttc tttctcagtc agacctaccc atgcaacttc
    28261 tggttcggta taggcgatgg acgggataac tttcggatcg aagtagtgtt tcttaccggc
    28321 gataacttca gcggcaacgt gaccttcgtg aacacctttg tgtgccagca tcggctgacc
    28381 gacgatatcg ccgatagcaa agatgtgcgg tacgttagtg cgcagctgtt tgtcaacgcg
    28441 gatgaaacca cggtcgtcca cttctacgcc agctttgcct gcgtcgaggt ttttaccgtt
    28501 cggcacacga ccaatcgcta ccagcacggc gtcgtaacgc tgcggttcag cgggtgcttt
    28561 tttgccttcc atcgtcacat aaataccgtc ttctttcgct tcaacggcgg taactttggt
    28621 ttccagcatc aggttgaatt tcttgctgat acgcttggtg aagactttaa cgatgtcttt
    28681 gtcagctgcc gggataacct ggtcgaacat ttcaaccacg tcaatctgtg aacccagcgc
    28741 gtggtatacg gtgcccattt ccagaccgat gataccgcca cccattacca gcaggcgttc
    28801 tggtacttct ttcagttcca gcgcgtcagt ggagtcccag atacgcggat cttcatgcgg
    28861 aataaacggc agttgaatcg ggcgagaacc cgctgcaatg atcgcgttgt cgaagttgat
    28921 cacggtttta ccgttctcac cttcaacttc cagggtgtta gccccggtaa atttacccag
    28981 accgttgacc actttgactt tgcggccttt cgccatacca gccagaccac cggtcagctg
    29041 attgatcact ttctctttcc aggtacgaat cttgtcgata tcggttttcg gttcgccaaa
    29101 gacgataccg tgttcagcca gcgctttggc ttcttcgata acttttgcta cgtgcagcag
    29161 tgctttagaa gggatacagc cgacgttcag gcaaacaccg ccaagagtgt tgtaacgttc
    29221 tacgattacg gtttccagac ctaaatcagc gcaacggaag gcagcggagt aacctgcggg
    29281 gcctgcccca agtaccacga cctgagtttt gatttcagta ctcatcatga cctctatata
    29341 tttatctccg gcgggtctga cgtttttttt atgacgccca ttatccaccg ggtcgttcta
    29401 tccgtcggta ttttacaaaa ttgttaacaa tttttaaaca acaaacggca accgatttgt
    29461 ctattcgcta ataacctcaa tacattcatg agattaccag aaaaaagccg gccgttgggc
    29521 cggctctttt acttacatca ccagacggcg aatgtcagac agcgtgttgt taatgatggt
    29581 aatgaaacgg gcaccatcag caccgtcgat cacgcggtgg tcgaaggaga gagaaatcgg
    29641 cagcatcaga cgcggcacga actctttacc attccacacc ggctccagcg cggacttgga
    29701 aacgccgagg atagccactt ccggcgcgtt cacaatcggc gcgaagtggg tagtacccag
    29761 gccgccgatg ctggagatgg tgaagcaacc gccctgcatt tcgcccgcag tcagcttacc
    29821 gtcacgcgct ttcttagaaa tagtcatcag ctcgcgagac agctcgatga tgcctttctt
    29881 gttgacgtct ttgaataccg ggacaaccag accgttcggg gtatccaccg ccacaccgat
    29941 gttgatgtat ttcttcaggg tcagacgctg accgtcttcc gacagcgaac tgttgaagcg
    30001 aggcatctgc tcaagagctg cagcaacggc tttcatgatg aagacaaccg gggtgatctt
    30061 cacatccagc ttacgtttcg ccgcttcttc gttctgctgt ttacggaacg cttccagctc
    30121 ggtgatatcg gttttgtcga agtgagtaac atgcgggatc atcacccagt tacggctcag
    30181 gttcgcacca gagattttct ggatgcggcc cagttccact tcttcgattt caccaaactt
    30241 gctgaagtcc accttcggcc acggcagcat gcctgggata ccaccgccag ttgccgccgg
    30301 agctgcttct gcacgtttga tagcttcttt cacgtaagcc tgaacgtctt cgcgcaggat
    30361 acgaccttta cggccagtgc ccttcacttt cgccaggtta acgccaaact cgcgtgccag
    30421 acggcggatc agcggagtcg cgtgaacgta agcgtcattt tcagcaaatt cagatttgcc
    30481 ttcggctttt gcagccggag ctgctgccgg ggcttcagct tttgctgccg gagccggcgc
    30541 tgccgcttcc tgtttcgcag gagctgccgc aggcgctgcg ccttcaactt cgaagatcat
    30601 aatcagcgaa ccagttttca ctttatcgcc aacgttgact ttcagttcct tcacgacgcc
    30661 tgcaaacgga gccgggactt ccatagaagc tttatcgcct tctacggtga tcagtgactg
    30721 ttcagcggca actttgtcgc ccactttcac catcacttcg gtcacttcaa cttcgtcacc
    30781 gccgatatcc ggaacgttaa cttctttcac gccagccgct ggtgcagggg ccgctgccgg
    30841 agccgcttcc tgtttagccg ccggagctgc cgcgccagct tcacccgcga cttcgaagac
    30901 cataatcagc gagccggtag acactttgtc acccacgttc actttgatct ctttcacggt
    30961 acctgcaaac ggagccggaa cttccataga agccttgtcg ccttctacgg tgatcagcga
    31021 ctgttcagct tcaactttat cgccaacttt caccaggatt tcggtcactt caacttcgtc
    31081 gctgccgata tccggaacgt taacgtcttt tgctgccgca gccgctggtg ctgctgccgg
    31141 agctgcttct ttcttctctt ctgcctgagc aggtgcagcg tctgctgcac cgtcggcgga
    31201 atcgaaaatc ataatcagtg cgccggtctg ggttttatcg ccaacagaga ctttgatctc
    31261 tttaacgata cccgcctgcg gagatggtac ttccatagag gctttgtcgc cttctacggt
    31321 gatcagcgac tgttcggctt caactttgtc gcccactttg accaggatct cggtgatttc
    31381 aacttcatca gccccgatgt ccggtacttt gatttcgata gccattattc ttttacctct
    31441 tacgccagac gcgggttaac tttatctgta tcgatgttga atttggcgat tgcgtcagca
    31501 accactttct tatcgatttc gccacgttta gccagttcgc ccagcgccgc aaccacgaca
    31561 taagaagcat caacttcaaa gtggtgacgc aggttctcac ggctgtcgga acgaccgaag
    31621 ccatcagtac ccagtacgcg gtagtcgtca gccggtacgt aagtacggac ctgctcagcg
    31681 aacagtttca tatagtcggt agatgccact gccggagcgt cgttcatcac ctgagcgata
    31741 tacggaacgc gcggagtttc cagcgggtgc agcatgttcc agcgttcaca atcctgacca
    31801 tcacgcgcca gttcggtgaa ggaagtcacg ctataaacgt cagaacctac gccgtaatct
    31861 ttcgccagga tctctgccgc ttcacggacg tgacgcagga tagaaccgga gcccagcagc
    31921 tgaactttac ctttgctacc ttcaatggtt tcgagtttgt agataccttt acggatacct
    31981 tcctcagcac cttccggcat tgccggcata tggtagtttt cgttcagcgt agtgatgtag
    32041 tagtaaacgt tctcttgttt ttcaccgtac atacgctcca gaccgtcatg catgatgaca
    32101 gcaacttcgt aagcgtaagc cgggtcgtaa gagatacagt tcgggatagt cagcgactga
    32161 atatggctgt gaccatcttc gtgctgcaga ccttcgccgt tcagggtggt acgaccggaa
    32221 gtaccgccga tcaggaagcc acgcgcttgc tggtcgccag ccgcccagca cagatcgccg
    32281 atacgctgga aaccgaacat cgagtaatag atgtagaacg ggatcatcgg cagattgttg
    32341 gtgctgtaag aggtcgccgc tgccagccag gaacaacctg cgcccagctc gttgatccct
    32401 tcctgcagaa tctgaccttt ctcgtcttct ttatagtaag caacctgctc gcggtcctgc
    32461 ggggtgtact gctgaccatt cgggctgtaa ataccaatct gacggaacag accttccata
    32521 ccgaaagtac gcgcttcgtc ggcgatgatc ggtaccagac gatctttgat cgacttgttc
    32581 ttcagcatca cgttcagagc acgaacgaaa gcgatagtgg tagagatctc tttgctctgc
    32641 tcttccagca gcgcgccgaa atcttgcagg ctcggcagct ccagcttctc ggtgaagttc
    32701 ggctggcggc ttggcagata accgtgcagt ttctgacgtt gagcgtgcag ataggtatgc
    32761 tcttcagaac cttccgggaa ggtgatgtac ggcagttttt cgatatcagc atcagatacc
    32821 ggcacattga aacggtcgcg gatatgacgc acaccgtcca tgttcatttt cttaacctgg
    32881 tgcgcaatgt ttttaccttc agccgcgtcg cccatgccat aacctttaat ggtatgagca
    32941 aggattactg tcgctttgcc tttggtttcc tgcgctttct tgaatgcagc gtagattttc
    33001 ttcggatcgt gaccaccacg gttcagtgcc cagatctgct cgtcagtcca gtctgcaacc
    33061 agtgctgcgg tttcaggata tttaccgaag aagtgttcgc gaacatacgc accatctttc
    33121 gatttgaagg tctggtaatc gccgtcaacg gtttcgttca tcagctggat cagtttaccg
    33181 ctggtatctt tacgcagcag ttcatcccaa cggctacccc acatcacttt aatcacgttc
    33241 cagccagcac cttcgaagat gccttccagt tcgttgatga tcttgccgtt accggtgacc
    33301 gggccgtcaa gacgctgcag gttacagttg ataacgaaga ccagattatc cagtttttca
    33361 cgggtagcga tggtgatcgc acctttggat tccggttcgt ccatttcacc gtcgccgagg
    33421 aacgcgtaaa cggtttgttt agaggtatct ttcaggccac ggtgttccag atatttcagg
    33481 aatttagcct ggtaaatagc accaatcgga cccagcccca tagatacggt cgggaactgc
    33541 cagaattccg gcatcagttt cgggtgcgga taggaagaga ggccattgcc gtgaacttcc
    33601 tgacggaagt tatccagctg ctcctgagtc agacgacctt ccaggaaagc acgagcgtaa
    33661 acgcctgggg agatgtggcc ctggaagtaa accaggtcgc cgccatcctg ctcgttgcgt
    33721 gcacggaaga agtggttaaa gcacacatca taaatggttg cggaagactg gaaggacgcc
    33781 atgtggccgc ccagttcgag gtcttttttc gacgcacgca gcaccgtcat gatggcgttc
    33841 cagcggatag ctgaacgaat acggcgttcc agttccagat tacccggata ctccggttgt
    33901 tcttcaacgg ggatggtgtt gatgtagttg ctgataccgg tgcctgcagc tacgttgact
    33961 ccgcctttgc gggcttcagc aagcagttgg tcgatcagat actgagcacg ctcaacacct
    34021 tcttcacgga tgaccgattc gatcgcctgg agccagtcgc gagtttcgat cggatccacg
    34081 tcatttggga aacgttctga catgggttat tccttatcta tctaataacg ttgagttttc
    34141 tggaacctgt cccattgaac tctcgccgga aagctcaata agacaggttc tacgtttagt
    34201 tgccgcgctt ttatatgcgc ttgatttaca acatcttctg gataattttt accagaaaaa
    34261 tcactaattc tttcgttgct ccagacgacg cagagaacgc tcacggcggc tctcttcacg
    34321 gcttctgtcg agcaaaattt cttcgataaa ggccagatgg cgatgcgatg cttcgcgcgc
    34381 ttcttccggc ttaccggcca taatcgcttc aaatatgcgg gtgcggtgac tactcaccag
    34441 cggcagcatc tcgcgacgcg aatagagcaa ttcgaagttc tggcggacat tctgagccaa
    34501 catcggctcc atacacctta gcagatgaag cagaaccaca ttgtgggccg cttcggtgac
    34561 ggcaatctga tactggagta cggcatttga ttccgcgtcc agatcgccag actgctgcgc
    34621 cagctctatg gcgtggtgga gttcacggat gcgttccttg tcttcatcgg tactacgcag
    34681 cgcggcgtaa taagcggcga taccttccag ggcgtgtcgt gtttcgagca agtcatactg
    34741 tgattcagga tggtcggaga gcagctccac cagcggatcg ctgaagcttt gccataggct
    34801 gctctggaca aaagtgccgc caccctggcg acgaagcaac aagcccttcg cttcgagacg
    34861 ttgaatcgcc tcacgcaagg agggacggga gacgtcgaac tgttttgcca gttcacgttc
    34921 cggtgggagt ttttcgcccg ggcggagagt gccttcgagg atcaaaaact ccagttgctg
    34981 ctcaatcaca tcggagagtt ttggttggcg gattttgctg taggccatga gttcctgtct
    35041 taagccactt gccgaagtca attggtctta ccaatttcat gtctgtgacg ctaaagtaac
    35101 aaagtattca ccttatgtcc atacaggttt tgattgaaat catgaaactg tgcacatttt
    35161 aacaacttga catatataac gtttcaaagt tgtaactatg cacaaatgtt aaatttaccc
    35221 atcaaggggt gaccttaaca gaattgaaac gaaaaaatat ttcctctgaa ccgattcacc
    35281 aagcaacaaa acgcataaca attcaattat gtgaattaat atgtaaaatg aatgttcagg
    35341 gaaggagaga aaggtagtat ttacctatgt tttcttttta cattgcttat tatcactgca
    35401 atcgccaccg taaccttctc gtagcgaaaa tcatcactta accagaacgt gatgattttc
    35461 aggtgacaga ttctgtttag agttcattct gaatctttga acaaaggcaa tccttgcgaa
    35521 cgccgccatt ctttgacgat tttcaggatg ttttcaggct tatcatctcc cttctttgca
    35581 ggatagtaga taagatcgct catcaacgga tgttgtgtaa ttctggtaag atgttcttct
    35641 agatcatccc aatatttgtc aagctcttcc ccttttaatt gagcgttatc cccggttgga
    35701 tttgtaaact cgccaagcaa ctcaatgaat tcccgctctg tgtaatcttc tattttatct
    35761 ttaaaattat acatttaact atctcctgta ataaatttca taatgcaaat gaggtgtgcc
    35821 ctccttaaat tatcaatatc gtaaatttcg ccactatatt caatcgcaac acaatgatac
    35881 atgtggaatt ttttaacgat ttctttaggg ccataatgcc aaccttcaag aggtgttttt
    35941 gcgattaatc cttgttttat acgtgtttga ttaccacttg aaaactgcgc tatcaactcc
    36001 ggatactgac tcacctccag ccaaagggta tcggtgaaag cgggaaattg ataaaataac
    36061 ctaatgagat ttcagtattt tacctgtttt ttaagagcac ccacattatt attttatgtt
    36121 attaacatca tcgcattagc cagacgcgat gatgttccat atatagtttt attatctact
    36181 ttgtttagag tctttaaaca agggtagccc ctgtgaacgc cgccattctt tgacaatttt
    36241 caggatgttt tcaggcttat catctccttg acttttaggg tagaaaatta gatcgctccc
    36301 tgacggatgc tgtgtaattt caataaaatg atctactaat gaatcccagt acatttctag
    36361 ctccttacct tttaagggct ttccatcttt catagttgat ctcaatccgc ctaaaaaatt
    36421 aagaaattcc tcctcggtat aatcttcgag cttttcttta aacatattta tctcctgtag
    36481 tgaatctcat cgtgtagtct gggggtaaca attcgtaaat tatcaatatc gtatacgctg
    36541 ccgccatatt caattgcaac acgatgatgt atctggaatt ttttaacgat ttctttaggg
    36601 ccataatgcc aaccttcaa
//
LOCUS       DADSYM010000026        36070 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2415
            SAMN08039579-rid11723173.denovo.026, whole genome shotgun sequence.
ACCESSION   DADSYM010000026 DADSYM010000000
VERSION     DADSYM010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039579
            Sequence Read Archive: SRR6362533
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 36070)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36070)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980162.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 03:39:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,484
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 4,347
            CDSs (with protein)               :: 4,347
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 43 of 85
            Pseudo Genes (incomplete)         :: 37 of 85
            Pseudo Genes (internal stop)      :: 19 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36070
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039579-rid11723173.denovo.026"
                     /strain="LGE2415"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8302 N 95.9628 W"
                     /collection_date="2015-08-24"
     gene            129..1499
                     /gene="aroP"
                     /locus_tag="J1Y83_001102"
     CDS             129..1499
                     /gene="aroP"
                     /locus_tag="J1Y83_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transporter AroP"
                     /protein_id="HBA7629270.1"
                     /translation="MEGQQHGEQLKRGLKNRHIQLIALGGAIGTGLFLGSASVIQSAG
                     PGIILGYAIAGFIAFLIMRQLGEMVVEEPVAGSFSHFAYKYWGSFAGFASGWNYWVLY
                     VLVAMAELTAVGKYIQFWYPEIPTWVSAAVFFVVINAINLTNVKVFGEMEFWFAIIKV
                     IAVVAMIIFGAWLLFSGNGGPQASVSNLWDQGGFLPHGFTGLVMMMAIIMFSFGGLEL
                     VGITAAEADNPEQSIPKATNQVIYRILIFYIGSLAVLLSLMPWTRVTADTSPFVLIFH
                     ELGDTFVANALNIVVLTAALSVYNSCVYCNSRMLFGLAQQGNAPKALASVDKRGVPVN
                     TILVSALVTALCVLINYLAPESAFGLLMALVVSALVINWAMISLAHMKFRRAKQEQGV
                     VTRFPALLYPLGNWICLLFMAAVLVIMLMTPGMAISVYLIPVWLIVLGIGYLFKEKTA
                     KAVKAH"
     gene            complement(1542..2396)
                     /gene="ampE"
                     /locus_tag="J1Y83_001103"
     CDS             complement(1542..2396)
                     /gene="ampE"
                     /locus_tag="J1Y83_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase regulator AmpE"
                     /protein_id="HBA7629271.1"
                     /translation="MTLFTTLLVLIFERLFKLGEHWQLDHRLEAFFRRVKHFSLGRTL
                     GMTIIAMGVTFLLLRALQGVLFNVPTLLVWLLIGLLCIGAGKVRLHYHAYLTAASRND
                     SHARATMAGELTMIHGVPAGCDEREYLRELQNALLWINFRFYLAPLFWLIVGGTWGPV
                     TLMGYAFLRAWQYWLARYQTPHHRLQSGIDGVLHVLDWVPVRLAGVVYALIGHGEKAL
                     PAWFASLGDFHTSQYQVLTRLAQFSLAREPHVDKVETPKAAVSMAKKTSFVVVVVIAL
                     LTIYGALV"
     gene            complement(2393..2944)
                     /gene="ampD"
                     /locus_tag="J1Y83_001104"
     CDS             complement(2393..2944)
                     /gene="ampD"
                     /locus_tag="J1Y83_001104"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,6-anhydro-N-acetylmuramyl-L-alanine amidase
                     AmpD"
                     /protein_id="HBA7629272.1"
                     /translation="MLLEQGWLVGARRVPSPHYDCRPDDETPTLLVVHNISLPPGEFG
                     GPWIDALFTGTIDPQAHPFFAEIAHLRVSAHCLIRRDGEIVQYVPFDKRAWHAGVSQY
                     QGRERCNDFSIGIELEGTDTLAYTDAQYQQLAAVTRALIDRYPDIANNMTGHCDIAPD
                     RKTDPGPAFDWARFRALVSKETT"
     gene            3032..3925
                     /gene="nadC"
                     /locus_tag="J1Y83_001105"
     CDS             3032..3925
                     /gene="nadC"
                     /locus_tag="J1Y83_001105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HBA7629273.1"
                     /translation="MPPRRYNPDTRRDELLERINLDIPGAVAQALREDLGGTVDANND
                     ITAKLLPENSRSHATVITRENGVFCGKRWVEEVFIQLAGDDVTIIWHVDDGDVINANQ
                     PLFELEGPSRVLLTGERTALNFVQTLSGVASKVRHYVELLEGTNTQLLDTRKTLPGLR
                     SALKYAVLCGGGANHRLGLSDAFLIKENHIIASGSVRQAVEKASWLHPDAPVEVEVEN
                     LEELDEALKAGADIIMLDNFETEQMREAVKRTNGKALLEVSGNVTDKTLREFAETGVD
                     FISVGALTKHVQALDLSMRFR"
     gene            4129..4569
                     /gene="ppdD"
                     /locus_tag="J1Y83_001106"
     CDS             4129..4569
                     /gene="ppdD"
                     /locus_tag="J1Y83_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent pilin"
                     /protein_id="HBA7629274.1"
                     /translation="MDKQRGFTLIELMVVIGIIAILSAIGIPAYQNYLRKAALTDMLQ
                     TFVPYRTAVELCALEHGGLNTCDGGSNGIPSPTTTRYVSAMSVVKGVVSLTGQESLNG
                     LSIVMTPGWDNANGVTGWTRNCNIQSDSALQQACEDVFRFDDAN"
     gene            4579..5964
                     /gene="gspE"
                     /locus_tag="J1Y83_001107"
     CDS             4579..5964
                     /gene="gspE"
                     /locus_tag="J1Y83_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspE"
                     /protein_id="HBA7629275.1"
                     /translation="MNIPQLTALCLRYQGVLLDASEEVVHVAVVDAPSHELLDALHFA
                     TTKRIEITCWTRQQMEGHASRTQQTLPVAVQEKHQPKAELLTRTLQSALEQRASDIHI
                     EPADNAYRIRLRIDGVLHPLPDVSPDAGVALTARLKVLGNLDIAEHRLPQDGQFTVEL
                     AGNAVSFRIATLACRGGEKVVLRLLQQVNQALDVNTLGMQPSQLVDFAHALQQPQGLV
                     LVTGPTGSGKTVTLYSALQMLNTADINICSVEDPVEIPIAGLNQTQIHSRAGLTFQGV
                     LRALLRQDPDVIMIGEIRDGETAEIAIKAAQTGHLVLSTLHTNSTCETLVRLQQMGVA
                     RWMLSSALTLVIAQRLVRKLCPHCRRQQGEPIHIPDNVWPSPLPHWQAPGCVHCYHGF
                     YGRTALFEVLPITPVIRQLISANTDVESLETHARQAGMRTLFENGCLAVEQGLTTFEE
                     LIRVLGMPHGE"
     gene            5954..7156
                     /gene="hofC"
                     /locus_tag="J1Y83_001108"
     CDS             5954..7156
                     /gene="hofC"
                     /locus_tag="J1Y83_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein transport protein HofC"
                     /protein_id="HBA7629276.1"
                     /translation="MASKQLWRWHGITGDGNAQDGMLWAESRALLLMALQQQMVTPLS
                     LKRITINSAQWRGDKSAEVIHQLATLLKAGLTLSEGLALLAEQHPSKQWQALLQSLAH
                     DLEQGVAFSNALLPWSEVFPPLYQAMIRTGELTGKLDECCFELARQQKAQRQLTDKVK
                     SALRYPIIILAMAIMVVVAMLHFVLPEFAAIYKTFNTPLPALTQGIMTLASFSGEWGW
                     LLVLFGFLLAIANKLLMRHPTRLIARQKLLLRIPIMGSLMRGQKLTQIFTILTLTQSA
                     GISFLQGVESVRETMRCPYWVQLLTQIQHDISNGHPIWLALKNAGEFSPLCLQLVRTG
                     EASGSLDLMLDNLAHHHRDNTMALADNLAALLEPALLIITGGIIGTLVVAMYLPIFHL
                     GDAMSGMG"
     gene            complement(7191..8234)
                     /gene="guaC"
                     /locus_tag="J1Y83_001109"
     CDS             complement(7191..8234)
                     /gene="guaC"
                     /locus_tag="J1Y83_001109"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008461816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HBA7629277.1"
                     /translation="MRIEEDLKLGFKDVLIRPKRSTLKSRSDVELERQFTFKHSGQSW
                     SGVPIIAANMDTVGTFSMASALASFDILTAVHKHYSVEEWQAFINNSSADVLKHVMVS
                     TGTSDADFEKTKQILDLNPALNFVCIDVANGYSEHFVQFVAKAREAWPTKTICAGNVV
                     TGEMCEELILSGADIVKVGIGPGSVCTTRVKTGVGYPQLSAVIECADAAHGLGGMIVS
                     DGGCTTPGDVAKAFGGGADFVMLGGMLAGHEESGGRIVEENGEKFMLFYGMSSESAMK
                     RHVGGVAEYRAAEGKTVKLPLRGPVENTARDILGGLRSACTYVGASRLKELTKRTTFI
                     RVQEQENRIFNNL"
     gene            8459..9079
                     /gene="coaE"
                     /locus_tag="J1Y83_001110"
     CDS             8459..9079
                     /gene="coaE"
                     /locus_tag="J1Y83_001110"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HBA7629278.1"
                     /translation="MRYIVALTGGIGSGKSTVANAFADLGINVIDADIIARQVVEPGA
                     PALHAIADHFGANMIAADGTLQRRALRERIFANPEEKNWLNALLHPLIQQETQHQIQR
                     ATSPYVLWVVPLLVENSLYKKANRVLVVDVSPETQLKRTMQRDDVTREHVEQILAAQA
                     TREARLAVADDVIDNNGAPDAIASDVARLHAHYLQLASQFVSQEKP"
     gene            9079..9822
                     /gene="zapD"
                     /locus_tag="J1Y83_001111"
     CDS             9079..9822
                     /gene="zapD"
                     /locus_tag="J1Y83_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapD"
                     /protein_id="HBA7629279.1"
                     /translation="MQTQVLFEHPLNEKMRTWLRIEFLIQQLTVNLPIVDHAGALHFF
                     RNVSELLDVFERGEVRTELLKELDRQQRKLQTWIGVPGVDQSRIEALIQQLKAAGSVL
                     ISAPRIGQFLREDRLIALVRQRLSIPGGCCSFDLPTLHIWLHLPQAQRDSQVETWIAS
                     LNPLTQALTMVLDLIRQSAPFRKQTSLNGFYQDNGGDADLLRLNLSLDSQLYPQISGH
                     KSRFAIRFMPLDSENGQVPERLDFELACC"
     gene            9832..10029
                     /gene="yacG"
                     /locus_tag="J1Y83_001112"
     CDS             9832..10029
                     /gene="yacG"
                     /locus_tag="J1Y83_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor YacG"
                     /protein_id="HBA7629280.1"
                     /translation="MSETITVNCPTCGKTVVWGEISPFRPFCSKRCQLIDLGEWAAEE
                     KRIPSSGDLSESDDWSEEPKQ"
     gene            complement(10117..10515)
                     /gene="mutT"
                     /locus_tag="J1Y83_001113"
     CDS             complement(10117..10515)
                     /gene="mutT"
                     /locus_tag="J1Y83_001113"
                     /EC_number="3.6.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase MutT"
                     /protein_id="HBA7629281.1"
                     /translation="MKKLQIAVGIIRNENNEIFITRRAADAHMANKLEFPGGKVEMGE
                     TPEQAVVRELQEEVGITPQHFSLFEKLEYEFPDRHITLWFWLVESWEGEPWGKEGQPG
                     EWMSLVGLNADDFPPANEPVIAKLKRVYAG"
     gene            complement(10575..13280)
                     /gene="secA"
                     /locus_tag="J1Y83_001114"
     CDS             complement(10575..13280)
                     /gene="secA"
                     /locus_tag="J1Y83_001114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HBA7629282.1"
                     /translation="MLIKLLTKVFGSRNDRTLRRMRKVVNIINAMEPEMEKLSDEELK
                     GKTAEFRARLEKGEVLENLIPEAFAVVREASKRVFGMRHFDVQLLGGMVLNERCIAEM
                     RTGEGKTLTATLPAYLNALTGKGVHVVTVNDYLAQRDAENNRPLFEFLGLTVGINLPG
                     MPAPAKREAYAADITYGTNNEYGFDYLRDNMAFSPEERVQRKLHYALVDEVDSILIDE
                     ARTPLIISGPAEDSSEMYKRVNKIIPHLIRQEKEDSETFQGEGHFSVDEKSRQVNLTE
                     RGLVLIEELLVKEGIMDEGESLYSPANIMLMHHVTAALRAHALFTRDVDYIVKDGEVI
                     IVDEHTGRTMQGRRWSDGLHQAVEAKEGVQIQNENQTLASITFQNYFRLYEKLAGMTG
                     TADTEAFEFSSIYKLDTVVVPTNRPMIRKDLPDLVYMTEAEKIQAIIEDIKERTAKGQ
                     PVLVGTISIEKSELVSNELTKAGIKHNVLNAKFHANEAAIVAQAGYPAAVTIATNMAG
                     RGTDIVLGGSWQAEVAALENPTAEQIEKIKADWQVRHDAVLAAGGLHIIGTERHESRR
                     IDNQLRGRSGRQGDAGSSRFYLSMEDALMRIFASDRVSGMMRKLGMKPGEAIEHPWVT
                     KAIANAQRKVESRNFDIRKQLLEYDDVANDQRRAIYSQRNELLDVSDVSETINSIRED
                     VFKATIDAYIPPQSLEEMWDIPGLQERLKNDFDLDLPIAEWLDKEPELHEETLRERIL
                     AQSIEVYQRKEEVVGAEMMRHFEKGVMLQTLDSLWKEHLAAMDYLRQGIHLRGYAQKD
                     PKQEYKRESFSMFAAMLESLKYEVISTLSKVQVRMPEEVEELEQQRRMEAERLAQMQQ
                     LSHQDDDSAAAAALAAQTGERKVGRNDPCPCGSGKKYKQCHGRLQ"
     gene            complement(13342..13854)
                     /gene="secM"
                     /locus_tag="J1Y83_001115"
     CDS             complement(13342..13854)
                     /gene="secM"
                     /locus_tag="J1Y83_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308128.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secA regulator SecM"
                     /protein_id="HBA7629283.1"
                     /translation="MSGILTRWRQFGKRYFWPHLLLGMVAASLGLPALSNAAEPNAPA
                     KATTRNHEPSAKVNFGQLALLEANTRRPNSNYSVDYWHQHAIRTVIRHLSFAMAPQTL
                     PVAEESLPLQAQHLALLDTLSALLTQEGTPSEKGYRIDYAHFTPQAKFSTPVWISQAQ
                     GIRAGPQRLS"
     gene            complement(14086..15003)
                     /gene="lpxC"
                     /locus_tag="J1Y83_001116"
     CDS             complement(14086..15003)
                     /gene="lpxC"
                     /locus_tag="J1Y83_001116"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000595491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HBA7629284.1"
                     /translation="MIKQRTLKRIVQATGVGLHTGKKVTLTLRPAPANTGVIYRRTDL
                     NPPVDFPADAKSVRDTMLCTCLVNEHDVRISTVEHLNAALAGLGIDNIVIEVNAPEIP
                     IMDGSAAPFVYLLLDAGIDELNCAKKFVRIKETVRVEDGDKWAEFKPYNGFSLDFTID
                     FNHPAIDSSNQRYAMNFSADAFMRQISRARTFGFMRDIEYLQSRGLCLGGSFDCAIVV
                     DDYRVLNEDGLRFEDEFVRHKMLDAIGDLFMCGHNIIGAFTAYKSGHALNNKLLQAVL
                     AKQEAWEYVTFQDDAELPLAFKAPSAVLA"
     gene            complement(15104..16255)
                     /gene="ftsZ"
                     /locus_tag="J1Y83_001117"
     CDS             complement(15104..16255)
                     /gene="ftsZ"
                     /locus_tag="J1Y83_001117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015965916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HBA7629285.1"
                     /translation="MFEPMELTNDAVIKVIGVGGGGGNAVEHMVRERIEGVEFFAVNT
                     DAQALRKTAVGQTIQIGSGITKGLGAGANPEVGRNAADEDRDALRAALEGADMVFIAA
                     GMGGGTGTGAAPVVAEVAKDLGILTVAVVTKPFNFEGKKRMAFAEQGITELSKHVDSL
                     ITIPNDKLLKVLGRGISLLDAFGAANDVLKGAVQGIAELITRPGLMNVDFADVRTVMS
                     EMGYAMMGSGVASGEDRAEEAAEMAISSPLLEDIDLSGARGVLVNITAGFDLRLDEFE
                     TVGNTIRAFASDNATVVIGTSLDPDMNDELRVTVVATGIGMDKRPEITLVTNKQVQQP
                     VMDRYQQHGMAPLTQEQKPVAKVVNDNAPQTAKEPDYLDIPAFLRKQAD"
     gene            complement(16316..17578)
                     /gene="ftsA"
                     /locus_tag="J1Y83_001118"
     CDS             complement(16316..17578)
                     /gene="ftsA"
                     /locus_tag="J1Y83_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017374018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HBA7629286.1"
                     /translation="MIKATDRKLVVGLEIGTAKVAALVGEVLPDGMVNIIGVGSCPSR
                     GMDKGGVNDLESVVKCVQRAIDQAELMADCQISSVYLALSGKHISCQNEIGMVPISEE
                     EVTQEDVENVVHTAKSVRVRDEHRVLHVIPQEYAIDYQEGIKNPVGLSGVRMQAKVHL
                     ITCHNDMAKNIVKAVERCGLKVDQLIFAGLASSYSVLTEDERELGVCVVDIGGGTMDI
                     AVYTGGALRHTKVIPYAGNVVTSDIAYAFGTPPSDAEAIKVRHGCALGSIVGKDESVE
                     VPSVGGRPPRSLQRQTLAEVIEPRYTELLNLVNEEILQLQEKLRQQGVKHHLAAGIVL
                     TGGAAQIEGLAACAQRVFHTQVRIGAPLNITGLTDYAQEPYYSTAVGLLHYGKESHLN
                     GEAEVEKRVTASVGSWIKRLNSWLRKEF"
     gene            complement(17575..18405)
                     /gene="ftsQ"
                     /locus_tag="J1Y83_001119"
     CDS             complement(17575..18405)
                     /gene="ftsQ"
                     /locus_tag="J1Y83_001119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ"
                     /protein_id="HBA7629287.1"
                     /translation="MSQAALNTRNSEEEVSSRRNNGTRLAGILFLLTVLTTVLVSGWV
                     VLGWMEDAQRLPLSKLVLTGERHYTRNDDIRQSILALGEPGTFMTQDVNIIQTQIEQR
                     LPWIKQVSVRKQWPDELKIHLVEYVPIARWNDQHMVDAEGNTFSVPPDRTSKQVLPML
                     YGPEGSANEVLQGYREMGQMLAKDRFTLKEAAMTARRSWQLTLNNDIKLNLGRGDTMK
                     RLARFVELYPVLQQQAQTDGKRISYVDLRYDSGAAVGWAPLPPGESTQQQNQAQAEQQ
                     "
     gene            complement(18407..19327)
                     /locus_tag="J1Y83_001120"
     CDS             complement(18407..19327)
                     /locus_tag="J1Y83_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HBA7629288.1"
                     /translation="MTDKIAVLLGGTSAEREVSLNSGAAVLAGLREGGIDAYPVDPKE
                     VDVTQLKSMGFQKVFIALHGRGGEDGTLQGMLELMGLPYTGSGVMASALSMDKLRSKL
                     LWQGAGLPVAPWVALTRAEFEKGLNDKQLAEISSLGLPVIVKPSREGSSVGMSKVVAE
                     NALQDALRLAFQHDEEVLIEKWLSGPEFTVAILGEEILPSIRIQPAGTFYDYEAKYLS
                     DETQYFCPAGLEASQEANLQALVLKAWTTLGCKGWGRIDVMLDSDGQFYLLEANTSPG
                     MTSHSLVPMAARQAGMSFSQLVVRILELAD"
     gene            complement(19320..20795)
                     /gene="murC"
                     /locus_tag="J1Y83_001121"
     CDS             complement(19320..20795)
                     /gene="murC"
                     /locus_tag="J1Y83_001121"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HBA7629289.1"
                     /translation="MNTQQLAKLRSIVPEMRRVRHIHFVGIGGAGMGGIAEVLANEGY
                     QISGSDLAPNPVTQQLMNLGATIYFNHRPENVRDASVVVVSSAISADNPEIVAAHEAR
                     IPVIRRAEMLAELMRFRHGIAIAGTHGKTTTTAMVSSIYAEAGLDPTFVNGGLVKAAG
                     VHARLGHGRYLIAEADESDASFLHLQPMVAIVTNIEADHMDTYQGDFENLKQTFINFL
                     HNLPFYGRAVMCVDDPVIRELLPRVGRQTTTYGFSEDADVRVEDYQQIGPQGHFTLLR
                     QDKEPMRVTLNAPGRHNALNAAAAVAVATEEGIDDEAILRALESFQGTGRRFDFLGEF
                     PLEPVNGKSGTAMLVDDYGHHPTEVDATIKAARAGWPDKNLVMLFQPHRFTRTRDLYD
                     DFANVLTQVDTLLMLEVYPAGEAPIPGADSRSLCRTIRGRGKIDPILVPDPAQVAEML
                     APVLTGNDLILVQGAGNIGKIARSLAEIKLKPQTPEEEQHD"
     gene            complement(20849..21916)
                     /gene="murG"
                     /locus_tag="J1Y83_001122"
     CDS             complement(20849..21916)
                     /gene="murG"
                     /locus_tag="J1Y83_001122"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HBA7629290.1"
                     /translation="MSGQGKRLMVMAGGTGGHVFPGLAVAHHLMAQGWQVRWLGTADR
                     MEADLVPKHGIEIDFIRISGLRGKGIKALIAAPLRIFNAWRQARAIMKAYKPDVVLGM
                     GGYVSGPGGLAAWSLGIPVVLHEQNGIAGLTNKWLAKIATKVMQAFPGAFPNAEVVGN
                     PVRTDVLALPLPQQRLAGREGPVRVLVVGGSQGARILNQTMPQVAAKLGDSVTIWHQS
                     GKGSQQSVEQAYAEAGQPQHKVTEFIDDMAAAYAWADVVVCRSGALTVSEIAAAGLPA
                     LFVPFQHKDRQQYWNALPLEKAGAAKIIEQSQLSVDAVANTLAGWSRETLLTMAERAR
                     AASIPDATERVANEVSRAARA"
     gene            complement(21913..23157)
                     /gene="ftsW"
                     /locus_tag="J1Y83_001123"
     CDS             complement(21913..23157)
                     /gene="ftsW"
                     /locus_tag="J1Y83_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsW"
                     /protein_id="HBA7629291.1"
                     /translation="MRLSLPRLKMPRLPGFSILVWISTALKGWVMGSREKDTDSLIMY
                     DRTLLWLTFGLAAIGFIMVTSASMPIGQRLTNDPFFFAKRDGVYLILAFILAIITLRL
                     PMEFWQRYSATMLLGSIILLMIVLVVGSSVKGASRWIDLGLLRIQPAELTKLSLFCYI
                     ANYLVRKGDEVRNNLRGFLKPMGVILVLAVLLLAQPDLGTVVVLFVTTLAMLFLAGAK
                     LWQFIAIIGMGISAVVLLILAEPYRIRRVTAFWNPWEDPFGSGYQLTQSLMAFGRGEL
                     WGQGLGNSVQKLEYLPEAHTDFIFAIIGEELGYVGVVLALLMVFFVAFRAMSIGRKAL
                     EIDHRFSGFLACSIGIWFSFQALVNVGAAAGMLPTKGLTLPLISYGGSSLLIMSTAIM
                     MLLRIDYETRLEKAQAFVRGSR"
     gene            complement(23157..24473)
                     /gene="murD"
                     /locus_tag="J1Y83_001124"
     CDS             complement(23157..24473)
                     /gene="murD"
                     /locus_tag="J1Y83_001124"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HBA7629292.1"
                     /translation="MADYQGKNVVIIGLGLTGLSCVDFFLARGVTPRVMDTRMTPPGL
                     DKLPEAVERHTGGLNDEWLMAADLIVASPGIALAHPSLSAAADAGIEIVGDIELFCRE
                     AQAPIVAITGSNGKSTVTTLVGEMAKAAGVNVGVGGNIGLPALMLLDDECELYVLELS
                     SFQLETTSSLQAVAATILNVTEDHMDRYPFGLQQYRAAKLRIYENAKVCVVNADDALT
                     MPIRGADERCVSFGVNMGDYHLNHQQGETWLRVKGEKVLNVKEMKLSGQHNYTNALAA
                     LALADAAGLPRASSLKALTTFTGLPHRFEVVLEHNGVRWVNDSKATNVGSTEAALNGL
                     HVDGTLHLLLGGDGKSADFSPLARYLNGDNVRLYCFGRDGAQLAALRPEVAEQTETME
                     QAMRLLATRVQPGDMVLLSPACASLDQFKNFEQRGNEFARLAKELG"
     gene            complement(24476..25558)
                     /gene="mraY"
                     /locus_tag="J1Y83_001125"
     CDS             complement(24476..25558)
                     /gene="mraY"
                     /locus_tag="J1Y83_001125"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HBA7629293.1"
                     /translation="MLVWLAEHLVKYYSGFNVFSYLTFRAIVSLLTALFISLWMGPRM
                     IAHLQKLSFGQVVRNDGPESHFSKRGTPTMGGIMILTAIVISVLLWAYPSNPYVWCVL
                     VVLVGYGVIGFVDDYRKVVRKDTKGLIARWKYFWMSVIALGVAFALYLAGKDTPATQL
                     VVPFFKDVMPQLGLFYILLAYFVIVGTGNAVNLTDGLDGLAIMPTVFVAGGFALVAWA
                     TGNMNFASYLHIPYLRHAGELVIVCTAIVGAGLGFLWFNTYPAQVFMGDVGSLALGGA
                     LGIIAVLLRQEFLLVIMGGVFVVETLSVILQVGSFKLRGQRIFRMAPIHHHYELKGWP
                     EPRVIVRFWIISLMLVLIGLATLKVR"
     gene            complement(25552..26910)
                     /gene="murF"
                     /locus_tag="J1Y83_001126"
     CDS             complement(25552..26910)
                     /gene="murF"
                     /locus_tag="J1Y83_001126"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HBA7629294.1"
                     /translation="MISVTLSQLTDILNGELQGADITLDAVTTDTRKLTPGCLFVALK
                     GERFDAHDFADQAKAGGAGALLVSRPLDIDLPQLIVKDTRLAFGELAAWVRQQVPARV
                     VALTGSSGKTSVKEMTAAILSQCGNTLYTAGNLNNDIGVPMTLLRLTPEYDYAVIELG
                     ANQQGEIAWTVSLTRPEAALVNNLAAAHLEGFGSLAGVAKAKGEIFSGLPENGIAIMN
                     ADNNDWLNWQSVIGSRKVWRFSPNAANSDFTATNIHVTSHGTEFTLQTPTGSVDVLLP
                     LPGRHNIANALAAAALSMSVGATLDAIKAGLANLKAVPGRLFPIKLAENQLLLDDSYN
                     ANVGSMTAAVQVLAEMPGYRVLVVGDMAELGAESEACHVQVGEAAKAAGIDCVLSVGK
                     QSHAISTASGVGEHFTDKTALIARLKSLIAEQQVITILVKGSRSAAMEEVVRALQENG
                     TC"
     gene            complement(26907..28394)
                     /gene="murE"
                     /locus_tag="J1Y83_001127"
     CDS             complement(26907..28394)
                     /gene="murE"
                     /locus_tag="J1Y83_001127"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HBA7629295.1"
                     /translation="MADRNLRDLLAPWVPDAPSRALREMTLDSRVAAAGDLFVAVVGH
                     QADGRRYIPQAIAQGVAAIIAEAKDEATDGEIREMHGVPVIYLSQLNERLSALAGRFY
                     HEPSDNLRLVGVTGTNGKTTTTQLLAQWSQLLGETSAVMGTVGNGLLGKVIPTENTTG
                     SAVDVQHELAGLVDQGATFCAMEVSSHGLVQHRVAALKFAASVFTNLSRDHLDYHGDM
                     EHYEAAKWLLYSAHHCGQAIINADDEVGRRWLAKLPDAVAVSMEDHINPNCHGRWLKA
                     TDVNYHDSGATIRFSSSWGDGEIESRLMGAFNVSNLLLALATLLALGYPLADLLKTAA
                     RLQPVCGRMEVFTAPGKPTVVVDYAHTPDALEKALQAARLHCAGKLWCVFGCGGDRDK
                     GKRPLMGAIAEEFADVAVVTDDNPRTEEPRAIINDILAGMLDAGYAKVMEGRAEAVTC
                     AVMQAKENDVVLVAGKGHEDYQIVGNQRLDYSDRVTVARLLGVIA"
     gene            complement(28381..30147)
                     /locus_tag="J1Y83_001128"
     CDS             complement(28381..30147)
                     /locus_tag="J1Y83_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000642204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase FtsI"
                     /protein_id="HBA7629296.1"
                     /translation="MKAAAKTQKPKRQEEHANFISWRFALLCGCILLALAFLLGRVAW
                     LQVISPDMLVKEGDMRSLRVQQVSTSRGMITDRSGRPLAVSVPVKAIWADPKEVHDAG
                     GISVGDRWKALANALNIPLDQLSARINANPKGRFIYLARQVNPDMADYIKKLKLPGIH
                     LREESRRYYPSGEVTAHLIGFTNVDSQGIEGVEKSFDKWLTGQPGERIVRKDRYGRVI
                     EDISSTDSQAAHNLTLSIDERLQALVYRELNNAVAFNKAESGSAVLVDVNTGEVLAMA
                     NSPSYNPNNLSGTPKEAMRNRTITDVFEPGSTVKPMVVMTALQRGVVRENSVLNTVPY
                     RINGHEIKDVARYSELTLTGVLQKSSNVGVSKLALAMPSSALVDTYSRFGLGKATNLG
                     LVGERSGLYPQKQRWSDIERATFSFGYGLMVTPLQLARVYATIGSYGIYRPLSITKVD
                     PPVPGERVFPESIVRTVVHMMESVALPGGGGVKAAIKGYRIAIKTGTAKKVGPDGRYI
                     NKYIAYTAGVAPASQPRFALVVVINDPQAGKYYGGAVSAPVFGAIMGGVLRTMNIEPD
                     ALTTGDKNEFVINQGEGTGGRS"
     gene            complement(30163..30528)
                     /gene="ftsL"
                     /locus_tag="J1Y83_001129"
     CDS             complement(30163..30528)
                     /gene="ftsL"
                     /locus_tag="J1Y83_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000625658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HBA7629297.1"
                     /translation="MISRVTEALSKVKGSMGSHERHALPGVIGDDLLRFGKLPLCLFI
                     CIILTAVTVVTTAHHTRLLTAQREQLVLERDALDIEWRNLILEENALGDHSRVERIAT
                     EKLQMQHVDPSQENIVVQK"
     gene            complement(30525..31466)
                     /gene="rsmH"
                     /locus_tag="J1Y83_001130"
     CDS             complement(30525..31466)
                     /gene="rsmH"
                     /locus_tag="J1Y83_001130"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HBA7629298.1"
                     /translation="MMENYKHTTVLLDEAVNGLNIRPDGTYIDGTFGRGGHSRLILSQ
                     LGEEGRLLAIDRDPQAIAVAKTIDDPRFSIIHGPFSALGEYVAERDLIGKIDGILLDL
                     GVSSPQLDDAERGFSFMRDGPLDMRMDPTRGQSAAEWLQTAEEADIAWVLKTYGEERF
                     AKRIARAIVERNREQPMTRTKELAEVVAAATPVKDKFKHPATRTFQAVRIWVNSELEE
                     IEQALKSSLNVLAPGGRLSIISFHSLEDRIVKRFMRENSRGPQVPAGLPMTEEQLKKL
                     GGRQLRALGKLMPGEEEVAENPRARSSVLRIAERTNA"
     gene            complement(31468..31926)
                     /gene="mraZ"
                     /locus_tag="J1Y83_001131"
     CDS             complement(31468..31926)
                     /gene="mraZ"
                     /locus_tag="J1Y83_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HBA7629299.1"
                     /translation="MFRGATLVNLDSKGRLSVPTRYREQLLENAAGQMVCTIDIHHPC
                     LLLYPLPEWEIIEQKLSRLSSMNPVERRVQRLLLGHASECQMDGAGRLLIAPVLRQHA
                     GLTKEVMLVGQFNKFELWDETTWHQQVKEDIDAEQLATGDLSERLQDLSL"
     gene            complement(32528..33532)
                     /gene="cra"
                     /locus_tag="J1Y83_001132"
     CDS             complement(32528..33532)
                     /gene="cra"
                     /locus_tag="J1Y83_001132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite repressor/activator"
                     /protein_id="HBA7629300.1"
                     /translation="MKLDEIARLAGVSRTTASYVINGKAKQYRVSDKTVEKVMAVVRE
                     HNYHPNAVAAGLRAGRTRSIGLVIPDLENTSYTRIANYLERQARQRGYQLLIACSEDQ
                     PDNEMRCIEHLLQRQVDAIIVSTSLPPEHPFYQRWANDPFPIVALDRALDREHFTSVV
                     GADQDDAEMLAEELRKFPAETVLYLGALPELSVSFLREQGFRTAWKDDPREVHFLYAN
                     SYEREAAAQLFEKWLETHPMPQALFTTSFALLQGVMDVTLRRDGKLPSDLAIATFGDN
                     ELLDFLQCPVLAVAQRHRDVAERVLEIVLASLDEPRKPKPGLTRIKRNLYRRGVLSRS
                     "
     gene            complement(33614..33700)
                     /locus_tag="J1Y83_001133"
     CDS             complement(33614..33700)
                     /locus_tag="J1Y83_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fruR/shl operon leader peptide"
                     /protein_id="HBA7629301.1"
                     /translation="MRNLQPNMSRWAFFAKSVGTWNKSSCRS"
     gene            complement(33712..34203)
                     /gene="ilvN"
                     /locus_tag="J1Y83_001134"
     CDS             complement(33712..34203)
                     /gene="ilvN"
                     /locus_tag="J1Y83_001134"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HBA7629302.1"
                     /translation="MRRILSVLLENESGALSRVIGLFSQRGYNIESLTVAPTDDPTLS
                     RMTIQTVGDEKVLEQIEKQLHKLVDVLRVSELGQGAHVEREIMLVKIQASGYGRDEVK
                     RNTEIFRGQIIDVTPSLYTVQLAGTSDKLDAFLASIRDVAKIVEVARSGVVGLSRGDK
                     IMR"
     gene            complement(34206..35930)
                     /gene="ilvI"
                     /locus_tag="J1Y83_001135"
     CDS             complement(34206..35930)
                     /gene="ilvI"
                     /locus_tag="J1Y83_001135"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308108.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 3 large subunit"
                     /protein_id="HBA7629303.1"
                     /translation="MEMLSGAEMVVRSLIDQGVKQVFGYPGGAVLDIYDALHTVGGID
                     HVLVRHEQAAVHMADGLARATGEVGVVLVTSGPGATNAITGIATAYMDSIPLVVLSGQ
                     VATSLIGYDAFQECDMVGISRPVVKHSFLVKQTEDIPQVLKKAFWLAASGRPGPVVVD
                     LPKDILNPANKLPYVWPESVSMRSYNPTTSGHKGQIKRALQTLVAAKKPVVYVGGGAI
                     TAGCHLQLKEAVEVLNLPVVSSLMGLGAFPATHRQALGMLGMHGTYEANMTMHNADVI
                     FAVGVRFDDRTTNNLAKYCPNATVLHIDIDPTSISKTVTADIPIVGDARQVLEQMLEL
                     LSQESAHQPLDEIRDWWQQIEQWRARQCLKYDTHSEKIKPQAVIETLWRLTNGDAYVT
                     SDVGQHQMFAALYYPFDKPRRWINSGGLGTMGFGLPAALGVKMALPEETVVCVTGDGS
                     IQMNIQELSTALQYELPVLVVNLNNRYLGMVKQWQDMIYSGRHSQSYMQSLPDFVRLA
                     EAYGHVGIQISHPQELESKLSEALEQVRNNRLVFVDVTVDGSEHVYPMQIRGGGMDEM
                     WLSKTERT"
BASE COUNT         8655 a        10111 c         8845 g         8459 t
ORIGIN      
        1 agttgaaagt actatcatca taattcagtt acataaaatg taaagaaacc aatacaacaa
       61 acggaattac aaaattacac acgcattgct gcgtagttca aaaaaacaac acaacacgag
      121 gtttcataat ggaaggtcaa cagcacggcg agcagctaaa gcgcggcctt aaaaaccgcc
      181 atattcagct tatcgcgctg ggtggcgcga tagggacagg gctattcctg ggtagcgcct
      241 ccgtaataca gtccgcaggg ccagggatta tcctgggtta cgccattgct ggttttatcg
      301 cctttctgat catgcgtcag ctaggtgaaa tggtggtcga agaacctgtc gcaggctcct
      361 ttagccactt tgcttataaa tactggggca gttttgccgg tttcgcctct ggctggaact
      421 actgggtact gtacgtttta gttgccatgg cagagctgac tgccgtgggt aaatacattc
      481 agttctggta tccggaaatc ccaacctggg tttctgccgc cgtgttcttt gtggtgatca
      541 acgccatcaa cctgacaaac gtaaaagtgt ttggcgagat ggagttctgg tttgccatta
      601 tcaaagttat cgcggtggta gcgatgatca tcttcggcgc ctggctgcta ttcagcggca
      661 acggcggccc gcaggcgagc gttagcaacc tgtgggatca gggtggtttc ctgccacacg
      721 gcttcaccgg tctggtgatg atgatggcga ttatcatgtt ctctttcggt ggtctggaac
      781 tggtggggat caccgcagca gaagctgata acccggagca aagtatcccg aaagcaacta
      841 accaggttat ctaccgcatc ctgattttct atattggctc gttagccgtt ctgctctcac
      901 taatgccgtg gacccgcgtt accgccgata ccagtccgtt tgtgctgatc ttccacgagt
      961 taggcgatac ctttgtagcg aatgcgctga atatcgtggt actgactgct gcgctctccg
     1021 tgtacaacag ctgcgtatat tgcaacagcc gtatgctgtt cggtctggca caacaaggga
     1081 acgcgccaaa agcgctggcg tctgtcgata aacgcggcgt accggttaac actattctgg
     1141 tgtctgcgct ggttacggca ttgtgcgtac tgattaacta ccttgcccca gagtccgctt
     1201 tcggactgtt aatggcgctg gtggtatctg cactggtcat caactgggcg atgatcagtc
     1261 tggcgcatat gaaattccgt cgtgccaaac aagaacaagg cgtggtaact cgcttccctg
     1321 ccctgcttta tccgctgggt aactggatct gcctgctgtt tatggcggcg gtactggtga
     1381 ttatgctgat gaccccagga atggcgattt cggtatacct gatcccggta tggctgatcg
     1441 tgttaggtat cggctatctg tttaaagaga aaaccgccaa agccgtaaaa gcgcattaat
     1501 ctctctacgc cctcacccgt gcagggtgag ggcaataatc tttacaccaa cgccccgtaa
     1561 atcgtcagta gcgcaattac caccacgacc acgaacgagg ttttcttcgc cattgaaacc
     1621 gctgcctttg gcgtctccac cttatcgaca tgcggttcac gcgccagaga gaactgtgcc
     1681 agacgcgtta atacctgata ttgcgaagta tggaaatcac ccagcgaagc aaaccaggcc
     1741 ggtaacgctt tctcaccatg accgatcaag gcatatacca cacccgcaag acgaaccggc
     1801 acccaatcca gtacatgaag cacgccatca atgccggact gtaaacgatg atgcggcgtc
     1861 tggtatcgcg ccagccagta ttgccatgca cgcaaaaacg cataccccat cagcgtaacg
     1921 ggtccccagg ttccccccac aatcagccag aacagcggag caagataaaa acgaaagtta
     1981 atccacagca atgcattttg cagctcacgc aaatactcac gttcatcgca gcctgccggg
     2041 acgccgtgaa tcatggtgag ttcgccagcc atcgtggcac gggcatggct atcattacgt
     2101 gaagcagctg tcagataagc atgataatga agacgaactt tacctgcgcc aatacacagc
     2161 aaaccaatca gcagccacac cagcagcgtg ggaacgttga acaatactcc ctgcaatgcg
     2221 cgtaacagta aaaaagtcac gcccatcgca ataatggtca tgcctaatgt gcgcccgaga
     2281 gaaaaatgtt tcacccgccg aaagaacgct tcaagacgat gatcaagctg ccagtgctcg
     2341 cccaacttaa acaggcgctc gaaaattaac accagtaagg ttgtaaatag cgtcatgttg
     2401 tctccttgct gaccagtgca cgaaaccgtg cccaatcaaa tgcagggccg ggatcggttt
     2461 tccgatccgg cgcaatatca caatggcccg tcatgttatt agcgatatct ggatagcgat
     2521 caatcagtgc ccgcgtaacc gccgcaagct gttgatactg cgcatcggta tacgccagcg
     2581 tatcggtgcc ttccagctca atcccaatag aaaaatcatt gcagcgttca cgcccctgat
     2641 actgagagac tcccgcatgc caggcacgtt tatcgaaagg aacatactgg actatttcac
     2701 catcacggcg aatcaaacag tgagcggaga cgcgcaaatg ggcgatctca gcaaagaaag
     2761 gatgtgcctg cggatcaata gttccagtga ataatgcgtc gatccacgga ccgccaaact
     2821 cgcctggcgg caggctaata ttgtgcacca ccagcagggt gggtgtttcg tcatccgggc
     2881 ggcaatcgta atgtggtgag ggaacgcggc gcgcgccaac cagccacccc tgttctaaca
     2941 acatgcagga tctccttata tgtggtgcta atacccggtt cagagtagca tgtttctacc
     3001 ttatgattcg ttagctatct ggagttttaa catgccgcct cgccgctata accctgacac
     3061 ccgacgtgac gagctgctgg aacgcattaa tctcgatatc cccggcgcgg tggcccaggc
     3121 gctacgggaa gatttaggcg gaacagtcga tgccaacaat gacattacgg caaaactttt
     3181 accggaaaac tctcgctctc atgccacagt gatcacccgc gagaatggcg tattttgcgg
     3241 caaacgctgg gttgaagagg tgtttattca actggcaggc gacgatgtca ccattatctg
     3301 gcatgtggat gacggcgatg tcatcaacgc caatcaaccc ttgttcgaac ttgaaggccc
     3361 atctcgcgtg ctgttaacgg gcgaacgcac tgcgcttaat tttgtgcaaa ctctttcagg
     3421 agttgccagt aaggtacgcc actatgtcga attactggaa ggcaccaaca cgcaactgtt
     3481 ggatacgcgt aaaaccttgc ccggcctgcg ttcagctctg aaatacgcgg tactttgcgg
     3541 cggcggagcg aatcaccgcc tggggctttc tgatgccttc ctgatcaaag aaaaccatat
     3601 tattgcctcc ggctcagtgc ggcaggcggt cgaaaaagcg tcctggttgc acccggatgc
     3661 gccagtagaa gtcgaagtag agaatctgga agaacttgat gaagctctga aagcaggtgc
     3721 cgatatcatc atgctggata acttcgaaac agaacagatg cgcgaagccg tcaaacgcac
     3781 caacggcaag gcgctactgg aagtgtctgg caacgtcact gacaaaacac tgcgcgaatt
     3841 tgccgaaacg ggcgtggact ttatctccgt tggtgcgcta actaaacacg tacaggcact
     3901 cgacctttca atgcgttttc gctaattgct gacagattta tccgctccat cgcgggcgga
     3961 taaatctccc cttccccaac tttcttcgta acacctcgca aatttcttgt ctttcgctgc
     4021 aacgaagtaa aaaacctcgg aagctgcttt cccgtttcgt tttttgcccc tcgcttgtgc
     4081 tgatggcgtt tgccaaagta gcgccaacca aatcaaggag cgaaacagat ggacaagcaa
     4141 cgcggtttta cacttatcga actgatggtg gttattggca tcattgccat tttaagcgcc
     4201 attggtattc ccgcttatca aaactacctg cgcaaagccg cactcaccga catgctacaa
     4261 acctttgtgc cttaccgtac cgccgtagag ttgtgcgcgc tggaacatgg tggattaaat
     4321 acctgcgacg gtggcagcaa tggcattccc tcgcctacca ccacccgcta tgtttcagcc
     4381 atgagtgtgg taaagggcgt ggtgtcgctg accgggcaag aaagtctcaa tgggctaagc
     4441 atcgtcatga caccgggttg ggataacgca aacggcgtca ccggctggac gcgtaactgc
     4501 aatattcaaa gtgacagcgc attgcagcaa gcctgcgaag atgtctttcg ctttgatgac
     4561 gcaaactaag gagcggcaat gaatattcca cagctcaccg ccctgtgtct gcgttatcag
     4621 ggagtcttgc tggatgccag cgaagaggtg gttcatgttg cggtagtcga tgcaccttcg
     4681 catgagctac tggacgcatt gcatttcgct accaccaaac gtattgagat cacctgctgg
     4741 acgcgccaac aaatggaagg tcacgccagt cgcacacaac agacattgcc cgtagctgtt
     4801 caggagaagc atcagcccaa agcagagttg ctgactcgaa cgttacaatc tgcgctggaa
     4861 caacgtgcgt ctgatattca tatcgaacca gcggacaatg cctaccgcat ccgcttgcgt
     4921 atcgacggcg tattgcatcc tttaccggat gtttcaccgg atgccggagt cgcattaacc
     4981 gccagattaa aagtgctggg aaacctggat attgcggaac atcgcctgcc gcaggacggg
     5041 caattcactg tcgaactggc aggaaacgcc gtctcatttc gtattgcgac cttagcatgt
     5101 cggggtggtg aaaaggtggt attaaggttg ttacagcagg tgaaccaggc actggatgtt
     5161 aacacgcttg gaatgcagcc gtcacaactg gtggactttg ctcatgcctt gcaacaacca
     5221 cagggactgg tgctggtaac tggccctaca ggcagcggca aaacggtcac gctttatagt
     5281 gccctgcaaa tgctgaatac cgctgacatt aatatttgta gcgtcgaaga tccggttgag
     5341 atccccatag ccggactaaa ccagacgcaa atccattcgc gtgccggact cacctttcag
     5401 ggcgttttgc gtgcgttatt gcgccaggat cctgacgtca tcatgatcgg agagatccgc
     5461 gatggcgaaa cggcagaaat tgccattaaa gccgcgcaaa ccggtcacct ggtgttgtct
     5521 accctacaca ctaattccac ctgcgaaacg ctggtacgtt tacagcaaat gggagtcgcc
     5581 cgctggatgc tctcatcggc gcttacgctg gtaatagccc agcgtctggt acgcaaactt
     5641 tgcccgcatt gtcgccggca gcaaggggag cccatccata ttccagacaa tgtatggccg
     5701 tcgccgctgc cccactggca ggcacccggt tgtgtacatt gctaccacgg tttttatggt
     5761 cgcacggcct tatttgaagt tctgcccata acaccggtca tacgccagct tatttccgct
     5821 aataccgacg ttgaatcgct ggaaacgcac gcccgacagg cgggtatgcg aacgcttttt
     5881 gaaaacggct gcctggccgt ggagcaaggc ttaaccacct ttgaagagtt aatccgcgta
     5941 ttggggatgc cgcatggcga gtaagcaact ctggcgctgg catggcataa ccggcgacgg
     6001 caatgcgcaa gatgggatgc tatgggcaga gagccgtgct ttgctgctca tggcactaca
     6061 gcaacagatg gttaccccac taagcctgaa gcgaatcacc atcaattctg cgcagtggcg
     6121 aggagataaa agcgcggaag tcattcatca actggcgacg ctactcaaag ccgggttaac
     6181 gctttctgaa gggctggctc tgctggcgga acagcatccc agtaagcagt ggcaagcgtt
     6241 gctgcaatca ctggcgcacg atcttgaaca gggcgttgcc ttttccaatg ccttattacc
     6301 ctggtcagag gtatttccgc cgctctatca ggcgatgatc cgcacgggtg aactgaccgg
     6361 taagctggat gaatgctgct ttgaactggc gcgtcagcaa aaagcccagc gtcagttgac
     6421 cgacaaagtg aaatcagcgt tacgttatcc cattatcatt ttagcgatgg caatcatggt
     6481 ggttgtggca atgctgcatt ttgtactgcc ggagtttgcc gctatctata agacctttaa
     6541 cactccgcta ccggcgctaa cgcaggggat catgacgctg gcaagtttta gtggcgaatg
     6601 gggttggttg ctggtgctat tcggctttct gctggcgata gccaataagc tgctgatgcg
     6661 ccacccaacc aggcttatcg cacggcagaa attgctgtta cgcatcccga ttatgggttc
     6721 actgatgcgg ggacaaaaac tcacgcagat ctttacgatt ctgacactga cacaaagtgc
     6781 aggcatttct tttttacagg gcgttgagag cgtcagagaa acaatgcgct gcccgtactg
     6841 ggtgcaactt ctgacacaaa tccagcacga tatcagtaac ggtcacccca tctggctggc
     6901 gctaaaaaat gccggtgagt ttagcccgct ctgtttgcaa ttagtgagaa caggagaggc
     6961 atccggctcg ctggatctca tgttagacaa cctcgcccat catcatcggg ataacacaat
     7021 ggcgctggcg gataacctcg cagccttact ggaaccggcg ttgctgatca taacgggagg
     7081 aattatcggt acgctggtgg tggcaatgta tctgccaatt ttccatttag gcgatgcgat
     7141 gagtggaatg ggataacgct ggcgtgttgc tccacgccag cgttggtata ttacaggttg
     7201 ttgaagatgc ggttttcttg ttcctgcaca cggataaacg tggtgcgctt ggtcagttct
     7261 ttcaggcgtg aagccccaac gtatgtacaa gctgaacgca ggccgcccaa aatatcacgc
     7321 gcggtatttt caaccgggcc tcgcagcggc agcttaacgg ttttaccttc tgctgcgcga
     7381 tattccgcaa cgccgccaac gtgacgtttc atcgcagact ctgagctcat gccgtagaac
     7441 aacataaatt tctcgccgtt ctcctcaacg atgcgaccgc cgctctcttc gtggcccgcc
     7501 agcatgccgc caagcattac gaaatcggca ccgccgccaa aggctttcgc gacatcgccc
     7561 ggcgtggtgc agccgccatc gctgacgatc attccgccca gaccgtgcgc agcatcggcg
     7621 cattcgatca ccgcagaaag ttgcggataa ccgacgcctg ttttgacgcg agttgtacaa
     7681 acagaacctg ggccaatgcc aactttaacg atatcggcac cggagaggat aagctcctca
     7741 cacatttcac cagtcactac gttgcctgca caaatggttt tggtcggcca cgcttcacgc
     7801 gctttcgcaa cgaactgcac gaagtgttcg gaataaccat tcgccacgtc aatacaaacg
     7861 aaatttaatg ccgggttcag gtcgagaatc tgtttagttt tttcgaaatc cgcatcagac
     7921 gtgccggtag aaaccatcac atgtttcagc acatcagcgg aagaattgtt gataaacgct
     7981 tgccactctt cgacagaata gtgtttatgc acagcagtca gaatatcaaa agaagccagc
     8041 gcagaggcca tagaaaatgt gcctacggtg tccatatttg cggcaataat cggcacgccg
     8101 gaccaactct gacctgaatg tttgaaggtg aattgacgtt ccagttcaac atcggaacgg
     8161 cttttaagag tggagcgttt agggcggatg agaacgtctt taaaacctaa cttcagatct
     8221 tcttcaatac gcatgtgcgg attcctgggg ttaatggcta taagggtaag agcgcaactc
     8281 cagtgacgtt atcatacgca ctaatcaatg cggcgcaaga ctgcgaaatt gtcttttttt
     8341 acgctacaat cccataaatt tacctggtaa atagtgagcg aacgtttcat caatttcgct
     8401 ttcgtgcgct catttttaac gttttgataa tcatgatgaa ttcccgggaa tataaattat
     8461 gaggtatata gttgccttaa cgggaggcat tggcagtggc aagagtaccg ttgccaatgc
     8521 gtttgctgat ctcggaatta acgtcattga tgccgatatt attgcgcgtc aggtggttga
     8581 accaggtgca cctgcgctac atgccattgc tgatcacttt ggcgctaaca tgattgctgc
     8641 tgatggaaca ttgcagcgcc gggccttgcg cgagcggatc ttcgccaacc cggaagagaa
     8701 aaactggctt aacgccctgc tgcatccgct gattcagcaa gagacgcaac accagatcca
     8761 gcgagctact tccccctatg tactatgggt tgtgccattg ctggtagaaa actcactgta
     8821 taaaaaagcg aatcgagtgc tggtggtgga tgtcagccca gaaacgcaac ttaagcgcac
     8881 catgcagcgc gacgatgtaa ctcgcgagca tgtcgaacaa atccttgctg ctcaggcaac
     8941 gcgcgaagct cgccttgccg tggcagatga cgtcattgat aataacggcg caccggatgc
     9001 tatcgcatcg gatgttgccc gcctgcacgc acactatttg cagcttgcgt cgcagtttgt
     9061 ctcacaggaa aaaccgtaat gcagacccag gtcctttttg aacatccact aaatgaaaaa
     9121 atgcgtacat ggctgcgcat tgagtttttg attcagcaac tcaccgttaa tttacccatc
     9181 gttgaccacg ctggcgcgct gcatttcttc cgtaatgtca gcgaattact ggatgttttc
     9241 gagcgcggcg aagtccgcac tgagctgttg aaagaacttg accggcagca acgtaaactc
     9301 cagacctgga ttggcgtgcc cggcgtggac cagagccgta ttgaagcatt aattcagcag
     9361 ttaaaagcgg cggggagcgt attaatctcc gcgccgcgta tcgggcaatt tctacgtgaa
     9421 gatcgtttga ttgctctggt gcgtcagcga ctgagcatcc cgggcggctg ttgcagcttt
     9481 gatttaccta cattgcacat ttggctgcat ctaccccagg cgcagcgcga cagccaggta
     9541 gaaacctgga ttgccagcct gaacccgctt acccaggcac ttaccatggt gctggattta
     9601 attcgccagt cggccccctt ccgtaaacaa accagcctga atggttttta tcaggataac
     9661 ggtggcgatg ccgacttgct gcgcctgaat ctgtcgctcg attcacagct ttatccgcaa
     9721 atttccggtc ataagagccg ttttgccatt cgttttatgc cgctggacag tgaaaacgga
     9781 caggtaccag aacgtctgga tttcgaactg gcctgttgct aaggagtaaa aatgtcagaa
     9841 actattacgg tgaattgccc aacctgcggg aaaacggtgg tgtggggtga aatcagcccg
     9901 tttcggccat tttgctccaa acgttgtcag ctgatcgacc tcggagaatg ggctgctgaa
     9961 gaaaaacgaa tccccagcag tggcgatctt tccgaaagcg atgactggag cgaagagcca
    10021 aagcagtgac attctatctg atgcgatgct ggcctgataa aacgcgccag cgtcgcatca
    10081 ggcatcatat gcaaatgtcg gatgcagcgt gacgccttat ccggcataca cacgcttaag
    10141 tttcgcaatt accggttcat tggctggcgg aaaatcatcg gcattaagac cgaccagcga
    10201 catccactca ccgggttgcc cttctttacc ccacggctcc ccttcccagc tttcgaccag
    10261 ccagaaccac agagttatat gcctgtccgg gaattcatat tccagttttt caaatagcga
    10321 aaaatgttgt ggggtaatcc cgacttcttc ctgaagttca cgcaccaccg cctgttccgg
    10381 cgtttcgccc atttcaactt taccgccggg aaactccagt ttattcgcca tgtgcgcatc
    10441 tgctgcgcga cgcgttataa agatttcatt gttctcgttg cgaataatac ctaccgcaat
    10501 ttgcagcttt ttcattgtct taaacctata aaaaaggcgc agaatcctgc gccttttact
    10561 tcaactgtta gcttttattg caggcggcca tggcactgct tgtatttttt accagaacca
    10621 cacgggcaag gatcgttacg tcctactttg cgttcaccgg tttgcgccgc cagtgcagct
    10681 gcggctgcag agtcgtcatc ctgatggcta agctgctgca tttgcgctaa acgctcggct
    10741 tccatacgac gctgttgttc cagctcctca acctcttcag gcatacgtac ctgaactttg
    10801 ctcagcgtac tgataacttc atatttcaac gactccagca tcgctgcaaa catggagaac
    10861 gattcacgtt tgtattcctg cttcggatct ttctgtgcat agccacgcag gtggataccc
    10921 tgacgcagat agtccatcgc tgccaggtgc tctttccaca gagagtcgag agtttgcagc
    10981 atgacgcctt tctcgaagtg acgcatcatc tcagcaccaa ccacttcttc tttacgctga
    11041 tacacttcga tggactgcgc cagaatgcgc tcacgcagcg tctcttcatg cagttctggt
    11101 tctttatcca gccactcggc aattggtaaa tcgaggtcga aatcgttctt cagacgttcc
    11161 tgcagccccg gaatatccca catttcttcc agcgactgtg gcggaatgta ggcatcaatg
    11221 gtcgctttga acacatcttc acgaatgctg ttgatggttt cgctcacatc gctgacatcc
    11281 agcagttcgt tacgctggga gtaaatggcg cgacgctgat cgttagccac gtcatcatat
    11341 tccagcagtt gcttacgaat gtcgaagtta cggctttcaa ctttacgctg ggcgttggca
    11401 atcgctttgg tcacccacgg gtgctcaatg gcttcgcctg gcttcatacc cagtttacgc
    11461 atcatgccgg atactcggtc ggaagcaaaa atacgcatca gcgcatcttc catcgacagg
    11521 tagaaacggg aagaaccagc atccccctga cgaccagaac gaccgcgcag ctggttatcg
    11581 atacgacggg attcgtgacg ttcagtaccg atgatatgca ggccacctgc tgccagtacc
    11641 gcatcgtgac gtacctgcca gtcggcttta attttttcaa tttgctctgc agtcggattt
    11701 tccagcgcgg caacttctgc ctgccagcta ccaccgagca caatatcggt accacgaccc
    11761 gccatgttgg tcgcgatagt caccgcagcc gggtaacctg cctgagcaac aatcgccgct
    11821 tcgttggcat ggaatttggc gttcaggacg ttgtgcttaa taccggcttt ggtcagttca
    11881 tttgacacca gctccgattt ttcgatggag attgtaccca ccagcaccgg ctggcctttc
    11941 gcagtacgtt ctttgatatc ttcaatgatc gcctgaattt tttccgcttc agtcatgtag
    12001 accaggtccg gcaaatcttt acgaatcatt ggacggttgg tcggaacaac gacagtatcc
    12061 agcttataga tggagctgaa ttcgaaagct tcggtatcag cagtaccagt catccccgcc
    12121 agtttttcat acagacggaa gtagttctgg aaggtgatcg aagccagcgt ttggttttcg
    12181 ttctggatct gcacaccttc tttcgcttcc acagcctggt gcagaccatc ggaccagcga
    12241 cggccctgca tggtacgacc ggtgtgttcg tcaacgatga taacttcacc atctttaacg
    12301 atgtagtcga cgtcgcgggt aaacagcgca tgagcgcgca gcgccgccgt tacgtggtgc
    12361 atcagcatga tgttggccgg agagtacaga gactcacctt catccatgat gccttcttta
    12421 accagcagtt cttcaatcag aaccagacca cgttcggtca ggttcacctg gcgagatttt
    12481 tcatccaccg agaagtggcc ttcgccctgg aaggtttcgg agtcttcttt ttcctgacgg
    12541 atcaggtgcg gaataatttt attcacgcgt ttatacattt ccgagctgtc ttctgctggg
    12601 ccggaaatga tcagcggtgt acgcgcttca tcgatgagga tggagtccac ttcgtccacc
    12661 agcgcatagt gcagtttacg ttgtacacgt tcttcagggc tgaatgccat gttgtcgcgc
    12721 aggtagtcaa agccgtattc gttgttcgta ccgtaagtga tgtcagcagc gtaggcttca
    12781 cgctttgccg gtgctggcat gcccggtagg ttgataccga cagtcaggcc aaggaattca
    12841 aacagcggac ggttgttttc ggcgtcacgt tgcgccaggt agtcgttgac ggtaactacg
    12901 tgtacgcctt taccggttag tgcgttcagg taagcaggca gcgttgcggt cagggtttta
    12961 ccttcaccgg tacgcatttc ggcgatgcag cgttcgttaa gaaccatacc gccgagtaac
    13021 tgaacgtcga agtgacgcat gccaaagacg cgcttacttg cctcacgcac cacagcgaaa
    13081 gcttccggga tcagattttc cagcacttcg cctttttcca gacgcgcacg aaactctgcg
    13141 gttttccctt tcagttcttc gtcggagagt ttttccatct ccggttccat ggcattgatg
    13201 atgttgacca ctttgcgcat ccggcgcagg gtgcgatcgt tacgactacc gaaaacttta
    13261 gttaataatt tgattagcat aataaaatct caaacgcccc gcgttgcgga gttaataaaa
    13321 tgaggtaaag gtttattgtt gttagctgag gcgttgaggg ccagcacgga tgccttgcgc
    13381 ctggcttatc cagacgggcg tgctgaattt tgcttgtggg gtaaaatgcg cataatcaat
    13441 gcgataaccc ttttcagacg gcgtgccttc ctgggtcaga agcgcgctaa gcgtatccag
    13501 taatgcaaga tgttgcgcct gaagaggcaa agattcttca gcaacgggca gcgtttgcgg
    13561 tgccatcgcg aaggaaagat ggcggattac cgtgcgaatg gcatgttgat gccagtaatc
    13621 aacggaatag ttcgaattcg ggcggcgcgt gttcgcttcc agcaaggcca attgaccaaa
    13681 gttaactttg gctgaaggct cgtggttgcg ggttgtcgct tttgcgggcg cgtttggttc
    13741 ggcggcattg ctgagtgcag gcaaacctaa actcgccgcg accatcccta ataagagatg
    13801 cggccagaag tagcgtttac caaactgtcg ccagcgcgtc agtattccac tcacgttatt
    13861 gccatcccga tcgtattcaa acgtatttag cgcacaaatc ttatcattaa agcctgaagt
    13921 ccacagcacg aatgaagata agttgtgcgt aaaagtgctt aagaaaacag cgttcgcacc
    13981 gttttttata gctcttataa aattcaaaaa aaaaacgact ggttcacctg gccggagaga
    14041 gtgccagatt tgccagtcga attttatacg acagtataaa tgtcgttatg ccagtacggc
    14101 tgaaggcgct ttgaaggcca acggcagttc tgcgtcgtcc tggaaggtca catattccca
    14161 ggcttcctgt ttcgccagga cagcctgcag cagtttgtta ttcagtgcat gaccggattt
    14221 ataagcggta aatgcaccaa taatattgtg accacacatg aacaagtcac cgatcgcatc
    14281 aagcattttg tgacgcacaa attcgtcttc aaaacgcagg ccgtcttcgt tcagtacgcg
    14341 ataatcgtca acaacgatgg cacaatcgaa gctgccgccc aggcacaaac cacgggactg
    14401 cagatattcg atatcacgca tgaaaccgaa cgtacgcgca cggctgatct ggcgcataaa
    14461 tgcatcagcg gagaagttca tcgcatagcg ctggttgctg gaatcaatag ccggatggtt
    14521 aaaatcgatg gtgaaatcca gagaaaaacc attgtacggc ttaaattcag cccacttatc
    14581 gccatcttcg acacgaacag tttctttaat gcgaacaaat ttcttggcgc agttcaactc
    14641 gtcgataccg gcgtcaagca gcaggtatac aaacggagcg gcgctgccgt ccatgatcgg
    14701 gatttccggc gcgttaactt cgataacaat gttatcgatg cccaggcccg cgagagcagc
    14761 attgaggtgc tctacggttg aaatccgcac atcatgctcg ttgaccagac acgtacagag
    14821 catggtatca cgcacagatt tggcatcggc cgggaaatct accggtggat tcaagtcagt
    14881 gcgacgatag atgaccccgg tgttggccgg cgcagggcgt aacgtcaggg tgactttctt
    14941 gccggtatgt aaaccgacac ccgtcgcctg aacgatacgt ttaagtgtcc tttgtttgat
    15001 catcgtatta tctcgccaaa ttacctatcc aaccgaagtg tactatacat tcggcgggcc
    15061 agtttagcac aaagagcctc gaaacccaaa ttccagtcaa ttcttaatca gcttgcttac
    15121 gcaggaatgc tgggatatcc agataatccg gctctttcgc agtttgcggc gcattgtcat
    15181 tcacgacttt agcaaccggc ttctgctcct gggtcagcgg agccataccg tgctgctggt
    15241 agcgatccat cactggctgc tgaacctgct tattggtcac cagagtgatt tcaggacgtt
    15301 tgtccatgcc gatacctgtc gcaacaacag ttacgcgcag ctcgtcattc atatccgggt
    15361 caagagaagt accgataacc acggtcgcgt tgtcggaagc aaatgcacgg atggtgttac
    15421 ctaccgtttc gaactcatcc agacgcaggt cgaagcccgc tgtgatgttt accagtacgc
    15481 cgcgtgcgcc agacaggtcg atatcttcca gcagcggaga agagatagcc atttcagcag
    15541 cttcttccgc acggtcttca ccgctcgcca cgccagaacc catcattgcg tagcccattt
    15601 cagacattac ggtgcgtacg tctgcaaagt ccacgttcat caaacccgga cgagtaatca
    15661 gttcggcgat accttgcaca gcgcctttca gtacatcgtt cgctgcgcca aacgcatcca
    15721 gcagggagat accgcggccc agaactttca gcagtttgtc gttcgggata gtgatcagag
    15781 agtccacatg cttggacagt tcagtgatcc cctgctccgc gaatgccata cgcttcttgc
    15841 cttcaaagtt gaaaggctta gtgacgacag caacggtcag gatacccaaa tcttttgcta
    15901 cttcagcgac gactggtgct gcacctgtac cggtaccacc acccataccc gcagcaataa
    15961 agaccatgtc tgcaccttcc agcgccgcac gcagtgcatc gcgatcctca tcagccgcat
    16021 tgcggccaac ttctggatta gcgccagcgc ccagtccttt ggtgataccg ctaccgattt
    16081 gaatcgtctg tccaaccgct gttttacgca gcgcttgtgc atcggtattt accgcgaaga
    16141 attcaacacc ttcaatgcgc tcacgcacca tgtgttcaac agcattaccg ccgccgccgc
    16201 cgacgccgat gactttaatc accgcgtcat tggtaagttc cattggttca aacatagttt
    16261 ctctccgatt tgtgcctgtc gcctgaggcc gtaatcatcg tcggcctcat aaaaattaaa
    16321 actcttttcg cagccaacta ttgagtcgct tgatccacga gccaactgat gctgtaacac
    16381 gtttttctac ttcagcttca ccgttaagat gtgactcttt cccatagtga agcaatccca
    16441 ccgccgtcga ataatacggc tcctgagcat aatccgttaa accggtaata ttcagcggcg
    16501 cgccgatacg cacttgcgta tgaaacacgc gctgagcaca ggctgcaaga ccttcgatct
    16561 gcgctgcgcc accggttaat acaatgcctg ccgccaggtg atgtttaacc ccttgttggc
    16621 gaagcttttc ctgcaactgc aatatctctt cgttgaccag attgagcagc tcggtatagc
    16681 gcggctcgat cacctctgcc agggtctgac gttgtagact ccgcggcgga cgaccgccta
    16741 cgctcggcac ttccacgctc tcatcttttc caacgatgga gcccagcgca caaccgtggc
    16801 gaactttaat cgcttcggcg tcgcttggcg gtgtgccaaa ggcgtaagcg atatcactgg
    16861 tcacgacatt gccagcataa ggaattacct tcgtgtggcg caatgcccca ccggtataaa
    16921 cggcgatatc cattgtacca ccaccgatat cgacgacgca gacacccagt tcacgttcat
    16981 cttccgtcaa taccgaataa cttgatgcca gtccggcaaa tatcagttgg tcaactttca
    17041 gcccacaacg ttcaaccgct ttgacgatgt ttttcgccat atcgttgtga catgtgatca
    17101 ggtgcacttt tgcctgcatc cgcacgcccg aaagccctac cggattcttg atcccttcct
    17161 gatagtcaat cgcatactct tgcgggatca catgcagcac acgatgctca tcgcgcacac
    17221 gcaccgattt cgcggtatgg acgacgtttt ccacatcttc ttgcgtcact tcttcttcag
    17281 aaataggcac cataccaatt tcattctggc agctgatgtg cttaccagaa agcgccagat
    17341 ataccgaaga gatctgacaa tctgccatca attctgcctg gtcaatggcg cgttgtacgc
    17401 acttgaccac ggattcgagg tcgttcaccc cgcctttatc cataccacgc gacgggcagc
    17461 tgcccacgcc aatgatattg accataccgt cgggcagaac ttcccctact aaagcggcaa
    17521 ccttcgcggt accaatctcc agtcctacta ccagttttct gtccgtcgcc ttgatcattg
    17581 ttgttctgcc tgtgcctgat tttgttgctg agtagattcc cctggcggca agggcgccca
    17641 gcctactgcc gctccagagt cataacgcaa atcaacgtag ctaatccgtt tgccatcggt
    17701 ttgcgcctgc tgctgtaaaa ccggataaag ttctacaaag cgagccaaac gtttcatcgt
    17761 atcgccccga ccaagattga gcttaatatc gttattcagc gtcaactgcc aggaacgtcg
    17821 cgcggtcatc gccgcttcct tcagagtaaa tctgtccttt gccagcatct gccccatttc
    17881 gcgatagccc tgcaacactt cattggcgct gccttccggg ccatacagca ttggaagcac
    17941 ctgcttgctg gtgcgatctg gcggcacgct gaaggtgttt ccttccgcgt ctaccatatg
    18001 ttgatcattc caccgcgcaa tcggcacata ttcaaccaga tgaatcttca attcatcagg
    18061 ccactgcttt ctgacgctca cctgcttaat ccacggcagg cgttgttcta tttgcgtctg
    18121 gatgatgttg acatcctggg tcataaaggt acctggctca cccaatgcca ggatcgactg
    18181 ccggatatcg tcattacgcg tgtaatggcg ttcaccggtc aacaccagct ttgagagcgg
    18241 caggcgttgc gcatcttcca tccagcccaa cacgacccag ccgctcacca acactgtcgt
    18301 taaaacggtc agcaggaaaa ggatccccgc cagacgcgtt ccattattgc ggcgagaaga
    18361 aacctcttct tcgctatttc gcgtgttcag agcagcctgc gacatattag tccgccagtt
    18421 ccagaattcg tactaccaac tgcgagaagc tcatacctgc ctgacgtgcc gccatcggca
    18481 ccagactgtg gctggtcata cccggtgagg tattggcttc cagcagataa aactgtccat
    18541 cactgtccag cataacgtca atacgtcccc atcctttgca acctaacgtc gtccatgctt
    18601 ttagcactaa tgcctgcaaa ttggcctctt gtgacgcttc cagacctgcg gggcagaaat
    18661 actgtgtctc atcagagaga tacttcgcct cataatcata gaaggttccg gccggttgaa
    18721 tacgtattga cggtaaaatt tcttcaccga gtatcgcaac cgtgaactcc ggcccactta
    18781 gccatttttc aatcaatact tcttcatcgt gctgaaatgc caatcttaat gcatcttgta
    18841 gagcattttc tgctactact tttgacattc ccacactgga accttcgcgg ctcggcttaa
    18901 cgataaccgg caaacccaga gaagaaattt ctgctaactg cttatcgtta aggccttttt
    18961 caaactctgc gcgggttaac gctacccacg gcgcgaccgg taaaccggca ccctgccaca
    19021 gaagtttgct gcgtagttta tccattgaaa gcgcagatgc catcactccg ctgccggtat
    19081 aaggcaagcc catcagctcg agcatgccct gcagcgtacc atcttcaccg ccgcgaccgt
    19141 gtagcgcgat aaacactttc tgaaaaccca tcgacttcag ttgcgtcacg tcgacttctt
    19201 tcgggtcgac aggatacgcg tcaataccgc cttcacgcag cccagctaac accgctgcgc
    19261 cagaattcag agaaacttcc cgctcagcgg aggtcccacc caacaggacc gcgattttat
    19321 cagtcatgtt gttcttcctc cggagtttgc ggcttcagtt tgatttcagc taaagaacga
    19381 gcaatttttc cgatattacc agccccctga acgagaatca ggtcgttacc ggttaatacc
    19441 ggtgccagca tctcggctac ctgcgccggg tccggcacca gaatgggatc aattttccca
    19501 cgtccacgaa ttgtgcgaca cagcgaacgg ctgtccgctc ccggaattgg cgcttcacca
    19561 gccggataca cttccagcat caacagggta tcaacctgcg tcagcacatt ggcgaaatca
    19621 tcatacaggt cgcgcgtacg ggtaaaacgg tgcggctgaa acagcattac caggttttta
    19681 tccggccagc ctgcacgcgc cgctttaata gtggcgtcca cttccgtcgg gtggtggccg
    19741 tagtcatcga ccagcattgc cgtaccgctt ttaccattca ctggctccag cgggaattca
    19801 ccgaggaaat cgaaacggcg ccccgtcccc tggaagctct ccagcgcacg caaaatagcc
    19861 tcgtcgtcaa ttccctcttc cgtagcaacc gcaaccgcag ctgcggcgtt cagcgcgtta
    19921 tgacgacctg gcgcattcag ggtgacgcgc atcggctctt tgtcctggcg cagcagcgta
    19981 aagtgcccct gcgggccaat ctgctgataa tcttctacac gcacgtcggc atcttcgctg
    20041 aagccgtaag tcgtggtctg acgccccaca cgcggtaaca attcgcggat caccggatca
    20101 tcaacacaca tcaccgcacg accgtaaaac ggcaggttgt gcagaaaatt aataaaagtc
    20161 tgttttaaat tctcaaagtc gccctggtag gtatccatgt ggtcggcttc gatattggtg
    20221 acaatcgcca ccatcggttg cagatgcagg aacgatgcat cactctcatc tgcttcggca
    20281 atcaggtacc gaccatgccc caaacgcgca tgaacccccg ccgcttttac cagcccgccg
    20341 ttaacgaagg ttgggtcgag ccccgcttct gcgtagatgc tggaaaccat cgcggtggtt
    20401 gtcgttttgc cgtgtgttcc ggcaatggcg atgccatgac gaaaacgcat taactcagcc
    20461 agcatttcag cacgacggat caccggaata cgcgcttcat gagctgcaac aatttccggg
    20521 ttatcggcag aaatcgcgct ggaaacaacg accacgctgg catcacgtac gttttccggg
    20581 cgatggttga aataaatcgt cgctcccaga ttcattaact gctgcgtgac cggatttggc
    20641 gctaaatcgg aaccactgat ctgataacct tcattggcca gaacttcggc aataccgccc
    20701 ataccggcac caccgatgcc gacaaaatgt atgtgccgaa cgcgacgcat ttcgggcacg
    20761 atggaacgca gttttgccaa ttgttgtgta ttcattcttt acgccattaa cttcttaaat
    20821 tcatacgatg caaaaggcat cgctacaatt acgcccgggc agcccggctc acttcatttg
    20881 ccactcgctc ggtggcatcc ggaatggatg cagcgcgggc gcgttctgcc atggttaata
    20941 aggtttctcg cgaccacccg gccagggtgt tggcgacagc atccacgcta agctgtgact
    21001 gctcgataat tttggctgcg cccgcttttt ccagcggtag cgcattccag tattgctgac
    21061 ggtctttatg ttgaaacggc acaaacaacg caggtagtcc tgccgcggcg atttcactca
    21121 ccgttaacgc accggagcgg caaacgacga catccgccca cgcatacgcc gccgccatat
    21181 catcaataaa ttccgtcact ttatgctgcg gttgccccgc ttcggcatac gcctgttcaa
    21241 cggattgttg cgaacctttg ccgctctgat gccagatagt gactgaatca cccagtttcg
    21301 cagcaacctg cggcattgtc tggttaagaa tgcgtgcgcc ctgggaacca cccactacca
    21361 acacacgaac cggaccttca cgtccagcca aacgttgctg cggcaacggc agcgccagca
    21421 catcggtacg caccgggtta cccacaactt ccgcattagg aaaagcacct ggaaacgcct
    21481 gcatcacttt ggtagcaatc ttcgccagcc atttattggt taagcccgca ataccgtttt
    21541 gttcatgaag tacaaccgga atgcctaacg accacgcggc cagaccacct ggacctgata
    21601 catagccgcc cataccgagc accacgtcag gtttgtacgc tttcataatc gcccgcgcct
    21661 gacgccaggc gttgaagata cgcagcggcg cagctatcag tgcttttata ccttttccac
    21721 gcagaccaga gatacgaatg aaatcaattt cgatgccatg ttttggcact aagtccgctt
    21781 ccatacggtc ggcagtcccc agccagcgaa cttgccaacc ctgagccatt agatggtgcg
    21841 caaccgccag ccccgggaat acatgtccac cggttccgcc tgccatcacc attaatcgct
    21901 tcccttgacc actcatcgtg aacctcgtac aaacgcctgc gctttctcca gacgcgtttc
    21961 ataatcaata cgcaacagca tcatgatggc tgtcgacata atcagtaagc tcgaaccacc
    22021 gtaactgatc agcggcaatg tcagaccttt ggtcggtaac atccccgccg ccgcgcctac
    22081 gttaaccagc gcctggaaac taaaccagat accaatagaa caggcgagaa aaccggaaaa
    22141 acggtggtca atttctaatg ctttgcggcc aatcgacatc gcgcgaaaag cgacgaagaa
    22201 taccattaaa agtgccagca ccacaccgac ataccccagt tcttcgccga taatggcgaa
    22261 aataaagtca gtgtgcgctt ccggcagata ctccagtttt tgtaccgagt tacctaaacc
    22321 ttgtccccac agttcaccgc gaccaaacgc catcaacgat tgcgttaact gatagccgct
    22381 gccaaaggga tcttcccacg ggttccagaa tgcggtaaca cggcggatac ggtacggttc
    22441 ggcgaggatc agcaacacaa ccgctgaaat gcccataccg ataatggcaa tgaactgcca
    22501 caatttcgct cccgccagga acaacatcgc cagcgtagtc acaaacaaca ccaccaccgt
    22561 accaaggtct ggctgtgcca gcagtaacac tgccaacacc agaattacgc ccatcggttt
    22621 cagaaagccg cgcaggttat tacgtacttc gtcgccttta cgcaccagat agttggcgat
    22681 atagcaaaac agcgacagtt ttgtcagctc cgcaggctgg atacgcagca aaccgagatc
    22741 gatccaacgc gatgcccctt taaccgagct acccactacc agaacgatca tcagcaggat
    22801 gatagatccg agcagcatcg tggcactgta gcgttgccag aactccatcg gcagacgcag
    22861 cgtaatgatc gccagaatga atgccaggat cagatagacg ccatcacgct tcgcgaagaa
    22921 gaacggatcg ttggttaagc gttgccctat gggcattgac gccgaggtca ccatgataaa
    22981 gccaatcgcc gcgaggccga aggtcagcca cagtaaggtg cgatcgtaca tgatcaggct
    23041 gtcggtatct ttttcccgcg agcccatcac ccagcccttt agcgccgtgg agatccagac
    23101 cagaatactg aatcctggca ggcgcggcat tttcaggcga gggagagata aacgcatcaa
    23161 cctaactcct tcgccagacg ggcaaactca ttgcctcgtt gttcaaagtt cttgaattga
    23221 tcaaggctgg cacaggccgg ggagagcaga accatatcgc ccggctgaac acgcgtagca
    23281 agcaagcgca tcgcctgttc catagtttcg gtttgttctg ccacttccgg gcgtagcgcc
    23341 gccagctgcg cgccgtcccg accgaaacaa tacagacgta cgttatcgcc attcaggtaa
    23401 cgcgccagtg ggctaaagtc cgccgattta ccatcgccac ccagcaacaa atgcagcgtg
    23461 ccgtctacat gcaggccatt cagcgccgct tccgtactgc cgacgttggt cgcttttgaa
    23521 tcgttaaccc agcgcacacc gttatgctcc agcacaactt caaagcgatg cggcagacca
    23581 gtgaatgtgg ttaacgcttt caggctgctg gcacgcggta accctgcggc atctgccagc
    23641 gccagcgccg ccagcgcatt ggtgtagtta tgctgcccgg aaagtttcat ttctttcacg
    23701 ttcagcactt tctcgccttt cacccgcagc caggtttcgc cctgctgatg attcaggtga
    23761 tagtcgccca tgttgacgcc aaagctgacg cagcgttcat ccgcaccgcg aatcggcatt
    23821 gttaaggcat catcagcatt aaccacgcaa acttttgcgt tttcgtaaat gcgcagtttt
    23881 gctgcacgat actgttgtaa accaaacgga tagcgatcca tatgatcttc agtcacgttc
    23941 agaatggtcg ctgctaccgc ctgtaagctg gaggtggttt ccagctggaa gctcgacagt
    24001 tccagcacgt acagttcgca ctcatcatcc aacagcatca acgcaggcag gccaatattg
    24061 ccacccacac caacgttaac ccccgccgct ttcgccattt cacccactag cgtggtgacc
    24121 gtgcttttgc cgttagaacc ggtaatcgcc acaatcggtg cttgtgcttc acgacagaac
    24181 agctcgatat cgccaacgat ttcgattccg gcatcagcgg cagcgcttaa ggatggatgc
    24241 gccagtgcaa taccgggact ggcgacaatc agatctgccg ccatcagcca ttcatcattc
    24301 agaccgcccg tgtggcgttc tacggcttcg ggtaatttat ccaggccagg cggtgtcata
    24361 cgcgtatcca taacgcgcgg cgtcacaccg cgagcgagga aaaagtccac gcaagaaagc
    24421 ccggtgaggc ccaggccgat aatgacgaca tttttaccct ggtaatcagc catgattaac
    24481 gtaccttcag cgttgccaga ccaatcagga ccagcatcag cgaaataatc cagaaacgca
    24541 caatgacgcg cggttccggc cagcctttca gttcatagtg gtgatgaatc ggtgccatgc
    24601 ggaaaatacg ctgtccgcgc agtttaaagg agccgacctg caggatgacg gaaagcgttt
    24661 ctaccacgaa cacgcccccc ataatcacca gcaggaattc ctgacgcagc agtacggcga
    24721 taatacctaa cgcaccacct aacgccagcg aacctacatc gcccataaag acctgcgccg
    24781 gataggtgtt aaaccacagg aagcccagtc ctgccccgac tatcgcggta cagacaatga
    24841 ccagttcccc ggcgtgtcgt agatacggta tgtgtaaata gctggcaaag ttcatgttgc
    24901 cggtcgccca cgccaccagc gcaaaaccac cggcgacaaa tacggtcggc ataattgcca
    24961 gaccgtcgag accatcggtc aggtttaccg cgttgccagt acccacaatg acaaagtaag
    25021 ccagcagaat gtagaacagc cccagttgcg gcatcacatc tttaaagaac ggaaccacca
    25081 gctgcgttgc gggcgtgtct ttgccggcaa ggtacagcgc gaaggcaaca cccagtgcaa
    25141 tgaccgacat ccagaaatac ttccaacggg caatcaggcc cttggtgtct ttacgcacca
    25201 ctttgcgata atcatcaaca aagccaataa caccgtaacc taccagcacc accagtacgc
    25261 accagacgta cggattggag gggtaagccc acagcagcac ggagatcaca atcgccgtca
    25321 ggatcattat cccgcccatg gtcggtgtac cgcgcttgct gaagtgtgat tcaggaccgt
    25381 cgttacgcac cacctgacca aaggaaagtt tttgcaaatg agcaatcata cgcgggccca
    25441 tccacaatga gatgaacagc gcggtcagca ggctgacgat ggcgcgaaac gtcagatagg
    25501 aaaagacgtt aaagccggaa taatatttga ccaaatgttc ggccagccaa actaacatgt
    25561 cccattctcc tgtaaagcgc gtactacctc ttccatggcg gcactacgtg aacccttaac
    25621 taaaatcgta attacctgtt gctcagcaat cagtgattta agacgcgcga taagcgcagt
    25681 tttatcggta aaatgttcgc caacaccgct ggcagtgctg atagcatggc tttgtttacc
    25741 cacacttaac acgcagtcaa taccagcggc ttttgccgct tcgcccacct gtacatggca
    25801 ggcttcgctt tcagcaccca gttccgccat atcgcccacc accagcacgc ggtagcccgg
    25861 catttcagcc agaacctgaa cagcggcggt cattgaaccg acattggcgt tgtaggagtc
    25921 gtcgagcagc aactggtttt ctgccagttt tatgggaaac agacggcctg gaacagcttt
    25981 cagatttgcc agccccgctt tgatagcatc aagcgttgcg cccacggaca tggagagcgc
    26041 agcggctgcc agcgcattcg caatattgtg acgccccggc aacggcagca gaacatcgac
    26101 gctacctgtt ggggtttgca gggtaaattc cgtaccgtgc gaggttacat ggatattggt
    26161 ggcggtgaaa tcgctgttgg cggcattagg tgagaaacgc cacactttgc gtgagccaat
    26221 tacgctctgc cagttcaacc agtcgttgtt gtcggcgttc ataatggcga taccgttttc
    26281 cggcaggccg ctaaagattt cacctttcgc tttcgcgaca ccagcaagcg agccaaaacc
    26341 ttccagatgc gccgctgcca ggttgttgac cagcgccgct tccgggcgag tcagactcac
    26401 agtccaggca atttcaccct gctggttcgc gccaagttca ataactgcgt aatcgtattc
    26461 cggcgttaag cgcaacagcg tcatcggtac accgatgtcg ttattgagat tgcctgccgt
    26521 ataaagtgtg ttgccgcact ggcttaaaat tgccgccgtc atctctttaa cggaggtttt
    26581 gccggaggaa cctgtcagag caaccacgcg cgccggaact tgctggcgaa cccatgcagc
    26641 cagttcacca aacgccagac gcgtatcctt gacgattaac tgcggcagat caatatccag
    26701 cggacggcta accagtagtg cgcctgcgcc gccagctttc gcttggtcgg caaaatcatg
    26761 agcatcgaaa cgttcgcctt tcagggcaac aaacaggcag cccggcgtca gttttcgcgt
    26821 gtcagtggtt acagcatcaa gggtaatatc tgcaccttgc agttcaccgt tgagaatatc
    26881 ggtaagttgg ctaagggtta cgctaatcat gcaatcaccc ccagcagacg cgccaccgtg
    26941 acgcgatcgg agtagtccag acgctgattg ccaacaatct ggtaatcctc atggcctttg
    27001 cccgcaacca gtaccacatc attctcttta gcctgcataa cggcgcaagt caccgcttca
    27061 gcacggcctt ccatcacttt ggcatatccg gcatctaaca ttcccgccag aatatcgttg
    27121 atgatggcac gaggctcttc ggtacgcggg ttatcgtccg tcaccaccgc cacgtcagca
    27181 aactcttcag caattgcgcc catcagtgga cgtttgcctt tatcacgatc gccaccgcag
    27241 ccaaagacgc accacagctt gcccgcacag tgcaggcgcg ccgcctgtaa ggctttttcc
    27301 agtgcatccg gcgtatgcgc gtaatccacc accaccgtcg gtttgcctgg tgcagtgaac
    27361 acttccatac gtccgcaaac cggttgcaga cgcgcggcgg ttttcagcaa atcagccagc
    27421 ggatagccga gtgccagcag tgtcgccagc gccagcagca ggttgctgac gttgaatgcg
    27481 cccatcaggc gactttcaat ttccccatcg ccccaactcg agctaaagcg aatggtcgca
    27541 ccgctgtcgt gatagttcac gtcggtcgct ttcaaccagc gtccgtgaca gttcggattg
    27601 atatgatctt ccattgatac cgcaaccgcg tccggcagtt ttgccagcca gcggcggccc
    27661 acttcatcgt cggcgttaat aatcgcctga ccgcaatgat gtgcagaata aagcagccat
    27721 ttcgcggctt cgtagtgttc catatcacca tgataatcaa ggtgatcgcg gcttaagttg
    27781 gtaaagaccg acgccgcaaa tttcaatgcc gccacacggt gttgtaccag cccgtgggat
    27841 gaaacttcca ttgcgcaaaa agtcgcgccc tgatccacca gccccgccag ctcatgctga
    27901 acatcgactg ccgaaccggt tgtattttct gtcgggatca ctttccccag caggccgtta
    27961 ccaacggtgc ccattaccgc gctggtttcg ccaagcagtt ggctccactg cgccaacagc
    28021 tgggtagtcg tggttttgcc gttggtgccc gttacgccca cgagacgtaa attgtcagag
    28081 ggttcatggt aaaagcggcc cgccagtgca gataaacgct cgttgagctg gctgagatag
    28141 atgaccggta cgccgtgcat ttcacggatt tcaccgtcgg tcgcctcatc tttcgcctct
    28201 gcaataatgg cagccacacc ttgcgctatc gcctgcggga tatatcgacg cccgtccgcc
    28261 tgatgaccta ctacagctac aaagagatcg cccgccgcag ccacacggct gtcgagtgtc
    28321 atctctcgca gtgctcgcga aggtgcgtct ggcacccacg gagcaagaag gtcgcgcaaa
    28381 ttacgatctg ccacctgtac cctcgccttg attaatcaca aattcatttt tatcgcccgt
    28441 tgtcagcgca tccggctcga tgttcatggt acgcaatacg ccgcccatga tggcaccaaa
    28501 gaccggcgcg gaaacggcgc cgccgtagta tttacccgcc tgcggatcgt tgataacaac
    28561 aaccagcgcg aagcgcggct gactcgcagg cgcaacgcct gcggtataag caatatattt
    28621 attgatgtag cgaccgtccg gcccgacctt tttcgcggta ccggttttaa tggcgatacg
    28681 atagccttta atcgccgcct tcacgccgcc gccgcccggt agcgccacgc tttccatcat
    28741 atgcaccacg gtgcgaacaa tggattccgg gaagacacgt tcaccgggaa ccgggggatc
    28801 aactttggta atcgacagtg ggcgataaat gccgtagctg ccgatagttg cgtagactcg
    28861 cgctaactgt aacggtgtta ccattagccc gtagccgaaa gagaaggtgg ccctctctat
    28921 gtcagaccac cgttgttttt gaggatataa gccactgcgt tctccgacca accccaaatt
    28981 ggtcgctttt cccagtccaa aacgtgagta agtatctact aacgctgagg acggcatcgc
    29041 taacgccagt ttggaaacac cgacgttact cgacttctgt aataccccgg tcagggttaa
    29101 ttcgctgtag cgcgccacgt ctttgatttc gtggccgtta attcgataag gaacggtatt
    29161 gagtaccgag ttttcccgca ccacgccacg ttgcaacgcg gtcatcacca ccatcggttt
    29221 aaccgttgag cccggttcaa acacgtcggt gatggtacgg ttacgcatcg cttctttcgg
    29281 cgtgccgctc agattatttg ggttgtatga cgggctgtta gccatcgcca gcacttcacc
    29341 ggtgttgaca tccaccagca cggcgctacc agattcagcc ttgttaaagg ccactgcgtt
    29401 gttcagttcg cgataaacca gcgcctgcag gcgttcatca atactcagcg tcaggttgtg
    29461 cgctgcctgg ctgtcagtag aagaaatatc ttcaattacg cgaccatagc ggtctttacg
    29521 cacaatgcgc tcacccggct gcccggtaag ccatttatcg aaactcttct cgacaccctc
    29581 aatcccttgg ctatcgacgt tagtaaaacc gatgaggtga gcagtcactt cgccggatgg
    29641 ataataacgg cgagattctt cacgcagatg aatccccggc agtttcagtt ttttgatgta
    29701 gtccgccatg tcagggttta cctgacgcgc cagataaata aagcgccctt tcgggttggc
    29761 gttaatgcgg gctgagagct gatccagcgg aatattgagt gcgttagcca gcgccttcca
    29821 gcgatcaccg acgctgatac cgccagcgtc atgcacttct ttcgggtcag cccaaatcgc
    29881 tttaaccggc acgctcaccg ctaacgggcg accagaacgg tcagtaatca tgccgcggga
    29941 ggtggaaact tgctgaacgc gaagagaacg catgtcgccc tctttcacca gcatatccgg
    30001 ggagataact tgtaaccacg ctacgcgtcc gagcagaaaa gccagcgcca ggagaataca
    30061 gccgcataac aacgcaaaac gccaactgat aaagttggca tgttcttcct gacgttttgg
    30121 tttctgcgtt ttcgccgctg ctttcatgcg tcgcgtttat ccttattttt gcactacgat
    30181 attttcttgt gacggatcaa catgctgcat ttgcagcttt tccgtggcta tcctttccac
    30241 ccggctatgg tcgccgagcg cattctcttc aaggatcagg ttgcgccatt caatgtctaa
    30301 agcatctcgc tccagcacca gttgttcgcg ctgagcggtc agtaaacggg tatggtgcgc
    30361 cgtggttacc acggtcaccg ccgtcaaaat aatgcaaatg aacaggcaga gtggcagctt
    30421 cccaaatcgc aaaagatcgt caccgataac gccaggcaag gcatggcgct cgtggcttcc
    30481 catcgatcct ttaactttgc ttagagcttc tgtcactctg ctgatcatgc gttcgtcctc
    30541 tctgcaatac gcagaactga actacgggca cgagggttct cagccacctc ttcttcgccc
    30601 ggcatcaact tacctagtgc tcgcaactga cggcccccca gttttttgag ctgctcttct
    30661 gtcatcggta accctgccgg aacttgcgga ccgcggctgt tttcacgcat aaaacgtttc
    30721 acaatgcggt cttcaagtga gtggaagctg atgatcgaaa gccgcccacc cggggccagc
    30781 acattgagcg agctttttag cgcctgctct atctcctcca gttcactgtt tacccaaatg
    30841 cgcaccgcct ggaaggtacg ggtcgcggga tgtttaaact tatctttcac cggcgttgcg
    30901 gcagccacga cttccgccag ttctttggtg cgggtcatcg gctgttcgcg gttacgctcg
    30961 acaatggcgc gggcaatgcg tttggcaaaa cgctcttcac cataggtttt caatacccag
    31021 gcgatatcgg cttcttctgc ggtttgtagc cattcagcgg ctgactgccc acgggttggg
    31081 tccatacgca tgtccagcgg accatcgcgc ataaaggaaa agccgcgctc agcatcatca
    31141 agttgcggtg aagagacgcc aagatcgagg agaatgccgt cgatcttgcc gataagatcg
    31201 cgctcggcaa cgtattcgcc cagcgcggag aaaggtccgt ggatgatgga gaagcgcgga
    31261 tcatcaatag tcttcgcaac ggcgatagcc tgcgggtcgc gatcgatcgc cagcaaacgc
    31321 ccctcttcgc caagctgcga gaggatcaga cgtgagtgac caccgcgacc aaaagtccca
    31381 tcaatgtagg tgccatcagg acggatattg aggccattaa cggcttcatc cagcagcacc
    31441 gtagtatgtt tatagttttc catcatttta tagagacaag tcctgcagtc gctccgataa
    31501 gtctccggta gccaactgct ctgcgtcgat atcttccttg acctgttgat gccaggttgt
    31561 ttcatcccac agctcaaact tgttgaactg tccaaccagc atcacttctt ttgtcagccc
    31621 ggcatgttgc cgcagtactg gcgcgattaa caatcgacct gcgccatcca tctgacattc
    31681 gctggcatga cctaacagta ggcgctgcac acggcgctca accgggttca tgctcgacag
    31741 acgcgataat ttttgctcga taatttccca ttcaggcagg gggtaaagca gcaggcacgg
    31801 gtgatgaatg tcaatggtgc aaaccatttg accggcagcg ttctcaagca gctgttcccg
    31861 ataacgggta ggcactgata agcgcccttt gctgtcgaga ttgactaacg ttgctccccg
    31921 gaacatgcca gcctcacccc ttattcccac tttgccccac aatttcccac tgaaaggagt
    31981 ttacggagcg gaggaaaagc ttgtcaagcc agtcacaagg cataccgagg taaaaaaccc
    32041 gatgtttacg gctaatatca gcgttgttta ttttacgtcc aacgtgcgaa gcaaattaac
    32101 gttatgaata gctgtaagaa aaaatccctc atcgtcatca ccgttttaaa atgactcact
    32161 attttccaca gcaaatataa agtgtcagtt tgcgacgcga gcggcatttt aggacatatc
    32221 ttcccaggtt aacagtccct gttgcgtctg tgtggcgctt gatgccagcg ggtttgcgca
    32281 aaagcaaaga aaagtgtttg ttaaatcggc aattcatctc tgcatgtaac aaaataatgc
    32341 aaaaacgcgc tatcgatatc acttaattat tgtgaatttc agctggtgag gtaattaacg
    32401 ggcaggttta aaagcattta tcaataatag ccaggaatag tcttatttac tttaaggata
    32461 ttgatgtcca ttcccgtact caacgcgcca gagagaaatt cacctggcgc gtatttttgt
    32521 tcgcggctta gctacggctg agcacgccgc ggcgatagag attacgttta atgcgcgtta
    32581 aaccaggttt tggcttacgc ggttcgtcca ggcttgccag gacaatctcc agcacacgct
    32641 ctgcgacatc gcggtgacgt tgagccactg ccagcaccgg gcactgtaag aagtcgagca
    32701 gttcgttatc gccaaaggtg gcaattgcca ggtcagaagg cagtttgccg tcacgacgta
    32761 gcgtgacatc catcacccct tgcaacaacg caaacgacgt tgtgaacagt gcctgcggca
    32821 tcggatgcgt ttccagccat ttttcgaata actgggcagc tgcctcccgc tcatagctgt
    32881 tggcataaag gaaatgcact tcgcgaggat catctttcca ggcagtacgg aaaccttgtt
    32941 cacgcaggaa gctgacagaa agctccggca gcgcgccaag ataaagtacc gtctcggcgg
    33001 gaaacttacg taactcttcc gccagcattt cggcatcatc ctgatcggca ccaaccacgc
    33061 tggtgaagtg ttcacgatcg agggcgcggt ccagcgcgac aatcgggaac gggtcgttag
    33121 cccagcgttg ataaaaagga tgctcaggtg gcaacgacgt cgaaacaata atggcatcaa
    33181 cctgacgctg taaaaggtgc tcaatgcagc gcatttcgtt gtcgggctga tcttccgagc
    33241 aggcaatcag cagttgataa ccccgttgcc gcgcctggcg ttcaagatag ttagcgatgc
    33301 gggtatagct ggtgttctcc agatcgggga tcacaagacc aatagaacgt gtgcgtccag
    33361 cacgaagccc agctgccacg gcgttcgggt ggtaattgtg ctcacgcacc acagccatga
    33421 ctttttcaac ggttttgtcg ctcacacggt attgcttcgc tttgccgtta ataacatagc
    33481 ttgcagtggt ccgcgacact cccgccagcc gagcgatttc atccagtttc acaattgccc
    33541 cttgcgtaaa atgtaaaaac catagccatt gtactggtat gggttaaatt cattaacatc
    33601 taagcgcaga aaattaactg cggcaactgc ttttattcca ggttcccact gatttcgcaa
    33661 aaaaagccca acgtgacatg ttgggctgca aattgcgcat tgagatcatt ctcaacgcat
    33721 tattttatcg ccgcgtgaaa gtccgaccac accagagcga gcaacttcca caattttcgc
    33781 cacatcgcga atcgatgcta aaaatgcatc aagcttatcg ctggtgcctg ctaattgaac
    33841 ggtataaagc gagggtgtga catcgataat ttgcccacgg aatatttccg tattacgttt
    33901 cacttcgtca cgcccgtaac cgctggcctg aattttcacc agcatgattt cccgctcaac
    33961 gtgcgcaccc tgccccaact cgctcacgcg caagacatcg accagcttgt gtaattgctt
    34021 ttcgatttgc tcaagcactt tttcatcgcc cacggtctga atggtcatac gcgataatgt
    34081 cggatcgtcg gttggcgcaa cggtcaggct ttcaatgttg tagccacgct gggaaaaaag
    34141 gccaatcacg cgggataacg cgcctgattc attttcgagt aagactgata atatccggcg
    34201 cataatcagg ttctctccgt tttgcttaac cacatttcat ccattccgcc cccgcgaatc
    34261 tgcatcgggt agacgtgctc actgccatcg acggtaacat caacaaacac cagacgatta
    34321 ttgcgcacct gttccagcgc ctcgctaagt ttgctttcca gttcttgcgg atgagagatc
    34381 tggatcccga cgtgaccata ggcttccgcc agacggacga agtctggtaa ggactgcata
    34441 taagattgtg aatgacggcc ggaatagatc atgtcctgcc actgcttcac catccccaga
    34501 tagcggttat tgagattcac caccagtacg ggcaactcat attgcaacgc ggtagacagt
    34561 tcctggatgt tcatctgaat actgccgtca ccagtgacgc aaaccacggt ttcttctggc
    34621 aacgccattt tgacgcccag tgccgcaggt aaaccaaaac ccatcgtgcc gaggccgcca
    34681 gagttgatcc agcgacgcgg tttgtcgaat ggataataaa gcgcagcaaa catctggtgc
    34741 tgcccgacat cggacgtcac gtaagcgtcc ccattagtca accgccaaag agtctcgatc
    34801 accgcctgcg gtttaatctt ttcactgtga gtgtcatatt tcaggcactg acgagcgcgc
    34861 cactgttcaa tctgctgcca ccagtcgcgg atctcatcca gcggttgatg ggcggattct
    34921 tgcgacagga gttcaagcat ttgttcgagg acctggcgag catcccccac aatcgggata
    34981 tcagcagtca cggttttaga aattgaggtt ggatcaatat cgatatgcag aacagtggca
    35041 tttgggcagt actttgccag attgttcgtc gttcggtcat caaaacgtac cccgacggcg
    35101 aaaatcacat ccgcgttatg catcgtcata ttggcttcgt aggtaccgtg cattcccaac
    35161 atgcccagtg cctggcgatg cgttgccgga aacgccccca gccccatcaa agaggaaaca
    35221 acgggcagat tcaacacctc caccgcttct ttcaactgca gatggcagcc cgccgtgatt
    35281 gccccaccgc ctacgtagac aaccggtttt tttgccgcca ccagcgtttg cagagcgcgc
    35341 ttaatttgcc ctttatgccc ggaggtcgtg ggattgtaag aacgcatact gaccgactcc
    35401 ggccagacat agggtaattt gttcgccgga tttaggatat ctttcggtaa atcaacgacc
    35461 acgggtccag gacgaccact tgccgctagc cagaaagcct ttttcagcac ctgcggaatg
    35521 tcttccgttt gcttaaccag aaaactgtgt ttaaccaccg ggcgagaaat ccccaccatg
    35581 tcgcactcct gaaaggcatc ataacctatc aacgaggtcg ctacctgccc ggaaaggaca
    35641 actaatggaa tggaatccat ataagcggtg gcaatgccag taattgcatt ggtcgcccct
    35701 ggacccgacg ttaccagcac gacgccgact tccccggtcg cgcgcgccag accatcggcc
    35761 atatgcaccg ccgcctgctc atggcgaact aaaacatgat cgataccacc cacggtatgc
    35821 aatgcatcgt aaatatcaag gaccgcgcct ccaggataac cgaatacttg tttaacgccc
    35881 tgatcgataa gcgatcggac gaccatctcg gctccagaca acatctccat ggcctgcctc
    35941 actgtttgac ggaaaaaatg tgtaaaacac attaaccgtc cgatcttgct taagcaattg
    36001 gcagacagag acataaacac ggggtaataa gaataaacag gaggaaaggt gaaaagaaac
    36061 gataaaatcc
//
LOCUS       DADSYM010000027        35952 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2415
            SAMN08039579-rid11723173.denovo.027, whole genome shotgun sequence.
ACCESSION   DADSYM010000027 DADSYM010000000
VERSION     DADSYM010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039579
            Sequence Read Archive: SRR6362533
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 35952)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35952)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980162.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 03:39:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,484
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 4,347
            CDSs (with protein)               :: 4,347
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 43 of 85
            Pseudo Genes (incomplete)         :: 37 of 85
            Pseudo Genes (internal stop)      :: 19 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35952
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039579-rid11723173.denovo.027"
                     /strain="LGE2415"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8302 N 95.9628 W"
                     /collection_date="2015-08-24"
     gene            complement(152..1522)
                     /locus_tag="J1Y83_001136"
     CDS             complement(152..1522)
                     /locus_tag="J1Y83_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001000969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBA7629304.1"
                     /translation="MNDYKMTPGERRATWGLGTVFSLRMLGMFMVLPVLTTYGMALQG
                     ASEALIGIAIGIYGLTQAVFQIPFGLLSDRIGRKPLIVGGLAVFAAGSVIAALSDSIW
                     GIILGRALQGSGAIAAAVMALLSDLTREQNRTKAMAFIGVSFGITFAIAMVLGPIITH
                     KLGLHALFWMIAILATTGIALTIWVVPNSSTHVLNRESGMVKGSFSKVLAEPRLLKLN
                     FGIMCLHILLMSTFVALPGQMADAGLPAAEHWKVYLATMLIAFGSVVPFIIYAEVKRK
                     MKQVFVFCVGLIVVAEIVLWNAQTQFWQLVVGVQLFFVAFNLMEALLPSLISKESPAG
                     YKGTAMGVYSTSQFLGVAIGGSLGGWIDGMFDGQGVFLAGAMLAAVWLAVASTMKEPP
                     YVSSLRIEIPDNVMADEKLEKSLLTTPGVKDVLLAKLEHSVYVKIDSKITNRFEIERV
                     INEVSY"
     gene            complement(1671..2561)
                     /gene="cyoE"
                     /locus_tag="J1Y83_001137"
     CDS             complement(1671..2561)
                     /gene="cyoE"
                     /locus_tag="J1Y83_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protoheme IX farnesyltransferase"
                     /protein_id="HBA7629305.1"
                     /translation="MIFKQYLQVTKPGIIFGNLISVIGGFLLASKGSIDYPLFIYTLV
                     GVSLVVASGCVFNNYIDRDIDRKMERTKNRVLVKGLISPAVSLVYATLLGIAGFMLLW
                     FGANPLACWLGVMGFVVYVGVYSLYMKRHSVYGTLIGSLSGAAPPVIGYCAVTGEFDS
                     GAAILLAIFSLWQMPHSYAIAIFRFKDYQAANIPVLPVVKGISVAKNHITLYIIAFAV
                     ATLMLSLGGYAGYKYLVVAAAVSVWWLGMALRGYKVADDRIWARKLFGFSIIAITALS
                     VMMSVDFMVPDSHTLLAAVW"
     gene            complement(2573..2902)
                     /locus_tag="J1Y83_001138"
     CDS             complement(2573..2902)
                     /locus_tag="J1Y83_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit IV"
                     /protein_id="HBA7629306.1"
                     /translation="MSHSNVSGGASHGSVKTYMTGFILSIILTVIPFWMVMTGAASPA
                     VILGTILAMAVVQILVHLVCFLHMNTKSDEGWNMTAFVFTVLIIAILVVGSIWIMWNL
                     NYNMMMH"
     gene            complement(2902..3516)
                     /locus_tag="J1Y83_001139"
     CDS             complement(2902..3516)
                     /locus_tag="J1Y83_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit III"
                     /protein_id="HBA7629307.1"
                     /translation="MATDTLTHATAHAHEHGHHDAGGTKIFGFWIYLMSDCILFSILF
                     ATYAVLVNGTAGGPTGKDIFELPFVLVETFLLLFSSITYGMAAIAMYKNNKSQVISWL
                     ALTWLFGAGFIGMEIYEFHHLIVNGMGPDRSGFLSAFFALVGTHGLHVTSGLIWMAVL
                     MVQIARRGLTSTNRTRIMCLSLFWHFLDVVWICVFTVVYLMGAM"
     gene            complement(3506..5497)
                     /gene="cyoB"
                     /locus_tag="J1Y83_001140"
     CDS             complement(3506..5497)
                     /gene="cyoB"
                     /locus_tag="J1Y83_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002444673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit I"
                     /protein_id="HBA7629308.1"
                     /translation="MFGKLSLDAVPFHEPIVMVTIAGIILGGLALVGLITYFGKWTYL
                     WKEWLTSVDHKRLGIMYIIVAIVMLLRGFADAIMMRSQQALASAGEAGFLPPHHYDQI
                     FTAHGVIMIFFVAMPFVIGLMNLVVPLQIGARDVAFPFLNNLSFWFTVVGVILVNVSL
                     GVGEFAQTGWLAYPPLSGIEYSPGVGVDYWIWSLQLSGIGTTLTGINFFVTILKMRAP
                     GMTMFKMPVFTWASLCANVLIIASFPILTVTVALLTLDRYLGTHFFTNDMGGNMMMYI
                     NLIWAWGHPEVYILILPVFGVFSEIAATFSRKRLFGYTSLVWATVCITVLSFIVWLHH
                     FFTMGAGANVNAFFGITTMIIAIPTGVKIFNWLFTMYQGRIVFHSAMLWTIGFIVTFS
                     VGGMTGVLLAVPGADFVLHNSLFLIAHFHNVIIGGVVFGCFAGMTYWWPKAFGFKLNE
                     TWGKRAFWFWIIGFFVAFMPLYALGFMGMTRRLSQQIDPQFHTMLMIAASGAVLIALG
                     ILCLVIQMYVSIRDRDQNRDLTGDPWGGRTLEWATSSPPPFYNFAVVPHVHERDAFWE
                     MKEKGEAYKKPDHYEEIHMPKNSGAGIVIAAFSTIFGFAMIWHIWWLAIVGFAGMIIT
                     WIVKSFDEDVDYYVPVAEIEKLENQHFDEITKAGLKNGN"
     gene            complement(5519..6475)
                     /gene="cyoA"
                     /locus_tag="J1Y83_001141"
     CDS             complement(5519..6475)
                     /gene="cyoA"
                     /locus_tag="J1Y83_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit II"
                     /protein_id="HBA7629309.1"
                     /translation="MRLRKYNKSLGWLSLFAGTVLLSGCNSALLDPKGQIGLEQRSLI
                     LTAFGLMLIVVIPAILMAVGFAWKYRASNKDAKYSPNWSHSNKVEAVVWTVPILIIIF
                     LAVLTWKTTHALEPSKPLAHDEKPITIEVVSMDWKWFFIYPEQGIATVNEIAFPANTP
                     VYFKVTSNSVMNSFFIPRLGSQIYAMAGMQTRLHLIANEPGTYDGISASYSGPGFSGM
                     KFKAIATPDRAAFDQWVAKAKQSPNTMSDMATFEKLAAPSEYNQVEYFSNVKPDLFAD
                     VINKFMAHGKSMDMTQPEGEHSAHEGMEGMEGMDMSHAESAH"
     gene            complement(6934..8409)
                     /gene="ampG"
                     /locus_tag="J1Y83_001142"
     CDS             complement(6934..8409)
                     /gene="ampG"
                     /locus_tag="J1Y83_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="muropeptide MFS transporter AmpG"
                     /protein_id="HBA7629310.1"
                     /translation="MSSQYLRIFQQPRSAILLILGFASGLPLALTSGTLQAWMTVENI
                     DLKTIGFFSLVGQAYVFKFLWSPLMDRYTPPFFGRRRGWLLATQILLLVAIAAMGFLE
                     PGTQLRWMAALAVVIAFCSASQDIVFDAWKTDVLPAEERGAGAAISVLGYRLGMLVSG
                     GLALWLADKWLGWQGMYWLMAALLIPCIIATLLAPEPTDTIPVPKTLEQAVVAPLRDF
                     FGRNNAWLILLLIVLYKLGDAFAMSLTTTFLIRGVGFDAGEVGVVNKTLGLLATIVGA
                     LYGGILMQRLSLFRALLIFGILQGASNAGYWLLSITDKHLYSMGAAVFFENLCGGMGT
                     SAFVALLMTLCNKSFSATQFALLSALSAVGRVYVGPVAGWFVEAHGWSTFYLFSVAAA
                     VPGLILLLVCRQTLEYTRVNDNFISRTEYPAGYAFAMWTLAAGISLLAVWLLLLTMDA
                     LDLTHFSFLPALLEVGVLVALSGVVLGGLLDYLALRKTHLT"
     gene            complement(8453..9031)
                     /locus_tag="J1Y83_001143"
     CDS             complement(8453..9031)
                     /locus_tag="J1Y83_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414968.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HBA7629311.1"
                     /translation="MFKKILFPLVALFMLAGCAKPPTTIEVSPTITLPQQDPSLMGVT
                     VSINGADQRTDQALAKVTRDNQIVTLTASRDLRFLLQEVLEKQMTARGYMVGPNGPVN
                     LQIIVSQLYADVSQGNVRYNIATKADIAIIATAQNGNKMTKNYRASYNVEGAFQASNK
                     NIADAVNSVLTDTIADMSQDTSIHEFIKQNAR"
     gene            9336..9653
                     /gene="bolA"
                     /locus_tag="J1Y83_001144"
     CDS             9336..9653
                     /gene="bolA"
                     /locus_tag="J1Y83_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414969.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator BolA"
                     /protein_id="HBA7629312.1"
                     /translation="MMIRERIEEKLRAAFQPVFLEVVDESYRHNVPAGSESHFKVVLV
                     SDRFTGERFLNRHRMIYSTLAEELSTTVHALALHTYTIKEWEGLQDTVFASPPCRGAG
                     SIA"
     gene            9997..11295
                     /gene="tig"
                     /locus_tag="J1Y83_001145"
     CDS             9997..11295
                     /gene="tig"
                     /locus_tag="J1Y83_001145"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001716227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HBA7629313.1"
                     /translation="MQVSVETTQGLGRRVTITIAADSIETAVKSELVNVAKKVRIDGF
                     RKGKVPMNIVAQRYGASVRQDVLGDLMSRNFIDAIIKEKINPAGAPTYVPGEYKLGED
                     FTYSVEFEVYPEVELQGLEAIEVEKPIVEVTDADVDGMLDTLRKQQATWKEKDGAVEA
                     EDRVTIDFTGSVDGEEFEGGKASDFVLAMGQGRMIPGFEDGIKGHKAGEEFTIDVTFP
                     EEYHAENLKGKAAKFAINLKKVEERELPELTAEFIKRFGVEDGSVEGLRAEVRKNMER
                     ELKSAIRNRVKSQAIEGLVKANDIDVPAALIDSEIDVLRRQAAQRFGGNEKQALELPR
                     ELFEEQAKRRVVVGLLLGEVIRTNELKADEERVKGLIEEMASAYEDPKEVIEFYSKNK
                     ELMDNMRNVALEEQAVEAVLAKAKVTEKETTFNELMNQQA"
     gene            11541..12164
                     /gene="clpP"
                     /locus_tag="J1Y83_001146"
     CDS             11541..12164
                     /gene="clpP"
                     /locus_tag="J1Y83_001146"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HBA7629314.1"
                     /translation="MSYSGERDNFAPHMALVPMVIEQTSRGERSFDIYSRLLKERVIF
                     LTGQVEDHMANLIVAQMLFLEAENPEKDIYLYINSPGGVITAGMSIYDTMQFIKPDVS
                     TICMGQAASMGAFLLTAGAKGKRFCLPNSRVMIHQPLGGYQGQATDIEIHAREILKVK
                     GRMNELMALHTGQSLEQIERDTERDRFLSAPEAVEYGLVDSILTHRN"
     gene            12290..13564
                     /gene="clpX"
                     /locus_tag="J1Y83_001147"
     CDS             12290..13564
                     /gene="clpX"
                     /locus_tag="J1Y83_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001396736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATP-binding subunit ClpX"
                     /protein_id="HBA7629315.1"
                     /translation="MTDKRKDGSGKLLYCSFCGKSQHEVRKLIAGPSVYICDECVDLC
                     NDIIREEIKEVAPHRERSALPTPHEIRNHLDDYVIGQEQAKKVLAVAVYNHYKRLRNG
                     DTSNGVELGKSNILLIGPTGSGKTLLAETLARLLDVPFTMADATTLTEAGYVGEDVEN
                     IIQKLLQKCDYDVQKAQRGIVYIDEIDKISRKSDNPSITRDVSGEGVQQALLKLIEGT
                     VAAVPPQGGRKHPQQEFLQVDTSKILFICGGAFAGLDKVISHRVETGSGIGFGATVKA
                     KSDKASEGELLAQVEPEDLIKFGLIPEFIGRLPVVATLNELSEEALIQILKEPKNALT
                     KQYQALFNLEGVDLEFRDEALDAIAKKAMARKTGARGLRSIVEAALLDTMYDLPSMED
                     VEKVVIDESVIDGQSKPLLIYGKPEAQQASGE"
     gene            13752..16106
                     /gene="lon"
                     /locus_tag="J1Y83_001148"
     CDS             13752..16106
                     /gene="lon"
                     /locus_tag="J1Y83_001148"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001469947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HBA7629316.1"
                     /translation="MNPERSERIEIPVLPLRDVVVYPHMVIPLFVGREKSIRCLEAAM
                     DHDKKIMLVAQKEASTDEPGVNDLFTVGTVASILQMLKLPDGTVKVLVEGLQRARISA
                     LSDNGEHFSAKAEYLESPTIDEREQEVLVRTAISQFEGYIKLNKKIPPEVLTSLNSID
                     DPARLADTIAAHMPLKLADKQSVLEMSDVNERLEYLMAMMESEIDLLQVEKRIRNRVK
                     KQMEKSQREYYLNEQMKAIQKELGEMDDAPDENEALKRKIDAAKMPKEAKEKAEAELQ
                     KLKMMSPMSAEATVVRGYIDWMVQVPWNARSKVKKDLRQAQEILDTDHYGLERVKDRI
                     LEYLAVQSRVNKIKGPILCLVGPPGVGKTSLGQSIAKATGRKYVRMALGGVRDEAEIR
                     GHRRTYIGSMPGKLIQKMAKVGVKNPLFLLDEIDKMSSDMRGDPASALLEVLDPEQNV
                     AFSDHYLEVDYDLSDVMFVATSNSMNIPAPLLDRMEVIRLSGYTEDEKLNIAKRHLLP
                     KQIERNALKKGELTVDDSAIIGIIRYYTREAGVRGLEREISKLCRKAVKQLLLDKSLK
                     HIEINGDNLHDYLGVQRFDYGRADNENRVGQVTGLAWTEVGGDLLTIETACVPGKGKL
                     TYTGSLGEVMQESIQAALTVVRARAEKLGINPDFYEKRDIHVHVPEGATPKDGPSAGI
                     AMCTALVSCLTGNPVRADVAMTGEITLRGQVLPIGGLKEKLLAAHRGGIKTVLIPFEN
                     KRDLEEIPDNVIADLDIHPVKRIEEVLTLALQNEPSGMQVLTAK"
     gene            16315..16587
                     /gene="hupB"
                     /locus_tag="J1Y83_001149"
     CDS             16315..16587
                     /gene="hupB"
                     /locus_tag="J1Y83_001149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016535194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein HU-beta"
                     /protein_id="HBA7629317.1"
                     /translation="MNKSQLIDKIAAGADISKAAAGRALDAIIASVTESLKEGDDVAL
                     VGFGTFAVKERAARTGRNPQTGKEITIAAAKVPSFRAGKALKDAVN"
     gene            16779..18650
                     /gene="ppiD"
                     /locus_tag="J1Y83_001150"
     CDS             16779..18650
                     /gene="ppiD"
                     /locus_tag="J1Y83_001150"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HBA7629318.1"
                     /translation="MMDSLRTAANSLVLKIIFGIIIVSFILTGVSGYLIGGGNNYAAK
                     VNDQEISRGQFENAFNSERNRMQQQLGDQYSELAANEGYMKTLRQQVLNRLIDEALLD
                     QYARELKLGISDEQVKQAIFATPAFQVDGKFDNSRYNGILNQMGMTADQYAQALRNQL
                     TTQQLINGVAGTDFMLKGETDELAALVAQQRVVREATIDVNVLAAKQPVTEQEIASYY
                     EQNKNNFMTPEQFRVSYIKLDAATMQQPVSDADIQSYYDQHQDQFTQPQRTRYSIIQT
                     KTEDEAKAVLDELNKGGDFAALAKEKSADIISARNGGDMGWLEDATIPDELKNAGLKE
                     KGQLSGVIKSSVGFLIVRLDDIQPAKVKSLDEVRDDIAAKVKHEKALDAYYALQQKVS
                     DAASNDTESLAGAEQAAGVKATQTGWFSKDNLPEELNFKPVADAIFNGGLVGENGAPG
                     INSDIITVDGDRAFVLRISEHKPEAVKPLADVQEQVKALVQHNKAEQQAKVDAEKLLV
                     DLKAGKGAEAMQAAGLKFGEPKTLSRSGRDPISQAAFALPLPAKDKPSYGMATDMQGN
                     VVLLALDEVKQGSMPEDQKKAMVQGITQNNAQIVFEALMSNLRKEAKIKIGDALEQQ"
     gene            18801..19169
                     /locus_tag="J1Y83_001151"
     CDS             18801..19169
                     /locus_tag="J1Y83_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA7629319.1"
                     /translation="MKHGIKALLITLSLACAGMSHSALAAASVAKPTTVETKAEAPAA
                     QSKAVPAKASDEEGTRVSINNASAEELARAMNGVGLKKAQAIVSYREEYGPFKTVEDL
                     KQVPGMGNSLVERNLAVLTL"
     gene            19275..19673
                     /locus_tag="J1Y83_001152"
     CDS             19275..19673
                     /locus_tag="J1Y83_001152"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbgC/FadM family acyl-CoA thioesterase"
                     /protein_id="HBA7629320.1"
                     /translation="MQTQIKVRGYHLDVYQHVNNARYLEFLEEARWDGLENSDSFQWM
                     TAHNIAFVVVNININYRRPAVLSDLLTITSQLQQLNGKSGILSQVITLEPEGQVVADA
                     LITFVCIDLKTQKALALEGELREKLEQMVK"
     gene            complement(19725..20420)
                     /gene="queC"
                     /locus_tag="J1Y83_001153"
     CDS             complement(19725..20420)
                     /gene="queC"
                     /locus_tag="J1Y83_001153"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001398710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HBA7629321.1"
                     /translation="MKRAVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRHRAEID
                     VARELALKLGARAHKVLDVTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNIL
                     FLTLAAIYAYQVKAEAVITGVCETDFSGYPDCRDEFVKALNHAVSLGMAKDIRFETPL
                     MWIDKAETWALADYYGKLDLVRNETLTCYNGIKGDGCGHCAACNLRANGLNHYLADKP
                     TVMAAMKQKTGLN"
     gene            complement(20485..22185)
                     /locus_tag="J1Y83_001154"
     CDS             complement(20485..22185)
                     /locus_tag="J1Y83_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SgrR family transcriptional regulator"
                     /protein_id="HBA7629322.1"
                     /translation="MRLLNRLNQYQRLWQPSAGKPQTVTVSELAERCFCSERHVRTLL
                     RQAQEAGWLEWQAQSGRGKRGQLRFLVTPESLRNAMMEQALETGKQQDVLELAQLAPG
                     ELRTLLQPFMGGQWQNDTPTLRIPYYRPLEPLQPGFLPGRAEQHLAGQIFSGLTRFDN
                     NTQRPIGDLAHHWETSTDGLRWDFYLRSTLHWHNGDAVKASHLHQRLLMLLQLPALDQ
                     LFISVKRIEVTHPQCLTFFLHRPDYWLAHRLASYCSHLAHPQFPLIGTGPFRLTQFTA
                     ELVRLESHDYYHLRHPLLKAVEYWITPPLFEKDLGTSCRHPVQITIGKPEELQRVSQV
                     SSGISLGFCYLTLRKSPRLSLWQARKVISIIHQSGLLQTLEVGENLITASHALLPDWT
                     IPQWQVPDEVKLPKTLTLVYHLPIELHTMAERLQATLAAEGCELTIIFHNAKNWDDTT
                     LLAHADLMMGDRLIGEAPEYTLEQWLRCDPLWPHVFDAPAYAHLQSTLDAVQVMPDEE
                     NRFNALKAVFSQLMADATLTPLFNYHYRISAPPGVNGVRLTPRGWFEFTEAWLPAPSQ
                     "
     gene            22285..23103
                     /gene="cof"
                     /locus_tag="J1Y83_001155"
     CDS             22285..23103
                     /gene="cof"
                     /locus_tag="J1Y83_001155"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HMP-PP phosphatase"
                     /protein_id="HBA7629323.1"
                     /translation="MARLAAFDMDGTLLMPDHHLGEKTLSTLARLRERDITLTFATGR
                     HALEMQHILGALSLDAYLITGNGTRVHSLEGELLHRDDLPADVAELVLYQQWDTRASM
                     HIFNDDGWFTGKEIPALLQAFVYSGFRYQIIDVKKMPLGSVTKICFCGDHDDLTRLQI
                     QLYEALGERAHLCFSATDCLEVLPVGCNKGAALTVLTQHLGLSLRDCMAFGDAMNDRE
                     MLGSVGSGFIMGNAMPQLRAELPHLPVIGHCRNQAVSHYLTHWLDYPHLPYSPE"
     gene            23256..23714
                     /locus_tag="J1Y83_001156"
     CDS             23256..23714
                     /locus_tag="J1Y83_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HBA7629324.1"
                     /translation="MLDKIDRKLLALLQQDCTLSLQALAEAVNLTTTPCWKRLKRLED
                     DGILIGKVALLDPEKIGLGLTAFVLIKTQHHSSEWYCRFVTVVTEMPEVLGFWRMAGE
                     YDYLMRVQVADMKRYDEFYKRLVNSVPGLSDVTSSFAMEQIKYTTSLPIE"
     gene            23744..25516
                     /locus_tag="J1Y83_001157"
     CDS             23744..25516
                     /locus_tag="J1Y83_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SmdA family multidrug ABC transporter
                     permease/ATP-binding protein"
                     /protein_id="HBA7629325.1"
                     /translation="MRLFAQLSWYFRREWRRYLGAVALLVIIAMLQLVPPKVVGIVVD
                     GVTEQHFTTGQILMWIATMVLIAVVVYLLRYVWRVLLFGASYQLAVELREDYYRQLSR
                     QHPEFYLRHRTGDLMARATNDVDRVVFAAGEGVLTLVDSLVMGCAVLIMMSTQISWQL
                     TLFALLPMPVMAIMIKRNGDALHERFKLAQAAFSSLNDRTQESLTSIRMIKAFGLEDR
                     QSALFAADAEDTGKKNMRVARIDARFDPTIYIAIGMANLLAIGGGSWMVVQGSLTLGQ
                     LTSFMMYLGLMIWPMLALAWMFNIVERGSAAYSRIRTMLAEAPVVIDGSDKVPEGRGE
                     LDVNIRQFTYPQTDHPALENVNFALKPGQMVGICGPTGSGKSTLLSLIQRHFDVSEGD
                     IRFHDIPLTKLQLDSWRSRLAVVSQTPFLFSDTVANNIALGCPNATQQEIEHVARLAS
                     VHDDILRLPQGYDTEVGERGVMLSGGQKQRISIARALLVNAEILILDDALSAVDGRTE
                     HQILHNLRQWGQGRTVIISAHRLSALTEASEIIVMQHGHIAQRGNHDELAQQSGWYRD
                     MYRYQQLEAALDDAPEIREEAIDA"
     gene            25509..27290
                     /locus_tag="J1Y83_001158"
     CDS             25509..27290
                     /locus_tag="J1Y83_001158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SmdB family multidrug efflux ABC transporter
                     permease/ATP-binding protein"
                     /protein_id="HBA7629326.1"
                     /translation="MRSFSQLWPTLKRLLAYGSPWRKPLGIAVLMMWVAAAAEVSGPL
                     LISYFIDNMVAKNNLPLKVVAGLAAAYVGLQLFAAGLHYAQSLLFNRAAVGVVQQLRT
                     DVMDAALRQPLSEFDTQPVGQVISRVTNDTEVIRDLYVTVVATVLRSAALVGAMLVAM
                     FSLDWRMALVAIMIFPVVLVVMVIYQRYSTPIVRRVRAYLADINDGFNEIINGMSVIQ
                     QFRQQARFGERMGEASRSHYMARMQTLRLDGFLLRPLLSLFSSLILCGLLMLFGFSAS
                     GTIEVGVLYAFISYLGRLNEPLIELTTQQAMLQQAVVAGERVFELMDGPRQQYGNDDR
                     PLQSGTIEVDNVSFAYRDDNMVLKNINLSVPSRNFVALVGHTGSGKSTLASLLMGYYP
                     LTEGEIRLDGRPLSSLSHSALRQGVAMVQQDPVVLADTFLANVTLGRDISEERVWQAL
                     ETVQLAELARSMSDGIYTPLGEQGNNLSVGQKQLLALARVLVETPQILILDEATASID
                     SGTEQAIQHALAAVREHTTLVVIAHRLSTIVDADTILVLHRGQAVEQGTHQQLLAAQG
                     RYWQMYQLQLAGEELAASVREEESLSA"
     gene            27471..27809
                     /gene="glnK"
                     /locus_tag="J1Y83_001159"
     CDS             27471..27809
                     /gene="glnK"
                     /locus_tag="J1Y83_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HBA7629327.1"
                     /translation="MKLVTVIIKPFKLEDVREALSSIGIQGLTVTEVKGFGRQKGHAE
                     LYRGAEYSVNFLPKVKIDVAIADDQLDEVIDIVSKAAYTGKIGDGKIFVAELQRVIRI
                     RTGEADEAAL"
     gene            27839..29125
                     /gene="amtB"
                     /locus_tag="J1Y83_001160"
     CDS             27839..29125
                     /gene="amtB"
                     /locus_tag="J1Y83_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter AmtB"
                     /protein_id="HBA7629328.1"
                     /translation="MKIATIKTGLASLAMLPGLVMAAPAVADKADNAFMMICTALVLF
                     MTIPGIALFYGGLIRGKNVLSMLTQVTVTFALVCILWVVYGYSLAFGEGNNFFGNINW
                     LMLKNIELTAVMGSIYQYIHVAFQGSFACITVGLIVGALAERIRFSAVLIFVVVWLTL
                     SYIPIAHMVWGGGLLASHGALDFAGGTVVHINAAIAGLVGAYLIGKRVGFGKEAFKPH
                     NLPMVFTGTAILYIGWFGFNAGSAGTANEIAALAFVNTVVATAAAILGWIFGEWALRG
                     KPSLLGACSGAIAGLVGVTPACGYIGVGGALIIGVVAGLAGLWGVTMLKRLLRVDDPC
                     DVFGVHGVCGIVGCIMTGIFAASSLGGVGFAEGVTMGHQLLVQLESIAITIVWSGVVA
                     FIGYKLADLTVGLRVPEEQEREGLDVNSHGENAYNA"
     gene            complement(29174..30034)
                     /gene="tesB"
                     /locus_tag="J1Y83_001161"
     CDS             complement(29174..30034)
                     /gene="tesB"
                     /locus_tag="J1Y83_001161"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase II"
                     /protein_id="HBA7629329.1"
                     /translation="MSQALKNLLTLLNLEKIEEGLFRGQSEDLGLRQVFGGQVVGQAL
                     YAAKETVPDERLVHSFHSYFLRPGDSKKPIIYDVETLRDGNSFSARRVSAIQNGKPIF
                     YMTASFQAPEAGFEHQKTMPSAPAPDGLPSETQIAQSLAHLLPPVLKDKFICDRPLEV
                     RPVEFHNPLKGHVAEPHRQVWIRANGSVPDDLRVHQYLLGYASDLNFLPVALQPHGIG
                     FLEPGIQIATIDHSMWFHRPFNLNEWLLYSVESTSASSARGFVRGEFYTQDGVLVAST
                     VQEGVMRNHN"
     gene            30252..30824
                     /locus_tag="J1Y83_001162"
     CDS             30252..30824
                     /locus_tag="J1Y83_001162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA7629330.1"
                     /translation="MKLVHMASGLAVAIALAACADKSADIQTPAPAANTSISATQQPA
                     IQQPNVSGTVWIRQKVALPPDAVLTVTLSDASLADAPSKVLAQKAVRTEGKQSPFSFV
                     LPFNPADVQPNARILLSAAITVNDKLVFITDTVQPVINQGGTKADLTLVPVQQTAVPV
                     QASGGATTTVPSTSPTQVNPSSAVPAPTQY"
     gene            complement(30855..31166)
                     /locus_tag="J1Y83_001163"
     CDS             complement(30855..31166)
                     /locus_tag="J1Y83_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MGMT family protein"
                     /protein_id="HBA7629331.1"
                     /translation="MEKEDSFPQRVWQIVAAIPEGYVTTYGDVAKLAGSPRAARQVGG
                     VLKRLPEGSTLPWHRVVNRHGTISLTGPDLQRQRQALLAEGVMVSGSGQIDLQLYRWN
                     Y"
     gene            31329..31425
                     /gene="ffs"
                     /locus_tag="J1Y83_001164"
     ncRNA           31329..31425
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="J1Y83_001164"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            31545..31898
                     /locus_tag="J1Y83_001165"
     CDS             31545..31898
                     /locus_tag="J1Y83_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1428 domain-containing protein"
                     /protein_id="HBA7629332.1"
                     /translation="MKYVDGFVVAVPADKKDAYREMAAKAAPLFKEFGALRIVECWAS
                     DVPDGKVTDFRMAVKAEENEEVVFSWIEYPSKEVRDAANQKMMSDPRMKEFGESMPFD
                     GKRMIYGGFESIIDE"
     gene            complement(31940..33490)
                     /gene="pdeB"
                     /locus_tag="J1Y83_001166"
     CDS             complement(31940..33490)
                     /gene="pdeB"
                     /locus_tag="J1Y83_001166"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414990.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase
                     PdeB"
                     /protein_id="HBA7629333.1"
                     /translation="MRTRHLVGLISGVLILSVLLPVGLSIWLAHQQVETSFIEELNTY
                     SSRVAIRANKVATQGKDALQELERWQGAACSEAHLMEMRRVSYSYRYIQEVVYIDNNV
                     PQCSSLEHESPPDTFPEPGKISKDGYRVWLTSHNDLGIIRYMVAMGTAHYVVMIDPAS
                     FIDVIPYSSWQIDAAIIGNAHNVVITSSDELAQGIITRLQKTPGEHIENNGIIYDILP
                     LPEMNISIITWASTKMLQKGWHRQVFIWLPLGLVIGLLAAMFVLRILRRIQSPHHRLQ
                     DAIENRDICVHYQPIVSLANGKIVGAEALARWPQTDGSWLSPDSFIPLAQQTGLSEPL
                     TLLIIRSVFEDMGDWLRQHPQQHISINLESTVLTSEKIPQLLREMINHYQVNPRQIAL
                     ELTEREFADPKTSAPIISRYREAGHEIYLDDFGTGYSSLSYLQDLDVDILKIDKSFVD
                     ALEYKNVTPHIIEMAKTLKLKMVAEGIETSKQEEWLRQHGVHYGQGWLYSKALPKEDF
                     LRWAEQHL"
     gene            complement(33654..34124)
                     /locus_tag="J1Y83_001167"
     CDS             complement(33654..34124)
                     /locus_tag="J1Y83_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308538.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA7629334.1"
                     /translation="MTEIQRLLTETIESLNTREKRDNKPRFSISFIRKHPGLFIGMYV
                     AFFATLAVMLQSETLSGSVWLLVVLFILLNGFFFFDVYPRYRYEDIDVLDFRVCYNGE
                     WYNTRFVPAALVEAILNSPRVADVHKEQLQKMIVRKGELSFYDIFTLARAESTS"
     gene            complement(34240..34791)
                     /gene="maa"
                     /locus_tag="J1Y83_001168"
     CDS             complement(34240..34791)
                     /gene="maa"
                     /locus_tag="J1Y83_001168"
                     /EC_number="2.3.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose O-acetyltransferase"
                     /protein_id="HBA7629335.1"
                     /translation="MSTEKEKMIAGELYHSADEALSRDRLRARRLIHRYNHSLAEEHT
                     LRQQILADLFGQVTEAYIEPTFRCDYGYNIFLGNNFFANFDCVMLDVCPIRIGDNCML
                     APGVHIYTATHPIDPVARNSGAELGKPVTIGNNVWIGGRAVINPGVTIGDNVVVASGA
                     VVTKDVPDNVVVGGNPARIIKKL"
     gene            complement(34964..35182)
                     /gene="hha"
                     /locus_tag="J1Y83_001169"
     CDS             complement(34964..35182)
                     /gene="hha"
                     /locus_tag="J1Y83_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin expression modulator Hha"
                     /protein_id="HBA7629336.1"
                     /translation="MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFY
                     SAADHRLAELTMNKLYDKIPSSVWKFIR"
     gene            complement(35208..35582)
                     /gene="tomB"
                     /locus_tag="J1Y83_001170"
     CDS             complement(35208..35582)
                     /gene="tomB"
                     /locus_tag="J1Y83_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001741190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hha toxicity modulator TomB"
                     /protein_id="HBA7629337.1"
                     /translation="MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAI
                     NLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYLDDTFMLFSSYGINMQDLQKWRK
                     SGNRLFRCFVNATKENPASLSC"
BASE COUNT         9223 a         8743 c         9528 g         8458 t
ORIGIN      
        1 atttatccct gattttcgga aaaagtataa tttacttctg atcgaatcgt ccagttttat
       61 ggaattacat gaagttttaa taatgtggga tttaaagaat aatgacgtga cgaagcacgg
      121 tgctgttcat gaatagcacc gtgcttaata tttaataaga tacttcgttg ataactcgct
      181 ctatttcgaa gcgattggtg attttactgt ctattttcac atagacgcta tgctctaact
      241 tcgccagcag tacatctttg acaccaggcg ttgtcagtaa gcttttttcc agtttttcat
      301 ctgccatcac gttgtccggg atttcaatgc gcaagctgct gacatacggc ggttctttca
      361 tggtactggc gactgccagc cacactgcgg ccagcattgc gccagcgaga aatacgccct
      421 gaccgtcaaa catgccgtca atccagccgc ccagcgaacc gccaatcgcc acgccaagaa
      481 actggctggt ggagtaaaca cccatcgccg tacctttgta acctgctggc gattctttac
      541 tgataagtga aggcagaagg gcttccatca aattaaacgc cacaaagaaa agctgcacgc
      601 cgaccaccag ttgccagaac tgcgtttgtg cgttccacaa cacaatttcc gcaaccacga
      661 tcaacccgac gcagaagaca aagacttgct tcattttgcg cttaacttca gcgtagataa
      721 tgaaaggcac gaccgagcca aaggcgatta acatcgttgc caggtagacc ttccagtgtt
      781 cagccgccgg taaccccgca tcagccatct gtccgggcag ggcaacaaag gtcgacatca
      841 gcaaaatatg cagacacata atgccaaagt tgagtttcag cagccgcggt tccgccagca
      901 ctttactgaa actgcctttc accattccgg actcacgatt aagtacgtga gtgctactgt
      961 tgggcacaac ccaaatggtc aacgcaatgc cggttgttgc cagaatagcg atcatccaga
     1021 acagcgcgtg cagcccaagt ttgtgagtga tgatcgggcc aagcaccatc gcaatggcaa
     1081 aggtaatgcc aaagctcacg ccgataaacg ccatcgcttt ggtgcggttt tgttcgcgcg
     1141 tgagatcgga aagcagcgcc ataacggcag cggcaatcgc accggagcct tgtagcgccc
     1201 ggcccagaat aattccccag atggagtcag agagcgcagc gataacgcta ccggcggcaa
     1261 acaccgccag cccaccgaca attaatggtt tgcgaccaat gcggtctgaa agcaggccaa
     1321 acggaatctg aaaaacggcc tgagtcagac cataaatacc aatggcaata ccgattaatg
     1381 cttcgctggc accttgcaga gccatgccgt acgtggtcag aaccggcaga accatgaaca
     1441 tgcccagcat gcgtaatgaa aatacggtcc ctaaacccca ggtcgcgcgc cgctcacctg
     1501 gcgtcatttt ataatcgttc attaccacct ctgttttaaa ttcgcgacta gtgtaaagcg
     1561 gcaagcgaat ggggtaaaca tgcggatttt ttagtaaata tttcgagagg gatatgattc
     1621 taatgctaag aaaaaaggtg ccgtagcacc tttttaactg agaggttgtg ttaccacaca
     1681 gcagccagca gcgtatgcga gtccggcacc ataaaatcaa cggacatcat caccgagagg
     1741 gcagtgatgg cgatgataga gaaaccgaac agcttgcgcg cccagattct gtcatcagca
     1801 actttatagc cgcgcagagc catgcctaac caccagacgc tcaccgcagc ggcgaccacc
     1861 agatatttat acccagcgta gccgccaaga gagagcatca gcgtggcaac ggcaaaggcg
     1921 atgatataca gcgtgatgtg attcttcgcc accgaaatgc ctttcaccac cggcagtacc
     1981 ggaatgtttg ccgcctgata atccttaaag cggaaaatgg ctatggcata agagtgaggc
     2041 atctgccaca ggctgaagat agccagcagg atcgctgcgc cgctatcgaa ctcgccggtt
     2101 accgcacagt aaccgatcac cggcggcgca gcgccggaga gcgaaccaat caatgtgccg
     2161 tagacagaat gacgtttcat gtacaggcta taaacgccga cataaaccac aaagcccatc
     2221 acccccagcc agcaggccag cggattcgcg ccaaaccaca gcagcataaa gccagcaata
     2281 cccagcaagg tggcgtacac cagcgagaca gcaggagaga tcaggccttt aaccagcacc
     2341 cgattcttcg tcctttccat ctttcggtcg atatccctgt cgatgtagtt gttaaacaca
     2401 caacccgacg ccacaaccag tgacacccca accagcgtgt agataaacag gggataatca
     2461 atgctgccct ttgaggccaa caggaatccc ccaatcaccg agatcaggtt gccaaagatg
     2521 atgcctggtt tcgttacttg caggtattgc ttaaatatca taaccgccgc tcttagtgca
     2581 tcatcatgtt gtagttgagg ttccacataa tccaaatgga gcccacaacc aggatggcga
     2641 tgattagcac ggtgaagaca aatgccgtca tattccagcc ttcatctgat ttggtattca
     2701 tgtgcaggaa gcacaccaga tgcaccagaa tctgtaccac tgccattgcc aggattgttc
     2761 ccagaattac ggccggagag gcagcccctg tcatcaccat ccagaacgga attaccgtca
     2821 ggattatcga caggataaag cctgtcatgt aggtctttac gctgccatgg gatgcgccgc
     2881 cgctcacgtt agaatgactc attacatcgc ccccatcaga taaacaacag tgaacacaca
     2941 gatccaaacc acatccagga agtgccagaa caggctcagg cacatgatgc gggtacggtt
     3001 agtgctggtc aggccgcgac gggcgatttg caccatcagc accgccatcc agataagacc
     3061 ggaagtgacg tgcagaccgt gcgtgccgac cagcgcaaag aatgctgaca ggaagccgct
     3121 gcgatccgga cccatgccgt taacaatcag gtgatggaat tcatagattt ccatcccgat
     3181 aaatccggca ccaaacaacc atgtcaacgc cagccaggag atcacctggc ttttgttgtt
     3241 tttgtacatg gcgatagccg ccatgccgta ggtgatggag ctgaacaaca gcaagaaagt
     3301 ttcaaccaga acgaacggca gttcgaaaat gtccttacct gtcggcccac ctgcggtgcc
     3361 gttcaccaga acggcatagg tagcaaacaa gatagagaac agaatgcagt cgctcatcag
     3421 gtagatccaa aatccgaaga ttttggttcc gcctgcatcg tggtgcccgt gttcgtgcgc
     3481 gtgggcagtc gcgtgcgtca aagtatcagt tgccattttt cagccctgcc ttagtaatct
     3541 catcgaaatg ctggttttcc agtttttcga tttctgccac cggcacgtag taatccacgt
     3601 cctcgtcgaa gcttttcacg atccaggtga tgatcatgcc tgcgaagcca acaatcgcca
     3661 gccaccagat atgccagatc atggcgaaac cgaagatggt ggagaaagcc gcaatgacga
     3721 taccggcacc gctgtttttc ggcatatgaa tttcttcata gtggtcaggc tttttgtacg
     3781 cttcgccttt ctctttcatt tcccagaatg catcacgttc gtgaacgtgc ggcacaacgg
     3841 caaagttata gaacggaggc ggggaagagg ttgcccactc cagcgtacgg ccaccccacg
     3901 ggtcgccagt caggtcacgg ttctggtcgc ggtcacgaat agaaacgtac atctgaataa
     3961 cgaggcagag aatacccagc gcaatcagta ccgcaccgct ggctgcaatc atcagcatgg
     4021 tgtggaactg cgggtcaatc tgctggctca aacgacgggt catgcccatg aagcccagcg
     4081 catacagtgg cataaaggca acgaagaagc cgatgatcca gaaccagaac gcgcgtttac
     4141 cccaggtttc gttcagtttg aaaccgaacg ctttaggcca ccagtaggtc atccctgcga
     4201 agcagccgaa gaccacgccg ccgatgatca cgttatggaa gtgcgcaatc aggaacaggc
     4261 tgttatgcag aacgaagtcc gcgcccggta ctgccagcag cacgccagtc ataccgccta
     4321 ccgagaaggt gacgataaaa ccgatggtcc acagcatcgc agaatggaac acgatgcggc
     4381 cctgatacat ggtgaacagc cagttgaaga tcttcacccc ggtagggatg gcgataatca
     4441 tcgtggtgat accaaagaag gcgtttacgt tcgcgcccgc acccatcgta aagaagtggt
     4501 gcagccagac gatgaacgac agcacggtga tacagacggt tgcccatacc agcgaggtat
     4561 aaccaaacag acgtttacgc gagaaggttg ccgcaatttc ggagaacaca ccgaatacag
     4621 gcaggatcaa gatgtaaact tccgggtggc cccaggccca aatcaggttg atatacatca
     4681 tcatgttgcc acccatatcg ttggtaaaga aatgggtgcc cagatagcga tccagggtca
     4741 acaacgcgac ggtaaccgtc agaattggga aggaagcaat aatcagtacg ttcgcgcaca
     4801 gtgatgccca ggtaaatact ggcatcttga acatggtcat gcccggtgcg cgcatcttca
     4861 gaatggtaac gaagaagttg ataccggtaa gcgtcgtacc tataccggat agctggagac
     4921 tccatatcca gtaatcgaca ccgactcccg gactgtactc tattcccgat agcggtggat
     4981 aggccagcca gccggtctgc gcaaattcgc ccacgccgag agaaacgtta accagaatca
     5041 caccaacaac ggtaaaccag aagcttaagt tgttgaggaa cgggaacgca acgtcacgcg
     5101 cgccgatctg cagcggaacc accaggttca tcagaccgat aacgaaaggc atcgccacga
     5161 agaagatcat aatcacgcca tgcgcggtga agatctgatc gtagtggtga ggtggcagga
     5221 agcccgcttc gcccgcagag gcaagggcct gctggctacg catcataatg gcgtcagcaa
     5281 aaccacgcag caacatcaca atcgccacga tgatatacat gataccgagg cgtttatggt
     5341 cgacggaggt cagccactct ttccacaggt aggtccactt accgaagtaa gtgatcaggc
     5401 caacgagcgc cagacctccc aaaataatgc cagcgatcgt aaccatgacg ataggttcat
     5461 ggaacgggac tgcatcaagt gataattttc cgaacatctt tattcttcct caaccccttt
     5521 aatgggcgga ttccgcgtgg ctcatgtcca tgccttccat accttccata ccttcatgtg
     5581 cgctgtgctc accttctggc tgggtcatgt ccatgctctt accgtgagcc ataaacttgt
     5641 taatcacatc ggcaaacaag tctggtttca cgttggagaa atattccacc tggttgtatt
     5701 cgctaggcgc ggccagtttt tcgaacgtag ccatgtcaga catggtgttc ggcgactgtt
     5761 tcgcttttgc gacccattgg tcgaatgcgg cgcgatccgg tgttgcaata gctttgaact
     5821 tcatgcctga gaagcccggg ccgctgtagc tggcggagat accgtcataa gtaccgggtt
     5881 cgttggcgat cagatgcagg cgagtctgca taccggccat ggcataaatc tggctaccca
     5941 gacgcggaat gaagaaggag ttcatcacgg agttggaggt cactttgaag tacaccggag
     6001 tgttcgccgg gaaagcgatt tcattcacgg ttgcaatgcc ctgttccggg tagatgaaga
     6061 accatttcca gtccatggaa accacttcga tggtaatggg cttctcgtcg tgtgccagcg
     6121 gcttgctagg ctcaagagcg tgagtggttt tccaggtcag taccgcaagg aagatgatga
     6181 ttaagatagg taccgtccag accacagctt ccactttatt ggagtgtgac cagttcgggc
     6241 tgtacttagc atctttattg ctcgcacggt acttccaggc gaaaccaaca gccatcaaga
     6301 ttgcgggaat aacgacaatc aacatcaggc caaatgccgt cagtatcagt gaacgttgct
     6361 ccagaccaat ctgtcctttg ggatctaaca gcgcagaatt acagccactg agcaatacag
     6421 tgcctgcaaa taatgacaac catcccaaac ttttattgta tttcctgagt ctcatttaac
     6481 gacctcaatt ccacgggatc tggtggcgtt taaagtgtgt gggcatttta cgggaaggtt
     6541 acattactgt aaacatgatt aaattcctgt tacttggatt tggctttcag gtcacatatg
     6601 ttgcaaaaca tatcaagttg attttaagaa gctgttgcaa aagggcgtta taacgaaagg
     6661 tgagtgcaga aggagtttgg gcgggcaatt ggtataatca atataaaata aaacaattat
     6721 ttaacaagta attatcatta gggtgacaac tgggtggtga gaaaataagg actcatcagc
     6781 tgaatcgtgt aagaaaaata gaattcataa atggcgtcaa taatttccaa acgctaagcc
     6841 gcacaaaaga acaaatatca ataagcgatg ctgatgttta tttagccgta ataattacgg
     6901 cgagtgatta ctacagctaa ataatattac agattacgtc agatgtgttt ttcgtagcgc
     6961 cagataatcc agcaaaccac caagcacgac gccagagagg gcgactaaaa ccccgacttc
     7021 cagcagcgca ggcaggaaag agaagtgcgt caaatccagc gcgtccattg tcaacagcag
     7081 taaccacacg gccaacaggc tgatgcccgc cgccagtgtc cacatggcaa aggcataacc
     7141 tgcaggatat tcggtacggg agataaagtt gtcatttact cgtgtatatt caagcgtctg
     7201 gcggcaaacc agcagcaaaa taagccctgg tacggcagcg gcgacggaga atagatagaa
     7261 tgtcgaccag ccgtgtgctt caacaaacca acccgccacg gggccgacat aaactcgccc
     7321 tacagcagaa agcgctgaga gcagggcaaa ttgagtagcg gaaaatgact tattacacag
     7381 cgtcattaac agcgcgacaa aggctgatgt acccatcccg ccacagaggt tttcgaaaaa
     7441 gacggctgcg cccatgctgt agagatgctt atcagtaatc gacagcagcc agtaaccggc
     7501 gttagatgca ccttgtaaaa tgccgaaaat cagcagtgca cggaacagcg acaggcgctg
     7561 catcaaaatc ccaccgtaca atgcgccaac aatggtcgcc agtaagccaa gcgttttgtt
     7621 aaccacgcct acttcacccg catcaaaccc gacgccgcga atcaaaaacg tggttgtcag
     7681 gctcatagcg aatgcgtcgc ccagcttata cagcacgata agaagcaaaa taagccaggc
     7741 attattgcga ccaaagaaat ctcgcagagg tgcaacaacc gcttgttcca gcgttttggg
     7801 cacaggaatg gtgtcggtgg gttctggtgc aagcaacgtc gcgataatac aggggatcaa
     7861 cagcgccgcc atcaaccagt acatgccctg ccagcccagc catttatctg ccagccacag
     7921 ggctaagccg ccggaaacca gcatccctaa acggtaaccc agcacgctga ttgccgcgcc
     7981 cgcaccacgt tcttctgccg ggagcacatc ggttttccac gcatcgaaga caatatcctg
     8041 ggaggcagag caaaaagcga tcaccaccgc cagtgccgcc atccagcgga gctgggtgcc
     8101 tggttcgaga aaacccatcg ccgcaatggc gactaataac agaatttgcg tggcgagcag
     8161 ccaaccgcgc cgccgcccga aaaatggggg cgtgtagcgg tccatcagtg gtgaccagag
     8221 gaatttaaaa acgtaagcct ggcctaccag agagaagaaa ccaatggttt tgagatcgat
     8281 attctcgacc gtcatccagg cctgtaaggt gccggacgtc agggcgagcg gtagcccgga
     8341 agcaaaaccc aggatcagca atatggctga acgcggctgt tgaaaaatac gtaaatattg
     8401 actggacatg ggcgtctaca ggcccggctt gcaccgggcc agagggcaga aattaacgcg
     8461 cgttctgctt gatgaattcg tggatgctgg tgtcctgaga catatcagcg atggtatcgg
     8521 tcagcacact gttaaccgca tcggcgatat ttttgttgga ggcctggaac gcaccttcaa
     8581 cgttgtagct ggcacgatag tttttggtca ttttgttgcc attctgcgcg gtagcgatga
     8641 tggcgatatc cgctttggtc gcgatgttgt agcgcacgtt gccctgggac acgtcagcat
     8701 acagttggct aacgatgatt tgcagattaa ccgggccatt cggaccaacc atgtatccac
     8761 gggcggtcat ttgtttttcc agcacttctt gcagcaggaa acgcagatcg cgggaggcgg
     8821 tcagggtaac gatttgatta tcgcgggtga cttttgccag cgcctgatcg gtacgctgat
     8881 cggcaccatt aatgcttacg gtgacgccca tcaggcttgg atcctgctgt ggcagtgtaa
     8941 tcgtcgggga aacttcaata gttgttggcg gttttgcgca tcctgcaagc ataaacagag
     9001 caactaacgg gaagaggatt tttttgaaca tgttcgggct ctcagagact cttaagcgtg
     9061 tttggtaaaa attcccgcca tcataacatt gccaacggcg aggggaagtg ggtaaggcat
     9121 gtaaattcat catgttgacg aaataatcgc ccctgataaa agaaacgctg atgcgaggcc
     9181 tgtgtttcaa tctttaaatc agtaaacttc atacgcttga cgggaaaacc aggacgaaac
     9241 ctaaatattt gttgttaagc tgcaatggaa acggtaaaag cggctagtat ttaaagggat
     9301 ggatgacatc tcagcgttgt cggaggagat atttcatgat gatacgtgag cggatagaag
     9361 aaaaattaag ggcggcgttc caacccgtat tcctcgaagt agtggatgaa agctatcgtc
     9421 acaatgtccc agccggctct gaaagccatt ttaaagttgt gctggtcagc gatcgtttta
     9481 cgggtgaacg ttttctgaat cgtcatcgaa tgatttacag tactttagcg gaggaactct
     9541 ctactaccgt tcatgcgctg gctctgcata cttacactat taaggagtgg gaagggttgc
     9601 aggacaccgt ctttgcctct cctccctgtc gtggagcagg aagcatcgcg taaaaacgca
     9661 tttgcaactg tcggcgcttt tccagtatgt tgctaaagat tttatgaaaa acggcctgcg
     9721 ggccgttttg ttttgtctgg attttgcgct ttttgcccag cattcagacg aaaatcgccc
     9781 gggaattgtg aaaaaatacg cgacagcgcg caataaccgt tctcgactca taaaagtgat
     9841 gccgctataa tgccgcgtcc tatttgaatg ctttcgggat gattctggta acagggaatg
     9901 tgattgatta taagaacatc ccggttccgc gaagccaaca acctgtgctt gcggggtaag
     9961 agttgaccga gcactgtgat tttttgaggt aacaagatgc aagtttcagt tgaaaccact
    10021 caaggccttg gccgccgtgt aacgattact atcgctgctg acagcatcga gaccgctgtt
    10081 aaaagcgagc tggtcaacgt tgcgaaaaaa gtacgtattg acggcttccg caaaggcaaa
    10141 gtgccaatga atatcgttgc tcagcgttat ggcgcgtctg tacgccagga cgtcctgggc
    10201 gacctgatga gccgtaactt cattgacgcc atcattaaag aaaaaatcaa tccggctggc
    10261 gcaccgacct atgttccggg cgaatacaag ctgggtgaag acttcaccta ctctgtagag
    10321 tttgaagttt atccggaagt tgaactgcag ggtctggaag cgatcgaagt tgaaaaaccg
    10381 atcgttgaag tgaccgacgc tgacgttgac ggcatgctgg atactctgcg taaacagcag
    10441 gcaacctgga aagaaaaaga cggcgctgtt gaagcagaag accgtgtgac catcgacttc
    10501 accggttctg ttgacggcga agagttcgaa ggcggtaaag cgtctgattt cgtactggcg
    10561 atgggtcagg gtcgtatgat cccgggcttt gaagacggta tcaaaggcca caaagctggc
    10621 gaagagttca ccatcgacgt gaccttcccg gaagagtacc acgcagaaaa cctgaaaggt
    10681 aaagcagcga aatttgctat caacctgaag aaagttgaag agcgtgaact gccggaactg
    10741 actgcagaat tcatcaaacg tttcggcgtt gaagatggtt ccgtagaagg tctgcgcgct
    10801 gaagtgcgta aaaacatgga gcgcgagctg aagagcgcca tccgtaaccg cgttaagtct
    10861 caggcgatcg aaggtctggt aaaagctaac gacatcgacg taccggctgc gctgatcgac
    10921 agcgaaatcg acgttctgcg tcgccaggct gcacagcgtt tcggtggcaa cgaaaaacaa
    10981 gctctggaac tgccgcgcga actgttcgaa gaacaggcta aacgccgcgt agttgttggc
    11041 ctgctgctgg gcgaagttat ccgcaccaac gagctgaaag ctgacgaaga gcgcgtgaaa
    11101 ggcctgatcg aagagatggc ttctgcgtac gaagatccga aagaagttat cgagttctac
    11161 agcaaaaaca aagaactgat ggacaacatg cgcaatgttg ctctggaaga acaggctgtt
    11221 gaagctgtac tggcgaaagc gaaagtgact gaaaaagaaa ccactttcaa cgagctgatg
    11281 aaccagcagg cgtaatttac gtagcataac gcgctaaatt cgcacaaagg cccgtcaccg
    11341 ccaggtggtg ggcttttttt tgtcatgaat tttgcatgga accgtgcgaa aagcctcttt
    11401 cggtgttagc gtaacaacaa aagattgtta tgcttgaaat atggtgatgc cgtacccata
    11461 acacagggac tagctgataa tccgtccata aggttacaat cggtacagca ggttttttca
    11521 attttatcca ggagacggaa atgtcataca gcggcgaacg agataacttt gcaccccata
    11581 tggcgctggt gccgatggtc attgaacaga cctcacgagg tgagcgctct tttgatatct
    11641 attctcgtct acttaaggaa cgcgtcattt ttctgactgg ccaggttgaa gatcacatgg
    11701 ctaacctgat tgtggcgcag atgctgttcc tggaagcaga aaacccagaa aaagatatct
    11761 atctgtacat taactctcca ggcggggtga ttactgccgg gatgtctatc tatgacacca
    11821 tgcagtttat caagcctgat gtcagcacca tctgtatggg ccaggcggcc tcaatgggcg
    11881 ctttcttgct gactgcaggg gcaaaaggta aacgtttctg cctgccgaat tcgcgcgtga
    11941 tgattcacca gccgttgggc ggctaccagg gccaggcgac cgatatcgaa attcatgccc
    12001 gtgaaattct gaaagttaaa gggcgcatga atgaacttat ggcgcttcat acgggtcaat
    12061 cattagaaca gattgaacgt gataccgagc gcgatcgctt cctttctgcc cctgaagcgg
    12121 tggaatacgg tctggtcgat tcgattctga cccatcgtaa ttgatgccag aggcgcaact
    12181 gtgccgctat acttatccag ggcggcacaa cgctgtaagc ggcttgcgcc tgagaatggc
    12241 atttgcgtcg tcgtgtgcgg cacaaagaac aaagaagagg ttttgaccca tgacagataa
    12301 acgcaaagat ggctcaggca aattgctgta ttgctctttt tgcggcaaaa gccagcatga
    12361 agtgcgcaag ctgattgccg gtccatccgt gtatatctgc gacgaatgtg ttgatttatg
    12421 taacgacatc attcgcgaag agattaaaga agttgcaccg catcgtgaac gcagtgcgct
    12481 accgacgccg catgaaattc gcaaccacct ggacgattac gttatcggtc aggaacaggc
    12541 gaaaaaagtg ctggcggtcg cggtatacaa ccactacaaa cgtctgcgca acggagatac
    12601 cagcaatggc gtcgagttgg gcaaaagtaa cattctgctg atcggtccga ccggttccgg
    12661 taaaacgctg ctggccgaaa cgctggcgcg cctgctggac gttccgttca ccatggccga
    12721 cgcaaccacg ctgaccgaag ccggttatgt gggcgaagac gttgaaaaca tcattcagaa
    12781 gctgttgcag aagtgcgatt acgacgtaca gaaagcgcag cgtgggattg tctacatcga
    12841 tgaaattgac aagatttctc gtaagtcaga caacccgtcc attacccgag acgtttccgg
    12901 tgaaggcgta cagcaggcac tgttgaaact gatcgaaggt acggtagctg ctgttccgcc
    12961 gcaaggtggg cgtaaacatc cgcaacagga attcttgcag gttgatacct ctaagatcct
    13021 gtttatctgt ggcggtgcgt ttgccggtct ggataaagtg atttcccatc gtgtagaaac
    13081 cggttccggc attggttttg gcgcgacggt aaaagcgaag tccgacaaag caagcgaagg
    13141 cgagctgctg gcgcaggttg aaccggaaga tctgatcaag tttggtctga tccctgagtt
    13201 cattggtcgt ctgccggttg tcgcaacgtt gaatgaactg agcgaagaag ctctgattca
    13261 gatcctcaaa gagccgaaaa acgccctgac caagcagtat caggcgctgt ttaatctgga
    13321 aggcgtggat ctggaattcc gtgacgaggc gctggatgct atcgctaaga aagcgatggc
    13381 gcgtaaaacc ggtgcccgtg gcctgcgttc tatcgtagaa gccgcactgc tcgataccat
    13441 gtacgatctg ccgtccatgg aagatgtcga aaaagtggtt atcgacgagt cggtaattga
    13501 tggtcaaagc aaaccgttgc tgatttatgg taagccggaa gcgcaacagg catctggtga
    13561 ataattaacc attcccatac aattagttaa ccaaaaaggg gggattttat ctccccttta
    13621 atttttcctc tattctcggc gttgaatgtg ggggaaacat ccccatatac tgacgtacat
    13681 gttaatagat ggcgtgaagc acagtcgtgt catctgatta cctggcggaa attaaactaa
    13741 gagagagctc tatgaatcct gagcgttctg aacgcattga aatccccgta ttgccgctgc
    13801 gcgatgtggt ggtttatccg cacatggtca tccccttatt tgtcgggcgg gaaaaatcca
    13861 tccgttgtct ggaagcggcg atggaccatg ataaaaaaat tatgctggtc gcgcagaaag
    13921 aagcttcaac ggatgagccg ggtgtaaacg atcttttcac tgtcgggacc gtggcctcta
    13981 tattgcagat gctgaaactg cctgacggca ccgtcaaagt gctggtcgag gggttacagc
    14041 gcgcgcgtat ttctgcgctc tctgacaatg gcgaacactt ttctgcgaag gcggagtatc
    14101 tggagtcgcc gaccattgat gagcgggaac aggaagtgct ggtgcgtact gcaatcagcc
    14161 agttcgaagg ctacatcaag ctaaacaaaa aaatcccacc agaagtgctg acgtcgctga
    14221 atagcatcga cgatccggcg cgtctggcgg ataccattgc tgcacatatg ccgctgaaac
    14281 tggctgacaa acagtccgtt ctggagatgt ccgacgttaa cgaacgtctg gaatatctga
    14341 tggcaatgat ggaatcggaa atcgatctgc tgcaggttga gaaacgcatt cgcaaccgcg
    14401 ttaaaaagca gatggagaaa tcccagcgtg agtactatct gaacgagcaa atgaaagcta
    14461 ttcagaaaga actcggtgaa atggacgacg cgccggacga aaacgaagcc ctgaagcgca
    14521 aaatcgacgc ggcgaagatg ccgaaagagg caaaagagaa agcggaagca gagctgcaga
    14581 agctgaaaat gatgtctccg atgtcggcag aagcgaccgt agtgcgtgga tatatcgact
    14641 ggatggtaca ggtgccgtgg aatgcgcgta gcaaggtcaa aaaagacctg cgtcaggcgc
    14701 aggaaatcct tgataccgac cattatggtc tggagcgcgt gaaagatcgt atccttgagt
    14761 atcttgcggt tcaaagccgt gtcaacaaaa tcaagggacc gatcctctgc ctggtagggc
    14821 cgccgggggt aggtaaaacc tccctgggtc agtccatcgc caaagccacc gggcgtaaat
    14881 atgtccgtat ggcgctgggc ggcgtgcgtg atgaagcgga aatccgtggt caccgccgta
    14941 cttacatcgg ttctatgccg ggtaaactga tccagaaaat ggcgaaagtg ggcgtgaaaa
    15001 acccgctgtt cctgctcgat gagatcgaca aaatgtcttc tgacatgcga ggcgatccgg
    15061 cttccgcact gcttgaagtg ctggatccag agcagaacgt agcgttcagc gaccactacc
    15121 tggaagtgga ttacgatctc agcgacgtga tgtttgtcgc gacgtcgaac tccatgaaca
    15181 ttccggcacc gctgctggat cgtatggaag tgattcgcct ctccggttat accgaagatg
    15241 aaaaactgaa catcgccaaa cgtcacctgc tgccgaagca gattgaacgt aatgcactga
    15301 aaaaaggtga gctgaccgtc gacgatagcg ccattatcgg cattattcgt tactacaccc
    15361 gtgaggcggg cgtgcgtggt ctggagcgtg aaatctccaa actgtgtcgc aaagcggtta
    15421 agcagttact gctcgataag tcattaaaac atatcgaaat taacggcgat aacctgcatg
    15481 actacctcgg tgttcagcgt ttcgactatg ggcgcgcgga taacgaaaac cgtgtcggcc
    15541 aggtaaccgg tctggcgtgg acggaagtgg gcggtgactt gctgaccatt gaaaccgcgt
    15601 gcgttccggg gaaaggtaaa ctgacctata ccggttcgct cggcgaagtg atgcaggagt
    15661 ctattcaggc ggcgttaacg gtggttcgtg cgcgtgcgga aaaactgggg atcaaccctg
    15721 atttttacga aaaacgtgac atccacgtcc acgtaccgga aggtgcgacg ccgaaagatg
    15781 gtccgagtgc cggtattgcc atgtgcaccg cgctggtttc ttgcctgacc ggtaacccgg
    15841 ttcgtgccga tgtggcaatg accggtgaga tcactctgcg tggtcaggta ctgccgatcg
    15901 gtggtttgaa agaaaaacta ctggcagcgc atcgcggcgg gattaaaaca gtgttaattc
    15961 cgttcgaaaa taaacgcgat ctggaagaga ttcctgacaa cgtaattgcc gatctggaca
    16021 ttcatcctgt gaagcgcatt gaggaagttc tgactctggc gctacaaaat gaaccgtctg
    16081 gcatgcaggt tttgactgca aaatagtgac ctcgcgcaaa atgcactaat aaaaacaggg
    16141 ctggcaggct aattcgggct tgccagcctt tttttgtctc gctaagttag atggcggatc
    16201 gggcttgccc ttattaaggg gtgttgtaag gggatggctg gcctgatata actgctgcgc
    16261 gttcgtacct tgaaggattc aagtgcgata taaattataa agaggaagag aagagtgaat
    16321 aaatctcaat tgatcgacaa gattgctgca ggggctgata tctctaaagc tgcggctggc
    16381 cgtgcgttag atgctattat tgcttccgta actgaatctc tgaaagaagg ggatgatgta
    16441 gcactggtag gttttggtac ttttgccgtc aaagagcgtg ctgcccgtac tggccgcaac
    16501 ccgcagaccg gtaaagagat caccatcgct gctgctaaag taccgagctt ccgtgcaggt
    16561 aaagcactga aagacgcggt aaactaagcg ttgtccccag tggggatgtg acgaagttca
    16621 agggcgcatc tactgatgtg ccttttttat ttgtattcgg tgactttctg cgtcttgtgg
    16681 gctgacaatt gcccccgttt cttgtcacaa taggcctttg cgcgcatcga tacgttgcgt
    16741 gaggtacaca gtcatctaca gcggagtgtt gttacaccat gatggacagc ttacgcacgg
    16801 ctgcaaacag tctcgtgctc aagattattt tcggtatcat tatcgtgtcg ttcatattga
    16861 ccggcgtgag tggttacctg attggcggag gcaataacta cgccgcaaaa gtgaatgacc
    16921 aggaaatcag ccgtgggcaa ttcgagaacg ccttcaacag cgagcgtaat cgcatgcagc
    16981 aacagctggg cgatcaatac tccgagctgg cagcgaacga aggctatatg aaaaccctgc
    17041 gtcaacaggt gctgaatcgt ctgatcgacg aggcgcttct ggatcagtac gctcgtgagc
    17101 tgaaactggg tatcagcgat gagcaggtta aacaggcgat tttcgcgacc ccagccttcc
    17161 aggttgacgg caaatttgat aacagccgct ataacggtat cctcaaccag atggggatga
    17221 ccgccgatca gtacgcccag gcgctgcgta accagctcac tactcaacag ctgattaacg
    17281 gcgttgccgg taccgatttt atgctgaaag gtgaaaccga cgagctggcg gcactggtag
    17341 ctcaacaacg cgtggtgcgt gaagcgacta tcgatgttaa cgtgctggcg gcgaagcagc
    17401 ctgtgaccga acaggaaatt gccagctact acgaacaaaa caaaaacaat ttcatgacgc
    17461 cggaacaatt ccgcgtgagt tacatcaagc tggatgccgc gacgatgcag caaccggtta
    17521 gcgatgcgga tatccagagc tactacgatc agcatcagga tcaattcacc cagccgcagc
    17581 gtacccgtta cagcatcatc cagaccaaaa ctgaagatga agcgaaagcg gtacttgatg
    17641 agctgaataa gggcggtgat tttgctgcat tagccaaaga aaaatctgcc gatattatct
    17701 ctgcccgcaa cggcggtgat atgggttggt tagaagatgc cactatcccg gacgaactga
    17761 aaaatgcggg tctgaaagaa aaaggccaac tgtctggtgt tatcaaatct tcggtcggtt
    17821 tcctgattgt gcgtctggac gacattcagc cagcgaaagt gaaatcgtta gacgaagtac
    17881 gtgacgacat tgcggcgaaa gtgaaacacg aaaaagccct cgatgcgtac tacgcgctgc
    17941 agcagaaagt gagcgatgcg gcaagcaacg acaccgagtc tctggccggt gcagagcaag
    18001 ctgccggcgt taaagccact cagacgggtt ggttcagcaa agataatctg ccggaagagt
    18061 tgaacttcaa gccagttgcc gacgctatct tcaacggcgg tctggtaggt gaaaacggcg
    18121 cgccgggcat caactctgac atcatcaccg tagacggcga ccgcgcattc gtgctgcgca
    18181 tcagcgagca caaaccggaa gcggtgaaac cgttggcaga tgttcaggaa caagttaagg
    18241 cactggttca gcacaacaaa gctgaacaac aggcgaaagt ggatgctgag aaactgctgg
    18301 ttgatttgaa agccggcaaa ggtgcggaag ctatgcaggc tgccggtctg aaatttggcg
    18361 agccgaaaac cttaagccgt tccggtcgtg acccgattag ccaggctgcg tttgcactgc
    18421 cactgccagc gaaagacaaa ccgagctacg gtatggcgac cgatatgcaa ggtaatgtag
    18481 ttctgctggc gctggatgaa gtgaagcaag gttcaatgcc ggaagatcag aaaaaagcga
    18541 tggtgcaagg tatcacccag aacaacgcac aaatcgtctt tgaagctctg atgagtaacc
    18601 tgcgtaaaga ggcgaaaatc aaaattggcg atgcgctgga acagcaataa tcctgaagcc
    18661 gcctcgcaaa aaaatgcttt gcagctgtaa caagaaaagg tcgctttcgc ggccttttcc
    18721 atttctgaac attgccattt gtttactgtt ttcactgccg ttaaggtgat tccactgtta
    18781 acaaacaagg agaaaacagt atgaaacacg gaattaaagc actgctcatt accctgtccc
    18841 tggcctgtgc cggaatgtct catagcgcgc tggcggcagc ttctgtggcg aaaccgacaa
    18901 cagtagaaac caaagcggaa gctcctgcag cacaaagtaa agcagtaccg gcgaaagcca
    18961 gtgacgaaga aggcacccgg gtcagcatta ataatgccag cgcggaagag ttagcccgcg
    19021 cgatgaatgg cgttggcctg aagaaagcgc aggcgattgt cagttatcgc gaagagtacg
    19081 gtccgtttaa aacggtggag gatctaaaac aggtgccggg gatgggcaat tcgctggtgg
    19141 aacgtaatct ggcggtatta accctgtaat taatttgcat agtggcaatt ttttgccaga
    19201 ctgaagaggt cataccagtt atgacctctg tacttataac agttcttata acaacaacgt
    19261 aaggttattg cgctatgcaa acacaaatca aagttcgtgg atatcatctc gacgtttacc
    19321 agcacgtaaa caacgcccgc tatctggagt ttcttgaaga agcccgctgg gatggactgg
    19381 aaaatagtga cagctttcaa tggatgactg ctcacaacat cgctttcgtg gtggtcaata
    19441 tcaatattaa ctatcgtcgc ccggcggtat taagcgactt gttaaccatt accagtcagt
    19501 tacagcaatt aaacggtaaa agcggcatct taagccaggt cattacactg gagccggaag
    19561 ggcaggtagt agcggatgcg cttattacgt ttgtttgtat tgatcttaaa acgcagaaag
    19621 cattagctct ggaaggagaa ttgcgcgaaa agctggagca gatggttaag taaacgtttt
    19681 gtggtgccgg atgctcaagc cgcatccggc gacacccgga ataattaatt caacccggtt
    19741 ttctgcttca tcgctgccat caccgtcggt ttatcggcca gataatgatt caaaccgttg
    19801 gcacgtaaat tacacgccgc acaatgaccg caaccgtcgc ctttaatgcc gttataacag
    19861 gtcagcgttt cgttacggac taaatccagc ttgccgtaat aatctgccag cgcccaggtt
    19921 tccgctttat caatccacat cagcggcgtt tcaaaacgaa tatctttcgc catgcccaaa
    19981 ctgacggcat ggtttagtgc tttcacaaac tcatcgcggc aatccgggta gccggagaaa
    20041 tccgtttcgc agacgccggt aattacggct tctgctttta cctgatacgc atatattgcc
    20101 gccagcgtca ggaacaaaat attacgccct gggacaaacg tattcgggat accatcggct
    20161 tcaggttcat aatcaggcac cggaatgctg tcacgcgtca ggctgctgac cgccagctcg
    20221 ttgagcagcg ttacatcaag caccttatgc gcgcgtgccc ccagtttcag cgccagttcg
    20281 cgtgccacgt cgatttctgc gcgatggcgt tgaccgtaat cgaacgtcac gcaatggact
    20341 tcatcatatt gttgtaatgc ctgcaccaga caggtggtgg aatcctggcc tccactgaac
    20401 acaacgacag cacgtttcat agataatcct gcttgaacaa taaaagcgtt atggtaacgc
    20461 ctgcgattaa cccggaccag ctattcattg cgacggcgcg ggaagccagg cttcggtaaa
    20521 ttcaaaccag ccgcgcggtg tcagtcgcac accgttcacg ccgggagggg cgctaatgcg
    20581 atagtgatag ttgaacagcg gtgtcagcgt cgcatctgcc attaactggc taaaaaccgc
    20641 tttcagggca ttaaatcggt tttcctcatc aggcattact tgcaccgcgt ccagtgtcga
    20701 ttgcaaatgt gcatatgctg gagcgtcgaa aacatgtggc cacagtggat cgcaacgcag
    20761 ccattgctcc agggtatatt ccggtgcttc gccaattaat ctgtcgccca tcatgaggtc
    20821 tgcgtgtgcc agtagggtcg tgtcgtccca gttttttgcg ttatgaaaaa taattgtaag
    20881 ttcacagcct tccgctgcca gtgtcgcctg taggcgttct gccatggtat gaagttctat
    20941 cggtaggtga taaaccagcg tcaaggtttt cggtagtttg acttcatccg gtacttgcca
    21001 ttgtggaata gtccagtctg gcagtaatgc atgactggcg gtgatcaggt tttctccgac
    21061 ttctaacgtt tgtaataaac cggattgatg aataatggag atcacttttc gcgcctgcca
    21121 gagggacagt cggggacttt tgcgcaacgt taaatagcaa aaacctaaac tgatgccact
    21181 actgacctgg ctgacccgtt gcagctcctc aggtttgccg atggtgattt gcacgggatg
    21241 ccgacaactg gttcccaaat ctttttcgaa aagtggcgga gttatccagt actcaaccgc
    21301 tttaagcagc ggatgacgta aatggtaata atcatggctt tccaggcgca ccagctccgc
    21361 tgtgaattgt gttaagcgaa aaggaccagt gccgatcagt gggaattgcg gatgcgccag
    21421 atggctgcaa tagctcgcca gtcggtgcgc aagccagtaa tcggggcgat gtaaaaagaa
    21481 ggtcagacac tgcggatggg tgacttcaat acgcttcacg ctaataaata attgatccag
    21541 tgctggcagt tgtaacagca tcaataatcg ctggtgtaag tgtgaggctt ttactgcatc
    21601 gccgttatgc cagtgtagcg ttgaacgaag ataaaagtcc cagcgtaacc cgtcagtaga
    21661 ggtttcccag tgatgtgcta aatcgccaat cgggcgctga gtattattat cgaagcgggt
    21721 caggccggaa aatatctgcc cggccagatg ctgctcggca cggccgggca aaaagcccgg
    21781 ttgtagcggt tcgagcgggc gatagtaggg aatacgcaac gtgggcgtat cgttttgcca
    21841 ttgtccgccc ataaacggct gtaacagagt gcgcaactca cctggggcca gttgcgccag
    21901 ctccagcaca tcttgctgct ttccggtttc cagcgcctgt tccatcatcg cattgcgtag
    21961 cgattctggc gtgaccagaa agcgtagttg accgcgcttt ccgcgtcctg actgcgcctg
    22021 ccactccagc catcccgcct cctgtgcctg gcgtaacagc gtacgaacat ggcgttcgct
    22081 gcaaaaacag cgttcggcca gttcgctgac ggtgacggtt tgcggctttc cggcggaagg
    22141 ttgccacaga cgttgatact ggttaagacg gttgagcagt cgcatataaa cccggaacaa
    22201 tattatttaa ctattcacta ttacttccgt atatatcaga tgatactcaa tcaccattaa
    22261 ccgtgtcaca gagtggagaa agaaatggct cgtctggcag catttgatat ggatggcact
    22321 ttattgatgc ctgaccatca tttaggtgag aaaaccctct ctactttggc gcggctgcgt
    22381 gaacgcgaca ttaccctcac ttttgccacg gggcgtcatg cgctggagat gcagcatatt
    22441 ctcggggcgc tatcgctgga tgcatatttg attactggca acggaacgcg cgtgcattct
    22501 ctggaaggtg aacttttaca tcgtgatgat ttacctgcgg atgtcgcgga gctggtgctg
    22561 tatcagcaat gggatacccg agcaagcatg catatcttca atgacgatgg ttggtttacc
    22621 gggaaagaga tccctgcgtt gttgcaggca tttgtctata gcggttttcg ttatcagata
    22681 atcgatgtca aaaaaatgcc actcggcagc gtcaccaaga tctgcttctg tggcgatcac
    22741 gacgatctta cacgcttgca gatccagcta tacgaagcat taggcgagcg tgcacattta
    22801 tgtttttccg ccacggattg cctcgaagtg ctgccggtgg gctgcaataa aggcgctgca
    22861 ttgacggtgc tgacccaaca tttaggttta tcgttacgcg attgcatggc ctttggtgat
    22921 gcgatgaacg atcgcgaaat gttaggcagc gtcggtagcg gatttattat gggcaatgcg
    22981 atgccgcaac tgcgcgcgga gctcccgcat ttaccggtga ttggacattg ccgaaatcag
    23041 gctgtctctc actatttgac gcactggctg gactatccac atctacctta ttcccccgaa
    23101 taacgagatc ccttccagca ccgggcaatt gcccggtttt ttttgcgttg aatttgtcat
    23161 tttgtgccgt gatgcttaaa ccgcacagaa taaattgtcg cgattttacc tttaaaatag
    23221 aattaaaaga gaaaaaaatt ctctgtggaa gggctatgtt agataaaatt gaccgtaagc
    23281 tgctggcctt gctacagcag gattgcaccc tctctttgca ggcactggct gaagccgtta
    23341 atctgacaac caccccctgc tggaagcgtc tgaaacggct ggaggacgac ggtatcctta
    23401 tcggcaaagt cgccctgctg gatccggaaa aaataggcct cggcctgacc gcctttgtgc
    23461 tgataaaaac gcaacatcac agcagcgaat ggtattgccg ctttgtcacg gtggttaccg
    23521 aaatgccaga agtgctgggg ttctggcgca tggctggtga atacgattat ctgatgcgcg
    23581 tccaggttgc cgacatgaaa cgctacgatg agttttataa gcgtctggta aacagtgtac
    23641 cggggctgtc ggacgtcact tccagcttcg cgatggaaca gattaaatac accacttctt
    23701 tacccatcga ataaatatcc agaatcaagt caggacacaa cgcgtgcgat tatttgctca
    23761 attaagctgg tatttccgtc gggaatggcg tcgctatctc ggggctgtcg ccttgcttgt
    23821 cattatcgcg atgctgcaac tggttccgcc aaaagtggtt ggtattgttg ttgatggcgt
    23881 gacagaacaa cactttacta ccgggcagat cctgatgtgg atcgccacca tggtgctgat
    23941 tgccgttgtg gtttatctcc tgcgttacgt ctggcgcgta ttgctgtttg gtgcgtctta
    24001 tcaactggct gttgaactgc gtgaagatta ttaccgccag ctaagccggc agcatcctga
    24061 gttttacctg cgtcatcgca cgggtgacct catggctcgt gcgaccaatg acgtcgatcg
    24121 cgtcgtgttt gccgccggag aaggggtgct aacgctggtg gattcactgg taatgggctg
    24181 cgctgtgttg attatgatgt ctacgcaaat tagctggcag ttgaccttat ttgccctgtt
    24241 gccgatgcca gtgatggcga tcatgatcaa gcgcaacggc gatgccttgc atgaacgctt
    24301 taagctggca caggcggcgt tttccagtct taatgaccgc acccaggaaa gcctcaccag
    24361 tatccgcatg atcaaagcct ttggtctgga agatcgtcag tcggcgttat ttgccgcgga
    24421 tgccgaagat accggcaaaa aaaacatgcg ggtggcgcgt attgatgctc gtttcgaccc
    24481 gaccatctat atcgcgattg gtatggcgaa cttgctggcg attggcggcg gtagttggat
    24541 ggtggtacag ggcagtttaa cgctgggcca gctcaccagt tttatgatgt atttaggtct
    24601 gatgatttgg ccaatgctgg cgctggcatg gatgtttaac attgtggaac gtggcagcgc
    24661 ggcgtatagc cgaattcgta ccatgctggc cgaagcgcca gtcgttattg atggtagtga
    24721 taaggtgcca gaaggtcgtg gtgaactgga tgtgaatatt cgccagttta cgtatccgca
    24781 gactgaccat cctgcgctgg aaaacgtcaa tttcgccctg aaacccggtc agatggtagg
    24841 tatttgcggg ccgactggtt ccggcaaaag taccctgttg tcgctcattc agcgtcattt
    24901 cgacgtcagc gagggggata tccgctttca tgatattcct ctgacgaagt tacaactcga
    24961 tagctggcgt agccgcctgg cggtagttag ccagacaccg ttcctttttt ccgacaccgt
    25021 agcgaacaac atcgcgttgg gttgcccgaa tgccacccag caagagattg agcatgtcgc
    25081 gcggttagcc agcgtgcatg acgatatttt gcgtttaccg caaggttacg atacagaagt
    25141 gggcgagcgc ggcgtgatgc tttccggcgg gcaaaaacag cgtatctcta ttgctcgtgc
    25201 gttattagtc aacgcggaaa tcctcatcct tgatgatgcg ctttcggcgg tggacggacg
    25261 cacagagcac cagatcctgc ataacctgcg tcagtggggg cagggaagaa cggtaatcat
    25321 cagtgcccat cgcctttcgg cactgacgga agccagtgaa attattgtga tgcagcacgg
    25381 acatatcgct cagcgtggca atcatgatga gctggcgcaa caaagtggct ggtatcgcga
    25441 tatgtatcgc tatcaacaac tggaggcggc gctcgacgac gctccggaaa ttcgcgagga
    25501 ggccatcgat gcgtagtttt agccaactgt ggccgactct caagcgcctg ttagcgtacg
    25561 gttcaccgtg gcgtaaaccg ttggggattg cggtcctgat gatgtgggtt gcggcggcgg
    25621 cagaagtcag tgggccgctg cttatcagct attttatcga caatatggta gcgaaaaata
    25681 acctgccgtt gaaagtggtt gcagggctgg cggcggcgta tgttgggctg caactgtttg
    25741 cagccgggct acattacgcg cagtcgctgc tgtttaatcg ggcggcagta ggcgtagtgc
    25801 aacagttgcg taccgacgtg atggatgctg cgttacgcca gccattaagc gagtttgata
    25861 cccaacccgt cgggcaggtg atttcccgcg tcactaatga cactgaagtg atccgcgatc
    25921 tctacgttac cgtagtggca actgtcctgc gtagcgccgc actggtgggc gcgatgctgg
    25981 tggcgatgtt cagcctcgac tggcgaatgg cgctggtggc gataatgatt ttcccggtgg
    26041 tgctggtggt aatggtgata tatcagcgtt acagcacgcc gattgtccgt cgtgtgcgcg
    26101 cttatctggc ggatatcaac gacggcttta acgaaatcat caatggcatg agcgttatcc
    26161 agcagttccg tcagcaggcg cgatttggcg aacgtatggg tgaggccagt cgttcacact
    26221 atatggcgag gatgcaaacc ctgcgcctcg acggttttct gctgcgtccg ctgctgagtc
    26281 tgttttcatc gctcattctt tgtggcttgt tgatgctgtt tggcttctcc gccagcggca
    26341 ccattgaagt gggcgtgttg tatgcgttca tcagttattt ggggcgactt aacgaaccct
    26401 taatcgaact gaccacgcag caggcgatgc tgcaacaggc tgttgttgct ggtgagcgcg
    26461 tttttgagct gatggacgga ccgcgccagc aatatggcaa tgatgatcgc ccgttgcaga
    26521 gcggcaccat cgaagtcgat aacgtgtcat ttgcttatcg cgatgacaat atggtgctaa
    26581 agaacattaa tctctctgtg ccttcgcgca attttgtggc gctggtcggg cataccggca
    26641 gtggcaaaag tactctcgcc agtttattga tgggctatta cccgctaacg gaaggtgaga
    26701 ttcgccttga tggtcgtcca ttaagttcgc taagtcacag cgcgctgcgc cagggcgtgg
    26761 caatggtgca gcaagatccg gtggtgctgg cggatacctt cctcgccaac gtgacgctgg
    26821 ggcgtgatat ctccgaagaa cgcgtctggc aggcgctgga aaccgtgcaa ctggcggagc
    26881 tggcgcgtag catgagcgac ggtatttaca cgccgcttgg cgagcagggg aataatctct
    26941 cagtcgggca aaagcaactg ctggcactgg cacgcgtgtt ggtggagacg ccgcaaatcc
    27001 tgatccttga tgaggcaaca gccagcattg actccggtac tgaacaggcg attcaacatg
    27061 ctctggcggc ggtgcgtgag catactacgc tggtggtgat tgctcaccgc ttatcaacta
    27121 ttgttgatgc cgacactatt ctggtgcttc atcgtgggca agccgtggag cagggcactc
    27181 accagcaact gctggcggcc cagggacgct actggcagat gtatcaactg caacttgcgg
    27241 gcgaagagct ggcagccagc gtgcgtgaag aggaatcatt gagcgcctga atagcgcaat
    27301 atttcatcgt tggtgcaaaa atgtaacgca ctgtgcactg tcatagtgcg ttttcatttt
    27361 caatcttctt aacttcctgc tctctttctc gtttttcatt tctggcacac cgcttgcaat
    27421 accttcttcg tgtagcagaa ccattaccga attctgaccg gaggggatct atgaagctgg
    27481 tgaccgtgat aatcaaacca ttcaagctgg aagacgttcg tgaagcgtta tcttccattg
    27541 gtattcaggg cctgaccgtc accgaagtga aaggtttcgg gcgtcagaaa ggacatgccg
    27601 agctgtaccg gggggcggaa tacagcgtca atttcctgcc aaaagtaaaa attgatgtgg
    27661 cgattgctga tgaccaactc gatgaagtga tcgatatcgt cagtaaggcg gcttacaccg
    27721 gaaaaattgg cgacggcaaa atcttcgtcg ctgaattgca acgcgtcatt cgtattcgta
    27781 ccggcgaagc cgacgaagcg gcgctgtaat ctctggcaca cagcaacagg aacgaaaaat
    27841 gaagatagcg acgataaaaa ctgggcttgc ttcactggcg atgctgccgg gactggtaat
    27901 ggctgcacct gcggtggccg ataaagccga caatgcgttt atgatgattt gtactgcgct
    27961 ggtgctgttt atgactattc cggggattgc cctgttttac ggtgggttga ttcgcggcaa
    28021 aaacgtgctg tcgatgctga cgcaggtgac ggtgacgttt gcactggtct gtattctctg
    28081 ggtggtttac ggttactcgc tggcgtttgg tgagggcaac aacttctttg gcaacattaa
    28141 ctggttgatg ctgaaaaaca tcgaactgac ggcggtgatg ggcagcattt atcagtatat
    28201 ccacgtagcg tttcagggat cgtttgcctg cattaccgta ggcttgatag ttggagcact
    28261 ggcggaacga atccgcttct cagctgtgtt gattttcgtg gtggtatggc tgacgctctc
    28321 ctacattccg attgcgcata tggtctgggg cggcggtttg ctggcttctc acggtgcact
    28381 ggattttgcg ggtggcactg tggtgcacat taacgccgca atcgccggtc tggtgggcgc
    28441 gtatctgata ggcaaacgcg tgggcttcgg caaagaggcg tttaaaccgc acaacctgcc
    28501 gatggtcttc accgggactg ccattctcta tatcggttgg tttggcttta acgccgggtc
    28561 agcgggcacg gcgaatgaaa tcgcagcact ggcatttgtg aatactgtgg tcgcaacggc
    28621 ggcagcaatt cttggctgga tcttcggtga atgggcgctg cgcggtaagc cttcactgct
    28681 gggagcgtgt tctggcgcga ttgccggtct ggtcggcgtg acgccagcgt gtggctacat
    28741 tggtgttggc ggcgcgttga ttatcggcgt ggtagctggt ctggcgggct tgtggggcgt
    28801 taccatgctc aaacgcttgc tgcgggtgga tgatccctgc gatgtcttcg gtgtgcacgg
    28861 tgtttgtggc attgtcggct gtatcatgac cgggattttt gccgccagtt cgctgggcgg
    28921 cgtgggcttc gctgaaggtg tgacgatggg ccatcagttg ctggtacagc tggaaagcat
    28981 cgccattacg atcgtctggt ccggtgtggt ggcgtttatt ggctacaaac tggcggatct
    29041 gacggttggt ctgcgtgtac cggaagaaca ggagcgagaa gggctggacg tcaacagcca
    29101 cggcgagaat gcctataacg cgtaacaagc actgcaaaaa acagccggac ggttttcacc
    29161 tccggctatt tttttaattg tgattacgca ttaccccttc ctgaacggtc gaggcaacca
    29221 gcacgccgtc ctgagtataa aactcaccgc gcacaaagcc gcgtgcgctg gacgccgagg
    29281 tgctttccac gctatacagc agccattcat tcaaattaaa cgggcgatgg aaccacatgg
    29341 aatggtcaat ggtggcaatc tgaatccctg gttcgagaaa accgatgccg tgcggctgta
    29401 gagctaccgg caggaagtta agatcagaag cgtaaccgag cagatactga tgaacgcgca
    29461 gatcatccgg cacgctacca tttgcgcgga tccacacctg acgatgcggt tctgcgacgt
    29521 gacctttcag tgggttatga aactccaccg gacggacttc cagcggacga tcgcagatga
    29581 atttatcttt cagcactggc ggcagcaggt gcgccagcga ttgggcgatt tgcgtttctg
    29641 aaggcaggcc atcaggcgct ggcgcggacg gcattgtttt ttgatgttcg aaacccgctt
    29701 ctggtgcctg gaaagaggca gtcatataaa aaatcggttt gccgttttga atagcagaaa
    29761 cccggcgggc gctgaagctg ttaccgtcac gcagcgtttc gacatcataa ataatcggct
    29821 tcttactatc gccagggcga agaaagtagc tgtgaaaaga atgtaccagt cgctcgtcag
    29881 ggacggtctc ttttgcagca tacaaggcct gacccacgac ctggccgcca aatacctggc
    29941 gtaaacctaa atcttcactc tggccgcgaa agagtccttc ctcaattttt tccagattta
    30001 acaatgtcag taaatttttt agcgcctgac tcatataact ctccagtaac aaagctgccg
    30061 cagcaagcca aagtgagttg agtataacgc aaatttgcca ctggtccgat gggtgcaatg
    30121 gtctgaatta cgggctaatt acaggcagaa atgcgtgatg tgtgccacac ttgttgacgt
    30181 tactattttg ttaaccactc ttccggcgag gaaagttagc ccgctggtgc attgataata
    30241 aggagaagtg aatgaaactc gtgcacatgg ccagtggttt agcggttgcg attgcgttgg
    30301 cggcttgcgc agataaaagc gcggatattc agacgccagc accggctgca aatacgtcta
    30361 tttcagcaac acaacaacct gctatccagc aaccgaatgt ctccggtacc gtttggatcc
    30421 gtcagaaagt cgcactgccg cctgatgccg tgctgaccgt gacactttct gacgcgtcgt
    30481 tagccgatgc gccgtcgaaa gtgctggcgc agaaagcggt gcgtaccgaa ggtaaacagt
    30541 caccattcag ctttgttctg ccatttaacc cggcagatgt tcagccgaac gcgcgtattc
    30601 tgttgagtgc ggcgattacc gtgaatgaca aactggtatt tattacagat accgttcagc
    30661 cggtgatcaa ccagggcgga actaaagccg acctgacatt ggtgccggtg cagcaaaccg
    30721 ccgtgccggt tcaggctagc ggtggcgcaa cgactaccgt accttcaact tcaccaaccc
    30781 aggtgaatcc gtcttcggca gttccagctc caacgcaata ttaagcgaag ttaaccctct
    30841 ccgcccggag agggttagta gttccagcga taaagctgca agtcgatttg cccgcttccc
    30901 gacaccatca caccttctgc cagtaatgcc tgtcgctgac gctgtaaatc cggtccggtc
    30961 agtgaaatgg tgccgtggcg attaaccacc cggtgccagg gcaaggtgct gccttcgggg
    31021 agacgcttta acacaccgcc tacctggcgc gcggcacggg gcgatcccgc cagtttcgcc
    31081 acatcaccgt aagtggtgac atagccttcg ggaatagcgg ctacgatttg ccagacgcgt
    31141 tggggaaatg aatcttcttt ttccatcttt tcttcctgag gtaatttttc agcataatct
    31201 ggaaaaacgc ccgagtgaag ccgcattgcg caagaaacca gcatctggca cgcaatgggt
    31261 tgcaattagc cggggcagca gtgataatgc gcctgcgcgt tggttctcaa cgctctcaat
    31321 gggggctctg ttggttctcc cgcaacgcta ctctgtttac caggtcaggt ccggaaggaa
    31381 gcagccaagg cagatgacgc gtgtgccggg atgtagctgg cagggccccc acccatttct
    31441 gccgcccacc gtttcgtcaa aaaatcccaa catggctaaa ctttaaccac aactgacgtc
    31501 gcaagaattg tctggctgcg cagtacgctt cggaggtatg tctgatgaag tatgttgatg
    31561 gttttgttgt tgccgttcct gccgataaaa aggatgccta tcgggaaatg gccgctaagg
    31621 ccgcgccatt atttaaagag ttcggtgcgc ttcgtattgt cgaatgctgg gccagcgatg
    31681 taccggatgg taaagtgacc gattttcgta tggcggtgaa agcggaagag aatgaagagg
    31741 ttgtctttag ctggattgaa tacccttcaa aagaggtccg cgacgctgct aatcaaaaga
    31801 tgatgtcgga cccacggatg aaagagttcg gcgagtccat gccgtttgac ggcaaacgaa
    31861 tgatctatgg tggattcgaa tcaatcatcg acgagtaggt agcgtgacgg gctgtgcggc
    31921 gtgccgtcag ccctaaaatt cacaaatgtt gctcggccca gcgtaagaaa tcctctttcg
    31981 gtaatgcctt gctgtagagc cagccctgac cgtagtgcac gccatgctgg cgtagccact
    32041 cttcttgttt actggtttcg attccctccg caaccatttt cagtttcagt gtttttgcca
    32101 tttcgatgat atgcggcgtg acatttttat attccagcgc atcaacgaaa gatttatcga
    32161 tcttcagaat gtcgacatcc agatcctgta aatagcttaa acttgaatac cctgtaccaa
    32221 aatcatcgag ataaatttca tggcccgcct cccggtagcg agaaattatc ggggcgctgg
    32281 ttttcggatc ggcaaactcg cgttcagtga gttcaagcgc gatctgtctg ggattaacct
    32341 gatagtgatt gatcatttca cgcagcaatt gcgggatttt ttccgaggtg agcacggtgg
    32401 attcaagatt gatcgaaata tgctgctgag gatgctgacg cagccagtcg cccatatctt
    32461 caaagacgct tcttataatc agtagcgtca atggctcaga aaggcccgtt tgctgtgcca
    32521 gcggaataaa actatctggt gacaaccaac taccgtctgt ctgcggccag cgcgccagtg
    32581 cctcagcacc gacaattttg ccattggcta aggagacaat cggctgatag tgcacgcaaa
    32641 tatcacgatt ttcgatagca tcctgcagcc gatgatgcgg tgactggata cggcgcaaaa
    32701 tacgcagcac aaacatcgct gccagcaggc caatcaccaa cccgagcggt aaccaaataa
    32761 agacttgccg atgccagcct ttctgcaaca ttttcgttga agcccaggtg atgatcgaaa
    32821 tattcatctc cggtaagggc aggatatcgt aaatgattcc attattttcg atatgctcac
    32881 cgggtgtttt ttgtagcctg gtaataattc cctgagcaag ttcatcgctg ctggttatga
    32941 caacgttatg ggcattgcca ataatggcgg catcaatttg ccatgagcta tagggaatga
    33001 catcaataaa ggaagcgggg tcgatcatga cgacataatg tgccgttccc atggcgacca
    33061 tgtaacgaat aatgcctaaa tcgttatgcg atgttaacca gacacgataa ccatctttcg
    33121 aaattttacc tggctcgggg aaggtatcgg gcgggctttc atgctccaga gacgaacact
    33181 ggggaacgtt gttatcgata taaaccactt cctgaatata gcgataactg taagataccc
    33241 ggcgcatttc catgagatgg gcttcgctac aggcagcgcc ttgccatctt tccagctctt
    33301 gcagcgcatc tttcccttgt gtcgccacct tattggcccg aatagcgacg cgggaggaat
    33361 acgtattcag ctcttcaata aatgatgttt ctacctgctg atgggccagc cagatgctta
    33421 agccgacagg cagcaatact gaaagaatca gtactcccga aataaggccg accagatgtc
    33481 gtgttctcac cagcatatcc ttattaacac agtgtaggga aagtatatct gattgtggaa
    33541 aaaggctggc aggagataag gaggtagagc tggtatttca tgaagtgatg ggtggaagcg
    33601 atcgttgtca tttgaccagg cgtagagctt tacgcctggt atgtacccct aacttaagat
    33661 gttgattcgg cgcgagcgag ggtaaaaata tcgtaaaaag atagttcacc tttacggacg
    33721 atcatttttt gcagttgttc tttatgaaca tccgcgacac gcggagagtt caggatggct
    33781 tcaactagcg cggcaggtac aaagcgcgtg ttgtaccatt cgccgttata gcaaacgcgg
    33841 aaatccagca cgtcgatatc ttcatagcgg tagcgtgggt agacatcgaa aaagaagaaa
    33901 ccattaagca ggataaataa tacaaccagt agccagacag agcctgaaag cgtttcggac
    33961 tgcaacatca ccgccagggt ggcaaaaaaa gcaacgtaca taccgataaa cagccccgga
    34021 tgtttacgga taaaactgat actaaagcgg ggtttgttgt cgcgtttttc gcgggtattc
    34081 agagactcaa tcgtttcggt cagcaggcgt tgtatttcgg tcatcgtttg ccttcgtgat
    34141 atctgcaact ttccaggtaa caccctattt taacgtggga atagtagagg ggaagtaaca
    34201 gatttgccac gattttgcat aacagttgag aaaagctggt tacaattttt taatgattct
    34261 ggctggatta ccgcccacga caacgttgtc cgggacatct tttgtgacaa ctgcacctga
    34321 ggctaccacg acgttatcac caatggtcac accagggtta atgactgcac gtccgccaat
    34381 ccagacgtta ttaccgatgg tgacgggttt ccccagttca gcaccgctat tacgtgctac
    34441 agggtcgatg ggatgtgttg ccgtgtagat atgaacgcct ggtgccaaca tacagttatc
    34501 accaatgcga atagggcaga catcaagcat cacgcaatcg aagttggcga aaaaattatt
    34561 gccgagaaaa atgttataac catagtcaca gcgaaacgtt ggctcaatat aagcctctgt
    34621 cacctgaccg aataaatcag caagaatttg ctggcgtaat gtgtgctctt ccgccaggga
    34681 atgattgtat cggtgaataa gccgacgagc tcgcaggcga tcgcgagata acgcctcatc
    34741 tgccgagtga tacaactcac cagcaatcat cttttctttt tctgtgctca ttactgactt
    34801 ccggttgctg aaaatgcggc aacacagtaa gacgaaaagt cagtgaagag aacgttccca
    34861 ttacatctgt gtgatgaaga tcacataggg tatgccttca tggcgaaaaa attataaaac
    34921 tcttaataaa cagccggtta tagctccgaa agcgagttat ttattagcga ataaatttcc
    34981 atactgagga agggatcttg tcgtacagtt tattcatggt caattcggcg aggcggtgat
    35041 ctgcggctga gtaaaatacc gccagttcat tatctgataa ttcgtattta tttttctcga
    35101 taacacgctc cagcgtgtca attgtctgac aacgacgtaa acgcattaaa taatcggttt
    35161 tcgttaaagg tttttcggac atacttctac ccatggttgt aataattcta acaagataaa
    35221 ctcgcaggat tctctttcgt cgcattgaca aaacaacgga atagtcgatt acctgacttc
    35281 cgccatttct gaagatcctg catattaata ccataactac tgaacaacat aaaggtgtca
    35341 tccagatatt cgtcgatctg ctcaatgagc ttattgtctt cattatactt aattttgtaa
    35401 ttaagtgcga aggtcgcaat atgctcaatc agttcattca actggaggtt gatcgccgag
    35461 gttgggtcgt taacccagcc atgattgctt tcttcaaggt ttgcaaggca gtcatgatac
    35521 agggtttcac agagaaactt aagctgtgcg atatcatgtc ttttgggtga gtattcatcc
    35581 ataacgcgtc cccttcttag cggttgaact aacggacacc tttcgggatg gaaaaaactt
    35641 actgacctgg acttgccctc tcgtttgtta gcttaactat aagccactct ttgtaggttt
    35701 tcatcgcatt attacgaaaa attacaatta ggaaggaagc gggacaggac tcactttttc
    35761 atactagcac caacccgcag caggtaaaag cagtttgctc gaactgttca taacatttca
    35821 gaattatcgc cagaagaaat tggtgacact aataccagga ttgctctgaa tatgacgtaa
    35881 taaccgagga atgaataaag aattaccgca aataattaag aatagcctct aaatggttat
    35941 ggattattaa at
//
LOCUS       DADSYM010000028        35929 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2415
            SAMN08039579-rid11723173.denovo.028, whole genome shotgun sequence.
ACCESSION   DADSYM010000028 DADSYM010000000
VERSION     DADSYM010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039579
            Sequence Read Archive: SRR6362533
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 35929)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35929)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980162.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 03:39:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,484
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 4,347
            CDSs (with protein)               :: 4,347
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 43 of 85
            Pseudo Genes (incomplete)         :: 37 of 85
            Pseudo Genes (internal stop)      :: 19 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35929
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039579-rid11723173.denovo.028"
                     /strain="LGE2415"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8302 N 95.9628 W"
                     /collection_date="2015-08-24"
     gene            347..1690
                     /gene="argG"
                     /locus_tag="J1Y83_001171"
     CDS             347..1690
                     /gene="argG"
                     /locus_tag="J1Y83_001171"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HBA7629338.1"
                     /translation="MTTILKHLPVGQRIGIAFSGGLDTSAALLWMRQKGAVPYAYTAN
                     LGQPDEEDYDAIPRRAMEYGAENARLIDCRKQLVAEGIAAIQCGAFHNTTGGLTYFNT
                     TPLGRAVTGTMLVAAMKEDGVNIWGDGSTYKGNDIERFYRYGLLTNAELQIYKPWLDT
                     DFIDELGGRHEMSEFMIACGFDYKMSVEKAYSTDSNMLGATHEAKDLEYLNSSVKIVN
                     PIMGVKFWDESVKIPAEEVTVRFEQGHPVALNGKTFSDDVEMMLEANRIGGRHGLGMS
                     DQIENRIIEAKSRGIYEAPGMALLHIAYERLLTGIHNEDTIEQYHAHGRQLGRLLYQG
                     RWFDSQALMLRDSLQRWVASQITGEVTLELRRGNDYSILNTVSENLTYKPERLTMEKG
                     DSVFSPDDRIGQLTMRNLDITDTREKLFGYAKTGLLSSSAASGVPQVENLENKGQ"
     gene            complement(1698..3323)
                     /locus_tag="J1Y83_001172"
     CDS             complement(1698..3323)
                     /locus_tag="J1Y83_001172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417641.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase"
                     /protein_id="HBA7629339.1"
                     /translation="MTVFNKFVKSFKSHWLLYLCVIVFGITNLVASSGAHMVQRLLFF
                     VLTILVVKRISSLPLRLLIAVPFVLLTAADMSISLYSWCIFGTTFNDGFAISVLQSDP
                     DEVVKMLGMYIPYLCAFAFLSLLFFAVIIKYDVSLPTKKVTGILLLIVISGSLFSACQ
                     FAYKDAKNKEVFSPYILASRFATYTPFFNLNYFALAAKEHQRLLSIANTVPYFQLSVR
                     DTGIDTYVLIVGESVRVDNMSLYGYTRSTTPQVEAQRKQIKLFNQAISGAPYTALSVP
                     LSLTADSVLSHDIHNYPDNIINMANQAGFQTFWLSSQSAFRQNGTAVTSIAMRAMETV
                     YVRGFDELLLPHLSQALQQKTQQKKLIVLHLNGSHEPACSAYPQSSAVFQPQDDQDAC
                     YDNSIHYTDSLLGQVFELLKDRRASVMYFADHGLERDPTKKNVYFHGGREASQQAYHV
                     PMFIWYSPVLGDGVDRTTENNIFSTAYNNYLINAWMGVTKPEQPQTLEEVIAHYKGDS
                     RVVDANHDVFDYVMLRKEFTEDNQGNPTPEGQG"
     gene            complement(3780..3866)
                     /locus_tag="J1Y83_001173"
     tRNA            complement(3780..3866)
                     /locus_tag="J1Y83_001173"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3830..3832),aa:Leu,seq:gag)
     gene            complement(3881..4213)
                     /gene="secG"
                     /locus_tag="J1Y83_001174"
     CDS             complement(3881..4213)
                     /gene="secG"
                     /locus_tag="J1Y83_001174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HBA7629340.1"
                     /translation="MYEALLVVFLIVAIGLVGLIMLQQGKGADMGASFGAGASATLFG
                     SSGSGNFMTRMTALLATLFFIISLVLGNINSNKTNKGSEWENLSAPAKTEQTQPAAPA
                     KPTSDIPN"
     gene            complement(4441..5778)
                     /gene="glmM"
                     /locus_tag="J1Y83_001175"
     CDS             complement(4441..5778)
                     /gene="glmM"
                     /locus_tag="J1Y83_001175"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003905541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HBA7629341.1"
                     /translation="MSNRKYFGTDGIRGRVGDAPITPDFVLKLGWAAGKVLARHGSRK
                     IIIGKDTRISGYMLESALEAGLAAAGLSALFTGPMPTPAVAYLTRTFRAEAGIVISAS
                     HNPFYDNGIKFFSIDGTKLPDAVEEAIEAEMEKEISCVDSAELGKASRIVDAAGRYIE
                     FCKATFPNELSLSELKIVVDCANGATYHIAPNVLRELGANVIAIGCEPNGVNINAEVG
                     ATDVRALQARVLAEKADLGIAFDGDGDRVIMVDHEGNKVDGDQIMYIIAREGLRQGQL
                     RGGAVGTLMSNMGLELALKQLGIPFARAKVGDRYVLEKMQEKGWRIGAENSGHVILLD
                     KTTTGDGIVAGLQVLAAMARNHMSLHDLCSGMKMFPQILVNVRYTAGSGDPLEHESVK
                     AVTAEVEAALGSRGRVLLRKSGTEPLIRVMVEGEDEAQVTEFAHRIADAVKAV"
     gene            complement(5771..6619)
                     /gene="folP"
                     /locus_tag="J1Y83_001176"
     CDS             complement(5771..6619)
                     /gene="folP"
                     /locus_tag="J1Y83_001176"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312083.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HBA7629342.1"
                     /translation="MKLFAQGTSLDLSHPHVMGILNVTPDSFSDGGTHNSLLDAVKHA
                     NLMINAGATIIDVGGESTRPGAAEVSVEEELQRVIPVVEAIAQRFEVWISVDTSKPEV
                     IRESAKVGAHIINDIRSLSEPGALEAAAETGLPVCLMHMQGNPKTMQEAPKYDDVFAE
                     VNRYFIEQIARCEQAGIAKEKLLLDPGFGFGKNLSHNYSLLARLAEFHHFNLPLLVGM
                     SRKSMIGQLLNVGPSERLSGSLACAVIAAMQGAHIIRVHDVKETVEAMRVVEATLSAK
                     ENKRYE"
     gene            complement(6709..8643)
                     /gene="ftsH"
                     /locus_tag="J1Y83_001177"
     CDS             complement(6709..8643)
                     /gene="ftsH"
                     /locus_tag="J1Y83_001177"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HBA7629343.1"
                     /translation="MAKNLILWLVIAVVLMSVFQSFGPSESNGRKVDYSTFLQEVNND
                     QVREARINGREINVTKKDSNRYTTYIPVQDPKLLDNLLTKNVKVVGEPPEEPSLLASI
                     FISWFPMLLLIGVWIFFMRQMQGGGGKGAMSFGKSKARMLTEDQIKTTFADVAGCDEA
                     KEEVAELVEYLREPSRFQKLGGKIPKGVLMVGPPGTGKTLLAKAIAGEAKVPFFTISG
                     SDFVEMFVGVGASRVRDMFEQAKKAAPCIIFIDEIDAVGRQRGAGLGGGHDEREQTLN
                     QMLVEMDGFEGNEGIIVIAATNRPDVLDPALLRPGRFDRQVVVGLPDVRGREQILKVH
                     MRRVPLAPDIDAAIIARGTPGFSGADLANLVNEAALFAARGNKRVVSMVEFEKAKDKI
                     MMGAERRSMVMTEAQKESTAYHEAGHAIIGRLVPEHDPVHKVTIIPRGRALGVTFFLP
                     EGDAISASRQKLESQISTLYGGRLAEEIIYGPEHVSTGASNDIKVATNLARNMVTQWG
                     FSEKLGPLLYAEEEGEVFLGRSVAKAKHMSDETARIIDQEVKALIERNYNRARQLLTD
                     NMDILHAMKDALMKYETIDAPQIDDLMARRDVRPPAGWEEPGASNNSGDNGSPKAPRP
                     VDEPRTPNPGNTMSEQLGDK"
     gene            complement(8743..9372)
                     /gene="rlmE"
                     /locus_tag="J1Y83_001178"
     CDS             complement(8743..9372)
                     /gene="rlmE"
                     /locus_tag="J1Y83_001178"
                     /EC_number="2.1.1.166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uridine(2552)-2'-O)-methyltransferase
                     RlmE"
                     /protein_id="HBA7629344.1"
                     /translation="MTGKKRSASSSRWLQEHFSDKYVQQAQKKGLRSRAWFKLDEIQQ
                     SDKLFKPGMTVVDLGAAPGGWSQYVVTQIGGKGRIIACDLLPMDPIVGVDFLQGDFRD
                     ELVMKALLERVGDSKVQVVMSDMAPNMSGTPAVDIPRAMYLVELALEMCRDVLAPGGS
                     FVVKVFQGEGFDEYLREIRSLFTKVKVRKPDSSRARSREVYIVATGRKP"
     gene            9498..9791
                     /gene="yhbY"
                     /locus_tag="J1Y83_001179"
     CDS             9498..9791
                     /gene="yhbY"
                     /locus_tag="J1Y83_001179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014833438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HBA7629345.1"
                     /translation="MNLSTKQKQHLKGLAHPLKPVVLLGSNGLTEGVLAEIEQALEHH
                     ELIKVKIATEDRETKTLIVEAIVRETGACNVQVIGKTLVLYRPTKERKISLPR"
     gene            complement(9947..10423)
                     /gene="greA"
                     /locus_tag="J1Y83_001180"
     CDS             complement(9947..10423)
                     /gene="greA"
                     /locus_tag="J1Y83_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006178768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HBA7629346.1"
                     /translation="MQAIPMTLRGAEKLREELDFLKSVRRPEIIAAIAEAREHGDLKE
                     NAEYHAAREQQGFCEGRIKDIEAKLSNAQVIDVTKMPNNGRVIFGATVTVLNLDSDEE
                     QTYRIVGDDEADFKQNLISVNSPIARGLIGKEEDDVVVIKTPGGEVEFEVIKVEYL"
     gene            10671..12104
                     /gene="dacB"
                     /locus_tag="J1Y83_001181"
     CDS             10671..12104
                     /gene="dacB"
                     /locus_tag="J1Y83_001181"
                     /EC_number="3.4.16.4"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001212660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase"
                     /protein_id="HBA7629347.1"
                     /translation="MRFSRFIIGLTSCIAFSVQAANVDEYITQLPAGANLALMVQKVG
                     ASAPAIDYHSQQMALPASTQKVITALAALIQLGPDFRFTTTLETKGNVENGILKGDLV
                     ARFGADPTLKRQDIRNMVTTLKKSGVNQIDGNVLIDTSIFASHDKAPGWPWNDMTQCF
                     SAPPAAAIVDRNCFSVSLYSAPKPGDMAFIRVASYYPVTMFSQVRTLPRGSAEAQYCE
                     LDVVPGDLNRFTLTGCLPQRSEPLPLAFAVQDGASYAGAILKDELKQAGITWSGTLLR
                     QTQVNEPGTVVASKQSAPLHDLLKIMLKKSDNMIADTVFRMIGHARFNVPGTWRAGSD
                     AVRQILRQQAGVDIGNTIIADGSGLSRHNLIAPATMMQVLQYIAQHDNELNFISMLPL
                     AGYDGSLQYRAGLHQAGVDGKVSAKTGSLQGVYNLAGFITTASGQRMAFVQYLSGYAV
                     EPADQRNRRIPLVRFESRLYKDIYQNN"
     gene            complement(12145..13317)
                     /gene="cgtA"
                     /locus_tag="J1Y83_001182"
     CDS             complement(12145..13317)
                     /gene="cgtA"
                     /locus_tag="J1Y83_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Obg family GTPase CgtA"
                     /protein_id="HBA7629348.1"
                     /translation="MKFVDEASILVVAGDGGNGCVSFRREKYIPKGGPDGGDGGDGGD
                     VWMEADENLNTLIDYRFEKSFRAERGQNGASRDCTGKRGKDVTIKVPVGTRVIDQGTG
                     ETMGDMTKHGQRLLVAKGGWHGLGNTRFKSSVNRTPRQKTNGTPGDKRELLLELMLLA
                     DVGMLGMPNAGKSTFIRAVSAAKPKVADYPFTTLVPSLGVVRMDNEKSFVVADIPGLI
                     EGAAEGAGLGIRFLKHLERCRVLLHLIDIDPIDGTDPVENARIIIRELEKYSQDLAAK
                     PRWLVFNKIDLLDKAEAEEKAKAIAEALGWEDKYYLISAASGLGVKDLCWDVMTFIIE
                     NPVVQAEEAKQPEKVEFMWDDYHRQQLEEIAEEDDEDWDDDWDEDDEEGVEFIYKR"
     gene            complement(13333..14298)
                     /locus_tag="J1Y83_001183"
     CDS             complement(13333..14298)
                     /locus_tag="J1Y83_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012135318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HBA7629349.1"
                     /translation="MKQQAGIGILLALTTAICWGALPIAMKQVLEVMEPPTIVFYRFL
                     MASIGLGAILAVKKRLPPLRVFRKPRWLILLAVATAGLFGNFILFSSSLQYLSPTASQ
                     VIGQLSPVGMMVASVFILKEKMRSTQVVGALMLLSGLVMFFNTSLVEIFTKLTDYTWG
                     VIFGVGAATVWVSYGVAQKVLLRRLASPQILFLLYTLCTIALFPLADPGVIAQLSHWQ
                     LACLIFCGLNTLVGYGALAEAMARWQAAQVSAIITLTPLFTLFFSDLLSLAWPDFFAR
                     PMLNLLGYLGAFVVVAGAMYSAIGHRIWGGLRKHTTVVSQPRAGE"
     gene            complement(14425..14682)
                     /gene="rpmA"
                     /locus_tag="J1Y83_001184"
     CDS             complement(14425..14682)
                     /gene="rpmA"
                     /locus_tag="J1Y83_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007369914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HBA7629350.1"
                     /translation="MAHKKAGGSTRNGRDSEAKRLGVKRFGGESVLAGSIIVRQRGTK
                     FHAGANVGCGRDHTLFAKADGKVKFEVKGPKNRKFISIEAE"
     gene            complement(14703..15014)
                     /gene="rplU"
                     /locus_tag="J1Y83_001185"
     CDS             complement(14703..15014)
                     /gene="rplU"
                     /locus_tag="J1Y83_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015960603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HBA7629351.1"
                     /translation="MYAVFQSGGKQHRVSEGQTVRLEKLDIATGETVEFAEVLMIANG
                     EEVKIGVPFVDGGVIKAEVVAHGRGEKVKIVKFRRRKHYRKQQGHRQWFTDVKITGIS
                     A"
     gene            15273..16244
                     /gene="ispB"
                     /locus_tag="J1Y83_001186"
     CDS             15273..16244
                     /gene="ispB"
                     /locus_tag="J1Y83_001186"
                     /EC_number="2.5.1.90"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="octaprenyl diphosphate synthase"
                     /protein_id="HBA7629352.1"
                     /translation="MNLEKINELTAQDMAGVNAAILEQLNSDVQLINQLGYYIVSGGG
                     KRIRPMIAVLAARAVGYEGNAHVTIAALIEFIHTATLLHDDVVDESDMRRGKATANAA
                     FGNAASVLVGDFIYTRAFQMMTSLGSLKVLEVMSEAVNVIAEGEVLQLMNVNDPDITE
                     ENYMRVIYSKTARLFEAAAQCSGILAGCTPEEEKGLQDYGRYLGTAFQLIDDLLDYNA
                     DGEQLGKNVGDDLNEGKPTLPLLHAMHHGTPEQAQMIRTAIEQGNGRHLLEPVLEAMN
                     ACGSLEWTRQRAEEEADKAIAALQVLPDTPWREALVGLAHIAVQRDR"
     gene            16477..16755
                     /gene="sfsB"
                     /locus_tag="J1Y83_001187"
     CDS             16477..16755
                     /gene="sfsB"
                     /locus_tag="J1Y83_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SfsB"
                     /protein_id="HBA7629353.1"
                     /translation="MESNFIDWHPADIIAGLRKKGTSMAAESRRNGLSSSTLANALSR
                     PWPKGEMIIAKALGTDPWVIWPSRYHDPQTHEFIDRTQLMRSYTKPKK"
     gene            complement(16803..18062)
                     /gene="murA"
                     /locus_tag="J1Y83_001188"
     CDS             complement(16803..18062)
                     /gene="murA"
                     /locus_tag="J1Y83_001188"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HBA7629354.1"
                     /translation="MDKFRVQGPTKLQGEVTISGAKNAALPILFAALLAEEPVEIQNV
                     PKLKDVDTSMKLLSQLGAKVERNGSVHIDARDVNVFCAPYDLVKTMRASIWALGPLVA
                     RFGQGQVSLPGGCTIGARPVDLHISGLEQLGATIKLEEGYVKASVDGRLKGAHIVMDK
                     VSVGATVTIMCAATLAEGTTIIENAAREPEIVDTANFLITLGAKISGQGTDRIVIEGV
                     ERLGGGVYRVLPDRIETGTFLVAAAISRGKIICRNAQPDTLDAVLAKLRDAGADIEVG
                     EDWISLDMHGKRPKAVNVRTAPHPAFPTDMQAQFTLLNLVAEGTGFITETVFENRFMH
                     VPELSRMGAHAEIESNTVICHGVEKLSGAQVMATDLRASASLVLAGCIAEGTTVVDRI
                     YHIDRGYERIEDKLRALGANIERVKGE"
     gene            complement(18117..18371)
                     /gene="ibaG"
                     /locus_tag="J1Y83_001189"
     CDS             complement(18117..18371)
                     /gene="ibaG"
                     /locus_tag="J1Y83_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015585657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA family iron metabolism protein IbaG"
                     /protein_id="HBA7629355.1"
                     /translation="MENNEIQSVLMNALSLQEVHVSGDGSHFQVIAVGELFDGMSRVK
                     KQQTVYGPLMEYIADNRIHAVSIKAYTPAEWARDRKLNGF"
     gene            complement(18531..18824)
                     /gene="mlaB"
                     /locus_tag="J1Y83_001190"
     CDS             complement(18531..18824)
                     /gene="mlaB"
                     /locus_tag="J1Y83_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance protein MlaB"
                     /protein_id="HBA7629356.1"
                     /translation="MSESLSWMQTGDTLALSGELDQDVLLPLWEMREEAVKGITCIDL
                     SRVSRVDTGGLALLLHLIDLAKKQGNNVTLQGVNDKVYTLAKLYNLPADVLPR"
     gene            complement(18824..19459)
                     /gene="mlaC"
                     /locus_tag="J1Y83_001191"
     CDS             complement(18824..19459)
                     /gene="mlaC"
                     /locus_tag="J1Y83_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid-binding protein MlaC"
                     /protein_id="HBA7629357.1"
                     /translation="MFKRLMMVALLVIAPLSAATAADQTNPYKLMDEAAQKTFDRLKN
                     EQPQIRANPDYLRTIVDQELLPYVQVKYAGALVLGQYYKSATPAQRDAYFAAFREYLK
                     QAYGQALAMYHGQTYQIAPEQPLGDKTIVPIRVTIIDPNGRPPVRLDFQWRKNSQTGN
                     WQAYDMIAEGVSMITTKQNEWGTLLRTKGIDGLTAQLKSISQQKITLEEKK"
     gene            complement(19478..20029)
                     /gene="mlaD"
                     /locus_tag="J1Y83_001192"
     CDS             complement(19478..20029)
                     /gene="mlaD"
                     /locus_tag="J1Y83_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipid asymmetry maintenance
                     protein MlaD"
                     /protein_id="HBA7629358.1"
                     /translation="MQTKKNEIWVGIFLLAALLAALFVCLKAANVTSIRTEPTYTLYA
                     TFDNIGGLKARSPVSIGGVVVGRVADITLDPKTYLPRVTLEIEQRYNHIPDTSSLSIR
                     TSGLLGEQYLALNVGFEDPELGTAILKDGDTIQDTKSAMVLEDLIGQFLYGSKGDDNK
                     NSGDAPAAAPGNNETTEPVGTTK"
     gene            complement(20034..20816)
                     /gene="mlaE"
                     /locus_tag="J1Y83_001193"
     CDS             complement(20034..20816)
                     /gene="mlaE"
                     /locus_tag="J1Y83_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014071945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance ABC transporter
                     permease subunit MlaE"
                     /protein_id="HBA7629359.1"
                     /translation="MLLNALASLGHKGIKTLRTFGRAGLMLFNALVGKPEFRKHAPLL
                     VRQLYNVGVLSMLIIVVSGVFIGMVLGLQGYLVLTTYSAETSLGMLVALSLLRELGPV
                     VAALLFAGRAGSALTAEIGLMRATEQLSSMEMMAVDPLRRVISPRFWAGVISLPLLTV
                     IFVAVGIWGGSLVGVSWKGIDSGFFWSAMQNAVDWRMDLVNCLIKSVVFAITVTWISL
                     FNGYDAIPTSAGISRATTRTVVHSSLAVLGLDFVLTALMFGN"
     gene            complement(20824..21633)
                     /gene="mlaF"
                     /locus_tag="J1Y83_001194"
     CDS             complement(20824..21633)
                     /gene="mlaF"
                     /locus_tag="J1Y83_001194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid ABC transporter ATP-binding protein
                     MlaF"
                     /protein_id="HBA7629360.1"
                     /translation="MEQSVANLVDMRDVSFTRGNRCIFDNISLTVPRGKITAIMGPSG
                     IGKTTLLRLIGGQIAPDHGEILFDGENIPAMSRSRLYTVRKRMSMLFQSGALFTDMNV
                     FDNVAYPLREHTQLPAPLLHSTVMMKLEAVGLRGAAKLMPSELSGGMARRAALARAIA
                     LEPDLIMFDEPFVGQDPITMGVLVKLISELNSALGVTCVVVSHDVPEVLSIADHAWIL
                     ADKKIVAHGSAQALQANPDPRVRQFLDGIADGPVPFRYPAGDYHADLLPGS"
     gene            21843..22820
                     /locus_tag="J1Y83_001195"
     CDS             21843..22820
                     /locus_tag="J1Y83_001195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium/sodium antiporter"
                     /protein_id="HBA7629361.1"
                     /translation="MLLATALLIVGLLLVVYSADRLVFAASILCRTFGIPPLIIGMTV
                     VSIGTSLPEIIVSLAASLHEQRDLAVGTALGSNIINILLILGLAALVRPFTVHSDVLR
                     RELPLMLLVSVVAGSVLYDGQLSRSDGIFLLFLAVLWLLFIVKLARQAERQGTDSLTR
                     EQLAELPREGGLPVAFLWLGIALIIMPVATRMVVDNATVLANYFAISELTMGLTAIAI
                     GTSLPELATAIAGVRKGENDIAVGNIIGANIFNIVIVLGLPALITPGEIDPLAYSRDY
                     SVMLLVSIIFALLCWRRSPQPGRGVGVLLTGGFIVWLAMLYWLSPILVE"
     gene            22834..23820
                     /gene="kdsD"
                     /locus_tag="J1Y83_001196"
     CDS             22834..23820
                     /gene="kdsD"
                     /locus_tag="J1Y83_001196"
                     /EC_number="5.3.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-5-phosphate isomerase KdsD"
                     /protein_id="HBA7629362.1"
                     /translation="MSHVELQPGFDFQQAGKEVLAIERECLAELDQYINQNFTLACEK
                     MFWCKGKVVVMGMGKSGHIGRKMAATFASTGTPSFFVHPGEAAHGDLGMVTPQDVVIA
                     ISNSGESSEITALIPVLKRLHVPLICITGRPESSMARAADVHLCVKVAKEACPLGLAP
                     TSSTTATLVMGDALAVALLKARGFTAEDFALSHPGGALGRKLLLRVNDIMHTGDEIPH
                     VKKTASLRDALLEVTRKNLGMTVICDDNMMIEGIFTDGDLRRVFDMGVDVRQLSIADV
                     MTPGGIRVRPGILAVEALNLMQSRHITSVMVADGDHLLGVLHMHDLLRAGVV"
     gene            23841..24407
                     /gene="kdsC"
                     /locus_tag="J1Y83_001197"
     CDS             23841..24407
                     /gene="kdsC"
                     /locus_tag="J1Y83_001197"
                     /EC_number="3.1.3.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate-8-phosphatase KdsC"
                     /protein_id="HBA7629363.1"
                     /translation="MSKAGASLATCYGPVSADVMAKAENIRLLILDVDGVLSDGLIYM
                     GNNGEELKAFNVRDGYGIRCALTSDIEVAIITGRKAKLVEDRCATLGITHLYQGQSNK
                     LIAFSDLLEKLAIAPENVAYVGDDLIDWPVMEKVGLSVAVADAHPLLIPRADYVTRIA
                     GGRGAVREVCDLLLLAQGKLDEAKGQSI"
     gene            24404..24979
                     /gene="lptC"
                     /locus_tag="J1Y83_001198"
     CDS             24404..24979
                     /gene="lptC"
                     /locus_tag="J1Y83_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HBA7629364.1"
                     /translation="MSKARRWVIIVLSLAVLVMIGINMAEKDDTAQVVVNNNDPTYKS
                     EHTDTLVYNPEGALSYRLIAQHVEYYSDQAVSWFTQPVLTTFDKDKIPTWSVKADKAK
                     LTNDRMLYLYGHVEVNALVPDSQLRRITTDNAQINLVTQDVTSEDLVTLYGTTFNSSG
                     LKMRGNLRSKNAELIEKVRTSYEIQNKQTQP"
     gene            24948..25505
                     /gene="lptA"
                     /locus_tag="J1Y83_001199"
     CDS             24948..25505
                     /gene="lptA"
                     /locus_tag="J1Y83_001199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide ABC transporter
                     substrate-binding protein LptA"
                     /protein_id="HBA7629365.1"
                     /translation="MKFKTNKLSLNLVLASSLLAASIPAFAVTGDTDQPIHIESDQQS
                     LDMQGNVVTFTGNVIVTQGTIKINADKVVVTRPGGEQGKEVIDGYGKPATFYQMQDNG
                     KPVEGHASQMHYELAKDFVVLTGNAYLQQVDSNIKGDKITYLVKEQKMQAFSDKGKRV
                     TTVLVPSQLQDKNNKGQTPAQKKGN"
     gene            25512..26237
                     /gene="lptB"
                     /locus_tag="J1Y83_001200"
     CDS             25512..26237
                     /gene="lptB"
                     /locus_tag="J1Y83_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004091254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HBA7629366.1"
                     /translation="MATLTAKNLAKAYKGRRVVEDVSLTVNSGEIVGLLGPNGAGKTT
                     TFYMVVGIVPRDAGNIIIDDDDISLLPLHARARRGIGYLPQEASIFRRLSVYDNLMAV
                     LQIRDDLSAEQREDRANELMEEFHIEHLRDSMGQSLSGGERRRVEIARALAANPKFIL
                     LDEPFAGVDPISVIDIKRIIEHLRDSGLGVLITDHNVRETLAVCERAYIVSQGHLIAH
                     GTPTEILQDEHVKRVYLGEDFRL"
     gene            26285..27718
                     /gene="rpoN"
                     /locus_tag="J1Y83_001201"
     CDS             26285..27718
                     /gene="rpoN"
                     /locus_tag="J1Y83_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HBA7629367.1"
                     /translation="MKQGLQLRLSQQLAMTPQLQQAIRLLQLSTLELQQELQQALESN
                     PLLEQIDTHEEIDTRETQDSETLDTADALEQKEMPEELPLDASWDTIYTAGTPSGTSG
                     DYIDDELPVYQGETTQTLQDYLMWQVELTPFSDTDRAIATSIVDAVDDTGYLTVPLED
                     ILESMGDEEIDIDEVEAVLKRIQRFDPVGVAAKDLRDCLLIQLSQFDKTTPWLEEARL
                     IISDHLDLLANHDFRTLMRVTRLKEDVLKEAVNLIQSLDPRPGQSIQTGEPEYVIPDV
                     LVRKHNGHWTVELNSDSIPRLQINQHYASMCNNARNDGDSQFIRSNLQDAKWLIKSLE
                     SRNDTLLRVSRCIVEQQQAFFEQGEEYMKPMVLADIAQAVEMHESTISRVTTQKYLHS
                     PRGIFELKYFFSSHVNTEGGGEASSTAIRALVKKLIAAENPAKPLSDSKLTSLLSEQG
                     IMVARRTVAKYRESLSIPPSNQRKQLV"
     gene            27741..28028
                     /gene="hpf"
                     /locus_tag="J1Y83_001202"
     CDS             27741..28028
                     /gene="hpf"
                     /locus_tag="J1Y83_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007369896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome hibernation promoting factor"
                     /protein_id="HBA7629368.1"
                     /translation="MQLNITGNNVEITEALREFVTAKFAKLEQYFDRINQVYVVLKVE
                     KVTHTSDATLHVNGGEIHASAEGQDMYAAIDGLIDKLARQLTKHKDKLKQH"
     gene            28146..28637
                     /gene="ptsN"
                     /locus_tag="J1Y83_001203"
     CDS             28146..28637
                     /gene="ptsN"
                     /locus_tag="J1Y83_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS IIA-like nitrogen regulatory protein PtsN"
                     /protein_id="HBA7629369.1"
                     /translation="MTNNDTTLQLSSVLNRECTRSRVHCQSKKRALEIISELAAKQLS
                     LAPQVVFEAILTREKMGSTGIGNGIAIPHGKLEEDTLRAVGVFVQLETPIAFDAIDNQ
                     PVDLLFALLVPADQTKTHLHTLSLVAKRLADKTICRRLRAAQSDEELYQIITDTEGTP
                     DEA"
     gene            28683..29537
                     /gene="rapZ"
                     /locus_tag="J1Y83_001204"
     CDS             28683..29537
                     /gene="rapZ"
                     /locus_tag="J1Y83_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HBA7629370.1"
                     /translation="MVLMIVSGRSGSGKSVALRALEDMGFYCVDNLPVVLLPDLARTL
                     ADREISAAVSIDVRNMPESPEIFEQAMSNLPDAFSPQLLFLDADRNTLIRRYSDTRRL
                     HPLSSKNLSLESAIDKESDLLEPLRSRADLIVDTSEMSVHELAEMLRTRLLGKREREL
                     TMVFESFGFKHGIPIDADYVFDVRFLPNPHWDPKLRPMTGLDKPVAAFLDRHTEVHNF
                     IYQTRSYLELWLPMLETNNRSYLTVAIGCTGGKHRSVYIAEQLADYFRSRGKNVQSRH
                     RTLEKRKP"
     gene            29534..29806
                     /gene="npr"
                     /locus_tag="J1Y83_001205"
     CDS             29534..29806
                     /gene="npr"
                     /locus_tag="J1Y83_001205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000216792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS phosphocarrier protein NPr"
                     /protein_id="HBA7629371.1"
                     /translation="MTVKQTVEITNKLGMHARPAMKLFELMQGFDAEVLLRNDEGTEA
                     EANSVIALLMLDSAKGRQIEVEATGPQEEEALAAVIALFNSGFDED"
     gene            30020..30652
                     /gene="yrbL"
                     /locus_tag="J1Y83_001206"
     CDS             30020..30652
                     /gene="yrbL"
                     /locus_tag="J1Y83_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoP regulatory network protein YrbL"
                     /protein_id="HBA7629372.1"
                     /translation="MIRLSEQSPLGTGRHRKCYAHPEDAQRCIKIVYHRGDGGDKEIR
                     RELKYYAHLGRRLKDWSGIPRYHGTVETDCGTGYVYDVIADFDGKPSITLTEFAEQCR
                     YEEDIAQLRQLLKQLKRYLQDNRIVTMSLKPQNILCHRISESEVTPVVCDNIGESTLI
                     PLATWSKWCCLRKQERLWKRFIAQPALAIALQKDLQPRESKTLALTSREA"
     gene            complement(30649..31377)
                     /gene="mtgA"
                     /locus_tag="J1Y83_001207"
     CDS             complement(30649..31377)
                     /gene="mtgA"
                     /locus_tag="J1Y83_001207"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monofunctional biosynthetic peptidoglycan
                     transglycosylase"
                     /protein_id="HBA7629373.1"
                     /translation="MSKSRLTVFSFVRRFLLRLMVVLAIFWGGGIALFSVAPVPFSAV
                     MVERQVSAWLHGNFRYVAHSDWVSMDQISPWMGLAVIAAEDQKFPEHWGFDVASIEQA
                     LAHNERNENRIRGASTISQQTAKNLFLWDGRSWVRKGLEAGLTLGIETVWSKKRILTV
                     YLNIAEFGDGVFGVEAAAQRYFHKPASKLTRSEAALLAAVLPNPLRFKVSAPSGYVRS
                     RQAWILRQMYQLGGEPFMQQHQLD"
     gene            complement(31374..32027)
                     /gene="elbB"
                     /locus_tag="J1Y83_001208"
     CDS             complement(31374..32027)
                     /gene="elbB"
                     /locus_tag="J1Y83_001208"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenoid biosynthesis glyoxalase ElbB"
                     /protein_id="HBA7629374.1"
                     /translation="MKKIGVILSGCGVYDGSEIHEAVLTLLAISRSGAQAVCFAPDKQ
                     QVDVINHLTGEAMTETRNVLIEAARITRGEIRPLAQADAAELDALIVPGGFGAAKNLS
                     NFASLGSECTVDRELKALAQAMHQAGKPLGFMCIAPAILPKIFDFPLRLTIGTDIDTA
                     EVLEEMGAEHVPCPVDDIVVDEDNKIVTTPAYMLAQNIAEAASGIDKLVSRVLVLAE"
     gene            complement(32257..34593)
                     /gene="arcB"
                     /locus_tag="J1Y83_001209"
     CDS             complement(32257..34593)
                     /gene="arcB"
                     /locus_tag="J1Y83_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerobic respiration two-component sensor
                     histidine kinase ArcB"
                     /protein_id="HBA7629375.1"
                     /translation="MKQIRLLAQYYVDLMMKLGLVRFSMLLALALVVLAIVVQMAVTM
                     VLHGQVESIDVIRSIFFGLLITPWAVYFLSVVVEQLEESRQRLSRLVQKLEEMRERDL
                     SLNVQLKDNIAQLNQEIAVREKAEAELQETFGQLKIEIKEREETQIQLEQQSSFLRSF
                     LDASPDLVFYRNEDKEFSGCNRAMELLTGKSEKQLVHLKPADVYSPEAAAKVIETDEK
                     VFRHNVSLTYEQWLDYPDGRKACFEIRKVPYYDRVGKRHGLMGFGRDITERKRYQDAL
                     ERASRDKTTFISTISHELRTPLNGIVGLSRILLDTELTAEQEKYLKTIHVSAVTLGNI
                     FNDIIDMDKMERRKVQLDNQPVDFTSFLADLENLSALQAQQKGLRFNLEPTLPLPHQV
                     ITDGTRLRQILWNLISNAVKFTQQGQVTVRVRYDEGDMLHFEVEDSGIGIPQDELDKI
                     FAMYYQVKDSHGGKPATGTGIGLAVSRRLAKNMGGDITVTSEQGKGSTFTLTIHAPSV
                     AEEVDDAFDEDDMPLPALNVLLVEDIELNVIVARSVLEKLGNSVDVAMTGKAALEMFK
                     PGEYDLVLLDIQLPDMTGLDISRELTKRYPREDLPPLVALTANVLKDKQEYLNAGMDD
                     VLSKPLSVPALTAMIKKFWDTQDDEESTVTTEENSKSEALLDIPMLEQYLELVGPKLI
                     TDGLAVFERMMPGYVSVLESNLTAQDKKGIVEEGHKIKGAAGSVGLRHLQQLGQQIQS
                     PDLPAWEDNVGEWIEEMKEEWRHDVEVLKAWVAKATKK"
     gene            complement(34689..35618)
                     /locus_tag="J1Y83_001210"
     CDS             complement(34689..35618)
                     /locus_tag="J1Y83_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562163.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HBA7629376.1"
                     /translation="MQLQKLVNMFGGDLTRRYGQKVHKLTLHGGFSCPNRDGTIGRGG
                     CTFCNVASFADEAQQHRSIAEQLAHQANLVNRAKRYLAYFQAYTSTFAEVQVLRSMYQ
                     QAVSQANIVGLCVGTRPDCVPDAVLDLLCEYKDQGYEVWLELGLQTAHDKTLHRINRG
                     HDFACYQRTTQLARERGLKVCSHLIVGLPGEGQAECLQTLERVVETGVDGIKLHPLHI
                     VKGSIMAKAWEAGRLNGIELEDYTLTAGEMIRHTPPEVIYHRISASARRPTLLAPLWC
                     ENRWTGMVELDRYLNEHGVQGSALGRPWLPPTA"
BASE COUNT         9083 a         9598 c         8608 g         8640 t
ORIGIN      
        1 ctgaaacgtg gcaaattcta ctcgttttgg gtaaaaaatg caaatactgc tgggatttgg
       61 tgtaccgaga cgggacgtaa aatctgcagg cattatagtg atccacgcca cattttgtca
      121 acgtttattg ctaatcacgt gaatgaatat ccagttcact ttcatttgtt gaatactttt
      181 gccttctcct gctctccctt aagcgcatta ttttacaaaa aacacactaa actcttcctg
      241 tctccgataa aagatgatta aatgaaaact catttatttt gcataaaaat tcagtgagag
      301 cggaaatcca ggctcatcat cagttaatta agcagggtgt tattttatga cgacgattct
      361 caagcatctc ccggtaggtc aacgtattgg catcgctttt tccggcggtc tggacaccag
      421 tgccgcactg ctgtggatgc gacaaaaggg agcggttcct tatgcatata ctgcaaacct
      481 gggccagcca gacgaagagg attatgatgc gatccctcgt cgtgccatgg aatacggcgc
      541 ggagaacgca cgtctgatcg actgccgcaa acaactggtg gccgaaggta ttgccgctat
      601 tcagtgtggc gcatttcata acaccactgg tggactgacc tatttcaaca cgacgccgct
      661 gggccgcgcc gtgaccggca ccatgctggt tgctgcgatg aaagaagatg gcgtgaatat
      721 ctggggtgac ggcagcacct ataaaggaaa cgatatcgaa cgtttctacc gttacggtct
      781 gctgaccaat gctgaactgc agatttacaa accgtggctt gatactgact ttattgatga
      841 actgggcggc cgtcatgaga tgtctgaatt tatgattgcc tgcggtttcg actacaaaat
      901 gtctgtcgaa aaagcttact ccacggactc caacatgctt ggtgcaacgc atgaagcgaa
      961 ggatctggaa tacctcaact ccagcgtcaa aatcgtcaat ccaattatgg gcgtgaagtt
     1021 ttgggatgag agcgtgaaaa tcccggcaga agaagtcaca gtacgctttg agcaaggtca
     1081 tccggtggcg ttgaacggta aaacctttag cgacgacgta gaaatgatgc tggaagctaa
     1141 ccgcatcggc ggtcgtcacg gcctgggcat gagcgaccag attgaaaacc gtatcatcga
     1201 agcaaaaagc cgcggcattt atgaagcccc tgggatggca ctgctgcaca ttgcgtatga
     1261 acgtttgttg accggtattc acaacgaaga caccattgag cagtatcacg cgcatggtcg
     1321 tcagttgggc cgtctgctgt accagggacg ttggtttgac tcccaggcgc tgatgctgcg
     1381 ggactctctg caacgctggg ttgccagcca gatcactggt gaagttactc tggagctgcg
     1441 ccgcgggaac gattattcaa tcctgaatac cgtctcagag aacctgacct acaagccaga
     1501 gcgtctgacg atggaaaaag gcgactcggt gttctcgcca gatgatcgta tcggtcaact
     1561 gaccatgcgt aacctggata tcaccgatac ccgcgagaaa cttttcggtt atgccaaaac
     1621 tggcctgctc tcctcctctg ccgcttcagg ggtgccgcag gtggagaatc tggaaaataa
     1681 aggccagtaa ttcgacatca accctgccct tcaggggtgg ggttaccttg attatcctct
     1741 gtaaactcct ttctgagcat cacataatcg aaaacatcat ggtttgcatc cacaacccgc
     1801 gagtctcctt tatagtgtgc aatcacttcc tcaagcgttt gcggctgttc cggctttgtt
     1861 acccccatcc acgcattaat aaggtaatta ttgtaagctg tcgaaaagat gttgttttcc
     1921 gttgtgcgat ccacgccatc cccaagaacg gggctatacc agataaacat agggacatga
     1981 tatgcctgct ggctagcctc cctgcctcca tgaaaataga cgttcttctt cgtagggtcg
     2041 cgttccaggc cgtggtcggc aaaatacatg accgaggcgc ggcgatcttt taataattca
     2101 aaaacctgac ctagcaaact atctgtgtaa tgaatggagt tgtcatagca ggcatcctga
     2161 tcgtcctgcg gttgaaacac agcgctggat tgcggatagg cgctacaagc cggttcatgg
     2221 cttccattta aatgaagaac aatcagtttc ttttgctgcg tcttttgctg taacgcttgc
     2281 gataaatgcg gcaacaacaa ttcatcaaat cctctgacat agactgtttc catggcgcgc
     2341 atggcgatac tggtaactgc tgtaccattc tgccgaaaag cggattgcga gcttagccag
     2401 aaagtctgaa atcctgcttg attagccata ttaataatgt tgtccgggta attatgaatg
     2461 tcatgactca aaacagaatc agcagttaaa gaaaggggaa ccgacagcgc agtgtaaggt
     2521 gcaccgctta ttgcttgatt aaacagtttg atctgctttc tttgtgcttc gacttgcggt
     2581 gtcgtagagc gtgtatatcc atacaaagac atattgtcga cacgtacaga ctcccccaca
     2641 atcaacacgt aggtatcaat acctgtatcc ctgactgata gttgaaaata cggcaccgtg
     2701 tttgcaattg agagtaatct ttgatgctct ttcgctgcta aagcaaaata attgagattg
     2761 aaaaacggcg tataggtagc aaatcgcgac gctagtatat atggactgaa cacctcttta
     2821 ttttttgcat ctttataagc aaattgacaa gcggaaaata aactgcccga aatgacaatc
     2881 agcaataata ttccggtcac tttttttgtc ggcaaggaaa catcatattt tattattact
     2941 gcaaaaaaaa gaagggataa aaaggcaaaa gcacatagat aagggatata cattcccagc
     3001 attttgacaa cttcatccgg atcactctgc agcacactaa tcgcaaatcc atcattgaaa
     3061 gttgtaccaa agatacacca tgaatagagg ctaatactca tatctgccgc agtcagtaac
     3121 acaaatggta cggcaataag caggcgaagc ggaagggatg atatacgttt aacaaccagg
     3181 atggtcagaa cgaagaacag caatcgctga accatatgcg ctccggaaga agcgactaag
     3241 ttcgtaatac caaaaacaat aacacaaaga tacaacaacc aatgagattt aaaactttta
     3301 acaaatttgt tgaatacagt catccagaaa tccaagttca caaccaatat aaacaattat
     3361 caacaatgtt caattttata tcagacatgt ttttcaactc tgatccatat cagaccgaaa
     3421 cactgttaga ttttttcttt ttaaatgatg cggccattgc gttctcagta attaaataac
     3481 attatcaatg cgtctctaaa cattacgcag aacgtagcca tcagtagtta tactgacagg
     3541 ggcaggatgg tttatcgaga agtaaacata tccgtattcg taccggaaat ggagttgaca
     3601 caataaagtg ccaattatgt cagcagaagg gaaaagctta taactaaggc ataccggcac
     3661 catcgtttca aggtaccagc tacgagtaaa acaactggac gagatacaga tacctgacaa
     3721 ccattcctca gaccaggacc aaaacgaaaa aagacgcttt tcagcgtctt tctggaattt
     3781 ggtaccgagg acgggacttg aacccgtaag ccctattggg cactaccacc tcaaggtagc
     3841 gtgtctacca attccaccac ctcggcacgg atactacttt ttagttcggg atatcgctgg
     3901 tcggcttagc cggagcagct ggctgagttt gttcggtttt cgccggcgca ctcaggtttt
     3961 cccattcgct acctttattg gttttgttgc tattgatgtt acccagcacc agactgatga
     4021 tgaagaataa cgttgccagc agcgctgtca tgcgggtcat gaagttacca gaaccacttg
     4081 aaccaaacag cgtagcggaa gcgcctgctc cgaaggaggc tcccatatca gcgcctttac
     4141 cttgctgcag catgatcaga ccaacaaggc caattgccac aataaggaaa actactaaaa
     4201 gagcttcgta cataatcaac ctgttccttg cggagttgcc gcgtaccaat gcttcaacca
     4261 ataaagcggg agtttttaat ctttcccact gaagcgggtg tgaatactaa ccaaagcgaa
     4321 tgaccttcgc aagggcaatt ttattgcatt gtatcaactg cggaaaaaaa cagcaaaacc
     4381 gatgtgttcg ctgagaaaaa aggcgacaat cgccgccttt ttagccagcc atttatcgtt
     4441 ttaaacggct tttactgcat cggcgatgcg gtgtgcaaat tcagtcacct gcgcttcgtc
     4501 ttcgccttcc accatcacgc gaattaacgg ttcggtgccg gatttacgca acagcacacg
     4561 tccacggcta cccagcgcag cttcaacttc tgcggtcaca gctttgactg actcatgctc
     4621 cagcggatcg ccgctgcctg ctgtgtaacg cacgttaacc agaatctgcg ggaacatttt
     4681 catgccgctg caaaggtcgt gcaggctcat atggttacgc gccatcgccg ccagcacctg
     4741 caagccagca acaatgccgt caccggtagt ggttttatcc agcaggatca catgaccgga
     4801 gttctctgca ccgatacgcc agcctttctc ctgcattttt tccagtacgt accggtcacc
     4861 cactttcgcg cgcgcaaatg gaatacctaa ctgtttcagc gccagttcaa gccccatgtt
     4921 gctcatcaat gtacccacag cacctccacg cagctggccc tgacgaagac cctcacgcgc
     4981 gatgatatac atgatctgat cgccatcgac tttattgcct tcatggtcaa ccataatcac
     5041 gcgatcgccg tcgccgtcga aggcaatacc gagatccgct ttttcagcca gcacacgagc
     5101 ctggagcgcg cgaacgtcgg tagcccccac ttcggcattg atatttacac cgttcggctc
     5161 acaaccgata gcgataacgt tcgcccccag ttcacgcagc acgttcggcg cgatgtggta
     5221 agtcgcaccg tttgcacaat ccaccacaat cttcaattca ctgaggctaa gttcgttcgg
     5281 gaacgtagct ttgcaaaact cgatataacg acccgcggca tcaacgatac ggctggcttt
     5341 acccagttct gccgaatcaa cacagctgat ctctttttcc atttccgctt cgatggcttc
     5401 ttcaactgca tccggcagct tagtaccgtc aatagaaaag aatttaatgc cgttatcgta
     5461 gaatgggtta tgcgatgcag aaattacaat tccagcctct gcgcggaagg tacgcgtcag
     5521 ataagccacg gccggtgttg gcatcgggcc agtgaagagc gcggaaagac cggctgctgc
     5581 aagccccgct tccagtgctg actccagcat atagccagaa atacgcgtgt ctttaccaat
     5641 aataatctta cgggagccgt ggcgcgccag cactttaccc gcggcccaac ccagcttaag
     5701 cacaaaatca ggtgtgatcg gcgcatcccc tacacgacca cgaatcccat cggtaccgaa
     5761 atatttacga ttactcatag cgtttgtttt cctttgcaga cagagtggct tccaccaccc
     5821 gcatcgcttc tacggtttct ttgacgtcat gaacacgaat gatgtgcgcg ccttgcattg
     5881 cggcaatgac cgcacaggcc agactaccgc tcaggcgctc ggacggcccc acgttcagca
     5941 attgcccaat catcgatttt cgtgacatac ccaccaacag cggcaggttg aaatggtgaa
     6001 attcagccag gcgcgccagt aatgaatagt tatgggagag atttttaccg aaaccgaatc
     6061 cggggtcgag caacaatttc tcttttgcga tacccgcctg ctcgcaacgt gctatttgct
     6121 caataaagta gcgattcact tctgcaaaga catcgtcata cttcggcgct tcctgcatgg
     6181 tttttggatt tccctgcata tgcatcagac aaaccggtaa accggtttct gcagccgcct
     6241 ccagagcacc aggttcggaa agggagcgga tatcgttaat aatgtgagcg ccaactttcg
     6301 ctgactcacg gatgacttct ggtttggatg tatctaccga gatccagact tcgaagcgtt
     6361 gagcaattgc ctcaaccaca ggaataacac gttgcaactc ttcttcaacg ctaacttccg
     6421 ccgcccctgg gcgcgtggac tcgccaccaa cgtcaatgat cgtcgcgcca gcgttgatca
     6481 tcagattcgc atgtttcacc gcatctaaca gcgagttatg cgtgccacca tccgaaaagg
     6541 aatcaggcgt gacgttgagg atccccatta cgtgaggatg gctaaggtcc agtgaagtac
     6601 cctgggcaaa gagtttcatg atgttatccc tggtatgaat tgataagaaa aaaccccgga
     6661 gcacgccccg gggttttcgg tacaaataca gtcatctgat acgggaactt acttgtcgcc
     6721 taactgctct gacatggtgt tacccgggtt cggcgtacgc ggttcatcaa ccggacgagg
     6781 agcctttgga ctaccattgt cgccagaatt gttagaagcg cctggttctt cccagcccgc
     6841 tggcggacgt acatcgcgac gtgccatcag gtcatcaatc tgcggtgcgt cgatagtctc
     6901 atatttcatg agagcatctt tcatcgcatg cagaatatcc atattgtcgg tcagaagctg
     6961 gcgcgcacga ttatagttac gctcaatcag tgctttcact tcctggtcga tgatacgtgc
     7021 agtttcatcg gacatatgtt tcgctttcgc tacgctacgg ccgaggaaca cttcaccttc
     7081 ttcttccgcg tacagcagtg gacccaattt ctcagagaag ccccactggg tcaccatatt
     7141 acgtgccagg ttggtcgcaa ctttaatgtc gttggacgcc ccggtagaaa catgttccgg
     7201 tccgtagatg atctcttccg ccagacgacc accgtacagc gtggagatct gactttccag
     7261 tttctgacgg ctggcgctga ttgcatcacc ttcaggcaag aagaaggtca cacccagcgc
     7321 acgaccgcgc gggataatcg ttactttgtg caccggatcg tgttccggta ccaggcgacc
     7381 gataatcgca tgacccgcct cgtggtaagc cgtcgattct ttctgcgctt ccgtcatcac
     7441 catggagcga cgttccgcac ccatcatgat tttgtctttc gctttctcga actcaaccat
     7501 cgaaacaacg cgtttgttgc cacgagcagc gaacagtgcc gcttcgttca ccaggttcgc
     7561 caggtcagca ccggagaaac caggagtacc acgggcaatg attgccgcgt cgatatcggg
     7621 tgccaatggt acgcgacgca tgtgaacctt caggatctgc tcacgaccgc gaacatctgg
     7681 caagccaacc acaacctgac ggtcgaaacg gccaggacgc agcagggctg ggtcgagaac
     7741 gtccggacgg ttagtcgcag cgataacgat gataccttcg ttaccttcaa aaccatccat
     7801 ctcaaccagc atctggttca gagtctgttc acgttcatcg tgaccaccgc ccaagcctgc
     7861 gccgcgctgg cggcctacgg cgtcgatttc atcgataaag atgatgcacg gcgccgcttt
     7921 cttcgcctgt tcgaacatgt cacgaacacg ggatgcaccc acaccgacga acatttctac
     7981 gaagtcagaa ccggagatag taaagaacgg aactttcgct tcgcctgcaa ttgctttcgc
     8041 cagcaacgtt ttaccggtac ccggaggacc taccatcagg acgcctttcg ggatcttacc
     8101 gccgagtttc tggaagcggc tcggctcgcg caaatactca accagttcag caacttcttc
     8161 ttttgcttcg tcgcagcccg caacgtcagc aaaggtggtt ttgatctgat cttccgtcag
     8221 catgcgcgct ttgctcttac caaacgacat ggcacctttg ccaccgccgc cctgcatttg
     8281 acgcatgaag aagatccaga caccaatcag caacagcatc gggaaccagg agatgaagat
     8341 agaagccagc aggcttggtt cttcaggcgg ttcaccgaca accttgacgt tcttggtcaa
     8401 caggttatcc agtaatttcg gatcctgaac cggaatgtaa gtggtataac ggttactatc
     8461 tttcttggta acgttgattt cacgtccgtt gatacgcgct tcacgaacct ggtcgttatt
     8521 gacctcttgt aggaaggtag agtaatccac cttacggcca ttagactcgc tgggcccaaa
     8581 gctctggaat actgacatca gcacaacggc aatgaccagc cagagtatta ggtttttcgc
     8641 catgtcactc aagggattaa cctcttatta caactgtgtt aaaaacagcg tcaggatact
     8701 ctatatccag catctttcaa actttcgtct gaaatctccc ggttagggtt tacgcccggt
     8761 cgctacaata tacacttccc gcgaacgtgc acgagaagag tccggcttac gaactttgac
     8821 cttcgtaaac agggagcgaa tttcccttag atactcatcg aaaccttcgc cctggaacac
     8881 cttcactaca aaactgccac ctggcgctaa tacatcacga cacatttcta gcgccagttc
     8941 caccagatac atggcacggg ggatatccac cgccggtgtt ccgctcatgt ttggtgccat
     9001 atcggacatg acaacctgga ctttgctgtc gccaacgcgc tctagcagtg ctttcatcac
     9061 cagttcatca cgaaaatcgc cctgaagaaa gtccacacca acgataggat ccataggtaa
     9121 aagatcgcaa gcgatgatgc ggcctttgcc gccaatttgg gtgaccacat attgtgacca
     9181 accacccgga gctgcaccaa ggtcgacaac cgtcattccc ggtttaaaga gtttgtcact
     9241 ttgctgtatt tcatcaagtt taaaccaggc acgggaacgt aacccctttt tctgtgcctg
     9301 ttgaacatat ttatcgctaa agtgttcctg aagccagcgg ctggagctgg cagaacgctt
     9361 cttacctgtc atttaacttt cccatgggga taactcatcg taaccaattg cgtaaatttt
     9421 tacgcgccta tttggtgata tatgggagat ggcggtagaa tgacccgttt tcaatcccaa
     9481 cgtaagcaaa aaatacgatg aatctgagta ctaaacaaaa acagcacctg aaaggtctgg
     9541 cacatccgct caagccagtt gttctgcttg gcagtaatgg tttgaccgaa ggggtgctgg
     9601 ccgagattga acaagcgtta gagcaccatg aactcatcaa ggtgaaaatc gccaccgaag
     9661 atcgcgaaac taaaaccttg atcgtggaag ctatcgtgcg cgaaaccggc gcctgtaatg
     9721 tacaggtcat cggtaaaacg ctggtgcttt atcgcccaac taaagaacgt aaaatctcgc
     9781 tgccacgcta agattatcct aaagttacac acattcgctg tgtaaaacga gggtttttcc
     9841 gcaggcagga gagcaaaatg ccacgctctg ttcgttgata aaaggccgca tagcggcctt
     9901 tttcctttct ttacaataca tcaacatctt gagtattggg taattcttac aggtattcca
     9961 ccttaattac ttcaaattct acttcgccgc ccggcgtttt gatgaccaca acatcatctt
    10021 cttctttgcc gatcaggcca cgagcaatag gcgagtttac agaaatcagg ttttgtttaa
    10081 agtcagcttc gtcatcgcca acgatgcgat aagtctgttc ttcgtcagaa tcgagattca
    10141 gcaccgttac ggtagcacca aaaataacgc gcccattgtt gggcattttg gtgacatcaa
    10201 tcacctgcgc gttcgacagc ttggcttcga tgtctttgat acggccttcg cagaaaccct
    10261 gctgttcacg agctgcgtgg tattcggcgt tttctttcag gtcgccatgc tcacgcgctt
    10321 ccgcgatagc agcaatgatt tcaggacggc gcacagattt cagaaaatcc agctcttcgc
    10381 gtaatttttc agcgccgcgt aaggtcatcg gaatagcttg catttgttat acctcttgaa
    10441 tattcctgat ggggcaagtc ttcacccacc ccggctgttc ggcctgcccg gcataactcc
    10501 tcaccgggac cagaagcaaa aaaataccga cccgggtaca agtcccaggt cagctacaat
    10561 tcacattttg atagtcattt taccctggag ttcccgaagg gtcatcgttt actttatagg
    10621 gcgttgcgcc gtagtatgac ggctcgattc caggttgtta gcgcgagatt atgcgatttt
    10681 ccagatttat catcggattg accagctgta tagcgttcag tgttcaggcc gcaaatgttg
    10741 atgagtacat tactcaactc cccgcaggtg ccaaccttgc cctgatggtg caaaaagtcg
    10801 gcgcgtcggc ccccgctatt gattaccaca gtcagcagat ggcgctaccc gccagtacgc
    10861 agaaagtgat tactgcgctg gcggcgttga ttcaactcgg ccccgatttt cgttttacca
    10921 cgacgcttga aaccaaaggc aatgtggaaa acggcatact taagggtgac ttagtagcgc
    10981 gatttggtgc cgatccgacg ttaaaacgtc aggatattcg caatatggtc acgactttga
    11041 aaaaatctgg cgtcaaccaa atcgatggca atgtgttgat agatacctcc attttcgcca
    11101 gccacgataa agcccccggc tggccatgga atgacatgac acaatgcttt agcgctccgc
    11161 ctgccgccgc catagttgac cgcaactgtt tctccgtctc gctctacagt gccccaaaac
    11221 ctggtgatat ggcttttata cgcgtggcat cttattaccc cgttacgatg ttcagtcagg
    11281 tacgcaccct cccccgtggt tctgccgaag cgcaatactg cgaactggat gtcgtacctg
    11341 gggacctgaa ccgctttacg ctaacgggat gcctgccaca acgttctgag ccgctcccgt
    11401 tggcttttgc cgtgcaggat ggtgccagct atgccggggc aattctgaaa gatgagttaa
    11461 aacaggcggg tatcacctgg agcggaacgc tgctgcgcca gactcaggtt aacgaacctg
    11521 gaacggtagt tgccagtaaa cagtcggccc cgctgcacga tctgcttaag attatgctga
    11581 aaaagtcgga caacatgatt gccgatacgg ttttccgcat gatcggccat gcgcgcttca
    11641 atgtgcctgg aacatggcgg gccggatcgg acgccgtgcg tcagatcctg cgccagcaag
    11701 ccggtgtcga tatcggaaac accattattg ccgatggttc agggctttcg cggcataacc
    11761 tgattgcccc cgccaccatg atgcaggtgc tgcaatatat tgcccaacac gacaatgaac
    11821 ttaactttat ctccatgctg ccgctggcgg gctatgacgg ctctttgcag taccgtgcag
    11881 gtctgcatca ggcgggcgtg gatggaaaag tctcagcgaa aaccggttcg ttgcaggggg
    11941 tatataacct ggcgggattc attaccacag cgagcgggca acgaatggcg tttgtgcaat
    12001 atctttctgg ctatgcagta gaaccggcgg atcagcgtaa tcgccgtatt ccgttagtgc
    12061 gttttgaaag ccgtttgtat aaagatattt atcagaataa ttagcctaaa gagaaacccc
    12121 ggcacatggc cggggttttg ctgattaacg cttgtaaatg aactcaacgc cttcttcgtc
    12181 gtcttcgtcc cagtcgtcat cccagtcctc atcatcctct tcagcaatct cttcaagctg
    12241 ctgacggtga taatcatccc acatgaattc gactttctct ggctgtttcg cttcttcagc
    12301 ctgcacgacc gggttttcaa tgataaaggt catcacatcc cagcagagat ctttcacgcc
    12361 cagtccgctc gccgcagaga tcagataata tttatcttcc cagcccagcg cttcagcgat
    12421 cgctttcgct ttctcttcgg cttctgcctt atccagcaga tcgatcttgt tgaagactaa
    12481 ccaacgcggt ttcgccgcca gatcctggct gtatttttcc agctccctga taataatacg
    12541 cgcgttttca accggatcgg tgccgtcaat cggatcgata tcgatgaggt gcaacaggac
    12601 gcggcaacgt tccaggtgct tcaggaagcg aatgcccaga cctgcgcctt ctgcagcgcc
    12661 ttcaatcagt cctggaatat cggcaacaac gaagctcttt tcgttgtcca tacgcaccac
    12721 gcccaggctt ggtaccagag tggtaaacgg ataatccgcc actttcggtt tagccgccga
    12781 taccgcacga ataaaggtcg atttacccgc gtttggcatc cccaacatac cgacgtcagc
    12841 cagcagcatc agctccagca gcagctcacg cttatcgccc ggcgtaccgt tagttttctg
    12901 ccgtggtgta cggttaactg acgacttgaa acgggtattg cccagaccgt gccagccgcc
    12961 cttagcaacc agcagacgct gaccgtgttt ggtcatatcg cccatggttt caccggtacc
    13021 ctggtcgatt acacgcgttc ctactggtac tttaatcgtg acgtctttac cgcgcttacc
    13081 ggtacagtct cggcttgcac cgttctgacc gcgctctgca cggaaagatt tttcaaaacg
    13141 ataatcgata agcgtgttca ggttctcgtc ggcttccatc catacgtcac caccatcacc
    13201 gccgtcgccg ccatccgggc cgcctttcgg aatatacttt tcgcggcgga aactcacgca
    13261 accattaccg ccatcacctg caacgaccag aatcgatgct tcatcaacaa acttcatttt
    13321 attctccgta aatcattcgc ctgcgcgggg ttgcgatacc accgttgtat gcttacgtaa
    13381 tccgccccaa atacgatgac caatggcgga atacatcgcg cccgcaacca cgacaaacgc
    13441 accgagataa cctaaaaggt ttaacatcgg tctggcgaag aaatcgggcc aggccagtga
    13501 taaaagatct gaaaaaaaca gcgtaaacag tggggtgagc gtgatgatcg cgctcacctg
    13561 cgctgcctgc cagcgagcca tcgcttccgc cagggcgcca tatcctacca atgtattcag
    13621 tccgcaaaaa attaaacatg cgagctgcca gtggctaagc tgcgctatca ctccaggatc
    13681 tgccagaggg aagagcgcaa ttgtacataa agtgtacagt aaaaacagga tctgcggtga
    13741 ggccagccga cgcaataaaa ccttttgcgc cacgccataa ctcacccaaa ccgtcgccgc
    13801 accgacccca aagataactc cccaggtgta atcggtgagc tttgtaaata tctcgaccag
    13861 actggtgtta aaaaacatca ccaggccgct caggagcatc aatgccccta caacctgagt
    13921 actgcgcatt ttctctttca ggataaatac gctggcaacc atcatgccaa ctggtgagag
    13981 ttgcccaatc acctgcgaag cggtcggact caggtattgc aaggatgagc tgaacaggat
    14041 gaagttccca aacagcccgg cagtcgccac tgctaacaaa atcaaccagc gtggcttacg
    14101 aaacacgcgt aatggcggca accgcttctt caccgcaaga atggcaccaa ggccaatact
    14161 cgccatcaag aaacggtaaa acacgattgt cggaggttcc atcacctcca gcacctgctt
    14221 cattgcgatt ggcaacgccc cccagcaaat tgctgtggtg agcgccaaaa gaatgccaat
    14281 gcctgcctgc tgcttcatac cgtattccct gcaaagcgca tatgcggatt aacgtcgcac
    14341 catgcgcatt tttaccggtt atcgaatgta aaaagccccg caacgtgttg cggggctttc
    14401 atccgttacc gggacgcgaa aaacttattc agcttcgatg ctgataaatt tacggttttt
    14461 cgggccttta acttcgaatt tcactttacc gtctgcttta gcaaacagag tgtggtcacg
    14521 accgcaacct acgttagcgc cagcgtggaa tttggtacca cgttgacgaa cgatgatgct
    14581 acccgccaga acggattcgc caccgaaacg cttaacgccc aggcgtttag cttctgaatc
    14641 gcgaccgtta cgtgtggagc cgccagcctt tttatgtgcc atttgaaatc tctcctcagg
    14701 tcttaggcgc tgatgccagt aattttcaca tcagtgaacc actgacgatg gccctgctgc
    14761 ttacgatagt gtttacgacg acgaaactta acgattttaa ctttctcgcc acgaccgtga
    14821 gcaacaactt cagctttgat tacgccgcca tcaacgaaag gaacgccgat tttgacttct
    14881 tcaccgtttg cgatcatcag cacttcagcg aactcaacag tttcgccagt tgcgatgtcc
    14941 agcttttcca ggcgaacggt ctgaccttcg cttactcggt gttgtttacc accactttgg
    15001 aaaaccgcgt acatataaaa ctccgcttcc gcgcacacct tttcaatgat tcagagtgcg
    15061 ctataaatat tcacaatagg gcgcgaatat tacgcaaaac gcacgccttt gacaagtgct
    15121 acagtcaata cacgaagaaa aaaaacacaa cttgtacggt aacgtttatc tgtgccattt
    15181 tttcagtaca atcaccctat attcctaacc ataaacccta agttgccttt gttcacagta
    15241 aggtaatcgg ggcgaaaagc ccggcttttg cgatgaattt agaaaaaatc aatgagttaa
    15301 ccgcgcaaga tatggcgggt gttaatgcgg caatccttga gcagcttaat tccgacgtcc
    15361 aactgatcaa tcagttaggc tattacatcg tcagcggcgg cggtaaacgt attcgtccga
    15421 tgattgctgt actggctgca cgagctgttg gctatgaggg aaatgcgcat gtcaccattg
    15481 ctgccctgat cgagtttatc cacacggcaa ctctgctaca cgacgacgtt gtggatgaat
    15541 cagatatgcg caggggtaaa gctaccgcca acgccgcatt tggcaatgcc gccagcgtgc
    15601 tggtaggcga ttttatttat acccgcgcgt tccagatgat gaccagcctc ggttcactca
    15661 aagtgctgga agtcatgtca gaagccgtaa acgtcatcgc agaaggtgaa gttctgcaac
    15721 tgatgaacgt taacgatccg gacatcactg aagaaaacta catgcgcgtt atctacagca
    15781 agactgcgcg tctgtttgag gctgccgcgc agtgttccgg gattctggct ggctgtacgc
    15841 cggaggagga gaaaggccta caagattatg ggcgctatct cggcactgct ttccagttga
    15901 tcgacgattt actcgattac aatgccgatg gcgaacagtt aggtaaaaac gtcggcgacg
    15961 atctgaacga aggtaaaccg acgctgccgc tgctgcatgc gatgcatcat ggcacaccag
    16021 aacaggcaca gatgattcgt accgccatcg aacagggtaa cggtcgccat cttctggaac
    16081 cggttctgga agcaatgaac gcttgtggat ctcttgaatg gacgcgtcag cgtgccgagg
    16141 aagaagcaga caaagccatc gcagcgttac aggtgctccc ggacaccccg tggagagaag
    16201 cactcgtcgg cctcgcgcac atcgctgttc aacgcgatcg ttaatcccct cccctcatcc
    16261 cgcgcagtgc gcgggatgat tccagtaaat tccataaaac acttattcag ctctataact
    16321 cctgaaaaac ggggctctgt atctttatat attctgaata ttcacactct ttacaggaac
    16381 tttttagagc aataggccat caggagtata gtgatgctcg acagaaacag aagaagtgtt
    16441 ctgaatgaaa gcgactaact taaggagtga ggaaagatgg aaagtaattt cattgactgg
    16501 catcccgctg acatcattgc aggtttgcgc aagaagggaa cgtcaatggc ggcggagtct
    16561 cgcagaaatg gtttgagttc ctcaacgctg gcgaacgcat tatcacgccc atggccgaaa
    16621 ggagagatga ttattgcgaa agccctggga actgacccct gggttatctg gccatcacgc
    16681 taccatgatc cgcagaccca tgagtttatc gacagaacgc agttgatgcg tagctacact
    16741 aaaccgaaaa aatgagtggt ctggcggtag ccccgcgaac ggggctgcca gctctcagac
    16801 aattactcgc ctttcacacg ctcaatattt gcacctaaag cgcgtagttt gtcttcaatg
    16861 cgttcgtagc cacgatcgat gtgataaata cgatcaacca ccgtcgtccc ttccgcaata
    16921 cagccagcca gcaccaggct tgctgatgca cgcagatcgg ttgccataac ctgtgcgcca
    16981 gaaagttttt caacaccgtg acaaataacg gtattgcttt cgatttcggc gtgcgcgccc
    17041 atacggctca gctctggcac atgcataaag cggttttcaa agaccgtttc ggtgatgaat
    17101 ccggtccctt ctgccaccag gttcaacagc gtgaactggg cctgcatatc ggtcgggaat
    17161 gccggatgcg gcgcggtacg tacgttaaca gccttcggac gtttgccatg catatccagg
    17221 ctaatccagt cttcgccgac ttcgatgtcc gctccagcgt cacgcagttt cgccagcacg
    17281 gcgtccagag tatctggctg cgcgttacgg cagataattt tgccgcgaga gatcgccgcc
    17341 gccaccagga aagtaccggt ttcgatacga tccggcagca cgcgatagac accgccgcct
    17401 aaacgttcca caccttcgat gacgatacga tcggtgccct gaccgctaat tttcgcaccc
    17461 agcgtaatca ggaagttcgc ggtatcgacg atttccggtt cacgcgctgc gttttcaata
    17521 atcgtggtgc cttccgcaag ggttgcagca cacatgatgg tcaccgttgc gccaacgctg
    17581 actttatcca tcacgatatg tgcgcctttc aaacgaccat cgacggaagc tttaacgtaa
    17641 ccttcttcca gtttgatggt cgcgcctaat tgttcgaggc cagaaatgtg tagatcaacc
    17701 ggacgtgcgc cgatcgtaca accgccaggc agtgaaactt gcccctgacc aaagcgcgct
    17761 accagcggcc ccagcgccca gatagaagca cgcatggttt taaccagatc gtaaggtgcg
    17821 cagaatacat taacgtcgcg ggcatcaata tgcacagaac cattacgttc tactttcgca
    17881 cccagctggc ttagcagctt cattgatgta tcgacgtctt tcagtttcgg gacgttctgg
    17941 atctctaccg gttcttccgc cagtagtgcg gcaaaaagga taggcagagc agcattttta
    18001 gcgccggaaa ttgtgacttc gccctggagc ttcgttggcc cctgaacacg aaatttatcc
    18061 atttagtttg ttctcagtta acaattcata tccgctaccg gcgaatcgcc catagctcaa
    18121 aagccgttca gtttgcgatc gcgcgcccac tccgcagggg tatacgcttt gatcgacaca
    18181 gcatgaatgc ggttatccgc aatatattcc atcagcgggc catagaccgt ctgctgtttt
    18241 ttaacccgac tcatgccgtc aaacaactca cccacggcaa taacctgaaa gtggctgcca
    18301 tcgccggaaa cgtggacttc ctggagggag agagcgttca tcaacacgct ctgaatttca
    18361 ttattttcca tgggatcttc aatcatcagt taataaatca gcgaaacatc ttagagcaaa
    18421 gttgcgctgg cataaataag caaaaagcct cgctgataaa tcagacaagg ctcgacttgc
    18481 aggcaggttt gccggacagg cggttaacgc catatccggc ctgaaaaaat ttaacgaggc
    18541 agaacatcag caggcaaatt atacaatttc gccagggtat acactttatc gtttaccccc
    18601 tgaagcgtca cgttgttgcc ctgctttttc gccagatcga taagatggag cagcagtgcc
    18661 agtccacccg tatccacgcg ggacacacgg ctaagatcga tgcaggtaat ccccttcacc
    18721 gcttcctcgc gcatttccca aagcggtagc agaacgtcct gatccagctc tccggatagc
    18781 gccagcgtgt cacccgtctg catccagctc agtgactcgc tcattatttt ttctcttcca
    18841 gagtgatttt ctgttgagaa atcgatttca gttgtgcagt caggccgtcg atacctttgg
    18901 tacgcagcag cgttccccac tcgttttgtt tggtggtgat catactgacg ccttcagcaa
    18961 tcatgtcgta agcctgccag ttgcccgtct gggagttttt acgccactgg aagtccagac
    19021 gcaccggcgg gcggccattc gggtcaataa tggtaacgcg aatcggcaca atggttttat
    19081 cacccagcgg ctgttctggc gcaatctgat aggtttgacc gtgatacatc gccagcgcct
    19141 gaccgtaagc ctgcttcagg tactcacgga aagcggcaaa gtaagcatca cgttgagctg
    19201 gggtcgcact cttgtaatac tggcccagca ccagcgcacc ggcgtatttc acctgtacgt
    19261 atggcaacag ttcctgatca acaatggtac gcagataatc cgggttggcc cgaatttgcg
    19321 gttgctcatt cttcaggcga tcgaacgttt tctgcgccgc ctcgtccatc agcttatacg
    19381 gattggtctg gtctgccgcg gttgccgcac tcagcggtgc aatcaccagc aaagcgacca
    19441 tcattaaacg tttaaacatg cgtcggttct cctgaaatta tttcgttgta cccacaggtt
    19501 cagtggtttc attattacct ggcgcagcag ctggcgcatc gccactattc ttattgtcat
    19561 cgcctttact accgtaaagg aactgaccaa tgagatcttc cagcaccatc gcagacttgg
    19621 tgtcctgaat tgtatcgcca tccttcagga tagcagtccc cagttccggg tcttcaaaac
    19681 cgacgtttaa tgccagatat tgttccccca gcaggccgga agtacgaatg ctcagcgaac
    19741 tggtatcagg aatatggtta taacgttgtt caatttccag cgttacgcgc ggcagatagg
    19801 ttttcgggtc cagcgtaata tccgccaccc ggcccacaac aacgccacca atgctgaccg
    19861 gagagcgcgc tttcaggccg ccaatgttat cgaacgtcgc ataaagcgta taggtcggtt
    19921 cagtacgtat agacgtcacg ttcgctgcct tcaggcaaac aaacagcgcc gcaagcagtg
    19981 ctgctaataa aaagataccc acccaaattt catttttttt cgtttgcatg aactcaattc
    20041 ccaaacatca atgcggtcag cacaaaatcc agccccagaa cagccagaga cgagtggaca
    20101 acggtgcgag tggttgcccg gctaatcccg gcagacgtcg ggatggcgtc gtagccgtta
    20161 aacaacgaaa tccacgtcac cgtgatggcg aacactacgc tcttaatcag acagttgacg
    20221 agatccatac gccagtcgac ggcattttgc attgccgacc agaagaaccc actatcaatg
    20281 cctttccaac tgacgccgac cagcgatccg ccccagatcc ccacggcgac gaagataacc
    20341 gtcaacagtg gtaatgaaat aactccagcc cagaaacggg gagaaataac ccgacgcagc
    20401 ggatccaccg ccatcatctc catactggag agttgctctg tagcgcgcat caggccgatt
    20461 tctgcggtta gcgccgaacc agcacgcccg gcaaacaaca acgcggcaac caccggcccc
    20521 agttcacgca gtagcgataa cgccaccagc atacccagac tggtttccgc actataagtg
    20581 gtcagaacca gataaccttg cagccccaac accattccga tgaacacgcc agaaaccaca
    20641 ataatcagca tcgacaggac gccgacatta tagagctggc gcaccagcag cggtgcatgt
    20701 ttgcgaaatt ccggtttgcc gaccagcgca ttgaataaca ttaacccggc ccgcccgaac
    20761 gttctcaggg ttttaatccc tttatgtccg agcgacgcca gcgcatttaa cagcatgagt
    20821 ggcttaactc cctggtaaaa gatcagcgtg ataatcgccg gcaggatagc ggaacggaac
    20881 aggcccgtca gctatcccgt ccagaaactg acgtacgcgc ggatcaggat tcgcctgcaa
    20941 cgcctgggca ctgccatgag cgacaatttt tttgtccgcc aggatccagg cgtgatccgc
    21001 aatacttaac acttccggca catcgtgaga aaccaccaca caagtcacgc ccagcgcgct
    21061 gttcagctca gaaatcagct tcaccagtac gcccatggta atgggatctt gcccaacaaa
    21121 gggctcatca aacatgatga gatccggctc cagcgcaatc gcacgtgcca gcgctgcacg
    21181 ccgcgccatc ccaccggaaa gttcagaagg cataagttta gccgctccac gcagccccac
    21241 ggcctccagc ttcatcatca ccgtactatg caataatggc gcgggaagtt gggtatgttc
    21301 ccgcagtgga taggcgacgt tgtcaaatac gttcatatca gtgaacaacg ccccggactg
    21361 aaataacatg ctcatccgtt tgcgcactgt atacaggcgc gaacgagaca tcgccggaat
    21421 attctcacca tcgaaaagga tctcaccatg atctggtgcg atttgcccgc caatcagacg
    21481 gagtagcgtc gttttaccga tgcccgatgg ccccatgatc gccgtgatct tccctctcgg
    21541 cacggtcagg gaaatattat cgaagatgca gcgattgcca cgcgtaaaac tgacatcgcg
    21601 catatcgact aaattcgcca cagactgctc cataattcac ccttcgtctt gcgttgattt
    21661 tctaagcatg gcgctcaatt taaccttgaa cccaacatat ttacagaata ttacctgccg
    21721 tggttagcga aagctggcat ttgttttact ttttagccgc ataaagtcaa aattaagaat
    21781 ctgttacggc tttctgaaaa tcttcagcgg accggcgagt atacctgaag aaaggacgtt
    21841 agatgctttt agctacggca ctgttaattg ttggtttact tttggtcgtt tacagtgccg
    21901 accgcctggt ttttgccgcg tctattcttt gccgaacctt tggcatcccg ccgctgatca
    21961 tcggcatgac ggtggtcagt attggtacat cgttaccaga aatcatcgtc tcgcttgccg
    22021 cgtctttgca cgaacaacgc gatttagccg ttggtacagc cctcggctca aacattatta
    22081 atatattgct gatcctcggt ctggctgcgc tggttcgtcc ttttaccgtc cattctgatg
    22141 ttctacgccg tgaattaccc ttaatgttat tggtcagcgt ggtggccggt tccgtactct
    22201 atgacggaca acttagtcgc agcgatggta tctttctcct ctttctggct gtgctatggc
    22261 tgctgttcat tgttaaactt gcacgtcagg ctgaacgtca ggggactgac agcctgacca
    22321 gagagcagct tgcagagctg ccgcgtgaag gtggattgcc cgtcgcattt ttatggctcg
    22381 gcatcgcact gatcattatg ccagtggcca cgcggatggt agttgataac gccacggtgc
    22441 tggcgaatta ctttgccatt agcgagttga cgatgggtct gacggcaatt gctatcggaa
    22501 ccagcctgcc ggaactggca accgcaatag cgggggttcg caaaggtgaa aacgacattg
    22561 cggtcggaaa tattattggc gcaaacatct ttaatattgt catcgtgttg ggtttacccg
    22621 cgctgataac gccaggagag attgatccac tggcgtacag tcgtgactac agcgtgatgt
    22681 tgctggtgag cattattttt gcgttgctat gctggcggcg ctccccgcaa ccgggccgtg
    22741 gtgtaggggt attattaact ggcggattta tcgtatggct ggcgatgttg tactggttat
    22801 cgccaatact cgttgaataa ctggaaacgc attatgtcgc acgtagagtt acaaccgggt
    22861 tttgactttc agcaagcagg taaagaagtc ctggcgattg aacgtgaatg cctggcggag
    22921 cttgatcaat acatcaatca gaatttcacg cttgcctgtg aaaagatgtt ctggtgtaaa
    22981 gggaaagttg tcgtcatggg gatgggcaaa tcggggcaca tcgggcgcaa aatggcagca
    23041 acatttgcca gcaccggtac accttcattt ttcgtccatc ctggtgaagc cgcgcatggt
    23101 gatttaggca tggtcacccc gcaggatgtg gtgattgcta tctctaactc aggtgaatcc
    23161 agcgaaatca cggccttaat tccagtgctt aagcgtcttc acgtaccgtt aatctgcatc
    23221 accggtcgcc cggagagcag catggcgcgc gccgcagatg tgcatctgtg tgttaaagta
    23281 gcgaaagaag cctgtccgtt agggctggca ccgaccagca gcacaaccgc cacgctggtt
    23341 atgggcgatg ccctcgctgt cgcgctgtta aaagcacgcg gctttactgc tgaagatttt
    23401 gcgctctcac acccaggcgg cgcactgggt cgtaaacttc tgctgcgcgt aaacgatatt
    23461 atgcatacgg gcgatgagat cccgcatgtt aagaaaacgg ccagtctgcg tgacgcattg
    23521 ctggaagtta cccgcaaaaa tcttggtatg actgtcattt gcgatgacaa tatgatgatt
    23581 gaaggcatct ttaccgacgg tgatttacgc cgtgtcttcg atatgggcgt ggatgttcgt
    23641 cagttaagta ttgccgatgt gatgacgccg gggggaatac gcgtgcgccc aggcattctg
    23701 gccgttgagg cactgaactt aatgcagtcc cgccatatca cctccgtgat ggttgccgat
    23761 ggcgaccatt tactcggtgt gttacatatg catgatttac tgcgtgcagg cgtagtgtaa
    23821 agattcaagg ataaacaaca atgagcaaag caggtgcgtc gcttgcgacc tgttacggcc
    23881 ctgtcagcgc cgacgttatg gcaaaagcag agaacattcg tctgctgatc ctcgacgttg
    23941 atggcgtact gtcagatggc ctgatttata tgggcaataa cggcgaagag ctgaaagcgt
    24001 tcaatgttcg tgacggttat ggcattcgtt gtgcgctcac ctctgatatt gaagtcgcta
    24061 tcattaccgg gcgaaaggct aaactggtag aagatcgttg tgccacattg gggatcactc
    24121 acttgtatca ggggcagtca aacaaactga tcgcctttag cgatctgctg gaaaaactgg
    24181 cgattgcccc ggaaaatgtg gcttatgtcg gcgatgatct catcgactgg ccggtaatgg
    24241 aaaaagtggg tttaagcgtc gccgtggccg atgcgcatcc actgttgatc ccgcgcgccg
    24301 attacgtaac gcgcattgct ggcggtcgtg gcgcagtgcg agaagtttgc gacttattac
    24361 tcctggcgca gggcaaactg gatgaagcca aagggcaatc gatatgagta aagccagacg
    24421 ttgggttatc attgtgctat cactggcggt tctggtgatg atcggcatta atatggccga
    24481 aaaagacgat accgcccagg tggtcgtcaa caacaatgat cccacctata aaagcgagca
    24541 tacggacacg ctcgtctata acccagaagg ggcactaagc tatcgattga ttgctcaaca
    24601 cgttgaatat tattccgatc aggccgtttc gtggtttacg cagccggtac ttaccacgtt
    24661 tgataaggat aaaatcccga catggtccgt aaaagcagat aaagccaagc tgaccaatga
    24721 ccggatgctc tatttatatg gacacgttga agtcaatgca ctcgtgccgg actctcaact
    24781 tcgcagaatc acgacggata acgcgcagat caatctggtg acgcaggatg ttacctctga
    24841 agacctcgtc acgttatacg gaacaacatt taactccagc ggtctgaaaa tgcgcggcaa
    24901 cttacgcagc aagaacgccg agctgattga aaaggttaga acatcctatg aaattcaaaa
    24961 caaacaaact cagccttaat cttgtgcttg ccagctcact tctggccgcc agcattccgg
    25021 catttgccgt aaccggagat actgatcagc cgatccacat tgaatcggac cagcaatctc
    25081 ttgatatgca aggcaacgtg gttaccttta ccggcaatgt catcgtcacc cagggcacca
    25141 tcaaaattaa tgccgacaaa gtggtcgtta cccgtccggg cggcgaacaa ggtaaagaag
    25201 tgattgacgg ctacggtaaa ccggcaacgt tctaccagat gcaggacaac ggtaaacccg
    25261 ttgaaggtca cgcttcccag atgcactacg aactggcaaa agattttgtc gttctgacgg
    25321 gtaatgctta tctgcagcag gtcgatagca acattaaggg cgataagatc acttacctgg
    25381 tgaaagagca gaaaatgcag gctttcagcg acaaaggcaa gcgcgtaacc accgttctgg
    25441 tgccgtcgca gctgcaggac aaaaacaata aaggccagac cccggcacag aagaagggta
    25501 attaattcgt tatggcaaca ttaactgcaa agaaccttgc aaaagcctat aaaggccgtc
    25561 gcgtggtaga agacgtcagc ctgaccgtca actccgggga aattgtcggt ctgctggggc
    25621 caaacggtgc cggtaagacc accactttct acatggttgt tggcattgtg ccgcgcgatg
    25681 cgggcaacat cattattgat gatgacgata tcagtctgct gcctctgcat gcacgcgcgc
    25741 gccgcggtat cggctatctg ccgcaggaag cctccatttt ccgtcgcctc agcgtttacg
    25801 ataacctgat ggcggtactg caaattcgtg acgacttgtc tgctgaacaa cgtgaagacc
    25861 gcgcgaacga gctgatggaa gagtttcaca ttgagcacct gcgtgacagc atggggcagt
    25921 cactctccgg gggtgaacgt cgccgtgtag aaattgcccg cgcactggct gcgaatccga
    25981 aatttattct gctcgacgaa ccgtttgccg gggttgaccc gatctcggtt atcgacatta
    26041 aacgcatcat tgagcacctg cgcgacagcg gcctgggcgt actgatcact gaccacaacg
    26101 tgcgtgaaac actggcggtt tgtgaacgcg cttatatcgt cagtcagggg catttgatcg
    26161 cccacggcac gcctacagaa atcttacaag acgaacacgt taagcgtgta taccttgggg
    26221 aagacttcag actctgatag ggtagaagtt tgcgacgttt tagcaggaga gtacgattct
    26281 gaacatgaag caaggtttgc aactcaggct tagccaacaa ctggcgatga cgccacagct
    26341 ccaacaggca attcgtctgt tgcagttgtc gacgctggaa cttcagcagg agctacagca
    26401 ggcgctggag agcaatccgc tgcttgagca aatcgacact catgaagaaa tcgacacccg
    26461 cgaaacgcaa gacagtgaaa cactggacac cgccgacgcg ctcgaacaaa aagagatgcc
    26521 ggaagagctg ccgctcgatg ccagttggga caccatttac accgctggta caccatccgg
    26581 caccagcggt gactacattg acgacgagct gccggtctat cagggcgaaa cgacacagac
    26641 cttacaggat tacctgatgt ggcaggtcga gttgacaccg ttttccgaca ctgaccgcgc
    26701 tattgctacc tctatcgtcg atgccgttga tgacaccggt tatctgactg tcccgctgga
    26761 agatattctc gaaagtatgg gcgatgaaga gattgacatc gacgaagttg aagccgtcct
    26821 taagcggatc caaaggtttg atccggtcgg tgtggcggca aaagatctgc gtgactgcct
    26881 gctgatccaa ctctcccaat tcgataagac cacgccatgg ctggaagagg ccagactgat
    26941 cattagcgat catctcgatc tattagccaa tcacgacttc cgcactttaa tgcgcgtcac
    27001 gcgtctgaaa gaagatgtgc tgaaagaagc cgtcaatctg atccagtcgc tcgatccgcg
    27061 ccccgggcag tcgatccaga ctggcgaacc tgagtatgtc attccagatg tgctggtgcg
    27121 taagcataac ggtcactgga cggtagaact caacagtgac agcattccgc gtctgcaaat
    27181 caaccagcac tacgcctcga tgtgcaataa cgcgcgcaac gatggtgata gccagtttat
    27241 ccgcagcaat ctgcaggatg ccaaatggtt gatcaagagt ctggaaagcc gtaacgatac
    27301 gctactgcgc gtgagtcgct gtatcgttga acagcagcaa gccttctttg agcaaggcga
    27361 agaatatatg aaaccgatgg tactggctga tatcgcccag gccgtcgaga tgcatgaatc
    27421 gacgatatct cgcgtgacca cgcaaaaata cctgcatagt ccacgaggca tttttgaact
    27481 gaagtatttc ttttccagtc acgtcaatac tgaaggcggg ggtgaagcct cttccacagc
    27541 gattcgcgca ctggtgaaga aattaatcgc ggcggaaaac ccagcgaaac cgttgagcga
    27601 cagcaagtta acctctttgc tgtcggaaca aggtatcatg gtggcacgcc gcactgttgc
    27661 gaagtaccga gagtctttat ccattccgcc gtcaaaccag cgtaaacagc tcgtttgacc
    27721 caaccgataa ggaagacact atgcagctca acattaccgg aaataacgtc gagatcaccg
    27781 aggcactgcg cgaatttgtt acagccaaat ttgccaaact tgagcaatat tttgaccgaa
    27841 tcaaccaggt ctatgttgtt ctgaaagtgg agaaagtcac ccacacctca gatgcaacac
    27901 tgcatgtaaa cggtggtgaa attcatgcca gcgcggaagg tcaggacatg tacgctgcca
    27961 ttgatggctt aattgataag ctggcacgcc agttgaccaa acataaagat aaactgaaac
    28021 aacactaatt gtccgggcaa ttagcatgtg catggcggcc tgttgtgcgg cacaacgggc
    28081 catttgtacg gttaatgctc cgagcctgtt ccactgtttg agtgggcagg ttcttaggtg
    28141 aaattatgac aaataatgat acaactctac agcttagcag tgttcttaac agggaatgta
    28201 cgcgaagccg cgtccactgt cagagcaaaa aacgcgccct ggaaatcatc agcgagctgg
    28261 cagcgaaaca acttagcctg gcgccccagg tggtttttga agctatcctg acgcgggaaa
    28321 aaatgggcag taccggtatc ggcaatggta ttgccattcc gcatggcaaa ctggaagaag
    28381 atactctgcg cgccgttggc gttttcgttc agctcgaaac gcctatagct ttcgatgcca
    28441 tcgacaacca accggtggac ttactttttg ccctgctggt gccggcagac caaactaaaa
    28501 cgcacctgca tactctgtcg ctggtagcga aacgtctggc ggacaaaacc atctgccgcc
    28561 gtttacgcgc agcccagagc gatgaagagc tgtatcaaat cattacggat accgaaggta
    28621 ctccggatga agcgtagtta ttcggtaatg tctcttttag acgttgtgag gagaaacagt
    28681 acatggtact gatgatcgtc agcggacgtt caggttcagg taaatctgtc gccctgcgtg
    28741 cgctggaaga tatgggtttt tactgcgtgg ataaccttcc cgtagtgttg ttacccgatc
    28801 tggctcgaac tctggccgat cgagagattt ctgccgccgt cagcattgat gttcgtaata
    28861 tgccggagtc accagaaata ttcgaacagg cgatgagtaa cctgcctgac gctttctcac
    28921 cgcaactact gttcctggat gccgaccgta ataccttaat tcgtcgttac agtgacacgc
    28981 gccgactgca tccgctttcc agcaaaaacc tgtcgctgga aagtgctatc gacaaagaaa
    29041 gcgatttgct ggagcctctg cgttcgcgag cggatctgat tgtcgatacc tcagaaatgt
    29101 ccgttcacga gctggcagaa atgctgcgta cccgtctgct gggtaaacgt gaacgtgaac
    29161 tgaccatggt ctttgagtct ttcggcttca aacacggtat ccctatcgat gcagattacg
    29221 tctttgacgt gcgcttcttg ccgaacccgc actgggatcc gaaactgcgt ccaatgacag
    29281 gtcttgataa acctgtcgcc gcgttcctcg accgccacac agaagtacac aattttatct
    29341 accagacgcg aagctatctt gagctatggt tacctatgct ggaaaccaac aaccgtagct
    29401 acctgacggt cgccattggt tgtaccggcg ggaagcaccg ttcggtgtat attgcagagc
    29461 aactggcaga ctacttccgc tcgcgcggta aaaacgtcca gtcacgccat cggacgctgg
    29521 aaaaacgtaa accatgaccg tcaagcaaac tgttgaaatc acaaacaagc tgggcatgca
    29581 tgcccggcct gcaatgaagc tgtttgaatt aatgcagggt tttgacgctg aagtgctgtt
    29641 acgtaatgac gaaggcaccg aggctgaagc caacagcgtt attgcgctgc tgatgttgga
    29701 ttctgccaaa ggacggcaga ttgaagttga agcgaccggt ccacaggaag aggaagcact
    29761 ggccgccgtt atcgccctct ttaactctgg ttttgatgaa gattaatctt catcactttg
    29821 acattcaaat cccttcaagc tcccccggca ataagaaaag cgcgtactga aatcgttatc
    29881 aatcaacgtc gattgtacat ttcgtgcgct tttttacttt cagaagaacc ttaagaaaac
    29941 cttaagaagc attgtttaga tttcgtttaa gttaatcgac catactggag atcgtcagaa
    30001 aatatttcca ggagatggca tgattcgctt atctgaacaa agtcccctgg gcaccggaag
    30061 acatcggaaa tgttatgcgc atccggaaga tgcccaacgc tgtatcaaga ttgtctacca
    30121 tcgtggcgat ggtggcgata aagagatccg ccgtgagtta aagtactacg cgcatcttgg
    30181 tcgccggtta aaagactgga gtggaatacc gcgctatcac ggtaccgttg agacggattg
    30241 cggaaccggt tatgtctacg atgtgattgc cgatttcgac ggcaagcctt ccattacgct
    30301 gaccgaattt gcagaacaat gccgttacga agaagacatt gcacaactgc gccagttact
    30361 gaaacagctg aagcgttatt tgcaggataa ccgtatcgtg acgatgtcgc tgaagccgca
    30421 gaatatcctc tgtcaccgca tcagtgagtc agaggttacc cccgtcgtct gcgacaacat
    30481 tggtgaaagc acattgattc cactggcgac ctggtcgaag tggtgttgtc tgcgtaagca
    30541 agagcggtta tggaaacgat ttatcgccca accggcgctg gctattgccc tgcagaaaga
    30601 tctgcaacct cgtgagagca aaacgctggc cctcacttcg cgcgaagctt aatcaagctg
    30661 gtgttgttgc ataaacggct caccgcctaa ctgatacatc tgccgtaaaa tccacgcctg
    30721 acggctacgc acgtagcccg atggcgcgga gactttgaaa cgaagtggat taggtaatac
    30781 agctgccagc aatgcagctt ccgaccgggt aagtttgctc gcgggtttgt ggaaataacg
    30841 ttgtgccgca gcttcgacgc caaacacacc gtcgccaaat tcggcgatat tcaggtaaac
    30901 cgtcaggata cgctttttgc tccagactgt ttctatcccc agcgttaatc cggcttccag
    30961 cccttttcga acccagctac gcccatccca taaaaagaga tttttggctg tctgctgaga
    31021 aatcgttgaa gcaccgcgaa tacggttttc attgcgctcg ttgtgcgcca gggcttgctc
    31081 aatggaagcg acatcaaagc cccagtgctc aggaaatttc tgatcttctg cggcaatcac
    31141 cgccagtccc atccacggcg agatttgatc catactgacc cagtcagaat gtgccacgta
    31201 acgaaaattg ccatgcagcc aggcgctgac ttgccgctcg accattaccg ctgagaaagg
    31261 aacaggcgca acgctaaaca acgcgatgcc cccgccccag aaaatggcga ggacaaccat
    31321 taaccgcaga agaaaacggc gaacgaaact aaacaccgtt aagcggcttt tactcattca
    31381 gccagaacca gcacgcggga aaccagctta tcaatgccgc tcgccgcttc tgcaatgttc
    31441 tgcgccagca tatatgccgg ggtggtgaca atcttattgt cttcatcaac cacgatatca
    31501 tcgacaggac acggcacatg ctccgcgccc atctcttcca gcacttctgc ggtatcgata
    31561 tcagtaccga tggtcaaacg cagcgggaaa tcgaaaattt tcggcagtat cgccggtgct
    31621 atacacataa aaccaagcgg ttttccggcc tgatgcatcg cttgtgccag cgcctttaat
    31681 tcacggtcaa cggtgcattc gctaccaaga ctggcaaaat tgcttaaatt cttcgccgcg
    31741 ccaaaacccc ccggcacaat caacgcatcc agttcagcgg catcggcctg ggccagagga
    31801 cggatttcac cgcgcgttat tcgtgccgct tcaatcagca cattgcgcgt ttccgtcatc
    31861 gcttcaccag ttaaatggtt gataacatca acctgctgct tatccggtgc aaagcagacc
    31921 gcctgcgcac cgctgcgtga aatagctaac aacgtcaaca ccgcttcatg aatttcagaa
    31981 ccgtcataga cgccgcatcc gctcagaatt acgccaattt tcttcattgt gatcttcctt
    32041 tcgcaattga ctgaaacaca ttaatttttt taataaaaat gctacgcatc acacatttaa
    32101 ctgattcatg taacaaatca tttaagtttt gctatcttaa ctgcgtgcgg cctgaaaaac
    32161 agtgctgtgc ccttgtaact catcataata atttacggcg cagccaagat ttccctggtg
    32221 ttggcgcagt attcgcgcac cccggtctag ccggggtcat tttttagtgg cttttgccac
    32281 ccacgctttc agtacttcta cgtcgtgacg ccactcttct ttcatctctt caatccattc
    32341 accgacgtta tcttcccagg cgggaaggtc aggagactga atttgctgac ccagctgttg
    32401 cagatggcgt aaccccactg accccgccgc acctttaatt ttatgccctt cctcaacaat
    32461 gcctttttta tcctgcgccg tcagattcga ctccagcacg ctgacatagc ccggcatcat
    32521 cctctcaaac accgctaacc cgtcggtgat cagcttcggt cctacaagtt cgagatactg
    32581 ttccagcatg ggaatatcga gcaatgcttc tgatttactg ttctcttctg tcgtcaccgt
    32641 actctcctca tcatcctggg tatcccagaa tttcttgatc atcgcggtta gcgccggaac
    32701 agaaagcggc ttactcagca catcatccat tccagcattg aggtactctt gtttgtcttt
    32761 cagcacgtta gcggttaagg ccaccagcgg cggtaaatcc tcgcgcggat aacgcttcgt
    32821 cagttcacga gagatatcca gcccggtcat atctggcaac tgaatatcca gcaacaccag
    32881 gtcgtattcg cccggtttaa acatctccag cgccgccttg ccggtcatgg cgacatcaac
    32941 gctgttacct aacttttcca gcacagagcg cgcgacaatc acgttcagtt caatgtcttc
    33001 caccagcagc acattcagcg ccggtaaagg catatcgtct tcatcaaacg catcatcaac
    33061 ctcttctgct accgacggtg cgtggatcgt caacgtaaag gttgaacctt tgccctgttc
    33121 gctggtaacc gtaatatcgc cgcccatatt tttcgccaga cgacgagaaa cggccagacc
    33181 aataccggtg ccggtggcag gtttaccgcc atgactgtct ttcacctggt aatacatggc
    33241 aaagatttta tccagctcat cctgcggaat gccgatacca gagtcttcca cttcaaaatg
    33301 cagcatatcg ccttcatcgt agcgcacgcg cacggtaacc tggccttgct gagtgaattt
    33361 gacggcgtta ctgatgaggt tccacaagat ctgccgtaaa cgcgtcccgt cggtaatgac
    33421 ctgatgcggt aatggcagcg tcggctccag attaaagcgc aatccttttt gttgcgcctg
    33481 caatgcggag agattttcca gatcggcaag gaagctggtg aaatcaaccg gttgattatc
    33541 aagctggacc ttgcgccgtt ccatcttatc catgtcgata atatcgttga agatattccc
    33601 cagcgtgaca gccgaaacat ggatggtttt gagatatttt tcctgctcgg cggtgagttc
    33661 agtatccagc agaatgcggc tcaggccgac gataccgttc agcggtgtac gcaattcgtg
    33721 actgatggtg gagataaacg tcgttttgtc gcggctggcg cgttcaagcg catcctgata
    33781 ccgcttacgc tcggtaatgt cgcgaccaaa gcccatcaaa ccgtgacgtt tacccacgcg
    33841 gtcgtagtac ggtactttac ggatttcaaa gcaggctttg cgtccgtcgg gataatccag
    33901 ccactgctca taggtcagtg acacattatg acggaacact ttttcatcgg tttcaatgac
    33961 tttcgcagcg gcttccggtg agtaaacatc agcaggtttc aggtgaacca gttgtttttc
    34021 gctttttccg gtcagcagct ccatcgcgcg gttacagccg gaaaactctt tatcttcgtt
    34081 acgataaaaa accaggtcgg gtgaagcgtc aaggaaggaa cgtaagaatg aggattgctg
    34141 ctcgagctga atttgtgtct cttcgcgctc tttgatttca attttcagtt ggccgaaggt
    34201 ttcctgcagt tctgcttccg ctttttcacg aacggcgatt tcctgattta gctgggcaat
    34261 attatctttt aactgaacgt tgaggctcaa atcgcgctcg cgcatctcct ccagtttttg
    34321 caccagtcgt gacagacgtt gtcgtgactc ctccagttgc tcgacgacca ccgatagaaa
    34381 gtagaccgcc cacggcgtaa tcagcaaacc aaagaagata gaacgaataa catcaatgct
    34441 ttcgacctgg ccatgcagca ccatggttac cgccatttgt accacaatgg caagaacgac
    34501 gagggccagc gccagcaaca ttgagaagcg caccagacct aacttcatca tcaggtcaac
    34561 ataatactgc gccagcagac gaatttgctt cattagggaa ttccttcacg acaacctgcc
    34621 ccaataatac ccaattctga cagttacgtt ataaattgtg tgagaaatgc ggaattcccc
    34681 tcaatggcct acgccgttgg aggtagccac ggacgtccca gcgccgatcc ctgtacgcca
    34741 tgttcattca gataacgatc cagctcgacc attcccgtcc atcgattttc gcaccacagc
    34801 ggagcaagca gcgtcgggcg acgggcgctg gcggaaatgc ggtggtagat cacttccggt
    34861 ggcgtatggc gaatcatttc tccggcagtg agcgtgtaat cctccagttc aattccgttc
    34921 aaacgccccg cttcccaggc tttcgccata atgctgcctt tcactatatg tagcggatgc
    34981 agctttatgc cgtccacgcc ggtttcaacc actcgctcca gcgtttgcaa acattcagcc
    35041 tgcccttcgc caggcaggcc aacaattaag tgagaacaca ccttcagccc acgctcacgc
    35101 gccagctgcg tcgtgcgctg atagcaggca aaatcatgac cgcgattgat gcgatgcagt
    35161 gttttgtcgt gggcggtttg tagccccagc tccagccaca cttcgtagcc ctggtcctta
    35221 tattcgcaaa gcagatccag caccgcatcc ggcacgcagt ccgggcgggt accaacacac
    35281 aaaccgacaa tattggcctg gctcaccgcc tgctgataca tcgaacgcag cacctgaact
    35341 tccgcaaagg tgctggtata cgcctgaaag taggccagat agcgtttagc gcggttaact
    35401 aaattcgcct ggtgcgccag ttgctcggca atggaacgat gctgctgcgc ttcatcggca
    35461 aacgaggcaa cattacagaa tgtgcagccg ccacgcccga tggtaccgtc acggttaggg
    35521 cagctaaaac cgccatgcag cgtcagctta tgcacctttt gcccataacg acgggtgaga
    35581 tcaccaccaa acatattgac taatttctgt aactgcataa tctgatagac cgcgccttga
    35641 aaagaggcca aagcctgcca tttttagccc aattcggcga tgacctggat caatcgtccc
    35701 ggcccgcttt tatcaaatgc ataataaatc aaaattaccg aaatttcatg cacaatcaca
    35761 taaatcactt ttgcttatct tgtgtcagat ttttttatct ccttatggat tttaggcaaa
    35821 aacagtagca tgaaacgtta ttaccaatta aggcagtata aaatgctggc tttctcgtca
    35881 attcaaggca ggataagggt taacacgcct ttatgacagt caggaactg
//
LOCUS       DADSYM010000029        35730 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2415
            SAMN08039579-rid11723173.denovo.029, whole genome shotgun sequence.
ACCESSION   DADSYM010000029 DADSYM010000000
VERSION     DADSYM010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039579
            Sequence Read Archive: SRR6362533
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 35730)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35730)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980162.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 03:39:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,484
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 4,347
            CDSs (with protein)               :: 4,347
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 43 of 85
            Pseudo Genes (incomplete)         :: 37 of 85
            Pseudo Genes (internal stop)      :: 19 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35730
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039579-rid11723173.denovo.029"
                     /strain="LGE2415"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8302 N 95.9628 W"
                     /collection_date="2015-08-24"
     gene            157..441
                     /gene="pduA"
                     /locus_tag="J1Y83_001211"
     CDS             157..441
                     /gene="pduA"
                     /locus_tag="J1Y83_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001183614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propanediol utilization microcompartment protein
                     PduA"
                     /protein_id="HBA7629377.1"
                     /translation="MQQEALGMVETKGLTAAIEAADAMVKSANVLLVGYEKIGSGLVT
                     VIVRGDVGAVKAATDAGAAAARNVGEVKAVHVIPRPHTDVEKILPKGINP"
     gene            438..1250
                     /gene="pduB"
                     /locus_tag="J1Y83_001212"
     CDS             438..1250
                     /gene="pduB"
                     /locus_tag="J1Y83_001212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005228567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propanediol utilization microcompartment protein
                     PduB"
                     /protein_id="HBA7629378.1"
                     /translation="MSSNELVEQIMAQVIARVATPENEVLPKTTHQIRETAMAEKSCT
                     LTEFVGTAIGDTVGLVIANVDSALLDAMKLEKRYRSIGILGARIGAGPHIMAADEAVK
                     ATNTEVISIELPRDTKGGAGHGSLILLGGNDVSDVKRGIEVALKELDRTFGDVYANEA
                     GHLELQYTARASYALEKAFGAPLGRACGVIVGAPAAVGVLMADTALKSANVDVVAYSS
                     PAQGTSYSNEVILIISGDSGAVRQAVISAREIGKTVLSTLGDTPKNDRPSYI"
     gene            1269..2933
                     /gene="pduC"
                     /locus_tag="J1Y83_001213"
     CDS             1269..2933
                     /gene="pduC"
                     /locus_tag="J1Y83_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propanediol dehydratase large subunit PduC"
                     /protein_id="HBA7629379.1"
                     /translation="MRSKRFEALAKRPVNQDGFVKEWIEEGFIAMESPNDPKPSIKIV
                     NGVVTELDGKPANQFDLIDHFIARYGINLARAEEVIAMDSVKLANMLCDPNVKRSDIV
                     PLTTAMTPAKIVEVVSQMNVVEMMMAMQKMRARRTPSQQAHVTNIKDNPVQIAADAAE
                     GAWRGFDEQETTVAVARYAPFNAIALLVGSQVGRPGVLTQCSLEEATELKLGMLGHTC
                     YAETISVYGTEPVFTDGDDTPWSKGFLASSYASRGLKMRFTSGSGSEVQMGYAEGKSM
                     LYLEARCIYITKAAGVQGLQNGSVSCIGVPSAVPSGIRAVLAENLICSSLDLECASSN
                     DQTFTHSDMRRTARFLMQFLPGTDFISSGFSAVPNYDNMFAGSNEDAEDFDDYNVIQR
                     DLKVDGGLRPVREEDVIAIRNKAARALQAVFAGMGLPPITDEEVEAATYAHGSKDMPE
                     RNIVEDIKFAQEIINKNRNSLEVVKALAQGGFTDVAQDMLNMQKAKLTGDYLHTSAII
                     VDDGQVLSAVNDINDYAGPATGYRLQGERWEEIKNIPGALDPNEID"
     gene            2943..3611
                     /locus_tag="J1Y83_001214"
     CDS             2943..3611
                     /locus_tag="J1Y83_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004016448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propanediol/glycerol family dehydratase medium
                     subunit"
                     /protein_id="HBA7629380.1"
                     /translation="MEINEKLLRQIIEDVLAEMQTSDKSVSFRAPVSATVSSVPDTEN
                     FLTEIGEAQQGTQQDEVIIAVGPAFGLAQTVNIIGIPHKNILREVIAGIEEEGIKARV
                     IRCFKSSDVAFVAVEGDRLSGSGIAIGIQSKGTTVIHQQGLPPLSNLELFPQAPLLTL
                     DTYRQIGKNAARYAKRESPQPVPTLNDQMARPKYQAKSAILHIKETKYVVTGKKPQEL
                     RVTF"
     gene            3626..4144
                     /gene="pduE"
                     /locus_tag="J1Y83_001215"
     CDS             3626..4144
                     /gene="pduE"
                     /locus_tag="J1Y83_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propanediol dehydratase small subunit PduE"
                     /protein_id="HBA7629381.1"
                     /translation="MNTDAIESMVRDVLNRMNSLQDAVPVSAVPSASIQSAKVTDYPL
                     ANKHPEWVKTATNKTLDDFTLENVLSDNVTAQDMRITPETLRIQAAIARDAGRDRLAM
                     NFERAAELTSVPDDRILEIYNALRPYRSTKQELIAIADDLERRYQAQICAAFVREAAE
                     LYVERKKLKGDD"
     gene            4154..5986
                     /locus_tag="J1Y83_001216"
     CDS             4154..5986
                     /locus_tag="J1Y83_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diol dehydratase reactivase subunit alpha"
                     /protein_id="HBA7629382.1"
                     /translation="MRYIAGIDIGNSSTEVALATLSATGELSFVSSALAETTGIKGTL
                     RNVHGIQEALAQATKKVGINVSDISLIRINEATPVIGDVAMETITETIITESTMIGHN
                     PKTPGGVGLGVGLTITPQELLTCPADTPYILVVSSAFDFADIATMINASVRAGYQLTG
                     VILQRDDGVLVNNRLEIPLPIVDEVLYIDRIPLGMLAAIEVAVPGKVIETLSNPYGIT
                     TVFALNAEETKNIVPVARALIGNRSAVVVKTPSGDVKARSIPAGNIELLSAGRTTRVD
                     VAAGADAIMKAVGECPKLENVTGEPGSNIGGMLEHVRQTMAELTNKPSNEIFIQDLLA
                     IDTSVPVNVTGGLAGEFSLEQAVGIASMVKSDRLQMAMIASEIKQKLHVDVQVGGAEA
                     EAAIQGALTTPGTTRPLAILDLGAGSTDASIINQKGEIVATHLAGAGDMVTMIIAREL
                     GLNDRYLAEEIKKYPLAKVESLFHLRHEDGSVQFFPTPLSPHVFARVCVVKPDELVPI
                     PGDLTLEKVRAVRRSAKERVFVTNALRALRQVSPAGNIRDIPFVVLVGGSSLDFEVPQ
                     LVTDALAHYRLVAGRGNIRGSEGPRNAVATGLLIAWHKESIHGK"
     gene            5976..6326
                     /locus_tag="J1Y83_001217"
     CDS             5976..6326
                     /locus_tag="J1Y83_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000377971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propanediol dehydratase"
                     /protein_id="HBA7629383.1"
                     /translation="MESKERAPAIVVMEIGDCITTWDEVLLGIEEEGIPFRIQHIPSG
                     EVIDSAWLAARQSPLLVGIACDQEKLIVHYKNLPASAPLFTLMYQQDTHARRSIGTNA
                     ARLVKGIPFRELHS"
     gene            6346..6621
                     /gene="pduJ"
                     /locus_tag="J1Y83_001218"
     CDS             6346..6621
                     /gene="pduJ"
                     /locus_tag="J1Y83_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propanediol utilization microcompartment protein
                     PduJ"
                     /protein_id="HBA7629384.1"
                     /translation="MNNALGLVETKGLVGAIEAADAMVKSANVQLVGYEKIGSGLITV
                     MVRGDVGAVKAAVDAGSAAASAVGEVKSCHVIPRPHSDVEAILPKSA"
     gene            6645..7067
                     /locus_tag="J1Y83_001219"
     CDS             6645..7067
                     /locus_tag="J1Y83_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003844245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMC domain-containing protein"
                     /protein_id="HBA7629385.1"
                     /translation="MKQSLGLLEVSGLALAISCADAMAKAASITLVGLEKTNGSGWTL
                     IKITGDVASVNAAIATGASFAGQRNGLVAHKVIARPGDGILPHTTEALTVTVETSETP
                     ATTVISEIISCNLCLDPACQRQKGEPRSLCLHANKRGE"
     gene            7070..7702
                     /locus_tag="J1Y83_001220"
     CDS             7070..7702
                     /locus_tag="J1Y83_001220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000361033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate propanoyltransferase"
                     /protein_id="HBA7629386.1"
                     /translation="MDKQTLQETVSQILDEMRERPIPLGVSSRHIHLSTADYLRLFPE
                     QPISEKKPLMQPGQFAAEQVVTLEGPKGKLHNVRLLGPLRNASQVEISRTDARTLGIT
                     APLRMSGDLSGTPGIRLISPFAELEITSGVIVAQRHIHMSPLDALIYGVTHGDKVAVA
                     IKGDERRLIFENVAVRVSPDMRLEMHIDTDEANAAGADSPDVFATLVSVP"
     gene            7699..8190
                     /gene="pduM"
                     /locus_tag="J1Y83_001221"
     CDS             7699..8190
                     /gene="pduM"
                     /locus_tag="J1Y83_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001061905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcompartment protein PduM"
                     /protein_id="HBA7629387.1"
                     /translation="MNNMLMQRIVDEVVFRLKQRAGKTLVLTVFQLRDASVQEIVHQY
                     ASLQIRYVDLPLLRQLAGNETSDRAAIQIHEALAWGLHIQLSLQRHFLNAIELKTLAR
                     LPLSWCDEQGQPIYLHRDRLLSYADIAQLSSGILVLQRKCCVTALAREAAITRNIQLI
                     RQE"
     gene            8194..8469
                     /gene="pduN"
                     /locus_tag="J1Y83_001222"
     CDS             8194..8469
                     /gene="pduN"
                     /locus_tag="J1Y83_001222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005228577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propanediol utilization microcompartment protein
                     PduN"
                     /protein_id="HBA7629388.1"
                     /translation="MHLARVTGAVVSTQKSPSLIGKKLLLVRRVSADGELPLTPLTGD
                     EVAVDSVGAGVGELVLLSSGSSARHVFSGPNEAIDLAVVGIVDTLSR"
     gene            8481..9488
                     /gene="pduO"
                     /locus_tag="J1Y83_001223"
     CDS             8481..9488
                     /gene="pduO"
                     /locus_tag="J1Y83_001223"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-domain cob(I)yrinic acid a,c-diamide
                     adenosyltransferase PduO"
                     /protein_id="HBA7629389.1"
                     /translation="MAIYTRTGDSGSTSLFTGQRVSKTHLRVETYGTLDELNATLSLC
                     YCATAIESHRILLEAIQQQIFWFSAELASESEQPSAQQRYIGTEEIAALENAIDSAMN
                     AVPPVHCFILPGRCEAASRMHFARTVARRAERRLVELTTETTIRNVLLHYINRLSDCL
                     YALARVEDNVAHQNLMIQEITKRYHEANHTPALKERTMPLTFQDLHQLIRSAAMRADE
                     LHIPVVISIVDANGTESVTWRMPDALLVSSELAPKKAWTAVAMKTATHKLADTVQPGA
                     PLYGLESHMQGKVVTFGGGFPLWRDGKLLGGLGISGGSVEQDMDIAQSAMAAINVGVN
                     Q"
     gene            9485..10870
                     /gene="pduP"
                     /locus_tag="J1Y83_001224"
     CDS             9485..10870
                     /gene="pduP"
                     /locus_tag="J1Y83_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-acylating propionaldehyde dehydrogenase
                     PduP"
                     /protein_id="HBA7629390.1"
                     /translation="MNNSELETLIRNILREQLVPATPRMQRNAIFQTVDEAVCAAHQA
                     FLRFQQCPLKTRSAIISAIREELMPRLTELAEESAKETGMGNKEDKYLKNKAALDNTP
                     GIEDLTTTALTGDGGMVLFEYSPFGVIGSVTPSTNPTETIINNSISMLAAGNSVYFSP
                     HPGAKNVSLKLIAIIEDIAFRCSGIHNLIVTVSEPTFAATEQMMAHPKISVLAITGGP
                     GIVAMGMKSGKKVIGAGAGNPPCIVDETADIVKAAEDIINGASFDYNLPCIAEKSLIV
                     VASVADYLIQQMQSFGALLLNHEQTEKLRAICLPDGSANKKLVGKSPSALLEAAGLPL
                     PAKASRLLIAVVDADDSWVTCEQLMPMLPIVKVNDFDSALALALKVEDGLHHTAIMHS
                     QNVSRLNLAARVMQTSIFVKNGPSWAGIGVGGEGFTTFTIATPTGEGTTSARTFARSR
                     RCVLTSGFSIR"
     gene            10881..11993
                     /locus_tag="J1Y83_001225"
     CDS             10881..11993
                     /locus_tag="J1Y83_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001091448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HBA7629391.1"
                     /translation="MKTFSLQTRLYSGTGSLNILSRFTNRHIWIICDVFLAHSPLIDT
                     LHQALPDSNRLSIFSEITPDPTIQTVVKGIAQMQTLRPDVVIGFGGGSALDAAKAIVW
                     FGRQCGIEIETCVAIPTTSGTGSEVTSACVISDPEKGIKYPLFDNALYPDIAILDPSL
                     VVSVPPAITANTGMDVLTHALEAYVSPRASDFTDALVEKAVQIVFQYLPTAVKKGDCL
                     ATRGKMHNASTLAGIAFSQAGLGLNHAIAHQLGGQFHLPHGLANALLLTAVIRFNAGD
                     PRAAKRYTRLAKTCHLCPDNANDTASLNALIQHIEQLKTTCTLPTLTNALKEKKAEWS
                     IRIPDMVQAALADATLRTNPRAADASAIAELLEELL"
     gene            11990..13333
                     /locus_tag="J1Y83_001226"
     CDS             11990..13333
                     /locus_tag="J1Y83_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001585845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex protein RnfC"
                     /protein_id="HBA7629392.1"
                     /translation="MNVGLISCDDQTIRERVQAAGVVGAGGAGFPTHIKLQARVDTFL
                     VNAAECEPMLKVDQQLMVQQASRLLRGVHYAMKATGASSGIIALKEKYQRAINALTPL
                     LPPDIRLHILPDVYPAGDEVLTIWMATGRRVPPAALPVSVGVVVNNVQTVLNIARAVE
                     QQYPVTRRTLTINGAVAKPITVTVPIGMSLREVLALAGGATVDAPGFINGGPMMGSLI
                     TSLDTPVSKTTGGLLVLPNSHSLIQRRMQNDRSVLTVAKTVCEQCRLCTDLCPRHLVG
                     HELAPHLLVRAVNYQQLATPQLLLTALTCSECNVCASVACPVGISPMRINRLLKQELR
                     AQNLRYEGALNPADPMANYRLIPVKRLVTRLGLIPWYQDAPLSEQVPQPEKVTLLLRQ
                     HIGASAIACVQKGDRVVHGQCIGQIPHGTLGAPIHASIDGMVSDVTENAITLVRG"
     gene            13336..13890
                     /gene="pduT"
                     /locus_tag="J1Y83_001227"
     CDS             13336..13890
                     /gene="pduT"
                     /locus_tag="J1Y83_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propanediol utilization microcompartment protein
                     PduT"
                     /protein_id="HBA7629393.1"
                     /translation="MSKAVGILELSSIAKGMETGDAMLKSANVELLVSKTLCPGKFLL
                     MLGGDVGAVQQAINSGASLAGEMLVDSLVLPNIHPSLLSAISGLNHVEQHRAVGVVET
                     WSVAACIDAADCAVKAANVILVRVHMAFGIGGKCYMVVAGDISDVNNAVSVASERAGE
                     KGLLVYRSVIARPHEAMWRQMVEG"
     gene            13890..14240
                     /gene="pduU"
                     /locus_tag="J1Y83_001228"
     CDS             13890..14240
                     /gene="pduU"
                     /locus_tag="J1Y83_001228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000441103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propanediol utilization microcompartment protein
                     PduU"
                     /protein_id="HBA7629394.1"
                     /translation="MTTQHTTERMIQEYVPGKQVTLAHVIANPGKDLFKKLGLPDTVS
                     AIGILTITPSEASIIACDIATKSGAVEIGFLDRFTGAVVLTGDVSAIEYALSQVTRTL
                     GEMMRFTACPITRT"
     gene            14245..14688
                     /gene="eutP"
                     /locus_tag="J1Y83_001229"
     CDS             14245..14688
                     /gene="eutP"
                     /locus_tag="J1Y83_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001585847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EutP/PduV family microcompartment system
                     protein"
                     /protein_id="HBA7629395.1"
                     /translation="MQRMMLIGPSQCGKTSLTQCLRGENIHYQKTQAIVWSPTTIDTP
                     GEYLENRSLYSALLVSACEADIIALVLNADAPWSPFSPGFTSSMNRPVIGVVTKADLA
                     SMEQISLVKCWLQEAGAHNVLVTSAVNNNGVTELFALLHTEEGCR"
     gene            complement(14789..15118)
                     /locus_tag="J1Y83_001230"
     CDS             complement(14789..15118)
                     /locus_tag="J1Y83_001230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310836.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF496 family protein"
                     /protein_id="HBA7629396.1"
                     /translation="METTKPSFQDVLEFVRLFRRKNKLQREIQDVEKKIRDNQKRVLL
                     LDNLSDYIKPGMSVEAIQGIIASMKGDYEDRVDDYIIKNAELSKERRDISKKLKAMGE
                     MKNGEAK"
     gene            complement(15290..16348)
                     /locus_tag="J1Y83_001231"
     CDS             complement(15290..16348)
                     /locus_tag="J1Y83_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HBA7629397.1"
                     /translation="MRADKSLSPFEIRVYRHYRIVHGTRVALAFLLTFLIIRLFTIPE
                     STWPLVTMVVIMGPISFWGNVVPRAFERIGGTVLGSILGLIALQLELISLPLMLVWCA
                     AAMFLCGWLALGKKPYQGLLIGVTLAIVVGSPTGEIDTALWRSGDVILGSLLAMLFTG
                     IWPQRAFIHWRIQLAKSLTEYNRVYQSAFSPNLLERPRLESHLQKLLTDAVKMRGLIA
                     PASKETRIPKSIYEGIQTINRNLVCMLELQINAYWATRPSHFVLLNAQKLRDTQHMMQ
                     QILLSLVHALYEGNPQPVFANTEKLNDAVEELRQLLDNHHDLKVVETPIYGYVWLNME
                     TAHQLELLSNLICRALRK"
     gene            complement(16546..17019)
                     /gene="sbmC"
                     /locus_tag="J1Y83_001232"
     CDS             complement(16546..17019)
                     /gene="sbmC"
                     /locus_tag="J1Y83_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor SbmC"
                     /protein_id="HBA7629398.1"
                     /translation="MNYEIKQEDKRTVAGFHLVGPWEQTVKKGFEQLMMWIDSKNIVP
                     KEWVAVYYDNPDETPAEKLRCDTVVTVPNNFTLPENSEGVILTEISGGQYAVAVARVV
                     GDDFAKPWYQFFNSLLQDSAYEMLPKPCFEVYLNNGAEDGYWDIEMYVAVQPKHH"
     gene            complement(17138..18304)
                     /gene="dacD"
                     /locus_tag="J1Y83_001233"
     CDS             complement(17138..18304)
                     /gene="dacD"
                     /locus_tag="J1Y83_001233"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase DacD"
                     /protein_id="HBA7629399.1"
                     /translation="MKRRLIIAASLFVFNLSSGFAAENIPFSPQPPEIHAGSWVLMDY
                     TTGQILTAGNEHQQRNPASLTKLMTGYVVDRAIDSHRITPDDIVTVGRDAWAKDNPVF
                     VGSSLMFLKEGDRVSVRDLSRGLIVDSGNDACVALADYIAGGQRQFVEMMNNYAEKLH
                     LKDTHFETVHGLDAPGQHSSAYDLAVLSRAIIHGEPEFYHMYSEKSLTWNGITQQNRN
                     GLLWDKTMNVDGLKTGHTSGAGFNLIASAVDGQRRLIAVVMGADSAKGREEEARKLLR
                     WGQQNFTTVQILHRGKKVGTERIWYGDKENIALGTEQEFWMVLPKAEIPHIKAKYTLD
                     GKELTAPISAHQRVGEIELYDRDKQVAHWPLVTLESVGEGSMFSRLSDYFHHKA"
     gene            18513..19940
                     /gene="sbcB"
                     /locus_tag="J1Y83_001234"
     CDS             18513..19940
                     /gene="sbcB"
                     /locus_tag="J1Y83_001234"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease I"
                     /protein_id="HBA7629400.1"
                     /translation="MMNDGKQQSTFLFHDYETFGTHPALDRPAQFAAIRTDNEFNVIG
                     EPEVFYCKPADDYLPQPGAVLITGITPQEARAKGENEAAFAARIHSLFTVPKTCILGY
                     NNVRFDDEVTRNVFYRNFYDPYAWSWQHDNSRWDLLDVMRACYALRPEGINWPENDDD
                     LPSFRLEHLTKANGIEHSNAHDAMADVYATIAMAKLVKTRQPRLFDYLFTHRNKHKLM
                     ALIDVPQMKPLVHVSGMFGAWRGNTSWVAPLAWHPENRNAVIMVDLAGDISPLLELDS
                     DTLRERLYTAKADLGDNAAVPVKLVHINKCPVLAQANTLRPEDADRLGINRQHCLDNL
                     KILRENPQVREKVVAIFAEAEPFTPSNNVDAQLYNGFFSDADRAAMKIVLETEPRNLP
                     ALDITFVDKRIEKLLFNYRARNFPGTLDYAEQQRWLEHRRQVFTPEFLQGYADELQML
                     AQQYADDKEKVALLKALWQYAEEIV"
     gene            complement(20014..21372)
                     /gene="plaP"
                     /locus_tag="J1Y83_001235"
     CDS             complement(20014..21372)
                     /gene="plaP"
                     /locus_tag="J1Y83_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416518.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine/proton symporter PlaP"
                     /protein_id="HBA7629401.1"
                     /translation="MSHNVTPNTSRVELRKTLTLVPVVMMGLAYMQPMTLFDTFGIVS
                     GLTDGHVPTAYAFALIAILFTALSYGKLVRRYPSAGSAYTYAQKSISPTVGFMVGWSS
                     LLDYLFAPMINILLAKIYFEALVPSIPSWMFVVALVAFMTAFNLRSLKSVANFNTVIV
                     VLQIVLIAVILGMVVYGVFEGEGAGTLASTRPFWSGDAHVIPMITGATILCFSFTGFD
                     GISNLSEETKDAERVIPRAIFLTALIGGMIFIFATYFLQLYFPDISRFKDPDASQPEI
                     MLYVAGKAFQVGALIFSTITVLASGMAAHAGVARLMYVMGRDGVFPKSFFGYVHPKWR
                     TPAMNIILVGAIALLAINFDLVMATALINFGALVAFTFVNLSVISQFWIREKRNKTLK
                     DHFQYLFLPMCGALTVGALWVNLEESSMVLGLIWAAIGLIYLACVTKSFRNPVPQYED
                     VA"
     gene            complement(21362..21424)
                     /gene="yoeI"
                     /locus_tag="J1Y83_001236"
     CDS             complement(21362..21424)
                     /gene="yoeI"
                     /locus_tag="J1Y83_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein YoeI"
                     /protein_id="HBA7629402.1"
                     /translation="MGQFFAYATVITVKENDHVA"
     gene            complement(21639..22568)
                     /locus_tag="J1Y83_001237"
     CDS             complement(21639..22568)
                     /locus_tag="J1Y83_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416519.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBA7629403.1"
                     /translation="MKPLLDVLMILDALEKEGSFAAASAKLYKTPSALSYTVHKLESD
                     LNIQLLDRSGHRAKFTRTGKMLLEKGREVLHTVRELEKQAIKLHEGWENELVIGVDDT
                     FPFSLLAPLIEAFYQHHSVTRLKFINGVLGGSWDALTQGRADIIVGAMHEPPSSSEFG
                     FSRLGDLEQVFAVAPHHPLALEEEPLNRRIIKRYRAIVVGDTAQAGASTASQLLDEQE
                     AITVFDFKTKLELQISGLGCGYLPRYLAQRFLDSGALIEKKVVAQTLFEPVWIGWNEQ
                     TAGLASGWWRDEILANSAIAGVYAKSDDGKSAI"
     gene            complement(22614..23438)
                     /locus_tag="J1Y83_001238"
     CDS             complement(22614..23438)
                     /locus_tag="J1Y83_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HBA7629404.1"
                     /translation="MKKVAIVGLGWLGMPLAMSLSARGWQVTGSKTTQDGVEAARMSG
                     IDSYLLRMEPELVCDSDDLDALMDADALVITLPARRSGPGDEFYLQAVQELVDSALAH
                     RIPRIIFTSSTSVYGDAQGTVKETTPRNPVTNSGRVLEELEDWLHNLPGTSVDILRLA
                     GLIGPGRHPGRFFAGKTAPDGEHGVNLVHLEDVIGAITLLLQAPKGGHIYNICAPAHP
                     ARNVFYPQMARLLGLEPPQFRNSLDSGKGKIIDGSRICNELGFEYQYPDPLVMPLE"
     gene            complement(23521..23775)
                     /gene="yoeB"
                     /locus_tag="J1Y83_001239"
     CDS             complement(23521..23775)
                     /gene="yoeB"
                     /locus_tag="J1Y83_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     toxin YoeB"
                     /protein_id="HBA7629405.1"
                     /translation="MKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGK
                     PEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAACRYHY"
     gene            complement(23772..24023)
                     /gene="yefM"
                     /locus_tag="J1Y83_001240"
     CDS             complement(23772..24023)
                     /gene="yefM"
                     /locus_tag="J1Y83_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoeB-YefM toxin-antitoxin system antitoxin YefM"
                     /protein_id="HBA7629406.1"
                     /translation="MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEE
                     YNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKDIIE"
     gene            24306..24356
                     /gene="hisL"
                     /locus_tag="J1Y83_001241"
     CDS             24306..24356
                     /gene="hisL"
                     /locus_tag="J1Y83_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="his operon leader peptide"
                     /protein_id="HBA7629407.1"
                     /translation="MTRVQFKHHHHHHHPD"
     gene            24502..25401
                     /gene="hisG"
                     /locus_tag="J1Y83_001242"
     CDS             24502..25401
                     /gene="hisG"
                     /locus_tag="J1Y83_001242"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HBA7629408.1"
                     /translation="MTDNTRLRIAMQKSGRLSDDSRELLARCGIKINLHTQRLIAMAE
                     NMPIDILRVRDDDIPGLVMDGVVDLGIIGENVLEEELLNRRAQGEDPRYFTLRRLDFG
                     GCRLSLATPVDEAWDGPLSLNGKRIATSYPHLLKRYLDQKGISFKSCLLNGSVEVAPR
                     AGLADAICDLVSTGATLEANGLREVEVIYRSKACLIQRDGEMEESKQQLIDKLLTRIQ
                     GVIQARESKYIMMHAPTERLDEVIALLPGAERPTILPLAGDQQRVAMHMVSSETLFWE
                     TMEKLKALGASSILVLPIEKMME"
     gene            25407..26711
                     /gene="hisD"
                     /locus_tag="J1Y83_001243"
     CDS             25407..26711
                     /gene="hisD"
                     /locus_tag="J1Y83_001243"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HBA7629409.1"
                     /translation="MSFNTIIDWNSCTAEQQRQLLMRPAISASESITRTVNDILDNVK
                     TRGDEALCEYSAKFDKTTVTALKVSADEITAASERLSDELKQAMAVAVKNIETFHTAQ
                     KLPPVDVETQPGVRCQQVTRPLASVGLYIPGGSAPLFSTVLMLATPARIAGCKKVVLC
                     SPPPIADEILYAAQLCGVQDVFNVGGAQAIAALAFGTESVPKMNKIFGPGNAFVTEAK
                     RQVSQRLDGAAIDMPAGPSEVLVITDSGATPDFVASDLLSQAEHGPDSQVILLTPDAD
                     MARRVAEAVERQLAELPRAETARQALNASRLIVTKDLAQCVEISNQYGPEHLIIQTRN
                     ARELVDGITSAGSVFLGDWSPESAGDYASGTNHVLPTYGYTATCSSLGLADFQKRMTV
                     QELSKEGFSTLASTIETLAAAERLTAHKNAVTLRVNALKEQA"
     gene            26708..27778
                     /gene="hisC"
                     /locus_tag="J1Y83_001244"
     CDS             26708..27778
                     /gene="hisC"
                     /locus_tag="J1Y83_001244"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HBA7629410.1"
                     /translation="MSTVTITDLARENVRNLTPYQSARRLGGNGDVWLNANEYPTAVE
                     FQLTQQTLNRYPECQPKVVIENYAQYAGVKPEQVLVSRGADEGIELLIRAFCEPGKDA
                     ILYCPPTYGMYSVSAETIGVECRTVPTLDNWQLDLQGISDKLDGVKVVYVCSPNNPTG
                     QLINPQDFRTLLELTRGKAIVVADEAYIEFCPQASLAGWLAEYPHLAILRTLSKAFAL
                     AGLRCGFTLANEEVINLLMKVIAPYPLSTPVADIAAQALSPQGIVAMRERVAQIITER
                     EYLIAALKEIPCVEQVFDSETNYILARFKASSAVFKSLWDQGIILRDQNKQPSLSGCL
                     RITVGTREESQCVIDALRAEQV"
     gene            27778..28845
                     /gene="hisB"
                     /locus_tag="J1Y83_001245"
     CDS             27778..28845
                     /gene="hisB"
                     /locus_tag="J1Y83_001245"
                     /EC_number="3.1.3.15"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     histidinol-phosphatase/imidazoleglycerol-phosphate
                     dehydratase HisB"
                     /protein_id="HBA7629411.1"
                     /translation="MSQKYLFIDRDGTLISEPPSDFQVDRFDKLAFEPGVIPELLRLQ
                     KAGYKLVMITNQDGLGTQSFPQADFDGPHNLMMQIFTSQGVQFDEVLICPHLPADECD
                     CRKPKVKLVERYLAEQAMDRANSYVIGDRATDIQLAENMGINGLRYDREALNWPMIGE
                     QLTKRDRYAHVVRNTKETQIDVQVWLDREGGSKINTGVGFFDHMLDQIATHGGFRMEI
                     NVKGDLYIDDHHTVEDTGLALGEALKIALGDKRGICRFGFVLPMDECLARCALDISGR
                     PHLEYKAEFTYQRVGDLSTEMIEHFFRSLSYTMGVTLHLKTKGKNDHHRVESLFKAFG
                     RTLRQAIRVEGDTLPSSKGVL"
     gene            28845..29435
                     /gene="hisH"
                     /locus_tag="J1Y83_001246"
     CDS             28845..29435
                     /gene="hisH"
                     /locus_tag="J1Y83_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HBA7629412.1"
                     /translation="MNVVILDTGCANLNSVKSAIARHGYEPKVSRDPDVVLLADKLFL
                     PGVGTAQAAMDQVRERELFDLIKACTQPVLGICLGMQLLGRRSEESNGVDLLGIIDED
                     VPKMTDFGLSLPHMGWNRVYPQAGNRLFQGIEDGAYFYFVHSYAMPVNPWTIAQCNYG
                     EPFTAAVQKDNFYGVQFHPERSGAAGAKLLKNFLEM"
     gene            29435..30172
                     /gene="hisA"
                     /locus_tag="J1Y83_001247"
     CDS             29435..30172
                     /gene="hisA"
                     /locus_tag="J1Y83_001247"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310852.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HBA7629413.1"
                     /translation="MIIPALDLIDGTVVRLHQGDYGKQRDYGNDPLPRLQDYAAQGAK
                     VLHLVDLTGAKDPAKRQIPLIKTLVAGVNVPVQVGGGVRSEEDVATLLEAGVARVVVG
                     STAVKSPEMVKGWFERFGADALVLALDVRIDEQGNKQVAVSGWQENSGVSLEQLVETY
                     LPVGLKHVLCTDISRDGTLAGSNVSLYEEVCARYPQVAFQSSGGIGDINDVAALRGTG
                     VRGVIVGRALLEGKFTVKEAIACWQNA"
     gene            30154..30930
                     /gene="hisF"
                     /locus_tag="J1Y83_001248"
     CDS             30154..30930
                     /gene="hisF"
                     /locus_tag="J1Y83_001248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015853929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HBA7629414.1"
                     /translation="MLAKRIIPCLDVRDGQVVKGVQFRNHEIIGDIVPLAKRYAEEGA
                     DELVFYDITASSDGRVVDKSWVSRVAEVIDIPFCVAGGIKSLEDAAKILSFGADKISI
                     NSPALADPTLITRLADRFGVQCIVVGIDTWYDAETGKYNVNQYTGDESRTRVTQWETL
                     DWVQEVQKRGAGEIVLNMMNQDGVRNGYDLEQLKKVREVCHVPLIASGGAGTMEHFLE
                     AFRDADVDGALAASVFHKQIINIGELKAYLATQGVEIRIC"
     gene            30924..31535
                     /locus_tag="J1Y83_001249"
     CDS             30924..31535
                     /locus_tag="J1Y83_001249"
                     /EC_number="3.5.4.19"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoribosyl-AMP
                     cyclohydrolase/phosphoribosyl-ATP diphosphatase HisIE"
                     /protein_id="HBA7629415.1"
                     /translation="MLTEQQRRELDWEKTDGLMPVIVQHAVSGEVLMLGYMNPEALDK
                     TIESGKVTFFSRTKQRLWTKGETSGNFLNVVNIAPDCDNDTLLVLANPIGPTCHKGTS
                     SCFGDTAHQWLFLHQLEQLLAERKSADPETSYTAKLYASGTKRIAQKVGEEGVETALA
                     ATVHDRFELTNEASDLMYHLLVLLQDQDLDLTTVIENLRKRHQ"
     gene            complement(31721..32698)
                     /gene="wzzB"
                     /locus_tag="J1Y83_001250"
     CDS             complement(31721..32698)
                     /gene="wzzB"
                     /locus_tag="J1Y83_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310855.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS O-antigen chain length determinant protein
                     WzzB"
                     /protein_id="HBA7629416.1"
                     /translation="MRVENNNVSGQNHDPEQIDLIDLLVQLWRGKMTIIISVIVAIAL
                     AIGYLVVAKEKWTSTAIVTQPDVGQIAGYNNAINVIYGSAAPKVSEIQSMLIGRFSTT
                     FSALAETLDNQEEPEKLTIEPTVKNQSLPLAVSYVGETPEGAQKQLAKYIQQVDDQVN
                     EELEQDLKDNIALQMKNLQDSLKTQEVVAQEQKDLRIRQIQEALQYANQAQVTKPQIQ
                     QTQDVTQDTMFLLGSEALESMIKHEATRPLVFSSNYYQTRQNLLDIDNLDVDKLDIHA
                     YRYVMKPTLPIRRDSPKKAITLILAVLLGGMVGAGIVLGRNALRNYNAK"
     gene            complement(32845..34011)
                     /gene="ugd"
                     /locus_tag="J1Y83_001251"
     CDS             complement(32845..34011)
                     /gene="ugd"
                     /locus_tag="J1Y83_001251"
                     /EC_number="1.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 6-dehydrogenase"
                     /protein_id="HBA7629417.1"
                     /translation="MKITISGTGYVGLSNGILIAQNHEVVALDIVQAKVDMLNKKQSP
                     IVDKEIEEYLATKDLNFRATTDKYDAYKNADYVIIATPTDYDPKTNYFNTSSVEAVIR
                     DVTEINPNAVMVIKSTIPVGFTKSIKERLGIDNLIFSPEFLREGKALYDNLHPSRIVI
                     GERSERAERFAALLQEGAIKQNIPTLFTDSTEAEAIKLFANTYLAMRVAYFNELDSYA
                     ESLGLNTRQIIEGVCLDPRIGNHYNNPSFGYGGYCLPKDTKQLLANYQSVPNNLISAI
                     VDANRTRKDFIADAILSRKPQVVGIYRLIMKSGSDNFRASSIQGIMKRIKAKGVEVII
                     YEPVMKEDSFFNSRLERDLATFKQQADVIISNRMAEELKDVADKVYTRDLFGSD"
     gene            complement(34260..35666)
                     /gene="gndA"
                     /locus_tag="J1Y83_001252"
     CDS             complement(34260..35666)
                     /gene="gndA"
                     /locus_tag="J1Y83_001252"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000043504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HBA7629418.1"
                     /translation="MSKQQIGVVGMAVMGRNLALNIESRGYTVSIFNRSREKTEEVIA
                     ENPGKKLVPYYTVKEFVESLETPRRILLMVKAGAGTDAAIDSLKPYLDKGDIIIDGGN
                     TFFQDTIRRNRELSAEGFNFIGTGVSGGEEGALKGPSIMPGGQKEAYELVAPILTKIA
                     AVAEDGEPCVTYIGADGAGHYVKMVHNGIEYGDMQLIAEAYSLLKGGLNLTNEELAQT
                     FTEWNNGELSSYLIDITKDIFTKKDEDGNYLVDVILDEAANKGTGKWTSQSALDLGEP
                     LSLITESVFARYISSLKDQRVAASKVLSGPQAQPAGDKAEFIEKVRRALYLGKIVSYA
                     QGFSQLRAASEEYNWDLNYGEIAKIFRAGCIIRAQFLQKITDAYAENPQIANLLLAPY
                     FKQIADDYQQALRDVVAYAVQNGIPVPTFAAAVAYYDSYRAAVLPANLIQAQRDYFGA
                     HTYKRIDKEGVFHTEWLD"
BASE COUNT         9096 a         9195 c         8961 g         8478 t
ORIGIN      
        1 caaaacaacc aataaattaa caacatatag ttcataaaag caattttatt atatttgttc
       61 tctattaatt tttctcatta gccgattaag tcagccattc tttttcctcg ctttttttta
      121 tagtcatatc gtcaaaaaaa tccaggcgag gtctttatgc aacaagaagc gttaggaatg
      181 gtagaaacca aaggcttaac tgccgccata gaggccgcag acgccatggt gaagtcggca
      241 aatgttttgc tggtcggcta cgaaaaaata ggttccgggc tggtgaccgt catcgttcgt
      301 ggtgatgtcg gggcggtaaa agcggcaaca gacgcgggtg ccgcagctgc gcgtaacgtc
      361 ggcgaagtaa aagccgtaca cgttattccc cgcccacata ccgatgtcga aaaaatctta
      421 ccaaagggaa ttaacccatg agcagcaatg aactggtcga acagatcatg gcgcaggtca
      481 tcgctcgcgt agcgacgcca gagaatgagg ttctcccgaa aaccactcat caaatacgag
      541 agacggctat ggcagaaaaa agctgcacat taacagaatt tgttggcacc gctattggcg
      601 acactgtagg tctggtcatt gcgaacgttg atagcgccct gttggacgcg atgaaacttg
      661 aaaaacgcta tcgttccatt ggcattctgg gggcgcgtat tggcgcaggg ccacacatta
      721 tggccgctga tgaagcggta aaagccacca acactgaagt tattagcatt gaacttcctc
      781 gcgatactaa aggcggtgcc ggacacggct cattgatcct cttaggtggc aacgatgttt
      841 ccgacgtgaa acgcggtatt gaagttgccc tgaaagaact cgatcggacc tttggtgatg
      901 tctatgccaa cgaagccggg catcttgaac tgcaatacac cgcccgcgcc agctatgcac
      961 tggaaaaagc gtttggcgct ccactgggac gggcttgcgg cgttattgtc ggcgctccgg
     1021 ctgcggtggg tgttctaatg gccgacaccg cgctgaagtc cgccaatgtg gatgtcgtgg
     1081 catacagctc ccccgctcaa ggcaccagct acagcaacga agtcattctg attatctcgg
     1141 gtgactccgg cgcagtccgc caggcggtca tctccgcacg cgaaatcggt aaaaccgtgc
     1201 tttcgactct cggcgataca ccgaaaaacg atcgcccttc ctacatctga attctctgag
     1261 gcagatttat gagatcgaaa agatttgaag cactggcaaa acgcccggtc aaccaggacg
     1321 gtttcgttaa agagtggatt gaagaaggct tcattgcgat ggaaagcccg aacgatccta
     1381 aaccgtcaat caaaattgtt aacggcgtcg ttaccgaact ggatggaaaa cccgccaatc
     1441 agtttgacct gatcgaccac tttatcgccc gctacggtat caatctcgcg cgcgcagaag
     1501 aagtcatcgc gatggattcg gtaaaacttg ccaatatgtt gtgcgatcca aatgtcaaac
     1561 gtagcgatat cgttcccctt accacagcaa tgacgcctgc aaaaatcgtc gaagtggttt
     1621 cgcagatgaa cgtggtagag atgatgatgg cgatgcagaa aatgcgcgcc cgtcgaacac
     1681 catcacaaca ggctcacgtc accaacatca aagataaccc ggtacaaatt gccgccgacg
     1741 cagctgaagg cgcatggcgc gggtttgatg aacaggaaac caccgttgcc gttgctcgct
     1801 atgcaccgtt caacgccatc gccctgctgg tcggctcaca ggtgggacgt ccgggcgtct
     1861 tgacccagtg ttcactggaa gaagccaccg aactgaaact cggtatgtta ggccacacct
     1921 gttacgccga aacaatttct gtctatggta cagaacctgt tttcaccgat ggcgatgata
     1981 ccccgtggtc aaaaggattt ctcgcgtcat cctatgcttc tcgcggctta aaaatgcgct
     2041 ttacttccgg ctccggctct gaagtacaga tgggttacgc ggaaggcaaa tcaatgcttt
     2101 atctcgaggc tcgatgtatt tacatcacca aagccgcagg cgttcagggt ctgcagaatg
     2161 gttcggtaag ctgcatcggt gttccatcag ctgtgccatc aggcatccgt gcagtattag
     2221 cggaaaactt aatttgctca tcgctggatc tggaatgcgc ctccagtaat gaccagacct
     2281 ttacccactc ggatatgcgc cgtaccgcac gtttcctgat gcaatttctg ccaggtaccg
     2341 actttatctc ttccggtttt tccgctgtgc cgaactacga caacatgttt gctggttcaa
     2401 acgaagatgc tgaagacttc gatgattaca acgtcattca gcgtgacctg aaggttgatg
     2461 gcgggctgcg accagtacgc gaagaagatg ttatcgcaat ccgcaataaa gccgctcgcg
     2521 ctttacaggc tgtctttgcc ggtatgggac taccgccaat taccgatgaa gaagtcgaag
     2581 cggcaaccta tgcccacggt tcgaaagata tgcctgagcg caatattgtg gaagacatca
     2641 agttcgccca ggagattatc aataaaaacc gtaacagcct ggaagtggtg aaagccctgg
     2701 cgcaaggcgg ttttaccgat gtcgcccagg acatgctcaa catgcaaaaa gcaaaactga
     2761 ccggcgatta tctccatacc tcagccatca tcgtcgatga cggacaagtg ctctcagcgg
     2821 tcaatgacat caatgattac gccggaccag ctacaggtta ccgcctgcaa ggggaacgct
     2881 gggaagagat caaaaatatt cccggtgcac ttgatcccaa cgaaattgac taagggtgag
     2941 aaatggaaat taacgaaaaa ttactgcgtc agattatcga agacgtgctg gcagaaatgc
     3001 aaaccagcga caagtccgtt tcatttcgcg cacctgtctc tgcaactgtc tcttcagtgc
     3061 cagataccga aaattttctg accgaaattg gcgaagccca acaaggcact cagcaagatg
     3121 aagtcattat tgctgtaggc ccggcgttcg gcctggcgca aaccgtcaat atcatcggta
     3181 ttccgcataa aaacattttg cgcgaagtga ttgccggtat cgaagaagaa ggcattaaag
     3241 cccgtgtgat tcgctgcttt aaatcgtccg atgtcgcatt cgttgcagtc gaaggagatc
     3301 gtcttagtgg ttcaggtatt gctatcggta ttcagtcgaa aggcacaacg gttatccacc
     3361 agcagggact tcctcccttg tctaacctgg aacttttccc acaagcgcct ttgttgacgc
     3421 tggatactta tcgtcagatt ggcaaaaacg ccgcgcgtta tgcgaagcgc gagtctccac
     3481 aaccggtgcc gaccctcaac gatcaaatgg cgcggccgaa ataccaggcc aaatctgcca
     3541 ttttgcatat taaagagacg aagtacgtgg tcactggcaa aaaaccgcaa gaactgcgcg
     3601 tgacgtttta acaaaggaaa ccttcatgaa taccgatgca attgagtcga tggtcaggga
     3661 cgtgttaaac cgcatgaaca gtttacagga tgcagttcct gtttctgcgg tccccagcgc
     3721 gtcaatacag agcgccaaag tgacggatta cccgctggca aataaacatc cggaatgggt
     3781 taaaacagca accaataaaa cgctcgacga tttcactctg gaaaatgtgc tcagcgataa
     3841 cgtcaccgcc caggatatgc gcattacccc tgaaacattg cgtattcagg ccgcgattgc
     3901 cagagatgct ggtcgtgatc gcctggcgat gaatttcgaa cgcgccgctg agcttacctc
     3961 agtaccggat gatcgcatcc tcgaaatcta caatgcgtta cgcccttatc gttcgactaa
     4021 gcaagaactt attgccattg ctgacgatct tgaacggcgt tatcaggccc aaatttgtgc
     4081 tgcatttgtc cgtgaagctg cggaacttta tgtcgagcgc aaaaaactga aaggcgacga
     4141 ttaaggacga attatgcgat atatagcagg cattgatatc ggcaactcat caacagaggt
     4201 tgcgctggca acgctcagtg caactggaga gttgtcgttc gtgagtagcg ccctggcgga
     4261 aaccaccgga attaaaggta cgttgcgtaa cgtccatggt attcaggagg cgctcgcaca
     4321 ggcaacgaaa aaagtcggca tcaatgttag cgatatttcg cttattcgta tcaacgaagc
     4381 aactccggtc attggtgatg tggcgatgga aaccatcact gaaactatca tcaccgaatc
     4441 aaccatgatc ggtcacaacc cgaaaactcc gggcggagtg gggctgggcg tagggctcac
     4501 catcactccg caagagttat taacatgccc ggcggacaca ccctacattc tggtggtgtc
     4561 atcggcattt gattttgccg atatcgcaac gatgataaac gcctccgtac gagccggata
     4621 tcagctgact ggcgttattc tgcaacgcga cgatggcgta ctggtcaata accgcctgga
     4681 aattcctctc cctattgttg atgaagtcct ctacatcgat cgtattccgc tcggcatgct
     4741 ggctgcaatt gaagttgctg ttcccgggaa agttatcgaa accctgtcta acccgtatgg
     4801 gattaccacc gttttcgccc tcaatgctga agagactaaa aatattgttc ctgtcgcgcg
     4861 ggcgctaatc ggcaaccgtt cagcagtggt agtgaaaacg ccttctggcg acgttaaagc
     4921 ccgttcgata cccgccggaa atatcgaact gctttctgcc gggcgcacaa cgcgggttga
     4981 tgtagctgct ggtgccgatg ccattatgaa agcggtcggt gagtgcccaa aactggaaaa
     5041 cgtgacggga gaacctggct ccaacatcgg cggaatgctg gaacatgttc gccagaccat
     5101 ggctgaactg accaacaaac ccagcaacga aatttttatt caggatttgc tggcgataga
     5161 tacctctgtc cccgtcaacg taaccggtgg tctggcaggt gaattttcac tggaacaggc
     5221 tgtgggtatt gcctctatgg tgaaatccga ccgtctgcaa atggcgatga tcgccagtga
     5281 aattaagcaa aaactgcatg ttgatgttca ggtcggaggc gcagaagcag aagccgccat
     5341 tcagggggcc ttgactaccc ctggaacgac acgtccactg gcaattctgg acttaggtgc
     5401 aggctctacc gatgcctcca tcatcaatca gaaaggggaa atagttgcca ctcaccttgc
     5461 gggagcgggc gacatggtaa cgatgattat cgctcgtgag ctgggcttaa atgatcgtta
     5521 tctggcagaa gaaatcaaaa agtacccgtt ggcaaaagta gaaagcctgt tccacttacg
     5581 ccatgaagat ggcagcgttc agttcttccc tactccccta tcccctcatg tatttgcccg
     5641 cgtttgtgtg gtgaaacctg acgaactggt gcctattccc ggtgatttga cgctggaaaa
     5701 agtacgcgct gtccgtcgca gtgcgaaaga gcgtgtcttc gtaactaatg ccctgcgtgc
     5761 gctgcgtcag gtaagccctg ccggaaatat tcgcgacatt ccgttcgtcg ttctggtagg
     5821 tggctcttcg ctggatttcg aagtaccgca attggtcacc gacgcgctgg cacattatcg
     5881 actggtcgcc gggcggggga atatccgtgg tagcgaaggt ccgcgcaatg cggtagctac
     5941 tgggctcctc atcgcctggc acaaggagtc gattcatgga aagtaaagag cgtgcaccag
     6001 ccatcgttgt catggagatc ggtgactgta tcactacatg ggatgaagtg cttcttggca
     6061 ttgaagagga aggtattccc tttcgcattc aacacattcc gtcaggtgaa gttatcgata
     6121 gcgcctggct ggctgctcgt caatctcccc tgttggttgg cattgcctgc gatcaggaaa
     6181 aactgattgt gcattacaaa aacttaccgg catcagcgcc actgtttacg ctgatgtatc
     6241 aacaagatac ccatgccagg cgcagcatcg gcactaacgc tgcccgcctg gtaaaaggga
     6301 ttcccttccg ggaactccac tcataatcca caggagaaac gcagtatgaa caacgcacta
     6361 ggactggttg aaacaaaagg gttagttggc gcaattgaag ctgcagatgc catggttaaa
     6421 tccgccaacg tgcagctggt tggctatgaa aagattggtt cgggtctgat tacggtgatg
     6481 gtccgtggcg atgttggcgc agtaaaagcc gccgttgatg caggaagcgc cgccgcaagc
     6541 gccgttggcg aagtgaaatc ctgccatgtc atcccgcgcc cacacagtga tgttgaggct
     6601 attttaccta aatcagcata accccattcg ctaaggagca cagcgtgaag caatcactgg
     6661 gattacttga agtcagtggt ctggcactcg ccatcagttg cgcagatgct atggcaaaag
     6721 ccgcgtccat cacgctggtt ggccttgaga agaccaatgg ctcaggctgg acgctcatca
     6781 aaatcaccgg tgacgtcgcc tcggttaatg cggcgatcgc gacgggagcc agtttcgccg
     6841 gacagcgaaa tggcctcgtc gcacacaaag tgattgcccg tccgggagac ggcattctgc
     6901 cccacacaac tgaagcattg actgtgacgg ttgaaacaag cgaaacacca gcaacaaccg
     6961 tcatctcaga gattatcagt tgtaatttgt gtcttgaccc ggcatgtcag cggcaaaaag
     7021 gtgagccccg ctcactttgc ctgcacgcca ataagcgagg ggagtaacag tggataaaca
     7081 aaccttgcag gaaacagtga gccagattct ggatgagatg cgcgagcgcc ctatccctct
     7141 gggtgtttcc agtcgtcata ttcatctttc aacagcggat tatttgcgtc tttttcctga
     7201 acaaccgatt agcgagaaaa agccgttaat gcaacctggc caattcgctg ctgaacaggt
     7261 tgttacgctg gagggaccaa aaggtaaatt gcacaacgtt cgcttgttgg ggccgctacg
     7321 caatgcaagc caggtggaaa tttcccgtac ggacgccaga acgttaggga tcaccgcccc
     7381 gctacgcatg tcaggcgatc tcagcggcac tccagggatt cgtttgataa gcccatttgc
     7441 ggaactggag atcacatctg gcgtcattgt cgcacaacgg catattcata tgtctccgct
     7501 ggatgccctg atttatggcg ttacgcatgg cgataaagtg gcggttgcca ttaaaggtga
     7561 tgagcgacga ctgatattcg aaaacgtcgc tgttcgcgtc tctccggata tgcgtcttga
     7621 aatgcatatt gataccgatg aagccaacgc cgcaggggct gatagccctg acgtttttgc
     7681 cacactggtg tcagtgccat gaataacatg ctgatgcaac gtattgttga cgaagtggtt
     7741 tttcgcctta aacaacgtgc gggaaaaaca ctcgtgctga ctgtttttca actgcgagac
     7801 gccagcgttc aagagatagt tcatcagtat gcatcattac aaatccgata tgtcgatctg
     7861 ccgctgctgc gacaacttgc ggggaacgaa acgtcagatc gagcagcaat tcagatacac
     7921 gaagcgttag cctggggatt gcacattcaa ttgtcgttgc agcgtcattt cctgaacgcc
     7981 attgaattga agactctcgc ccgtctaccg ctaagctggt gcgacgagca agggcaaccg
     8041 atctaccttc atcgtgatcg acttttaagt tatgccgata ttgcacagtt aagctcggga
     8101 atactggtgt tacagcgaaa atgttgtgtg accgcactgg ctcgtgaagc ggcaattacc
     8161 cgaaatattc agttaatcag gcaggagtaa cctatgcatc tggctcgagt cactggcgcg
     8221 gtagtatcca cgcaaaaatc accttctctg atcgggaaaa aactgctgct ggttcgccgg
     8281 gtcagtgctg acggcgaact gcctctcacg ccattaaccg gcgacgaagt tgccgttgat
     8341 tcagtcggcg caggtgttgg tgagttggta ctcctcagca gtggttccag cgccagacat
     8401 gtgttttccg gccctaacga agccatcgac ctggcagtgg tcggcattgt cgatacgctt
     8461 tcgcgctaag gagctgaaag atggcaattt atacccgaac aggtgattca ggaagtacgt
     8521 ctttatttac cggacagcgc gtcagcaaaa ctcatctacg tgtcgaaacc tatggcacgc
     8581 tggatgaact gaacgcgaca ctcagtctgt gttattgcgc caccgccatc gaaagccatc
     8641 gcatcttact ggaagccatt caacaacaga tattttggtt tagcgccgaa ctcgccagtg
     8701 aaagcgaaca gccgtcagcg cagcagcgtt acatcggtac ggaagaaatt gccgcgctgg
     8761 aaaacgcgat cgatagcgca atgaacgccg tccccccggt tcactgcttc attctgcctg
     8821 gtcgatgtga agccgcaagc cgcatgcatt tcgcccgcac ggtggctcga cgggctgaaa
     8881 gacgtctggt tgaactgaca acggaaacca ccatccggaa tgttttgctg cactatatca
     8941 accgactttc cgattgcctg tatgccctcg cccgtgtgga ggacaatgtt gctcatcaaa
     9001 acctgatgat tcaggaaatc acaaaacgtt atcacgaagc caaccacaca ccagcattga
     9061 aggaacgcac aatgccgcta acttttcagg atcttcacca gctcattcgt agtgcggcaa
     9121 tgcgcgcaga tgaactgcat attcccgttg tcatcagcat tgttgatgcc aacggaacag
     9181 aaagtgttac ctggcggatg cctgatgccc tgttggtcag cagtgaactg gcaccgaaaa
     9241 aagcctggac cgccgtggcg atgaaaacgg caacccacaa acttgctgat accgtgcaac
     9301 ccggcgcgcc gctttacggg ctcgaaagcc atatgcaagg caaagtcgtc acttttggtg
     9361 gcggcttccc cctctggcgt gacggaaaat tgcttggcgg gcttggcatc agcggcggta
     9421 gcgttgaaca agacatggat attgcccaaa gcgcaatggc ggcaattaac gtgggagtaa
     9481 accaatgaat aactctgaat tagaaacact gatccgcaat attctgcgtg aacaacttgt
     9541 ccccgctaca ccacgaatgc aaagaaacgc tattttccag actgttgatg aagcggtctg
     9601 cgcagcacac caggcctttt tacgctttca acagtgcccg cttaaaaccc gtagcgccat
     9661 tatcagcgcc attcgcgaag aattaatgcc ccgccttacc gaactggcag aagaaagcgc
     9721 caaagaaacg gggatgggaa ataaagaaga taagtaccta aaaaacaaag cggcactcga
     9781 taatacgcca ggaattgaag atttaaccac cactgcacta accggtgatg gtggcatggt
     9841 tttgtttgaa tactccccgt ttggggttat tggctcggtc acgccgagta ccaaccccac
     9901 ggaaaccatc attaacaaca gcatcagcat gctggcagca ggtaatagcg tctacttcag
     9961 tccacacccg ggagctaaaa acgtatcgct gaaactgatt gccataatcg aagacatcgc
    10021 gtttcgctgt agtggcattc acaacctgat cgttaccgta tctgaaccaa cctttgccgc
    10081 caccgaacag atgatggcgc accctaaaat cagcgttctg gcgattaccg gcggcccggg
    10141 gatcgtcgcc atgggaatga aaagtggcaa gaaagtgatt ggtgccggag cgggtaaccc
    10201 accctgcatt gtcgatgaaa cagcggatat tgtgaaagcc gccgaggaca tcatcaacgg
    10261 cgcttcattt gattacaacc tgccgtgtat cgctgaaaaa agcctgattg tcgtggcatc
    10321 agtggcagat tatttaattc agcagatgca aagctttggc gcacttcttc ttaaccatga
    10381 acaaacggag aaattgcgtg caatttgcct gccagacggt agcgccaaca aaaaactggt
    10441 tgggaaaagc ccatcagcct tgctggaagc agcgggcctc cctttgcccg caaaagcgtc
    10501 gcgcctactg attgctgttg ttgacgccga tgatagttgg gttacgtgtg aacaactgat
    10561 gccaatgctg cccattgtga aggtcaacga ttttgacagc gctctcgccc tggcgttgaa
    10621 agtggaagac ggcttgcacc acacggcaat tatgcactcg cagaacgtct cacggctcaa
    10681 ccttgccgcg cgcgttatgc aaacctcaat ctttgtcaaa aacggcccct cctgggcggg
    10741 tattggcgtc ggcggcgaag gctttacgac ctttaccatt gcaacaccga ccggagaggg
    10801 tactacgtca gcacggactt ttgcccgctc acgacgctgc gtgttaacca gcggcttttc
    10861 cattcgctag agaaacggaa atgaaaactt tttcactgca gacgcgttta tacagcggaa
    10921 cgggaagtct caacatactg agccgtttca cgaataggca catctggatt atttgcgacg
    10981 tctttctggc acactcacca ctcattgaca cgttgcatca ggcgctgcca gacagtaatc
    11041 gtctcagtat ttttagcgaa ataacgcccg acccaactat ccagaccgtg gtgaaaggca
    11101 ttgcgcaaat gcagacttta cgcccggatg tggttatcgg ttttggcggc ggttctgccc
    11161 tggacgccgc aaaagcgatt gtctggttcg gtcgccagtg tggcattgaa attgaaacct
    11221 gcgtagccat tccaaccacc agcggtaccg gttccgaagt gaccagtgct tgtgtcatca
    11281 gcgatcctga gaaagggatt aaatatcccc ttttcgataa tgcactttat ccggatatcg
    11341 cgatccttga tccatctctg gtcgtcagcg tgccaccggc gataacggcc aataccggga
    11401 tggatgtttt aacccatgcg cttgaagctt atgtttctcc ccgcgccagc gacttcacgg
    11461 atgcactggt cgaaaaagcc gtgcaaattg tttttcaata cctgcccact gcagtgaaga
    11521 aaggcgattg tcttgccacc cgtggcaaaa tgcataatgc atcgacgctt gcaggaatag
    11581 cctttagcca ggccggactc ggtttaaacc atgcaattgc tcatcaactc ggcggacagt
    11641 ttcatctgcc gcacggcctg gcgaatgcgc tcctgctcac ggcggttatc cgctttaatg
    11701 ctggcgatcc gcgcgcagcc aaacgctaca cccggctggc gaaaacgtgc catttatgcc
    11761 cggacaacgc caatgacacc gccagcctga atgcacttat tcaacatatt gagcagctta
    11821 aaacaacctg tacgctgccg actcttacca atgcgctgaa agaaaaaaaa gcagaatggt
    11881 caatacgcat accagatatg gttcaggcgg cactggcgga tgcaacgttg cgtaccaacc
    11941 cgcgtgcggc tgatgcctcc gctattgcag aactgctcga ggagttgtta tgaacgtcgg
    12001 attaatctcc tgcgacgacc agaccatccg cgagcgagta caggcagcag gtgtcgtggg
    12061 tgctggcggg gcaggttttc caacccatat taagctacaa gcgcgtgttg acacgttcct
    12121 ggtgaatgcg gcggaatgcg aaccaatgtt gaaagtggat caacagttaa tggtgcagca
    12181 agccagccgc ctgcttcgcg gtgttcacta tgctatgaaa gccaccgggg ccagtagcgg
    12241 aatcatcgcg ctgaaagaga aatatcaacg ggcaataaac gcactcacac cactgttacc
    12301 accggatatc cgcctgcata ttttgcccga tgtttacccc gcaggagacg aagtgctgac
    12361 catctggatg gcaactggtc gccgcgttcc acccgctgca ttacccgtta gcgtcggtgt
    12421 ggtggtcaat aatgttcaaa ccgtacttaa tattgccaga gccgtcgagc aacagtatcc
    12481 agtgacgcgc cgcacactca ctattaatgg tgcagtggcg aaaccaataa cggtaaccgt
    12541 ccccatcggc atgtctttac gtgaagtcct ggcgctggcg ggcggcgcaa cagtcgatgc
    12601 gcctggtttt atcaatggcg ggccgatgat gggaagtttg atcacatcgc tcgacacacc
    12661 cgtcagcaaa accactggcg gcttgctggt tttacccaac tctcattcac tgatccaacg
    12721 tcgaatgcag aatgaccgca gcgtgcttac cgtcgcgaaa actgtttgtg agcaatgccg
    12781 cttgtgtacc gatctctgcc cacgtcattt agtcggtcac gaactggcac cccatttatt
    12841 agtacgtgcg gttaactacc aacagctcgc tacgccacaa ctgttactga cggcgctcac
    12901 ctgttcggag tgcaacgtct gcgccagcgt cgcctgtccg gtaggaattt caccaatgcg
    12961 cataaaccgc ctgcttaagc aggaattacg cgcacaaaat ctccgctacg aaggtgcctt
    13021 aaatcctgcg gatcccatgg caaattatcg actgattccc gtaaaacggc tggtaaccag
    13081 actgggccta atcccgtggt atcaggatgc gccattgtct gaacaggtgc ctcagcccga
    13141 aaaagtgacg ttattgttac gccagcacat cggtgccagc gcaattgcgt gtgtacaaaa
    13201 aggcgatcgc gtcgttcatg ggcaatgtat aggtcaaatt ccacacggca cattaggtgc
    13261 cccgattcat gccagtattg atggcatggt cagcgatgtg acagaaaacg cgattacgct
    13321 tgtgagaggt taacgatgtc gaaagcggta ggtattttag aacttagcag tatcgcgaaa
    13381 gggatggaaa ccggcgacgc catgctgaaa agcgcaaacg ttgagttact ggtcagcaaa
    13441 acgctatgtc ccggaaaatt tctgctgatg ctcggtggcg atgtgggcgc ggtgcaacag
    13501 gccatcaaca gcggagcgtc ccttgctggt gaaatgctgg tcgacagtct tgtcctgccg
    13561 aatattcatc ccagtctcct ttctgcaatc agcggactta accatgttga acagcaccgg
    13621 gctgtcggcg tagtcgaaac ctggagtgtc gccgcctgca ttgacgctgc cgattgtgct
    13681 gtcaaagctg cgaatgtcat tctcgttcgc gtacatatgg cttttggcat tggtggtaaa
    13741 tgctacatgg tggttgctgg cgatatttca gatgttaata acgcggtctc tgtcgccagc
    13801 gagcgtgcgg gggaaaaagg tttgttggtc tatcggtcag tgattgcccg tccacatgaa
    13861 gccatgtggc gtcagatggt ggagggataa tgacaactca gcacacaact gaacggatga
    13921 ttcaggaata tgtccctggt aagcaggtca cgctggcaca tgtcatcgcc aatccaggga
    13981 aggatctgtt taagaaattg ggtttaccag acaccgtttc tgctattggc attctgacga
    14041 ttacccccag cgaagcatct atcatcgcat gcgatatcgc cacaaaatca ggcgctgtag
    14101 agatcggttt tcttgatcgt tttaccggtg ccgtagtgct gactggtgat gtttctgcta
    14161 ttgaatatgc acttagccag gttacccgta cgctgggtga aatgatgcgc tttaccgcct
    14221 gccccattac gcggacctga cgttatgcaa cgtatgatgc ttatcggccc cagccagtgc
    14281 ggaaaaacgt cactcacgca atgtctgcgt ggcgaaaata tccattatca gaaaacgcag
    14341 gcaatcgtct ggtccccgac caccattgat acaccaggcg aatacctcga aaaccgcagc
    14401 ctctacagtg cattgttggt cagtgcctgc gaagcagaca ttatcgcgct ggtacttaat
    14461 gcggacgcac cgtggtcacc tttttcgcca ggctttacct cgtcaatgaa tcgcccggtc
    14521 attggagtag taaccaaagc agatttagcc agtatggaac aaatatcttt ggtgaaatgt
    14581 tggttacagg aagctggagc gcacaacgtg ttagtaacca gtgcggttaa taacaatgga
    14641 gttacagaac tttttgcctt gctgcataca gaagaaggct gtcgctaatt tcgataggtt
    14701 ctcagatgag aatgatgacg gtgatacaca gataagaatc aatgagcaat gagggttgcc
    14761 gggcaaccct cattgaataa aacaggaatt acttcgcttc gccgtttttc atttcgccca
    14821 tagctttcag ctttttggag atatcgcggc gttctttgga gagctcggca tttttgatga
    14881 tgtaatcgtc aacgcgatct tcatagtcac ctttcatgct ggcgatgatg ccctggattg
    14941 cttcaacgct catccctggc ttgatgtaat cgctcaggtt gtccagcaac aggacgcgct
    15001 tctggttgtc acggatcttt ttctcaacgt cctgaatttc acgttgcagt ttgttcttac
    15061 gacggaacag acgaacaaat tccagtacgt cctggaatga aggcttggta gtttccattt
    15121 ttatacccct gataatgtga gagtcggatt cgtttaagca accgctattc gttagggcca
    15181 acattactca tagctgccgc agatgacaat gcttttatcc cttttcacta tcataccctt
    15241 tatccttgct gaatcgaagc agcagcaaga tgattctgaa gttcaggaat tatttgcgca
    15301 aggcccggca aatcagattc gatagcaatt caagctgatg cgctgtttcc atgttcagcc
    15361 acacataacc atagattggt gtttccacga ctttcaggtc atggtggtta tcgagcaact
    15421 gacgcagctc ttccacagca tcgttcaatt tttccgtatt ggcaaaaacc ggctgcggat
    15481 taccttcgta cagcgcatga acaaggctca gcagtatttg ctgcatcatg tgctgggtat
    15541 cacgaagttt ttgcgcgttc aataacacga aatggctggg gcgcgtggcc cagtatgcat
    15601 tgatttgcaa ctccagcata caaaccagat tgcgattaat ggtctggata ccttcatata
    15661 tcgatttggg aatacgggtt tctttgctgg cgggcgcaat cagcccacgc attttcacgg
    15721 catcggtcag gagtttttgt agatggcttt ccagacgtgg gcgttcgagt aagttcggtg
    15781 agaatgcaga ttgatagacc cgattatact cggtcagact tttcgccagt tgaatgcgcc
    15841 agtggatgaa cgcccgttgt ggccagatac cggtaaacaa cattgccagt aaagagccga
    15901 ggatcacatc gccgcttcgc cataacgccg tatcaatttc acctgtcggg gaacccacaa
    15961 caattgccag cgtcacccca atcaataatc cttgatacgg tttcttgccc agcgccagcc
    16021 aaccgcaaag aaacatcgcc gccgcgcacc agactaacat cagcggtaac gaaattaact
    16081 ccagttgcag agcgataaga cctaaaatcg accccaacac cgtaccgcca atacgttcga
    16141 aggcacgagg gacaacgttc ccccagaacg agattggccc cataatcacc accatggtga
    16201 ccagcggcca ggtgctttcc gggatagtaa acaggcggat aatgagaaaa gtgagcagga
    16261 atgccagcgc gacccgagta ccatgcacaa tgcggtaatg gcggtatacc cggatttcaa
    16321 acgggcttaa tgacttatcg gcacgcacgc agaaactccg taatgaagat taaaagcgct
    16381 cataacacat ctgtcggcca gaaggcgcgc ctctgagtta tccgacggac ataccgggaa
    16441 cagtgtaatt tcccggcagg agcgaaaaat ttgaaaggcg catcttactc ttttcgcttt
    16501 caaaaaaaga gtggtcttcg cgttaacaca ccgccctgag atgaattagt gatgttttgg
    16561 ctgcaccgca acatacattt cgatatccca gtacccatct tccgcgccat tgttcaagta
    16621 aacctcgaag cagggctttg gtaacatttc ataagcactg tcctgcaaga ggctattaaa
    16681 gaactgatac cagggtttag caaaatcatc accgactaca cgagccaccg ccaccgcata
    16741 ctgaccacct gaaatttctg tcagaatgac gccctcactg ttttcgggga gcgtaaagtt
    16801 attcggtacc gtcacgacgg tgtcgcagcg taatttttcg gcgggtgttt catctggatt
    16861 gtcgtaatag acagcaaccc actccttcgg cacaatattt ttgctatcta tccacatcat
    16921 caactgctca aaacctttct ttaccgtctg ctcccacggg ccaacaagat ggaaacctgc
    16981 aacggtacgt ttatcttcct gcttaatctc gtagttcatg acgcctccac taacactgtt
    17041 tttatataca gtatagttgc aatattaaaa ccacaaggaa tgagtgttga ttatgcgagc
    17101 agactcgcac tcctgccagt ctgctgcaaa agaaaggtca ggccttatgg tggaaataat
    17161 cactcaggcg ggaaaacatg ctgccttccc cgacagattc cagggtaacc agcggccagt
    17221 gcgccacctg tttatcacgg tcgtaaagtt caatttcccc tacccgctga tgggcgctaa
    17281 ttggcgcggt gagttcttta ccatcaaggg tatatttggc tttgatatgt ggaatttcgg
    17341 ctttcggtag caccatccag aactcttgtt ccgttcccag ggcgatattt tctttatcgc
    17401 cataccagat gcgttccgta ccgacctttt tcccacggtg caaaatttgc accgtagtaa
    17461 agttttgttg cccccaacgc agtaattttc ttgcctcttc ctcacgacct tttgcactgt
    17521 cagcacccat taccactgca atgaggcgac gctgcccgtc tacagccgaa gcaataagat
    17581 taaacccggc accagaagta tggcccgttt tcaggccgtc aacattcatg gttttatccc
    17641 acaataaccc gttacggttt tgctgggtga taccgttcca ggtgaggctt ttctcactgt
    17701 acatatgata aaactcgggc tcgccgtgga tgatagcgcg agaaagcacg gctaaatcat
    17761 aagccgagct atgctggcca ggtgcatcca gaccatgcac tgtttcaaaa tgcgtatcct
    17821 tgagatgcag cttctcggca tagttgttca tcatttcaac aaactgccgt tgcccaccgg
    17881 caatatagtc agccagagca acacaagcgt catttccgga atccacaatt aaaccacggc
    17941 ttaaatcacg taccgatacg cgatcgccct ctttcaaaaa catcagtgaa gaaccgacaa
    18001 acaccggatt atctttcgcc cacgcatcac gccccacggt gacaatatcg tctggcgtaa
    18061 tgcgatgact atcgatagcg cgatccacga catagcccgt catcagcttt gtcaggctgg
    18121 cgggattgcg ctgttgatgc tcattacccg cggtgagaat ctgaccggtg gtgtaatcca
    18181 tcaataccca ggacccggca tgaatctctg gaggctgagg tgaaaaagga atgttttccg
    18241 ccgcaaaacc agacgataaa ttaaaaacga acaaagaagc agcaataata agacggcgtt
    18301 tcaacagcaa accctcaggt gtttcaaata gctgttcttt ttacggaaat acttatgaac
    18361 tggctggaat aaagtgcaag aaaatgtgac tactatctca tttttatctg acatgatctg
    18421 ttgccactcg ctgccaaatt gtcgcgctaa agctgattag cacggtgata tttgatactc
    18481 tggcagacag cagaaataac ggatttaacc taatgatgaa tgacggtaag caacaatcta
    18541 cctttttgtt tcacgattac gaaacctttg gaacgcaccc cgcgttagat cgccctgcac
    18601 agttcgcagc cattcgcact gataacgaat tcaatgtcat cggcgaaccc gaagtctttt
    18661 actgcaagcc cgcggatgac tatttacccc agcctggagc agtattaatt accggtatta
    18721 ccccgcagga ggcacgggcg aaaggagaaa acgaagccgc gtttgccgcc cgtattcact
    18781 cgctttttac cgtaccgaag acctgtattc tgggctacaa caatgtgcgt ttcgacgacg
    18841 aagtcacacg caacgttttt tatcgtaatt tctacgatcc ttacgcctgg agctggcagc
    18901 atgataactc gcgctgggat ttactggatg ttatgcgtgc ctgttatgcc ctgcgcccgg
    18961 aaggaataaa ctggcctgaa aatgatgacg atctaccgag ctttcgcctt gagcatttaa
    19021 ccaaagcgaa tggtattgaa catagcaacg cccacgatgc gatggctgat gtgtacgcca
    19081 ctattgcgat ggcgaaactg gtaaaaacgc gtcagccacg cctgtttgat tatctcttta
    19141 cccatcgtaa taaacacaaa ctgatggcgt tgattgatgt tccgcagatg aaacccctgg
    19201 ttcacgtttc cggaatgttt ggggcatggc gcggcaatac cagctgggtg gcaccgctgg
    19261 cgtggcatcc tgaaaatcgc aatgccgtaa ttatggtgga tttggcagga gacatttcgc
    19321 cattactgga gctggatagc gacacattgc gcgagcgttt atataccgca aaagccgatc
    19381 ttggcgataa cgccgccgtt ccggttaagc tggtgcatat caataaatgt ccggtactgg
    19441 cccaggcgaa tacgctacgc ccggaagatg ccgaccgact gggaattaat cgtcagcatt
    19501 gcctcgataa cctaaaaatt ctgcgtgaaa atccgcaagt gcgtgaaaaa gtggtagcga
    19561 tattcgcgga agccgaaccg tttacacctt caaataacgt ggatgcacag ctttataatg
    19621 gctttttcag tgacgccgat cgtgcagcaa tgaaaattgt gctggaaacc gagccgcgta
    19681 atttaccggc actggatatc acttttgttg ataaacgaat tgaaaaactg ttgttcaatt
    19741 atcgagcgcg caacttcccg gggacgctgg attatgccga gcagcaacgc tggctggagc
    19801 accgccgcca ggtcttcacg ccagagtttt tgcagggcta cgctgatgaa ttgcagatgc
    19861 tggcacaaca gtatgccgat gacaaagaga aagtggcgct gttaaaagcg ctttggcagt
    19921 acgcggaaga gatcgtctga aacaaaaaag ccggagcgac aaactccggc tttttgagat
    19981 ttaccggatg attatgtcat ccggtttgta agattacgct acgtcttcgt actgcggaac
    20041 cgggttacgg aaactcttgg ttacgcaagc caggtaaatc agaccgatag cagcccagat
    20101 cagacccaga accattgagc tttcttccag gttaacccac agcgcaccaa cggtcagcgc
    20161 accacacatc ggcaggaaca gatactggaa gtgatctttc agcgtcttgt tacgcttctc
    20221 acggatccag aactgcgaga tgaccgacag gttaacgaag gtgaacgcca ccaacgcacc
    20281 aaagttaatc agcgccgtag ccattaccag gtcgaagttg attgccagca gggcaatcgc
    20341 gccaaccagg atgatgttca tcgccggagt acgccatttc gggtgtacat aaccgaagaa
    20401 gcttttcggg aacacgccgt cacgacccat tacgtacatc agacgcgcta cgcctgcgtg
    20461 cgccgccata ccggacgcca gtacggtaat ggtggagaag atcagcgcgc caacctggaa
    20521 cgctttaccc gcaacgtaca gcatgatttc aggctgtgac gcatccggat ctttaaaacg
    20581 agagatatcc gggaagtaca gctgcaggaa gtaagttgca aagatgaaga tcatgccgcc
    20641 gatcagcgcg gtcaggaaaa tcgcacgcgg gatcacgcgc tctgcatctt tggtttcttc
    20701 cgacaggttg ctgatgccgt caaagccggt aaaggagaag cacaggattg tcgccccggt
    20761 aatcatcggg ataacatgcg catcgccaga ccagaacgga cgagtgctcg ccagcgtacc
    20821 agcgccttca ccttcaaata cgccataaac aaccatgccc agaatcaccg cgatcagcac
    20881 tatctgcaac acgacgatta cagtgttgaa gttcgctacg gatttcagac tacgcaggtt
    20941 aaaggcggtc ataaaggcca ccagcgccac cacaaacatc cacgatggaa tggaaggcac
    21001 cagagcttca aaatagattt tcgccagcag aatgttgatc atcggcgcga acagatagtc
    21061 gagcagagaa gaccaaccca ccataaagcc gacagtcggg ctaatggatt tctgggcgta
    21121 agtgtatgca gagccagcag aaggatagcg gcgaaccagc ttcccgtagc tcagagccgt
    21181 aaacaggatc gcaatcaatg cgaacgcata ggctgtcggc acatgaccat ccgtgaggcc
    21241 tgaaacgata ccaaatgtat caaacagcgt catcggctgc atataggcta gacccatcat
    21301 tacaaccgga actaacgtaa gcgttttacg caattccacg cgagaggtgt ttggagtaac
    21361 gttatgcgac atggtcattc tcctttacgg tgataaccgt cgcgtaagca aaaaattgcc
    21421 ccattttttt ggattcctca gcgacaacaa ctgtcgattt ttagtaaata tctatccggt
    21481 acgaagcccg gcctcttggt atgaatgatt ggtttgaagc aaaaaataac cgacgctaat
    21541 taaacgtcgg tttttagtca ttttttgaca gcggcgcatt gtgcctaaat gggggggaaa
    21601 tgacaagaga atgagaggct tgtcataata atttttcttt aaatggctga ttttccgtca
    21661 tcagattttg cataaacacc ggcgatcgca ctatttgcta aaatttcatc tcgccaccag
    21721 ccgctggcaa gccctgcggt ctgttcattc cagccaatcc agacaggttc aaagagagtt
    21781 tgggcgacca ctttcttctc gattaacgcg ccactatcga gaaagcgttg cgccagatac
    21841 cgtggtaaat agccgcagcc aaggccgcta atttgcagct ccagtttggt tttaaaatcg
    21901 aaaacggtaa tggcttcttg ttcgtcgaga agttgtgagg ccgtagaagc gcctgcctgc
    21961 gcagtgtccc ccaccacaat cgcccgatat cgtttaatga tacgacggtt taacggttct
    22021 tcttccaggg ctaacggatg atgaggcgcg accgcaaaga cttgttccag atcgcccaac
    22081 cgagagaaac caaactcact ggaagatggt ggctcatgca tcgcgccaac aataatatcc
    22141 gctcgcccct gagtcagggc atcccatgaa ccaccgagta cgccattgat aaacttcagg
    22201 cgcgtcacgc tatgatgttg ataaaaagcc tcaatgagtg gcgcaagcag agaaaaaggg
    22261 aaggtgtcgt ccacgccaat caccagctcg ttttcccagc cttcatgcag tttgatcgcc
    22321 tgcttttcca gttctcgcac ggtatgcaga acttcccgcc ctttctctaa taacattttt
    22381 ccggtgcggg tgaatttagc gcggtgtccg ctgcgatcaa gcaattgaat attaaggtcg
    22441 ctttccagct tgtgaacggt gtaactcaga gcggacggcg tcttgtagag tttggccgac
    22501 gccgccgcaa aactgccttc tttttctaag gcatcgagga tcatcaacac atccagcagt
    22561 ggtttcatac acgccccctt gcagtgtatg cggcgtcctg ctggcgtgat cgtttactcc
    22621 agcggcatta ccagcggatc gggatactgg tattcaaatc ccagttcatt acaaatccga
    22681 ctgccatcaa taatcttacc tttgccgctg tccagactat ttctgaactg cggaggttcc
    22741 agccccaata aacgggccat ctgcggatag aaaacattac gcgcagggtg agctggcgca
    22801 catatattat agatgtgtcc gcctttaggt gcctgtaaca acagagtgat agcgccaata
    22861 acatcttcta aatggactaa attaacacca tgttcaccat caggggcggt ttttccggca
    22921 aagaagcgtc cgggatgacg tcccggtccg atcaggcccg caagacgcag aatatcgacc
    22981 gaagtaccgg gtaaattgtg cagccagtct tcgagttctt ctaatactcg tccactgttg
    23041 gtcaccggat tacgcggggt ggtttctttc accgtgcctt gcgcgtcgcc atagacagat
    23101 gtcgagctgg taaaaataat gcggggaata cgatgggcca gcgcgctatc cactaactct
    23161 tgtaccgctt gtaaatagaa ctcatcgcca gggccgctac gacgtgccgg aagcgtaatg
    23221 accagcgcat cggcatccat cagggcatcc agatcgtcag aatcgcaaac taactcaggc
    23281 tccatgcgaa gcagatagct atcaatgcca ctcattcggg ccgcttcgac accatcttgt
    23341 gtggttttac tcccggtgac ttgccagcct cgcgctgaaa gtgacatcgc cagcggcata
    23401 cctaaccacc ctaacccgac aattgcgacc tttttcatcc gttatctcct gatacacctt
    23461 agatctataa ggctacgcta gcgtatcaaa actgacaatt cattctatga atgaatctgt
    23521 tcaataatga taacgacatg ctgcaatgag cagtgaatca tcagtaaccg cgtataccag
    23581 acggtgctcc tctgtaatgc gtcgggacca gaaacctgac aaattatgtt tcaggggttc
    23641 tggtttcccc ttaccttcaa atggtgttct gcgggtatct ttgataagtt cattgatctt
    23701 tttaacaatt cgcttatctg tttcctgcca gtacagataa tcatcccatg attcctcaga
    23761 ccagattagt ttcactcaat aatgtccttt tccgttcctt tgcctgattt caggctatcg
    23821 attgagtcca tcaatctccg ggcgttagcg ggggaacgca gtagataagc cgtctcttcc
    23881 agcgagttgt attcttcgag tgacatcaga acacaagcct ctccattctg acgagtaatg
    23941 aggatcgggg catgatcttc aacggctttc atcattgttg ccgacaaatt ctgacgcgct
    24001 tcgctgtagc taattgtacg catgtaaatc tcctcttttg tacagttcat tgtacaatga
    24061 tgggtgttaa ttaactattt attaattagt ttgtagatca agacattgtc agcgagacga
    24121 aaaatcagac tttgctcttt ttggtgctgt caagttagag gacagtcctc ttagccccct
    24181 tctttccccg ctcattcatt aaacaaatcc atcgccataa aatatataaa aaagcccttg
    24241 ctttctaacg tgaaagtggt ttaggttaaa agacatcagt tgaataaaca ttcacagaga
    24301 cttttatgac acgcgttcaa tttaaacacc accatcatca ccatcatcct gactagtctt
    24361 tcaggcgatg tgtgctggaa gacattcaga tcttccagtg gtgcatgaac gcatgagaaa
    24421 gcccccggaa gatcaccttc cgggggcttt tttattgcgc ggttgttaac ggttcagaca
    24481 ggtttaaaga ggaataagaa aatgacagac aacactcgtt tacgcatagc tatgcagaaa
    24541 tccggccgtt taagtgatga ctcacgcgaa ttactggcgc gctgtggcat taaaatcaat
    24601 cttcacaccc agcgcctgat cgcgatggca gaaaacatgc cgattgatat tctgcgcgtg
    24661 cgtgacgatg acattcccgg tctggtaatg gacggcgtgg tagaccttgg gattatcggc
    24721 gaaaacgtgc tggaagaaga gctgcttaac cgccgcgccc agggtgaaga tccgcgctac
    24781 tttaccttgc gtcgtctgga tttcggcggc tgccgccttt cgctggcaac gccggttgat
    24841 gaagcctggg acggcccgct ctccttaaac ggtaaacgta tcgccacctc ttatcctcac
    24901 ctgctcaagc gttatctcga ccagaaaggt atctctttta aatcctgctt actgaacggt
    24961 tctgtcgaag ttgcgccgcg cgccggactg gcggatgcga tttgcgatct ggtttccacc
    25021 ggtgccacgc tggaagctaa cggcctgcgc gaagtcgaag ttatctaccg ctcgaaagcc
    25081 tgcctgatcc aacgcgatgg cgaaatggaa gaatccaaac agcaactgat cgacaaactg
    25141 ctgacgcgta ttcagggcgt gatccaggcg cgcgaatcaa aatacatcat gatgcacgca
    25201 ccgaccgaac gcctggatga agtcatcgcc ctgctgccag gtgccgaacg cccaaccatt
    25261 ctaccgctgg cgggtgatca acaacgcgta gcgatgcaca tggtcagcag cgaaaccctg
    25321 ttctgggaaa cgatggaaaa actgaaagcg ctgggtgcca gttcaatcct ggtcctgccg
    25381 attgagaaga tgatggagtg atcgccatga gctttaacac aatcattgac tggaatagct
    25441 gtactgcgga gcaacaacgc cagctgttaa tgcgcccggc gatctccgcc tctgaaagca
    25501 ttacccgcac cgttaacgat attctcgata acgtgaaaac gcgtggcgat gaggccctgt
    25561 gtgaatacag cgcgaagttt gataaaacca cggttaccgc gctgaaggtg tctgctgatg
    25621 agatcaccgc cgccagcgaa cgcctgagcg acgagctaaa acaggcgatg gcggtggcag
    25681 taaagaatat tgaaaccttc cacactgcgc aaaaactgcc gccggtagat gtagaaacgc
    25741 agccaggcgt gcgttgccag caggtcacgc gcccgttggc ttcagtgggt ttgtatattc
    25801 ctggcggctc cgccccgctc ttctcaacgg tattaatgct ggcaactccg gcgcgtattg
    25861 cgggctgtaa aaaagtggtg ctgtgctcac cgccgccgat tgccgatgag atcctttatg
    25921 cggcgcagct gtgcggtgtg caggacgtgt ttaacgtcgg cggcgcacag gccattgccg
    25981 cgctggcgtt tggtacggaa tctgtgccga aaatgaacaa aatcttcggg ccgggtaacg
    26041 cctttgtcac cgaagcaaaa cgccaggtaa gccagcgtct ggacggtgcg gcgatcgata
    26101 tgcccgcagg cccgtcggaa gtgctggtga ttactgacag cggcgcaacg ccggatttcg
    26161 tggcttctga tttgctttct caggctgaac acggccctga ctcgcaggta attttactga
    26221 cccccgacgc tgatatggcg cgtcgcgttg ccgaggccgt cgaacgccaa ctggcagaac
    26281 tgccgcgtgc cgaaaccgcc cgccaggcac tgaacgccag ccgcctgatc gtgactaaag
    26341 atttagcgca gtgcgtagag atttccaacc agtacggccc ggaacacctg atcattcaga
    26401 cccgcaacgc ccgcgaactg gtcgatggca tcaccagcgc cggttcggta tttcttggtg
    26461 actggtcacc ggaatcggca ggtgattacg cctccggcac taaccacgtt ctgccgactt
    26521 acggttacac cgccacctgt tccagcctcg ggctggcaga tttccagaag cgcatgaccg
    26581 tgcaggaact gtcgaaagaa ggcttttcca cgctggcttc gaccattgaa acactggccg
    26641 ccgccgagcg cctgaccgcc cacaaaaatg ccgttacttt gcgtgttaac gcccttaagg
    26701 agcaagcatg agcaccgtga ctattaccga tttagcgcgt gaaaacgtcc gcaacctgac
    26761 gccgtatcag tcagcgcgtc gtctgggcgg taacggcgat gtctggctga acgccaacga
    26821 ataccccact gccgtggagt ttcagctcac tcagcaaacg ctcaaccgct atccggaatg
    26881 ccaaccgaaa gtggtgattg aaaattacgc gcaatatgca ggcgtaaaac cggaacaggt
    26941 gctggtcagc cgtggcgcgg acgaaggcat tgaactgctg attcgcgctt tttgcgaacc
    27001 gggtaaagac gccatcctct actgcccgcc aacgtacggc atgtacagcg tcagcgctga
    27061 aaccattggc gtcgagtgcc gcacagtgcc gacgctggac aactggcaac tggacttaca
    27121 gggcatttcc gacaagctgg atggcgtaaa agtggtctat gtttgcagcc ccaacaaccc
    27181 caccgggcaa ctgatcaacc cgcaggattt tcgcaccctg ctggagttaa cccgcggtaa
    27241 ggcgattgtg gttgccgatg aagcctatat cgagttttgc ccgcaggcat cgctggctgg
    27301 ctggctggcg gaatatccgc acctggctat tttgcgcaca ctgtcgaaag cttttgctct
    27361 ggcggggctt cgttgcggat ttacgctggc aaacgaagaa gtcatcaacc tgctgatgaa
    27421 agtgatcgcc ccctacccgc tctcgacgcc ggttgccgac attgccgcac aggcgttaag
    27481 cccgcaggga atcgtcgcca tgcgcgaacg ggtggcgcaa attattacag aacgcgaata
    27541 cctgattgcc gcactgaaag agatcccctg cgtggagcag gttttcgact ctgaaaccaa
    27601 ctacattctg gcgcgcttta aagcctccag tgcggtgttt aaatctttgt gggatcaggg
    27661 cattatctta cgtgatcaga ataaacaacc ctctttaagc ggctgcctgc gaattaccgt
    27721 cggaacccgt gaagaaagcc agtgcgtcat tgacgcctta cgtgcggagc aagtttgatg
    27781 agtcagaagt atctttttat cgatcgcgat ggaaccctga ttagcgaacc gccgagtgat
    27841 tttcaggtgg accgttttga caaactcgcc tttgaacctg gcgtgatccc ggagctgctg
    27901 aggctgcaaa aagcgggcta caagctggtg atgatcacta atcaggatgg tcttggaaca
    27961 caaagtttcc cgcaggcgga tttcgatggc ccgcacaacc tgatgatgca gatcttcacc
    28021 tcgcaaggcg tacagtttga tgaagtgctg atttgcccac acctgcccgc cgatgagtgc
    28081 gactgccgta agccgaaagt aaaactggtg gagcgttatc tcgctgagca agcgatggat
    28141 cgcgccaaca gttatgtgat tggcgatcgc gcgaccgaca ttcaactggc ggaaaacatg
    28201 ggcattaatg gtttacgcta cgaccgcgaa gccctgaact ggccgatgat tggcgaacaa
    28261 ctcaccaaac gagaccgtta cgcccatgta gtgcgcaaca ccaaagagac gcagattgac
    28321 gttcaggtat ggctggatcg cgaaggtggc agcaagatta acaccggcgt tggcttcttt
    28381 gatcacatgc tggatcagat cgctacccac ggtggtttcc gtatggaaat caacgttaaa
    28441 ggcgacctct atatcgacga tcaccacact gtcgaagata ccggtctggc gctgggcgaa
    28501 gcgttaaaaa tagctcttgg cgacaaacgc ggtatttgcc gcttcggttt tgtgctgccg
    28561 atggacgaat gccttgcccg ctgcgcgctg gatatctctg gtcgcccgca cctggaatat
    28621 aaagctgaat ttacctacca gcgcgtgggc gatctcagca ccgagatgat cgaacacttc
    28681 ttccgttcgc tctcatacac catgggcgtg acgctacacc tgaaaaccaa aggtaaaaac
    28741 gatcaccacc gtgtagagag cctgttcaaa gcgtttggtc gcaccctgcg ccaggccatc
    28801 cgcgtggaag gcgacaccct gccctcgtcg aaaggagtgc tgtgatgaac gtggtgatcc
    28861 ttgataccgg ctgcgccaat ttgaactcgg tgaagtctgc cattgcgcgt cacggttatg
    28921 aacccaaagt cagccgtgac ccggacgtag tgttgctggc cgataaactg tttttacccg
    28981 gcgttggcac cgcacaagca gcgatggatc aggtgcgtga gcgcgagcta tttgatctca
    29041 tcaaagcctg tacccaaccg gtgctgggca tctgcttagg gatgcaactc ctgggacgac
    29101 gcagcgaaga gagcaatggc gtcgacttgc tgggcatcat cgacgaagac gtgccgaaaa
    29161 tgaccgactt tggtctgtca ctgccacata tgggctggaa ccgcgtttat ccgcaggcag
    29221 gcaaccgcct gtttcagggg attgaagacg gcgcgtactt ttactttgtt cacagctacg
    29281 caatgccagt aaatccgtgg accatcgccc agtgtaatta cggcgaaccg ttcaccgcgg
    29341 cggtacaaaa agataacttc tacggcgtgc agttccaccc ggagcgttct ggtgccgctg
    29401 gcgctaagtt gctgaaaaac ttcctggaga tgtgatgatt attccggcat tagatttaat
    29461 cgacggcacc gtggtgcgtc tccatcaggg cgattacggc aaacaacgcg attacggtaa
    29521 cgacccgctg ccacgcttac aggattacgc tgcgcaaggt gccaaagtgt tgcacctggt
    29581 ggatctgacc ggggcaaaag atccggctaa acgtcaaatc ccgctgatta aaaccctggt
    29641 cgcgggcgtt aatgttccgg tgcaggttgg cggcggtgtg cgtagtgaag aagacgtagc
    29701 gacgttactg gaagcgggcg ttgcgcgcgt ggtggttggc tccaccgcgg tgaaatcacc
    29761 agaaatggtg aaaggctggt ttgaacgctt cggtgccgat gccttagtgc tggcgctgga
    29821 tgtccgtatt gacgagcaag gtaacaagca ggtggcggtg agcggctggc aagagaactc
    29881 gggcgtttca ctggaacaac tggtggaaac ctatcttccc gtcggcctga aacatgtgct
    29941 atgtaccgat atctcgcgcg acggcacgct ggcgggatct aacgtctctt tatatgaaga
    30001 agtgtgcgcc agatatccac aggtggcatt tcagtcctcc ggcggtattg gcgacattaa
    30061 cgatgtggcg gccctgcgtg gtactggtgt acgcggcgta atagttggtc gtgcattact
    30121 ggaaggtaaa ttcaccgtga aggaggccat cgcatgctgg caaaacgcat aatcccttgt
    30181 ctcgacgttc gtgatggtca ggtggtgaaa ggcgtacagt ttcgcaacca tgaaatcatt
    30241 ggcgatatcg tgccactggc aaaacgctac gccgaagaag gtgcagatga actggtgttc
    30301 tacgatatca ccgcttccag cgatggccgt gtggtagaca aaagctgggt atctcgcgtg
    30361 gcggaagtga tcgacattcc gttttgtgtg gcgggtggga ttaagtctct ggaagatgcc
    30421 gcgaaaattc tttcctttgg cgcggataaa atctccatta actcccctgc gctggcggac
    30481 ccgacgttaa ttactcgcct ggcggatcgc tttggcgtgc agtgtattgt ggtcggcatt
    30541 gatacctggt acgacgccga aaccggtaaa tataatgtga atcaatatac cggcgatgaa
    30601 agccgcaccc gcgtcactca gtgggaaaca ctcgattggg tgcaggaagt gcaaaaacgc
    30661 ggtgccggag aaatcgtcct caacatgatg aatcaggacg gcgtgcgtaa cggctacgac
    30721 ctcgaacagc tgaaaaaagt gcgtgaagtt tgccacgtcc cgctgattgc ctccggtggc
    30781 gcaggcacca tggaacactt tctcgaagcc ttccgcgatg ccgacgttga cggtgcgctg
    30841 gcagcttctg tattccacaa acaaataatc aatattggtg aattaaaagc gtacctggca
    30901 acacagggcg tggagatcag gatatgttaa cagaacaaca acgtcgcgaa ctggactggg
    30961 aaaaaaccga cggtctgatg ccggtgattg tgcaacacgc ggtatctggc gaagtgctaa
    31021 tgctgggcta tatgaacccg gaagccttag acaaaaccat cgaaagcggc aaagtcacct
    31081 tcttctcgcg cactaaacag cgactgtgga ccaaaggcga aacgtcgggt aatttcctca
    31141 acgtagtaaa tattgcgccg gactgcgaca acgacacgtt actggtgctg gcgaatccca
    31201 tcgggccaac ctgccacaaa ggcaccagca gctgcttcgg cgataccgct caccagtggc
    31261 tgttcctgca tcaactggaa caactgctcg ccgaacgtaa atctgccgac ccggaaacct
    31321 cctacaccgc caaactgtat gccagcggca ctaaacgcat tgcgcagaaa gtgggcgaag
    31381 aaggagtgga aaccgcactg gcagcaacgg tacatgaccg ctttgaactg accaacgagg
    31441 cgtctgattt gatgtatcac ctgctggtgt tgttgcagga tcaggatctg gatttaacaa
    31501 cggtgattga gaacctgcgt aaacggcatc agtgagttgc gaggtcagcg gatgcgatgt
    31561 tgttgtcgca tccggagcca ttgcccgatg cgcaagctta tcgtgcctac acattatctc
    31621 aacacacgta ggccggataa gatgcgccag catcgcatcc ggcaaaaaaa acgggcatgg
    31681 tgtcaccacc ctgcccgttt ctcttaaatg cacaataata ttatttcgcg ttgtaattac
    31741 gcagcgcgtt acgcccaagc acaatccccg cgccaaccat gccgcccagc agcactgcca
    31801 gaatcaaggt aattgccttt ttcgggctat cgcgacgaat aggtaacgtc ggtttcatta
    31861 catagcggta agcatgaata tcaagtttat caacgtcaag attgtcgata tccagcaggt
    31921 tttgacgagt ctgatagtag tttgatgaga acaccaacgg acgggtcgct tcgtgcttaa
    31981 tcatcgactc cagcgcttcg ctccccaaaa ggaacatagt atcctgggtt acgtcctgtg
    32041 tctgttgaat ctgcggcttt gtcacctgcg cctgattcgc atactgcaac gcttcctgaa
    32101 tctgacggat acgcagatct ttctgttctt gagcgaccac ttcctgcgtc ttcagagaat
    32161 cctgcaagtt tttcatttgc agcgcgatgt tgtctttcag gtcttgttcc agttcttcat
    32221 ttacctggtc gtcaacttgc tggatatact tcgccagctg tttctgcgca ccttcaggtg
    32281 tttcaccgac ataagaaacc gctaaaggta atgactgatt tttaacggta ggctcaatgg
    32341 tcagtttttc aggttcttcc tgattatcca gcgtttctgc taatgcagaa aatgtcgtgc
    32401 tgaaacgacc aattaacatc gactggatct cagacacttt cggcgctgcc gaaccataaa
    32461 tcacatttat ggcattgtta taaccagcga tttgtcccac atcaggttga gtaacgatag
    32521 ctgttgaagt ccacttttct ttggcaacga ccagataccc tatagccagg gcaatagcca
    32581 caatgacaga aatgatgata gtcatcttgc cacgccatag ttggaccagt aaatcaatca
    32641 aatcaatctg ttccggatca tggttttgcc cagaaacatt attattttct actctcatca
    32701 ttaccctaac ggacgaaata attttaagtc gccatagttt agcgataatc gccatgaatg
    32761 ctataggata aatgataaaa acaatgaatt attacaaagg gaacataaga caatcagggc
    32821 gaaaatcgcc ctgataacaa gatgttagtc actgccaaag agatcgcggg tgtagacttt
    32881 atctgccaca tccttaagct cttctgccat acggttggag ataatgacgt cggcttgttg
    32941 tttgaaggtg gcgagatcac gttccagacg agagttgaag aatgagtctt ctttcatcac
    33001 cggctcgtag atgatcactt caacgccttt cgccttaata cgtttcataa tcccctgaat
    33061 ggaagacgcg cggaagttat ctgaaccgct cttcataatc agacgataaa tacccaccac
    33121 ttgcggcttg cgtgacaaaa tggcatcggc aataaagtct ttacgcgtgc ggtttgcatc
    33181 gacaattgcc gaaatcaggt tattcggcac agactggtag ttcgccagta actgcttggt
    33241 atctttcggc agacaataac caccatagcc aaacgacgga ttgttgtaat ggttgccaat
    33301 acgcggatcg agacaaacac cttcgataat ctggcgagta ttcagaccta aactttctgc
    33361 atagctatcc agttcattaa agtatgctac gcgcatcgcc agataggtat tagcgaaaag
    33421 tttaatcgct tctgcttcag tggagtcggt aaatagtgta gggatatttt gcttaatcgc
    33481 gccttcctgt aataacgccg caaaacgttc tgcgcgttct gaacgctcac cgattacaat
    33541 acgtgacgga tgaaggttat cgtaaagagc tttaccttcg cggaggaatt ccggagagaa
    33601 aataaggtta tcgataccca gacgttcttt aattgatttg gtgaaaccta ccggaattgt
    33661 agatttaatc accattacag cgttgggatt aatttctgtc acatcacgga tgacagcttc
    33721 cacgcttgag gtattaaaat aatttgtttt cgggtcataa tcggtaggcg tggcaataat
    33781 aacgtaatcg gcatttttat acgcgtcata cttatctgtc gtagcacgga aattgagatc
    33841 tttagtcgcc agatactctt caatctcctt atcaacaatc ggtgactgct tcttgttaag
    33901 catgtccact ttggcctgaa cgatatccag tgcaaccact tcgtggtttt gcgcaatcag
    33961 aataccattt gaaagaccaa cataacctgt tcctgaaatt gttattttca ttagctctga
    34021 cttcttccgg tttaacattt agagtggtca ttaatccacc accacatctt catccactgc
    34081 gggattttta acgctgatgt agatgaactg tcaaggcagc gatcctgctg tgcggctctg
    34141 tattatatcg cttttttaat cataaattat aaaaaaaggc cataacctgc cgctttgtat
    34201 aataaaaaag cccggagggt ttctccgggc cttgcattga ttaatcgatt taaatcagat
    34261 taatccagcc attcggtatg gaacacacct tctttatcaa tgcgcttata agtatgcgca
    34321 ccgaaatagt cacgctgtgc ctggatcagg ttcgcaggca gaacagcggc gcggtagctg
    34381 tcataataag caaccgcagc agcaaaggta ggaaccggga taccgttctg tactgcataa
    34441 gcaacgacat cacgcagcgc ctgctgatag tcatcggcga tttgcttgaa gtacggggcc
    34501 agcagcaggt tagcgatctg cggattttcg gcatatgcat cggtgatttt ctgcaggaac
    34561 tgcgcacgga tgatgcagcc agcacggaaa atcttcgcga tttcgccgta gttcagatcc
    34621 cagttgtact cttcagacgc agcacgcagc tgagagaagc cttgggcgta agaaacgatt
    34681 ttgcccagat acagagcacg acgaactttt tcgatgaact cagccttgtc gccagctggc
    34741 tgcgcttgcg ggccagagag aactttagac gcggcaacgc gctgatcttt cagagacgag
    34801 atataacgtg caaacacaga ctcggtaata agcgacagtg gttcaccgag atccagcgcg
    34861 ctctggctgg tccatttacc ggtacctttg ttagccgctt catccagaat cacatcaacc
    34921 aggtagttac cgtcttcatc ttttttggtg aagatatctt tggtgatgtc gatcaggtaa
    34981 ctgctcagtt caccgttatt ccactcggta aaggtctgag ccagttcttc gttggtgagg
    35041 ttcaggccac ctttaagcag agaataggct tcagcaatca gttgcatatc tccgtattca
    35101 ataccattgt gaaccatctt cacataatgg cctgcgccat cggcaccgat ataggtaacg
    35161 catggctcac cgtcttcagc cactgcggcg attttggtca ggatcggcgc aaccagttca
    35221 taggcttctt tctgcccacc aggcataatg gaaggacctt tcagcgcacc ttcttcgcct
    35281 ccagagacac cggtaccaat gaagttaaag ccttcagcag aaagctcacg gttacgacga
    35341 atggtgtcct ggaagaaggt gttaccacca tcaatgatga tgtcgccttt atcgaggtat
    35401 ggtttgagag aatcaatagc agcatccgtg cctgcacctg ctttcaccat taacaggatg
    35461 cgacgaggcg tttccagaga ttcaacaaac tctttcaccg tatagtaagg aaccagtttt
    35521 ttgcctggat tttcggcaat cacttcttcc gttttctcac gggaacggtt gaaaatagag
    35581 acggtataac cacggctttc gatgttgagc gcaaggttgc gtcccatcac tgccataccg
    35641 acgacgccga tctgttgctt tgacattgtt tactcctgtc aggggttacc gcgagatgat
    35701 tgtataaagt tataacgaac tttagaaact
//
LOCUS       DADSYM010000030        35723 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2415
            SAMN08039579-rid11723173.denovo.030, whole genome shotgun sequence.
ACCESSION   DADSYM010000030 DADSYM010000000
VERSION     DADSYM010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039579
            Sequence Read Archive: SRR6362533
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 35723)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35723)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980162.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 03:39:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,484
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 4,347
            CDSs (with protein)               :: 4,347
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 43 of 85
            Pseudo Genes (incomplete)         :: 37 of 85
            Pseudo Genes (internal stop)      :: 19 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35723
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039579-rid11723173.denovo.030"
                     /strain="LGE2415"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8302 N 95.9628 W"
                     /collection_date="2015-08-24"
     gene            complement(94..3243)
                     /locus_tag="J1Y83_001253"
     CDS             complement(94..3243)
                     /locus_tag="J1Y83_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit"
                     /protein_id="HBA7629419.1"
                     /translation="MPNFFIDRPIFAWVIAIIIMLAGGLAILKLPVAQYPTIAPPAVT
                     ISASYPGADAKTVQDTVTQVIEQNMNGIDNLMYMSSNSDSTGTVQITLTFESGTDADI
                     AQVQVQNKLQLAMPLLPQEVQQQGVSVEKSSSSFLMVVGVINTDGTMTQEDISDYVAA
                     NMKDAISRTSGVGDVQLFGSQYAMRIWMNPNELNKFQLTPVDVITAIKAQNAQVAAGQ
                     LGGTPPVKGQQLNASIIAQTRLTSTEEFGKILLKVNQDGSRVLLRDVAKIELGGENYD
                     IIAEFNGQPASGLGIKLATGANALDTAAAIRAELAKMEPFFPSGLKIVYPYDTTPFVK
                     ISIHEVVKTLVEAIILVFLVMYLFLQNFRATLIPTIAVPVVLLGTFAVLAAFGFSINT
                     LTMFGMVLAIGLLVDDAIVVVENVERVMAEEGLPPKEATRKSMGQIQGALVGIAMVLS
                     AVFVPMAFFGGSTGAIYRQFSITIVSAMALSVLVALILTPALCATMLKPIAKGDHGEG
                     KKGFFGWFNRMFEKSTHHYTDSVGGILRSTGRYLVLYLIIVVGMAYLFVRLPSSFLPD
                     EDQGVFMTMVQLPAGATQERTQKVLNEVTNYYLTKEKNNVESVFAVNGFGFAGRGQNT
                     GIAFVSLKDWADRPGEENKVEAITMRATRAFSQIKDAMVFAFNLPAIVELGTATGFDF
                     ELIDQAGLGHEKLTQARNQLLAEAAKHPDMLTSVRPNGLEDTPQFKIDIDQEKAQALG
                     VSINDINTTLGAAWGGSYVNDFIDRGRVKKVYVMSEAKYRMLPDDIGDWYVRAADGQM
                     VPFSAFSSSRWEYGSPRLERYNGLPSMEILGQAAPGKSTGEAMELMEQLASKLPTGVG
                     YDWTGMSYQERLSGNQAPSLYAISLIVVFLCLAALYESWSIPFSVMLVVPLGVIGALL
                     AATFRGLTNDVYFQVGLLTTIGLSAKNAILIVEFAKDLMDKEGKGLIEATLDAVRMRL
                     RPILMTSLAFILGVMPLVISTGAGSGAQNAVGTGVMGGMVTATVLAIFFVPVFFVVVR
                     RRFSRKNEDIEHSHTVDHH"
     gene            complement(3266..4459)
                     /gene="acrA"
                     /locus_tag="J1Y83_001254"
     CDS             complement(3266..4459)
                     /gene="acrA"
                     /locus_tag="J1Y83_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter periplasmic
                     adaptor subunit AcrA"
                     /protein_id="HBA7629420.1"
                     /translation="MNKNRGFTPLAVVLMLSGSLALTGCDDKQAQQGGQQMPAVGVVT
                     VKTEPLQITTELPGRTSAYRIAEVRPQVSGIILKRNFKEGSDIEAGVSLYQIDPATYQ
                     AAYDSAKGDLAKAQAAANIAQLTVNRYQKLLGTQYISKQEYDQALADAQQANAAVTAA
                     KAAVETSRINLAYTKVTSPISGRIGKSNVTEGALVQNGQATALATVQQLDPIYVDVTQ
                     SSNDFLRLKQELANGTLKQENGKAKVSLITSDGIKFPQDGTLEFSDVTVDQTTGSITL
                     RAIFPNPDHTLLPGMFVRARLEEGLNPNAILVPQQGVTRTPRGDATVLVVGADDKVET
                     RPIVASQAIGDKWLVTEGLKAGDRVVISGLQKVRPGVQVKAQEVTADNNQQAASGAQP
                     EQSKS"
     gene            4601..5248
                     /gene="acrR"
                     /locus_tag="J1Y83_001255"
     CDS             4601..5248
                     /gene="acrR"
                     /locus_tag="J1Y83_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter transcriptional
                     repressor AcrR"
                     /protein_id="HBA7629421.1"
                     /translation="MARKTKQEAQETRQHILDVALRLFSQQGVSSTSLGEIAKAAGVT
                     RGAIYWHFKDKSDLFSEIWELSESNIGELELEYQAKFPGDPLSVLREILIHVLESTVT
                     EERRRLLMEIIFHKCEFVGEMAVVQQAQRNLCLESYDRIEQTLKHCIEAKMLPADLMT
                     RRAAIIMRGYISGLMENWLFAPQSFDLKKEARDYVAILLEMYLLCPTLRNPATNE"
     gene            5376..8738
                     /locus_tag="J1Y83_001256"
     CDS             5376..8738
                     /locus_tag="J1Y83_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lymphostatin Efa1/LifA"
                     /protein_id="HBA7629422.1"
                     /translation="MTMFQYYKRSRHFVFSAFIAFVFVLLCQNTAFARASSNGDLPTK
                     ADLQAQLDSLNKQKDLSAQDKLVQQDLTDTLATLDKIDRVKEETVQLRQKVAEAPEKM
                     RQATAALTALSDVDNDEETRKILSTLSLRQLETRVAQALDDLQNAQNDLASYNSQLVS
                     LQTQPERVQNAMYNASQQLQQIRSRLDGTDVGETALRPSQKVLMQVQQALLNAEIDQQ
                     RKSLEGNTVLQDTLQKQRDYVTANSARLEHQLQLLQEAVNSKRLTLTEKTAQEAVSPD
                     EAARIQANPLVKQELEINQQLSQRLITATQNGNQLMQQNIKVKNWLERALQSERNIKE
                     QIAVLKGSLLLSRILYQQQQTLPSADELENMTNRIADLRLEQFEVNQQRDALFQSDAF
                     VSKLEEGHTNEVNSEVHDALLQVVDMRRELLDQLNKQLGNQLMMAINLQINQQQLMSV
                     SKNLKSILTQQIFWVNSNRPMDWDWIKAFPQTLKDEFKSMKITVNWEKAWPAVFIAFL
                     AGLPLLLIAGLIHWRLGWLKAYQQKLASAVGSLRNDSQLNTPKAILIDLIRALPVCLI
                     ILAVGLILLTMQLNISELLWSFSKKLAIFWLVFGLCWKVLEKNGVAVRHFGMPEQQTS
                     HWRRQIVRISLALLPIHFWSVVAELSPLHLMDDVLGQAMIFFNLLLIAFLVWPMCRES
                     WRDKESHTMRLVTITVLSIIPIALMVLTATGYFYTTLRLSGRWIETVYLVIIWNLLYQ
                     TVLRGLSVAARRIAWRRALARRQNLVKEGAEGAEPPEEPTIALEQVNQQTLRITMLLM
                     FALFGVMFWAIWSDLITVFSYLDSITLWHYNATEAGAAVVKNVTMGSLLFAIIASMVA
                     WALIRNLPGLLEVLVLSRLNMRQGASYAITTILNYIIIAVGAMTVFGSLGVSWDKLQW
                     LAAALSVGLGFGLQEIFGNFVSGLIILFERPVRIGDTVTIGSFSGTVSKIRIRATTIT
                     DFDRKEVIIPNKAFVTERLINWSLTDTTTRLVIRLGVAYGSDLEKVRKVLLKAATEHP
                     RVMHEPMPEVFFTAFGASTLDHELRLYVRELRDRSRTVDELNRTIDQLCRENDINIAF
                     NQLEVHLHNEKGDEVTEVKRDYKGDDPTPAVG"
     gene            complement(8950..9111)
                     /gene="rsmS"
                     /locus_tag="J1Y83_001257"
     CDS             complement(8950..9111)
                     /gene="rsmS"
                     /locus_tag="J1Y83_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pleiotropic regulatory protein RsmS"
                     /protein_id="HBA7629423.1"
                     /translation="MSLENAPDDVKLAVDLIVLLEENQIPARTVLRALDIVKRDYEKK
                     LTRDDEAEK"
     gene            complement(9125..9652)
                     /gene="priC"
                     /locus_tag="J1Y83_001258"
     CDS             complement(9125..9652)
                     /gene="priC"
                     /locus_tag="J1Y83_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N''"
                     /protein_id="HBA7629424.1"
                     /translation="MKTTLLLEKLEGQLATLRQRCAPVAQFATLSARFDRHLFQTRAT
                     TLQACLDEAGDNLAALRHAVEQQQLPQVAWLAEHLAAQLEAIAREATAWSLREWDSAP
                     PKIARWQRKRIQHQDFERRLREMVAERRARLARATDLVEQQTLHREVEAYEARLARCR
                     HALEKIENRLARLTR"
     gene            9722..10099
                     /locus_tag="J1Y83_001259"
     CDS             9722..10099
                     /locus_tag="J1Y83_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF454 family protein"
                     /protein_id="HBA7629425.1"
                     /translation="MQRIILIIIGWLAVVLGTLGVVLPVLPTTPFILLAAWCFARSSP
                     RFHAWLLYRSWFGSYLRFWQKHHAMPRGAKPRAILLILITFTISLWFVQMPWVRIMLL
                     VILACLLFYMWRIPVIDEKQEKH"
     gene            10252..10803
                     /gene="apt"
                     /locus_tag="J1Y83_001260"
     CDS             10252..10803
                     /gene="apt"
                     /locus_tag="J1Y83_001260"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HBA7629426.1"
                     /translation="MTATAQQLEYLKNSIKSIQDYPKPGILFRDVTSLLEDPKAYALS
                     IDLLVERYKNAGITKVVGTEARGFLFGAPVALGLGVGFVPVRKPGKLPRETISETYDL
                     EYGTDQLEIHVDAIKPGDKVLVVDDLLATGGTIEATVKLIRRLGGEVADAAFIINLFD
                     LGGEQRLEKQGITSYSLVPFPGH"
     gene            10932..12863
                     /gene="dnaX"
                     /locus_tag="J1Y83_001261"
     CDS             10932..12863
                     /gene="dnaX"
                     /locus_tag="J1Y83_001261"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HBA7629427.1"
                     /translation="MSYQVLARKWRPQTFADVVGQEHVLTALANGLSLGRIHHAYLFS
                     GTRGVGKTSIARLLAKGLNCETGITATPCGVCDNCREIEQGRFVDLIEIDAASRTKVE
                     DTRDLLDNVQYAPARGRFKVYLIDEVHMLSRHSFNALLKTLEEPPEHVKFLLATTDPQ
                     KLPVTILSRCLQFHLKALDVEQIRHQLEHILNEEHIAHEPRALQLLARAAEGSLRDAL
                     SLTDQAIASGDGQVSTQAVSAMLGTLDDDQALSLVEAMVEANGERVMALINEAAARGI
                     EWEALLVEMLGLLHRIAMVQLSPAALGNDMAAIELRMRELARTIPPTDIQLYYQTLLI
                     GRKELPYAPDRRMGVEMTLLRALAFHPRMPLPEPEVPRQSFAPVAQTAVMTPTQVPPQ
                     PQSAPQQAPTVPLPETTSQVLAARQQLLRVQGATKAKKSEPAAATRARPVNNAALERL
                     ASVTDRVQARPVPSALEKAPAKKEAYRWKATTPVMQQKEVVATPKALKKALEHEKTPE
                     LAAKLAAEAIERDPWAAQVSQLSLPKLVEQVALNAWKEESDNAVCLHLRSSQRHLNNR
                     GAQQKLAEALSTLKGSTVELTIVEDDNPAVRTPLEWRQAIYEEKLAQARESIIADNNI
                     QTLRRFFDAELDEESIRPI"
     gene            12916..13245
                     /locus_tag="J1Y83_001262"
     CDS             12916..13245
                     /locus_tag="J1Y83_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001364632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HBA7629428.1"
                     /translation="MFGKGGLGNLMKQAQQMQEKMQKMQEEIAQLEVTGESGAGLVKV
                     TINGAHNCRRVEIDPSLLEDDKEMLEDLVAAAFNDAARRIEETQKEKMASVSSGMQLP
                     PGFKMPF"
     gene            13245..13850
                     /gene="recR"
                     /locus_tag="J1Y83_001263"
     CDS             13245..13850
                     /gene="recR"
                     /locus_tag="J1Y83_001263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecR"
                     /protein_id="HBA7629429.1"
                     /translation="MQTSPLLTQLMEALRCLPGVGPKSAQRMAFTLLQRDRSGGMRLA
                     QALTRAMSEIGHCADCRTFTEQEVCNICSNPRRQENGQICVVESPADIYAIEQTGQFS
                     GRYFVLMGHLSPLDGIGPDDIGLDRLEQRLAEEKITEVILATNPTVEGEATANYIAEL
                     CAQYDVEASRIAHGVPVGGELEMVDGTTLSHSLAGRHKIRF"
     gene            13960..15834
                     /gene="htpG"
                     /locus_tag="J1Y83_001264"
     CDS             13960..15834
                     /gene="htpG"
                     /locus_tag="J1Y83_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003903109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HBA7629430.1"
                     /translation="MKGQETRGFQSEVKQLLHLMIHSLYSNKEIFLRELISNASDAAD
                     KLRFRALSNPDLYEGDGELRVRVSFDKDKRTLTISDNGVGMTRDEVIDHLGTIAKSGT
                     KSFLESLGSDQAKDSQLIGQFGVGFYSAFIVADKVTVRTRAAGEKPENGVFWESAGEG
                     EYTVADITKEDRGTEITLHLREGEDEFLDDWRVRSIISKYSDHIALPVEIEKREEKDG
                     ETVISWEKINKAQALWTRNKSEITDEEYKEFYKHIAHDFNDPLTWSHNRVEGKQEYTS
                     LLYIPSQAPWDMWNRDHKHGLKLYVQRVFIMDDAEQFMPNYLRFVRGLIDSSDLPLNV
                     SREILQDSTVTRNLRNALTKRVLQMLEKLAKDDAEKYQTFWQQFGLVLKEGPAEDFAN
                     QEAIAKLLRFASTHTDSSAQTVSLEDYVSRMKEGQEKIYYITADSYAAAKSSPHLELL
                     RKKGIEVLLLSDRIDEWMMNYLTEFDGKPFQSVSKVDESLEKLADEVDESAKEAEKAL
                     TPFIDRVKALLGERVKDVRLTHRLTDTPAIVSTDADEMSTQMAKLFAAAGQKVPEVKY
                     IFELNPDHVLVKRAADTEDEAKFSEWVELLLDQALLAERGTLEDPNLFIRRMNQLLVS
                     "
     gene            16015..16659
                     /gene="adk"
                     /locus_tag="J1Y83_001265"
     CDS             16015..16659
                     /gene="adk"
                     /locus_tag="J1Y83_001265"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HBA7629431.1"
                     /translation="MRIILLGAPGAGKGTQAQFIMEKYGIPQISTGDMLRAAVKSGSE
                     LGKQAKDIMDAGKLVTDELVIALVKERIAQEDCRNGFLLDGFPRTIPQADAMKEAGIN
                     VDYVLEFDVPDELIVDRIVGRRVHAPSGRVYHVKFNPPKVEGKDDVTGEELTTRKDDQ
                     EETVRKRLVEYHQMTAPLIGYYSKEAEAGNTKYAKVDGTKPVAEVRAALEKILG"
     gene            16791..17753
                     /gene="hemH"
                     /locus_tag="J1Y83_001266"
     CDS             16791..17753
                     /gene="hemH"
                     /locus_tag="J1Y83_001266"
                     /EC_number="4.99.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HBA7629432.1"
                     /translation="MRQTKTGILLANLGTPDAPTPEAVKRYLKQFLSDRRVVDTSRLL
                     WWPLLRGVILPLRSPRVAKLYASVWMEGGSPLMVYSRQQQQALAQRLPETPVALGMSY
                     GSPSLESAVDELLAEHVDHIVVLPLYPQYSCSTVGAVWDELARILARKRSIPGISFIR
                     DYADNHDYINALANSVRASFAKHGEPDLLLLSYHGIPQRYADEGDDYPQRCRTTTREL
                     ASALEMAPEKVMMTFQSRFGREPWLMPYTDETLKMLGEKGVGHIQVMCPGFAADCLET
                     LEEIAEQNREVFLGAGGKKYEYIPALNATPEHIEMMANLVAAYR"
     gene            complement(17728..18696)
                     /gene="aes"
                     /locus_tag="J1Y83_001267"
     CDS             complement(17728..18696)
                     /gene="aes"
                     /locus_tag="J1Y83_001267"
                     /EC_number="3.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000846119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl esterase"
                     /protein_id="HBA7629433.1"
                     /translation="MKPENKLPVLDLISAEMKTVVNTLQPDLPPWPATGTIAEQRQYY
                     TLERRFWNVGAPEMATRAYRVPTKYGQVKTRIFYPQPDSPATLFYLHGGGFILGNLDT
                     HDRIMRLLASYSQCTVIGIDYTLSPEARFPQAIEEIVAACCYFHQQAEDYQINMSRIG
                     FAGDSAGAMLALASALWLRDKQIDCGKVAGVLLWYGLYGLRDSVTRRLLGGVWDGLTQ
                     QDLQMYEEAYLSNDADRESPYYCLFNNDLTREVPPCFIAGAEFDPLLDDSRLLYQTLA
                     AHQQPCEFKLYPGTLHAFLHYSRMMKTADEALRDGARFFSDRLQQD"
     gene            18848..20152
                     /locus_tag="J1Y83_001268"
     CDS             18848..20152
                     /locus_tag="J1Y83_001268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015965517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inosine/guanosine kinase"
                     /protein_id="HBA7629434.1"
                     /translation="MKFPGKRKSKHYFPVNARDPLLQQFQPENETSAAWVVGIDQTLV
                     DIEAKVDDEFIERYGLSAGHSLVIEDDVAEALYQELKQKNLITHQFAGGTIGNTMHNY
                     SVLADDRSVLLGVMCSNIEIGSYAYRYLCNTSSRTDLNYLQGVDGPIGRCFTLIGESG
                     ERTFAISPGHMNQLRAESIPEDVIAGASALVLTSYLVRCKPGEPMPEATMKAIEYAKK
                     YNVPVVLTLGTKFVIAENPQWWQQFLKDHVSILAMNEDEAEALTGESDPLLASDKALD
                     WVDLVLCTAGPIGLYMAGFTEDEAKRKTQHPLLPGAIAEFNQYEFSRAMRHKDCQNPL
                     RVYSHIAPYMGGPEKIMNTNGAGDGALAALLHDITANSYHRSNVPNSSKHKFTWLTYS
                     SLAQVCKYANRVSYQVLNQHSPRLTRGLPEREDSLEESYWDR"
     gene            complement(20282..21958)
                     /locus_tag="J1Y83_001269"
     CDS             complement(20282..21958)
                     /locus_tag="J1Y83_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001582195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Kef family K(+) transporter"
                     /protein_id="HBA7629435.1"
                     /translation="MHHATPLITTIVGGLVLAFILGMLANKLRISPLVGYLLAGVLAG
                     PFTPGFVADTKLAPELAELGVILLMFGVGLHFSLKDLMAVKSIAIPGAIAQIAVATLL
                     GMALSAVLGWSLMTGIVFGLCLSTASTVVLLRALEERQLIDSQRGQIAIGWLIVEDLV
                     MVLTLVLLPAVAGMMEQGDVGFATLAVDMGITIGKVIAFIAIMMLVGRRLVPWIMARS
                     AATGSRELFTLSVLALALGIAFGAVELFDVSFALGAFFAGMVLNESELSHRAAHDTLP
                     LRDAFAVLFFVSVGMLFDPLILIQQPLAVLATLAIILFGKSVAALFLVRLFGHSQRTA
                     LTIAASLAQIGEFAFILAGLGMALNLLPQAGQNLVLAGAILSIMLNPVLFALLEKYLA
                     KTETLEEQTLEEAIEEEKQIPVDICNHALLVGYGRVGSLLGEKLLASDIPLVVIETSR
                     TRVDELRERGVRAVLGNAANEEIMQLAHLECAKWLILTIPNGYEAGEIVASARAKNPD
                     IEIIARAHYDDEVAYITERGANQVVMGEREIARTMLELLETPPAGEVVTR"
     gene            complement(22197..23417)
                     /locus_tag="J1Y83_001270"
     CDS             complement(22197..23417)
                     /locus_tag="J1Y83_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBA7629436.1"
                     /translation="MAMSEQTQPVAGAAASTTKARTSFGILGAISLSHLLNDMIQSLI
                     LAIYPLLQSEFSLTFMQIGMITLTFQLASSLLQPVVGYWTDKYPMPWSLPIGMCFTLS
                     GLVLLALAGSFGAVLLAAALVGTGSSVFHPESSRVARMASGGRHGLAQSIFQVGGNFG
                     SSLGPLLAAVIIAPYGKGNVAWFVLAALLAIVVLAQISRWYSAQHRMNKGKPKATIIN
                     PLPRNKVVLAVSILLILIFSKYFYMASISSYYTFYLMQKFGLSIQNAQLHLFAFLFAV
                     AAGTVIGGPVGDKIGRKYVIWGSILGVAPFTLILPYASLHWTGVLTVIIGFILASAFS
                     AILVYAQELLPGRIGMVSGLFFGFAFGMGGLGAAVLGLIADHTSIELVYKICAFLPLL
                     GMLTIFLPDNRHKD"
     gene            23635..25287
                     /locus_tag="J1Y83_001271"
     CDS             23635..25287
                     /locus_tag="J1Y83_001271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-sugar
                     hydrolase/5'-nucleotidase"
                     /protein_id="HBA7629437.1"
                     /translation="MKLLQRGVALALLTTFTLASETALAYEQDKTYKITVLHTNDHHG
                     HFWRNEYGEYGLAAQKTLVDGIRKEVAAEGGSVLLLSGGDINTGVPESDLQDAEPDFR
                     GMNLVGYDAMAIGNHEFDNPLTVLRQQEKWAKFPLLSANIYQKSTGERLFKPWALFKR
                     QDLKIAVIGLTTDDTAKIGNPEYFTDIEFRKPADEAKLVIQELQQTEKPDIIIAATHM
                     GHYDNGEHGSNAPGDVEMARALPAGSLAMIVGGHSQDPVCMAAENKKQVDYVPGTPCK
                     PDQQNGIWIVQAHEWGKYVGRADFEFRNGEMKMVNYQLIPVNLKKKVTWEDGKSERVL
                     YTPEIAENQQMISLLSPFQNKGKAQLEVKIGETNGRLEGDRDKVRFVQTNMGRLILAA
                     QMDRTGADFAVMSGGGIRDSIEAGDISYKNVLKVQPFGNVVVYADMTGKEVIDYLTAV
                     AQMKPDSGAYPQFANVSFVAKDGKLNDLKIKGEPVDPAKTYRMATLNFNATGGDGYPR
                     LDNKPGYVNTGFIDAEVLKAYIQKSSPLDVSVYEPKGEVSWQ"
     gene            complement(25324..25803)
                     /gene="ybaK"
                     /locus_tag="J1Y83_001272"
     CDS             complement(25324..25803)
                     /gene="ybaK"
                     /locus_tag="J1Y83_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro)/Cys-tRNA(Cys) deacylase YbaK"
                     /protein_id="HBA7629438.1"
                     /translation="MTPAVKLLEKNKISFQIHTYEHDPAETNFGDEVVKKLGLNPDQV
                     YKTLLVAVNGDMKHLAVAVTPVAGQLDLKKVAKALGAKKVEMADPMVAQRSTGYLVGG
                     ISPLGQKKRLPTIIDAPAQEFATIYVSGGKRGLDIELAASDLAKILDAKFADIARRD"
     gene            complement(26007..26801)
                     /locus_tag="J1Y83_001273"
     CDS             complement(26007..26801)
                     /locus_tag="J1Y83_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA7629439.1"
                     /translation="MDLLYRVKTLWAALRGNHYTWPAIDISLPGNRHFHLIGSIHMGS
                     HDMAPLPTRLLKKLKNADALIVEADVSTSDTPFANLPACEALEERISEEQLQNLQHIS
                     QKMGISPSLFSTQPLWQIAMVLQATQAQKLGLRAEYGIDYQLLQAAKQQHKPVIELEG
                     AENQIAMLLQLPDKGLALLDDTLTHWHTNARLLQQMMSWWLNTPPQNNEITLPNTFSQ
                     SLYDVLMHQRNLAWRDKLRAMPPGRYVVAVGALHLYGEGNLPQMLR"
     gene            26934..27236
                     /locus_tag="J1Y83_001274"
     CDS             26934..27236
                     /locus_tag="J1Y83_001274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000057540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HBA7629440.1"
                     /translation="MAQKKNIRSFRDAWLADFFVHSTPHRKIPAEIHTTLSRKLDIIN
                     AATSHRDLRSPPGNRYEELSGKLQEYSSIRVNKQYRLIFKWVNGKAEELFLDPHNY"
     gene            27272..27613
                     /locus_tag="J1Y83_001275"
     CDS             27272..27613
                     /locus_tag="J1Y83_001275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antidote protein"
                     /protein_id="HBA7629441.1"
                     /translation="MKQATRKPTTPGDILLYEYLEPLDLKINELAELLHVHRNSVSAL
                     INNNRKLTTEMAFRLAKVFDTTVDFWLNLQAAVDLWEVENNMRTQEELGRIETVAEYL
                     ARREERAKKVA"
     gene            complement(27671..30175)
                     /gene="copA"
                     /locus_tag="J1Y83_001276"
     CDS             complement(27671..30175)
                     /gene="copA"
                     /locus_tag="J1Y83_001276"
                     /EC_number="7.2.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-exporting P-type ATPase CopA"
                     /protein_id="HBA7629442.1"
                     /translation="MSQTIDLTLDGLSCGHCVKRVKESLEQRPDVEQADVSITEAHVT
                     GTASAEQLIETIKQAGYDASVSHPKAKPLAESSIPSEALTAVSEALPAATADDDDSQQ
                     LLLSGMSCASCVTRVQNALQSVPGVTQARVNLAERTALVMGSASPQDLVQAVEKAGYG
                     AEAIEDDAKRRERQQETAVATMKRFRWQAIVALAVGIPVMVWGMIGDNMMVTADNRSL
                     WLVIGLITLAVMVFAGGHFYRSAWKSLLNGAATMDTLVALGTGVAWLYSMSVNLWPQW
                     FPMEARHLYYEASAMIIGLINLGHMLEARARQRSSKALEKLLDLTPPTARLVTDEGEK
                     SVPLAEVQPGMLLRLTTGDRVPVDGEITQGEAWLDEAMLTGEPIPQQKGEGDSVHAGT
                     VVQDGSVLFRASAVGSHTTLSRIIRMVRQAQSSKPEIGQLADKISAVFVPVVVVIALI
                     SAAIWYFFGPAPQIVYTLVIATTVLIIACPCALGLATPMSIISGVGRAAEFGVLVRDA
                     DALQRASTLDTVVFDKTGTLTEGKPQVVAVKTFADIDEAQALRLAAALEQGSSHPLAR
                     AILDKAGDMQLPQVNGFRTLRGLGVSGEAEGHALLLGNQALLNDQQVDTKAIEADISA
                     QASQGATPVLLAVDGKAVALLAVRDPLRSDSVAALQRLHKAGYRLVMLTGDNPTTANA
                     IAKEAGIDEVIAGVLPDGKAEAIKRLQSEGRQVAMVGDGINDAPALAQADVGIAMGGG
                     SDVAIETAAITLMRHSLMGVADALAISRATLRNMKQNLLGAFIYNSIGIPVAAGILWP
                     FTGTLLNPVVAGAAMALSSITVVSNANRLLRFKPKE"
     gene            30437..31369
                     /gene="glsA"
                     /locus_tag="J1Y83_001277"
     CDS             30437..31369
                     /gene="glsA"
                     /locus_tag="J1Y83_001277"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HBA7629443.1"
                     /translation="MLDANKLQQAVDQAYTQFHSLNGGQNADYIPFLANVPGQLAAVS
                     IVTSDGNVYSAGDSDYRFALESISKVCTLALALEDVGPQAVQDKVGADPTGLPFSSVI
                     ALELHGGKPLSPLVNAGAIATTSLINAENTEQRWQRILHIQQQLAGEQVALSDEVNQS
                     EQTTNFHNRAIAWLLYSAGYLYCDAMEACDVYTRQCSTLINTIELATLGATLAAGGVN
                     PLTHKRVLQADNVPYILAEMMMEGLYGRSGDWAYRVGLPGKSGVGGGILAVVPGVMGI
                     AAFSPPLDEEGNSVRGQKMVASVAKQLGYNVFKG"
     gene            31372..32664
                     /locus_tag="J1Y83_001278"
     CDS             31372..32664
                     /locus_tag="J1Y83_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBA7629444.1"
                     /translation="MMNTEGNNGNKPLGLWNVVSIGIGAMVGAGIFALLGQAALLMEA
                     STWVAFAFGGIVAMFSGYAYARLGASYPSNGGIIDFFRRGLGNGVFSLALSLLYLLTL
                     AVSIAMVARAFGAYAVQFLHEGSQEEHLILLYALGIIAVMTLFNSLSNHAVGRLEVIL
                     VGIKMMILLLLIIAGVWSLQPAHISVSAPPSSGAFFSCIGITFLAYAGFGMMANAADK
                     VKDPQVIMPRAFLVAIGVTTLLYISLALVLLSDVSALELEKYADTAVAQAASPLLGHV
                     GYVIVVIGALLATASAINANLFAVFNIMDNMGSERELPKLMNKPLWRQSTWGNIIVVV
                     LIMLMTAALNLGSLASVASATFLICYLAVFVVAIRLRHDIHASLPILIVGTLVMLLVI
                     VGFIYSLWSQGSRALIWIIGALLLSLIVAMVMKRNKTV"
     gene            32789..33196
                     /locus_tag="J1Y83_001279"
     CDS             32789..33196
                     /locus_tag="J1Y83_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator CueR"
                     /protein_id="HBA7629445.1"
                     /translation="MNISDVAKITGLTSKAIRFYEEKGLVTPPMRSENGYRTYTQQHL
                     NELTLLRQARQVGFNLEESGELVNLFNDPQRHSADVKRRTLEKVAEIEQHIEELQSMR
                     NQLLALANACPGDDSADCPIIENLSGCCHHRAG"
     gene            complement(33197..33933)
                     /locus_tag="J1Y83_001280"
                     /pseudo
     CDS             complement(33197..33933)
                     /locus_tag="J1Y83_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001589011.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(33997..34203)
                     /locus_tag="J1Y83_001281"
     CDS             complement(33997..34203)
                     /locus_tag="J1Y83_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000664217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA7629446.1"
                     /translation="MKEQRKIPLTHIMIIGAFIFAFLQVVLLASLVHAVNVNNEIQEG
                     LFQSGRIMVESLQHILSVQTGING"
     gene            complement(34200..34622)
                     /locus_tag="J1Y83_001282"
     CDS             complement(34200..34622)
                     /locus_tag="J1Y83_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA7629447.1"
                     /translation="MMNSSIKSFSLLAVILLAGCSSPASRIADCQAQGVSHDTCYLAE
                     QQRQTAILSASEAQAFKNAEAAQHAQAAKKAIYKGFGMTFRMSSKNFAYLNDSLCAID
                     EDNADATVYQSGLYNVIVYHHTGKVALMKEGQFVGYLK"
     gene            complement(34708..35604)
                     /locus_tag="J1Y83_001283"
     CDS             complement(34708..35604)
                     /locus_tag="J1Y83_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001409678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adhesin"
                     /protein_id="HBA7629448.1"
                     /translation="MSQSEIEQKITRFLERTDNSPAAYTYLTEHHYIPSETPDTTQTP
                     PVQTDPDAGQKTVAATGDAQTPARYQSMINARQSAVTDAQQTQITEQQAQIVATQKTL
                     AATGDTQNTAHYQEMINARLAAQNEANQRTTTEQGQKMNALTTDVAAQQQKERAQYDK
                     QMQSLAQKSVQAHEQIESLRQDSAQTQQQLSNTQKRVADNSQQINTLNNHFSSLKNEV
                     EDNRKEANAGTASAIAIASQPQVKTDDLMMVSAGAGTFNGESAVSVGTSFNAGTHTVL
                     KAGISADTQSDFGAGVGVGYSF"
BASE COUNT         8676 a         9317 c         9348 g         8382 t
ORIGIN      
        1 tgagatcctg agttggtggt tcaatactcc ttaatgttcg taggttatgc ataaaaaagg
       61 ccgcttgcgc ggccttagtg attacacgtt gtatcaatga tgatcgacag tatggctgtg
      121 ctcgatatct tcattcttgc ggctaaagcg gcggcgaacc accacaaaga ataccggaac
      181 gaagaagatt gccagtaccg ttgctgtcac catcccgccc attacaccgg tacctactgc
      241 gttctgtgcg ccggaaccag caccagtact gataaccagc ggcataacgc cgaggataaa
      301 cgccaacgag gtcatcagaa ttgggcgtaa acgcatccgc accgcatcaa gcgtcgcttc
      361 aatcagacct ttaccttctt tatccatcaa gtctttggcg aattcgacga taagtatcgc
      421 gttcttcgcc gacaaaccaa tggttgtgag caggcctacc tggaagtaaa cgtcattggt
      481 caggccacgg aaggtggcag ccagcaacgc accgataacc cccagcggaa cgaccagcat
      541 aacggagaac ggaatcgacc agctctcgta cagcgccgcc agacacagga acacgacaat
      601 caacgaaatc gcgtacagtg aaggtgcctg gttgccggag agacgttcct gataggacat
      661 tcccgtccag tcatagccaa caccggtagg cagtttgctc gccagttgtt ccatcagctc
      721 cattgcttca ccggtacttt tacccggtgc cgcctggcct aagatttcca tagatggcag
      781 gccgttgtaa cgttccagac gcggcgaacc gtactcccaa cgagaagagg agaacgccga
      841 gaacggcacc atctgaccat cagcagcacg aacataccag tcgccgatat catccggcag
      901 catacggtat ttcgcttctg acatgacgta aactttcttc acacgaccgc ggtcgataaa
      961 gtcgttcaca tagctaccgc cccatgcagc gcccagagtg gtgttaatgt cgttgataga
     1021 aacacccagc gcctgcgctt tttcctggtc gatatcaatc ttaaactgcg gggtatcttc
     1081 cagaccgttt ggacgtacgc tggtcaacat atcagggtgc ttcgctgctt ctgcaagcaa
     1141 ctggttacgc gcctgagtca gtttttcgtg accaaggcca gcctggtcaa tcagctcaaa
     1201 gtcaaagccg gttgcggtac ccagttccac gattgcgggc aggttaaagg cgaaaaccat
     1261 cgcatctttg atttgcgaga aagcacgtgt tgcacgcatg gtaatcgctt caactttgtt
     1321 ttcttcgccc ggacgatcgg cccagtcctt caaggaaacg aacgcaatac cggtattctg
     1381 accacgtccc gcaaagccga agccgttaac ggcgaacacc gactcaacgt tgttcttttc
     1441 tttggtcaga tagtaattcg ttacctcatt gagcactttc tgcgtacgtt cctgcgttgc
     1501 accagctggc agctgaacca tggtcataaa tacgccctgg tcctcatctg gcaagaagga
     1561 gcttggcaga cgcacgaaca gataggccat accgaccacg atgatcagat acagcaccag
     1621 gtaacgcccc gtactgcgca gaataccgcc tacgctgtcg gtgtagtggt gcgtgctctt
     1681 ctcgaacatg cggttaaacc agccgaagaa gcctttttta ccttccccgt gatcgccttt
     1741 ggcaatcggt ttcagcatgg tggcacaaag agccggagtc aggatcaacg ccaccagtac
     1801 cgacagcgcc attgctgaaa caatggtaat agagaactga cgatagatag caccagtaga
     1861 accgccaaag aaggccatcg gtacgaatac cgccgacagt accatcgcga taccgaccag
     1921 agcgccctga atctgcccca tcgacttacg ggtggcttct tttggtggca aaccttcttc
     1981 cgccatcaca cgctcaacgt tttctaccac aacgatggcg tcatccacca acaggccgat
     2041 ggcgagcacc atcccgaaca ttgttagcgt gtttatcgag aagccaaagg cggcaaggac
     2101 ggcaaaggtc ccgagcaata ccaccggtac ggcaatggtc ggaatcaacg tcgcgcggaa
     2161 gttctgcaag aacagataca ttaccaggaa cacgaggatg atcgcttcga ccagcgtttt
     2221 aaccacttcg tgaatagaga ttttcacgaa cggtgtggtg tcatacgggt aaacaatttt
     2281 cagacccgac gggaagaacg gttccatctt cgccagttca gcacggattg ccgcagcggt
     2341 atccagcgcg ttcgcaccgg tcgccaactt gatccccaga ccggaagccg gttggccgtt
     2401 aaactctgcg atgatgtcgt agttctcacc acccagctca attttcgcca catcacgcag
     2461 cagcacgcgg gaaccatcct gattcacttt cagcaggatt ttgccgaact cttcagtaga
     2521 ggtcagacgc gtctgagcaa taatagaggc gttaagctgt tggcctttca ccggcggcgt
     2581 accaccgagc tgaccagctg caacctgggc gttctgtgct ttgatggcgg taatgacatc
     2641 aaccggcgtt aactggaatt tgttcagttc attcgggttc atccagatac gcatcgcgta
     2701 ctgtgaaccg aacaactgaa cgtcacccac gcccgacgta cggctgatgg catctttcat
     2761 attcgccgcc acgtagtcgg agatatcctc ctgcgtcatg gtgccatcgg tgttgataac
     2821 gccgacaacc atcaggaagc tgctggatga tttctcaacg ctcacccctt gctgctgtac
     2881 ttcttgcggc agcaacggca tcgccagctg cagtttgttc tgcacctgaa cctgcgcgat
     2941 atccgcatca gtaccagact caaaggtcag ggtgatctgc acggtacccg tggagtcact
     3001 gttagaggac atgtacatca ggttatcgat accgttcata ttctgttcga taacctgtgt
     3061 caccgtgtcc tgcactgttt tcgcatcagc gccagggtag gaggcggaga tcgttactgc
     3121 cggcggtgca atcgtaggat attgcgccac cggcagtttg aggatcgcca gcccccctgc
     3181 caacatgatg ataatggcga tcacccacgc aaaaatcggg cgatcgataa agaaattagg
     3241 catgtcttaa cggctcctgt ttaagttaag acttggactg ttcaggctga gcaccgcttg
     3301 cggcttgctg gttattatca gcggtaactt cttgtgcttt tacctggaca ccaggacgca
     3361 ctttctgcag cccacttatt actacgcgat cgcctgcttt cagaccttct gtcaccagcc
     3421 acttatcgcc gatagcctgg cttgcaacga tcggacgggt ttccactttg tcatccgcgc
     3481 caaccaccag tacggtggca tcgccacgcg gcgtacgggt tacgccctgt tgcgggacta
     3541 aaatagcgtt tggattaagc ccttcttcca gacgtgcacg cacgaacata cccggcagca
     3601 gagtgtgatc cgggttcggg aagatagcgc gtagggtgat agacccagtg gtctgatcaa
     3661 cggtaacgtc agagaattcc agcgtaccgt cctgcgggaa cttaatgccg tcactggtga
     3721 tcagcgacac tttggctttg ccgttctctt gtttcagcgt gccattcgcc agttcctgtt
     3781 tcaggcgcag gaagtcgttg ctggactggg tcacatcaac gtagatcgga tcaagttgct
     3841 gcacggttgc cagcgcagtc gcctgaccgt tctgtaccaa tgcgccttcc gtcacgtttg
     3901 acttaccaat gcgaccacta atcggagagg tgactttggt gtaagccaga ttgattcgcg
     3961 aagtttcaac ggcagctttc gccgcagtta ccgcagcatt cgcctgttgc gcatcagcca
     4021 gagcctgatc gtactcttgc ttactgatgt actgagtacc gagcaatttc tgataacgat
     4081 tcaccgtcaa ttgcgcgata ttggctgcag cctgggcttt cgccagatca cctttcgcac
     4141 tgtcgtatgc cgcctgatag gtcgcaggat caatctgata gagagagaca cctgcttcga
     4201 tgtcgctacc ttctttgaaa ttacgcttca ggataatccc gctaacttga ggacgaactt
     4261 ctgcgatccg gtaggcactg gtgcgacccg gaagctcggt tgtgatctgc agaggttcag
     4321 ttttgactgt tactacgcca acggcgggca tctgctggcc accttgttgg gcctgtttgt
     4381 cgtcacatcc tgttagggct aagctgcctg agagcatcag aacgaccgcc agaggcgtaa
     4441 accctctgtt tttgttcata tgtaaacctc gagtgtccga tttcaaattg gtcaatggtc
     4501 aaaagttaat aaacccattg ctgcgtttat attatcgtcg tgctatggta catacattca
     4561 caaatgtatg taaatctaac gcctgtaaat tcacgaacat atggcacgaa aaaccaaaca
     4621 agaagcgcaa gaaacgcgcc aacacatcct cgatgtggct ctacgtcttt tctcacagca
     4681 gggggtatca tccacctcgc tgggtgagat tgcaaaagca gctggcgtta cgcgcggtgc
     4741 aatctactgg cattttaaag acaagtcgga tttgttcagt gagatctggg aactgtcaga
     4801 atccaatatt ggtgaactcg agcttgagta tcaggcaaaa ttccctggcg atccactctc
     4861 agtattaaga gagatattaa ttcatgttct tgagtccacg gtgacagaag aacggcgtcg
     4921 attattgatg gagattatat tccacaaatg cgaatttgtc ggcgaaatgg ctgttgtgca
     4981 acaggcacaa cgtaatctct gtctggaaag ttatgaccgt atagaacaaa cgttaaaaca
     5041 ttgtattgaa gcgaaaatgt tgcctgcgga tttaatgacg cgtcgcgcag caattattat
     5101 gcgcggctat atttccggcc tgatggaaaa ctggctcttt gccccgcaat cttttgatct
     5161 taaaaaagaa gcccgcgatt acgtcgccat cttactggag atgtatctcc tgtgccccac
     5221 gcttcgtaac cctgccacta acgaataacc ctgaatctga ctccaggaat tttcctggac
     5281 attttcgtcg ttgctattct ggttcactgc gtcgtgatat tcttgcggtt tgactttttc
     5341 aggtcgttct tcaggttcag aaaccttcat tcatcatgac tatgttccag tattacaaac
     5401 gatcacggca ttttgttttt tcagcattta ttgcttttgt ttttgtcttg ttatgccaga
     5461 acacggcgtt tgcacgggcg tcatcgaatg gtgatttacc gacaaaagcg gacctgcagg
     5521 cacaacttga ctcactgaat aaacaaaaag atctttctgc tcaggacaaa ctggtgcagc
     5581 aggatctgac agatacatta gccaccctcg ataaaatcga tcgcgtaaaa gaagagacag
     5641 ttcagctacg gcaaaaagtc gctgaagcgc cggaaaaaat gcgccaggcg accgcggcgt
     5701 taacagcact tagtgatgtc gataacgacg aagaaacgcg caaaattctg agcacgcttt
     5761 cgttgcgcca gctggaaact cgcgttgccc aggcgctgga cgatttgcaa aacgcacaaa
     5821 acgatctggc gtcttataac agccaactgg tttcgctaca gacgcagccc gaacgcgtac
     5881 aaaatgcgat gtataacgct tcgcagcagc tgcaacaaat tcgcagtcgt ctggatggga
     5941 ctgatgtcgg cgagactgcc ttacgtccca gccagaaagt gttaatgcag gtccagcagg
     6001 ctttgctgaa tgccgagatt gaccagcagc gtaaaagcct ggaagggaac actgtcttgc
     6061 aggatacctt gcaaaagcag cgtgattacg tgacggcgaa cagcgcccgt ctggagcacc
     6121 agttacaact gttgcaagaa gcggtaaaca gcaagcgcct gactttaacc gaaaaaacgg
     6181 cgcaggaagc tgtctccccg gatgaagccg cgcgtattca ggctaatccg ctggtgaagc
     6241 aggaactgga aattaaccag cagttaagtc agcgtctgat taccgcgact caaaacggta
     6301 atcagttgat gcagcaaaac attaaagtca aaaactggct ggagcgtgcg ctgcaatcgg
     6361 aacgtaatat taaggagcag attgccgttc tgaagggcag tctgctgttg tcacgtatcc
     6421 tgtaccagca acaacaaaca ctcccctcgg cagatgaact ggaaaacatg accaaccgca
     6481 tcgcggattt gcgtctcgaa cagtttgaag tcaaccagca gcgtgacgcg ctgttccaga
     6541 gcgatgcgtt cgtcagcaaa ctggaagaag gtcacaccaa cgaagtcaac agcgaagttc
     6601 acgatgcatt attgcaagta gttgatatgc gtcgcgaatt gctggatcag cttaacaaac
     6661 agttgggtaa ccagctgatg atggccatta acctgcaaat caaccagcag cagttaatga
     6721 gtgtgtcgaa aaacctgaaa tccatcctga cgcagcaaat tttttgggtg aacagtaacc
     6781 gcccgatgga ctgggactgg attaaagcgt tcccgcaaac gctgaaagat gaatttaagt
     6841 cgatgaaaat cacggtgaac tgggaaaaag cctggcccgc cgtattcatc gccttcctcg
     6901 ctggtttgcc gctgctgttg attgccgggc tgatccactg gcgtctgggc tggctgaaag
     6961 cgtatcaaca aaaactggct tccgctgtgg gttccctgcg taacgacagc cagctcaaca
     7021 caccaaaagc gattcttatt gacctaatcc gcgcactgcc ggtgtgcctg attattctcg
     7081 ctgtcggcct gattctgctg actatgcagc tcaatatcag cgagttattg tggtcgttca
     7141 gcaaaaaact ggcgattttc tggctggtat ttggcctgtg ctggaaagtg ctggagaaaa
     7201 atggtgttgc cgtgcgtcac tttggtatgc cggaacagca gaccagccac tggcgtcggc
     7261 aaattgtccg catcagcctc gcgttgctgc cgatccattt ctggtctgtg gtggcagaac
     7321 tctccccgct gcatctgatg gatgatgtgc tggggcaggc gatgattttc ttcaacctgc
     7381 tgctgattgc cttcctggtg tggccgatgt gccgcgaaag ctggcgtgat aaagagtcgc
     7441 acaccatgcg actggtcacc attaccgtgc tgtcgattat cccgattgcg ttgatggtgc
     7501 tgaccgccac gggttacttc tacaccacgc tgcgcctctc cgggcgttgg attgaaaccg
     7561 tttatttggt gatcatctgg aacctgctgt accagacggt attgcgcggc ttaagcgtag
     7621 cggcgcggcg tatcgcctgg cgtcgtgcac tggcgcgtcg gcagaatctg gtgaaagagg
     7681 gcgcagaagg tgccgaaccg ccggaagaac ccaccattgc gctggagcag gttaaccagc
     7741 agacgctgcg tattaccatg ttgctgatgt ttgcgctgtt cggtgtcatg ttctgggcaa
     7801 tttggtctga tttgatcacc gtgttcagct atctcgacag cattacgctc tggcattaca
     7861 acgctactga agctggtgct gcggtggtga aaaacgtcac catgggcagt ctgttgtttg
     7921 cgattatcgc ctcaatggtg gcctgggcgt tgattcgcaa cctgcctggt ttgctggaag
     7981 tgctggtgct ctcgcgactg aatatgcgcc agggggcatc gtatgccatc accactatcc
     8041 ttaactacat catcattgcc gttggtgcga tgacggtgtt cggatcgctg ggcgtctcgt
     8101 gggataaact ccagtggctg gcggcagcat tatccgtagg tcttggtttt ggtttacaag
     8161 agatcttcgg taacttcgtt tccggtttga tcattctgtt cgaacgtccg gtgcgcatag
     8221 gcgacacggt aaccattggt agcttctcgg gtacggtcag caagatccgt attcgtgcga
     8281 caacgattac cgatttcgat cgcaaagaag tgattatccc gaacaaagcg tttgttaccg
     8341 agcgtctgat caactggtcg ttaactgaca cgactacgcg tctggtgatc cgtctcggcg
     8401 tagcctatgg ctccgatctg gaaaaagtgc gtaaagtgtt gctgaaggcg gcgactgagc
     8461 acccaagggt gatgcacgaa ccaatgccgg aagtcttctt tacggcattt ggtgccagca
     8521 cgttggatca tgagctgcgt ctgtatgtgc gtgaactgcg tgaccgtagc cgtactgtcg
     8581 atgagctgaa ccgtactatc gatcagttgt gccgtgaaaa cgacatcaac attgccttta
     8641 accaacttga agtgcatctg cacaacgaga agggcgatga agtgacggaa gtgaaacgcg
     8701 actacaaagg cgacgatccg acgccagcgg tagggtaata accaaagggg gcgacattta
     8761 gttgccccga gattactaac aaagtgcgcg ttgttcatgc cggatgcggc gtgaccgcct
     8821 tatccggcct acgaaaccgc aagaattcaa tatattgcag gagcggtgta ggcctgataa
     8881 gcgtagcgca tcaggcagtt ttgcgtttgc ccacaatctt aggggacatt tagcggcccc
     8941 atttatttct cacttttccg cctcatcatc gcgcgttaat ttcttttcat aatcgcgttt
     9001 tacaatatcc agcgcgcgca gcacggtgcg ggctgggatc tgattttctt ccagtagcac
     9061 aatcaaatcg acggccagtt tgacatcgtc aggggcattt tccagtgaca tactctctcc
     9121 attgctagcg ggttaaacgc gctaacctgt tttcgatttt ttccagcgca tggcggcagc
     9181 gtgccaggcg cgcttcatag gcttccactt cacgatgcag cgtctgctgt tccacgagat
     9241 cggtcgctcg tgccagacgg gctctacgtt cggcaaccat ctcacgtagc cgccgctcaa
     9301 aatcctgatg ctgaatacgt ttacgctgcc agcgggcaat tttcggtggt gcgctatccc
     9361 actcgcgtag cgaccaggct gtggcttcac gcgctatagc ctccagttgc gccgccagat
     9421 gttctgccag ccaggcgact tgcggcagct gttgctgctc aaccgcatga cgaagcgcag
     9481 ccaggttatc gcccgcctca tcgagacaag cctgtagtgt tgtcgcacgg gtctgaaaaa
     9541 ggtgcctgtc aaaacgggca cttagcgtgg caaattgcgc taccggggca caacgctgac
     9601 gcagcgtggc gagctgtcct tccagttttt ccagtagcag ggtggttttc acgataagac
     9661 ctcaatgaaa atgataattg ttatgctaaa gtagccactc tgtaagctga caacactcaa
     9721 tatgcaacga atcattttaa tcatcattgg ctggctggcg gtagtgctgg gtacgctggg
     9781 cgtggtatta ccggtattac cgacgacgcc gtttatcctg ctggcggcct ggtgctttgc
     9841 ccgttcttcc ccgcgctttc acgcctggtt gctgtaccgc tcatggtttg gcagttatct
     9901 acgtttctgg cagaaacatc atgcgatgcc gcgcggcgca aaaccacggg cgattttgct
     9961 tattttgatt acgtttacga tttcgctgtg gttcgtccag atgccgtggg tgcgcatcat
    10021 gttgctggta attctcgcct gtttgctttt ttatatgtgg cgaattccgg tgattgatga
    10081 aaagcaagaa aagcactgaa gcacaacaat cgcagttgca attattgcgt acagccagta
    10141 cattctggcg ttttcgagca caggcgcagg cggtcaaagg ttaaacaact gttacttttg
    10201 atacgttcaa aacgcgccgt gagtaccacc gtaacaagca ggcatacact tatgaccgcg
    10261 actgcacagc agcttgagta tctcaaaaat agcatcaaaa gcattcagga ctacccaaaa
    10321 cccggcattc ttttccgcga tgtcaccagc ttactggaag acccgaaagc ttacgctctc
    10381 agcatcgact tgctggttga gcgttacaaa aatgcgggca ttaccaaagt ggtcggcacc
    10441 gaagcgcgtg gcttcttgtt tggcgctccg gtagctctgg gtctgggcgt tggctttgta
    10501 ccggtccgta aaccgggcaa actgccgcgt gaaaccatca gtgaaactta cgacctagaa
    10561 tacggcaccg atcagctgga aatccacgtt gatgccatca aaccgggcga taaagttctg
    10621 gtggtggacg atctgctggc aaccggcggc actatcgaag cgaccgttaa actgatccgt
    10681 cgtctgggtg gtgaagtagc tgacgctgcg ttcattatca acctgttcga tctcggcggt
    10741 gaacagcgtc tcgaaaaaca gggcattacc agctacagcc ttgtcccgtt cccgggccat
    10801 taattatcgt cagtcttgcg ctgctcacgc tacgggcagc acaagatgtg cattcagcct
    10861 cgccgttctg acggggctgt gttagcatta ccccttcgtg aatccacctt ccagcgtttc
    10921 agagcctgcc aatgagttat caggtcttag cccgaaaatg gcgcccacaa acctttgctg
    10981 acgtcgtcgg ccaggaacat gtgctgaccg cactggcgaa cggcttgtcg ttagggcgta
    11041 ttcatcatgc ttatcttttt tccggcaccc gtggcgtcgg aaaaacctct atcgcccgac
    11101 tgttggcgaa ggggctaaac tgcgaaaccg gcattaccgc gacgccgtgc ggcgtgtgcg
    11161 ataactgtcg tgaaatcgag caggggcgct ttgtcgatct gattgaaatc gacgccgcct
    11221 cgcgcactaa agtcgaagat acccgcgacc tgctggataa cgttcagtac gctccggcgc
    11281 gtggtcgttt caaagtttat ctcatcgacg aagtgcatat gctgtcgcgt cacagcttta
    11341 acgcactgtt aaaaaccctt gaagagccgc cagagcacgt taagtttttg ctggcgacga
    11401 ccgatccgca gaaattgccg gtgacgattt tgtcacgctg tctgcaattt catctcaagg
    11461 cgctggatgt cgagcaaatt cgccatcagc ttgagcacat cctcaacgaa gaacatatcg
    11521 ctcacgagcc gcgggcgctg caattgttgg cacgcgccgc tgaaggcagc ctgcgagatg
    11581 ccttaagtct gaccgaccag gcgattgcca gcggtgacgg ccaggtttca acccaggcgg
    11641 tcagtgcgat gctgggtacg cttgacgacg atcaggcgtt atcgctggtt gaagcgatgg
    11701 tcgaggccaa cggcgagcgc gtaatggcgc taattaatga agctgctgcc cgtggtatcg
    11761 agtgggaagc gttgctggtg gaaatgctcg gtctgttgca tcgtattgcg atggtacaac
    11821 tttcgcctgc tgcacttggc aacgacatgg ccgccatcga gctgcggatg cgtgaactgg
    11881 cgcgcaccat accgccgacg gatattcagc tttactatca gacgctgttg attggtcgca
    11941 aagaattacc gtatgcgcca gaccgccgta tgggcgttga gatgacgctg ctgcgcgcgc
    12001 tggcgttcca tccgcgtatg cctctgcctg agccagaagt gccacgccag tcctttgcgc
    12061 ccgttgcgca aacggcagta atgacgccaa cacaggtgcc gccgcaaccg caatcagcgc
    12121 cgcagcaggc accgactgta ccgctcccgg aaaccaccag ccaggtgctg gcggcgcgcc
    12181 agcagttgct gcgcgtgcag ggagcaacca aagcaaaaaa gagtgaaccg gcagccgcta
    12241 cccgcgcgcg gccggtgaat aacgctgcgc tggaaagact ggcttcggtc accgatcgcg
    12301 ttcaggcgcg cccggtgcca tcggcgctgg aaaaagcgcc agccaaaaaa gaagcgtatc
    12361 gctggaaggc gaccactcca gtgatgcagc aaaaagaagt ggtcgccacg ccgaaggcgc
    12421 tgaaaaaagc gctggaacat gaaaaaacgc cggaactggc ggcgaagctg gcggcagaag
    12481 ccattgagcg cgacccgtgg gcggctcagg tgagtcaact ttcgctacca aaactggtcg
    12541 aacaggtggc gttaaatgcc tggaaagagg agagcgacaa cgcagtatgt ctgcatttgc
    12601 gctcctctca gcggcatttg aacaaccgcg gcgcacagca aaaactggct gaagcgttga
    12661 gcacgttaaa aggttcaacg gttgaactga ctatcgttga agatgataat cccgcggtgc
    12721 gtacgccgct ggaatggcgt caggcgatat acgaagaaaa acttgcgcag gcgcgcgagt
    12781 ccattattgc ggataataat attcagaccc tgcgtcgatt cttcgatgcg gagctggatg
    12841 aagaaagtat ccgccccatt tgatcgtaag cacagcttac gttcgtcatc cttaacgtga
    12901 ttgagagaga aacctatgtt tggtaaaggc ggtctgggta acctgatgaa gcaagcccag
    12961 cagatgcaag aaaaaatgca gaaaatgcag gaagagatcg cgcagctgga agtcaccggc
    13021 gaatctggcg caggtctggt aaaagtgacc atcaacggtg cacacaactg ccgtcgcgta
    13081 gagatcgacc cgagcctgct ggaagacgac aaagagatgc tggaagacct ggtggctgca
    13141 gcattcaacg acgcagcacg tcgtattgaa gaaacgcaga aagaaaaaat ggcctctgta
    13201 tcctccggaa tgcagctgcc gcctggcttt aagatgccgt tctgatgcaa accagcccgc
    13261 tgttaacaca gcttatggaa gcactgcgct gtctgccggg cgttggcccg aagtcggcgc
    13321 agcgtatggc gttcacgctg cttcagcgcg atcgtagcgg cgggatgcgt ctggcgcagg
    13381 cgctcacccg ggcgatgtcg gaaatcggcc actgcgccga ttgccgcacc ttcaccgaac
    13441 aggaagtctg taacatctgt tcgaatccac gtcgtcagga aaacggtcaa atctgcgtgg
    13501 tggagagtcc ggcggacatt tacgccattg agcagacggg gcagttttca ggtcgttatt
    13561 ttgtgttgat gggacacctg tcaccgctgg acggcatcgg tcctgatgat atcgggcttg
    13621 atcgtctgga acagcgtctg gcagaggaaa aaatcactga agtgatcctc gccaccaacc
    13681 ccacggttga aggtgaagct accgctaact acattgccga gctttgtgcg caatatgacg
    13741 tggaagccag ccgaatcgct catggcgtac cggttggcgg cgaactggaa atggtcgatg
    13801 gcaccacgct gtcacactcc cttgccgggc gtcataagat tcgtttttaa gcaaacgaga
    13861 gcaggatcac ctgctctcgc ttgaaattat tctcccttgt ccccatctct ccctcatcct
    13921 gtttttaacc ttaaaatggc attattgagg tagacctaca tgaaaggaca agaaactcgt
    13981 ggttttcagt cagaagtgaa acagcttctg cacctgatga tccattctct ctattccaat
    14041 aaagaaatct tcctgcgtga gcttatctct aacgcctccg atgcggcgga caagctgcgt
    14101 ttccgcgcgc tttctaaccc ggacctgtac gaaggtgatg gtgaactacg cgttcgtgtc
    14161 tctttcgata aagacaagcg cacgctgacc atctccgata acggcgtggg gatgacccgc
    14221 gacgaagtga ttgaccatct ggggactatc gctaaatccg gcaccaaatc attcctcgaa
    14281 tccctgggtt ctgaccaggc gaaagacagc cagctgatcg gtcagtttgg tgttggtttc
    14341 tactctgcgt ttatcgtggc tgacaaagtg accgtgcgta ctcgcgcggc aggcgaaaaa
    14401 ccagaaaacg gcgtcttctg ggaatcggct ggcgaaggtg aatacaccgt tgccgacatc
    14461 actaaagaag atcgtggtac tgaaatcacc ctgcatctgc gtgaaggcga agacgagttc
    14521 ctcgatgact ggcgtgtgcg ttccatcatc agcaaatatt ccgaccatat cgcgctgccg
    14581 gtagagatcg aaaaacgcga agagaaagac ggcgaaaccg ttatctcctg ggagaaaatc
    14641 aacaaagcgc aggcgctgtg gactcgtaac aagtcggaaa tcaccgatga agagtacaaa
    14701 gagttctaca aacacatcgc ccacgacttt aacgatccgc tgacctggag ccacaaccgc
    14761 gttgaaggta agcaggagta caccagcctg ctgtacattc cgtctcaggc accgtgggat
    14821 atgtggaacc gcgatcataa acacggtctg aaactgtatg ttcagcgtgt gttcatcatg
    14881 gacgacgcag aacagttcat gccgaactat ctgcgcttcg tgcgtggtct gattgactcc
    14941 agcgatctgc cgctgaacgt ttcccgtgaa atcctccagg acagcacggt aacgcgtaac
    15001 ctgcgcaatg cgctgaccaa gcgtgtgctg caaatgctgg aaaaactggc gaaagacgac
    15061 gcggaaaaat accagacctt ctggcaacag tttggcctgg tactgaaaga aggtccggcg
    15121 gaagatttcg ctaaccagga agcgatcgcc aaactgctgc gttttgcttc tacccatacc
    15181 gattcttctg cgcagaccgt gtctctggaa gactacgttt cccgcatgaa agaagggcag
    15241 gagaaaatct actatatcac cgcagacagt tacgcggcag cgaagagcag cccgcacctg
    15301 gaactgctgc gtaagaaagg catcgaagtt ctgctgcttt ccgaccgcat cgatgagtgg
    15361 atgatgaact atctgactga gttcgacggt aaaccgttcc agtctgtgtc taaagttgac
    15421 gagtcgctgg aaaaactggc tgacgaagtt gatgagagcg cgaaagaagc ggagaaagca
    15481 ctgactccgt tcatcgaccg tgtgaaagcc ctgctcggcg agcgcgtgaa agatgtccgt
    15541 ctgactcacc gtctgaccga tacgccagcg atcgtttcga ccgacgcgga cgaaatgagc
    15601 actcagatgg cgaaactgtt cgctgcggcg ggccagaaag tgccagaagt gaaatacatc
    15661 ttcgaactga acccggatca cgtactggtg aaacgtgcgg cggatactga agatgaagcg
    15721 aagttcagcg agtgggtaga actgctgctg gatcaggcgc tgctggcaga acgcggcacg
    15781 ctggaagatc cgaacctgtt tattcgtcgt atgaaccagc tgctggtttc ctgatgtcat
    15841 gccgggtgac cttcgtgtca tccggcattt ttcttttctt catctgcact ttccgcaaat
    15901 tatcttgcca ttaaccgttt cagcctcagg tgcctttctt gaggcaatcg cctgttggtg
    15961 gtatcgttta tcgctttttc aaaaaattcg acacatttta aggggatttt cgcaatgcgt
    16021 atcattctgc ttggcgctcc gggcgcgggg aaagggactc aggctcagtt catcatggag
    16081 aaatatggta ttccgcaaat ctccactggc gatatgctgc gtgctgcggt caaatctggc
    16141 tccgagctgg gtaaacaagc aaaagacatt atggatgctg gcaaactggt caccgacgaa
    16201 ctggtgatcg cgctggttaa agagcgcatt gctcaggaag actgccgcaa cggtttcctg
    16261 ttggacggct tcccgcgtac cattccgcag gcagacgcga tgaaagaagc gggcatcaat
    16321 gttgattacg ttctggaatt cgacgtaccg gacgaactga ttgttgaccg tatcgtaggc
    16381 cgccgcgttc acgcgccgtc tggtcgtgtt tatcacgtta aattcaatcc gccgaaagta
    16441 gaaggcaaag acgacgttac cggtgaagag ctgactaccc gtaaagacga tcaggaagag
    16501 accgtacgta aacgtctggt tgaataccat cagatgactg caccgctgat cggctactac
    16561 tccaaagaag cggaagcggg taacaccaaa tacgcgaaag ttgacggcac caagccggtg
    16621 gctgaagttc gcgcagctct ggaaaaaatt ctcggctaat caagccgccg cagatctgct
    16681 ctcgggcagg tctgctcatc ctcctcaggc aatacctctt acagcaatta gttcttcttc
    16741 ttcacttttc cgctacaatt atcaacaagt tgaatcgata agaggcggta atgcgtcaaa
    16801 ctaaaaccgg tatcctgctg gcaaacctgg gtacgcccga tgcccccaca cctgaagcgg
    16861 taaaacgcta tctgaaacaa tttttaagcg acagacgcgt ggttgatacc tcacggttgt
    16921 tatggtggcc gttgctgcgc ggcgtgattt tgccgctgcg ttcgccgcgt gtggcgaagc
    16981 tgtatgcctc tgtctggatg gaaggtggct cgccgctgat ggtttacagc cgccagcaac
    17041 agcaggcgct ggcgcaacgt ttaccggaga cgcccgtggc gctgggaatg agctacggct
    17101 cgccatcact ggaaagcgcc gtagatgaac ttctggcaga gcatgtagat catattgtgg
    17161 tgctgccgct ttatccgcaa tactcctgtt caacggtcgg tgcggtatgg gatgaactgg
    17221 cacgcattct ggcgcgcaaa cgtagcattc cggggatatc gtttattcgt gattacgccg
    17281 ataaccacga ttatattaat gcactggcga acagcgtacg tgcttctttt gccaaacatg
    17341 gcgaaccgga tctgttgttg ctctcttatc atggcattcc ccagcgttat gcagatgaag
    17401 gcgatgatta cccgcaacgt tgccgcacaa caactcgcga actcgcttcc gcactggaga
    17461 tggcaccgga aaaagtgatg atgacctttc agtcgcgctt tggccgggaa ccctggctga
    17521 tgccttatac cgacgaaacg ctgaaaatgc tcggagaaaa aggcgtgggt catattcagg
    17581 tgatgtgccc gggctttgct gcggattgtc tggagacgct ggaagagatt gccgagcaaa
    17641 accgtgaagt cttcctcggc gctggcggga aaaaatacga atatattccg gcgcttaatg
    17701 ctacaccgga gcatatcgaa atgatggcta atcttgttgc agcctatcgc taaagaatcg
    17761 agcaccgtcg cgaagagcct cgtcggcggt tttcatcatc cgtgaataat gcaaaaaggc
    17821 gtgcagcgtg cctgggtaga gtttgaactc acagggctgc tgatgcgccg ctaacgtctg
    17881 gtaaagcaga cggctgtcat ccagcagtgg atcgaactcc gccccggcaa taaaacaggg
    17941 cggaacttcg cgagtgagat cattattaaa caggcagtaa tatggcgact cgcggtccgc
    18001 gtcgttgctt aaatacgcct cttcatacat ctgcaaatcc tgttgcgtta agccatccca
    18061 gacaccaccc aacagacgac gagtcacgga atcccgtaat ccgtaaagcc cataccacag
    18121 caaaacgccc gcaactttac cgcaatcgat ctgtttatcg cgcaaccaca acgcactggc
    18181 gagcgccagc atggcacctg cggaatcacc agcaaaacca atgcgggaca tattgatttg
    18241 ataatcctcc gcctgctggt ggaaataaca acaggcagcc acaatttcct ctatcgcttg
    18301 cggaaaacgc gcttcaggtg aaagggtgta atcaatgcca atcaccgtac attggctgta
    18361 gcttgccagc aggcgcatga tgcgatcgtg ggtatcgaga ttgccgagaa taaaaccgcc
    18421 tccatgcaaa taaaatagcg tcgccgggct atctggctgc ggataaaaga tacgtgtttt
    18481 cacctgccca tattttgttg gaaccctgta agctctggtt gccatttctg gagcgcccac
    18541 attccagaat cggcgctcaa gcgtgtaata ctgtcgttgc tcagcaatcg ttcccgttgc
    18601 aggccagggc ggtaaatccg gctgaagagt attcacaacg gtcttcattt cagcagagat
    18661 aaggtccaga acgggtaatt tgttttccgg cttcatgcaa aaactccttt caaattacgt
    18721 cattgtaagg aaccactgcc atgaaaatgc gatcccgcct gctgatattg aaactggctg
    18781 cgtctcgcgc gctcccgtca gattgtgtta acattcgccg ctcagttaac cacccgtaaa
    18841 aacaaccatg aaatttcccg gtaaacgtaa atccaaacat tacttccccg taaacgcacg
    18901 cgatccgctg cttcagcaat tccagccaga aaacgaaacc agcgctgcct gggtagtggg
    18961 tatcgatcaa acgctggtcg atattgaagc gaaagtggat gatgaattca ttgagcgtta
    19021 tggattaagc gccgggcatt cattggtgat tgaggatgac gttgccgaag cgctttatca
    19081 ggaactaaaa cagaaaaacc tgattaccca tcagtttgcg ggtggcacca ttggtaacac
    19141 catgcacaat tactcggtgc tcgcggacga ccgttcggtg ctgctgggcg tcatgtgcag
    19201 caatattgaa attggcagct atgcctatcg ttacctgtgt aacacctcca gccgtaccga
    19261 tcttaactat ctacaaggcg tggatggccc gattggtcgt tgctttacgc tgattggcga
    19321 gtctggggaa cgtacctttg ctatcagccc gggccacatg aaccagctgc gggctgaaag
    19381 tattccggaa gatgtgattg ccggagcctc ggcactggtt ctcacctctt atctggtgcg
    19441 ttgcaagccg ggtgaaccca tgccggaagc aactatgaaa gccattgagt acgcgaagaa
    19501 atataacgta ccggtggtgc tgacgctggg caccaagttt gtcattgccg agaatccgca
    19561 gtggtggcag caattcctca aagaccacgt ctctatcctt gcgatgaacg aagatgaagc
    19621 cgaagcgttg accggagaaa gcgatccgtt gttggcatct gacaaggcgc tggactgggt
    19681 agatctggtg ctgtgcaccg ccgggccaat cggcttgtat atggcgggct ttaccgaaga
    19741 cgaagcgaaa cgtaaaaccc agcatccgct gctgccgggt gctatagcgg aatttaatca
    19801 gtatgagttt agccgcgcca tgcgccacaa ggattgccag aatccgctgc gtgtctattc
    19861 gcacattgcg ccgtacatgg gcgggccgga aaaaatcatg aacactaatg gagcagggga
    19921 tggcgcactg gcagcgttgc tgcatgacat taccgccaac agctaccacc gtagcaacgt
    19981 accaaactcc agcaaacata aattcacctg gttaacttat tcatcgttag cgcaggtgtg
    20041 taaatatgct aaccgtgtga gctatcaggt actgaaccag cattcacctc gtttaacgcg
    20101 cggcttgccg gagcgtgaag acagcctgga agagtcttac tgggatcgtt aagttatcgt
    20161 cggttcgtag gccagataag gcgttcacgc cgcatctggc attttgcttt caatgcctga
    20221 tgcgacgctg gcgcgtctta tcaggcctac atatccgcgc ctattatccg gcaaccaccg
    20281 tttacctcgt caccacctca cccgccggtg gcgtttccag cagttccagc atagtacggg
    20341 caatttcacg ctcacccatc actacctgat tcgcaccacg ttcggtgata tacgccactt
    20401 catcgtcata atgggcgcgg gcaataatct caatatccgg atttttcgcg cgggcagatg
    20461 ccacaatctc gcccgcttca tagccgttgg gaatcgtcag gatcagccat tttgcacatt
    20521 ccagatgcgc cagttgcata atttcttcgt tcgccgcatt gcccaatact gcgcggaccc
    20581 cgcgctcgcg cagctcatca acacgggttc gtgacgtctc aatcaccacc agcggaatat
    20641 cagaggcgag caatttctcc cccagcaggc tgcctacacg accgtaaccc accagtagcg
    20701 catggttgca aatatccact gggatctgct tctcttcttc gattgcctct tccagcgtct
    20761 gctcttccag cgtttcggtc ttcgccagat atttttccag tagtgcgaac agtaccgggt
    20821 tgagcataat cgacaggatc gcccctgcca gtaccaggtt ttgtccggcc tgtggcagta
    20881 aatttaatgc cattcctagt cccgccagga taaacgcgaa ctcaccaatc tgcgccaggc
    20941 tggcggcgat ggttaacgcc gtgcgctggg agtgaccaaa caaacgcaca aggaacaggg
    21001 ccgcgaccga tttaccaaac agaataattg ccagcgtcgc cagtactgcc agcggttgct
    21061 gaatcagaat taacggatca aacaacatcc cgacggagac aaaaaacagc accgcaaacg
    21121 cgtcgcgcaa tggcagtgta tcgtgggcgg cacggtgact cagttcagac tcgttcagta
    21181 ccatcccggc aaagaacgca ccgagtgcaa aggagacatc aaacagctct accgcaccaa
    21241 aggcaatccc taacgccagc gccagcaccg acagggtaaa cagctcgcgg gaaccggttg
    21301 ctgcgctgcg tgccataatc cacggcacca gacggcgacc taccagcatc ataatggcga
    21361 taaatgcgat caccttgccg atggtgatcc ccatatcgac tgcaagagtg gcaaagccca
    21421 catcgccctg ttccatcatt cctgctactg cgggtagcaa taccagcgtc aggaccatca
    21481 ccaggtcttc cacaatcaac caaccgatgg cgatttgccc acgctgactg tcaattaatt
    21541 gccgttcttc aagtgcgcgc agtaacacca cggtactggc ggtggaaaga cataagccga
    21601 acacgatacc ggtcattaac gaccagccca gcacggcgga gagcgccata cccagcagcg
    21661 tcgccacggc tatctgggcg atcgcgccgg gaatggcgat ggactttacc gccatcaaat
    21721 ccttgagcga aaaatgcaaa ccgacgccga acatcagcaa aataacgccc agttcagcca
    21781 gttccggcgc aagcttggta tcggcaacaa agcccggagt gaatggtcct gccagcacac
    21841 ccgctaacag atatcccacc agaggagaaa tacgtagttt attggccagc atgccgagga
    21901 taaaggcgag cacaaggccg ccaacaatgg tggtgataag cggggtggcg tgatgcattc
    21961 cgtctccttt tcctggtggt tattgtccat ttttggccgg gaaaaccaaa attacaggta
    22021 atagtttatg acaatttcat tgatgatgtt tatgaataat tgttgaattt tgcagaaaaa
    22081 tggaattagc tgcaaaaaaa agcacggatc ggaaaacaga aggcggttta acagaggaaa
    22141 ggcttatggc gactgtaaaa cgatgcagcc aaagtttgcc tttggctgca atgaaatcag
    22201 tctttatgcc ggttatcagg caggaatatg gtcaacatcc ccaatagtgg caggaaagca
    22261 cagattttat agactaactc tatgctggtg tggtcggcga taagccccag aactgccgcc
    22321 cccagacctc ccatgccgaa agcaaaaccg aaaaacagtc cagaaaccat accgatacgt
    22381 cccggaagca gctcctgggc gtagaccaga atggcagaga atgctgaagc gaggataaat
    22441 ccaataatca ccgttaaaac ccccgtccag tgcaggctgg cgtagggtaa aatcagcgta
    22501 aacggcgcaa cgccgaggat agagccccaa atcacatatt tccgtccaat tttatcccct
    22561 acaggcccgc caatcaccgt acctgccgca acggcaaaca ggaaggcaaa caaatgaagc
    22621 tgagcattct ggatagataa tccgaatttt tgcatcagat aaaaggtgta atagctgctg
    22681 atgctcgcca tatagaaata tttcgagaaa atgaggatta acagaatgct gaccgccaga
    22741 acaactttat tgcgcggcag tggattgata atcgtcgctt tgggttttcc tttattcatt
    22801 cggtgctgtg ccgagtacca acggctgatt tgcgccaaca ccacgatcgc cagcagtgcc
    22861 gcaagcacaa accaggcaac gttgcctttg ccataaggcg cgataatcac cgccgccagc
    22921 aaggggccca gggaactgcc aaagttgccg ccgacctgaa agatagattg cgccaggcca
    22981 tgacgcccgc cggaagccat acgggccacg cgagaagatt ccggatgaaa gaccgatgaa
    23041 ccggtaccga ccagcgccgc tgccagcaga actgcgccga aactgcccgc cagcgccagc
    23101 agcaccagac cacttaaggt aaagcacatg ccaatcggca acgaccacgg catcggatat
    23161 ttatcggtcc agtagccgac caccggttgc agtagcgaag aggcgagctg gaaggtgagg
    23221 gttatcatgc caatctgcat aaatgtcaga gaaaattctg actgaagcag tggataaatc
    23281 gccagaatca gcgattggat catgtcgttc agcagatgtg agaggctgat agcacctaaa
    23341 ataccaaacg atgttcgggc cttagtcgtt gacgcagccg cgcccgccac aggctgggtt
    23401 tgttcactca ttgccatagg aaagtcactt tttcagggtt gcgatgtaaa gaatgatctt
    23461 atttgtgatt attacccgac taacatacct gtatgcgccg cctgaaggaa gtctcaacgc
    23521 ggaatacaga atttctaatc tggatgcaga tttatcttca ccggacgcag acttgtctat
    23581 gatgtcgcgt catactattt ttcaacacgt tgaaatcagg tcagggagag aagtatgaaa
    23641 ttattacagc ggggcgtggc gttagcactg ttaaccacat ttacactggc gagtgaaact
    23701 gctctggcgt acgagcagga taaaacctac aaaattacag ttctgcatac caatgatcat
    23761 catgggcatt tttggcgcaa tgaatatggt gaatatggtc tggcggcgca aaaaacgctg
    23821 gtggatggta tccgcaaaga ggttgcggct gaaggcggta gcgtgctgct actttccggt
    23881 ggcgacatta acactggcgt gcccgagtcc gacttacagg atgccgaacc tgattttcgc
    23941 ggtatgaatc tggtgggcta tgacgcgatg gcgatcggta atcatgaatt tgataacccg
    24001 ctcaccgtat tacgccagca ggaaaagtgg gctaagttcc cgttgctttc cgccaatatc
    24061 taccagaaaa gtaccggcga gcgcctgttt aaaccatggg cgctgtttaa gcgtcaggat
    24121 ctgaaaattg ccgttattgg cctgacgacg gatgacacag ccaaaattgg taatccggaa
    24181 tatttcactg atatcgaatt tcgtaagccc gctgatgaag cgaagctggt gattcaggag
    24241 ctgcaacaga cagaaaagcc agacattatt atcgcggcga cccatatggg gcattacgac
    24301 aatggtgagc acggctctaa cgcaccgggc gatgtggaga tggcacgcgc gctgcctgcc
    24361 ggatcgctgg cgatgatcgt cggtggtcac tcgcaagatc cagtctgtat ggcggcagaa
    24421 aacaaaaaac aggtcgatta cgtgccgggt acgccatgca aaccggatca acaaaacggc
    24481 atctggattg tgcaggcgca tgagtggggc aaatacgtgg gacgggctga ttttgagttt
    24541 cgtaatggcg aaatgaaaat ggttaactac cagctgattc cggtgaacct gaagaagaaa
    24601 gtgacctggg aagacgggaa aagcgagcgc gtgctgtaca cccctgaaat cgctgaaaac
    24661 cagcaaatga tctcgctgtt atcaccgttc cagaacaaag gcaaagcgca gctggaagtg
    24721 aaaataggcg aaaccaatgg tcgtctggaa ggcgatcgtg acaaagtacg ttttgtacag
    24781 accaatatgg ggcggttgat tctggcagca caaatggatc gcactggtgc cgactttgcg
    24841 gtgatgagcg gaggcggaat tcgtgattct atcgaagcag gtgatatcag ctataaaaac
    24901 gtactgaaag tgcagccatt cggcaatgtg gtggtgtatg ccgacatgac cggtaaagag
    24961 gtgattgatt acttgaccgc cgtcgcgcag atgaagccag attcaggtgc ctacccgcaa
    25021 tttgccaacg ttagctttgt ggcgaaagac ggtaaactga acgacctgaa aatcaaaggc
    25081 gaaccggtcg atccggcgaa aacttaccgc atggcgacat taaacttcaa tgccaccggc
    25141 ggggatggct atccgcgcct tgataacaaa ccgggctatg tgaataccgg ctttattgat
    25201 gccgaagtgc tgaaagcgta tatccagaaa agctcgccgc tggatgtgag tgtttatgaa
    25261 ccgaaaggtg aggtgagctg gcagtaatcc gaaagtgccg gatgttcgca tccggcacaa
    25321 tgcttaatcg cgacgggcga tatcagcaaa tttggcatcg agtatctttg ccaggtcgct
    25381 tgccgccagt tcgatatcca gaccgcgctt gccgccggaa acataaatag tggcaaattc
    25441 ttgtgcgggg gcgtcgataa tcgttggcag acgttttttc tgccccagtg ggctaatccc
    25501 tccaaccaga tatcccgtcg aacgctgcgc gaccatcgga tcggccatct caactttctt
    25561 ggcacccagc gcttttgcta cttttttaag atccagttga cctgcgaccg gcgtaacagc
    25621 cacggcaagg tgtttcatat caccgttcac tgcgaccagc agcgttttgt agacctgatc
    25681 cggattcaaa cctaattttt tgacgacttc atcgccaaaa ttggtttcag ccggatcgtg
    25741 ctcgtaggtg tggatttgaa acgaaatctt gtttttttcg agtaatttaa ctgcgggtgt
    25801 catggtaatt ttcctgcctt aaacaaaaaa acgaagcaag catacgcctg atggcggtcc
    25861 aaaaaaaaga gtcatcttgc ctaagagtat tggcaggatg gtgagattga gcgacaatcg
    25921 agttacaccg tcgcttaaag tgacggcata ataataaaaa aatgaaattc ctctttgacg
    25981 ggccaatagc gatattggcc atttttttag cgcaacatct gcggcaaatt cccttctcca
    26041 tacaggtgta gtgcaccaac cgcgaccaca tagcgccccg gcggcatggc gcgtaatttg
    26101 tcccgccagg cgagatttcg ctgatgcatc agcacatcgt acagcgactg actgaacgta
    26161 ttgggtagcg ttatttcatt attttgtggc ggtgtattca gccaccagct catcatttgt
    26221 tgcagcaacc gtgcgttggt gtgccagtgg gtcagcgtgt cgtccagcag tgccagtcct
    26281 ttgtcaggga gctggagcaa catggcaatc tggttttctg ccccttccag ttcaatcacg
    26341 ggtttatgtt gttgcttcgc cgcctgcaat agctggtaat caataccata ttctgcccgc
    26401 agccccagtt tttgtgcctg cgtcgcctga agaaccatcg cgatttgcca caacggttga
    26461 gtagaaaaga gtgacggaga aatgcccatc ttctggctaa tgtgctgcag gttttggagt
    26521 tgctcctcgc taatgcgctc ttccagtgcc tcgcaggcag gcaaattggc aaaaggcgta
    26581 tcgctggtgg aaacatccgc ctcgacgatc agcgcatcgg cgtttttgag ctttttgagc
    26641 aaacgggtgg gcaggggagc catatcgtgg ctacccatat gaatactgcc gatcagatga
    26701 aaatggcgat tgccgggaag agagatatcg atagcaggcc aggtgtaatg attaccgcgc
    26761 agcgcggccc aaagcgtttt tacccggtac aacagatcca tacgacctcc ctttgtgaaa
    26821 tatcatgcta gcgcgcgggg agggatggcg caaatggagt tgattgttgc acttgcgcac
    26881 agtgcttgtg tgcattacaa tgattaaata atggacaagg agatgagaga ggaatggctc
    26941 aaaagaaaaa tataaggagt tttcgtgatg cctggttggc tgattttttt gtccattcaa
    27001 cgccacatcg aaagatccct gctgaaatcc ataccacgtt atcacgaaag ttggacatca
    27061 ttaatgctgc cacttctcat cgggatttac gctcccctcc ggggaaccga tacgaagagt
    27121 tatccgggaa attacaggaa tactcctcca ttagagtgaa taaacagtac cgactgattt
    27181 ttaaatgggt taatggcaag gccgaagaat tgtttttaga cccacacaac tactaaaaca
    27241 tagctgtcgt attgacgaga aaggtaaaac tatgaaacag gcaacaagaa aaccgacgac
    27301 acccggagat attcttctct atgaatatct ggaaccgctc gatttgaaaa tcaatgagtt
    27361 agcagagttg ctgcatgttc atcgtaatag cgtcagtgca ctgatcaata acaatcgtaa
    27421 actcaccact gagatggcat tccgtctggc gaaagttttt gataccacag tcgatttttg
    27481 gctaaacctc caggcggcag ttgatctttg ggaagttgaa aacaacatgc gtacccagga
    27541 agaattggga cggattgaaa cagtggctga atatttggca cgccgtgaag agcgtgcaaa
    27601 aaaggtcgcg tagaataatt tccctataac ggatgcgcca ccctaaagca gcgcatccgc
    27661 aatgatgtac ttattccttc ggtttaaacc gcagcaagcg gttggcgtta ctcactacgg
    27721 tgatcgacga gagcgccatt gccgctccgg caaccaccgg gttaagcagt gttccagtga
    27781 acggccacaa aataccggcg gcgaccggaa taccaatgct gttgtagata aacgcaccga
    27841 gcaggttctg cttcatgttg cgcagcgttg cgcgggaaat tgcgagcgca tccgcaacac
    27901 ccatcaggct atggcgcatc agggtaatcg ccgcggtttc aatggcaaca tcactgccgc
    27961 cacccatcgc aataccgaca tccgcctgag ccagcgctgg cgcatcgtta atgccgtcgc
    28021 ccaccattgc tacctgacgt ccttcacttt gcagacgttt gatcgcttcg gctttaccgt
    28081 ccggcagcac gccggctatc acctcatcaa tccctgcttc tttagcgatc gcattggcgg
    28141 tggtcgggtt atccccggtt agcatcacca ggcgatatcc cgctttatgc aggcgttgca
    28201 gcgccgccac gctatcacta cgcaacggat cgcgtacagc cagcagggct accgctttcc
    28261 cgtcaaccgc cagcagcaca ggcgttgccc cttgcgatgc ctgagctgaa atatctgctt
    28321 caattgcttt ggtatcgacc tgttggtcat ttaacagtgc ctgattgccc agcaataacg
    28381 catgaccttc agcttcaccg ctcacgccca gcccgcgcaa tgtgcggaaa ccgttgacct
    28441 gcggtagctg catatcacct gctttatcga ggatcgcccg tgccagaggg tggctggaac
    28501 cttgctccag cgccgccgcc agacgcaatg cttgcgcttc atcaatatca gcaaatgttt
    28561 ttactgcgac aacctgtggc ttcccttcgg tcagcgtccc ggttttatcg aacactacgg
    28621 tatcgagcgt actggcgcgt tgcagcgcgt cggcgtcccg taccagcacg ccaaactcag
    28681 ccgcccgccc gacgcccgaa ataatcgaca tcggcgtcgc cagccccagc gcacacggac
    28741 aggcaataat tagcaccgtg gtggcaatca ccagggtata gacaatctgc ggtgccggac
    28801 caaagaaata ccagattgcc gcactgatca gcgcaatcac caccactacc ggcacaaata
    28861 cggctgagat tttatccgcc agctgaccga tttctggctt actgctctgg gcctggcgca
    28921 ccatacgaat aatccttgat agcgtggtat ggctgccaac cgcactggcg cgaaacaaca
    28981 cgctaccgtc ctgcaccact gtcccggcat ggacgctatc gccttcgcct ttttgctgcg
    29041 gtataggttc gcccgtcagc atcgcttcat ccagccacgc ttcgccctgg gtgatctcac
    29101 catctaccgg tacgcgatcg ccggtcgtca ggcgcagcaa catacctggc tgcacttccg
    29161 ccagcggcac gcttttttca ccttcgtcag taaccaggcg cgccgtcggc ggagttaaat
    29221 cgagtaattt ctccagcgcc tttgaagaac gctgacgtgc gcgcgcttcc agcatatgac
    29281 cgagattaat cagaccgata atcatcgcac tggcttcata gtaaagatgc cgtgcttcca
    29341 tcgggaacca ctgcggccac aggttgacgc tcatcgaata gagccacgca acacccgttc
    29401 ccagcgccac cagcgtatcc atcgtcgccg caccgttcag caggcttttc catgcgctgc
    29461 ggtaaaaatg cccgccggcg aaaaccatta ccgccagggt tatcaggccg ataactaacc
    29521 acaggctgcg gttgtcagcg gtgaccatca tgttatcgcc gatcatcccc cagaccatca
    29581 ccgggatacc caccgccagc gcgacaattg cctgccagcg gaagcgcttc atcgtagcga
    29641 cggcggtttc ttgctggcgt tcgcggcgtt tagcgtcatc ttcaatcgct tccgcgccgt
    29701 agcccgcttt ttccaccgcc tgcactaaat cttgtgggga ggcgctgccc atcaccagcg
    29761 cagtacgctc cgccaggttt acccgtgcct gagtgacgcc cggtacgctt tgcaacgcat
    29821 tttgtacgcg ggtgacacag ctggcgcagc tcatgccgct cagcagcaac tgctggctgt
    29881 catcgtcatc ggcggtcgct gccggaagcg cctcagaaac cgctgtcagt gcttccgacg
    29941 ggattgatga ctccgccagc ggtttagcct ttgggtggct tacagatgcg tcataacccg
    30001 cttgtttgat ggtttcgatt agctgttctg cactggcagt cccggtaacg tgcgcttcag
    30061 tgatagacac atccgcctgt tcaacatccg ggcgctgttc gagactttct ttcacgcgtt
    30121 taacgcagtg accgcaggac aggccgtcca gggtcaggtc gatagtttgt gacataaaac
    30181 actcctttaa gacagttttg actggctgtg ataaaggtta aaccttccag caaggggaag
    30241 gtcaagaaat taataaacca ggcgggtaaa agtccgtaaa gattaaaaaa tcggctcgat
    30301 ttgcatcagg attagacatt tatctctttg ttttcctgta gttaagttgc gggtgctaag
    30361 ttaaatcagg atgcatgaaa atcggcaccg gggtgaggaa ttacctcccg catctataaa
    30421 aaggagttaa caaaagatgt tagatgcaaa caaattacag caggcagtgg atcaggctta
    30481 cacccaattt cactcactta acggcggaca aaatgccgat tacattccct ttctggcgaa
    30541 tgtccctggt caactggcgg cagtgtctat cgtgaccagc gatggcaacg tctatagtgc
    30601 gggtgacagt gattaccgct ttgcgctgga atccatctcg aaagtctgta cgttagccct
    30661 tgcgttagaa gatgtcggcc cgcaggcggt acaggacaaa gttggcgctg acccgaccgg
    30721 attgcccttt agctcagtta tcgccttaga gttgcatggc ggcaaaccgc tttcgccact
    30781 ggtaaatgct ggcgctattg ccaccaccag cctgattaac gctgaaaata ctgaacaacg
    30841 ctggcagcga attttacata tccaacaaca actggctggt gagcaggtag cgctctctga
    30901 tgaagtcaac cagtcggaac aaacaaccaa cttccataac cgggccattg cctggctgct
    30961 gtactccgcc ggatatctct attgtgatgc aatggaagcc tgtgacgtgt acacccgtca
    31021 gtgctccacg ctcatcaata ctattgaact ggcaacgctt ggcgcgacgc tggcggcggg
    31081 tggtgtgaat ccgctgacgc ataaacgcgt tcttcaggcc gacaatgtgc cgtacattct
    31141 ggccgaaatg atgatggaag ggctgtatgg tcgctccggt gactgggcgt atcgcgttgg
    31201 tttaccgggc aaaagcggtg taggtggcgg tattctggcg gtcgttcctg gcgtgatggg
    31261 aattgccgcg ttttcaccac cgctggacga agaaggcaac agtgttcgcg gtcaaaaaat
    31321 ggtggcatcg gtcgctaagc aactcggcta taacgtgttt aagggctgat catgatgaac
    31381 acggaaggta ataacggtaa caaacccctc ggtctatgga acgtcgtttc catcggcatt
    31441 ggggcaatgg tgggggcggg gatcttcgcg ctgctggggc aggctgcgtt gctaatggaa
    31501 gcctcgacct gggtcgcctt tgcttttggc ggtattgtgg cgatgttttc cggttatgcc
    31561 tatgcgcgtc tgggggcgag ctatcccagc aatggcggta ttatcgactt ctttcgtcgc
    31621 ggattaggca acggcgtttt ttcgctggcg ctctcgttat tgtacctgtt gacgctggcg
    31681 gtgagtatcg ccatggtcgc ccgtgctttt ggcgcttatg ccgtgcagtt tttgcatgaa
    31741 ggcagccagg aggagcacct tattttgctc tacgcattgg ggatcattgc ggtgatgacg
    31801 cttttcaact ccttaagcaa ccatgcggta gggcggctgg aagtgatcct cgtcggcatt
    31861 aaaatgatga tcctgttatt gctgatcatt gcaggtgtct ggtcgctgca accggcgcat
    31921 atttccgtct ctgcgccccc cagctccggt gcgttcttct cctgtatcgg gataaccttc
    31981 cttgcctatg cgggctttgg catgatggcg aacgcggcgg ataaagtgaa agatccgcag
    32041 gtcattatgc cacgggcgtt tctggtggcg attggcgtta ccacgttgct ttatatctcg
    32101 ctggcgctgg ttttgcttag cgatgtgtcg gcattagagt tagaaaaata tgctgatacc
    32161 gccgtagcgc aggctgcttc tccgctgctc gggcatgtgg gttatgtgat cgtcgtcatc
    32221 ggcgctttac tggcgacggc ttcagccatt aacgcgaacc tgttcgccgt gtttaacatc
    32281 atggacaaca tgggcagcga acgcgaactg ccgaagctaa tgaataaacc tctgtggcgg
    32341 cagagtacct ggggcaacat catcgtcgtg gtgttgatta tgctgatgac ggcggcgctg
    32401 aatttaggct cactcgccag cgttgccagc gccacctttt tgatctgcta tctggcggtg
    32461 tttgtggtgg cgatccgcct gcgtcatgat attcacgcct cgttgccgat tcttatcgtt
    32521 ggtacgttgg tgatgttgtt ggtgatcgtt ggctttatct acagtctctg gtcccagggc
    32581 agccgtgcgc tgatatggat tattggcgca ctcttactca gccttattgt ggcaatggtc
    32641 atgaagcgca ataaaactgt ataacatctc tctgtgcgca gtacctcctg tattattgtg
    32701 gtggcggtcg atattcgcac tggcaaaaaa acgtgcttga atatctgttg aaacccttta
    32761 acaaagcaca ggaggcgttg cgcgaacgat gaacattagc gatgtagcaa aaattaccgg
    32821 cctgaccagc aaagccattc gcttctatga agagaagggg ctggtgacgc cgccgatgcg
    32881 cagcgaaaac ggttatcgca cctacacgca gcagcatctc aacgaactga ccttactgcg
    32941 ccaggcacgc caggtgggct ttaacctgga agagagcggc gaactggtga atctgtttaa
    33001 cgatccgcag cgtcacagcg ccgacgtcaa acgtcgtacg ctggagaaag tagcggagat
    33061 cgaacagcat atcgaagaac tgcaatccat gcgtaaccag ctgctggcgc tggcgaatgc
    33121 ctgccccggt gatgacagcg ccgactgccc gattatcgaa aatctctccg gctgctgtca
    33181 tcatcgggcg gggtgattaa ggcttgcgaa aatagtcgtc cattattttc gtcagctccg
    33241 gacattcccc gtcatcattg atggcaaaat ccagaaaaaa agcatctgct tgcggcgttc
    33301 gcttttgcgc catcaaccgg aaggtgaggg ggcctgacca ggtttcggat gggctgatgt
    33361 gcttatcagg gccgaaactc tcccatgcgc tgggcggtaa tgttttgcac gcggtgagtt
    33421 ttggtatgtg gtattgcagc tctgttttgt gcagacattc cccgccagga aataccaggg
    33481 tttgttgctc atcattaaaa tgggttgccg tgctgagcac cagtgttttg agcggtcgcc
    33541 cttccagcgc ggcggcagta gccttatggt gtccgtcgag cagaacgcag agataggaat
    33601 cagggagata ccaggccaga gcgcgggcga ttccagtttt cgagagcaga caaaaaccgg
    33661 gcggctccct gttcatcctc cgcaaaaccg ctgtagacaa attccgcaca ggtagggcaa
    33721 ccagcctcgg accagaaacg tattaacggg acatccttgc cagcagagcg aatggataag
    33781 accgtgcgag tgcggatcgt aaatcacaca ttgacgcaga aatcctttcc cggacttcac
    33841 cgaaaaaatg acgcccgcag cggtattttt cagcagacgt acttcaggtt ttcgacggga
    33901 aaaagcatgg ccactcctta gccaaaattt cagttccctg aactgtgcca gaaaattacg
    33961 ctggttacta ttgctctgga aatggaaacg gtgtccttac ccattaatcc ccgtttgtac
    34021 cgaaagaata tgctgcaaac tttctaccat aatgcgcccc gactgaaata agccttcctg
    34081 gatttcattg ttaacattca cagcgtgaac cagggaggct aataacacca cttgcaagaa
    34141 ggcaaaaata aacgcaccga taatcataat atgcgtcagg gggattttcc tttgctcctt
    34201 cattttaaat aacccacaaa ctggccttct ttcattaagg cgacttttcc tgtgtgatga
    34261 taaacaatga cattatacag acccgactga taaaccgtgg catcggcatt atcttcatct
    34321 atggcacata atgaatcatt gagataagca aagttcttac tactcattct aaaggtcatg
    34381 ccaaatcctt tataaatggc tttttttgcc gcctgggcat gttgtgcggc ttctgcattt
    34441 ttaaatgcct gcgcttcaga agcacttaaa atagccgtct ggcgctgctg ttctgcgaga
    34501 taacaggtgt catggctgac gccctgcgcc tgacaatctg cgatgcggga agcgggtgag
    34561 ctacagccag ccagtaatat aaccgccagc agggaaaagg atttaatgga gctattcatc
    34621 attgttaacc ttaaataatg tgcgaattaa ttaagaggtg aaacccggca agcttccttg
    34681 ctcttattta tattgaggat tgaaatatta gaacgaatat cctacgccga cacctgcgcc
    34741 gaaatcagat tgcgtatccg cagaaatacc ggctttaagt accgtatgcg ttccggcatt
    34801 aaaagatgtt ccgacagaca ccgcagattc accgttgaag gttcccgctc ccgctgacac
    34861 catcatcaag tcatcggttt tcacctgtgg ttgtgaggcg atagcgatgg ctgatgcagt
    34921 tcccgcattg gcttctttac ggttgtcctc aacttcgttt ttcagcgaac tgaaatggtt
    34981 attgagcgtg ttaatttgtt ggctgttatc tgcgacccgt ttttgcgtgt tgcttaactg
    35041 ttgctgcgtt tgtgcggaat cttgtctcag actttcaatt tgctcatgtg cctggacaga
    35101 cttctgcgcc agactttgca tttgtttatc gtattgagcc ctttcttttt gctgttgtgc
    35161 tgccacatcg gttgtcagtg cattcatttt ctgcccttgt tccgtggtag tgcgctgatt
    35221 agcctcattt tgagccgcca gtctggcatt aatcatctcc tgataatgcg cggtattttg
    35281 cgtatctcca gtcgcggcga gcgttttttg tgtggctacg atctgcgcct gttgctctgt
    35341 aatttgcgtt tgctgggcat cagttaccgc agactgtcgg gcgttgatca tgctctgata
    35401 acgggcaggt gtctgtgcat cacctgtagc ggcaacggtt ttttgtcctg catcaggatc
    35461 tgtctggaca gggggagtct gagtggtatc aggtgtttca gaagggatgt agtgatgttc
    35521 agtcaaatag gtatacgcag cggggctgtt gtctgtgcgt tctaaaaagc gggtaatctt
    35581 ctgctcaatc tccgattgcg acatgccaga gttaatggct gaaagctctg ttgccgccgc
    35641 ttcaatggta tcaccagcta aaacaacagg gctggatgtt gctgaaatta tgagtgccag
    35701 taaagctacg tttacagttt tca
//
LOCUS       DADSYM010000031        35389 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2415
            SAMN08039579-rid11723173.denovo.031, whole genome shotgun sequence.
ACCESSION   DADSYM010000031 DADSYM010000000
VERSION     DADSYM010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039579
            Sequence Read Archive: SRR6362533
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 35389)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35389)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980162.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 03:39:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,484
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 4,347
            CDSs (with protein)               :: 4,347
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 43 of 85
            Pseudo Genes (incomplete)         :: 37 of 85
            Pseudo Genes (internal stop)      :: 19 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35389
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039579-rid11723173.denovo.031"
                     /strain="LGE2415"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8302 N 95.9628 W"
                     /collection_date="2015-08-24"
     gene            complement(<1..469)
                     /locus_tag="J1Y83_001284"
     CDS             complement(<1..469)
                     /locus_tag="J1Y83_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003843851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HBA7629449.1"
                     /translation="MIIEKVMNNNCVQASMNGQEVIISGPGVGYNKKYGMSVPEHPAN
                     RIFYVRNEQKNKLYKLIEHVDIEYVFVAEKIVQYAEKNLEKNLNPSLLLILADHISNA
                     ISRVVSGIQINNVFLDEIKALYKAEYAISRDALTIINEQFSVQLPDDEIGFIAL"
     gene            complement(727..1980)
                     /locus_tag="J1Y83_001285"
     CDS             complement(727..1980)
                     /locus_tag="J1Y83_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMIN domain-containing protein"
                     /protein_id="HBA7629450.1"
                     /translation="MSGSNTAISRRRLLQGAGAMWLLSVSQVSLAAVSQVVAVRVWPA
                     SSYTRVTVESNRQLKYKQFALSNPERVVVDIEDVNLNSVLKGMAAQIRADDPFIKSAR
                     VGQFDPQTVRMVFELKQNVKPQLFALAPVAGFKERLVMDLYPANAQDMQDPLLALLED
                     YNKGDLEKQVPPAQSGPQPGKAGRDRPIVIMLDPGHGGEDSGAVGKYKTREKDVVLQI
                     ARRLRSLIEKEGNMKVYMTRNEDIFIPLQVRVAKAQKQRADLFVSIHADAFTSRQPSG
                     SSVFALSTKGATSTAAKYLAQTQNASDLIGGVSKSGDRYVDHTMFDMVQSLTIADSLK
                     FGKAVLNKLGKINKLHKNQVEQAGFAVLKAPDIPSILVETAFISNVEEERKLKTATFQ
                     QEVAESILAGIKAYFADGATLARRG"
     gene            2212..3543
                     /gene="argA"
                     /locus_tag="J1Y83_001286"
     CDS             2212..3543
                     /gene="argA"
                     /locus_tag="J1Y83_001286"
                     /EC_number="2.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino-acid N-acetyltransferase"
                     /protein_id="HBA7629451.1"
                     /translation="MVKERKTELVEGFRHSVPYINTHRGKTFVIMLGGEAIEHENFSS
                     IVNDIGLLHSLGIRLVVVYGARPQIDANLAAHHHEPLYHKNIRVTDAKTLELVKQAAG
                     TLQLDITARLSMSLNNTPLQGAHINVVSGNFIIAQPLGVDDGVDYCHSGRIRRIDEDA
                     IHRQLDSGAIVLMGPVAVSVTGESFNLTSEEIATQLAIKLKAEKMIGFCSSQGVTNDD
                     GDIVSELFPNEAQARVEAQEKKGDYNSGTVRFLRGAVKACRSGVRRCHLISYQEDGAL
                     LQELFSRDGIGTQIVMESAEQIRRATINDIGGILELIRPLEQQGILVRRSREQLEMEI
                     DKFTIIQRDNTTIACAALYPFPEEKIGEMACVAVHPDYRSSSRGEVLLERIAAQAKQS
                     GLSKLFVLTTRSIHWFQERGFTPVDIDLLPESKKQLYNYQRKSKVLMADLG"
     gene            complement(3625..5451)
                     /gene="recD"
                     /locus_tag="J1Y83_001287"
     CDS             complement(3625..5451)
                     /gene="recD"
                     /locus_tag="J1Y83_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000775924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit alpha"
                     /protein_id="HBA7629452.1"
                     /translation="MKLQKQLLEAVEHKQLRPLDVQFALTVAGDEHPAVTLAAALLSH
                     DAGEGHVCLPLSRLENNEASHPLLATCVSEIGELQNWEECLLASQAVSRGDEPTPMIL
                     CGDRLYLNRMWCNERTVARFFNEVNHAIEVDEALLAQTLDKLFPVSDEINWQKVAAAV
                     ALTRRISVISGGPGTGKTTTVAKLLAALIQMADGERCRIRLAAPTGKAAARLTESLGK
                     ALRQLPLTDEQKKRIPEDASTLHRLLGAQPGSQRLRHHAGNPLHLDVLVVDEASMIDL
                     PMMSRLIDALPDHARVIFLGDRDQLASVEAGAVLGDICAYANAGFTAERAGQLSRLTG
                     SHVPAGTGTEAASLRDSLCLLQKSYRFGSDSGIGQLAAAINRGDKTAVKTVFQQDFTD
                     IEKRLLQSGEDYIAMLEEALAGYGRYLDLLQARAEPDLIIQAFNEYQLLCALREGPFG
                     VAGLNERIEQFMQQKRKIHRHPHSRWYEGRPVMIARNDSALGLFNGDIGIALDRGQGT
                     RVWFAMPDGNIKSVQPSRLPEHETTWAMTVHKSQGSEFDHAALILPSQRTPVVTRELV
                     YTAVTRARRRLSLYADERILSAAIATRTERRSGLAALFSSRG"
     gene            complement(5451..8993)
                     /gene="recB"
                     /locus_tag="J1Y83_001288"
     CDS             complement(5451..8993)
                     /gene="recB"
                     /locus_tag="J1Y83_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit beta"
                     /protein_id="HBA7629453.1"
                     /translation="MSDVAETLDPLRLPLQGERLIEASAGTGKTFTIAALYLRLLLGL
                     GGSAAFPRPLTVEELLVVTFTEAATAELRGRIRSNIHELRIACLRETTDNPLYKRLLE
                     EIDDKAQAAQWLLLAERQMDEAAVFTIHGFCQRMLNLNAFESGMLFEQQLIEDESLLR
                     YQACADFWRRHCYPLPREIAQVVFETWKGPQALLRDINRYLQGEAPVIKAPPPDDETL
                     ASRHAQIVARIDAVKQQWRDAVGELDALIESSGIDRRKFNRSNQAKWIEKISAWAEEE
                     TNSYQLPESLEKFSQRFLEDRTKAGGETPRHPLFEAIEQLLAEPLSIRDLVITRALAE
                     IRETVAREKRRRGELGFDDMLSRLDSALRSESGEVLAAAIRTRFPVAMIDEFQDTDPQ
                     QYRIFRRIWHHQPETALLLIGDPKQAIYAFRGADIFTYMKARSEVHAHYTLDTNWRSA
                     PGMVNSVNKLFSQTDDAFMFREIPFIPVKSAGKNQALRFVFKGETQPAMKMWLMEGES
                     CGVGDYQSTMAQVCAAQIRDWLQAGQRGEALLMNGDDARPVRASDISVLVRSRQEAAQ
                     VRDALTLLEIPSVYLSNRDSVFETLEAQEMLWLLQAVMTPERENTLRSALATSMMGLN
                     ALDIETLNNDEHAWDAVVEEFDGYRQIWRKRGVMPMLRALMSARNIAENLLATAGGER
                     RLTDILHISELLQEAGTQLESEHALVRWLSQHILEPDSNASSQQMRLESDKHLVQIVT
                     IHKSKGLEYPLVWLPFITNFRVQDQAFYHDRHSFEAVLDLNAAPESVDLAEAERLAED
                     LRLLYVALTRSVWHCSLGVAPLVRRRGDKKGDTDVHQSALGRLLQKGEPQDAAGLRTC
                     IEALCNDDIAWQTAQIGDNQPWQVNDALTAELNARTLQRLPGDNWRVTSYSGLQQRGH
                     GIAQDLMPRLDVDAAGVVSVVEEPTLTPHQFPRGASPGTFLHSLFEDLDFTQPVDPNW
                     VQEKLELGGFEPQWEPVLTEWITTVLLAPLNETGISLSQLSDRDKQVEMEFYLPISEP
                     LIASQLDALIRQFDPLSAGCPPLEFMQVRGMLKGFIDLVFRHEGRYYLLDYKSNWLGE
                     DSSAYTQQAMAAAMQAHRYDLQYQLYTLALHRYLRHRIADYDYERHFGGVIYLFLRGV
                     DKEHPQQGIYATRPNAGLIDLMDEMFAGMTLEEA"
     gene            complement(8986..11874)
                     /gene="ptrA"
                     /locus_tag="J1Y83_001289"
     CDS             complement(8986..11874)
                     /gene="ptrA"
                     /locus_tag="J1Y83_001289"
                     /EC_number="3.4.24.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin"
                     /protein_id="HBA7629454.1"
                     /translation="MPRSIWFKALLLFVALWAPLSQAETGWQPIQETIRKSDKDNRQY
                     QAIRLDNGMVVLLVSDPQAVKSLSALVVPVGSLEDPEAYQGLAHYLEHMSLMGSKKYP
                     QADSLAEYLKMHGGSHNASTAPYRTAFYLEVENDALPGAVDRLADAIAEPLLDKKYAE
                     RERNAVNAELTMARTRDGMRMAQVSAETINPAHPGSKFSGGNLETLSDKPGNPVQQAL
                     KDFHEKYYSANLMKAVIYSNKPLPELAKMAADTFGRVPNKESKKPEITVPVVTDAQKG
                     IIIHYVPALPRKVLRVEFRIDNNSAKFRSKTDELITYLIGNRSPGTLSDWLQKQGLVE
                     GISANSDPIVNGNSGVLAISASLTDKGLANRDQVVAAIFSYLNLLREKGIDKQYFDEL
                     ANVLDIDFRYPSITRDMDYVEWLADTMIRVPVEHTLDAVNIADRYDAKAVKERLAMMT
                     PQNARIWYISPKEPHNKTAYFVDAPYQVDKISAQTFADWQKKAANIALSLPELNPYIP
                     DDFSLIKSEKKYDHPELIVDESNLRVVYAPSRYFASEPKADVSLILRNPKAMDSARNQ
                     VMFALNDYLAGLALDQLSNQASVGGISFSTNANNGLMVNANGYTQRLPQLFQALLEGY
                     FSYTATEDQLEQAKSWYNQMMDSAEKGKAFEQAIMPAQMLSQVPYFSRDERRKILPSI
                     TLKEVLAYRDTLKSGARPEFMVIGNMTEAQATTLARHVQKQLGADGSEWCRNKDVVVD
                     KKQSVIFEKAGNSTDSALAAVFVPTGYDEYTSSAYSSLLGQIVQPWFYNQLRTEEQLG
                     YAVFAFPMSVGRQWGMGFLLQSNDKQPSFLWERYKAFFPTAEAKLRAMKPEEFAQIQQ
                     AVITQMLQAPQTLGEEASKLSKDFDRGNMRFDSRDKIVAQIKLLTPQKLADFFHQAVV
                     EPQGMAILSQISGSQNGKAEYVHPEGWKVWENVSALQQTMPLMSEKNE"
     gene            complement(12050..15418)
                     /gene="recC"
                     /locus_tag="J1Y83_001290"
     CDS             complement(12050..15418)
                     /gene="recC"
                     /locus_tag="J1Y83_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit gamma"
                     /protein_id="HBA7629455.1"
                     /translation="MLRVYHSNRLDVLEALMEFIVERERLDDPFEPEMILVQSTGMAQ
                     WLQMTLSQKFGIAANIDFPLPASFIWDMFVRVLPEIPKESAFNKQSMSWKLMTLLPQL
                     LEREDFTLLRHYLTDDSDKRKLFQLSSKAADLFDQYLVYRPDWLAQWETGHLVEGLGE
                     AQAWQAPLWKALVEYTDELGQPRWHRANLYQRFIETLESATTCPPGLPSRVFICGISA
                     LPPVYLQALQALGKHIEIHLLFTNPCRYYWGDIKDPAYLAKLLTRQRRHSFEDRELPL
                     FRDSENAGQLFNSDGEQDVGNPLLASWGKLGRDYIYLLSDLESSQELDAFVDVTPDNL
                     LHNIQSDILELENRAVAGVNIEEFSRSDNKRPLDPLDSSITFHVCHSPQREVEVLHDR
                     LLAMLEEDPTLTPRDIIVMVADIDSYSPFIQAVFGSAPADRYLPYAISDRRARQSHPV
                     LEAFISLLSLPDSRFVSEDVLALLDVPVLAARFDITEEGLRYLRQWVNESGIRWGIDD
                     DNVRELELPATGQHTWRFGLTRMLLGYAMESAQGEWQSVLPYDESSGLIAELVGHLAS
                     LLMQLNIWRRGLAQERPLEEWLPVCRDMLNAFFLPDAETEAAMTLIEQQWQAIIAEGL
                     GAQYGDAVPLSLLRDELALRLDQERISQRFLAGPVNICTLMPMRSIPFKVVCLLGMND
                     GVYPRQLAPLGFDLMSQKPKRGDRSRRDDDRYLFLEALISAQQKLYISYIGRSIQDNS
                     ERFPSVLVQELIDYIGQSHYLPGDEALNCDESEARVKAHLTCHHTRMPFDPQNYQPGN
                     LQSYAREWLPAASQAGKAHSEFVQPLPFTLPETVPLETLQRFWAHPVRAFFQMRLQVN
                     FRTEDSEIPDTEPFILEGLSRYQINQQLLNVLVEQDDAERLFRRFRAAGDLPYGAFGE
                     IFWETQCQEMQQLADRVIACRQPGQSMEIDLACNGVQITGWLPQVQPDGLLRWRPSLL
                     SVAQGMQLWLEHLVYCASGGNGESRLFLRKDGEWRFPPLAAEQALHYLSQLIEGYREG
                     MSAPLLVLPESGGAWLKTCYDAQNDAMLDDDSTLQKARTKFLQAYEGNMMVRGEGDDI
                     WYQRLWRQLTPETMEAIVEQSQRFLLPLFRFNQS"
     gene            complement(15431..15754)
                     /locus_tag="J1Y83_001291"
     CDS             complement(15431..15754)
                     /locus_tag="J1Y83_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HBA7629456.1"
                     /translation="MSASLRNQQGFSLPEVMLAMVLMVMIVTALSGFQRTLMNSLASR
                     NQYQQLWRHGWQQTQLRAISPPANWQVNRMQTSQAGCVSISVTLVSPGGREGEMTRLH
                     CPNRQ"
     gene            complement(15739..16146)
                     /locus_tag="J1Y83_001292"
     CDS             complement(15739..16146)
                     /locus_tag="J1Y83_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311708.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2509 family protein"
                     /protein_id="HBA7629457.1"
                     /translation="MNREKGVSSLALVLMLLILGSLLLQGMSQQDRSFASRVSMESQS
                     LRRQAIVQSALEWGKMHSWQTLPAVQCLLYAATGARVCLRLLADNEALLIAGYEGVSL
                     WRTGEVIDGNIVFSPRGWSDFCPLKERALCQLP"
     gene            complement(16143..16706)
                     /locus_tag="J1Y83_001293"
     CDS             complement(16143..16706)
                     /locus_tag="J1Y83_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent protein"
                     /protein_id="HBA7629458.1"
                     /translation="MPVKEQGFSLLEVLIAMAISSVLLLGAARFLPALQRESLTNTRK
                     LALEDEIWLRVFTVAKHLQRAGYCHGSCTGEGLEIVGQGDCVIVQWDANSNGIWDREP
                     VKESDQIGFRLKEHVLETLRGATSCEGKGWDKVTNPDAIIIDTFQVVRQDVSGFSPVL
                     TVNMHAASKADPQTVVDASYSVTGSNL"
     gene            complement(16697..17167)
                     /locus_tag="J1Y83_001294"
     CDS             complement(16697..17167)
                     /locus_tag="J1Y83_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent protein"
                     /protein_id="HBA7629459.1"
                     /translation="MKTQRGYTLIETLVAMLILVMLSASGLYGWQYWQQSQRLWQTAS
                     QARDYLLYLREDANWHNRDHSISLIREGTLWCLVSSVAGANTCHGSSPLVFMPRWPEV
                     EMSDLTPSLAFFGLRNTAWAGHIRFKNSTGEWWLVVSPWGRLRLCQQGETEGCL"
     gene            complement(17352..18146)
                     /gene="thyA"
                     /locus_tag="J1Y83_001295"
     CDS             complement(17352..18146)
                     /gene="thyA"
                     /locus_tag="J1Y83_001295"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HBA7629460.1"
                     /translation="MKQYLELMQKVLDEGTQKNDRTGTGTLSIFGHQMRFNLQDGFPL
                     VTTKRCHLRSIIHELLWFLQGDTNIAYLHENNVTIWDEWADENGDLGPVYGKQWRAWP
                     TPDGRHIDQITTVLNQLKNDPDSRRIIVSAWNVGELDKMALAPCHAFFQFYVADGKLS
                     CQLYQRSCDVFLGLPFNIASYALLVHMMAQQCDLEVGDFVWTGGDTHLYSNHMDQTHL
                     QLSREPRPLPKLIIKRKPESIFDYRFEDFEIEGYDPHPGIKAPVAI"
     gene            complement(18153..19028)
                     /gene="lgt"
                     /locus_tag="J1Y83_001296"
     CDS             complement(18153..19028)
                     /gene="lgt"
                     /locus_tag="J1Y83_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HBA7629461.1"
                     /translation="MTSSYLHFPEFDPVIFSIGPVALHWYGLMYLVGFIFAMWLATRR
                     ANRPGSGWTKNEVENLLYAGFLGVFLGGRIGYVLFYNFPQFMADPLYLFRVWDGGMSF
                     HGGLIGVIVVMIIFARRTKRSFFQVSDFIAPLIPFGLGAGRLGNFINGELWGRVDPNF
                     PFAMLFPGSRTEDILLLQTNPQWQSIFDTYGVLPRHPSQLYELLLEGVVLFIILNLYI
                     RKPRPMGAVSGLFLIGYGAFRIIVEFFRQPDAQFTGAWVQYISMGQILSIPMIVAGVI
                     MMVWAYRRSPQQHVS"
     gene            complement(19179..21425)
                     /gene="ptsP"
                     /locus_tag="J1Y83_001297"
     CDS             complement(19179..21425)
                     /gene="ptsP"
                     /locus_tag="J1Y83_001297"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HBA7629462.1"
                     /translation="MLTRLREIVEKVASAPRLNEALNILVTDICLAMDTEVCSVYLAD
                     HDRRCYYLMATRGLKKPRGRTVTLAFDEGIVGLVGRLAEPINLADAQKHPSFKYIPSV
                     KEERFRAFLGVPIIQRRQLLGVLVVQQRELRQYDESEESFLVTLATQMAAILSQSQLT
                     ALFGQYRQTRIRALPAAPGVAIAEGWQDATLPLMEQVYQASTLDPALERERLTGALEE
                     AANEFRRYSKRFAAGAQKETAAIFDLYSHLLSDTRLRRELFAEVDKGSVAEWAVKTVI
                     EKFAEQFAALSDNYLKERAGDLRALGQRLLFHLDDANQGPNAWPERFILVADELSATT
                     LAELPQDRLVGVVVRDGAANSHAAIMVRALGIPTVMGADIQPSVLHRRTLIVDGYRGE
                     LLVDPEPVLLQEYQRLISEEIELSRLAEDDVNLPAQLKSGERIKVMLNAGLSPEHEEK
                     LGSRIDGIGLYRTEIPFMLQSGFPSEEEQVAQYQGMLQMFNDKPVTLRTLDVGADKQL
                     PYMPISEENPCLGWRGIRITLDQPEIFLIQVRAMLRANAATGNLNILLPMVTSLDEVD
                     EARRLIERAGREVEEMIGYEIPKPRIGIMLEVPSMVFMLPHLAKRVDFISVGTNDLTQ
                     YILAVDRNNTRVANIYDSLHPAMLRALAMIAREAEIHGIDLRLCGEMAGDPMCVAILI
                     GLGYRHLSMNGRSVARVKYLLRRIDFAEAENLAQRSLEAQLATEVRHQVAAFMERRGM
                     GGLIRGGL"
     gene            complement(21438..21968)
                     /gene="rppH"
                     /locus_tag="J1Y83_001298"
     CDS             complement(21438..21968)
                     /gene="rppH"
                     /locus_tag="J1Y83_001298"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001655775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA pyrophosphohydrolase"
                     /protein_id="HBA7629463.1"
                     /translation="MIDDDGYRPNVGIVICNRQGQVMWARRFGQHSWQFPQGGINPGE
                     SAEQAMYRELFEEVGLSRKDVRILASTRNWLRYKLPKRLVRWDTKPVCIGQKQKWFLL
                     QLVSGDAEINMQTSSTPEFDGWRWVSYWYPVRQVVSFKRDVYRRVMKEFASVVMSLQE
                     NTPKPQNASAYRRKRG"
     gene            22653..23342
                     /gene="mutH"
                     /locus_tag="J1Y83_001299"
     CDS             22653..23342
                     /gene="mutH"
                     /locus_tag="J1Y83_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutH"
                     /protein_id="HBA7629464.1"
                     /translation="MSQPRPLLSPPETEEQLLAQAQQLSGYTLGELAALAGLVTPENL
                     KRDKGWIGVLLEIWLGASAGSKPEQDFAALGVELKTIPVDSLGRPLETTFVCVAPLTG
                     NSGVTWETSHVRHKLKRVLWIPVEGERSIPLAKRRVGSPLLWSPNEEEDRQLREDWEE
                     LMDMIVLGQVERITARHGEYLQIRPKAANAKALTEAIGARGERILTLPRGFYLKKNFT
                     SALLARHFLIQ"
     gene            23411..24124
                     /locus_tag="J1Y83_001300"
     CDS             23411..24124
                     /locus_tag="J1Y83_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HBA7629465.1"
                     /translation="MLFAWITDPNAWLALGTLTLLEIVLGIDNIIFLSLVVAKLPTAQ
                     RAHARRLGLAGAMVMRLALLASIAWVTRLTNPLFTIFSQEISARDLILLLGGLFLIWK
                     ASKEIHESIEGEEEGLKTRVSSFLGAIVQIMLLDIIFSLDSVITAVGLSDHLFIMMAA
                     VVIAVGVMMFAARSIGDFVERHPSVKMLALSFLILVGFTLILESFDIHVPKGYIYFAM
                     FFSIAVESLNLIRNKKNPL"
     gene            24262..24480
                     /gene="ygdR"
                     /locus_tag="J1Y83_001301"
     CDS             24262..24480
                     /gene="ygdR"
                     /locus_tag="J1Y83_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YgdR"
                     /protein_id="HBA7629466.1"
                     /translation="MKKWAVIISAVGLAFAVSGCSSDYVMATKDGRMILTDGKPEIDD
                     DTGLVSYHDQQGNAMQINRDDVSQIIER"
     gene            24588..25628
                     /locus_tag="J1Y83_001302"
     CDS             24588..25628
                     /locus_tag="J1Y83_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(H)-dependent aldo-keto reductase"
                     /protein_id="HBA7629467.1"
                     /translation="MQYHRIPHSSLEVSTLGLGTMTFGEQNSEADAHAQLDYAVAQGI
                     NLIDVAEMYPVPPRPETQGLTETYVGNWLAKHGSREKLIIASKVSGPSRNNDKGIRPD
                     QALDRKNIREALHDSLKRLQTDYLDLYQVHWPQRPTNCFGKLGYSWTDSAPAVSLLDT
                     LDALAEYQRAGKIRYIGVSNETAFGVMRYLHLADKHDLPRIVTIQNPYSLLNRSFEVG
                     LAEVSQYEGVELLAYSCLGFGTLTGKYLNGAKPAGARNTLFSRFTRYSGEQTQKAVAA
                     YVDIARRHGLDPAQMALAFVRRQPFVASTLLGATTMEQLKTNVESLHLELSEDILAEI
                     EAVHQVYTYPAP"
     gene            complement(25660..26853)
                     /gene="lplT"
                     /locus_tag="J1Y83_001303"
     CDS             complement(25660..26853)
                     /gene="lplT"
                     /locus_tag="J1Y83_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid transporter LplT"
                     /protein_id="HBA7629468.1"
                     /translation="MSESVHTNTSLWSKGMKAVIVAQFLSAFGDNALLFATLALLKAQ
                     FYPEWSQPILQMVFVGAYILFAPFVGQVADSFAKGRVMMFANGLKLLGAASICFGINP
                     FLGYTLVGVGAAAYSPAKYGILGELTTGSKLVKANGLMEASTIAAILLGSVAGGVLAD
                     WHVLVALAACALAYGGAVVANIYIPKLAAARPGQSWNLINMTRSFLNACTSLWRNGET
                     RFSLVGTSLFWGAGVTLRFLLVLWVPVALGITDNATPTYLNAMVAIGIVVGAGAAAKL
                     VTLETVSRCMPAGILIGVVVLIFSLQHELLPAYALLMLIGVLGGFFVVPLNALLQERG
                     KKSVGAGNAIAVQNLGENSAMLLMLGIYSLAVMVGIPVVPIGIGFGALFALAITALWI
                     WQRRH"
     gene            complement(26846..29005)
                     /gene="aas"
                     /locus_tag="J1Y83_001304"
     CDS             complement(26846..29005)
                     /gene="aas"
                     /locus_tag="J1Y83_001304"
                     /EC_number="2.3.1.40"
                     /EC_number="6.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acyl-ACP--phospholipid
                     O-acyltransferase/long-chain-fatty-acid--ACP ligase"
                     /protein_id="HBA7629469.1"
                     /translation="MLFSFFRNLCRVLYRVRVTGDTQALKGERVLITPNHVSFIDGIL
                     LALFLPVRPVFAVYTSISQQWYMRWLKSFIDFVPLDPTQPMAIKHLVRLVEQGRPVVI
                     FPEGRITTTGSLMKIYDGAGFVAAKSGATVIPVRIEGAELTHFSRLKGLVKRRLFPQI
                     TLHILPPTQVEMPDAPRARDRRKIAGEMLHQIMMEARMAVRPRETLYESLLSAMYRFG
                     AGKKCVEDVNFTPDSYRKLLTKTLFVGRILEKYSVEGERIGLMLPNAGISAAVIFGAI
                     ARRRIPAMMNYTAGVKGLTSAITAAEIKTIFTSRQFLDKGKLWHLPEQLTQVRWVYLE
                     DLKADVNTADKVWIFAHLLMPRLAQVKQQPEEEALILFTSGSEGHPKGVVHSHKSILA
                     NVEQIKTIADFTTNDRFMSALPLFHSFGLTVGLFTPLLTGAEVFLYPSPLHYRIVPEL
                     VYDRSCTVLFGTSTFLGHYARFANPYDFYRLRYVVAGAEKLQESTKQLWQDKFGLRIL
                     EGYGVTECAPVVSINVPMAAKPGTVGRILPGMDARLLAVPGIEEGGRLQLKGPNIMNG
                     YLRVEKPGVLEVPTAENIRGEMERDWYDTGDIVRFDEQGFVQIQGRAKRFAKIAGEMV
                     SLEMVEQLALGVSPDKVHATAIKSDASKGEALVLFTTDNELMRDKLQQYAREHGVPEL
                     AVPRDIRYLKQMPLLGSGKPDFVTLKSWVDEAEQHDE"
     gene            29591..30622
                     /gene="galR"
                     /locus_tag="J1Y83_001305"
     CDS             29591..30622
                     /gene="galR"
                     /locus_tag="J1Y83_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator GalR"
                     /protein_id="HBA7629470.1"
                     /translation="MATIKDVARLAGVSVATVSRVINNSPKASEASRLAVHSAMESLS
                     YHPNANARALAQQTTETIGLVVGDVSDPFFGAMVKAVEQVAYHTGNFLLIGNGYHNEQ
                     KERQAIEQLIRHRCAALVVHAKMIPDADLASLMKQMPGMVLINRILPGFENRCIALDD
                     RYGAWLATRHLIQQGHTRIGYLCSNHSISDAEDRLQGYYDALAESGIPANDRLVTFGE
                     PDESGGEQAMTELLGRGRNFTAVACYNDSMAAGAMGVLNDNGIDVPGEISLIGFDDVL
                     VSRYVRPRLTTVRYPIVTMATQAAELALALADNRLLPEITNVFSPTLVRRHSVSTPSL
                     EASHHATSD"
     gene            complement(30629..31891)
                     /gene="lysA"
                     /locus_tag="J1Y83_001306"
     CDS             complement(30629..31891)
                     /gene="lysA"
                     /locus_tag="J1Y83_001306"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HBA7629471.1"
                     /translation="MPHSLFSTDTDLTAENLLRLPAEFGCPVWVYDAQIIRRQIAALK
                     QFDVVRFAQKACSNIHILRLMREQGVKVDSVSLGEIERALAAGYNPQTHPDDIVFTAD
                     VIDQATLERVSELQIPVNAGSVDMLDQLGQVSPGHRVWLRVNPGFGHGHSQKTNTGGE
                     NSKHGIWYTDLPAALDVIQRHHLQLVGIHMHIGSGVDYAHLEQVCGAMVRQVLEFGQD
                     LQAISAGGGLSIPYQQGEEAVDTEHYYGLWNAAREQIARHLGHPVKLEIEPGRFLVAQ
                     AGVLITQVRSVKQMGSRHFVLVDAGFNDLMRPAMYGSYHHISALAAEGRSLEHAPTVE
                     TVVAGPLCESGDVFTQQEGGNVETRALPEVKAGDYLVLHDTGAYGASMSSNYNSRPLL
                     PEVLFDNGQARLIRRRQTIEELLALELL"
     gene            32013..32948
                     /gene="lysR"
                     /locus_tag="J1Y83_001307"
     CDS             32013..32948
                     /gene="lysR"
                     /locus_tag="J1Y83_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator LysR"
                     /protein_id="HBA7629472.1"
                     /translation="MAAVNLRHIEIFHAVMTAGSLTEAAHLLHTSQPTVSRELARFEK
                     VIGLKLFERIRGRLHPTVQGLRLFEEVQRSWYGLDRIVSAAESLREFRQGELSIACLP
                     VFSQSFLPQLLQPFLARYPDVSLNIVPQESPLLEEWLSAQRHDLGLTETLHTPAGTER
                     TELLSLDEVCVLPPGHPLAVKKVLTPDDFHGENYISLSRIDSYRQLLDQLFTENQVKR
                     RMIVETHSAASVCAMVRAGVGVSVVNPLTALDYAASGLVVRRFSIAVPFTVSLIRPLH
                     RPSSALVQEFSEHLQAGLPKLVTSLDAILSSATTA"
     gene            complement(32935..33627)
                     /gene="ygeA"
                     /locus_tag="J1Y83_001308"
     CDS             complement(32935..33627)
                     /gene="ygeA"
                     /locus_tag="J1Y83_001308"
                     /EC_number="5.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid racemase"
                     /protein_id="HBA7629473.1"
                     /translation="MKTIGLLGGMSWESTIPYYRLINEGIKQRLGGLHSAQVLLHSVD
                     FHEIEECQRRGEWDKTGDILAEAALGLQRAGAEGIVLCTNTMHKVADAIESRCSLPFL
                     HIADATGRAITGAGMTRVALLGTRYTMEQDFYRGRLTEQFSINCLIPEADERAKINQI
                     IFEELCLGQFTEASRAYYAQVIARLAEQGAQGVIFGCTEIGLLVPEERSVLPVFDTAA
                     IHAEDAVAFMLS"
     gene            complement(33756..35174)
                     /locus_tag="J1Y83_001309"
     CDS             complement(33756..35174)
                     /locus_tag="J1Y83_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar porter family MFS transporter"
                     /protein_id="HBA7629474.1"
                     /translation="MVTINTESALTPRPLRDTRRMNMFVSVAAAVAGLLFGLDIGVIA
                     GALPFITDHFVLTSRLQEWVVSSMMLGAAIGALFNGWLSFRLGRKYSLMAGAILFVLG
                     SIGSAFATSVEMLIAARVVLGIAVGIASYTAPLYLSEMASENVRGKMISMYQLMVTLG
                     IVLAFLSDTAFSYSGNWRAMLGVLALPAVLLIILVVFLPNSPRWLAEKGRHIEAEEVL
                     RMLRDTSEKAREELNEIRESLKLKQGGWALFKINRNVRRAVFLGMLLQAMQQFTGMNI
                     IMYYAPRIFKMAGFTTTEQQMIATLVVGLTFMFATFIAVFTVDKAGRKPALKIGFSVM
                     ALGTLVLGYCLMQFDNGTASSGLSWLSVGMTMMCIAGYAMSAAPVVWILCSEIQPLKC
                     RDFGITCSTTTNWVSNMIIGATFLTLLDSIGAAGTFWLYTALNIAFVGITFWLIPETK
                     NVTLEHIERKLMAGEKLRNIGV"
BASE COUNT         8752 a         9644 c         8687 g         8306 t
ORIGIN      
        1 gcaatgctat aaaaccaatc tcatcatcag gaagttgaac gctgaattgc tcattaatga
       61 tagttaatgc atcgcgactt atcgcatact ctgctttgta caacgctttg atttcatcaa
      121 ggaaaacatt attaatttgt atacctgaaa cgactcggga tattgcattc gaaatgtgat
      181 ccgcaagaat caatagtagc gatggattga gatttttttc gagatttttc tccgcatatt
      241 gcactatttt ttcggcaaca aacacatact caatatctac atgttcaatc aatttataaa
      301 gtttgttttt ttgttcattt ctgacataaa aaatccggtt agccggatgc tcagggaccg
      361 acattccata ttttttgttg taaccgacgc caggcccaga aatgataacc tcctgtccat
      421 tcatcgatgc ctgtacacaa ttattgttca tgactttttc gatgatcatt ccatggctcc
      481 aaaaaaaaag gcaaggcacg tcagaagtaa taactacttc aaacattgcc ttgcctgatt
      541 taacagttac aagatgatta actaatgcgt atacattaca cggaattgtg cttaattaaa
      601 gagatggtcg gcttattttt caagccgaca ttcaaatatc cgacatcagt cacaaaacca
      661 gcgtctaata ttcaggtaaa tgttatttat tcaacgaaat ataaattcat tgaataaata
      721 aaaacattat ccccttctcg ccagcgtcgc cccatcggca aaatacgctt taattcccgc
      781 aagaatagac tccgcaactt cctgctggaa agtcgccgtt ttcagtttac gctcttcctc
      841 aacgttactg ataaacgccg tttcgaccag aatggaggga atatctggtg cctttagtac
      901 ggcaaacccg gcctgttcaa cttgattttt atgcagcttg ttgattttac cgagcttatt
      961 cagcaccgct ttaccaaact taaggctgtc ggcaatggtc agcgactgca ccatatcgaa
     1021 catggtgtgg tcgacatagc ggtcaccgct tttgcttacg ccaccaatca agtccgaggc
     1081 gttctgggtt tgtgccagat attttgccgc agtactggtt gcgcctttgg ttgagagcgc
     1141 aaacaccgat gaaccgctcg gctggcgact ggtaaaggcg tcggcatgga tagaaacaaa
     1201 caagtcagcg cgctgtttct gggcttttgc tacgcgcact tgcaacggaa tgaagatgtc
     1261 ttcattgcgc gtcatgtaca ccttcatatt gccctctttc tcgatcagag agcgcagacg
     1321 gcgagctatt tgcaatacca cgtctttttc gcgcgttttg tatttcccca ccgcaccgga
     1381 gtcttcgcca ccgtggccag ggtcaagcat aatgacaatc ggacgatccc gcccagcttt
     1441 acccggttgt ggaccacttt gtgctggcgg cacctgcttt tcgaggtcgc ctttgttgta
     1501 atcctccagc agcgccagca gcgggtcctg catatcctgt gcattggccg gatagaggtc
     1561 catcaccaga cgctctttaa acccggcgac cggcgcaagg gcaaacagct gcggttttac
     1621 gttttgcttt aattcaaaaa ccatacgtac ggtttgcggg tcaaattgcc cgacgcgcgc
     1681 cgacttgatg aacgggtcgt cagcgcggat ttgcgcagcc atccccttga gcaccgagtt
     1741 caggtttaca tcttcgatat ccaccaccac gcgttcagga ttactcaacg cgaactgctt
     1801 atatttcagc tgacgatttg attctaccgt cacgcgggtg tagctggacg caggccagac
     1861 gcgcaccgcc acgacctggc tgaccgcagc caggctgacc tgacttacgc tcaatagcca
     1921 catggcaccc gcgccttgca gtaaacgacg acggctgatt gcagtgttgg atcctgacat
     1981 gcctctcccg agcaaaagaa atctaagctg tgtaacaagt aaacgaccaa tttgaccgga
     2041 aactttagcg aaagacgcat aatctgtcat ctataaaacg gtaaacattc tttttatatt
     2101 cacggtatta ctgataaaaa actcgctccc gcataaaatt tacacttgca ccctgcgaaa
     2161 aaacagaata aaaatacaat aatttcgaat aatcatgcaa agaggtatac cgtggtaaag
     2221 gaacgtaaaa ccgagctggt cgagggattc cgccattcgg ttccctatat caatacccac
     2281 cggggaaaaa cgtttgtcat catgctcggc ggtgaagcca ttgagcatga gaatttctcc
     2341 agtatcgtta atgatatcgg gttgttgcac agcctcggca tccgtctggt ggtggtctat
     2401 ggcgcacgtc cgcagatcga cgcaaatctg gctgcacatc accacgaacc gctgtatcac
     2461 aagaatatac gtgtgaccga cgccaaaaca ctggaactgg tgaagcaggc agcgggaaca
     2521 ttgcaactgg atattactgc tcgcctgtcg atgagtctca ataacacacc gctgcagggc
     2581 gcgcatatca acgtcgtcag tggtaatttt attattgccc agccgctggg cgtcgatgac
     2641 ggcgtggatt actgtcatag cgggcgtatc cggcggattg atgaagacgc gatccatcgt
     2701 caactggaca gcggtgcaat agtgctaatg gggccggtcg ctgtttcggt cactggcgag
     2761 agctttaatc tgacctcgga agagattgcc actcaactgg ccatcaaact gaaagctgaa
     2821 aaaatgattg gtttttgctc ttcccagggc gtcactaatg acgacggtga tattgtctcc
     2881 gaacttttcc ctaacgaagc gcaagcacgg gtagaagccc aggaaaagaa aggcgattac
     2941 aactccggta cggtgcgctt tttgcgtggc gcagtgaaag cctgccgcag cggcgtgcgt
     3001 cgctgtcatt taatcagtta tcaggaagat ggcgcgctgt tgcaagagtt gttctcacgc
     3061 gacggtatcg gtacgcagat tgtgatggaa agcgccgagc agattcgtcg cgcaacaatc
     3121 aacgatattg gcggtattct ggagttgatt cgcccgctgg agcaacaagg tattctggta
     3181 cgccgttctc gcgagcagct ggagatggaa atcgacaaat tcaccattat tcagcgcgat
     3241 aacacgacta ttgcctgcgc cgcgctctat ccgttcccgg aagagaagat tggggaaatg
     3301 gcctgtgtgg cagttcaccc ggattaccgc agttcatcac ggggcgaggt tctgctggaa
     3361 cgcattgccg ctcaggcgaa gcagagcggc ttaagcaaat tgtttgtgct gaccacgcgc
     3421 agtattcact ggttccagga acgtggattt accccagtgg atattgattt actgcccgag
     3481 agcaaaaagc agttgtacaa ctaccagcgt aaatccaaag tgttgatggc ggatttgggg
     3541 taagcgatgg aaatcgccgg atgcgacatg cataacattc ataggcatga taagacccac
     3601 atgcgtcgca tcaggcaatt acgtttatcc ccgtgaacta aacaacgccg ccagaccact
     3661 gcgccgctcg gtgcgcgtgg caattgccgc acttaaaatg cgctcatcgg catacagcga
     3721 cagacggcga cgcgcgcggg tcaccgcggt gtaaaccagc tctcgcgtta ctaccggcgt
     3781 gcgttgactc ggtaaaatca acgccgcatg gtcgaactcc gatccctgcg atttatgtac
     3841 cgtcatcgcc cacgtcgttt cgtgctctgg caagcgactc ggttgcacag acttaatatt
     3901 gccgtccggc atcgcaaacc agacgcgcgt cccctgcccg cgatccagcg caataccaat
     3961 atcgccatta aacaacccaa gcgcgctgtc attacgggca atcatcaccg gtcgaccttc
     4021 gtaccaacga gagtgcggat gacgatgaat tttgcgcttc tgttgcataa actgctcaat
     4081 tcgctcattc agtccagcca cgccaaacgg cccttcccgc agggcgcaca aaagctggta
     4141 ctcattgaac gcctgaatga ttaaatccgg ctcggcacgc gcttgcagca gatccagata
     4201 acgtccgtaa cccgcaagag cttcctcaag catcgcaata taatcttcgc cgctctgtaa
     4261 aagtcgtttt tcgatatcag taaaatcctg ctgaaaaacg gttttcactg ccgttttatc
     4321 accacggtta atcgccgcag ctaactgacc aatgccagaa tcgctgccga aacgatagct
     4381 tttttgcagc aggcagaggc tgtcgcgcaa agatgccgct tctgtgccag ttcctgccgg
     4441 aacgtggctt cccgtcaggc ggctcaactg cccggcacgc tcggcggtaa agccggcgtt
     4501 ggcataagcg cagatatcgc ccaacactgc cccagcctca accgaggcca gttgatcacg
     4561 atcgccgaga aagatcactc gcgcatgatc gggcaaggcg tcgatcagtc tcgacatcat
     4621 aggcagatcg atcattgacg cttcatctac caccagcaca tcaagatgca gcgggttacc
     4681 ggcatgatga cgtaaacgct ggctacccgg ctgcgcaccc agcaatcggt gcaaagtgct
     4741 ggcatcttcc ggaatgcgtt tcttttgttc atcggtcagc ggtaattgtc gcaaagcctt
     4801 gccaagagat tcggttaagc gcgcggcagc tttacccgtt ggtgcagcca gacggatacg
     4861 gcagcgttct ccgtcggcca tttgaattaa ggctgccagc aacttcgcta cggtggtcgt
     4921 tttaccggtg ccaggaccgc cggaaatcac cgaaatccgc cgcgtcagcg ccactgccgc
     4981 cgcaactttt tgccagttaa tttcatcgct tactggaaaa agtttgtcca gggtttgcgc
     5041 cagtagagct tcatcaacct caatggcatg attcacttcg ttgaaaaagc gtgccactgt
     5101 gcgctcgtta caccacatgc gattcaaata aagacgatcg ccacagagga tcatcggcgt
     5161 tggttcatct ccccggctga ccgcttgtga agccagcaag cattcttccc aattttgtag
     5221 ctcaccgatt tcactgacac aggtcgccaa cagcggatgc gatgcctcgt tattttccag
     5281 tcgtgaaagc ggcaaacaaa cgtgtccctc tccggcatca tgacttaata gtgccgccgc
     5341 gagggtgacg gcaggatgtt catctcccgc cacggtcagg gcaaactgca cgtccagcgg
     5401 gcgtagctgt ttgtgctcca cagcttccag taattgcttt tgcaatttca ttacgcctcc
     5461 tccagggtca taccggcaaa catctcatcc atcaggtcaa tcaacccggc gttgggtcgg
     5521 gtcgcgtaga tcccttgttg cggatgttct ttatcaacgc cgcgcaggaa cagataaata
     5581 acgccgccaa agtggcgctc atagtcgtaa tcagcaatac gatggcgcag ataacggtgc
     5641 agcgccaggg tataaagctg atattgcaga tcatagcggt gtgcctgcat tgccgctgcc
     5701 atagcctgtt gggtgtaagc cgaactgtct tcacccaacc agttggattt atagtcgagc
     5761 aggtaataac gcccttcgtg gcggaacacc aggtcaataa agccttttaa catgccacgt
     5821 acctgcatga actccagcgg cgggcagcca gcggatagcg ggtcaaactg gcgaattagt
     5881 gcatcaagct ggctggcgat gagcggttcg ctaattggca ggtaaaactc catctccacc
     5941 tgtttatcgc ggtcggaaag ctgactcagg ctaatacccg tttcattgag aggtgccagg
     6001 aggacagttg tgatccactc agtcaacacc ggttcccact gcggttcaaa gccaccgagc
     6061 tccagttttt cctgtaccca gttcgggtca accggctggg taaaatcgag gtcttcaaac
     6121 aaactgtgca agaatgtccc cggtgacgca ccgcgcggga actgatgcgg tgttaacgtc
     6181 ggttcttcaa cgacgctgac cacgcctgcg gcatcaacat ccagccgtgg catcagatcc
     6241 tgagcgatac cgtgaccacg ctgctgcaaa ccggagtagc tggtgacacg ccagttatcg
     6301 ccgggcaatc gttgtagcgt cctcgcattc agttctgcag ttaatgcatc attaacctgc
     6361 cagggctggt tatcaccaat ttgtgccgtt tgccaggcaa tatcatcatt gcacaacgct
     6421 tcaatacagg tacgaagccc tgccgcatct tgcggttctc ctttttgcag caaacgcccg
     6481 agcgcacttt ggtggacgtc ggtatcacct tttttatcgc cacgacggcg caccagcggt
     6541 gcaacgccga gactgcaatg ccaaaccgaa cgcgtcagcg ccacgtaaag taaacgcaga
     6601 tcttccgcca gacgttcggc ctccgcgagg tcgacgcttt ctggcgctgc attaagatcc
     6661 agaacggcct caaacgagtg gcgatcgtga taaaacgcct gatcctggac gcggaaattg
     6721 gtaataaatg gcaaccagac cagcggatat tccagccctt ttgatttgtg tatcgtgaca
     6781 atctgcacca gatgtttatc actttcgagg cgcatttgtt ggctggaggc attactgtct
     6841 ggctcgagga tatgttgcga taaccagcgt accagcgcat gttcactttc cagctgcgtt
     6901 ccggcttctt gtagcagttc gctgatatgc aagatatcgg taagacgccg ctcaccgcct
     6961 gccgttgcca gcaagttttc tgcaatgtta cgcgccgaca tcagcgcccg cagcatcggc
     7021 ataacgccac gtttgcgcca gatttgccga taaccatcga actcttcgac gaccgcatcc
     7081 cacgcatgtt cgtcattatt cagcgtttca atatccagcg cattcagccc catcattgac
     7141 gttgccagcg cactgcgcag ggtgttctct cgttcgggtg tcatcaccgc ctgcaacagc
     7201 caaagcatct cctgtgcttc cagagtttca aaaacactgt cgcggttcga aaggtaaacg
     7261 gaagggattt ccagcagcgt taaggcatcg cgcacctgag cggcctcctg gcggctgcgc
     7321 accagcacac tgatgtccga agcacgcacc ggacgcgcat cgtcgccgtt catcagcaac
     7381 gcttcgcccc gctgtccggc ttgtagccag tcgcggattt gcgcagcaca tacctgcgcc
     7441 atggtacttt gatagtcgcc aacgccgcag ctttcgcctt ccatcagcca cattttcatc
     7501 gcaggctgcg tttcaccttt aaacacaaaa cgtaacgcct gatttttccc ggctgatttc
     7561 acaggaataa acggtatttc gcgaaacatg aacgcgtcat cagtctggct gaaaagcttg
     7621 ttcacactat tcaccattcc cggtgcggaa cgccagttgg tgtctaaagt gtagtgggcg
     7681 tgaacttcgc tacgcgcctt catataagtg aagatatccg caccccggaa tgcatatatg
     7741 gcctgcttcg ggtcgccaat cagcaacaat gcggtttccg gctgatggtg ccagatacgg
     7801 cgaaaaattc ggtactgctg ggggtcggta tcctgaaatt catcgatcat tgccaccggg
     7861 aatcgcgtac ggatcgccgc agccaacacc tcgccgcttt cgctacgcag cgcggaatcg
     7921 agccgactta acatgtcatc aaaacccaat tcgccacggc ggcgtttttc acgcgctact
     7981 gtttcgcgga tctcagccaa tgcgcgggtg atcaccagat cgcggatcga caatggttct
     8041 gcaagcagtt gttcgatcgc ctcaaacagt ggatgtcgcg gggtttcccc cccggctttc
     8101 gtgcgatctt ctaagaaacg ctgagagaat ttttccagcg actccggcaa ctgatagctg
     8161 ttggtctctt cttctgccca ggcgctgatc ttctcgatcc atttagcctg attgctacgg
     8221 ttaaacttgc gtcgatcaat accagaagat tcgatcagcg catccagttc acccactgcg
     8281 tcgcgccact gctgttttac ggcatcaata cgcgccacaa tttgcgcgtg gcgggaagcc
     8341 agcgtttcat catcgggggg cggtgctttg ataaccggcg cttcgccttg cagataacga
     8401 ttaatatcgc gcagcaacgc ctgcggccct ttccaggttt caaagacgac ctgggcaatt
     8461 tcacgcggca gcgggtagca gtggcgacgc cagaaatcgg cgcaggcctg gtagcgtagc
     8521 agagactcat cttcaatcag ctgctgctca aacagcatgc cggattcaaa ggcattcagg
     8581 ttgagcatgc gctggcaaaa gccgtgaata gtaaagactg ccgcttcatc catctgccgc
     8641 tcggctaaca acaaccactg cgcggcttgc gctttatcgt cgatctcttc cagcaggcgt
     8701 ttgtacagtg gattgtcggt ggtttcacgc agacaggcga tgcgcaactc gtggatattg
     8761 ctacggatac gaccacgcaa ttctgccgtg gcagcctcgg taaaggtcac caccagcagt
     8821 tcttcaacgg tcagcgggcg gggaaaggcg gcagaaccgc ctagtccaag taacaggcgc
     8881 aaatagagcg ccgcaatcgt aaaggttttg cctgtgccgg cagaggcttc aatcaggcgc
     8941 tcgccctgta agggcaagcg caaaggatct agtgtctcgg cgacatcact cattcttttc
     9001 actcatcagg ggcattgttt gctgcaacgc gctgacgttc tcccacactt tccagccttc
     9061 aggatgcaca tattcggctt tcccgttctg gctgccggaa atctgcgaca gaatagccat
     9121 accttgcggc tcgaccaccg cctgatggaa gaaatcagca agtttttgcg gcgtcagcag
     9181 ttttatctgg gccacgattt tatcacgcga atcgaagcgc atattgccgc gatcgaaatc
     9241 tttacttaac ttcgatgctt cttcgccgag cgtttgcggt gcctgcagca tctgagtaat
     9301 taccgcctgc tggatttgcg caaactcctc tggcttcatc gcccgcaatt ttgcctctgc
     9361 ggttgggaaa aacgccttgt aacgctccca caagaatgaa ggctgtttat cattgctttg
     9421 caaaaggaag cccatgcccc actgacgccc cacgctcatt ggaaacgcaa acacggcata
     9481 gcccaactgt tcttcggtac gcaactgatt gtagaaccac ggctgtacga tctgccccaa
     9541 cagagaacta tacgctgagc tggtgtattc atcgtagcca gtcggtacaa ataccgctgc
     9601 cagtgcggag tcggtgctgt taccagcttt ttcaaagatg acggattgtt ttttatcgac
     9661 cacgacatct ttgttacgac accactctga accatcagcg cccaactgtt tttgcacatg
     9721 gcgtgccagc gttgttgcct gggcttcagt catgttgccg ataaccataa actctggtct
     9781 agcccctgat tttaaggtgt cgcgataggc cagcacctct ttcaacgtaa tggagggcaa
     9841 aattttgcgc cgttcatctc gcgagaagta cggcacttgc gagagcatct gcgcgggcat
     9901 aatcgcctgc tcaaacgctt tgcccttttc tgcggaatcc atcatctggt tataccagga
     9961 cttcgcctgc tcaagctgat cttccgtagc ggtatagcta aagtagccct ccagcagagc
    10021 ctggaacagc tgcggcaggc gctgagtgta accattggca ttaaccataa ggccgttgtt
    10081 agcattggtg gaaaaactta tgccaccaac cgacgcctgg ttgcttaact gatcaagcgc
    10141 cagccctgcg agataatcat tgagcgcaaa catcacctga ttgcgggcgc tgtccatggc
    10201 tttcggatta cgcaaaatca ggctgacatc agctttgggc tcgctggcaa aataacggct
    10261 tggcgcatac accacgcgca gattcgactc atcaacaatc aactctggat ggtcgtattt
    10321 cttctctgac ttaatcagcg agaaatcgtc aggaatatag gggttaagct ccggtaagga
    10381 gagcgcaata ttggcggctt ttttctgcca gtcagcgaaa gtttgtgcgc taattttatc
    10441 gacctgatac ggcgcatcga caaagtaagc cgttttgttg tgcggctctt tcgggctgat
    10501 ataccagata cgcgcattct gcggcgtcat catcgccaga cgttctttta ctgctttagc
    10561 atcgtaccga tcggcaatat tgactgcatc cagtgtatgc tcaacaggaa cgcgaatcat
    10621 ggtatctgcc agccattcga cgtaatccat atcacgggtg attgacggat aacggaagtc
    10681 gatatccagc acattcgcca gttcatcgaa gtattgttta tcgatccctt tttcacgtaa
    10741 cagattgagg tagctaaaaa tggccgccac aacctgatcg cgattcgcca gacctttatc
    10801 ggttaaagac gcagagatcg ctaatacgcc gctgttgccg ttaacgatag gatcggaatt
    10861 ggcgctaatg ccctcaacta atccctgctt ttgcagccag tcagaaagtg tacctgggct
    10921 gcgattacca atcagatagg taatcaattc atccgtttta ctacggaact tcgctgaatt
    10981 gttatcgatg cgaaactcaa cgcgcagaac tttacgcggc agcgccggga cgtaatgaat
    11041 gataatgcct ttttgcgcgt cggtgactac cggcacggtg atttccggtt ttttgctctc
    11101 tttgttcggc acgcgaccaa aggtgtccgc cgccattttc gccaactccg gcaacggttt
    11161 attgctgtaa ataaccgcct tcatcagatt ggcggaatag tacttctcgt ggaaatcttt
    11221 cagcgcctgc tgtaccggat tacctggttt gtcgcttaaa gtttcgaggt taccaccaga
    11281 aaactttgaa ccggggtgtg ccgggttaat ggtttctgcg ctgacctgtg ccatgcgcat
    11341 cccgtcacgc gtacgcgcca tggttaattc ggcattcact gcattacgct cacgttcggc
    11401 gtatttcttg tcgagcaagg gttctgcaat agcatcggcc aggcggtcta ccgcaccggg
    11461 taaggcatcg ttctcaactt ccagatagaa agccgtgcga tacggtgccg tgctggcatt
    11521 gtgactgccg ccgtgcattt tgagatattc ggccagactg tcagcctgcg gatacttttt
    11581 cgaccccatc agactcatat gttcaaggta atgtgccagc ccctggtacg cctcaggatc
    11641 ttccagcgac ccaacgggca ccaccagcgc cgagagcgat ttaactgcct gcggatcaga
    11701 aaccagtaag accaccatac cgttatccag acgtatagcc tgatactggc ggttatcttt
    11761 atcactttta cggatggttt cctgaatggg ctgccatccc gtttctgcct gacttaaggg
    11821 tgcccaaagg gcaacaaaca acaataatgc tttgaaccag atgctgcggg gcattcacgg
    11881 acctcataat caactttatt ttctggccag attcaacacg ttaacgcaaa tcatctgcca
    11941 gaatttaatc ttgtgctgca cgagtcagcc tatgtctata taaccatcag tccgtgactg
    12001 gtgcgcatca taaagtaagc ggatagattg cgcaattttt atacaatact catgactgat
    12061 taaagcgaaa cagcggtaac aggaaacgtt gcgactgttc aacgatagcc tccattgtct
    12121 ctggtgttaa ttgccgccag agccgttgat accagatatc atcaccttcg ccacgcacca
    12181 tcatgttgcc ttcgtaagcc tgaaggaatt tcgtacgggc tttttgcaac gtggaatcgt
    12241 catccagcat ggcatcgttt tgcgcgtcat aacaggtttt tagccacgcg ccgccacttt
    12301 caggtaacac cagcaatggc gcggacattc cttcacgata cccctcaatc agttgtgaga
    12361 ggtaatgcaa agcctgttcg gctgcaagcg gcggaaaacg ccactcgccg tctttgcgta
    12421 gaaaaaggcg actttcacca ttaccaccgc tggcacagta gacaaggtgt tccagccaaa
    12481 gttgcattcc ctgcgccaca cttaataagg agggacgcca gcgcaacagg ccatccggct
    12541 gcacctgcgg caaccagccc gttatttgca caccgttgca ggcgagatca atctccatac
    12601 tctgccccgg ctggcgacag gcaatgactc tgtcggcaag ctgctgcatc tcctgacact
    12661 gcgtttccca gaaaatttca ccaaaggcac catacggcaa atcacctgcc gcccggaatc
    12721 ggcggaacaa gcgttcggca tcatcctgct caaccagtac atttaataac tgctggttga
    12781 tttgataacg actaagccct tcaagaataa aaggttcggt gtcaggaatt tcgctgtctt
    12841 cggtacggaa gttcacctgc aaacgcatct ggaaaaacgc ccgcaccgga tgtgcccaga
    12901 atcgttgtag cgtctccagc ggcacagttt ccggcaaggt aaacggcagc ggctgaacaa
    12961 attcagaatg tgctttacca gcctggctgg ccgcaggtag ccactcgcgc gcatagcttt
    13021 gtaagttacc gggctggtag ttttgcgggt caaacggcat ccgggtatgg tggcaagtaa
    13081 gatgcgcttt tacccttgcc tcgctttcat cacagttgag cgcttcatcg cccggtagat
    13141 aatggctttg cccgatgtag tcgatcagtt cctgcaccag taccgacggg aaacgttcac
    13201 tgttatcctg aatggaacgc ccgatatagc tgatatagag tttttgctgc gcggaaatta
    13261 acgcttccag gaacagatag cggtcgtcat cgcgacggct acggtcgcca cgcttcggtt
    13321 tctggctcat cagatcaaag cccaatggcg caagctgacg tggataaacg ccgtcgttca
    13381 ttcccagcag gcaaaccact ttgaacggaa ttgaacgcat tggcatcaga gtacaaatgt
    13441 taaccggtcc ggcgagaaaa cgctggctga tacgttcttg atccaggcgc agtgccagtt
    13501 catcacgcaa tagtgacagc ggcaccgcgt cgccatactg cgcacctaaa ccttcggcga
    13561 taatcgcctg ccattgttgt tcgatcagcg tcatcgccgc ttcggtttcc gcatccggca
    13621 ggaaaaaggc gttgagcata tcgcgacaaa ccggcaacca ctcttccagc ggacgctcct
    13681 gcgccagccc gcgacgccag atatttagct gcattagcag tgaagccaga tgccccacca
    13741 gttctgcaat taagccgctc gattcatcat aaggtagaac cgattgccac tcgccctgcg
    13801 cgctctccat cgcgtagccc agcaacatgc gcgtcaggcc aaaccgccag gtgtgttgac
    13861 cggtagcggg aagttccagc tcgcgaacgt tgtcgtcatc tatcccccaa cgaattccgg
    13921 attcgttgac ccactgacgt aaataacgca gcccttcttc ggtgatgtca aaccgcgctg
    13981 ccagcaccgg cacatccagt aatgccagca cgtcttccga cacaaagcgg ctgtctggca
    14041 gcgataacag gctgataaac gcttcaagta caggatgcga ctgccgcgcc cgacggtcgg
    14101 aaatggcgta aggcaggtaa cgatccgcag gtgcgctacc aaacacagcc tgaataaacg
    14161 gactgtagct gtcgatatca gccaccatca cgatgatgtc gcgcggagta agtgtcgggt
    14221 cttcctccag catcgccagc aggcgatcgt gtaaaacttc aacttcacgc tgcgggctat
    14281 ggcaaacgtg gaaggtgata ctgctatcca gtggatcaag cgggcgtttg ttatcgctac
    14341 gggaaaactc ttcgatgttc acaccagcaa cggcgcggtt ttccagttcc agaatgtcag
    14401 actgaatatt atgcagcagg ttatctggcg tcacatcgac aaaagcgtcc agctcctggc
    14461 tgctctccag gtcagaaagg agataaatgt agtcgcgccc aagcttgccc catgaagcca
    14521 gcagcgggtt gccgacatcc tgttcaccat cgctgttaaa gagctgcccg gcattttcgc
    14581 tgtcgcgaaa tagcggtaat tcgcgatctt caaaactgtg tcggcgctgg cgagtcagta
    14641 gtttcgccag ataagctgga tctttaatgt cgccccagta ataacggcag gggttggtaa
    14701 acaggagatg gatttcaata tgtttaccca gcgcctgtag cgcctggaga taaacaggcg
    14761 gtaacgcgga aataccgcat ataaagacgc gcgaaggtaa ccccggcggg caggtcgtcg
    14821 cggactccag cgtttcgata aagcgctgat agagattggc gcggtgccag cgcggttgcc
    14881 caagttcgtc ggtatattcc accaacgcct tccacaacgg cgcttgccag gcctgtgctt
    14941 ctcccaaccc ttctaccaga tgtcctgttt cccactgtgc cagccagtcc ggacgataga
    15001 ccagatactg gtcaaacagg tccgccgctt ttgaagaaag ctggaacagt tttcgcttgt
    15061 cactatcgtc agtcagataa tgccgcaaca gggtaaagtc ttcgcgctcc aacagttgcg
    15121 gcagcagagt catcagtttc cagctcatgc tctgtttgtt aaaagcgctc tctttgggga
    15181 tctccggtaa cacccggacg aacatatccc agataaagct cgctggcagc ggaaaatcaa
    15241 tgtttgccgc aataccaaac ttttgcgaca gggtcatttg cagccactgt gccataccgg
    15301 tactttgcac cagaatcatc tctggttcga aaggatcgtc cagccgttcg cgttcgacaa
    15361 taaactccat caacgcttcc agcacgtcca gacgattgga atggtagacc cttaacatag
    15421 cggctcctga ctactgacga ttcgggcaat gcaggcgggt catctcgcct tctctgcccc
    15481 cgggtgaaac tagcgtaacg ctgatgctga cacatcccgc ctgcgatgtc tgcattcggt
    15541 tgacctgcca gttagcaggt ggcgaaatcg cgcgcagttg cgtttgctgc cagccatgcc
    15601 gccagagctg ttggtactgg tttctgctgg caagactgtt cattaatgtt cgctggaaac
    15661 ccgataacgc agtgacaatc atcaccatca acaccatcgc caacattacc tccggcagac
    15721 taaagccttg ttgattcctc agggaagctg acataacgcc ctctctttca gcggacaaaa
    15781 atcgctccag ccgcgtggcg aaaaaacaat gtttccatcg atgacttcgc ctgttcgcca
    15841 caacgaaacg ccttcataac cagcaatcaa taaggcttca ttatctgcca gtaaacgcag
    15901 acaaacccgg gcaccggtgg cagcatacag taagcactga actgcgggca gcgtctgcca
    15961 ggagtgcatt tttccccact ccagcgccga ctgaacgatg gcctggcggc gcaatgactg
    16021 actttccatg ctcacgcgag aagcaaaact gcgatcctgc tgactcattc cttgcaatag
    16081 caagctaccc aaaatcagca gcatcaggac cagagccagt gacgaaacac ccttttcgcg
    16141 gttcacaggt tggatcctgt cacgctatag ctggcatcca ccacggtttg cggatcagcc
    16201 ttgctggcag cgtgcatatt aaccgtcaac accggcgaga agccgctgac atcctgacgt
    16261 acgacctgaa aagtgtcgat aatgatggca tccggattag tgactttatc ccagccctta
    16321 ccttcacagg atgtcgcacc gcgtagcgtt tccagcacat gctccttcag acgaaatcca
    16381 atctggtcgg actcttttac cggttcgcga tcccagatac cattactgtt cgcatcccac
    16441 tgcacaatga cacagtcacc ctgtccgaca atttccagcc cttcgcccgt acagctgcca
    16501 tggcaataac ccgccctctg gagatgcttc gcgacggtaa atacccgcag ccagatttca
    16561 tcttccagcg ccagcttacg ggtgttcgtt aaactttcac gctgtaacgc aggcagaaag
    16621 cgtgcagccc ccagcaacaa tacgctactg atcgccatag caatcaacac ttccagcaga
    16681 gaaaaacctt gctcttttac aggcatcctt ctgtttctcc ttgctgacaa agccggagtc
    16741 ttccccacgg cgaaaccact agccaccact cgcccgttga gtttttgaag cgaatatgcc
    16801 cggcccatgc ggtattgcgc aggccaaaga aagcaagcga aggtgtcagg tcgctcattt
    16861 cgacttcggg ccagcgtggc ataaagacca atggtgaact gccatgacag gtattcgccc
    16921 cagcaacgga actcacaagg caccataacg tcccctccct gataagactg atactgtggt
    16981 cgcggttatg ccagttggca tcttcacgta aatagagcaa atagtcccgc gcctggctgg
    17041 cggtttgcca aagccgttgc gactgctgcc agtattgcca gccatagagc ccacttgcgc
    17101 ttagcatgac cagaatcagc atcgcgacca gcgtttcaat cagcgtataa ccacgttgtg
    17161 ttttcatgcc ggcagtatgg agcgaggaga aaaaaagacg agggccagtt tctatttctt
    17221 cggcgcatct tccggactat ttacgccgtt gcaggacgtt gcaaaatttc gggaaggtgt
    17281 ctcgaagaat ttaacggagg gcaaaaaaaa ccgacgcaca ctggcgtcgg ctctggcagg
    17341 atgtttcgta attagatagc caccggcgct ttaatgcccg gatgtggatc gtatccttca
    17401 atctcaaagt cttcgaaacg gtagtcgaag atggattcgg gtttacgttt gataatcaac
    17461 ttcggcagcg gacgcggttc gcggcttaat tgcagatgag tttgatccat atggttgctg
    17521 tagagatgcg tgtcgccacc ggtccagaca aaatcaccca cttccagatc gcactgctgc
    17581 gccatcatat gcaccagtaa cgcgtagctg gcaatgttga acggcaggcc gaggaagacg
    17641 tcacaggagc gctgataaag ctggcaagag agtttgccgt ctgccacata gaactggaag
    17701 aatgcatggc acggtgccag cgccatttta tccagttcgc ctacgttcca cgctgaaaca
    17761 ataatgcggc gggaatccgg gtcgtttttc agctggttca gtaccgtagt gatctggtca
    17821 atatgacgac catccggcgt tggccaggcg cgccactgtt taccatacac tggcccgagg
    17881 tcgccgtttt catcggccca ttcgtcccag atggtgacat tgttttcgtg tagataagca
    17941 atgttagtgt cgccctgcag gaaccacagc agttcgtgga tgatggaacg caggtggcaa
    18001 cgtttagttg tcaccagcgg gaatccatcc tgcaggttaa aacgcatctg atgaccaaaa
    18061 atggaaagcg ttccggttcc ggtacggtcg tttttctgtg tgccttcgtc gagcactttt
    18121 tgcatcagtt ctaaatactg tttcatggtt cctcaggaaa cgtgttgctg tgggctgcga
    18181 cgatatgccc agaccatcat gatcacaccc gcgacaatca tcgggatgga aagaatttgc
    18241 cccatgctga tgtactgcac ccaggcacca gtaaactgcg cgtcgggctg gcggaaaaac
    18301 tcaacaatga tgcgaaacgc gccgtaacca atcaggaaca agcctgagac agctcccatt
    18361 gggcgcggtt tacgaatata caggttgagg ataataaaca gcaccacacc ttccagcagc
    18421 agctcgtaaa gctgtgatgg gtggcgcggc agcacaccgt aagtgtcgaa aatggattgc
    18481 cactgcgggt tggtttgcag cagcaaaata tcttctgtac gggagccagg gaacagcatg
    18541 gcaaacggga agttcgggtc aacgcggccc cacaattcac cgttaataaa gttgcccaga
    18601 cgcccggcac caagaccaaa cggaatgagt ggggcaataa aatcagagac ctggaagaag
    18661 gaacgtttag tacggcgggc gaagataatc atcaccacga taacgccaat caggccgccg
    18721 tggaaggaca tgccgccgtc ccagacacgg aaaagataca gcggatcggc cataaactgc
    18781 gggaaattgt agaacagaac ataaccaata cgtccaccga ggaagacgcc gaggaagccc
    18841 gcatagagta agttttcaac ttcatttttg gtccagccac tgcccggacg attcgcccgt
    18901 cgtgttgcca gccacattgc aaaaatgaaa cccaccagat acatcagtcc gtaccagtga
    18961 agcgccacgg gtcctattga gaaaatgacc ggatcaaact ccggaaaatg cagatagcta
    19021 ctggtcatct gtcaccacaa gttcttgtta tttcgctgaa agagaacagc gattgaaatg
    19081 cgcgccgcag gtttcaggcg ctccaaaggt gcgaataata gcacaagggg acctggctgg
    19141 ttgccggata ccgttaaaag atatgtatat gatccgcgct ataacccccc gcgaatcagc
    19201 ccgcccatgc cgcgacgctc cataaaggca gcaacctgat ggcgaacttc ggtcgccagt
    19261 tgcgcttcca gactacgctg cgcaagattt tctgcttcag caaaatcaat gcgccgcagc
    19321 aggtatttta cgcgcgctac agaacgtccg ttcatagaca gatggcgata cccaagccca
    19381 atgaggattg ccacgcacat gggatcgccc gccatttcac cgcacaaacg gagatcgatt
    19441 ccatgtattt ccgcttcccg ggcgatcatc gccagagctc gtaacattgc aggatgaaga
    19501 ctgtcataaa tgttcgccac ccgggtattg ttgcgatcaa cggccaggat gtattgagtc
    19561 agatcgttgg tgccaacaga gatgaaatcg acccgctttg ccagatgcgg cagcataaat
    19621 accattgacg gcacttccag catgatgccg atacgtggtt tgggaatttc gtaaccgatc
    19681 atctcctcga cttcacgtcc ggcacgttca atcaggcggc gtgcttcgtc aacttcatcg
    19741 aggcttgtga ccatcggcaa cagaatattc aggttgcccg tagcggcatt agcacgcagc
    19801 atcgcccgca cctggatcaa gaagatctcc ggctgatcga gcgtaatgcg aatcccacgc
    19861 caacccaggc atggattctc ttcgctgatc ggcatgtaag gcagctgctt atctgctccg
    19921 acatccagcg tacgcaaggt gacgggttta tcattaaaca tttgcagcat cccctgatac
    19981 tgcgccacct gttcttcttc cgacggaaaa ccactttgca gcatgaatgg gatttcagtg
    20041 cgataaagtc ctatgccatc aatacggctg cccagttttt cttcatgttc cgggcttaaa
    20101 ccggcattga gcatgacttt aatgcgttcg ccgcttttta actgggcggg taaattgacg
    20161 tcatcttccg ccagacggct aagctcgatc tcttcactaa ttagccgctg atattcttgc
    20221 agcagtaccg gttccggatc gaccagcaat tcaccgcgat aaccatcgac gatcagcgtc
    20281 cgacgatgca gtaccgaagg ctgaatatcc gcgcccatca cggtaggtat ccccagcgca
    20341 cgtaccatga tcgcagcatg ggagttggca gcgccatcgc gcacgacaac accgactaag
    20401 cgatcctggg gcagctcagc aagcgttgtc gctgacagtt catctgccac cagaatgaaa
    20461 cgttccggcc aggcgttcgg cccttgatta gcgtcatcaa gatgaaacag caatcgctga
    20521 cccagcgcac gtaaatcgcc agcccgctct ttgagatagt tatcgcttag cgcggcaaac
    20581 tgttcggcaa atttttcaat gaccgttttt accgcccact ctgccaccga gcctttatca
    20641 acctcggcaa acaattcgcg acgcagccgg gtatccgaaa gcaggtgtga gtaaagatcg
    20701 aaaatagccg ccgtttcttt ttgtgcaccg gcagcaaagc gtttgctata gcggcgaaac
    20761 tcgttagccg cctcttccaa cgccccggtc agtcgttcgc gttccagagc cggatccagc
    20821 gttgatgcct gatacacctg ttccattaaa ggtaacgtgg catcctgcca gccttcggca
    20881 atcgccacac caggtgctgc cggtaatgca cggattcgcg tctggcgata ttgcccaaac
    20941 aaggcagtca actgcgactg agaaagaata gctgccatct gggtggcaag cgtcaccagg
    21001 aaggattctt cactttcgtc atactggcgc aactctcgtt gctgtaccac cagtacacca
    21061 agcaactggc gacgttgaat aattggtacg cctaaaaacg cgcggaaacg ttcttctttt
    21121 acggagggga tgtatttgaa gctggggtgc ttttgcgcat ctgcaaggtt tatcggttcc
    21181 gccagcctgc caaccaggcc gacgatccct tcatcaaacg cgagcgttac agtgcgaccg
    21241 cgtggttttt tcagtccccg ggtcgccatc aggtagtaac aacgtcgatc atgatcggcc
    21301 aggtagaccg aacagacctc ggtatccatc gcaagacaga tgtcggtaac cagaatattt
    21361 aacgcctcat tcaggcgtgg tgcgctggct accttttcga ctatttcgcg caggcgagtg
    21421 agcataattg gcgtgactta acctctttta cgtcgataag cagatgcgtt ttgtggtttt
    21481 ggcgtatttt cctgcagtga catcaccaca ctcgcgaact ctttcattac cctacggtag
    21541 acatcacgtt taaatgacac cacctgtctg actggatacc agtaacttac ccatcgccag
    21601 ccgtcaaact ctggtgtact gctggtttgc atattgattt ctgcatcgcc gctcaccagc
    21661 tgcaagagaa accatttttg tttttggccg atacaaaccg gcttcgtgtc ccaacgcacc
    21721 aaacgtttcg gtaatttgta gcgcaaccag ttacgcgttg aagcaaggat tcgaacgtct
    21781 ttgcggctta atcctacttc ttcaaacaat tcacggtaca tcgcctgctc tgcggattct
    21841 ccggggttga ttccgccttg cggaaattgc caggagtgct gaccaaatcg ccgggcccac
    21901 attacctgcc cctggcgatt acaaatcacg atacctacgt ttgggcggta gccatcgtca
    21961 tcaatcaccg gactacctca aaataaagct ttatatacga atgattgttt catactccag
    22021 gaagacggta aaccactctc tgcagggcat tacacactaa taacaattga ataactcaca
    22081 gttatgtgca gagttataaa cagaggaagg ggtggatagc cgtttttatt caccttttct
    22141 gtggatagag ttgtgaagaa ctacggaatt actgcgggaa aacccggagc attctgaata
    22201 agacagatat agagatttta aaatattcat tatcagtcaa tgcattacat gtgatttgag
    22261 taataaaaaa gagtaattcg tgacccaggt cacacctctc atttacgggt tggcgaaaga
    22321 tcaaccaatg ccgtatttat ccacagaatg tgccactaag ttaagcactg aaccactaaa
    22381 aactggagtt tcgtcgcacg tcaaggctgt aaatggaaac agtagtggag gtttttcaca
    22441 gttatcccag ctttctgtgg ataacatggt gtaagatcct gtttattttc agtgaccaga
    22501 tttggaaaac ccgttgcagt gttgcgcaac tcgtttaccg gcaacctaaa aagcaatata
    22561 aatcagagaa ttgaacaacg catgtggaaa aagttacact gcgaatattc agcacataat
    22621 tgctgtttgt tttttaatca aggtatcatg acatgtccca acctcgccca ctgctctctc
    22681 ctcccgaaac tgaagaacaa ttgttagcgc aagcacaaca actttctggt tatacattgg
    22741 gagaactggc ggcacttgcc gggctggtta cgccggagaa tttaaaacgc gataagggct
    22801 ggattggcgt gttactggag atctggctag gtgccagcgc agggagtaaa cctgagcaag
    22861 attttgctgc tctgggcgtg gaacttaaaa ctatccctgt ggatagtctt ggtcgtccgc
    22921 tggaaacaac attcgtttgt gttgccccgt taacgggcaa tagcggggtg acctgggaaa
    22981 ccagccacgt gcgccacaag ctcaaacgcg tgctgtggat accggttgaa ggcgagcgca
    23041 gcatcccgct ggcaaagcgt cgcgtaggat caccgttgct gtggagcccg aatgaagagg
    23101 aagaccggca gttacgcgaa gactgggaag aattaatgga tatgattgtt ctcggtcagg
    23161 ttgagcggat caccgctcgg cacggggaat atttacagat acgaccgaaa gcagcgaatg
    23221 cgaaagcgct gaccgaagct attggtgctc ggggcgaacg gattctgacg ctgccgcgcg
    23281 gattttattt gaagaagaat ttcaccagtg cgctactggc ccgtcatttt ctgatccagt
    23341 agccatcgct ttgacctgcc gctttccggg catataatta ccgcttcatt tttttggcag
    23401 ggctttttag atgttatttg catggataac cgatcctaac gcctggcttg cgctcggtac
    23461 actgacgctg ctggagatcg ttcttgggat cgacaatatt attttccttt ctctggtggt
    23521 ggcaaagctt cccacagcac aacgtgctca tgcgcgccgt ctggggttgg cgggagccat
    23581 ggttatgcgt ctggcgctgc tggcatccat cgcctgggtt acgcgcctga cgaatccgct
    23641 ttttacaata ttcagtcagg aaatttccgc ccgtgatttg atcctgcttc tgggtggctt
    23701 gttccttatc tggaaagcca gcaaggaaat ccacgaatcc atcgaaggtg aagaagaagg
    23761 gctgaaaaca cgcgtttcat cattcctcgg cgctatcgtg cagattatgc tgctggatat
    23821 tatcttcagc ctcgactcgg tgattaccgc tgtgggtctg tcagatcacc tgtttattat
    23881 gatggctgcc gtggtgattg ccgtaggcgt gatgatgttc gccgcgcgct cgattggtga
    23941 ttttgtcgag cgccatcctt cggtaaaaat gctggcgctc tctttcctga ttctggtggg
    24001 ctttaccctg attctggaaa gtttcgacat ccacgtaccg aaaggttaca tctacttcgc
    24061 gatgttcttc tctattgcgg ttgaaagcct aaacctgatt cgcaacaaaa agaatccgct
    24121 ctgatactcc gtacgctctc ctgcgggagg gcgtactcac tcctgttacc ctcacagttt
    24181 aagattttcc tgctttcaag atatataacg tcagtttata aacagactat tatcataggt
    24241 gggccaaatg aggataaccg gatgaaaaaa tgggcagtaa taatttctgc agtcggactg
    24301 gcgtttgctg tttccgggtg ttccagtgat tacgtcatgg cgaccaaaga tggccgtatg
    24361 attttgaccg atggaaaacc tgaaattgat gatgataccg ggctggtgag ttatcacgat
    24421 cagcaaggta acgcgatgca aattaaccgt gatgatgttt cgcaaattat tgaacgttaa
    24481 caaataaggt cagcatccgg ctggccttaa gatttttctc tttccctttt cccttccctc
    24541 tgccattttt atattcctta tgtcgtgatt ataaaaagga aacggctatg caatatcacc
    24601 gtatacccca cagttcgctg gaagtcagca cgctggggct tggcacgatg acgtttggtg
    24661 aacagaacag cgaagccgac gcccacgcac aactcgacta tgccgtcgct cagggcatta
    24721 accttatcga cgttgccgaa atgtacccag tacctccgcg ccccgaaact caagggctaa
    24781 ccgaaaccta cgtcggcaac tggctggcga aacatggcag ccgcgaaaag ttaattatcg
    24841 cctccaaagt gagcggaccg tcgcgcaata atgacaaagg catccgcccg gatcaggcgc
    24901 tggatcggaa gaatatccgc gaagcgctgc atgacagcct caagcgtctg cagactgatt
    24961 acctcgatct ctatcaggtg cactggccgc agcgcccaac caactgcttc ggcaaactcg
    25021 gttatagctg gacagattct gcgcctgcgg tttcgctgct ggatacgctg gacgcactgg
    25081 cagagtacca acgcgcggga aaaattcgtt atatcggcgt gtcgaacgaa actgcatttg
    25141 gcgtaatgcg ctacctgcat ctggcggaca aacacgatct gccgcgtatt gtcaccattc
    25201 agaaccccta cagcctgtta aaccgcagtt ttgaagtagg tctggcagaa gtcagccagt
    25261 atgaaggggt cgaactgctg gcctattcgt gcctgggttt cggcacactg accgggaaat
    25321 atctcaatgg tgcaaaaccc gctggcgcac gtaatacgct ctttagtcgg ttcacccgct
    25381 atagcggtga gcaaacgcaa aaagccgtcg cggcgtatgt tgatatcgcc agacgtcatg
    25441 gcctggaccc tgctcagatg gcgctcgcat ttgtacgccg tcaaccgttt gttgccagca
    25501 ctctgctggg cgcaaccacg atggagcagc tgaaaactaa cgtcgaaagt ttgcatctgg
    25561 agttgagcga agacatatta gctgaaattg aagcggtgca tcaggtttat acgtatcctg
    25621 caccataaga ttaacgccgg ttaaaaccgg cgttaaatat taatgacggc gctgccagat
    25681 ccacagcgcc gttattgcca gcgcaaacag cgcaccgaag ccaatgccaa tgggcacgac
    25741 cgggatgcct accattaccg ccagcgagta aatgcccagc atcaacaaca tggcgctgtt
    25801 ttcgccaagg ttttgtactg caatcgcatt ccccgccccg acgctttttt taccccgctc
    25861 ctgtagcaac gcattgagcg gaacgacaaa aaagcccccc agcacgccaa tcagcatcaa
    25921 caaagcatag gctggcagca gctcgtgttg cagggagaaa atcagtacca ccacgccaat
    25981 caaaatcccg gctggcatac agcgtgacac ggtttccagc gtaaccagct tcgctgccgc
    26041 ccctgcgcca accacgatac caatcgctac catcgcgttg agataggtcg gcgtagcgtt
    26101 atcggtaatg cccagcgcca ccggtaccca cagcaccaac aggaaacgca gcgtgacacc
    26161 tgctccccag aataaactgg tgcccaccag cgaaaaacgc gtttcaccat tgcgccatag
    26221 cgaggtgcag gcattcagga aactgcgggt catgttgatg agattccagg actgccccgg
    26281 acgcgccgcc gccagtttgg gaatgtagat attggcaacg accgcaccac cgtaggccag
    26341 tgcgcatgcg gccagggcga ccaggacatg ccagtcagcc agtacaccac cggctacgga
    26401 accgagcaaa atcgccgcta tggtagaagc ttccattaaa ccgttagctt tcactaactt
    26461 actacccgtg gttaattcgc cgagaatacc gtatttcgcc ggtgaatagg ctgcagcacc
    26521 aacacccacc agcgtatagc cgagaaacgg attgatacca aagcagatac tggctgcgcc
    26581 cagtagcttc aggccgttgg caaacatcat cacccggcct ttggcgaagc tatccgccac
    26641 ctgcccgaca aacggcgcaa aaagaatgta agcacctaca aacaccattt gcaggatggg
    26701 ctggctccac tccggataga actgcgcttt cagtaacgcc agagtggcaa acagtagcgc
    26761 attatcgcca aacgcagaga gaaactgcgc cacgataacc gctttcatcc ccttcgacca
    26821 caacgaagtg ttagtgtgca ctgactcact catcgtgttg ttccgcttcg tctacccagc
    26881 ttttcaacgt gacaaagtca ggtttgccgc tgccaagtaa tggcatctgt ttcagatagc
    26941 gaatatcgcg cggtacagca agctccggca cgccgtgctc gcgggcatac tgttgcaact
    27001 tatcgcgcat cagttcgtta tctgtggtga aaagcaccag tgcctcgcct ttgctggcat
    27061 cgctcttaat cgcagtggca tggactttat ctggcgaaac accaagtgcc agttgttcca
    27121 ccatttccag cgacaccatt tcgcctgcaa ttttggcaaa gcgttttgcg cggccctgaa
    27181 tctgcacaaa gccctgctcg tcaaaacgca caatatcgcc agtgtcatac cagtcgcgct
    27241 ccatttcgcc gcgaatattc tcggcggtgg gcacttccag tacacctggc ttctccaccc
    27301 gcagatagcc gttcattatg ttcggccctt ttagttgcag gcgaccgcct tcttctatgc
    27361 ccggaaccgc cagcagacga gcatccatcc ccggcaaaat acgcccgacg gtgccaggtt
    27421 tcgccgccat cggtacgttg atagaaacga caggcgcgca ttcggtcaca ccgtagcctt
    27481 caaggatgcg caggccaaat ttatcctgcc aaagctgttt agtactttct tgtagttttt
    27541 ctgcgcctgc caccacatag cgtagacgat agaagtcata cgggttggcg aaacgcgcgt
    27601 agtgaccgag gaaagtggag gtaccgaaca acacggtgca actgcggtca tacaccaact
    27661 ccggcacaat gcggtaatgc agcgggcttg gataaaggaa cacttctgca cctgtgagca
    27721 gtggcgtaaa caggcctacc gtcagcccaa aggagtgaaa cagcggtaac gccgacataa
    27781 agcgatcgtt ggtggtgaag tcggcaatcg ttttaatctg ctcgacgttc gccagaatgc
    27841 ttttatggct atggacgacg cctttcggat ggccttcaga accggaggta aagaggatca
    27901 gcgcctcttc ttccggctgc tgttttacct gtgccagacg cggcatcagc aaatgagcga
    27961 aaatccacac tttgtcggca gtgttgacat ctgcttttaa atcttccaga tagacccagc
    28021 gcacctgagt aagttgctcc ggcagatgcc agagtttgcc tttatcgaga aactgacggg
    28081 aagtgaagat ggttttgatt tcagccgccg taatagcact ggtcaaccct tttaccccgg
    28141 cggtgtagtt catcattgcg ggaatgcggc gacgggcgat ggccccaaaa atcactgccg
    28201 cactgatgcc tgcattgggc agcattaagc cgatgcgttc gccttcaaca ctgtattttt
    28261 caaggatgcg tccaacaaac agcgttttcg taagcaattt gcgataggag tccggggtaa
    28321 agttgacgtc ttcgacacat ttcttcccgg ctccgaagcg gtacattgcg ctcagtaaag
    28381 attcgtacag cgtttcacgc gggcgcaccg ccatacgcgc ctccatcatt atttgatgca
    28441 gcatttcgcc agcgattttg cgacggtcac gggcacgcgg cgcatccggc atctccacct
    28501 gcgttggtgg caaaatatgc agagtaattt gcgggaacaa gcgacgttta accagacctt
    28561 tcaggcggct gaagtgcgta agttccgccc cttcaatacg cacaggaata accgttgcac
    28621 cagacttcgc cgcgacaaaa cccgcgccat cgtagatttt catcagcgag ccagtggtgg
    28681 tgatgcgtcc ttcagggaaa atcaccactg gtcggccctg ttccaccaga cgtaccagat
    28741 gtttaatcgc cataggttgc gtcgggtcga gaggaacaaa gtcgataaat gatttcagcc
    28801 aacgcatata ccactgttgg cttattgagg tgtaaacggc aaataccgga cgtacgggta
    28861 aaaacaatgc cagcagtatg ccatcaataa aagagacgtg attaggcgtg attagaacgc
    28921 gctcgccctt cagtgcctgg gtgtcacccg taacgcgaac gcgatacaaa acacggcaca
    28981 aatttcgaaa aaagctaaaa agcattccaa ctccctttgc tctgattcag taaaagcgaa
    29041 tggagggaga ttacacgaga taaagaacgc gagcgacagt aaattaggtg cgaaaaaaaa
    29101 cctgcgcatc cgcgcaggtt ggtgcaagag actgggtacg aagagcgtac cgaataatct
    29161 caccaatcaa tacctctggg atcttgattg tggtctgcac aacgactctt cgccagcgag
    29221 aaaacgcaaa ggaatgaagg gaaatgcaac gaggtgtgta aattgtcggt tactgttaca
    29281 gattgatgac cggcaaaaaa aaacctgcgc atctgcgcag gctggtgtaa ttcatgtgct
    29341 caacccgaag ttgacttcac ctatcaatac ctctgggatc accactttag caacctgaag
    29401 ccaaacgcca ccagcggtca atcgcaacag cgtttcgcaa agtgtaacca aaggtttgca
    29461 atctcttttt ttgtcttact tatttgcgtt tggctcacat tcccacaatg aaaacacgcc
    29521 gccccttgaa ccaacgggcg ttttccgtaa cactgaaaga atgtaagcgt ttacccacta
    29581 aggtattttc atggcgacca taaaggatgt agcccgactg gcaggcgttt cagtcgccac
    29641 cgtttcccgc gtcattaata attcacccaa agccagcgaa gcttcccggc ttgctgtgca
    29701 tagtgcaatg gagtctctta gctatcaccc gaacgccaac gcccgtgcgc tggcgcagca
    29761 gaccactgaa acgatcggtc tggtcgttgg tgatgtttcc gatccgtttt tcggtgcaat
    29821 ggtgaaagcg gtcgaacagg tggcttatca cactggtaat tttttattga ttggcaacgg
    29881 ttaccacaac gaacaaaaag agcgtcaggc cattgagcaa ctgatccgcc atcgctgtgc
    29941 tgcgctggtt gtccatgcca aaatgatccc ggatgccgat ttagcctcat taatgaaaca
    30001 aatgcccggt atggtgctga tcaaccgtat cctgcctggc tttgaaaacc gttgtattgc
    30061 tctggacgat cgttacggtg cctggctggc aacgcgtcat ttaattcagc aaggtcatac
    30121 ccgcattggt tatctgtgct ctaaccactc tatttctgac gccgaagatc gtctgcaagg
    30181 gtattacgat gcccttgctg aaagtggtat tccggccaat gaccggctgg tgacatttgg
    30241 cgaaccagac gaaagcggcg gcgaacaggc aatgaccgag cttttgggac gaggcagaaa
    30301 tttcactgcg gtagcctgtt ataacgattc aatggcggcg ggcgcgatgg gcgtgctcaa
    30361 tgataatggt attgatgtac cgggtgagat ttcgttaatt ggctttgatg atgtgctggt
    30421 gtcacgctat gtgcgtccgc gcctgaccac cgtgcgttac ccaatcgtga cgatggcgac
    30481 gcaggctgcc gaactggctt tggcgctggc ggataatcgc cttctcccgg aaatcactaa
    30541 tgtctttagt ccgacgctgg tacgtcgcca ttcagtgtca actccgtcgc tggaggcaag
    30601 tcatcatgca accagcgact aaccgcagtt aaagcaattc cagcgccagt aattcttcaa
    30661 tggtctggcg acggcgaatc aaccgcgcct gaccattatc aaacagaact tctggtaaca
    30721 gcggacggct gttgtagttg gatgacattg atgcgccata tgcccctgta tcatgcagta
    30781 ccagataatc acccgccttc acttccggca aggcgcgggt ttcgacattt cccccttcct
    30841 gctgggtaaa gacatcgccc gattcacata acggcccggc gacgacggtt tccaccgttg
    30901 gcgcgtgttc cagagaacga ccctcagctg ccagggcact gatatggtgg taactaccgt
    30961 acattgccgg gcgcatcaga tcgttgaacc cggcatcaac cagcacaaag tggcggctac
    31021 ccatttgttt gacgctccgc acttgagtaa ttaatacgcc agcctgcgct accagaaagc
    31081 gacccggttc aatttccagt ttcacaggat ggcccaaatg gcgggcgatt tgctcacgcg
    31141 cggcattcca cagaccataa taatgttcgg tatcaaccgc ctcttcaccc tgttgataag
    31201 gaatagaaag cccaccgccc gcagaaatag cctgtaaatc ctgaccgaac tcgaggacct
    31261 gacgcaccat agcaccacat acctgttcca gatgggcata atcgacgcca gaaccaatgt
    31321 gcatgtgaat gccgaccagc tgtagatgat gacgttgtat cacgtccagt gcggcgggca
    31381 gatcggtgta ccagataccg tgcttgctgt tttcgccacc ggtattggtt ttttggctat
    31441 gcccgtgacc aaaccccgga ttaacacgca gccatacccg atgccctgga gaaacctgac
    31501 ccagttggtc gagcatatca acagaacccg cattcaccgg aatttgcaat tcactgacgc
    31561 gttcaagcgt cgcctgatcg ataacatctg ccgtaaaaac aatatcatcg gggtgcgttt
    31621 gcggattgta acccgccgcc agtgcacgct ctatttcgcc taacgagacg gaatccactt
    31681 ttacgccctg ctcacgcatt aagcgcaaaa tatgaatatt ggaacaggct ttctgtgcaa
    31741 agcgcaccac atcaaactgt ttcagcgctg caatctgccg acgaataatt tgcgcatcgt
    31801 agacccacac cgggcagcca aattctgcgg gcaaacgcag cagattttcg gcggtgagat
    31861 cggtatcagt gctgaacagt gaatgtggca taacaaactc cagataagtg cttttttatg
    31921 attacgccac atgataaaaa gaataaaaaa tatcgattta tgtcgagtct atgcaaaaat
    31981 gatatggatc accggaatgc gagagagcgc taatggccgc cgttaactta cgtcatattg
    32041 aaatttttca tgcggtaatg accgccggaa gcctgactga ggcggcacac ctgctacaca
    32101 cctcacagcc aaccgtcagc cgcgaactgg cgcgctttga gaaggtgatc gggctgaaat
    32161 tgtttgagcg catacgtgga cgattacatc ctaccgtgca aggactgcgt ctgtttgaag
    32221 aagtgcaacg atcctggtac ggactggatc gcattgtcag tgccgcagaa agtctgcgcg
    32281 agtttcgcca gggagaactg tctattgcct gcctgccggt cttttcgcaa tcttttttac
    32341 cgcagctcct gcaacccttt ctggcacgtt atcccgatgt cagcttaaac atcgtgcccc
    32401 aggaatcacc gctacttgaa gagtggctct cggcccagcg tcatgattta ggactcactg
    32461 aaacgctcca tacgcctgcg ggaacagaac gtaccgaatt actctcttta gatgaagtgt
    32521 gtgtgttacc tcctggccat ccgctggcgg taaaaaaggt attaacgccg gatgattttc
    32581 acggtgaaaa ctacatcagc ctttcccgta ttgacagcta tcgccagttg ctggatcaat
    32641 tgtttactga gaatcaggtt aaacgacgca tgatcgtaga aacccacagc gccgcgtcag
    32701 tctgcgcaat ggtacgggcg ggggtaggcg tttcggtagt taacccactc accgcactgg
    32761 attatgcggc aagcggttta gtggtgcggc ggttcagcat tgcggttccg ttcactgtca
    32821 gcctgatccg ccccctgcac cgcccgtcat cagcgctggt gcaggagttt agtgagcatt
    32881 tacaagcggg attaccgaaa ctggtcactt ctcttgacgc tattttgtcg tcagctacga
    32941 cagcataaaa gcgacagcat cctcggcatg gatcgccgcg gtatcaaaca caggcagaac
    33001 actgcgctct tctggcacca gtaaaccaat ttctgtgcag ccaaaaatga cgccctgtgc
    33061 gccctgttct gcaaggcgag caatcacttg cgcataataa gcgcgtgacg cttcggtaaa
    33121 ttgccccaga cacagttctt caaaaataat ctgattaatt tttgcccgct catccgcttc
    33181 aggaataaga cagttgatgg aaaattgttc cgtcagccgc ccgcgataaa aatcctgttc
    33241 catggtgtaa cgtgtaccca gcagcgccac acgagtcatt cctgccccgg taattgcacg
    33301 tccagtggca tccgctatgt gtaagaaagg cagagagcaa cgagactcaa tggcatccgc
    33361 cactttgtgc atcgtattgg tgcataacac aataccttct gcgcccgccc gctgtaagcc
    33421 aagcgccgcc tcagccagaa tatccccggt tttgtcccac tcaccgcgac gctggcactc
    33481 ttctatttca tgaaaatcga cgctatgtag cagcacttgc gcagagtgaa gcccaccaag
    33541 ccgctgttta atgccttcat ttatcagacg atagtaagga atagtggatt cccagctcat
    33601 tcctcccagc aaaccaattg ttttcattcc tctctcctga tgtgtgttac cgcaacaagg
    33661 ctgaataagg aacgagacaa acgcctcaac ggccaggtgc ccaatctcta ttaacgaaaa
    33721 aagggccgga tgtacagcac atccggcccg tgaaatcaga cgccgatatt tctcaacttt
    33781 tcgcctgcca tcagtttgcg ctcgatatgt tccagcgtga catttttggt ttccggaatg
    33841 agccagaaag tgatgcccac aaacgcaatg ttcagcgcag tgtagagcca gaacgtaccg
    33901 gcagcgccaa tgctatcaag cagtgtcagg aaggtcgcgc cgataatcat attcgacacc
    33961 cagtttgtcg tcgttgagca ggtgatgcca aaatcgcggc atttcagcgg ctgaatttca
    34021 gagcacaaga tccacaccac tggcgcggcg ctcatcgcat aaccggcaat acacatcatc
    34081 gtcatgccaa cagagagcca ggacaagcca ctggaagccg taccgttatc aaactgcatc
    34141 aggcagtaac ccagcaccag agtgcctaac gccatcacgc tgaaaccaat tttcagagcc
    34201 ggcttacgcc ctgctttatc taccgtaaag accgcaatga aggtagcgaa cataaaggtc
    34261 agccccacga ccagagtcgc aatcatctgt tgttctgtgg tcgtaaagcc cgccattttg
    34321 aagatacgtg gcgcgtagta catgatgatg ttcataccgg taaactgctg catcgcctgc
    34381 aacaacatac cgagaaatac agcacgacgg acgttacggt tgatcttaaa cagtgcccaa
    34441 ccgccctgtt ttaacttcag gctttcacga atttcgttga gttcttctcg cgctttttcc
    34501 gacgtatcgc gcagcatacg caatacttct tccgcctcaa tatgacgccc cttttccgcc
    34561 agccagcgcg ggctatttgg caggaagacc accagaataa tcagcagaac tgctggtaaa
    34621 gcaagaaccc ccaacattgc gcgccagtta ccgctataac tgaacgctgt atcggataaa
    34681 aacgccagca cgatgccgag agtgaccatc aactggtaca tgctgatcat cttaccgcga
    34741 acgttttcac ttgccatttc agaaagatat agaggagcgg tgtaagacgc gatcccgaca
    34801 gcaatgccca acaccacacg agcggcgatt aacatctcta cgctggtcgc aaaagcggac
    34861 cctatcgaac cgagtacaaa caggatggcc ccagccatca ggctgtattt acgccccagg
    34921 cggaacgaca gccaaccatt aaacagcgca ccaattgctg cgccgagcat catgctactg
    34981 accacccatt cctgcaaacg actggtcagc acaaagtgat cggtaatgaa cggcaacgct
    35041 ccggcgatta cgccgatatc aagaccaaat aacaatcctg cgactgcagc agctaccgaa
    35101 acaaacatat tcatacgccg cgtatcccgc agaggacgtg gcgttaaagc agattccgta
    35161 ttgatagtga ccattttttc ctgccagcag agagtaagac atagtgaaaa aatacgtgaa
    35221 caattcacac aggtgtcagg tcggaaatag cataaatatg gattaaattg ctacgacatg
    35281 tcgttatgtg atggatattc caattttcaa attaagttga attattgaga ttattattaa
    35341 ctacctaatt ttaaagcaga taaaattcat aaagttcatc aattgataa
//
LOCUS       DADSYM010000032        34852 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2415
            SAMN08039579-rid11723173.denovo.032, whole genome shotgun sequence.
ACCESSION   DADSYM010000032 DADSYM010000000
VERSION     DADSYM010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039579
            Sequence Read Archive: SRR6362533
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 34852)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34852)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980162.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 03:39:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,484
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 4,347
            CDSs (with protein)               :: 4,347
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 43 of 85
            Pseudo Genes (incomplete)         :: 37 of 85
            Pseudo Genes (internal stop)      :: 19 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34852
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039579-rid11723173.denovo.032"
                     /strain="LGE2415"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8302 N 95.9628 W"
                     /collection_date="2015-08-24"
     gene            48..1703
                     /locus_tag="J1Y83_001310"
     CDS             48..1703
                     /locus_tag="J1Y83_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylsulfatase"
                     /protein_id="HBA7629475.1"
                     /translation="MEFSFSPKLLVVAVAAALPLIASAADTPSTATARKGFAGYDHPN
                     QYLVKPATTIADNMMPVMQHPAQDKETQQKLAELEKKTGKKPNVVVFLLDDVGWMDVG
                     FNGGGVAVGNPTPDIDAVASQGLILTSAYSQPSSSPTRATILTGQYSIHHGILMPPMY
                     GQPGGLQGLTTLPQLLHDQGYVTQAIGKWHMGENKESQPQNVGFDDFRGFNSVSDMYT
                     EWRDVHVNPEVALSPDRSEYIQKLPFSKDDVHAVRGGEQEAIADITPKYMEDLDQRWM
                     EYGVKFLDKMAKSDKPFFLYYGTRGCHFDNYPNAKYAGSSPARTSYGDCMVEMNDIFA
                     NLYKALEKNGQLDNTLIVFTSDNGPEAEVPPHGRTPFRGAKGSTWEGGVRVPTFVYWK
                     GMIQPRKSDGIVDLADLFPTALDLAGHPGAKVANLVPKTTFIDGVDQTSFFLGTNGQS
                     NRKAEHYFLNGKLSAVRMDEFKYHVLIQQPYAYTQSGYQGGFTGTVMQTAGSSVFNLY
                     TDPQESDSIGVRHIPMGVPLQTEMHAYMEILKKYPPRAQIKSD"
     gene            complement(1794..3029)
                     /locus_tag="J1Y83_001311"
     CDS             complement(1794..3029)
                     /locus_tag="J1Y83_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfatase maturase"
                     /protein_id="HBA7629476.1"
                     /translation="MQQQVPTRAFHVMAKPSGSDCNLNCDYCFYLEKQSLYHEKPVTH
                     MDDDTLEAYVRHYIAASETQNEVAFTWQGGEPTLLGLDFYRRAVALQAKYGAGRKISN
                     SFQTNGVLLDDEWCAFLAENHFLVGLSLDGPAEIHNQYRVTKGGRPTHKLVMRALTLL
                     QKHHVDYNVLVCVNRTSALQPLQVYDFLCDAGVEFIQFIPVVERLADETAAHAGLKLH
                     APGDIQGELTEWSVCPQEFGEFLVAIFDHWIKRDVGKIFVMNIEWAFANFVGAPGAVC
                     HHQPTCGRSVIVEHNGDVYACDHYVYPQYRLGNMLQQTIAEMIDSPQQQAFGEDKFKQ
                     LPAQCRSCNVLKACWGGCPKHRFMLDASGKPGLNYLCAGYQRYFRHLPPYLKAMVDLL
                     AHGRPASDIMQAHLLVVNK"
     gene            complement(3176..3252)
                     /locus_tag="J1Y83_001312"
     tRNA            complement(3176..3252)
                     /locus_tag="J1Y83_001312"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3216..3218),aa:Pro,seq:tgg)
     gene            complement(3295..3381)
                     /locus_tag="J1Y83_001313"
     tRNA            complement(3295..3381)
                     /locus_tag="J1Y83_001313"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3345..3347),aa:Leu,seq:cag)
     gene            complement(3402..3477)
                     /locus_tag="J1Y83_001314"
     tRNA            complement(3402..3477)
                     /locus_tag="J1Y83_001314"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3442..3444),aa:His,seq:gtg)
     gene            complement(3536..3612)
                     /locus_tag="J1Y83_001315"
     tRNA            complement(3536..3612)
                     /locus_tag="J1Y83_001315"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3576..3578),aa:Arg,seq:ccg)
     gene            complement(3715..5100)
                     /locus_tag="J1Y83_001316"
     CDS             complement(3715..5100)
                     /locus_tag="J1Y83_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBA7629477.1"
                     /translation="MADNKPELQRGLEARHIELIALGGTIGVGLFMGAASTLKWAGPS
                     VLLAYIIAGLFVFFIMRSMGEMLFLEPVTGSFAVYAHRYMSPFFGYLTAWSYWFMWMA
                     VGISEITAIGVYVQFWFPEMAQWIPALIAVALVALANLAAVRLYGEIEFWFAMIKVTT
                     IIVMIVIGLGVIFFGFGNGGQSIGFSNLTEHGGFFAGGWKGFLTALCIVVASYQGVEL
                     IGITAGEAKNPQVTLRSAVGKVLWRILIFYVGAIFVIVTIFPWNEIGSNGSPFVLTFA
                     KIGITAAAGIINFVVLTAALSGCNSGMYSCGRMLYALAKNRQLPAAMAKVSRHGVPVA
                     GVAVSIAILLIGSCLNYIIPNPQRVFVYVYSASVLPGMVPWFVILISQLRFRRAHKAA
                     IASHPFRSILFPWANYVTMAFLICVLIGMYFNEDTRMSLFVGIIFMLVVTAIYKVFGL
                     NRHGKAHKLEE"
     gene            complement(5291..6031)
                     /gene="rffM"
                     /locus_tag="J1Y83_001317"
     CDS             complement(5291..6031)
                     /gene="rffM"
                     /locus_tag="J1Y83_001317"
                     /EC_number="2.4.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide
                     N-acetylmannosaminouronosyltransferase"
                     /protein_id="HBA7629478.1"
                     /translation="MNNNTTAPTYTLRGLQLIGWRDMQHALDYLFADGQLKQGTLVAI
                     NAEKMLTIEDNAEVRELINAAEFKYADGISVVRSVRKKYPQAQVSRVAGADLWEELMA
                     RAGKEGTPVFLVGGKPEVLAQTEAKLRNQWNVNIVGSQDGYFKPEQRQALFERIHASG
                     AQIVTVAMGSPKQEIFMRDCRLVHPDALYMGVGGTYDVFTGHVKRAPKIWQTLGLEWL
                     YRLLSQPSRIKRQLRLLRYLRWHYTGNL"
     gene            complement(6034..7386)
                     /gene="wzyE"
                     /locus_tag="J1Y83_001318"
     CDS             complement(6034..7386)
                     /gene="wzyE"
                     /locus_tag="J1Y83_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen assembly polymerase"
                     /protein_id="HBA7629479.1"
                     /translation="MSLLQFSGLFVVWLLCTLFIATLTWFEFRRVRFNFNVFFSLLFL
                     LTFFFGFPLTSVLVFRFDVGVAPPEILLQALLSAGCFYAVYYVTYKTRLRKRVADAPR
                     RPLFTMNRVETNLTWVILMGIALVSVGIFFMHNGFLLFRLNSYSQIFSSEVSGVALKR
                     FFYFFIPAMLVVYFLRQDSKAWLFFLVSTVAFGLLTYMIVGGTRANIIIAFAIFLFIG
                     IIRGWISLWMLAAAGVLGIVGMFWLALKRYGMNVSGDEAFYTFLYLTRDTFSPWENLA
                     LLLQNYDNIDFQGLAPIVRDFYVFIPSWLWPGRPSMVLNSANYFTWEVLNNHSGLAIS
                     PTLIGSLVVMGGALFIPLGAIVVGLIIKWFDWLYELGNRETNRYKAAILHSFCFGAIF
                     NMIVLAREGLDSFVSRVVFFIVVFGACLMIAKLLYWLFESAGLIHKRTKSSLRTQVEG
                     "
     gene            complement(7383..8462)
                     /gene="rffT"
                     /locus_tag="J1Y83_001319"
                     /gene_synonym="wecF"
     CDS             complement(7383..8462)
                     /gene="rffT"
                     /locus_tag="J1Y83_001319"
                     /gene_synonym="wecF"
                     /EC_number="2.4.1.325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDP-N-acetylfucosamine:lipid II
                     N-acetylfucosaminyltransferase"
                     /protein_id="HBA7629480.1"
                     /translation="MTVLIHVLGSDIPHHNRTVLRFFNDALAATSEHAREFMVAGKDD
                     GLSDSCLALSVQFFPGKKSLAEAVIAKAKANRQQRFFFHGQFNPKLWLALLSGGIKPS
                     QFFWHIWGADLYELSSGLRYKLFYPLRRLAQKRVGCVFATRGDLSFFAKTHPKVRGEL
                     LYFPTRMDPSLNTMANDRQREGKMTILVGNSGDRSNEHIAALRAVHQQFGDTVKVVVP
                     MGYPPNNEAYIEEVRQAGLELFSEENLQVLSEKLEFDAYLTLLRQCDLGYFIFARQQG
                     IGTLCLLIQAGIPCVLNRENPFWQDMTEQHLPVLFTTDDLNEDIVREAQRQLASVDKN
                     TIAFFSPNYLQGWQRALAIAAGEVA"
     gene            complement(8459..9709)
                     /gene="wzxE"
                     /locus_tag="J1Y83_001320"
     CDS             complement(8459..9709)
                     /gene="wzxE"
                     /locus_tag="J1Y83_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid III flippase WzxE"
                     /protein_id="HBA7629481.1"
                     /translation="MSLAKASLWTAASTLVKIGAGLLVGKLLAVSFGPAGLGLAANFR
                     QLITVLGVLAGAGIFNGVTKYVAQYHDNPQQLRRVVGTSSAMVLGFSTLMALVFVLAA
                     APISQGLFGNTDYQGLVRLVALVQMGIAWGNLLLALMKGFRDAAGNALSLIVGSLIGV
                     LAYYVSYRLGGYEGALLGLALIPALVVIPAVIMLIKRGVIPLSYLKPSWDNGLAGQLS
                     KFTLMALITSVTLPVAYIMMRKLLAAQYSWDEVGIWQGVSSISDAYLQFITASFSVYL
                     LPTLSRLTEKRDITREVVKSLKFVLPAVAAASFTVWLLRDFAIWLLLSNKFTAMRDLF
                     AWQLVGDVLKVGAYVFGYLVIAKASLRFYILAEISQFTLLMVFAHWLIPAHGALGAAQ
                     AYMATYIVYFSLCCGVFLLWRRRA"
     gene            complement(9711..10841)
                     /gene="rffA"
                     /locus_tag="J1Y83_001321"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
     CDS             complement(9711..10841)
                     /gene="rffA"
                     /locus_tag="J1Y83_001321"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
                     /EC_number="2.6.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-amino-4,6-dideoxygalactose transaminase"
                     /protein_id="HBA7629482.1"
                     /translation="MIPFNAPPVVGTELDYMQSAMGSGKLCGDGGFTRRCQQWLEQRF
                     GSAKVLLTPSCTASLEMAALLLDIQPGDEVIMPSYTFVSTANAFVLRGAKIVFVDVRP
                     DTMNIDETLIEAAITDKTRVIVPVHYAGVACEMDTIMALAKKHNLFVVEDAAQGVMST
                     YKGRALGTIGHIGCFSFHETKNYTAGGEGGATLINDKALIERAEIIREKGTNRSQFFR
                     GQVDKYTWRDIGSSYLMSDLQAAYLWAQLEAAERINQQRLALWQNYYDALAPLAKAGR
                     IELPSIPDGCVQNAHMFYIKLRDIDDRSALINFLKEAEIMAVFHYIPLHGCPAGERFG
                     EFHGEDRYTTKESERLLRLPLFYNLSPVNQRTVIATLLNYFS"
     gene            complement(10846..11520)
                     /locus_tag="J1Y83_001322"
     CDS             complement(10846..11520)
                     /locus_tag="J1Y83_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-fucosamine acetyltransferase"
                     /protein_id="HBA7629483.1"
                     /translation="MPVRASIEPLTWENAFFGVNSAIVRITSEAPLLTPDALAPWSRV
                     QAKIAASNTGELDALQQLGFSLVEGEVDLALPVNNVSDSGAVVAQETDIPALRQLASA
                     AFAQSRFRAPWYAPDASGRFYAQWIENAVRGTFDHQCLILRAASGDIRGYVSLRELNA
                     TDARIGLLAGRGAGAELMQTALNWAYARGKTTLRVATQMGNTAALKRYIQSGANVEST
                     AYWLYR"
     gene            complement(11498..12379)
                     /gene="rfbA"
                     /locus_tag="J1Y83_001323"
     CDS             complement(11498..12379)
                     /gene="rfbA"
                     /locus_tag="J1Y83_001323"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HBA7629484.1"
                     /translation="MKGIILAGGSGTRLHPITRGVSKQLLPIYDKPMIYYPLSVLMLA
                     GIREILIITTPEDKGYFQRLLGDGSEFGIQLEYAEQPSPDGLAQAFIIGETFLNGEPS
                     CLVLGDNIFFGQGFSPKLRHVAARTEGATVFGYQVMDPERFGVVEFDDNFRAISLEEK
                     PKQPKSNWAVTGLYFYDSKVVEYAKQVKPSERGELEITSINQMYLEAGNLTVELLGRG
                     FAWLDTGTHDSLIEASTFVQTVEKRQGFKIACLEEIAWRNGWLDDEGVKRAASSLAKT
                     GYGQYLLELLRARPRQY"
     gene            complement(12398..13465)
                     /gene="rffG"
                     /locus_tag="J1Y83_001324"
     CDS             complement(12398..13465)
                     /gene="rffG"
                     /locus_tag="J1Y83_001324"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HBA7629485.1"
                     /translation="MRKILITGGAGFIGSALVRYIINETSDAVVVVDKLTYAGNLMSL
                     APVAQSERFAFEKVDICDRAELARVFTEYQPDCVMHLAAESHVDRSIDGPAAFIETNI
                     VGTYTLLEAARAYWTALTEDKKSAFRFHHISTDEVYGDLHSTDDFFTETTPYAPSSPY
                     SASKASSDHLVRAWLRTYGLPTLITNCSNNYGPYHFPEKLIPLMILNALAGKPLPVYG
                     NGQQIRDWLYVEDHARALYCVATTGKVGETYNIGGHNERKNLDVVETICELLEELAPN
                     KPHGVAHYRDLITFVADRPGHDLRYAIDASKIARELGWLPQETFESGMRKTVQWYLGN
                     EIWWKQVQDGSYQGERLGLKG"
     gene            complement(13465..14727)
                     /gene="wecC"
                     /locus_tag="J1Y83_001325"
     CDS             complement(13465..14727)
                     /gene="wecC"
                     /locus_tag="J1Y83_001325"
                     /EC_number="1.1.1.336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetyl-D-mannosamine dehydrogenase"
                     /protein_id="HBA7629486.1"
                     /translation="MSFATISVIGLGYIGLPTAAAFASRQKQVIGVDINQHAVDTINR
                     GEIHIVEPDLASVVKTAVEGGFLRASTTPVEADAWLIAVPTPFKGDHEPDMTYVESAA
                     RSIAPVLKKGALVILESTSPVGSTEKMAEWLAEMRPDLTFPQQVGELADVNIAYCPER
                     VLPGQVMVELIKNDRVIGGMTPVCSARASELYKIFLEGECVVTNSRTAEMCKLTENSF
                     RDVNIAFANELSLICADQGINVWELIRLANRHPRVNILQPGPGVGGHCIAVDPWFIVA
                     QNPQQARLIRTAREVNDHKPFWVIDQVKAAVADCLAATDKRASELKIACFGLAFKPNI
                     DDLRESPAMEIAELIAQWHSGETLVVEPNIHELPKKLTGLCTLAQLDEALATADVLVM
                     LVDHSQFKVINGDNVHQQYVVDAKGVWR"
     gene            complement(14724..15854)
                     /gene="wecB"
                     /locus_tag="J1Y83_001326"
     CDS             complement(14724..15854)
                     /gene="wecB"
                     /locus_tag="J1Y83_001326"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HBA7629487.1"
                     /translation="MKVLTVFGTRPEAIKMAPLVHALAKDPFFEAKVCVTAQHREMLD
                     QVLKLFSIVPDYDLNIMQPGQGLTEITCRILEGLKPILAEFKPDVVLVHGDTTTTLAT
                     SLAAFYQRIPVGHVEAGLRTGDLYSPWPEEANRTLTGHLAMYHFSPTETSRQNLLREN
                     VADSRIFITGNTVIDALLWVRDQVMSSDTLRSELAANYPFIDPDKKMILVTGHRRESF
                     GRGFEEICHALADIATTHQDIQIVYPVHLNPNVREPVNRILGHVKNVILIDPQEYLPF
                     VWLMNHAWLILTDSGGIQEEAPSLGKPVLVMRDTTERPEAVTAGTVRLVGTDKQRIVE
                     EVTRLLKDENEYQAMSRAHNPYGDGQACSRILEALKNNRISL"
     gene            complement(15910..16956)
                     /gene="wzzE"
                     /locus_tag="J1Y83_001327"
     CDS             complement(15910..16956)
                     /gene="wzzE"
                     /locus_tag="J1Y83_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECA polysaccharide chain length modulation
                     protein"
                     /protein_id="HBA7629488.1"
                     /translation="MTQPMPGKPAEDAENELDIRGLFRTLWAGKLWIIGMGLAFALIA
                     LAYTFFARQEWSSTAITDRPTVNMLGGYYSQQQFLRNLDVRSNMASADQPSVMDEAYK
                     EFVMQLASWDTRREFWLQTDYYKQRMVGNSKADAALLDEMINNIVFIPGDFTRAVNDS
                     VKLIAETAPDANNLLRQYVAFASQRAASHLNDELKGAWAARTIQMKAQVKRQEEVAKA
                     IYDRRMNSIEQALKIAEQHNISRSATDVPAEELPDSEMFLLGRPMLQARLENLQAVGP
                     AFDLDYDQNRAMLNTLNVGPTLDPRFQTYRYLRTPEEPVKRDSPRRAFMMIMWGIVGG
                     LIGAGVALTRRCSK"
     gene            complement(16968..18071)
                     /gene="wecA"
                     /locus_tag="J1Y83_001328"
     CDS             complement(16968..18071)
                     /gene="wecA"
                     /locus_tag="J1Y83_001328"
                     /EC_number="2.7.8.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine--undecaprenyl-phosphate
                     N-acetylglucosaminephosphotransferase"
                     /protein_id="HBA7629489.1"
                     /translation="MNLLTVSTDLISIFLFTTLFLFFARKVAKKVGLVDKPNFRKRHQ
                     GLIPLVGGISVYAGICFTFGIVDYYIPHASLYLACAGVLVFIGALDDRFDISVKIRAT
                     IQAAVGIVMMVFGKLYLSSLGYIFGSWEMVLGPFGYFLTLFAVWAAINAFNMVDGIDG
                     LLGGLSCVSFAAIGMILWFDGQTSLAIWCFAMIAAILPYIMLNLGILGRRYKVFMGDA
                     GSTLIGFTVIWILLETTQGKTHPISPVTALWIIAIPLMDMVAIMYRRLRKGMSPFSPD
                     RQHIHHLIMRAGFTSRQAFVLITLAAALLASIGVLAEYSHFVPEWVMLVLFLLAFFLY
                     GYCIKRAWKVARFIKRVKRRLRRNRGGSPNLTK"
     gene            complement(18311..19570)
                     /gene="rho"
                     /locus_tag="J1Y83_001329"
     CDS             complement(18311..19570)
                     /gene="rho"
                     /locus_tag="J1Y83_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HBA7629490.1"
                     /translation="MNLTELKNTPVSELITLGENMGLENLARMRKQDIIFAILKQHAK
                     SGEDIFGDGVLEILQDGFGFLRSADSSYLAGPDDIYVSPSQIRRFNLRTGDTISGKIR
                     PPKEGERYFALLKVNEVNFDKPENARNKILFENLTPLHANSRLRMERGNGSTEDLTAR
                     VLDLASPIGRGQRGLIVAPPKAGKTMLLQNIAQSIAYNHPDCVLMVLLIDERPEEVTE
                     MQRLVKGEVVASTFDEPASRHVQVAEMVIEKAKRLVEHKKDVIILLDSITRLARAYNT
                     VVPASGKVLTGGVDANALHRPKRFFGAARNVEEGGSLTIIATALIDTGSKMDEVIYEE
                     FKGTGNMELHLSRKIAEKRVFPAIDYNRSGTRKEELLTTQEELQKMWILRKIIHPMGE
                     IDAMEFLINKLAMTKTNDDFFEMMKRS"
     gene            complement(19655..19756)
                     /gene="rhoL"
                     /locus_tag="J1Y83_001330"
     CDS             complement(19655..19756)
                     /gene="rhoL"
                     /locus_tag="J1Y83_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rho operon leader peptide rhoL"
                     /protein_id="HBA7629491.1"
                     /translation="MRSEQISGSSLNPSCRFSSAYSPVTRQRKDMSR"
     gene            complement(19897..20226)
                     /gene="trxA"
                     /locus_tag="J1Y83_001331"
     CDS             complement(19897..20226)
                     /gene="trxA"
                     /locus_tag="J1Y83_001331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017375425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxA"
                     /protein_id="HBA7629492.1"
                     /translation="MSDKIIHLTDDSFDTDVLKADGAILVDFWAEWCGPCKMIAPILD
                     EIADEYQGKLTVAKLNIDQNPGTAPKYGIRGIPTLLLFKNGEVAATKVGALSKGQLKE
                     FLDANLA"
     gene            20357..21622
                     /gene="rhlB"
                     /locus_tag="J1Y83_001332"
     CDS             20357..21622
                     /gene="rhlB"
                     /locus_tag="J1Y83_001332"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase RhlB"
                     /protein_id="HBA7629493.1"
                     /translation="MSKTHLTEQKFSDFALHPKVVEALEKKGFHNCTPIQALALPLTL
                     AGRDVAGQAQTGTGKTMAFLTSTFHYLLSHPAIADRKVNQPRALIMAPTRELAVQIHA
                     DAEPLAEATGLKLGLAYGGDGYDKQLKVLESGVDILIGTTGRLIDYAKQNHINLGAIQ
                     VVVLDEADRMYDLGFIKDIRWLFRRMPPANQRLNMLFSATLSYRVRELAFEQMNNAEY
                     IEVEPEQKTGHRIKEELFYPSNEEKMRLLQTLIEEEWPDRAIIFANTKHRCEEIWGHL
                     AADGHRVGLLTGDVAQKKRLRILDEFTRGDLDILVATDVAARGLHIPAVTHVFNYDLP
                     DDCEDYVHRIGRTGRAGASGHSISLACEEYALNLPAIETYIGHSIPVSKYNPDALMTD
                     LPKPLRLTRPRTGNGPRRTGAPRNRRRSG"
     gene            21758..23242
                     /gene="gppA"
                     /locus_tag="J1Y83_001333"
     CDS             21758..23242
                     /gene="gppA"
                     /locus_tag="J1Y83_001333"
                     /EC_number="3.6.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanosine-5'-triphosphate,3'-diphosphate
                     diphosphatase"
                     /protein_id="HBA7629494.1"
                     /translation="MGSTSSLYAAIDLGSNSFHMLVVREVAGSIQTLTRIKRKVRLAA
                     GLNSENALSNEAMERGWQCLRLFAERLQDIPPSQIRVVATATLRLAVNAGDFIAKAQE
                     ILGCPVQVISGEEEARLIYQGVAHTTGGADQRLVVDIGGASTELVTGTGAQTTSLFSL
                     SMGCVTWLERYFADRNLGQENFDAAEKAAREVLRPVADELRYHGWKVCVGASGTVQAL
                     QEIMMAQGMDERITLEKLQQLKQRAIHCGRLEELEIDGLTLERALVFPSGLAILIAIF
                     TELNIQCMTLAGGALREGLVYGMLHLTVEQDIRSRTLRNIQRRFMIDIDQAQRVAKVA
                     ANFFDQVENEWHLEAISRDLLISACQLHEIGLSVDFKQAPQHAAYLVRNLDLPGFTPA
                     QKKLLATLLLNQTNPVDLSSLHQQNAVPPRVAEQLCRLLRLAIIFASRRRDDLVPEMT
                     LQANHELLTLTLPQGWLTQHPLGKEIIDQESQWQSYVHWPLEVH"
     gene            complement(23289..25310)
                     /gene="rep"
                     /locus_tag="J1Y83_001334"
     CDS             complement(23289..25310)
                     /gene="rep"
                     /locus_tag="J1Y83_001334"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase Rep"
                     /protein_id="HBA7629495.1"
                     /translation="MRLNPGQQQAVEFVTGPCLVLAGAGSGKTRVITNKIAHLIRGCG
                     YQARHIAAVTFTNKAAREMKERVGQTLGRKEARGLMISTFHTLGLDIIKREYAALGMK
                     ANFSLFDDTDQLALLKELTEGLIEDDKVLLQQLISTISNWKNDLKTPAQAAAEAKGER
                     DRIFAHCYGLYDAHLKACNVLDFDDLILLPTLLLQRNEEVRERWQNKIRYLLVDEYQD
                     TNTSQYELVKLLVGSRARFTVVGDDDQSIYSWRGARPQNLVLLSQDFPALKVIKLEQN
                     YRSSGRILKAANILIANNPHVFEKRLFSELGYGTELKVLSANNEEHEAERVTGELIAH
                     HFVNKTQYKDYAILYRGNHQSRVFEKFLMQNRIPYKISGGTSFFSRPEIKDLLAYLRV
                     LTNPDDDSAFLRIVNTPKREIGPATLKKLGEWAMTRNKSMFTASFDMGLSQTLSGRGY
                     EALTRFTHWLAEIQRLAEREPIAAVRDLIHGMDYESWLYETSPSPKAAEMRMKNVNQL
                     FSWMTEMLEGSELDEPMTLTQVVTRFTLRDMMERGESEEELDQVQLMTLHASKGLEFP
                     YVYMVGMEEGFLPHQSSIDEDNIDEERRLAYVGITRAQKELTFTLCKERRQYGELVRP
                     EPSRFLLELPQDDLIWEQERKVVSAEERMQKGQSHLANLKAMMAAKRGK"
     gene            25397..25678
                     /gene="ppiC"
                     /locus_tag="J1Y83_001335"
     CDS             25397..25678
                     /gene="ppiC"
                     /locus_tag="J1Y83_001335"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004097481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase PpiC"
                     /protein_id="HBA7629496.1"
                     /translation="MAKTAAALHILVKEEKLALDLLEQIKNGADFGKLAKKHSICPSG
                     KRGGDLGEFRQGQMVPAFDKVVFSCPVLEPTGPLHTQFGYHIIKVLYRN"
     gene            complement(25724..27199)
                     /gene="ilvC"
                     /locus_tag="J1Y83_001336"
     CDS             complement(25724..27199)
                     /gene="ilvC"
                     /locus_tag="J1Y83_001336"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HBA7629497.1"
                     /translation="MANYFNTLNLRQQLAQLGKCRFMGRDEFADGASYLQGKKVVIVG
                     CGAQGLNQGLNMRDSGLDISYALRKEAIAEKRASWRKATENGFKVGTYEELIPQADLV
                     VNLTPDKQHSDVVRSVQPLMKDGAALGYSHGFNIVEVGEQIRKDITVVMVAPKCPGTE
                     VREEYKRGFGVPTLIAVHPENDPKGEGMAIAKAWAAATGGHRAGVLESSFVAEVKSDL
                     MGEQTILCGMLQAGSLLCFDKLVEEGTDPAYAEKLIQFGWETITEALKQGGITLMMDR
                     LSNPAKLRAYALSEQLKEIMAPLFQKHMDDIISGEFSSGMMADWANDDKKLLTWREET
                     GKTAFETAPQYEGKIGEQEYFDKGVLMIAMVKAGVELAFETMVDSGIIEESAYYESLH
                     ELPLIANTIARKRLYEMNVVISDTAEYGNYLFSYACVPLLKPFMAELQPGDLGKAIPE
                     GAVDNAQLRDVNEAIRSHAIEQVGKKLRGYMTDMKRIAVAG"
     gene            27349..28239
                     /gene="ilvY"
                     /locus_tag="J1Y83_001337"
     CDS             27349..28239
                     /gene="ilvY"
                     /locus_tag="J1Y83_001337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator IlvY"
                     /protein_id="HBA7629498.1"
                     /translation="MDLRDLKTFLHLAESRHFGRSARAMHVSPSTLSRQIQRLEDDLG
                     QPLFVRDNRTVTLTEAGEELRVFAQQTLLQYQQLRHTIDQQGPSLSGELHIFCSVTAA
                     YSHLPPILDRFRAEHPSVEIKLTTGDAADAMEKVVTGEADLAIAGKPETLPGAVAFSM
                     LENLAVVLIAPALPCPVRNQVSADKPDWSTVPFIMADQGPVRRRIELWFRRNKISNPM
                     IYATVGGHEAMVSMVALGCGVALLPEVVLENSPEPVRNRVMILERSDEKTPFELGVCV
                     QKKRLHEPLIEAFWKILPNH"
     gene            complement(28236..29780)
                     /gene="ilvA"
                     /locus_tag="J1Y83_001338"
     CDS             complement(28236..29780)
                     /gene="ilvA"
                     /locus_tag="J1Y83_001338"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase, biosynthetic"
                     /protein_id="HBA7629499.1"
                     /translation="MADSQPLSGAPEGAEYLRAVLRAPVYEAAQVTPLQKMEKLSSRL
                     DNVILVKREDRQPVHSFKLRGAYAMMAGLTEEQKAHGVITASAGNHAQGVAFSSARLG
                     VKALIVMPTATADIKVDAVRGFGGEVLLHGANFDEAKAKAIELSQQQGFTWVPPFDHP
                     MVIAGQGTLALELLQQDAHLDRVFVPVGGGGLAAGVAVLIKQLMPQIKVIAVEAEDSA
                     CLKAALNAGHPVDLPRVGLFAEGVAVKRIGDETFRLCQEYLDDIITVDSDAICAAMKD
                     LFEDVRAVAEPSGALALAGMKKYIAQHNIRGERLAHILSGANVNFHGLRYVSERCELG
                     EQREALLAVTIPEEKGSFLKFCQLLGGRSVTEFNYRFADAKNACIFVGVRLSRGLEER
                     KEILQMLNDGGYSVVDLSDDEMAKLHVRYMVGGRPSHPLQERLYSFEFPESPGALLRF
                     LNTLGTHWNISLFHYRSHGTDYGRVLAAFELGDHEPDFETRLNELGYDCHDETNNPAF
                     RFFLAG"
     gene            complement(29783..31633)
                     /gene="ilvD"
                     /locus_tag="J1Y83_001339"
     CDS             complement(29783..31633)
                     /gene="ilvD"
                     /locus_tag="J1Y83_001339"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HBA7629500.1"
                     /translation="MPKYRSATTTHGRNMAGARALWRATGMTDADFGKPIIAVVNSFT
                     QFVPGHVHLRDLGKLVAEQIEAAGGVAKEFNTIAVDDGIAMGHGGMLYSLPSRELIAD
                     SVEYMVNAHCADAMVCISNCDKITPGMLMASLRLNIPVIFVSGGPMEAGKTKLSDQII
                     KLDLVDAMIQGADPKVSDSQSDQVERSACPTCGSCSGMFTANSMNCLTEALGLSQPGN
                     GSLLATHADRKQLFLNAGKRIVELTKRYYEQDDESALPRNIASKAAFENAMTLDIAMG
                     GSTNTVLHLLAAAQEAEIDFTMSDIDKLSRKVPQLCKVAPSTQKYHMEDVHRAGGVIG
                     ILGELDRAGLLNRDVKNVLGLTLPQTLEQYDIIVTQDDAVKNMFRAGPAGIRTTQAFS
                     QDCRWDTLDDDRSNGCIRSLEHAYSKDGGLAVLYGNFAENGCIVKTAGVDDSILKFTG
                     PAKVYESQDDAVEAILGGKVVAGDVVVIRYEGPKGGPGMQEMLYPTSFLKSMGLGKAC
                     ALITDGRFSGGTSGLSIGHVSPEAASGGSIGLIEDGDLIAIDIPNRGIQLQVSDAELA
                     ARREAQEARGNKAWTPKNRERQVSFALRAYASLATSADKGAVRDKSKLGG"
     gene            complement(31698..32627)
                     /gene="ilvE"
                     /locus_tag="J1Y83_001340"
     CDS             complement(31698..32627)
                     /gene="ilvE"
                     /locus_tag="J1Y83_001340"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain-amino-acid transaminase"
                     /protein_id="HBA7629501.1"
                     /translation="MTTKKADYIWFNGEMVRWEDAKVHVMSHALHYGTSVFEGIRCYD
                     SHKGPVVFRHREHMQRLHDSAKIYRFPVSQSIDELMEACRDVIRKNNLTSAYIRPLIF
                     VGDVGMGVNPPAGYSTDVIIAAFPWGAYLGAEALEQGIDAMVSSWNRAAPNTIPTAAK
                     AGGNYLSSLLVGSEARRHGYQEGIALDVNGYISEGAGENLFEVKDGVLFTPPFTSSAL
                     PGITRDAIIKLAKELGIEVREQVLSRESLYLADEVFMSGTAAEITPVRSVDGIQVGEG
                     RCGPVTKRIQQAFFGLFTGETEDKWGWLDQVNQ"
     gene            complement(32647..32910)
                     /gene="ilvM"
                     /locus_tag="J1Y83_001341"
     CDS             complement(32647..32910)
                     /gene="ilvM"
                     /locus_tag="J1Y83_001341"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 2 small subunit"
                     /protein_id="HBA7629502.1"
                     /translation="MMQHQVNVSARFNPETLERVLRVVRHRGFHVCSMNMAAASDAQN
                     INIELTVASPRSVDLLFSQLNKLVDVAHVAICQSTTTSQQIRA"
     gene            complement(32907..34553)
                     /locus_tag="J1Y83_001342"
     CDS             complement(32907..34553)
                     /locus_tag="J1Y83_001342"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001012636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 2 catalytic subunit"
                     /protein_id="HBA7629503.1"
                     /translation="MNGAQWVVHALRAQGVNTVFGYPGGAIMPVYDALYDGGVEHLLC
                     RHEQGAAMAAIGYARATGKTGVCIATSGPGATNLITGLADALLDSIPVVAITGQVSAP
                     FIGTDAFQEVDILGLSLACTKHSFLVQSLEELPRIMAEAFDVASSGRPGPVLVDIPKD
                     IQLASGDLEPWFTTVENEVTFSHAEVEQARQMLAKAQKPMLYVGGGVGMAQAVSALRE
                     FLAATKMPATCTLKGLGAVEADYPYYLGMLGMHGTKAANFAVQECDLLIAVGARFDDR
                     VTGKLNTFAPHASVIHMDIDPAEMNKLRQAHVALQGDLNALLPALQQPLNINDWQQYC
                     AQLRDEHTWRYDHPGDAIYAPLLLKQLSDRKPADCVVTTDVGQHQMWAAQHIAHTRPE
                     NFITSSGLGTMGFGLPAAVGAQVARPNDTVVCISGDGSFMMNVQELGTVKRKQLPLKI
                     VLLDNQRLGMVRQWQQLFFQERYSETTLTDNPDFLMLASAFGIPGQHITRKDQVEAAL
                     DTMLNSDGPYLLHVSIDELENVWPLVPPGASNSEMLEKLS"
     gene            complement(34556..34606)
                     /locus_tag="J1Y83_001343"
     CDS             complement(34556..34606)
                     /locus_tag="J1Y83_001343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA7629504.1"
                     /translation="MNNSTKFCFSRFRTGN"
     gene            complement(34693..34791)
                     /gene="ilvL"
                     /locus_tag="J1Y83_001344"
     CDS             complement(34693..34791)
                     /gene="ilvL"
                     /locus_tag="J1Y83_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ilv operon leader peptide"
                     /protein_id="HBA7629505.1"
                     /translation="MTALLRVISLVVISVVVIIIPPCGAALGRGKA"
BASE COUNT         8426 a         9638 c         8832 g         7956 t
ORIGIN      
        1 gcttaattac gtcatcattt tgacgacatg aaaccggagc attttgcatg gaattttcgt
       61 tttcacccaa acttcttgtt gttgctgtcg ccgcagctct tcctctcata gccagcgcag
      121 cagatacccc gtcaaccgcc accgcacgca aaggctttgc cggatacgat cacccaaacc
      181 agtatctggt taaaccggcg accactattg ccgacaacat gatgccggtg atgcagcatc
      241 ctgcacaaga caaagaaacc cagcagaaac tggcggaact tgagaaaaaa accggtaaga
      301 aaccgaatgt ggttgttttc ttgctggatg atgtgggctg gatggacgtt ggctttaacg
      361 gcggcggcgt ggcggtgggt aaccctacac cagatatcga cgccgttgcc agccaggggc
      421 tgattttaac ttcggcgtat tctcaaccga gttcttcccc gacccgcgcc accattctca
      481 ccggacaata ctccatccac cacggcattc tgatgccgcc aatgtacggg caaccgggcg
      541 gtctgcaagg gttaaccacg ctgccgcagt tgctgcacga tcagggctac gtcactcagg
      601 ccatcgggaa atggcatatg ggggaaaaca aagagtcgca gccgcagaac gttggctttg
      661 atgatttccg cggctttaac tcggtatccg atatgtatac cgaatggcgt gatgttcacg
      721 tcaacccgga agtcgccctg agcccggatc gttctgaata catccaaaaa ctgccattca
      781 gcaaagatga cgttcatgcc gtgcgcggcg gtgaacagga agccattgcc gacattacgc
      841 cgaaatatat ggaagacctg gatcaacgct ggatggaata tggcgttaag ttcctcgaca
      901 agatggcgaa gagcgataag cctttcttcc tctactacgg cactcgcggc tgtcacttcg
      961 ataactaccc aaatgcgaaa tatgcgggta gctctccggc gcgcacctct tacggcgact
     1021 gcatggtgga gatgaacgat atcttcgcca atctgtataa agcactggag aaaaacggtc
     1081 agcttgataa cacgctgatt gtgttcacct ctgataacgg tccggaagcc gaagttccgc
     1141 cgcacggtcg cacgccgttc cgcggtgcga aaggttctac atgggaaggc ggcgttcgtg
     1201 tgccaacctt cgtttactgg aaaggcatga tccaaccgcg taaatctgac ggcattgtcg
     1261 atctggcgga tctcttccct acggcgctgg atctggcggg gcatcctggg gcgaaagtag
     1321 cgaatttagt accgaaaacc acctttatcg atggtgtgga ccagacatct ttcttcctgg
     1381 gaacaaatgg tcagtctaac cgtaaggccg agcattactt cctcaacggt aaactttctg
     1441 ctgtgcgtat ggatgagttc aagtatcacg tcctgattca gcaaccttac gcttataccc
     1501 agagcggata tcagggtgga ttcaccggca cagtaatgca aacggcggga tcgtcggtgt
     1561 ttaacctgta caccgatccg caggaaagcg actctatcgg cgtgcgccat atcccaatgg
     1621 gtgtaccgct acagaccgaa atgcatgcgt atatggagat cctgaaaaaa tatccaccac
     1681 gcgcgcagat taaatctgat taagccggcg ctatcgctga gatctacctt tgccggatgc
     1741 gatgctgacg catccgacaa tcaaccgcag gcggccacaa aatggctgct tttttactta
     1801 ttcaccacca gcaaatgcgc ctgcataatg tcgctggccg gacgaccgtg cgccagcaaa
     1861 tcaaccattg ctttaagata tggcggtaga tggcgaaaat aacgctgata cccggcacac
     1921 aaataattca gcccgggttt gccgctggca tcgagcataa agcggtgttt cgggcagcct
     1981 ccccagcacg cttttaacac gttgcaactg cgacactgtg ccggtaactg cttgaattta
     2041 tcttcaccaa atgcctgctg ttgcggggaa tcaatcattt ctgcaatcgt ctgctgaagc
     2101 atattcccta gtcgatactg cggataaaca tagtgatcgc aggcataaac gtcaccgttg
     2161 tgctcaacaa tcaccgagcg cccacaggtt ggctgatgat ggcaaaccgc gcccggtgcg
     2221 ccgacaaaat tggcgaacgc ccattcgata ttcattacga aaatcttgcc gacgtcgcgc
     2281 ttaatccagt ggtcgaaaat tgccaccaga aactcaccga actcttgcgg gcacaccgac
     2341 cattccgtca gttcgccctg aatatcgccg ggagcatgta gcttaagtcc agcatgagca
     2401 gccgtttcat cagccaggcg ctcgaccacc ggaataaact ggatgaactc aacacctgca
     2461 tcacacagaa aatcatagac ttgcaaaggt tgtaacgcac tggtgcgatt gacgcagacc
     2521 agcacgttat agtcgacatg atgtttttgc aggagcgtca gagcacgcat caccagctta
     2581 tgcgttggtc tgccaccttt ggtcacgcga tattgattgt ggatctcagc cggaccatcc
     2641 agcgataacc ctacaagaaa atgattttct gccagaaatg cacaccattc gtcatcgagc
     2701 agcacgccgt tagtctggaa gctattactt atcttcctgc cagcaccata tttcgcctgt
     2761 agcgccacgg cacggcggta aaaatccagc ccgagtaacg ttggttcgcc gccctgccag
     2821 gtaaaagcca cttcgttttg ggtttcgctg gcagcgatat agtgacggac atacgcttcc
     2881 agcgtgtcat cgtccatatg cgtgactggc ttttcgtggt aaagggattg tttttcgaga
     2941 taaaaacagt agtcacagtt cagattacaa tcagaaccgc tcggtttcgc catcacatga
     3001 aaagcacgcg ttggaacctg ttgctgcatg gtcgctcctt gcctgaagtc atccttcaat
     3061 aagttaaggg cgaaatcata ccgtggcgca atgcgttatt acgggcgttt attgcgaagg
     3121 gaaagtgacg aaatgcacag aaaacaaaaa accccgccga agcggggttc aaaattggtc
     3181 ggcgagagag gattcgaacc tccgacccac tggtcccaaa ccagttgcgc taccaagctg
     3241 cgctactcgc cgaaatactg ctttttgaat ttttagttca attctttaaa gtcgtggtgc
     3301 gagggggggg acttgaaccc ccacgtccgt aaggacacta acacctgaag ctagcgcgtc
     3361 taccaattcc gccaccttcg cattgtcaca acttctaata atggggtggc taatgggatt
     3421 cgaacccacg acaactggaa tcacaatcca gggctctacc aactgagcta tagccaccac
     3481 tacaaatctt gttacgcggt attactacca ccgcagctca agcgccggga ctaaatggcg
     3541 cgcccgacag gattcgaacc tgagacctct gcctccggag ggcagcgctc tatccagctg
     3601 agctacgggc gcttagcgcc gttgcggggg tggataatac ggacttccca cccctctgtc
     3661 tagtcccttt ttaaataaat tgcgcgtttg gttacggttt gtgcgttttg ctgcttattc
     3721 ctccagttta tgcgctttac cgtggcgatt aagcccaaaa actttataaa tcgccgtcac
     3781 caccagcata aagatgatgc cgacaaacag cgacatacgc gtatcttcat taaagtacat
     3841 gccgatcaaa acgcaaatca ggaatgccat tgttacgtag ttggcccaag ggaacaggat
     3901 tgagcggaac ggatggctgg caattgccgc tttgtgtgca cggcgaaaac gcagttggct
     3961 tatcaaaatc acaaaccatg gcaccatacc cggaagcacg ctggcgctgt agacgtagac
     4021 aaacacacgc tgtggattgg gaatgatgta gttcaggcat gaaccaatta gcagaatagc
     4081 aatagatact gccacacccg caaccggtac gccgtgacgg gaaactttcg ccattgccgc
     4141 cggtaactga cggtttttcg ccagtgcata gagcatacgt ccgcaactgt acatgccgct
     4201 gttacagcca gagagcgcag ccgtcagcac cacaaagttg ataatgcccg ccgctgcggt
     4261 aatgccgatt ttggcgaaag tcagtacgaa cgggctaccg ttgctgccta tttcattcca
     4321 cgggaagata gtaacgataa cgaaaatcgc gccaacgtag aaaatcagga ttcgccacag
     4381 caccttgcct acggcactgc gcagcgtcac ctgcggattc ttcgcttcac cggcagtaat
     4441 gccaatcagc tccacgccct ggtaggacgc caccacaata cacaatgcgg tcaggaaccc
     4501 tttccagcca ccagcaaaga aaccgccatg ctctgtgaga ttgctaaaac caatcgactg
     4561 cccgccattg ccaaagccaa agaaaatcac gcccaggcca atgacaatca tcacgataat
     4621 cgtggtgact ttgatcatcg cgaaccagaa ctctatttcg ccgtacaacc tcaccgcagc
     4681 cagattcgcc aacgccacca gcgccaccgc gatcaatgcg ggtatccact gcgccatctc
     4741 cgggaaccag aactggacat aaacgccaat ggcggtgatt tctgagatcc ccaccgccat
     4801 ccacataaac cagtaagacc aggcggtgag ataaccaaag aacgggctca tataacgatg
     4861 cgcgtaaacg gcgaacgaac cggtaaccgg ttcgaggaac aacatttcgc ccattgaacg
     4921 catgatgaaa aagacgaaca gcccggcgat gatataggcc aacaatacgg atggcccggc
     4981 ccatttcagg gtactggcgg cccccataaa caggccgacg ccaatggtgc cccccagggc
     5041 gatgagttcg atatgtcgag cttccagccc acgctgtagc tctggtttgt tatctgccat
     5101 aaatcctcgt gttgtgtttg catgctttcc ggtgttaccg gttatcgtta tgggtacatc
     5161 gagtgttgcg aatgttttcg taattcagga gaaatggcaa ataaagcatt aaaaatttga
     5221 atgctttgtg taataaaaaa gcagacaggc gacgaagtga ccactccgtc gctttacaaa
     5281 gagaggaaaa tcagaggttg ccggtgtaat gccagcgtaa ataacgcagc aaacgaagct
     5341 gacgcttaat gcggctcggc tgcgaaagca ggcggtagag ccactccagc cccagcgttt
     5401 gccagatttt cggtgcgcgt tttacgtgac cggtgaaaac atcgtaggtc ccgccaacgc
     5461 ccatatacag cgcatctgga tgcaccagac ggcagtcgcg catgaagatc tcctgctttg
     5521 gcgatcccat cgcaacggtg acgatttgcg ctccgctggc atgaatgcgt tcaaacagcg
     5581 cctgacgctg ctcgggttta aaataaccat cctgactgcc aacgatattc acattccact
     5641 ggttgcgcag tttagcttca gtttgcgcca gcacttcagg tttaccgccc acaagaaata
     5701 ccggtgtccc ttctttgcct gcgcgcgcca tcagctcttc ccagagatcg gccccagcaa
     5761 cgcgggaaac ctgcgcctgc gggtactttt tacgtactga acgtacaacg ctgatgccat
     5821 ccgcatattt aaattcggca gcgttaatta actccctgac ctcggcgtta tcttcaatgg
     5881 tcagcatttt ttcggcatta atggcaacca gcgttccctg cttaagctgc ccgtcagcga
     5941 acagataatc gagggcatgc tgcatatcac gccaaccaat caactgtaag ccacgcagcg
     6001 tataggtcgg tgccgtggtg ttgttattca ttgttatcct tcaacctgcg tccggagcga
     6061 tgattttgtt cgtttatgaa tgagtccggc gctttcaaaa agccagtaca acagttttgc
     6121 gatcatcaga catgcgccga agaccacgat aaaaaagacc acgcgtgaga caaacgaatc
     6181 cagcccttca cgcgccagca cgatcatatt gaagatcgcc ccgaagcaga aactgtgcaa
     6241 tatcgcagcc ttatagcgat tagtctcgcg gttgcccagc tcatacagcc agtcgaacca
     6301 tttgatgatc agtccaacaa caatcgcccc gagcgggatg aacaacgcgc cgcccataac
     6361 aaccagtgag cctataagcg taggcgagat cgccagtccg gagtggttat tcagtacttc
     6421 ccaggtaaag tagttggcgg agttcagcac catactcggg cgacccggcc acagccagga
     6481 agggataaag acatagaaat cgcggacaat tggagccagg ccctggaagt cgatgttgtc
     6541 gtagttctgc aacagcaacg ccagattctc ccacggcgag aaggtgtcgc gagtgagata
     6601 gagaaacgta tagaacgctt catcgccgct cacattcatt ccatagcgtt ttagcgccag
     6661 ccagaacatg cccacaatcc ccagcacgcc cgccgccgcc agcatccaca acgaaatcca
     6721 gccgcgaata atgccaataa acaggaagat agcgaatgcg atgatgatat tggcgcgagt
     6781 gccgccgaca atcatgtaag tcaacaagcc aaaggcaacc gtgctgacga ggaaaaacag
     6841 ccacgctttg ctatcctggc gcagaaaata aaccaccagc atcgccggga tgaaaaagta
     6901 aaagaagcgt tttaatgcca cgccggagac ttcgctggaa aagatctgac tgtaagaatt
     6961 aagccggaac agcaaaaagc cgttgtgcat gaagaagatg ccgacgctca ccagagcgat
     7021 gcccatcagg atcacccacg tgagattggt ctccacgcgg ttcatggtaa acagcggacg
     7081 gcgcggtgca tcagcaacgc gtttgcgtag acgggttttg taggtgacat agtaaaccgc
     7141 gtagaagcag cctgcagaaa gcaacgcctg caacaagatt tctggcggcg cgacaccaac
     7201 atcaaagcga aataccagca cgctggtcag cggaaagccg aagaaaaagg tgagcaaaaa
     7261 cagcaatgaa aagaagacat tgaagttaaa gcgcacacgg cgaaactcaa accaggtcag
     7321 cgtggcaata aacagcgtgc agagcagcca gacaacaaac aggccactga attgcagcag
     7381 actcatgcga cctccccggc ggcaatcgcc aacgcccgct gccagccttg tagatagtta
     7441 gggctaaaga aggcaatggt gtttttatcc accgacgcca actggcgctg cgcttcacgc
     7501 acaatatcct cgttgagatc atcggtagta aacagtactg gcaaatgctg ttccgtcata
     7561 tcctgccaga acggattttc ccggttaagc acacaaggaa tgcccgcctg aatcagtaag
     7621 caaagcgtac caatgccctg ctggcgggca aaaataaagt aaccgagatc gcactggcga
     7681 agtagcgtca gataggcgtc aaattccagt ttttcgctca gaacttgtag gttttcttcg
     7741 ctgaataact ccagtcccgc ctgacgaact tcctcgatgt acgcttcgtt attaggcgga
     7801 tatcccatcg gcaccaccac ttttaccgta tcgccaaatt gctgatgaac ggcgcgcaag
     7861 gcagcaatat gctcattgct gcggtcaccg gagttcccca ccagaatggt catttttcct
     7921 tcacgttgcc gatcgttcgc catcgtatta agagaagggt ccatccgcgt cgggaagtac
     7981 agcagttctc cccgcacctt tggatgcgtt ttggcaaaaa agctcaaatc accgcgggtg
     8041 gcaaatacac agccgactct cttttgcgcc aggcgacgta gcgggtaaaa aagcttatat
     8101 ctcaagccac tggaaagctc atacaggtct gctccccaga tatgccagaa aaactggctg
     8161 ggcttaatgc caccactcag cagagccagc cacagtttgg gattgaactg accgtggaaa
     8221 aaaaatcgct gctgacggtt agcttttgct ttcgcgatga ccgcttccgc cagcgatttt
     8281 ttcccaggga aaaattgcac agaaagcgcc agacagctat cacttaagcc gtcgtccttg
     8341 ccagcaacca taaactcgcg cgcgtgctcg ctcgtcgcgg ccagcgcgtc gttgaaaaac
     8401 cgcagaacgg ttcggttatg gtgagggata tccgatccca gtacgtgaat cagtacagtc
     8461 atgcccgcct acgccagagt aaaaacacgc cacaacaaag agaaaaatag acgatatagg
     8521 ttgccatata ggcctgtgct gcgcccagtg cgccatgcgc agggattagc cagtgggcaa
     8581 ataccatcaa taaagtgaac tggctgattt ccgccagaat ataaaaccgc agtgacgctt
     8641 tggcgatcac cagataacca aagacataag cgcccacttt taacacatca cccaccaact
     8701 gccaggcaaa gagatcgcgc atagcggtaa atttattcga caacagcagc cagatagcaa
     8761 aatcacgcag caaccagacg gtaaaactcg ccgccgccac tgccggtaag acgaatttta
     8821 gcgatttaac cacttcccgg gtgatatcgc gcttttccgt tagccgcgac aacgtgggca
     8881 gcaaatatac gctgaacgat gccgtaataa attgcaggta ggcatcggaa atactgctca
     8941 ccccttgcca gatccccact tcatcccagc tatactgcgc cgccagcagt ttacgcatca
     9001 tgatgtaagc aacaggcaag gtcaccgacg taatcaacgc catgagcgta aatttgctca
     9061 actgccctgc cagaccgtta tcccagctgg gtttcagata gcttaacggg atgacaccac
     9121 gtttgatcaa catgataaca gcaggaatta ccaccagcgc gggaatcagc gccagaccca
     9181 gcaacgcccc ttcataaccg cccaaacgat aactgacgta gtacgcgaga acgccaatca
     9241 agctaccgac aatcagagat aacgcattac ctgcggcatc gcgaaagcct ttcatcagcg
     9301 ccagtaacag gttgccccag gcgatcccca tttgcaccag cgccactaaa cgcaccagcc
     9361 cctgatagtc ggtattacca aacaacccct ggctgattgg cgcagctgcc agcacaaaaa
     9421 ccagcgccat tagcgtagag aagccaagta ccatcgctga tgaagtgccg accacgcggc
     9481 gcagttgttg gggattatca tggtactggg caacgtattt ggttacgccg ttaaagatgc
     9541 ctgccccggc aagcacgccg agcacggtaa tcaactggcg gaaatttgcc gccagcccaa
     9601 gccccgccgg accaaatgac actgccagca acttaccgac cagtaacccg gcaccaatct
     9661 tgaccagtgt actggccgcc gtccacaagg acgcttttgc caacgacata tcaggagaag
     9721 tagttcaaca aggtcgcaat taccgtacgc tgattgacgg gcgacaggtt gtagaacaac
     9781 ggcaggcgca gcaggcgctc gctctctttg gtggtgtagc gatcttcacc gtggaactca
     9841 ccaaagcgtt cccccgcagg gcaaccgtgc agcggaatgt aatggaacac cgccatgatt
     9901 tccgcttctt tcagaaagtt aatcaatgcg ctccggtcat caatatcccg cagtttaatg
     9961 tagaacatat gcgcgttctg cacgcagcca tcgggaatcg acggcagctc gatacgcccg
    10021 gctttcgcca gaggtgctaa cgcatcgtag tagttttgcc acagcgccag acgctgctga
    10081 ttgatacgtt cagcggcttc cagttgcgcc cacaggtacg cagcttgcag atcggacatc
    10141 aaatagctgg agccaatatc gcgccaggta tatttatcga cctgaccacg gaagaactgg
    10201 ctgcggtttg tgcctttttc acggatgatc tctgctcgtt cgatcaacgc tttatcgtta
    10261 atcagtgtcg cgccgccttc accacccgcc gtgtagtttt tggtttcatg gaagctaaag
    10321 cagccaatat gaccaatggt tcccagcgca cgccctttgt aagtggacat cacgccctga
    10381 gcggcatctt ccaccacaaa cagattatgc ttcttcgcca acgccataat ggtgtccatt
    10441 tcgcaggcca cacccgcgta atggaccggc acgataacgc gcgttttgtc ggtgatcgcc
    10501 gcttcaatca gcgtttcgtc gatgttcatg gtgtccgggc gaacatccac aaagacgatt
    10561 ttcgcgccac gcagcacaaa ggcattggcg gtggagacaa aggtgtagct cggcatgatc
    10621 acttcatcgc caggctggat atcgagcagc agcgccgcca tctccagcga agcggtacag
    10681 gatggcgtca gtaacacttt ggcgctgcca aaacgttgct ccagccactg ctggcagcga
    10741 cgggtaaaac caccatcgcc gcacagtttg ccgctaccca ttgccgactg catatagtcg
    10801 agttcggttc ccaccaccgg cggtgcgtta aatggaatca tgtgatcacc tgtataacca
    10861 gtacgcggtg ctttctacat tcgcaccact ttgtatgtat cgtttaagcg cggcggtgtt
    10921 gcccatctgg gtcgccaccc gcaaagttgt tttaccgcga gcatacgccc agtttagcgc
    10981 cgtttgcatt agctcagcac ctgcaccgcg tccagccagc aggccaattc gcgcatctgt
    11041 cgcattgagt tcccgtaaag agacatagcc gcgaatatcg ccggacgccg cacgtaaaat
    11101 cagacattga tgatcaaagg tgccgcgcac ggcattttca atccactgtg cataaaagcg
    11161 accgctggcg tcaggcgcat accacggcgc acgaaaacgg ctttgcgcaa atgcggcgct
    11221 ggctaactga cgtaatgcgg gaatatcggt ctcttgtgcc actactgcac cgctatcact
    11281 gacattgttc acgggtagtg ccaaatcaac ttcaccttct accagggaga atcccagctg
    11341 ttgcagagca tccagttcac ccgtatttga tgccgcaatt ttggcctgca cccgtgacca
    11401 cggcgctaac gcgtctggcg tcaggagcgg tgcttcagac gtaatgcgca cgatggcgct
    11461 gttaacacca aagaaggcgt tttcccaggt tagtggctca atactggcgc ggacgggcac
    11521 gaagtaactc cagcagatat tgaccgtagc cagttttcgc taatgaactg gcagcacgct
    11581 tcacaccctc gtcatcgagc cagccgttac gccaggcaat ctcttccagg caggcaatct
    11641 taaagccctg gcgtttttct actgtctgca caaaagtgct ggcctcaatc aggctgtcgt
    11701 gagtgccggt atccagccag gcaaatccgc gcccgagcag ttcaacggtc aggttgcccg
    11761 cctcgaggta catctggttg atggaggtaa tctccagttc accacgctcc gacggcttca
    11821 cctgctttgc gtactccacg actttactgt cgtagaaata aagcccggtc accgcccagt
    11881 ttgatttcgg ctgttttggt ttttcttcca gcgagatagc gcggaaattg tcgtcaaact
    11941 ccaccacgcc aaagcgttcc gggtccatca cctgatagcc aaataccgtc gccccttccg
    12001 tgcgcgccgc aacatgacgc agcttcggac tgaagccctg tccgaagaag atgttatcgc
    12061 ccagcaccag acaagaaggt tcaccgttga ggaaggtttc accgatgata aaggcctgcg
    12121 ccagaccgtc cggactgggc tgttcggcgt attccagctg gataccgaac tcactaccat
    12181 cgcccagcag gcgctggaaa taacctttat cttccggcgt agtgatgatg agaatttcgc
    12241 ggataccggc caacatcagc accgacagcg gatagtaaat cattggctta tcgtaaatcg
    12301 gcaacagttg cttcgatacg ccgcgcgtaa tcggatgcaa tcgggtgccg gaaccgcccg
    12361 ccaggataat acctttcatg tttgcctccg ctgaaaatta gcctttcaga cctaaacgct
    12421 cgccctgata gctgccgtcc tgcacctgct tccaccagat ctcattaccc agataccact
    12481 gcaccgtttt acgcattcca ctttcaaagg tttcctgcgg cagccagcca agttcacggg
    12541 caattttcga agcatcaatg gcataacgca gatcatgccc cggacggtca gcgacaaagg
    12601 tgatcaggtc acgatagtgc gccacgccgt gcggcttatt cggagccagt tcttccagca
    12661 gctcgcaaat ggtttccaca acatcgagat tcttacgctc gttgtgacca ccaatattat
    12721 aggtttcacc gactttcccg gtggtcgcca cgcaatacag cgcgcgggcg tgatcttcaa
    12781 catacagcca gtcacggatt tgctgcccgt tgccatatac cggcagcggt ttacccgcca
    12841 gcgcgttgag gatcatcagc gggatcagtt tttccggaaa gtggtaagga ccgtagttat
    12901 tcgagcagtt ggtgataagc gtcggcagac cataggtccg cagccaggcg cgcaccagat
    12961 ggtcgctgct ggcttttgac gcggaataag ggctactcgg cgcatacggc gtggtttcgg
    13021 tgaagaaatc atccgtcgag tgcaggtcac catatacttc gtcggtggag atatgatgaa
    13081 aacggaacgc tgatttttta tcttccgtca gcgcagtcca gtaagcccgc gccgcttcaa
    13141 gcagtgtata agtcccgaca atgttggttt caataaacgc tgccgggccg tcaatagaac
    13201 ggtcaacatg gctttcggct gccagatgca tgacacagtc tggctgatac tcagtgaata
    13261 cgcgcgccag ttctgcccga tcgcagatat caactttctc aaaggcaaag cgctcgcttt
    13321 gcgcgaccgg tgccagcgac atcagatttc cggcgtaggt cagcttatcg accactacca
    13381 ccgcgtcgct cgtttcgttg atgatataac gcaccagcgc cgagccaata aacccggcac
    13441 cacctgttat cagaattttt ctcatcagcg ccagactcct ttggcatcga cgacatactg
    13501 ctgatggaca ttgtcgccat tgataacttt gaactgacta tgatcgacca gcatcaccag
    13561 cacgtctgcc gttgccagcg cctcgtcaag ctgcgccaga gtacaaagcc cggtcagttt
    13621 cttcggcaac tcgtggatgt taggctcaac aaccagcgtt tcgccgctgt gccactgggc
    13681 gatcagttca gcgatttcca tcgcagggct ttcgcgcagg tcatcaatat tcggtttaaa
    13741 cgccagacca aagcaggcga ttttcagttc actggcgcgt ttatcggtag cagccagaca
    13801 atcagccacc gccgctttca cctgatcgat aacccagaac ggtttgtgat cgttcacttc
    13861 gcgcgcggta cggataagcc gcgcctgctg ggggttctgt gccacgataa accacggatc
    13921 aacagcgatg cagtgaccgc ccacgccagg gcctggctga agaatattga cgcgagggtg
    13981 acgattcgcc aggcgaatca gttcccagac gttaatcccc tgatcggcac aaatcagcga
    14041 caattcatta gcaaaagcga tattcacatc gcggaagctg ttttcggtga gcttacacat
    14101 ttccgccgtc cgcgagttag tgacgacaca ctcaccttcg aggaaaattt tgtacagttc
    14161 gctggcgcgg gccgaacaaa ccggcgtcat accaccaatc acgcgatcgt ttttaatcag
    14221 ctcgaccata acctggcccg gtaatacgcg ttccgggcag taagcaatgt tgacgtccgc
    14281 caactcgccc acctgctgtg ggaaagtgag atctggacgc atctctgcta accattctgc
    14341 catcttctcg gttgacccca ccggcgaggt ggattcaagg atcaccaacg cgcctttttt
    14401 cagcactggc gcaatggagc gagcagccga ttcaacgtag gtcatatctg gctcatgatc
    14461 gcctttaaac ggcgtgggta cagcaatcaa ccaggcatcc gcttcaactg gcgtcgtgct
    14521 cgctcgtaaa aaaccgcctt ctacggcagt ttttactacg ctcgccaaat caggctcgac
    14581 gatatggatt tcgccacgat tgatggtatc aaccgcgtgt tggttgatat cgacaccaat
    14641 cacctgtttt tgccttgagg caaacgcagc tgctgtaggc agcccgatgt aacccagtcc
    14701 gataacagaa atggtcgcaa aactcatagt gatatccgat tattttttaa cgcttccaga
    14761 atgcgagagc atgcctgacc atcaccatac gggttatggg cgcggctcat agcttgatat
    14821 tcgttttcgt cttttaaaag acgcgtcact tcctcgacaa ttcgctgctt atccgtgccg
    14881 accagacgca ccgtccccgc tgtcaccgct tccggacgct cagtggtatc gcgcattacc
    14941 agcaccggtt tccccagcga aggcgcttct tcctgaatgc cgcctgagtc ggtcaaaatc
    15001 agccaggcgt ggttcatcag ccagacaaac ggtaaatact cctggggatc gatcagaatg
    15061 acatttttca cgtgccccag aatgcgattg accggctctc tgacgttcgg gttgagatgc
    15121 accggataga caatctgtat gtcctggtgc gtggtggcga tgtctgccag cgcatggcag
    15181 atttcttcaa agccacgacc gaagctctca cgcctgtgac cggtcaccag aatcatcttt
    15241 ttatcgggat cgataaacgg gtaatttgcc gccagttctg aacgcagcgt atcgctgctc
    15301 atcacctggt cacgcaccca taacagtgca tcaataactg tattaccggt aatgaagatt
    15361 cggctatccg caacgttttc acgcagcaag ttttgccggg aagtttcggt tggagagaag
    15421 tgatacatcg ccagatgccc ggtcaatgta cggttagcct cttccggcca cggcgaatag
    15481 agatcgcccg tgcgcagacc agcctcaacg tgaccaacag gaatacgctg ataaaacgcc
    15541 gccaggctgg ttgccagcgt cgtcgtagta tcgccgtgaa ccagcacgac atctggtttg
    15601 aactcggcaa gaataggttt tagcccttcc agaatccgac aggttatctc tgtaaggccc
    15661 tgtcctggct gcattatgtt gagatcgtag tcaggtacaa tggaaaagag tttcagcacc
    15721 tgatcgagca tctcccgatg ctgcgcagtg acgcaaactt tagcctcaaa aaaaggatct
    15781 tttgccaacg catgcaccaa cggcgccatc ttgatggctt ccgggcgcgt accaaataca
    15841 gtcagtactt tcacatcgat tctcttcgaa taagcggcga gcgcctttgc gctcaccgca
    15901 gcagtgttgc tatttcgagc aacggcgggt taatgcgaca ccagccccga tcagtccccc
    15961 gacaatgccc cacataatca tcatgaaggc acgacgtggg ctatcgcgtt ttaccggttc
    16021 ttccggcgta cgcaaatagc gataggtctg aaaacgcgga tccagggttg gaccaacatt
    16081 cagggtgttt aacatggccc gattctgatc atagtcgaga tcaaaggccg gaccgacggc
    16141 ctgtaaattt tccagtcgag cctggagcat tggacgccca agcaggaaca tttctgaatc
    16201 aggtaattcc tcggcaggca catctgtcgc actgcgcgaa atattatgct gctcagcaat
    16261 tttcagcgcc tgttcaatac tgttcatccg gcgatcgtag atggctttcg ccacctcttc
    16321 ctgacgcttc acctgagctt tcatctggat ggtgcgtgcc gcccatgcgc ctttcagctc
    16381 atcattcaga tggctggctg cacgctggct ggcaaaagca acatactgac gtaacaggtt
    16441 attagcgtca ggcgcagttt cggcaatcag cttcacgctg tcattgaccg cgcgggtgaa
    16501 gtcaccggga ataaacacga tgttgttgat catttcatcc agcaacgccg catctgcttt
    16561 gctgttgccc accatccgct gcttgtaata gtcggtttgc agccagaact ctctgcgggt
    16621 atcccacgag gccagttgca taacaaactc tttataggct tcgtccatga ccgatggttg
    16681 gtcggcagaa gccatgtttg aacggacatc caggttacgc aaaaattgct gctgtgagta
    16741 atatcccccc agcatattca ccgttggacg atcggtaatc gccgtcgagc tccactcctg
    16801 acgagcaaaa aaagtatacg ccagcgcgat taacgcaaac gccagcccca tgccaataat
    16861 ccacagcttc ccagcccaca aggtacgaaa caacccacga atatccagtt cattttcagc
    16921 gtcttcggcc ggtttcccag gcattggttg tgtcatcaca tcctcattta tttggttaaa
    16981 ttggggctgc caccacgatt tctacgcagt ctgcgtttta cgcgcttaat aaagcgagca
    17041 actttccagg cacgcttaat gcaatatcca tagaggaaga atgctagcaa aaagagcacc
    17101 agcatgaccc actccgggac aaaatgagaa tattctgcca gcacgccaat ggaagcgagc
    17161 agtgctgcgg caagggtaat cagcacaaac gcctggcggg aagtaaaccc ggcacgcatg
    17221 atcaaatggt gaatatgctg acggtcagga gagaatgggc tcatgccttt acgcaggcga
    17281 cggtacataa tcgccaccat atccattagc ggaatggcga taatccacaa agcggtaacc
    17341 gggctgatgg gatgagtttt gccctgggtc gtttcgagca ggatccagat cacggtaaaa
    17401 ccaatcagcg tactgcccgc atcccccata aagactttgt agcggcgacc caggatacca
    17461 aggttaagca tgatgtatgg caggatggcg gcgatcatcg caaagcacca gattgcgaga
    17521 ctggtttgcc cgtcgaacca caaaatcata ccgattgctg caaacgagac gcaggacaac
    17581 ccgcccagca agccatcaat gccatcaacc atgttgaacg cattaatggc cgcccagacg
    17641 gcaaatagcg tcaggaagta accaaacggt ccgagcacca tctcccagga gccaaagata
    17701 taacccagac tactgagata aagtttgccg aacaccatca taacaatgcc aacagcggcc
    17761 tgtatggtgg cacggatttt tacgctgata tcaaaacggt catccagcgc gccaatgaaa
    17821 acaagcacac cagcacaagc gagatagaga gatgcatgcg gaatatagta atcaacaatt
    17881 ccgaacgtga agcaaatccc tgcgtaaacc gaaatccccc caacgagagg tatcaatccc
    17941 tggtgacgtt tgcggaagtt tggtttatcc actaaaccga ctttttttgc caccttacgg
    18001 gcaaaaaaca gaaacagtgt cgtgaataaa aaaatactga tgagatcagt actcactgtc
    18061 agtaaattca caatgcatgc tctcagagaa aattattagc agaagtataa ccacgaagac
    18121 ctttattcag aagggaaagt ccgttccgaa cccaccagcc ctgtgcaaaa attaaccatt
    18181 tgatatgcaa attgcataac acaccagcaa attccaggaa ttttcctagc atcgccagcg
    18241 cggcattaag attacagata taaaagcaaa acgccacgta aacacgtggc gtttttggca
    18301 taagacaaat ttatgagcgt ttcatcattt cgaagaaatc gtcattggtc ttggtcattg
    18361 ccagtttatt aatgaggaat tccattgcat cgatttcgcc catcgggtga atgattttgc
    18421 gcaggatcca cattttctgc agttcttcct gagtcgtgag cagctcttct ttacgggtac
    18481 cggaacggtt gtagtcgata gccgggaaga cgcgtttttc agcgatctta cgagagaggt
    18541 gcagttccat gttgcctgta cctttaaact cttcgtagat aacttcgtcc attttagaac
    18601 cggtatcgat aagcgccgtc gcgataatgg tcaggctgcc gccctcttcc acgttacgcg
    18661 ccgcaccgaa gaagcgtttc ggacgatgca gggcgttggc gtccacacca ccggtcaaca
    18721 ctttacctga cgccggaaca acggtgttgt aagcgcgcgc cagacgagtg atggagtcga
    18781 gcagaatgat aacgtctttc ttgtgctcaa ccaggcgttt cgccttctcg atcaccattt
    18841 ccgcaacctg aacgtggcga gatgcgggtt cgtcaaaggt agaagcaaca acttcacctt
    18901 ttaccagacg ctgcatctcg gttacttctt ccggacgttc gtcgatcagc agaaccatca
    18961 gcacacaatc cgggtggttg taagcaatgc tctgagcaat gttctgcagc agcatggttt
    19021 taccggcttt cggcggtgcc acaatcagac cacgctgacc acgaccgata ggtgatgcca
    19081 gatccagtac gcgagcggtt aaatcttccg tagaaccgtt accacgttcc atacgcagac
    19141 gagagtttgc gtgcagcggg gttaagttct caaagaggat tttgttgcgg gcgttttcag
    19201 gtttgtcgaa gttaacttcg ttaactttca gcagcgcaaa atagcgttca ccttctttcg
    19261 gcgggcgaat cttaccagag atggtatcac cagtgcggag gttgaaacgg cggatttggc
    19321 taggggaaac gtagatgtca tcaggaccgg cgaggtagga gctgtctgcg gaacggagga
    19381 aaccaaatcc atcctgcaat atctccagta cgccatcacc aaagatatct tcgccactct
    19441 ttgcgtgctg cttcaggatg gcaaaaataa tgtcctgctt acgcatacga gccaggtttt
    19501 ccagccccat attttcgccg agagtgatca gctcagaaac cggcgtattc tttaattcgg
    19561 taagattcat agtggtgtga gttcttaaac ttggggtaaa tctcgaactt aatgttgtga
    19621 atggtatggc aggatcatcc atgcctgttt acggtcatcg actcatgtct tttcgctgcc
    19681 tggtcacagg agagtacgca gaactgaaac gacaagacgg attgagtgac gagccagaaa
    19741 tctgttcact tcgcatttaa gataaaacgg gaagcgttgg aaattacaag attcaaactt
    19801 aataaggtat gtttaatacg aagtcaacac taagttagca tgactcacgc cgggcgtcca
    19861 gtttttagcg acggggcacc cgaacatgaa attcccttac gccaggttag cgtcgaggaa
    19921 ctctttcaac tgacctttag acagtgcgcc cactttggtt gccgccactt caccgttttt
    19981 gaacagcagc agagtcggga taccacggat gccatatttc ggcgcagtgc cagggttttg
    20041 atcgatgttc agttttgcaa cggtcagttt gccctgatat tcgtcagcga tttcatccag
    20101 aatcggggcg atcattttgc acggaccgca ccactctgcc cagaaatcga cgaggatcgc
    20161 cccgtccgct ttgagtacat ccgtgtcaaa actgtcgtca gtcaggtgaa taattttatc
    20221 gctcatatat aactccacag gaataagcct ggcgtgttgg tttcgttgtt ggtgtaacat
    20281 taaccaacta aaggttgact ttatttcacc ggatacgctt tcgtaaagca atagtaagct
    20341 gatattctac cacactatga gcaaaacaca tttaacagaa cagaagtttt ccgacttcgc
    20401 cctgcatccg aaggttgtag aagcccttga aaaaaaaggg tttcataact gtacgcccat
    20461 tcaggcactg gcccttccgc tgacgctggc gggtcgtgac gtagccgggc aggcgcaaac
    20521 cggtaccggg aaaacgatgg cgtttcttac gtcaacgttt cattatcttc tctctcatcc
    20581 cgcgattgcc gatcgcaagg tgaatcagcc gcgtgcctta attatggcac cgacgcgtga
    20641 acttgccgtg cagatccacg ccgacgcgga accgctggcg gaagctaccg gcctgaagct
    20701 gggtctggct tacggtggtg atggctacga caaacagctg aaagtgctgg aaagcggcgt
    20761 tgacattctg attggcacca cgggtcgttt aattgactac gccaagcaga accacattaa
    20821 cctcggtgcc attcaggtgg tggtactgga cgaagccgat cgcatgtacg atctgggctt
    20881 tattaaagat atccgctggc tgttccgccg tatgccgcct gcaaaccagc gcctcaacat
    20941 gctgttctcc gccacgcttt cgtaccgggt acgtgaactc gcgttcgagc agatgaacaa
    21001 tgccgaatat attgaagtgg aaccggaaca gaaaacgggc caccgtatta aagaagagct
    21061 tttctaccct tctaacgaag aaaaaatgcg tttgctgcaa acgctgatcg aagaagagtg
    21121 gccagacaga gcgattattt tcgccaacac caaacaccgt tgtgaagaga tctggggcca
    21181 cctggcggca gatggtcatc gtgtcggttt attgacaggc gatgtcgcgc agaaaaaacg
    21241 tctgcgtatt cttgatgaat ttacccgtgg ggatctggat attctggttg ccaccgacgt
    21301 tgccgcgcgt ggtttgcata ttcctgctgt gacacacgtc tttaactacg atttacccga
    21361 tgactgtgaa gattacgttc accgtattgg tcgaacaggt cgcgcaggcg caagcggtca
    21421 ctctattagc ctggcgtgtg aagagtatgc attgaatttg cctgctattg aaacctatat
    21481 tggtcactca attccggtaa gtaaatacaa tccggacgca ttgatgaccg atctgccaaa
    21541 accgctgcgc ctcacgcgcc cgcgcacagg caatggtccg cgtcgtactg gcgctccgcg
    21601 taatcgtcgt cgttcaggtt aaagaaaaaa tgtcatactc caaatcattc aagctgtatc
    21661 aagatggcaa gagaatgaat cccgatgcgc ttactgtcgt aagtgattca ggtgaaagaa
    21721 tgcagccaac acagagacag attgaaggat gaagagtatg ggttccacct cgtcgctgta
    21781 tgcagccatt gatctcggtt cgaatagttt tcatatgctg gttgtgcgcg aggtggctgg
    21841 aagcatccag acgctgacgc gaataaaacg caaagtgcgt ctggctgctg gcctgaatag
    21901 cgaaaatgcc ctgtctaatg aagcaatgga gcgcggttgg caatgtctgc gcctgtttgc
    21961 tgaacgtctg caagatatcc ctccctcgca aattcgcgtt gtcgctacgg cgacattacg
    22021 ccttgccgtc aatgcggggg attttattgc caaagcgcag gaaatcctcg gttgtccggt
    22081 acaggtgatc agcggtgaag aggaagcgcg tctgatttat cagggcgttg ctcacaccac
    22141 tggcggtgcc gatcagcgcc tggtggtgga tatcggcgga gccagtactg aactggtaac
    22201 cggcacgggt gcacaaacta cctctttgtt cagcctgtcg atgggctgcg ttacctggct
    22261 ggaacgctat tttgccgatc gtaatctggg gcaggaaaat tttgatgctg cagaaaaagc
    22321 ggcacgcgaa gtgttacgtc cggttgccga tgaattacgg tatcacggct ggaaagtgtg
    22381 cgttggcgct tccggcaccg tgcaggcgtt acaggaaatc atgatggcac aggggatgga
    22441 tgaacgcatt accctggaaa agttgcagca actgaaacaa cgcgccattc attgcggtcg
    22501 gctggaagag ctagagattg acgggctgac gctggaacgt gcgttagtgt tcccgagtgg
    22561 tctggcaatc ctgatagcca tttttaccga actgaatatt cagtgtatga ccctggcggg
    22621 cggtgcgctg cgtgaaggcc tggtctacgg tatgttgcat cttaccgtcg agcaggatat
    22681 tcgcagccgt acgctgcgta atattcagcg ccgctttatg atcgatattg atcaggcaca
    22741 gcgcgtagcc aaagttgcgg ctaacttctt cgatcaggtg gaaaatgaat ggcatcttga
    22801 agcaataagc cgcgatttgc tcatcagcgc ctgtcagctt catgaaatcg gcctgagcgt
    22861 tgacttcaaa caagcgccgc aacacgctgc ttatctggta cgtaatctgg atcttcccgg
    22921 ttttaccccc gcacagaaaa aactgctggc gacgctactg ctcaaccaga ctaatccggt
    22981 cgatctctca tcgctgcatc agcaaaatgc cgtaccaccg cgcgtcgcag aacaactctg
    23041 ccgtttactc cgcctggcta tcatttttgc cagccgtcgc cgtgacgatc tcgtgccaga
    23101 gatgacatta caggctaacc atgaactgtt gaccttgacg cttccgcaag gttggctaac
    23161 ccaacatccg ctgggtaaag agattattga tcaggaaagc cagtggcaga gctatgtcca
    23221 ctggccgctg gaagtgcatt aatgagtaag tgccggatgc gatgctgaca catcttttcc
    23281 ggccttgatt atttccctcg ttttgccgcc atcatcgctt tcagattcgc cagatggctt
    23341 tgccctttct gcatccgttc ttcggcgctg accactttgc gctcctgttc ccaaatcaaa
    23401 tcatcctgcg gcagctccag caggaagcgg ctcggctccg ggcgcaccag ttcgccgtac
    23461 tgacggcgtt ctttacacag cgtaaaggtc aattccttct gggcgcgggt aatgccgaca
    23521 taggccagcc gccgctcctc atcgatatta tcttcatcga tactgctctg gtggggcaaa
    23581 aatccttctt ccataccaac catgtagaca tagggaaact ccagtccttt tgacgcgtgc
    23641 aaagtcatca gttgcacctg atccagctct tcctcactct cgccgcgctc catcatgtcg
    23701 cgcaaagtaa agcgcgtcac cacctgggtg agcgtcatcg gctcatccag ttcgctgcct
    23761 tcgagcatct ccgtcatcca gctaaacagt tggttgacgt tcttcatgcg catttcggca
    23821 gctttcggac tgggcgatgt ttcgtacagc caggattcat aatccatgcc gtggatcaga
    23881 tcgcgcaccg cggcaatcgg ctcccgctcc gccagacgct ggatttctgc cagccagtga
    23941 gtgaagcggg tcaatgcttc ataaccacgt ccgctaagcg tctgactcag gcccatatca
    24001 aagctggcgg taaacatgct tttattgcgc gtcatcgccc attcacctag ctttttcagc
    24061 gtcgccgggc caatctctcg cttcggcgtg ttaacgatac gcagaaatgc gctgtcatcg
    24121 tccgggttag tcagcacgcg cagataagcc agcaagtcct tgatttcagg acgagagaaa
    24181 aacgacgtac cgccagatat tttgtacggg atgcggtttt gcatcaggaa tttttcaaat
    24241 actcgcgact gatggttgcc gcgataaaga atggcgtaat ctttgtactg cgttttattg
    24301 acgaagtgat gggcgatcag ctcgccggtg acgcgctcgg cttcatgttc ctcgttattc
    24361 gcgcttaaaa cttttagctc tgtgccgtaa cccagttcgg agaacagacg cttttcaaag
    24421 acgtgcgggt tattggcgat caggatgttc gccgctttca aaatacgtcc ggaagagcgg
    24481 tagttctgct caagcttaat caccttcagc gccggaaaat cctggctcag cagcaccaga
    24541 ttctgcggac gtgcgccgcg ccaggagtag atcgactggt cgtcatcgcc caccacggta
    24601 aagcgcgcgc ggctgcccac cagcagtttc accagttcat actggctggt gttggtgtcc
    24661 tgatactcat ccaccagcag gtagcgaatt ttgttctgcc agcgctcgcg gacttcttca
    24721 ttgcgttgca gcagcaacgt tggcaataaa atcagatcat cgaaatcgag aacgttacag
    24781 gctttcaggt gtgcatcata cagcccataa caatgggcaa aaatacggtc ccgctcgccc
    24841 tttgcctctg ccgccgcctg cgccggtgtt ttgagatcgt tcttccagtt ggagatggtc
    24901 gaaattagct gttgcaggag aactttgtca tcttcaatca gcccctcggt caactcttta
    24961 agcaaagcaa gctgatcggt atcgtcaaac agcgaaaagt tagccttcat ccccagcgcc
    25021 gcatactcgc gtttgatgat atccagcccc agcgtgtgga aggtggagat catcagccca
    25081 cgcgcctctt tgcgccccag cgtctgcccg acacgctctt tcatctcgcg tgccgcctta
    25141 ttggtaaagg tcaccgccgc gatatgtcgc gcctgataac cgcaaccgcg gatcagatgg
    25201 gcgattttat tggtgataac acgagtttta ccggaacccg cgcccgccag caccaggcag
    25261 gggccggtaa cgaattcgac agcttgttgt tggccggggt ttagacgcat aggaaaatcg
    25321 ctcaatgaaa gtcgggaggg gaaaataaca gcgtggtagt atagccagcc tcatccttac
    25381 cactcaaggc acgatcatgg caaaaacagc agcagcactg catatccttg taaaagaaga
    25441 gaaactggct ctggatcttc tcgagcagat caagaatggt gccgatttcg gcaagctggc
    25501 gaagaaacac tccatttgcc cgtcaggcaa acgcggcggt gatttgggtg aattccgcca
    25561 gggtcaaatg gttccggcgt tcgataaagt ggtgttttct tgcccggtgc tggaaccaac
    25621 cggcccgctg catacccagt tcggttacca catcattaag gtgctgtacc gtaactaaca
    25681 gcaaggcctt ctccgggaga aggccttgaa ctctgaatcg ctattaaccc gcaacagcaa
    25741 tacgtttcat atccgtcata tagccgcgca gtttcttacc tacctgctca atcgcatggc
    25801 tgcgaatcgc ttcatttaca tcacgcagct gcgcgttatc taccgcacct tccggaatag
    25861 ctttacccag gtcgcccggt tgcagctctg ccataaacgg tttcagcagc ggcacacaag
    25921 cgtaagagaa cagatagtta ccgtactcgg cagtatcgga gataaccacg ttcatttcgt
    25981 acagacgctt acgggcgatg gtgttggcaa tcagcggcag ttcgtgcagt gattcatagt
    26041 aagcagattc ttcgatgatg ccggaatcaa ccatggtttc aaacgccagt tcaacgcctg
    26101 cttttaccat cgcgatcatc agtacgcctt tatcgaagta ctcctgttcg ccgattttgc
    26161 cttcatactg cggcgcggtt tcgaacgcgg ttttgccggt ctcttcacgc caggtcagca
    26221 atttcttatc gtcgttggcc cagtcagcca tcatgccgga ggagaattcg ccggagatga
    26281 tgtcgtccat atgtttctgg aacagcggcg ccatgatctc tttcagttgc tcagagagcg
    26341 cgtaagcacg cagtttcgcc gggttagaaa gacggtccat catcagggtg atgccgccct
    26401 gtttcagcgc ttcggtgatg gtttcccaac cgaactgaat cagtttttct gcgtatgccg
    26461 gatcggtgcc ttcttccacc agcttgtcga agcacagcag agaaccagct tgcaacatac
    26521 cgcacaggat ggtttgctcg cccatcaggt cagatttcac ttccgcaacg aaagaggatt
    26581 ccagaacgcc cgcacggtga ccgccggttg cagccgccca tgctttagcg atcgccatgc
    26641 cttcgccttt cggatcgttt tccgggtgaa cggcaatcag cgtcggtacg ccgaatccac
    26701 gtttgtactc ttcacgtact tcggtgccag ggcatttcgg cgcaaccatt acgacggtga
    26761 tgtctttacg gatctgctca cccacttcta cgatattgaa accatgagag tagcccagcg
    26821 ccgcgccgtc tttcatcagc ggctgaacgg aacgcactac gtcagagtgc tgcttgtctg
    26881 gcgtcaggtt aaccaccaga tccgcctgcg ggatcagttc ttcgtaagtg ccaactttga
    26941 agccgttttc ggtcgcttta cgccaggatg cgcgcttctc ggcaatcgct tctttacgca
    27001 gagcgtagga gatatcgaga ccagaatcac gcatgttcag gccctggttc agaccctgtg
    27061 caccacagcc gacgatgact acttttttac cctgaaggta gctcgcgcca tcggcgaatt
    27121 catcgcgccc cataaagcga catttgccca gctgtgccag ctgctggcgc agattcagtg
    27181 tattgaagta gttagccatg gtgattcctc gtgatgttgt gcttcttatt gttcggttcg
    27241 cgtttgcgag attgaattca ctatatgaca ggaaatttat tgcggaaatt gatatattca
    27301 caacgtcaca ttgcaatttt tgcaacgtca acattgaggg ctgcgccatt ggatttacgc
    27361 gatctgaaaa ccttcctgca tctggcggaa agccgccatt ttggccgcag cgcgcgggca
    27421 atgcacgtta gcccgtccac gctctctcgg cagattcagc gcctggaaga cgatctcggt
    27481 cagccgctgt ttgtgcgcga taaccgcacg gtaacgctaa ccgaagcagg cgaagaactg
    27541 cgcgttttcg cccagcaaac gctgttgcag tatcaacagc tgcgccacac catcgatcag
    27601 caaggaccgt cgctctctgg cgaattacat atcttctgct cggtgaccgc tgcctacagc
    27661 catctgccgc cgattctcga tcgcttccgc gcggaacacc cgtcggtgga gattaaactc
    27721 acgactggtg atgcggcaga tgcgatggaa aaggtcgtca ctggcgaagc ggatctggcg
    27781 attgccggga aaccggagac tctgcccggc gcagtggcat tttcgatgct ggagaatctg
    27841 gcagtagtgc tgattgcccc tgcgctaccc tgcccggtgc gtaaccaagt ttcggcagat
    27901 aagccggact ggtctacagt gccgtttatc atggccgatc agggaccggt gcgccggcgc
    27961 attgaactgt ggtttcgacg caacaaaatc agcaacccga tgatttacgc tacggtcggc
    28021 gggcatgaag cgatggtgtc gatggtagcg ctcggctgtg gcgtagcctt gttgccggaa
    28081 gtggtgctgg aaaacagccc ggaaccggtg cgtaaccgcg tgatgatttt agagcgcagt
    28141 gatgaaaaaa cgccgtttga actgggcgtg tgtgtgcaga aaaagcggct acatgagccg
    28201 ctgattgagg cattctggaa gattttgccg aaccattaac ccgccaaaaa gaacctgaac
    28261 gccgggttat tggtttcgtc gtggcaatcg taacccaact cattcaaccg ggtttcgaaa
    28321 tccggttcat ggtcgccaag ttcgaacgcc gccagtacgc gcccgtagtc ggtgccatgg
    28381 ctgcgatagt ggaacaaaga aatgttccag tgcgtaccca gcgtgttgag gaagcgcaac
    28441 agcgcgcccg gcgattccgg gaattcgaag ctgtagaggc gttcctgcaa cggatgcgac
    28501 ggacgcccgc cgaccatata gcgcacatgc agcttcgcca tttcgtcgtc ggagagatcc
    28561 actacgctgt agccgccatc gttgagcatc tgcaaaattt ctttgcgctc ttcgagacca
    28621 cggctcaggc gcacaccaac aaaaatgcag gcgtttttgg catcggcaaa acggtagttg
    28681 aactcggtga ccgagcgccc gccaagcagc tggcagaact tcaggaagct gcccttctct
    28741 tccgggatgg ttaccgccag caatgcttca cgctgttcgc ccagttcgca acgttccgag
    28801 acgtagcgca ggccgtgaaa gttcacgtta gcaccagaaa gaatatgcgc cagccgctca
    28861 ccgcgaatgt tgtgctgggc gatatatttt ttcattcccg ccagtgccag cgcgccagag
    28921 ggttccgcca ccgcgcgcac atcttcgaac aggtctttca tcgccgcaca tatggcatcg
    28981 ctatcgacgg tgatgatgtc gtcgagatac tcctggcata aacggaaggt ttcgtcgccg
    29041 atgcgtttca ccgcaacacc ttcggcaaac agccccacgc gtggcagatc aaccggatga
    29101 cctgcattca gcgccgcttt caggcaggcg gagtcttccg cttccacggc aatcactttg
    29161 atttgcggca tcagttgttt gatcagtacc gccacgcccg ccgccagacc gccgccgccg
    29221 actggcacaa atacgcggtc gagatgggcg tcctgctgga gcagttccag cgccagcgtg
    29281 ccttgcccgg cgatcaccat cggatggtcg aacggcggca cccaggtgaa gccctgctgc
    29341 tgtgatagtt cgatcgcttt cgcttttgct tcatcaaagt tcgcgccgtg aagaagcact
    29401 tcgccgccga agccgcgcac cgcgtcgact ttgatatcgg cggtggcggt tggcatgacg
    29461 atcagtgcct tcacgcctaa ccgtgcggaa gaaaacgcga cgccctgcgc gtggttaccc
    29521 gcagaagcgg tgatcacgcc gtgcgctttc tgttcttccg tcaggcccgc catcatggcg
    29581 tatgcgccgc gcagcttaaa gctgtgcact ggctgacggt cttcgcgttt caccagaatc
    29641 acgttatcga gacgcgacga gagtttttcc attttttgta gcggcgtgac ctgcgccgct
    29701 tcgtagaccg gcgcgcgcag caccgctctt aaatattcgg cgccttccgg ggcgccggag
    29761 agaggttgtg agtctgccat cattaacccc ccagtttcga tttatcacgc accgcgcctt
    29821 tgtcggcgct ggttgccagg ctggcgtagg cacgcagcgc aaaggaaacc tgacgttcac
    29881 gatttttcgg tgtccaggct ttgttgccac gagcttcctg cgcttcacga cgcgccgcca
    29941 gttcggcatc gcttacctgt aactgaatgc cacggttcgg aatgtcgata gcgatcaggt
    30001 caccatcttc aatcaggcca atgctgccgc cacttgccgc ttccggtgag acgtggccga
    30061 tggaaagacc agaggtgccg ccagagaaac gaccgtcggt gatcagcgca caggctttac
    30121 cgagacccat tgatttcagg aaactggttg ggtagagcat ttcctgcatc cccggaccgc
    30181 ctttcgggcc ttcatagcga attactacca catctccggc gacaacttta ccgccgagaa
    30241 tcgcttctac cgcgtcgtcc tggctttcgt agactttcgc cgggccagtg aatttgagga
    30301 tgctgtcatc gacgcctgcg gttttaacga tgcagccgtt ttctgcgaaa ttaccgtaga
    30361 gcaccgccag gccgccgtct ttgctgtagg cgtgttccag cgagcggata cagccattgg
    30421 agcgatcgtc gtccagcgta tcccagcggc aatcctgcga gaatgcctgt gtagtacgaa
    30481 tgcccgccgg gccagcgcgg aacatatttt ttaccgcatc atcctgggtc acgatgatgt
    30541 cgtattgttc cagcgtttgc ggcaacgtca ggccaagtac atttttcaca tcacggttca
    30601 gtaaccccgc acgatccagc tcgccgagaa taccgataac accaccagca cggtgaacat
    30661 cttccatatg gtatttttgg gtgctcggcg caactttaca cagctgcggt actttgcggg
    30721 agagcttatc gatatcactc atggtgaagt cgatttccgc ttcctgcgcc gccgccagca
    30781 ggtggagaac ggtgttggta gagccgccca tcgcgatatc cagcgtcatg gcgttttcaa
    30841 acgccgcctt actggcgatg ttacgcggca gtgcgctttc atcatcctgt tcgtagtaac
    30901 gtttggtcaa ttcaacaatg cgtttaccag cattgaggaa cagttgcttg cgatcagcgt
    30961 gggttgccag cagcgagccg ttgcccggct gcgacaggcc cagcgcttcg gtcaggcagt
    31021 tcattgagtt agcagtaaac atcccggagc aggaaccgca ggttgggcag gcggatcgtt
    31081 caacctgatc gctctgggag tcagaaactt tcgggtctgc gccctggatc atcgcatcaa
    31141 ccagatcgag cttaatgatc tgatcggaca gtttggtttt cccggcctcc atcgggccgc
    31201 cggaaacaaa gatcaccgga atattcaggc gcagggaagc catcagcatc cccggggtga
    31261 ttttgtcgca gttagagatg cagaccatgg cgtcggcgca gtgggcgttg accatatatt
    31321 caacggaatc agcgatcagt tcgcgagatg gcagtgaata aagcatgccc ccgtggccca
    31381 tagcgatccc atcatccacc gcaatggtgt tgaactcttt ggcaacgccg ccagccgctt
    31441 caatttgttc ggcaaccagt ttaccgagat cgcgtagatg gacgtgaccc ggtacaaatt
    31501 gggtgaacga gttcacaacc gcgataatcg gcttaccgaa atcggcgtcg gtcattccgg
    31561 tggcgcgcca cagcgcacga gcacccgcca tattacgacc atgagtggtg gtggcggaac
    31621 ggtacttagg catactttat ttactcccag tgtctgtctc gcaaatggga cggtgcgtgc
    31681 cgtcccattt tttgtattta ttgattaact tgatctaacc agccccattt atcttcggtc
    31741 tcgccagtga agaggccgaa gaaggcttgc tgaatgcgtt tggtgaccgg gccgcaacgg
    31801 ccttcgccaa cctgaatacc atctacgctg cgcactggcg tgatttctgc cgccgtaccg
    31861 gacataaaca cttcatccgc caggtacagt gattcgcgcg acagcacctg ctcacgcact
    31921 tcaattccca gctctttcgc cagtttgatg atggcgtcac gggtaatacc cggcagcgcg
    31981 gaggaggtga acggtggggt gaacagcacg ccgtctttca cttcaaacag gttttcgcct
    32041 gcgccttcag agatataacc gttcacatcc agcgcgatac cttcctgata accgtggcgg
    32101 cgcgcttcgc tacccaccag cagggaagag aggtagttac caccggcttt tgccgccgtc
    32161 gggatggtgt ttggtgctgc gcggttccag gaggaaacca tcgcatcgat cccctgctcc
    32221 agcgcttctg cgcccagata cgctccccac gggaaagcgg cgataatcac gtcggttgag
    32281 tatcccgctg gcgggtttac gcccatgcca acatcaccaa cgaagatcag cggacggata
    32341 taggcgctgg tgagattgtt tttgcggatc acgtcacgac aagcttccat cagctcatca
    32401 atgctctgcg aaaccgggaa gcgatagatt ttggcggagt catgcagacg ctgcatatgc
    32461 tcacgatggc ggaatacaac cggtcctttg tgcgagtcgt agcaacggat gccttcaaaa
    32521 accgaggtgc catagtgcag cgcgtgcgac atcacatgca ccttcgcgtc ttcccagcga
    32581 accatctccc cattgaacca aatgtaatca gctttcttcg tggtcatttt tatattcctt
    32641 ttgcgctcag gcgcggattt gttgtgatgt ggttgtgctc tggcagatag caacgtgtgc
    32701 gacgtccacc agtttattta actgactaaa cagtaagtcg accgaccgtg ggctggcaac
    32761 ggtcaattcg atatttatat tttgtgcatc gctggcggca gccatattca ttgagcagac
    32821 gtggaaacca cgatgacgca ccacgcgtaa aacacgttct aaggtttccg gattgaagcg
    32881 agccgataca ttgacctgat gttgcatcat gataatttct ccaacatttc agaattactg
    32941 gcaccaggcg gcaccagcgg ccagacgttc tcaagttcgt cgattgagac atgaagcagg
    33001 tatggcccat cactgttcag catggtgtcg agtgccgctt caacctggtc tttacgggtg
    33061 atgtgttggc cagggatgcc gaaggcgctg gctaacatga ggaaatcggg gttatcggta
    33121 agggttgttt cgctgtatcg ttcctggaaa aacagttgct gccattgtcg aaccatccct
    33181 aaccgttggt tatcgagtaa gacgattttc aacggtaact gcttgcgttt tacggtgccc
    33241 agctcttgca cattcatcat gaaagagccg tcaccggaga tacagacgac ggtatcgttc
    33301 ggtcgcgcga cttgtgcgcc aaccgccgcc ggtaaaccaa aacccatggt gcctaagccg
    33361 ctggaggtga tgaaattttc cgggcgagtg tgggcgatgt gctgcgcagc ccacatctgg
    33421 tgctgcccca catctgtggt cacgacgcaa tccgcaggtt ttcgatccga cagttgtttt
    33481 aacaacaacg gcgcgtagat agcgtcaccg ggatggtcgt aacgccaggt atgttcatca
    33541 cgcagctgcg cgcagtattg ctgccagtca ttgatattta acggctgctg taatgctggt
    33601 aacagagcat ttaaatcacc ctgtaatgcc acatgtgcct gacgcagctt gttcatttct
    33661 gccgggtcga tatccatatg gataacgctg gcgtgtggtg cgaaggtgtt cagtttgcca
    33721 gtcacccggt catcaaaacg tgcacccacg gcgatcagta gatcgcactc ctgcactgcg
    33781 aagttcgccg ctttggtgcc atgcatcccc agcatgccca gatagtacgg ataatctgct
    33841 tctactgcgc ccagcccttt caacgtacag gtggcaggca tttttgtggc agcgagaaat
    33901 tcacgcaaag ccgaaactgc ctgcgccatg cccacgccac cgccaacgta cagcatcggt
    33961 ttttgcgctt ttgccagcat ctgacgcgct tgctcgactt cggcatgtga gaaagtcact
    34021 tcgttttcaa cagtggtgaa ccacggttcc agatcgccgc tggctaactg gatatctttt
    34081 ggaatatcga ccaaaaccgg accaggacga ccagagctgg cgacgtcaaa cgcttcggcc
    34141 atgatacgcg gtaactcttc cagtgattgc accaggaaac tgtgcttggt gcaggctaac
    34201 gacaacccca gaatatccac ctcctgaaat gcgtcagtgc cgataaacgg tgcggatacc
    34261 tgaccggtga tagcaacaac ggggatggaa tctaacagcg cgtccgcaag cccggttatc
    34321 aggttggttg cacctgggcc agacgtggcg atgcaaacgc cagttttgcc ggtagcacgg
    34381 gcataaccga tagccgccat tgctgcacct tgctcgtgtc ggcacagcaa gtgttccacg
    34441 ccaccgtcat acaatgcatc gtaaaccggc ataattgcgc cacccggata accgaaaacg
    34501 gtgttcacac cctgtgcccg caacgcatgt accacccact gtgcgccatt catagttagt
    34561 tccccgtcct gaatcttgag aaacagaatt ttgtgctgtt attcattgtc tgctcctcag
    34621 ttatgttttt aaggtcaaaa aaaacccccg gacctttcgg tgcgggggtc ttagttcgtt
    34681 aaggcttgat ctctaagcct ttcctcgtcc aagtgcagcc ccgcacggtg ggataataat
    34741 caccaccacg ctaatcacga ccaggctaat cactcgtaga agggctgtca ttttgtcttt
    34801 tcttgcatct tgttcgaagg aatgcctaaa gagttaccat agattttgcg aa
//
LOCUS       DADSYM010000033        34545 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2415
            SAMN08039579-rid11723173.denovo.033, whole genome shotgun sequence.
ACCESSION   DADSYM010000033 DADSYM010000000
VERSION     DADSYM010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039579
            Sequence Read Archive: SRR6362533
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 34545)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34545)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980162.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 03:39:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,484
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 4,347
            CDSs (with protein)               :: 4,347
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 43 of 85
            Pseudo Genes (incomplete)         :: 37 of 85
            Pseudo Genes (internal stop)      :: 19 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34545
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039579-rid11723173.denovo.033"
                     /strain="LGE2415"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8302 N 95.9628 W"
                     /collection_date="2015-08-24"
     gene            complement(45..770)
                     /gene="phoU"
                     /locus_tag="J1Y83_001345"
     CDS             complement(45..770)
                     /gene="phoU"
                     /locus_tag="J1Y83_001345"
                     /EC_number="3.5.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HBA7629506.1"
                     /translation="MDSLNLNKHISGQFNAELESIRTQVMTMGGMVEQQLSDAITAMH
                     NQDSDLAKRVIEGDKNVNMMEVAIDEACVRIIAKRQPTASDLRLVMVISKTIAELERI
                     GDVADKICRTALEKFSQQHQPLLVSLESLGRHTIQMLHDVLDAFARMDIDEAVRIYRE
                     DKKVDQEYEGIVRQLMTYMMEDSRTIPSVLTALFCARSIERIGDRCQNICEFIFYYVK
                     GQDFRHVGGDELDKLLAEKDSDK"
     gene            complement(785..1558)
                     /gene="pstB"
                     /locus_tag="J1Y83_001346"
     CDS             complement(785..1558)
                     /gene="pstB"
                     /locus_tag="J1Y83_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000063125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HBA7629507.1"
                     /translation="MSMVETAPSKIQVRNLNFYYGKFHALKNINLDIAKNQVTAFIGP
                     SGCGKSTLLRTFNKMFELYPEQRAEGEILLDGDNILTNSQDIALLRAKVGMVFQKPTP
                     FPMSIYDNIAFGVRLFEKLSRADMDERVQWALTKAALWNETKDKLHQSGYSLSGGQQQ
                     RLCIARGIAIRPEVLLLDEPCSALDPISTGRIEELITELKQDYTVVIVTHNMQQAARC
                     SDHTAFMYLGELIEFSNTDDLFTKPAKKQTEDYITGRYG"
     gene            complement(1649..2539)
                     /gene="pstA"
                     /locus_tag="J1Y83_001347"
     CDS             complement(1649..2539)
                     /gene="pstA"
                     /locus_tag="J1Y83_001347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HBA7629508.1"
                     /translation="MAMVEMQTTAALAESRRKMQARRRLKNRIALTLSMATMAFGLFW
                     LIWILMSTITRGIDGMSLALFTEMTPPPNTEGGGLANALAGSGLLILWATVFGTPLGI
                     MAGIYLAEYGRKSWLAEVIRFINDILLSAPSIVVGLFVYTIVVAQMEHFSGWAGVIAL
                     ALLQVPIVIRTTENMLKLVPDSLREAAYALGTPKWKMIASITLKASVSGIMTGILLAI
                     ARIAGETAPLLFTALSNQFWSTDMMQPIANLPVTIFKFAMSPFAEWQQLAWAGVLIIT
                     LCVLLLNILARVVFAKNKHG"
     gene            complement(2539..3498)
                     /gene="pstC"
                     /locus_tag="J1Y83_001348"
     CDS             complement(2539..3498)
                     /gene="pstC"
                     /locus_tag="J1Y83_001348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008806729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstC"
                     /protein_id="HBA7629509.1"
                     /translation="MAATKPAFNPPGKKGDIIFSVLVKLAALIVLLMLGGIIVSLIIS
                     SWPSIQKFGLAFLWTKEWDAPNDIYGALVPIYGTLVTSFIALLIAVPVSFGIALFLTE
                     LAPGWLKRPLGIAIELLAAIPSIVYGMWGLFIFAPLFAVYFQEPVGNIMSNIPIVGAL
                     FSGPAFGIGILAAGVILAIMIIPYIAAVMRDVFEQTPVMMKESAYGIGCTTWEVIWRI
                     VLPFTKNGVIGGIMLGLGRALGETMAVTFIIGNTYQLDSASLYMPGNSITSALANEFA
                     EAESGLHVAALMELGLILFVITFIVLAASKFMIMRLAKNEGAR"
     gene            complement(3585..4625)
                     /gene="pstS"
                     /locus_tag="J1Y83_001349"
     CDS             complement(3585..4625)
                     /gene="pstS"
                     /locus_tag="J1Y83_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HBA7629510.1"
                     /translation="MKVMRTTVATVVAATLSMSAFSVFAEASLTGAGATFPAPVYAKW
                     ADTYQKETGNKVNYQGIGSSGGVKQIIANTVDFGASDAPLSDEKLAQEGLFQFPTVIG
                     GVVLAVNIPGLKSGELVLDGKTLGDIYLGKIKKWDDEAIAKLNPGLKLPSQNIAVVRR
                     ADGSGTSFVFTSYLAKVNEEWKNNVGTGSTVKWPIGLGGKGNDGIAAFVQRLPGAIGY
                     VEYAYAKQNNLAYTKLISADGKPVSPTEENFANAAKGADWSKTFAQDLTNQKGEDAWP
                     ITSTTFILIHKDQKKPEQGTEVLKFFDWAYKTGAKQANDLDYASLPDSVVEQVRAAWK
                     TNIKDSSGKPLY"
     gene            complement(4938..6767)
                     /gene="glmS"
                     /locus_tag="J1Y83_001350"
     CDS             complement(4938..6767)
                     /gene="glmS"
                     /locus_tag="J1Y83_001350"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HBA7629511.1"
                     /translation="MCGIVGAIAQRDVAEILLEGLRRLEYRGYDSAGLAVVDAEGHMT
                     RLRRLGKVQMLAQAAEEHPLHGGTGIAHTRWATHGEPSEANAHPHVSEHIVVVHNGII
                     ENHEPLREELKARGYTFVSETDTEVIAHLVNWELKQGGTLREAVLRAIPQLRGAYGTV
                     IMDSRHPDTLLAARSGSPLVIGLGMGENFIASDQLALLPVTRRFIFLEEGDIAEITRR
                     SVNIFDKTGAEVKRQDIESNLQYDAGDKGIYRHYMQKEIYEQPNAIKNTLTGRISHGQ
                     VDLSELGPNADELLSKVEHIQILACGTSYNSGMVSRYWFESLAGIPCDVEIASEFRYR
                     KSAVRRNSLMITLSQSGETADTLAGLRLSKELGYLGSLAICNVPGSSLVRESDLALMT
                     HAGTEIGVASTKAFTTQLTVLLMLVAKLSRLKGLDASIEHDIVHGLQALPSRIEQMLS
                     QDKRIEALAEDFSDKHHALFLGRGDQYPIALEGALKLKEISYIHAEAYAAGELKHGPL
                     ALIDADMPVIVVAPNNELLEKLKSNIEEVRARGGQLYVFADQDAGFVSSDNMHIIEMP
                     HVEEVIAPIFYTVPLQLLAYHVALIKGTDVDQPRNLAKSVTVE"
     gene            complement(6928..8298)
                     /gene="glmU"
                     /locus_tag="J1Y83_001351"
     CDS             complement(6928..8298)
                     /gene="glmU"
                     /locus_tag="J1Y83_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HBA7629512.1"
                     /translation="MLNNAMSVVILAAGKGTRMYSDLPKVLHTLAGKAMVQHVIDAAN
                     ELGAAHVHLVYGHGGDLLKQALKDDNLNWVLQAEQLGTGHAMQQAAPFFADDEDILML
                     YGDVPLISVETLQRLRDAKPQGGIGLLTVKLEDPTGYGRITRENGKVTGIVEHKDATD
                     EQRQIQEINTGILIANGADMKRWLAKLTNNNAQGEYYITDIIALAYQEGREIVAVHPQ
                     RLSEVEGVNNRLQLSRLERVYQSEQAEKLLLAGVMLRDPARFDLRGTLTHGRDVEIDT
                     NVIIEGNVTLGHRVKIGTGCVIKNSVIGDDCEISPYTVVEDANLAAACTIGPFARLRP
                     GAELLEGAHVGNFVEMKKARLGKGSKAGHLTYLGDAEIGDNVNIGAGTITCNYDGANK
                     FKTIIGDDVFVGSDTQLVAPVTVGKGATIAAGTTVTRNVGENALAISRVPQTQKEGWR
                     RPVKKK"
     gene            complement(8652..9071)
                     /gene="atpC"
                     /locus_tag="J1Y83_001352"
     CDS             complement(8652..9071)
                     /gene="atpC"
                     /locus_tag="J1Y83_001352"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001251972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HBA7629513.1"
                     /translation="MAMTYHLDVVSAEQQMFSGLVEKIQVTGSEGELGIYPGHAPLLT
                     AIKPGMIRIVKQHGHEEFIYLSGGILEVQPGNVTVLADTAIRGQDLDEARAMEAKRKA
                     EEHISSSHGDVDYAQASAELAKAIAQLRVIELTKKAM"
     gene            complement(9092..10474)
                     /gene="atpD"
                     /locus_tag="J1Y83_001353"
     CDS             complement(9092..10474)
                     /gene="atpD"
                     /locus_tag="J1Y83_001353"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017800291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HBA7629514.1"
                     /translation="MATGKIVQVIGAVVDVEFPQDAVPRVYDALEVQNGNERLVLEVQ
                     QQLGGGIVRTIAMGSSDGLRRGLDVKDLEHPIEVPVGKATLGRIMNVLGEPVDMKGEI
                     GEEERWAIHRAAPSYEELSNSQELLETGIKVIDLMCPFAKGGKVGLFGGAGVGKTVNM
                     MELIRNIAIEHSGYSVFAGVGERTREGNDFYHEMTDSNVIDKVSLVYGQMNEPPGNRL
                     RVALTGLTMAEKFRDEGRDVLLFVDNIYRYTLAGTEVSALLGRMPSAVGYQPTLAEEM
                     GVLQERITSTKTGSITSVQAVYVPADDLTDPSPATTFAHLDATVVLSRQIASLGIYPA
                     VDPLDSTSRQLDPLVVGQEHYDTARGVQSILQRYQELKDIIAILGMDELSEEDKLVVA
                     RARKIQRFLSQPFFVAEVFTGSPGKYVSLKDTIRGFKGIMEGEYDHLPEQAFYMVGSI
                     EEAVEKAKKL"
     gene            complement(10501..11364)
                     /gene="atpG"
                     /locus_tag="J1Y83_001354"
     CDS             complement(10501..11364)
                     /gene="atpG"
                     /locus_tag="J1Y83_001354"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HBA7629515.1"
                     /translation="MAGAKEIRSKIASVQNTQKITKAMEMVAASKMRKSQDRMAASRP
                     YAETMRKVIGHLAHGNLEYKHPYLEDRDVKRVGYLVVSTDRGLCGGLNINLFKKLLAE
                     MKTWTDKGVQCDLAMIGSKGVSFFNSVGGNVVAQVTGMGDNPSLSELIGPVKVMLQAY
                     DEGRLDKLYIVSNKFINTMSQVPTISQLLPLPASDDDDLKHKSWDYLYEPDPKALLDT
                     LLRRYVESQVYQGVVENLASEQAARMVAMKAATDNGGSLIKELQLVYNKARQASITQE
                     LTEIVSGAAAV"
     gene            complement(11415..12956)
                     /gene="atpA"
                     /locus_tag="J1Y83_001355"
     CDS             complement(11415..12956)
                     /gene="atpA"
                     /locus_tag="J1Y83_001355"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001176751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HBA7629516.1"
                     /translation="MQLNSTEISELIKQRIAQFNVVSEAHNEGTIVSVSDGVIRIHGL
                     ADCMQGEMISLPGNRYAIALNLERDSVGAVVMGPYADLAEGMKVKCTGRILEVPVGRG
                     LLGRVVNTLGAPIDGKGPLDHDGFSAVEAIAPGVIERQSVDQPVQTGYKAVDSMIPIG
                     RGQRELIIGDRQTGKTALAIDAIINQRDSGIKCIYVAIGQKASTISNVVRKLEEHGAL
                     ANTIVVVATASESAALQYLAPYAGCAMGEYFRDRGEDALIIYDDLSKQAVAYRQISLL
                     LRRPPGREAFPGDVFYLHSRLLERAARVNAEYVEAFTKGEVKGKTGSLTALPIIETQA
                     GDVSAFVPTNVISITDGQIFLETNLFNAGIRPAVNPGISVSRVGGAAQTKIMKKLSGG
                     IRTALAQYRELAAFSQFASDLDDATRKQLDHGQKVTELLKQKQYAPMSVAQQSLVLFA
                     AERGYLADVELSKIGSFEAALLAYVDRDHAPLMQEINQTGGYNDEIEGKLKGILDSFK
                     ATQSW"
     gene            complement(12969..13502)
                     /gene="atpH"
                     /locus_tag="J1Y83_001356"
     CDS             complement(12969..13502)
                     /gene="atpH"
                     /locus_tag="J1Y83_001356"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HBA7629517.1"
                     /translation="MSEFITVARPYAKAAFDFAVEHQSVERWQDMLAFAAEVTKNEQM
                     AELLSGALAPETLAESFIAVCGEQLDENGQNLIRVMAENGRLNALPDVLEQFIHLRAV
                     SEATAEVDVISAAALSEQQLAKISAAMEKRLSRKVKLNCKIDKSVMAGVIIRAGDMVI
                     DGSVRGRLERLADVLQS"
     gene            complement(13517..13987)
                     /gene="atpF"
                     /locus_tag="J1Y83_001357"
     CDS             complement(13517..13987)
                     /gene="atpF"
                     /locus_tag="J1Y83_001357"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001052215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HBA7629518.1"
                     /translation="MNLNATILGQAIAFVLFVLFCMKYVWPPLMAAIEKRQKEIADGL
                     ASAERAHKDLDLAKASATDQLKKAKAEAQVIIEQANKRRSQILDEAKAEAEQERTKIV
                     AQAQAEIEAERKRAREELRKQVAILAVAGAEKIIERSVDEAANSDIVDKLVAEL"
     gene            complement(14049..14288)
                     /gene="atpE"
                     /locus_tag="J1Y83_001358"
     CDS             complement(14049..14288)
                     /gene="atpE"
                     /locus_tag="J1Y83_001358"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004246596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HBA7629519.1"
                     /translation="MENLNMDLLYMAAAVMMGLAAIGAAIGIGILGGKFLEGAARQPD
                     LIPLLRTQFFIVMGLVDAIPMIAVGLGLYVMFAVA"
     gene            complement(14335..15150)
                     /gene="atpB"
                     /locus_tag="J1Y83_001359"
     CDS             complement(14335..15150)
                     /gene="atpB"
                     /locus_tag="J1Y83_001359"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HBA7629520.1"
                     /translation="MASENMTPQDYIGHHLNNLQLDLRTFSLVDPHNPPATFWTINID
                     SMFFSVVLGLLFLVLFRSVAKKATSGVPGKFQTAIELVIGFVNGSVKDMYHGKSKLIA
                     PLALTIFVWVFLMNLMDLLPIDLLPYIAEHVLGLPALRVVPSADVNVTLSMALGVFIL
                     ILFYSIKMKGIGGFTKELTLQPFNHWAFIPVNLILEGVSLLSKPVSLGLRLFGNMYAG
                     ELIFILIAGLLPWWSQWILNVPWAIFHILIITLQAFIFMVLTIVYLSMASEEH"
     gene            complement(15159..15539)
                     /gene="atpI"
                     /locus_tag="J1Y83_001360"
     CDS             complement(15159..15539)
                     /gene="atpI"
                     /locus_tag="J1Y83_001360"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418195.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit I"
                     /protein_id="HBA7629521.1"
                     /translation="MSVSLVSRNVARKLLLVQLLVVIASGLLFSLKDPFWGVSAISGG
                     LAVFLPNVLFMIFAWRHQAHTPAKGRVAWTFAFGEAFKVLAMLVLLVVALAVLKAVFL
                     PLIVTWVLVLVVQILAPAVINNKG"
     gene            complement(16156..16779)
                     /gene="rsmG"
                     /locus_tag="J1Y83_001361"
     CDS             complement(16156..16779)
                     /gene="rsmG"
                     /locus_tag="J1Y83_001361"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HBA7629522.1"
                     /translation="MLNKLSLLLKDAGISLTDHQKNQLIAYVNMLHKWNKAYNLTSVR
                     DPNEMLVRHILDSIVVAPYLQGERFIDVGTGPGLPGIPLSIVRPEAHFTLLDSLGKRV
                     RFLRQVQHELKLENIEPVQSRVEEFPSEPPFDGVISRAFASLNDMVSWCHHLPGEQGR
                     FYALKGQMPEDEIALLPEEYQVESVVKLQVPALDGERHLVVIKANKI"
     gene            complement(16843..18732)
                     /gene="mnmG"
                     /locus_tag="J1Y83_001362"
     CDS             complement(16843..18732)
                     /gene="mnmG"
                     /locus_tag="J1Y83_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000499793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HBA7629523.1"
                     /translation="MFYPDPFDVIIIGGGHAGTEAAMAAARIGQQTLLLTHNIDTLGQ
                     MSCNPAIGGIGKGHLVKEVDALGGLMAKAIDQAGIQFRILNASKGPAVRATRAQADRV
                     LYRQAVRTALENQPNLMIFQQAVEDLIVEKDRVVGAVTQMGLKFRAKAVVLTVGTFLD
                     GKIHIGLDNYSGGRAGDPPSIPLSRRLRELPLRVGRLKTGTPPRIDARTIDFSVLAQQ
                     HGDNPMPVFSFMGNASQHPQQVPCYITHTNEKTHDVIRSNLDRSPMYAGVIEGVGPRY
                     CPSIEDKVMRFADRNQHQIFLEPEGLTSNEIYPNGISTSLPFDVQMQIVRSMQGMENA
                     KIVRPGYAIEYDFFDPRDLKPTLESKFIQGLFFAGQINGTTGYEEAAAQGLLAGLNAA
                     RLSADKEGWAPARSQAYLGVLVDDLCTLGTKEPYRMFTSRAEYRLMLREDNADLRLTE
                     IGRELGLVDDERWARFNEKLENIERERQRLKSTWVTPSAEAAAEVNAHLTAPLSREAS
                     GEDLLRRPEMTYEKLTTLTPFAPALTDEQAAEQVEIQVKYEGYIARQQDEIEKQLRNE
                     NTLLPATLDYRQVSGLSNEVIAKLNDHKPASIGQASRISGVTPAAISILLVWLKKQGM
                     LRRSA"
     gene            complement(19111..19554)
                     /gene="mioC"
                     /locus_tag="J1Y83_001363"
     CDS             complement(19111..19554)
                     /gene="mioC"
                     /locus_tag="J1Y83_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein MioC"
                     /protein_id="HBA7629524.1"
                     /translation="MADITLISGSTLGGAEYVAEHLAEKLEEAGFTTETLHGPLLEDL
                     SASGIWLVISSTHGAGDIPDNLSPFYEALQEQKPDLSAVRFGAIGIGSREYDTFCGAI
                     DKLEAELKNSGAKQTGETLKINILDHDIPEDPAEEWLGSWINLLK"
     gene            complement(19644..20102)
                     /gene="asnC"
                     /locus_tag="J1Y83_001364"
     CDS             complement(19644..20102)
                     /gene="asnC"
                     /locus_tag="J1Y83_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator AsnC"
                     /protein_id="HBA7629525.1"
                     /translation="MENYLIDNLDRGILEALMGNARTAYAELAKQFGVSPGTIHVRVE
                     KMKQAGIITGARIDVSPKQLGYDVGCFIGIILKSAKDYPSALAKLESLDEVTEAYYTT
                     GHYSIFIKVMCRSIDALQHVLINKIQTIDEIQSTETLIVLQNPIMRTIKP"
     gene            20254..21246
                     /gene="asnA"
                     /locus_tag="J1Y83_001365"
     CDS             20254..21246
                     /gene="asnA"
                     /locus_tag="J1Y83_001365"
                     /EC_number="6.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--ammonia ligase"
                     /protein_id="HBA7629526.1"
                     /translation="MKTAYIAKQRQISFVKSHFSRQLEERLGLIEVQAPILSRVGDGT
                     QDNLSGCEKAVQVKVKALPDAQFEVVHSLAKWKRQTLGQHDFSAGEGLYTHMKALRPD
                     EDRLSPLHSVYVDQWDWERVMGDGERQFSTLKSTVEAIWAGIKATEAAVSEEFGLAPF
                     LPDQIHFVHSQELLSRYPDLDAKGRERAIAKDLGAVFLVGIGGKLSDGHRHDVRAPDY
                     DDWSTPSELGHAGLNGDILVWNPVLEDAFELSSMGIRVDADTLKHQLALTGDEDRLEL
                     EWHQALLRGEMPQTIGGGIGQSRLTMLLLQLPHIGQVQCGVWPAAVRESVPSLL"
     gene            complement(21251..22702)
                     /gene="viaA"
                     /locus_tag="J1Y83_001366"
     CDS             complement(21251..22702)
                     /gene="viaA"
                     /locus_tag="J1Y83_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase RavA stimulator ViaA"
                     /protein_id="HBA7629527.1"
                     /translation="MLTLDTLNVMLAVSEEGLIEEMIIALLASPQLAVFFEKFPRLKA
                     AITDDVPRWREALRSRLKDARVPPELTEEVMCYQQSQLLSTPQFIVQLPQILDLLHRL
                     NSPWAEQARQLVDANSTITSALHTLFLQRWRLSLIVQATTLNQQLLEEEREQLLSEVQ
                     ERMTLSGQLEPILADNNTAAGRLWDMSAGQLKRGDYQLIVKYGEFLNEQPELKRLAEQ
                     LGRSREAKSIPRNDAQMETFRTMVREPATVPEQVDGLQQSDDILRLLPPELATLGITE
                     LEYEFYRRLVEKQLLTYRLHGESWREKMIERPVVHKDYDEQPRGPFIVCVDTSGSMGG
                     FNEQCAKAFCLALMRIALAENRRCYIMLFSTEIVRYELSGPQGIEQAIRFLSQQFRGG
                     TDLASCFRAIMERLQSREWFDADAVVISDFIAQRLPDDVTSKVKELQRIHQHRFHAVA
                     MSAHGKPGIMRIFDHIWRFDTGMRSRLLRRWRR"
     gene            complement(22696..24192)
                     /gene="ravA"
                     /locus_tag="J1Y83_001367"
     CDS             complement(22696..24192)
                     /gene="ravA"
                     /locus_tag="J1Y83_001367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312715.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase RavA"
                     /protein_id="HBA7629528.1"
                     /translation="MAHPHLLAERISRLSSSLEKGLYERSHAIRLCLLAALSGESVFL
                     LGPPGIAKSLIARRLKFAFQNARAFEYLMTRFSTPEEVFGPLSIQALKDEGRYERLTS
                     GYLPEAEIVFLDEIWKAGPAILNTLLTAINERQFRNGALVEKIPMRLLVAASNELPEA
                     DSSLEALYDRMLIRLWLDKVQDKANFRSMLTSQQDENDNPVPASLQITDEEYERWQKE
                     IGEITLPDHVFELIFMLRQQLDKLPDAPYVSDRRWKKAIRLLQASAFFSGRSAVAPVD
                     LILLKDCLWYDAQSLNLIQQQIDVLMTGHAWQQQGMLTRLGAIVQRHLQLQQQQSDKT
                     ALTVIRLGGIFSRRQQYQLPVNVTASTLTLLLQKPLKLHDMEVVHISFERSALEQWLS
                     KGGEIRGKLNGIGFAQKLNLEVDSAQHLVVRDVSLQGSTLALPGSSAEGLPSEIKQQL
                     EELESDWRKQHALFSEQQKCLFIPGDWLGRIEASLQDVGAQIRQAQQC"
     gene            24415..26283
                     /gene="kup"
                     /locus_tag="J1Y83_001368"
     CDS             24415..26283
                     /gene="kup"
                     /locus_tag="J1Y83_001368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low affinity potassium transporter Kup"
                     /protein_id="HBA7629529.1"
                     /translation="MSTDNKQSLPAITLAAIGVVYGDIGTSPLYTLRECLSGQFGFGV
                     ERDAVFGFLSLIFWLLIFVVSIKYLTFVMRADNAGEGGILTLMSLAGRNTSARTTSML
                     VIMGLIGGSFFYGEVVITPAISVMSAIEGLEIVAPQLDTWIVPLSIIVLTLLFMIQKH
                     GTAMVGKLFAPIMLTWFLILAGLGLRSIIANPEVLHALNPMWAVHFFLEYKTVSFIAL
                     GAVVLSITGVEALYADMGHFGKFPIRLAWFTVVLPSLTLNYFGQGALLLKNPEAIKNP
                     FFLLAPDWALIPLLIIAALATVIASQAVISGVFSLTRQAVRLGYLSPMRIIHTSEMES
                     GQIYIPFVNWMLYVAVVIVIVSFEHSSNLAAAYGIAVTGTMVLTSILSTTVARQNWHW
                     NKYFVALILIAFLCVDIPLFTANLDKLLSGGWLPLSLGTVMFIVMTTWKSERFRLLRR
                     MHEHGNSLEAMIASLEKSPPVRVPGTAVYMSRAINVIPFALMHNLKHNKVLHERVILL
                     TLRTEDAPYVHNVRRVQIEQLSPTFWRVVASYGWRETPNVEEVFHRCGLEGLSCRMME
                     TSFFMSHESLILGKRPWYLRLRGKLYLLLQRNALRAPDQFEIPPNRVIELGTQVEI"
     gene            26450..26869
                     /gene="rbsD"
                     /locus_tag="J1Y83_001369"
     CDS             26450..26869
                     /gene="rbsD"
                     /locus_tag="J1Y83_001369"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709562.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HBA7629530.1"
                     /translation="MKKGTVLNSDISSVISLLGHTDTLVVCDAGLPIPKSTTRIDMAL
                     TQGVPSFMQVLGVVTNEMQVEAVIIAEEIKQHNPQLHETLLTHLEQLQQHQGNTIEIR
                     YTTHEQFKQQTAESQAVIRSGECSPYANIILCAGVTF"
     gene            26877..28382
                     /gene="rbsA"
                     /locus_tag="J1Y83_001370"
     CDS             26877..28382
                     /gene="rbsA"
                     /locus_tag="J1Y83_001370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter ATP-binding protein RbsA"
                     /protein_id="HBA7629531.1"
                     /translation="MEALLQLKGIDKAFPGVKALSGAALNVYPGRVMALVGENGAGKS
                     TMMKVLTGIYTRDAGTLLWLGKETTFTGPKSSQEAGIGIIHQELNLIPQLTIAENIFL
                     GREFVNRFGKIDWKTMYAEADKLLAKLNLRFKSDKLVGDLSIGDQQMVEIAKVLSFES
                     KVIIMDEPTDALTDTETESLFRVIRELKSQGRGIVYISHRMKEIFEICDDVTVFRDGQ
                     FIAEREVASLTEDSLIEMMVGRKLEDQYPHLNKAPGDIRLKVDNLCGPGVNDVSFTLR
                     KGEILGVSGLMGAGRTELMKVLYGALPRTSGYVTLDGHEVVTRSPQDGLANGIVYISE
                     DRKRDGLVLGMSVKENMSLTALRYFSRAGGSLKHADEQQAVSDFIRLFNVKTPSMEQA
                     IGLLSGGNQQKVAIARGLMTRPKVLILDEPTRGVDVGAKKEIYQLINQFKADGLSIIL
                     VSSEMPEVLGMSDRIIVMHEGHLSGEFTREQATQEVLMAAAVGKLNRVNQE"
     gene            28387..29352
                     /gene="rbsC"
                     /locus_tag="J1Y83_001371"
     CDS             28387..29352
                     /gene="rbsC"
                     /locus_tag="J1Y83_001371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002882531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter permease"
                     /protein_id="HBA7629532.1"
                     /translation="MTTQTVSGRRYFTKAWLMEQKSLIALLVLIAIVSTLSPNFFTIN
                     NLFNILQQTSVNAIMAVGMTLVILTSGIDLSVGSLLALTGAVAASIVGIEVNALVAVA
                     AALALGAAIGAVTGVIVAKGRVQAFIATLVMMLLLRGVTMVYTNGSPVNTGFTENADL
                     FGWFGIGRPLGVPTPVWIMGIVFLAAWYMLHHTRLGRYIYALGGNEAATRLSGINVNK
                     IKIIVYSLCGLLASLAGIIEVARLSSAQPTAGTGYELDAIAAVVLGGTSLAGGKGRIV
                     GTLIGALILGFLNNGLNLLSVSSYYQMIVKAVVILLAVLVDNKKQ"
     gene            29377..30267
                     /gene="rbsB"
                     /locus_tag="J1Y83_001372"
     CDS             29377..30267
                     /gene="rbsB"
                     /locus_tag="J1Y83_001372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter substrate-binding protein
                     RbsB"
                     /protein_id="HBA7629533.1"
                     /translation="MNMKKLATLVSAVALSATVSANAMAKDTIALVVSTLNNPFFVSL
                     KDGAQKEADKLGYNLVVLDSQNNPAKELANVQDLTVRGTKILLINPTDSDAVGNAVKM
                     ANQANIPVITLDRQATKGEVVSHIASDNVLGGKIAGDYIAKKAGEGAKVIELQGIAGT
                     SAARERGEGFQQAVAAHKFNVLASQPADFDRTKGLNVMQNLLTAHPDVQAVFAQNDEM
                     ALGALRALQTAGKSDVMVVGFDGTPDGEKAVNDGKLAATIAQLPDQIGAKGVETADKV
                     LKGEKVQAKYPVDLKLVVKQ"
     gene            30393..31322
                     /gene="rbsK"
                     /locus_tag="J1Y83_001373"
     CDS             30393..31322
                     /gene="rbsK"
                     /locus_tag="J1Y83_001373"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HBA7629534.1"
                     /translation="MQNAGSLVVLGSINADHILNLQSFPTPGETVTGNHYQVAFGGKG
                     ANQAVAAGRSGANIAFIACTGDDSIGESVRQQLATDNIDISPVSVIKGESTGVALIFV
                     NGEGENVIGIHAGANAALSPALVEAQRERIANASALLMQLESPLESVMAAAKIAHQNK
                     TIVALNPAPARELPDELLALVDIITPNETEAEKLTGIRVENDEDAAKAAQVLHEKGIR
                     TVLITLGSRGVWASVNGEGQRVPGFRVQAVDTIAAGDTFNGALITALLEEKPLPEAIR
                     FAHAAAAIAVTRKGAQPSVPWREEIDAFLDRQR"
     gene            31326..32318
                     /gene="rbsR"
                     /locus_tag="J1Y83_001374"
     CDS             31326..32318
                     /gene="rbsR"
                     /locus_tag="J1Y83_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose operon transcriptional repressor RbsR"
                     /protein_id="HBA7629535.1"
                     /translation="MATMKDVARLAGVSTSTVSHVINKDRFVSEAITAKVEAAIKELN
                     YAPSALARSLKLNQTHTIGMLITASTNPFYSELVRGVERSCFERGYSLVLCNTEGDEQ
                     RMNRNLETLMQKRVDGLLLLCTETHQPSREIMQRYPTVPTVMMDWAPFDGDSDLIQDN
                     SLLGGDLATQYLIDKGHTRIACITGPLDKTPARLRLEGYRAAMKRAGLNIPDGYEVTG
                     DFEFNGGFDAMRQLLSHPLRPQAVFTGNDAMAVGVYQALYQAELQVPQDIAVIGYDDI
                     ELASFMTPPLTTIHQPKDELGELAIDVLIHRITQPALQQQRLQLTPILMERGSA"
     gene            complement(32284..33711)
                     /locus_tag="J1Y83_001375"
     CDS             complement(32284..33711)
                     /locus_tag="J1Y83_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHA2 family efflux MFS transporter permease
                     subunit"
                     /protein_id="HBA7629536.1"
                     /translation="MSDKKKRSMAGLPWIAAMAFFMQALDATILNTALPAIAHSLNRS
                     PLAMQSAIISYTLTVAMLIPVSGWLADRFGTRRIFTLAVSLFTLGSLACALSNSLPQL
                     VVFRVIQGIGGAMMMPVARLALLRAYPRNELLPVLNFVAMPGLVGPILGPVLGGVLVT
                     WATWHWIFLINIPIGIAGLLYARKHMPNFTTARRRFDTTGFLLFGLSLVLFSSGIELF
                     GEKIVASWIALTVIVTSIWLLLLYILHARRTPNPLISLDLFKTRTFSIGIVGNIATRL
                     GTGCVPFLMPLMLQVGFGYQAFIAGCMMAPTALGSIIAKSMVTQVLRRLGYRHTLVGI
                     TVIIGLMIAQFSLQSPAMAIWMLILPLFILGMAMSTQFTAMNTITLADLTDDNASSGN
                     SVLAVTQQLSISLGVAVSAAVLRVYEGMEGTTTVEQFHYTFITMGIITVASAAMFMLL
                     KTTDGNNLIKRQRKSKPNHVPSESE"
     gene            complement(33734..34426)
                     /locus_tag="J1Y83_001376"
     CDS             complement(33734..34426)
                     /locus_tag="J1Y83_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FadR family transcriptional regulator"
                     /protein_id="HBA7629537.1"
                     /translation="MPLSAQQLAAQKNLSYVLAEKLAQRILKGEYEPGTILPGEIELG
                     EQFGVSRTAVREAVKTLTAKGMVLPRPRIGTRVMPQSNWNFLDQELLTWWMTEENFHQ
                     VIDHFLVMRICLEPQACLLAAKVGTAEQKAHLNTLMAEMAALKENFRRERWIEVDMAW
                     HEHIYEMSANPFLTSFASLFHSVYHTYFTSITSDTVIKLDLHQAIVDAIIQSDGDAAF
                     KACQALLRSPDK"
BASE COUNT         8333 a         8927 c         8798 g         8487 t
ORIGIN      
        1 ataacaaatc ccaataatta aattattggg atttacttgg tgaattattt atcgctatct
       61 ttttctgcca gcagtttatc gagctcgtca ccaccgacgt gacggaaatc ctgccccttc
      121 acgtagtaga agataaactc acaaatattc tggcagcggt cgccaatacg ttcgatagaa
      181 cgcgcacaga ataacgcggt aagtacgctc ggaatggtac gcgaatcttc catcatgtag
      241 gtcatcagtt gacgaacaat accttcgtac tcctgatcga cttttttatc ttcacgataa
      301 atacgtaccg cttcgtcaat gtccatccgc gcgaacgcgt ccagcacgtc gtgcagcatc
      361 tggatggtat gacggcccag cgactccaga cttaccagca atggctgatg ctgctgagag
      421 aatttctcca gtgcagtacg gcagattttg tccgccacgt cgccaatacg ctccagctcg
      481 gcaatggttt tactgatcac cataaccaga cgcaggtcgc tcgccgtcgg ctgacgtttg
      541 gcgataatgc gcacgcaggc ttcatcgatc gccacttcca tcatgttgac gttcttgtcg
      601 ccttcgatga cgcgcttcgc cagatcgctg tcctggttat gcatcgcggt gattgcatca
      661 gaaagctgct gctccaccat gccgcccatg gtcatcacct gcgtgcggat gctttccagt
      721 tcggcgttga actggccgga aatatgttta ttaagattga gactgtccat aacgcactcc
      781 tgaatcaacc gtaacgaccg gtgatgtagt cttctgtttg tttcttcgct ggcttggtga
      841 acagatcgtc cgtgttgctg aactcaatca attcgcccag gtacataaac gccgtgtggt
      901 cggaacaacg cgcagcctgc tgcatgttgt gggtgacgat caccacggtg taatcctgct
      961 tcagttcggt gatcagctct tcaatacgcc cggtagagat ggggtcgagt gccgaacacg
     1021 gttcgtcgag cagcagcact tccgggcgaa tggcaatacc acgcgcaata cacaaacgct
     1081 gttgctgacc accagagaga gagtaaccgc tctggtgcaa tttatctttg gtttcgttcc
     1141 acaatgcggc tttggtcaat gcccactgca cgcgctcgtc catgtcagca cgggagagct
     1201 tctcaaacag acgcacgcca aaagcgatgt tgtcgtagat ggacatcggg aacggcgtcg
     1261 gtttctggaa caccatgccc actttcgcac gcagcagggc gatatcctga gagttggtca
     1321 ggatgttgtc gccatcgagc agaatttcac cttccgcacg ctgttccggg tacagttcaa
     1381 acattttgtt gaaggtacgc agcagcgttg atttaccgca gccggacggc ccgataaacg
     1441 ccgtaacctg gtttttggcg atatccaggt tgatgttttt cagggcatgg aatttgccgt
     1501 agtagaagtt caaattacga acctgaattt tactcggggc agtttcaacc atactcattg
     1561 caatcttttc ctcatcgtgc ctgatgcact tcgcgaatca ggcttaccgt aggccggata
     1621 aggcgtttac gccgcatcca gcaaagtgtt aaccgtgttt attcttcgca aaaacaacgc
     1681 gcgccagaat gttcagcagc agtacgcaca gggtaatgat caacaccccg gcccaggcca
     1741 gttgctgcca ttccgcaaac gggctcatcg caaacttaaa gatcgtcacc ggcaggttgg
     1801 cgatcggctg catcatgtcc gtgctccaga actggttgga gagcgcagta aacagcagcg
     1861 gtgcggtttc accagcaata cgggcaatcg ccagcaggat accggtcatg atcccggaca
     1921 ccgacgcttt cagcgtgatc gaggcgatca tcttccactt cggcgtaccc agcgcataag
     1981 ccgcttcacg caggctgtcc ggcaccagtt tcagcatgtt ctcggtggtg cggataacaa
     2041 tcggcacctg caacagcgcc agcgcaatca cgcccgccca gccggagaag tgctccatct
     2101 gcgccaccac gatggtgtaa acaaaaagac caaccacaat cgacggtgcg gagagcaaaa
     2161 tgtcgttaat gaagcgaatc acttctgcca gccaggattt acgaccatat tcagccagat
     2221 aaatccccgc cataatgccc agcggcgtac cgaataccgt ggcccacaaa attaacaggc
     2281 cgctacccgc cagggcgttc gccagaccac cgccttccgt atttggcggc ggcgtcattt
     2341 cggtgaacag cgccagcgac ataccatcga taccgcgagt gatggtggac attaaaatcc
     2401 agatcagcca gaacagaccg aaggccatcg tcgccatcga gagcgtcagc gcaatacggt
     2461 tcttaaggcg gcgacgcgcc tgcattttac ggcgggattc agccagcgcc gcagtggttt
     2521 gcatttcaac catagccatt agcgtgcccc ctcattctta gccaggcgca taatcataaa
     2581 cttcgatgcg gcgaggacga tgaaggtaat cacaaacagg atcaggccca gttccatcag
     2641 tgcagcaacg tgcagacccg actctgcttc cgcaaattcg ttggccagcg cagaggtgat
     2701 actgttgccc ggcatgtata gcgaggcgct gtcgagctgg taggtgttac cgatgataaa
     2761 ggtcaccgcc atggtttcac cgagcgcgcg ccccagcccc aacataatgc cgccgataac
     2821 accatttttg gtgaacggaa gaacgatacg ccagataact tcccaggtgg tgcagccgat
     2881 accgtaggct gactctttca tcatcaccgg ggtttgttcg aatacatcgc gcattaccgc
     2941 cgcaatgtac ggaataatca tgatggcgag gatcacgcct gccgcgagga taccgatacc
     3001 aaaggccggg ccggagaaca gcgcgccgac aatcgggata ttcgacatga tattgccgac
     3061 cggctcctgg aagtaaacgg cgaacagcgg cgcaaagata aacaggcccc acatgccgta
     3121 aacgatactt ggaatggcag ccagcagctc aatggcgata cccagcgggc gtttcagcca
     3181 gccaggcgca agctcggtca ggaacagggc gataccaaaa ctcaccggga cggcgatcag
     3241 cagcgcgata aacgaggtca ccagcgtacc gtagatcggc accagcgccc cgtagatatc
     3301 gttcggcgca tcccactctt tggtccacag gaaagccaga ccaaatttct gaatgctcgg
     3361 ccaggatgag atgatcagag agacaataat gccacccaac atcaatagca caatcagcgc
     3421 cgccagtttt accagcacgc tgaaaattat gtcgcccttt ttacccggtg ggttaaaagc
     3481 aggcttggtt gcagccataa gttactcttc agttaaacgc gtttacaaag tcattttgta
     3541 atgccggatg cggcgtaaaa caccgtaccc ggcctggagt tttattagta cagcggcttg
     3601 ccgctactgt ctttaatatt ggtcttccac gcagcgcgaa cctgttcaac tacgctatcc
     3661 ggcaggctgg cataatccag gtcgttcgcc tgtttagccc cggttttgta tgcccagtcg
     3721 aagaacttca gcacttcggt gccttgttct ggtttcttct gatctttatg gatcagaatg
     3781 aacgtggtag aggtaatagg ccatgcatct tcgcctttct ggttggtcag atcctgagcg
     3841 aatgttttgc tccagtctgc gccttttgct gcattagcga agttttcttc ggtcggactc
     3901 accggtttgc catcagcgga gatcagtttg gtgtacgcca ggttgttctg cttcgcgtaa
     3961 gcatattcaa cgtaaccaat tgcacccggc agacgctgaa cgaacgcggc gataccgtcg
     4021 ttacctttac cgcccagacc gatcggccat tttacggtag agccagtacc aacgttgttt
     4081 ttccactctt cgttcacttt cgccaggtag ctggtgaaga cgaaggaagt cccggagcca
     4141 tctgcgcggc gtactacagc aatgttctgt gaaggcagtt tcagacccgg attcagtttg
     4201 gcgatggctt catcatccca cttcttgatt ttgcccaggt agatgtcgcc gagggtttta
     4261 ccatccagca ccagttcgcc agacttcaga cctggaatat taaccgccag caccacgccg
     4321 ccaatcacgg tcgggaactg gaacaggcct tcctgagcca gtttttcgtc agacagcggc
     4381 gcgtcagaag caccaaaatc aacggtattg gcgatgatct gttttacgcc accagaagaa
     4441 ccgataccct ggtagttaac tttattaccg gtttctttct ggtaagtgtc agcccatttg
     4501 gcatacaccg gcgcagggaa ggttgcacct gcacctgtca ggcttgcttc tgcaaacaca
     4561 gagaaagcac tcatcgataa ggtcgcggcg acaacagttg cgacggtggt acgcataact
     4621 ttcataatgt ctcctgggag gattcataaa gcattgtttg ttggctacga gaagcaaaat
     4681 aggacaaaca ggtgacagtt atatgtaagg tttatgacag ttttatgaca gagagagaaa
     4741 gtctttagtc tgatttaaat aagcgttgat attcagttaa ttacaaatat taataacgaa
     4801 gagatgacag aaaattttca ttctgtgaca gagaaaaagt agccgaagat gacggtttgt
     4861 cacatggagt tggcaggatg tttgattaaa aacataacag gaagaaaaat gccccgctta
     4921 cgcagggcat ccatttatta ctcaaccgtt accgattttg ccaggttacg cggctggtca
     4981 acgtcggtgc ctttgatcag cgcaacgtgg taagccagta gttgcagcgg aacggtgtag
     5041 aagatcggtg caatcacctc ttccacatgc ggcatctcaa taatgtgcat attgtcactg
     5101 ctcacaaaac ccgcatcctg atcggcgaag acatacaact gaccgccacg cgcgcgaact
     5161 tcttcaatgt tggatttcag tttttccagc aattcgttgt tcggtgcaac gacgataacc
     5221 ggcatatcgg catcaatcag cgccagcgga ccgtgtttca gttcgcctgc agcgtaggct
     5281 tcagcgtgaa tgtaagagat ctctttcagc ttcaatgcac cttccagcgc gattgggtac
     5341 tgatcgccac ggcccaggaa cagcgcgtga tgtttgtcag agaaatcttc tgccagcgct
     5401 tcaatgcgtt tgtcctgaga cagcatctgc tcaatacggc tcggcagcgc ctgcagacca
     5461 tgcacgatgt catgttcaat ggaggcatcc agacctttca ggcgagacag tttcgccacc
     5521 agcatcaaca gcacagttaa ctgggtggtg aatgctttgg tggaggccac gccaatttcc
     5581 gtaccggcgt gggtcatcag cgccagatcg gattcgcgca ccagagaaga acccggaacg
     5641 ttacagattg ccagcgaacc aaggtaaccc agctctttcg acagacgcag gcccgccagg
     5701 gtatccgcgg tttcgccaga ctgtgacaag gtgatcatca ggctgttacg acgcacggca
     5761 gatttgcgat agcggaattc agaggcgatt tcgacgtcgc acggaatacc tgccagcgat
     5821 tcaaaccagt agcgggaaac cataccggag ttataagaag taccacaggc gaggatctga
     5881 atatgctcaa ccttcgacag cagttcatcg gcgttcggtc ccagctcgct taaatcaacc
     5941 tgaccgtggc tgatgcgccc ggtaagggtg tttttgatcg cgttcggctg ttcgtagatc
     6001 tctttctgca tgtagtggcg gtaaatacct ttatcgcccg cgtcatattg cagattggat
     6061 tcgatatcct gacgttttac ttccgcgcca gttttatcga agatgtttac cgaacggcga
     6121 gtgatttccg caatatcgcc ctcttcaagg aagataaaac gacgagtcac tggcaacagc
     6181 gccagctggt cagaagcgat aaagttttcg cccataccca ggccaatcac cagcggacta
     6241 ccagaacgtg ccgccagcag ggtatccgga tgacgggagt ccatgatcac tgtaccgtac
     6301 gcaccacgca gttgcgggat agcacgcaga actgcctcac gcagagtacc gccttgtttc
     6361 agctcccagt tcaccaaatg tgcaatcact tcggtgtcgg tttcagaaac gaaggtatag
     6421 ccacgcgctt ttaactcttc acgcagcggt tcatggtttt cgatgatgcc gttatgcacc
     6481 accacaatgt gttcagaaac atgcggatgc gcgttagctt ctgaaggttc accatgggtc
     6541 gcccagcgag tgtgagcaat accggtgccg ccatgcagag gatgttcttc cgctgcctga
     6601 gccagcatct gtactttacc gaggcgacgc aggcgggtca tatgaccttc tgcatcaaca
     6661 acggccagac cggcagaatc atatccgcgg tattccagac gacgtaaacc ttcaagaagg
     6721 atttctgcta catcacgttg cgcgatcgcg ccaacaattc cacacatagt ttttgattcc
     6781 gatttatatc gttgtcggtc aacctgtatg cccgtttttc gggcgccccg agccttgtag
     6841 agagtggggt tatttttata gttactgctt gtgggcggga gattatgtta tctcctcatc
     6901 ccatatgacc ggattacccg gccagaatta ctttttcttt accggacgac gccagccttc
     6961 tttttgagtc tgcggcacac ggctgatcgc cagggcattt tcgccgacat tacgcgtcac
     7021 agttgtaccc gcagcaatgg tcgcgccttt gcctactgtt accggggcca ccagttgagt
     7081 gtcggaaccg acaaacacat cgtcgccgat aatggtctta aatttattcg caccatcgta
     7141 gttgcaggta atggttcccg cgccgatgtt aacgttatcg ccaatttccg catcgcccag
     7201 gtaagtcaga tgaccagctt tcgagccttt acccagacgc gcttttttca tctcaacgaa
     7261 gttaccgacg tgtgcacctt ccagcaactc agcaccagga cgcagacggg caaacgggcc
     7321 aatagtacag gccgctgcca gattcgcatc ttccacgacg gtatacggac tgatttcgca
     7381 atcatcgcca atcacgctgt ttttaatcac gcaaccggtg ccgattttca cgcgatgacc
     7441 gagagtcacg ttgccctcga tgataacgtt agtatcaatt tcaacatcgc gcccgtgagt
     7501 aagcgtaccg cgcagatcaa aacgcgctgg atcgcgcagc ataacgcctg ctaacagcag
     7561 tttttcagcc tgttcggact ggtaaacgcg ctccagtcgg gagagttgca ggcggttatt
     7621 cacgccttct acttcgctta aacgttgcgg atgaacggcg acgatttcac gtccttcctg
     7681 atacgccagc gcaataatgt cggtgatgta gtattcgccc tgagcattat tgttggtcag
     7741 cttcgccagc cagcgtttca tatctgcgcc gttggcaatc agaatgccgg tgttgatctc
     7801 ctgaatctga cgctgctcgt cggtggcgtc tttgtgctca acaatgccgg taactttgcc
     7861 gttttcacgg gtgatacgtc cataaccggt cggatcctcc agttttaccg tcagcagacc
     7921 aatgccaccc tgcggtttag catcacgcag gcgctggagt gtttcgacag agatcagcgg
     7981 cacgtcgccg tagagcatta aaatgtcttc atcatcggca aagaaaggtg cggcctgctg
     8041 catcgcatga cctgtaccca gctgttctgc ctgaagcacc cagttcaggt tgtcatcttt
     8101 cagcgcctgt ttaagcagat cgccgccgtg accgtaaacc aggtgaacgt gcgctgcgcc
     8161 taattcattc gcagcatcaa tgacatgctg aaccatcgct ttcccggcaa gggtatgcag
     8221 cactttcgga agatcggaat acatgcgcgt gcctttgcct gcggcaagga tcactacgct
     8281 catagcatta ttcaacatac gcgtcctgac tgtaatttga gaacgaattt aaaccgcttc
     8341 accttgaaaa aactacattt ttttcatcgt gaaatggaca gaggataaat tgttcaataa
     8401 cggattatcc ccgctgacga aagcgccatt ttcgaccatc gtcacgcctt ttgtctgcag
     8461 aaaattaagg cagtaccttc atttaagcgt caggtggatg tttttgctct tatttcgatc
     8521 aatgaataaa cgggaaaaca ggggtgtttt atatcatcag gatctatgtg aacgcttttc
     8581 aggacgggtc aatacgcgta aaaaaaagcc agcctgtttc cagactggct tttgtgcttt
     8641 tcaagccggt gttacatcgc ttttttggtc aactcgataa cgcgcagctg cgcgatcgct
     8701 ttggccagtt ccgcagacgc ctgagcgtaa tctacgtcgc cgtgagagct gctaatgtgc
     8761 tcttcagcct tacgtttcgc ttccatggct cgcgcttcgt cgagatcctg gccgcgaatt
     8821 gcggtgtcgg ccagaacggt cacgttgcca ggctgcactt caagaatgcc gccagacaga
     8881 tagataaact cttcgtgacc gtgctgtttc acgatgcgaa tcataccagg cttaatggcg
     8941 gtgagcagcg gtgcgtggcc agggtagatc cccagttcac cttcgctacc cgttacctgg
     9001 attttctcga ccagaccaga gaacatttgt tgctctgcgc tgacgacgtc caggtggtaa
     9061 gtcattgcca tatcaccctc cgattaaggc gttaaagttt tttggctttt tccacagctt
     9121 cttcgatgga accgaccatg tagaacgcct gctccggcag gtgatcgtat tcgccttcca
     9181 tgatgccttt aaagccacgg atggtgtctt tcagggagac gtatttaccc ggagaaccgg
     9241 tgaatacctc tgccacgaag aacggctggg acaggaagcg ctggatctta cgagcacgcg
     9301 ctaccaccag tttgtcttct tcagacagtt catccatacc caggatggcg ataatgtctt
     9361 tcagttcctg ataacgttgc aggatggact gaacgccacg cgcggtgtcg tagtgttcct
     9421 gaccaaccac cagcgggtcc agctgacggc tggtggagtc cagcgggtca acggccgggt
     9481 agatacccag agacgcgatc tgacggctca gtaccacggt tgcgtcaagg tgcgcaaagg
     9541 tggttgccgg agacgggtca gtcaagtcat ccgcaggtac gtatactgcc tgtacggagg
     9601 tgatagaacc ggttttggtg gaggtgatac gttcctgcag aacgcccatc tcttccgcca
     9661 gggtcggctg ataacctacc gctgaaggca tacggcccag cagtgcggat acttccgtac
     9721 cggccagggt gtaacgatag atgttgtcaa cgaacagcag aacgtcacga ccttcgtcac
     9781 ggaatttctc agccatggtc agaccggtca gagcaacgcg cagacggttt cccggcggct
     9841 cgttcatctg gccatacacc agggatactt tgtcgataac gttggagtcg gtcatttcgt
     9901 ggtagaagtc gttaccctca cgagtacgtt cacctacgcc cgcaaacaca gagtaaccgg
     9961 agtgctcgat cgcgatgtta cgaataagct ccatcatgtt tacggtttta cctacacccg
    10021 caccaccgaa cagaccaact ttaccgccct tagcgaacgg acacatcagg tcgataactt
    10081 tgataccggt ttccagcagt tcctgagagt ttgacagctc ttcgtaggaa ggtgctgcgc
    10141 gatgaatcgc ccaacgctct tcttcaccga tctcgccttt catgtcgacc ggttcaccca
    10201 gtacgttcat gatacgaccc agagtcgctt tacctaccgg gacttcgatc gggtgttcga
    10261 ggtcttttac atccagaccg cgacgcagac cgtcggagga acccattgcg atggtacgca
    10321 cgataccgcc gccgagctgc tgctgaactt ccagcaccag acgctcatta ccattttgca
    10381 cctcaagagc atcgtacacg cgcggtacgg catcctgagg gaattcgacg tcaactacgg
    10441 cgccgattac ctggacaatc tttccagtag ccatcttaaa tcctctacga aataacctgt
    10501 ttaaaccgcg gcggcccccg agacgatctc ggtgagttcc tgagtaatgc tggcctgacg
    10561 agctttgttg tataccaact gcagctcttt aatcaggctg ccgccattgt cggtcgcggc
    10621 tttcatcgcc accatacggg cggcctgctc gctggccagg ttttcaacca cgccctgata
    10681 aacctgagat tcgacataac gacgcagcag ggtatccagc aacgccttcg gatcgggttc
    10741 gtacaggtaa tcccaggatt tatgtttcag atcatcatca tctgatgccg gtaacggcag
    10801 cagctggctg atggtcggaa cctgagacat ggtgttaata aatttgttgc tgacaatgta
    10861 aagcttgtcc agacggcctt cgtcgtaggc ctgcaacatc acttttaccg gaccgatcag
    10921 ttcggacaag gaagggttat cccccatgcc ggtgacctgg gcaacaacat tgccgcccac
    10981 ggagttgaag aacgacacgc ctttcgagcc gatcattgcg aggtcgcatt gaacgccttt
    11041 gtcggtccag gtcttcattt ccgccagcag ttttttgaac aggttaatgt tcaaaccacc
    11101 gcacaaacca cggtcggtcg acaccaccag gtagcccacg cgtttaacgt cgcggtcttc
    11161 caggtaaggg tgcttatatt ccagattacc gtgtgcaagg tgaccaatca ctttgcgcat
    11221 ggtttctgca taaggacggc tggccgccat gcgatcctgc gatttacgca ttttggaagc
    11281 ggcgaccatc tccatcgctt tagtgatctt ttgcgtgttc tggacgcttg cgatcttact
    11341 acgtatctct tttgcgccgg ccatgagctt ctcctcaatg ccttgcggcc tgcccaaagg
    11401 caagccgcca gacgttacca ggattgggtt gctttgaagg aatcgaggat gcctttcagc
    11461 ttgccttcga tttcgtcgtt gtagccaccg gtctggttga tctcttgcat caacggagcg
    11521 tgatcacggt cgacgtaagc caacagagcg gcttcgaagc tgccgatttt cgacagttca
    11581 acatccgcca ggtaaccacg ttctgctgcg aacagaacca gagactgttg cgcaacagac
    11641 atcggcgcat actgtttctg cttcagcagt tcggtcactt tctgaccgtg gtcaagctgc
    11701 ttacgtgttg catcgtcaag gtcggatgca aactgagaga acgctgccag ttcacgatac
    11761 tgtgccagag cggtacggat accaccggac agttttttca tgatcttggt ctgtgctgca
    11821 ccaccaacac gggatacgga aatacccggg ttaaccgcag gacgaatacc ggcgttgaac
    11881 aggttggttt ccaggaagat ctgaccatcg gtaatggaga ttacgttggt cggaacgaac
    11941 gcagaaacgt cacccgcctg agtttcgata atcggcagcg cagtcagaga accggttttc
    12001 cctttcactt cacctttggt gaaagcttca acgtattcgg cgttaacacg cgcagcacgc
    12061 tccagcagac gagagtggag gtagaatacg tcgcccggga atgcttcacg tcctggcgga
    12121 cgacggagca gcagggagat ctgacggtaa gcaacagcct gtttagacag gtcatcgtaa
    12181 atgatcagcg catcttcacc gcggtcacgg aagtattcac ccattgcgca accagcgtac
    12241 ggtgccaggt attgcagtgc tgcggattca gacgcggttg ctaccacaac gatggtgtta
    12301 gccagtgcgc cgtgctcttc cagtttacgt accacgttag aaatggtgga cgctttctgg
    12361 ccgatagcga catagataca tttgataccg gaatcgcgct ggttgatgat ggcatcgata
    12421 gccagtgcgg ttttaccggt ctgacggtca ccgatgatca attcacgctg accacgaccg
    12481 attgggatca tggagtcaac ggctttataa ccggtctgta ccggctgatc tacggactga
    12541 cgttcgataa cgcccggagc gattgcttct acagcagaga agccgtcgtg atccagcgga
    12601 cctttaccgt cgattggtgc acccagagtg ttaaccacac ggcccagcag gccacggcca
    12661 accggaactt ccaggatacg gccagtacac ttaactttca tgccttcggc aaggtcagcg
    12721 tacggaccca taacaaccgc acctacagag tcgcgctcga ggttcagtgc gatagcgtaa
    12781 cggttacccg gcagggagat catttcaccc tgcatacaat cggccaggcc gtgaatgcgg
    12841 ataacaccgt cacttacaga aacaatagta ccttcgttgt gagcttcact cacaacattg
    12901 aactgagcaa tgcgctgctt gatcagttcg ctgatttcgg tggaattcag ttgcatgctc
    12961 cagtcccctt aagactgcaa gacgtctgca aggcgctcaa gacgaccgcg tacgctgcca
    13021 tcaatgacca tatcacccgc tcggatgata acgcctgcca ttacagactt atcgattttg
    13081 caattcagct taactttgcg tgacagacgt ttttccatcg cagcagaaat tttcgcgagc
    13141 tgttgttcac tcagtgcggc agcggaaatg acgtctacct cagcggtagc ctcactcacg
    13201 gcacgcaggt gaataaactg ctccagaaca tccgggagcg cgttaagacg accattttca
    13261 gccattaccc gaatcaggtt ctgaccgttt tcgtccagtt gctcaccaca aactgcgata
    13321 aacgactcgg cgagcgtttc tggcgcaagc gcgccagaga gaagctctgc catttgttcg
    13381 tttttggtta cctcggcggc aaacgccagc atgtcctgcc agcgttctac actttggtgt
    13441 tcgacggcaa agtcaaaagc tgctttggcg taggggcgag ctaccgtaat aaattcagac
    13501 atcagcccct ccctccttac agttcagcga caagtttatc cacgatgtcg ctgttagcag
    13561 cttcatccac ggaacgttcg atgatcttct cggcgccagc aacagccagg atagcaactt
    13621 gcttacgcag ctcttcacgg gcacgtttac gctcggcttc aatttccgcc tgcgcctggg
    13681 ccacgatttt agtacgttcc tgttctgcct cagctttcgc ttcgtccaga atctgcgagc
    13741 ggcgtttgtt agcctgctcg atgattacct gggcttccgc tttcgctttt ttcagctggt
    13801 cggtcgcgct ggcctttgca aggtcaaggt ccttatgtgc tcgttctgcg gaagcaaggc
    13861 cgtcagcaat ttctttttga cgtttttcga tggctgccat taatggcggc catacgtact
    13921 tcatgcagaa cagaacgaac aggacaaacg cgatggcctg gccgaggatt gttgcgttaa
    13981 gattcacagc acaatgcctc tatttagtta acgttctgat attgctcttt aaataaaagc
    14041 aacgcttact acgcgacagc gaacatcacg tacagaccca gacctacagc gatcatcggg
    14101 atagcatcca ccagacccat aacgataaag aactgagtac gcagcagagg aatcagatca
    14161 ggttgacgcg ctgcgccttc caggaattta cccccgagga tgccgatacc gatcgcagca
    14221 ccgattgccg ccagacccat catcacagcg gcagccatgt acagcagatc catattcagg
    14281 ttttccatga cagtctccag tttgtttcag ttaaaacgta gtagtgttgg taaattaatg
    14341 ttcttcagac gccatcgaca gatagacgat cgtcagaacc atgaagatga aggcttgcag
    14401 cgtaatgatc aggatgtgga aaatggccca cggcacattc aggatccact gtgaccacca
    14461 cggcaacaga ccagcaatca gaatgaaaat cagctcaccg gcatacatgt taccgaacag
    14521 tcgcaaaccg agtgaaactg gtttggacag caggcttacc ccttcaagga ttaagttgac
    14581 aggaatgaac gcccagtgat tgaacggctg cagcgtcaac tctttcgtga agccgccgat
    14641 gcctttcatt ttgatgctgt agaacagaat caggataaat acgcccagtg ccatagacag
    14701 cgttacgttc acgtcagcag acggaaccac acgcagtgca ggcagaccca gtacatgttc
    14761 agcaatgtac ggcagcaggt cgataggcag taaatccatc aggttcatca ggaataccca
    14821 gacgaagatc gtcagggcca gcggagcaat cagcttgctt ttgccatggt acatgtcttt
    14881 cacgctacca ttaacaaagc cgatcaccag ctcaatcgcg gtctgaaact tacctggcac
    14941 gccgctggtt gcctttttgg ctacactacg gaataaaacc aggaacaaca gacccagcac
    15001 caccgagaag aacatggagt caatattgat tgtccagaag gtggctgggg ggttatgtgg
    15061 atccaccagc gagaatgtac gcaggtccag ctgaaggtta ttcaggtggt gtcctatgta
    15121 atcctgcggc gtcatatttt ctgaagccat gatgcctttt accctttgtt gttaattaca
    15181 gccggtgcca gtatctgaac caccagcacc aaaacccacg taacgatcag cggcaagaat
    15241 accgccttta aaaccgccaa cgccaccacc agtaacacca acatcgccag aactttgaaa
    15301 gcttcgccaa atgcgaatgt ccaggccacc cggcctttcg ctggtgtatg cgcctggtga
    15361 cgccaggcaa atatcataaa caaaacgtta ggcagaaaga ctgccaggcc cccgcttatt
    15421 gcagagacgc cccagaaggg gtctttgagg ctgaacagca atccacttgc tatcaccacc
    15481 agtaactgaa cgagcagaag cttccgagca acgtttcgac tcacgagcga cacagacatc
    15541 acgtttttca ctcctgctcc cttcgaggta tgccgcgtgt cgtataaaac tttctttaag
    15601 gcttagagtc aagcatcaaa aagcggtcaa attatacggt gcgcccccgt gatttcaaac
    15661 aataagtagc caaaaggtga ataaatgttt aaatattttt ccagtgcata caattgcgac
    15721 ttttctgcaa accctgttcg atcatgaaaa actgtaaata acgcgtaaac actggtgata
    15781 aagcgtgctt cagatcacat attacgcatg ttcgcgcaca gcatatttat ttacttggca
    15841 aatgatgcct ttgcaagttt atgatatttc agtctaaaaa cagatactgt tttaataaat
    15901 gacattcaca taacaaaaac cacccattga catttttaat aatgttttaa cagtcaatga
    15961 tgattcttag cgccgatttt tagcagactg atattttcac ttatgactta ttttctgctt
    16021 accaaaaaaa gccacgttat cttgttgata caaaagagtg aacgtggcgt taaatgtaac
    16081 cagttatatc agtagaaaac ctggttgttg ttaacagtct aaccggtcaa ttttttatga
    16141 tttttttgat aaaaattaaa ttttatttgc tttaatcacc accagatgac gttcgccatc
    16201 cagggctgga acctgaagtt taaccactga ttcgacctga tattcttcgg gcaacaaagc
    16261 aatttcatct tccggcattt gccctttcag cgcgtagaaa cggccttgct caccaggaag
    16321 atggtggcac cagctcacca tatcgttcag agaggcaaaa gcgcggctaa ttacgccatc
    16381 aaatggcggc tctgacggaa actcttctac cctgctctgt actggctcaa tattctccag
    16441 tttaagctca tgttgcacct gacgaaggaa acgcacgcgt ttaccaaggc tatccaacaa
    16501 cgtgaaatgg gcttcaggac gcacgataga gagtggaatg ccaggcagtc ccggtccggt
    16561 gccgacatcg ataaaccgtt caccttgcag atacggtgcc accacaatgc tatcgagaat
    16621 atggcgtacc agcatctcat taggatcacg aaccgaagtc aggttgtacg ctttattcca
    16681 tttatgcagc atattcacgt aggcaataag ctggtttttc tggtgatcgg taagcgaaat
    16741 acctgcgtct ttcagcagta aggagagttt gttgagcacg gtgattacct gttcttgatg
    16801 cgttgcctgg taagcgggtg cttaccaggc atttttaatg tgttatgcgc tacgacgcag
    16861 cataccctgt tttttcagcc acaccagcag aatggagata gccgcaggcg tgacgccaga
    16921 aatacgcgaa gcctggccaa tagaggccgg tttgtgatcg ttaagtttgg cgatcacttc
    16981 gttagaaaga ccggatacct ggcggtaatc cagcgtcgct ggtagcaggg tattctcgtt
    17041 acgcagctgc ttttcgatct catcttgctg gcgcgcgata taaccttcgt atttaacctg
    17101 aatctcaacc tgttccgcag cctgttcatc tgtcaacgca ggggcaaacg gcgtcagcgt
    17161 ggttaatttt tcataagtca tttcaggacg acgcagcaga tcttcaccac tggcttcacg
    17221 ggaaagcggc gcagtcaggt gagcattcac ttcggctgca gcttccgccg acggggttac
    17281 ccaggtcgat ttcagacgct gacgctcacg ctcgatattc tcaagtttct cgttaaagcg
    17341 cgcccaacgt tcgtcatcca ccaggcccag ttcgcgaccg atttcagtca aacgcagatc
    17401 cgcattatct tcgcgcagca tcagacgata ttctgcgcgc gaggtaaaca tacgatacgg
    17461 ttctttggtt cctaaagtgc acaggtcatc aaccagtacg ccgagatatg cctgagaacg
    17521 cgccggagcc caaccttctt tgtcagcaga cagacgggca gcgttaagac cagccagcaa
    17581 accttgcgca gcggcttctt cgtaaccggt agtgccgtta atctgaccag caaagaacag
    17641 cccctggata aacttgctct ccagcgtcgg tttcaggtcg cgtggatcga agaagtcata
    17701 ctcaatggca taacccggac gcacgatctt cgcgttttcc atcccctgca tggagcggac
    17761 tatttgcatc tgcacatcga acggcaggct ggtggagata ccgttcggat aaatttcgtt
    17821 agaggtcagc ccttccggtt caaggaagat ctgatgctga tttctgtcgg caaagcgcat
    17881 gactttgtct tcgatcgacg ggcagtagcg tgggccgaca ccttcgatca cccctgcgta
    17941 cattgggcta cgatcgaggt tactgcggat cacatcatgg gttttctcgt tggtatgagt
    18001 gatataacac ggcacctgct ggggatgctg ggacgcattg cccataaacg agaataccgg
    18061 cattgggtta tcgccatgct gttgcgccag tacgctaaag tcgatggttc gagcatcaat
    18121 acgcggtggt gtcccggttt tcagacgacc aacgcgcagc ggcagttcac gcaaacggcg
    18181 agaaagcgga atggacggcg gatcaccagc acggccaccg ctgtaattat ccagaccgat
    18241 atgaatttta ccgtcgagga acgtcccaac ggtgagcacg acagctttgg cacggaactt
    18301 cagtcccatt tgggtaacgg caccgaccac gcgatctttt tcgacaataa gatcttcaac
    18361 tgcctgctgg aagatcatca ggttcggttg gttctccagc gccgtacgta ccgcctgacg
    18421 gtagagcaca cgatctgcct gagctcgggt agcgcgaacc gccggtccct tgcttgcgtt
    18481 tagtatccta aactggatac ccgcctgatc gatcgctttc gccatcagac cgccgagtgc
    18541 atccacttct tttaccagat gtcccttccc aataccgccg atcgccgggt tgcagctcat
    18601 ctgccccaga gtgtcgatat tgtgtgtcaa aagcagagtc tgttgaccta tacgcgccgc
    18661 ggccatcgcg gcctcggtgc ctgcatgacc cccgccaatg atgatgacgt caaaaggatc
    18721 cggataaaac atggtgattg cctcgcataa cgcggtatga aaatggattg aagcccgggc
    18781 cgtggattct actcaacttt gtcggcttga gaaagacctg ggatcctggg tattaaaaag
    18841 aagatctatt tatttagaga tctgttctat tgtgatctct tattaggatc gcactgccct
    18901 gtggataaca aggatccggc ttttaagatc aacaacctgg aaaggatcat taactgtgaa
    18961 tgatcggtga tcctagacag tataagctgg gatcagaatg aggggttata cacaactcaa
    19021 aaactgaaca accgttgttc tttggataac taccggttga tccaagcttc ctgacagagt
    19081 tatccacagt ggatcgcacg atctgtatac ttatttgagt aaattaatcc acgatcccag
    19141 ccattcttct gctggatctt ccggaatgtc gtgatcaaga atgttgatct tcagtgtttc
    19201 gcctgtctgt tttgcaccgg aatttttgag ttctgcctcg agtttatcga tagccccaca
    19261 aaaggtgtca tattcacgac taccaatacc tattgcgcca aagcggaccg cagaaagatc
    19321 gggcttctgt tcctgcaatg cttcatagaa aggagaaagg ttgtccggaa tatctccggc
    19381 accgtgggtt gagctgataa ccagccagat ccccgaggca gataaatctt ctaacagcgg
    19441 accgtgcagc gtttcggtgg taaaacccgc ctcttccagc ttttcagcca ggtgttctgc
    19501 tacatattcg gcaccgccga gggtgctgcc gctgataaga gtgatatctg ccataaaccg
    19561 ccacctttat taagagtggc gtattgtacg ctgtgaacgc gttgggatct acctgtggaa
    19621 aagtatggga ttaaaaaagc cgatcagggc ttaatggtac gcatgatcgg gttctgcagg
    19681 acgatcagtg tctcggtgga ctgaatttca tcaattgttt ggatcttgtt gataagtaca
    19741 tgctggagag catcgatcga acggcacatc acttttataa agatgctgta gtggccggtt
    19801 gtgtaatagg cttcagtgac ttcatcgagg ctttccagct ttgccagcgc ggaaggatag
    19861 tctttggcgc tctttaatat aatgccgata aagcagccta cgtcataacc aagctgcttc
    19921 gggctgacat caatacgcgc cccggtaatg atccccgcct gcttcatttt ctctactcga
    19981 acgtgaatcg tccccggact gacgccaaat tgtttcgcca gttcggcgta agcggtgcgc
    20041 gcattgccca ttaatgcttc caggatgcca cggtccagat tgtcgattag ataattttcc
    20101 ataggatttt cttatgcgga ttgatgattc attctatttt agccttcttt tttaatgaat
    20161 caaaagtacg cgaggctttt tattgaatga ttattgcatg tgtgtcggtt tttgttgctt
    20221 aatcataagc aacaggacgc aggagtataa aaaatgaaaa ccgcttacat tgccaaacaa
    20281 cgtcaaatta gcttcgtgaa atctcacttt tctcgtcaac tggaagaacg tctggggctg
    20341 atcgaagtcc aggcgccgat tcttagccgt gtgggagatg gcacgcagga taacttgtcg
    20401 ggctgtgaaa aagcggtgca ggtaaaagtg aaagctctgc ctgatgccca gttcgaagtg
    20461 gttcattcac tggcgaagtg gaaacgtcag accttagggc aacacgactt cagcgcgggc
    20521 gaagggctgt acacgcacat gaaagccctt cgccccgatg aagaccgtct ttccccgttg
    20581 cactcggtct atgtagacca gtgggactgg gaacgcgtaa tgggcgacgg cgagcgtcaa
    20641 ttctcgactc ttaaaagcac ggtagaggcg atctgggcgg gaattaaagc aaccgaagct
    20701 gcggttagcg aagagtttgg cctggcaccg ttcctgccgg atcagatcca tttcgtacac
    20761 agccaggagt tactgtctcg ttatccggat ctcgatgcca aagggcgtga gcgggcgatt
    20821 gcgaaagatc ttggcgcggt attcctcgtc gggattggcg gcaagttgag tgatggtcat
    20881 cgtcacgacg tgcgcgcacc ggattatgat gactggagca ccccgtcaga gctgggccat
    20941 gcgggtctga acggcgatat tctggtatgg aacccggtac tggaagatgc gtttgagctt
    21001 tcttccatgg ggatccgcgt ggatgccgac acgctgaagc atcagctggc gctgaccggt
    21061 gacgaagatc gcctggagct ggagtggcat caggcgctgc tgcgcggtga aatgccgcag
    21121 accatcggcg gcggtatcgg ccagtctcgt ttgaccatgc tgctgctgca actgccacat
    21181 atcggccagg ttcagtgtgg tgtatggccg gcagctgttc gcgagagcgt cccttctctg
    21241 ctgtaataat ttatcgccgc cagcgtctga gcaggcggct tcgcatcccg gtatcaaagc
    21301 gccagatatg atcgaaaatg cgcatgatgc cgggtttgcc gtgtgccgac atcgccacgg
    21361 catgaaagcg atgctgatgt atccgctgca actctttcac tttactcgtc acgtcgtcag
    21421 gcaaccgctg ggcgataaaa tcagaaatca ccaccgcatc ggcatcaaac cattccctgc
    21481 tttgcaagcg ttccataatg gcgcgaaaac aactggcaag atcggtgccg ccacgaaact
    21541 gctgacttaa aaagcggatc gcttgctcaa taccttgtgg ccctgaaagc tcataacgga
    21601 cgatctcggt ggaaaatagc ataatatagc agcgccggtt ttcagcgagg gctatgcgca
    21661 tcaaggccag gcagaacgct ttcgcacact gttcattaaa gccgcccatt gagccggaag
    21721 tatccacgca gacaataaac ggtccgcgcg gctgttcgtc gtaatcttta tgcaccaccg
    21781 ggcgttcgat cattttttca cgccacgact caccgtgcag gcgataggta agcaactgtt
    21841 tttccaccag ccgacggtaa aactcatact ccagttccgt tatccctagt gtcgccagtt
    21901 ctggcggcaa gagacgtaaa atatcatcgc tttgctgcag accatctacc tgctcaggaa
    21961 ctgtcgccgg ttcgcgcacc atggtgcgga aggtttccat ctgcgcatcg ttgcgcggta
    22021 ttgatttggc ttcccgggaa cgccccagct gttctgccag acgtttcagt tccggctgtt
    22081 cgttaagaaa ttcaccgtat ttcacaatca actgatagtc gccacgttta agctgaccgg
    22141 cactcatatc ccacagacga ccagctgcgg tattgttatc tgcgagaatc ggttcaagtt
    22201 gcccgctcag cgtcatgcgt tcctgaactt cactcaacag ttgttcgcgt tcttcttcta
    22261 atagctgctg atttaacgtc gttgcttgca cgatcaggct taaacgccag cgctggagaa
    22321 aaagcgtgtg taacgctgaa gtgatcgtac tgttagcatc aaccaactgt cgggcttgtt
    22381 ctgcccatgg ggaattcaga cgatgcagta agtctaggat ttgtggtagc tgcacaataa
    22441 actgcggcgt ggagaggagc tggctttgct gatagcacat cacctcttcg gtgagttccg
    22501 gcgggactcg ggcatctttc agccgactgc gcagcgcctc acgccagcgg ggaacatcat
    22561 cagtgattgc cgccttcagt cgtgggaatt tttcaaagaa gactgccagc tgcggtgagg
    22621 ccagcagcgc gatgatcatc tcttcgatca atccctcttc gctgacggcc agcatcacat
    22681 taagcgtatc cagcgttagc attgttgtgc ctggcgaatc tgtgcgccga catcctgtag
    22741 gctggcttca atgcgaccta accagtcgcc agggataaac agacattttt gctgttcgct
    22801 gaataaagcg tgttgcttgc gccagtcgct ttcaagctct tccagttgtt gttttatttc
    22861 gctgggcaga ccttcagccg atgaaccggg aagtgctagc gtactgcctt gtaaactcac
    22921 atcgcgtaca accagatgtt gggcgctatc gacttccaga ttcagtttct gggcaaagcc
    22981 gataccgttc agtttgccgc gaatctcacc acctttactc agccactgtt ccagcgcgct
    23041 acgctcaaag gagatatgaa ccacttccat atcatgcagt tttaacggtt tttgcagcag
    23101 cagagtcaga gtggaagcag taacgttaac agggagttga tactgctgac gacggctaaa
    23161 aatgccgccc agacgaatta ccgttaaggc tgttttatcg ctttgttgct gctgaagttg
    23221 caggtgacgt tgcacaatcg cgcccaggcg ggtcaacatc ccttgctgtt gccaggcgtg
    23281 accggtcatc aatacatcaa tttgttgttg tatcaaattc aggctttgcg cgtcatacca
    23341 caggcaatct ttcagcaaaa tgagatcaac cggggcgaca gcactgcgac cgctaaaaaa
    23401 ggcgctggcc tgcaataagc ggatcgcttt tttccagcga cgatccgaga cataaggcgc
    23461 atccggtaat ttatccagtt gctggcgcag cataaaaatc agctcaaata catgatcggg
    23521 cagcgtaatt tcaccaatct ctttctgcca gcgttcatat tcttcatctg tgatctgcaa
    23581 ggaggcagga accggattgt cattttcatc ctgttgactg gtcagcatgg agcggaaatt
    23641 cgctttatcc tgcactttat ctaaccacag acgaatcagc atgcggtcat ataacgcttc
    23701 cagactgctg tctgcttccg gcagctcgtt ggaggccgcc accagcagac gcatcgggat
    23761 tttttctacg agtgcgccgt tgcggaactg gcgctcgtta atggcggtga gcagggtatt
    23821 aagaattgcc ggacccgctt tccagatctc atccagaaag acgatttcag cttccggcag
    23881 gtaaccgctg gttaaacgtt catagcgccc ttcatctttt agcgcctgaa tagaaagggg
    23941 accaaaaact tcttccggcg tggagaagcg ggtcatcaga tattcaaacg cgcgggcatt
    24001 ctgaaaggcg aattttaagc gccgggcgat caaacttttg gcaatacctg gtgggccaag
    24061 gaggaacaca ctttcaccac ttaatgccgc taataaacac aagcggatgg cgtggctacg
    24121 ttcataaagc cccttttcca acgaactgct caggcgggaa attctttccg ctaataaatg
    24181 agggtgagcc ataatgaagt ggcgtccttt cgtcaaaagt tctgcgtaaa ttgcgagtat
    24241 agacgtttct tgctggtggc taaaatagtc tcaaaggagg ggtatttttc tttgagccag
    24301 gttaatgtgg ccgcatttag gagtacgatt ttgccgttaa tcgtgcatac tgtgcgcttt
    24361 tttgtgggcc aagggactaa gcacacattt catatttcaa cgaaagacta gtctatgagc
    24421 actgataata agcaatcatt gcccgcgatt accctcgcgg cgattggagt tgtctacggc
    24481 gatattggta ccagcccgtt atatacactt cgtgaatgtt tgtccggcca gtttggtttt
    24541 ggcgttgaac gcgatgccgt gtttggcttt ttatcgctga tcttctggct gctaatcttt
    24601 gtggtttcca ttaaatatct caccttcgtg atgcgggcag ataacgccgg tgaagggggg
    24661 atcctgacgt tgatgtcgct tgccgggcgt aatacgtcgg cgcgaaccac atcaatgctg
    24721 gtgattatgg ggctaatcgg cggcagcttt ttctatggtg aagtcgtcat tactcccgct
    24781 atttcggtga tgtccgccat tgaaggtctg gaaatcgtcg ccccgcagct ggatacctgg
    24841 atagttcccc tctcaattat cgttctcaca ttattattca tgattcaaaa acatggcacg
    24901 gctatggtcg gtaagctgtt tgcgccgatc atgctgacct ggtttttgat tctggcaggg
    24961 ctggggttac gtagcattat tgctaacccg gaagtgctgc atgcactgaa tccgatgtgg
    25021 gcggtgcatt tcttccttga atacaaaacg gtttctttta ttgcattagg ggcagtggtg
    25081 ctgtcgatta cgggggtcga ggcgctgtat gctgatatgg ggcactttgg taagttccct
    25141 attcgtctgg cgtggttcac cgtcgtattg ccttccttaa cccttaatta cttcggccag
    25201 ggagcgctgt tgttaaagaa tccggaagcg attaagaacc cgttcttcct gttggcaccg
    25261 gactgggcgc tgatcccgct gctgatcatc gccgcactgg cgacggtaat tgcctcgcag
    25321 gcggttatct ctggcgtctt ctcattgacg cgtcaggcgg tacgtctggg atatttgtcg
    25381 ccgatgcgca ttattcacac ttccgaaatg gagtcagggc aaatctatat tccctttgtg
    25441 aactggatgc tctatgtcgc ggtcgtgatt gtgattgtca gctttgagca ctccagcaac
    25501 ctggcggcgg cgtacgggat tgcggtgacc ggaaccatgg tgctgacgtc tattctctcg
    25561 actaccgtgg cacgtcagaa ctggcactgg aataagtatt ttgttgcgct gatcctgatt
    25621 gctttccttt gtgtcgatat tcccttgttc accgctaacc tcgataaact gctctccggc
    25681 ggctggttgc cattgagcct cggtacggtg atgtttatcg tgatgaccac ctggaagagc
    25741 gagcgtttcc gcttactgcg gcggatgcat gaacatggta actctctgga agcgatgatt
    25801 gcttcgctgg agaaatcacc gcccgttcgc gtgcctggga ccgcggtgta tatgtcgcgt
    25861 gcaatcaacg tcattccctt tgcgctgatg cataacctga aacataacaa ggtattgcat
    25921 gagcgggtga ttctgttaac tctgcgcacc gaagacgcgc catatgtcca taacgtccgt
    25981 cgggtacaga ttgaacaact gtcgcccact ttctggcgcg tggtggcaag ttatggttgg
    26041 cgagaaacgc caaacgtgga agaagttttc caccgctgcg gtctggaagg attaagttgc
    26101 cggatgatgg aaacctcctt ctttatgtcg catgagtcgt tgatcctcgg caaacgcccg
    26161 tggtatttgc gtctgcgcgg caagctgtac ttgctgctgc aacgtaatgc gctgcgggca
    26221 ccggatcaat ttgaaatccc gccaaacaga gttatcgaac tgggtactca ggtcgaaatc
    26281 taacgccaga cgcctccttt ctccataagg gggcgttttt gttttcatgg ttaatcacca
    26341 tgtaaaacgt ttcgaggttg atcacatttc cgtaacgtca cgatggtttt cccaactcag
    26401 tcaggattaa actgcgggtc agcgaaacgt ttcgctgatg gagaaaaaaa tgaaaaaagg
    26461 caccgttctt aattctgata tttcatcggt gatctcccta ctgggacata ccgatacgct
    26521 ggtggtgtgt gatgctggtt tacccatccc caaaagtaca acgcgtatcg atatggcatt
    26581 aacccagggt gtaccttctt ttatgcaggt gctgggcgtc gtcacaaatg aaatgcaggt
    26641 cgaggctgtc attatcgcgg aagagatcaa acaacataat ccgcaactcc acgaaacgtt
    26701 gctcactcac cttgagcagc tgcaacaaca tcagggaaac accattgaaa ttcgttacac
    26761 cacgcatgaa caattcaaac aacaaaccgc agaaagtcag gcggtaattc gcagcggaga
    26821 atgttctccg tatgcgaata tcattctctg tgctggcgtg acgttctgag gccgtcatgg
    26881 aagcattact tcagcttaaa ggcatcgata aagccttccc gggcgtaaaa gccctctcgg
    26941 gcgcagcgtt aaatgtctat ccgggccgcg tgatggcgct ggtgggcgaa aacggcgcgg
    27001 gtaaatccac catgatgaaa gtgcttactg gtatctatac tcgcgatgcc ggtacgcttt
    27061 tatggttggg gaaagaaacg acatttaccg ggccgaaatc ttcccaggaa gccgggattg
    27121 ggattatcca ccaggaactg aacctgatcc cgcagttaac cattgccgaa aacattttcc
    27181 tcggtcgtga gtttgttaat cgctttggca aaattgactg gaaaaccatg tatgccgaag
    27241 cggataaatt gctggctaaa cttaacctgc gctttaaaag cgacaaactg gtgggcgatc
    27301 tttccatcgg tgatcagcaa atggttgaaa tcgccaaagt gctgagcttt gagtcgaaag
    27361 tcatcattat ggatgaaccg accgatgcgc tgaccgatac cgaaaccgaa tccttgttcc
    27421 gcgtcatccg cgagctgaaa tcgcaaggcc gcggtattgt ctatatctcc caccgcatga
    27481 aagaaatctt cgagatttgc gatgacgtta ccgtttttcg tgacggacaa tttattgctg
    27541 agcgcgaagt ggcatcactg accgaagatt ctctgattga gatgatggtg ggccgcaagc
    27601 tggaagatca atatccgcac ctgaacaaag cgccgggaga tatccgcctg aaagtcgata
    27661 atctctgcgg acctggcgtt aacgatgtct cttttacttt acgcaaaggc gaaattcttg
    27721 gcgtctctgg tttgatgggc gctgggcgta ccgaactgat gaaagtgctc tacggtgcac
    27781 tgccgcgcac aagcggttac gtcaccctgg atgggcatga agtcgttacc cgttcgccgc
    27841 aggatggcct ggcaaacggc attgtttata tctccgaaga ccgtaaacgt gacggtttag
    27901 tgctgggcat gtcagtaaaa gagaacatgt cgctgaccgc gttgcgctac ttcagccgcg
    27961 ctggcggcag tttgaagcat gccgatgaac agcaggctgt gagtgatttc attcgtctgt
    28021 ttaatgtgaa aacgccatcg atggaacagg caattggtct gctttccggt ggcaatcagc
    28081 aaaaagtagc gattgcccgt ggtctgatga cacgccctaa agtgttgatc ctcgatgagc
    28141 ctacccgtgg cgtagatgtc ggcgcgaaaa aagagatcta tcaactgatt aaccagttca
    28201 aagccgatgg cttgagcatc attctggtgt catcggagat gccagaagta ttaggcatga
    28261 gcgatcgcat catcgtcatg catgaagggc atctcagcgg ggaatttact cgtgagcagg
    28321 ccacccagga agtgttaatg gctgccgctg tgggcaagct taatcgcgtg aatcaggagt
    28381 aaaaaaatga caacccagac tgtctctggt cgccgttatt tcacgaaagc gtggctgatg
    28441 gagcagaaat cgcttatcgc tctgctggtg ctgatcgcga ttgtctcgac gttaagcccg
    28501 aactttttca ccatcaataa cttattcaat attcttcagc aaacctcggt gaacgccatt
    28561 atggcggtcg ggatgacgct ggtgatcctg acgtcgggta tcgacttgtc ggtaggttct
    28621 ctgttggcgc tgaccggcgc agttgctgca tctatcgtcg gcattgaagt caatgcgctg
    28681 gtggctgtcg ctgccgctct cgcgttaggt gccgcaattg gtgcggtaac tggggtgatt
    28741 gtagcgaaag gtcgcgtcca ggcgtttatc gctacgctgg ttatgatgct tttactgcgc
    28801 ggcgtgacca tggtttatac caatggtagc ccagtgaata ccggctttac tgagaacgcc
    28861 gatctgtttg gctggtttgg tattggtcgt ccgctgggcg taccgacgcc agtctggatc
    28921 atggggattg tcttcctcgc ggcctggtac atgctgcatc acacgcgtct ggggcgttac
    28981 atctacgcgc tgggcggcaa cgaagcggca acgcgtcttt ctggtatcaa cgtcaataaa
    29041 atcaaaatca tcgtctattc tctttgtggt ctgctggcat cgctggccgg gatcattgaa
    29101 gtggcgcgtc tctcctccgc acaacccact gcggggactg gctatgagct ggatgctatt
    29161 gctgcggtgg ttctgggcgg tacgagtctg gcgggcggaa aaggtcgcat tgttgggacg
    29221 ttgatcggcg cattaattct tggcttcctt aataatggat tgaatttgtt aagtgtttcc
    29281 tcctattacc agatgatcgt caaagcggtg gtgattttgc tggcggtgct ggtagacaac
    29341 aaaaagcagt aataacgact acaggacatc ttgaatatga acatgaaaaa actggctacc
    29401 ctggtttccg ctgttgcgct aagcgccacc gtcagtgcga atgcgatggc aaaagacacc
    29461 atcgcgctgg tggtctccac gcttaacaac ccgttctttg tatcgctgaa agatggcgcg
    29521 cagaaagagg cggataaact tggctataac ctggtggtgc tggactccca gaacaacccg
    29581 gcgaaagagc tggcgaacgt gcaggactta accgtccgcg gcaccaaaat tctgctgatt
    29641 aacccgaccg attccgacgc agtaggtaat gctgtgaaga tggctaacca ggcgaacatc
    29701 ccggttatca ctcttgaccg ccaggcaacg aaaggtgaag tggtgagcca cattgcatct
    29761 gataacgtac tgggcggcaa aatcgccggt gattacatcg cgaagaaagc gggtgaaggt
    29821 gccaaagtta tcgagctgca aggcattgcc ggtacatccg cagcccgtga acgtggcgaa
    29881 ggtttccagc aggccgttgc tgctcacaag tttaatgttc ttgccagcca gcctgcagat
    29941 tttgatcgca ccaaaggttt gaacgtaatg cagaacctgt tgaccgctca tccggatgtt
    30001 caggctgtat tcgcgcagaa tgatgaaatg gcgcttggcg cgctgcgcgc actgcaaact
    30061 gccggtaaat cggatgtgat ggtcgtcgga tttgacggta caccggatgg cgaaaaagcg
    30121 gtgaatgatg gcaaactagc agcgactatc gctcagctac ccgatcagat tggcgcgaaa
    30181 ggcgtcgaaa ccgcagataa agtgctgaaa ggcgagaaag ttcaggctaa gtatccggtt
    30241 gatctgaaac tggttgttaa gcagtagttt taatcaggtt gtatgacctg atggtgacat
    30301 aaatacgtca tcgacagatg aacgtgtaat ataaagaaaa gcagggcacg cgccacccta
    30361 acacggtggc gcattttatg gacatcccga atatgcaaaa cgcaggcagc ctcgttgttc
    30421 ttggcagcat taatgctgac cacattctta atcttcaatc ttttcctact ccaggcgaaa
    30481 ccgtaaccgg taaccactat caggttgcat ttggcggcaa aggcgcgaat caggcggtgg
    30541 ctgctgggcg tagcggtgcg aatatcgcgt ttattgcctg cactggcgat gacagcattg
    30601 gtgagagcgt tcgccagcag ctcgccactg ataacatcga tatttctccg gtcagcgtga
    30661 tcaaaggcga atcaacaggt gtggcgctga tttttgttaa tggcgaaggt gagaatgtca
    30721 tcggtattca tgccggcgct aatgctgccc tttccccggc gctggtggaa gcgcaacgtg
    30781 agcgtattgc caacgcgtca gcattattaa tgcagctgga atcaccactc gaaagtgtga
    30841 tggcagcggc gaaaatcgcc catcaaaata agactatcgt tgcgcttaac ccggctccgg
    30901 ctcgcgaact tcctgacgaa ctgctggcgc tggtggacat tattacgcca aacgaaacgg
    30961 aagcagaaaa gctcaccggt attcgtgttg aaaatgatga agatgcagcg aaggcggcgc
    31021 aggtactgca tgaaaaaggt atccgtactg tactgattac tttaggaagt cgtggtgtat
    31081 gggctagcgt gaatggtgaa ggtcagcgcg ttcctggatt ccgggtgcag gctgtcgata
    31141 ctattgctgc cggagatacc tttaacggcg cgttaatcac ggcgttgctg gaagaaaaac
    31201 cattgccaga ggcgattcgt tttgcccatg cagccgctgc gattgccgta acacgtaaag
    31261 gcgcacaacc ttccgtaccg tggcgtgaag agatcgacgc atttttagac aggcagaggt
    31321 gacgcttggc tacaatgaaa gatgttgccc gcctggcggg cgtttctacc tcaacagttt
    31381 ctcacgttat caataaagat cgcttcgtca gtgaagcgat tacagccaaa gttgaagcgg
    31441 cgattaaaga actcaattac gcgccatcag ccctggcgcg tagcctcaaa ctcaatcaaa
    31501 cacataccat tggcatgttg atcactgcca gtaccaatcc tttctattca gaactggtgc
    31561 gtggcgttga acgcagctgc ttcgaacgcg gttatagtct cgtcctttgc aataccgaag
    31621 gcgatgaaca gcggatgaat cgcaatctgg aaacgctgat gcaaaaacgc gttgatggct
    31681 tgctgttact gtgcaccgaa acgcatcagc cttcgcgtga aatcatgcaa cgttatccga
    31741 cagtgcctac cgtgatgatg gactgggctc cgttcgatgg cgacagcgat cttattcagg
    31801 ataactcttt gctgggcgga gacttagcaa cgcaatatct gatcgataaa ggtcataccc
    31861 gtattgcctg tattaccggt ccgctggata aaactccggc gcgcctgcgg ttggaaggtt
    31921 atcgggcggc gatgaaacgt gcaggcctca acattcctga tggctatgaa gtcactggtg
    31981 attttgaatt taacggcggg tttgacgcca tgcgccaact gttatcacat ccgctgcgtc
    32041 ctcaggctgt ctttaccgga aatgacgcta tggctgttgg cgtttaccag gcgttatatc
    32101 aggcagagtt acaggttccg caggatatcg cagtgattgg ctatgacgat atcgaactgg
    32161 caagctttat gacgccacca ttaacaacta tccaccaacc gaaagatgaa ctgggggagc
    32221 tggcgattga tgtactcatc catcggataa cccagccggc cctacagcaa caacgattac
    32281 aacttactcc gattctgatg gaacgtggtt cggcttagat ttacgctgtc ttttgatcaa
    32341 attattacca tcggttgttt tcagtagcat gaacattgct gctgaagcaa cagtaataat
    32401 gcccatcgtg ataaacgtat agtggaattg ttcgactgtc gttgtgcctt ccatcccttc
    32461 gtaaacgcga aggacggccg cacttacagc aacgcctaaa ctgatagaca gttgctgcgt
    32521 gaccgccaga acactgttac cgctgctggc gttgtcatcg gtcagatcgg caagtgtgat
    32581 ggtattcatc gcggtaaatt gcgtcgacat agccatccct aatataaaca acggcaagat
    32641 cagcatccat atcgccattg ctggtgattg caaagagaac tgagcgatca ttagcccaat
    32701 aatcaccgtg atccccacta acgtatggcg atagcccaga cgacgtaaga cttgggtaac
    32761 catcgatttt gcaataatgg aacctaacgc tgtcggtgcc atcatacagc cagcaataaa
    32821 cgcctgatac ccaaatccta cctgtaacat caatggcata aggaacggta cacagccggt
    32881 ccccagacgg gttgcaatat tgcctacgat accgatcgag aaagtgcggg ttttaaataa
    32941 atctaatgaa attaatgggt ttggcgtgcg tcgcgcatgg agaatataga gaagcagtaa
    33001 ccagatgctg gtgacaatta ccgtcaaggc aatccagctg gcgacaatct tttccccgaa
    33061 tagctctatt ccgcttgaga agagaacaag gctgaggcca aacagcaaaa agccagtggt
    33121 atcgaatctg cgtcgtgcgg tggtgaaatt gggcatatgt ttgcgcgcgt aaagaaggcc
    33181 cgcaatacct atggggatat tgattaaaaa tatccagtgc caggttgccc aggtgaccag
    33241 cacgccgcca agaacggggc ctaaaattgg ccccaccaga cccggcatgg cgacaaaatt
    33301 caatacagga agaagttcat tacgaggata agcgcgcagt aaggccagcc gagcaacagg
    33361 catcatcatt gcgccgccta tcccctgaat aacccggaag acaaccagct gtggtagcga
    33421 gttggaaagt gcgcaggcca gagaacccaa cgtgaacaga ctcacggcaa gggtaaaaat
    33481 gcgacgtgta ccgaagcgat cggctagcca tccgcttacc ggaataagca tcgccaccgt
    33541 cagcgtataa ctgatgatgg ctgattgcat cgcgagagga gaacgattaa ggctatgagc
    33601 gattgcgggt aaggcggtat tcagaatagt ggcatcaagt gcctgcatga agaaggccat
    33661 cgccgcgatc cacggcaaac ccgccatact gcgcttcttt ttatcgctca ttcaatgtcc
    33721 tgttatcggg ttatcactta tctggtgagc gtagcagcgc ctgacaagct ttaaatgccg
    33781 cgtcgccatc gctttggata atcgcatcga caatcgcctg gtgcagatcc agctttatca
    33841 ctgtgtcgct ggtaattgac gtgaagtaag tgtgataaac cgaatggaat agcgaggcga
    33901 atgaggtcaa aaacggattg gcgctcattt catagatatg ctcatgccag gccatatcga
    33961 cttcgatcca gcgttcacgg cgaaagttct cttttaatgc cgccatttcg gccattaacg
    34021 tattgagatg cgccttctgt tccgcggtgc caacctttgc tgccagtagg caagcttgcg
    34081 gctccaaaca gatacgcata accagaaagt gatcgatgac ctgatgaaag ttctcttctg
    34141 tcatccacca ggtaagcaat tcctgatcaa gaaaattcca gttcgattgt ggcatgactc
    34201 gggtaccaat tcgcggtcgc ggtaaaacca tcccttttgc cgttaacgtt ttgaccgctt
    34261 cgcgtaccgc tgtacgactc actccaaatt gctcgcccag ctcaatctca ccaggcaaaa
    34321 tggtgccggg ttcatattca ccttttaaga tccgctgcgc cagcttctca gccagaacat
    34381 acgaaaggtt tttctgtgca gctaactgtt gtgcgcttaa aggcattact aatcttcctt
    34441 tttcttttta ttccttctta gtatgccacc aggaagtgtg attacggttg caaaaacggc
    34501 aaattgcttg ttttatggca cattaacggg gcttttgctg aaaaa
//
LOCUS       DADSYM010000034        34093 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2415
            SAMN08039579-rid11723173.denovo.034, whole genome shotgun sequence.
ACCESSION   DADSYM010000034 DADSYM010000000
VERSION     DADSYM010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039579
            Sequence Read Archive: SRR6362533
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 34093)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34093)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980162.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 03:39:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,484
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 4,347
            CDSs (with protein)               :: 4,347
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 43 of 85
            Pseudo Genes (incomplete)         :: 37 of 85
            Pseudo Genes (internal stop)      :: 19 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34093
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039579-rid11723173.denovo.034"
                     /strain="LGE2415"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8302 N 95.9628 W"
                     /collection_date="2015-08-24"
     gene            9..728
                     /locus_tag="J1Y83_001377"
     CDS             9..728
                     /locus_tag="J1Y83_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000225871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HBA7629538.1"
                     /translation="MVAKINRLLRLGPVLLLCTSFFAQSNMTVYPMAVSINSQGEGSV
                     RVISKSNEVQYIKATVFRIDNPSTPLENEVEIKSGDANHLVVMPPKFALPAGSSKTVR
                     FVAMEPEQQEKNYRVKFEAVPSIDDVATDKKDLSMQLTVNLIWGIVVSVPPQQPVTKL
                     EINSAKKLLNAGNQRLKVLTIAYCKNNSKDNSCKTQTVNKNIFPGQEKSLESISGYDK
                     IVVKYNNWITKDNGEFELAVH"
     gene            758..1258
                     /locus_tag="J1Y83_001378"
     CDS             758..1258
                     /locus_tag="J1Y83_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000755097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HBA7629539.1"
                     /translation="MKKVFVKSLLVAAMFSVAGSALAVQKDITVTANVDAALDMTQTD
                     NSALPKAVEMQYLPGQGLQSYQLMTKIWSNDVTKDVKMQLVSPAQLVQSLDASKIVPL
                     TVSWGDEEIKADAATTFSAVKIFASDAITNGSLAKNLMFAQTTKGVLETGIYRGVVSI
                     YLSQDI"
     gene            1351..4035
                     /locus_tag="J1Y83_001379"
     CDS             1351..4035
                     /locus_tag="J1Y83_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001666746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HBA7629540.1"
                     /translation="MDKKLLALLILASLSPAKATLTKIPAGFEVIAQGQQEYIEVYFA
                     GKSLGKYYAMVNLDTVTFLDSSSLYSKLELSTDDQKITHTVKEKLSQPLARHGELACG
                     FVRTDSGCGFLNTETVEIIYNDEESSVTLFLNPQWSSAFNSKSLYLNPDKNTVNAFIH
                     QQDINVLVQDDYQSLSIQGNGALGITENSYIGAHWNFNGYDADDVSDNNVDVSDLYYR
                     YDFLRRYYVQAGRMDNRTLFNAQGGNYTFNFLPLGAIDGMRIGSTLSYLNQTQSQQGT
                     PVMILLSRNSRVDAYRNEQLLGSFYLNSGSQFIDTSSFPPGSYSVALKVYENNQLTRT
                     ELVPFTKTGGLTDGNAQWFLQAGKTTSQVSDDESSAYQLGVRLPLHPQYELYAGLANA
                     DDVSAFELGNNWTADLGEAGNLAISASVFRNDDGGKGDMQQANWSHPGWPTLGFYRTN
                     SDGDACTTDNRESYNALSCYESISATVSQNLVGWNMMLGYTRTQNNTDDSLRWDKQQS
                     FENNYLRQTTAQSISETVQLSASRAFVMRDWILSTSLGFFHRNDNGSGSDDNGLYLSF
                     SLSDTPTMDSNNNSHSTNVSTDYRYSDQDGDQTSWQLSHTFYNDSFSHKELGVTVGGL
                     NTDTINSAVNGRWDGQYGNVYATVSDSYDRQNHDHLSAFTGTYSSTLAVSRYGINVGA
                     SGTDDLLGAVLVDVKGFSEQDEQSQDLQLEARVAGSRTLQLGQRDSVLFPYPGFQSGF
                     VEVNDSNQGNQQGTTNIINGAGNRELMLLPGKLRYREVSASFNYNYIGRLLLPASVEK
                     FPLVGLNSAMLLVAEDGGFTLEINGSEKELYLLSGQQFLKCPLSVLKKRASIRYSGDV
                     NCSVVSYSQLPESIQVQAQLKQPKLRGNVQTAQREIAP"
     gene            4032..5114
                     /locus_tag="J1Y83_001380"
     CDS             4032..5114
                     /locus_tag="J1Y83_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001352530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein CblD"
                     /protein_id="HBA7629541.1"
                     /translation="MINRLIAVVLCLFGMVAWVQATPNVTAEITYDLAVGRADYYFWN
                     EEESAGNNAYMWYECSYPNLQQTCTNSGNISTVQIYLTEQRSGMRWPLKLKGFKEAHV
                     MNQVPGCEGYVAKTHLRESNRYKCMATGDSTIYYADKKFLTLYLEQAEMKKLPIGGVW
                     KGQVKLHSNSPAQDYFADITLNTLDPNHIDVFFPEFAHATPRVQLDLHPTGSVNASNY
                     AQDLTTLDMCLYDGFNGNAISYEIMLKDEGLPAAGRRDGYFSIYRQGRTTTDDVERID
                     YRVKMYNPETGGQIDVRNNENMVWNSINLKRVRPVVLPGIRYAVMCVPTPLTLAVDKF
                     SVMDKQAGYYMGKLSVIFTPSLPTIN"
     gene            complement(5213..6511)
                     /locus_tag="J1Y83_001381"
     CDS             complement(5213..6511)
                     /locus_tag="J1Y83_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708898.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBA7629542.1"
                     /translation="MRKIKGLRWYMIALVTLGTVLGYLTRNTVAAAAPTLMEELNIST
                     QQYSYIIAAYSAAYTVMQPVAGYVLDVLGTKIGYAMFAVLWAVFCGATALAGSWGGLA
                     VARGAVGAAEAAMIPAGLKASSEWFPAKERSIAVGYFNVGSSIGAMIAPPLVVWAIVM
                     HSWQMAFIISGALSFIWAMAWLIFYKHPRDQKHLTDEERDYIINGQEAQHQVDTAKKM
                     SVGQILRNRQFWGIALPRFLAEPAWGTFNAWIPLFMFKVYGFNLKEIAMFAWMPMLFA
                     DLGCILGGYLPPLFQRWFGVNLIVSRKMVVTLGAVLMIGPGMIGLFTNPYVAIMLLCI
                     GGFAHQALSGALITLSSDVFGRNEVATANGLTGMSAWLASTLFALVVGALADTIGFSP
                     LFAVLAVFDLLGALVIWTVLQNKPAIEVAQESHNDPAPQH"
     gene            6994..8406
                     /gene="uxaC"
                     /locus_tag="J1Y83_001382"
     CDS             6994..8406
                     /gene="uxaC"
                     /locus_tag="J1Y83_001382"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019077755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HBA7629543.1"
                     /translation="MTPFMTEDFLLDTEFARRLYHDYAKDQPIFDYHCHLPPQQIAED
                     YRFKNLYDIWLKGDHYKWRAMRTNGVAERLCTGDASDREKFDAWAATVPHTIGNPLYH
                     WTHLELRRPFGITGKLLSPSTADEIWNECNELLAQDNFSARGIMQQMNVKMVGTTDDP
                     IDSLEHHAEIAKDGSFTIKVLPSWRPDKAFNIEQATFNDYMAKLGEVSDTDIRRFADL
                     QTALTKRLDHFAAHGCKVSDHALDVVMFAEANEAELDSILARRLAGETLSEHEVAQFK
                     TAVLVFLGAEYARRGWVQQYHIGALRNNNLRQFKLLGPDVGFDSINDRPMAEELSKLL
                     SKQNEENLLPKTILYCLNPRDNEVLGTMIGNFQGEGMPGKMQFGSGWWFNDQKDGMER
                     QMTQLAQLGLLSRFVGMLTDSRSFLSYTRHEYFRRILCQMIGRWVEAGEAPADINLLG
                     EMVKNICFNNARDYFAIELN"
     gene            8421..9908
                     /locus_tag="J1Y83_001383"
     CDS             8421..9908
                     /locus_tag="J1Y83_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="altronate dehydratase"
                     /protein_id="HBA7629544.1"
                     /translation="MQYIKIHALDNVAVALADLAEGTEVSVDNQTVTLRQDVARGHKF
                     ALTDIAKGANVIKYGLPIGYALADIAAGEHVHAHNTRTNLSDLDQYRYQPDFQDLPAQ
                     AADREVQIYRRANGDVGVRNELWILPTVGCVNGIARQIQNRFLKETNNAEGTDGVFLF
                     SHTYGCSQLGDDHINTRTMLQNMVRHPNAGAVLVIGLGCENNQVAAFRETLGDIDPER
                     VHFMICQQQDDEIEAGIAHLHQLYSVMRNDKREPGKLSELKFGLECGGSDGLSGITAN
                     PMLGRFSDYVIANGGTTVLTEVPEMFGAEQLLMDHCRDEATFEKLVTMVNDFKQYFIA
                     HDQPIYENPSPGNKAGGITTLEDKSLGCTQKAGSSVVVDVLRYGERLKTPGLNLLSAP
                     GNDAVATSALAGAGCHMVLFSTGRGTPYGGFVPTVKIATNSELAAKKKHWIDFDAGQL
                     IHGKAMPQLLEEFIDTIVEFANGKQTCNERNDFRELAIFKSGVTL"
     gene            9991..10542
                     /locus_tag="J1Y83_001384"
     CDS             9991..10542
                     /locus_tag="J1Y83_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgjV family protein"
                     /protein_id="HBA7629545.1"
                     /translation="MTAYWLAQGVGVIAFLIGITTFFNRDERRFKKQLSVYSAVIGVH
                     FFLLGTYPAGASAILNAIRTLITLRTRSLWVMAIFIVLTGGIGLAKFHHPVELLPVIG
                     TIVSTWALFRCKGLTMRCVMWFSTCCWVIHNFWAGSIGGTMIEGSFLLMNGLNIIRFW
                     RMQRRGIDPFKVEKTPSAVDERG"
     gene            complement(10546..11790)
                     /gene="sstT"
                     /locus_tag="J1Y83_001385"
     CDS             complement(10546..11790)
                     /gene="sstT"
                     /locus_tag="J1Y83_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HBA7629546.1"
                     /translation="MTTQRSPGLFRRLAHGSLVKQILAGLILGILLAWISKPAAEAVG
                     LLGTLFVGALKAVAPILVLMLVMASIANHQHGQKTNIRPILFLYLLGTFSAALAAVIF
                     SFAFPSTLHLSSSAGDISPPSGIVEVMRGLVMSMVSNPIDALLKGNYIGILVWAIGLG
                     FALRHGNETTKNLVNDMSNAVTFMVKLVIRFAPIGIFGLVSSTLATTGFSTLWGYAQL
                     LVVLVGCMLLVALVVNPLLVWWKIRRNPFPLVLLCLRESGVYAFFTRSSAANIPVNMA
                     LCEKLNLDRDTYSVSIPLGATINMAGAAITITVLTLAAVNTLGIPVDLPTALLLSVVA
                     SLCACGASGVAGGSLLLIPLACNMFGISNDIAMQVVAVGFIIGVLQDSCETALNSSTD
                     VLFTAAACQAEDDRLANSALRN"
     gene            complement(12115..13080)
                     /gene="alx"
                     /locus_tag="J1Y83_001386"
     CDS             complement(12115..13080)
                     /gene="alx"
                     /locus_tag="J1Y83_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001098790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family membrane protein Alx"
                     /protein_id="HBA7629547.1"
                     /translation="MNTVGTPLLWGGFAVVVTIMLAIDLLLQGRRGAHAMTMKQAAAW
                     SLVWVTLSLLFNAAFWWYLVQTEGRAVADPQALAFLTGYLIEKSLAVDNVFVWLMLFS
                     YFSVPAALQRRVLVYGVLGAIVLRTIMIFTGSWLISQFDWILYIFGAFLLFTGVKMAL
                     AHEDESGIGDKPLVRWLRGHLRMTDTIDNEHFFVRKNGLLYATPLMLVLILVELSDVI
                     FAVDSIPAIFAVTTDPFIVLTSNLFAILGLRAMYFLLAGVAERFSMLKYGLAVILVFI
                     GIKMLIVDFYHIPIAVSLGVVFGILVMTFIINAWVNYRHDKQRVE"
     gene            complement(13363..14349)
                     /locus_tag="J1Y83_001387"
     CDS             complement(13363..14349)
                     /locus_tag="J1Y83_001387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417558.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HBA7629548.1"
                     /translation="MIRFAVIGTNWITRQFVEAAHESGKYKLTAVYSRSLEQAQHFAN
                     DFSVEHLFTSLEAMAESDAIDAVYIASPNSLHFSQTQLFLSHKIHVICEKPLASNLAE
                     VDAAIACARENQVVLFEAFKTAYLPNFHLLRQALPKVGKLRKVFFNYCQYSSRYQRYL
                     DGENPNTFNPAFSNGSIMDIGFYCLASAVALFGEPKSVQATASLLASGVDAHGVVVMD
                     YGDFSVTLQHSKVSDSVLASEIQGEAGALVIEKLSECQKVCFVPRGSQMQDLTQPQHI
                     NTMLYEAELFATLVDEHLVNHPGLAVSRITAKLLTEIRRQTGVIFPADNVKL"
     gene            complement(14428..15120)
                     /locus_tag="J1Y83_001388"
     CDS             complement(14428..15120)
                     /locus_tag="J1Y83_001388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin high temperature exclusion protein"
                     /protein_id="HBA7629549.1"
                     /translation="MLRVFAHLLRRVCFSRRTLKIGCLLLLIAGATLFIADRVMVNAS
                     QQLTWSDVNAVPARNVGLLLGARPGNRYFTRRIDTAAELYHAGKVKWLLVSGDNGRKN
                     YDEASGMQQALIAKGVPAKVIFCDYAGFSTLDSVVRAKKVFGENHITIISQEFHNQRA
                     IWLAKQYGIDAIGFNAPDLNMKHGFYTQLREKLARVSAVIDAKILHRQPKYLGPLVMI
                     GPFSEHGCPAKE"
     gene            complement(15197..15560)
                     /locus_tag="J1Y83_001389"
                     /pseudo
     CDS             complement(15197..15560)
                     /locus_tag="J1Y83_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311994.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
     gene            15645..16781
                     /gene="rlmG"
                     /locus_tag="J1Y83_001390"
     CDS             15645..16781
                     /gene="rlmG"
                     /locus_tag="J1Y83_001390"
                     /EC_number="2.1.1.174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311993.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (guanine(1835)-N(2))-methyltransferase
                     RlmG"
                     /protein_id="HBA7629550.1"
                     /translation="MSHLDNGFRSLTLQRFPATDDVNPLQAWEAADEYLLQQLDDTEI
                     RGPVLILNDAFGALSCALAEHKPYSIGDSYISELATRENLRLNGIDESSVKFLDSTAD
                     YPQQPGVVLIKVPKTLALLEQQLRALRKVVTPDTRIIAGAKARDIHTSTLELFEKVLG
                     PTTTTLAWKKARLINCTFNEPPLADAPQTVSWKLEGTDWTIHNHANVFSRTGLDIGAR
                     FFMQHLPENLEGEIVDLGCGNGVIGLTLLDKNPQAKVVFVDESPMAVASSRLNVETNM
                     PEALDRCEFMINNALSGVEPFRFNAVLCNPPFHQQHALTDNVAWEMFHHARRCLKING
                     ELYIVANRHLDYFHKLKKIFGNCTTIATNNKFVVLKAVKLGRRR"
     gene            16972..17055
                     /locus_tag="J1Y83_001391"
                     /pseudo
     CDS             16972..17055
                     /locus_tag="J1Y83_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311992.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HigB family
                     toxin"
     gene            17052..17468
                     /gene="higA"
                     /locus_tag="J1Y83_001392"
     CDS             17052..17468
                     /gene="higA"
                     /locus_tag="J1Y83_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin HigA"
                     /protein_id="HBA7629551.1"
                     /translation="MIAIADILQAGEKLTAVAPFLAGIQNEEQYTQALELVDHLLLND
                     PENPLLDLACAKITAWEESAPEFAEFNAMAQAMPGGIAVIRTLMDQYGLTLSDLPEIG
                     SKSMVSRVLSGKRILTLEHAKKLAARFGISPALFID"
     gene            complement(17513..19531)
                     /locus_tag="J1Y83_001393"
     CDS             complement(17513..19531)
                     /locus_tag="J1Y83_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 2,4-dienoyl-CoA reductase"
                     /protein_id="HBA7629552.1"
                     /translation="MSYPSLFAPLDLGFTTLKNRVLMGSMHTGLEEYPNGAERLAAFY
                     AERARHGVALIVSGGIAPDLTGVGMEGGAMLNDANQIPHHRTITEAVHQEGGKIALQI
                     LHTGRYSYQPHLVAPSALQAPINRFVPHELTHEEILQLIDDFAHCAQLAREARYDGVE
                     VMGSEGYLINEFLTLRTNQRSDQWGGDYRNRMRFAVEVVRAVRERVGNDFIIIYRLSM
                     LDLVEGGGTFAEMVELAQAIEAAGATIINTGIGWHEARIPTIATPVPRGAFSWVTRKL
                     KGHVSLPLVTTNRINDPQVADDILSRGDADMVSMARPFLADAELLSKAQSGRADEINT
                     CIGCNQACLDQIFVGKVTSCLVNPRACHETKMPILPAVQKKNLAVVGAGPAGLAFAIN
                     AAARGHQVTLFDAHSEIGGQFNIAKQIPGKEEFYETLRYYRRMIEVTGVTLKLNHTVT
                     ADQLQAFDETILASGIVPRVPPIDGIDHPKVLSYLDVLRDKAPVGNKVAIIGCGGIGF
                     DTAMYLSQPGESTSQNIAGFCNEWGIDSSLQQAGGLSPQGMQIPRSPRQIVMLQRKAS
                     KPGQGLGKTTGWIHRTTLLSRGVKMIPGVSYQKIDDEGLHVVINGEPQILAVDHVVIC
                     AGQEPNRTLAQPLIDSGKTVHLIGGCDVAMELDARRAIAQGTRLALEI"
     gene            complement(19757..22108)
                     /gene="ygjK"
                     /locus_tag="J1Y83_001394"
     CDS             complement(19757..22108)
                     /gene="ygjK"
                     /locus_tag="J1Y83_001394"
                     /EC_number="3.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HBA7629553.1"
                     /translation="MKIKTILTPVACALLMSFSAHAANADNYKNVINRTGAPQYMKDY
                     DYDDHQRFNPFFDLGAWHGHLLPDGPNTMGGFPGVALLTEEYINFMASNFDRLTVWQD
                     GKKVDFTLEAYSIPGALVQKLTAKDVQVEMTLRFATPRTSLLETKITSDKPLDLVWDG
                     ELLEKLEAKEGKPLSDKTIAGEYPDYQRKISATRDGLKVTFGKVRATWDLLTSGESEY
                     QVHKSLPVQTEINGNRFTSKAHINGSTTLYTTYSHLLTAQEVSKEQMQIRDILARPAF
                     YLTASQQRWEEYLKKGLTNPDATPEQTRVAVKAIETLNGNWRSPGGAVKYNTVTPSVT
                     GRWFSGNQTWPWDTWKQAFAMAHFNPDIAKENIRAVFSWQIQPGDSVRPQDVGFVPDL
                     IAWNLSTERGGDGGNWNERNTKPSLAAWSVMEVYNVTQDKTWLAEMYPKLVAYHDWWL
                     RNRDHNGNGVPEYGATRDKAHNTDNGEMLFTVKKGNKEETQSGLNNYARVVEKGQYDS
                     LEIPAQVAASWESGRDDAAVFGFIDKEQLDKYVANGGKRSDWTVKFAENHSQDGTLLG
                     YSLLQESVDQASYMYSDNHYLAEMATILGKPEEAKRYRQLAQQLADYINTCMFDPTTQ
                     FYYDVRIEDKPLANGCAGKPIVERGKGPEGWSPLFNGAATQANADAVVKVMLDPKEFN
                     TFVPLGTAALTNPAFGADIYWRGRVWVDQFWFGLKGMERYGYRDDALKLADTFFRHAK
                     GLTADGPIQENYNPLTGAQQGAPNFSWSAAHLYMLYNDFFRKQ"
     gene            complement(22125..23195)
                     /gene="ygjJ"
                     /locus_tag="J1Y83_001395"
     CDS             complement(22125..23195)
                     /gene="ygjJ"
                     /locus_tag="J1Y83_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YgjJ"
                     /protein_id="HBA7629554.1"
                     /translation="MKLITAPCRALLALPFCYAFSAVAEEARPAEHDDTKTPAMTSTS
                     SPSFRFYGELGVGGYMDLEGENKHKYSDGTYIEGGLEMKYGSWFGLIYGEGWTVQADH
                     DGNAWVPDHSWGGFEGGINRFYGGYRTNDGTEIMLSLRQDSSLDDLQWWGDFTPDLGY
                     VIPNTRDIMTALKVQNLSGNFRYSVTATPAGHHDESKAWLHFGKYDRYDDKYTYPAMM
                     NGYIQYDLTEGITWMNGLEITDGTGQLYLTGLLTPNFAARAWHHTGRADGLDVPGSES
                     GMMVSAMYEALKGVYLSTAYTYAKHRPDHADDETTSFMQFGIWYEYGGGRFATAFDSR
                     FYMKNASHDPSDQLFLMQYFYW"
     gene            complement(23329..24762)
                     /locus_tag="J1Y83_001396"
     CDS             complement(23329..24762)
                     /locus_tag="J1Y83_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBA7629555.1"
                     /translation="MSDTKRNTIGKFGLLSLTFAAVYSFNNVINNNIELGLASAPMFF
                     LATIFYFIPFCLIIAEFVSLNKNSEAGVYAWVKSSLGGRWAFITAYTYWFVNLFFFTS
                     LLPRVIAYASYAFLGYEYIMTPVATTIISMVLFAFSTWVSTNGAKMLGPITSVTSTLM
                     LLLTLSYILLAGTALVGGVQPADPITVDAMIPNFNWAFLGVTTWIFMAAGGAESVAVY
                     VNDVKGGSKSFVKVIILAGIFIGVLYSVSSVLINVFVSSKELKFTGGSVQVFHGMAAY
                     FGLPEALMNRFVGLVSFTAMFGSLLMWTATPVKIFFSEIPEGIFGKKTVELNENGVPA
                     RAAWIQFLIVIPLMIIPMLGSNTVQDLMNTIINMTAAASMLPPLFIMLAYLNLRAKLD
                     HLPRDFRMGSRRTGIIVVSMLIAIFAVGFVASTFPTGANILTIIFYNVGGIVIFLGFA
                     WWKYSKYIKGLTAEERHIEATPASNVD"
     gene            complement(24825..25274)
                     /locus_tag="J1Y83_001397"
     CDS             complement(24825..25274)
                     /locus_tag="J1Y83_001397"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase subunit beta"
                     /protein_id="HBA7629556.1"
                     /translation="MRIIDNLEQFRQIYASGKKWQRCVEAIENIDNIQPGVAHSIGDS
                     LTYRVETDSATDALFTGHRRYFEVHYYLQGQQKIEYAPKETLQVVEYYRDETDREYLK
                     GCGETVEVHEGQIVICDIHEAYRFICNNAVKKVVLKVTIEDGYFHNK"
     gene            complement(25271..28363)
                     /gene="ebgA"
                     /locus_tag="J1Y83_001398"
     CDS             complement(25271..28363)
                     /gene="ebgA"
                     /locus_tag="J1Y83_001398"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311985.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase subunit alpha"
                     /protein_id="HBA7629557.1"
                     /translation="MNRWENIQLTHENRLAPRAYFFSYDSVAQARTFARETSSLFLPL
                     SGQWNFHFFDHPLQVPEAFTSELMADWGHITVPAMWQMEGHGKLQYTDEGFPFPIDVP
                     FVPSDNPTGAYQRIFTLSDGWQGKQTLIKFDGVETYFEVYVNGQYVGFSKGSRLTAEF
                     DISTMVKTGDNLLCVRVMQWADSTYVEDQDMWWSAGIFRDVYLIGKQLTHINDFTVRT
                     DFDEAYCDATLSCEVVLENLAASPVVTTLEYTLFDGEHVVHSSAIDHLAIEKLTSASF
                     AFTVEQPQQWSAESPYLYHLVMTLKDADGNVLEVVPQRVGFRDIKVRDGLFWINNRYV
                     MLHGVNRHDNDHRKGRAVGMDRVEKDLQLMKQHNINSVRTAHYPNDPRFYELCDIYGL
                     FVMAETDVESHGFANVGDISRITDDPQWEKVYVERIVRHIHAQKNHPSIIIWSLGNES
                     GYGCNIRAMYHAAKALDDTRLVHYEEDRDAEVVDIISTMYTRVPLMNEFGEYPHPKPR
                     IICEYAHAMGNGPGGLTEYQNVFYKHDCIQGHYVWEWCDHGIQAQDDNGNVWYKFGGD
                     YGDYPNNYNFCLDGLIYSDQTPGPGLKEYKQVIAPVKIHALDLTRGELKVENKLWFTT
                     LDDYTLHAEVRAEGETLATQQIKLRDVAPNSEAPLQITLPQLDAREAFLNITVTKDSR
                     TRYSEAGHSIATYQFPLKENTAQPVPFAPNNARPLTLEDDRLSCTVRGYNFAITFSKM
                     SGKPTSWQVNGESLLTREPKINFFKPMIDNHKQEYEGLWQPNHLQIMQEHLRDFVVEQ
                     SDDEVLLVSRTVIAPPVFDFGMRCTYIWRIAADGQVNVALSGERYGDYPHIIPCIGFT
                     MGINGEYDQVAYYGRGPGENYADSQQANIIDIWRSTVDAMFENYPFPQNNGNRQHVRW
                     TALTNRHGNGLLVVPQRPINFSAWRYTQENIHAAQHCNELQRSDDITLNLDHQLLGLG
                     SNSWGSEVLDSWRVWFRDFSYGFTLLPVSGGEATAQSLASYEFGAGFFSTNLHSENKQ
                     "
     gene            complement(28547..29530)
                     /gene="ebgR"
                     /locus_tag="J1Y83_001399"
     CDS             complement(28547..29530)
                     /gene="ebgR"
                     /locus_tag="J1Y83_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator EbgR"
                     /protein_id="HBA7629558.1"
                     /translation="MATLKDIAIEAGVSLATVSRVLNDDPTLNVKEETKHRILEIAEK
                     LEYKTSSARKLQTGAVNQHHILAIYSYQQELEINDPYYLAIRHGIETQCEKLAIELTN
                     CYEHNGLPDIKNVTGILIVGKPTSALRAAACALTDNICFIDFHEPGSGYDAVDIDLAR
                     ISKEIIDFYINQGVNRIGFIGGEDLPGKADIREVAFAEYGRLKHVVREEDIWRGGFSS
                     SSGYELAKQMLAREDYPKALFVASDSIAIGVLRAIHERGLNIPQDISLISVNDIPTAR
                     FTFPPLSTVRIHSEMMGSQGVNLVYEKARDGRALPLLVFVPSKLKLRGTTR"
     gene            29749..30081
                     /locus_tag="J1Y83_001400"
     CDS             29749..30081
                     /locus_tag="J1Y83_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-binding protein"
                     /protein_id="HBA7629559.1"
                     /translation="METVAYADFARLEMRVGKIVEVKRHENADKLYIVQVDVGEKTLQ
                     TVTSLVPYYSEEELMGKTVVVLCNLQKAKMRGETSECMLLCAETDDGSESVLLTPERM
                     MPAGVRIV"
     gene            complement(30123..31613)
                     /gene="ygjG"
                     /locus_tag="J1Y83_001401"
     CDS             complement(30123..31613)
                     /gene="ygjG"
                     /locus_tag="J1Y83_001401"
                     /EC_number="2.6.1.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311982.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine aminotransferase"
                     /protein_id="HBA7629560.1"
                     /translation="MITTFVFIPIFAIAAGVAQSLQYLNRYHVIREPPEHILNRLPSS
                     ASALACSAHALNLIEKRTLDHEEMKALNREVIEYFKEHVNPGFLEYRKSVTAGGDYGA
                     VEWQAGGLNTLVDTQGQEFIDCLGGFGIFNVGHRNPVVVSAVQNQLAKQPLHSQELLD
                     PLRAMLAKTLAALTPGKLKYSFFCNSGTESVEAALKLAKAYQSPRGKFTFIATSGAFH
                     GKSLGALSATAKSTFRKPFMPLLPGFRHVPFGNIEAMRTALNECKKTGDDVAAVILEP
                     IQGEGGVILPPPGYLTAVRKLCDEFGALMILDEVQTGMGRTGKMFACEHENVQPDILC
                     LAKALGGGVMPIGATIATEEVFSVLFDNPFLHTTTFGGNPLACAAALATINVLLEQNL
                     PAQAEQKGDMLLDGFRQLAREYPDLVQEARGKGMLMAIEFVDNEIGYNFASEMFRQRV
                     LVAGTLNNAKTIRIEPPLTLTIEQCELVIKAARKALAAMRVSVEEA"
     gene            31919..33439
                     /locus_tag="J1Y83_001402"
     CDS             31919..33439
                     /locus_tag="J1Y83_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HBA7629561.1"
                     /translation="MSSHPYVTQQNTPLADDTTLMSTTDLQSYITHANDTFVQVSGYT
                     LQELQGQPHNMVRHPDMPKAAFADMWFTLKKGEPWSGIVKNRRKNGDHYWVRANAVPM
                     VREGKISGYMSIRTRATDEEIASVEPLYKALNAGRTGKRIHKGLVVRKGWLGKLPSLP
                     LRWRVRGVMTLMFILLAAMLWFVAAPVVTYFLCALVMLLGNACFEWQIVRPIENVASQ
                     ALKVATGERNSVEHLKRSDELGLTLRAVGQLGLMCRWLINDVSSQVSSVRNGSETLAK
                     GTDELNEHTQQTVDNVQQTVATMNQMAASVKQNSATASAADKLSITASNAAVQGGEAM
                     TTVIKTMDDIADSTQRIGTITSLINDIAFQTNILALNAAVEAARAGEQGKGFAVVAGE
                     VRHLASRSANAANDIRKLIDASADKVQSGSQQVHAAGRTMEDIVAQVKNVTQLIAQIS
                     HSTLEQADGLSSLTRAVDELNLITQKNAELVEESAQVSAMVKHRASRLEDAVTVLH"
     gene            complement(33468..34091)
                     /locus_tag="J1Y83_001403"
     CDS             complement(33468..34091)
                     /locus_tag="J1Y83_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HBA7629562.1"
                     /translation="MSHHHEGCCKHEGQPRHEGCCKGEKSEHEHCGHGHQHEHGQCCG
                     GRHGRGGGRRQRFFGHGELRLVILDILSRDDSHGYELIKAIENLTQGNYTPSPGVIYP
                     TLDFLQEQSLITIREEEGGKKQIALTEQGAQWLEENREQVEMIEERIKARCVGAALRQ
                     NPQMKRALDNFKAVLDLRVNQSDITDAQIKKIIAVIDRAAFDITQLD"
BASE COUNT         8469 a         8655 c         8848 g         8121 t
ORIGIN      
        1 ctgattaaat ggtggcgaaa attaatcgat tattacggct cgggcctgtc ttacttctgt
       61 gtacatcatt tttcgcacaa tctaatatga ctgtctaccc tatggctgtt tccattaata
      121 gccagggaga aggaagtgtt cgtgtaatat ctaaaagtaa cgaagttcaa tacataaaag
      181 cgacagtatt ccgcatcgat aacccatcaa cgcctctaga aaacgaagtg gaaattaaat
      241 ccggtgatgc taatcatttg gttgttatgc cacctaaatt cgccttacca gccggtagta
      301 gcaaaaccgt gcgctttgtc gcaatggaac cagagcaaca agaaaaaaac tatcgtgtta
      361 agtttgaagc tgtacccagc attgatgatg ttgctaccga taaaaaagat ctctctatgc
      421 agttaacggt taatttaatt tggggcatcg ttgttagtgt tccacctcaa caaccagtta
      481 ctaaattaga aattaattca gcaaaaaaac tactcaatgc aggaaatcag cgtcttaaag
      541 tgttgacaat cgcttattgt aaaaataata gcaaagataa tagttgtaag actcagaccg
      601 taaataaaaa cattttccct ggtcaggaaa aaagtttaga aagcatatct ggctacgata
      661 aaatcgttgt caaatataat aactggatca ccaaggacaa tggcgagttt gagctggcgg
      721 tccattaatt atcgttgtga taaacaaagg aatagtcatg aaaaaggtat tcgtaaaatc
      781 tcttctggta gccgcaatgt tttccgttgc aggttctgca ttagcagtac aaaaagatat
      841 tactgtcact gctaatgtcg atgctgctct ggatatgact cagaccgata actctgcgct
      901 gccgaaagca gtagagatgc aatatctgcc gggtcagggt ctgcagtctt atcagttaat
      961 gaccaaaatc tggtctaacg acgtcactaa agatgtgaaa atgcagttag tttctccggc
     1021 gcaactggtt cagagcctgg acgccagcaa aattgttccg ctgactgtaa gctggggtga
     1081 tgaagagatt aaagctgatg cagcaaccac ttttagcgca gttaaaattt ttgcgtctga
     1141 tgctataacc aacggttctc tggctaaaaa cctgatgttt gctcagacca ccaaaggtgt
     1201 tctggaaaca ggtatctacc gtggcgtagt cagcatttat ctgtctcagg atatctaatc
     1261 tggatatctg aattaacagg taataagggg ggcaagtcgt ccccctttat tttaactatt
     1321 ctggtattta ttatggataa caggactgcc atggataaaa aattactggc tcttttgatc
     1381 ctggcgagtc tcagcccggc aaaggcgaca ttaaccaaaa ttcccgctgg ttttgaggtt
     1441 attgctcagg gacagcagga gtatatcgag gtttattttg cagggaaaag tctcggtaaa
     1501 tattatgcga tggttaatct tgataccgta acctttcttg attcatcaag tttatacagc
     1561 aaacttgaat tgagcactga cgatcaaaaa atcactcata cagtgaaaga aaaactatcg
     1621 cagcccttag ctcgccacgg tgaactggct tgtggttttg tacgtactga ttcaggatgc
     1681 ggttttctta atactgagac agtagaaata atctacaatg atgaagaaag ttcagtaacg
     1741 ctatttctta atccgcaatg gagttctgcg tttaactcga agtcattgta tttaaatcca
     1801 gacaaaaata cggttaacgc ttttatacat cagcaagaca ttaatgttct ggtacaggac
     1861 gattaccaat cgttgtcaat tcagggtaac ggtgcgctgg gaataacaga aaatagctat
     1921 attggcgcac actggaattt caacggttat gatgcagatg atgtcagcga caacaatgtc
     1981 gatgtcagcg atctctatta ccgctatgat tttttacgcc gttattatgt gcaggcgggg
     2041 cggatggaca accgcacatt atttaatgcg caaggtggaa actatacttt taacttcctg
     2101 ccactcggtg caatagacgg aatgcgtatc gggtcgacac tcagttattt aaaccagacg
     2161 caaagccagc agggaacgcc ggtaatgatc ctgctttcgc gcaattctcg tgttgatgct
     2221 tatcgtaatg agcaactgtt gggatcgttt tatctcaata gcggttcgca atttattgat
     2281 accagttcct ttccgccagg cagctatagc gtggcgttaa aagtctatga aaataaccaa
     2341 ctcacccgca ccgagcttgt gccgtttacc aaaactggtg gtctgacaga cggaaatgcc
     2401 cagtggttct tacaggcagg taaaaccaca tcacaggttt ctgatgatga aagttcagct
     2461 tatcagctag gggtacgcct gccattacat ccgcaatatg agctctacgc agggctggcg
     2521 aatgccgatg atgtaagtgc tttcgagtta ggtaataact ggacggcgga tttaggcgag
     2581 gcggggaatc tggccatcag cgccagcgtg ttccgtaacg atgacggcgg caaaggcgat
     2641 atgcaacagg ccaactggag tcatccggga tggccgacgt tgggttttta tcggaccaac
     2701 tctgacggtg atgcttgtac aaccgacaac agagagagct acaacgcctt aagctgttat
     2761 gaaagtattt ccgcgacggt ttcacagaat cttgtcggct ggaatatgat gctgggttat
     2821 acccgcacgc aaaataacac tgatgatagt ttgcgctggg acaaacagca gagctttgaa
     2881 aataactatc ttcgccagac tacggcacaa agtatttccg aaaccgtaca acttagcgct
     2941 tctcgcgctt ttgtgatgcg tgactggatt ttgagtactt cccttgggtt tttccatcgt
     3001 aacgacaacg gcagtggtag cgatgataac ggtttgtatc tgtcattttc gttatctgat
     3061 acgccgacga tggacagcaa taacaacagc cattcaacca atgtttctac ggattatcgt
     3121 tatagcgatc aggatggcga tcaaacgtca tggcagttat cgcatacctt ttataacgac
     3181 tcattcagcc ataaagagct tggcgtgacc gtcggcggtt tgaacaccga taccataaac
     3241 agcgcggtta acgggcgttg ggatggtcaa tatggaaatg tctacgctac agtctcagac
     3301 agttatgatc gccagaatca tgatcatctc tcggccttca ccgggacata cagctccacg
     3361 ctggcggtga gtcgctatgg catcaatgtg ggggccagcg gcacagatga tttgctgggg
     3421 gcggtgttgg tggatgtgaa aggtttctct gaacaggatg aacagagtca ggatctgcaa
     3481 ctcgaagcgc gggtggctgg cagcagaaca ttgcagcttg gtcaacgcga cagcgtgtta
     3541 ttcccctatc ctggatttca gtctggcttt gttgaagtta acgacagtaa tcagggtaac
     3601 cagcaaggaa caaccaacat cattaacggc gcgggaaatc gcgagttaat gctgttgccg
     3661 ggcaaattac gttatcgcga agtgtctgcc agctttaatt acaactatat cggtcgtttg
     3721 ttattgcccg catcagtaga gaagttcccg ctggtgggtc tgaatagcgc catgctactg
     3781 gtggctgaag atggcggatt tacactggag attaacggta gtgaaaaaga actgtatctg
     3841 ctttccgggc agcagttcct gaaatgtccg ctgagtgttt tgaagaaacg cgccagcatt
     3901 cgctatagcg gggatgtgaa ttgtagcgtg gtgagttatt cgcaactgcc ggagtctatt
     3961 caggttcagg cacagttaaa acagcctaaa ttacgtggaa acgtccagac ggcgcaaagg
     4021 gagattgcac catgataaat cgattgattg ccgtggtttt atgcttattt ggcatggtcg
     4081 cgtgggttca ggcaacaccc aatgtgacgg ctgaaattac ttatgatttg gccgtcggta
     4141 gggctgatta ttacttctgg aacgaggaag agtcggctgg gaataatgca tatatgtggt
     4201 atgaatgttc gtaccccaat ctccagcaaa cctgtacgaa ttctggaaat atctcgacag
     4261 tacaaattta tttaacggag caacgtagtg gtatgcgctg gccacttaag ctcaaaggat
     4321 ttaaagaagc tcatgtcatg aatcaggtac ctgggtgcga aggatatgtt gcaaaaacgc
     4381 accttcggga atctaatcgg tataaatgca tggcaactgg tgattcaacc atatattatg
     4441 ctgataaaaa gtttcttaca ctttaccttg aacaggcaga aatgaagaag ctgccaattg
     4501 gtggtgtctg gaaagggcag gttaaactgc attctaatag tcctgctcag gactattttg
     4561 cggatattac cctgaacacc ctcgatccca accatattga cgtattcttc ccggagttcg
     4621 cccacgctac gccacgggta caactggatt tgcacccaac aggcagcgtg aatgccagta
     4681 attatgccca ggatctgaca acgttggata tgtgcctgta cgacggtttt aacggtaatg
     4741 ccatcagtta tgaaattatg cttaaagatg aagggctacc cgcggcaggg cgcagagatg
     4801 gttatttttc catttatcgt cagggaagaa caacgactga cgatgtagag cgtatcgatt
     4861 acagagtaaa aatgtataac ccggaaacag gtgggcaaat cgacgtacgc aataatgaaa
     4921 atatggtctg gaacagcatc aacctgaagc gagttcgtcc ggtggtattg ccgggcattc
     4981 gttacgccgt aatgtgtgtg cctacgccac taaccctggc ggtcgataaa ttcagcgtga
     5041 tggataaaca ggcgggatat tacatgggta aattgtcggt catttttacc ccgtcattgc
     5101 cgacgatcaa ttagctctgt aggccggaca gacgttttcg cgtcgtctcc ggtaataaaa
     5161 acgctgaagc cgcctgaaaa ggcggcttcg tcattacaac ttacattaag tgttaatgtt
     5221 gcggtgcggg atcgttatgt gattcctgcg ccacctcaat cgccggtttg ttctgcaaca
     5281 cggtccagat aaccagcgca cctaacaggt cgaacactgc cagaactgcg aacagcgggc
     5341 tgaagccgat ggtgtcagcc agtgcaccga ctaccagtgc aaacagcgta cttgccagcc
     5401 atgcggacat cccggttaaa ccgttcgccg tcgccacttc gttacgaccg aacacgtcag
     5461 aagagagcgt aatcagtgca ccagacagtg cctggtgagc aaaaccaccg atacacagca
     5521 gcataatcgc gacatacggg ttggtgaaca gaccgatcat tcccgggcca atcatcagca
     5581 cagcacccag cgttacgacc atcttacggg aaacgatcag gttcacacca aaccaacgct
     5641 ggaacagcgg cggcaggtaa ccaccgagga tacaaccgag gtcagcaaac agcatcggca
     5701 tccaggcaaa catcgcgatc tctttcaggt taaagccgta aactttaaac atgaacagtg
     5761 ggatccacgc gttaaaagta ccccatgccg gttctgccag gaagcgcggc aacgcgatgc
     5821 cccagaactg acggttacgc aggatctgac caacggacat tttcttcgct gtgtcaacct
     5881 ggtgctgggc ttcctgacca ttaataatgt agtcgcgttc ttcatcggtc agatgtttct
     5941 ggtcgcgcgg atgtttatag aaaatcagcc atgccatcgc ccagataaag ctcaacgcac
     6001 cggagatgat aaatgccatc tgccagctgt gcatcacgat tgcccatacc accagcggcg
     6061 gcgcaatcat cgcaccaatc gaagaaccta cgttaaagta acctaccgcg atggaacgct
     6121 cttttgccgg gaaccattcg gaactggctt tcagacccgc cggaatcatc gcggcttccg
     6181 cggcaccgac cgcaccacga gcaaccgcca ggccacccca actacctgcc agcgcagttg
     6241 caccacagaa cacggcccac agtacagcaa acattgcata accgattttc gtacccagca
     6301 catccagtac ataacccgct accggttgca tgaccgtata agcagcagaa tacgctgcga
     6361 tgatatagga atactgttgg gtggagatgt ttaactcttc catcagagtt ggcgcagctg
     6421 ccgccacagt gttacgcgtc aggtaaccaa gcacggtgcc gagcgtcacc agtgcgatca
     6481 tataccaacg taacccttta attttacgca tgtaaaacct catcgttttg ttatatccgc
     6541 aggaagcgcg gccatcatcc cgccagaggc tgaagatgtt agttacgacc gggtaaccga
     6601 aaaaaaaccg gcacgccccg aacgttgcca tgaaagtttc gtgctaattc ggcttccgta
     6661 ccggttaacc caattcatct aacaaatgaa tgaatcggca atttgcattc cgtcgactta
     6721 tgatttgcga cggcagaaag ataacttgtc atacaacttt aaaaggtgag agccatcaca
     6781 aatgtgcgaa tatttgtagg gacattacct gacgacggcg aggccagtgc tggcgcggcc
     6841 tgcagcgaga tttaccactt tgagagtaat ttttttaact acgtttattg atctaactca
     6901 cgaaaatatc ttcggactct ggaaattggt gtgataactt tgtcagcatc gcaccataag
     6961 caagctagct cactcgttga gaggaagacg aaaatgactc cgtttatgac tgaagatttc
     7021 ctgttagata ctgaatttgc ccgccgtctg tatcacgact acgcaaaaga ccagccgatt
     7081 ttcgattacc attgccattt gccgccgcag cagattgcgg aagactatcg ttttaaaaac
     7141 ctgtatgaca tctggttgaa gggcgatcac tacaaatggc gcgctatgcg taccaacggt
     7201 gtggccgagc gtctgtgcac cggtgatgcg tctgaccgtg aaaaatttga cgcctgggcg
     7261 gcgactgttc cgcacactat tggcaacccg ttataccact ggacgcacct cgaactgcgt
     7321 cgtccgtttg gtatcactgg caaattgctt tctccgtcaa ctgccgatga aatctggaac
     7381 gaatgtaacg aactgctggc gcaggataat ttctctgcac gcggcatcat gcagcagatg
     7441 aacgtgaaaa tggtcggcac caccgatgac ccgatcgatt ctctggagca tcacgcagag
     7501 atcgccaaag acggctcttt caccattaaa gtgctgccga gctggcgtcc ggacaaagcc
     7561 tttaacattg aacaggcgac ctttaacgac tacatggcga agctgggcga agtttccgat
     7621 accgacattc gccgctttgc tgacctgcaa actgccctga ctaaacgtct ggatcacttc
     7681 gccgctcacg gctgtaaagt gtctgaccac gcgctggatg tggtgatgtt tgctgaagcg
     7741 aacgaagcgg aactggacag catcctcgcg cgccgtctgg caggcgaaac cctgagcgag
     7801 cacgaagtgg cacagttcaa aactgcggtg ctggtgttcc ttggtgccga atatgcacgt
     7861 cgcggatggg tacagcagta ccacattggc gcactgcgta ataacaacct gcgtcagttc
     7921 aaactgctgg ggccggatgt cggctttgac tccatcaacg accgtccgat ggcggaagag
     7981 ctgtctaagc tgctgagcaa gcagaacgaa gaaaacctgc tgccgaaaac cattctctac
     8041 tgcctgaacc cgcgcgataa cgaagtgctg ggcaccatga tcggtaactt ccagggcgaa
     8101 ggtatgccgg gcaaaatgca gttcggttcc ggctggtggt ttaacgacca gaaagacggt
     8161 atggaacgtc agatgaccca actggcgcag ctcggtctgc tgagccgctt tgtcggcatg
     8221 ctgactgaca gccgtagctt cctgtcatac acccgtcacg aatacttccg ccgcattctg
     8281 tgccagatga tcggtcgctg ggtggaagca ggcgaagcac cggcggacat caacctgctg
     8341 ggcgagatgg tgaaaaatat ttgctttaac aatgcgcgtg actacttcgc cattgaactg
     8401 aactaaggtc tggggttgat atgcaataca tcaagatcca tgcgctggat aacgtcgcgg
     8461 ttgctttagc ggatttggct gaaggcacag aagtcagtgt cgataaccag actgttacgc
     8521 tgcgccagga tgttgctcgt ggacataaat ttgcgttaac ggatatcgca aaaggggcca
     8581 acgtcattaa atatggcctg ccgattggtt atgcattggc ggatattgcg gcgggggaac
     8641 acgttcacgc ccacaatacg cgcacgaatc tgagcgatct ggatcagtat cgctatcaac
     8701 ctgattttca ggatctgcct gcgcaagcgg cagatcgtga agtgcagatc tatcgtcgcg
     8761 ctaacggcga tgtcggggtg cgtaatgagc tgtggatcct gccaaccgtg ggctgtgtca
     8821 acggcatcgc gcggcagatc caaaatcgtt tcctgaaaga gaccaacaac gctgaaggta
     8881 ccgacggcgt gttcctcttc agccacacct acggctgctc acaactgggc gacgatcaca
     8941 tcaatacccg caccatgctg caaaacatgg tgcgccaccc gaacgcgggc gcagtgctgg
     9001 tgattggtct gggctgtgaa aacaaccagg ttgccgcatt ccgtgaaacg ttgggcgata
     9061 tcgatcctga acgcgttcat ttcatgatct gccaacagca ggatgatgaa atcgaggccg
     9121 ggatcgcgca tttgcatcag ctgtatagcg tgatgcgcaa cgataaacgc gagccgggca
     9181 aactcagcga actgaagttt ggtctggagt gcggtggttc tgacggtctt tctggtatta
     9241 ctgctaaccc gatgctgggg cgtttctctg actacgtgat tgctaacggc ggtactaccg
     9301 tactgaccga agtgccggag atgtttggcg cagagcagtt gctgatggac cattgtcgcg
     9361 acgaagcaac gtttgaaaaa ctggtcacca tggtcaatga cttcaagcag tactttattg
     9421 cccatgacca gccgatctac gaaaacccat caccggggaa caaagcaggc ggtatcacca
     9481 cgctggaaga taaatcgctt ggctgtaccc agaaagcggg ttccagtgtc gtggttgacg
     9541 tgctgcgtta cggcgagcgt ctgaaaacgc cggggctgaa cttgttaagt gcgccgggta
     9601 acgatgccgt agcgaccagc gccctggcgg gtgcgggctg ccatatggtg ctgttcagta
     9661 ccggtcgcgg cacgccgtac ggcggctttg taccgacggt gaaaatcgcc accaacagcg
     9721 aactggcggc gaagaaaaaa cactggatcg actttgacgc gggtcagctg atccacggta
     9781 aagcgatgcc gcagttgctg gaagaattta tcgacaccat cgttgagttt gccaacggta
     9841 agcaaacctg taacgagcgt aacgacttcc gcgaactggc gatcttcaaa agcggcgtaa
     9901 cgctataaag tcgaaatgag ttgttaataa aaaacggcgt ttcataatgt gaagcgccgt
     9961 ttgcttttcc atacctgcta aggatttatc atgaccgcgt attggctggc ccagggcgtg
    10021 ggtgtcatcg cctttctgat tggtatcaca acatttttca atcgtgacga acgtcgcttc
    10081 aaaaagcaac tttcggtcta tagcgccgtt attggcgtac atttttttct tctgggcacc
    10141 tatcctgccg gtgccagtgc catccttaat gccattcgta cattgattac cttacgcacg
    10201 cgcagcttat gggtaatggc gatttttatt gtgctgactg gcggaattgg cctcgcgaag
    10261 ttccatcatc ctgtcgaact attgccggta atcggcacga ttgtcagtac ctgggcgctg
    10321 ttccgctgta aagggctgac catgcgctgc gtaatgtggt tttcaacgtg ttgctgggtg
    10381 attcacaact tctgggcggg gtcgataggc ggcacgatga ttgaaggcag ttttctgctg
    10441 atgaatggcc tgaatatcat tcgtttctgg cgtatgcaga gaaggggaat tgatccgttt
    10501 aaagtggaga aaaccccttc cgccgtagac gaaaggggct aagtattagt tacgcagggc
    10561 gctatttgcc agacgatcgt cttccgcctg gcaagctgcc gcagtgaaca gcacgtcagt
    10621 tgacgagttc agcgcggttt cacaggagtc ctgcaatacg ccgataataa agccgacagc
    10681 aaccacctgc atggcgatat cgttcgaaat accgaacata ttacaggcca gtgggatcag
    10741 caacagagac ccccccgcca cgccagatgc gccacaggca cacagagaag ccactacgct
    10801 caacagcagc gctgtgggca gatcaaccgg aatacccagc gtattaaccg cagccagcgt
    10861 caacacggta atggtgattg ctgcgcccgc catattgatg gtggctccta gcggaataga
    10921 aacggaatag gtatcgcgat ccagattcag cttttcacac agcgccatat tcaccggaat
    10981 gttcgccgca gagctgcggg tgaagaaggc atacacgccg ctttcgcgca ggcacagcag
    11041 caccagcggg aacgggttac gacgaatttt ccaccatacc agcagtgggt taaccaccag
    11101 cgccaccagc agcatacagc caaccagtac caccagcagt tgcgcgtagc cccacagtgt
    11161 ggagaaaccg gtggttgcca gggtagaaga aaccagacca aaaataccga tcggtgcgaa
    11221 gcgaatcacc agtttcacca taaaggtaac ggcattcgac atatcgttga ccaggttttt
    11281 ggtggtttcg ttaccgtgac gcagtgcgaa accgaggcca attgcccaca ccaggatccc
    11341 gatatagtta cctttcagca gcgcgtcaat ggggttggaa accatgctca ttaccagccc
    11401 gcgcatcact tctacaatgc ctgacggcgg cgaaatatca cccgcgctac tggacaagtg
    11461 cagggtagaa gggaaggcaa aactgaagat tactgcggcc agagcagcag agaaggtgcc
    11521 cagcagatag aggaacaaaa tagggcggat attggttttc tgcccgtgct ggtggttagc
    11581 aatagatgcc attaccagca tcaacaccag gatgggggca acggctttca gtgcgccgac
    11641 gaacaaagta cctaacagac caacagcttc cgccgcgggt tttgagatcc atgccagaag
    11701 aatccccaga ataaggccgg ccaggatttg ttttaccagg ctgccatgag ccagacgccg
    11761 gaatagcccc ggtgaacgtt gcgtagtcat ttttcgatcc tttcattgtg ttgtcgcaca
    11821 tccctggtgc gttctgtcga cgcatctttc aggatgtaac aaagtgtttg caaggtcgag
    11881 tataaggaaa gttacagctt cgggaagcga aaaatgctgg attttaaaga gaggtcatat
    11941 ttcgcaactt aatggttaca tttgtttgac ataaaaataa agcccaacgt gcggcttcca
    12001 gactgatgac aacgtcaaaa aagtactcgg tcagtcccct cgcccctttt ggggagaggg
    12061 agaaaacggt actcatgttg atgacagggt acacggctta ggcaggatta aaaattattc
    12121 tacccgctgc ttatcatgcc gataattcac ccaggcgttg ataataaacg tcatcaccag
    12181 aatgccaaac accacgccca gcgagacggc gatcggaata tggtagaagt cgacaatcag
    12241 catcttgata ccgataaaca ccagaatcac cgccagacca tatttgagca tcgagaaacg
    12301 ctctgccaca cctgccagca ggaaatacat cgcacgcagg ccgaggatcg caaacaggtt
    12361 tgaggtcagc acaataaacg gatcggtggt tacggcgaag atagccggaa tactatccac
    12421 ggcgaaaatc acgtcgctca actccaccag aatcagcacc agcatcagcg gcgtggcata
    12481 caacaaacca ttcttacgca caaagaaatg ctcgttgtcg atggtgtcgg tcatgcgcaa
    12541 atgaccgcgt agccagcgca ccagcggctt gtcgccaatg cctgattcat cttcatgggc
    12601 gagcgccatc ttcacgccgg taaacagcag gaaggcaccg aagatataca agatccagtc
    12661 gaactgtgaa atcagccagc taccagtgaa gatcatgatg gtacgcagaa caatcgcccc
    12721 gagtacgcca tacaccagca cgcggcgttg taatgccgcc ggaacagaga aatagctgaa
    12781 caacatcagc cagacaaaga cgttatcgac cgccagcgat ttctcaatca gataacccgt
    12841 gagaaaggcc agtgcctgtg gatcggcaac ggcgcgacct tcggtttgca ccagatacca
    12901 ccagaaagcg gcgttaaaca gtaacgacag cgtcacccag accagcgacc aggccgcagc
    12961 ctgtttcatg gtcatggcat gtgccccacg acgcccctgc aacaacaggt cgatagccag
    13021 cataatggtg acaacaacag cgaatccgcc ccatagcaac ggcgtgccga cagtattcat
    13081 agaagttcct tacacataaa aaaacggcca acgtcggaag acatcagccg ctgcttttta
    13141 tgcatagacc tcgccttccg gcaaggtctc acttacaaca aaaaaggaat acgtctgcgt
    13201 attcctttcg ggttgcccgg tgaccggatg tggtttttca cacatcgtaa tgacgatcga
    13261 ccgacaatga agttactccc ctttgcaggt aacaaagtat gacagaccat tcagtcagtc
    13321 aatggtctgt tacatacctt ttacactgtt ttactttggc aattatagtt ttacgttatc
    13381 tgccggaaaa atcaccccag tctggcggcg gatctcagtc agcagtttgg cggtgatacg
    13441 actgaccgcc agccctggat gattcaccag atgctcatcc accagggtag cgaacagctc
    13501 tgcttcgtag agcatggtat taatatgctg tggctgggtg agatcctgca tctggctgcc
    13561 acgcggtaca aagcagactt tctggcattc agagagtttt tcaatcacca gcgctcccgc
    13621 ttcgccctga atctcgcttg ccaggacaga atcgctcact tttgagtgct gcaacgtaac
    13681 gctgaaatca ccgtaatcca tcaccaccac gccgtgggca tccacaccac ttgccagcaa
    13741 actggcggtt gcctgcacgc ttttcggttc accgaataat gccaccgccg acgccaggca
    13801 gtaaaagccg atatccataa tcgaaccgtt ggagaatgcc ggattgaagg tgttgggatt
    13861 ctctccgtcc aggtaacgct gataccgcga ggaatactgg caatagttga aaaagacttt
    13921 acgcagtttg ccgactttcg gcagcgcctg gcgtaacaga tgaaagttcg gcaggtaggc
    13981 cgttttaaat gcctcaaaca gcaccacctg attttcccgc gcacaggcaa tggcggcatc
    14041 cacttccgcc agattcgacg ccagcggttt ctcgcaaatc acatgaattt tgtgactaag
    14101 gaaaagttgt gtctgggaaa agtgcaggga attcgggctg gcaatataca ccgcgtcaat
    14161 ggcatcgctt tctgccatcg cttccagcga cgtaaacaga tgctcgacag aaaaatcatt
    14221 ggcgaagtgc tgggcctgtt caagactgcg ggaataaacg gcggttaact tgtatttacc
    14281 gctctcatgg gcggcctcga cgaactggcg agtgatccag ttcgtaccaa tcacagcgaa
    14341 acgtatcata acgcgtggat actccataaa gggattctct cgtcaatgta gcacgccttt
    14401 ttcatcttca acgtgcgcag atacgcatta ttctttcgcc ggacagccat gctcactaaa
    14461 cggcccaatc ataaccaacg gcccgagata tttcggctgg cgatggagga tttttgcgtc
    14521 aatcaccgcg ctgacacgag cgagtttctc ccgcagttga gtataaaaac cgtgcttcat
    14581 attgaggtct ggcgcgttaa aaccgatagc gtcgatgccg tattgctttg ccagccagat
    14641 ggcgcgttga ttatgaaatt cttgcgagat aatggtgata tggttttcac caaacacctt
    14701 tttggcacgc accaccgaat caagcgttga gaatccggca tagtcgcaga agatcacttt
    14761 tgctggtacg cctttagcga tcaaggcctg ctgcattcct gacgcttcat cgtaattttt
    14821 acgtccgtta tcgccactaa ctaacagcca tttcactttt ccggcgtggt acaactctgc
    14881 tgcggtatca atacgtcggg taaagtagcg attacccggc ctcgccccca gcaataaacc
    14941 cacgttccgc gccggaacgg cgttgacatc gctccaggtc agttgttggc tggcgttgac
    15001 cataacgcga tcggcaataa aaagggttgc tcctgctata agcaacaata aacagccaat
    15061 cttaagagtc cggcgagaga aacaaacgcg gcgaagaagg tgggcaaata cgcgcagcat
    15121 ggtcgttatc cttaacgaaa ggggggaggt cgattttagg cgctaaagga ggaaaaaata
    15181 acgcctgaca tcattttcag attttgtcct gcccaagcga caactgcgtt agccacagcc
    15241 gggtgtcgaa ctcaagctgg tggtactttt gctttcagct tgccgccctg cacacgcgaa
    15301 acggcagtat gtaagccgag cgcgtttttc agcacatgga tcttgttgtc atacatcact
    15361 ttagtgatcg gcggggcatt gcgcagaaac tgatttttaa gatcctgagt atattgccag
    15421 agggctttat cagtggcgta gtcgtgtgtt cccggataac gttttgccag cacatcgccc
    15481 agacgctgtt cgttaatcag cgttcgcacc tgggaaagta gctgctcggg ataaccctga
    15541 agataagtaa gattgctcat aaccgcccac gaaaaagaga aaaagggtat actcacgcac
    15601 ccttttcagg ggcaacgtcg aaattttatc attcaggagg gccgatgagc cacttagaca
    15661 acggtttccg ttcactgaca ctacaacgtt ttccggcgac ggatgacgtt aacccgctac
    15721 aggcgtggga agcggcggat gaatatttgc tgcaacagtt ggacgacaca gaaatccgcg
    15781 gcccggtgtt gatcctgaat gatgcctttg gtgcgttaag ttgtgcgctg gcggaacata
    15841 agccatacag cattggcgac tcatacatca gcgaactggc gacgcgcgag aatttacgcc
    15901 tcaacgggat tgatgagtcg agcgtgaagt tcctcgacag caccgccgac tacccgcaac
    15961 agccgggtgt ggtgctgatc aaagtgccga aaacactggc attgctggaa caacaactgc
    16021 gcgcgctgcg caaagtggtc acgccagata cgcgcattat tgccggtgcc aaagcccgtg
    16081 acattcacac ttccacgctg gaattgttcg aaaaagtgct cggtccgacc accaccacgc
    16141 tggcatggaa gaaagcgcgc ctgattaact gcaccttcaa cgaaccgccg ttggccgatg
    16201 cgccgcagac cgttagctgg aagctggaag gcactgactg gactatccac aaccatgcga
    16261 atgttttctc ccgcactggg ctggatattg gcgcgcgctt ctttatgcaa catctgccag
    16321 agaatctcga aggtgagatt gtcgatctcg gttgcggtaa tggcgttatt ggtctgacgc
    16381 tgcttgataa aaacccgcag gcgaaagtgg tgtttgtcga tgaatcgccg atggcggttg
    16441 cttccagccg tttaaacgtt gaaaccaaca tgccagaggc gttggatcgc tgcgagttta
    16501 tgatcaacaa cgcgctctcc ggcgtggagc ctttccgttt taatgctgtg ctctgcaacc
    16561 cgccgtttca tcagcaacat gcgctgaccg ataacgtcgc ctgggagatg ttccaccatg
    16621 cccgccgctg cctgaaaatc aacggcgagc tgtatatcgt tgccaaccgt cacctggatt
    16681 acttccataa actgaagaaa attttcggca actgcaccac catcgccacg aataataaat
    16741 ttgtggtgct gaaagcagtg aagctggggc gtcgtcggta agattggggg ttacctgccg
    16801 gatgcggcgt aaatgcctta tccggcatac gttcagcaca ttacccccag gtcggataag
    16861 acgcggcaaa cgtcgcatcc ggcaatgtct gcccagttct ggaatcgcgc ttccataaaa
    16921 caaaaaatcc ctgtcaccat cctctacatt ctcttgttta gcgttgttca acgtttatgc
    16981 tacatacgtg aagttatgac gcataaagaa tacgatttct ttaccgccgt tcatcgtact
    17041 aaggggaaaa aatgattgcg attgccgaca tcttgcaagc aggagaaaag ctaactgctg
    17101 tggcaccttt tctggcgggt attcagaacg aggaacaata cacccaggcg ctggaactgg
    17161 tagatcatct gctgctcaac gatcctgaaa accccttgct ggatctggcg tgtgccaaaa
    17221 taaccgcgtg ggaagaatca gcgcccgaat ttgctgaatt taatgccatg gcgcaagcca
    17281 tgcctggcgg tatagctgtg attcgtaccc ttatggatca atatggttta accctttccg
    17341 atcttccgga aattggcagt aaatcgatgg tgtcacgcgt tttgagcggg aagaggatat
    17401 taacgctgga acacgctaaa aaactggcag cgcgatttgg tatttctccc gccttgttta
    17461 ttgattaacg cggcgtgccg gatgcggcgc atccagcact tcaaacaacg atttaaatct
    17521 ccagcgccaa ccgtgttccc tgggcaattg cccgtcgtgc gtccagctcc atagccacat
    17581 cgcagccgcc aattaaatgc acggttttcc cgctatcaat gagcggttgc gccagcgtgc
    17641 ggtttggctc ctgccccgcg cagatcacca catgatctac cgccaaaatt tgcggttcgc
    17701 cgttgatcac cacatgcagc ccttcatcgt cgatcttctg gtaacttacg cctgggatca
    17761 ttttcacgcc gcgtgagagc agggtggtgc gatggatcca gccggtggtt ttgcctaacc
    17821 cttgtcctgg tttgctggct ttgcgctgga gcatcacaat ctgccgtggg ctacggggga
    17881 tttgcatgcc ttgcggactc aaacccccgg cctgttgcag gctactgtcg attccccact
    17941 cattacagaa cccggcgata ttctggctgg tggattcgcc cggctgactt aaatacatcg
    18001 ccgtatcaaa accaattccg ccacaaccga tgatggcaac tttgttgccc accggcgctt
    18061 tgtcgcgcag tacatcgaga taactcaata ccttcggatg atcgatcccg tcgataggcg
    18121 gaacgcgcgg cacgatccca ctggcgagga tcgtttcatc gaacgcctgt aactgatccg
    18181 ctgtcacggt gtgattgagt tttagcgtca cacccgtcac ttcgatcatc cggcggtaat
    18241 agcgcagcgt ttcgtaaaac tcctctttgc cggggatctg tttggcgata ttaaactgcc
    18301 cgccgatctc gctgtgcgca tcaaacaatg ttacctgatg cccacgcgcc gccgcgttaa
    18361 tggcaaacgc cagcccagca ggtcccgcac cgaccaccgc cagatttttt ttctgcacgg
    18421 caggaaggat tggcattttg gtttcatggc aggcgcgagg attcaccagg cacgaggtga
    18481 ctttgccaac gaagatctga tcgagacagg cctggttgca gccaatacaa gtgttgatct
    18541 catcggctcg tcccgattgc gcttttgaca gcagttccgc atcagcaaga aacggtcgcg
    18601 ccatcgatac catatcggca tcgccgcgcg agagaatatc gtcggcaacc tgcggatcgt
    18661 taatccggtt ggtggtcacc agcggcagcg aaacgtggcc tttcagtttg cgcgtgaccc
    18721 agctaaaggc accgcgcggc acgggcgtgg caatggtcgg aatacgtgct tcatgccagc
    18781 caatgccggt gttgataatg gtcgcgcccg ccgcttcaat ggcctgcgcc agctctacca
    18841 tttcggcaaa agtaccgccg ccttcgacca ggtcgagcat cgacagtcgg tagataataa
    18901 tgaagtcgtt gccgacacgt tcgcgcaccg cacgcactac ttctacggca aaccgcatcc
    18961 ggttgcggta atcgccgccc cactggtcac tacgctgatt ggtgcgcagc gtcagaaatt
    19021 cgttgatcaa atacccttcg gaccccatca cctctacacc gtcgtatctt gcctcccgtg
    19081 ccaattgcgc gcagtgggcg aaatcgtcga tcagttgcag gatctcttca tgggtgagtt
    19141 cgtgaggaac gaaacggttg atgggggcct gcaatgcgga gggagcgacc agatgcggtt
    19201 ggtagctgta gcgcccggta tgcaaaattt gcagggcgat tttgccgcct tcctgatgta
    19261 cggcttcggt aatggtgcga tggtgtggga tctggttggc gtcattgagc attgcaccgc
    19321 cttccatgcc aacgcctgtt aaatctggtg cgataccgcc gctgacaatc aacgccacgc
    19381 cgtgacgggc gcgttcggca taaaacgctg ccagccgttc agcgccgttc gggtattcct
    19441 ccagcccggt gtgcattgag cccatcaaca cgcggttttt taacgtggta aaacctaaat
    19501 ccagcggggc gaacagcgac gggtagctca taaaattgtc cattatgtaa aataattgtt
    19561 atgtggtcgg atgagttcta atttagccgt cgccggagaa aagggaaaag gggagtgcgg
    19621 ggtttgtgat gggattcaaa aagaatacgc ccggtggtaa cgccggatgc gacgcttgac
    19681 gcgtcttatc cggcctgggg gattgcacat gtagggcgga taaggcgttt acgccgcatc
    19741 cgccagccgt tgctaattac tgcttacgaa aaaaatcgtt atacaacata tacaaatgcg
    19801 cggcactcca ggagaaattt ggtgcgcctt gctgcgcgcc agtgagcggg ttgtaattct
    19861 cctgaatcgg gccatcggcg gttaaccctt tggcgtgccg gaagaacgta tcagccagtt
    19921 tcagggcgtc atcgcgataa ccgtaacgct ccatcccttt cagaccaaac cagaactgat
    19981 ccacccatac gcgcccgcgc cagtagatat ctgcgccaaa ggctgggttg gttaaggctg
    20041 ccgtccccag cgggacaaag gtgttgaatt ctttcggatc cagcatcacc ttcaccaccg
    20101 cgtcggcgtt ggcctgcgtt gccgcaccgt taaacagcgg tgaccagcct tccggccctt
    20161 taccgcgctc gacaatcggt ttgcccgcgc agccgttcgc cagcggttta tcttcaatac
    20221 gcacgtcata gtagaactgt gtagtcgggt cgaacataca ggtgttgatg tagtccgcga
    20281 gctgttgtgc taactgacga tagcgtttgg cctcttccgg cttaccgaga atcgtcgcca
    20341 tctccgccag ataatggtta tcgctgtaca tatagctggc ctgatccacc gactcctgca
    20401 atagcgagta gcccagcaac gttccgtcct gactgtggtt ttccgcgaat ttcaccgtcc
    20461 agtcgctacg tttgccgccg ttagcgacat atttatccaa ctgttctttg tcgataaacc
    20521 caaagacggc ggcatcatca cgccccgatt cccacgacgc cgcaacctgt gccggaattt
    20581 ccagactgtc gtactggcct ttctccacca cgcgggcgta gttgttcagc ccagactgcg
    20641 tctcttcttt attgcctttt ttcactgtaa acagcatctc gccattatca gtattgtggg
    20701 ctttatcgcg ggttgcgcca tattccggta cgccgttgcc gttgtgatcg cggttacgta
    20761 gccaccagtc gtgataggcc accagtttcg ggtacatctc tgccagccag gttttatcct
    20821 gggtgacgtt gtacacttcc atcaccgacc aggcggcaag gctgggtttg gtgttacgtt
    20881 cgttccagtt gccgccatcg ccgccacgct cggtgctaag gttccacgct atcaggtcgg
    20941 ggacaaagcc cacatcctgc ggacgcacgc tgtcgcctgg ctggatctgc caggagaaga
    21001 ccgcgcggat attctctttg gcgatatccg gattgaaatg cgccatcgca aacgcctgct
    21061 tccaggtatc ccacggccag gtctgattgc cggagaacca gcgcccggtc accgacggcg
    21121 tgacggtgtt atatttcacc gcaccgcccg gcgatcgcca gttaccgttg agcgtttcga
    21181 tggctttcac cgcgacgcgc gtctgttccg gcgtcgcatc cggattggtt aaccctttct
    21241 tcagatactc ttcccagcgt tgctgcgagg cggtgagata aaacgccgga cgcgccagaa
    21301 tatcgcggat ctgcatttgc tctttgctaa cttcctgagc ggtcagcaga tgggagtagg
    21361 tggtatagag cgtcgtcgaa ccgttgatat gcgccttact ggtaaagcga ttgccgttga
    21421 tttcagtctg cactggcagg gatttatgca cctgatattc tgattcaccg gaggtcagca
    21481 gatcccaggt ggcgcgcact ttgccaaagg tgactttcag gccatcacgg gtggcgctga
    21541 ttttgcgctg atagtcaggg tattcgccag caatggtttt atcggaaagc ggtttccctt
    21601 ctttcgcttc cagtttttcc agcagttcac catcccacac cagatccagc ggtttatcgc
    21661 tggtgatttt ggtttccagt agtgatgtgc gcggcgtggc gaagcgaaga gtcatttcga
    21721 cctgcacatc ttttgctgtc agtttttgca ccagcgcgcc gggaatactg tatgcctcca
    21781 gcgtgaagtc gactttcttg ccgtcctgcc agacggtcag gcggtcgaaa ttgctggcca
    21841 taaagttgat gtactcttcc gtcagcagcg caacgcccgg aaagccgccc atggtgttcg
    21901 ggccgtctgg caacagatga ccatgccagg ctccgagatc gaaaaacgga ttaaaacgct
    21961 ggtgatcgtc gtaatcgtaa tccttcatgt actgcggcgc gccagtacgg ttaatcacgt
    22021 ttttataatt gtcagcgtta gcggcatggg cggaaaagct catcagcaga gcacaggcta
    22081 ctggcgttaa aatggttttt attttcatga gattactgtc cttattacca gtaaaaatat
    22141 tgcatcagga agagttggtc gctgggatcg tgagaggcat ttttcatgta gaagcggcta
    22201 tcaaaagccg ttgcgaaacg tccgccgccg tattcgtacc agataccaaa ctgcatgaaa
    22261 gaggtggttt catcgtcagc gtggtcaggg cgatgtttgg cgtaggtgta agcggtggag
    22321 agataaacgc cctttaacgc ttcatacatg gcgctcacca tcatcccgct ttcacttccc
    22381 ggtacgtcca gcccgtcggc gcgtccggta tggtgccagg cgcgagcggc aaagttaggg
    22441 gtaagcaggc ccgtgagata gagttggcct gtgccgtcgg tgatttccag accattcatc
    22501 caggtgatac cttctgtaag gtcatactgg atgtaaccgt tcatcattgc cggataggtg
    22561 tatttgtcgt cgtagcgatc gtacttgcca aaatgtagcc aggctttgct ttcgtcatga
    22621 tgtccggcag gtgtggcggt aacgctataa cgaaagttgc cgcttaagtt ctgtaccttc
    22681 agcgcagtca taatgtcgcg ggtattggga atgacgtagc ccagatcggg cgtgaaatcg
    22741 ccccaccatt gcaggtcatc cagcgaggaa tcctgacgca gactgagcat gatttcggtg
    22801 ccatcattgg tacgataacc gccatagaaa cggttaatcc caccttcgaa gccaccccag
    22861 ctatggtcag gcacccaggc attaccgtcg tggtcggcct gtacggtcca gccttcgccg
    22921 taaatcaggc cgaaccagga gccgtacttc atctccaggc caccttcaat ataggtgccg
    22981 tcgctgtatt tatgtttatt ctcaccctct aaatccatat atccaccaac ccctaattcg
    23041 ccgtaaaaac gaaatgaagg agaagatgtc gaggtcattg cgggggtttt tgtgtcgtca
    23101 tgttctgccg gacgtgcttc ttcagcgacc gcagaaaagg cgtagcaaaa cggcagagca
    23161 agtaatgctc tgcatggtgc agtgattaat ttcattgtgt taatccatga gctaaagaaa
    23221 acgtacagcc ttttatcgtt gtaattataa aaatataaag gcagaaacta ttttccctct
    23281 ccccatccag gaagagggaa aatgcctgct cattttttat tattctgctt aatcaacatt
    23341 gctggctggc gtcgcttcaa tatggcgctc ttcagccgtt aatcccttta tatatttact
    23401 gtatttccac cacgcaaagc cgaggaagat aacaataccg ccgacgttat aaaaaatgat
    23461 ggtcagaata ttcgcgccag tcgggaatgt cgaagcgaca aaccctacag caaatatcgc
    23521 aatcagcatt gaaacaacga taataccggt gcgtcgggaa cccatacgga aatcgcgtgg
    23581 caggtgatct aatttggcac gtaaattcag gtaagccagc atgatgaata acggcggaag
    23641 catggacgct gcggcggtca tattaataat ggtattcatc agatcctgca cggtattgga
    23701 accgagcatc gggataatca tcagcgggat gacgatcagg aactggatcc acgctgcgcg
    23761 cgccggaacg ccgttttcgt tcagttcgac ggttttctta ccaaagatgc cttccggaat
    23821 ttcggagaag aaaattttca ccggcgttgc cgtccacatc agcagggaac cgaacatcgc
    23881 ggtaaaggac accagaccga caaagcgatt catcagtgct tccggtagac caaaatacgc
    23941 cgccatgccg tggaatacct gtaccgatcc gccagtaaat ttcaactctt tgctgctgac
    24001 gaagacgtta atcagcaccg aggagacgga atacagtacg ccgataaaaa tcccggcgag
    24061 gatgatcact ttaacgaacg atttcgaacc gcctttgacg tcgttaacgt acaccgcgac
    24121 ggactccgcg ccacctgcgg ccataaagat ccaggtggtc acgccgagga acgcccagtt
    24181 gaagttcggg atcatcgcgt caacggtgat cggatcggca ggctgtacgc cgccaaccag
    24241 tgccgtacct gccagtaaaa tgtaggagag tgttaacagc agcatcagcg ttgaagtgac
    24301 ggaggtgatt ggccccagca ttttcgcccc gttggtggaa acccatgtgg agaaggcgaa
    24361 cagcaccata ctgataatgg tggtggcaac cggcgtcata atatattcgt agccgaggaa
    24421 ggcatacgaa gcataagcaa taacgcgcgg caacaacgag gtgaaaaaga acaggtttac
    24481 gaaccagtag gtataggcag taataaatgc ccaacgtccg cccagcgagc tttttaccca
    24541 cgcgtagaca ccggcttccg agtttttatt taacgaaaca aattctgcga tgatcagaca
    24601 gaagggaata aaataaaaaa ttgttgcgag gaaaaacatc ggtgccgagg ccagtccaag
    24661 ctcaatatta ttattgataa cgttgttaaa gctgtaaacg gcggcaaaag tcagcgagag
    24721 caagccgaat ttgccgattg tattacgttt ggtatcagac ataacacatc tccgaaaatg
    24781 agtagggtag cggtggcggc aaactccttt tgccgccgta gttgttattt gttatggaaa
    24841 taaccatctt cgatggtgac tttgagaacc acttttttga ccgcgttatt gcagataaac
    24901 cgatacgctt catggatatc gcaaataacg atttgccctt cgtggacctc tacggtttct
    24961 ccgcagcctt ttaaatattc acggtcagtt tcatcacgat aatattccac tacctgtaat
    25021 gtctctttcg gcgcatattc aattttttgc tgcccttgca ggtagtaatg cacttcaaaa
    25081 tagcgtcgat gcccggtaaa tagcgcatcg gtcgcggagt ccgtctccac gcggtaagtc
    25141 aatgagtcac cgatggagtg ggcgacgcca ggctgaatgt tgtcgatatt ttcaatcgct
    25201 tcaacgcatc gttgccactt cttgccagag gcgtaaatct ggcggaactg ttctaagtta
    25261 tcgatgatcc tcattgctta ttctcgctgt gcaaattcgt ggaaaagaac cctgcgccga
    25321 actcatacga cgccaggctt tgcgcggtag cttctccgcc agaaaccggc agcaacgtaa
    25381 agccgtagct gaagtcacgg aaccagacgc gccaggagtc cagcacctcg ctgccccagg
    25441 agttggagcc gaggccaagc agctggtggt cgagattcag agtgatgtca tcactgcgct
    25501 gaagctcgtt acagtgctgg gcagcgtgga tgttttcctg ggtatagcgc caggcgctga
    25561 agttaatggg gcgctgcgga accaccagca gaccgttgcc gtggcgatta gtcagtgccg
    25621 tccagcggac atgctgacgg ttgccgttgt tctgcgggaa gggatagttc tcgaacatgg
    25681 catcgacggt actgcgccag atatcaatga tgttagcctg ctggctgtcg gcgtagtttt
    25741 cgcccggtcc acgtccgtaa tatgctacct gatcgtattc gccgttaatt cccatggtga
    25801 aaccgatgca cggaatgatg tgcggatagt cgccgtaacg ttcgccggaa agcgccacgt
    25861 taacctggcc atcggcagcg atgcgccaga tgtaggtgca gcgcatcccg aagtcaaaca
    25921 ccggtggggc aataaccgtg cggctgacta gcaacacttc atcatcactt tgctcaacga
    25981 caaaatcgcg cagatgctcc tgcatgatct gcaaatgatt tggctgccac agcccttcgt
    26041 actcctgctt atggttgtcg atcatcggct tgaagaagtt gatctttggc tcgcgggtca
    26101 gcagcgactc gccattcacc tgccaggatg tcggtttgcc actcattttt gagaaggtga
    26161 tcgcgaagtt gtagccgcga acggtgcagc tcaaacgatc gtcttccagc gtcagcggac
    26221 gcgcattatt tggcgcgaaa ggtacaggct gcgcggtgtt ttccttcagc gggaactgat
    26281 aagtggcgat agaatgcccg gcttcgctgt agcgggtgcg ggaatctttg gtcactgtaa
    26341 tgttgaggaa cgcttcgcgg gcgtccagct gcggcagcgt gatctgtaag ggggcttcgc
    26401 tgttcggcgc aacgtcgcgc agtttaatct gttgcgtcgc gagtgtttca ccttcggcgc
    26461 gcacctctgc gtgcagggtg tagtcatcta gcgtggtaaa ccacagttta ttttcgactt
    26521 tcaactcgcc gcgagtcaga tccagcgcgt ggatttttac tggcgcgata acctgtttgt
    26581 actctttcag gcccggtccc ggcgtctgat cggaatagat caaaccatca agacagaagt
    26641 tatagttgtt gggatagtcg ccgtagtcgc cgccgaattt ataccagaca ttgccgttgt
    26701 catcctgcgc ctggatcccg tggtcgcacc attcccagac ataatgtccc tgaatgcaat
    26761 catgcttata gaagacgttc tggtactccg tcagcccgcc cggtccgttc cccatcgcat
    26821 gagcatattc acagatgatg cgcggcttcg gatgcgggta ttcaccaaac tcattcatca
    26881 gcggcacgcg ggtgtacatg gtggaaataa tatcgaccac ttcagcatcg cgatcttctt
    26941 cgtaatgcac cagtcgcgtg tcatccagcg ccttcgccgc atggtacatc gcgcggatgt
    27001 tacagccata gccggattca ttgcccagcg accagatgat gatcgacgga tggtttttct
    27061 gcgcgtgaat atggcgaaca atgcgctcga cgtaaacttt ttcccactgc ggatcgtcgg
    27121 taatacggct gatatcgccg acattagcaa agccgtgcga ttcgacgtcg gtttccgcca
    27181 tcacaaacag gccgtagata tcgcacagtt cgtaaaaacg tggatcgttc gggtagtgag
    27241 cggtacgcac ggagttgatg ttgtgctgct tcatcaactg gagatctttc tcgacgcgat
    27301 ccatcccaac ggcgcggcct ttgcgatgat cgttgtcgtg gcggttgacg ccgtgcagca
    27361 tcacataacg gttattgatc cagaacagac cgtcgcgcac tttgatatca cggaagccaa
    27421 cgcgttgtgg caccacttcc agaacgttgc cgtcggcgtc tttcagcgtc atgaccagat
    27481 ggtaaagata aggggattct gctgaccatt gctgcggctg ttcgacagta aaagcaaaac
    27541 tggcgctggt cagtttttca attgccaaat gatcaatggc gctgctgtgc accacgtgtt
    27601 cgccatcaaa cagggtatat tccagcgtcg tgacgacagg ggaggcggcg agattttcca
    27661 gcaccacttc gcaggaaagt gtggcatcgc aataggcttc gtcaaagtcg gtacgaacgg
    27721 tgaaatcgtt aatatgcgtg agttgttttc cgatcagata aacatcgcgg aaaatccccg
    27781 ccgaccacca catatcctgg tcttccacgt aggtagagtc cgcccattgc atcacgcgca
    27841 cacacaacag gttgtcgccg gttttaacca tcgtgctgat gtcaaactct gcggtcaggc
    27901 gactgccctt gctgaacccc acatactgac cgttaacgta gacttcaaaa taggtttcga
    27961 cgccgtcaaa tttaatcagc gtctgtttac cctgccagcc gtcgctgagg gtgaaaatac
    28021 gttgataggc accggttggg ttatcgctgg ggacaaacgg cacatcgatg gggaacggaa
    28081 aaccttcgtc ggtatattgc agtttgccgt gaccttccat ttgccacatg gcggggacgg
    28141 taatatgccc ccagtcagcc attaactcag aggtgaaggc ttctggcact tgcagcggat
    28201 ggtcaaaaaa gtggaaattc cactgaccgc ttaagggcag aaacaggctg ctggtttcac
    28261 gggcaaaagt acgagcctgc gcaacagaat catatgaaaa aaagtacgca cgcggcgcaa
    28321 gtcggttttc gtgggtgagc tgaatgtttt cccagcgatt catagggcct cccggtagag
    28381 agaaatcgga accatttatg gtgatggtag tttgtgtaat tgctggggag tttagtaaaa
    28441 attttactaa aaagtagcgg ataaatgcat ttactttaac tggatcacga aaataaacgg
    28501 cagcactgtc cggcaaaaac gattgccgga cagcgtgcaa ggggatttaa cgggtcgtgc
    28561 cgcgcagttt taatttgctg ggaacgaaga ccaacagcgg cagcgcgcga ccatcgcggg
    28621 ctttttcata caccaggtta acgccctgac ttcccatcat ctcggaatgg atgcgcacgg
    28681 tggagagcgg cggaaaggta aatcgcgcgg tggggatatc gttaacgctg ataagcgaaa
    28741 tatcctgtgg gatgttcagg ccacgttcat gaattgcccg cagtacgccg atagcaatgg
    28801 aatcggaagc aacaaacagt gccttcggat agtcttcccg cgccagcatc tgttttgcca
    28861 gttcatagcc cgacgaactg gaaaaaccgc cgcgccagat atcctcttcg cgtaccacgt
    28921 gtttcagacg accatattcc gcaaaggcga cctcacgaat atccgccttg ccaggcaagt
    28981 cttcaccgcc aataaaacca atacgattaa cgccctgatt gatatagaaa tcgatgattt
    29041 ctttactgat acgtgccaga tcgatatcca ccgcatcgta accgctgccg ggttcgtgaa
    29101 agtcgataaa acagatattg tcggtcaacg cacaggcggc ggcgcgcagg gcggacgtgg
    29161 gtttgccgac aattaaaata ccggtgacgt ttttaatgtc tggtaagccg ttgtgttcat
    29221 aacagttggt cagttcgatc gccagctttt cgcactgagt ttcaatgccg tggcggatcg
    29281 ccagatagta aggatcgttg atctccagct cctgttggta gctgtagata gccagaatat
    29341 ggtgttggtt gactgcgcct gtctggagtt tacgggcgct actggtcttg tactccagct
    29401 tttcggcgat ctcgagaatg cgatgtttcg tctcttcttt cacattcaat gtcggatcgt
    29461 cattcaagac cctggatact gtcgccaggg atacgccagc ttcgattgcg atgtctttta
    29521 gtgttgccat gtttaccttt tttactttcc ctgatgtgcg cccccattgt agaggaagtg
    29581 cttatcgggt cacctgtttt cagtaaataa agcgcgtttc tttgacgctg taacggtaac
    29641 acagcggctt gcgaatgtga tcacgcctgc attctccatt tttagcctac aggattgaga
    29701 aaaacttttg ctatagttta gtaaaatttt acttgaaggg aggaaacaat ggaaaccgtg
    29761 gcttacgctg attttgcacg tctggaaatg cgcgtcggaa agattgtgga agtgaaacgc
    29821 catgaaaacg ccgacaagct gtacatcgta caggttgatg tgggggaaaa aacgctgcaa
    29881 accgtgacca gtctggtgcc gtactacagc gaagaagagc tgatgggaaa aacagtggtg
    29941 gtcttatgca atctgcaaaa agcgaaaatg cgcggtgaaa cgtcggaatg catgctgttg
    30001 tgtgcggaaa ccgatgacgg cagtgaaagc gtgctgttaa cgccggagcg gatgatgcct
    30061 gcgggcgtac gtatcgtata aaaagatcgg atggcgacgt cgtatcgcca tccgatttga
    30121 tattacgctt cttcgacact gactcgcatg gccgccagcg ccttacgcgc cgctttgatc
    30181 accagttcac actgttcaat ggtcagtgtc agtggcggtt caatgcggat cgttttagcg
    30241 ttgttgagtg ttccggccac cagtacgcgc tggcggaaca tctcgctggc aaagttatag
    30301 ccgatttcgt tatcaacaaa ctcaatcgcc atcaacatcc ctttgccacg cgcttcctgt
    30361 accagatcgg gatattcccg cgccagttga cggaaaccgt ccagcaacat atcgcctttt
    30421 tgttcagcct gagccggtaa gttctgctcc agcaacacat tgatggtcgc cagcgccgcc
    30481 gcacaggcca gcgggttgcc gccaaaggtg gtggtatgca ggaacggatt gtcgaacagc
    30541 acggagaaca cctcttcagt ggcgatagtt gcgccaatcg gcatcacgcc accgccgagc
    30601 gctttggcaa ggcagaggat atccggctgt acgttctcat gctcgcaggc gaacatcttg
    30661 cccgtgcgcc ccatgcccgt ttgtacttca tcgaggatca tcagtgcgcc gaactcatcg
    30721 cacagcttac gcacggcggt gagatagcct ggcggcggca gaattacgcc accttcgccc
    30781 tgaatcggtt cgaggatcac cgcagcaaca tcatcaccgg tttttttgca ctcgttaaga
    30841 gccgtgcgca tggcttcgat attgccaaac ggtacatgac gaaaccctgg cagcagtggc
    30901 ataaaaggtt tgcggaaggt cgatttcgcc gtggccgaca gcgcgccaag tgatttaccg
    30961 tggaacgcgc cgctggtggc aataaaagta aacttgccgc gcggtgactg ataagccttc
    31021 gccagcttca gcgccgcttc aacggattcg gtgccgctat tacagaagaa gctgtatttc
    31081 agtttaccgg gcgttagcgc cgcaagggtt ttcgccagca tcgcccgtaa cggatcgagc
    31141 agctcctggc tgtgaagcgg ttgtttagca agttgattct gtacggcgga aaccacaact
    31201 ggattacggt gccccacgtt gaaaattcca aaacctccca ggcagtcgat aaactcctgt
    31261 ccctgggtgt cgacaagcgt atttaaacct cccgcttgcc actctacggc tccgtaatcc
    31321 ccgccggcgg taacagattt gcgatactct aaaaaccccg gattgacatg ctctttgaag
    31381 tattcaatca cctctcggtt aagtgctttc atctcctcat gatccagcgt tcgcttctca
    31441 atgagattca gggcgtgggc gctgcacgct aaagccgatg cgctcgaagg taacctgttc
    31501 aaaatatgct ccggaggctc gcgtatcaca tgataccgat ttaagtattg cagggattgc
    31561 gccactccgg ctgcgatcgc gaaaatcggg ataaacacaa atgtggttat catttgcgca
    31621 atatttaatg ctatttgtta acatctgttg ttattttgca cggtttttgt cgggtatgga
    31681 tttggcgatt gctgcgcaaa tgcacacttt cagggcaaca actgcctgat ttttgggcaa
    31741 gataattaac gttttcgtgt agtaacagag tgaaagtatt ttaaatcaag ggattagttt
    31801 atgctttaat cgcaaattgc gatctaaatc aaattaatca gttaaagata accgcagcgg
    31861 ggccgacata aactctgaca agaagttaac aaccatataa cctgcacagg acgcgaacat
    31921 gtcttctcat ccgtatgtca cccagcaaaa taccccgctg gcggacgata ccactctgat
    31981 gtccactacc gatctgcaaa gctatatcac tcatgctaat gacacttttg tgcaggtgag
    32041 cggctatacc ttgcaagagt tacaagggca gccgcacaac atggtgcgtc acccggatat
    32101 gccaaaagcg gcatttgcgg atatgtggtt caccctgaaa aaaggggagc cctggagtgg
    32161 catcgtgaaa aatcgccgca aaaatggcga ccattattgg gtgcgggcca atgcggtacc
    32221 gatggtgcgc gagggaaaaa tcagtggcta tatgtcgatt cgtacccggg cgacggatga
    32281 agagatcgcg tcggtggagc cgctgtacaa agcgctgaac gccggacgta ccggtaagcg
    32341 tattcataaa ggcctggtgg tgcgtaaagg ctggctgggt aaactgcctt cattaccgct
    32401 tcgctggcgg gtgcgtggag tgatgaccct gatgtttatc ttgctggcgg ccatgctctg
    32461 gtttgtcgct gccccggtgg tgacgtattt cctctgtgcg ttagtgatgt tgttgggaaa
    32521 tgcctgtttt gaatggcaaa ttgtacgtcc gatagaaaat gtcgccagcc aggcactgaa
    32581 agtggcgacc ggagaacgta atagtgttga gcatctgaag cgcagcgatg agctggggct
    32641 gacattacgc gcggtagggc agcttggcct gatgtgccgt tggctaatta atgatgtctc
    32701 aagccaggtg tccagtgtca gaaacggcag tgagacgctg gcgaaaggca ccgatgaact
    32761 gaacgaacat acccagcaga cagttgataa cgttcagcaa acggtggcga ccatgaacca
    32821 aatggcggcg tcggtgaaac agaactctgc cacggcgtcg gctgccgata aactttctat
    32881 caccgccagt aatgcggcag tgcagggcgg ggaggcgatg actacggtga tcaagacaat
    32941 ggacgatatt gccgacagta cccagcgcat tggcaccatt acttcgctga ttaacgatat
    33001 tgcgtttcag accaatattc tggccctgaa tgcggcggtg gaagcggcgc gtgccggcga
    33061 acagggcaaa ggttttgcag tggtggcggg ggaagtgcgt catttagcca gccgcagcgc
    33121 caatgctgcc aacgatattc gcaagctgat tgatgccagt gctgataagg tgcaatctgg
    33181 ttcgcagcag gtacacgccg ccggacggac gatggaagat attgtggcgc aggtaaaaaa
    33241 cgtcacccag ttgatagctc agattagcca ttcaacgctg gaacaggccg atgggctttc
    33301 cagcctgacc cgtgcagtgg atgagcttaa tctcatcacc cagaaaaacg ccgagttagt
    33361 ggaagagagt gcgcaggtgt cggcgatggt gaaacaccgc gccagccgac tggaagacgc
    33421 ggtgacggtg ctgcattaat cggtatgccg gatccggcaa tcggtgatta atccagttgc
    33481 gtaatatcaa aagcggcgcg gtcgatcacc gcaatgatct tttttatttg tgcatcagtg
    33541 atatcgctct ggttgacgcg taaatccagc accgctttaa aattatccag cgcccgcttc
    33601 atttgcgggt tctggcgcag cgcagcgcca acgcaacgcg ctttgatgcg ttcttcaatc
    33661 atctctacct gttcgcggtt ttcttccagc cactgcgcgc cttgttcggt cagcgcaatc
    33721 tgcttcttac caccttcctc ttcgcggatg gtaatcagcg actgctcctg cagaaaatcc
    33781 agcgtcgggt agatgacgcc cgggcttggg gtgtaattcc cctgggtcag attttcaatc
    33841 gctttaatca actcgtaacc gtggctgtca tcgcgcgaga gaatatccag aatcaccaga
    33901 cgcaattcac cgtgaccaaa gaaacgttgt cgacgaccgc cgccgcgacc gtggcgacca
    33961 ccgcagcatt gaccgtgttc atgctggtga ccgtgtccgc agtgctcgtg ttctgacttc
    34021 tcacctttgc agcagccctc atggcgtggc tggccttcat gtttacaaca cccttcgtga
    34081 tgatggctca tat
//
LOCUS       DADSYM010000035        33921 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2415
            SAMN08039579-rid11723173.denovo.035, whole genome shotgun sequence.
ACCESSION   DADSYM010000035 DADSYM010000000
VERSION     DADSYM010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039579
            Sequence Read Archive: SRR6362533
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 33921)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33921)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980162.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 03:39:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,484
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 4,347
            CDSs (with protein)               :: 4,347
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 43 of 85
            Pseudo Genes (incomplete)         :: 37 of 85
            Pseudo Genes (internal stop)      :: 19 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33921
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039579-rid11723173.denovo.035"
                     /strain="LGE2415"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8302 N 95.9628 W"
                     /collection_date="2015-08-24"
     gene            complement(142..354)
                     /gene="cspH"
                     /locus_tag="J1Y83_001404"
     CDS             complement(142..354)
                     /gene="cspH"
                     /locus_tag="J1Y83_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock-like protein CspH"
                     /protein_id="HBA7629563.1"
                     /translation="MSRKMTGIVKTFDRKSGKGFIIPSDGRKEVQVHISAFTPRDAEV
                     LIPGLRVEFCRVNGLRGPTAANVYLS"
     gene            640..852
                     /gene="cspG"
                     /locus_tag="J1Y83_001405"
     CDS             640..852
                     /gene="cspG"
                     /locus_tag="J1Y83_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock protein CspG"
                     /protein_id="HBA7629564.1"
                     /translation="MSNKMTGLVKWFNADKGFGFITPDDGSKDVFVHFTAIQSNEFRT
                     LNENQKVEFSIEQGQRGPAAANVVTL"
     gene            863..1051
                     /locus_tag="J1Y83_001406"
     CDS             863..1051
                     /locus_tag="J1Y83_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HBA7629565.1"
                     /translation="MTQHLHFRCPCCHGSQYRTSAFDVTERNPLGAKCIFCKSTMITF
                     DNVALQIRTDHAPLDFTK"
     gene            1026..1256
                     /gene="ymcE"
                     /locus_tag="J1Y83_001407"
     CDS             1026..1256
                     /gene="ymcE"
                     /locus_tag="J1Y83_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmcE"
                     /protein_id="HBA7629566.1"
                     /translation="MRRWISQNNIRLPRGAFFISALFFFNAVRIVSDNLLIIESFGEM
                     AYNISYLTRVPGTNTLLACCCLLRPEEVNSEY"
     gene            1246..1419
                     /locus_tag="J1Y83_001408"
     CDS             1246..1419
                     /locus_tag="J1Y83_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module toxin, GnsA/GnsB family"
                     /protein_id="HBA7629567.1"
                     /translation="MNIEELKKQAETEIADFIAQKIAELNKNTGKEVSEIRFTAREKM
                     TGLESYDVKIKIM"
     gene            complement(1468..2541)
                     /locus_tag="J1Y83_001409"
     CDS             complement(1468..2541)
                     /locus_tag="J1Y83_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HBA7629568.1"
                     /translation="MAEKKRTRWQRRPGTTGGKLPWNDWRNATTWRKATQLLLLAINI
                     YIAITFWYWVRYYETAGSTTFVARPGGIEGWLPIAGLMNLKYSLATGQLPSVHAAAML
                     LLVSFIVISLLLKKAFCSWLCPVGTLSELIGDLGNKLFGRQFVLPRWLDIPLRGVKYL
                     LLSFFLYIALLMPAQAIHYFMLSPYSVVMDVKMLDFFRHMGTATLISMIVLLIASLFI
                     RHAWCRYLCPYGALMGVVSLLSPFKIRRNAESCIDCGKCAKSCPSRIPVDKLIQVRTV
                     ECTGCMTCVESCPVASTLTFSLQKPAANKKAFALSGWLMTLLVMGIMFAAIGYAIYAG
                     VWQSPVPEELYQRLIPQSPMIGH"
     gene            complement(2624..5356)
                     /gene="torS"
                     /locus_tag="J1Y83_001410"
     CDS             complement(2624..5356)
                     /gene="torS"
                     /locus_tag="J1Y83_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001054736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TMAO reductase system sensor histidine
                     kinase/response regulator TorS"
                     /protein_id="HBA7629569.1"
                     /translation="MNLTLTRRLWMGFALMALLTLTSTLVGWYNLRFISQVEKDNTQA
                     LIPTMNMARQLSEASAWELFAAQNLTSADNEKMWQAQGRMLTAQSLKINALLQALREQ
                     GFDTTAIEQQEQEISRSLRQQGELVGQRLQLRQQQQQLSQQIVAAADEIARLAQGQAN
                     NAATSAGATQAGIYDLIEQHQRQAAESALDRLIDIDLEYVNQMNELRLSALRVQQMVM
                     NLGLEQIQKNAPTLEKQLNNAVKILQRRQIRIEDPGVRTQVATTLTTVSQYSDLLALY
                     QQDSEISNHLQTLAQNNIAQFAQFSSEVSQLVDTIELRNQHGLAHLEKASARGQYSLL
                     LLGIVSLCALILILWRVVYRSVTRPLAEQTQALQRLLDGDIDSPFPETAGVRELDTIG
                     RLMDAFRSSVHALNRHREQLAAQVKARTAELQELVIEHRQARAEAEKASQAKSAFLAA
                     MSHEIRTPLYGILGTAQLLADNPALNAQRDDLRAITDSGESLLTILNDILDYSAIEAG
                     GKNVSVSDEPFEPRPLLESTLQLMSGRVKGRPIRLATEIADDVPTALMGDPRRIRQVI
                     TNLLSNALRFTDEGHIILRSRTDGEQWLVEVEDSGCGIDPAKLAEIFQPFVQVSSKRG
                     GTGLGLTISSRLAQAMGGELSATSTPEVGSCFCLRLPLRIATAPVPKTVNQAVRLDDL
                     RLLLIEDNPLTQRITVEMLNTSGAQVVAVGNAAQAIEALQNSEPFAAALVDFDLPDID
                     GITLARQLAQQYPSLVLIGFSAHVIDETLRQRTSSLFRGIIPKPVPREVLGQLLAHYL
                     QLQVNNDQPLDVSQLNEDAHLMGAEKIHEWLILFKQHALPLLNEIDIARASQDNEKIK
                     RAAHQLKSSCSSLGMRSASQQCAQLEQQPLSAPLPHEEITRSVAALEAWLIRKT"
     gene            5439..6467
                     /gene="torT"
                     /locus_tag="J1Y83_001411"
     CDS             5439..6467
                     /gene="torT"
                     /locus_tag="J1Y83_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TMAO reductase system periplasmic protein TorT"
                     /protein_id="HBA7629570.1"
                     /translation="MRVLLLLLLSLFMLSAFSADNLLRWHDAQHYTVQASTPLKAKRA
                     WKLCALYPSLKDSYWLSLNYGMQEAARRYGVDLKVLEAGGYSQLATQQAQIDQCKQWG
                     AEAILLGSSTTSFPDLQKQVANLPVIELVNAIDAPQVKSRVGVPWFQMGYQPGRYLVQ
                     WAHGKPLNVLLMPGPDNAGGSKEMVEGFRAAIAGSPVRIVDIALGDNDIEIQRNLLQE
                     MLERHPEIDVVAGTAIAAEAAMGEGRNLKTPLTVVSFYLSHQVYRGLKRGRVIMAASD
                     QMVWQGELAVEQAIRQLQGQSVSDNVSPPILVMTPKNADREHIRRSLSPGGFRPVYFY
                     QHTSAAKK"
     gene            complement(6440..7132)
                     /gene="torR"
                     /locus_tag="J1Y83_001412"
     CDS             complement(6440..7132)
                     /gene="torR"
                     /locus_tag="J1Y83_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator TorR"
                     /protein_id="HBA7629571.1"
                     /translation="MPHHIVIVEDEPVTQARLQSYFTQEGYTVSVTASGAGLREIMQN
                     QPVDLILLDINLPDENGLMLTRALRERSTVGIILVTGRSDRIDRIVGLEMGADDYVTK
                     PLELRELVVRVKNLLWRIDLARQAQPHTQDNCYRFAGYCLNVSRHTLERDGEPIKLTR
                     AEYEMLVAFVTNPGEILSRERLLRMLSARRVENPDLRTVDVLIRRLRHKLSADLLVTQ
                     HGEGYFLAADVC"
     gene            7262..8434
                     /gene="torC"
                     /locus_tag="J1Y83_001413"
     CDS             7262..8434
                     /gene="torC"
                     /locus_tag="J1Y83_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentaheme c-type cytochrome TorC"
                     /protein_id="HBA7629572.1"
                     /translation="MRKLWNALRRPSARWSVLALVATGIVIGIALIVLPHVGIKVTST
                     TEFCVSCHSMQPVYEEYKQSVHFQNASGVRAECHDCHIPPDIPGMVKRKLEASNDIYQ
                     TFIAHSIDTPEKFEAKRAELAEREWARMKENNSATCRSCHNYDAMDHAKQHPEAARQM
                     KVAAKDNQSCIDCHKGIAHQLPDMSSGFRKQFDELRASANDSGDTLYSIDIKPIYAAK
                     GDKEASGSLLPASEVKVLKRDGDWLQIEITGWTESAGRQRVLTQFPGKRIFVASIRGD
                     VQQQVKTLEKTTVADTNTEWSKLQATAWMKKGDMVNDIKPIWAYADSLYNGTCNQCHG
                     APEISHFDANGWIGTLNGMIGFTSLDKREERTLLKYLQMNASDTAGKAHGDKKEEK"
     gene            8434..10980
                     /gene="torA"
                     /locus_tag="J1Y83_001414"
     CDS             8434..10980
                     /gene="torA"
                     /locus_tag="J1Y83_001414"
                     /EC_number="1.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine-N-oxide reductase TorA"
                     /protein_id="HBA7629573.1"
                     /translation="MNNNDLFQASRRRFLAQLGGLTVAGMLGPSLLTPRRATAAQAAT
                     EAVISKEGILTGSHWGAIRATVKDGRFVAAKPFELDKYPSKMIAGLPDHVHNAARIRY
                     PMVRVDWLRKRHLSDTSQRGDNRFVRVSWDEALDMFYEELERVQKTHGPSALLTASGW
                     QSTGMFHNASGMLAKAIALHGNSVGTGGDYSTGAAQVILPRVVGSMEVYEQQTSWPLV
                     LQNSKTIVLWGSDLLKNQQANWWCPDHDVYEYYEQLKAKVAAGEIEVISIDPVVTSTH
                     EYLGREHVKHIAVNPQTDVPLQLALAHTLYSENLYDKNFLANYCVGFEQFLPYLLGEK
                     DGQPKDAAWAEKLTGIDAETIRGLARQMAANRTQIIAGWCVQRMQHGEQWAWMIVVLA
                     AMLGQIGLPGGGFGFGWHYNGAGTPGRKGVILSGFSGSTSIPPVHDNSDYKGYSSTIP
                     IARFIDAILEPGKVINWNGKSVKLPPLKMCIFAGTNPFHRHQQINRIIEGWRKLETVI
                     AIDNQWTSTCRFADIVLPATTQFERNDLDQYGNHSNRGIIAMKQVVPPQFEARNDFDI
                     FRELCRRFNREEAFTEGLDEMGWLKRIWQEGVQQGKGRGVHLPAFDDFWNNKEYVEFD
                     HPQMFVRHQAFREDPDLEPLGTPSGLIEIYSKTIADMNYDDCQGHPMWFEKIERSHGG
                     PGSQKYPLHLQSVHPDFRLHSQLCESETLRQQYTVAGKEPVFISPQDASARGIRHGDV
                     VRVFNVRGQVLAGAVVSDRYAPGVARIHEGAWYDPDKGGEPGALCKYGNPNVLTIDIG
                     TSQLAQATSAHTTLVEIEKYNGTVEQVTAFNGPVEMVAQCEYVPASQVKS"
     gene            10977..11576
                     /gene="torD"
                     /locus_tag="J1Y83_001415"
     CDS             10977..11576
                     /gene="torD"
                     /locus_tag="J1Y83_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000209906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone TorD"
                     /protein_id="HBA7629574.1"
                     /translation="MTTLTAQQIACVYAWLAQLFSRELDDEQLTQIASAQMAEWFSLL
                     KSEPPLTAAVNGLENSIATLTVRDDARLELAADFCGLFLMTDKQAALPYASAYKQDEQ
                     EIKRLLVEAGMETSGNFNEPADHLAIYLELLSHLHFSLGEGTVPARRIDGLRQKTLTA
                     LRQWLPEFVARCHQYDRFGFYAALSQLLQVLVECDNQKG"
     gene            complement(11832..12137)
                     /gene="cbpM"
                     /locus_tag="J1Y83_001416"
     CDS             complement(11832..12137)
                     /gene="cbpM"
                     /locus_tag="J1Y83_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone modulator CbpM"
                     /protein_id="HBA7629575.1"
                     /translation="MANVTVTFTITEFCLHTGISEEELNEIVGLGVVEPSEIQETTWV
                     FDDHAAIVVQRAVRLRHELALDWPGIAVALTLMDDIAHLKQENRLLRQRLSRFVAHP"
     gene            complement(12137..13057)
                     /gene="cbpA"
                     /locus_tag="J1Y83_001417"
     CDS             complement(12137..13057)
                     /gene="cbpA"
                     /locus_tag="J1Y83_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curved DNA-binding protein"
                     /protein_id="HBA7629576.1"
                     /translation="MELKDYYAIMGVKPTDDLKTIKTAYRRLARKYHPDVSKEPDAEA
                     RFKEVAEAWEVLSDEQRRAEYDQMWQHRNDPQFNRQFHHGDGQSFNAEDFDDIFSSIF
                     GQHARQSRQRPATRGHDIEIEVAVFLEETLTEHKRTISYNLPVYNAFGMIEQEIPKTL
                     NVKIPAGVGNGQRIRLKGQGTPGENGGPNGDLWLVIHIAPHPLFDIVGQDLEIVVPVS
                     PWEAALGTKVTVPTLKESILLTIPPGSQAGQRLRVKGKGLVSKKQTGDLYAVLKIVMP
                     PKPDENTAALWQQLADAQSSFDPRKDWGKA"
     gene            13592..14833
                     /gene="agp"
                     /locus_tag="J1Y83_001418"
     CDS             13592..14833
                     /gene="agp"
                     /locus_tag="J1Y83_001418"
                     /EC_number="3.1.3.10"
                     /EC_number="3.1.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glucose-1-phosphatase/inositol
                     phosphatase"
                     /protein_id="HBA7629577.1"
                     /translation="MNKTLIAATVAGIVLLASNAQAQTVPEGYQLQQVLMMSRHNLRA
                     PLANNGSVLEQSTPNKWPEWDVPGGQLTTKGGVLEVYMGHYMREWLAEQGMVKSGECP
                     PPDTVYAYANSLQRTVATAQFFITGAFPGCDIPVHHQEKMGTMDPTFNPVITDDSAAF
                     SEQAVAAMEKELSKLQLTDSYKLLEQIVNYKDSPACKEKQQCSLVDGKNTFSAKYQQE
                     PGVSGPLKVGNSLVDAFTLQYYEGFPMDQVAWGEIKSDQQWKVLSKLKNGYQDSLFTS
                     PEVARNVAKPLVSYIDKALVTDRTSAPKITVLVGHDSNIASLLTALDFKPYQLHDQNE
                     RTPIGGKIVFQRWHDSKANRDLMKIEYVYQSAEQLRNADALTLQAPAQRVTLELSGCP
                     IDANGFCPMDKFDRVLNEAVK"
     gene            complement(14871..15098)
                     /locus_tag="J1Y83_001419"
     CDS             complement(14871..15098)
                     /locus_tag="J1Y83_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002898701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA7629578.1"
                     /translation="MPTQEAKAHHVGEWASLRNTSPEIAEAIFEVAGYDEKMAEKIWE
                     EGSDEVLVKAFAKTDKDSLFWGEQTIERKNV"
     gene            complement(15119..15715)
                     /gene="wrbA"
                     /locus_tag="J1Y83_001420"
     CDS             complement(15119..15715)
                     /gene="wrbA"
                     /locus_tag="J1Y83_001420"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HBA7629579.1"
                     /translation="MAKVLVLYYSMYGHIETMARAVAEGASKVDGAEVVVKRVPETMP
                     PQLFEKAGGKTQTAPVATPQELADYDAIIFGTPTRFGNMSGQMRTFLDQTGGLWASGA
                     LYGKLASVFSSTGTGGGQEQTITSTWTTLAHHGMVIVPIGYAAQELFDVSQVRGGTPY
                     GATTIAGGDGSRQPSQEELSIARYQGEYVAGLAVKLNG"
     gene            16088..16261
                     /locus_tag="J1Y83_001421"
     CDS             16088..16261
                     /locus_tag="J1Y83_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="general stress protein"
                     /protein_id="HBA7629580.1"
                     /translation="MANHRGGSGNFAEDRERASEAGKKGGQHSGGNFKNDPQRASEAG
                     KKGGKSSHGKSDN"
     gene            complement(16344..17672)
                     /gene="rutG"
                     /locus_tag="J1Y83_001422"
     CDS             complement(16344..17672)
                     /gene="rutG"
                     /locus_tag="J1Y83_001422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415526.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization transport protein G"
                     /protein_id="HBA7629581.1"
                     /translation="MAMFGFPHWQLKSTSTESGVVAPDERLPFAQTAVMGVQHAVAMF
                     GATVLMPILMGLDPNLSILMSGIGTLLFFFITGGRVPSYLGSSAAFVGVVIAATGFNG
                     QGINPNIGIALGGIIACGLVYTVIGLVVMKIGTRWIERLMPPVVTGAVVMAIGLNLAP
                     IAVKNVSASAFDSWMAVMTVLCIGLVAVFTRGMIQRLLILVGLIVACLLYGVMTNVLG
                     LGKAVDFTLVSHAAWFGLPHFSTPAFNGQAMMLIAPVAVILVAENLGHLKAVAGMTGR
                     NMDPYMGRAFVGDGLATMLSGSVGGSGVTTYAENIGVMAVTKVYSTLVFVAAAVIAML
                     LGFSPKFGALIHTIPAAVIGGASIVVFGLIAVAGARIWVQNRVDLSQNGNLIMVAVTL
                     VLGAGDFALTLGGFTLGGIGTATFGAILLNALLSRRLVDVPPPEVVHQKP"
     gene            complement(17693..18187)
                     /gene="rutF"
                     /locus_tag="J1Y83_001423"
     CDS             complement(17693..18187)
                     /gene="rutF"
                     /locus_tag="J1Y83_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309280.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization flavin reductase protein
                     F"
                     /protein_id="HBA7629582.1"
                     /translation="MNIVDQQTFRDAMSCMGAAVNIITTDGPAGRAGFTASAVCSVTD
                     TPPTLLVCLNRGASVWPVFNENRTLCVNTLSAGQESLSNLFGGKTPMEHRFAAARWQT
                     GVTGCPQLEEALVSFDCRISQVVSVGTHDILFCAIEAIHRHATPYGLVWFDRSYHALM
                     RPAC"
     gene            complement(18198..18788)
                     /locus_tag="J1Y83_001424"
     CDS             complement(18198..18788)
                     /locus_tag="J1Y83_001424"
                     /EC_number="1.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonic semialdehyde reductase"
                     /protein_id="HBA7629583.1"
                     /translation="MNEAVSPGALSTLFTDARTHNGWREPPVSDETLREIYAQMKWGP
                     TSANCSPARIVFIRTAEGKERLRPALSSGNLQKTLTAPVTAIVAWDSEFYERLPQLFP
                     HGDARSWFTSSPQLAEETAFRNSSMQAAYLIVACRALGLDTGPMSGFDRQYVDDAFFA
                     GSTLKSNLLINIGYGDSSKLFARLPRLSFEEACGLL"
     gene            complement(18798..19598)
                     /gene="rutD"
                     /locus_tag="J1Y83_001425"
     CDS             complement(18798..19598)
                     /gene="rutD"
                     /locus_tag="J1Y83_001425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization protein D"
                     /protein_id="HBA7629584.1"
                     /translation="MKLSLSPPPYADAPVVVLISGLGGSGSYWLPQLAVLEQEYQVVC
                     YDQRGTGNNPDTLAEDYSIAQMAAELHQALVAAGIERYAVIGHALGALVGMQLALDYP
                     ASVTVLVSVNGWLRINAHTRRCFQVREQLLHSGGAQAWVEAQPLFLYPADWMAARAPR
                     LEAEDALALAHFQGKNNLLRRLNALKRADFSRHADRIRCPVQIICASDDLLVPSACSS
                     ELHAALPDSQKMVMRYGGHACNVTDPETFNALLLNGLASLLHHREAAL"
     gene            complement(19606..19992)
                     /gene="rutC"
                     /locus_tag="J1Y83_001426"
     CDS             complement(19606..19992)
                     /gene="rutC"
                     /locus_tag="J1Y83_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization protein C"
                     /protein_id="HBA7629585.1"
                     /translation="MPKSVIIPAGSSAPLAPFVPGTLADGVVYVSGTLAFDQHNNVLF
                     ADDPKAQTRHVLETIRKVIETAGGTMADVTFNSIFITDWKNYAAINEIYAEFFPGDKP
                     ARFCIQCGLVKPDALVEIATIAHIAK"
     gene            complement(20004..20696)
                     /gene="rutB"
                     /locus_tag="J1Y83_001427"
     CDS             complement(20004..20696)
                     /gene="rutB"
                     /locus_tag="J1Y83_001427"
                     /EC_number="3.5.1.110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxyureidoacrylate/ureidoacrylate
                     amidohydrolase RutB"
                     /protein_id="HBA7629586.1"
                     /translation="MTTLTARPEAITFDPQQTALIVVDMQNAYATPGGYLDLAGFDVS
                     TTRPVIANIQTAVTAARTAGMLIIWFQNGWDEQYVEAGGPGSPNFHKSNALKTMRNQP
                     QLQGKLLAKGSWDYQLVDELVPQPGDIVLPKPRYSGFFNTPLDSILRSRGIRHLVFTG
                     IATNVCVESTLRDGFFLEYFGVVLEDATHQAGPEFAQKAALFNIETFFGWVSDVETFC
                     DALSSTSFARIA"
     gene            complement(20696..21787)
                     /gene="rutA"
                     /locus_tag="J1Y83_001428"
     CDS             complement(20696..21787)
                     /gene="rutA"
                     /locus_tag="J1Y83_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309285.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization protein A"
                     /protein_id="HBA7629587.1"
                     /translation="MKIGVFVPIGNNGWLISTHAPQYMPTFELNKAIVQKAEHYHFDF
                     ALSMIKLRGFGGKTEFWDHNLESFTLMAGLAAVTSRIQIYATAATLTLPPAIVARMAA
                     TIDSISGGRFGVNLVTGWQKPEYEQMGIWPGDDYFSRRYDYLTEYVQVLRDLWGTGKS
                     DFKGDFFTMNDCRVSPQPSVPMKVICAGQSDAGMAFSARYADFNFCFGKGVNTPTAFA
                     PTAARMKQAAEQTGRDVGSYVLFMVIADETDDAARAKWEHYKAGADEEALSWLTEQSQ
                     KDTRSGTDTNVRQMADPTSAVNINMGTLVGSYASVARMLDEVASVPGAEGVLLTFDDF
                     LSGIENFGERIQPLMQCRAHLPALTQEVA"
     gene            22075..22713
                     /gene="rutR"
                     /locus_tag="J1Y83_001429"
     CDS             22075..22713
                     /gene="rutR"
                     /locus_tag="J1Y83_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator RutR"
                     /protein_id="HBA7629588.1"
                     /translation="MTQGAVKTTGKRSRAVSAKKKAILSAALDTFSQFGFHGTRLEQI
                     AELAGVSKTNLLYYFPSKEALYIAVLRQILDIWLAPLKAFREDFAPLAAIKEYIRLKL
                     EVSRDYPQASRLFCMEMLAGAPLLMDELTGDLKALIDEKSALIAGWVKSGKLAPIDPQ
                     HLIFMIWASTQHYADFAPQVEAVTGATLRDEVFFNQTVENVQRIIIEGIRPR"
     gene            complement(22753..26715)
                     /gene="putA"
                     /locus_tag="J1Y83_001430"
     CDS             complement(22753..26715)
                     /gene="putA"
                     /locus_tag="J1Y83_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001462364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trifunctional transcriptional regulator/proline
                     dehydrogenase/L-glutamate gamma-semialdehyde
                     dehydrogenase"
                     /protein_id="HBA7629589.1"
                     /translation="MGTTTMGVKLDDATRERIKSAATRIDRTPHWLIKQAIFSYLEQL
                     ENSDTLPELPALLSGAANESDEAPTPAEEPHQPFLDFAEQILPQSVSRAAITAAYRRP
                     ETEAVSMLLEQARLPQPVAEQAHKLAYQLADKLRNQKNASGRAGMVQGLLQEFSLSSQ
                     EGVALMCLAEALLRIPDKATRDALIRDKISNGNWQSHIGRSPSLFVNAATWGLLFTGK
                     LVSTHNEASLSRSLNRIIGKSGEPLIRKGVDMAMRLMGEQFVTGETIAEALANARKLE
                     EKGFRYSYDMLGEAALTAADAQAYMVSYQQAIHAIGKASNGRGIYEGPGISIKLSALH
                     PRYSRAQYDRVMEELYPRLKSLTLLARQYDIGINIDAEEADRLEISLDLLEKLCFEPE
                     LAGWNGIGFVIQAYQKRCPLVIDYLIDLATRSRRRLMIRLVKGAYWDSEIKRAQMDGL
                     EGYPVYTRKVYTDVSYLACAKKLLAVPNLIYPQFATHNAHTLAAIYQLAGQNYYPGQY
                     EFQCLHGMGELLYEQVTGKVADGKLNRPCRIYAPVGTHETLLAYLVRRLLENGANTSF
                     VNRIADTSLPLDELVADPVTAVEKLAQQEGQTGLPHPKIPLPRDLYGHGRDNSAGLDL
                     ANEHRLASLSSALLNSALQKWQALPMLEQSVAAGEMSPVINPAEPKDIVGYVREATPR
                     EVEQALESAVNNAPIWFATPPAERAAILHRAAVLMESQMQQLIGILVREAGKTFSNAI
                     AEVREAVDFLHYYAGQVRDDFANETHRPLGPVVCISPWNFPLAIFTGQIAAALAAGNS
                     VLAKPAEQTPLIAAQGIAILLEAGVPPGVVQLLPGRGETVGAQLTGDERVRGVMFTGS
                     TEVATLLQRNIASRLDAQGRPIPLIAETGGMNAMIVDSSALTEQVVIDVLASAFDSAG
                     QRCSALRVLCLQDEIADHTLKMLRGAMAECRMGNPGRLTTDIGPVIDGEAKTNIERHI
                     QTMRSKGRPVFQAVRENSEDAREWQSGTFVAPTLIELDDFAELQKEVFGPVLHVVRYN
                     RNQLPALIEQINASGYGLTLGVHTRIDETIAQVTGSAHVGNLYVSRNMVGAVVGVQPF
                     GGEGLSGTGPKAGGPLYLYRLLANRPESALAVTLARQDAEYPVDAQLKAALTQPLNAL
                     REWAANRPELQALCTQYGELAQAGTQRLLPGPTGERNTWTLLPRERVLCIADDEQDAL
                     TQLAAVLAVGSQVLWPDDALHRQLVKALPSAVSERIQLAKAENITAQPFDAVIFHGDS
                     DQLRALCEAVAARDGAIVSVQGFARGESNILLERLYIERSLSVNTAAAGGNASLMTIG
                     "
     gene            26770..26979
                     /locus_tag="J1Y83_001431"
     CDS             26770..26979
                     /locus_tag="J1Y83_001431"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA7629590.1"
                     /translation="MMLQLCATVLNVTCVASLKMNEMLINEIDMTGIKKITQTFSLRQ
                     LTFLKGATAKNVRECNLMKNSVAEH"
     gene            27138..28646
                     /gene="putP"
                     /locus_tag="J1Y83_001432"
     CDS             27138..28646
                     /gene="putP"
                     /locus_tag="J1Y83_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/proline symporter PutP"
                     /protein_id="HBA7629591.1"
                     /translation="MAISTPMLVTFCVYIFGMILIGFIAWRSTKNFDDYILGGRSLGP
                     FVTALSAGASDMSGWLLMGLPGAVFLSGISESWIAIGLTLGAWINWKLVAGRLRVHTE
                     YNNNALTLPDYFTGRFEDKSRILRIISALVILLFFTIYCASGIVAGARLFESTFGMSY
                     ETALWAGAAATILYTFIGGFLAVSWTDTVQASLMIFALILTPVIVIISVGGFGDSLEV
                     IKQKSIENVDMLKGLNFVAIISLMGWGLGYFGQPHILARFMAADSHHSIVHARRISMT
                     WMILCLAGAVAVGFFGIAYFNEHPAVAGAVNQNAERVFIELAQILFNPWIAGILLSAI
                     LAAVMSTLSCQLLVCSSAITEDLYKAFLRKQASQKELVWVGRVMVLVVALVAIALAAN
                     PENRVLGLVSYAWAGFGAAFGPVVLFSVMWSRMTRNGALAGMIIGALTVIVWKQFGWL
                     GLYEIIPGFIFGSIGIVVFSLLGKAPSAAMQKRFAEADAHYHSAPPSRLQEG"
     gene            28866..29696
                     /locus_tag="J1Y83_001433"
     CDS             28866..29696
                     /locus_tag="J1Y83_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FTR1 family protein"
                     /protein_id="HBA7629592.1"
                     /translation="MFVPFLIMLREGLEAALIVSLIASYLKRTQRGRWIGVMWIGVLF
                     AAALCLGLGIFINETTGEFPQKEQELFEGIVAVIAVVILTWMVFWMRKVSRNVKVQLE
                     QAVDSALQRGNHHGWALVMMVFFAVAREGLESVFFLLAAFQQDVGIWPPLGAMLGLAT
                     AVVLGFLLYWGGIRLNLGAFFKWTSLFILFVAAGLAAGAIRAFHEAGLWNHFQEIAFD
                     MSAVLSTHSLFGTLMEGIFGYQEAPSVSEVAVWFIYLIPALVAFALPPRAGATASRSA
                     "
     gene            29754..30881
                     /locus_tag="J1Y83_001434"
     CDS             29754..30881
                     /locus_tag="J1Y83_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron uptake system protein EfeO"
                     /protein_id="HBA7629593.1"
                     /translation="MTINFRRNALQLSVAALFSSAFMANAADVPQVKVTVTDKQCEPM
                     TITVNAGKTQFIIQNHSQKALEWEILKGVMVVEERENIAPGFSQKMTANLQPGEYDMT
                     CGLLTNPKGKLIVKGEVTADAAQSDALLSLGGAITAYKAYVMAETTQLVTDTKAFTDA
                     IKAGDIEKAKALYAPTRQHYERIEPIAELFSDLDGSIDAREDDYEQKAADPKFTGFHR
                     LEKALFGDNTTKGMDKYADQLYTDVVDLQKRISELAFPPSKVVGGAAGLIEEVAASKI
                     SGEEDRYSHTDLWDFQANVEGSQKIVDLLRPQLQKVNPELLAKVDANFIKVDTILAKY
                     RTKDGFENYDKLTDADRNALKGPITALAEDLAQLRGVLGLD"
     gene            30887..32158
                     /gene="efeB"
                     /locus_tag="J1Y83_001435"
     CDS             30887..32158
                     /gene="efeB"
                     /locus_tag="J1Y83_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deferrochelatase/peroxidase EfeB"
                     /protein_id="HBA7629594.1"
                     /translation="MQYEDENGVNEPSRRRLLKGIGALALAGSCPVAHAQKTQSAPGT
                     LSPDARNEKQPFYGEHQAGILTPQQAAMMLVAFDVLASDKADLERLFRLLTQRFAFLT
                     QGGAAPETPNPRLPPLDSGILGGYIAPDNLTITLSVGHSLFDERFGLAPQMPKKLQKM
                     TRFPNDSLDAALCHGDVLLQICANTQDTVIHALRDIIKHTPDLLSVRWKREGFISDHA
                     ARSKGKETPINLLGFKDGTANPDSQNAKLMQKVVWVTADQQEPAWTIGGSYQAVRLIQ
                     FRVEFWDRTPLKEQQTIFGRDKQTGAPLGMLHEHDVPDYASDPEGKVIALDSHIRLAN
                     PRTAESESSLMLRRGYSYSLGVTNSGQLDMGLLFVCYQHDLEKGFLTVQKRLNGEALE
                     EYVKPIGGGYFFALPGVKDANDYLGSALLRV"
     gene            32509..33573
                     /gene="phoH"
                     /locus_tag="J1Y83_001436"
     CDS             32509..33573
                     /gene="phoH"
                     /locus_tag="J1Y83_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate starvation-inducible protein PhoH"
                     /protein_id="HBA7629595.1"
                     /translation="MVTSCTGHVLDNQRATTRGDFSSGSHLVTLYFQPHPFFSCVTDA
                     VNGARSRFSAFYPKANYGLQGSQPSDVRAHNRAANGACDEYKQLKVLSMGRQKAVIKA
                     RREAKRVLRRDSRSHKQREEESVTSLVQMSGVEAIGMARDSRDTSPILARNEAQLHYL
                     KAIESKQLIFATGEAGCGKTWISAAKAAEALIHKDVDRIIVTRPVLQADEDLGFLPGD
                     IAEKFAPYFRPVYDVLVRRLGASFMQYCLRPEIGKVEIAPFAYMRGRTFENAVVILDE
                     AQNVTAAQMKMFLTRLGENVTVIVNGDITQCDLPRGVRSGLSDALERFEEDEMVGIVR
                     FGKEDCVRSALCQRTLHAYS"
BASE COUNT         8111 a         8693 c         9074 g         8043 t
ORIGIN      
        1 gcgtttacaa ctattaggta cagagatatt cggttaaatg gattagtttc ttgatgaaaa
       61 agtaacttgc gttataatgt gtggtgttga ctattgatct attccaataa aaccagtcag
      121 ttttaaacaa gcagcgtcat attaagagag ataaacattt gccgctgttg gtccacgcag
      181 gccatttacg cggcaaaatt ccacgcgtaa tcctggtata agcacttctg cgtcgcgggg
      241 agtgaatgcg gaaatatgga cctggacttc tttacgaccg tcggagggga taatgaatcc
      301 tttgccgctt ttgcgatcaa aggttttgac aattcctgtc attttacggg acaaacaaat
      361 tccttactga aaatactgcg ctgcactata cggggttaat aaaataaagc cagcgatatt
      421 taagaccgcc ggacggctaa aataaaattt gcttaatctc aattatcatg cgttaatagc
      481 tacgtcggtt tgaaagacag acagcataca aagtagttta ctaaagcagt tctcattatc
      541 aggcattatc cccttctttt gagtctctct cctgaacact aagtagtttc tgtattaaag
      601 ccctgtttgc cgaaaggccc aaaatgaagg aagtaaaata tgtctaataa aatgactggt
      661 ttagtaaaat ggtttaacgc agataaaggt tttggcttta tcactcctga tgatggcagc
      721 aaagacgttt tcgtccattt cactgcaatc cagagcaatg aattccgcac gctgaacgaa
      781 aatcagaaag ttgaattttc tattgagcag gggcaacgtg gtcccgcggc agcgaacgtt
      841 gttacgctct aaggttgccg ttattactca acatctccat ttccgctgtc catgttgcca
      901 tggttcacag taccgcacat cggcattcga tgtgacggag cgaaatcctt taggagcaaa
      961 gtgcattttt tgtaaatcga cgatgatcac ctttgataac gttgcgctgc aaatacgcac
     1021 tgaccatgcg ccgctggatt tcacaaaata atatcaggct ccctcgtgga gcctttttta
     1081 tatctgcctt atttttcttc aatgctgtac gtatagtaag cgataacctg ttgattattg
     1141 aatctttcgg ggagatggct tataacattt cttacctgac cagggtaccg ggaaccaaca
     1201 ccttactggc gtgttgctgt cttttaagac cagaagaggt taacagtgaa tattgaagag
     1261 ttaaaaaaac aagccgaaac ggaaatcgcc gactttatcg cgcaaaaaat cgccgagctg
     1321 aacaagaata cagggaaaga agtctctgaa attcgcttca ccgcacgaga aaaaatgacc
     1381 gggcttgaaa gttatgatgt caaaatcaaa ataatgtgat tttgtgaaca tcaccccgtg
     1441 cgaggtgatg ttccgcttgt tgctaattta gtgaccaatc attggcgatt gcggaattaa
     1501 gcgttggtac aattcctccg gcaccgggct ttgccatact cccgcataga ttgcgtaacc
     1561 aatcgccgca aacataatcc ccataaccag tagcgtcatt aaccatccag acaacgcaaa
     1621 ggctttttta tttgccgcag gtttttgcag tgaaaaggtc aatgttgagg ctaccggaca
     1681 tgactctacg caagtcatac agccggtaca ttccactgtt cgtacctgaa ttaatttatc
     1741 gaccgggatc cgtgatgggc aactttttgc gcatttgcca caatcgatac aactttcggc
     1801 attgcgacga atcttaaacg gcgataatag cgaaaccacg cccatcagcg cgccatatgg
     1861 gcaaagataa cgacaccagg catggcgaat aaacaggctg gcaatcagca aaacgatcat
     1921 gctgattaat gtcgcggtcc ccatatgacg aaagaaatcg agcattttaa cgtccatcac
     1981 cacgctgtag ggcgacaaca taaaatagtg aatcgcctga gcgggcatca ataacgcgat
     2041 atagagaaaa aaactcaaca gcaaatactt cacgccgcgc agaggaatat ccagccagcg
     2101 gggaaggaca aattgccgac caaacagttt gttaccgaga tcgccgatta attcagaaag
     2161 cgtaccaacc gggcataacc atgaacaaaa ggcctttttg agtaatagac tgatgacgat
     2221 aaaagagacc aataacagca tcgcggcggc gtggacggac ggtaactgac ctgttgcaag
     2281 gctatatttc agattcatca gaccggcaat cggtagccag ccttcgatac ctcccggtct
     2341 ggcgacaaat gtcgtactac ctgccgtttc gtaatagcgc acccaatacc agaacgtgat
     2401 ggcaatataa atattaattg ccaacagtaa taattgcgtc gctttacgcc aggtcgtggc
     2461 attgcgccag tcattccacg gtaatttgcc gcccgtcgtg cctggccgcc gctgccagcg
     2521 ggttcttttt ttctctgcca tgattttgcc agtccgttaa gttgcatacc aaatgccact
     2581 attttagttg ttcttaactg gctgatattg attcaaatcg cgttcaggtc tttcttatta
     2641 accatgcttc cagagcggca acactgcgtg taatttcttc gtgtggaagg ggggcagata
     2701 atggctgctg ctccagttgt gcgcattgct ggctggcgct acgcattccc agactcgagc
     2761 aactgctttt tagctgatgt gcggcacgct ttattttctc gttatcctga ctggcgcggg
     2821 caatgtcgat ttcattgaga agcggcaggg catgttgttt aaataatatc agccattcgt
     2881 ggatcttctc cgcccccatt aaatgagcat cttcattgag ttgcgataca tccagtggtt
     2941 gatcgttatt gacttgcagt tggagatagt gcgccagtaa ttgaccgagc acttcacgcg
     3001 gcaccggttt agggataatc ccgcgaaata gcgaactggt acgctgtcgc agtgtttcgt
     3061 caatgacatg ggcgctaaag ccaatcaaaa ccagtgacgg atattgctgt gccagttgcc
     3121 gggcaagggt aatgccgtcg atatccggca gatcaaaatc caccagtgcg gcagcaaacg
     3181 gttcgctatt ttgcaatgcc tctattgcct gcgcggcatt tcctacggca acgacctgcg
     3241 caccactggt gttcagcatt tcgacagtaa ttcgctgggt gagcgggtta tcttcaatta
     3301 acagcaaacg caaatcgtcc agacgtaccg cctgattgac tgttttgggc acgggtgcag
     3361 tggcaatacg taacggcaag cgtaaacaaa aacagcttcc aacctccggc gtgctggtgg
     3421 cgctcagttc gccgcccatc gcctgggcca gacggctact gatagtcagc cccagcccgg
     3481 ttccgccgcg tttgctgctt acctgaacaa atggctggaa gatttctgcc agtttcgcgg
     3541 gatcaatacc gcaaccgctg tcttccactt cgaccagcca ttgttcgcca tcagtgcgac
     3601 tacgcaggat aatgtgccct tcgtcggtga aacgcagggc gttgctcaac aggttggtga
     3661 tgacctgacg aatacgccgc ggatcgccca ttaacgcagt cggtacatcg tcggcaattt
     3721 ctgttgccag gcgaatcggg cgacctttca cccttccgct cattaattgc agggtacttt
     3781 ccagcagcgg gcgcggttca aagggttcat cgctgaccga aacattcttg ccgcctgctt
     3841 cgatagccga ataatcgaga atatcgttga ggatggtcag caacgattca ccagagtcgg
     3901 taatcgcccg caaatcatcg cgctgggcgt taagtgcggg gttatctgcc agcagttgcg
     3961 cggtgccgag aataccgtac agcggtgtgc ggatctcatg gctcatcgcc gccagaaacg
     4021 ccgattttgc ctggctggct ttttctgctt ccgcccgtgc ctgtcggtgt tctatcacca
     4081 gttcctgcaa ttcagctgta cgcgctttga cctgcgccgc cagctgttcg cggtggcgat
     4141 tcagtgcatg aacactgctg cgaaacgcat ccatcagccg cccgatggtg tccagttccc
     4201 gcacgccagc ggtttccggg aaaggggagt cgatatcacc atccagcaag cgttgcagcg
     4261 cctgcgtttg ttctgcaaga gggcgcgtga ctgagcgata aaccacgcgc cagaggatca
     4321 gaatcagtgc gcaaagagaa actattccca gcaataacag gctgtattgc ccgcgtgcac
     4381 tggctttttc cagatgcgcc agcccgtgct gattacgcag ctcaatggtg tcgaccagct
     4441 gactgacttc gctactaaac tgcgcgaatt gggcgatgtt attttgggcg agagtttgca
     4501 ggtgattgct gatttcactg tcctgctgat acagcgccag caaatcgcta tattggctaa
     4561 cggtagttaa cgttgttgct acctgagtac gaacacccgg atcttcaatg cgtatttgcc
     4621 gacgttgcag aattttcacc gcattattga gctgcttttc cagcgttggc gcatttttct
     4681 ggatctgttc cagccccaga ttcatcacca tttgctgcac ccgcagggcg ctaaggcgca
     4741 gttcattcat ctggttaaca tactcaagat cgatatcaat cagccgatcg agtgcacttt
     4801 ctgcggcctg acgctgatgt tgttcgatca aatcgtaaat cccggcctgg gtcgctccgg
     4861 cggaggttgc cgcattattc gcctgacctt gcgccaggcg tgcgatctca tcggcggcgg
     4921 cgactatctg ctgactgagt tgctgttgtt gctggcgcag ttgcaaacgc tgtcccacca
     4981 gttccccttg ctgacgtaac gaacgggaga tctcctgctc ctgttgttca atagcggtgg
     5041 tgtcaaaacc ttgttcccgt aacgcttgca gcaacgcatt aatcttcagg ctttgtgcgg
     5101 tgagcattcg cccctgcgcc tgccacatct tttcgttatc ggcactggtc aggttctgcg
     5161 cggcgaaaag ttcccaggcg ctggcttcgc tcaactggcg cgccatattc atggtaggaa
     5221 tcaatgcctg agtgttgtct ttttccacct ggctgataaa gcgcaggttg taccatccca
     5281 ccagggtact ggtcagggtt aacagcgcca tcagggcaaa gcccatccag agtcttcggg
     5341 tcagggttaa attcacggtc ggtgcacttt gggtgaaaaa ggttgagtcg caaagcggaa
     5401 tgcatctagc ataaagcctt attattgatg aggctatcat gcgcgtactg ctattgttac
     5461 tactttccct tttcatgtta tcggcatttt cggctgataa cctgttgcgc tggcatgatg
     5521 cacagcatta cacggtgcaa gcctctacgc cgctaaaagc caaacgcgca tggaaactgt
     5581 gcgcgcttta tcccagtttg aaagattcat actggttatc gttgaactat ggtatgcagg
     5641 aggctgctcg ccgctacggt gtggatttaa aagtgctgga ggcaggcggc tacagccagt
     5701 tggctacgca gcaagcacaa atcgaccagt gtaaacagtg gggcgcagag gccattttgc
     5761 tcggtagtag cacgacgtca tttcccgatc tgcaaaagca ggtagcaaat ctgccggtga
     5821 tcgaactggt gaatgctatt gatgctcccc aggtgaaaag ccgcgttggt gtgccctggt
     5881 ttcagatggg ctatcaacca gggcgatatc tggtgcaatg ggcgcacggt aaaccactga
     5941 acgtgctgtt gatgcccggc cccgataacg ccgggggcag taaggagatg gtagagggtt
     6001 ttcgcgcagc cattgccgga agcccggtac gtattgttga tattgcgctc ggtgataacg
     6061 atattgaaat ccagcgtaac ctgttgcagg agatgctgga acgccatcca gaaatcgacg
     6121 tcgttgccgg aacggccatt gcggcagagg cggcaatggg ggaagggcgc aacctgaaaa
     6181 cgccgcttac cgtggtgtcg ttttatcttt cacatcaggt gtatcgcggg ttgaagcggg
     6241 gaagagtgat tatggcagcc agcgatcaaa tggtctggca gggggaactg gcggttgagc
     6301 aggctatcag gcaattacag gggcaatcgg tgtctgataa tgtcagccca ccgattttag
     6361 tcatgacgcc gaaaaatgcc gaccgcgaac atatccgccg ctcgctgtca ccagggggat
     6421 ttcgtccggt ctatttttat cagcacacat cagcggctaa gaaataacct tcgccatgtt
     6481 gtgtcaccag taaatctgca ctgagtttat ggcgtaaacg acgaattaac acatcgacgg
     6541 tgcgcaggtc agggttttcc acccgacgcg cagagagcat acgcagcaga cgttcacggc
     6601 tgagaatttc gccgggattg gtcacaaacg ccaccagcat ttcatactct gcgcgggtca
     6661 gtttaatcgg ctctccatcc cgctccagcg tatggcgcga cacattcagg caataaccgg
     6721 caaagcgata gcagttgtcc tgagtgtgcg gttgcgcctg tcgcgcgagg tcgattcgcc
     6781 agagcaaatt tttcactcgt accaccagtt cgcgcagctc cagcggtttg gtgacgtaat
     6841 cgtctgcgcc catttccagc ccaacaatac ggtcaatccg atcgctgcgt ccggtaacca
     6901 gaataatccc caccgttgag cgttctcgca gggcgcgggt taacatcagg ccattttcat
     6961 cgggcaaatt gatatccagc agaatcaaat ccaccggctg attctgcata atttcccgca
     7021 gcccggcacc gctcgccgta acggaaacgg tatacccctc ctgagtgaag taagactgta
     7081 atcgcgcctg ggtaaccggc tcatcttcaa caataacaat gtgatgtggc atcagagggt
     7141 tttactcatt ctgttcatat ctgttcatat tctgccgtaa gctattcatc ctgaccagtg
     7201 ccgctgttca tatttgctca ttaagatcgc ttcactaaac cataatttta caggggctat
     7261 tatgcggaaa ctctggaacg cgctacgccg acccagtgct cgttggtcgg tactggcgct
     7321 ggtcgctact gggattgtga ttggcattgc gctgattgta ttgccacacg ttgggatcaa
     7381 agtcaccagc acaaccgaat tttgtgtcag ttgccacagt atgcaaccgg tgtatgaaga
     7441 atataaacag tcggtgcatt tccagaacgc ctccggcgtg cgagctgaat gtcatgactg
     7501 tcatatcccg ccggatattc caggcatggt gaagcgcaaa ctggaagcga gcaacgacat
     7561 ttaccagacc tttattgccc actccattga tacacctgag aaatttgaag ccaaacgcgc
     7621 ggaacttgcc gagcgtgaat gggcgcgaat gaaagaaaac aactcggcaa cctgccgctc
     7681 ctgccataac tacgatgcga tggatcatgc gaagcagcat cctgaagcgg cgcgtcagat
     7741 gaaggtggca gcgaaagata atcaatcctg catcgactgt cataaaggta ttgcccacca
     7801 gttaccggat atgagtagtg gcttccgtaa gcagttcgat gagctgcgcg ccagtgctaa
     7861 tgatagtggt gacacgctgt actccattga tattaagccg atttatgcgg cgaaaggcga
     7921 taaagaagcg tctggttctc tgctgcctgc ttcggaagtg aaagtcctta aacgtgacgg
     7981 cgactggctg caaattgaaa tcactggctg gacggaaagt gcaggacgtc agcgtgtact
     8041 cacccaattc ccaggtaaac gcatctttgt tgcctcgatt cgtggtgatg tgcagcagca
     8101 ggtgaaaaca ctggagaaaa ccaccgttgc cgacaccaat accgagtgga gcaagttgca
     8161 ggccactgcg tggatgaaga aaggcgacat ggtgaacgat atcaaaccga tctgggctta
     8221 tgcggattcg ctgtacaacg gcacctgtaa ccagtgccac ggcgcaccgg aaatatccca
     8281 ctttgacgct aacggttgga tcggcacgct caacggcatg attggcttta ccagcctcga
     8341 taaacgtgaa gaacgcacct tgttgaaata tctgcaaatg aatgcgtctg acaccgcagg
     8401 taaagctcac ggcgataaga aggaagaaaa ataatgaaca ataacgatct ctttcaggca
     8461 tcacgtcggc gttttctggc acaactcggc ggcttaaccg tcgccgggat gctggggccg
     8521 tcattgttaa cgccgcgccg tgcgactgcg gcgcaagcgg cgactgaggc tgtcatctcg
     8581 aaagagggca ttcttaccgg gtcgcactgg ggggctatcc gcgcgacggt gaaggatggt
     8641 cgctttgtgg cagcaaaacc gttcgaactg gataaatatc cgtcgaaaat gatcgccgga
     8701 ttgccggatc atgtacacaa cgcggcgcgt attcgttacc cgatggtacg cgtggactgg
     8761 ctgcgtaagc gccatctgag cgacacctcc cagcgcggtg ataaccgttt tgtgcgcgtg
     8821 agctgggatg aagccctcga catgttctat gaagaactgg aacgcgtaca gaaaactcac
     8881 gggccgagtg ccttgctgac cgccagtggt tggcaatcga cggggatgtt ccataacgct
     8941 tcggggatgc tggcgaaagc tattgccttg catggtaata gcgttggtac gggcggagat
     9001 tactctaccg gtgctgcgca ggtgatcttg ccgcgcgtgg ttggctcaat ggaagtgtat
     9061 gaacagcaaa cctcctggcc gctggtattg cagaacagca aaaccattgt gctgtggggc
     9121 tccgatttgc tgaaaaacca gcaagcgaac tggtggtgcc cggatcacga tgtttatgaa
     9181 tattacgagc agttgaaagc gaaagtcgcc gccggtgaaa ttgaggtcat cagcatcgat
     9241 ccggttgtta catccaccca tgagtatctg gggcgcgagc atgtgaagca cattgcggtt
     9301 aacccgcaaa ctgacgtgcc gctgcaactg gcgctggcgc atacgctgta cagtgaaaac
     9361 ctgtacgaca aaaacttcct cgctaactac tgtgtaggtt ttgagcagtt cctgccgtat
     9421 ctgctgggtg agaaagacgg tcagccgaaa gatgccgcat gggcagaaaa actgaccggc
     9481 attgatgccg aaaccattcg tgggctggca cggcagatgg cggcgaacag aacgcagatt
     9541 attgctggct ggtgcgtaca gcgtatgcag cacggtgaac agtgggcgtg gatgattgtc
     9601 gttctggcgg cgatgctggg gcaaattggc ctgccaggtg gtggttttgg ttttggctgg
     9661 cactataacg gcgcaggcac gccggggcgt aaaggcgtta ttttgagtgg tttctccggc
     9721 tctacgtcga ttccgcctgt tcacgacaac agtgactaca aaggctacag cagcaccatt
     9781 ccgattgccc gttttatcga tgcgatcctc gaaccgggga aagtgatcaa ctggaacggt
     9841 aaatcggtaa aactgccgcc gctgaaaatg tgtatttttg ccggaactaa cccgttccat
     9901 cgccatcagc agatcaaccg cattattgaa ggctggcgca agctggaaac ggttatcgcc
     9961 atagataacc agtggacctc aacctgccgc tttgccgata tcgtgctgcc tgcgaccacg
    10021 cagtttgagc gtaacgatct cgaccagtac ggcaaccact ccaatcgtgg catcatcgcc
    10081 atgaaacagg tcgtgccacc gcagttcgag gcgcgcaacg actttgatat tttccgcgag
    10141 ctgtgccgcc gctttaatcg cgaagaagcc tttaccgaag ggctggacga gatgggctgg
    10201 ctgaaacgca tctggcagga aggtgtacaa caaggcaaag gacgcggcgt tcatctgcca
    10261 gcgtttgatg acttctggaa taacaaagag tacgtcgagt ttgaccatcc gcagatgttt
    10321 gttcgccacc aggcattccg cgaagatccg gatctcgaac cgctgggcac gccgagtggc
    10381 ctgattgaga tctactcgaa aaccatcgcc gacatgaact acgacgattg tcaggggcat
    10441 ccgatgtggt ttgagaaaat cgaacgctcc cacggtgggc ctggctcgca gaagtatccg
    10501 ttgcatctgc aatctgtgca tccggatttc cgacttcact cgcagttatg tgagtcggaa
    10561 actctgcgtc agcaatatac cgtagcgggt aaagagccag tatttattag cccgcaggat
    10621 gccagcgcgc gcggtattcg tcacggtgat gtggtacgcg tctttaacgt tcgtggtcag
    10681 gtgttggctg gagcagtggt ttctgaccgt tatgcacccg gcgtggcgcg aattcacgaa
    10741 ggggcatggt acgatccgga caaaggcggc gagccagggg cgctgtgcaa atacggtaac
    10801 cccaacgtgt tgaccatcga catcggtact tcgcagctgg cgcaggcgac cagtgcgcac
    10861 actacgctgg tggaaattga gaagtacaac ggaacagtgg agcaggtgac ggcgtttaac
    10921 ggccccgtgg agatggtggc gcagtgcgaa tatgttcccg cgtcgcaggt gaaatcatga
    10981 ccacgctgac agcacaacag attgcctgtg tttacgcctg gctggcgcag ttgttctccc
    11041 gtgaactgga cgatgaacaa ctgacgcaaa tcgccagtgc gcagatggct gaatggtttt
    11101 cgttgctgaa aagcgaacct ccgctcactg cggcggtgaa tgggctggaa aacagtattg
    11161 ccactctgac agtacgtgac gatgcccgtc tggaactggc cgctgacttt tgcggcctgt
    11221 ttctgatgac cgacaaacaa gcggcgctgc cgtatgcatc ggcctacaaa caggacgagc
    11281 aagagattaa acgcttgtta gttgaggcgg ggatggaaac cagcggcaat ttcaacgaac
    11341 cggcagatca tctggcgatc tatctcgagt tgttgagtca tctgcatttt tcgctgggag
    11401 aggggaccgt tcctgcgcga agaatcgacg gtttgcggca aaaaacactg acggcgctgc
    11461 ggcaatggtt accggaattt gttgcgcgtt gccatcagta tgaccgcttt ggtttttacg
    11521 cagcactaag ccagttgttg caggtgttgg tggagtgcga caaccaaaag ggataatgcc
    11581 gatttgtagg cctgataaga cgcgttaagc gtcgcatcag gcattatggc gcagttgccg
    11641 gatgcggcga gaacgcctta tccggcctac gggaacagtg ctttttgtgg gcctgataag
    11701 atgcgttaag cgtcgcatca ggcattatgg cgcagttgcc ggatgcggca tgaacgcctt
    11761 atccggccta cgggaacagt gctctttgta ggcctgataa gacgcgttaa gcgtcgcatc
    11821 aggcaacaaa ctcacgggtg agccacaaac cgggaaagcc gctggcgcag caggcggttt
    11881 tcctgcttca ggtgcgcaat atcatccatt aacgtcagcg ccaccgcgat ccccggccag
    11941 tccagagcca gttcatgacg caggcgtacc gcgcgttgca ccacaatggc ggcatggtcg
    12001 tcaaataccc aggttgtctc ctggatctca ctcggttcaa ccacccccaa accgacaatt
    12061 tcattcaact cctcttcaga gatgccggta tgcaggcaaa attcggtaat agtaaaagtc
    12121 accgtaacat tagccattat gctttccccc aatctttacg tggatcaaaa gacgactggg
    12181 cgtctgccag ttgctgccac agcgcggcag tgttttcatc cggtttcggc ggcatcacga
    12241 ttttcagtac cgcatacaga tcgccggtct gttttttgct caccagacct ttgcctttaa
    12301 cgcgcaatcg ttgcccggcc tggctgccag gcgggatagt cagcaaaatg ctttctttca
    12361 gtgttggaac ggtgacttta gtacccagcg ccgcttccca cgggctaacc ggcaccacta
    12421 tttccagatc ctggccgaca atatcaaaca gcggatgtgg cgcaatgtga atcaccagcc
    12481 acaggtcgcc atttggaccg ccgttttcgc ccggcgtccc ctggcctttc aggcggatgc
    12541 gttgaccatt gccgacgccc gccgggatct tcacattcag cgttttcggg atttcctgtt
    12601 cgatcatgcc aaaggcgtta taaaccggca ggttatagct gatggtacgc ttatgctcag
    12661 taagcgtttc ttcgaggaat accgccactt cgatttcaat atcgtggccg cgtgtggcgg
    12721 ggcgttgacg gctctggcgg gcatgctgac cgaaaattga cgagaagata tcgtcaaaat
    12781 cttcggcgtt aaaactctga ccgtcgccat gatggaactg acggttaaat tgcggatcgt
    12841 tgcgatgttg ccacatctga tcatactcag cgcgacgttg ttcatcactt aacacttccc
    12901 aggcttcagc gacctctttg aagcgggctt cggcatcagg ttctttgctg acatcaggat
    12961 ggtatttgcg ggccagtcga cgataggcgg tcttgattgt cttgagatcg tccgtcggtt
    13021 tcacgcccat gatggcgtaa taatccttta attccatagc gttatctcgc gtaaatcaac
    13081 acaaattgaa ggaacccctg taaggtaact cctataagtg tagggtaatc ctcaaaattt
    13141 catatgccaa cacagaatat gttattgaaa tcatcgcgga ggggaggtcg cctcaaggtg
    13201 ggaggaacat attttattta cctcactcct gaacttttgg ctcatgctta caagttaaac
    13261 aataaggaaa ggaatgcaca actcgactat ttaaatcgtt atgcaattta atgataaaga
    13321 catgtgctaa aaaccatttc tattagtctc acattctttt tttattggta atggtaacgc
    13381 cgctgatttt ctttatagct actgatgtaa tcattagcgg cgtaaacgag gccggggcta
    13441 atcaggcata gtttgcgtca aaacttgcct gtttttaaag gggaatatag aaaaacaggc
    13501 gttcaacgag ccattttgcg aacctgttcc cggaaaaatg acatatttct gtcacactct
    13561 ttaattattg ataacaaaag aggtgtgagg aatgaacaaa acgctaatcg ccgcaactgt
    13621 ggcagggata gttttactcg cttcaaatgc tcaggcacaa accgtaccgg aaggctatca
    13681 gctacagcaa gtgctcatga tgagccgcca taacttacgt gcgccgctgg cgaacaatgg
    13741 cagtgtgctg gagcagtcga cgccgaataa atggcctgaa tgggacgtcc ccggtgggca
    13801 gctcaccact aaagggggcg tgctcgaagt gtatatgggc cattatatgc gtgaatggct
    13861 ggcagaacag gggatggtga aatcggggga atgcccgcca ccggacaccg tttatgccta
    13921 tgccaatagt ctgcaacgca ccgtcgcgac cgcgcagttc tttattactg gcgcattccc
    13981 tgggtgtgat attcctgttc atcaccagga aaaaatgggc actatggacc ccacctttaa
    14041 cccagtgatc accgatgatt ccgccgcatt cagtgaacag gcggtggcgg caatggagaa
    14101 agagctcagc aaactccagc ttaccgacag ctacaagcta ctggaacaaa tcgttaacta
    14161 taaagattct cctgcgtgta aagagaagca acaatgttcg cttgtagatg gcaaaaatac
    14221 ctttagcgcg aagtatcaac aagaaccggg cgtttccggg ccgctgaaag tcggcaactc
    14281 gctggtagat gcgtttactt tgcaatacta cgaaggtttt cctatggatc aggtggcctg
    14341 gggagaaatc aaatctgacc agcagtggaa ggtgttgtcg aagctgaaaa acggttacca
    14401 ggacagcctg tttacctcac cggaagtggc gcgcaatgtt gcgaaaccgc tggtcagtta
    14461 tatcgacaaa gccctggtca ccgatcgcac cagtgctccg aaaatcacgg tattagttgg
    14521 gcacgactcc aacattgcct ctctgttaac ggcgctggat ttcaaaccgt atcagttgca
    14581 tgaccagaac gaacgcacgc cgattggcgg caaaatcgtt ttccagcgtt ggcatgacag
    14641 caaagccaat cgcgacctga tgaaaattga atatgtgtac cagagtgcgg aacagttacg
    14701 taatgccgat gcgttaaccc tgcaggcacc tgcgcagcgc gtgacgctgg aattaagtgg
    14761 ttgcccgata gatgccaacg gtttttgccc aatggataag tttgatcgcg tattgaatga
    14821 agcggtgaaa taacagaaaa ctcccccgcg tgaagcgggg gagtcgctgg ttaaacgttt
    14881 ttacgttcga tggtctgttc gccccaaaaa agcgaatctt tatcggtctt agcaaaggct
    14941 ttgactaaca cttcatcgct accttcttcc caaatctttt ccgccatttt ttcgtcgtac
    15001 ccggcgactt cgaaaatggc ctcggctatt tccggcgacg tattgcgcag agatgcccat
    15061 tcaccgacgt gatgagcttt cgcttcttga gttggcatgc gtatcctcct gttgaagatt
    15121 agccgttaag tttaactgcc agacctgcga catattcccc ttgataacga gcaatagaca
    15181 gttcttcctg gctgggctgg cgtgaaccgt caccgcctgc gatggtggtt gcgccgtacg
    15241 gcgtaccgcc gcgaacctgt gaaacgtcaa ataattcctg cgctgcgtag ccaatgggaa
    15301 caatcaccat gccgtgatgc gcaagggtcg tccaggtgga tgtgatggtt tgttcctgac
    15361 cgccgccagt accggtggaa ctaaagacgc tcgccagttt tccgtatagt gcgccggaag
    15421 cccacaggcc gcccgtctgg tcgaggaagg tacgcatttg accggacatg ttaccaaagc
    15481 gggtaggtgt accaaaaata atggcgtcgt aatcggccag ttcttgcggg gttgcaaccg
    15541 gtgcagtttg cgttttaccg cctgcttttt caaataattg cggcggcatg gtttccggta
    15601 cacgcttaac gacaacttct gcgccatcca ctttgcttgc accctcagca actgcgcgtg
    15661 ccatcgtttc aatatgtccg tacatggaat aataaagcac cagaacttta gccatttcta
    15721 accactcctc gtgttatctc tattccgtag cgattcgcta ccacttattt aaagataaga
    15781 cgtccttttc agagtgcaaa tttcacaacc acttatttga tttataacaa ctttcacaag
    15841 cacgtaattt tgtcgcaaaa tgacacattt ttatctcatc gcgttttttt aatcataaga
    15901 gcagcttatg gataattatt ggagatgata tctattctcg ctaagaagct gttgcaggat
    15961 attaccaaac gcgggtctgc ccgcgtcagt tcactaagct tagtcccacg tagcgaaaat
    16021 atggcagccg ccatacgccg cgttaattct atgcaatatg atgtctatac ccagacggag
    16081 gtcagtaatg gcaaaccatc gaggcggttc cggcaatttt gcagaagacc gcgaaagagc
    16141 atcagaagca ggtaaaaaag gtggacagca cagcgggggt aatttcaaaa atgacccgca
    16201 gcgcgcatct gaagcaggta aaaaaggtgg caagagcagt cacggtaaaa gtgacaacta
    16261 gccgggctaa tcaatgacgc atgcattttt gtctgtagct cgtcaaaaag ccatcaccgc
    16321 cggttacccc ggcggttttc tgtttatggc ttctgatgaa caacttctgg cggtggaacg
    16381 tcaaccaacc ttctgcttaa caacgcattg agtaaaatcg cgccaaaggt ggctgtaccg
    16441 atccctccca acgtaaaacc gcccagcgtg agagcaaaat cacccgcacc cagcactaag
    16501 gttactgcga ccataatcaa attaccgttc tggcttaaat ccacacggtt ttgcacccag
    16561 atccgtgcgc ctgcgacggc aatcagcccg aacacaacaa ttgatgcacc accaatcacc
    16621 gcagccggaa tagtatggat cagcgcgcca aatttcggcg aaaagcccaa cagcatggcg
    16681 atgacggcag cggcaacaaa caccagcgtc gagtagactt ttgtcacggc catcacacca
    16741 atattctcgg cgtaggtggt tacgccgcta ccgccgacag agccagaaag catcgtcgcc
    16801 agcccatcgc ctacgaatgc ccgccccata tacgggtcca tattgcgtcc ggtcatcccg
    16861 gcgaccgcct tgagatgacc taagttttcc gccaccagaa tcaccgccac gggcgcaatc
    16921 agcatcattg cctgaccatt aaaagcagga gtggaaaaat gtggcagacc gaaccaggca
    16981 gcatggctga cgagagtaaa atcgacggct tttcccagcc ctaaaacgtt ggtcatcacg
    17041 ccatacagca gacaggcaac aattaatcct acgagaatca ataatcgctg gatcatgcca
    17101 cgggtaaata cggccaccag cccaatacac agcaccgtca ttaccgccat ccagctatca
    17161 aaggccgaag ccgatacatt tttcactgcg ataggcgcta agttcaggcc aatcgccatc
    17221 actaccgcac ccgtcacaac cggcggcatt agtcgttcaa tccagcgcgt accgattttc
    17281 atcaccacca ggccaatgac ggtataaacc agcccacagg cgataatccc gcccagcgcg
    17341 atgccgatat tcggattaat tccctgacca ttaaagcccg tcgcggcaat caccacgccg
    17401 acaaaagccg cgctggaacc gagataactg ggcacgcgcc cgccggtaat aaagaaaaac
    17461 agtaacgtgc cgatccccga cattaaaatg gaaagattgg gatccagccc catcagaatc
    17521 ggcattaaca ccgtcgcgcc aaacatcgcc accgcgtgtt gaacgcccat tactgccgtt
    17581 tgagcaaacg gcaatcgttc atccggcgcg accacgccgc tctctgtaga ggtcgatttt
    17641 aactgccagt gaggaaaacc gaacattgcc atcagctgtc tccttaagga ggttaacaag
    17701 cagggcgcat cagcgcgtga taactgcgat cgaaccacac cagcccgtag ggtgtggcgt
    17761 gacgatgaat cgcttcgatg gcgcaaaaca aaatgtcgtg ggtgccgacg ctcaccacct
    17821 ggctgatacg gcagtcaaac gaaaccagtg cctcttccag ttgcgggcat ccggtcacac
    17881 cagtctgcca gcgggcggcg gcaaagcggt gttccatggg cgttttgccg ccaaaaaggt
    17941 ttgaaagcga ctcctgcccg gcgctaagtg tatttacaca cagcgttcga ttttcattga
    18001 ataccggcca gacggacgcc ccacgattca ggcacaccag taatgtgggc ggcgtgtcgg
    18061 tcacgctgca gacggcgctg gcggtgaacc cggcgcgccc ggctggaccg tccgtagtga
    18121 tgatattgac cgccgcgccc atacaggaca tcgcatcgcg aaaggtttgt tgatcgacaa
    18181 tgttcattgt ttgctcctta caacagcccg caggcttctt caaaggacag acgtggcagg
    18241 cgcgcaaaaa gtttgctgga atcgccatag ccgatattaa tcagcagatt gctcttcagc
    18301 gtgctgcccg caaaaaaggc gtcgtcaacg tattgacggt caaagcccga catcgggccg
    18361 gtatccagcc ccagcgcacg gcaggcgacg atcagatagg ccgcctgcat ggaactgttg
    18421 cgaaacgctg tttcttcggc aagttgtggg ctggaggtaa accaactgcg ggcatcgccg
    18481 tgaggaaaca gctgtggtaa ccgttcataa aattcactgt cccaggcgac gatagcggtg
    18541 acgggcgcgg tcagggtttt ttgcagattg ccgctggaaa gcgccgggcg cagacgttct
    18601 tttccttctg cagtgcggat aaacacgatc cgtgccggag aacagttagc tgatgtcggc
    18661 ccccatttca tctgggcata aatctcccgt aacgtctcat cgctgacggg gggctcccgc
    18721 cagccgttgt gagtgcgggc atcggtgaac agggtgctaa gcgcacctgg gctaacggct
    18781 tcgttcatag caattcctta cagagcggct tcacggtgat gtaacaggct ggcaagcccg
    18841 ttgagtaaca gagcattaaa cgtttcggga tcggtcacgt tgcaggcgtg tccgccatag
    18901 cgcatcacca ttttctggct atcgggcaga gcggcatgaa gttcactgga acatgctgat
    18961 ggcaccagca gatcatcact ggcgcagatg atttgcaccg ggcagcggat acgatccgca
    19021 tggcgactaa agtcagcgcg tttgagggcg ttaagtcgac gcagtaaatt atttttgccc
    19081 tgaaaatgcg ccagtgccag cgcgtcttct gcctcaaggc gaggtgcgcg ggccgccatc
    19141 cagtcggcgg gatagaggaa caacggctgc gcttccaccc atgcctgcgc gccgccgcta
    19201 tgtagtaact gttcgcgaac ctgaaaacag cggcgcgtat gggcgtttat tcgtagccag
    19261 ccgttaacgc tgaccagcac agttaccgac gcgggataat ccagcgccag ctgcattccc
    19321 accagcgcac cgagcgcatg gccgatcacc gcgtaacgct caatccctgc ggctaccagc
    19381 gcctgatgca gttccgctgc catctgggcg atactgtaat cttctgccag cgtgtcggga
    19441 ttattgccgg tgccgcgctg gtcgtaacag actacctgat actcctgctc cagcaccgcc
    19501 agttgcggta accagtaact gccgctaccg ccaagacccg aaatcaacac cactacgggc
    19561 gcatcagcat aagggggagg tgagagtgaa agtttcatcg cggcctcact tggcgatatg
    19621 cgcaattgtg gcgatttcca ccagcgcgtc aggttttacc agtccgcact gaatgcagaa
    19681 tcgcgccggt ttgtcacccg ggaaaaactc ggcgtagatt tcgttaatcg cggcgtaatt
    19741 tttccagtcg gtaataaaga tgctgttgaa ggtcacatcc gccatcgtgc cacccgccgt
    19801 ctcgatgacc ttgcgaatgg tttccagaac gtggcgggtt tgcgcctttg ggtcatcggc
    19861 aaacagcacg ttgttatgtt gatcaaaagc cagcgtaccg gagacataca ccacgccatc
    19921 agccagcgtg ccgggaacga agggggccag cggtgcgctg ctgccagcag gaataattac
    19981 ggattttggc atcgttaaac tccttaagcg atacgagcaa aggacgtgga agaaagcgcg
    20041 tcgcagaacg tttcgacgtc gctgacccag ccaaaaaagg tttcgatatt gaacaacgcg
    20101 gctttctgtg caaattccgg ccccgcctgg tgtgttgcgt cttccagcac cacgccgaaa
    20161 tactccagaa aaaagccgtc gcgtagcgtc gattcgacgc agacgttggt agcgataccg
    20221 gtgaaaacca gatggcgtat tccacggctg cgcaaaatgc tgtccagcgg cgtattgaag
    20281 aaaccgctgt agcgcggctt cggcagcaca atatcgccag gctgcggcac cagttcatcc
    20341 accagttgat aatcccagga gcctttcgcc agcaatttcc cctgcagctg cggctgatta
    20401 cgcatggttt tcagggcgtt cgatttatga aaattcggtg agccggggcc gccagcctcg
    20461 acatactgtt catcccagcc attttgaaac cagatgatca gcatccctgc cgttcgcgct
    20521 gcggtcacgg cggtttgaat gttggcaatg accgggcgag tggttgagac atcaaacccg
    20581 gcgagatcta agtagccgcc tggcgtggca taggcatttt gcatatccac cacgatcagc
    20641 gcagtttgct gcggatcgaa ggtaatggct tccggtcgtg cggttaaggt cgtcatcatg
    20701 ccacctcctg agtcagcgca gggagatggg cgcggcactg catcagtggt tgaatgcgct
    20761 cgccgaagtt ttcgattccc gacagaaaat catcgaaggt caacagcacg ccttcggcac
    20821 caggcacgct tgcgacttca tctaacatgc gcgcgacact ggcgtaagaa ccgactaacg
    20881 tccccatatt gatgtttacc gccgaagtgg gatcggccat ctgacggacg ttggtgtctg
    20941 taccggagcg ggtatctttc tgactttgtt cggttagcca gcttaacgcc tcttcatccg
    21001 cgcccgcttt gtagtgttcc catttggcgc gagcggcatc gtcggtttcg tcggcaatca
    21061 ccataaacaa cacataagag ccaacgtcgc gtccggtttg ctctgcggcc tgtttcatcc
    21121 gcgcagcggt cggggcgaaa gccgtgggtg tatttacgcc tttgccgaaa cagaagttaa
    21181 aatcggcata ccgggcggag aacgccatgc cagcgtcgct ttgcccggcg cagatcactt
    21241 tcatgggtac actcggttgc ggactgacgc gacaatcatt catggtgaaa aaatcgcctt
    21301 taaaatcgct tttgcccgtg ccccacaggt cgcgcagcac ctgaacatat tcggtgagat
    21361 agtcgtaacg acgggagaaa tagtcatcgc caggccagat acccatctgc tcatactcgg
    21421 gcttttgcca gccagtcacg aggttgacgc caaaacgccc gccagagatg gagtcgatgg
    21481 ttgcggccat acgggcgacg attgccggag gtaacgttaa ggtggcagct gtcgcgtata
    21541 tctgaatgcg tgaggtgacg gctgccagcc ccgccatcaa ggtgaacgac tcaaggttgt
    21601 gatcccagaa ctcagttttg ccgccaaagc cacgcagttt gatcatcgac agggcgaaat
    21661 cgaaatggta gtgctccgct ttttgcacaa tggctttatt cagttcaaat gtcggcatgt
    21721 actgcggcgc gtgggtcgaa atgagccagc cgttgttgcc aataggtacg aatacgccaa
    21781 ttttcatcat caacctctct tcgtctcgta aagtgaaagt cagacggggc gctgcatcct
    21841 gcatatcctt ttcagccgcg taatggcttg tttgcaaagc ggatgccagt ttttaaaaag
    21901 tgaatgttat taatgtgtta atgttaagtt gtctaaaatg cctgaaaaaa agtggactaa
    21961 acggtcaaaa cagttgcaca taaaacatgc attcgtgcgc gatgagagtg cagaaggtcg
    22021 aggccgggcg ggggttttgc tatcctgttg ccaatcaaca agaggggaga gcgcatgacg
    22081 caaggcgcag tgaaaacaac gggtaagcgt tcgcgcgcag taagcgcgaa gaaaaaagcg
    22141 attcttagcg cagcactgga cactttttca caattcggtt ttcacggcac aaggctggag
    22201 cagatcgcgg agttggcggg tgtttcaaaa accaatctgc tgtattactt tccgtcaaaa
    22261 gaggcgctgt atatagccgt actgcggcag attctcgata tctggctggc gccgttaaaa
    22321 gcgtttcgag aagatttcgc cccgctggcg gcgatcaaag aatacatccg tctgaagctg
    22381 gaagtttccc gcgattatcc gcaggcttcg cgcctgttct gtatggagat gctggcaggt
    22441 gcaccgctgt taatggatga actgacgggc gatttgaagg cattaattga tgagaaatcg
    22501 gcgctcattg ccggttgggt taaaagcggc aaactcgcgc cgattgaccc gcagcatttg
    22561 atttttatga tttgggcttc cactcaacat tacgccgatt ttgcccctca ggtggaggcg
    22621 gtgacaggcg cgacgttgcg cgatgaggtg tttttcaatc aaacggttga aaacgtgcag
    22681 cggattatta ttgaggggat tcgaccacgt taaagatgcc ggaggaggtt gtaacatcct
    22741 ccggctacct gtttaaccga tcgtcattaa actggcgtta ccgccagcag cagcggtatt
    22801 cacactcagc gaacgctcga tatacagccg ttccagaagg atattgcttt cgccacgggc
    22861 aaaaccctgc accgaaacaa ttgcaccatc ccgcgcggca acggcttcac acaatgcgcg
    22921 aagctgatcc gaatcaccgt ggaagatcac cgcatcaaac ggttgagcgg ttatattttc
    22981 cgctttcgcc agttgaatac gttcgctgac tgccgatggc aatgccttca ctaactgacg
    23041 atgcagcgca tcatccggcc acagcacctg gctacccacc gccagcacgg cggcgagctg
    23101 agtcagcgca tcctgttcat catccgcaat acacaacacg cgctcacgcg gcagcagcgt
    23161 ccaggtgttg cgttcacccg tcggccctgg caacaatcgt tgtgttcctg cctgcgccaa
    23221 ctcgccatat tgcgtacata acgcctgcaa ttctggacgg tttgccgccc attcccgcag
    23281 tgcatttagc ggctgagtca atgcggcttt caactgcgca tcgaccggat actccgcatc
    23341 ctgacgcgcg agcgtcaccg ccagcgcact ttccgggcga ttcgccagca gacggtagag
    23401 atagagcgga ccgcctgctt tcggcccggt accggacaac ccttcgccgc cgaacggttg
    23461 caccccaacc accgcgccca ccatattacg gctaacgtac agattaccga catgcgccga
    23521 gccagtgacc tgggcgatgg tttcatcaat gcgcgtatgg acgccaagcg tcagaccata
    23581 accggaagcg ttaatctgct cgatcagcgc cggcagctgg ttacggttgt aacgcaccac
    23641 atgcagcacc ggaccaaaga cctctttttg taattcggca aagtcatcca gttcgatcag
    23701 cgtcggagcg acaaaggtgc cgctttgcca ttcacgggca tcttcactgt tttcccgcac
    23761 tgcctggaac accgggcggc ctttgctacg catagtctga atatggcgct caatattggt
    23821 cttcgcttcg ccatcaatca ccggaccgat atcggtggtc aggcgacccg gattacccat
    23881 ccggcattcg gccattgcgc cgcgcagcat ttttaacgtg tggtcggcaa tttcatcttg
    23941 caggcacagc acgcgcagtg ccgaacaacg ctgacctgca ctgtcgaacg ccgaggccag
    24001 cacatctatt acgacttgtt cggtcagtgc tgaagaatcg acaatcatcg cgttcatgcc
    24061 gccggtttcc gcaatgagcg gaattgggcg accctgagcg tccaggcggc tggcgatatt
    24121 gcgttgcagt aacgtagcga cttcggttga accggtaaac atcaccccgc gcacgcgttc
    24181 atcacccgtc agttgcgcgc ccacggtttc accccgacct ggcagcaatt gcaccacgcc
    24241 tggcggtaca cccgcttcca gcaaaatggc gatcccttgc gcggcaatca gcggcgtttg
    24301 ttctgccggt tttgccagca cgctgttacc tgccgccagt gcggcagcga tctgcccggt
    24361 gaaaatagcc agcgggaagt tccacggact gatacatacc acaggcccta atggacggtg
    24421 ggtttcgtta gcgaaatcat cccgcacctg tccggcatag tagtggagaa aatcgaccgc
    24481 ttcgcgcact tcggcaatgg cgttactgaa ggtttttccg gcttcacgca ccagaatacc
    24541 aatcagttgc tgcatctggc tttccattag cacggcagcg cgatgcaaaa tcgccgcgcg
    24601 ttcagccgga ggagtggcaa accagattgg cgcgttatta accgcacttt ccagcgcctg
    24661 ttctacttca cgcggcgtgg cttcacgcac atagcccaca atatctttcg gttccgcagg
    24721 gttaataacg ggcgacatct caccagccgc gaccgattgt tccagcattg gcaaggcctg
    24781 ccatttttgc agtgcactat tgagcagggc agaggagagc gaggccagac ggtgttcgtt
    24841 agcgagatcc agccctgccg agttgtcgcg cccgtgaccg taaagatcgc gcggcagggg
    24901 aattttcgga tgcggtaatc cagtttgccc ttcctgttgc gccagttttt ctacagcagt
    24961 gactggatcg gcgaccagtt catccagtgg caaagaggtg tcggcaatac ggttaacaaa
    25021 cgaggtgtta gcaccgtttt ccagcaggcg gcgcaccaga tatgccagca gcgtttcatg
    25081 tgtaccgacc ggagcataaa tacgacacgg acggttaagt ttgccgtcgg caactttccc
    25141 ggtgacctgc tcatacagta gctcgcccat accatgcagg cactggaact cgtactgacc
    25201 cgggtagtag ttttgacccg ccagttgata aattgccgcc agcgtatggg cgttgtgcgt
    25261 cgcgaactgc gggtagatta gatttggcac cgccagcagt tttttcgcac aggcaagata
    25321 agaaacgtcg gtatacacct tgcgggtata aaccggataa ccttcaaggc cgtccatctg
    25381 tgcacgcttg atttcactat cccagtacgc gcctttcacc aggcgaatca tcagacggcg
    25441 acggctgcgg gtggcgagat caatcaggta atcgatcacc aacgggcagc gtttttgata
    25501 agcctgaata acaaaaccga tgccgttcca gcctgccagt tcaggctcga aacagagttt
    25561 ttccagcaga tcgagggaga tctccagacg atcggcctct tcggcatcga tgttaatacc
    25621 aatatcgtac tgacgcgcca gcagggtgag tgatttcaga cgcgggtaaa gctcttccat
    25681 tacccggtca tactgggcgc ggctgtaacg cggatgcagc gccgacagct tgattgaaat
    25741 gcccggacct tcatagatac cacgaccgtt agacgcttta ccaatggcgt gaatcgcctg
    25801 ctgataggaa accatatacg cctgtgcgtc tgcggcagtc agtgcagctt cgcccagcat
    25861 atcgtaagag taacggaaac ctttctcttc cagcttgcgg gcattggcta acgcttccgc
    25921 gatggtttcg ccagtgacga actgctcgcc catcaggcgc atcgccatat ccacaccttt
    25981 gcggatcagc ggttcaccgc ttttaccgat aatgcggttc agcgagcggg agaggctggc
    26041 ttcgttatgg gtggaaacca gtttgccggt aaacagcagc ccccaggtag cggcattaac
    26101 aaacagggac gggctacgac caatgtgtga ctgccagtta ccgttactga ttttgtcgcg
    26161 aattaacgcg tcacgggtgg ctttgtcggg aatacgcaac aacgcttccg ccagacacat
    26221 cagcgccacg ccttcctgcg atgacaacga aaactcctgc aataacccct gaaccatacc
    26281 tgcgcgacca ctggcgtttt tttgattacg cagtttatcg gccagttgat acgccagttt
    26341 gtgcgcctgt tcagctactg gctgcggcag gcgagcttgt tccagcagca tagaaaccgc
    26401 ttcggtttcc gggcggcgat aggcggcggt gatggcggcg cgggaaaccg actggggcaa
    26461 tatttgctcg gcaaagtcga ggaaaggctg gtgtggttcc tctgccggag tcggtgcttc
    26521 atcgctctca ttggccgcgc cagaaagcag cgcaggtagc tccgggagag tatcgctgtt
    26581 ttccagttgt tcaagataag aaaaaatcgc ctgtttaatt aaccagtgtg gtgtgcgatc
    26641 gatacgtgtc gcggcagact taatacgctc acgcgtcgcg tcgtccagct taacccccat
    26701 ggtggtggtt cccatgccat tactcctgtt gttctgaaag gtgcaactta acgttatcgt
    26761 gaaatatcca tgatgttgca actttgtgca accgtgttaa atgtgacctg cgtagcaagc
    26821 ttaaaaatga atgaaatgtt aataaacgaa atcgatatga cagggattaa aaaaataact
    26881 cagacttttt ctctgcggca gttaacattt ttgaaaggtg caaccgcaaa aaatgtgaga
    26941 gagtgcaacc tgatgaaaaa tagtgtcgct gagcactaaa atttaatgta aatggtgtgt
    27001 taaatcgatt gtgaataacc agcgcttccg gcaggatacg gtcgccctgg taaaacataa
    27061 actctgttac cccgttccgg tggcagatat aacggcaagt ttcgacattg ccgataataa
    27121 ttttttggag actttagatg gctattagca caccgatgtt ggtgacattt tgtgtctata
    27181 tctttggcat gatattgatt gggtttatcg cctggcgatc aacgaaaaac ttcgacgact
    27241 atattctggg cggtcgtagt cttgggccat tcgtgacggc attatcggcg ggtgcgtcgg
    27301 atatgagcgg ctggctgtta atggggttgc caggcgctgt ttttctttcc gggatttccg
    27361 aaagctggat cgccattggt ctgacattag gcgcgtggat caactggaaa ctggtggccg
    27421 ggcggttgcg tgtgcatacc gaatacaaca ataacgcctt aacattgccg gattatttca
    27481 ccgggcgctt tgaagataaa agccgcattt tgcgcattat ctccgcgctg gttattttgc
    27541 tgttcttcac catttattgc gcttcgggca ttgtggcagg cgcgcgtctg tttgaaagta
    27601 cctttggcat gagctacgaa acggctctgt gggcgggggc tgcggcgacg atcctttaca
    27661 cctttattgg cggtttcctc gcggtgagct ggactgacac tgtacaggcc agtctgatga
    27721 tttttgccct gatcctgacg ccagttatcg tcattatcag tgtcggtggc tttggtgact
    27781 cgctggaagt aatcaaacaa aagagcattg aaaacgtgga tatgctcaaa gggttgaatt
    27841 ttgtcgccat tatctcgctg atgggctggg ggctgggtta cttcgggcag ccgcacatcc
    27901 tggcgcgttt tatggcggca gattctcacc acagcattgt ccatgcgcgt cgtattagta
    27961 tgacctggat gatcctctgc ctggcagggg cggtggctgt cggcttcttt ggtatcgctt
    28021 actttaacga gcacccggcg gtagcaggtg cggtaaacca gaacgccgag cgcgtgttta
    28081 ttgaactggc acaaattctg tttaacccgt ggattgccgg gattttgctg tcggcgattc
    28141 tggcggcggt aatgtcaacg ttaagttgcc agctgctggt atgttccagt gcgattaccg
    28201 aagatttata caaagcgttt ctgcgtaaac aggccagcca gaaagagctg gtgtgggtag
    28261 ggcgagtgat ggtgctggtg gtggcgctgg tggcgattgc gctggcggca aacccggaaa
    28321 accgcgtgct gggcttagtg agctacgcgt gggcaggctt tggcgcagcg tttggtccgg
    28381 tggtgctgtt ctcggtgatg tggtcacgca tgacgcgcaa cggtgcgctg gcggggatga
    28441 tcatcggtgc gctgacggtt atcgtctgga aacagttcgg ctggctgggt ctgtacgaaa
    28501 ttattccggg ctttatcttc ggcagtatcg ggattgtagt gtttagtttg ctgggtaaag
    28561 cgccgtcagc ggcgatgcaa aaacgctttg ccgaggccga tgcgcactat cattcggctc
    28621 cgccgtcacg gttgcaggaa gggtaaggga aggcgtttta ccggaaattt cacgtcataa
    28681 caggaagcag gcagagggca atcagtgatt gccctccgcg ttttttctca aaatattttt
    28741 acatcttctc atcattatca ctacattcca cttgtatttt tcttcatcgt aatgataatc
    28801 gctatcactg cgatttactt ttctttgcat agattgactc agaaaaacgt ttaagggtgg
    28861 gtggcatgtt tgttccgttt ctcattatgt tgcgcgaagg acttgaagcc gcgctgattg
    28921 tcagtttgat tgccagctat cttaagcgta cccagcgagg ccgatggatt ggtgtgatgt
    28981 ggattggcgt gctgtttgcc gctgcgttgt gcctgggctt gggtatcttc attaacgaaa
    29041 ccaccggcga atttccgcaa aaagaacagg aactgtttga aggtatcgtg gcggtgatcg
    29101 ccgtggtgat ccttacctgg atggttttct ggatgcgcaa agtgtcgcgc aacgtcaaag
    29161 tgcaactgga acaggcagtc gatagcgcat tgcagcgtgg aaatcatcat ggctgggcgc
    29221 tggtgatgat ggtctttttt gccgttgcaa gggaagggct ggagtcggtc tttttcctgc
    29281 tggcggcgtt tcaacaagat gtcgggatct ggccgccgct gggtgcaatg ctcggtcttg
    29341 ctacagccgt ggtgctcggc ttcctgctct actggggagg tattcgcctc aatcttggtg
    29401 cattttttaa atggaccagc ctgtttattc tcttcgtcgc cgcagggctg gcggctggtg
    29461 ccattcgcgc atttcatgaa gccggattgt ggaaccactt tcaggaaatc gccttcgata
    29521 tgagtgcggt gctctcaact cattcgctgt ttggcacgct gatggaaggg atttttggct
    29581 atcaggaagc gccgagcgtc agcgaagtcg ccgtctggtt tatttatctc atcccggcgc
    29641 tggtggcatt tgctctgcca ccacgcgcag gggcgacagc gtctcgctcc gcgtaacaaa
    29701 tacgacgcaa actcttgctt agttacaaca tactttaaag ggatagtctc gtcatgacca
    29761 ttaacttccg ccgtaacgca ttgcagttga gcgtggctgc gctgttttct tctgctttta
    29821 tggctaacgc cgctgatgtg ccgcaggtca aagtgaccgt gacggataag cagtgcgaac
    29881 cgatgaccat tacggttaac gccgggaaaa cacagttcat tattcagaac cacagccaga
    29941 aggcgctgga gtgggagatc ctcaaaggcg tgatggtggt ggaagagcgg gaaaatatcg
    30001 cccctggttt tagccagaaa atgacggcaa atttacagcc tggtgaatac gatatgacct
    30061 gcggtctgct gacaaacccg aaagggaagt tgatcgtcaa aggtgaagta acggcggatg
    30121 cggcgcaaag tgatgcactt ttgagtcttg gtggtgcaat tactgcatat aaagcgtatg
    30181 tcatggcgga aaccacgcag ctggtgaccg acaccaaagc atttaccgac gctattaaag
    30241 caggcgatat cgaaaaagcg aaagcactgt atgcgccgac gcgccagcac tatgagcgca
    30301 ttgaaccgat tgctgaactg ttctccgatc tggatggcag cattgacgcc cgtgaagatg
    30361 attacgagca aaaagccgcc gatccgaaat tcaccggttt ccaccgtctg gaaaaagcat
    30421 tgtttggcga taacaccacc aaagggatgg ataagtatgc cgaccagctt tataccgatg
    30481 tggtcgattt gcaaaaacgc atcagtgaac tggctttccc tccttcaaaa gtggtcggcg
    30541 gtgcagccgg actgattgag gaagtggcgg ccagcaaaat cagcggtgaa gaagatcgct
    30601 acagccacac cgatctgtgg gatttccagg ctaacgttga aggctcgcag aaaattgtcg
    30661 atctgctgcg tccacaacta caaaaagtga atccggaact gttggcaaaa gtcgatgcca
    30721 acttcataaa ggtcgatacc attctggcga aataccgtac taaagacggt tttgaaaatt
    30781 acgacaaact gaccgatgct gaccggaatg cactgaaagg accgattact gcgctggcgg
    30841 aagatctggc gcaacttcgc ggtgtgctgg ggctggacta agcgttatgc agtatgaaga
    30901 tgaaaacggc gtgaatgaac cgtcacgccg acgtttactg aaagggattg gtgcgctggc
    30961 gctggcggga agttgtccgg tcgctcatgc acaaaaaacg caaagtgcgc cgggtacgct
    31021 ttcaccggat gcacgcaatg agaaacagcc gttttatggt gagcatcagg cagggatcct
    31081 gacaccacaa caggctgcaa tgatgctggt ggcgtttgat gtgcttgcca gcgataaagc
    31141 cgatcttgaa cggttgtttc gcttgttgac tcagcgtttt gcttttctga ctcagggcgg
    31201 agcggcacca gaaacgccaa atccgcgcct gccaccactc gattccggca ttcttggcgg
    31261 ctacattgcg cccgataatc tcaccatcac gttatcggtg ggtcactcat tgtttgatga
    31321 gcgctttggc cttgcgccac agatgccaaa aaagttgcag aagatgacgc gtttccctaa
    31381 cgactcgctg gatgcggcgt tatgtcatgg tgatgtgttg ctacaaattt gcgccaacac
    31441 ccaggacacg gtgatccatg cgctgcgcga tatcatcaaa cacacgccgg atttgctcag
    31501 cgtgcgctgg aagcgggaag ggtttatttc cgaccacgca gcgcgtagta aaggcaaaga
    31561 gacgccgata aatttgctgg gctttaaaga cggcaccgcc aatcccgata gccagaatgc
    31621 gaagttgatg caaaaagtgg tgtgggtgac ggcagatcag caggagcctg cgtggacaat
    31681 cggtggcagc tatcaggcag tacgcttgat tcagtttcga gtggaatttt gggacagaac
    31741 gccgctgaaa gagcagcaga cgatatttgg tcgtgataag caaaccggtg cgccgctggg
    31801 catgctgcat gagcatgatg ttcccgatta cgctagcgat ccggaaggga aggtgattgc
    31861 gctggacagc catatccggc tggcgaatcc ccgcacggcg gagagcgagt ccagcctgat
    31921 gctgcgtcgt ggctacagtt attcgttggg cgtcaccaat tccgggcaac tcgatatggg
    31981 gctactgttt gtctgctacc aacacgatct ggaaaagggc ttcctgacag tacaaaaaag
    32041 gttgaatgga gaggcgctgg aggaatacgt taaacccatc ggcggcggtt atttttttgc
    32101 gctgccggga gtaaaggacg cgaatgatta tctcggaagc gcattattgc gggtttaatg
    32161 tttttaggcg gataaggcgt ttacgccgca ctaggcgttc gtgcgctgat gcgacgcttg
    32221 cgcgtcttat catgcctaca atcggtgcgg gtcggtaggt tggataaggc gttcaagccg
    32281 catccggcaa gcttttacca gtactgcgaa tcctgctgaa agcacacagc ttttttcatc
    32341 actgtcatca ctctgtcatc tttccggtag aaactaatgt cactgaaatg gtgttttatg
    32401 gttaaatata agtgaatata ttgttgcaat gaatgcgaaa tctgttgtac ttattaagta
    32461 gcagcggaag ttcccggcag tgatagtcag tcactatgga gatcgcggat ggtaacgtcc
    32521 tgtactggac atgttttaga taatcagcgc gccaccactc gcggagattt ctcctccggt
    32581 agtcatctcg tcactttgta ttttcaacct catcctttct tttcatgtgt taccgacgcc
    32641 gtaaacggtg cgcgtagccg tttttcggca ttttatccaa aagcaaacta tggcttacaa
    32701 ggaagccaac cctctgatgt tcgtgcgcat aatcgcgctg ccaacggcgc gtgtgatgaa
    32761 tacaaacaac tcaaggtgct ctccatggga agacaaaaag cagtgatcaa agctcgtcgc
    32821 gaggcaaaac gtgtgctgag acgggattcg cgcagccata agcagcgtga agaagaatcg
    32881 gtcacctcgc ttgtgcagat gagcggcgta gaagccattg gtatggcccg cgacagtcgc
    32941 gatacctcgc ccatcctcgc gcgaaatgaa gcgcaattgc actatctgaa ggctattgag
    33001 agtaagcagc tgatatttgc cacgggcgaa gccgggtgcg gaaaaacctg gatcagcgca
    33061 gcaaaagcgg cagaggccct gatacataag gatgtcgaca ggattatcgt cacccgtcca
    33121 gttctgcaag ctgatgaaga tcttggcttc ttacctggtg atatcgcaga aaagtttgct
    33181 ccctattttc gcccggtcta cgacgtgctg gtccgtcgct taggggcttc ctttatgcag
    33241 tactgcctgc gaccggaaat agggaaggtg gaaattgcgc cgttcgccta tatgcgtgga
    33301 cgtacctttg aaaatgcagt cgtcattctt gacgaggcgc agaatgtgac tgccgcgcaa
    33361 atgaaaatgt ttttaacccg cctcggggag aacgtgacgg ttatcgtcaa cggtgatatc
    33421 acgcaatgcg atttgcctcg cggcgtgcgc tccggattaa gtgacgcgct ggaacgtttc
    33481 gaagaggatg aaatggtcgg gatcgtccga ttcggtaaag aggattgcgt acgttcggca
    33541 ctttgccaac gtacgctaca tgcatacagt taagtgtatt atcggtgcag agcccgggcg
    33601 aaccgggctt tgttttattt cattaacagc aaagattttc acttcaggtt aacgttcagg
    33661 gggcgacttt gagcgtgttg ataagctgaa atgaattcag gtttagatac tcttactggg
    33721 catttgtcgt catagcacca gaccagtgta ataccgccag tgcggagagt ttttctttca
    33781 gaagcccaaa aagcgagcat ctgcctatct tttttcagcc attcaacctg tacaggaggt
    33841 ctggtgccaa ttttgcgtat gaggttccat caataatgga ccataatttg actgactgat
    33901 taacaccgaa tacattctcc t
//
LOCUS       DADSYM010000036        33649 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2415
            SAMN08039579-rid11723173.denovo.036, whole genome shotgun sequence.
ACCESSION   DADSYM010000036 DADSYM010000000
VERSION     DADSYM010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039579
            Sequence Read Archive: SRR6362533
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 33649)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33649)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980162.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 03:39:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,484
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 4,347
            CDSs (with protein)               :: 4,347
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 43 of 85
            Pseudo Genes (incomplete)         :: 37 of 85
            Pseudo Genes (internal stop)      :: 19 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33649
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039579-rid11723173.denovo.036"
                     /strain="LGE2415"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8302 N 95.9628 W"
                     /collection_date="2015-08-24"
     gene            23..259
                     /locus_tag="J1Y83_001437"
     CDS             23..259
                     /locus_tag="J1Y83_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1656 domain-containing protein"
                     /protein_id="HBA7629596.1"
                     /translation="MKFMLNATGLPLQDLVFGASVYFPPFFKAFAFGFVIWLVVHRLL
                     RGWIYAGDIWHPLLMDLSLFAICVCLALAILIAW"
     gene            262..1119
                     /locus_tag="J1Y83_001438"
     CDS             262..1119
                     /locus_tag="J1Y83_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416161.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HBA7629597.1"
                     /translation="MSIKTIKYFSTIIVAVVAVLAGWWLWNYYMQSPWTRDGKIRAEQ
                     VSITPQVSGRIVELNIKDNQLVNAGDLLLMIDKTPFQIAELNAQAQLAKAQSDLAKAN
                     NEANRRRHLSQNFISAEELDTANLNVKAMQASVDAAQATLKQAQWQLAQTEIRAPVSG
                     WVTNLTTRIGDYADTGKPLFALVDSHSFYVIGYFEETKLRHIREGAPAQITLYSDNKT
                     LQGHASSIGRAIYDQSVESDSSLIPDVKPNVPWVRLAQRVPVRFTLDKVPGDVTLVSG
                     TTCSIAVGQ"
     gene            1119..3131
                     /locus_tag="J1Y83_001439"
     CDS             1119..3131
                     /locus_tag="J1Y83_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HBA7629598.1"
                     /translation="MNASSWSLRNLPWFRATLAQWRYALRNTIAMCLALTVAYYLNLD
                     EPYWAMTSAAVVSFPTVGGVISKSLGRIAGSLLGAIAALLLAGHTLNEPWFFLLSMSA
                     WLGFCTWACAHFTNNVAYAFQLAGYTAAIIAFPMVNITEASQLWDIAQARVCEVIVGI
                     LCGGMMMMILPSSSDATALLTALKNMHARLLEHASLLWRPETTDAIRAAHEGVIGQIL
                     TMNLLRIQAFWSHYRFRQQNARLNALLHQQLRMTSVISSLRRMLLNWPSPPDATREIL
                     EQLLTALASSQTDVYTVARIISPLRPTNVADYRHVAFWQRLRYFCRLYLQSSQELHRL
                     QSGVDDHARLPRTSGLARHTDNAEAMWSGLRTFCTLMMIGAWSIASQWDAGANALTLA
                     AISCVLYSAVAAPFKSLSLLMRTLVLLSLFSFVVKFGLMVQISDLWQFLLFLFPLLAT
                     MQLLKLQMSKFAALWGQLIVFMGSFIAVTNPPVYDFADFLNDNLAKIVGVALAWLAFA
                     ILRPGSDARKSRRHIRALRRDFVDQLSRHPTLSESEFESLTYHHVSQLSNSQDALARR
                     WLLRWGVVLLNCSHVVWQLRDWESRSDPLSRVRDNCISLLRGVMSERGVQQKSLAATL
                     EELQRICDSLARHHQPAARELAAIVWRLYCSLSQLEQAPPQGTLAS"
     gene            complement(3132..3653)
                     /gene="sodC"
                     /locus_tag="J1Y83_001440"
     CDS             complement(3132..3653)
                     /gene="sodC"
                     /locus_tag="J1Y83_001440"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Cu-Zn] SodC2"
                     /protein_id="HBA7629599.1"
                     /translation="MKRFSLAILALVVATGAQAASEKVEMNLVTSQGVGQSIGSVTIT
                     ETDKGLEFSPDLKALPPGEHGFHIHAKGSCQPATKDGKASAAESAGGHLDPQNTGKHE
                     GPEGAGHLGDLPALVVNNDGKATDAVIAPRLKSLDEVKDKALMVHVGGDNMSDQPKPL
                     GGGGERYACGVIK"
     gene            complement(3734..4630)
                     /locus_tag="J1Y83_001441"
     CDS             complement(3734..4630)
                     /locus_tag="J1Y83_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase family oxidoreductase"
                     /protein_id="HBA7629600.1"
                     /translation="MVQRITIAPQGPEFSRFVMGYWRLMDWNMSARQLVSFIEEHLDL
                     GVTTVDHADIYGGYQCEAAFGEALKLAPHLRERMEIVSKCGIATTACEENVIGHYITD
                     RDHIIKSAEQSLINLATDHLDLLLIHRPDPLMDADEVADAFKHLHQSGKVRHFGVSNF
                     TPAQFALLQSRLPFTLATNQVEISPVHQPLLLDGTLDQLQQLRIRPMAWSCLGGGRLF
                     NDDYFQPLRDELAVVAEELNAGSIEQVVYAWVLRLPSQPLPIIGSGKIERVRAAVEAE
                     TLKMTRQQWFRIRKAALGYDVP"
     gene            complement(4679..4918)
                     /locus_tag="J1Y83_001442"
     CDS             complement(4679..4918)
                     /locus_tag="J1Y83_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1289 domain-containing protein"
                     /protein_id="HBA7629601.1"
                     /translation="MAEQLEFFPVQSPCRGICQSDERGFCRGCFRSRDERFNWNKMSD
                     GEKQEVLRLCRQRLMRKLRVNKPAPSDEPEQPSLF"
     gene            5021..5620
                     /locus_tag="J1Y83_001443"
     CDS             5021..5620
                     /locus_tag="J1Y83_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416166.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBA7629602.1"
                     /translation="MNKHTEHDTREHLLATGEQLCLQRGFTGMGLSELLKTAEVPKGS
                     FYHYFRSKEAFGVAMLERHYAAYHQRLTELLQSGEGNYRDRILAYYQQTLNQFCQHGT
                     ISGCLTVKLSAEVCDLSEDMRSAMDKGARGVIALLSQALENGRENHCLTFCGEPLQQA
                     QVLYALWLGANLQAKISRSFEPLENALAHVKNIIATPAV"
     gene            5657..6754
                     /gene="nemA"
                     /locus_tag="J1Y83_001444"
     CDS             5657..6754
                     /gene="nemA"
                     /locus_tag="J1Y83_001444"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-ethylmaleimide reductase"
                     /protein_id="HBA7629603.1"
                     /translation="MSSEKLYSPLKVGAITAANRIFMAPLTRLRSIEPGDIPTPLMAE
                     YYRQRASAGLIISEATQISAQAKGYAGAPGIHSPEQIAAWKKITAGVHAENGHMAVQL
                     WHTGRISHASLQPGGQAPVAPSALSAGTRTSLRDENGQAIRVETSIPRALELGEIPGI
                     VNDFRQAIANAREAGFDLVELHSAHGYLLHQFLSPSSNHRTDQYGGSVENRARLVLEV
                     VDAGIEEWGADRIGIRISPIGTFQNTDNGPNEEADALYLIEQLGKRGIAYLHMSEPDW
                     AGGEPYTDAFREKVRARFHGPIIGAGAYTVEKAETLIGKGLIDAVAFGRDWIANPDLV
                     ARLQRKAELNPQRAESIYGGGAEGYTDYPTL"
     gene            6835..7242
                     /gene="gloA"
                     /locus_tag="J1Y83_001445"
     CDS             6835..7242
                     /gene="gloA"
                     /locus_tag="J1Y83_001445"
                     /EC_number="4.4.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008500591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HBA7629604.1"
                     /translation="MRLLHTMLRVGDLQRSIDFYTNVLGMKLLRTSENPEYKYSLAFV
                     GYGPETEEAVIELTYNWGVDKYELGTAYGHIALSVDNAAEACEKIRQNGGNVTREAGP
                     VKGGTTVIAFVEDPDGYKIELIEEKDAGRGLGN"
     gene            7345..7992
                     /gene="rnt"
                     /locus_tag="J1Y83_001446"
     CDS             7345..7992
                     /gene="rnt"
                     /locus_tag="J1Y83_001446"
                     /EC_number="3.1.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease T"
                     /protein_id="HBA7629605.1"
                     /translation="MSDNAQLTGLCDRFRGFYPVVIDVETAGFNAKTDALLEIAAITL
                     KMDEQGWLMPDTTLHFHVEPFVGANLQPEALAFNGIDPNDPDRGAVSEYEALHEIFKV
                     VRKGIKASGCNRAIMVAHNANFDHSFMMAAAERASLKRNPFHPFATFDTAALAGLALG
                     QTVLSKACQTAGMDFDSTQAHSALYDTERTAVLFCEIVNRWKRLGGWPLPAAAEV"
     gene            8085..12701
                     /locus_tag="J1Y83_001447"
     CDS             8085..12701
                     /locus_tag="J1Y83_001447"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001361457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HBA7629606.1"
                     /translation="MANNPNPSSPLPDVFSPATRDWFLRAFKQPTAVQSQTWHVAARG
                     EHALVIAPTGSGKTLAAFLYALDQLFREGGEDTREAHKRKTSRILYISPIKALGTDVQ
                     RNLQIPLQGIADERRRRGETEVNLRVGIRTGDTPAQERSKLTRNPPDILITTPESLYL
                     MLTSRARETLRGVETVIIDEVHAVAGSKRGAHLALSLERLDALLHTSAQRIGLSATVR
                     SASDVAAFLGGDRPVTVVNPPAMRHPQIRIVVPVANMNDVSSVASGTGEDSHAGREGS
                     IWPYIETGILDEVLRHRSTIIFTNSRGLAEKLTARLNELYAARLQRSPSIAVDAAHFE
                     STSGATSNRVQSSDIFIARSHHGSVSKEQRAITEQALKSGELRCVVATSSLELGIDMG
                     AVDLVIQVATPLSVASGLQRIGRAGHQVGGVSKGLFFPRTRRDLVDSAVIVECMFAGR
                     LENLTPPHNPLDVLAQQTVAAAAMDALQVDEWYSCVRRAAPWKDLPGRVFDATLDMLS
                     GRYPSGDFSAFRPKLVWNRETGILTARPGAQLLAVTSGRTIPDRGMYSVLLPEGEEQA
                     GSRRVGELDEEMVYESRVNDIITLGATSWRIQQITRDQVIVTPAPGRSARLPFWRGEG
                     NGRPAELGEMIGDFLHLLADGEFFSGTIPPWLAEENTIANIQGLIDEQRNATGIVPGS
                     RHLVLERCRDEIGDWRIILHSPYGRRVHEPWALAIAGRIHALWGADASVVASDDGIVA
                     RIPDTDGKLPDAAIFLFEPEKLLQIVREAVGSSALFAARFRECAARALLMPGRTPGHR
                     TPLWQQRLRASQLLEIAQGYPDFPVILETLRECLQDVYDLPALERLMRRLNGGEIQIS
                     DVTTTTPSPFAASLLFGYVAEFMYQSDAPLAERRASVLSLDSELLRNLLGQVDPGELL
                     DPQVIRQVEEELQRLAPGRRAKGEEGLFDLLRELAPMTVEDLAQRHTGSSEDIASYLE
                     NLLAVKRIFPTMISGQERLACMDDAARLRDALGVRLPESLPEIYLHRVSYPLRDLFLR
                     YLRAHALVTAEQLAHEFSLGIAIVEEQLQQLREQGLVMNLQQDIWVSDEVFRRLRLRS
                     LQAAREATRPVAATTYARLLLERQGVLPATDGSPALFASTSPGVYEGVDGVMRVIEQL
                     AGVGLPASLWESQILPARVRDYSPEMLDELLATGAVIWSGQKKLGEDDGLVALHLQEY
                     AAESFTPAEADQANRSALQQAIIAVLADGGAWFAQQISQRIRDKIGESVDPSALQEAL
                     WALVWQGVITSDIWAPLRALTRSSSNARTSTRRSHRARRGRPVYAQPVSPLVSYNTPK
                     LAGRWSLLQVEPLNDTERMLALAENMLDRYGIISRQAVIAENIPGGFPSMQTLCRSME
                     DSGRIMRGRFVEGLGGAQFAERLTIDRLRDLATQTAQTRHYTPVALSANDPANVWGNL
                     LPWPAHPATLVPTRRAGALVVVSGGKLLLYLAQGGKKMLVWQEKEELLAPEVFHALTT
                     ALRREPRLRFTLTEVNDLPVRQTPMFTLLREAGFSSSPQGLDWE"
     gene            complement(12752..13099)
                     /gene="grxD"
                     /locus_tag="J1Y83_001448"
     CDS             complement(12752..13099)
                     /gene="grxD"
                     /locus_tag="J1Y83_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monothiol glutaredoxin 4"
                     /protein_id="HBA7629607.1"
                     /translation="MSTTIEKIQRQIAENPILLYMKGSPKLPSCGFSAQAVQALAACG
                     ERFAYVDILQNPDIRAELPKYANWPTFPQLWVDGELVGGCDIVIEMYQRGELQQLIKE
                     TAAKYKSEEPDAE"
     gene            13434..14261
                     /locus_tag="J1Y83_001449"
     CDS             13434..14261
                     /locus_tag="J1Y83_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase"
                     /protein_id="HBA7629608.1"
                     /translation="MARINRISITLCALLFTTLPLTPMAHASKQARESSATTHITKKA
                     DKKKSTATTKKTQKASQKTAKKAASKSTTKSKTASSVKKSSITASKNAKTRSKHAVNK
                     TASASFTEKCTKRKGYKSHCVKVKNAASGTLADAHKAKVQKATKVAMNKLMQQIGKPY
                     RWGGSSPRTGFDCSGLVYYAYKDLVKIRIPRTANEMYHLRDAAPIERSELKNGDLVFF
                     RTQGRGTADHVGVYVGNGKFIQSPRTGQEIQITSLSEDYWQRHYVGARRVMTPKTLR"
     gene            14389..14970
                     /gene="sodB"
                     /locus_tag="J1Y83_001450"
     CDS             14389..14970
                     /gene="sodB"
                     /locus_tag="J1Y83_001450"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020079002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Fe]"
                     /protein_id="HBA7629609.1"
                     /translation="MSFELPALPYAKDALAPHISAETIEYHYGKHHQTYVTNLNNLIK
                     GTAFEGKSLEEIIRSSEGGVFNNAAQVWNHTFYWNCLAPNAGGEPTGKVAEAIAASFG
                     SFADFKAQFTDAAIKNFGSGWTWLVKNSDGKLAIVSTSNAGTPLTTDATPLLTVDVWE
                     HAYYIDYRNARPGYLEHFWALVNWEFVAKNLAA"
     gene            complement(15116..16285)
                     /locus_tag="J1Y83_001451"
     CDS             complement(15116..16285)
                     /locus_tag="J1Y83_001451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBA7629610.1"
                     /translation="MKINYPLLALAIGAFGIGTTEFSPMGLLPVIARGVDVSIPAAGM
                     LISAYAVGVMVGAPLMTLLLSHRARRSALIFLMAIFTLGNVLSAIAPDYMTLMLSRIL
                     TSLNHGAFFGLGSVVAASVVPKHKQASAVATMFMGLTLANIGGVPAATWLGETIGWRM
                     SFLATAGLGVISMVSLFFSLPKGGAGARPEVKKELAVLMRPQVLSALLTTVLGAGAMF
                     TLYTYISPVLQSITHVTPVFVTAMLVLIGVGFSIGNYLGGKLADRSVNGTLKGFLLLL
                     MVIMLAIPFLARNEFGAAISMVVWGAATFAVVPPLQMRVMRVASEAPGLSSSVNIGAF
                     NLGNALGAAAGGAVISAGLGYSFVPVMGAIVAGLALLLVFMSARKQPETVCVANS"
     gene            complement(16451..16540)
                     /gene="ynhF"
                     /locus_tag="J1Y83_001452"
     CDS             complement(16451..16540)
                     /gene="ynhF"
                     /locus_tag="J1Y83_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein YnhF"
                     /protein_id="HBA7629611.1"
                     /translation="MSTDLKFSLVTTIIVLGLIVAVGLTAALH"
     gene            16839..17864
                     /gene="purR"
                     /locus_tag="J1Y83_001453"
     CDS             16839..17864
                     /gene="purR"
                     /locus_tag="J1Y83_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor PurR"
                     /protein_id="HBA7629612.1"
                     /translation="MATIKDVAKRANVSTTTVSHVINKTRFVAEETRNAVWAAIKELH
                     YSPSAVARSLKVNHTKSIGLLATSSEAAYFAEIIEAVEKNCFQKGYTLILGNAWNNLE
                     KQRAYLSMMAQKRVDGLLVMCSEYPEPLLAMLEEYRHIPMVVMDWGEAKADFTDAVID
                     NAFEGGYMAGRYLIERGHREIGVIPGPLERNTGAGRLAGFMKAMEEAMIKVPESWIVQ
                     GDFEPESGYRAMQQILSQSHRPTAVFCGGDIMAMGALCAADEMGLRVPQDVSLIGYDN
                     VRNARYFTPALTTIHQPKDSLGETAFNMLLDRIVNKREEPQSIEVHPRLIERRSVADG
                     PFRDYRR"
     gene            complement(17861..18793)
                     /locus_tag="J1Y83_001454"
     CDS             complement(17861..18793)
                     /locus_tag="J1Y83_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBA7629613.1"
                     /translation="MWSEYSLEVVDAVARNGSFSAAAQELHRVPSAVSYTVRQLEEWL
                     AVPLFERRHRDVELTAAGAWFLKEGRSVVKKMQITRQQCQQIANGWRGQLAIAVDNIV
                     RPERTRQMIVDFYRHFDDVELLVFQEVFNGVWDALSDGRVELAIGATRAIPVGGRYAF
                     RDMGMLSWSCVVASHHPLALMDGPFSDDTLRNWPSLVREDTSRTLPKRITWLLDNQKR
                     LVVPDWESSATCISAGLCIGMVPTHFAKPWLNEGKWVALELENPFPDSACCLTWQQND
                     MSPALTWLLEYLGDSETLNKEWLREPEETPATGN"
     gene            18906..20117
                     /locus_tag="J1Y83_001455"
     CDS             18906..20117
                     /locus_tag="J1Y83_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bcr/CflA family multidrug efflux MFS
                     transporter"
                     /protein_id="HBA7629614.1"
                     /translation="MQPGKRFLVWLAGLSVLGFLATDMYLPAFAAIQADLQTPASAVS
                     ASLSLFLAGFAAAQLLWGPLSDRYGRKPVLFIGLTIFALGSLGMLWVENAATLLILRF
                     VQAVGVCAAAVIWQALVTDYYPSQKVNRIFATIMPLVGLSPALAPLLGSWLLVHFSWQ
                     AIFATLFAITVVLILPIFWLKPTTKARNNSQDGLTFTDLLRSKTYRGNVLIYAACSAS
                     FFAWLTGSPFILSEMDYSPAVIGLSYVPQTIAFLIGGYGCRAALQKWQGKQLLPWLLV
                     LFAVSVIATWAAGFISHVSLVEILIPFCVMAIANGAIYPIVVAQALRPFPHATGRAAA
                     LQNTLQLGLCFLASLVVSWLISISTPLLTTTSVMLSTVVLVALGYMMQRCEEVGCQNH
                     GNAEVAHSESH"
     gene            20408..21556
                     /gene="cfa"
                     /locus_tag="J1Y83_001456"
     CDS             20408..21556
                     /gene="cfa"
                     /locus_tag="J1Y83_001456"
                     /EC_number="2.1.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclopropane fatty acyl phospholipid synthase"
                     /protein_id="HBA7629615.1"
                     /translation="MSSSCIEEVSVPDDNWYRIANELLSRAGIAINGSAPADIRVKNP
                     DFFKRVLQEGSLGLGESYMDGWWECDRLDMFFSKVLRAGLENQLPHHFKDTLRIAGAR
                     LFNLQSKKRAWIVGKEHYDLGNDLFSRMLDPFMQYSCAYWKDADNLESAQQAKLKMIC
                     EKLQLKPGMRVLDIGCGWGGLAHYMASNYDVSVVGVTISAEQQKMAQERCEGLDVTIL
                     LQDYRDLNDQFDRIVSVGMFEHVGPKNYDTYFAVVDRNLKPEGIFLLHTIGSKKTDLN
                     VDPWINKYIFPNGCLPSVRQIAQSSEPHFVMEDWHNFGADYDTTLMAWYERFLAAWPE
                     IADNYSERFKRMFTYYLNACAGAFRARDIQLWQVVFSRGVENGLRVAR"
     gene            complement(21596..22237)
                     /gene="ribE"
                     /locus_tag="J1Y83_001457"
     CDS             complement(21596..22237)
                     /gene="ribE"
                     /locus_tag="J1Y83_001457"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HBA7629616.1"
                     /translation="MFTGIVQGTAKLVSIDEKPNFRTHVVELPDHMLDGLETGASVAH
                     NGCCLTVTEINGNHVSFDLMKETLRITNLGDLKVGDWVNVERAAKFSDEIGGHLMSGH
                     IMTTAEVAKILTSENNRQIWFKVQDSQLMKYILYKGFIGIDGISLTVGEVTPTRFCVH
                     LIPETLERTTLGKKKLGARVNIEIDPQTQAVVDTVERVLAARENAMNQPGTEA"
     gene            22452..23825
                     /gene="mdtK"
                     /locus_tag="J1Y83_001458"
     CDS             22452..23825
                     /gene="mdtK"
                     /locus_tag="J1Y83_001458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MATE transporter MdtK"
                     /protein_id="HBA7629617.1"
                     /translation="MQKYISEARLLLALAIPVILAQIAQTAMGFVDTVMAGGYSATDM
                     AAVAIGTSIWLPAILFGHGLLLALTPVIAQLNGSGRRERIAHQVRQGFWLAGFVSVLI
                     MLVLWNAGYIIRSMENIDPALADKAVGYLRALLWGAPGYLFFQVARNQCEGLAKTKPG
                     MVMGFIGLLVNIPVNYIFIYGHFGMPELGGVGCGVATAAVYWVMFLAMVSYIKRARSM
                     RDIRNEKGTAKPDPAVMKRLIQLGLPIALALFFEVTLFAVVALLVSPLGIVDVAGHQI
                     ALNFSSLMFVLPMSLAAAVTIRVGYRLGQGSTLDAQTAARTGLMVGVCMATLTAIFTV
                     SLREQIALLYNDNPEVVTLAAHLMLLAAVYQISDSIQVIGSGILRGYKDTRSIFYITF
                     TAYWVLGLPSGYILALTDLVVEPMGPAGFWIGFIIGLTSAAIMMMLRMRFLQRLPSAI
                     ILQRASR"
     gene            complement(23866..25122)
                     /locus_tag="J1Y83_001459"
     CDS             complement(23866..25122)
                     /locus_tag="J1Y83_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA7629618.1"
                     /translation="MGSDAKNLMSDGNVQIVKTGEVIGATQLTEGELIVEAGGRAENT
                     VVTGAGWLKVATGGIAKCTQYGNNGTLSVSDGAIATDIVQSEGGAISLSTLATVNGRH
                     PEGEFSVDKGYACGLLLENGGNLRVLEGHRAEKIILDQEGGLLVNGTTSAVVVDEGGE
                     LLVYPGGEACNCEINQGGVFMLAGKASDTLLAGGTMNNLGGEDSDTIVENGSIYRLGT
                     DGIQLYSSGKTQNLSVNVGGRAEVHAGTLENAVIQGGTVILLSPTSADENFVVEEDRA
                     PVELTGSVALLDGASMIIGYGAELQQSTITVQQGGVLILDGSTVKGDSVTFGIGNINL
                     NGGKLWLITDAATQVQLKVKRLRGEGAICLQTSAKEISPDFINVKGEVTGDIHVEITD
                     ASRQTLCNSLKLQPDQDGIGATLQPA"
     gene            25430..25506
                     /locus_tag="J1Y83_001460"
     tRNA            25430..25506
                     /locus_tag="J1Y83_001460"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:25464..25466,aa:Val,seq:gac)
     gene            25511..25587
                     /locus_tag="J1Y83_001461"
     tRNA            25511..25587
                     /locus_tag="J1Y83_001461"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:25545..25547,aa:Val,seq:gac)
     gene            25695..26000
                     /locus_tag="J1Y83_001462"
     CDS             25695..26000
                     /locus_tag="J1Y83_001462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monooxygenase"
                     /protein_id="HBA7629619.1"
                     /translation="MATLLQLHFAFNGPFGDAMAEQLKPLAESINQEPGFLWKVWTES
                     EKNHEAGGIYLFSDEKSALTYLEKHTARLKNLGVEEVVAKVFDVNVPLSQINQAKLA"
     gene            26126..27730
                     /locus_tag="J1Y83_001463"
     CDS             26126..27730
                     /locus_tag="J1Y83_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-NAD(P)-binding protein"
                     /protein_id="HBA7629620.1"
                     /translation="MKTIAIVGAGPTGIYTLFSLLQQQTPLSISIFEQADEAGVGMPY
                     SDDENSKLMLANIASIEIPPIYCTYLEWLQKQEASHLQRYGVKKETLHDRQFLPRILL
                     GEYFRDQFLRLVDQARQQKFAVAVYESCQVTDLQITNAGVMLATNLDLPSETFDLVVI
                     ATGHVWPDEEEATRTYFPSPWSGLMEAKVDACNVGIMGTSLSGLDAAMAVAIQHGSFI
                     EDDKQHVTFHRDNASEKLNITLMSRTGILPEADFYCPIPYEPLHIITDQALNAEIQKG
                     EVGLLDRVFRLIVEEIKFADPDWSQRIALESLNVDSFAQAWFAERKQRDPFDWAEENL
                     QEVERNKREKHTVPWRYVILRLHEAVQEIVPHLNEHDHKRFSKGLARVFIDNYAAIPS
                     ESIRRLLALREAGIIHILALGEDYEMEINESRTVLKTEDNSYSFDVFIDARGQRPLKV
                     KDIPFPGLREQLQKTGDEIPDVGEDYTLQQPEDIRGRVAFGALPWLMHDQPFVQGLTA
                     CAEIGEAMARAVVKPASCARRRLSFD"
     gene            complement(27742..28554)
                     /locus_tag="J1Y83_001464"
     CDS             complement(27742..28554)
                     /locus_tag="J1Y83_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA7629621.1"
                     /translation="MIITRADLREWRIGAVMYRWFLRHFPRGGSYADIHHALIEEGYT
                     DWAESLVEYAWKKWLADENFAHQEVSSMQKLATDPGERAFCSQFARSDDHARIGCCED
                     NARIATAGYAAQIASMGYSVRIGSVGFNSHIGSSGERARVAVTGNSSRISSAGDSSRI
                     ANTGMRVRVCTLGERCHVASNGDLVQIASFGANARIANCGDNVHIIASGENSTVVSTG
                     VVDSIILGPGGCAALAYHDGERVRFAVAIEGENNIRAGVRYRLNEQHQFVEC"
     gene            complement(28558..29343)
                     /locus_tag="J1Y83_001465"
     CDS             complement(28558..29343)
                     /locus_tag="J1Y83_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate reductase cytochrome B subunit"
                     /protein_id="HBA7629622.1"
                     /translation="MNPSQHAEQFQSQLANYVPQFTPQFWPVWLIIAGLLLVGMWLVL
                     GLHALLRARGVKKSATDHGERIYLYSKAVRLWHWSNALLFVLLLASGLINHFALVGAT
                     AVKSLVAVHEVCGFLLLACWLGFVLINAVGGNGHHYRIRRQGWLERAAKQTRFYLFGI
                     MQVEEHPFPATTQSKFNPLQQVAYVGVMYGLLPLLLLTGLLCLYPQAVGDVFPGVRYW
                     LLQAHFALAFISLFFIFGHLYLCTTGRTPHETFKSMVDGYHRH"
     gene            complement(29340..30008)
                     /locus_tag="J1Y83_001466"
     CDS             complement(29340..30008)
                     /locus_tag="J1Y83_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416186.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HBA7629623.1"
                     /translation="MSFTRRKFVLGMGTVIFFTGSASSLLANTRQEKEVRYAMIHDES
                     RCNGCNICARACRKTNHVPAQGSRLSIAHIPVIDNDNGTQYHFFRQSCQHCEDAPCID
                     VCPTGASWRDEQGIVRVEKSQCIGCSYCIGACPYQVRYLNPVTKVADKCDFCAETRLA
                     KGFPPICVSACPEHALIFGHEDSPEIQAWLQENKYYQYQLPGAGKPHLYRRFGRHLIK
                     KENV"
     gene            complement(30072..30710)
                     /locus_tag="J1Y83_001467"
     CDS             complement(30072..30710)
                     /locus_tag="J1Y83_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310406.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdhW family putative oxidoreductase system
                     protein"
                     /protein_id="HBA7629624.1"
                     /translation="MNHQDELPLAEVSEIDEAKRQWLQGMRHPVDTVTEPEPAEILAE
                     FIRQHSAAGQLVARAVFLSPPYSVAEEELSVLLENIKQNGDYADIACMTGSQDDYYYS
                     TQAMSENYAAMSLQVVEQDICRAIAHAVRFECQTYPRPYKVAMLMQAPYYFQEAQIEA
                     AIATMDVAPEYADIRQVESSTAVLYLFSERFMTYGKAYGLCEWFEVEQFQNP"
     gene            complement(30723..32825)
                     /locus_tag="J1Y83_001468"
     CDS             complement(30723..32825)
                     /locus_tag="J1Y83_001468"
                     /EC_number="1.2.7.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase"
                     /protein_id="HBA7629625.1"
                     /translation="MANGWTGNILRVNLTTGNITLEDSSKFKSFVGGMGFGYKIMYDE
                     VPPGTKPFDEANKLVFATGPLTGSGAPCSFRVNITSLSTFTKGNLVVDAHMGGFFAAQ
                     MKFAGYDVIIIEGKAKSPVWLKIKDDKVSLEKADFLWGKGTRATTEEICRLTSPETCV
                     AAIGQAGENLVPLSGMLNSRNHSGGAGTGAIMGSKNLKAIAVEGTKGVNIADRQEMKR
                     LNDYMMTELIGANNNHVVPSTPQSWAEYSDPKSRWTARKGLFWGAAEGGPIETGEIPP
                     GNQNTVGFRTYKSVFDLGPAAEKYTVKMSGCHSCPIRCMTQMNIPRVKEFGVPSTGGN
                     TCVANFVHTTIFPNGPKDFEDKDDGRVIGNLVGLNLFDDYGLWCNYGQLHRDFTYCYS
                     KGVFKRVLPAEEYAEIRWDQLEAGDVNFIKDFYYRLAHRVGELSHLADGSYAIAERWN
                     LGEEYWGYAKNKLWSPFGYPVHHANEASAQVGSIVNCMFNRDCMTHTHINFIGSGLPL
                     KLQREVAKELFGSEDAYDETKNYTPINDAKIKYAKWSLLRVCLHNAVTLCNWVWPMTV
                     SPLKSRNYRGDLALEAKFFKAITGEDMTQEKLDLAAERIFTLHRAYTVKLMQTKDMRN
                     EHDLICSWVFDKDPQIPVFTEGTDKMDRDDMHASLTMFYKEMGWDPQLGCPTRETLQR
                     LGLEDIAADLAAHNLLPA"
     gene            complement(32846..33472)
                     /locus_tag="J1Y83_001469"
     CDS             complement(32846..33472)
                     /locus_tag="J1Y83_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like protein"
                     /protein_id="HBA7629626.1"
                     /translation="MNPVDRPLLNIGLTRLEFLRISGKGLAGLTIAPALLSLLGCKQE
                     DIDSGTVGLINTPKGVLVTQRARCTGCHRCEISCTNFNDGSVGTFFSRIKIHRNYFFG
                     DNGVGSGGGLYGDLNYTADTCRQCKEPQCMNVCPIGAITWQQKEGCITVDHKRCIGCS
                     ACTTACPWMMATVNTESKKSSKCVLCGECANACPTGALKIIEWKDITV"
BASE COUNT         8095 a         8865 c         8275 g         8414 t
ORIGIN      
        1 ctgtcgtaaa ggaatctact ttgtgaagtt tatgctcaat gcaacaggat tgcccttaca
       61 agatctggtg ttcggtgcgt ccgtctactt tcctccgttt ttcaaagcct tcgcgtttgg
      121 attcgtcatc tggcttgtcg tacaccgcct gcttcgtggc tggatctacg ccggtgacat
      181 ctggcatccc ttgttaatgg atttatcgct gtttgcgatt tgcgtttgcc ttgccctggc
      241 aatactgatt gcgtggtaac tatgtcaatt aaaacaatta agtatttctc aacaatcatt
      301 gtagcggtag ttgcggttct tgccggatgg tggctgtgga attattacat gcaatctccg
      361 tggacacgcg acgggaaaat acgcgcagaa caggtttcta tcaccccaca ggtgtcggga
      421 cgtattgttg agctgaatat aaaagacaac cagctggtta acgcggggga tcttctgctc
      481 atgatcgata aaacgccttt tcagatcgcc gagctgaacg cacaggctca gctggcgaaa
      541 gcgcaatctg acctcgcaaa agccaataac gaagcgaatc gtcgtcgtca tctctcacaa
      601 aattttattt ctgccgaaga actcgatacc gctaacctca atgttaaagc gatgcaggcc
      661 agcgttgatg ccgcacaggc gacgctgaaa caggcacaat ggcaactggc gcaaacggaa
      721 attcgcgccc cggtgagtgg atgggtgact aatctcacta cccgcatcgg cgactacgct
      781 gacaccggga aaccgctgtt tgcccttgtc gacagccact cgttttacgt cattggttat
      841 tttgaagaaa ccaaattgcg ccatatccgc gaaggtgcac cagcacaaat taccctctat
      901 agtgataaca aaacgttaca gggtcacgcg tccagtatcg gtcgggcgat ttatgatcag
      961 agcgttgaaa gtgactcaag ccttattccg gatgtgaaac ctaacgtccc ctgggttcgc
     1021 ctcgcccaac gcgttcccgt ccgctttacg ctggataaag tcccaggcga tgtcacgctg
     1081 gtgtcgggca ccacctgtag cattgccgta ggtcaataat gaacgcatcg tcatggtcct
     1141 tgcgcaattt gccctggttc agggccacgc tggcgcaatg gcgttatgcg ttacgcaata
     1201 ccattgccat gtgtctggcg ctgacggttg cctattattt gaatcttgat gagccctatt
     1261 gggcgatgac ctcggctgcg gtggttagct ttcccaccgt tggcggtgtc atcagcaaaa
     1321 gcctcggacg catcgctggc agtttgctcg gagccattgc ggcactgctt cttgccggac
     1381 atacgctcaa tgagccgtgg ttttttctat tgagcatgtc ggcgtggctt ggcttttgta
     1441 cctgggcctg tgcgcacttc acgaataacg tcgcgtatgc atttcaactg gcgggctaca
     1501 cggctgccat catcgccttt ccgatggtta atattactga ggccagccag ctgtgggata
     1561 tcgctcaggc gcgtgtttgc gaggtgatag tcggtatttt gtgcggcggc atgatgatga
     1621 tgatcctgcc gagcagttcc gatgctacag cccttttaac cgcattgaaa aacatgcacg
     1681 cccgattact ggaacatgcc agtttactct ggcggcctga aacaaccgat gccattcgtg
     1741 cagcacatga aggggtgatt gggcagatac tgaccatgaa tttgctgcgt atccaggctt
     1801 tctggagcca ctatcgtttt cgccagcaaa acgcgcgtct taatgcgcta ctccaccagc
     1861 aattacgtat gaccagtgtc atctccagcc tgcgacgtat gttgctcaac tggccctcac
     1921 cgccagatgc cacgcgagaa attctcgaac agctgctgac ggcgctcgcc agttcgcaaa
     1981 cagatgttta caccgtcgca cgtattatct ccccgctacg cccgaccaac gtcgccgact
     2041 accggcacgt cgccttctgg cagcgactac gttatttttg ccgcctttat ctgcaaagta
     2101 gtcaggaatt acatcgtctg caaagcggtg tagatgatca tgccagactc ccacggacat
     2161 ccggcctggc tcgtcatacc gataacgccg aagctatgtg gagcgggctg cgtacatttt
     2221 gtacgttgat gatgattggc gcatggagta ttgcttcgca atgggatgcc ggtgccaatg
     2281 ctttaacgct ggcagcaatt agctgcgtac tctactccgc cgtcgcggca ccgtttaagt
     2341 cgttgtcact gctgatgcgc acgctggtgt tactttcgct attcagcttt gtggtcaaat
     2401 ttggcctgat ggtgcagatt agcgatctgt ggcaattttt actgtttctc tttccactgc
     2461 tggcgacgat gcagcttctt aaattgcaga tgtccaaatt tgccgcactg tgggggcaac
     2521 tgattgtttt tatgggttcg tttatcgctg tcactaatcc cccggtgtat gattttgctg
     2581 attttcttaa cgataatctg gcaaaaattg ttggcgtcgc gttggcgtgg ttagcgttcg
     2641 ccattctgcg tccaggatcg gatgctcgta aaagccgccg ccatattcgc gcgctgcgcc
     2701 gggattttgt cgatcaacta agccgccatc caacactgag tgaaagcgaa tttgaatcgc
     2761 tcacttatca tcacgtcagt cagttgagta acagccagga tgcgctggct cgccgttggt
     2821 tattacgctg gggtgtagtg ttgctgaact gttctcatgt tgtctggcaa ttgcgcgact
     2881 gggaatcgcg ttccgatccg ttatcgcgag tacgggataa ctgtatttca ctgttgcggg
     2941 gagtgatgag tgagcgtggc gttcagcaaa aatcactggc ggccacactt gaagaattac
     3001 agcggatttg cgacagcctt gcccgtcatc atcaacctgc cgcccgagag ctggcggcaa
     3061 ttgtctggcg gctgtactgc tcgctttcgc aacttgagca agcaccgccg caaggtacgc
     3121 tggcttctta attacttaat tacaccacag gcatagcgtt caccgccacc gcccagcggt
     3181 ttgggttgat cggacatatt atcgccgcca acgtggacca tcagcgcttt gtctttgact
     3241 tcatccagtg atttcagacg aggcgcgatg acggcatcgg tcgccttgcc gtcattattg
     3301 actaccagtg caggcagatc gcctaaatgc ccggcacctt ctggcccttc atgtttaccg
     3361 gtattttgtg gatcaagatg cccgcctgcg gattctgcgg cgctggcttt gccatctttg
     3421 gtggctggct ggcagcttcc tttggcatga atatggaagc catgttcacc gggaggtaat
     3481 gctttcagat cgggcgaaaa ctccagacct ttatcggttt cagtaatggt gacgctacca
     3541 attgactgcc caaccccttg cgacgtgacg aggttcatct cgactttttc actggcagct
     3601 tgtgcgccgg tcgcaacaac cagcgccaga atggccagac taaaacgttt cataggacct
     3661 ccgttcattg tgcattcctg aataagtgta aaccagtgac acatttttga acggcggcta
     3721 ttcctaaaag tgcttacggt acgtcgtaac ctagtgccgc tttacggata cgaaaccatt
     3781 gttgacgggt cattttcagt gtttctgctt cgacagctgc ccgtacgcgc tcaattttac
     3841 ctgagccgat aattggcagc ggctgcgacg gtaaacgtag tacccaggcg taaactacct
     3901 gttcaatcga gcccgcgttt aactcctctg ccaccacagc cagttcatca cgcagcggct
     3961 ggaaataatc atcattaaac agacgaccac caccaaggca ggaccacgcc atcggacgaa
     4021 tacgcagttg ttgcagttgg tcgagcgtgc catccagcag taacggctga tgcaccgggg
     4081 atatttccac ctgattagtg gcaagagtaa atggcaggcg tgattgcaac agcgcaaatt
     4141 gagcaggtgt aaagtttgat acaccaaaat gacgcacttt gccgctctga tgcagatgtt
     4201 taaaggcgtc cgccacttca tcggcatcca ttaacgggtc tggtcggtgg attaacagca
     4261 gatccagatg atcggtcgcg agattaatta gcgactgttc ggcgctctta atgatgtgat
     4321 cgcggtcagt gatgtaatgg ccaatgacgt tttcttcaca cgcggtcgtc gcgataccgc
     4381 atttactgac gatttccatc cgttcacgca ggtgaggtgc cagtttcagc gcctcgccaa
     4441 acgccgcttc gcactgatag ccaccataaa tatcagcatg gtccacggtg gtcacgccga
     4501 gatccagatg ctcttcaata aaactgacca actggcgggc ggacatgttc cagtccatca
     4561 atcgccagta gcccatcaca aaacgggaaa actccgggcc ttgcggcgca atagtaatac
     4621 gctgaaccat aatcgcttcc tcttatcaga tatgagagga gtatacgcaa gattaggctc
     4681 aaaagagtga tggttgctcc ggttcgtctg atggcgctgg cttatttaca cgtaatttgc
     4741 gcattaatcg ctgccgacaa aggcgcaaca cttcttgttt ttcgccatcg ctcatcttat
     4801 tccagttaaa acgctcatcc cggctacgaa aacagccgcg acaaaatccg cgttcgtcag
     4861 actggcaaat tccccggcac gggctctgga cgggaaagaa ctctaattgc tccgccacct
     4921 cgccctcctc agataagatt attaccatta ttgaagctgt taatgtccaa agtagcaact
     4981 ttgcttgcac tagaccgact ggtctactac actccaacgc atgaacaaac acaccgaaca
     5041 tgatactcgc gaacatctcc tggcgacggg cgagcaactt tgcctgcaac gtggattcac
     5101 cgggatgggg ctaagcgaac tcctaaaaac cgctgaagtg ccgaaagggt ccttctatca
     5161 ctactttcgt tctaaagaag cgtttggcgt tgctatgctt gagcgccatt acgccgcata
     5221 tcaccagcga ctgactgagt tgctgcaatc cggcgaaggt aactaccgcg accgcatact
     5281 ggcttattac cagcaaacac tgaaccagtt ttgccaacat ggaaccatca gtggttgcct
     5341 gacagtaaaa ctctctgccg aagtgtgcga tctgtcagaa gatatgcgca gcgcaatgga
     5401 taaaggggct cgcggcgtga tcgccctgct ctctcaggcg ctggaaaatg gccgtgagaa
     5461 ccattgttta accttttgtg gcgaaccgct gcaacaggca caagtgcttt acgcactgtg
     5521 gcttggtgcg aatttgcagg ccaaaatttc gcgcagtttc gagccactgg aaaacgcgct
     5581 ggcccatgta aaaaacatta ttgcgacgcc tgccgtttag caggcatttt ttatcaccag
     5641 acgaccggga gcctttatgt catctgaaaa actgtattcc ccactgaaag tgggcgcgat
     5701 cacggcggca aatcgtattt ttatggcacc gctgacgcgt ctgcgcagta ttgaaccggg
     5761 tgacatccct accccgttga tggcggaata ctatcgccaa cgtgccagtg ccggtttgat
     5821 tattagcgaa gccacgcaaa tttctgccca ggcaaaagga tacgcaggtg cacctggcat
     5881 ccatagcccg gaacaaattg ccgcatggaa aaaaattacc gctggcgttc atgctgaaaa
     5941 tggtcatatg gccgtacagc tgtggcacac cggacgcatt tctcacgcca gcctgcaacc
     6001 tggcggtcag gcaccggtag cgccttcagc acttagcgcg ggaacacgta cttctctgcg
     6061 cgatgaaaat ggtcaggcga tccgtgttga aacatccata ccgcgtgcgc ttgaattggg
     6121 agagattccg ggtatcgtca atgatttccg tcaggccatt gctaacgcgc gtgaagccgg
     6181 ttttgatctg gtagagctcc actctgctca cggttatttg ctgcatcagt tcctttcccc
     6241 ttcttcaaac catcgtaccg atcagtacgg tggcagcgtg gaaaatcgcg cgcgtctggt
     6301 gctggaagtg gtcgatgccg ggattgaaga atggggtgcc gatcgcattg gcattcgcat
     6361 ttcgccaatc ggtactttcc agaacacgga taacggcccg aatgaagaag ccgatgcact
     6421 gtatctgatt gaacaactgg gtaaacgcgg cattgcttat cttcatatgt cagaaccaga
     6481 ttgggcggga ggtgaaccat atactgatgc gtttcgcgaa aaagtacgcg cccgtttcca
     6541 cggtccgatt atcggcgcgg gtgcatacac agtagaaaaa gctgaaacgc tgatcggcaa
     6601 agggttaatt gatgcggtag catttggtcg tgactggatt gcgaacccgg atctggtcgc
     6661 ccgcttgcag cgcaaagctg agcttaaccc gcagcgtgcc gaaagtattt atggtggcgg
     6721 tgctgaaggt tacactgatt acccgacgct gtaatccaac attgcgagcg gcgcaaagcc
     6781 gccgctatac taaaacaact ttttgaatct gttagccatt ttgaggatat aaagatgcgt
     6841 cttcttcata ccatgctgcg cgttggcgat ctgcaacgct ccatcgattt ttataccaac
     6901 gtgctgggca tgaaactgct gcgtaccagc gaaaacccgg aatacaaata ctcactggcg
     6961 tttgttggtt acggcccgga aaccgaagaa gcagtgattg aactgaccta caactggggc
     7021 gtggataaat acgaactcgg cactgcttat ggtcacatcg cgcttagcgt agataacgcc
     7081 gctgaagcgt gcgaaaaaat ccgtcaaaac gggggtaacg tgacccgtga agcgggtccg
     7141 gtaaaaggcg gtactacggt tatcgcattt gtggaagatc cggacggtta caaaattgaa
     7201 ttaatcgaag agaaagacgc cggtcgcggt ctgggcaact aatctcctgc cgggcgtgaa
     7261 ctcatcgcgc ccgcatcttt actgcatcga caagcaatat ttgtcataat gcgcgctgca
     7321 atttatccgt attaagagaa tcagatgtcc gataacgctc aacttaccgg tctgtgcgac
     7381 cgttttcgtg gtttttatcc tgttgtgatc gatgttgaaa cagccggatt taacgccaaa
     7441 accgatgcgc tgcttgagat tgccgccatc accctgaaaa tggatgaaca aggctggctg
     7501 atgccggaca ccacattaca tttccacgtc gaaccatttg tcggcgcaaa tttgcaacca
     7561 gaggcccttg ccttcaacgg cattgaccca aacgatcccg atcgcggcgc ggtcagcgag
     7621 tacgaggcgc tgcacgaaat ttttaaagtt gtacgtaaag gtattaaagc gagcggttgt
     7681 aaccgcgcca ttatggtggc acacaacgcc aatttcgatc acagctttat gatggccgcc
     7741 gcagaacgcg cctcactgaa acgtaacccg ttccaccctt tcgccacttt tgacactgct
     7801 gcactggccg ggctggcact cggacaaacc gtattgtcaa aggcttgcca gacagctggc
     7861 atggacttcg acagcaccca ggcgcactcc gcgctgtacg acaccgaacg cactgctgtg
     7921 ctgttttgtg aaatcgtcaa ccgctggaaa cgtctgggag gctggccact acctgccgcc
     7981 gcagaggtgt aatcgagttg atgcctgatg acatgcaatg attcaggcat ctatagtgag
     8041 gctattccac gcatcatgca tgatattcac ggggaatagc gttaatggca aataatccaa
     8101 acccttcatc gcccctgccg gacgtgtttt caccggcgac ccgcgactgg tttcttcgcg
     8161 cctttaaaca gccgaccgct gtccagtcgc aaacctggca tgtggcggcg cgaggcgaac
     8221 atgcgctggt gattgcaccg actggctccg ggaaaacgct ggcagcattt ctctacgccc
     8281 tcgatcagct cttccgcgaa ggcggcgaag atacccgcga ggcgcataag cgtaaaacct
     8341 cacgcattct ctatatttca ccgatcaaag ctctgggcac cgacgttcag cgcaacttgc
     8401 agatcccgtt acagggtatt gccgatgaac ggcggcggcg cggcgaaacg gaagtcaatc
     8461 ttcgcgtggg gatccgtact ggcgatacgc ctgcacagga acgcagcaaa ctcacccgta
     8521 atccgccgga tattctgatc accacacccg aatcactcta tctgatgctg acctcccgcg
     8581 cgcgcgaaac gctacgcggc gtcgaaacgg taattattga tgaagtccac gcggtagcag
     8641 gcagtaaacg tggtgcgcat ctggcgttaa gtctggagcg gctcgatgcg ctgctccaca
     8701 cctcagcaca gcgaattggc ctttctgcca ctgtgcgctc agcgagcgat gtggcagcat
     8761 ttcttggtgg cgatcgcccg gttacggtag tcaacccgcc cgcaatgcgc catccgcaga
     8821 tacggattgt cgtaccggtc gccaatatga atgatgtctc atcagtcgcc agcggcaccg
     8881 gcgaagacag ccatgccggc cgggaaggct ccatctggcc atatattgaa acgggtatcc
     8941 ttgatgaagt gttgcgccat cgctcgacca ttatctttac taattcgcgc gggctggcgg
     9001 aaaaactgac ggcacgatta aatgagcttt acgccgctcg cttacagcgt tccccgtcta
     9061 tcgccgttga tgcggcccat ttcgagtcga cctccggcgc aacctctaac cgtgtacaaa
     9121 gtagcgacat ttttattgcc cgctcacacc acggctccgt ctctaaagaa caacgagcaa
     9181 tcaccgaaca ggcgctgaaa tcgggtgaat tacgctgcgt ggtcgcaacc tccagtctcg
     9241 aactggggat tgatatgggc gcggtggatc tggtgattca ggtggcaacg ccgctttctg
     9301 ttgccagtgg gttacaacgc attggacgtg ccggacatca ggttggcggt gtatctaaag
     9361 ggcttttttt cccccgaacc cggcgtgact tagtcgattc cgcagtcatt gtagagtgta
     9421 tgttcgcagg caggctggaa aacctgacac caccgcataa tccactcgac gtccttgcgc
     9481 agcaaaccgt tgccgccgcg gcgatggatg cattacaggt agacgaatgg tactcctgcg
     9541 tacgccgtgc cgcaccgtgg aaagatctgc caggacgtgt ttttgacgcc acgctggata
     9601 tgctttccgg gcgctatccc tctggcgatt tttctgcttt tcgccccaaa ctggtctgga
     9661 acagggagac cgggatattg accgcccgac ctggcgctca attgttggcg gttaccagcg
     9721 gccgcaccat tccggatcgt ggcatgtata gcgtgttatt acccgaaggt gaagaacagg
     9781 ccggttcgcg gcgggtgggt gaactggatg aggagatggt atatgagtca cgggtgaacg
     9841 atattatcac tctcggcgca acctcatggc ggatccagca aatcacccgc gatcaggtga
     9901 ttgtgactcc tgctccgggt cgttctgccc ggctcccctt ctggcgtggt gaaggtaacg
     9961 gacgtccggc agaattaggc gagatgatcg gcgattttct ccatttactg gcggatggcg
    10021 agttcttttc cgggactatt cccccgtggt tggcagaaga aaatacgatc gccaatattc
    10081 aggggttgat tgacgagcag cgcaacgcga cgggtatcgt tccaggtagt cgtcatcttg
    10141 tcctcgaacg gtgccgtgat gaaattggtg actggcgtat tattttgcac tctccctatg
    10201 gaagacgggt gcatgaaccc tgggcgctgg cgattgccgg gcgaatacat gcactatggg
    10261 gcgctgacgc gtctgtggtt gccagtgatg acggcattgt tgcgcgcatt cctgacaccg
    10321 atggtaaatt gcctgatgcc gcgatttttt tgtttgaacc agaaaagttg ctgcaaattg
    10381 tccgcgaggc ggtaggcagc tcggcacttt tcgccgcccg ttttcgcgaa tgcgccgcgc
    10441 gggcattatt aatgccgggc cgcacgccgg gccatcgcac cccgctctgg caacaacgac
    10501 tgcgcgccag tcagttgctg gaaatcgctc agggatatcc agattttccg gtcattctcg
    10561 aaaccctacg cgaatgtctg caagatgttt atgatcttcc cgcgctggaa cgtttgatgc
    10621 gtcgcctgaa cggtggcgaa attcaaatat ccgatgtaac gaccactacg ccctcgcctt
    10681 tcgccgccag tttattgttc ggctatgtcg cggaatttat gtaccagagc gacgccccgc
    10741 tggcagagcg ccgggcatcc gtactgtcgc tggacagcga gttactgcgc aatctactcg
    10801 gacaggtcga tccgggggaa ttactcgacc cgcaggtcat tcgccaggtg gaagaagagt
    10861 tgcaacgact ggctcctggc agaagagcga aaggtgaaga aggattgttc gacctgctgc
    10921 gcgaactggc tccaatgacc gttgaagacc tggcgcaacg gcatacaggc agcagtgaag
    10981 atattgcgtc gtatctggaa aatcttcttg cagtaaaacg aatcttcccg acgatgatta
    11041 gcgggcagga gcgtcttgcc tgtatggatg atgccgccag gctgcgcgat gccctcggcg
    11101 tacgactacc agagtcactg ccagagattt atttgcaccg agtcagttac ccgcttcgcg
    11161 acctctttct gcgctatctc cgggctcatg ctctggtcac ggctgaacaa ctggctcatg
    11221 agtttagtct cggtattgcc attgtcgaag agcaacttca gcaactgcgc gaacagggtc
    11281 tggtgatgaa tctgcaacaa gacatctggg tgagcgatga agtatttcgt cgtctgcgtt
    11341 tgcgctcgct gcaagccgcc agagaagcga cacgtcccgt tgcggccacg acctatgcgc
    11401 gattgctgct ggaacgtcag ggcgtattac ccgccaccga tggtagcccg gcactctttg
    11461 cctcaacatc gccaggcgtt tatgagggcg tggatggcgt catgcgggtg atcgaacagc
    11521 ttgcaggagt cggtttaccc gcctcactct gggaaagcca gatcctgcct gcccgcgtac
    11581 gcgactattc gccagaaatg ctcgatgaat tactggcaac cggtgcggtt atctggtcgg
    11641 ggcaaaaaaa gctgggtgaa gatgacggtc tggtggcact gcatctacag gaatatgccg
    11701 cagaatcgtt cactcccgcc gaagcagatc aggcgaatcg ttcggcgctg cagcaagcga
    11761 taatcgctgt tctggctgac ggaggagcct ggtttgcaca acaaatcagc caacggatac
    11821 gcgataaaat cggcgaatcg gttgatccct cggctctgca agaggcgtta tgggcgctgg
    11881 tctggcaagg cgtcattacc agcgacattt gggcaccgtt acgcgccctc acccgtagca
    11941 gttccaacgc acgcacctcg actcgccgca gtcaccgggc tcgtcgtggg cgtcctgtct
    12001 atgcgcaacc cgtctcgccg ctggtatctt acaacacacc aaagctggcc ggacgctggt
    12061 cgttattgca ggtggagcca ctaaacgata ccgaaaggat gctggcgctg gcggaaaata
    12121 tgctcgaccg ctacggcatc atcagtcgtc aggcggtgat agccgaaaat atccctggcg
    12181 gttttccatc gatgcaaacg ctttgtcgaa gtatggaaga ctccgggcga attatgcggg
    12241 gtcgttttgt agaaggtctg ggtggcgcgc aattcgctga acgtctgact attgaccgat
    12301 tgcgtgacct ggcgacacaa accgcgcaaa cgcgccacta tacaccagtg gcgctctccg
    12361 ccaacgatcc ggctaatgtg tggggaaatc ttctgccctg gcctgcacat ccggcaacgc
    12421 tggttccaac gcgtcgggcg ggtgcgctgg tggtcgtttc tggcggcaaa ttgttacttt
    12481 atctggcgca aggtggcaaa aaaatgctgg tctggcagga aaaagaggaa ttactcgccc
    12541 cagaggtttt ccacgcgctg actaccgcac tgcgtcgcga accacggctg cgctttacgc
    12601 tgacagaagt gaatgatcta ccagtccgac aaacgccgat gtttacgctg ctgcgtgagg
    12661 cgggattttc aagttcgcca caagggctgg attgggaata gataaagaac tgacggatgc
    12721 ccgttcgcat ccgtcagtat tgcaggacgg attattccgc gtccggctct tcagacttgt
    12781 atttagcggc agtttctttg atcagctgct gcagttcgcc acgctgatac atttcgatca
    12841 cgatatcaca accgccgacc agctcgccgt caacccacag ttgcgggaag gtcggccagt
    12901 tagcatattt cggcagttcc gcacgaatgt ccggattctg caggatatca acataggcaa
    12961 aacgttcgcc acatgcggca agcgcctgga ctgcctgggc agagaaaccg cagctcggca
    13021 gtttcggtga acctttcatg tacagcagga tcgggttttc agcaatctgg cgttggattt
    13081 tttcgatagt ggtgctcatt gtcttgcttc ctcaaacttc tttacggcag taatctgaca
    13141 ttgtagcggg tcagtgcggc atcggaaaat aacattttca tcacgctttt gctattttat
    13201 ccctttgctc tatttgttgc atttcaaata ttcgtttctt ccctgacgca cggcaaactg
    13261 gcgtatttat aaccattttt agctttcact gctgcatttt tttgcgctcg ccgacgaaac
    13321 gtatttttta acaataaaag ctattaactt tctcttcttc tatgcattag aatcatcaag
    13381 ttttgtaaat cagacgcagg catgatagac ctgccttaac agagggacgc tcagtggcgc
    13441 ggataaaccg tatttcgatc acgctctgtg ctttactttt taccaccctg cctttaacgc
    13501 ctatggccca tgcttcaaag caagccaggg agagttctgc taccactcat atcaccaaaa
    13561 aagcagataa aaagaaaagc acggcaacca ccaaaaagac ccagaaagcc agccagaaaa
    13621 cagcgaaaaa agccgccagt aaaagtacga ccaaaagcaa aaccgcttct tccgttaaaa
    13681 aatcttccat taccgcttct aaaaacgcca aaactcgcag caaacacgcc gtcaataaaa
    13741 cggcctcagc cagctttacc gaaaagtgta ccaaacgtaa gggctataaa tcgcattgtg
    13801 tgaaagtcaa aaatgccgcg tcaggaaccc ttgccgacgc gcacaaagcg aaggtgcaaa
    13861 aagccacaaa agtggcaatg aataaactga tgcagcaaat tggtaagcca tatcgttggg
    13921 gtggcagctc accgcgtacc ggttttgatt gcagcggtct ggtttattac gcttataaag
    13981 atttggtgaa aattcgtatt ccgcgtacgg cgaatgaaat gtatcacctg cgcgatgcag
    14041 cgccaatcga acgtagtgaa ctgaaaaacg gcgacctggt cttcttccgt actcagggac
    14101 gcggtacagc cgatcatgtc ggcgtgtatg tcggcaacgg caaatttatt cagtcaccgc
    14161 gcacgggcca agaaattcaa atcacttctc tcagtgaaga ctactggcag cgccactatg
    14221 ttggcgctcg ccgggtaatg accccaaaaa cacttcgcta aaactttacc ctgttgttac
    14281 ggcaacaggg taagttcatc ttttgtctca ccttttaatt tgctacccta tccatacgca
    14341 caataaggct attgtacgta tgcaaattaa taataaagga gagtagcaat gtcattcgaa
    14401 ttacctgcac taccatatgc taaagatgct ctggcaccgc acatttctgc ggaaaccatc
    14461 gagtatcact acggcaagca tcatcagact tatgtcacta acctgaacaa cctgatcaaa
    14521 ggtaccgcgt ttgaaggtaa atcactggaa gagattattc gcagctctga aggtggcgta
    14581 ttcaacaacg cagctcaggt ctggaaccat actttctact ggaactgcct ggcaccgaac
    14641 gcgggtggcg aaccgactgg aaaagtcgct gaagctatcg ccgcatcttt tggcagcttt
    14701 gccgatttca aagctcagtt tactgatgca gcgatcaaaa actttggttc tggctggacc
    14761 tggctggtga aaaacagcga tggcaaactg gctatcgttt caacctctaa cgcgggtact
    14821 ccactgacca ccgatgcgac tccgctgctg accgttgatg tctgggaaca cgcttattac
    14881 atcgactatc gcaatgcacg tcctggctat ctggagcact tctgggcgct ggtgaactgg
    14941 gaattcgtag cgaaaaatct cgctgcataa taactgatgg caaatgcaac attacctgat
    15001 gcgcttcgct taccatgcca acgcggatga ggcgttaaca gcgcattcgg caagataaat
    15061 cgcacgttgc cagcaactgt aacgcagaag gttatccttc tgcgtttttg tttaattagc
    15121 tgttggcaac gcaaactgtt tcaggttgtt ttctggctga cataaacacc agcaataatg
    15181 ccagtcccgc gacaatcgcc cccatcaccg gcacaaaact gtatcccagc ccagcggaaa
    15241 ttaccgcacc accagcagct gctcccagcg catttccaag attaaaggct ccaatattga
    15301 ctgatgaaga cagacctggc gcttcactgg cgacacgcat cacgcgcatc tgtaacggcg
    15361 gtacgaccgc aaaggttgca gctccccaca ctaccatgct aatagctgcg ccgaactcat
    15421 tgcgggccag gaacgggatt gccagcataa tcaccatcag caacaacaaa aagcctttca
    15481 acgtgccgtt aactgaacga tctgccagtt tgccgccgag atagttaccg atagaaaatc
    15541 cgacaccaat cagcaccagc attgccgtga cgaacaccgg cgttacgtgg gtaatacttt
    15601 gcagtaccgg agagatatag gtgtagaggg taaacattgc accagcgccc agtaccgtcg
    15661 tcagcaatgc agacagcacc tgcggacgca ttaataccgc cagctctttt ttcacttcag
    15721 gtcgcgcccc tgcgccacct ttaggtaatg agaagaacag acttaccatt gaaatcacgc
    15781 ccagccccgc tgttgccaga aatgacatcc gccagccgat ggtttcgccc aaccaggtcg
    15841 ccgccggcac gccaccgata tttgccaggg ttaaccccat aaacatagtg gcaactgcgc
    15901 tagcctgttt atgttttggc actacgcttg cggccacgac tgaacccaaa ccaaaaaatg
    15961 ccccgtgatt caggctggtc aaaatgcgtg aaagcatcag ggtcatataa tccggcgcga
    16021 tggcggaaag tacgttgcca agcgtgaaaa tcgccatcag gaaaatcaac gcactgcggc
    16081 gggcacgatg agaaagtaga agcgtcatca gcggcgcgcc aaccattacg ccaactgcat
    16141 aagcactgat taacattccg gcagcgggaa tcgagacgtc cacaccgcgc gcaatgacgg
    16201 gcaacaagcc cattggcgag aactccgttg tcccgatacc aaacgcgcca atcgccagcg
    16261 ccagcaacgg atagttaatt ttcatgcctt atctccacct cttcgcgtca ttacgcgata
    16321 ttcattaaag tggcgaaagc atgacagtaa tcacaaaaaa atgaaaataa caaaaagaga
    16381 aaacactttt gccattttgc taacaaacag gaaggagatg cgagggagaa cgcgctccct
    16441 cgagaggaaa tcagtgcagc gcggcagtca aacccacggc cacgatcaaa ccgaggacga
    16501 taatcgttgt taccagtgaa aatttaaggt cggtgctcat caagttttct ccttttttat
    16561 taccacacaa aaagtgatat tacgcatttt tacacactgt gatgaaaaaa tctcccgtca
    16621 tttataatga taagtgtttt tactacttcc ccttttcgtc aagatcggcc aaaattccac
    16681 gcttacatta tttgcgtact ggccattgac cccttcctga cgctccgtgt cgtttttccg
    16741 gcgtaccgca acatttttgt tgtgcgtaag gtgtgtaaag gcaaacgttt accttgcgaa
    16801 tttgcaggag ctgaagttag ggtctggagt gaaatggaat ggcaacaata aaagatgtag
    16861 cgaaacgagc aaacgtttcc actacaactg tgtcacacgt gatcaacaaa acacgtttcg
    16921 tcgctgaaga aacgcgcaat gccgtgtggg cagcaatcaa agaattacac tactccccta
    16981 gcgcggtggc gcgtagcctg aaggttaacc acaccaagtc tatcggtttg ctggcgacca
    17041 gcagcgaagc agcctatttt gccgagatca ttgaagcggt cgaaaaaaat tgcttccaga
    17101 aaggttacac cctgattctg ggcaatgcgt ggaacaatct tgagaaacag cgggcttatc
    17161 tgtcgatgat ggcgcaaaaa cgcgtcgatg gtctgctggt gatgtgttct gagtacccag
    17221 agccgttgct ggcgatgctg gaagagtatc gccatatccc aatggtggtg atggactggg
    17281 gtgaagcaaa agctgacttc accgatgcgg tcattgataa cgcgttcgaa ggcggctaca
    17341 tggccgggcg ttatctgatc gaacgcggtc atcgcgaaat cggcgttatc cctggcccgc
    17401 tggaacgtaa caccggcgca ggccgtcttg ccggttttat gaaggcgatg gaagaagcga
    17461 tgatcaaagt gccggaaagc tggattgtgc agggtgactt tgaacctgaa tccggttatc
    17521 gcgccatgca gcaaatcctg tcgcagtcgc atcgccctac tgccgtcttc tgtggtggcg
    17581 atatcatggc aatgggcgca ctttgtgctg ctgatgaaat gggtctgcgc gtgccgcagg
    17641 atgtttcgct gatcggttat gataacgtgc gcaacgcccg ctattttacg ccggcgctga
    17701 ccacgatcca tcagccaaaa gattcgctgg gtgaaacagc gttcaacatg ctgttggatc
    17761 gtatcgtcaa caaacgtgaa gaaccgcagt ccattgaagt gcatccgcgg ttgattgaac
    17821 gccgctccgt ggctgacggc ccgttccgcg actatcgtcg ttaattaccc gttgcgggag
    17881 tctcttccgg ctcccgcagc cactccttat tcagcgtctc actatcgccg agatactcaa
    17941 gcaaccaggt taacgcaggc gacatatcat tttgctgcca tgtcagacaa catgccgaat
    18001 ccgggaaggg gttttccagt tctagtgcta cccacttccc ctcattaagc cacggtttgg
    18061 cgaaatgtgt tggcaccatc cctatgcata atcctgccga gatacaggtt gccgatgatt
    18121 cccagtcagg cacgacaagt cttttttggt tatccagcaa ccaggtaata cgtttaggta
    18181 gcgttcgcga ggtgtcttcg cgcaccaacg acggccagtt gcgcaacgta tcatcgctga
    18241 acgggccatc catcaacgcc agcgggtggt ggctggcaac aacgcaactc cagcttaaca
    18301 tccccatatc ccggaaggca taacgaccgc ctaccggaat cgcgcgtgtt gcgccaatcg
    18361 ccagttccac gcgcccgtcg gaaagtgcat cccagacacc gttgaacact tcctgaaaga
    18421 caagaagttc gacatcatca aaatggcgat aaaaatcaac gatcatctgc cgtgttcgct
    18481 ctggcctgac aatattatcc actgcgatag ctaactgacc gcgccagccg ttcgctatct
    18541 gctgacattg ctggcgggtg atctgcattt ttttgacaac agagcgtcct tctttgagaa
    18601 accacgcccc ggcagcggtc agttccacat cacggtgacg tcgttcaaaa agcggcaccg
    18661 ccagccactc ttccagctga cgcacggtat agctgaccgc agaaggaacg cgatgcaact
    18721 cctgggccgc ggcgctaaaa ctaccattac gcgctaccgc atcaacaact tccagagaat
    18781 attctgacca catagtctgc ctgcaaaatt tttgaaacca atcatcaaat attaccgttt
    18841 cacaacacta atttcactcc ctacactttg cggcggtgtt taattgagag atttagagaa
    18901 tatacatgca acctgggaaa agatttttag tctggctggc aggtttaagc gtactcggtt
    18961 ttctggcaac cgatatgtat ctgcctgctt tcgccgccat acaggccgac ctgcaaacgc
    19021 ctgcgtctgc tgtcagtgcc agccttagtc tgttccttgc cggttttgcc gcagcccagc
    19081 ttctgtgggg gccactctcc gaccgttatg gtcgtaaacc ggtgttattc atcggcctga
    19141 caatttttgc gttaggtagt ctggggatgc tgtgggtaga aaacgccgcc acgctgctga
    19201 tattgcgttt tgtacaggct gtgggtgtct gcgccgcggc ggttatctgg caagcattag
    19261 tgacagatta ttatccttca cagaaagtta accgtatttt tgcgaccatc atgccgctgg
    19321 tgggtctgtc tccggcactg gctcctctgt taggaagctg gctgctggtc catttttcct
    19381 ggcaggcgat tttcgccacc ctgtttgcca ttaccgtggt gctgattctg cctattttct
    19441 ggctcaaacc cacgacgaag gcccgtaaca atagtcagga tggtctgacc tttaccgacc
    19501 tgctacgttc taaaacctat cgcggcaacg tgctgatata cgcggcctgt tcagccagtt
    19561 tttttgcatg gctgaccggt tcaccgttca tccttagtga aatggattac agcccggcag
    19621 ttattggttt aagttatgtc ccgcaaacta tcgcgtttct gattggtggt tatggctgtc
    19681 gcgccgcgct gcagaaatgg caaggcaagc agttattacc gtggttgctg gtactgtttg
    19741 ctgtcagcgt cattgcgacc tgggctgcgg gcttcattag ccatgtgtcg ttggtcgaaa
    19801 tcctgatccc attctgtgtg atggcgattg ccaatggcgc gatctacccg attgttgtcg
    19861 cccaggcgct gcgtcccttc ccacatgcaa ctggtcgcgc cgcagcgttg cagaacactc
    19921 tacaactggg tctgtgcttc ctcgcaagtc tggtagtttc ctggcttatc agtatcagca
    19981 cgccattgct caccaccacc agcgtgatgt tatcaacagt agtgctggtc gctctgggtt
    20041 acatgatgca acgttgtgaa gaagttggct gccagaatca tggcaatgcc gaagtcgctc
    20101 atagcgaatc acactgatct atatcgatat acttatactt aggctgctaa caaaattttg
    20161 ttgtatgatt gaaattagcg gcctatacta atttcgagtt gttaaaacta cgataaatat
    20221 tatgttatta cggggacagg atcgttcccg actcactatg gatggtcatt tcggcaaggg
    20281 ttcctccttt ccctctgttc tacgtcggat tatagactcg cggttttttc tgcgagattt
    20341 ctcacaaagc ccaaaaagcg tctacgctgt tttaaggttc tgatcaccga ccagtgatgg
    20401 agaaactatg agttcatcgt gtatagaaga agtcagtgta ccggatgaca actggtaccg
    20461 tatcgccaac gaattactta gccgtgccgg tatagccatt aacggttctg ccccggcgga
    20521 tattcgtgtc aaaaaccccg atttttttaa acgcgttctg caggaaggtt ctttggggtt
    20581 aggcgaaagt tatatggatg gctggtggga atgtgaccga ctggatatgt tttttagcaa
    20641 agtcttacgc gcaggtctcg agaaccaact tccccatcat ttcaaagaca cgctacgtat
    20701 tgccggcgct cgtctcttca atctgcagag taaaaaacgt gcctggatag tcggcaaaga
    20761 gcattacgat ttgggtaatg acttgttcag ccgcatgctt gatcccttca tgcaatattc
    20821 ctgtgcttac tggaaagatg ccgataatct ggaatcagcc cagcaggcga agctcaaaat
    20881 gatttgtgaa aaattgcagt taaaaccagg gatgcgcgta ctggatattg gctgtggctg
    20941 gggtggactg gcgcactaca tggcgtctaa ttatgacgta agcgtggtcg gcgtcaccat
    21001 ttctgccgaa cagcaaaaaa tggctcagga acgctgtgaa ggcctggatg tcaccatttt
    21061 gctgcaagat tatcgtgacc tgaacgacca gtttgatcgt attgtttctg tggggatgtt
    21121 cgaacacgtc ggaccgaaaa attacgatac ctattttgcg gtggtggatc gtaatttgaa
    21181 accggaaggt atattcctgc tccataccat cggttcgaaa aaaaccgatc tgaatgttga
    21241 tccctggatt aataaatata tttttccgaa cggttgcctg ccctctgtac gccagattgc
    21301 tcagtccagc gaaccccact ttgtgatgga agactggcat aacttcggtg ctgattacga
    21361 tactacattg atggcgtggt atgaacgatt cctcgccgca tggccagaaa ttgcggataa
    21421 ctacagtgaa cgctttaaac ggatgtttac ctattatctg aatgcctgtg caggtgcttt
    21481 ccgcgcccgt gatattcagc tctggcaggt cgtgttctca cgcggtgttg aaaacggcct
    21541 tcgagtcgct cgctaaaggc tattctatcg ccccctctcc gggggcgatt tcagatcagg
    21601 cttctgtacc tggttgattc atggcatttt ctcgtgccgc cagcacacgt tctaccgtat
    21661 ctaccactgc ctgagtttgt ggatcgattt caatgttgac gcgtgcgccg agttttttct
    21721 tcccaagagt cgtgcgttcg agtgtttccg gaattaaatg gacgcaaaaa cgcgttggcg
    21781 tgacttcgcc gacggtcagg ctaataccgt cgatgccaat aaatcctttg tacaggatat
    21841 atttcatcaa ctgactatcc tggactttaa accagatctg acgattattt tccgaggtta
    21901 atattttcgc tacttcagcg gtggtcataa tatgacctga cattaagtgt ccgccaattt
    21961 catcgctgaa tttcgccgca cgctcaacgt ttacccaatc ccccactttt aaatcgccaa
    22021 gattagtaat gcgtaacgtt tctttcatca ggtcaaaact gacatggttg ccgttaattt
    22081 ccgtcacggt caggcagcaa ccgttatgcg ccacggaagc accggtttcc agaccgtcca
    22141 gcatgtggtc gggtaactcc accacatgcg tgcgaaaatt tggtttctcg tcaatcgaca
    22201 ccagttttgc ggtgccctgt acaatccccg taaacatact tacaactcct gaaatcagtt
    22261 aagacattct gttcagcaca atagcaggtg gaaaacgccc ttaccagtga aggggtaaga
    22321 atggctattt tttcactgga gaattaataa atcctcgcta caatagactg aatttcccct
    22381 gcttcttctt tttgctgccc attcaggcgg ctttttagtc tctcatataa ctacaaataa
    22441 aaggtgttca cgtgcagaag tatatcagtg aagcgcgtct gttattagca ttagcaatcc
    22501 cggtgattct cgcgcaaatc gcccaaactg cgatgggttt tgtcgatacc gtgatggcgg
    22561 gcggctatag tgccaccgac atggcggcgg tcgctatcgg tacttctatc tggcttccgg
    22621 cgatcctctt tggtcacgga ctgctgctgg cattaacgcc ggttatcgcg caattaaatg
    22681 gttccggtcg acgtgagcgc attgcgcatc aggtgcgaca aggtttctgg ctggcgggtt
    22741 ttgtttccgt tctcattatg ctggtgctgt ggaatgcagg ctacattatc cgctccatgg
    22801 aaaacatcga tccggctctg gcggacaaag ccgtgggtta tctgcgtgcg ttgctatggg
    22861 gcgcgccggg ctatcttttc ttccaggttg cccgtaacca gtgtgaaggt ctggcaaaaa
    22921 ccaagccggg tatggtgatg ggctttatcg gcctgctggt gaacattccg gttaactata
    22981 tctttattta tggtcatttc ggtatgcctg agctcggtgg agttggttgt ggcgtggcta
    23041 ctgcggcggt gtattgggtc atgttccttg ccatggtttc ttacattaaa cgcgcccgct
    23101 cgatgcgcga tattcgtaac gaaaaaggca ccgcgaagcc cgatcctgcg gttatgaaac
    23161 gactgattca actcggtttg ccaattgcgc tggcactgtt ctttgaagta acgctgtttg
    23221 ccgtcgtggc tctgttagtg tctccgctcg gtattgttga tgtcgcagga catcagattg
    23281 ccctgaactt tagttcactg atgttcgtgc ttcccatgtc gctggcggca gcggtaacca
    23341 tccgcgtagg ttatcgtctg ggtcaaggct caacgctgga tgcgcaaacc gctgcgcgga
    23401 ccgggcttat ggtgggtgtc tgtatggcaa ccctgacggc catattcacg gtttcactgc
    23461 gggagcaaat cgccctgttg tacaacgaca atcccgaggt tgtaacgctg gctgcacatt
    23521 tgatgttgct ggcggcggta tatcagattt ctgactctat ccaggtgatt ggcagtggaa
    23581 ttttgcgtgg ttataaagat acgcgttcca ttttctatat cacctttacg gcttactggg
    23641 tgctgggctt gccaagcggc tatattctgg cactgaccga tctggtcgtt gaacctatgg
    23701 ggccagcagg cttctggata ggctttatta ttggcctgac gtcggcagcc attatgatga
    23761 tgctgcgtat gcggttcctg caacgtctgc cgtcagccat cattctgcaa cgagcatccc
    23821 gctaataaag acaaggcgca accttcacgg gttgcgcctg tgtttttacg caggctggag
    23881 cgttgcgccg atgccatctt ggtctggctg taatttcaga gagttacaca gagtttgccg
    23941 actggcatct gttatctcaa cgtgtatatc accggtaact tcccctttca cattgatgaa
    24001 gtcaggtgaa atttctttcg cactggtttg caggcaaatg gctccctctc cacgcaggcg
    24061 tttcactttc agttgcacct gcgttgccgc atcagtgatc agccacagtt ttccgccatt
    24121 cagattgatg ttaccaatac caaaagtgac actgtcacct tttaccgtac tgccgtcgag
    24181 aatcaacaca ccgccctgct gtacagtaat cgttgattgt tgcagctctg cgccatagcc
    24241 aataatcatt gaagcgccgt ccagtaatgc aacactcccg gtcagttcaa ccggtgcacg
    24301 atcttcctct acgacaaaat tttcgtccgc gctggtgggt gacaacaaaa tcactgtccc
    24361 accttgtatt accgcgtttt ccagcgtacc agcatgcact tcagcccgac cacccacatt
    24421 aacggacagg ttttgcgtct taccggaact gtagagctga ataccatccg tccccagacg
    24481 ataaatggat ccattctcaa caatagtgtc agagtcttca ccaccgagat tattcatggt
    24541 gccaccagca agcaacgtat cactggcttt ccccgccagc ataaaaacgc cgccctgatt
    24601 aatctcacaa ttgcaggctt ccccacctgg atacaccaac aattcaccac cttcatctac
    24661 cacgaccgct gaggttgtcc cattgaccaa caggccgccc tcttgatcga gaatgatttt
    24721 ttccgcgcga tggccttcca gtacacgcag gttaccgccg ttttccagca acaaaccgca
    24781 ggcataacct ttatcaacgc tgaattcacc ttcgggatgg cggccattaa ccgtagcgag
    24841 agtagagaga ctaattgcgc ctccctcgga ctgaacaata tctgtggcaa tggcaccgtc
    24901 gctgaccgat agtgtgccat tgttaccgta ctgtgtgcat ttggctatcc caccggttgc
    24961 cactttcaac cagccagccc ccgtgaccac ggtattttcg gctcttccgc cagcttcaac
    25021 aatcaactcg ccttcagtaa gttgcgtcgc gccaataacc tcgccggtct taacaatttg
    25081 cacgttcccg tcgctcatca agtttttcgc atcagatccc atgatttatt cctttgctgc
    25141 atctgtgtgc ctttattgct acctaagtgt aaaggctacg aaggatttat ccacgacaga
    25201 tttgagatgg tggcaaacaa ctctgtttaa actctgatac acggattatt gggttgtgtc
    25261 agatgtaaat gcgatcctga ataaaaatca cccttgcaaa tcaacaaaat atagccaatt
    25321 tgcttaaaag ctctgcaaac ggtcgaaata gcgcagaaaa ttacgttttg cctcttgcca
    25381 ccttcccact ctgccgctaa tattcgtccc cgttgtcacc tacaacgttg cgttcatagc
    25441 tcagttggtt agagcaccac cttgacatgg tgggggtcgt tggttcgagt ccaattgaac
    25501 gcaccatcct gcgtccgtag ctcagttggt tagagcacca ccttgacatg gtgggggtcg
    25561 gtggttcgag tccactcgga cgcaccagat tttcttaatc tggtcttctt ctttgtccct
    25621 ctgtttcttc tctgtatcca atacgttaaa agatctacac tgtcttcgtg tgcgttatca
    25681 gaaggagaat cgctgtggca actttgttac aacttcattt tgcttttaat ggcccatttg
    25741 gcgacgcaat ggctgagcag cttaaaccgc ttgctgagtc gattaatcag gaacctggtt
    25801 ttctgtggaa ggtttggaca gaaagtgaaa agaaccacga agccggtggg atctaccttt
    25861 tctctgatga aaaaagcgcc cttacctatc tggaaaaaca tactgcccga ctgaaaaatc
    25921 ttggcgttga ggaagttgtc gccaaagttt tcgatgtcaa tgtgccactg agtcaaatca
    25981 atcaggcaaa acttgcctga cagattttaa tcaagggcgg ttagtgccct tttcatccct
    26041 gtctgaaatt tctcaaattc taaaaatctc aaccaaactt atctgataac actaaattcg
    26101 aaagaatgcg tacaggtaag taacaatgaa aacaattgct attgtgggtg ccgggcctac
    26161 agggatctac accttattct cgcttctaca gcaacaaacg ccactttcta tttctatctt
    26221 cgagcaggct gacgaagccg gtgtcgggat gccatacagt gatgatgaaa actcaaaact
    26281 gatgctggca aacattgcca gtattgaaat accgccgatt tattgcacgt atctcgaatg
    26341 gctacaaaag caagaagcca gccatctcca gcgttatggc gttaaaaaag aaaccttgca
    26401 cgatcgtcag tttttaccgc gaattctgct gggcgaatat ttccgcgatc aatttttacg
    26461 attagtagac caggcacgac agcaaaaatt tgcagtggct gtttatgaat catgccaggt
    26521 taccgatctg caaattacaa atgctggcgt catgctcgct acaaatctgg atttacccag
    26581 cgagacgttt gatttagtgg tgatcgcaac ggggcacgtt tggcctgatg aagaagaagc
    26641 aaccagaacg tattttccca gcccgtggtc aggcctgatg gaagcaaagg tcgatgcgtg
    26701 taacgtgggt attatgggaa catccttgag cggactggat gcggcaatgg cagtggctat
    26761 tcagcatggt tcgttcattg aagatgataa acaacacgtc acttttcacc gcgataacgc
    26821 aagtgaaaag ctaaatatta cgttaatgtc gcgcacaggt attttacccg aagccgattt
    26881 ctattgccct attccctacg agcccttaca catcatcacc gatcaggcat taaatgctga
    26941 gattcaaaaa ggcgaagtgg gccttttgga tcgggtattt agattgatag tagaggaaat
    27001 caagtttgct gatccagact ggagccaacg catagcctta gagagcctga atgtcgattc
    27061 ctttgctcaa gcctggtttg ccgagcgcaa acaacgcgac ccatttgact gggcagaaga
    27121 aaatctccag gaagtcgaac gcaataaacg agaaaaacac actgttccct ggcgttatgt
    27181 cattctgcgc ctgcatgaag ccgtacagga aattgttcca catctgaatg aacacgacca
    27241 taaacggttc agtaaaggcc ttgcccgggt tttcatcgat aattatgcgg caatcccttc
    27301 agagtctatt cgtcgactat tggccttacg tgaagcggga atcattcata ttctcgctct
    27361 cggtgaagac tacgaaatgg aaattaacga gtcgcgcacc gtcctgaaaa cggaagacaa
    27421 cagttactcg tttgacgttt ttattgatgc ccgcgggcag cgtccgctta aagtgaaaga
    27481 tattcctttc cctggactac gcgaacaatt acagaaaaca ggggatgaaa tccctgatgt
    27541 tggcgaagat tatacgttac agcaacccga agatattcgt gggcgcgtag cgttcggcgc
    27601 gttgccctgg ttgatgcacg accagccttt cgttcaggga cttacggcat gtgcagaaat
    27661 tggagaggcg atggctcggg cggtcgtaaa acctgcatcc tgtgcgcgtc ggcgtctttc
    27721 gtttgattaa aggtaaagct atcagcactc aacaaactga tgttgttcat taagacgata
    27781 gcggactccg gctcgaatat tgttttcacc ttcaatggcg acggcgaagc gaactctttc
    27841 gccgtcatga tatgcgagtg cggcgcatcc ccctggcccg agaataattg agtcgaccac
    27901 gcccgtgctc actactgttg aattttcacc gctggcaatg atgtgtacat tgtcgccgca
    27961 gttggcaatt ctggcgtttg cgccaaagct ggcgatttgc accaggtcac cattactggc
    28021 gacatgacac cgttcaccca atgtacaaac gcgtactcgc attccggtat tcgcaatccg
    28081 actactatct ccagcgctgc ttatccgcga agaattaccg gttacagcaa cccgtgcgcg
    28141 ttcaccggag ctgccaatgt gactgttaaa gccgacactt ccaatccgca cgctataacc
    28201 catactggca atttgcgcgg catacccagc agttgcgatt ctggcattat cctcacaaca
    28261 gccgatgcga gcatgatcgt cgctgcgggc aaactgactg caaaatgctc tctctccggg
    28321 atctgtggcc agtttttgca tagaagaaac ttcctgatgc gcgaagtttt catccgccag
    28381 ccattttttc cacgcatact caaccagact ctccgcccag tccgtatatc cttcctcaat
    28441 cagtgcatga tgaatatcgg cataactacc gccgcgtggg aaatggcgca gaaaccagcg
    28501 atacatcacc gctccgatac gccattctcg caggtctgct cgtgtaatga tcattcgtta
    28561 gtgccggtga tagccatcga ccatgctttt aaaggtttcg tgtggcgtac gccccgtggt
    28621 gcaaagataa agatgaccga agataaaaaa gaggcttata aatgccagag caaaatgcgc
    28681 ctgcaataac cagtatctta cgccaggaaa cacatctccc acggcttgcg gatagagaca
    28741 cagcagcccc gtcaatagta acaacggcag caatccatac atgacaccaa cataggcaac
    28801 ctgctgtaag ggattaaatt tagactgggt tgttgccggg aaaggatgtt cttccacctg
    28861 cataatgcca aacaaataga atcgcgtttg ttttgccgct cgctccagcc acccctgacg
    28921 acgaatgcga tagtggtgac cattaccccc aacggcattg atcagaacaa agccgagcca
    28981 gcatgccagt aacaaaaatc cgcaaacttc atgcaccgca accagacttt taaccgcagt
    29041 tgcgcccacc agcgcaaagt gatttatcag cccacttgcc agcaacaata caaagagtaa
    29101 cgcattcgac cagtgccata atctgaccgc tttgctgtaa agataaatcc tctcaccatg
    29161 atcggtggct gatttcttca cgccacgagc acgaagcaag gcatgtagcc ccagcaccaa
    29221 ccacatccca accagcaata gtccggcaat gatcaaccac accggccaaa attggggggt
    29281 gaactgtggc acatagttcg ccaactggct ctgaaactgt tcagcatgtt gcgacgggtt
    29341 catacatttt cctttttaat caaatgtcga ccgaaccggc gatacagatg cggtttcccg
    29401 gcgccaggta gctgatattg atagtattta ttttcctgca accacgcctg aatttctggg
    29461 ctatcttcgt gaccaaagat caatgcgtgt tccgggcagg cactgacgca aatgggcgga
    29521 aagccttttg ccagtcgggt ctcagcgcag aaatcacatt tatctgccac tttagtgaca
    29581 gggttaaggt agcgaacctg gtacgggcaa gcgccaatgc aataactgca gccgatgcac
    29641 tgcgattttt ctacccgcac gatcccctgc tcatcacgcc acgacgcccc tgtcgggcaa
    29701 acgtcgatgc aaggagcatc ttcgcagtgc tgacatgact ggcgaaaaaa gtgatactgc
    29761 gttccattgt cgttatcgat gactggaatg tgtgctatcg ataaacggct tccctgagcg
    29821 gggacgtggt tagttttacg gcatgcgcgg gcgcagatat tacagccatt gcaccgtgac
    29881 tcgtcatgaa tcatggcata ccgaacttcc ttttcttgcc tcgtattcgc taacagagaa
    29941 gacgcagaac cagtaaaaaa gattactgtt cccatcccca gaacaaattt gcgtcgagtg
    30001 aaggacatct gattatcccc taatgcagtg gctgcaactg tccaccctat ccatgacatc
    30061 cccctgaaat ttcaggggtt ctgaaactgc tctacctcga accattcgca taagccatac
    30121 gccttgccat aggtcataaa tcgctcgcta aacaagtaca acactgccgt cgatgactcc
    30181 acctgccgta tatcggcata ttcaggtgca acatccatcg tggcgatcgc ggcttcaatc
    30241 tgcgcttcct ggaagtaata tggcgcttgc atcagcatgg cgactttgta aggacgagga
    30301 tacgtctggc actcaaaacg aacggcgtgt gcaatggcgc gacaaatgtc ctgctccaca
    30361 acttgcaaag acatcgctgc gtagttttca ctcatggcct gcgttgagta atagtaatca
    30421 tcctggcttc ccgtcataca agcgatatcc gcgtagtctc cgttctgctt aatgttttcc
    30481 agcaaaactg acagttcctc ttctgcaacc gaatagggtg gcgagagaaa cacggcgcgc
    30541 gccaccagct gcccagccgc agaatgctgg cgaatgaact ctgccaggat ttcagccggt
    30601 tctggttcgg ttaccgtatc aacggggtga cgcatcccct gcaaccattg ccgcttcgcc
    30661 tcatcaattt cgctgacttc cgctaacggc aattcatcct gatggttcat ctctcccatt
    30721 ccttacgcag gcagtagatt gtgtgctgcc agatcggcgg caatatcttc cagccccaga
    30781 cgctgcaatg tttcgcgggt cggacaacca agctgtgggt cccagcccat ttctttgtag
    30841 aacatcgtga gcgaagcatg catatcgtca cgatccattt tgtctgtacc ttcagtaaag
    30901 accggaatct gcggatcctt gtcgaatacc caggaacaga taagatcgtg ttcattacgc
    30961 atatctttgg tttgcatcag ttttaccgtg taggcgcgat gcaacgtaaa gatacgctct
    31021 gcagctaaat ctaatttttc ctgagtcata tcttcgccag tgatcgcttt gaagaatttg
    31081 gcttcaagcg ccagatcgcc ccgataatta cggcttttca gcggcgaaac ggtcattggc
    31141 cagacccagt tgcacagggt gacggcgtta tgcaaacaga cccgcaacag cgaccactta
    31201 gcatatttaa tttttgcgtc attgattggc gtgtagtttt tggtttcatc gtaagcatct
    31261 tcagaaccaa aaagctcttt cgccacttca cgctgcaatt tcaatggcaa gccggaacca
    31321 ataaaattga tatgggtatg cgtcatgcag tcacggttga acatacagtt aacaatggag
    31381 ccgacctgcg ctgacgcttc attggcgtgg tgaactggat agccaaacgg cgaccagagt
    31441 ttatttttcg cgtagcccca gtactcttca cccaaattcc agcgttctgc gatggcatat
    31501 gaaccatcag ccaggtgact cagctcaccc acgcgatgcg ccagacggta gtaaaaatct
    31561 ttaatgaagt taacgtcacc cgcttccagt tgatcccagc gaatttccgc atactcttca
    31621 gcaggcagaa cacgcttgaa cacccctttg ctgtaacaat aggtaaagtc gcgatgaagc
    31681 tgaccatagt tacaccatag gccgtagtca tcgaacagat tcagaccaac caggttacca
    31741 atcacacgac catcatcttt atcttcaaaa tctttcggcc cgttcgggaa gatggtggta
    31801 tggacaaagt ttgctacaca agtgttacca cctgtgctgg gcacgccaaa ctctttcacc
    31861 cgaggaatat tcatttgggt catacaacgg atcgggcaag agtggcagcc actcattttt
    31921 actgtgtatt tctctgccgc cggacctaag tcaaaaacgg atttataggt acgaaagccg
    31981 accgtgttct gattgcctgg aggaatttcc cccgtttcaa tcggaccacc ttcagccgcg
    32041 ccccaaaaca gccctttacg tgctgtccaa cgtgacttgg gatctgaata ctctgcccac
    32101 gattgcggcg tacttggcac gacatggttg ttattcgcac cgataagttc agtcatcatg
    32161 taatcgttga gacgcttcat ctcctgacga tcggcaatgt tgaccccttt cgtcccttca
    32221 accgcaatcg ctttcaggtt tttcgaaccc attattgcgc cagttcccgc accgccgctg
    32281 tggttacggc tattcaacat gccagagaga ggaacaaggt tttccccagc ctgaccaata
    32341 gccgccacac aggtttccgg actggtcaag cgacaaattt cttccgtcgt tgcgcgcgtc
    32401 ccttttcccc ataagaaatc ggctttttca aggctaactt tgtcatcttt aatcttcagc
    32461 cataccggtg atttcgcttt cccttcgata ataatgacgt cgtatccagc gaatttcatt
    32521 tgcgctgcaa aaaagccccc catatgggca tcgacgacta aatttccttt ggtaaaagta
    32581 gaaagtgaag tgatatttac gcgaaaacta cagggggcac cagatccagt taatgggccg
    32641 gtagcaaaaa ctaatttatt cgcttcatcg aaaggtttcg tgcctggcgg tacttcgtca
    32701 tacataattt tgtagccgaa gcccatgcca ccgacaaaac ttttaaactt actggaatct
    32761 tcgagggtaa tatttcctgt cgtgagattg actcttaata tattacctgt ccaaccgtta
    32821 gccatgattt tttcctttgc aagattcaca cagtaatatc tttccactcg ataattttta
    32881 acgcccctgt tgggcaggcg tttgcgcatt caccgcataa cacacatttc gaggattttt
    32941 tactttcggt atttacggtg gccatcatcc acgggcatgc cgtcgtacag gcgctacagc
    33001 caatacaacg tttatgatcg acggtaatac agccttcttt ctgctgccag gtaatcgcac
    33061 caatcgggca gacgttcatg cattgcggct ctttgcattg acggcaggta tccgcggtat
    33121 agttgagatc gccatacagg ccaccgccag agccaacccc gttgtcgcca aagaaataat
    33181 tgcgatggat tttgatacgg gagaagaatg tccctactga gccatcgttg aagttggtac
    33241 aagagatttc acagcggtgg caacccgtgc atcgcgcacg ttgggtcacc agcacccctt
    33301 tgggtgtatt tatcaaccct actgtgccgc tatcaatatc ttcctgtttg caacccaaaa
    33361 gtgacagtaa agcaggggca attgttaaac cagcaaggcc ttttcctgat atacgcaaga
    33421 attctaatcg cgttaagcca atatttaata gtggacgatc aaccgggttc atttatttcg
    33481 catcctcttc gcaaacacag tgctgacaat tcagcagcct gatattaggc actcaactaa
    33541 tacgctgctg acgctgtcct caacaccaga tattttccct aacccgatag tgtatgagaa
    33601 aagatttaac gctgataaac taatccttta tggttaggag aaaaaataa
//
LOCUS       DADSYM010000037        33581 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2415
            SAMN08039579-rid11723173.denovo.037, whole genome shotgun sequence.
ACCESSION   DADSYM010000037 DADSYM010000000
VERSION     DADSYM010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039579
            Sequence Read Archive: SRR6362533
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 33581)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33581)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980162.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 03:39:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,484
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 4,347
            CDSs (with protein)               :: 4,347
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 43 of 85
            Pseudo Genes (incomplete)         :: 37 of 85
            Pseudo Genes (internal stop)      :: 19 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33581
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039579-rid11723173.denovo.037"
                     /strain="LGE2415"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8302 N 95.9628 W"
                     /collection_date="2015-08-24"
     gene            24..1067
                     /locus_tag="J1Y83_001470"
     CDS             24..1067
                     /locus_tag="J1Y83_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001520108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HBA7629627.1"
                     /translation="MQGIKIYTILASSLISGSIFAQTEISTANSSTVNASHVEPSAEK
                     IISPSDKLNNLFERNMSQPYILQKIGERTYYVQRYFYSTTFYVGDKGVLLFDAPEGRG
                     KYLLQAIRDVTPLPVTALVYSHYHVDHIGDSPFWNDEAKKEGVNLRIIASKATAEKMQ
                     FMNSRLPVATQVLSKKDDQFKFEKQTIELHRFVKAGHTDDHSVWLLKQEKVAHSPDLL
                     NPDQLPMMGFAVSDTLVYHDSNLRQVEMLDWKYFIGGHGNIGLHDDFKFQRQFLNDLR
                     DTTIKVRKEESFGKFMNKTANNHADFARAQREAIIKKVTEVLRPKYGHMYGYDASMPA
                     NIEMAIRLVGSYY"
     gene            complement(1210..2574)
                     /locus_tag="J1Y83_001471"
     CDS             complement(1210..2574)
                     /locus_tag="J1Y83_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntP family transporter"
                     /protein_id="HBA7629628.1"
                     /translation="MSTITLLCIALAGVIMLLLLVIKAKVQPFVALLLVSLLVALAAG
                     IPAGEVGKVMIAGMGGVLGSVTIIIGLGAMLGRMIEHSGGAESLANYFSRKLGDKRTI
                     AALTLAAFFLGIPVFFDVGFIILAPIIYGFAKVAKISPLKFGLPVAGIMLTVHVAVPP
                     HPGPVAAAGLLHADIGWLTIIGIAISIPVGIVGYFAAKIINKRQYAMSVEVLEQMQLA
                     PASEEGATKLSDKINPPGVALVTSLIVIPIAIIMAGTVSATLMPPSHPLLGTLQLIGS
                     PMVALMIALVLAFWLLALRRGWSLQHTSDIMGSALPTAAVVILVTGAGGVFGKVLVES
                     GIGKALANMLQMIDLPLLPAAFIISLALRASQGSATVAILTTGGLLSEAVMGLNPIQC
                     VLVTLAACFGGLGASHINDSGFWIVTKYLGLSVADGLKTWTVLTTILGFTGFLITWCV
                     WLVI"
     gene            complement(2663..3439)
                     /locus_tag="J1Y83_001472"
     CDS             complement(2663..3439)
                     /locus_tag="J1Y83_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HBA7629629.1"
                     /translation="MPRFAANLSMMFTEVPFIERFAAARKAGFDAVEFLFPYDYSTQQ
                     IQQQLVQNHLTLALFNTAPGDINAGEWGLSALPGREHEARADIDLALEYALALNCEQV
                     HVMAGVVPAGEDAERYRAVFIDNLRYAADRFAPHGKRILVEALSPGVKPHYLFSSQYQ
                     ALAIVEEVARDNVFIQLDTFHAQKVDGNLTHLIRDYAGKYAHVQIAGLPDRHEPDDGE
                     INYPWLFRLFDEVGYQGWIGCEYKPRGLTEEGLGWFDAWR"
     gene            complement(3444..4082)
                     /locus_tag="J1Y83_001473"
     CDS             complement(3444..4082)
                     /locus_tag="J1Y83_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase"
                     /protein_id="HBA7629630.1"
                     /translation="MSDFAKVEQSLREEMTRIASSFFQRGYATGSAGNLSLLLPDGNL
                     LATPTGSCLGNLDPQRLSKVTADGEWLSGDKPSKEVLFHLALYRNNPRCKAVVHLHST
                     WSTALSCLQGLDSSNVIRPFTPYVVMRMGNIPLVPYYRPGDKRIAQDLAELAADNQAF
                     LLANHGPVVCGESLQEAANNMEELEETAKLIFILGDRPIRYLTAGEIAELRS"
     gene            complement(4079..5341)
                     /locus_tag="J1Y83_001474"
     CDS             complement(4079..5341)
                     /locus_tag="J1Y83_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001649192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four-carbon acid sugar kinase family protein"
                     /protein_id="HBA7629631.1"
                     /translation="MIKIGVIADDFTGATDIASFLVENGLPTVQINGVPTGKMPEAID
                     ALVISLKTRSCPVVEATQQSLAALSWLQQQGCKQIYFKYCSTFDSTAKGNIGPVTDAL
                     MDALNTPFTVFSPALPVNGRTVYQGYLFVMNQLLAESGMRHHPVNPMTDSYLPRLVEA
                     QSTGRCGVVSAHVFEQGVKAVRQELARLQQEGYRYAVLDALTEHHLEIQGEALRDAPL
                     VTGGSGLAIGLARQWAQENGNQAREAGRPLAGRGVVLSGSCSQMTNRQVAHYRQIAPA
                     REVDVARCLSTETLAAYAHELAEWVLGQESVLAPLVFATASTDALAAIQQQYGAQKAS
                     QAVETLFSQLAARLAAEGVTRFIVAGGETSGVVTQSLGIKGFHIGPTISPGVPWVNAL
                     DKPVSLALKSGNFGDEAFFSRAQREFLS"
     gene            complement(5338..6246)
                     /locus_tag="J1Y83_001475"
     CDS             complement(5338..6246)
                     /locus_tag="J1Y83_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HBA7629632.1"
                     /translation="MKTGSEFHVGIVGLGSMGMGAALSCVRADLSTWGADLNSNACAT
                     LKEAGACGVSDNAATFAEKLDALLVLVVNATQVKQVLFGEKGVAQHLKPGTAVMVSST
                     IASADAQEIATALAGFGLEMLDAPVSGGAVKAANGEMTVMASGSDIAFERLAPVLEAV
                     AGKVYRIGAEPGLGSTVKIIHQLLAGVHIAAGAEAMALAARAGIPLDVMYDVVTNAAG
                     NSWMFENRMRHVVDGDYTPHSAVDIFVKDLGLVADTAKALHFPLPLASTALNMFTSAS
                     NAGYGKEDDSAVIKIFSGITLPGAKS"
     gene            6412..7209
                     /locus_tag="J1Y83_001476"
     CDS             6412..7209
                     /locus_tag="J1Y83_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HBA7629633.1"
                     /translation="MSAGQRKEQKLIPVERRQIILEMVAEKGIVSIAELTDRMNVSHM
                     TIRRDLQKLEQQGAVVLVSGGVQSPGRVAHEPSHQVKTALAMTQKAAIGKLAASLVQP
                     GRCIYLDAGTTTLAIAQHLIHMEPLTVVTNDFVIADYLLDNSNCTIIHTGGAVCRENR
                     SCVGEAAATMLRSLMIDQAFISASSWSVRGISTPAEDKVTVKRAIASASRQRVLVCDA
                     TKYGQVATWLALPLSEFDQIITDDGLPESASRALAKLDLSLLVAKNE"
     gene            complement(7260..7916)
                     /gene="pphB"
                     /locus_tag="J1Y83_001477"
     CDS             complement(7260..7916)
                     /gene="pphB"
                     /locus_tag="J1Y83_001477"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-serine/threonine phosphatase"
                     /protein_id="HBA7629634.1"
                     /translation="MPSTRYQKINAHHYRHIWAVGDIHGDYQLLQSRLHQLSFCPETD
                     LLISVGDNIDRGPESLNVLRLLNQPWFISVKGNHEAMALDAFETGDGNMWLASGGDWF
                     FDLNDSEQQEAIDLLLKFHHLPHIIEITNDTIKYVIAHADYPGKEYQFGKEIAESELL
                     WPVDRVQKSLNSELQKINGADFFIFGHMMFDNIQTFANQIYIDTGSPKSGRLSFYKIK
                     "
     gene            complement(8022..10583)
                     /gene="mutS"
                     /locus_tag="J1Y83_001478"
     CDS             complement(8022..10583)
                     /gene="mutS"
                     /locus_tag="J1Y83_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HBA7629635.1"
                     /translation="MSTIENFDAHTPMMQQYLKLKAQHPEILLFYRMGDFYELFYDDA
                     KRASQLLDISLTKRGASAGEPIPMAGIPYHAVENYLAKLVNQGESVAICEQIGDPATS
                     KGPVERKVVRIVTPGTISDEALLQERQDNLLAAIWQDSKGFGYATLDISSGRFRLSEP
                     ADRETMAAELQRTNPAELLYAEDFAEMSLIEGRRGLRRRPLWEFEIDTARQQLNLQFG
                     TRDLVGFGVENAPRGLCAAGCLLQYAKDTQRTTLPHIRSITMERQQDSIIMDAATRRN
                     LEITQNLAGGAENTLASVLDCTVTPMGSRMLKRWLHMPVRDTRVLLERQQTIGALQDF
                     TAELQPVLRQVGDLERILARLALRTARPRDLARMRHAFQQLPELRAQLENVDSAPVQA
                     LREKMGEFAELRDLLERAIIDTPPVLVRDGGVIASGYNEELDEWRALADGATDYLERL
                     EVRERERTGLDTLKVGFNAVHGYYIQISRGQSHLAPINYMRRQTLKNAERYIIPELKE
                     YEDKVLTSKGKALALEKQLYEELFDLLLPHLEALQQSASALAELDVLVNLAERAYTLN
                     YTCPTFIDKPGIRITEGRHPVVEQVLNEPFIANPLNLSPQRRMLIITGPNMGGKSTYM
                     RQTALIALMAYIGSYVPAQKVEIGPIDRIFTRVGAADDLASGRSTFMVEMTETANILH
                     NATEYSLVLMDEIGRGTSTYDGLSLAWACAENLANKIKALTLFATHYFELTQLPEKME
                     GVANVHLDALEHGDTIAFMHSVQDGAASKSYGLAVAALAGVPKEVIKRARQKLRELES
                     ISPNAAATQVDGTQMSLLSVPEETSPAVEALENLDPDSLTPRQALEWIYRLKSLV"
     gene            10869..11213
                     /locus_tag="J1Y83_001479"
     CDS             10869..11213
                     /locus_tag="J1Y83_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrous oxide-stimulated promoter family
                     protein"
                     /protein_id="HBA7629636.1"
                     /translation="MSGKRISREKLTIKKMIDLYQAKCPQASAEPEHYEALFAYAQKR
                     LDKCVFGEEKPACKQCPVHCYQSAKREEMKQIMRWAGPRMLWRHPILTVRHLIDDKRP
                     VPELPEKYRPKK"
     gene            complement(11252..11680)
                     /locus_tag="J1Y83_001480"
     CDS             complement(11252..11680)
                     /locus_tag="J1Y83_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013364999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HBA7629637.1"
                     /translation="MAVSARNQLTGTVSAVAMGAVNDEVELTLAGGAKLVAIVTHSSQ
                     QALGLAKGKEAIALIKAPWVTLATEDCGLKFSARNQFAGSVSTITEGAVNATVHIKTD
                     AGFEIVAVVTNESQDEMKLTTGSRVIALIKASAILIATKA"
     gene            complement(11725..13803)
                     /gene="flhA"
                     /locus_tag="J1Y83_001481"
     CDS             complement(11725..13803)
                     /gene="flhA"
                     /locus_tag="J1Y83_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase transcriptional activator
                     FlhA"
                     /protein_id="HBA7629638.1"
                     /translation="MSYTPMSDLGQQGLFDITRTLLQQPDLASLCEALSQLVKRSALA
                     DNAAIVLWQAQTQRASYYASREKDTPIKYEDETVLAHGPVRSILSRPDTLHCSYEEFC
                     ETWPQLATGGLYPKFGHYCLMPLAAEGHIFGGCEFIRYDDRPWSEKEFNRLQTFTQIV
                     SVVTEQIQSRVVNNVDYELLCRERDNFRILVAITNAVLSRLDMDELVSEVAKEIHYYF
                     DIDDISIVLRSHRKNKLNIYSTHYLDKQHPAHEQSEVDEAGTLTERVFKSKEMLLINL
                     HERDDLAPYERMLFNTWGNQIQTLCLLPLMSGDTMLGVLKLAQCEEKVFTTTNLNLLR
                     QIAERVAIAVDNALAYQEIHRLKERLVDENLALTEQLNNVDSEFGEIIGRSEAMYSVL
                     KQVEMVAQSDSTVLILGETGTGKELIARAIHNLSGRNNRRMVKMNCAAMPAGLLESDL
                     FGHERGAFTGASAQRIGRFELADKSSLFLDEVGDMPLELQPKLLRVLQEQEFERLGSN
                     KIIQTDVRLIAATNRDLKKMVADREFRSDLYYRLNVFPIHLPPLRERPEDIPLLAKAF
                     TFKIARRLGRNIDSIPAETLRILSNMEWPGNVRELENVIERAVLLTRGNVLQLSLPDI
                     ALPEPETPPAATVVAQEGEDEYQLIVRVLKETNGVVAGPKGAAQRLGLKRTTLLSRMK
                     RLGIDKSALI"
     gene            complement(13877..14887)
                     /gene="hypE"
                     /locus_tag="J1Y83_001482"
     CDS             complement(13877..14887)
                     /gene="hypE"
                     /locus_tag="J1Y83_001482"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417210.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation carbamoyl dehydratase
                     HypE"
                     /protein_id="HBA7629639.1"
                     /translation="MNNIQLAHGSGGQAMQQLINSLFMEAFANPWLAEQEDQARLDLA
                     QLVAEGDRLAFSTDSYVIDPLFFPGGNIGKLAICGTANDVAVSGAIPRYLSCGFILEE
                     GLPMETLKAVVTSMAETARAAGIAIVTGDTKVVQRGAADKLFINTAGMGAIPANIHWG
                     AQTLTAGDVLLVSGTLGDHGATILNLREQLGLDGELVSDCAVLTPLIQTLRDIPGVKA
                     LRDATRGGVNAVVHEFAAACGCGIELSEAALPVKPAVRGVCELLGLDALNFANEGKLV
                     IAVERNAAEQVLAALHSHPLGKDAALIGEVVERKGVRLAGLYGVKRTLDLPHAEPLPR
                     IC"
     gene            complement(14884..16005)
                     /gene="hypD"
                     /locus_tag="J1Y83_001483"
     CDS             complement(14884..16005)
                     /gene="hypD"
                     /locus_tag="J1Y83_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase formation protein HypD"
                     /protein_id="HBA7629640.1"
                     /translation="MRFVDEYRAPEQVMQLIEHLRERASHLSYTAERPLRIMEVCGGH
                     THAIFKFGLDQLLPENVEFIHGPGCPVCVLPMGRIDTCVEIASHPEVIFCTFGDAMRV
                     PGKQGSLLQAKARGADVRIVYSPMDALKLAQENPTRKVVFFGLGFETTMPTTAITLQQ
                     AKARDVQNFYFFCQHITLIPTLRSLLEEPDNGIDAFLAPGHVSMVIGTDAYNFIASDF
                     HRPLVVAGFEPLDLLQGVVMLVEQKIAAHSKVENQYRRVVPDAGNLLAQQAIADVFCV
                     NGDSEWRGLGVIESSGVHLTPDYQRFDAEAHFRPAPQQVCDDPRARCGEVLTGKCKPH
                     QCPLFGNTCNPQTAFGALMVSSEGACAAWYQYRQQESEA"
     gene            complement(16005..16277)
                     /gene="hypC"
                     /locus_tag="J1Y83_001484"
     CDS             complement(16005..16277)
                     /gene="hypC"
                     /locus_tag="J1Y83_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 3 maturation protein HypC"
                     /protein_id="HBA7629641.1"
                     /translation="MCIGVPGQIRTIDGNQAKVDVCGIQRDVDLTLVGSCDENGQPRV
                     GQWVLVHVGFAMSVINEAEARDTLDALQNMFDVEPDVGALLYGEEK"
     gene            complement(16268..17140)
                     /gene="hypB"
                     /locus_tag="J1Y83_001485"
     CDS             complement(16268..17140)
                     /gene="hypB"
                     /locus_tag="J1Y83_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase nickel incorporation protein HypB"
                     /protein_id="HBA7629642.1"
                     /translation="MCTTCGCGEGNLYIEGDEHNPHSAFRSAPFAPAARPKMKITGIK
                     APEFTPSQTEEGDLHYGHGEAGTHAPGMSQRRMLEVEIDVLDKNNRLAERNRARFAAR
                     KQLVLNLVSSPGSGKTTLLTETLMRLKDSVPCAVIEGDQQTVNDAARIRATGTPAIQV
                     NTGKGCHLDAQMIADAAPRLPLDDNGILFIENVGNLVCPASFDLGEKHKVAVLSVTEG
                     EDKPLKYPHMFAAASLMLLNKVDLLPYLNFDVEKCIACAREVNPEIEIILISATSGEG
                     MDQWLNWLETQRCA"
     gene            complement(17144..17494)
                     /gene="hypA"
                     /locus_tag="J1Y83_001486"
     CDS             complement(17144..17494)
                     /gene="hypA"
                     /locus_tag="J1Y83_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HBA7629643.1"
                     /translation="MHEITLCQRALELIEQQAAKHGAKRVTGVWLKIGAFSCVETSSL
                     AFCFDLVCRGSVAEGCKLHLEEQEAECWCETCQQYVTLLTQRVRRCPLCHGDMLQIVA
                     DDGLQIRRIEIDQE"
     gene            17706..18167
                     /gene="hycA"
                     /locus_tag="J1Y83_001487"
     CDS             17706..18167
                     /gene="hycA"
                     /locus_tag="J1Y83_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase regulator HycA"
                     /protein_id="HBA7629644.1"
                     /translation="MTIWEISEKADYIAQRHRRLQDQWHIYCNSLVQGITLSKARLHH
                     AMSCAPDKELCFVLFEHFRIYVTLADGFNSHTIEYYVETKDGEDKQRIAQAQLSIDGM
                     IDGKVNIRDREQVLEHYLEKIAGVYDSLYTAIENNVPVNLSQLVKGQSPAA"
     gene            18292..18903
                     /locus_tag="J1Y83_001488"
     CDS             18292..18903
                     /locus_tag="J1Y83_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HBA7629645.1"
                     /translation="MNRFVIADSTLCIGCHTCEAACSETHRQHGLQSMPRLRVMLNEK
                     ESAPQLCHHCEDAPCATVCPVNAITRVDGAVQLNESLCVSCKLCGIACPFGAIEFSGS
                     RPLDIPANANTPKAPPAPPAPARVSTLLDWVPGIRAIAVKCDLCSFDEQGPACVRMCP
                     TKALHLVDNTDIARVSKRKRELTFNTDFGDLTLFQQAQSGEAK"
     gene            18900..20726
                     /gene="hycC"
                     /locus_tag="J1Y83_001489"
     CDS             18900..20726
                     /gene="hycC"
                     /locus_tag="J1Y83_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit 3"
                     /protein_id="HBA7629646.1"
                     /translation="MSAISLINSGVAWFVAVAVLAFLFSFQKALSGWIAGIGGAVGSL
                     YTAAAGFTVLTGTVGVSGALSLVSYDVQISPLNAIWLITLGLCGLFVSLYNIDWHRHA
                     QVKCNGLQINMLMAAAVCAVIASNLGMFVVMAEIMALCAVFLTSNSKEGKLWFALGRL
                     GTLLLAIACWLLWQRYGTLDLRLLDMRMQQLPLGSDIWLLGVIGFGLLAGIIPLHGWV
                     PQAHANASAPAAALFSTVVMKIGLLGILTLSLLGGNAPLWWGIALLVLGMITAFVGGL
                     YALMEHNIQRLLAYHTLENIGIILLGLGAGVTGIALEQPALIALGLVGGLYHLLNHSL
                     FKSVLFLGAGSVWFRTGHRDIEKLGGIGKKMPVISIAMLVGLMAMAALPPLNGFAGEW
                     VIYQSFFKLSNSGAFVARLLGPLLAVGLAITGALAVMCMAKVYGVTFLGAPRTKEAEN
                     ATCAPLLMSVSVVALAICCVIGGVAAPWLLPMLSAAVPLPLEPANTTVSQPMITLLLI
                     ACPLLPFIIMAICKGDRLPSRSRGAAWVCGYDHEKSMVITAHGFAMPVKQAFAPVLKL
                     RKWLNPVSLVPGWQCEGSALLFRRMALVELAVLVVIIVSRGA"
     gene            20729..21652
                     /gene="hycD"
                     /locus_tag="J1Y83_001490"
     CDS             20729..21652
                     /gene="hycD"
                     /locus_tag="J1Y83_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycD"
                     /protein_id="HBA7629647.1"
                     /translation="MSVLYPLIQALVLFAVAPLLSGITRVARARLHNRRGPGVLQEYR
                     DIIKLLGRQSVGPDASGWVFRLTPYVMVGVMLTIATALPVVTVGSPLPQLGDLITLLY
                     LFAIARFFFAISGLDTGSPFTAIGASREAMLGVLVEPMLLLGLWVAAQVAGSTNISNI
                     TDTVYHWPLSQSIPLVLALCACAFATFIEMGKLPFDLAEAEQELQEGPLSEYSGSGFG
                     VMKWGISLKQLVVLQTFVGVFIPWGQMETFTVGGLLLALVIAIVKLVVGVLVIALFEN
                     SMARLRLDITPRITWAGFGFAFLAFVSLLAA"
     gene            21670..23379
                     /locus_tag="J1Y83_001491"
     CDS             21670..23379
                     /locus_tag="J1Y83_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase large subunit"
                     /protein_id="HBA7629648.1"
                     /translation="MSEEKLGQHYLAALNEAFPGVVLDHAWQTKDQLTITVKVNYLPE
                     VVEFLYYKQGGWLSVLFGNDERKLNGHYAVYYVLSMEKGTKCWVTVRVEVDANKPEYP
                     SVTPRVPAAVWGEREVRDMYGLIPVGLPDERRLVLPDDWPDELYPLRKDSMDYRQRPA
                     PTTDAETYEFINELGDKKNNVVPIGPLHVTSDEPGHFRLFVDGENIIDADYRLFYVHR
                     GMEKLAETRMGYNEVTFLSDRVCGICGFAHSTAYTTSVENAMGIQVPERAQMIRAILL
                     EVERLHSHLLNLGLACHFTGFDSGFMQFFRVRETSMKMAEILTGARKTYGLNLIGGIR
                     RDLLKDDMIQTRQLAQQMRREVQELVDVLLSTPNMEQRTVGIGRLDPEIARDFSNVGP
                     MVRASGHARDTRADHPFVGYGLLPMEVHSEQGCDVISRLKVRINEVYTALNMIDYGLD
                     NLPGGPLMVEGFTYIPHRFALGFAEAPRGDDIHWSMTGDNQKLYRWRCRAATYANWPT
                     LRYMLRGNTVSDAPLIIGSLDPCYSCTDRMTVVDVRKKKSKVVPYKELERYSIERKNS
                     PLK"
     gene            23389..23931
                     /gene="hycF"
                     /locus_tag="J1Y83_001492"
     CDS             23389..23931
                     /gene="hycF"
                     /locus_tag="J1Y83_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycF"
                     /protein_id="HBA7629649.1"
                     /translation="MFTFIKKVIKTGTATSSYPLEPIAVDKNFRGKPEQNPQQCIGCA
                     ACVNACPSNALTVETDLATGELAWQFDLGRCIFCGRCEEVCPTAAIKLSQEYELAVWK
                     KEDFLQQSRFALCNCRVCNRPFAVQKEIDYAIALLKHNGDSRAENHRESFETCPECKR
                     QKCLVPSDRIELTRHMKEAI"
     gene            23931..24698
                     /gene="hycG"
                     /locus_tag="J1Y83_001493"
     CDS             23931..24698
                     /gene="hycG"
                     /locus_tag="J1Y83_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycG"
                     /protein_id="HBA7629650.1"
                     /translation="MSNLLGPRDANGIPVPMTVDESIASMKASLLKKIKRSAYVYRVD
                     CGGCNGCEIEIFATLSPLFDAERFGIKVVPSPRHADILLFTGAVTRAMRSPALRAWQS
                     APDPKICISYGACGNSGGIFHDLYCVWGGTDKIVPVDVYIPGCPPTPAATLYGFAMAL
                     GLLEQKIHARGPGEQDEQPAEILHGDMVQPLRVKVDREARRLAGYRYGRQIADDFLTQ
                     LGQGEEQVARWLEAENDPRLNEIVSHLNHVVEEARIR"
     gene            24695..25105
                     /locus_tag="J1Y83_001494"
     CDS             24695..25105
                     /locus_tag="J1Y83_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HycH family protein"
                     /protein_id="HBA7629651.1"
                     /translation="MSEKVVFSQLSRKFIDENDATPAEAQQVVYYSLAIGHHLGVIDC
                     LEAALTCPWDDYLAWIATLEAGSEARRKMEGVPKYGEIVIDINHVPMLANAFDKARAA
                     QTSQQKEWSTMLLSMLHDIHQENAIYLMVRRLRD"
     gene            25098..25565
                     /gene="hycI"
                     /locus_tag="J1Y83_001495"
     CDS             25098..25565
                     /gene="hycI"
                     /locus_tag="J1Y83_001495"
                     /EC_number="3.4.23.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation peptidase HycI"
                     /protein_id="HBA7629652.1"
                     /translation="MTDVLLCVGNSMMGDDGAGPLLAEKCAAAPKGNWVVIDGGSAPE
                     NDIVAIRELRPTRLLIVDATDMGLNPGEIRIIDPADIAEMFMMTTHNMPLNYLIDQLK
                     EDIGEVIFLGIQPDIVGFYYPMTQPIKDAVETVYQRLEGWEGNGGFAQLAVEE"
     gene            25801..26220
                     /locus_tag="J1Y83_001496"
     CDS             25801..26220
                     /locus_tag="J1Y83_001496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020078819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBA7629653.1"
                     /translation="MTANAARAVKATRELVNAVPFLGGSDSEDDYREALELVEYLIEE
                     DDTNPLIDFLASRIAEYENNNEKFAEFDKAVAAMPVGVALLRTLIDQHNLTYADLKNE
                     IGSKSLVSQILSGQRSLTISHIKALSARFGVKPEWFL"
     gene            complement(26318..27742)
                     /locus_tag="J1Y83_001497"
     CDS             complement(26318..27742)
                     /locus_tag="J1Y83_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HBA7629654.1"
                     /translation="MSVFPQGFLWGGALAANQSEGAYREGGKGLTTVDMIPHGEHRMA
                     VKLGLEKRFQLRDDEFYPSHEATDFYHRYKEDIALMAEMGFKVFRTSIAWSRLFPQGD
                     ELTPNQQGIAFYRAVFEECKKYGIEPLVTLCHFDVPMHLVTEYGSWRNRKLVELFSRY
                     ARTCFEAFDGLVKYWLTFNEINIMLHSPFSGAGLVFEEGENQDQMKYQAAHHQLVASA
                     LATKIAHEVNPQNQVGCMLAGGNFYPYSCKPEDIWAALEKDRENLFFIDVQARGAYPA
                     YSARVFREKGVTIDKAPSDDEILKNTVDFVSFSYYASRCASAEMNANNCSAANVVKSL
                     RNPYLQVSDWGWGIDPLGLRITMNMMYDRYQKPLFLVENGLGAKDEFAANGEINDDYR
                     ISYLREHIRAMGEAIADGIPLMGYTTWGCIDLVSASTGEMSKRYGFVYVDRDDAGNGT
                     LTRTRKKSFWWYKKVIASNGEDLE"
     gene            complement(27751..29208)
                     /gene="ascF"
                     /locus_tag="J1Y83_001498"
     CDS             complement(27751..29208)
                     /gene="ascF"
                     /locus_tag="J1Y83_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose/arbutin/salicin transporter
                     subunit IIBC"
                     /protein_id="HBA7629655.1"
                     /translation="MAKNYAALASSVITALGGVDNISAVTHCMTRLRFVIKDDQLIDS
                     PTLKTISGVLGVVRSDNQCQVIIGNTVSLAFQEVVSLLPGDMQPAQPVGKPKLTLRRI
                     GAGILDALIGTMSPLIPAIIGGSMVKLLAMILEMSGVLTKGSPTLTILNVIGDGAFFF
                     LPLMVAASAAIKFKTNMSLAIAIAGVLVHPSFIELMAKAAQGEHVEFALIPVTAVKYT
                     YTVIPALVMTWCLSYIERWVNSITPAVTKNFLKPMLIVLIAAPLAILLIGPIGIWIGS
                     AISALVYTIHGYLGWLSVAIMGALWPLLVMTGMHRVFTPTIIQTIAETGKEGMVMPSE
                     IGANLSLGGSSLAVAWKTKNPELRQTALAAAASAIMAGISEPALYGVAIRLKRPLIAS
                     LISGFICGAVAGMAGLASHSMAAPGLFTSVQFFDPANPMSIVWVFAVMALAVVLSFIL
                     TLLLGFEDIPVEEATAEARKHQSVQPTVAKEVSLN"
     gene            29468..30478
                     /locus_tag="J1Y83_001499"
     CDS             29468..30478
                     /locus_tag="J1Y83_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBA7629656.1"
                     /translation="MTTMLEVAKRAGVSKATVSRVLSGNGYVSQETKDRVFQAVEESG
                     YRPNLLARNLSAKSTQTLGLVVTNTLYHGIYFSELLFHAARMAEEKGRQLLLADGKHS
                     AEEERQAIQYLLDLRCDAIMIYPRFLSVDEIDDIIDAHSQPIMVLNRRLRKNSSHSVW
                     CDHKQTSFNAVAELITAGHQEIAFLTGSMDSPTSIERLAGYKDALAQHGIAFNEKLIA
                     NGKWTPASGAEGVETLLERGAKFSALVASNDDMAIGAIKALHERGVAVPEQVSVIGFD
                     DIAIAPYIVPALSSVKIPVTEMIQEIIGRLIFMLDGGDFSPPKTFSGKLIRRGSLIAL
                     SR"
     gene            30627..31154
                     /gene="hydN"
                     /locus_tag="J1Y83_001500"
     CDS             30627..31154
                     /gene="hydN"
                     /locus_tag="J1Y83_001500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport protein HydN"
                     /protein_id="HBA7629657.1"
                     /translation="MNRFIIADASKCIGCRTCEVACVVSHQENQDCASLTPETFLPRI
                     HVIKGVNISTATVCRQCEDAPCANVCPNGAISRDKGFVHVMQERCIGCKTCVVACPYG
                     AMEVVVRPVIRNSGAGLNVRADKAEANKCDLCNHREDGPACMAACPTHALICVDRNKL
                     EQLSAEKRRRTALMF"
     gene            31307..33559
                     /gene="hypF"
                     /locus_tag="J1Y83_001501"
     CDS             31307..33559
                     /gene="hypF"
                     /locus_tag="J1Y83_001501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase HypF"
                     /protein_id="HBA7629658.1"
                     /translation="MAKNTSCGVQLRIRGKVQGVGFRPFVWQLAQQLNLHGDVCNDGD
                     GVEVRLLEDPETFLVQLHQHCPPLARIDSVEREPFIWSQLPTEFTIRQSAGGVMNTQI
                     VPDAATCPACLAEMNTPGERRWRYPFINCTHCGPRFTIIRAMPYDRPFTVMAAFPLCP
                     ACDKEYCDPLDRRFHAQPVACPECGPHLEWVSHGEHAEQEAALQAAIAQLKMGNIVAI
                     KGIGGFHLACDARNSNAVATLRARKHRPAKPLAVMLPVADGLPDAARQLLTTPAAPIV
                     LVDKKYVPELCDDIAPDLNEVGVMLPANPLQHLLLQELQCPLVMTSGNLSGKPPAISN
                     EQALADLQGIADGFLIHNRDIVQRMDDSVVRESGEMLRRSRGYVPDALALPPGFKNVP
                     PVLCLGADLKNTFCLVRGEQAVLSQHLGDLSDDGIQMQWREALRLMQNIYDFTPQYVV
                     HDAHPGYVSSQWAREMNLPTQTVLHHHAHAAACLAEHLWPLDGGDVIALTLDGIGMGE
                     NGALWGGECLRVNYRECQHLGGLPAVALPGGDLAAKQPWRNLLAQCLRFVPEWQNYSE
                     TASVQQQNWSVLARAIERGINAPLASSCGRLFDAVAAALGCAPATLSYEGEAACALEA
                     LEASCHGVTHPVTMPLVDNQLDLATFWQQWLSWQAPVNQRAWAFHDALAQGFAALMRE
                     QATMRGITTLVFSGGVIHNRLLRARLAHYLADFTLLFPQSLPAGDGGLSLGQGVIVAA
                     RWLAGEVQNG"
BASE COUNT         7752 a         8812 c         8995 g         8022 t
ORIGIN      
        1 aacgcattaa taaaggacta cgtatgcaag gcattaaaat atatacaatt ctagcctctt
       61 ctcttatatc tggtagtatt tttgctcaaa cggaaatttc gactgcaaat tcgtctacag
      121 ttaatgcaag tcatgttgaa ccttcagcgg aaaaaataat atcgccaagc gataaactca
      181 ataacctttt tgaaagaaat atgtctcagc cttatatttt acaaaaaatt ggcgagagaa
      241 cttattatgt tcaacgctat ttttattcca ctacgtttta tgtcggagac aaaggggttt
      301 tgctgtttga tgctccggag ggtcgcggta aatatttatt acaggcaatt cgtgatgtga
      361 ctccattacc cgtaacagcg ctggtttatt ctcattacca tgtggatcac attggcgata
      421 gtccattttg gaacgatgag gcgaagaaag aaggcgtgaa tctgcgtatt attgccagta
      481 aagccactgc tgaaaaaatg caatttatga acagtcgctt acccgtcgcc actcaggttt
      541 taagtaaaaa agatgaccag tttaaatttg aaaagcagac cattgaatta caccgatttg
      601 ttaaagcagg acataccgac gaccattctg tatggttgct aaaacaggaa aaagtagctc
      661 acagcccgga tctcctgaat cctgaccaac tgccgatgat gggcttcgcc gtatcggata
      721 cgttggttta ccatgacagt aatttgcgtc aggttgaaat gcttgactgg aaatatttta
      781 ttggcgggca tggtaatatt ggtttacacg atgatttcaa atttcaacgc cagtttctta
      841 atgatcttag ggatacgaca attaaagtca gaaaagaaga aagttttggt aaatttatga
      901 ataaaacggc aaataatcat gctgattttg cgcgagccca gcgcgaagca attattaaga
      961 aagtaactga agtattacgt ccgaaatatg gccatatgta tggctacgac gcatctatgc
     1021 ctgctaatat cgaaatggca ataagattgg tcggttctta ttactaagat aaaatcaact
     1081 tgcaagagac tgtaattata ttgcaaagtg aaaattaacc tcggtagcga gatattaccg
     1141 aggttaatat tggcatacat tacaccctta tcaggcactt aaacgataca tcatatctta
     1201 tctggattat tagatgacga gccatacgca ccaggtaatt aagaatccgg taaaaccgag
     1261 aatggtcgtt aacactgtcc aggttttcag accgtctgct accgacaacc ccagatattt
     1321 ggtcacaatc cagaaccctg agtcattaat atgtgacgcg ccaagcccac caaagcaggc
     1381 tgccagcgtc accaatacgc actgaatcgg atttaatccc atcaccgctt ctgagagtaa
     1441 cccgccggtt gtcaggattg ctacggttgc tgacccctgc gatgcacgca gcgccagtga
     1501 aataataaat gcggctggta acagaggcag gtcaatcatt tgtaacatgt tggcaagggc
     1561 tttaccgatg cccgattcca ccagcacttt gccaaatacc cctccagcac cagtaaccaa
     1621 aatcactacc gccgcagtgg gaagggcaga gcccataatg tcgctggtgt gttgtaagct
     1681 ccagccgcga cgtaaagcca ataaccagaa tgccagcacc agcgcaatca ttagagctac
     1741 cattggtgag ccgatcaact gtagcgtacc aagcagggga tgcgaaggcg gcatcagtgt
     1801 tgcagaaacc gtacccgcca tgataatcgc gataggaata acaattagcg aggtgaccag
     1861 cgcgacgccc ggtggattta ttttatcgct taattttgtc gcgccttcct cactggccgg
     1921 agccagttgc atctgttcca gtacctcgac cgacatcgca tattggcgct tattgattat
     1981 tttcgctgca aagtagccaa caatccctac gggaatagaa atcgcaatac cgatgatggt
     2041 tagccagccg atgtctgcgt ggagtaaccc cgctgcggcg acagggcctg gatgcggcgg
     2101 taccgccacg tgaacagtga gcatgatccc agcgacaggc aggccaaatt tgagtggtga
     2161 tattttggca accttggcaa aaccgtaaat gattggcgca agaataataa agccgacatc
     2221 aaagaagacg ggaataccga ggaagaacgc tgccagagtc agcgcagcga tagttcgttt
     2281 gtcacctaac ttgcgactga aataattagc cagtgactct gcaccaccag agtgttcgat
     2341 catacgcccc agcatagcgc ccagaccaat aataatagtg acggaaccaa gcacaccgcc
     2401 catcccggcg atcatcactt tacccacttc gcccgccggt atacccgccg caagtgcgac
     2461 taacaggctg acgaggagca gagcaacgaa tggttgtacc tttgccttga tgaccagtag
     2521 caacagcatg attacgccag ctaacgcaat gcataacaat gtaattgtgg acatgggaaa
     2581 ccctgtctga aagttatagt taacctaccc catccgtaga tggggggatg tatgggtacg
     2641 ttgtaattag ggattgagcg aattagcgcc aggcgtcaaa ccagccaagc ccttcttcgg
     2701 tgaggccacg aggtttgtat tcacaaccga tccagccctg gtatcccact tcatcgaaca
     2761 ggcggaacag ccacggatag ttgatttctc catcgtccgg ttcatgtcga tcaggtagtc
     2821 cggcaatttg tacgtgcgca tatttcccgg cgtagtcgcg gattaaatgc gtcaggttgc
     2881 catctacttt ttgcgcatgg aaagtatcta gttgaataaa cacgttatct cgcgcaacct
     2941 cttcaacaat agccagtgcc tgatactggc tggagaagag ataatgaggc ttaacgccgg
     3001 ggctgagtgc ttcaaccaat attcgcttgc cgtgtggcgc aaagcggtcg gcagcgtagc
     3061 ggagattatc aataaatact gcccggtacc gttcagcatc ttcgccagcg ggcacgacgc
     3121 ctgccatcac atggacttgt tcacaattga gcgccaatgc atattccagt gccaggtcga
     3181 tgtctgcgcg tgcttcgtgc tcacgtccgg gaagggcgga taatccccat tctcccgcat
     3241 taatatctcc gggagcggta ttgaacagcg ccagtgtcag atggttttgc accagttgct
     3301 gctggatttg ctgggtggag tagtcatagg gaaacagaaa ctccacagca tcgaacccgg
     3361 cttttcgcgc tgcggcgaag cgttcaataa aaggcacttc ggtgaacatc atggataaat
     3421 tagctgcaaa acgaggcatt gcattaactc cttaattccg caatttcacc tgcggtcaga
     3481 taacggatcg ggcggtcacc gagaataaaa atcagctttg ccgtttcctc cagctcttcc
     3541 atattgttgg cggcttcttg caggctttca ccgcaaacca ctgggccatg atttgccagt
     3601 aaaaaagcct gattgtctgc tgccagttcc gccagatcct gtgcgatgcg tttatcgccc
     3661 ggtcggtaat aaggcaccag cgggatattt cccatccgca tcaccacgta tggtgtgaac
     3721 ggacgaataa cgttgctgct gtccagccct tgcaggcagg aaagcgccgt cgaccacgta
     3781 ctgtgcaaat gcactaccgc tttacagcgc ggattgttgc gatacagcgc cagatgaaag
     3841 agcacctctt tcgagggttt gtcaccactt aaccattcgc catccgcggt gactttggaa
     3901 agccgctgcg gatcgagatt gcccaggcat gaacctgtcg gtgtcgccag taaattcccg
     3961 tcaggtaaaa gcagcgacag attgccagcc gaaccggttg catatccgcg ctgaaagaat
     4021 gaactggcaa tccgcgtcat ctcctctcgc aaagactgct ctacttttgc gaaatcgctc
     4081 atgataaaaa ctctctttgg gctcgtgaaa aaaaggcttc atcaccgaag ttgccagatt
     4141 taagggcgag tgagacaggc ttatccagtg cgtttaccca cggcacgccg ggggaaatgg
     4201 ttgggccaat atgaaaacct tttatgccca ggctctgtgt gactacgccg gaggtctcac
     4261 cgcctgcgac aataaagcgt gtcacgcctt ccgctgctaa ccgcgccgct agttgagaaa
     4321 acagtgtttc cactgcctga ctggcttttt gtgcaccgta ttgctgttga attgctgcca
     4381 atgcgtcagt gctggcggtg gcaaaaacca gtggagcaag tacactttcc tggcccagaa
     4441 cccactctgc cagctcgtgt gcataagcgg ccagagtttc ggttgagagg cagcgtgcca
     4501 catcaacctc acgggctggt gcaatttgac ggtaatgtgc cacctggcgg ttggtcattt
     4561 gagagcatga accggagagc actacgccgc gcccagcgag cggacgccct gcttcgcgag
     4621 cctggttacc gttttcttgc gcccactgcc gggccaggcc aatcgccaga ccagaaccgc
     4681 ccgttaccag tggggcatcg cgcaaggctt ccccctgaat ttccagatgg tgttcggtca
     4741 gcgcatcaag caccgcgtag cgatagccct cttgctgtaa gcgagccagc tcctgacgaa
     4801 cggctttcac accttgttcg aaaacatgtg ctgaaacgac gccgcagcgc cctgtggatt
     4861 gcgcttcaac cagacgggga agatagctgt cggtcatggg atttaccggg tgatggcgca
     4921 ttccggattc ggccagcagt tgattcatta cgaacaaata cccctgataa accgtacgtc
     4981 cgttgactgg cagggccgga gagaagaccg taaacggtgt gttgagagca tccattaatg
     5041 catcggtaac tgggccgata ttccctttcg ccgtactgtc gaaagtagag cagtatttga
     5101 aatagatctg tttgcaacct tgctgttgca accagctcag agccgccagc gattgctgtg
     5161 tggcttcaac caccggacag gagcgcgttt tcaggctgat caccagtgcg tcgattgctt
     5221 ccggcatttt acctgttgga acaccgttaa tttgtaccgt tggtagaccg ttttccacca
     5281 gaaaactggc gatatccgtc gcgccggtaa aatcatcggc gataacgcca atcttgatca
     5341 tgatttcgct cccggtaggg tgatgccaga gaaaatcttg ataactgcgc tatcgtcttc
     5401 tttcccgtaa cccgcgttac tggcgctggt gaacatattc aatgctgttg aggccaacgg
     5461 cagcgggaag tgcagggctt tggcggtatc ggcaaccaga ccaagatcct taacaaaaat
     5521 atcgacggct gaatgcgggg tgtaatcgcc atccaccaca tgacgcatcc ggttttcgaa
     5581 catccatgaa tttccggcgg cattggtcac gacgtcatac atcacatcca gcgggatccc
     5641 cgcacgggct gcaagcgcca tcgcttcggc tccggcagca atatgtacac ccgctaacaa
     5701 ctggtggata atttttacgg tcgaacccag tcccggttct gctccgatgc gataaacttt
     5761 tccggcaacg gcttccagca cgggtgctag tcgttcaaag gcaatatcgc taccggaggc
     5821 catgacagtc atttcaccgt tagcggcttt taccgcacca ccagaaaccg gcgcatccag
     5881 catttccaga ccgaatccag ccagagcggt agcaatttct tgcgcatcag cactggcgat
     5941 agtggaagaa accattactg ccgtaccggg tttcagatgt tgtgcaacgc ctttttcacc
     6001 aaacagcacc tgtttaacct gggtcgcatt gaccaccagc accagcagag cgtccagttt
     6061 ttcggcaaac gtcgcggcgt tatcagaaac tccgcaagca ccagcctctt tcaacgtagc
     6121 gcaggcattg ctgttcaggt ctgcgcccca ggtagaaaga tctgcgcgga cacatgacag
     6181 tgctgctccc attcccattg accctaagcc aacgataccg acatgaaact ccgatcccgt
     6241 tttcatctgc tctccttgtt aatttaagtg atattttgtt tgatgttgtg aatataagcg
     6301 cggaaagata acgatatggt gagctgattc acaaaaatta acattgtgtg ttattttatg
     6361 tgaactaagc gttagttgcg ccgcgcacgt ttcgcaggca aatagcgtag aatgtccgca
     6421 ggacaaagga aggagcaaaa gttgataccc gtagagcgtc gccaaatcat ccttgagatg
     6481 gtagctgaaa aaggcattgt cagtattgct gaactaacgg acagaatgaa tgtgtcacat
     6541 atgactattc gtcgggattt acaaaaactg gagcagcagg gagccgttgt gctggtgtcc
     6601 ggaggcgtcc agtctccggg acgcgtggcg catgaacctt ctcatcaggt aaaaactgcg
     6661 ctggcaatga cgcaaaaagc ggctattggc aagctggcgg caagtcttgt tcagccggga
     6721 cgttgtatct atctggatgc gggaacgacc acgttagcga ttgcacagca tctgattcac
     6781 atggagccac tcacagtggt tactaacgac ttcgttattg cggactactt gctcgacaac
     6841 agtaattgca caattattca cactggcggc gcagtgtgtc gggaaaaccg ttcctgtgtt
     6901 ggggaagccg ctgcgaccat gctgcgtagc ctgatgattg atcaggcttt tatttctgca
     6961 tcgtcatgga gtgtgcgggg gatctctacg ccagcagaag ataaagtcac ggtgaaacgg
     7021 gcgattgcca gtgccagccg ccagcgagtt ttggtctgtg atgcgacgaa atatggacag
     7081 gtggcgacat ggctggcgtt accattaagc gagtttgatc agatcattac tgacgacggt
     7141 ctgccggaga gtgccagccg cgcgctggcg aagctggatc tctctttgct ggtagcgaaa
     7201 aatgaataat gacctgcaat aacgccgggt tactcatgct tcacagaaga agcatgagac
     7261 tactttattt tataaaatga cagccgcccg cttttcggcg atccggtatc tatataaatc
     7321 tggttagcga acgtctgaat gttatcaaac atcatatgac caaatataaa gaagtcggca
     7381 ccgtttattt tttgtaattc gctattaagt gatttctgca cacgatcaac aggccagagt
     7441 aattcgctct ccgctatttc tttgccaaac tgatactcct tccccggata atctgcatgt
     7501 gcgatgacat attttatggt gtcgttagtg atttcaataa tatgtggaag gtgatggaat
     7561 ttcagcaaca gatctattgc ctcttgttgc tctgaatcat ttaaatcgaa aaaccagtca
     7621 ccaccgctgg caagccacat atttccatcg ccagtttcga atgcatcaag cgccatcgct
     7681 tcgtggttgc ctttgacgga gataaaccag ggctggttta acaggcgcag cacgttaaga
     7741 ctctccggtc cacgatcgat gttatcgcct accgaaataa gtaagtcggt ttcaggacaa
     7801 aaagagagtt gatgtaagcg ggattgtaat aattgatagt caccatgaat atcaccaacg
     7861 gcccatatat ggcgatagtg atgggcattg attttttgat agcgtgtaga tggcatggtt
     7921 ttaccctgta aaataagttt tcctattata cagggtattt ttatttgatt cgtcagttgt
     7981 cgttaatatt cccgatagca aaaggctatc gggaattatt attacaccag actcttcaag
     8041 cgataaatcc actccagcgc ctgacgcgga gtcaatgaat ccgggtcgag gttttccagt
     8101 gcctcgaccg caggcgaagt ttcttccggt acggacagca aagacatttg tgtaccatcc
     8161 acttgcgtag cagcggcgtt cggcgaaatg ctttccagct cacgcagttt ttgccgtgcg
     8221 cgcttaataa cctcttttgg cacacccgcc agagctgcaa ccgccaggcc gtagcttttg
     8281 ctcgctgcgc catcctgcac gctatgcata aaggcaatgg tgtcgccgtg ctccagtgca
     8341 tcgagatgca cgttggcgac gccttccatt ttctccggta actgggtcag ctcgaaatag
     8401 tgggtagcaa acagcgtcaa cgctttgatc ttatttgcca gattttccgc acatgcccac
     8461 gccagcgaca gaccatcgta agtggacgtt ccacgcccta tctcatccat caacaccaga
     8521 ctatattcgg tggcgttatg taaaatattg gcggtttcgg tcatctccac cataaaggtt
     8581 gaacgcccgg aagccagatc atccgcagca cctacgcggg taaagatacg gtcaatcggg
     8641 ccaatctcga ctttttgcgc cggtacgtag ctgccgatat acgccatcag cgcaatcaac
     8701 gcggtctggc gcatataggt acttttaccg cccatgttcg gaccggtaat gatcaacatg
     8761 cgacgctgcg gcgacaggtt cagcgggtta gcgataaacg gctcattcag tacttgttca
     8821 actaccggat ggcgaccttc ggtaatgcga atgccaggtt tatcaataaa ggtcgggcag
     8881 gtgtagttca gggtataggc ccgctccgcc aggttcacca gcacgtcgag ttccgccagc
     8941 gcgctcgcgc tctgttgcaa cgcttccaga tgcggcaaca gcaggtcgaa cagctcttca
     9001 taaagttgtt tttccagagc cagtgctttg ccttttgagg tgagaacttt atcttcgtac
     9061 tctttcagct ccggaataat gtaacgctcg gcgtttttca gcgtctggcg gcgcatgtag
     9121 ttaatcggtg ccagatggct ttgcccacgg ctgatctgaa tgtagtagcc gtgcaccgca
     9181 ttaaagccaa ctttcagcgt gtccaggccg gtacgttcac gctcgcggac ctccagacgc
     9241 tccagataat cggtcgcgcc gtcagccagc gcgcgccact catccagctc ttcgttatag
     9301 cccgatgcga taacaccacc gtcgcgtacc agcaccggtg gtgtgtcgat gattgctcgc
     9361 tccagcagat cgcgcagctc ggcaaactcg cccatcttct cacgcagcgc ttgtaccggt
     9421 gcactatcga cattttctaa ctgcgcacgc aactctggca gttgctggaa agcgtgacgc
     9481 atacgggcca gatcgcgtgg gcgagcggta cgcaacgcca gacgcgccag aatacgttcc
     9541 aggtcgccca cctgacgcag taccggctgt aactcggcgg tgaaatcctg caatgcgcca
     9601 atagtttgct ggcgctcaag caacacgcgg gtatcgcgca ctggcatatg cagccagcgt
     9661 ttcagcatac gactacccat cggcgttaca gtgcagtcga gcactgaagc cagcgtattt
     9721 tccgcaccgc cggccaggtt ctgagtaatt tccaggttac gacgcgtcgc ggcatccata
     9781 atgatgctgt cctgctgacg ttccatagtg atagaacgaa tatgcggcag ggtcgtgcgt
     9841 tgggtatctt tcgcatactg caacagacaa ccggcagcac aaagtccgcg tggtgcgttc
     9901 tccacaccaa aaccgaccag atctcgggtg ccaaattgca ggttcaattg ctggcgagcg
     9961 gtgtcgattt caaactccca cagcgggcga cgacgcaggc cgcggcgacc ttcaatcagc
    10021 gacatctcgg cgaaatcttc cgcatacagt aattccgctg gattcgtacg ttgcagttct
    10081 gccgccatcg tttcgcggtc cgccggttcg ctcaggcgaa aacgaccgga actgatatcc
    10141 agcgttgcgt agccgaaacc tttactgtcc tgccagatag ccgccagcag gttgtcctga
    10201 cgttcctgca acagcgcttc atcgctgatg gtgcccggcg taacgatacg gacaactttg
    10261 cgctcaaccg gccctttgct ggtcgccgga tcgccaatct gttcacaaat agcgacggat
    10321 tcgccctgat tcaccagttt ggcgaggtag ttttccaccg catggtaggg aatccccgcc
    10381 atcgggattg gctctcccgc tgaagcaccg cgtttggtca gtgaaatatc cagcagttgc
    10441 gacgcccgtt ttgcgtcgtc ataaaacagt tcataaaagt cgcccatccg gtaaaacagc
    10501 aggatctcgg gatgctgggc tttcagcttg agatactgct gcatcatggg cgtatgggcg
    10561 tcgaaatttt ctattgtact catggggtta agtccggctc cctgatgtta aatgtagtgt
    10621 gatggttttt attttcgttg taatcacata aggcgcacat gataccggag ttaatcattt
    10681 caggggaaga cgcgaaaggt gagttcttga tgtcggttcc gagaaaaatg ctgctttgca
    10741 gcttttgcgt gatgacgatg aaacaagatc gccaaattcg tcggttgaag aaacctcctc
    10801 cttcagagcc atagtcccct ttaaaattaa gttgtaatat aaatgcacct taagagtatg
    10861 gaggcagcat gtccggcaag cgtatctctc gtgaaaaact gacgattaaa aaaatgatcg
    10921 atctttatca agcgaaatgc ccgcaggcgt cagcagagcc ggagcattac gaggcgttgt
    10981 ttgcttacgc gcaaaagcgg ctggataaat gtgtgttcgg cgaagagaaa ccagcctgta
    11041 agcagtgtcc ggtgcactgt taccagtctg caaagcgtga agagatgaaa cagattatgc
    11101 gttgggccgg gccgagaatg ctgtggcgtc atccgatctt aaccgtgcgt catctcatcg
    11161 atgacaagcg tccggtgccg gagttgccag aaaaatatcg tccaaagaaa taaatctgcc
    11221 ggagagccat actctccggc aaatacggcg cttacgcttt ggtggcaatc aggatggctg
    11281 atgctttgat caatgcaata acgcgcgagc cagtggtgag tttcatttca tcctgacttt
    11341 catttgttac cactgccaca atttcaaaac ccgcatccgt tttgatatgg acagtggcgt
    11401 tcactgcgcc ttcggtgatt gtcgacacgc tgccagcaaa ctggttgcga gcagagaatt
    11461 tcagtccaca gtcttccgtt gccagcgtta cccacggggc tttaatcagg gcaatcgctt
    11521 ctttcccttt cgccagaccc agcgcttgct ggctgctgtg tgtaacaatt gccaccagct
    11581 ttgcaccacc tgccagcgtc agttctactt catcattcac agcgcccatt gctactgcgc
    11641 taactgttcc ggttaattga tttcttgctg aaactgccat actgcctcct ttcattgaat
    11701 gttgatcgag tataaccaca tctgttaaat caatgccgat ttatcaattc ccagtcgctt
    11761 catccgtgac agcagagtcg tgcgtttcag ccccagacgt tgcgcagcgc ctttaggccc
    11821 ggcaaccacg ccgtttgttt ctttcagcac gcgaacaatc aactgatatt catcttcgcc
    11881 ctcctgggcg acaaccgttg cggcaggcgg cgtttcaggt tctggtaaag caatatctgg
    11941 caatgacagc tgcagcacgt tgccgcgtgt tagcaatacc gcgcgctcaa tgacgttttc
    12001 cagctcacgc acgttgcccg gccattccat gttgctcagg atgcgcagcg tctcggcagg
    12061 aatgctgtcg atattgcgcc ccagacgacg ggcaattttg aaggtaaagg ctttcgccag
    12121 cagcggaata tcttccggac gctcgcgtag tggcggcagg tggatcggga atacgttcag
    12181 gcggtaatag agatcgctac ggaactctcg gtcggcgacc atttttttca gatcgcggtt
    12241 agtcgcggca attaaacgta cgtccgtctg aatgattttg ttgctgccga gacgttcaaa
    12301 ctcctgttcc tgtaatacac gcagcaactt cggctgtaac tccagcggca tatcgcccac
    12361 ttcatcgagg aatagtgagc ttttatccgc cagttcaaaa cgaccaatgc gctgagcgct
    12421 ggcaccggta aaagccccac gctcatgacc aaatagatcg ctctccagca atccggcagg
    12481 catcgccgcg cagttcattt tgaccatgcg gcgattatta cgcccactga gattatggat
    12541 cgcgcgggca atcagctctt tacctgtacc agtttcaccg aggatcagaa cggtactgtc
    12601 actttgcgcc accatttcca cttgtttaag tacgctgtac atggcttcgc tgcggccaat
    12661 aatctcgcca aattcactat caacattgtt gagctgctcg gtcagggcga ggttttcatc
    12721 aaccagccgt tctttcagac gatgaatttc ctgataggcg agggcgttat cgacagcgat
    12781 tgccacacgt tcggcaatct ggcgcagtaa attcagattg gtagtggtaa agactttctc
    12841 ttcgcattgc gccagtttca gtacgcccag catggtgtcg ccagacatca gcggtaacag
    12901 gcacaaggtt tgaatctgat tgccccaggt gttgaacaac atgcgttcat agggggctaa
    12961 atcgtcccgc tcgtggagat taatcaacag catctcttta cttttgaaga cgcgttcggt
    13021 gagggttccg gcttcatcga cttcgctctg ttcgtgagcg ggatgctgtt tatcaagata
    13081 gtgagtggag tagatgttga gtttgttctt acggtggctg cgtaagacga tgctgatatc
    13141 gtcaatgtcg aagtagtagt ggatttcttt ggcgacttcg ctgaccagtt cgtccatatc
    13201 caggcgggaa agcaccgcgt tagtgatggc gaccaggatg cggaagttat cgcgttcccg
    13261 gcataacaac tcatagtcga cattgttaac gacgcggctc tggatttgtt cggtgacgac
    13321 agaaacgatc tgcgtaaatg tttgcagacg attgaactct ttttcgctcc aggggcgatc
    13381 gtcataacga ataaattcac agccaccaaa aatatgccct tccgccgcca gtggcatcag
    13441 gcaatagtga ccaaatttgg ggtatagccc acctgtggcc agctgcggcc aggtttcaca
    13501 aaattcttcg taactgcaat gcagcgtatc agggcgcgac aaaatgctgc gtaccggacc
    13561 gtgtgccaga acagtttcgt cttcatattt aatgggggtg tctttttcac gcgatgcgta
    13621 ataagacgca cgttgagtct gcgcttgcca caacacaatc gccgcgttgt cggcgagcgc
    13681 agaacgcttt accagttgcg aaagagcctc acacagcgag gccagatcgg gctgctgcaa
    13741 tagtgtccga gtgatgtcga acaacccttg ctgtccgaga tcactcatcg gtgtatatga
    13801 cattttgttc ttccaggaaa gcaccgcaaa gcggtgcaaa aggtcattga ctaactatag
    13861 gagatttaga attttattag catatacgcg gaagcggttc ggcgtgcggt agatcgaggg
    13921 ttcgtttcac gccatacaga ccggcaagac gaacaccttt acgttctact acttcgccaa
    13981 tcagcgccgc atcttttccc agtggatggg aatgtaacgc cgccagcact tgctctgctg
    14041 cgttgcgttc aacggcgatc accagtttgc cttcgttggc aaagttaagg gcgtccagtc
    14101 ccagcagttc gcaaacgccg cgcacagcag gtttaaccgg cagtgccgct tctgaaagtt
    14161 caataccaca accgcaggct gccgcgaact catgaaccac cgcgtttaca ccaccacggg
    14221 tggcatcacg cagcgctttc acgccgggaa tgtcacgcag cgtctgaata agcggcgtca
    14281 gcaccgcgca gtcgctgacc agttctccat ccagccctag ttgctcacgc aggttaagga
    14341 tagtcgcccc atggtcgccg agtgtaccgc taaccagcaa tacatcgcct gcggttagcg
    14401 tctgtgcgcc ccagtgaata ttcgccggaa ttgcgcccat gccagcggtg ttgataaaca
    14461 gtttatctgc cgcgccgcgc tgtaccactt tggtatcgcc agtaacgatg gcaatgcctg
    14521 ccgcgcgggc ggtttctgcc atgctggtca ctacggcttt cagtgtctcc atcggcaatc
    14581 cttcttcgag gataaagcca caggagagat agcgcggaat tgcgccactg accgcaacgt
    14641 cattggcggt gccgcaaatc gccagcttgc cgatattacc gccaggaaag aacagcgggt
    14701 caatgacgta actgtcggtg gagaacgcca gacggtcgcc ttccgctacc agctgtgcca
    14761 aatcaagacg ggcctgatct tcctgctctg ccagccacgg gttggcaaag gcttccataa
    14821 acaggctgtt gattaattgc tgcatcgcct ggccgccgct accgtgggcg agttggatat
    14881 tattcacgct tcactctcct gctgacgata ctgataccac gcagcgcacg ctccttcgga
    14941 ggaaaccatc agcgcaccaa acgcggtttg aggattacag gtgttaccaa acagcgggca
    15001 ttgatgcggc ttacatttgc ccgttaatac ttcaccacaa cgcgcacgcg ggtcatcgca
    15061 gacctgctgc ggtgccgggc ggaaatgtgc ttcggcatcg aatcgttgat aatccggcgt
    15121 caggtgcacg ccagaagatt caatcacgcc taagccgcgc cattcactgt cgccgttgac
    15181 acagaacaca tcagcaattg cctgttgcgc cagcaggtta ccggcatccg gcaccacccg
    15241 acgatactga ttctctacct tgctatgggc cgctattttc tgctccacca gcatcaccac
    15301 gccttgcagt agatcaaggg gttcgaaacc agcgaccacc agcggacgat gaaaatcgct
    15361 ggcgataaaa ttataggcat cagtgccgat aaccatacta acgtgaccgg gcgcaagaaa
    15421 cgcgtcgata ccgttatccg gctcttcaag taaactgcgc agtgttggga tgagcgtaat
    15481 gtgctggcag aagaagtaaa aattctgcac atcgcgggct tttgcctgtt gcaacgtgat
    15541 ggcggtggtc ggcatggtgg tttcaaaacc taagccgaag aacaccactt tgcgggttgg
    15601 attctcctgc gccagtttta acgcatccat tggcgagtaa acgatgcgca catcggcacc
    15661 gcgtgctttt gcctgcaaca gcgacccctg tttccctggt acgcgcatgg catcgccaaa
    15721 ggtacagaag atgacttccg gatggctggc aatctccacg caggtgtcga ttctacccat
    15781 tggcagtacg cacaccgggc aacccggacc gtggataaac tcaacgtttt ccggtagcaa
    15841 ctggtcgagg ccgaatttaa aaatggcgtg ggtatgaccg ccacacactt ccataatccg
    15901 cagagggcgt tcggcggtgt aagagagatg tgaagcacgt tcgcgcagat gctcaattaa
    15961 ctgcattacc tgttccggcg cgcgatattc atcaacaaaa cgcattattt ttcctcgcca
    16021 tacaacagcg cgccgacatc tggctcaacg tcaaacatgt tttgtaaggc gtcgagagtg
    16081 tcgcgtgctt cggcttcatt aattacgctc atggcaaagc caacgtgaac cagtacccac
    16141 tggcccacgc gtggctgacc gttttcatcg cagctgccga ctaacgttaa atcgacatcg
    16201 cgctgaatgc cgcagacgtc gactttcgcc tggttgccgt caatagtgcg gatctggccg
    16261 ggaacgccta tgcacatcgc tgtgtctcca gccagttcag ccactggtcc atcccttcgc
    16321 cgctggtggc ggaaataagg atgatttcaa tttctggatt gacttcacgg gcgcaggcga
    16381 tgcacttctc aacgtcgaaa ttgagatacg gcagcaagtc aactttgttg agcagcatca
    16441 gcgaggcggc ggcaaacatg tgcggatatt tcagcggttt gtcttcacct tcggtaacgg
    16501 aaagcaccgc cactttgtgt ttttcaccga gatcgaagct ggccgggcaa acgaggttac
    16561 cgacgttttc gataaacaga ataccgttat cgtccagtgg caggcgcggc gcggcgtcgg
    16621 caatcatctg tgcgtcaaga tggcagcctt taccggtgtt cacctgaatc gctggggtgc
    16681 cggtagcgcg aatgcgtgcg gcatcgttca cggtttgctg gtcaccttca ataaccgcgc
    16741 acggaacgct gtctttcagg cgcattaaag tttccgtcag cagggtggtt ttaccggaac
    16801 cagggctgga aaccaggttg agcaccagtt gcttgcgggc agcaaagcgc gcgcggttgc
    16861 gttcagccag acggttattt ttgtccagca cgtcaatttc gacttccagc atccgacgct
    16921 ggctcatgcc cggcgcgtga gtgcccgctt cgccatgacc gtaatgcagg tcgccttctt
    16981 cagtctggct gggggtaaat tcaggcgctt taatgccggt gattttcatc ttcgggcgtg
    17041 ccgccggggc aaatggcgcg ctacgaaacg cggaatgagg gttatgttca tcaccctcaa
    17101 tatagaggtt gccttcaccg caaccgcatg ttgtacacat cgctcactcc tggtctattt
    17161 ctatccgccg aatctgtaaa ccgtcgtctg ccacaatctg tagcatgtca ccatgacaca
    17221 gtggacagcg gcggacgcgc tgggtcagta gcgtcacata ctgttggcat gtttcacacc
    17281 agcattcggc ctcttgttct tcgaggtgca gtttacaacc ttccgccacg ctgccgcggc
    17341 aaaccagatc aaaacaaaag gcaagagagc tggtttcgac acaagaaaat gcgccaattt
    17401 tgagccagac cccagttacg cgttttgcgc cgtgttttgc ggcctgctgt tcgatcaatt
    17461 ccagtgcccg ttggcagagg gttatttcgt gcatatcgcc tcccattaac tattgccagc
    17521 tacaagcaat aattgtgcca gtgttgatta tccctgcggt gaataatgtc gatgatgtcg
    17581 aaatgacacg tcgacacggc gacgaaattc atctttagct tagaaatctc tttaataaca
    17641 atgaattaaa agttggcaca gaaaatgctt aaagctggca tctctgttaa acgggtaacc
    17701 tgacaatgac tatttgggaa ataagcgaga aagccgatta catcgcacag cggcatcgtc
    17761 gcctacagga ccagtggcac atctactgca attcgctggt tcaggggatc acgttatcga
    17821 aagcgcgcct gcatcacgcc atgagctgcg cgccggacaa agagctctgt ttcgtccttt
    17881 ttgaacattt tcgcatttac gtcaccctgg cggatggctt taacagccac accatcgagt
    17941 attacgtcga aacaaaagat ggcgaagaca aacagcggat tgcgcaggcg caactgagca
    18001 ttgacggcat gattgatggc aaggtcaaca tccgcgatcg cgaacaggtt ctggaacact
    18061 atctcgaaaa aatcgctggc gtttacgaca gcttatatac cgccattgaa aacaatgtgc
    18121 cggtgaattt aagccaactg gtaaagggac aaagcccggc agcatgagct gaggctttgc
    18181 ccgttttgca ggcgttacgc ctgtttgggg atgggcgtgt cgatgagtgt cgaaaatgac
    18241 atttcatcgg catgttttcg tcaaaaatga caatcacctg aggaatgcct ggtgaatcgt
    18301 tttgtaattg ctgactccac gctctgtatc ggctgccaca cttgtgaggc tgcctgttca
    18361 gagacgcatc gccagcacgg cctgcaatca atgccgcgcc tgagagtgat gctaaatgaa
    18421 aaagaatctg cgccgcagct ctgtcaccac tgtgaagatg caccctgcgc aacggtctgc
    18481 ccggttaacg ccatcactcg tgttgatggt gccgtgcagc tgaatgaaag cttgtgcgta
    18541 agctgcaaac tgtgtggcat cgcctgcccg tttggcgcaa ttgaattttc cggcagccgt
    18601 ccgctggata ttccggcaaa cgccaatacc ccgaaagcgc caccggcacc gcctgctccg
    18661 gcgcgtgtca gcacattgct tgactgggtg ccaggtattc gcgcgatcgc cgtcaaatgt
    18721 gacctttgta gctttgatga acaaggtccg gcctgcgtgc ggatgtgccc gactaaagcc
    18781 ctgcatctgg tggataacac cgatatcgcc cgcgtcagca aacgtaagcg tgagctgact
    18841 tttaacacgg actttggcga tctcaccttg tttcagcagg ctcaaagtgg agaggctaaa
    18901 tgagcgcaat ttccctgatc aatagcggcg tggcgtggtt tgtcgccgtc gctgttctgg
    18961 catttctctt ttcttttcaa aaagcgctaa gtggctggat agccggaatt ggcggcgcgg
    19021 tgggtagcct gtatacggca gccgcgggct tcactgtgct gactggcacg gttggcgtga
    19081 gcggtgcgct gtcgctggta agctacgatg tgcaaatctc tccgcttaac gcgatttggc
    19141 tgattacact cggcctgtgc ggtctgtttg tcagcctcta caacattgac tggcatcgcc
    19201 acgcgcaggt gaaatgcaac ggcttgcaga tcaatatgtt gatggctgcc gccgtctgtg
    19261 ccgtcatcgc cagcaacctc ggcatgttcg tggtaatggc cgaaatcatg gccctgtgtg
    19321 cggtgttcct caccagcaac agcaaagagg gcaaactgtg gtttgcgctg gggcgtcttg
    19381 gcactctgct gctggcgatt gcctgctggc tgctgtggca gcgttacggc acgctggatc
    19441 tgcgcctgct ggatatgcgt atgcaacagc tgccgctcgg ttccgatatc tggctgctcg
    19501 gtgtgattgg ctttggcctg ctggccggga ttattccgct gcacggctgg gtgccacagg
    19561 cacatgccaa cgcctctgcg ccagctgccg cgctgttttc cacggtagtc atgaaaattg
    19621 gcctgctggg cattttaact ctgtcactac tgggcggtaa tgcaccgctg tggtggggga
    19681 tcgcgctgct ggtgctcggt atgatcaccg cattcgtcgg cggtctgtat gcgctgatgg
    19741 aacataatat ccaacgtctg ctggcttacc acaccctgga gaatatcggc attatcctgc
    19801 tggggctggg cgctggcgta acgggtatcg cgctcgaaca accggcgctg attgcccttg
    19861 gtctggttgg tggtctgtac catctgctta accatagcct gttcaagagc gtactgttcc
    19921 ttggtgcggg gagcgtctgg ttccgtaccg gtcatcgcga tatcgaaaaa ctcggtggta
    19981 ttggcaagaa aatgccggtt atctccatcg ccatgttagt cgggctgatg gcaatggctg
    20041 cgctgccgcc gctgaacggt tttgccgggg aatgggttat ctatcaatcc ttcttcaaac
    20101 tgagcaatag tggcgcgttt gttgcccgtc ttctcgggcc gctgctcgcc gtggggctgg
    20161 caattaccgg tgcgctggcg gtgatgtgta tggcgaaagt ttatggcgtt actttcctcg
    20221 gcgcgccgcg taccaaagag gccgaaaacg ccacctgtgc gccgctcctg atgagcgtaa
    20281 gcgtagtggc actggcgatt tgctgcgtaa ttggcggtgt tgctgcgccg tggctactgc
    20341 cgatgctctc tgctgctgta cctctgccgc tggagcctgc taacaccacc gtttctcaac
    20401 caatgatcac gttgctgctg attgcctgcc cgctgctgcc attcatcatt atggcgattt
    20461 gcaaaggcga tcgtttgccg tcgcgttccc gcggtgcggc ctgggtgtgc ggctacgatc
    20521 atgaaaaatc aatggtgatt accgctcacg gttttgccat gccggtgaaa caggcgtttg
    20581 cgccggtgct gaaactacgc aaatggctga atccggtgtc tctggtgccg ggctggcagt
    20641 gcgaggggag tgcgttgctg ttccgccgga tggcgctggt tgaactggcg gtgctggtgg
    20701 tgattattgt ttcacgagga gcctgagaat gagtgtttta tatccgttaa ttcaggcgct
    20761 ggtgctattt gccgttgcgc cgctgctctc cggtattacc cgcgtagcgc gcgcccgtct
    20821 gcataaccgt cgtgggccgg gtgtgttgca ggagtatcgc gacattatca aactgctggg
    20881 ccgccagagc gttggcccgg atgcctccgg ctgggtgttc cgcctgacgc cgtatgtgat
    20941 ggtgggcgtc atgctgacta tcgccactgc gctgccggtg gtgaccgtcg gctctccgct
    21001 gccgcaactg ggcgatttga tcaccttact gtatctcttc gccatcgcgc gtttcttctt
    21061 tgccatttct ggtctggata ctggtagccc gtttaccgct atcggcgcga gccgtgaagc
    21121 gatgcttggc gtgttggttg aaccgatgct gctgcttggt ctgtgggtcg ccgcacaggt
    21181 tgccggttcc accaacatca gcaacatcac cgacaccgtt tatcactggc cactgagcca
    21241 gagcatcccg ctggtgctgg cgctttgtgc ctgtgcgttc gccactttta tcgaaatggg
    21301 caaactgccg ttcgacctgg cggaagccga gcaggagttg caggaaggtc cgctctctga
    21361 gtatagcggc agcggctttg gcgtcatgaa atggggcatc agcctgaaac agctggtggt
    21421 gttgcagacg ttcgtggggg tgtttattcc gtggggacaa atggaaacct tcaccgtcgg
    21481 tgggctgctg ctggcgctgg tgattgccat cgtcaaactg gtggttggcg tcctggttat
    21541 cgcgctgttc gaaaacagca tggcccgtct gcgtcttgat attactccgc gcattacctg
    21601 ggccgggttt ggctttgcat ttttagcgtt cgtctccttg ctggcggcgt gattaaagag
    21661 agtttgagca tgtctgaaga aaaattaggt caacattatc tcgccgcgct gaatgaggca
    21721 tttcctggcg tcgtgctgga ccacgcctgg cagaccaaag atcagctaac catcaccgtg
    21781 aaggtgaact acctgccgga agtggtggag tttctctact acaaacaagg gggttggctg
    21841 tcggtgctgt ttggtaacga cgaacgcaaa ctgaatggtc attacgccgt ttattacgtg
    21901 ctgtcgatgg agaagggcac caagtgttgg gtaacggttc gcgtcgaagt tgacgccaac
    21961 aaaccggagt atccttctgt gacgccgcgc gttccggcgg cagtgtgggg cgagcgtgaa
    22021 gtgcgcgata tgtacggttt gattccggtt ggtctgccgg atgaacgccg tctggtgctg
    22081 ccggatgact ggccggatga actttatcct ctgcgtaaag acagcatgga ttatcgtcag
    22141 cgtccggcac cgactaccga tgctgaaacc tacgagttca tcaacgaact gggcgacaag
    22201 aaaaacaacg tcgtgccgat tggtcccctg cacgtcactt ctgacgaacc gggtcacttc
    22261 cgtctgtttg tcgatggtga aaacattatc gacgccgact accgcctgtt ctacgtccat
    22321 cgcggcatgg aaaaactggc ggaaacccgt atgggttaca acgaagtgac cttcctctct
    22381 gaccgtgtgt gcgggatctg cggctttgct cacagcaccg cctataccac gtcggtggaa
    22441 aacgcgatgg gtattcaggt gccagaacgc gcgcagatga tccgcgccat tctgctggag
    22501 gtagaacgcc tgcactcgca tctgctcaat ctcggcctgg cctgtcactt taccggcttc
    22561 gactccggct ttatgcagtt cttccgcgtg cgtgaaacct ccatgaaaat ggcagagatc
    22621 cttaccggtg cacgtaaaac ctacggcctg aacctgatcg gcgggattcg tcgcgatctg
    22681 ctgaaagacg acatgatcca gacccgccaa ctggcacaac agatgcgtcg tgaagtgcag
    22741 gagctggtgg atgtgctgct gagcacaccg aacatggaac aacgcaccgt cggcattggt
    22801 cgtctggacc cggaaatcgc tcgcgacttc agtaacgttg gcccgatggt tcgtgccagc
    22861 ggtcacgccc gtgatacccg tgccgatcac ccgtttgttg gttatggcct gctgccaatg
    22921 gaagtccaca gcgagcaggg ctgcgacgtt atttcccgtc tgaaagtgcg tatcaacgaa
    22981 gtctataccg cgctgaatat gatcgattac ggtctggata acctgccggg cggcccgctg
    23041 atggtggaag gctttaccta cattccgcac cgtttcgcgc tgggctttgc cgaagcgccg
    23101 cgcggcgacg atatccactg gagcatgacc ggcgacaacc agaagctgta ccgctggcgc
    23161 tgccgtgcgg cgacctacgc gaactggccg accctgcgtt atatgctgcg cggcaacacc
    23221 gtttccgatg cgccgctgat tatcggtagc ctggaccctt gctactcctg taccgaccgc
    23281 atgactgtgg tcgatgtacg taagaagaag agcaaagtgg tgccgtacaa agaacttgag
    23341 cgctacagca ttgagcgtaa aaactcgccg ctgaaataag gaatcgccat gtttaccttt
    23401 atcaaaaaag tcatcaaaac cggcacggcg acttcgtctt atccgctgga gccgattgcg
    23461 gttgataaaa acttccgtgg taagccagag cagaacccgc agcagtgcat tggctgtgcg
    23521 gcttgcgtca atgcctgccc gtcaaacgcc ttaacggttg aaaccgacct cgccactggt
    23581 gagctggcgt ggcagtttga ccttgggcgc tgcatcttct gtgggcgctg cgaagaagtc
    23641 tgcccgacag cggcgatcaa actgtcgcaa gagtacgaac tggcggtgtg gaagaaagaa
    23701 gatttcctgc aacagtcccg cttcgcgctg tgcaactgcc gcgtctgcaa tcgtcctttc
    23761 gccgtccaga aagagatcga ctacgccatt gcgctgctta agcacaacgg tgacagccgc
    23821 gcggaaaacc accgcgaaag ctttgagact tgcccggaat gtaagcgcca gaaatgcctg
    23881 gtgccgtccg accgtattga actgactcgc catatgaaag aggccatctg atgagcaatt
    23941 tattaggccc ccgtgacgcc aacggcattc cggtcccgat gacggtggat gaatccatcg
    24001 ccagcatgaa ggcgtcgtta ctgaaaaaaa tcaaacgttc tgcctacgtt tatcgcgtgg
    24061 actgcggcgg ctgtaacggt tgcgaaatcg aaattttcgc cacgctttcg ccgctgttcg
    24121 atgcagaacg cttcggcatt aaagtcgttc cgtcaccgcg tcatgcggat attttactgt
    24181 ttaccggcgc ggtcacccgt gcaatgcgat cccctgcgct gcgtgcgtgg cagtccgcgc
    24241 cggacccgaa aatctgtatc tcttacggtg cctgcggtaa cagcggcggg atcttccacg
    24301 atctctactg cgtgtggggc ggtacggata aaatcgttcc agtggatgtt tatatccctg
    24361 gctgcccgcc aacgcctgcc gccacgctgt acggctttgc gatggcgctc ggcctgctgg
    24421 agcagaaaat ccacgcccgt gggccgggtg agcaggatga acaaccggcg gagattctgc
    24481 atggcgatat ggtgcagccg ctgcgcgtga aagtggatcg cgaagcacgc cgcctggcgg
    24541 gttatcgtta cggtcgccag attgccgatg atttcctgac gcaattaggg cagggcgaag
    24601 aacaggttgc acgctggctg gaagcggaaa acgatccgcg tctgaacgag attgtcagcc
    24661 atctgaatca tgttgttgaa gaggcgcgta tccgatgagt gaaaaggtgg tgttcagtca
    24721 actgagccgt aaattcattg atgagaacga tgctacgccc gccgaggcgc agcaggtggt
    24781 ctattacagc ctggcgattg gtcaccacct tggggttatc gattgcctgg aagcggcgct
    24841 cacctgcccg tgggatgact atctggcatg gattgccact ctggaggcgg gcagcgaagc
    24901 ccgccgcaaa atggaaggcg tgccgaaata tggtgagatc gtcatcgaca ttaaccatgt
    24961 gccgatgctg gccaacgcat tcgataaagc ccgggcagcg caaacttcgc agcagaagga
    25021 atggagtaca atgctgttaa gtatgctgca tgatattcat caggaaaacg ccatctattt
    25081 gatggtgagg agactgcgtg actgacgttt tactctgtgt tggcaatagc atgatgggcg
    25141 atgatggcgc aggcccgcta ctggcggaaa agtgcgccgc cgcgccgaaa ggtaactggg
    25201 tagtgattga cggcggtagc gcaccggaaa acgacattgt cgctatccgc gaactgcgcc
    25261 cgacacggct gcttattgtc gacgccacgg atatggggct aaaccctggc gagatccgca
    25321 tcatcgaccc ggctgacatc gccgagatgt ttatgatgac tacccataac atgccgctga
    25381 actatcttat cgaccagttg aaagaagata ttggcgaagt gatttttctc ggcatccagc
    25441 cggatatcgt cggcttttac tacccgatga cccagccgat taaagatgcg gtagaaaccg
    25501 tttatcaacg actggaaggc tgggaaggga atggcggctt cgcgcagtta gcggtggaag
    25561 aataattctt cattagggaa ttcagggaat tgaacatcag gacagggatg tgcgcttttt
    25621 tattgagctt agtgctgcct gcgcaggcta ccagctttac cgaatatcta ccgatgagtg
    25681 acagcgaata cgcgcaaaag cgggcgctga aacctctctt aaccctgcct tatgatgccg
    25741 agcaaacctg gcattttagg aaagtgagcg tctgacacgt tactacagag agaacaaaga
    25801 atgactgcta atgctgcacg cgctgttaaa gcaacaagag aactggtcaa tgcagtcccc
    25861 tttctgggag gcagtgactc agaagatgat tatcgtgaag cactggaact ggtcgaatat
    25921 ttgattgaag aagatgatac taatccactt atcgactttc tggcgagccg cattgcagaa
    25981 tatgaaaaca ataacgaaaa gtttgcagag ttcgacaaag ccgttgcagc aatgccggtt
    26041 ggcgtggcgt tacttcgtac gctgattgat caacataatc tgacctatgc ggatttgaag
    26101 aacgaaatcg gctctaaatc cctggtcagt cagattttat ctggacaacg atccctgacg
    26161 atatcccaca ttaaggcact ttcagcccga tttggcgtta agccggaatg gtttctttga
    26221 tttttgactg atgccaaacc ccagattgcc cgatgcgcaa cgcttatcgg gccttgtagg
    26281 ccggataagg cgttcacgcc gcatccggca cggttactta ctctaaatct tcaccattac
    26341 tggcaatcac ttttttatac caccagaacg atttcttacg cgtgcgcgtc agcgtgccgt
    26401 tgcctgcgtc gtcacggtct acgtagacaa aaccgtagcg tttgctcatt tcacccgtag
    26461 aggcggaaac taaatcaata cagccccatg tggtgtagcc catcagcgga atgccgtctg
    26521 caatcgcttc gcccattgcg cggatatgtt cgcgtaaata gctgatgcga tagtcgtcgt
    26581 taatttcgcc attggcagca aattcatctt ttgcgcccag gccgttttcc accagaaaca
    26641 gtggcttctg ataacggtcg tacatcatat tcatggtgat acgcagaccg agtggatcaa
    26701 taccccagcc ccagtcgctc acttgcagat acgggttacg cagcgatttc accacgttcg
    26761 ccgcactgca gttgttggcg ttcatctccg ccgaggcgca gcgcgaggcg taatagctga
    26821 aagagacaaa atcgacggtg tttttcagga tctcatcatc gctcggtgct ttgtcgatgg
    26881 ttaccccttt ttcgcggaat acgcgggcag agtaagccgg atacgcgccc cgcgcctgca
    26941 catcgataaa aaacaggttt tcccgatctt tctccagcgc cgcccagata tcttccggct
    27001 tgcagctgta agggtagaag ttaccgcccg ccagcataca acccacctga ttttgcgggt
    27061 taacctcatg ggcgattttg gtggctagcg cactggcaac cagctggtga tgcgcagcct
    27121 gatatttcat ctgatcctga ttttcccctt cttcaaacac cagacccgcg ccggagaacg
    27181 ggctatgcaa cataatgttg atttcattga aggtaagcca gtatttcacc agaccatcaa
    27241 atgcttcaaa gcaggtccgg gcgtagcggc tgaaaagctc taccagcttg cggttacgcc
    27301 aggagccata ttcggtgacc agatgcatcg gcacatcgaa gtggcataac gtgaccagcg
    27361 gttcgatacc gtacttttta cactcttcaa ataccgcgcg gtaaaaagca atgccctgct
    27421 ggtttggcgt cagttcatcg ccctgcggga agaggcggct ccaggcaatt gaggtacgga
    27481 aaaccttgaa tcccatctct gccatcaggg cgatatcttc tttataacga tgataaaaat
    27541 ccgtcgcctc atggctggga taaaactcgt catcgcgcaa ctgaaaacgt ttttccagtc
    27601 ccagtttcac cgccattcga tgctcgccgt gcgggatcat atcgaccgtg gtcagccctt
    27661 tgccgccttc acggtacgcg ccttcagact ggttggcggc aagcgcgccg ccccataaaa
    27721 aaccttgcgg aaatactgac attttcatcc tcaattaaga cttacttctt tggcgacggt
    27781 cggttgtacg ctctgatgct ttcgcgcctc ggcagtcgct tcctcaacag gaatatcctc
    27841 aaagccgagc aacagtgtga ggatgaacga cagcacgacc gccagcgcca tgacggcgaa
    27901 cacccagacg atgctcattg gattcgccgg atcgaagaac tgcacgctgg taaatagccc
    27961 cggcgctgcc attgagtggc tggcaagccc cgccatacca gcaaccgcgc cgcaaataaa
    28021 accgctgata agactggcga taagcggacg tttcaggcgg atcgccacac catataacgc
    28081 cggttcggaa atccccgcca taatggctga tgccgccgct gcgagcgccg tctggcgcag
    28141 ttccgggttt ttcgttttcc atgccaccgc cagtgatgaa ccgcccagcg acaggttagc
    28201 gccgatctct gacggcatga ccatcccttc tttgccggtt tcggcaatgg tctgaatgat
    28261 ggttggcgta aagacgcggt gcatccctgt cattaccagc agaggccaca gcgcgcccat
    28321 aatggcgact gaaagccagc ccagataacc atgaatggtg taaaccagcg ccgaaatggc
    28381 gctaccgatc cagataccaa tcgggccaat cagcaggatt gccagcggtg cggcaatcaa
    28441 cacaatcagc atcggcttga ggaagttttt cgtcaccgct ggcgtaatgc tgttcaccca
    28501 gcgttcgata tatgacaggc accaggtcat gaccagcgcc gggatcactg tgtaggtgta
    28561 tttcaccgcg gtgaccggaa tcagggcaaa ttcaacatgt tcaccctgtg ccgctttcgc
    28621 catcagctca ataaaactcg gatgtaccag cacacccgca atggcaatcg ccagcgacat
    28681 attggtttta aatttgatgg cggcagaagc agcgaccatc agcggcagga agaagaaagc
    28741 accgtcacca atcacattca gaatggttaa ggtcggcgat ccttttgtca gcacgccgct
    28801 catctcgagg atcatcgcca gcagtttgac catcgatccg ccgataatcg ccgggatcag
    28861 cggtgacatg gtgccgatca gcgcatcgag gatccccgca cctatgcgac gtagcgtaag
    28921 tttgggttta cccacgggct gtgcgggctg catatctccc ggcagcaggc tgacgacttc
    28981 ctgaaaggcc agcgaaaccg tattacctat aatcacctga cactggttgt cactacgtac
    29041 cacgccgagc acgccggaga tggttttcaa cgtcggactg tcgataagct ggtcatcttt
    29101 gataacaaag cgcaaacgcg tcatacagtg cgtgaccgcc gagatgttat caacgccgcc
    29161 cagtgccgtt atcaccgagc ttgccagcgc cgcataattt ttggccatcg gattttatcc
    29221 tgttatcagt agggtagatg tagttgttat cagtcgacgt gacggaaaaa tgccgatgct
    29281 gaaacggaat taggaaaccg gtttcacaaa atcatgaata gttgtcattt aataagcaag
    29341 agagttaatt gttcattttt tatatttgtg aaaccggtca cctgaatatt tttcccgctg
    29401 attgcgcaag gacaatcggc aaacggtgca gtacactgcg ttgattgcgt gtaaagggat
    29461 gaaaatgatg acgacgatgc tggaagtggc gaagcgcgcc ggggtttcaa aagcgaccgt
    29521 ttcccgcgtg ctttcaggta atggctacgt cagccaggag actaaagatc gcgtgtttca
    29581 ggcggtagaa gagagcggtt accgtccaaa cttgctggcg cgcaatctgt cggcgaagag
    29641 tactcagacg ctggggctgg tagtgaccaa cacgctttac catggcattt attttagtga
    29701 attactgttt catgccgcgc gaatggcgga agagaaaggg cgacagttgc tattggcaga
    29761 tggtaaacat agcgccgaag aagagcgcca ggcgattcag tatctgctgg atttgcgctg
    29821 cgacgccatc atgatttacc cgcgcttttt aagcgtggat gagattgatg acatcattga
    29881 cgcacacagt cagccgataa tggtgcttaa tcgccgcctg cgcaaaaaca gcagccatag
    29941 cgtctggtgc gatcataaac agaccagctt taacgccgtg gcagagttga taaccgccgg
    30001 acatcaggag attgctttcc ttaccggctc gatggattcc cccaccagca ttgaacgtct
    30061 tgccggatat aaagacgcgc tggcgcagca tggtattgcg ttcaatgaaa aacttatcgc
    30121 taacggtaaa tggacgcccg ccagcggtgc cgaaggggta gaaacgttgc tcgaacgtgg
    30181 ggctaaattt agcgcgttag ttgccagtaa cgacgatatg gcgataggtg cgatcaaagc
    30241 gttacacgag cgcggcgtag cggtgccaga gcaggtgtca gttatcggat tcgatgatat
    30301 cgctattgcc ccctacatcg ttccggcgct ctccagcgta aaaattccgg tgactgagat
    30361 gattcaggaa attattggac ggctgatttt tatgctcgat ggcggggatt tctcaccgcc
    30421 gaaaaccttc agcggaaaac taatccgccg cggctcactc attgctcttt cgcgataaac
    30481 attgtcatca catgtcatcg tcactagtgt cgatgacatc gtttttcctg tagtacagaa
    30541 tttaatttcc ttaaataaca gtaaattaaa aactggcatg atttgtgaat gtatcggcgc
    30601 attaactgtt attgctggag aatttgatga accgtttcat cattgctgac gcgagtaaat
    30661 gtattggttg ccgtacctgt gaagtagcgt gcgtggtttc ccatcaggag aatcaggact
    30721 gtgcatcgct gaccccggaa acttttttac cgcgaatcca tgtcattaaa ggtgtgaaca
    30781 tttccacggc gacagtctgc cgtcagtgtg aagatgcacc gtgcgctaac gtctgcccga
    30841 atggtgctat cagccgtgat aaagggtttg ttcatgtcat gcaggaacgt tgtattggtt
    30901 gcaaaacctg cgttgtggct tgcccgtatg gtgcgatgga agtggtggtg cgtccggtga
    30961 ttcgcaacag cggcgcaggg ctgaatgtac gggctgacaa agccgaagcc aataaatgcg
    31021 acctgtgcaa ccatcgtgaa gacggcccgg cgtgtatggc ggcttgcccg acccatgcgc
    31081 tgatttgtgt cgatcgtaat aaacttgaac aactgagcgc agaaaaacgc cgccgcacgg
    31141 cgctgatgtt ctaaaaatct tacagaactg ttctggtatc aaaggaattc ccggaaatat
    31201 tcgtagaata cgaagcgggc agtatcgccg acctgtgact gccggatgcg acgctgtcgt
    31261 gtccggctgg gcgggttacg caattcatga tggcgggaat aactcaatgg caaaaaacac
    31321 atcttgcggt gtccaactgc gtattcgtgg caaagtgcag ggcgtcggtt ttcgtccgtt
    31381 tgtctggcaa ctggcacagc aattaaatct tcacggcgat gtctgtaatg acggcgatgg
    31441 cgtagaagtc cggctgctgg aagatccgga aacgtttctt gttcaattgc atcagcactg
    31501 cccgccactg gcgcgtattg atagcgtcga gcgtgagccg tttatctggt cacaactgcc
    31561 cactgagttc accatccgcc agagcgcggg tggcgtcatg aatacgcaaa ttgttcccga
    31621 tgccgcgact tgccctgctt gtcttgccga aatgaatacc ccaggcgaac gacgctggcg
    31681 ttatccgttt atcaactgta ctcactgtgg tccgcgtttc accattattc gcgccatgcc
    31741 ttacgaccgc ccgtttaccg tgatggcggc gtttccgctg tgtccggctt gtgacaaaga
    31801 gtactgtgac ccgctcgatc gtcgcttcca cgcccagccg gtggcctgcc cggagtgtgg
    31861 cccccatctt gaatgggtaa gtcatggtga acatgcagaa caagaggcgg cattacaggc
    31921 agctatcgca cagttaaaaa tgggcaacat tgtcgccatc aaagggattg gcggatttca
    31981 tcttgcctgc gatgcacgta acagtaacgc ggtggcgaca cttcgggcgc gtaaacatcg
    32041 cccggcgaaa ccgctggcgg tcatgttgcc agtggctgac ggtttaccag acgctgcgcg
    32101 ccagttgctt accacgcccg ccgcgccgat tgtgctggtg gataaaaaat atgtacctga
    32161 gctttgtgat gatatcgccc ctgaccttaa cgaagtcggg gtaatgttgc ctgcgaaccc
    32221 gctccagcat ttgctgttac aggaactgca atgcccgctg gtgatgacct ccggcaacct
    32281 gagcggtaaa ccaccggcta tcagcaacga acaggcgctg gcggatttgc agggcattgc
    32341 cgacggattc ttgatacata accgcgacat cgtgcagcgg atggatgatt cggtggtgcg
    32401 cgaaagcggc gaaatgctgc gccgttcgcg ggggtatgtg ccggatgcgc tggctttgcc
    32461 tccgggcttt aaaaatgttc cgcctgtgct gtgtctcggc gcggatctga aaaatacctt
    32521 ctgcctggtg cgcggcgaac aagcggtgtt gagtcagcat ctgggcgatt taagtgacga
    32581 tggcatccag atgcagtggc gcgaagcgtt acgcctgatg caaaacatct acgatttcac
    32641 tccgcaatac gttgtgcatg acgcgcatcc gggctatgtc tccagccagt gggcgcgtga
    32701 gatgaatctg ccgacgcaaa cggtgctgca tcatcatgcc cacgcagcgg cgtgtctggc
    32761 agaacacctc tggcctctgg atggcggaga tgtcattgcg ttgacgctcg acggtattgg
    32821 tatgggggag aacggcgctt tgtggggcgg cgagtgcttg cgggtgaact atcgcgaatg
    32881 ccagcacctg ggcggcttgc ccgcagtggc gcttccgggg ggcgatttgg ctgcgaagca
    32941 gccgtggcga aacctgctgg cgcagtgcct gcgctttgtg ccggagtggc agaattactc
    33001 tgaaacggca agtgtgcaac agcaaaactg gagcgtgctg gcgcgggcta ttgagcgtgg
    33061 aattaacgcg ccgctggcat cgtcgtgtgg acgtttgttc gatgccgtag cggcggcgct
    33121 gggctgtgcg ccagccacgt taagttatga aggtgaagcg gcttgtgcgc tggaggcgct
    33181 ggaggcttcg tgccacggcg tgacgcatcc ggtgacgatg ccgctggtgg acaatcaact
    33241 ggatctcgcc actttctggc agcagtggct gagctggcag gctccggtta atcaacgcgc
    33301 gtgggcgttt catgatgcgc tggcgcaggg ttttgccgcg ttgatgcgtg agcaggctac
    33361 gatgcggggt atcactacgc tggtatttag cggcggagtt attcataacc gtttgctgcg
    33421 tgcacgtctg gcgcattatc tcgctgattt cacattgctc tttccacaga gtttacctgc
    33481 gggcgatggc gggttgtctc tggggcaggg ggttattgtt gcggcgcgtt ggttggcagg
    33541 ggaagtccag aacggataaa acgtgctgca tttggacacc a
//
LOCUS       DADSYM010000038        33098 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2415
            SAMN08039579-rid11723173.denovo.038, whole genome shotgun sequence.
ACCESSION   DADSYM010000038 DADSYM010000000
VERSION     DADSYM010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039579
            Sequence Read Archive: SRR6362533
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 33098)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33098)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980162.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 03:39:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,484
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 4,347
            CDSs (with protein)               :: 4,347
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 43 of 85
            Pseudo Genes (incomplete)         :: 37 of 85
            Pseudo Genes (internal stop)      :: 19 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33098
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039579-rid11723173.denovo.038"
                     /strain="LGE2415"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8302 N 95.9628 W"
                     /collection_date="2015-08-24"
     gene            142..906
                     /locus_tag="J1Y83_001502"
     CDS             142..906
                     /locus_tag="J1Y83_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA7629659.1"
                     /translation="MKKHLFTLTLSSVLAIPAVSHAEFKGGFADIGIHYLDWTSRTTE
                     KSSTKSHKDDFGYLELEGGANFSWGEMYGFFDWENFYNDRHDKPGSEQRYTFKNTNRI
                     YLGDTGFNLYLHAYGTYGSANRVNFHDDMFLYGIGYNFTGSGWWFKPFFAKRYTDQTY
                     YTGDNGYVAGWVAGYSFMLGSEKFTLTNWNEYEFDRDATYAAGNGGKEGLNGAVALWW
                     NATSHITTGIQYRYADDKLGEDFYQDAIIYSIKFNF"
     gene            complement(945..1871)
                     /locus_tag="J1Y83_001503"
     CDS             complement(945..1871)
                     /locus_tag="J1Y83_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBA7629660.1"
                     /translation="MNYSLKQLKVFVTVAQEKSFSRAGERIGLSQSAVSHSVKELENH
                     TGVRLLDRTTREVVLTDAGQQLALRLERLLDELNSTLRDTGRMGQQLSGKVRVAASQT
                     ISAHLIPQCIAESHRRYPDIQFVLHDRPQQWVMESIRQGDVDFGIVIDPGPVGDLQCE
                     AILSEPFFLLCHRDSALAMEDYVPWQALQGAKLVLQDYASGSRPLIDAALARNGIQAN
                     IVQEIGHPATLFPMVAAGIGISILPALALPLPEGSPLVVKRITPVVERQLMLVRRKNR
                     SLSTAAQALWDVVRDQGNALMAGREGDPLYQI"
     gene            1961..2959
                     /locus_tag="J1Y83_001504"
     CDS             1961..2959
                     /locus_tag="J1Y83_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bile acid:sodium symporter"
                     /protein_id="HBA7629661.1"
                     /translation="MKIFRILDPFTLTLITVVLLASFFPARGDFVPFFENLTTAAIAL
                     LFFMHGAKLSREAIIAGGGHWRLHLWVMCSTFVLFPILGVLFAWWKPVNVDPMLYSGF
                     LYLCILPATVQSAIAFTSMAGGNVAAAVCSASASSLLGIFLSPLLVGLVMNVHGAGGS
                     LEQVGKIMLQLLLPFVLGHLSRPWIGDWVSRNKKWIAKTDQTSILLVVYTAFSEAVVN
                     GIWHKVGWGSLLFIVVVSCVLLAIVIVVNVFMARRLGFNKADEITIVFCGSKKSLANG
                     IPMANILFPTSVIGMMVLPLMIFHQIQLMVCAVLARRYKRQTEQLQAQQESSADKA"
     gene            complement(2956..3174)
                     /locus_tag="J1Y83_001505"
     CDS             complement(2956..3174)
                     /locus_tag="J1Y83_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA7629662.1"
                     /translation="MEKEQLIEIANTIMPFGKYKGRRLIDLPEEYLLWFARKDEFPAG
                     KLGELMQITLLIKTEGLTQLVQPLKRPL"
     gene            complement(3176..5191)
                     /gene="ligA"
                     /locus_tag="J1Y83_001506"
     CDS             complement(3176..5191)
                     /gene="ligA"
                     /locus_tag="J1Y83_001506"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HBA7629663.1"
                     /translation="MESIEQQLTELRTTLRHHEYLYHVMDAPEIPDAEYDRLIRELRE
                     LETKHPELITPDSPTQRVGAAPLAAFSQIRHEVPMLSLDNVFDEESFLAFNKRVQDRL
                     KSNEKVTWCCELKLDGLAVSILYENGVLVSAATRGDGTTGEDITSNVRTIRAIPLKLH
                     GENIPARLEVRGEVFLPQAGFEKINEDARRTGGKVFANPRNAAAGSLRQLDPRITAKR
                     PLTFFCYGVGVLEGGELPDTHLGRLLQFKKWGLPVSDRVTLCESAEEVLAFYHKVEED
                     RPTLGFDIDGVVIKVNSLAQQEQLGFVARAPRWAVAFKFPAQEQMTFVRDVEFQVGRT
                     GAITPVARLEPVHVAGVLVSNATLHNADEIERLGLRIGDKVVIRRAGDVIPQVVNVVL
                     SERPEDTREVVFPTHCPVCGSDVERVEGEAVARCTGGLICGAQRKESLKHFVSRRAMD
                     VDGMGDKIIDQLVEKEYVYTPADLFKLTAGKLTGLERMGPKSAQNVVNALEKAKETTF
                     ARFLYALGIREVGEATAAGLAAYFGTLEALEAASIEELQKVPDVGIVVASHVHNFFAE
                     ESNRNVISELLAEGVHWPEPIVINAEEIDSPFAGKTVVLTGSLSQMSRDDAKARLVEL
                     GAKVAGSVSKKTDLVIAGEAAGSKLAKAQELGIEVIDETEMLRLLGS"
     gene            complement(5262..6260)
                     /gene="zipA"
                     /locus_tag="J1Y83_001507"
     CDS             complement(5262..6260)
                     /gene="zipA"
                     /locus_tag="J1Y83_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZipA"
                     /protein_id="HBA7629664.1"
                     /translation="MMQDLRLILIIVGAIAIIALLVHGFWTSRKERSSMFRDRPLKRM
                     KSKRDDDSYDEDVEDDEGVGEVRVHRVNHAPANAQEHEAARPSPQHQYQPPYASAQPR
                     QPVQQPPEAQVPPQHAPRPTQPVQQPVQQPAYQPQPEQPLQQPVSPQVASAPQPVHSA
                     PQPAQQAFQPAEPVAAPQPEPVAEPAPVMDKPKRKEAVIIMNVAAHHGSELNGELLLN
                     SIQQAGFIFGDMNIYHRHLSPDGSGPALFSLANMVKPGTFDPEMKDFTTPGVTIFMQV
                     PSYGDELQNFKLMLQSAQHIADEVGGVVLDDQRRMMTPQKLREYQDIIREVKDANA"
     gene            6490..7251
                     /gene="cysZ"
                     /locus_tag="J1Y83_001508"
     CDS             6490..7251
                     /gene="cysZ"
                     /locus_tag="J1Y83_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate transporter CysZ"
                     /protein_id="HBA7629665.1"
                     /translation="MVSSFTSAPRSGFYYFAQGWKLVSQPGIRRFVILPLLVNILLMG
                     GAFWWLFTQLDVWIPTLMSYVPDWLQWLSYLLWPLAVISVLLVFGYFFSTIANWIAAP
                     FNGLLAEQLEARLTGATPPDTGIFGIMKDVPRIMKREWQKFAWYLPRAIVLLILYFIP
                     GIGQTVAPVLWFLFSAWMLAIQYCDYPFDNHKVPFKEMRTALRTRKITNMQFGALTSL
                     FTMIPLLNLFIMPVAVCGATAMWVDCYRDKHAMWR"
     gene            7436..8407
                     /gene="cysK"
                     /locus_tag="J1Y83_001509"
     CDS             7436..8407
                     /gene="cysK"
                     /locus_tag="J1Y83_001509"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000034402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HBA7629666.1"
                     /translation="MSKIFEDNSLTIGHTPLVRLNRIGNGRILAKVESRNPSFSVKCR
                     IGANMIWDAEKRGVLKPGVELVEPTSGNTGIALAYVAAARGYKLTLTMPETMSIERRK
                     LLKALGANLVLTEGAKGMKGAIQKAEEIVASNPEKYLLLQQFSNPANPEIHEKTTGPE
                     IWEDTDGQVDVFIAGVGTGGTLTGVSRYIKGTKGKTDLISVAVEPTDSPVIAQALAGE
                     EIKPGPHKIQGIGAGFIPANLDLKLVDKVIGITNEEAISTARRLMEEEGILAGISSGA
                     AVAAALKLQEDESFTNKNIVVILPSSGERYLSTALFADLFTEKELQQ"
     gene            8791..9048
                     /gene="ptsH"
                     /locus_tag="J1Y83_001510"
     CDS             8791..9048
                     /gene="ptsH"
                     /locus_tag="J1Y83_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003038086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein Hpr"
                     /protein_id="HBA7629667.1"
                     /translation="MFQQEVTITAPNGLHTRPAAQFVKEAKGFTSEITVTSNGKSASA
                     KSLFKLQTLGLTQGTVVTISAEGEDEQKAVEHLVKLMAELE"
     gene            9093..10820
                     /gene="ptsI"
                     /locus_tag="J1Y83_001511"
     CDS             9093..10820
                     /gene="ptsI"
                     /locus_tag="J1Y83_001511"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate-protein phosphotransferase
                     PtsI"
                     /protein_id="HBA7629668.1"
                     /translation="MISGILASPGIAFGKALLLKEDEIVIDRKKISADQVDQEVERFL
                     SGRAKASAQLETIKTKAGETFGEEKEAIFEGHIMLLEDEELEQEIIALIKDKHMTADA
                     AAHEVIEGQASALEELDDEYLKERAADVRDIGKRLLRNILGLKIIDLSAIQDEVILVA
                     ADLTPSETAQLNLKKVLGFITDAGGRTSHTSIMARSLELPAIVGTGSVTSQVKNDDYL
                     ILDAVNNQVYVNPTNEVIDKMRAVQEQVASEKAELAKLKDLPAITLDGHQVEVCANIG
                     TVRDVEGAERNGAEGVGLYRTEFLFMDRDALPTEEEQFAAYKAVAEACGSQAVIVRTM
                     DIGGDKELPYMNFPKEENPFLGWRAIRIAMDRKEILRDQLRAILRASAFGKLRIMFPM
                     IISVEEVRALRKEIEIYKQELRDEGKAFDESIEIGVMVETPAAATIARHLAKEVDFFS
                     IGTNDLTQYTLAVDRGNDMISHLYQPMSPSVLNLIKQVIDASHAEGKWTGMCGELAGD
                     ERATLLLLGMGLDEFSMSAISIPRIKKIIRNTNFEDAKVLAEQALAQPTTDELMTLVN
                     KFIEEKTIC"
     gene            10861..11370
                     /gene="crr"
                     /locus_tag="J1Y83_001512"
     CDS             10861..11370
                     /gene="crr"
                     /locus_tag="J1Y83_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015833547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HBA7629669.1"
                     /translation="MGLFDKLKSLVSDDKKDTGTIEIIAPLSGEIVNIEDVPDVVFAE
                     KIVGDGIAIKPTGNKMVAPVDGTIGKIFETNHAFSIESDSGVELFVHFGIDTVELKGE
                     GFKRIAEEGQRVKVGDTVIEFDLPLLEEKAKSTLTPVVISNMDEIKELIKLSGSVTVG
                     ETPVIRIKK"
     gene            complement(11413..12264)
                     /gene="pdxK"
                     /locus_tag="J1Y83_001513"
                     /gene_synonym="thiJ"
     CDS             complement(11413..12264)
                     /gene="pdxK"
                     /locus_tag="J1Y83_001513"
                     /gene_synonym="thiJ"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine/pyridoxal/pyridoxamine kinase"
                     /protein_id="HBA7629670.1"
                     /translation="MSSLLLFNDKSRALQADIVAVQSQVVYGSVGNSIAVPAIKQNGL
                     NVFAVPTVLLSNTPHYDTFYGGAIPDEWFSGYLRALQERDALRQLRAVTTGYMGTASQ
                     IKILAEWLTALRKDHPDLLIMVDPVIGDIDSGIYVKPDLPEAYRQYLLPLAQGITPNI
                     FELEILTGKDCRDLDSAIAAAKSLLSDTLKWVVITSASGNEENQEMQVVVVSADSVNV
                     ISHSRVKTDLKGTGDLFCAQLISGLLKGKALTDAVHRAGLRVLEVMRYTQQHESDELI
                     LPPQAEA"
     gene            12369..12737
                     /locus_tag="J1Y83_001514"
     CDS             12369..12737
                     /locus_tag="J1Y83_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfeK family protein"
                     /protein_id="HBA7629671.1"
                     /translation="MKKIICLVVTLLMTLPAYAKLTAHEEARINAMLEGLAQKKDLIF
                     VRNGDEHTCDEAVSHLRLKLGNTRNRIDTAEQFIDKVASSSSITGKPYIVKMPGKSDE
                     NAQPFLHALIAQTDKTVPAQ"
     gene            complement(12740..13651)
                     /gene="cysM"
                     /locus_tag="J1Y83_001515"
     CDS             complement(12740..13651)
                     /gene="cysM"
                     /locus_tag="J1Y83_001515"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase B"
                     /protein_id="HBA7629672.1"
                     /translation="MSTLEQTIGNTPLVKLQRMAPDNGSEVWLKLEGNNPAGSVKDRA
                     ALSMIVEAEKRGEIKPGDVLIEATSGNTGIALAMIAALKGYRMKLLMPDNMSQERRAA
                     MRAYGAELILVTKEQGMEGARDLALEMANRGEGKLLDQFNNPDNPYAHYTTTGPEIWQ
                     QTGGRITHFVSSMGTTGTITGVSRFMREQSKPVTIVGLQPEEGSSIPGIRRWPAEYLP
                     GIFNASLVDEVLDIHQRDAENTMRELAVREGIFCGVSSGGAVAGALRVAKANPGAVVV
                     AIICDRGDRYLSTGVFGEEHFSQGAGI"
     gene            complement(13786..14883)
                     /gene="cysA"
                     /locus_tag="J1Y83_001516"
     CDS             complement(13786..14883)
                     /gene="cysA"
                     /locus_tag="J1Y83_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter ATP-binding
                     protein CysA"
                     /protein_id="HBA7629673.1"
                     /translation="MSIEIANIKKSFGRTQVLNDISLDIPSGQMVALLGPSGSGKTTL
                     LRIIAGLEHQTSGHIRFHGTDVSRLHARDRKVGFVFQHYALFRHMTVFDNIAFGLTVL
                     PRRERPNAAAIKAKVTKLLEMVQLAHLADRYPAQLSGGQKQRVALARALAVEPQILLL
                     DEPFGALDAQVRKELRRWLRQLHEELKFTSVFVTHDQEEATEVADRVVVMSQGNIEQA
                     DAPDQVWREPATRFVLEFMGEVNRLQGTIRGGQFHVGAHRWPLGYTPAYQGPVDLFLR
                     PWEVDISRRTSLDSPLPVQVLEASPKGHYTQLVVQPLGWYNEALTVVMHGDDAPQRGE
                     RLFVGLQHARLYNGDERIETRDEELALAQSA"
     gene            complement(14873..15748)
                     /gene="cysW"
                     /locus_tag="J1Y83_001517"
     CDS             complement(14873..15748)
                     /gene="cysW"
                     /locus_tag="J1Y83_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter permease
                     CysW"
                     /protein_id="HBA7629674.1"
                     /translation="MAEVTQLKRYDARPINWGKWFLIGIGMLVSAFILLVPMIYIFVQ
                     AFSKGLMPVLQNLADPDMLHAIWLTVMIALIAVPVNLVFGILLAWLVTRFNFPGRQLL
                     LTLLDIPFAVSPVVAGLVYLLFYGSNGPLGGWLDEHNLQIMFSWPGMVLVTIFVTCPF
                     VVRELVPVMLSQGSQEDEAAILLGASGWQMFRRVTLPNIRWALLYGVVLTNARAIGEF
                     GAVSVVSGSIRGETLSLPLQIELLEQDYNTVGSFTAAALLTLMAIITLFLKSMLQWRL
                     ENQEKRAQQEEHHEH"
     gene            complement(15748..16581)
                     /gene="cysT"
                     /locus_tag="J1Y83_001518"
     CDS             complement(15748..16581)
                     /gene="cysT"
                     /locus_tag="J1Y83_001518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000458418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter permease
                     CysT"
                     /protein_id="HBA7629675.1"
                     /translation="MFAVSSRRVLPGFTLSLGTSLLFVCLILLLPLSALVMQLAQMSW
                     AQYWEVITNPQVVAAYKVTLLSAFVASIFNGVFGLLMAWILTRYRFPGRTLLDALMDL
                     PFALPTAVAGLTLASLFSVNGFYGEWLAKFDIKVTYTWLGIAVAMAFTSIPFVVRTVQ
                     PVLEELGPEYEEAAETLGATRWQSFRKVVLPELSPALVAGVALSFTRSLGEFGAVIFI
                     AGNIAWKTEVTSLMIFVRLQEFDYPAASAIASVILAASLLLLFSINTLQSRFGRRVVG
                     H"
     gene            complement(16581..17597)
                     /locus_tag="J1Y83_001519"
     CDS             complement(16581..17597)
                     /locus_tag="J1Y83_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter substrate-binding
                     protein"
                     /protein_id="HBA7629676.1"
                     /translation="MAVNLLKKNSLVLVASLLLAGHVQATELLNSSYDVSRELFAALN
                     PPFEQQWAKDNGGDKLTIKQSHAGSSKQALAILQGLKADVVTYNQVTDVQILHDKGKL
                     IPADWQSRLPNNSSPFYSTMGFLVRKGNPKNIHDWNDLVRSDVKLIFPNPKTSGNARY
                     TYLAAWGAADKADGGDKAKTEQFMTQFLKNVEVFDTGGRGATTTFAERGLGDVLISFE
                     SEVNNIRKQYEAQGFEVVIPKTNILAEFPVAWVDKNVQANGTEKAAKAYLNWLYSPQA
                     QTIITDYYYRVNNPEVMDKLKDKFPQTELFRVEDKFGSWPEVMKTHFTSGGELDKLLA
                     AGRK"
     gene            complement(17755..18546)
                     /gene="ucpA"
                     /locus_tag="J1Y83_001520"
     CDS             complement(17755..18546)
                     /gene="ucpA"
                     /locus_tag="J1Y83_001520"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase UcpA"
                     /protein_id="HBA7629677.1"
                     /translation="MGKLTGKTALITGALQGIGEGIARTFARHGANLILLDISPEIEK
                     LADELCGRGHRCTAVVADVRDPASVAAAIKRAKEKEGRIDILVNNAGVCRLGSFLDMS
                     DEDRDFHIDINIKGVWNVTKAVLPEMIARKDGRIVMMSSVTGDMVADPGETAYALTKA
                     AIVGLTKSLAVEYAQSGIRVNAICPGYVRTPMAESIARQSNPEDPESVLTEMAKAIPM
                     RRLADPLEVGELAAFLASDESSYLTGTQNVIDGGSTLPETVSVGI"
     gene            18826..19722
                     /gene="murQ"
                     /locus_tag="J1Y83_001521"
     CDS             18826..19722
                     /gene="murQ"
                     /locus_tag="J1Y83_001521"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HBA7629678.1"
                     /translation="MQLEKMITEGSNAASAEIDRVSTLEMCRIINDEDKTVPLAVERV
                     LPDIAAAIDVIHTQVSGGGRLIYLGAGTSGRLGILDASECPPTYGVKPGLVVGLIAGG
                     EYAIQHAVEGAEDSREGGVNDLKNIGLTAQDVVVGIAASGRTPYVIAGLEYARQLGCR
                     TVGISCNPGSAVSSTAEFAITPVVGAEVVTGSSRMKAGTAQKLVLNMLSTGLMIKSGK
                     VFGNLMVDVVATNEKLHVRQVNIVKNATGCNAEQAEAALIACERNCKTAIVMVLKNLD
                     ADEAKKCLDQHGGFIRKALEKE"
     gene            19726..21150
                     /gene="murP"
                     /locus_tag="J1Y83_001522"
     CDS             19726..21150
                     /gene="murP"
                     /locus_tag="J1Y83_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylmuramic acid transporter subunit
                     IIBC"
                     /protein_id="HBA7629679.1"
                     /translation="MAKEISSELLNTILTRVGGPGNIASCGNCMTRLRLGVHDSSLVD
                     PDIKTLEGVKGVILTSDQVQVVFGPGKAHRAAKAMSELLGEAPVQDAAEIAAQNKRQL
                     KARQTSGVQQFLAKFATIFTPLIPGFIAAGLLLGIATLIATVMHVPADAQGTLPDALN
                     FMKVFSKGLFTFLVILVGYNAAQAFGGTGVNGAIIAALFLLGYNPTATTGYYAGFHDF
                     FGLPIDPRGNIIGVLIAAWACARIECMVRRFMPDDLDMLLTSLITLLITATLAYLIIM
                     PLGGWLFEGMSWLFMHLNSNPLGCAVLAGLFLIAVVFGVHQGFIPVYLALMDSQGFNS
                     LFPILSMAGAGQVGAALALYWRAQPHSALRSQVRGAIIPGLLGVGEPLIYGVTLPRMK
                     PFVTACLGGAAGGLFIGLIAWWGLPMGLNSAFGPSGLVALPLMTSAQGILPAMAVYAG
                     GILVAWVSGFIFTTLFGCRNVNLD"
     gene            complement(21328..22227)
                     /gene="yfeX"
                     /locus_tag="J1Y83_001523"
     CDS             complement(21328..22227)
                     /gene="yfeX"
                     /locus_tag="J1Y83_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311329.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porphyrinogen peroxidase"
                     /protein_id="HBA7629680.1"
                     /translation="MSQVQSGILPEHCRAAIWIEANVKGEVDALRAASKTFADKLATF
                     EAKFPDAHLGAVVAFGNNTWRALSGGVGAEELKDFPGYGKGLAPTTQFDVLIHILSLR
                     HDVNFSVAQAAMEAFGDCIEVKEEIHGFRWVEERDLSGFVDGTENPAGEETRREVAVI
                     KDGVDAGGSYVFVQRWEHNLKQLNRMSVHDQEMMIGRTKEANEEIDGDERPETSHLTR
                     VDLKEDGKGLKIVRQSLPYGTASGTHGLYFCAYCARLHNIEQQLLSMFGDTDGKRDAM
                     LRFTKPVTGGYYFAPSLDKLMAL"
     gene            complement(22323..22898)
                     /locus_tag="J1Y83_001524"
     CDS             complement(22323..22898)
                     /locus_tag="J1Y83_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RpoE-regulated lipoprotein"
                     /protein_id="HBA7629681.1"
                     /translation="MKSLRLMLCAMPLMLTGCSTMSSVNWSAANPWNWFGSSTKVSEQ
                     GVGELTASTPLQEQAIADALDGDYRLRSGMKTANGNVVRFFEVMKGDNVAMVINGDQG
                     TISRIDVLDSDIPADTGVKIGTPFSDLYSKAFGNCQKADSDDNRAVECKAEGSQHISY
                     QFSGEWSGPEGLMPSDDTLKNWKVSKIIWRR"
     gene            complement(22959..23408)
                     /locus_tag="J1Y83_001525"
     CDS             complement(22959..23408)
                     /locus_tag="J1Y83_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2919 domain-containing protein"
                     /protein_id="HBA7629682.1"
                     /translation="MKSTEFHPVHYDAHGRLRLPLLFWLVLLLQARTWVLFVIAGASR
                     EQGTALLNLFYPDHDNFWLGLIPGIPAVLAFLLSGRRASFPRIWHVLYFLLLLAQVVL
                     LCWQPWLWLNGESVSGIGLALVVADIVALIWLLTNRRLRACFNEEKE"
     gene            complement(23395..23820)
                     /locus_tag="J1Y83_001526"
     CDS             complement(23395..23820)
                     /locus_tag="J1Y83_001526"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family acetyltransferase"
                     /protein_id="HBA7629683.1"
                     /translation="MDIRVFRQEDFEEVITLWERCDLLRPWNDPEMDIERKMNHDISL
                     FLVAEVNGEVVGTVMGGYDGHRGSAYYLGVHPEFRGRGIANALLNRLEKKLIARGCPK
                     IQINVPEDNDMVLGMYERLGYEHADVLSLGKRLIEDEEY"
     gene            24034..24903
                     /gene="amiA"
                     /locus_tag="J1Y83_001527"
     CDS             24034..24903
                     /gene="amiA"
                     /locus_tag="J1Y83_001527"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiA"
                     /protein_id="HBA7629684.1"
                     /translation="MSTFKPLKTLTSRRQVLKAGLAALTLSGMSQAIAKEEPLKTSNG
                     HSKPKAKKSGGRRVVVLDPGHGGIDTGAIGRNGSKEKHVVLAIAKNVRSILRNHGIDA
                     RLTRSGDTFIPLYDRVEIAHKHGADLFMSIHADGFTNPKAAGASVFALSNRGASSAMA
                     KYLSERENRADEVAGKKATDKDHLLQQVLFDLVQTDTIKNSLTLGSHILKKIKPVHKL
                     HSRNTEQAAFVVLKSPSVPSVLVETSFITNPEEERLLGTAAFRQKIATAIAEGVISYF
                     HWFDNQKAHSRKR"
     gene            24907..25806
                     /gene="hemF"
                     /locus_tag="J1Y83_001528"
     CDS             24907..25806
                     /gene="hemF"
                     /locus_tag="J1Y83_001528"
                     /EC_number="1.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-dependent coproporphyrinogen oxidase"
                     /protein_id="HBA7629685.1"
                     /translation="MKPDAHQVKQFLLNLQDTICQQLSAVDGAEFVEDSWQREAGGGG
                     RSRVLRNGGVFEQAGVNFSHVHGEAMPASATAHRPELAGRSFEAMGVSLVVHPHNPYV
                     PTSHANVRFFIAEKPGAEPVWWFGGGFDLTPFYGFEEDAIHWHRTARDLCLPFGEDVY
                     PRYKKWCDEYFYLKHRNEQRGIGGLFFDDLNTPDFDHCFAFMQAVGKGYTDAYLPIVE
                     RRKTMAYGERERNFQLYRRGRYVEFNLVWDRGTLFGLQTGGRTESILMSMPPLVRWEY
                     DYQPEDGSPEAALSEFIKVRDWV"
     gene            complement(25812..26864)
                     /gene="eutR"
                     /locus_tag="J1Y83_001529"
     CDS             complement(25812..26864)
                     /gene="eutR"
                     /locus_tag="J1Y83_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator EutR"
                     /protein_id="HBA7629686.1"
                     /translation="MKKTRTANLHHLYHEPLPENLKLTPKVEVDNVHQRQTTDVYEHA
                     LTITAWQQIYDQLHPGKFHGEFTEILLDDIQVFREYTGLALRQSCLVWPNSFWFGIPA
                     TRGEQGFIGSQCLGSAEIATRPGGTEFELSTPDDYTILGVVLSEDVITRQANFLHNPD
                     RVLHMLRSQSALEVKEQHKAALWGFVQQALATFCENPENLHQPAVRKVLGDNLLMAMG
                     AMLEEAQPMVTAESISHQSYRRLLSRAREYVLENMSEPVTVLDLCNQLHVSRRTLQNA
                     FHAILGIGPNAWLKRIRLNAVRRELISPWSQSMTVKDAAMQWGFWHLGQFATDYQQLF
                     AEKPSLTLHQRMREWG"
     gene            complement(26910..27410)
                     /gene="eutK"
                     /locus_tag="J1Y83_001530"
     CDS             complement(26910..27410)
                     /gene="eutK"
                     /locus_tag="J1Y83_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311336.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutK"
                     /protein_id="HBA7629687.1"
                     /translation="MINALGLLEVDGMVAAIDAADAMLKAANVRLLSHEVLDPGRLTL
                     VVEGDLAACRAALDAGCAAAMRTGRVISRKEIGRPDDDTQWLVTGFNRQPKQPVKEPD
                     APVIVAESADELLALLTSVRQGMTAGEVAAHFGWPLEKARNALEQLFSAGTLRKRSSR
                     YRLKPH"
     gene            complement(27423..28082)
                     /gene="eutL"
                     /locus_tag="J1Y83_001531"
     CDS             complement(27423..28082)
                     /gene="eutL"
                     /locus_tag="J1Y83_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutL"
                     /protein_id="HBA7629688.1"
                     /translation="MPALDLIRPSVTAMRVIASVNAEFARELKLPPHIRSLGLISADS
                     DDVTYIAADEATKQAMVEVVYGRSLYAGAAHGPSPTAGEVLIMLGGPNPAEVRAGLDA
                     MVANIENGAAFQWANDAENTAFLAHVVSRTGSYLSSTAGITLGDPMAYLVAPPLEATY
                     GIDAALKSADVQLVTYVPPPSETNYSAAFLTGSQAACKAACNAFTDAVLEIARNPIQR
                     A"
     gene            complement(28092..28979)
                     /locus_tag="J1Y83_001532"
     CDS             complement(28092..28979)
                     /locus_tag="J1Y83_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase subunit EutC"
                     /protein_id="HBA7629689.1"
                     /translation="MDQKQIEEIVRSVMASMGQTAPAPSEAKCATTNCAAPVTSESCA
                     LDLGSAEAKAWIGVENPHRADVLTELRRSTVARVCTGRAGPRPRTQALLRFLADHSRS
                     KDTVLKEVPEEWVKAQGLLEVRSEISDKNLYLTRPDMGRRLCAEAVEALKAQCVANPD
                     VQVVISDGLSTDAITVNYEEILPPLMAGLKQAGLKVGTPFFVRYGRVKIEDQIGEILG
                     AKVVILLVGERPGLGQSESLSCYAVYSPRMATTVEADRTCISNIHQGGTPPVEAAAVI
                     VDLAKRMLEQKASGINMTR"
     gene            complement(29000..30361)
                     /locus_tag="J1Y83_001533"
     CDS             complement(29000..30361)
                     /locus_tag="J1Y83_001533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase subunit EutB"
                     /protein_id="HBA7629690.1"
                     /translation="MKLKTTLFGNVYQFKDVKEVLAKANELRSGDVLAGVAAASSQER
                     VAAKQVLSEMTVADIRNNPVIAYEDDCVTRLIQDDVNETAYNQIKNWSISELREYVLS
                     DEISVDDIAFTRKGLTSEVVAAVAKICSNADLIYGAKKMPVIKKANTTIGIPGTFSAR
                     LQPNDTRDDVQSIAAQIYEGLSFGVGDAVIGVNPVTDDVENLSRVLDTIYGVIDKFNI
                     PTQGCVLAHVTTQIEAIRRGAPGGLIFQSICGSEKGLKEFGVELAMLDEARAVGAEFN
                     RIAGENCLYFETGQGSALSAGANFGADQVTMEARNYGLARHYDPFIVNTVVGFIGPEY
                     LYNDRQIIRAGLEDHFMGKLSGISMGCDCCYTNHADADQNLNENLMILLATAGCNYIM
                     GMPLGDDIMLNYQTTAFHDTATVRQLLNLRPSPEFERWLESMGIMANGRLTKRAGDPS
                     LFF"
     gene            complement(30373..31776)
                     /gene="eutA"
                     /locus_tag="J1Y83_001534"
     CDS             complement(30373..31776)
                     /gene="eutA"
                     /locus_tag="J1Y83_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase reactivating factor
                     EutA"
                     /protein_id="HBA7629691.1"
                     /translation="MNTRQLLSVGIDIGTTTTQVIFSHLELVNRAAVSQVPRYEFIKR
                     EISWQSPVFFTPVDKQGGLKEAELKTLILEQYQAAGIAPESVDSGAIIITGESAKTRN
                     ARPAVMALSRSLGDFVVASAGPHLESVIAGHGAGAQTLSEQRLCRVLNIDIGGGTANY
                     ALFDAGKISGTACLNVGGRLLETDSQGRVVYAHKPGQMIVDECFGPGTDARSLTGGQL
                     VQVTRRMAELIVEVIDGTLSPLAQALMQTDLLPAGVTPEIITLSGGVGECYRHQPADP
                     FCFADIGPLLATALHDHPRLRELNVQFPAQTVRATVIGAGAHTLSLSGSTIWLEGVQL
                     PLRNLPVAIPIDETDLVSAWQQALIQLDLCPKTDAYVLALPASLPVRYAAVLTVINAL
                     VDFVARFPNPHPLLVVAGQDFGKALGMLLRPQLQQLPLAVIDEVIVRAGDYIDIGTPL
                     FGGSVVPVTVKSLAFPS"
     gene            complement(31773..32999)
                     /gene="eutH"
                     /locus_tag="J1Y83_001535"
     CDS             complement(31773..32999)
                     /gene="eutH"
                     /locus_tag="J1Y83_001535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization protein EutH"
                     /protein_id="HBA7629692.1"
                     /translation="MGINEIIMYIMMFFMLIAAVDRILSQFGGSARFLGKFGKSIEGS
                     GGQFEEGFMAMGALGLAMVGMTALAPVLAHVLGPVIIPVYEILGANPSMFAGTLLACD
                     MGGFFLAKELAGGDVAAWLYSGLILGSMMGPTIVFSIPVALGIIEPSDRRYLALGVLA
                     GIVTIPIGCIAGGLVAMYSGVQINGQPVEFTFALILMNMIPVLIVAVLVALGLKFIPE
                     KMINGFQIFAKFLVALITLGLAAAVVKFLLGWELIPGLDPIFMAPGDKPGEVMRAIEV
                     IGSISCVLLGAYPMVLLLTRWFEKPLMSVGKVLNMNNIAAAGMVATLANNIPMFGMMK
                     QMDTRGKVINCAFAVSAAFALGDHLGFAAANMNAMIFPMIVGKLIGGVTAIGVAMMLV
                     PKEDATAAKTEAEAQS"
BASE COUNT         7788 a         9098 c         8415 g         7797 t
ORIGIN      
        1 atatatttat attttttcac tattaatagg ctgaaaaaac tgtattggta ctctcctttc
       61 ccttgcatta acaatatgcc gcttttaatc aaccttttat taagcaatca actaataacg
      121 atcggcatag ggaattatgc tatgaaaaaa catcttttta ctctgacact ctcttctgta
      181 ttggcgatac cagcagtatc ccatgcagaa tttaaaggcg gttttgctga tataggtatt
      241 cattatcttg actggactag cagaaccact gagaaatcgt cgaccaaatc acacaaagat
      301 gattttggat atctggaact ggaaggcggc gctaacttta gttggggaga aatgtacggg
      361 ttcttcgact gggaaaactt ttataatgat cgtcatgata aaccgggtag cgagcagcgt
      421 tatacattta aaaataccaa ccgtatttac ctgggtgaca ccggatttaa tctctacttg
      481 catgcgtatg gcacctacgg ttctgcgaat cgtgtgaatt tccatgacga tatgttcctt
      541 tacgggatcg gctacaattt caccggcagc ggttggtggt tcaaaccgtt ctttgcaaaa
      601 cgctatacag accaaaccta ttataccggc gataacggtt atgtcgccgg ttgggttgca
      661 ggttacagct ttatgctggg cagtgagaaa ttcaccctca ccaactggaa cgagtacgag
      721 tttgatcgtg atgccaccta cgcggcaggt aatggcggca aagaaggtct gaacggtgcc
      781 gtcgcacttt ggtggaatgc aacttcacac atcaccacag ggattcagta tcgttatgcg
      841 gatgacaaac tgggcgaaga tttttaccag gatgcgataa tctactccat caaatttaat
      901 ttctgattga ttactgccgg agctcccggc agtatgaatt cactttatat ctgatacaac
      961 ggatcccctt cccgcccagc cattagcgca ttgccctggt ctcgtaccac gtcccataac
     1021 gcttgtgcgg cggtggaaag tgaacggttc ttcctgcgca ccagcatcaa ctggcgttca
     1081 accactggcg taatgcgctt taccaccagt gggctacctt caggcaatgg tagcgccagt
     1141 gcgggaagaa tactgatgcc gatgcctgcg gctaccatcg ggaacagcgt cgccggatga
     1201 ccaatctcct gcacaatatt cgcctgaata ccattgcgcg ccagcgctgc gtcaatcagc
     1261 ggtcggctgc ctgacgcgta atcctgcaac accaatttcg ctccctgtaa tgcctgccag
     1321 gggacataat cttccatggc caaagcgcta tcgcgatggc aaagcagaaa gaaaggctcg
     1381 gaaagaatcg cttcacattg cagatcgccc actgggcctg gatcgatgac gatgccaaaa
     1441 tcgacatctc cctggcgaat actttccatc acccactgct gcggacgatc gtgcaggaca
     1501 aactgaatat ctggatagcg gcgatggctt tcagcaatgc attgcggaat aagatgcgcg
     1561 gaaatggtct ggctggcagc gacccgaact tttccgctca gttgttgccc catacgcccg
     1621 gtatcgcgca acgtgctgtt aagttcatcc agcagtcgct caagacgcaa agccaactgc
     1681 tgccctgcat ctgtaagcac cacttcacgc gtggttctgt ccagcaggcg aacaccagta
     1741 tgattttcca gctccttcac actgtgactc actgccgact ggctcaggcc gatacgctct
     1801 cctgcacgac taaaactttt ctcctgcgct actgtgacga aaacctttaa ttgctttaaa
     1861 gaataattca tctgttttct tcatgaatgg atgcaataaa tcaattttat ttctcaaact
     1921 ggaagaagca caatagaacc atcgatcatc tggagtcttt atgaaaattt ttcgtatcct
     1981 cgatcctttc accttaaccc tgatcacggt ggtgttgctg gcctctttct ttccggccag
     2041 aggcgatttc gtccccttct ttgaaaatct gaccactgca gcgattgctc tgctgttctt
     2101 tatgcacggc gcgaagttgt cgcgtgaggc gattattgct ggcggtggtc actggcgact
     2161 gcatttgtgg gtaatgtgca gcaccttcgt gctgtttccg attctgggcg tgctgtttgc
     2221 ctggtggaaa ccggtaaatg tcgacccgat gctctactcc ggttttctct acttgtgcat
     2281 tctccccgcg accgtgcagt ctgcaatcgc cttcacgtca atggcgggcg gtaacgtcgc
     2341 ggcggcggtt tgttctgcgt cagcatccag cctgctgggg attttccttt caccattgct
     2401 ggttggtctg gtgatgaatg ttcacggtgc agggggcagc cttgagcagg tcggtaaaat
     2461 tatgctgcaa ctgttgctgc catttgtgtt ggggcatctt tcccggcctt ggattggtga
     2521 ctgggtgtcg cgtaataaaa aatggattgc gaaaactgac cagacgtcca ttctgttggt
     2581 ggtttatacg gcattcagcg aagccgtcgt taacggtatc tggcacaaag ttggctgggg
     2641 atcattgctg tttatcgtgg tggtcagttg cgttcttctg gctatcgtga ttgtagttaa
     2701 cgtctttatg gcacgccgac tgggcttcaa taaggcagat gaaattacta tcgtcttttg
     2761 tggttcgaaa aagagtctgg caaatggcat cccgatggca aacattctgt tccccacatc
     2821 ggtgatcggt atgatggtgc tgcccctgat gattttccat cagatccaat tgatggtctg
     2881 tgcggtgctg gcgcgtcgat acaaacgcca gaccgaacag ttacaggcgc agcaggaaag
     2941 cagcgccgat aaagcttaaa gcggacgctt caggggctgg accagttgcg tcagcccctc
     3001 ggttttaatc agcagcgtga tttgcattag ctcaccgagc ttccctgccg ggaactcatc
     3061 tttgcgggca aaccacagca gatactcctc cggcaggtcg attaagcgac gccctttgta
     3121 cttgccaaac ggcattatcg tattggctat ttcaatcagc tgctcttttt ccatctcagc
     3181 tacccagcaa acgcagcatt tccgtttcgt cgatgacttc aatgcccagt tcctgcgcct
     3241 tcgccagttt agatcctgca gcttcacccg ctatcaccag atcggttttc ttcgacacgc
     3301 tgcccgcgac tttcgccccc agttcgacca gtcgagcttt agcgtcatca cgcgacatct
     3361 ggcttaagct gcccgtaagc accacggttt taccagcaaa cggactgtca atctcttccg
     3421 cattgataac gatcggctca ggccagtgaa caccttccgc caacagctcg ctgatgacat
     3481 tgcggttgct ttcttcggca aagaagttgt gaacgtggga ggcaacgaca attccaacat
     3541 caggcacctt ttgcagctct tcaatcgacg cggcttccag cgcttccagc gtgccgaaat
     3601 atgccgccag acctgctgcg gtagcctcgc cgacttcacg gataccaagt gcatagagaa
     3661 agcgagcaaa ggtggtttct ttcgcttttt ccagcgcgtt aaccacgttt tgtgccgatt
     3721 tcggccccat gcgctccagt ccggtcagtt tgcctgcggt gagtttgaac agatccgccg
     3781 gagtgtagac atattctttt tcaaccagtt gatcgatgat tttgtcgccc attccgtcaa
     3841 catccatcgc acggcgggaa acaaagtgtt tcagcgactc tttacgctgc gcaccgcaaa
     3901 tcaggccacc ggtacagcgg gcaaccgctt caccttccac acgctcgacg tcagaaccac
     3961 ataccggaca atgcgtcggg aatacaacct cacgggtatc ttccgggcgt tcagaaagca
     4021 cgacgttaac cacctgcggg atcacatcgc ctgcgcggcg aatcaccact ttatcgccaa
     4081 tgcgtaaacc aagacgttcg atttcgtcag cattatgcag tgtagcgtta ctcaccaaca
     4141 cgccagcaac atggacaggt tccagacgcg caacaggcgt aatcgcgcca gtacgcccaa
     4201 cctgaaactc gacgtcacgc acaaaggtca tctgttcctg cgccgggaat ttaaacgcta
     4261 ccgcccagcg cggggcacgc gcgacaaagc caagctgctc ctgctgtgcc agtgagttga
     4321 ccttaatcac cacgccgtcg atatcaaagc ccagcgtcgg acggtcttct tctactttgt
     4381 ggtagaacgc cagtacttct tccgccgatt cacaaagcgt tacccgatcg ctgaccggca
     4441 aaccccactt tttaaattgc agtaaacggc caagatgagt atccggcagc tcgccacctt
     4501 ccagaacacc gacgccatag cagaagaaag tgagcggtcg cttcgctgta atacgcggat
     4561 caagctgacg cagtgaacca gctgccgcat tacgtgggtt agcaaacact ttcccgcccg
     4621 tgcgtcgcgc atcttcgtta atcttttcga accccgcctg cggcaggaac acttcaccac
     4681 gcacttccag acgcgccggg atattctcac cgtgtagctt cagcggaatg gcgcgaatag
     4741 tacgcacatt agacgtgata tcttccccgg tggtgccatc gccacgagtc gcggcactga
     4801 ctaaaacgcc attttcatac agaatactga cggcaagacc atccagcttc agctcacagc
     4861 accaggtgac tttctcgttg cttttcagac ggtcctgcac acgtttgttg aaagcaagaa
     4921 agctttcttc atcaaaaacg ttatccagcg acagcattgg cacttcatgg cggatctggc
     4981 tgaaagccgc cagcggcgct gcacctacac gttgggtagg cgaatcaggc gtaatcagtt
     5041 ctggatgttt ggtttccagc tcgcgcagtt cgcgtatcag cctgtcgtat tcagcgtcgg
     5101 gaatttccgg cgcatccatc acatgataaa gatattcatg atggcgaagc gtcgttcgca
     5161 gttctgtcag ttgttgttcg attgattcca tatcgcacca tcaatgctaa aaacccccga
     5221 caagcggggg ttcgaagagg agttaatttg ccttaagtgt atcaggcgtt ggcgtctttg
     5281 acttcgcgga tgatgtcctg gtactcgcgc aatttctgcg gagtcatcat acggcgctga
     5341 tcgtcaagca ctacaccgcc cacttcatcg gcaatatgct gcgcagattg cagcatcagc
     5401 ttaaagttct gcagctcgtc accgtaagac ggtacctgca taaagatagt gacacccgga
     5461 gtagtgaaat ccttcatttc aggatcaaag gttcccggtt tcaccatatt cgccaggctg
     5521 aataacgccg ggccgctgcc atccgggcta agatgacgat ggtaaatgtt catatcgcca
     5581 aaaatgaagc ccgcttgttg aatgctgtta agaagcagtt caccgtttaa ctcgctaccg
     5641 tgatgcgccg cgacgttcat gataatcacc gcttctttgc gcttcggttt atccataact
     5701 ggagccggtt ccgctacagg ctcaggctgt ggtgccgcta cgggttctgc aggctgaaaa
     5761 gcctgttgtg ccggttgcgg tgctgaatgc actggctgcg gcgctgacgc gacctgtggc
     5821 gaaactggct gctgcaacgg ctgttcaggc tgcggctgat aggcaggctg ctgcacgggt
     5881 tgctgcaccg gctgcgttgg acgcggagca tgttgcggcg gtacctgcgc ttcaggcggc
     5941 tgctggaccg gttgacgcgg ctgcgcagac gcataaggcg gttggtactg gtgttgcggc
     6001 gacggacgag cagcctcatg ctcctgggcg ttagccgggg catgattcac gcggtgaaca
     6061 cgaacctcac caacgccctc atcatcttcg acatcctcgt cataagaatc gtcgtcacgt
     6121 tttgacttca ttcgttttaa tggccgatcg cggaacatag aagatcgttc tttacggctg
     6181 gtccagaaac catgtaccag taaagcgatt atggcgatcg cgccaacaat gattaatatc
     6241 agacgcaaat cctgcatcat tatattctct gttgttctaa caccttgcca ccacggcaaa
     6301 catttactca ctaagagtat ttgccgatta cctcaagtgc aagtgcacta ttaactttca
     6361 cagcacaaag atagatgaaa tcgtgctttt tgctgttttt tcgaacatat cctaactgtc
     6421 cattgcgcaa ttacccggta aaatacgcag aattttcctg ggattggtca aaaggagctc
     6481 atcctgatta tggtttcatc attcacatct gccccacgca gcggttttta ctattttgcg
     6541 caaggctgga agctcgtctc gcaacctggg attcggcgtt tcgttatttt accgctgctg
     6601 gtcaatattt tgttgatggg gggcgcattc tggtggctct ttacgcaact cgatgtctgg
     6661 atcccgactc tcatgagtta tgttccggac tggttacaat ggctgagcta tctgttgtgg
     6721 cctctggctg tcatctctgt gctgttagtg tttggctatt tcttctcaac gattgctaac
     6781 tggattgccg ctccgttcaa tggtttattg gctgaacaac tggaagcacg attgactggc
     6841 gctacaccgc cagataccgg gattttcggt atcatgaaag atgtgccgcg aatcatgaaa
     6901 cgcgaatggc aaaaatttgc ctggtatctg ccgcgcgcaa ttgtattgct aattctttac
     6961 ttcatccctg gtattgggca aaccgtcgcg ccggtactgt ggttcctgtt tagcgcctgg
     7021 atgttagcca tccagtattg cgattacccc ttcgataacc acaaagtgcc gtttaaagag
     7081 atgcgcaccg ccctgcgcac acgcaaaatc accaatatgc agtttggtgc tttaaccagc
     7141 ctgtttacga tgatcccgtt gcttaatctg ttcattatgc ccgttgccgt ttgtggcgcg
     7201 acggcgatgt gggtcgattg ctatcgcgat aaacacgcca tgtggcggta acaatctacc
     7261 ggttattttg taaaccgttt gtgtgaaaca ggggtggctt atgccgcccc ttattccatc
     7321 ttgcatgtca ttatttccct tctgcatata gatatgctaa atccttactt ccgcatattc
     7381 tctgagcggg tatgctacct gttgtatccc aatttcatac agttaaggac aggccatgag
     7441 taagattttt gaagataact cgctgactat cggtcacacg ccgctggttc gcctgaatcg
     7501 catcggtaac ggacgcattc tggcgaaggt ggaatctcgt aaccccagct tcagcgttaa
     7561 gtgccgtatc ggtgccaaca tgatttggga tgccgaaaag cgcggcgtgc tgaaaccagg
     7621 cgttgaactg gttgaaccaa ccagcggtaa taccgggatt gcactggcct atgtagctgc
     7681 cgctcgcggt tacaaactta ccctgaccat gccagaaacc atgagtattg aacgccgcaa
     7741 gctgctgaaa gcgttaggtg caaacctggt gctgaccgaa ggtgctaaag gcatgaaagg
     7801 cgcaatccaa aaagcagaag aaattgtcgc cagcaatcca gagaaatacc tgctgctgca
     7861 acaattcagc aatccggcaa accctgaaat tcacgaaaaa accaccggtc cggagatctg
     7921 ggaagatacc gacggtcagg ttgatgtatt tattgctggc gttgggactg gcggtacgct
     7981 gactggcgtc agccgctaca ttaaaggcac caaaggcaag accgatctta tctctgtcgc
     8041 cgttgagcca accgattctc cagttatcgc ccaggcgctg gcaggtgaag agattaaacc
     8101 tggtccgcat aaaattcagg gtattggcgc tggttttatc ccggctaacc ttgatctcaa
     8161 gctggtcgac aaagtcattg gcatcaccaa tgaagaagcc atttctaccg cacgtcgtct
     8221 gatggaagaa gaaggtattc ttgctggtat ctcttctgga gcagctgttg ccgcagcgtt
     8281 gaaactacaa gaagatgaaa gctttaccaa taagaatatt gtggttattc tgccgtcatc
     8341 gggtgagcgt tatttaagca ctgcattgtt tgccgatctt ttcactgaga aagaactgca
     8401 acagtaatgc cagcttgtaa aaaatgcgta aaaaagcacc tttttaggtg cttttttgtg
     8461 gcctgcttca aactttcgcc cctcctggca ttgattcagc ctgtcggaac tggtatttaa
     8521 ccagactaat tattttgatg cgcgaaatta atcgttacag gaaaagccaa agctgaatcg
     8581 attttatgat ttggttcaat tcttccttta gcggcataat gtttaatgac gtacgaaacg
     8641 tcagcggtca acacccgcca gcaatggact gtattacgct cttcgtgcgt cgcgtctgtt
     8701 aaaaactggc gctaacaata caggctaaag tcgaaccgcc aggctagact ttagttccac
     8761 aacactaaac ctataagttg gggaaataca atgttccagc aagaagttac cattaccgct
     8821 ccgaacggtc tgcacacccg ccctgctgcc cagtttgtaa aagaagctaa gggcttcact
     8881 tctgaaatta ctgtgacttc caacggcaaa agcgccagcg cgaaaagcct gtttaaactg
     8941 cagactctgg gcctgactca aggtaccgtt gtgactatct ccgcagaagg cgaagacgag
     9001 cagaaagcgg ttgaacatct ggttaaactg atggcggaac tcgagtaatt tcccgggttc
     9061 ttttaaaaat cagtcacaag taaggtaggg ttatgatttc aggcatttta gcatccccgg
     9121 gtatcgcttt cggtaaagct ctgcttctga aagaagacga aattgtcatt gaccggaaaa
     9181 aaatttctgc cgaccaggtt gatcaggaag ttgaacgttt tctgagcggt cgtgccaagg
     9241 catcagccca gctggaaacg atcaaaacga aagctggtga aacgttcggt gaagaaaaag
     9301 aagccatctt tgaagggcat attatgctgc tcgaagatga ggagctggag caggaaatca
     9361 tagccctgat taaagataag cacatgacag ctgacgcagc tgctcatgaa gttatcgaag
     9421 gtcaggcttc tgccctggaa gagctggatg atgaatacct gaaagaacgt gcggctgacg
     9481 tacgtgatat cggtaagcgc ctgctgcgca acatcctggg cctgaagatt atcgacctga
     9541 gcgccattca ggatgaagtc attctggttg ccgctgacct gacgccgtcc gaaaccgcac
     9601 agctgaacct gaagaaggtg ctgggtttca tcaccgacgc gggtggccgt acttcccaca
     9661 cctctatcat ggcgcgttct ctggaactgc ctgctatcgt gggtaccggt agcgtcacct
     9721 ctcaggtgaa aaatgacgac tatctgattc tggatgccgt aaataatcag gtttacgtca
     9781 atccaaccaa cgaagttatt gataaaatgc gcgctgttca ggagcaagtg gcttctgaaa
     9841 aagcagagct tgctaaactg aaagatctgc cagctattac gctggacggt caccaggtag
     9901 aagtgtgcgc taacattggt acggttcgtg acgttgaagg tgcagagcgt aacggcgctg
     9961 aaggcgttgg tctgtatcgt actgagttcc tgttcatgga ccgcgacgcg ctgcccactg
    10021 aagaagaaca gtttgctgct tacaaagcag tggctgaagc gtgtggctct caggcggtta
    10081 tcgttcgtac catggacatc ggcggcgaca aagagctgcc atacatgaac ttcccgaaag
    10141 aagagaaccc gttcctcggc tggcgcgcta tccgtatcgc gatggatcgt aaagagatcc
    10201 tgcgcgatca gctccgcgct atcctgcgtg cctctgcttt cggtaaattg cgcatcatgt
    10261 tcccgatgat catctctgtt gaagaagtgc gtgcactgcg caaagagatc gaaatctaca
    10321 aacaggaact gcgcgacgaa ggtaaagcgt ttgacgagtc aattgaaatc ggcgtaatgg
    10381 tggaaacacc ggctgccgca acaattgcac gtcatttagc caaagaagtt gatttcttta
    10441 gtatcggcac caatgattta acgcagtaca ctctggcagt tgaccgtggt aatgatatga
    10501 tttcacacct ttaccagcca atgtcaccgt ccgtgctgaa cttgatcaag caagttattg
    10561 atgcttctca tgctgaaggc aaatggactg gcatgtgtgg tgagcttgct ggcgatgaac
    10621 gtgctacact tctgttgctg gggatgggtc tggacgaatt ctctatgagc gccatttcta
    10681 tcccgcgcat taagaagatt atccgtaaca cgaacttcga agatgcgaag gtgttagcag
    10741 agcaggctct tgctcaaccg acaacggacg agttaatgac gctggttaac aagttcattg
    10801 aagaaaaaac aatctgctaa tccacgagat gcggcccaat ttactgctta ggagaagatc
    10861 atgggtttgt tcgataaact gaaatctctg gtttccgacg acaagaagga taccggaact
    10921 attgagatca ttgctccgct ctctggcgag atcgtcaata tcgaagacgt gccggatgtc
    10981 gtttttgcgg aaaaaatcgt tggtgatggc attgctatca aaccaacggg taacaaaatg
    11041 gtcgcgcctg tagacggcac cattggtaaa atctttgaaa ccaaccacgc gttctctatc
    11101 gaatctgata gcggcgttga gctgttcgtc cacttcggta tcgacaccgt tgaactgaaa
    11161 ggcgaaggct tcaagcgtat tgctgaagaa ggtcagcgcg tgaaagttgg tgatactgtc
    11221 attgaatttg atctgccgct gctggaagag aaagccaagt caaccctgac tccggttgtt
    11281 atctccaaca tggacgaaat caaagaactg atcaaactgt ccggtagcgt aaccgtgggt
    11341 gaaaccccgg ttatccgcat caagaagtaa ttcttgccgc agtgaaaaat ggcgcccatc
    11401 ggcgccattt ttttatgctt cagcctgagg cggcaaaatc aattcatcgc tctcatgctg
    11461 ctgggtatag cgcatcactt ccagtacgcg caaccccgcg cggtgtaccg catcggttaa
    11521 cgccttccct ttcagcaaac cactgataag ctgagcacaa aacaggtcgc cagtcccttt
    11581 caggtcagtt ttaacccggg aatgggaaat gacattcacg ctgtcggcac tgaccaccac
    11641 cacctgcatc tcctgatttt cttcattgcc ggaggcgctg gtaatcacca cccactttaa
    11701 tgtgtctgaa agcagacttt ttgcggcagc aatggcactg tcgagatcgc ggcaatcttt
    11761 accggtcagg atttccagct caaagatatt gggggtaatt ccctgtgcca gcggcagtaa
    11821 atattgtcga tacgcctcgg gaaggtcagg tttgacataa attccgctat caatatcgcc
    11881 aatcaccgga tcgaccatga tcaataggtc aggatggtct ttgcgtagcg cagtcagcca
    11941 ctcggcaagg attttgattt gcgatgccgt tcccatatag cccgtggtta cagcacgaag
    12001 ttggcgcagc gcatcacgct cctgaagcgc acgcaaataa ccgctaaacc attcatccgg
    12061 aatcgcacca ccgtagaaag tgtcataatg cggcgtattg ctcagcaata ccgtcggcac
    12121 ggcaaagaca ttcaggccgt tctgtttgat agcaggcacc gcaatgctgt tgcctacgct
    12181 gccgtacact acctgcgact gcacggcaac gatatccgcc tgcagtgccc tgctcttatc
    12241 gttaaacaac aacaaactac tcatttaatt ttttctcctt gccgatgatc ctcatcgtat
    12301 tccagccgaa actttacctg attctggcag tcaaatcggc tattacaaaa caaggataag
    12361 gtaattcagt gaagaaaatc atttgtctgg tcgttacgct gctaatgaca ctccccgctt
    12421 atgcgaagtt aacggcccat gaagaagccc gtatcaacgc catgctggag ggattagcac
    12481 agaaaaagga tttgatattt gtgcgcaacg gtgatgaaca tacctgcgat gaagcggttt
    12541 ctcatctgcg cctgaagctg ggcaataccc gtaatcgcat tgatacagcc gagcagttta
    12601 ttgacaaggt ggcctcgtcg tcatcgatta ccgggaagcc gtatatcgtg aagatgcccg
    12661 gtaagagcga tgagaacgca cagccttttt tacatgcgtt aattgcgcag acggataaaa
    12721 cggtgcctgc gcaataatct taaatccccg ccccctggct aaaatgctct tccccaaaca
    12781 ccccggtaga aaggtagcga tcgccacgat cgcagatgat cgccaccacc accgcgccag
    12841 ggttagcttt tgccacccgc agtgctccgg caaccgcgcc gccggagctt acgccgcaga
    12901 aaatcccttc ccgcactgcc agttcgcgca tggtattttc cgcatcgcgc tgatgaatat
    12961 ccagcacctc atccaccaga gaagcattga aaatccccgg cagatattcc gcaggccagc
    13021 ggcgaatgcc gggaatactg ctgccttctt ccggctgcag gccaacaatg gtcaccggtt
    13081 tggattgttc gcgcataaag cgtgacacgc cggtgatagt gccggttgtt cccatgctgg
    13141 agacaaaatg ggtaatacgc ccgccagttt gctgccagat ttccggcccg gtagtggtgt
    13201 aatgggcgta agggttatcg ggattattga actgatcgag caactttcct tcgccgcgat
    13261 tcgccatctc cagcgccaga tcgcgtgcgc cttccatgcc ctgttctttg gtgaccagaa
    13321 tcagttctgc accgtaggca cgcatcgccg cacggcgttc ctggctcatg ttgtcgggca
    13381 tcagcaattt catgcgatag cctttaagcg ccgcaatcat tgccagcgca atgccggtgt
    13441 taccactggt ggcttcgatc agcacatcac ccggtttaat ctcgccgcgc ttttccgcct
    13501 cgacaatcat cgaaagcgct gcacggtctt ttaccgatcc cgccgggtta ttgccttcca
    13561 gttttaacca cacttcactg ccgttatccg gcgccattcg ctgcaacttc accagaggcg
    13621 tattgcctat tgtttgttct aatgtactca cgatctctgt ccatacgtgg tgttgcctga
    13681 tgcgacgctt acgcgtctta tcagacctac aggttacaaa accttgccat taaaaagccc
    13741 ggatcgcggg aagcgcctcc gggcgtttaa cattcactca acctatcagg cgctttgtgc
    13801 gagagcaagt tcctcatcgc gggtttcaat acgctcgtcg ccgttataca gccgcgcatg
    13861 ttgcagacca acgaataaac gctcgccacg ttgcggggcg tcgtcgccgt gcatcacgac
    13921 cgtcagcgct tcgttgtacc accccagcgg ctgcacgact aattgggtgt agtggccttt
    13981 cgggctggct tccagtacct gtaccggcag cggcgaatcg aggctggtac ggcggctgat
    14041 atccacttcc cacggacgca ggaagagatc caccggcccc tgatacgcag gtgtatagcc
    14101 cagcggccag cgatgtgcgc caacatggaa ctgcccgccg cgaatggttc cctgcaggcg
    14161 gttcacttcg cccataaatt cgagtacaaa acgggtcgct ggttcgcgcc atacctggtc
    14221 cggtgcatca gcctgctcga tattgccctg gctcatcacc actacacgat cggctacttc
    14281 ggtcgcttct tcctggtcat gggtcacaaa cacgctggtg aatttcagct cttcatgtaa
    14341 ctgacgcagc cagcgacgca actctttacg cacctgcgca tccagcgcgc caaacggttc
    14401 atcaagcagc agaatttgcg gttctacggc aagcgcgcgc gccagcgcca cacgctgttt
    14461 ctggccgccg gaaagctgcg caggatagcg atctgccaga tgggcaagct gcaccatctc
    14521 cagcaatttt gtcactttcg ctttgatcgc cgccgcattc gggcgctcgc gacgcggtag
    14581 caccgtcagg ccaaaagcga tattgtcaaa taccgtcata tggcggaaca acgcgtaatg
    14641 ctggaacacg aaaccgactt tacggtcgcg cgcatgcagg cggctaacgt cggtgccgtg
    14701 gaagcgaata tgcccgctgg tttgatgctc cagcccggcg ataatccgca gcagcgtggt
    14761 tttcccggaa ccggacggcc ccagcaatgc gaccatctga ccagaaggaa tatccagtga
    14821 gatatcgttc agcacctggg tgcgaccaaa agacttctta atattggcaa tctcaatgct
    14881 catgatgttc ctcctgctgc gcgcgttttt cctgattctc cagacgccac tgcaacatac
    14941 tctttaaaaa cagggtgata atcgccatca gcgtcaacaa cgcggcagcg gtaaaggagc
    15001 cgacggtgtt gtagtcctgc tccagcaatt caatctgtaa cggcagcgaa agtgtttcgc
    15061 cgcgaatcga gccggaaact accgacaccg caccaaactc gccaatcgcg cgggcgttgg
    15121 tcagcaccac gccataaagc agcgcccaac ggatgttcgg cagcgtgacg cgacggaaca
    15181 tctgccagcc agatgcgccc agcaaaatcg ctgcttcgtc ttcctggctg ccctggctta
    15241 acattaccgg caccagctcg cgcaccacaa acggacaggt cacgaagatg gtgaccagca
    15301 ccattcccgg ccaggagaac ataatttgca gattatgttc atccagccag ccaccgagcg
    15361 ggccgttaga gccgtaaaac agcagataca ccagaccggc aaccaccggc gacacggcaa
    15421 acggaatgtc cagcagcgtc agcagcaact ggcgtccggg gaagttaaag cgcgtcacca
    15481 gccaagccag cagaatgccg aacaccaggt tcaccggcac ggcaatcagc gcaatcatca
    15541 ccgtcagcca gatggcgtgc agcatgtccg gatcggccag attctgtaaa accggcatca
    15601 gccctttgct gaacgcctgc acgaagatgt aaatcatcgg caccagcagg atgaacgccg
    15661 aaaccagcat cccgatgcca atcagaaacc atttgcccca gttaatcggg cgcgcgtcat
    15721 aacgcttcaa ttgtgtcact tccgccatta atgacctacc acacgccgac caaagcgact
    15781 ttgcagagtg ttaattgaga acagcaacag cagcgatgcc gcgaggatca ccgaggcaat
    15841 cgcgctcgcc gccgggtaat caaactcctg taagcgaaca aagatcatca gcgacgttac
    15901 ttctgttttc caggcgatgt ttccggcgat aaaaatcacc gcgccaaact caccgaggct
    15961 gcgggtaaat gacagcgcca cacccgccac cagcgccgga gatagttccg gcagcaccac
    16021 tttgcgaaaa ctctgccagc gtgttgcgcc gagcgtttct gccgcctctt catattccgg
    16081 gcctaactct tccagcaccg gctgcacggt gcgcaccaca aacggaatgc tggtaaaggc
    16141 catcgccacc gcaatcccca gccaggtata ggtgactttg atatcaaact tcgccagcca
    16201 ttcaccgtaa aaaccgttca cggaaaagag tgaggccagc gttaaaccgg ccactgccgt
    16261 tggcagcgca aagggtaaat ccatcagcgc atcaagcaac gtgcggccag ggaagcgata
    16321 acgggttaag atccacgcca tcaacaggcc gaaaacgccg ttaaaaatcg acgccacaaa
    16381 cgccgacagc agcgtcactt tataggccgc gaccacctgc gggttggtga tcacctccca
    16441 gtactgcgcc cagctcatct gggccagttg catcaccagc gcggagagcg gcagcagcaa
    16501 aatcaggcac acaaacagca gactggtgcc gaggcttaag gtaaagcccg gcagcacgcg
    16561 tcttgaagaa acggcaaaca ttacttgcgc cccgccgcta acagcttgtc taactcgccg
    16621 ccgctggtga agtgggtttt catcacttcc ggccaggagc caaatttgtc ttccacgcgg
    16681 aacagctcgg tctgcgggaa tttatctttc agtttgtcca tgacttccgg gttatttacg
    16741 cggtagtaat agtcggtgat gatggtttgc gcctgcgggc tgtagagcca gttcaggtag
    16801 gcttttgccg ctttttccgt gccgttggcc tgcacatttt tatcgaccca cgccaccggg
    16861 aattccgcca gaatgttggt tttcggaatc accacttcaa agccctgcgc ttcatactgt
    16921 ttacggatgt tgttcacttc cgactcgaag ctgatcagca catcgcccag gccgcgctcg
    16981 gcaaaagtgg tggtcgcgcc acgaccgcca gtatcgaaca cttcaacgtt tttcaggaac
    17041 tgggtcataa actgttcggt tttggcttta tcaccaccgt cggctttgtc cgctgcgccc
    17101 catgccgcaa ggtaggtata acgcgcgtta cccgacgttt tcgggttcgg gaaaatcagc
    17161 ttcacgtcag agcgtaccag gtcgttccag tcgtggatat tcttcgggtt gcccttacgc
    17221 accaggaagc ccatggtgga gtagaacggc gagctattat tcggcaggcg cgactgccag
    17281 tccgctggga tcagcttgcc tttatcgtgc aggatttgta cgtcggtcac ctggttataa
    17341 gtgacaacgt cggcttttaa accctgcaaa attgccagcg cctgttttga tgatccggca
    17401 tgagattgtt taatcgtcag tttgtcgcca ccgttatctt ttgcccattg ttgctcaaac
    17461 ggcgggttca gggcggcaaa cagctcgcgg gagacgtcat aagaactgtt cagcagttcc
    17521 gttgcctgta catggcccgc cagtagcaga gaagcaacca gcacgagtga gttctttttc
    17581 agtaagttaa cggccattgc gcacccttat aaatttaatg actttctaat agccatcata
    17641 tttataacgg acgtgaaagg agtaacggtt ttatataccg tttggtgatt tggaagttga
    17701 aaagggaata agacgaaatc ctccccacaa atgcagggag gaaacagagg tgaatcagat
    17761 gccgacgcta accgtctccg gcagtgtgct gccgccatca atcacattct gtgtaccggt
    17821 taaatagctg gattcatccg atgcgaggaa ggccgccagt tcgccgactt ccagcggatc
    17881 ggcgaggcga cgcatcggga ttgctttcgc catttcagtc agcaccgatt ctggatcttc
    17941 cgggttcgac tggcgggcaa tgctttccgc cattggcgtg cgaacgtatc cagggcagat
    18001 ggcgttaacg cgaataccag actgcgcgta ctccaccgcc agcgatttag tcaggccaac
    18061 aatcgccgct ttcgttaagg cgtacgccgt ttcgcccggg tctgccacca tatcgccagt
    18121 gactgaagac atcatcacaa tccggccatc tttgcgggca atcatctccg gcagcaccgc
    18181 cttcgtgacg ttccatacgc cttttatatt gatatcaata tggaaatcgc gatcttcatc
    18241 gctcatatca aggaaactgc ccagacgaca aacgcctgcg ttattcacca gaatatcaat
    18301 gcgcccttct ttttccttag cgcgcttgat agctgcggct accgacgccg ggtcacgcac
    18361 atcggcgaca accgccgtac agcgatgacc acgaccacac agttcgtccg ccagcttttc
    18421 gatctcaggg gagatatcca gcaagattag gtttgcgcca tgacgcgcaa aagttctggc
    18481 aattccttcg ccaattccct gcaatgcgcc cgtaatcagt gctgtcttgc ccgtgagttt
    18541 acccatttta atgctctcct tgttaataca gagcgttaca ctccattccc tctgaaaata
    18601 caggataaac aaaatattgc tgcggatcag ctcactaatt caggaaaatc tcaatttagt
    18661 cgctgcttta tgcagcacct caagcgttac gtcagcgcgc aatcattttc agtgacatca
    18721 catgagtaat attcggactt aatgattcct ttgtgtctat caaatcggga tatatgatcc
    18781 catttatctg caattatttg atcgcaaata ttaaggtcac caccgatgca acttgaaaag
    18841 atgattaccg aaggctcgaa cgccgcctcg gctgaaattg accgcgtatc aacgctggaa
    18901 atgtgccgga ttatcaacga tgaagataaa accgtaccac tcgccgttga gcgcgtactg
    18961 ccggatatcg ccgcggcgat cgatgttatc cacacccagg taagcggcgg cggccgtctg
    19021 atttatctcg gtgcgggaac atccggtcgt ctggggattc tggatgccag cgaatgtccg
    19081 ccgacctacg gcgtgaaacc gggcctggta gttggtttga ttgctggcgg cgaatatgcc
    19141 attcagcacg cggtggaagg cgcagaagat agccgggaag gtggtgttaa tgatctgaaa
    19201 aatatcggct taaccgcgca ggatgtggtg gttggtattg cggccagcgg acgcacgccg
    19261 tatgtgattg ccgggctgga atacgcgcgc caactcggct gccgtacagt gggcatttcc
    19321 tgtaatccgg ggagtgccgt ttcatccacc gcagagtttg ctattacgcc ggtagttggt
    19381 gccgaagttg ttaccggttc ttcgcgaatg aaagcaggca cggcgcagaa actggtgctc
    19441 aacatgctct ccaccgggct gatgattaaa tccggcaaag tgttcggcaa cctgatggtc
    19501 gatgtggtcg ccaccaacga aaaactgcat gtgcgccagg tcaatatcgt caaaaacgct
    19561 accggatgta atgccgaaca agcggaagcg gcgttaattg cttgcgagcg caactgtaaa
    19621 acggccattg tgatggtact gaaaaatctc gatgccgatg aagctaaaaa atgcctggat
    19681 caacacggcg gttttattcg caaggctctg gaaaaggaat aacccatggc taaagagatc
    19741 agcagtgaac ttctgaacac cattcttacc cgggtcggcg gaccgggaaa tatcgccagt
    19801 tgtggtaact gtatgacgcg cctgcgtctg ggtgtacatg acagttcatt ggttgatccc
    19861 gatatcaaaa cgctggaagg cgtgaagggc gtcattttga ccagcgatca ggtgcaggtg
    19921 gtttttgggc caggcaaagc gcatcgtgcc gcaaaagcca tgagcgagct gctgggcgag
    19981 gcaccggtac aggatgccgc agaaatcgcc gcccagaata aacgccagtt aaaagccaga
    20041 caaacttccg gcgtgcaaca atttctcgcc aaattcgcca ccatcttcac gccactgatc
    20101 cccggtttta ttgccgccgg tctgctgctg gggatagcga cgttaattgc cacggtgatg
    20161 cacgttccgg cagatgctca gggcacgcta cccgatgcgc tgaattttat gaaggtgttc
    20221 agcaaaggcc tgttcacctt cctggtaatt ctggtgggct ataacgcggc ccaggcattt
    20281 ggtggcacgg gcgtaaatgg cgcaattatc gctgcgctct ttttgcttgg ttataaccca
    20341 actgcaacca cgggttacta cgccggattt cacgatttct ttggtctgcc catcgatccg
    20401 cgcggcaata ttatcggcgt gttgattgcc gcatgggcct gcgcccgcat cgaatgcatg
    20461 gtgcgccgct ttatgccgga cgacctcgac atgctgttga cctcattaat caccctgctg
    20521 atcaccgcta cgcttgcgta cctgatcatt atgccgctgg gcggctggct gtttgaaggt
    20581 atgtcgtggc tgtttatgca cctgaacagt aatccgctcg ggtgtgcggt tttagccggg
    20641 ctgttcctga tcgccgtggt atttggtgtg catcagggct ttattcctgt ttatctggcg
    20701 ttaatggaca gccagggatt caacagctta tttcccatcc tttcaatggc aggcgcaggc
    20761 caggtgggcg cggcgctggc gctctactgg cgggcgcaac cgcacagtgc attacgcagt
    20821 caggtccgcg gggcgattat tcccggtctg cttggcgttg gcgaaccgct gatttacggt
    20881 gtcaccctgc cccgcatgaa accgtttgtt accgcctgtt taggcggcgc ggcgggcggt
    20941 ttgtttatcg ggctgatagc ctggtggggt ctgccgatgg gcttaaacag cgcctttggg
    21001 ccgtctggtc tggtggcgct gccgctgatg acttccgcgc aaggcatcct tccggcaatg
    21061 gcggtttacg ctggcggtat tctggtggca tgggttagtg ggtttatttt caccacactc
    21121 tttggctgcc gtaacgtcaa tctggactga ttatgaaacg gacaatgctc tatctttctc
    21181 tgctggctat tagctgtagc gtcagcgcca caaaataccc tgttctgaca gaaagtagtt
    21241 gatcaagtgt atgcggcgtt aaagcagtaa taaaaaggcc agtcagttgt gttactgcct
    21301 ggcctttaat caatgaatca gaaacgctta cagcgccatc aacttgtcca gcgacggcgc
    21361 gaagtaatag ccgccagtga ccggtttggt gaaacgcagc atcgcatcac gcttaccatc
    21421 ggtatcgcca aacatgctca gcagttgctg ctcaatgtta tgcagacgcg cgcagtaggc
    21481 gcagaagtac agaccgtgag tgccactggc ggtgccgtac ggcaggctct gacgaacaat
    21541 cttcagccct ttgccatctt ctttcagatc aacgcgggtc aggtgagagg tttccggacg
    21601 ttcgtcgccg tcaatctctt cgttggcctc tttggtgcgc ccgatcatca tctcttgatc
    21661 gtgaacgctc atccgattga gctgtttcag gttgtgctcc caacgctgca caaacacata
    21721 gctgccgccc gcatccacgc cgtctttgat aacagccact tcgcggcgcg tctcttcacc
    21781 cgccgggttt tctgtaccgt caacaaagcc gctcaggtca cgctcttcaa cccaacggaa
    21841 gccgtggatc tcttctttca cttcaatgca gtcaccaaag gcttccatcg ccgcctgagc
    21901 gacagagaag ttcacgtcgt gacgcagaga aagaatgtgg atcaacacat cgaactgggt
    21961 cgtcggtgca agacctttac cgtagcccgg aaaatctttc agctcttcgg ccccaacgcc
    22021 gccgctcaga gcgcgccagg tattgttacc aaaggcaacc accgcaccaa gatgcgcgtc
    22081 cgggaatttc gcttcaaaag ttgccagttt gtcggcaaat gttttactgg ccgcacgcag
    22141 ggcgtcaact tcccctttca cgttggcttc gatccaaatc gccgcgcggc aatgttctgg
    22201 caaaatgcca ctctgaacct gagacattgt tcctcctgaa aataataatg ccacgcaatc
    22261 cgtggcattg ttgggcgcta ttttacgtgt tttttcgcgg cggcggtttg ttcagacgca
    22321 aattaacgcc gccagataat tttactgact ttccagtttt tcagggtatc gtccgaaggc
    22381 attaaccctt cagggccact ccattccccg ctgaactggt agctaatatg ttgactgcct
    22441 tcggctttac attcgacggc acgattatca tcactgtcgg ccttttggca attgccaaat
    22501 gctttgctgt aaaggtcact aaacggtgta ccgattttaa caccggtgtc agcaggaata
    22561 tcgctatcca gcacatcaat gcggctgatc gtgccttgat caccgttaat caccatcgcc
    22621 acgttgtcgc ctttcatcac ttcaaaaaag cgcaccacgt tgccgttcgc ggttttcatt
    22681 ccgctgcgca ggcgataatc gccatcaagc gcatcagcaa tggcttgttc ttgcagtggt
    22741 gtggacgccg ttaattcacc cacgccctgc tcgctcactt tggtggatga tccaaaccag
    22801 ttccacgggt tagcggccga ccagttaact gacgacatcg tggaacagcc ggtcagcatc
    22861 aacggcatag cgcataacat taaacgcagc gatttcatgt cacttccttt ggttattcaa
    22921 taacgttgct tggagtgcaa attcaccaaa aagtgccgtt attctttttc ttcattaaaa
    22981 caagcgcgta aacgtcgatt ggtcagcagc cagattaacg ccacgatatc cgccactacc
    23041 agcgccagac cgataccgct aacggattca ccgttcagcc acagccacgg ttgccaacaa
    23101 agtaaaacca cttgcgccaa cagcaacaga aaatagagca catgccagat acggggaaat
    23161 gaagcccgcc gaccactcag caaaaatgcc agcaccgccg gaataccggg aatcaacccc
    23221 agccagaaat tatcgtgatc gggataaaac agatttagca gcgcagtacc ctgctcgcgc
    23281 gacgctccgg caatgacaaa cagcacccag gttcgcgcct gaagcaatag cacaagccag
    23341 aagagtaagg gtaaacgcag gcgaccgtgc gcatcataat ggacaggatg aaactcagta
    23401 ctcttcatct tcaatcaaac gcttacccag actcagcacg tcggcgtgtt catatcccag
    23461 gcgttcatac attccgagca ccatgtcgtt atcttccggc acgttgatct gaattttcgg
    23521 acagccgcga gcaatcagct ttttctccaa ccgattaagc aacgcattag caatcccacg
    23581 cccacgaaac tcgggatgca cgccaagata ataagcagac ccgcgatgcc cgtcataacc
    23641 gcccatcacc gttccgacca cttcaccgtt cacctcagcg accagaaaca aactgatgtc
    23701 atggttcatc ttacgctcga tgtccatttc cggatcgttc cacggacgca gcaagtcgca
    23761 acgctcccaa agggtgatga cctcttcgaa atcttcctgg cgaaatacgc gtatatccat
    23821 ggtattcgtt acctttttgc gggttaaaag gctgattatg gcgtgaacgg tcgaattagc
    23881 caatatctga cgaaaatcgg ttgaaaaagt ggcataatgg ggagttgtca actattgaaa
    23941 tgaaaagtaa aacaattctc aacagcaaac cgtcgtaacg gattacgcga tacgatataa
    24001 catctggaac tttattatta caactcaggc cgtatgagca cttttaaacc actaaaaaca
    24061 ctcacttcgc gccgccaggt gctgaaagcc ggtttggctg ccctgacgtt gtcaggaatg
    24121 tcgcaagcca tcgccaaaga agaaccgtta aaaaccagta acggacacag caagccgaaa
    24181 gcaaaaaaaa gcggcggcag acgtgtcgtt gttctcgatc caggccacgg cgggattgat
    24241 actggagcga tcggacgcaa cggttcgaaa gaaaaacatg tggtgctggc aattgccaag
    24301 aatgtccgtt ccatattgcg taatcatggc attgatgcgc gtttaacgcg ttctggcgat
    24361 acgtttatcc cactgtacga tcgcgttgaa atcgcccata aacacggcgc agacctgttt
    24421 atgtcaattc acgccgacgg ttttaccaac ccgaaagctg ccggtgcttc ggtatttgcc
    24481 ctctctaacc gcggtgcaag tagcgcaatg gcgaaatacc tgtctgaacg cgaaaaccgc
    24541 gccgatgaag ttgccggtaa aaaagcgact gacaaggatc acctattgca gcaagtgctg
    24601 tttgatctgg tgcaaaccga tactattaaa aacagcctga cgctcggctc gcatattctg
    24661 aaaaagatta agccggtgca taaactgcac agccgcaaca ccgaacaagc cgcgtttgtg
    24721 gtgttgaaat cgccgtcggt tccgtcggtg ctggtggaaa cctcgtttat caccaacccg
    24781 gaagaagagc ggctgttagg cactgcggcg tttcgccaga aaatcgccac agcgattgcc
    24841 gaaggcgtga tcagttattt ccactggttt gacaaccaga aagcacactc gagaaagcgg
    24901 taagtgatga aacccgacgc acaccaggtt aaacagtttc tgctcaacct tcaggatacg
    24961 atttgtcagc agttgagcgc cgtcgatggc gcagaatttg tcgaagatag ctggcagcgc
    25021 gaagctggcg gcggcgggcg tagtcgggtt ttgcgtaatg gtggtgtttt cgaacaggca
    25081 ggcgtcaact tttcacatgt ccacggtgag gcgatgcctg cttccgccac cgctcatcgt
    25141 ccggaacttg ccgggcgcag tttcgaggcg atgggcgttt cgctggtggt acatccgcat
    25201 aacccgtatg tccccaccag ccacgcgaat gtgcgctttt ttattgccga aaaaccgggt
    25261 gcagagcccg tctggtggtt tggcggcggc ttcgatttaa cccctttcta tggttttgaa
    25321 gaagacgcca ttcactggca ccgcaccgcc cgtgacctgt gcctgccatt tggcgaagac
    25381 gtttatccgc gttacaaaaa gtggtgcgac gaatacttct acctcaaaca tcgtaacgaa
    25441 cagcgcggca ttggcgggct gttctttgat gatctgaaca cgccagattt cgaccactgt
    25501 tttgccttta tgcaggcggt aggcaaaggc tacaccgacg cttatttgcc aattgtagag
    25561 cgacgtaaaa cgatggccta cggcgagcgc gagcgcaatt tccagttata tcgccgcggt
    25621 cgttatgtcg agttcaatct ggtctgggat cgcggaacgc tgtttggcct gcaaactggc
    25681 gggcgcaccg agtctatcct gatgtcaatg ccgccactgg tacgctggga gtatgattat
    25741 cagccagaag atggcagccc ggaagcggcg ttaagtgagt ttattaaggt cagggattgg
    25801 gtgtaactcc ctcaccccca ttcccgcatc cgctgatgca gcgtcagtga cggcttctcg
    25861 gcaaacagtt gctgataatc cgtggcaaat tgcccaagat gccagaatcc ccactgcatg
    25921 gcggcgtctt ttaccgtcat actttgcgac cacgggctta tcagttcgcg gcgtacagcg
    25981 ttcaggcgaa tgcgtttcag ccacgcattt gggccaatgc ctaaaatagc gtgaaacgcg
    26041 ttttgtagcg tgcggcggct gacatgcagt tgattacaca agtccagcac cgtcaccggt
    26101 tcggacatgt tttccagcac atattcacgg gcgcgggaaa gcaaccgacg gtaactctga
    26161 tgactgatgc tttccgccgt caccattggc tgcgcttctt ccagcatcgc ccccatcgcc
    26221 attagcaaat tatcccccag cactttacgc acagcaggct gatgaagatt ttccgggttc
    26281 tcgcaaaatg tcgccagcgc ctgttggaca aagccccaca gcgcggcttt atgctgctct
    26341 ttcacttcca acgccgactg gctacgcagc atatgtaata cccgatccgg attatgcaaa
    26401 aagttagcct gacgggtgat gacatcttca gaaagcacta cgccaaggat ggtgtaatcg
    26461 tccggcgtac ttaattcaaa ctcggttcca ccagggcgcg tcgctatttc tgcgcttccc
    26521 agacattgcg aaccgataaa tccctgctca ccgcgcgtcg ccggaatgcc aaaccagaac
    26581 gaattcggcc agaccaggca cgactgacgc agcgccagac cggtgtattc acgaaaaacc
    26641 tgaatatcat cgagtagaat ttccgtaaat tcaccatgaa acttgcccgg atgcagctga
    26701 tcgtaaatct gctgccaggc ggtaatcgtc aaagcatgtt catagacatc cgttgtctgt
    26761 cgttgatgaa cattatccac ttcgaccttc ggcgtgagct tcaggttttc gggtaagggt
    26821 tcatgataaa gatggtgcaa attggctgta cgggtctttt tcatgatgtt aatgccgggt
    26881 gttgtaggac acccggcacc tccgacaggt taatggggct tgagacggta acgactactg
    26941 cgtttacgta acgtcccggc agaaaagagc tgttcgagcg catttctggc tttttccagc
    27001 ggccagccaa agtgggcagc cacttctcct gccgtcattc cctgacgtac tgatgttaac
    27061 agcgccaaca actcgtcagc agattccgcg acgataactg gcgcgtcggg ttcctttacg
    27121 ggttgcttcg gctggcggtt aaagccagta accagccact gagtgtcatc gtctggccga
    27181 ccgatctcct tacggctgat gacacgcccg gtacgcatcg cggcagcaca acctgcgtcc
    27241 agcgccgcac gacacgccgc cagatcgcct tccaccacca gcgttaggcg accagggtca
    27301 agcacttcgt gactgagcag acgaacgtta gctgctttga gcatggcatc agctgcatct
    27361 atcgcggcga ccatgccgtc cacttccagc aatcccagtg cattgatcat cggcaacctc
    27421 cgttacgcac gctggattgg attacgcgcg atttccagca ctgcatcggt aaaggcgtta
    27481 caggctgctt tacacgcggc ctggctaccg gttaaaaatg ctgccgagta gttagtttca
    27541 gacggtggcg ggacataggt caccaactgc acgtcggcag atttcaacgc tgcatcaatg
    27601 ccgtaggtcg cttccagcgg cggtgccacc agatacgcca tcggatcgcc aagcgtgatc
    27661 ccggcggttg atgagagata agaaccggta cgcgaaacta catgtgccag gaaggctgtg
    27721 ttttctgcgt cattagccca ctggaaagca gcgccatttt caatattcgc aaccatcgca
    27781 tccagaccag cacgcacttc cgccgggttt ggtccaccaa gcataatcag cacctcaccg
    27841 gcagtcggtg acgggccgtg tgcagcgccc gcatacagcg agcgaccata taccacttcg
    27901 accatcgcct gcttggtcgc ttcgtcagcc gcaatgtagg tgacgtcatc tgaatcagca
    27961 gaaatcagcc cgaggctacg aatatgcggc ggcaatttca gttcacgtgc aaattcggcg
    28021 ttaacggagg caatcacccg catggcggtt accgacggtc gaatcaaatc taaagctggc
    28081 atgatgcctc cttatcgggt catgttgatg ccggatgctt tctgctccag catacgtttc
    28141 gccaaatcca caatgacggc tgcggcttca accggcggcg tgccgccctg gtgaatgtta
    28201 gagatacagg tgcgatcggc ctcaacggtg gtcgccatac gcggcgaata aacggcgtag
    28261 caggagaggc tttctgactg ccccaacccc ggacgttcgc ccaccagcag gatcactact
    28321 ttcgcgccga ggatctcacc gatctgatct tcaatcttca cgcgaccata acgtacaaag
    28381 aatggcgtac cgactttcag cccggcttgt ttcaggcccg ccatcagcgg cggcaggatc
    28441 tcttcgtagt tcacggtgat cgcatcagtt gacaggccat cagaaataac gacctgcacg
    28501 tccggattag caacacactg tgctttcagc gcttcaacag cttctgcaca caggcggcgg
    28561 cccatatccg ggcgagtcag atagagattt ttgtcgctga tttcggagcg gacttccagc
    28621 aagccctgcg ctttcaccca ctcttccggt acttctttca gtacggtatc tttcgaacga
    28681 gagtgatcgg ccaggaaacg cagcaacgcc tgggtacgcg gacgcggacc ggcacgaccg
    28741 gtacaaacgc gggccacggt gctgcggcgc agttctgtta atacgtccgc gcgatgtggg
    28801 ttttccacac caatccacgc ttttgcttcc gcagaaccta aatccagcgc gcagctttcc
    28861 gaggtcaccg gtgccgcaca gttggtggtg gcgcactttg cttctgacgg cgcgggggcc
    28921 gtttgtccca ttgacgccat cacactgcgt acaatttctt caatctgttt ttgatccatg
    28981 atgtgttatc tccgcgtcat cagaagaaca gtgacggatc gcccgcccgt ttggtcaggc
    29041 gaccgtttgc cataatgccc atgctttcca gccagcgttc aaactccggt gacggacgca
    29101 gattcagtaa ctgacgcaca gtggcagtgt cgtggaatgc cgtggtctga tagttgagca
    29161 tgatgtcatc gcccagcggc atccccatga tgtagttgca gcctgcggtg gcgagcagga
    29221 tcatgaggtt ttcgttgagg ttctggtcag cgtcagcgtg gttggtgtag cagcagtcac
    29281 agcccataga gatgccgctc agcttaccca taaagtgatc ttctaagccc gcacggataa
    29341 tctggcggtc gttgtagaga tactccggcc caataaagcc gaccacggtg ttgacgataa
    29401 acggatcgta atgacgcgcc agcccatagt tacgtgcttc catcgtcacc tggtctgcgc
    29461 cgaagttagc gccagcggac agcgcagagc cttgtccggt ttcgaagtag aggcagtttt
    29521 ccccggcgat acggttgaac tccgcgccca ctgcgcgcgc ttcatcgagc atcgccagct
    29581 ctacgccaaa ctctttcagc cctttttcgc tgccgcagat actctggaaa atcagcccgc
    29641 ctggtgcgcc acgacggatc gcttcgatct gggtggtaac gtgcgccagt acgcagccct
    29701 gagttgggat gttgaatttg tcgatcacgc catagatggt atccaacacg cggcttaagt
    29761 tttccacgtc gtcagtcacc gggttaacgc cgatcaccgc atcgcccacc ccgaaggaaa
    29821 gcccttcgta gatttgcgcg gcgatacttt gcacgtcgtc acgggtatca ttcggctgca
    29881 aacgggcgct aaaggtgccc ggaataccaa tggtggtatt ggcctttttg atcaccggca
    29941 ttttcttcgc gccgtagatc aggtccgcgt tggagcaaat cttcgctacc gccgccacca
    30001 cttccgaggt cagccctttg cgggtaaagg caatgtcgtc cacgctgatt tcatcgctca
    30061 gcacatactc acgcagttcg ctgatgctcc agtttttaat ctggttatag gccgtttcgt
    30121 taacgtcgtc ctgaatcagc cgcgtcacgc agtcatcttc ataggcaatc accggattat
    30181 tgcggatgtc cgctacggtc atttccgaca acacctgctt tgccgccacg cgctcctgtg
    30241 aacttgccgc agcaacgccc gccagcacat cccccgaacg cagttcgttg gctttagcca
    30301 gcacctcttt tacatcctta aactgatata cattgccgaa caatgtggtc tttagtttca
    30361 taagtcgttc cctcaggaag gaaatgcgag tgatttcacc gtcaccggca caaccgatcc
    30421 gccaaaaaga ggcgtaccaa tgtcgatata gtcccccgcg cggacaatca cttcgtcaat
    30481 gactgccaac gggagttgtt gtagctgtgg gcgcaacaac atgcccagag ctttaccaaa
    30541 gtcctgcccg gccaccacca gcaggggatg cggattcgga aaacgcgcga cgaaatcgac
    30601 cagcgcgttg atgaccgtca gtaccgcggc gtaacgcaca ggcagcgagg cgggaagcgc
    30661 cagcacgtac gcgtcagttt tggggcaaag atccagttga atcagcgcct gttgccaggc
    30721 actgaccaga tccgtttcat caatcgggat cgccactggc aaattgcgca gcggcagttg
    30781 tacgccctcc agccagattg tgctgccaga gagcgaaagg gtatgagcac ccgcgccaat
    30841 caccgtggcg cgcacggttt gcgccggaaa ctgcacgttc agctcacgca gacgcggatg
    30901 gtcatgcagc gccgttgcca gaagcgggcc gatatcggca aaacagaacg ggtcggcggg
    30961 ctggtggcga taacattcgc ctaccccgcc agaaagcgta atgatttcgg gcgtaacacc
    31021 tgcgggcagc aaatcggttt gcatcaatgc ctgcgcgagc ggcgagagcg ttccgtcaat
    31081 cacttcgaca atcagctctg ccatccggcg ggtaacctgc accagctgcc cgccagtcag
    31141 cgaacgggcg tcagtgcctg ggccgaagca ctcatccaca atcatctgcc ccggtttatg
    31201 agcgtaaacc acgcgcccct ggctgtcggt ttccagcagg cgaccgccga cgttgaggca
    31261 ggcagtcccg ctgatttttc cggcatcgaa cagggcgtag ttcgcggtgc caccgccgat
    31321 atcgatattc aggacccgac acagccgttg ttcagaaagg gtttgcgccc cggcaccgtg
    31381 accggcgatc acggattcga gatgcggtcc agcgctggcg acgacaaaat cgcccagcga
    31441 ccgggagagc gccatcaccg ccgggcgagc gttgcgggtt tttgcgcttt caccggtgat
    31501 aatgatggca ccagaatcaa cgctttccgg cgcaatgccc gcagcctgat attgctcgag
    31561 gattaaggtt ttcagttccg cttcttttaa accaccctgt ttatcgacag gggtaaagaa
    31621 caccgggctt tgccagctaa tttcgcgttt gatgaattcg tagcgcggca cctgcgacac
    31681 cgccgcacgg ttaaccagct ccaggtggga gaagatcacc tgggtggtgg tggtgccgat
    31741 atcgataccg acgctcagta gctggcgagt gttcacgatt gcgcctccgc ttcagtttta
    31801 gccgcggtcg cgtcttcttt tggcaccagc atcatcgcca cgccaattgc cgttacgccg
    31861 ccgatcaatt tgccgacaat catcgggaag atcatggcgt tcatgttggc agcggcgaag
    31921 cctaaatggt cgcccagggc gaaagcagcg gaaacggcga aggcgcagtt gatgactttg
    31981 ccgcgggtat ccatctgctt catcatgcca aacatcggaa tgttgttggc aagcgttgcc
    32041 accatgccgg ctgccgcgat attgttcata ttcagcacct taccgacgct catcagcggt
    32101 ttttcaaacc agcgagtcag cagcagtacc attggatacg cccctaacag aacgcaggag
    32161 atcgagccga taacttcaat ggcgcgcatc acttcaccgg gtttatcgcc aggggccata
    32221 aagataggat caagacccgg gatcagttcc cagccaagga ggaatttcac taccgcagcg
    32281 gcaagaccga gggtgatcaa tgcaacgagg aatttggcga agatctggaa gccgttgatc
    32341 attttttccg ggatgaattt cagccccagc gccaccagca ccgcaacgat aagtaccggg
    32401 atcatgttca tcaggatcag cgcaaaggtg aattcaacag gctggccgtt gatctgcaca
    32461 ccggagtaca tcgctaccag accaccagcg atacagccaa tcggaatggt cactatgccc
    32521 gccagcacgc cgagcgccag ataacgacgg tcagaaggtt cgataatgcc gagcgccacc
    32581 ggaatggaaa acacaatcgt tggccccatc atcgacccga gaattaaccc agagtatagc
    32641 cacgctgcta cgtcaccgcc cgccagctct ttggcgagga agaagccgcc catatcgcac
    32701 gccagcagtg ttccggcgaa cattgacggg tttgcgccga gaatttcgta aaccgggata
    32761 atcaccggtc cgagaacgtg ggccagtacc ggtgccagtg cggtcatacc gaccatcgcc
    32821 aggcccagag cgcccattgc cataaagcct tcttcgaact gaccgccgga tccttcgata
    32881 cttttaccga acttaccgag gaaacgagca gaaccgccga actgcgacag gatcctgtct
    32941 acggcagcta tcagcataaa gaacatcatg atgtacatga tgatttcgtt aatacccatc
    33001 gcctttactc cctgttagtt gttatttatt ggcggatgcg gcgtgaacgc cttatccgcc
    33061 ctacatgtgc aatcccgaag gccagataag acgcggca
//
LOCUS       DADSYM010000039        32048 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2415
            SAMN08039579-rid11723173.denovo.039, whole genome shotgun sequence.
ACCESSION   DADSYM010000039 DADSYM010000000
VERSION     DADSYM010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039579
            Sequence Read Archive: SRR6362533
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 32048)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32048)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980162.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 03:39:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,484
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 4,347
            CDSs (with protein)               :: 4,347
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 43 of 85
            Pseudo Genes (incomplete)         :: 37 of 85
            Pseudo Genes (internal stop)      :: 19 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32048
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039579-rid11723173.denovo.039"
                     /strain="LGE2415"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8302 N 95.9628 W"
                     /collection_date="2015-08-24"
     gene            complement(33..1712)
                     /gene="bcsG"
                     /locus_tag="J1Y83_001536"
     CDS             complement(33..1712)
                     /gene="bcsG"
                     /locus_tag="J1Y83_001536"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsG"
                     /protein_id="HBA7629693.1"
                     /translation="MTQFTQNTAMPSSLWQYWRGLSGWNFYFLVKFGLLWAGYLNFHP
                     LLNLVFAAFLLMPIPRYSLHRLRHWIALPIGFALFWHDTWLPGPESIMSQGSQVAGFS
                     TDYLIDLVTRFINWQMIGAIFVLFVAWLFLSQWIRITVFVVAILLWLNVLTLAGPSFS
                     LWPAGQPTTTVTTTGGNAAATVAATGGAPVVGDIPAQTAPPTTANLNAWLNNFYNAEA
                     KRKSIFPSSLPADAQPFELLVINICSLSWSDIEAAGLMSHPLWSHFDIEFKNFNSATS
                     YSGPAAIRLLRASCGQTSHTNLYQPANNDCYLFDNLSKLGFTQHLMMGHNGQFGGFLK
                     EVRENGGMQSELMDQTNLPVILLGFDGSPVYDDTAVLNRWLDVTEKDKNSRSATFYNT
                     LPLHDGNHYPGVSKTADYKARAQKFFDELDAFFTELEKSGRKVMVVVVPEHGGALKGD
                     RMQISGLRDIPSPSITDVPVGVKFFGMKAPHQGAPIVIDQPSSFLAISDLVVRVLDGK
                     IFTEDNVDWKKLTSGLPQTAPVSENSNAVVIQYQNKPYVRLNGGDWVPYPQ"
     gene            complement(1709..1900)
                     /gene="bcsF"
                     /locus_tag="J1Y83_001537"
     CDS             complement(1709..1900)
                     /gene="bcsF"
                     /locus_tag="J1Y83_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsF"
                     /protein_id="HBA7629694.1"
                     /translation="MMTISDIIEIIVVCALIFFPLGYLARHSLRRIRDTLRLFFAKPR
                     YVKPAGTLRRTEKARATKK"
     gene            complement(1897..3468)
                     /gene="bcsE"
                     /locus_tag="J1Y83_001538"
     CDS             complement(1897..3468)
                     /gene="bcsE"
                     /locus_tag="J1Y83_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsE"
                     /protein_id="HBA7629695.1"
                     /translation="MRDIVDPVFSIGISSLWDELRHMPAGGVWWFNVDRHEDAISLAN
                     QTIASQAETAHVAVISMDSDPAKIFQLDDSQGPGKITLFSMLNHEKGLYYLARDLQCS
                     IDPHNYLFILVCANNAWQNIPAERLRSWLDKMNKWSRLNHCSLLVINSGNNNDKQFSL
                     LLEEYRSLFGLASLRFQGDQHLLDIAFWCNEKGVSARQQLSVQQQNGCWTLVQHQEAE
                     IQPRSDEKRILSNVAVLEGAPPLSEHWQLFNNNEVLFNEARTAQAATVVFSLQQNAQI
                     EPLARSIHTLRRQRGSAMKILVRENTASLRATDERLLLACGANMVIPWNAPLSRCLTM
                     IESVQGQKFSRYVPEDITTLLSMTQPLKLRGFQKWDVFCNAVNNMMNNPLLPAHGKGV
                     LVALRPVPGIRVEQALTLCRPNRTGDIMTIGGNRLVLFLSFCRINDLDTALNHIFPLP
                     TGDIFSNRMVWFEDDQISAELVQMRLLAPEQWGMPLPLAQSSKPVINAEHDGRHWRRI
                     PEPMRLLDDAVERSS"
     gene            3741..3929
                     /locus_tag="J1Y83_001539"
     CDS             3741..3929
                     /locus_tag="J1Y83_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA7629696.1"
                     /translation="MNNNEPDTLPDPAIGYIFQNDILALKQAFSLPDIDYADISQREQ
                     LAAALKRWPLLAEFAQQK"
     gene            3941..4693
                     /gene="bcsQ"
                     /locus_tag="J1Y83_001540"
     CDS             3941..4693
                     /gene="bcsQ"
                     /locus_tag="J1Y83_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsQ"
                     /protein_id="HBA7629697.1"
                     /translation="MAVLGLQGVRGGVGTTTITAALAWSLQMLGENVLVVDACPDNLL
                     RLSFNVDFTHRQGWARAMLDGQDWRDAGLRYTSQLDLLPFGQLSIEEQENPQHWQTRL
                     SDICTGLQQLKASGRYQWILIDLPRDASQITHQLLSLCDHSLAIVNVDANCHIRLHQQ
                     ALPDGAHILINDFRIGSQVQDDIYQLWLQSQRRLLPMLIHRDEAMAECLAAKQPVGEY
                     RSDALAAEEILTLANWCLLNYSGLKTPVGSAS"
     gene            4690..7308
                     /gene="bcsA"
                     /locus_tag="J1Y83_001541"
     CDS             4690..7308
                     /gene="bcsA"
                     /locus_tag="J1Y83_001541"
                     /EC_number="2.4.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417990.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-forming cellulose synthase catalytic
                     subunit"
                     /protein_id="HBA7629698.1"
                     /translation="MSILTRWLLIPPVNARLIGRYRDYRRHGASAFSATLGCFWMILA
                     WIFIPLEHPRWQRIRAEHKNLYPHINASRPRPLDPVRYLIQTCWLLIGASRKETPKPR
                     RRAFSGLQNIRGRYHQWMNELPERVSHKTQHLDEKKELGHLSAGARRLILGIIVTFSL
                     ILALICVTQPFNPLAQFIFLMLLWGVALIVRRMPGRFSALMLIVLSLTVSCRYIWWRY
                     TSTLNWDDPVSLVCGLILLFAETYAWIVLVLGYFQVVWPLNRQPVPLPKDMSLWPSVD
                     IFVPTYNEDLNVVKNTIYASLGIDWPKDKLNIWILDDGGREEFRQFAQNVGVKYIART
                     THEHAKAGNINNALKYAKGEFVSIFDCDHVPTRSFLQMTMGWFLKEKQLAMMQTPHHF
                     FSPDPFERNLGRFRKTPNEGTLFYGLVQDGNDMWDATFFCGSCAVIRRKPLDEIGGIA
                     VETVTEDAHTSLRLHRRGYTSAYMRIPQAAGLATESLSAHIGQRIRWARGMVQIFRLD
                     NPLTGKGLKFAQRLCYVNAMFHFLSGIPRLIFLTAPLAFLLFHAYIIYAPALMIALFV
                     LPHMIHASLTNSKIQGKYRHSFWSEIYETVLAWYIAPPTLVALINPHKGKFNVTAKGG
                     LVEEEYVDWVISRPYIFLVLLNLVGVAVGIWRYFYGPPTEMLTVVVSMVWVFYNLIIL
                     GGAVAVSVESKQVRRSHRVEMTMPAAIAREDGHLFSCTVQDFSDGGLGIKINGQAQIL
                     EGQKVNLLLKRGQQEYVFPAQVARVMGNEVGLKLMPLTTQQHIDFVQCTFARADTWAL
                     WQDSYPEDKPLESLLDILKLGFRGYRHLAEFAPSSVKGIFRVLTSLVSWVVSFIPRRP
                     ERSETAQPSDQALAQQ"
     gene            7319..9658
                     /gene="bcsB"
                     /locus_tag="J1Y83_001542"
     CDS             7319..9658
                     /gene="bcsB"
                     /locus_tag="J1Y83_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis cyclic di-GMP-binding
                     regulatory protein BcsB"
                     /protein_id="HBA7629699.1"
                     /translation="MKRKIFWICAVALGMSAFPSFMTQATPATQPLINAEPAVAAQAE
                     QNPQVGQVMPGVQGADAPVVAQNGPSRDVKLTFAQIAPPPGSMVLRGINPNGSIEFGM
                     RSDEVVTKAMLNLEYTPSPSLLPVQSQLKVYLNDELMGVLPVTKEQLGKKTLAQMPIN
                     PLFITDFNRVRLEFVGHYQDVCENPASTTLWLDVGRSSGLDLTYQTLNVKNDLSHFPV
                     PFFDPRDNRTNTLPMVFAGAPDVELQQASAIVASWFGSRSGWRGQNFPVLYNQLPDRN
                     AIVFATNDKRPDFLRDHPAVKAPVIEMINHPQNPYVKLLVVFGRDDKDLLQAAKGIAQ
                     GNILFRGESVVVNEVKPLLPRKPYDAPNWVRTDRPVTFGELKTYEEQLQSSGLEPAAI
                     NVSLNLPPDLYLMRSTGIDMDINYRYTMPPVKDSSRMDISLNNQFLQSFNLSSKQEAN
                     RLLLRIPVLQGLLDGKTDVSIPALKLGATNQLRFDFEYMNPMPGGSVDNCITFQPVQN
                     HVVIGDDSTIDFSKYYHFIPMPDLRAFANAGFPFSRMADLSQTITVMPKTPNEAQMET
                     LLNTVGFIGAQTGFPAINLTVTDDGSTIQGKDADIMIVGGIPDKLKDDKQIDLLVQAT
                     ESWVKTPMRQTPFPGIVPDESDRAAETQSTLTSSGAMAAVIGFQSPYNDQRSVIALLA
                     DSPRGYEMLNDAVNDSGKRATMFGSVAVIRESGINSLRVGDVYYVGHLPWFERLWYAL
                     ANHPILLAVLAAISVILLAWVLWRLLRIISRRRLNPDNE"
     gene            9665..10771
                     /gene="bcsZ"
                     /locus_tag="J1Y83_001543"
     CDS             9665..10771
                     /gene="bcsZ"
                     /locus_tag="J1Y83_001543"
                     /EC_number="3.2.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulase"
                     /protein_id="HBA7629700.1"
                     /translation="MNVLRSGLVTMLLLAAFSVQAACTWPAWEQFKKDYISQEGRVID
                     PSDARKITTSEGQSYGMFFALAANDRAAFDNLLDWTQNNLAQGSLKEHLPAWLWGKKE
                     NSKWEVLDSNSASDGDVWMAWSLLEAGRLWKEQRYTDIGSALLKRIAREEVVTVPGLG
                     SMLLPGKVGFAEDNSWRFNPSYLPPTLAQYFTRFGAPWTTLRETNQRLLLETAPKGFS
                     PDWVRYEKDKGWQLKAEKTLISSYDAIRVYMWVGMMPDSDPQKARMLNRFKPMATFTE
                     KNGYPPEKVDVATGKAQGKGPVGFSAAMLPFLQNRDAQAVQRQRVADNFPGSDAYYNY
                     VLTLFGQGWDQHRFRFSTKGELLPDWGQECANSH"
     gene            10753..14226
                     /gene="bcsC"
                     /locus_tag="J1Y83_001544"
     CDS             10753..14226
                     /gene="bcsC"
                     /locus_tag="J1Y83_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026226.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsC"
                     /protein_id="HBA7629701.1"
                     /translation="MRKFTLNIFTLSLGLAVMPMVEAAPTAQQQLLEQVRLGEATHRE
                     DLVQQSLYRLELIDPNNPDVIAARFRSLLRQGDIDGAQKQLDRLSQLAPSSNAYKSSR
                     TTMLLSTSDGRQALQQARLQATTGHAEEAVASYNKLFNGAPPEGDIAVDYWSTVAKIP
                     ARRGEAINQLKRINADTPGNTGLQNNLALLLFSSDRRDEGFAVLEQMAKSNAGREGAS
                     KIWYGQIKDMPVSDASVQALKKYLSIFSDGDSVAAAQSQLAEQQKQLADPAFRARAQG
                     LAAVDSGMAGKAIPELQQAVRANPKDSEALGALGQAYSQKGDRANAVANLEKALALDP
                     HNSNNDKWNSLLKVNRYWLAIQQGDAALKANNPDRAERLFQQARNVDNTDSYAVLGLG
                     DVAMARKDYPAAERYYQQTLRMDSGNTNAVRGLANIYRQQSPEKAEAYIASLSASQRR
                     SIDDIERSLQNDRLAQQAEALENQGKWAQAAALQRQRLALDPGSVWITYRLSQDLWQA
                     GQRSQADTLMRNLAQQKPNDPEQVYAYGLYLSGHDQDRAALAHINSLPHAQWNSNIQE
                     LVNRLQNDQVLETANRLRENGKEAEAEAMLRQQPPSSRIDLTLADWAQQRRDYTAARA
                     AYQNVLTREPTNADAILGLTEVDIAAGDKAAARSQLAKLPATDNASLNTQRRVALAQA
                     QLGDTAAAQQTFNKLIPQAKSQPPSMESAMVLRDGAKFEAQAGDPTQALETYKDAMVA
                     SGVTTTRPQDNDTFTRLTRNDDKDDWLKRGVRSDAADLYRQQDLNVTLEHDYWGSSGT
                     GGYSDLKAHTTMLQVDAPYSDGRMFFRSDFVNMNVGSFSTNADGKWDDNWGTCTLQDC
                     SGNRSQSDSGASVAVGWRNDVWSWDIGTTPMGFNVVDVVGGISYNDDIGPLGYTVNAH
                     RRPISSSLLAFGGQKDSPSNTGKKWGGVRADGVGLSLSYDKGEANGVWASLSGDQLTG
                     KNVEDNWRVRWMTGYYYKVINQNNRRVTIGLNNMIWHYDKDLSGYSLGQGGYYSPQEY
                     LSFAIPVMWRERTENWSWELGASGLWSHSRTKTMPRYPLMNLIPTDWQEEAARQSNDG
                     GSSQGFGYTARALLERRVTSNWFVGTAIDIQQAKDYAPSHFLLYVRYSAAGWQGDMDL
                     PPQPLIPYADW"
     gene            14308..16296
                     /gene="hmsP"
                     /locus_tag="J1Y83_001545"
     CDS             14308..16296
                     /gene="hmsP"
                     /locus_tag="J1Y83_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417986.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator HmsP"
                     /protein_id="HBA7629702.1"
                     /translation="MRVSRSLTIKQMAMVAAVVLVFVFIFCTVLLFHLVQQNRYNTAT
                     QLESIARSVREPLSSAILKGDIPEAEAILASIKPAGVVSRADVVLPNQFQALRKSFIP
                     ERPVPVMVTRLFELPVQISLGVYSLERPANPQPIAYLVLQADSFRMYKFVMSTLSTLV
                     TIYLLLSLILTVAISWCINRLILHPLRNIARELNAIPPQELVGHQLALPRLHQDDEIG
                     MLVRSYNLNQQLLQRHYEEQNENAMRFPVSDLPNKALLMEMLEQVVARKQTTALMIIT
                     CETLRDTAGVLKEAQREILLLTLVEKLKSVLSPRMILAQISGYDFAVIANGVQEPWHA
                     ITLGQQVLTIMSERLPIERIQLRPHCSIGVAMFYGDLTAEQLYSRAISAAFTARHKGK
                     NQIQFFDPQQMEAAQKRLTEESDILNALENHQFAIWLQPQVEMTSGKLVSAEVLLRIQ
                     QPDGSWDLPDGLIDRIECCGLMVTVGHWVLEESCRLLAAWQERGIMLPLSVNLSALQL
                     MHPNMVADMLELLTRYRIQPGTLILEVTESRRIDDPHAAVAILRPLRNAGVRVALDDF
                     GMGYAGLRQLQHMKSLPIDVLKIDKMFVEGLPEDSSMIAAIIMLAQSLNLQMIAEGVE
                     TEAQRDWLAKAGVGIAQGFLFARPLPIEIFEESYLEEK"
     gene            16479..17765
                     /locus_tag="J1Y83_001546"
     CDS             16479..17765
                     /locus_tag="J1Y83_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate/amino acid:cation symporter"
                     /protein_id="HBA7629703.1"
                     /translation="MKTSLFKSLYFQVLTAIAIGILLGHFYPEIGEQMKPLGDGFVKL
                     IKMIIAPVIFCTVVTGIAGMESMKAVGRTGAVALLYFEIVSTIALIIGLIIVNVVQPG
                     AGMNVDPTTLDAKAVAVYADQAKDQGIVAFIMDVIPASVIGAFASGNILQVLLFAVLF
                     GFALHRLGSKGQLIFNVIESFSQVIFGIINMIMRLAPIGAFGAMAFTIGKYGVGTLVQ
                     LGQLIICFYITCILFVVLVLGSIAKATGFSIFKFIRYIREELLIVLGTSSSESALPRM
                     LDKMEKLGCRKSVVGLVIPTGYSFNLDGTSIYLTMAAVFIAQATNSQMDIVHQITLLI
                     VLLLSSKGAAGVTGSGFIVLAATLSAVGHLPVAGLALILGIDRFMSEARALTNLVGNG
                     VATIVVAKWVKELDHKKLDDVLNNRAPDGKTHELSS"
     gene            17985..19481
                     /locus_tag="J1Y83_001547"
     CDS             17985..19481
                     /locus_tag="J1Y83_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HBA7629704.1"
                     /translation="MQGTKIRLLAGGLLMMATAGYVQADALQPDPAWQQGTLSNGLQW
                     QVLTTPQRPSDRVEIRLLVNTGSLAESTQQSGYSHAIPRIALTQSGGLDAAQARSLWQ
                     QGIDPKRPMPPVIVSYDTTLFNLSLPNNRNDLLKEALSYLANATGKLTITPETINHAL
                     QSQDMVATWPADTKEGWWRYRLKGSTLLGHDPADPLKQPVEAEKIKDFYQKWYTPDAM
                     TLLVVGNVDARSVVDQINKTFGELKGKRETPAPVPTLSPLRAEAVSIMTDAVRQDRLS
                     IMWDTPWQPIRESAALLRYWRADLAREALFWHVQQALSASNSKDIGLGFDCRVLYLRA
                     QCAINIESPNDKLNSNLNLVARELAKVRDKGLPEEEFNALVAQKKLELQKLFAAYARA
                     DTDILMGQRMRSLQNQVVDIAPEQYQKLRQDFLNSLTVEMLNQDLRQQLSNDMALILL
                     QPKGEPEFNMKALQAAWDQIMAPSTAAASTSVATDDVHPEVTDIPPVQ"
     gene            complement(19577..20506)
                     /locus_tag="J1Y83_001548"
     CDS             complement(19577..20506)
                     /locus_tag="J1Y83_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HBA7629705.1"
                     /translation="MSKKIAVIGECMIELSEKGADVKRGFGGDTLNTSVYIARQVDPA
                     ALTVHYVTALGTDSFSQQMLDAWHGENVDTSLTQRMENRLPGLYYIETDSTGERTFYY
                     WRNEAAAKFWLESEQSAAICEELANFDYLYLSGISLAILSPTSREKLLSLLRECRANG
                     GKVIFDNNYRPRLWASKEETQQVYQQMLECTDIAFLTLDDEDALWGQQPVEDVIARTH
                     NAGVKEVVVKRGADSCLVSIAGEGLVDVPAVKLPKEKVIDTTAAGDSFSAGYLAVRLT
                     GGIAEDAAKRGHLTASTVIQYRGAIIPREAMPA"
     gene            20738..21505
                     /gene="pdeH"
                     /locus_tag="J1Y83_001549"
     CDS             20738..21505
                     /gene="pdeH"
                     /locus_tag="J1Y83_001549"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709303.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase"
                     /protein_id="HBA7629706.1"
                     /translation="MIRQVIQRISNPEASIESLQERRFWLQCERAYTWQPIYQTCGRL
                     MAVELLTVVTHPLNPSQRLPPDRYFTEITVSHRMEVVKEQIDLLAQKADFFVEHGLLA
                     SVNIDGPTLIALRQQPKILRQIEDLPWLRFELVEHIRLPKDSTFASMCEFGPLWLDDF
                     GTGMANFSALSEVRYDYIKIARELFVMLRQSPEGRTLFSQLLHLMNRYCRGVIVEGVE
                     TPEEWRDVQNSPAFAAQGWFLSRPAPIETLNTAVLAL"
     gene            21575..23635
                     /locus_tag="J1Y83_001550"
     CDS             21575..23635
                     /locus_tag="J1Y83_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HBA7629707.1"
                     /translation="MSKAGKITAAISGAFLLLIVVAIILIATFDWNRLKPTINQKVSA
                     ELNRPFAIRGDLGVVWERQKQETGWRSWVPWPHVHAEDIILGNPPDIPEVTMVHLPRV
                     EATLAPLALLTKTVWLPWIKLEKPDARLIRLSEKNNNWTFNLANDDNKDANAKPSAWS
                     FQLDNILFDQGRIAIDDKVSKADLEIFVDPLGKPLPFSEVTGSKGKADKEKVGDYVFG
                     LKAQGRYNGEPLTGTGKIGGMLALRGEGTPFPVQADFRSGNTRVAFDGVVNDPMKMGG
                     VDLRLKFSGDSLGDLYELTGVLLPDTPPFETDGRLVAKIDTEKSSVFDYRGFNGRIGD
                     SDIHGSLIYTTGKPRPKLEGDVESRQLRLADLGPLVGVDSGKGAEKSKRSEQKKGEKS
                     VQPAGKVLPYDRFETDKWDVMDADVRFKGRRIEHGSSLPISDLSTHIILKNADLRLQP
                     LKFGMAGGSIAANIHLEGDKKPMQGRADIQARRLKLKELMPDVELMQKTLGEMNGDAE
                     LRGSGNSVAALLGNSNGNLKLLMNDGLVSRNLMEIVGLNVGNYIVGAIFGDDEVRVNC
                     AAANLDIANGVARPQVFAFDTENALINVTGTASFASEQLDLTIDPESKGIRIITLRSP
                     LYVRGTFKNPQAGVKAGPLIARGAVAAALATLVTPAAALLALISPSEGEANQCRTILS
                     QMKK"
     gene            complement(23676..24998)
                     /locus_tag="J1Y83_001551"
     CDS             complement(23676..24998)
                     /locus_tag="J1Y83_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MHS family MFS transporter"
                     /protein_id="HBA7629708.1"
                     /translation="MQATATTLDHEQEYTPINSRNKVLVASLIGTAIEFFDFYIYATA
                     AVIVFPHIFFPQGDPTAATLQSLATFAIAFVARPIGSAVFGHFGDRVGRKATLVASLL
                     TMGISTVVIGLLPGYATIGIFAPLLLALARFGQGLGLGGEWGGAALLATENAPPRKRA
                     LYGSFPQLGAPIGFFFANGTFLLLSWLLTDEQFMSWGWRVPFIFSAVLVIIGLYVRVS
                     LHESPVFEKVAKAKKQVKIPLGTLLTKHVRVTVLGTFIMLATYTLFYIMTVYSMTFST
                     AAAPVGLGLPRNEVLWMLMMAVIGFGVMVPVAGLLADAFGRRKSMVVITTLIILFALF
                     AFNPLLGSGNPALVFVFLLLGLSLMGLTFGPMGALLPELFPTEVRYTGASFSYNVSSI
                     LGASVAPYIAAWLQTNYGLGAVGLYLAAMAGLTLIALLLTHETRHQSL"
     gene            complement(25391..26404)
                     /gene="yhjD"
                     /locus_tag="J1Y83_001552"
     CDS             complement(25391..26404)
                     /gene="yhjD"
                     /locus_tag="J1Y83_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YhjD"
                     /protein_id="HBA7629709.1"
                     /translation="MTQENEIKRPTQDLEHEPIKQLDNSEKGGKVSQALETVTTTAEK
                     VQRQPVIAHLIRATERFNDRLGNQFGAAITYFSFLSMIPILMVSFAAGGFVLASHPML
                     LQDIFDKILQNISDPTLAATLKNTINTAVQQRTTVGLVGLAVALYSGINWMGNLREAI
                     RAQSRDVWERSPQDQEKLWVKYLRDFISLIGLLIALIVTLSITSVAGSAQQMIISALH
                     LNSIEWLKPTWRLIGLAISIFANYLLFFWIFWRLPRHRPRKKALIRGTFLAAIGFEVI
                     KIVMTYTLPSLMKSPSGAAFGSVLGLMAFFYFFARLTLFCAAWIATAEYKDDPRMPGK
                     TQP"
     gene            complement(26453..27424)
                     /locus_tag="J1Y83_001553"
     CDS             complement(26453..27424)
                     /locus_tag="J1Y83_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBA7629710.1"
                     /translation="MLEKTINNAICALLFRCEQQSVKEMDKIHAMQLFIKVAELESFS
                     RAADFFALPKGSVSRQIQALEHQLGTQLLQRTTRRVKLTPEGMTYYQRAKDVLSNLSE
                     LDGLFQQDATSISGKLRIDIPSGIAKSLLLPRLSEFLYLHPGIELELSSHDRPVDILH
                     DGFDCVIRTGALPEDGVIARSLGKLTMVNCASPHYLTRFGYPQSPDDLTSHAIVRYTP
                     HLGVHPLGFEVASVNGVQWFKSGGMLTVNSSENYLAAGIAGLGIIQIPRIAVREALRA
                     GRLIEVLPGYRAEPLSLSLVYPQRRELSRRVNLFMQWLAGVMKEHLD"
     gene            27872..28474
                     /locus_tag="J1Y83_001554"
     CDS             27872..28474
                     /locus_tag="J1Y83_001554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBA7629711.1"
                     /translation="MQIVMFDRQSIFIHGMKISLQHRIPGVSIQGASQADELWQKLES
                     YPEALVMLDGDQDGEFCYRLLQKTVVQFPEVKVLITATDCNKRWLQEVIHFNVLAIVP
                     RDSTVETFALAVNSAAMGMMFLPGDWRTTPEKDIKDLKSLSARQREILTMLAAGESNK
                     EIGRALNISTGTVKAHLESLYRRLEVKNRTQAAMMLNISS"
     gene            complement(28525..30174)
                     /gene="treF"
                     /locus_tag="J1Y83_001555"
     CDS             complement(28525..30174)
                     /gene="treF"
                     /locus_tag="J1Y83_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalase TreF"
                     /protein_id="HBA7629712.1"
                     /translation="MLNQKIQNPNPDELMIEVDLCYELDPYELKLDEMIEAEPEPEMI
                     EGLPASDALTPADRYLELFEHVQSAKIFPDSKTFPDCAPKMDPLDILIRYRKVRRHRD
                     FDLRKFVENHFWLPEVYSSEYVSDPQNSLKEHIDQLWPVLTREPQDHIPWSSLLALPQ
                     SYIVPGGRFSETYYWDSYFTMLGLAESGREDLLKCMADNFAWMIENYGHIPNGNRTYY
                     LSRSQPPVFALMVELFEEDGVRGARRYLDHLKMEYAFWMDGAESLIPNQAYRHVVRMP
                     DGSLLNRYWDDRDTPRDESWLEDVETAKHSGRPPNEVYRDLRAGAASGWDYSSRWLRD
                     TGRLASIRTTQFIPIDLNAFLFKLESAIANISALKGEKETEALFRQKASARRDAVNRY
                     LWDDENGIYRDYDWRREQLALFSAAAIVPLYVGMANHEQADRLANAVRSRLLTPGGIL
                     ASEYETGEQWDKPNGWAPLQWMAIQGFKMYGDDLLGDEIARNWLKTVNQFYLEQHKLI
                     EKYHIADGVPREGGGGEYPLQDGFGWTNGVVRRLIGLYGEP"
     gene            30579..31976
                     /gene="ccp"
                     /locus_tag="J1Y83_001556"
     CDS             30579..31976
                     /gene="ccp"
                     /locus_tag="J1Y83_001556"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c peroxidase"
                     /protein_id="HBA7629713.1"
                     /translation="MKMVSRITAIGLAGVAICYLGLSGYVWYHDNKRSKQADVQASAV
                     SENNKVLGFLREKGCDYCHTPSAELPAYYYIPGAKQLMDYDIKLGYKSFNLEAVRAAL
                     LANKPVSQSDLNKIEWVMQYETMPPTRYTALHWAGKVSDEERAEILAWIAKQRAEYYA
                     SNDTAPEHRNEPVQPIPQKLPTDAQKVALGFALYHDPRLSADSTISCAHCHALNAGGV
                     DGRKTSIGVGGAVGPINAPTVFNSVFNVEQFWDGRAATLQDQAGGPPLNPIEMASKSW
                     DEIIAKLEKDPQLKAQFLEVYPQGFSGENITDAIAEFEKTLITPDSPFDKWLRGDENA
                     LTAQQKKGYQLFKDNKCATCHSGIILGGRSFEPLGLKKDFNFGEITAADIGRMNVTKE
                     ERDKLRQKVPGLRNVALTAPYFHRGDVPTLDGAVKLMLRYQVGKELPQEDVDDIVAFL
                     HSLNGVYTPYMQDKQ"
BASE COUNT         7707 a         8033 c         8744 g         7564 t
ORIGIN      
        1 cgcaggggga aactctgcgg cctttttcgt tcttactgcg ggtaaggcac ccagtcgccg
       61 ccgttcaggc gaacgtacgg tttattctgg tattgaataa ctactgcatt tgagttctcg
      121 gagaccggtg ctgtttgtgg taacccactg gtgagttttt tccagtcaac attgtcttcg
      181 gtgaaaatct tgccatcaag aacgcgaacc accagatcgg agatagccag gaagctgctc
      241 ggttggtcga tgacaatcgg agccccctga tgtggtgcct tcatgccgaa gaatttcacc
      301 ccaacgggga cgtctgtgat agacgggcta gggatatcac gtagaccaga tatctgcatt
      361 ctgtcgccct tcagcgcgcc gccgtgttcc ggcaccacta ccaccatcac tttacgaccc
      421 gatttctcca gttcagtaaa gaaggcgtcc agttcatcaa agaatttttg cgcccgcgct
      481 ttgtaatccg ctgttttgct gacgcccgga taatggttgc cgtcatgcag tggaagcgtg
      541 ttgtagaacg tggcactacg gctgttttta tctttttcgg taacgtccag ccaacggtta
      601 agcacggcgg tatcgtcata aaccggcgaa ccatcaaagc ccagcaaaat aaccggcaga
      661 tttgtttgat ccatcaattc gctctgcatg ccgccatttt cgcgaacttc cttcaaaaaa
      721 ccaccgaact ggccgttatg tcccatcatc aggtgctggg taaagcccag tttcgacagg
      781 ttatcaaaca gatagcagtc gttatttgcc ggttgataca gattagtgtg cgaagtctgc
      841 ccgcagctgg cgcgcagtaa acggatcgcc gccgggccac tgtaggaggt ggcggagtta
      901 aagttcttga actcaatatc gaaatgcgac cacagtggat gcgacatcaa tccggcggct
      961 tcaatatccg accaggaaag cgaacagata ttaatcacca gtagttcaaa tggctgagca
     1021 tcagcgggca gcgaagacgg gaagatcgat ttacgtttcg cctccgcgtt atagaaatta
     1081 ttcagccagg cgttaaggtt tgctgttgtt ggcggtgcag tttgtgcggg tatatcaccc
     1141 actaccggtg cgccacccgt cgccgcaacg gttgccgctg cgttaccacc cgtcgttgtt
     1201 acagtggtcg tcggttgacc ggctggccac aaggagaaac ttggtcccgc cagggtaagt
     1261 acgttcagcc atagcagaat ggcaaccaca aaaacggtaa tgcgaatcca ttgtgacagg
     1321 aataaccagg ccacgaataa aacaaaaatg gccccaatca tctgccagtt aataaagcgt
     1381 gtgacaagat cgattaaata atcggtactg aaccccgcca cctgcgaacc ctggctcatt
     1441 atgctttccg ggccaggcaa ccaggtgtca tgccagaaca aagcaaagcc gatcggcagg
     1501 gcaatccagt ggcgcaagcg atgcaggctg tagcgcggaa tgggcattag cagaaacgcg
     1561 gcaaacacca gattgaggag cggatggaag ttaagatatc ccgcccacaa caggccgaac
     1621 ttaaccagaa aatagaagtt ccagccggaa aggccgcgcc agtattgcca gagggaagaa
     1681 ggcatggcgg tattttgcgt aaattgagtc attttttggt tgccctggct ttttccgtgc
     1741 ggcgtaacgt cccggccggt ttaacataac gaggtttagc aaagaacaaa cgtaaggtgt
     1801 cgcgaatgcg tcgcaaagag tgccgcgcca gatagcccag cgggaaaaat atcagtgcgc
     1861 aaacgacaat aatttcaatg atatcgctga tggtcatcat gatgagcgct ccacagcatc
     1921 atctaacagt cgcattggtt ctggtattcg tcgccagtgg cgaccatcgt gctcggcatt
     1981 gatgaccggt ttagaacttt gcgctaaagg cagcggcatg ccccattgtt ctggggcaag
     2041 caggcgcatc tgcaccagct cggcactgat ttgatcatct tcaaaccaga ccattcggtt
     2101 tgagaaaatg tcgccagtag gcaatgggaa aatatgattc aacgcggtat ccagatcgtt
     2161 aatccgacag aatgagagaa acagcaccag ccgattaccg ccaatggtca tgatatcgcc
     2221 ggtacggtta gggcgacata gcgtcagggc ttgctcaacg cggatacccg gtaccggacg
     2281 tagggcaacc agaacgcctt taccgtgggc aggtaataga gggttattca tcatgttgtt
     2341 gacggcatta cagaacacat cccacttctg gaaacctcgc agtttgagcg gctgggtcat
     2401 tgacagcaag gtagtgatat cttccggcac atagcgacta aacttctgcc cttgcacgct
     2461 ttcgatcatc gtcagacagc gggagagtgg cgcattccac gggataacca tatttgcacc
     2521 gcaggccaat aataaacgtt catcggtggc gcgcaggctg gcggtatttt cccgtacgag
     2581 gattttcatc gcactaccgc gctggcgacg cagagtatga atgctgcggg ccagtggctc
     2641 gatttgcgca ttttgctgta aagaaaagac caccgtcgcc gcctgagcgg tacgggcttc
     2701 attgaacagg acttcgttat tgttgaacag ttgccagtgt tccgatagcg gcggcgcacc
     2761 ttccagtaca gcaacattac tcagaatgcg tttttcgtcg ctgcgtggtt ggatttccgc
     2821 ctcctggtgt tgaactaatg tccagcaacc attttgttgc tgaacgctaa gctgctgacg
     2881 ggcgctgacc cctttttcgt tgcaccagaa agcaatatcc agcaaatgtt ggtcgccctg
     2941 aaaacgcaaa ctggcaagac caaaaagtga acggtattcc tcaagtaaca atgaaaattg
     3001 tttatcgtta ttatttccgg aattaattac caaaagcgaa caatggttta gccggctcca
     3061 tttattcatt ttatccaacc atgagcgaag ccgctcggca ggaatgtttt gccatgcgtt
     3121 atttgcgcaa acaagaataa aaaggtaatt atggggatca atagaacact gcaaatcacg
     3181 ggccaaatag tatagacctt tttcatgatt tagcattgaa aataatgtta tttttcccgg
     3241 cccttgagaa tcatctaatt gaaagatttt cgctggatcg ctgtccatgc taatgaccgc
     3301 gacgtgtgcg gtttcagcct gggatgcaat tgtttgattc gccagactga tagcatcttc
     3361 atggcgatcg acgttaaacc accagacgcc gcctgctggc atatgtcgca gctcatccca
     3421 taatgatgag ataccgatag agaatacagg gtccacaatg tccctcatgt caacattttg
     3481 tctgattatc agtttactag cgaaagctaa gaaataaacc taccattgaa attaaaactt
     3541 atcagattta gcatgtgaat caatttagca ggtcacaaga catgtgattt tagtcttttc
     3601 gtttttaact ttttctgttg cgtaacggaa agtcaaaaag tgagcacatt cccgtctcgc
     3661 cgtaaacaat aaaccgacga aatgctcaca gttagaattg tttcatgaac ggtacggtta
     3721 tttcataggg atcaagaaaa atgaataaca atgaaccaga tactctgcct gatcccgcga
     3781 taggctatat cttccagaat gatattttgg cgttaaagca ggcattttca ctgcctgata
     3841 ttgattatgc cgatatttcc caacgcgaac agttggccgc ggcattaaaa cgctggccgt
     3901 tgctggcaga gtttgcgcaa caaaagtagg ggattggtga atggccgtac tgggattgca
     3961 gggggttcgg ggaggcgtgg ggacaacaac catcaccgcc gcattagcct ggtcattaca
     4021 aatgttggga gaaaatgtcc tggtggtcga tgcctgcccg gacaacttgt tgcgcctgtc
     4081 atttaacgtt gattttaccc accgtcaggg ttgggccaga gctatgctgg atggccagga
     4141 ctggcgtgac gccgggttgc gctatacctc gcagctcgat ttgctgcctt ttggtcagtt
     4201 atccattgaa gaacaagaaa atccacagca ctggcaaacc cggctgagcg atatttgcac
     4261 cggcttacag caactaaaag ccagcgggcg ttaccagtgg attttaattg acttaccgcg
     4321 tgatgcctcg cagataaccc accagctgct gagtttgtgc gatcactcgc tggcaatcgt
     4381 caatgtggat gccaactgcc atatccgact gcatcagcaa gcgctgccgg atggtgcaca
     4441 tattttgatt aatgacttcc gtattggcag ccaggttcag gacgatattt accagctttg
     4501 gttgcaaagc cagcgccgat tgctgccgat gctcattcat cgtgatgaag cgatggctga
     4561 atgtctggca gctaagcaac cagtcggtga atatcgcagt gatgcgctgg cggctgaaga
     4621 gatactgacg ctggcgaact ggtgcctgtt gaattattcc gggctgaaaa cgccagtcgg
     4681 gagtgcatca tgagtatcct gacccggtgg ttgcttatcc cgccggtcaa cgcacggctt
     4741 attgggcgtt atcgcgatta tcgtcgtcac ggtgcgtcgg ctttcagcgc gacgctcggc
     4801 tgtttctgga tgatcctggc ctggattttt attccgctgg agcacccgcg ctggcagcgt
     4861 attcgcgcag aacataaaaa cctgtatcca catatcaacg cctcgcgtcc gcgtccgctg
     4921 gacccggtcc gttatcttat tcaaacatgc tggttactga tcggtgcatc gcgcaaagaa
     4981 acgccgaaac cgcgcaggcg ggcattttca ggtctacaaa atattcgtgg acgttaccat
     5041 caatggatga acgagctgcc tgagcgcgtt agccataaaa cacagcatct tgatgagaaa
     5101 aaagagctcg gtcatttgag tgccggggcg cggcggttga tccttggtat catcgtcacc
     5161 ttctcgctga ttctggcgtt aatctgcgtt actcagccgt ttaacccgct agcgcagttt
     5221 atcttcctga tgctgctgtg gggggtagcg ctgatcgtac ggcggatgcc ggggcgcttc
     5281 tcggcgctga tgctgattgt gctgtcgctg accgtttctt gccgttatat ctggtggcgt
     5341 tacacctcga cgttaaactg ggacgatccg gtcagcctgg tgtgcgggct tattctgctg
     5401 ttcgccgaaa cgtacgcgtg gatagtgctg gtgctcggct acttccaggt cgtatggccg
     5461 ctgaatcgtc agccggtgcc attgccgaaa gatatgtcgc tgtggccgtc ggtggatatc
     5521 tttgtcccga cttacaacga agatctcaac gtggtgaaaa ataccattta cgcttcgctg
     5581 ggtatcgact ggccgaaaga caagctgaac atctggatcc tcgatgacgg cggcagggaa
     5641 gagtttcgcc agtttgcgca aaacgtgggg gtgaaatata tcgcccgtac cactcatgaa
     5701 catgcgaaag cgggcaacat caacaatgcg ctgaaatatg ccaaaggcga gttcgtgtcg
     5761 atattcgact gcgaccacgt accaacgcga tcgttcctgc aaatgaccat gggctggttc
     5821 ctgaaagaga aacagctggc gatgatgcag acgccgcacc acttcttctc gccggatccg
     5881 tttgaacgca acctggggcg ttttcgtaaa acgccgaacg aaggcacgct gttctatggt
     5941 ctggtgcagg acggcaatga tatgtgggat gccactttct tctgcggttc atgtgcggtg
     6001 attcgccgta agccgctgga tgagattggc ggaattgctg tcgaaacggt aaccgaagat
     6061 gcgcacactt ctctgcgtct gcaccgtcgt ggctacacct cagcgtatat gcgtattccg
     6121 caggcagcgg ggctggcgac cgaaagtctc tcggcgcata tcggtcagcg tattcgctgg
     6181 gcgcgcggga tggtgcaaat cttccgtctc gataacccgc tcaccggtaa agggctgaag
     6241 tttgctcagc gactgtgcta tgtcaacgcc atgttccact tcttgtcggg cattccacgg
     6301 ctgatcttcc tgactgcgcc gctggcgttc ctgctgtttc atgcctacat catctacgcg
     6361 ccagcgttga tgatcgccct gttcgtgcta ccgcatatga tccatgccag cctgactaac
     6421 tcgaagatcc agggcaaata tcgccactct ttctggagtg aaatctacga aacagtgctg
     6481 gcgtggtata tcgcaccacc gacgctggtg gcgctgatta acccgcacaa aggcaaattt
     6541 aacgtcaccg ccaaaggtgg actggtggaa gaagagtacg tcgactgggt gatctcgcgg
     6601 ccctacatct tccttgttct gctcaacctg gtgggcgtag cggttgggat ctggcgctac
     6661 ttctatggtc cgccaaccga gatgctcacc gtggtcgtca gtatggtgtg ggtattctac
     6721 aacctgatta ttctcggcgg cgcagtcgca gtatcggtag aaagcaaaca ggtacgccga
     6781 tcgcaccgcg tggagatgac gatgcccgca gcaattgccc gcgaagatgg tcacctcttc
     6841 tcgtgtaccg ttcaggattt ctccgacggt ggtttgggga tcaagatcaa cggtcaggcg
     6901 cagattctgg aagggcagaa agtgaatctg ttgcttaaac gcggtcagca ggaatacgtc
     6961 ttcccggctc aggtggcgcg cgtgatgggt aatgaagttg ggctgaaatt aatgccgctc
     7021 actacccagc aacatatcga ttttgtgcag tgtacgtttg cccgtgcgga tacatgggcg
     7081 ctctggcagg acagctaccc ggaagataag ccgctggaaa gtctgctgga tattctgaag
     7141 ctcggcttcc gtggctaccg ccatctggcg gaatttgcgc cttcttcggt gaagggcata
     7201 ttccgtgtgc tgacttctct ggtttcctgg gttgtatcgt ttattccgcg ccgcccggag
     7261 cggagcgaaa cggcacaacc atcggatcag gctttggctc aacaatgatg ataacgcgat
     7321 gaaaagaaaa atattctgga tttgtgcagt ggctctgggg atgagtgcgt tcccctcttt
     7381 catgacgcag gcgacgccag caacgcaacc actgatcaat gctgagccag ctgtagccgc
     7441 ccaggcggag caaaatccgc aggtggggca agtgatgccg ggcgtgcagg gcgcagatgc
     7501 gccagtcgtg gcgcagaacg gtccttcccg tgatgtgaag ctgacctttg cgcaaattgc
     7561 gccgccgccg ggcagcatgg tgctacgtgg cattaacccg aacggcagca ttgagtttgg
     7621 tatgcgcagc gatgaagtgg tgacgaaggc gatgctcaac ctcgaataca cgccatcgcc
     7681 atcattactg cctgtccagt cgcagttaaa ggtttatctc aatgatgaac tgatgggcgt
     7741 gctgccagtg accaaagaac agttgggtaa aaaaacgctg gcacaaatgc ccattaaccc
     7801 gctgtttatt actgatttca accgtgtgcg gctggagttt gtcggccatt atcaggacgt
     7861 gtgcgaaaac ccggccagca ccacgctttg gctggatgtt gggcggagca gtggactgga
     7921 tctgacctat cagaccctga atgtgaagaa tgacctgtca cacttcccgg tgccattctt
     7981 tgacccgcgt gataaccgca ccaacacctt gccgatggtc tttgcgggtg cgccggatgt
     8041 tgagctgcaa caagcatctg ccattgtcgc ctcgtggttt ggttcgcgtt caggctggcg
     8101 tgggcagaac ttcccggtgc tctataacca actgccggat cgcaatgcca ttgtctttgc
     8161 caccaatgac aaacgcccgg acttcctgcg cgatcatccg gcggtaaaag ccccggtgat
     8221 tgagatgatt aaccatccgc agaatcctta cgtcaaactg ctggtggtgt ttggccgtga
     8281 cgacaaagac ttgttgcagg cagcgaaagg tatcgctcag ggtaacattc tgttccgtgg
     8341 tgaaagtgtg gtagtgaatg aagtgaaacc gctgctaccg cgtaagccgt acgatgcgcc
     8401 gaactgggtg cgtaccgatc gtccggttac ctttggtgaa ctgaaaacct atgaagaaca
     8461 attacaatcc agcggtcttg agccagcagc gattaacgtt tcactaaacc tgccgccgga
     8521 tctctacctg atgcgcagta ccggcattga tatggatatt aattaccgct acaccatgcc
     8581 gccggtgaaa gacagttcgc ggatggatat cagcctgaat aaccagttcc tgcaatcctt
     8641 caacctgagc agcaaacagg aggcgaaccg cctgctgctg cggattccgg tattacaagg
     8701 tttgctggat ggcaaaacgg atgtctctat tccggcgctg aaactgggcg cgaccaacca
     8761 gctgcggttc gactttgagt atatgaaccc gatgccgggc ggttcggtgg ataactgtat
     8821 taccttccag ccggtgcaga atcatgtggt gattggtgac gactccacca tcgacttctc
     8881 gaagtattac cacttcatcc cgatgccgga tctacgcgcc tttgctaacg cgggcttccc
     8941 gttcagccgg atggcggatt tgtcgcaaac catcaccgtg atgccgaaaa cgccaaacga
     9001 agcacagatg gaaaccttgc tgaataccgt tggctttatc ggtgcgcaga cgggcttccc
     9061 ggcgattaac ctgacagtga ccgatgatgg cagcaccatt cagggcaaag atgccgacat
     9121 catgatcgtc ggtggtatcc cggacaaact gaaagatgac aagcagatcg acctgttggt
     9181 gcaggcgacc gaaagctggg taaaaacgcc gatgcgccag accccgttcc ccggtattgt
     9241 gccggacgag agcgatcgcg cggcagaaac ccagtcaacg ctgacctctt ccggtgcgat
     9301 ggcggcggtg attggcttcc agtcgccgta taacgaccag cgcagcgtga ttgcgctgct
     9361 ggcagatagc ccacgcggtt atgaaatgct taacgatgcg gtgaacgata gcggcaaacg
     9421 cgccaccatg ttcggttcgg tcgcggtgat ccgcgagtcc ggtatcaaca gcctacgtgt
     9481 tggcgatgtt tattacgtag gtcatctgcc gtggttcgag cgcttgtggt atgcgctggc
     9541 aaaccatccg attctgctgg cggtgctggc ggctatcagt gtgatattgc tggcgtgggt
     9601 actgtggcgt ctgctgcgta ttattagtcg tcgtcgtctt aacccggata acgagtaatt
     9661 gaagatgaat gtgttgcgta gtggactcgt gacgatgctg ctgctggctg cctttagtgt
     9721 tcaggcagcc tgtacctggc ctgcctggga gcagtttaaa aaggattaca tcagtcagga
     9781 agggcgcgtc atcgacccca gcgacgcgcg caaaatcacc acctccgaag ggcaaagtta
     9841 cggcatgttc tttgccctgg cggctaacga ccgtgcagct ttcgataatc ttctcgactg
     9901 gacgcagaac aatctcgctc agggttcttt aaaagaacat ttgcccgcct ggctgtgggg
     9961 caagaaagag aacagtaagt gggaagtgct ggacagcaat tcggcctccg atggtgatgt
    10021 ctggatggcc tggtcgttgc tggaggcggg gcgtttgtgg aaagagcagc gttataccga
    10081 catcggcagc gcattgctaa aacgtatcgc gcgggaggaa gtggtgacgg tgcctgggct
    10141 gggctccatg ttgttaccgg gcaaagtggg ttttgctgag gataacagct ggcgttttaa
    10201 ccccagctac ctgccgccga cgctggcgca gtatttcacc cgctttggcg cgccgtggac
    10261 cacgctgcgc gaaaccaatc aacgtttatt gctggaaacc gccccgaaag gcttttcgcc
    10321 agactgggtg cgctatgaga aagacaaagg ctggcagcta aaagccgaaa aaacattgat
    10381 cagcagctac gacgctatcc gcgtttacat gtgggtaggc atgatgcctg acagcgatcc
    10441 gcaaaaagcg cggatgctca accggtttaa accgatggcg acattcactg agaaaaacgg
    10501 ttatccgccg gaaaaagtgg atgtggctac ggggaaagcg cagggtaaag gaccggtcgg
    10561 tttttctgcc gccatgctgc cctttttaca aaaccgcgat gcgcaggccg ttcagcgcca
    10621 gcgcgtggcc gataactttc ccggcagcga tgcctattac aactatgtgc tgaccctgtt
    10681 tggacaaggc tgggatcaac accgtttccg cttctcgaca aaaggtgagt tattacctga
    10741 ctggggccag gaatgcgcaa attcacacta aacatattca cgctttccct cggtctggcc
    10801 gtcatgccga tggtcgaggc agcaccaacc gctcagcaac agttgctgga gcaagttcgg
    10861 ttaggcgaag cgacccatcg tgaagacctg gtgcaacagt cgttgtatcg gctggaactt
    10921 attgatccga ataacccgga tgtgattgct gcccgttttc gttctctgtt acgtcagggc
    10981 gatattgatg gcgcacaaaa acagctcgat cggctgtcgc agttagcgcc gagttcaaat
    11041 gcgtataaat cgtcacggac tacgatgctg ctttccacgt cggatggtcg tcaggcactg
    11101 caacaggcaa gattgcaggc gacgactggt catgcggaag aagctgtggc gagttacaac
    11161 aaactgttca acggtgcgcc gccggaaggt gacattgctg tcgattactg gagtacggtg
    11221 gcgaaaattc cggcccgccg tggcgaagcg attaatcagc taaaacgcat taatgcggat
    11281 acgccgggca acacgggcct gcaaaataat ctggcgctat tgctgtttag tagcgatcgc
    11341 cgtgatgaag gttttgccgt cctggaacag atggcgaaat cgaacgccgg gcgcgaaggg
    11401 gcctctaaaa tctggtacgg gcagattaaa gacatgcccg tcagcgatgc cagcgtgcag
    11461 gcactgaaaa aatatctctc gatctttagc gatggcgata gcgtagctgc tgcacaatcg
    11521 caactggcag aacagcaaaa acagctggcc gaccctgctt tccgcgctcg tgcgcaaggt
    11581 ttagcggcgg tggactctgg catggcgggt aaagccattc ccgaactaca acaggcggtg
    11641 cgggcgaacc cgaaagacag tgaggctctg ggggcgctgg gccaggcgta ttcccagaaa
    11701 ggtgatcgcg ccaatgcagt ggcgaatctg gaaaaagccc tcgcgctgga tccgcacaac
    11761 agcaataacg acaaatggaa cagtctgctg aaagtaaacc gctactggct ggcgatccag
    11821 cagggcgatg ccgcgctgaa agccaataat cctgaccggg cagaacggct gttccagcag
    11881 gcacgtaatg tcgataacac cgacagctat gcagtgcttg ggctgggcga tgtggcgatg
    11941 gcgcgcaaag attatcccgc cgccgaacgc tattaccagc agaccttgcg tatggacagc
    12001 ggcaacacta acgccgtgcg cgggctggcg aatatttacc gccagcagtc gccagaaaaa
    12061 gccgaagcgt atatcgcctc gctctctgcc agccagcggc gcagcattga tgatatcgaa
    12121 cgcagcctgc aaaacgaccg tctggcacag caggcagagg cactggaaaa ccaggggaaa
    12181 tgggcgcagg cggcagcact tcagcggcaa cgactggcgc tggaccccgg cagtgtatgg
    12241 attacttacc gactttcgca ggatctctgg caagccggac aacgcagcca ggccgatacg
    12301 ttaatgcgca atctggcgca gcagaagccg aacgacccgg agcaggttta cgcttacggg
    12361 ctgtacctct ccggtcatga tcaggacaga gcggcgctgg cgcatatcaa cagcctgccg
    12421 cacgcgcagt ggaacagcaa tattcaggag ctggttaatc gactgcaaaa cgatcaggtg
    12481 ctggaaaccg ctaatcgcct gcgagaaaac ggtaaagagg cagaagcgga agcgatgctg
    12541 cgccagcaac cgccttcctc gcgtattgat ctgacgctgg ctgactgggc gcagcagcga
    12601 cgtgattaca ccgccgcccg cgctgcatat cagaatgttc tgacgcggga gccaactaac
    12661 gccgatgcca ttcttggtct gacggaagtg gatattgctg ccggtgacaa agcggcggca
    12721 cgtagccagc tggcgaaact gcctgccact gataacgcct cgctgaacac acagcggcgc
    12781 gtggcgctgg cacaggcgca gcttggcgat accgcagcgg cgcagcagac atttaataag
    12841 ttgatcccgc aggcaaaatc tcagccaccg tcgatggaaa gcgcgatggt actgcgtgat
    12901 ggtgcgaagt ttgaagcgca ggcgggcgat ccaacgcagg cgctggaaac ctacaaagac
    12961 gccatggttg catccggtgt gaccacgacg cgtccgcagg ataacgacac ctttacccgg
    13021 ctgacccgta acgacgataa agatgactgg ctgaaacgcg gcgtgcgcag cgatgcggcg
    13081 gacctctatc gccagcagga tcttaacgtc actctcgagc acgattactg gggttcgagc
    13141 ggtaccggtg gttactccga tctgaaagcg cacaccacta tgttgcaggt ggacgcgccc
    13201 tattctgacg ggcggatgtt ctttcgcagt gatttcgtca atatgaacgt cggcagtttc
    13261 tccactaatg ccgatggcaa atgggatgac aactggggca cctgtacatt acaggactgt
    13321 agcggcaacc gcagccagtc ggattccggt gccagcgtgg cggtcggctg gcgaaatgac
    13381 gtctggagct gggatatcgg taccacgccg atgggcttca acgtggtgga tgtggtcggc
    13441 ggcatcagtt acaacgatga tatcgggccg ctgggttaca ccgttaacgc ccaccgtcgg
    13501 cccatctcca gttctttgct ggcctttggt gggcaaaaag actctccgag caataccggg
    13561 aaaaaatggg gcggcgtgcg tgccgacggc gtggggctaa gtctgagcta cgataaaggt
    13621 gaagcaaacg gcgtctgggc atcgcttagc ggtgatcagt taaccggtaa aaatgtcgaa
    13681 gataactggc gcgtgcgctg gatgacgggc tattactata aggtcatcaa ccagaacaat
    13741 cgccgcgtca caatcggcct gaacaacatg atctggcatt atgacaaaga cctgagtggc
    13801 tactcactcg gacagggcgg ttactacagt ccgcaggaat acctgtcgtt tgccataccg
    13861 gtgatgtggc gggagcgcac ggaaaactgg tcgtgggagc tgggggcgtc tggcttgtgg
    13921 tcgcattcac gcaccaaaac catgccgcgt tatccgctga tgaatctgat cccgaccgac
    13981 tggcaggaag aagctgcgcg gcaatccaac gatggcggca gcagtcaggg ctttggctac
    14041 acggcgcggg cattacttga acgacgtgtt acttccaact ggtttgtcgg cacggcaatt
    14101 gatatccagc aggcaaaaga ttacgcaccc agccatttcc tgctctacgt acgttattcc
    14161 gccgccggat ggcagggtga catggattta ccgccgcagc cgctgatacc ttacgccgac
    14221 tggtaagttt tcagatagcg cttctcttaa tgccgctgcg atcgggtata ctcgggcggc
    14281 aatctgggat ttccgggggg agacaatttg cgcgtaagtc gctcgttaac aatcaagcag
    14341 atggcaatgg tggcagccgt tgtcctggtg ttcgttttta ttttttgcac cgttttgctg
    14401 ttccatctgg tccagcagaa tcgctataac acggctacgc aactggaaag cattgctcgc
    14461 tctgtccgcg aacccttatc ttctgccatt ttgaaaggcg atattcccga agcggaagct
    14521 attcttgcca gcattaaacc ggcaggcgtg gtcagccgtg ccgatgtagt gctgcctaac
    14581 cagttccagg cgctgcgtaa aagttttatt ccagagcgtc cggtgccggt aatggttact
    14641 cgcctgtttg agctaccggt gcaaatctcg ctgggcgttt actcgctgga acgtccggca
    14701 aacccgcagc caattgccta tctggtgtta caggcggatt ccttccgcat gtataagttc
    14761 gtgatgagca ccctctcaac gttagtgacc atttacttac ttttgtcgtt aatattgacg
    14821 gtggcgatta gctggtgcat taaccgcctg attttgcatc cgttgcgcaa tattgctcgc
    14881 gaacttaacg ccatcccacc ccaggagctt gttggtcacc aactggcatt accgcgtctg
    14941 catcaggacg atgaaatcgg tatgttggtg cgcagttaca acctcaacca gcaattgctg
    15001 cagcgccatt atgaagaaca gaacgaaaat gcgatgcgct tcccggtgtc ggatttgccg
    15061 aacaaagcct tgctgatgga gatgctggag caggtggtgg cgcgtaaaca aaccaccgcg
    15121 ctgatgatca tcacctgtga aaccctgcgt gatactgcgg gtgtgctgaa agaggcgcaa
    15181 cgagaaattc tgctgctgac gctggtggaa aaactcaaat cggtactgtc gccacgcatg
    15241 atcctcgcgc agattagcgg ttatgacttt gctgtcattg ccaacggtgt acaggaaccg
    15301 tggcacgcta ttacattagg tcagcaagtg ctcactatca tgagcgagcg cctgccgatt
    15361 gaacgtattc aactccgtcc gcactgtagc attggcgtgg cgatgttcta cggcgatctc
    15421 accgccgaac agctttacag tcgcgctatt tctgcggcat ttaccgctcg ccataaaggc
    15481 aaaaatcaga ttcagttctt tgatccgcag cagatggaag ccgcccagaa gcggttgacg
    15541 gaagagagcg atatccttaa tgcactggaa aatcatcagt ttgcgatttg gttacagcca
    15601 caggtcgaga tgaccagcgg taaactggtc agtgcggaag tgttactgcg tatccagcaa
    15661 ccggatggca gttgggacct gcccgatggc ttaatcgatc gcattgagtg ctgtgggctg
    15721 atggttaccg tcggtcactg ggtgctggaa gagtcctgtc gattgcttgc tgcctggcaa
    15781 gagcgcggca ttatgctgcc cttgtcggta aacctctcag cgctgcaact gatgcatcca
    15841 aatatggtgg cggatatgct ggaactgtta acccgctatc gcattcagcc gggaacactg
    15901 attctggaag tgacagaaag ccgacgtatt gacgatcctc atgctgcggt ggcaatcctc
    15961 cgtccgctgc gcaatgccgg agttcgggtg gcgctggatg atttcggcat gggctacgca
    16021 gggctgcgtc agctgcagca tatgaaatcg ttgccaatcg acgtactgaa aatcgacaaa
    16081 atgtttgttg aaggtttgcc ggaagatagc agcatgattg ctgcaattat catgctggcg
    16141 cagagcctga acttacaaat gattgccgaa ggcgtggaga ctgaagcaca acgcgactgg
    16201 ctggcaaaag cgggcgttgg tattgcccag ggcttccttt ttgctcgccc actccctatt
    16261 gaaatcttcg aagagagtta cctggaagaa aagtagctac cccaaactga ttacaaaact
    16321 ttaaaaagtg ctggtttgtg cgagccagct caaacttttt aacctttttg tttcaattat
    16381 gatccaggta catttctgtg atgttgtctg agtgttattt taaggccgca ggtaccccat
    16441 aaccttacaa gacctgtggt tttactaaag gacaccctat gaaaacctct ctgtttaaaa
    16501 gcctttactt tcaggtcctg acagcgatag ccattggtat tctccttggc catttctatc
    16561 ctgaaatagg cgagcaaatg aaaccgcttg gcgacggctt cgttaagctc attaagatga
    16621 tcatcgctcc tgtcatcttt tgtaccgtcg taacgggcat tgcgggcatg gaaagcatga
    16681 aggcggtcgg tcgtaccggc gcagtcgcac tgctttactt tgaaattgtc agtactatcg
    16741 cgctgattat tggtcttatc atcgttaacg tcgtgcagcc tggtgccgga atgaacgtcg
    16801 atccgacaac gcttgatgcg aaagcggtag cggtttacgc cgatcaggcg aaagaccagg
    16861 gcattgtcgc cttcattatg gatgtcatcc cggcgagcgt cattggcgca tttgccagcg
    16921 gtaacattct gcaggtactg ctgtttgccg tactgtttgg ttttgcgctc caccgtctgg
    16981 gcagcaaagg ccaactgatt tttaacgtga tcgaaagttt ctcgcaggtc attttcggca
    17041 tcatcaatat gatcatgcgt ctggcaccta ttggtgcatt cggggcaatg gcgtttacca
    17101 tcggtaaata cggcgtcggc acactggtgc aactggggca gctgattatc tgtttctaca
    17161 tcacctgtat cctgtttgtg gtgctggtat tgggttcaat cgctaaagcg actggcttca
    17221 gtatcttcaa atttatccgt tacatccgtg aagaactgct gattgtactg gggacttcat
    17281 cttccgagtc ggcgctgccg cgtatgctcg acaagatgga gaaactcggc tgccgcaaat
    17341 cggtggtggg gctggtcatc ccgacaggct actcgtttaa ccttgatggc acatcgatat
    17401 acctgacaat ggcggcggtg tttatcgccc aggccactaa cagtcagatg gatatcgtcc
    17461 accaaatcac gctgttaatc gtgttgctgc tttcttctaa aggggcggca ggggtaacgg
    17521 gtagtggttt tatcgtgctg gcagcgacgc tctctgcggt gggccatttg ccggtagcgg
    17581 gtctggcgct gatcctcggt atcgaccgct ttatgtcaga agctcgtgcg ctgactaacc
    17641 tggtcggtaa cggcgtagcg accattgtcg ttgctaagtg ggtgaaagaa ctggaccaca
    17701 aaaaactgga cgatgtgctg aataatcgtg cgccggatgg caaaacgcac gaattatcct
    17761 cttaatctca ccacttatgc ccgcactccc ttccccgagt gcgggtgttt gcgcataatt
    17821 gacccctgtg cctgaactta ccacctgggt gagttcaggt ttatttcact tcttccgtga
    17881 cattttcatg ttcttgcggt ctaacacgaa gtgtttttaa cgtcatttag actgaccgac
    17941 aacgggcagt tttttattac caggatagtt gatcaggggt tcacatgcag ggcacaaaaa
    18001 ttcgactttt agcgggcggt ttgctgatga tggccactgc tggctatgtg caggcagatg
    18061 cgctccagcc tgatccagca tggcaacagg ggacgctttc caacggttta cagtggcaag
    18121 tgctgactac gccccagcgt cccagcgatc gtgttgaaat tcgcctgctg gttaataccg
    18181 gttcgctcgc cgaaagtaca caacagagcg gttacagtca cgccatccct cgtattgcgc
    18241 taacgcaaag cggtggcctt gacgcagcgc aggcgcgttc attgtggcag caggggatcg
    18301 accctaaacg cccgatgccg ccggtaattg tctcttatga caccacgctg tttaatctga
    18361 gtttgcccaa taaccgtaac gacttgctga aagaggcgct ctcttatctg gcaaatgcca
    18421 ctggcaaact gactatcacg ccagaaacca tcaaccatgc gttgcaaagt caggacatgg
    18481 tggcaacctg gcctgccgat actaaagagg gctggtggcg ttatcgtctg aaaggatcaa
    18541 ccttgttagg tcacgatcct gccgatccgc tgaaacaacc cgttgaagcg gaaaagatta
    18601 aagatttcta tcagaaatgg tacaccccgg atgcaatgac gctgctggtg gtgggaaacg
    18661 tggatgcgcg ctcggtcgtc gaccaaatca ataaaacgtt tggcgaactg aaaggcaaac
    18721 gtgaaacacc ggctccggtg ccgacgcttt ctccgctgcg tgcggaagcg gtgagtatta
    18781 tgaccgacgc ggtgcgtcag gaccggctat ctatcatgtg ggatacgccg tggcagccga
    18841 ttcgtgaatc agccgcactg ctgcgctact ggcgtgcgga cctggcccgt gaggcgctgt
    18901 tctggcatgt tcagcaagcg ttaagcgcca gtaacagtaa agacatcggt cttggatttg
    18961 actgtcgtgt gctgtatctg cgtgcgcagt gtgccatcaa catcgaatca ccaaacgaca
    19021 agctgaacag caaccttaat ctggtggcgc gtgaactggc gaaggttcgc gataaaggtc
    19081 tgccggaaga agagttcaat gcgttagtgg cgcaaaagaa actggagctg cagaaactgt
    19141 ttgccgccta tgcgcgagct gataccgata ttctgatggg gcagcggatg cgttcgttgc
    19201 aaaatcaggt cgtcgatatc gcgccggagc aatatcagaa actgcgtcag gatttcctta
    19261 acagcctgac ggtggagatg ttaaatcagg atctgcgtca gcagttgtcg aatgatatgg
    19321 cgttaatact gctgcagccg aaaggcgagc cggaatttaa catgaaagcg ttgcaggcgg
    19381 cctgggatca aatcatggcc ccatcgactg cggctgcctc cacatctgtc gccacggatg
    19441 acgtacatcc tgaagtgacg gatattccac ctgtacagta atgcctgatg gtgatgagtt
    19501 agcacaacca tcaggtattt atggatgagc tggatagggg ggtaacactc tatccagcct
    19561 tttgcatatg ctgcttttac gctggcatcg cttcacgcgg gataatcgcg ccgcgatact
    19621 gaataacggt acttgcggtc aggtgcccgc gtttcgctgc gtcttccgca atgccgcccg
    19681 tcaaacgtac cgccagataa ccggcactga aagagtcgcc agctgcggtg gtatcgatca
    19741 ctttttcttt cggcagtttt accgccggaa catccactaa cccttcacca gcaatggaca
    19801 ccaggcaaga atccgccccg cgtttcacca ccacttcttt cacgcccgcg ttatgggtgc
    19861 gcgcaatgac gtcttccacc ggctgttgac cccacagcgc gtcttcatcg tccagcgtca
    19921 ggaaggcgat atccgtgcat tccagcattt gttggtacac ctgctgtgtc tcttctttgc
    19981 tggcccacag gcgcggacga tagttattgt cgaaaatcac ttttccgccg ttggcgcggc
    20041 attcgcgcag cagggaaagc agcttttcgc ggctggtcgg gcttaagatc gccaggctaa
    20101 tcccgctcag gtagagataa tcgaaattcg ccagctcttc gcaaatcgcc gcggattgct
    20161 cactctccag ccagaatttg gcggcggctt cgttacgcca gtagtagaac gtacgttcgc
    20221 cggtgctgtc ggtttcaatg tagtaaaggc ccggcagacg gttttccatc cgttgggtca
    20281 gggaagtatc aacgttctcg ccgtgccagg cgtccagcat ctgctggcta aaactgtccg
    20341 ttcccagcgc cgttacgtaa tgaacggtta atgccgcagg atcgacctga cgggcgatat
    20401 agacggaagt gttcagggta tcgccgccga aaccgcgctt aacgtccgcg cctttctcgg
    20461 aaagctcaat catgcattcg ccaatcacgg caatcttttt ggacatagtc gtgaacctga
    20521 tctgtaaaaa attagcgtta gtttgcgctg tggtggtttg ctggtcaacc atattaaaac
    20581 agtgttccat tatttttttg agccagaacg tattcctgaa agattccgtt gtggagtggg
    20641 gaaatttacc ccggccaggc ggtaaagttc tgcccttgcg cgccgataat ctttgtcgag
    20701 tccgggcagc atcactttta aacacaggac atctttgatg ataaggcagg ttatccagcg
    20761 aataagcaac cctgaagcaa gcatcgagag cttgcaggaa cggcgttttt ggttgcagtg
    20821 tgagcgtgct tacacctggc agccaatcta tcaaacatgc gggcggttaa tggccgtgga
    20881 gctattaacg gtggtcacgc atcccttgaa cccttcgcaa cgcctgccgc cggatcgcta
    20941 ttttactgaa atcaccgtta gccatcggat ggaggttgtg aaagagcaga ttgatttgct
    21001 ggcgcaaaaa gccgacttct ttgtagagca cggcctgctg gcatcggtca atattgatgg
    21061 ccctacgctc atcgccctgc gtcagcaacc aaaaatcctg cgccagattg aggatcttcc
    21121 ctggctgcgt ttcgaactgg tggaacacat ccgcctgccg aaagattcaa cctttgcctc
    21181 gatgtgtgaa tttggcccgc tgtggctgga tgattttggt accgggatgg caaatttctc
    21241 tgcactaagt gaagtgcgtt atgactacat caaaatcgcg cgagaactgt ttgtgatgct
    21301 gcgtcagtca ccggaaggac gcacactctt ttctcagctt ttacatctaa tgaatcgcta
    21361 ttgtcgcggg gtgattgtcg agggcgtaga aacaccagaa gagtggcgtg atgttcagaa
    21421 ctcgcccgca ttcgccgcac aaggctggtt tctttcacgc ccggcaccga tagaaacgct
    21481 gaatacggcg gttctggcgc tataagctgc ctcattttcc gcctggctgg actatcttta
    21541 ggactggcac aggaagaata tgaggaagcg aacgatgagc aaggcaggca aaataaccgc
    21601 tgcgatttca ggggctttct tgttgttgat tgtcgtggcg atcattttga ttgcaacttt
    21661 tgactggaat cgactcaaac cgaccatcaa ccagaaagtc tctgcggagt tgaatcgtcc
    21721 gttcgctatc cgtggcgatc tgggcgtggt gtgggagcgg caaaaacaag aaactggctg
    21781 gcgcagctgg gtgccgtggc cccatgtaca cgcggaagac atcattcttg gcaatccacc
    21841 ggatattccc gaagtcacaa tggtgcattt gccgcgagta gaagcgacgc tggccccgct
    21901 ggcgctgctg accaaaacgg tctggttgcc gtggatcaag ctcgaaaagc ccgacgcgcg
    21961 cctgattcgc ctctccgaaa agaacaataa ctggacgttt aatctcgcca acgatgacaa
    22021 caaagacgcg aatgcaaagc cgtcggcgtg gtcatttcag ctggataata ttcttttcga
    22081 tcaagggcgg atcgccattg atgacaaagt aagcaaagcg gatctggaaa tttttgtcga
    22141 tccgttaggc aagccgctgc cgttcagcga agttaccgga tcgaaaggta aagcggataa
    22201 agaaaaggtg ggcgattacg tttttggcct gaaggcgcag ggacgttata acggtgaacc
    22261 gctcactggt acgggaaaaa taggcggtat gctggcgctg cgtggcgaag gaacgccgtt
    22321 tccggtacag gctgatttcc gctctggtaa cacccgtgtt gcttttgatg gcgttgtgaa
    22381 tgacccaatg aagatggggg gtgtcgattt acggcttaaa ttttctggcg attcgctggg
    22441 tgatctctat gaactgacgg gcgttctgct gcccgataca ccgccgtttg aaacggatgg
    22501 tcggctggta gcgaaaatcg acactgaaaa atcgtcggtc tttgattatc gcggttttaa
    22561 tgggcgcatt ggcgatagcg atatccacgg ttctctgatc tacaccaccg gcaagccacg
    22621 accaaaactg gaaggtgatg tcgagtcgcg gcaattgcgg ctggcggacc tgggaccgtt
    22681 ggttggcgtt gattccggga aaggggcaga aaagtcgaaa cggtctgaac agaagaaggg
    22741 cgaaaaaagc gttcagcctg cgggtaaagt gctgccttat gaccgcttcg aaaccgataa
    22801 atgggacgtt atggatgccg atgttcgctt caaagggcgg cgcattgagc atggcagcag
    22861 cctgccgatt agcgatcttt ctactcatat catcctcaaa aatgctgacc tgcgcctgca
    22921 accgctgaaa tttggcatgg caggcggcag cattgcggcg aatattcatc tggaaggcga
    22981 taaaaagccg atgcaagggc gggcagatat tcaggcccgt cggctgaaac tgaaagaact
    23041 gatgcccgat gtagaactga tgcagaagac gctgggggaa atgaacggtg acgcggaact
    23101 acgcggtagc ggtaactcgg tggcggcgct tttaggcaac agtaatggca acctgaaact
    23161 gttgatgaat gacgggctgg tgagccgcaa cctgatggag attgtcgggc tgaatgttgg
    23221 taactacatt gtcggtgcga tatttggtga cgatgaggtg cgggtgaact gcgcggcagc
    23281 gaatctggat attgccaacg gtgtggcgcg tccgcaggtt ttcgcttttg ataccgagaa
    23341 tgcgttgatc aatgttaccg gcacggcaag ttttgcttcg gaacagctgg atttaactat
    23401 cgatccagaa agtaaaggga ttcgcattat cacgctgcgt tcgccgctgt atgtgcgtgg
    23461 gacgtttaaa aatccgcagg ctggggtgaa agccgggccg ttgattgccc gtggtgctgt
    23521 tgcggcggca ctggcaacgc tggttacgcc agcggcagca ttgctggcac tgatctcacc
    23581 ttccgaaggg gaggctaatc agtgcaggac aattttgtcg cagatgaaga agtgatgata
    23641 attgccggat gtctgacatc cggcaattaa aacgactaca acgactggtg ccgcgtctca
    23701 tgcgttaaca gcagggcaat taacgtcagt ccggccatcg ccgccagata taaccccacc
    23761 gcgcctaacc catagttagt ctgcaaccag gccgcgatat acggcgcaac agacgccccg
    23821 agaatcgacg atacgttata ggagaacgat gctccggtat aacggacttc cgtcgggaac
    23881 agctccggca gcagcgcccc catcggacca aaggtcaggc ccatcaaact caagcccagc
    23941 agcaggaaaa caaacaccaa tgctggatta ccagaaccca acagtgggtt aaaggcaaac
    24001 agggcaaaca gaatgataag cgtagtgatc accaccatgc ttttacggcg accaaaggca
    24061 tcagccagta atccggcgac gggcaccatc acgccaaaac caataactgc catcatcagc
    24121 atccacaaca cttcgttacg cggcaggcca agcccaactg gcgcggcggc ggtactaaag
    24181 gtcatagagt agaccgtcat gatgtaaaac agcgtatagg ttgccagcat aatgaacgta
    24241 cccagtacgg ttacgcgaac atgtttggtc agcagcgtac ccagtgggat cttcacctgt
    24301 tttttcgctt tagcgacttt ctcaaacacc ggcgactcat gcagcgacac gcgaacatac
    24361 aggccgataa tgaccagcac cgccgagaag ataaatggca cacgccagcc ccagctcata
    24421 aactgctcgt cggtcagtag ccaggaaagc agcaagaaag tgccgttggc aaagaagaag
    24481 ccaatcggtg cgcccagctg cgggaaggag ccgtacaatg cacgtttgcg cggtggggcg
    24541 ttttcagtcg ccagcagcgc cgcgccgccc cattcaccac ctaagcccag accctgacca
    24601 aatcgagcca gcgccagcag cagcggggcg aaaataccaa tcgtggcata gcctggcagc
    24661 agaccaatca ccacggtcga aatccccatc gttagcaacg aggcgaccag cgtcgcttta
    24721 cgcccaacgc gatcgccaaa atgaccaaaa acggcagagc caatggggcg cgcgacgaag
    24781 gcgatggcaa aggtggcgag cgactgtagc gttgctgccg ttggatcgcc ctgcgggaag
    24841 aagatatgcg gaaacacaat aacggccgca gtggcgtaaa tgtaaaagtc gaagaactca
    24901 atggccgtgc caatgagaga ggcgacaagg actttattac gcgagttgat cggcgtgtat
    24961 tcttgctcgt ggtcgagtgt tgtggctgtt gcttgcataa tcttttctta ttttttggct
    25021 aacgaatagc catattacgc acagcaaaag tgatatttca atgtgtaaca aatcgcggaa
    25081 atggtcaaaa atcaggcaaa aaggggataa tgtttaggcc agtgattttt cttctatgaa
    25141 atgcttcaca taattttata aatggtggat taaactggtt ttaggttaaa taaaagttac
    25201 caactggatt tatatgctgg ggttatgagt tgaaatgaaa ttgtttgctc ctgaatagat
    25261 ggctaaagag gctctgggaa agagtttcaa gaggttatgt gcgcattata gccggatgcg
    25321 atgctggcgc atcttatccg gcctacgggg agccaggccg aataaattcg tacgccgcat
    25381 ccggcctatt ttaaggctgc gttttccccg gcattcgcgg gtcgtcttta tattcggcgg
    25441 tggcaatcca cgccgcgcaa aacagcgtca aacgggcgaa gaagtagaaa aatgccatca
    25501 gccccagcac ggaaccaaat gctgcgccag agggtgattt catcaacgat ggcagggtgt
    25561 aggtcatcac gattttaatc acttcaaaac caatagcagc cagaaatgtt ccgcggatca
    25621 gggctttttt gcgcggacgg tggcgcggca accgccagaa gatccagaag aaaagcagat
    25681 agttggcgaa gatggaaatc gccaggccaa tcaaccgcca tgtcggttta agccactcaa
    25741 tgctgttcag gtgcagggcg ctaataatca tttgctgcgc agaaccggca accgaggtga
    25801 tagaaagcgt cacaatcagc gcaatcaaca aaccaatcag cgaaataaaa tcacgcagat
    25861 attttaccca taacttctcc tgatcttgcg gcgagcgttc ccagacatcg cgtgactggg
    25921 cgcgaatcgc ttcacgcagg ttacccatcc agttgatgcc ggaataaagc gccactgcca
    25981 gaccgacaag ccctacagtc gtacgctgct gaacggcggt gttgatggtg tttttcaacg
    26041 tggcggctag cgtcggatcg ctaatgtttt gcagaatttt gtcgaagata tcctgtagca
    26101 acatcggatg ggaggccagc acaaagcccc ctgcggcaaa cgacaccatc aaaatgggga
    26161 tcatcgacaa aaatgagaaa taggtgatag ccgccccaaa ctggttaccc agccgatcgt
    26221 taaagcgttc tgtcgcgcga atcaggtgcg caataaccgg ttggcgttgg actttctcgg
    26281 cagtggtggt gacggtttcc agcgcctggc taactttgcc gcctttctcg ctattatcca
    26341 actgcttaat cggctcgtgc tccagatcct gggtgggacg tttgatctcg ttttcctgcg
    26401 tcatccgctc ttttgtcctt ctgtcgtggt tattcttaaa aagtatagtc ggtcagtcca
    26461 ggtgctcttt cattacgcca gccagccact gcataaacag gtttacacgc cgggaaagct
    26521 cccgacgctg cggataaacc agtgaaaggg acagcggctc ggcacggtag ccaggcaaca
    26581 cttcaataag ccgcccggca cgcagggctt cgcgcacggc aatgcgcggg atctgaataa
    26641 tccccagacc ggcaataccg gcggcgagat agttttcgct actgtttacc gtcaacatgc
    26701 cgccagactt aaaccactgg acgccattaa cgctggcaac ctcaaaacct aacggatgta
    26761 cacccaggtg cggtgtataa cgcactattg cgtgtgaagt cagatcatcg gggctttgcg
    26821 gataaccaaa gcgcgtcaga tagtgtggac tggcacagtt gaccatggtc agtttgccga
    26881 gagaacgggc gataacgcca tcttccggta atgcgccagt gcgtatcacg caatcaaaac
    26941 catcatgaag aatatctacc ggacggtcat ggctactcag ttccagctca attcccggat
    27001 gcagatagag aaattccgac aagcgcggca gtaacaggct tttcgcgatt cctgacggga
    27061 tgtcgatgcg taatttaccg ctgatactgg tggcatcctg ttgaaacaga ccgtccagtt
    27121 cgctgagatt actcaacaca tcttttgccc gttgataata ggtcatgcct tctggcgtga
    27181 gtttgacccg tcgcgtggtg cgctggagaa gctgggtgcc aagttgatgt tccagtgcct
    27241 gtatctggcg cgaaacactt ccctttggca aagcaaagaa atccgccgcg cgggaaaaac
    27301 tttccagctc cgcgactttg atgaacaact gcattgcgtg aattttatcc atttctttga
    27361 ccgattgttg ttcacaacga aacagtagcg cgcatatcgc attgtttatt gttttttcta
    27421 acatctaatg ccgggtttca caatgttgtc atgatgaatg tggcgatttc tcagcaggaa
    27481 aagggaggaa tagctttatg agaaaggtga attcccttaa acagagcgta tttttcagcg
    27541 attcccttta aagtgaatag aagatgtatt ggctatttgt gcttattttt atctcattgc
    27601 cgtttgttat ttttgtttat ggtcgcggaa tttcattttt aattgcatgc gtactaacta
    27661 ccgatataag gaaatcctca aaaatgtttt aaatagtgcc ctcgacatgt aatttcaggc
    27721 gtttagctgt aactatttat atcggtaact attttctacg cgtcatctct gtagaatggc
    27781 caccgccaaa tggcgaattc ttattttttc tcgcgggtaa tacccttcca ctacttaagt
    27841 ctttttaacg tattcaggtg gagattcagt tatgcaaata gtcatgtttg acaggcagtc
    27901 aatatttatt catggaatga aaatcagttt acagcatcgt attccaggag tgagtattca
    27961 gggggccagt caggcagacg agttatggca aaagctggaa agttaccctg aagccttagt
    28021 gatgctcgat ggcgatcagg atggtgagtt ttgctacagg ttgctgcaaa aaaccgtggt
    28081 gcaatttcct gaggttaagg tgttaattac ggcgacggat tgcaacaaac ggtggttaca
    28141 ggaagttatt cattttaatg tgctggccat tgtgcctcgt gattcaaccg tcgagacatt
    28201 tgcgttggcg gtaaatagtg cggcgatggg gatgatgttt ctgcctggag actggcgaac
    28261 gacgccggaa aaggacatta aagatctcaa atcgttaagc gcccgccagc gggagatttt
    28321 aaccatgtta gcggcgggcg aatcaaataa agagatcggc agggcgctga atatcagtac
    28381 cggaacggta aaagcgcatc tggaatcact gtatcgccgt ctggaagtca aaaatcgcac
    28441 ccaggcggca atgatgttaa atatctcctc ctgaagagga taattttaca gccagcagga
    28501 agttagcggc tggctgtaaa aatattatgg ttcgccgtac aaaccaatta aacggcgtac
    28561 cacaccgtta gtccagccaa acccatcctg caacggatac tcgccaccgc cgccttcgcg
    28621 aggaacacca tcggcaatat ggtatttctc gatcagtttg tgctgttcca gatagaactg
    28681 attcaccgtc ttcagccagt tgcgcgcgat ttcatcaccc agaaggtcat cgccgtacat
    28741 cttaaatccc tgaattgcca tccattgtaa cggtgcccag ccgttgggtt tatcccactg
    28801 ttcaccggtt tcgtactcgc ttgccagaat cccgccaggt gtcagtaacc ggctgcgcac
    28861 ggcgtttgcc agacgatcgg cctgttcatg gttcgccata ccgacataga gcggcacaat
    28921 ggcggcagcg gaaaacagcg ccagttgctc gcgtcgccag tcgtaatcgc ggtagatgcc
    28981 gttttcatca tcccagaggt aacggtttac cgcatcgcga cgggcactgg ccttctggcg
    29041 gaacagtgct tctgtctctt tctcgccttt cagcgccgag atgttggcga tggcgctctc
    29101 cagtttaaac aggaaggcat tcagatcgat ggggatgaac tgggtggtac gaatgctcgc
    29161 cagacgacca gtatcacgca gccaacggga agagtaatcc caaccggagg ccgcccccgc
    29221 acgtaaatcg cggtacacct cgttaggcgg gcgaccagaa tgtttcgcgg tttcaacgtc
    29281 ctcaagccag gattcgtcac gcggcgtgtc gcgatcgtcc cagtaacggt tgagcagcga
    29341 gccgtccggc atccgcacaa catggcgata ggcctgatta gggatcaacg attctgcacc
    29401 gtccatccag aaggcatatt ccattttcag gtggtcgaga tagcggcgcg cgccgcgtac
    29461 accatcttct tcaaacaact ccaccatcag cgcaaaaacc ggtggttgcg agcggctcaa
    29521 ataataggtg cggttgccgt tggggatgtg gccgtagttt tcgatcatcc aggcgaagtt
    29581 atcggccatg catttcagca aatcttcccg accactttcc gccagcccca gcatggtgaa
    29641 ataggaatcc caatagtagg tttcgctaaa acggccgccc gggacaatat atgactgcgg
    29701 cagcgccagc agagaagacc acggaatgtg atcctgtggt tcacgggtta gcaccggcca
    29761 cagctggtcg atatgctctt tcagggaatt ttgcgggtcc gatacatact cgctggagta
    29821 gacctccggc agccagaagt ggttttcaac aaacttgcgc aagtcaaaat cacgatgacg
    29881 gcgcacttta cggtagcgga ttaaaatatc cagcgggtcc attttgggtg cgcagtcggg
    29941 aaaggtttta ctgtcgggga aaattttcgc cgactgaaca tgctcgaaca gttcgagata
    30001 gcgatcggca ggcgtcagcg catcagaggc gggcagccct tcaatcatct cgggttccgg
    30061 ttctgcctcg atcatctcat ccagttttaa ttcatacggg tccagctcat agcagagatc
    30121 gacctcgatc atcagttcgt ctggattagg gttttgaatt ttctgattga gcataaggat
    30181 ccgacctccg aaattcgtcg ctaattatcg ggcttttctt taagggtagt cattcgctcc
    30241 gcctggtggg cgacaaagcg tgcggtggat cacgtttatt aacctgccaa aaaatataac
    30301 gcactaatat caatggaatt tataaaaata cggatttcgt gttgtttttc attttcagaa
    30361 cattcaatcc tgctctcgcc gcaatcatga tatgccattc ctgatctggg taataggcac
    30421 tggctatctt attgatagtt tatattcatg taattgattg attcctatca aaaagcatat
    30481 ctccctgttt tttatgatgg ctttgtctcg cagagacgtt tgcagcattc tggaaaattt
    30541 tctgggtcac aataactcac taccgtgagg gaagaataat gaaaatggtc tcacgtatta
    30601 ccgcgatcgg cctggctggc gtcgcgattt gctatttagg gttatctggt tatgtgtggt
    30661 accacgataa taaacgcagt aaacaggccg atgttcaggc atctgctgtc agtgaaaata
    30721 ataaggtttt aggctttctc cgcgaaaaag gatgcgatta ttgccatacg ccttcggcag
    30781 aattacctgc ctattattat attcccggcg cgaaacagtt gatggattac gacataaagc
    30841 ttggatataa atcttttaac ctcgaggccg tgcgtgcggc tctgctggct aataaacccg
    30901 tttcgcaaag cgatctgaat aagattgaat gggtgatgca gtatgaaact atgccgccaa
    30961 cgcgttatac cgcgctacac tgggcgggta aggtgagtga tgaagagcgg gcggaaatac
    31021 tggcctggat tgcaaaacag cgcgcggaat attacgccag caatgatact gctccggagc
    31081 atcgcaatga accggtgcag cccatcccgc aaaaactgcc taccgatgcg caaaaagtgg
    31141 cgttgggctt tgcgctgtat cacgatcctc gtttatcggc tgatagcacc atttcatgcg
    31201 ctcattgcca tgcgttgaat gcgggaggcg tcgatggtag aaaaacatcg attggtgttg
    31261 gtggcgcagt tgggccgatt aacgcgccga cggtatttaa ctcagtattt aacgttgagc
    31321 agttctggga tggtcgtgcg gcaacattgc aggatcaggc tggtggaccg ccgttgaacc
    31381 cgattgaaat ggcgtcgaaa tcctgggacg aaattattgc taagctggaa aaagatccgc
    31441 agcttaaagc gcagttcctc gaagtctatc cgcaaggttt cagtggcgaa aatattactg
    31501 atgccattgc tgaatttgag aaaacattaa ttacgccgga ttccccattt gataaatggt
    31561 tgcgtggaga tgagaatgct ctgacggcgc aacagaaaaa aggctatcaa ttatttaaag
    31621 ataataaatg tgcaacttgt catagtggta ttattctcgg tggacgttcc tttgaaccgt
    31681 tggggctgaa aaaagacttt aactttggtg aaattacggc ggcggatatt ggtcgtatga
    31741 atgttactaa agaagagcgt gataaattgc gtcagaaagt acccggttta cgtaacgttg
    31801 ctttaacggc accgtacttc catcgcggtg acgtgccgac gctggacggg gcggtgaaac
    31861 tgatgctgcg ctatcaggta ggcaaagagc tgccgcagga agatgtggat gatatcgtag
    31921 ctttcctgca cagtctgaac ggggtttaca cgccgtatat gcaggataaa caataattaa
    31981 tttgatcgcc cgaacaggag tgtttgggcg atttttatta cgataataaa gtctgttttt
    32041 aatattat
//
LOCUS       DADSYM010000040        31911 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2415
            SAMN08039579-rid11723173.denovo.040, whole genome shotgun sequence.
ACCESSION   DADSYM010000040 DADSYM010000000
VERSION     DADSYM010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039579
            Sequence Read Archive: SRR6362533
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 31911)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31911)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980162.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 03:39:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,484
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 4,347
            CDSs (with protein)               :: 4,347
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 43 of 85
            Pseudo Genes (incomplete)         :: 37 of 85
            Pseudo Genes (internal stop)      :: 19 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31911
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039579-rid11723173.denovo.040"
                     /strain="LGE2415"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8302 N 95.9628 W"
                     /collection_date="2015-08-24"
     gene            1187..1903
                     /gene="nanC"
                     /locus_tag="J1Y83_001557"
     CDS             1187..1903
                     /gene="nanC"
                     /locus_tag="J1Y83_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313297.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminic acid outer membrane channel
                     NanC"
                     /protein_id="HBA7629714.1"
                     /translation="MKKAKILSGVLLLCFSSPLISQAATLDVRGGYRSGSHAYETRLK
                     VSEGWQNGWWASMESNTWNTIHDNKKENAALNDVQVEVNYAIKLDDQWTVRPGMLTHF
                     SSNGTRYGPYVKLSWDATKDLNFGIRYRYDWKAYRQQDLSGDMSRDNVHRWDGYVTYH
                     INSDFTFAWQTTLYSKQNDYRYANHKKWATENAFVLQYHMTPDITPYIEYDYLDRQGV
                     YNGRDNLSENSYRIGVSFKL"
     gene            1923..3029
                     /locus_tag="J1Y83_001558"
     CDS             1923..3029
                     /locus_tag="J1Y83_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418730.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate epimerase"
                     /protein_id="HBA7629715.1"
                     /translation="MNKTITALAILMASFAANASVLPETPVPFKSGTGVIDNDTVYIG
                     LGSAGTAWYKLDTQAKDKKWTALAAFPGGPRDQATSAFIDGNLYVFGGIGKNSEGLTQ
                     VFNDVHKYNPKTNSWVKLMSHAPMGMAGHVTFVHNGKAYVTGGVNQNIFNGYFEDLNE
                     AGKDSTTIDKINAHYFDKKAEDYFFNKFLLSFDPSTQQWSYAGESPWYGTAGAAVVNK
                     GDKTWLINGEAKPGLRTDAVFELDFTGNNLKWNKLAPVASPDGVAGGFAGMSNDSLIF
                     AGGAGFKGSRENYQNGKNYAHEGLKKSYSADIHLWHNGKWDKSGELSQGRAYGVSLPW
                     NNSLLIIGGETAGGKAVTDSVLISVKDNKVTVQN"
     gene            3094..4074
                     /locus_tag="J1Y83_001559"
     CDS             3094..4074
                     /locus_tag="J1Y83_001559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialate O-acetylesterase"
                     /protein_id="HBA7629716.1"
                     /translation="MNAIISPDYYYVLTVAGQSNAMAYGEGLPLPDGEDAPHPRIKQL
                     ARFAHTHPGGPSCHFNNIIPLTHCPHDVQDMQGYHHPLATNHQTQYGTVGQALHIARK
                     LLPFIPDNAGVLIVPCCRGGSAFTAGSEGTYSERHGASHDACRWGMDTPLYQDLVSRT
                     RVALAKNPQNKFLGVCWMQGEFDLMTSDYASHPQHFNHMVEAFRRDLKQYHSQLDNIT
                     DAPWFCGDTTWYWKENFPNAYEAIYGNYQNNVLANIIFVDFQQQGERGLTNAPDEDPD
                     DLSTGYYGSAYRSPENWTTALRSSHFSAAARRGIISDRFVEAILQFWRER"
     gene            complement(4082..4204)
                     /locus_tag="J1Y83_001560"
     CDS             complement(4082..4204)
                     /locus_tag="J1Y83_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012139841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HBA7629717.1"
                     /translation="MISFVFLLPVYPNCHAMLHRRKPPFTPEELKALMDANKSN"
     gene            4286..5158
                     /locus_tag="J1Y83_001561"
     CDS             4286..5158
                     /locus_tag="J1Y83_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004186812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HBA7629718.1"
                     /translation="MASSKSLQQAIANIKIWHKGEQRAPHKPLLLLYVLAGYLNGHPR
                     LFDYGSEIYEPLHSLLERFGPQRSQYRPDMPFWRLQGDGFWQLHNAGLCSTAGSSRQP
                     PVKELTEYHVAGGFDEQHYALVTGNKKLINTLAQQILEAHFTESIQEEIADELGFDLQ
                     QIRKQRDPLFRKNVLRAYNYQCAICGFNMRHDDTTVALEAAHIKWKQHGGPCEIPNGL
                     ALCAIHHKAFDKGSIGLDENMRVLVSDAVNGGGIVERLFWDFDGKTIALPQVRKNYPY
                     EGFVEWHRKEVFRG"
     gene            5328..7403
                     /locus_tag="J1Y83_001562"
     CDS             5328..7403
                     /locus_tag="J1Y83_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000177022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA domain-containing protein"
                     /protein_id="HBA7629719.1"
                     /translation="MTLVDSVEAGRLTISELIDALAKDKNYTASRWYQRYRAFTTLLQ
                     QTSTFAEPATDGLVKQLWYERDNGIASIRQGVPSLAEYQQSLPLLRELTERIRQQPDE
                     ETYQYVGNALKQAKENGLLKRMYWSLRNRVFAAFSPENYTSTVDENAFNKAAEFLNQH
                     FHLGLVLTGNWLQKNYEVKQAIHARSPDTDPYYVNMAIWHLYELLRERDNEQKQEKVA
                     STTSITSSEPIENKIILHSPTNVIFFGPPGTGKTFRLQQKMKEYTSHAVPADRDAWLD
                     SRLESLNWMQVITLALLDLGKRAKVRQIIEHMWFQRKALLNGRNGNLSNTAWAALQSY
                     TVPESLTVDYKNRREPAVFNKTDNSEWLLVDSQLEQVEDLVELYAELKRGPKSAEAIQ
                     RFAVVTFHQSYGYEEFIEGIRARSDESGNISYPIEPGIFMRLCQRANADPGHRYAIFI
                     DEINRGNISKIFGELISLIEVDKRAGMPNAMSLQLAYSGDHFSVPGNVDIIGAMNTAD
                     RSLALMDTALRRRFDFVEMMPDLSLLSGAKVKGIELESLLEKLNSRIEALYDREHTLG
                     HAFFMPVKNALDAGDEEAAFKQLKIAFQKKIIPLLQEYFFDDWNKIRLVLADNQKQDD
                     NLQFVIEKTDDLDTLFGNNHGLRRHDQQSTAYELKDFDQEIWNIPQAYRSIYQPQQTP
                     LDEQAVNHG"
     gene            7396..8739
                     /locus_tag="J1Y83_001563"
     CDS             7396..8739
                     /locus_tag="J1Y83_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001617353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="McrC family protein"
                     /protein_id="HBA7629720.1"
                     /translation="MGEVISVFEYDLLGSGKAASVGAKPVPPQVFNYLEALSLASNQG
                     SQFLKLTSRSGFKLLQVQNYAGMLSTPHGFQLEILPKVGKNLTAANARQTLLTMLSHL
                     PGFRHIQTQQATLQAQRMPLLEIFINQFLHSVSQLLKQGLRSDYVSKQGNLAFMKGKL
                     MLSAQLRHNAVNRHKFCVDYDDYMPDCAANRLLHSALDKLLSLKLSSENQRWLYELRF
                     AFDGIPLSRDIERDINNLRLERGMAHYNEPMAWAQLILRGMSPSALQGNTKAISLLFP
                     MEAVFESFVAQTLPYELPSHLKVLPQAATYSLVKHGVKDCFKLRPDLLIQSNKPVQTE
                     MVMDTKWKLVNSSQQTKSLYGLAQADFYQMFAYGQKYLGGNGEMYLIYPAHDDFSQPI
                     PQDFAFSETLKLWVVPYRIMAKRGERMMWPNDVSCTMSPKLDWMAASVSYSGGMR"
     gene            8736..9122
                     /locus_tag="J1Y83_001564"
     CDS             8736..9122
                     /locus_tag="J1Y83_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004222267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA7629721.1"
                     /translation="MTHEYQRQSLEQLITQQTATPRSAAVVFNENQTLWQTLGFSQSQ
                     VSLWLASLPQSNHLKGPTTATYQVIPDIAAHLVSLLHQAGGRMPLAQVLKKLPAGITT
                     SEQQIRKLAQKHAQIEIKGPLLVLIN"
     gene            9173..11320
                     /locus_tag="J1Y83_001565"
     CDS             9173..11320
                     /locus_tag="J1Y83_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001040176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HBA7629722.1"
                     /translation="MAKEAKKQDPQQQLEALFNKLVKQHSEFTQPLVELPEHEFAKAG
                     LIQGLVEGNLTPPVMLIALTEGHWEYAADTAKFDDLAYQLLSDIEGDWPVYAVVDDGL
                     NQRILALFGADGSDGTHGADTLPGLEELRSFDRMERDPTFRWSMRVYTRLMQRFDAFH
                     ENVYRVTKDKVNDKNDIIEEVAKLLFLETFRLHHDEDLTFKDDEGNTLRFRDVFDWQY
                     VESHGDKAVAQIKAAFEEFKNHENYVVISDDGSRNPIFSKETHLRLSVAKNYQDLLEA
                     IQNLGPVKTNDGKIAKEHGTLADVSGDLLGRVFDVFLRANFESKGGLGVYLTPNPVKQ
                     AMLEIAMHDIDDDDEMRSRLANGDFRFCDPTCGSFGFGSVALSQIDKWIDFKLVLADD
                     KKESLKQKLRDCAFTGADAAPRMVMLARVNMALQGAPKAQIFYTDNSLTTNALKPNSF
                     DLICTNPPFGTPKFDKGKNGQNSKANYEANMEQVLGGFRPTKKVVDSYNAWYDHVKMK
                     WQDIGDLELDENGEPKWAGYRTDLRRTGGTDKKPFYSLQPTTAGLALGSKPDSKGNWQ
                     PVGATIDPAVLFIDRCLQLLKPGGRLLIVLPDGILCNSGDRYVREYIMGKKDEKTGEF
                     VGGKAIVKAVISLPSDCFKLSGTGAKTSILYLQKRHANPNQPEQFLPEPQTDVFMAVA
                     ETLGYVVKNNIEDYNAGVANDLDKIVSAYKRGV"
     gene            11320..11958
                     /locus_tag="J1Y83_001566"
                     /pseudo
     CDS             11320..11958
                     /locus_tag="J1Y83_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003924711.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
     gene            12607..12855
                     /locus_tag="J1Y83_001567"
                     /pseudo
     CDS             12607..12855
                     /locus_tag="J1Y83_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000190890.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
     gene            12873..14159
                     /locus_tag="J1Y83_001568"
     CDS             12873..14159
                     /locus_tag="J1Y83_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019106739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA7629723.1"
                     /translation="MDSSTTRQHNDPVNSEAALNLCLQLWQQGGLNANKAALLLAAVP
                     ALRSLLQPIILPQKNDAETDIVSAFSLTAPLLDAFNDLSQSGEWQLALLGLNPDVRQH
                     WINLAAARCQEAGAMNDIMVLVKLIQQLGNASEWVLAQLESTATTPQIIAGPLAKTER
                     DLLGHSLNDNAAIPALCRILHTSHTLFTVSEQNEPPAPIQAVDVTAKQLTNNWCSGRL
                     LALPNTLLDEHDLKPNADWLLVSRSGHDNVPLTELFAQQPWLFLLSLIIFVQDAWAAE
                     QRGGLLLTLPAGQNAFAPGQINVAVQGIEGDEVSLGSLAEFLVLLLGELNIPLYPALD
                     ANTESINRLNRVLSSFIAELLAKKIWQFTEAGRGESGQYRIHTSFSDACYSLPLAPLF
                     GYKSQTLQRAIKQLAQNCYANKKRAANRINLQGSSL"
     gene            14156..18442
                     /locus_tag="J1Y83_001569"
     CDS             14156..18442
                     /locus_tag="J1Y83_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001584427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA7629724.1"
                     /translation="MSFELNEQMVDSLAKQHRWAATGVSDYPPRDPLVGQSRFFKRFQ
                     TFLHTVDHDDDRFAHVFAVEAEWGRGKSRLGHELVAQINDCSRGWFVRDEQGQLHDKQ
                     LFNRDTQDKYLALYIRYSQVASDYQNSDNWFAFGLYQALLPLATQTFDGSIQSEIAKQ
                     AYERLMPFGFKSELLADALQLNANHSEEDLYTDQTLVVSLVQAAYKVLKSFGVQYMLV
                     VLDELETVAEAATFGLEDEQDKRLDGQAIRLIGKAIKEEDPRRKLPWLRYVALCSPLL
                     GQQLREIQSVARRFELVELEHNAFADVSDYVASLLKDGKLGFNYPAGLVEAAYAMSGA
                     NFGWFNVVMANVDAVLSQYQQAGRSIKDVGDLFEAVLAGSGRVARHVLDKGAIEGIQT
                     RDQALLTECRTLLYGQLPVRLTEVSHATELLALKNEDLEPIAAHYRKLSIDRLQCRQA
                     LEQAKFRRDKDEWYYPAVDQALNLDTLLDNLRTFSIKEQAASGDKGAVLLPLSKGEFK
                     YLLSLLYDHPAIEFAADALWHKLVGETTELDASEATHIGPSVAMLLRLDLRYRSAQQN
                     SLIFKDPAMGDAHEQAMSELSKQSSQKPLIKLLARVTGFVRLLDNNWTYDENLLSNKA
                     GSDDAPLAILTEPRGRQGGLLTLDGFKLHPEGKALFAWVSNLIELNNLHTFAANHSHQ
                     NGRTPVLALTASAHLMEQYSRLDERNELRDAILLHYVNPSEADQLERIGLSLAACQLH
                     GVNLTADSFTAKFKNKLHALTTFATDAIHKWRQRLQQRGLIAWPLKVDGKLSSNDRDL
                     LFKGWYQLAIAHPELNGILDLQQQHGVPVNELSSLLDKLKVPGSYIAKGYTADEHAGL
                     FTELNNVQRSQAQIPLFLARIAHPNKKHKWQFEGFKQQFYFAYVAETSVTAKGVFNDW
                     MWWCGELNLLTLTNPTEKQAIWEHYPRSRLENAIREAQNWFRGNDMGSYAANVEVMSR
                     VYGYARINEMFAPLGKNKLGFVTVEAKEQLEKAQSLFNVLKQQEEQLADMVEANDTKV
                     LAGLIHKRAEVLELVAKVKPLNSSRPMLKDAHILSLEDKTTSLYQRIEQACLFAEFVE
                     YSAERINNRLVDLIIDVETECAPLTNFPKRLYTNTLRTIGHILDGALKDDTSSATGRK
                     EQQADSDTLLHYLRKLDLGRAHDKLSALAQEVGLNLHNDQQLPIAEIQGHILSSYRNC
                     KERFSKLVNNLTEQKLRAQQLQEWLSSATAEYQFTDDIAELPKLVMKLQLIEDAIADL
                     PNDAESKRQSMQNSLRNGQFSSLRDLPEELLKPARGQLTPIQGQLLKIEERLNQVRRN
                     SIAQVNSWLPLLKPLLASQKQAEPAALTLEDVSNLGVRELQQVCESTQAKWQSQGEQI
                     LNDTGLTLADWQPIYQALSQNQEPVLTPEQQKGLVDKGIVKMRLTFATGL"
     gene            18453..19301
                     /locus_tag="J1Y83_001570"
     CDS             18453..19301
                     /locus_tag="J1Y83_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004222254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA7629725.1"
                     /translation="MSLYLSAALASNRKGRFLQTVAGATPLTKDWISSPPASGLLIMQ
                     AEELTDANTMQHLYHWAMQAGCAALVINLKAEQFTLLAQLPYPLDWQLVPASLRRQEP
                     GLTALLASETDQAIAGFTGSADRYQHQAGDVVHTRYIRKHSNSGLLAFTTLPLWSLTL
                     LDHSELLVSWLNWFVDHAGIAERIIEPKAPSTDYTPDKHDLVVLLLLYAGGGMNLQAL
                     SEHNAVKLMFDVNSLDIVKRGEMLRQHDFIDDAGITATGKTCLQASQYWAYAPLLGEQ
                     LHTGTL"
     gene            19304..20461
                     /locus_tag="J1Y83_001571"
     CDS             19304..20461
                     /locus_tag="J1Y83_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001004905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA7629726.1"
                     /translation="MNENKTMNLAQSLAIDSQLRLPGSAGRLLKQVRDIEQVAPLFRD
                     AGLVKAVPKPTIVNLRVAGIATKSCLDKVLGGFIYASAAVRTDLLIDDNKVSTVGSDS
                     VSELDDIDFEAQRKRLDLIEIRQAYQLVEKKLLSYEPGDLILLDTPLFLARDMAPLER
                     NVRHKQEYEKTRQVIETFWQNHRSRIFPWNQNGVVLASILAERFSAIVSIAKQDLRTE
                     QGRKQILASDGFSKELMQNLEGLDESLVGVGDLRFINGILGNYSRTIAFRLSEQEKRI
                     EPNIEAKQGLIGFHFRSARGGQIKMVQLAGDEPEWKSADLDLVASRLMVLDMQNKGNA
                     MPLPQLLGYQQLGILPKFATFYRQGLHQALKNNDIEAGWLAGLDEGLNNGL"
     gene            20451..22136
                     /locus_tag="J1Y83_001572"
     CDS             20451..22136
                     /locus_tag="J1Y83_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019106735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBA7629727.1"
                     /translation="MAYKVLGKLVGNTGDASQLTMVVQDSFSVRRGEFVRIMHQERKD
                     EVQVAVLGRVTKISRTNMLYNAGFGDGVTELELLPGANVTGENMFAQVELVGYRDPVT
                     RQIKIPRRPLNPGTAVETVDFQFLSDFYEFNEHSSLHLGNLVGYDKGENTVPVFIDVN
                     KLVTEHLAVLAMTGSGKSYTVGRIIERLVAVNNGTVVVFDPHGEYGKALAGGNLQFSS
                     FLEATDDKRDQEALPLIKQTFEKLQTAGAGIQVYSPQHESFKHKYASKNTPLALQFDH
                     FEMDDIAEILPGLTEPQQRVLDVAIRYWRSADKTEPRDINRLRFLLGDGIEELKEWDD
                     LSEAEAKALSGRSAAVASMKLSRVLNEAKSFYSATMAEPTDIYKMVGRPSNQQGRLVV
                     VDLQGLSDTAKQVICALLSSEILKAASSKTDPLRPCFIIYEEGHNFAPAGGNAVSHRI
                     IKKIAGEGRKFGVGFGIVSQRPSKLDSDVTSQCNTLITMRLKNPDDQRFIAKASDMVS
                     KADLEELPSLSTGEALVCGRSIPAPLLVKVGSKALIHGGESPEVLRVWGSFSG"
     gene            22273..22686
                     /locus_tag="J1Y83_001573"
     CDS             22273..22686
                     /locus_tag="J1Y83_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001368082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA7629728.1"
                     /translation="MAVVECPAPRTFGADIRSDSGWFHKSSASPVCLIEFERFDGSAK
                     GQQKLEEKLKNLLEAAQRWNHCPKTLVLSAWSQGLVGVPDTQKLKDICRMGFTSSTGT
                     QVIAAPDVEVVFSRFLFIKNLNMIVLDRIHYEVLM"
     gene            22683..24152
                     /locus_tag="J1Y83_001574"
     CDS             22683..24152
                     /locus_tag="J1Y83_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001022617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein kinase"
                     /protein_id="HBA7629729.1"
                     /translation="MNIKSAHFLPGTNKRLGDKYLLLECLGDGSHGWVWRAERLKDGK
                     IVAVKIPKDITREDRQLAEGKELLNVEPHENIVQIFDMGRIDNEWFYIEMEYFPSQTL
                     AQKLDDRQRNFGQTYERLFRIYRQVLCAVHYLAELPVPISHGDIKPHNILVGERDLVK
                     LTDFGSSALPEEIYVRTRENGGTVLYSAPEFSNVDSRRGSLEELLLGDIYSLGVLLYQ
                     LLTGKLPHDTPAQVQRHAPFKLPTEINSSIHTDLEQVVLTCLQKRAEDRFSTISDLIH
                     AFDSAATKQLKVGAIAPVFQTKPAQDWSSAVLEAMSDQSYQKAAQLASQEYSRSKDLQ
                     ALHQQLIALYRANRLFDFEKVVEDNKAILLEGKLTQPAALFELIVKANLQLRNISQAN
                     FWLLQRKQAEAENAATMYLESSILGLEAKYSEARVLLEKVNQTTPMKFHVLSQLILVC
                     EQMRDYSGAAAYLKAALRVAPLDNSMKEKKELYAKLGVI"
     gene            complement(24352..25617)
                     /locus_tag="J1Y83_001575"
     CDS             complement(24352..25617)
                     /locus_tag="J1Y83_001575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019106732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase arm-type DNA-binding domain-containing
                     protein"
                     /protein_id="HBA7629730.1"
                     /translation="MALSDVKIRSAKPEAKAYKLTDGEGMVLLVHPNGSKYWRLRYRF
                     GGKEKMLALGKYPEVSLVDARGRRDEARKLLANGVDPSENKKAVKVEHEQEVITFEVV
                     AREWHASNQKWSASHSARVLKSLEDNLFAAIGKRNIADLKTRDLLVPIKAVESSGRLE
                     VAARLQQHTTAIIRFAVQSGLIDYNPAQEIAGAVATAKKQHRAALELNRIPELLQRIY
                     HYSGRPLTRLAVELTLLVFIRSSELRFARWSEVDFDTAMWTIPGEREPLEGVKHSQRG
                     SKMRTPHLVPLSRQALTILEKIKSMSGNRELIFVGDHDPRKPMSENTLNKALRVMGYD
                     TKTEVCGHGFRTMACSSLIESGLWSRDAVERQMSHQERSSVRAAYIHKAEHLGERMLM
                     LQWWADYLDANRMAAITPFDYAKINSSFR"
     gene            complement(25947..26744)
                     /locus_tag="J1Y83_001576"
     CDS             complement(25947..26744)
                     /locus_tag="J1Y83_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014837283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBA7629731.1"
                     /translation="MLQNNIKQLRTQLSITQRELAFMVGTSQQQIQRIETGKVAAKLG
                     LAQAICNALDKPLNVVFPESDRLINDFRKKRRKTDEDLEAIATSGIEMDSGLWTVKIW
                     LQGQRDYLLLPISAADKRRFYYYFQEKNTPNIERFFVFDSSEYRYALNMREVVFHQFL
                     SDGLRPIVDEEDDAYEDDYFNVHITLVNGGPVIPLSVEPDAPQNEETDDIGQLNAFFD
                     ALDCEPETTDRFMITDEDGEDAFIRIGSIAMVRVALDALEPVEDDEE"
     gene            complement(26858..27133)
                     /locus_tag="J1Y83_001577"
     CDS             complement(26858..27133)
                     /locus_tag="J1Y83_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012015892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HBA7629732.1"
                     /translation="MEIYWTLKAQDDLERIYRFALQYSRQHADDVLDRLITGCAGLTA
                     HPAIGVQQTRYEPREVRKVLFDDYEVHYELRDNDIYIVDLWHTREER"
     gene            complement(27137..27385)
                     /locus_tag="J1Y83_001578"
     CDS             complement(27137..27385)
                     /locus_tag="J1Y83_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004155191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix protein, CopG family"
                     /protein_id="HBA7629733.1"
                     /translation="MGSLTKQTISAQIPIELAAAVENLAIELDRSKSWIIKEALTSML
                     AERERRHQSIQAGLADVDAGRVMSHSDMVDFANRLKKS"
     gene            complement(27462..28295)
                     /locus_tag="J1Y83_001579"
     CDS             complement(27462..28295)
                     /locus_tag="J1Y83_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014227719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4942 domain-containing protein"
                     /protein_id="HBA7629734.1"
                     /translation="MQTEAEVLTDHNELICSTSIERIVTGRDSALNQIATLIQKLHDI
                     SSLTSSIGGDVAGTWAMRNGYAFDCWLMQPTDKAMPAITRNIDRSIWRDLMLKSGMLT
                     LMDAEARSQWAKNLEEGDLPAISEANILSTFEQLHHNKQDVFERGIINVFKGLSWDYK
                     TNNPCYFGKKIIVNNLVKHDRWGYSLNWGWRRDQLADLERMFYLLDGKAIPDNRHDVS
                     IRFMDFVRDNPHQQIFEDELFTIRYFQKGSGHITFKRLDLVDKMNDIVAKHFPGMLPA
                     K"
     gene            complement(28410..28751)
                     /locus_tag="J1Y83_001580"
     CDS             complement(28410..28751)
                     /locus_tag="J1Y83_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001683515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin"
                     /protein_id="HBA7629735.1"
                     /translation="MKIIPATTLRATTSYLSPVVVWQTLLARLLEQHYGLNLNDTPFN
                     NEKVIQEHIDAGITLVDAVNFLVEKYELIRIDRKGFSWQEQTPYLRAVDILRARQATG
                     LLRRCHNTTAR"
     gene            complement(28782..29117)
                     /locus_tag="J1Y83_001581"
     CDS             complement(28782..29117)
                     /locus_tag="J1Y83_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000939440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system YeeU family
                     antitoxin"
                     /protein_id="HBA7629736.1"
                     /translation="MLQKDKVMQQEWGLPCTVTPRFTARLVQERHNLHYLADRAAITG
                     KFSSPDLLHIDQAFPVLMKQLELKLADGELSPCQQRTVTLYAKGLTCEADTLGCCGYV
                     YIAIYPTQR"
     gene            complement(29117..29590)
                     /gene="radC"
                     /locus_tag="J1Y83_001582"
     CDS             complement(29117..29590)
                     /gene="radC"
                     /locus_tag="J1Y83_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001292742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HBA7629737.1"
                     /translation="MSELMLTPVFSVSEQRVIRRAMRLLEKYQRNPGESFLSSDATKT
                     WLQLHLARQEREMFVVLYLDNQNRLLEHETLFLGTINSTEVHPREIVKSALRHNAAAV
                     ILAHNHPSGVTEPSRADRLITTNIVKTLDLVKVRVLDHFIIGAGDVLSFAERGWL"
     gene            complement(29620..30438)
                     /locus_tag="J1Y83_001583"
     CDS             complement(29620..30438)
                     /locus_tag="J1Y83_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000761991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF945 domain-containing protein"
                     /protein_id="HBA7629738.1"
                     /translation="MNLASRFGRVNQIRRDRPLTHDELMQYVPSVFGENKHESRSERY
                     TYIPTITLLDNLQREGFHPFFACQTRVRDVGKREHTKHMLRLRREDQINRNQVPEIIL
                     LNSHDGSSSYQMLPGLFRSVCCNGLVCGESFGEVRVPHKGNVVEKVIEGAYEVLSVFD
                     QVEEKRDAMQSLMLPPPAQQALAKAALTYRFGEEHQPVTESQLLAPRRWQDESDDLWT
                     TYQRIQENLIKGGLNGRTAKGGRTHTRAIKGIDGDVRLNRALWVMAENMLQLAS"
     gene            complement(30675..31628)
                     /locus_tag="J1Y83_001584"
     CDS             complement(30675..31628)
                     /locus_tag="J1Y83_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000290407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA7629739.1"
                     /translation="MAVNVRSSIAVKDIRTSRFLFWYIRKNIQGANQFAFDGNNPDTS
                     LSERIINTALEAGYPDPIQRSNFIRSLELSINTMLLPEKYFSWLKENERATFWLWGVM
                     CLDYRCMESINEKLGTNLNINWYQKAGITDSPASHSERYSIIVALLDYINIETNQAPL
                     MRQWLYERLVFWRANEHQRIKLRWLNEDNDEVCIWAYNYINKFQKEHGSNTGSQLDPV
                     QIPEPLNSVETYHAVYAMLDLWSANDDLQQKAVKKLNKAFYQKNFRRKLSEKRERESI
                     SDTHKERLDFLVEFYKSDKISVIERLIDERVQGIETRLSRG"
BASE COUNT         8940 a         7271 c         7428 g         8272 t
ORIGIN      
        1 tatatcatgt gatatgttct gtaacaagta aaatcaacat aaaaaattca gcaaaattta
       61 atatttgttc aactagccgt ctcacatatg acattattag tcaactgcat ttgccacaaa
      121 atggctattt catggaacac agtaatttct tagtggctat tatcatgcta atgcttaggt
      181 ccctagcgat tattcctgcc tggttcgtca gcttacgcca tgagattgtt acattccttt
      241 ttcagactgt gtatttcctg gagatatggg ggaacgcagg agacgctttt tcatctacat
      301 tcgttggtaa atatggcttt ggtcactgcg acttaaacct tcctcaatca cagctattcg
      361 ccgggcgata cggcaaacaa ccaatctgaa acatcatcct gaaaacacca acatctacaa
      421 gcctttccag atcgacttca ggggtgacca cttacaagcc aaaaaccaag aaacatattt
      481 catgatgaga attatgatca tcctgatggc gggtaaacac ttgttttcac cacgctcact
      541 gagttagctt gcaaagcttc ctttgtttag accataaata aattttttat aaaacaaatg
      601 gttaaaagaa agcctttagt ttttgaagac cacctcaagt gttcgtctgg cttcacatgg
      661 catcttcctc ttagaaaaag atcgacatat tttgtgacac gaattgcaaa tctcgttttg
      721 ttgtatggat tgcgtgattt ttgatctggt ataacaggta taaaggtgcg ccgcgatagt
      781 caatgagaca gggcatctcg caatctatgg caaacatcac ttcagttctt tctcatcggg
      841 tgatgaaaac gcacttcagt ctgaaaggaa tatgaaaatg agatcaacag acactctatt
      901 ttatgactct gggtaaaatg gattgagtaa gtgatatagc ttacgaacat tcaaatcaat
      961 taaacatcag aagagatttt atactcaggt atttaatctg gttctctgtt tatttaaata
     1021 atgtggaaag agaattttca caggagacct tatacaaaaa atataaaata cagctactcg
     1081 ttgccaaaga cactataagc ctggcaaaaa atattacaca acataaatgc taattgttta
     1141 tgcgggcttt gtattgcttt ctgtatccta caaatgagtg aaatatatga aaaaggctaa
     1201 aatactttct ggcgtattat tactgtgctt ttcgtcacca ctgatttccc aggctgcgac
     1261 actggacgta cgtggtggat atcgtagtgg aagccacgcc tatgagactc gactcaaagt
     1321 cagtgaggga tggcaaaatg gatggtgggc aagtatggaa agtaatacct ggaataccat
     1381 tcatgataat aaaaaggaaa atgccgcact caatgatgtt caggttgaag ttaattacgc
     1441 gattaaactt gatgatcaat ggacggtgcg cccgggaatg ttaacgcatt ttagcagcaa
     1501 cggcacacgc tacggaccct acgtaaaact gtcctgggac gcgacaaaag atcttaattt
     1561 tggcattcgc tatcgttacg actggaaagc ttaccgacaa caagacttat ccggtgatat
     1621 gtctcgtgat aacgttcatc gttgggatgg atatgtcact taccatatta atagtgattt
     1681 caccttcgca tggcaaacga cgctatacag caaacagaac gattatcgct atgcaaacca
     1741 taagaaatgg gcgacggaaa atgcatttgt tctacaatac catatgacgc ccgatattac
     1801 gccatacata gaatatgact accttgaccg tcagggtgtt tacaacggca gagataattt
     1861 atcggaaaac agttatcgca ttggtgtgtc atttaaactg tagtagacag gagacagtca
     1921 caatgaataa aacaataaca gcgcttgcta tcctaatggc ttcatttgcc gcaaacgcgt
     1981 ctgtattacc ggaaactcct gtgccattta aaagtggtac cggagtaatt gataacgaca
     2041 ctgtctacat tggtttaggt agcgctggta cggcatggta caagctggat acacaggcca
     2101 aagataaaaa atggacagcg ttagctgcat tccctggcgg accgagagat caagcaacct
     2161 ctgcatttat tgatggcaat ctgtatgtgt ttggcggcat tggcaaaaac agcgagggct
     2221 tgactcaggt atttaatgat gtacacaaat acaaccccaa aaccaatagc tgggttaaat
     2281 tgatgtcgca cgcgccgatg ggcatggcgg gccatgtgac ttttgtacac aacggcaagg
     2341 cttatgttac tggcggtgtt aaccagaata tcttcaatgg ctattttgaa gatctcaacg
     2401 aagctggaaa agattcaacc actatagata aaatcaacgc ccactatttt gacaaaaaag
     2461 cagaagatta tttcttcaat aagtttctgt tatcttttga tccctctaca cagcaatgga
     2521 gttacgctgg cgaatctccc tggtacggaa cggctggtgc ggcggttgtg aataaaggtg
     2581 ataaaacctg gcttattaat ggcgaagcca aaccaggatt gcgaacggat gccgtatttg
     2641 aacttgattt caccggtaat aatttaaaat ggaataagct tgctcccgtc gcatcaccag
     2701 atggcgtcgc tggcggtttt gcaggcatga gcaatgactc acttatattt gccggagggg
     2761 ccggattcaa aggttcacga gaaaattacc agaacggtaa gaactatgcg catgaaggcc
     2821 tgaaaaaatc atatagcgct gatattcatc tttggcataa cgggaaatgg gataaatcgg
     2881 gcgaattatc gcaaggtcgg gcctacggag tatcattgcc ctggaataat agtttattaa
     2941 ttattggcgg tgaaactgca ggcggcaaag cggtgacgga ttcagttttg atttctgtga
     3001 aggataataa agtcacagta caaaactaac gcttcagggc cccagtaaag gggccctgct
     3061 atcaatttat catttcaact acgcgaaaat tatatgaacg cgataatatc gcccgattat
     3121 tactatgttc ttaccgttgc tggtcagtct aatgccatgg cgtatggcga agggctgcca
     3181 ttaccggacg gggaagatgc gcctcatccc agaattaaac aattagcgag atttgcgcat
     3241 acgcatcccg gaggcccgtc atgtcacttt aacaacatta ttccactgac tcactgccca
     3301 cacgatgttc aggatatgca gggttatcac catcctctgg caacgaatca tcaaacacag
     3361 tacggcaccg ttggccaggc actgcatatt gcacggaaat tactgccctt tattcctgat
     3421 aacgcagggg ttctcatcgt tccgtgttgc cgtggcggat cggcttttac cgcgggcagc
     3481 gaagggacat attcagaacg gcacggagcc agccatgatg cttgtcgttg gggaatggat
     3541 actccgctgt accaggattt agtcagcaga acgcgagtcg cactggcaaa aaatccgcag
     3601 aacaaattcc tcggcgtatg ctggatgcaa ggcgaatttg acttaatgac cagtgactac
     3661 gcgtcacacc ctcaacactt taatcatatg gttgaagcct ttcgtaggga tctaaaacaa
     3721 taccattctc agcttgataa tattactgac gcaccgtggt tttgcggcga taccacctgg
     3781 tactggaaag aaaatttccc taatgcgtat gaagcgattt atggcaatta tcaaaataat
     3841 gttttagcca atattatttt cgtcgacttc cagcaacaag gtgaaagagg actgacgaac
     3901 gcgcctgatg aagatccgga cgatttaagc acgggatatt acggttcagc gtaccggtca
     3961 ccggagaact ggacgacggc actgcgaagc agtcatttca gcgcggcagc ccgtcgggga
     4021 attatttctg acaggtttgt agaagctatt ttgcagtttt ggcgcgaaag gtgagcgttc
     4081 attaatttga tttattcgca tccatcaacg cttttaactc ctccggcgta aacggtggtt
     4141 tgcgtcggtg caacatcgca tggcaattag ggtacacagg cagcaaaaaa acaaatgaga
     4201 tcaaggtaat atatcttcac ttgcaggctt gaacagcaga aaatttgcga gcatgatcgc
     4261 aattatttca ctgccaggga tatcaatggc ttccagtaaa tcgctacagc aggcaattgc
     4321 caatataaaa atctggcaca aaggtgaaca gcgcgcgccg cataagccat tgttattgct
     4381 atacgtatta gcgggatacc tgaatggaca tccacgcctt ttcgattatg gctcggaaat
     4441 ctacgaacct ctgcacagtt tactggaacg ttttgggcca cagcgttcgc agtaccgacc
     4501 tgatatgccg ttctggcgat tacaaggcga cggattctgg caattacaca atgccgggct
     4561 ttgctcaaca gcagggagca gccgacaacc accagtgaag gaactgactg aataccacgt
     4621 tgcaggcggt ttcgatgaac agcactacgc cctggtaacc ggcaataaaa aactcatcaa
     4681 taccctggcg caacagatac tcgaagcgca ttttaccgag agtattcagg aggaaattgc
     4741 tgacgaattg ggttttgacc tccagcaaat ccgtaaacag cgcgacccgt tatttcgcaa
     4801 aaatgtatta cgcgcttata actaccagtg cgcgatatgt ggctttaata tgcgccacga
     4861 cgacaccacc gtcgcgctgg aagcggcgca tataaaatgg aaacagcacg gcggtccttg
     4921 tgaaattcct aatggcctgg cgctttgcgc aattcaccat aaagcctttg ataaaggatc
     4981 gattggcctg gatgaaaata tgcgggtact ggtatcagat gcggtaaacg gcggagggat
     5041 cgttgagaga ttattctggg attttgacgg gaaaactatt gcgttgccgc aggtgcgtaa
     5101 aaattaccct tatgaggggt ttgtggagtg gcatcggaaa gaggttttta gggggtgagg
     5161 ttgataacag cattcaccct acaaccttac attgtattta aattagagct gagttaacag
     5221 atagttgtat atataaccgg cacttgtcac aacgattaac tcaatatagt tagaatacct
     5281 catattaatt aaaaaataag taatgtcaca acagaggaaa agtcacgatg actcttgtag
     5341 atagtgtcga agcaggtagg ctaacgatta gtgaattgat tgatgccctg gcgaaagata
     5401 aaaattacac agcttccaga tggtatcagc gataccgtgc atttacgact ttgctacagc
     5461 aaacctcaac ttttgctgag cctgcaacag atggtctggt caaacagctt tggtatgagc
     5521 gtgacaacgg cattgcaagt attcgccagg gcgttccatc cttagcagaa tatcagcaaa
     5581 gcctgccact gcttagagaa ctaactgaac gaattcggca acagccggat gaagaaactt
     5641 accaatatgt tggcaatgca cttaaacaag ctaaagaaaa cggacttctc aagcgtatgt
     5701 attggagttt gagaaatcgc gtctttgccg cgttctcgcc agaaaactac accagtactg
     5761 tggatgagaa tgcttttaat aaagcagcag aattcttaaa tcagcacttc catctcggtt
     5821 tggtactgac cggaaattgg ttacagaaaa actatgaagt gaaacaagcc atacacgccc
     5881 gatctcctga tacagatcct tattacgtga atatggccat ctggcatctc tatgaattgc
     5941 tccgtgaacg cgataatgaa caaaagcagg agaaagtagc tagcactaca tccataacca
     6001 gcagtgagcc catcgagaac aagatcatcc tacattcacc aactaacgtg atcttctttg
     6061 gcccccctgg cactggcaag accttcaggt tgcagcaaaa aatgaaagag tacacttctc
     6121 atgctgttcc cgctgatcgt gatgcctggc tggattctcg ccttgaatcg ttgaactgga
     6181 tgcaggttat aacgctggcg ctgctcgatc ttgggaaacg agcgaaagtt cgccaaatta
     6241 ttgaacatat gtggtttcaa cgtaaggcat tattaaacgg tcgtaatggc aatctatcga
     6301 atactgcctg ggcagctttg caatcctata cagttcccga gtcgttaacc gttgattata
     6361 agaatcggcg tgagcctgcc gtatttaaca aaacagataa cagcgaatgg ttgctagttg
     6421 attcacagct cgagcaagtg gaggatttgg tagagctcta cgccgaactt aaacgtggcc
     6481 ctaaatctgc cgaagccatc cagcgctttg cggtggttac gttccaccaa tcttacggct
     6541 atgaagaatt tattgaaggt atacgcgctc gctctgacga gagtggcaat atctcttatc
     6601 ccattgagcc gggtatcttt atgcgccttt gccaacgtgc gaatgccgat ccaggacatc
     6661 gctacgccat ttttattgat gagatcaatc gcggcaacat atccaagatc tttggtgaac
     6721 tgatctcact cattgaagtc gacaagcgtg caggcatgcc caatgcaatg agcctgcaac
     6781 tggcttatag cggtgatcac ttcagcgtac ccggcaatgt cgatatcatc ggagccatga
     6841 atacagcgga ccgttcttta gctctgatgg acacggcttt gcgccgtcgc tttgactttg
     6901 tcgaaatgat gcctgatctc tctttactga gtggagctaa ggtgaaaggc atagagctcg
     6961 agtcgttgtt agagaaactc aatagccgca tcgaggctct ttacgatcgt gaacatacgc
     7021 tggggcatgc gttctttatg ccggtaaaaa atgcactcga tgccggtgat gaagaagctg
     7081 cgtttaaaca attgaagatc gcattccaga aaaagatcat tccgctttta caggaatact
     7141 ttttcgatga ctggaacaag atccggttgg tgctggcaga caatcaaaag caagacgaca
     7201 acctgcaatt cgtgattgag aaaaccgacg atctcgatac gctttttggt aacaaccatg
     7261 gtttacgacg ccatgatcag caatcaacag cttatgagct caaagatttc gatcaagaga
     7321 tctggaatat tccacaggct tatcgttcaa tttatcagcc ccaacagact ccccttgatg
     7381 agcaggcagt aaatcatggg tgaagttatc tccgtttttg aatatgacct actggggagt
     7441 ggaaaagcgg cttcagttgg cgcaaaaccg gtgcctccac aggtctttaa ttatttagaa
     7501 gcgcttagcc tggcaagtaa tcaagggagc cagttcctca aactcacctc tcgctcaggc
     7561 ttcaaattgc ttcaggttca aaactacgcg gggatgcttt caacacctca cggtttccag
     7621 cttgagatac tgcccaaggt tggtaaaaac ctcacagcgg ctaatgcacg tcaaacattg
     7681 ctgacgatgc taagccatct gcctgggttt cgacatattc aaacccagca agccactctt
     7741 caggctcagc gtatgccttt gcttgagatt tttattaacc agtttttgca tagcgtcagc
     7801 caattgctta aacagggttt acgctccgac tatgtgagca agcaaggtaa tctggctttt
     7861 atgaagggca agctgatgct ttctgcacag ctgcgacata acgcggtgaa tcgccataag
     7921 ttttgtgtcg attatgatga ctacatgcct gattgtgcag ccaatcggct cttacactcc
     7981 gcgctagata agcttcttag tctaaagctg tcatcagaga atcaacgctg gctttacgaa
     8041 ctgcgctttg cgtttgacgg tattccactt agccgggata ttgaacgtga tataaacaac
     8101 ttacgcctcg agcgtggtat ggctcattac aacgagccaa tggcatgggc gcaactgatc
     8161 ctgaggggaa tgagcccgag tgctttgcag gggaacacca aagcgatatc acttttgttc
     8221 cctatggaag cggtatttga atcctttgtg gcacagaccc taccctacga attaccttct
     8281 cacctcaaag ttttacctca agcagcaact tattctttgg taaagcatgg agttaaagat
     8341 tgctttaagc ttcgccccga cttgctgatt caatcaaata aaccagttca aactgaaatg
     8401 gtaatggata caaaatggaa gttggtgaat agcagccagc agacaaaatc actctatgga
     8461 ctggcacaag ctgacttcta tcaaatgttt gcctacggac aaaaatatct tggtgggaat
     8521 ggcgaaatgt atctgattta ccctgcgcat gatgacttta gccaaccgat accgcaggat
     8581 tttgctttct cggagacttt aaaattatgg gttgtgccat accggataat ggcaaaacgc
     8641 ggtgagagga tgatgtggcc gaatgacgtg tcctgtacaa tgagccccaa actggattgg
     8701 atggctgctt cagtatccta tagtggaggc atgagatgac ccatgaatac cagcgacaaa
     8761 gcctggaaca actaattact cagcaaacag caactcccag aagcgccgcc gttgtattta
     8821 atgagaacca aaccctctgg caaacacttg gctttagcca gtctcaagtt tcgctatggc
     8881 ttgcctcatt gcctcaaagt aatcatctaa aagggcccac aacggcaacg taccaggtta
     8941 tacctgatat cgcggcacat ttggtatcat tgttgcatca agctggtggg cgcatgcccc
     9001 ttgcacaagt actgaaaaag ctccctgctg gtattacgac cagtgaacag cagatacgca
     9061 aactggcaca gaagcacgcg cagatagaga ttaaaggccc cttgctggta ctgataaatt
     9121 agccagcatt tgtagtaacc attggctgta ttaaaggatt ttagaaaaca tcatggcaaa
     9181 agaagcaaaa aaacaagacc cgcaacagca gctagaagca ctgttcaata aactggttaa
     9241 acagcactcc gagtttactc agccattggt cgaattaccc gagcatgaat tcgccaaggc
     9301 gggtctcatt caaggcctgg ttgaaggcaa cttaacgcca ccagtcatgt tgattgcgct
     9361 gacagaaggc cattgggaat acgccgctga cactgcaaag tttgatgatt tagcctatca
     9421 gctgttaagt gatatcgaag gtgactggcc agtatatgcc gttgtggacg atggcttgaa
     9481 ccaacgtatt ttggccttgt tcggtgccga tggttctgac ggtacccacg gtgcagatac
     9541 cctgccaggt ctggaagaat tacgtagctt tgatcgcatg gaacgtgatc caacttttcg
     9601 ctggtcgatg cgtgtttata cacgcttaat gcagcgattt gatgcctttc atgaaaatgt
     9661 ttatcgcgtt accaaagaca aggtaaacga caagaacgac attattgagg aagtggctaa
     9721 acttctgttt ttagaaactt tccgcttgca tcacgatgaa gaccttacct ttaaagatga
     9781 tgagggaaat accctgcgtt ttcgtgatgt ctttgattgg caatatgtgg agagccacgg
     9841 cgataaagcc gtggcccaaa tcaaagccgc ctttgaggaa tttaaaaacc acgaaaacta
     9901 cgttgtgatc tcagatgatg gctcacgtaa ccccatcttt agtaaagaaa cacacctgcg
     9961 gctgtctgtt gcgaaaaact atcaggattt gctggaagca atacaaaacc tggggccggt
    10021 aaaaaccaac gatggcaaaa tcgctaaaga acacggcacg ttggctgatg tgtcaggaga
    10081 tttactcggc agagtgtttg atgtattcct acgcgctaac tttgaatcca aaggtggcct
    10141 tggcgtttat ttaacaccaa acccagtaaa acaggccatg cttgaaattg ctatgcacga
    10201 catcgacgat gacgatgaaa tgcgatcgcg cttagccaat ggcgattttc gtttctgcga
    10261 ccccacctgt ggctcctttg gttttggctc ggtggcgtta agccagattg ataaatggat
    10321 cgattttaag ctggtgctgg ccgacgataa aaaagaatca ttgaaacaaa aattacgcga
    10381 ctgtgcattt accggtgccg atgctgcacc acgcatggta atgctggccc gcgtgaatat
    10441 ggcattgcaa ggtgccccca aagcgcagat tttctatacc gacaactcac tcactactaa
    10501 cgccctgaag ccgaatagtt ttgaccttat ttgcaccaac ccgccgttcg gtacgcctaa
    10561 atttgataaa ggcaaaaatg gtcaaaactc aaaagccaat tacgaagcca atatggagca
    10621 agtgctcggt ggttttcgcc cgaccaaaaa agtcgttgat agctacaacg cctggtatga
    10681 ccacgtgaaa atgaagtggc aggatatcgg tgatttagag ctggatgaaa acggcgagcc
    10741 taaatgggcg ggctatcgta ccgatttgcg cagaacgggc ggtactgata aaaagccgtt
    10801 ttacagcctg cagcctacaa ccgcagggct cgcgctgggt agcaagcccg atagcaaggg
    10861 caactggcaa ccggtaggtg ccactattga ccctgcagta ctttttatcg accgctgcct
    10921 gcaactgctt aaacccggag gtcgcctgtt aatagtgctg cctgatggta ttttatgtaa
    10981 ctccggcgac cgttatgtcc gtgagtacat tatggggaaa aaagacgaaa aaaccggcga
    11041 gtttgttggc ggtaaagcta ttgtaaaagc agtaatttca ttgccgagcg actgctttaa
    11101 actctccggc acgggggcca aaacctcgat tttgtattta caaaaacgcc atgctaaccc
    11161 caaccagcct gagcaatttt tacccgagcc acaaaccgac gtatttatgg cagtggctga
    11221 aaccttgggc tacgtggtga aaaataatat tgaagactat aacgctggtg tcgcaaacga
    11281 tctggataag attgtatcgg cttataagcg gggtgtgtaa tgtctgtaga taccttacgc
    11341 gaaacatttc atataaaccg cattagcact tctgatttgc aggatttctt gactgcgcaa
    11401 acctacagac cagaaattac gcaagcaaaa aatcatattc ttagcttgaa aaattgctca
    11461 ctgcaggcgg tatgcacaaa accaattcag caaggaaagt cccctaagta tgcggagaag
    11521 ggcttaaagt gcattaaacc taaaaatact aatgacatgt tagtttcgat tgatgacatt
    11581 gattggattg actcgtcaac aaaagatcaa attcaaaaac agaagctcgg atatggtgac
    11641 attgtcatta cacgttcagg ttcaggaact attggccggg catcgattta ttgctatagc
    11701 gaagaagcgt atacgaatga ccatcttttt gttgttcgcc ctgacaaggc tgatagtcat
    11761 tatatttgct catttttaaa tagcttccat ggccaacgct tattggaagc aggggtatcc
    11821 ggttctaccg ggcaattaaa tctttctaat gaacacatta agtctattcc gctgtttaga
    11881 cctgatgata aagtacaaaa atacatcggg gataaagttc gccaagccga acagttgcgc
    11941 gcttgggcga agcgccttcg cacatctgtt gacgatcatc ttaacagtct aaatctgcct
    12001 atcaacgagc cccccgcact gttaaaccga gtcagcgcgc aaacaatgga ggaccgactc
    12061 gatccacgcc cttatcggac tcattaccta tgtttagtga gtgaaattga aaagctacct
    12121 catgacagta tcggcacttt agttgcattg gcatcaggct acccagtatc cagtaacgac
    12181 tttttggaaa actcaggcat acctttagtt cgcatccgca atatcggttt tgatgatttt
    12241 attggtttag atactggtgt tagccaagac gtatatcagg atgcgactaa gtatcaagct
    12301 aaagacaaaa tgatcgttgt cggtatggac ggtatttttc gctcgcagtt tttcatatct
    12361 gacgagctac ctatgctagt taatcaacgg gttgcaatgc tctcacctca aaatattcgt
    12421 ggtgaacttt taacacattg gctaaatcgc cccgaaggac aaatgcaact aaatcaatgg
    12481 gcagttaaaa caactgtaga acatacatct ctatctgata ttggccgtgt attaattcct
    12541 cgcctggata aaagcttaga aaataagctt gctgattatt tgctcaatgc gaggctggct
    12601 tatcgttatg ccaaattttt aacacaagta gccaaaaccc tggtcgaaac cctaatcgaa
    12661 ggccaactga ccgagcggca attgatcgaa gcccaacaag ccctggaaga tggcgacaac
    12721 agcttagatc aagcgatttt aagtaaacta agtgctgagg gttatgccat agaaggcgcc
    12781 acaccgctat ttagcgatgt ggatgagtta tacagtttgc tggaagaggc ggcgcaagcg
    12841 gaggctgaag agtaaagatg gagataaggg gcatggacag cagtacaacc aggcaacata
    12901 acgaccccgt aaatagcgaa gcggcactta acctctgcct gcagctttgg caacaaggtg
    12961 gtttgaacgc caacaaagca gcgttgttac tggcggctgt gcctgccctt aggtcactgc
    13021 tacaaccaat aatactgccg caaaaaaacg acgctgaaac ggatatcgtt agcgccttta
    13081 gcctcactgc accattgctt gatgccttta acgatttaag ccagtcaggt gaatggcaat
    13141 tagcgttgtt gggcttaaac ccagacgttc gccaacattg gataaattta gccgccgctc
    13201 gctgccagga agcaggcgcg atgaatgaca taatggtatt ggttaaactc attcagcaac
    13261 tgggtaatgc cagtgaatgg gtcttagccc aactggaaag cacggccacc acgccgcaaa
    13321 tcattgccgg ccctctggca aaaaccgaac gcgatttgct cggtcatagc ctaaacgaca
    13381 atgcagccat tcccgctcta tgccgtattt tgcacaccag ccataccttg tttactgtaa
    13441 gtgaacaaaa tgaaccacca gcacctatac aagcggtgga tgtcaccgca aaacaactga
    13501 caaataactg gtgtagtggg cggttgttag cactccctaa taccttgctt gatgaacatg
    13561 accttaaacc caatgccgat tggttattgg tgtcccgctc gggtcatgac aacgtgccgc
    13621 ttaccgagct attcgcccaa cagccctggt tatttttact cagtctcatt atctttgtgc
    13681 aagatgcctg ggctgccgag caacgtggcg gtttgttgct caccttacct gccgggcaaa
    13741 atgcctttgc ccccggtcaa ataaatgtgg cggtacaggg cattgaaggt gatgaggtga
    13801 gtctgggtag tttggccgag tttcttgtgc ttctgctcgg tgagctcaat atccccctct
    13861 atccggcact cgatgcgaac acggaaagca tcaaccgcct aaaccgcgtc ctgagctcat
    13921 tcattgccga attgttggca aaaaaaatct ggcaatttac cgaggcgggt cgtggtgagt
    13981 ccggccaata tcgaattcat acaagcttca gcgatgcctg ttatagcctg ccgttagcac
    14041 cactatttgg ctacaaaagc caaacgttac aacgggccat taagcaactg gcgcaaaact
    14101 gctatgccaa taaaaaacgc gccgccaata gaataaacct tcaaggatcg tcgttatgag
    14161 ttttgaatta aacgaacaga tggttgatag cctggcaaaa caacatcgct gggcggcgac
    14221 gggtgtatca gattatccac ctcgcgaccc gttagtgggc cagagccgct tttttaaacg
    14281 ctttcaaacc tttttgcata cggtggatca cgacgatgat cgctttgccc atgtgtttgc
    14341 cgtcgaagcc gaatggggtc ggggtaaatc acgtttaggc catgaattgg tcgcacaaat
    14401 taacgactgc tccaggggct ggtttgtccg cgacgaacaa ggccaactgc acgataagca
    14461 gctgtttaat cgcgacacgc aagacaaata cctggcgctc tacattcgct actcgcaagt
    14521 ggcatcagat tatcaaaact cggataactg gtttgctttt ggtttatatc aggcgctatt
    14581 accactggcc acccaaacct ttgatggatc gattcaaagt gaaattgcca aacaagccta
    14641 tgaacgctta atgccatttg gctttaaatc tgaactgtta gcagatgccc tacagctgaa
    14701 tgccaaccac agcgaagaag acctctatac tgaccaaacg ctggtagtca gtttggtgca
    14761 agccgcctat aaagtattaa agagttttgg cgtgcagtat atgttggtgg tgctggatga
    14821 gctggaaacc gttgcagaag ccgccacttt tggcctggaa gatgaacaag ataaacgcct
    14881 cgatggccag gccattcgcc tgattggtaa agcaattaaa gaggaagacc ctcgccgtaa
    14941 acttccatgg ttgcgctatg ttgcactgtg ctcaccacta cttggccagc aattgcgcga
    15001 aattcaatct gtcgcacgcc gctttgagtt agtagaactt gagcataatg cctttgccga
    15061 tgtatcagac tatgtggcaa gcctcttaaa agacggcaag ctgggtttta attaccctgc
    15121 tggtttagtg gaagctgcat acgccatgag tggtgccaac ttcggttggt ttaacgtggt
    15181 catggctaat gtggatgcag tgctgagcca ataccaacaa gcaggccgca gtatcaagga
    15241 tgtgggcgat ttatttgaag ctgtgctggc aggctctggc cgcgtggcca ggcatgtgct
    15301 ggataaaggt gcgatagaag gcattcaaac ccgcgaccag gcactattga ccgaatgccg
    15361 tactttgcta tacggccaat tacccgtgcg cttaactgaa gtgagccatg ccacagaatt
    15421 actggcgctg aaaaacgaag atctggaacc catagccgct cattaccgca aactaagcat
    15481 tgaccgccta cagtgccgac aagccctgga gcaagccaaa ttccgccgcg ataaagatga
    15541 atggtactac cctgcagtag atcaagcgct gaacttagat actttgctgg ataacctgcg
    15601 tactttttcg attaaagaac aagccgcaag cggtgataaa ggcgcagtat tactgcctct
    15661 gtcaaaaggt gagtttaaat acttactcag cttgctgtat gaccacccag cgattgaatt
    15721 cgctgccgat gccttatggc acaagctagt gggtgaaact accgaactgg acgccagcga
    15781 agcaacgcat attggcccca gtgtggcgat gttactgcgc ctggatttgc gctatcgcag
    15841 tgcccagcag aactcactga tttttaaaga tcccgccatg ggcgacgctc atgaacaggc
    15901 aatgagtgaa cttagcaaac aaagtagcca gaagccgctc atcaagctat tagctcgtgt
    15961 aacgggtttt gtgagattac tggataacaa ctggacctat gatgaaaact tactgagtaa
    16021 taaagcgggc agcgacgatg cacctttggc cattttaacc gagccccgtg gccgccaggg
    16081 tggtttactt accctggatg gatttaagct tcacccagaa ggtaaagcct tgtttgcctg
    16141 ggtcagcaac ctaattgagc tgaacaatct tcacaccttt gcggcaaacc acagccacca
    16201 aaatggtcgc acgccagtat tagcgttaac tgcttctgca catctcatgg agcaatacag
    16261 ccgactggat gaaagaaatg agttgcgtga tgcgatactg ctgcattatg tcaacccaag
    16321 cgaagcagac cagttagagc gtattggctt aagtttggca gcatgtcagt tgcacggcgt
    16381 gaatttaact gccgacagct ttaccgcgaa attcaaaaac aagctgcatg ccttgacaac
    16441 ctttgccacg gacgccattc acaagtggcg ccagcgttta cagcagcgcg gtttaattgc
    16501 ctggccgtta aaggtagatg gtaaattatc atcgaatgac cgagacctgt tgtttaaagg
    16561 ctggtatcag ctggccattg ctcaccctga gttaaatggc attctcgatt tacagcagca
    16621 acacggcgtg ccagtgaatg agctgagcag cttgttggat aaacttaagg tgccgggcag
    16681 ctatattgcc aaaggctaca cggccgatga acatgccggg ctatttactg aacttaataa
    16741 tgtgcagcga agccaggcgc agatcccatt atttttagcc cgtattgctc accctaataa
    16801 aaagcacaag tggcaattcg aagggtttaa gcaacagttt tatttcgcct atgtggctga
    16861 aacgagtgtt accgccaaag gcgtgtttaa tgactggatg tggtggtgtg gagagctgaa
    16921 cttactcact ttaaccaatc caacagaaaa acaggcaatt tgggagcact acccgcgctc
    16981 ccgcttagaa aatgccatta gagaagcgca aaactggttc cgcggtaacg atatgggcag
    17041 ctatgcagcc aatgtggaag tcatgagccg cgtgtatggt tatgcacgca ttaatgaaat
    17101 gttcgcgcct ctgggtaaaa ataagctggg tttcgtcacc gtggaagcta aagagcagct
    17161 ggaaaaagca caatcgctat ttaacgtgct caaacagcaa gaagaacaac tggctgatat
    17221 ggtcgaggcc aacgacacga aagtattggc agggcttatc cacaaacgcg ctgaagtgct
    17281 ggagctggta gcaaaggtta aaccgcttaa tagttccagg cctatgctaa aagatgccca
    17341 tattctcagc ttagaagaca aaacgacctc gctttatcaa cggattgaac aggcctgttt
    17401 atttgccgag tttgttgagt acagcgctga gcgtatcaac aaccgactcg ttgatttaat
    17461 catagatgtt gaaacagagt gtgccccgct gacgaatttt ccgaaacgtc tgtacaccaa
    17521 cacgctgcgc accataggtc atattttaga tggagcgtta aaagacgata ccagcagtgc
    17581 cactggccga aaagagcagc aagcagatag cgatacctta ctgcattacc tgcgtaaact
    17641 cgatttaggt agagcgcatg acaagctttc agccttagcg caagaagtgg gtttaaacct
    17701 gcacaatgat caacagttgc cgatagcgga gattcagggg catattttgt ccagctaccg
    17761 caactgcaaa gaacgattta gtaagctggt taataacctc acagagcaaa aactacgtgc
    17821 acaacaactg caagaatggc ttagtagcgc cacggctgag tatcagttca ccgatgacat
    17881 tgcagagcta ccaaaactgg taatgaaatt acagctcatt gaagatgcta ttgccgatct
    17941 ccccaacgat gccgagagca agcgtcaaag tatgcaaaac agtttgcgca atgggcagtt
    18001 ttcatcgtta cgggatttac cagaagagct tttaaaaccg gcacgcggtc aacttacacc
    18061 gattcaaggc cagctactta aaattgaaga gcgtctaaac caagtaagac gcaactctat
    18121 cgcgcaggta aacagctggc tgccgttatt aaaaccgcta ttggcttctc aaaagcaagc
    18181 ggaaccagca gcgttgactc tggaggatgt gagcaatttg ggtgtcagag aacttcaaca
    18241 ggtgtgtgaa agcacccagg ccaaatggca gagtcaaggt gagcaaatat taaatgatac
    18301 aggcttaaca ctggcggatt ggcaaccgat ttatcaggct ttgagccaaa atcaggaacc
    18361 cgtgttaaca cctgagcaac agaaaggact tgtagacaaa ggcattgtaa aaatgcgcct
    18421 tacctttgcc actggtttat agaggtgacg cgatgagtct ttatttatct gcggcactgg
    18481 ccagtaatcg taaaggccgt tttttgcaaa ctgttgcggg ggctactccc ttaacgaagg
    18541 attggattag ctcgccacca gccagcggtt tgttaataat gcaagcagaa gagttgactg
    18601 acgctaatac tatgcagcac ctgtatcact gggctatgca ggcaggttgt gccgctttgg
    18661 taataaacct gaaagcagaa caatttacct tattagcgca attgccctat ccactggatt
    18721 ggcagttagt accagcgagc ttgaggaggc aggaaccggg gctaaccgct ttgctagcca
    18781 gtgaaacaga ccaggctatt gctggcttta ctggcagcgc agatcgctac cagcaccagg
    18841 ccggtgatgt agtgcatacc cgctacatac gaaaacacag caacagtggg ctattagcct
    18901 ttaccacgtt acctttgtgg tcgctgactt tactcgatca tagtgagctc ctggtgagct
    18961 ggttgaattg gtttgtggac catgcaggca ttgctgaacg aataatcgaa cctaaagcgc
    19021 catcaactga ctacacgcca gataagcacg atctggttgt attgctatta ctttacgctg
    19081 gcggtggcat gaacttacaa gcgttgtctg agcataatgc ggttaagctg atgtttgatg
    19141 ttaatagcct tgatattgtc aagcggggtg agatgttgcg gcagcacgat tttatcgacg
    19201 acgcgggcat aacagctacc ggtaaaacat gcctacaagc ctcgcaatat tgggcttatg
    19261 ccccattact tggcgagcag ctacacacag gaacgctata agcatgaatg agaacaaaac
    19321 gatgaattta gcgcaatcgc tcgccatcga tagccagctt cgcttaccgg gaagtgcagg
    19381 acgcttgctt aaacaggtcc gtgatattga gcaagtggcg ccgctattcc gtgatgctgg
    19441 cttagtgaaa gcggtcccca agcccacaat tgttaattta cgcgtagccg gtattgcgac
    19501 taaatcgtgc ttagacaagg tattgggtgg ttttatttat gccagtgcag cagtgcgcac
    19561 agatttgtta atagatgaca ataaagtcag tacggtgggt agcgactcgg tatcagaact
    19621 ggatgatatt gattttgaag ctcagcgtaa acggcttgat ttaattgaaa ttcgtcaggc
    19681 ttatcagtta gttgagaaaa aacttttaag ttatgagcca ggcgatttaa ttttgctgga
    19741 tacgccgttg tttttagcac gggatatggc tccgctggag agaaatgtcc gtcataaaca
    19801 agagtacgaa aaaaccagac aagtgataga aactttttgg caaaaccatc gctccagaat
    19861 ttttccgtgg aatcaaaatg gtgtagtact tgcgagcatt ttggctgagc gctttagtgc
    19921 cattgtaagt atcgccaaac aagatttacg taccgaacaa ggccgcaaac aaattttagc
    19981 atctgatggg ttttctaaag agttgatgca aaatctggaa ggactggatg aaagtttggt
    20041 tggtgtgggg gatttgcgat ttatcaatgg cattttaggc aactattcgc gcaccatcgc
    20101 atttcgtttg tctgaacaag aaaagcgcat tgaacccaac attgaagcta aacaaggtct
    20161 aattggtttt cacttccgta gtgcccgagg cggacagata aaaatggtgc aattagcggg
    20221 tgacgaacca gaatggaaaa gtgctgatct cgatttagtt gcctctcgcc tgatggtgct
    20281 ggatatgcaa aacaaaggta atgcaatgcc gttgccacaa ttacttggct atcaacagct
    20341 tggtatttta cctaaatttg ctacgtttta tcgccagggt ttacaccagg cactaaagaa
    20401 taatgatatt gaggcagggt ggttggcagg cctcgatgag ggattgaata atggcttata
    20461 aagttttagg taaattagtc ggtaacactg gtgatgccag tcagttaact atggttgtac
    20521 aagactcgtt ttcagtacgc cgtggtgagt ttgtacgcat tatgcaccag gaacgcaaag
    20581 atgaggtgca agttgccgta ctggggcgag tgaccaagat aagccgtact aatatgctct
    20641 ataacgccgg ctttggcgat ggtgtaaccg agcttgagct tttgcccggt gcgaatgtaa
    20701 ctggtgagaa tatgtttgct caggtcgagc tggttggtta ccgcgatcct gttactcgtc
    20761 aaatcaaaat tccacgtcgc ccgcttaacc cgggtaccgc tgttgaaacc gtcgattttc
    20821 agtttttgag tgatttttac gaatttaatg aacactccag tttgcactta ggtaacttag
    20881 taggctacga caaaggtgag aatacagttc ctgtttttat tgacgtgaac aagttggtca
    20941 ctgagcattt ggctgttctg gcaatgacgg gttcaggtaa gtcttataca gttggccgca
    21001 ttattgagcg tttagtcgcg gttaataacg gtacggtagt agtatttgac ccacatggtg
    21061 aatacggtaa agccctcgct ggcggtaatc tgcaattctc aagcttctta gaagccactg
    21121 acgataagcg tgatcaagaa gcactgccac tgataaaaca gacctttgaa aagctgcaga
    21181 ccgccggtgc aggtattcag gtttactcgc cgcagcacga atcttttaaa cataaatacg
    21241 ccagtaagaa tacgccatta gcgcttcagt ttgaccattt tgaaatggac gatattgctg
    21301 aaatattacc tggcttaacg gaaccgcagc aacgagtact cgatgttgca attcgttatt
    21361 ggcgctctgc agataagact gagccacgag atattaaccg cctacgcttc ttattaggtg
    21421 atggtattga agagttaaaa gagtgggacg acctttctga agctgaggcg aaggcgttat
    21481 caggccgtag tgccgccgtc gcctcgatga agctttcacg tgtgcttaat gaagctaaaa
    21541 gtttttacag cgcaacaatg gctgagccaa ctgatattta caaaatggtg ggtcgcccga
    21601 gcaatcaaca ggggcgttta gtcgttgttg acttacaagg tttgagtgat accgctaagc
    21661 aagtcatttg tgcactgtta tctagtgaaa tattaaaagc agcctcgagc aaaacggacc
    21721 cgctgcgccc atgctttatc atttacgaag agggtcataa ttttgccccg gccggcggca
    21781 acgcagtcag tcaccgcatt attaaaaaga tcgctggtga aggtcgtaaa tttggtgttg
    21841 gatttggcat tgtgagccag cgtccatcga aattagattc agatgtgacc tctcagtgca
    21901 atacgctaat tacgatgcgc ttaaagaacc ctgacgatca acgatttata gctaaagcat
    21961 cagatatggt gagcaaagct gatctggagg aactcccgag tttatcaacc ggtgaagctt
    22021 tggtgtgtgg tcgttcaatc cctgcaccac tgcttgttaa agttggtagt aaggcattaa
    22081 ttcacggtgg tgaatcacca gaggtactgc gtgtttgggg ctctttcagt ggctaaggtg
    22141 cctatgcctg aagttgcctg ggtaaatgag acatttccac ttttagcaag cggtaagcta
    22201 ttggaccagg atgtattggt ccacagcctg ggctgtagcg tttggaatac attcgggcat
    22261 gaacagtcat ttatggcggt agtggaatgc ccggctccaa ggacatttgg tgctgatatt
    22321 cgctctgact cgggctggtt tcataaatct tctgcatcgc ctgtgtgttt aatcgaattt
    22381 gaacgctttg atggttcagc gaaggggcag caaaagctgg aagaaaaatt aaaaaactta
    22441 ctggaagcag cacagcgctg gaatcattgc cccaaaaccc tggtcctcag tgcctggagc
    22501 caaggcttag taggtgtacc tgatactcaa aaactgaaag acatatgccg tatgggtttt
    22561 acttcgtcca cgggtaccca agtaatcgcc gctcctgatg ttgaggtggt atttagtcgc
    22621 tttttattta tcaaaaacct gaacatgatt gttttggaca gaattcacta tgaggtgttg
    22681 atgtgaatat aaaatcagca cattttttgc cagggacgaa taaacgcctt ggtgacaaat
    22741 acctgttact tgaatgcctt ggtgatggca gccatggctg ggtatggcgg gcggaacgcc
    22801 tgaaagatgg aaaaattgtc gcagtaaaaa tccctaagga tattactcgg gaagaccgtc
    22861 agcttgccga aggtaaagag cttttaaacg tcgagccgca cgaaaatatt gtgcaaattt
    22921 ttgatatggg ccgtatagat aatgaatggt tctatattga aatggagtat tttcccagcc
    22981 aaaccctggc gcaaaagctc gatgacaggc agcgtaattt tggccagacc tatgaacggt
    23041 tgttcagaat atatcgtcaa gtgctctgtg ctgtgcatta tttggccgaa ttaccagtgc
    23101 caatatccca tggagatatt aagccacata acattttggt tggagagcgg gacctggtga
    23161 agttgacgga ctttggcagc tcagctttac cagaagaaat ttacgttcgt acccgtgaaa
    23221 atggcgggac tgtgctgtat tcggccccag agttctccaa cgtcgatagc cgtagaggca
    23281 gcctggaaga gctactattg ggcgacatct atagcttagg tgttttgcta taccagctgc
    23341 tgacaggaaa gctgccccat gatacgccag cacaagttca gcgccacgca cctttcaagc
    23401 ttccgacaga gattaatagc tctattcata cggacctgga acaagtggtt cttacttgtt
    23461 tacaaaaacg agcggaggat cgcttttcca ctatttccga cttaattcat gcatttgatt
    23521 ctgctgccac taagcaatta aaagttggtg ctattgctcc cgtttttcaa actaaacctg
    23581 ctcaggactg gtcaagtgcg gtacttgaag caatgtccga tcaatcttac caaaaagctg
    23641 ctcaattagc gtcgcaggag tatagccgca gtaaagatct acaagcttta catcagcaac
    23701 ttattgcctt gtatcgcgcc aatcgcttat tcgactttga aaaagttgta gaagataata
    23761 aagcgatctt acttgagggt aaattgactc aaccagcagc tttgttcgag ttgatagtta
    23821 aggcaaacct acagttacgc aatatcagtc aagcaaattt ctggctgttg caacgaaagc
    23881 aagctgaagc agaaaacgcg gccaccatgt atttagaatc atctatcctt ggccttgaag
    23941 cgaaatattc tgaagcaaga gtactattgg agaaagtaaa ccaaacaact ccaatgaagt
    24001 tccatgtgtt aagccaactc attttagttt gcgagcaaat gcgtgattac tctggtgcag
    24061 cggcttattt gaaagcggca ttgcgagtag caccattgga taacagcatg aaagagaaaa
    24121 aagaattgta tgcaaagctt ggagttattt gattgaaagg atgaccattg cgcgaacaag
    24181 gcatagtgga tatttaacac ttccgttcac ataccggaca aaaaaataaa gttttacata
    24241 acgatttcct tcttgattga gttaagacaa aagcctctcc ccattagggc ggaggcttct
    24301 cagttataag cctttaatta aaaaagttaa tattcatata cttagctaga attatcgaaa
    24361 agaactatta atctttgcat agtcaaatgg agtgatagcc gccattctat tcgcatccag
    24421 ataatcagcc caccactgca gcatcaacat tcgctcccca agatgttccg ccttatggat
    24481 ataagccgcc cgtactgagc tacgttcctg atgactcatc tgccgctcta ctgcatccct
    24541 cgaccacaat cccgattcaa tcaaagaact acacgccatc gtcctgaaac catgtccaca
    24601 cacttccgtt ttcgtgtcat agcccatcac tcgcaaggcc ttgttaagcg tattctcact
    24661 cattggctta cgcggatcgt gatcaccaac gaagatcagc tcacggtttc cactcatgct
    24721 tttgattttt tccagaatgg tgagagcttg tcgagataag ggtacaagat gtggagtccg
    24781 catcttcgaa ccacgctgag aatgcttaac tccctccagt ggctcacgtt ctcccggaat
    24841 cgtccacatg gcagtatcaa aatcaacttc tgaccagcga gcaaaacgca actcgcttga
    24901 acggatgaaa actaacaatg tgagttcaac cgctagtcgg gttaacggcc tgccagaata
    24961 gtgatagatg cgttgaagca attcaggaat gcggttaagt tccagagcag cacgatgctg
    25021 ttttttcgcc gtagcaaccg caccagcaat ctcttgagcg gggttgtagt cgattaatcc
    25081 actctgtaca gcaaagcgta taatcgcggt agtatgctgc tgcaaacgag cagcgacttc
    25141 gagacgccca gacgactcca ccgctttgat ggggacaagc agatcccgag tcttcagatc
    25201 agcaatgttc cgcttaccga tagcagcaaa gagattatct tcgaggcttt tcaatacacg
    25261 agcgctatgc gatgcagacc acttctgatt gctggcgtgc cactctctgg ctaccacttc
    25321 aaacgttatc acctcttgct catgctctac cttaacagct ttcttgttct cactaggatc
    25381 gacgccgtta gctaacaact tacgggcttc atcccggcga cctctggcat cgaccagcga
    25441 gacttcaggg tatttcccca gcgccagcat cttctcctta ccgccaaaac gataacgaag
    25501 tcgccagtat ttggagccat tagggtgaac caacaaaacc atgccttcac cgtcagtaag
    25561 tttataggct tttgcttccg gcttagccga acggatcttc acatcactca gagccatgat
    25621 gagtatcctt tcaagggccc tgtgtgggta caaacataat cgaaccggga aatacccgca
    25681 gttgtacccg catcagtaag ttgatgtaga ttgaatcagg ttgacttagg ttgagtgaaa
    25741 aagcgaggaa agccttgcgg atactggatt ttaggcacaa aaaaagacgt ccgttgacgt
    25801 ctattgatgt tccgatggtg cgaaggccgc accgcgattt tcgacgtaat caactgaaat
    25861 aattgaaatt taaaaataag agaaccgacg catatactca catgtatact caacgcaatt
    25921 ctctgagatc ttagctagct caaaaattac tcttcatcat cttccacagg ttctaacgcg
    25981 tcaagcgcga ctcgcaccat agcgatagaa ccaatacgaa tgaaagcatc ttctccatct
    26041 tcgtcggtta tcataaaacg atccgtggtt tcaggctcgc aatccagcgc gtcaaaaaaa
    26101 gcattcaact ggccgatatc atccgtctct tcattttgtg gcgcatcagg ctcaacactc
    26161 agtggaatca ctggtccgcc gtttaccagc gtaatatgaa cattaaaata atcgtcttcg
    26221 taggcatcat cctcttcgtc aacgattggc cgcagaccat cagaaagaaa ctgatgaaat
    26281 accacttcac gcatatttaa cgcataacga tactcggaag aatcaaaaac gaagaagcgt
    26341 tctatgtttg gggtgttttt ttcctgaaaa taataataaa acctccgctt atccgctgct
    26401 gaaatgggca acagcaaata gtcccgctgc ccctgtagcc aaatttttac ggtccagaga
    26461 ccgctatcca tttcaatgcc acttgtggct atagcctcaa gatcttcatc agttttacga
    26521 cgctttttac ggaagtcgtt tatcaaccga tcactttccg ggaacactac atttagaggc
    26581 ttatccagag cattgcatat tgcctgtgcg agccccagct ttgcagcaac cttcccggtt
    26641 tcgatacgct gaatttgttg ctggctggtg ccgaccataa acgccagctc gcgctgggtg
    26701 atagatagct gggtacggag ctgtttgatg ttgttctgta acatggcatc ctccttcaga
    26761 aggagtagaa agtgccatta aaacacaaaa ttgagtataa acacataacc gtgtttatgc
    26821 tcactaatca acccccttga acgaaaagaa aatctcttca acgttcttct ctggtatgcc
    26881 agagatccac gatataaatg tcattgtcac gaagttcgta gtggacctca taatcatcaa
    26941 ataacacttt tcggacttcc cgaggttcat accgagtctg ctgtacacca attgccggat
    27001 gtgctgtaag accagcacaa ccagtaatca ggcgatccaa aacgtcatcg gcgtgctggc
    27061 ggctatactg aagggcgaaa cggtagatac gctcaaggtc atcctgagct ttaagcgtcc
    27121 agtaaatttc catttatcag gatttcttta agcgattagc aaaatccacc atgtccgagt
    27181 ggctcatcac acgaccggca tccacatcag caagaccggc ctggatgctc tggtgacgcc
    27241 gttctctttc ggccagcata ctggtcaacg cttccttaat aatccagctc ttagaacggt
    27301 ccaactcaat tgccagattc tcaacagcag cggccaattc aataggtatc tgtgcgctga
    27361 tggtttgttt agtaagcgaa cccataggga ttacctcgtt taataacatt taataactcc
    27421 tattaatatt agcatagagg tgcctataaa aacatcacat ctcatttcgc aggcaacatc
    27481 ccgggaaagt gcttcgccac gatatcattc atcttatcca ccagatccag tcgcttgaat
    27541 gtgatatgcc cactgccctt ctgaaagtag cgaatagtga acaactcatc ttcgaaaatc
    27601 tgctgatgcg gattatcgcg cacaaaatcc ataaaccgga tggagacatc atgccggtta
    27661 tcgggaatgg ccttcccatc cagcagatag aacatccgtt ccagatcggc aagctgatcg
    27721 cgccgccagc cccagttcag gctgtagccc catctgtcat gcttcaccag attgttaacg
    27781 ataatcttct taccgaaata acagggatta ttggttttgt agtcccagct caatccctta
    27841 aatacattga taatcccacg ttcgaaaacg tcctgcttat tgtggtgaag ttgttcaaag
    27901 gtactcagga tgttggcttc gctaatcgcc gggagatcgc cctcctccag gttctttgcc
    27961 cactgactgc gggcttcggc atccatcaga gtcaacatcc cggacttcag catgagatcg
    28021 cgccagatac tgcgatcgat attgcgggtg atcgccggca tcgctttgtc ggttggctgc
    28081 atcagccagc aatcaaaggc atatccatta cgcattgccc aggttccagc cacatcaccg
    28141 ccaatactgg atgtcaggct ggagatatca tgaagttttt ggattaacgt cgctatctgg
    28201 ttcagtgcac tatcacgacc cgtcacgata cgttcaatgc tggtcgagca aattagctcg
    28261 ttatgatcgg ttaagacttc agcttctgtt tgcataatct tttgtccgta taaaagcaaa
    28321 tgagccagcc atgaacagca ggcgcattat gaagggagtt atgagagaag taattttggc
    28381 gatgtaaatc gggaaggaga ctcgcagatt caccgtgccg tggtgttatg gcagcggcgc
    28441 agcagacctg tagcctgtcg cgcccgcaaa atatcgaccg cacgaagata aggcgtctgt
    28501 tcctgccagc taaatccttt cctgtcgata cggatcaatt cgtacttctc caccagaaaa
    28561 ttgacggcat cgaccagagt gattccggcg tctatgtgtt cctgaatcac cttttcattg
    28621 ttgaatggtg tgtcattaag attcagcccg tagtgctgct ccagcaggcg ggccagtaat
    28681 gtttgccaga ccacgacagg cgacagatat gacgtcgtcg cccgcagcgt cgttgcaggt
    28741 ataattttca taacgatgtt ccgtattaag gaggttaaaa ctcagcgttg agtgggataa
    28801 atagcgatat agacgtaacc acagcaaccc agcgtatcgg cttcacaggt tagtcctttg
    28861 gcgtatagcg tgacggtacg ttgttggcag ggggagagtt caccgtcagc cagtttcagc
    28921 tccagttgtt tcatcagcac cggaaaagcc tggtcgatat gcagcagatc cggggagctg
    28981 aatttcccgg tgattgcggc ccgatcagcc aggtaatgca ggttatgtct ttcctgcacc
    29041 agtcgagcag tgaagcgtgg tgtaacggta cagggaagcc cccattcttg ttgcatgacc
    29101 ttgtcctttt gcagcattac agccagcccc gttcggcaaa agagaggaca tcccccgccc
    29161 caataatgaa atgatccagc accctcactt tcaccaaatc cagtgtctta acaatattcg
    29221 ttgtaataag tctgtcggcc cgactgggtt ccgtcacgcc ggagggatgg ttatgcgcaa
    29281 ggatcactgc tgcggcgttg tgccgtagcg cagatttcac aatctcacgg ggatgtactt
    29341 ctgtgctgtt aatcgtgccg agaaatagcg tttcgtgttc cagcagtcgg ttctggttat
    29401 cgaggtagag cacaacaaac atttcccgct cctgtcgtgc cagatgcagt tgcagccagg
    29461 tttttgtggc gtcagaggaa agaaaagatt cgccaggatt gcgttgatat ttctccagca
    29521 gacgcatcgc acggcggata acacgttgtt cactcaccga aaataccggt gttagcatca
    29581 gttcagacat cgtgtttcct ttacttttaa tagcgttgtt tatgaggcca gttgcaacat
    29641 gttttcagcc atcacccaca gggcgcggtt gagtctgaca tcgccgtcga tgcctttaat
    29701 ggcgcgggtg tgcgtgcgac caccttttgc cgtgcgcccg tttaaaccac ctttgatcag
    29761 gttctcctga atacgctggt aggtcgtcca cagatcgtcg gattcatcct gccagcggcg
    29821 gggagccagc aactgggatt ccgtcaccgg ctggtgctct tcgccaaagc ggtaagtcag
    29881 cgccgccttt gccagcgcct gttgcgcagg gggaggcagc atcagcgact gcatggcatc
    29941 gcgtttctct tcaacctggt caaaaacact gaggacttca taagcgcctt caatcacttt
    30001 ctccaccaca ttgcctttat gcggtactcg tacttcacca aacgactcgc cgcagaccag
    30061 tccgttacaa caaacagaac ggaacagccc tggcagcatc tgataagaac tggaaccgtc
    30121 atggctgttg agcagaataa tttccggcac ctggttgcga ttgatctggt cttcccggcg
    30181 cagacgcagc atgtgttttg tatgctcacg tttacccaca tcacgaacac gggtctggca
    30241 ggcaaagaaa ggatgaaatc cttcacgctg gaggttatcg agcagggtaa tggtgggaat
    30301 ataggtatag cgttcgctgc gtgattcgtg cttattttcc ccgaatacgc tggggacata
    30361 ctgcatcaat tcgtcatgcg ttaatgggcg atcgcgacgg atttgattta cgcggccaaa
    30421 gcggctggct aaattcatgg taaatgcctt aataaagaat ataaaaaaca gggccgcagc
    30481 ttctgaaaaa ttgcggctaa tttaatgcgt gattaaaacg gacggtagta taggtgggat
    30541 aaaataacag gtacgtgcag atattaaatt acattccggc agtgcatata tgaaagtgat
    30601 atagtgctga aataatacat atctgaattc tcccggtgat agtcaccggg agaatttaag
    30661 tttgaaatga cacctcaacc acgggataat cttgtttcta ttccctgaac tctttcgtct
    30721 attaaccttt caataacaga tattttatct gacttgtaaa attcaaccag aaaatccaga
    30781 cgctctttat gcgtatcact gatagactcc cgttcccgtt tttcagaaag tttacgtctg
    30841 aagttttttt ggtaaaacgc tttatttaat ttctttaccg ctttctgctg gagatcatca
    30901 ttcgctgacc ataaatccag catggcataa acggcgtgat aggtttctac actattcagt
    30961 ggttccggga tctgaacggg gtccaactgg cttcctgtat tactaccgtg ctctttctgg
    31021 aatttattaa tgtaattata agcccatata cagacttcgt cattatcctc attaagccag
    31081 cgtaatttta ttctctgatg ttcatttgcc ctccagaata caagcctctc atatagccac
    31141 tgtctcatca acggtgcctg attagtctca atattaatat agtccagtaa tgcaacgatg
    31201 attgagtatc tttcgctatg gcttgcagga gaatctgtta ttccggcttt ctgataccag
    31261 ttaatgttta aatttgtgcc cagcttttcg ttaattgact ccatacagcg gtaatcaaga
    31321 cacatcacgc cccatagcca gaacgtcgct ctttcatttt cttttaacca actaaaatat
    31381 ttctccggca gaagcatcgt attaatcgac agctccagtg agcgaataaa attacttcgc
    31441 tgaatgggat cgggataccc ggcctcgagg gctgtattaa taattctctc cgaaagagat
    31501 gtatctggat tgttcccgtc aaacgcaaat tgatttgctc cctgaatgtt tttcctgatg
    31561 taccagaaga ggaacctgct ggttcggata tccttcactg ctattgatga gcgcacatta
    31621 acggccattg ttctacctcg tcatccttgt taaatcagtc gaaatcaata gcgtaattat
    31681 gatgtgtttt cgaagtaaaa ccgtagcaat aaatcaataa aagtggaaaa tatgtagtaa
    31741 tatcgtgtta ttccatagta atagagtacc attaacgtag ttttttattt attaccatac
    31801 tcacagatcc cgcctgtaga aaacggctgg atagtcttaa atttttccac gtatagccct
    31861 ggtggcttaa cgggtgaaac ctcccaggat aacgagttga catctggaag a
//
LOCUS       DADSYM010000041        31572 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2415
            SAMN08039579-rid11723173.denovo.041, whole genome shotgun sequence.
ACCESSION   DADSYM010000041 DADSYM010000000
VERSION     DADSYM010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039579
            Sequence Read Archive: SRR6362533
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 31572)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31572)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980162.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 03:39:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,484
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 4,347
            CDSs (with protein)               :: 4,347
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 43 of 85
            Pseudo Genes (incomplete)         :: 37 of 85
            Pseudo Genes (internal stop)      :: 19 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31572
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039579-rid11723173.denovo.041"
                     /strain="LGE2415"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8302 N 95.9628 W"
                     /collection_date="2015-08-24"
     gene            23..4579
                     /gene="sslE"
                     /locus_tag="J1Y83_001585"
     CDS             23..4579
                     /gene="sslE"
                     /locus_tag="J1Y83_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein metalloprotease SslE"
                     /protein_id="HBA7629740.1"
                     /translation="MNKKFKYKKSLLAAILSATLLAGCDGGGSGSSSDTPSVDSGSGT
                     LPEVKPDPTPTPEPTPEPTPDPEPTPDPTPDPEPTPEPEPEPVPTKTGYLTLGGSQRV
                     TGATCNGESSDGFTFTPGNTVSCVVGSTTIATFNTQSEAARSLRAVDKVSFSLEDAQE
                     LANSENKKTNAISLVTSSDSCPADAEQLCLTFSSVVDRARFEKLYKQIDLATDNFSKL
                     VNEEVENNAATDKAPSTHTSTVVPVTTEGTKPDLNASFVSANAEQFYQYQPTEIILSE
                     GQLVDSLGNGVAGVDYYTNSGRGVTDENGKFSFSWGETISFGIDTFELGSVRGNKSTI
                     ALTELGDEVRGANIDQLIHRYSTTGQNNTRVVPDDVRKVFAEYPNVINEIINLSLSNG
                     ATLDEGDQNVVLPNEFIEQFKTGQAKEIDTAICAKTNGCNEARWFSLTTRNVNDGQIQ
                     GVINKLWGVDTNYQSVSKFHVFHDSTNFYGSTGNARGQAVVNISNAAFPILMARNDKN
                     YWLAFGEKRAWDKNELAYITEAPSIVQPENVTRDTATFNLPFISLGQVGDGKLMVIGN
                     PHYNSILRCPNGYSWGGGVNSKGECTLSGDSDDMKHFMQNVLRYLSNDTWQPNTKSIM
                     TVGTNLENVYFKNHGQVTGSRAAFGFHPDFAGISVKHLTSYGDLNPEEIPLLILNGFE
                     YVTQVGSDPYAIPLRADTSKPKLTQQDVTDLIAYLNKGGSVLIMENVMSNLKEESASS
                     FVRLLDAAGLSMALNKSVVNNDPQGYPDRVRQQREKGIWVYERYPFVDGKPPYTIDET
                     TKEVIWKYQQDNKPDDKPKLEVASWLEDVDGKQVKRYAFIDEAEHETNESLEAAKAKI
                     IKAFPGLEECKDPTYHYEVNCLEYRPGTNVPVTGGMYVPRYTQLNLSADTAKAMVQAA
                     DLGTNIQRLYQHELYFRTNGRKGERLSSVDLERLYQNMSVWLWNEIEYSYDSSKEDEL
                     GFKTFTEFLNCYANDAYTKGTLCSAELKQSLIDNKMIYGEGSKAGMMNPSYPLNYMEK
                     PLTRLMLGRSWWDLNIKVDVEKYPGAVSVGGEEVTETISLYSNPTKWFAGNMQSTGLW
                     APAQKEVTIKSNANVPVTVTVALADDLTGREKHEVALNRPPRVTKTYSLDASGTVKFK
                     VPYGGLIYIKGNSSTNESASFTFTGVVKAPFYKDGAWKNDLNSPAPLGELESASFVYT
                     TPKKNLNASNYTGGLDQFAKDLDTFASSMNDFYGRNDEDGKHRMFTYKNLTGHKHRFT
                     NDVQISIGDAHSGYPVMNSSFSTNSTTLPTTPLNDWLIWHEVGHNAAETPLNVPGATE
                     VANNVLALYMQDRYLGKMNRVADDITVAPEYLEESNGQAWARGGAGDRLLMYAQLKEW
                     AEKNFDIKQWYPEGDLPKFYSDRKGMKGWNLFQLMHRKARGDDVSNDKFGGRNYCAES
                     NGNAADTLMLCASWVAQADLSEFFKKWNPGANAYQLPGASEMSFEGGVSQSAYNTLAA
                     MHLSKPEKGPETINKVTEYSMPAE"
     gene            4774..5583
                     /locus_tag="J1Y83_001586"
     CDS             4774..5583
                     /locus_tag="J1Y83_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417446.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HBA7629741.1"
                     /translation="MLFDVFQQYPAAMPILATAGGLIIGSFLNVVIWRYPIMLRQQMA
                     EFHGETPSTQSKISLALPRSHCPHCQQTIRVRDNIPLLSWLMLKGRCRDCQAKISKRY
                     PLVELLTALAFLLASLVWPESGWGLAVMILSAWLIAASIIDLDNQWLPDVFTQGVLWT
                     GLIAAWAQQSPLTLQDAVTGVLVGFITFYSLRWIAGIVLRKEALGMGDVLLFAALGGW
                     VGPLSLPNVALIASCCGLIYAVITKRGSTTLPFGPCLSLGGIATLYLQALF"
     gene            5649..6059
                     /gene="gspS2"
                     /locus_tag="J1Y83_001587"
     CDS             5649..6059
                     /gene="gspS2"
                     /locus_tag="J1Y83_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system pilot lipoprotein
                     GspS-beta"
                     /protein_id="HBA7629742.1"
                     /translation="MSIKQMPGRVLISLLLSVTGLLSGCASHNENASLLAKKQAQNIS
                     QNLPIKSAGYTLVLAQSSGTTVKMTIISESGTQTTQTPDAFLTSYQRQMCADPTVKLM
                     ITEGINYSITINDTRTGNQYQRKLDRTTCGIVKA"
     gene            6077..7036
                     /gene="gspC"
                     /locus_tag="J1Y83_001588"
     CDS             6077..7036
                     /gene="gspC"
                     /locus_tag="J1Y83_001588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspC"
                     /protein_id="HBA7629743.1"
                     /translation="MARVVFRDVRIYLIQLLTKIRHTLSQRQSLNTDKEHLRKIARAM
                     FWLMLLIISAKMAYSLWRYFSFSAEYTAVSASANKPLRADAKPFDKNDVQLVSQQNWF
                     GKYQPVAAPVKQPESAPVAETRLNVVLRGIAFGARPGAVIEEGGKQQVYLQGERLGSH
                     NAVIEEINRDHVMLRYQGKMERLSLAEEERPPVAVTSKKAASDEAKQAVAEPAASAPV
                     ELPAAVRQALAKDPQKIFNYIQLTPVRKEGIVGYAVKPGADRSLFDASGFKEGDIAIA
                     LNQQDFTDPRAMIALMRQLPSMDSIQLTVLRKGARYDISIALR"
     gene            7066..9126
                     /gene="gspD"
                     /locus_tag="J1Y83_001589"
     CDS             7066..9126
                     /gene="gspD"
                     /locus_tag="J1Y83_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020234996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system secretin GspD"
                     /protein_id="HBA7629744.1"
                     /translation="MFWRDMTLSIWRKKTTGLKTKKRLLSLMLAAALCSSPVWAEEAT
                     FTANFKDTDLKSFIETVGANLNKTIIMGPGVQGKVSIRTMTPLNERQYYQLFLNLLEA
                     QGYAVVPMENDVLKVVKSSAAKVEPLPLVGEGSDNYAGDEMVTKVVPVRNVSVRELAP
                     ILRQMIDSAGSGNVVNYDPSNVIMLTGRASVVERLTEVIQRVDHAGNRTEEVIPLDNA
                     SASEIARVLESLTKNSGENQPATLKSQIVADERTNSVIVSGDPATRDKMRRLIRRLDS
                     EMERSGNSQVFYLKYSKAEDLVDVLKQVSGTLTAAKEEAEGTVGSGREVVSIAASKHS
                     NALIVTAPQDIMQSLQSVIEQLDIRRAQVHVEALIVEVAEGSNINFGVQWGSKDAGLM
                     QFANGTQIPIGTLGAAISAAKPQKGSTVISENGATTINPDTNGDLSTLAQLLSGFSGT
                     AVGVVKGDWMALVQAVKNDSSSNVLSTPSITTLDNQEAFFMVGQDVPVLTGSTVGSNN
                     SNPFNTVERKKVGIMLKVTPQINEGNAVQMVIEQEVSKVEGQTSLDVVFGERKLKTTV
                     LANDGELIVLGGLMDDQAGESVAKVPLLGDIPLIGNLFKSTADKKEKRNLMVFIRPTI
                     LRDGMAADGVSQRKYNYMRAEQIYRDEQGLSLMPHTAQPILPAQNQALPPEVRAFLNA
                     GRTR"
     gene            9126..10619
                     /gene="gspE"
                     /locus_tag="J1Y83_001590"
     CDS             9126..10619
                     /gene="gspE"
                     /locus_tag="J1Y83_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001525635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system ATPase GspE"
                     /protein_id="HBA7629745.1"
                     /translation="MVPVAQETTANTVRLPYSFSRRFSLVAWCEASLEILHVHPLSLS
                     VLQELQRGLNAPFTLRQIDEAEFEQRLNAVWQRDSSEARQLMEDLGSAEDFFTLAEEL
                     PETEDLLESDDDAPIIKLINAMLAEAIKEGASDIHIETFEKSLVIRFRVDGTLHEMLR
                     PGRKLASLLVSRIKVMARLDIAEKRVPQDGRIALLLGGRAIDVRVSTMPSAWGERVVL
                     RLLDKNQARLTLERLGLSLELTAQLRQLLHKPHGIFLVTGPTGSGKSTTLYAGLQELN
                     NHSRNILTVEDPIEYMIEGIGQTQVNTRVGMTFARGLRAILRQDPDVVMVGEIRDTET
                     AEIAVQASLTGHLVLSTLHTNTAVGAITRLQDMGVEPFLLSSSLTGVMAQRLVRTLCS
                     DCRQAAPATDEEKRLMGISDTHAVTLYHPQGCPACNHKGFRGRTAIHELIVVDATLRD
                     LIHRQAGELELERYVRQHSAGIRSNGIEKVLAGETSLDEVLRVTMEA"
     gene            10619..11842
                     /gene="gspF"
                     /locus_tag="J1Y83_001591"
     CDS             10619..11842
                     /gene="gspF"
                     /locus_tag="J1Y83_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001173399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system inner membrane protein
                     GspF"
                     /protein_id="HBA7629746.1"
                     /translation="MALFYYQALERNGRKTKGMIEADSARHARQLLRGKDLIPVHIEA
                     RMNASAGGLLQRRRHAHRRVAAADLALFTRQLATLVQAAMPLETCLQAVSEQSEKLHV
                     KSLGMALRSRIQEGYTLSDSLREHPRVFDSLFCSMVAAGEKSGHLDVVLNRLADYTEQ
                     RQRLKSRLLQAMLYPLVLLVVATGVVTILLTAVVPKIIEQFDHLGHALPASTRMLIAM
                     SDALQASGVYWLAGLLGLLVLGQRLLKNPAMRLRWDKTLLRLPVTGRVARGLNMARFS
                     RTLSILTASSVPLLEGIQTAAAVSANRYVEQQLLLAADRVREGSSLRAALADLRLFPP
                     MMLYMIASGEQSGELETMLEQAAVNQEREFDTQVGLALGLFEPALVVMMAGVVLFIVI
                     AILEPMLQLNNMVGM"
     gene            11859..12314
                     /gene="gspG"
                     /locus_tag="J1Y83_001592"
     CDS             11859..12314
                     /gene="gspG"
                     /locus_tag="J1Y83_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003912123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system major pseudopilin GspG"
                     /protein_id="HBA7629747.1"
                     /translation="MNSLSRTQKPRAGFTLLEVMVVIVILGVLASLVVPNLLGNKEKA
                     DRQKAISDIVALENALDMYRLDNGRYPTTEQGLEALIQQPANMADARNYRTGGYIKRL
                     PKDPWGNDYQYLSPGEKGLFDVYTLGADGQENGEGAGADIGNWNLQEFQ"
     gene            12318..12881
                     /gene="gspH"
                     /locus_tag="J1Y83_001593"
     CDS             12318..12881
                     /gene="gspH"
                     /locus_tag="J1Y83_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001394829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspH"
                     /protein_id="HBA7629748.1"
                     /translation="MPERGFTLLEIMLVIFLIGLASAGVVQTFATASEPPAKKAAQAF
                     LTRFAQFKDRAVIEGQTLGVLIDPPGYQFMQRRQGQWLPVSATRLSAQVTVPKQVQML
                     LQPGSDIWQKEYALEMQRRRLTLHDIELELQKEAKKKTPQIRFSPFEPATPFTLRFYS
                     AAQNACWAVKLAHDGALSLNQCDERMP"
     gene            12878..13249
                     /gene="gspI"
                     /locus_tag="J1Y83_001594"
     CDS             12878..13249
                     /gene="gspI"
                     /locus_tag="J1Y83_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001709020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspI"
                     /protein_id="HBA7629749.1"
                     /translation="MKRGFTLLEVMLALAIFALAAMAVLQIASGALSNQHVLEEKTVA
                     GWVAENQTAQLYLMTREQRAVRHQGESDMAGSRWYWRTTPLNTGNVLLQAVDIEVSLH
                     DDFSPVIQSRRAWFSAVGGQQ"
     gene            13246..13851
                     /gene="gspJ"
                     /locus_tag="J1Y83_001595"
     CDS             13246..13851
                     /gene="gspJ"
                     /locus_tag="J1Y83_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001255030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspJ"
                     /protein_id="HBA7629750.1"
                     /translation="MRRARAGFTLLEMLVAIAIFASLALMAQQVTNGVTRVNSAVAGH
                     DQKLNLMQQTMSFLNHDLTQMMPRPVRGDQGQREPALLAGAGVLASESEGMRFVRGGV
                     VNPLMRLPRSNLLTVGYRIHGGYLERLAWPLTDAAGSVKPTTQKLIPADSLRLQFYDG
                     TRWQESWSSVQAIPVAVRITLHSPQWGEIERIWLLRGPQLS"
     gene            13848..14825
                     /gene="gspK"
                     /locus_tag="J1Y83_001596"
     CDS             13848..14825
                     /gene="gspK"
                     /locus_tag="J1Y83_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001554494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspK"
                     /protein_id="HBA7629751.1"
                     /translation="MITLPPKRGMALVVVLVLLAVMMLVTITLSGRMQQQLGRTRSQQ
                     EYQQALWYSASAESLALSALSLSLKNEKRVHLAQPWASGPRFFPLPQGQIAVTLRDAQ
                     ACFNLNALAQPTTASRPLAVQQLIALISRLDVPAYRAELIAESLWEFIDEDRSVQTRL
                     GREDSEYLARSVPFYAANQPLADISEMRVVQGMDAGLYQKLKPLVCALPMARQQININ
                     TLDVTQSVILEALFDPWLSPVQARALLQQRPAKGWEDVDQFLAQPLLADVDERTKKQL
                     KTVLSVDSNYFWLRSDITVNEIELTMNSLIVRMGPQHFSVLWHQTGESE"
     gene            14822..16000
                     /gene="gspL"
                     /locus_tag="J1Y83_001597"
     CDS             14822..16000
                     /gene="gspL"
                     /locus_tag="J1Y83_001597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000097227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspL"
                     /protein_id="HBA7629752.1"
                     /translation="MSSMLEIFFPLCAADPIRWQRHTPDVEHGIWPDVADERLQQWLQ
                     TDAIRLYIPGEWISVWQVELPDVPRKQIPTILPALLEEELNQDIDELHFAPLKIDQQL
                     ATVAVIHQQHMRNIAQWLQANGITRATVAPDWMSIPCGFMAGDAQRVICRIDECRGWS
                     AGRALAPVMFRAQLNEQDIPLSLTVVGIAPEELSAWAGADAERLTVTALPAIITYGEP
                     EGNLLTGPWQPRVSYRKQWARWRVMILPILLILVALVVERGVTLWSVSEQVAQSRTQA
                     EKQFLTLFPEQKRIVNLRSQVTMALKKYRPQADDTDLLAELSAIASTLKSASLSDIEM
                     RGFTFDQKRQTLHLQLRAANFASFDKLRSALAADYVVQQDALQKEGDAVSGGVTLRRK
                     "
     gene            16002..16538
                     /locus_tag="J1Y83_001598"
     CDS             16002..16538
                     /locus_tag="J1Y83_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HBA7629753.1"
                     /translation="MLRDKFILYFQQWRERQLSRGEHWLTQHLAGRSPREKGMLLAAV
                     VFLFSAGYYVLIWQPLSERIEQQETMLQQLVAMNARLKSAAPDIIAARKSGTTTPAQV
                     SRVISDSASAHSVAIKRIAERGENIQVWIDPVVFNDLLNWLKALDEKYALRVTQIDVS
                     AAEKPGMVNVQRLEFGRG"
     gene            complement(16686..16761)
                     /locus_tag="J1Y83_001599"
     tRNA            complement(16686..16761)
                     /locus_tag="J1Y83_001599"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(16726..16728),aa:Phe,seq:gaa)
     gene            complement(16867..17574)
                     /locus_tag="J1Y83_001600"
     CDS             complement(16867..17574)
                     /locus_tag="J1Y83_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HBA7629754.1"
                     /translation="MVIGPFINASAVLLGGVFGALLSQRLPERIRVSMTSIFGLASLG
                     IGILLVVKCANLPAMVLATLLGALIGEICLLEKGVNTVVAKAHNLFHHSRKKPAHESF
                     IQNFVAIIVLFCASGTGIFGAMNEGMTGDPSILIAKSFLDFFTAMIFACSLGIAVSVI
                     SIPLLIIQLTLAWAAALILPLTTPSMMADFSAVGGLLLLATGLRICGIKMFPVVNMLP
                     ALLLAMPLSAAWTAWFA"
     gene            17973..20108
                     /locus_tag="J1Y83_001601"
     CDS             17973..20108
                     /locus_tag="J1Y83_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine decarboxylase"
                     /protein_id="HBA7629755.1"
                     /translation="MKSMNIAASSELVSRLSTHRCVVALGDTDFTDVAAVVITAADSR
                     SGILALLKRTGFHLPVFLYSEHAVELPAGVTAVINGNEQQWLELESAACQYEENLLPP
                     FYDTLTQYVEMGNSTFACPGHQHGAFFKKHPAGRHFYDFFGENIFRADMCNADVKLGD
                     LLIHEGSAKDAQKFAAKVFHADKTYFVLNGTSAANKVVTNALLTRGDLVLFDRNNHKS
                     NHHGALIQAGATPVYLEASRNPFGFIGGIDAHCFNEEYLRQQIRDVAPEKADLPRPFR
                     LAIIQLGTYDGTVYNARQVIDTVGHLCDYILFDSAWVGYEQFIPMMADSSPLLLELNE
                     NDPGIFVTQSVHKQQAGFSQTSQIHKKDNHIRGQARFCPHKRLNNAFMLHASTSPFYP
                     LFAAMDVNAKIHEGESGRRLWAECVELGIESRKAILARCKLFRPFIPPVVDGKLWQDY
                     PTSVLASDRRFFSFEPGAKWHGFEGYAADQYFVDPCKLLLTTPGIDAETGEYSDFGVP
                     ATILAHYLRENGIVPEKCDLNSILFLLTPAESHEKLAQLVAMLAQFEQHIEDDSPLAE
                     VLPSVYNKYPVRYRDYTLRQLCQEMHDLYVSFDVKDLQKAMFRQQSFPSVVMNPQDAH
                     SAYIRGEVELVRIRDAEGRIAAEGALPYPPGVLCVVPGEVWGGAVQRYFLALEEGVNL
                     LPGFSPELQGVYSETDADGMKRLYGYVLK"
     gene            complement(20157..21413)
                     /locus_tag="J1Y83_001602"
     CDS             complement(20157..21413)
                     /locus_tag="J1Y83_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease"
                     /protein_id="HBA7629756.1"
                     /translation="MNLKLQLKILSFLQFCLWGSWLTTLGSYMFVTLKFDGASIGAVY
                     SSLGIAAVFMPALLGIVADKWLSAKWVYAICHTIGAITLFMAAEVTTPEAMFLVILIN
                     SFAYMPTLGLINTISYYRLQNAGMDIVTDFPPIRIWGTIGFIMAMWVVSLSGFELSHM
                     QLYIGAALSAILVLFTLTLPHIPVAKQQANQSWTTLLGLDAFALFKNKRMAIFFIFSM
                     LLGAELQITNMFGNTFLHSFDKDPMFASSFIVQHASIIMSISQISETLFILTIPFFLS
                     RYGIKNVMMISIVAWILRFALFAYGDPTPFGTVLLVLSMIVYGCAFDFFNISGSVFVE
                     KEVSPAIRASAQGMFLMMTNGFGCILGGIVSGKVVEMYTQNGITDWQTVWLIFAGYSV
                     VLAFAFMAMFKYKHVRVPTGTQTVSH"
     gene            complement(21615..22694)
                     /gene="mltC"
                     /locus_tag="J1Y83_001603"
     CDS             complement(21615..22694)
                     /gene="mltC"
                     /locus_tag="J1Y83_001603"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417438.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     MltC"
                     /protein_id="HBA7629757.1"
                     /translation="MKKYLALALIAPLLISCSTTKKDDTYNEAWVKDTNGFDILMGQF
                     AHNIENIWGFKEVVIAGPKDYVKYTDQYQTRSHINFDDGTITIETIAGTEPAAHLRRA
                     IIKTLLMGDDPSSVDLYSDVDDITISKEPFLYGQVVDNTGQPIRWEGRASNFADYLLK
                     NRLKSRSNGLRIIYSVTINMVPNHLDKRAHKYLGMVRQASRKYGVDESLILAIMQTES
                     SFNPYAVSRSDALGLMQVVQHTAGKDVFRSQGKSGTPSRSFLFDPASNIDTGTAYLAM
                     LNNVYLGGIDNPTSRRYAVITAYNGGAGSVLRVFSNDKIQAANIINTMTPGDVYQTLT
                     TRHPSAESRRYLYKVNTAQKSYRRR"
     gene            complement(22758..23033)
                     /locus_tag="J1Y83_001604"
     CDS             complement(22758..23033)
                     /locus_tag="J1Y83_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative damage protection protein"
                     /protein_id="HBA7629758.1"
                     /translation="MSRTIFCTFLQREAEGQDFQLYPGELGKRIYNEISKEAWAQWQH
                     KQTMLINEKKLNMMNAEHRKLLEQEMVNFLFEGKEVHIEGYTPEDKK"
     gene            complement(23061..24113)
                     /gene="mutY"
                     /locus_tag="J1Y83_001605"
     CDS             complement(23061..24113)
                     /gene="mutY"
                     /locus_tag="J1Y83_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HBA7629759.1"
                     /translation="MQASQFSAQVLDWYDKYGRKTLPWQIDKTPYKVWLSEVMLQQTQ
                     VATVIPYFERFMARFPTVTDLANAPLDEVLHLWTGLGYYARARNLHKAAQQVAALHGG
                     KFPETFEEVAALPGVGRSTAGAILSLSLGKHFPILDGNVKRVLARCYAVSGWPGKKEV
                     ENKLWSLSEQVTPAVGVERFNQAMMDLGAMICTRSKPKCSLCPLQNGCIAAANNSWSL
                     YPGKKPKQTLPERTGYFLLLQHEDEVLLAQRPPSGLWGGLYCFPQFADEESLRQWLAQ
                     RQIAADNLTQLTAFRHTFSHFHLDIVPMWLPVSSFIGCMDEDNALWYNLAQPPSVGLA
                     APVERLLQQLRTGAPV"
     gene            24274..24993
                     /gene="trmB"
                     /locus_tag="J1Y83_001606"
     CDS             24274..24993
                     /gene="trmB"
                     /locus_tag="J1Y83_001606"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HBA7629760.1"
                     /translation="MKNDVISPEFDENGRPLRRIRSFVRRQGRLTKGQEHALENYWPV
                     MGVEFSEDMLDFPVLFGREAPVTLEIGFGMGASLVAMAKDRPEQDFLGIEVHSPGVGA
                     CLSSAHEEGLSNLRVMCHDAVEVLHKMIPDNSLRMVQLFFPDPWHKARHNKRRIVQVP
                     FAELVKSKLQLGGIFHMATDWEPYAEHMLEVMSSIDGYKNLSESNDYVPRPASRPVTK
                     FEQRGHRLGHGVWDLMFERVK"
     gene            24993..25319
                     /locus_tag="J1Y83_001607"
     CDS             24993..25319
                     /locus_tag="J1Y83_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF469 domain-containing protein"
                     /protein_id="HBA7629761.1"
                     /translation="MAKNRSRRLRKKMHIDEFQELGFSVAWRFPEGTSEEQIDKTVDD
                     FINEVIEPNKLAFDGSGYLAWEGLICMQEIGKCTEEHQAIVRKWLEERKLEEVRTSEL
                     FDVWWD"
     gene            25503..26222
                     /locus_tag="J1Y83_001608"
     CDS             25503..26222
                     /locus_tag="J1Y83_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2884 domain-containing protein"
                     /protein_id="HBA7629762.1"
                     /translation="MMRKMLLAAALSVTAMTAHADYQCSVTPRDDVIVSPQTVQVKGE
                     NGNLVITPDGNVMYNGKQYSLNAAQREQAKDYQAELRSTLPWIDEGAKSRVEKARIAL
                     DKIIVQEMGESSKMRSRLTKLDAQLKEQMNRIIETRSDGLTFHYKAIDQVRAEGQQLV
                     NQAMGGILQDSINEMGAKAVLKSGGNPLQNVLGSLGGLQSSIQTEWKKQEKDFQQFGK
                     DVCSRVVTLEDSRKALVGNLK"
     gene            26398..27444
                     /gene="ansB"
                     /locus_tag="J1Y83_001609"
     CDS             26398..27444
                     /gene="ansB"
                     /locus_tag="J1Y83_001609"
                     /EC_number="3.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-asparaginase 2"
                     /protein_id="HBA7629763.1"
                     /translation="MEFFKKTALAALVMGFSGAALALPNITILATGGTIAGGGDSATK
                     SNYTAGKVGVENLVNAVPQLKDIANVKGEQVVNIGSQDMNDDVWLTLAKKINTDCDKT
                     DGFVITHGTDTMEETAYFLDLTVKCDKPVVMVGAMRPSTSMSADGPFNLYNAVVTAAD
                     KASANRGVLVVMNDTVLDGRDVTKTNTTDVATFKSVNYGPLGYIHNGKIDYQRTPARK
                     HTSDTPFDVSKLNELPKVGIVYNYANASDLPAKALVDAGYDGIVSAGVGNGNLYKTVF
                     DTLATAAKNGTAVVRSSRVPTGATTQDAEVDDAKYGFVASGTLNPQKARVLLQLALTQ
                     TKDPQQIQQIFNQY"
     gene            27561..28379
                     /locus_tag="J1Y83_001610"
     CDS             27561..28379
                     /locus_tag="J1Y83_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000745241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1202 family protein"
                     /protein_id="HBA7629764.1"
                     /translation="MKKQWIVGTALFMLMTGNVRADGEPPTENILKDQFKKQYHGILK
                     LDVITLKNLDAKGNQATWSAEGDVSSSDDLYTWVGQLADYELLEQTWTKDKPVKFSAM
                     LTSKGTPASGWSVNFYSFQAAASDRGRVVDDIKTNNKYLIVNSEDFNYRFSQLESALN
                     NQNNSIPALKKDVKALDKQMVAAQKAADAYWGKDANGKQMTREDAFKKIHQQRDDFNK
                     QNDSEAFAVKYDKEVYQPAIAACHKQSEECYEVPIQQKRDFDINEQRRQTFLQS"
     gene            28395..28568
                     /locus_tag="J1Y83_001611"
     CDS             28395..28568
                     /locus_tag="J1Y83_001611"
                     /inference="COORDINATES: protein motif:HMM:NF018429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1202 family protein"
                     /protein_id="HBA7629765.1"
                     /translation="MQDDWITLEKGQYPLTMKVSEINSKKVTILMKIDDINQANERWK
                     KDTEQLRRNGVIK"
     gene            complement(28624..29925)
                     /locus_tag="J1Y83_001612"
     CDS             complement(28624..29925)
                     /locus_tag="J1Y83_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708726.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HBA7629766.1"
                     /translation="MDSYIALTLFGCFFVLVFLGVPISFSIGIATVASMLLMFPWDIA
                     AITVSQRLANGLDNFALLAIPFFIFAGTLMNSGGIAIRLINLAKVMVGRVPGSLGHVN
                     VLANMMFGSISGSAVAAAAAVGGTLNPIQTKEGYDPTFSTAVNVSSCITGLLIPPSNV
                     LIVFSLTAGGVSVASLFMAGYLPGILMGLAVMVVCAIIAKRRGYPLSERATFAQACKA
                     FLDALPSLLLVFIVMGGILGGVFTATEASAIAVVYTFMLSVLIYREVKWRDLPKLILE
                     SVVTTSIVLLLIGFSVGMSWAMTNADIPYMISDALMGISDNPLIILLLINIVLLIVGI
                     FMDMTPAVLIFTPIFLPIAQELGMDPVHFGIMMVANLCIGLLTPPVGSALFVGCSISG
                     VKIQHLIKPLLPFYAALLVALMMITYIPQISLFIPQLLGLM"
     gene            complement(29925..30428)
                     /locus_tag="J1Y83_001613"
     CDS             complement(29925..30428)
                     /locus_tag="J1Y83_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708723.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HBA7629767.1"
                     /translation="MIFNRLKLAVDRVIAAFSVAVMLALVVCVVWQVFSRYILNHPST
                     LTDELARFLMIWVGLLGAAYTVGAQRHLSIDLFALALNKRKQLLLSIVINFLILGFAG
                     SVIVTGGLKLIDKTLATSQVSAAMQIPMGYVYIILPLSGVVMMFYALCFINQSIQQLK
                     QPAQEAS"
     gene            complement(30473..31459)
                     /locus_tag="J1Y83_001614"
                     /pseudo
     CDS             complement(30473..31459)
                     /locus_tag="J1Y83_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014839537.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
BASE COUNT         7961 a         7744 c         8531 g         7336 t
ORIGIN      
        1 tgttttgtca cttgcgttat taatgaataa gaaatttaaa tataagaaat cgcttttagc
       61 ggctatttta agcgcaaccc tgttagccgg ttgtgatggt ggtggttcag gatcgtcctc
      121 cgatacgccg tctgtagatt ctggatcagg gactttgccg gaagtgaaac ccgatccaac
      181 accaaccccg gagccgacac ctgagccgac gccggaccca gaacctacgc cggatccaac
      241 acctgatcct gagccgacac cagaaccgga gccagaacct gttcctacga aaacgggtta
      301 tctgaccctg ggcggaagcc agcgggtaac tggtgctacc tgtaatggtg aatccagcga
      361 tggctttacc tttacgccag gcaataccgt gagttgtgtg gtgggcagta cgaccattgc
      421 aacattcaac acccagtcag aagctgcgcg tagcctgcgt gcggttgaca aagtgtcgtt
      481 tagcctggag gacgcgcagg agctggcgaa ttctgaaaat aagaaaacca acgccatctc
      541 tctggtgacg tccagcgaca gttgccccgc agatgcagaa cagctttgtc ttactttctc
      601 gtcagtggtt gatcgcgcgc gatttgaaaa actgtataag caaattgatc tggcaacaga
      661 caatttcagc aagctggtca atgaagaggt ggaaaacaat gctgcgactg ataaagcgcc
      721 gtccacccat acctcaacgg tagtgccagt cacgactgag ggaacaaaac cggatctgaa
      781 cgcgtccttc gtgtcggcta acgcggaaca gttttatcag tatcaaccca ctgaaatcat
      841 tctttccgaa ggccaactgg tggatagcct ggggaacggt gttgctggcg ttgactacta
      901 caccaattca ggccgtggcg taactgacga aaacggtaaa ttctccttta gctggggcga
      961 aaccatctcc tttggtatcg atacctttga actgggctca gtacgtggca ataagtcgac
     1021 cattgcgctg actgaattgg gtgatgaagt tcgcggggca aatatcgatc agctcattca
     1081 tcgttattcg acgactggtc aaaataatac ccgtgttgtt ccggacgatg tacgcaaggt
     1141 ctttgccgaa tatcccaacg tgatcaacga gataatcaat ctttcgttat ccaacggtgc
     1201 gacgctggat gaaggcgatc aaaacgttgt gctgcctaac gaatttatcg agcagtttaa
     1261 gacgggtcag gccaaagaga tcgataccgc gatttgtgcg aaaaccaacg gttgcaacga
     1321 ggctcgctgg ttctcgctga caacgcgcaa tgttaatgac ggccagattc agggcgttat
     1381 taacaagctg tggggcgtgg atacgaacta tcagtctgtc agcaagttcc acgtcttcca
     1441 tgactctacc aacttctatg gcagcaccgg taacgcgcgc ggtcaggcgg tggtaaatat
     1501 ctccaacgcg gcattcccga ttctgatggc gcgtaatgat aaaaactact ggctggcctt
     1561 cggtgaaaaa cgcgcctggg ataaaaatga gctggcgtac attacggaag cgccttccat
     1621 tgtgcagcca gagaacgtta cgcgcgatac cgcgaccttc aacctgccgt ttatttcgct
     1681 ggggcaagtc ggcgacggca aactgatggt tatcggtaac ccgcactaca acagcatttt
     1741 gcgttgccct aatggttaca gttggggcgg tggtgttaat agtaaaggtg agtgtacgct
     1801 cagcggtgat tctgatgaca tgaagcactt tatgcagaac gtactgcgct acttgtcaaa
     1861 tgacacctgg cagccaaata ccaagagcat catgactgtc ggcaccaacc tggagaacgt
     1921 ttatttcaaa aatcatggcc aggttacggg gagtcgtgct gcgtttgggt tccatccgga
     1981 ttttgccggt atctctgtta agcatttgac cagctatggc gatctgaatc cggaagagat
     2041 tccgttgctg attctcaacg gctttgaata tgtcactcag gtaggtagcg atccttatgc
     2101 aatcccactg cgagcagaca ccagcaaacc gaagctgact cagcaggatg tgaccgatct
     2161 gatcgcttat ctgaacaaag gtggctcggt gctgatcatg gaaaacgtga tgagcaatct
     2221 taaggaagag agcgcgtcca gttttgtgcg tctgctggat gccgcgggtc tgtcaatggc
     2281 tctgaacaaa tcggtggtga acaacgatcc gcaagggtat ccggatcgcg ttcgtcagca
     2341 gcgcgaaaaa ggtatctggg tatatgaacg ttatccgttt gttgatggaa aaccgccgta
     2401 taccattgat gaaacaacga aagaagttat ctggaaatac cagcaagaca acaagcctga
     2461 tgacaagccg aaactggaag tggccagctg gctggaagat gtcgatggca aacaggtcaa
     2521 gcgctatgcc tttattgatg aggcggagca tgaaacaaac gagtccttgg aggcggcaaa
     2581 ggcaaaaatc attaaggcat ttcctggatt agaggagtgt aaggacccga cataccacta
     2641 cgaggtcaac tgtctggaat accgtcctgg caccaatgtt ccggtaaccg ggggaatgta
     2701 tgttccgcgc tatacgcaac tgaatctcag cgccgacact gcgaaagcga tggtgcaggc
     2761 tgcggattta ggcaccaaca ttcagcgtct gtatcagcat gagctctact tccgaaccaa
     2821 tggtcgcaaa ggtgagcgtc tgagcagcgt cgatctggaa cgtctgtacc agaacatgtc
     2881 ggtctggctg tggaatgaaa ttgaatacag ctatgacagc agcaaggaag acgagctggg
     2941 ctttaaaacg ttcaccgagt tcctgaactg ctacgccaac gatgcttaca ccaaaggtac
     3001 gctgtgctcc gcagagctga aacaatcgct gatcgataac aagatgatct acggtgaagg
     3061 cagcaaagcg ggcatgatga acccgagcta tccactcaac tatatggaaa aaccgctgac
     3121 gcgcctgatg ctgggccgtt cctggtggga tctgaacatt aaggttgatg tggagaagta
     3181 ccctggagcg gtatctgtag ggggagaaga ggttactgaa accatcagcc tgtactcgaa
     3241 tccgaccaaa tggtttgcag gtaacatgca gtcaactggc ctgtgggcac cggctcagaa
     3301 agaggtcacc attaagtcca atgcgaacgt tcctgtgacc gtcaccgtgg cgctggctga
     3361 cgacctgacc ggacgtgaga agcatgaagt tgcgctgaac cgtccgccaa gagtgactaa
     3421 aacgtactct ctggacgcta gcggtacggt gaagttcaag gtgccttacg gtggcctgat
     3481 ttatatcaag ggcaatagct ctaccaatga atctgccagc ttcaccttta ctggcgtggt
     3541 aaaagcaccg ttctataaag acggcgcatg gaaaaacgat ctgaactctc ctgccccgct
     3601 gggcgaactg gagtctgcgt cgttcgtcta taccacaccg aagaagaacc tgaatgccag
     3661 caattacacg ggcggactgg atcaattcgc taaagatctg gatacctttg ccagctcgat
     3721 gaatgatttc tacggtcgta atgatgaaga cggtaagcac cggatgttta cctataaaaa
     3781 cttgacgggc cacaagcatc gtttcacaaa cgatgtgcag atctccatcg gtgatgcgca
     3841 ctctggttat ccggtaatga acagcagctt ctcgacgaac agcaccacgc tgccgacgac
     3901 gccgctgaac gactggctga tttggcacga agtcggtcat aacgctgcag aaacaccgct
     3961 gaacgtaccg ggtgcaactg aagtggcgaa caacgtgctg gcgctgtaca tgcaggatcg
     4021 ctatctcggc aagatgaacc gtgtcgctga cgacattacc gtcgcgccgg aatatctgga
     4081 ggagagcaac ggtcaggcat gggcgcgcgg cggtgcgggt gaccgtctgc tgatgtacgc
     4141 gcagctgaaa gagtgggcag agaaaaactt tgatatcaaa cagtggtatc cagaaggtga
     4201 cctgcctaag ttctacagcg atcgtaaagg gatgaagggc tggaacctgt tccagttgat
     4261 gcaccgtaaa gcgcgcggcg atgatgtcag caatgacaag tttggcggca gaaattactg
     4321 tgctgagtca aacggtaacg ctgctgacac gctgatgctg tgtgcatcct gggtcgctca
     4381 ggcggatctt tcggaattct ttaagaaatg gaatccgggc gcaaatgctt accagcttcc
     4441 gggggcaagt gagatgagct tcgaaggcgg agtgagccag tcggcttaca acacgctcgc
     4501 ggcgatgcac ctgtcgaaac cggaaaaagg gccggaaacc attaataagg ttaccgagta
     4561 ttcgatgcca gccgaataag cctgtaggcc ggataaggcg ttcacgccgc atccggcatg
     4621 aaataaggcg caccgggtca acatatatgc ccgatgcgcc agcttatcgg gcctggaggg
     4681 aagaagccgc gattacttgc gttggcagac acctgtaggc cggataaggc gttcacgccg
     4741 catccggcaa attacaacaa acaaccttta accatgcttt ttgatgtttt tcagcaatac
     4801 cccgcggcga tgcccatact ggcaaccgca ggaggattga tcatcggcag ttttttgaat
     4861 gtggtgattt ggcgttaccc catcatgctg cgccaacaaa tggcggagtt tcatggtgaa
     4921 acgccgagta cgcagtcaaa aataagcctt gcgctaccgc gttcgcactg tccgcattgc
     4981 cagcagacta tccgcgttcg tgacaatatt ccgctgctct catggttgat gctcaaaggg
     5041 cgctgccgcg actgtcaggc gaaaatcagc aagcgttatc cgctggttga gttattgaca
     5101 gcacttgctt ttttgctggc gagtctggtg tggccagaaa gtggatgggg actggcggtg
     5161 atgatattat ccgcgtggct gattgccgcg agcatcatcg acctcgacaa ccaatggctg
     5221 cccgatgttt ttactcaggg cgtattgtgg acaggactga ttgcggcatg ggcgcagcag
     5281 agtccgttaa cgctacaaga cgcagtcacc ggcgttctgg tggggtttat tacgttttac
     5341 tccctgcgct ggatagccgg aatagttctg cgtaaagaag cattaggcat gggcgatgta
     5401 ttactcttcg ctgcgttagg tggctgggtg gggccgttgt cgttacccaa tgtggcttta
     5461 atcgcatcat gctgcggcct gatatatgcc gttattacaa aaagaggatc aaccacactg
     5521 ccttttggac cgtgtttaag tctgggcggt atagcaacac tttatctaca ggcattgttt
     5581 taatgataac cacgtcatta tcaaagtgac attttaactc ttattaataa ccttagagat
     5641 tatttaccat gtcgataaaa cagatgccag ggagggtatt aatatcgcta ttgttgagcg
     5701 ttacaggatt attaagtggc tgcgccagcc ataatgaaaa cgccagttta ctggcgaaaa
     5761 aacaggcgca aaatatcagc caaaacctgc cgattaaatc tgcgggatat accttagtgc
     5821 tggcgcaaag tagtggcacg acggtaaaaa tgaccattat cagcgaatcg ggtactcaga
     5881 ccacgcagac acctgacgcc tttttaacca gctatcaacg acaaatgtgc gctgacccaa
     5941 cggtgaaatt aatgatcacc gagggaatta attacagcat aacgattaat gatacacgta
     6001 caggtaacca gtatcagcgg aaactggatc gtaccacctg tggaatagtc aaagcataac
     6061 gtcgggtaga tataaattgg cgcgggttgt ttttcgtgac gtacgaattt atctcattca
     6121 gttgctgaca aaaattcgtc acactcttag ccagagacaa tctcttaata cagacaaaga
     6181 gcatctgcgc aaaattgcac gcgcgatgtt ctggctgatg ctgcttatta tttctgcaaa
     6241 aatggcgtat tcactctggc gctatttctc cttttctgcg gaatatacgg cggtttccgc
     6301 atcggcgaat aaaccgctcc gtgcggatgc aaaaccgttc gataaaaatg acgtgcaatt
     6361 agtcagccaa caaaactggt ttggcaaata tcaacccgtc gccgcaccgg taaaacaacc
     6421 tgaatctgcg cctgtggcag aaacgcgtct taatgtggtg ctgcgcggga tcgcctttgg
     6481 tgccagaccc ggcgcggtta ttgaagaagg cggcaaacag caggtctatt tgcagggtga
     6541 acggcttggc tctcacaacg cagtgattga ggaaatcaac cgcgaccatg tgatgctgcg
     6601 ctatcagggg aaaatggaac gtctgagtct ggcagaggag gaacgccccc ctgtggcggt
     6661 gaccagcaaa aaagccgcca gtgacgaagc aaagcaagct gttgctgagc ctgctgccag
     6721 tgcgccagtg gagctcccgg ctgccgtgcg tcaggcactg gcgaaagatc cgcagaaaat
     6781 ttttaactat atccagctta cgcctgtgcg taaggagggg attgtcggtt atgcagtgaa
     6841 accgggggca gatcgttctc tgttcgatgc cagcggtttt aaggaaggcg atatcgccat
     6901 tgcgctaaat cagcaggatt tcactgatcc acgagcaatg attgctctga tgcggcagtt
     6961 accttcaatg gattccattc aacttacggt tttacgcaag ggtgcgcgct acgacatttc
     7021 catcgcactg cgctaaccgc atttaatcca ggagaatcat tcatcgtgtt ttggcgtgat
     7081 atgacgttgt ctatctggcg taagaagaca actggcctca aaacaaaaaa gcgtttactg
     7141 tcgctgatgc tggcagcggc attgtgtagt tcaccggtct gggcggaaga agccactttc
     7201 accgctaatt ttaaagatac cgacctgaaa tcgttcatcg aaaccgtcgg cgctaacctt
     7261 aataaaacca tcattatggg gccgggcgtt caggggaaag tgagtattcg cactatgact
     7321 ccgctcaatg aacgccagta ttaccagtta ttccttaacc tgctggaagc acaggggtat
     7381 gcggtcgtac cgatggaaaa cgacgtgctg aaggtggtga aatcaagcgc cgcgaaagtc
     7441 gagccgctgc cgctggtcgg tgaaggcagc gacaactacg cgggcgatga aatggtcacc
     7501 aaagtcgtgc cggtacgcaa tgtgtcggta cgcgagctgg caccgattct gcgccagatg
     7561 attgacagcg caggctcagg caacgttgtt aattacgatc cctccaacgt gattatgctt
     7621 accggacgcg cctcagttgt ggagcgcctg acggaagtga tccagcgcgt ggatcacgcg
     7681 ggtaatcgca ccgaagaggt gatcccgctg gataacgcct cagcctcgga aattgcccgc
     7741 gtgctggaaa gcctgaccaa aaacagcggc gagaaccagc cagcaacgct gaaatctcaa
     7801 attgtcgccg atgaacgcac caacagcgtg attgtcagtg gtgaccccgc cacgcgagac
     7861 aaaatgcgcc gtctgatccg tcggctggat tcagaaatgg agcgcagcgg caacagccag
     7921 gtgttctatc tcaaatacag caaagccgaa gatctggtcg atgtgctgaa gcaggtcagc
     7981 ggtacgctca cggcggctaa agaagaggcg gaaggcacgg ttggtagcgg gcgtgaggtt
     8041 gtctccatcg ccgccagcaa acacagtaat gccctgattg ttactgcgcc gcaggacatt
     8101 atgcagtcgc tgcaaagcgt gattgaacaa ctggatattc gccgtgctca ggtgcatgtc
     8161 gaggcgttaa tcgtggaagt tgccgaaggc agcaatatca acttcggcgt gcagtggggg
     8221 tcaaaagatg ccggattaat gcagtttgcc aacggtacgc aaatccctat tggtacgctg
     8281 ggggcagcga tttctgcagc caagccgcag aaaggttcca cggtgatcag cgaaaacggt
     8341 gctaccacca taaatccgga tactaacggc gatctctcca cgctcgctca gcttctttct
     8401 ggctttagcg gtacggcggt tggcgtggtg aaaggcgact ggatggcgct ggtacaggcg
     8461 gtcaaaaacg actccagctc gaacgtgctc tccacgccga gtatcaccac gctggacaac
     8521 caggaagcct tcttcatggt gggccaggac gttccggtat taaccggctc taccgttggc
     8581 tccaataaca gcaatccttt caatacggta gagaggaaaa aagtcggcat catgctgaaa
     8641 gtcacgccgc agattaacga aggtaacgcg gtacagatgg tgattgagca ggaagtctcg
     8701 aaagtggaag ggcagaccag cctcgatgtc gtgtttggcg agcgcaaact gaaaaccacc
     8761 gtgctggcta acgatggtga gctgatcgtg cttggcggtc tgatggacga ccaggcggga
     8821 gaaagcgtgg cgaaagtgcc gctgctgggt gatatcccgt tgattggtaa cctgtttaaa
     8881 tcgacggcgg ataaaaaaga aaaacgtaac ctgatggtgt ttatccgccc gaccattttg
     8941 cgtgacggta tggcggcaga cggcgtgtcg cagcgcaaat ataactacat gcgcgctgag
     9001 caaatctacc gcgatgagca aggcttaagc ctgatgccgc acaccgcgca gccgatattg
     9061 ccagcgcaaa atcaggcctt accgccggaa gtacgcgcgt tcctcaatgc cgggagaacg
     9121 cgttaatggt gcctgtagca caggaaacca ccgctaacac cgtgcgtctg ccctacagtt
     9181 tcagccgtcg gtttagcctg gtggcatggt gcgaagcgtc gctggagatc ctccatgttc
     9241 atccgttgtc gctctctgtt ttgcaggagc tacaacgggg gctgaacgcg ccctttacgc
     9301 tgcggcaaat cgacgaggcc gaatttgaac agcggctgaa tgcggtctgg cagcgggact
     9361 cttccgaagc tcgccagctg atggaagatc tcggttccgc cgaggacttt tttaccctcg
     9421 ctgaagaact gccggaaacg gaagatctgc tggaaagtga cgacgatgcg ccgatcatca
     9481 aactgatcaa cgccatgctg gcagaggcaa tcaaagaagg cgcttcggat atccacattg
     9541 agacgtttga aaagagtctg gtgatccgtt ttcgtgttga cggcacatta catgaaatgt
     9601 tgcgccccgg tcgtaaactg gcctcgctgc tggtctcgcg tatcaaggtg atggcgcgac
     9661 tggatatcgc cgaaaagcgc gtaccgcagg atggccgtat tgcgctgctg ctgggcggtc
     9721 gggcgattga cgtccgtgta tctaccatgc cttccgcctg gggggaacgg gtggtgctgc
     9781 gattgctgga caaaaaccag gctcgcctga cgctggagcg tctgggttta agtctcgaac
     9841 tgactgcgca gttgcgccag ctgttacaca aaccgcacgg catttttctg gtgacggggc
     9901 cgaccggttc cggcaaaagc accacgctgt acgctggatt gcaggagctg aacaaccact
     9961 cgcgtaacat tctcacggtt gaagacccta tcgaatacat gattgaaggg atcggtcaga
    10021 cgcaggttaa cacccgcgtc ggcatgacct tcgcccgtgg cctgcgcgcg attttgcgtc
    10081 aggacccgga tgtggtgatg gtcggtgaaa tccgcgatac cgaaaccgca gaaatcgctg
    10141 ttcaggcttc actgaccgga cacctggtac tttccacgct gcataccaac acagcggtgg
    10201 gggcgatcac gcgtttgcag gatatgggcg tggagccttt cctgctctct tccagtctga
    10261 cgggcgtgat ggcgcagcga ctggtccgca cgctgtgctc cgactgccgt caggccgcgc
    10321 ctgccaccga cgaagaaaaa cgtcttatgg ggatctccga tacgcatgcc gtcacgctgt
    10381 accatccgca gggctgcccc gcctgtaatc acaaaggttt tcgcggacgt actgccatcc
    10441 atgaactgat tgtggtggac gccacattgc gtgatttgat ccaccgtcag gccggggaac
    10501 tggagctgga acgttatgtc cggcaacact ctgcgggtat ccgcagtaac ggcattgaga
    10561 aagtgctcgc cggagaaacc tctctcgatg aagttctgcg ggtaaccatg gaggcgtaat
    10621 ggcactgttt tactatcagg cgctggagcg taatggtcgc aaaaccaaag gcatgattga
    10681 ggcggattcc gcgcgtcatg cccgtcaatt gttacgcggt aaagacctca ttcccgtgca
    10741 cattgaagcc cggatgaatg catcggcagg gggattgttg cagcgtcggc ggcacgcaca
    10801 tcgtcgcgtg gcggcggcag atctggcgct gttcactcgt caactggcaa cgctggtgca
    10861 ggcagcaatg ccgctggaaa cctgcttaca ggcggtcagt gagcaaagcg aaaaactgca
    10921 tgtaaaaagc ctcggaatgg cgctgcgcag ccggattcag gaaggttata ccctgtcgga
    10981 cagcctgcgc gaacatcccc gcgtctttga ctccctgttt tgttcgatgg tcgctgccgg
    11041 agaaaaatcc gggcatctcg acgtggtgct caatcgcctt gcggattaca ccgaacagcg
    11101 gcagcgtctg aaatcacgcc tgctgcaggc catgctctac ccgctggtac tgctggtggt
    11161 ggcaacgggc gtggtcacta ttttgctgac ggcagtagtg ccgaaaatta tcgaacagtt
    11221 tgatcatctc ggacacgcgc tacccgcctc cacccgaatg ctcatcgcta tgagcgacgc
    11281 gttacaggcc agcggcgtgt actggctggc gggtttgctg gggcttctgg tgctggggca
    11341 acggctactc aaaaatcctg cgatgcgcct gcgctgggat aaaaccttgc tgcgtttgcc
    11401 cgtgacgggg cgtgttgcgc gcggactgaa tatggcgcgt ttttcccgca cgttaagcat
    11461 cctcaccgcc agcagtgttc cgctgctgga aggcattcag accgccgccg ccgtgtcggc
    11521 aaatcgttat gtcgagcaac aactgctgct ggcggcagat cgcgtccgcg aaggaagcag
    11581 cctgcgcgcc gcgctggcgg atctgcgcct gttcccgccg atgatgctgt acatgatcgc
    11641 ctccggcgaa cagagcggcg agctggaaac catgcttgaa caggccgcgg tcaaccagga
    11701 acgggaattt gatacccagg tggggctggc gttagggctg tttgagccgg cgctggtggt
    11761 gatgatggcg ggcgtggtgc tgtttatcgt catcgccatc ctcgagccga tgctgcaact
    11821 gaacaatatg gttggaatgt aatttacgga gttatcacat gaattcgtta tcccgcacac
    11881 aaaaaccacg ggcaggtttt accctgctgg aagtgatggt ggtgattgtt attcttggcg
    11941 tcctggcaag tctggtggtg cctaacctgt tgggcaacaa agagaaagcc gatcggcaaa
    12001 aagccatcag cgatatcgtg gcgctggaga atgcgctgga tatgtaccga ctggataacg
    12061 ggcgttatcc gaccactgag caggggcttg aggcgctgat ccagcaaccg gccaatatgg
    12121 cggacgcccg caactaccgt accggtggat acatcaaacg actgccaaag gatccgtggg
    12181 gcaatgatta tcagtatctc agcccgggtg aaaaagggct gtttgatgtt tataccctgg
    12241 gggcagatgg tcaggaaaac ggggagggcg ctggcgcaga tatcggtaac tggaatttgc
    12301 aggagtttca gtaatcagtg cctgaacgcg gattcacact tctggaaatc atgctggtga
    12361 ttttccttat cggccttgcc agtgcgggcg tggtacagac gtttgcgacc gcttcagagc
    12421 cgcctgcgaa aaaagcggcg caggcttttc tgactcgctt tgcgcagttt aaggacaggg
    12481 cagtgatcga agggcaaaca ctcggtgtgc taatcgaccc gccaggttac cagtttatgc
    12541 agcgtcgtca agggcaatgg ttgcccgttt ctgcgacccg gttatcggca caggttacgg
    12601 tgccgaaaca ggtgcagatg ctgttacaac ccggcagtga tatctggcag aaggagtatg
    12661 cgctggagat gcaacgtcgt cgcctgacgc tgcatgatat tgaactggag ctgcaaaaag
    12721 aggcgaaaaa gaagacaccg cagatccgtt tttcgccttt tgaacccgcc acgccgttta
    12781 cgctgcgatt ttattcggcg gcgcaaaacg catgttgggc ggtaaaactg gcgcacgatg
    12841 gcgcgttatc tctcaatcaa tgtgatgaga ggatgccatg aagcgtggat ttaccttgct
    12901 ggaagtaatg ctcgcgctgg cgatttttgc gttggccgcc atggcggtgt tacagatcgc
    12961 cagcggcgcg ctgagtaatc agcacgttct tgaggaaaaa acggtagcgg gttgggtagc
    13021 tgaaaatcag accgcgcagc tttacctgat gacccgtgaa caacgagcgg tcaggcacca
    13081 gggcgagagt gatatggcag gaagccgctg gtactggcga accacaccgc tgaataccgg
    13141 taacgtgctg ctccaggcgg tggatattga agtcagtctt catgacgact tttcgccagt
    13201 gattcagtca cgacgcgcct ggtttagcgc cgtgggaggc caacagtgag aagggctcgc
    13261 gctggtttca cgttactgga aatgctggtg gcgatagcca tttttgcctc actggcactg
    13321 atggcgcagc aggtgacaaa cggcgttaca cgcgtgaata gcgccgtcgc cggacacgat
    13381 caaaaactaa acctcatgca gcaaacgatg agttttttga accacgatct gacccaaatg
    13441 atgccgcgtc cggtaagagg cgatcagggc cagcgtgaac cagcgttact ggcgggcgct
    13501 ggcgtgctgg cgtctgagag tgaagggatg cgttttgtac gtggcggcgt ggttaatccg
    13561 ttgatgcgtc tgccgcgcag taatctgctc accgtcggtt accgcattca tggtggttat
    13621 ctcgaacggt tagcctggcc actgaccgat gcggcaggca gcgtgaagcc aacaacgcaa
    13681 aaattaatcc cggcggattc gctccgtttg cagttctacg acggcacgcg ctggcaggag
    13741 agctggtcat cagtgcaggc gattcctgtg gcagtacgca taaccctgca ttcgccacaa
    13801 tggggcgaaa ttgaacgcat ctggttgtta cgcgggccgc aattatcatg atcaccttac
    13861 caccaaaacg cggaatggca ctggtcgtgg tgctggtatt gctggcggtt atgatgctgg
    13921 tgaccatcac gctttccggg cggatgcagc aacaacttgg gcgaacgcgc agccagcagg
    13981 agtaccagca ggcgctgtgg tacagcgcca gtgcagaaag cctggcgctg agcgcgctca
    14041 gtctgagcct gaaaaatgaa aagcgtgtgc atctggcaca accgtgggct tctggcccgc
    14101 gttttttccc actgccgcag gggcaaatcg ccgtcactct gcgtgacgca caggcctgct
    14161 ttaacctgaa tgccctcgct cagccgacga cggcgtcgcg tccgctcgcg gtacaacaac
    14221 tgattgccct gatctcgcgc cttgatgtac ctgcttatcg ggccgaactg atagccgaaa
    14281 gcctgtggga gtttattgac gaagaccgca gcgtgcagac gcgtctgggg cgtgaagaca
    14341 gcgagtatct cgcccgctcg gtgccgttct acgccgctaa tcaaccgctg gcggatatca
    14401 gcgagatgcg cgtggtgcag ggaatggacg ccgggcttta tcaaaaactg aaaccgttgg
    14461 tctgtgcgct gccgatggcc cgccagcaaa tcaacatcaa tacattagat gtcacgcaaa
    14521 gtgtgattct tgaggcgctg tttgacccgt ggttaagccc tgttcaggcg cgggcattat
    14581 tacaacaacg tccggcgaag ggctgggaag atgtcgatca gtttcttgct cagccgctac
    14641 ttgcagacgt cgatgagcgt actaaaaaac agctaaaaac cgtcctgagc gtggacagca
    14701 attacttctg gctgcgttca gatatcaccg tgaatgagat tgaactgacg atgaattcgt
    14761 taattgtccg catgggccca caacactttt ctgttctctg gcatcagaca ggagaaagtg
    14821 agtgagttcc atgcttgaga tttttttccc gctttgcgcc gctgatccca tccgttggca
    14881 gcgccataca cccgacgtgg agcacggtat ctggcctgac gttgctgacg aacgtctcca
    14941 gcaatggctg caaactgatg cgattcgact ctacattccc ggcgaatgga tcagcgtctg
    15001 gcaggttgaa ctgcctgatg tgccccgcaa gcagataccg acgattctgc ccgccttgct
    15061 ggaagaagag ctgaaccagg atatcgatga actgcatttc gccccgttga aaatcgacca
    15121 gcaactggca accgtagctg tgattcacca gcagcatatg cgcaacattg cgcagtggtt
    15181 gcaggcaaac ggcatcaccc gcgctaccgt cgcgccggac tggatgtcta ttccttgtgg
    15241 gtttatggct ggcgatgcgc aacgggttat ctgccgcatc gatgaatgcc ggggatggag
    15301 cgccgggcgg gcgctggctc cggtcatgtt ccgcgcacag ctcaatgagc aggatatacc
    15361 gctttcacta accgtggtcg gcattgcacc ggaagaactg tctgcatggg ctggtgcgga
    15421 cgccgaacgc ctgaccgtta cggctctgcc agccattatc acttatggcg aaccggaagg
    15481 gaacctgcta acagggccgt ggcagcctcg tgtcagctac cgaaaacagt gggcgcgctg
    15541 gcgggtgatg attctgccga tattgctgat tctggttgcg ctggtagtgg aacggggcgt
    15601 gacgttatgg agcgtcagcg aacaggtggc gcaaagccgc acccaggcgg agaaacagtt
    15661 cttaacgcta ttcccggagc agaagcggat tgtgaattta cgctcgcagg tgacgatggc
    15721 gctgaaaaaa tatcgcccac aggctgatga taccgatctg cttgccgagt tgtctgcgat
    15781 cgccagtacc ctgaaatccg catcactttc cgacattgag atgcgtggtt tcacctttga
    15841 tcaaaaacgc cagacgcttc acctccaact gcgggctgcg aactttgcca gcttcgacaa
    15901 actgcgtagc gcactggcgg cagattatgt tgtgcaacag gacgcgttac agaaagaggg
    15961 tgatgcggtt tccggcggcg taacgttgcg gaggaaataa catgttacgc gataaattta
    16021 ttctctattt tcagcaatgg cgtgaacgcc agttaagccg tggcgaacac tggctgacac
    16081 aacacctggc ggggcgttct ccgcgtgaaa aaggcatgtt actggcagcg gtagtgttcc
    16141 tgtttagcgc cggatattac gttctcatct ggcagccgtt gagcgaacgg attgagcaac
    16201 aggagacgat gttgcagcag ctggtggcga tgaacgcgcg actgaagagc gccgcgccgg
    16261 atattattgc ggcgcgaaaa tccggcacga caacgccagc acaggtatcg cgggtcatca
    16321 gcgacagcgc ttccgcgcat tctgtggcca ttaagcggat agccgagcgt ggggagaata
    16381 ttcaggtctg gatagatccg gtggtgttta atgacctgct gaactggtta aaagcactgg
    16441 atgaaaaata tgcgctgcga gtgacacaaa ttgatgtcag tgctgctgag aagcctggga
    16501 tggtgaatgt gcagcgactg gaatttgggc gggggtaaag tatcaggaga ttaatatcaa
    16561 taaaattact ctcgttaacg tggcgagagt aattccccca gactttataa gctaattttt
    16621 ttcgtgaagg ttattgtcaa atccaaaagc aaaaacccgc cttgtgggcg ggttctttag
    16681 aatagtggtg cccggactcg gaatcgaacc aaggacacgg ggattttcaa tcccctgctc
    16741 taccgactga gctatccggg caacggggcg cattaaacct gattcgcctc gtctcgtcaa
    16801 tcaaatttct tcaatttagt gcagactgca caatctatca tcactttgcc gctattgcac
    16861 gcattgtcag gcaaaccagg cggtccaggc ggcggaaagc ggcattgcca gaaggagtgc
    16921 tggaagcatg ttgaccaccg ggaacatttt aatgccacag atgcgtaatc cggttgccag
    16981 cagcagtaaa ccgcctacgg cgctgaagtc tgccatcatc gacggtgtgg tcagcggtaa
    17041 tatcagcgcg gcagcccacg ccagtgttaa ctggatgatc agtaatggaa tgctaatcac
    17101 cgacaccgca atacccagcg agcaggcgaa gatcatcgcc gtaaagaaat caagaaatga
    17161 cttggcgatt aaaatactcg gatcgccggt cattccttcg ttcatcgccc cgaagattcc
    17221 ggtgccgctg gcgcaaaaca gaacaataat cgcgacaaaa ttctgaataa aagattcatg
    17281 cgctggcttc ttacgcgagt ggtgaaacag attatgcgct ttggcgacca ctgtattgac
    17341 gcctttttcc agcagacaaa tttcgccgat taatgctccg agtaaggttg ctaaaaccat
    17401 cgccggaagg ttggcgcatt tcaccaccag taaaatacca atccccagcg atgccagacc
    17461 aaaaattgat gtcatggaga cgcggatacg ttccggtaag cgttggctaa ggagtgcgcc
    17521 gaaaacgcca cccagtaaga ctgcacttgc gttgataaaa gggccgatga ccacaagagt
    17581 tcctgttagc tgtattgttt gattttctta ttatgaactt ttggcgccgt cgatggcttg
    17641 tttgttcgca aagtcctggc ttgcacgctt tagcgaaagg tgccatgatt gcgcgaattt
    17701 tctcctctct gtacggagtt tgcccgatgc acgccacctc cttacattct ctcgcttatc
    17761 gccgtttcgc gcgaaactct tctcttttta tgctactgct catgcggtga agttaacgca
    17821 cgctcactgc aggacaacag taaaatcaga gcgtttctgc ttttactgat gtctggcggt
    17881 cggagctggt gaccagtttg acccatatct catggggcag ggttttccac cttgtccggt
    17941 attcttactt cccctaaacg ggttttgcgc ttatgaaatc aatgaatatt gccgccagta
    18001 gtgaactggt atcccgactt tctactcatc gctgcgtggt agcgttggga gatactgatt
    18061 ttacggacgt cgcggcagtc gtcattaccg ctgcggatag tcgcagtggc attcttgcgt
    18121 tgcttaagcg caccggtttt catctaccgg tgtttttgta ttccgaacat gctgttgaat
    18181 tacctgcggg cgttacggcg gtaatcaacg gcaacgagca gcagtggctg gagctggaat
    18241 ccgcagcctg tcagtatgaa gagaatttgc tgccaccgtt ttatgacacg ctgacgcagt
    18301 acgttgagat gggtaatagc acctttgctt gccctggaca tcaacatggt gcgttcttta
    18361 aaaagcatcc tgccggacgc catttttacg atttctttgg cgagaacatc tttcgcgccg
    18421 atatgtgtaa cgctgacgta aaattgggcg atctgcttat tcatgaagga tcggcgaaag
    18481 atgcgcagaa atttgcagcc aaagtctttc atgccgataa aacctatttt gtgctgaacg
    18541 gcacatcggc agcgaataaa gtggtgacga atgcgctgtt aacgcgtggc gatctggtgc
    18601 tcttcgaccg taacaaccat aagtcgaatc atcatggcgc gttgattcag gcgggggcga
    18661 cgccggtcta tctggaagct tcacgtaacc cgtttggttt cattggcggt attgatgcgc
    18721 actgttttaa tgaagagtat ctgcgccagc aaattcgcga cgttgcgcca gaaaaagccg
    18781 acctgccgcg cccgtttcgc ctggcgatta ttcagctggg aacctatgac ggcactgtct
    18841 ataacgcccg tcaggtgatc gataccgttg ggcatctgtg tgattacatt ctgtttgatt
    18901 ccgcgtgggt cggttacgaa cagtttatcc cgatgatggc ggatagctcg ccgctgctgt
    18961 tagaacttaa cgaaaacgat ccggggatct ttgtgactca gtcggtgcac aaacagcagg
    19021 cgggattctc acagacgtcg cagatccata aaaaagataa ccatatccgt ggacaggcgc
    19081 gtttttgccc gcataagcgg ttgaataatg cctttatgct ccatgcttct accagcccgt
    19141 tctatccgct gttcgccgcg atggacgtta acgccaaaat tcatgaaggg gagagtgggc
    19201 gtcggctgtg ggctgagtgc gttgagttgg gtattgagtc gcgcaaggct attcttgcgc
    19261 gctgtaagct gttccgcccg tttatcccgc ccgttgttga tggcaaattg tggcaggatt
    19321 atccgacgtc agtgttagcc agcgaccgcc gttttttcag ttttgagccg ggggcgaagt
    19381 ggcacggctt tgaaggatat gccgcggatc agtattttgt tgatccgtgc aagctgttac
    19441 tcaccacgcc gggtatcgat gccgaaaccg gcgaatatag cgattttggc gttccagcga
    19501 cgattctggc gcactatctg cgtgagaacg gcattgtgcc ggagaagtgc gatctcaact
    19561 ccattctgtt cttattaact ccggcggaaa gccacgagaa gctggcacaa ctggtggcga
    19621 tgctggcgca gtttgaacag catattgagg atgactcgcc gctggctgag gtgttgccga
    19681 gcgtttataa caaatatccg gtgcgctatc gcgactacac cctgcgccag ttgtgtcagg
    19741 agatgcacga tctgtatgtc agtttcgacg tcaaagacct acaaaaagcg atgttccgcc
    19801 agcagagttt cccgtcagtg gtgatgaatc cccaggatgc gcatagcgct tatattcgcg
    19861 gtgaagtgga gttggtgcgg attcgtgatg ccgaagggcg aattgcggca gaaggggcgt
    19921 tgccttatcc acctggcgtg ctttgcgtgg tacccgggga agtctggggt ggggcggttc
    19981 aacgttattt ccttgcgctg gaagaaggcg tgaatttgtt gccgggattt tcgccggagt
    20041 tgcaaggtgt ctatagcgaa accgatgcgg atggcatgaa acggttgtac ggttatgtgt
    20101 tgaagtaaga ataaaaaaac gggtcacctt ctggcgaccc gtttttcttt gcgtaattag
    20161 tggctaaccg tctgtgtacc tgtcgggaca cgaacgtgtt tatatttgaa catcgccatg
    20221 aacgcgaagg ccagaaccac ggagtaacct gcgaaaatca gccatacggt ctgccagtcg
    20281 gtaatgccgt tttgggtgta catctcaacc actttaccgc tcacgatgcc gccgaggata
    20341 cagccgaagc cgttggtcat catcaggaac atcccctgcg cgctggcgcg aattgccggg
    20401 ctaacttctt tttcgacaaa caccgaacca gagatgttga agaagtcgaa tgcgcaaccg
    20461 taaacgatca tcgacagtac cagcagtaca gtaccgaacg gagtcgggtc gccgtaagca
    20521 aacagagcaa aacgcaggat ccacgccaca atactgatca tcattacgtt cttaataccg
    20581 tagcggctta agaagaacgg gatggtcaga atgaacaggg tttcagagat ctgcgaaatc
    20641 gacatgatga ttgacgcatg ctgcacgatg aagctgctgg caaacatcgg atctttgtcg
    20701 aagctgtgca ggaaggtatt accgaacatg ttggtaatct gcagttccgc gcccagcagc
    20761 attgagaaga tgaagaagat tgccatacgc ttgtttttaa acagcgcgaa tgcatcgagg
    20821 cccagcaggg ttgtccagct ctgattcgct tgctgtttag caaccggaat atgcggcaga
    20881 gtcagggtaa acagaaccag aatggcggaa agcgctgcgc caatatacag ctgcatgtgg
    20941 cttaattcga agccggacag gctcaccacc cacattgcca tgataaagcc gatggtgccc
    21001 cagatacgga ttggcgggaa gtcagtaacg atatccatcc cggcattttg caggcgatag
    21061 tatgagatgg tattgattaa cccaagcgtt ggcatataag caaacgagtt aatcaatatc
    21121 acaaggaaca tcgcctccgg cgtagtgacc tctgccgcca tgaacagtgt gatagcgcca
    21181 atggtgtggc aaatggcata gacccatttc gcacttaacc atttgtcggc cacaatcccc
    21241 agcagcgcag gcataaagac cgcggcgata cccagtgagc tataaactgc gccgatagaa
    21301 gcaccgtcaa acttcagggt aacaaacata taggagccga gggtcgtcag ccaacttccc
    21361 cacagacaga actgcagaaa agagaggatt ttcagctgca gcttaagatt catgttaatt
    21421 tcctcacatc gtgatgcgga tgagtgtttg aaaaagcaca tttgaccgtg cgagatgatg
    21481 cgattctatc aacttcgcgg actggttttt tgttacctgt ggcaaataat tgcaaacatt
    21541 cctaatttgc aaaacgaaat tgtgatgtgg ataacatttt tacccctgag catcgtcagg
    21601 ggcggttagt ggaattatcg gcggcggtaa gatttttgcg cggtattcac tttataaaga
    21661 taacggcgag attccgcaga gggatggcgg gtcgtcagcg tctgataaac atcgcccggc
    21721 gtcatggtgt taataatatt ggcagcctga atcttatcat tcgaaaagac tcgtagcacg
    21781 ctgcctgcgc cgccgttata ggcggtgatg acggcataac gccgcgatgt tgggttatca
    21841 attccgccga gataaacgtt gttcagcatc gccagatacg cggtgccagt atcaatattg
    21901 ctggcaggat caaacaagaa actgcggctc ggcgtgccgg atttcccctg cgagcggaac
    21961 acatctttcc cggcggtatg ttgtaccacc tgcattaatc ccagcgcatc ggaacggctg
    22021 accgcatacg ggttaaagga agattcagtc tgcataattg ccagaatcag cgactcatca
    22081 acgccatatt tgcgtgacgc ctggcggacc ataccgagat atttgtgcgc acgtttatca
    22141 aggtggttcg gcaccatgtt aatggtgacg ctgtagatga tacgcagccc gttgctgcgg
    22201 ctcttcagac ggtttttcag cagataatcc gcgaagttgc tcgcgcgacc ttcccagcga
    22261 atcggctgcc cggtgttgtc caccacctga ccgtaaagga aaggttcttt cgaaatcgta
    22321 atatcatcaa cgtcggaata gagatcgacc gaactcggat cgtcacccat cagcaacgtt
    22381 ttgattattg cccggcgcaa atgcgcggca ggttctgtcc cggcgatggt ttcgatagta
    22441 atcgtaccgt catcgaagtt gatgtggctg cgggtctgat attgatcggt gtatttcacg
    22501 tagtccttag gaccggcgat caccacctct ttgaagcccc agatgttctc aatattgtgg
    22561 gcaaattgcc ccatcagaat atcaaaaccg ttggtatctt tgacccaggc ttcgttatag
    22621 gtatcgtctt ttttggtcgt cgaacaggag atgagcaacg gcgcaatcaa agccagcgcg
    22681 agatattttt tcatcattcc gggtgcgtgt tgtgtttgtt ttatgcaagt tgccggaggc
    22741 gtgctccggc actgttttta ttttttatct tccggcgtat agccctcgat atgcacttct
    22801 ttaccctcga acaggaagtt gaccatctcc tgctccagca gcttgcggtg ctcggcattc
    22861 atcatgttga gtttcttttc attaatcagc atggtttgtt tgtgctgcca ctgcgcccag
    22921 gcttctttgg agatctcgtt atagatgcgt tttcccagtt cgccggggta cagctgaaaa
    22981 tcctgacctt ctgcttcacg ttgcaggaaa gtacaaaaaa tcgttctgct cataaatcat
    23041 cctctttatc gactcacgcg ctaaaccggc gcgccagtgc gtaactgctg taacaaacgc
    23101 tccacgggag ccgccaggcc aactgacggc ggttgcgcta agttatacca gagcgcattg
    23161 tcttcatcca tgcagccgat gaatgacgac acgggaagcc acataggcac aatatctaag
    23221 tggaaatggc tgaaggtatg ccgaaacgcg gtcagctgcg tcaggttatc ggcagcaatc
    23281 tgtcgttgcg ccagccactg ccgcaaactt tcttcatcgg caaactgcgg gaaacagtat
    23341 aaaccgcccc acaatccact cggcggacgc tgcgccagca atacttcatc ttcgtgctgt
    23401 aacagcaaaa agtagccggt gcgctctggc agcgtctgtt tcggtttttt gcccggataa
    23461 agcgaccagc tattgttggc ggcggcaata catccgtttt gtagcggaca gagcgagcat
    23521 ttcggcttag agcgcgtaca aatcatcgca cccaaatcca tcatcgcctg gttaaaccgt
    23581 tccacgccaa ccgcgggcgt cacctgctcg ctcaaactcc atagtttatt ctcgacctct
    23641 tttttcccgg gccagccgct tacagcatag cagcgcgcca gcacgcgttt gacgttaccg
    23701 tcgagaatcg gaaagtgctt acccagagaa agcgagagaa tcgcgcctgc ggtggaacgc
    23761 ccgacgcccg gcagtgctgc aacttcctca aaggtttccg ggaatttacc gccgtgtaag
    23821 gccgccactt gttgtgcagc tttatgcaga ttgcgcgcgc gggcgtaata gccaagcccg
    23881 gtccacaagt ggagaacttc gtcgagcggc gcattggcga gatcggtcac cgttgggaag
    23941 cgtgccataa agcgttcaaa ataggggata acggtcgcaa cctgagtttg ttgcaacatc
    24001 acttctgaga gccatacttt gtagggcgtc ttgtcaattt gccagggcag cgttttccgc
    24061 ccgtatttat cgtaccagtc cagaacctgg gctgaaaatt gcgacgcttg catggtcacc
    24121 gaattcactg ttgttggggg caagattgca gcacagcgac ggcagggtgt aaaccggaac
    24181 tttccgcagc cacggcctta tcatgcttgc atccggcaac taactttgga taatgcccgt
    24241 tttcagaaca ctttcacaag cgactaaatc tttatgaaaa acgacgtcat ctcaccggaa
    24301 tttgatgaaa acggccgccc gctgcgccgt atccgtagtt ttgtgcgccg ccaggggcga
    24361 ctgaccaaag gccaggaaca tgcgctggaa aactactggc cggtgatggg cgttgagttc
    24421 agcgaagata tgctggattt ccctgtgctt tttggccgtg aagcgccggt gacgcttgag
    24481 attggttttg gcatgggcgc gtcgctggtg gcaatggcta aagatcgccc cgagcaggac
    24541 ttcctcggca ttgaagtgca ttcaccgggc gttggtgcgt gcctgtcttc tgcgcatgaa
    24601 gagggcttaa gcaacctgcg cgtgatgtgt cacgatgcgg ttgaagtgct gcataaaatg
    24661 attcctgaca attcattgcg catggtgcag ctctttttcc ctgacccgtg gcacaaagcg
    24721 cgccataata aacgccgtat cgttcaggtg ccgtttgccg agctggtaaa aagcaaactg
    24781 cagctggggg gcatattcca tatggcgacc gactgggaac cttatgcgga acatatgctt
    24841 gaagtgatgt cttctattga cggttataaa aacctgtcag agagcaatga ttacgtaccg
    24901 cgtccggcat cacgtccggt gacgaaattt gaacaacgtg gtcatcgtct tggtcacgga
    24961 gtatgggact taatgttcga gagggtgaaa taatggcaaa gaaccgtagc cgtcgtctgc
    25021 gtaaaaaaat gcacatcgac gaattccagg aattaggatt ttcggtggca tggcgattcc
    25081 cggaaggtac atcggaagaa cagattgata aaaccgttga tgactttatt aacgaggtta
    25141 tcgaaccgaa caaactggcc tttgacggca gcggttatct ggcctgggaa ggtctgatct
    25201 gcatgcagga aatcggcaaa tgcaccgaag aacatcaggc gattgtgcgt aagtggctgg
    25261 aagaacgcaa actggaagag gtacgcacca gcgaactttt cgacgtttgg tgggactaag
    25321 aaagcatacg ggcgatgaca aatgcaaaac tgcctgatgt gctacgctta tcaggcctat
    25381 aaagatgcac gatcgagtag gctggataag gcgtttacgc cgcatccggc atggaaaacg
    25441 cgcactttgt tatcaatctg gggccagcaa atgctggcct gatttgttct tgagggaaga
    25501 ctatgatgcg caaaatgctg ctggcggcag cactttcagt gacggcaatg accgctcacg
    25561 ccgactacca gtgcagcgtc acgccgcgtg acgatgtgat tgtcagcccg caaaccgtgc
    25621 aggtgaaggg cgaaaacggc aatctggtga tcacgccaga cggcaacgtg atgtataacg
    25681 gtaagcaata ttccctgaat gccgcccagc gcgagcaggc gaaggattat caggctgaac
    25741 tacgtagcac cctgccgtgg attgatgaag gcgcgaaaag ccgcgtcgag aaagcccgta
    25801 ttgcgctgga taaaattatc gttcaggaga tgggcgaaag cagcaaaatg cgcagccgtc
    25861 tgaccaaact tgatgcgcag ctgaaagagc agatgaaccg cattatcgaa acgcgcagcg
    25921 atggcctgac gtttcactat aaagccattg atcaggttcg cgccgaaggc cagcaattag
    25981 tgaatcaggc aatgggcgga attttacagg acagcattaa tgaaatgggc gcgaaagcgg
    26041 tgctgaaaag cggcggtaac ccattacaga acgtgctggg aagcctgggc ggtctgcaat
    26101 cctcaatcca aaccgagtgg aaaaagcagg aaaaagattt ccagcagttt ggcaaagatg
    26161 tttgtagccg cgttgtgact ctggaagata gccgcaaagc cctggtcggg aatttaaaat
    26221 aatcctctat tttaagacgg cataatactt ttttatgccg tttaattctt cgttttgtta
    26281 cctgcctcta actttgtaga tctccaaaat atattcacgt tgtaaattgt ttaacgtcaa
    26341 atttcccata caaagctaag ggataatgcg tagcgttcac gtaactggag gaatgaaatg
    26401 gagtttttca aaaagacggc acttgccgca ctggttatgg gttttagtgg tgcagcattg
    26461 gcattaccca atatcaccat tttagcaacc ggcgggacca ttgccggtgg tggtgactcc
    26521 gcaaccaaat ctaactacac agcgggtaaa gttggcgtag aaaatctggt taatgcggtg
    26581 ccgcaactga aggacattgc gaacgttaaa ggcgagcagg tagtgaatat tggctcccag
    26641 gacatgaacg atgatgtctg gctgacactg gcgaaaaaaa ttaacaccga ctgcgataaa
    26701 actgacggct tcgtcattac ccacggtacc gacacgatgg aagaaaccgc ttacttcctc
    26761 gacctgacgg tgaaatgcga caaaccggtg gtgatggtcg gtgcaatgcg cccgtccacg
    26821 tctatgagcg cagacggtcc attcaacctg tataacgcgg tagtgaccgc agctgataaa
    26881 gcctccgcta atcgtggcgt actggtagtg atgaacgaca ccgtgcttga tggccgtgat
    26941 gtcaccaaaa ccaacaccac cgatgtagcg accttcaagt ctgttaacta cggtcctctg
    27001 ggttacattc acaacggtaa gattgactac caacgtaccc cggcacgtaa gcacaccagc
    27061 gacacgccgt tcgatgtctc taagctgaat gaactgccga aagtcggcat tgtttataac
    27121 tacgctaacg catccgatct tccggctaaa gcactggtag atgcgggcta tgatggcatc
    27181 gttagcgctg gcgtgggtaa cggcaacctg tataaaaccg tatttgacac ccttgcaacc
    27241 gctgcgaaaa acggcactgc agtagtgcgt tcttcccgcg taccgacggg cgctaccact
    27301 caggatgccg aagtggatga tgcgaaatac ggcttcgtcg cctctggcac gttgaacccg
    27361 caaaaagcgc gcgttctgct gcaactggct ctgacgcaaa ctaaagatcc gcagcagatc
    27421 cagcagatct tcaatcagta ctaatcactt cgccccggta tcgtgccggg gcttattcac
    27481 ttcagactca cgtccattgc caattttcat taccctaatg ataatcaccg gaataaatta
    27541 ttccgcgcga gggttttcgg gtgaaaaagc aatggattgt tggtacggcg ctgtttatgt
    27601 tgatgactgg taatgtccgg gcagatggtg aaccgccaac tgaaaatatc ttaaaagatc
    27661 aattcaaaaa gcagtatcac ggcattctca agcttgatgt catcacctta aaaaatcttg
    27721 atgctaaggg taatcaggcc acctggtcag cggaaggcga tgtctcttcc agtgacgatc
    27781 tctatacctg ggtcggtcag ttggcagatt acgagctgct cgaacagacc tggacgaaag
    27841 ataaaccggt gaaattctcg gcgatgttaa ccagtaaagg aacgcccgcg tctggctggt
    27901 cggtgaactt ttactctttt caggcggcag ccagcgatcg tgggcgggtg gttgacgata
    27961 tcaaaaccaa taataaatat ctgatcgtga atagcgaaga tttcaattat cgctttagtc
    28021 agcttgagtc tgcattgaat aaccagaata actcgattcc tgcactgaaa aaggatgtaa
    28081 aagcgcttga taagcaaatg gtggcggcgc agaaagcagc ggatgcatat tggggaaaag
    28141 atgcaaacgg taagcaaatg acccgcgaag acgcctttaa aaaaattcac caacagcgtg
    28201 atgattttaa taaacagaac gatagcgagg cgttcgcggt taaatatgac aaagaggttt
    28261 atcaaccggc gattgcggca tgtcataaac agagtgaaga gtgttatgaa gtgccgattc
    28321 agcagaagcg agatttcgat atcaacgaac aacggcgaca gacttttctg caatcataaa
    28381 aacttagccg taaattgcag gatgactgga taacgctcga aaaagggcaa tatccgttaa
    28441 cgatgaaagt ttcagaaata aacagtaaaa aagtcacgat tttgatgaaa attgacgata
    28501 tcaaccaggc taatgaacgc tggaaaaaag ataccgaaca attgcggcgc aatggggtga
    28561 ttaagtaact catgttaagt acctttgccg gataatataa aaagttatcc ggcaaattat
    28621 tttttacatt agccctagta gctgtggaat gaacaaggat atttgcggaa tgtaagtgat
    28681 catcatcaac gctactagca atgcagcata aaatggcagc aatggtttga ttaaatgttg
    28741 aatcttcaca cccgagatag aacaaccaac aaatagcgca ctacccaccg gcggtgttaa
    28801 cagtccaata caaaggttgg ccaccatcat aataccgaaa tgtacagggt ccattcccag
    28861 ctcttgtgca atcggcaaga agatcggtgt aaagattagt acagccggtg tcatgtccat
    28921 aaagatgccg acaattaata agacgatatt aatcagcagc aatatgatca gtgggttatc
    28981 agaaatcccc atcaacgcgt cgctgatcat ataaggaata tcagcattgg tcattgccca
    29041 ggacatgccg acggaaaaac cgatcaacag taatacaatt gaagtggtaa caaccgattc
    29101 cagaatcagt ttgggtaagt cgcgccattt aacctcccgg taaatcagca ccgagagcat
    29161 aaatgtgtag acaacggcaa tcgctgacgc ttcagttgcg gtaaaaacac cgcccagaat
    29221 gccacccatc acaatgaaca ccagaagcaa acttggtaag gcatccagga acgctttaca
    29281 tgcctgagca aaggttgctc tttctgaaag tgggtaacca cggcgtttag caataatggc
    29341 gcagacaacc attaccgcga gacccattaa gatccctggc aaatatcccg ccataaatag
    29401 tgatgctaca gaaacaccac cagccgtcag ggaaaacacg attaacacgt ttgagggcgg
    29461 tatcagtaat ccagtaatac aggatgagac gtttacagcg gtagaaaatg tcggatcata
    29521 accttctttg gtctgaatgg gattaagtgt gccaccaacg gcagcagccg ccgcaacggc
    29581 tgaacctgaa atagaaccaa acatcatatt cgccaggacg ttcacatgac caagtgaacc
    29641 aggaacccgg ccaaccataa ccttggcgag gttaatcaaa cgtattgcaa taccgccgct
    29701 gttcatcagc gttccggcaa agatgaaaaa cgggattgcc agcagggcaa agttatccag
    29761 tccatttgcc agtcgttgcg aaacagtgat tgccgcgata tcccagggaa acatcaaaag
    29821 cattgaagct acagtcgcaa taccgattga gaacgaaatg ggcacaccaa gaaagaccag
    29881 cacaaaaaaa cagccaaaca gtgtaagcgc gatgtaacta tccattaact ggcctcctgc
    29941 gcaggttgtt tcagttgttg aatgctttga ttaataaaac acaaagcata aaacatcatt
    30001 accactccac ttaatggcag aatgatatat acgtatccca tcggtatttg catcgcggca
    30061 gatacttgtg atgttgccag cgttttatca attaatttca ggccaccagt cacaatgacc
    30121 gaaccggcaa agcctaaaat gagaaagtta atgactatac taagaagtaa ttgtttgcgc
    30181 ttattcaggg cgagagcaaa taaatcaata gagagatgtc gctgcgcgcc gacggtatat
    30241 gcagcaccta ataatcctac ccaaatcatc aaaaagcgtg ccagctcatc ggttaatgta
    30301 cttggatggt taagtatgta gcggctaaat acttgccaga ctacgcatac aaccagagca
    30361 agcattacgg cgacagaaaa cgcggcaatg acgcggtcta ccgcaagttt caggcggtta
    30421 aatatcattg taagcatcct ggaaaatcag ggcctgagac tggcccggaa tattattgag
    30481 cagcgttgtc gaatttttct aacaatgcag cttgttttgg atctttttta aagtcgtcaa
    30541 aaaggggttg tacggcttta cggaatggtg ctttatccac ttttacaatt tcacccccca
    30601 tagctttcgc ctgagcacga gtgtcagcgt caattttttc ccacaatttt tgttgataag
    30661 cttcggattt cttcgccgct gtttcgataa tttgttgctg ttccggggtt aatttattcc
    30721 atgttttggt ggagataacc aaaaagtcag gaattgaggc gtgttcatct tcagaaaaga
    30781 ctttggcaat ttcgatatgt cgagtctgca cctaggaagg tacattattt tctgctccat
    30841 ccactacgcc ttgttgcatc gcagtataaa cttcgccaaa tgaaatcggg gttggtgacc
    30901 cacccattag ttcaatcatt ttgatggtgg tcggacttgg ttgaacacga attttcagcc
    30961 ctttaaggtc ttctggtttg gtaatcggtt ttttcgcata gaaactgcgt gtaccagcga
    31021 cataagccga taaagcaaaa aagcctttat cttttgttga atccataatt tctttaccaa
    31081 cttcaccaaa aacaactttg ttgaagtgag tctgatcttt aaataagaac ggcaaattat
    31141 agatggcata aatattatca aaagactcca gatcgctggc ggaacctttg gtcatatcca
    31201 gtgcgccatt ttgcaacaac tccatggttt ctcgcgcatt acccatctga ctgctgggat
    31261 aaatgcggat caccatatta ccttttgaca gttgttttac ctctttcgcc agttcttcaa
    31321 aactctgatg cacaacatga cttctttcca ggttgtgtgc taactttaac gtcacttttt
    31381 ctgcggcgtt tacgccagtg ctaaccaaag ccagcaagga tgcgcagact aatgagcggg
    31441 taaatgacct catcttcata aaaaactccc tgttgaagga tggaaattgg ttgatatcta
    31501 attgtgctta ttagtgcctt gtacgacaaa tttgctaacc tgtctgacaa cttttctata
    31561 tttatcacaa tt
//
LOCUS       DADSYM010000042        30845 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2415
            SAMN08039579-rid11723173.denovo.042, whole genome shotgun sequence.
ACCESSION   DADSYM010000042 DADSYM010000000
VERSION     DADSYM010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039579
            Sequence Read Archive: SRR6362533
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 30845)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30845)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980162.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 03:39:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,484
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 4,347
            CDSs (with protein)               :: 4,347
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 43 of 85
            Pseudo Genes (incomplete)         :: 37 of 85
            Pseudo Genes (internal stop)      :: 19 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30845
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039579-rid11723173.denovo.042"
                     /strain="LGE2415"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8302 N 95.9628 W"
                     /collection_date="2015-08-24"
     gene            complement(79..1195)
                     /locus_tag="J1Y83_001615"
                     /pseudo
     CDS             complement(79..1195)
                     /locus_tag="J1Y83_001615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016237780.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
     gene            complement(1435..2304)
                     /gene="sucD"
                     /locus_tag="J1Y83_001616"
     CDS             complement(1435..2304)
                     /gene="sucD"
                     /locus_tag="J1Y83_001616"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HBA7629768.1"
                     /translation="MSILIDKNTKVICQGFTGSQGTFHSEQAIAYGTKMVGGVTPGKG
                     GTTHLGLPVFNTVREAVAATGATASVIYVPAPFCKDSILEAIDAGIKLIITITEGIPT
                     LDMLTVKVKLDEAGVRMIGPNCPGVITPGECKIGIQPGHIHKPGKVGIVSRSGTLTYE
                     AVKQTTDYGFGQSTCVGIGGDPIPGSNFIDILEMFEKDPQTEAIVMIGEIGGSAEEEA
                     AAYIKEHVTKPVVGYIAGVTAPKGKRMGHAGAIIAGGKGTADEKFAALEAAGVKTVRS
                     LADIGEALKTVLK"
     gene            complement(2304..3470)
                     /gene="sucC"
                     /locus_tag="J1Y83_001617"
     CDS             complement(2304..3470)
                     /gene="sucC"
                     /locus_tag="J1Y83_001617"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HBA7629769.1"
                     /translation="MNLHEYQAKQLFARYGLPAPVGYACTTPREAEEAASKIGAGPWV
                     VKCQVHAGGRGKAGGVKVVNSKEDIRAFAENWLGKRLVTYQTDANGQPVNQILVEAAT
                     DIAKELYLGAVVDRSSRRVVFMASTEGGVEIEKVAEETPHLIHKVALDPLTGPMPYQG
                     RELAFKLGLEGKLVQQFTKIFMGLATIFLERDLALIEINPLVITKQGDLICLDGKLGA
                     DGNALFRQPDLREMRDQSQEDPREAQAAQWELNYVALDGNIGCMVNGAGLAMGTMDIV
                     KLHGGEPANFLDVGGGATKERVTEAFKIILSDDKVKAVLVNIFGGIVRCDLIADGIIG
                     AVAEVGVNVPVVVRLEGNNAELGAKKLADSGLNIIAAKGLTDAAQQVVAAVEGK"
     gene            complement(3564..4781)
                     /gene="odhB"
                     /locus_tag="J1Y83_001618"
     CDS             complement(3564..4781)
                     /gene="odhB"
                     /locus_tag="J1Y83_001618"
                     /EC_number="2.3.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase complex
                     dihydrolipoyllysine-residue succinyltransferase"
                     /protein_id="HBA7629770.1"
                     /translation="MSSVDILVPDLPESVADATVATWHKKPGDAVVRDEVLVEIETDK
                     VVLEVPASADGILDAVLEDEGTTVTSRQILGRLREGNSAGKETSAKSEEKASTPAQRQ
                     QASLEEQNNDALSPAIRRLLAEHNLDACAIKGTGVGGRLTREDVEKHLAKAPAKESAP
                     AAAAPAAQPTLAARSEKRVPMTRLRKRVAERLLEAKNSTAMLTTFNEVNMKPIMDLRK
                     QYGEAFEKRHGIRLGFMSFYVKAVVEALKRYPEVNASIDGDDVVYHNYFDVSMAVSTP
                     RGLVTPVLRDVDTLGMADIEKKIKELAVKGRDGKLTVEDLTGGNFTITNGGVFGSLMS
                     TPIINPPQSAILGMHAIKDRPMAVNGQVEILPMMYLALSYDHRLIDGRESVGFLVTIK
                     ELLEDPTRLLLDV"
     gene            complement(4796..7597)
                     /gene="sucA"
                     /locus_tag="J1Y83_001619"
     CDS             complement(4796..7597)
                     /gene="sucA"
                     /locus_tag="J1Y83_001619"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HBA7629771.1"
                     /translation="MQNSALKAWLDSSYLSGANQSWIEQLYEDFLTDPDSVDANWRST
                     FQQLPGTGVKPDQFHSQTREYFRRLAKDASRYSSTISDPDTNVKQVKVLQLINAYRFR
                     GHQHANLDPLGLWQQDKVADLDPSFHDLTEADFQETFNVGSFASGKETMKLGELLEAL
                     KQTYCGPIGAEYMHITSTEEKRWIQQRIESGRATFNSEEKKRFLSELTAAEGLERYLG
                     AKFPGAKRFSLEGGDALIPMLKEMIRHAGNSGTREVVLGMAHRGRLNVLVNVLGKKPQ
                     DLFDEFAGKHKEHLGTGDVKYHMGFSSDFQTDGGLVHLALAFNPSHLEIVSPVVIGSV
                     RARLDRLDEPSSNKVLPITIHGDAAVTGQGVVQETLNMSKARGYEVGGTVRIVINNQV
                     GFTTSNPLDARSTPYCTDIGKMVQAPIFHVNADDPEAVAFVTRLALDFRNTFKRDVFI
                     DLVCYRRHGHNEADEPSATQPLMYQKIKKHPTPRKIYADKLEQEKVATLEDATEMVNL
                     YRDALDAGDCVVAEWRPMNMHSFTWSPYLNHEWDEEYPNKVEMKRLQELAKRISTVPE
                     AVEMQSRVAKIYGDRQAMAAGEKLFDWGGAENLAYATLVDEGIPVRLSGEDSGRGTFF
                     HRHAVIHNQSNGSTYTPLQHIHNGQGAFRVWDSVLSEEAVLAFEYGYATAEPRTLTIW
                     EAQFGDFANGAQVVIDQFISSGEQKWGRMCGLVMLLPHGYEGQGPEHSSARLERYLQL
                     CAEQNMQVCVPSTPAQVYHMLRRQALRGMRRPLVVMSPKSLLRHPLAVSSLEELANGT
                     FLPAIGEIDELDPKGVKRVVMCSGKVYYDLLEQRRKNNQHDVAIVRIEQLYPFPHKAM
                     QEVLQQFAHVKDFVWCQEEPLNQGAWYCSQHHFREVIPFGASLRYAGRPASASPAVGY
                     MSVHQKQQQDLVNDALNVE"
     gene            complement(7958..8674)
                     /locus_tag="J1Y83_001620"
     CDS             complement(7958..8674)
                     /locus_tag="J1Y83_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase iron-sulfur subunit"
                     /protein_id="HBA7629772.1"
                     /translation="MRLEFSIYRYNPDVDDAPRMQDYTLEAEEGRDMMLLDALIQLKE
                     KDPSLSFRRSCREGVCGSDGLNMNGKNGLACITPISALNQPGKKIVIRPLPGLPVIRD
                     LVVDMGQFYAQYEKIKPYLLNNGQNPPAREHLQMPEQREKLDGLYECILCACCSTSCP
                     SFWWNPDKFIGPAGLLAAYRFLIDSRDTETDSRLDGLSDAFSVFRCHSIMNCVSVCPK
                     GLNPTRAIGHIKSMLLQRNA"
     gene            complement(8690..10456)
                     /gene="sdhA"
                     /locus_tag="J1Y83_001621"
     CDS             complement(8690..10456)
                     /gene="sdhA"
                     /locus_tag="J1Y83_001621"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase flavoprotein subunit"
                     /protein_id="HBA7629773.1"
                     /translation="MKLPVREFDAVVIGAGGAGMRAALQISQSGQTCALLSKVFPTRS
                     HTVSAQGGITVALGNTHEDNWEWHMYDTVKGSDYIGDQDAIEYMCKTGPEAILELEHM
                     GLPFSRLDDGRIYQRPFGGQSKNFGGEQAARTAAAADRTGHALLHTLYQQNLKNHTTI
                     FSEWYALDLVKNQDGAVVGCTALCIETGEVVYFKARATVLATGGAGRIYQSTTNAHIN
                     TGDGVGMAIRAGVPVQDMEMWQFHPTGIAGAGVLVTEGCRGEGGYLLNKHGERFMERY
                     APNAKDLAGRDVVARSIMIEIREGRGCDGPWGPHAKLKLDHLGKEVLESRLPGILELS
                     RTFAHVDPVKEPIPVIPTCHYMMGGIPTKVTGQALTVNEKGEDVVVPGLFAVGEIACV
                     SVHGANRLGGNSLLDLVVFGRAAGLHLQESIAEQGALRDASESDVEASLDRLNRWNNN
                     RNGEDPVAIRKALQECMQHNFSVFREGDAMAKGLEQLKVIRERLKNARLDDTSSEFNT
                     QRVECLELDNLMETAYATAVSANFRTESRGAHSRFDFPDRDDENWLCHSLYLPESESM
                     TRRSVNMEPKLRPAFPPKIRTY"
     gene            complement(10456..10803)
                     /gene="sdhD"
                     /locus_tag="J1Y83_001622"
                     /gene_synonym="cybS"
                     /gene_synonym="dhsD"
     CDS             complement(10456..10803)
                     /gene="sdhD"
                     /locus_tag="J1Y83_001622"
                     /gene_synonym="cybS"
                     /gene_synonym="dhsD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase membrane anchor subunit"
                     /protein_id="HBA7629774.1"
                     /translation="MVSNASALGRNGVHDFILVRATAIVLTLYIIYMVGFFATSGELT
                     YEVWIGFFASAFTKVFTLLALFSILIHAWIGMWQVLTDYVKPLALRLMLQLVIVVALV
                     VYVIYGFVVVWGV"
     gene            complement(10797..11201)
                     /gene="sdhC"
                     /locus_tag="J1Y83_001623"
                     /gene_synonym="cybA"
                     /gene_synonym="dhsC"
     CDS             complement(10797..11201)
                     /gene="sdhC"
                     /locus_tag="J1Y83_001623"
                     /gene_synonym="cybA"
                     /gene_synonym="dhsC"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000263334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase cytochrome b556 subunit"
                     /protein_id="HBA7629775.1"
                     /translation="MWALFMIRNVKKQRPVNLDLQTIRFPVTAIASILHRVSGVITFV
                     AVGILLWLLGTSLSSPEGFEQASAIMGSFFVKFIMWGILTALAYHVVVGIRHMMMDFG
                     YLEETFEAGKRSAKISFVITVVLSLLAGVLVW"
     gene            11582..11689
                     /locus_tag="J1Y83_001624"
     CDS             11582..11689
                     /locus_tag="J1Y83_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA7629776.1"
                     /translation="MRKSYEVGISPKINLCNSVEVLTNSFGTVISGRQV"
     gene            11895..13178
                     /gene="gltA"
                     /locus_tag="J1Y83_001625"
     CDS             11895..13178
                     /gene="gltA"
                     /locus_tag="J1Y83_001625"
                     /EC_number="2.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate (Si)-synthase"
                     /protein_id="HBA7629777.1"
                     /translation="MADTKAKLTLNGDTAVELDVLKGTLGQDVIDIRTLGSKGVFTFD
                     PGFTSTASCESKITFIDGDEGILLHRGFPIDQLATDSNYLEVCYILLNGEKPTQEQYD
                     EFKTTVTRHTMIHEQITRLFHAFRRDSHPMAVMCGITGALAAFYHDSLDVNNPRHREI
                     AAFRLLSKMPTMAAMCYKYSIGQPFVYPRNDLSYAGNFLNMMFSTPCEPYEVNPILER
                     AMDRILILHADHEQNASTSTVRTAGSSGANPFACIAAGIASLWGPAHGGANEAALKML
                     EEISSVKHIPEFVRRAKDKNDSFRLMGFGHRVYKNYDPRATVMRETCHEVLKELGTKD
                     DLLEVAMELENIALNDPYFIEKKLYPNVDFYSGIILKAMGIPSSMFTVIFAMARTVGW
                     IAHWSEMHSDGMKIARPRQLYTGYEKRDFKSDIKR"
     gene            13324..13698
                     /locus_tag="J1Y83_001626"
     CDS             13324..13698
                     /locus_tag="J1Y83_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001519567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HBA7629778.1"
                     /translation="MVDFGKFNLLDIRRHTMSKTFSIKTTKSQSDQCTDGFKVSSSFY
                     TEKTLVEEDKALLIGNGLKLRILDENASPYTFNKYAEYADFTSDMLIYEKTYTAELSS
                     TPGTPIAAGPFDTVVLFKINYN"
     gene            13850..14896
                     /locus_tag="J1Y83_001627"
     CDS             13850..14896
                     /locus_tag="J1Y83_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415243.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HBA7629779.1"
                     /translation="MPVLQWGMLCVLSLLLSIGFLALHLPAALLLGPMIAGIIFSMRG
                     VTLQLPRSAFLAAQAILGCMIAQNLTGSILTTLAVNWPIVLAILLVTLLSSAIVGWLL
                     VRYSSLPGNTGAWGSSPGGAAAMVAMAQDYGADIRLVAFMQYLRVLFVAGAAVLVTRM
                     MLGDNAEAVNQQIVWFPPVSINLLLTILLAVVAGTAGCLLRLPSGTMLIPMLAGAVLQ
                     SGQLITIELPEWLLAMAYMAIGWRIGLGFDKQILLRALRPLPQILLSIFALLAICAGM
                     AWGLTRFMHIDFMTAYLATSPGGLDTVAVIAAGSNADMALIMAMQTLRLFSILLTGPA
                     IARFISTYAPKRSA"
     gene            complement(14893..15684)
                     /gene="nei"
                     /locus_tag="J1Y83_001628"
     CDS             complement(14893..15684)
                     /gene="nei"
                     /locus_tag="J1Y83_001628"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease VIII"
                     /protein_id="HBA7629780.1"
                     /translation="MPEGPEIRRAADNLEAAIKGKPLTDVWFAFPQLKSYQSRLIGQH
                     VTHVETRGKALLTHFSNDLTLYSHNQLYGVWRVVDTGEEPQTTRVLRVKLQTADKTIL
                     LYSASDIEMLTPEQLTTHPFLQRVGPDVLDPNLTPEVVKERLLSPRFRNRQFAGLLLD
                     QAFLAGLGNYLRVEILWQVGLTGNHKAKDLNAAQLDALAHALLDIPRLSYATRGQVDE
                     NKYHGALFRFKVFHRDGEPCERCGGIIEKTTLSSRPFYWCPGCQH"
     gene            complement(15720..16454)
                     /gene="pxpA"
                     /locus_tag="J1Y83_001629"
     CDS             complement(15720..16454)
                     /gene="pxpA"
                     /locus_tag="J1Y83_001629"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpA"
                     /protein_id="HBA7629781.1"
                     /translation="MKIDLNADLGEGCASDAELLTLVSSANIACGFHAGDAQTMQACV
                     REAIKNGVAIGAHPSFPDRENFGRSAMQLPPETVFAQTLYQIGALAAITRAQGGVMCH
                     VKPHGMLYNQAAKEAQLADAIARAVYACDPALILVGLAGSELIRAGERYGLVTREEVF
                     ADRGYQADGSLVPRSQPGALIENEEQALAQTLEMVQHGRVKSITGEWAMVTAQTVCLH
                     GDGEHALAFARRLRATFAEKGIVVAA"
     gene            complement(16444..17376)
                     /gene="pxpC"
                     /locus_tag="J1Y83_001630"
     CDS             complement(16444..17376)
                     /gene="pxpC"
                     /locus_tag="J1Y83_001630"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpC"
                     /protein_id="HBA7629782.1"
                     /translation="MLKIIRAGMYTTVQDGGRHGFRQSGISHCGALDMPALRIANLLV
                     GNDANAPALEITLGQLTVEFETDGWFALTGAGCEARLDDNAVWTGWRLPMRAGQRLTL
                     KRPQHGMRSYLAVAGGIDVPPVMGSCSTDLKVGIGGLEGRLLRDGDRLPIGKAKRDFM
                     EAQGVKQLLWGNRIRALPGPEYHEFDRASQDAFWRSPWQLSSQSNRMGYRLQGQILKR
                     TTDRELLSHGLLPGVVQVPHNGQPIVLMNDAQTTGGYPRIACIIEADMYHLAQIPLGQ
                     PIHFVQCSLEEALKARQDQQRYFEQLAWRLHNEN"
     gene            complement(17370..18026)
                     /gene="pxpB"
                     /locus_tag="J1Y83_001631"
     CDS             complement(17370..18026)
                     /gene="pxpB"
                     /locus_tag="J1Y83_001631"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpB"
                     /protein_id="HBA7629783.1"
                     /translation="MQRARCYLIGETAVVLELDPPVTLASQKRIWRLAQRLVNMPNVV
                     EAIPGMNNITVILRNPESLALDAIERLQRWWEESEALEPESRFIEIPVVYGGAAGPDL
                     AVVAAHCGLSEKQVVELHSSVEYVVWFLGFQPGFPYLGSLPEQLHTPRRAEPRLHVPA
                     GSVGIGGPQTGVYPLATPGGWQLIGHTSLSLFDPERDEPILLRPGDSVRFVPQKEGVC
                     "
     gene            complement(18049..18792)
                     /gene="ybgI"
                     /locus_tag="J1Y83_001632"
     CDS             complement(18049..18792)
                     /gene="ybgI"
                     /locus_tag="J1Y83_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000798886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radiation resistance protein YbgI"
                     /protein_id="HBA7629784.1"
                     /translation="MKNTELEQLINEKLNSAAISDYAPNGLQVEGKETVQKIVTGVTA
                     SQALLDEAVRLGADAVIVHHGYFWKGESPVIRGMKRNRLKTLLANDINLYGWHLPLDA
                     HPELGNNAQLAALLGITVMGEIEPLVPWGELTMPVPGLELASWIEARLGRKPLWCGDT
                     GPEVVQRVAWCTGGGQSFIDSAARFGVDAFITGEVSEQTIHSAREQGLHFYAAGHHAT
                     ERGGIRALSEWLNENTDLDVTFIDIPNPA"
     gene            19063..20544
                     /gene="dtpD"
                     /locus_tag="J1Y83_001633"
     CDS             19063..20544
                     /gene="dtpD"
                     /locus_tag="J1Y83_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide permease DtpD"
                     /protein_id="HBA7629785.1"
                     /translation="MNKHASQPRAIYYVVALQIWEYFSFYGMRALLILYLTNQLKYSD
                     NHAYELFSAYCSLVYVTPILGGFLADKVLGNRMAVMLGALLMAIGHVVLGASEIHPSF
                     LYLSLAIIVCGYGLFKSNVSCLLGELYEPTDPRRDGGFSLMYAAGNVGSIIAPIACGF
                     AQEEYSWAMGFGLAAVGMIAGLVIFLCGNRHFTHTRGVNKKVLRATNFLLPNWGWLLV
                     LLVATPALITVLFWKEWSVYALIVATIIGLGVLAKIYRKAENQKQRKELGLIVTLTFF
                     SMLFWAFAQQGGSSISLYIDRFVNRDMFGYTVPTAMFQSINAFAVMLCGVFLAWVVKE
                     SVAGNRTVRIWGKFALGLGLMSAGFCILTLSARWSAMYGHSSLPLMVLGLAVMGFAEL
                     FIDPVAMSQITRIEIPGVTGVLTGIYMLLSGAIANYLAGVIADQASQASFDASGAINY
                     SINAYIEVFDQITWGALACVGLVLMIWLYQALKFRNRALALES"
     gene            complement(20586..22004)
                     /gene="phrB"
                     /locus_tag="J1Y83_001634"
     CDS             complement(20586..22004)
                     /gene="phrB"
                     /locus_tag="J1Y83_001634"
                     /EC_number="4.1.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribodipyrimidine photo-lyase"
                     /protein_id="HBA7629786.1"
                     /translation="MTTHLVWFRQDLRQHDNLALAAACRNSSARVLALYIATPRQWAA
                     HNVSPRQAELINTQLNALQNALAEKGIPLLFREVDDFAASVEIVKQVCAEYRVTHLFY
                     NYQYEVNERARDVQAERTLRNVVCEGFDDSVILPPGAVMTGNHEMYKVFTPFKNAWLK
                     RLREGMPECVAAPKVRSSGSIEPAPSITLNYPRQSFDTAHFPVEEKAAIAQLRQFCLN
                     GAGEYEQQRDFPAVEGTSRLSASLATGGLSPRQCLHRLLAEQPQVLEGGPGSVWLNEL
                     IWREFYRHLMTYYPSLCKHRPFIAWTDRVQWQSNPAHLKAWQEGKTGYPIVDAAMRQL
                     NSTGWMHNRLRMITASFLVKDLLIDWREGERYFMSQLIDGDLAANNGGWQWAASTGTD
                     AAPYFRIFNPTTQGEKFDREGEFIRQWLPELRDVPGKAVHEPWKWAEKTGVTLDYPQP
                     IVDHKEARLRTLAAYEEARKGA"
     gene            complement(22001..22510)
                     /locus_tag="J1Y83_001635"
     CDS             complement(22001..22510)
                     /locus_tag="J1Y83_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbgA family protein"
                     /protein_id="HBA7629787.1"
                     /translation="MNQQLFDDSTIIRIFALHELHNLKDRGLTRGALLDYHSRYKLVF
                     LAHSQPEYRKLGPFVADIHRWQSLDDYYNQYRQRVIVLLSHPANRRDQTNVLMHVQGY
                     FRPHIDSTERQQLAALIDSYRRGEQPLLAPLMRIKHYMALYPDAWLSGQRYFELWPRV
                     INLRHAGVL"
     gene            complement(22611..22817)
                     /locus_tag="J1Y83_001636"
     CDS             complement(22611..22817)
                     /locus_tag="J1Y83_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbfA family protein"
                     /protein_id="HBA7629788.1"
                     /translation="MELYKEYPAWLIFLRRTCAVAAGVLALPFMLFWKDRARFYSYLH
                     RVWSKTSDKPVWMDQAEKATGDFY"
     gene            23130..23219
                     /gene="kdpF"
                     /locus_tag="J1Y83_001637"
     CDS             23130..23219
                     /gene="kdpF"
                     /locus_tag="J1Y83_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit F"
                     /protein_id="HBA7629789.1"
                     /translation="MSAGVITGVLLVFLLLGYLVYALINAEAF"
     gene            23219..24892
                     /gene="kdpA"
                     /locus_tag="J1Y83_001638"
     CDS             23219..24892
                     /gene="kdpA"
                     /locus_tag="J1Y83_001638"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpA"
                     /protein_id="HBA7629790.1"
                     /translation="MAAQGFLLIATFLLVLMVLARPLGSGLARLINDIPLPGTTGVER
                     VLFSALGVSDREMNWKQYLSAILGLNILGLAVLFFMLLGQHYLPLNPQQLPGLSWDLA
                     LNTAVSFVTNTNWQSYSGETTLSYFSQMAGLTVQNFLSAASGIAVIFALIRAFTRQSM
                     NTLGNAWVDLLRITLWVLTPVALLIALFFIQQGALQNFLPYQAVTTIEGAQQLLPMGP
                     VASQEAIKMLGTNGGGFFNANSSHPFENPTALTNFVQMLAIFLIPTALCFAFGEVAGD
                     RRQGRMLLWAMSVIFVICVGVVMWAEVQGNPHLLALGADSSINMEGKESRFGVLVSSL
                     FAVVTTAASCGAVIAMHDSFTALGGMVPMWLMQIGEVVFGGVGSGLYGMMLFVLLAVF
                     IAGLMIGRTPEYLGKKIDVREMKLTALAILVTPTLVLMGAALAMMTDAGRSAMLNPGP
                     HGFSEVLYAVSSAANNNGSAFAGLSANSPFWNCLLALCMFVGRFGVIIPVMAIAGSLV
                     SKKSQPASSGTLPTHGPLFVGLLIGTVLLVGALTFIPALALGPVAEYLS"
     gene            24915..26963
                     /gene="kdpB"
                     /locus_tag="J1Y83_001639"
     CDS             24915..26963
                     /gene="kdpB"
                     /locus_tag="J1Y83_001639"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpB"
                     /protein_id="HBA7629791.1"
                     /translation="MSRKQLALFEPTLVVQALKEAVKKLNPQAQWRNPVMFIVWIGSL
                     LTTCISIAMASGVMPGNALFSAAISGWLWVTVLFANFAEALAEGRSKAQANSLKGVKK
                     TAFARKLREPKYGAAADKVPADQLRKGDIVLVEAGDIIPCDGEVIEGGASVDESAITG
                     ESAPVIRESGGDFASVTGGTRILSDWLVIECSVNPGETFLDRMIAMVEGAQRRKTPNE
                     IALTILLIALTIVFLLATATLWPFSAWGGNAVSVTVLVALLVCLIPTTIGGLLSAIGV
                     AGMSRMLGANVIATSGRAVEAAGDVDVLLLDKTGTITLGNRQASEFIPAQGVEEKALA
                     DAAQLASLADETPEGRSIVILAKQRFNLRERDVQSLHATFVPFTAQSRMSGINIDNRM
                     IRKGSVDAIRRHVEANGGHFPADVDQKVDQVARQGATPLVVVEGSRVLGVIALKDIVK
                     GGIKERFAQLRKMGIKTVMITGDNRLTAAAIAAEAGVDDFLAEATPEAKLALIRQYQA
                     EGRLVAMTGDGTNDAPALAQADVAVAMNSGTQAAKEAGNMVDLDSNPTKLIEVVHIGK
                     QMLMTRGSLTTFSIANDVAKYFAIIPAAFAATYPQLNALNIMRLHSPDSAILSAVIFN
                     ALIIVFLIPLALKGVSYKPLTASAMLRRNLWIYGLGGLLVPFIGIKVIDLLLTVCGLV
                     "
     gene            26972..27544
                     /gene="kdpC"
                     /locus_tag="J1Y83_001640"
     CDS             26972..27544
                     /gene="kdpC"
                     /locus_tag="J1Y83_001640"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit C"
                     /protein_id="HBA7629792.1"
                     /translation="MRGLRPALSTFLFLLLITGGVYPLLTTALGQWWFPWQANGSLIR
                     EGDTVRGSALIGQNFTGNGYFHGRPSATAEMPYNPQASGGSNLAVSNPELDKQIAARV
                     AALRAANPDASTNVPVELVTASASGLDNNITPQAAAWQIPRVAKARNLSVEQLTQLIA
                     KYSQQPLVKYIGQPVVNIVELNLALDKLDE"
     gene            27537..30221
                     /gene="kdbD"
                     /locus_tag="J1Y83_001641"
     CDS             27537..30221
                     /gene="kdbD"
                     /locus_tag="J1Y83_001641"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     KdbD"
                     /protein_id="HBA7629793.1"
                     /translation="MNNEPLRPDPDRLLEQAAAPHRGKLKVFFGACAGVGKTWAMLAE
                     AQRLRAQGLDIVAGVVETHGRKDTAAMLKGLAVLPLKRQAYRGRHISEFDLDAALARR
                     PALILMDELAHSNAPGSRHPKRWQDIEELLEAGIDVFTTVNVQHLESLNDVVSGVTGI
                     QVRETVPDPFFDAADDVVLVDLPPDDLRQRLKEGKVYIAGQAERAIEHFFRKGNLIAL
                     RELALRRTADRVDEQMRAWRGHPGEEKVWHTRDAILLCIGHNTGSEKLVRAAARLASR
                     LGSVWHAVYVETPALHRLPEKKRRAILSALRLAQELGAETATLSDPAEEKAVVRYARE
                     HNLGKIILGRPASRRWWRRETFADRLARIAPDLDQVLVALDEPPARTINNAPDSRSFK
                     DKWRVQIQGCVVAAALCAVITLIAMQWLMAFDAANLVMLYLLGVVVVALFYGRWPSVV
                     ATVINVVSFDLFFIAPRGTLAVSDVQYLLTFAVMLTVGLVIGNLTAGVRYQARVARYR
                     EQRTRHLYEMSKALAVGRSSQDIAATSEQFIASTFHARSQVLLPDDNGKLQPLTHPQG
                     MTPWDDAIAQWSFDKGLPAGAGTDTLPGVPYQILPLKSGEKTYGLVVVEPGNLRQLMI
                     PEQQRLLETFTLLVANALERLTLTASEEQARMASEREQIRNALLAALSHDLRTPLTVL
                     FGQAEILTLDLASEGSPHARQASEIRQHVLNTTRLVNNLLDMARIQSGGFNLKKEWLT
                     LEEVVGSALQMLEPGLSSPINLSLPEPLTLIHVDGPLFERVLINLLENAVKYAGAQAE
                     IGINAHVEGENLQLDVWDNGPGLPPGQEQTIFDKFARGNKESAVPGVGLGLAICRAIV
                     DVHGGTITAFNRPEGGACFRVTLPQQTAPELEEFHEDM"
     gene            30218..>30845
                     /gene="kdpE"
                     /locus_tag="J1Y83_001642"
     CDS             30218..>30845
                     /gene="kdpE"
                     /locus_tag="J1Y83_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator KdpE"
                     /protein_id="HBA7629794.1"
                     /translation="MTNVLIVEDEQAIRRFLRTALEGDGMRVFEAETLQRGLLEAATR
                     KPDLIILDLGLPDGDGIEFIRDLRQWSAVPVIVLSARSEESDKIAALDAGADDYLSKP
                     FGIGELQARLRVALRRHSATTTPDPLVKFSDVTVDLAARVIHRGEEEVHLTPIEFRLL
                     AVLLNNAGKVLTQRQLLNQVWGPNAVEHSHYLRIYMGHLRQKLEQDPAR"
BASE COUNT         6962 a         8340 c         8035 g         7508 t
ORIGIN      
        1 aaacagtgaa tgatggataa cttcaccaga aaaacaagca agcatataaa atttaaaaat
       61 gtgattccac tttcattttt ataaaatggc atcgtgaaga gcgagttgat aatgctgcaa
      121 tgcatcagcg tcctgtccag cagtttcata aattttggca atgttataat aggagttagc
      181 gcgcgttgtc gccgcatttg ctccactggc caacgagatc gctttacgat ttgcccaaat
      241 tgcttccgcg atgttgccat ttttctgata cgccagcccc aggttactaa atgcctgtcc
      301 gtaattgccg tctaattctg ttgcctgttg gaagagttga atggcttgct caagttgacc
      361 ggttttataa acagcttcac cttcgcgatt atattttctt gccagttgag tatctccact
      421 gttttgcatc gcaggcgaag ccggagctga agtgcccgtc gcaggcatta tcacagtggc
      481 taaatccctg acgtctaatg tggtgtcgtt aaaattagcg ctggtaacag agttaatatc
      541 ttcaaactcg ccgtttggat tgagtttaaa cacggtccag atgttccctc gttttcccgc
      601 agggacacta taactacgca ccagggagct ccctacataa acaaacactt ttgccttact
      661 ggcagagaga taattactgt tgggtaaccc tttattgcta tagtcctgca cggcatagat
      721 gtaactttcg ccaaaatgct ttttgctgat agtgacagtt tctggtccat agctatccgt
      781 atcatcaaca tccagattag catcagtccc ctctttaact atcaaaatag atatgtccgc
      841 cagggaagat caaatgtgaa tcaagatcaa aaggcttttc gccccagctc aaaactacac
      901 gcatgccatc cagactagtc attaccggac taatcgcata agtcatgcca gcacacggac
      961 atttgaccac cagattagaa tagccctctt ttttgacgat caataaagca tcttgatcat
     1021 cagcaaatgt agaacccaga ttaacgctgc ccgatgcatt tgtggttcca gaaacagatt
     1081 gtgcgccatt gtgttgcagc gtcaccgatg ctccctcaat gcgtttatct ttcactgtcg
     1141 cacttaaaat atcgatattt acatcggccg cataaacgtc cgaatttata cccatcaggg
     1201 ccagagcaac cggcagaaca attttcttct tgtataaatg cttttacaga acggagaggg
     1261 gatattttgc cgggcattat tgcgcagaag atgggaaaaa agcagtgggc aaacttttca
     1321 gattagtgcc cgaataaaaa aggccatcat aagatggcca accatgtcga aaccgacagc
     1381 ccttcgcccc tcctgtagag ggaagcggcg agggctattt cttattacag atatttattt
     1441 cagaacagtt ttcagtgctt caccgatatc cgccaggctg cgaacggttt tcacgcctgc
     1501 ggcttccaga gcagcgaatt tctcatcagc agtcccttta ccaccggcaa tgatcgcacc
     1561 tgcgtgaccc atacgtttgc ctttcggcgc agtcacacca gcgatgtaac ccacaactgg
     1621 cttggtaacg tgctccttga tgtacgccgc tgcttcttct tcagcgctac cgccgatctc
     1681 accgatcatc acgatcgctt cggtctgcgg atctttttcg aacatttcga ggatgtcgat
     1741 aaagttagag ccagggatcg ggtcgccgcc gataccaaca caggtcgact gaccgaaacc
     1801 gtaatccgtg gtctgtttaa ccgcttcata ggtcagcgta ccggaacggg aaacgatacc
     1861 cactttacct ggtttgtgaa tgtgacccgg ctggataccg attttgcatt cccccggggt
     1921 gataacgcct gggcagttcg ggccgatcat acgaacgcct gcttcatcca gtttcacttt
     1981 caccgtcagc atatccagcg tcgggatgcc ttcagtgatg gtgataatca gtttgatgcc
     2041 tgcatcgata gcttccagaa tggagtcttt gcagaacggt gctggtacgt agataacaga
     2101 ggcggtagcg ccagtagcag caacagcttc acgcacggtg ttgaacaccg gcaggccgag
     2161 gtgggtagta ccgcctttac ctggggttac gccgccaacc attttagtgc cgtatgcaat
     2221 ggcctgttct gagtggaaag tcccctggct accggtaaag ccctggcaga taaccttggt
     2281 gtttttatca attaaaatgg acattatttc ccctccactg cggcaacaac ctgctgagct
     2341 gcatccgtca gaccttttgc tgcaataata ttcaggccgc tgtcagccag tttcttcgca
     2401 ccgagttcgg cgttgttacc ttccagacgt accacgaccg gtacgttaac acccacttct
     2461 gctaccgcgc cgatgatacc gtcagcgatc aggtcgcaac gaacgatacc gccgaagatg
     2521 ttaaccagaa cggctttcac tttgtcgtca gagaggatga ttttgaacgc ttcggttaca
     2581 cgttctttgg ttgcgccgcc gccaacgtcc aggaagttag ccggttcgcc gccgtgcagt
     2641 ttaacgatgt ccatcgtacc catcgccaga cctgcgccgt taaccataca accgatgtta
     2701 ccgtccagcg caacgtagtt cagttcccac tgtgcagcct gtgcttcacg cggatcttcc
     2761 tgcgactggt cacgcatttc acgcagatca ggctggcgga acagtgcgtt gccatcagcg
     2821 cccagtttgc cgtcgaggca aatcagatcg ccctgtttgg taataaccag cgggttgatt
     2881 tcgataagcg caaggtcgcg ctccaggaaa atggtcgcca ggcccatgaa gattttggtg
     2941 aactgctgaa ccagtttacc ttccagaccc agtttaaacg ccagttcgcg tccctgatac
     3001 ggcatcgggc cagtcagcgg atcaagcgca actttgtgga tcaggtgcgg agtttcttcc
     3061 gccacttttt cgatttccac gccgccttcg gtggaggcca taaagaccac gcgacgggaa
     3121 ctacggtcaa caacggcacc gagatacagc tctttagcga tatcggtcgc tgcttcaacc
     3181 agaatctggt taaccggttg gccattagca tctgtttgat acgttaccag acgcttgccc
     3241 agccagtttt ctgcaaaagc acggatgtct tctttgctgt ttacaacttt cacaccgccc
     3301 gctttaccgc ggccaccagc gtgaacctga catttcacta cccacggacc ggcaccgatt
     3361 tttgaagcgg cttcttctgc ttcgcgcgga gtagtacagg cataacccac cggtgctggt
     3421 aagccatagc gggcaaaaag ttgttttgcc tgatattcat gtaagttcat gtgttctgtc
     3481 catccttcag taatcgttat cttttaaacc gtagaccgac cccctatcca cgtcggtcta
     3541 cagtgcaggt gaagcttaaa ctactaaacg tccagcagca gacgcgtcgg atcttccagc
     3601 aactctttga tcgttacaag gaagcccacg gattcgcgac catcgatcaa acggtgatcg
     3661 taggacagcg ccaggtacat catcggcagg atctcaacct gaccattcac tgccatcgga
     3721 cgatctttga tagcgtgcat acccagaatt gcgctctgcg gtgggttgat gataggcgtt
     3781 gacatcaggg aaccgaacac accaccgttg gtgatggtga agttaccacc ggtcagatct
     3841 tcaaccgtca gcttgccgtc acggccttta acagccagct ctttgatttt cttctcgatg
     3901 tctgccatgc cgagggtatc gacatcacgc agaaccggcg tcaccaggcc gcgcggcgta
     3961 gaaaccgcca tactgacgtc gaaatagttg tggtagacca catcatcacc gtcgatagac
     4021 gcattcactt ccgggtaacg tttcagggct tcaaccaccg ctttcacgta gaaggacata
     4081 aagcccagac ggatgccgtg gcgtttttca aacgcttcac cgtactgctt acgcagatcc
     4141 ataatcggct tcatgttgac ttcgttgaac gtggtcagca tggcggtgga gtttttcgct
     4201 tccagcagac gctctgccac acgcttacgc aggcgagtca tcggcacacg tttttcgcta
     4261 cgtgcagcca gggtcggttg cgccgccgga gcagccgctg ccggagcaga ctctttcgcc
     4321 ggggctttcg ccagatgttt ttccacatct tcacgggtca gacgaccacc cacacccgtg
     4381 cctttaatgg cgcaggcgtc gaggttatgt tcagccagca ggcgacggat cgccgggctt
     4441 aacgcatcgt tgttttgctc ttccagagac gcctgctggc gttgcgccgg agtggacgct
     4501 ttctcttcag atttggcgct ggtttcttta cccgcgctgt tgccttcacg caggcgacca
     4561 aggatctgac gagacgttac cgttgtacct tcatcttcca gaaccgcatc cagaatgccg
     4621 tctgctgatg ccggtacttc cagtaccact ttgtcagttt cgatttctac cagcacttca
     4681 tcacgtacga ctgcgtcgcc gggtttttta tgccaggttg cgacggtggc atcggctacg
     4741 gattcaggca ggtcagggac cagaatatct acgctactca ttgtgtatcc tttatttatt
     4801 cgacgttcag cgcgtcatta accagatctt gttgctgttt ctggtgaacg gacatatacc
     4861 ctaccgccgg agaggcggag gctgggcggc ctgcataacg cagagaagcc ccaaacggaa
     4921 tcacttcacg gaaatgatgc tggctgcagt accatgcgcc ctggttgagc ggctcttcct
     4981 ggcaccagac aaaatccttg acgtgagcaa actgctgcaa cacttcctgc atcgctttat
     5041 gcgggaacgg gtagagttgc tcgatacgca caatggcgac atcgtgttga ttgttcttac
     5101 gacgttgttc cagcaggtcg taataaacct taccagaaca catcactacg cgcttcacgc
     5161 ccttcggatc aagctcgtcg atttcaccga tggctggcag gaaggtgccg ttcgccagtt
     5221 cttcgaggct ggataccgcc agcggatgac gcagcaggga tttcggcgac atcacgacca
     5281 gtggacgacg catcccgcgc aacgcctgac gacgcagcat gtggtaaacc tgtgccgggg
     5341 tagaaggtac gcaaacctgc atgttttgct cagcgcaaag ttgcagataa cgttccagac
     5401 gcgcggagga gtgctccggc ccctgccctt cgtaaccgtg cggcagcaac atcaccaggc
     5461 cacacatccg gccccatttc tgttcgccag aggaaatgaa ctggtcgata accacctgtg
     5521 caccgttggc gaagtcaccg aactgtgctt cccagatggt cagggtgcgt ggttctgcgg
     5581 tggcataacc gtattcaaac gccagtacgg cttcttcaga cagtacagag tcccagacgc
     5641 ggaacgcgcc ctggccgtta tggatatgtt gcagcggcgt gtaagtggaa ccgttagact
     5701 ggttgtggat caccgcgtgg cggtggaaga aggtaccgcg accggagtct tcacccgaca
     5761 ggcgaaccgg aatgccttcg tcaaccaacg tggcgtaagc gaggttttcc gcgccgcccc
     5821 agtcgaacag tttctcaccg gcagccattg cctggcgatc gccataaatc ttggcaacgc
     5881 gagactgcat ttcaaccgct tccggcaccg tgctgatgcg tttagccagt tcctgcaggc
     5941 gcttcatctc aactttgttc gggtactctt cgtcccattc atggttgagg tacggcgacc
     6001 aggtgaaaga gtgcatgttc atcggacgcc actctgctac aacgcaatcg ccagcatcca
     6061 gcgcatcgcg gtacaggtta accatctcgg tggcatcttc cagcgtcgcg actttttcct
     6121 gctccagctt gtcagcgtag attttgcgcg gcgtcggatg ttttttgatt ttctgataca
     6181 tcagcggctg ggttgcgctc ggctcgtcgg cttcgttgtg gccgtgacgg cggtagcata
     6241 ccaggtcgat gaagacatca cgtttaaagg tgttacggaa atcgagcgcc agacgggtaa
     6301 caaaggcaac ggcttccgga tcatccgcgt taacgtggaa aatcggtgcc tgaaccatct
     6361 taccgatatc agtacagtac ggcgtcgaac gggcatccag cgggttagag gtggtgaagc
     6421 caacctggtt gttgataacg atacgtaccg taccgccaac ttcataacca cgcgctttcg
     6481 acatgttcag ggtttcctga accacgccct gcccggtcac tgcggcgtca ccatgaatgg
     6541 tgattggcag cactttgttg ctgctcggct catcaagtct gtccagacgg gcacgaacag
     6601 aaccgataac gaccgggctt acaatctcaa ggtgagacgg gttaaacgcc agcgccaggt
     6661 gaaccaggcc gccatcggtc tggaagtcag acgagaagcc catgtggtat ttcacgtcgc
     6721 ccgtgccgag gtgttcttta tgtttaccgg caaactcgtc gaacaagtct tgcggttttt
     6781 tacccagcac gttcaccagc acgttcagac gaccacggtg cgccattccg agaaccactt
     6841 cgcgggtgcc gctgttgcca gcgtggcgga tcatctcttt aagcatcggg attaacgcgt
     6901 caccgccttc cagcgagaag cgttttgcgc cagggaattt tgcgccgagg taacgttcaa
     6961 ggccttcagc ggcggtcagt tcgcttaaga agcgtttttt ctcttcgcta ttgaaagtcg
     7021 cgcgaccaga ctcaatacgc tgttggatcc agcgtttttc ttcagtgctg gtaatgtgca
     7081 tatactcggc accaatcggg ccgcagtagg tttgcttgag tgcttccagc agttcgccga
     7141 gtttcatggt ttctttgccg ctggcaaatg aaccgacgtt gaaggtctcc tggaagtctg
     7201 cttcggtcag atcgtggaaa gacggatcca gatcggccac tttatcttgc tgccacagtc
     7261 ccagcggatc gagattcgca tgctggtgac cacggaagcg gtatgcgtta atgagctgca
     7321 ggactttaac ctgcttcaca ttggtgtcag ggtcggagat cgttgaagag taacgtgaag
     7381 cgtctttcgc caggcggcgg aaatattcac gcgtttgaga gtggaattga tccggtttga
     7441 ctcccgtacc aggtaactgc tggaacgtcg aacgccagtt agcgtcaacc gagtcaggat
     7501 cggttaagaa gtcttcatag agctgttcta tccagctctg gtttgcgcca gagaggtaag
     7561 aagagtccaa ccaggctttc aaagcgctgt tctgcatcgt gatcccttaa gcatcttttt
     7621 tatgcttact tcgccgtgga tactaccacg cacatttcat atctgatgtg cgtgccgggg
     7681 ttcacttgtt gcggacttgt cgtgttttct gcgtccgcta aggaaccttt aaaaactgtc
     7741 ttagtgtcag gggcagtttt taaagatttc ctgcatttct tttgtaggcc ggataaggca
     7801 ttcacgccgc atccggcgct gtttgcctga tgcgacgctt gcgcgtctta tcaggcctac
     7861 ggtttctgca tttcttttgt aggccggata aggcgttcac gccgcatccg gcactggttg
     7921 cctgatgcga cgcttgcgcg tcttatcagg cctgggttta cgcattacgt tgcaacaaca
     7981 tcgacttgat atggccgatg gcgcgcgtcg ggttcagccc cttcggacat acactgacgc
     8041 agttcatgat gctgtgacag cggaatacac tgaatgcatc gctcaaaccg tcgaggcggc
     8101 tgtcagtctc ggtatcacgg ctatcgatca ggaaacgata tgccgctaac aagcctgccg
     8161 ggccgataaa cttatcggga ttccaccaga aagacggaca agaggttgaa caacatgcgc
     8221 agagaataca ttcatacaac ccgtcgagtt tttcgcgctg ctctggcatc tgtaaatgct
     8281 cgcgagctgg cggattttgt ccattattca acaggtaagg cttaattttc tcatattgcg
     8341 catagaattg tcccatgtct accaccaaat cgcggatcac cggtaaacct ggcagcgggc
     8401 gaatcacaat cttcttgccc ggctggttga gtgccgaaat cggagtaata caggccagac
     8461 cattcttacc gttcatgttc agaccgtcgg aaccgcacac accttcacgg caggagcggc
     8521 ggaacgacag gctgggatct ttctctttca gctggataag cgcatccagc agcatcatgt
     8581 cacgaccttc ttccgcttcc agggtgtaat cctgcatacg cggagcatca tcaacatccg
     8641 ggttatagcg ataaattgaa aactcgagtc tcattttcct gtctccgcat tagtaagtac
     8701 gaatcttcgg cgggaatgcc gggcgcagtt tcggttccat gttgacgctt cggcgcgtca
     8761 tggattccga ctctggcaga tacagggagt ggcacagcca gttttcatca tcacgatccg
     8821 ggaagtcgaa gcggctatgc gcgccacggc tttcggtgcg gaagttggca gaaacagccg
     8881 ttgcatacgc cgtttccatc aggttatcca gttccaggca ctcaacgcgc tgggtgttga
     8941 actcgctgga agtgtcatcc agacgggcat ttttcagacg ctcgcggata actttcaact
     9001 gctcaagccc tttcgccatc gcatcacctt cacggaagac cgagaagtta tgctgcatac
     9061 attcttgcag tgctttacgg atcgccaccg gatcttcacc gttacggtta ttgttccagc
     9121 ggttcaggcg atccagagac gcttcaacat cagactcgct ggcatcgcgc agtgcgccct
     9181 gctcggcgat agactcttgc agatgcagac ctgccgcacg accaaatacg accaggtcca
     9241 gcagcgagtt gccgcccaga cggttagcgc catgtaccga tacacaagcg atttcaccaa
     9301 cggcaaacag ccccggaaca accacatctt cgcctttctc attcacagtc agcgcctgac
     9361 cggtcacttt ggtcggaata ccgcccatca tgtagtgaca ggttgggata accggaatcg
     9421 gctctttcac cggatcgacg tgagcgaagg tacgggagag ttcaaggata cccggcagac
     9481 gggattcgag aacttctttc cccagatggt caagtttcag ttttgcgtgt ggcccccacg
     9541 gaccatcaca gccgcggcct tcacggattt cgatcatgat ggaacgcgcc accacgtcac
     9601 ggcccgccag gtctttggcg ttcggcgcat aacgctccat aaagcgttcg ccatgtttgt
     9661 tcagcagata accgccttca ccacggcaac cttcggtgac cagtacgccc gcaccggcaa
     9721 taccggtcgg gtggaactgc cacatttcca tatcctgtac cggtacgcct gcacggatag
     9781 ccatgccgac accgtcgcca gtgttaatgt gggcgttggt ggtggactga taaatacgcc
     9841 ctgccccgcc agtcgccagc actgtcgcgc gagctttaaa gtaaaccact tcaccagttt
     9901 cgatgcacag tgcggtacaa ccgaccactg cgccatcctg gtttttcacc agatccagcg
     9961 cataccactc ggagaaaatg gtggtgtggt ttttcaggtt ctgctgataa agcgtgtgca
    10021 acagtgcgtg accggtacgg tcggcagccg ccgcagtacg tgccgcctgc tcgccgccga
    10081 agtttttcga ctgaccgcca aacggacgtt gatagatacg accatcatca agacgcgaga
    10141 acggcaggcc catatgttcc agttccagaa tcgcttccgg cccggtttta cacatatatt
    10201 caatcgcgtc ctggtcaccg atatagtccg acccttttac ggtgtcgtac atatgccatt
    10261 cccagttatc ttcatgggta ttacccagcg caacggtaat accaccttgc gcagaaacgg
    10321 tatgggaacg ggtcgggaag actttagaga gcagcgcaca ggtctggcca ctctgggaaa
    10381 tttgcagcgc cgcgcgcata cctgcgccgc cagcaccaat cacaactgca tcaaattctc
    10441 tgactggcaa tttcatcaca caccccacac cacaacgaat ccataaatca cgtaaaccac
    10501 cagtgcaacg acaatcacca gttgcagcat caggcgcaag gccagcggtt taacgtagtc
    10561 ggtcaacacc tgccacatgc cgatccaggc atggatcaag atagaaaaca gcgccagcag
    10621 ggtgaacact ttggtgaacg cagaggcgaa gaaaccaatc cagacttcat atgtcagctc
    10681 gccactggta gcgaaaaaac cgaccatata aatgatgtag agcgtcagga cgatagcggt
    10741 agcacgaacg aggatgaaat catgtacgcc attgcgtcct aatgcggagg cgttgcttac
    10801 catacgagga ctcctgcgag aagtgaaagc acgacagtaa taacaaagga gattttggcg
    10861 gagcgtttac ccgcttcgaa tgtttcttcc agatagccaa aatccatcat catgtggcga
    10921 atacctacga cgacgtgata tgccagagcg gtaaggatgc cccacatgat aaatttgacg
    10981 aagaagctgc ccataatcgc ggaagcttgc tcgaaacctt caggggaaga gaggctggta
    11041 cccagaagcc acagcaggat gcccactgca acaaaggtga tcacaccgga aacgcgatgg
    11101 agaatggacg ctatcgccgt gacggggaac cggatggtct gtaggtccag attaacaggt
    11161 ctttgttttt tcacatttct tatcatgaat aacgcccaca tgctgttctt attattccct
    11221 ggggactacg ggcacagagg ttaactttct gttaccagga gacgtcggga tttccttcct
    11281 ccggtctgct tgcgggtcag acagcgtcct ttctataact gcgcgtcatg caaaacactg
    11341 cttccagatg cgaaaacgac acgttacaac gctgggtggc tcgggattgc agggtgttcc
    11401 ggagacctgg cggcagtata ggctgttcac aaaatcatta caattaacct acatatagtt
    11461 tgtcgggttt tatcccgaac agtgatctgc gtcacgataa caacatttat ttaattttta
    11521 atcatctaat ttgacaatca ttcaacaaag ttgttacaaa cattaccagg aaaagcatat
    11581 aatgcgtaaa agttatgaag tcggtatttc acctaagatt aacttatgta acagtgtgga
    11641 agtattgacc aattcattcg ggacagttat tagtggtaga caagtttaat aattcggatt
    11701 gctaagtact tgattcgcca tttattcgtc atcaatagat cctttacctg caagcgccca
    11761 gagctctgta cccaggtttt cccctctttc acagagcggc gagccaaata acaaacgggt
    11821 aaagccaggt tgatgtgcga aggcaaattt aagttccggc agtcttacgc aataaggcgc
    11881 taaggagacc ttaaatggct gatacaaaag caaaactcac cctcaacggg gacacagctg
    11941 ttgaactgga tgtgctgaaa ggcacgctgg gtcaagatgt tattgatatc cgtactctcg
    12001 gttcaaaagg tgtgttcacc tttgacccag gcttcacttc aaccgcatcc tgcgaatcta
    12061 aaattacttt tattgatggt gatgaaggta ttttgctgca ccgcggtttc ccgatcgatc
    12121 agctggcgac cgattctaac tacctggaag tttgttacat cctgctgaat ggtgaaaaac
    12181 cgactcagga acagtatgac gaatttaaaa ctacggtcac tcgccatacc atgatccatg
    12241 agcagattac ccgtctgttc catgctttcc gtcgcgactc gcatccaatg gcagtcatgt
    12301 gtggtattac cggcgcgctg gcagcgttct atcacgactc gctggatgtt aacaatcctc
    12361 gtcatcgtga aattgccgcg ttccgcctgc tgtcaaaaat gccgaccatg gccgcgatgt
    12421 gttacaagta ttccattggt cagccatttg tttacccgcg caacgatctc tcctacgccg
    12481 gtaacttcct gaatatgatg ttctccacgc cgtgcgaacc gtatgaagtt aatccgattc
    12541 tggaacgtgc tatggaccgt attctgatcc tgcacgctga ccatgaacag aacgcctcca
    12601 cctccaccgt gcgtaccgct ggctcttcgg gtgcgaaccc gtttgcctgt atcgctgcag
    12661 gtattgcttc actgtgggga cctgcgcacg gtggtgctaa cgaagcggcg ctgaaaatgc
    12721 tggaagaaat cagctccgtt aaacacattc cggaatttgt tcgtcgtgcg aaagacaaaa
    12781 atgattcttt ccgcctgatg ggcttcggtc accgcgtgta caaaaattac gacccacgcg
    12841 ccaccgtaat gcgtgaaacc tgccatgaag ttctgaaaga gctgggtacc aaagatgacc
    12901 tgctggaagt ggctatggag ctggaaaaca tcgcgctgaa cgacccgtac tttatcgaga
    12961 aaaaactgta cccgaacgtc gatttctact ctggtatcat cctgaaagcg atgggtattc
    13021 cgtcttctat gttcaccgtg atcttcgcta tggcacgtac ggttggctgg atcgcccact
    13081 ggagcgaaat gcacagtgac ggtatgaaga ttgcccgtcc gcgtcagctg tatacaggat
    13141 atgaaaaacg cgactttaaa agcgatatca agcgttaatg gttgattgct aagccgttca
    13201 ctttccggac ccgcctttaa tagagcgggt tctttttata tggctgaaca taaaaattgt
    13261 aaaaattaaa ccagatgcaa atacccatta ataaggtaaa aaaatatttc accggaaaca
    13321 ctggtggttg attttggtaa atttaactta ctggatattc gccgccacac aatgtcgaaa
    13381 acattcagta tcaagacaac aaaatcacaa agtgaccagt gtaccgacgg atttaaggtc
    13441 agctcctcgt tttataccga aaaaacgttg gttgaagaag ataaagcatt gttgattggt
    13501 aatggtctga agctacgtat actggatgaa aacgcctcgc cttatacatt caataaatat
    13561 gctgaatacg ccgatttcac cagtgatatg ctgatctacg aaaaaaccta tacggctgaa
    13621 ttatcgtcca cacctggcac tcccatcgcg gcgggtccct tcgatacagt ggtacttttt
    13681 aagattaact ataactgatt taacacgacg cccttttcag ccggaaaggg caacgtattc
    13741 agattattgc tgattttccc cttttccttg attaccgtta ttttttccag acccagcttt
    13801 ctttatgctg tatcgataca ccaaagataa aataatgagg aagcgaagaa tgcctgtatt
    13861 gcagtgggga atgttatgtg ttctgtcact tctcctttct attggcttcc tcgctttaca
    13921 tctcccggcg gcactcctgc ttggcccgat gatcgccggg atcatcttca gtatgcgcgg
    13981 agtcactctg caacttcccc gctccgcttt tctcgctgcc caagccattc ttggctgcat
    14041 gattgcacaa aacctcactg gttcgattct caccacgctg gccgtcaact ggccgatcgt
    14101 gcttgcgatt ttactggtga cgctgctttc cagcgccatc gtgggttggt tattggtgcg
    14161 ctatagctcg ctgccaggaa ataccggggc ctggggttct tctcctggcg gcgcggcggc
    14221 aatggtcgct atggcgcaag attacggcgc agatattcgc ctggtggcgt ttatgcaata
    14281 tctgcgagtg ctgttcgtcg cgggggccgc tgttctggtc acccgcatga tgctgggcga
    14341 taacgctgaa gcggttaacc agcagatcgt ctggttcccg ccagtgagca ttaatctcct
    14401 gcttacgatt ttgctcgcgg tcgttgccgg tacggcggga tgcttgctac gtcttccttc
    14461 cggcacgatg ctcatcccga tgctggcggg cgcagtgctc cagtctggtc agctcatcac
    14521 catcgaactc ccggaatggc tgctggcgat ggcatatatg gcaattggct ggcggattgg
    14581 tcttggtttc gataagcaaa tattactgcg ggcattgcgc ccgctaccgc aaatcctgct
    14641 gtcgattttt gctctgctgg ctatttgtgc gggtatggcg tgggggctga cccggtttat
    14701 gcatattgat tttatgaccg cctacctcgc caccagcccc ggcgggcttg ataccgtggc
    14761 ggtcatcgcc gcagggagca atgccgatat ggcgctcatc atggcgatgc aaaccctgcg
    14821 cctgttcagt attttgctga cggggcctgc catcgcacgg tttatttcaa cctatgcgcc
    14881 gaagcgttcg gcctagtgct ggcagccagg gcaccagtaa aacgggcgcg atgacagcgt
    14941 ggttttctca atgatgccgc cacagcgttc gcacggttcg ccatcacgat gaaaaacctt
    15001 aaagcgaaac agcgccccat gatatttgtt ttcatccacc tggccccgcg tagcgtaaga
    15061 aagtcgcgga atatccagta acgcgtgtgc gagtgcatcc agttgcgccg cattgagatc
    15121 tttcgcttta tgatttccgg tcaacccaac ctgccagagg atctccaccc gcaaataatt
    15181 gccaagtcct gccagaaacg cctgatcgag cagtaatcca gcaaactgac ggttacgaaa
    15241 gcgcggcgac aataatcgtt cctttaccac ctctggcgtc agattcggat ccagcacatc
    15301 gggaccaacg cgttgtaaaa acggatgcgt ggtcagttgt tccggagtca acatctcaat
    15361 atccgaggcg ctataaagca gaatggtttt gtcagccgtt tgcagtttta cccgcaatac
    15421 tcgcgtggtc tgcggctctt cgccggtatc aaccacgcgc cagacgccgt aaagctgatt
    15481 atggctgtag agcgttaagt cgttggaaaa atgagttaac aacgccttac cacgcgtttc
    15541 cacatgggta acgtgttgac cgataagtcg tgattgataa gattttaact gcgggaaggc
    15601 aaaccagaca tcagttagtg gtttgccttt gatcgccgcc tccaggttat ccgctgcacg
    15661 gcggatctcc gggccttcag gcatggtgat atcctttttt cattctttat taagcggggt
    15721 tatgctgcga caacaatccc cttttcagca aatgtagctc gtaatcggcg ggcgaacgcc
    15781 agtgcgtgct cgccatcgcc atgtaggcag accgtttgcg ccgtgaccat tgcccattcg
    15841 ccggtgatac ttttgactct gccgtgttgc accatctcca acgtttgcgc cagcgcctgt
    15901 tcttcgtttt caatcaacgc gcctggctgg cttcgcggca ccagcgaacc gtctgcctga
    15961 taaccacgat cggcaaacac ttcctcgcgc gttacaaggc catatcgttc gcctgcacga
    16021 atcagctcgc ttcctgccag cccgacgaga atcaatgccg gatcgcaagc gtataccgct
    16081 ctggcgatag cgtctgccag ttgtgcctct ttcgccgcct ggttgtataa catgccgtgc
    16141 ggtttgacat gacacattac gccaccttgc gcacgggtaa tcgctgccag cgcgccaatt
    16201 tgatacagcg tctgggcgaa aacggtttct ggcggcagct gcatggcgct gcgaccaaaa
    16261 ttttccctgt cgggaaaact cgggtgagcg ccaatcgcga caccattttt tattgcttca
    16321 cgcacgcaag cctgcatggt ttgcgcatcg cctgcatgaa atccacaggc aatattggca
    16381 gaggaaacca gcgttaatag ctctgcgtcg ctggcgcagc cttcgcccag atcggcgttc
    16441 aggtcaattt tcattgtgca gccgccatgc taattgttcg aaataacgtt gttgatcttg
    16501 ccgtgctttc agtgcctctt ccagtgaaca ctggacaaaa tgaatcggct gaccgagcgg
    16561 aatttgcgcc agatggtaca tatcagcctc aatgatgcag gcaatacgcg ggtaaccacc
    16621 ggtggtttgc gcatcgttca tcaacacaat gggctgcccg ttatgcggca cctgcaccac
    16681 acctggtaac aaaccgtgag ataacagttc gcgatcggtg gtgcgtttta aaatttgccc
    16741 ctgtaagcga tagcccatgc ggttactttg cgagctaagc tgccagggcg aacgccagaa
    16801 tgcatcctgc gaggcgcgat cgaactcatg atattccggc cccggcaagg cgcgaatgcg
    16861 gttgccccac agcagctgtt taacgccctg cgcttccata aaatcacgct tcgctttgcc
    16921 aatcgggagt cggtcaccat ccctcagtaa acggccttcc aatccgccaa tccccacttt
    16981 gagatcggtg ctgcatgatc ccattaccgg cggaacatca ataccacccg cgaccgccag
    17041 ataactgcgc atcccgtgct gcgggcgttt aagcgttaaa cgctggcctg ctctcattgg
    17101 caatcgccag ccggtccaga cggcgttatc atccagccgc gcttcgcaac cggcacccgt
    17161 cagggcaaac cacccatcag tttcgaactc aaccgttaac tgaccgagcg tgatctccag
    17221 cgcgggggcg ttggcgtcat tacccaccag caggttagca atgcgcagag caggcatatc
    17281 cagtgcgccg cagtggctga tacccgactg gcgaaaaccg tgacgaccgc catcctgcac
    17341 agtggtatac atgcccgcac gaataatctt cagcatactc cctccttctg gggtacaaag
    17401 cgcacgctgt ctcccggacg taataagatg ggttcgtcac gctccggatc aaacaggctg
    17461 agtgaggtat gaccaatcaa ctgccagcca cccggcgttg ccagcggata aacaccggtc
    17521 tgcggcccgc cgatcccgac agaacctgct ggaacgtgca agcgcggttc agcgcgccgt
    17581 ggcgtgtgta gttgttccgg caaactcccg agatacggga agcccggttg aaaacctaaa
    17641 aaccagacca cgtattccac ggaggagtgc aattcaacaa cctgtttttc gctcaatcca
    17701 caatgcgccg cgaccaccgc caaatccggt ccggctgcgc caccgtaaac caccggaatt
    17761 tcaataaagc gagactccgg ctccagcgcc tcactctcct cccaccagcg ttgcaaacgc
    17821 tctatggcat ccagcgccag cgactcagga ttacgcaaaa tcactgtgat attgttcatg
    17881 ccaggaatgg cttcaaccac attcggcata ttcaccagac gctgtgccag tcgccagatc
    17941 cgtttctggc tagccagcgt caccggcggg tccagttcca gcactaccgc cgtttcacct
    18001 atcagataac aacgcgctcg ttgcactttc gatccctctg attattcgtt atgcaggatt
    18061 aggaatatca ataaaggtca catcaagatc ggtattttca ttcagccact cgctcaatgc
    18121 gcgaatacca ccacgttcag tggcatggtg acctgcagca taaaaatgca atccctgctc
    18181 gcgggctgaa tgaatggtct gttcagaaac ttcgccagta ataaaagcat ccacgccaaa
    18241 acgcgcggcg ctatcgataa aactttgccc gccgcccgtg caccaggcga cgcgctgaac
    18301 cacctcaggc ccggtatcgc cacaccataa cggcttacgt cccagacgcg cttcaatcca
    18361 ggaggccagt tccagtcccg gcacaggcat ggtcagttcg ccccacggca ccaacggctc
    18421 aatttcgccc atgaccgtga tccccagtaa cgccgccagt tgtgcgttat tacccagctc
    18481 cggatgcgcg tccaggggca gatgccagcc atacaggttg atatcattcg ccagcaacgt
    18541 ttttaaacgg ttacgcttca ttccgcgaat gaccggagat tcgcctttcc agaagtagcc
    18601 atgatgcaca atgaccgcat ccgcgcccag acgcactgcc tcatcgagca aagcctggct
    18661 ggcagtgaca ccggtaacaa ttttttgcac cgtctctttg ccttccacct gcaaaccatt
    18721 cggcgcgtaa tcactaatcg ccgcgctgtt cagtttttcg ttaatcagtt gttccagttc
    18781 ggtgtttttc atcttcgcgc cttccgtatt ctgatagcta caccgttaac atagcgttac
    18841 ctcgcctgtt cgtcgatagc caatcttttg acatattaag aaacaaaccc gacgccaaaa
    18901 gacgcaaatt gtttatatca tcacgcttca gctaatgtat atttatgcaa tcagacagca
    18961 tattgcataa ataatcagat ctgtatcaaa gttttaactt taaggaaaca caagcacgcc
    19021 gcgcaactat tacgtgacgt tgcagtcaat tgtggaatta ttatgaataa acacgcatca
    19081 caaccgcgcg ctatttacta tgtcgttgcg ctgcaaatct gggaatattt tagcttttac
    19141 ggcatgcgtg ccctgctgat cctctatctc accaatcaac taaaatatag cgataatcac
    19201 gcctacgagt tatttagcgc ctactgttcg ctggtatatg tcacgccaat cctcggtggc
    19261 tttttggcgg ataaagttct cggcaatcgc atggcggtga tgctgggggc gttgttgatg
    19321 gcgatcggtc atgtggtgct gggtgccagt gagatccatc cgtcattcct ctatctgtcc
    19381 ctggcgatta tcgtctgcgg ctatggtctg tttaaatcca acgtcagttg cctgctcggc
    19441 gagctgtatg agccaaccga tccacgtcgt gatggcggtt tctcgctgat gtatgcggcg
    19501 ggtaacgtgg ggtctattat cgcacctatc gcctgtggtt tcgcccagga agagtacagt
    19561 tgggcgatgg gctttggcct ggcggcggtg ggtatgatcg cgggtctggt cattttctta
    19621 tgtggcaatc gtcatttcac acatacccgc ggcgttaaca aaaaggtact gcgtgcgaca
    19681 aactttctcc tgcccaactg gggatggcta ctggttctgc tggtggcaac gcctgcgctg
    19741 attaccgtgt tgttctggaa agagtggtcg gtatacgcct tgattgtcgc gacgatcatt
    19801 ggccttgggg ttctggcaaa aatttatcgc aaagcagaaa accaaaaaca gcgtaaggag
    19861 ctggggctga ttgttaccct caccttcttc agcatgttgt tctgggcatt cgcacaacag
    19921 gggggtagct cgattagcct ttatatcgac cgcttcgtta accgcgatat gtttggttat
    19981 accgttccga ctgctatgtt ccagtcgatt aatgccttcg cagttatgct gtgcggtgtg
    20041 ttcctggcgt gggtggttaa agaaagcgtc gcaggtaatc gtaccgtgcg catctgggga
    20101 aaatttgctc ttggccttgg cctgatgagc gccggattct gcattctgac cttaagcgcc
    20161 cgctggtccg caatgtatgg tcactcttct ctgccactga tggtgttagg cctggcggtg
    20221 atgggctttg cggaactgtt tatcgatccg gttgctatgt cgcagattac gcgcattgaa
    20281 atccccggcg tgaccggcgt attaaccggc atttatatgc tgctttccgg cgcgattgcg
    20341 aactatctgg cgggcgtgat tgccgatcag gcatcgcagg cttcgtttga tgcttccggg
    20401 gcgatcaact actccatcaa tgcatatatt gaagtatttg atcaaattac ctggggcgca
    20461 ctggcgtgtg taggactggt actgatgatt tggctgtatc aggcgcttaa gttcagaaac
    20521 cgcgcgctgg cgctggagtc ttaataagcc agcaccatgc ctgataaaac gctcgctgcg
    20581 ttttatcagg ctcctttcct cgcttcttcg tacgctgcca gcgttcgcag ccttgcctct
    20641 ttgtgatcga ctatcggttg cggataatcc aacgtcacac ctgttttctc tgcccacttc
    20701 cacggctcat gcaccgcttt ccccggtaca tcgcgcagct ccggtagcca ctggcggata
    20761 aattcgcctt cgcggtcaaa tttctcgccc tgagttgtcg ggttgaaaat acgaaaatac
    20821 ggcgctgcat cagtacccgt agaagcggcc cactgccagc caccgttatt agctgccaaa
    20881 tcaccatcaa tcagctgcga catgaaatat cgctcgcctt cgcgccagtc gatcaacaaa
    20941 tctttcacca gaaaactggc tgtaatcatc cgtagccggt tatgcatcca gccagtgctg
    21001 ttaagctgac gcatggcagc atcgacaatc gggtatcccg ttttgccttc ctgccaggcc
    21061 tttaaatggg cgggattact ttgccactgt acacgatccg tccaggcaat aaacggacga
    21121 tgtttacaca gcgaaggata atacgtcatc aaatggcggt agaactcgcg ccatatcagc
    21181 tcattaagcc agacgctccc cggtccgcct tccagcacct gcggttgttc cgccaacagg
    21241 cgatgtaaac actggcgagg cgataacccg cccgttgcca ggctggcgga caaacgactg
    21301 gtgccttcca ctgccggaaa atctcgttgt tgctcatact ctccggcacc gttcaggcaa
    21361 aactggcgta attgcgcaat cgccgctttt tcttccaccg ggaaatgcgc agtatcgaaa
    21421 gactgacgag gataattcag cgtaatggat ggcgcgggct ctatcgatcc gctactacga
    21481 acttttggtg cagcgacgca ctccggcatc ccttcccgca gccgtttcaa ccatgcgttt
    21541 ttaaaaggcg taaagacttt gtacatctca tgattgccag tcatcaccgc ccccggcggc
    21601 aggatcacgc tgtcatcaaa tccttcacac accacgttac gcagcgtcct ctcagcctgc
    21661 acatcccgcg cccgctcatt cacctcatac tgatagttat aaaaaagatg agtaacgcgg
    21721 tattccgcgc acacctgttt aactatttcg acgctggcgg caaagtcatc cacttcacgg
    21781 aacaataaag gaataccttt ttccgccagc gcgttttgca gcgcattaag ttgtgtattg
    21841 ataagttcag cctgacgcgg cgacacatta tgcgccgccc actggcgtgg tgtagcgata
    21901 tacaacgcca gcacgcgcgc agacgaattg cggcaggcgg cagccagcgc gagattatcg
    21961 tgctgacgta aatcctggcg aaaccagacc agatgggtag tcataaaact cctgcatggc
    22021 gcaagttaat cacacgcggc caaagttcga aataacgctg ccctgaaagc caggcgtcag
    22081 gataaagcgc catatagtgt ttgatacgca tcagcggcgc aagaagcggt tgctcgccac
    22141 ggcgataact gtcgataagc gccgccagct gctggcgttc tgtggaatca atatgtgggc
    22201 gaaaatatcc ctgaacgtgc atcaaaacgt tagtctgatc acgccggttg gcagggtgag
    22261 aaagcaaaac aattacgcgt tggcggtact ggttgtaata gtcatccaga ctttgccacc
    22321 ggtgaatatc agccacaaac gggccaagtt tgcggtactc cggctgagaa tgtgccagaa
    22381 agacgagttt atagcggctg tgataatcga gaagcgcccc gcgcgttagt cctcgatctt
    22441 tgagattgtg taactcatgc aaagcaaaaa tgcggataat ggtgctgtca tcaaaaagtt
    22501 gttgattcat tactgctcct gaacggttta caggagtaag tgtagacgcc gaaaaaagaa
    22561 aaccgccagc gtttccaccg gcggttttct ttaaacgtca tgtttggcaa tcagtaaaaa
    22621 tcaccagttg ccttttcggc ctgatccatc cacaccggtt tatcgctggt tttcgaccag
    22681 acgcggtgca ggtagctgta aaagcgggcg cggtctttcc agaaaagcat gaaaggcagc
    22741 gccagaacgc ccgctgcaac cgcacaagta cggcgtaaaa agataagcca tgcaggatat
    22801 tctttgtaga gttccatact tttctccccc ttttgtatct acccggtgaa tagcaccgga
    22861 aaaatgaatt cgtttatctg atgaaaatag taccgctgtt tgtgtaattt tactactcat
    22921 ccgaccactt atttttgctt attgatggtt tatttacatt caccgtgtaa ttaagttaca
    22981 taaaagttaa attaatacta aacattagtt aaatcatggc ttttgccatt tttatacttt
    23041 ttttacacca cgcccgcaga tttttgcgaa atctttgcag ccagaactct acccttccgg
    23101 tatcactttt aggccactgg aggtgcactg tgagtgcagg cgtgataacc ggcgtgttgc
    23161 tggtgttttt attactgggt tatctggttt atgccctgat caatgcggag gcgttctgat
    23221 ggctgcgcaa gggttcttac tgattgccac gtttttactg gtattaatgg tgctggcgcg
    23281 tcccttaggt agcgggctgg cgcggctgat taatgatata cctcttcctg gtacgacggg
    23341 cgttgaacgc gtactcttta gcgcgcttgg cgtctctgac cgcgagatga actggaagca
    23401 gtatctctca gccattcttg gcctgaatat actggggctg gcggtgctgt tttttatgtt
    23461 gctcggtcag cactatctgc cgcttaatcc acagcagttg ccggggctgt cgtgggatct
    23521 ggcgctcaat accgccgtca gctttgtcac taacaccaac tggcaatctt atagcggtga
    23581 aaccacgttg agctatttca gccagatggc gggcttaacg gtgcaaaact ttctttctgc
    23641 cgccagcggg attgcggtga tttttgcgct tattcgcgcg tttacccgcc agagcatgaa
    23701 cacgctgggt aatgcgtggg tagacctgtt gcggattacg ttatgggtgt taacccccgt
    23761 agcattgctg atcgcactgt tttttattca acaaggtgcg ctgcaaaact ttttgcctta
    23821 tcaggctgtg acgaccattg aaggagcgca acagctgtta ccaatggggc cagtagcttc
    23881 tcaggaagcg atcaaaatgc tcggaactaa cggcggtggc ttttttaatg ccaactcgtc
    23941 gcatccgttt gaaaacccaa ccgcactgac caacttcgtg caaatgctgg cgattttctt
    24001 gatcccaaca gcgctgtgct ttgcctttgg tgaagtggcg ggcgatcgtc gccaggggcg
    24061 catgttgctg tgggcgatgt cggtgatttt tgtcatctgc gtaggcgtag tgatgtgggc
    24121 agaagttcag ggtaatcctc atctgctggc gctcggcgcg gacagcagca tcaatatgga
    24181 aggtaaagag agccgtttcg gcgtgctggt cagtagcctg tttgcggtcg tgactacggc
    24241 ggcttcctgc ggcgcggtga ttgcgatgca tgattcgttt accgctcttg gtggcatggt
    24301 gccgatgtgg ttgatgcaaa ttggtgaagt ggtattcggc ggcgtcggtt ccggccttta
    24361 cggcatgatg ctgtttgtcc tgctggcggt gtttattgcc gggctgatga ttggtcgtac
    24421 accggaatat ctgggtaaaa aaatcgacgt acgcgagatg aaactgactg cactggcgat
    24481 tctggtcacc ccgacgctgg tgctgatggg cgcggcgctg gcaatgatga ccgacgccgg
    24541 acgtagcgcc atgctcaacc ctggcccgca tggttttagc gaagtgctgt acgccgtgtc
    24601 atccgccgct aacaacaacg gcagcgcctt tgccggatta agcgccaact ctccattctg
    24661 gaactgttta ctggcgttgt gcatgtttgt cgggcgcttc ggggtgatta tcccggtgat
    24721 ggcaattgcc ggttcgctgg tgagtaaaaa gagccaaccc gccagctccg gcacattgcc
    24781 aacgcacggc ccgctgtttg ttggcctgtt aatcggcacc gtgttgctgg ttggcgcact
    24841 gacctttatc cctgccctgg cgcttggtcc ggtggcggaa tatctctcct gatgatattg
    24901 agtgagcact gaatatgagt cgtaaacaac tggcgctatt cgaaccaaca cttgtcgttc
    24961 aggcgctgaa agaagcggtg aaaaaattaa acccgcaggc gcaatggcgc aatccggtga
    25021 tgtttatcgt ctggatcggc agtctgctga ccacctgtat tagcatcgcg atggcaagcg
    25081 gtgtgatgcc tggcaatgcg ctgtttagcg cggcaattag cggctggctg tgggtcaccg
    25141 tactgttcgc taatttcgcc gaggcgttgg cagaaggccg cagtaaagcg caggccaaca
    25201 gtctgaaagg ggtgaaaaaa accgcttttg ctcgcaaact gcgcgaaccg aaatatggcg
    25261 ctgcggcgga caaagttcct gctgatcaac tgcgtaaagg tgacatcgtg ctggtagaag
    25321 ctggcgatat tatcccctgc gatggtgaag ttattgaagg gggtgcatcg gtcgatgaaa
    25381 gcgccatcac cggggaatca gcaccggtga tccgtgaatc cggcggcgat tttgcctccg
    25441 tcaccggcgg cacgcgtatt ctttctgact ggctggtaat tgagtgcagc gttaaccccg
    25501 gtgagacatt tctggatcgg atgatcgcga tggtggaagg tgcgcagcga cgtaaaacgc
    25561 cgaacgagat tgccctgacc attctcctga ttgccctgac tatcgtcttt ttactggcaa
    25621 ccgccacgct gtggccgttt tccgcgtggg gcggtaatgc cgtcagcgta acggtactgg
    25681 tggcactgct ggtctgtctg atcccaacca ctattggcgg cctgttgtca gcgattggcg
    25741 tcgccgggat gagtcggatg ctgggcgcga atgttattgc caccagcggg cgagcagttg
    25801 aagcagcggg tgatgttgac gttctgctgc tggataaaac cggcaccatc accctcggta
    25861 accgtcaggc gtcagagttt atccccgcac aaggcgtgga ggaaaaagcg ctggccgacg
    25921 ccgcacaact ggcttcgctg gctgatgaaa cgccggaagg ccgcagtatt gtgatcctcg
    25981 ccaagcagcg ttttaacctg cgcgagcgcg atgtgcagtc gctccatgcc acctttgtac
    26041 cgtttactgc acaaagccgg atgagcggga tcaacatcga caaccgcatg atccgtaaag
    26101 gttctgtcga tgccattcgc cgccatgttg aggctaacgg cggtcacttc cctgccgatg
    26161 ttgatcaaaa agtcgatcag gttgcgcgtc agggagccac gccgctggtg gtggtggaag
    26221 gttctcgtgt gctgggcgtt attgcgctga aagatatcgt caaaggcggt attaaagagc
    26281 gcttcgccca gctgcgtaaa atgggcatta aaacggtgat gattaccggc gataaccgcc
    26341 tgactgccgc cgcgatagct gcggaagcgg gtgtcgatga ttttctcgcc gaagcaacac
    26401 cggaggccaa gctggcactg attcgtcagt atcaggcgga aggccgttta gtggcgatga
    26461 ccggcgatgg caccaacgat gctccggcgc tggcacaggc cgatgttgcg gtggcgatga
    26521 actccggcac ccaggcggcg aaagaagcgg gcaatatggt ggatctcgac tccaacccga
    26581 ccaagttgat cgaggtagtg cacattggca aacagatgct gatgacccgt ggctcgttga
    26641 ccaccttcag cattgccaac gatgtggcga aatacttcgc cattattcca gcggcgttcg
    26701 cggcaacgta tccgcagtta aatgcgctga acatcatgcg cctgcattcg cccgactccg
    26761 ccattctcag tgcggtcatt ttcaacgcct tgattatcgt ctttttgatt cccctggcgt
    26821 taaaaggcgt gagttataaa ccgcttaccg cttctgccat gttgcgccgt aacttatgga
    26881 tttacggtct gggtgggctg ctggtgccgt ttattggtat caaagtcatt gatttactgc
    26941 taaccgtttg cggtctggtg tgaggtttac catgcgtgga ttacgtccgg cattatcgac
    27001 atttctcttt ctgttattga ttactggcgg cgtttacccg ctgctgacca ccgcactggg
    27061 gcaatggtgg tttccctggc aggccaacgg ttcattgatt cgtgaaggtg atacggtgcg
    27121 cggttcggca ttaatcgggc aaaattttac cggcaacggc tattttcatg gtcgcccgtc
    27181 ggcaacagca gaaatgccct ataatccaca ggcttctggc gggagcaatc tggcagtcag
    27241 taacccggag ctggataaac agatagccgc acgcgttgcc gcattacggg ctgctaaccc
    27301 ggatgccagc acgaacgttc cggttgaact ggtgacggcg tcagcaagcg ggctggacaa
    27361 taacatcacc ccgcaagcgg cggcctggca gatcccacgc gtggcgaaag cgcgtaatct
    27421 cagcgttgaa cagctcacgc aactgatcgc aaaatacagc caacaaccgc tggtgaaata
    27481 tatcggccag ccggtcgtca acattgtcga actcaatctg gcactggata agcttgatga
    27541 ataacgaacc cttacgtccc gaccccgatc gtctgctgga acaagctgcc gcgccgcatc
    27601 gggggaagct gaaagtattc ttcggtgcct gcgcaggcgt cgggaagacc tgggcgatgc
    27661 tggcggaagc ccagcgactg cgggcgcaag ggctggatat tgtggctggc gtggtagaaa
    27721 cccacggtcg gaaagatacc gccgcaatgc tgaaaggtct ggctgttctg ccgttaaaac
    27781 gccaggcgta ccgtgggcgg cacatcagcg agtttgatct cgatgccgcc ctcgcccgcc
    27841 gtccggcact gatcttaatg gacgaactgg cgcacagtaa tgcgccaggt tcacgtcatc
    27901 ctaaacgctg gcaggacatt gaggagctgc tggaagctgg cattgatgtt ttcactaccg
    27961 tcaacgttca gcatctggaa agtctgaatg atgtggtcag cggcgtcacc ggaattcagg
    28021 tacgggagac cgtgcccgat cctttttttg atgccgccga cgacgtggtg ctggtggact
    28081 tgcccccgga cgatctgcgc cagcgactga aagaaggcaa agtctatatt gccgggcagg
    28141 cggagcgcgc cattgaacat tttttccgca aaggtaatct gatcgccctg cgcgaactgg
    28201 cactgcgccg tactgccgat cgcgtagatg agcaaatgcg cgcctggcgg gggcatcctg
    28261 gcgaagagaa agtctggcac acacgcgacg cgatcctttt atgcatcggg cataacaccg
    28321 gcagtgagaa actggtccgc gcggcggcgc ggttggcctc gcgcctgggc agtgtctggc
    28381 acgcggtgta tgttgaaacc cctgccctgc atcgcttacc ggagaaaaaa cgtcgggcaa
    28441 ttctcagcgc cttacgtctg gcgcaggagc tgggcgcgga gacggcaaca ctttctgatc
    28501 cagcggaaga aaaagcggta gtgcgttatg cccgtgaaca taatctcggc aagattattc
    28561 tcggtcgccc ggcttcgcgc cgctggtggc gtcgggaaac atttgctgac cggctggcac
    28621 gcatcgcccc cgatctcgat caggtgctgg tcgcgcttga tgaaccaccc gcccgcacga
    28681 ttaacaacgc gccggatagc cgctctttta aagacaagtg gcgggtacag attcagggat
    28741 gcgtggttgc cgccgcgtta tgcgccgtta tcaccttaat tgccatgcag tggctgatgg
    28801 cgtttgatgc cgccaacctg gtgatgctgt atctgcttgg cgtcgtggtg gtggcactat
    28861 tttacggacg ctggccttcg gtggttgcca ccgtcattaa tgtggtgagt ttcgatctct
    28921 tttttatcgc cccacgcggc acgctcgccg tctctgatgt gcaatatctg ctgaccttcg
    28981 cggtgatgtt aaccgtcggg ctggtgatcg ggaaccttac tgctggcgtg cgttatcagg
    29041 cgcgggtagc ccgttaccgc gaacaacgca cacggcactt atatgaaatg tccaaagctc
    29101 tggctgtggg ccgtagttcg caggatatcg ccgccaccag cgaacagttt attgcctcca
    29161 cgtttcatgc ccgtagccag gtgttgctgc ctgatgacaa cggtaaattg cagcctttaa
    29221 cacatccgca aggaatgacg ccgtgggacg atgccatcgc gcagtggagt tttgataaag
    29281 gcctgcctgc gggcgcgggc accgacacgt tacccggtgt accgtaccag attttgccgc
    29341 taaaaagcgg cgagaaaacc tacggactgg tggtggtgga accggggaat ctgcgccagt
    29401 tgatgatccc ggaacagcag cgcctgctgg agacgtttac gctgttagtc gccaatgccc
    29461 ttgagcgact gacactgacc gccagcgaag aacaggcgcg gatggcaagc gaacgtgaac
    29521 agatccgcaa cgccctgctg gcggcgcttt cgcatgattt acgcacgccg cttacggtgc
    29581 tgttcggtca ggcagaaatc ttaacgctcg atctggcaag cgaaggatcg ccccacgccc
    29641 gccaggccag cgagattcgt cagcatgtgc tgaacaccac ccgactggtg aataatctac
    29701 tggatatggc gcgaattcag tccggcggct ttaatttgaa gaaagagtgg ttaacgctgg
    29761 aagaagtggt cggcagcgcg ctgcaaatgc tggaaccggg tttatcgtcg cccatcaatc
    29821 tttctctgcc agagccgctg accttaatcc acgttgacgg gccactcttt gaacgggtgc
    29881 tgattaacct gctggagaac gcggtgaaat atgcgggggc gcaggccgaa attggtatca
    29941 atgcccacgt tgagggcgaa aatctacaac tggatgtctg ggataacggc cccggtcttc
    30001 caccaggcca ggagcagacg atatttgata agtttgctcg cgggaataaa gagtcggcgg
    30061 taccgggtgt agggcttggg ttggcaattt gtcgggcgat agtggatgta cacggcggca
    30121 ccattaccgc attcaaccga ccggaaggtg gtgcctgttt tcgtgttaca cttccccagc
    30181 aaacagcccc tgaacttgaa gaatttcatg aggatatgtg acaaacgttc tgattgttga
    30241 agatgaacag gctattcgtc gctttctgcg cacggcgctg gagggcgacg gtatgcgcgt
    30301 ctttgaggcc gaaacgctgc aacgcggctt gctggaagcg gcaacccgta agccagattt
    30361 gattattctc gatctcggcc tgcccgacgg tgatgggatt gagtttatcc gcgacctgcg
    30421 ccagtggagc gcggtgccgg tgattgtgct ttccgcacgc agcgaagaga gcgataaaat
    30481 cgccgcgctg gatgccggag cggatgatta tctgagtaag ccgtttggca ttggcgaatt
    30541 gcaggcccgt ctgcgcgtcg cattacgccg ccactccgcc accaccacgc ccgatccgct
    30601 ggtgaaattt tctgatgtta ccgtcgattt agccgcccgc gttatccacc gcggcgagga
    30661 agaggtgcat ctcacaccga ttgagttccg cctgctggcc gtgctgctca acaacgccgg
    30721 aaaagtgctc acccagcgcc agttactcaa tcaggtgtgg ggaccaaacg cggtcgaaca
    30781 cagccactat ttgcgtattt atatggggca tctgcgacaa aaactggagc aagatcccgc
    30841 ccgcc
//
LOCUS       DADSYM010000043        30703 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2415
            SAMN08039579-rid11723173.denovo.043, whole genome shotgun sequence.
ACCESSION   DADSYM010000043 DADSYM010000000
VERSION     DADSYM010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039579
            Sequence Read Archive: SRR6362533
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 30703)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30703)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980162.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 03:39:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,484
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 4,347
            CDSs (with protein)               :: 4,347
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 43 of 85
            Pseudo Genes (incomplete)         :: 37 of 85
            Pseudo Genes (internal stop)      :: 19 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30703
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039579-rid11723173.denovo.043"
                     /strain="LGE2415"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8302 N 95.9628 W"
                     /collection_date="2015-08-24"
     gene            complement(52..720)
                     /gene="artM"
                     /locus_tag="J1Y83_001643"
     CDS             complement(52..720)
                     /gene="artM"
                     /locus_tag="J1Y83_001643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter permease ArtM"
                     /protein_id="HBA7629795.1"
                     /translation="MFEYLPELMKGLHTSLTLTVASLIVALILALIFTIILTLKTPVL
                     VWLVRGYITLFTGTPLLVQIFLIYYGPGQFPTLQEYPALWHLLSEPWLCALIALSLNS
                     AAYTTQLFYGAIRAIPEGQWQSCSALGMSKKDTLAILLPYAFKRSLSSYSNEVVLVFK
                     STSLAYTITLMEVMGYSQLLYGRTYDVMVFGAAGIIYLVVNGLLTLMMRLIERKALAF
                     ERRN"
     gene            complement(720..1436)
                     /gene="artQ"
                     /locus_tag="J1Y83_001644"
     CDS             complement(720..1436)
                     /gene="artQ"
                     /locus_tag="J1Y83_001644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter permease ArtQ"
                     /protein_id="HBA7629796.1"
                     /translation="MNEFFPLASAAGMTVGLAVCALIVGLALAMFFAVWESAKWRPVA
                     WAGSALVTILRGLPEILVVLFIYFGSSQLLLTLSDGFTINLGFVQIPVQMDIENFDVS
                     PFLCGVIALSLLYAAYASQTLRGALKAVPVGQWESGQALGLSKSAIFFRLVMPQMWRH
                     ALPGLGNQWLVLLKDTALVSLISVNDLMLQTKSIATRTQEPFTWYIVAAVIYLVITLL
                     SQYILKRIDLRATRFERRPS"
     gene            complement(1443..2174)
                     /gene="artJ"
                     /locus_tag="J1Y83_001645"
     CDS             complement(1443..2174)
                     /gene="artJ"
                     /locus_tag="J1Y83_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter substrate-binding
                     protein"
                     /protein_id="HBA7629797.1"
                     /translation="MKKVLIAALIAGFSLSATAAETIRFATEASYPPFESIDANNQIV
                     GFDVDLAQALCKEIDATCTFSNQAFDSLIPSLKFRRVEAVMAGMDITPEREKQVLFTT
                     PYYDNSALFVGQQGKYTSVDQLKGKKVGVQNGTTHQKFIMDKHPEITTVPYDSYQNAK
                     LDLQNGRIDSVFGDTAVVTEWLKDNPKLAAVGDKVTDKDYFGTGLGIAVRQGNTELQQ
                     KLNTALEKVKKDGTYETIYNKWFQK"
     gene            complement(2192..2920)
                     /gene="artP"
                     /locus_tag="J1Y83_001646"
     CDS             complement(2192..2920)
                     /gene="artP"
                     /locus_tag="J1Y83_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter ATP-binding protein
                     ArtP"
                     /protein_id="HBA7629798.1"
                     /translation="MSIQLNGINCFYGAHQALFDITLDCPQGETLVLLGPSGAGKSSL
                     LRVLNLLEMPRSGTLNIAGNHFDFTKTPSDKAIRDLRRNVGMVFQQYNLWPHLTVQQN
                     LIEAPCRVLGLSKDQALARAEKLLERLRLKPYSDRYPLHLSGGQQQRVAIARALMMEP
                     QVLLFDEPTAALDPEITAQIVSIIRELAETNITQVIVTHEVEVARKTASRVVYMENGH
                     IVEQGDASCFTEPQTEAFKNYLSH"
     gene            complement(3138..3653)
                     /locus_tag="J1Y83_001647"
     CDS             complement(3138..3653)
                     /locus_tag="J1Y83_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HBA7629799.1"
                     /translation="MRYSKLTMLIPCALLLSACTTVTPAYKDNGPRTGSCVQGGPDSV
                     AQQFYDYRIQHRGNDITALRPYLSDKLATLLSDASRDNSHRELLSSDPFSSRTTLPDS
                     AHVASASTIPNRDARNIPLRVDLKQGDQGWQDEVLMIQEGQCWVIDDVRYLGGSVHAT
                     AGTLRQSIENR"
     gene            3779..4102
                     /locus_tag="J1Y83_001648"
     CDS             3779..4102
                     /locus_tag="J1Y83_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HBA7629800.1"
                     /translation="MQFSTTPTLEGQTIVEYCGVVTGEAILGANIFRDFFAGIRDIVG
                     GRSGAYEKELRKAREIAFEELGSQARALGADAVVGIDIDYETVGQNGSMLMVSVSGTA
                     VKTRR"
     gene            4099..4929
                     /locus_tag="J1Y83_001649"
     CDS             4099..4929
                     /locus_tag="J1Y83_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HBA7629801.1"
                     /translation="MRRVFWLVAAALLLAGCTGEKGIVEKEGYQLDTRRQAQAAYPRI
                     KVLVIHYTADDFDSSLATLTDKQVSSHYLVPAVPPRYNGKPRIWQLVPEQELAWHAGI
                     SAWRGATRLNDTSIGIELENRGWQKSAGVKYFAPFEPAQIQALIPLAKDIIARYHIKP
                     ENVVAHADIAPQRKDDPGPLFPWQQLAQQGIGAWPDAQRVNFYLAGRAPHTPVETASL
                     LELLARYGYDVKPDMTPREQRRVIMAFQMHFRPTLYNGEADAETQAIAEALLEKYGQD
                     "
     gene            complement(4926..5939)
                     /locus_tag="J1Y83_001650"
     CDS             complement(4926..5939)
                     /locus_tag="J1Y83_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415389.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HBA7629802.1"
                     /translation="MKVLVTGATSGLGRNAVEFLCQKGISVRTTGRNEAMGKLLEKMG
                     AEFVPADLTELVSSQAKVMLAGIDTLWHCSSFTSPWGTQQAFDLANVRATRRLGEWAV
                     AWGVRNFIHISSPSLYFDYHHHRDIKEDFRPHRFANEFARSKAASEEVINMLSQANPQ
                     TRFTILRPQSLFGPHDKVFIPRLAHMMHHYGSILLPHGGSALVDMTYYENAVHAMWLA
                     SQEACDKLPSGRVYNITNGEHRTLRSIVQKLIDELNIDCRIRSVPYPMLDMIARSMER
                     LGRKSAKEPPLTHYGVSKLNFDFTLDITRAQEELGYQPVITLDEGIEKTAAWLRDHGK
                     LPR"
     gene            complement(6038..7468)
                     /locus_tag="J1Y83_001651"
     CDS             complement(6038..7468)
                     /locus_tag="J1Y83_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415390.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HBA7629803.1"
                     /translation="MPQRILVLGASGYIGQHLVRTLSQQGHQILAAARHVDRLAKLQL
                     ANVSCHKVDLSWPDNLPALLQDIDTVYFLVHSMGEGGDFIAQERQVALNVRDALREVP
                     VKQLIFLSSLQAPPHEQSDHLRARQATADILREAGVPVTELRAGIIVGAGSAAFEVMR
                     DMVYNLPVLTPPRWVRSRTTPIALENLLHYLVALLDHPASEHRIFEAAGPEVLSYQQQ
                     FEHFMAVSGKRRWLIPIPFPTRWISVWFLNVITSVPPTTAKALIQGLKHDLLADDTAL
                     RALIPQRLIAFDDAVRRTLKEEEKLVNSSDWGYDAQAFARWRPEYGYFAKQAGFTVKT
                     SASLAALWQVVNQIGGKERYFFGNILWQTRALMDRAIGHKLAKGRPEREYLQTGDAVD
                     SWKVIVVEPQKQLTLLFGMKAPGLGRLCFTLEDKGDYRTIDVRAFWHPHGMPGLFYWL
                     LMIPAHLFIFRGMAKQIARLAEQSTD"
     gene            complement(7479..8480)
                     /gene="ltaE"
                     /locus_tag="J1Y83_001652"
     CDS             complement(7479..8480)
                     /gene="ltaE"
                     /locus_tag="J1Y83_001652"
                     /EC_number="4.1.2.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low-specificity L-threonine aldolase"
                     /protein_id="HBA7629804.1"
                     /translation="MIDLRSDTVTRPSRAMLEAMMAAPVGDDVYGDDPTVNALQDYAA
                     ELSGKEAAIFLPTGTQANLVALLSHCERGEEYIVGQAAHNYLFEAGGAAVLGSIQPQP
                     IDAAADGTLPLDKVAMKIKPDDIHFARTKLLSLENTHNGKVLPREYLKEVWEFTRKRN
                     LALHVDGARIFNAVVAYGCELKEITQYCDSFTICLSKGLGTPVGSLLVGNRDYIKRAI
                     RWRKMTGGGMRQSGILAAAGMYALKNNVARLQEDHDNAAWMAEQLREAGADVMRQDTN
                     MLFVRVGEENAAALGEYMKARNVLINASPIVRLVTHLDVSREQLAEVAAHWHAFLAR"
     gene            complement(8517..10235)
                     /gene="poxB"
                     /locus_tag="J1Y83_001653"
     CDS             complement(8517..10235)
                     /gene="poxB"
                     /locus_tag="J1Y83_001653"
                     /EC_number="1.2.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001532507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone-dependent pyruvate dehydrogenase"
                     /protein_id="HBA7629805.1"
                     /translation="MKQTVAAYIAKTLESAGVKRIWGVTGDSLNGLSDSLNRMGTIEW
                     MSTRHEEVAAFAAGAEAQLSGELAVCAGSCGPGNLHLINGLFDCHRNHVPVLAIAAHI
                     PSSEIGSGYFQETHPQELFRECSHYCELVSSPEQIPQVLAIAMRKAVLNRGVSVVVLP
                     GDVALKPAPEGATTHWYHAPQPVVTPEEEELRKLAQLLRYSSNIALMCGSGCAGAHKE
                     LVEFAGKIKAPIVHALRGKEHVEYDNPYDVGMTGLIGFSSGFHTMMNADTLVLLGTQF
                     PYRAFYPTDAKIIQIDINPASIGAHSKVDMALVGDIKSTLRALLPLVEEKTERNFLDK
                     ALEDYRDARKGLDDLAKPSEKAIHPQYLAQQISHFAADDAIFTCDVGTPTVWAARYLK
                     MNGKRRLLGSFNHGSMANAMPQALGAQATEPERQVVAMCGDGGFSMLMGDFLSVVQMK
                     LPVKIIVFNNSVLGFVAMEMKAGGYLTDGTELHDTNFARIAEACGITGIRVEKASEID
                     EALQRAFSIDGPVLVDVVVAKEELAIPPQIKLEQAKGFSLYMLRAIISGRGDEVIELA
                     KTNWLR"
     gene            complement(10368..11336)
                     /gene="hcr"
                     /locus_tag="J1Y83_001654"
     CDS             complement(10368..11336)
                     /gene="hcr"
                     /locus_tag="J1Y83_001654"
                     /EC_number="1.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001522746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH oxidoreductase"
                     /protein_id="HBA7629806.1"
                     /translation="MTMPTNQCPWRMQVHHITQETPDVWTISLICHDYYPYRAGQYAL
                     VSVRNSAETLRAYTISSTPGVSEYITLTVRRIDDGVGSRWLTRDVKRGDYLWLSDAMG
                     EFTCDDKAEDKFLLLAAGCGVTPIMSMRRWLAKNRPQADVQVIYNVRTPQDVIFADEW
                     RNYPVTLVAENNVTEGFIAGRLTRELLAGVPDLASRTVMTCGPAPYMDWVEQEVKALG
                     VTRFFKEKFFTPVAEAATSGLKFTKLQPAREFYAPVGTTLLEALESNNVPVVAACRAG
                     VCGCCKTKVISGEYTVSSTMTLTDAEIAEGYVLACSCHPQGDLVLA"
     gene            complement(11348..13000)
                     /gene="hcp"
                     /locus_tag="J1Y83_001655"
                     /gene_synonym="priS"
     CDS             complement(11348..13000)
                     /gene="hcp"
                     /locus_tag="J1Y83_001655"
                     /gene_synonym="priS"
                     /EC_number="1.7.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308986.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxylamine reductase"
                     /protein_id="HBA7629807.1"
                     /translation="MFCVQCEQTIRTPAGNGCSYAQGMCGKTAETSDLQDLLIAALQG
                     LSAWAVKAREYGIINHDVDSFAPRAFFSTLTNVNFDSPRIVGYAREAIALREALKAQC
                     LAVDANARVDNPMADLQLMSDDLGELQRQAAEFTPNKDKAAIGENILGLRLLCLYGLK
                     GAAAYMEHAHVLGQYDNDIYAQYHKIMAWLGTWPADMNALLECSMEIGQMNFKVMSIL
                     DAGETGKYGHPTPTQVNVKATAGKCILISGHDLKDLYNLLEQTEGTGVNVYTHGEMLP
                     AHGYPELRKFKHLVGNYGSGWQNQQVEFARFPGPIVMTSNCIIDPTVGAYDDRIWTRS
                     IVGWPGVRHLDGEDFSAVIAQAQQMAGFPYSEIPHLITVGFGRQTLLGAADTLIDLVS
                     REKLRHIFLLGGCDGARGERHYFTDFATSVPDDCLILTLACGKYRFNKLEFGDIEGLP
                     RLVDAGQCNDAYSAIILAVTLAEKLGCGVNDLPLSLVLSWFEQKAIVILLTLLSLGVK
                     NIVTGPTAPGFLTPDLLAVLNEKFGLRSITTVEEDMKQLLSA"
     gene            complement(13144..14043)
                     /gene="lysO"
                     /locus_tag="J1Y83_001656"
     CDS             complement(13144..14043)
                     /gene="lysO"
                     /locus_tag="J1Y83_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lysine exporter LysO"
                     /protein_id="HBA7629808.1"
                     /translation="MFSGLLIILVPLIVGYLIPLRQKAALRVINQLLSWMVYLILFFM
                     GISLAFLDNLASNLLAILHYSAVSITVILLCNIAALMWLERGLPWRNHHQQEKLPSRI
                     AMALESLKLCGVVVIGFAIGLSGLAFLQHATEASEYTLILLLFLVGIQLRNNGMTLKQ
                     IVLNRRGMIVAVVVVASSLIGGLINAFILDLPINTALAMASGFGWYSLSGILLTESFG
                     PVIGSAAFFNDLARELIAIMLIPGLIRRSRSTALGLCGATSMDFTLPVLQRTGGLDMV
                     PAAIVHGFILSLLVPILIAFFSA"
     gene            complement(14365..15060)
                     /gene="aqpZ"
                     /locus_tag="J1Y83_001657"
     CDS             complement(14365..15060)
                     /gene="aqpZ"
                     /locus_tag="J1Y83_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin Z"
                     /protein_id="HBA7629809.1"
                     /translation="MFRKLAAECFGTFWLVFGGCGSAVLAAGFPELGIGFAGVALAFG
                     LTVLTMAFAVGHISGGHFNPAVTIGLWAGGRFPAKEVVGYVIAQVVGGIVAAALLYLI
                     ASGKTGFDAAASGFASNGYGEHSPGGYSMLSALVVELVLSAGFLLVIHGATDKFAPAG
                     FAPIAIGLALTLIHLISIPVTNTSVNPARSTAVAIFQGGWALEQLWFFWVVPIVGGII
                     GGLIYRTLLEKRD"
     gene            15486..17144
                     /locus_tag="J1Y83_001658"
     CDS             15486..17144
                     /locus_tag="J1Y83_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent endonuclease"
                     /protein_id="HBA7629810.1"
                     /translation="MILERVEIVGFRGINRLSLMLEQNNVLIGENAWGKSSLLDALTL
                     LLSPESDLYHFERDDFWFPPGDINGREHHLHIILTFRESLPGRHRVRRYRPLEACWTP
                     CTDGYHRIFYRLEGESAEDGSVMTLRSFLDKDGHPIDVEDINDQARHLVRLMPVLRLR
                     DARFMRRIRNGTVPNVPNVEVTARQLDFLARELSSHPQNLSDGQIRQGLSAMVQLLEH
                     YFSEQGAGQARYRLMRRRASNEQRSWRYLDIINRMIDRPGGRSYRVILLGLFATLLQA
                     KGTLRLDKDARPLLLIEDPETRLHPIMLSVAWHLLNLLPLQRIATTNSGELLSLTPVE
                     HVCRLVRESSRVAAWRLGPSGLSTEDSRRISFHIRFNRPSSLFARCWLLVEGETETWV
                     INELARQCGHHFDAEGIKVIEFAQSGLKPLVKFARRMGIEWHVLVDGDEAGKKYAATV
                     RSLLNNDREAEREHLTALPALDMEHFMYRQGFSDVFHRVAQIPENIPMNLRKVISKAI
                     HRSSKPDLAIEVAMEAGRRGVDSVPTLLKKMFSRVLWLARGRAD"
     gene            complement(17141..18097)
                     /locus_tag="J1Y83_001659"
     CDS             complement(17141..18097)
                     /locus_tag="J1Y83_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001351020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF535 domain-containing protein"
                     /protein_id="HBA7629811.1"
                     /translation="MNMSQLTERTFTSSESLSSLSLFLSLARGQCRPGKFWHRRSFRQ
                     KFLLRSLIMPRLSVEWMNELSHWPNLNVLLTRQPRLPVRLHRPYLAANLSRKQLLEAL
                     RYHYALLRGCMSAEEFSLYLNTPGLQLAKLEGKNGEQFTLELTMMISMDKEGDSTILF
                     RNSEGIPLAELTFTLCEYQGKRTMFIGGLQGAKWEIPHQEIQNATKACHGLFPKRLVM
                     EAACLFAQRLQVEQIIAVSNETHIYRSLRYRDKEGKIHADYNAFWESVGGVCDAERHY
                     RLPAQIARKEIAEIASKKRAEYRRRYEMLDAIQPQMATMFCS"
     gene            18248..19363
                     /gene="macA"
                     /locus_tag="J1Y83_001660"
     CDS             18248..19363
                     /gene="macA"
                     /locus_tag="J1Y83_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308991.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide transporter subunit MacA"
                     /protein_id="HBA7629812.1"
                     /translation="MKKRKTVKKRYVIAMVIVIAGLITLWRILNAPVPTYQTLIVRPG
                     DLQQSVLATGKLDALRKVDVGAQVSGQLKTLSVAIGDKVKKDQLLGVIDPEQAENQIK
                     EVEATLMELRAQRQQAEAELKLARVTYSRQQRLAQTQAVSQQDLDNAATEMAVKQAQI
                     GTIDAQIKRNQASLDTAKTNLDYTRIVAPMAGEVTQITTLQGQTVIAAQQAPNILTLA
                     DMSTMLVKAQVSEADVIHLKPGQKAWFTVLGDPLTRYEGQIKDVLPTPEKINDAIFYY
                     ARFEVPNPNGLLRLDMTAQVHIQLTDVKNVLTIPLSALGDPVGDNRYKVKLLRNGETR
                     EREVTIGARNDTDVEIVKGLEAGDEVVIGEAKPGAAQ"
     gene            19360..21306
                     /gene="macB"
                     /locus_tag="J1Y83_001661"
     CDS             19360..21306
                     /gene="macB"
                     /locus_tag="J1Y83_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide ABC transporter ATP-binding
                     protein/permease MacB"
                     /protein_id="HBA7629813.1"
                     /translation="MTPLLELKDIRRSYPAGDEQVEVLKGISLDIYAGEMVAIVGASG
                     SGKSTLMNILGCLDKATSGTYRVAGKDVATLDADALAQLRREHFGFIFQRYHLLSHLT
                     AEQNVEVPAVYAGLERKQRLLRAQELLQRLGLEDRTEYYPAQLSGGQQQRVSIARALM
                     NGGQVILADEPTGALDSHSGEEVMAILHQLRDRGHTVIIVTHDPQVAAQAERVIEIRD
                     GEIVRNPPAVEKVNATGGTEPVVNTASGWRQFVSGFNEALTMAWRALAANKMRTLLTM
                     LGIIIGIASVVSIVVVGDAAKQMVLADIRSIGTNTIDVYPGKDFGDDDPQYQQALKYD
                     DLIAIQKQPWVASATPAVSQNLRLRYNNVDVAASANGVSGDYFNVYGMTFSEGNTFNQ
                     EQLNGRAQVVVLDSNTRRQLFPHKADVVGEVILVGNMPARVIGVAEEKQSMFGSSKVL
                     RVWLPYSTMSGRVMGQSWLNSITVRVKEGFDSAEAEQQLTRLLSLRHGKKDFFTWNMD
                     GVLKTVEKTTRTLQLFLTLVAVISLVVGGIGVMNIMLVSVTERTREIGIRMAVGARAS
                     DVLQQFLIEAVLVCLVGGALGITLSLLIAFTLQLFLPGWEIGFSPLALLLAFLCSTVT
                     GILFGWLPARNAARLDPVDALARE"
     gene            complement(21379..21603)
                     /gene="cspD"
                     /locus_tag="J1Y83_001662"
     CDS             complement(21379..21603)
                     /gene="cspD"
                     /locus_tag="J1Y83_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock-like protein CspD"
                     /protein_id="HBA7629814.1"
                     /translation="MEKGTVKWFNNAKGFGFICPEGGGEDIFAHYSTIQMDGYRTLKA
                     GQSVQFDVHQGPKGNHASVIVPVEVEAAVA"
     gene            21926..22246
                     /gene="clpS"
                     /locus_tag="J1Y83_001663"
     CDS             21926..22246
                     /gene="clpS"
                     /locus_tag="J1Y83_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adapter ClpS"
                     /protein_id="HBA7629815.1"
                     /translation="MGKTNDWLDFDQLAEEKVRDALKPPSMYKVILVNDDYTPMEFVI
                     DVLQKFFSYDVERATQLMLAVHYQGKAICGVFTAEVAETKVAMVNKYARENEHPLLCT
                     LEKA"
     gene            22277..24553
                     /gene="clpA"
                     /locus_tag="J1Y83_001664"
     CDS             22277..24553
                     /gene="clpA"
                     /locus_tag="J1Y83_001664"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpA"
                     /protein_id="HBA7629816.1"
                     /translation="MLNQELELSLNMAFARAREHRHEFMTVEHLLLALLSNPSAREAL
                     EACSVDLVALRQELEAFIEQTTPVLPASEEERDTQPTLSFQRVLQRAVFHVQSSGRNE
                     VTGANVLVAIFSEQESQAAYLLRKHEVSRLDVVNFISHGTRKDEPTQSSDPGSQPNSE
                     EQAGGEERMENFTTNLNQLARVGGIDPLIGREKELERAIQVLCRRRKNNPLLVGESGV
                     GKTAIAEGLAWRIVQGDVPEVMADCTIYSLDIGSLLAGTKYRGDFEKRFKALLKQLEQ
                     DTNSILFIDEIHTIIGAGAASGGQVDAANLIKPLLSSGKIRVIGSTTYQEFSNIFEKD
                     RALARRFQKIDITEPSIEETVQIINGLKPKYEAHHDVRYTAKAVRAAVELAVKYINDR
                     HLPDKAIDVIDEAGARARLMPVSKRKKTVNVADIESVVARIARIPEKSVSQSDRDTLK
                     NLGDRLKMLVFGQDKAIEALTEAIKMARAGLGHEHKPVGSFLFAGPTGVGKTEVTVQL
                     SKALGIELLRFDMSEYMERHTVSRLIGAPPGYVGFDQGGLLTDAVIKHPHAVLLLDEI
                     EKAHPDVFNILLQVMDNGTLTDNNGRKADFRNVVLVMTTNAGVRETERKSIGLIHQDN
                     STDAMEEIKKIFTPEFRNRLDNIIWFDHLSTDVIHQVVDKFIVELQVQLDQKGVSLEV
                     SQEARNWLAEKGYDRAMGARPMARVIQDNLKKPLANELLFGSLVDGGQVTVALDKEKN
                     ELTYGFQSAQKHKAEAAH"
     gene            complement(24896..24983)
                     /locus_tag="J1Y83_001665"
     tRNA            complement(24896..24983)
                     /locus_tag="J1Y83_001665"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(24947..24949),aa:Ser,seq:gga)
     gene            25216..26154
                     /gene="ghrA"
                     /locus_tag="J1Y83_001666"
     CDS             25216..26154
                     /gene="ghrA"
                     /locus_tag="J1Y83_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415551.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate/hydroxypyruvate reductase GhrA"
                     /protein_id="HBA7629817.1"
                     /translation="MDIIFYHPTFDTQWWIEALHKAIPQARVRAWKSGDNESADYALV
                     WHPPVEMLAGRDLKAVFALGAGVDSILSKLQAHPEMLKPSVPLFRLEDTGMGEQMQEY
                     AVSQVLHWFRRFDDYRIQQNSSHWQPLPEYHREDFTIGILGAGVLGSKVAQSLQTWRF
                     PLRCWSRTRKSWPGVQSFAGREELSAFLSQCRVLINLLPNTPETVGIINQQLLEKLPD
                     GAYLLNLARGVHVVEDDLLAALDSGKVKGAMLDVFNREPLPPESPLWQHPRVTITPHV
                     AAITRPAEAVDYISRTIAQLEKGERVCGQVDRARGY"
     gene            26209..26946
                     /locus_tag="J1Y83_001667"
     CDS             26209..26946
                     /locus_tag="J1Y83_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase"
                     /protein_id="HBA7629818.1"
                     /translation="MYPVDLHMHTVASTHAYSTLSDYIAQAKQKGIKLFAITDHGPDM
                     EDAPHHWHFINMRIWPRVVDGVGILRGIEANIKNVDGEIDCSGKMFDSLDLIIAGFHE
                     PVFAPHDKATNTQAMIATIASGNVHIISHPGNPKYEIDVKAVAEAAAKHQVALEINNS
                     SFLHSRKGSEDNCRAIAAAVRDAGGWVALGSDSHTAFTMGEFEECLKILDAVDFPPER
                     ILNVSPRRLLNFLESRGMAPIAEFADL"
     gene            26970..27524
                     /locus_tag="J1Y83_001668"
     CDS             26970..27524
                     /locus_tag="J1Y83_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HBA7629819.1"
                     /translation="MNEFSILCRVLGSLYYRQPQDPLLVPLFTLIREGKLAANWPLEQ
                     DELLTRLQKSCDMTQVSADYNALFIGDECAVPPYRSAWVEGATEAEVRAFLSERGMPL
                     ADTPADHIGTLLLAASWLEDQSTEDESEALETLFSEYLLPWCGAFLGKVEAHATTPFW
                     RTMAPLTRDAISAMWDELEEDSEE"
     gene            27626..28117
                     /locus_tag="J1Y83_001669"
     CDS             27626..28117
                     /locus_tag="J1Y83_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415554.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1097 domain-containing protein"
                     /protein_id="HBA7629820.1"
                     /translation="MNILLSIAITTGILSGIWGWVAVSLGLLSWAGFLGCTAYFACPQ
                     GGLKGLAISAATLLSGVVWAMVIIYGSALAPHLEILGYVITGIVAFLMCIQAKQLLLS
                     FVPGTFIGACATFAGQGDWKQVLPSLALGLVFGYAMKNSGLWLAARSAKTAHREQQIK
                     NKA"
     gene            complement(28181..29014)
                     /gene="csgG"
                     /locus_tag="J1Y83_001670"
     CDS             complement(28181..29014)
                     /gene="csgG"
                     /locus_tag="J1Y83_001670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgG"
                     /protein_id="HBA7629821.1"
                     /translation="MQRLFLLVAVMLLSGCLTAPPKEAARPTLMPRAQSYKDLTHLPA
                     PTGKIFVSVYNIQDETGQFKPYPASNFSTAVPQSATAMLVTALKDSRWFIPLERQGLQ
                     NLLNERKIIRAAQENGTVAINNRIPLQSLTAANIMVEGSIIGYESNVKSGGVGARYFG
                     IGADTQYQLDQIAVNLRVVNVSTGEILSSVNTSKTILSYEVQAGVFRFIDYQRLLEGE
                     VGYTSNEPVMLCLMSAIETGVIFLINDGIDRGLWDLQNKAERQNDILVKYRHMSVPPE
                     S"
     gene            complement(29041..29457)
                     /gene="csgF"
                     /locus_tag="J1Y83_001671"
     CDS             complement(29041..29457)
                     /gene="csgF"
                     /locus_tag="J1Y83_001671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgF"
                     /protein_id="HBA7629822.1"
                     /translation="MRVKHAVVLLMLISPLSWAGTMTFQFRNPNFGGNPNNGAFLLNS
                     AQAQNSYKDPSYNDDFGIETPSALDNFTQAIQSQILGGLLSNINTGKPGRMVTNDYIV
                     DIANRDGQLQLNVTDRKTGQTSTIQVSGLQNNSTDF"
     gene            complement(29482..29871)
                     /gene="csgE"
                     /locus_tag="J1Y83_001672"
     CDS             complement(29482..29871)
                     /gene="csgE"
                     /locus_tag="J1Y83_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgE"
                     /protein_id="HBA7629823.1"
                     /translation="MKRYLRWIVAAEFLFAAGNLHAVEVEVPGLLTDHTVSSIGHDFY
                     RAFSDKWESDYTGNLTINERPSARWGSWITITVNQDVIFQTFLFPLKRDFEKTVVFAL
                     IQTEEALNRRQINQALLSTGDLAHDEF"
     gene            complement(29876..30526)
                     /gene="csgD"
                     /locus_tag="J1Y83_001673"
     CDS             complement(29876..30526)
                     /gene="csgD"
                     /locus_tag="J1Y83_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator CsgD"
                     /protein_id="HBA7629824.1"
                     /translation="MFNEVHSIHGHTLLLITKPSLQATALLQHLKQSLAITGKLHNIQ
                     RSLDDISSGSIILLDMMEADKKLIHYWQDTLSRKNNNIKILLLNTPEDYPYRDIENWP
                     HINGVFYAMEDQERVVNGLQGVLRGECYFTQKLASYLITHSGNYRYNSTESALLTHRE
                     KEILNKLRIGASNNEIARSLFISENTVKTHLYNLFKKIAVKNRTQAVSWANDNLRR"
BASE COUNT         7577 a         7676 c         8094 g         7356 t
ORIGIN      
        1 gtaggccgga taaggcgtcc acgccgcatc cggcatcagc atgcagctac attaattccg
       61 ccgttcgaat gccagtgctt tacgctcgat cagacgcatc atcagcgtca gcaggccgtt
      121 gacgaccagg taaataatcc ccgccgcgcc aaataccatt acatcgtagg tgcgtccgta
      181 caacaactgg ctgtatccca tcacttccat cagcgtaatg gtgtaagcca gagaggtact
      241 tttgaacacc agcaccactt cgttggaata agaagagagc gagcgtttaa acgcgtacgg
      301 cagcaggatc gccagcgtat cttttttgct cattcccagg gcgctacagg actgccattg
      361 accttccggg atcgcgcgaa tcgcaccgta aaacagctgc gtggtgtacg ccgcactgtt
      421 cagcgacaac gcaatcagcg cacataacca cggttctgac aacaaatgcc acagtgccgg
      481 atactcctgc aacgttggga actggcccgg tccgtaataa atcaggaaga tctgcaccag
      541 cagcggcgta ccggtaaaca gcgtgatata gccccgcacc agccacacca gcaccggcgt
      601 tttcagcgtc aggatgatgg taaaaatcaa tgccagaatc agtgccacaa tcagcgaggc
      661 aacggttagc gtcaggctgg tgtgcagccc tttcatcagt tcgggtaaat actcaaacat
      721 tagctgggcc tccgctcaaa acgtgtcgcg cgcaggtcaa tgcgtttgag aatgtactga
      781 ctgagcaggg tgatcaccag gtaaatcacc gccgccacaa tgtaccaggt aaacggttcc
      841 tgtgtacggg tagcgatgct tttggtttgt agcattaaat cattcacact aatcaaactg
      901 accagcgcgg tatctttcag cagcaccagc cactggttac cgagacccgg cagcgcatga
      961 cgccacatct gcggcatcac cagacggaaa aagatagccg atttcgacag ccccagcgcc
     1021 tgaccggatt cccactgacc caccggcacc gcttttaatg caccacgcag cgtttgcgag
     1081 gcataggcgg catacagcag tgacagtgcg atgacaccac aaaggaacgg gctaacgtcg
     1141 aagttctcaa tgtccatctg caccgggatc tgcacgaacc caagattgat agtgaagcca
     1201 tccgaaagcg tcagcagcag ctgcgaggag ccaaaataga taaacagcac caccagaatt
     1261 tctggcaggc cacgcagaat ggttaccagc gctgaacctg cccacgcgac aggacgccat
     1321 ttcgcagact cccataccgc aaagaacatc gccagcgcca gcccgacaat caatgcacaa
     1381 acggcaaggc cgacggtcat cccggcggcg cttgctaaag gaaaaaattc attcatcagg
     1441 aattacttct ggaaccattt gttgtagatg gtttcgtaag tgccatcttt cttcactttt
     1501 tccagcgcag tgttgagttt ctgctgcagt tcagtgttgc cctggcgtac tgcgatgccg
     1561 aggccagtac cgaagtaatc tttatcggtc actttatcgc ccactgccgc cagtttcggg
     1621 ttatctttca gccattcggt taccactgcg gtgtcaccaa atacgctgtc gatacgaccg
     1681 ttttgcagat ccagttttgc gttctggtaa ctgtcatatg gcacggtagt gatttccgga
     1741 tgcttatcca taatgaactt ctggtgtgtc gtcccgttct gaacgccgac ttttttgcct
     1801 ttcagctgat caacactggt gtatttgcct tgctgaccca caaacagggc agagttgtca
     1861 tagtacgggg tggtaaacag cacctgcttt tcacgctccg gagtgatatc catgcccgcc
     1921 atcactgctt ctacgcgacg gaatttcagg cttgggatca ggctgtcaaa cgcctggtta
     1981 gagaaagtgc aggttgcatc aatctcttta cacaatgcct gcgccaggtc gacatcaaaa
     2041 ccaacgatct ggttgtttgc atcaatcgat tcaaacggag gataggaggc ttcggtggca
     2101 aaacgaatgg tttcggctgc tgtggcggaa agactaaaac ctgcaattaa cgcggcaatc
     2161 agaacttttt tcattgttgt tatcccgaat cttagtgaga gagatagttt ttaaatgctt
     2221 cggtttgcgg ctcggtaaag cagctcgcgt cgccttgttc tacgatatga ccgttttcca
     2281 tatacaccac tcggctggcg gttttacgcg ccacttcaac ttcgtgggtg acgatcacct
     2341 gggtaatatt cgtttctgcc agctcacgaa tgatgctgac gatttgtgcc gtaatttcgg
     2401 ggtccagtgc ggcggtcggt tcatcgaaca gcagtacctg cggttccatc atcaacgcac
     2461 gggcaatagc aacacgctgc tgctgacctc cagaaagatg cagcgggtaa cgatcgctat
     2521 aaggtttgag acgcagacgt tccagcagtt tttctgcacg ggccagcgcc tgatctttac
     2581 tcaaccccag tacacgacag ggcgcttcaa tcaggttttg ctgcacggtc agatgcggcc
     2641 acaggttgta ttgctgaaac accatgccaa cgttacgacg caaatcgcga atcgctttgt
     2701 cagagggggt tttggtgaaa tcgaaatggt tgcctgcaat gttgagcgta ccggagcgcg
     2761 gcatctcaag cagattgagt acacgcagca gcgagctttt acccgcgccg ctggggccaa
     2821 gtaacaccag cgtttcgccc tgtgggcaat ccagcgtgat atcgaacagc gcctgatgcg
     2881 cgccgtagaa gcaattaatg ccgtttaatt gaatactcat tgacactcgt atactggcag
     2941 tctgatagct attgaggtcg aagatagtac ctttgacaga ataattatgc aatatttctg
     3001 ctttaaaagt taaaagcaat gcgcattatt caataaacat agcacaaaat aacgggggcg
     3061 gtggtcggcg agcataaatg tcggcattcc tcacgaaatg ccggacaatt tacggggttt
     3121 attggttgat caagacgtta gcgattctcg atggactgac ggagcgtacc cgctgtggca
     3181 tgaacgctac cgcctaagta gcgcacatcg tcgatgaccc aacactggcc ttcctgaatc
     3241 attaacactt catcctgcca gccctggtca ccctgcttga gatccacgcg caatggaatg
     3301 ttacgggcat cacgattagg gatagtagat gcactggcaa cgtgggcgct gtctggcaag
     3361 gtggttcgac tggagaacgg atcgctgctt aacaattcac gatgactatt atcgcggctg
     3421 gcatcgctaa gtagggttgc cagtttgtca ctcaaatagg ggcgcagagc ggtaatgtca
     3481 ttgccgcgat gttgaatgcg gtagtcatag aactgttgag ccacgctatc aggccccccc
     3541 tggacacagg aaccggtgcg ggggccgtta tctttataag ctggcgtgac ggtggtacag
     3601 gcactgagta gcagtgcgca agggataagc atggtcaatt tgctatagcg catgatgatt
     3661 tccttataag cgaacacctt gaaagcgttc tacgataata atgatatcct ttcaataata
     3721 gcgtatcagt ccgataatgc tttgaagatc gaaggcttag caaacaagga gatcgatcat
     3781 gcaattttct acaaccccaa ctctggaagg ccagaccatc gttgaatatt gcggtgtggt
     3841 gactggcgaa gcgattttag gcgcgaatat tttccgtgat ttctttgccg gtatccgcga
     3901 tatcgttggc ggacgctccg gtgcctacga aaaagaactg cgtaaagcgc gggagattgc
     3961 ctttgaggag ctcggctccc aggcgcgagc gctgggggct gatgccgtcg tcggtattga
     4021 tatcgactac gaaacggtcg ggcaaaacgg cagtatgctg atggttagcg tcagcggtac
     4081 ggcggtgaaa acgcgtcgat gagaagagtc ttctggctgg tcgctgccgc tctgttactg
     4141 gcagggtgca caggcgaaaa aggcattgtc gagaaagagg gatatcagct tgatacccga
     4201 cgccaggcgc aggcggcgta tccgcgcatt aaagtgctgg tgatccacta caccgcagat
     4261 gattttgata gctcgctggc gacactgacc gataagcagg tcagctcgca ttatctggtc
     4321 cctgcggtgc caccgcgata caacggtaaa ccgcgcatct ggcaactggt gccggaacag
     4381 gaactggcct ggcatgcggg gattagcgcc tggcgcgggg caacgcgcct taacgacacc
     4441 tctattggca ttgagctgga aaaccgtggc tggcagaaat cagccggagt gaaatatttt
     4501 gccccgtttg aaccggcaca gattcaggcg cttattccac tggcgaaaga tattattgcc
     4561 cgttatcaca tcaagccgga aaacgtagtg gcacatgcgg atatcgcgcc gcagcgcaaa
     4621 gacgatccgg ggccattatt cccctggcag caactggcgc agcaggggat tggtgcatgg
     4681 ccggatgcgc agcgggtgaa cttttacctt gccgggcgcg cgccacacac accggtagag
     4741 accgcgtcat tgctggagct tttggcgcgt tatggttatg acgtaaagcc tgatatgact
     4801 ccgcgcgagc agcggcgcgt gattatggcg ttccagatgc atttccgccc gacgttatat
     4861 aacggcgaag cggatgcaga aactcaggcg attgccgaag cgttgctgga gaaatacggt
     4921 caggattagc gcggcagttt tccgtggtcg cgtagccagg cggcagtttt ctcgatacct
     4981 tcatccaggg tgatgaccgg ctgataacct aactcttcct gcgcacgcgt aatatccagc
     5041 gtaaagtcaa aattcaactt ggagacgccg tagtgggtca gcggcggctc ttttgctgac
     5101 ttgcggccta aacgctccat gctgcgggcg atcatatcca gcatcgggta agggacggaa
     5161 cgaatacgac agtcaatatt caactcgtcg atcagcttct gcacgatgct gcgcagtgtg
     5221 cgatgctcgc cgttggtgat gttgtacaca cgcccggaag gtagcttatc gcaggcttcc
     5281 tggcttgcca gccacatggc gtgcacggca ttttcatagt aggtcatatc gaccagcgca
     5341 ctgccgccgt gcggtaacag aatgctgccg tagtggtgca tcatatgcgc cagacggggg
     5401 ataaagactt tatcgtgcgg tccgaacaga ctttgtgggc gcagaatagt aaagcgcgtt
     5461 tgtggattcg cctgcgaaag catattgatc acttcttcgc tggctgcttt gctacgggca
     5521 aactcgttag cgaagcggtg agggcgaaaa tcttctttaa tatcgcgatg atggtgataa
     5581 tcgaagtaca gggaggggga agagatatga ataaagttac gcacacccca ggcgacagcc
     5641 cattcaccca ggcggcgagt ggcgcgaacg ttagccagat cgaaagcctg ttgtgtcccc
     5701 cagggcgagg taaagctgga gcagtgccac agcgtatcaa tgcccgcgag catcacttta
     5761 gcttgtgatg agaccagctc ggtcagatcc gccggaacaa actctgcgcc cattttttcc
     5821 agcaatttac ccattgcctc gttgcgaccg gtcgttcgca cgctgatgcc tttctggcat
     5881 aaaaactcta ccgcgtttcg acctaagccg ctggtggcgc cggtaaccag taccttcata
     5941 tcaatccact gttgttgaga aaatgacgtg cgcattcttc cgtgatttcc cccatgatgc
     6001 aatgggaaac atgaaagaat aacgcaggtt ttgtcgatta atctgtgctt tgttctgcca
     6061 gtctggcgat ttgttttgcc attccgcgaa aaataaacag atgcgcgggg atcatcaata
     6121 accagtaaaa caaccctggc ataccgtgcg gatgccagaa tgcgcgaaca tcgatagtac
     6181 gatagtcacc tttatcttcc agggtaaaac acagtcgtcc cagccccggc gctttcatgc
     6241 caaataacag cgtaagttgt ttttgcggtt caacgacaat cactttccag ctatccaccg
     6301 catctccggt ctgcaaatat tcacgctccg ggcggccttt cgccagctta tgaccgatcg
     6361 cgcggtccat caacgcccgg gtttgccaca aaatattgcc aaagaaataa cgctctttac
     6421 cgccgatttg attcaccacc tgccataaag ccgcaaggct ggcggacgtt ttaacggtaa
     6481 accccgcctg tttggcgaaa taaccgtact ccggtcgcca gcgggcaaag gcctgagcgt
     6541 cgtatcccca gtcgctggag ttgaccagct tttcttcctc tttcagggtg cgacgtaccg
     6601 cgtcatcgaa agcaatcagc cgttgtggga tgagtgcacg taacgcggtg tcatctgcca
     6661 gcagatcgtg tttcagcccc tgaatcaacg ctttggcggt ggtgggcggt acggaagtaa
     6721 tcacattgag aaaccacacc gaaatccagc gggtggggaa ggggatgggg atcaaccagc
     6781 ggcgcttacc gctcaccgcc ataaaatgtt caaactgttg ctgataactg agcacttctg
     6841 gtccggcggc ttcgaagatg cgatgttcgc tggctggatg gtctaacagc gccaccagat
     6901 agtgcagcaa gttttccagc gcgatgggcg tggtgcgtga acgaacccag cgtggcggcg
     6961 ttaacactgg caggttgtag accatatcgc gcatgacttc gaacgctgct gaacctgcac
     7021 caacgattat tccggcacgc aattcggtca ccgggacacc cgcttcacga agaatatccg
     7081 ccgtagcctg acgggcgcgc agatggtccg actgctcatg tggcggggcc tgcaacgaac
     7141 tgagaaagat taattgctta actgggactt cacgtagcgc atcgcggacg ttgagggcca
     7201 cctggcgctc ctgagcgata aaatcgccgc cttcgcccat gctgtgcacc agaaaataga
     7261 ccgtatcgat atcctgcaac agggccggaa ggttatccgg ccagctgaga tcgactttat
     7321 ggcaactgac atttgccagt tgcagctttg caagcctgtc gacatgacgt gccgccgcca
     7381 ggatctgatg cccttgctgg ctgagcgtgc gcaccagatg ctgaccaatg tagccactgg
     7441 caccgagaac taaaatgcgt tgcggcacgt ctctctcctt aacgcgccag gaacgcatgc
     7501 cagtgggcgg caacttccgc cagttgttca cgcgagacgt caagatgggt caccaggcgg
     7561 acaatcggcg aggcgttaat cagcacgttt ctcgctttca tgtattcgcc taacgcggca
     7621 gcattttctt ccccgacgcg aacaaacagc atattggtgt cctgacgcat cacatccgcg
     7681 cctgcttcac gcaactgctc cgccatccag gcggcgttat cgtggtcttc ctgcaaacgt
     7741 gcgacgttat ttttcagggc atacatcccg gcggcagcca gaatgccgga ctgacgcatc
     7801 ccgccacctg tcattttccg ccagcgaatg gcacgtttaa tgtaatcacg attaccgacg
     7861 agtaatgaac cgactggcgt cccaagacct ttcgacaggc aaatggtgaa cgagtcacaa
     7921 tattgcgtga tctctttcag ttcgcagccg taagccacca cggcattaaa gatgcgcgca
     7981 ccgtcaacat gcagcgccag attgcgcttg cgggtaaatt cccatacctc ttttaagtat
     8041 tcacgcggca gcactttgcc gttgtgggtg ttttccagac tgagtaattt ggtgcgggcg
     8101 aaatggatat cgtcgggttt gattttcatt gccactttat ccagcggtag cgtaccgtcg
     8161 gcagctgcgt cgatgggttg cggctgaata ctgcccagca ctgctgcgcc accggcttca
     8221 aacagatagt tatgcgcggc ctgaccgacg atatactctt cgccgcgttc gcagtgactg
     8281 agcagagcga ccaggttggc ctgagtgccg gtaggcagaa aaatggcggc ttctttacca
     8341 gaaagctctg ctgcatagtc ctgcagagca ttaacggtag ggtcgtctcc gtaaacgtcg
     8401 tccccaaccg gggcggccat catcgcttcg agcatggcgc ggctcggtcg ggtaacggta
     8461 tcactgcgta aatcaatcat ggcatgtcct tattatgacg ggaaatgcca ccctttttac
     8521 cttagccagt tcgttttcgc cagttcgatc acttcatcac cgcgcccgct gatgattgcg
     8581 cgcagcatat acaggctgaa acctttggcc tgttcgagtt tgatctgcgg tggaatggct
     8641 aactcttctt tggcgaccac cacatccacc aacaccggac cgtcgatgga gaaggcgcgt
     8701 tgcagggctt catcgatttc agaggctttt tctacacgaa tacccgtaat tccgcacgct
     8761 tcggcaatgc gggcaaagtt ggtgtcgtgt agttcggtgc cgtcagtcaa atagccacca
     8821 gctttcatct ccatcgccac aaagcccagc acgctgttat taaagacgat aattttcact
     8881 ggcagcttca tctgcactac cgagaggaaa tcgcccatca acatgctaaa accgccatcg
     8941 ccgcacatgg caaccacctg gcgttctggc tcggttgcct gcgcgcccag cgcctgcggc
     9001 atggcgttag ccatcgaacc gtggttaaac gaacctaaca ggcgacgctt gccgttcatt
     9061 tttagataac gtgccgccca caccgttggc gtaccaacgt cacaggtgaa aatagcgtca
     9121 tcggcggcaa aatgactaat ttgctgcgcc agatattgcg ggtgaatggc tttctcgctc
     9181 ggtttagcta aatcgtccag ccctttgcgg gcgtcgcggt aatcttccag cgccttatcc
     9241 agaaaattgc gttcggtttt ttcttccacc agtggcagca atgcgcgcag ggttgatttg
     9301 atatcgccga ccagcgccat atctaccttg ctatgcgcgc cgatgctggc tgggttgata
     9361 tcaatctgaa taattttggc atcggtcggg tagaacgcac ggtagggaaa ctgagtaccg
     9421 agcagcacta acgtatcggc gttcatcatg gtatggaaac ctgacgaaaa gccgattaat
     9481 cccgtcattc caacatcata tggattatcg tattcgacat gttctttacc gcgcagggca
     9541 tgaactatag gcgctttaat tttcccggca aactcaacta actctttatg cgcccccgca
     9601 cagccgctgc cacacatcag ggcgatattg ctggaataac gcagcagttg cgccagtttg
     9661 cgtaactctt cttcttccgg tgttactacc ggctgtggcg catgatacca gtgggtagtt
     9721 gccccttctg gcgcaggttt taacgccacg tcgcccggta acacaacaac ggaaacgcca
     9781 cggttaagca ccgctttgcg catagcaatt gccagcactt gtgggatctg ctctgggctg
     9841 gaaaccagct cgcaatagtg actacattcg cggaatagct cttgtgggtg ggtttcctgg
     9901 aaatagccgc tgccaatttc gctggaggga atatgagcgg caatcgccag taccggaacg
     9961 tgattgcggt ggcaatcgaa caggccgttg attaagtgca ggttgccggg accgcacgat
    10021 ccggcacaga ccgccagttc tccgctaagt tgtgcttcag cgccagcggc aaacgccgcc
    10081 acttcttcat gccgggtgga catccactcg atggtgccca tgcgattaag actgtcacta
    10141 agaccgttca gagagtcgcc cgtgactccc cagatgcgtt tcacgcctgc cgattcgagt
    10201 gttttggcga tataagctgc aaccgtttgt ttcatggttc tccatctcct gaatgtgatg
    10261 acggtaacaa gtttagttca tctgacggag ggggaaggga tgggagaaaa aggaggcact
    10321 aacggttaaa tagcccgatg aaaggaatat catcgggcat caggcgatta tgcgagaacc
    10381 aaatccccct gcggatggca ggagcaggcc agtacgtaac cttcagcgat ttcggcgtcg
    10441 gtcagcgtca ttgtgctgct caccgtatat tcaccggaaa tcactttcgt cttacagcag
    10501 ccgcaaacac ccgcacgaca ggcggcgaca accggaacgt tattgctttc cagcgcctcc
    10561 agtagcgtgg tgccaaccgg agcgtaaaat tctcgtgccg gttgcagttt ggtgaatttc
    10621 agaccgctgg tcgccgcttc cgctactggg gtgaagaatt tctctttaaa gaaacgcgtc
    10681 acgccgagcg ctttcacttc ctgctctacc caatccatat atggcgcagg gccacaggtc
    10741 atcacggtac gtgaggctaa atcagggaca cctgccagca gttcgcgagt gagacgacca
    10801 gcgataaagc cttcggtaac gttattttcc gccaccagcg ttaccggata gttacgccac
    10861 tcatcggcga aaatcacatc ctgcggcgta cgcacgttgt agatcacctg cacatcggcc
    10921 tgtggacggt tcttcgccag ccagcgacgc atcgacataa tcggcgtaac gccgcagcct
    10981 gccgccagca acaggaattt atcttctgct ttatcgtcgc aggtaaattc ccccatcgcg
    11041 tccgaaagcc agagataatc accgcgtttt acatcgcgcg tcagccaccg ggagccgaca
    11101 ccgtcatcaa tccgccgcac ggtcagggtg atgtattcac tcacgcctgg cgtggaggaa
    11161 atggtgtaag cacgcagcgt ttccgctgag tttcgcacgc tgaccagcgc atattgcccg
    11221 gcgcgatatg ggtagtaatc gtggcaaatc agggaaatcg tccacacatc cggcgtttct
    11281 tgcgtaatgt gatgaacctg catccgccac gggcattgat tcgttggcat cgtcatcgac
    11341 aaactcctta cgcgctcaac agttgcttca tgtcttcttc aacagtggtg atagaacgca
    11401 ggccgaattt ctcgttcagc accgccagca ggtctggtgt caggaaacca ggtgcagtcg
    11461 ggccggtgac gatatttttc acacccagag aaagcagcgt cagcagaatg acaatcgctt
    11521 tctgttcaaa ccaggagagc accagcgaca gcggcagatc gttgactccg cagcccagtt
    11581 tctctgccag cgttaccgcc agaataattg ctgagtaagc atcgttacat tgaccagcat
    11641 ctaccagacg cggcagacct tcgatatcgc caaactcaag tttattaaag cgatatttac
    11701 cgcaagcgag ggtcaggatc aggcagtcat ccggcacgct ggtggcgaaa tcggtgaaat
    11761 agtgacgctc gccgcgtgcg ccgtcacagc cgccaagcag gaagatatga cgcagttttt
    11821 cacggctcac cagatcaatc agcgtatcgg ctgcgccaag cagtgtctgg cgaccaaagc
    11881 ccacggtgat caggtgcgga atttcgctgt acgggaagcc cgccatctgt tgcgcctggg
    11941 caatgactgc agagaaatct tcgccatcca gatgacgcac acccggccag ccaacgatgc
    12001 tgcgggtcca gatacgatcg tcataagcgc ctacggttgg gtcgatgatg cagttcgagg
    12061 tcatcacgat ggggcctggg aaacgagcga actccacttg ctgattctgc cagccgctgc
    12121 cgtagttacc gaccagatgc ttgaatttac gcagctccgg gtaaccatgc gcaggcagca
    12181 tttcgccgtg ggtgtagaca ttaacgcccg tgccttcggt ctgttccagc aggttgtaga
    12241 gatctttgag atcgtgaccg gaaatcagaa tgcatttacc cgccgtcgct ttgacgttga
    12301 cctgagttgg cgtcgggtga ccgtatttac cggtttcgcc tgcgtccaga atgctcatca
    12361 ctttgaagtt catctggccg atttccattg aacactcaag aagcgcgttc atatcggcag
    12421 gccaggtccc cagccacgcc atgattttat ggtactgggc ataaatatcg ttgtcgtatt
    12481 gaccgagaac atgcgcgtgt tccatatagg ccgccgcacc tttcaggcca tacaggcaca
    12541 gcagacgcag gccgagaatg ttttcgccaa tcgccgcttt atctttgtta ggggtaaatt
    12601 ctgctgcctg acgttgcagc tcgccgagat cgtcgctcat cagttgcagg tcagccatcg
    12661 ggttatcgac gcgggcgttg gcatctacag ccaggcattg tgctttcagc gcctcgcgca
    12721 gggcaatcgc ttcacgagcg tagccgacaa tacgcggaga atcgaagtta acgttggtca
    12781 gggttgagaa aaaggcacgt ggcgcaaagc tgtctacatc gtggttgatg atgccgtatt
    12841 cacgtgcttt cactgcccag gccgaaagcc cttgcagcgc cgcgatgagt aaatcctgaa
    12901 ggtcagaggt ttccgccgtt ttaccacaca tcccctgcgc gtatgagcag ccgtttcctg
    12961 ccggagtacg gatagtttgt tcacattgca cacaaaacat gatcacacct tttaaagtta
    13021 tatttaatat acatgtttaa ggttaagacg cttaacgcgg ggataaaaga gatttttaat
    13081 gcaactttaa gggagattga tttagcgcaa ttttggcggc agagatctac cgccagagag
    13141 gtattatgca gagaaaaagg cgatgagaat cggcactaac agactaagaa taaaaccgtg
    13201 aacaatcgcc gccgggacca tatccagccc gcctgtacgt tgaagaacgg gcagggtgaa
    13261 atccatcgac gtcgcaccgc ataagcccag tgcagtagag cggctacggc gaatcagccc
    13321 agggattaac ataatagcaa tcagctcacg ggccagatca ttaaaaaacg ccgcgctccc
    13381 gattaccgga ccaaaagatt cggtcaataa aataccggaa agagaatacc agccgaaacc
    13441 ggaggccatt gccagcgcgg tattgatggg aagatcaaga ataaaggcgt taattaaacc
    13501 accaattaat gaactggcaa ccaccaccac ggcgacaatc attccccggc gattaaggac
    13561 aatctgcttt aaggtcatgc cattattgcg caactgaata ccgacgagga aaagtagcag
    13621 aattaaggtg tattcactgg cttcggtcgc gtgttgtaag aaagccagtc cactcagacc
    13681 aatggcaaaa ccaatcacta ctacgccgca cagttttagc gactccagcg ccatcgcaat
    13741 acgcgacggg agtttttctt gctgatggtg gttgcgccat ggcaggccgc gctccagcca
    13801 catcagggcg gcaatattac acagtaaaat aacggtaata ctgacggcag aataatgcag
    13861 aattgccaac aagttactgg cgaggttatc gagaaacgcc agactgatac ccataaaaaa
    13921 gagaataagg taaaccatcc aacttaatag ctgattaata actcttaatg cagctttttg
    13981 gcgaagcgga atgaggtaac ccacaatcag gggaaccaga atgattaaca gcccagaaaa
    14041 catgaaaaac cagtccttgc aaagaagatt cgaaatgcgc gatgacacac tactgaaagc
    14101 ggaaggacga gtaaagttgc aattaaaagg aaatgttatg catcagaggg ggatttttaa
    14161 ttctatatga gtgtttagcg ttgttgcgct cctttcatgt gcaatgccat ataaagccac
    14221 tggcgcaggg agcggccagt cgccgccgcc ccctgccccc cccggccttc ggggaacgcc
    14281 tcggcgattt taacgccacc aacacccgtg cggtttgtaa cgtcgaagat aaaaccacca
    14341 gggcagacaa catgccaatg ctaattaatc gcgtttttcc agtagagtcc ggtaaatcag
    14401 accaccgata ataccgccga caattggcac cacccagaag aaccacagtt gttctaatgc
    14461 ccagccgccc tggaagatag caaccgcagt actgcgcgcc gggttaacgg aagtgttagt
    14521 caccggaata ctgattaagt gaatcagggt taaggccaga ccaatggcaa ttggcgcaaa
    14581 accagcagga gcgaatttgt cagttgcgcc gtggatcacc agcaggaaac ctgcactcaa
    14641 taccagttca actaccagcg cggaaagcat ggaataaccg cctggtgaat gctcgccata
    14701 accgttagaa gcaaaaccgc tggctgccgc gtcaaaaccc gttttaccac tggcaattaa
    14761 atacagcagc gccgccgcaa caataccgcc gacaacctgg gcaattacgt agccaacgac
    14821 ttcttttgcc gggaaacgtc cgccagccca taaaccaata gtgaccgccg ggttaaaatg
    14881 accaccagaa atatgaccaa cagcaaaggc cattgtcaga acggtcagac cgaacgccaa
    14941 cgccacgccg gcaaaaccaa tgcctaattc cgggaagcct gcggccagta cggcactacc
    15001 acagccgcca aaaacaagcc agaaagtacc aaaacattca gctgctaatt ttctgaacat
    15061 atccacctca attaaaaatt gaccctgtga aaaatatggt cgttttatag ggtcgtcgta
    15121 aaaaagtgac gacggaaata atgcgcggct attttaaaaa cgaaggcaaa tcattcgcca
    15181 gataaataaa tccagtaaat ttgatttagg gcaacagcgg gttgccccat atagtcattt
    15241 gtctgattga cagtgtagtg caagcgaaag atttaatcct ttaggcgtaa taaaaaataa
    15301 tttatcatgc taattatttg attttgttgt ttttgcagac ttatcagcaa gagggagtat
    15361 aacgcgatta ttcgctcatt tttcagacat ttgccatgct taaatgtgat gtcatcacgt
    15421 attagcatgg cctttcccgt tatactgcca gcgtaaagga taagtcacat atttctggag
    15481 gggatatgat tcttgagcgc gttgaaattg tgggttttcg cggtatcaac cgtttgtcgt
    15541 tgatgctgga acaaaacaac gtcctgattg gggagaacgc gtggggtaaa tccagcttgc
    15601 tggacgcctt aaccctgctg ctatcgccag aatcagatct ctaccatttt gagcgcgacg
    15661 atttctggtt cccgccggga gatatcaacg ggcgagaaca tcatctgcat attattttga
    15721 ccttccgcga atcgctgcca ggccgacatc gggttcgccg ctatcggccg ctggaagcgt
    15781 gctggacgcc atgcaccgat ggttatcacc gtatttttta tcgtctggaa ggggagagtg
    15841 cggaagacgg cagcgtgatg acactccgca gttttctcga taaagacgga catccgattg
    15901 atgttgagga tattaacgat caggctcgtc atctggtgcg tttaatgccg gtgctgcgct
    15961 tgcgtgatgc ccgttttatg cgccgtattc gtaacggcac ggtgccaaat gtccccaatg
    16021 tggaagtcac cgcgcgccag ctcgatttcc tcgcccgtga gttgtcctca catccgcaaa
    16081 atctctctga tggacagatt cgtcagggac tttccgcaat ggtgcaactg cttgagcatt
    16141 atttttctga gcagggagct ggacaggcgc gatatcgttt aatgcggcgg cgagccagca
    16201 atgagcaacg aagctggcgc tatctggata tcatcaaccg gatgattgac cgacctggag
    16261 ggcgctcgta tcgggttatt ttgctcggcc tgtttgctac tttgttgcag gcaaaaggca
    16321 cattgcgact ggataaagac gcccgtccat tgttgctgat cgaagatcca gaaacccgtt
    16381 tacaccccat tatgctttca gttgcctggc atctgttgaa tcttctgcca ttgcagcgca
    16441 tcgctactac caactcggga gagttgcttt cgttaacgcc ggtagagcat gtttgccgac
    16501 tggtacgtga gtcctctcgc gttgccgcct ggcgtctggg accgagtggc ttgagtaccg
    16561 aagatagccg acgcatatcc tttcacattc gttttaaccg tccgtcatcg ctgtttgctc
    16621 gctgctggct gttggtggaa ggggaaacgg aaacctgggt tatcaacgaa ctggcgcgcc
    16681 agtgtggaca ccatttcgat gccgaaggga tcaaggtcat tgagtttgcc cagtccgggc
    16741 taaagccgct ggttaaattt gcccggcgaa tggggattga atggcatgta ctggtcgatg
    16801 gcgatgaagc agggaagaaa tatgccgcta cggtacgcag cctgttgaat aacgatcggg
    16861 aagccgaacg agaacattta acggcgttac cggcgctgga tatggaacat tttatgtatc
    16921 gccagggatt ttccgatgtg ttccaccgcg tggcgcaaat cccggaaaat ataccgatga
    16981 atctgcgcaa agttatctcg aaagcgatcc atcgctcttc caaacccgat cttgccattg
    17041 aagtggcaat ggaggccgga cgtcgtggtg tggattccgt accgactctg ctgaaaaaaa
    17101 tgttctcacg cgtgctgtgg ctggcgcgcg gtcgggccga ctaactacaa aacatcgtgg
    17161 ccatttgtgg ctgaatagcg tcgagcatct catagcgccg acggtattca gcccgttttt
    17221 tactggcgat ttcggcaatc tcttttcgtg ctatctgtgc tggaaggcgg taatggcgtt
    17281 cagcatcaca tacgccgcca accgattccc agaaagcgtt gtaatcggca tggatcttgc
    17341 cttctttatc gcgataacgc aggctgcggt aaatatgcgt ttcattgctg acggcaataa
    17401 tctgctctac ctgcaaacgt tgggcaaaca gacaggccgc ttccatcacg aggcgtttgg
    17461 gaaatagccc gtggcaggct ttcgtcgcat tctggatttc ctggtgtgga atttcccatt
    17521 ttgcgccttg cagtccgcca ataaacatcg tccttttccc ctgatattca cacagggtaa
    17581 aagtgagctc tgccagagga ataccttcgc tgttgcggaa caggatggtg ctgtcacctt
    17641 ctttatccat tgagatcatc atggtcagct caagcgtgaa ctgctcgccg tttttgcctt
    17701 ccagcttcgc cagttgcagc ccgggggtat tcaaatataa gctgaattct tccgccgaca
    17761 tacacccgcg gagtaacgca taatggtaac gtaacgcctc cagcaattgc ttacggctaa
    17821 gattcgccgc aaggtaaggg cgatgtagac gtacaggcag tcgcggctgg cgcgttaaca
    17881 acacattgag attaggccag tgggaaagtt cgttcatcca ctcaacgctt aaacgcggca
    17941 taatcaacga gcgcagcaaa aatttctggc gaaaactacg gcgatgccag aatttacccg
    18001 gccgacactg tcctcgtgcc agactaagaa aaagtgacag gctgctgaga gattcagatg
    18061 acgtaaaggt ccgttcagtt agctgcgaca tattcatgaa atcaatggtt atacatgacg
    18121 tcgatttaac cattgcgtat cttaaccaaa catcaatagt gtgattatta acgtaaattt
    18181 tagtgttttg ttgatatttc gttgaagtta atgacccgga ttggcatatg gagtattcag
    18241 aatatttatg aaaaagcgga aaaccgtgaa gaagcgttac gttattgcga tggtgatagt
    18301 catcgccggg ctgattacgt tatggagaat tcttaacgca cccgtgccga cttatcagac
    18361 gctgattgtg cgccccggtg atttacagca aagcgtgctg gcgaccggaa agctggacgc
    18421 gctgcgtaag gttgacgtgg gcgcgcaggt cagcggtcag ttgaaaacgc tgtcggtggc
    18481 gattggcgat aaagtaaaaa aagaccagct tttaggggtt attgatcctg aacaggctga
    18541 aaaccagatc aaggaggtgg aagcaacgct gatggagcta cgcgcgcagc ggcagcaggc
    18601 ggaagcggag ctgaaactgg cgcgggtgac gtattcccgt cagcaacgtc ttgcacaaac
    18661 gcaagcggtt tcacagcagg atctcgataa tgccgcgacg gagatggctg tgaaacaggc
    18721 gcaaattggc accattgacg cgcaaatcaa gcgcaatcag gcctctctcg atacggctaa
    18781 aaccaatctc gattacaccc gcatcgttgc cccgatggcc ggggaagtca cgcaaatcac
    18841 cactctgcaa ggccagacgg tgattgccgc acaacaagca ccgaacattc tgacgctggc
    18901 agatatgagc accatgctgg taaaagcgca ggtttctgaa gcggatgtaa tccacctgaa
    18961 gccggggcaa aaagcctggt ttacggtgct tggcgatcca ctgacgcgct acgaggggca
    19021 aatcaaggat gtgctaccga cgccggaaaa gattaacgac gctattttct attacgcccg
    19081 ttttgaagta cccaacccca atggtttact gcggctggat atgaccgcgc aagtgcatat
    19141 tcagctcacc gatgtgaaaa atgtgctgac gatccctctg tcggcgttag gcgatccggt
    19201 tggcgataat cgttataaag tcaaattgtt gcgtaatggt gaaacacgcg agcgtgaagt
    19261 gacgattggc gcacgtaacg ataccgatgt tgagattgtc aaagggctgg aagcgggcga
    19321 tgaagtggtg attggtgagg ccaaaccagg agctgcgcaa tgacaccctt gctcgaatta
    19381 aaggatattc gtcgcagcta tcctgccggt gatgagcagg ttgaggtgct gaagggcatc
    19441 agcctcgata tttatgctgg tgagatggtg gcgattgtcg gcgcttcggg ttccggtaaa
    19501 tcgaccctga tgaatattct cggctgcctg gataaggcca ccagcggcac ctatcgcgta
    19561 gccggtaagg atgttgccac gctggacgcc gatgcgctgg cgcaactgcg ccgcgagcat
    19621 ttcggcttta ttttccagcg ttaccatttg ctttcgcatt taaccgccga gcagaacgtt
    19681 gaagtacccg ccgtctatgc cggtcttgag cggaaacagc ggctgcttcg cgcccaggag
    19741 ttactgcaac gactggggct ggaagaccgt acagagtatt acccggcaca gctttcgggt
    19801 ggccagcaac agcgcgtcag catcgcgcgg gcattgatga acggcggtca ggtaattctt
    19861 gccgatgaac cgaccggcgc actggacagc cattctggcg aagaggtgat ggcgatcctg
    19921 catcagctgc gcgatcgcgg gcatacggtg attatcgtca cccacgatcc gcaggttgct
    19981 gctcaggccg agcgggtgat cgaaattcgc gacggcgaaa ttgtgcgcaa tcctcccgcc
    20041 gttgaaaaag tgaatgctac tggcggaacg gaacctgttg tcaacacggc gtctggctgg
    20101 cggcagtttg tcagcggttt taacgaggcg ctgacgatgg catggcgggc gctggcagcg
    20161 aataaaatgc gtactttact gaccatgctg gggattatta tcggtattgc gtcggtggtt
    20221 tccattgtcg tggtgggcga cgccgccaaa caaatggtgc tggcggatat tcgttctatt
    20281 ggtacgaata ctattgatgt ctatcccggg aaagattttg gcgatgacga tccgcaatat
    20341 cagcaggcgc tgaagtacga cgatttgatc gccattcaaa aacagccgtg ggttgcctct
    20401 gccacgcccg ccgtctcgca aaacctgcgc ctgcgttata acaatgttga tgtggctgcc
    20461 agtgccaatg gcgtgagcgg cgattatttt aatgtctatg gcatgacctt cagtgaaggg
    20521 aacaccttta atcaggagca gctgaacggt cgtgcgcagg tcgtggttct cgacagtaat
    20581 actcgccgcc agcttttccc ccataaagca gatgtggttg gcgaggtgat tctggtcggc
    20641 aatatgcccg ccagagtcat tggtgtggcg gaagaaaaac agtcgatgtt tggtagcagt
    20701 aaagtgctgc gtgtctggct gccatacagc acgatgtccg ggcgagttat ggggcagtcg
    20761 tggctgaact ccattactgt cagggtgaaa gaaggctttg acagcgctga ggcggaacag
    20821 caactcacgc gtttactttc actgcgccac ggtaagaagg atttctttac ctggaacatg
    20881 gatggcgtct tgaaaaccgt tgaaaagacc acacgtactt tacaactgtt tctgacgctg
    20941 gtggcggtga tttcgctggt ggtgggcggt attggtgtaa tgaatattat gctggtgtca
    21001 gtgaccgagc ggacgcggga aattggcatt cgcatggctg taggtgcgcg agcaagcgat
    21061 gttttgcaac agtttttgat cgaagccgta ctggtttgcc tggttggtgg cgcattggga
    21121 ataacactgt cactgttaat tgctttcacc ttgcagcttt tcttacccgg ctgggagatt
    21181 ggcttttcac cgttggcact gctgctggcg tttctctgct cgacggtcac cgggatttta
    21241 tttggctggt tacccgcacg aaatgcggca cgactggatc cagtagatgc tctggcacga
    21301 gagtaatttt tgagataaaa atgccagccg atcgggctgg cattttgcct ttaggatgta
    21361 cacaatgaga cagaagagct atgcgactgc cgcttctact tcgacgggca caataacact
    21421 ggcgtgattg ccttttggcc cctggtggac atcaaactga acggattgtc cagcttttag
    21481 cgttctgtaa ccatccatct gaatggtgga ataatgagcg aaaatatctt cgccgccgcc
    21541 ttcagggcag atgaaaccaa accctttggc attgttgaac cacttaacag tacccttttc
    21601 catgcttcga catccttcgc aaatcttata caagtaagat ggaataaacc ggggtcagag
    21661 agggggctgt tcaaaacctc gccaactcta gaaatacaat ttagagaatt agggcgagcc
    21721 gtcaagcatt tgacaggggc cagggggcag gtatgaatca aaaatttgaa gcagttaacg
    21781 ctattgacag gaatgtgaca gatgtcgctg atgctaacga tagatgatag ttatctatca
    21841 tgtggagtag attggtcagg caaataagct cttgtcagcg gcagggcgtt ctgccgataa
    21901 ccgtaaccga agatgataac tgacaatggg taaaacgaac gactggctgg actttgatca
    21961 actggcggaa gaaaaagttc gcgacgcgct aaaaccgcca tctatgtata aagtgatatt
    22021 agtcaatgat gattatactc cgatggagtt tgttattgac gtgttacaaa aattcttttc
    22081 ttatgatgta gaacgtgcaa cgcaattgat gctcgctgtt cactaccagg ggaaggccat
    22141 ttgcggagtc tttaccgccg aggttgccga aaccaaagtg gcgatggtga acaagtacgc
    22201 gagggagaat gagcatccat tgctgtgtac gctagaaaaa gcctgaatgc aggcataaaa
    22261 attgggggag gtgcctatgc tcaatcaaga actggaactc agtttaaata tggctttcgc
    22321 cagagcgcgc gagcaccgtc atgagtttat gaccgtcgag cacttgttac tggcgctgct
    22381 cagtaaccca tctgcccggg aggcgctgga agcgtgttct gtggatttgg ttgcgctccg
    22441 tcaggaactg gaagccttta ttgaacaaac cacacccgtt ctgcctgcca gtgaagagga
    22501 gcgcgacaca cagccgacgc tgagttttca gcgtgtactg caacgtgcgg tcttccatgt
    22561 ccagtcctcc ggtcgcaatg aggttaccgg tgcaaacgtt ctggtcgcta tctttagcga
    22621 acaggagtcg caggcggcat atctgttgcg caaacatgaa gtcagccgtc tcgatgtggt
    22681 gaactttatc tctcatggca cgcgtaaaga cgagccgaca cagtcttctg atcctggcag
    22741 ccagccaaac agcgaagaac aagctggtgg ggaggaacgt atggagaatt tcacgacgaa
    22801 cctgaatcag cttgcgcgag tgggcggaat cgacccactg attggtcgtg agaaggagct
    22861 ggagcgtgct attcaggttc tctgccgtcg ccgtaaaaac aacccgctgc tggtggggga
    22921 gtctggtgtc ggtaaaaccg cgattgcgga ggggcttgca tggcgaattg ttcagggcga
    22981 tgtgccggaa gtgatggctg actgtacgat ttactctctc gatatcggtt ctctgttagc
    23041 gggcacaaaa tatcgcggcg actttgaaaa acgttttaaa gcgttgctca agcagctgga
    23101 gcaggacact aacagcatcc tgtttattga tgagatccac accattatcg gtgcgggtgc
    23161 agcgtctggt ggccaggtcg atgcggctaa cctgattaaa ccgttgctct ccagcggtaa
    23221 aattcgcgta attggttcga caacctatca ggagttcagc aacatttttg agaaagaccg
    23281 tgctctggcg cgtcgcttcc agaaaattga tattactgaa ccgtcaatcg aagaaactgt
    23341 tcaaatcatc aatggcctga aaccgaagta tgaagcgcac cacgacgtgc gttataccgc
    23401 aaaagcggtg cgtgcggcgg tagagctggc ggtgaaatac attaacgatc gtcatctgcc
    23461 ggataaagcg attgatgtta tcgacgaagc gggcgctcgc gcacgtctga tgccggtaag
    23521 caaacgcaag aaaaccgtta atgtggcgga tattgaatcc gtggtggccc gtattgcacg
    23581 cattccagag aagagtgttt ctcagagtga ccgcgatacc ctgaaaaacc tcggcgatcg
    23641 cctgaaaatg ctggtcttcg gtcaggataa agccattgag gcgctgactg aagccattaa
    23701 gatggcgcgt gcaggtttag gtcacgaaca taaaccggtc ggttcgttcc tgtttgccgg
    23761 ccctactggg gtcgggaaaa cagaggtgac ggtacagctt tcgaaagcgt tgggcattga
    23821 gcttctgcgc tttgatatgt ccgagtatat ggaacgccat accgtcagcc gtctgattgg
    23881 tgcgcctccg ggatacgttg gttttgatca gggcggtttg ctgactgatg cggtcatcaa
    23941 gcatccacat gcggtgctgc tgctggacga aatcgagaaa gcgcacccgg acgtgttcaa
    24001 catcctcttg caggtgatgg acaacggtac actgaccgat aacaacggac gcaaagcgga
    24061 cttccgtaac gtggtgctgg tgatgaccac taacgcgggg gtacgtgaaa ccgagcgcaa
    24121 atccattggt cttatccacc aggataacag caccgatgcg atggaagaga tcaagaagat
    24181 ctttacaccg gaattccgta accgtctcga caacattatc tggttcgatc atctgtcaac
    24241 cgacgtgatc catcaggtgg tggataaatt catcgtcgag ttgcaggttc agttggatca
    24301 gaaaggtgtt tctctggaag tgagccagga agcgcgtaac tggctggccg agaaaggtta
    24361 cgaccgggca atgggcgcac gtccgatggc gcgtgtcatc caggacaacc tgaaaaaacc
    24421 gctcgccaac gaactgctgt ttggttcgct ggtggacggc ggtcaggtca ccgtcgcgct
    24481 ggataaagag aaaaatgagc tgacttacgg attccagagt gcacaaaagc acaaggcgga
    24541 agcagcgcat taatctgatt gtctggtagg ttggttaagt ccgtgatctc gtcaggggtt
    24601 acggactttt tatttatggg gggaggaggt tcagaccctt tttttaatga tgatgctaag
    24661 ttattgataa ttagtgctgc gggtaggtaa ggataaaaaa gggtggcagc aggagattga
    24721 gatggttttg ctttattaac aacgggctaa acatgtagta tttgagttca ctgccgtaca
    24781 ggcagcttag aaattgccgc ggatcctgtc tgccaataat gacaagttca ctgccgtaca
    24841 ggcagataaa atgcgaaaaa aaacccgtac tttcgtacga gctcttcttt aaatatggcg
    24901 gtgagggggg gattcgaacc cccgatacgt tgccgtatac acactttcca ggcgtgctcc
    24961 ttcagccact cggacacctc accaaattgt cgttcctgtc ttgctggaac gggcgctaat
    25021 ttagggaaat catgacctga ggtcaacaaa ctttttaaaa aaatcgctcg attattcaaa
    25081 cttcaatcaa tatgcagttt taataagcga aatttgcttt ttttgccacc gaccacggat
    25141 ttgttatgct ggtggtcttt gtagatcata acgataagtg cgaataaatt tcgccaacac
    25201 tcttcgggag tcagtatgga tatcatcttt tatcacccaa cgttcgatac ccaatggtgg
    25261 attgaggcac tgcacaaagc tattcctcag gcaagagtca gagcatggaa aagcggagac
    25321 aatgaatcag ccgattatgc tttagtctgg catcctcctg ttgaaatgct ggctgggcgc
    25381 gatcttaaag cggtgtttgc acttggggca ggtgttgatt ctattttgag caagctacag
    25441 gctcaccctg aaatgctgaa gccttctgtt ccactttttc gcctggaaga taccggtatg
    25501 ggcgagcaaa tgcaggaata tgctgtcagt caggtgctgc attggtttcg acgttttgac
    25561 gattaccgca tccagcaaaa tagttcgcat tggcaaccgc tgcctgaata tcatcgggaa
    25621 gattttacca tcggcatttt gggtgcaggc gtactgggca gtaaagttgc acagagtctg
    25681 caaacctggc gcttcccgct gcgttgctgg agtcgaaccc gtaaatcgtg gcctggcgtg
    25741 caaagctttg ccggacggga agaactctct gcatttctga gccaatgtcg ggtattgatt
    25801 aatttgttac cgaatacacc tgaaaccgtc ggcattatta atcaacaatt actcgaaaaa
    25861 ttaccggatg gcgcgtatct cctcaacctg gcgcgtggtg ttcatgtcgt ggaagatgac
    25921 ctgctcgcgg cgctggatag cggcaaagtt aaaggcgcaa tgctggatgt ttttaatcgt
    25981 gaacccttac cgccggaaag tccgctctgg caacatccac gcgtgacgat aacaccacat
    26041 gtcgccgcga ttacccgtcc cgctgaagct gtggattaca tttcgcgcac cattgcccag
    26101 ctcgaaaaag gggagagggt ctgcgggcaa gtcgaccgcg cacgcggcta ctaataaagc
    26161 atctggtttc ctgctatcct tggcgggaat tgaatacagg agagagttat gtatcccgtc
    26221 gaccttcata tgcataccgt tgccagcacg catgcatata gcacattgag tgattacatt
    26281 gcccaggcca aacaaaaggg cattaaactt tttgctatca ccgatcatgg cccggatatg
    26341 gaagatgcgc cgcatcactg gcacttcatt aacatgcgta tctggccgcg agtggttgat
    26401 ggggtaggga tcctgcgcgg catcgaagct aacattaaaa atgttgatgg tgaaattgac
    26461 tgcagcggca aaatgtttga ttcgctggat ctgattattg ccggttttca tgagccggtt
    26521 tttgcgccac atgacaaagc gaccaataca caagcgatga tcgccactat cgccagcggc
    26581 aatgtgcata taataagcca tcccggaaat ccgaaatatg agatagatgt taaagccgtt
    26641 gctgaagcag ccgcgaaaca tcaggtggcg ctggaaatca ataattcctc atttttacac
    26701 tcacgtaagg gcagtgaaga caactgtcgt gcgatagccg cagcggtacg tgacgctgga
    26761 ggttgggtgg cattaggctc ggattctcac actgcgttta ccatggggga atttgaagag
    26821 tgtcttaaaa tcctcgacgc ggtagatttt ccgccagagc gcattttgaa tgtttctccg
    26881 cgccgcttac ttaacttcct tgaatctcgc ggtatggcac cgattgcgga atttgcagac
    26941 ctttaattac tagtaatgga aatatattaa tgaatgagtt ttctatcctc tgtcgtgtgc
    27001 tgggttcgct ctattaccgc caaccgcaag atcctttact ggtgccgttg tttaccctga
    27061 ttcgtgaggg gaaactggct gcgaactggc cactggagca ggatgagtta ctgacgcgtt
    27121 tacagaaaag ctgtgatatg acgcaagtct ctgccgatta caatgcgttg tttatcggcg
    27181 atgaatgtgc tgtgccgcca tatcgtagcg catgggttga gggcgcaacg gaagcggaag
    27241 tgcgcgcttt tctttccgaa cgagggatgc cactagcgga tacgccagcc gatcacatcg
    27301 gcacattgct gcttgcggct tcctggctgg aagatcagtc aacggaagat gagagcgaag
    27361 cactggaaac actgttcagt gagtatctgt taccctggtg tggtgcgttc cttggcaaag
    27421 tggaggccca tgcaactacg cctttctggc gcaccatggc accgctaacc cgcgatgcta
    27481 ttagtgcaat gtgggacgag ctggaagaag attctgaaga gtaaatgtga tctgcatcac
    27541 tcataactgc aacgggtaat ttgttgttgc ataaaatgtg tgctcgatct cattcatggc
    27601 cgcgttttct gctatcatgc gcggcatgaa catacttctc tctattgcaa tcacaacagg
    27661 cattctctcc ggtatctggg gatgggtggc tgtttccctg ggcttactaa gctgggcggg
    27721 cttcctgggc tgtacggcct actttgcctg cccgcaaggt gggctgaaag ggctggcgat
    27781 ctccgctgca accctgctta gtggcgtggt gtgggcgatg gtcattattt acggtagtgc
    27841 attggcacca catctggaaa tactcggtta tgtcataacc ggtattgtcg cgtttctgat
    27901 gtgtatccag gccaaacagc tgctgctttc atttgttccg gggacgttta taggcgcatg
    27961 cgcgacgttt gccgggcagg gtgactggaa acaggtgtta ccttcgctgg cgcttggact
    28021 ggtatttggt tacgcaatga aaaacagcgg cctgtggttg gcggcgcgta gtgcaaagac
    28081 agctcaccgt gagcagcaaa tcaaaaataa agcgtgaggg gcactcacgc tttcgcttaa
    28141 accgtaaaat gccggatgat aattccggct tttttatcta tcaggattcc ggtggaaccg
    28201 acatatggcg gtatttcacc agaatgtcat tctgccgttc tgctttattt tgcaaatccc
    28261 acagaccacg gtcgatacca tcattaatca ggaaaatgac ccctgtttcg atagccgaca
    28321 tcaggcacag cataacaggt tcgttcgagg tgtaacccac ttccccttcc agcaagcgct
    28381 ggtagtcaat aaagcggaaa accccagcct gaacttcata agaaagtatc gtcttactgg
    28441 tgttcaccga ggagaggatc tcaccggtac tcacattgac gacgcgcagg ttcacggcaa
    28501 tctgatcgag ctggtattgc gtgtcggcac cgattccaaa atatcttgcc ccaaccccgc
    28561 ccgatttgac gttgctttca taaccgataa tcgaaccttc aaccatgata tttgccgccg
    28621 ttaaagattg cagcgggatt cggttattaa tggcaactgt gccgttttct tgtgccgcac
    28681 gaataatctt gcgctcgtta agcagatttt gtaagccctg gcgctccagc ggtataaacc
    28741 agcgagaatc tttcaatgcc gtgaccagca ttgccgtggc gctttgcgga acagcagtgg
    28801 agaagttact tgccgggtag ggtttaaatt gcccggtttc gtcctgaatg ttgtataccg
    28861 aaacaaagat tttacccgtc ggcgctggca gatgggtcaa atctttgtag ctctgagcac
    28921 gaggcattaa tgtcggtctg gcggcttctt taggcggggc ggttaagcat ccgctcagta
    28981 acatgacggc aaccaaaaga aataagcgct gcatgattat tttccttatg aagctggggc
    29041 ttaaaaatcg gttgagttat tttgtaaacc cgaaacctgg atggtcgagg tttgtccggt
    29101 tttacgatct gtcacgttca actgcaattg accatcgcgg ttagcaatat caacaatata
    29161 atcgttggtc accatgcggc ccggtttacc ggtattaata ttcgacagta gcccacctaa
    29221 aatttgtgac tggatggcct gagtaaagtt atctaacgct gatggcgttt caataccaaa
    29281 gtcatcgtta tagctcggat ctttataaga gttttgggcc tgagcgctat ttaacaaaaa
    29341 agcgccatta tttgggttac caccaaagtt tggattacgg aactggaaag tcatggttcc
    29401 agcccaactt aatggcgaaa taagcatgag tagaactact gcatgtttga cacgcattgc
    29461 agcctccgaa caatttatta tttaaaattc atcatgcgcc aaatcgcccg tacttaataa
    29521 cgcctggttt atctggcgac gatttagtgc ttcttcagtt tgaatcagtg caaagacgac
    29581 agttttctcg aagtctcttt tcaacggaaa taaaaaagtc tggaaaataa cgtcctgatt
    29641 caccgttatt gtgatccagc ttccccatcg tgcactgggc ctttcattaa tcgttaagtt
    29701 acccgtatag tcactttccc atttatcact aaaggctcgg taaaaatcat ggccaataga
    29761 tgaaacagta tggtcagtta acaatcccgg gacttctacc tcaacggcgt gaagattccc
    29821 tgcggcgaac agaaattctg ccgccacaat ccagcgtaaa taacgtttca tggctttatc
    29881 gcctgaggtt atcgtttgcc caggaaaccg cttgtgtccg gttttttacg gctatcttct
    29941 tgaaaagatt ataaagatgc gttttaaccg tattttcgct gatgaacaac gaacgagcga
    30001 tctcgttatt agacgcgccg atacgcagct tattcaggat ctctttttcc cgatgagtaa
    30061 ggagggctga ttccgtgctg ttataacgat agttacctga atgcgtaatc aggtaactgg
    30121 caagcttttg cgtaaagtag cattcgccgc gcaggacgcc ttgcaaccca ttgacaacac
    30181 gttcttgatc ctccatggca taaaaaacgc cgttgatatg aggccagttt tcaatgtcgc
    30241 ggtacgggta atcttcaggc gtatttagca acaatatttt gatattgttg tttttcctgc
    30301 tcaaagtatc ctgccaataa tggataagct ttttatccgc ttccatcata tccagaagaa
    30361 taatagagcc tgaagatata tcgtccagag aacgttgaat attatgtaat tttcctgtaa
    30421 ttgccagcga ttgtttaagg tgctgcaaga gagctgtcgc ctgcaaagaa ggtttagtga
    30481 tcaacaataa tgtatgacca tgaatactat ggacttcatt aaacatgatg aaaccccgct
    30541 ttttttattg atcgcacacc tgacagctgc ctctaaaata gaagcaccag aagtactgac
    30601 agatgttgca ctgctgtgtg tagtaataaa tcagccctaa atgggtaaaa tataaaacta
    30661 atggattaca tctgatttca atctagccat tacaaatctt aaa
//
LOCUS       DADSYM010000044        29892 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2415
            SAMN08039579-rid11723173.denovo.044, whole genome shotgun sequence.
ACCESSION   DADSYM010000044 DADSYM010000000
VERSION     DADSYM010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039579
            Sequence Read Archive: SRR6362533
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 29892)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29892)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980162.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 03:39:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,484
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 4,347
            CDSs (with protein)               :: 4,347
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 43 of 85
            Pseudo Genes (incomplete)         :: 37 of 85
            Pseudo Genes (internal stop)      :: 19 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29892
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039579-rid11723173.denovo.044"
                     /strain="LGE2415"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8302 N 95.9628 W"
                     /collection_date="2015-08-24"
     gene            662..1750
                     /locus_tag="J1Y83_001674"
     CDS             662..1750
                     /locus_tag="J1Y83_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310954.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HBA7629825.1"
                     /translation="MSPQNNHLQRPPAAVLYADELAKLKQNDHAPCPPGWQLSLPAAR
                     AFILGDSAQNISRKVVISPSAVERMLVTLATGRGLMLVGEPGTAKSLLSELLATAISG
                     DAGLTIQGGASTTEDQIKYGWNYALLINHGPSTEALVPAPLYQGMRDGKIVRFEEITR
                     TPQEVQDCLLGMLSDRVMTVPELTGEASQLYAREGFNIIATANTRDRGVNEMSAALKR
                     RFDFETVFPIMDFAQELELVASASARLLAHSGIPHKVPDAVLELLVRTFRDLRANGEK
                     KTSMDTLTAIMSTAEAVNVAHAVGVRAWFLANRAGEPADLVDCIAGTIVKDNEEDRAR
                     LRRYFEQRVATHKETHWQAYYQARHRLP"
     gene            1761..4040
                     /locus_tag="J1Y83_001675"
     CDS             1761..4040
                     /locus_tag="J1Y83_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708008.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA7629826.1"
                     /translation="MSEPLIVGIRHHSPACARLVKLLIESQRPRYVLIEGPADFNDRV
                     DELFLAHQLPVAIYSYCQYQDGAAPGRGAWTPFAEFSPEWQALQAARRIQAQTYFIDL
                     PCWAQSEEEDESPDTQDDSQTLLLRATCMDNSDTLWDHLFEDESQQTALPSTLAHYFA
                     QLRSDSPGDALNRQREAFMARWITWAMQQNNGDVLVVCGGWHAPALAKMWRECPQEIN
                     KPELPSLADAVTGCYLTPYSEKRLDVLAGYLSGMPAPVWQNWCWQCGLQQAGEQLLKT
                     VLTRLRQHKLPASTADMAAAHLHAMALAQLRGHTLPLRTDWLDAIAGSLIKEALNAPL
                     PWSYRGVIHPDTDPILLTLIDTLAGDGFGKLAPSTPQPPLPKDVTCELERTGISLPAE
                     LTLNRFTPDGLAQSQVLHRLAILEIPGVVRQQGSTLSLAGNGEERWKLTRPLSQHAAL
                     IEAACFGATLQEAARHKLEADMLDAGGISIITACLSQAALAGLASFSQQLLEQLTLLI
                     AQENQFAEMGQALEVLYALWRLDEISGMQGAQILQTTLCAAIDRTLWLCESNGRPDEK
                     EFHTHLHSWQALCHILRDLHSGVNLPGVSLSAAVALLERHSQAIHVLTLDRGATLGAL
                     MRLEHPNASAEAALTMLAQLSPTQSGEALHGLLALARHQLACQPVFIAGFSSHLNQLS
                     DADFINALPDLRAAMAWLPPRERGTLAHQVLEHYQLVQLPVSALQMPLHCPPQDIAHH
                     QQLEQQALASLQHWGVFHV"
     gene            4033..5169
                     /locus_tag="J1Y83_001676"
     CDS             4033..5169
                     /locus_tag="J1Y83_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HBA7629827.1"
                     /translation="MSELNDLLTTRELQRWRLILGEAAETTLCGLDDNARQIDHALEW
                     LYGRDPERLQRGERYGGLGRSNLTTPEWINSIHTLFPQRVIERLESDAVLRYGIEDVV
                     TNLDVLERMQPSESLLRAVLHAKHLMNPEVLAAARRIVHQVVEEIMARLAKEVRQAFS
                     GVRDRRRRSFISLARNFDFKSTLRANLQHWHPQHGKLYIESPRFNSRIKRQSEQWQLV
                     LLVDQSGSMVDSVIHSAVMAACLWQLPGIRTHLVAFDTSVVDLTADVADPVELLMKVQ
                     LGGGTNIASAMEYGRQLIEQPAKSVIILVSDFYEGGSSSLLTHQVKKCVQSGIKVLGL
                     AALDSTATPCYDHDTAQALVNVGAQIAAMTPGELASWLAENLQS"
     gene            5166..7169
                     /locus_tag="J1Y83_001677"
     CDS             5166..7169
                     /locus_tag="J1Y83_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016242262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA7629828.1"
                     /translation="MNSLRPELLELTPQALTALSNAGFVKRSLKELENGNVPEISHEN
                     GALIATFSDGVRIQLANGQALKEAQCTCGASGMCRHRVMLVLSYQRLCATAQPTEKKE
                     EEWDSAIWLKELATLPDATRKRAQALVAKGITIELFCAPGEIPSARLPMSDVRFYSRS
                     SIRFARCDCIEGTLCEHVVLAVQAFVEAKTQQAEFTHLIWQMRSEHVTSSDDPFASEE
                     GKTCRQYVQQLSQALWLGGISQPPIHYEAAFSRAQQAAERCNWRWVSESLRQLRASVD
                     AFHARASHYHAGECLRQLAALNSRLNCVQEMARRDSIGEVPPMPWHTVVGAGIAGEAK
                     LDHLRLVSLGMRCWQDIEQYGLRIWFTDPDTGSILHLSRSWQRSEQENSPAATRRLFS
                     FQAGALAGGQIISQAAKRSADGELLLATRNRLSSVVPLSPDAWQMLSAPLRQPGIVAL
                     REYLRQRPPACIRPINQVDNLFILPIAECISLGWDSSRQTLDAQVISGEGEDNVLTLS
                     LPASACSPFAVERMAALLQQTDDPVSLVSGFVSFVEGQLTLEPRVMMTKTRAWALDAE
                     TAPVAPLPSASVLPVPPTAHQLLMRCQALLIQLLHNGWRYQEQSAIGEAELLANDLTA
                     VGFYRLAHVLGQFRNTESEARVEAMNNGVLLCEQLFPMLQQQG"
     gene            7294..7755
                     /locus_tag="J1Y83_001678"
     CDS             7294..7755
                     /locus_tag="J1Y83_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YehR family lipoprotein"
                     /protein_id="HBA7629829.1"
                     /translation="MKAFNKLFSLVVASVLVFSLAGCGDKEESKKFSANLNGTEIAIT
                     YVYKGDKVLKQSSETKIQFASIGATTKEDAARTLEPLSAKYKNIAGVEEKLTYTDTYA
                     QENVTIDMEKVDFKALQGISGINVSAEDAKKGIIMAQMELVMKAAGFKEVK"
     gene            complement(7796..8266)
                     /locus_tag="J1Y83_001679"
     CDS             complement(7796..8266)
                     /locus_tag="J1Y83_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1456 family protein"
                     /protein_id="HBA7629830.1"
                     /translation="MLSNDILRSVRYILKANNNDLVRILALGNVEATAEQIAVWLRKE
                     DEEGFQRCPDIVLSSFLNGLIYEKRGKDESAPALEPERRINNNIVLKKLRIAFSLKTD
                     DILAILTEQQFRVSMPEITAMMRAPDHKNFRECGDQFLRYFLRGLAARQHVKKS"
     gene            complement(8313..9032)
                     /gene="yehT"
                     /locus_tag="J1Y83_001680"
     CDS             complement(8313..9032)
                     /gene="yehT"
                     /locus_tag="J1Y83_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310963.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BtsR"
                     /protein_id="HBA7629831.1"
                     /translation="MIKVLIVDDEPLARENLRVFLQEQSDIEIVGECSNAVEGIGAVH
                     KLRPDVLFLDIQMPRISGLEMVGMLDPEHRPYIVFLTAFDEYAIKAFEEHAFDYLLKP
                     IDEARLEKTLARLRQERSKQDVSLLPENQQALKFIPCTGHSRIYLLQMKDVAFVSSRM
                     SGVYVTSHEGKEGFTELTLRTLESRTPLLRCHRQYLVNLAHLQEIRLEDNGQAELILR
                     NGLTVPVSRRYLKSLKEAIGL"
     gene            complement(9029..10714)
                     /gene="btsS"
                     /locus_tag="J1Y83_001681"
     CDS             complement(9029..10714)
                     /gene="btsS"
                     /locus_tag="J1Y83_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulatory system sensor histidine
                     kinase BtsS"
                     /protein_id="HBA7629832.1"
                     /translation="MYDFNLVLLLLQQMCVFLVIAWLMSKTPLFIPLMQVTVRLPHKF
                     LCYIVFSIFCIMGTWFGLHIDDSIANTRAIGAVMGGLLGGPVVGGLVGLTGGLHRYSM
                     GGMTALSCMISTIVEGLLGGLVHSILIRRGRTDKVFNPITAGAVTFVAEMVQMLIILA
                     IARPYEDAVRLVSNIAAPMMVTNTVGAALFMRILLDKRAMFEKYTSAFSATALKVAAS
                     TEGILRQGFNEVNSMKVAQVLYQELDIGAVAITDREKLLAFTGIGDDHHLPGKPISST
                     YTLKAIETGEVVYADGNEVPYRCSLHPQCKLGSTLVIPLRGENQRVMGTIKLYEAKNR
                     LFSSINRTLGEGIAQLLSAQILAGQYERQKAMLTQSEIKLLHAQVNPHFLFNALNTIK
                     AVIRRDSEQASQLVQYLSTFFRKNLKRPSEFVTLADEIEHVNAYLQIEKARFQSRLQV
                     NIAIPQELSQQQLPAFTLQPIVENAIKHGTSQLLDTGRVAISARREGQHLMLEIEDNA
                     GLYQPVTNASGLGMNLVDKRLRERFGDDYGISVACEPDSYTRITLRLPWRDEA"
     gene            10936..11667
                     /gene="mlrA"
                     /locus_tag="J1Y83_001682"
     CDS             10936..11667
                     /gene="mlrA"
                     /locus_tag="J1Y83_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MlrA"
                     /protein_id="HBA7629833.1"
                     /translation="MALYTIGEVALLCDINPVTLRAWQRRYGLLKPQRTDGGHRLFND
                     ADIDRIREIKRWIDNGVQVSKVKMLLSNENVDVQNGWRDQQETLLTYLQSGNLHSLRT
                     WIKERGQDYPAQTLTTHLFIPLRRRLQCQQPTLQALLAILDGVLINYISICLASARKK
                     QGKDALVVGWNIQDTTRLWLEGWIASQQGWRIDVLAHSLNQLRPELFEGRTLLVWCGD
                     NRTSAQQQQLTSWQEQGHDIFPLGI"
     gene            11727..11834
                     /locus_tag="J1Y83_001683"
     CDS             11727..11834
                     /locus_tag="J1Y83_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YohO"
                     /protein_id="HBA7629834.1"
                     /translation="MRIAKIGVIALFLFMALGGIGGVMLAGYTFILRAG"
     gene            complement(11815..12546)
                     /gene="yehW"
                     /locus_tag="J1Y83_001684"
     CDS             complement(11815..12546)
                     /gene="yehW"
                     /locus_tag="J1Y83_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter permease YehW"
                     /protein_id="HBA7629835.1"
                     /translation="MKMLRDPLFWLIALFVALIFWLPYSQPLFAALFPQLPRPVYQQE
                     SFAALALAHFWLVGISSLFAVIIGTGAGIAVTRPWGAEFRPLVETIAAVGQTFPPVAV
                     LAIAVPVIGFGQQPAIIALILYGVLPVLQATLAGLGSIDASVTEVAKGMGMSCSQRLR
                     KVELPLAAPVILAGVRTSVIINIGTATIASTVGASTLGTPIIIGLSGFNTAYVIQGAL
                     LVALAAIIADRLFERLVQAFSQHAK"
     gene            complement(12551..13477)
                     /gene="yehX"
                     /locus_tag="J1Y83_001685"
     CDS             complement(12551..13477)
                     /gene="yehX"
                     /locus_tag="J1Y83_001685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter ATP binding
                     protein YehX"
                     /protein_id="HBA7629836.1"
                     /translation="MIEFSHVSKLFGTQKAVNDLNLNFQEGSFSVLIGTSGSGKSTTL
                     KMINRLVEHDSGVIRFAGEEIRSLPVLELRRRMGYAIQSIGLFPHWSVAQNIATVPQL
                     QKWSRARIDDRIDELMALLGLEPDLRERYPHQLSGGQQQRVGVARALAADPQVLLMDE
                     PFGALDPVTRGALQQEMTRIHRLLGRTIVLVTHDIDEALRLAEHMVLMDHGEVVQQGN
                     PLTMLTRPANDFVRQFFGHSELGVRLLSLRSVADYVRREERAEGEALAEEMTLRDALS
                     LFVARGCEVLPVVNTQGEPCGTLHFQDLLEEA"
     gene            complement(13470..14627)
                     /gene="yehY"
                     /locus_tag="J1Y83_001686"
     CDS             complement(13470..14627)
                     /gene="yehY"
                     /locus_tag="J1Y83_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter permease YehY"
                     /protein_id="HBA7629837.1"
                     /translation="MTYFRINPVLALLLLLTAIAAALPFISYAPNRLVSGEGRHLWQL
                     WPQTLWMLVGVGCAWLTACFIPAKKGSIFALILAQFVFVLLVWGAGKAATQLAQNGSA
                     LARTSLGSGFWLAAALALLACSDAIRRISTHPLWRWLLHMQIAIVPLWLLYSGTLNDL
                     SLMKEYANRQDVFDDALAQHLTLLFGAVLPALVIGVPLGIWCYFSTARQGAIFSLLNV
                     IQTVPSVALFGLLIAPLAALVTAFPWLGTLGIAGTGMTPALIALVLYALLPLVRGVVV
                     GLNQIPRDVLESARAMGMSGAQRFLHVQLPLALPVFLRSLRVVMVQTVGMAVIAALIG
                     AGGFGALVFQGLLSSAIDLVLLGVIPVIVLAVLTDALFDLLIALLKVKRND"
     gene            complement(14634..15551)
                     /gene="osmF"
                     /locus_tag="J1Y83_001687"
     CDS             complement(14634..15551)
                     /gene="osmF"
                     /locus_tag="J1Y83_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter
                     substrate-binding protein OsmF"
                     /protein_id="HBA7629838.1"
                     /translation="MPLSKVWAGSLVLLAAVSLPLQAASPVKVGSKIDTEGALLGNII
                     LQVLESHGVPTVNKVQLGTTPVVRGAITSGELDIYPEYTGNGAFFFKDENDTAWKNAQ
                     QGYEKVKKLDAEQNKLIWLTPAPANNTWTIAVRQDVAEKNKLTSLAELSRYLKEGGNF
                     KLAASAEFIERADALPAFEKAYGFKLGQDQLLSLAGGDTAVTIKAAAQQTSGVNAAMA
                     YGTDGPVAALGLQTLSDPQGVQPIYAPAPVVRESVLKEYPQIAQWLQPVFASLDEKTL
                     QQLNASIAVEGLDAKKVAADYLKQKGWTK"
     gene            complement(15741..18038)
                     /gene="bglX"
                     /locus_tag="J1Y83_001688"
     CDS             complement(15741..18038)
                     /gene="bglX"
                     /locus_tag="J1Y83_001688"
                     /EC_number="3.2.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucosidase BglX"
                     /protein_id="HBA7629839.1"
                     /translation="MKWLCSVGIAVSLALQPALADDLFGNHPLTPEARDAFVTELLKK
                     MTVDEKIGQLRLISVGPDNPKEAIREMIKDGQVGAIFNTVTRQDIRAMQDQVMELSRL
                     KIPLFFAYDVLHGQRTVFPISLGLASSFNLDAVKTVGRVSAYEAADDGLNMTWAPMVD
                     VSRDPRWGRASEGFGEDTYLTSIMGKTMVEAMQGKSPADRYSVMTSVKHFAAYGAVEG
                     GKEYNTVDMSPQRLFNDYMPPYKAGLDAGSGAVMVALNSLNGTPATSDSWLLKDVLRD
                     QWGFKGITVSDHGAIKELIKHGTAADPEDAVRVALKSGINMSMSDEYYSKYLPGLIKS
                     GKVTMEELDDAARHVLNVKYDMGLFNDPYSHLGPKESDPVDTNAESRLHRKEAREVAR
                     ESLVLLKNRLETLPLKKSATIAVVGPLADSKRDVMGSWSAAGVADQSVTVLTGIKNAV
                     GENGKVLYAKGANVTSDKGIIDFLNQYEEAVKVDPRSPQEMIDEAVQTAKQSDVVVAV
                     VGEAQGMAHEASSRTDITIPQSQRDLIAALKATGKPLVLVLMNGRPLALVKEDQQADA
                     ILETWFAGTEGGNAIADVLFGDYNPSGKLPMSFPRSVGQIPVYYSHLNTGRPYNADKP
                     NKYTSRYFDEANGALYPFGYGLSYTTFTVSDVKLSAPTMKRDGKVTASVQVTNTGKRE
                     GATVVQMYLQDVTASMSRPVKQLKGFEKITLKPGETQTVSFPIDIEALKFWNQQMKYD
                     AEPGKFNVFIGTDSARVKKGEFELL"
     gene            18234..19949
                     /gene="dld"
                     /locus_tag="J1Y83_001689"
     CDS             18234..19949
                     /gene="dld"
                     /locus_tag="J1Y83_001689"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HBA7629840.1"
                     /translation="MSSMTTTDNKAFLNELARLVGHSHLLTDPAKTARYRKGFRSGQG
                     DALAVVFPGSLLELWRVLKACVTADKIILMQAANTGLTEGSTPNGNDYDRDIVIISTL
                     RLDKLHVLGKGEQVLAYPGTTLYSLEKALKPLGREPHSVIGSSCIGASVIGGICNNSG
                     GSLVQRGPAYTEMSLFARINEDGKLTLVNHLGIDLGETPEQILSKLDDDRIKDDDVRH
                     DGRHAHDYDYVHRVRDIEANTPARYNADPDRLFESSGCAGKLAVFAVRLDTFEAEKNQ
                     QVFYIGTNQPEVLTEIRRHILANFENLPVAGEYMHRDIYDIAEKYGKDTFLMIDKLGT
                     DKMPFFFNLKGRTDAMLEKVKFFRPHFTDRAMQKFGHLFPSHLPPRMKNWRDKYEHHL
                     LLKMAGDGVGEAKSWLVDYFKQAEGDFFVCTPEEGSKAFLHRFAAAGAAIRYQAVHSD
                     EVEDILALDIALRRNDTEWYEHLPPEIDSQLVHKLYYGHFMCYVFHQDYIVKKGVDVH
                     ALKEQMLELLQQRGAQYPAEHNVGHLYKAPETLQKFYRENDPTNSMNPGIGKTSKRKN
                     WQEVK"
     gene            complement(19988..20920)
                     /gene="pbpG"
                     /locus_tag="J1Y83_001690"
     CDS             complement(19988..20920)
                     /gene="pbpG"
                     /locus_tag="J1Y83_001690"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416638.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine endopeptidase"
                     /protein_id="HBA7629841.1"
                     /translation="MPKFRVSLFSLALMLAVPFAPQAVAKTVAATTASQPEIASGSAM
                     IVDLNTNKVIYSNHPDLVRPIASISKLMTAMVVLDARLPLDEKLKVDISQTPEMKGVY
                     SRVRLNSEISRKDMLLLALMSSENRAAASLAHHYPGGYKAFIKAMNAKAKSLGMNNTR
                     FVEPTGLSVHNVSTARDLTKLLIASKQYPLIGQLSTTREDMATFSNPTYTLPFRNTNH
                     LVYRDNWNIQLTKTGFTNAAGHCLVMRTVINNKPVALVVMDAFGKYTHFADASRLRTW
                     IETGKVMPVPAAALSYKKQKAAQMAAAGQTAQND"
     gene            complement(21094..21681)
                     /locus_tag="J1Y83_001691"
     CDS             complement(21094..21681)
                     /locus_tag="J1Y83_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014170891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YIP1 family protein"
                     /protein_id="HBA7629842.1"
                     /translation="MSHVWGLFSHPDREMQVINRENETISHHYTHHVLLMAAIPVICA
                     FIGTTQIGWNFGDGTILKLSWFTGLALAVLFYGVMLAGVAVMGRVIWWMARNYPQRPS
                     LAHCMVFAGYVATPLFLSGLVALYPLVWLCALVGTVALFYTGYLLYLGIPSFLNINKE
                     EGLSFSSSTLAIGVLVLEVLLALTVILWGYGYRLF"
     gene            21851..22429
                     /locus_tag="J1Y83_001692"
     CDS             21851..22429
                     /locus_tag="J1Y83_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HBA7629843.1"
                     /translation="MDLNTLISQYGYAALVIGSLAEGETVTLLGGVAAHQGLLKFPLV
                     VLSVALGGVIGDQVLYLCGRRFGGKLLRRFSKHQDKIERAQKLIQRHPYLFVIGTRFM
                     YGFRVIGPTLIGASQLPPKIFLPLNILGAFAWSLIFTTIGYAGGQVIAPWLHNLDQHL
                     KHWVWLILVVVLVVGVRWWLKRRGKKKPDHQA"
     gene            complement(22549..23310)
                     /locus_tag="J1Y83_001693"
     CDS             complement(22549..23310)
                     /locus_tag="J1Y83_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HBA7629844.1"
                     /translation="MAQVAIITASDSGIGKECALLLAQQGFDIGITWHSDEEGAKDTA
                     REVVSHGVRAEIVQLDLGNLPEGAQALEKLIQRLGRIDVLVNNAGAMTKAPFLDMAFD
                     EWRKIFTVDVDGAFLCSQIAARQMVKQGQGGRIINITSVHEHTPLPDASAYTAAKHAL
                     GGLTKAMALELVRHKILVNAVAPGAIATPMNGMDGGDVKPDAEPSIPLRRFGTTHEIA
                     SLVVWLCSEGANYTTGQSLIVDGGFMLANPQFNPE"
     gene            complement(23363..24889)
                     /gene="mdtQ"
                     /locus_tag="J1Y83_001694"
     CDS             complement(23363..24889)
                     /gene="mdtQ"
                     /locus_tag="J1Y83_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311052.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug resistance outer membrane protein
                     MdtQ"
                     /protein_id="HBA7629845.1"
                     /translation="MLLMYCHAKLKNISQHTVISAHLFLPDYSPMNRDSFYPAIACFS
                     LLLMLAGCAPMHETRQALSQQTPAAQVDTVLPTALKNGWPDSQWWLEYHDNQLTSLIN
                     NALQSAPDMQVAEQRIQLAEAQAKAIATQDGPQLDFSADMERQKMSAEGLMGPFALND
                     PAAGTTGPWYTNGTFGLTAGWHLDIWGKNRAEVTARLGTVKARAAEREQTRQLLAGSV
                     ARLYWEWQTQAALNTVLQQIEKEQNTIIATDRQLYQNGITSAVEGVETDINASKTRQQ
                     LNDVAGKMKIIEARLSALTNHQTKSLKLKPVALPKVASQLPDELGYSLLARRADLQAA
                     HWYVESSLSTIDAAKAAFYPDINLMAFLQQDALHLSDLFRHSAQQMGVTAGLTLPIFD
                     SGRLNANLDIAKAESNLSIASYNKAVVEAVNDVARAASQVQTLAEKNQHQAQIERDAL
                     RVVGLAQARFNAGLIAGSRVSEARIPALRERANGLLLQGQWLDASIQLTGALGGGYKR
                     "
     gene            25023..25106
                     /gene="yohP"
                     /locus_tag="J1Y83_001695"
     CDS             25023..25106
                     /gene="yohP"
                     /locus_tag="J1Y83_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YohP"
                     /protein_id="HBA7629846.1"
                     /translation="MKIILWAVLIIFLIGLLVVTGVFKMIF"
     gene            complement(25494..26444)
                     /gene="dusC"
                     /locus_tag="J1Y83_001696"
     CDS             complement(25494..26444)
                     /gene="dusC"
                     /locus_tag="J1Y83_001696"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(16) synthase DusC"
                     /protein_id="HBA7629847.1"
                     /translation="MRVLLAPMEGVLDSLVRELLTEVNDYDLCITEFVRVVDQLLPVK
                     VFHRICPELQNASRTPSGTLVRVQLLGQFPQWLAENAARAVELGSWGVDLNCGCPSKT
                     VNGSGGGATLLKDPELIYQGAKAMREAVPAHLPVSVKVRLGWDSGEKKFEIADAVQQA
                     GATELVVHGRTKEQGYRAEHIDWQAIGEIRQRLNIPVIANGEIWDWQSAQQCMAISGC
                     DAVMIGRGALNIPNLSRVVKYNEPRMPWPEVVALLQKYTRLEKQGDTGLYHVARIKQW
                     LSYLRKEYDEATELFQHVRVLNNSPDIARAIQAIDIDKLR"
     gene            26683..27081
                     /locus_tag="J1Y83_001697"
     CDS             26683..27081
                     /locus_tag="J1Y83_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HBA7629848.1"
                     /translation="MSKTLNIIWQYLRAFVLIYACLYAGIFIASLLPVTIPGSIIGML
                     ILFVLLALQILPAKWVNPGCYVLIRYMALLFVPIGVGVMQYFDLLRAQFGPVVVSCAV
                     STLVVFLVVSWSSQLVHGERKVVGQKGSEE"
     gene            27078..27773
                     /locus_tag="J1Y83_001698"
     CDS             27078..27773
                     /locus_tag="J1Y83_001698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidB/LrgB family autolysis modulator"
                     /protein_id="HBA7629849.1"
                     /translation="MMANIWWSLPLTLIVFFAARKLAARYKFPLLNPLLVAMVVIIPF
                     LMLTGISYDSYFKGSEVLNDLLQPAVVALAYPLYEQLHQIRARWKSIITICFIGSVVA
                     MVTGTSVALLMGASPEIAASILPKSVTTPIAMAVGGSIGGIPAISAVCVIFVGILGAV
                     FGHTLLNAMRIRTKAARGLAMGTASHALGTARCAELDYQEGAFSSLALVLCGIITSLI
                     APFLFPIILAVMG"
     gene            27903..28787
                     /gene="cdd"
                     /locus_tag="J1Y83_001699"
     CDS             27903..28787
                     /gene="cdd"
                     /locus_tag="J1Y83_001699"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HBA7629850.1"
                     /translation="MHPRFQTAFAQLADNLQSALEPILADKYFPALLTGEQVSSLKSA
                     TGLDEDALAFALLPLAAACARTPLSNFNVGAIARGVSGTWYFGANMEFIGATMQQTVH
                     AEQSAISHAWLSGEKALAAITVNYTPCGHCRQFMNELNSGLDLRIHLPGREAHALRDY
                     LPDAFGPKDLEIKTLLMDEQDHGYALTGDALSQAAIAAANRSHMPYSKSPSGVALECK
                     DGRIFSGSYAENAAFNPTLPPLQGALILLNLKGYDYPDIQRAVLAEKADAPLIQWDAT
                     SATLKALGCHNIDRVLLA"
     gene            28937..29656
                     /gene="sanA"
                     /locus_tag="J1Y83_001700"
     CDS             28937..29656
                     /gene="sanA"
                     /locus_tag="J1Y83_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane permeability protein SanA"
                     /protein_id="HBA7629851.1"
                     /translation="MLKRVFLSLLVLIGLLLLTVLGLDRWMSWKTAPYIYDELQDLPY
                     RQVGVVLGTAKYYRTGVINQYYRYRIQGAINAYNSGKVNYLLLSGDNALQSYNEPMTM
                     RKDLIAAGVDPSDIVLDYAGFRTLDSIVRTRKVFDTNDFIIITQRFHCERALFIALHM
                     GIQAQCYAVPSPKDMLSVRIREFAARFGALADLYIFKREPRFLGPLVPIPAMHQVPED
                     AQGYPAVTPEQLLELQKKQGK"
     gene            29659..>29892
                     /locus_tag="J1Y83_001701"
     CDS             29659..>29892
                     /locus_tag="J1Y83_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2542 family protein"
                     /protein_id="HBA7629852.1"
                     /translation="MDVQQFFVVAVFFLIPIFCFREAWKGWRAGAIDKRVKNAPEPVY
                     VWRAKNPGLFFAYMVAYIGFGILSIGMIVYLIFY"
BASE COUNT         7111 a         8055 c         7609 g         7117 t
ORIGIN      
        1 agtgaatccc caggagctta ctaaagtaag tgacaggggt gaacgaacgc agtcgcagca
       61 catgcaactt gaagtatggc gagtaaagct ttattaaagg gggaaacggt attccccctt
      121 agatgcgttt caatttgccc gttacttcaa caaatcaatc aaccgtaaca gagccactaa
      181 tagctccagt acagcaatga gcacggttaa cttttgtgtc agcgtgtcca tccgctctcc
      241 ttattaagga gcgcaacacc agcaccttac ccttacactg cctgtcgata ctggtttacg
      301 tatgttggta cgaaacgttg ctctccggat cagggcaccg cctgcggcac cacccgtcca
      361 gcagccagga cttaaaattg gtccgatccg gcacaacctc ccaacgggca ggattataaa
      421 tctcattcac tgaattgcaa tggagtgtgg atatggttat aatttattcg ttggtacgaa
      481 atgttgcaca atcagcaagt tagcgtcatt gtagtaaata aaagaacctg cctcaccagc
      541 aggttttttt atttactgtg atctgctttc cagatatttt tcgctcaaac aactaatgcg
      601 gcaaacattt attgcgcgta aaatatcgtt tatttcatta atacatttca gggatgaata
      661 tatgtcaccg cagaataatc atctgcagcg tccgcctgcc gctgtgttat acgccgatga
      721 actggcaaaa ttaaaacaaa atgatcacgc accttgcccg cccggttggc agttaagttt
      781 gcctgcggcc cgcgctttta tccttggcga tagcgcgcaa aatattagcc gtaaagtcgt
      841 gatcagtccc tccgctgtcg aacgcatgtt agtcactctc gctaccgggc gcggtttgat
      901 gttggtgggg gaacctggca ccgcaaaatc gctcctgtct gaattactgg caaccgctat
      961 tagcggcgat gccgggttaa ccatccaggg cggcgcatct accaccgaag atcaaatcaa
     1021 gtacggctgg aactacgccc tgcttatcaa ccatggtccg tcaacggaag cgctggtccc
     1081 tgccccgctt taccagggaa tgcgcgacgg caaaattgtc cgttttgaag agataacgcg
     1141 tacaccgcag gaagtacaag actgtctgtt aggtatgctt tctgacaggg taatgacggt
     1201 gccggaactc actggtgaag ccagccagct ctatgcacgg gaaggcttta atattattgc
     1261 cactgccaat acccgcgatc gcggcgtcaa tgaaatgagt gctgcgctaa agcgtcgttt
     1321 cgattttgaa actgtctttc cgattatgga tttcgcccag gagctggagc ttgtcgccag
     1381 tgcttcggcg cgtttgctgg cccatagcgg cattccgcat aaagtgcctg acgcggtact
     1441 ggagttactg gtgcgcacct ttcgcgatct gcgtgccaac ggcgaaaaga aaacgtcaat
     1501 ggatacacta acagcgatta tgtccaccgc tgaagccgtg aacgtggccc acgcggtggg
     1561 cgtccgcgcc tggtttttag cgaatcgagc aggtgagcca gccgatctgg tggactgtat
     1621 tgccggaacc atcgtcaaag ataacgaaga agatcgcgcg cgcctgcgcc gttacttcga
     1681 acaacgtgtt gctacacata aagaaactca ctggcaggct tattatcaag ctcgccaccg
     1741 cctgccgtga ggaaagatgt atgagcgagc cgttaattgt cggcatccgg catcatagcc
     1801 cggcctgcgc ccggctggtg aaattgttaa tcgaaagcca gcggccacga tacgtattga
     1861 ttgaaggccc ggctgatttt aatgaccggg tggacgaact gtttttagcc caccagcttc
     1921 cggtagctat ttacagttat tgccagtatc aggacggtgc ggcccccggg cgtggtgcct
     1981 ggacgccatt tgctgaattt tcgccggagt ggcaggcgct acaagccgca cgtcgtattc
     2041 aggcacaaac ttacttcatc gatttgcctt gctgggcgca gagtgaagaa gaagacgaat
     2101 cacccgatac gcaagatgac agccagacct tactgctgcg tgccacctgc atggataaca
     2161 gcgataccct gtgggatcac ttattcgaag atgaaagcca gcaaactgca ttaccctcta
     2221 cgctggcgca ctattttgct caactgcgga gcgattcccc cggagatgcg ctcaatcgtc
     2281 agcgcgaagc ctttatggct cgctggatta catgggcgat gcagcaaaac aatggcgacg
     2341 tgttagtcgt ctgcggcggc tggcacgctc ctgcactggc aaaaatgtgg cgcgaatgcc
     2401 cgcaggaaat taacaagcca gaattgccct cgctggcaga tgccgttact ggttgttatc
     2461 tcacacccta cagtgaaaag cgccttgatg tgctggcagg atacctttcc ggaatgcctg
     2521 ccccggtctg gcaaaactgg tgctggcagt gtggcttaca gcaggcaggt gaacagctgc
     2581 tgaaaacggt tcttactcgt ttgcgccagc acaaattgcc cgcttctacc gctgatatgg
     2641 ctgctgctca tctgcatgct atggcactgg cacagttgcg cggccataca ctacctctac
     2701 gcactgactg gctggatgcc atagcaggat cgctgattaa agaagccctg aatgcgccgt
     2761 tgccgtggag ctatcgcggc gttattcatc ccgataccga tccgattctg ctaacgttga
     2821 tagacacatt agcgggtgac ggattcggta aacttgcccc ttctacgcca caaccgcctc
     2881 tgccaaaaga tgtcacctgc gaactggaac gtaccggaat ctctcttccg gcggagctta
     2941 ccttaaatcg ctttaccccc gatggactgg cgcaaagtca ggtgttacat cggttggcaa
     3001 tactggagat cccaggggtt gtgcgccagc agggaagtac actgtcactt gcaggcaacg
     3061 gtgaagaacg ctggaaatta acccggccac ttagccagca tgcggcattg attgaagccg
     3121 catgctttgg cgccacactc caggaagccg cacgccataa attagaagcc gatatgctgg
     3181 acgcgggtgg aatcagcatc atcactgcat gtcttagcca ggcagcgtta gcgggtctgg
     3241 cgtctttcag tcaacaatta ctggaacaac tcacattatt aatcgcccag gaaaatcaat
     3301 tcgccgaaat gggccaggcg ctggaagtgc tatatgcctt atggcggctg gatgaaatta
     3361 gcggtatgca aggcgcgcag atattacaga cgacgttatg cgcggctatc gatcgcacac
     3421 tctggctatg tgaatccaac ggcagaccgg atgaaaagga gtttcacact cacctgcata
     3481 gctggcaggc gctttgccat atcctgcgcg atctacatag cggcgttaat ttacccggcg
     3541 tttctctttc tgcggcggta gccttactgg agcgacacag tcaggcgatt catgtcctga
     3601 cactggatcg cggcgcgact cttggtgcgc tgatgcgtct ggaacatccc aacgccagtg
     3661 ccgaagcggc gctgacgatg ctggcgcagt tatccccgac acaatccggc gaggcgctgc
     3721 acggtttgct ggcattagcc cgtcatcaac tggcctgtca gccggtattt atcgccggtt
     3781 tcagcagtca tttaaatcaa ctgagtgatg ccgattttat caatgccctg cccgatttac
     3841 gcgcggcgat ggcctggcta ccaccacgag aacgcgggac gctggcgcat caggtgcttg
     3901 agcattatca actggtgcaa cttcccgttt cggcactgca aatgccgttg cattgtccac
     3961 cacaagacat tgcacatcat caacaactcg aacagcaggc actggcatcg ctgcaacact
     4021 ggggagtttt ccatgtctga actgaacgat cttctgacca cccgtgagct acaacgctgg
     4081 cgattaattc ttggcgaagc ggcagaaacg acgctttgtg ggctggatga caacgcccgg
     4141 cagatagacc acgcgctgga gtggctgtat gggcgcgatc ctgaacggct ccagcgtggt
     4201 gaacgttacg gtggattagg tcgttcaaat ctcaccaccc ctgagtggat caacagcatt
     4261 cacacgctgt ttccgcaacg ggtgatcgag cggctggaaa gcgatgccgt gctgcgctac
     4321 ggcattgaag atgtggtgac gaatctcgac gtgctggaac gtatgcagcc ttctgaaagc
     4381 ctgctacgcg ccgttttgca cgccaaacat ctgatgaatc ccgaagtact ggctgccgcc
     4441 cgccggatag tgcaccaggt tgttgaagag attatggcgc gactggcaaa ggaagttcgt
     4501 caggcttttt ctggagtacg cgatcgccgt cgccgctcat ttatttcact ggcgcgaaac
     4561 tttgatttca aaagtactct gcgcgccaac ctgcaacact ggcacccgca acacggcaag
     4621 ttgtatatcg aatccccccg ctttaacagc cgcattaaac gccaaagcga acaatggcaa
     4681 ctggtcttac tggttgatca aagcggatca atggtggact cggtgatcca ctccgcggtg
     4741 atggcggcct gtttatggca gttacccggt attcgtaccc atctggtggc gtttgacacc
     4801 agcgtggttg atctcacggc agacgttgcc gatccggtag agttattaat gaaagtgcaa
     4861 ttaggcggcg gaaccaatat cgccagtgcc atggaatatg gtcggcaact tattgaacaa
     4921 ccagcgaaaa gcgtcattat cctcgtgagc gatttttacg aagggggttc atcatcatta
     4981 ctgacgcatc aggtgaaaaa gtgtgtccag agcggcatca aagtgctggg actggcggcg
     5041 ctcgatagca ccgcaacacc ttgctatgac cacgatacgg cccaggcgct ggtaaatgtc
     5101 ggcgcacaaa tagccgccat gacaccgggc gagctggcat catggcttgc ggagaatctt
     5161 cagtcatgaa ttcactacgt ccggaattat tagaactgac gccgcaggcc ctgacggcgt
     5221 taagcaatgc cggttttgtt aagcgcagcc ttaaggaact ggaaaatggc aacgtcccgg
     5281 aaatcagtca tgagaacggc gctttaatcg ccaccttcag tgacggtgtc cgtatccagc
     5341 tggcgaacgg ccaggcactg aaagaggctc agtgcacctg cggggccagc ggcatgtgcc
     5401 gtcatcgcgt gatgctggtg ttaagttatc aacgactttg tgccaccgcc cagcctacgg
     5461 aaaaaaaaga agaagagtgg gattcggcaa tctggctgaa agaactggct accttgcccg
     5521 atgccacccg caaacgcgcg caggcgctgg tcgctaaagg catcaccatt gagttgttct
     5581 gtgcgcctgg cgaaattccc tctgcccgat taccgatgag cgatgtgcgt ttttattccc
     5641 gcagcagcat tcgcttcgca cgctgtgatt gcattgaagg cacactttgc gaacatgtcg
     5701 tactggctgt gcaggccttc gtcgaggcca aaactcagca agcggaattt actcatttaa
     5761 tctggcagat gcgcagtgaa cacgtcacat catctgatga tccgtttgcc agcgaagaag
     5821 gcaaaacgtg tcgtcaatat gttcagcaat taagccaggc attatggctg ggcgggataa
     5881 gtcaaccgcc aatccattac gaggccgctt tcagtcgcgc gcaacaagcg gcggaacgct
     5941 gcaactggcg atgggtgagt gaatcgctac ggcaattgcg cgccagtgtt gatgccttcc
     6001 acgcccgtgc cagccactac catgccggag aatgcttacg tcagcttgcg gcattaaaca
     6061 gtcgattaaa ttgcgtacaa gagatggcgc gacgcgacag tatcggtgaa gttcctccca
     6121 tgccgtggca cacggtcgtt ggcgctggta ttgccggaga agcaaaactt gatcatctgc
     6181 ggctggtgtc tttaggtatg cgttgctggc aggatattga gcagtatggt ttacgcatct
     6241 ggtttaccga tcccgacaca ggaagtattt tgcatctttc gcgtagttgg cagagaagcg
     6301 aacaggaaaa ctcaccggca gcgacgcgtc ggctgtttag ttttcaggct ggcgcactgg
     6361 ccggtgggca aattatttca caagcagcaa aacgcagtgc cgatggcgag ctgctgttag
     6421 ctacccgcaa ccgcttaagc agcgttgtgc cgctgtcgcc tgatgcctgg caaatgttga
     6481 gcgcaccgtt gcgccagccg ggcattgtgg ctttgcggga atatttacgc cagcgtcccc
     6541 ccgcctgcat acgaccaatc aaccaggtcg ataacttatt tattctgccg atagcggagt
     6601 gtatttcgct cggttgggat agcagccgcc agacgctgga tgcgcaggta atcagcggcg
     6661 aaggggaaga taatgtgctg acgttatccc ttccggcatc tgcctgttct ccttttgccg
     6721 ttgaacgcat ggcggcgctt ttgcaacaaa cagacgaccc cgtgagtctg gtttctggct
     6781 ttgtcagttt tgtagagggg caattgacac tggaaccacg ggtgatgatg acaaaaaccc
     6841 gcgcctgggc gctggacgca gaaactgcgc ctgtggcacc gctaccttct gccagcgttc
     6901 tgcctgtgcc gcctaccgct catcagttgc tgatgcgctg ccaggcgtta cttattcaac
     6961 tgctccataa cggctggcgc tatcaggaac agagtgctat tggtgaggca gagttgctgg
     7021 cgaatgacct caccgcggtg ggtttttatc ggctggcaca tgtgttggga caatttcgta
     7081 atacagaaag cgaggcacgg gtagaagcaa tgaataacgg tgttttgctt tgcgaacaat
     7141 tattccccat gcttcagcaa caaggatgaa atagtgcttt ttattaagag ttctactcct
     7201 gttccggact gctcacgcca cagtattagg catatcctat atagcccctg gtgagagtca
     7261 ccagttcctt gattaaataa aatggagttt tacatgaagg ctttcaataa gctgttttcc
     7321 cttgttgttg catctgttct ggttttctct cttgctggct gcggtgacaa agaagaatca
     7381 aagaaattca gcgccaatct gaacggcact gaaattgcca ttacctatgt ctacaaaggt
     7441 gacaaggtgc ttaagcaatc ttctgaaacc aaaattcaat ttgcctccat tggtgcaacc
     7501 accaaagaag atgccgccag gacacttgag ccgttaagcg ctaaatacaa aaacatcgcg
     7561 ggtgttgaag aaaaattaac ctataccgat acctacgcgc aggaaaacgt gactattgat
     7621 atggaaaaag tggattttaa agccctgcag ggtatttcag gaatcaacgt ttctgctgaa
     7681 gatgccaaaa aaggtatcat tatggcgcaa atggaactgg tgatgaaagc cgctggtttt
     7741 aaagaagtga aataatctgt cggcggccat gtttcgcatg gccgccatac ccgtcttagc
     7801 ttttcttcac atgctggcgc gctgccagtc cacgcagaaa ataacgtaaa aattgatcgc
     7861 cgcattcgcg gaagttttta tgatccggtg cacgcatcat cgccgtaatt tccggcatcg
     7921 aaacgcggaa ctgctgttcg gtgaggattg ccagaatgtc atcggtttta agcgaaaacg
     7981 cgatgcgtaa ttttttcagc acgatgttgt tattaatgcg acgttccggc tccagtgccg
     8041 gagcagactc atccttgccg cgtttttcat aaatcaggcc attgaggaat gaagacaaaa
     8101 caatgtccgg acaacgctga aaaccctctt cgtcttcttt acgtagccag acggcgatct
     8161 gttccgcggt ggcttcgaca ttacccagcg ccagaatacg caccaggtca ttattattgg
     8221 ctttcaaaat gtagcgcacg ctgcgcagaa tatcgttact tagcatgagg ccttcaggtg
     8281 ttgatgaggc aaaaagccat tttagcagtc ttttacaggc caatcgcctc ttttaagctt
     8341 ttcagataac ggcggctgac cggcacggtt aagccattac gcaaaatcaa ctcggcctgg
     8401 ccgttatctt ccagacgaat ctcctgtaaa tgcgcgaggt taaccagata ctgacgatgg
     8461 cagcgcagta gtggtgtacg actttccagg gtacgtaatg tcagttcggt aaagccctct
     8521 ttcccttcgt gactggtaac gtagacaccg ctcatccgac tgctgacaaa tgccacatct
     8581 ttcatttgca gcaaataaat ccgactatgc cccgtacaag ggataaattt cagcgcctgt
     8641 tgattttccg gtaacagcga aacatcctgc ttgctgcgct cctgacgcaa tcgcgccagc
     8701 gttttctcca gtcgcgcttc atcaattggc ttcagcagat aatcaaaggc atgttcttca
     8761 aaggctttaa tggcgtattc gtcaaacgcg gtgagaaaaa caatatacgg gcgatgttcc
     8821 ggatcaagca tccccaccat ttccagacca ctgatgcgcg gcatctggat atcgagaaac
     8881 agcacatccg ggcgcagttt atgcaccgcg ccgatccctt ctacggcgtt tgaacactct
     8941 ccaacgattt caatatcgct ctgctcttgc aaaaatacgc gcaggttctc ccgtgctaac
     9001 ggttcatcat cgacaattaa gactttaatc atgcctcgtc cctccatggt agtcgtaacg
     9061 ttattcgggt gtaactatca ggctcacagg cgacgcttat cccatagtca tcgccaaacc
     9121 gttcacgtaa acgcttatcc accagattca tccccagccc actggcattg gttaccggtt
     9181 gatacaaacc ggcattgtct tcgatctcca gcatcaaatg ttgcccctca cgtcgggcgc
     9241 tgattgccac tcgccctgta tccaacagtt gtgatgtccc atgtttaatg gcgttttcca
     9301 ctatcggttg cagggtaaac gcgggcaatt gctgctggga taattcttgc ggaatagcaa
     9361 tgttgacctg caaccgcgac tggaagcgcg ccttttcaat ttgcagataa gcgttcacat
     9421 gttcaatttc gtcggcgaga gtaacaaact ccgaaggccg ctttaagttt ttgcggaaaa
     9481 aagtggaaag atactgcacc agctggctgg cctgttcgct gtcgcggcgg atcaccgctt
     9541 taatggtgtt aagcgcatta aacaaaaaat ggggattcac ctgggcgtga agcagtttga
     9601 tctctgactg ggtgagcatc gctttttgcc gctcatattg cccggcgagg atctgcgccg
     9661 aaagcagttg cgcaatcccc tcgcccagcg tgcggttgat tgaactgaat aaacggtttt
     9721 tggcctcata caatttgatg gtacccatca ctcgctgatt ttcaccacgc aacggaatga
     9781 ccagcgtcga ccccagtttg cattgcggat gcaaagagca acggtaaggc acttcgttgc
     9841 catcagcgta gaccacttca ccggtttcaa tcgcttttaa ggtataagtc gaagaaattg
     9901 gtttgccggg taaatggtgg tcatcaccaa ttccggtaaa ggccagcaat ttctctcgat
     9961 cggtaatcgc gactgcacca atatccagct cctgatacag cacctgtgcc actttcatgc
    10021 tgttcacttc gttaaacccc tggcgcaaaa tgccttccgt cgaggccgcc actttcagcg
    10081 cagtggcaga aaaagccgaa gtgtattttt caaacatcgc gcgtttatcg agcaatatac
    10141 gcataaacaa cgccgcgccg acggtattgg tgaccatcat tggcgcagca atattactca
    10201 ccagacgcac cgcatcttca taaggtcggg cgatcgccag gatgatcaac atttgcacca
    10261 tttcagcgac gaacgtgacg gcaccggcgg taatggggtt aaagacttta tcagtgcgcc
    10321 cgcgacggat aaggatgctg tgtaccaggc cgccgagcaa tccttcaacg atggtcgaga
    10381 tcatgcaact tagcgcggtc atgcccccca tcgaatatcg atgtaacccg ccggtcagac
    10441 caaccagccc accgacgacc ggaccgccga gtaagccgcc cattaccgca cctatcgcac
    10501 gggtattggc aatagaatcg tcaatgtgca acccaaacca ggtgcccatg atgcagaaga
    10561 tggaaaagac gatgtagcag agaaatttat gcggcagacg aaccgtgacc tgcattaacg
    10621 gtatgaataa tggcgtttta ctcattaacc acgcaatgac taaaaaaacg cacatctgct
    10681 gaagcagcag caacaccaga ttaaaatcgt acatacccgc aaaccacact tccctttaaa
    10741 acgcgtaaca tacattgctt gcgtttaact ttctttgaac tcttgcagaa aaatgagaat
    10801 tcgtgagtac gatcactcaa aatcgcctgg caaaaataaa atcaccctat agatgctcaa
    10861 aaaacgggca aaactacctg gttcgcaaaa ctgcgtctaa agttaaaccg ggacctcgcg
    10921 agcaagggtg agacgatggc gctttacaca attggtgaag tggcgttgct ttgtgatatt
    10981 aaccctgtca cgttacgcgc gtggcagagg cgttacggat tgctgaaacc gcaacggaca
    11041 gacggcggtc atcggctgtt caacgatgcc gatattgacc ggatccgcga gatcaaacgc
    11101 tggatcgaca acggcgtgca ggtcagcaaa gtaaaaatgc tgctcagtaa tgaaaatgtt
    11161 gatgtgcaga acggctggcg cgatcagcaa gaaacattac tgacttacct gcaaagcggc
    11221 aatctgcata gcctgcgaac gtggatcaaa gagcgcggtc aggattaccc cgcccagaca
    11281 ctcaccacac atctgtttat tcctctgcgc cgacggcttc agtgccaaca accgactctc
    11341 caggcgctgc tggcgatcct cgacggcgta ctgatcaact acatctctat ttgcctggct
    11401 tcggcgcgca aaaaacaggg taaagatgca ctggtggttg gctggaatat tcaggatacc
    11461 actcgtctgt ggctggaggg ctggattgcc agtcaacaag gatggcgcat tgatgtcctc
    11521 gcccactcgc tcaatcaact acgccctgaa ctgttcgaag gccgtacatt gctggtatgg
    11581 tgcggtgata atcgaacctc cgcccaacag cagcaactca ccagttggca ggaacaaggc
    11641 catgatattt tcccactcgg catttaatga ttcgttaaca aatgcgcttt actgtacaat
    11701 cctttcgtta acataaggag tgcattatgc gcatagctaa aattggggtc atcgccctgt
    11761 tcctgtttat ggcgttaggc ggaattggtg gcgtcatgct cgcaggttat acctttattt
    11821 tgcgtgctgg ctgaacgcct gcaccagcct ttcaaacagg cggtctgcga tgatcgccgc
    11881 cagtgccacc agtaacgccc cctggatcac atacgcggta ttaaatccgc taagcccgat
    11941 gatgatcggc gtacccagcg tgctggcccc taccgttgag gcgatcgtcg ccgtaccaat
    12001 gttgataatc accgaagttc gcacgcccgc cagaatcacc ggagccgcca gcggtagctc
    12061 gaccttacgc agtcgctgac tacagctcat tcccatgcct ttggcaactt cggtcacgct
    12121 ggcatcaatc gatcccagcc cggcaagtgt cgcctgcagg acgggcagca caccgtaaag
    12181 gatcaaggcg ataatcgccg gttgctgacc aaagccgatc accggaacgg caatcgccag
    12241 cactgcaacg ggcggaaaag tctgtccaac ggcggcaata gtttccacca gtggacgaaa
    12301 ttccgcgccc caagggcgag tgacagcaat tccggcacca gtaccaatga tcaccgcaaa
    12361 caaactcgaa attcccacca gccagaaatg agccagtgcc agagctgcaa agctttcctg
    12421 ctgataaacg ggtcgtggca gttgtgggaa caaggcagca aacagcggct ggctgtaagg
    12481 cagccagaaa atcagcgcca caaacagagc aatgagccag aacagcggat cgcgcaacat
    12541 cttcatacgc ttacgcctcc tccagcagat cctgaaaatg cagcgtgccg caaggctcgc
    12601 cctgcgtgtt caccaccggc agcacttcac atccccgcgc gacaaacagg gagagcgcat
    12661 cgcgtagcgt catctcttct gccagcgcct caccttctgc ccgttcttcg cgacgcacgt
    12721 aatccgccac gctacgtaac gaaagcaggc ggacacccag ttcactatgc ccaaaaaact
    12781 ggcggacaaa atcattcgcc ggacgagtca gcatcgtcag cggattgccc tgctgcacca
    12841 cttcaccgtg atccatcaac accatatgtt ctgccagtcg tagcgcctca tcaatatcat
    12901 gagtgaccag cacaatggta cgccccagca aacggtgtat gcgcgtcatc tcttgttgca
    12961 acgcgccacg cgttaccggg tccagtgcgc cgaatggctc atccatcagc aagacttgcg
    13021 gatcggcagc cagcgcgcgg gccactccca cacgttgctg ctgaccgccg gaaagctgat
    13081 gcggataacg ctcacgcaaa tccggctcca gccccagtag cgccattaat tcatcgatac
    13141 gatcgtcgat ccgcgcccgc gaccattttt gtaattgcgg cacggtggca atattttgcg
    13201 ccacgctcca gtggggaaac aggccaatag attgaatggc atagcccatc cggcggcgca
    13261 actccagcac tggcagcgag cgaatttctt ctccggcaaa gcggatcacg ccactgtcat
    13321 gctccaccag gcggttaatc attttcagag tggtggattt accggagcct gatgtaccaa
    13381 tcagcaccga aaaactcccc tcctgaaaat tgagattgag atcgttaacg gctttttgtg
    13441 tgccgaacag tttgctgaca tggctaaatt caatcattac gtttcacctt cagcagtgcg
    13501 ataagcaaat cgaacagcgc gtcggtaagc accgccagaa caattaccgg gatcaccccc
    13561 agcaacacta aatcaatggc actgcttaac agcccctgaa aaaccagcgc accaaaaccg
    13621 cctgcgccga ttaacgccgc aatcaccgcc atgccaacgg tttgcaccat caccacccgc
    13681 aggctgcgca gaaataccgg taacgccagc ggcaactgaa catgcaggaa acgctgcgcc
    13741 ccgctcatcc ccatcgctct ggcgctctcc agcacatcgc gcgggatctg gtttaagccg
    13801 actaccacgc cgcgcaccag cggcagcaag gcatagagca ccagcgcaat cagtgcgggt
    13861 gtcattccgg ttcctgctat gccgagcgtt cccagccacg gaaaggccgt aaccagcgcg
    13921 gcaagcggcg caatcagcag gccaaagagc gctaccgaag gcacggtctg aatgacattc
    13981 agcagagaaa aaatcgcccc ctgccgcgcg gtggaaaagt agcaccagat gcccaacggc
    14041 acaccaatca ctaacgcagg cagcaccgca ccaaacagca acgtcagatg ttgtgccagc
    14101 gcgtcgtcaa acacatcctg acggttggcg tattctttca ttaacgagag atcgttaagc
    14161 gtgccggagt acagcaacca cagcggaaca atggcaatct gcatatgcaa caaccagcgc
    14221 cacagcggat gcgtggagat tcggcggatg gcatcgctac aggccagcag tgccagcgcc
    14281 gcagccagcc agaaaccact gccgaggctg gtacgcgcca gcgcactgcc attttgcgcc
    14341 agttgtgtcg ccgcctttcc agctccccac accagcaata cgaagacgaa ttgcgccaga
    14401 atgagtgcaa aaatgctgcc ttttttagcg ggaataaaac aggctgtcag ccaggcgcaa
    14461 ccaacgccca ccagcatcca gagagtttgc ggccacagct gccagagatg acgcccctca
    14521 cccgaaacta aacgattagg tgcgtaactg ataaacggca gcgccgctgc gattgccgtc
    14581 agcaacagca gcagcgccag aacaggatta atacggaaat aagtcacggg aaattacttc
    14641 gtccaccctt tttgtttcag gtagtcggct gccacttttt tggcatccag tccttccact
    14701 gcaatgctgg cattcagctg ctgcaatgtt ttttcatcga ggctggcgaa gactggctgt
    14761 agccactgtg ctatttgcgg atactctttc agcaccgact cacgcaccac tggcgcaggc
    14821 gcgtagatag gttgcacgcc ttgcggatcg cttaaggttt gcagccccag cgccgcaacc
    14881 gggccgtcgg tgccgtaagc cattgcggca ttaacaccag aagtttgctg ggcagcggct
    14941 ttgatcgtca ccgccgtgtc accaccggcc agtgacagca actgatcctg accgagctta
    15001 aagccatagg ctttttcaaa cgcgggtaag gcatcggcgc gttcgataaa ctctgccgag
    15061 gctgccagtt tgaagttgcc accctctttc agataacgac tcagctcggc aagcgaagtg
    15121 agtttgtttt tctctgccac atcctgacgc acggcgatgg tccaggtgtt atttgcaggc
    15181 gcgggcgtca gccagattaa cttgttttgc tctgcatcga gttttttgac tttctcatag
    15241 ccttgctgcg cgtttttcca tgccgtatcg ttttcatctt taaagaaaaa cgcgccattg
    15301 ccagtatatt ccggatagat atccagctcg ccagaagtga tcgccccgcg cactacggga
    15361 gtcgttccca gctgcacttt atttaccgta ggaacgccgt gactttccag cacctgcaaa
    15421 atgatattgc cgagcagcgc gccttccgta tcgatttttg aaccaacctt aacgggggaa
    15481 gccgcctgta gcggcaggct cacggctgcc aacaaaacca gtgaacctgc ccagaccttt
    15541 gagagtggca tgatgctttc ctcattcttt tactgttgtt ttcagcgaat taagagaaaa
    15601 gcatagttga taatggcgga gttagcctga ctgggtggac tcagttgcag aatcagataa
    15661 atacttaaaa ggcatatttt gcccggcgtt tcttgccgga tgcggcgtaa acgccttatc
    15721 cggcctacaa acatcgaaaa ttacaacaac tcaaactcgc ctttcttaac gcgtgcggaa
    15781 tcagtgccga tgaagacatt gaacttgcca ggctcggcgt catatttcat ctgttgattc
    15841 cagaacttca gcgcctcaat atcgattggg aagctgacgg tctgagtttc gcccggtttc
    15901 agggtgattt tctcaaagcc tttcagctgc ttcaccgggc gactcatgga agccgtcaca
    15961 tcctgcaagt acatctgtac taccgtggcc ccttcgcgtt taccggtgtt cgtcacctgc
    16021 acgctggccg tcactttacc gtcacgcttc atggtcggcg cagaaagttt cacatcagag
    16081 acggtgaaag tggtgtagct cagcccatag ccgaacggat aaagcgcccc gttagcttca
    16141 tcaaaataac gcgaagtgta tttgttcggc ttgtcggcat tatacggacg cccggtattc
    16201 agatggctgt agtacaccgg gatctgcccg acagaacgcg ggaaggacat cggcagcttg
    16261 ccggacgggt tataatcgcc aaacaataca tcggcaattg cattaccgcc ttcagtcccc
    16321 gcaaaccagg tttccagaat cgcatcagcc tgctgatctt ctttcaccag cgccagcgga
    16381 cgcccattca tcagcaccag caccagcggt ttaccggtgg ctttcagcgc cgcaatcaag
    16441 tcacgttggc tttgcggaat agtgatatcg gtacgactgg aagcttcgtg cgccatcccc
    16501 tgagcttcac cgaccacagc caccaccaca tcagattgtt tcgcggtttg caccgcttca
    16561 tcaatcatct cttgcggcga acgcggatca actttgaccg cctcttcata ctgattcagg
    16621 aaatcgataa tgcctttgtc actggtaacg ttcgcccctt tggcatacag cactttaccg
    16681 ttctcaccga cggcgttttt aatccctgtt agcacagtaa cagattgatc ggcgacacct
    16741 gctgccgacc agcttcccat cacgtcacgc ttgctgtctg ccagcgggcc aaccaccgca
    16801 atggttgcag attttttcag cggcagcgtt tcgagacggt ttttcagcaa caccaggctt
    16861 tcgcgcgcca cttcacgcgc ttctttacgg tgtaggcggc tttcggcatt ggtatccacc
    16921 gggtcagact ctttcggccc caaatggctg tatgggtcgt taaacaatcc catatcatat
    16981 ttaacgttca gtacatggcg ggcagcatcg tccagctctt ccatcgtcac tttgccggat
    17041 ttgatcaacc caggcagata cttcgagtaa tactcgtcgc tcatgctcat gttgattccg
    17101 gacttcagcg ccacgcgtac cgcatcctct ggatctgccg ccgtgccatg tttaatcagc
    17161 tctttgattg cgccgtgatc ggaaacggtg atgcctttaa agccccactg gtcgcgcaga
    17221 acatctttca gcagccagga gtcggaggtg gctggcgtgc cgttcagcga gttcagcgcc
    17281 accatcaccg caccgctgcc tgcgtccagc cccgctttgt acggcggcat atagtcgtta
    17341 aacaggcgct gcggactcat atcgacggtg ttgtactctt taccgccttc taccgcgccg
    17401 tatgcggcaa agtgtttgac gctggtcatc accgagtaac gatctgccgg gcttttaccc
    17461 tgcatcgctt ccaccatggt tttgcccatt attgaggtga gatacgtatc ttcgccaaaa
    17521 ccttcggaag cacgtcccca gcgcggatcg cgcgagacat cgaccatcgg tgcccaggtc
    17581 atattcaggc catcatctgc cgcttcataa gcagagacac gcccgactgt tttcaccgca
    17641 tcgaggttaa aagacgaagc cagaccgaga ctaatcggga acaccgtgcg ctgaccgtgc
    17701 agcacgtcgt aagcaaagaa aagaggaatt ttcaggcggc ttaattccat cacctgatcc
    17761 tgcatggcgc ggatatcctg acgggttacg gtgttgaaaa tcgccccaac ctgaccgtct
    17821 ttgatcatct cgcggatcgc ctctttcggg ttatccgggc cgacgctgat taagcgcagc
    17881 tgaccaattt tctcatcaac tgtcattttc ttaagcagtt cggtgacgaa cgcatcccgc
    17941 gcttcgggcg ttaatggatg gttgccgaat aaatcatccg ccagtgctgg ctgcagggcc
    18001 agactcaccg cgattcctac tgaacatagc catttcatat ggatttttct tcctttgttg
    18061 cccgacgtgg cagcgaaaat ggtgcaaaaa ccgtagtttg ccataagcat gatggagaga
    18121 gaaaaagaat gctcagttta ttgtctgaat tttcaaaata ttcactcgct gaattgtcat
    18181 acaaggcgct attctagttt gtgatatttt ttcgccacca caaggagtgg aaaatgtctt
    18241 ccatgacaac aactgataat aaagcctttt tgaatgaact tgcccgtctg gtcggtcatt
    18301 cacacctgct caccgatccc gccaaaacgg cccgctatcg caagggcttc cgttctggtc
    18361 agggcgacgc gctggctgtc gttttccctg gctcactact ggaattgtgg cgggtgctga
    18421 aagcctgcgt caccgctgac aaaattattc tgatgcaggc tgccaataca ggcctgaccg
    18481 aaggatcgac gccaaacggt aacgattatg atcgcgatat cgtgatcatc agcaccctgc
    18541 gtctcgataa gctgcacgtt ctcggcaagg gcgaacaagt gctggcctat ccgggcacca
    18601 cgctctattc actggaaaaa gccctcaaac cgctgggacg cgaaccgcac tcagtgattg
    18661 gatcatcgtg tataggcgca tcggtcatcg gcggtatttg taacaactcc ggcggctcgc
    18721 tggtgcaacg tggcccggcg tataccgaaa tgtcgttatt cgcgcgtata aatgaagacg
    18781 gcaaactgac gctggtgaac catctgggga ttgatctggg cgaaacgccg gagcagatcc
    18841 ttagcaagct ggatgacgat cgcatcaaag atgacgatgt gcgccacgat ggtcgtcacg
    18901 cccacgatta tgactatgtc caccgcgttc gtgatattga agccaacacg cccgcacgtt
    18961 ataacgctga tccggatcgg ttatttgaat cttctggttg cgccgggaag ctggccgtct
    19021 ttgcggtacg tcttgatacc ttcgaagcgg aaaaaaatca gcaggtgttt tatatcggca
    19081 ccaaccagcc ggaagtgctg accgaaatcc gccgtcatat tctggctaat ttcgaaaatc
    19141 tgccggttgc cggggaatat atgcaccggg atatctacga tattgcggaa aaatacggca
    19201 aagacacctt cctgatgatt gataagttag gcaccgacaa gatgccgttc ttctttaatc
    19261 tcaagggacg caccgatgcg atgctggaga aagtgaaatt cttccgtccg cattttaccg
    19321 accgtgcaat gcaaaaattc ggtcacctgt tccccagcca tttaccgccg cgcatgaaaa
    19381 actggcgcga taaatacgag catcatctgc tgttaaaaat ggcgggcgat ggcgtcggtg
    19441 aagccaaatc gtggctagtg gattatttca aacaggccga gggcgatttc tttgtctgta
    19501 cgccggagga aggcagcaaa gcgtttttac accgtttcgc cgctgcgggc gcagcaattc
    19561 gttatcaggc tgtgcattcc gatgaagtcg aagacattct ggcgctggat atcgctctgc
    19621 ggcgtaacga caccgaatgg tatgagcatt taccgccgga gatcgacagc cagctggtgc
    19681 acaagctcta ttacggccat tttatgtgct atgtcttcca tcaggattac atcgtgaaaa
    19741 aaggcgtgga tgtgcatgcg ctgaaagaac agatgctgga actgcttcag cagcgtggcg
    19801 cgcaataccc tgccgagcat aacgtcggtc atttgtataa agcaccggaa acgttgcaga
    19861 agttttatcg cgagaacgat ccgaccaaca gtatgaatcc ggggattggt aaaaccagta
    19921 agcggaaaaa ctggcaggaa gtgaaataaa aattacggat ggcagagtat cgccatccgt
    19981 acttcattta atcgttctgt gccgtctgcc ccgccgccgc catttgggcg gctttttgtt
    20041 ttttatagct caacgctgct gccggaacag gcatcacttt accggtttca atccaggtac
    20101 gcaggcggct ggcatcggca aaatgggtat atttgccaaa cgcgtccatc actaccagcg
    20161 ccaccggttt attattgata accgtgcgca tcaccagaca atggcccgcc gcattggtaa
    20221 aaccggtttt ggttaactga atattccagt tatcacgata caccagatgg ttagtattgc
    20281 ggaacggcag cgtatacgtc gggttagaga aggttgccat atcttcccgg gtagtactta
    20341 actgcccgat caacggatat tgtttgctgg caatgagtag tttggttaag tcacgggcgg
    20401 ttgaaacgtt atgcaccgac aatccggttg gttcaacaaa gcgcgtgttg ttcattccga
    20461 gcgatttcgc tttcgcgttc atcgccttaa taaaggcttt gtaaccaccg ggataatggt
    20521 gcgcaagact tgccgccgcg cggttttctg aagacatcag cgccagcaac agcatatctt
    20581 tacggctgat ttcgctgttc agtcttacgc gcgaatagac ccctttcatc tccggcgtct
    20641 ggctgatatc cacttttagt ttttcatcca gcggcagtcg tgcatccagc acaaccatcg
    20701 cggtcattaa tttgctgata gacgcaatcg gacgcaccag atccgggtgg ttcgaataga
    20761 tcactttgtt ggtattcaga tcaacaatca tcgcgctacc ggaggcaatt tccggttgtg
    20821 acgcggtggt agcggctacc gttttcgcaa cggcctgcgg tgcaaaaggc acagccagca
    20881 tcagggccag gctaaataaa gaaactcgaa atttcggcat gatgagcatt cagatagtgg
    20941 ttcacgcgca cgggttgcgc accgccggag taaggattta ctgaggctag cgacgccatc
    21001 ataacgagca aaaagtgcga tcgtcaaaga agaatcgtga ggaaatgctg cattgctgac
    21061 atttacgcca gcaatgcagc gcaaaagaac tttctagaac aaacgataac cgtagcccca
    21121 tagtataacg gtaagggcga gcagcacttc cagcaccagc acgcctatcg ccagtgtcga
    21181 actggagaag ctaagtcctt cctctttgtt gatattcagg aagctcggaa taccgagata
    21241 cagcaggtag ccggtgtaaa acagcgccac cgtgccgacc agcgcacaca accagaccag
    21301 cgggtaaagc gccaccagac cgcttaaaaa cagcggagtt gcaacgtagc ccgcgaagac
    21361 catgcagtgc gcaagtgacg gacgctgcgg ataattacgc gccatccacc agatgacccg
    21421 ccccatcacc gcaaccccag ccagcataac gccgtaaaac aagacagcca gcgccagtcc
    21481 ggtaaaccag gataacttca ggatagtgcc atcgccaaaa ttccagccaa tctgtgtagt
    21541 gccaatgaag gcgcaaatca ccggaatcgc cgccatcagc aaaacgtggt gggtgtagtg
    21601 atgagaaatc gtttcgtttt cgcgattaat cacctgcatt tcacgatcgg gatgggaaaa
    21661 cagtccccag acatggctca taccgccccc ttgttgtgag ttcatgaacc tgacagttca
    21721 agtataagtc agcttgtgat tattttttgt tcgctcatgt aatttgcacc gtctgatgta
    21781 cgtccttttt cagcgtgaat gattcacagg gtgtatgctt acaggcaagc aaagggagac
    21841 agactgtcct atggatctca atacactcat ctcacaatat ggttatgccg cgctggtgat
    21901 cggtagcctg gcggaaggtg aaaccgtgac tttgctggga ggcgttgcgg cacatcaggg
    21961 gctattaaag ttcccgctgg tggtactttc tgtggcgctt ggcggcgtga ttggcgacca
    22021 ggtgctttat ctgtgcgggc ggcggtttgg cggcaagctg ttacgccgtt tctcgaaaca
    22081 tcaggataaa attgagcggg cgcagaaact tatccaacgc catccgtatc tgtttgtcat
    22141 tggtacgcgt tttatgtatg gctttcgggt cattggcccg acgctgattg gtgccagcca
    22201 gctgccaccg aaaatctttc tgccgctgaa tattctcggc gcatttgcct ggtcgctgat
    22261 ttttaccact atcggttacg ctggtgggca ggtgattgcg ccgtggttgc acaatctcga
    22321 ccagcatttg aagcactggg tctggttgat tctggtcgtg gttctggtgg taggcgtgcg
    22381 ctggtggctg aaacggcgcg ggaagaaaaa gccggatcat caggcgtaaa actattgccc
    22441 tggataaggt gttcgcgccg catccgacat ccggtgcacc atgcctgatg cgacgctggc
    22501 gtgtcttttc tggcctaacc caatgaaata accacgcacc tcactgcgct attctgggtt
    22561 gaactgcgga ttcgccaaca taaagccgcc atccactatc aacgactgcc cggtggtgta
    22621 atttgcgccc tccgaacaaa gccataccac caggctggca atctcatgcg ttgtgccaaa
    22681 acgtcgcaag ggaatcgaag gctccgcgtc aggcttcacg tcgccgccat ccatgccgtt
    22741 cattggcgtg gcgatcgccc caggtgcgac tgcgttcacc aaaattttat gcctgaccaa
    22801 ctccagcgcc attgctttgg ttaacccgcc gagcgcatgt ttagcggctg tgtaggcgct
    22861 ggcatccggc agcggcgtat gttcatgtac cgatgtaatg ttgatgatgc gaccgccctg
    22921 cccttgcttc accatctggc gggcagcaat ttgcgagcat aaaaatgcac catcgacatc
    22981 aacggtaaaa atcttgcgcc actcatcgaa agccatatca agaaacggcg ctttggtcat
    23041 tgcgcccgca ttattcacca gcacatcaat acgccccagc cgttgaataa gtttctccag
    23101 cgcctgcgca ccttctggta gattgccgag atccagttgc acgatctccg cacgtacgcc
    23161 gtggctaact acctcacgcg cggtatcttt tgccccttct tcatctgagt gccaggtaat
    23221 gccaatatca aacccctgct gcgccagtaa taacgcgcac tctttgccga tccccgaatc
    23281 ggaggcggta attatcgcga cctgtgccat cgagttctcc acttaacgct caacaaacgt
    23341 taagtataga aggcgcatat catcagcgtt tgtacccccc gcccagcgca ccagtgagtt
    23401 gaatggaggc atccagccac tgcccttgca ataacaggcc attggcccgc tcacgcagcg
    23461 ccgggattct ggcttcgctg acccgggaac cagcaatgag gcccgcgtta aagcgcgcct
    23521 gcgcaagacc tactacacgc aaggcatcgc gctcaatttg cgcctgatgc tggtttttct
    23581 ccgccagtgt ctgaacctga ctggctgccc gcgccacgtc attcaccgct tcaaccaccg
    23641 ctttgttgta gctggcgata gacaagttgc tttcggcttt tgcgatatcg agattggcgt
    23701 taagacgacc actatcgaaa atgggtagcg tcaggcctgc cgtaacgccc atttgctgcg
    23761 cggaatgacg gaacagatcg cttaagtgca acgcatcctg ttgcagaaag gccatcaggt
    23821 tgatgtcagg ataaaatgcc gcttttgccg catcaatagt gcttaacgat gactcaacgt
    23881 accagtgcgc cgcctgcaaa tctgcccgcc gggccagtaa ggagtacccc agttcatcag
    23941 gaagctgact tgccactttc ggcaacgcga ccggtttaag cttcaatgac tttgtctggt
    24001 ggttggtgag cgcacttaac cgtgcctcaa taattttcat tttccccgcg acatcgttga
    24061 gctgctgccg ggttttgctg gcattaatat cggtttccac accttcaact gcagaagtaa
    24121 tcccgttctg atatagctgg cgatcggtgg cgataatggt gttctgctct ttttcgattt
    24181 gctgtaagac cgtgtttaac gccgcctggg tttgccactc ccagtacagg cgggctacgc
    24241 tgccagccag caattggcgg gtttgctcgc gttccgccgc ccgtgcttta accgtaccga
    24301 ggcgagcagt aacctccgcc cgattctttc cccagatatc gagatgccag cccgccgtta
    24361 agccaaaagt accgttggtg taccacggac cggtcgtacc tgcggccgga tcgttcaaag
    24421 caaacggccc cattaaccct tctgccgaca ttttttgccg ctccatatcc gcagaaaagt
    24481 cgagctgcgg accatcctga gtggcaattg ccttcgcctg ggcttcagcc agctgaatgc
    24541 gctgttcagc cacctgcata tccggtgcgc tctgtagtgc gttattaatt aaggaggtga
    24601 gttgattatc gtgatactcc agccaccatt ggctgtctgg ccaaccattt tttagcgccg
    24661 tgggtaatac ggtgtcaact tgtgcagcgg gcgtttgctg gcttaacgcc tggcgggttt
    24721 catgcatagg cgcacacccg gccagcatca gtaacagcga aaaacaggcg atggcgggat
    24781 aaaaggaatc acgattcatg ggggaataat caggtaagaa aaggtgcgcg gagattaccg
    24841 tgtgttgcga tatatttttt agtttcgcgt ggcaatacat cagtagcaat aaaacgacat
    24901 atccagaaaa atatacacta agtgaatgat atctttcgat ttatcttaat cgtttatgga
    24961 taacggcaaa gggcttcgtt ttttcctata cttattcagc actcacaaat aaaggaacgc
    25021 caatgaaaat tatactctgg gctgtattga ttattttcct gattgggcta ctggtggtga
    25081 ctggcgtatt taagatgata ttttaaaaat cagatatggc atataagttg tcaggtcagt
    25141 aactcgatat tttgtgtact tgatttgcaa acagctccgg tgttggtggc gtcaaaatcg
    25201 ctgaagcgtt ccccgaaggc cggggcagcc cacatggatg tgggctgagg gcgcgtctta
    25261 caaggacgtt acctcgcgcc cgacccggta gccgaagggg ataagctgaa ggtaccgcgc
    25321 agcggcgatt ttgttcgcca gagcccgggg gggcaggggg aggaggcgat tgtcctcccc
    25381 ctgcgcgctc cttgcgccag tggcaatatg tggcttagct catgaaagga gcgcaacaag
    25441 atgatgaagc tgaataagcg attcattgtc tcagataagc tgagattcca ctattatcgc
    25501 agcttatcga tatcaatggc ctgaatagcc cttgcaatat caggggaatt attcaacacc
    25561 cgaacatgct gaaataattc cgttgcttca tcgtattctt tacgcaaata actcaaccac
    25621 tgtttaatcc gcgcaacgtg atataacccg gtatcgccct gcttttccag acgggtatat
    25681 ttttgcagca aagcaaccac ctccggccac ggcattcgcg gttcgttata tttcaccaca
    25741 cggctcaggt tgggaatatt gagcgccccg cgaccaatca tcactgcgtc gcagccgctg
    25801 atcgccatgc attgttgcgc actctgccag tcccagattt caccgttggc aatcaccgga
    25861 atattaagcc gctggcgaat ctcgccaatc gcctgccagt caatatgttc cgcgcggtaa
    25921 ccctgctctt ttgtccgccc atgtactacc agctccgtag cgccagcctg ttgcaccgca
    25981 tcggcgattt caaatttctt ctcaccgctg tcccagccca gacgcacttt tacgctgaca
    26041 ggcaaatgcg ccggtacagc ttcgcgcatc gcttttgcac cctggtagat gagttcagga
    26101 tctttcagta acgttgcccc accaccgcta ccgttaaccg ttttcgacgg gcagccgcag
    26161 ttaagatcca cgccccagga acctaactcc accgcacggg cggcgttctc tgccagccac
    26221 tgtgggaact gccctaataa ctgcacgcga accagcgtac cggatggtgt ccggctggca
    26281 ttttgtagct cagggcaaat gcgatgaaag acttttactg gcagcagttg atccaccacg
    26341 cggacaaact cggtgatgca cagatcgtag tcgttaactt cggtcagtaa ttcccgcacc
    26401 aaagagtcaa gtacaccctc catcggagcc agtaacacac gcatatcatc acccgcaaaa
    26461 aaatgaggcg ctatgttagc gcctccggtc aacggattaa aggtctgcaa agagggattg
    26521 ctgcaaactg caatccctct ctatgcttga tgtatgcctg agttcgctgc gcattgcgta
    26581 ataccgggga gcgaaaaaat aagcgctgat ttcttaatgt gatcggtagc acgttttacg
    26641 aattaattgt atgatgaatc catctcatct ggggtgttga ttatgagtaa gacactgaac
    26701 attatctggc aatatttacg cgctttcgtc ctgatttatg cctgcctgta tgcaggcatt
    26761 ttcattgctt ccctgctacc ggtaaccatt ccgggcagca tcatcgggat gctgatcctg
    26821 tttgtcctgc tggccttgca aattcttccg gcgaaatggg tcaatccggg gtgctacgta
    26881 ctgattcgct atatggcgct attgtttgtg ccgattggcg taggcgtcat gcaatatttt
    26941 gatttgctcc gcgcacagtt tggtccggta gtggtttcct gtgcagtcag tacgctggtg
    27001 gtttttctgg tggtgagctg gagttcacaa ctggtgcacg gtgaacgtaa agtcgtaggt
    27061 cagaaaggat cagaagaatg atggcgaata tctggtggtc attaccgctg acgttgattg
    27121 tcttttttgc cgcccgcaaa ctggcggcac ggtataagtt tcccctgctt aacccgttgc
    27181 tggtagcaat ggtggtgatc attccttttt taatgctgac tggcatctct tacgacagct
    27241 actttaaagg tagcgaagtg ctaaacgatc tgctgcaacc ggcggtcgtc gcactggctt
    27301 atcctttata tgagcagcta caccagatcc gcgcgcgctg gaaatcgatc atcaccatct
    27361 gttttatcgg cagcgtggtt gcgatggtga cggggacttc cgtggcattg ttgatgggcg
    27421 cttcaccgga aatcgccgcg tcaatcctgc cgaaatcagt taccacgcct attgcaatgg
    27481 cggttggcgg cagtattggc ggtattccgg caatcagcgc agtttgcgtg attttcgtcg
    27541 gcatcctcgg cgctgtgttt ggccatacat tgcttaatgc gatgcgtatt cgtaccaaag
    27601 ctgcgcgcgg tctggcaatg ggcacagcct cgcacgccct cggtacggcg cgctgcgccg
    27661 agctggatta tcaggaaggt gcatttagtt cgctggcgct ggtgttatgc gggattatta
    27721 cttcgctgat cgcaccgttc cttttcccga ttattctggc agtaatgggc taaaatttgc
    27781 gatgcgtcgc gcatttttga tgtaagtttc acgcgttgca taattaatga gattcagatc
    27841 acatataaag ccataacggg ttcgtaaact gttatcccat tacatgatta tgaggcaacg
    27901 ccatgcatcc acgttttcaa accgcttttg cccaacttgc ggataacttg caatctgcac
    27961 tggaacctat tctggcagac aagtacttcc ccgctttgtt gaccggggag caagtctcat
    28021 cgctgaagag cgcaacgggg ctggacgaag acgcgctggc attcgcacta cttccgctgg
    28081 cggcagcctg tgcgcgtacg ccattgtcga attttaatgt tggcgcaatt gcgcgcggtg
    28141 tgagcggaac ctggtatttc ggtgccaata tggaatttat tggtgcgaca atgcagcaaa
    28201 ccgttcatgc cgaacaaagc gcgatcagcc acgcctggct gagtggtgaa aaagcgcttg
    28261 cagccatcac cgttaactac acgccttgtg gtcactgccg tcagtttatg aatgaactga
    28321 acagcggtct ggatctgcgt attcatcttc cgggacgcga ggcacacgcg ctgcgtgact
    28381 atctaccgga tgcctttggg ccgaaagatc tggagattaa aacgctgctg atggacgagc
    28441 aggatcacgg ctatgcgctg actggtgatg cgctttctca ggcggcgatt gcggcggcaa
    28501 accgttcgca catgccttac agtaagtcgc caagcggtgt cgcgctggaa tgtaaagacg
    28561 gtcgtatttt cagcggcagc tacgctgaaa acgccgcatt caacccgact ctgccgccgt
    28621 tgcagggggc gttaattctg ttgaatctca agggttatga ttacccggat atccagcgcg
    28681 cggttctggc agaaaaagcc gatgcgccgt tgattcagtg ggatgccacc tccgcaacgc
    28741 tgaaagctct cggctgtcac aatatcgacc gcgtgcttct cgcttaagcc tggtgccgga
    28801 tgcggcgtga acgccttgtc cggcttgcca gccctttcct ggtgtcgaaa atcccggcaa
    28861 acagtttgcc gtttcttgcg caaaaccagc gggtaaagta gcctgatgga aattttcctt
    28921 agatcgagtc tcctgcatgt taaagcgcgt gttcctcagc ctgttagtcc tgatcggctt
    28981 gctgctgttg actgtgctcg gcctcgatcg ctggatgagc tggaaaaccg cgccttatat
    29041 ctacgacgaa ttgcaggatc tcccctaccg ccaggtcggt gtggtgctcg gcacagcaaa
    29101 atattatcgg actggcgtaa ttaatcagta ttatcgctac cgcattcaag gagcgattaa
    29161 tgcctataac agcggtaagg taaattatct attactgagc ggcgataacg cattgcaaag
    29221 ttataatgag ccgatgacca tgcgcaaaga tttaatcgca gctggtgtcg acccatcaga
    29281 tattgttctc gattacgcag gctttcgtac gctggattcc atcgtgcgta cacgcaaagt
    29341 tttcgatact aatgatttca ttattatcac ccaacgtttc cactgtgagc gggcattatt
    29401 tattgcgctg catatgggga ttcaggctca gtgttatgcc gtaccgtcac cgaaagatat
    29461 gctgtcagta cgtattcgcg aatttgccgc ccgtttcggt gcgctggctg acctttatat
    29521 ttttaaacgt gaaccgcgtt ttttagggcc gctggtccct attccggcta tgcaccaggt
    29581 gccggaagat gcgcaggggt atcccgccgt cacacccgaa cagttacttg aattacaaaa
    29641 gaaacaagga aagtagttat ggatgtacag cagttttttg tcgttgccgt ttttttcctt
    29701 attccgatat tttgtttccg cgaagcatgg aaaggctggc gcgcaggcgc gattgataaa
    29761 cgggttaaaa atgcaccaga accggtgtat gtctggcgag cgaaaaatcc cggactcttc
    29821 ttcgcttata tggtggcata tatcggcttc ggaattttat ctatcggcat gattgtttat
    29881 cttattttct at
//
LOCUS       DADSYM010000045        29794 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2415
            SAMN08039579-rid11723173.denovo.045, whole genome shotgun sequence.
ACCESSION   DADSYM010000045 DADSYM010000000
VERSION     DADSYM010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039579
            Sequence Read Archive: SRR6362533
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 29794)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29794)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980162.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 03:39:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,484
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 4,347
            CDSs (with protein)               :: 4,347
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 43 of 85
            Pseudo Genes (incomplete)         :: 37 of 85
            Pseudo Genes (internal stop)      :: 19 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29794
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039579-rid11723173.denovo.045"
                     /strain="LGE2415"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8302 N 95.9628 W"
                     /collection_date="2015-08-24"
     gene            <1..144
                     /locus_tag="J1Y83_001702"
     CDS             <1..144
                     /locus_tag="J1Y83_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HBA7629853.1"
                     /translation="VEMVMPGDNIKMVVTLIHPIAMDDGLRFAIREGGRTVGAGVVAK
                     VLS"
     gene            374..757
                     /gene="secE"
                     /locus_tag="J1Y83_001703"
     CDS             374..757
                     /gene="secE"
                     /locus_tag="J1Y83_001703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007665874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HBA7629854.1"
                     /translation="MSANTEAQGSGRGLEAMKWVVVVALLLVAIVGNYLYRDIMLPLR
                     ALAVVILIAAAGGVALLTTKGKATVAFAREARTEVRKVIWPTRQETLHTTLIVAAVTA
                     VMSLILWGLDGILVRLVSFITGLRF"
     gene            759..1304
                     /gene="nusG"
                     /locus_tag="J1Y83_001704"
     CDS             759..1304
                     /gene="nusG"
                     /locus_tag="J1Y83_001704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005280494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HBA7629855.1"
                     /translation="MSEAPKKRWYVVQAFSGFEGRVATSLREHIKLHNMEELFGEVMV
                     PTEEVVEIRGGQRRKSERKFFPGYVLVQMVMNDASWHLVRSVPRVMGFIGGTSDRPAP
                     ISDKEVDAIMNRLQQVGDKPRPKTLFEPGEMVRVNDGPFADFNGVVEEVDYEKSRLKV
                     SVSIFGRATPVELDFSQVEKA"
     gene            1463..1891
                     /gene="rplK"
                     /locus_tag="J1Y83_001705"
     CDS             1463..1891
                     /gene="rplK"
                     /locus_tag="J1Y83_001705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006121495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HBA7629856.1"
                     /translation="MAKKVQAYVKLQVAAGMANPSPPVGPALGQQGVNIMEFCKAFNA
                     KTDSIEKGLPIPVVITVYADRSFTFVTKTPPAAVLLKKAAGIKSGSGKPNKDKVGKIS
                     RAQLQEIAQTKAADMTGADIEAMTRSIEGTARSMGLVVED"
     gene            1895..2599
                     /gene="rplA"
                     /locus_tag="J1Y83_001706"
     CDS             1895..2599
                     /gene="rplA"
                     /locus_tag="J1Y83_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HBA7629857.1"
                     /translation="MAKLTKRMRVIREKVDATKQYDINEAIALLKELATAKFVESVDV
                     AVNLGIDARKSDQNVRGATVLPHGTGRSVRVAVFTQGANAEAAKAAGAELVGMEDLAE
                     QIKKGEMNFDVVIASPDAMRVVGQLGQVLGPRGLMPNPKVGTVTPNVAEAVKNAKAGQ
                     IRYRNDKNGIIHTTIGKVDFDADKLKENLEALLVALKKAKPTQAKGVYIKKVSISTTM
                     GAGVAVDQAGLSASVN"
     gene            2891..3388
                     /gene="rplJ"
                     /locus_tag="J1Y83_001707"
     CDS             2891..3388
                     /gene="rplJ"
                     /locus_tag="J1Y83_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002438625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HBA7629858.1"
                     /translation="MALNLQDKQAIVAEVSEVAKGALSAVVADSRGVTVDKMTELRKA
                     GREAGVYMRVVRNTLLRRAVEGTPFECLKDAFVGPTLIAYSMEHPGAAARLFKEFAKA
                     NAKFEVKAAAFEGELIPASQIDRLATLPTYEEAIARLMATMKEASAGKLVRTLAAVRD
                     AKEAA"
     gene            3455..3820
                     /gene="rplL"
                     /locus_tag="J1Y83_001708"
     CDS             3455..3820
                     /gene="rplL"
                     /locus_tag="J1Y83_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HBA7629859.1"
                     /translation="MSITKDQIIEAVAAMSVMDVVELISAMEEKFGVSAAAAVAVAAG
                     PVEAAEEKTEFDVILKAAGANKVAVIKAVRGATGLGLKEAKDLVESAPAALKEGVSKD
                     DAEALKKALEEAGAEVEVK"
     gene            4140..8168
                     /gene="rpoB"
                     /locus_tag="J1Y83_001709"
     CDS             4140..8168
                     /gene="rpoB"
                     /locus_tag="J1Y83_001709"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HBA7629860.1"
                     /translation="MVYSYTEKKRIRKDFGKRPQVLDVPYLLSIQLDSFQKFIEQDPE
                     GQYGLEAAFRSVFPIQSYSGNSELQYVSYRLGEPVFDVQECQIRGVTYSAPLRVKLRL
                     VIYEREAPEGTVKDIKEQEVYMGEIPLMTDNGTFVINGTERVIVSQLHRSPGVFFDSD
                     KGKTHSSGKVLYNARIIPYRGSWLDFEFDPKDNLFVRIDRRRKLPATIILRALNYTTE
                     QILDLFFEKVIFEIRDNKLQMELVPERLRGETASFDIEANGKVYVEKGRRITARHIRQ
                     LEKDDVKLIEVPVEYIAGKVVAKDYIDESTGELICAANMELSLDLLAKLSQSGHKRIE
                     TLFTNDLDHGPYISETLRVDPTNDRLSALVEIYRMMRPGEPPTREAAESLFENLFFSE
                     DRYDLSAVGRMKFNRSLLREEIEGSGILSKDDIIDVMKKLIDIRNGKGEVDDIDHLGN
                     RRIRSVGEMAENQFRVGLVRVERAVKERLSLGDLDTLMPQDMINAKPISAAVKEFFGS
                     SQLSQFMDQNNPLSEITHKRRISALGPGGLTRERAGFEVRDVHPTHYGRVCPIETPEG
                     PNIGLINSLSVYAQTNEYGFLETPYRKVTDGVVTDEIHYLSAIEEGNYVIAQANSNLD
                     EEGHFVEDLVTCRSKGESSLFSRDQVDYMDVSTQQVVSVGASLIPFLEHDDANRALMG
                     ANMQRQAVPTLRADKPLVGTGMERAVAVDSGVTAVAKRGGVVQYVDASRIVIKVNEDE
                     MYPGEAGIDIYNLTKYTRSNQNTCINQMPCVSLGEPVERGDVLADGPSTDLGELALGQ
                     NMRVAFMPWNGYNFEDSILVSERVVQEDRFTTIHIQELACVSRDTKLGPEEITADIPN
                     VGEAALSKLDESGIVYIGAEVTGGDILVGKVTPKGETQLTPEEKLLRAIFGEKASDVK
                     DSSLRVPNGVSGTVIDVQVFTRDGVEKDKRALEIEEMQLKQAKKDLSEELQILEAGLF
                     SRIRAVLVAGGVEAEKLDKLPRDRWLELGLTDEEKQNQLEQLAEQYDELKHEFEKKLE
                     AKRRKITQGDDLAPGVLKIVKVYLAVKRRIQPGDKMAGRHGNKGVISKINPIEDMPYD
                     ENGTPVDIVLNPLGVPSRMNIGQILETHLGMAAKGIGDKINAMLKQQQEVAKLREFIQ
                     RAYDLGADVRQKVDLSTFSDEEVMRLAENLRKGMPIATPVFDGAKEAEIKELLKLGDL
                     PTSGQIRLYDGRTGEQFERPVTVGYMYMLKLNHLVDDKMHARSTGSYSLVTQQPLGGK
                     AQFGGQRFGEMEVWALEAYGAAYTLQEMLTVKSDDVNGRTKMYKNIVDGNHQMEPGMP
                     ESFNVLLKEIRSLGINIELEDE"
     gene            8245..12468
                     /gene="rpoC"
                     /locus_tag="J1Y83_001710"
     CDS             8245..12468
                     /gene="rpoC"
                     /locus_tag="J1Y83_001710"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HBA7629861.1"
                     /translation="MKDLLKFLKAQTKTEEFDAIKIALASPDMIRSWSFGEVKKPETI
                     NYRTFKPERDGLFCARIFGPVKDYECLCGKYKRLKHRGVICEKCGVEVTQTKVRRERM
                     GHIELASPTAHIWFLKSLPSRIGLLLDMPLRDIERVLYFESYVVIEGGMTNLERQQIL
                     TEEQYLDALEEFGDEFDAKMGAEAIQALLKSMDLEQECEQLREELNETNSETKRKKLT
                     KRIKLLEAFVQSGNKPEWMILTVLPVLPPDLRPLVPLDGGRFATSDLNDLYRRVINRN
                     NRLKRLLDLAAPDIIVRNEKRMLQEAVDALLDNGRRGRAITGSNKRPLKSLADMIKGK
                     QGRFRQNLLGKRVDYSGRSVITVGPYLRLHQCGLPKKMALELFKPFIYGKLELRGLAT
                     TIKAAKKMVEREEAVVWDILDEVIREHPVLLNRAPTLHRLGIQAFEPVLIEGKAIQLH
                     PLVCAAYNADFDGDQMAVHVPLTLEAQLEARALMMSTNNILSPANGEPIIVPSQDVVL
                     GLYYMTRDCVNAKGEGMVLTGPKEAERLYRSGLASLHARVKVRITEYEKDANGELVAK
                     TSLKDTTVGRAILWMIVPKGLPYTIVNQALGKKAISKMLNTCYRILGLKPTVIFADQI
                     MYTGFAYAARSGASVGIDDMVIPEKKHEIISEAEAEVAEIQEQFQSGLVTAGERYNKV
                     IDIWAAANDRVSKAMMDNLQTETVINRDGQEEKQVSFNSIYMMADSGARGSAAQIRQL
                     AGMRGLMAKPDGSIIETPITANFREGLNVLQYFISTHGARKGLADTALKTANSGYLTR
                     RLVDVAQDLVVTEDDCGTHEGIMMTPVIEGGDVKEPLRDRVLGRVTAEDVLKPGTADI
                     LVPRNTLLHEQWCDLLEENSVDAVKVRSVVSCDTDFGVCAHCYGRDLARGHIINKGEA
                     IGVIAAQSIGEPGTQLTMRTFHIGGAASRAAAESSIQVKNKGSIKLSNVKSVVNSSGK
                     LVITSRNTELKLIDEFGRTKESYKVPYGAVLAKGDGEQVAGGETVANWDPHTMPVITE
                     VSGFVRFTDMIDGQTITRQTDELTGLSSLVVLDSAERTAGGKDLRPALKIVDAQGNDV
                     LIPGTDMPAQYFLPGKAIVQLEDGVQISSGDTLARIPQESGGTKDITGGLPRVADLFE
                     ARRPKEPAILAEISGIVSFGKETKGKRRLVITPVDGSDPYEEMIPKWRQLNVFEGERV
                     ERGDVISDGPEAPHDILRLRGVHAVTRYIVNEVQDVYRLQGVKINDKHIEVIVRQMLR
                     KATIVNAGSSDFLEGEQVEYSRVKIANRELEANGKVGATYSRDLLGITKASLATESFI
                     SAASFQETTRVLTEAAVAGKRDELRGLKENVIVGRLIPAGTGYAYHQDRMRRRAAGEA
                     PAAPQVTAEDASASLAELLNAGLGGSDNE"
     gene            complement(12709..13842)
                     /gene="thiH"
                     /locus_tag="J1Y83_001711"
     CDS             complement(12709..13842)
                     /gene="thiH"
                     /locus_tag="J1Y83_001711"
                     /EC_number="4.1.99.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000847471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminoacetate synthase ThiH"
                     /protein_id="HBA7629862.1"
                     /translation="MKTFSDRWRQLDWDDIHLRINGKTAADVERALNASQLTRDDMMA
                     LLSPAASGYLEPLAQRAQRLTRQRFGNVVSFYVPLYLSNLCANDCTYCGFSMSNRIKR
                     KTLDEADIARESAAIREMGFEHLLLVTGEHQAKVGMDYFRRHLPALREQFSSLQMEVQ
                     PLAEAEYAELKQLGLDGVMVYQETYNEATYAHHHLKGKKQDFFWRLETPDRLGRAGID
                     KIGLGALIGLSDSWRVDCYMVAEHLLWLQQHYWQSRYSVSFPRLRPCTGGIEPASIMD
                     ERQLVQAICAFRLLAPEIELSLSTRESPWFRDRVIPLAINNVSAFSKTQPGGYADNHP
                     ELEQFSPHDDRRPEAVAAALTAQGLQPVWKDWDSYLGRPSQRP"
     gene            complement(13839..14609)
                     /gene="thiG"
                     /locus_tag="J1Y83_001712"
     CDS             complement(13839..14609)
                     /gene="thiG"
                     /locus_tag="J1Y83_001712"
                     /EC_number="2.8.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418418.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HBA7629863.1"
                     /translation="MLHIADKTFDSHLFTGTGKFASSQLMVESIRASGSQLVTLAMKR
                     VDLRQHNDAILEPLIAAGVTLLPNTSGAKTAEEAIFAAHLAREALGTNWLKLEIHPDA
                     RWLLPDPIETLKAAETLVQQGFIVLPYCGADPVLCKRLEEVGCAAVMPLGAPIGSNQG
                     LETRAMLEIIIQQATVPVVVDAGIGVPSHAAQALEMGADAVLVNTAIAVVDDPVNMAK
                     AFRLAVEAGLLARQSGPGSRSHFAHATSPLTGFLEASA"
     gene            complement(14611..14811)
                     /gene="thiS"
                     /locus_tag="J1Y83_001713"
     CDS             complement(14611..14811)
                     /gene="thiS"
                     /locus_tag="J1Y83_001713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HBA7629864.1"
                     /translation="MQILFNDQPMQCVAGLTVHELLEQLDQQQAGAALAINQQIVPRE
                     QWAQHIVQDGDQILLFQVIAGG"
     gene            complement(14795..15550)
                     /gene="thiF"
                     /locus_tag="J1Y83_001714"
     CDS             complement(14795..15550)
                     /gene="thiF"
                     /locus_tag="J1Y83_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418420.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole biosynthesis adenylyltransferase ThiF"
                     /protein_id="HBA7629865.1"
                     /translation="MNDRDFMRYSRQILLDDIALDGQQKLLDSQVLIIGLGGLGTPAA
                     LYLAGAGVGTLVLADDDDVHLSNLQRQILFTTEDIDRPKSQVSQQRLTQLNPDIQLMA
                     LQQRLTGETLKDAVAQADVVLDCTDNMATRQEINATCVALNTPLITASAVGFGGQLMV
                     LTPPWEQGCYRCLWPDNQEPERNCRTAGVVGPVVGVMGTLQALEAIKLLSGIETPAGE
                     LRLFDGKSSQWRSLTLRRANGCPVCGGCNADPV"
     gene            complement(15543..16178)
                     /gene="thiE"
                     /locus_tag="J1Y83_001715"
     CDS             complement(15543..16178)
                     /gene="thiE"
                     /locus_tag="J1Y83_001715"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HBA7629866.1"
                     /translation="MYQPEFPPVPFRLGLYPVVDSVQWIERLLDAGVRTLQLRIKDRR
                     DEEVEADVVAAIALGRRYNARLFINDYWRLAIKHQAYGVHLGQEDLQATDLSAIRAAG
                     LRLGVSTHDDMEIDVALAARPSYIALGHVFPTQTKQMPSAPQGLEQLARHVERLADYP
                     TVAIGGISLARAPAVIATGVGSIAVVSAITQAVDWRLATAQLLEIAGVGDE"
     gene            complement(16178..18073)
                     /gene="thiC"
                     /locus_tag="J1Y83_001716"
     CDS             complement(16178..18073)
                     /gene="thiC"
                     /locus_tag="J1Y83_001716"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HBA7629867.1"
                     /translation="MSATKLTRREQRAQAQHFIDTLEGSAFPNSKRIYITGTHPGVRV
                     PMREIQLSPTLIGGSKEQPQYEENEAIPVYDTSGPYGDPQIAINVQQGLAKLRQPWID
                     ARGDTEELTVRSSDYTKARLADDGLDELRFSGVLTPKRAKAGRRVTQLHYARQGIITP
                     EMEFIAIRENMGRERIRSEVLRHQHPGMSFGARLPENITAEFVRDEVAAGRAIIPANI
                     NHPESEPMIIGRNFLVKVNANIGNSAVTSSIEEEVEKLVWSTRWGADTVMDLSTGRYI
                     HETREWILRNSPVPIGTVPIYQALEKVNGIAEDLTWEVFRDTLLEQAEQGVDYFTIHA
                     GVLLRYVPMTAKRLTGIVSRGGSIMAKWCLSHHQENFLYQHFREICEICAAYDVSLSL
                     GDGLRPGSIQDANDEAQFAELHTLGELTKIAWEYDVQVMIEGPGHVPMQMIRRNMTEE
                     LEHCHEAPFYTLGPLTTDIAPGYDHFTSGIGAAMIGWFGCAMLCYVTPKEHLGLPNKE
                     DVKQGLITYKIAAHAADLAKGHPGAQIRDNAMSKARFEFRWEDQFNLALDPFTARAYH
                     DETLPQESGKVAHFCSMCGPKFCSMKISQEVRDYAAAQTIEVGMADMSENFRARGGEI
                     YLRKEEA"
     regulatory      complement(18154..18254)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(18306..18782)
                     /locus_tag="J1Y83_001717"
     CDS             complement(18306..18782)
                     /locus_tag="J1Y83_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rsd/AlgQ family anti-sigma factor"
                     /protein_id="HBA7629868.1"
                     /translation="MLNQLDNLTERVRGSNKLVDRWLHVRKHLLVAYYNLVGIKPGKE
                     SYMRLNEKALDDFCQSLVDYLSAGHFSIYERILHKLEGNGQLARAAKIWPQLEANTQQ
                     IMDDYDSSLETAIDHDNYLEFQQVLSDIGEALEARFVLEDKLILLVLDAALVKHPA"
     gene            18877..19650
                     /gene="nudC"
                     /locus_tag="J1Y83_001718"
     CDS             18877..19650
                     /gene="nudC"
                     /locus_tag="J1Y83_001718"
                     /EC_number="3.6.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) diphosphatase"
                     /protein_id="HBA7629869.1"
                     /translation="MDRIIEKLDHGWWVVSHEQKLWLPKGELPYGEAANFDLVGQRAL
                     QIGEWQGEPVWLVQLQRRHDMGSVRQVIDLDVGLFQLAGRGVQLAEFYRSHKYCGYCG
                     HEMYPSKTEWAMLCSHCRERYYPQIAPCIIVAIRRDDSILLAQHTRHRNGVHTVLAGF
                     VEVGETLEQAVAREVMEESGIKVKNLRYVTSQPWPFPQSLMTAFMAEYDSGEIVIDPK
                     ELLEANWYRYDDLPLLPPPGTVARRLIEDTVAMCRAEYE"
     gene            19690..20754
                     /gene="hemE"
                     /locus_tag="J1Y83_001719"
     CDS             19690..20754
                     /gene="hemE"
                     /locus_tag="J1Y83_001719"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HBA7629870.1"
                     /translation="MTELKNDRYLRALLRQPVDVTPVWMMRQAGRYLPEYKATRAQAG
                     DFMSLCKNAELACEVTLQPLRRYPLDAAILFSDILTVPDAMGLGLYFEAGEGPRFTSP
                     VTCKADVDKLPIPDPEDELGYVMNAVRTIRRELKGEVPLIGFSGSPWTLATYMVEGGS
                     SKAFTVIKKMMYADPQALHALLDKLAKSVTLYLNAQIKAGAQAVMIFDTWGGVLTGRD
                     YQQFSLYYMHKIVDGLLRENDGRRVPVTLFTKGGGQWLEAMAETGCDALGLDWTTDIA
                     DARRRVGNKVALQGNMDPSMLYAPPARIEEEVASILAGFGHGEGHVFNLGHGIHQDVP
                     PEHAGVFVEAVHRLSEQYHR"
     gene            20764..21435
                     /gene="nfi"
                     /locus_tag="J1Y83_001720"
     CDS             20764..21435
                     /gene="nfi"
                     /locus_tag="J1Y83_001720"
                     /EC_number="3.1.21.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease V"
                     /protein_id="HBA7629871.1"
                     /translation="MDLASLRAQQIELASSVIREDRLDKDPPDLIAGADVGFEQGGEV
                     TRAAMVLLKYPSLELVEYKVARIATTMPYIPGFLSFREYPALLAAWEMLSQKPDLVFV
                     DGHGISHPRRLGVASHFGLLVDVPTIGVAKKRLCGKFEPLSSEPGALAPLMDKGEQLA
                     WVWRSKARCNPLFIATGHRVSVDSALAWVQRCMKGYRLPEPTRWADAVASERPAFVRY
                     TANQP"
     gene            21478..22068
                     /locus_tag="J1Y83_001721"
     CDS             21478..22068
                     /locus_tag="J1Y83_001721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjaG family protein"
                     /protein_id="HBA7629872.1"
                     /translation="MLQNPIHLRLERLESWQHVTFMACLCERMYPNYAVFCQQTGFGD
                     GQIYRRILDLIWETLTVKDAKVNFDSQLEKFEEAIPSADDFDLYGVYPAIDACVALSE
                     LVHSRLSGETLEHAVEVSKTSITTVAMLEMTQAGREMSDEELKENPAVEQEWDIQWEI
                     FRLLAECEERDIELIKGLRADLREAGESNIGIIFQQ"
     gene            22255..22527
                     /gene="hupA"
                     /locus_tag="J1Y83_001722"
     CDS             22255..22527
                     /gene="hupA"
                     /locus_tag="J1Y83_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017460010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein HU-alpha"
                     /protein_id="HBA7629873.1"
                     /translation="MNKTQLIDVIAEKAELSKTQAKAALESTLAAITESLKEGDAVQL
                     VGFGTFKVNHRAERTGRNPQTGKEIKIAAANVPAFVSGKALKDAVK"
     gene            22540..23235
                     /locus_tag="J1Y83_001723"
     CDS             22540..23235
                     /locus_tag="J1Y83_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709795.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1481 domain-containing protein"
                     /protein_id="HBA7629874.1"
                     /translation="MNSFNEGVVSPLLSFWRRSLMLAGALFLTACSHNSSLPPFTASG
                     FAEDQGAVRIWRKDSGDNVHLLAVFSPWRSGDTTTREYRWQGDNLTLININVYSKPPV
                     NIRARFDDRGDLSFMQRESDGEKQQLSNDQIDLYRYRADQIRQISDALRQGRVVLRQG
                     RWHAMEQTVTTCEGQTIKPDLDSQAIAHIERRQSRSSVDVSVAWLEAPEGSQLLLVAN
                     SDFCRWQPNEKTF"
     gene            complement(23237..23662)
                     /gene="zraP"
                     /locus_tag="J1Y83_001724"
     CDS             complement(23237..23662)
                     /gene="zraP"
                     /locus_tag="J1Y83_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc resistance sensor/chaperone ZraP"
                     /protein_id="HBA7629875.1"
                     /translation="MKRNTKIALVMMALSAMAMGSTSAFAHGGHGMWQQNAAPLTSEQ
                     QTAWQKIHNDFYAQSSALQQQLVTKRYEYNALLAANPPDSSKINAVAKEMENLRQSLD
                     ELRVKRDIAMAEAGIPRGAGMGMGYGGCGGGGHMGVGHW"
     gene            23900..25276
                     /gene="zraS"
                     /locus_tag="J1Y83_001725"
     CDS             23900..25276
                     /gene="zraS"
                     /locus_tag="J1Y83_001725"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     ZraS"
                     /protein_id="HBA7629876.1"
                     /translation="MRFMQRSKDSLAKWLSAILPMVIVGLVGLFAVTVIRDYGRETAA
                     ARQTLLEKGSVLIRALESGSRVGMGMRMHHAQQQALLEEMAGQPGVRWFAVTDEQGTI
                     VMHSNSGMMGKQLYSPQEMQQLHPGDEEAWRRIDSADGEPVLEIYRQFQPMFAAGMHR
                     MRHMQQYAATPQAIFIAFDASNIVSAEDREQRNTLIILFALATVLLASVLSFFWYRRY
                     LRSRQLLQDEMKRKEKLVALGHLAAGVAHEIRNPLSSIKGLAKYFAERAPAGGEAHQL
                     AQVMAKEADRLNRVVSELLELVKPTHLALQAVELNTLISHSLQLVSQDANSREIQLRF
                     TANDTLPEIQADPDRLTQVLLNLYLNAIQAIGQHGVISVTASESGAGVKISVTDSGKG
                     IAADQLEAIFTPYFTTKAEGTGLGLAVVHNIVEQHGGTIQVASQEGKGATFTLWLPVN
                     ITRKDPQG"
     gene            25273..26598
                     /gene="zraR"
                     /locus_tag="J1Y83_001726"
     CDS             25273..26598
                     /gene="zraR"
                     /locus_tag="J1Y83_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent response regulator
                     transcription factor ZraR"
                     /protein_id="HBA7629877.1"
                     /translation="MTHDNIDILVVDDDISHCTILQALLRGWGYNVALANSGRQALEQ
                     VREQVFDLVLCDVRMAEMDGIATLKEIKTLNPAIPVLIMTAYSSVETAVEALKTGALD
                     YLIKPLDFDNLQATLEKALAHTHSVDAETPAVSASQFGMVGKSPAMQHLLSEIALVAP
                     SEATVLIHGDSGTGKELVARAIHASSARSEKSLVTLNCAALNESLLESELFGHEKGAF
                     TGADKRREGRFVEADGGTLFLDEIGDISPMMQVRLLRAIQEREVQRVGSNQTISVDVR
                     LIAATHRDLAAEVNAGRFRQDLYYRLNVVAIEVPSLRQRREDIPLLANHFLQRFAERN
                     RKAVKGFTPQAMDLLIHYDWPGNIRELENAVERAVVLLTGEYISERELPLAIASTPIP
                     LVQSQDIQPLVEVEKEVILAALEKTGGNKTEAARQLGITRKTLLAKLSR"
     gene            complement(26595..27884)
                     /gene="purD"
                     /locus_tag="J1Y83_001727"
     CDS             complement(26595..27884)
                     /gene="purD"
                     /locus_tag="J1Y83_001727"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HBA7629878.1"
                     /translation="MKVLVIGNGGREHALAWKAAQSPLVETVFVAPGNAGTALEPALQ
                     NVAIGVTDIPALLDFAQNEKVDLTIVGPEAPLVKGVVDTFRAAGLKIFGPTAGAAQLE
                     GSKAFTKDFLARHNIPTAEYQNFTEVEPALAYLREKGAPIVIKADGLAAGKGVIVAMT
                     LEEAEAAVRDMLAGNAFGDAGHRIVIEEFLDGEEASFIVMVDGEHVLPMATSQDHKRV
                     GDKDTGPNTGGMGAYSPAPVVTDEVHQRTMERIIWPTVKGMAAEGNTYTGFLYAGLMI
                     DKQGNPKVIEFNCRFGDPETQPIMLRMKSDLVELCLAACEGKLDEKTSEWDERASLGV
                     VMAAGGYPGDYRTGDVIHGLPLEEVADGKVFHAGTKLADDEQVVTSGGRVLCVTALGH
                     TVAEAQKRAYALMTDIHWDDCFCRKDIGWRAIEREQN"
     gene            complement(27896..29485)
                     /gene="purH"
                     /locus_tag="J1Y83_001728"
     CDS             complement(27896..29485)
                     /gene="purH"
                     /locus_tag="J1Y83_001728"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HBA7629879.1"
                     /translation="MQQRRPVRRALLSVSDKAGIVEFAQALSARGVELLSTGGTARLL
                     AEKGLPVTEVSDYTGFPEMMDGRVKTLHPKVHGGILGRRGQDDAIMEEHQIQPIDMVV
                     VNLYPFAQTVAREGCSLEDAVENIDIGGPTMVRSAAKNHKDVAIVVKSSDYDAIIKEM
                     DANEGSLLLATRFDLAIKAFEHTAAYDSMIANYFGSMVPAYHGESKEAAGRFPRTLNL
                     NFIKKQDMRYGENSHQQAAFYIEENVKEASVATATQVQGKALSYNNIADTDAALECVK
                     EFAEPACVIVKHANPCGVAIGHSILDAYDRAYKTDPTSAFGGIIAFNRELDAETAQAI
                     ISRQFVEVIIAPSASEEALKITAAKQNVRVLICGQWGERAPGLDFKRVNGGLLVQDRD
                     LGMVGAEELRVVTKRQPTEQELRDALFCWKVAKFVKSNAIVYAKNNMTIGIGAGQMSR
                     VYSAKIAGIKAADEGLEVKGSSMASDAFFPFRDGIDAAAAAGVTCVIQPGGSIRDDEV
                     IAAADEHGIAMLFTDMRHFRH"
BASE COUNT         6978 a         7719 c         8211 g         6886 t
ORIGIN      
        1 gtagagatgg taatgccggg cgacaacatc aaaatggttg ttaccctgat ccacccgatc
       61 gcgatggacg acggtctgcg tttcgcaatc cgtgaaggcg gccgtaccgt tggcgcgggc
      121 gttgtagcaa aagttctgag ctaattgccg ataacatttg acgcaatgcg cactaaaagg
      181 gcatcatttg atgccctttt tgcacgcttt cgtaccagaa cctggctcat cagtgatttt
      241 ctttgtcata atcattgctg agacaggctc tgttgagggc gtataatccg aaaagctaat
      301 acgcgtttcg atttggtttg cctcgcgatc gcggggtgaa aatgtttgta gaatacttct
      361 gacaggttgg tttatgagtg cgaataccga agctcaagga agcgggcgcg gcctggaagc
      421 gatgaagtgg gtcgttgtgg tggcattgct cctggtagcg attgtcggca actaccttta
      481 tcgcgacatt atgctgccgc tgcgtgcgct ggccgtagta attctgattg ctgcagcggg
      541 tggtgtcgcg ctgttaacga caaaaggtaa agcaaccgtt gcttttgccc gtgaagcgcg
      601 taccgaagtc cgtaaggtca tttggccgac tcgccaggaa acattgcaca ccacgctgat
      661 tgtggctgcg gttaccgcag taatgtcact gatcctgtgg ggactggatg gtattctggt
      721 tcgcctggta tcctttatca ctggcctgag gttctgagat gtctgaagct cctaaaaagc
      781 gctggtacgt cgttcaggcg ttttccggtt ttgaaggccg cgtagcaacg tcgctgcgtg
      841 agcatatcaa attacacaac atggaagagt tgtttggtga agtcatggta ccaaccgaag
      901 aagtggttga aatccgtggc ggtcagcgtc gcaaaagcga acgtaaattc ttccctggct
      961 acgtcctcgt tcagatggtg atgaacgacg cgagctggca cctggtgcgc agcgtaccgc
     1021 gtgtgatggg cttcatcggc ggtacttccg atcgtcctgc gccaatcagc gataaagaag
     1081 tcgatgcgat tatgaaccgc ctgcagcagg ttggtgataa gccgcgtccg aaaacgttgt
     1141 ttgaaccggg tgaaatggtc cgtgttaatg atggtccgtt cgctgacttc aacggcgttg
     1201 ttgaagaagt tgattacgag aaatctcgtc tgaaagtgtc tgtttctatc ttcggtcgtg
     1261 cgacccctgt agagctggac ttcagccagg ttgaaaaagc ttaacccagc gatcaaaaaa
     1321 gcggcgattt aatcgttgca caaggcgcga gattggaata caatttcgcg ccttttgttt
     1381 ttatgggcct tgcccgtaaa acgatttttt atatcacggg gagcctctca gaggcgttat
     1441 tacccaactt gaggaattta taatggctaa gaaagtacaa gcctatgtca agctgcaggt
     1501 tgcagctggt atggctaacc cgagtccgcc agtaggtccg gctctgggtc agcagggcgt
     1561 aaacatcatg gaattctgca aagcgttcaa cgcaaaaact gattccatcg aaaaaggtct
     1621 gccgattccg gtagtaatca ctgtttacgc tgaccgttct ttcactttcg ttaccaagac
     1681 tccgccggca gcagttctgc tgaaaaaagc ggctggtatc aagtctggct ccggtaagcc
     1741 gaacaaagac aaagtgggta aaatttcccg cgctcagctg caggaaatcg cgcagaccaa
     1801 agctgccgac atgactggtg ccgacattga agcgatgact cgctccatcg aaggtactgc
     1861 acgttccatg ggcctggtag tggaggacta agaaatggct aaactgacca agcgcatgcg
     1921 tgttatccgc gagaaagttg atgcaaccaa acagtacgac atcaacgaag ctatcgctct
     1981 gctgaaagag ctggcgactg ctaaattcgt agaaagcgtg gacgtagctg ttaacctcgg
     2041 catcgacgct cgtaaatctg accagaacgt acgtggtgca actgtactgc cgcacggtac
     2101 tggccgttcc gttcgcgtag ccgtatttac ccagggtgca aatgctgaag ctgctaaagc
     2161 tgcaggtgct gaactggtag gtatggaaga tctggctgaa cagatcaaga aaggcgaaat
     2221 gaactttgac gttgttattg cttctccgga tgcaatgcgc gttgttggcc agctgggcca
     2281 ggttctgggt ccgcgcggcc tgatgccaaa cccgaaagtg ggtactgtaa cgccgaacgt
     2341 tgctgaagcg gttaaaaacg ctaaagctgg ccagattcgt taccgtaacg acaaaaacgg
     2401 catcatccac accaccatcg gtaaagtaga ctttgacgct gacaaactga aagaaaacct
     2461 ggaagctctg ctggttgcgc tgaaaaaagc aaaaccgact caggcgaaag gcgtgtacat
     2521 caagaaagtt agcatctcta ccactatggg tgcaggtgtt gcagttgacc aggctggcct
     2581 gagcgcttct gtaaactaat gcctttacgt gggcggtgat tttgtctaca atcttacccc
     2641 cacgtatgct aacgaaagtt agcagctaaa aagatttgtt cgttggagcc tggcctatcc
     2701 aggcctccgt cgaagaccgc aggagtttcg caagaaactt aatcccctgc gtagacggtg
     2761 acagaacgct aagattattc ttttatattc tggcttgttt ctgctcaccg taattaagac
     2821 gctctctccg tttggaggag tgaagtgagt tccagagatt ttctctggca aacatccagg
     2881 agcaaagcta atggctttaa atcttcaaga caaacaagcg attgttgctg aagtcagcga
     2941 agtagccaaa ggcgcgctgt ctgcagtagt tgcggattcc cgtggcgtaa ctgtagataa
     3001 aatgactgaa ctgcgtaaag caggtcgcga agctggcgta tacatgcgtg ttgttcgtaa
     3061 caccctgctg cgccgtgctg ttgaaggtac tccgttcgag tgcctgaaag acgcgtttgt
     3121 tggtccgacc ctgattgcat actctatgga acacccgggc gctgctgctc gtctgttcaa
     3181 agagttcgcg aaagcgaatg caaaatttga ggtcaaagcc gctgcctttg aaggtgagct
     3241 gatcccggcg tctcagatcg accgcctggc aactctgccg acctacgaag aagcaattgc
     3301 acgcctgatg gcaaccatga aagaagcttc ggctggaaaa ctggttcgta ctctggctgc
     3361 tgtacgcgat gcgaaagaag ctgcttaatc gcagttatct ttttaacgca ttcgcttacg
     3421 tataaactta ttctgatatt caggaacaat ttaaatgtct atcactaaag atcaaatcat
     3481 tgaagcagtt gcagctatgt ctgtaatgga cgttgtagaa ctgatctctg caatggaaga
     3541 aaaattcggt gtttccgctg ctgctgctgt agctgtagct gctggcccgg ttgaagctgc
     3601 tgaagaaaaa actgaattcg acgtaattct gaaagctgct ggcgctaaca aagttgctgt
     3661 tatcaaagca gtacgtggcg caactggcct gggtctgaaa gaagctaaag acctggttga
     3721 atctgcaccg gctgctctga aagaaggcgt gagcaaagac gacgcagaag cactgaaaaa
     3781 agctctggaa gaagctggcg ctgaagttga agttaaataa gccaaccctt ccggttgcag
     3841 cctgagaaat caggctgatg gctggtgact ttttagtcac cagccttttt gcgctgtaag
     3901 gcgccagtag cgtttcacac tgtttgacta ctgctgtgcc tttcaatgct tgtttctatc
     3961 gacgacttaa tatactgcga caggacgccc gttctgtgta aatcgcaatg aaatggttta
     4021 agcgtgatag caacaggcat tgcggaaagt gttccatttt ccggtcaaca aaatagtgtt
     4081 gcacaaactg tccgctcaat ggacagatgg gtcgacttgt cagcgagctg aggaacccta
     4141 tggtttactc ctataccgag aaaaaacgta ttcgtaagga ttttggtaaa cgtccacaag
     4201 ttctggatgt accttatctc ctttctatcc agcttgactc gtttcagaaa tttatcgagc
     4261 aagatcctga agggcagtat ggtctggaag ctgctttccg ttccgtattc ccgattcaga
     4321 gctacagcgg taattccgag ctgcaatacg tcagctaccg ccttggcgaa ccggtgtttg
     4381 acgtccagga atgtcaaatc cgtggcgtga cctattccgc accgctgcgc gttaaactgc
     4441 gtctggtgat ctatgagcgc gaagcgccgg aaggcaccgt aaaagacatt aaagaacaag
     4501 aagtctacat gggcgaaatt ccgctcatga cggacaacgg tacctttgtt atcaacggta
     4561 ctgagcgtgt tatcgtttcc cagctgcacc gtagtccggg ggtctttttt gactccgaca
     4621 aaggtaaaac ccactcttcg ggtaaggtgc tgtataacgc gcgcatcatc ccttaccgtg
     4681 gttcctggct ggacttcgaa ttcgatccga aggacaacct gttcgtacgt atcgaccgtc
     4741 gccgtaaact acctgcgaca atcattctgc gtgccctgaa ctacaccaca gagcagatcc
     4801 tcgacctgtt ctttgaaaaa gttatctttg aaatccgtga taacaagctg cagatggaac
     4861 tggtgccgga acgcctgcgt ggtgaaaccg catcttttga catcgaagct aacggtaaag
     4921 tgtacgtaga aaaaggccgc cgcattactg cgcgccatat tcgccaactg gaaaaagacg
     4981 acgtcaagct gatcgaagtc ccggttgagt acatcgcagg taaagtggtt gctaaagact
     5041 acattgatga gtctaccggc gagctgatct gcgcagcgaa catggagctg agcctggatc
     5101 tgctggctaa gctgagccag tctggtcata agcgtatcga aacgctgttc accaacgatc
     5161 tggatcacgg cccatatatc tctgaaacct tacgtgtcga cccaactaac gaccgtctga
     5221 gcgcactggt agaaatctac cgcatgatgc gccctggcga gccgccgact cgtgaagcag
     5281 ctgaaagcct gttcgagaac ctgttcttct ccgaagaccg ttatgacctg tctgcggttg
     5341 gtcgtatgaa gttcaaccgt tctctgctgc gcgaagaaat cgaaggttcc ggtatcctga
     5401 gcaaagacga catcattgat gttatgaaaa agctcatcga tatccgtaac ggtaaaggcg
     5461 aagtcgatga tatcgaccac ctcggcaacc gtcgtatccg ttccgttggc gaaatggcgg
     5521 aaaaccagtt ccgcgttggc ctggtacgtg tagagcgtgc ggtgaaagag cgtctgtctc
     5581 tgggcgatct ggataccctg atgcctcagg atatgatcaa cgctaagccg atttccgcag
     5641 cagtgaaaga gttcttcggt tccagccagc tgtctcagtt tatggaccag aacaacccgc
     5701 tgtctgaaat tacgcacaaa cgtcgtatct ccgcactcgg cccgggtggt ctgacccgtg
     5761 aacgtgcagg cttcgaagtt cgagacgtac acccgactca ctacggtcgc gtatgtccaa
     5821 tcgaaacccc tgaaggtccg aacatcggtc tgatcaactc tctgtccgtg tacgcacaga
     5881 ctaacgaata cggcttcctt gagactccgt atcgtaaagt gaccgacggt gttgtaactg
     5941 acgaaattca ctacctgtct gctatcgaag aaggcaacta cgttatcgcc caggcgaact
     6001 ccaacctgga tgaagaaggc cacttcgtag aagacctggt aacttgccgt agcaaaggcg
     6061 aatccagctt gttcagccgt gaccaggttg actacatgga cgtatccacc cagcaggtgg
     6121 tatccgtcgg tgcgtccctg atcccgttcc tggaacacga tgacgccaac cgtgcattga
     6181 tgggtgcgaa catgcaacgt caggccgttc cgactctgcg cgctgataag ccgctggttg
     6241 gtacgggtat ggaacgtgct gttgccgttg actccggtgt aactgcggtt gctaaacgtg
     6301 gtggtgtcgt tcagtacgtg gatgcttccc gtatcgttat caaagttaac gaagacgaga
     6361 tgtatccggg tgaagcaggt atcgacatct acaacctgac caaatacacc cgttctaacc
     6421 agaacacctg tattaaccag atgccgtgtg tgtctctggg tgaaccggtt gaacgtggcg
     6481 acgtgctggc agacggtccg tccaccgacc tcggtgaact ggcgcttggt cagaacatgc
     6541 gcgtagcgtt catgccgtgg aatggttaca acttcgaaga ctccatcctc gtatccgagc
     6601 gtgttgttca ggaagaccgt ttcaccacca tccacattca ggaactggcg tgtgtgtccc
     6661 gtgacaccaa gctggggccg gaagagatca ccgctgacat cccgaacgtg ggtgaagctg
     6721 cgctctccaa actggatgaa tccggtatcg tttacattgg tgcggaagtg accggtggcg
     6781 acattctggt tggtaaggta acgccgaaag gtgaaactca gctgacccca gaagaaaaac
     6841 tgctgcgtgc gatcttcggt gagaaagcgt ctgacgttaa agactcttct ctgcgcgtac
     6901 caaacggtgt atccggtacg gttatcgacg ttcaggtctt tactcgcgat ggcgtagaaa
     6961 aagacaaacg tgcgctggaa atcgaagaaa tgcagctcaa acaggcgaag aaagacctgt
     7021 ctgaagaact gcagatcctc gaagcgggtc tgttcagccg tatccgtgct gtgctggtag
     7081 ccggtggcgt tgaagctgag aagctcgaca aactgccgcg cgatcgctgg ctggagctgg
     7141 gcctgaccga cgaagagaaa caaaatcagc tggaacagct ggctgagcag tatgacgaac
     7201 tgaaacacga gttcgagaag aaactcgaag cgaaacgccg caaaatcacc cagggcgacg
     7261 atctggcacc gggcgtgctg aagattgtta aggtatatct ggcggttaaa cgccgtatcc
     7321 agcctggtga caagatggca ggccgtcatg gtaacaaggg tgtaatttct aagatcaacc
     7381 cgatcgaaga tatgccttac gatgaaaacg gtacgccggt agacatcgta ctgaacccgc
     7441 tgggcgtacc gtctcgtatg aacattggtc agatcctcga aacccacctg ggtatggctg
     7501 cgaaaggtat cggcgacaag atcaacgcca tgctgaaaca gcagcaagaa gtcgcgaaac
     7561 tgcgcgaatt catccagcgt gcgtacgatc tgggcgctga cgttcgtcag aaagttgacc
     7621 tgagtacctt cagcgatgaa gaagttatgc gtctggctga aaacctgcgc aaaggtatgc
     7681 caatcgcaac gccggtcttc gacggtgcga aagaagcaga aattaaagag ctgctgaaac
     7741 ttggcgacct gccgacttct ggtcagatcc gcctgtacga cggtcgcact ggtgaacagt
     7801 tcgagcgtcc ggtaaccgtt ggttacatgt acatgctgaa actgaaccac ctggtcgacg
     7861 acaagatgca cgcgcgttcc accggttctt atagcctggt tactcagcag ccgctgggtg
     7921 gtaaggcaca gttcggtggt cagcgtttcg gggagatgga agtgtgggcg ctggaagcat
     7981 atggcgcagc atacaccctg caggaaatgc tcaccgttaa gtctgatgac gtgaacggtc
     8041 gtactaagat gtataaaaac atcgtggacg gcaaccatca gatggagccg ggcatgccag
     8101 aatccttcaa cgtattgttg aaagagattc gttcgctggg tatcaacatc gaactggaag
     8161 acgagtaatt ctcgctcaaa caggtcactg ctgtcggggt aaaacccggc agcggattgt
     8221 gctaactccg acgggagcaa atccgtgaaa gatttattaa agtttctgaa agcgcagact
     8281 aaaaccgaag agtttgatgc gatcaaaatt gctctggctt cgccagacat gatccgttca
     8341 tggtctttcg gtgaagttaa aaagccggaa accatcaact accgtacgtt caaaccagaa
     8401 cgtgacggcc ttttctgcgc ccgtatcttt gggccggtaa aagattacga gtgcctgtgc
     8461 ggtaagtaca agcgcctgaa acaccgtggc gtcatctgtg agaagtgcgg cgttgaagtg
     8521 acccagacta aagtacgccg tgagcgtatg ggccacatcg aactggcttc cccgactgcg
     8581 cacatctggt tcctgaaatc gctgccgtcc cgtatcggcc tgctgctcga tatgccgctg
     8641 cgcgatatcg aacgcgtact gtacttcgaa tcctatgtgg ttatcgaagg cggtatgacc
     8701 aacctggaac gtcagcagat cctgactgaa gagcagtatc tggacgcgct ggaagagttc
     8761 ggtgacgaat tcgacgcgaa gatgggtgcg gaagcaatcc aggctctact gaagagcatg
     8821 gatctggagc aagagtgcga acagctgcgt gaagagctga acgaaaccaa ctccgaaacc
     8881 aagcgtaaaa agctgaccaa gcgtatcaaa ctgctggaag cgttcgttca gtctggtaac
     8941 aaaccagagt ggatgatcct gaccgttctg ccggtactgc cgccagatct gcgtccgctg
     9001 gttccgctgg atggtggtcg tttcgcgact tctgacctga acgatctgta tcgtcgcgtc
     9061 attaaccgta acaaccgtct gaaacgactg ctggacctgg ctgcgccgga tatcatcgta
     9121 cgtaacgaaa aacgtatgct gcaggaagcg gtagacgccc tgctggataa cggtcgtcgc
     9181 ggtcgtgcaa tcaccggttc taacaagcgt cctctgaaat ctttggccga catgatcaaa
     9241 ggtaaacagg gtcgtttccg tcagaacctg ctcggtaagc gcgttgacta ctccggtcgt
     9301 tctgtaatca ccgtaggtcc atacctgcgt ctgcatcagt gcggtctgcc gaagaaaatg
     9361 gcgctggagc tgttcaaacc gttcatctac ggcaagctgg aactgcgtgg tcttgctacc
     9421 accatcaaag ctgcgaagaa aatggttgag cgcgaagaag ctgtcgtttg ggatatcctg
     9481 gacgaagtga tccgcgaaca cccggtactg ctgaaccgtg caccgactct gcaccgtctg
     9541 ggtatccagg catttgaacc ggtactgatc gaaggtaaag ctatccagct gcacccgctg
     9601 gtttgtgcgg catataacgc cgacttcgat ggtgaccaga tggctgttca cgtaccgctg
     9661 acgctggaag cccagctgga agcgcgtgcg ctgatgatgt ctaccaacaa catcctgtcc
     9721 ccggcgaacg gcgaaccaat catcgttccg tctcaggacg ttgtactggg tctgtactac
     9781 atgacccgcg actgtgttaa cgccaaaggc gaaggcatgg tgctgactgg cccgaaagaa
     9841 gcagaacgtc tgtatcgctc tggtctggct tctctgcatg cgcgcgttaa agtgcgtatc
     9901 accgagtatg aaaaagatgc taacggtgaa ttagtagcga aaaccagcct gaaagacacg
     9961 actgttggcc gtgccattct gtggatgatt gtaccgaaag gtctgcctta caccatcgtc
    10021 aaccaggcgc tgggtaaaaa agcaatctcc aaaatgctga acacctgcta ccgcattctc
    10081 ggtctgaaac cgaccgttat ttttgcggac cagatcatgt ataccggttt tgcatatgca
    10141 gcgcgttctg gtgcatctgt tggtatcgat gacatggtca tcccggagaa gaaacacgaa
    10201 atcatctccg aggcagaagc agaagttgct gaaattcagg agcagttcca gtctggtctg
    10261 gtaactgcgg gcgaacgcta caacaaagtt atcgatatct gggctgcggc gaacgatcgt
    10321 gtatccaaag cgatgatgga taacctgcaa actgaaaccg ttattaaccg tgacggtcag
    10381 gaagagaagc aggtttcctt caacagcatc tacatgatgg ccgactccgg tgcgcgtggt
    10441 tctgcggcac agattcgtca gcttgctggt atgcgtggtc tgatggcgaa gccggatggc
    10501 tccatcatcg aaacgccaat caccgcgaac ttccgtgaag gtctgaacgt actccagtac
    10561 ttcatctcca cccacggtgc tcgtaaaggt ctggcggata ccgcactgaa aacggcgaac
    10621 tccggttacc tgactcgtcg tctggttgac gtggcgcagg acctggtggt taccgaagac
    10681 gattgtggta ctcatgaagg tatcatgatg actccggtta tcgaaggtgg tgacgttaaa
    10741 gagccgctgc gcgatcgcgt attgggtcgt gtaactgctg aagacgttct gaagccgggt
    10801 actgctgata tcctcgttcc gcgcaacacg ctgctgcacg aacagtggtg tgacctgctg
    10861 gaagagaact ctgtcgacgc ggttaaagta cgttctgttg tatcttgtga caccgacttt
    10921 ggtgtatgtg cgcactgcta cggtcgtgac ctggcgcgtg gccacatcat taacaaaggt
    10981 gaagcaatcg gtgttatcgc ggcacagtcc atcggtgaac cgggtacaca gctgaccatg
    11041 cgtacgttcc acatcggtgg tgcggcatct cgtgcggctg ctgaatccag catccaggtg
    11101 aaaaacaaag gtagcatcaa gctcagcaac gtgaagtcgg ttgtgaactc cagcggtaaa
    11161 ctggttatca cttcccgtaa caccgaactg aaactgatcg acgaattcgg tcgtaccaaa
    11221 gaaagctaca aagtacctta cggtgcggta ctggcgaaag gcgatggcga acaggttgct
    11281 ggcggcgaaa ccgttgcaaa ctgggacccg cacaccatgc cggttatcac cgaagtaagc
    11341 ggttttgtac gctttactga catgatcgac ggccagacca ttactcgtca gaccgacgaa
    11401 ttgacaggtc tgtcttcgct ggtggttctg gattccgcag aacgtaccgc aggtggtaaa
    11461 gatctgcgtc cggcactgaa aatcgttgat gctcagggta acgacgttct gatcccgggt
    11521 accgatatgc ctgcgcagta cttcctgccg ggtaaagcga ttgttcagct ggaagatggc
    11581 gtacagatca gctctggtga caccctggcg cgtattccgc aggaatccgg cggtaccaag
    11641 gacatcaccg gtggtctgcc gcgcgttgcg gacctgttcg aagcacgtcg tccgaaagag
    11701 ccggcaatcc tggctgaaat cagcggtatc gtttccttcg gtaaagaaac caaaggtaaa
    11761 cgtcgtctgg ttatcacccc ggtagacggt agcgatccgt acgaagagat gattccgaaa
    11821 tggcgtcagc tcaacgtgtt cgaaggtgaa cgtgtagaac gtggtgacgt aatttccgac
    11881 ggtccggaag cgccgcacga cattctgcgt ctgcgtggtg ttcatgctgt gactcgttac
    11941 atcgttaacg aagtacagga cgtataccgt ctgcagggcg ttaagattaa cgataaacac
    12001 atcgaagtta tcgttcgtca gatgctgcgt aaagctacca tcgttaacgc gggcagctcc
    12061 gacttcctgg aaggtgaaca ggttgaatac tctcgcgtca agatcgcaaa ccgcgaactg
    12121 gaagcgaacg gcaaagtggg tgcaacttac tcccgcgatc tgctgggtat caccaaagcg
    12181 tctctggcaa ccgagtcctt catctccgcg gcatcgttcc aggagaccac tcgcgtgctg
    12241 accgaagcag ccgttgcggg caaacgcgac gaactgcgcg gcctgaaaga gaacgttatc
    12301 gtgggtcgtc tgatcccggc aggtaccggt tatgcgtacc accaggatcg tatgcgtcgc
    12361 cgtgctgcgg gtgaagctcc ggctgcaccg caggtgactg cagaagacgc atctgccagc
    12421 ctggcagaac tgctgaacgc aggtctgggc ggttctgata acgagtaatc gttaatccgc
    12481 aaataacgta aaaacccgct tcggcgggtt tttttatggg tgttattcag agggcaaagt
    12541 cttcacggaa tttttaagaa aggcagggcg aaacgaggaa gaagcttttc gccctggaca
    12601 acattcctgt tgacgggact accataaaaa aacacaacgc tttcaacaac cttgcgtttt
    12661 tcatcttccg gagctgctcg gaataaattt acgttcttac attctgtctc atggcctttg
    12721 cgatgggcgt cccagatagc tgtcccagtc tttccatacc ggctgcaaac cctgagcggt
    12781 taacgcggca gcaaccgctt ccggtctgcg atcgtcgtgc ggtgagaact gttccagctc
    12841 ggggtggttg tcggcatagc cacctggctg cgttttcgaa aaagcgctga cgttattaat
    12901 tgccagcgga attacgcgat cgcgaaacca cggtgattcc cgcgtggaga gtgacagttc
    12961 aatctccggt gcaagcagcc ggaaagcgca gatggcttgc actaactggc gttcatccat
    13021 aatcgacgca ggttcaatgc cgccagtaca tggacgcagg cgtgggaagg agacagagta
    13081 gcggctttgc cagtaatgct gttgcagcca tagcaaatgt tcggcaacca tatagcagtc
    13141 aactcgccag ctgtcggaaa ggccgattag cgcgccgagg cctatcttat caatccccgc
    13201 acgccctagc cgatccggcg tttccagccg ccagaagaag tcctgttttt tacctttcag
    13261 atgatgatgg gcataagtcg cctcattata tgtctcctga taaaccatta ctccatcgag
    13321 gccaagctgt tttaactcgg catattccgc ctccgccagc ggttgcactt ccatctgtag
    13381 tgaagagaac tgttcacgca gggcagggag atgacgacga aagtaatcca tccccacttt
    13441 cgcctgatgt tcaccagtga ctaatagcag atgttcaaag cccatctccc gtatagcggc
    13501 gctttccctg gcaatatccg cttcatccag cgttttgcgc ttgatgcggt tgctcataga
    13561 aaatccgcaa taagtacagt cgttagcgca aagattggaa agataaagcg ggacgtagaa
    13621 gctgacgacg ttgccaaatc gctgacgagt cagacgttgc gctcgctggg ccagtggttc
    13681 cagatagcca ctggcggcgg gtgatagcaa tgccatcatg tcgtcgcggg tgagttgcga
    13741 ggcatttagc gcccgctcta cgtcagcagc cgttttgccg ttgatacgca ggtggatgtc
    13801 atcccagtcc agttgtcgcc agcgatcgct gaaggttttc atgccgatgc ctccagaaat
    13861 ccagtcagcg ggctggtggc atgagcaaaa tgactgcggc tgcccggtcc ggactgacgt
    13921 gccagcaggc ctgcttctac cgccagacga aatgccttcg ccatgttgac gggatcgtcc
    13981 acgacggcaa tcgccgtatt cactaacacc gcgtcggccc ccatttccag cgcctgcgcg
    14041 gcatggctgg gaacgccgat gccagcatcg acaaccaccg gaacggtggc ctgctggata
    14101 ataatctcca gcatggcgcg ggtttccagt ccctgattcg agccaatcgg cgcgccgagc
    14161 ggcatcaccg ctgcacagcc gacttcttcc agacgtttac acaataccgg atcggccccg
    14221 cagtaaggca gcacgataaa tccctgttgt accagcgttt cggcggcttt cagggtttcg
    14281 atgggatcgg gcaacagcca gcgggcgtca gggtgaatct ccaattttag ccagtttgtg
    14341 cctaacgctt cacgtgccag atgggcggcg aaaatggctt cttccgcggt tttcgccccg
    14401 gaagtattcg gcagcagggt cacacccgcc gcgataagcg gttcgaggat agcgtcgttg
    14461 tgctggcgca agtcgacacg tttcatcgcc agcgtcacca gttggctgcc ggaagcgcga
    14521 atagactcca ccatcagttg tgaagaggcg aattttccgg tgccagtaaa cagatgtgaa
    14581 tcaaacgttt tgtccgcaat atgtaacatc tcaaccccct gcaataactt gaaaaagcag
    14641 gatctggtcg ccatcctgca cgatatgttg cgcccattgt tcccgcggga cgatttgctg
    14701 attaatcgct agtgccgcac ctgcttgttg ttgatcgagt tgctccagca gttcgtgaac
    14761 agttagtccg gcgacacact gcatcggttg atcgttaaac aggatctgca ttgcatcctc
    14821 cacataccgg gcaaccgttg gcgcggcgca acgtcaggct gcgccactgg ctcgatttac
    14881 cgtcgaacag tcggagttct cccgcaggcg tctctatacc gcttaataac ttaatcgctt
    14941 ccagtgcctg taaagtgccc ataaccccga ccaccgggcc aaccacgccc gccgtgcggc
    15001 agttgcgttc tggctcctgg ttatctggcc acaggcagcg gtaacatccc tgctcccagg
    15061 gcggcgtcag taccatcaac tgaccgccaa atccgaccgc gctggcggtg ataagcggcg
    15121 tgttgagtgc cacgcaggtg gcattaatct cctggcgagt cgccatattg tcagtacagt
    15181 cgagcaccac atcggcttgt gcaaccgcat cttttaacgt ctcacccgtt aatcgttgtt
    15241 gtaatgccat cagttgaatg tcgggattca actgtgtcag tcgctgttgg ctgacctgtg
    15301 atttcgggcg atcgatatct tcagtggtaa agaggatttg tcgttgcaga ttgcttaaat
    15361 gcacatcgtc gtcatctgcc agtaccagcg tcccgacgcc agcccccgcc aggtatagcg
    15421 cagcaggtgt acccagcccg ccaagaccaa taatcagcac ctggctgtcg agcagttttt
    15481 gctgcccgtc cagagcgata tcgtcgagca ggatttggcg gctataacgc ataaagtcac
    15541 ggtcattcat cgccaactcc tgcaatttcc agcagctgtg ccgttgccaa acgccagtct
    15601 acggcttgag taatggcgct gacgacggcg atactgccga cacccgttgc tatcaccgca
    15661 ggcgcgcgcg ccagactgat accgccaatc gccacggtgg gataatccgc caggcgctca
    15721 acatgccgtg ccagctgttc cagtccctgc ggtgcagaag gcatctgttt ggtttgtgtc
    15781 ggaaacacat gtcccagcgc gatataagaa ggacgtgccg ccagcgcgac gtcgatctcc
    15841 atatcgtcat gggtcgaaac gcccagccgc aggcctgccg cgcggatggc gctgagatcg
    15901 gtggcttgca aatcttcctg ccccagatgg acgccatacg cctgatgctt aatcgccagc
    15961 cgccagtaat cgttgataaa caatcgcgcg ttatagcggc ggcccagcgc aattgccgcc
    16021 accacatcgg cttctacctc ttcatcgcgc cgatctttga tgcgtagctg gagagtacgt
    16081 acgcctgcat ccaacagacg ttcgatccac tgtacgctgt caaccaccgg gtacagtcct
    16141 aaacgaaagg gtacaggagg aaactcaggc tgatacatca cgcttcctcc ttacgcaggt
    16201 agatttctcc gcctctggcg cggaagttct ccgacatatc cgccattccc acttcaatgg
    16261 tttgcgcggc ggcgtaatca cgcacttcct ggctgatttt catcgagcag aatttcggtc
    16321 cacacatgga gcaaaaatgg gcgactttac cggactcttg cggcagggtt tcatcgtgat
    16381 aagcgcgggc agtaaacggg tcgagggcca gattaaactg gtcttcccag cgaaattcga
    16441 agcgggcttt cgacatggcg ttatcgcgaa tttgcgcgcc cggatgccct ttcgccaggt
    16501 cggcggcgtg agcggcaatc ttataggtga taagcccctg tttaacatct tctttatttg
    16561 gcaggcccag atgctctttc ggcgttacgt agcagagcat tgcacaacca aaccagccaa
    16621 tcatcgccgc gccaatccct gacgtgaagt ggtcatagcc cggcgcgata tcggtagtta
    16681 gcggccccag agtgtaaaac ggcgcttcgt ggcagtgctc taactcctcg gtcatattgc
    16741 ggcgaatcat ctgcatcggc acgtggcctg ggccttcaat catcacctgt acgtcatatt
    16801 cccaggcgat tttagtcagt tcacccagcg tatgcagctc ggcaaactgt gcttcatcgt
    16861 tggcgtcctg aatagaaccg gggcgcaggc cgtcgcccag cgatagcgaa acgtcataag
    16921 cagcacagat ttcacaaatt tcgcggaagt gttgatagag gaaattttcc tgatgatggg
    16981 agaggcacca tttcgccata atcgaaccgc cgcgagagac gataccggtc aggcgtttcg
    17041 cggtcatcgg cacgtagcgc agcagcaccc ccgcatggat cgtgaagtaa tccacacctt
    17101 gctcggcttg ttccagcagg gtgtcgcgga agacttccca ggtaagatct tcagcgatcc
    17161 cgttcacctt ctccagtgcc tggtagatcg gcactgtacc gatcggcacc gggctgttac
    17221 gcaaaatcca ctcgcgggtt tcgtgaatat agcgaccagt ggaaagatcc atcacggtat
    17281 ccgctcccca gcgtgtggac cataccagtt tttccacttc ttcttcgatg gaagaagtga
    17341 ccgctgagtt gccgatattg gcgttaactt ttaccaggaa gttacggcca ataatcatcg
    17401 gctccgattc cggatgatta atgttggccg ggataatcgc acgtccggca gcaacttcat
    17461 cacggacaaa ttccgcagtg atattttccg gcagacgtgc gccaaagctc attcccggat
    17521 gctggtggcg taaaacttcg ctacgtatgc gctcgcgacc catattctcg cgaatggcga
    17581 tgaattccat ttcgggcgtg atgataccct ggcgggcgta gtgcagttgg gtgacgcggc
    17641 gtcctgcttt ggcgcgtttt ggcgttaata cgccgctaaa acgcagctcg tcgaggccat
    17701 catctgccag ccgcgctttt gtgtaatcgg aactgcgcac ggtaagttct tcggtatcgc
    17761 cgcgcgcatc gatccacggc tggcgtagtt ttgccagccc ttgctgcacg ttaatggcga
    17821 tctgcggatc gccatacgga ccggaggtgt cgtagaccgg aatcgcttcg ttttcttcgt
    17881 actgcggttg ttctttgcta ccgccgatta gcgtcgggct aagctggatc tcacgcatcg
    17941 gcacgcgtac gccggggtgt gtgccagtga tgtaaatgcg ttttgagttg ggaaaggcgg
    18001 agccttccag ggtgtcgata aaatgttggg cctgggcgcg ctgttcgcgg cgggtcagtt
    18061 ttgttgcaga catagctcat tccaaaaagt taaggacgtg gcttgtcaga cgacggatga
    18121 agcaagagac gatcgccgca aaggcgatgc gatagcagat taactcttgt tcccttcgca
    18181 ggtattagcc tgatcaggtt ccgcggatcc cgaataaacg gtctcagcca gttaaggcac
    18241 tccgacaaga aattagcccc acaaacgggg cgttgaatgt aaattacgcg ttaacagcgc
    18301 agaactcaag caggatgttt gacgagggcg gcgtcaagca ccagtagaat cagcttatct
    18361 tccagcacaa agcgcgcttc cagtgcctcg ccgatgtcag ataaaacctg ttgaaactca
    18421 agataattat catgatcgat ggcggtttcc agactggaat cgtagtcatc cataatctgt
    18481 tgagtattgg cttcgagttg tggccaaatc ttcgcggcgc gtgcgagttg cccattgcct
    18541 tccagcttat gaagaatgcg ctcataaata ctgaaatgtc cggcagacaa gtaatcgacc
    18601 aggctctgac aaaaatcatc aagggctttt tcgtttagcc tcatgtacga ttctttgcca
    18661 ggcttaatgc caaccagatt gtagtaagcc acgagcagat gcttacgtac atgtagccag
    18721 cgatcaacca gtttgttact tcctctgacg cgctccgtca ggttatcgag ctggttaagc
    18781 atgattgact ccgcaagttt gtattcaaaa actgctctgt gagaaatgta aaaaccatgt
    18841 taaacatgcc agtgatgcaa aggtagtgca agagctatgg atcgtataat tgaaaaatta
    18901 gatcacggct ggtgggtcgt cagccatgaa caaaaattat ggttgccgaa gggagaattg
    18961 ccatatggcg aagcggcaaa tttcgatctt gtgggtcagc gcgcactaca aatcggcgaa
    19021 tggcaggggg aacctgtttg gttagtacaa ctgcagcggc gtcacgatat ggggtcggta
    19081 cgtcaggtca ttgatctcga tgttgggctg tttcaactgg ccggacgtgg cgtgcaactg
    19141 gcggagtttt accgatcgca taaatactgt ggttactgcg ggcatgaaat gtatccaagc
    19201 aaaaccgaat gggcgatgct gtgcagccat tgccgtgagc gttactaccc gcaaatcgcc
    19261 ccctgcatta ttgttgccat ccgtcgcgat gattcgatcc tcctcgccca gcatacccgt
    19321 catcgtaacg gtgtccatac agtacttgcc ggattcgtcg aagtgggtga aaccctcgag
    19381 caggcagtcg cgcgggaagt gatggaagag agcggaatta aagttaaaaa cttgcgttac
    19441 gtgacctccc aaccgtggcc gtttcctcag tcgttaatga ccgcgtttat ggcggaatat
    19501 gacagcggcg agatcgtgat cgacccgaaa gaattgcttg aggcgaactg gtatcgctat
    19561 gacgatttgc cgttactccc gccgcccggc accgtagcgc gccgtctgat agaagataca
    19621 gtggcgatgt gtcgggcaga gtatgagtga tgatacactg accgcctgac gcactaagga
    19681 acagccaaaa tgaccgaact taaaaacgat cgttatctgc gggcgctgct gcgccagccc
    19741 gttgatgtca ctccagtatg gatgatgcgc caggcgggtc gctatctacc ggaatataaa
    19801 gccacgcgcg cccaggcggg cgattttatg tcgctgtgca aaaatgccga gctggcgtgc
    19861 gaagtgactt tgcaaccgct gcgtcgctat ccgctggatg cggcgatcct cttttccgat
    19921 atcctcaccg tgccggacgc gatggggtta gggctctatt ttgaagccgg agaaggtccg
    19981 cgttttacct cgccagtcac ctgcaaagcc gacgtcgata aactgccaat tccggacccg
    20041 gaagatgagc tgggttacgt gatgaacgcg gtgcgtacca ttcgtcgcga actgaaaggc
    20101 gaagtgccgc tgattggttt ttccggcagc ccgtggacgc tggcgactta catggttgaa
    20161 ggcggtagca gcaaagcctt caccgtgatc aaaaagatga tgtatgccga tccgcaggcg
    20221 ctgcacgcgc tgctcgataa actggcgaaa agcgtcacgt tgtatctgaa tgcgcagatt
    20281 aaagccggtg ctcaggcagt catgattttc gacacctggg gcggcgtgct taccgggcgc
    20341 gattatcaac agttctcgct ttattacatg cataaaattg ttgatggttt actgcgtgaa
    20401 aacgacggtc gccgcgtgcc agtcacgctg tttaccaaag gtggcggaca gtggctggaa
    20461 gcgatggcag aaaccggttg cgatgcgctg ggcctcgact ggacaacgga catcgccgat
    20521 gcgcgccgtc gcgtgggcaa taaagtcgcg ctgcagggta atatggaccc gtcgatgctg
    20581 tacgcgccgc ctgcccgcat tgaagaagaa gtggcgtcta tacttgccgg tttcggtcac
    20641 ggcgaaggcc atgtctttaa ccttggtcac ggcattcatc aggatgtgcc gccagaacat
    20701 gcaggcgtgt ttgtggaggc agtgcatcgc ttgtctgaac agtatcaccg ctaaggagcg
    20761 attatggatc tcgcgtcatt acgcgctcaa caaattgaac tggcttcttc tgtgatccgc
    20821 gaggatcgac tcgataaaga tccgccggat ctgatcgccg gagccgatgt cggatttgag
    20881 cagggcggcg aagtgacgcg agcggcgatg gtgctgctga aatacccctc gcttgagctg
    20941 gtggagtata aagtggcccg catcgccacc accatgcctt acattcctgg ttttctctcc
    21001 tttcgtgaat atcctgcgct gctggcagcg tgggagatgc tgtcacaaaa gccggattta
    21061 gtgtttgtcg atggtcatgg gatctcgcat cctcgccgtc ttggcgtcgc cagccatttt
    21121 ggtttgctgg tggatgtgcc gaccattggc gtagcgaaaa aacggctctg cggtaaattc
    21181 gaaccgctct ccagcgaacc gggcgcgctg gccccgctga tggataaagg cgagcagctg
    21241 gcctgggtct ggcgcagcaa agcgcgctgt aacccgttgt ttatcgctac cggccatcgg
    21301 gtcagcgtgg acagcgcgct ggcgtgggta caacgctgca tgaaaggcta tcgtctgccg
    21361 gagccaacgc gctgggcgga cgcggtggcc tcggaacgtc cggcgttcgt gcgctataca
    21421 gcaaatcagc cctaattcag gtaaactgcg gataatttcc gtatttgaga actcaacatg
    21481 ttacaaaacc caattcatct gcgtctggag cgcctggaaa gctggcagca cgtcactttt
    21541 atggcctgct tatgcgaacg tatgtacccc aattacgccg tattctgtca gcaaaccggc
    21601 ttcggcgatg ggcagattta ccgccgcatt ctcgatctca tttgggaaac gctgaccgtc
    21661 aaagacgcaa aagtaaattt cgacagccaa ctggagaaat ttgaagaagc gattccttca
    21721 gccgacgatt tcgatctgta cggcgtttat ccggcaatcg atgcttgcgt ggcgttaagt
    21781 gaactggtcc attcgcgttt aagtggcgaa acgctcgaac acgcggtgga agtgagtaag
    21841 acttccatca cgaccgtggc gatgctggaa atgactcagg ctggtcgcga aatgagcgat
    21901 gaagagctca aagaaaaccc agctgtagag caagaatggg acattcagtg ggaaatattc
    21961 cgacttttag ctgagtgcga agaacgcgat atcgagctga taaaaggcct tagggcagac
    22021 ctgcgtgagg cgggtgagag caatattggt ataatttttc agcaataaga ccagaaaacg
    22081 tgatttaacg cctaatttgt cgtacctaga gtcttccctt tcgccccccg tctggtctac
    22141 atttgggggg cgaaaaaaag tggctatcgg tgcgtgtatg caggagagtg ctattctggc
    22201 atttccgtcg cactcgatgc ttagcaagcg ataaacacat tgtaaggata acttatgaac
    22261 aagactcaac tgattgatgt aattgcagag aaagcagaac tgtccaaaac ccaggctaaa
    22321 gctgctctgg agtccactct ggctgcaatt actgagtctc tgaaagaagg cgatgctgta
    22381 caactggttg gtttcggtac cttcaaagtg aaccaccgcg ctgagcgtac tggccgcaac
    22441 ccgcagaccg gtaaagaaat caaaattgcc gcagctaacg taccggcatt tgtttctggc
    22501 aaggcactga aagacgcagt taagtaagat tgcgtggcag tgaacagttt taacgaaggg
    22561 gtggtttcac cccttttgtc tttctggcgt cgatcattga tgctggcggg cgctctgttt
    22621 cttactgcct gtagtcacaa ctcttcactt cctcccttta ccgccagtgg atttgctgaa
    22681 gaccagggcg cggtacgcat ctggcgaaaa gacagcggcg ataatgtgca tctgcttgcc
    22741 gtgtttagcc cgtggcgcag tggcgatacc acaacgcgag agtatcgctg gcagggcgat
    22801 aacctcacgc tcatcaatat caatgtttac agcaaaccgc cggtgaacat tcgcgcgcgt
    22861 tttgacgatc gcggtgatct gagctttatg caacgtgaat ccgatgggga aaaacagcag
    22921 ctttctaacg accaaatcga tttataccgt tatcgtgctg atcagatccg ccagattagc
    22981 gatgccttac gtcaggggag agtcgtgctg cgccaggggc gctggcatgc gatggaacag
    23041 accgtgacca cctgcgaagg gcaaaccatt aaacctgatt tagattcgca ggcgatagcg
    23101 catatcgagc gccgccagag ccgctcttct gttgatgtca gcgtggcatg gctggaagcg
    23161 cccgaaggtt cgcaattact gttagtggca aactctgatt tctgtcgctg gcaacccaac
    23221 gagaaaacgt tctgatttac cagtggccca cacccatatg accgccacca ccgcagccgc
    23281 cgtagcccat gcccattccg gctccgcgcg gaatacccgc ttcagccatc gcaatatccc
    23341 gtttcacccg taactcatct aacgactgac gcaaattctc catctctttg gcgaccgcgt
    23401 taattttgct gctatccggt gggttcgcgg ctaacagggc attgtattca taacgctttg
    23461 tcaccagttg ctgttgcagt gcgctgcttt gagcgtaaaa gtcattatgg attttctgcc
    23521 acgctgtctg ttgttcgctg gtcaaaggcg cggcattttg ctgccacata ccatgtccgc
    23581 cgtgagcaaa tgcagatgtc gatcccatcg ccattgctga aagcgccatc attaccaggg
    23641 caattttcgt gttccgtttc atggttaatc ctccagtggt tgtcttactt cggatattgc
    23701 atcttccgtg ccaacgatga aacgctgata tgacgggtaa tctggcatga taaacgagta
    23761 aaaatgactc gcctgttgtg ggtagcgagt catttttact cattgaaact taagcctttg
    23821 tgttacagcg cagggtaagc gctgataaaa gatggcatga tttctgctgt cagaaaggga
    23881 tgagcaggca aagaagaaga tgcgttttat gcaacgttct aaagactcct tagctaaatg
    23941 gttaagcgcg atcctcccca tggtcattgt tgggctggtg gggctgtttg cggtgacagt
    24001 gattcgcgat tatgggcgcg agactgccgc cgccagacaa acgctgctgg aaaaaggcag
    24061 tgtacttatt cgtgctctcg aatccggctc gcgcgtcggc atggggatgc gcatgcatca
    24121 tgcgcagcag caggcattac tggaagaaat ggccgggcag cctggtgtac gttggtttgc
    24181 ggtcacggat gaacaaggaa caatcgtgat gcatagcaac tccggcatga tgggaaaaca
    24241 gctttattcc ccgcaggaaa tgcagcagtt acatccggga gatgaagaag cgtggcggcg
    24301 gatcgatagc gcagacggcg agcctgttct ggaaatttat cgccagtttc aaccgatgtt
    24361 tgctgctgga atgcaccgga tgcgccatat gcagcagtat gccgcgacac cacaagcaat
    24421 ttttatcgct ttcgacgcca gtaacattgt gagtgctgaa gatcgtgagc agagaaacac
    24481 cctgattatc ctcttcgccc tggcgacggt cttgctggca agcgtgttgt cattcttctg
    24541 gtatcgccga tatctgcgct cgcggcagct attgcaggat gaaatgaagc gcaaagagaa
    24601 gctggtggca ctggggcatc tggcggcagg cgttgcccac gaaatccgta acccactttc
    24661 ctcgattaaa gggctggcga aatactttgc cgaacgcgcg ccagcagggg gagaagcgca
    24721 tcaactggcg caggtgatgg cgaaagaagc tgaccgttta aaccgcgtgg taagcgagtt
    24781 gctggaactg gttaagccaa cgcatctggc tttgcaggcg gtggaactca acacgctgat
    24841 tagccactca ttacagctgg taagccagga tgcaaacagc cgggagatcc agttacgctt
    24901 taccgccaac gacacattac cggaaattca ggccgatccg gacaggctga ctcaggtcct
    24961 gttgaatctc tatctcaatg ctattcaggc gattggtcag catggcgtga ttagcgtgac
    25021 ggccagcgaa agcggcgcag gcgtgaaaat cagcgttacc gacagcggta agggaattgc
    25081 ggcggatcag cttgaagcca tcttcacccc gtacttcacc accaaagccg aaggcaccgg
    25141 actggggctg gcggtcgtgc ataatattgt tgaacaacac ggtggtacaa ttcaggtcgc
    25201 aagccaggag ggaaaaggcg caacgttcac cctttggctt ccggtcaata ttacgcgtaa
    25261 ggacccacaa ggatgacgca cgataatatc gatattctgg tggtggatga tgacattagc
    25321 cactgcacta ttttgcaggc tttgctgcgc ggctggggtt ataacgtcgc gctggcgaac
    25381 agcgggcgac aggcgctgga gcaggtgcgg gaacaggttt ttgatcttgt gctttgcgat
    25441 gtacgaatgg cagagatgga tggtatcgcc acgctaaaag agatcaaaac attaaacccc
    25501 gcgattccgg tgctgattat gaccgcgtac tccagcgtcg agacggcggt agaggcgttg
    25561 aaaaccgggg cgctggatta tctcatcaag ccgctggatt tcgataacct gcaggcgacg
    25621 ctggaaaagg cgctcgcgca tacacacagt gttgacgctg aaacgcctgc ggtgtctgcc
    25681 agccagttcg gtatggtcgg taaaagcccg gcgatgcaac acctgctcag tgaaatcgcc
    25741 ctcgtcgcgc catcggaagc cacggtgctg atccacggcg attccggcac cggtaaagaa
    25801 ctggtcgcca gggcgattca cgccagtagc gcacgtagcg aaaaatcgct ggtaacgctc
    25861 aactgcgcgg cgctcaacga atccttactg gagtctgaat tgttcggtca cgaaaaaggg
    25921 gcgtttaccg gggccgacaa acggcgggag gggcgctttg ttgaggcgga cggcggcacg
    25981 ttgtttctcg atgaaattgg cgatatctcg ccgatgatgc aggtgcgtct gctacgtgcg
    26041 attcaggagc gcgaagttca gcgtgtcggt agcaaccaga ctatttcggt tgatgtccgg
    26101 ctgattgcgg cgactcatcg cgatcttgcc gcagaggtga atgccgggcg ttttcgccag
    26161 gatctctact atcgcctgaa cgtggtggcg attgaagttc cgtcgctgcg tcagcggcgg
    26221 gaagatattc ctctgctggc aaaccatttt cttcagcgct ttgccgagcg taatcgcaag
    26281 gcggtaaaag gttttacgcc ccaggcgatg gatctgctga ttcactacga ctggccggga
    26341 aatattcgtg aactggaaaa cgcggtggaa cgggcggtgg tgctgctgac cggggaatat
    26401 atttccgaac gcgagctgcc gctggcgatt gccagtacgc cgatcccgct ggtacaaagt
    26461 caggatattc agccgctggt ggaagtcgaa aaagaggtga ttctggcggc gctggagaaa
    26521 acgggcggca acaaaaccga agccgcccgc cagttaggga tcacgcgcaa aacgctattg
    26581 gcaaaactgt cgcgttagtt ctgctcgcgt tcgatagcgc gccagccgat atctttccgg
    26641 cagaagcagt cgtcccaatg gatatcggtc attaaggcat aagcgcgttt ctgcgcttct
    26701 gccacggtat gacccagcgc ggtaacgcac agtacgcgcc cgccgctggt cactacctgc
    26761 tcgtcatccg ctagtttcgt gcccgcgtgg aacactttgc cgtctgccac ttcttccagc
    26821 ggcaggccgt ggatcacatc gccggtgcgg taatcgcccg gatatccacc cgcagccatc
    26881 accacgccga gagaagcgcg ttcatcccac tcggacgttt tctcgtccag cttgccttca
    26941 caggccgcca ggcagagctc aaccagatcg gacttcatgc gcagcataat cggctgggtt
    27001 tccggatcgc caaagcggca gttaaattca ataaccttcg gattgccctg tttgtcgatc
    27061 atcaggcccg cgtagagaaa accagtgtag gtgttgcctt ccgccgccat gcctttcacg
    27121 gttggccaga tgatacgttc catcgtgcgc tgatgaacct catcggtcac tactggcgca
    27181 ggggagtaag cgcccatccc gccggtgttc ggcccggtat ctttatcgcc tacgcgtttg
    27241 tgatcctggc tggtggccat cggcagcaca tgctcgccgt ccaccatcac gataaagctc
    27301 gcttcttcgc cgtcgaggaa ctcttcgata acaatgcgat gacccgcgtc gccaaacgcg
    27361 ttgcccgcca gcatgtcgcg cacggcggct tcagcttctt ccagcgtcat cgccacgata
    27421 acgcctttcc cggcggccag accgtccgct ttaatgacga ttggcgcgcc tttctcacgc
    27481 agatacgcca gcgcaggttc tacctcggtg aagttctggt attccgctgt gggaatgtta
    27541 tggcgggcca gaaagtcttt ggtgaatgct ttagaacctt ccagttgtgc agcacctgcg
    27601 gttgggccga agattttcag cccggcggcg cggaaggtat cgactacgcc tttcaccagc
    27661 ggcgcttccg ggccgacgat ggttagatct accttttcgt tttgtgcgaa atccagcagc
    27721 gccgggatat cggttacgcc gatagcgacg ttctgtagcg cgggttccag cgcagtgcct
    27781 gcattacccg gagcaacaaa aacagtttca accagcggtg actgagccgc tttccaggcc
    27841 agcgcgtgct cgcgcccgcc gttaccaatc actaatactt tcatctattg ctccattaat
    27901 ggcggaagtg gcgcatgtcg gtgaagagca tcgcaatacc gtgctcgtcg gcggcggcaa
    27961 tcacttcgtc atcacggatg gaaccgccag gctggattac acaggtcacg cccgcagcgg
    28021 cggcggcatc aataccgtcg cggaacggga agaacgcgtc agaagccatt gacgaacctt
    28081 tcacttccaa accttcatcg gcagctttga taccggcgat tttcgcggag tacacgcggc
    28141 tcatctggcc tgcgccaata ccgatggtca tattgttttt ggcatagacg atagcgttgg
    28201 atttcacgaa cttcgccact ttccagcaga acagcgcatc acgaagttcc tgttcggtcg
    28261 gctggcgttt ggtgacgacg cgcagttctt ctgcaccgac catccccagg tcgcgatcct
    28321 gaaccagcag accgccgttc acgcgtttga aatcaagacc cggagcacgc tcgccccact
    28381 gaccgcagat caggacgcga acgttctgtt tagcggcggt tattttcagg gcttcttcgc
    28441 tggcggacgg cgcaataatc acttcgacaa actgacgaga aatgatggcc tgtgcggttt
    28501 ccgcatccag ctcgcggtta aaggcgataa tgccgccgaa cgcggaggtt gggtcggttt
    28561 tgtacgcgcg atcgtaagca tcaagaatgg aatggccgat agccacgccg caagggttgg
    28621 cgtgcttcac aatcacacat gccggctcgg cgaactcttt cacgcactcc agcgccgcat
    28681 cggtatcggc gatgttgtta taagagaggg ctttaccctg aacctgggtt gcggtagcaa
    28741 cggaggcttc tttcacattc tcttctatat agaaggcagc ctgctggtgg ctgttctcgc
    28801 cgtaacgcat atcctgcttc ttaatgaagt tcaggttcag cgtgcgtggg aagcgaccgg
    28861 cggcttcttt gctttcaccg tggtaagccg gaaccatgct gccgaagtag ttggcaatca
    28921 tgctgtcgta ggcggcggta tgttcaaagg ctttgatagc gagatcgaaa cgggttgcaa
    28981 gcagaagcga tccttcgttg gcatccatct ctttaatgat ggcgtcgtag tcgctgctct
    29041 tcaccacgat ggcgacatct ttatggttct tggcggcgga gcgcaccatc gttgggccgc
    29101 cgatatcgat gttctcaacc gcatcttcca gcgagcagcc ttcacgggca acggtctggg
    29161 cgaacggata caggttaaca accaccatat cgataggctg gatctgatgt tcttccataa
    29221 tggcatcgtc ctggccgcga cggcccagaa ttccaccatg tactttcgga tgcagggtct
    29281 tcacgcgtcc atccatcatc tccgggaaac cggtgtaatc ggaaacttcg gttaccggca
    29341 gacctttttc tgctaacaga cgggcagtgc cccctgtaga cagcagctcc acaccgcgtg
    29401 cggaaagtgc ctgggcgaat tcgacgatac cggctttgtc agaaacactg agcagagcgc
    29461 ggcggactgg acgacgttgt tgcatggtaa atcccctgga tttgactatt acagagagcg
    29521 ttagctgaat ttttcgcgaa aaactcagct aacgccccta acggggcatc cttatttttc
    29581 gcccgcattg taacgaaaac gtttgcgcaa cgctcgcgaa tttttctctt tcaatggtga
    29641 tcacaatttt gacagtggct accgtgggaa aaatacagaa attacattga tgattgtgga
    29701 taactctgtg cgtaaaaagg tataaagtgg ggttttgctg gggaatgcag cagtcagtca
    29761 tttttatgca atttttctat tgcggcctgc ggag
//
LOCUS       DADSYM010000046        29666 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2415
            SAMN08039579-rid11723173.denovo.046, whole genome shotgun sequence.
ACCESSION   DADSYM010000046 DADSYM010000000
VERSION     DADSYM010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039579
            Sequence Read Archive: SRR6362533
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 29666)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29666)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980162.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 03:39:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,484
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 4,347
            CDSs (with protein)               :: 4,347
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 43 of 85
            Pseudo Genes (incomplete)         :: 37 of 85
            Pseudo Genes (internal stop)      :: 19 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29666
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039579-rid11723173.denovo.046"
                     /strain="LGE2415"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8302 N 95.9628 W"
                     /collection_date="2015-08-24"
     gene            43..300
                     /gene="bhsA"
                     /locus_tag="J1Y83_001729"
     CDS             43..300
                     /gene="bhsA"
                     /locus_tag="J1Y83_001729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple stress resistance protein BhsA"
                     /protein_id="HBA7629880.1"
                     /translation="MKNVKTLIAAAILSSMSFASFAAVEVQSTPEGQQKVGTISANAG
                     TNLGSLEEQLAQKADEMGAKSFRITSVTGPNTLHGTAVIYK"
     gene            complement(382..1344)
                     /gene="ldtC"
                     /locus_tag="J1Y83_001730"
     CDS             complement(382..1344)
                     /gene="ldtC"
                     /locus_tag="J1Y83_001730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase LdtC"
                     /protein_id="HBA7629881.1"
                     /translation="MMIKTRFSRWLTFFTFAAAVALALPAKANTWPLPSAGSRLVGEN
                     KFHVVENDGGSLEAIAKKYNVGFLALLQANPGVDPYVPRAGSVLTIPLQTLLPDAPRE
                     GIVINIAELRLYYYPPGKNSVTVYPIGIGQLGGDTLTPTMVTTVSDKRANPTWTPTAN
                     IRARYKAQGIELPAVVPAGPDNPMGHHAIRLAAYGGVYLLHGTNADFGIGMRVSSGCI
                     RLRDDDIKTLFSQVTPGTKVNIINTPIKVSAEPNGARLVEVHQPLSEKIDDDPQLLPI
                     TLNSAMQSFKDAAQTDAEVMQHVMDVRSGMPVDVRRHQVSPQTL"
     gene            complement(1487..4933)
                     /gene="mfd"
                     /locus_tag="J1Y83_001731"
     CDS             complement(1487..4933)
                     /gene="mfd"
                     /locus_tag="J1Y83_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HBA7629882.1"
                     /translation="MPEQYRYTLPVKAGEQRLLGELTGAACATLVAEIAERHAGPVVL
                     IAPDMQNALRLHDEISQFTDQMVMNLADWETLPYDSFSPHQDIISSRLSTLYQLPTMQ
                     RGVLIVPVNTLMQRVCPHSFLHGHALVMKKGQRLSRDALRTQLDSAGYRHVDQVMEHG
                     EYATRGALLDLFPMGSELPYRLDFFDDEIDSLRVFDVDSQRTLEEVDAINLLPAHEFP
                     TDKAAIELFRSQWRDTFEVKRDPEHIYQQVSKGTLPAGIEYWQPLFFSEPLPPLFSYF
                     PANTLLINTGDLENSAERFQADTLARFENRGVDPMRPLLPPQSLWLRVDELFSELKNW
                     PRVQLKTEHLPTKAANANLGFQKLPDLAIQAQQKAPLDALRKFLETFDGPVVFSVESE
                     GRREALGELLARIKIAPQRIMRLDEASDRGRYLMIGAAEHGFVDTMRNLALICESDLL
                     GERVARRRQDSRRAINPDTLIRNLAELHIGQPVVHLEHGVGRYAGMTTLEAGGITGEY
                     LMLTYANDAKLYVPVSSLHLISRYAGGAEENAPLHKLGGDAWSRARQKAAEKVRDVAA
                     ELLDIYAQRAAKEGFAFKHDREQYQLFCDSFPFETTPDQAQAINAVLSDMCQPLAMDR
                     LVCGDVGFGKTEVAMRAAFLAVDNHKQVAVLVPTTLLAQQHYDNFRDRFANWPVRIEM
                     ISRFRSAKEQTQILAEVAEGKIDILIGTHKLLQSDVKFKDLGLLIVDEEHRFGVRHKE
                     RIKAMRANVDILTLTATPIPRTLNMAMSGMRDLSIIATPPARRLAVKTFVREYDSLVV
                     REAILREILRGGQVYYLYNDVENIQKAAERLAELVPEARIAIGHGQMRERELERVMND
                     FHHQRFNVLVCTTIIETGIDIPTANTIIIERADHFGLAQLHQLRGRVGRSHHQAYAWL
                     LTPHPKAMTTDAQKRLEAIASLEDLGAGFALATHDLEIRGAGELLGEEQSGSMETIGF
                     SLYMELLENAVDALKAGREPSLEDLTSQQTEVELRMPSLLPDDFIPDVNTRLSFYKRI
                     ASAKTENELEEIKVELIDRFGLLPDPARTLLDIARLRQQAQKLGIRKLEGNEKGGVIE
                     FAEKNHVNPAWLIGLLQKQPQHYRLDGPTRLKFIQDLSERKTRIEWVRQFMRELEENA
                     IA"
     gene            complement(5061..6125)
                     /locus_tag="J1Y83_001732"
     CDS             complement(5061..6125)
                     /locus_tag="J1Y83_001732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HBA7629883.1"
                     /translation="MKQKELWINQIKGLCICLVVIYHSVITFYPHLTTFQHPLSEVLS
                     KCWIYFNLYLAPFRMPVFFFISGYLIRRYIDSVPWGNCLDKRIWNIFWVLALWGVVQW
                     LALSALNQWLAPERDLSNAAYADSTGEFLHGMITASTSLWYLYALIVYFVICKIFNRL
                     ALPLFVLFVLMSVAVNFVPTPWWGMNSVIRNLPYYSLGAWFGATLMTCVKAVPLRRHL
                     LMASLLTVLAVGAWLFTISLLLSLVSIVVIMKLFYQYEQRFGMRSSSLLNVIGSNTIA
                     IYTTHRILVEIFSLSLIPQMNAVRWSPQVELTLLLIYPFVSLLICTVAGLLVRKLSQR
                     AFSDLLFSPPSLPAAVSYSR"
     gene            6387..7586
                     /gene="lolC"
                     /locus_tag="J1Y83_001733"
     CDS             6387..7586
                     /gene="lolC"
                     /locus_tag="J1Y83_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit LolC"
                     /protein_id="HBA7629884.1"
                     /translation="MYQPVALFIGLRYMRGRAADRFGRFVSWLSTIGITLGVMALVTV
                     LSVMNGFERELQNNILGLMPQAILSSEHGSLNPQQLPETAVKLDGVNRVAPITTGDVV
                     LQSARSVAVGVMLGIDPAQKDPLTPYLVNVKQTDLEPGKYNVILGEQLASQLGVNRGD
                     QIRVMVPSASQFTPMGRIPSQRLFNVIGTFAANSEVDGYEMLVNIEDASRLMRYPAGN
                     ITGWRLWLDEPLKVDSLSQQKLPEGSKWQDWRDRKGELFQAVRMEKNMMGLLLSLIVA
                     VAAFNIITSLGLMVMEKQGEVAILQTQGLTPRQIMMVFMVQGASAGIIGAILGAALGA
                     LLASQLNNLMPIIGVLLDGAALPVAIEPLQVIVIALVAMAIALLSTLYPSWRAAATQP
                     AEALRYE"
     gene            7579..8280
                     /gene="lolD"
                     /locus_tag="J1Y83_001734"
     CDS             7579..8280
                     /gene="lolD"
                     /locus_tag="J1Y83_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309522.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter
                     ATP-binding protein LolD"
                     /protein_id="HBA7629885.1"
                     /translation="MNKILLQCDNLCKRYQEGSVQTDVLHNVSFSVGEGEMMAIVGSS
                     GSGKSTLLHLLGGLDTPTSGDVIFNGQPMSKLSSAAKAELRNQKLGFIYQFHHLLPDF
                     TALENVAMPLLIGKKKPAEINSRALEMLKAVGLEHRANHRPSELSGGERQRVAIARAL
                     VNNPRLVLADEPTGNLDARNADSIFQLLGELNRLQGTAFLVVTHDLQLAKRMNRQLEM
                     RDGRLTAELSLMGAE"
     gene            8280..9524
                     /gene="lolE"
                     /locus_tag="J1Y83_001735"
     CDS             8280..9524
                     /gene="lolE"
                     /locus_tag="J1Y83_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit LolE"
                     /protein_id="HBA7629886.1"
                     /translation="MAMPLSLLIGLRFSRGRRRGGMVSLISVISTIGIALGVAVLIVG
                     LSAMNGFERELNNRILAVVPHGEIEAVDQPWTNWQEALDNVQKVPGIAAAAPYINFTG
                     LVESGANLRAIQVKGVNPQQEQRLSALPSFVQGDAWRNFKAGEQQIIIGKGVADALKV
                     KQGDWVSIMIPNSNPEHKLMQPKRVRLHIAGILQLSGQLDHSFAMIPLADAQQYLDMG
                     SSVSGIALKMTDVFNANKLVRDAGEVTNSYVYIKSWIGTYGYMYRDIQMIRAIMYLAM
                     VLVIGVACFNIVSTLVMAVKDKSGDIAVLRTLGAKDGLIRAIFVWYGLLAGLFGSLCG
                     VIIGVVVSLQLTPIIEWIEKLIGHQFLSSDIYFIDFLPSELHWLDVFYVLVTALLLSL
                     LASWYPARRASNIDPARVLSGQ"
     gene            9553..10464
                     /gene="nagK"
                     /locus_tag="J1Y83_001736"
     CDS             9553..10464
                     /gene="nagK"
                     /locus_tag="J1Y83_001736"
                     /EC_number="2.7.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine kinase"
                     /protein_id="HBA7629887.1"
                     /translation="MYYGFDIGGTKIALGVFDSGRQLQWEKRVPTPRDSYDAFLDAVC
                     ELVAEADRRFGCKGSVGIGIPGMPETEDGTLYAANVPAASGKPLRADLSARLDRDVRL
                     DNDANCFALSEAWDDEFTQYPLVMGLILGTGVGGGLIFNGKPITGKSYITGEFGHMRL
                     PVDALTMMGLDFPLRRCGCGQHGCIENYLSGRGFAWLYQHYYHQPLQAPEIIALYDQG
                     DEQARAHVERYLDLLAVCLGNILTIVDPDLVVIGGGLSNFPAITTQLAERLPRHLLPV
                     ARVPRIERARHGDAGGMRGAAFLHLTD"
     gene            10480..11301
                     /gene="cobB"
                     /locus_tag="J1Y83_001737"
     CDS             10480..11301
                     /gene="cobB"
                     /locus_tag="J1Y83_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HBA7629888.1"
                     /translation="MLSRRGHRLSRFRKNKRRLRERLRQRIFFRDKVVPEAMEKPRVL
                     VLTGAGISAESGIRTFRAADGLWEEHRVEDVATPEGFDRDPELVQTFYNARRRQLQQP
                     EIQPNAAHLALAKLQDALGDRFLLVTQNIDNLHERAGNTNVIHMHGELLKVRCSQSGQ
                     VLDWTGDVTPEDKCHCCQFPAPLRPHVVWFGEMPLGMDEIYMALSMADIFIAIGTSGH
                     VYPAAGFVHEAKLHGAHTVELNLEPSQVGNEFAEKYYGPASQVVPEFVEKLLKGL"
     gene            complement(11427..12215)
                     /locus_tag="J1Y83_001738"
     CDS             complement(11427..12215)
                     /locus_tag="J1Y83_001738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HBA7629889.1"
                     /translation="MKKIMILLSLLMFLPLTAISKPLIPIMKTLFTDVTGTVPDAEEI
                     AHKAELFRQQTGVVPFIVVLPDINNEASLRQNGKAMLAHAASSMSNVKGSVLLLFTTR
                     EPRLIMITNGQVESSMDDKHLGLLVENHTLAYLHADLWYQGINNALAVLQAQILKQPT
                     PPLTYYPHPGQQHENDPPGSTTTLGLFAWAVAFIVFAAFFNYTTRLYYALKFAVAMAV
                     ANMGYQALCLYIDNSFAITRISPLWAGLIGVCTFIAALLWTSKR"
     gene            complement(12212..12673)
                     /locus_tag="J1Y83_001739"
     CDS             complement(12212..12673)
                     /locus_tag="J1Y83_001739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3592 domain-containing protein"
                     /protein_id="HBA7629890.1"
                     /translation="MSQDSRVFFRIFFGIGLVLILISVVIFYNQFTYSKDAIHTEGVI
                     VDTVWHSSHSHRTGKNGSWYPVVAFRPTPDYTLIFNSSIGSDFYEDSEGDKVNVYYSP
                     GHPEKAEINNPWVNFFKWGFIGIMGVIFIAVGLLISMPSSKKTRRKRKSRP"
     gene            complement(12731..13777)
                     /gene="potD"
                     /locus_tag="J1Y83_001740"
     CDS             complement(12731..13777)
                     /gene="potD"
                     /locus_tag="J1Y83_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein PotD"
                     /protein_id="HBA7629891.1"
                     /translation="MKKWSRHLLAAGALALGMSAAHADDNNTLYFYNWTEYVPPGLLE
                     QFTKETGIKVIYSTYESNETMYAKLKTYKDGAYDLVVPSTYYVDKMRKEGMIQKIDKS
                     KLSNFSNLDPDMLNKPFDPNNDYSIPYIWGATAIGVNGDAVDPKSVTSWADLWKPEYK
                     GSLLLTDDAREVFQMALRKLGYSGNTTDPKEIEAAYNELKKLMPNVAAFNSDNPANPY
                     MEGEVNLGMIWNGSAFVARQAGTPIDVVWPKEGGIFWMDSLAIPANAKNKEGALKLIN
                     FLLRPDVAKQVAETIGYPTPNLAARKLLSPEVANDKTLYPDAETIKNGEWQNDVGSAS
                     SIYEEYYQKLKAGR"
     gene            complement(13774..14568)
                     /gene="potC"
                     /locus_tag="J1Y83_001741"
     CDS             complement(13774..14568)
                     /gene="potC"
                     /locus_tag="J1Y83_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter permease
                     PotC"
                     /protein_id="HBA7629892.1"
                     /translation="MIGRLLRGGFMTAIYAYLYIPIIILIVNSFNSSRFGINWQGFTT
                     KWYSLLMNNDSLLQAAQHSLTMAVFSATFATLIGSLTAVALYRYRFRGKPFVSGMLFV
                     VMMSPDIVMAISLLVLFMLLGIQLGFWSLLFSHITFCLPFVVVTVYSRLKGFDVRMLE
                     AAKDLGASEFTILRKIILPLAMPAVAAGWVLSFTLSMDDVVVSSFVTGPSYEILPLKI
                     YSMVKVGVSPEVNALATILLVLSLVMVIASQLIARDKTKGNTGDVK"
     gene            complement(14565..15422)
                     /gene="potB"
                     /locus_tag="J1Y83_001742"
     CDS             complement(14565..15422)
                     /gene="potB"
                     /locus_tag="J1Y83_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000799378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter permease
                     PotB"
                     /protein_id="HBA7629893.1"
                     /translation="MKNTSKFQNVVIVTIVGWLVLFVFLPNLMIIGTSFLTRDDASFV
                     KMVFTLDNYTRLLDPLYFEVLLHSLNMALIATLACLVLGYPFAWFLAKLPHKVRPLLL
                     FLLIVPFWTNSLIRIYGLKIFLSTKGYLNEFLLWLGVIDTPIRIMFTPSAVIIGLVYI
                     LLPFMVMPLYSSIEKLDKPLLEAARDLGASKLQTFIRIIIPLTMPGIIAGCLLVMLPA
                     MGLFYVSDLMGGAKNLLIGNVIKVQFLNIRDWPFGAATSITLTIVMGLMLLVYWRASR
                     LLNKKVELE"
     gene            complement(15406..16542)
                     /gene="potA"
                     /locus_tag="J1Y83_001743"
     CDS             complement(15406..16542)
                     /gene="potA"
                     /locus_tag="J1Y83_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     ATP-binding protein PotA"
                     /protein_id="HBA7629894.1"
                     /translation="MGQSKKLNKQPSSLSPLVQLAGIRKCFDGKEVIPQLDLTINNGE
                     FLTLLGPSGCGKTTVLRLIAGLETVDSGRIMLDNEDITHVPAENRYVNTVFQSYALFP
                     HMTVFENVAFGLRMQKTPAAEITPRVMEALRMVQLETFAQRKPHQLSGGQQQRVAIAR
                     AVVNKPRLLLLDESLSALDYKLRKQMQNELKALQRKLGITFVFVTHDQEEALTMSDRI
                     VVMRDGRIEQDGTPREIYEEPKNLFVAGFIGEINMFNATVIERLDEQRVRANVEGREC
                     NIYVNFAVEPGQKLHVLLRPEDLRVEEINDDNHAEGLIGYVRERNYKGMTLESVVELE
                     NGKMVMVSEFFNEDDPDFDHSLDQKMAINWVESWEVVLADEEHK"
     gene            16792..18018
                     /gene="pepT"
                     /locus_tag="J1Y83_001744"
     CDS             16792..18018
                     /gene="pepT"
                     /locus_tag="J1Y83_001744"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HBA7629895.1"
                     /translation="MDKLLERFLNYVSLDTQSKAGVRQVPSTEGQWKLLHLLKEQLEE
                     MGLINVTLSEKGTLMATLPANVPGDIPAIGFISHVDTSPDCSGKNVNPQIVENYRGGD
                     IALGIGDEVLSPVMFPVLHQLLGQTLITTDGKTLLGADDKAGIAEIMTALAVLQQKNI
                     PHGDIRVAFTPDEEVGKGAKHFDVDAFDARWAYTVDGGGVGELEFENFNAASVNIKIV
                     GNNVHPGTAKGVMVNALSLAARIHAEVPADESPEMTEGYEGFYHLASMKGTVERADMH
                     YIIRDFDRKQFEARKRKMMEIAKKVGKGLHPDCYIELVIEDSYYNMREKVVEHPHILD
                     IAQQAMRDCDIEPELKPIRGGTDGAQLSFMGLPCPNLFTGGYNYHGKHEFVTLEGMEK
                     AVQVIVRIAELTAQRK"
     gene            complement(18067..19188)
                     /locus_tag="J1Y83_001745"
     CDS             complement(18067..19188)
                     /locus_tag="J1Y83_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309600.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HBA7629896.1"
                     /translation="MEYQLTLNWPDFLERHWQKRPVVLKRGFNNFIDPISPDELAGLA
                     MESEVDSRLVSHQDGKWQVSHGPFESYDHLGETNWSLLVQAVNHWHEPTAALMRPFRE
                     LPDWRIDDLMISFSVPGGGVGPHLDQYDVFIIQGTGRRRWRVGEKLHMRQHCPHPDLL
                     QVDPFEAIIDEELEPGDILYIPPGFPHEGYALENAMNYSVGFRAPNTRELISGFADYV
                     LQRELGGNYYSDPDVPPRAHPADVLPQEMDKLREMMLELINQPEHFKQWFGEFISQSR
                     HELDIAPPEPPYQPDEIYDALKQGDVLVRLGGLRVLRIGDDVYANGEKIDSPHRPALD
                     ALASNIALTAENFGDALEDPSFLAMLAALVNSGYWFFEG"
     gene            complement(19264..20724)
                     /gene="phoQ"
                     /locus_tag="J1Y83_001746"
     CDS             complement(19264..20724)
                     /gene="phoQ"
                     /locus_tag="J1Y83_001746"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     PhoQ"
                     /protein_id="HBA7629897.1"
                     /translation="MKKLLHLFFPLSLRVRFLLATAAVVLVLSLAYGMVALIGYSVSF
                     DKTTFRLLRGESNLFYTLAKWENNKLHVELPENIDKQSPTMTLIYDENGQLLWAQRDV
                     PWLMKMIQPDWLKSNGFHEIEADVNDTSLLLSGDHSIQQQLQEVREDDDDAEMTHSVA
                     VNVYPATSRMPKLTIVVVDTIPVELKSSYMVWSWFIYVLSANLLLVIPLLWVAAWWSL
                     RPIEALAKEVRELEEHNRELLNPATTRELTSLVRNLNRLLKSERERYDKYRTTLTDLT
                     HSLKTPLAVLQSTLRSLRSEKMSVSDAEPVMLEQISRISQQIGYYLHRASMRGGTLLS
                     RELHPVAPLLDNLTSALNKVYQRKGVNISLDISPEISFVGEQNDFVEVMGNVLDNACK
                     YCLEFVEISARQTDEHLYIVVEDDGPGIPLSKREVIFDRGQRVDTLRPGQGVGLAVAR
                     EITEQYEGKIVAGESMLGGARMEVIFGRQHSAPKDE"
     gene            complement(20724..21395)
                     /gene="phoP"
                     /locus_tag="J1Y83_001747"
     CDS             complement(20724..21395)
                     /gene="phoP"
                     /locus_tag="J1Y83_001747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator PhoP"
                     /protein_id="HBA7629898.1"
                     /translation="MRVLVVEDNALLRHHLKVQIQDAGHQVDDAEDAKEADYYLNEHL
                     PDIAIVDLGLPDEDGLSLIRRWRSNDVSLPILVLTARESWQDKVEVLSAGADDYVTKP
                     FHIEEVMARMQALMRRNSGLASQVISLPPFQVDLSRRELSINDEVIKLTAFEYTIMET
                     LIRNNGKVVSKDSLMLQLYPDAELRESHTIDVLMGRLRKKIQAQYPQEVITTVRGQGY
                     LFELR"
     gene            complement(21564..22934)
                     /gene="purB"
                     /locus_tag="J1Y83_001748"
     CDS             complement(21564..22934)
                     /gene="purB"
                     /locus_tag="J1Y83_001748"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006819529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HBA7629899.1"
                     /translation="MELSSLTAVSPVDGRYGDKVSALRGIFSEYGLLKFRVQVEVRWL
                     QKLAAHAAIKEVPAFAADAIGYLDAIVASFSEEDAARIKTIERTTNHDVKAVEYFLKE
                     KVADIPELHAVSEFIHFACTSEDINNLSHALMLKTARDEVILPYWRQLIDGIKDLAVQ
                     YRDIPLLSRTHGQPATPSTIGKEMANVAYRMERQYRQLNQVEILGKINGAVGNYNAHI
                     AAYPEVDWHQFSEEFVTSLGIQWNPYTTQIEPHDYIAELFDCVARFNTILIDFDRDVW
                     GYIALNHFKQKTIAGEIGSSTMPHKVNPIDFENSEGNLGLSNAVLQHLASKLPVSRWQ
                     RDLTDSTVLRNLGVGIGYALIAYQSTLKGVSKLEVNRDHLLEELDHNWEVLAEPIQTV
                     MRRYGIEKPYEKLKELTRGKRVDAEGMKQFIDGLELPEEEKARLKAMTPANYIGRAIT
                     MVDELK"
     gene            complement(22938..23579)
                     /gene="hflD"
                     /locus_tag="J1Y83_001749"
     CDS             complement(22938..23579)
                     /gene="hflD"
                     /locus_tag="J1Y83_001749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high frequency lysogenization protein HflD"
                     /protein_id="HBA7629900.1"
                     /translation="MAKNYYDITLALAGICQSARLVQQLAHQGHCDGDALHVSLNSII
                     DMNPSSTLAVFGGSEANLRVGLETLLGVLNASSRQGLNAELTRYTLSLMVLERKLSSA
                     KGALDTLGNRINGLQRQLEHFDLQSETLMSAMAAIYVDVISPLGPRIQVTGSPAVLQS
                     PQVQAKVRATLLAGIRAAVLWHQVGGGRLQLMFSRNRLTTQAKQILAHLTPEL"
     gene            complement(23615..24721)
                     /gene="mnmA"
                     /locus_tag="J1Y83_001750"
     CDS             complement(23615..24721)
                     /gene="mnmA"
                     /locus_tag="J1Y83_001750"
                     /EC_number="2.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309632.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HBA7629901.1"
                     /translation="MSETAKKVIVGMSGGVDSSVSAWLLQQQGYQVEGLFMKNWEEDD
                     GEEYCTAAADLADAQAVCDKLGIELHTVNFAAEYWDNVFELFLAEYKAGRTPNPDILC
                     NKEIKFKAFLEFAAEDLGADYIATGHYVRRADVDGKSRLLRGLDSNKDQSYFLYTLSH
                     EQIAQSLFPVGELEKPQVRKIAEDLGLVTAKKKDSTGICFIGERKFREFLGRYLPAQP
                     GKIITVDGDEIGEHQGLMYHTLGQRKGLGIGGTKDGTEEPWYVVDKDVENNILIVAQG
                     HEHPRLMSVGLIAQQLHWVDREPFTGTMRCTVKTRYRQTDIPCTVKALDADRIEVIFD
                     EPVAAVTPGQSAVFYNGEVCLGGGIIEQRLPLPV"
     gene            complement(24775..25236)
                     /gene="nudJ"
                     /locus_tag="J1Y83_001751"
     CDS             complement(24775..25236)
                     /gene="nudJ"
                     /locus_tag="J1Y83_001751"
                     /EC_number="3.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase NudJ"
                     /protein_id="HBA7629902.1"
                     /translation="MFKPHVTVACVVHAEGKFLVVEETINGKALWNQPAGHLEADETL
                     VEAAARELWEETGISAQPQHFIRMHQWIAPDKTPFLRFLFAIELEQICPTQPHDSDID
                     CCRWVSAEEILQASNLRSPLVAESIRCYQSGQRYPLEMIGDFNWPFTKGVI"
     gene            complement(25246..25899)
                     /gene="rluE"
                     /locus_tag="J1Y83_001752"
     CDS             complement(25246..25899)
                     /gene="rluE"
                     /locus_tag="J1Y83_001752"
                     /EC_number="5.4.99.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415653.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2457) synthase RluE"
                     /protein_id="HBA7629903.1"
                     /translation="MRQFIISENTMQKTSFRNHKVKRFSSQRSTRRKPENQPTRVILF
                     NKPYDVLPQFTDEAGRKTLKEFIPVQGVYAAGRLDRDSEGLLVLTNNGALQARLTQPG
                     KRTGKIYYVQVEGIPTQDALEALRNGVTLNDGPTLPAGVELVDEPAWLWPRNPPIRER
                     KSIPTSWLKITLYEGRNRQVRRMTAHVGFPTLRLIRYAMGDYSLDNLANGEWREVTD"
     gene            26071..27321
                     /gene="icd"
                     /locus_tag="J1Y83_001753"
     CDS             26071..27321
                     /gene="icd"
                     /locus_tag="J1Y83_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000444467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HBA7629904.1"
                     /translation="MESKVVVPAQGKKITLQNGKLNVPENPIIPYIEGDGIGVDVTPA
                     MLKVVDAAVEKAYKGERKISWMEIYTGEKSTQVYGQDVWLPAETLDLIREYRVAIKGP
                     LTTPVGGGIRSLNVALRQELDLYICLRPVRYYQGTPSPVKHPELTDMVIFRENSEDIY
                     AGIEWKADSADAEKVIKFLREEMGVKKIRFPEHCGIGIKPCSEEGTKRLVRAAIEYAI
                     ANDRDSVTLVHKGNIMKFTEGAFKDWGYQLAREEFGGELIDGGPWLKVKNPNTGKEIV
                     IKDVIADAFLQQILLRPAEYDVIACMNLNGDYISDALAAQVGGIGIAPGANIGDECAL
                     FEATHGTAPKYAGQDKVNPGSIILSAEMMLRHMGWTEAADLIVKGMEGAINAKTVTYD
                     FERLMEGAKLLKCSEFGDAIIKNM"
     gene            complement(27435..28577)
                     /locus_tag="J1Y83_001754"
     CDS             complement(27435..28577)
                     /locus_tag="J1Y83_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HBA7629905.1"
                     /translation="MAARPRSHKISIPNLYCKLDKRTGKVYWQYKHPLSGRFHSLGTD
                     ENEAKQVATEANTIIAEQRTRQILSVNERLERMKGRRSDITVTEWLDKYISIQEDRLQ
                     HNELRPNSYRQKGKPIRLFREHCGMQHLKDITALDIAEIIDAVKAEGHNRMAQVVRMV
                     LIDVFKEAQHAGHVPPGFNPAQATKQPRNRVNRQRLSLPEWQAIFDSVSRRQPYLKCG
                     MLLALVTGQRLGDICNLKFSDIWDDMLHITQEKTGSKLAIPLNLKCDALNITLREVIS
                     QCRDAVVSKYLVHYRHTTSQANRGDQVSANTLTTAFKKAREKCGIKWEQGTAPTFHEQ
                     RSLSERLYREQGLDTQKLLGHKSRKMTDRYNDDRGKDWVIVDMKTA"
     gene            complement(28567..28803)
                     /locus_tag="J1Y83_001755"
     CDS             complement(28567..28803)
                     /locus_tag="J1Y83_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HBA7629906.1"
                     /translation="MSRLITLQDWAKEEFGDLAPSERVLKKYAQGKMMAPPAIKVGRY
                     WMIDRNSRFVGTLAEPQLPINANPKLQRIIADGC"
     gene            complement(28943..29182)
                     /locus_tag="J1Y83_001756"
     CDS             complement(28943..29182)
                     /locus_tag="J1Y83_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016248605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HBA7629907.1"
                     /translation="MFRIIFPNTWYVDHHGTPCKILRSTHNKVHYIRKGRTCIASMFR
                     FNHDFEPVNKADADRIAEEIETAEHIKKLRAIRRK"
     gene            complement(29230..29448)
                     /locus_tag="J1Y83_001757"
     CDS             complement(29230..29448)
                     /locus_tag="J1Y83_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016246897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA family transcriptional regulator"
                     /protein_id="HBA7629908.1"
                     /translation="MADIIDSASEIEELQRNTAIKMRRLNHQAISATHCCECGDPIDE
                     RRRLAVQGCRTCASCQEEIELKNKQWGL"
BASE COUNT         7206 a         7438 c         7615 g         7407 t
ORIGIN      
        1 gtaattgctt gcgacgtcat tcatctgcat aaggccacta ttatgaaaaa cgtaaaaacc
       61 ctcatcgctg cggcgatttt aagctccatg tcatttgcca gctttgcggc tgtcgaagtt
      121 cagtccacgc cagaaggcca acaaaaagtc ggtactatca gtgctaacgc ggggacaaat
      181 ctgggatcgc tggaagagca gctggcgcaa aaagcggatg agatgggcgc aaaatctttc
      241 cgtattactt ctgtaaccgg tccgaatacc ctccatggaa cagcagtaat ttataaataa
      301 gcattaaccc tcattaatgc ctgctactgc tgattttttc cccgcgacat gctgtgtcgc
      361 ggggattttt ttatccgagc tttacagagt ttgtgggctc acttgatgac ggcggacatc
      421 caccggcatc ccggaacgga catccatcac atgttgcatc acttcagcgt cagtttgtgc
      481 tgcatcttta aatgattgca ttgcgctatt cagcgtaatt ggcagcagct gcggatcgtc
      541 atcaatcttc tctgacagcg gctgatgtac ttcaaccaga cgcgcaccgt ttggttcggc
      601 agagacttta atcggggtgt tgatgatatt cactttggtg cctggggtga cctggctaaa
      661 gagtgttttg atatcgtcat cccgcagacg aatacagcca gaacttaccc gcatgccaat
      721 gccgaaatca gcgttcgtac cgtgaagcaa ataaacgccg ccataggccg ccagacgaat
      781 cgcatgatgg cccattgggt tatccggtcc agccggaact accgcaggca actcaattcc
      841 ctgtgcctta taccgcgcgc gaatgtttgc cgttggcgtc caggttgggt ttgcacgctt
      901 gtcagaaacg gtggtcacca ttgtcggtgt cagcgtgtca ccacctaact gaccaatgcc
      961 gatgggatat acggttaccg aattttttcc cggcggataa taatagagac gcagctccgc
     1021 aatgttgatc acaatgcctt cgcgcggcgc gtctggaagt agggtttgca acgggatcgt
     1081 taacacgcta cccgcgcgcg gtacgtaagg atcaacgccg gggttagcct gtaacagagc
     1141 gagaaagccg acgttatatt ttttggcgat cgcttccaga gaaccaccgt cattttccac
     1201 cacatgaaat ttgttttcac caaccagacg actgcccgct gacggcagcg gccaggtgtt
     1261 ggcttttgcc ggtagcgcca gcgccacggc agcggcgaac gtaaaaaacg ttagccagcg
     1321 agaaaaacgc gttttgatca tcaccaaagt ccataataaa tataaggtta ttgtaataaa
     1381 aaggtaagtc tgaattatgg agggtgacag tgtcggataa tgcagggaag tgcaaagaat
     1441 ttgtaaatat tgcagatggg ggcgcagaaa cgcccccgat ttaccattaa gcgatcgcgt
     1501 tctcttccag ttcacgcata aactggcgta cccattcgat acgcgttttc cgctcgctca
     1561 aatcctgaat aaacttcaga cgcgtcggac catcaaggcg gtaatgctgc ggctgttttt
     1621 gcagcaaacc aatcaaccag gccggattaa cgtgattctt ctcggcaaat tcgatcacgc
     1681 cgcctttctc attaccttcc agcttcctga tccccagttt ctgcgcttgc tggcgcagac
     1741 gggcaatatc cagcagggta cgcgccggat ccggcagcag gccgaagcga tcgataagct
     1801 cgactttgat ctcttccagt tcgttttccg ttttggcgct ggcaatacgt ttgtagaacg
     1861 acagacgcgt gttcacgtca gggatgaaat catctggcaa tagcgacggc atccgcagct
     1921 cgacttctgt ttgctggctg gtgagatctt ccagcgacgg ctcgcgtccg gctttcagcg
     1981 catcgacggc gttttccagc aactccatat acagtgagaa accgatggtt tccattgagc
     2041 cgctttgttc ttcgccaagc agttcacctg cgccgcggat ctccagatcg tgcgttgcca
     2101 gcgcaaaacc tgcaccgaga tcttccagcg aggcaatcgc ttcaagacgt ttttgcgcat
     2161 cggtagtcat cgcttttgga tgcggcgtca gtagccatgc atatgcctgg tggtgagaac
     2221 gcccgacgcg accgcgcaac tggtgcaact gtgccagacc gaagtgatcc gcgcgttcaa
     2281 tgataatggt gttggcggta ggaatgtcga tcccggtttc gataatggtg gtacaaacca
     2341 gcacgttgaa acgttgatga tggaaatcat tcatcacccg ttccagttcg cgctcacgca
     2401 tttgcccgtg accgatggca atccgcgctt caggtaccag ttctgccagc cgttcggcgg
     2461 ctttctggat gttttccaca tcattgtaga gataataaac ctgtcctccg cgcaaaattt
     2521 cacgcaggat cgcctcccgg accaccagac tgtcatactc acggacaaag gttttaactg
     2581 ccagacgacg ggcgggcggc gtggcgataa tcgacagatc gcgcattccg ctcattgcca
     2641 tattcagcgt acgtgggatc ggcgttgcgg taagcgtcag aatatccacg ttcgcgcgca
     2701 tcgctttaat gcgctcttta tgacgcaccc cgaagcggtg ttcttcatcg acaatcagca
     2761 ggcctaaatc tttaaacttg acgtcacttt gcagcagttt gtgcgtaccg atcagaatat
     2821 cgattttccc ttccgccact tccgcaagga tttgcgtctg ctctttggcg ctgcggaaac
     2881 gggagatcat ttcgatacgt accggccagt tggcgaaacg gtcgcggaag ttgtcgtaat
     2941 gctgctgcgc gagaagggtg gtaggcacca gcaccgccac ctgtttgtgg ttatctaccg
     3001 ccaggaaagc ggcgcgcatc gccacttctg ttttaccaaa gccaacatcg ccgcacacca
     3061 gacgatccat tgccagcggc tgacacatgt cgctaagcac cgcattaatg gcttgagcct
     3121 gatccggcgt ggtttcaaac gggaagctgt cgcagaataa ctgatactgc tcacgatcgt
     3181 gtttaaacgc gaagccctct ttggcggcgc gttgcgcgta gatatccagc aattccgccg
     3241 ccacatcacg cactttttcc gccgctttct gccgtgcgcg tgaccaggca tcgccaccaa
     3301 gtttatgcag tggtgcgttt tcttctgcgc cacccgcgta acggctaatc agatgcagtg
     3361 acgacaccgg aacatacagt ttggcgtcgt tggcataggt gagcatcaaa tactcgccag
     3421 taatgcctcc agcttcgagc gtggtcattc cggcataacg accgacgccg tgctccagat
     3481 ggaccaccgg ctgaccaata tgcagctccg caaggttacg gatcagtgta tcggggttga
     3541 tggcgcggcg agaatcctga cgacgacggg caacgcgttc accgagcaga tcgctttcgc
     3601 aaatcagcgc caggttacgc atcgtatcga caaaaccatg ttcggcagcg ccaatcatca
     3661 gataacgccc acggtcgctg gcttcatcaa gacgcataat gcgttgcgga gcaattttaa
     3721 ttcgcgcgag cagttcaccc agcgcttcac ggcgaccttc actttctacc gagaacacca
     3781 ccggaccgtc gaaagtctcg aggaacttac gcagcgcatc cagcggcgct ttttgttgtg
     3841 cctgaatggc caggtctggc agtttctgga aacctaaatt ggcattcgct gcttttgtcg
     3901 gtaaatgttc agtttttagc tgcacacgcg gccagttttt cagctctgaa aagagctcgt
     3961 ctacccgcag ccagagcgat tgtggtggca acagtggacg catcggatcg acgccgcgat
     4021 tttcaaaacg cgccagcgtg tcagcctgga aacgttcggc actgttttcc agatcgccag
     4081 tatttatcag cagcgtattg gcagggaaat aactgaacag tggcggcagc ggttcgctga
     4141 agaatagcgg ctgccagtac tcgatcccgg caggtaatgt gcctttactc acttgctggt
     4201 aaatatgttc cggatctcgc ttcacttcga aggtatcgcg ccactggctg cggaacagtt
     4261 caattgccgc tttatcagtc ggaaattcgt gcgcgggcag cagattgatc gcatctactt
     4321 cctccagcgt gcgctggcta tcgacgtcaa acacccgcag gctgtcgatt tcatcatcaa
     4381 agaaatcaag acgataaggc agctcactcc ccatcgggaa gagatccagc aacgcgccgc
     4441 gcgtggcgta ttcgccgtgc tccatcacct ggtcaacatg gcgataaccg gcgctgtcca
     4501 gttgggttcg taatgcatct cgtgacaggc gctgaccttt tttcatcacc agcgcatgac
     4561 cgtggagaaa gctgtgcggg caaacgcgct gcataagcgt attcaccgga acaatcagta
     4621 cgccacgctg catggtcggt agctggtaaa gggtggaaag acgcgaggag ataatgtcct
     4681 gatgaggcga aaaactgtcg taagggaggg tttcccagtc cgccagattc atcaccatct
     4741 gatcggtgaa ctggctgatt tcatcatgca aacgcagagc attttgcata tctggtgcaa
     4801 tgagtaccac cggaccggcg tgacgttcgg caatttccgc taccagcgtc gcgcaggcag
     4861 cgccggttaa ctcgcccagc agacgctgct cacccgcttt gacgggcagc gtataacgat
     4921 attgttcagg cataacggtt gtcagattct cgttaggata tgcctcaaca tatgggggca
     4981 tatctctgat aagcaatgct gttcattatc cgtaatcgac atggtttagc aaattgaatc
     5041 gccccggctg gggcgatggc tcagcgggag taactgacgg cagcgggcag agaaggcggg
     5101 gagaacaaca gatcgctgaa tgcgcgctgt gaaagttttc ttaccagcaa gcccgcaaca
     5161 gtacaaatca acaaactgac aaaggggtaa atcagcagga gcgtcagttc gacttgcggc
     5221 gaccagcgta ccgcgttcat ttgcggaatc aggcttaagc tgaatatttc taccagaatg
     5281 cgatgggtgg tatagatagc aatggtgttg gagccaatca cattcagcag gctggaggaa
     5341 cgcataccga aacgctgctc gtactgataa aacagcttca tgattaccac aatcgatacc
     5401 agcgacaaca gcagcgagat agtaaacaac caggcaccga ccgccagaac ggtcagcaaa
     5461 gaagccatca gcagatggcg gcgcaacggt accgctttaa cacaggtcat taatgttgcg
     5521 ccaaaccatg cgccaaggct gtaataaggc aaattgcgga tcacactgtt cattccccac
     5581 cacggtgtgg gtacgaaatt aacagccaca ctcatcagta caaacagaac aaataacggc
     5641 agggccaggc ggttaaaaat tttacatatc acgaaataga caattaacgc atacagatac
     5701 cacaagctgg tgctggcggt gatcatcccg tgcaggaact caccggtgga atcggcataa
     5761 gcggcattgc ttaaatcgcg ctcaggtgcc agccactgat ttagtgcact tagcgccagc
     5821 cactgcacca cgccccaaag cgccagcacc cagaagatgt tccagatgcg tttatcgaga
     5881 caatttcccc acggtacgct gtcgatatag cggcgaatca aatagccaga gataaagaaa
     5941 aaaaccggca tacgaaaggg tgcaaggtaa agattgaaat agatccagca tttgctcagg
     6001 acttctgata acggatgctg gaaagtggtc agatgcggat aaaaggtaat gaccgagtga
     6061 taaatcacca ccagacaaat acataaccct ttgatctggt taatccatag ctctttttgt
     6121 ttcatcagga caacacgctc tttttattta acatgcggtt gatcctgcgg ggcgagtcgc
     6181 gtggtgtaat gcgtatctgt ctgtatttgg ttatttttca gataaagatc aggccacatg
     6241 gccgatgata aaatgaggca ctttagccga ttgatttgcc ggtacttttc agattagccc
     6301 tgacgatcac ttacagttca gacgtttacc catcccgctt tcgcttatat actcgtgtct
     6361 ttgctaacag caaccagacg gatttcatgt accaacctgt cgctctattt attggcctgc
     6421 gttacatgcg tgggcgtgca gcggatcgct tcggtcgttt cgtctcctgg ctttctacca
     6481 tcggcattac cctcggggtg atggcgctgg tcacagtatt gtcagtgatg aacggctttg
     6541 agcgcgagct gcaaaacaac atccttggcc tgatgccaca ggcaattctc tcttctgagc
     6601 atggctctct taacccgcag caactcccgg aaacggccgt caaactggac ggcgttaatc
     6661 gcgtcgcacc tattactacc ggtgatgtgg tactgcaaag cgcgcgcagc gtggcggtcg
     6721 gggtgatgct gggtatcgac ccggcgcaaa aagatccact aacgccgtat ctggtcaatg
     6781 tgaaacaaac tgacctcgag ccggggaaat ataatgtcat cctcggtgaa cagcttgcct
     6841 cacagttggg cgtcaatcgc ggtgatcaaa tccgcgtgat ggtgccatct gccagccagt
     6901 tcacgccgat ggggcgtatt ccaagtcagc gcctgttcaa tgtgattggc actttcgccg
     6961 ccaacagtga agtcgatggc tatgaaatgc tggtgaatat tgaggatgcc tcgcgtctga
     7021 tgcgttatcc ggcaggcaat attaccggct ggcgtttgtg gctggatgag ccgctgaaag
     7081 tcgactcatt aagtcagcaa aaactgcctg aaggcagcaa atggcaggac tggcgtgacc
     7141 gtaaaggcga gctgttccag gccgtacgca tggaaaaaaa tatgatgggc ttgctgctga
     7201 gcctgattgt cgccgttgcg gcgtttaaca ttattacctc gctgggactg atggtgatgg
     7261 agaagcaggg cgaagtggcc attcttcaga ctcaggggct gactccgcgt caaatcatga
     7321 tggtctttat ggtgcaaggg gccagcgctg ggattatcgg tgcgatcctc ggagcggcgc
     7381 ttggcgcact gcttgccagc cagttgaata atctgatgcc gataatcggc gtcctgcttg
     7441 atggcgcggc gctgccggtc gctatcgagc ctttacaggt cattgttatt gcgctggtgg
     7501 cgatggctat cgcgctgctg tctacgcttt acccttcctg gcgcgctgcc gccactcaac
     7561 ccgctgaggc tttacgttat gaataagatc ctgttgcaat gcgacaacct gtgcaaacgc
     7621 tatcaggaag gcagtgtgca aaccgatgta ctgcacaatg tcagtttcag cgtgggcgaa
     7681 ggtgaaatga tggcgatcgt cggcagctct ggttccggta aaagcacctt gctgcacctg
     7741 ttaggcgggc tggatacgcc aacctccggc gatgtgatct ttaacggtca accgatgagc
     7801 aaactgtcgt cggcggccaa agcggaactg cgtaaccaga agctgggatt tatttatcag
     7861 tttcaccacc tgttgccgga ttttaccgcc ctggaaaacg tggctatgcc gctgttgatt
     7921 ggcaagaaaa agcccgctga aatcaacagc cgtgcgctgg agatgttaaa agcggtgggg
     7981 ctggagcatc gtgcgaatca ccgcccatct gaactttctg gcggcgaacg ccagcgtgtg
     8041 gcgattgccc gtgcgctggt caataacccg cggctggtac tggcggatga acctactggt
     8101 aacctcgatg cacgtaacgc cgacagcatc ttccagttgc ttggggaatt gaatcgcttg
     8161 cagggcaccg ccttcctggt ggttactcac gacctgcaac tggcgaaacg tatgaaccgc
     8221 caactggaaa tgcgtgatgg acgtttgacg gcggaactga gcctgatggg ggcggagtaa
     8281 tggcgatgcc tttatcgtta ttgattggcc tgcgttttag ccgcggacgg cgacgcggcg
     8341 gcatggtgtc gttgatctcc gtcatttcta ccattggcat tgccctcggc gtggcggtat
     8401 tgatcgtcgg cttaagcgcg atgaacggct ttgaacgcga actgaataac cgcattctgg
     8461 cggtagtacc gcacggtgaa attgaggcgg tagatcagcc gtggaccaac tggcaggaag
     8521 cactggataa cgtgcagaaa gtgccgggca ttgccgccgc tgcgccgtat atcaatttca
     8581 ccgggctggt ggagagcggc gcgaatctgc gcgcaatcca ggtgaagggc gttaacccgc
     8641 aacaggaaca gcgtctgagc gcattaccct cgtttgttca gggggatgcc tggcgcaatt
     8701 ttaaagcggg cgaacagcaa attatcatcg gcaaaggcgt ggcggatgcg ctgaaagtga
     8761 agcagggcga ttgggtgtcg attatgatcc caaattcgaa tcccgagcat aaactgatgc
     8821 agccaaaacg tgtgcgtttg cacattgccg gtattttgca gttgagtggt caactcgatc
     8881 acagttttgc catgatcccg ctggccgatg ctcagcaata tcttgatatg ggttccagcg
     8941 tgtcaggtat tgcccttaaa atgacggatg ttttcaacgc caataagctg gtacgcgatg
     9001 cgggtgaagt gaccaacagc tatgtttata ttaaaagctg gattggtact tacggctata
     9061 tgtatcgcga tattcagatg atccgcgcca ttatgtatct ggcgatggta ctggtgattg
     9121 gcgtggcctg tttcaacatc gtctccacct tagtgatggc ggtgaaagac aagagtggcg
     9181 atatcgcagt attaagaacg ctgggggcga aagatggttt aattcgcgcc atctttgtct
     9241 ggtatggatt gctggcaggg ctgttcggca gcctgtgtgg ggtgattatc ggcgtagtgg
     9301 tttcactgca acttaccccg attattgagt ggattgaaaa gctgatcggt catcagttcc
     9361 tctccagcga tatctatttt attgacttct tgccatcgga attgcactgg ctggacgtct
     9421 tctacgtact ggtcacagca ttgttgctga gtcttttggc aagttggtat ccggcgcggc
     9481 gcgccagtaa tattgaccct gcgcgagtcc ttagcggcca gtaaaggcag tacattaaaa
     9541 caaggagcgg caatgtatta cgggtttgat attggtggaa caaaaattgc gctaggcgtg
     9601 tttgatagcg gtcggcagtt gcagtgggaa aagcgggtgc cgacaccgcg tgacagctat
     9661 gacgcatttt tagatgcagt gtgcgagctg gtagccgaag ctgaccggcg ttttggctgt
     9721 aaaggttctg tcggcatcgg tattccggga atgccggaaa cagaagatgg tacgctgtat
     9781 gccgccaatg tccctgctgc cagcggtaaa ccgctgcgtg ccgacctgag cgcacgtctt
     9841 gatcgcgatg tacgccttga taacgatgcc aactgttttg ccctttctga agcatgggat
     9901 gatgaattta ctcaatatcc actggtgatg gggttgattc tcggcaccgg cgttggcggc
     9961 gggctgattt tcaacggtaa acccattacc ggtaaaagct atattaccgg cgagtttggc
    10021 catatgcgtc tgccggttga tgcgttaacc atgatggggc tggatttccc gttacgccgc
    10081 tgcggctgtg gtcagcatgg ctgcattgaa aattatctgt ctggtcgcgg ttttgcgtgg
    10141 ctgtatcaac actattatca tcaaccgttg caggctcctg agatcattgc gctttatgat
    10201 caaggcgatg agcaggcaag ggcgcacgtt gagcgttatc tggatttatt agcggtttgt
    10261 ctgggaaata tcctgaccat tgttgaccct gacctggtcg tcattggtgg tggcttatcg
    10321 aatttcccgg caatcacaac gcaactggcg gaaaggttgc ctcgtcatct cttacctgta
    10381 gctcgtgttc cgcgcattga acgcgcgcgc cacggtgatg cggggggaat gcgtggtgcg
    10441 gccttcctac atctaaccga ttaatcaaca gaggttgcta tgctgtcgcg tcggggtcat
    10501 cggttaagtc gttttcgtaa aaataaacgc cgcctgcgcg agcgtttgcg tcagcgtatt
    10561 tttttcagag ataaagtggt gccggaagca atggaaaaac caagagtact cgtactgaca
    10621 ggggctggga tttctgcgga atcaggtatt cgtacctttc gcgccgcaga tggcctgtgg
    10681 gaagaacatc gggttgaaga tgtggcaacg ccggaaggtt ttgatcgcga tcctgaactg
    10741 gtgcaaacgt tttataacgc ccgtcgtcga cagttgcagc agccagaaat tcagcctaac
    10801 gctgcacatc ttgcgctggc gaaactgcaa gatgctctcg gcgatcgctt tttgctggtg
    10861 acgcagaata tcgacaatct gcatgaacgc gcaggcaata ccaatgtgat tcatatgcat
    10921 ggggaactgc tgaaagtacg ttgttcgcaa agtggccagg tcctcgactg gaccggagac
    10981 gttacgccag aagataaatg ccattgttgc cagttcccgg cacccttgcg cccgcacgta
    11041 gtgtggtttg gcgaaatgcc actcggcatg gatgaaattt atatggcgtt gtcgatggcc
    11101 gatattttca ttgctattgg tacttcgggg catgtttatc cggcggctgg gtttgttcac
    11161 gaagcgaaac tgcatggcgc gcacaccgtg gaactgaatc ttgaaccgag tcaggttggt
    11221 aatgaatttg ccgagaaata ttacggtccg gcaagccagg tggtgccaga gtttgttgaa
    11281 aagttgctga agggattata aagcgggaaa aattgcctga tgcgctacgc ttgacaggcc
    11341 tgtgaagggg cgaaattaat tgaatttgcg ggttttgtgg gccggataag gcgttcgcgc
    11401 cgcatccggc aactttactg cctcttttac cgtttactcg tccatagtaa cgcggcaata
    11461 aacgtacaga caccaatcag ccctgcccag agtggcgata ttctggtaat cgcaaagctg
    11521 ttatcgatat acaaacataa agcctgataa cccatattgg caacagccat cgccactgca
    11581 aattttaacg cataataaag gcgcgttgtg tagttaaaaa atgcagcaaa gacgatgaat
    11641 gcaactgccc aggcaaaaag gcctaatgtc gtggtactgc cgggtggatc attttcatgc
    11701 tgttgtccgg gatgcggata atatgtcagt ggtggcgtcg gttgttttaa tatctgtgct
    11761 tgtaaaacag ccaatgcatt attgataccc tgataccaga gatccgcatg taaataagcc
    11821 agcgtgtgat tttctaccag aaggccgaga tgtttatcgt ccatactgct ttcgacctgg
    11881 ccgttggtta tcataattaa ccgtggctcg cgggtagtaa agagtaacag aacacttcct
    11941 tttacattac tcattgaaga tgccgcatgc gccagcatcg ctttgccatt ttgcctgaga
    12001 ctggcttcat tattaatgtc gggtaaaacc acaataaacg gtacaactcc ggtttgttgg
    12061 cgaaaaagtt cggctttatg cgcgatctct tcagcatctg gtacagtacc ggtaacatcc
    12121 gtaaaaagtg tcttcattat tggaattaag ggcttactga ttgccgtcag aggcaggaac
    12181 atcagtaaac tgagcagtat catgattttt ttcatgggcg ggacttccgt ttcctgcgtg
    12241 tctttttaga ggagggcata ctgattaata aaccgacggc gataaaaata acgcccataa
    12301 tgccgataaa cccccattta aaaaaattaa cccacgggtt atttatctca gccttttcag
    12361 gatgtcctgg agaataataa acattaactt tatcgccttc gctgtcttca taaaaatcgc
    12421 tgccaatact cgaattgaaa attagcgtgt aatctggcgt cggacgaaag gcaacgacgg
    12481 gataccacga accattttta cccgtgcgat gagaatgact ggagtgccag acggtatcaa
    12541 caatgacgcc ttcagtatga atcgcgtctt tgctataggt gaactggtta taaaagataa
    12601 caacagaaat gaggatcagt accagaccga taccgaagaa tatacgaaag aaaactctac
    12661 tgtcctggct cattagccac atccttgcta aataaaaaac gggcggtaat accaccgccc
    12721 acttgctgaa ttaacgtcct gctttcagct tctgataata ctcttcataa atgctgctgg
    12781 cggagcctac gtcattttgc cattcaccat ttttaatggt ttcagcatcc gggtagagcg
    12841 ttttatcgtt cgccacttcc ggacttaaca gcttacgcgc cgcaaggttt ggcgttggat
    12901 aaccgatagt ttcagcaacc tgttttgcca catccgggcg cagcaggaag ttgatcaatt
    12961 tcagcgcgcc ttctttgttt ttggcatttg ccgggatcgc caggctgtcc atccagaaaa
    13021 tgccgccttc tttcggccat accacatcaa ttggcgtacc cgcctggcgt gcaacgaaag
    13081 cagaaccgtt ccagatcatg ccgaggttaa cttcgccttc catatacggg ttagcggggt
    13141 tatcggagtt aaacgccgcg acgtttggca tcagtttttt cagctcgttg tatgcagctt
    13201 caatctcttt cggatcggtg gtgttaccgg agtagcccag cttacgcagc gccatctgga
    13261 acacttcacg ggcatcgtcg gtcagcagca ggctgccttt gtactcaggc ttccacagat
    13321 cggcccagct ggtgacagat ttcggatcca ccgcatcacc gttaacgcca atcgccgttg
    13381 caccccagat atacggaatg gaatagtcgt tattcgggtc aaacggcttg ttgagcatgt
    13441 ctggatcgag gttgctgaaa ttgcttaact tcgacttgtc gatcttctgg atcatccctt
    13501 ctttacgcat tttatcgacg taataggttg aaggaaccac caggtcatag gcaccgtctt
    13561 tgtacgtttt cagcttcgcg tacatggttt cgttcgactc gtaagtcgaa tagataacct
    13621 taataccggt ttctttggtg aactgttcaa gcagtcctgg cggcacgtac tcggtccagt
    13681 tgtagaaata cagcgtgttg ttgtcatcgg cgtgagcggc gctcatgccc agtgccagag
    13741 cacccgccgc gagcaggtgg cgtgaccatt ttttcattta acgtcccctg tgttaccttt
    13801 cgttttatca cgagcaataa gctggctggc aattaccatc accagcgaca gcaccagtaa
    13861 tatggttgcc agcgcgttaa cttccggcga tacgccgact ttgaccatcg aataaatttt
    13921 taacggcaga atttcataac tcggcccggt gacaaacgaa gaaaccacca cgtcgtccat
    13981 cgacagggta aagcttaaca cccagcccgc cgctaccgct ggcattgcca gaggcagaat
    14041 gatttttcgc agaatggtaa attcgctggc accgagatct ttcgccgctt ccagcattcg
    14101 cacgtcaaaa cctttcaggc gcgaatacac tgtcaccacc acaaatggca ggcagaaggt
    14161 gatatgcgag aacagcagcg accagaagcc aagctgaata cccagcagca taaacagcac
    14221 cagcagcgaa atcgccatca cgatatctgg tgacatcatc accacaaaca gcattccgct
    14281 aacgaacggc ttaccacgaa agcgataacg gtacagcgca actgccgtca gtgaaccgat
    14341 aagcgtagca aacgtcgccg aaaacaccgc cattgttagt gaatgctgcg ctgcctgtaa
    14401 caggctgtcg ttgttcatca gcaggctata ccatttggtg gtaaaaccct gccagttgat
    14461 gccaaagcgc gagctgttaa aggagttcac aatcaaaata atgattggga tatacaggta
    14521 cgcgtagata gcggtcataa aaccgccgcg aagcagtcga ccgatcattc gagttccacc
    14581 ttcttattca gcagacgaga agcgcgccag taaaccagca acatcaggcc cattacgata
    14641 gtcagcgtaa tgctggtggc tgcaccaaac ggccagtcac gaatattgag gaactgtacc
    14701 ttgatgacgt taccgatcag caggtttttc gcaccgccca tcaggtcgga cacatagaac
    14761 aggcccatcg ctggcagcat caccagcaga catccggcaa taattcccgg catcgttagt
    14821 gggataatga tgcggataaa ggtctgcaac ttgctggcac cgagatcgcg cgccgcctca
    14881 agcaacggct tatccagttt ttcgatactg gagtacagcg gcatcaccat aaacggcagc
    14941 agaatgtaaa ccagaccgat aatcaccgca ctaggcgtga acatgatgcg aattggtgta
    15001 tcgataacgc ccagccagag caaaaactcg ttgagatagc ctttggtgct gaggaaaatt
    15061 ttcagcccgt aaatacgaat caatgagttg gtccagaacg gaacaatcag cagaaacagc
    15121 agcagcggac gcaccttgtg tggcagcttc gccagaaacc aggcaaaagg atagcccagc
    15181 accaggcagg cgagggtggc gatcagcgcc atattcagcg agtgcaatag cacttcaaaa
    15241 tagagcggat cgagcagacg cgtgtagtta tccagcgtaa agaccatttt gacgaaactg
    15301 gcgtcgtcgc gggtcaaaaa gctggtgcca atgatcatca ggttgggcag aaagacaaac
    15361 aacacaagcc aaccgacaat agtgacaatc actacattct ggaacttact tgtgttcttc
    15421 atcagccagt acgacctccc agctttctac ccaattaatg gccatttttt ggtcgagaga
    15481 gtggtcaaag tcaggatcgt cttcattgaa gaattcgctg accatcacca tcttgccatt
    15541 ttccagctca acaaccgact ccagcgtcat gcctttgtag ttacgctcgc gaacgtaacc
    15601 aatcagccct tcggcgtggt tgtcgtcgtt aatctcttca acacgtaagt cttccgggcg
    15661 cagcagaaca tgcagttttt gccccggttc aacggcgaag ttaacgtaga tattacattc
    15721 gcggccttca acgttggcgc gtacgcgctg ctcgtcgaga cgttcgatga cagtggcgtt
    15781 aaacatattg atttcgccaa taaacccggc aacaaacagg tttttcggct cttcgtagat
    15841 ttcacgcggc gtgccgtctt gctcaatgcg accatcgcgc atcaccacga tcctgtccga
    15901 catggtaagt gcttcttcct ggtcgtgagt tacgaacacg aatgtaatgc caagcttacg
    15961 ctgtaacgct ttcagctcgt tctgcatttg cttgcgcagt ttgtaatcca gcgctgagag
    16021 cgactcatcc agcaacaaca gacgaggctt gttaaccacc gcgcgagcaa tggcgacgcg
    16081 ttgctgttga ccaccagaga gctgatgcgg tttgcgttga gcgaaggttt ccagctgcac
    16141 catccgcagg gcttccatca cgcggggcgt aatttcagca gcgggggttt tttgcatgcg
    16201 caacccaaag gccacatttt cgaacacggt catgtggggg aaaagtgcgt agctttggaa
    16261 aacagtgttc acatagcggt tttccgccgg aacgtgggtg atgtcctcgt tatccagcat
    16321 gatgcgtccg gaatcaacag tttccagacc tgcaatcagg cgaagaacgg ttgttttacc
    16381 gcagccagaa gggccaagca gcgtgaggaa ctcgccattg ttgatagtca gatccagctg
    16441 gggaatgacc tctttaccat caaagcattt gcgaattccc gccaattgca ccagcggtga
    16501 aagcgaactc ggttgtttat tcaatttttt actctgtccc atgtaaacgc aacggatggc
    16561 ttaccgatgc ggggtttgtg gttaaccacc ttggtgactc ttaatgaggg cggtaattct
    16621 acggcaaacc gcttgaatcg ccaatctttg ttgtgaatta ctggcttagc tttatattca
    16681 ttaaggtaat gctgataaat attcccgctt gcagggctaa aagtgacctg acgcaatatt
    16741 tgtcttttct tgcttcttaa taatgttgtc acaaaaagtg agggtgacta catggataaa
    16801 ctacttgagc gatttttgaa ctacgtgtct ctggataccc aatcaaaggc aggggtgaga
    16861 caggttccca gcacggaagg ccaatggaag ttattgcatc tgctgaaaga gcagctcgaa
    16921 gagatggggc ttatcaatgt gaccttaagt gagaagggca ctttgatggc gacgttaccg
    16981 gctaacgtcc ctggcgatat cccggcgatt ggctttattt ctcatgtgga tacctcaccg
    17041 gattgcagcg gcaaaaatgt gaatccgcaa attgttgaaa actatcgcgg tggcgatatt
    17101 gcgctgggta tcggcgatga agttttatca ccggttatgt tcccggtgct gcatcagcta
    17161 ctgggtcaga cgctgattac caccgatggt aaaaccttgt taggtgccga tgacaaagca
    17221 ggtattgcag aaatcatgac cgcgctggcg gtattacaac aaaaaaacat tccgcatggt
    17281 gatattcgcg tcgcctttac cccggatgaa gaagtgggca aaggggcgaa acattttgat
    17341 gttgatgcct tcgatgcccg ctgggcttac accgttgatg gtggtggcgt aggtgaactg
    17401 gagtttgaaa acttcaacgc cgcgtcggtc aatatcaaaa ttgtcggtaa caatgttcac
    17461 ccgggcacgg cgaaaggagt aatggtaaat gcgctgtcgc tggcggcacg tattcatgcg
    17521 gaagttccgg cggatgaaag cccggaaatg acagaaggct atgaaggttt ctatcacctg
    17581 gcgagcatga aaggcaccgt tgaacgggcc gatatgcact acatcatccg tgatttcgac
    17641 cgtaaacagt ttgaagcgcg taaacgtaaa atgatggaga tcgccaaaaa agtgggcaaa
    17701 gggttacatc ctgattgcta cattgaactg gtgattgaag acagttacta caatatgcgc
    17761 gagaaagtgg ttgagcatcc gcatattctc gatatcgccc agcaggcgat gcgtgactgc
    17821 gatattgaac cggaactgaa accgatccgc ggcggtaccg acggcgcgca gttgtcgttt
    17881 atgggattac cgtgcccgaa cctgttcact ggcggttaca actatcatgg taagcatgag
    17941 tttgtgactc tggaaggtat ggaaaaagcg gtgcaggtga tcgtccgtat tgccgagttg
    18001 acggcgcaac ggaagtaagc gaaaagggat gcggcatgtg atgccgcatc cggcttaaat
    18061 ccaaacttac ccttcgaaga accagtatcc gctattgacc agcgccgcga gcatcgcgag
    18121 gaatgacgga tcttccagcg catcgccaaa attctccgcg gtcagcgcaa tgttgctggc
    18181 gagcgcatcc agtgccggac ggtgcgggga atcgatcttc tcaccattgg catacacgtc
    18241 gtcgccaatg cgcaatacgc gcagaccccc caggcgcacc agcacatcac cttgtttcag
    18301 cgcatcgtag atttcatccg gctgataagg cggctctggc ggcgcgatat ccagttcatg
    18361 acgtgactgg gatataaact cgccaaacca ttgtttaaag tgttccggct ggttgatcaa
    18421 ttcgagcatc atctcacgca gtttatccat ctcttgcggc agaacgtccg caggatgagc
    18481 gcgaggtgga acatcaggat cgctgtagta gttgccgccc agttcacgtt gcagcacata
    18541 atcggcaaat ccgctaatca gttcccgcgt atttggtgcg cgaaaaccca cggaatagtt
    18601 catcgcattt tccagcgcgt agccttcatg cgggaatcct ggcggaatat aaagaatatc
    18661 gcctggctcc agctcttcat caataatcgc ttcaaacgga tcaacctgta acagatccgg
    18721 gtgagggcag tgctgcctca tatgcagttt ttcacctacc cgccagcgac ggcgacctgt
    18781 accctgaata ataaacacat cgtactgatc gagatgcggg ccgacgccgc cgcccggtac
    18841 agaaaaagag atcatcagat catcaatacg ccagtccggt agttcacgga acggtcgcat
    18901 tagcgcagcg gtcggctcat gccagtggtt cactgcttgc actaacaatg accagttagt
    18961 ttcaccgaga tgatcgtagc tttcgaatgg accgtggctg acctgccatt ttccatcctg
    19021 atgactgacc agtcgactgt cgacttcgct ttccatcgcc agacccgcca actcgtctgg
    19081 agagattggg tcaataaaat tattaaagcc gcgttttaac accaccgggc gtttctgcca
    19141 gtgacgttca agaaaatcgg gccagttaag agtgagttgg tattccatgt taagcatcca
    19201 caggctggta tctgcaaccg attataacgg atgcttaacg taatgcgtga agtatggaca
    19261 tatttattca tctttcggcg cagaatgctg gcgaccaaaa atcacctcca tccgcgcacc
    19321 gcccagcatg ctctctccgg cgacgatttt accctcatat tgctcggtga tttcgcgggc
    19381 taccgccagc cccacacctt gcccagggcg taaagtatca acccgttgac cacggtcgaa
    19441 aatgacctct cgcttgctta atggaatacc ggggccatca tcctcgacca caatatagag
    19501 atgctcgtcg gtttgccttg cagaaatttc gacaaactcg aggcaatatt tacaggcgtt
    19561 atccagcacg ttgcccatca cttcgacaaa atcgttctgc tcaccaacaa agctgatttc
    19621 tggcgagata tcgagagaga tattgacccc tttgcgttga tacactttgt tcagcgccga
    19681 ggtgagattg tccaacagtg gcgcgaccgg atgcagctcg cggctaagca atgtcccgcc
    19741 gcgcatactg gcacgatgca ggtagtagcc aatttgctgt gaaatgcggc tgatttgctc
    19801 cagcattacc ggctcagcat cactgacgct catcttctca ctacgcagag aacgcagcgt
    19861 actttgcagc accgccagtg gcgttttcag actatgggtc aggtcggtga gcgttgtacg
    19921 atatttgtcg taacgttcgc gttcactttt taacagtcgg ttcaggtttc gtaccagact
    19981 ggtcagttct cgcgttgtgg ctggattgag caattcacgg ttatgttctt ccagttcgcg
    20041 gacttctttt gccagggctt cgatggggcg taaactccac caggcggcga cccacagcag
    20101 cgggatcact aacagcagat tggctgagag cacatagata aaccagctcc agaccatata
    20161 ggaacttttt agctccaccg gaatggtatc caccaccaca atggttaact ttggcatccg
    20221 cgatgttgcc gggtagacgt ttaccgccac cgagtgggtc atctccgcgt cgtcatcatc
    20281 ttcccgcact tcctgcaact gttgctgtat cgaatgatct ccactcagca agaggctggt
    20341 atcgttaaca tccgcttcaa tttcatgaaa accattcgat ttcagccagt caggctggat
    20401 catcttcatc agccagggca cgtcacgttg cgcccataaa agctgcccgt tctcatcata
    20461 aattagtgtc atcgtggggc tttgcttgtc gatattttcg ggtaactcga catgcaactt
    20521 attgttttcc cacttcgcaa gggtatagaa cagattgctc tcgccacgta acagccgaaa
    20581 cgtggtttta tcgaaactga cgctataacc gatcagcgca accattccgt aggcaagcga
    20641 aagcaccaat actactgctg ccgttgctaa cagaaaacgt acccgcagcg agagcgggaa
    20701 aaaaagatgc agtaattttt tcatcagcgc aattcgaaca gatagccctg gccgcgaacg
    20761 gtggtaatca cttcttgcgg atattgtgcc tgtatttttt tgcgcagacg tcccatcagt
    20821 acatctatgg tatgactttc ccgcagctcc gcatccggat agagttggag cattaacgaa
    20881 tctttgctga ccactttgcc attattgcgt atcaacgttt ccataatggt gtattcgaac
    20941 gcggtcagtt tgatcacttc gtcattaata gataattcac ggcgggagag atcaacctga
    21001 aacggtggta gcgaaatgac ctgtgaagcc agaccgctat tacgccgcat taatgcctgc
    21061 attcgcgcca tcacctcttc aatatgaaac ggtttagtca cgtaatcatc agcaccggca
    21121 cttaatactt cgactttgtc ctgccagctc tcacgggcgg ttaataccag aatcggcagt
    21181 gaaacatcat tgctacgcca gcggcgaatc agtgacagac catcctcgtc aggcaatccg
    21241 agatcgacaa tcgcaatatc cggtaaatgt tcattgagat aataatcggc ttctttggca
    21301 tcttctgcgt catcgacctg atgaccagca tcctgaatct gaactttaag atggtgacgt
    21361 aacaacgcat tgtcttcaac aaccagtacg cgcattttta tttctccctg tcttaattat
    21421 taaaatagtg taacgcgatt atgtggttat gggggtaaac attaaataaa ccagcaggaa
    21481 ggggaggtaa agtgaaaaaa caaaaagtgg ataatcttaa taagcaggcc ggacagcatc
    21541 gccatccggc actgatttga ggcttatttc agctcatcaa ccatggtgat ggcgcgacca
    21601 atatagttag ccggcgtcat cgctttcagg cgggctttct cttcttctgg caactccaga
    21661 ccgtcgataa attgcttcat gccttcggcg tcaacgcgct taccgcgagt cagctctttc
    21721 agcttctcgt acggtttttc gatgccatag cgacgcataa ctgtctggat tggctcagcc
    21781 agcacttccc agttgtgatc cagctcctcc agcagatggt cacggttcac ttccagtttg
    21841 ctcacgcctt tcagggtgga ttgatacgca atcagcgcat aaccgatacc cacgccgagg
    21901 ttacgcagca cggtggagtc ggtcaggtca cgctgccagc gggaaactgg cagtttgctt
    21961 gccaggtgct gcaataccgc gttggaaagg cccaggttcc cttcggagtt ttcgaagtcg
    22021 atcgggttaa ctttatgcgg catggtggaa gaaccaatct caccagcaat ggttttctgt
    22081 ttaaagtggt taagggcgat ataaccccag acgtcacggt caaagtcgat cagaatggtg
    22141 ttgaagcgcg caacgcaatc aaacagttcg gcaatgtagt cgtgcggttc gatctgggtg
    22201 gtgtacgggt tccactgaat acccagcgag gtgacgaact cttcgctgaa ctgatgccag
    22261 tcaacttccg ggtatgcggc gatgtgggca ttatagttgc cgacagcgcc gttgattttg
    22321 ccgaggatct ccacctgatt aagctggcgg tactggcgct ccatacggta ggcgacgtta
    22381 gccatctctt taccgatggt tgacggcgtg gctggctgac cgtgggtacg agacagcagc
    22441 gggatatcgc gatactgaac ggcgagatct ttaatgccat caatcagttg acgccagtac
    22501 ggcaggatca cttcatcacg cgcggttttc agcattaatg cgtgggagag gttattgata
    22561 tcttccgaag tacaggcaaa gtggatgaat tcagaaaccg cgtgcagttc cgggatatcc
    22621 gccacttttt ctttcaggaa atactcaacc gctttaacgt cgtggttagt ggtacgctcg
    22681 atggttttga tgcgtgctgc atcttcttcg ctgaaactgg cgacaattgc atcaaggtaa
    22741 ccgattgcgt cggcagcaaa agcaggaact tccttgatcg ctgcgtgcgc ggccagtttt
    22801 tgcagccaac gtacttcaac ttgtacacgg aatttcagca aaccgtattc gctgaaaatc
    22861 ccgcgcagcg cgctgacttt atcgccgtag cgtccatcga caggggaaac ggcggtcagt
    22921 gaggataatt ccatagatca caactccggg gttaaatgag caagaatttg ttttgcctga
    22981 gtggtcaggc gattacgaga aaacatcagt tgcagacgac cgccgccgac ctggtgccag
    23041 agcacggcgg cgcgaatgcc tgccagcagg gttgcgcgaa ctttcgcctg cacctgtggg
    23101 ctttgcagta cagctgggga accagtgacc tgaatgcgcg ggccaagcgg gctgataaca
    23161 tcaacataga tggcagccat cgcgctcatc agcgtttcgg actgtaaatc gaagtgttcg
    23221 agctggcgtt gcaggccgtt gatccggttg cccagagtgt cgagcgcacc tttcgctgag
    23281 gagagtttgc gctcaagcac catcagactg agtgtgtagc gggttaattc ggcgtttaag
    23341 ccctggcgac tgctggcatt gagtacgccg agcaaggttt ccagcccgac gcgcaggttg
    23401 gcttcgctac cgccaaaaac cgccagcgtt gagctggggt tcatatcgat aatactgttg
    23461 agtgagacgt gtagcgcatc gccatcacaa tgcccctggt gagcgagttg ttgcaccagg
    23521 cgtgccgact gacaaatacc ggcaagggcg agggtgatgt catagtaatt ctttgccacg
    23581 ttcactgctt ccttgtttta agtaaagata ataatcagac cggcagcggc agacgctgct
    23641 caataatacc gccaccgagg cacacttcac cgttatagaa aacggcagac tggcccggcg
    23701 tcacggcggc aaccggctca tcgaaaatca cttcaatgcg atcagcgtcc agcgccttga
    23761 cggtgcacgg gatgtcggtc tggcgatagc gggtttttac tgtgcaacgc atagtaccgg
    23821 tgaacggctc gcgatcgacc cagtgcagct gctgggcaat cagaccgaca gacatcagac
    23881 gcgggtgttc atggccttgc gcaacaatca gaatgttgtt ttcgacgtct ttatcgacca
    23941 cataccacgg ttcttcggtg ccgtctttgg tgccaccgat acccagacct ttacgctgac
    24001 cgagggtgtg atacatcagc ccctggtgct caccaatttc atcgccatcg acggtaatga
    24061 ttttgcccgg ttgcgccggg agataacggc ccaggaactc gcggaattta cgttcgccaa
    24121 tgaagcaaat gccggtagag tcttttttct tcgcggtgac cagaccaaga tcttcagcaa
    24181 tcttacgcac ctgcggtttt tccagttcgc cgaccgggaa caggctttgc gcaatctgct
    24241 catggctgag cgtataaagg aagtagctct ggtctttatt gctgtccaga ccacgcagca
    24301 ggcggctctt gccatcgaca tctgcccgac gtacgtaatg accggtagcg atataatcgg
    24361 cacctaaatc ttcggcggca aattcgagga aggctttaaa tttgatctct ttgttgcaca
    24421 gaatatccgg attcggcgtg cgaccggctt tatattcggc aaggaacagt tcgaagacgt
    24481 tgtcccagta ctcggcagca aagttaacgg tgtgcagttc aatgccgagc ttgtcgcaga
    24541 cagcctgggc atcagccaga tccgctgccg ctgtgcaata ttcctcaccg tcgtcttctt
    24601 cccagttctt cataaacagg ccttcgacct gatatccctg ttgttgcaac agccaggcag
    24661 aaacggagga atcgacaccg ccggacatgc cgacgattac tttttttgcg gtttcagaca
    24721 ttggatcact cacgacattg aacttcaagg cggcgtattc tatcacgcag ccttttagat
    24781 gacacccttt gtaaaaggcc agttaaaatc gccaatcatc tccagcggat agcgttgccc
    24841 gctttgataa caacgaatac tttccgccac cagcggcgaa cgcagatttg acgcctgtaa
    24901 aatttcttcg gcgctgaccc aacggcagca gtcgatatcg ctgtcatgag gctgggtcgg
    24961 gcatatttgc tcaagctcaa tagcaaacag gaaacgcaaa aacggcgttt tatctggcgc
    25021 aatccactga tgcatacgaa taaagtgttg cggctgcgcg ctgatgccgg tttcttccca
    25081 cagctcacgg gcggcggctt ccactaaggt ttcatcggct tccagatgcc cggcaggttg
    25141 gttccataac gctttaccat taatcgtctc ttcaacgact aaaaatttgc cttctgcgtg
    25201 caccacgcaa gcaacggtaa cgtgcggttt aaacattttg catccttaat ctgtcacttc
    25261 tcgccattcg ccattggcaa gattatccaa agagtaatca cccatcgcat agcgaatcag
    25321 tcgcagcgtg gggaaaccaa catgggcggt catgcggcgc acctggcgat tacgtccttc
    25381 atataaggtg atcttcagcc agctggtggg aatacttttg cgttcacgaa ttggtggatt
    25441 ccgcggccat aaccacgcgg gttcgtcaac cagctctacg cctgcgggca gggtagggcc
    25501 atcatttaag gttacgccat tgcgcaaggc ttcaagtgcg tcttgtgtgg gaataccttc
    25561 cacctgcaca taatagattt ttccggtgcg tttacccggc tgggttaaac gcgcctgcaa
    25621 cgcgccgtta ttggtcagca ccagtaaccc ttcgctatcg cggtcaaggc gacctgctgc
    25681 ataaacgcct tgaaccggga tgaattcttt taatgttttg cgtccggctt catcggtgaa
    25741 ctgcggaaga acatcgtagg gtttattgaa caggatcaca cgtgtgggct ggttttcagg
    25801 tttacgcctg gtagaacgtt gcgagctgaa tcgcttaacc ttgtgatttc taaaagaagt
    25861 tttttgcatg gtattttcag agattatgaa ttgccgcatt atagcctaat aacgcgcatc
    25921 tttcatgacg gcaaacaata gggtagtatt gacaagccaa ttacaaatca ttaacaaaaa
    25981 attgctctaa agcatccgta tcgcaggacg caaacgcata tgcaacgtgg tggcagacga
    26041 gcaaaccagt agcgctcgaa ggagaggtga atggaaagta aagtagttgt tccggcacaa
    26101 ggcaagaaga tcaccctgca aaacggcaaa ctcaacgttc ctgaaaatcc gattatccct
    26161 tacattgaag gtgatggaat cggtgtagat gtaaccccag ccatgctgaa agtggtcgac
    26221 gctgcagtcg agaaagccta taaaggcgag cgtaaaatct cctggatgga aatttacacc
    26281 ggtgaaaaat ccacacaggt ttatggtcag gatgtctggc tgcctgctga aacccttgat
    26341 ctgattcgtg aatatcgcgt tgccattaaa ggcccgctga ccactccggt tggtggcggt
    26401 attcgctctc tgaacgttgc cctgcgccag gaactggatc tctacatctg cctgcgtccg
    26461 gtacgttact accagggcac cccaagcccg gttaaacacc ctgaactgac cgatatggtt
    26521 atcttccgtg aaaactcgga agacatttat gcgggtatcg aatggaaagc agactctgcc
    26581 gacgccgaga aagtgattaa attcctgcgt gaagagatgg gcgtgaagaa aattcgtttc
    26641 ccggaacatt gcggtatcgg tattaagccg tgttctgaag aaggcaccaa acgtctggtg
    26701 cgtgcagcga tcgaatacgc aattgcgaac gatcgtgact ctgtgaccct ggtgcacaaa
    26761 ggcaacatca tgaagttcac cgaaggcgcg tttaaagact ggggctacca gctggcgcgt
    26821 gaagagtttg gcggtgaact gatcgacggc ggcccgtggc tgaaagttaa aaacccgaat
    26881 accggcaaag agatcgtcat taaagatgtg attgctgatg cattcctgca acagatcctg
    26941 ctgcgtccgg ctgaatatga tgttatcgcc tgtatgaacc tgaacggtga ctacatttct
    27001 gacgctctgg ctgcacaggt cggcggtatc ggtatcgccc caggcgcaaa catcggtgac
    27061 gaatgcgccc tgtttgaagc cacccacggt actgcgccga aatatgccgg tcaggacaaa
    27121 gttaaccctg gctctattat tctctccgct gagatgatgt tacgccatat gggctggact
    27181 gaagccgcag acttgattgt taaaggtatg gaaggcgcaa tcaacgccaa gaccgtaacc
    27241 tatgacttcg aacgtctgat ggaaggtgct aaactgctga aatgcagcga gtttggtgat
    27301 gcaatcatca agaacatgta atctctacat gtgttaaata ttgaaacggg cgtataacac
    27361 gcccgttgtt ttatgatgat gtaaaatctt ccccaaaact ttccccaaaa cccttcccca
    27421 aaactggcta ttttctatgc tgttttcata tctacgataa cccagtcttt accacgatca
    27481 tcattgtatc ggtcggtcat ttttctggat ttatgaccta acaacttttg cgtatccaga
    27541 ccctgttccc gatataaccg ttctgacaga gatcgctgct catgaaatgt gggcgcagtt
    27601 ccttgctccc attttatgcc acatttttcc ctggcctttt taaaagccgt tgtcagagta
    27661 tttgcagaaa cctggtcccc tctgtttgct tgagaggttg tgtgacggta atggaccaga
    27721 tatttactaa caacagcatc cctgcactga gatataactt cacgaagggt aatattcaga
    27781 gcatcgcatt tcaggttaag cggaatagca agttttgaac cggttttttc ctgagtaatg
    27841 tgcaacatgt cgtcccagat atcagagaat ttcaaattgc agatatcgcc taaacgttgt
    27901 ccagtgacaa gagcaagtag catcccgcat tttaaatagg gctgccgtct gcttacgctg
    27961 tcaaatattg cctgccattc gggcagtgat aatctttggc ggtttactcg atttcgcggt
    28021 tgttttgttg cctgcgctgg gttaaatcct ggcggaacat gtcctgcgtg ttgtgcttct
    28081 ttgaagacgt cgatcaacac cattctcacg acttgcgcca tcctgttatg accttcagcc
    28141 tttacagcat caattatttc ggcaatatca agtgcggtaa tatccttgag gtgttgcatt
    28201 ccacaatgct cacggaaaag acgaatgggt ttgccttttt gccgatagga gttgggtctt
    28261 agttcattat gttgcagcct gtcctcctgg atagaaatat atttatcaag ccattctgtc
    28321 accgtaatgt ctgagcgcct gcctttcatt ctttctagac gctcattgac gcttaatatt
    28381 tgtcgagtac gttgttcagc aataatggta tttgcttcag tagcaacttg ttttgcttca
    28441 ttctcatcag ttcctaagct atgaaaacga ccggatagtg gatgtttgta ttgccaatat
    28501 acctttccgg ttcgcttatc taatttgcaa tataaattgg gtatagagat tttgtgagat
    28561 cggggtctag cagccatcag cgattatccg ttggagtttt gggtttgcgt ttattgggag
    28621 ttgcggttct gcaagcgttc ctacaaaacg ggaatttcgg tcaatcatcc agtagcgacc
    28681 aacttttata gcgggtgggg ccatcatttt cccttgcgcg tattttttca gaactcgctc
    28741 acttggtgct aagtccccaa attcttcttt agcccagtcc tgtaaagtga ttagtcgaga
    28801 catttgtcct cctcttagct gctgagggag tttgtgaccg atatatctga catgatatta
    28861 agctcatggc aggtacatct cttgactggt catagagata aatttaatgc tgagaaatgc
    28921 agtattgaat ttatcaattt ttctatttcc tgcgtatggc acgtaacttc ttaatgtgtt
    28981 ctgccgtttc gatctcttct gctatccgat ctgcatcagc tttattcaca ggttcaaagt
    29041 catgattaaa gcggaacatg ctggcgatac atgttctgcc ttttcggatg tagtgaactt
    29101 tgttgtgggt agaacgcagg attttgcagg gagtgccgtg gtggtcgacg taccaggtgt
    29161 taggaaaaat gattctgaac atttttacac ctcagttgga cgatgttgaa atttgctgct
    29221 ttgaggccat cacagtcccc attgtttgtt cttaagttcg atctcctcct ggcaacttgc
    29281 acaagtccga caaccctgaa cggccaggcg tcttcgttca tctatcggat cgccacactc
    29341 acaacaatga gtggcagata tagcctggtg gttcaggcgg cgcattttta ttgctgtgtt
    29401 gcgctgtaat tcttcaattt ctgatgctga atcaatgatg tctgccatct ttcattaatc
    29461 cctgaattgt tggttaatac gcttgagggt gaatgcgaat aataaaaaag gagcctgtag
    29521 ctccatgatg attttgtttt tcatgttcac cgttccttaa agacgccgtt taacatgccg
    29581 atcgccaggc ttaaatgagt cggtgtgaat cccatcagcg ttaccgtttc gcggtgcttc
    29641 ttcagtacgc tacggcaaat gtcatc
//
LOCUS       DADSYM010000047        29661 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2415
            SAMN08039579-rid11723173.denovo.047, whole genome shotgun sequence.
ACCESSION   DADSYM010000047 DADSYM010000000
VERSION     DADSYM010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039579
            Sequence Read Archive: SRR6362533
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 29661)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29661)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980162.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 03:39:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,484
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 4,347
            CDSs (with protein)               :: 4,347
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 43 of 85
            Pseudo Genes (incomplete)         :: 37 of 85
            Pseudo Genes (internal stop)      :: 19 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29661
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039579-rid11723173.denovo.047"
                     /strain="LGE2415"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8302 N 95.9628 W"
                     /collection_date="2015-08-24"
     gene            complement(26..1693)
                     /gene="ettA"
                     /locus_tag="J1Y83_001758"
     CDS             complement(26..1693)
                     /gene="ettA"
                     /locus_tag="J1Y83_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HBA7629909.1"
                     /translation="MAQFVYTMHRVGKVVPPKRHILKNISLSFFPGAKIGVLGLNGAG
                     KSTLLRIMAGIDKDIEGEARPQPDIKIGYLPQEPQLNPEHTVRESIEEAVSEVVNALK
                     RLDEVYALYADPDADFDKLAAEQGRLEEIIQAHDGHNLNVQLERAADALRLPDWDAKI
                     ANLSGGERRRVALCRLLLEKPDMLLLDEPTNHLDAESVAWLERFLHDFEGTVVAITHD
                     RYFLDNVAGWILELDRGEGIPWEGNYSSWLEQKDQRLAQEASQEAARRKSIEKELEWV
                     RQGTKGRQSKGKARLARFEELNSTEYQKRNETNELFIPPGPRLGDKVLEVSNLRKSYG
                     DRLLIDSLSFSIPKGAIVGIIGPNGAGKSTLFRMISGQEQPDSGTITLGETVKLASVD
                     QFRDSMDNSKTVWEEVSGGLDIMKIGNTEMPSRAYVGRFNFKGVDQGKRVGELSGGER
                     GRLHLAKLLQVGGNMLLLDEPTNDLDIETLRALENALLEFPGCAMVISHDRWFLDRIA
                     THILDYQDEGKVEFFEGNFTEYEEYKKRTLGADALEPKRIKYKRIAK"
     gene            1900..3837
                     /gene="sltY"
                     /locus_tag="J1Y83_001759"
     CDS             1900..3837
                     /gene="sltY"
                     /locus_tag="J1Y83_001759"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase"
                     /protein_id="HBA7629910.1"
                     /translation="MEKAKHVTWRLLAVGVCLLTVSSVARADSLDEQRSRYAQIKQAW
                     DNRQMDVVEQMMPGLKDYPLYPYLEYRQITDDLMNQPAVTVTNFVRANPTLPPARTLQ
                     SRFVNELARREDWRGLLAFSPEKPGTTEAQCNYYYAKWNTGQSEEAWQGAKELWLTGK
                     SQPNACDKLFSVWRASGKQDPLAYLERIRLAMKAGNTGLVTVLAGQMPADYQTIASAI
                     ISLANNPNTVLTFARTTGATDFTRQMAAVAFASVARQDAENARLMIPSLAQAQQLNED
                     QIQELRDIVAWRLMGNDVTDEQAKWRDDAIMRSQSTSLIERRVRMALGTGDRRGLNTW
                     LARLPMEAKEKDEWRYWQADLLLERGREAEAKEILHQLMQQRGFYPMVAAQRIGEEYE
                     LKIDKAPQNVDSALTQGPEMARVRELMYWNLDNTARSEWANLVKSKSKTEQAQLARYA
                     FNNQWWDLSVQATIAGKLWDHLEERFPLAYNDLFKRYTSGKEIPQSYAMAIARQESAW
                     NPKVKSPVGASGLMQIMPGTATHTVKMFSIPGYSSPGQLLDPETNINIGTSYLQYVYQ
                     QFGNNRIFSSAAYNAGPGRVRTWLGNSAGRIDAVAFVESIPFSETRGYVKNVLAYDAY
                     YRYFMGDKPTLMSATEWGRRY"
     gene            3927..4253
                     /gene="trpR"
                     /locus_tag="J1Y83_001760"
     CDS             3927..4253
                     /gene="trpR"
                     /locus_tag="J1Y83_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trp operon repressor"
                     /protein_id="HBA7629911.1"
                     /translation="MAQQSPYSAAMAEQRHQEWLRFVDLLKNAYQNDLHLPLLNLMLT
                     PDEREALGTRVRIVEELLRGEMSQRELKNELGAGIATITRGSNSLKAAPVELRQWLED
                     VLLKSD"
     gene            complement(4326..4853)
                     /gene="yjjX"
                     /locus_tag="J1Y83_001761"
     CDS             complement(4326..4853)
                     /gene="yjjX"
                     /locus_tag="J1Y83_001761"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001339518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-canonical purine NTP phosphatase"
                     /protein_id="HBA7629912.1"
                     /translation="MHQVVCATTNPAKIQAILQAFHEIFGEGSCHIASVAVESGVPEQ
                     PFGSEETRAGARNRVANARRLLPEADFWVAIEAGIDGDSTFSWVVIENTSQRGEARSA
                     TLPLPAVILEKVREGEALGPVMSRYTGIDEIGRKEGAIGVFTAGKLTRTSVYHQAVIL
                     ALSPFHNAVYQPLQA"
     gene            4905..5552
                     /gene="gpmB"
                     /locus_tag="J1Y83_001762"
     CDS             4905..5552
                     /gene="gpmB"
                     /locus_tag="J1Y83_001762"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase GpmB"
                     /protein_id="HBA7629913.1"
                     /translation="MLQVYLVRHGETQWNAERRIQGQSDSPLTAKGEQQAMQVATRAK
                     ELGITHIISSDLGRTRRTAEIIAQACGCDIIFDSRLRELNMGVLETRNIDSLTEEEEN
                     WRRQLVNGTVDGRIPEGESMQELSDRVNAALESCRDLPQGSRPLLVSHGIALGCLVST
                     ILGLPAWAERRLRLRNCSISRVDYQESLWLASGWVVETAGDISHLDAPALDELQR"
     gene            complement(5549..6418)
                     /gene="robA"
                     /locus_tag="J1Y83_001763"
     CDS             complement(5549..6418)
                     /gene="robA"
                     /locus_tag="J1Y83_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MDR efflux pump AcrAB transcriptional activator
                     RobA"
                     /protein_id="HBA7629914.1"
                     /translation="MDQAGIIRDLLIWLEGHLDHPLSLDNVAAKAGYSKWHLQRMFKD
                     VTGHAIGAYIRARRLSKSAVALRLTARPILDIALQYRFDSQQTFTRAFKKQFAQTPAL
                     YRRSPEWSAFGIRPPLRLGEFTMPEHKFVTLEDTPLIGVTQSYSCSLEQISDFRHEMR
                     YQFWHDFLGNSPTIPPVLYGLNETRPSQDKDDEQEVFYTTALAQDQADGYVLTGHPVM
                     LQGGEYVMFTYEGLGTGVQEFILTVYGTCMPMLNLTRRKGQDIERYYPAEDAKAGDRP
                     INLRCELLIPIRR"
     gene            6629..7102
                     /gene="creA"
                     /locus_tag="J1Y83_001764"
     CDS             6629..7102
                     /gene="creA"
                     /locus_tag="J1Y83_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein CreA"
                     /protein_id="HBA7629915.1"
                     /translation="MKYKHLILSLSLIMLGPLAHAEEIGSVDTVFKMIGPDHKIVVEA
                     FDDPDVKNVTCYVSRAKTGGIKGGLGLAEDTSDAAISCQQVGPIELSDRIKNGKAQGE
                     VVFKKRTSLVFKSLQVVRFYDAKRNALAYLAYSDKVVEGSPKNAISAVPVMPWRQ"
     gene            7115..7804
                     /gene="creB"
                     /locus_tag="J1Y83_001765"
     CDS             7115..7804
                     /gene="creB"
                     /locus_tag="J1Y83_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator CreB"
                     /protein_id="HBA7629916.1"
                     /translation="MQRETVWLVEDEQGIADTLVYMLQQEGFDVEVFERGLPVLDKAR
                     QQVPDVMILDVGLPDISGFELCRQLLALHPALPVLFLTARSEEVDRLLGLEIGADDYV
                     AKPFSPREVCARVRTLLRRVKKFSTPSPVIRIGHFELNEPAAQISWFDTPLTLTRYEF
                     LLLKTLLKSPGRVWSRQQLMDSVWEDAQDTYDRTVDTHIKTLRAKLRAINPDLSPINT
                     HRGMGYSLRGL"
     gene            7804..9228
                     /gene="creC"
                     /locus_tag="J1Y83_001766"
     CDS             7804..9228
                     /gene="creC"
                     /locus_tag="J1Y83_001766"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     CreC"
                     /protein_id="HBA7629917.1"
                     /translation="MRIGMRLLLGYFLLVAVAAWFVLAIFVKEVKPGVRRATEGTLID
                     TATLLAELARPDLLSGDPTHGQLAQAFNQLQHRPFRANIGGINKVRNEYHVYMTDAQG
                     KVLFDSANKAVGQDYSRWNDVWLTLRGQYGARSTLQNPADPESSVMYVAAPIMDGSRL
                     IGVLSVGKPNAAMAPVIKRSERRILWASAILLGIALVIGAGMVWWINRSIARLTRYAD
                     SVTDNKPVPLPDLGSSELRKLAQALESMRVKLEGKNYIEQYVYALTHELKSPLAAIRG
                     AAEILREGPPPEVVARFTDNILTQNARMQALVETLLRQARLENRQEVVLTAVDVAALF
                     RRVSEARTVQLAEKKITLHVTPTEVNVAAEPALLEQALGNLLDNAIDFTPESGCITLS
                     AEVDQEHVTLKVLDTGSGIPDYALSRIFERFYSLPRANGQKSSGLGLAFVSEVARLFN
                     GEVTLRNVQEGGVLASLRLHRHFT"
     gene            9286..10638
                     /gene="creD"
                     /locus_tag="J1Y83_001767"
     CDS             9286..10638
                     /gene="creD"
                     /locus_tag="J1Y83_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein CreD"
                     /protein_id="HBA7629918.1"
                     /translation="MLKSPLFWKMTTLFGAVLLLLIPIMLIRQVIVERADYRSDVEDA
                     IRQSTSGPQKLVGPLIAIPVTEIYTVQEEDKTVERKRSFIHFWLPESLMVDGNQNVEE
                     RKIGIYTGQVWHSDLTLKADFDVSRLSELNAPNITLGKPFIVISVGDARGIGVVKAPE
                     VNGTALTIEPGTGLEQGGQGVHIPLPEGDWRKQNLKLNMALNLSGTGDLSVVPGGRNS
                     EMTLTSNWPHPSFLGDFLPAKREVSESGFQAHWQSSWFANNLGERFASGNDTGWENFP
                     AFSVAVTTPADQYQLTDRATKYAILLIALTFMAFFVFETLTAQRLHPMQYLLVGLSLV
                     MFYLLLLALSEHTGFTVAWIIASLIGALMNGIYLQAVLKGWRNSMLFTLALLLLDGVM
                     WGLLNSADSALLLGTSVLVVALAGMMFVTRNIDWYAFSLPKMKASKEVTTDDELRIWK
                     "
     gene            complement(10698..11414)
                     /gene="arcA"
                     /locus_tag="J1Y83_001768"
     CDS             complement(10698..11414)
                     /gene="arcA"
                     /locus_tag="J1Y83_001768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003856501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator ArcA"
                     /protein_id="HBA7629919.1"
                     /translation="MQTPHILIVEDELVTRNTLKSIFEAEGYDVFEATDGAEMHQILS
                     EYDINLVIMDINLPGKNGLLLARELREQANVALMFLTGRDNEVDKILGLEIGADDYIT
                     KPFNPRELTIRARNLLSRTMNLGTVSEERRSVESYKFNGWELDINSRSLIGPDGEQYK
                     LPRSEFRAMLHFCENPGKIQSRAELLKKMTGRELKPHDRTVDVTIRRIRKHFESTPDT
                     PEIIATIHGEGYRFCGDLED"
     gene            11510..11650
                     /locus_tag="J1Y83_001769"
     CDS             11510..11650
                     /locus_tag="J1Y83_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA7629920.1"
                     /translation="MTKVRNCVLDALSINVNNIISLVVGTFPQDPTVSKTAVILTILT
                     AI"
     gene            12050..12736
                     /locus_tag="J1Y83_001770"
     CDS             12050..12736
                     /locus_tag="J1Y83_001770"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA/rRNA methyltransferase"
                     /protein_id="HBA7629921.1"
                     /translation="MRITIILVAPARAENIGAAARAMKTMGFSELRIVDSQAHLEPAA
                     RWVAHGSGDIIDNIKVFKTLAESLHDVDFTVATTARSRAKYHYYATPVELVPLLEEKS
                     SWMSHAALVFGREDSGLTNEELALADVLTGVPMVADYPSLNLGQAVMVYCYQLATLIQ
                     QPAKSDTTADQHQLQALRERAMTLLTTLAVADDIKLVDWLQQRLGLLEQRDTAMLHRL
                     LHDIEKNITK"
     gene            12950..13015
                     /gene="thrL"
                     /locus_tag="J1Y83_001771"
     CDS             12950..13015
                     /gene="thrL"
                     /locus_tag="J1Y83_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thr operon leader peptide"
                     /protein_id="HBA7629922.1"
                     /translation="MKRISTTITTTITITTGNGAG"
     gene            13096..15558
                     /gene="thrA"
                     /locus_tag="J1Y83_001772"
     CDS             13096..15558
                     /gene="thrA"
                     /locus_tag="J1Y83_001772"
                     /EC_number="1.1.1.3"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aspartate kinase/homoserine
                     dehydrogenase I"
                     /protein_id="HBA7629923.1"
                     /translation="MRVLKFGGTSVANAERFLRVADILESNARQGQVATVLSAPAKIT
                     NHLVAMIEKTISGQDALPNISDAERIFAELLTGLAAAQPGFPLAQLKTFVDQEFAQIK
                     HVLHGISLLGQCPDSINAALICRGEKMSIAIMAGVLEARGHNVTVIDPVEKLLAVGHY
                     LESTVDIAESTRRIAASRIPADHMVLMAGFTAGNEKGELVVLGRNGSDYSAAVLAACL
                     RADCCEIWTDVDGVYTCDPRQVPDARLLKSMSYQEAMELSYFGAKVLHPRTITPIAQF
                     QIPCLIKNTGNPQAPGTLIGASRDEDELPVKGISNLNNMAMFSVSGPGMKGMVGMAAR
                     VFAAMSRARISVVLITQSSSEYSISFCVPQSDCVRAERAMQEEFYLELKEGLLEPLAV
                     TERLAIISVVGDGMRTLRGISAKFFAALARANINIVAIAQGSSERSISVVVNNDDATT
                     GVRVTHQMLFNTDQVIEVFVIGVGGVGGALLEQLKRQQSWLKNKHIDLRVCGVANSKA
                     LLTNVHGLNLENWQEELAQAKEPFNLGRLIRLVKEYHLLNPVIVDCTSSQAVADQYAD
                     FLREGFHVVTPNKKANTSSMDYYHQLRYAAEKSRRKFLYDTNVGAGLPVIENLQNLLN
                     AGDELMKFSGILSGSLSYIFGKLDEGMSFSEATTLAREMGYTEPDPRDDLSGMDVARK
                     LLILARETGRELELADIEIEPVLPAEFNAEGDVAAFMANLSQLDDLFAARVAKARDEG
                     KVLRYVGNIDEDGVCRVKIAEVDGNDPLFKVKNGENALAFYSHYYQPLPLVLRGYGAG
                     NDVTAAGVFADLLRTLSWKLGV"
     gene            15560..16492
                     /gene="thrB"
                     /locus_tag="J1Y83_001773"
     CDS             15560..16492
                     /gene="thrB"
                     /locus_tag="J1Y83_001773"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HBA7629924.1"
                     /translation="MVKVYAPASSANMSVGFDVLGAAVTPVDGALLGDVVTVEAAETF
                     SLNNLGRFADKLPSEPRENIVYQCWERFCQELGKQIPVAMTLEKNMPIGSGLGSSACS
                     VVAALMAMNEHCGKPLNDTRLLALMGELEGRISGSIHYDNVAPCFLGGMQLMIEENDI
                     ISQQVPGFDEWLWVLAYPGIKVSTAEARAILPAQYRRQDCIAHGRHLAGFIHACYSRQ
                     PELAANLMKDVIAEPYRERLLPGFRQARQAVAEIGAVASGISGSGPTLFALCDKPDTA
                     QRVADWLGKNYLQNQEGFVHICQLDTAGARVLEN"
     gene            16493..17779
                     /gene="thrC"
                     /locus_tag="J1Y83_001774"
     CDS             16493..17779
                     /gene="thrC"
                     /locus_tag="J1Y83_001774"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HBA7629925.1"
                     /translation="MKLYNLKDHNEQVSFAQAVTQGLGKNQGLFFPHDLPEFSLTEID
                     EMLKLDFVTRSAKILSAFIGDEIPQEILEERVRAAFAFPAPVANVESDVGCLELFHGP
                     TLAFKDFGGRFMAQMLTHIAGDKPVTILTATSGDTGAAVAHAFYGLPNVKVVILYPRG
                     KISPLQEKLFCTLGGNIETVAIDGDFDACQALVKQAFDDEELKVALGLNSANSINISR
                     LLAQICYYFEAVAQLPQEARNQLVVSVPSGNFGDLTAGLLAKSLGLPVKRFIAATNVN
                     DTVPRFLHDGQWSPKATQATLSNAMDVSQPNNWPRVEELFRRKIWQLKELGYAAVDDE
                     TTQQTMRELKELGYTSEPHAAVAYRALRDQLNPGEYGLFLGTAHPAKFKESVEAILGE
                     TLDLPKELAERADLPLLSHNLPADFAALRKLMMNHQ"
     gene            17993..18289
                     /locus_tag="J1Y83_001775"
     CDS             17993..18289
                     /locus_tag="J1Y83_001775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2502 domain-containing protein"
                     /protein_id="HBA7629926.1"
                     /translation="MKKMQSIVLALSLVLVAPMAAQAAEITLVPSVKLQIGDRDNRGY
                     YWDGGHWRDHGWWKQHYEWRGNRWHPYGPPPPPRHHKKAPHDHHGGHGPGKHHR"
     gene            complement(18442..19218)
                     /gene="yaaA"
                     /locus_tag="J1Y83_001776"
     CDS             complement(18442..19218)
                     /gene="yaaA"
                     /locus_tag="J1Y83_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide stress protein YaaA"
                     /protein_id="HBA7629927.1"
                     /translation="MLILISPAKTLDYQSPLTTTRYTLPELLDNSQQLIHEARKLTPP
                     QISSLMRISDKLAGINAARFHDWQPDFTPENARQAILAFKGDVYTGLQAETFSEDDFD
                     FAQQHLRMLSGLYGVLRPLDLMQPYRLEMGIRLENARGKDLYQFWGDIITNKLNEALA
                     AQGDNVVINLASDEYFKSVKPKKLNAEIIKPVFLDEKNGKFKIISFYAKKARGLMSRF
                     IIENRLTKPEQLTGFNSEGYFFDEAYSSNGELVFKRYEQR"
     gene            complement(19288..20718)
                     /locus_tag="J1Y83_001777"
     CDS             complement(19288..20718)
                     /locus_tag="J1Y83_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:alanine symporter family protein"
                     /protein_id="HBA7629928.1"
                     /translation="MPDFFSFINSVLWGSVMIYLLFGAGCWFTFRTGFVQFRYIRQFG
                     KSLKNSIHPQPGGLTSFQSLCTSLAARVGSGNLAGVALAITAGGPGAVFWMWVAAFIG
                     MATSFAECSLAQLYKERDANGQFRGGPAWYMARGLGMRWMGVLFAVFLLIAYGIIFSG
                     VQANAVARALSFSFDFPPLVTGIILAVFALLAITRGLHGVARLMQGFVPLMAIIWVLT
                     SLVICVMNIGQLPHVIWSIFESAFGWQEAAGGATGYTLSQAITNGFQRSMFSNEAGMG
                     STPNAAAAAASWPPHPAAQGIVQMIGIFIDTLVICTASAMLILLAGNGTTYMPLEGIQ
                     LIQKAMRVLMGSWGAEFVTLVVILFAFSSIVANYIYAENNLFFLRLNNPKAIWCLRIC
                     TFATVIGGTLLSLPLMWQLADIIMACMAITNLTAILLLSPVVHTIASDYLRQRKLGVR
                     PVFDPLRYPEIGRQLSPDAWDDVSQE"
     gene            20997..21950
                     /gene="tal"
                     /locus_tag="J1Y83_001778"
     CDS             20997..21950
                     /gene="tal"
                     /locus_tag="J1Y83_001778"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HBA7629929.1"
                     /translation="MTDKLTSLRQYTTVVADTGDIAAMKLYQPQDATTNPSLILNAAQ
                     IPEYRKLIDDAVAWAKQQSNDRAQQIVDATDKLAVNIGLEILKLVPGRISTEVDARLS
                     YDTEASITKAKRLIKLYNDAGISNDRILIKLASTWQGIRAAEQLEKEGINCNLTLLFS
                     FAQARACAEAGVFLISPFVGRILDWYKANTDKKEYAPAEDPGVVSVSEIYQYYKEHGY
                     ETVVMGASFRNIGEILELAGCDRLTIAPALLKELAESEGAIERKLSYTGEVKARPARI
                     TESEFLWQHNQDPMAVDKLAEGIRKFAVDQEKLEKMIGDLL"
     gene            22065..22652
                     /gene="mog"
                     /locus_tag="J1Y83_001779"
     CDS             22065..22652
                     /gene="mog"
                     /locus_tag="J1Y83_001779"
                     /EC_number="2.7.7.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin adenylyltransferase"
                     /protein_id="HBA7629930.1"
                     /translation="MNTLRIGLVSISDRASSGVYQDKGIPALEEWLTSALTTPFELET
                     RLIPDEQAIIEQTLCELVDEMSCHLVLTTGGTGPARRDVTPDATLAVADREMPGFGEQ
                     MRQISLHFVPTAILSRQVGVIRKQALILNLPGQPKSIKETLEGVKDAEGNVVVHGIFA
                     SVPYCIQLLEGPYVETAPEVVAAFRPKSARREVSE"
     gene            complement(22857..23423)
                     /gene="satP"
                     /locus_tag="J1Y83_001780"
     CDS             complement(22857..23423)
                     /gene="satP"
                     /locus_tag="J1Y83_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate uptake transporter"
                     /protein_id="HBA7629931.1"
                     /translation="MGNTKLANPAPLGLMGFGMTTILLNLHNVGYFALDGIILAMGIF
                     YGGIAQIFAGLLEYKKGNTFGLTAFTSYGSFWLTLVAILLMPKLGLTDAPNAQFLGVY
                     LGLWGIFTLFMFFGTLKGARVLQFVFFSLTVLFALLAIGNIAGNAAIIHFAGWIGLIC
                     GASAIYLAMGEVLNEQFGRTVLPIGESH"
     gene            complement(23572..24285)
                     /gene="msyB"
                     /locus_tag="J1Y83_001781"
     CDS             complement(23572..24285)
                     /gene="msyB"
                     /locus_tag="J1Y83_001781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acidic protein MsyB"
                     /protein_id="HBA7629932.1"
                     /translation="MNVNYLNDSDLDFLQHCSEEQLANFARLLTHNEKGKTRLSSVLM
                     RNELFKSMEGHPERHRRNWQLIAGELQHFGGDSIANKLRGHGKLYRAILLDVSKRLKL
                     KADKEMSTFEIEQQLLEQFLRNTWKKMDEEHKQEFLHAVDARVNELEELLPLLMKDKL
                     LAKGVSHLLSSQLTRILRTHAAMSVLGHGLLRGAGLGGPVGAALNGVKAVSGSAYRVT
                     IPAVLQIACLRRMVSATQV"
     gene            complement(24311..24715)
                     /locus_tag="J1Y83_001782"
     CDS             complement(24311..24715)
                     /locus_tag="J1Y83_001782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2541 family protein"
                     /protein_id="HBA7629933.1"
                     /translation="MKSVITISASLAISLMLCCTAQANDHKILGVIAMPRNETNDLAL
                     KLPVCRIVKRIQLSADHGDLQLSGASIYFKATRSASQTLNIPSEIKEGQTTDWININS
                     DNDNKRCVSKITFSGHTVNSSDMATLKIIGDD"
     gene            25087..27003
                     /gene="dnaK"
                     /locus_tag="J1Y83_001783"
     CDS             25087..27003
                     /gene="dnaK"
                     /locus_tag="J1Y83_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001737966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HBA7629934.1"
                     /translation="MGKIIGIDLGTTNSCVAIMDGTTPRVLENAEGDRTTPSIIAYTQ
                     DGETLVGQPAKRQAVTNPQNTLFAIKRLIGRRFQDEEVQRDVSIMPFKIIAADNGDAW
                     VEVKGQKMAPPQISAEVLKKMKKTAEDYLGEPVTEAVITVPAYFNDAQRQATKDAGRI
                     AGLEVKRIINEPTAAALAYGLDKGTGNRTIAVYDLGGGTFDISIIEIDEVDGEKTFEV
                     LATNGDTHLGGEDFDSRLINYLVEEFKKDQGIDLRNDPLAMQRLKEAAEKAKIELSSA
                     QQTDVNLPYITADATGPKHMNIKVTRAKLESLVEDLVNRSIEPLKVALQDAGLSVSDI
                     DDVILVGGQTRMPMVQKKVAEFFGKEPRKDVNPDEAVAIGAAVQGGVLTGDVKDVLLL
                     DVTPLSLGIETMGGVMTTLIAKNTTIPTKHSQVFSTAEDNQSAVTIHVLQGERKRAAD
                     NKSLGQFNLDGINPAPRGMPQIEVTFDIDADGILHVSAKDKNSGKEQKITIKASSGLN
                     EDEIQKMVRDAEANAEADRKFEELVQTRNQGDHLLHSTRKQVEEAGDKLPADDKTAIE
                     SALTALETALKGEDKAAIEAKMQELAQVSQKLMEIAQQQHAQQQTAGADASANNAKDD
                     DVVDAEFEEVKDKK"
     gene            27092..28222
                     /gene="dnaJ"
                     /locus_tag="J1Y83_001784"
     CDS             27092..28222
                     /gene="dnaJ"
                     /locus_tag="J1Y83_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HBA7629935.1"
                     /translation="MAKQDYYEILGVSKTAEEREIKKAYKRLAMKYHPDRNQGDKEAE
                     AKFKEIKEAYEVLTDSQKRAAYDQYGHAAFEQGGMGGGGFGGGADFSDIFGDVFGDIF
                     GGGRGRQRAARGADLRYNMELTLEEAVRGVTKEIRIPTLEECDVCHGSGAKPGTQPQT
                     CPTCHGSGQVQMRQGFFAVQQTCPHCQGRGTLIKDPCNKCHGHGRVERSKTLSVKIPA
                     GVDTGDRIRLAGEGEAGEHGAPAGDLYVQVQVKQHPIFEREGNNLYCEVPINFAMAAL
                     GGEIEVPTLDGRVKLKVPGETQTGKLFRMRGKGVKSVRGGAQGDLLCRVVVETPVGLN
                     EKQKQLLQELQESFGGPTGEHNSPRSKSFFDGVKKFFDDLTR"
     gene            complement(28327..28536)
                     /gene="mokC"
                     /locus_tag="J1Y83_001785"
     CDS             complement(28327..28536)
                     /gene="mokC"
                     /locus_tag="J1Y83_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin MokC"
                     /protein_id="HBA7629936.1"
                     /translation="MQNTCRVPLTDRKVKEKRAMKQHKAMIVALIVICITAVVAALVT
                     RKDLCEVHIRTGQTEVAVFTAYESE"
     gene            29094..>29661
                     /locus_tag="J1Y83_001786"
     CDS             29094..>29661
                     /locus_tag="J1Y83_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001526572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA7629937.1"
                     /translation="MSAKRRLLIACTLITAIYHFPAYSSLEYKGTFGSINAGYADWNS
                     GFVNTHRGEVWKVTADFGVNFKEAEFYSFYESNVLNHAVAGRNHTVSAMTHVRLFDSD
                     MTFFGKIYGQWDNSWGDDLDMFYGFGYLGWNGEWGFFKPYIGLHNQSGDYVSAKYGQT
                     NGWNGYVVGWTAVLPFTLFDEKFVLSNWN"
BASE COUNT         7419 a         7266 c         7866 g         7110 t
ORIGIN      
        1 cgcgccgcat ccggcatttt acgcattact tcgcaatacg cttgtacttg atacgcttcg
       61 gctccagcgc gtctgcgccc agcgtgcgtt tcttgtactc ttcgtactcg gtaaagttac
      121 cttcaaagaa ctcaacttta ccttcatcct ggtaatccag aatgtgcgtg gcgatacggt
      181 cgaggaacca acggtcgtgc gagataacca tcgcacagcc cgggaactcc agcagggcat
      241 tttccagcgc gcgcagggtt tcgatatcca ggtcgttggt cggttcgtcg agcagcagca
      301 tgttgccgcc aacctgcagc agcttcgcca gatgcagacg accgcgctca ccgccggaga
      361 gttcgccaac gcgtttaccc tgatcaaccc ctttaaagtt aaagcggcca acgtaggcgc
      421 ggctcggcat ctcggtgttg ccgatcttca tgatatccag cccgccggaa acttcttccc
      481 aaacggtttt gctgttatcc attgagtcac ggaactgatc aaccgacgcc agtttcaccg
      541 tttcacccaa agtgatggtg ccgctgtccg gctgttcctg accagagatc atacggaaca
      601 gtgtcgattt acccgcaccg ttcggaccga tgatcccgac gatcgctcct ttcgggatcg
      661 agaagctcag ggagtcaatc agcagacgat caccatagga tttacgcagg ttgctgactt
      721 ccagcacttt atcgcccaga cgcggtccag gtggaataaa cagttcgttg gtttcgttac
      781 gtttctgata ttcggtgctg ttgagttctt caaagcgcgc cagacgtgct ttaccttttg
      841 actgacggcc tttagttccc tgacgtaccc actccagctc tttctcgatc gacttacgac
      901 gtgccgcttc ttgtgaagct tcctgcgcca gacgctgatc tttctgttcc agccaggagg
      961 agtagttacc ttcccacgga ataccttcac cgcggtcaag ttcgaggatc cagcccgcaa
     1021 cgttatcgag gaagtaacgg tcgtgggtaa tcgccaccac ggtgccttcg aagtcgtgca
     1081 ggaagcgttc cagccacgcc acggattcgg catccaggtg gttggtcggt tcgtcgagca
     1141 gcagcatgtc tggtttttcc agcagcaggc ggcacaacgc cacgcgacga cgctcaccac
     1201 cggagaggtt agcgattttc gcgtcccagt ccggcagacg cagcgcatcc gccgcacgct
     1261 ccagctgaac gttcaggttg tgaccgtcgt gagcctgaat gatctcttcc agacggcctt
     1321 gctcagcggc cagcttgtca aaatcggcat ccggatcggc atacagcgca tacacttcat
     1381 ccaggcgttt cagggcgtta accacttcag aaaccgcttc ttcaatggac tcacgaacgg
     1441 tgtgttccgg gttcagctgc ggttcctgcg gcagataacc aatcttgatg tctggctgcg
     1501 gacgtgcttc accttcgatg tctttatcaa tgcccgccat aatgcgcagc agggtggact
     1561 tacccgcgcc gttcagaccc aggacaccaa tttttgcccc agggaagaaa ctcagagaga
     1621 tgtttttcaa aatatgacgt ttcggcggaa caactttgcc gacacgatgc atggtataaa
     1681 cgaattgagc cacgttggac ttcgcctcta tgtttatcgt gatgatgagt tttcaaaggc
     1741 gaagtgtagc ctgtttccct gtctaatccc agccagtcga tcaccccgta aaagtaaaaa
     1801 agtgtccgta acgtggcgta aacggcaatg actggttagc ataaatctat tacgcggcat
     1861 gacgctgcat tgatgtattg acacttagag gatgcgcttg tggaaaaagc caaacacgtt
     1921 acctggcggc tgttggctgt cggtgtctgt ctgctgacgg tcagcagcgt ggcgcgagcc
     1981 gactcactgg atgagcagcg tagtcgttac gcgcaaatca agcaggcctg ggataatcga
     2041 caaatggatg tggtcgaaca aatgatgcct ggactgaaag attacccact ttatccctac
     2101 ctggaatacc gccagatcac tgatgacctg atgaatcaac cggcggtgac ggtcactaac
     2161 tttgttcgcg ctaaccccac gcttcctccc gctcgcacgc tgcaatctcg tttcgtcaat
     2221 gaactggcgc ggcgtgaaga ctggcgtggc ttgttagcct ttagcccgga aaaacccgga
     2281 actaccgaag cgcaatgtaa ttactactat gcgaaatgga acaccgggca gagtgaagaa
     2341 gcctggcaag gggcgaaaga gctgtggcta accggcaaga gccagcctaa cgcctgtgac
     2401 aagttattta gcgtctggcg tgcgtcaggt aaacaagatc cgctggcgta tttagagcgt
     2461 atccgtctgg cgatgaaagc gggtaacacc ggcctggtaa cagtactggc agggcagatg
     2521 cctgccgatt accagactat cgcttcggca atcatttcac tggcgaacaa ccctaatacg
     2581 gtactgacct tcgcgcgtac aaccggcgcg accgatttta cccgtcaaat ggcggcggtg
     2641 gcgtttgcca gtgtggcgcg gcaggatgct gaaaatgcac ggctgatgat cccgtcgctt
     2701 gctcaggcac agcagcttaa tgaagatcag attcaggagc tgcgcgatat tgtcgcctgg
     2761 cgtttgatgg gcaacgatgt caccgacgag caggcgaaat ggcgcgatga cgcgattatg
     2821 cgctcgcaat ctacttcgct tattgaacgc cgtgtgcgaa tggcgcttgg caccggcgat
     2881 cgtcgcggcc tgaatacctg gctggcgcgt ctgcctatgg aagcgaaaga gaaagatgaa
     2941 tggcgttact ggcaggcgga tttactgctg gaacgcggac gtgaagctga agcaaaagag
     3001 attttgcatc aactcatgca acagcgtggt ttctacccga tggttgctgc acaacgcatc
     3061 ggcgaagagt atgagctgaa gattgataaa gcgccgcaga atgttgacag cgccctgact
     3121 caggggccgg agatggcgcg cgtgcgcgag ttgatgtact ggaatctcga taacaccgcg
     3181 cgtagcgagt gggccaatct ggtgaagagc aagtcaaaaa cagagcaggc tcaactggct
     3241 cggtatgctt tcaataacca atggtgggat cttagcgttc aggcaacgat cgccgggaag
     3301 ctttgggatc atctggaaga gcgattcccg ctggcttaca acgatctttt caaacgctac
     3361 accagtggta aggagatccc gcaaagctat gcgatggcga ttgcccgtca ggagagtgcc
     3421 tggaatccga aagtgaaatc accggtaggg gccagcggcc tgatgcagat tatgcctggt
     3481 accgcgaccc atacggtgaa gatgttctct attcccggtt atagcagccc tgggcaattg
     3541 ctggatccgg aaacgaacat caacattggc accagttacc tgcaatatgt ttatcagcag
     3601 tttggcaata accgtatttt ctcctcagca gcttataacg ccggaccagg gcgggtgcga
     3661 acctggcttg gcaacagcgc aggacgtatc gacgcagtgg catttgtcga gagtattcca
     3721 ttctccgaga cgcgcggtta tgtgaaaaac gtgctggctt atgacgctta ctaccgctat
     3781 ttcatggggg ataaaccgac gttgatgagc gccacggaat ggggacgtcg ttactgatcc
     3841 acacgtttat gatatgctat cgtactcttt agcgagtaca accgggggag gcattttgct
     3901 tcccccgcta acaatggcga catactatgg cccaacaatc accctattca gcagcgatgg
     3961 cagaacagcg tcaccaggag tggttacgtt ttgtcgacct gcttaagaat gcctaccaaa
     4021 acgatctcca tttaccgttg ttaaacctga tgctgacgcc agatgagcgc gaagcgttgg
     4081 ggactcgcgt gcgtattgtc gaagagctgt tgcgcggcga aatgagccag cgtgagttaa
     4141 aaaatgaact cggcgcgggc atcgcgacga ttacgcgtgg atctaatagt ctgaaagccg
     4201 cgcccgtcga gctgcgccag tggctggaag acgtgttgct gaaaagcgat tgattttgta
     4261 ggcctgataa gacatggcgc atcaggcatc gtgcaccgaa tgccggatgc ggcgtgaacg
     4321 cctaatcagg cctgcaatgg ctgatacacg gcattatgaa acggactcag cgccaggatc
     4381 accgcctggt gatagacgct ggtgcgagtg agtttcccgg cggtaaacac gccgatcgcc
     4441 ccttccttac gaccaatctc atcaataccg gtataacgcg acatcacggg accaagcgcc
     4501 tcaccttcac gcactttttc cagaatcacc gcaggcaacg gcaaagtagc cgaacgcgcc
     4561 tcgccgcgct ggctggtgtt ttcaatcacc acccaactga aagtgctgtc accatcgatg
     4621 ccagcttcaa tcgccaccca aaaatcagcc tcaggaagta aacggcgggc attggctacc
     4681 ctatttcgtg cgccagcgcg cgtttcctca ctgccaaagg gctgttccgg tacaccgctc
     4741 tcgacggcaa cggatgcaat atggcaggat ccttcgccga agatctcgtg aaatgcctgc
     4801 agaatggcct gaattttagc gggattggtg gtcgcacaga caacttggtg cataatcagc
     4861 attactcaga aaattaacgt tacagcagta tacggaaaaa aagcatgtta caggtatacc
     4921 tagtccgcca cggtgaaacg cagtggaacg ccgagcgacg tattcagggc cagtctgaca
     4981 gcccgctgac cgccaaaggt gagcaacagg cgatgcaggt ggcaacccgt gccaaagagc
     5041 ttggcattac gcatatcatc agtagcgatt taggacgcac ccggcgcacg gcggaaatca
     5101 tcgcccaggc ctgcggctgt gacatcatct ttgattctcg cctgcgtgaa ttaaatatgg
     5161 gtgtactgga aacaagaaat atcgattcac tcaccgaaga agaagagaac tggcgtcggc
     5221 agctggtcaa tggcaccgtt gacgggcgta ttcctgaagg cgagtcaatg caggagctca
     5281 gcgatcgcgt caatgcggca ctggaatctt gccgggactt accgcaggga agccgaccgc
     5341 tgctggtgag ccatggtatt gcactgggat gcctggtgag tacgattctc ggattaccag
     5401 catgggcaga gcgccgctta cgtctgcgta actgttctat ttcgcgcgtg gattatcagg
     5461 aaagcctgtg gctagcgtct ggctgggtgg ttgaaaccgc aggggacatc tcgcatctgg
     5521 acgcccctgc attagatgag ttgcagcgtt aacgacggat cggaatcagc agttcacagc
     5581 gcagattaat tgggcgatct cccgctttgg catcttctgc cgggtagtat cgctcaatat
     5641 cctgaccttt acggcgcgtc aggttaagca ttggcatgca cgttccgtat accgtcagga
     5701 taaactcctg cacgccggtt cccagacctt cataggtaaa catcacatat tcgccgcctt
     5761 gcagcatcac cggatgcccg gtcaatacat agccatctgc ctgatcctga gctaacgcgg
     5821 tggtgtagaa cacctcttgt tcgtcgtctt tatcctggct cggacgtgtt tcgttcaaac
     5881 cgtaaagcac tggtggaatg gttggcgagt tgccgagaaa atcgtgccag aactggtagc
     5941 gcatttcatg gcggaaatca gagatttgct ccagcgaaca ggagtagctc tgggtaacac
     6001 caatcagcgg cgtatcttcc agggtgacaa atttgtgctc tggcatagtg aattcaccca
     6061 gacgcagcgg cgggcgaata ccaaaggcgc tccattcagg agaacggcgg taaagtgcag
     6121 gagtctgggc aaactgcttc ttgaaagcgc gggtaaatgt ctgttgagag tcgaagcggt
     6181 attgcagcgc gatgtccaga atcggacgcg cagtcaggcg tagtgcgacc gccgatttcg
     6241 acaaacgacg agcacgaata tacgcgccaa tagcatggcc agtgacatct ttaaacattc
     6301 tctgtaagtg ccacttggaa taacctgctt tcgccgctac attgtcgagc gacaggggat
     6361 gatccagatg accttccagc cagattaaaa ggtcgcgaat aatgccggcc tgatccataa
     6421 aatatcctca tcctttcaac aacgagcacc tgacatcagg taattggata atagcatttt
     6481 ttgctgtttt agcattcagt gtttttttct tagtcgagta tgttttaggt cttgacggaa
     6541 ataaaagtat tgagattttg ttcttaatca atatgttatt taccgtgacg aactaattgc
     6601 tcgtgtaata gataaaaatg gtaacgatat gaaatacaag catttaatcc tgtctttaag
     6661 cctgataatg ctggggccat tggctcatgc agaagagatt ggttcggtcg acaccgtatt
     6721 taaaatgatc ggcccggatc acaaaattgt tgttgaagcc tttgacgatc ccgatgtgaa
     6781 aaatgtcacc tgttatgtga gccgggcgaa aaccggtggt attaaagggg gattgggcct
     6841 ggcagaagat acctccgatg cggccatttc ttgtcaacaa gtcgggccga ttgaactatc
     6901 ggatcgtatt aaaaacggca aagctcaggg cgaggtagta ttcaaaaaac gcacgtccct
     6961 ggtctttaag tcgttacagg tcgtgcgctt ttatgacgct aaacgcaacg cgctcgctta
     7021 tctggcttac tccgacaaag ttgtagaagg ttcgccgaaa aacgcgatta gcgcggttcc
     7081 tgtcatgccg tggcggcaat aacagaggcg atttatgcaa cgggaaacgg tctggttagt
     7141 cgaagacgag caagggatag ccgatacgct ggtctacatg ttgcaacagg aaggttttga
     7201 cgtcgaggtc tttgagcgag gcttgccagt gctggataaa gctcgccagc aggttcccga
     7261 tgtcatgatt ctcgatgttg gtttgcctga tatcagcggc tttgaattgt gccgccagtt
     7321 actggcgctc catccggcgt tacctgtact gttcctgacg gcccgaagtg aagaggtcga
     7381 tcgcctgctt gggctggaaa ttggcgctga cgactacgtg gctaaaccgt tttcacctcg
     7441 cgaagtgtgc gccagagtgc gcaccttact gcgtcgggtg aagaagttct cgacgccgtc
     7501 tcccgttatc cgtattggtc attttgaatt gaatgaacct gcggcgcaga tcagctggtt
     7561 tgacacgcca ttaacgctga cgcggtatga gtttttattg ctgaagacgt tactcaagtc
     7621 gccgggccgc gtctggtccc gccagcaact aatggatagc gtgtgggaag atgcgcagga
     7681 cacctacgat cgcaccgtcg atacccacat taaaacgctg cgtgccaagc tgcgcgccat
     7741 caaccccgat ctttcaccga ttaatactca tcgcggcatg ggatatagcc tgaggggcct
     7801 gtaatgcgca tcggcatgcg gttgttgctg ggctattttt tactggtggc ggtggcggcc
     7861 tggttcgtac tggctatttt tgtcaaagaa gttaaaccgg gcgtgcgaag agcaaccgag
     7921 gggacgttaa tcgacaccgc aacgttgctg gcggagctgg cgcgtccaga tttgctctct
     7981 ggggacccaa cgcatgggca actggcgcag gcgtttaatc agctacaaca tcgcccgttt
     8041 cgcgccaata tcggtggcat taacaaagtg cgcaatgaat atcatgtcta tatgaccgat
     8101 gcgcagggca aagtattgtt cgattcggca aataaagccg ttggacagga ttattcgcgc
     8161 tggaatgacg tctggctaac gttgcgtggt cagtatggtg cgcgcagcac gttgcaaaat
     8221 cctgccgatc ccgaaagttc tgtgatgtat gttgccgcac cgattatgga cggctcgcgg
     8281 cttattggcg ttttgagcgt aggcaaaccg aacgcggcga tggctccggt cattaagcgt
     8341 agcgagcggc gaattttatg ggccagcgcc attttgttgg ggattgcact ggtgattggc
     8401 gcaggcatgg tttggtggat caaccgctct attgcccggc tcactcgcta tgctgattcc
     8461 gtcactgaca ataagcccgt tcctctcccc gatctcggta gtagcgagtt gcgtaaactc
     8521 gcgcaggcgc tggaaagtat gcgcgtgaag ctggaaggga aaaactatat tgagcagtat
     8581 gtttacgcat taactcatga gctaaaaagc ccactggcgg cgattcgtgg cgcggcggaa
     8641 attttacgcg aaggtccgcc gccggaagtg gtggctcgtt ttactgacaa cattctgacg
     8701 caaaatgcgc gtatgcaggc attggtagaa acgttactac gccaggcaag actggagaat
     8761 cgtcaggaag tcgttctgac tgctgttgat gtggcggcat tattccgccg cgtcagcgaa
     8821 gcgcgcaccg tgcagttggc agaaaaaaaa atcactctgc atgttacgcc caccgaggtt
     8881 aacgttgctg ctgaaccggc gttactggag caggcgctgg gaaatttact ggataacgcc
     8941 atcgatttta cccccgagag cggttgcatc acgctaagcg ccgaagtgga tcaggaacac
     9001 gtcacgctta aggtgctgga taccggtagt ggtattcctg actacgcgct ttcacgtatt
     9061 tttgaacgct tttactcttt gcctcgtgca aatgggcaaa aaagcagcgg tctggggttg
     9121 gcgttcgtca gtgaggtcgc ccgtttgttt aacggcgaag tcacgctgcg caacgtgcag
     9181 gaaggtggcg tgctggcctc gcttcgactt caccgtcact tcacatagct tcaaattctt
     9241 tccacatagc cttcgtatcc tgctgccatt gcaaaggaga agactatgtt gaaatccccc
     9301 ctgttctgga aaatgaccac cctgtttggt gcagtattgc tgttgttgat tccgataatg
     9361 ctgattcggc aggtgattgt cgaacgtgcc gattaccgta gcgatgtgga agatgcgatt
     9421 cgccaaagta ccagcgggcc gcaaaaactc gttgggccgc ttatcgctat tcctgtgacc
     9481 gagatttata cggtgcagga agaggataaa accgtggagc ggaaacgaag ttttatccat
     9541 ttttggttac ctgagtcatt gatggttgat ggcaatcaga acgtggaaga acgcaagata
     9601 gggatttata ccggtcaggt ctggcacagt gatttaacgc taaaagccga tttcgatgtt
     9661 tcgcgtctta gcgaactcaa cgcgccaaat atcaccttag gcaagccatt tattgtgatt
     9721 agcgtcgggg atgcgcgtgg tattggtgtg gtgaaagcgc ctgaagttaa cggaacggcg
     9781 ctgaccattg aacccggcac cgggttagag caaggcgggc agggcgtgca tatcccttta
     9841 cccgaagggg actggcggaa gcagaacctg aagctgaata tggcactgaa tttaagcggt
     9901 accggcgatc tttctgtggt gcctggcggg cgtaatagcg aaatgacctt aaccagcaac
     9961 tggccgcatc ccagtttttt aggtgatttt ctaccagcca aacgggaagt tagcgagtca
    10021 ggttttcagg cgcactggca aagcagctgg tttgctaata atctcggtga gcgttttgct
    10081 tcaggcaatg ataccggctg ggaaaacttc ccggcgttta gcgtcgcagt aacgacgcca
    10141 gccgatcaat accaattaac tgaccgggcg actaagtacg ccattctgct gattgcactg
    10201 acttttatgg cgttctttgt ttttgaaacg ctcaccgcgc aacgtttaca cccaatgcaa
    10261 tatttgctgg tggggctttc attggtgatg ttttatttgc tcttgctggc gctttctgaa
    10321 cataccggtt ttaccgtggc atggataatc gccagtctga ttggggcgtt aatgaacggt
    10381 atttatttgc aggcggtatt gaaaggctgg cgtaatagca tgttgtttac cctcgcgctg
    10441 ttgttgctgg atggtgtgat gtggggactg ctcaactctg ccgatagcgc gctgttgttg
    10501 ggaaccagtg tactggtggt ggcgctggcc ggcatgatgt ttgtgacccg taatatcgac
    10561 tggtatgcgt tttcactgcc gaaaatgaaa gccagtaaag aagttacaac ggacgatgaa
    10621 ttacgtatct ggaaataagg tagaaaaata aaaacggcgc taaaaagcgc cgtttttttt
    10681 gacggtggta aagccgatta atcttccaga tcaccgcaga agcgataacc ttcaccgtga
    10741 atggtggcga tgatttccgg cgtatccggc gtagattcga aatgtttacg aatacggcgg
    10801 atcgtcacgt ctacagtacg gtcgtgcggt ttcagctcac ggccagtcat tttcttcagc
    10861 agttcagcac gggactgaat tttgcctggg ttttcacaga agtgaagcat ggcgcggaac
    10921 tcgctgcgcg gcagcttgta ctgctcgcca tcagggccga tcaacgaacg gctgttgatg
    10981 tccagttccc aaccattgaa cttgtagctt tcaacgctac gacgttcttc gctgacagta
    11041 cccagattca tggtacggga cagcaggttg cgtgcacgaa tagtcagttc acgcgggttg
    11101 aacggtttgg tgatgtagtc atctgcaccg atttcgaggc cgagaatttt atcgacttcg
    11161 ttgtcacggc cagtcaggaa catcaacgca acattcgcct gctcgcgcag ttcacgcgct
    11221 aacagaagac cgttcttacc cggcagattg atatccatga tcaccaggtt gatgtcatat
    11281 tcagagagga tctgatgcat ttccgcgcca tctgtcgctt cgaaaacatc atagccttcc
    11341 gcttcgaaaa tacttttcaa cgtgttgcgt gttaccaact cgtcttcaac gataagaatg
    11401 tgcggggtct gcatgtttgc tacctaaatt gccaactaaa tcgaaacagg aagtacaaaa
    11461 gtccctgacc tgcctgatgc atgctgcaaa ttaacatgat cggcgtaaca tgactaaagt
    11521 acgtaattgc gttcttgatg cactttccat caacgtcaac aacatcatta gcttggtcgt
    11581 gggtactttc cctcaggacc cgacagtgtc aaaaacggct gtcatcctaa ccattttaac
    11641 agcaatataa caggctaaga ggggcaggac acccaataaa actacgcttc gttgacatat
    11701 atcaagttca attgtagcac gttaacagtt tgatgaaatg atcgtatcta aatgctagct
    11761 ttcgtcacat tattttaata atccaactag ttgcatcata caactaataa acctggtgaa
    11821 tccaattatc gagatttatt ttttataaaa ttatcctaag taaacagaag gatatgtagc
    11881 attttttaac aattcaatcg ttagtacagt caggaaatag tttagccttt tttaagctaa
    11941 gcaaagggct tttcctgcga cttacgttaa gaatttgtaa attcgcaacg cgtaataagt
    12001 tgacaatgat caccctgttc gcggttattt ggtcaagaag agtggcaata tgcgtataac
    12061 gattattctg gttgcacccg ccagagcaga aaatattggg gcagcggcgc gggcaatgaa
    12121 aacgatgggt tttagcgaac tgcggattgt tgatagtcag gcacacctgg agccagccgc
    12181 ccgttgggtc gcacatggat ctggtgatat tattgataat attaaagttt tcaagacact
    12241 agctgaatcg ttacacgatg tcgatttcac tgttgccacc actgcgcgca gtcgggcgaa
    12301 atatcattac tacgctacgc cagtagaact ggtgccgctg ttagaggaaa aatcttcatg
    12361 gatgagccat gccgctctgg tgtttggtcg cgaagattcc gggctgacta acgaagagtt
    12421 agcgttggct gacgttctta ctggtgtgcc gatggtggcg gattatcctt cgctcaatct
    12481 ggggcaggca gtgatggtct attgctatca attagcaaca ttaatacaac aaccggcgaa
    12541 aagtgacaca acggcagacc aacatcaact gcaagcttta cgcgaacgag ccatgacatt
    12601 gctgacgact ctggcagtgg cagatgacat aaaactggtc gactggttac aacaacgcct
    12661 ggggctttta gagcaacgag acacggcaat gttgcaccgt ttgctgcatg atattgaaaa
    12721 aaatatcacc aaataaaaaa cgccttagta agtgattttc agcttttcat tctgactgca
    12781 acgggcaata tgtctctgtg tggattaaaa aaagagtgtc tgatagcagc ttctgaacgg
    12841 gttacctgcc gtgagtaaat taaaatttta ttgacttagg tcactaaata ctttaaccaa
    12901 tataggcata gcgcacagac agataaaaat tacagagtac acaacatcca tgaaacgcat
    12961 tagcaccacc attaccacca ccatcaccat taccacaggt aacggtgcgg gctgacgcgt
    13021 acaggaaaca cagaaaaaag cccgcacctg acagtgcggg cttttttttc gaccaaaggt
    13081 aacgaggtaa caaccatgcg agtgttgaag ttcggcggta catcagtggc aaatgcagaa
    13141 cgttttctgc gggttgccga tattctggaa agcaatgcca ggcaggggca ggtggcgacc
    13201 gtcctctctg cccccgccaa aattaccaac catctggtag cgatgattga aaaaaccatt
    13261 agcggccagg atgctttacc caatatcagc gatgccgaac gtatttttgc cgaacttctg
    13321 acgggactcg ccgccgccca gccgggattt ccgctggcac aattgaaaac tttcgtcgac
    13381 caggaatttg cccaaataaa acatgtcctg catggcatca gtttgttggg gcagtgcccg
    13441 gatagcatca acgctgcgct gatttgccgt ggcgagaaaa tgtcgatcgc cattatggcc
    13501 ggcgtgttag aagcgcgtgg tcacaacgtt accgttatcg atccggtcga aaaactgctt
    13561 gcagtggggc attacctcga atctaccgtt gatattgctg aatccacccg ccgtattgcg
    13621 gcaagccgca ttccggctga ccacatggtg ctgatggctg gtttcactgc cggtaatgaa
    13681 aaaggcgagc tggtggttct gggacgcaac ggttccgact actccgctgc ggtgctggcg
    13741 gcctgtttac gcgccgattg ttgcgagatc tggacggatg ttgacggtgt ttatacctgc
    13801 gacccgcgtc aggtgcccga tgcgaggttg ttgaagtcga tgtcctacca ggaagcgatg
    13861 gagctttcct acttcggcgc taaagttctt cacccccgca ccattacccc catcgcccag
    13921 tttcagatcc cttgcctgat taaaaatacc ggaaatcctc aagcaccagg tacgctcatt
    13981 ggtgccagcc gtgatgaaga cgaattaccg gtcaagggca tttccaatct gaataacatg
    14041 gcaatgttca gcgtttccgg cccggggatg aaagggatgg ttggcatggc ggcgcgcgtc
    14101 tttgcagcga tgtcacgcgc ccgtatttcc gtggtgctga ttacgcaatc atcttccgaa
    14161 tacagtatca gtttctgcgt tccgcaaagc gactgtgtgc gagctgaacg ggcaatgcag
    14221 gaagagttct acctggaact gaaagaaggc ttactggagc cgttggcggt gacggaacgg
    14281 ctggccatta tctcggtggt aggtgatggt atgcgcacct tacgtgggat ctcggcgaaa
    14341 ttctttgccg cgctggcccg cgccaatatc aacattgtcg ccattgctca gggatcttct
    14401 gaacgctcaa tctctgtcgt ggtcaataac gatgatgcga ccactggcgt gcgcgttact
    14461 catcagatgc tgttcaatac cgatcaggtt atcgaagtgt ttgtgattgg cgtcggtggc
    14521 gttggcggtg cgctgctgga gcaactgaag cgtcagcaaa gctggttgaa gaataaacat
    14581 atcgacttac gtgtctgcgg tgttgctaac tcgaaggcac tgctcaccaa tgtacatggc
    14641 cttaatctgg aaaactggca ggaagaactg gcgcaagcca aagagccgtt taatctcggg
    14701 cgcttaattc gcctcgtgaa agaatatcat ctgctgaacc cggtcattgt tgactgtact
    14761 tccagccagg cagtggcgga tcaatatgcc gacttcctgc gcgaaggttt ccacgttgtt
    14821 acgccgaaca aaaaggccaa cacctcgtcg atggattact accatcagtt gcgttatgcg
    14881 gcggaaaaat cgcggcgtaa attcctctat gacaccaacg ttggggctgg attaccggtt
    14941 attgagaacc tgcaaaatct gctcaatgct ggtgatgaat tgatgaagtt ctccggcatt
    15001 ctttcaggtt cgctttctta tatcttcggc aagttagacg aaggcatgag tttctccgag
    15061 gcgaccacac tggcgcggga aatgggttat accgaaccgg acccgcgaga tgatctttct
    15121 ggtatggatg tggcgcgtaa gctattgatt ctcgctcgtg aaacgggacg tgaactggag
    15181 ctggcggata ttgaaattga acctgtgctg cccgcagagt ttaacgccga gggtgatgtc
    15241 gccgctttta tggcgaatct gtcacagctc gacgatctct ttgccgcgcg tgtggcgaag
    15301 gcccgtgatg aaggaaaagt tttgcgctat gttggcaata ttgatgaaga tggcgtctgc
    15361 cgcgtgaaga ttgccgaagt ggatggtaat gatccgctgt tcaaagtgaa aaatggcgaa
    15421 aacgccctgg ccttctatag ccactattat cagccgctgc cgttggtact gcgcggatat
    15481 ggtgcgggca atgacgttac agctgccggt gtctttgctg atctgctacg taccctctca
    15541 tggaagttag gagtctgaca tggttaaagt ttatgccccg gcttccagtg ccaatatgag
    15601 cgtcgggttt gatgtgctcg gggcggcggt gacacctgtt gatggtgcat tgctcggaga
    15661 tgtagtcacg gttgaggcgg cagagacatt cagtctcaac aacctcggac gctttgccga
    15721 taagctgccg tcagagccac gggaaaatat cgtttatcag tgctgggagc gtttttgcca
    15781 ggagcttggc aagcaaattc cagtggcgat gactctggaa aagaatatgc cgatcggttc
    15841 gggcttaggc tccagcgcct gttcagtggt cgcggcgctg atggcgatga atgaacactg
    15901 cggcaagccg cttaatgaca ctcgtttgct ggctttgatg ggtgagctgg aagggcgtat
    15961 ctccggcagc attcattacg acaacgtggc accgtgtttt cttggtggta tgcagttgat
    16021 gattgaagaa aacgacatca tcagtcagca agtgccaggg tttgatgagt ggctgtgggt
    16081 gctggcgtat ccggggatta aagtctcgac ggcagaagcc cgggctattt taccggcgca
    16141 gtatcgccgc caggattgca ttgcgcacgg gcgacatttg gcaggcttca ttcacgcctg
    16201 ctattcccgt cagcctgagc ttgccgcgaa tctgatgaaa gatgttatcg ctgaacccta
    16261 ccgtgaacgg ttactgcctg gtttccggca ggcgcggcag gcggtcgcgg aaatcggcgc
    16321 ggtagcgagc ggtatctccg gctccggccc gactttgttc gctctgtgtg acaagccgga
    16381 taccgcccag cgcgttgccg actggttggg taagaactac ctgcaaaatc aggaaggttt
    16441 tgttcatatt tgccagctgg atacggcggg cgcacgagta ctggaaaact aaatgaaact
    16501 ctacaatctg aaagatcaca atgagcaggt cagctttgcg caagccgtaa cccaggggtt
    16561 aggcaaaaat caggggctgt tttttccgca cgacctgccg gaattcagcc tgactgaaat
    16621 tgatgagatg ctgaagctgg attttgtcac ccgcagtgcg aagatcctct cggcgtttat
    16681 tggtgatgaa atcccgcagg aaatcctgga agagcgcgta cgtgcggcgt ttgccttccc
    16741 ggctccggtc gccaatgttg aaagcgatgt cggttgtctg gaattgttcc acgggccaac
    16801 gctggcattt aaagatttcg gcggtcgctt tatggcacaa atgctgaccc atattgcggg
    16861 cgataagcca gtgaccattc tgaccgcgac atccggcgat actggagcgg cagtggctca
    16921 tgctttctac ggtttaccga atgtgaaagt ggttatcctc tatccacgag gcaaaatcag
    16981 tccactgcaa gaaaaactgt tctgtacatt gggcggcaat atcgaaactg ttgccatcga
    17041 cggcgatttc gatgcctgtc aggcgctggt gaagcaggcg tttgatgatg aagaactgaa
    17101 agtggcgctg gggctaaact ctgctaactc cattaacatc agccgtttgc tggcgcagat
    17161 ttgctactac tttgaagctg ttgcgcagct gccgcaggaa gcgcgcaacc agctggttgt
    17221 ctcggtgcca agcggaaact tcggcgattt gacggcgggt ctgctggcga agtcactcgg
    17281 tctgccggtg aaacgtttta ttgctgcgac caacgtgaac gataccgtgc cacgtttcct
    17341 gcacgacggt cagtggtcac ccaaagcgac tcaggcgacg ttatccaatg cgatggatgt
    17401 tagccagcca aacaactggc cgcgtgtgga agagttgttc cgccgcaaaa tctggcaact
    17461 gaaagagctg gggtatgcag ccgtggatga tgaaaccacg caacagacaa tgcgtgagtt
    17521 aaaagaactg ggctatacct cggagccgca cgctgccgta gcttatcgtg cgctgcgtga
    17581 ccagttgaat ccaggcgaat atggcttgtt cctcggcacc gcgcatccgg cgaaatttaa
    17641 agagagcgtg gaagcgattc tcggtgaaac gttggatctg ccaaaagagc tggcagaacg
    17701 tgctgattta cccttgcttt cgcataacct gcccgccgat tttgctgcgt tgcgtaaatt
    17761 gatgatgaat catcagtaac atctattcat tatctcaatc aggccgggtt tgcttttatg
    17821 cagcccggct tttttatgaa gaaaatatgg agaaaaacga cagggaaaaa ggagaaattc
    17881 tcaataaatg cggtaactta gagattagga ttgcggagaa taacaactgc cgttctcatc
    17941 gcgtaatctc cggatatcga cccataacgg gcaatgataa aaggagtaac ctgtgaaaaa
    18001 gatgcaatct atcgtactcg cactttccct ggttctggtc gctcccatgg cagcgcaggc
    18061 tgcggaaatt acgctagtcc cgtcagtaaa attacagata ggcgatcgtg ataatcgtgg
    18121 ctattactgg gatggcggtc actggcgaga ccacggctgg tggaaacaac attatgaatg
    18181 gcgaggcaat cgctggcacc catatggacc gccgccaccg ccgcgccacc ataagaaagc
    18241 tcctcatgat catcacggcg gtcatggtcc aggcaaacat caccgctaaa tgacaaatgc
    18301 cgggtaacaa tccggcattc agcgcctgat gcgacgctgg cgcgtcttat caggcctacg
    18361 ttaattctgc aatatattga atctgcatgc ttttgtaggc cggataaggc gttcacgccg
    18421 catccggcat ttactgcaaa cttaacgttg ttcgtagcgt ttaaacacca gttcgccatt
    18481 gctggagtaa gcttcatcaa agaagtaacc ttcgctatta aaaccagtca gttgctctgg
    18541 tttggtcagc cgattttcaa taatgaaacg actcatcaga ccgcgtgctt tcttagcgta
    18601 gaagctgatt atcttaaatt tgccgttctt ctcatcgagg aacactggct tgataatctc
    18661 ggcattcaat ttcttcggct tcaccgattt aaaatactca tctgacgcca gatttatcac
    18721 tacattatcg ccttgtgctg cgagcgcctc gttcagcttg ttggtgatga tatctcccca
    18781 gaattgatac agatctttcc ctcgggcatt ctcaagacgt atccccattt ccagacgata
    18841 aggctgcatt aaatcgagcg ggcgcagtac gccatacaag ccggaaagca ttcgcaaatg
    18901 ctgttgggca aaatcgaaat cgtcttcgct gaaggtttcg gcctgcaagc cagtgtagac
    18961 atcaccttta aacgccagaa ttgcctggcg ggcattctcc ggtgtgaaat ctggctgcca
    19021 gtcatgaaag cgagcggcgt tgatacccgc cagtttgtcg ctgatgcgca tcagggagct
    19081 aatctgcggg ggcgtcagtt tccgcgcttc atggatcagc tgctgggagt tgtctaacag
    19141 ctccggcagc gtatagcgcg tggtggtcaa cgggctttgg taatcaagcg ttttcgcagg
    19201 tgaaataaga atcagcatat ccagtccttg caggaaattt atgccgactt tagcaaaaaa
    19261 agagaatgag ttgatcgata gttgtgatta ctcctgcgaa acatcatccc acgcgtctgg
    19321 agaaagctgg cgaccgatct ccggataacg caacggatca aacaccgggc gcacgccgag
    19381 tttacgctgg cgtagataat cactggcaat ggtatgaacc acaggcgaga gcagtaatat
    19441 ggcggtcaaa ttggtaatag ccatgcaggc cataatgata tctgccagtt gccacatcag
    19501 cggaagactt agcaaggtgc cgccgatgac cgttgcgaag gtgcagatcc gcagacacca
    19561 gatcgcttta gggttgttca ggcgtaaaaa gaagagattg ttttcggcat aaatgtagtt
    19621 ggcaacgatg gagctgaagg caaacagaat aaccacgagg gtaacaaact ccgcacccca
    19681 ggaacccatt agcacccgca tcgccttctg gataagctga ataccttcca gcggcatgta
    19741 ggttgtgccg ttacccgcca gtaatatcag catggcgctt gccgtacaga tgaccagggt
    19801 gtcgataaaa atgccaatca tctggacaat tccttgcgct gccggatgcg gaggccagga
    19861 cgcagctgcc gctgccgcgt ttggcgtcga ccccattccc gcctcattgg aaaacatact
    19921 gcgctgaaaa ccgttagtaa tcgcctggct taaggtatat cccgtcgcgc cgcctgcagc
    19981 ttcctgccag ccaaaagcac tctcaaaaat agaccaaatg acgtggggaa gttgcccgat
    20041 attcattacg caaatcacca ggctggtcag tacccagatt atcgccatca acgggacaaa
    20101 cccctgcatg agccgggcga cgccatgaag accgcgagtg atcgccagca gagcaaagac
    20161 agcgagaata atacctgtta ccagcggggg aaaatcaaaa gaaaaactca gggctcgcgc
    20221 aacggcgttc gcctgaactc cgctgaaaat tatgccgtag gcgatgagca aaaagacggc
    20281 gaacagaacg cccatccagc gcatccccag cccgcgcgcc atataccatg ccggtccgcc
    20341 acgaaactgc ccattggcat cacgttcttt ataaagctgt gcgagggaac attcggcaaa
    20401 cgaggtcgcc atgccgataa atgcggcaac ccacatccag aagacggctc caggtccacc
    20461 ggcggtaatt gccagcgcaa cgccggccag gttgccgcta cccacgcgcg ctgcaagact
    20521 ggtacacaat gactgaaatg aggttaaacc gcctggctgt ggatgaatgc tatttttaag
    20581 acttttgcca aactggcgga tgtagcgaaa ctgcacaaat ccggtgcgaa aagtgaacca
    20641 acaacctgcg ccgaagagca ggtaaatcat taccgatccc caaaggacgc tgttaataaa
    20701 ggagaaaaaa tctggcatgc atatccctct tattgccggt cgcgatgact ttcctgtgta
    20761 aacgttacca attgtttaag aagtatatac gctacgaggt acttgataac ttctgcgtag
    20821 catacatgag gttttgtata aaaatggcgg gcgatatcaa cgcagtgtca gaaatccgaa
    20881 acagtctcgc ctggcgataa ccgtcttgtc ggcggttgcg ctgacgttgc gtcgtgatat
    20941 catcagggca gaccggttac atccccctaa caagctgttt aaagagaaat actatcatga
    21001 cggacaaatt gacctccctt cgtcagtaca ccaccgtagt ggccgacact ggggacatcg
    21061 cggcaatgaa gctgtatcaa ccgcaggatg ccacaaccaa cccttctctc attcttaacg
    21121 cagcgcagat tccggaatac cgtaagttga ttgatgatgc tgtcgcctgg gcgaaacagc
    21181 agagcaacga tcgcgcgcag cagatcgtgg acgcgaccga caaactggcc gtaaatattg
    21241 gtctggaaat cctgaaactg gttccgggcc gtatctcaac tgaagttgat gcgcgtcttt
    21301 cctatgacac cgaagcgtca attacgaaag caaaacgcct gatcaaactc tacaacgatg
    21361 caggtattag caacgatcgt attctgatca aactggcttc tacctggcag ggtatccgtg
    21421 ctgcggaaca gctggaaaaa gaaggcatca actgtaacct gaccctgctg ttctccttcg
    21481 ctcaggctcg tgcttgtgcg gaagcgggcg tgttcctgat ctcgccgttt gttggccgta
    21541 ttcttgactg gtacaaagcg aataccgata agaaagagta cgctccggca gaagatccgg
    21601 gcgtggtttc tgtatctgaa atctaccagt actacaaaga gcacggttat gaaaccgtgg
    21661 ttatgggcgc aagcttccgt aacatcggcg aaattctgga actggcaggc tgcgaccgtc
    21721 tgaccatcgc accggcactg ctgaaagagc tggcggagag cgaaggggct atcgaacgta
    21781 aactgtctta cactggtgaa gtgaaagcgc gtccggcgcg tatcactgag tccgagttcc
    21841 tgtggcagca caaccaggat ccaatggcag tagataaact ggcggaaggt atccgtaagt
    21901 ttgctgttga ccaggaaaaa ctggaaaaaa tgatcggcga tctgctgtaa tcattcttag
    21961 cgtgaccggg aagtcggtca cgctacctct tctgaaacct gtctgtcact cccttcgcag
    22021 tgtatcattc tgtttaacga gactgtttaa acggaaaaat cttgatgaat actttacgta
    22081 ttggcttagt ttccatctct gatcgcgcat ccagcggcgt ttatcaggat aaaggcatcc
    22141 ctgcgctgga agaatggctg acatcggcgc taaccacgcc gtttgaactg gaaacccgct
    22201 taatccccga tgagcaggcg atcatcgagc aaacgttgtg tgagctggtg gatgaaatga
    22261 gttgccatct ggtgctcacc acgggcggaa ctggcccggc gcgtcgtgac gtaacgcccg
    22321 atgcgacgct ggcagtagcg gaccgcgaga tgccaggctt tggtgaacag atgcgccaga
    22381 tcagcctgca ttttgtacca actgcgatcc tttcgcgtca ggtgggggtg attcgcaaac
    22441 aggcgctgat ccttaactta cccggtcaac cgaagtctat taaagagacg ctggaaggtg
    22501 tgaaggacgc tgagggtaac gttgtggtgc acggtatttt tgccagcgta ccgtactgca
    22561 ttcagttgct ggaagggcca tacgttgaaa cggcaccgga agtggttgca gcattcagac
    22621 cgaagagtgc aagacgcgaa gttagcgaat aaaaaaatcc ccccaagcgg ggggatcaga
    22681 ctgatgacaa acgcaaaact gcctgatgcg ctacgcttat caggcctacg atcttcttgc
    22741 aatatattga atttgcacga ttttgtaggc cggataaggc gttcacgccg catccggcat
    22801 aaacaaagcg cacgttgtta acaatcttat cccttcgagc ggggggagct taacaattag
    22861 tgagactcac caatcggcag aacggtgcga ccaaactgct cgttcagcac ttcacccatc
    22921 gccagataga ttgcactggc accgcagata agaccaatcc agccggcaaa gtggatgatc
    22981 gcggcgttac cggcaatgtt accgatcgcc agcagggcaa acagcacggt caggctaaag
    23041 aaaacgaatt gcagaacgcg tgcgcctttc agcgtgccga agaacataaa cagcgtaaat
    23101 atgccccaca gacccaggta gacaccaagg aactgtgcat ttggcgcatc ggtcagcccc
    23161 agtttcggca tcagcagaat tgcaaccagc gtcagccaga aagaaccgta agaggtgaat
    23221 gcggttaaac cgaaagtgtt gccttttttg tactccagca gaccggcaaa aatttgcgcg
    23281 atgccgccgt agaaaatgcc catggcaaga ataataccgt ccagagcaaa ataacccacg
    23341 ttgtgcaggt taagcagaat ggtggtcatg ccgaagccca tcaggcccag cggtgccgga
    23401 ttagccaact tagtgttgcc cataattcct caaaaatcat catcgaatga atggtgaaat
    23461 aatttccctg aataactgta gtgttttcag ggcgcggcat aataatcagc cagtggggca
    23521 gtgtctacga tcttttgagg ggaaaatgaa aattttcccc ggtttccggt atcagacctg
    23581 agtggcgcta accatccggc gcaggcaggc gatttgcagt acggctggaa ttgtcacgcg
    23641 ataggcactg ccgctgaccg ctttaacccc atttaatgct gcgcccacag ggcctcccag
    23701 acccgcgccg cgcagcaaac catgcccaag cacgctcatt gctgcgtggg tgcgtaaaat
    23761 gcgggtcaat tggctggaaa gcaaatgcga cacacctttt gccaataatt tgtctttcat
    23821 cagcagcggc agcagctctt ccagctcatt caccctggca tcgaccgcgt gcagaaactc
    23881 ctgcttatgt tcctcgtcca ttttcttcca ggtattacgc agaaattgtt ccagtaactg
    23941 ttgctcaatt tcaaacgtag acatctcttt gtcggctttc agcttcaatc gctttgaaac
    24001 atcgagcaaa atggcgcgat acaatttacc gtgtccgcgc agtttgttgg cgatactatc
    24061 gccaccaaaa tgctgtaatt ctccggcaat cagctgccag ttgcggcggt gtcgctcggg
    24121 atgtccttcc atcgatttaa acagttcgtt gcgcatcagt acgctggaga ggcgagtttt
    24181 gcctttttca ttatgggtga gcagccgggc gaaatttgcc aactgttcct cactacaatg
    24241 ctggagaaaa tccagatctg aatcattcag gtaattaaca ttcatttttt gtggtttcta
    24301 tattctggcg ttagtcgtcg ccgataattt tcagcgtggc catatccgat gagttcactg
    24361 tatgaccgga aaaggtgatt tttgagacgc agcgtttgtt gtcgttatcg ctgttaatgt
    24421 tgatccagtc agtggtttgc ccttctttta tttctgaagg aatattcagg gtctgactgg
    24481 cgctacgggt ggctttgaaa taaatcgatg caccgcttaa ctgcaaatcg ccatggtcgg
    24541 ctgagagttg tatgcgttta acaatgcgac aaacaggaag tttcagcgcc agatcgttgg
    24601 tttcgtttcg cggcattgca atgacgccga ggattttatg gtcgtttgcc tgcgccgtgc
    24661 agcacagcat caggctaatc gccaggctgg cggaaatcgt aataacggat ttcataagga
    24721 ttctcttggt ggggaggggt aaggggatga aactagttta ctgctgataa agagaagttt
    24781 caggtacgta atattttctt tttattacaa ttttttgatg aatgccttgg ctgcgattca
    24841 ttctttatat gaataaaatt gttgtcagtt tcacgtcttg tcctgccata tcgcgaaatt
    24901 tctgcgcaaa agcacaaaaa atttttgcat ctcccccttg atgacgtggg ttacgacccc
    24961 atttagtagt caaccgcagt gagtgagtct gcaaaaaaat gaaattgggc agttgaaacc
    25021 agacgtttcg cccctattac agactcacaa ccacatgatg accgaatata tagtggagac
    25081 gtttagatgg gtaaaataat tggtatcgac ctgggtacta ccaactcttg tgtagcgatt
    25141 atggatggca ccactcctcg tgtactggag aacgccgaag gcgatcgcac cacgccttct
    25201 atcattgcct atacccagga tggtgaaact ctggttggtc agccggctaa acgtcaggca
    25261 gtgacgaacc cgcaaaacac cctgtttgcg attaaacgcc tgattggccg ccgcttccag
    25321 gacgaagaag tacagcgtga tgtttccatc atgccgttca aaattattgc tgctgataac
    25381 ggcgacgcat gggtcgaagt taaaggccag aaaatggcac cgccgcagat ctctgctgaa
    25441 gtgctgaaaa aaatgaagaa aaccgctgaa gattacctgg gtgaaccggt aactgaagct
    25501 gttattaccg taccggcata ctttaacgat gctcagcgtc aggcaaccaa agacgcaggc
    25561 cgtatcgctg gtctggaagt aaaacgtatc atcaacgaac cgaccgcagc tgcgctggct
    25621 tacggtctgg acaaaggtac tggcaaccgt actatcgcgg tttatgacct gggtggtggt
    25681 actttcgata tttccattat cgaaatcgac gaggttgacg gcgaaaaaac cttcgaagtt
    25741 ctggcaacca acggtgatac ccacctgggt ggtgaagact tcgacagtcg tctgatcaac
    25801 tatctggttg aagaattcaa gaaagatcag ggcattgacc tgcgcaacga tccgctggca
    25861 atgcagcgcc tgaaagaagc ggcagaaaaa gcgaaaatcg aactgtcttc cgctcagcag
    25921 accgacgtta acctgccgta catcactgca gacgcgaccg gtccgaaaca catgaacatc
    25981 aaagtgactc gtgcgaaact ggaaagcctg gttgaagatc tggtaaaccg ttccattgag
    26041 ccgctgaaag ttgcactgca ggacgctggc ctgtccgtat ctgatatcga cgacgttatc
    26101 ctcgttggtg gtcagactcg tatgccaatg gttcagaaga aagttgctga attctttggt
    26161 aaagagccgc gtaaagatgt taacccggac gaagctgtag ccatcggtgc tgctgttcag
    26221 ggtggtgttc tgactggtga cgtaaaagac gtactgctgc tggacgttac cccgctgtct
    26281 ctgggtatcg aaaccatggg cggtgtgatg accacgctga tcgcgaaaaa caccactatc
    26341 ccgaccaagc acagccaggt gttctctacc gctgaagaca accagtctgc ggtaaccatc
    26401 catgtgctgc agggtgaacg taaacgtgcg gctgataaca aatctctggg tcagttcaac
    26461 ctggatggta tcaacccggc accgcgcggc atgccgcaga tcgaagttac cttcgatatc
    26521 gatgctgacg gtatcctgca cgtttccgcg aaagacaaaa acagcggtaa agagcagaag
    26581 atcactatca aggcttcttc tggtctgaac gaagatgaaa tccagaaaat ggtacgcgac
    26641 gcagaagcta acgccgaagc tgaccgtaag tttgaagagc tggtacagac tcgcaaccag
    26701 ggcgaccatc tgctgcacag cacccgtaag caggttgaag aagcaggcga caaactgccg
    26761 gctgacgaca aaactgctat cgagtctgcg ctgactgcac tggaaactgc tctgaaaggt
    26821 gaagacaaag ccgctatcga agcgaaaatg caggaactgg cacaggtttc ccagaaactg
    26881 atggaaatcg cccagcagca acatgcccag cagcagactg ccggtgctga tgcttctgca
    26941 aacaacgcga aagatgacga tgttgtcgac gctgaatttg aagaagtcaa agacaaaaaa
    27001 taatcgccct ataaacgggt aattatactg acacgggcga aggggaattt cctctccgcc
    27061 cgtgcattca tctaggggca aatcaaaaaa gatggctaag caagattatt acgagatttt
    27121 aggcgtttcc aaaacagcgg aagagcgtga aatcaaaaag gcctacaaac gcctggccat
    27181 gaaataccac ccggaccgta accagggtga caaagaggcc gaggcgaaat ttaaagagat
    27241 caaggaagct tatgaagttc tgaccgactc gcaaaaacgt gcggcatacg atcagtatgg
    27301 tcatgctgcg tttgagcaag gcggcatggg cggcggcggt tttggcggcg gcgcagactt
    27361 cagcgatatt tttggtgacg ttttcggcga tatttttggc ggcggacgtg gtcgtcaacg
    27421 tgcggcgcgc ggtgctgatt tacgctataa catggagctc accctcgaag aagctgtacg
    27481 tggcgtgacc aaagagatcc gcattccgac tctggaagag tgtgacgttt gccacggtag
    27541 cggtgcaaaa ccaggtacac agccgcagac ctgtccgacc tgtcatggtt ctggccaggt
    27601 gcagatgcgc cagggtttct ttgccgtgca gcagacctgt ccacactgtc agggccgcgg
    27661 tacgctgatc aaagatccgt gcaacaaatg tcatggtcat ggtcgtgttg agcgcagcaa
    27721 aacgctgtcc gttaaaatcc cggcaggggt ggacactgga gaccgcatcc gtcttgcggg
    27781 cgaaggtgaa gcgggtgaac acggcgcacc ggcaggcgat ctgtacgttc aggttcaggt
    27841 taaacagcac ccgattttcg agcgtgaagg caacaacctg tattgcgaag tcccgatcaa
    27901 cttcgctatg gcggcgctgg gtggtgaaat cgaagtaccg acccttgatg gtcgcgtcaa
    27961 actgaaagtg cctggcgaaa cccagaccgg taagctgttc cgtatgcgcg gtaaaggcgt
    28021 caagtctgtc cgcggtggcg cacagggtga tttgctatgc cgcgttgttg tcgaaacacc
    28081 ggtaggtttg aacgagaagc agaaacagct gctgcaagag ctgcaagaaa gctttggtgg
    28141 cccaaccggc gagcacaaca gcccgcgttc aaagagcttc tttgatggcg tgaagaagtt
    28201 ttttgacgac ctgactcgct aacctcccca aaagcctgcc cgtgggcagg ccagggtaaa
    28261 aaccagggtg cgttgaagat atgcgagcac ctgtaaagtg gcggggatca ctccccgccg
    28321 ttgctcttac tcggattcgt aagccgtgaa aacagcaacc tccgtctggc cagttcggat
    28381 gtgaacctca cagaggtctt ttctcgttac cagcgccgcc actacggcgg tgatacagat
    28441 gacgatcagg gcgacaatca tcgccttatg ctgcttcatt gctctcttct ccttgacctt
    28501 tcggtcagta agaggcactc tacatgtgtt ctgcatatag ggggcctcgg gttgatggta
    28561 aaatatcact cggggctttt ctctatctgc cgttcagcca atgcctgaga cagacagcct
    28621 caagcacccg ccgctattat actgcgcttt taattcactt ctgttttatc aattcttttt
    28681 ctgaagacgc ctcgcatttt tgcggaataa tatttcaata catcaattta tttaacttaa
    28741 atatatccag gaattcacaa aaaaaggtgt tttcctctat caaaagtgaa tggctaaagg
    28801 aaattaatta ttaaaggtta ctgtaatact atagagagac aaagaaatac gttcagcagc
    28861 caatgctaag ttcatcatga aaaatgccta taaatcaaag ggtgatgttt aaaacagaca
    28921 cattttctga aacacgatat ccctccgccc gttcgcaaaa gtaatctgcg atcttcatca
    28981 cgcttcgata gtcatcttta aatatactgg cctggtcatt cttaataaaa atgaattgtc
    29041 tggtgtatta attcctatgg aatcataaaa acaatgaatg gagaacatat tatatgtctg
    29101 ccaaaagacg acttcttatt gcgtgtaccc tgataacagc tatctatcat tttcctgcat
    29161 attcttcatt agaatataaa ggaacctttg gttcaataaa tgcgggttat gcagactgga
    29221 atagtggatt tgtaaacact caccgtggtg aagtatggaa agtgactgcg gattttgggg
    29281 taaattttaa agaagcagaa ttttactcat tttatgaaag taatgtactc aatcatgctg
    29341 tagcagggag aaatcatacg gtttcagcaa tgacgcatgt cagactcttt gactctgata
    29401 tgacattctt tggcaaaatt tatggccaat gggataactc atggggtgac gatctggaca
    29461 tgttttatgg attcggttac ctcggctgga acggcgagtg gggctttttt aaaccgtata
    29521 ttggattgca taatcaatct ggtgactacg tatcagctaa atatggtcaa acgaatggtt
    29581 ggaatggtta tgttgttggc tggacagcag tattaccatt tacgttattt gacgaaaaat
    29641 ttgttttatc taactggaat g
//
LOCUS       DADSYM010000048        29214 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2415
            SAMN08039579-rid11723173.denovo.048, whole genome shotgun sequence.
ACCESSION   DADSYM010000048 DADSYM010000000
VERSION     DADSYM010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039579
            Sequence Read Archive: SRR6362533
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 29214)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29214)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980162.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 03:39:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,484
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 4,347
            CDSs (with protein)               :: 4,347
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 43 of 85
            Pseudo Genes (incomplete)         :: 37 of 85
            Pseudo Genes (internal stop)      :: 19 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29214
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039579-rid11723173.denovo.048"
                     /strain="LGE2415"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8302 N 95.9628 W"
                     /collection_date="2015-08-24"
     gene            13..1356
                     /locus_tag="J1Y83_001787"
     CDS             13..1356
                     /locus_tag="J1Y83_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310521.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HBA7629938.1"
                     /translation="MASMGTQKLKAQGFFVFSLLLTLILFCITTLYNENTNVKLIPQM
                     NYLMVVVALFFLNAVIFLFMLMKYFTNKQILPTLILSLAFLSGLIYLVETIVIIHKPI
                     NGSTLIQTKSNDVSIFYIFRQLSFICLTSLALFCYGKDNILDNNKKKTGILLLALIPF
                     LVFPLLAHNLSSYNADYSLYVVDYCPDNHTATWGINYTKILVCLWAFLLFFIIMRTRL
                     ASELWPLIALLCLASLCCNLLLLTLDEYNYTIWYISRGIEVSSKLFVVSFLIYNIFQE
                     LQLSSKLAVHDVLTNIYNRRYFFNSVESLLSRPVVKDFCVMLVDINQFKRINAQWGHR
                     VGDKVLVSIVDIIQQSIRPDDILARLEGEVFGLLFTELNSAQAKIIAERMRKNVELLT
                     GFSNRYDVPEQMTISIGTVFSTGDTHNISLVMTEADKALREAKSEGGNKVIIHHI"
     gene            1537..3027
                     /locus_tag="J1Y83_001788"
     CDS             1537..3027
                     /locus_tag="J1Y83_001788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416300.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HBA7629939.1"
                     /translation="MKLHHRMLRHFIAASVIVLTSSFLIFELVASDRAMSAYLRYIVQ
                     RADSSFLYDKYQNQSIAAHVMRALAAEQSEVPPEQRRAICEAFESANNTHGLNLTAHK
                     YPGLRGTLQTASTDCDTIVEAAALLPAFDQAVEGNRHQDDYGSGLGMAEEKFHYYLDL
                     NDRYVYFYEPVNVEYFAMNNWSFLQSGSIGIDRKDIEKVFTGRTVLSSIYQDQRTKQN
                     VMSLLTPVYVAGQLKGIVLLDINKNNLRNIFYTHDRPLLWRFLNVTLTDTDSGRDIII
                     NQSEDNLFQYVSYVHDLPGGIRVSLSIDILYFITSSWKSVLFWILTALILLNMVRMHF
                     RLYQNVSRENISDAMTGLYNRKILTPELEQRLQKLVQSGSSVMFIAIDMDKLKQINDT
                     LGHQEGDLAITLLAQAIKQSIRKSDYAIRLGGDEFCIILVDSTPQIAAQLPERIEKRL
                     QHIAPQKEIGFSSGIYAMKENDTLHDAYKASDERLYVNKQNKNSRS"
     gene            3070..3573
                     /gene="yeaK"
                     /locus_tag="J1Y83_001789"
     CDS             3070..3573
                     /gene="yeaK"
                     /locus_tag="J1Y83_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mischarged aminoacyl-tRNA deacylase"
                     /protein_id="HBA7629940.1"
                     /translation="MTEMAKGSVTHQRLIALLSQEGANFRVVTHEAVGKCEAVSEIRG
                     TALGQGAKALVCKVKGNGVNQHVLAILAADQQADLSQLASHIGGLRASLASPAEVDEL
                     TGCVFGAIPPFSFHPKLKLVADPLLFERFDEIAFNAGMLDKSVILKTADYLRIAQPEL
                     VNFRRTA"
     gene            complement(3574..3678)
                     /locus_tag="J1Y83_001790"
     CDS             complement(3574..3678)
                     /locus_tag="J1Y83_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416302.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA7629941.1"
                     /translation="MNDQMFVETLIITSSFFAIAAVLVLSVLLIERTG"
     gene            3848..4294
                     /locus_tag="J1Y83_001791"
     CDS             3848..4294
                     /locus_tag="J1Y83_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF441 domain-containing protein"
                     /protein_id="HBA7629942.1"
                     /translation="MFDVTLLILLGLAALGFISHNTTVAVSILVLIIVRVTPLSTFFP
                     WIEKQGLSIGIIILTIGVMAPIASGTLPPSTLIHSFLNWKSLVAIAVGVIVSWLGGRG
                     VTLMGSQPQLVAGLLVGTVLGVALFRGVPVGPLIAAGLVSLIVGKQ"
     gene            complement(4251..5072)
                     /locus_tag="J1Y83_001792"
     CDS             complement(4251..5072)
                     /locus_tag="J1Y83_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBA7629943.1"
                     /translation="MMHRLNLNGYEPDRHHEAAVAFCIHAGTDELTSPVHQHRKGQLI
                     LALHGAITCTVENALWMVPPQYAVWIPGGVEHSNQVTANAELCFLFIEPSAVTMPTTC
                     CTLKISPLCRELILTLANRTTTQRAEPMTRRLIQVLFDELPQQPQQQLHLPVSSHPKI
                     RTMVEMMAKEPVEWGSLGQWAGFFAMSERNLARLIVKETGLSFRQWRQQLQLIMALQG
                     LVKGDTVQKVAHTLGYDSTTAFITMFKKGLGQTPGRYIAGLTTVSPQSAKPDPRQ"
     gene            5169..6350
                     /gene="nimT"
                     /locus_tag="J1Y83_001793"
     CDS             5169..6350
                     /gene="nimT"
                     /locus_tag="J1Y83_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-nitroimidazole transporter"
                     /protein_id="HBA7629944.1"
                     /translation="MTCSTSLSGKNRIFLIAGILMIATTLRVTFTGAAPLLDTIRSAY
                     SLTTAQTGLLTTLPLLAFALISPLAAPVARRFGMERSLFAALLLICAGIAIRSLPSPY
                     LLFGGTAIIGGGIALGNVLLPGLIKRDFPHSVARLTGAYSLTMGAAAALGSAMVVPLA
                     LNGFGWQGALLMLMCFPLLALFLWLPQWRSQQHANLSTSRALHTRGIWRSPLAWQVTL
                     FLGINSLVYYVIIGWLPAILISHGYSEAQAGSLHGLLQLATAAPGLLIPLFLHHVKDQ
                     RGIAAFVALMCAVGAVGLCFMPAHAITWTLLFGFGSGATMILGLTFIGLRASSAHQAA
                     ALSGMAQSVGYLLAACGPPLMGKIHDANGDWSVPLMGVAILSLLMAIFGLCAGRDKEI
                     R"
     gene            6405..6752
                     /locus_tag="J1Y83_001794"
     CDS             6405..6752
                     /locus_tag="J1Y83_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HBA7629945.1"
                     /translation="MNIQCKRVYDPAEQSDGYRVLVDRLWPRGIKKTDLALDEWDKEI
                     TPSTELRKAFHGEVVDFATFREQYLAELAQHEQEGKRLANIAKKQPLTLLYSAKNTTQ
                     NHALVLADWLRSL"
     gene            complement(6774..7028)
                     /gene="yoaF"
                     /locus_tag="J1Y83_001795"
     CDS             complement(6774..7028)
                     /gene="yoaF"
                     /locus_tag="J1Y83_001795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF333 domain-containing lipoprotein YoaF"
                     /protein_id="HBA7629946.1"
                     /translation="MKIISFVLPCLLVLAGCSTPSQPEAPKPPQIGMANPASVYCQQK
                     GGTLIPVQTAQGVSNNCKLPGGETIDEWTLWRRDHPAGEK"
     gene            7211..8236
                     /gene="dgcP"
                     /locus_tag="J1Y83_001796"
     CDS             7211..8236
                     /gene="dgcP"
                     /locus_tag="J1Y83_001796"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416308.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcP"
                     /protein_id="HBA7629947.1"
                     /translation="MSDQIIARVSQSLAKEQSLESLVRQLLEMLEMVTDMESTYLTKV
                     DVEARLQHIMFARNSQKMHIPENFTVSWDYSLCKRAIDENCFFSDEVPDRWGDCIAAR
                     NLGITTFLSTPIHLPDGSFYGTLCAASSEKRQWSERAEQVLQLFAGLIAQYIQKEALV
                     EQLREANAALIAQSYTDSLTGLPNRRAIFENLTTLFSLARHLNHKIMIAFIDLDNFKL
                     INDRFGHNSGDLFLIQVGERLNTLQQNGEVIGRLGGDEFLVVSLNNENADISSLRERI
                     QQQIRGEYHLGDVDLYYPGASLGIVEVDPETTDADSALHAADIAMYQEKKHKQKTPFV
                     THSALHS"
     gene            8269..8367
                     /gene="yoaK"
                     /locus_tag="J1Y83_001797"
     CDS             8269..8367
                     /gene="yoaK"
                     /locus_tag="J1Y83_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoaK family small membrane protein"
                     /protein_id="HBA7629948.1"
                     /translation="MRIGIIFPVVIFITAVVFLAWFFIGGYAAPGA"
     gene            8370..8444
                     /gene="yoaJ"
                     /locus_tag="J1Y83_001798"
     CDS             8370..8444
                     /gene="yoaJ"
                     /locus_tag="J1Y83_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YoaJ"
                     /protein_id="HBA7629949.1"
                     /translation="MKKTTIIMMGVAIIVVLGTELGWW"
     gene            complement(8503..8751)
                     /locus_tag="J1Y83_001799"
     CDS             complement(8503..8751)
                     /locus_tag="J1Y83_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HBA7629950.1"
                     /translation="MGILSWIIFGLIAGILAKWIMPGKDGGGFFMTILLGIVGAVVGG
                     WISTLFGFGKVDGFNFGSFVVAVIGAIVVLFIYRKIKS"
     gene            complement(8899..9081)
                     /locus_tag="J1Y83_001800"
     CDS             complement(8899..9081)
                     /locus_tag="J1Y83_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1869 domain-containing protein"
                     /protein_id="HBA7629951.1"
                     /translation="MGKATYTVTVTNNSNGVSVDYETETPMTLLVPEVAAEVIKDLVN
                     TVRSYDTENEHDVCGW"
     gene            complement(9085..9444)
                     /gene="yeaR"
                     /locus_tag="J1Y83_001801"
     CDS             complement(9085..9444)
                     /gene="yeaR"
                     /locus_tag="J1Y83_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1971 domain-containing protein"
                     /protein_id="HBA7629952.1"
                     /translation="MLQIPQNYIHTRSTPFWNKQTAPAGIFERHLDKGTRPGVYPRLS
                     VMHGAVKYLGYADEHSAEPDQVILIEAGQFAVFPPEKWRNIEAMTDDTYFNIDFFVAP
                     EVLMEGAQQRKVIHNGK"
     gene            complement(9616..10254)
                     /gene="leuE"
                     /locus_tag="J1Y83_001802"
     CDS             complement(9616..10254)
                     /gene="leuE"
                     /locus_tag="J1Y83_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine efflux protein LeuE"
                     /protein_id="HBA7629953.1"
                     /translation="MFAEYGVLNYWTYLVGAIFIVLVPGPNTLFVLKNSVSSGMKGGY
                     LAACGVFIGDAVLMFLAWAGVATLIKTTPILFNIVRYLGAFYLLYLGSKILYATLKGK
                     NSETKSDEPQYGAIFKRALILSLTNPKAILFYVSFFVQFIDVNAPHTGISFFILATTL
                     ELVSFCYLSFLIISGAFVTQYIRTKKKLAKVGNSLIGLMFVGFAARLATLQS"
     gene            complement(10381..10548)
                     /locus_tag="J1Y83_001803"
                     /pseudo
     CDS             complement(10381..10548)
                     /locus_tag="J1Y83_001803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310535.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            10537..10707
                     /locus_tag="J1Y83_001804"
     CDS             10537..10707
                     /locus_tag="J1Y83_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000206915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dehydrogenase"
                     /protein_id="HBA7629954.1"
                     /translation="MTTGGKQPGDIDFYVVRKNTEGEYSSPGGRVNEGTENEVVIQES
                     VFTRRGVERILR"
     gene            complement(10797..11912)
                     /gene="rnd"
                     /locus_tag="J1Y83_001805"
     CDS             complement(10797..11912)
                     /gene="rnd"
                     /locus_tag="J1Y83_001805"
                     /EC_number="3.1.13.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310540.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease D"
                     /protein_id="HBA7629955.1"
                     /translation="MITTDDALASLCEAVRAFPAIALDTEFVRTRTYYPQLGLIQLFD
                     GEHLALIDPLGITDWSPLKAILRDPSITKFLHAGSEDLEVFLNVFGELPQPLIDTQIL
                     AAFCGRPMSWGFASMVEEYSGLTLDKSESRTDWLARPLTERQCEYAAADVWYLLPITA
                     KLMVETEASGWLPAALDECRLMQMRRQEVVAPEDAWRDITNAWQLRTRQLACLQLLAD
                     WRLRKARERDLAVNFVVREEHLWSVARYMPGSLGELDSLGLSGSEIRFHGKTLLALVE
                     KAQALPEEALPQPMLNLMDMPGYRKAFKAIKSLITDVSETHKISAELLASRRQINQLL
                     NWHWKLKPQNNLPELISGWRGELMAEALHNLLQEYPQ"
     gene            complement(11994..13679)
                     /gene="fadD"
                     /locus_tag="J1Y83_001806"
     CDS             complement(11994..13679)
                     /gene="fadD"
                     /locus_tag="J1Y83_001806"
                     /EC_number="6.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain-fatty-acid--CoA ligase FadD"
                     /protein_id="HBA7629956.1"
                     /translation="MKKVWLNRYPADVPTEINPDRYQSLVDMFEQSVARYADQPAFVN
                     MGEVMTFRKLEERSRAFAAYLQQGLGLKKGDRVALMMPNLLQYPVALFGILRAGMIVV
                     NVNPLYTPRELEHQLNDSGASAIVIVSNFAHTLEKVVDKTAVQHVILTRMGDQLSTAK
                     GTVVNFVVKYIKRLVPKYHLPDAISFRSALHNGYRMQYVKPELVPEDLAFLQYTGGTT
                     GVAKGAMLTHRNMLANLEQVNATYGPLLHPGKELVVTALPLYHIFALTINCLLFIELG
                     GQNLLITNPRDIPGLVKELAKYPFTAITGVNTLFNALLNNKEFQQLDFSSLHLSAGGG
                     MPVQQVVAERWVKLTGQYLLEGYGLTECAPLVSVNPYDIDYHSGSIGLPVPSTEAKLV
                     DDDDNEVPPGQPGELCVKGPQVMLGYWQRPDATDEIIKNGWLHTGDIAVMDEEGFLRI
                     VDRKKDMILVSGFNVYPNEIEDVVMQHPGVQEVAAVGVPSGSSGEAVKIFVVKKDPSL
                     TEESLVTFCRRQLTGYKVPKLVEFRDELPKSNVGKILRRELRDEARGKVDNKA"
     gene            complement(13884..14465)
                     /gene="yeaY"
                     /locus_tag="J1Y83_001807"
     CDS             complement(13884..14465)
                     /gene="yeaY"
                     /locus_tag="J1Y83_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Slp family lipoprotein YeaY"
                     /protein_id="HBA7629957.1"
                     /translation="MAVQKNVIKGILAGTFALMLSGCVTVPDAIKGSSPTPQQDLVRV
                     MSAPQLYVGQEARFGGKVVAVQNQQGKTRLEIATVPLDSGARPTLGEPSRGRIYADVN
                     GFLDPVDFRGQLVTVVGPITGAVDGKIGNTPYKFMVMQVTGYKRWHLTQQVIMPPQPI
                     DPWFYGGRGWPYGYGGWGWYNPGPARVQTVVTE"
     gene            complement(14505..15200)
                     /gene="tsaB"
                     /locus_tag="J1Y83_001808"
     CDS             complement(14505..15200)
                     /gene="tsaB"
                     /locus_tag="J1Y83_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HBA7629958.1"
                     /translation="MRILAIDTATEACSVALWNDGTVNAHFELCPREHTQRILPMVQD
                     ILTTSGTSLTDINALAYGRGPGSFTGVRIGIGIAQGLALGAELPMIGVSTLMTMAQGA
                     WRKNGATRVLAAIDARMGEVYWAEYQRDENGIWHGEETEAVLKPELVHERMQQLSGEW
                     VTVGTGWQAWPDLGKESGLVLLDGEVLLPAAEDMLPIACQMFAEGKTVAVEHAEPVYL
                     RNNVAWKKLPGKE"
     gene            complement(15258..17168)
                     /locus_tag="J1Y83_001809"
     CDS             complement(15258..17168)
                     /locus_tag="J1Y83_001809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HBA7629959.1"
                     /translation="MTDDFAPDGQLAKAIPGFKPREPQRQMAVAVTQAIEKGQPLVVE
                     AGTGTGKTYAYLAPALRAKKKVIISTGSKALQDQLYSRDLPTVSKALKYTGNVALLKG
                     RSNYLCLERLEQQALAGGDLPVQILSDVILLRSWSNQTVDGDISTCVSVAEDSQAWPL
                     VTSTNDNCLGSDCPMYKDCFVVKARKKAMDADVVVVNHHLFLADMVVKESGFGELIPE
                     ADVMIFDEAHQLPDIASQYFGQSLSSRQLLDLAKDISIAYRTELKDTQQLQKCADRLA
                     QSAQDFRLQLGEPGYRGNLRELLANPQIQRAFLLLDDTLELCYDVAKLSLGRSALLDA
                     AFERATLYRTRLKRLKEINQPGYSYWYECTSRHFTLALTPLSVADKFKELMAQKTGSW
                     IFTSATLSVNDDLHHFTSRLGIEQAESLLLPSPFDYSRQALLCVPRNLPQTNQPGSAR
                     QLAAMLRPIIEANNGRCFMLCTSHAMMRDLAEQFRATMTLPVLLQGETSKGQLLQQFV
                     SAGNALLVATSSFWEGVDVRGDTLSLVIIDKLPFTSPDDPLLKARMEDCRLRGGDPFD
                     EVQLPDAVITLKQGVGRLIRDADDRGVLVICDNRLVMRPYGATFLASLPPAPRTRDIA
                     RAVRFLAIPSSR"
     gene            17300..17644
                     /locus_tag="J1Y83_001810"
     CDS             17300..17644
                     /locus_tag="J1Y83_001810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416323.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HBA7629960.1"
                     /translation="MTIVRIDAEARWSDVVIHNNTLYYTGVPENLDADAFEQTANTLA
                     QIDAVLEKQGSNKSSILDATIFLADKNDFAAMNKAWDAWVVAGHAPVRCTVQAGLMNP
                     KYKVEIKIVAAV"
     gene            18006..18365
                     /locus_tag="J1Y83_001811"
     CDS             18006..18365
                     /locus_tag="J1Y83_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015912513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1889 family protein"
                     /protein_id="HBA7629961.1"
                     /translation="MQIKVIYSLIDNMVNFKDKNMPAVIDKALDFIGAMDVSAPTPSS
                     MNESTAKGIFKYLKELGVPASAADITARADQEGWNPGFTEKMVGWAKKMESGERIVIK
                     NPEYFSTYMQEELKALV"
     gene            complement(18485..18664)
                     /locus_tag="J1Y83_001812"
     CDS             complement(18485..18664)
                     /locus_tag="J1Y83_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoaH family protein"
                     /protein_id="HBA7629962.1"
                     /translation="MFAGLPSLTHEQQQKAVERIQELMAQGMSSGQAIALVAEELRAN
                     HSGERIVARFEDEDE"
     gene            18738..20099
                     /gene="pabB"
                     /locus_tag="J1Y83_001813"
     CDS             18738..20099
                     /gene="pabB"
                     /locus_tag="J1Y83_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component 1"
                     /protein_id="HBA7629963.1"
                     /translation="MKTLSPAVITLPWRQDAAEFYFSRLSHLPWAMLLHSGYADHPYS
                     RFDIVVAEPICTLTTFGKETVVCEGETSSTTTDDPLQVLQQVLDRADIHPAHNEDLPF
                     QGGALGLFGYDLGRRFESLPEIAEQDIVLPDMAVGIYDWALVVDHQRQTVSLLSHNDV
                     NARRAWLENQQFSLQEDFALTSDWQSNMTREQYGEKFRQVQEYLHSGDCYQVNLAQRF
                     HATYSGDEWQAFLQLNQANRAPFSAFLRLEQGAILSLSPERFILCDNSEIQTRPIKGT
                     LPRLPDPQEDSKQAEKLANSAKDRAENLMIVDLMRNDIGRVAVAGSVKVPELFVVEPF
                     PAVHHLVSTITAQLPEQLHASDLFRAAFPGGSITGAPKVRAMEIIDELEPHRRNAWCG
                     SIGYLSFCGNMDTSITIRTLTAINGQIYCSAGGGIVADSQEEAEYQETFDKVNKILRQ
                     LEK"
     gene            20103..20681
                     /locus_tag="J1Y83_001814"
     CDS             20103..20681
                     /locus_tag="J1Y83_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HBA7629964.1"
                     /translation="MEYRSLTLDDFLSRFQLLRPQINRETLNHRQAAVLIPIVRRPQP
                     GLLLTQRSIHLRKHAGQVAFPGGAVDDTDASVIAAALREAEEEVAIPPSAVEVIGVLP
                     PVDSVTGYQVTPVVGIIPPDLPYRASEDEVSAVFEMPLAQALHLGRYHPLDIYRRGDS
                     HRVWLSWYEQYFVWGMTAGIIRELALQIGVKP"
     gene            20865..22229
                     /gene="sdaA"
                     /locus_tag="J1Y83_001815"
     CDS             20865..22229
                     /gene="sdaA"
                     /locus_tag="J1Y83_001815"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HBA7629965.1"
                     /translation="MISLFDMFKVGIGPSSSHTVGPMKAGKQFVDDLVEKGLLDSVTR
                     VAVDVYGSLSLTGKGHHTDIAIIMGLAGNEPATVDIDSIPGFIRDVEERERLLLAQGR
                     HEVDFPRDNGMRFHNGNLPLHENGMQIHAYNGDEVVYSKTYYSIGGGFIVDEEHFGQD
                     AANEVSVPYPFKSATELLAYCNETGFSLSGLAMQNELALHSKKEIDEYFAHVWQTMQA
                     CIDRGMNTEGVLPGPLRVPRRASALRRMLVSSDKLSNDPMNVIDWVNMFALAVNEENA
                     AGGRVVTAPTNGACGIVPAVLAYYDHFIESVSPDIYTRYFMAAGAIGALYKMNASISG
                     AEVGCQGEVGVACSMAAAGLAELLGGSPEQVCVAAEIGMEHNLGLTCDPVAGQVQVPC
                     IERNAIASVKAINAARMALRRTSAPRVSLDKVIETMYETGKDMNAKYRETSRGGLAIK
                     VQCD"
     gene            22360..23958
                     /locus_tag="J1Y83_001816"
     CDS             22360..23958
                     /locus_tag="J1Y83_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416329.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HBA7629966.1"
                     /translation="MQKAQRIIKTYRRNRMIVCTICALVTLASTLSVRFISQRNLNQQ
                     RVVQFANHAVEELDKVLLPLQAGSEVLLPLIGLPCSVAHLPLRKQAAKLQTVRSIGLV
                     QDGTLYCSSIFGYRNVPVVDILAELPAPQPLLRLTTDRALIKGSPVLIQWTPAAGSSN
                     AGVMEMINIDLLTAMLLEPQLPQISSASLTVDSRHLLYGNGLVDSLPQPENNENYQVS
                     SQRFPFTINVNGPGATALAWHYLPTQLPLAVLLSLLVGYIAWLATAYRMSFSREINLG
                     LAQHEFELFCQPLLNARSQQCIGVEILLRWNNPRQGWISPDVFIPIAEEHHLIVPLTR
                     YVMAETIRQRHVFPMSSQFHVGINVAPSHFRRGVLIKDLNQYWFSAHPIQQLILEITE
                     RDALLDVDYRIARELHRKNVKLAIDDFGTGNSSFSWLETLRPDVLKIDKSFTAAIGSD
                     AVNSTVTDIIIALGQRLNIELVAEGVETQEQAKYLRSHGVHILQGYLYAQPMPLRDFP
                     KWLAGSQPPPARHNGHITPIMPLR"
     gene            complement(23962..25518)
                     /gene="yoaE"
                     /locus_tag="J1Y83_001817"
     CDS             complement(23962..25518)
                     /gene="yoaE"
                     /locus_tag="J1Y83_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM family cation transport protein YoaE"
                     /protein_id="HBA7629967.1"
                     /translation="MEFLMDPSIWAGLLTLVVLEIVLGIDNLVFIAILADKLPPKQRD
                     KARLLGLSLALIMRLGLLSLISWMVTLTKPLFTVMDFSFSGRDLIMLFGGIFLLFKAT
                     TELHERLENRDHDSGHGKGYASFWVVVTQIVILDAVFSLDAVITAVGMVNHLPVMMAA
                     VVIAMAVMLLASKPLTRFVNQHPTVVVLCLSFLLMIGLSLVAEGFGFHIPKGYLYAAI
                     GFSIIIEVFNQIARRNFIRHQSTLPLRARTADAILRLMGGKRQANVQHDADNPMPMPI
                     PEGAFAEEERYMINGVLTLASRSLRGIMTPRGEISWVDANLGVDEIREQLLSSPHSLF
                     PVCRGELDEIIGIVRAKELLVALEEGVDVAAIASASPAIIVPETLDPINLLGVLRRAR
                     GSFVIVTNEFGVVQGLVTPLDVLEAIAGEFPDADETPEIITDGDGWLVKGGTDLHALQ
                     QALDVEHLADDDDIATVAGLVISANGHIPRVGDVIDVGPLHITIIEANDYRVDLVRIV
                     KEQPAHDEDE"
     gene            25982..26953
                     /gene="manX"
                     /locus_tag="J1Y83_001818"
     CDS             25982..26953
                     /gene="manX"
                     /locus_tag="J1Y83_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIAB"
                     /protein_id="HBA7629968.1"
                     /translation="MTIAIVIGTHGWAAEQLLKTAEMLLGEQENVGWIDFVPGENAET
                     LIEKYNAQLAKLDTTKGVLFLVDTWGGSPFNAASRIVVDKEHYEVIAGVNIPMLVETL
                     MARDDDPSFDELVALAVETGREGVKALKAKPIEKAAPAPAAAAPKVAPTPAKPMGPND
                     YMVIGLARIDDRLIHGQVATRWTKETNVSRIIVVSDEVAADTVRKTLLTQVAPPGVTA
                     HVVDVAKMIRVYNNPKYAGERVMLLFTNPTDVERLVEGGVKITSVNVGGMAFRQGKTQ
                     VNNAVSVDEKDIEAFKKLNARGIELEVRKVSTDPKLKMMDLISKIDK"
     gene            27016..27816
                     /locus_tag="J1Y83_001819"
     CDS             27016..27816
                     /locus_tag="J1Y83_001819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HBA7629969.1"
                     /translation="MEITTLQIVLVFIVACIAGMGSILDEFQFHRPLIACTLVGIVLG
                     DMKTGIIIGGTLEMIALGWMNIGAAVAPDAALASIISTILVIAGHQSIGAGIALAIPL
                     AAAGQVLTIIVRTITVAFQHAADKAADNGNLTAISWIHVSSLFLQAMRVAIPAVIVAL
                     SVGTSEVQNMLNAIPEVVTNGLNIAGGMIVVVGYAMVINMMRAGYLMPFFYLGFVTAA
                     FTNFNLVALGVIGTVMAVLYIQLSPKYNRVAGAPAQAAGNNDLDNELD"
     gene            27829..28680
                     /locus_tag="J1Y83_001820"
     CDS             27829..28680
                     /locus_tag="J1Y83_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020804907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IID"
                     /protein_id="HBA7629970.1"
                     /translation="MVDTTQTTTEKKLTQSDIRGVFLRSNLFQGSWNFERMQALGFCF
                     SMVPAIRRLYPENNEARKQAIRRHLEFFNTQPFVAAPILGVTLALEEQRANGAEIDDG
                     AINGIKVGLMGPLAGVGDPIFWGTVRPVFAALGAGIAMSGSLLGPLLFFILFNLVRLA
                     TRYYGVAYGYSKGIDIVKDMGGGFLQKLTEGASILGLFVMGALVNKWTHVNIPLVVSR
                     ITDQTGKEHVTTVQTILDQLMPGLVPLLLTFACMWLLRKKVNPLWIIVGFFVIGIAGY
                     ACGLLGL"
     gene            28735..29193
                     /locus_tag="J1Y83_001821"
     CDS             28735..29193
                     /locus_tag="J1Y83_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF986 domain-containing protein"
                     /protein_id="HBA7629971.1"
                     /translation="MTITDLVLILFIAALLAFAIYDQFIMPRRNGPTLLAIPLLRRGR
                     IDSVIFVGLIVILIYNNVTNHGALITTWLLSALALMGFYIFWIRVPKIIFKQKGFFFA
                     NVWIEYSRIKAMNLSEDGVLVMQLEQRRLLIRVRNIDDLEKIYKLLVSTQ"
BASE COUNT         7225 a         7379 c         7105 g         7505 t
ORIGIN      
        1 gctctccttt ttatggcttc tatgggtaca caaaaattaa aagctcaagg cttttttgtt
       61 ttcagcttat tgctgacgtt aattttattt tgcattacta ccttatataa cgaaaacaca
      121 aatgtaaaac tcatcccaca gatgaattac ctgatggttg ttgtggcttt gtttttcctt
      181 aacgccgtca ttttcctttt catgttaatg aagtatttca ctaacaaaca aattttacca
      241 acactcattt taagccttgc atttttaagt ggccttatct atttagttga aaccattgta
      301 attatccata aaccaatcaa cggcagtaca ctgatccaga caaagtcgaa tgatgtttct
      361 attttctata ttttccgcca actcagtttc atttgtttaa cctcgctggc gctcttttgt
      421 tatgggaaag acaacatcct tgacaacaat aagaaaaaaa ctggcattct gttgctggcg
      481 ctgattcctt ttttagtttt tccccttctg gcacacaatc tgagcagtta taacgctgac
      541 tattctttgt atgtcgtcga ttactgtccg gacaaccata ctgcgacctg gggaatcaac
      601 tatacaaaaa tattggtttg tttgtgggca tttttactgt tctttattat catgcgtaca
      661 cgattagcca gcgaactatg gccgttaata gcattattat gtctggcatc gctatgctgc
      721 aacttacttc tactgactct ggatgagtat aattacacca tctggtatat cagtcgcggg
      781 attgaagttt ccagtaaact gtttgttgtg tcttttctga tttataacat ttttcaggaa
      841 ctgcaactct ccagcaaact ggcagttcat gatgtactga ccaatattta taatcggcgc
      901 tactttttca acagcgtaga gtcattattg tcgcgacctg ttgttaagga cttttgtgtt
      961 atgctggttg atattaatca gttcaaacgc atcaatgccc aatggggaca tcgtgtgggt
     1021 gataaagtgc tggtatcaat tgtcgatatt atccagcaaa gcatccgccc cgatgatatt
     1081 ttagcgcgac tggagggtga ggtgtttggc ttgctattta ccgaactcaa tagtgcccag
     1141 gcaaaaatca ttgcggaacg tatgcgtaaa aatgtcgaac tcctgaccgg ctttagtaac
     1201 agatatgatg ttcctgaaca aatgaccatc agtattggca cggttttttc aacgggtgac
     1261 acgcataata tctcgcttgt catgactgaa gcagataaag ccttacgcga agcgaaaagc
     1321 gaggggggca acaaagtgat tattcatcat atttaagtgc aaaaatattc agagccatgc
     1381 tttttgcgtg gcttttgcat acaatttatt actattaccc ctaaatttcc cctccccagc
     1441 ggcgcagaga tgagtataat tagcgcccct gtgccaggcc gcaatcgaac tttatctggt
     1501 tttctcgttt cactaaccga aggagtgcca tttatcatga aattgcacca tagaatgctc
     1561 cggcatttta tcgccgcaag tgtcattgtg ctgacatctt ccttccttat ttttgaactt
     1621 gtcgccagcg acagagcaat gagtgcctat ctgcgctata tcgtgcagag agcagactct
     1681 tcctttcttt atgataagta tcagaatcag agtattgccg cgcatgtgat gcgcgctcta
     1741 gctgctgagc agtcggaagt gccgccagaa cagcggcgcg ccatctgcga ggcttttgag
     1801 tctgccaata atacccatgg cttaaacctg actgcccata aatacccggg cttacgcggc
     1861 acactacaaa ccgcatccac tgactgcgac acaattgtgg aagctgcagc actattaccc
     1921 gcttttgatc aggcagtgga aggcaaccgc caccaggatg attacggttc aggtcttggg
     1981 atggccgaag agaaatttca ctattatctc gatctcaatg accgctatgt ctatttttat
     2041 gagccggtta atgttgaata ctttgcgatg aataactggt ccttcctgca gtcaggaagt
     2101 attggtatcg atcgcaaaga tattgaaaaa gtatttaccg ggcgtaccgt attatcgagc
     2161 atttaccagg atcagcgtac taaacagaac gtgatgagtt tgctgacgcc ggtatatgtc
     2221 gcagggcagc taaaagggat tgtgctgctg gatattaaca aaaacaatct gcggaatatc
     2281 ttttacactc atgaccgccc tctcctctgg cgttttctca atgtcacgct aaccgataca
     2341 gattcggggc gcgacattat catcaaccag agcgaagata atctgttcca gtatgtcagt
     2401 tacgtccatg acttaccggg cggcattcgt gtctcgttat ccattgatat tctttacttt
     2461 atcacgtctt cgtggaaaag cgttctgttc tggattttga cggcgttaat tttgctgaat
     2521 atggtgcgga tgcacttccg cttataccaa aatgtgtcgc gagaaaatat tagtgatgcg
     2581 atgactggac tctataatcg caaaatttta acccctgaac tggagcagcg gttgcagaaa
     2641 ctggtgcaat ccggttcttc ggtgatgttt attgctattg acatggacaa gttaaagcaa
     2701 ataaatgaca ccctcggtca tcaggagggg gatttagcga ttacgttatt agctcaggcg
     2761 attaaacaat cgattcgtaa aagtgattat gccatccgac tcggtggcga tgaattctgc
     2821 atcattcttg tcgattcgac gccgcaaatt gcagcacaac tgcctgaacg tatcgaaaaa
     2881 cgtctgcaac atatcgcgcc gcagaaagag atcggcttct cttccggtat ttacgcgatg
     2941 aaagaaaacg atacgttaca tgatgcgtat aaagcttccg atgagcgttt atatgtcaat
     3001 aagcagaaca aaaacagccg ttcatgataa ccttctgtgg ttgtttgctt gtaatctcag
     3061 gagcatgaaa tgactgaaat ggctaaagga agcgtgacgc atcagcggtt aatcgcttta
     3121 ttatcacaag aaggtgctaa cttccgcgtc gttacccatg aagcggtagg taaatgtgaa
     3181 gcggtgtcag aaattcgtgg caccgcttta ggccaggggg ctaaggcact ggtatgtaaa
     3241 gtcaaaggca atggcgtaaa tcaacatgtt ctggcgatcc tcgctgccga tcaacaggcc
     3301 gatctgagcc aactcgccag ccatataggc ggattacgcg cttcgttggc cagcccggcg
     3361 gaagtcgatg aactcacagg ctgtgtcttc ggcgcgatcc cccctttcag cttccatcca
     3421 aaactcaaac tggttgccga ccccctactc ttcgaacgat ttgatgaaat cgccttcaat
     3481 gcaggcatgc tggataaatc cgttattttg aaaaccgcgg attatttgcg tattgcgcaa
     3541 ccagaactgg tcaatttccg ccgcactgcg taactagcct gtccgttcaa taagcagaac
     3601 ggacaaaacc agcacagcag caatggcaaa aaacgatgac gtgataatca gtgtctcgac
     3661 gaacatttga tcgttcatag tatgcccccg ggagatagct gttttcacgt tattgttagc
     3721 gtgcacaaat ggcagtttga tgacagttcg cctgattttt tatgcagaaa acgtaaagat
     3781 tttctacttc ctgcctgcag caagcgtaaa gtaagcaggc ttattatttt ttggcaagga
     3841 aaccacgatg tttgatgtca ctttgctgat cctgctcggg ttagctgcgc tgggctttat
     3901 cagtcataac accactgtcg ccgtttcaat tctggtgttg atcattgtcc gcgtcacacc
     3961 gttaagcacc tttttcccgt ggattgaaaa acaggggcta agtatcggga taatcattct
     4021 gacgattggc gttatggctc ccatcgccag cgggacgcta ccgccctcaa ccctgattca
     4081 ctcgtttctg aactggaaat cgctggtggc aattgcagtc ggcgttattg tctcctggct
     4141 gggggggcgc ggcgtgacgt taatgggcag ccagccgcaa ctggtcgccg ggctgctggt
     4201 gggcactgtt ttaggcgtgg cgctgtttcg cggcgtaccg gttggcccgc ttattgccgc
     4261 gggtctggtt tcgctgattg tggggaaaca gtagttaatc ccgcaatata tcggcctggc
     4321 gtttgcccca ggcctttctt aaacatagta ataaaggccg ttgttgaatc ataccccagc
     4381 gtatgagcaa ctttctgtac agtatcgcct ttcactaatc cctgaagcgc cataatcagc
     4441 tgtaactgct gacgccactg gcgaaaactc aaccccgtct ctttcactat cagccgggca
     4501 agattgcgtt cactcatcgc aaaaaagcca gcccattgcc ccaatgagcc ccactcgacg
     4561 ggctctttcg ccatcatctc caccatcgtg cggattttag gatgagaaga aacgggcaag
     4621 tgcagctgtt gttgtggctg ctgagggagt tcatcaaaca ggacctgaat taatctgcgg
     4681 gtcatcggct cagcacgctg agtcgtcgtc ctgtttgcca gggttaaaat taattcccgg
     4741 cataagggcg atattttcaa tgtgcaacag gttgtcggca ttgtcactgc gctgggttcg
     4801 ataaacagaa aacagagttc agcgttagct gtcacttgat tgctatgttc cacccctccc
     4861 ggtatccaaa ccgcatattg tggcggcacc atccacaaag cattttccac cgtacaggta
     4921 attgcaccat gtagtgccag tatcaactgc cctttgcggt gctgatgtac aggactggtt
     4981 aactcatccg ttccggcgtg aatacaaaat gcgaccgcgg cttcatggtg tcggtcgggt
     5041 tcatagccat tgagattcaa cctgtgcatc attttgtccg aatttagcga tattttgtca
     5101 ttttagcttg attcaacata acaataaaaa cggtaaggta cagcctcgtt tgtaacaatg
     5161 agaagcatat gacctgttca acttcattaa gcggtaaaaa caggattttc cttatcgctg
     5221 gcattctgat gattgccaca acattacgcg tcacctttac cggcgcagca ccgttactgg
     5281 atacgattcg ttccgcttac tcgctaacga cggcgcaaac cggcttattg accaccctgc
     5341 cattattagc ctttgcgcta atctcaccgc tggccgcccc ggtggcgcga cgttttggta
     5401 tggaacgtag cctgtttgcc gcgttacttt tgatctgtgc aggtatcgca attcgctctc
     5461 tcccttcgcc ttacttatta tttggcggta cagcgatcat tggcggtggg attgcattag
     5521 gtaatgtctt actgccagga ttaattaaac gcgatttccc tcattccgtc gccagactta
     5581 ccggcgcata ttccctgaca atgggagctg cggcggctct gggatcggcg atggtagtgc
     5641 cgctggcttt gaacggtttt ggctggcaag gcgcgttgct catgctgatg tgttttcctc
     5701 tgctggctct ttttttatgg ctgccacagt ggcgaagcca acaacatgca aatttgagta
     5761 cttcacgcgc cttacatact cggggtatct ggcgttcgcc gcttgcctgg caggtaacat
     5821 tgtttcttgg gatcaactca ctggtctatt acgtgattat tggctggctt ccggcgatcc
     5881 tcatcagtca cggctatagc gaagcacagg cgggttcact gcatggtttg ctgcaactag
     5941 ccacagcagc tcccggtttg ctgatcccac ttttcttaca ccatgtgaaa gatcagcgtg
     6001 gtattgcagc gttcgttgcc ttgatgtgcg cagtgggcgc ggttgggctc tgctttatgc
     6061 cagcgcacgc gatcacctgg actctgcttt tcggctttgg ttccggcgca acaatgatac
     6121 tggggttgac gttcattggt ctgcgggcaa gttctgcgca tcaggcggcg gcactctcgg
     6181 ggatggcaca atccgtcggg tatttgctgg cagcctgtgg gccgccgctg atgggtaaaa
     6241 tacacgatgc taacggtgac tggtccgtac cactgatggg tgttgcaata ctttcactac
     6301 tgatggcgat tttcggactt tgcgccggga gagacaaaga aattcgctaa tatccggtgc
     6361 tatagtgacg tgacaaatca tgcgtgaaag ggagaacaaa cacgatgaat attcagtgca
     6421 aacgcgttta tgatccggct gaacagagcg atggttatcg cgtactggtc gaccgcctct
     6481 ggccgcgcgg tatcaaaaaa accgatttag cccttgatga gtgggataaa gaaatcacgc
     6541 cgtcaacgga actgcgcaaa gcctttcacg gcgaagtcgt cgattttgca acctttcgcg
     6601 agcaatatct tgcagaactg gcgcaacacg agcaagaagg aaagcggctg gcgaacatcg
     6661 ccaaaaaaca gccgctgacc ctgctctact cagcaaaaaa caccacgcag aaccatgcgc
     6721 tggtgctggc agactggcta cgtagcttgt gattttagta cgccatccgg cgattatttt
     6781 tcaccagccg gatggtcccg tcgccacaat gtccattcat caatcgtttc accgcccggt
     6841 aatttgcaat tgttgctgac cccttgcgct gtctgcacag gaatgagcgt cccgcccttc
     6901 tgctggcaat agaccgacgc cggatttgcc ataccaatct gcggcggttt aggtgcttct
     6961 ggctgagaag gggtggaaca accagccagc accagcaagc aaggcagaac aaaactgata
     7021 attttcattt attgatctca catatttatc cagacttaga gtatcgcggt atcgttttgt
     7081 tttgcagcac tatttttatt acattcactc aaaacatatt acgccttgtt tcatctttgt
     7141 tgatgatgtt ttatcatgcc tgcaaagatt aaataatcag catttacccg ccgtatcctg
     7201 gagttgtttc gtgtcagatc agattatcgc ccgcgtctcg caatcccttg ccaaagaaca
     7261 gtcactggaa agtctggttc gacagcttct ggagatgctg gaaatggtca ctgatatgga
     7321 gtcaacctac ctgaccaaag tggatgtcga agcgcgcctg cagcatatta tgtttgcccg
     7381 taacagccag aaaatgcaca tcccggagaa ttttaccgtc tcgtgggatt actcgttatg
     7441 caaacgcgcc attgatgaaa actgcttttt cagcgatgaa gtccccgacc gttggggcga
     7501 ctgtattgcg gcacgcaatc ttggcatcac cacatttctg agcacgccaa ttcacttacc
     7561 ggatggatca ttctatggca cgctttgcgc cgccagcagt gagaagcgcc agtggagtga
     7621 acgcgcggaa caggtcttac agttattcgc cggactgatt gcacaatata ttcaaaaaga
     7681 ggcactggtt gaacagctgc gcgaagctaa tgctgcgctg attgcgcaat cgtataccga
     7741 ctcgttaacc gggctaccga atcggcgggc gatttttgaa aatctgacga cgctgttttc
     7801 tctcgcccgg catcttaacc ataagataat gatcgcgttt atcgatctgg ataacttcaa
     7861 attaatcaat gatcgttttg gtcataatag tggcgatctg tttctcattc aggttggcga
     7921 gcgccttaat acgctccagc aaaatggcga agttattggt cgtctcggcg gtgatgagtt
     7981 tttggttgtt tcactgaaca acgagaatgc ggatatttcg tcgctgcgag aacgtattca
     8041 acaacaaata cgcggagaat atcacttagg tgatgttgat ttgtattatc ctggtgccag
     8101 tcttggcata gtagaagtcg atcctgaaac gaccgatgca gacagtgccc tgcatgctgc
     8161 cgatatcgcg atgtatcagg agaaaaaaca taaacagaaa acaccttttg tcacgcattc
     8221 agcgctacat tcctgaggcg tattcacatc cttttgattg gtgataacat gcgaatcggt
     8281 attatttttc cggttgtaat ctttattaca gcggtcgtat ttttagcatg gttttttatt
     8341 ggcggctatg ctgccccggg agcataaaga tgaaaaaaac aacgattatt atgatgggtg
     8401 tggcgattat tgtcgtactc ggcactgagc tgggatggtg gtaacgtcac atctaaaaaa
     8461 tagcaaaggc tgcctgtgtg cagcctttgt gcaatttacg cgttaacttt taatcttcct
     8521 gtagataaat agcacaacaa tcgcaccaat aacggcaacc acgaagctgc caaaattgaa
     8581 gccatcgact ttaccaaagc caaacagcgt gctgatccat ccgccaacta cggcaccgac
     8641 tatccccagt aggatagtca taaagaatcc accaccatct ttacctggca tgatccactt
     8701 cgccagaata ccggcaataa gcccaaaaat aatccatgac agaatgccca ttgtttcctc
     8761 acttatctgt tttgcattag cgggttagtc gctgataaaa agcatagcac aacatcggga
     8821 gggtaagatt tgtgacgagc atcacggagg aattttttgc gatggcgcag aaattgcgcc
     8881 atcaacgatc agtgataatt accaaccaca aacatcatgt tcgttttccg tgtcataaga
     8941 acgcacggta ttcaccagat cttttatcac ttcagccgcc acttctggca ccagcaaagt
     9001 catcggcgtc tctgtttcat aatcgacaga aacgccattg ctgttattgg tgacggtcac
     9061 ggtatacgtt gctttgccca tgattcattt cccgttatga atgactttcc gttgttgcgc
     9121 gccttccatc aggacttcag gagcaacgaa gaagtcaatg ttgaaataag tatcgtcagt
     9181 catggcttca atgttgcgcc acttttctgg agggaacacc gcaaactgcc ccgcttcgat
     9241 aaggatcacc tgatcaggct ctgcactgtg ttcatcagcg tagccgagat atttgaccgc
     9301 cccatgcata acggaaaggc gtgggtaaac ccccgggcgc gttcctttat caagatgacg
     9361 ttcgaatatt ccggcaggcg cagtttgttt attccagaaa ggcgttgagc gcgtatgaat
     9421 ataattctgt gggatttgca gcatccttta ccctccttcg gtgaatgcgc tgaaaacggt
     9481 ttattccagc cgtttcaggg tacgcctgat aatttgcatt ttaaatacca tttattggtt
     9541 acttttagca ccgtatcaga gaagaatcag ggaggattac aggtgggaag cccgcggcaa
     9601 cgcgggctga aagcatcagg attgcagcgt cgccagtcgg gcggcgaaac ccacgaacat
     9661 caaaccaatc agtgagttgc ccactttagc cagtttcttt ttggtacgta tgtactgcgt
     9721 gacaaaagcc ccagaaataa tcagaaagct caaatagcag aaactcacca gttccagcgt
     9781 cgtcgccaga ataaagaatg aaattcccgt atgtggggca ttaacatcga taaactgtac
     9841 gaaaaacgac acatagaaca aaatggcttt cggattagtc aggctcaaaa ttaacgcgcg
     9901 tttaaaaatg gcaccgtatt ggggctcatc ggatttggtc tcgctatttt tacctttcag
     9961 ggtcgcatag agaattttac tccccagata gagcaaataa aacgcaccaa gataacgtac
    10021 aatgttgaat aatatcgggg tggtcttaat taatgtcgcc actccagccc atgccagaaa
    10081 catcaatacc gcatcgccaa taaatacacc acaggccgca agataaccgc ctttcatacc
    10141 gctactgacg ctatttttga gtacaaacag ggtatttggc cctggcacca acacaataaa
    10201 aatggcccca accagatagg tccagtaatt cagaaccccg tattcagcga acacattaac
    10261 ctctttaatt atctttcgat catgcgcgat taaaggtgaa tatgctaacc aatctgtagc
    10321 ggcttagaaa ggagaaaatc aggttttaac tcgatatcaa cccgataatt gaatcattaa
    10381 tcaggcatgc tccagtgaaa aattcgggta gtgctcggca aaatactggc gtaaaaattc
    10441 taccgttatc cgcactttcg ctgacgtcgc cagccttgaa acataaacgg cccagacatt
    10501 cgctggctgg taatattccg gtagcacttg tactaaatga ccactggcgg gaaacagcct
    10561 ggcgacatcg atttttacgt ggtccggaaa aacaccgaag gcgaatattc ctcgccaggc
    10621 ggtagagtga atgaaggaac agagaatgaa gtcgtcattc aggaatcggt atttactcgt
    10681 cgtggtgtcg agcgcatttt gcgttaagct caaataagtt catttgaacc gcctgctgtt
    10741 ggatttgtag tttgccgaag ccggactgag cgcgcccagt ccggcttcgg aagattttac
    10801 tgtggatatt cctgcaataa attgtgtaat gcttccgcca tcagctcacc acgccagccg
    10861 gaaatcagct ccggcaaatt gttctgcggt ttcagtttcc agtgccagtt cagcagttgg
    10921 ttgatttgcc gacgcgatgc cagcaattcg gcgctgatct tgtgcgtttc gctgacgtca
    10981 gtaatcagcg acttaatcgc tttaaacgct ttacgataac ccggcatgtc catcaggtta
    11041 agcatcggct gcggtaaggc ctcttccggc aatgcctgcg ctttttccac cagcgccagc
    11101 aacgttttac cgtggaagcg gatttcgcta ccagataagc ccaggctgtc cagttcgccc
    11161 agacttcccg gcatataacg cgctaccgac cacaaatgct cttcacgcac gacaaagttc
    11221 accgccagat cgcgctctcg cgccttgcgc aatcgccagt cggctaacag ttgcagacag
    11281 gccagttggc gtgtgcgtaa ctgccaggca ttggtgatat cgcgccaggc gtcttccggc
    11341 gcaacgactt cctgacgacg catttgcatc aggcggcatt catccagcgc cgcaggtagc
    11401 cagccggagg cctccgtttc taccataagc tttgcggtga tcggtaacag ataccagaca
    11461 tccgccgctg catattcaca ctgacgttcg gtcagcggtc tggccagcca gtcggtgcgc
    11521 gattcactct tgtccagcgt aaggccggaa tactcttcca ccatggaagc gaaaccccat
    11581 gacatcgggc gtccgcagaa ggcagcaagg atttgcgtgt caatcaaggg ttgtggtaat
    11641 tcgccaaaga cattgaggaa cacttccaga tcttcactac ctgcatgaag aaattttgtg
    11701 atggacggat cgcgcaggat cgctttcagc ggtgaccagt cggtgatccc gagaggatca
    11761 attagcgcca gatgctcacc atcaaaaagt tgaattaaac ccagctgcgg gtaataagtg
    11821 cgcgtacgaa caaattcagt atccagggct atcgccggaa aggcacggac ggcttcacac
    11881 aaagaagcca gcgcatcgtc cgtggtaatc atttggtaat tcaaattgta ttctctttag
    11941 tgggcgtcaa aaaaaacgcc ggattaaccg gcgtctgacg actgacttaa cgctcaggct
    12001 ttattgtcca ctttgccgcg cgcttcgtca cgtaattctc gtcgcaaaat ttttccgacg
    12061 ttagatttcg gtaactcatc acgaaactcc accagcttcg gtactttgta gcccgtgagc
    12121 tgacggcggc aaaaagtcac cagtgactct tcggtaagcg atggatcttt tttcactacg
    12181 aagattttca ccgcttcacc actggagcca gaaggtacgc caacagccgc gacttcctgt
    12241 acgccaggat gctgcatgac gacatcttca atctcgttgg gatagacgtt aaaaccggaa
    12301 accagaatca tgtctttttt gcgatcgaca atgcgcagga atccttcttc atccatcacc
    12361 gcgatgtcgc cggtgtgtaa ccagccattt ttgatgattt catcggtagc atccggacgc
    12421 tgccagtaac ccagcatcac ctgcggtcct ttgacacaaa gctcacctgg ttgacccggt
    12481 ggtacttcat tatcatcatc atccaccagt ttggcttccg tcgacggcac cggtaaaccg
    12541 atgctaccac tatgataatc aatatcatac gggttaacgc tgaccagcgg cgcacactcg
    12601 gtcaggccat aaccttccag cagatactgt ccagtcagtt tcacccaacg ttctgccacc
    12661 acttgctgaa caggcatccc tccgcctgcg gaaagatgca gactggagaa atccagctgc
    12721 tggaactctt tattgttcag caacgcattg aacaaggtgt taacgccagt gatagcggta
    12781 aacggatatt tcgctaactc ttttaccaac cctggaatat cgcgcgggtt agtgataagc
    12841 aggttctgcc cacccagttc gataaacagc aggcagttaa tggtcagggc aaaaatgtga
    12901 tacagcggca gcgccgtcac caccagctct ttgcccggat gcaacagcgg accataggtc
    12961 gcgttaacct gttccaggtt cgccagcata ttgcggtgag tcagcatcgc gcctttcgcc
    13021 acaccagtgg tgccgccggt gtattgcagg aaagctaaat cttccggcac cagttctggt
    13081 ttgacatact gcatccggta gccgttgtgc agggcgctac gaaatgaaat ggcatctggc
    13141 agatggtatt tcggcaccag acgcttgatg tatttaacaa cgaaattgac taccgtgcct
    13201 tttgccgtag atagctgatc gcccatacgg gtcagaataa cgtgctgaac ggcggtttta
    13261 tcaaccactt tttccagagt gtgggcaaag ttagacacga taacaatcgc cgatgcgccg
    13321 ctatcgttaa gctgatgctc aagctcacgc ggggtataca acgggttaac gtttacgacg
    13381 atcatcccgg cacgcaaaat gccaaacagc gccaccggat attgcaataa gttaggcatc
    13441 atcaacgcaa cgcgatcgcc tttcttcagc cccaaccctt gttgcaaata agcggcaaac
    13501 gcgcgactac gttcttccag tttgcggaag gtcatcacct cccccatatt cacaaacgcg
    13561 ggttgatcgg cgtagcgcgc gaccgactgc tcaaacatat ctaccagaga ttgataacgg
    13621 tcagggttga tctccgtcgg aacgtccgcg ggataacggt taagccaaac cttcttcaat
    13681 tcttcacctc taaaatgcgt gttcgtcgtc atcgcaaccc caagcaataa acatatcgtt
    13741 aacataatat taactcatca taccagcttg ataattaccc aacgaaaagg ttgcgaagcg
    13801 cgtcactatt tatttttatc tttaccgtaa gaatgcagaa acagcggacc ggccgctgtt
    13861 tctttttctt taaaaacaag caattattca gttacaactg tttgtactct cgcggggccg
    13921 ggattatacc agccccatcc gccgtagcca tagggccagc cacgaccgcc ataaaaccat
    13981 ggatcaatcg gctgaggcgg cataatcacc tgctgggtta aatgccaacg tttgtaaccc
    14041 gttacttgca tcaccataaa tttatagggc gtattaccga ttttgccgtc aaccgcaccg
    14101 gtgattggcc cgactaccgt caccagttgt ccgcggaaat ccaccgggtc cagaaaaccg
    14161 ttcacatcgg cataaatgcg accgcgagaa ggttctccca gcgtcggtct ggctccgctg
    14221 tccagtggta cggtggcaat ttccaggcgg gttttccctt gctggttttg taccgcaacc
    14281 actttgccgc caaagcgtgc ctcctgacca acgtacagct gcggcgcact catcacccga
    14341 actaaatctt gttgcggcgt gggactgctg cctttaatgg cgtccggcac agtgacacaa
    14401 ccgctcagca ttagcgcaaa cgtacctgcc agtatgcctt tgataacatt cttttgaacc
    14461 gccatgatgc gactcctttt tttctcaggg catactctta agattcattc tttgcccgga
    14521 agtttcttcc atgcgacgtt gttacgtaaa taaactggtt cagcatgttc caccgccacc
    14581 gttttaccct cggcaaacat ctggcacgca atcggcagca tatcttctgc agcgggcagt
    14641 aacacttcgc catcgagcaa aaccagcccg ctctctttac cgagatccgg ccaggcttgc
    14701 cagcccgtcc ctactgtcac ccattcgccg gaaagctgtt gcattcgttc atgaacgagt
    14761 tcgggtttga gtacggcttc ggtttcttca ccgtgccaga taccgttttc atcacgctga
    14821 tattcggccc agtaaacttc gcccattcgc gcgtcaatag ctgccagcac gcgggttgcg
    14881 ccgtttttgc gccacgcccc ttgcgccatc gtcattagcg tggagacgcc aatcattggt
    14941 aactccgcgc caagtgccag tccttgcgca atgccaatac caatgcgcac gccggtgaag
    15001 ctaccggggc cgcgcccgta agccagagcg tttatgtcag tcagggaagt tccgctggtg
    15061 gtcaggatat cctgcaccat cggtaagatt cgttgagtat gttcacgagg gcaaagctca
    15121 aaatgagcgt tgacagtacc gtcgttccac agggcgacag agcaggcctc tgtcgcggta
    15181 tcgatagcca gaattcgcat gggtcttcgt gcttagatca ataaaaaggc gcgcatcata
    15241 ccatactccg taacaaatta cctggaggat ggtatcgcaa ggaaacgaac cgcacgggca
    15301 atgtcacggg tgcgcggcgc tggcggcaga ctggcgagaa acgtcgcgcc gtaagggcgc
    15361 atcaccagcc gattgtcgca aatcactaac acgccgcgat cgtcggcgtc gcgaatcaat
    15421 cgccctaccc cctgcttgag agtaatgacc gcatccggca gttgtacttc atcgaacgga
    15481 tcaccaccgc gcaaacggca atcttccatg cgtgctttta atagtggatc atccggcgag
    15541 gtaaacggca atttgtcgat aatcaccaat gacaatgtat cgccacgcac gtccacccct
    15601 tcccagaaac tgctggttgc cacaagaagc gcgttaccgg cgctgacaaa ttgctgcaac
    15661 agttgccctt tgctggtttc cccctgcaac aaaacgggaa gcgtcatggt agcgcggaac
    15721 tgctcggcca gatcgcgcat catggcgtgc gaggtacaaa gcataaaaca acgaccgttg
    15781 ttagcttcga tgatcggtcg cagcattgcc gccagttgcc gtgcggaacc cggttgattg
    15841 gtttgcggca ggttgcgcgg cacacagagt aacgcctggc ggctgtaatc aaaggggctg
    15901 ggtaacagca acgactctgc ctgttcaatg ccaagccgcg aggtgaaatg atgcagatcg
    15961 tcgtttaccg acagcgttgc tgaggtaaag atccagctac cggttttttg cgccattaac
    16021 tctttgaatt tatccgccac gctgagcggc gtgagagcca gagtaaaatg gcgcgaagtg
    16081 cattcgtacc agtagctgta gcccggctga ttgatctctt ttagccgttt cagccgtgtg
    16141 cgatataacg tggcgcgttc aaatgccgca tccagcaagg cggaacgccc cagcgacagt
    16201 ttcgccacgt cataacaaag ttccagagtg tcatcgagca gtaaaaatgc ccgctgaatt
    16261 tgcggattag ctaacagctc acgcaggtta ccgcgataac ctggctcacc gagttgcagg
    16321 cgaaaatcct gcgcactctg ggcaagacgg tccgcgcact tttgtaactg ctgggtgtct
    16381 tttaattcgg tgcgataggc gatggagatg tcttttgcca ggtcgagcag ttgtcgactg
    16441 gagagtgact gaccaaaata ctggctggca atgtcgggca gttggtgggc ttcgtcgaag
    16501 atcatgacgt cagcttccgg gatcagttcg ccaaatccac tctctttcac caccatatcc
    16561 gccagaaaga gatgatggtt taccaccacc acatcggcgt ccatcgcttt tttgcgtgct
    16621 ttgaccacaa agcaatcttt atacatcggg cagtcgctgc caaggcagtt atcgttggtg
    16681 ctggtgacca gcggccacgc ctgtgaatct tccgccacgc tgacgcaggt gctgatatca
    16741 ccatcgactg tttgattaga ccaggagcgc agcaggatca catcgcttaa gatttgtacc
    16801 ggcagatcgc cccccgccag cgcctgctgt tcgagacgtt cgaggcagag gtagtttgag
    16861 cgccctttca gcaacgccac gttgcccgtg tatttcaatg cctttgagac cgttggcaaa
    16921 tcgcggctgt agagctgatc ctgcaacgct tttgaaccgg tcgagataat gactttcttt
    16981 ttcgcccgca gcgcaggggc caggtaagcg taggttttgc ccgtaccggt tccggcttcc
    17041 accaccagcg gctggccttt ttctattgcc tgggtgacgg ctaccgccat ctgtcgctgt
    17101 ggttctcgcg gcttaaagcc tggtatcgct ttcgccagct gaccgtctgg tgcaaaatcg
    17161 tccgtcacac taccccctgt tgatttgaac aggaattatg tcaggatgag ggcgctttcg
    17221 ccagttgaag tggtgacggc gacctcacat tgtggcagtc tttgcagcac gaaaatggaa
    17281 ggataatgag gacaaaatga tgactatcgt tcgtatcgat gctgaagccc gctggtctga
    17341 tgtggtgatc cacaacaaca cgctctacta cactggtgta ccggaaaacc tcgacgccga
    17401 tgcttttgag caaaccgcca acacgctggc gcagattgac gccgtgctgg aaaaacaggg
    17461 cagcaataag tcgagcattc tggatgccac cattttcctg gctgataaaa acgacttcgc
    17521 ggcgatgaat aaagcgtggg atgcctgggt tgtcgcgggt catgcgccgg tgcgctgcac
    17581 ggtacaagcg ggtttgatga acccgaagta taaagttgaa attaagattg tggctgcggt
    17641 gtaagcttta tcgaagcaaa ataagtcaga cgataattta tcgataatac tggtcggttt
    17701 tgcataaatc gaccagagat taagttatta ctgttgatgc tcctgctaat tcattattta
    17761 tttttataat ttcaatttca tctataaaac tagctatata aataaacata accatgccgt
    17821 tgtgttcgcc ctctgattag caactctggt tttctatctt agctgaacaa tttggttata
    17881 gcgtgagaac tgaccgttaa ttttctccct cttactggat aacacaccat taggtgctac
    17941 ctttaaaaca cctgaatgtc aacaggttaa ctcgtgccat atcgttataa tccctttgat
    18001 attagatgca aattaaggtc atatatagcc ttatcgataa catggttaat tttaaggata
    18061 agaatatgcc tgctgtaata gataaagccc tggatttcat tggtgccatg gatgtatcag
    18121 caccaacacc aagttcgatg aatgaaagca cggcgaaggg aatctttaaa tatttgaaag
    18181 aactgggagt acccgcgagt gccgctgata ttacggcgcg agctgaccag gaaggctgga
    18241 acccggggtt cacggaaaaa atggttggat gggcaaaaaa aatggagtct ggtgaacgca
    18301 ttgtgattaa aaatcctgaa tacttttcaa catatatgca ggaagaacta aaagcactgg
    18361 tctaagttaa atttacatca gcataaatgg gtcaggacgc ttttaatcac atatgaaaag
    18421 cgtccttttt ctcacaatca aataatcatc ggcttctata acgaaagcga tacgggttag
    18481 tgtgttactc gtcttcatcc tcaaaacgcg ccacgattcg ctcgccggaa tggttggcac
    18541 gcagctcttc tgccaccaac gcgattgcct ggccgctgct catcccttga gccatcagtt
    18601 cctggatccg ctcgacagct ttttgctgct gttcatgggt gagtgaaggt aaacctgcaa
    18661 acattgttaa ctcctgctaa attgttggcg ctaattattt catgctaccc ggcacatagc
    18721 cagtagagtc aggactgatg aagacgttat ctcccgctgt gattacttta ccctggcgtc
    18781 aggacgccgc tgaattttat ttctcacgct taagccacct gccgtgggcg atgcttttac
    18841 actccggcta tgccgatcat ccgtatagcc gctttgatat tgtggtcgcc gagccgattt
    18901 gcactttaac cactttcggt aaagaaaccg tagtgtgtga aggagaaacc agttcaacaa
    18961 ctaccgacga tccgctacag gtgctccagc aggtgctgga tcgcgcagac attcacccag
    19021 cgcataacga agatttgcca tttcagggcg gcgcgctggg gttgtttggc tacgatctgg
    19081 gccgccgttt tgagtcactg ccagaaattg cggaacaaga tatcgttctg ccggatatgg
    19141 cagtgggtat ctacgactgg gcgctggttg tcgaccacca gcgtcaaaca gtttctttgc
    19201 tgagtcataa tgatgtcaat gcccgtaggg cctggctgga aaaccagcaa ttctccctgc
    19261 aggaagattt cgcgcttact tccgactggc aatccaatat gacccgcgag cagtacggcg
    19321 aaaaatttcg ccaggtacag gaatatctgc acagcggtga ttgctatcag gtgaatctcg
    19381 cccagcgttt tcatgcgacc tattctggcg atgaatggca ggcattcctt cagcttaatc
    19441 aggccaaccg cgcgccgttt agcgcttttt tacgccttga gcagggcgcg attttaagcc
    19501 tttcaccaga gcggtttatc ctttgtgata atagtgaaat ccagacccgc ccgattaaag
    19561 gcacgctacc acgcctgccc gatcctcagg aagatagcaa acaagcagag aaactggcga
    19621 actcagcgaa agatcgtgcc gaaaatctga tgattgtcga tttaatgcgt aatgatatcg
    19681 gtcgtgttgc cgtagcaggt tcggtaaaag taccagagct cttcgtggtg gaacccttcc
    19741 ctgccgtgca tcatctggtc agcaccataa cggcgcaact accagaacag ttacacgcca
    19801 gcgatctgtt tcgcgcggct tttcctggtg gctcaataac cggggcaccg aaagtacggg
    19861 ctatggaaat tatcgacgaa ctggaaccgc accgacgcaa tgcctggtgc ggcagcattg
    19921 gctatttgag cttttgcggc aacatggaca ccagcattac tatccgcacg ctgactgcca
    19981 ttaacggaca aatttactgc tctgcgggcg gtggaattgt cgccgatagc caggaagaag
    20041 cggaatatca ggaaactttt gataaagtta ataagatatt acgccaactg gagaagtaag
    20101 acgtggaata ccgtagcctg acgcttgatg attttttatc gcgctttcaa cttttgcgcc
    20161 cgcaaattaa ccgggaaacc ctaaatcatc gtcaggctgc cgtgttaatc cccatcgtcc
    20221 gtcgaccgca accggggttg ttgctgactc agcgttcgat tcatctgcgt aaacacgctg
    20281 gacaagtggc attccctgga ggcgcagtcg atgacacgga cgcatcagtt atcgccgctg
    20341 cgctgcgcga agctgaagaa gaggtcgcta taccgccttc cgccgttgag gttatcggcg
    20401 tgctgccgcc cgtcgatagc gtcactggct atcaggtaac cccagtggtc ggtattatcc
    20461 cgcccgatct gccgtatcgc gccagtgaag atgaagtctc cgccgtcttt gaaatgccgc
    20521 tcgcccaggc attacatctg ggtcgttatc atcctttaga tatctaccgc cgtggcgatt
    20581 cacatcgggt atggctgtcc tggtacgaac agtattttgt atggggaatg accgcaggca
    20641 taattcgtga gctggcgctg caaattggtg tgaaaccctg actatactta tctttacatc
    20701 tacaaaacac tacttgagac aatcatcgca atattagtta aatcgcggtt tttgattagt
    20761 ttaattcatg tgaatagtta agccagtcgc cgcgttccct cttacactat gcgctgttat
    20821 tagttcgtta ctggaagtcc agtcaccttg tcaggagtat tatcgtgatt agtctattcg
    20881 acatgtttaa ggtggggatt ggtccctcat cttcccatac cgtagggcct atgaaggcgg
    20941 gtaaacagtt cgtcgatgat ctggtcgaaa aaggcttact ggatagcgtt actcgcgttg
    21001 ccgtggacgt ttatggttca ctgtcgctga cgggtaaagg ccaccacacc gatatcgcca
    21061 ttattatggg tcttgcaggt aacgaacctg ccaccgtgga tatcgacagt attcccggtt
    21121 ttattcgcga cgtagaagag cgcgaacgtc tgctgctggc acagggacgg catgaagtgg
    21181 atttcccgcg cgacaacggg atgcgttttc ataacggtaa cctgccgctg catgaaaacg
    21241 gtatgcaaat ccacgcctat aacggcgatg aagtcgtcta cagcaaaact tattattcca
    21301 tcggcggcgg ttttatcgtc gatgaagaac actttggtca ggatgctgcc aacgaagtga
    21361 gcgtgccgta tccgttcaaa tctgccaccg aactgctcgc gtactgtaat gaaaccggct
    21421 tttcgctgtc tggtctcgct atgcaaaacg aactggcgct gcacagcaag aaagagatcg
    21481 acgagtattt cgcgcatgtc tggcaaacca tgcaggcatg tatcgatcgc gggatgaata
    21541 ccgaaggcgt actgccaggc ccgttgcgcg tgccacgtcg tgcgtcggcc ctgcgccgga
    21601 tgctggtttc cagcgataaa ctgtctaacg atccgatgaa tgtcattgac tgggtaaaca
    21661 tgtttgcgct ggcagttaac gaagaaaacg ccgccggtgg ccgtgtggta actgcgccaa
    21721 ctaacggtgc ctgcggtatc gttccggcag tgctggctta ctatgaccac tttattgaat
    21781 cagttagccc agacatctat acccgttact ttatggcagc gggcgcgatt ggtgcactgt
    21841 ataaaatgaa cgcgtctatt tccggtgcgg aagtcggttg ccagggcgaa gtaggtgttg
    21901 cctgttcaat ggctgctgcg ggccttgccg agctgttggg cggtagcccg gaacaggttt
    21961 gtgtggcagc ggaaattggc atggaacaca acctcggtct gacctgcgac cccgttgcag
    22021 ggcaagttca ggtgccgtgc attgaacgta atgccattgc ctctgtgaag gcgattaacg
    22081 ccgcacggat ggctctgcgc cgcaccagtg caccgcgcgt ctcgctggat aaggtcatcg
    22141 aaacgatgta cgaaaccggt aaggacatga acgctaaata ccgcgaaacc tcacgcggtg
    22201 gtctggcaat caaagtccag tgtgactaat acttcctact cgcccatctg caacggatgg
    22261 gcgaatttat tcccgcttcc tcgtctgctg taattttccc cactacactt ccactgttgc
    22321 gtcaggcgtt tgtcgccata cgcttacagg gtggcccgca tgcaaaaagc acaacggatc
    22381 attaaaacct atcgccgtaa tcgaatgatt gtttgtacga tttgcgcact cgttacgctc
    22441 gcttcgaccc tgagcgtgcg atttatttca cagcgtaact taaatcaaca acgggtagta
    22501 caattcgcca atcacgctgt agaggaatta gataaagtac tgcttcccct acaggcaggt
    22561 agcgaagtct tgcttccgct gattggtctg ccctgctctg tcgcccattt gccattacgt
    22621 aaacaggctg caaaactcca aactgtgcga tccattggcc tggtgcaaga cggcacactt
    22681 tattgctcca gcatttttgg ttatcgcaat gtgcccgtcg tggacattct ggctgaactt
    22741 cctgcaccgc aaccactttt acgactgacg accgaccgtg ccctgataaa aggcagtccg
    22801 gttttgattc aatggacgcc tgcagcgggc agtagcaatg ctggggtcat ggagatgatt
    22861 aacatcgact tactgacggc aatgctcctt gaaccacaac tgccgcaaat cagtagcgcc
    22921 agcctgacgg tggacagtcg gcatttgctc tatggtaatg ggctggtaga ttcccttccg
    22981 caacctgaaa acaatgaaaa ctatcaggtt tcttcgcaac gctttccttt taccattaac
    23041 gttaatggcc caggggctac ggcgctggca tggcactatc ttccaacaca attaccgctg
    23101 gcggtgctgc taagtttact ggtgggctac atcgcctggc tggcgaccgc ttaccgaatg
    23161 agcttttccc gcgagatcaa tctgggcctg gcgcaacatg agttcgaatt gttctgtcag
    23221 cctttgctta atgcgcgcag ccagcaatgt attggtgtag agattttgct gcgctggaac
    23281 aatccacgtc agggctggat ttcaccggat gtgtttattc ctatcgcgga agaacatcat
    23341 ttaattgtgc cactaacccg ctatgtgatg gccgaaacca ttcgtcagcg ccatgttttc
    23401 ccaatgagta gtcagtttca tgttggcatt aacgtcgcac ccagccattt tcgccgtggt
    23461 gtgctgataa aagatctcaa tcagtactgg tttagcgctc acccgattca gcaactgatc
    23521 ctcgaaatca ccgaacgcga tgccttactg gatgttgatt accggattgc ccgcgagctg
    23581 catcgtaaaa acgtcaaact ggcgattgat gacttcggca ccggcaacag ttcattttcc
    23641 tggcttgaaa cattacgtcc tgacgttctg aaaattgata agtcatttac cgcagctata
    23701 ggttctgatg cggttaactc gacggtgacc gatatcatca tcgcactggg gcaaagactg
    23761 aatattgaac tggtggcgga gggcgtagaa acgcaagaac aggcgaagta tttgcgcagt
    23821 catggcgtgc atattttgca agggtatttg tacgcacagc cgatgccgct acgtgatttt
    23881 cccaaatggc tggcgggcag ccaaccgccg cccgcccggc ataatggaca tatcacaccc
    23941 attatgccgt tacgttaacg cttactcatc ttcatcgtgc gccggttgct ctttaacaat
    24001 gcgaaccaga tcaacacgat aatcattggc ttcaatgatg gtgatatgca gtggccctac
    24061 atcaatcaca tcgcccacac ggggaatgtg accatttgcc gagatcacga ggcccgcgac
    24121 cgtcgcgata tcatcgtcat cggcaaggtg ctcaacatca agcgcctgct gcaaggcatg
    24181 caaatctgta ccgcctttta ccagccagcc gtcaccgtcg gtaatgattt ccggcgtttc
    24241 gtcagcgtcc gggaattcac ccgcaatggc ttccagcaca tccagcggcg tgaccagacc
    24301 ttgtaccaca ccaaactcgt tggtcacgat aacaaagctc ccgcgagcac gacgcagcac
    24361 gcccagcagg ttgatcggat cgagggtttc cgggacgata atcgccggag acgccgaagc
    24421 aatcgccgcc acatcaacgc cctcttccag cgccaccagc agttctttag cacgtacgat
    24481 gccgataatt tcatccagtt caccgcgaca taccgggaac aaactgtgcg gtgaggagag
    24541 cagctgctcg cggatttcat cgaccccgag attagcatca acccagctta tttcaccgcg
    24601 cggcgtcatg atcccgcgca gagaacgcga cgccagcgtc agtacgccgt taatcatgta
    24661 acgttcttct tcggcaaacg caccttccgg gatcggcatc ggcatcgggt tatcggcatc
    24721 gtgctgaaca ttggcctgac gtttcccgcc catcaaacgc aggatggcat cggcagtacg
    24781 cgctcgcagc ggcaaagtcg actggtgacg aataaagttg cgacgcgcaa tctggttaaa
    24841 cacttcgatg atgatcgaga agccaatcgc ggcatacagg taacctttcg gaatgtggaa
    24901 accgaaacct tctgccacca gactcagacc aatcattaac aggaagctca gacagagcac
    24961 caccaccgtg gggtgctggt taacgaatcg cgtcagcggt tttgatgcca gcagcataac
    25021 tgccatcgca atcactaccg ccgccatcat caccggcaaa tggttaacca tccctactgc
    25081 agtaattacc gcatccaacg agaagacggc gtcaaggatg acaatctgtg tgacgaccac
    25141 ccagaaactg gcgtagcctt taccgtggcc ggaatcatga tcgcggtttt ccagccgttc
    25201 atgcagttcg gttgttgctt tgaacagcaa gaatatgccc ccgaacaaca taatcaggtc
    25261 gcgtccggag aaggagaaat ccatgacggt aaatagcggt ttggtcagcg tgaccatcca
    25321 tgaaatcagc gacagcagcc ccagacgcat aatcagcgcc agtgataatc ccagcaaacg
    25381 cgctttatcg cgttgttttg gcggcagttt gtcagcaaga atggcgatga agaccaggtt
    25441 atcgataccc agcacaattt cgagaacaac aagcgtgagt agccccgccc aaatcgaggg
    25501 gtccattaag aattccatga caagctcctg cttaaggaat agctattcga cgccagaaat
    25561 aatgcaggcg taacgacaaa atgcaaacga aaggtgcggc ataaagtgcc agaaaggcag
    25621 gcgttaaaag gcctgatgct gaaatgacgt cggtgacgat ccatactgcg ggctactgcc
    25681 ctatactcca tggttgttaa acaggagtta aacatatcag agacgcctct gatttggcaa
    25741 agatttacct tcctttgcaa acgaatgtga caaggatatt ttacctttcg aaatttctgc
    25801 taatcgaaag ttaaattacg gatcttcatc acataaaata attttttcga tatctaaaat
    25861 aaatcgcgaa acgcaggggg tttttggttg tagcccttat ctgaatcgat tcgattgtgg
    25921 acgacgattc aaaaacacat ctggcacgtt gaggtgttaa cgataataaa ggaggtagca
    25981 agtgaccatt gctattgtta taggcacaca tggttgggct gcagagcagt tgcttaaaac
    26041 ggcagaaatg ctgttaggcg agcaggaaaa cgtcggctgg atcgatttcg ttccaggtga
    26101 aaatgccgaa acgctgattg aaaagtacaa cgctcagttg gcaaaactcg acaccactaa
    26161 aggcgtgctg tttctcgttg atacatgggg aggcagccca ttcaatgctg ccagccgcat
    26221 tgtcgtcgac aaagagcatt atgaagtcat tgcaggcgtt aacattccaa tgctcgtgga
    26281 aacgttaatg gcccgagatg atgaccctag ctttgatgaa ctggtggcac tggcagtaga
    26341 aacaggccgt gaaggcgtga aagcactgaa agccaaaccg attgaaaaag ccgcgccagc
    26401 acccgctgcc gcagcaccca aagtggctcc aactccggca aaaccaatgg gaccgaacga
    26461 ctacatggtt attggccttg cgcgtatcga cgaccgtctg attcacggtc aggtcgccac
    26521 ccgctggacc aaagaaacca atgtctcccg tattattgtt gttagtgatg aagtggctgc
    26581 ggataccgtt cgtaagacac tgctcaccca ggttgcacct ccgggcgtaa cagcacacgt
    26641 agttgatgtt gccaaaatga ttcgcgtcta caacaacccg aaatatgctg gcgaacgcgt
    26701 aatgctgtta tttaccaacc caacagatgt agagcgtctc gttgaaggcg gcgtgaaaat
    26761 cacctctgtt aacgtaggtg gtatggcatt ccgtcagggt aaaacccagg tgaataacgc
    26821 ggtttcggtt gatgaaaaag atatcgaggc gttcaagaaa ctgaatgcgc gcggtattga
    26881 gctggaagtc cgtaaggttt ccaccgatcc gaaactgaaa atgatggatc tgatcagcaa
    26941 aatcgataag taacgtattg tgttgattat cattcagttt tcacacttta gtcttacgta
    27001 aacaggagaa gtacaatgga gattaccact cttcaaattg tgctggtatt tatcgtagcc
    27061 tgtatcgcag gtatgggatc aatcctcgat gaatttcagt ttcaccgtcc gctaatcgcg
    27121 tgtaccctgg tgggtatcgt tcttggggat atgaaaaccg gtattattat cggtggtacg
    27181 ctggaaatga tcgcgctggg ctggatgaac atcggtgctg ccgttgcgcc tgacgccgct
    27241 ctggcttcca tcatttctac tattctggtt atcgcaggtc atcagagcat tggtgcaggt
    27301 atcgcactgg caatccctct ggccgctgca ggccaggtac tgaccatcat cgttcgtact
    27361 attaccgttg ctttccagca cgctgcggat aaggctgctg ataacggcaa cctgacagca
    27421 atttcatgga tccacgtttc ttctctgttc ctgcaagcaa tgcgtgtggc tattccggcc
    27481 gtcatcgttg cgctgtctgt tggtaccagc gaagtacaga acatgctgaa tgcgattccg
    27541 gaagtggtga ccaatggtct gaatatcgcc ggtggcatga tcgtggtggt tggttatgcg
    27601 atggttatca acatgatgcg tgctggctac ctgatgccgt tcttctacct cggcttcgta
    27661 accgcagcat tcaccaactt taacctggtt gctctgggtg tgattggtac tgttatggca
    27721 gtgctctaca tccaacttag cccgaaatac aaccgcgtag ccggtgcgcc tgctcaggct
    27781 gctggtaaca acgatctcga taacgaactg gactaacagg tgagcgaaat ggttgataca
    27841 actcaaacta ccaccgagaa aaaactcact caaagtgata ttcgtggcgt cttcctgcgt
    27901 tctaacctct ttcagggttc atggaacttc gaacgtatgc aggcactggg tttctgcttc
    27961 tctatggtac cggcaattcg tcgcctctac cctgagaaca acgaagctcg taaacaagct
    28021 attcgccgtc acctggagtt ctttaacacc cagccattcg tggctgcgcc gattctcggc
    28081 gtaaccctgg cactggaaga acagcgtgct aatggcgcag agatcgatga cggtgctatc
    28141 aacggcatca aagtcggttt gatggggccg ctggctggtg taggcgaccc gatcttctgg
    28201 ggaacggtac gtccggtatt tgcagcgctg ggggctggta tcgcgatgag cggcagcctg
    28261 ttaggtccgc tgctgttctt catcctgttt aacctggtgc gtctggcaac ccgttactac
    28321 ggcgtagcgt atggttactc caaaggtatc gatatcgtta aagatatggg tggtggcttc
    28381 ctgcaaaaac tgacggaagg ggcgtctatc ctcggcctgt ttgtcatggg ggcattggtt
    28441 aataagtgga cacatgtcaa catcccgctg gtggtctctc gcattactga ccagacgggc
    28501 aaagaacacg ttaccactgt ccagactatt ctggaccagt taatgccagg cctggtacca
    28561 ctgctgctga cctttgcttg tatgtggctg ctgcgcaaaa aagttaaccc gctgtggatc
    28621 atcgttggct tcttcgtcat cggtatcgct ggttacgctt gcggcctgct gggactgtaa
    28681 gactgttgta cactaccggg gccttttggc cccgtttttt tatctggagg attaatgaca
    28741 atcacggacc tggtactgat tcttttcatc gccgcacttc tggccttcgc gatctacgat
    28801 cagttcatca tgccccgccg taacggcccc accctgctgg caattccttt gcttcggcgt
    28861 ggtcgcatcg atagcgttat cttcgtcgga ttgattgtca ttcttatcta taacaacgtc
    28921 acgaatcatg gcgcgcttat aacaacatgg ttattaagtg cactggctct gatgggtttt
    28981 tatatattct ggattcgcgt tccgaagatc atctttaaac aaaaaggttt tttcttcgcc
    29041 aatgtctgga ttgaatatag ccgaatcaaa gcgatgaact tgtcggaaga tggcgtgttg
    29101 gtgatgcaat tagaacagcg tcggctgtta atccgcgttc gaaatatcga cgatttggaa
    29161 aaaatttata agcttctcgt ttcaactcaa taagttatga atttagccaa agct
//
LOCUS       DADSYM010000049        29093 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2415
            SAMN08039579-rid11723173.denovo.049, whole genome shotgun sequence.
ACCESSION   DADSYM010000049 DADSYM010000000
VERSION     DADSYM010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039579
            Sequence Read Archive: SRR6362533
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 29093)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29093)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980162.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 03:39:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,484
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 4,347
            CDSs (with protein)               :: 4,347
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 43 of 85
            Pseudo Genes (incomplete)         :: 37 of 85
            Pseudo Genes (internal stop)      :: 19 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29093
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039579-rid11723173.denovo.049"
                     /strain="LGE2415"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8302 N 95.9628 W"
                     /collection_date="2015-08-24"
     gene            106..1689
                     /locus_tag="J1Y83_001822"
     CDS             106..1689
                     /locus_tag="J1Y83_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HBA7629972.1"
                     /translation="MEWIADPSIWAGLITLIVIELVLGIDNLVFIAILAEKLPPKQRD
                     RARVTGLLLAMLMRLLLLASISWLVTLTQPLFSFRSFTFSARDLIMLFGGFFLLFKAT
                     MELNERLEGKDSNNPTQRKGAKFWGVVAQIVVLDAIFSLDSVITAVGMVDHLLVMMAA
                     VVIAISLMLMASKPLTQFVNSHPTIVILCLSFLLMIGFSLVAEGFGFVIPKGYLYAAI
                     GFSVMIEALNQLAIFNRRRFLSANQTLRQRTTEAVMRLLSGQKEDAELDAETASMLVD
                     HGNQQIFNPQERRMIERVLNLNQRTVSSIMTSRHDIEHIDLNAPEDEIRQLLERNQHT
                     RLVVTDGDDAEDLLGVVHVIDLLQQLLRGEPLNLRVLIRQPLVFPETLPLLPALEQFR
                     NARTHFAFVVDEFGSVEGIVTLSDVTETIAGNLPNEVEEIDARHDIQKNADGSWTANG
                     HMPLEDLVQYVPLPLDEKREYHTIAGLLMEYLQRIPKPGEEVQVGNYLLKTLQVESHR
                     VQKVQIIPLRKDGEMEYEV"
     gene            complement(1757..3610)
                     /gene="asmA"
                     /locus_tag="J1Y83_001823"
     CDS             complement(1757..3610)
                     /gene="asmA"
                     /locus_tag="J1Y83_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane assembly protein AsmA"
                     /protein_id="HBA7629973.1"
                     /translation="MRRFLTTLMILLVVLVAGLSALVLLVNPNDFRDYMVKQVAARSG
                     YQLQLDGPLRWHVWPQLSILSGRMSLTAQGASQPLVRADNMRLDVALLPLLSHQLSVK
                     QVMLKGAVIQLTPQTEAVRSEDAPVAPRDNTLPDLSDDRGWSFDISSLKVADSVLVFQ
                     HEDDEQVTIRNIRLQMEQDPQHRGSFEFSGRVNRDQRDLTISLNGTVDASDYPHDLMA
                     AIEQINWQLQGADLPKQGIQGQGSFQVQWQESHKRLSFNQISLTANDSTLSGQAQVTL
                     TEKPEWQLRLQFPQLNLDNLIPLNETANGENGAAQQGQSQSTLPRPVISSRIDEPAYQ
                     GLQGFTADILLQASNVRWRGMNFTDVATQMTNKSGLLEITQLQGKLNGGQVSLPGTLD
                     ATSINPRINFQPRLENVEIGTILKAFNYPISLTGKMSLAGDFSGADIDADAFRHNWQG
                     QAHVEMTDTRMEGMNFQQMIQQAVERNGGDVKAAENFDNVTRLDRFTTDLTLKDGVVT
                     LNDMQGQSPVLVLTGEGMLNLADQTCDTQFDIRVVGGWNGESKLIDFLKETPVPLRVY
                     GNWQQLNYSLQVDQLLRKHLQDEAKRRLNDWAERNKDSRNGKDVKKLLEKM"
     gene            complement(3632..4213)
                     /gene="dcd"
                     /locus_tag="J1Y83_001824"
     CDS             complement(3632..4213)
                     /gene="dcd"
                     /locus_tag="J1Y83_001824"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HBA7629974.1"
                     /translation="MRLCDRDIEAWLDEGRLSINPRPPVERINGATVDVRLGNKFRTF
                     RGHTAAFIDLSGPKDEVSAALDRVMSDEIVLNESEAFYLHPGELALAVTLESVTLPAD
                     LVGWLDGRSSLARLGLMVHVTAHRIDPGWSGCIVLEFYNSGKLPLALRPGMLIGALSF
                     EPLSGPAARPYNRREDAKYRNQQGAVASRIDKD"
     gene            complement(4305..4946)
                     /gene="udk"
                     /locus_tag="J1Y83_001825"
     CDS             complement(4305..4946)
                     /gene="udk"
                     /locus_tag="J1Y83_001825"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HBA7629975.1"
                     /translation="MTDQSHQCVIIGIAGASASGKSLIASTLYRELREQVGDEHIGVI
                     PEDCYYKDQSHLSMEERVKTNYDHPSAMDHSLLLEHLQALKRGSAIDLPVYSYVEHTR
                     MKETVTVEPKKVIILEGILLLTDARLRDELNFSIFVDTPLDICLMRRIKRDVNERGRS
                     MDSVMAQYQKTVRPMFLQFIEPSKQYADIIVPRGGKNRIAIDILKAKISQFFE"
     gene            5264..8581
                     /locus_tag="J1Y83_001826"
     CDS             5264..8581
                     /locus_tag="J1Y83_001826"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HBA7629976.1"
                     /translation="MSKQSQHVLIALPHPLLHLVSLGLVSFIFTLFSLELSQFGTQLA
                     PLWFPTSIMMVAFYRHAGRMWPGIALSCSLGNIAASILLFSTSSLNMTWTTINIVEAV
                     VGAVLLRKLLPWYNPLQNLADWLRLALGSAIIPPLLGGVLVVLLTPGDDPLRAFLIWV
                     LSESIGALALVPLGLLFKPHYLLRHRNPRLLFESLLTLAITLTLSWLSMLYLPWPFTF
                     IIVLLMWSAVRLPRMEAFLIFLTTVMMVSLMMAADPSLLATPRTYLMSHMPWLPFLLI
                     LLPANIMTMVMYAFRAERKHISESETRFRNAMEYSAIGMALVGTEGQWLQSNKALCQF
                     LGYSQEELRGLTFQQLTWPEDLNKDLQQVEKLISGEINTYSMEKRYYNRNGDVVWALL
                     AVSLVRHMDGTPLYFIAQIEDINELKRTEQVNQQLMERITLANEAGGIGIWEWELKPN
                     IFSWDKRMFELYEIPPHIKPNWQVWYECVLPEDRQHAEKVIRDSLQSRSPFKLEFRIA
                     VKDGIRHIRALANRVLNKEGEVERLLGINMDMTEVKQLNEALFQEKERLHITLDSIGE
                     AVVCIDMAMKITFMNPVAEKMSGWTQEEALGVPLLTVLHITFGDNGPLMENIYSADTS
                     RSAIEQDVVLHCRSGGSYDVHYSITPLSTLDGSNIGSVLVIQDVTESRKMLRQLSYSA
                     SHDALTHLANRASFEKQLRILLQTVNSTHQRHALVFIDLDRFKAVNDSAGHAAGDALL
                     RELASLMLSMLRSSDVLARLGGDEFGLLLPDCNVESARFIATRIISAVNDYHFIWEGR
                     VHRVGASAGITLIDDNNHQAAEVMSQADIACYASKNGGRGRVTIYEPQQAAAHSERAA
                     ISLDEQWRMIKENQLMMIAHGIASPRLPEASNLWLISLKLWSCESEIIDEQTFRRSFS
                     DPALSHALDQRVCHDFFQQAAKAVASKGLSIALPLSVAGLSSATLVNELLEQLENSPL
                     PARLLHLIIPAEAIFDHAESVQKLRLAGCRIVFSQVGRDLQIFNSLKANMADYLLLDG
                     ELCANVQGNLMDEMLITIIQGHAQRLGMKTIAGPVVLPLVMDTLSGIGVDLIYGDVIA
                     NAQPLDLLVNSSYFAIN"
     gene            complement(8619..9476)
                     /gene="alkA"
                     /locus_tag="J1Y83_001827"
     CDS             complement(8619..9476)
                     /gene="alkA"
                     /locus_tag="J1Y83_001827"
                     /EC_number="3.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000288352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase 2"
                     /protein_id="HBA7629977.1"
                     /translation="MYTLNWQPPYDWSWMLGFLAARAVSGVETVADSYYARSLAVGEY
                     RGVVTAIPDIARHTLHINLSAGLEPVAAECLAKMSRLFDLQCNPQIVNGALGKLGAAR
                     PGLRLPGCVDAVEQGVRAILGQLVSVAMAAKLTSRVAQLYGERLDDFPDYACFPTPQR
                     LAVADPQALKALGMPLKRAEALIHLANAALEGTLPMTIPGDVEQAMKTLQTFPGIGRW
                     TANYFALRGWQAKDVFLPDDYLIKHRFPGMTPAQIRRYAERWKPWRSYALLHIWYTEG
                     WQPDEADEL"
     gene            9610..10962
                     /gene="yegD"
                     /locus_tag="J1Y83_001828"
     CDS             9610..10962
                     /gene="yegD"
                     /locus_tag="J1Y83_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HBA7629978.1"
                     /translation="MFIGFDYGTANCSVAVMRDGKPHLLKMENDSTLLPSMLCAPTRE
                     AVSEWLYRHHDVPADDDETQALLRRAIRYNREEDIDVTAKSVQFGLSSLAQYIDDPEE
                     VWFVKSPKSFLGASGLKPQQVALFEDLVCAMMLHIRQQAQAQLPEAITQAVIGRPINF
                     QGLGGDEANTQAQGILERAAKRAGFKDVVFQYEPVAAGLDYEATLQEEKRVLVVDIGG
                     GTTDCSLLLMGPQWRARLDREASLLGHSGCRIGGNDLDIALAFKNLMPLLGMGGETEK
                     GIALPILPWWNAVAINDVPAQSDFYSSANGRLLNDLVRDAREPEKVALLQKVWRQRLS
                     YRLVRSAEESKIALSSVAETRASLPFISDELATLISQQGLESALSQPLARILEQVQLA
                     LDNAQEKPDVIYLTGGSARSPLIKKALAEQLPGIPIAGGDDFGSVTAGLARWAEVVFR
                     "
     gene            complement(10975..12915)
                     /gene="yegI"
                     /locus_tag="J1Y83_001829"
     CDS             complement(10975..12915)
                     /gene="yegI"
                     /locus_tag="J1Y83_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310906.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase YegI"
                     /protein_id="HBA7629979.1"
                     /translation="MKPTLYTATGECVTPGRELGKGGEGAVYDIEEFVDSVAKIYHTP
                     PPALKQDKLAFMAATADAQLLNYVAWPQATLHGGRGGKVIGFMMPKVSGKEPIHMIYS
                     PAHRRQSYPHCAWDFLLYVARNIASSFATVHEHGHVVGDVNQNSFMVGRDSKVVLIDS
                     DSFQINANGTLHLCEVGVSHFTPPELQTMPSFVGFERTENHDNFGLALLIFHILFGGR
                     HPYSGVPLISDAGNALETDIAHFRYAYASDNQRRGLKPPPRSIPLSMLPGDVEAMFQQ
                     AFTESGVATGRPTAKAWVAALDLLRQQLKKCTVSAMHVYPAHLTDCPWCALDNQGVIY
                     FIDLGEEVITTGGDFVLARVWAMVMASVAPPALQLPLPDHFQPTGRPLPSGLLRREYI
                     ILIEIALSALSLLLCGLQAEPRYIILVPVLAAIWIIGSLTSKAYKAEIQQRREAFNRA
                     KMDYDHLVSQIQQLGGLEGFIAKRARLEKMKDEILGLPEEEKRALVALHDTARERQKQ
                     KFLEGFFIDVASIPGVGPARKAALRSFGIETAADVTRRSVKQVKGFGDHLTQAVIDWK
                     ASCERRFVFRPNEAVTPADRQAVMAKIAAKRHRLESALTVGATELQRFRLHAPARTIP
                     LMEPLRQAAEKQAQAQADLSRC"
     gene            complement(12912..13673)
                     /gene="pphC"
                     /locus_tag="J1Y83_001830"
     CDS             complement(12912..13673)
                     /gene="pphC"
                     /locus_tag="J1Y83_001830"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-serine/threonine phosphatase PphC"
                     /protein_id="HBA7629980.1"
                     /translation="MSWRLVYASAVGTSHISADLPCQDACQMQVAWLNDQQPLLSVFV
                     ADGAGSVSQGGEGAMLAVNEAMAYMSQKVQGGELGLNDVLATDIVLTIRQRLFAEAEA
                     KELAVRDFACTFLGLISSANGTLIMQIGDGGVVVDLGHGLQLPLTPMVGEYANMTHFI
                     TDEDAVSRLETFTSTERAHKVAAFTDGIQRLALNMLDNSPHVPFFTPFFNGLASATQE
                     QLDLLPELLKQFLSSPAVNERTDDDKTLALAMWLP"
     gene            complement(13670..14329)
                     /locus_tag="J1Y83_001831"
     CDS             complement(13670..14329)
                     /locus_tag="J1Y83_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707965.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HBA7629981.1"
                     /translation="MSEQITFATSDFASNPEPRCPCILLLDVSGSMSGRPINELNTGL
                     VTFRDELLADSLALKRVELGIVTFGPVHVEQPFTSAANFFPPILFAQGDTPMGAAITK
                     SLDMVEERKREYRANGISYYRPWIFLITDGAPTDEWQAAANKVFQGEEDKKFAFFTIG
                     VQGADMKTLAQISVRQPLSLQGLQFRELFSWLSSSLRSVSRSTPGTEVVLEAPKGWTS
                     V"
     gene            complement(14547..14603)
                     /locus_tag="J1Y83_001832"
     CDS             complement(14547..14603)
                     /locus_tag="J1Y83_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HBA7629982.1"
                     /translation="MMKLLIIVVLLVISWPAY"
     gene            complement(14875..14931)
                     /locus_tag="J1Y83_001833"
     CDS             complement(14875..14931)
                     /locus_tag="J1Y83_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HBA7629983.1"
                     /translation="MMKILIIVVLLVISYPAY"
     gene            15210..16457
                     /gene="mdtA"
                     /locus_tag="J1Y83_001834"
     CDS             15210..16457
                     /gene="mdtA"
                     /locus_tag="J1Y83_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter subunit MdtA"
                     /protein_id="HBA7629984.1"
                     /translation="MKGSYKSRWVIVIVVVIAAIAAFWFWQGRNDSQSATPGATKQAQ
                     QSPAGGRRGMRAGPLAPVQAATAVEQAVPRYLTGLGTITAANTVTVRSRVDGQLMALH
                     FQEGQQVKAGDLLAEIDPSQFKVALAQAQGQLAKDKATLANARRDLARYQQLAKTNLV
                     SRQELDAQQALVSETEGTIKADEASVASAQLQLDWSRITAPVDGRVGLKQVDVGNQIS
                     SGDTTGIVVITQTHPIDLLFTLPESDIATVVQAQKAGKPLVVEAWDRTNSKKLSEGTL
                     LSLDNQIDATTGTIKVKARFNNQDDALFPNQFVNARMLVDTEQNAVVIPTAALQMGNE
                     GHFVWVLNSENKVSKHLVTPGIQDSQKVVIRAGISAGDRVVTDGIDRLTEGAKVEVVE
                     AQSATAPEEKATSREYAKKGARS"
     gene            16457..19579
                     /gene="mdtB"
                     /locus_tag="J1Y83_001835"
     CDS             16457..19579
                     /gene="mdtB"
                     /locus_tag="J1Y83_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit MdtB"
                     /protein_id="HBA7629985.1"
                     /translation="MQVLPPSSTGGPSRLFIMRPVATTLLMVAILLAGIIGYRALPVS
                     ALPEVDYPTIQVVTLYPGASPDVMTSAVTAPLERQFGQMSGLKQMSSQSSGGASVITL
                     QFQLTLPLDVAEQEVQAAINAATNLLPSDLPNPPVYSKVNPADPPIMTLAVTSTAMPM
                     TQVEDMVETRVAQKISQISGVGLVTLSGGQRPAVRVKLNAQAIAALGLTSETVRTAIT
                     GANVNSAKGSLDGPSRAVTLSANDQMQSAEEYRQLIIAYQNGAPIRLGDVATVEQGAE
                     NSWLGAWANKEQAIVMNVQRQPGANIISTADSIRQMLPQLTESLPKSVKVTVLSDRTT
                     NIRASVDDTQFELMMAIALVVMIIYLFLRNIPATIIPGVAVPLSLIGTFAVMVFLDFS
                     INNLTLMALTIATGFVVDDAIVVIENISRYIEKGEKPLAAALKGAGEIGFTIISLTFS
                     LIAVLIPLLFMGDIVGRLFREFAITLAVAILISAVVSLTLTPMMCARMLSQESLRKQN
                     RFSRASEKMFDRIIAAYGRGLAKVLNHPWLTLSVALSTLLLSVLLWVFIPKGFFPVQD
                     NGIIQGTLQAPQSSSFANMAQRQRQVADVILQDPAVQSLTSFVGVDGTNPSLNSARLQ
                     INLKPLDERDDRVQKVIARLQTAVDKVPGVDLFLQPTQDLTIDTQVSRTQYQFTLQAT
                     SLDALSTWVPQLMEKLQQLPQLSDVSSDWQDKGLVAYVNVDRDSASRLGISMADVDNA
                     LYNAFGQRLISTIYTQANQYRVVLEHNTENTPGLAALDTIRLTSSDGGVVPLSSIAKI
                     EQRFAPLSINHLDQFPVTTISFNVPDNYSLGDAVQAIMDTEKTLNLPVDITTQFQGST
                     LAFQSALGSTVWLIVAAVVAMYIVLGILYESFIHPITILSTLPTAGVGALLALMIAGS
                     ELDVIAIIGIILLIGIVKKNAIMMIDFALAAEREQGMSPREAIYQACLLRFRPILMTT
                     LAALLGALPLMLSTGVGAELRRPLGIGMVGGLIVSQVLTLFTTPVIYLLFDRLALWTK
                     SRFARHEEEA"
     gene            19580..22657
                     /gene="mdtC"
                     /locus_tag="J1Y83_001836"
     CDS             19580..22657
                     /gene="mdtC"
                     /locus_tag="J1Y83_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit MdtC"
                     /protein_id="HBA7629986.1"
                     /translation="MKFFALFIYRPVATILLSVAITLCGILGFRMLPVAPLPQVDFPV
                     IMVSASLPGASPETMASSVATPLERSLGRIAGVSEMTSSSSLGSTRIILQFDFDRDIN
                     GAARDVQAAINAAQSLLPSGMPSRPTYRKANPSDAPIMILTLTSDTYSQGELYDFAST
                     QLAPTISQIDGVGDVDVGGSSLPAVRVGLNPQALFNQGVSLDDVRTAISNANVRKPQG
                     ALEDDTHRWQIQTNDELKTAAEYQPLIIHYNNGGAVRLGDVATVTDSVQDVRNAGMTN
                     AKPAILLMIRKLPEANIIQTVDSIRARLPELQSTIPAAIDLQIAQDRSPTIRASLEEV
                     EQTLVISVALVILVVFLFLRSGRATIIPAVAVPVSLIGTFAAMYLCGFSLNNLSLMAL
                     TIATGFVVDDAIVVLENIARHLEAGMKPLQAALQGTREVGFTVLSMSLSLVAVFLPLL
                     LMGGLPGRLLREFAVTLSVAIGISLLVSLTLTPMMCGWMLKASKPREQKRLRGFGRVL
                     VALQQGYGKSLKWVLNHTRLVGVVLLGTIALNIWLYISIPKTFFPEQDTGVLMGGIQA
                     DQSISFQAMRGKLQDFMKIIRDDPAVDNVTGFTGGSRVNSGMMFITLKPRGERSETAQ
                     QIIDRLRKKLAKEPGANLFLMAVQDIRVGGRQANASYQYTLLSDDLAALREWEPKIRK
                     KLATLPELADVNSDQEDNGAEMNLIYDRDTMARLGIDVQAANSLLNNAFGQRQISTIY
                     QPMNQYKVVMEVDPRYTQDISALEKMFVINNEGKAIPLSYFAKWQPANAPLSVNHQGL
                     SAASTISFNLPTGKSLSDASAAIDRAMTQLGVPSTVRGSFAGTAQVFQETMNSQVILI
                     IAAIATVYIVLGILYESYVHPLTILSTLPSAGVGALLALELFNAPFSLIALIGIMLLI
                     GIVKKNAIMMVDFALEAQRHGNLTPQEAIFQACLLRFRPIMMTTLAALFGALPLVLSG
                     GDGSELRQPLGITIVGGLVMSQLLTLYTTPVVYLFFDRLRLRFSRKPKQAVTE"
     gene            22658..24073
                     /locus_tag="J1Y83_001837"
     CDS             22658..24073
                     /locus_tag="J1Y83_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBA7629987.1"
                     /translation="MTDLPDSTRWQLWIVAFGFFMQSLDTTIVNTALPSMAQSLGESP
                     LHMHMVIVSYVLTVAVMLPASGWLADKVGVRNIFFTAIVLFTLGSLFCALSGTLNELL
                     LARALQGVGGAMMVPVGRLTVMKIVPREQYMAAMTFVTLPGQVGPLLGPALGGLLVEY
                     ASWHWIFLINIPVGIIGAIATLMLMPNYTMQTRRFDLSGFLLLAVGMAVLTLALDGSK
                     GTGLSPLAIAGLVAVGVVALVLYLLHAQNNNRALFSLKLFRTRTFSLGLAGSFAGRIG
                     SGMLPFMTPVFLQIGLGFSPFHAGLMMIPMVLGSMGMKRIVVQVVNRFGYRRVLVATT
                     LGLSLVTLLFMTTALLGWYYVLPFVLFLQGMVNSTRFSSMNTLTLKDLPDNLASSGNS
                     LLSMIMQLSMSIGVTIAGLLLGLFGSQHVSVDSGTTQTVFMYTWLSMAFIIALPAFVF
                     ARVPSDTHQNVAISRRKRSAQ"
     gene            24070..25473
                     /gene="baeS"
                     /locus_tag="J1Y83_001838"
     CDS             24070..25473
                     /gene="baeS"
                     /locus_tag="J1Y83_001838"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     BaeS"
                     /protein_id="HBA7629988.1"
                     /translation="MKFWRPGITGKLFLAIFATCIVLLITMHWAVRISFERGFIDYIK
                     HGNEQRLQMLGDALGEQYAQHGNWRFLRNNDRFVFQILRSLEHDNNEDKPGPGMPPHG
                     WRTQFWVVDQNNKVLVGPRAPVPPDGTRRPIMVNGAEVGAVIASPVERLTRNTDINFD
                     RQQRQTSWLIVALSTLLAALATFPLARGLLAPVKRLVDGTHKLAAGDFTTRVAPTSED
                     ELGRLAEDFNQLASTLEKNQQMRRDFMADISHELRTPLAVLRGELEAIQDGVRKFTPE
                     TLASLQAEVGTLTKLVDDLHQLSMSDEGALAYQKAPVDLIPLLEVAGGAFRERFASRG
                     LKLQFSLPDSITVFGDRDRLMQLFNNLLENSLRYTDSGGSLKISAEQHDKTVRLTFAD
                     SAPGVSDDQLQKLFERFYRTEGSRNRASGGSGLGLAICLNIVEAHNGRIIAAHSPFGG
                     LSITVELPLERDLQREV"
     gene            25470..26192
                     /gene="baeR"
                     /locus_tag="J1Y83_001839"
     CDS             25470..26192
                     /gene="baeR"
                     /locus_tag="J1Y83_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BaeR"
                     /protein_id="HBA7629989.1"
                     /translation="MTELPIDENTPRILIVEDEPKLGQLLIDYLRAASYAPTLISHGD
                     QVLPYVRQTPPDLILLDLMLPGTDGLTLCREIRRFSDIPIVMVTAKIEEIDRLLGLEI
                     GADDYICKPYSPREVVARVKTILRRCKPQRELQQQDAESPLIIDEGRFQASWRGKMLD
                     LTPAEFRLLKTLSHEPGKVFSREQLLNHLYDDYRVVTDRTIDSHIKNLRRKLESLDAE
                     QSFIRAVYGVGYRWEADACRIV"
     gene            26372..26704
                     /locus_tag="J1Y83_001840"
     CDS             26372..26704
                     /locus_tag="J1Y83_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegP family protein"
                     /protein_id="HBA7629990.1"
                     /translation="MAGWFELSKSSDNQFRFVLKAGNGETILTSELYTSKASAEKGIA
                     SVRSNSPQEERYEKKTASNGKFYFNLKAANHQIIGSSQMYATAQSRETGIASVKANGT
                     SQTVKDNT"
     gene            26851..28212
                     /locus_tag="J1Y83_001841"
     CDS             26851..28212
                     /locus_tag="J1Y83_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-hydroxyuridine modification protein YegQ"
                     /protein_id="HBA7629991.1"
                     /translation="MFKPELLSPAGTLKNMRYAFAYGADAVYAGQPRYSLRVRNNEFN
                     HENLQLGINEAHALGKKFYVVVNIAPHNAKLKTFIRDLKPVVEMGPDALIMSDPGLIM
                     LVREHFPEMPIHLSVQANAVNWATVKFWQQMGLTRVILSRELSLEEIEEIRNQVPDME
                     IEIFVHGALCMAYSGRCLLSGYINKRDPNQGTCTNACRWEYNVQEGKEDDVGNIVHKY
                     EPIPVQNVEPTLGIGAPTDKVFMIEEAQRPGEYMTAFEDEHGTYIMNSKDLRAIAHVE
                     RLTKMGVHSLKIEGRTKSFYYCARTAQVYRKAIDDAAAGKPFDTSLLETLEGLAHRGY
                     TEGFLRRHTHDDYQNYEYGYSVSDRQQFVGEFTGERKGELAAVAVKNKFSVGDSLELM
                     TPQGNINFTLEHMENAKGEAMPVAPGDGYTVWLPVPQDLELNYALLMRNFSGETTRNP
                     HGK"
     gene            complement(28716..29033)
                     /locus_tag="J1Y83_001842"
     CDS             complement(28716..29033)
                     /locus_tag="J1Y83_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310918.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA7629992.1"
                     /translation="MKKIAAISLISVFLMSGCAVHNDETSIGKFGLAYKSNIQRKLDN
                     QYYTEAEASLARGRISGAENIVKNDAAHFCVTQGKKMQIVDLKTEGAGLHGVARLTFK
                     CGE"
BASE COUNT         6710 a         7791 c         7656 g         6936 t
ORIGIN      
        1 caaataatat gagataaata gtgctgcaac attgcatttt tgccccgatt tatccatgat
       61 cgaattgtga catttgtcat acaacgaata ggttttgtac ttactatgga atggattgcc
      121 gatccgtcta tctgggctgg gttaatcacg ctgattgtga tcgaactggt cctcggcatt
      181 gataacctgg tctttattgc catcctcgcc gaaaaactac cgcctaagca gcgcgaccgc
      241 gcacgggtta ccgggctcct gctggcgatg ttaatgcgcc tgttactgct ggcgtcaatc
      301 tcctggctgg tcaccctgac tcaaccactg ttcagcttcc gctcgtttac ctttagcgcc
      361 cgcgacttaa tcatgctgtt tggtggtttc ttcctgttgt tcaaagccac gatggagctg
      421 aacgaacggc tggaagggaa agacagcaat aaccccaccc aacgcaaagg tgcgaagttc
      481 tggggggtgg tggcacaaat tgtggtactg gacgccatct tctcactcga ctcggtgatt
      541 accgccgtcg ggatggtcga ccatttactg gtcatgatgg ccgccgtggt tatcgctatc
      601 agcctgatgt tgatggccag caagccgtta acccagttcg ttaacagtca cccgacgatc
      661 gttattctct gcttaagctt cctgttgatg attggcttta gcctggtggc ggaaggtttc
      721 ggcttcgtca ttccgaaagg ctacctgtac gctgccattg gtttctcggt gatgatcgag
      781 gcgctcaatc agttggctat ctttaaccgg cgacgttttc tttccgctaa ccagacactg
      841 cgccagcgga ctaccgaagc ggtaatgcgc ctgcttagcg ggcaaaaaga agatgcggaa
      901 ctggacgccg aaaccgcgtc catgctggtg gatcatggta accagcagat ctttaatccg
      961 caggaacggc ggatgattga acgggtactt aatcttaacc agcgtaccgt cagcagcatt
     1021 atgacgtcgc gccacgatat tgagcatatc gatctcaacg cgccggaaga cgagatccgc
     1081 cagttactgg agcgaaatca gcatacgcgg cttgtcgtta ctgatggcga tgacgcagaa
     1141 gatttgctcg gtgttgttca cgttatcgac ctgctacaac agttactgcg cggcgaaccg
     1201 ctcaacctgc gggtgttgat tcgccagccg ctggtattcc cggaaacctt gccgttgtta
     1261 cctgccctgg agcagttccg taatgcccgc acacactttg cttttgtggt ggatgagttt
     1321 ggctcggtgg aagggattgt gacattaagt gacgtcactg aaaccattgc cggtaactta
     1381 ccgaacgaag tggaagagat cgacgcccgc catgatattc agaagaatgc ggacggttcc
     1441 tggacggcga atggtcatat gccgctggaa gatctggtgc aatatgtgcc gttgccgctg
     1501 gatgaaaaac gtgaatatca caccattgcc gggctattga tggaatattt gcaacgtatt
     1561 ccaaaacctg gcgaagaagt tcaggtgggg aattatttgc ttaaaacgtt gcaggtggaa
     1621 agccatcgcg tgcagaaggt gcagattatt ccgctgcgta aggatggcga gatggagtac
     1681 gaggtgtgat ggcatgtagg cctgatgcga cgctttacgc gtcttatcag gcctacagga
     1741 caggtcacct ggtaacctac atcttctcca gcaacttctt cacatctttg ccattgcggg
     1801 aatctttatt ccgctcggcc cagtcgttaa gacggcgttt tgcttcatcc tgtagatgtt
     1861 tacgcagtaa ctgatccact tgcaggctgt aattaagttg ctgccagttg ccataaaccc
     1921 gcagcggtac tggcgtttct ttgaggaaat caatcagttt gctttccccg ttccagccac
     1981 ccacgacccg aatatcaaac tgggtgtcac aggtttgatc tgccagattc aacatgcctt
     2041 cccctgtcag caccagcaca ggcgattgac cttgcatgtc gtttaacgtc acgacgccat
     2101 ccttcaacgt caaatcggtg gtaaagcggt caagacgcgt cacgttatcg aagttttcag
     2161 cggccttcac atcaccacca ttacgttcta ccgcttgctg aatcatctgc tggaagttca
     2221 tcccttccat gcgtgtgtcg gtcatttcga catgtgcctg tccttgccag ttgtggcgga
     2281 atgcgtcggc atctatgtca gcaccagaga aatcaccagc cagtgacatc tttccggtca
     2341 acgaaatcgg atagttaaac gccttcagaa tggtgccaat ctcaacgttt tccagccgtg
     2401 gctggaagtt tatccgcgga tttattgatg tcgcgtccaa cgtgcccggc agtgaaacct
     2461 gtccaccgtt aagtttgccc tgcagttgag taatttccag caaacccgac ttgttggtca
     2521 tttgcgtggc aacatctgta aaattcattc cgcgccagcg cacgttactg gcctgcaaca
     2581 aaatatcagc cgtaaagcct tgcagtccct gataggccgg ttcatcaata cgcgaagaaa
     2641 tgaccgggcg cggcaacgtt gattggctct gcccttgctg cgcggcaccg ttctcaccat
     2701 tcgctgtttc attaaggggg atgaggttgt caagattcag ttgcgggaat tgcagtctca
     2761 gctgccattc cggtttctct gtcaacgtga cctgtgcttg cccgctcagc gtactgtcat
     2821 tggcggtcaa actaatttgg ttaaatgaaa ggcgtttatg tgactcctgc cactggacct
     2881 ggaaactccc ctgcccttga ataccttgtt ttggtaaatc ggcaccctgc aactgccagt
     2941 taatttgttc aatagccgcc attaaatcat gcggataatc agaagcatcc accgtaccgt
     3001 taagggatat cgtgagatcg cgctgatcgc gattaacccg cccggaaaac tcaaatgagc
     3061 cacgatgttg gggatcttgt tccatttgca ggcggatatt gcggattgtc acctgctcgt
     3121 catcttcatg ctggaacacc agaacgctgt ccgccacctt aagactggat atatcaaacg
     3181 accatccgcg atcgtctgac agatccggca aggtattgtc gcgcggtgca accggagcgt
     3241 cttcactgcg caccgcttcc gtctgcggcg tcagttggat cactgcccct tttagcatca
     3301 cctgcttaac gctcagttga tggctcagta gtggtaaaag cgccacgtcc agacgcatgt
     3361 tgtctgctcg aaccagtggc tggctcgcgc cctgggcggt gagagacatt cgcccggaga
     3421 ggatactaag ctgcggccag acgtgccaac gcagtggccc gtcgagctgc aattgataac
     3481 cgctacgtgc agcaacttgc ttgaccatat agtcgcggaa atcattcgga ttcaccagca
     3541 acactaacgc agataacccg gccaccagca cgaccaggag tatcatcagc gtcgtcagaa
     3601 atcgtctcat ggtatcctca atgggcctga attagtcttt atcgattcgg ctggctaccg
     3661 cgccctgctg gttgcgatat ttcgcatctt cacggcggtt gtaaggtcgc gccgccgggc
     3721 cggaaagcgg ctcaaagctc agcgcaccaa ttaacatgcc cggacgcagc gccagcggca
     3781 gcttaccgga gttgtagaac tccagcacaa tgcagccaga ccagcccgga tcgatacggt
     3841 gcgcggtgac gtgcaccatc agccccagac gcgccagtga ggaacgcccg tccagccatc
     3901 ccaccagatc ggctggcagc gtcaccgact ccagcgtcac cgccagcgcc agctctcctg
     3961 ggtgaagata gaacgcctcg ctttcattga gaacgatctc atcgctcatc acgcggtcaa
     4021 gcgcggcgct cacttcatct ttgggaccgc tcagatcgat aaacgctgcc gtgtgaccac
     4081 ggaaggtacg aaatttattg cccaggcgca catccaccgt cgcgccatta atacgctcca
     4141 ctggcggacg tgggttgatc gacaaacggc cttcatcaag ccaggcttca atatctcggt
     4201 cacacagacg catggcattt tctcctttcg tgcatcactc ccttaacgcc aattacgtca
     4261 agggcatacc aggttatcac tgaacggtac acaatttatc aagcttattc aaagaactga
     4321 cttattttcg ctttcaatat atcgatggcg atgcggtttt tcccgccgcg cggcacgata
     4381 atgtccgcat attgtttaga aggctcaatg aattgcagga acatcggacg cacggttttt
     4441 tgatattgcg ccatcactga atccattgaa cgcccacgct cgttaacgtc acgcttgatg
     4501 cggcgcatca ggcagatatc cagcggggta tcaacgaaaa ttgagaagtt aagttcgtca
     4561 cgcaaacgcg catccgtcag cagcaaaatg ccttcgagaa tgatgacctt cttcggctca
     4621 accgtcaccg tttctttcat acgcgtatgt tcaacatagc tgtaaaccgg caggtcaatt
     4681 gccgagccgc gtttcaacgc ttgtaaatgc tcaagcagca ggctgtgatc catcgcgctg
     4741 ggatggtcgt agttggtctt aacgcgttct tccatcgaca gatggctttg atctttgtaa
     4801 tagcagtctt cgggaattac gccgatgtgt tcatcaccga cttgctcacg taattcacga
     4861 taaagggtac tggcaataag actcttgccg gaagccgatg cgccagcgat accgataatg
     4921 acgcactgat gagactgatc agtcatatat ttagcgacct gattaacctg gatgttagga
     4981 agggggcgac gaagcgccaa acgcggcaat tatagggatt tcatccgcct gataccagtc
     5041 gaatagcgtt gccgcgcgct cagagttaat tgttgacgaa gaattcccgg tggcaaatta
     5101 cgttgatcag ttttatacaa ggtaaaaatt gttatacgca gttgcgcaaa ttatccgcct
     5161 ttacgtcact ttatgagcaa ttcgcatata aaatgtaaaa cttttgtact agcataaaca
     5221 cagaaacgaa tactggcgac ctggtcttgc ggataaagcg gtaatgagca aacaatcaca
     5281 gcatgtatta attgccctgc cccacccgct gcttcacctg gtcagtttag gtttagtctc
     5341 gtttatcttt acccttttct cgcttgagct ttcgcagttt ggcacccaac tcgccccact
     5401 gtggttcccg acgtccatca tgatggtggc gttttatcgc catgccgggc gcatgtggcc
     5461 gggaattgcg ctgagctgct cgctgggaaa tatcgccgca tccatcctgc ttttttccac
     5521 cagctcgctg aacatgacct ggacgaccat caatattgtt gaagccgtgg tcggggcagt
     5581 gctgctacgt aaattgctgc cgtggtataa ccccttgcaa aatctggctg actggctgcg
     5641 tctggcactc ggcagcgcca ttattccgcc tctgttgggg ggtgttctgg ttgtcctgct
     5701 gacgcccgga gacgatcctc tcagggcatt tttgatatgg gtactgtcag aatccatcgg
     5761 cgctctggca ctggtgccgc tgggattgtt atttaaacca cactatctac tgcgccatcg
     5821 caacccaagg ttgctttttg agtcgctgct cacgttagcc atcacgctga cgttaagctg
     5881 gctttcgatg ctgtatctgc cgtggccttt tactttcatt attgtgctgt tgatgtggag
     5941 cgccgtgcgc ctgccacgaa tggaagcctt tttgatcttc cttaccacgg tgatgatggt
     6001 gtcgctgatg atggccgcgg atccctccct gcttgctacg ccgcgtacgt acctgatgag
     6061 ccatatgccg tggctaccgt ttttgctgat cctgctgccc gccaacatca tgacgatggt
     6121 gatgtatgcc tttcgtgcgg aacgcaaaca catttccgaa agcgaaaccc gctttcggaa
     6181 cgccatggaa tattccgcca tcggcatggc attagtgggc accgagggac aatggctgca
     6241 atccaacaaa gcgctctgcc agtttctcgg ttacagtcag gaagagctgc gcggactcac
     6301 ctttcagcaa ctgacctggc cggaggatct caataaagat ctccaacagg ttgaaaagct
     6361 gataagcggt gaaataaaca cctattcaat ggaaaaacgt tactacaacc gcaatggcga
     6421 tgttgtctgg gcgttgcttg ccgtctcact ggtgcgccac atggatggca cgccgctcta
     6481 ttttatcgct cagattgaag acattaacga gctaaaacgc accgaacagg tcaatcaaca
     6541 actgatggag cgcatcacgc tggccaacga agcgggcggg attggcatct gggagtggga
     6601 gctgaagccg aatattttta gttgggataa gcggatgttc gagctgtatg aaattcctcc
     6661 gcatatcaaa ccgaactggc aggtgtggta cgagtgcgtg ctgccggaag atcgccagca
     6721 cgccgaaaaa gtgattcgtg attcgttgca atcacgctcg ccctttaaac tggaatttcg
     6781 cattgccgtg aaagatggca tccgccatat ccgcgccctc gccaaccggg tactgaataa
     6841 agaaggcgaa gttgaacgcc tgctcggcat taatatggat atgacggagg ttaaacagct
     6901 taacgaggca ttgtttcagg aaaaagagcg cctgcacatt acgctggact ccatcggcga
     6961 agccgtggtc tgtattgata tggcaatgaa aattaccttt atgaatccag tggcggagaa
     7021 gatgagcggc tggacgcagg aagaagcgtt aggtgttccg ctcctgacgg tgttgcatat
     7081 tacttttggc gacaacggac cattaatgga gaacatttac agtgctgaca cctcacgttc
     7141 cgcgatcgaa caagatgtgg tattgcactg ccggagcggc ggcagctacg acgtgcatta
     7201 cagtattacg ccgttaagta ctctggacgg cagcaatata ggctcggtac tggtgattca
     7261 ggacgttacc gaatcacgca aaatgctgcg ccagctgagc tacagcgcct cccatgatgc
     7321 actgacgcat ctcgccaatc gcgccagttt tgagaagcaa ctgcgtatcc tgctgcaaac
     7381 ggtaaacagt acgcatcagc gacatgccct ggtgtttatc gatcttgatc gctttaaagc
     7441 ggtgaatgac agcgccgggc atgcggcggg cgacgcttta ctgcgcgaac tggcgtcgtt
     7501 gatgctgagt atgctgcgct ccagcgacgt gctggcgcgg ctcggtggtg atgaatttgg
     7561 tctgctactg ccagactgca atgttgaaag cgctcgtttt atcgctacac gcattatcag
     7621 tgccgtgaat gactatcact ttatatggga aggacgtgtg catcgggtag gtgccagtgc
     7681 cgggattacc ttgattgatg acaacaatca tcaggcagct gaagtgatgt cgcaggctga
     7741 tatcgcctgt tatgcctcca aaaatggtgg ccggggccgg gtgacgattt acgaaccgca
     7801 gcaagctgcc gcacatagcg aacgggcagc gatatcgctt gatgaacagt ggcggatgat
     7861 taaagagaat cagttgatga tgatcgccca cggcatcgct tcgccacggc tcccggaagc
     7921 aagtaatttg tggctgattt cacttaagct ctggagttgc gaaagcgaga ttattgatga
     7981 acaaacattt cgtcgtagct tcagcgatcc ggcacttagc catgctcttg accaacgggt
     8041 atgccacgat tttttccagc aggccgcaaa agcggttgcc agtaaaggct taagcatcgc
     8101 cctccccctt tccgttgctg gtttgagtag cgccacgctg gtgaatgaac tgcttgagca
     8161 gctggaaaat agccctctac cagcacggtt attacatctg attattccgg cagaagcgat
     8221 tttcgatcac gcagaaagcg tgcaaaaact gcggctggcg ggatgtcgga tcgtattcag
     8281 tcaggtgggc cgagatctgc aaatcttcaa ctcgctgaaa gcaaatatgg cagattacct
     8341 gctacttgat ggtgagttat gcgccaacgt gcagggtaat ttgatggatg agatgctgat
     8401 tacgattatt caggggcacg ctcagcgact cgggatgaaa accatcgccg ggccagtcgt
     8461 tttaccctta gtgatggata cgctttcggg catcggcgtc gatctgattt atggtgatgt
     8521 gattgccaat gcccaaccgc tggatttgct ggtgaatagc agttatttcg cgattaactg
     8581 aggcgtgctt ccccatcgcc tgatgcgacg tatcaggcct acaattcgtc cgcttcgtct
     8641 ggttgccagc cttccgtata ccagatatgc aacagcgcat aagaacgcca gggtttccag
     8701 cgctcggcat aacggcggat ttgtgccggt gtcattcccg gaaatcgatg tttaatcaga
     8761 taatcatccg gcagaaaaac atctttcgcc tgccagccac gcaaagcaaa ataatttgcc
     8821 gtccagcgcc cgatacccgg aaaggtttgc agcgttttca tcgcttgttc tacgtcgccc
     8881 ggtattgtca ttggtaaggt gccctccagc gccgcatttg ccagatgaat cagcgcctct
     8941 gcccgtttca acggcatacc taacgctttt aatgcctgcg ggtcggctac tgccagccgc
     9001 tgaggcgtgg ggaagcaggc ataatccgga aaatcatcca gccgttcgcc ataaagctgt
     9061 gccactctgg aggtcaattt tgccgccatc gccacgctca ccagttggcc taaaatcgcc
     9121 cgcacgccct gctcaacagc gtcaacacaa ccaggtaaac gcaatcctgg ccgcgccgcg
     9181 cctaacttgc ccaacgcacc gttaacaatc tgcgggttac attgcagatc aaacaggcgg
     9241 ctcattttcg ccagacactc agcggcaaca ggttctaaac ctgcacttaa atttatgtgc
     9301 agagtatggc gggctatatc cggaatagca gtcaccacgc cgcgatactc gcctaccgcc
     9361 agactacggg cataataact gtccgcgacc gtttccacgc cgctcaccgc acgggcggcg
     9421 agaaatccca acatccacga ccagtcatac ggcggctgcc agttcagggt atacatcgct
     9481 tctccttgtt aatccgcttt cagcataaac gttattcaga cgttacgctt tgctttcata
     9541 ttccggttgt cgcgacggca acatttcgct aaagtcacgc cccttcttca ccggcatagg
     9601 gattatttcg tgtttattgg ttttgattac ggtacagcaa actgttcagt ggcggtcatg
     9661 cgcgacggta aaccgcattt gctaaaaatg gaaaacgaca gcacgctgct gccttcaatg
     9721 ctttgcgcgc caacgcgtga agcggtaagc gaatggctgt accgccatca tgatgttccg
     9781 gcagacgacg atgaaacgca ggcgctgttg cgtcgggcga ttcgttataa ccgcgaagaa
     9841 gatatcgatg ttacggcgaa aagcgtgcag ttcggtcttt cctcactggc acagtacatt
     9901 gatgatccag aagaagtgtg gtttgtgaaa tcaccaaaat cgttcctcgg tgccagcggc
     9961 ttaaaaccgc agcaggtagc gctgtttgag gatctggtct gcgcaatgat gttgcacatt
    10021 cgccagcagg cgcaggcaca gctgccagaa gcgattactc aggcggtgat tggtcgtccg
    10081 atcaacttcc aggggctggg cggcgatgaa gcaaacaccc aggcgcaagg gattctggaa
    10141 cgcgcggcga agcgtgccgg gtttaaggat gtggtattcc agtacgagcc ggtcgcggct
    10201 gggctggatt acgaagccac cttgcaggaa gaaaaacgag tgctggtagt ggatatcggc
    10261 ggtggtacga ctgactgttc attgctgctg atggggccgc aatggcgtgc gcgcctcgat
    10321 cgtgaagcca gcctgctggg tcatagtggt tgccgtattg gtggtaacga tctggatatc
    10381 gcactggcgt ttaagaacct gatgccgttg ctgggcatgg gtggcgaaac cgaaaaaggc
    10441 atcgccctgc cgattctgcc gtggtggaat gcggttgcga tcaacgacgt tcctgcgcag
    10501 agtgatttct acagtagtgc taacggtcgt ctgcttaacg atctggtacg cgatgcccgt
    10561 gaaccggaaa aagtggccct gttacagaaa gtctggcgtc agcgtttaag ctatcgcctg
    10621 gtgcgtagcg cagaagagag caaaatcgcc ctttcaagtg tagcggaaac ccgcgcctca
    10681 ctgccgttta tcagcgatga actggctacg ctgattagcc agcaagggct ggaaagcgcc
    10741 ctcagccagc cactggcgcg gattctggaa caggtgcaac tggcgctgga taacgcccag
    10801 gaaaaaccgg acgttatcta cctgaccggc ggtagcgccc gttctccgct gattaaaaaa
    10861 gcgctggcag aacagctgcc gggcattccg attgcaggcg gcgatgactt tggctccgtc
    10921 accgccgggc tggcacgctg ggcggaagtg gtgtttcgtt aatggaaaaa tggctcagca
    10981 acggcttaaa tctgcctgcg cctgagcctg tttttctgcc gcctgacgta gcggttccat
    11041 caacggtatg gtccgtgctg gcgcatgaag gcggaatcgc tgcaactctg tcgcaccgac
    11101 agtcagcgcc gattccagcc gatgtcgttt ggcggctatt tttgccatta ccgcttgtct
    11161 gtctgcaggc gttaccgctt cgttcggcct gaacacaaaa cggcgttcac agctcgcttt
    11221 ccagtcgata accgcctggg tcagatgatc accaaaacct tttacttgct taacgctccg
    11281 tcgggtaaca tccgctgctg tttcaatacc aaaggaccgt aacgccgctt tacgcgcagg
    11341 gccaacgccg ggaatagagg caacatcaat aaaaaatccc tccagaaact tctgcttctg
    11401 ccgttccctt gcggtgtcgt gaagtgctac cagagcgcgt ttctcttctt ccggtaaccc
    11461 gagaatttcg tccttcattt tttcgagcct cgcccgtttg gcgataaaac cttccagccc
    11521 gcccaactgc tggatctggc tgactaaatg gtcatagtcc attttcgcgc gattaaacgc
    11581 ctcacggcgt tgctggattt ctgctttgta cgctttgctt gtcaggctgc caataatcca
    11641 gatagccgcc agcacaggaa ccaaaataat ataacgcggt tctgcctgaa gaccgcaaag
    11701 caacagcgat aacgctgaca gtgcgatctc aatcagaatg atgtattcgc gccgtaacag
    11761 gcctgaagga agcggcctgc cagtcggttg gaaatgatcg ggtaatggca gttgcaatgc
    11821 tggcggtgct actgatgcca tcaccatcgc ccagactctc gccagcacaa aatcaccgcc
    11881 ggtggtaatg acctcttcgc cgagatcaat aaaatagata acgccttgat tatccagcgc
    11941 acaccacggg cagtcagtca aatgagcggg gtaaacatgc attgccgaaa cggtacattt
    12001 ctttaactgt tggcgtagta aatcaagtgc cgctacccac gcttttgccg tcggacgccc
    12061 ggttgccacg ccactttccg tgaatgcctg ctgaaacatg gcttcaacat cgcccggtaa
    12121 catcgacagc ggaatcgatc gtggcggcgg ttttaaacca cgtcgctgat tatctgacgc
    12181 gtaggcataa cggaaatggg caatatccgt ctccagcgca ttacccgcat cagagataag
    12241 cggtacaccg gaataaggat gccgcccacc aaacaagatg tgaaatatca gcaacgcaag
    12301 gccaaaatta tcgtgatttt ctgtgcgttc aaagccgaca aatgatggca tggtttgcag
    12361 ctctggcggc gtaaaatgcg acacgccgac ttcgcataaa tgcagtgtac cattggcatt
    12421 aatctgaaag gagtcgctat caatcaacac cactttgctg tcgcgaccta ccataaagct
    12481 gttctggttt acgtcaccca cgacgtgccc gtgctcatga acagtagcaa aagatgaagc
    12541 aatattgcgc gcaacataga gtagaaaatc ccacgcacaa tgagggtaac tctggcgacg
    12601 atgtgccggg ctatagatca tatgaatcgg ttctttacca gaaacttttg gcatcataaa
    12661 accgataact tttccgcctc gtccaccgtg aagcgttgcc tgcggccagg cgacataatt
    12721 caacaactgc gcgtcagctg tcgcagccat aaaggcaagt ttgtcctgtt ttaaggcggg
    12781 tggcggcgtg tgataaatct tggcgacgct atcgacaaac tcctcgatat cataaaccgc
    12841 accttcgccg cctttgccca gttcacggcc tggcgtaacg cactcaccag tagcagtata
    12901 taaagtgggt ttcatggtaa ccacatagcc agtgccaacg ttttatcatc atccgtacgc
    12961 tcgtttaccg ccgggctgga caaaaactgc tttaacaatt caggtaataa atcgagttgt
    13021 tcctgcgttg ctgacgccag tccattgaaa aacggggtaa agaaaggcac atgcggagaa
    13081 ttatccagca tatttaacgc cagtcgctga ataccatccg taaatgctgc aactttatgc
    13141 gcacgctcag tgctggtgaa agtttccagt ctggaaacgg catcttcatc ggtaatgaag
    13201 tgcgtcatat tggcatattc acccaccatc ggcgtgagcg gcagttgcaa accgtgcccc
    13261 aaatctacca ccacgccgcc atcgccaatc tgcataatca aggtgccatt agctgatgaa
    13321 atcaatccca aaaacgtgca ggcaaaatcg cgaaccgcta attctttggc ttccgcctcg
    13381 gcaaaaagtc gctgacgaat ggtcagcaca atgtccgttg cgaggacatc attaagcccc
    13441 aattccccgc cctgcacttt ttgcgacata taagccatcg cttcattgac ggcgagcatc
    13501 gcaccttcgc ccccctgcga gacgctgcct gcgccatcag cgacgaatac cgacaataat
    13561 ggctgctggt catttagcca ggcaacctgc atctggcagg catcctggca aggcaaatca
    13621 gcgctaatat gcgacgttcc aacggctgat gcatagacca gtcgccagct cacactgacg
    13681 tccagccttt tggcgcttcc agcacaactt ccgttcccgg cgtggagcgg gaaaccgaac
    13741 gcagcgaact ggataaccag ctaaacagtt cacggaattg taatccctgc agagacaaag
    13801 gctgacggac gctgatttgg gccagcgtct tcatatccgc cccctgaaca ccgatggtaa
    13861 agaaggcaaa cttcttatct tcttccccct gaaacacttt gttggcggca gcctgccact
    13921 catcggttgg tgcgccatcg gtgatcagga aaatccacgg acgataatag gaaataccat
    13981 tggcacgata ttcacgtttc cgctcctcaa ccatatccag ggatttggta atggcagcgc
    14041 ccattggtgt atcgccctgg gcaaacagga tgggcgggaa aaaattagcg gcactggtaa
    14101 agggttgttc cacatgcacc gggccgaacg tcacaatgcc gagttccact ctttttaagg
    14161 ccagcgaatc ggcaagcagc tcatcgcgaa aggtaaccaa tccggtgtta agttcattaa
    14221 tgggtctgcc gctcatcgag ccagaaacat ccagcagcaa aatgcagggg caacgtggtt
    14281 ccgggttact ggcaaaatcg ctggtggcaa acgtgatttg ttcgctcata agaaatcctt
    14341 tcttagagaa ttattgaggt ccggtatcat accgattgca ataatgaaat aagagcaaac
    14401 gaagaaactt tcagaattag gcccggcggc gcagaaaatt ttgctattcg cctcgcaaga
    14461 acggagagcc gtcgattgac actaagggcg gagtgacata atttcaggag tgagggtaga
    14521 gcggggtttc ccccgccctg gtagtcttag taagctggcc agctaatgac taagagcacc
    14581 acgatgatga gtagcttcat catgaccctt tccttattta aggccccttc ctcgggaggg
    14641 gctttcccgt ttcagcgtcc cgctgaaatc ttcggcttac ctcctttcgc cctgtatgca
    14701 gtctatcgct gacttgcgga taaaatagtt tcaagtgtga ttattgcgta acgactcgca
    14761 aagaatattt gctggcagga tcgcagactg caaagtctgc ggattgacaa ttggaccgtg
    14821 aaggcatact ttcaggagtg agggtagagc ggggtttccc ccgccctggt agtcttagta
    14881 tgctgggtag ctaatcacta agagcaccac gatgatgagt atcttcatca tgaccctttc
    14941 cttatttaag gccccttcct ccggaggggc tttcccgttt cagcgtcccg ctgaaatcgt
    15001 cggcttatct cctttcgcca tgcaagcagt ctatcgctaa cgcgtagata aaatagtttc
    15061 atgtgttatt actggatgcg tgctcgcaaa agtgcccgtc attcagacga ttcccgacag
    15121 tgtttcataa ttcctccatt tttctccctt attggctggc tacactagta tcattccgcg
    15181 aaacgtttca ggaagagaaa ctcttaacga tgaaaggcag ttataaatcc cgttgggtaa
    15241 tcgtaatcgt ggtggttatc gccgccatcg ccgcattctg gttctggcaa ggccgcaatg
    15301 actcccagag tgcaacccca ggggcgacga aacaagcgca gcaatcgcca gcgggtggtc
    15361 gccgtggtat gcgtgccggc ccattagctc cggttcaggc ggcgaccgca gtagaacagg
    15421 ctgttccgcg ttacctcacc gggcttggca ccattaccgc cgctaatact gttacggtgc
    15481 gcagccgcgt ggacggtcaa ctgatggcgt tacatttcca ggaaggccag caggtcaaag
    15541 ccggcgattt actggcagaa attgacccaa gccagttcaa agttgcatta gcacaagccc
    15601 agggccaact ggcaaaagat aaagccacgc tcgccaacgc ccgccgtgac ctggcgcgtt
    15661 atcaacaact ggcaaaaacc aatctcgtat ctcgtcagga actggatgcc caacaagcgc
    15721 tggtcagtga aaccgaaggt accattaagg ctgatgaagc aagcgtcgcc agcgcgcaat
    15781 tgcaactcga ctggagccgt atcaccgcac cagttgatgg tcgcgttggt ctcaagcagg
    15841 ttgatgttgg taaccaaatc tccagtggcg acaccaccgg gattgtggtg atcacccaaa
    15901 cacatcctat cgatttgctc tttaccctgc ctgaaagcga tatcgccacc gttgtgcagg
    15961 cgcaaaaagc cggaaaaccg ctggtggtag aagcctggga tcgcaccaac tcgaagaaat
    16021 taagtgaagg cacactgtta agccttgata accaaatcga tgccactacc ggtacgatta
    16081 aagtgaaagc acgctttaat aatcaggatg atgcgctttt ccctaatcag tttgttaacg
    16141 cgcgcatgtt agtcgacacc gaacaaaacg ccgtggtgat ccccaccgcc gccctgcaaa
    16201 tgggcaacga aggccatttt gtctgggtgc tgaatagcga aaacaaggtc agcaaacatc
    16261 tggtgacacc gggcattcag gacagtcaga aagtggtgat ccgcgcaggt atttctgcgg
    16321 gcgaccgcgt agtgacggac ggcattgatc gcctgaccga aggggcgaaa gtggaagtgg
    16381 tggaagccca gagcgccacc gcaccggaag agaaagccac cagccgcgaa tacgcgaaaa
    16441 aaggagcacg ctcctgatgc aggtgttacc cccgagcagc acaggcggcc cgtcgcgcct
    16501 gtttattatg cgtcctgtcg ccaccacgct gctgatggtg gcgatcttac tcgccgggat
    16561 tatcggttat cgcgccctgc ccgtttcggc gctgccggaa gtggactatc caaccattca
    16621 ggtggtcacg ctttatccag gtgcgagccc ggatgtcatg acctctgccg ttaccgcgcc
    16681 gctcgaacgc cagttcgggc agatgtctgg cctgaaacag atgtcgtcgc aaagttccgg
    16741 cggtgcgtca gttatcactt tacagttcca gctaacattg ccgctggatg tcgccgagca
    16801 ggaagtgcag gccgcgatta acgctgcgac caacctgcta ccgagcgatc tgcctaaccc
    16861 gccggtttac agcaaagtga acccggcaga tccaccgatc atgacgctcg ctgtcacctc
    16921 aaccgccatg ccgatgacgc aggtggaaga tatggtggaa acccgcgtcg cgcagaaaat
    16981 ctcgcagatt tccggcgtag ggctggtcac gctttccggc ggccagcgtc cggctgttcg
    17041 cgtcaaactt aacgctcagg cgattgccgc cctcggcctg accagcgaaa ccgtacgcac
    17101 agctattacc ggcgctaacg ttaactcggc aaaaggtagc ctcgacggcc cttcccgcgc
    17161 ggtcacgctt tccgctaacg accagatgca atccgccgaa gagtatcgcc agctaatcat
    17221 cgcctaccag aacggcgcac cgattcgtct gggcgatgtc gctactgtag agcaaggagc
    17281 agaaaacagc tggctcggcg catgggctaa caaagaacag gccattgtga tgaatgtcca
    17341 gcgccagccc ggtgctaaca ttatctccac cgccgacagc atccggcaga tgctaccaca
    17401 gctcaccgag agtctgccga aatcggtgaa ggtgacggta ctttctgacc gtaccaccaa
    17461 tatccgcgca tcggtcgacg atacccagtt tgagctgatg atggctatcg cgctggtagt
    17521 catgattatc tacctgtttt tgcgcaatat tccggcgacc atcattcccg gtgttgctgt
    17581 accgctgtcg ttaattggca cctttgcggt catggtgttt ctcgattttt caatcaataa
    17641 cctgacgttg atggcgttaa ctatcgccac cggattcgtg gtcgatgacg ccatcgtggt
    17701 gatcgaaaac atttcccgct atatcgaaaa aggcgaaaaa ccgttggcgg cggcgctcaa
    17761 aggcgcgggt gaaatcggct ttaccattat ctcgctgacc ttctcgctga ttgcggtgtt
    17821 gatccccctg ctgtttatgg gcgatatcgt cgggcgactg ttccgcgaat ttgctattac
    17881 cctggcagtc gcgattttga tctcagcggt ggtgtcgctg accctgacac cgatgatgtg
    17941 cgcacggatg ctcagccagg agtcgctgcg taaacagaac cgcttctccc gtgcctcgga
    18001 gaaaatgttc gacaggataa tcgccgccta tggtcgtgga ctggcgaaag tgctgaatca
    18061 tccgtggctg accttaagcg tggcgctcag cacgctgctg cttagcgtgc tgctgtgggt
    18121 gttcattccg aaaggtttct tcccggtgca ggacaacggc attattcagg gcactttgca
    18181 ggcaccgcaa tccagctcct ttgccaatat ggcccagcgg caacgccagg tcgcggacgt
    18241 gattttgcag gatccggcag tgcaaagttt gacctcattt gttggcgttg atggcactaa
    18301 cccgtcgctg aacagtgcgc gtttacagat caatctcaaa ccactggatg aacgagatga
    18361 ccgggtgcaa aaagtcatcg cccgtctgca aacggcggtg gataaagtgc cgggcgtcga
    18421 tctcttcctg caaccaacgc aggacctgac tattgatact caggtcagcc gcacccagta
    18481 ccagtttacc ttgcaggcca cgtcactgga tgcgctcagt acctgggtgc cacagttgat
    18541 ggaaaaactc cagcagctgc cacagctttc tgatgtctcc agcgactggc aggacaaagg
    18601 gctggtggcg tatgtcaatg tcgacaggga cagcgccagc cgtctgggga tcagcatggc
    18661 ggatgtcgat aacgccctgt acaacgcgtt tggtcagcga ctgatttcca ctatttatac
    18721 tcaggctaac cagtatcgcg tggtactgga acacaacacc gaaaataccc caggcctcgc
    18781 ggcgctggat accattcgcc tgaccagcag cgacggtggc gtggtgccgt taagctcaat
    18841 tgccaaaatt gagcagcgtt ttgcgccgct ttccatcaac catctggatc agttcccggt
    18901 aacgactatc tcctttaacg tgccggataa ctattcgctg ggcgatgcgg tgcaggcgat
    18961 tatggacaca gagaagacgc tgaatctgcc ggtggatatc accacgcagt tccagggcag
    19021 caccctcgcc ttccagtcgg cgctgggcag cacggtctgg ctgattgtcg cggcggtggt
    19081 ggcgatgtat atcgtgctcg gcattctgta cgagagcttt attcacccga tcaccattct
    19141 ctcgacgcta cccaccgcag gggtcggcgc gctgctggcg ttaatgattg ccggtagcga
    19201 actggatgtg attgccatta tcggcattat tttgctgatc ggtatcgtga agaagaacgc
    19261 catcatgatg atcgacttcg cgctggctgc tgagcgcgag caaggcatgt cgccgcgcga
    19321 agctatttac caggcttgtc tgttgcgttt tcgtccgatc ctgatgacca ctctggcggc
    19381 tctgcttggt gcgctgccgc tgatgttgag taccggggtc ggcgcggaac tgcgtcgtcc
    19441 gttaggtatc ggcatggtcg gcggtctgat tgtcagccag gtgctgacgc tgtttaccac
    19501 gccggtgatt tatctgctgt tcgaccgtct ggcattgtgg accaaaagcc gctttgcccg
    19561 tcatgaagag gaggcgtaag tgaagttttt tgccctcttc atttaccgcc cggtggcgac
    19621 gattttactg tcggtcgcca ttaccctgtg cggcatactg ggtttccgta tgctgccagt
    19681 cgccccgctg ccgcaggtcg attttccggt gatcatggtc agcgcctcgc tgcccggtgc
    19741 gtcaccagaa acaatggcgt cttccgttgc cacgccgctg gagcgctcac ttgggcgcat
    19801 tgccggagtc agcgaaatga cctccagcag ttcgctcggc agcacgcgca ttattttgca
    19861 gtttgatttt gaccgggata tcaacggcgc agcgcgcgat gtgcaggcgg cgatcaacgc
    19921 cgcgcaaagt ctgctaccca gcgggatgcc cagccgtccg acctatcgca aagcgaaccc
    19981 gtcggatgcg ccaattatga tcctcacgct gacatccgat acttattcgc agggtgaact
    20041 gtacgatttc gcctcgacac agctggctcc gacgatttcg caaatcgacg gtgtcggtga
    20101 tgtcgatgtt ggcggcagct cgctgcccgc cgtacgcgtc gggctgaatc cgcaagcgct
    20161 gtttaatcag ggcgtgtcgc tggacgatgt gcgcaccgcc atcagcaatg ccaacgtgcg
    20221 taaaccgcag ggggcgctgg aagatgatac tcaccgctgg cagatccaga ctaacgatga
    20281 actgaaaact gccgctgaat atcagccgct gattattcat tacaacaacg gcggcgcggt
    20341 tcgtctgggc gatgtggcga cggtgaccga ctcagtacag gatgtacgta acgccgggat
    20401 gaccaacgcc aaaccggcca ttttgctgat gatccgcaag ctgccggaag ccaatattat
    20461 ccagacggta gacagcatcc gcgcccgtct gccggaatta caatccacca ttccggcggc
    20521 gattgatctg caaattgccc aggatcgctc ccctactatc cgcgcgtcgc tggaagaggt
    20581 tgagcagacg ctggtaatct cagttgcact ggtgattctg gtggtgtttt tgttcctgcg
    20641 ttcgggtcgc gccaccatta ttcccgccgt tgcggtgccg gtttcgctga ttggtacgtt
    20701 tgcggcgatg tacctgtgtg gtttcagtct caataatctt tcgttaatgg cgctcaccat
    20761 cgccactggc tttgtggtgg atgacgccat cgtggtgctg gaaaacattg cccgtcatct
    20821 ggaagcgggg atgaaaccgc tacaggccgc gctacaaggt actcgcgaag tcggctttac
    20881 ggtgctgtcg atgagtctgt cactggtggc ggtgttcctg ccgctgctgt tgatgggcgg
    20941 attgccgggt cgcctgttac gcgagtttgc cgtgacgctt tctgtcgcca ttggtatttc
    21001 gttgctggtt tccctgacgt taacgccgat gatgtgcggc tggatgctaa aagccagcaa
    21061 gccgcgcgag caaaagcgac tgcgtggctt tggtcgcgtg ttggtagccc tgcaacaagg
    21121 ctatggcaag tcgctgaaat gggtgctcaa tcatacccgt ctggtgggcg tggtgctgct
    21181 tggcaccatt gcgctgaata tctggctgta tatctcgatc ccgaaaacct tcttcccgga
    21241 gcaggacacc ggcgtactga tgggcgggat acaggctgac cagagtattt cgtttcaggc
    21301 gatgcgcggt aagttgcagg acttcatgaa aattatccgt gacgatccgg cggtggataa
    21361 tgtcaccggc tttaccggcg gttcacgggt gaacagtgga atgatgttta tcacccttaa
    21421 accacgcggc gagcggagcg aaacggccca acagattatc gatcgcttgc gtaaaaaact
    21481 ggcgaaagaa ccgggagcga atctgttcct gatggcggta caggatattc gcgttggcgg
    21541 acgtcaggct aacgccagtt accagtacac gttgttatcc gacgacctgg cggcactgcg
    21601 cgaatgggag ccgaaaatcc gcaaaaaact ggcgacattg ccagagctgg ctgatgttaa
    21661 ctccgatcag gaagataatg gcgcggagat gaaccttatt tacgatcgcg acaccatggc
    21721 gcgattggga attgacgtgc aagccgccaa cagcctgtta aataacgcct tcggtcagcg
    21781 gcaaatctcg accatttacc agccgatgaa ccagtacaaa gtggtgatgg aagtggatcc
    21841 gcgctatacc caggacatca gtgcactgga aaaaatgttc gttatcaata acgaaggaaa
    21901 agcgatcccg ctgtcgtatt tcgctaaatg gcaaccggcg aacgctccac tgtcggtgaa
    21961 tcatcaggga ttatcggcag cctcgaccat ttcgttcaac ctgccgaccg ggaaatcgct
    22021 ctcggacgcc agtgcggcga tcgatcgcgc aatgacccag cttggtgtgc cttcgacggt
    22081 gcgcggcagt tttgccggaa cagcgcaagt gttccaggag acgatgaact cacaggtgat
    22141 cctgattatc gccgccatcg ccacggtata tatcgtactg ggtatccttt acgagagtta
    22201 tgtgcatcca ctgacgattc tctccaccct accctcggcg ggcgtcggag cgctgttggc
    22261 gctggagctg ttcaatgccc cgttcagcct aattgcgctg atagggatca tgttattaat
    22321 cggcatcgtg aagaaaaacg ccattatgat ggtcgatttt gcgcttgaag cccaacggca
    22381 cggtaacctg acgccgcagg aagctatttt ccaggcctgt ctgctgcgtt ttcgcccgat
    22441 tatgatgact accctggcgg cgctgtttgg tgcgctgccg ctggtattgt cgggcggtga
    22501 cggctcggag ttgcgccaac cattggggat caccattgtc ggcggtctgg tcatgagcca
    22561 gcttctgacg ctgtatacca cgccggtggt gtatctcttt ttcgatcgtc tgcggctgcg
    22621 tttttcgcgt aaacctaaac aagcggtaac cgagtaaatg acagatcttc ccgacagcac
    22681 ccgctggcaa ttgtggattg tggctttcgg cttctttatg cagtcgctgg acaccaccat
    22741 cgttaacacc gcgcttccct caatggcgca aagcctcggg gaaagtccgt tgcatatgca
    22801 catggtcatt gtctcttatg tactgaccgt ggcggtgatg ctgcccgcca gcggctggct
    22861 ggcggacaaa gtcggcgtgc gcaatatttt ctttaccgcc atcgtgctgt ttactctcgg
    22921 ttcactgttt tgcgcgcttt ccggcacgct gaacgaactg ttgctggcac gcgcgttaca
    22981 gggcgttggc ggcgcgatga tggtgccggt cggcaggttg acggtgatga aaatcgtacc
    23041 gcgcgagcaa tatatggcgg cgatgacctt tgtcacgctg cccggtcagg tcgggccgct
    23101 gcttggcccg gcgctcggcg gtctgctggt ggagtacgca tcgtggcact ggatcttttt
    23161 aatcaacatt ccggtgggga ttattggcgc gatcgccaca ctgatgttaa tgccgaacta
    23221 caccatgcag acgcggcgct ttgatctctc cggctttctg ttgctggcgg ttggcatggc
    23281 ggtattaacc ctggcgctgg acggcagtaa aggtacaggt ttatcgccgc tggcgattgc
    23341 tggcctggtc gcagttggcg tggtagcact ggtgctttat ctgctgcacg cccaaaataa
    23401 caaccgcgcc ctgttcagtc tgaaactgtt ccgcactcgt accttttcgc tgggcctggc
    23461 ggggagcttt gccggacgta ttggcagtgg catgttgccc tttatgacac cggttttcct
    23521 gcaaattggc ctcggtttct cgccgtttca tgccggactg atgatgatcc cgatggtact
    23581 cggcagcatg ggaatgaagc gaattgtggt acaggtagtg aatcgctttg gttatcgtcg
    23641 ggtactggtg gcgaccacgc tgggcctgtc gctggtcacc ctgttgttta tgactaccgc
    23701 tctgctgggc tggtactacg ttttgccgtt cgtcctgttt ttacaaggaa tggtcaactc
    23761 gacgcgtttc tcctctatga acaccctgac gctgaaagat ctcccggaca acctggcgag
    23821 cagcggcaac agcctgctgt caatgattat gcaattgtcg atgagtattg gcgtcaccat
    23881 cgccgggctg ttgctgggac tttttggttc gcagcatgtc agcgtcgaca gcggcaccac
    23941 acaaaccgtc tttatgtaca cctggcttag catggcgttt atcatcgccc ttccggcgtt
    24001 tgtctttgcc agagtgccga gcgatacgca tcaaaatgta gctatttcgc ggcgaaaaag
    24061 gagcgcgcaa tgaagttctg gcgacccggt attaccggca aactgtttct ggcgattttc
    24121 gccacctgca ttgtcttatt gatcacgatg cactgggcgg tacgtatcag ttttgagcgc
    24181 ggctttatcg attacatcaa gcatggtaat gaacaacggc tgcaaatgct cggcgatgcg
    24241 cttggtgagc agtacgccca gcacgggaac tggcgcttcc tgcgtaataa cgatcgcttt
    24301 gtatttcaga tcctacgttc actggagcat gataacaacg aagataagcc cggccccggt
    24361 atgccaccac acggctggcg cacgcaattt tgggtggttg atcaaaataa taaagtgctg
    24421 gttggcccgc gagcaccggt tccacccgac ggcacacggc ggcccattat ggtcaatggt
    24481 gcggaagttg gcgcggtgat cgcctcccct gttgaacgac tgacccgtaa tactgatatc
    24541 aattttgaca gacaacagcg gcaaaccagt tggctgattg tggctttatc taccttatta
    24601 gcggcgctgg cgacattccc actggcgcgc ggtttgctgg ctccggtaaa acgactggtg
    24661 gatggcacgc acaaactggc agcgggtgat ttcactactc gcgtagcccc caccagcgaa
    24721 gatgagttgg gcagactggc agaggatttc aatcaactcg ccagcacact ggagaaaaac
    24781 cagcagatgc gccgcgattt tatggccgat atttctcatg aactgcgcac gcctttagca
    24841 gtactgcgcg gcgaactgga agccattcag gatggtgtgc gtaaattcac gccggagacg
    24901 ctggcttctt tacaggcaga agtcggtacg ctgacaaaac tggttgacga tctccatcaa
    24961 ttgtctatgt ctgatgaagg cgctctcgcc tatcaaaaag caccggtaga tttgatccca
    25021 ctgctggaag tggcgggagg cgcatttcgc gaacgattcg ccagccgtgg cctgaaattg
    25081 caattttccc tgccagacag tattaccgta tttggcgatc gcgatcgttt aatgcagttg
    25141 ttcaataact tactggaaaa cagcctgcgc tacactgaca gcggcggcag cctgaaaatc
    25201 tctgccgaac agcacgacaa aacggtgcgc ctgacctttg ccgacagtgc gccgggtgtc
    25261 agtgacgatc agctacaaaa attgtttgaa cgtttttatc gcaccgaagg ttcccgcaac
    25321 cgcgccagcg gtggttccgg gctggggctg gcgatttgcc tgaacattgt tgaagcacat
    25381 aacggccgca ttattgccgc ccattcgcct tttggcgggc ttagcattac agtagagtta
    25441 ccgctggaac gggatttaca gagagaagta tgaccgagtt accaatcgac gaaaacacac
    25501 cgcgtatttt gatcgtggaa gatgaaccga agctggggca gttgctcatt gattatctgc
    25561 gtgctgcgag ctatgcgcca acgttgatta gccacggcga tcaggtactg ccatatgtgc
    25621 gccagacacc accggatctg atcctgttgg atctgatgct ccctggcacc gatggcctga
    25681 cgctgtgccg ggaaattcgt cgtttttctg acattccgat cgtgatggtg acggcaaaaa
    25741 tcgaagagat cgaccgcctg ctggggttgg agattggcgc agatgactat atctgcaagc
    25801 cgtacagccc acgggaagtg gtagcgcgcg tcaaaaccat tttgcgccgt tgcaaaccgc
    25861 agcgtgagtt gcagcaacag gatgctgaaa gcccgttgat tatcgacgaa ggtcgttttc
    25921 aggcttcctg gcgcggtaaa atgcttgacc tgacgcctgc agaatttcgt ctgctgaaaa
    25981 cgctctctca tgaaccagga aaagtgttct cccgcgagca gttgctgaac catctttatg
    26041 acgactaccg cgtggtaacc gaccgtacca tcgacagcca cattaaaaac ctgcgccgca
    26101 agctggaatc tctcgacgcc gaacagtcat ttatccgcgc cgtttacggc gtcggttacc
    26161 gctgggaagc cgacgcctgc cgcatcgttt aacattttta cctgccggag atagccttca
    26221 gaccagatcc ggcaacatta tcccacgcat ggtcagcaaa ctgacattat ctctccgccc
    26281 tggcaggcac cagaaaatcg ctcatacttt aattggcaaa cagtaccttt agatgctgtt
    26341 ttcgatacgc aatttcaatc aaggagtcat tatggctggt tggtttgaac tcagtaagag
    26401 cagtgataat cagttccggt ttgtgctaaa agcgggcaat ggtgagacta tcctcaccag
    26461 cgagctgtat acctcaaaag cctctgcgga aaagggcatc gcgtcggtgc gtagcaacag
    26521 cccgcaagaa gaacgctatg agaaaaaaac ggcaagcaat ggcaaattct atttcaatct
    26581 gaaagccgcc aatcatcaaa ttatcggctc cagccagatg tatgccaccg cgcaatctcg
    26641 tgaaaccgga attgcctccg ttaaagcaaa tggcacaagc cagacggtga aagacaatac
    26701 gtaattacga tgccgggcgt aggttgttgg cgtccggcgc tttaccagtt acccccacag
    26761 cgctacaatg cccgccctta aagtgggggc actcccctaa ccgcttcatc aggtgaagcg
    26821 gctctgacct gtcatcagaa cgagagaatt atgtttaaac cggaactcct ttccccggcg
    26881 ggaacgctga aaaatatgcg ttacgctttc gcttatggcg cagatgctgt ttatgcgggc
    26941 cagccgcgtt actccctgcg tgtgcgcaac aacgaattca accacgaaaa tcttcagctc
    27001 ggcatcaatg aagcccacgc gctggggaaa aagttttatg tcgtagtcaa cattgcaccg
    27061 cacaacgcca agctgaaaac ctttatccgt gacctgaaac cggtggtgga aatggggccg
    27121 gatgcgctga ttatgtccga tccagggctg attatgctgg tgcgtgaaca cttcccggaa
    27181 atgccaatcc acctctcggt acaggctaac gccgtaaact gggcgacggt gaaattctgg
    27241 cagcaaatgg gtctgacccg cgtgatcctc tctcgcgaac tgtcactgga agagattgaa
    27301 gagatccgca atcaggtgcc ggatatggag atcgaaatct tcgttcacgg cgcgctgtgc
    27361 atggcctact ccggtcgctg cctgctctct ggctatatca acaagcgcga cccgaaccag
    27421 ggcacctgca ccaacgcctg ccgctgggag tacaacgtcc aggaagggaa agaagatgac
    27481 gtcggcaaca tcgtacacaa gtacgagccg attccggtgc aaaatgttga gccgacgctg
    27541 ggtatcggcg cgccaaccga caaagtgttt atgatcgaag aagcccagcg tccgggcgaa
    27601 tatatgaccg cgtttgaaga tgagcacggc acttacatca tgaactcaaa agatctgcgc
    27661 gccatcgccc atgttgaacg cctgaccaaa atgggcgtgc attcgctgaa aatcgaaggc
    27721 cgtaccaaat ctttctacta ttgcgcacgc accgcgcagg tttaccgtaa agctatcgat
    27781 gacgccgctg cgggcaaacc gttcgatacc agcctgctgg aaactctgga aggtctggcg
    27841 catcgtggct ataccgaagg tttcctgcgt cgtcataccc acgacgatta tcagaactac
    27901 gaatacggtt attcggtttc tgaccgtcag cagtttgttg gtgagtttac cggtgagcgc
    27961 aagggggagc tcgcggcggt agcggtgaaa aataaattct ccgttggcga cagccttgag
    28021 ctgatgacgc cgcaaggcaa cattaacttt acccttgagc acatggaaaa cgccaaaggt
    28081 gaagcaatgc cggtcgcacc aggcgatggt tatactgtgt ggctcccggt gccgcaggat
    28141 cttgagctaa attacgcgct gctgatgcgt aatttctccg gggaaaccac gcgtaacccc
    28201 cacggtaagt gattaatttc gattattttt cccggatgga aaattcttag aaaccgatca
    28261 catacagctg catttattaa ggttatcatc cgtttcgctg aaaaacataa cccataaaat
    28321 gctagctgta ccaggaacca cctccttagc ctgtgtaatc tcccttacac gggcttattt
    28381 tttatgcata agccctatcc ctggtcaccg tcttccattg accacatcga tagaatcctc
    28441 cttcatagca cgatgctttt cacttatcga catcgtgctc ccacaggttc cggctacgca
    28501 cagccagaac gcgcatgttt gacgcttacc aaaaaatgaa cctaaagcat tggaatattt
    28561 ttgacatcat tttctgatgg ctgcataaaa taaaaattct gctttagttt catctatctg
    28621 tttgtcatta ttactcacat tcaataatgg tgttgaagaa tatcccatca cagataaaat
    28681 aaataaatat gtgcatggta gtcttgaatc tattctcact ctccacattt gaatgtcaga
    28741 cgagcgacgc catgtaatcc tgcaccttct gtcttcaggt caactatctg catttttttg
    28801 ccctgagtaa cacagaaatg agctgcatca ttttttacta tattttctgc accagatatt
    28861 ctacccctgg ctaaagaagc ttcggcttcg gtgtagtatt ggttatcgag tttacgctga
    28921 atattacttt tatatgcaag gccaaattta ccgatacttg tctcatcatt atgcacagca
    28981 caaccagaca taagaaaaac actaattaat gatatagcag ctatcttttt caccttacct
    29041 tcccccatta attaccaacg acattctcta gtgtttaaat ataataatgg cat
//
LOCUS       DADSYM010000050        28942 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2415
            SAMN08039579-rid11723173.denovo.050, whole genome shotgun sequence.
ACCESSION   DADSYM010000050 DADSYM010000000
VERSION     DADSYM010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039579
            Sequence Read Archive: SRR6362533
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 28942)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28942)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980162.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 03:39:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,484
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 4,347
            CDSs (with protein)               :: 4,347
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 43 of 85
            Pseudo Genes (incomplete)         :: 37 of 85
            Pseudo Genes (internal stop)      :: 19 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28942
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039579-rid11723173.denovo.050"
                     /strain="LGE2415"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8302 N 95.9628 W"
                     /collection_date="2015-08-24"
     gene            complement(353..1264)
                     /gene="gsiD"
                     /locus_tag="J1Y83_001843"
     CDS             complement(353..1264)
                     /gene="gsiD"
                     /locus_tag="J1Y83_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001236035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter permease GsiD"
                     /protein_id="HBA7629993.1"
                     /translation="MRLFNWRRQAVLNAMPLVKPDQVRTPWHEFWRRFRRQHMAMTAA
                     LFVILLIVVAIFARWIAPYDAENYFDYDNLNNGPSLQHWFGVDSLGRDIFSRVLVGAQ
                     ISLAAGVFAVFIGAAIGTLLGLLAGYYEGWWDRLIMRICDVLFAFPGILLAIAVVAVL
                     GSGIANVIIAVAIFSIPAFARLVRGNTLVLKQQTFIESARSIGASDMTILLRHILPGT
                     VSSIVVFFTMRIGTSIISAASLSFLGLGAQPPTPEWGAMLNEARADMVIAPHVAVFPA
                     LAIFLTVLAFNLLGDGLRDALDPKIKG"
     gene            complement(1267..2187)
                     /gene="gsiC"
                     /locus_tag="J1Y83_001844"
     CDS             complement(1267..2187)
                     /gene="gsiC"
                     /locus_tag="J1Y83_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter permease GsiC"
                     /protein_id="HBA7629994.1"
                     /translation="MLNYVIKRLLGLIPTLFIVSVLVFLFVHMLPGDPARLIAGPEAD
                     AQVIELVRQQLGLDQPLYHQFWHYISNAVQGDFGLSMVSRRPVADEIASRFMPTLWLT
                     ITSMVWAVIFGMAAGIIAAVWRNRWPDRLSMTIAVSGISFPAFALGMLLIQVFSVELG
                     WLPTVGADSWQHYILPSLTLGAAVAAVMARFTRASFVDVLSEDYMRTARAKGVSETWV
                     VLKHGLRNAMIPVVTMMGLQFGFLLGGSIVVEKVFNWPGLGRLLVDSVEMRDYPVIQA
                     EILLFSLEFILINLVVDVLYAAINPAIRYK"
     gene            complement(2205..3743)
                     /gene="gsiB"
                     /locus_tag="J1Y83_001845"
     CDS             complement(2205..3743)
                     /gene="gsiB"
                     /locus_tag="J1Y83_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter substrate-binding
                     protein GsiB"
                     /protein_id="HBA7629995.1"
                     /translation="MARAVHRSGLVALGIATALMASCAFAAKEVVVAVGSNFTTLDPY
                     DANDTLSQAVAKSFYQGLFGLDKEMKLKNVLAESYTVSDDGLTYTVKLREGIKFQDGT
                     DFNAAAVKANLDRASDPANHLKRYNLYKNIAKTEAIDPTTVKITLKQPFSAFINILAH
                     PATAMISPAALEKYGKEIGFHPVGTGPYELDTWNQTDFVKVKKFAGYWQPGLPKLDSI
                     TWRPVADNNTRAAMLQTGEAQFAFPIPYEQAALLEKNKNIELMASPSIMQRYISMNVT
                     QKPFDNPKVREALNYAINRPALVKVAFAGYATPATGVVPPSIAYAQSYKPWPYDPVKA
                     RELLKEAGYPNGFSTTLWSSHNHSTAQKVLQFTQQQLAQVGIKAQVTAMDAGQRAAEV
                     EGKGQKESGVRMFYTGWSASTGEADWALSPLFASQNWPPTLFNTAFYSNKQVDDFLAQ
                     ALKTNDPAEKTRLYKAAQDIIWQESPWIPLVVEKLVSAHSKNLTGFWIMPDTGFSFED
                     ADLQ"
     gene            complement(3763..5634)
                     /gene="gsiA"
                     /locus_tag="J1Y83_001846"
     CDS             complement(3763..5634)
                     /gene="gsiA"
                     /locus_tag="J1Y83_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter ATP-binding protein
                     GsiA"
                     /protein_id="HBA7629996.1"
                     /translation="MPHSDELDAGDVLAVENLNIAFMQEQQKIAAVRNLSFSLQRGET
                     LAIVGESGSGKSVTALALMRLLEQAGGLVQCDKMLLQRRSREVIELSEQSAAQMRHVR
                     GADMAMIFQEPMTSLNPVFTVGEQIAESIRLHQNASREEAMVEAKRMLDQVRIPEAQT
                     ILSRYPHQLSGGMRQRVMIAMALSCRPAVLIADEPTTALDVTIQAQILQLIKVLQKEM
                     SMGVIFITHDMGVVAEIADRVLVMYQGEAVETGSVEQIFHAPQHPYTRALLAAVPQLG
                     AMKGLDYPRRFPLISLEHPAKQEPPIEQKTVVDGEPVLRVRNLVTRFPLRSGLLNRVT
                     REVHAVEKVSFDLWPGETLSLVGESGSGKSTTGRALLRLVESQGGEIIFNGQRIDTLS
                     PGKLQALRRDIQFIFQDPYASLDPRQTIGDSILEPLRVHGLLPGKEAAARVAWLLERV
                     GLLPEHAWRYPHEFSGGQRQRICIARALALNPKVIIADEAVSALDVSIRGQIINLLLD
                     LQRDFGIAYLFISHDMAVVERISHRVAVMYLGQIVEIGPRRAVFENPQHPYTRKLLAA
                     VPVAEPSRQRPQRVLLSDDLPSNIHLRGEEVAAVLLQCVGPGHYVAQPQSEYAFMRR"
     gene            complement(5621..6586)
                     /gene="iaaA"
                     /locus_tag="J1Y83_001847"
     CDS             complement(5621..6586)
                     /gene="iaaA"
                     /locus_tag="J1Y83_001847"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HBA7629997.1"
                     /translation="MGKAVIAIHGGAGAISRAQMSLQQELRYIEALSAIVETGQKMLV
                     AGESALDVVTEAVRLLEECPLFNAGIGAVFTRDETHELDACVMDGNTLKAGAVAGVSH
                     LRNPVLAARLVMEQSPHVMMIGEGAENFAFAHGMERVSPEIFSTPLRYEQLMAAREEG
                     ATVLDHSGAPLDEKQKMGTVGAVALDLDGNLAAATSTGGMTNKLPGRVGDSPLVGAGC
                     YANNASVAVSCTGTGEVFIRALAAYDIAALMDYGGLSLAEACERVVMEKLPALGGSGG
                     LIAIDHEGNVALPFNTEGMYRAWGYAGDTPTTGIYREKGDTVATQ"
     gene            6790..8025
                     /gene="moeA"
                     /locus_tag="J1Y83_001848"
     CDS             6790..8025
                     /gene="moeA"
                     /locus_tag="J1Y83_001848"
                     /EC_number="2.10.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HBA7629998.1"
                     /translation="MEFTTGLMSLDTALNEMLSRVTPLTAQETLPLVQCFGRILASDV
                     VSPLDVPGFDNSAMDGYAVRLADIASGQPLPVAGKSFAGQPYHGEWPAGTCIRIMTGA
                     PVPEGCEAVVMQEQTEQTDNGVRFTAEARSGQNIRRRGEDISAGAVVFPAGTRLTTAE
                     LPVIASLGIAEVPVIRKVRVALFSTGDELQLPGQPLGDGQIYDTNRLAVHLMLEQLGC
                     EVINLGIIRDDPHALRAAFIEADSQADVVISSGGVSVGEADYTKTILEELGEIAFWKL
                     AIKPGKPFAFGKLSNSWFCGLPGNPVSATLTFYQLVQPLLAKLSGNTASGLPARQRVR
                     TASRLKKTPGRLDFQRGVLQRNADGELEVTTTGHQGSHIFSSFSLGNCFIVLERDRGN
                     VDVGEWVEVEPFNALFGGL"
     gene            8025..8774
                     /gene="moeB"
                     /locus_tag="J1Y83_001849"
     CDS             8025..8774
                     /gene="moeB"
                     /locus_tag="J1Y83_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-synthase adenylyltransferase MoeB"
                     /protein_id="HBA7629999.1"
                     /translation="MAELSDQEMLRYNRQIILRGFDFDGQEALKDSRVLVVGLGGLGC
                     AASQYLASAGVGNLTLLDFDTVSLSNLQRQTLHSDATVGQPKVESARDALTRINPHIA
                     ITPVNALLDDAELAAMIAKHDLVLDCTDNVAVRNQLNAGCFAAKVPLVSGAAIRMEGQ
                     ITVFTYQDGEPCYRCLSRLFGENALTCVEAGVMAPLIGVIGSLQAMEAIKLLAGYGKP
                     ASGKIVMYDAMTCQFREMKLMRNPGCEVCGQ"
     gene            complement(8850..9512)
                     /gene="fsa"
                     /locus_tag="J1Y83_001850"
     CDS             complement(8850..9512)
                     /gene="fsa"
                     /locus_tag="J1Y83_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415346.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HBA7630000.1"
                     /translation="MELYLDTSDVVAVKTLSRIFPLAGVTTNPSIIAAGKKPLEVVLP
                     QLHEAMGGQGRLFAQVMATTAEGMVNDARKLRSIIADIVVKVPVTAEGLAAIKMLKSE
                     GIPTLGTAVYGAAQGLLSALAGAEYVAPYVNRIDAQGGSGIQTVTDLHQLLKMHAPQA
                     KVLAASFKTPRQALDCLLAGCESITLPLDVAQQMISYPAVDAAVAKFEQDWLGAFGRT
                     SI"
     gene            9643..10542
                     /locus_tag="J1Y83_001851"
     CDS             9643..10542
                     /locus_tag="J1Y83_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl-radical enzyme activating protein"
                     /protein_id="HBA7630001.1"
                     /translation="MIFNIQRYSTHDGPGIRTVVFLKGCSLGCRWCQNPESRARTQDL
                     LYDPRLCLEGCELCAKIAPEVIERALNGLLIHREKLTPEHFTALTDCCPTQALTVCGE
                     VKSVEEIMATVLRDKPFYERSGGGLTLSGGEPFMQPEMATALLQASHEAGIHTAVETC
                     LHVPWKYIAPSLPYIDLFLADLKHVADAPFKQWTDGNAARALDNLKKLAAAGKKIIIR
                     VPLIQGFNADETSVKAITDFAADELHVSEIHFLPYHTLGINKYHLLNLPYDAPEKPLD
                     APELLDFAQQYACQKGLTATLRG"
     gene            10548..12980
                     /locus_tag="J1Y83_001852"
     CDS             10548..12980
                     /locus_tag="J1Y83_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl radical protein"
                     /protein_id="HBA7630002.1"
                     /translation="MTTLKLDTLSDRIKAHKNELVHIVKPPVCTERAQHYTEMYQQHL
                     DKPIPVRRALALAHHLANRTIWIKHDELIIGNQASEVRAAPIFPEYTVSWIEKEIDDL
                     ADRPGAGFAVSEENKRVLHEVCPWWRGQTVQDRCYGMFTDEQKGLLATGIIKAEGNMT
                     SGDAHLAVNFPLLLEKGLDGLREKVAERRSRINLTVLEDLHGEQFLKAIDIVLVAVSE
                     HIERFAALAREMAATETRESRRDELLTIAENCDLIAHQPPQTFWQALQLCYFIQLILQ
                     IESNGHSVSFGRMDQYLYPYYRRDVELNQTLDREHAIEMLHSCWLKLLEVNKIRSGSH
                     SKASAGSPLYQNVTIGGQNLVDGQPMDAVNPLSYAILESCGRLRSTQPNLSVRYHAGM
                     SNDFLDACVQVIRCGFGMPAFNNDEIVIPEFIKLGIEPQDAYDYAAIGCIETAVGGKW
                     GYRCTGMSFINFARVMLAALEGGRDATSGKVFLPQEKALSAGNFNNFDEVMDAWDTQI
                     RYYTRKSIEIEYVVDTMLEENVHDILCSALVDDCIERAKSIKQGGAKYDWVSGLQVGI
                     ANLGNSLAAVKKLVFEQGAIGQQQLAAALADDFDGLTHEQLRQRLINGAPKYGNDDDT
                     VDTLLARAYQTYIDELKQYHNPRYGRGPVGGNYYAGTSSISANVPFGAQTMATPDGRK
                     AHTPLAEGASPASGTDHLGPTAVIGSVGKLPTAAILGGVLLNQKLNPATLENESDKQK
                     LMILLRTFFEVHKGWHIQYNIVSRETLLEAKKHPDQYRDLVVRVAGYSAFFTALSPDA
                     QDDIIARTEHML"
     gene            13126..13941
                     /gene="ybiV"
                     /locus_tag="J1Y83_001853"
     CDS             13126..13941
                     /gene="ybiV"
                     /locus_tag="J1Y83_001853"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase YbiV"
                     /protein_id="HBA7630003.1"
                     /translation="MSVKVIVTDMDGTFLNDAKTYNQPRFMAQYQELKKRGIEFVVAS
                     GNQYYQLISFFPELKDEISFVAENGALVYEHGKQLFHGELTRHESRIVIGELLKDKQL
                     NFVACGLQSAYVSENAPEAFVALMAKHYHRLKPVKDYQEIDDVLFKFSLNLPDEQIPL
                     VIDKLHIALDGIMKPVTSGFGFIDLIIPGLHKANGISRLLKRWDLSPQNVVAIGDSGN
                     DAEMLKMARYSFAMSNAAENIKQIARYATDDNNHEGALNVIQAVLDNTSPFNS"
     gene            14093..15358
                     /locus_tag="J1Y83_001854"
     CDS             14093..15358
                     /locus_tag="J1Y83_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003570920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1479 domain-containing protein"
                     /protein_id="HBA7630004.1"
                     /translation="MASTFTSDTLPADHKAAIRQMKHALRAQLGDVQQTFNQLSDDIA
                     TRVAEINALKAQGDAVWPVLSYADIKAGHVTAEQREQIKRRGCAVIKGHFPHEQALGW
                     DQSMLDYLDRNRFDEVYKGPGDNFFGTLSASRPEIYPIYWSQAQMQARQSEEMANAQS
                     FLNRLWTFESDGKQWFNPDVSVIYPDRIRRRPPGTTSKGLGAHTDSGALERWLLPAYQ
                     RVFANVFNGKLAQYDPWHAAHRTEVEEYTVDNTTKCSVFRTFQGWTALSDMLPGQGLL
                     HVVPIPEAMAYVLLRPLLDDVPEDELCGVAPGRVLPISEQWHPLLIEALTSIPQLEAG
                     DSVWWHCDVIHSVAPVENQQGWGNVMYIPAAPMCEKNLAYAHKVKAALEKGASPGDFP
                     REDYETNWEGRFTLADLNIHGKRALGIDS"
     gene            complement(15325..16428)
                     /locus_tag="J1Y83_001855"
     CDS             complement(15325..16428)
                     /locus_tag="J1Y83_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015965190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HBA7630005.1"
                     /translation="MDKKLKIADIAARTGLSPSTVSRVLAGKANTSARAREQVLACAR
                     ELGVMDGMAAGRMLLNNLIIFAPQRAFDQRSDIFYYRVIQSISKALSPHEVRLRYCAL
                     EEFDSDPALFLSRMNEADTQAAILLGIDDPHIHDLAADLAKPCVLINCRDERMRLPAI
                     APDHRLIGAFAARYLFEMGHREVMNVMCLRRYTMEQRFAGIKESWRQHNLAFHHDRHL
                     LNISSFSAKEAEEKVGAWLDAANGKTLPTAFLVGGDFMAAGTVNALHRRGLRVPQDVS
                     VMSIDGFNLAAIEDVPLTAVHVPRDELGNEAVQMLQQRLIRPDAPVGSLLLHGRLAVR
                     ESVRRVRPGKGNTAVEREGLYDTQLSIPSARLP"
     gene            16723..18024
                     /locus_tag="J1Y83_001856"
     CDS             16723..18024
                     /locus_tag="J1Y83_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005225987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBA7630006.1"
                     /translation="MSQGINNDMTASKPRHIVKNLRWWILVLFLLGVTVNYITRNSLG
                     ILAPELKSNLGITTEQYSWIVGAFQLAYTLFQPLCGWLIDVIGLKLGFMICAGLWALA
                     CLMHAGAGNWVHLALLRFFMGGAEAAATPANAKTIGEWFPKSERPIAAGWAGVGFSIG
                     AMLAPPIIYFAHASFGWQGAFMFTGILAMLWVVLWWAFYHNPDKHPNLSKAELDFIRQ
                     DNEAPPIKLPFLTALKTVGKNKRFYGIAIPAFMAEPAWAVMSFWVPLYLSKTYGMDLK
                     QIALFAWLPFLAADLGSIASGYLTKLYTRLFGLSRVNSVVASSVSGAFLMVSLAVMAF
                     TNNPYIAIVLISIGGFGHQIISCMLSALVVESFDKGQMATVNGMRGSAAWIASFLFSL
                     IIGVTADKIGFNPLFIAMGFFDLIGAFFLVTFIAERRAKRA"
     gene            18039..20402
                     /locus_tag="J1Y83_001857"
     CDS             18039..20402
                     /locus_tag="J1Y83_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 31 protein"
                     /protein_id="HBA7630007.1"
                     /translation="MKTLKNWTLHKQSAHHVELLVDGQHILCLYVLEENLFRVLLKRK
                     GELALDRTWSIAPAQDVPWEGRSREDLSGFSLPAWTLIQHDDTVVIATESLRATVHQP
                     LWLEWHYRNNEGEWLPLVNDRPTSAYLLNAHGDGVAHYQSRRKDERFYGLGDKAGDLQ
                     RNGKRYEMRNLDAMGYNAVSTDPLYKHIPFTITRRDDISFGLFYDNLSSCWLDLGNEI
                     DNYHTAYRRWQAEAGDIDYYLFTGERVLDITKAFVRLTGKTLFGPKWSLGYSGSTMHY
                     TDAPDAQNQLMNFIRLCEEHAIPCDSFQLSSGYTSINGKRYVFNWNYDKVPQPKVMSQ
                     AFHNAGLHLAANIKPCLLQDHPRYNEVAERGLFIRDSETDEPERSSFWDDEGSNLDFT
                     NPQTIQWWQEGVTTQLLEIGIDSTWNDNNEFEVWDGEARCHGFGQTIAIKHIRPVMPL
                     LMMRASMEAQQRFAPEKRPYLISRSGCAGMQRYVQTWSGDNRTSWDTLRYNTRMGLGM
                     SLSGLYNLGHDVGGFSGDKPDPELFVRWVQNGVMHPRFTIHSWNDDHTVNEPWMYPGV
                     TPAIRSAIELRYRLLPYFYTLMWQAYADDEPMLRPTFLDHEHDVQTFEECDDFLLGRD
                     ILVASVVEQGERQRRVWLPDNETGWYDFYNGEWFSGGQWITLDAPLEKLPLMVRAGAG
                     LPLSKRITYVSAEQDDTRELKLFPLKGVGTTSGLLFEDDGKSWGYQTGNALWVEWEMV
                     CDGATINLKVNARGDYRPAWSALKVSLPVEEKRTLLVNGVEGSEWMR"
     gene            complement(20582..22177)
                     /locus_tag="J1Y83_001858"
     CDS             complement(20582..22177)
                     /locus_tag="J1Y83_001858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001522676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATPase"
                     /protein_id="HBA7630008.1"
                     /translation="MLVSSNVTMQFGSKPLFENISVKFGGGNRYGLIGANGSGKSTFM
                     KILGGDLEPTLGNVSLDPNERIGKLRQDQFAFEEFTVLDTVIMGHKELWEVKQERDRI
                     YALPEMSEEDGYKVADLEVKYGEMDGYSAEARAGELLLGVGIPVEQHYGPMSEVAPGW
                     KLRVLLAQALFADPDILLLDEPTNNLDIDTIRWLEQVLNERDSTMIIISHDRHFLNMV
                     CTHMADLDYGELRVYPGNYDEYMTAATQARERLLADNAKKKAQIAELQSFVSRFSANA
                     SKSRQATSRARQIDKIKLEEVKASSRQNPFIRFEQDKKLFRNALEVEGLTKGFDNGPL
                     FKNLNLLLEVGEKLAVLGTNGVGKSTLLKTLVGDLQPDSGTVKWSENARIGYYAQDHE
                     YEFENDLTVFEWMSQWKQEGDDEQAVRSILGRLLFSQDDIKKPAKVLSGGEKGRMLFG
                     KLMMQKPNILIMDEPTNHLDMESIESLNMALELYQGTLIFVSHDREFVSSLATRILEI
                     TPERVIDFSGNYEDYLRSKGIDG"
     gene            22396..23316
                     /gene="ldtB"
                     /locus_tag="J1Y83_001859"
     CDS             22396..23316
                     /gene="ldtB"
                     /locus_tag="J1Y83_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HBA7630009.1"
                     /translation="MNMKLKTLFAAAFAVVGFCSTASAVTYPLPTDGSRLVGQNQVIT
                     IPEGNTQPLEYFAAEYQMGLSNMMEANPGVDTFLPKGGTVLNIPQQLILPDTVHEGIV
                     INSAEMRLYYYPKGTNTVIVLPIGIGQLGKDTPINWTTKVERKKAGPTWTPTAKMHAE
                     YRAAGEPLPAVVPAGPDNPMGLYALYIGRLYAIHGTNANFGIGLRVSHGCVRLRNEDI
                     KFLFEKVPVGTRVQFIDEPVKATTEPDGSRYIEVHNPLSTTEAQFEGQEIVPITLTKS
                     VQTVTGQPDVDQVVLDEAIKNRSGMPVRLN"
     gene            complement(23374..24492)
                     /locus_tag="J1Y83_001860"
     CDS             complement(23374..24492)
                     /locus_tag="J1Y83_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000056422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anion transporter"
                     /protein_id="HBA7630010.1"
                     /translation="MSLPFLRTLQGDRFFQLLILVGIGLSFFVPFAPKSWPAAIDWHT
                     IITLSGLMLLTKGVELSGYFDVLGRKMVRRFATERRLAMFMVLAAALLSTFLTNDVAL
                     FIVVPLTITLKRLCEIPVNRLIIFEALAVNAGSLLTPIGNPQNILIWGRSGLSFAGFI
                     AQMAPLAGAMMLTLLLLCWCCFPGKALQYHTGVQTPEWKPRLVWSCLGLYIVFLTALE
                     LKQELWGLVIVAAGFALLARRVVLSVDWTLLLVFMAMFIDVHLLTQLPALQGVLSNVS
                     HLSDPGLWLTAIGLSQVISNVPSTILLLNYVPPSLLLAWAVNVGGFGLLPGSLANLIA
                     LRMANDRRIWWRFHLYSIPMLLWAALVGYVLLVMIPAG"
     gene            complement(24489..24956)
                     /locus_tag="J1Y83_001861"
     CDS             complement(24489..24956)
                     /locus_tag="J1Y83_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator MntR"
                     /protein_id="HBA7630011.1"
                     /translation="MSRRAGTPTAKKVTQLVNVEEHVEGFRQVREAHRRELIDDYVEL
                     ISDLIREVGEARQVDMAARLGVSQPTVAKMLKRLATMGLIEMIPWRGVFLTAEGEKLA
                     QESRERHQIVENFLLVLGVSPEIARRDAEGMEHHVSEETLDAFRLFTQKHGAK"
     gene            25142..25270
                     /gene="mntS"
                     /locus_tag="J1Y83_001862"
     CDS             25142..25270
                     /gene="mntS"
                     /locus_tag="J1Y83_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganase accumulation protein MntS"
                     /protein_id="HBA7630012.1"
                     /translation="MNEFKRCMRVFSHSPFKVRLMLLSMLCDMVNNKPQQDKPSDK"
     gene            25542..27125
                     /locus_tag="J1Y83_001863"
     CDS             25542..27125
                     /locus_tag="J1Y83_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase"
                     /protein_id="HBA7630013.1"
                     /translation="MNLTLKESLVTRSRVFSPWTAFYFLQSLLINLGLGYPFSLLYTA
                     AFTAILLLLWRTLPRVQKVLVGISSLVAACYFPFAQAYGAPNFNTLLALHSTNMEEST
                     EILTIFPWYSYLVGIFIFALGVIAIRRKKESEKPRWNTFDSLCLVFSVATFFVAPVQN
                     LAWGGVFKLKDTGYPVFRFAKDVIVNNNEVIEEQERMAKLSGMKDTWTVTAVKPKYQT
                     YVVVIGESARRDALGAFGGHWDNTPFASSVNGLIFADYIAASGSTQKSLGLTLNRVVD
                     GKPQFQDNFVTLANRAGFQTWWFSNQGQIGEYDTAIASIAKRADEVYFLKEGNFEADK
                     NTKDEALLDMTAQVLAQEHSQPQLIVLHLMGSHPQACDRTQGKYETFVQSKETSCYLY
                     TMTQTDDLLRKLYDQLRNSGSSFSLVYFSDHGLAFKERGKDVQYLAHDDKYQQNFQVP
                     FMVISSDDKAHRVIKARRSANDFLGFFSQWTGIKAKEINIKYPFISEKKAGPIYITNF
                     QLQKVDYNHLGTDIFDPKP"
     gene            complement(27175..27690)
                     /gene="ompX"
                     /locus_tag="J1Y83_001864"
     CDS             complement(27175..27690)
                     /gene="ompX"
                     /locus_tag="J1Y83_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein OmpX"
                     /protein_id="HBA7630014.1"
                     /translation="MKKIACLSALAAVLAFTAGTSVAATSTVTGGYAQSDAQGQMNKM
                     GGFNLKYRYEEDNSPLGVIGSFTYTEKSRTASSGDYNKNQYYGITAGPAYRINDWASI
                     YGVVGVGYGKFQTTEYPTYKHDTSDYGFSYGAGLQFNPMENVALDFSYEQSRIRSVDV
                     GTWIAGVGYRF"
     gene            complement(27743..27808)
                     /locus_tag="J1Y83_001865"
     CDS             complement(27743..27808)
                     /locus_tag="J1Y83_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA7630015.1"
                     /translation="METFCYMKWSVRHHKSRRVSH"
     gene            28043..28930
                     /gene="rhtA"
                     /locus_tag="J1Y83_001866"
     CDS             28043..28930
                     /gene="rhtA"
                     /locus_tag="J1Y83_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/homoserine exporter RhtA"
                     /protein_id="HBA7630016.1"
                     /translation="MPGSLRKMPVWLPIVILLVAMASIQGGASLAKSLFPLVGAPGVT
                     ALRLALGTLILIAFFKPWRLRFAKEQRLPLLFYGVSLGGMNYLFYLSIQTVPLGIAVA
                     LEFTGPLAVALFSSRRPVDFVWVVLAVLGLWFLLPLGQDVSHVDLTGCALALGAGACW
                     AIYILSGQRAGAEHGPATVAIGSLIAALIFVPIGTLQAGEALWHWSVIPLGLAVAILS
                     TALPYSLEMIALTRLPTRTFGTLMSMEPALAAVSGMIFLGETLTPIQLLALGAIIAAS
                     MGSTLTVRKESKIKELDIN"
BASE COUNT         7033 a         7944 c         7238 g         6727 t
ORIGIN      
        1 ttaaagaaat agccaacgtt tttctgaata agttcaaagc ggctatcggc acgttttacc
       61 gcgcgttccg ataccagttg atagttaatg atgaaaaaaa gaccaataaa tgacagtgct
      121 gccaataaaa ataaaattat cagccttatc tttatctttt cgtataaact cagcatgtta
      181 atacatttaa caaaataact gtctgaacgc tgtgccgctg caaaatagtc ccttgctgcg
      241 gctttttaac caaattaccc gattctacaa aatcagcaga aagttacaac acttgcttag
      301 ggcaataatt tcctgtgagg tgattaccct ttcaagcaat attcaaacgt aactatcctt
      361 taattttcgg atccagcgca tcgcgtaaac catcgcccaa caaattgaac gccagtacgg
      421 tcagaaaaat agccagggcc ggaaaaacag cgacatgcgg cgcgataacc atatccgctc
      481 gtgcctcatt gagcattgct ccccactctg gtgtcggcgg ctgcgcaccg aggccgagaa
      541 atgacaggct ggcggcagag ataatcgagg tgccaatgcg catggtgaaa aacaccacga
      601 tagaagagac ggtcccaggc aagatatgac gcaacaaaat ggtcatatcg ctggcaccaa
      661 tactgcgtgc tgactcgata aaggtttgct gtttcaacac cagcgtgttg ccgcgcacca
      721 gccgggcaaa cgcggggatg gaaaaaatgg cgactgcgat aatcacgtta gcaatgccgc
      781 ttcccaacac cgcaacaaca gcgatcgcca gtaaaatacc cgggaaggca aacagcacat
      841 cgcaaatacg catgatcagc cgatcccacc agccttcata atatccagcc agcaagccca
      901 gcaacgtccc gatcgccgca ccgataaata cggcaaacac gcccgccgcc agcgagattt
      961 gcgcaccaac caggacacgg ctgaaaatgt cgcgccccag tgaatcgaca ccaaaccagt
     1021 gctgcaaaga aggtccgtta ttcagattgt cataatcaaa ataattttcg gcgtcatagg
     1081 gagcgatcca gcgtgcaaaa atggccacca caatcaataa aataacgaac aatgcggcgg
     1141 tcatcgccat atgctgacgg cgaaatcgtc gccagaattc atgccacggt gtacgtacct
     1201 ggtcaggttt gaccagtggc atggcgttta acaccgcctg acgtcgccag ttaaatagtc
     1261 gcatccttac ttgtacctga tagccgggtt aatggcggcg taaagcacat ccaccactaa
     1321 gttgataaga ataaattcca gcgagaaaag cagaatttcc gcctgaatca ccgggtaatc
     1381 gcgcatttca acggagtcaa cgagtaagcg tccaagtccg ggccagttga aaactttctc
     1441 caccacgatg gaaccaccga gtaaaaatcc aaactgtaag cccatcatgg tcactaccgg
     1501 gatcatcgcg ttgcgtagcc cgtgtttgag gacaacccag gtttcgctca cccctttcgc
     1561 cctcgcggta cgcatataat cttcgcttaa aacgtcgaca aacgacgcgc gggtaaagcg
     1621 cgccatcacg gcggccactg ccgcgccgag cgtcagggag ggtaaaatgt agtgctgcca
     1681 actgtctgct cccacggtag gcagccagcc cagttcaaca gagaatacct gaattaaaag
     1741 catccccaga gcaaatgccg gaaacgaaat ccccgacacc gcaatagtca tactcaatcg
     1801 atccggccaa cggttacgcc agacggcggc gataattccc gccgccatac caaatatcac
     1861 cgcccagacc atactggtta tggtcagcca cagcgttggc ataaagcggc tggcaatctc
     1921 atcggcaacc ggacgacgcg acaccatcga caggccaaaa tccccctgca cagcattgct
     1981 gatatagtgc cagaactggt gatacagcgg ctgatccaac cccagctgct gacgcaccag
     2041 ttctataacc tgcgcatcag cttcgggccc ggcaattaat cgagccggat cgccgggcag
     2101 catatggaca aataaaaaca ccagcaccga gacgataaac agcgtcggaa tcaaccccag
     2161 taagcgtttg ataacgtaat taagcattcc actccctgcg ttgcttattg caaatccgca
     2221 tcttcaaagc tgaagcctgt gtctggcatg atccaaaaac cggtcaggtt tttactatgt
     2281 gccgacacca gtttttctac caccagcggg atccacggcg actcttgcca gatgatatcc
     2341 tgcgccgcct tatacaaacg ggtcttttcc gccggatcat tagttttcag tgcctgagcc
     2401 aggaagtcat ccacctgttt attgctgtaa aacgcggtat taaacagcgt cggtggccag
     2461 ttctgcgagg caaacagcgg cgatagagcc cagtctgctt cgccggttga agccgaccag
     2521 ccagtgtaga acatccgcac gccgctctct ttttgccctt taccttcaac ttctgccgcc
     2581 cgctgtccgg catccatcgc agtcacctgg gctttaatcc caacctgcgc taactgctgc
     2641 tgggtaaatt gcagcacttt ctgcgcagtg ctgtggttat gtgacgacca cagcgtggta
     2701 ctgaaaccgt tgggatatcc cgcctctttc agtaattcgc gcgctttcac tggatcgtaa
     2761 ggccacggtt tataactttg cgcgtaggcg atactcggcg gtaccacacc agtagctggc
     2821 gttgcatagc ccgcgaaggc cactttcacc agcgccgggc ggttaatggc gtaattcagc
     2881 gcctcacgga ccttcgggtt atcgaacggc ttttgcgtca cgttcatact gatataacgc
     2941 tgcataattg acggactggc catcaactcg atatttttgt ttttctccag cagtgcggcc
     3001 tgctcgtagg gaatggggaa agcaaactgc gcttcaccgg tttgcagcat tgccgcgcgg
     3061 gtgttgttat ccgccaccgg acgccaggtt atgctgtcca gtttgggcaa tcctggctgc
     3121 cagtaacccg cgaatttttt caccttcaca aaatcggtct gattccaggt atccagttca
     3181 tacggcccgg ttcccaccgg atgaaaacca atctccttgc catatttttc cagcgctgcc
     3241 ggtgaaatca tcgcggtcgc cggatgggca agaatattaa taaacgctga gaatggctgt
     3301 ttgagggtaa tctttaccgt tgtcggatcg atcgcttccg ttttggcaat attcttatac
     3361 aggttatagc gtttaagatg attcgccgga tcgctggccc ggtccagatt cgctttcacc
     3421 gccgctgcgt tgaaatcggt gccatcctgg aatttaatgc cttcccgcaa tttcacggtg
     3481 taagtaaggc cgtcatcaga aacggtgtaa ctctccgcca gcacgttttt cagtttcatc
     3541 tctttatcca gaccgaacag cccctggtaa aacgatttcg ctacggcctg agataacgtg
     3601 tcatttgcgt catacggatc gagcgtggtg aagttcgatc ctaccgccac caccacctct
     3661 ttggcagcga atgcacaaga tgccatcaac gctgtcgcaa tgcccagcgc cactaaccca
     3721 ctccggtgta cagctcttgc cattttattc tccgcctgaa tgttatctac gcatgaatgc
     3781 gtattctgat tgtggttgtg cgacgtaatg ccccggcccg acgcattgca acaagacggc
     3841 tgcaacttct tcgccacgca gatgaatatt gctgggaaga tcgtccgaca gcagtacacg
     3901 ctgcggtcgt tgtcgggacg gttcagcgac cggaactgcc gccagtaatt tacgcgtata
     3961 aggatgctgc ggattttcaa agaccgcgcg ccgtggacca atttcaacaa tttgcccgag
     4021 atacatcacc gccacacgat gactaatccg ctcaaccacg gccatatcgt gggagataaa
     4081 cagatacgca atgccgaaat cacgctggag atcgagcaac aagttgataa tctgcccgcg
     4141 aatagaaaca tccagcgccg agacggcttc gtcggcaatg atcacttttg gattcaatgc
     4201 caacgcgcga gcaatgcaga tgcgctggcg ctgaccgccg gaaaactcat gagggtaacg
     4261 ccaggcatgt tccggtaaca gacccacgcg ctccagcaac cacgcaacgc gtgcagccgc
     4321 ttctttacct ggcaataaac cgtgtacgcg cagcggttcg agaatcgaat caccgatggt
     4381 ctgacgcggg tccagcgaag cgtaagggtc ctgaaaaata aactgaatat cgcggcgcaa
     4441 tgcctgaagt ttgccgggtg acaaggtatc gattcgctga ccgttaaaga taatttcgcc
     4501 gccttgcgat tcgaccaggc gcagcaacgc ccgcccggta gtagatttac cgctgccaga
     4561 ctctcccacc agcgatagcg tttcaccagg ccagagatca aaactgactt tctcaacggc
     4621 atgcacttcc cgcgttacgc gattcaacaa accgctgcgc aaagggaaac gggtgaccag
     4681 attacgcacc cgtaaaacag gttcgccatc caccaccgtt ttctgctcga tgggtggctc
     4741 ctgtttcgct ggatgttcaa gcgatatcaa tgggaaacgt cggggataat ctaacccttt
     4801 catcgcacca agttgcggaa cagcagctaa cagcgcacgg gtataaggat gttgcggtgc
     4861 atgaaaaatc tgttcgacgc tacccgtttc caccgcctcg ccctgataca tcaccagtac
     4921 ccgatcggca atctctgcca ccacgcccat atcgtgagtg ataaagataa cgcccatcga
     4981 catctctttt tgcaatactt tgattaattg caggatctgc gcctgaatag tgacatccaa
     5041 cgcggtggtt ggctcatcgg caatcagcac cgccgggcgg catgacagcg ccatcgcaat
     5101 catcactcgc tggcgcatcc cgccagagag ttgatgcgga taacgtgaaa gaatggtttg
     5161 tgcctccgga atgcgtacct gatccagcat ccgcttcgcc tcgaccatcg cttcttcacg
     5221 actggcgttc tgatgcagac gaatagattc agcaatctgt tcacccacag taaataccgg
     5281 gttcagcgat gtcatcggct cctgaaatat catcgccata tccgccccgc gcacatggcg
     5341 catttgtgca gcactctgct cgctaagttc aattacttcg cgactgcgcc gctgcaacag
     5401 cattttatcg cactgcacta aaccgcccgc ctgttccaac aggcgcatca atgccagcgc
     5461 agtcactgac ttaccggagc cggattcgcc aacaattgcc agcgtctcac cacgttgcag
     5521 gctaaaagag agattgcgga ccgcagctat tttctgctgc tcctgcataa aggcaatatt
     5581 cagattttca accgccagca catcaccggc atcaagttca tcactgtgtg gcaacggtgt
     5641 cccctttttc acggtagata ccggtggttg gcgtatcgcc tgcgtagccc caggcgcgat
     5701 acattccttc ggtgttaaac ggtagcgcga cattcccttc atggtcgata gcgattaagc
     5761 caccgctacc gccaagcgca gggagttttt ccattactac ccgctcgcag gcttccgcga
     5821 gacttaatcc gccgtaatcc attaacgcgg cgatatcata tgccgccagc gcgcggatga
     5881 agacttcgcc cgtgccggta caggaaaccg ccacactggc gttattggca tagcatccgg
     5941 cccccactaa gggactatcg ccaactcgtc cgggtaattt attggtcatt ccgcccgtgg
     6001 acgtggctgc cgccagattg ccgtctaaat ccaacgccac ggcccctacg gtgcccattt
     6061 tctgtttttc atccagtggc gcaccgctat ggtcgaggac tgttgcccct tcctcgcgcg
     6121 ctgccattag ttgttcataa cgcaaaggtg tggagaaaat ctccggcgag acgcgctcca
     6181 tgccatgagc aaacgcaaaa ttttctgccc cttcgccaat catcatcaca tgtgggcttt
     6241 gctccatcac cagtcgggcg gcaagaaccg gattacgcag atgactaacg cccgccaccg
     6301 caccggcttt cagggtgtta ccatccatca cacaggcgtc cagttcatgg gtttcatcac
     6361 gcgtaaagac agcgccaatt ccggcgttaa acagtggaca ctcttccagc agacgcaccg
     6421 cttccgtcac cacatccagc gcactttcgc ccgctaccag cattttctgc ccggtttcaa
     6481 caatggcaga caacgcctcg atgtagcgta attcctgttg cagactcatc tgcgcgcggc
     6541 taattgcacc tgcgccacca tgaattgcaa tgactgcttt gcccatagta ttcgtccatt
     6601 aagggtggct cgccactttt gctataaata tcagaatttt tatgaatgac tcattcaatt
     6661 tttgaatata gaaagatgga tatggcatgt aaaggcagga gccagcgcta cgcccgaggt
     6721 ggcatacagt ctgtggcgtt tttctgacat aataggcaaa ttcgattttg cctccgcagg
     6781 agtgttttca tggaatttac caccggattg atgtcgctcg acaccgcgct taatgagatg
     6841 ctttctcgcg ttaccccact gaccgcccag gaaacgctgc cactggttca gtgttttggc
     6901 cgtattctgg cgagcgatgt cgtttcgcca ctggatgtcc cggggtttga taactccgct
     6961 atggacggct acgcggtgcg tttagccgat attgcctccg ggcaaccgct gcccgttgcc
     7021 ggtaaatcct ttgccggtca gccgtaccat ggtgaatggc ctgcgggtac ctgcattcgt
     7081 attatgaccg gtgcgccggt gccggaaggc tgcgaagcgg tggtgatgca ggagcagact
     7141 gaacaaacgg acaatggcgt gcgttttact gctgaagcgc gtagcgggca aaatattcgc
     7201 cgtcgcggtg aagatatctc tgcaggtgca gttgttttcc cggcggggac tcgcctgact
     7261 accgctgaac tgccagtgat tgcttcgcta gggattgccg aagttccggt gattcgtaaa
     7321 gtgcgtgtag cgcttttttc taccggtgat gaactccagt tgcccggtca gccgctgggc
     7381 gacggccaaa tctacgatac caaccgtctc gccgtacacc tgatgttaga acagttggga
     7441 tgcgaggtaa tcaacttagg gattatccgc gacgatcccc atgccctgcg cgccgcattt
     7501 attgaagccg acagccaggc ggatgtggtg atcagttccg gcggtgtttc agtgggtgaa
     7561 gcggattaca ccaaaacgat tcttgaagag ctgggggaga tcgccttctg gaaactggcg
     7621 attaaaccgg gtaaaccgtt cgcgttcggt aaactttcta atagttggtt ctgcggcctg
     7681 ccgggcaacc cggtttcagc aacgctgacc ttctatcaac tggtacagcc tttgctggca
     7741 aaactaagcg gcaacaccgc cagcggcctg cccgcgcgcc agcgtgtacg cacagcgtcc
     7801 cgcctgaaaa aaacgccagg acgccttgat ttccagcgcg gcgtgctgca acgcaacgcc
     7861 gatggcgaac tggaagtgac gaccaccgga catcagggtt cacatatatt tagctccttt
     7921 agcctcggca actgctttat cgtgctggaa cgcgatcgcg gcaatgttga cgtgggcgaa
     7981 tgggtggaag tcgaaccgtt taacgcgttg ttcggaggcc tgtaatggca gaactcagcg
     8041 atcaggagat gctgcgctac aaccggcaaa tcatcctgcg tggttttgat tttgacggtc
     8101 aggaagcgct gaaggattct cgcgtgttgg tggtggggct tggtggcctc ggctgtgcgg
     8161 catcgcaata tctggcaagc gccggtgtcg gcaacctgac gctgctcgac ttcgacacgg
     8221 tttcgctctc caatctgcaa cgccagacgc tgcacagtga tgccacggtc gggcaaccaa
     8281 aggtggaatc cgcccgtgac gccctgacgc ggatcaaccc acatatcgcg attacgccag
     8341 tcaatgcgct gcttgatgac gctgaacttg ctgcgatgat tgcaaaacac gatctggtgc
     8401 tcgactgtac ggataacgtt gcggtacgta atcaactgaa cgcaggctgt tttgccgcga
     8461 aggtgccgct ggtttccggc gcggcaattc gtatggaagg gcaaatcacg gtcttcactt
     8521 atcaggacgg cgaaccgtgc tatcgctgcc ttagccgttt gtttggcgag aacgcgttaa
     8581 cctgcgtaga agcaggcgtg atggcaccgt tgatcggggt gattggttcg ttgcaggcaa
     8641 tggaagcgat caaactgctg gcaggttatg gcaaaccggc cagcggaaaa atcgtgatgt
     8701 acgatgcgat gacctgtcag tttcgcgaaa tgaaactgat gcgtaatccg gggtgtgagg
     8761 tgtgcgggca gtaactgcca cctccaggcc agataagacg cggcactccg ccacgtcatc
     8821 agcaatttat gaggatgcag aacgtccggt taaatcgacg ttctgccaaa cgctcccagc
     8881 cagtcctgtt caaacttcgc cacagcggca tcaactgccg gatagctaat catctgttgt
     8941 gccacatcca gtggcagagt aattgattca caccctgcca gtaagcagtc cagcgcctga
     9001 cgcggggttt tgaaactcgc ggctaacact ttcgcctgcg gcgcatgcat tttcaataac
     9061 tggtgtaagt cggtcacagt ctgaataccg ctaccgccct gagcatcaat acgattaacg
     9121 taaggcgcaa catattccgc acctgccagc gccgacagta gcccttgtgc tgcgccatat
     9181 accgcggttc ccagcgtcgg aatcccttcc gattttaaca tcttaatagc tgccagcccc
     9241 tcagcggtca ccggaacttt caccacgata tccgcaataa tagaacgcaa tttacgtgcg
     9301 tcattcacca tcccttcagc agtggtagcc attacctggg caaatagacg cccctgaccg
     9361 cccatcgcct cgtgaagttg cggaagcaca acctccagcg gttttttacc cgctgcgata
     9421 atgcttgggt tagtggtcac acccgccagc ggaaaaatac gtgacagcgt cttcaccgca
     9481 acaacgtctg aagtatccag atacagttcc ataaccatcc tcaaaatgta ctttttgtct
     9541 gaatgtagac tatcacggga aaactctctg aacagttgac gcacatcaat ctaactttca
     9601 ttcgaaagta ttttaatgtt tgaacgaaac agagaggcaa ctatgatttt caatattcaa
     9661 cgctactcga cccatgatgg ccccggtatc cgcacggtcg tatttcttaa aggctgttcg
     9721 ctgggctgcc gctggtgtca gaacccggaa agccgcgccc gcacgcagga tctgctgtat
     9781 gacccacgac tctgtctgga aggctgtgaa ctgtgcgcta agatcgcacc ggaagtgatt
     9841 gagcgcgcgt tgaatggttt gcttattcat cgggaaaagt taaccccgga gcattttacg
     9901 gcgctaaccg actgctgccc gacacaggcg ttaaccgtgt gtggtgaagt gaaaagcgtt
     9961 gaggagatca tggcaaccgt tttgcgcgat aaaccgtttt acgagcgcag cggcggcggc
    10021 ttaacgcttt ctggcggtga accgtttatg cagccggaaa tggcgacggc gctgctgcaa
    10081 gccagccacg aggccggcat tcatactgcg gtagaaacct gtctgcatgt gccgtggaaa
    10141 tatatcgccc cttctctgcc ctatatcgat ctgtttcttg ccgatttaaa acacgttgcc
    10201 gacgcgccgt ttaaacagtg gaccgacggt aacgccgcca gggcgctgga taacctaaaa
    10261 aaactcgccg cagccggcaa aaaaatcatt atccgcgtgc cactgattca gggctttaat
    10321 gccgacgaaa cttctgtgaa agccattacc gattttgccg ccgacgagct gcacgttagc
    10381 gaaattcatt ttctgcccta ccacacactg ggcatcaaca aatatcactt acttaatctg
    10441 ccctatgacg ccccggaaaa accgcttgat gcgccagaac tgctcgactt tgcccagcag
    10501 tatgcctgcc agaaagggtt aaccgcgacc ttacgaggat aacaatcatg acaacactaa
    10561 aactggatac gctcagcgac cgcattaaag cgcacaaaaa tgagctggtg catattgtga
    10621 aaccgccggt ctgtaccgaa cgagcacaac actataccga gatgtatcaa caacatctcg
    10681 ataagccaat ccctgtacgt cgcgcgctgg cgctggcaca tcacctggcg aatcgcacta
    10741 tctggatcaa gcacgatgag ttgattatcg gcaaccaggc aagcgaagtt cgcgccgcgc
    10801 cgatcttccc ggaatatact gtctcgtgga tcgaaaaaga gattgatgat ctggcagatc
    10861 gtcccggtgc tggttttgcg gtgagcgaag agaacaaacg cgttctgcat gaagtgtgcc
    10921 cgtggtggcg cggtcagacg gtacaggatc gctgctacgg catgtttacc gatgagcaaa
    10981 aaggtctgct ggcgaccgga atcattaaag cggagggcaa tatgacctcc ggcgatgcgc
    11041 acctggcggt caatttcccg ctactgctgg aaaaaggtct tgatggtctg cgtgagaaag
    11101 tggcggaacg tcgctcgcgc atcaatctga cggttctgga agatctgcac ggcgagcaat
    11161 tcctgaaagc gattgatatc gtgctggtgg cagtcagtga acacattgaa cgtttcgctg
    11221 ccctggcgcg tgaaatggcc gcgaccgaaa cccgcgaaag ccgtcgcgat gaactgctga
    11281 cgatagcaga aaactgcgat cttatcgccc accagccgcc gcagactttc tggcaggcgc
    11341 tgcaactatg ttacttcatc cagttgattt tgcagatcga atctaacggt cactcggtat
    11401 cgtttggtcg tatggaccag tatctctatc catactatcg ccgtgacgtt gaactgaacc
    11461 agacactgga tcgcgaacac gccatcgaga tgctgcatag ctgctggttg aaattgctgg
    11521 aagtgaacaa aatccgctcc ggctcacact caaaagcctc tgcgggaagt ccgctgtatc
    11581 agaacgtcac cattggcggg caaaatctgg ttgatggtca accgatggac gcggtaaatc
    11641 cactctctta tgcgatcctc gaatcttgcg gtcgcctgcg ttcgactcag cctaacctca
    11701 gcgtgcgtta ccatgcgggt atgagtaacg atttcctcga cgcctgtgtt caggtgatcc
    11761 gctgcggctt cgggatgccg gcgttcaaca acgacgaaat cgtcatcccg gaatttatta
    11821 aactcggtat tgaaccgcag gacgcttacg actatgcagc gattggttgt atcgaaaccg
    11881 ccgtcggtgg caaatggggc tatcgctgta ccggcatgag ctttatcaac ttcgcccgcg
    11941 tgatgctggc ggcgctggaa ggcggtcgtg atgccaccag cggcaaagtg ttcctgccac
    12001 aagaaaaagc gttgtcagcg ggtaacttca acaacttcga tgaagtaatg gacgcatggg
    12061 atacgcaaat ccgttactac acgcgtaaat caatcgaaat cgaatatgtc gtcgatacca
    12121 tgctggaaga gaacgtacac gatattctct gctcggcgct ggtggatgac tgcattgagc
    12181 gagcgaaaag tatcaaacaa ggcggcgcga agtatgactg ggtgtctggc ttgcaggtcg
    12241 gtatcgccaa cctcggcaac agcctggcgg cagtgaagaa actggtattt gaacagggcg
    12301 cgattggtca gcaacagctg gctgccgcac tggcggatga cttcgacggt ctgactcacg
    12361 agcagctgcg tcagcggctg attaacggtg cgcctaagta cggcaacgac gatgatactg
    12421 tcgatacgct gctggctcgc gcttatcaga cctatatcga cgaactaaaa cagtaccata
    12481 acccgcgcta tggtcgtggt ccggttggcg gcaattatta cgcgggtacg tcatctatct
    12541 ccgctaacgt accctttggc gcgcagacga tggcaacgcc ggacggacgt aaagcgcata
    12601 ccccgctggc agaaggcgct agtccggcct ccggtactga ccatctcggc cctactgcgg
    12661 tcattggctc agtgggtaaa ctgcctacgg ccgcgattct cggcggcgtg ctgctcaacc
    12721 agaaactgaa tccagcaacg ctggagaacg aatctgacaa gcagaaactg atgatactgc
    12781 tacgcacctt ctttgaagtg cataaaggct ggcatattca gtacaacatc gtttcccgcg
    12841 aaacgctgct ggaagcgaaa aaacatccgg atcagtaccg cgatctggta gtgcgtgtcg
    12901 cgggctattc cgccttcttc accgcgctct ctccagacgc tcaggacgat atcatcgccc
    12961 gtactgaaca tatgctgtaa tctcctcagc ccggcggcgt cgtcgccggg ccaaatcatt
    13021 cacatcatca attccgctct cactttcatt cgaaatataa tttgtgctct gcgtcacatt
    13081 gttattacac tgttacggtt tcattttcga gccaggagca cacctatgag cgtaaaagtt
    13141 atcgtcacag acatggacgg tacttttctt aacgacgcca aaacgtacaa ccaaccacgt
    13201 tttatggcgc aatatcagga actgaaaaag cgcggcattg agttcgttgt cgccagcggt
    13261 aatcagtatt accagcttat ttcgttcttt cctgagctaa aggatgagat ctcttttgtc
    13321 gcggaaaacg gcgcactggt ttacgaacat ggcaagcagt tgttccacgg cgaactgacc
    13381 cgacatgaat cgcggattgt tattggcgag ttgctaaaag ataagcaact caattttgtc
    13441 gcctgcggtc tgcaaagtgc atatgtcagc gaaaatgccc ccgaagcatt cgtcgcactg
    13501 atggcaaaac actaccatcg cctgaaacct gtaaaagatt atcaggagat tgacgacgta
    13561 ctgttcaagt tttcgctcaa cctgccggat gaacaaatcc cgttagtgat cgacaaactg
    13621 cacatagcgc tggatggcat tatgaaaccc gtcaccagtg gttttggctt tatcgacctg
    13681 attattcccg gtctacataa agcaaacggt atttcgcggt tactgaaacg ctgggatctg
    13741 tcaccgcaaa atgtggtagc gattggcgac agcggtaacg atgcggaaat gctgaaaatg
    13801 gcgcgttatt cctttgcgat gagcaatgct gcggaaaaca ttaaacaaat cgcccgttac
    13861 gctaccgatg ataataatca tgaaggcgcg ctgaatgtga ttcaggcggt gctggataac
    13921 acatcccctt ttaacagctg acctttctac cggagctgtg cgctccggtt ttcccttctt
    13981 tttacatcct gtgcatcaca tcaaatcatt aaacttgaat taacttattt ataacttata
    14041 ttgtcacatg caaatcaatt tagtttcgaa tgaaagttaa tgcgaggtgg ttatggcttc
    14101 tacttttacc agcgacacat tgcctgccga tcacaaagca gctatccgtc agatgaagca
    14161 cgcgctgcgg gcgcagcttg gcgacgtcca gcagaccttt aatcagctaa gcgatgacat
    14221 tgccacgcga gtggctgaaa tcaacgcact caaagcacag ggcgatgccg tctggccggt
    14281 actgtcttat gccgatatca aagcaggtca tgttactgca gagcagcgcg aacagattaa
    14341 acgtcgcggt tgtgcggtga taaaaggcca tttcccccac gaacaagcgc taggctggga
    14401 tcagtcgatg ctggactatc tggaccgcaa ccgctttgac gaggtttaca aaggccccgg
    14461 cgataatttc ttcgggacgc tcagcgcttc acgtcccgag atttacccca tctactggtc
    14521 gcaggcgcaa atgcaggccc gccagagtga agaaatggcg aatgcgcagt cgtttctcaa
    14581 tcgtctgtgg acatttgaaa gtgatggaaa gcaatggttt aacccggatg tgagcgtcat
    14641 ctaccctgac cgtatccgcc gccgtccgcc cggaacgacc tccaaaggtc ttggagcgca
    14701 taccgactcc ggggcacttg aacgctggct gcttccagcg tatcagcgcg ttttcgccaa
    14761 cgtctttaat ggcaaactgg cgcaatatga tccctggcat gcggcacatc gtacggaagt
    14821 tgaagagtac acggtggaca acaccaccaa atgttccgtg tttcggacat tccagggctg
    14881 gacagcgctc tctgatatgc tgcctggtca ggggctgctg cacgtcgtgc ccattcctga
    14941 agccatggcg tacgtactgt tacgtccgct gcttgatgat gtgccggagg atgaactgtg
    15001 cggcgtagca cccggaagag tgctgccgat atcagagcaa tggcatccac tgttgattga
    15061 ggcgttaacc agcattccac agctggaagc cggagactcc gtctggtggc actgcgacgt
    15121 catccattcc gttgcccccg ttgaaaatca acagggttgg ggcaacgtga tgtacattcc
    15181 tgcggcaccg atgtgcgaga aaaatcttgc ctacgcgcac aaggtgaagg ccgcactgga
    15241 aaaaggcgca tcgccgggcg acttcccgcg cgaggactat gaaacaaact gggaaggacg
    15301 ctttacgctt gccgacctca acattcacgg taagcgagcg ctaggaatcg acagctgagt
    15361 gtcgtacagc ccttcccgtt ccacggcggt gttccctttt cctgggcgaa cccgccgcac
    15421 tgactcccgc accgccaatc gtccgtgcag cagcagcgag cccaccggag catccgggcg
    15481 aatcagccgc tgttgcaaca tttgcaccgc ttcattgcct agctcatcgc gtggcacatg
    15541 cacagccgtc aggggaacat cctcgattgc cgcaaggtta aagccgtcga tactcatcac
    15601 cgagacatcc tgtggtacgc gcagaccacg acgatgtagc gcatttaccg ttcccgccgc
    15661 cataaaatcc ccgccgacca gaaacgccgt cggcaacgtc ttgccattgg cggcatccag
    15721 ccacgctccg accttttctt ccgcttcttt agcgctaaaa ctgctgatat tcagcaaatg
    15781 acggtcatgg tgaaaggcca gattatgctg acgccatgat tctttaatcc ctgcaaaccg
    15841 ctgctccata gtgtagcggc gcaggcacat cacattcatc acctcccggt gacccatttc
    15901 aaacagatag cgggcggcga acgcgccaat taaccgatgg tccggcgcaa tcgccgggag
    15961 gcgcattcgt tcatcacggc agttgatcag cacacaaggc ttagcgagat cggcggccag
    16021 atcatggatg tgcggatcgt caatccccag caggatcgcc gcctgcgtat ctgcttcatt
    16081 catacgcgag agaaatagcg cgggatcgct gtcaaactcc tccagcgcgc agtaacgcag
    16141 ccgcacttca tgcggcgata acgctttgct gatgctctgg atcacacggt aataaaagat
    16201 atcagatcgt tggtcaaaag cgcgttgcgg cgcgaaaata atcaggttgt tcagcaacat
    16261 acgacctgct gccatgccat ccatcacgcc caactcacgc gcgcaggcca gcacctgttc
    16321 gcgagcgcgt gcactggtat tcgctttgcc agccagcacc cgcgaaaccg tactcggcga
    16381 tagcccggta cgtgcggcga tatcggcgat tttcagcttc ttgtccattt tgtgatctgc
    16441 ctctaattcg gaaatgacag aattttcatg agcaaaaaag cccattctgt ctggcattaa
    16501 atccatttca gtaatgtata tcagtcactc atggaaaaac gtgcacaaaa cccgctttct
    16561 ttcgctttta gggtctctta ctgtgaattg gtagaacaac ttccatacaa aaataatgga
    16621 aaaacgaaca ataaagactt tatttatcaa aaagttaaaa gtggtgtgac aactccattc
    16681 tgacgttgcc acacaaaccc tataccattc tgtggagtgc atatgagtca gggtatcaat
    16741 aacgatatga ctgccagtaa accgcgccat atcgtcaaaa atctgcgttg gtggatactg
    16801 gtcctttttt tgctcggtgt tactgtcaac tacatcacgc gtaactcgct gggtattctc
    16861 gcccccgaac tgaaaagcaa cctggggatt accaccgaac aatattcctg gattgtaggt
    16921 gctttccagc tcgcctatac cctattccaa ccgttgtgtg gatggctgat tgacgtcatt
    16981 ggcctcaaac tgggctttat gatctgcgcg ggactgtggg cactggcctg tctgatgcat
    17041 gcgggggcgg gaaactgggt acatctggcg ctgttgcgct tctttatggg cggtgcggaa
    17101 gccgcagcaa ccccagcaaa cgccaaaacc atcggggaat ggtttccaaa atcagaacgc
    17161 ccgatcgccg caggctgggc aggcgtcggt ttttccatcg gcgccatgct ggccccgccg
    17221 attatctatt ttgcccacgc ttcattcggc tggcagggcg catttatgtt cactggcatc
    17281 ctggcgatgc tgtgggtggt gctgtggtgg gcgttctatc acaacccgga taagcatccg
    17341 aacctcagca aagccgagct ggactttatc cgccaggata acgaagcgcc cccgattaaa
    17401 ttaccctttc tgaccgcact gaaaaccgtg ggcaaaaata aacgctttta cggtatcgct
    17461 atcccggcct ttatggcaga accggcgtgg gcggtgatga gtttctgggt gccgctatac
    17521 ctgtccaaaa cctatggcat ggacctgaag cagatcgcat tattcgcctg gttgccgttc
    17581 ctcgccgctg atcttggcag tattgccagc ggctatctga ccaaacttta cacccgcctg
    17641 tttgggctga gtcgtgtgaa ctccgttgtc gcatcatcgg tcagtggcgc attcttgatg
    17701 gtttcgctgg cggtgatggc ctttaccaat aacccgtata tcgccatcgt ccttatttcc
    17761 atcggcggct tcggtcacca gatcatctcc tgcatgctga gcgcactggt ggtggaatcc
    17821 ttcgacaaag gtcagatggc cactgttaac ggcatgcgcg gttctgccgc gtggatcgca
    17881 agtttcctgt tctccctgat tatcggggtt accgctgaca aaatcggctt taaccccctg
    17941 tttatcgcca tgggcttctt tgacctgatt ggcgctttct tcctggtcac ctttattgct
    18001 gaacgtcgcg caaaacgcgc ctgagagtgg atgtaaacat gaaaacgctg aaaaactgga
    18061 ccctgcacaa acaatctgcc caccacgttg aactgctggt cgatggccag catatcctgt
    18121 gcctgtacgt gctggaagaa aatctgttcc gcgtgctgtt aaaacgcaaa ggcgagctgg
    18181 cgctagatcg cacctggagc attgctccgg cgcaggatgt gccatgggaa gggcgcagcc
    18241 gcgaagatct cagcggcttt agcctgcccg cgtggacatt gatccagcac gacgacaccg
    18301 tggttatcgc caccgagtca ctgcgtgcaa ccgttcacca gccgctgtgg ctggagtggc
    18361 actatcgtaa caacgaaggc gaatggctgc cgttggtgaa tgaccgcccg accagcgctt
    18421 atctgctgaa tgcccacggc gatggcgtgg cacactatca gagccgtcgt aaagatgaac
    18481 gcttttatgg cctcggagat aaagccggcg acctgcaacg caacggtaaa cgttacgaga
    18541 tgcgtaacct cgacgcgatg ggctacaacg ccgttagtac tgacccgctg tacaaacaca
    18601 ttccattcac catcacccgc cgcgatgaca ttagcttcgg cctgttttat gacaacctca
    18661 gcagctgctg gctggatctg ggcaacgaga tcgacaacta ccacaccgcc tatcgccgct
    18721 ggcaggcgga agcgggtgat atcgactact acctgtttac cggcgaacgc gtacttgata
    18781 tcaccaaagc cttcgtacgc ctgaccggca aaaccctgtt cggaccgaaa tggagcctgg
    18841 gctacagcgg ttcaactatg cactacaccg atgcgccgga cgcacaaaat cagttgatga
    18901 actttatccg cctgtgtgaa gaacatgcga ttccctgcga ttcgttccag ctctcttccg
    18961 gttatacctc aattaacggc aagcgctatg tgttcaactg gaattacgac aaagtgccgc
    19021 agccgaaagt gatgagccag gcatttcaca atgctggttt gcacttggcc gccaatatca
    19081 agccgtgcct gttgcaggat cacccacgct ataacgaagt ggcagaaaga ggactgttca
    19141 tccgtgattc agaaacagat gaaccagaac gttccagctt ctgggatgac gaaggttcta
    19201 acctcgactt tactaatccg cagaccatcc agtggtggca ggaaggcgtg accacacagc
    19261 ttctggagat cggtatcgac tccacctgga acgacaacaa cgaattcgaa gtatgggacg
    19321 gtgaagcacg ctgtcacggc tttggtcaga cgatcgcgat caaacatatt cgcccggtga
    19381 tgccactgct gatgatgcga gcctcgatgg aagcccagca acgtttcgct ccggaaaaac
    19441 gtccgtactt gatctcccgc tccggctgcg cgggaatgca gcgctatgtg cagacctgga
    19501 gcggcgataa ccgcactagc tgggacactc tgcgctacaa cacccgcatg ggtctgggga
    19561 tgagcctctc cgggctgtat aacctcggtc acgacgtcgg cggtttctcc ggtgataagc
    19621 cggacccgga actgtttgta cgctgggtac aaaacggcgt gatgcatccg cgctttacga
    19681 ttcactcgtg gaacgatgac cataccgtaa acgaaccgtg gatgtatccg ggcgtcactc
    19741 cggcgatccg cagcgctatt gagttgcgct atcgcctgct gccctacttt tataccctga
    19801 tgtggcaggc atacgccgat gatgaaccaa tgctgcgtcc gactttcctt gaccacgagc
    19861 acgacgttca gacttttgaa gagtgtgatg acttcctgct tggtcgcgat attttggttg
    19921 ccagcgtcgt agagcaaggc gagcgccagc gccgtgtatg gctgccagat aacgaaactg
    19981 gctggtacga tttctacaac ggcgaatggt tcagtggcgg tcagtggatc accctcgacg
    20041 caccgctgga gaaactgccg ctgatggtgc gtgccggggc tggtctaccg ctcagcaaac
    20101 gcatcaccta tgtgagcgca gaacaagacg atacccgcga actgaaactg ttcccgctga
    20161 aaggtgtggg cacaacgtcc ggcctgctgt ttgaagacga tggcaaaagc tggggctacc
    20221 aaactggtaa tgcgctttgg gtggaatggg aaatggtttg cgatggggcg accatcaacc
    20281 tgaaagtcaa cgcgcgcggg gattatcgac ctgcatggag tgcactgaaa gtgtcattac
    20341 cagtggaaga aaaacgcacg ctgctggtca atggtgttga agggagtgaa tggatgcgtt
    20401 aaatcattac cggggccggg aggcgactgc gccttacccg gtccagccat cgaatccttt
    20461 acctcagtat ttcacgctgc aggaaggcag caaacctgta aaccccggga gcttaaataa
    20521 gtaagtgacc ggggtgaaca ggtgaagcca atgcatctgc cgcatgaaag acggcgggaa
    20581 attaaccgtc gatcccttta ctacgcaggt aatcttcgta attaccgcta aagtcgatca
    20641 cgcgttccgg ggtgatttcc agaatacggg tcgccaggga gcttacaaac tcacggtcgt
    20701 gagaaacaaa gatcagcgtg ccctgataca gttccagcgc catgttcagc gactcaatgg
    20761 attccatatc caggtggttg gtcggttcgt ccatgatcag aatgttcggc ttctgcatca
    20821 ttaacttacc aaacagcatc cgcccttttt ccccaccgga aagcactttg gcaggctttt
    20881 tgatgtcgtc ctgactgaac agcaaacgac cgagaatgct gcgtaccgcc tgctcgtcat
    20941 cgccttcctg cttccactga ctcatccatt cgaacacggt cagatcattt tcaaactcat
    21001 attcgtggtc ctgagcgtag taaccaatgc gcgcgttttc agaccatttt acggtgccgc
    21061 tgtccggttg cagatcgccc accagcgttt tcagcagcgt tgatttaccg acgccgttgg
    21121 tacccagaac cgccagtttt tcacccactt ccagcagcag gttgagattt ttaaacagtg
    21181 gaccgttatc aaacccttta gtcagacctt ccacttccag cgcgttacgg aacagtttct
    21241 tatcctgctc aaaacggatg aacgggttct gacggctgga ggctttcacc tcttccagtt
    21301 tgattttatc aatctggcgc gcgcgcgaag tcgcctggcg agatttcgag gcgttggcac
    21361 taaagcggct aacgaaagat tgcagctcag caatctgcgc tttcttcttg gcgttatcag
    21421 ccagcagacg ctcacgcgcc tgggtcgccg ccgtcatgta ctcatcgtag ttgcccggat
    21481 aaacgcgcag ctcgccgtaa tccagatccg ccatgtgggt acaaaccatg ttaaggaagt
    21541 gacggtcgtg cgagatgatg atcatggtgc tgtcacgctc gttcagcacc tgttccagcc
    21601 agcgaatggt gtcgatgtcg aggttgttgg tcggttcgtc gagcaggaga atgtccggat
    21661 cagcaaacag cgcctgcgcc agcagcacac gcagcttcca gccaggagca acttcactca
    21721 tcgggccgta gtgctgttcc actggaattc ccacgccaag caacagttca ccggcacgag
    21781 cttccgcaga gtaaccgtcc atttcgccgt atttcacttc cagatcggcc actttatagc
    21841 cgtcttcttc actcatttcc ggcaaagcat agatgcggtc acgctcctgc ttcacttccc
    21901 acaactcttt atgccccatg atcaccgtat ccagcacagt gaactcttca aaggcaaact
    21961 gatcctgacg cagcttacca atgcgctcgt tgggatcgag ggaaacgtta cccaacgtcg
    22021 gctctaagtc gccgccgagg atcttcataa aggtggattt accactaccg ttcgcgccaa
    22081 tcaggccgta acggttgccg ccgccgaatt tgacggaaat gttttcaaac aacggcttac
    22141 tgccgaactg catggtgacg ttactggaaa ctaacacagg cgtatcctga aaagagatat
    22201 gacaaaccgc gcattatgcc ataagtctga aagaagatcc tgctcctcgc ttaaggtcta
    22261 cactcaagcc aaaggcaaaa aatgtgattt cgtacacatc tgatttcact gtgagctgga
    22321 atgaacttat aatgcacttc caatactcta atattctcaa cccaatggcc tgccaggcac
    22381 aaaatctcgc ttaacatgaa tatgaaattg aaaacattat tcgcagcggc cttcgctgtt
    22441 gtcggctttt gcagtaccgc ctctgcggta acttatcctc tgccaaccga cgggagtcgc
    22501 ctggttggtc agaatcaggt gatcaccatt cctgaaggta acactcagcc gctggagtat
    22561 tttgccgccg agtaccagat gggactttcc aatatgatgg aagcgaaccc gggtgtggat
    22621 accttcctgc cgaaaggcgg tactgtactg aacattccgc agcagctgat cctgccggat
    22681 accgttcatg aaggcatcgt cattaacagt gcagagatgc gtctgtatta ctatccgaaa
    22741 gggaccaaca ccgttatcgt gctgccgatc ggcattggtc agttaggcaa agacacgcct
    22801 atcaactgga ccaccaaagt tgagcgtaaa aaagcaggcc cgacctggac gccgaccgct
    22861 aaaatgcacg cagagtaccg cgctgcgggc gaaccgcttc cggcggtcgt tccggctggt
    22921 ccggataacc cgatggggct gtatgcactc tacatcggtc gcctgtacgc tatccatggc
    22981 accaacgcca acttcggtat cggcctgcgt gtaagtcatg gttgtgtgcg tctgcgtaac
    23041 gaagacatca aattcctgtt cgagaaagta ccggtcggta cccgcgtaca gtttattgat
    23101 gagccggtaa aagcgaccac cgagccagac ggcagccgtt atattgaagt ccataacccg
    23161 ctgtctacca ctgaagccca gtttgaaggt caggaaattg tgccaattac cctgacgaag
    23221 agcgtgcaga cagtgaccgg tcagccagat gttgaccagg ttgttctgga tgaagcgatc
    23281 aaaaatcgtt ccgggatgcc ggttcgtctg aattaatctt caaaacgtaa agcaaaggcg
    23341 gactcataat ccgccttttt aatttgacag accttatccc gccgggatca taaccaacaa
    23401 aacatatccc accagcgccg cccacaacag catcggtatc gaataaagat gaaaacgcca
    23461 ccagatacgg cgatcgttcg ccatccgtaa cgcaatcaaa tttgccagcg atccgggtaa
    23521 taaaccaaag ccccccacgt ttaccgccca tgccagtaat aaagacggtg gcacataatt
    23581 gagcagcaaa atggtactcg gcacgttact gatcacctgc gataaaccaa tcgccgttaa
    23641 ccataatccg gggtcggata aatgactcac gttacttaac accccctgca aagctggcag
    23701 ttgggttagt aaatggacgt cgataaacat cgccataaac accagcagca gcgtccagtc
    23761 cacactgagc accacgcgac gcgccagcag cgcaaagccc gccgccacaa tcaccagtcc
    23821 ccacagctct tgttttaact ccagcgccgt cagaaagacg atatacagcc ccagacaact
    23881 ccacaccagc cgcggtttcc actccggcgt ttgcaccccc gtatggtatt gcagcgcctt
    23941 tccagggaaa caacaccagc acaacagcag gagtgtcagc atcattgcgc cagccagcgg
    24001 tgccatttgg gcaataaatc cggcaaacga aagaccagaa cgtccccaga taagaatatt
    24061 ttgcgggttg ccaattggcg tcagtagcga acccgcattg actgccagcg cctcaaaaat
    24121 aatcagccga ttaaccggga tctcacacag tctttttagc gtgatagtca gcggaacaac
    24181 tataaacagc gcaacatcgt tggtcagaaa ggtagaaagc agcgctgccg ccagtaccat
    24241 aaacatcgcc agccgacgct ccgtagcaaa acggcgcacc attttgcgcc ccagcacatc
    24301 aaaataaccg cttaactcga cacctttggt cagcagcatc aggccgctta aggtgatgat
    24361 ggtgtgccag tcgatagcag caggccagga tttcggtgca aagggaacga aaaaacttaa
    24421 tccgatgcca acaagaatta ataactgaaa aaaacgatcg ccttgcagcg tgcgtaaaaa
    24481 aggcaggctc atttggcacc gtgtttctgg gtaaacaaac gaaaggcatc cagcgtctct
    24541 tcactaacat ggtgctccat accttccgcg tcgcgacggg cgatttccgg actgacgccc
    24601 aacaccaaca agaaattttc gactatctga tgtcgctcgc ggctttcctg cgccagcttc
    24661 tctccttctg ccgttaaaaa cacgccacgc caggggatca tttcaatcag ccccatggtt
    24721 gccagtcgct taagcatttt agccaccgtc ggttgcgaaa cccccagacg agcagccatg
    24781 tccacctgac gagcttcccc cacttccctg atcaagtcag aaatcagctc aacgtaatca
    24841 tcaatcagct cgcgccgatg cgcctctctg acctggcgga acccttcaac gtgctcttcc
    24901 acgttcacta actgcgtcac tttttttgct gttggcgtac ctgcgcgacg actcattgtg
    24961 cttcctcatt atggtgacgc atacatagcg ttctcatttc gtgcgcacat tgtaaaccag
    25021 agttgcgaag gtacaaaaaa ttaacgtttt agcaatagct atataatata gcctgtgcta
    25081 tatctgtatg taatgcaatc atccctcaag gatcgatggg attagcaagt caggaggtct
    25141 tatgaatgag ttcaagaggt gtatgcgcgt gtttagtcat tctcccttta aagtacggtt
    25201 aatgctgctc tctatgttgt gcgatatggt caacaacaaa ccgcagcagg ataaaccttc
    25261 cgataaatag cggcgtcgcg gtacgccgct tcactcctgc ttttatgcag gcacaacgcg
    25321 ttttggtctg aaaaacccca ctttttgtcg gatttgcaat ccccttcgca aaagatttgt
    25381 tcgtcggtag ttgacctgaa cagcagctcg ctctatcttc ttgcagccct gcgtatattg
    25441 cggctcgcgg atgcggaccc ctttcccctc ttcacgcact cttgcaggta ttgacccttg
    25501 acgccagggt aagcacatgg cgtttgttac gatagtggca tatgaattta accctcaaag
    25561 aatcgcttgt tacccgtagc cgggtattta gcccgtggac tgcgttctac tttttacagt
    25621 cgctattaat taacctcggc ttaggttacc ccttcagttt gctctacacc gctgcgttta
    25681 cggctatttt gcttttgcta tggcgaacat tgcctcgcgt acaaaaagtt ctggtcggta
    25741 tcagttcgct ggtggcggct tgttatttcc cttttgctca ggcctacggc gcgcctaact
    25801 tcaatacatt gctggcattg cactccacca atatggaaga gtcgaccgaa atcctgacga
    25861 tttttccgtg gtacagctac ctggtcggca tatttatttt tgcgctcggc gtaatagcaa
    25921 tcaggcgaaa aaaagagagt gaaaaaccgc gctggaatac cttcgacagc ctgtgcctgg
    25981 tattcagtgt ggcgacattt tttgttgctc ccgtgcaaaa cctggcctgg ggcggcgtat
    26041 ttaaactgaa agatactggc tatccggtat ttcgttttgc taaggatgtc atcgtcaata
    26101 ataacgaggt gattgaagag caagaacgga tggcaaaact ttccggaatg aaagatacct
    26161 ggacggtcac tgccgttaag ccgaagtatc agacctatgt ggtggtgatc ggagaaagcg
    26221 cgcgtcgcga tgccctcggt gcctttggcg gtcactggga caataccccg tttgccagca
    26281 gcgttaacgg tttgatattt gctgactaca ttgccgccag tggctccacg cagaaatcgc
    26341 ttggcttaac gctcaatcgc gttgtcgatg gcaaaccaca gtttcaggat aactttgtca
    26401 ccctggcaaa tcgcgcgggc ttccagacct ggtggttttc caaccagggc caaatcggcg
    26461 aatacgatac cgccatcgcc agcatcgcca aacgagcaga tgaagtgtac ttcctgaaag
    26521 aaggtaattt tgaagcagat aaaaacacga aagacgaagc gttactggat atgaccgctc
    26581 aagtgctggc gcaagagcac tcgcaaccgc agctgattgt tctacatctg atgggctcgc
    26641 atccgcaggc ctgcgaccgg acacaaggaa aatacgaaac ctttgtgcaa tcgaaagaaa
    26701 cgtcgtgcta tctctatacc atgacgcaaa cagacgattt actgcgcaag ctgtacgatc
    26761 agttacgcaa cagcggcagc agcttctcgc tggtttactt ttctgaccac ggtctggcct
    26821 ttaaagagcg cggcaaagac gtgcaatacc ttgctcatga tgataagtat cagcaaaatt
    26881 tccaggtgcc ttttatggtc atttccagcg acgataaagc gcatcgggtg attaaagccc
    26941 gccgctcggc caatgacttc ttaggctttt tctcgcagtg gacgggaatt aaagcgaagg
    27001 aaataaatat caaatacccg tttatatctg aaaagaaagc cgggccgata tacatcacca
    27061 acttccagtt acagaaggtg gattacaacc atctcggaac cgatattttc gacccgaaac
    27121 cttaaaacaa aaaaatccgc cccgagaggc ggatttttta tatcaccgaa gtgattagaa
    27181 gcggtaacca acaccggcaa tccaagtgcc tacgtcaacg ctacgaatac ggctctgctc
    27241 gtaagagaag tccagagcaa cgttttccat cgggttgaac tgcagaccag caccgtagga
    27301 gaaaccgtag tcgctggtgt cgtgtttgta ggtcgggtat tcagtggtct ggaatttacc
    27361 ataacccaca cccactacac cgtagatgct tgcccagtcg ttaatgcggt aagccggacc
    27421 agcagtgatg ccgtagtact ggtttttgtt gtagtcacca gagcttgcag tacggctttt
    27481 ctcggtgtaa gtgaaagaac cgatcacacc cagcgggctg ttgtcttctt catagcggta
    27541 tttcaggttg aaaccgccca ttttgttcat ttggccctga gcgtcgctct gtgcgtaacc
    27601 gccagttaca gtagaagtcg cagctacgga agtacctgcg gtgaaagcca gaactgcggc
    27661 cagtgctgaa agacatgcaa tttttttcat aaccacctca aatgtgattc aaataagtcc
    27721 taagttttaa atatatcaaa aattaatggg aaactcttcg cgatttatga tgtctaacgg
    27781 accatttcat gtaacagaac gtttccatac accgctatcc atctaaattt aaatcacttt
    27841 ttcagagaac tgcgtaagta ttacgcatgt tttccctgtc attcatccag attattccta
    27901 atcaccagac taatgattcc atcaatcctg gcgcatttta gtcaaaacgg gggaaaattt
    27961 tttcaacaaa tgctcaacca gcattgggta tatccagtac actccacgct ttacttaggt
    28021 ttagatattt gtgggagaaa ggatgcctgg ttcattacgt aaaatgccgg tctggttacc
    28081 aatagtcata ttgctcgttg ccatggcgtc tattcagggt ggagcctcgt tagctaagtc
    28141 actttttcct ctggtgggcg caccgggggt cactgcgctg cgtctggcat taggcacgct
    28201 gatcctcatc gcgttcttta agccatggcg actgcgcttt gccaaagagc aacgattacc
    28261 gctgttgttt tacggcgttt cgctgggtgg aatgaattat cttttttatc tttctattca
    28321 gacggtaccg ctgggtattg cggtggcgct ggagttcacc ggaccactgg cggtggcact
    28381 gttctcttct cgtcgcccgg tagatttcgt ctgggttgtg ctggcggttc ttggtctgtg
    28441 gttcctgcta ccgctggggc aagacgtttc ccatgtcgat ttaaccggct gtgcgctggc
    28501 actgggggcc ggggcttgtt gggctattta cattttaagt gggcaacgtg ctggagcgga
    28561 acatggccct gcgacggtgg caattggttc gttgattgca gcgttaattt tcgtgccaat
    28621 tggaacgctt caggctggtg aagcactctg gcactggtcg gttattccat tgggtctggc
    28681 tgtcgctatt ctctcgaccg ctctgcctta ttcgctggaa atgattgccc tcacccgttt
    28741 gccaacacgc acatttggta cgctaatgag catggaaccg gcgctggctg ccgtttccgg
    28801 gatgattttc ctcggagaaa cactgacacc catacagctg ctggcgctcg gcgctatcat
    28861 cgccgcttca atgggttcta cgctgacagt acgcaaagag agcaaaataa aagaattaga
    28921 cataaattaa atttacattt ct
//
LOCUS       DADSYM010000051        28862 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2415
            SAMN08039579-rid11723173.denovo.051, whole genome shotgun sequence.
ACCESSION   DADSYM010000051 DADSYM010000000
VERSION     DADSYM010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039579
            Sequence Read Archive: SRR6362533
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 28862)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28862)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980162.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 03:39:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,484
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 4,347
            CDSs (with protein)               :: 4,347
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 43 of 85
            Pseudo Genes (incomplete)         :: 37 of 85
            Pseudo Genes (internal stop)      :: 19 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28862
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039579-rid11723173.denovo.051"
                     /strain="LGE2415"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8302 N 95.9628 W"
                     /collection_date="2015-08-24"
     gene            <1..4575
                     /locus_tag="J1Y83_001867"
     CDS             <1..4575
                     /locus_tag="J1Y83_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001271496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HBA7630017.1"
                     /translation="NAVVNQDGELDVSGGGHGIDITGDSATVDNKGGMTVADADSIGI
                     QIDGDKAVVNNDGDNAISNGGTGTQVNGDEATVNNNGSTTVDGKDSTGTEIAGNNATV
                     TQEGELTVSSGGRGIDITGNNAKVDTKGKMTITGTDSVGVSINGDSATLTNTGDIDVS
                     NSATGFSLVTNEGIISLAGSMKVGDFSTGMALSGDNNSVTLAAKDINVTGQKATGVNI
                     SGESNTVDITGNILVDKDQTADNAVDYFYDPSVGVNISGNSNTVSLDGKLTVIADSEL
                     TSRKYMEFDGSQENISGLTVSGDGNTVNLNGGIQFVGEKNALADGSTIADKRSYFGKT
                     PLVSVDGQSKVYLNGDSTISGSLPLGYANILQLSNKAALEIGSDATFSMQDISVYEHY
                     FTQTPQIIKVDTGSQVVNNGDVDIWNISFAGIWGENSTGINNGNITLSQYDYSSPETS
                     FSEPDHMAFLSSSGGSAVNNGTITAKVMEQHSVLNMGSAAGVADPRVFNNSVSSMMGM
                     EAYGKGTVLNSESGVIDMYGRGNIGMLAVDDSAADNAGKITLDTLWVDQNDTTTLRTD
                     LPSSTAIDYGVGMATGTNSGGGARSNGVATNQQGGVITVYNAGAAMAAYGASNMVINQ
                     GIINLEKNGNYDGGLGANMLVGMAVYNRGTAINDKTGVININVDTGQAFYNDGTGTIL
                     NYGEINLLGSPMDSADSHMGAIPENLDLLTALTGSGETDMRTASSGGFVTTKALANYG
                     NETLNSNVAAKAWLYNQDKANLTINGELSIGQGLENSGLLDSDTISAAANVYNRASGS
                     IITDQLSLTGSNSFFNEGNFSGSVAGSSYKQNVVNTGTMAVMADGKSLISGSFLLYNE
                     AGATLSNSSSAVSGGENAIVNVTRTGDSLAQVNRGTITAVNGYSAIKTASTGSNSNGK
                     WIWNTDTGVISGVNPNAPLIDLGRGYNFANAGTINVQGDGAVAISGGTTSYTVQLVNS
                     GTINVGTAQGKADGTNGTGLIGIKGNGSDTTINNAQSGVINVYADNSWAFGGKTKAII
                     NNGEINLLCDTGCDIYAPGTTGTLNDHNSTTDIIVPAATSTPTQGSVPTVPADSSAQQ
                     KLTNYTIGTNSDGTSGMLKANNLVISDNVKVNTGFSAGTADTTVVINDVFKGENISGA
                     ENISSSTVMWNAQGSTDASGNVDVTMTKNAYTDVVTDSSVNNVAQVLDSGYTNNDLYT
                     SLNVGTTAELNSALKQISGSQATTVFNEARVLSNRFSMLSDAAPEVANGLAFNVVAKG
                     DPRAELGNDTQYDMMALRKSLTLTEHQNLSLEYGIARLEGNSSDTAGDNGVTGGYSQF
                     FGLKHQMAFDNGMSWNNALRYDVHNLDSSRSIAYGDVNKTADANVKQQYLEFRSEGAK
                     TFELREGLNVTPYAGVKLRHTLENGYQERNAGDFNLSMNSGSETAVDSIVGLKLDYAG
                     KEGWSANATLEGGPNLSYVKSQRTASISGAGSQRFNIDDGQSGGGFNSLATMGVKYSS
                     QESALQLDAFHWKEDGISDKGVMLNFKKTF"
     gene            4793..5653
                     /gene="pdxI"
                     /locus_tag="J1Y83_001868"
     CDS             4793..5653
                     /gene="pdxI"
                     /locus_tag="J1Y83_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 4-dehydrogenase"
                     /protein_id="HBA7630018.1"
                     /translation="MSSNTFTLGTKSVNRLGYGAMQLAGPGVFGPPRDRHVAITVLRE
                     ALALGVNHIDTSDFYGPHVTNQIIREALYPYSDDLTIVTKIGARRGEDASWLPAFSPA
                     ELQKAVHDNLRNLGLDVLDVVNLRVMMGDGHGPTEGSIEASLTVLAEMQQQGLVKHIG
                     LSNVTPTQVAEARKIAEIVCVQNEYNIAHRADDAMIDALARDGIAYVPFFPLGGFTPL
                     QSSTLSDVATSLGATPMQVALAWLLQRSPNILLIPGTSSVAHLRENMAAEKLHLSEKV
                     LSTLDGISRE"
     gene            5952..6557
                     /locus_tag="J1Y83_001869"
     CDS             5952..6557
                     /locus_tag="J1Y83_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310037.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HBA7630019.1"
                     /translation="MTLYQIKPLFQSLLRPTMFWLYKHHVTANHITLAALALSLLTGL
                     LLVLVAQPILFLLLPIVLFIRMTLNALDGMLARECNQKTRLGAILNETGDVISDIVLY
                     LPFLFLPESNASLVILMLFCTILTEFCGLLAQTINGIRSYAGPFGKSDRALIFGLWGL
                     AVAIYPQWMQWNNLLWSIASILLLWTAINRCRSVLFMSAER"
     gene            6557..7453
                     /locus_tag="J1Y83_001870"
     CDS             6557..7453
                     /locus_tag="J1Y83_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HBA7630020.1"
                     /translation="MLEKSLATLFALLILATLINRFLVWRLPERKGGEVTLRIRTWWG
                     IVICFSMVISGPRWMTLTFFALISFLALKEYCTLISVHFPRWLYWGIPLNYLLIGFNC
                     FELFLLFIPLAGFLILATWRVLVGDPSGFLHTVSAIFCGWIMTVFTLSHAAWLLMLPT
                     INIQGGALLVLFLLALTESNDIAQYLWGKSCGRRKVVPKVSPGKTLEGLMGGVITTMI
                     ASLIIGPLLTPLDTLQALLAGLLIGISGFCGDVVMSAIKRDIGVKDSGKLLPGHGGLL
                     DRIDSLIFTAPVFFYFIRYCCY"
     gene            7469..9226
                     /locus_tag="J1Y83_001871"
     CDS             7469..9226
                     /locus_tag="J1Y83_001871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional alpha/beta hydrolase/class I
                     SAM-dependent methyltransferase"
                     /protein_id="HBA7630021.1"
                     /translation="MENSRIPGEHFFTTSDNTALFYRHWPTLQPGVKKVIVLFHRGHE
                     HSGRLQHIVDELAMPDTAFYAWDARGHGQTSGPRGYSPSLARSVQDVDEFVRFAASDS
                     QVGLEEVVVIAQSVGAVMVATWVHDYAPAIRGLVLASPAFKVKLYVPLARPGLALWHR
                     LRGLFFINSYVKGRYLTHDRQRVASFNNDPLITRAIAVNILLDLYKTSERIVSDAAAI
                     TLPTQLLISGDDYVVHRQPQIDFYQRLRSPLKELHLLPGFYHDTLGEENRAQAFEKMQ
                     SFISRLYANKSQKFDYQHEDRTGPSADRWRLLSGGPVPLSPVDLAYRFMRKAMKLFGA
                     HSAGLHLGMSTGFDSGSSLDYVYQNQPQGSNAFGRLIDKIYLNSVGWRGIRQRKTHLQ
                     MLIKQAVADLHAKGLAIRVVDIAAGHGRYVLDALANEPAISEILLRDYSEVNVAQGQE
                     MIAQRGMSGRARFEQGDAFNPAELSTLTPRPTLAIVSGLYELFPENEQVKNSLAGLAN
                     AIDPGGILIYTGQPWHPQLELIAGVLTSHKDGKPWVMRVRSQGEMDSLVHDAGFDKCT
                     QRIDEWGIFTVSMAVRRDN"
     gene            9216..10532
                     /locus_tag="J1Y83_001872"
     CDS             9216..10532
                     /locus_tag="J1Y83_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310040.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2/dual specificity phosphatase
                     family protein"
                     /protein_id="HBA7630022.1"
                     /translation="MITERRNVLLQGAGWLLLLAPFFFFTYGSLNQFTTTQDLNNHDI
                     PSQVFGWETAIPFLPWTIVPYWSLDLLYGFSLFVCSSTFEQRRLVHRLILATVMACCG
                     FLLYPLKFSFIRPEVSGVTGWLFSQLELFDLPYNQSPSLHIILCWLLWRHFRQHLAVR
                     WRKVCGGWFLLIAISTLTTWQHHFIDVITGLAVGMLIDWIVPVDRRWNYQKPDQRRIK
                     IALPYVVGACSCIVLMELMIMLQLWWSVWLCWPVLSLFIIGRGYGGLGAITTGKDSQG
                     KLPPAVYWLTLPWRIGMWLSMRWFCLRLEPVSKITAGVYLGAFPRHIPAQNAVLDVTF
                     EFPRGRATKDRLYFCVPMLDLVVPEEGELRQAVAMLETLREEQGSVLVHCALGLSRSA
                     LVVAAWLLCYGHCKTVDEAISYIRARRSRIVLKEEHKAMLKLWENR"
     gene            complement(10586..11191)
                     /gene="azoR"
                     /locus_tag="J1Y83_001873"
     CDS             complement(10586..11191)
                     /gene="azoR"
                     /locus_tag="J1Y83_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000048941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-dependent NADH-azoreductase"
                     /protein_id="HBA7630023.1"
                     /translation="MSKVLVLKSSILAGYSQSNQLSDYFVEQWREKHSADEITVRDLA
                     ANPIPVLDGELVGALRPSDAPLTPRQQEALALSDELIAELKAHDVIVIAAPMYNFNIS
                     TQLKNYFDLVARAGVTFRYTEKGPEGLVTGKKAIVITSRGGIHKDGPTDLVTPYLSTF
                     LGFIGITDVKFVFAEGIAYGPEMAAKAQSDAKAAIDSIVAE"
     gene            11392..15294
                     /gene="hrpA"
                     /locus_tag="J1Y83_001874"
     CDS             11392..15294
                     /gene="hrpA"
                     /locus_tag="J1Y83_001874"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase HrpA"
                     /protein_id="HBA7630024.1"
                     /translation="MTEQQKLTFADLQQRLDSLMLRDRQRFLRRLHGVKKVKNPDAQQ
                     AIFQEMAKEIDHAAGKVLLREAARPEITYPDNLPVSQKKQDILEAIRDHQVVIVAGET
                     GSGKTTQLPKICMELGRGIKGLIGHTQPRRLAARTVANRIAEELKTEPGGCIGYKVRF
                     SDHVSDNTMVKLMTDGILLAEIQQDRLLMQYDTIIIDEAHERSLNIDFLLGYLKELLP
                     RRPDLKIIITSATIDPERFSRHFNNAPIIEVSGRTYPVEVRYRPIVEEADDTERDQLQ
                     AIFDAVDELSQESPGDILIFMSGEREIRDTADALNKLNLRHTEILPLYARLSNSEQNR
                     VFQSHSGRRIVLATNVAETSLTVPGIKYVIDPGTARISRYSYRTKVQRLPIEPISQAS
                     ANQRKGRCGRVSEGICIRLYSEDDFLSRPEFTDPEILRTNLASVILQMTALGLGDIAA
                     FPFVEAPDKRNIQDGVRLLEELGAITTDEQASAYKLTPLGRQLSQLPVDPRLARMVLE
                     AQKHGCVREAMIITSALSIQDPRERPMDKQQASDEKHRRFHDKESDFLAFVNLWNYLG
                     EQQKALSSNAFRRLCRTDYLNYLRVREWQDIYTQLRQVVKELGIPVNSEPADYREIHI
                     ALLTGLLSHIGMKDADKQEYTGARNARFSIFPGSGLFKKPPKWVMVAELVETSRLWGR
                     IAARIDPEWVEPVAQHLIKRTYSEPHWERAQGAVMATEKVTVYGLPIVAARKVNYSQI
                     DPALCRELFIRHALVEGDWQTRHAFFRENLKLRAEVEELEHKTRRRDILVDDETLFEF
                     YDQRISHDVISARHFDSWWKKVSRETPDLLNFEKSMLIKEGAEKISKLDYPNFWHQGN
                     LKLRLSYQFEPGADADGVTVHIPLPLLNQVEESGFEWQIPGLRRELIIALIKSLPKPV
                     RRNFVPAPNYAEAFLGRVTPLELPLLDSLERELRRMTGVTVDREDWHWDQVPDHLKIT
                     FRVVDDKNKKLKERRSLQDLKDALKGKVQETLSAVADDGIEQSGLHIWSFGQLPESYE
                     QKRGNYKVKAWPALVDERDSVAIKLFDNPLEQKQAMWNGLRRLLLLNIPSPIKYLHEK
                     LPNKAKLGLYFNPYGKVLELIDDCISCGVDQLIDANGGPVWTEEGFAALHEKVRAELN
                     DTVVDIAKQVEQILTAVFNINKRLKGRVDMTMALGLSDIKAQMGGLVYRGFVTGNGFK
                     RLGDTLRYLQAIEKRLEKLAVDPHRDRAQMLKVENVQQAWQQWINKLPPARREDEDVK
                     EVRWMIEELRVSYFAQQFGTPYPISDKRILQAMEQISG"
     gene            15567..16367
                     /locus_tag="J1Y83_001875"
     CDS             15567..16367
                     /locus_tag="J1Y83_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HBA7630025.1"
                     /translation="MNITPFPTLSPATIDAINVIGQWLAQDDFSGEVPYQADCVILAG
                     NAVMPTIDAACKIARDQQIPLLISGGIGHSTTFLYSAIAQHPHYNTIRTTGRAEATIL
                     ADIAHQFWHIPHEKIWIEDQSTNCGENARFSIALLNQAVERVHTAIVVQDPTMQRRTM
                     ATFRRITGDNPDAPRWLSYPGFVPQLGNNADSVIFVNPLQGLWPVERYLSLLTGELPR
                     LRDDSDGYGPRGRDFIVHVDFPAEVIQAWQTLKHDAVLIEAMESRSLR"
     gene            16564..18003
                     /gene="aldA"
                     /locus_tag="J1Y83_001876"
     CDS             16564..18003
                     /gene="aldA"
                     /locus_tag="J1Y83_001876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HBA7630026.1"
                     /translation="MSVPVQHPMYIDGQFVTWRGDAWIDVVNPATEAVISRIPDGQAE
                     DARKAIDAAERAQPEWEALPAIERASWLRKISAGIRERASEISALIVEEGGKIQQLAE
                     VEVAFTADYIDYMAEWARRYEGEIIQSDRPGENILLFKRALGVTTGILPWNFPFFLIA
                     RKMAPALLTGNTIVIKPSEFTPNNAIAFAKIVDEIGLPRGVFNLVLGRGETLGQELAG
                     NPKVAMVSMTGSVSAGEKIMATAAKNITKVCLELGGKAPAIVMDDADLELAVKAIVDS
                     RVINSGQVCNCAERVYVQKSIYDQFVNRLGEAMQAVQFGNPAERNDIAMGPLINAAAL
                     ERVEQKVARAVEEGARVALGGKAVEGKGYYYPPTLLLDVRQEMSIMHEETFGPVLPVV
                     AFDTLEEAISMANDSDYGLTSSIYTQNLNVAMKAIKGLKFGETYINRENFEAMQGFHA
                     GWRKSGIGGADGKHGLHEYLQTQVVYLQS"
     gene            complement(18045..19046)
                     /gene="gap"
                     /locus_tag="J1Y83_001877"
     CDS             complement(18045..19046)
                     /gene="gap"
                     /locus_tag="J1Y83_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HBA7630027.1"
                     /translation="MSKVGINGFGRIGRLVLRRLLEVKSNIDVVAINDLTSPKILAYL
                     LKHDSNYGPFPWSVDYTEDSLIVDGKSIAVYAEKEAKNIPWKAKGAEIIVECTGFYTS
                     AEKSQAHLDAGAKKVLISAPAGEMKTIVYNVNDDTLDGNDTIVSVASCTTNCLAPMAK
                     ALHDSFGIEVGTMTTIHAYTGTQSLVDGPRGKDLRASRAAAENIIPHTTGAAKAIGLV
                     IPELSGKLKGHAQRVPVKTGSVTELVSILGKKVTAEEVNNALKKATNNNESFGYTDEE
                     IVSSDIIGSHFGSVFDATQTEITAVGDLQLVKTVAWYDNEYGFVTQLIRTLEKFAKL"
     gene            19235..19765
                     /gene="cybB"
                     /locus_tag="J1Y83_001878"
     CDS             19235..19765
                     /gene="cybB"
                     /locus_tag="J1Y83_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415935.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b561"
                     /protein_id="HBA7630028.1"
                     /translation="MGNKYSRLQISIHWLVFLLVIVAYCAMEFRGFFPRSDRPLINMI
                     HVSCGISILVLMVVRLLLRLKYPTPPIIPKPKPMMTGLAHLGHLVIYLLFIALPVIGL
                     VMMYNRGNPWFAFGLTMPYASEANFERVDSLKSWHETLANLGYFVIGLHAAAALAHHY
                     FWKDNTLLRMMPRKRS"
     gene            20010..20183
                     /locus_tag="J1Y83_001879"
     CDS             20010..20183
                     /locus_tag="J1Y83_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA7630029.1"
                     /translation="MKKLALILVMGTLVSFYADAGRKPCSGSKGGISHCTAGGKFVCN
                     DGSISASKKTCTN"
     gene            complement(20255..20404)
                     /gene="hokB"
                     /locus_tag="J1Y83_001880"
     CDS             complement(20255..20404)
                     /gene="hokB"
                     /locus_tag="J1Y83_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokB"
                     /protein_id="HBA7630030.1"
                     /translation="MKRNPLVVCLLIICITILTFTLLTRQTLYELRFRDGDKEVAALM
                     ACTSR"
     gene            20794..22434
                     /gene="trg"
                     /locus_tag="J1Y83_001881"
     CDS             20794..22434
                     /gene="trg"
                     /locus_tag="J1Y83_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein Trg"
                     /protein_id="HBA7630031.1"
                     /translation="MNTTPSQRLGFLHHIRLVPLFACILGGILVLFALSSALAGYFLW
                     QADRDQRDVTAEIEIRTGLANSSDFLRSARINMIQAGAASRIAEMEAMKRNIAQAESE
                     IKQSQQGYRAYQNRSVKTPADEALDTELNQRFQAYITGMQPMLKYAKNGMFEAIINHE
                     SEQIRPLDNAYTDILNKAVKIRSTRANQLAEQAHQRTRLGGMFMIGAFVLALVMTLIT
                     FMVLRRIVIRPLQHAAQRIEKIASGDLTMNDEPAGRNEIGRLSRHLQQMQHSLGMTVG
                     TVRQGAEEIYRGTSEISAGNADLSSRTEEQAAAIEQTAASMEQLTATVKQNADNAHHA
                     SKLAQEASIKASDGGQMVSGVVKTMGAISTSSKKISEITAVINSIAFQTNILALNAAV
                     EAARAGEQGRGFAVVASEVRTLASRSAQAAKEIEGLISESVRLIDLGSDEVATAGKTM
                     CTIVDAVASVTHIMQEIATASDEQSRGITQVSQAISEMDKVTQQNASLVEEASTAAVS
                     LEEQAARLTEAVDVFRLNKHSVSAEPRGAGEPVSFATV"
     gene            complement(22472..23395)
                     /locus_tag="J1Y83_001882"
     CDS             complement(22472..23395)
                     /locus_tag="J1Y83_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBA7630032.1"
                     /translation="MEKNSLFSQRIRLRHLHTFVAVAQQGTLGRAAETLNLSQPALSK
                     TLNELEQLTGARLFERGRQGAQLTLPGEQFLTHAVRVLDAINTAGQSLHRKEGLNNDV
                     VRVGALPTAALGILPSVIGQFHQQQKETTLQVATMSNPMILAGLKTGEIDIGIGRMSD
                     PELMNGLNYELLFLESLKLVVRPNHPLLQENVTLSRVLEWPVVVSPEGTAPRQHSDAL
                     VQSQGCKIPSGCIETLSASLSRQLTVEYDYVWFVPSGAVKDDLRHATLVALPVPGHGA
                     GEPIGILTRVDATFSSGCQLMINAIRKSMPF"
     gene            23612..24955
                     /locus_tag="J1Y83_001883"
     CDS             23612..24955
                     /locus_tag="J1Y83_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HBA7630033.1"
                     /translation="MANSITADEIREQFSQAMSAMYQQEVPQYGTLLELVADVNLAVL
                     ENNPQLHEKMVNADELARLNVERHGAIRVGTAQELATLRRMFAIMGMYPVSYYDLSQA
                     GVPVHSTAFRPIDDASLARNPFRVFTSLLRLELIENEILRQKAAEILRQRDIFTPRCR
                     QLLEEYEQQGGFNETQAQEFVQEALETFRWHQSATVDEETYRALHNEHRLIADVVCFP
                     GCHINHLTPRTLDIDRVQSMMPECGIEPKVLIEGPPRREVPILLRQTSFKALEETVLF
                     AGQKQGTHTARFGEIEQRGVALTPKGRQLYDDLLRNAGTGQDNLTHQMHLQETFRTFP
                     DSEFLMRQQGLGWFRYRLTPSGEAHRQAIHPGDDPQPLIERGWVVAQPITYEDFLPVS
                     AAGIFQSNLGNETRACNHGDASREAFEQALGCPVLDEFQLYQEAEERSKRRCGLL"
     gene            25180..26835
                     /locus_tag="J1Y83_001884"
     CDS             25180..26835
                     /locus_tag="J1Y83_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucan biosynthesis protein"
                     /protein_id="HBA7630034.1"
                     /translation="MDRRRFIKGSMAMAAVCGTSGIASLFSQAAFAADSDIADGQTQR
                     FDFSILQSMAHDLAQTAWRGAPRPLPETLATMTPQAYNSIQYDAEKSLWHNVENRQLD
                     AQFFHMGMGFRRRVRMFSVDPATHLAREIHFRPELFKYNDAGVDTKQLEGQSDLGFAG
                     FRVFKAPELARRDVVSFLGASYFRAVDDTYQYGLSARGLAIDTYTDSKEEFPDFTAFW
                     FDTVKPGATTFTVYALLDSASITGAYKFTIHCEKNQVIMDVENHLYARKDIKQLGIAP
                     MTSMFSCGTNERRMCDTIHPQIHDSDRLSMWRGNGEWICRPLNNPQKLQFNAYTDNNP
                     KGFGLLQLDRDFSHYQDIMGWYNKRPSLWVEPRNKWGKGTIGLMEIPTTGETLDNIVC
                     FWQPEKAVKAGDEFAFQYRLYWSAQPPVHCPLARVMATRTGMGGFPEGWAPGEHYPEK
                     WARRFAVDFVGGDLKAAAPKGIEPVITLSSGEAKQIEILYIEPIDGYRIQFDWYPTSD
                     STDPVDMRMYLRCQGDAISETWLYQYFPPAPDKRQYVDDRVMS"
     gene            26975..27199
                     /locus_tag="J1Y83_001885"
     CDS             26975..27199
                     /locus_tag="J1Y83_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415943.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcH family protein"
                     /protein_id="HBA7630035.1"
                     /translation="MFPEYRDLISRLKNENPRFMSLFDKHNKLDHEIARKEGSDGRGY
                     NAEVVRMKKQKLQLKDEMLKILQQVSVKEV"
     gene            27262..27801
                     /gene="rimL"
                     /locus_tag="J1Y83_001886"
     CDS             27262..27801
                     /gene="rimL"
                     /locus_tag="J1Y83_001886"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12-serine
                     acetyltransferase"
                     /protein_id="HBA7630036.1"
                     /translation="MTETIKVSESLELHAVAESHVTPLYQLICKNKTWLQQSLNWPQF
                     VQSEEDTRKTVQGNVMLHQRGYAKMFMIFKEDELIGVISFNRIEPLNKTAEIGYWLDE
                     SHQGQGIISQALQALIHHYAQSGELRRFVIKCRVDNPQSNQVALRNGFILEGCLKQAE
                     FLNDAYDDVNLYARIIDSQ"
     gene            complement(27793..28773)
                     /locus_tag="J1Y83_001887"
     CDS             complement(27793..28773)
                     /locus_tag="J1Y83_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LbetaH domain-containing protein"
                     /protein_id="HBA7630037.1"
                     /translation="MRKYRLSEEQRAFSYQEDGTKKSVLLRQIIAMSDFNDVIAGSAG
                     GWIDHETVLAQEGNCWIYDQNAIAFGGTVISGNTRITGTSVLWGEVYATDNVWIDNSE
                     ISQGAYISDSVTIRDSLVCGQCRIFGHALIDQHSMIVAAQGLTPDHQLLLQIYDRAKV
                     SASRIVHQAQIYGDAVVRYAFIEHRAEVFDFASVEGNEENNVWLCDCAKVYGHAQVKA
                     GIEEDAIPTIHYSSQVAEYAIVEGNCVLKHHVLIGGNAVVCGGPILLDEHVVIQGESR
                     ITGAVIIENHVELTDHAVVEAFDGDTIHIRGPKVINGEERITRTLLAGLL"
BASE COUNT         7285 a         6726 c         7572 g         7279 t
ORIGIN      
        1 aacgcggtgg tgaaccagga cggcgaactg gatgtgagcg gcggcggtca cgggattgat
       61 atcaccggcg acagcgcaac ggtggataac aaaggtggaa tgaccgtcgc ggatgcggat
      121 tcgattggca ttcagataga cggcgacaag gcggtcgtga acaacgacgg tgacaatgcc
      181 atcagcaacg gcggcaccgg cacgcaggtt aacggcgacg aagccaccgt gaacaataac
      241 ggcagcacca ccgttgacgg caaggattcc accggcaccg agatcgcagg taataacgcc
      301 accgttactc aggaaggtga actcacggtt agcagcggcg gccgcggtat cgacattaca
      361 ggcaacaatg ccaaggtcga cactaaaggc aaaatgacca tcacaggtac tgactcggtt
      421 ggtgtgtcca tcaatggcga cagtgcgacg ttgaccaata cgggcgatat tgacgtcagc
      481 aattcggcta ccggattcag cctcgttacc aatgagggga ttatctcgct ggctggcagc
      541 atgaaagtgg gcgatttttc aacgggcatg gcgcttagcg gcgacaacaa cagcgtgacg
      601 ctggcggcga aagatatcaa tgtcaccggg cagaaagcga caggcgttaa catctcaggc
      661 gagagtaata ccgttgatat caccggtaat attctggtgg ataaagatca aaccgcagat
      721 aacgcggtag attatttcta cgacccatcc gttggtgtga atatcagcgg caacagtaac
      781 acggtatcac tggacggtaa gctaaccgtt attgcagata gtgagttaac ctcacgcaaa
      841 tacatggagt ttgatggtag ccaggagaat atttctgggc ttaccgtgtc tggcgatggt
      901 aacaccgtca atttgaatgg cggcattcag tttgtgggcg agaaaaacgc gctggcagac
      961 ggttcaacta ttgccgataa gcgcagctac tttggtaaga cacctcttgt tagtgtcgat
     1021 ggacaatcca aagtttatct gaacggtgat tctactatca gcggctcttt gccgttaggc
     1081 tatgccaaca ttcttcagct aagcaataag gcggcgcttg aaattggtag tgacgctacg
     1141 ttcagtatgc aggacattag tgtttacgag cactatttca cgcaaactcc gcaaattatc
     1201 aaggttgata cgggttcaca ggtcgtcaac aatggtgatg tggatatctg gaatatcagt
     1261 tttgcaggca tttggggcga aaactctacc ggtattaaca acggaaatat tacgctttct
     1321 caatatgact attcttctcc ggagacatcg ttctcagaac ctgaccacat ggccttttta
     1381 agctccagtg gcggtagtgc tgtgaacaac ggaacgatca ctgcaaaagt gatggaacag
     1441 cacagtgttc tcaatatggg ctctgcagcc ggtgttgcag acccgagggt attcaataat
     1501 agcgtgtcga gtatgatggg aatggaggcc tacggtaaag gcactgttct taacagcgaa
     1561 agcggcgtta ttgacatgta cgggcgcggc aacattggta tgttggcagt tgatgactcc
     1621 gcagcggata acgccgggaa aatcacgctg gatacgcttt gggtcgatca aaacgacacc
     1681 acgacgctgc gtactgacct cccttccagc acggctatag attacggtgt tggtatggcc
     1741 accggaacga atagtggtgg tggcgcaaga agcaacggtg ttgcgactaa ccaacaaggc
     1801 ggtgtaatca ctgtttataa tgccggtgct gctatggctg cttacggtgc cagcaacatg
     1861 gttattaacc agggcattat caatctggag aaaaacggaa attacgatgg cggcttgggc
     1921 gcgaatatgc tcgtggggat ggcggtctat aacagaggca ccgctatcaa cgataaaaca
     1981 ggcgtgatca atattaacgt cgatacgggg caggcgtttt ataacgacgg tacagggaca
     2041 attctgaact atggtgagat aaaccttctc ggctcgccaa tggacagtgc agactctcac
     2101 atgggggcta ttccggagaa tctggatctg ctgacagcac tgacaggcag cggcgaaacg
     2161 gatatgcgaa ctgcttcaag tggcgggttt gtgacgacca aagcgctggc aaactacggc
     2221 aatgaaacgc tgaatagcaa tgtggctgcc aaagcgtggc tttataacca ggataaggcc
     2281 aacctgacaa ttaacggaga gctatccatc gggcaagggc tggaaaacag cggcttactc
     2341 gattctgaca ccattagcgc agccgcgaat gtctataacc gagcatcggg tagcattatc
     2401 actgatcagt tatcgctaac gggtagtaac tcgtttttca atgagggcaa cttcagcggt
     2461 tcggttgctg gctctagcta taagcaaaat gtggttaaca cgggaacgat ggctgtaatg
     2521 gctgacggta agtcgcttat cagtggcagc ttcctgcttt ataacgaagc aggcgcaaca
     2581 ctcagcaaca gcagcagcgc cgtttcaggt ggagaaaatg ccattgttaa cgtaacccgc
     2641 accggcgatt cactcgcgca agttaaccgt ggcaccatta ctgcggtcaa cggctacagt
     2701 gcaattaaaa cggcaagtac gggttctaac agcaacggga aatggatctg gaataccgac
     2761 acgggggtaa ttagcggcgt gaatccaaat gcaccgttaa ttgatcttgg tcgcggctat
     2821 aactttgcca acgccggcac tattaatgtg cagggtgatg gtgcagtcgc tatcagcggg
     2881 ggaaccacca gctatacggt tcaactggtg aacagtggga ccatcaatgt cggtacggcg
     2941 cagggtaaag cggacggcac taacggtact gggttaattg gtatcaaggg gaatggcagt
     3001 gatacgacaa ttaataacgc acaaagcggt gtaattaacg tttatgcaga taattcctgg
     3061 gccttcggtg gaaagaccaa agcgatcatt aacaatggtg aaattaacct gctgtgtgat
     3121 acgggatgtg atatctatgc accgggtacc accggtacgc tgaacgatca taacagtact
     3181 actgatatta tcgttcctgc agcaacgagc acacctacgc agggttcagt ccccacagta
     3241 cctgcggact ccagtgcaca gcaaaaactg actaactaca ctattggtac caatagcgat
     3301 ggcacctctg gtatgttgaa agccaataac ctggtcatca gcgacaacgt caaggtaaat
     3361 accgggttca gtgcaggtac tgccgatacg actgttgtga ttaatgatgt tttcaaaggt
     3421 gaaaatattt ccggggctga gaatatttct tcttccacgg taatgtggaa tgctcagggc
     3481 agcactgatg ccagcggcaa cgtagacgtc acgatgacca aaaacgccta tacggatgtt
     3541 gtgacggatt cttcagttaa taatgtcgct caggtactgg attcgggtta caccaataac
     3601 gacctgtaca ctagtctgaa cgtaggaacg actgctgaac tgaacagtgc gctgaaacaa
     3661 attagcggca gccaggcaac cacggtattc aatgaagcac gagtgttaag taaccgcttc
     3721 agcatgctgt ccgatgcagc accggaagtt gcaaacggtc tggccttcaa cgtggtagcg
     3781 aaaggcgacc cgcgtgctga actgggtaac gacacacagt acgacatgat ggcgctgcgt
     3841 aaatctctga cgctgactga acatcagaac ctgagtctgg aatacgggat tgctcgtctg
     3901 gagggtaata gttcagatac cgcaggcgat aatggtgtga caggcggtta cagccagttc
     3961 ttcggtctga aacaccagat ggcgtttgac aacggtatga gctggaataa cgccctgcgt
     4021 tatgatgtgc ataatctcga tagcagtcgc tcgatagctt acggcgacgt caacaaaacc
     4081 gctgatgcta atgtgaaaca gcagtatctg gaattccgta gtgaaggggc aaaaaccttt
     4141 gaactgcgtg aaggtctgaa tgtgacgcct tatgcgggtg tgaaactgcg tcatacgctg
     4201 gaaaacggtt atcaggagcg taatgcgggc gactttaacc tgagcatgaa cagcggcagc
     4261 gaaacggcgg tggacagcat cgttgggctg aaactggact atgcagggaa agaaggctgg
     4321 agtgcgaacg ccacgctgga aggtgggccg aatctgagct atgtgaaaag tcaacgtact
     4381 gcgagtattt cgggcgcggg aagccagcgt ttcaacattg acgatggtca gagtggcggt
     4441 ggtttcaaca gcctggcgac gatgggtgtg aaatacagta gccaggagag tgcattgcag
     4501 ttagatgcct tccactggaa agaagacggc atcagtgata aaggtgtgat gctaaacttc
     4561 aagaaaacat tctaattttt agcatgtaat acataaaccg caacgctgat acaggttgcg
     4621 gttttttatt gccggatgcg gtgcttgacg tgtcttaacc tgcctggggt ttcgtgtctt
     4681 tcaggacaac agtacaactc atcccgcaat cttgtttaca ccctcgcatt atttatctct
     4741 attcatttct atactgattt ttttaatccg ttgtattaca gggcagggtg cgatgagcag
     4801 caacacattt actctcggta caaaatccgt taaccgtctt ggttatggcg cgatgcaact
     4861 ggcaggtcct ggggtttttg gccccccaag agatcgccac gtcgctataa ccgtgctgcg
     4921 cgaggcgctg gcattgggcg tcaatcatat tgataccagc gacttttatg gtccgcacgt
     4981 caccaatcag attatccgcg aagcgcttta tccgtactct gacgacctga caattgtcac
     5041 taaaattggt gcgcggcgtg gagaggacgc ttcctggtta cccgcatttt ctccggcaga
     5101 gctgcaaaaa gcggtacacg ataatctacg taatctcggc ctggacgtac tggatgtggt
     5161 taacctgcgc gttatgatgg gggatggtca tggcccgacg gaaggatcga ttgaagccag
     5221 ccttaccgtg ctggcggaga tgcaacaaca aggcctggta aaacatattg gcctgagtaa
     5281 cgtcacaccg acgcaggttg cagaggcgcg aaagattgcc gaaatcgtct gcgtgcagaa
     5341 cgaatacaac attgcccacc gtgctgatga cgcaatgatt gatgctttgg ctcgtgatgg
     5401 cattgcctac gtgccgttct ttccgctcgg gggctttacg ccgctgcaat cctccacgct
     5461 ttccgatgtt gctacgagcc tgggtgcaac accaatgcag gtggcgctgg cgtggctgtt
     5521 acagcgttca ccgaatattt tgctgattcc tggaacatct tctgttgcgc atttacggga
     5581 gaatatggct gctgaaaaat tgcatctttc tgagaaagtg ttgtctacgt tggatggtat
     5641 ttcgcgagaa taacgaatat acaaaaggga aagatgcatt tcccttttgt ttctttttta
     5701 atggcatgga gtgcatatgt ttaaggccag gatttgcggc tggataatca gggaaaaggc
     5761 gatggattca ttgattcgta cagtgataga tggtatctca ctgatatcca gacggtaatc
     5821 aaacagatgt gtagggcatt catccaccac atcttgatat attccgaatt tacgttgatg
     5881 ctgcgcgttg tgatatctcc gccgtcggaa ttgatgcacc atagagcaaa ctgaatatca
     5941 cggaatgata aatgacgctc taccagataa aaccgctctt tcagtcgctg ttaaggccga
     6001 cgatgttttg gctttataag caccatgtta cagcgaatca catcactctt gctgcactgg
     6061 cgctttctct tcttaccgga ttgctgttgg tgttggtggc acaacccatc ctctttttgc
     6121 tattgcccat cgtgcttttt atccgtatga cgctcaatgc gctggatggc atgttggcgc
     6181 gtgagtgcaa tcagaaaaca cgtctggggg cgattttgaa tgaaactggc gatgttattt
     6241 ccgatattgt gctctattta ccgtttttat ttttaccgga aagtaacgca tcactcgtaa
     6301 tactcatgtt attttgcacc atattgactg agttttgcgg tttactcgcg cagacgatta
     6361 acggtattcg cagttatgcc ggtccatttg gcaaaagcga tcgcgcgtta atatttggtc
     6421 tgtgggggct agccgttgcc atttatccac agtggatgca atggaataat cttttatgga
     6481 gcattgcttc aattctgctt ctctggactg cgattaatcg ttgtcggagt gtgcttttta
     6541 tgagtgcaga aagataatgc tggaaaaatc tctggcaaca cttttcgcgt tgttaatttt
     6601 agcaacgttg ataaaccgtt ttctggtatg gcggttaccg gagagaaaag ggggtgaggt
     6661 tacattacgt attcgtacct ggtggggcat cgtcatttgt ttttcaatgg tgatttcagg
     6721 tccacgctgg atgacgttga cgttttttgc gctgataagt tttctggcat tgaaagaata
     6781 ttgtacgctt atatctgtac attttccgcg ttggttatat tggggtattc ctcttaacta
     6841 tttgcttatc ggttttaatt gctttgagct gtttctgtta tttatacctt tggctgggtt
     6901 tctgatatta gccacctggc gagttttagt gggtgacccc tccggttttc tgcataccgt
     6961 gagcgccatt ttttgtggct ggataatgac cgttttcacc ttgagtcatg ccgcctggtt
     7021 attaatgttg ccaaccataa atatccaggg cggggcgtta ctggtgttat ttcttttggc
     7081 attaaccgag tcaaacgata ttgcacagta tttatgggga aaatcctgcg gcagaagaaa
     7141 agtggtccct aaagtcagcc cgggaaaaac attggaaggg ctgatgggcg gcgtaatcac
     7201 caccatgatt gcgtcactga ttattggacc gttgcttaca ccgctagata cattacaggc
     7261 attattagcg ggtttgttaa ttggtattag tggtttttgc ggcgatgtgg tgatgtcagc
     7321 catcaaacga gatattggtg ttaaagatag tggaaaacta ttaccaggac atggcggact
     7381 ccttgacagg attgactcat taattttcac cgctccggta tttttttatt ttattcgcta
     7441 ctgctgttac tgaaggaaaa tagagaaaat ggaaaattca cgcatccctg gggaacattt
     7501 ttttaccacc agtgataata cagcgttgtt ttatcggcac tggcccactt tacagccagg
     7561 ggtgaaaaag gtcatcgtct tatttcatcg cggtcatgaa cattctggtc gtctacaaca
     7621 tatcgttgat gaactggcga tgccagatac tgctttttat gcatgggatg cccgagggca
     7681 tggacaaact tcggggccgc gtggttatag tccgtctctt gcgcgttcag tgcaggatgt
     7741 tgatgaattt gtccgttttg ctgccagcga cagccaggtc ggactggaag aggtggttgt
     7801 gatcgcgcaa agcgtcggcg cagtgatggt tgccacatgg gttcatgatt atgcgcctgc
     7861 tattcgcgga ctagtgctgg catctccggc ctttaaggtt aaattgtatg tgccgctggc
     7921 acgtcctggg ctggcgttat ggcatcgtct gcgtggtctg ttttttatta attcctatgt
     7981 gaaagggcgt tatctgaccc acgatcggca acgggtggcg agtttcaata atgatccgct
     8041 gatcacacgg gcgattgccg tcaatatctt gctcgatctc tataaaacgt ctgaacgtat
     8101 tgttagcgat gcggcggcga ttacgctccc cacgcaactt ctgatatcag gcgatgacta
     8161 tgtggtgcat cgccaaccgc agattgattt ttatcagcgt ttacgtagtc ctctgaaaga
     8221 gctgcatctg ctgccaggct tttatcacga cacgttgggt gaagagaaca gggcgcaggc
     8281 atttgaaaaa atgcaaagct ttatcagccg tttatatgct aacaagtcac aaaaatttga
     8341 ttatcagcat gaagatcgca ctggaccatc agcggatcga tggcggctcc tttctggtgg
     8401 acccgtgccg ttatcgccgg ttgatttggc gtatcgcttt atgcgaaagg cgatgaaact
     8461 gttcggggcg cactctgcgg gcctgcatct cggaatgagt accggctttg attcaggcag
     8521 ttcgctggat tatgtctatc aaaatcaacc acaaggcagt aacgcattcg ggcgcttaat
     8581 cgacaaaatc tacctgaaca gtgttggctg gcgcggtatt cgccagcgca aaacccattt
     8641 acaaatgctg attaaacaag ccgttgccga tctccacgct aaaggtttag ccatccgcgt
     8701 ggttgacatt gccgcagggc atgggcgcta tgtactggat gcgctggcaa acgagcccgc
     8761 cataagcgaa attttgttac gtgattacag tgaggtaaat gttgcacaag ggcaagagat
     8821 gattgcccaa cggggaatgt ctgggcgggc gcgttttgaa cagggcgatg cgtttaatcc
     8881 ggcagaactc agcacgttaa ctccgcggcc tacgctggcg attgtctctg gcctgtatga
     8941 gctttttccc gaaaatgagc aggtaaaaaa ctcactcgca ggtcttgcca atgccatcga
     9001 tccgggtggc attctcatct acaccgggca gccgtggcac ccacaactgg agctgattgc
     9061 cggggtgtta accagtcata aagatggtaa accgtgggta atgcgcgtgc gttcgcaagg
     9121 ggagatggat tcgctcgtgc atgatgccgg attcgataaa tgcacacaac ggattgatga
     9181 gtggggcatt tttacggttt cgatggcggt gcgtcgtgat aactgaacgc cggaacgtat
     9241 tgctacaagg agctggctgg ttattgttgc tggccccatt tttcttcttc acctatggat
     9301 ctcttaatca gttcaccacg actcaggatc ttaacaacca tgatatcccc agtcaggtat
     9361 tcggttggga aacggcgatc ccttttcttc cctggactat tgttccttac tggagtctgg
     9421 atcttttata tggattttcg ctgttcgttt gtagctcgac atttgagcag cgccgactgg
     9481 tccaccggct tattctggca acggtaatgg cctgctgcgg ttttttgctc tacccgctga
     9541 agtttagttt tatccgtcct gaagtgagtg gggtgacagg atggctattt tcgcaacttg
     9601 aactgtttga tctgccttat aaccagtctc cttcgctgca tattattctc tgctggctac
     9661 tttggcgtca ctttcgtcag catctggctg tgaggtggcg taaagtctgc ggcggatggt
     9721 ttttactcat cgccatttcg acgctgacga cctggcagca tcattttatt gatgtcatca
     9781 cggggctggc ggtaggtatg ttaattgact ggatagtgcc cgtcgaccgt cgttggaatt
     9841 atcagaaacc tgatcaacgt cgaatcaaaa tagcactgcc atatgtcgta ggcgcgtgct
     9901 cgtgcattgt gttgatggag ctaatgataa tgcttcagtt atggtggtca gtctggttat
     9961 gttggccagt attatcgcta ttcatcattg gccgtgggta cggtgggctt ggcgcgataa
    10021 caacagggaa agatagtcag gggaaactcc cgcccgccgt ttactggctg acattgccct
    10081 ggcgtatcgg gatgtggctg tctatgcgtt ggttttgtct tcgcctggag ccggtgagca
    10141 aaattactgc tggtgtttat ttaggggcgt ttccacgaca tattccggca cagaatgcgg
    10201 ttctggacgt cacctttgaa ttccctcgcg gacgagcgac aaaagatcga ctctattttt
    10261 gtgtaccgat gttggatctg gtggttccgg aagaggggga gctccgacag gccgtggcga
    10321 tgctggaaac attacgcgaa gagcaaggca gcgttctggt ccattgcgcg ttgggattat
    10381 cgcgcagtgc gctggtggtg gcggcatggc tgttatgtta cggacattgt aaaaccgttg
    10441 atgaagcgat tagttatatt cgagccagac gctcgcggat tgtgcttaag gaagagcaca
    10501 aagcgatgct gaaattatgg gaaaacaggt aagcggattg agatgtggac tgaatatcta
    10561 cagtccacat caagatcgtg tccggttatt cagcaacaat actgtcgatg gctgctttcg
    10621 cgtcagactg cgctttcgct gccatttccg ggccgtacgc gatcccttcg gcgaagacaa
    10681 atttcacatc ggtaatgccg ataaagccca ggaatgtgga cagatatggc gttaccagat
    10741 ccgttgggcc atctttatgg atcccaccgc ggctggtgat aacgatggct tttttaccgg
    10801 tgaccaggcc ttccggaccc ttctcggtat agcggaaagt aacgcctgca cgtgcaacca
    10861 ggtcaaaata gtttttcaac tgggtcgaga tattgaagtt atacatcggt gccgcaataa
    10921 cgataacgtc gtgggctttc agctcggcaa tcagctcatc ggaaagcgcc agagcttcct
    10981 gctgacgcgg agtcagcggc gcatcgctcg gacgcagtgc accaaccagt tcgccatcca
    11041 ggaccggaat cggatttgca gccaggtcac gcacggtgat ttcatcagcg gagtgctttt
    11101 cgcgccattg ttcaacaaaa taatcggaca actgattaga ctgagagtac cctgccagga
    11161 tgctggattt aagaactaat accttgctca tggtgtttcc ttatagatat ttgaatgggc
    11221 aatgccccgt tgcttgttga cactttattc acaatcctgc cacagagata gcgcaataaa
    11281 tcgaagccta tgttcgaatt tattgaacaa cgcatagaaa gccgcgatgt ggtactctat
    11341 atctatcatt taaaagaaaa ttaatcaggc agcctactgc ccactaacgt tatgacagaa
    11401 caacaaaaat tgacctttgc tgacttgcag cagcggctgg attcgctgat gttgcgcgac
    11461 agacagcgtt ttttacgccg tctgcacggt gtgaagaagg ttaaaaatcc tgatgcacaa
    11521 caagccattt tccaggagat ggcgaaagag attgaccatg cggcagggaa agtcctgctg
    11581 cgtgaagcgg cacgaccgga gattacttac ccggacaatt tgccggttag tcagaaaaag
    11641 caggacattc tcgaagcgat tcgtgaccac caggtggtga tcgtcgccgg ggaaacgggt
    11701 tccggtaaaa cgactcagct accgaaaatc tgtatggagc tggggcgcgg gattaaaggg
    11761 ctgatcggcc atacccagcc gcgtcggctg gcggcgcgaa cggtggcgaa ccgtattgcg
    11821 gaagagctaa aaacggagcc gggcggttgc atcggttaca aagtgcgttt cagcgatcac
    11881 gtcagtgata acacgatggt caagctgatg accgacggta tcctgctggc agagatccag
    11941 caagaccgcc tgctgatgca gtacgacacc atcattattg acgaagcgca cgaacgcagc
    12001 ctgaatatcg attttctgct cggctatttg aaagagttgc tgccgcggcg tcctgaccta
    12061 aaaattatta tcacttccgc gactatcgac ccggaacgct tttcgcgcca ctttaataat
    12121 gcgccgatta tcgaagtctc cggtcggacc tatccggtgg aagtgcgcta tcgcccgatt
    12181 gttgaagaag ccgatgacac cgagcgcgac cagttgcagg cgatttttga cgccgtagat
    12241 gaactgagcc aggaaagccc tggcgacatt ctgatcttta tgagcggcga gcgagaaatt
    12301 cgcgataccg ccgatgcgct gaacaagctg aacttacgcc ataccgaaat cttgccgctt
    12361 tatgcacggc tttccaacag cgaacagaac cgcgtgttcc agtcgcacag cgggcgacgc
    12421 attgtactgg cgaccaacgt cgcggaaacc tcgctgaccg taccgggtat taaatacgtt
    12481 atcgacccag gtacggcacg tatcagccgc tacagctatc gcaccaaagt gcagcgtttg
    12541 ccgattgagc caatttcgca ggcttcagct aatcagcgta aaggccgctg cggtcgtgtg
    12601 tcagaaggga tctgtattcg tctctattcc gaagacgatt tcctctcgcg cccggagttt
    12661 accgacccag aaattctgcg tactaacctg gcctcggtta ttttgcagat gaccgcactg
    12721 ggcttgggcg atatcgccgc attcccgttt gtcgaagcac cggataaacg caatatccag
    12781 gatggcgtgc gtctgctcga agaactgggc gcgatcacca cggatgaaca ggccagcgcc
    12841 tataaactga cgccgcttgg tcgccagctc tcgcagttgc ctgtcgaccc acgtctggcg
    12901 cgtatggtgc tggaagcgca aaaacatggc tgcgtgcgtg aggcgatgat tatcacatcc
    12961 gcgctctcca ttcaggatcc gcgtgagcgt ccgatggata aacagcaggc atctgacgaa
    13021 aaacatcgtc gcttccacga caaagagtct gacttcctgg catttgtgaa tctgtggaat
    13081 tatcttggcg agcagcaaaa ggcgctttct tcaaacgcct tccgtcgcct gtgtcgtact
    13141 gattatctca actatctgcg cgtgcgcgaa tggcaggata tctacaccca gttgcgccag
    13201 gtggtgaaag aacttggcat tccggttaac agcgaaccgg cggattatcg cgaaattcac
    13261 attgcgttgc tgaccggttt gctttcgcat atcggcatga aagatgccga taaacaagaa
    13321 tataccggcg cacgtaacgc gcgtttctcc atcttccccg gttctggttt attcaaaaag
    13381 ccgccgaaat gggtaatggt ggcggaactg gtagaaacca gccgcctgtg ggggcgcatt
    13441 gcggcacgca tcgatccaga atgggtggaa cctgtcgctc agcatttaat caaacgcacc
    13501 tacagcgaac cgcactggga acgggcgcag ggcgcggtga tggcaacgga aaaagtcact
    13561 gtttatggtt taccgattgt tgctgcgcgt aaggtcaact acagccagat cgatccggcg
    13621 ttatgtcgtg aactctttat tcgccacgcg ctggtggaag gtgactggca gacgcgtcac
    13681 gcattcttcc gtgaaaacct gaaactacgg gccgaagtgg aagaactgga acacaaaact
    13741 cgtcgccgcg atattctggt ggatgacgaa acgctgtttg agttctacga ccagcgcatc
    13801 agccacgatg tgatttccgc acgtcacttc gacagctggt ggaaaaaagt cagccgcgaa
    13861 acgcctgatt tgctcaactt tgaaaagagc atgttgatca aagaaggggc ggaaaaaatc
    13921 agcaagctgg attacccgaa cttctggcat cagggcaatc tcaagctgcg tttgagctat
    13981 cagtttgagc ccggcgcgga tgctgacggt gtgaccgtgc atatcccgct gccgttactt
    14041 aaccaggttg aggagagtgg gtttgagtgg cagatccccg gtctgcgtcg tgagctgatc
    14101 atcgccctga ttaaatcgct gccgaagccg gtacgccgta attttgtgcc cgcgccaaac
    14161 tacgccgaag cgtttttagg ccgcgtcaca ccgctggaat tgccgttgct cgacagcctt
    14221 gaacgagagt tacggcggat gactggcgtc accgttgacc gcgaagactg gcactgggat
    14281 caggtgcccg atcacctgaa aatcactttc cgcgtggtgg atgacaaaaa caagaagcta
    14341 aaagaaaggc gctcattaca ggatctgaaa gacgccctga aaggcaaagt gcaggaaacg
    14401 ctgtctgccg tggcggatga cggtatcgag cagagcggtt tacatatctg gagttttggt
    14461 caactgccgg aaagctacga gcagaagcgc ggcaactaca aagtgaaggc ttggccggcg
    14521 ctggtggatg agcgtgacag cgtagcgatc aaactgtttg ataacccgct ggaacagaag
    14581 caggcaatgt ggaacggtct tcgccgttta ctgctgctaa atattccatc gccgatcaag
    14641 tacttgcacg aaaagctgcc aaacaaagcc aagctggggc tgtactttaa cccgtatggc
    14701 aaagtgctgg agctgatcga tgactgtatc tcctgcggtg tggatcaact gatcgacgcc
    14761 aacggcggtc cggtctggac ggaagaaggc tttgctgcgc tgcatgaaaa agtccgcgct
    14821 gaactgaacg acacagtggt ggatattgcg aagcaggtcg agcaaatcct cactgcggtg
    14881 ttcaatatca acaaacgtct gaaagggcgg gtggatatga ccatggcgct ggggctttct
    14941 gacattaaag cgcagatggg cgggctggtg tatcgcggtt ttgtcactgg taacggcttc
    15001 aaacggctgg gcgacacgct gcgttatttg caggcaattg aaaaacgact ggaaaaactg
    15061 gcggtcgatc cacatcgtga ccgtgctcag atgctgaaag tcgaaaacgt ccagcaggca
    15121 tggcagcaat ggatcaacaa actgccgcct gcacgtcgtg aggatgaaga cgtgaaagag
    15181 gtccgctgga tgatagaaga gctgcgcgtt agttacttcg ctcaacaatt tggtacgcct
    15241 tatccgattt cggataagcg tattttgcag gcgatggagc agattagcgg ttaacactgc
    15301 tatttgcctg ataaatctaa aacccggtaa gcatttagcg ccgggttttt tattaattct
    15361 aaaaactata agggaaaaaa ttcaggagtt cagaaaaata caattctctg ctgcaagatg
    15421 aataatgttt atctacagca tttccttaaa aaatatgtca ggcttgcgga gtggcagtta
    15481 aggaaatacg acttcctcct ttcagagtgc tccgcttctc actattatct cacgcagtat
    15541 tcttaaggga acgataagga ggaaccatga atattacccc gtttccgacg ctttcgccgg
    15601 caaccataga tgccataaat gttatcggac agtggctggc gcaggatgat ttctccggtg
    15661 aggtgccgta tcaggccgat tgcgtgatcc ttgcaggcaa tgcggttatg ccgactatcg
    15721 atgcggcatg taagattgcc cgcgatcagc aaattccttt actgattagt ggtggtatcg
    15781 gtcactcgac aacttttttg tatagcgcca tcgcacagca tccacactac aacactatcc
    15841 gcaccactgg cagagctgaa gctaccatcc tggcggatat cgctcatcag ttctggcata
    15901 ttccgcatga aaaaatctgg attgaagacc agtcaacaaa ctgcggtgaa aacgcacgct
    15961 ttagcatcgc gctattgaat caggccgtag aacgagttca tacggctatc gttgttcagg
    16021 accccaccat gcagcggcgc acgatggcga cgttccgccg tataaccggg gataatcccg
    16081 atgcaccacg ctggttaagt tatcccggat tcgttcctca gttaggaaat aacgcagaca
    16141 gtgtaatctt tgttaatccg ttgcaaggat tatggccggt tgagcgttat ctatcgctac
    16201 tcactggcga gctgccgcgt ttacgcgatg atagcgatgg ctacggcccc cgcgggcgag
    16261 attttatcgt tcacgttgat tttccggcag aagtcatcca ggcatggcaa acgctgaaac
    16321 acgatgcggt gctcatcgag gcgatggaaa gtcgctcgtt acgttaaaaa ttgcccgttt
    16381 gtgaaccact tgtttgcaaa cgggcatgac ttctgacttt tatttctgcc tttaattcct
    16441 tttacacttg tttttatgaa gcccttcaca gaattgtcct ttcacgatgc cgtctctctg
    16501 atgattgata ttatttaaca ataaatttac caaaaacaaa catacaaatc acaggagtcg
    16561 cccatgtcag tacccgttca acatcctatg tatatcgatg gacagtttgt tacctggcgt
    16621 ggagacgcat ggattgatgt ggtaaaccct gctacagagg ctgtcatttc ccgcattccc
    16681 gatggtcagg ccgaggatgc ccgtaaggca atcgatgcag cagaacgtgc acaaccagaa
    16741 tgggaagcgt tgcctgctat tgaacgcgcc agttggttgc gcaaaatctc cgccgggatc
    16801 cgggaacgtg ccagtgaaat cagtgcgctg attgtcgaag aagggggcaa gatccagcag
    16861 ttggctgaag tcgaagtggc ttttactgcg gactatatcg attatatggc ggagtgggca
    16921 cggcgttacg agggcgagat tattcaaagc gatcgtccag gagaaaatat ccttctgttt
    16981 aaacgtgcgc ttggcgtgac taccggcatt ctgccgtgga actttccgtt cttcctcatt
    17041 gcccgcaaaa tggctcccgc tcttttgacc ggtaatacca tcgtcattaa acccagtgaa
    17101 tttacgccaa acaatgcgat tgcattcgcc aaaatcgttg atgaaatagg ccttccgcgc
    17161 ggcgtgttta accttgtact cgggcgtggt gaaacccttg ggcaagaact ggcgggtaac
    17221 ccaaaggtcg caatggtcag tatgacaggc agcgtctctg caggtgaaaa gattatggcg
    17281 actgcggcga aaaatatcac caaagtgtgc ctggaattgg ggggtaaagc accggctatc
    17341 gtaatggacg atgccgatct tgaactggca gtcaaagcca tcgttgattc acgcgtcatt
    17401 aatagtgggc aagtgtgtaa ctgtgcagaa cgtgtttatg tacagaaaag catttatgat
    17461 cagttcgtca atcggctggg tgaagcgatg caggcggttc aatttggtaa ccccgctgaa
    17521 cgcaacgaca ttgcgatggg accgttgatt aatgccgcgg cgctggaaag ggttgagcaa
    17581 aaagtggcgc gcgcagtaga agaaggggcg agagtggcgt tgggtggcaa agcggtagag
    17641 gggaaaggat attattatcc gccgacattg ctgctggatg ttcgccagga aatgtcgatt
    17701 atgcatgagg aaacctttgg cccggttctg ccggtggtcg catttgacac gttggaagag
    17761 gctatctcaa tggctaatga cagtgattac ggcctgacct catcaatcta tacccaaaat
    17821 ctgaacgtcg cgatgaaagc cattaaaggg ctgaagtttg gtgaaactta catcaaccgt
    17881 gaaaacttcg aagctatgca aggcttccac gctggatggc gtaaatccgg tattggcggc
    17941 gcagatggta aacatggctt gcatgaatat ctgcagaccc aggtggttta tttacagtcg
    18001 taatgagtga aagaggcgga ggttttttcc tccgcctgtg cgcgtcagag tttagcgaat
    18061 ttttcgaggg tgcgaataag ctgcgtgacg aagccatatt cgttatcgta ccaggcgacc
    18121 gttttcacca gttgtaaatc gcccacggcg gtaatttccg tttgcgtggc atcaaacacc
    18181 gaaccgaaat ggctgccaat gatatcggaa gagactattt cttcatcagt ataaccaaat
    18241 gactcgttat tgtttgtcgc ctttttaagc gcgttgttca cctcttcggc agtcactttc
    18301 tttccgagaa tcgacaccag ttcagtgacc gaacctgttt tcaccggcac gcgttgcgca
    18361 tgacctttca gtttgccgct cagttccggg atcaccagac caatggcttt tgccgccccc
    18421 gtagtgtggg gaatgatatt ttctgccgct gcgcgtgaag cacgtaaatc tttaccacgt
    18481 gggccatcca ccagtgactg ggtgccggta taggcatgaa tggttgtcat cgtgccgact
    18541 tctatcccga aactgtcatg caaggctttg gccatcggcg caaggcagtt agtggtgcat
    18601 gacgccacgg aaacaatggt gtcgttgcca tccagagtgt cgtcattgac gttataaacg
    18661 atggttttca tttcaccggc aggggcggaa atcaacacct tcttcgcgcc agcatcaaga
    18721 tgcgcctgcg atttctcggc ggaggtataa aagcctgtac attcgacaat gatttctgcg
    18781 cctttcgctt tccacggaat atttttagcc tctttttcgg cgtaaaccgc gatacttttc
    18841 ccatcaacga taagtgaatc ttccgtataa tcaacgctcc aggggaacgg tccgtagttt
    18901 gaatcatgtt tcagcaggta ggcgagaatt tttggggaag tgagatcatt aatagcgaca
    18961 acgtctatgt tgcttttgac ttcaagtaat cgacgcaaca ccagtcgacc gatacgacca
    19021 aaaccattaa taccaacttt actcatggtc ttctcctgtc aggaacgttc ggatgaaaat
    19081 tgatcctttc caggcttaga ccaggatggc gggatgggca atctacattc ttgcagcgaa
    19141 acgtaacgta ctgaaaacgg gtgaacaata tttaatgaaa ttttaagaaa agcccgttat
    19201 gttaacggca aattatgtta aagcaggaaa tgttatggga aataaatact caaggttaca
    19261 aatcagcatt cactggctgg tctttttgct ggttatcgta gcgtattgcg caatggagtt
    19321 tcgtggtttc ttcccacgta gcgatcggcc cctcatcaac atgattcatg tttcctgtgg
    19381 catctcaatt ctcgtactga tggtggttcg tcttttgtta aggctgaaat acccaacccc
    19441 gccgattata cctaagccaa aaccgatgat gacgggactg gcgcatttgg gccatttggt
    19501 gatttatctc ctgtttattg cgctgccggt gattggtttg gtgatgatgt ataaccgggg
    19561 caacccgtgg tttgcgtttg gtttgacgat gccttacgct tcagaggcca atttcgagcg
    19621 ggttgatagc ttaaagtcgt ggcatgaaac gctggcgaat ctgggatatt ttgtcatcgg
    19681 gttgcacgct gcggctgcac tggcacacca ctatttctgg aaggacaaca cacttctacg
    19741 catgatgcca cgtaaacgtt cctgaaggat atttaaagaa aacgcctgta ctaaaaccga
    19801 cccgtggtac aggcgaagaa tacgggccta catcggaagc gcctattata tttatttgta
    19861 tgacaaataa aaccataatc cttgtctata cgtccatctg gcttatcttt aatcaattca
    19921 cccgatcttt gatctcatca acggtatcaa aataaaccag cgtataacgt tcaaatcgaa
    19981 cataagattc gataaatcat ggaggttata tgaaaaaact ggcacttatc ctggttatgg
    20041 gaacgcttgt ttccttttat gccgatgccg ggcgcaaacc ctgttctggt tcgaaagggg
    20101 ggatctcaca ctgtacggca ggcggcaaat ttgtttgtaa tgatggttct attagtgcat
    20161 cgaaaaaaac atgcactaac tgaagtgtaa aaggggtgcc atgagaaaat gaatccgcta
    20221 gtcagatgcg cggggaaaat ccccgcgctt gcccttacct ggacgtgcag gccatgagcg
    20281 cagcaacctc cttatcaccg tcccggaacc gcagttcgta gagcgtttgt cgggtcagga
    20341 gtgtgaatgt cagaatcgta atgcagataa tgagcagaca caccaccaga gggttgcgct
    20401 tcatagcctt tctccttgcc ggatggcggg taagaggcta acatctgaat tgcttggttc
    20461 attcgttggc ctcggttgat agaaatatcg gtcggggcct tcgtctttct gattcccggt
    20521 tagcctgaaa acagaaagtc tcaggcaccc gcaggcatcc tatgaggttt ccttagggac
    20581 gaaaataatc acttgacgaa attgcgtgct gttttccaga atttttcgtc attcggttta
    20641 gccgttcgtt actctcttca ttccaatggc attattcttc tctgccataa ttgttgtaaa
    20701 aatgtgacgc aaagaggttt ttggtcataa gtaattaccg tcaagtgccg atgactttct
    20761 atcaggagta aacctggacg agagacaacg gtaatgaata caactccctc acagcgatta
    20821 ggttttttgc atcacatcag gctggttccg ttatttgcct gcattcttgg cggtatctta
    20881 gttctattcg cattaagttc tgctctggct ggctatttcc tctggcaggc cgatcgcgat
    20941 cagcgtgatg ttactgcgga gattgagatt cggaccgggt tagcgaacag ttcagatttt
    21001 ttgcgttcag cccggatcaa tatgattcag gccggggcag cgagtcgtat tgcggaaatg
    21061 gaagcaatga agcgaaatat tgcgcaagcc gaatcggaga ttaaacagtc gcagcaaggt
    21121 tatcgtgctt atcagaatcg atcggtgaaa acacctgctg atgaagccct cgacactgaa
    21181 ttaaatcaac gcttccaggc ttatatcacg ggtatgcaac ccatgctgaa atatgccaaa
    21241 aatggcatgt ttgaggcgat tatcaatcat gaaagtgagc agatccgacc gctggataat
    21301 gcttataccg atattttgaa caaagccgtt aagatacgta gcaccagagc caaccaactg
    21361 gcggaacagg cccatcagcg cacccgcctg ggtgggatgt tcatgattgg cgcgtttgtg
    21421 cttgccctgg tcatgacgct gataacattt atggtgctac gtcggatcgt cattcgtcca
    21481 ctgcaacatg ccgcacaacg gattgaaaaa attgccagtg gcgatctgac gatgaatgat
    21541 gaaccggcgg gtcgtaatga aatcggtcgc ttaagtcgtc atttacagca aatgcagcat
    21601 tcactgggga tgacagtagg gactgttcga cagggtgcgg aagagattta tcgtggcacc
    21661 agcgaaattt cagctggcaa tgcggacctg tcatctcgca ccgaagaaca agcggcggct
    21721 atcgaacaaa ctgccgccag catggagcaa ctcactgcga cggtgaaaca gaatgcggat
    21781 aacgcgcatc atgccagcaa actggcgcaa gaggcttcta ttaaagccag cgatggcggg
    21841 cagatggttt ccggtgtagt aaaaacgatg ggcgctatct ccacgagttc gaagaaaatt
    21901 tctgagatca ccgccgtcat caacagtatt gctttccaga cgaatattct ggcactgaat
    21961 gctgccgttg aagccgcgcg agcgggtgag caaggacgtg gatttgccgt tgtcgccagc
    22021 gaagtacgga cactcgcaag ccgcagcgct caggcggcga aagagattga aggcttgatc
    22081 agtgaatcag tcaggttaat tgacctggga tcggatgagg tggcaacggc agggaaaacc
    22141 atgtgcacta ttgttgatgc cgtcgcgagt gtcacacata tcatgcagga aatcgccacc
    22201 gcctcggatg aacaaagtcg aggcataacg caggttagcc aggcgatttc tgaaatggat
    22261 aaggtgacgc aacagaatgc ttctctggta gaagaggcct caacggcggc ggtgtccctt
    22321 gaagaacagg cggcacgact aactgaggcg gtggacgtat tccgtctgaa caaacattct
    22381 gtgtcggcag aaccacgagg agctggtgaa ccagttagtt ttgctacggt gtgaaaatgt
    22441 taaaggaggg atcgacagat cccttcacct ttcagaacgg cattgatttt cgaatagcgt
    22501 taatcatcaa ctggcaacca gaagagaacg tcgcatctac gcgggtcagt attccaatcg
    22561 gttcgcctgc accatgtccc ggaacaggca gggctaccag cgtggcatga cgcaggtcgt
    22621 cttttacagc gccagaaggg acaaaccaca cgtaatcgta ttcaaccgta agttgacgag
    22681 atagcgaagc agacagcgtt tcgatacaac ccgaaggaat tttacatccc tggctctgca
    22741 ctaatgcatc tgaatgctgg cgtggcgcag tgccttctgg tgatacaacg accggccatt
    22801 ccagcacccg gcttagcgtt acgttctcct gaagtagcgg gtgattgggg cggacaacca
    22861 gcttcagcga ttcaagaaac agcagttcat aattaagccc gttcatcagt tcaggatctg
    22921 acatccgacc aatgccgata tcgatttccc cggtttttaa acccgccaga atcatagggt
    22981 tactcattgt cgcaacttgc aaggtcgtct ctttttgttg ctgatgaaac tgacctataa
    23041 ccgaaggtaa tatccctagt gccgcagtag gtagcgcacc aaccctgacg acatcattat
    23101 taagaccttc tttacgatga agcgactgtc cggcagtgtt gatggcgtca agaactctga
    23161 ctgcatgcgt taaaaattgt tcgccgggta aggtaagttg cgccccctga cgaccacgct
    23221 caaacaagcg agcgcccgtc agctgctcca gttcattcaa tgtcttagag agcgcaggtt
    23281 gactcaaatt aagggtttca gccgcgcgcc ccaaagttcc ttgttgtgcg acagctacga
    23341 atgtatgaag gtggcgcaaa cggatgcgct gactaaacag actatttttt tccataagcg
    23401 atgttaaaaa cgaagcggtg tcgctgacaa gtgaagttgt tttattatga taacttgatt
    23461 gcaaaatatt attaacaatt aaagcaacta tgttacagca aaatggataa tattgatgtt
    23521 ttcgcggcga gatcacagtt tgtaaattct tccagccgga gtgaatgcgg ttacctacac
    23581 tccagattac tgaccactgg aggcagacac tatggcgaac agcatcacgg cggatgagat
    23641 tcgggaacag ttttcgcagg caatgtcagc catgtaccag caagaagttc cgcagtacgg
    23701 cacgctgctg gaactggtag ctgatgtaaa tctggcggtg ctggaaaaca atcctcaact
    23761 gcacgaaaaa atggtaaatg cagacgagct ggcgcgactg aatgttgaac gtcatggggc
    23821 gattcgcgtt gggactgcac aagagcttgc tactcttcgg cggatgtttg ccattatggg
    23881 gatgtacccg gtgagctatt acgatctctc gcaggcaggg gtgccggtac attcgacagc
    23941 atttcggccc attgatgatg cttctctggc gcgtaatccc ttccgcgttt ttacctcgtt
    24001 actccgtctt gagcttatcg agaacgaaat tttgcgccag aaagcggcgg agattctgcg
    24061 tcagcgcgat atcttcaccc cacgttgtcg acaactgtta gaggaatatg agcagcaggg
    24121 cggttttaac gaaacacagg cacaggagtt tgtgcaggaa gccctggaaa cgtttcgctg
    24181 gcaccagtca gcaacggtag atgaagaaac ctatcgcgcc ttgcacaacg aacatcggtt
    24241 gattgctgat gtggtctgtt ttcctggatg ccatatcaac catctgacgc cacgtacgct
    24301 ggatattgac cgggtgcagt cgatgatgcc tgaatgcgga attgaaccca aagttctgat
    24361 cgaagggccg ccgcgccgcg aggtgccgat tttactacgc cagaccagct ttaaagcact
    24421 ggaagagacg gtgttgtttg cggggcagaa acagggcaca cataccgcgc gctttggtga
    24481 aattgagcag cgtggtgtgg cattaacgcc gaaagggcga caactgtatg atgatcttct
    24541 gcggaacgct ggaaccgggc aggataatct cactcaccaa atgcatttac aggaaacctt
    24601 ccgcactttt cctgacagtg agtttttaat gcgtcagcaa gggctgggat ggttccggta
    24661 ccgtctgacg ccttcgggtg aggcgcatcg tcaggcgatt catcccggag acgatccaca
    24721 gcccttaata gaacgtggtt gggtagtggc gcaacccatt acctatgaag atttcttgcc
    24781 cgttagcgcg gcggggatct tccagtcaaa tctgggtaat gaaacgcggg catgcaatca
    24841 cggtgatgcc agtcgcgaag catttgagca ggcgttgggg tgtccggttt tggatgagtt
    24901 ccagctttat caggaagcgg aagaacgcag taaacgtcgc tgtggtttgc tttaaattct
    24961 gaccatccgc ctttgcaaaa atttgcctga ttttacaaac gaatcaggct catcccatcg
    25021 acataaaaaa aatgccaatt tatgcatatt ctctgatttc aacaattgga ttattaataa
    25081 atattgtcta gagtgagcgg tcataaataa gcactttctt gccgctgaaa atgaccagcg
    25141 cgggaccatt cacaacacca gaaggactca ctttcaggta tggatcgtag acgatttatt
    25201 aaaggttcaa tggctatggc cgccgtgtgc ggtaccagcg gcattgcttc tcttttttct
    25261 caggcggcat tcgcggccga ttctgatatt gccgacgggc aaacccagcg ttttgacttc
    25321 tccattctac agtcaatggc gcacgactta gcgcaaacag catggcgtgg tgcaccacgt
    25381 ccgttacctg agactctggc gacaatgacg ccgcaggctt ataacagtat tcaatacgac
    25441 gccgaaaaat cgctctggca taacgttgaa aaccgtcaac tggacgctca gtttttccat
    25501 atgggaatgg gattccgtcg ccgcgttcgt atgttttctg tagatccagc aacacatctg
    25561 gcgcgtgaaa ttcactttcg cccggagttg ttcaaataca acgatgcggg tgtggatacc
    25621 aaacaattag aagggcaaag cgatctcggt tttgccggtt ttcgcgtgtt taaagccccc
    25681 gaactggcgc gccgtgatgt agtatcattc ctcggcgcga gttatttccg cgccgttgat
    25741 gacacatatc aatacggttt gtcggcccgc ggcctggcga tcgacactta caccgacagc
    25801 aaagaagagt tccccgactt taccgccttc tggtttgata cggtaaaacc aggggcaacc
    25861 acctttaccg tttatgcgtt gctcgatagc gccagcatta ctggtgccta taagttcact
    25921 atccattgtg agaaaaatca ggtgattatg gatgtggaaa atcacctgta tgcgcgcaaa
    25981 gacattaaac agctgggcat tgcaccgatg accagtatgt tcagctgcgg tactaatgaa
    26041 cgtcggatgt gcgacaccat tcatccgcaa atccatgact ctgatcgtct gtccatgtgg
    26101 cggggcaacg gcgagtggat ttgccgtccg ctgaacaatc cgcaaaaatt gcagttcaat
    26161 gcttacaccg acaacaaccc gaaagggttt ggtttattgc aactggatcg tgatttctcc
    26221 cattatcagg acattatggg ctggtataac aaacgcccaa gtctgtgggt ggaaccgcgt
    26281 aacaagtggg gtaagggcac catcggcctg atggaaatcc caacaacggg cgaaacgctg
    26341 gataacattg tctgcttctg gcagccagaa aaagctgtaa aagcgggtga tgagtttgca
    26401 ttccagtatc gtctgtactg gagtgcgcaa ccgcctgttc attgcccatt agcgcgcgtt
    26461 atggcgacgc gtaccggcat gggcggtttc ccggaaggtt gggcgccagg tgaacactat
    26521 cccgaaaaat gggcgcgacg ttttgccgtc gatttcgttg gtggtgatct gaaagctgcc
    26581 gcgccaaaag gcattgagcc ggtgattacg ctttccagtg gggaagcgaa gcaaatcgaa
    26641 attctctata ttgaacccat tgatggttat cgtattcagt ttgactggta tccgacttcg
    26701 gactccactg atccggtcga tatgcggatg tatctgcgtt gtcagggcga cgctatcagt
    26761 gaaacatggc tgtatcagta tttcccgcca gcgccggata aacgtcagta tgttgatgac
    26821 cgcgtgatga gttaatcgtt ttttcttcgg catcttcttc gggaggtgcc gtctggttaa
    26881 acacgatccc gctcgcattt ttccctaagt taaatgagta atctgatggt gtgtatttca
    26941 gatacacctt gtcagccact aacagggagt gcgtatgttt ccagaatacc gagatttaat
    27001 atcccgtctg aaaaacgaaa atcctcgctt tatgtccttg ttcgataaac acaataaact
    27061 tgatcatgaa attgccagaa aggaaggttc cgacggtcga gggtacaatg cggaagtggt
    27121 ccgcatgaaa aaacaaaagc tacagttaaa agatgagatg ctcaaaatcc tgcagcaggt
    27181 gagcgtcaaa gaggtgtaaa ctttcctaag ccgccagcca ggcggctttt ttaacagctg
    27241 catggattga ctggagataa gatgactgaa acgataaaag taagcgaatc acttgaatta
    27301 catgctgttg cggaaagtca tgtcacacct ctttatcagt taatctgtaa aaataaaacc
    27361 tggttacagc agtcgctaaa ctggccgcag tttgttcaaa gtgaagagga cacgcgaaaa
    27421 acggtgcagg gtaatgtgat gttgcatcaa cgcggctatg ccaaaatgtt catgattttc
    27481 aaagaagatg aacttatcgg cgttatctct tttaatcgta ttgaaccact gaataaaacc
    27541 gctgaaatcg gctactggct cgacgaatct catcaggggc aggggatcat ttctcaggcg
    27601 ctgcaggcat tgattcatca ttacgctcag tctggtgaac ttagacgctt cgtgatcaaa
    27661 tgtcgggtgg acaatccgca aagcaaccag gtagcgttgc gcaatggttt tatccttgaa
    27721 ggttgcctta aacaggctga gttcctgaat gatgcctatg atgatgtgaa cttatacgcg
    27781 cgtattatcg attcacaata accctgccag cagcgttcgc gtaatgcgct cctcgccgtt
    27841 aatgactttt ggaccacgga tatgaatcgt gtcgccatca aaggcttcaa cgacggcgtg
    27901 gtcagttaac tccacatggt tttcaattat caccgcacct gtaatacggc tctcaccttg
    27961 tatgacaacg tgctcgtcga gcagaatcgg tccgccacat accacggcat ttccgccaat
    28021 caagacatga tgttttaaca cacaattacc ttccacaatg gcatattccg ccacctgcga
    28081 actgtaatga atcgtcggaa tggcatcttc ttctatgcca gctttcacct gcgcgtgacc
    28141 gtagacttta gcgcaatcgc atagccagac gttgttctct tcattacctt caacgctggc
    28201 aaaatcaaaa acttcggcgc gatgttcaat aaaagcatac cggacgacgg catcgccata
    28261 aatttgtgcc tggtggacaa tacgcgaggc gctaaccttt gcgcgatcat aaatttgtag
    28321 caggagttga tggtcgggcg ttaagccttg tgcggcgacg atcatagaat gttgatcaat
    28381 taaggcgtga ccaaatattc tacattgacc gcaaactaat gagtcacgaa tggtgacgct
    28441 gtcacttata tatgcaccct ggctgatttc actattatcg atccagacat tatccgttgc
    28501 gtaaacctct ccccacagca cgctggtgcc ggtgatacgc gtattcccgg aaatcaccgt
    28561 tccgccgaac gcaatagcat tctggtcgta aatccagcaa tttccttcct gtgctaacac
    28621 cgtttcgtga tcgatccagc cgccagcgct tcctgctatc acatcgttaa aatcgctcat
    28681 ggcgataatt tgccgtaata acacactttt tttagtgcca tcttcctgat aactaaaggc
    28741 tcgctgttct tcacttagac gatatttacg catagcgttt tcccacaggt gacttactat
    28801 aaccgtagca aattctgcgg ctctggctat gctcgagaaa attccataaa atgcatttca
    28861 aa
//
LOCUS       DADSYM010000052        28851 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2415
            SAMN08039579-rid11723173.denovo.052, whole genome shotgun sequence.
ACCESSION   DADSYM010000052 DADSYM010000000
VERSION     DADSYM010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039579
            Sequence Read Archive: SRR6362533
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 28851)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28851)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980162.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 03:39:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,484
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 4,347
            CDSs (with protein)               :: 4,347
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 43 of 85
            Pseudo Genes (incomplete)         :: 37 of 85
            Pseudo Genes (internal stop)      :: 19 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28851
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039579-rid11723173.denovo.052"
                     /strain="LGE2415"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8302 N 95.9628 W"
                     /collection_date="2015-08-24"
     gene            93..950
                     /gene="focA"
                     /locus_tag="J1Y83_001888"
     CDS             93..950
                     /gene="focA"
                     /locus_tag="J1Y83_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate transporter FocA"
                     /protein_id="HBA7630038.1"
                     /translation="MKADNPFDLLLPAAMAKVAEEAGVYKATKHPLKTFYLAITAGVF
                     ISIAFVFYITATTGTGTMPFGMAKLVGGICFSLGLILCVVCGADLFTSTVLIVVAKAS
                     GRITWGQLAKNWLNVYFGNLVGALLFVLLMWLSGEYMTANGQWGLNVLQTADHKVHHT
                     FIEAVCLGILANLMVCLAVWMSYSGRSLMDKAFIMVLPVAMFVASGFEHSIANMFMIP
                     MGIVIRDFASPEFWTAVGSAPENFSHLTVMNFITDNLIPVTIGNIIGGGLLVGLTYWV
                     IYLRENDHH"
     gene            1005..3287
                     /gene="pflB"
                     /locus_tag="J1Y83_001889"
     CDS             1005..3287
                     /gene="pflB"
                     /locus_tag="J1Y83_001889"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HBA7630039.1"
                     /translation="MSELNEKLATAWEGFTKGDWQNEVNVRDFIQKNYTPYEGDESFL
                     AGATEATTTLWDKVMEGVKLENRTHAPVDFDTAVASTITSHDAGYINKQLEKIVGLQT
                     EAPLKRALIPFGGIKMIEGSCKAYNRELDPMIKKIFTEYRKTHNQGVFDVYTPDILRC
                     RKSGVLTGLPDAYGRGRIIGDYRRVALYGIDYLMKDKLAQFTSLQADLENGVNLEQTI
                     RLREEIAEQHRALGQMKEMAAKYGYDISGPATNAQEAIQWTYFGYLAAVKSQNGAAMS
                     FGRTSTFLDVYIERDLKAGKITEQEAQEMVDHLVMKLRMVRFLRTPEYDELFSGDPIW
                     ATESIGGMGLDGRTLVTKNSFRFLNTLYTMGPSPEPNMTILWSEKLPLNFKKFAAKVS
                     IDTSSLQYENDDLMRPDFNNDDYAIACCVSPMIVGKQMQFFGARANLAKTMLYAINGG
                     VDEKLKMQVGPKSEPIKGDVLNYDEVMERMDHFMDWLAKQYITALNIIHYMHDKYSYE
                     ASLMALHDRDVIRTMACGIAGLSVAADSLSAIKYAKVKPIRDEDGLAIDFEIEGEYPQ
                     FGNNDPRVDDLAVDLVERFMKKIQKLHTYRDAIPTQSVLTITSNVVYGKKTGNTPDGR
                     RAGAPFGPGANPMHGRDQKGAVASLTSVAKLPFAYAKDGISYTFSIVPNALGKDDEVR
                     KTNLAGLMDGYFHHEASIEGGQHLNVNVMNREMLLDAMENPEKYPQLTIRVSGYAVRF
                     NSLTKEQQQDVITRTFTQSM"
     gene            complement(3610..3828)
                     /gene="ogrK"
                     /locus_tag="J1Y83_001890"
     CDS             complement(3610..3828)
                     /gene="ogrK"
                     /locus_tag="J1Y83_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage transcriptional regulator OgrK"
                     /protein_id="HBA7630040.1"
                     /translation="MFHCPLCQHAAHARTSRYITDTTKERYHQCQNVNCSATFITYES
                     VQRYIVKPGEVHAVRPHPLPSGQQIMWM"
     gene            complement(3910..5073)
                     /locus_tag="J1Y83_001891"
     CDS             complement(3910..5073)
                     /locus_tag="J1Y83_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016157990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage late control D family protein"
                     /protein_id="HBA7630041.1"
                     /translation="MLDALTFDAGSTLTPDYMLMLDSRDITGNISDRLMSMTLTDNRG
                     FEADQLDIELNDADGQVGLPVRGAVLTVYIGWKGFALVCKGKFTVDEVEHRGAPDVVT
                     IRARSADFRGTLNSRREGSWHDTTLGAIVEVIASRNRLEASVAPSLAGIKIPHIDQSQ
                     ESDAKFLTRLAERNGGEVSVKMGKLLFLKAGQGVTASGKKIPQVTITRSDGDRHHFAI
                     ADRGAYTGVTAKWLHTKDPKPQKQKVKLKRKKKEKHLRALEHPKAKPVRQKKAPKVPE
                     AREGEYMAGEADNVFALTTVYATKAQAMRAAQAKWDKLQRGVAEFSISLATGRADIYT
                     ETPVRVSGFKRVIDEQDWTITKVTHFLNNSGFTTSLELEVRLSDVEYETEDDE"
     gene            complement(5073..5552)
                     /locus_tag="J1Y83_001892"
     CDS             complement(5073..5552)
                     /locus_tag="J1Y83_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000978924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBA7630042.1"
                     /translation="MMLALGMFVFMRQTLPHQTMQRESDYRWPSNSRIGKRDAFQFLG
                     VGEENMTLAGVLYPELTGGKLTMTTLRLMAEEGRAWPLLDGTGMIYGMYVISKVSETG
                     SIFFADGTPRKIDFTLSLTRVDESLAALYGDIGKQAESLIGKAGSMATKLTGMTGAG"
     gene            complement(5567..8014)
                     /locus_tag="J1Y83_001893"
     CDS             complement(5567..8014)
                     /locus_tag="J1Y83_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016236410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HBA7630043.1"
                     /translation="MSNNVKLQVLLRAVDQASRPFKSIRTASKSLSGDIRETQKSLRE
                     LNGHASRIEGFRKTSAQLAVTGHALEKARQEAEALATQFKNTERPTRAQAKVLESAKR
                     AAEDLQAKYNRLTDSVKRQQRELAAVGINTRNLAHDEQGLKNRISETTAQLNRQRDAL
                     ARVSAQQAKLNAVKQRYQAGKELAGNMASVGAAGVGIAAAGTMAGVKLLMPGYEFAQK
                     NSELQAVLGVAKDSTEMAALRKQARQLGDNTAASADDAAGAQIIIAKAGGDVDAIQAA
                     TPVTLNMALANRRTMEENAALLMGMKSAFQLSNDKVAHIGDVLSMTMNKTAADFDGMS
                     DALTYAAPVAKNAGVSIEETAAMVGALHDAKITGSMAGTGSRAVLSRLQAPTGKAWDA
                     LKELGVKTSDSKGNTRPIFTILKEMQASFEKNRLGTAQQAEYMKTIFGEEASSAAAVL
                     MTAASTGKLDKLTAAFKASDGKTAELVNIMQDNLGGDFKEFQSAYEAVGTDLFDQQEG
                     ALRKLTQTATKYVLKLDGWIQKNKSLASTIGIIAGGALALTGIIGAIGLVAWPVITGI
                     NAIIAAAGAMGAIFTTVGSAVMTAIGAISWPVVAVVAAIVAGALLIRKYWEPVSAFFG
                     GVVEGLKAAFAPVGELFTPLKPLFDWLGEKLQAAWQWFKNLIAPVKATQDTLNRCRDT
                     GVMFGQALADALMLPLNAFNKLRSGIDWVLEKLGVINKESDTLDQTAARTQAATYGTG
                     GYIPATSSYAGYQAYQPVTAPAGRSYVDQSKNEYHISLTGGNAPGIQLDRQLQDALEK
                     YERDKRARARASMMHDG"
     gene            complement(8007..8126)
                     /locus_tag="J1Y83_001894"
     CDS             complement(8007..8126)
                     /locus_tag="J1Y83_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005165322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GpE family phage tail protein"
                     /protein_id="HBA7630044.1"
                     /translation="MADVAVIFHWPPSELYPMSLTELITWREKALQRSGNTNE"
     gene            complement(8159..8434)
                     /locus_tag="J1Y83_001895"
     CDS             complement(8159..8434)
                     /locus_tag="J1Y83_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001719230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein"
                     /protein_id="HBA7630045.1"
                     /translation="MNKENVITLDNPVKRGEQVIEQVTLMKPNAGTLRGVSLAAVANS
                     EVDALIKVLPRMTAPMLTEQEVAALELPDLVALAGKVVGFLSPNSVQ"
     gene            complement(8491..9009)
                     /locus_tag="J1Y83_001896"
     CDS             complement(8491..9009)
                     /locus_tag="J1Y83_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001554312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major tail tube protein"
                     /protein_id="HBA7630046.1"
                     /translation="MAMPRKLKLMNVFLNGYSYQGVAKSITLPKLTRKLENYRGAGMN
                     GSAPVDLGLDDDALSMEWSLGGFPDSVIWELYAATGVDAVPIRFAGSYQRDDTGETVA
                     VEVVMRGRQKEIDTGEGKQGEDTESKISVVCTYFRLTMDGKELVEIDTINMIEKVNGV
                     DRLEQHRRNIGL"
     gene            complement(9022..10212)
                     /locus_tag="J1Y83_001897"
     CDS             complement(9022..10212)
                     /locus_tag="J1Y83_001897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001483446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath protein"
                     /protein_id="HBA7630047.1"
                     /translation="MSDYHHGVQVLEINDGTRVISTVSTAIVGMVCTASDADAETFPL
                     NKPVLITNVQSAIAKAGKKGTLAASLQAIADQSKPVTVVVRVEDGTGDDEETKLAQTV
                     SNIIGTTDENGQYTGLKALMGAESVTGVKPRILGVPGLDTKEVAVALASVSQELNAFG
                     YISAWGCKTISEVKAYRQNFSQRELMVIWPDFLAWDTVTSTTATAYATARALGLRAKI
                     DQEQGWHKTLSNVGVNGVTGISASVFWDLQKPGTDADLLNESGVTTLIRRDGFRFWGN
                     RTCSDDPLFLFESYTRTAQVLADTMAEAHMWAIDKPITATLIRDIIDGINAKFRELKN
                     NGYIVDGTCWFSEESNDAETLKAGKLYIDYDYTPVPPLENLTLRQRITSRYLASLVTS
                     VNSN"
     gene            complement(10541..11509)
                     /locus_tag="J1Y83_001898"
     CDS             complement(10541..11509)
                     /locus_tag="J1Y83_001898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004200835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage exclusion protein"
                     /protein_id="HBA7630048.1"
                     /translation="MRGRYNALLYAIGGLVETTDNVRKLFDGVVPERASEIESIANDY
                     DAQFRLIGDREGFNLDAGGFSAIQYTSRSMRQIWLFSYAGMQALHCYCSLIELFKSCG
                     ATLDINEINKIPDQAAEDEAFKSILDAVKDLSTAFHEDDFEWPVAIPEPEKGRPSDSE
                     SAAVYDLMCMATAYVFLHELKHVIFSVEGNAPEDPKDEEYLCDQFAKDMMISKIDEYA
                     ASSGYPPEKVRMKRMMGIALAFAFILFATGRNRLAGSETHPPIYGRWSATVQDVNLPE
                     DDWFWLYFSSFALALLKYHSITIRPKIVKDYKSLCFDLIADLENGI"
     gene            complement(11690..12217)
                     /locus_tag="J1Y83_001899"
     CDS             complement(11690..12217)
                     /locus_tag="J1Y83_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001164091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HBA7630049.1"
                     /translation="MQHLINITAGNPKTVEQYQLTRDFDVVWFFSEDGKNWYEEQKYF
                     ADDTIKIAYDKDNIIRYVEKDVTAIRPDGLSVVEVADITANRRADISGNWMFKDGKVI
                     KRIYTAEELQQQAENRKARLLADAESVILPLERAVRLNMATDEERSRLKTWERYSVLV
                     SRVDPANPEWPEMPQ"
     gene            complement(12221..14539)
                     /locus_tag="J1Y83_001900"
     CDS             complement(12221..14539)
                     /locus_tag="J1Y83_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001623412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBA7630050.1"
                     /translation="MSTKFRTVITTAGAAKLAAATAPGGRKVNITTMAVGDGGGKLPV
                     PDAGQTGLIHEVWRHALNKISQDKRNSNYIIAELVIPPEVGGFWMRELGLYDDAGTLI
                     AVANMAESYKPALAEGSGRSQTCRMVIIVSSVASVELTIDTTTVMATQDYVDDKIAEH
                     EQSRRHPDASLTAKGFTQLSSATNSTSETLAATPKAVKTAYDLANGKYTAQDATTARK
                     GLVQLSSATNSTSETLAATPKAVKAAYDLANGKYTAQDATTARKGLVQLSSVTNSDSE
                     TLAATPKAVKTAYDLANGKYTAQDATTARKGLVQLSSATNSDSETLAATPKAVKSAYD
                     NAEKRLQKDQNGADIPGKDTFTKNIGACRAYSGALSTEAGNWTTAQFIEWLDSRGAFN
                     HPYWMCKGSWSYANNKIITDTGCGDIHLAGCVVEVMGTKSAITIRVTTPTTSSGGGTT
                     SAQFTYINHGDGYSPGWRRDWNRQGDAMTGTINQDGGSQNTYMSTALCSGTRGGKKYL
                     RKFRGGEGDTIWHETVQGGVVRWATGNTDAQEELSLSSAYGLRSRGEITSSSANGLRI
                     AYGNYGFFIRNDGSSTYFMLTKSGDRLGTYNNLRPLIINDATGAVSMGHGLSVTGDIV
                     SSTKVRAGSGKKFTVSSSNTSTKEAAFNLWGNSSRPVVAELGDDAGWHFYSQRNTDNS
                     ITFAVNGQVSPSNYGNFDSRYVRDIRLGGAATYKPANNGMTWTHQAPSGCVYSGIIVQ
                     DTGSNSADNIGGVYYRPVQKYINGTWYNVAQV"
     gene            complement(14550..15080)
                     /locus_tag="J1Y83_001901"
     CDS             complement(14550..15080)
                     /locus_tag="J1Y83_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001285325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein I"
                     /protein_id="HBA7630051.1"
                     /translation="MSDSRLLPTGSSPLEIAAAKACAEIEKTPVSIRELWNPDTCPAN
                     LLPWLAWAFSVDRWDEKWPEATKRAVIRDAYFIHCHKGTIGAIRRVVEPLGYLINVTE
                     WWENSDPPGTFRLDIGVLESGITEAMYQEMERLIADAKPASRHLIGLNITRDIPGYLF
                     AGGVAYDGDVITVYPG"
     gene            complement(15073..15981)
                     /locus_tag="J1Y83_001902"
     CDS             complement(15073..15981)
                     /locus_tag="J1Y83_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000246670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate assembly protein"
                     /protein_id="HBA7630052.1"
                     /translation="MPIIDLNQLPAPDVVEALDFETILAERKATLISLYPEDQQEAVA
                     RTLTLESEPLVKLLEENAYRELIWRQRVNEAARAVMLACAAGNDLDVIGANYNTTRLT
                     ITPADDSTIPPTPAVMESDTDYRLRIQQAFEGLSVAGSVGAYQYHGRSADGRVADISV
                     TSPSPACVTISVLSRENNGVASEDLLAVVRNALNGEDVRPVADRVTVQSAAIVEYQIN
                     ATLYLYPGPESEPIRAAAVKKLEAYITAQHRLGRDIRLSAIYAALHVEGVQRVELAAP
                     LADIVLNSTQASFCTEYRVVTGGSDE"
     gene            complement(15986..16333)
                     /locus_tag="J1Y83_001903"
     CDS             complement(15986..16333)
                     /locus_tag="J1Y83_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005228331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate assembly protein"
                     /protein_id="HBA7630053.1"
                     /translation="MTARYLGMNRSDGLTVTDLEHISQSIGDILRTPVGSRVMRRDYG
                     SLLASMIDQPQTPALELQIKVACYMAVLKWEPRVTLSSVTTERSFDGRMTVTLTGQHN
                     DTGQPLSLTIPVS"
     gene            complement(16330..16971)
                     /locus_tag="J1Y83_001904"
     CDS             complement(16330..16971)
                     /locus_tag="J1Y83_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001093709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate assembly protein V"
                     /protein_id="HBA7630054.1"
                     /translation="MNTLANIQELARALRNMIRTGLVVETNLKAGRCRVQTGGMCTDW
                     LQWLTHRAGRSRTWWAPSVGEQVLILSVGGELDTAFVLPGIYSGDNPAPSASADALYI
                     RFPDGAVIEYEPETSALTASGIKTASVTASDSVTATVPVVMVKASTRITLDTPEVVCT
                     NKLTTGTLEVQKGGKMHGNIEHTDGKFTSNGVQVDDHGHGGVKSGDNWTQGTK"
     gene            17055..17840
                     /locus_tag="J1Y83_001905"
     CDS             17055..17840
                     /locus_tag="J1Y83_001905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001704934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA7630055.1"
                     /translation="MVSYNVTNVWELIVLYLLVFVVLAFFSFGKSNLMRLIAHYFKFG
                     YSDKKLKRLDREWRDIQLFKIINGINVSGIKDVKMIQQGLIDGKLKTSDFFLTRFWGD
                     ITEPPCITKTMSAVLAGILYITFACCIHNEQSAIVEDAIAIPYKNMMYHVYNDKVLLF
                     SDNKTDKFYKAFSIADCKRLQNTFVTDTLPAIACNKLLQLNEEDSEWLSQEIKDNNSH
                     RKTLLIISLVYFTSGLVILLSYTKFLYANKKVVEYKSSNKNHS"
     gene            complement(17912..18364)
                     /locus_tag="J1Y83_001906"
     CDS             complement(17912..18364)
                     /locus_tag="J1Y83_001906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001001795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage virion morphogenesis protein"
                     /protein_id="HBA7630056.1"
                     /translation="MNEFKRFEDRLTGLIEPLSPSGRRRLSAELAKRLRQSQQRRVMA
                     QKAPDGTPYAPRQQQSARKKTGRVKRKMFSKLITSRFLHIRASPEQASMEFYGGKSPK
                     IASVHQFGLSEETRKDGKKIDYPARPLLGFTGEDVQMIEEIILAHLER"
     gene            complement(18357..18824)
                     /locus_tag="J1Y83_001907"
     CDS             complement(18357..18824)
                     /locus_tag="J1Y83_001907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001406878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBA7630057.1"
                     /translation="MLKPDSLRRALTDAVTVLKTNPDMLRIFVDNGSIASTLAASLSF
                     EKRYTLNVIVTDFTGDFDLLIVPVLAWLRENQPDIMTTDEGQKKGFTFYADINNDSSF
                     DISISLMLTERTLVSEVDGALHVKNIPEPPPPEPVTRPMELYINGELVSKWDE"
     gene            complement(18787..18960)
                     /locus_tag="J1Y83_001908"
     CDS             complement(18787..18960)
                     /locus_tag="J1Y83_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001300730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage lysis protein"
                     /protein_id="HBA7630058.1"
                     /translation="MGLCPMPGSDPETNGDLSADIRQLENALARCASQVKMIKHCQDE
                     NDAQTRQPAQGAD"
     gene            complement(18932..19357)
                     /locus_tag="J1Y83_001909"
     CDS             complement(18932..19357)
                     /locus_tag="J1Y83_001909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001585210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysB family phage lysis regulatory protein"
                     /protein_id="HBA7630059.1"
                     /translation="MSKLMIVLVVLLSLAVAGLFLAKHENASLRASLDRVNNVASEQQ
                     TTITMLKNQLHVALTRADKNELAQVALRQELENAAKREAQREKTITRLLNENEDFRRW
                     YGAGLPDAVRRLHQRPACTDASDCPQRLPESGPLPDAGQ"
     gene            complement(19345..19770)
                     /locus_tag="J1Y83_001910"
     CDS             complement(19345..19770)
                     /locus_tag="J1Y83_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000736575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein lysA"
                     /protein_id="HBA7630060.1"
                     /translation="MKKLSLSLMLHVSLALMLALSLIYPQSVAVSFVAAWAILATVIC
                     VVAGGVGVYATEYALERYGWELPPESLAVKIVMSLFLQPVPWCRRAAALVVMVATFIS
                     LVAAGWIFTALIYLVASVFFRLIRTACRQRLEGREPCQS"
     gene            complement(19785..20282)
                     /locus_tag="J1Y83_001911"
     CDS             complement(19785..20282)
                     /locus_tag="J1Y83_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016235441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 104 protein"
                     /protein_id="HBA7630061.1"
                     /translation="MPVINTHQNIAAFLDMLAVSEGTANHPLTKNRGYDVIVTGLDGK
                     PEIFTDYSDHPFAHGRPAKVFNRRGEKSTASGRYQQLYLFWPHYRKQLALPDFSPLSQ
                     DRLAIQLIRERGALDDIRAGRIERAISRCRNIWASLPGAGYGQREHSLEKLVTVWRTA
                     GGVPA"
     gene            complement(20282..20563)
                     /locus_tag="J1Y83_001912"
     CDS             complement(20282..20563)
                     /locus_tag="J1Y83_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000123124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holin"
                     /protein_id="HBA7630062.1"
                     /translation="MTAEEKSVLSLFMIGVLIVVGKVLAGGEPITPRLFIGRMLLGGF
                     VSMVAGVVLVQFPDLSLPAVCGIGSMLGIAGYQVIEIAIQRRFKGRGKQ"
     gene            complement(20567..20770)
                     /locus_tag="J1Y83_001913"
     CDS             complement(20567..20770)
                     /locus_tag="J1Y83_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005228323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBA7630063.1"
                     /translation="MKTFALQGDTLDAICVRYYGRTEGVVEAVLAANPGLVELGAVLP
                     HGTAVELPDVQTAPVAETVNLWE"
     gene            complement(20770..21279)
                     /locus_tag="J1Y83_001914"
     CDS             complement(20770..21279)
                     /locus_tag="J1Y83_001914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005228322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head completion/stabilization protein"
                     /protein_id="HBA7630064.1"
                     /translation="MMTLIIPRKEAPVSGEGTVVIPQPAGDEPVIKNTFFFPDIDPKR
                     VRERMRLEQTVAPARLREAIKSGMAETNAELYEYREQKIAAGFTRLADVPADDIDGES
                     IKVFYYERAVCAMATASLYERYRGVDASAKGDKKADSIDSTIDELWRDMRWAVARIQD
                     KPRCIVSQI"
     gene            complement(21379..22122)
                     /locus_tag="J1Y83_001915"
     CDS             complement(21379..22122)
                     /locus_tag="J1Y83_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001540320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase endonuclease subunit"
                     /protein_id="HBA7630065.1"
                     /translation="MTSPAQRHMMRVSAAMTAQREAAPLRHATVYEQMLVKLAADQRT
                     LKAIYSKELKAAKKRELLPFWLPWVNGVLEQGKGAQDDILMTVMLWRLDTGDIAGALE
                     IARYALKYGLTMPGKHRRTPPYMFTEEVALAAMRAHAAGESVDTRLLTETLELTATAD
                     MPDEVRAKLHKITGLFLRDGGDAAGALAHLQRATQLDCQAGVKKEIERLERELKPKPE
                     PQPKAATRTPRKTRSVTPAKRGRPKKKAS"
     gene            complement(22126..23199)
                     /locus_tag="J1Y83_001916"
     CDS             complement(22126..23199)
                     /locus_tag="J1Y83_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001248534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein, P2 family"
                     /protein_id="HBA7630066.1"
                     /translation="MRQETRFKFNAYLSRVAELNGIDAGDVSKKFTVEPSVTQTLMNT
                     MQESSDFLTRINIVPVSEMKGEKIGIGVTGSIASTTDTAGGTERQPKDFSKLASNKYE
                     CDQINFDFYIRYKTLDLWARYQDFQLRIRNAIIKRQSLDFIMAGFNGVKRAETSDRSS
                     NPMLQDVAVGWLQKYRNEAPARVMSKVTDEEGHTTSEVIRVGKGGDYASLDALVMDAT
                     NNLIEPWYQEDPDLVVIVGRQLLADKYFPIVNREQDNSEMLAADVIISQKRIGNLPAV
                     RVPYFPADAMLITKLENLSIYYMDDSHRRVIEENPKLDRVENYESMNIDYVVEDYAAG
                     CLVEKIKVGDFSTPAKATAEPGA"
     gene            complement(23258..24112)
                     /locus_tag="J1Y83_001917"
     CDS             complement(23258..24112)
                     /locus_tag="J1Y83_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001074418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GPO family capsid scaffolding protein"
                     /protein_id="HBA7630067.1"
                     /translation="MAKKVSKFFRIGVEGDTCDGRVISAQDIQEMAETFDPRVYGCRI
                     NLEHLRGILPDGIFKRYGDVAELKAEKIDDDSALKGKWALFAKITPTDDLIAMNKAAQ
                     KVYTSMEIQPNFANTGKCYLVGLAVTDDPASLGTEYLEFCRTAKHNPLNRFKLSPENL
                     ISVATPVELEFEDLPETVFTALTEKVKSIFGRKQASDDARLNDVHEAVTAVAEHVQEK
                     LSATEQRLAEMETAFSALKQDVTDRADETSQAFTRLKNSLDHTESLTQQRRSKATGGS
                     GDALLTNC"
     gene            24286..26058
                     /locus_tag="J1Y83_001918"
     CDS             24286..26058
                     /locus_tag="J1Y83_001918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001357052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase ATPase subunit family protein"
                     /protein_id="HBA7630068.1"
                     /translation="MTITTDTTLLHDPRRQAALLYWQGFSVPQIAAMLQMKRPTVQSW
                     KQRDGWDSVAPISRVEMSLEARLTQLIIKPQKTGSDFKEIDLLGRQIERLARVNRYSQ
                     TGNEADLNPNVANRNKGGRRKPKKNFFSDEAIEKLEQIFFEQSFDYQLHWYRAGLEHR
                     IRDILKSRQIGATFYFSREALLRALKTGHNQIFLSASKTQAYVFREYIIAFARLVDVD
                     LTGDPIVLGNNGAKLIFLGTNSNTAQSHNGDLYVDEIFWIPNFQVLRKVASGMASQSH
                     LRSTYFSTPSTLAHDAYPFWSGELFNRGRASAAERVEIDVSHNALAGGLLCADGQWRQ
                     IVTIEDALKGGCTLFDIEQLKRENSADDFKNLFMCEFVDDKASVFPFEELQRCMVDTL
                     EEWEDYAPFAANPFGSRPVWIGYDPSHRGDSAGCVVLAPPVVAGGKFRILERHQWKGM
                     DFATQAESIRKLTEKYNVEYIGIDATGLGVGVFQLVRSFYPAARDIRYTPEMKTAMVL
                     KAKDVIRRGCLEYDVSATDITSSFMAIRKTMTSSGRSATYEASRSEEASHADLAWATM
                     HALLNEPLTAGISTPLTSTILEFY"
     gene            26058..27092
                     /locus_tag="J1Y83_001919"
     CDS             26058..27092
                     /locus_tag="J1Y83_001919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001752368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HBA7630069.1"
                     /translation="MSKKKGKTPQPAAKKMTASAPKMEAFTFGEPVPVLDRRDILDYV
                     ECISNGRWYEPPVSFTGLAKSLRAAVHHSSPIYVKRNILASTFIPHPWLSQQDFSRFV
                     LDFLVFGNAFLEKRYSTTGKVIRLETSPAKYTRRGVEEDVYWWVPSFNEPTAFAPGSV
                     FHLLEPDINQELYGLPEYLSALNSAWLNESATLFRRKYYENGAHAGYIMYVTDAVQDR
                     NDIEMLRENMVKSKGRNNFKNLFLYAPQGKADGIKIIPLSEVATKDDFFNIKKASAAD
                     LLDAHRIPFQLMGGKPENVGSLGDIEKVAKVFVRNELIPLQDRIREINGWLGQEVIRF
                     KNYSLDTDNG"
     gene            complement(27131..27316)
                     /locus_tag="J1Y83_001920"
     CDS             complement(27131..27316)
                     /locus_tag="J1Y83_001920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA7630070.1"
                     /translation="MHLHQNRPMKRAGEAGKALRAGGGADFIFSASQRVVTALSLPVE
                     ALVCLRGVLCGGERVRA"
     gene            complement(27484..27936)
                     /locus_tag="J1Y83_001921"
     CDS             complement(27484..27936)
                     /locus_tag="J1Y83_001921"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA7630071.1"
                     /translation="MDFKNSLAVGLEAAKKARSNKEEIFSVISDLNDSVKELSQGRVF
                     LKVTSEKKLAPNQTALTAVVSSFTNINPYIDYEALSFVTKREQTEVKENIAVWKINES
                     DGYPCVISYNKRDVSCKDKETLIKALNDLMSDASTGEILLRLMNTEEA"
     gene            complement(27941..28843)
                     /locus_tag="J1Y83_001922"
     CDS             complement(27941..28843)
                     /locus_tag="J1Y83_001922"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HBA7630072.1"
                     /translation="MCEAVSIAWWNTGISPAVKRDRSTPEELDVAFALITNILMSHNV
                     DILCLGEISPSDIKVLEEIFSEKGFIIYDGTFTEGSIKHDMCILIKLATFTYIDSKSI
                     VEQSLLGAVRAGQEVQLMHNNSGDHFFFYISHWPMRGNDSNEEMPKRYELGKALRGAI
                     SRCIEEKSGKYFILLGDYNDDPFNKSITHALGATRDRDLVVKKDIFLYNPFWNFMGAS
                     ISFPSEDHHLSTYGTYYYSGGTVTRWHTFDQIIFSSSFISGGDWNLLEDEVKIINDEV
                     FKNLVTSPKTNFDHLPVIAVITRG"
BASE COUNT         6615 a         7979 c         7162 g         7095 t
ORIGIN      
        1 ctataagcca ggcgagatat gatctatatc aatttctcat ctataatgct ttgttagtat
       61 ctcgtcgccg acttaataaa gagagagtta gtgtgaaagc tgacaaccct tttgatcttt
      121 tacttcctgc tgcaatggcc aaagtggccg aagaggcagg tgtctataaa gcaacgaaac
      181 atccgcttaa gactttctat ctggcgatta ccgccggtgt tttcatctca atcgcattcg
      241 tcttctatat cacagcaacc actggcacag gtacaatgcc cttcggcatg gcaaaactgg
      301 ttggcggcat ttgcttctct ctggggttga ttctttgtgt tgtctgcgga gccgatctct
      361 ttacttccac agtgttgatt gttgttgcca aggcgagtgg gcgcatcact tggggtcagt
      421 tggcgaaaaa ctggctaaat gtttattttg gcaacctggt cggcgcactg ctgtttgtac
      481 ttttaatgtg gctttccggc gagtatatga ccgcaaatgg tcaatgggga ctaaacgtcc
      541 tacaaaccgc cgaccacaaa gtgcaccata cttttattga ggccgtctgt cttggtatcc
      601 tggcaaacct gatggtatgt ctggcagtat ggatgagtta ttctggccgc agcctgatgg
      661 acaaagcgtt cattatggtg ctgccggtcg cgatgtttgt tgccagcggt tttgagcaca
      721 gtatcgcaaa catgtttatg atcccgatgg gtattgtaat ccgcgacttc gcatctccgg
      781 aattctggac cgctgtcggt tctgcaccgg aaaatttttc tcacctgacc gtgatgaact
      841 tcatcactga taacctgatt ccggttacga tcggtaatat tatcggcggt ggtttgttgg
      901 ttgggttgac atactgggtc atttacctgc gtgaaaacga tcaccattaa tggttgtcga
      961 agtacgcagt aaataaaaaa tccacttaag aaggtaggtg ttacatgtcc gagcttaatg
     1021 aaaagttagc cacagcctgg gaaggtttta ccaaaggtga ctggcagaat gaagtaaacg
     1081 tccgtgactt cattcagaaa aactacactc cgtacgaggg tgacgagtcc ttcctggctg
     1141 gcgctactga agcgaccacc accctgtggg acaaagtaat ggaaggcgtt aaactggaaa
     1201 accgcactca cgcgccagtt gactttgaca ccgctgttgc ttccaccatc acctctcacg
     1261 acgctggtta catcaacaag cagcttgaga aaatcgttgg tctgcagact gaagctccgc
     1321 tgaaacgtgc tcttatcccg ttcggtggta tcaaaatgat cgaaggttcc tgcaaagcgt
     1381 acaaccgcga actggacccg atgatcaaaa aaatcttcac tgaataccgt aaaactcaca
     1441 accagggcgt gttcgacgtt tacactccgg acatcctgcg ttgccgtaaa tccggtgttc
     1501 tgaccggtct gccagatgct tatggccgtg gtcgtatcat cggtgactac cgtcgcgttg
     1561 cgctgtacgg tatcgactac ctgatgaaag acaaacttgc acagttcacc tctctgcagg
     1621 ctgatctgga aaacggcgta aacctggaac agactatccg tctgcgcgaa gaaatcgctg
     1681 aacagcaccg cgctctgggt cagatgaaag aaatggctgc gaaatacggc tacgacatct
     1741 ctggtccggc taccaacgct caggaagcta tccagtggac ttacttcggc tacctggctg
     1801 ctgttaagtc tcagaacggt gctgcaatgt ccttcggtcg tacctccacc ttcctggatg
     1861 tgtacatcga acgtgacctg aaagctggca agatcaccga acaagaagcg caggaaatgg
     1921 ttgaccacct ggtcatgaaa ctgcgtatgg ttcgcttcct gcgtactccg gaatacgatg
     1981 aactgttctc tggcgacccg atctgggcaa ccgaatctat cggtggtatg ggcctcgacg
     2041 gtcgtaccct ggttaccaaa aacagcttcc gtttcctgaa caccctgtac accatgggtc
     2101 cgtctccgga accgaacatg accattctgt ggtctgaaaa actgccgctg aacttcaaga
     2161 aattcgccgc taaagtgtcc atcgacacct cttctctgca gtatgagaac gatgacctga
     2221 tgcgtccgga cttcaacaac gatgactacg ctattgcttg ctgcgtaagc ccgatgatcg
     2281 ttggtaaaca aatgcagttc ttcggtgcgc gtgcaaacct ggcgaaaacc atgctgtacg
     2341 caatcaacgg tggcgttgac gaaaaactga aaatgcaggt tggtccgaaa tctgagccga
     2401 tcaaaggcga tgtcctgaac tatgatgaag tgatggagcg catggatcac ttcatggact
     2461 ggctggctaa acagtacatc actgcactga acatcatcca ctacatgcac gacaagtaca
     2521 gctacgaagc ctctctgatg gcgctgcacg accgtgacgt tatccgcacc atggcgtgtg
     2581 gtatcgctgg tctgtccgtt gctgctgact ccctgtctgc aatcaaatat gcgaaagtta
     2641 aaccgattcg tgatgaagac ggtctggcta tcgacttcga aatcgaaggc gaatacccgc
     2701 agtttggtaa caacgatccg cgtgtagatg acctggctgt tgacctggta gaacgtttca
     2761 tgaagaaaat tcagaaactg cacacctacc gtgacgctat cccgactcag tctgttctga
     2821 ccatcacttc taacgttgtg tatggtaaga aaactggtaa caccccagac ggtcgtcgtg
     2881 ctggcgcgcc gttcggaccg ggtgctaacc cgatgcacgg tcgtgaccag aaaggtgctg
     2941 tagcgtctct gacttccgtt gctaaactgc cgtttgctta cgctaaagat ggtatctcct
     3001 acaccttctc tatcgttccg aacgcactgg gtaaagacga cgaagttcgt aagaccaacc
     3061 tggctggtct gatggatggt tacttccacc acgaagcatc catcgaaggt ggtcagcacc
     3121 tgaacgttaa cgtgatgaac cgtgaaatgc tgctcgacgc gatggaaaac ccggaaaaat
     3181 atccgcagct gaccatccgt gtatctggct acgcagtacg tttcaactcg ctgactaaag
     3241 aacagcagca ggacgttatt actcgtacct tcactcaatc tatgtaatta gatttgactg
     3301 aaatcgtaca gtaaaaagcg tacaataaag gctccacgaa agtggggcct tttttagcac
     3361 gagagccttt tttgtcagct atcatctata ctttaaggtg actgccaaaa cagactcgac
     3421 gtagccttcg agctgcgcac caacacggcc tcagatgggc cacatctgga gaaacaccgc
     3481 aatgtcagtt attggtgaca ttttttgata tctaatcaca tgaaataaaa ggatttattt
     3541 ctggtcacgt ccacacattg accacatcga caaaaaaagc ccctcgactg aggggctttc
     3601 tgtttgtaat tacatccaca taatttgctg ccctgacggc aacgggtgcg gtcttacggc
     3661 gtggacttct cccggcttca caatgtatcg ctgtaccgac tcataagtga tgaacgtggc
     3721 gctgcaattc acgttctggc actggtgata acgctctttt gtcgtgtcag tgatatagcg
     3781 gcttgtacgc gcatgtgcgg catgctggca taaaggacaa tgaaacatcg cgagcacctc
     3841 ttccggtttt gttgatggtg ccattttagt taatttatcc ttataaaaca aatagataaa
     3901 taaattacat cactcatcat cttctgtttc gtactccaca tcagaaagcc tgacctcaag
     3961 ctctaaggac gtcgtgaagc cgctattatt cagaaaatgt gtcaccttag tgattgtcca
     4021 gtcctgctcg tctatgacgc gcttaaagcc tgacactctg accggcgttt ccgtataaat
     4081 atcagcccga ccagtagcca ggctgatgga gaactccgca acaccccgtt gcagtttatc
     4141 ccacttcgcc tgagcggcgc gcatggcctg cgctttcgtg gcatataccg tggtcagggc
     4201 aaaaacgttg tcagcctcac cggccatgta ttcaccttca cgcgcttccg gtactttagg
     4261 cgctttcttc tgcctgaccg gtttcgcttt cgggtgctcc agtgcgcgca ggtgtttctc
     4321 tttctttttg cgtttcagtt ttaccttctg cttttgaggc ttcgggtctt tggtgtgtag
     4381 ccattttgcc gttacaccgg tgtaggctcc acggtcagca atcgcaaaat gatggcggtc
     4441 gccgtcgctg cgggttatgg tgacctgcgg gattttttta ccgctggccg tcactccctg
     4501 ccccgctttg agaaacaaca gttttcccat ttttaccgac acctccccgc cgttgcgttc
     4561 agcaaggcgg gtcaggaatt ttgcatcaga ctcctgcgac tggtcgatgt gcgggatttt
     4621 aattccggcc agtgacggcg cgacactggc ttccagcctg ttacgggagg ctatcacctc
     4681 aacaatcgca ccgagcgtgg tgtcatgcca ggagccttca cggcgggaat tgagcgtccc
     4741 gcgaaaatct gcactccggg cgcggatggt gaccacatcc ggtgcgcccc ggtgttcaac
     4801 ctcatcaacg gtaaatttcc ctttgcatac cagggcaaaa cctttccagc cgatatacac
     4861 cgtcaggaca gcgccacgaa ccggcagccc gacctgcccg tcggcatcgt tcagttcaat
     4921 atcaagctgg tcagcctcaa agccccggtt atccgtcagg gtcatgctca tcagacggtc
     4981 gctgatattg ccggtaatat ccctgctgtc gagcatcagc atgtaatccg gcgtcagcgt
     5041 actgcctgca tcaaatgtca gcgcatccag cattatcccg cccccgtcat acccgttaat
     5101 ttagtcgcca tactgccagc cttaccgatg agcgattccg cctgtttacc gatatcgcca
     5161 taaagcgcgg ccagtgattc atcaacgcgg gtgagcgaca gcgtaaaatc aattttccgg
     5221 ggtgtgccgt ctgcaaagaa aatactccct gtttcactca ccttgctgat gacatacatg
     5281 ccgtaaatca tgccggtgcc atccagcaac ggccacgccc gaccttcctc tgccatcagc
     5341 ctgagcgtgg tcatcgtcag ctttccgccg gtcagttcgg gataaagcac accggcaagc
     5401 gtcatgtttt cctcacccac accgagaaac tgaaaggcat cccgtttacc gatacgggaa
     5461 tttgacggcc agcgataatc tgattcacgc tgcatggtct ggtgtggcaa cgtctggcgc
     5521 ataaaaacaa acatacctaa cgcgagcatc atttttcgtc acctccttaa ccgtcatgca
     5581 tcatgctggc acgggcgcgc gctcgtttat cccgctcgta tttttcgagc gcatcctgta
     5641 actggcggtc gagttgtatc cccggcgcat taccacccgt caggctgatg tgatattcgt
     5701 ttttactctg gtccacataa gagcggccag ccggtgccgt aaccggctga taagcctgat
     5761 agcctgcata agagctggtc gccggaatat aaccaccggt gccatacgtg gcggcttgag
     5821 ttctggcggc ggtctggtca agtgtgtctg actctttgtt gatgacaccg agtttttcca
     5881 gtacccagtc aataccactg cgcagtttgt tgaacgcatt aagcggcagc atcagcgcgt
     5941 cagccagtgc ctgcccgaac atgacgcccg tgtcacggca acggttcagg gtgtcctggg
     6001 tggctttgac cggggcaatc aggtttttaa accactgcca cgcggcctgt aacttttcac
     6061 ccagccagtc aaacagcggc ttaagtggcg tgaacagttc ccccaccggc gcaaatgccg
     6121 ctttcagccc ttcaaccaca ccgccaaaga atgcgctgac aggctcccag tatttacgga
     6181 taagtaacgc cccggcgaca atagcggcca ccacagccac aaccggccag ctaatcgccc
     6241 cgatggcggt cataacagca ctgccaaccg tcgtgaagat tgcccccatt gcgcctgctg
     6301 ccgcgatgat ggcattgatg ccggtgataa ccggccaggc tacgaggcca atggcaccaa
     6361 tgatgccagt cagcgccagt gcaccaccgg caataatgcc gatggttgac gccagtgatt
     6421 tgtttttctg gatccagccg tcgagtttta acacatactt tgtggccgtc tgcgtgagct
     6481 tacgcagtgc gccttcctgc tggtcaaaca ggtcagtccc caccgcctca taagcggact
     6541 gaaactcctt aaagtcaccg ccgaggttgt cctgcatgat atttaccagc tctgcggtct
     6601 ttccgtcaga ggctttaaac gcagcggtca gtttgtccag ctttccggtt gaggcggcag
     6661 tcatcagcac ggcggcggct gaactggcct cctccccgaa aatggttttc atgtattcag
     6721 cctgctgggc agtaccgagc cggtttttct caaaactggc ctgcatttct ttcagaatgg
     6781 taaatattgg ccgggtgttt cccttactgt ctgaggtttt cacaccaagc tctttcagtg
     6841 catcccatgc ttttcccgtc ggtgcctgca ggcggcttaa cacggcacgg cttcccgtcc
     6901 ccgccattga accggtaatt tttgcatcat gcagtgcacc gaccattgcg gcggtttctt
     6961 caatgctgac accggcattt tttgccacag gtgcggcata ggtcagcgca tcgctcatgc
     7021 cgtcaaaatc ggcggcggtt ttgttcatcg tcatggagag aacatccccg atatgagcga
     7081 ccttatcgtt tgaaagctga aaggcggatt tcatccccat cagcagggcg gcgttttctt
     7141 ccatcgtgcg gcggttcgcc agcgccatgt tcagcgtgac cggcgttgcc gcctgaatgg
     7201 catcaacatc cccaccggct ttcgcaatga taatctgtgc accggccgca tcatccgccg
     7261 aggcggcggt attgtcgccg agctggcgcg cctgtttgcg tagtgcggcc atttcggtgg
     7321 agtcttttgc cactccgagc acagcctgta attctgagtt tttctgcgca aactcataac
     7381 cgggcatcag cagcttaact ccggccatcg ttcccgccgc cgcaatcccc acaccggcag
     7441 cgcccactga ggccatattt ccggccagtt ccttgcctgc ctgataacgc tgtttgactg
     7501 cgttaagttt tgcctgttgc gcactgacac gcgccagcgc gtcgcgctga cggttaagtt
     7561 gtgcggtggt ttcactgata cggtttttca gtccctgctc atcatgtgca agattgcggg
     7621 tattaattcc cactgcggcc agttcccgct gctggcgttt aacggaatct gtcaggcggt
     7681 tatatttcgc ctgtaagtcc tccgccgcac gctttgcgga ttccagcact ttcgcctgag
     7741 cacgggtcgg acgttcagtg tttttaaact gtgtggcaag tgcttcggct tcctgccgtg
     7801 ccttttcaag tgcatgacca gtcacggcaa gctgtgcgct ggtcttgcga aatccctcaa
     7861 tacgggatgc gtgaccattc agctcgcgca gtgatttttg tgtttcccgg atatcccccg
     7921 acagcgactt actcgctgtg cggatggatt taaacgggcg ggatgcctgg tcaacagccc
     7981 tgagcaatac ctgtaatttt acattgttac tcattcgtgt ttccgcttcg ctggagcgcc
     8041 ttttcgcgcc atgtgatgag ttcggtcagg ctcatgggat acagttctga tggcggccag
     8101 tgaaatatca ctgccacatc cgccatcagg tcatcgaccg acagattttt cgggaacgtc
     8161 actgcaccga gttcggcgac aaaaaaccga ccaccttacc ggccagcgcc acaaggtcag
     8221 gcagttccag cgcggcgact tcctgctcgg tcagcatcgg tgccgtcatg cgcggcagca
     8281 ccttaatcag tgcatcgact tcggagtttg caaccgcagc cagactgaca ccgcgtagcg
     8341 tcccggcatt gggtttcatc agcgtgacct gttcgataac ctgctcacca cgtttgaccg
     8401 gattgtccag ggtaatgaca ttttctttgt tcatggtttt ctcacttctg aatcagggtt
     8461 aaccggccag cctggctgac cggatgaaaa tcacaggccg atattgcggc ggtgttgctc
     8521 cagccggtcg acgccgttca ccttctcaat catgttgatg gtgtcgattt cgaccagttc
     8581 cttaccgtcc atcgtcagcc ggaaataggt gcagaccacg gagattttcg actcggtatc
     8641 ttctccctgc ttcccctcgc cggtgtcgat ttctttctga cgtccacgca tgaccacttc
     8701 gacggccacc gtttcgccgg tatcgtcgcg ctggtaagaa ccggcaaaac gaatcggcac
     8761 ggcatccacg ccggttgcgg cgtaaagctc ccagataacc gaatccggga agccaccgag
     8821 cgaccactcc attgacagcg catcgtcatc aaggccgagg tctaccggtg cgctgccgtt
     8881 catccccgca ccgcgatagt tttcgagctt acgggtcagt tttggcagcg tgatggactt
     8941 tgcaacgccc tgatagctgt agccgttcag aaagacgttc attaacttga gtttgcgcgg
     9001 cattgccatc ggtcaggctc cttaattgct gttaaccgaa gtgaccagac ttgccaggta
     9061 tctggaagta atacgctggc gcagggtcag attttcaaga ggaggcaccg gggtatagtc
     9121 gtagtcgata tacagttttc cggccttgag ggtttccgca tcgttggatt cttcgctgaa
     9181 ccagcatgtg ccatccacga tataaccgtt gtttttcagt tcacggaatt tggcattgat
     9241 gccgtcaatg atgtcgcgaa tcagcgttgc ggtgatgggc ttatcaatag cccacatgtg
     9301 cgcctcagcc atcgtgtcgg ccagtacctg cgcggtgcgg gtgtaacttt caaagaggaa
     9361 cagcggatca tcagagcagg tacggttacc ccagaagcgg aaaccgtcgc ggcgaatcag
     9421 cgtagtgacg cctgactcat taagcaggtc agcatcagtg ccgggcttct gcaaatccca
     9481 gaagacggat gcactgatgc cggtaacacc gttcaccccg acgttggaca gcgttttatg
     9541 ccagccctgc tcctggtcga ttttagcgcg cagccccagc gcacgggcgg tggcatacgc
     9601 ggtagcggtg gtactggtaa ccgtatccca tgcgaggaaa tctggccaga tgaccatcag
     9661 ctcacgctgg ctgaaattct ggcggtaggc tttcacctcg gaaatggttt tacagcccca
     9721 tgcgctgata tacccgaatg cattcagttc ctggcttacc gatgccagtg cgacggcaac
     9781 ctctttggtg tccagccccg gcacaccgag aatgcgcggt ttaacgccgg taaccgactc
     9841 agcccccatc agggctttca gtccggtgta ctgaccgttt tcgtcggtgg tgccgatgat
     9901 attggaaacg gtctgcgcga gcttcgtttc ctcgtcgtcg ccggtgccgt cttccacacg
     9961 cacaacaacg gtgaccggtt ttgactggtc agcgatggcc tgcaacgatg ccgccagcgt
    10021 gcctttttta ccggcctttg caattgcgct ctgcacattg gtaatcagca caggtttatt
    10081 gagggggaag gtttccgcat ccgcatcgct ggccgtgcag accatgccga caatggcggt
    10141 ggatacggtg gaaatgacgc gggtgccgtc gttaatctcc agcacctgca cgccatgatg
    10201 atagtcgctc atccgtttaa ctccgtggtt aatgggtgca actattttct gttgggcagt
    10261 gcatgagacg ctatttgacc tggctggtca gtggatgaaa caacagataa agaaaaggcg
    10321 ggcaattcac ccgcctgtcc tgatttgtac tcactcattt tccgactgac aatttactta
    10381 gcccaaaagc tatcaaatct gacagtctgc ttcagacaaa gagcggtcat taaatgatag
    10441 aagattttat ttcattttgg tgccagaagc tgccgctcag taactcccgc aataaatggt
    10501 gcgaagaatg agcgtctggc gaccgaattt cattaccttg ttatatgcca ttttcaagat
    10561 ctgcgattag gtcaaagcat agcgatttat aatcctttac gatcttcggc cgaatggtta
    10621 ttgaatgata tttcagcaac gcaagggcga atgaggaaaa atacagccag aaccagtcat
    10681 cttccggtaa attgacatct tgcacagtcg ccgaccaacg cccgtaaatg ggaggatgag
    10741 tctctgaacc cgcgagacgg tttcttcctg ttgcgaaaag gataaaggcg aatgctagag
    10801 cgatccccat catacgcttc atcctaacct tttccggggg atacccactg cttgcggcgt
    10861 actcatcgat cttggatatc atcatatcct tagcgaactg atcacacaga tattcttcgt
    10921 ccttcgggtc ttccggtgca tttccttcaa ctgagaaaat tacgtgcttc agttcatgta
    10981 aaaatacata tgccgtagcc atgcacataa gatcatatac tgcagcgctc tctgaatctg
    11041 acggcctacc cttctcaggc tcaggaatag ccactggcca ttcaaagtcg tcttcatgaa
    11101 atgcagtgct caggtctttc actgcatcaa gtatcgactt gaaggcttca tcttctgctg
    11161 cctgatctgg aatcttgttg atttcgttta tgtcgagagt ggcaccgcat gatttgaaca
    11221 attcaatcaa cgaacaataa caatgtaacg cctgcattcc cgcataactg aaaagccata
    11281 tttgacgcat tgatctgctg gtgtactgga ttgcggagaa tcctccagca tctaagttaa
    11341 atccttcgcg gtcaccaatc agtcggaact gggcatcata atcgttagcg atagattcaa
    11401 tctctgatgc tctctcagga acaacaccgt caaaaagttt tcgaacattg tctgtcgttt
    11461 ccactagtcc tcctatggca tataaaagtg cgttataccg gccccgcaag gtcggcttcc
    11521 ttgcaagcca gattaccttg taaggtcaga aagtccaatg acaactttat gtacgaagca
    11581 gtaattgagc tactacgtat aaatgaccgc tcctggcaca gagcggcctc ccagattcgg
    11641 ctttactctg tgccatagat atgtaagctc ccaccagagc tcatacaact tattgcggca
    11701 tttccggcca ttcaggattt gcaggatcca cacgactgac cagaacgctg tagcgttccc
    11761 atgttttaag tcggctacgc tcctcatctg ttgccatatt cagcctgaca gcacgctcca
    11821 gcggcaaaat cacggattca gcatctgcaa gaagtctggc tttccgattt tctgcctgct
    11881 gctgcaattc ctctgccgta taaatgcgtt taatcacttt gccgtcctta aacatccagt
    11941 tccctgaaat gtccgcccgt cggttagcag taatatccgc cacttcaaca acacttaatc
    12001 catccggtct gatagctgtc acatcctttt ccacatagcg gatgatatta tccttgtcgt
    12061 acgctatttt tatcgtgtca tcagcaaaat acttttgttc ttcgtaccag ttcttaccat
    12121 cttctgaaaa aaaccagaca acatcaaaat cccttgttaa ttgatattgt tcaaccgttt
    12181 ttggattacc tgccgttata tttatcaaat gctgcataaa ttatacctgc gccacgttat
    12241 accaagtccc gttaatgtat ttctgcaccg gtctgtaata tacgccacca atgttatcgg
    12301 cagagtttga gccggtatcc tgaacaataa tgccggaata tacacacccg gacggtgcct
    12361 gatgtgtcca tgtcatgcca ttgttcgcag gtttgtatgt ggcagcacca ccaagccgaa
    12421 tatcccggac atagcgtgaa tcaaagttgc catagttaga tggtgatacc tgcccgttaa
    12481 cagcaaaagt gatgctgtta tctgtatttc tctgactgta aaaatgccag cctgcatcat
    12541 cacctaattc agccaccacc ggacgacttg agtttcccca caaattgaat gcggcttcct
    12601 tcgtggatgt attactgctg ctgaccgtga attttttccc gctaccggca cgtactttgg
    12661 tacttgagac aatatcacca gtaacactca ggccatgccc cattgataca gcacccgtgg
    12721 catcatttat aatcagtggt cttaaattat tataagtgcc taatctgtca cctgatttag
    12781 tcaacataaa ataagtgctg ctgccatcat tcctgataaa gaatccataa ttgccataag
    12841 caatgcgcag accattagca ctgcttgatg taatctcacc tcttgaacgg agaccataag
    12901 cggagctgag tgataattct tcctgagcat cagtattacc agtcgcccag cgaacaaccc
    12961 cgccctgtac tgtttcatgc cagatagtgt ctccttctcc accacgaaac tttctgagat
    13021 attttttgcc gcctctggtg cctgaacata aggccgtaga catataggta ttctggcttc
    13081 cgccgtcctg attaatcgtt ccggtcattg catcgccctg acgattccag tcacgacgcc
    13141 agccggggga gtagccgtcc ccatgattaa tgtaagtgaa ttgcgcgctg gttgtaccgc
    13201 caccacttga tgttgtcggc gttgtcactc ggatggtgat tgcagatttt gttcccatga
    13261 cctcgacgac acaaccagcc aggtggatat caccacatcc ggtatccgta atgattttgt
    13321 tatttgcata tgaccaggag cctttgcaca tccagtacgg atgattaaat gcaccacggg
    13381 aatccagcca ttcaataaac tgagcggttg tccagtttcc ggcttcagtg ctcaaagcgc
    13441 cgctataagc acgacaggca ccgatatttt tcgtgaaggt atcctttccc ggaatatccg
    13501 caccgttctg atctttctga agacgttttt cagcattgtc ataggcagac ttcactgctt
    13561 ttggcgttgc cgcgagggtt tcagaatcac tgttggtggc gctactgagc tggacaagac
    13621 cttttcgcgc cgtggtggca tcctgtgcag tgtatttccc gttagcaagg tcatacgctg
    13681 tcttaaccgc ctttggtgtt gccgcaagcg tttcagaatc gctgttggtg acgctactga
    13741 gctggacaag accttttcgc gctgtggtgg cgtcctgtgc ggtatatttc ccgttagcaa
    13801 ggtcatacgc ggcctttacc gctttcggcg ttgcggcgag cgtttcagac gtgctgttag
    13861 tcgcactact gagctgaaca aggccttttc gcgcggtggt ggcatcctgc gcggtatatt
    13921 tcccgttagc aaggtcatag gcggtcttta ccgctttcgg cgttgcagcc agtgtttcag
    13981 acgtgctgtt ggtcgcactg cttaactgag taaaaccttt tgcggtcagc gaggcgtcag
    14041 ggtgacgtcg tgactgttca tgttctgcaa ttttgtcatc aacgtaatcc tgcgttgcca
    14101 tcaccgttgt ggtgtcaatg gtcagctcca ctgaggccac actgctgacg atgatgacca
    14161 tgcggcaggt ctgcgaacgc cctgagcctt cggcaagggc tggcttataa ctttcggcca
    14221 tgttcgccac ggcaattagc gttcccgcat catcgtacag gccaagttca cgcatccaga
    14281 aaccgcccac ctccggcgga ataaccagct ctgcgataat ataattactg tttcgtttgt
    14341 cctggctgat tttgttcagc gcatgtcgcc agacttcgtg gataagcccg gtctgtccgg
    14401 catccgggac aggcaattta ccaccgccat ccccgacggc catcgtggta atgttgacct
    14461 tccgccctcc cggtgcggtt gccgctgcca gctttgctgc accggcagtg gtgataacgg
    14521 ttctgaattt tgtgctcatt attcctcact tatccggggt aaaccgtaat tacatcgccg
    14581 tcgtaagcca caccaccggc gaacaggtag ccgggaatgt cccgggtaat gttcaggcca
    14641 ataagatggc ggcttgcagg tttggcatca gcaatcagcc gttccatttc ctgatacatt
    14701 gcctctgtga tgccgctttc cagtacacca atatcaagcc ggaaggtgcc gggcgggtca
    14761 ctgttttccc accactccgt cacgttaatg agatagccga gtggctccac cacgcgtcgg
    14821 attgcgccta tagtgccttt atgacagtgg atgaaatagg catcgcgaat aacggcgcgt
    14881 ttggtcgctt ccggccactt ttcatcccat ctgtcgaccg aaaacgccca cgccagccac
    14941 ggcagcagat ttgccggaca ggtgtccggg ttccacagct cacgaatact gaccggcgtt
    15001 ttttcaattt ccgcacaggc ttttgcggca gcaatttcaa gcggtgatga gccggtcggc
    15061 agcaggcgcg aatcactcat ccgagcctcc ggtcacgacg cggtattcgg tacagaaaga
    15121 cgcctgcgta ctgttgagca cgatgtcggc cagcggtgca gccagttcga cacgctgcac
    15181 gccttccaca tgcaaagcgg cataaatggc agacagacgg atgtcgcgcc ccagccggtg
    15241 ctgtgccgtg atgtatgctt ccagtttttt cacggcggcc gcgcggatgg gttcactttc
    15301 gggaccaggg taaaggtaaa gcgtggcgtt tatctggtat tcaacaatgg cggcagactg
    15361 cacggtcacg cggtcggcta ccggcctgac gtcctcgcca ttaagggcat tacgcaccac
    15421 ggccagcagg tcttcggatg cgacaccgtt attttcacgt gacagcacgg agatagtgac
    15481 gcaggccgga gacggactgg tgacagagat atccgcgaca cgcccgtcag cactgcgtcc
    15541 atgatactga taggcaccca ccgacccggc gacgcttaaa ccttcaaacg cctgctgaat
    15601 acgcagacga taatcggtgt cagactccat cactgccggt gtcggcggga tggtcgaatc
    15661 atctgccggg gtgatagtca ggcgcgtggt gttgtaattg gcaccaatca catcaaggtc
    15721 attaccggcg gcacaggcca gcatcaccgc ccgcgcagcc tcattcacac gctgacgcca
    15781 gataagctca cgataagcat tttcctccag cagtttgacg agaggctcag attccagtgt
    15841 cagggtacgg gcgaccgcct cctgctggtc ttccgggtaa agggaaatca gtgtcgcctt
    15901 gcgttcggca agaatggttt caaagtccag cgcctcgacc acatccggtg cgggtagctg
    15961 gttcaggtcg ataatcggca tggtttcaac tcacagggat ggttaatgaa agtggctggc
    16021 cggtgtcgtt gtgctggccg gttaacgtga ccgtcattcg cccgtcaaaa ctgcgctcag
    16081 tggtgacgga tgacagggtg acgcggggtt cccatttcag cactgccatg taacaggcga
    16141 ccttaatctg caactcaagc gccggagtct gcggctggtc aatcattgac gccagcaacg
    16201 agccgtaatc acgacgcatc acccgtgagc cgaccggtgt gcgcaggata tcgccgatac
    16261 tctggctgat atgctcaagg tcagtgacag tcaggccatc actgcgattc attccgagat
    16321 aacgcgctgt cattttgtcc cctgtgtcca gttgtctcct gacttaacac caccgtgacc
    16381 gtggtcatcc acctgaacgc cgttagaagt gaatttcccg tcggtatgct cgatgttgcc
    16441 gtgcatcttc ccgcccttct gcacttccag cgtgccggta gtcagcttgt tggtgcagac
    16501 cacctccggt gtgtctaggg tgatgcgagt tgacgctttc accatgacca ccggcaccgt
    16561 ggcggtaaca gaatcagaag ccgtcacgct ggccgtttta attccgcttg ccgtgagcgc
    16621 actggtttcg ggttcatact caatcaccgc cccgtcaggg aaacggatgt acagggcatc
    16681 cgccgacgca gacggcgcgg ggttatcacc ggaataaatc cccggcagaa caaacgccgt
    16741 gtcgagttcg ccgcccacgg acagaatcag cacctgctcc cccaccgaag gtgcccacca
    16801 tgtgcgtgaa cgtccggcac gatgggtcag ccactgaagc cagtcagtgc acatgccgcc
    16861 ggtctgcaca cggcagcgac cggctttaag gttggtttcg acgacaaggc cagtacgaat
    16921 catgttgcgc agtgcgcgcg cgagttcctg aatatttgcg agagtgttca tgcatgtgag
    16981 attgcacaat atataaaagt tatgctatct ggattcattt gtagaaccac cagacaacat
    17041 tcaaggagag cgtaatggtc agctataatg tgactaatgt gtgggagcta atcgtcttgt
    17101 atcttttggt ctttgtagta ttagcatttt ttagctttgg taaaagtaac cttatgaggc
    17161 ttattgcaca ttatttcaaa tttggatatt cagataaaaa attaaaaaga ctggaccgcg
    17221 agtggcgcga cattcaacta tttaaaataa ttaacggaat caatgtatca ggcatcaaag
    17281 atgtgaaaat gatacagcag gggttgattg atggaaaact aaaaacatcg gattttttcc
    17341 ttactcgctt ctggggagac ataacagaac cgccatgcat cacaaaaaca atgagtgcag
    17401 tcctggccgg aattctttat attaccttcg catgttgcat acacaacgag caatctgcca
    17461 tagtagagga tgccatagcc ataccatata aaaatatgat gtaccatgtt tataatgaca
    17521 aggttctttt gttctccgac aataaaacag ataagttcta taaagctttt agcattgccg
    17581 attgcaagag actgcagaac acttttgtaa cagacacact tcctgcaatc gcctgcaata
    17641 agctcttaca gctaaacgag gaggactccg aatggttaag tcaggagatt aaagataata
    17701 acagccacag aaaaacatta ttaataatat ccctcgtcta tttcacttca ggtctggtta
    17761 tacttctgtc atatacaaaa ttcctttacg ccaataaaaa agttgtagaa tacaaatcat
    17821 caaataaaaa ccactcataa tcctctaaac attgagcgac cagcacagcc gctcaatgtt
    17881 taattgcgca tcagcctctg cctggataaa actaacgctc aaggtgagcc aggataatct
    17941 cttcaatcat ctgcacatcc tcaccggtaa agccgagcag aggacgcgcc ggataatcaa
    18001 ttttcttacc gtctttccgg gtttcttccg acagaccgaa ctgatgcaca ctggcgattt
    18061 tcggtgactt cccgccgtaa aactccattg atgcctgttc cgggctggcg cggatatgca
    18121 aaaaacgact ggtgataagt ttcgaaaaca tttttcgctt aacacgacca gtcttttttc
    18181 tggcgctctg ctgctggcgt ggcgcgtagg gtgtgccgtc cggggctttc tgtgccatca
    18241 cccgacgctg ctgactctgc cgcagacgct tcgccagctc tgcactcagc cgccgacgcc
    18301 ctgacggtga cagcggctca ataagtccgg tcagccggtc ttcaaaacgc ttaaactcat
    18361 tcatcccact tgctcaccag ttcgccattg atataaagct ccatcgggcg ggtgaccggt
    18421 tccggcggcg ggggttccgg gatattcttc acatgcagcg caccgtcaac ctcactgacc
    18481 agcgtgcgct cggtcagcat caggctgatg ctgatatcaa agctgctgtc attgttgatg
    18541 tctgcataaa acgtgaagcc ctttttctgg ccttcgtcag tggtcatgat gtcgggctga
    18601 ttttcccgca gccacgccag caccggcaca atgagcaggt caaaatcacc ggtaaagtcg
    18661 gtcacaatga cattgagcgt gtaacgcttt tcgaatgaca acgacgccgc cagtgtggag
    18721 gcaatactcc cgttatccac gaatatccgc agcatatcgg ggttagtttt cagcaccgtg
    18781 acggcatcag tcagcgccct gcgcaggctg tcgggtttga gcatcgtttt cgtcctgaca
    18841 gtgtttaatc atttttacct ggctggcaca gcgtgccagc gcgttctcaa gctgccggat
    18901 atcggcactt aaatcgccgt tcgtctccgg gtcactgccc ggcatcgggc aaaggcccac
    18961 tttcgggcag gcgttgtgga caatcactgg cgtcggtgca ggccgggcgc tggtgcaacc
    19021 ggcgcacagc atcaggcagg ccagcgccgt accagcggcg aaaatcttcg ttttcattga
    19081 gtaacctcgt gatggttttc tcgcgctgtg cttcacgctt cgcggcgttt tccagttcct
    19141 gacgcagtgc cacctgcgcc agctcgtttt tgtctgccct ggtgagtgca acatgaagct
    19201 ggtttttcag catggtgatg gtcgtctgct gttcactggc gacgttgttc accctgtcca
    19261 gcgaggcgcg caggctggcg ttttcatgct tcgccagaaa cagccccgcc accgccagcg
    19321 ataacaacac aaccagcaca atcatcagct ttgacatggt tcccgcccct caagacgttg
    19381 acggcaggcc gtacgtatca gccggaagaa caccgacgcc acgagataaa tcagcgcggt
    19441 aaaaatccac ccggcagcga ccagcgagat aaatgtcgcc accatcacca ccagagccgc
    19501 cgcccgtctg caccacggca ccggctgcaa aaacagcgac atgacaatct tcacggccag
    19561 cgactccggt ggcagctccc acccgtagcg ttccagcgca tactcagtgg cataaacgcc
    19621 gacaccaccg gcaaccacac agataaccgt cgccagaatc gcccaggcag cgacaaaact
    19681 gacggccacg ctctgcgggt aaatcaggga cagtgccagc atcagcgcca gcgacacgtg
    19741 cagcatcagt gaaagggata atttcttcat ggtgtttact ccgtttaagc cggtacgccg
    19801 ccagcggtac gccagacggt gaccagtttt tccagtgaat gctcacgctg accgtaaccg
    19861 gcacccggca gggacgccca gatattgcga cagcgtgaaa tggcgcgctc aatgcgtccc
    19921 gcccggatgt catccagtgc accgcgttcg cggatcaact gaatggcgag tctgtcctgc
    19981 gacaacggac tgaaatccgg cagggcaagc tgtttgcggt agtgcggcca gaacaggtaa
    20041 agctgctgat agcgaccgga ggccgtggat ttttcaccgc gacggttaaa caccttcgcc
    20101 ggtcggccat gtgcgaacgg gtggtcactg tagtcggtga agatttccgg cttcccgtcc
    20161 agtccggtga ctatcacgtc atagccccgg tttttcgtca gcgggtgatt cgccgtccct
    20221 tcggacacgg ccagcatgtc gagaaaggca gcgatattct gatgcgtgtt aattaccggc
    20281 attactgttt ccccctgccc ttaaagcgac gctgaatggc aatctcaatc acctgataac
    20341 cggcgatacc cagcatggag ccgatgccgc acaccgcagg cagtgacagg tcaggaaact
    20401 gcaccagaac aacaccggca accatcgaga caaaaccacc gagcaacatg cgcccgataa
    20461 acagacgcgg ggtgatgggt tcaccaccgg caagcacctt gccgacaaca atcagcaccc
    20521 caatcatgaa aagcgacagg acgctttttt cttctgctgt catgcgttac tcccacagat
    20581 tgacagtttc agccacgggc gcggtctgaa cgtcgggcag ttcgacggcg gtgccgtgcg
    20641 gcagcaccgc acccagttca accagtcccg gatttgcggc gagcacggcc tcaaccacgc
    20701 cctcagtgcg cccgtaatac cggacacaaa tggcgtcgag cgtgtcgccc tgtagcgcaa
    20761 aggtcttcat cagatttgac tcacgatgca gcgcggcttg tcctggatac gcgccaccgc
    20821 ccagcgcata tcccgccaca gttcatcaat ggtgctgtca atgctgtcag ccttcttgtc
    20881 gcctttcgca ctggcatcca cgccgcgata acgctcataa agcgacgcgg tcgccatcgc
    20941 acacacggcg cgctcgtagt aaaaaacttt gatactttca ccgtcgatgt cgtccgccgg
    21001 aacgtccgcc agacgcgtaa aaccggcggc aattttctgt tcgcggtact cgtacagctc
    21061 cgcatttgtt tcagccatgc ctgacttgat ggcctcacgc agacgggcgg gggcgacggt
    21121 ctgctcaagg cgcatacgtt cccggacgcg cttcgggtcg atatcgggaa aaaagaacgt
    21181 gtttttaatc accggctcgt cgcctgccgg ttgcgggatg accaccgtac cctcaccgga
    21241 cacgggagcc tcctttcgcg gaataatcag cgtcatcatg actacctctg aaaagtcggg
    21301 cggtggacgc cggtgcagtg tcaggtgatt caccctcact gaccggcgtg ccgccctggc
    21361 gcggggcgca ttcggttgtt aactggcttt ctttttcggg cgtccacgtt ttgccggtgt
    21421 cacgctccgg gtcttacgcg gggtacgggt ggccgctttg ggctgcggct ccggcttcgg
    21481 tttcagctcc cgctccagtc gttcaatctc ttttttgacg cctgcctgac agtcgagctg
    21541 tgtcgcacgt tgcaggtgag ccagcgcacc ggcggcatca ccaccgtcac gcagaaacag
    21601 accggtgatt ttgtgcagct ttgcgcgcac ttcatcaggc atgtcagccg tggcggtcag
    21661 ttcaagggtc tccgtcagca ggcgggtatc cacagactca ccggcagcgt gagcgcgcat
    21721 ggccgcgagc gccacctcct cggtgaacat gtacggcggg gtgcggcggt gtttacccgg
    21781 catggtcaga ccgtacttca gggcataacg ggcaatctcc agcgcaccgg caatatcgcc
    21841 ggtatccaga cgccacagca tgaccgtcat cagaatgtca tcctgtgcac ctttgccctg
    21901 ctccagcacg ccgttcaccc acggcaacca gaacggcagc agttcgcgct ttttcgcggc
    21961 cttaagctct tttgaataaa tcgctttcag tgtgcgctgg tctgcggcga gcttaaccag
    22021 catctgctca tagacagttg catgtcgcag cggggcggct tcccgctgcg cggtcatcgc
    22081 tgccgagacc cgcatcatgt ggcgctgtgc gggactcgtc atcggttacg ctcccggctc
    22141 tgcggtcgcc ttagccggtg tggaaaaatc accgacctta attttttcca ccagacaacc
    22201 ggcggcgtag tcttccacca cgtaatcaat gttcattgac tcgtagttct ccacacggtc
    22261 gagtttcggg ttttcctcaa tcacgcggcg atggctgtca tccatgtagt agatggacag
    22321 gttttccagc ttcgtgatga gcatcgcatc cgccgggaag tacgggacgc gtaccgccgg
    22381 caggttaccg atgcgtttct ggctgatgat gacgtctgcg gccagcattt cgctgttgtc
    22441 ctgctccctg ttgacgatgg ggaaatactt gtccgccagt agctgacgtc ccacaatcac
    22501 cacaaggtca gggtcttcct gataccacgg ctcaatcaga ttattggtcg catccatcac
    22561 cagtgcgtca aggctggcat aatcaccgcc cttgcccacg cggatgacct cagaggtcgt
    22621 gtgcccttcc tcgtcagtga ccttgctcat cacgcgcgcc ggagcttcat tgcggtattt
    22681 ctgcagccag ccgaccgcca catcctgtag catcggatta ctgctgcggt cagaggtttc
    22741 ggcacgcttc acgccgttaa aaccggccat gatgaaatca agggactggc gtttgataat
    22801 ggcgttacgg atacggagct ggaaatcctg ataacgcgcc cacaggtcca gcgttttgta
    22861 gcggatataa aaatcgaagt taatctggtc gcattcgtac ttgttagacg ccagcttcga
    22921 gaagtccttc ggctgacgct cggtgccacc ggcggtgtcg gtggtgctgg cgatggagcc
    22981 ggtgacaccg atgccaattt tttccccttt catttcgctg accggcacaa tgttgatgcg
    23041 ggtcagaaag tcagaggact cctgcatggt gttcatcagg gtctgggtga ccgacggttc
    23101 aacggtgaat tttttcgaca catcaccggc gtcgatgccg ttcagttcgg caacacggga
    23161 caggtaagca ttaaatttaa agcgggtttc ctggcgcata gtttttcctg aaattaaggg
    23221 ttaatcgtga aggttttccc ggactgactg acgccggtca gcagttcgtc agcagggcat
    23281 caccgctacc gccagtggcc ttgctgcggc gctgctgggt cagactttcg gtgtggtcga
    23341 gactgttttt caggcgggtg aatgcctggc tggtttcatc cgccctgtca gtcacatcct
    23401 gcttaagtgc ggaaaaggcg gtttccatct cagcgaggcg ctgctcagtg gcgctcagtt
    23461 tttcctgcac atgttcagca acagcggtca ccgcttcatg cacatcattc agacgggcgt
    23521 catcgctggc ctgtttgcgg ccaaaaatgg atttcacctt ttcggtcagg gcggtgaaca
    23581 cggtttcagg caggtcttca aattccagct caacgggcgt tgccactgaa atcaggtttt
    23641 cagggcttaa tttgaagcgg ttcagggggt tgtgttttgc cgtgcggcag aattccaggt
    23701 attccgtgcc gaggcttgcc gggtcatcgg tgacggccag ccccaccaga taacatttgc
    23761 cggtattggc aaagttcggc tgaatttcca ttgaggtgta gaccttctgc gcggccttgt
    23821 tcatcgcgat aaggtcatcg gtcggggtga ttttcgcaaa cagcgcccat ttgcctttca
    23881 gcgccgaatc atcgtcaatc ttttcggcct tcagttcggc cacatcgcca taacgcttaa
    23941 aaataccgtc aggcaggatg ccgcgcagat gttccaggtt aatgcggcaa ccatagactc
    24001 gcgggtcaaa ggtttcggcc atttcctgaa tatcctgcgc actgatgaca cgcccgtcac
    24061 aggtgtcacc ctcaacgccg atacgaaaga attttgagac tttttttgcc attgtcagga
    24121 gtcctgaata gtgattagag gagtcacatg tcggcatcag tttcccgacg atgcgcatcc
    24181 tccgccatca gtcccggatg gcttatcact gacacaacag caccttagcg aatcgcgggg
    24241 cgcgactcag tagccttgcc gtgtattcat cacggcgagg tattcatgac catcaccaca
    24301 gacaccactc ttttacacga cccgcgtcgt caggcggcgc tgctgtactg gcaggggttt
    24361 tccgtgccgc agattgccgc catgttgcag atgaaacgcc cgacggtgca gagctggaaa
    24421 cagcgcgacg gctgggacag cgttgccccc atcagccgtg tcgaaatgag cctggaagcg
    24481 cggctgaccc agctcatcat caaaccgcag aaaaccggca gtgacttcaa ggaaattgac
    24541 ctgctgggac gccagattga acgactggca cgggtcaacc gctacagcca gaccggcaac
    24601 gaggcagacc ttaatccgaa cgtcgctaac cgcaacaaag gcgggcggcg caaaccgaaa
    24661 aagaattttt tcagtgacga ggccatcgaa aagctggagc agattttctt tgagcagtct
    24721 ttcgactatc agttgcactg gtatcgcgcc gggcttgagc accgcatccg cgatatcctg
    24781 aaatcccgcc agattggcgc aacgttttat ttttcccgcg aggcgctgct gcgcgccctg
    24841 aaaaccggcc ataaccagat ttttctgtca gccagtaaaa cgcaggcgta tgtgttccgc
    24901 gaatacatca tcgcctttgc ccggctggtt gacgttgacc tgaccggtga cccgattgtc
    24961 ctgggcaata acggcgcaaa actgattttt ctcggtacca actccaacac cgcgcagagc
    25021 cataacggcg acctgtacgt cgacgagatt ttctggatcc cgaattttca ggtactgcga
    25081 aaggtggcat caggtatggc ctcacagagc cacctgcgct cgacctattt ctccaccccg
    25141 tccacgctgg cgcacgacgc ctacccgttc tggtcgggtg aactgttcaa ccggggacgc
    25201 gccagcgccg ccgaacgcgt ggaaatcgac gtcagtcata acgcccttgc cggggggctt
    25261 ctctgtgcgg acggccagtg gcggcagatt gtcaccattg aggacgccct gaaaggtggc
    25321 tgcacgctgt tcgacattga gcagctcaaa cgtgaaaaca gcgccgacga ttttaaaaac
    25381 ctgttcatgt gtgaatttgt tgacgacaag gcgtcggtgt tcccgttcga ggagctgcaa
    25441 cgctgcatgg tcgacacgct ggaagaatgg gaagactatg caccgtttgc cgcaaatccg
    25501 ttcggctccc gaccggtatg gattggttac gacccgtcac accgtggcga cagcgccgga
    25561 tgcgtggtac tggcaccgcc ggtggtggcc ggtggcaaat tcagaatact tgagcgtcac
    25621 cagtggaaag gtatggactt tgccacccag gcggaatcca tccgcaaact caccgaaaaa
    25681 tacaacgtcg aatacatcgg tattgatgcc accggcctcg gtgtcggcgt gttccagctt
    25741 gtgcgctcgt tctatcccgc cgcgcgcgac atccgctaca cgccggaaat gaaaaccgca
    25801 atggtgctca aggcaaaaga cgttatccgc cgtggctgtc tggaatatga cgtcagcgcc
    25861 accgacatca ccagctcgtt tatggctatc cgcaagacca tgaccagcag cggacgcagc
    25921 gccacctatg aggccagccg cagcgaggaa gccagccacg ccgacctcgc ctgggcgacc
    25981 atgcacgccc tgttaaatga gccactcacc gccggtatca gcactccgct gacatccacc
    26041 attctggagt tttactgatg agcaagaaaa aagggaaaac accgcaacct gcggcaaaaa
    26101 aaatgaccgc cagcgccccg aaaatggagg cattcacctt tggtgagccg gtgccggtac
    26161 tcgaccgccg tgacattctg gattacgtcg agtgcatcag taacggcaga tggtatgagc
    26221 caccggtcag ctttaccggt ctggcaaaaa gcctgcgtgc tgccgtgcat cacagctcac
    26281 cgatttacgt caaacgtaat attctggcct cgacatttat cccgcatccg tggctttcac
    26341 aacaggattt cagccgcttt gtgctggatt ttctggtgtt cggtaatgcg tttctggaaa
    26401 agcgttacag caccaccggt aaggtcatca gactggaaac ctcaccggca aaatataccc
    26461 gccgtggcgt ggaagaggat gtttactggt gggtgccgtc cttcaacgag ccgacagcct
    26521 tcgcgcccgg ctccgtgttt cacctgctgg agccggatat taatcaggag ttgtacggcc
    26581 tgccggaata tctcagcgcc cttaactctg cctggctgaa tgagtcggcc acgctgttcc
    26641 gccgcaagta ttacgaaaac ggcgcacatg ccggatacat catgtacgtc accgatgccg
    26701 tgcaggatcg caacgatatc gaaatgcttc gcgaaaacat ggtgaagtcg aaaggccgca
    26761 acaactttaa aaacctgttt ctctatgccc cgcaggggaa agctgacggc attaaaatta
    26821 tccccctcag tgaagtggcg acgaaagacg atttttttaa tatcaaaaaa gccagcgccg
    26881 ctgacctgct ggacgcgcac cgcatcccct ttcagttgat gggaggcaag ccggagaacg
    26941 tcgggtcgct gggagatatt gagaaagtgg caaaggtctt tgtccgcaat gagcttatcc
    27001 cgttacagga caggatccgc gagataaacg gctggctcgg tcaggaggtc atccgcttta
    27061 aaaactactc actggacact gacaacggct gaacatcgcc gcctgcgggc ggctttttta
    27121 caccccgtca tcacgccctc acacgctcac caccgcacaa aacaccccgc agacacacca
    27181 gcgcctcaac gggcagacta agcgccgtca cgacgcgctg agacgctgaa aaaataaaat
    27241 cagcaccacc gccagcgcgc agtgctttcc ccgcctcgcc cgcccgcttc atggggcgat
    27301 tttgatgcag gtgcatgagc tctctcagac tgaacaaaag ctagcaacgc ccaaacaatc
    27361 acgatgtcca aaacgcatgc attttcatgc aaggaagagg attatgtcga tacgttaaaa
    27421 aactaccaaa gtaaattata agcatacact aacaactcaa aaaaatgtcc tcctcacaaa
    27481 tcattatgct tcctctgtat tcataagtct taaaagaatt tctccagtgc tagcatcaga
    27541 cattagatca tttaacgctt tgattaacgt ttctttatct ttgcaactaa catcccgttt
    27601 gttataagaa atcacacatg gataaccatc tgattcattt atcttccaaa cagcaatgtt
    27661 ctctttaact tctgtttgct ctctcttagt tacgaacgat agagcttcgt aatcgatgta
    27721 agggttgatg tttgtaaacg agctcacaac ggcagtaagc gctgtctgat taggtgcgag
    27781 ttttttttca ctcgtaactt tcaaaaacac tcgcccttga ctaagctcct ttacactgtc
    27841 atttaagtca ctgataacac taaaaatttc ttctttattt gatcttgctt ttttggctgc
    27901 ctctaacccc acagctaatg aatttttgaa atccattttt ttaacctctc gtaattacag
    27961 cgataacagg taaatgatca aagttagttt ttggacttgt caccaaattt ttaaagactt
    28021 catcgttaat gattttaacc tcatcctcca aaagattcca atctccacca gaaatgaatg
    28081 aagaggagaa aataatttga tcaaatgtat gccaacgggt tacagtaccg ccactgtaat
    28141 aatatgtccc gtaagtactc aaatgatgat cttctgatgg aaaactaatt gaagccccca
    28201 taaaattcca aaaagggttg tataaaaaaa tatctttttt gacaaccaag tctctatccc
    28261 ttgttgcccc taaagcatgt gtaatagatt tattaaaggg atcgtcattg taatcaccta
    28321 aaagaataaa atactttcct gacttttcct ctatacatct actgattgct cctcgcaaag
    28381 cttttcctaa ttcatatcgt tttggcattt cttcattaga atcattacct ctcataggcc
    28441 aatgcgatat gtagaaaaaa aaatgatccc cactattatt atgcatgagc tgaacttctt
    28501 gcccggctct taccgctcct aacaatgact gttcaacaat acttttacta tcaatgtaag
    28561 tgaaagtagc tagctttata agaatgcaca tatcatgttt tatgcttcct tcagtgaaag
    28621 tcccatcata tattatgaac cctttctctg agaaaatctc ctctaaaacc ttaatatctg
    28681 atggtgatat ttctccaaga cataggatat caacattatg actcatcaaa atatttgtta
    28741 taagagcaaa tgctacatcc aactcttcag gagtagaacg atctctttta accgcaggag
    28801 atatccccgt attccaccaa gcgattgaaa cagcctcaca cattcattca t
//
LOCUS       DADSYM010000053        28824 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2415
            SAMN08039579-rid11723173.denovo.053, whole genome shotgun sequence.
ACCESSION   DADSYM010000053 DADSYM010000000
VERSION     DADSYM010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039579
            Sequence Read Archive: SRR6362533
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 28824)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28824)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980162.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 03:39:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,484
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 4,347
            CDSs (with protein)               :: 4,347
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 43 of 85
            Pseudo Genes (incomplete)         :: 37 of 85
            Pseudo Genes (internal stop)      :: 19 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28824
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039579-rid11723173.denovo.053"
                     /strain="LGE2415"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8302 N 95.9628 W"
                     /collection_date="2015-08-24"
     gene            180..485
                     /locus_tag="J1Y83_001923"
     CDS             180..485
                     /locus_tag="J1Y83_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309170.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA7630073.1"
                     /translation="MKHKLSAILMAFMLTTPAAFAAPEAANGTEATTGTTGTTTTTTG
                     ATTTAATTGGVAAGAVGTATAVGVATAVGVATLAVVAANDSGDGGSHNTSTTTSTTR"
     gene            592..1236
                     /locus_tag="J1Y83_001924"
     CDS             592..1236
                     /locus_tag="J1Y83_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbF family lipoprotein"
                     /protein_id="HBA7630074.1"
                     /translation="MRPLILSIFALFLAGCTHSQQSMVDTFRASLFDNQDITVADQQI
                     QALPYSTMYLRLNEGQRIFVVLGYIEQEQSKWLSQDNAMLVTHNGRLLKTVKLNNNLL
                     EVTNSGQDPLRNALAIKDGSRWTRDILWSEDNHFRSATLSSTFSFAGLETLNIAGRDV
                     LCNVWQEEVTSTLPEKQWQNTFWVDSATGQVRQSRQMLGAGVIPVEMTFLKPAP"
     gene            1233..1979
                     /locus_tag="J1Y83_001925"
     CDS             1233..1979
                     /locus_tag="J1Y83_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule biosynthesis GfcC family protein"
                     /protein_id="HBA7630075.1"
                     /translation="MNKLQSYFIASVLYVMTPHAFAQGMVTIYLPGEQQTLSVGPVEN
                     VAQLVTQPQLRDRLWWPGALLTDSAAKAKALKDYQHVMAQLASWEAEADDDVAATIKS
                     VRQQLLNLNITGRLPVKLDPDFVRVDENSNPPLVGDYTLYTVQRPVTITLLGAVSGAG
                     QLPWLAGRSVTDYLQDHTRLAGADKNNVMVITPEGEAVVAPVALWNKRHVEPPPGSQL
                     WLGFSAHVLPEKYADLNDQIVSVLTQRVPD"
     gene            1979..4075
                     /locus_tag="J1Y83_001926"
     CDS             1979..4075
                     /locus_tag="J1Y83_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbH domain-containing protein"
                     /protein_id="HBA7630076.1"
                     /translation="MKKNSYLLSCLAIAVSSACHAEVLTYPDPLGSSQSDFGGTGLLQ
                     MPNARIAPEGEFSVNYRDNDQYRFYSTSVALFPWLEGTIRYTDVRTRKYSQWEDFSGD
                     QSYKDKSFDFKLRLWEEGYWLPQVAFGKRDIAGTGLFDGEYLVASKQAGPFDFTLGMA
                     WGYAGNAGNITNPFCRVSDKYCHRAESHDAGDISFSDIFRGPASIFGGIEYQTPWNPL
                     RLKLEYDGNNYQNDFAGKLPQATHFNVGAVYRAANWADLNLSYERGNTLMFGFTLRTN
                     FNDLRPALRDTPKPAYQPAPESEGLQYTTVANQLTALKYNAGFEAPEIQLRDKTLYMS
                     GQQYKYRDSREAVDRANRILVNNLPQGVEKISVTQKREHMAMVTTETDVASLRKQLAG
                     TAPGQSEPLQQQRVEAEDLSAFGRGYRIREDRFSYSFNPTLSQSLGGPEDFYMFQLGL
                     MSSTRYWFTDHLLLDGGIFTNIYNNYDKFKSSLLPADSTLPRVRTHIRDYVRNDVYLN
                     NLQANYFADLGNGFYGQVYGGYLETMYAGVGSELLYRPLDASWALGVDVNYVKQRDWD
                     NMMRFTDYSTPTGFVTAYWNPPTLNGVLMKLSVGQYLAKDKGATIDVAKRFDSGVAVG
                     IWAAISNVSKDDYGEGGFSKGFYISIPFDLMTIGPNRNRAVVSWTPLTRDGGQMLSRK
                     YQLYPMTAEREVPVGQ"
     gene            4121..5260
                     /locus_tag="J1Y83_001927"
     CDS             4121..5260
                     /locus_tag="J1Y83_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide export protein"
                     /protein_id="HBA7630077.1"
                     /translation="MKKNIFKFSVLTLAVLSLTACTLVPGQNLSTSNKDVIELPDNQY
                     DLDKMVNIYPVTPGLIDQLRAKPIMSQANPELEQQIANYEYRIGIGDVLMVTVWDHPE
                     LTTPAGQYRSASDTGNWVNADGAIFYPYIGRLKVAGKTLTQVRNEITARLDSVIESPQ
                     VDVSVAAFRSQKAYVTGEVSKSGQQPITNIPLTIMDAINAAGGLTADADWRNVVLTQN
                     GVKTKVNLYALMQRGDLRQNKLLHPGDILFIPRNDDLKVFVMGEVGKQSTLKMDRSGM
                     TLAEALGNAEGMNQDVADATGIFVIRATQNKQNGKIANIYQLNAKDASAMILGTEFQL
                     EPYDIVYVTTAPLARWNRVISLLVPTITGVHDLTETSRWIQTWPN"
     gene            5248..5694
                     /gene="etp"
                     /locus_tag="J1Y83_001928"
                     /gene_synonym="yccY"
     CDS             5248..5694
                     /gene="etp"
                     /locus_tag="J1Y83_001928"
                     /gene_synonym="yccY"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-tyrosine-phosphatase Etp"
                     /protein_id="HBA7630078.1"
                     /translation="MAQLKFNSILVVCTGNICRSPIGERLLRKRLPGVKVKSAGVHGL
                     VKHPADATAADVAANHGVSLEGHAGRKLTAEMARNYDLILAMESEHIAQVTAIAPEVR
                     GKTMLFGQWLEQKEIPDPYRKSQDAFEHVYGMLERASQEWAKRLSR"
     gene            5714..7894
                     /gene="etk"
                     /locus_tag="J1Y83_001929"
     CDS             5714..7894
                     /gene="etk"
                     /locus_tag="J1Y83_001929"
                     /EC_number="2.7.10.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein kinase"
                     /protein_id="HBA7630079.1"
                     /translation="MTTKNMNTPPGSTQENEIDLLRLVGELWDHRKFIISVTALFTLI
                     AVAYSLLSTPIYQADTLVQVEQKQGNAILSGLSDMIPNSSPESAPEIQLLQSRMILGK
                     TIAELNLRDIVEQKYFPIVGRGWARLTKEKPGELAISWMHIPQLNGQDQQLTLTVGEN
                     GHYTLEGEEFTVNGMVGQRLEKDGVALTIADIKAKAGTQFVLSQRTELEAINALQETF
                     TVSERSKESGMLELTMTGDDPQLITHILNSIANNYLQQNIARQAAQDSQSLEFLQRQL
                     PQVRSELDQAEEKLNAYRQQRDSVDLNLEAKAVLEQIVNVDNQLNELTFREAEISQLY
                     KKDHPTYRALLEKRQTLEQERKRLNKRVSAMPSTQQEVLRLSRDVEAGRAVYLQLLNR
                     QQELSISKSSAIGNVRIIDPAVTQPQPVKPKKALNVVLGFILGLFISVGAVLTRAMLR
                     RGVEAPEQLEEHGISVYATIPMSEWLDKRTRLRKKNLFSNQQRHRTKNIPFLAVDNPA
                     DSAVEAVRALRTSLHFAMMETENNILMITGATPDSGKTFVSSTLASVIAQSDQKVLFI
                     DADLRRGYSHNLFNVSNEHGLSEYLAGKDELNKVIQHFGKGGFDVITRGQVPPNPSEL
                     LMRDRMRQLLEWANDHYDLVIVDTPPMLAVSDAAVVGRYVGTSLLVARFSLNTAKEVS
                     LSMQRLEQAGVNIKGAILNGVIKRASTAYSYGYNYYGYSYSEKE"
     gene            complement(8009..9307)
                     /locus_tag="J1Y83_001930"
     CDS             complement(8009..9307)
                     /locus_tag="J1Y83_001930"
                     /EC_number="3.1.3.2"
                     /EC_number="3.1.3.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AppA family phytase/histidine-type acid
                     phosphatase"
                     /protein_id="HBA7630080.1"
                     /translation="MKAILIPFLSLLIPLTPKSAFAQSEPELKLESVVIVSRHGVRAP
                     TKATQLMQDVTPDAWPTWPVKLGWLTPRGGELIAYLGHYQRQRLVADGLLAKKGCPQP
                     GQVAVIADVDERTRKTGEAFAAGLAPDCAITVHTQADTSSPDPLFNPLKTGVCQLDNA
                     NVTDAILSRAGGAIADFTGHRQTAFRELEQVLNFPQSNLCLNREKQDESCSLTQALPS
                     ELKVSADNVSLTGAVSLASMLTEIFLLQQAQGMPEPGWGRITDSHQWNTLLSLHNAQF
                     YLLQRTPEVARSRATPLLDLIMTALTPHPPQKQVYGVTLPTSVLFIAGHDTNLANLGG
                     ALELNWTLPGQPDNTPPGGELVFERWRRLSDNSHWIQVSLVFQTLQQMRDKTPLSLNT
                     PPGEVKLTLAGCEERNAQGMCSLAGFTQIVNEARIPACSL"
     gene            complement(9387..9479)
                     /gene="cbdX"
                     /locus_tag="J1Y83_001931"
     CDS             complement(9387..9479)
                     /gene="cbdX"
                     /locus_tag="J1Y83_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-II oxidase subunit CbdX"
                     /protein_id="HBA7630081.1"
                     /translation="MWYLLWFVGILLMCSLSTLVLVWLDPRLKS"
     gene            complement(9492..10628)
                     /gene="appB"
                     /locus_tag="J1Y83_001932"
     CDS             complement(9492..10628)
                     /gene="appB"
                     /locus_tag="J1Y83_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HBA7630082.1"
                     /translation="MFDYETLRFIWWLLIGVILVVFMISDGFDMGIGCLLPLVARNDD
                     ERRIVINSVGAHWEGNQVWLILAGGALFAAWPRVYAAAFSGFYVAMILVLCSLFFRPL
                     AFDYRGKIADARWRKMWDAGLVIGSLVPPVVFGIAFGNLLLGVPFAFTPQLRVEYLGS
                     FWQLLTPFPLLCGLLSLGMVILQGGVWLQLKTVGVIHLRSQLATKRAALLVMLCFLLA
                     GYWLWVGIDGFVLLAQDANGPSNPLMKLVAVLPGAWMNNFVESPVLWIFPLLGFFCPL
                     LTVMAIYRGRPGWGFLMASLMQFGVIFTAGITLFPFVMPSSVSPISSLTLWDSTSSQL
                     TLSIMLVIVLIFLPIVLLYTLWSYYKMWGRMTTETLRRNENELY"
     gene            complement(10640..12184)
                     /gene="appC"
                     /locus_tag="J1Y83_001933"
     CDS             complement(10640..12184)
                     /gene="appC"
                     /locus_tag="J1Y83_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-II oxidase subunit 1"
                     /protein_id="HBA7630083.1"
                     /translation="MWDVIDLSRWQFALTALYHFLFVPLTLGLIFLLAIMETIYVVTG
                     KTIYRDMTRFWGKLFGINFALGVATGLTMEFQFGTNWSFYSNYVGDIFGAPLAMEALM
                     AFFLESTFVGLFFFGWQRLNKYQHLLVTWLVAFGSNLSALWILNANGWMQYPTGAHFD
                     IDTLRMEMTSFSELVFNPVSQVKFVHTVMAGYVTGAMFIMAISAWYLLRGRERDVALR
                     SFAIGSVFGTLAIIGTLQLGDSSAYEVAQVQPVKLAAMEGEWQTEPAPAPFHVVAWPE
                     QDQERNAFAIKIPALLGILATHSLDKPVPGLKNLMAETYPRLQRGRMAWLLMQEISQG
                     NREPHVLQAFRELEGDLGYGMLLSRYAPDMNHVTAAQYQAAMRGAIPQVAPVFWSFRI
                     MVGCGSLLLLVMLIALVQTLRGKIDQHRWVLKMALWSLPLPWIAIEAGWFMTEFGRQP
                     WAIQDILPTYSAHSALTTGQLAFSLIMIVGLYTLFLIAEVYLMQKYARLGPSAMQSEQ
                     PTQQQG"
     gene            complement(12318..13175)
                     /locus_tag="J1Y83_001934"
     CDS             complement(12318..13175)
                     /locus_tag="J1Y83_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase expression/formation protein"
                     /protein_id="HBA7630084.1"
                     /translation="MSETFFHLLGPGTQPNDDSFSMNPLPITCRVNGEPSMAALEQCA
                     HSPQVIALLNELQHQLSERQPPLGEVLAVDLLNLNADDRHFINTLLGEGEVSVRIQQA
                     DDSESEIQEAIFCGLWRVRRRHGEQLLEDKLEAGCAPLALWQAATQNVLPTDSLLPPP
                     IDGLMNGLPLAHELLAHVRNPDAQPHSINLTQLPISEADRLFLSRLCGPGNIQIRTIG
                     YGESYINATGLRHVWHLRCTDTLKGPLLESYEICPIPEVVLAAPEDLVDSAQRLSEVC
                     QWLAEGAPT"
     gene            complement(13172..13570)
                     /gene="hyaE"
                     /locus_tag="J1Y83_001935"
     CDS             complement(13172..13570)
                     /gene="hyaE"
                     /locus_tag="J1Y83_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase-1 operon protein HyaE"
                     /protein_id="HBA7630085.1"
                     /translation="MSNDTPFDALWQRMLARGWTPVSECRLDDWLTQAPDGVVLLSSD
                     PKRTPEVSDNPVMIGELLREFPDYTWQVAIADLEQSEAIGDRFGVFRFPATLVFTGGN
                     YRGVLNGIHPWAELINLMRGLVEPQQERAS"
     gene            complement(13567..14154)
                     /locus_tag="J1Y83_001936"
     CDS             complement(13567..14154)
                     /locus_tag="J1Y83_001936"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 1 maturation protease"
                     /protein_id="HBA7630086.1"
                     /translation="MSEQRVVVMGLGNLLWADEGFGVRVAERLYAHYHWPEDVEIVDG
                     GTQGLNLLGYVESASHLLILDAIDYGLEPGTLRTYAGERIPAYLSAKKMSLHQNSFSE
                     VLALADIRGHLPAHIALVGLQPAMLDDYGGSLSELAREQLPAAEQAALVQLAAWGIVP
                     QPTEELRCLNYDCLSMENYEGVRLRQYKIRLEEQG"
     gene            complement(14151..14858)
                     /gene="hyaC"
                     /locus_tag="J1Y83_001937"
     CDS             complement(14151..14858)
                     /gene="hyaC"
                     /locus_tag="J1Y83_001937"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase b-type cytochrome subunit"
                     /protein_id="HBA7630087.1"
                     /translation="MQQKSDNVVSHYVFEAPVRIWHWLTVLCMAVLMVTGYFIGKPLP
                     SVSGEATYLFYMGYIRLIHFSAGMIFTVVLLMRIYWAFVGNRYSRELFIVPVWRKSWW
                     QGVWYEIRWYLFLAKRPSADIGHNPIAQAAMFGYFLMSVFMIITGFALYSEHSQYAIF
                     APFRYVVEFFYWTGGNSMDIHSWHRLGMWLIGAFVIGHVYMALREDIMSDDTVISTMV
                     NGYRSHKFGKISNKERS"
     gene            complement(14877..16670)
                     /gene="hyaB"
                     /locus_tag="J1Y83_001938"
     CDS             complement(14877..16670)
                     /gene="hyaB"
                     /locus_tag="J1Y83_001938"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase large subunit"
                     /protein_id="HBA7630088.1"
                     /translation="MSTQYETQGYTINNAGRRLVVDPITRIEGHMRCEVNINDQNVIT
                     NAVSCGTMFRGLEIILQGRDPRDAWAFVERICGVCTGVHALASVYAIEDAIGIKVPDN
                     ANIIRNIMLATLWCHDHLVHFYQLAGMDWIDVLDALKADPRKTSELAQSLSSWPKSSP
                     GYFFDVQNRLKKFVEGGQLGIFRNGYWGHPQYKLPPEANLMGFAHYLEALDFQREIVK
                     IHAVFGGKNPHPNWIVGGMPCAINIDESGAVGAVNMERLNLVQSIITRTADFINNVMI
                     PDALAIGQFNKPWSEIGTGLSDKCVLSYGAFPDIANDFSEKSLLMPGGAVINGDFNNV
                     LPVDLVDPQQVQEFVDHAWYRYPNDQVGRHPFDGITDPWYNPGDVKGSDTNIQQLNEQ
                     ERYSWIKAPRWRGNAMEVGPLARTLIAYHKGDAATVESVDRMMSALKLPLSGIQSTLG
                     RILCRAHEAQWAAGKLQYFFDKLMTNLKNGNLATASTEKWEPATWPTECRGVGFTEAP
                     RGALGHWAAIRDGKIDLYQCVVPTTWNASPRDPKGQIGAYEAALMNTKMAIPEQPLEI
                     LRTLHSFDPCLACSTHVLGDDGSELISVQVR"
     gene            complement(16667..17785)
                     /gene="hyaA"
                     /locus_tag="J1Y83_001939"
     CDS             complement(16667..17785)
                     /gene="hyaA"
                     /locus_tag="J1Y83_001939"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 1 small subunit"
                     /protein_id="HBA7630089.1"
                     /translation="MNNEETFYQAMRRQGVTRRSFLKYCSLAATSLGLGAGMAPKIAW
                     ALENKPRIPVVWIHGLECTCCTESFIRSAHPLAKDVILSLISLDYDDTLMAAAGTQAE
                     EVFEDIITQYNGKYILAVEGNPPLGEQGMFCISSGRPFIEKLKRAAAGASAIIAWGTC
                     ASWGCVQAARPNPTQATPIDKVITDKPIIKVPGCPPIPDVMSAIITYMVTFDRLPDVD
                     RMGRPLMFYGQRIHDKCYRRAHFDAGEFVQSWDDDAARKGYCLYKMGCKGPTTYNACS
                     STRWNDGVSFPIQSGHGCLGCAENGFWDRGSFYSRVVDIPQMGTHSTADTVGLTALGV
                     VAAAVGVHAVASAVDQRRRHNQQPTETEHQPGNEDKQA"
     gene            18212..18299
                     /locus_tag="J1Y83_001940"
     tRNA            18212..18299
                     /locus_tag="J1Y83_001940"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:18246..18248,aa:Ser,seq:tga)
     gene            18508..19167
                     /gene="yccA"
                     /locus_tag="J1Y83_001941"
     CDS             18508..19167
                     /gene="yccA"
                     /locus_tag="J1Y83_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000375124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease modulator YccA"
                     /protein_id="HBA7630090.1"
                     /translation="MDRIVSSSHDRTSLLSTHKVLRNTYFLLSLTLAFSAITATASTV
                     LMLPSPGLILTLVGMYGLMFLTYKTANKPTGIISAFAFTGFLGYILGPILNTYLSAGM
                     GDVIAMALGGTALVFFCCSAYVLTTRKDMSFLGGMLMAGIVVVLIGMVANIFLQLPAL
                     HLAISAVFILISSGAILFETSNIIHGGETNYIRATVSLYVSLYNIFVSLLSILGFASR
                     D"
     gene            19259..19588
                     /gene="tusE"
                     /locus_tag="J1Y83_001942"
     CDS             19259..19588
                     /gene="tusE"
                     /locus_tag="J1Y83_001942"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusE"
                     /protein_id="HBA7630091.1"
                     /translation="MLIFEGKEIETDTEGYLKESSQWSEPLAVVIAENEGIALSPEHW
                     EVVRFVRDFYLEFNTSPAIRMLVKAMANKFGEEKGNSRYLYRLFPKGPAKQATKIAGL
                     PKPVKCI"
     gene            complement(19585..19863)
                     /gene="yccX"
                     /locus_tag="J1Y83_001943"
     CDS             complement(19585..19863)
                     /gene="yccX"
                     /locus_tag="J1Y83_001943"
                     /EC_number="3.6.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HBA7630092.1"
                     /translation="MSKVCIIAWVYGRVQGVGFRYTTQYEAKKLGLTGYAKNLDDGSV
                     EVVACGEEGQVEKLMQWLKSGGPRSARVERVLSEPHHPSGELTDFRIR"
     gene            19958..21148
                     /gene="rlmI"
                     /locus_tag="J1Y83_001944"
     CDS             19958..21148
                     /gene="rlmI"
                     /locus_tag="J1Y83_001944"
                     /EC_number="2.1.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415487.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (cytosine(1962)-C(5))-methyltransferase
                     RlmI"
                     /protein_id="HBA7630093.1"
                     /translation="MSVRLVLAKGREKSLLRRHPWVFSGAVARMEGKASLGETIDIVD
                     HQGKWLARGAYSPASQIRARVWTFDPSESIDIAFFTRRLQQAQKWRDWLAQKDGLDSY
                     RLIAGESDGLPGITIDRFGNFLVLQLLSAGAEYQRAALISALQTLYPECAIYDRSDVA
                     VRKKEGMELTQGPITGELPPALLPIEEHGMKLLVDIQHGHKTGYYLDQRDSRLATRRY
                     VENKRVLNCFSYTGGFAVSALMGGCSQVVSVDTSQEALDIARQNVELNKLDLSKAEFV
                     RDDVFKLLRTYRDRGEKFDVIVMDPPKFVENKSQLMGACRGYKDINMLAIQLLNEGGV
                     LLTFSCSGLMTSDLFQKIIADAAIDAGRDVQFIEQFRQAADHPVIATYPEGLYLKGFA
                     CRVM"
     gene            21206..21523
                     /gene="hspQ"
                     /locus_tag="J1Y83_001945"
     CDS             21206..21523
                     /gene="hspQ"
                     /locus_tag="J1Y83_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock protein HspQ"
                     /protein_id="HBA7630094.1"
                     /translation="MIASKFGIGQQVRHSLLGYLGVVVDIDPVYSLSEPSPDELAVND
                     ELRAAPWYHVVMEDDNGLPVHTYLAEAQLSSELQDEHPEQPSMDELAQTIRKQLQAPR
                     LRN"
     gene            complement(21568..21981)
                     /locus_tag="J1Y83_001946"
     CDS             complement(21568..21981)
                     /locus_tag="J1Y83_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HBA7630095.1"
                     /translation="MKETDIAGILTSTHTIALVGASDKPDRPSYRVMKYLLDQGYHVI
                     PVSPKVAGKTLLGQQGYGTLADVPEKVDMVDVFRNSEAAWGVAQEAIAIGAKTLWMQL
                     GVINEQAAVLARDAGLNVVMDRCPAIEIPRLGLAK"
     gene            22154..22816
                     /locus_tag="J1Y83_001947"
     CDS             22154..22816
                     /locus_tag="J1Y83_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2057 family protein"
                     /protein_id="HBA7630096.1"
                     /translation="MKTGIVTTLIALCLPVSVFATTLRLSTDVDLLVLDGKKVSSSLL
                     RGADSIELDNGPHQLVFRVEKTIHLSNSEERLYISPPLVVSFNTQLINQVNFRLPRLE
                     NEREANHFDAAPRLELLDGDATPIPVKLDILAITSTAKTIDYEVEVERYNKSAKRASL
                     PQFATMMADDSTLLSGVSELDAIPPQSQVLTEQRLKYWFKLADPQTRNTFLQWAEKQP
                     SS"
     gene            22912..23370
                     /gene="mgsA"
                     /locus_tag="J1Y83_001948"
     CDS             22912..23370
                     /gene="mgsA"
                     /locus_tag="J1Y83_001948"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415483.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HBA7630097.1"
                     /translation="MELTTRTLPARKHIALVAHDHCKQMLMSWVERHQPLLEQHVLYA
                     TGTTGNLISRATGMNVNAMLSGPMGGDQQVGALISEGKIDVLIFFWDPLNAVPHDPDV
                     KALLRLATVWNIPVATNVATADFIIQSPHFNDAVDILIPDYQRYLADRLK"
     gene            complement(23402..25456)
                     /gene="helD"
                     /locus_tag="J1Y83_001949"
     CDS             complement(23402..25456)
                     /gene="helD"
                     /locus_tag="J1Y83_001949"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase IV"
                     /protein_id="HBA7630098.1"
                     /translation="MELKATTLGKRLAQHPYDRAVILNAGIKVSGDRHEYLIPFNQLL
                     AIHCKRGLVWGELEFVLPDEKVVRLHGTEWGETQRFYHHLDAHWRRWSSEMSEIASGV
                     LRQQLDLIATRTGENKWLTREQTSGVQQQIRQALSALPLPVNRLEEFDNCREAWRKCQ
                     AWLKDIEGARLQHNQAYTEAMLTEYADFFRQVESSPLNPAQARAVVNGEHSLLVLAGA
                     GSGKTSVLVARAGWLLARGEASPEQILLLAFGRKAAEEMDERIRERLHTEDITARTFH
                     ALALHIIQQGSKKVPIVSKLENDTAARHELFIAEWCKQCSEKKAQAKGWRQWLTEEMQ
                     WSVPEGNFWDDEKLQRRLASRLDRWVSLMRMHGGAQAEMIASAPEEIRDLFSKRIKLM
                     APLLKAWKGALKAENAVDFSGLIHQAIVILEKGRFISPWKHILVDEFQDISPQRAALL
                     AALRKQNSQTTLFAVGDDWQAIYRFSGAQMSLTTAFHENFGEGDRCDLDTTYRFNSRI
                     GEVANRFIQQNPGQLKKPLNSLTNGDKKAVTLLDESHLDALLDKLSGYAKPEERILIL
                     ARYHHMRPASLEKAATRWPKLQIDFMTIHASKGQQADYVIIVGLQEGSDGFPAAARES
                     IMEEALLPPVEDFPDAEERRLMYVALTRARHRVWALFNKENPSPFVEILKNLDVPVAR
                     KP"
     gene            25579..26025
                     /locus_tag="J1Y83_001950"
     CDS             25579..26025
                     /locus_tag="J1Y83_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HBA7630099.1"
                     /translation="MRTVLNILNFVLGGFATTLGWLLATLVSIVLIFTLPLTRSCWEI
                     TKLSLVPYGNEAIHVDELNPAGKNVLLNTGGTVLNIFWLIFFGWWLCLMHIATGIAQC
                     ISIIGIPVGIANFKIAAIALWPVGRRVVSVETAQAAREANARRRFE"
     gene            26035..28197
                     /gene="yccS"
                     /locus_tag="J1Y83_001951"
     CDS             26035..28197
                     /gene="yccS"
                     /locus_tag="J1Y83_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309071.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01666 family membrane protein"
                     /protein_id="HBA7630100.1"
                     /translation="MAFMLSPLLKRYTWNSAWLYYARIFIALCGTTAFPWWLGDVKLT
                     IPLTLGMVAAALTDLDDRLAGRLRNLIITLFCFFIASTSVELLFPWPWLFAIGLTLST
                     SGFILLGGLGQRYATIAFGALLIAIYTMLGTSLYEHWYQQPMYLLAGAVWYNVLTLIG
                     HLLFPVRPLQDNLARCYEQLARYLELKSRMFDPDIEDESQAPLYDLALANGQLMATLN
                     QTKLSLLTRLRGDRGQRGTRRTLHYYFVAQDIHERASSSHIQYQTLREHFRHSDVLFR
                     FQRLMSMQGQACQQLSRCILLRQPYQHDPHFERAFTHIDAALERMRDNGAPADLLKTL
                     GFLLNNLRAIDAQLATIESEQAQALPHNNDENELADDSPHGLSDIWLRLSRHFTPESA
                     LFRHAVRMSLVLCFGYAIIQITGMHHGYWILLTSLFVCQPNYNATRHRLKLRIIGTLV
                     GIAIGIPVLWFVPSLEGQLVLLVITGVLFFAFRNVQYAHATMFITLLVLLCFNLLGEG
                     FEVALPRVIDTLIGCAIAWAAVSYIWPDWKFRNLPRMLERATEANCRYLDAILEQYHQ
                     GRDNRLAYRIARRDAHNRDAELASVVSNMSSEPNVTPQIREAAFRLLCLNHTFTSYIS
                     ALGAHREQLTNPEILAFLDDAVCYVDDALHHQPADEERVNQALAGLKQRMQQLEPRAD
                     SKEPLVVQQVGLLIALLPEIGRLQRQITQVPQETPVSA"
     gene            complement(28160..28789)
                     /gene="sxy"
                     /locus_tag="J1Y83_001952"
     CDS             complement(28160..28789)
                     /gene="sxy"
                     /locus_tag="J1Y83_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRP-S regulon transcriptional coactivator Sxy"
                     /protein_id="HBA7630101.1"
                     /translation="MKSLSYKRIYKSQEYLATLGTIEYRSLFGSYSLTVDDTVFAMVS
                     DGELYLRACEQSAPYCVKHPPVWLTYKKCGRSVTLNYYRVDESLWRNQLKLVRLSKYS
                     LDAALKEKSTRNIRERLKDLPNMSFHLEAILGEVGIKDVRALRILGAKMCWLRLRQQN
                     SLVTEKILFMLEGAIIGIHEAALPVARRQELAEWADSLTPKQEFPAELE"
BASE COUNT         6915 a         7633 c         7337 g         6939 t
ORIGIN      
        1 cgtatttcca gagggaaatc gttaacaatc ttatatttcc ctctgcgcgc ctttcctacc
       61 cgcagtatat agtgcaagcg gtatgaggaa agggagattt cacaaggatg ttatactcaa
      121 agtagataat cctgatctca agcgtacgta ttgtccatgc gatgaataaa ggaaaagtta
      181 tgaaacacaa gctttctgca atccttatgg ccttcatgtt gacgacgcca gctgcatttg
      241 ctgcccctga agcagcaaac gggaccgagg caacgacggg tacaactggc accacaacga
      301 ccactaccgg tgcaaccacg actgcagcta ccactggtgg cgtggctgct ggcgctgtcg
      361 gtacggcgac ggctgtcggt gtggcaacgg ctgttggtgt ggcgacgttg gcagttgttg
      421 cagcaaatga cagtggcgat ggcggttctc ataacacctc aaccaccacg tccactacgc
      481 gctaatacca ctttaacgcg tcgaaattaa agttaaatga tacccggttg cgatattcgt
      541 accgggcttt agagatttgc atttttttaa ttaacctttc aaggattaaa ggtgcgccct
      601 cttattttat cgattttcgc actatttctt gcgggatgta cgcacagcca gcaaagtatg
      661 gtcgatacat ttcgcgccag cctattcgat aatcaggata tcaccgtagc ggatcagcag
      721 atccaggcgt tgccttattc cacgatgtat ttacgcctta atgaagggca acgaatcttt
      781 gtggtactgg gatatataga acaagaacaa agcaaatggt tatcccagga taacgccatg
      841 ctggttaccc acaatggacg tcttttaaaa accgtcaaac ttaataataa tctgctggaa
      901 gtgacaaatt ccgggcagga ccctctgcgt aacgcgctgg caataaaaga cggcagccgc
      961 tggacgcgcg atattctctg gagcgaagac aaccattttc gctctgcgac cctgagttcc
     1021 actttttcct ttgctggatt agagacgctg aatattgcgg gtcgcgatgt gctgtgtaat
     1081 gtctggcagg aagaggtgac ttccacgctg ccagaaaaac agtggcaaaa cacattctgg
     1141 gtcgattcgg ctactggcca ggttcgtcaa agtcggcaaa tgttaggcgc aggggttatt
     1201 cccgtagaaa tgacgtttct taaacccgca ccatgaataa attacagtcg tatttcattg
     1261 ccagcgtcct ttacgtaatg acaccccatg cctttgcgca aggaatggtg actatttatc
     1321 tgcctggcga acaacagaca ctttccgttg gccccgtaga gaatgttgcc cagttggtga
     1381 cacaaccgca actgcgcgat cgcctctggt ggcctggtgc cttgctgacc gactccgcag
     1441 cgaaagctaa agcgcttaaa gactaccaac atgtcatggc acagcttgct tcctgggaag
     1501 cagaagctga cgacgatgtg gcggcaacga ttaaatccgt gcgccagcag ttactcaatc
     1561 tcaatatcac tggtcgctta ccggtcaaac tggaccctga ttttgtgcga gtggatgaga
     1621 acagcaatcc cccgctggtc ggggactata cgctctatac cgtacagcgt ccggtgacta
     1681 ttactctgct gggtgcggtt tctggggctg ggcaattgcc ctggctggca ggccgtagcg
     1741 ttaccgatta tctgcaagat cacacccgtc ttgcaggcgc ggacaagaat aacgttatgg
     1801 tgattacgcc tgagggtgag gctgtcgtcg cccctgttgc cctgtggaat aaaagacatg
     1861 tggaaccacc gcctggcagc cagctttggt taggcttttc tgcgcatgta ctgcccgaaa
     1921 aatatgctga tttaaatgac cagattgtgt ctgttttaac gcaacgtgtt cctgactgat
     1981 gaagaagaac tcttatcttt taagctgcct ggccattgcc gtctccagtg cctgtcatgc
     2041 tgaagtatta acctacccgg atccgctggg ttcgtcgcaa tcagactttg gcggcacagg
     2101 attgttgcag atgccaaatg cgcgcatcgc accggaaggt gaattcagcg tcaactaccg
     2161 ggataacgat cagtaccgtt tctactccac gtccgtggcg ctgttcccat ggctggaagg
     2221 caccattcgt tatacggatg tgcgcacacg caaatatagc cagtgggaag atttcagcgg
     2281 cgatcagtca tacaaagaca aatcattcga ttttaaactt cgcctgtggg aagaaggtta
     2341 ctggctaccg caagtggcgt ttggtaaacg tgatattgct ggtacgggtc tgttcgacgg
     2401 tgaatatctg gtcgccagca aacaagccgg accgttcgat ttcaccctcg ggatggcctg
     2461 gggctacgcc ggtaatgcgg gcaatattac caacccgttt tgtcgggtaa gcgataaata
     2521 ttgccatcgc gcagagtctc acgacgcggg agatatcagc tttagcgata tctttcgtgg
     2581 tccagcctct atttttggcg gtattgagta tcaaacgccg tggaatcctc tgcgtctgaa
     2641 actcgaatac gacggcaaca attaccagaa tgattttgcg ggcaaattgc cgcaggcaac
     2701 tcatttcaat gttggtgctg tgtatcgtgc tgccaactgg gcagatctta accttagcta
     2761 cgagcgtggt aacacgttga tgtttggctt cacgttacga accaatttca acgatctgcg
     2821 ccctgccctg cgcgatacgc caaaaccggc atatcaacct gcgccggaat ctgaaggatt
     2881 gcagtacacc acggtagcaa accaacttac cgcactgaag tacaacgcag gttttgaagc
     2941 accggaaatt cagctacgcg ataagacgct gtatatgtct ggtcagcaat acaaataccg
     3001 tgactctcgc gaagcggtcg atcgtgccaa ccggattctg gtgaataacc tgccgcaagg
     3061 cgttgagaag attagcgtga cgcaaaagcg cgagcatatg gcgatggtga ctaccgaaac
     3121 cgacgtagcc agcctgcgca aacagttggc cggtacagcg cctggtcaat cagagccact
     3181 gcaacaacaa cgtgttgaag ctgaagatct ttctgccttt ggtcggggct atcgtattcg
     3241 tgaagatcgc tttagctact ctttcaaccc aacactttca cagtcgctgg gcggcccgga
     3301 agatttctat atgttccagt tggggctgat gtccagcacc cgctactggt ttaccgacca
     3361 cctgctactt gatggcggta ttttcaccaa tatttacaac aactacgaca agtttaagtc
     3421 ttcgctgtta cccgcggact ctaccctgcc ccgcgtgcgc acgcatatcc gtgattacgt
     3481 tcgcaatgac gtttatctca acaacttgca ggcgaactac tttgccgact taggcaatgg
     3541 tttctatggc caggtgtatg gcggttatct ggaaacgatg tatgccggtg tcggttccga
     3601 gctgctttat cgcccgctag atgccagctg ggcgctgggt gtggacgtta actacgtgaa
     3661 gcagcgtgac tgggacaaca tgatgcgctt caccgattat tccacgccaa caggtttcgt
     3721 gacggcttac tggaacccgc cgacgctcaa tggcgtactg atgaaactca gcgtggggca
     3781 atatctggca aaagataaag gcgcaacgat tgacgttgcc aaacgctttg acagcggtgt
     3841 ggcagtagga atatgggcgg caatcagtaa cgtatcaaaa gatgactacg gcgaaggcgg
     3901 ctttagtaaa gggttttata tctcgattcc gttcgacttg atgaccattg ggcctaaccg
     3961 caaccgcgcg gtggtttcgt ggacaccatt gacgcgtgat ggtggacaaa tgctgtcacg
     4021 caaataccag ctctatccaa tgacggcaga gcgagaagta ccggttggac aataacctga
     4081 agtaattcat aacattacaa tgactgataa atataaacca atgaaaaaga acatttttaa
     4141 gttttcggta ctgacattgg ctgttctgtc tttgacagca tgtacccttg ttcctggtca
     4201 gaatttatcg accagtaaca aggatgtcat tgaactacct gacaatcagt atgacctgga
     4261 caaaatggtc aatatctatc ccgtcacgcc aggattgatt gatcagctgc gcgccaaacc
     4321 gattatgtcg caggctaatc cggagctgga gcagcaaatc gcgaactatg aatatcgcat
     4381 cggcattggt gatgtgttga tggttacggt ctgggatcac ccggaactaa ccacccccgc
     4441 agggcaatac cgtagcgcca gtgataccgg caactgggtt aacgccgatg gtgcgatttt
     4501 ctacccctat attggtcgcc tgaaagtggc cgggaaaaca cttacccagg tacgtaacga
     4561 gattacagcg cgtctggatt cagtcattga aagcccgcag gtcgatgtca gcgtcgcggc
     4621 gttccgatcg caaaaagcgt acgtcaccgg cgaagtcagc aaatcgggtc agcagcctat
     4681 taccaatatt cctctgacca tcatggatgc gattaacgcc gcgggaggtc tgaccgcaga
     4741 tgcagactgg cgcaacgtgg tgctcactca gaatggcgta aagaccaaag taaacctcta
     4801 tgcgctgatg cagcgtggcg acttgcgcca gaacaaactg ctgcacccgg gcgatatttt
     4861 atttattccg cgcaacgatg atctgaaagt cttcgtgatg ggtgaagtgg gtaaacagag
     4921 cacgctgaaa atggaccgca gcggtatgac cctggcggaa gcgctgggta acgctgaggg
     4981 tatgaatcag gatgtcgccg acgcgacggg gatttttgtt attcgtgcaa cgcaaaacaa
     5041 gcagaatggc aaaatcgcca atatttatca gctgaatgcg aaggacgcat cggcaatgat
     5101 actcggaacg gaattccagc ttgaacctta cgacattgtt tatgtcacta ctgcgccgct
     5161 ggctcgttgg aaccgtgtga tttctctgct ggtaccgacg attaccgggg tgcatgacct
     5221 gacagagact tcacggtgga ttcaaacatg gcccaactaa aatttaactc aatcctggtg
     5281 gtttgtaccg gcaacatttg ccgttcgccc attggcgaac gcttgctgcg taaacgtctg
     5341 ccaggcgtaa aagtcaaatc agcaggtgtt catggtctgg taaaacaccc tgcagatgcg
     5401 acagcggcag atgttgctgc taatcatggc gtatctcttg aaggacatgc cggacgtaag
     5461 ctcactgctg aaatggcacg aaattacgat ctgattctgg caatggagtc agagcatatt
     5521 gctcaggtta ccgcaatcgc tcccgaggtt cgcgggaaga caatgctgtt tgggcaatgg
     5581 ctggaacaga aagagatccc ggatccctat cgtaaaagtc aggacgcatt tgaacatgtc
     5641 tacggtatgt tggagcgcgc cagtcaggaa tgggcgaagc gcctcagccg gtaatttgag
     5701 tttataaaat acgatgacaa ctaaaaatat gaatacgcca ccaggcagca ctcaggaaaa
     5761 tgagatcgat ctgcttcgtc tggtcggcga gttatgggat caccgtaagt ttattatcag
     5821 cgtgaccgcg ttattcacgc tgatcgctgt cgcttactcg ctgttaagca cgccaattta
     5881 tcaggcagat actctggtcc aggttgagca gaaacagggc aacgccattc tcagcggcct
     5941 gagcgatatg atccctaact catctcccga gtctgcaccg gagatccaac tgctgcaatc
     6001 gcgcatgatt ctcggtaaaa ccattgctga actgaatctg cgcgacatcg ttgagcagaa
     6061 gtattttccg attgtgggtc gcggctgggc gagattaacc aaagagaaac caggtgagct
     6121 ggcgatcagc tggatgcata taccacaact gaatggtcag gatcagcaac tgacgctgac
     6181 ggttggggaa aacggccact atacgctgga aggtgaagag ttcaccgtca atggtatggt
     6241 cggccagcgt ctggaaaaag atggcgttgc gctgactatc gcggacatta aggccaaagc
     6301 aggaacacag tttgtcctga gccagcgcac cgaactggaa gcgattaacg ccctgcagga
     6361 aacctttacc gttagcgagc gcagtaaaga gagcgggatg ctggaactca ccatgaccgg
     6421 tgacgatccg caactgatta ctcatatact gaacagcatc gctaacaact atttgcagca
     6481 gaatatcgcg cgtcaggcgg cacaggattc gcaaagtctt gagttcctgc agcgccagtt
     6541 accgcaggta cgcagtgagt tggatcaggc tgaggaaaaa ctgaatgcct atcgtcaaca
     6601 acgtgattcg gtagacttga atctggaagc caaagccgtt cttgagcaga ttgttaacgt
     6661 tgataatcaa ctcaatgagc tgaccttccg cgaggcagag atctcccagc tgtataagaa
     6721 agatcaccca acttatcgtg cgctgctgga aaaacgccag acgctggagc aagaacgcaa
     6781 acgcctgaat aagcgggtat cggcaatgcc ttccactcaa caggaagtgc tacgtttaag
     6841 ccgtgacgta gaagcgggcc gtgcggtata tctgcaatta cttaaccgcc agcaggagtt
     6901 gagtatttcg aaatccagtg ccattggtaa cgtgcggatt atcgacccgg cagtcactca
     6961 gccgcagcca gtgaaaccga aaaaagcgtt gaatgtggtg cttggtttta ttcttggcct
     7021 gtttatttcg gtgggtgccg tgctgacgcg tgcgatgttg cgtcgtggtg tagaagcacc
     7081 ggaacaactg gaagagcacg gcatcagcgt ttatgccact atcccgatgt ccgagtggct
     7141 ggataaacgc acccgtctgc gtaagaaaaa tttattttct aatcagcagc gccatcgtac
     7201 taaaaatatc cccttcctgg cggtggataa cccggcggat tctgctgtag aagccgtacg
     7261 tgcactacga accagtctgc atttcgctat gatggagacg gagaataaca ttctgatgat
     7321 caccggtgcg acgccagaca gtggtaaaac gttcgtcagt tcaactctgg cgtcggtgat
     7381 cgcccagtcc gatcaaaaag tgttatttat tgacgccgac ttacgccgtg gttattcgca
     7441 taacctgttc aacgtgagta atgaacatgg tttgtctgaa tatctggcag gtaaagatga
     7501 gctcaacaaa gtgatccagc attttggcaa aggaggcttt gatgtgatta ctcgcggtca
     7561 ggtgccacct aacccgtctg aactgctgat gcgcgatcgg atgcgtcaat tactggaatg
     7621 ggcgaacgac cattacgatc tggtgattgt cgatacgccg ccgatgctgg cggtgagcga
     7681 tgctgcggtc gtgggtcgct atgtcggcac cagcctgctg gttgcgcgtt ttagcctgaa
     7741 caccgccaaa gaggtgagct tgtcaatgca acgtctggaa caggcaggcg tcaatattaa
     7801 aggcgctatc ctcaatggtg tgattaaacg cgccagcacc gcttacagtt acggctataa
     7861 ctattacggt tatagttact ccgagaaaga gtaaaactga aagtggccgg atgcggcgtg
     7921 aacgcctttt ccggcctacg catcagacag ttcttcgttt gtcatcaacc tcagagcatt
     7981 caggtaactg aatgctcttt tttatgcatt acaaactgca cgctggtatg cgtgcttcat
     8041 tcacgatttg cgtaaaacct gccaacgaac acatgccctg cgcatttcgc tcttcacatc
     8101 ctgccagggt cagtttcacc tctccgggcg gcgtatttaa tgacagcggc gttttatcac
     8161 gcatctgctg taaagtctgg aagaccagcg aaacctgaat ccaatggctg ttatcgctta
     8221 gcctacgcca gcgttcaaac accagttcac cacctggcgg tgtgttatcc ggctgacctg
     8281 gaagcgtcca gttgagctcc agtgcgccgc cgagatttgc cagattagta tcgtgtccgg
     8341 cgataaacag cactgaagtg ggtaatgtca caccatacac ctgtttttgc ggtggatggg
     8401 gcgtcaacgc tgtcatgatc aaatccaaca acggggtggc gcggctgcgg gcaacctctg
     8461 gcgtgcgttg tagtaaataa aattgcgcgt tatgcaggct tagcaaggtg ttccactggt
     8521 gtgaatcggt gatccttccc caccccggct ccggcattcc ctgtgcttgt tgcaggagaa
     8581 atatctccgt cagcattgac gctaggctta ccgcaccggt taatgagaca ttgtcggcgc
     8641 tcaccttgag ttcagatggt aatgcctgcg ttaatgaaca actttcgtcc tgtttctcac
     8701 ggttaaggca caagtttgat tgtgggaaat taagcacctg ttccagttcg cgaaacgccg
     8761 tttgccgatg cccggtaaag tcagcaattg cccctcctgc cctgctgagg atcgcgtcag
     8821 tcacattcgc gttatccagt tggcaaacgc cagtttttag aggattaaat aacggatcgg
     8881 gactggacgt atctgcctgg gtatgtacgg ttattgcaca gtcaggtgcc agcccggcgg
     8941 cgaaggcttc gcctgtttta cgggtacgtt cgtcgacatc agcaataacc gcgacctgac
     9001 ctggctgcgg gcagcccttt tttgccagca atccgtcggc caccagacgc tggcgttggt
     9061 aatgtccgag ataagcgatt aactcaccac cgcgcggtgt cagccaaccc agttttaccg
     9121 gccaggttgg ccatgcgtca ggggtgacat cctgcatcag ttgcgtggcc ttggttggtg
     9181 cacgcacacc gtgacgactg acaatcacca cactttccag cttcagctcc ggctcactct
     9241 gagcgaatgc agatttcggt gttaacggaa tcagaagaga taagaatggg atcaagatcg
     9301 ctttcatcga tatgtttccg cttttcatat ccgacattat tgattgcctg atgcgacgct
     9361 aatgcgcctc atcaggctta ccttcgttaa cttttcaatc gcgggtccag ccataccaac
     9421 acaagggtgg agagcgaaca catcaacaaa atgccgacga accaaagtaa ataccacatt
     9481 gttcctgctc cttagtacag ctcgttttcg ttacggcgga gagtttctgt cgtcatgcgc
     9541 ccccacattt tgtagtagct ccagagagtg tagagcaaca caatgggcaa aaatatcagc
     9601 acgattacca acataatgct cagcgtcagc tgactggaag tgctgtccca caacgtcagg
     9661 ctggagatcg gactcacgct tgacggcatg acaaagggga acagcgtgat gcctgccgtg
     9721 aaaatcacgc caaattgcat caatgacgcc atcaaaaatc cccaacccgg gcgaccacga
     9781 taaatcgcca tcaccgtcag caatgggcag aagaacccca gcagcgggaa gatccacaaa
     9841 acgggcgatt cgacgaaatt gttcatccag gcaccaggta gcactgccac cagtttcatt
     9901 aacggattgg aaggaccgtt agcatcctgg gcgagcagta caaagccatc aataccaacc
     9961 cacagccagt aacccgccag caaaaagcac agcatcacca gtagtgcggc gcgtttggtc
    10021 gccagctgtg aacgcagatg aatcacacca acagttttca gttgtaacca gacgccacct
    10081 tgcaaaatca ccatcccaag actgagcaat ccacacaata aagggaatgg cgtcagcagt
    10141 tgccagaagc tgccgagata ttccacgcgt aattgcggtg tgaaagcaaa cggcacgccg
    10201 agcagcaaat tgccgaatgc aataccgaag actaccggcg gcaccagact gccgatgacc
    10261 agtccggcat cccacatttt acgccagcgt gcatcggcga tttttccgcg ataatcaaag
    10321 gccagcgggc ggaagaacag cgagcacagc accagaatca tcgccacata aaagccggaa
    10381 aacgccgctg catacactct gggccaggcg gcaaataatg ccccacctgc gaggatcaac
    10441 cagacctgat tgccttccca gtgcgcgcca acgctgttta tcactatccg acgttcatca
    10501 tcattacgcg ccaccagcgg cagcagacag ccgatcccca tgtcaaatcc gtcggagatc
    10561 ataaagacca ccaggatcac gccaatcagc agccaccaga tgaagcgcaa tgtttcataa
    10621 tcaaacatga ttttctcctt tacccctgtt gctgcgtcgg ttgttcactc tgcatcgcgc
    10681 tcggccccag acgggcatat ttctgcatca ggtagacttc ggcgattaag aacagggtgt
    10741 aaagccctac gatcatgatc agtgagaaag ccagttgtcc tgtggttaag gcggagtgcg
    10801 cggagtatgt cggtaagatg tcctgtatcg cccacggctg acgaccaaac tcggtcataa
    10861 accatccggc ttcaatcgca atccacggca gcggcaaact ccagagcgcc attttcagca
    10921 cccagcgatg ctggtcgatt ttgccacgca gcgtctgaac aagcgcaatc agcatcacca
    10981 gtagcagcag ggaaccacag cccaccatga tgcggaaact ccagaatacc ggcgcaacct
    11041 gaggtatcgc gccacgcatc gccgcctggt actgtgcggc tgtgacatga ttcatatccg
    11101 gtgcatagcg ggagagcaac atgccgtaac ccaggtcgcc ttccagctcc cggaatgcct
    11161 gcaacacatg cggctcacga ttgccttgcg atatttcctg cattaacagc cacgccatac
    11221 gtccgcgttg caagcgtggg taggtttcag ccatcaaatt cttcagaccc ggcacgggtt
    11281 tatctaatga gtgagtggcg aggatcccta gcagcgcggg aattttgatg gcaaaggcgt
    11341 tacgctcttg atcctgttcc ggccaggcaa ccacatggaa cggtgcaggt gcaggttctg
    11401 tttgccactc accttccatc gccgccagtt ttaccggttg tacttgcgcg acttcatacg
    11461 cagaactgtc tccgagttgc agcgtaccga taatcgccag agtaccgaag acggaaccga
    11521 tggcaaacga gcgtaatgcg acatcgcgct cccgtccgcg cagtaaatac caggcgctga
    11581 tcgccataat aaacatagcc ccggtcacgt atcccgccat tacggtgtgc acaaatttca
    11641 cctggctgac cgggttaaag accagctcgc tgaaactggt catctccata cgcagggtgt
    11701 cgatatcaaa atgcgcacca gtcgggtatt gcatccaacc gttggcattc aatatccaca
    11761 acgcagagag atttgaaccg aacgccacca gccacgtcac cagcaggtgc tggtatttat
    11821 tcagccgttg ccagccgaag aagaacagcc caacaaaggt ggattcgagg aagaaggcca
    11881 ttaatgcttc catcgccagt ggtgcgccaa aaatatcgcc cacatagttg gaatagaatg
    11941 accagttagt accaaactga aactccatcg tcaggccggt agccacgcca agagcaaagt
    12001 tgataccgaa gagcttaccc cagaagcgcg tcatatcgcg gtagattgtt ttgccggtga
    12061 ccacgtaaat ggtttccatg atagccagca aaaaaatcag ccccagggta aggggtacaa
    12121 ataaaaagtg atacagcgcg gtcagagcaa actgccagcg cgataaatca atgacatccc
    12181 acatgcgcac tcctgtaggc gaagagagaa aaataacccg ttaatggtag atctccctct
    12241 ttatcctgaa actctacgac cagaggtatt cgccgattgc gatattcata agcctgaaag
    12301 cgatacttac cgtcttttta cgtcggtgcc ccctccgcca gccactggca tacctcgcta
    12361 agccgctgcg cagagtcgac caaatcttct ggcgctgcca gcaccacttc cggtattggg
    12421 cagatttcat aactttccag taacgggcct tttaaggtgt ccgtacagcg taaatgccag
    12481 acatggcgta accccgtggc gttgatatag ctctcgccat agccaatggt acgaatctga
    12541 atatttcccg gcccacagag acgtgagaga aaaagccggt cagcttcgct gatgggtaat
    12601 tgcgtcagat taatgctgtg cggctgcgcg tcggggttac gtacatgtgc cagtaattca
    12661 tgggccaacg gtaggccatt catcaggcca tcaatgggcg gcggtaacag cgaatctgtc
    12721 ggcaagacgt tttgcgttgc cgcctgccac aacgccagcg gtgcgcagcc agcctccagt
    12781 ttgtcttcca gcaactgttc gccgtgacgt ctgcgtaccc gccataatcc gcagaagatc
    12841 gcctcctgaa tttcactttc actgtcgtca gcctgctgaa tgcgcactga cacttcccct
    12901 tccccgagaa gcgtattgat aaagtgacga tcgtcggcgt tgagatttaa cagatcgact
    12961 gccagtacct cacccagcgg cggctggcgt tcgcttagtt gatgttgtaa ctcgtttaac
    13021 agcgcaatca cctgcgggct gtgagcacat tgctccaggg ccgccatact cggttcacca
    13081 ttcacccgac aggtgatcgg cagtggattc atgctgaaac tgtcatcgtt cggttgcgtt
    13141 cctggcccca gcagatggaa aaaagtttcg ctcatgaggc acgctcctgt tgtggttcga
    13201 caagcccacg catcaggttt atcagttccg cccacgggtg aataccattc agcacgccgc
    13261 gatagtttcc gccggtaaac actaatgtgg cgggaaagcg aaagacgcca aagcgatcgc
    13321 cgatggcttc gctctgctca agatcagcaa tcgctacctg ccatgtatag tcgggaaact
    13381 cgcgcagtaa ttcgccaatc atgaccgggt tatcgctgac ttctggcgtg cgtttcgggt
    13441 cactacttaa taacaccacg ccgtctggcg cttgcgtaag ccagtcgtca agacggcatt
    13501 cactgaccgg cgtccagccg cgcgccagca ttcgttgcca caacgcatca aatggtgtgt
    13561 cgttgctcat ccctgttcct ccagcctaat cttgtactga cgcaagcgaa cgccttcgta
    13621 attttccatc gacagacagt cataattgag acagcgcaac tcctcagtcg gttgcggtac
    13681 aattccccag gccgccagct gtaccagcgc cgcctgttcc gcggcgggca gttgctcccg
    13741 cgccagttcg ctcaggctgc cgccgtagtc gtcgagcatc gcgggttgca aaccgacgag
    13801 ggcaatatgt gctggcagat gtccgcggat atctgccagc gccagcactt cggagaaact
    13861 gttctggtgc aggctcattt tcttcgcgct gagataagcc ggaatgcgtt ctccggcata
    13921 cgttcgtagc gttccgggtt ccagcccgta atcaatggca tcgagaatca acagatgact
    13981 ggcgctttcg acatacccca gcaagttcag tccttgggtg ccgccatcga caatctccac
    14041 atcctctggc cagtggtaat gggcatacag ccgttccgcc acccgcacgc cgaagccttc
    14101 atcggcccac agcaggttgc ccagccccat taccaccacg cgttgctcgc tcatgaacgc
    14161 tccttgttac ttattttgcc aaatttgtgg ctacggtagc cgttgaccat agtggagatc
    14221 accgtgtcgt cggacatgat gtcttcacgc agcgccatgt agacatgacc gatcacaaac
    14281 gcgccaatca gccacatccc cagccgatgc cagctgtgaa tgtccattga gttgccaccc
    14341 gtccagtaga aaaattccac cacataacgg aacggcgcaa aaatagcgta ctggctgtgt
    14401 tcgctgtaca gcgcaaagcc ggtgatgatc ataaaaactg acatcaggaa atagccgaac
    14461 atcgccgcct gggcgatggg gttatggcct atatcggcac tcggacgttt tgccagaaac
    14521 agataccagc ggatttcata ccacacgccc tgccaccagc ttttacgcca taccggcacg
    14581 ataaacagct cgcgggagta acgattgcca acaaaagccc agtagatccg catcagcaag
    14641 accacggtaa aaatcatccc ggcgctgaag tgaattaacc tgatgtagcc catatagaac
    14701 agatacgtcg cctcgccgct gacggaaggt agcggcttgc cgataaagta accggtgacc
    14761 atcaacaccg ccatgcataa cactgtcagc cagtgccata tccgcacggg tgcttcaaag
    14821 acatagtgac tgacaacgtt gtcgcttttc tgttgcatga tgattctcct tcgctgttaa
    14881 cgcacctgca cggagataag ctcgctaccg tcgtcgccca gcacgtgtgt tgaacaggcg
    14941 aggcacgggt caaagctgtg cagagtacgc aggatctcca gcggttgctc ggggatcgcc
    15001 attttggtgt tcatcagcgc cgcttcataa gcgccaatct gccctttggg atcgcgcggg
    15061 ctggcgttcc aggtggtcgg caccacgcac tggtagagat caatcttgcc atcgcgaatg
    15121 gcagcccagt ggcctaacgc cccgcgcggc gcttcggtaa aaccgacacc acggcactct
    15181 gtcggccagg ttgctggttc ccatttttcc gtggaagcgg tggcaagatt gccgtttttc
    15241 aggttggtca tcagtttatc gaagaaatac tgcaacttac ctgcggccca ctgcgcttcg
    15301 tgcgcgcgac acaaaatgcg gcccaacgtt gactggatgc cggaaagcgg cagtttcaac
    15361 gccgacatca tacgatcgac cgactcaacg gtcgcagcat cgcctttgtg ataagcgatt
    15421 aacgtgcgtg ccagcggccc cacttccatc gcgttaccgc gccagcgtgg cgctttgatc
    15481 cacgagtagc gttcctgttc attcagctgc tgaatgttgg tatcgctgcc tttgacatcg
    15541 ccggggttgt accacgggtc ggtaatgcca tcgaacggat gacgccccac ctgatcgttg
    15601 ggataacgat accaggcgtg gtcgacaaac tcctgcacct gctgcggatc aaccaaatcc
    15661 actggcagca cattgttgaa gtcgccgtta atcaccgcgc cgccaggcat cagcagactt
    15721 ttctcactaa agtcgttggc aatatccggg aatgcgccgt agctgaggac acatttatca
    15781 gaaagacccg tgccgatttc gctccacggt ttgttgaact ggccgatggc taaggcgtcg
    15841 gggatcatca cgttgttaat gaagtccgcc gtacgggtaa tgatcgactg caccaggttc
    15901 aggcgttcca tattgaccgc cccgactgcg ccgctttcgt caatgttaat ggcgcaaggc
    15961 atcccgccga caatccagtt tggatgcggg tttttaccac caaagaccgc gtggattttg
    16021 acaatttcac gctggaaatc gagagcttcg agatagtggg caaagcccat caggttggct
    16081 tctggcggca gtttgtactg cgggtgcccc cagtagccat tgcggaagat ccccaactgc
    16141 ccgccttcaa cgaatttctt caggcggttt tgtacgtcga agaaatagcc aggggatgat
    16201 ttcggccatg aggagaggct ttgcgccagt tcggaggttt tccgcgggtc ggctttcagc
    16261 gcatctaaca catcgatcca gtccatcccg gcaagctgat agaagtgcac cagatgatcg
    16321 tggcaccaga gcgttgccag cataatgttg cggatgatat tggcgttatc cggcacttta
    16381 ataccgatgg catcttcgat ggcgtaaacc gaagccaggg cgtgtacgcc agtacagacg
    16441 ccgcagatac gttcaacgaa cgcccacgca tcgcgcgggt cacgcccttg caggatgatc
    16501 tccagcccgc gaaacatggt gccgcaggag acggcattgg tgatcacatt ctgatcgtta
    16561 atattcactt cgcagcgcat gtggccttca atgcgcgtaa tcgggtcgac caccaggcgg
    16621 cgtccggcat tgttgatggt gtatccctga gtttcgtact gagtgctcat gcctgtttat
    16681 cctcattgcc tggctgatgt tcggtttctg taggttgctg gttatgacgt ctgcgctggt
    16741 caacggcgct ggcgactgcg tgcacaccaa cagccgctgc caccacgcca agcgcggtta
    16801 aaccgacggt gtcggcggtg gaatgggtac ccatttgtgg aatatcgacc acgcggctgt
    16861 agaacgaacc gcgatcccag aaaccatttt ccgcacaacc caggcagccg tgacctgact
    16921 ggatcgggaa ggaaacgcca tcgttccagc gtgtggagga acaggcgtta taggtggtag
    16981 gccctttgca gcccattttg tacaggcagt aacctttgcg ggcagcgtca tcatcccaac
    17041 tctggacgaa ctctccggcg tcgaagtgag cacggcgata gcatttatcg tggatacgct
    17101 gaccatagaa catcaacgga cggcccatcc tgtcgacatc cggcagacga tcaaaggtca
    17161 ccatgtaagt aatgatggcg ctcatcacat ccgggatcgg cgggcagcca ggtactttga
    17221 taataggttt gtcggtgatg actttgtcga taggcgttgc ctgcgtcgga ttgggtcgcg
    17281 cggcctgcac gcagccccag gacgcgcagg ttccccaggc gataatcgca ctggctccgg
    17341 cagcggcacg tttgagtttc tcaataaacg gtcgaccgct gctgatacag aacatcccct
    17401 gctcgcccag cggcggatta ccttcaacgg cgaggatata tttgccattg tattgcgtga
    17461 tgatgtcttc aaagacttct tccgcctggg ttccggcggc agccatcaaa gtatcgtcgt
    17521 aatcgaggga aatcagggaa aggatgacgt ccttcgccag tgggtgagcg gagcggataa
    17581 aagattcggt acagcaggtg cattcaagac cgtggatcca caccaccgga atgcgcggtt
    17641 tattctccag cgcccaggca atctttggtg ccattcccgc gcctaatccc agcgacgtgg
    17701 cagccagact acaatatttg agaaagctgc gccgggtaac gccctgacgc cgcatggcct
    17761 ggtaaaatgt ttcctcgtta ttcatatcgc acgtctcttc tccttgcgac accggcagga
    17821 caccgggcgc acatggtcgg caagcgacgt ccctgtagca aggcaaacca tcgcttcacg
    17881 tgagatgctg aaaacgaaag ctcatccttc tgcactggcg cacgtcgcca gaaagtattg
    17941 ttaataaagc gtagtgaaac ttttgcacaa aacaatacaa actgtgtgga tttatctttt
    18001 agcgataaaa atggacctat ttttcttttt gccgggcggt ggggatgttt agccagttgc
    18061 taaacgagta aaagagaagg aattcatggt gatgaaatga tcaatctgct tttatttcac
    18121 gctatcaatg cacttttggc tgtttttcag gcaaacaaac aaattagggg tttacacgcc
    18181 gcatcgggat gtttatagtg cgcgtcattc cggaagtgtg gccgagcggt tgaaggcacc
    18241 ggtcttgaaa accggcgacc cgaaagggtt ccagagttcg aatctctgcg cttccgccaa
    18301 ataagataag gggttagcta aatgctaacc cctttttctt ttgcctgtcg aaattctcag
    18361 ggcgttatat cactgcctaa tgacctgaga ctccgctgtg aaacttgttt cagatcaaat
    18421 gcgtaaagat gggtaaaact tctgtgtacc ttcccacatt atcattatgc tgcttaatta
    18481 attacatctg tcatagagag tgactcaatg gatcgtatag ttagttcttc acatgaccgt
    18541 acatcgctgc ttagcaccca taaggtgctg cgtaatacct attttctgct gagcctgacg
    18601 ctggcctttt cggcgattac cgcaactgcc agtacggtgc tgatgctgcc atctccgggt
    18661 ctgattctga cgctggtggg tatgtatggt ttgatgttcc tgacctacaa aacggcgaat
    18721 aagccgaccg ggattatctc cgcattcgcc tttaccggtt ttctgggtta tatcctcgga
    18781 ccaattctga atacctatct gtctgccgga atgggtgacg taatcgctat ggcactgggc
    18841 ggaacggcgt tagtgttctt ctgctgctct gcatatgtgc tgaccacccg caaagatatg
    18901 tcgttcctcg gcggtatgct gatggcgggt attgtggtgg tgctgattgg tatggttgct
    18961 aatatcttcc tgcaactgcc tgctctgcat ctggcgatca gcgcggtctt cattctgatc
    19021 tcctctggcg ctatcttgtt tgaaaccagc aacatcattc atggcggtga aacgaactat
    19081 attcgtgcca cggttagcct gtatgtttcg ctgtacaaca tcttcgtcag cctgctgagc
    19141 attctgggct tcgccagccg cgattaatct cacccgctaa cactttcata gcctcgcttt
    19201 atgcggggct ttgttttttg ttacactgcc gccagtctga ttaaggtagt aaagaattat
    19261 gctgatcttc gaaggtaaag agatagaaac ggataccgaa ggctatctca aagaaagcag
    19321 ccagtggagc gaaccactgg cggtggtgat tgcagagaac gaagggattg cgctgtcgcc
    19381 agaacactgg gaagtggtgc gttttgtgcg tgatttctat ctggaattca atacttctcc
    19441 ggcgattcgt atgctggtaa aagcgatggc gaataaattt ggcgaagaga aaggcaatag
    19501 ccgctatctg taccgactgt tcccgaaagg tccggcaaag caagccacca aaattgctgg
    19561 cctgcctaaa ccggtaaaat gcatttaacg gatacgaaaa tccgttaact ccccggaggg
    19621 atgatgcggc tcgctaagca cccgttccac acgcgctgaa cgcgggccgc cactttttaa
    19681 ccactgcatt aatttttcca cctgcccttc ttcaccgcag gcaaccactt ccacgctgcc
    19741 atcgtcaaga tttttggcgt acccggttag tcccagtttt ttcgcttcgt actgcgtggt
    19801 gtagcggaat cctacgccct gaactcgccc gtaaacccag gcaattatgc agactttcga
    19861 cattgctgct ctccttatcc ttaatggacg cgttgcaaat ccatggaaaa ctccggacaa
    19921 tgacgcccat ttctttgcat cgacagaaaa gtaaattatg agtgtacgtt tagtgttagc
    19981 caaagggcgc gaaaaatcat tacttcgtcg ccatccgtgg gtcttttccg gggccgttgc
    20041 ccgcatggaa ggtaaagcca gcctcggtga aaccatcgat attgttgatc atcagggaaa
    20101 atggttagca cgcggcgctt attcgccagc ttcgcaaatc cgggcgcgcg tctggacgtt
    20161 tgacccgtct gagtctatcg acattgcttt ttttacccgc cgtttacagc aagcacaaaa
    20221 atggcgtgac tggctggcgc aaaaagatgg cctcgacagc tatcgtttaa tcgccggaga
    20281 atctgatggc ctgccgggta ttactatcga tcgtttcggt aattttctgg tgctgcaact
    20341 gctgagtgct ggcgcagagt atcagcgcgc ggcattaatt agtgccctgc aaacgctgta
    20401 cccggaatgt gctatttacg atcgcagcga cgtcgcggta cgtaaaaaag aagggatgga
    20461 gctgactcag ggccccatca ccggcgagtt gccgcctgcc ctgctgccga ttgaagaaca
    20521 cggcatgaaa ctgctggtgg atattcagca cggacacaaa acgggctact acctggacca
    20581 gcgtgacagc cgcctggcta cccgccgcta cgtcgaaaat aaacgcgtac ttaactgttt
    20641 ctcctatacc ggtggtttcg ccgtatcggc attgatgggc ggttgcagcc aggttgtcag
    20701 cgttgatacc tcccaggaag cgctggatat tgcacggcag aacgttgagc tgaacaaact
    20761 ggatctgagc aaggctgagt ttgtccgtga tgatgtcttt aaattgctgc gtacctatcg
    20821 cgatcgcggt gaaaaatttg acgttatcgt gatggacccg ccaaagtttg ttgagaataa
    20881 aagccagttg atgggcgcgt gccgtggcta taaagacatc aacatgctgg cgattcagtt
    20941 gctgaatgaa ggcggtgttc tcctgacttt ctcctgttcc ggtctgatga ccagcgattt
    21001 atttcagaaa atcatcgcgg atgccgcaat tgatgccggt cgtgatgtac aatttataga
    21061 gcagttccgt caggcagccg atcatccggt gatcgctacc tacccggaag ggctatatct
    21121 gaaagggttt gcctgtcgcg tcatgtaact tgaaatgtgg aattttaccc ttatatcaaa
    21181 ggtaaagatt tcccgggagg tgactatgat tgccagcaaa ttcggtatcg gccagcaggt
    21241 ccgccattcc ctgttaggtt atctcggagt ggtcgtggat atcgacccgg tttattcgct
    21301 ctctgaaccg tcgcctgatg agctggcggt gaatgacgag cttcgcgccg ctccgtggta
    21361 tcacgtggtg atggaggatg ataacggcct accggttcat acctacctgg ctgaagccca
    21421 gctaagtagc gaattgcagg atgaacatcc tgaacagcca tccatggatg agctggcgca
    21481 gaccatccgc aaacaactcc aggccccgcg tctgcgtaac taatccctga acaaatcccc
    21541 ggaaggtttt tgccttccgg ggatttttta tttggccagg cccaggcgag ggatctcaat
    21601 cgccgggcag cgatccatca ccacgtttag ccctgcatca cgcgccagca ctgccgcttg
    21661 ctcattaatc acccccagtt gcatccagag ggttttcgcg ccaatggcga tagcttcctg
    21721 cgccacgccc cacgccgctt ctgaattacg gaaaacatcg accatatcga ctttttctgg
    21781 aacgtctgcc agcgtgccat acccctgctg gcccagcagt gttttgccgg caacttttgg
    21841 cgaaacagga atgacgtgat acccctggtc gagtagatat ttcatcacgc gatagctggg
    21901 acgatcgggt ttgtcgcttg cccccaccag cgcaatggtg tgagtggacg ttaaaatgcc
    21961 tgcaatatcg gtttctttca ttatttcctc cagactgttt aacaaagtgt acgacaaagc
    22021 cgacatgcca accatgcagc taatacgaat gaatgagagc aaaagcggga aatatgtcta
    22081 tatgttagta agtatgatta aagaaaaatt ttgacacatt attattcggg cggtctttta
    22141 acaggagcta tctatgaaaa ccggcatcgt gaccaccttg attgcattat gtttgccggt
    22201 atccgttttt gccaccacgc tgcggctttc tactgatgtc gatcttctgg tactcgacgg
    22261 taagaaggtt tccagctctc tgctgcgcgg tgcggacagc attgaactgg acaatggacc
    22321 gcatcagtta gtgtttcgcg ttgagaagac gattcatctt tccaatagtg aagaacggct
    22381 ctatatctcc cctccactgg tggttagttt taatacccag ctcatcaacc aggtcaattt
    22441 tcgcctgcct cgcctggaga atgagcggga agctaaccat tttgatgccg cgccgcgcct
    22501 tgaattgttg gatggcgatg cgacgccgat tccggtaaag ctggatattc tcgccattac
    22561 ctcaactgca aaaacgattg attatgaggt cgaggttgaa cgctataaca aatccgcgaa
    22621 acgcgcttca ctaccgcaat ttgccacgat gatggcagat gacagtacgc tgctttcggg
    22681 tgtttccgag ctggatgcta ttccaccgca gtctcaggtg ctcacagaac aacggctgaa
    22741 gtattggttt aaactggctg acccacaaac gcgaaatact ttcctgcaat gggcggaaaa
    22801 acaaccatcc tcctgagatt tttgtgcctg tgcgcaggct ttttcggtct ttatcttgca
    22861 gcgataagtg cttacagtaa tctgtaggaa agttaactac ggatgtacat tatggaactg
    22921 acgacacgca ctttacctgc gcggaaacat attgcgctgg tggcacacga tcactgcaaa
    22981 caaatgctga tgagctgggt ggaacggcat caaccgttac tggagcaaca cgtactgtat
    23041 gcaacaggca ctactggtaa cttgatttcc cgtgcaaccg gcatgaacgt caacgcgatg
    23101 ttgagtggcc caatgggggg tgaccagcag gttggcgcat tgatctcaga agggaaaatt
    23161 gatgtattga ttttcttctg ggacccacta aacgccgtgc cgcacgaccc tgacgtgaaa
    23221 gccttgctgc gtctggcgac ggtatggaac attccggtcg ccaccaacgt ggcaacagca
    23281 gacttcatta tccagtcgcc gcatttcaac gacgcggtcg atattctgat ccccgattat
    23341 cagcgttatc tcgcggaccg tctgaagtaa tattgcacag gtggcaaacg ccacctgttt
    23401 cttacggttt tctcgccacc ggcacatcca gatttttcag tatttcgaca aatggagagg
    23461 gattctcttt gttaaacagt gcccataccc gatggcgtgc ccgggtcagc gccacgtaca
    23521 ttaaccgccg ttcttcagcg tccgggaaat cctcgaccgg tggcagtagc gcctcttcca
    23581 taatcgactc ccgcgccgca gccggaaaac catcactccc ctcctgcaag ccaacgatga
    23641 tgacgtaatc cgcctgttgc cctttgctgg catgaatggt cataaagtcg atttgcaact
    23701 tcggccagcg tgttgccgct ttttccaggc tggcaggcct catgtgatgg taacgcgcca
    23761 ggatcagaat gcgctcttcc ggtttggcat aaccagagag cttatccagc aaagcgtcaa
    23821 gatggctctc atccaataac gtgacggctt ttttgtctcc attggttaag ctgtttagcg
    23881 gctttttcag ctggccaggg ttctgctgaa taaaccggtt tgccacctca ccgatacgac
    23941 tgttaaaacg gtaagtcgtg tctaaatcgc agcgatcacc ttcaccaaag ttttcatgga
    24001 aagcggtggt gagcgacatt tgtgcaccgc tgaagcgata aatcgcctgc cagtcatcac
    24061 caacggcgaa caacgtcgtc tgactgtttt gcttgcgtaa tgccgctaac aacgctgcgc
    24121 gctgcggcga gatatcctga aattcatcaa ccagaatatg cttccacggg ctgataaagc
    24181 gacctttctc cagaatcaca attgcctggt gaataaggcc cgaaaaatcg acagcgtttt
    24241 ctgccttcag cgcacctttc caggctttta ataacggagc catcaacttg atacgtttac
    24301 tgaacagatc gcgaatctct tcgggtgcac tggcaatcat ttctgcctgt gcaccaccgt
    24361 gcatccgcat cagacttacc caacgatcga ggcgtgaggc aagacgacgc tgtaattttt
    24421 catcatccca gaagttacct tctggcactg accactgcat ttcttccgtc agccattgcc
    24481 gccagccctt cgcctgcgct ttcttttcgc tgcattgctt gcaccactca gcaataaaaa
    24541 gttcatgacg ggcagcggta tcattttcca gtttgctgac tatcggaact tttttgctac
    24601 cctgttgaat aatatgcagc gccagcgcat gaaatgtgcg tgcggtaatg tcttcggtat
    24661 gtagtcgttc tcgaatacgc tcgtccatct cttcagcggc tttgcgacca aacgccagca
    24721 ataaaatttg ctcaggggac gcttcaccac gcgccagtaa ccagccagca cgggccacca
    24781 gcaccgacgt ttttccgctt cctgcacctg ccagcactaa cagagaatgc tcgccattaa
    24841 cgactgcccg ggcctgcgcc ggattcagcg gtgaagactc gacctggcgg aaaaaatccg
    24901 catactcggt aagcatggct tcggtatacg cctgattatg ctgcaaccga gcgccttcaa
    24961 tgtccttcaa ccaggcctga catttacgcc acgcctcacg gcagttatcg aattcttcca
    25021 gtcggttaac cggcaatggt aacgccgaca aagcctggcg gatttgttgc tgcacgccag
    25081 aggtttgctc acgcgtcagc catttatttt ctccggtgcg cgtggcaatc aaatccagtt
    25141 gctgacgtaa aacgccagac gcaatttcgc tcatctcgct actccaccgc cgccagtgag
    25201 catcaagatg atggtaaaaa cgctgcgtct cgccccattc ggtgccgtgc agacgcacca
    25261 ctttttcgtc cggcagtaca aattccagct cgccccatac cagaccgcgc ttacagtgaa
    25321 tcgccagtaa ttgattgaaa ggaataaggt attcgtggcg atcgccggag actttaatcc
    25381 cggcattgag gatcaccgcc cgatcgtaag ggtgctgtgc cagacgtttt ccaagcgttg
    25441 tcgctttcag ttccatgact ctgtagatcc acacgaaatg atttcttatc agtgtaaccg
    25501 tcagagaaaa cgtgctccag cccaaaaaaa cgttacaatt gccgcccatt actgaaaacc
    25561 acagtaaagc gaggttttat gcgtaccgtt ttgaacattc tgaactttgt gcttggcgga
    25621 tttgccacca ctctgggctg gctgttggcg actctggtca gtattgtgct gatttttacc
    25681 ttaccgctga cacgatcctg ctgggagatc actaaactgt ctctggtgcc ttatggcaat
    25741 gaagctattc atgtcgatga actgaacccg gcgggcaaaa atgtgctgct gaatactggc
    25801 ggtacggtat tgaatatttt ctggctgatt ttctttggct ggtggttatg cctgatgcac
    25861 attgcaacgg gcatcgcaca atgtatttcc atcattggca ttcctgtcgg cattgcgaac
    25921 tttaaaattg ccgctattgc actatggccg gtcggtcgtc gcgtggtatc ggtagaaaca
    25981 gcgcaagctg cgcgtgaagc caatgcacgt cgtcgttttg aataatccac taatatggcc
    26041 tttatgctaa gtcctttgct caaacgctat acctggaaca gcgcctggct gtattacgcg
    26101 cgtattttta ttgcgctttg tggaaccaca gcgtttccgt ggtggctggg tgatgtaaaa
    26161 ctgacgattc cgctgacgct ggggatggtg gcagcggcgc tgaccgatct cgatgaccga
    26221 ctggcgggac gtttgcgtaa cctcatcatt acgctgttct gcttttttat cgcctcgacc
    26281 tcagtagaac tgctgtttcc ctggccctgg ctatttgcga ttggcttaac gctctcaacc
    26341 agcggcttca ttttgctcgg cggtctgggt caacgctatg caacaattgc cttcggtgcg
    26401 ttactgatcg ccatttacac tatgttgggg acgtcactgt atgagcactg gtatcagcag
    26461 ccgatgtatc tgctggccgg tgccgtctgg tacaacgtcc tgacacttat tggtcatcta
    26521 ctgttcccgg tccgcccgct gcaggacaac ctggcgcgtt gctatgaaca actggcgcgt
    26581 taccttgagc tcaagtcgcg catgtttgat cctgatattg aagatgaaag ccaggcaccg
    26641 ctgtacgatt tggctctcgc caacggtcag ctgatggcga cattgaatca gacgaaactc
    26701 tcgctgctga cccgcttacg tggcgatcgt ggtcaacggg gaacgcgtcg cacgctgcat
    26761 tattactttg tcgcgcagga tattcacgag cgtgccagct cttctcatat tcagtatcaa
    26821 acattgcgtg aacattttcg ccacagcgac gtgctgttcc gttttcagcg gctgatgtcg
    26881 atgcagggcc aggcgtgcca gcaactgtca cgctgtattt tgttgcgtca gccttatcaa
    26941 catgatccgc atttcgagcg cgcttttacg catattgatg ctgcgctgga gcggatgcgc
    27001 gataacggcg cacctgccga tttactcaaa acactgggat ttttgctgaa caatttacgc
    27061 gctattgatg cccaactggc aacaattgaa tcagaacagg cccaggcact gccccataat
    27121 aatgacgaaa atgagctcgc tgatgacagc ccgcacggat tgagtgatat ctggctgcgt
    27181 cttagccgtc acttcacgcc ggaatccgcc ctcttccgtc atgcggtaag aatgtcgctg
    27241 gtgttgtgct tcggctacgc catcattcag ataaccggaa tgcatcacgg gtattggatc
    27301 ttgctgacaa gtttgtttgt ctgccagcca aactataacg ccacgcgcca ccggctgaag
    27361 ttaaggatta ttggtacgct ggtaggtatc gcaattggta ttcctgtgct gtggtttgtg
    27421 ccatcactgg aagggcagct ggtgctgctg gttattaccg gcgtgctctt ttttgccttc
    27481 cgtaacgtgc aatacgccca tgcaacgatg ttcatcacac ttttggtgct actgtgtttc
    27541 aacttgctgg gtgaaggttt tgaagtggcg ttacctcgcg taatcgatac gctgattggt
    27601 tgtgccattg cgtgggcggc agtgagctac atctggcctg actggaagtt tcgcaatctg
    27661 ccgcgaatgc tcgaacgcgc cacagaggcc aactgccggt atctagatgc catactggaa
    27721 caataccatc aggggcgtga taaccgtctg gcgtatcgta ttgcccgccg cgatgcacac
    27781 aaccgtgatg ctgaactggc gtcggtggta tcaaatatgt ccagcgaacc gaacgttacc
    27841 ccgcaaattc gcgaagccgc gtttcggttg ctgtgcctta accatacgtt taccagctat
    27901 atctcagccc tcggtgctca ccgggagcag ttaactaatc ctgaaattct ggcgtttctt
    27961 gatgacgccg tttgctatgt tgatgacgcg ctacatcatc aacctgctga tgaagaacgc
    28021 gtcaatcagg cattagctgg cctgaaacag cggatgcagc aacttgaacc acgggcagac
    28081 agcaaagaac ctctggtcgt acaacaagtt gggttattga ttgcattact gccagagatt
    28141 ggtcgtctgc aacgccagat tactcaagtt ccgcaggaaa ctcctgtttc ggcgtaagag
    28201 agtcagccca ttctgcaagc tcctggcggc gtgccaccgg gagcgcagct tcatgaatgc
    28261 cgataatggc cccttcaagc ataaacagaa tcttttctgt caccagactg ttttgctgcc
    28321 gcagtcgcaa ccaacacatt tttgccccaa gtatacgtaa cgcccgtaca tccttaatcc
    28381 ccacctcccc gagaatcgct tccagatgaa aagacatatt gggcaaatct ttcagtcttt
    28441 cccgaatatt gcgcgtgctt ttctctttca gcgctgcatc gagagaatat ttcgacagac
    28501 gcaccagctt cagttgattt cgccatagac tttcatcaac ccgatagtaa ttgagggtaa
    28561 cggatcggcc acacttttta tatgtcagcc agacaggcgg atgttttaca cagtacggtg
    28621 cactttgctc acaagcccga agatacaact caccatcaga aaccatcgca aacaccgtgt
    28681 cgtcaacggt taggctgtaa ctaccaaaca atgatcggta ttcaattgtg cccaacgttg
    28741 ccaggtattc ttgtgattta tagatccgct tataggagag gcttttcata aaattccttt
    28801 taaaataata acataaaaga atga
//
LOCUS       DADSYM010000054        28154 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2415
            SAMN08039579-rid11723173.denovo.054, whole genome shotgun sequence.
ACCESSION   DADSYM010000054 DADSYM010000000
VERSION     DADSYM010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039579
            Sequence Read Archive: SRR6362533
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 28154)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28154)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980162.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 03:39:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,484
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 4,347
            CDSs (with protein)               :: 4,347
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 43 of 85
            Pseudo Genes (incomplete)         :: 37 of 85
            Pseudo Genes (internal stop)      :: 19 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28154
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039579-rid11723173.denovo.054"
                     /strain="LGE2415"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8302 N 95.9628 W"
                     /collection_date="2015-08-24"
     gene            complement(18..863)
                     /gene="araC"
                     /locus_tag="J1Y83_001953"
     CDS             complement(18..863)
                     /gene="araC"
                     /locus_tag="J1Y83_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose operon transcriptional regulator AraC"
                     /protein_id="HBA7630102.1"
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKG
                     YILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHSEAHEWYHQWVYFRPRA
                     YWHEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLL
                     LRRMEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQ
                     LGISVLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEF
                     RAGCE"
     gene            1202..2902
                     /gene="araB"
                     /locus_tag="J1Y83_001954"
     CDS             1202..2902
                     /gene="araB"
                     /locus_tag="J1Y83_001954"
                     /EC_number="2.7.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulokinase"
                     /protein_id="HBA7630103.1"
                     /translation="MAIAIGLDFGSDSVRALAVDCATGEEIATSVEWYPRWQNGQFCD
                     APNNQFRHHPRDYIESMEAALKTVLAELSVEQRAAVVGIGVDTTGSTPAPIDADGNVL
                     ALRPEFAENPNAMFVLWKDHTAVEEAEEITRLCHTPGNVDYSRYIGGIYSSEWFWAKI
                     LHVTRQDNAVAQSAASWIELCDWVPALLSGTTRPQDIRRGRCSAGHKSLWHESWGGLP
                     PASFFDELDPILNRHLPSPLFTETWTADIPVGTLCPEWAQRLGLPESVVISGGAFDCH
                     MGAVGAGAQPNALVKVIGTSTCDILIADKQSVGERAVKGICGQVDGSVVPGFIGLEAG
                     QSAFGDIYAWFGRVLGWPLEQLAAQHPELKEQINASQKQLLPALTEAWAKNPSLDHLP
                     VVLDWFNGRRTPNANQRLKGVITDLNLATDAPLLFGGLIAATAFGARAIMECFTDQGI
                     AVNNVMALGGIARKNQVIMQACCDVLNRPLQIVASDQCCALGAAIFAAVAAKVHADIP
                     SAQQKMASAVEKTLQPRSEQAQRFEQLYRRYQQWAISAEQHYLPTSAPAQAAQAVPTL
                     "
     gene            2913..4415
                     /gene="araA"
                     /locus_tag="J1Y83_001955"
     CDS             2913..4415
                     /gene="araA"
                     /locus_tag="J1Y83_001955"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HBA7630104.1"
                     /translation="MTIFDNYEVWFVIGSQHLYGPETLRQVTQHAEHVVNALNTEAKL
                     PCKLVLKPLGTTPDEITAICRDANYDDRCAGLVVWLHTFSPAKMWINGLTMLNKPLLQ
                     FHTQFNAALPWDSIDMDFMNLNQTAHGGREFGFIGARMRQQHAVVTGHWQDKQAHERI
                     GSWMRQAVSKQDTRHLKVCRFGDNMREVAVTDGDKVAAQIKFGFSVNTWAVGDLVQVV
                     NSISDGDVNALVDEYESCYTMTPATQIHGEKRQNVLEAARIELGMKRFLEQGGFHAFT
                     TTFEDLHGLKQLPGLAVQRLMQQGYGFAGEGDWKTAALLRIMKVMSTGLQGGTSFMED
                     YTYHFEKGNDLVLGSHMLEVCPSIAVEEKPILDVQHLGIGGKDDPARLIFNTQTGPAI
                     VASLIDLGDRYRLLVNCIDTVKTPHSLPKLPVANALWKAQPDLPTASEAWILAGGAHH
                     TVFSHALNLNDMRQFAEMHDIEITVIDNDTRLPAFKNALRWNEVYYGFRR"
     gene            4615..5310
                     /gene="araD"
                     /locus_tag="J1Y83_001956"
     CDS             4615..5310
                     /gene="araD"
                     /locus_tag="J1Y83_001956"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HBA7630105.1"
                     /translation="MLEDLKRQVLEANLALPKHNLVTLTWGNVSAVDRGRGVLVIKPS
                     GVDYSIMTADDMVVVSIETGEVVEGTKKPSSDTPTHRLLYQAFPSIGGIVHTHSRHAT
                     IWAQAGQSIPATGTTHADYFYGTIPCTRKMTDAEINGEYEWETGNVIVETFEKQGIDA
                     AQMPGVLVHSHGPFAWGKNAEDAVHNAIVLEEVAYMGIFCRQLAPQLPDMQQTLLDKH
                     YLRKHGAKAYYGQ"
     gene            5385..7736
                     /gene="polB"
                     /locus_tag="J1Y83_001957"
     CDS             5385..7736
                     /gene="polB"
                     /locus_tag="J1Y83_001957"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase II"
                     /protein_id="HBA7630106.1"
                     /translation="MAQAGFILTRHWRDTPQGTEVSFWLATDNGPLQVTLAPQESVAF
                     IPADQVPRAQYILQGEQGFRLTPLALKDFHRQPVYGLYCRAHRQLMNYEKRLREGGVT
                     VYEADVRPPERYLMERFITSPVWVEGDMRNGAIVNARLKPHPDYRPPLKWVSIDIETT
                     RHGELYCIGLEGCGQRIVYMLGPENGDASALDFELEYVASRPLLLEKLNAWFATHDPD
                     VIIGWNVVQFDLRMLQKHAERYRIPLRLGRDNSELEWREHGFKNGVFFAQAKGRLIID
                     GIEALKSAFWNFSSFSLETVAQELLGEGKSIDNPWDRMDEIDRRFAEDKPALATYNLK
                     DCELVTQIFHKTEIMPFLLERATVNGLPVDRHGGSVAAFGHLYFPRMHRAGYVAPNLG
                     EVPPHASPGGYVMDSRPGLYDSVLVLDYKSLYPSIIRTFLIDPVGLVEGMAQPDPEHS
                     TEGFLDAWFSREKHCLPEIVTNIWHGRDEAKRQGNKPLSQALKIIMNAFYGVLGTTAC
                     RFFDPRLASSITMRGHQIMRQTKTLIEALGYDVIYGDTDSTFVWLKGAHSEEEAAKIG
                     RALVQHVNVWWAETLQKQRLTSALELEYETHFCRFLMPTIRGADTGSKKRYAGLIQEG
                     DKQRMVFKGLETVRTDWTPLAQQFQQELYLRIFRNEPYQEYVRETIDKLMAGELDTQL
                     VYRKRLRRPLSEYQRNVPPHVRAARLADEENHKRGRPLQYQNRGTIKYVWTTNGPEPL
                     DYQRSPLDYEHYLTRQLQPVAEGILPFIEDNFATLMTGQLGLF"
     gene            7901..10807
                     /gene="rapA"
                     /locus_tag="J1Y83_001958"
     CDS             7901..10807
                     /gene="rapA"
                     /locus_tag="J1Y83_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001117023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-associated protein RapA"
                     /protein_id="HBA7630107.1"
                     /translation="MPFTLGQRWISDTESELGLGTVVAVDARTVTLLFPSTGENRLYA
                     RSDSPVTRVMFNPGDTITSHDGWQMQVEEVKEENGLLTYIGTRLDTEESGVALREVFL
                     DSKLVFSKPQDRLFAGQIDRMDRFALRYRARKYSSEQFRMPYSGLRGQRTSLIPHQLN
                     IAHDVGRRHAPRVLLADEVGLGKTIEAGMILHQQLLSGAAERVLIIVPETLQHQWLVE
                     MLRRFNLRFALFDDERYAEAQHDAYNPFDTEQLVICSLDFARRSKQRLEHLCEAEWDL
                     LVVDEAHHLVWSEDAPSREYQAIEQLAEHVPGVLLLTATPEQLGMESHFARLRLLDPN
                     RFHDFAQFVEEQKNYRPVADAVAMLLAGNKLSNDELNMLGEMIGEQDIEPLLQAANSD
                     SEDAQSARQELVSMLMDRHGTSRVLFRNTRNGVKGFPKRELHTIKLPLPTQYQTAIKV
                     SGIMGARKSAEDRARDMLYPERIYQEFEGDNATWWNFDPRVEWLMGYLTSHRSQKVLV
                     ICAKAATALQLEQVLREREGIRAAVFHEGMSIIERDRAAAWFAEEDTGAQVLLCSEIG
                     SEGRNFQFASHMVMFDLPFNPDLLEQRIGRLDRIGQAHDIQIHVPYLEKTAQSVLVRW
                     YHEGLDAFEHTCPTGRTIYDSVYNDLINYLASPDETEGFDDLIKNCREQHEALKAQLE
                     QGRDRLLEIHSNGGEKAQALAESIEEQDDDTNLIAFAMNLFDIIGINQDDRGDNMIVL
                     TPSDHMLVPDFPGLSEDGITITFDREVALAREDAQFITWEHPLIRNGLDLILSGDTGS
                     STISLLKNKALPVGTLLVELIYVVEAQAPKQLQLNRFLPPTPVRMLLDKNGNNLAAQV
                     EFETFNRQLNAVNRHTGSKLVNAVQQDVHAILQLGEAQIEKSARALIDAARNEADEKL
                     SAELSRLEALRAVNPNIRDDELTAIESNRQQVMESLDQAGWRLDALRLIVVTHQ"
     gene            10819..11478
                     /locus_tag="J1Y83_001959"
     CDS             10819..11478
                     /locus_tag="J1Y83_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA pseudouridine(32)
                     synthase/ribosomal large subunit pseudouridine synthase
                     RluA"
                     /protein_id="HBA7630108.1"
                     /translation="MGMENYNPPQDPWLIVLYQDDHIMVVNKPSGLLSVPGRLEEHKD
                     SVMTRIQRDYPQAESVHRLDMATSGVIVVALTKAAERELKRQFREREPKKQYVARVWG
                     HPSPAEGLVDLPLICDWPNRPKQKVCYETGKPAQTEYEVVEYAADNTARVVLKPITGR
                     SHQLRVHMLALGHPILGDRFYASPEARAMAPRLLLHAEMLTITHPAYGNSMTFKAPAD
                     F"
     gene            complement(11596..12411)
                     /gene="djlA"
                     /locus_tag="J1Y83_001960"
     CDS             complement(11596..12411)
                     /gene="djlA"
                     /locus_tag="J1Y83_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone DjlA"
                     /protein_id="HBA7630109.1"
                     /translation="MQYWGKIIGVAVALIMGGGFWGVVLGLLIGHMFDKARSRKMAWF
                     ANQRERQALFFATTFEVMGHLTKSKGRVTEADIHIASQLMDRMNLHGASRTAAQNAFR
                     VGKSDNYPLREKMRQFRSVCFGRFDLIRMFLEIQIQAAFADGSLHPNERAVLYVIAEE
                     LGISRAQFDQFLRMMQGGAQFGGGYQQQSGGGNWQQAQRGPTLEDACNVLGVKPTDDA
                     TTIKRAYRKLMSEHHPDKLVAKGLPPEMMEMAKQKAQEIQQAYELIKQQKGFK"
     gene            12666..15020
                     /gene="lptD"
                     /locus_tag="J1Y83_001961"
     CDS             12666..15020
                     /gene="lptD"
                     /locus_tag="J1Y83_001961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly protein LptD"
                     /protein_id="HBA7630110.1"
                     /translation="MKKRIPTLLATMIATALYSQQGLAADLASQCMLGVPSYDRPLVQ
                     GDTNDLPVTINADHAKGDYPDDAVFTGSVDIMQGNSRLQADEVQLHQKEAPGQPEPVR
                     TVDALGNVHYDDNQVILKGPKGWANLNTKDTNVWEGDYQMVGRQGRGKADLMKQRGEN
                     RYTILDNGSFTSCLPGSDTWSVVGSEIIHDREEQVAEIWNARFKVGPVPIFYSPYLQL
                     PVGDKRRSGFLIPNAKYTTTNYFEFYLPYYWNIAPNMDATITPHYMHRRGNIMWENEF
                     RYLSQAGAGLMELDYLPSDKVYKDEHPNDDSSRRWLFYWNHSGVMDQVWRFNVDYTKV
                     SDPSYFNDFDNKYGSSTDGYATQKFSIGYAVQNFNATVSTKQFQVFSEQNTSSYSAEP
                     QLDVNYYQNDVGPFDTRIYGQAVHFVNTRDDMPEATRVHLEPTINLPLSNNWGSINTE
                     AKLLATHYQQTNLDWYNSRNTTRLAESANRVMPQFKVDGRMVFERDMEMLAPGYTQTL
                     EPRAQYLYVPYRDQSKIYNYDSSLLQSDYSGLFRDRTYGGLDRIASANQVTTGVTSRI
                     YDDAAVERFNISVGQIYYFTESRTGDDNITWENDDKTGSLVWAGDTYWRISDRWGLRG
                     GIQYDTRLDNVATSNSSIEYRRDEDRLVQLNYRYASPEYIQATLPKYYSTAEQYKNGI
                     SQVGAVASWPIADRWSIVGAYYYDTNANKQADSMLGVQYSSCCYAIRVGYERKLNGWD
                     NDKQHAVYDNAIGFNIELRGLSSNYGLGTQEMLRSNILPYQNTL"
     gene            15073..16359
                     /gene="surA"
                     /locus_tag="J1Y83_001962"
     CDS             15073..16359
                     /gene="surA"
                     /locus_tag="J1Y83_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase SurA"
                     /protein_id="HBA7630111.1"
                     /translation="MKNWKTLLLGIAMIANTSFAAPQVVDKVAAVVNNGVVLESDVDG
                     LMQSVKLNAAQARQQLPDDATLRHQIMERLIMDQIILQMGQKMGVKISDEQLDQAIAN
                     IAKQNNMTLDQMRSRLAYDGLNYNTYRNQIRKEMIISEVRNNEVRRRITILPQEVESL
                     AQQVGNQNDASTELNLSHILIPLPENPTSDQVNEAESQARAIVDQARNGADFGKLAIA
                     HSADQQALNGGQMGWGRIQELPGIFAQALSTAKKGDIVGPIRSGVGFHILKVNDLRGE
                     SKNISVTEVHARHILLKPSPIMTDEQARVKLEQIAADIKSGKTTFAAAAKEFSQDPGS
                     ANQGGDLGWATPDIFDPAFRDALTRLNKGQMSAPVHSSFGWHLIELLDTRNVDKTDAA
                     QKDRAYRMLMNRKFSEEAASWMQEQRASAYVKILSN"
     gene            16359..17348
                     /gene="pdxA"
                     /locus_tag="J1Y83_001963"
     CDS             16359..17348
                     /gene="pdxA"
                     /locus_tag="J1Y83_001963"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HBA7630112.1"
                     /translation="MVKTQRVVITPGEPAGIGPDLIVQLAQREWPVELVVCADATLLT
                     DRAAMLGLPLTLRPYSPNSPAQPQTAGTLTLLPVALRESVTVGQLAVENGHYVVETLA
                     RACDGCLNGEFAALITGPVHKGVINDAGIPFTGHTEFFEERSQAKKVVMMLATEELRV
                     ALATTHLPLRDIADAITPALLHEVIAILHHDLRTKFGIAEPRILVCGLNPHAGEGGHM
                     GTEEIDTIIPVLNELREQGMKLNGPLPADTLFQPKYLDNADAVLAMYHDQGLPVLKYQ
                     GFGRGVNITLGLPFIRTSVDHGTALELAGRGKADVGSFITALNLAIKMIVNTQ"
     gene            17345..18166
                     /gene="rsmA"
                     /locus_tag="J1Y83_001964"
     CDS             17345..18166
                     /gene="rsmA"
                     /locus_tag="J1Y83_001964"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HBA7630113.1"
                     /translation="MNNRVHQGHLARKRFGQNFLNDQFVIDSIVSAINPQKGQAMVEI
                     GPGLAALTEPVGERLDQLTVIELDRDLAARLQTHPFLGPKLTIYQQDAMTFNFGELAA
                     KMGQPLRVFGNLPYNISTPLMFHLFSYTDAIADMHFMLQKEVVNRLVAGPNSKAYGRL
                     SVMAQYYCNVIPVLEVPPSAFTPPPKVDSAVVRLVPHATMPHPVKDVRVLSRITTEAF
                     NQRRKTIRNSLGNLFSVEVLTGMGIDPAMRAENISVAQYCQMANYLAENAPLQES"
     gene            18169..18546
                     /gene="apaG"
                     /locus_tag="J1Y83_001965"
     CDS             18169..18546
                     /gene="apaG"
                     /locus_tag="J1Y83_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Co2+/Mg2+ efflux protein ApaG"
                     /protein_id="HBA7630114.1"
                     /translation="MINSPRVCIQVQSVYIEAQSSPDNERYVFAYTVTIRNLGRAPVQ
                     LLGRYWLITNGNGRETEVQGEGVVGVQPLIAPGEEYQYTSGAIIETPLGTMQGHYEMI
                     DENGVPFSIDIPVFRLAVPTLIH"
     gene            18553..19401
                     /gene="apaH"
                     /locus_tag="J1Y83_001966"
     CDS             18553..19401
                     /gene="apaH"
                     /locus_tag="J1Y83_001966"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase
                     (symmetrical)"
                     /protein_id="HBA7630115.1"
                     /translation="MATYLIGDVHGCYDELIALLHKVEFTPGKDTLWLTGDLVARGPG
                     SLDVLRYVKSLGDSVRLVLGNHDLHLLAVFAGISRNKPKDRLTPLLEAPDADELLNWL
                     RRQPLLQIDEEKKLVMAHAGITPQWDLQTAKECARDVEAVLSSDSYPFFLDAMYGDMP
                     NNWSPELRGLGRLRFITNAFTRMRFCFPNGQLDMYSKESPEEAPAPLKPWFAIPGPVA
                     EEYNIAFGHWASLEGKGTPEGIYALDTGCCWGGTLTCLRWEDKQYFVQPSNRHKDMGE
                     GEAAAS"
     gene            complement(19398..19712)
                     /locus_tag="J1Y83_001967"
     CDS             complement(19398..19712)
                     /locus_tag="J1Y83_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid maintenance protein CcdB"
                     /protein_id="HBA7630116.1"
                     /translation="MQFTVYRSRGRNAAFPFVIDVTSDIIGEINRRIVIPLTPIERFS
                     RIRPPERLNPILLLIDGKEYVLMTHETATVPVNALGTKFCDASAHRTLIKGALDFMVD
                     GI"
     gene            complement(19715..19948)
                     /locus_tag="J1Y83_001968"
     CDS             complement(19715..19948)
                     /locus_tag="J1Y83_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HBA7630117.1"
                     /translation="MTAKRTTQSVTVTVDRELVNRARDAGLNMSATLTVALNAELKKH
                     AATRWREENAEAIAALNQLADETGCFSDEYRSF"
     gene            complement(20034..20513)
                     /gene="folA"
                     /locus_tag="J1Y83_001969"
     CDS             complement(20034..20513)
                     /gene="folA"
                     /locus_tag="J1Y83_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020456510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 dihydrofolate reductase"
                     /protein_id="HBA7630118.1"
                     /translation="MISLIAALAVDRVIGMENAMPWNLPADLAWFKRNTLNKPVIMGR
                     HTWESIGRPLPGRKNIILSSQPGTDDRVTWVKSVDEAIAACGDVPEIMVIGGGRVYEQ
                     FLPKAQKLYLTHIDAEVEGDTHFPDYEPDDWESVFSEFHDADAQNSHSYCFEILERR"
     gene            complement(20705..22567)
                     /gene="kefC"
                     /locus_tag="J1Y83_001970"
     CDS             complement(20705..22567)
                     /gene="kefC"
                     /locus_tag="J1Y83_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     protein KefC"
                     /protein_id="HBA7630119.1"
                     /translation="MDSHTLIQALIYLGSAALIVPIAVRLGLGSVLGYLIAGCIIGPW
                     GLRLVTDAESILHFAEIGVVLMLFIIGLELDPQRLWKLRAAVFGGGALQMVICGGLLG
                     LFCMLLGLRWQVAELIGMTLALSSTAIAMQAMNERNLMVTQMGRSAFAVLLFQDIAAI
                     PLVAMIPLLAASSASTTMGAFVLSALKVAGALVLVVLLGRYVTRPALRFVARSGLREV
                     FSAVALFLVFGFGLLLEEVGLSMAMGAFLAGVLLASSEYRHALESDIEPFKGLLLGLF
                     FIGVGMSIDFGTLLENPLRIVILLLGFLIIKIAMLWLIARPLQVPNKQRRWFAVLLGQ
                     GSEFAFVVFGAAQMANVLEPEWAKSLTLAVALSMAATPILLVILNRLEQSSTEEAREA
                     DEIDEEQPRVIIAGFGRFGQITGRLLLSSGVKMVVLDHDPDHIETLRKFGMKVFYGDA
                     TRMDLLESAGAAKAEVLINAIDDPQTNLQLTEMVKEHFPHLQIIARARDVDHYIRLRQ
                     AGVEKPERETFEGALKTGRLALESLGLGPYEARERADVFRRFNIQMVEEMAMVENDTK
                     ARAAVYKRTSAMLSEIITEDREHLSLIQRHGWQGTEEGKHTGNMADEPETKPSS"
     gene            complement(22560..23090)
                     /gene="kefF"
                     /locus_tag="J1Y83_001971"
     CDS             complement(22560..23090)
                     /gene="kefF"
                     /locus_tag="J1Y83_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     oxidoreductase KefF"
                     /protein_id="HBA7630120.1"
                     /translation="MILIIYAHPYPHHSHANKRMLEQARTLEGVEIRSLYQLYPDFNI
                     DIAAEQEALSRADLIVWQHPMQWYSIPPLLKLWIDKVFSHGWAYGHGGTALHGKHLLW
                     AVTTGGGESHFEIGAHPGFDVLSQPLQATAIYCGLNWLPPFAMHCTFICDDETLEGQA
                     RHYKQRLLEWQEAHHG"
     gene            complement(23198..24529)
                     /locus_tag="J1Y83_001972"
     CDS             complement(23198..24529)
                     /locus_tag="J1Y83_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBA7630121.1"
                     /translation="MQPSRNFDDLKFSSIHRRILLWGSGGPFLDGYVLVMIGVALEQL
                     TPALKLDADWIGLLGAGTLAGLFVGTSLFGYISDKVGRRKMFLIDIIAIGVISVATMF
                     VSSPVELLVMRVLIGIVIGADYPIATSMITEFSSTRQRAFSISFIAAMWYVGATCADL
                     VGYWLYDVEGGWRWMLGSAAIPCLLILIGRFELPESPRWLLRKGRVKECEEMMIKLFG
                     EPVAFEEEQPQQTRFRDLFNRRHFPFVLFVAAIWTCQVIPMFAIYTFGPQIVGLLGLG
                     VGKNAALGNVVISLFFMLGCIPPMLWLNTAGRRPLLIGSFAMMTLALAVLGLIPDMGI
                     WLVVMAFAVYAFFSGGPGNLQWLYPNELFPTDIRASAVGVIMSLSRIGTIVSTWALPI
                     FINNYGISNTMLMGAGISLFGLLISVAFAPETRGMSLAQTSNMTIRGQRMG"
     gene            complement(24588..24875)
                     /gene="fixX"
                     /locus_tag="J1Y83_001973"
     CDS             complement(24588..24875)
                     /gene="fixX"
                     /locus_tag="J1Y83_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like protein FixX"
                     /protein_id="HBA7630122.1"
                     /translation="MTSPVNVDVKLGVNKFNVDEEHPHIVVKADADKQALELLVKACP
                     AGLYKKQDDGSVRFDYAGCLECGTCRILGLGSALEQWEYPRGTFGVEFRYG"
     gene            complement(24872..26158)
                     /locus_tag="J1Y83_001974"
     CDS             complement(24872..26158)
                     /locus_tag="J1Y83_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HBA7630123.1"
                     /translation="MSEDIFDAIIVGAGLAGSVAALVLAREGAQVLVIERGNSAGAKN
                     VTGGRLYAHSLEHIIPGFADSAPVERLITHEKLAFMTEKSAMTMDYCNGDETSPSQRS
                     YSVLRSKFDAWLMEQAEEAGAQLITGIRVDNLVQRDGKVVGVEADGDVIEAKTVILAD
                     GVNSILAEKLGMAKRVKPTDVAVGVKELIELPKSVIEDRFQLQGNQGAACLFAGSPTD
                     GLMGGGFLYTNENTLSLGLVCGLHHLHDAKKSVPQMLEDFKQHPAVAPLIAGGKLVEY
                     SAHVVPEAGINMLPELVGDGVLIAGDAAGMCMNLGFTIRGMDLAIAAGEAAAKTVLSA
                     MKSDDFSKQKLAEYRQHLESGPLRDMRMYQKLPAFLDNPRMFSGYPELAVGVARDLFT
                     IDGSAPELMRKKILRHGKKVGFINLIKDGMKGVTVL"
     gene            complement(26209..27150)
                     /locus_tag="J1Y83_001975"
     CDS             complement(26209..27150)
                     /locus_tag="J1Y83_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HBA7630124.1"
                     /translation="MNTFSQVWVFSDTPSRLPELMNGAQALANQINTFVLNDADGTQA
                     IQLGANHVWKLSGKPDERMIEDYAGVMADTIRQHGADGLVLLPNTRRGKLLAAKLGYR
                     LNAAVSNDASAVSVQDGKATVKHMVYGGLAIGEERIATPYAVLTISSGTFDAAQPDAS
                     RTGETHTVEWQAPAVAITRTATQARQSNSVDLDKARLVVSVGRGIGSKENIALAEQLC
                     KAIGAELACSRPVAENEKWMEHERYVGISNLMLKPELYLAVGISGQIQHMVGANASQT
                     IFAINKDKNAPIFQFADYGIVGDAVKILPALTAALAR"
     gene            complement(27165..27935)
                     /locus_tag="J1Y83_001976"
     CDS             complement(27165..27935)
                     /locus_tag="J1Y83_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein FixA"
                     /protein_id="HBA7630125.1"
                     /translation="MKIITCYKCVPDEQDIAVNNADGSLDFSKADAKISQYDLNAIEA
                     ACQLKQQAAEAQVTALSVGGKALTNAKGRKDVLSRGPDELIVVIDDQFEQALPQQTAS
                     VLAAAAQKAGFDLILCGDGSSDLYAQQVGLLVGEILNIPAVNGVSKIISLTADTLTVE
                     RELEDETETLSIPLPAVVAVSTDINSPQIPSMKAILGAAKKPVQVWSAADIGFNAEAA
                     WSEQQVAAPKQRERQRIVIEGDGEEQIAAFAENLRKVI"
BASE COUNT         6757 a         7803 c         7353 g         6241 t
ORIGIN      
        1 ctcccccttc tttcttatta ttcacatcct gccctaaact cactcggact cgccccggtg
       61 cattttttaa atactcgcga gaaatagagt tgatcgtcaa aaccgacatt gcgaccgacg
      121 gtggcgatag gcatccgggt ggtgctcaaa agcagtttcg cctgactgat gcgctggtcc
      181 tcgcgccagc ttaatacgct aatccctaac tgctggcgga acaaatgcga cagacgcgac
      241 ggcgacaggc agacatgctg tgcgacgctg gcgatatcaa aattactgtc tgccaggtga
      301 tcgctgatgt actgacaagc ctcgcgtacc cgattatcca tcggcggatg aagcgactcg
      361 ttaatcgctt ccatgcgccg cagtaacaat tgctcaagca gatttatcgc cagcaattcc
      421 gaatagcgcc cttccccttg tccggcatta atgatttgcc caaacaggtc gctgaaatgc
      481 ggctggtgcg cttcatccgg gcgaaagaaa ccggtattgg caaatatcga cggccagtta
      541 agccattcat gccagtaggc gcgcggacga aagtaaaccc actggtgata ccattcgtga
      601 gcctccgaat gacgaccgta gtgatgaatc tctccaggcg ggaacagcaa aatatcaccc
      661 ggtcggcaga caaattctcg tccctgattt ttcaccaccc cctgaccgcg aatggtgaga
      721 ttgagaatat aacctttcat tcccagcggt cggtcgataa aaaaatcgag ataaccgttg
      781 gcctcaatcg gcgttaaacc cgccaccaga tgggcgttaa acgagtatcc cggcagcagg
      841 ggatcatttt gcgcttcagc catacttttc atactcccgc cattcagaga agaaaccaat
      901 tgtccatatt gcatcagaca ttgccgtcac tgcgtctttt actggctctt ctcgctaacc
      961 caaccggtaa ccccgcttat taaaagcatt ctgtaacaaa gcgggaccaa agccatgaca
     1021 aaaacgcgta acaaaagtgt ctataatcac ggcagaaaag tccacattga ttatttgcac
     1081 ggcgtcacac tttgctatgc catagcattt ttatccataa gattagcgga tccagcctga
     1141 cgcttttttt cgcaactctc tactgtttct ccatacccgt ttttttggat ggagtgaaac
     1201 gatggcgatt gcaattggcc tcgattttgg cagtgattct gtgcgagctt tggcggtgga
     1261 ctgcgccacc ggtgaagaga tcgccaccag cgtagagtgg tatccccgtt ggcagaacgg
     1321 gcaattttgt gatgccccga ataaccagtt ccgtcatcat ccgcgtgact acattgagtc
     1381 aatggaagcg gcgctgaaaa ctgtgcttgc agagcttagc gtcgaacagc gcgcagctgt
     1441 ggtcgggatt ggcgttgaca caaccggctc gacgcccgca ccgattgacg ccgacggtaa
     1501 cgtgctggcg ctgcgcccgg agtttgccga aaacccgaac gcgatgttcg tattgtggaa
     1561 agaccacacc gcggttgaag aagcggaaga gattacccgc ttatgtcaca cgccgggcaa
     1621 cgttgactac tcccgctata ttggcggtat ttattccagc gaatggttct gggcaaaaat
     1681 cctgcatgta actcgccagg acaacgccgt ggcacaatct gccgcatcgt ggattgagct
     1741 gtgcgactgg gtgccagctc tgctttccgg taccacccgc ccgcaggata ttcgtcgcgg
     1801 acgttgcagc gccgggcata aatctctatg gcatgaaagc tggggtggcc tgccgccagc
     1861 cagtttcttt gatgagctgg acccgatcct caaccgtcat ttaccttccc cgctgttcac
     1921 tgaaacctgg actgccgata ttccggtggg caccttatgc ccggaatggg cgcagcgtct
     1981 cggcctgcct gaaagcgtgg tgatttccgg cggcgcgttt gactgtcata tgggtgcggt
     2041 cggtgcgggc gcacagccta acgcactggt aaaagttatc ggtacttcca cctgcgacat
     2101 tctgattgcc gacaaacaga gcgttggcga gcgggcagtg aaaggtattt gcggtcaggt
     2161 tgatggcagc gtggtgcctg gatttatcgg tctggaagca ggccaatcgg cgtttgggga
     2221 tatctacgcc tggttcggtc gcgtactcgg ctggccgctg gaacagcttg ccgcccagca
     2281 tccggaactg aaagagcaaa tcaacgccag ccagaaacaa ctgcttccgg cgctgaccga
     2341 agcatgggcc aaaaatccgt ctctggatca cctgccggtg gtgctcgact ggtttaacgg
     2401 tcgccgcacg ccaaacgcta accaacgcct gaaaggagtg attaccgatc tgaacctcgc
     2461 caccgacgct ccgctgctgt tcggcggttt aattgccgcc accgcctttg gcgcacgcgc
     2521 aattatggag tgcttcaccg atcaggggat cgccgtcaat aacgtgatgg cgctgggcgg
     2581 catcgcgcgg aaaaaccagg tcattatgca ggcctgctgc gacgtgctga atcgcccgct
     2641 gcaaattgtt gcctctgacc agtgctgtgc gctcggtgcg gcgatttttg ctgctgtcgc
     2701 cgcgaaagtg cacgcagaca tcccatcagc ccagcaaaaa atggccagtg cggtagagaa
     2761 aaccctgcaa ccgcgcagcg aacaggcaca acgctttgaa cagctttatc gccgctatca
     2821 gcaatgggcg ataagcgccg aacaacacta tcttccaact tccgccccgg cacaggctgc
     2881 ccaggccgtt ccgactctat aaggacacga caatgacgat ttttgataat tatgaagtgt
     2941 ggtttgtaat tggcagccag catctttacg gcccggagac tctgcgccag gtgacgcaac
     3001 atgcggaaca cgttgttaat gcactgaata cagaagcgaa gttgccctgc aaactggtgc
     3061 tgaaaccgct gggcaccacg ccggatgaaa tcaccgctat ttgccgcgac gcgaattacg
     3121 acgatcgttg cgctggtctg gtggtgtggc tgcacacctt ctccccggcc aaaatgtgga
     3181 tcaacggcct gaccatgctc aacaaaccgt tgctgcaatt ccacacccag ttcaacgcgg
     3241 cgctgccgtg ggacagcatc gacatggact ttatgaacct gaaccagacc gcgcatggcg
     3301 gtcgcgagtt cggcttcatc ggcgcgcgta tgcgtcaaca acatgccgtc gttaccggtc
     3361 actggcagga taaacaagca catgagcgta tcggctcctg gatgcgtcag gcggtctcta
     3421 aacaggatac ccgtcatctg aaagtctgcc gttttggcga taacatgcgt gaagtagcgg
     3481 tcaccgatgg cgataaagtt gccgcacaga tcaagttcgg tttctccgtc aatacctggg
     3541 cggttggcga tctggtgcag gtggtgaact ccatcagcga cggcgatgtt aacgcgctgg
     3601 tcgatgagta cgaaagctgc tacaccatga cacctgccac acaaatccac ggcgaaaaac
     3661 gacagaacgt gctggaagcg gcgcgtattg agctggggat gaagcgtttc ctggaacaag
     3721 gtggcttcca cgctttcact actacctttg aagatttgca cggcctgaaa cagcttccgg
     3781 gtctggccgt acagcgtctg atgcagcagg gctacggctt tgcgggcgaa ggcgactgga
     3841 aaactgccgc cctgcttcgc atcatgaagg tgatgtcaac cggtctgcag ggcggcacct
     3901 cctttatgga ggactacacc tatcacttcg agaaaggtaa tgacctggta ctcggctccc
     3961 atatgctgga agtctgtccg tcgatcgctg tggaagagaa accgatcctc gacgttcagc
     4021 atctcggtat tggcggtaaa gacgatcctg cccgcctgat cttcaacact caaaccggtc
     4081 cagccattgt cgccagcctg attgatctcg gcgatcgtta ccgtctgctg gttaactgca
     4141 tcgacacggt gaaaacaccg cactccctgc cgaaactgcc ggtggcgaat gcgctgtgga
     4201 aagcgcaacc ggatctacca accgcttccg aagcgtggat ccttgctggt ggcgcgcacc
     4261 ataccgtctt cagccatgcg ctgaacctca acgatatgcg ccagtttgcc gagatgcacg
     4321 acattgaaat cacggtgatt gataacgaca cccgcctgcc agcgtttaaa aacgcgctgc
     4381 gctggaacga agtgtattac gggtttcgtc gctaagtagc cgcatcaggt atgtaacgcc
     4441 tgatgcgacg ctgacgcgtc ttatcaggcc tacgcgttcc ggatttgtag gccggataag
     4501 caaagcgcat ccggcattca ccgcctgatg cgacgccgac gcgtcttatc aggcctacac
     4561 gctgcgattt tgtaggccgg ataagcaaag cgcatccggc acgaaggagt caacatgtta
     4621 gaagatctca aacgccaggt cttagaagcc aacctggcgc tgccaaaaca caacctggtc
     4681 acgctcacct ggggcaatgt cagcgccgtt gatcgcgggc gcggcgtcct ggtgatcaaa
     4741 ccttccggcg tcgactacag catcatgacc gctgacgata tggtcgtggt cagcatcgaa
     4801 accggtgaag tggttgaagg tacgaaaaag ccctcctccg acacgccaac tcaccgactg
     4861 ctctatcagg cattcccgtc tattggcggc attgtgcaca cacactcgcg ccacgccacc
     4921 atctgggcgc aggcgggcca gtcgattcca gcaaccggta ccacccacgc cgactatttc
     4981 tacggcacca ttccctgcac ccgcaaaatg accgacgcag aaatcaacgg tgaatatgag
     5041 tgggaaaccg gtaacgtcat cgtagaaacc ttcgaaaaac agggtatcga tgcagcgcaa
     5101 atgcccggcg tgctggtcca ttctcacggc ccatttgcat ggggaaaaaa cgccgaagat
     5161 gcggtgcata acgccatcgt gctggaagaa gtcgcttata tggggatatt ctgccgtcag
     5221 ttagcgccgc agttaccgga tatgcagcaa acgctgctgg ataaacacta tctgcgtaag
     5281 catggcgcga aggcatatta cgggcagtaa tgactgtata aaaccacagc caatcaaacg
     5341 aaaccaggct ataatcaagc ctggtttttt attggatttt cagcgtggcg caggcaggtt
     5401 ttatcttaac ccgacactgg cgggacaccc cacaagggac cgaagtttcc ttctggctgg
     5461 caacggacaa cgggccgttg caggttacgc ttgcgccgca agagtccgtg gcatttattc
     5521 ccgccgatca ggttccccgc gcccagtata ttttgcaggg tgagcaaggc tttcgcctga
     5581 cgccgctggc gttaaaggat tttcatcgcc agccggtgta tggcctttac tgtcgcgccc
     5641 atcgccagtt gatgaattac gaaaagcgcc tgcgtgaagg tggcgttacc gtctacgagg
     5701 ccgatgtgcg cccgccagaa cgctatctga tggagcggtt tatcacctcg ccagtgtggg
     5761 tcgagggtga tatgcgcaac ggcgctatcg ttaatgcccg tctgaaaccg catcccgact
     5821 atcgtccgcc gctcaagtgg gtttctattg atattgaaac cacccgccac ggtgagctgt
     5881 actgcatcgg cctggaaggt tgcgggcagc gtatcgttta tatgctgggg ccagagaatg
     5941 gcgacgcctc cgcgctcgat tttgaactgg aatacgtcgc cagccgcccg ctgctactgg
     6001 aaaaacttaa cgcctggttt gcgactcatg atcctgatgt gatcatcggt tggaacgtgg
     6061 tgcagttcga tctgcgaatg ctgcaaaaac atgccgagcg ttaccgtatt ccgctgcgtc
     6121 tggggcgtga taacagcgag ctggagtggc gcgagcacgg ctttaaaaac ggcgtctttt
     6181 ttgctcaggc caaagggcga ctgattatcg acggtatcga ggcgctgaaa tccgcgttct
     6241 ggaatttctc ttcattctcg ctggaaaccg tcgctcagga gttattaggc gaaggaaaat
     6301 ctatcgataa cccgtgggat cgaatggacg aaattgaccg ccgtttcgcc gaagataagc
     6361 ctgcgctggc aacttataac ctgaaagatt gcgagctggt gacgcagatc ttccacaaaa
     6421 ctgaaatcat gccgtttttg ctcgaacggg cgacggtgaa cggcctgccg gtagatcgac
     6481 acggcggttc ggtggcagcg tttggtcatc tctattttcc ccgtatgcat cgcgctggtt
     6541 atgtcgcgcc taatctcggc gaagtgccgc cgcacgccag cccaggcggc tacgtgatgg
     6601 attcgcgacc tgggctttat gattcggtgc tggtgctgga ctacaaaagc ctgtacccgt
     6661 cgatcatccg cacctttctg attgatcccg tcgggctggt ggaaggcatg gcgcagcctg
     6721 atccagagca cagtaccgaa ggttttctcg atgcctggtt ctcacgggag aaacattgcc
     6781 tgccggagat tgtgactaac atctggcacg ggcgcgatga agccaaacgt cagggtaata
     6841 agccgctttc gcaggcgctg aagatcatta tgaatgcctt ttatggtgtg ctcggcacca
     6901 ccgcctgccg tttcttcgat ccacgcctgg catcgtcgat caccatgcgt ggtcatcaga
     6961 tcatgcggca aaccaaaacg ttgattgaag cgctgggcta tgacgttatc tacggcgata
     7021 ccgactcaac gtttgtctgg ctgaaaggtg cacattcgga agaagaagca gcgaaaatcg
     7081 gtcgtgcact agtgcagcac gttaacgtct ggtgggcgga aacgctgcaa aaacaacggc
     7141 tgaccagcgc actggaactg gagtatgaaa cccatttctg ccgttttctg atgccaacca
     7201 ttcgcggggc cgataccggc agcaaaaagc gctacgccgg actgattcag gagggcgaca
     7261 aacagcggat ggtatttaaa gggctggaaa ccgtgcgcac cgactggacg ccgctggccc
     7321 agcagtttca gcaggagctg tacctgcgta tcttccgcaa cgagccatat caggaatacg
     7381 tgcgcgaaac catcgacaag ctgatggcgg gcgaactgga cacgcagctg gtttaccgta
     7441 aacgccttcg ccgtccgctg agcgaatatc agcgtaatgt tccgcctcat gtacgcgccg
     7501 ctcgccttgc cgatgaagaa aaccataagc gcggtcgccc cttgcaatat cagaaccgcg
     7561 gcaccattaa gtacgtatgg accaccaacg gcccggagcc gctggactac caacgttcac
     7621 cactggatta cgaacactat ctgacccgcc agctacaacc cgtggcggag ggaatactcc
     7681 cttttattga ggataatttt gctacactta tgaccgggca acttgggcta ttttgagcaa
     7741 aaaaaagagt tcgccagata ccattttgat gcgtgacgaa tgctttgcca tccagtacca
     7801 tagcgccctt tccattcctg gacctgaata acaccactac ctcataagca cggtagcggg
     7861 tggttattgc ctgcaattaa agatatagag ccgaacacat atgcctttta cacttggtca
     7921 acgctggatc agcgatacag aaagcgaatt gggacttgga accgttgtcg cggtggatgc
     7981 gcgaactgtc actttacttt tcccatctac tggtgaaaac cgtctgtacg cacgcagtga
     8041 ttcccccgtg acccgcgtga tgttcaaccc tggtgatacc attaccagcc atgacggctg
     8101 gcagatgcaa gtcgaagaag taaaagaaga aaatggcttg ctgacctata tcggtactcg
     8161 ccttgatact gaagagtccg gcgtggccct gcgtgaagtt ttccttgata gcaaactggt
     8221 gttcagcaaa ccgcaggacc gtctgtttgc cgggcagatt gaccgtatgg accgctttgc
     8281 gctgcgttat cgcgcgcgta agtattccag cgaacagttc cgtatgccgt acagcggcct
     8341 gcgcggtcag cgtaccagcc tgatcccgca ccagctcaac atcgctcatg atgttggtcg
     8401 ccgccacgcg ccgcgcgtcc tgttggctga cgaagtgggt ttagggaaaa ccattgaagc
     8461 cgggatgatc ctgcatcagc aactgctctc tggcgctgct gaacgtgtgc tgattatcgt
     8521 cccggaaacc ttacagcatc agtggctggt agaaatgctg cgccgtttca acctgcgctt
     8581 tgctctgttt gatgatgagc gttatgccga agctcagcac gatgcttaca acccgttcga
     8641 caccgaacag ctggtgattt gctcgctgga ttttgcccgt cgtagcaaac agcgtctgga
     8701 acatctctgt gaagccgagt gggacctgct ggtggtcgat gaagcgcatc acctggtgtg
     8761 gagcgaagat gcgccgagcc gtgaatatca ggccattgaa caactggcag agcacgtgcc
     8821 gggcgttctg ctgctgaccg caaccccgga acagctgggg atggaaagcc acttcgcccg
     8881 tctgcgtctg ctggacccga accgtttcca cgattttgcg caattcgttg aagagcagaa
     8941 aaattatcgt ccggttgcgg acgccgttgc catgctgctg gctggtaaca aactcagcaa
     9001 tgacgaactg aacatgctcg gtgagatgat cggcgagcag gatatcgagc cgctgttgca
     9061 agcagcaaac agcgacagcg aagatgccca gagcgcccgt caggaactgg tttcgatgct
     9121 gatggatcgc cacggtacca gccgcgtgct gttccgtaac acccgtaacg gtgtgaaagg
     9181 cttcccgaaa cgcgagctgc acactattaa gctgccactg ccgacgcagt atcagaccgc
     9241 tattaaagtc tccggcatta tgggcgcacg taaaagtgcg gaagatcgcg ctcgcgatat
     9301 gctctatccg gagcgtattt atcaggaatt tgaaggcgat aacgccacct ggtggaactt
     9361 cgatccgcgc gttgaatggc tgatgggcta cctgaccagc catcgctctc agaaagtgct
     9421 ggtgatctgc gctaaagcgg ccactgcgct gcaactggag caggtactgc gcgaacgtga
     9481 aggtattcgc gccgcagtgt tccacgaagg catgtcgatt atcgaacgtg accgcgctgc
     9541 cgcctggttt gccgaagaag acaccggcgc acaggtactg ctgtgctcgg aaatcggttc
     9601 tgaaggacgt aacttccagt tcgccagcca catggtgatg tttgacctgc cattcaaccc
     9661 ggatctgctg gagcagcgta ttggtcgtct ggatcgtatc ggtcaggcgc acgatattca
     9721 gatccatgtg ccttatctgg aaaaaacggc tcagtcggtg ctggtgcgct ggtatcacga
     9781 aggtctggat gcattcgagc acacctgccc gaccggacgc actatctacg atagcgtgta
     9841 caacgatttg attaactatc tggcctcgcc ggatgagacc gaaggctttg acgatctgat
     9901 caaaaactgc cgcgagcagc atgaagcgct gaaagcacaa ctggaacagg ggcgtgaccg
     9961 cctgctggag atccactcca acggtggcga aaaagcccag gcactggcag aaagcattga
    10021 agagcaggat gacgatacca acctgatcgc cttcgccatg aacctgttcg atattatcgg
    10081 tatcaatcag gacgatcgcg gcgacaacat gatcgtgctg acgccgtccg atcatatgct
    10141 ggtgccggac ttccctggcc tgtcggaaga tggaatcacc atcaccttcg accgtgaagt
    10201 ggcgctggcg cgtgaagatg cgcagtttat tacctgggag catccgctga tccgcaacgg
    10261 tctggatctg atcctttctg gcgataccgg tagcagcacg atttcactgt tgaaaaacaa
    10321 agcgttgccg gtaggtacgc tgttggtgga actgatttat gtggttgaag cccaggctcc
    10381 gaagcagttg cagctcaacc gcttcctgcc accgacgccg gtacgtatgc tgctggataa
    10441 aaacggcaac aacctggcgg cgcaggtaga gtttgaaacc tttaaccgcc agcttaacgc
    10501 ggttaaccgt cacaccggca gcaagctggt taacgcggtg cagcaggatg ttcacgctat
    10561 ccttcagctg ggggaagcgc agatcgagaa atctgcccgt gcattgattg atgcagcgcg
    10621 taacgaagcc gacgaaaaac tgtctgccga gctgtctcgt ctggaagcgc tgcgtgcagt
    10681 taacccgaac attcgtgacg acgaactgac cgccattgag agcaaccgtc agcaggtaat
    10741 ggaaagcctg gatcaggcag gctggcgtct ggatgccctg cgtttgatcg ttgtgacgca
    10801 tcagtaacgg agccgaagat ggggatggaa aactacaatc cgccgcagga cccctggctg
    10861 attgtcctgt atcaggatga ccatattatg gtggtcaaca agccgagcgg tttgttgtct
    10921 gtgccggggc gtctggaaga gcacaaagac agcgtgatga cgcgcattca gcgtgattat
    10981 ccgcaggcag aatcggtgca tcgtctggat atggctacca gcggcgtgat tgtagtggcg
    11041 ctgaccaaag ccgcggagcg ggagttaaaa cgccagttcc gcgagcgcga gccgaaaaaa
    11101 cagtatgtgg cccgcgtctg ggggcatcca tcccctgcgg aaggtctggt ggatctgccg
    11161 ctgatttgcg actggccaaa ccgcccgaaa cagaaagtct gttacgaaac aggtaaacct
    11221 gcgcagacgg agtatgaagt ggtggaatat gcggcggata acacggcaag agtggtgtta
    11281 aaaccgatta ccgggcgttc gcatcagctg cgtgtgcata tgctggcgct gggtcatccg
    11341 attctcggag atcgttttta tgcatcacca gaagcgagag caatggcacc gcgtttgttg
    11401 ctacatgcag agatgctgac gattacccat ccggcgtatg gcaatagtat gacatttaaa
    11461 gcaccagcgg atttttaata gtcacgctgg cgaagttcgg taggccggag aaggcgttca
    11521 cgccgcatcc ggcaattttg cgccgattgc ctgatgcgac gcgggccgcg tcttatcagg
    11581 cctacacgtt gaaacttatt taaacccttt ctgctgcttt atcagctcat aggcctgctg
    11641 aatttcctgc gctttctgct tcgccatctc catcatctct ggcggcaaac ctttcgctac
    11701 caacttatcg ggatggtgtt cactcatcag cttacggtag gcacgtttga tggtggtcgc
    11761 atcatccgtc ggcttcacgc ccagcacatt acaggcatct tccagcgtcg ggccacgctg
    11821 cgcttgctgc cagttaccac cgccagattg ctgctgataa ccgccgccaa actgtgcacc
    11881 gccctgcatc atgcgcaaaa actggtcaaa ctgagcgcgg gagatcccta attcttcagc
    11941 aatgacatac agcaccgccc gttcattcgg gtgcagtgaa ccatcagcaa acgccgcctg
    12001 aatctggatc tccagaaaca tacgaattaa gtcaaaacga ccaaagcaga cactgcgaaa
    12061 ctggcgcatc ttttcgcgca gcgggtaatt gtctgatttt cccacccgga acgcattttg
    12121 cgccgcagta cgggaagcgc catgaaggtt cattcggtcc atcaactggc tggcgatatg
    12181 aatatcagct tccgtgacgc gacctttgga tttggttaaa tgccccatca cttcaaaagt
    12241 ggtggcaaaa aacagcgcct gacgctcacg ctggttggcg aaccacgcca ttttacggct
    12301 acgggcttta tcaaacatat ggccaattaa caggcccaat actacgcccc aaaagccgcc
    12361 gcccattatt aaggccacgg ccacgccaat gatttttccc caatactgca tatattcccc
    12421 aaatcgacac acggatatca gggctatctc ccacaatata aaggcgcttt tacccatttc
    12481 tggctgcggt ctattgtgag acatcgccta taatttgcat tatcatacct gtcattcaat
    12541 gccgtgccta acaccacaga cgctattcgg acaggattaa cactagcgta gagatgacga
    12601 gtacgttagt ctctgaccgt ttgtcacgcg caacgttacc gatgatggaa caataaaatc
    12661 aacgtatgaa aaaacgtatc cccactctcc tggccaccat gattgccact gccctttata
    12721 gtcaacaggg actggcagcc gacctcgcct cacagtgcat gttgggcgtg ccaagctatg
    12781 accgtcctct ggtacagggc gataccaatg acttacccgt gactatcaat gctgaccacg
    12841 cgaaagggga ctacccggat gacgccgtgt ttactggcag cgtggatatc atgcagggta
    12901 acagccgtct gcaggccgac gaagtgcagc tccatcaaaa agaggcacca ggacaaccgg
    12961 agccggtacg taccgttgat gcgctcggta atgtccatta cgacgataac caggtgatcc
    13021 tcaaagggcc gaaaggctgg gcgaatctga acaccaaaga taccaacgtc tgggagggtg
    13081 attaccagat ggtgggtcgc cagggtcgcg gtaaagcgga cctgatgaaa caacgtggcg
    13141 aaaaccgcta taccattctg gataacggta gctttacctc ctgtctgccg ggttctgaca
    13201 cctggagcgt ggtaggtagc gaaattattc atgaccgcga agaacaagtt gcggagatct
    13261 ggaacgcccg ctttaaggtg ggtccggtac cgatctttta tagcccgtat ttgcagttgc
    13321 cggtgggtga caaacgtcgc tctggtttct tgatcccgaa cgccaagtac accaccacca
    13381 actactttga gttctacctg ccatattact ggaacatcgc gccaaatatg gatgccacca
    13441 tcacgccgca ttatatgcat cgtcgtggca acatcatgtg ggagaacgaa ttccgctacc
    13501 tctcccaggc gggcgctggc ttgatggaac tggactatct gccttcagat aaagtctata
    13561 aagatgaaca cccgaacgat gacagttcac gtcgttggtt gttctactgg aaccactccg
    13621 gggtcatgga tcaggtgtgg cgtttcaacg tcgactacac caaagtcagc gatcctagct
    13681 acttcaatga cttcgataac aaatacggtt ccagtaccga cggctacgca acgcaaaaat
    13741 tcagcattgg ctatgcggtg cagaacttca atgccaccgt ttcaaccaag cagttccagg
    13801 tcttcagcga gcagaacacc agtagctact cggcagaacc gcagttagac gttaactact
    13861 atcaaaacga tgtcggtcct ttcgatacgc gaatttacgg ccaggcggtg cactttgtta
    13921 acaccagaga tgacatgcca gaggcgactc gtgttcacct ggaaccgacc atcaatctgc
    13981 cgctctctaa taactggggc agcatcaata ctgaagcgaa gttgctggca acccattatc
    14041 agcaaaccaa tcttgactgg tataactcca gaaacacgac caggctggca gagtccgcta
    14101 accgtgtaat gccgcagttc aaggtagacg gtagaatggt cttcgagcgt gatatggaaa
    14161 tgctggcgcc gggctatacc cagacactgg aaccacgcgc gcagtatctg tatgtgccat
    14221 atcgcgatca gagcaaaatc tataactacg actcgtcgct gctgcaatct gactactccg
    14281 gcctgttccg ggaccggact tacggcggtc ttgaccgtat tgcctccgct aaccaggtga
    14341 cgaccggtgt cacatctcgc atatatgatg atgctgccgt tgaacgtttt aatatttccg
    14401 ttggtcaaat ctactatttc acggagtctc gcactggcga tgacaacata acatgggaga
    14461 atgacgacaa aacgggctca ctggtatggg caggcgatac ttactggcgc atctctgatc
    14521 gttggggatt gcgtggcggg attcagtacg atacacgtct ggataacgta gcgaccagta
    14581 actccagcat tgaataccgt cgggatgaag accgtctggt acagctgaat taccgttacg
    14641 ccagcccgga atatattcag gcaacgctgc ctaagtacta ttccactgct gagcaatata
    14701 agaatggtat ttcgcaggta ggtgccgtcg ccagctggcc aattgccgat cgttggtcca
    14761 ttgttggggc ctactactac gacaccaatg cgaacaagca agccgactct atgttaggtg
    14821 tgcaatacag ctcctgctgc tatgcgattc gcgtcggtta cgagcggaag ctgaacggtt
    14881 gggataacga taaacaacat gcggtatatg acaacgcaat cggctttaac atcgaacttc
    14941 gcggcctgag ctccaactac ggtctgggta cgcaagagat gctgcgttcg aacattctgc
    15001 cgtatcaaaa cactttgtga tttgttgatt taccacgtaa tccgcagtgc ggttaattga
    15061 aatggaaaaa gtatgaagaa ctggaaaacg ctgcttctcg gtatcgccat gatcgcgaat
    15121 accagtttcg ctgcccccca ggtagtcgat aaagtcgcag ccgtcgtcaa taacggcgtc
    15181 gtgctggaaa gcgacgttga tggattaatg cagtcggtaa aactgaacgc tgctcaggca
    15241 aggcaacaac ttcctgatga cgcgacgctg cgccaccaaa tcatggagcg tttgatcatg
    15301 gatcaaatca tcctgcagat ggggcagaaa atgggagtga aaatctccga tgagcagctg
    15361 gatcaggcga ttgctaacat cgcgaaacag aacaacatga cgctggatca gatgcgcagc
    15421 cgtctggctt acgatggtct gaactacaac acctatcgta accagatccg caaagagatg
    15481 attatctctg aagtgcgtaa caacgaggtg cgtcgtcgca tcaccatcct gccgcaggaa
    15541 gtcgaatccc tggcgcagca agtgggtaac caaaacgacg ccagcactga gctgaacctg
    15601 agccacatcc tgatcccgct gccggaaaac ccgacctctg atcaggtgaa cgaagcggaa
    15661 agccaggcgc gcgcgattgt cgatcaggcg cgtaacggcg ctgatttcgg taagctggcg
    15721 attgctcatt ctgccgacca gcaggcgctg aacggcggac agatgggctg gggccgtatt
    15781 caggagttgc cggggatctt cgcccaggca ttaagcaccg cgaagaaagg cgacattgtt
    15841 ggcccgattc gttccggcgt tggcttccat attctgaaag ttaacgacct gcgcggcgaa
    15901 agcaaaaata tctcggtgac cgaagttcat gctcgccata ttctgctgaa accgtcgccg
    15961 atcatgactg acgaacaggc ccgcgtgaaa ctggaacaga ttgctgccga tatcaagagt
    16021 ggtaaaacga cttttgctgc cgcagcgaaa gagttctctc aggatccagg ctctgctaac
    16081 cagggcggtg atctcggctg ggctacacca gatattttcg atccggcctt ccgtgacgcc
    16141 ctgacccgcc tgaacaaagg tcaaatgagt gcaccggttc actcttcatt cggctggcat
    16201 ttaatcgaac tgctggatac ccgtaatgtc gataaaaccg acgcggcaca gaaagatcgt
    16261 gcataccgca tgctgatgaa ccgtaagttc tcggaagaag cagcaagctg gatgcaggaa
    16321 caacgtgcca gcgcctacgt taaaatcctg agcaactaat ggttaaaacc caacgtgttg
    16381 tgatcactcc cggcgagccc gccgggattg gcccggactt aattgtccag cttgcacagc
    16441 gtgagtggcc agtagaactg gttgtttgtg ccgatgccac tctccttacc gaccgggcag
    16501 cgatgctcgg tttgccgctc accctccgcc cttattcccc caactcccct gcacaaccgc
    16561 aaactgcggg cacattaacg ctacttcctg tcgcgctacg tgaatctgtc actgtggggc
    16621 agttagcagt tgaaaatggg cactatgtgg tggaaacgct ggcgcgagcg tgtgatggct
    16681 gtctgaacgg tgaatttgct gcgctgatca caggccccgt gcataaaggc gtcattaacg
    16741 acgcaggcat tccgtttacc gggcataccg agtttttcga agagcgttcg caggcgaaaa
    16801 aagtggtgat gatgctggcg accgaagaac ttcgcgtggc gctggcaacg acgcatttac
    16861 cgctgcgcga tatcgcagat gctatcaccc ctgccctttt gcacgaagtg attgctattt
    16921 tgcatcacga tttgcggacc aaatttggta ttgccgaacc gcgcattctg gtctgcgggc
    16981 tgaatccgca cgcgggcgaa ggcggtcata tgggtacgga agagatagac accattattc
    17041 cggtactcaa cgagctgcgg gaacagggga tgaaactcaa cgggccgctg cctgccgata
    17101 ccctgtttca gccgaaatat ctcgataacg ccgacgccgt gctggcgatg taccacgatc
    17161 agggtcttcc cgtgctaaaa taccagggct tcgggcgcgg tgtgaacatt acgctgggcc
    17221 tgccctttat tcgcacatca gtggaccacg gcaccgcgct tgaactggcg ggacgtggta
    17281 aagccgatgt cggcagtttt attacggcgc ttaatctcgc catcaaaatg attgttaaca
    17341 cccaatgaat aatcgagtcc accagggcca cttagcccgt aaacgcttcg ggcaaaactt
    17401 tctcaacgat cagttcgtga tcgacagcat tgtttctgcc attaacccgc agaagggcca
    17461 ggcgatggtc gaaatcggcc ccggtctggc ggcattgacc gaaccggtcg gcgaacgtct
    17521 ggaccagctg acggtcatcg aacttgaccg cgatctggcg gcacgtctgc aaacgcatcc
    17581 attcttaggc ccgaaactga cgatttatca gcaggatgcg atgaccttta actttggcga
    17641 actggccgcg aaaatgggcc agccgctgcg tgttttcggc aacctgcctt ataacatctc
    17701 cacgccgttg atgttccatc tgtttagcta tactgatgcc attgccgaca tgcactttat
    17761 gttgcaaaaa gaagtggtga atcgtctggt tgcaggaccg aacagcaagg cgtatggtcg
    17821 attaagcgtc atggcgcaat actattgcaa cgtgatcccg gtgctggaag taccgccgtc
    17881 agcctttaca ccaccaccca aagtggattc cgccgtcgtg cgcctggttc ctcatgcaac
    17941 gatgcctcac ccggttaaag atgttcgcgt gttgagccgc atcaccactg aagcctttaa
    18001 ccagcgtcgt aaaaccattc gtaacagcct cggcaacctg tttagcgtcg aggtgttaac
    18061 gggaatgggg atcgacccgg cgatgcgagc ggaaaatatc tctgtcgcgc aatattgcca
    18121 gatggcgaac tatctggcgg agaacgcgcc tttgcaggag agttaacgat gatcaattcg
    18181 ccccgagtgt gtattcaggt tcaaagcgtc tacattgagg cccaatcttc acctgataat
    18241 gaacgttacg tttttgctta taccgtaacc atacgcaatc tggggcgagc gccagtgcag
    18301 ttgttggggc gttactggct gatcaccaat ggcaatggcc gtgaaaccga agtccagggc
    18361 gaaggagtgg ttggcgtcca gccacttatc gcgcctggcg aagagtacca gtacaccagc
    18421 ggtgcaatca ttgaaacccc gctgggcacc atgcagggtc actacgaaat gatcgatgaa
    18481 aatggcgtcc ctttcagcat cgacattccc gtattccgac tcgccgttcc cacactcatt
    18541 cattaaaaga atatggcgac ataccttatt ggcgacgttc atggttgtta cgatgaactg
    18601 atcgcattgc tgcataaagt agaatttacc cctgggaaag ataccctctg gctgacgggc
    18661 gatctggtcg cgcgcggccc gggttcgctg gatgttctgc gctatgtgaa atccttaggc
    18721 gacagcgtac gtctggtgct gggtaatcac gatctgcatc tgctggcggt atttgccggg
    18781 atcagccgca ataaaccgaa agatcgcctg acaccgctgc tggaagcgcc ggatgccgac
    18841 gagctgctta actggctgcg tcgccagcct ttgctgcaaa tcgacgaaga gaaaaagttg
    18901 gtgatggccc acgccgggat cacgccgcag tgggatctgc agaccgccaa agagtgcgcg
    18961 cgcgatgtag aagcggtgct gtcgagtgac tcctatccct tcttccttga tgccatgtac
    19021 ggcgatatgc caaataactg gtcaccggaa ttgcgggggc tgggaagact gcgttttatc
    19081 accaacgcct ttacccgtat gcgtttttgc ttcccgaacg gtcaactgga tatgtacagc
    19141 aaagaatcgc cggaagaggc ccctgcccca ctgaaaccgt ggtttgcgat tcctggcccc
    19201 gtcgctgaag agtacaacat cgcctttggt cactgggcat cgctggaagg caaaggtacg
    19261 ccggaaggta tttacgcgct ggatactggc tgctgctggg gtggtacatt aacctgcctg
    19321 cgctgggaag ataaacagta ttttgtccag ccgtcgaacc ggcataaaga tatgggtgag
    19381 ggagaggcgg ccgcgtctta aatcccgtcg accataaaat ctaatgctcc tttaatcaac
    19441 gttcggtgcg cgctggcatc gcaaaatttc gttcccagcg cgttaactgg aacagttgca
    19501 gtctcatgcg tcatgagcac atattctttg ccatctatca gtaaaaggat tggattaagc
    19561 cgttctggtg ggcgaatacg gctaaatcgt tcaataggtg ttaatggaat aacgatacgg
    19621 cgattaatct caccaataat gtcgctggta acatcaataa caaagggaaa cgcggcgttc
    19681 ctgccgcgac tgcggtatac cgtaaattgc atcgctagaa gctccggtac tcatcagaga
    19741 aacatccggt ttcatcagcc aattgattta acgcagcgat agcttctgcg ttctcttcac
    19801 gccaacgtgt tgctgcatgt tttttaagtt cagcattgag cgcaaccgta agggtggcgc
    19861 tcatatttaa gcctgcatca cgagcgcgat tgactaactc acggtcgacg gtgacggtca
    19921 cactttgtgt ggtacgtttt gcagtcatga tctcgtgctc ctgtacaatt tatacgtcta
    19981 gtatacacgg atatctgcac gtatcagtga tgccggaatt ctaatataca aaattaccgc
    20041 cgctccagaa tctcaaagca atagctatga gagttctgcg catcggcgtc gtggaattca
    20101 ctgaataccg attcccagtc atccggctcg taatccggga aatgggtgtc gccttccact
    20161 tccgcgtcga tatgcgtcag atacagtttt tgcgctttcg gcaggaactg ctcataaacg
    20221 cgaccgccgc caatcaccat gatttctggt acgtcaccac acgccgcaat ggcttcatcc
    20281 accgacttca cccacgttac gcgatcgtcc gtacccggtt gactgctgag gataatattt
    20341 ttgcgtcctg gcaacggacg accgatggat tcccaggtat ggcggcccat aatcacgggt
    20401 ttatttaaag tgttgcgttt aaaccaggcg agatcggcag gcaggttcca cggcatggcg
    20461 ttttccatgc cgataacgcg atctaccgct aacgccgcaa tcagactgat cattgagatt
    20521 tcccgataaa aaaaattgtc gccactatac gtaaagcgta aaccgtcgtc gactggtgcg
    20581 aggatgatgt tgaggaaaat tttatattct gctggcgagt ccacgctctc tccctggact
    20641 cgccgcatta caatgaaaca aaaacaaaca gttagctgta aagcgtgatt tacgtcactc
    20701 tttattagga tgagggcttc gtttccggtt catccgccat attgccagta tgtttccctt
    20761 cttcggttcc ctgccagcca tgtcgttgaa ttaatgacag atgttcacgg tcttcggtaa
    20821 tgatctcact taacatcgcg ctggtgcgtt tatagaccgc cgcacgggct ttggtgtcgt
    20881 tctcaaccat tgccatctct tccaccatct gaatattaaa gcggcggaac acatcggcac
    20941 gttctcgcgc ttcatacggc cccagaccta aactttccag tgccagacgc ccggttttca
    21001 gcgcaccttc gaaggtttca cgctccggct tttcaacgcc tgcctgacgc aaacgaatgt
    21061 agtggtcgac atcgcgggcg cgggcaataa tctgcaaatg tgggaaatgt tctttcacca
    21121 tctccgtcag ttgcaggttg gtttgcggat cgtcgatggc gttaatcagc acttccgctt
    21181 tcgccgctcc ggcagattcc agtaaatcca tccgcgtggc atcgccataa aacactttca
    21241 taccgaattt acgcagcgtt tcgatatggt ccgggtcgtg atcgagcacc accattttca
    21301 ccccgctgga gagcagtaaa cgtccggtta tctgcccaaa acgaccgaat ccggcaatga
    21361 ttacccgcgg ctgttcttcg tcgatctcat cggcttcacg cgcctcttca gtagaagatt
    21421 gctcaaggcg attgaggatc accagcagaa tcggcgttgc cgccatcgac agtgccaccg
    21481 ccagggtcag cgatttcgcc cactctggct ccagcacatt cgccatctgc gccgcaccaa
    21541 atactacaaa ggcaaactca ctaccctgcc ctaatagcac cgcaaaccag cgacgctgtt
    21601 tatttggcac ctgcaacggc cgggcaatca gccacagcat ggcgattttg atgatgagga
    21661 aaccgagcag caaaatgaca atgcgcaacg gattttcaag cagcgtgcca aagtctatcg
    21721 acatgccaac gccgatgaaa aacagcccca gcagcaaacc tttaaatggt tcgatatcgc
    21781 tttccagcgc atgacggtat tccgagcttg ccagcagtac gcccgccaga aacgcgccca
    21841 tcgccatcga caagccgacc tcttccagca gcaaaccaaa gccaaacacg aggaataacg
    21901 ccacggcact aaacacttcc cgcaagccag agcgggcaac aaaacgcagt gccggacgcg
    21961 tgacatagcg ccccagcaat accaccagca ccagcgcgcc cgccactttt aacgccgaga
    22021 gaacaaatgc gcccatcgtc gtcgaggcac tgctcgctgc cagtagcggg atcatcgcca
    22081 ccagtgggat cgccgcgata tcctggaaca gcagcaccgc aaaggcactg cggcccattt
    22141 gcgtcaccat cagattgcgt tcattcatcg cctgcatggc aatcgccgta gaggagagcg
    22201 ccagcgtcat gccgatcaat tccgcgacct gccagcgcag cccaagtaac atgcagaaca
    22261 gccccagcag gccgccgcaa atcaccatct gcaatgcgcc accgccgaac actgccgcac
    22321 gcagcttcca cagcctttgt ggatcgagtt cgaggccgat aataaacagc atcagcacca
    22381 cgccaatctc tgcaaagtgc agaatagatt cggcatcggt caccagtcgc agcccccacg
    22441 ggccaataat gcagccggcg atcaggtagc caagaaccga tcccagaccg agacgtaccg
    22501 caatgggaac aatcagcgcc gccgaaccga gataaatcag cgcctgaatc agcgtatggc
    22561 tatccatgat gtgcctcctg ccattccagc agacgttgct tatagtgccg cgcctgccct
    22621 tcgagggttt cgtcgtcaca aataaaggtg cagtgcatgg caaacggtgg cagccagttc
    22681 agcccgcagt agattgccgt cgcctgtagc ggctgcgaca gcacatcaaa gcccggatgc
    22741 gcaccaatct caaaatggct ttccccgccg ccggtcgtca ccgcccacag caaatgtttg
    22801 ccgtgcagcg ccgtgccgcc gtgaccgtaa gcccatccgt gcgagaaaac tttatcgatc
    22861 caaagtttga ggagcggagg aatgctgtac cactgcatcg ggtgctgcca gacgatcaga
    22921 tcggcacgag acagcgcctc ctgttcggcg gcaatgtcga tattgaagtc aggatagagt
    22981 tgataaagag agcgaatttc gacgccttcc agcgtccttg cctgttcaag catccgttta
    23041 ttcgcatggg aatgatgcgg atatggatgc gcataaatta taagaatcat aggtagcccg
    23101 cgtgattacg cctttgtttt cccacaaagt gtagtcatat attcacgcag cttatagtca
    23161 ataatattga ttcagaaaag agagaaatct gaacaaatta ccccattctc tgcccgcgga
    23221 tcgtcatatt gctggtctgc gccagtgaca tccctcgagt ctccggggca aacgctacgg
    23281 aaatcaacaa gccaaacagc gagatacccg cccccattag catcgtgtta ctgataccgt
    23341 aattattgat aaagatcggc agtgcccagg tcgatacaat agtgccaata cggcttaagg
    23401 acataatcac gcccacggca gaggcgcgga tatccgtcgg gaagagttca ttaggataga
    23461 gccactgcaa attacccggc ccaccagaga aaaaggcata caccgcaaag gccatcacta
    23521 ccagccagat ccccatatcc gggatcagcc ccaaaaccgc cagcgccagc gtcatcatgg
    23581 caaagctgcc aatcaacaat ggacgccgtc cggcagtgtt tagccacagc atcggcggaa
    23641 tacagccgag cataaagaac agactaatca ccacgttccc cagcgccgcg tttttgccaa
    23701 cccccaatcc taacaaacca acgatttgcg ggccaaaggt gtaaatggcg aacattggga
    23761 tcacctggca ggtccagatg gcggcaacaa acagaacaaa aggaaaatgg cggcgattaa
    23821 acagatcgcg aaaacgggtt tgctgcggct gctcttcctc gaaagccacc ggttcgccaa
    23881 acagttttat catcatctct tcgcactctt ttactcgccc tttgcgtaac aaccagcggg
    23941 gagattcagg cagttcgaat cgaccaatca aaatcaataa acagggaatc gctgcgctac
    24001 ccagcatcca gcgccagccg ccttccacat cataaagcca atagccgacc aaatcggcgc
    24061 aggtcgcgcc gacataccac atcgcggcga taaagctgat ggaaaacgcc cgctgacggg
    24121 tactggagaa ctcggtgatc attgaggtgg cgatgggata atccgcaccg ataacaatgc
    24181 cgataagtac ccgcatcacc aacagttcga cgggggatga gacaaacatc gtcgccaccg
    24241 atatcacgcc gatggcgatg atatcaatga ggaacatttt gcgccgtccg actttatcgg
    24301 agatatagcc aaacagcgat gtgccaacga acagcccggc gagcgttccc gcgcccagca
    24361 agccaatcca gtcagcgtcc agtttcagcg ccggagtcag ttgctccagc gccacgccaa
    24421 tcattaccag tacataacca tccagaaacg gaccaccgct tccccacagc aaaatgcggc
    24481 ggtgaataga ggagaattta agatcatcaa agtttctgga cggttgcatg ggttattcct
    24541 gtttttttgc aatgagagtc agtacggcat tacgtatcaa accaacatca gccgtaacgg
    24601 aactccacac caaaggtgcc gcgcgggtat tcccactgtt ccagcgccga ccccagcccc
    24661 agaatgcgac aggtgccgca ctccagacat ccggcgtaat cgaagcgcac actgccgtca
    24721 tcctgcttct tgtacagacc tgcggggcac gctttcacca gcagctccag ggcctgttta
    24781 tcagcatcgg ccttcacaac gatgtgcgga tgctcttcat cgacattgaa tttattgacg
    24841 cccagtttga cgtccacatt gacgggagaa gtcataaaac ggtcactcct ttcatgccat
    24901 ccttgatcag attgatgaag cccactttct tgccgtggcg gaggattttc ttgcgcatca
    24961 gttccggcgc actgccgtca atggtgaaca ggtcacgcgc cacgcccacc gccagttccg
    25021 ggtagccgct aaacatgcgt gggttatcaa ggaacgccgg tagtttctgg tacatgcgca
    25081 tatcgcgcag cgggccactc tcaagatgct gacgatattc cgcgagtttt tgcttactga
    25141 aatcgtcgct tttcatcgct gaaagcacag tttttgctgc tgcttccccg gcggcaatcg
    25201 ccagatccat accgcgaatg gtaaaaccga ggttcataca cattccggcg gcatcaccgg
    25261 caatcaatac gccgtcacca accaactccg gcagcatgtt gatacctgct tccggcacta
    25321 cgtgagcgga atattccacc agcttgccgc ccgcgatcag cggtgcaacg gccggatgtt
    25381 gtttgaaatc ttccagcatt tgcggcaccg atttttttgc gtcatgcaga tgatgcaaac
    25441 cacaaaccag ccccagcgac agggtatttt cattggtata aaggaagcca ccgcccatca
    25501 ggccatcggt gggtgatccc gcaaacaggc aagccgcgcc ctgattaccc tgcaactgaa
    25561 aacggtcttc gataaccgac ttcggtaact cgatcagttc ctttacgcca accgccacat
    25621 ccgtcggttt aacgcgtttt gccatcccca gcttttcggc aaggatggaa ttcaccccat
    25681 cagcaaggat caccgttttc gcttcaatca catcgccatc ggcttctaca ccgacgactt
    25741 tgccatcgcg ctgtacgagg ttatcgacgc ggatcccggt aattaactgc gcgcccgctt
    25801 cttcagcctg ctccatcagc caggcatcaa atttactgcg caaaacggag taagaacgct
    25861 gggatggcga ggtttcgtcg ccattgcagt agtccatagt catcgctgac ttttccgtca
    25921 taaacgcgag tttttcatgg gtgatcaggc gttctacggg ggcggagtca gcgaaaccag
    25981 gaataatgtg ttccagactg tgggcataga gacgcccgcc ggtgacgttc ttggcacctg
    26041 cggaattgcc tcgctcgata actaacactt gcgcaccttc gcgggcgagc actagtgcgg
    26101 caaccgaacc ggcaagccct gcaccgacga tgatggcgtc aaagatatct tcggacataa
    26161 gagctccctg tcagcggcag ggccaaaatg cagccctgcc agagtggatc aacgcgctaa
    26221 agctgcggtc agtgccggaa ggatctttac ggcgtcgcca acaatgccgt aatccgcgaa
    26281 ctggaagatc ggcgcatttt tatctttgtt gatggcgaaa atggtttgcg acgcattagc
    26341 gccaaccatg tgctggatct gcccggagat ccccaccgcc aggtacagtt caggtttcag
    26401 catcaggttg gagataccga catagcgttc gtgctccatc catttttcgt tttccgccac
    26461 cggacgagaa caggccaact ccgcacctat cgccttgcaa agctgttctg ccagcgcaat
    26521 gttctctttg ctgccaatac cgcgaccaac gctgaccacc aggcgggctt tgtcgagatc
    26581 gacgctgttg ctctgacgcg cctgggttgc cgtgcgggta atcgccacag ccggagcctg
    26641 ccactccacg gtgtgcgttt cgccagtgcg tgacgcgtct ggctgagccg catcgaacgt
    26701 gccgctgctg atggtcagta ccgcatacgg cgtggcaatg cgttcttcgc caatcgccag
    26761 accgccgtaa accatgtgtt tcactgtcgc tttaccgtcc tgcacgctaa cagcgctggc
    26821 atcgttagac accgccgcgt taaggcggta acccagtttc gccgccagta atttgccgcg
    26881 acgggtgttt ggcaacagca ccagaccgtc tgcgccgtgc tggcgaatag tgtcagccat
    26941 gacaccggcg taatcttcga tcatccgctc gtccggtttg ccgcttaatt tccagacatg
    27001 attagcgccg agctggattg cctgtgtgcc gtcggcatca ttgaggacga acgtgttgat
    27061 ttgattagct aaagcctgcg caccgttcat cagttccggc agacgagaag gggtatcgct
    27121 gaatacccag acttgagaaa acgtgttcat agcatcccct gtaattaaat gactttgcga
    27181 agattttcag caaatgcggc gatctgttct tcgccgtcgc cttcaatcac gatgcgctga
    27241 cgctcgcgct gtttcggcgc ggcaacctgt tgttctgacc aggctgcctc tgcgttaaaa
    27301 ccgatatccg ccgccgacca tacctggact ggctttttcg ccgcgccgag aatggctttc
    27361 atcgaaggaa tttgtgggga gttgatatca gtggaaacag caacaaccgc aggcagcgga
    27421 atgcttaacg tttctgtttc atcttccagt tcgcgctcaa cggtgagggt atctgcagtc
    27481 agggagataa ttttgctgac gccgttaact gccggaatat taaggatttc gcctaccagc
    27541 aaaccaacct gctgggcata aaggtcggaa gaaccatcgc cacagaggat caaatcaaag
    27601 cctgctttct gagcggctgc agcaagtacg ctcgccgttt gttgcggcag tgcctgctcg
    27661 aactggtcat caatcaccac aatcagttca tccggaccgc gcgatagcac atctttacgc
    27721 cctttggcgt tggtcagggc tttaccgccc acacttaagg ccgtcacctg cgcctctgct
    27781 gcctgttgct ttagctggca agccgcttca atagcgttga gatcgtattg gcttattttg
    27841 gcatcggctt tgctgaagtc taacgaacca tcagcattat tgaccgcaat atcctgttca
    27901 tcaggcacgc acttatagca agtaataatc ttcattgcat ctccagaaat catgaaggaa
    27961 acgtcgttca tatcggtggt gttaattaca gatatcacgc cctttaattt ggaaataaaa
    28021 ctttaatcac caatattgaa aatgtcacac gcagataaat ccaactttca atattgttaa
    28081 gctcctcacc aatattgaaa acatggcgta gaaaaaagaa attttcaata ttgttttatg
    28141 gatcaccaat attg
//
LOCUS       DADSYM010000055        28076 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2415
            SAMN08039579-rid11723173.denovo.055, whole genome shotgun sequence.
ACCESSION   DADSYM010000055 DADSYM010000000
VERSION     DADSYM010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039579
            Sequence Read Archive: SRR6362533
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 28076)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28076)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980162.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 03:39:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,484
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 4,347
            CDSs (with protein)               :: 4,347
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 43 of 85
            Pseudo Genes (incomplete)         :: 37 of 85
            Pseudo Genes (internal stop)      :: 19 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28076
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039579-rid11723173.denovo.055"
                     /strain="LGE2415"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8302 N 95.9628 W"
                     /collection_date="2015-08-24"
     gene            complement(12..1466)
                     /gene="xylB"
                     /locus_tag="J1Y83_001977"
     CDS             complement(12..1466)
                     /gene="xylB"
                     /locus_tag="J1Y83_001977"
                     /EC_number="2.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylulokinase"
                     /protein_id="HBA7630126.1"
                     /translation="MYIGIDLGTSGVKVILLNEQGEVVAAQTEKLTVSRPHPLWSEQD
                     PEQWWQATDRAMKALGVQHSLQDVKALGIAGQMHGATLLDAQQRVLRPAILWNDGRCA
                     QECTLLEARVPQSRVITGNLMMPGFTAPKLLWVQRHEPEIFRQIDKVLLPKDYLRLRM
                     TGEFASDMSDAAGTMWLDVAKRDWSDLMLQACHLSRDQMPALYEGSEITGALLPEVAK
                     AWGMAAVPVIAGGGDNAAGAVGVGMVDANQAMLSLGTSGVYFAVSEGFLSKPESAVHS
                     FCHALPQRWHLMSVMLSAASCLDWAAKLTGLSNVPALITAARQADESAEPVWFLPYLS
                     GERTPHNNPQAKGVFFGLTHQHGPNELARAVLEGVGYALADGMDVVHACGIKPQSVTL
                     IGGGARSEYWRQMLADISGQQLDYRTGGDVGPALGAARLAQIAANPEKSLIELLPQLP
                     LEQSHLPDAQRYAAYQPRRETFRRLYQQLLPLMA"
     gene            complement(1538..2860)
                     /gene="xylA"
                     /locus_tag="J1Y83_001978"
     CDS             complement(1538..2860)
                     /gene="xylA"
                     /locus_tag="J1Y83_001978"
                     /EC_number="5.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylose isomerase"
                     /protein_id="HBA7630127.1"
                     /translation="MQAYFDQLDRVRYEGSKSSNPLAFRHYNPDELVLGKRMEEHLRF
                     AACYWHTFCWNGADMFGVGAFNRPWQQPGEALALAKRKADVAFEFFHKLHVPFYCFHD
                     VDVSPEGASLKEYINNFAQMVDVLAGKQEESGVKLLWGTANCFTNPRYGAGAATNPDP
                     EVFSWAATQVVTAMEATHKLGGENYVLWGGREGYETLLNTDLRQEREQLGRFMQMVVE
                     HKHKIGFQGTLLIEPKPQEPTKHQYDYDAATVYGFLKQFGLEKEIKLNIEANHATLAG
                     HSFHHEIATAIALGLFGSVDANRGDAQLGWDTDQFPNSVEENALVMYEILKAGGFTTG
                     GLNFDAKVRRQSTDKYDLFYGHIGAMDTMALALKIAARMIEDGELDKRIAQRYSGWNS
                     ELGQQILKGQMSLADLAKYAQEHNLSPVHQSGRQEQLENLVNHYLFDK"
     gene            3226..4218
                     /gene="xylF"
                     /locus_tag="J1Y83_001979"
     CDS             3226..4218
                     /gene="xylF"
                     /locus_tag="J1Y83_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose ABC transporter substrate-binding
                     protein"
                     /protein_id="HBA7630128.1"
                     /translation="MKIKNILLTLCTSLLLTNVAAHAKEVKIGMAIDDLRLERWQKDR
                     DIFVKKAESLGAKVFVQSANGNEETQMSQIENMINRGVDVLVIIPYNGQVLSNVVKEA
                     KQEGIKVLAYDRMINDADIDFYISFDNEKVGELQAKALVDIVPQGNYFLMGGSPVDNN
                     AKLFRAGQMKVLKPYVDSGKIKVVGDQWVDGWLPENALKIMENALTANNNKIDAVVAS
                     NDATAGGAIQALSAQGLSGKVAISGQDADLAGIKRIAAGTQTMTVYKPITLLANTAAE
                     IAVELGNGQEPKADTTLNNGLKDVPSRLLTPIDVNKNNIKDTVVKDGFHKESEL"
     gene            4296..5837
                     /gene="xylG"
                     /locus_tag="J1Y83_001980"
     CDS             4296..5837
                     /gene="xylG"
                     /locus_tag="J1Y83_001980"
                     /EC_number="7.5.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose ABC transporter ATP-binding protein"
                     /protein_id="HBA7630129.1"
                     /translation="MPYLLEMKNITKTFGSVKAIDNVSLRLNAGEIVSLCGENGSGKS
                     TLMKVLCGIYPHGSYEGEIIFAGEEIQASHIRDTERKGIAIIHQELALVKELTVLENI
                     FLGNEITHNGIMDYDLMTLRCQKLLAQVSLSISPDTRVGDLGLGQQQLVEIAKALNKQ
                     VRLLILDEPTASLTEQETSVLLDIIRDLQQHGIACIYISHKLNEVKAISDTICVIRDG
                     QHIGTRDAAGMSEDDIITMMVGRELTALYPNEPHTTGDEILRIEHLTAWHPVNRHIKR
                     VNDVSFSLKRGEILGIAGLVGAGRTETIQCLFGVWPGQWEGKIYIDGKQVDIRNCQQA
                     IAQGIAMVPEDRKRDGIVPVMAVGKNITLAALKKFTGSISQLDDAAEQKCILESIQQL
                     KVKTSSPDLAIGRLSGGNQQKAILARCLLLNPRILILDEPTRGIDIGAKYEIYKLINQ
                     LVQQGIAVIVISSELPEVLGLSDRVLVMHEGKLKANLINHNLTQEQVMEAALRSEHHV
                     EKQSV"
     gene            5815..6996
                     /gene="xylH"
                     /locus_tag="J1Y83_001981"
     CDS             5815..6996
                     /gene="xylH"
                     /locus_tag="J1Y83_001981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylose ABC transporter permease XylH"
                     /protein_id="HBA7630130.1"
                     /translation="MSKSNPSEVKLAVPTSGGLSGLKSLNLQVFVMIAAIIAIMLFFT
                     WTTDGAYLSARNVSNLLRQTAITGILAVGMVFVIISAEIDLSVGSMMGLLGGVAAICD
                     VWLGWPLPLTIIVTLVLGLLLGAWNGWWVAYRKVPSFIVTLAGMLAFRGILIGITNGT
                     TVSPTSAAMSQIGQSYLPASTGFIIGALGLMAFVGWQWRGRMRRQALGLQSPASTAVV
                     GRQALTAIIVLGAIWLLNDYRGVPTPVLLLTLLLLGGMFMATRTAFGRRIYAIGGNLE
                     AARLSGINVERTKLAVFAINGLMVAIAGLILSSRLGAGSPSAGNIAELDAIAACVIGG
                     TSLAGGVGSVAGAVMGAFIMASLDNGMSMMDVPTFWQYIVKGAILLLAVWMDSATKRR
                     S"
     gene            7074..8252
                     /locus_tag="J1Y83_001982"
     CDS             7074..8252
                     /locus_tag="J1Y83_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XylR family transcriptional regulator"
                     /protein_id="HBA7630131.1"
                     /translation="MFTKRHRITLLFNANKAYDRQVVEGVGEYLQASQSEWDIFIEED
                     FRARIDKIKDWLGDGVIADFDDKQIEQALADVDVPIVGVGGSYHLAESYPPVHYIATD
                     NYALVESAFLHLKEKGVNRFAFYGLPESSGKRWATEREYAFRQLVAEEKYRGVVYQGL
                     ETAPENWQHAQNRLADWLQTLPPQTGIIAVTDARARHILQVCEHLHIPVPEKLCVIGI
                     DNEELTRYLSRVALSSVAQGARQMGYQAAKLLHRLLDKEEMPLQRILVPPVRVIERRS
                     TDYRSLTDPAVIQAMHYIRNHACKGIKVDQVLDAVGISRSNLEKRFKEEVGETIHAMI
                     HAEKLEKARSLLISTTLSINEISQMCGYPSLQYFYSVFKKAYDTTPKEYRDVNSEVML
                     "
     gene            complement(8360..9184)
                     /locus_tag="J1Y83_001983"
     CDS             complement(8360..9184)
                     /locus_tag="J1Y83_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein bax"
                     /protein_id="HBA7630132.1"
                     /translation="MILTPIRRYGAMILMLLTLVFSSEVLAKTHTTTASQKSHLTKAS
                     NKQVSSKQEYSRNSAKSSSLPDLRKYPSGTPRKKAFLRTVMPYITSQNAAITAERNWL
                     ISKQYQGQWSPAERARLKDIAKRYKVKWSGNTRKIPWNTLLERVDIIPTSMVATMAAA
                     ESGWGTSKLARNNNNLFGMKCMKGRCTNAPGKVKGYSQFSSVKESVSAYVTNLNTHPA
                     YSSFRKSRAQLRKADQEVTATAMIHKLKGYSTKGKSYNNYLFAMYQDNQRLIAAHM"
     gene            9504..11534
                     /gene="malS"
                     /locus_tag="J1Y83_001984"
     CDS             9504..11534
                     /gene="malS"
                     /locus_tag="J1Y83_001984"
                     /EC_number="3.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HBA7630133.1"
                     /translation="MKLAACFLTLLPGFAVAASWTSPGFPAFSEQGTGTFVSHAQLPK
                     GTRPLTLNFDQQCWQPADAIKLNQMLSLQPCSNTPPQWRLFRDGEYTLQLDTRSGTPT
                     LMISIQNAVEPVASLVRECPKWDGLPLTLDVSATFAEGAAVRDYYSQQIAIVKNGQIT
                     LQPAATSNGLLLLERAETDASAPFDWHNATVYFVLTDRFENGDPSNDQSYGRHKDGMA
                     EIGTFHGGDLRGLTNKLDYLQQLGVNALWISAPFEQIHGWVGGGTKGDFPHYAYHGYY
                     TQDWTNLDANMGREADLRTLVDSAHQRGIRILFDVVMNHTGYATLADMQEFQFGALYL
                     SGDEVKKTLGERWSDWKPAAGQTWHSFNDYINFSDKTGWDKWWGKNWIRTDIGDYDNP
                     GFDDLTMSLAFLPDIKTESTTASGLPVFYKNKTDTHAKVIDGFTPRDYLTHWLSQWVR
                     DYGIDGFRVDTAKHVELPAWQQLKTEASAALREWKKANPDKALDDKPFWMTGEAWGHG
                     VMQSDYYRHGFDAMINFDYQEQAAKAVDCLAQMDTTWQQMAEKLQDFNVLSYLSSHDT
                     RLFREGGNKAAELLLLAPGAVQIFYGDESSRPFGPTGSDPLQGTRSDMNWQDVSGKSA
                     ASVAHWQKISQFRARHPAIGAGKQTTLSMKQGYGFVREHGDDKVLVIWAGQQ"
     gene            11712..12965
                     /gene="avtA"
                     /locus_tag="J1Y83_001985"
     CDS             11712..12965
                     /gene="avtA"
                     /locus_tag="J1Y83_001985"
                     /EC_number="2.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--pyruvate transaminase"
                     /protein_id="HBA7630134.1"
                     /translation="MTFSLFGDKFTRHSGITLLMEDLNDGLRTPGAIMLGGGNPAQIP
                     EMQDYFQTLLTDMLESGKATDALCNYDGPQGKTELLTLLAGMLREKLGWDIEPQNIAL
                     TNGSQSAFFYLFNLFAGRRADGRIKKVLFPLAPEYIGYADAGLEEDLFVSARPNIELL
                     PEGQFKYHVDFEHLHIGEETGMICVSRPTNPTGNVITDEELLKLDALANQHGIPLVID
                     NAYGVPFPGIIFSEARPLWNPNIVLCMSLSKLGLPGSRCGIIIANEKIITAITNMNGI
                     ISLAPGGIGPAMMCEMIKRNDLLRLSETVIKPFYYQRVQETIAIIRRYLPEDRCLIHK
                     PEGAIFLWLWFKDLPITTEQLYQRLKARGVLMVPGHNFFPGLDKPWPHTHQCMRMNYV
                     PEPEKIEAGVKILAEEIERAWAESH"
     gene            complement(13117..13590)
                     /locus_tag="J1Y83_001986"
     CDS             complement(13117..13590)
                     /locus_tag="J1Y83_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HBA7630135.1"
                     /translation="MNRFIIADATKCIGCRTCEVACAVSHQENQDCAALSPDEFISRI
                     RVIKDHSWTTAVACHQCEDAPCANVCPVDAISREHGHIFVEQSRCIGCKSCMLACPFG
                     AMEVVSSRKKARAIKCDLCWHRATGPACVEACPTKALQCMDVEKVQRHRLRQQPV"
     gene            complement(13692..14540)
                     /gene="yiaJ"
                     /locus_tag="J1Y83_001987"
     CDS             complement(13692..14540)
                     /gene="yiaJ"
                     /locus_tag="J1Y83_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator YiaJ"
                     /protein_id="HBA7630136.1"
                     /translation="MGKEVMGKKENEMAQEKERPAGSQSLFRGLMLIEILSNYPNGCP
                     LAHLSELAGLNKSTVHRLLQGLQSCGYVTTAPAAGSYRLTTKFIAVGQKALSSLNIIH
                     IAAPHLEALNIATGETINFSSREDDHAILIYKLEPTTGMLRTRAYIGQHMPLYCSAMG
                     KIYMAFGHPDYVKSYWESHQHEIQPLTRNTITELPAMFDELAHIRESGAAMDREENEL
                     GVSCIAVPVFDIHGRVPYAVSISLSTSRLKQVGEKNLLKPLRETAQAISNELGFTVRD
                     DQGAIT"
     gene            14741..15739
                     /gene="yiaK"
                     /locus_tag="J1Y83_001988"
     CDS             14741..15739
                     /gene="yiaK"
                     /locus_tag="J1Y83_001988"
                     /EC_number="1.1.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydro-L-gulonate 2-dehydrogenase"
                     /protein_id="HBA7630137.1"
                     /translation="MKVTFEQLKAAFNRVLISRGVDSETADACAEMFARTTESGVYSH
                     GVNRFPRFIQQLENGDIIPDAQPKRITSLGAIEQWDAQRSIGNLTAKKMMDRAIELAA
                     DHGIGLVALRNANHWMRGGSYGWQAAEKGYIGICWTNSIAVMPPWGAKECRIGTNPLI
                     VAIPSTPITMVDMSMSMFSYGMLEVNRLAGRQLPVDGGFDDEGNLTKEPGVIEKNRRI
                     LPMGYWKGSGMSIVLDMIATLLSDGASVAEVTEDNSDEYGISQIFIAIEVDKLIDGPT
                     RDAKLQRIMDYVTSAERADENQAIRLPGHEFTTLLAENRRNGITVDDSVWAKIQAL"
     gene            15751..16215
                     /locus_tag="J1Y83_001989"
     CDS             15751..16215
                     /locus_tag="J1Y83_001989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF386 domain-containing protein"
                     /protein_id="HBA7630138.1"
                     /translation="MIFGHIAQPNPCRLPAAIEKALDFLRTTDFNALEPGVVEIDGKN
                     IYAQIIDLTTREAVENRPEVHRRYIDIQFLAWGEEKIGIAIDTGNNKVSESLLEQRDI
                     IFYHDSEHESFIEMIPGSYAIFFPQDVHRPACNKNATTAIRKIVVKVALAAL"
     gene            16238..17158
                     /locus_tag="J1Y83_001990"
     CDS             16238..17158
                     /locus_tag="J1Y83_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000856281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4862 family protein"
                     /protein_id="HBA7630139.1"
                     /translation="MKTNNAGYIIGAYPCAPSFHQRSEEEEKDFWRQLSETPDILGLE
                     QPCLENFHPLGDQWLLRHTPESWKFVVTAVMETMRRRSENSGFGLASSDEEQRQACVA
                     YYRHLFNKINALQANKVLALALQAAPLATNPNVMQATDAFARSLKEIASWDWPCKLVL
                     EHCDAMTSSSPRKGFLPLENVLEVITDYDISICINWARSAIEGRNTTLPLTHTQMAKQ
                     AGKLGALMFSGTTLNGAYGEWQDLHAPFAPFCAESLMTTDHVRELFNVAESSTLHFAG
                     IKLLEINATADVHHRIEILRNGIHSLNESR"
     gene            17272..17739
                     /gene="yiaM"
                     /locus_tag="J1Y83_001991"
     CDS             17272..17739
                     /gene="yiaM"
                     /locus_tag="J1Y83_001991"
                     /inference="COORDINATES: protein motif:HMM:NF016200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diketo-L-gulonate TRAP transporter small
                     permease YiaM"
                     /protein_id="HBA7630140.1"
                     /translation="MKRILEGILAIIIAILSCIIFINIILRYGFHTSILSIDELSRLL
                     FVWLTFIGAIVAYMDNSHVQVTFLVEKLSPANQQRVSLLTHTLILLLCMGLAWGAIEK
                     TAQDWSNLSPILGVPVGLMYAAAIPTSLIIALLELRHLYRQFTNTPSRNQQGA"
     gene            17544..19019
                     /gene="yiaN"
                     /locus_tag="J1Y83_001992"
     CDS             17544..19019
                     /gene="yiaN"
                     /locus_tag="J1Y83_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014640456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diketo-L-gulonate transporter large permease
                     YiaN"
                     /protein_id="HBA7630141.1"
                     /translation="MYGAGLGRYRKNSARLEQLIAHSWRPRWLNVRRRYPHQSDYRIA
                     GIASLIPAVHKYPQPKPTRSLTMTILIFIVSLLGAIAIGVPIAWALLLCGISLMFWMD
                     IFDVQILAQTLVNGADSFSLLAIPFFVLAGEIMNAGGLSQRIVDLPMKLVGHRPGGLG
                     YVGVLAAMIMASLSGSAVADTAAVAALLVPMMRQANYPVNRAAGLIGSGGIIAAIIPP
                     SIPLIIFGVSSGLSISKLFMAGIAPGIMMGVTLMVTWWWQAKRLNLPCQPKASLREVW
                     QSLVSGIWALFLPIIIIGGFRSGLFTPTEAGAVAAFYALFVSVVVYREMTFSTLYHVL
                     INAAKTTSVVMFLVASAAVSAWLITIAELPMMVSELLQPLVDSPRLLFIVIMLAIMVI
                     STVMDLTPTVLILTPVLMPLVKEAGIDPVYFGIMFIINCSISLITPPVGNVLNVVCGV
                     AKLKFDDAVKGVAPYVMVLFMLLALFIFIPELITAPLKWMS"
     gene            19032..20018
                     /gene="yiaO"
                     /locus_tag="J1Y83_001993"
     CDS             19032..20018
                     /gene="yiaO"
                     /locus_tag="J1Y83_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diketo-L-gulonate transporter
                     substrate-binding protein YiaO"
                     /protein_id="HBA7630142.1"
                     /translation="MKLRSVTYALLIAGVATFSTSSLAAQSLRFGYETSQTDSQHIAA
                     KKFNELLQEKTKGELKLKLFPDSTLGNAQAMISGVRGGTIDMEMSGSNNFTGLSPVIN
                     LLDVPFLFRDTAHAHKTLDGKVGDDLKVSLEGKGLKVLAYWENGWRDVTNSRAPVKTP
                     ADLKGLKIRTNNSPMNIAAFKVFGANPIPMPFAEVYTGLETRTIDAQEHPINVVWSAK
                     FYEVQKYLSLTHHAYSPLLVVINKAKFDGLTPEFQQALISSAQEAGSYQRKLVAEDQQ
                     KIIDGMKEAGVEVITDLDRKAFSDALGTQVRDMFVKDVPQGADLLKAVDEVQ"
     gene            20022..21518
                     /gene="lyxK"
                     /locus_tag="J1Y83_001994"
     CDS             20022..21518
                     /gene="lyxK"
                     /locus_tag="J1Y83_001994"
                     /EC_number="2.7.1.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-xylulokinase"
                     /protein_id="HBA7630143.1"
                     /translation="MTQYWLGLDCGGSWLKAGLYDREGREAGVQRLPLCALSPQPGWA
                     ERDMAELWQCCTAVIRALLTHSGVSGEQIVGIGISAQGKGLFLLDKNDKPLGNAILSS
                     DRRAMEIVRRWQEDGIPEKLYPLTRQTLWTGHPVSLLRWLKEHEPERYAQIGCVMMTH
                     DYLRWCLTGVKGCEESNISESNLYNMSRGEYDPCLTDWLGIAEINHALPPVVGSAKIC
                     GEITAQTAVLTGLKAGTPVVGGLFDVVSTALCAGLEDEFTLNAVMGTWAVTSGITHGL
                     RDGEAHPYVYGRYVNDGQFIVHEASPTSSGNLEWFTAQWGEISFAEINQAVASLPKAG
                     GDLFFLPFLYGSNAGLEMTSGFYGMQAIHTRAHLLQAIYEGVVFSHMTHLNRMRERFT
                     DVHTLRVTGGPAHSDVWMQMLADVSGLRIELPQVEETGCFGAALAARVGTGVYRDFSE
                     AQRDLQHPVRTLQPDMAAHQLYQQKYQRYQHLIAALQGYHTRIKEHTL"
     gene            21515..22177
                     /gene="ulaD"
                     /locus_tag="J1Y83_001995"
     CDS             21515..22177
                     /gene="ulaD"
                     /locus_tag="J1Y83_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HBA7630144.1"
                     /translation="MSRPLLQLALDHSSLEAAQRDVTRLKDSVDIVEAGTILCLNEGL
                     GAVKALREQCPDKIIVADWKVADAGETLAQQAFGAGANWMTIICAAPLATVEKGHAMA
                     QRCGGEIQIELFGNWTLDDARDWHRIGVRQAIYHRGRDAQASGQQWGEADLARMKALS
                     DIGLELSITGGITPADLPLFKEIRVKAFIAGRALAGAANPAQVAADFHAQIDSIWGGK
                     RA"
     gene            22170..23030
                     /locus_tag="J1Y83_001996"
     CDS             22170..23030
                     /locus_tag="J1Y83_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418039.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase"
                     /protein_id="HBA7630145.1"
                     /translation="MRNHPLGIYEKALAKDLSWPERLVLAKSCGFDFVEMSVDETDER
                     LSRLDWSAAQRASLVTAMIETGVAIPSMCLSAHRRFPFGSRDDAVRQRAREIMSKAIR
                     LARDLGIRTIQLAGYDVYYEDHDEGTQQRFAEGLAWAVEQAAASQVMLAVEIMDTAFM
                     NSISKWKKWDEMLASPWFTVYPDVGNLSAWGNDVPAELKLGIDRIAAIHLKDTQPVTE
                     HSPGQFRDVPFGEGCVDFVGIFKTLHELNYRGSFLIEMWTEKAKEPVLEIIQARRWIE
                     ARMQEAGFIC"
     gene            23024..23719
                     /gene="araD"
                     /locus_tag="J1Y83_001997"
     CDS             23024..23719
                     /gene="araD"
                     /locus_tag="J1Y83_001997"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HBA7630146.1"
                     /translation="MLEQLKADVLAANLALPVHHLVTFTWGNVSAIDETRQWMVIKPS
                     GVEYEVMTADDMVVVEIASGKVVEGSKKPSSDTPTHLALYRRFAEIGGIVHTHSRHAT
                     IWSQAGLDLPAWGTTHADYFYGAIPCTRLMTAEEINGEYEYQTGEVIIKTFEERGLNP
                     AKIPAVLVHSHGPFAWGKNAADAVHNAVVLEECAYMGLFSRQLAPQLPAMQNELLDKH
                     YLRKHGANAYYGQ"
     gene            complement(23766..25304)
                     /locus_tag="J1Y83_001998"
     CDS             complement(23766..25304)
                     /locus_tag="J1Y83_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001551836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HBA7630147.1"
                     /translation="MTNNPPSAQIKPGEYGFPLKLKARYDNFIGGEWVAPADGEYYQN
                     LTPVTGQLLCEVASSGKRDIDLALDAAHKVKDKWAHTSVQDRAAILFKIADRMEQNLE
                     LLATAETWDNGKPIRETSAADVPLAIDHFRYFASCIRAQEGGISEIDSETVAYHFHES
                     LGVVGQIIPWNFPLLMASWKMAPALAAGNCVVLKPARLTPLSVLLLMEIVGDLLPPGV
                     VNVVNGAGGEIGEYLATSKRIAKVAFTGSTEVGQQIMQYATQNIIPVTLELGGKSPNI
                     FFADVMDEEDAFFDKALEGFALFAFNQGEVCTCPSRALVQESIYERFMERAIRRVESI
                     RSGNPLDSVTQMGAQVSHGQLETILNYIDIGKKEGADVLTGGRRKLLEGELKDGYYLE
                     PTILFGQNNMRVFQEEIFGPVLAVTTFKTMEEALELANDTQYGLGAGVWSRNGNLAYK
                     MGRGIQAGRVWTNCYHAYPAHAAFGGYKQSGIGRETHKMMLEHYQQTKCLLVSYSDKP
                     LGLF"
     gene            complement(25412..26707)
                     /locus_tag="J1Y83_001999"
     CDS             complement(25412..26707)
                     /locus_tag="J1Y83_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HBA7630148.1"
                     /translation="MAIKSPPGLIPLSHLSGEELLAHLRFNRVTDEKGRYLPFDELQY
                     RIKKGENVDVAWTLTRLARNAAIQRINYCNEAGEQAGFNITPVIAEACELVDKRATAL
                     ALKDQTERLRGAGAELSQLRLEEPITSSQLEGANTTTLVARKMLETGRSPRTEDEHMI
                     AGNARLMAEIPHLLAEPLTPALIRQLHAIGMGGINDAKYRPGEFRETDDVVIADYDGN
                     IVHQPPAAALLPERLEKVCQWLNSHEGYIHPLVRACILHFMLAHEHPFRDGNGRTSRA
                     LFYWYMLKSGYDVFKYISISRLLHAAPVKYAASYQYTESDGMDLTYFLEYQAGVIKRA
                     LQNWQQHIDEITQRSAKLDSVLFSSGVLKRLNPRQVTLLNVMLANPGKEYTVAEISVS
                     LSVSDNTARTDLRTIVKEGFAQEKRINNQQAVYVAHYPL"
     gene            complement(26837..27988)
                     /gene="yiaY"
                     /locus_tag="J1Y83_002000"
     CDS             complement(26837..27988)
                     /gene="yiaY"
                     /locus_tag="J1Y83_002000"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine dehydrogenase"
                     /protein_id="HBA7630149.1"
                     /translation="MTASTFFIPSVNVIGADSLNDAMNMMADYGFTRTLIVTDNMLTK
                     LGMAGDVQKALEERNIFSVIYDGTQPNPTTENVAAGLKLLKENNCDSVISLGGGSPHD
                     CAKGIALVAANGGDIRDYEGVDRSAKPQLPMIAINTTAGTASEMTRFCIITDEARHIK
                     MAIVDKHVTPLLSVNDSSLMIGMPKSLTAATGMDALTHAIEAYVSIAATPITDACALK
                     AVTMIAENLPLAVEDGSNAKAREAMAYAQFLAGMAFNNASLGYVHAMAHQLGGFYNLP
                     HGVCNAVLLPHVQVFNSKVAAARLRDCAAAMGVNVTGKNDAEGAEACINAIRELAKKV
                     DIPAGLRDLNVKEEDFAVLATNALKDACGFTNPIQATHEEIVAIYRAAM"
BASE COUNT         6923 a         7219 c         7067 g         6867 t
ORIGIN      
        1 ggggataacg tttacgccat taatggcaga agttgctgat agaggcgacg gaacgtttct
       61 cgtcgtggct gataagcggc ataacgctgc gcatctggta gatgcgactg ttctaatggt
      121 agttgcggca acaactcaat gagcgatttc tctggattcg ccgcaatctg cgccagcctt
      181 gctgcgccca gtgctggccc cacatcgcca cccgtgcggt aatccagttg ctgaccgctg
      241 atgtccgcca gcatttgacg ccagtactca ctacgcgccc cgccaccaat caacgtaaca
      301 ctttgcggtt taatgccgca ggcatgcacg acatccatgc catctgccag cgcataaccc
      361 acgccttcca gcactgctcg cgccagttca ttggggccat gttgatgagt caaaccaaag
      421 aaaacccctt tcgcctgggg attattgtgt ggtgtacgct cgccggaaag ataaggcaga
      481 aaccaaactg gctcggcact ttcatcagcc tgtcgagctg cagtgattaa agcagggaca
      541 ttgctcaggc cggttaattt cgcggcccaa tccagacacg aagccgcact cagcattaca
      601 gacattaaat gccaacgttg cggtaacgca tggcaaaagc tatgtacggc gctttctggc
      661 ttgcttaaga acccttcgct gacagcaaaa tagacccccg acgtccccag cgataacatt
      721 gcctgattag catcaaccat tcccacacca accgcaccag ctgcattgtc gccaccgcct
      781 gcgataactg gcaccgccgc cataccccac gctttcgcaa cttcaggtaa caaagcacca
      841 gtaatttcgc tgccttcgta taatgcgggc atctggtcac gagataagtg gcaagcctgc
      901 agcatgagat cgctccagtc acgctttgcg acatccagcc acatagtgcc agctgcgtca
      961 gacatatcgc tggcaaactc ccccgtcata cgcagacgca agtaatcttt cggtaataat
     1021 actttatcga tttgacggaa tatctccggc tcatgccgct gaacccatag caatttaggc
     1081 gcagtaaatc cgggcatcat caggttgccg gtaatcaccc gcgattgcgg aactcgcgct
     1141 tccagcaaag tgcactcttg cgcacagcgc ccgtcgttcc acaaaatggc agggcgtaac
     1201 acccgttgct gagcatccag taaggttgca ccgtgcatct gtccggcaat acctaatgct
     1261 ttaacgtctt gcagagaatg ctgaacgccc agagctttca ttgcgcgatc agttgcctgc
     1321 caccactgtt ccgggtcttg ttccgaccag agtggatgcg ggcgcgaaac ggtcagcttt
     1381 tccgtttgcg cagcaaccac ctcaccctgc tcgttgagca aaataacttt tacgcccgag
     1441 gtgccaagat ctatcccgat atacatatcg atcgttcctt aaaaaaatgc ccggtatcgc
     1501 taccgataac cgggccaacg gactgcacag ttagccgtta tttgtcgaac agataatgat
     1561 ttaccagatt ttccagttgc tcctggcgac cactctgatg caccggagac aaattatgtt
     1621 cctgagcata tttggctaaa tctgccagtg acatttggcc tttcaggatt tgctggccta
     1681 attcgctatt ccagccggaa taacgctgcg cgatgcgttt atccagctcg ccatcttcaa
     1741 tcatgcgcgc tgcaattttc agcgccaacg ccatcgtatc catcgcgccg atatgaccgt
     1801 aaaacagatc atatttatca gtactttgac gacgtacttt ggcatcgaag ttcagaccac
     1861 cggtggtgaa accgcctgct ttgagaattt catacatcac cagcgcattc tcttccacac
     1921 tgttcgggaa ctggtcggtg tcccagccca gttgcgcatc gccacggttg gcgtcgacag
     1981 aaccgaacag gccaagcgca atggcggtgg ctatttcatg atggaaagag tgacctgcca
     2041 gcgtcgcgtg gttagcttca atattaagtt taatctcttt ttccagacca aactgtttca
     2101 ggaagccata gaccgtcgca gcatcgtaat catattgatg tttggtcggt tcttgcggtt
     2161 tcggttcgat aagcaacgtg ccctggaaac cgattttatg tttatgctca accaccatct
     2221 gcataaagcg gcccagctgt tcacgctcct ggcgcaagtc ggtatttaac agcgtttcgt
     2281 aaccttcacg accaccccac aggacatagt tttcaccgcc caatttatgg gttgcttcca
     2341 tcgctgtaac aacttgcgtt gccgcccaac tgaagacttc aggatctggg ttcgtcgccg
     2401 cacccgcgcc atagcgaggg tttgtaaagc agttagcagt tccccacaac agcttcacgc
     2461 cgctctcttc ttgcttgcct gccaggacat caaccatttg cgcaaaatta ttgatgtact
     2521 cttttaacga cgcgccctca ggggaaacat ccacatcgtg gaagcaataa aatggcacat
     2581 gtaacttgtg gaaaaactca aatgcgacat ctgctttacg cttcgccaac gccagtgcct
     2641 caccaggctg ctgccacgga cgattaaacg cccccacacc aaacatatcc gccccgttcc
     2701 agcagaaggt gtgccagtag caggcggcaa aacgcaagtg ctcttccata cgcttgccca
     2761 acaccagttc gtcgggattg tagtgacgga atgctaacgg gtttgaggat tttgagcctt
     2821 cataacgaac gcgatcgagc tggtcaaaat aggcttgcat attgaactcc ataatcaggt
     2881 aatgccgcgg gtgatggatg atgtcgtaat attgggcact ccctttcagt tgctcaatta
     2941 tgttatttca cactgctatt gagataattc acaagtgtgc gctcgctcgc aaaataaaat
     3001 gggatgatga aactgggtaa ttcctcgaag agaaaaatgc aataagtaca attgcgcaac
     3061 aaaagtaaga tctcggtcat aaatcaagaa ataaaccaaa aatcgtaatc gaaagataaa
     3121 aatctgtaat tgttttcccc tgtttagttg ctaaaaattg gttacgttta tcgcggtgat
     3181 tgttacttat taaagctgtc ctctaactac agaaggccct acaccatgaa aataaagaac
     3241 attctactca ccctttgcac ctcacttctg cttaccaacg ttgcggcaca cgccaaagaa
     3301 gtcaaaatag gtatggcgat tgatgatctc cgtcttgaac gctggcaaaa agatcgagat
     3361 atttttgtga aaaaagcaga atctctcggc gcgaaagtat ttgtacagtc tgcaaatggc
     3421 aatgaagaaa cacaaatgtc gcagattgaa aacatgatta accggggtgt cgatgttctt
     3481 gtcattattc cttataacgg tcaggtatta agtaacgttg taaaagaagc caaacaagaa
     3541 ggtattaaag tattagctta cgaccgtatg attaacgatg cggatatcga tttttatatt
     3601 tctttcgata acgaaaaagt cggtgaactg caggcaaaag ccctggtcga tattgttccg
     3661 caaggtaatt acttcctgat gggcggctcg ccggtagata acaacgccaa gctgttccgc
     3721 gccgggcaaa tgaaagtatt aaaaccttat gttgattccg gaaaaattaa agtcgttggt
     3781 gaccaatggg ttgatggctg gttaccggaa aacgcattga aaattatgga aaacgcgcta
     3841 accgccaata ataacaaaat tgatgctgta gttgcctcaa acgatgccac cgcaggaggg
     3901 gcaattcagg cattaagcgc gcaaggttta tcagggaaag tagcaatttc cggccaggat
     3961 gcggatcttg caggtattaa acgtattgct gccggtacgc aaactatgac ggtgtataaa
     4021 cctattacat tgttggcaaa tactgccgca gaaattgccg ttgagttggg caatggtcag
     4081 gagccaaaag cggataccac actgaataat ggcctgaaag atgttccctc ccgcctgctg
     4141 acaccgatcg atgtgaataa aaacaacatc aaagatacgg tagttaaaga cggattccac
     4201 aaagagagcg agctgtaagc gttacgcccc ggcgcggagc gggggcgtga tttctctcca
     4261 tgccgcgtga attaattggc ttaggtggag tcgttatgcc ttatctactt gaaatgaaaa
     4321 atattaccaa aaccttcggc agtgtgaagg cgattgataa cgtcagcttg cggttgaatg
     4381 ctggcgaaat cgtctcactc tgtggggaaa atgggtcagg taaatcaacg ctgatgaaag
     4441 tgctgtgtgg tatttatccc catggttcct acgaaggcga aattattttt gcgggagaag
     4501 agattcaggc gagtcacatc cgcgataccg aacgcaaagg tatcgccatt attcaccagg
     4561 aattggcgct ggtgaaagaa ctgaccgtgc tggaaaatat cttcctgggt aacgaaataa
     4621 cccataatgg cattatggat tatgacctga tgacgctacg ctgtcagaag ctgctcgcac
     4681 aggtcagttt atccatttca cctgataccc gcgttggcga tttaggactt gggcaacaac
     4741 aactggttga aattgccaag gcacttaata aacaggtgcg tttgttaatt ctcgatgagc
     4801 cgacagcctc attaactgag caggaaacgt cggttttact ggatattatt cgcgatctac
     4861 aacagcacgg tatcgcctgt atttatattt cgcacaaact caacgaagtc aaagcgattt
     4921 ccgatacgat ttgcgttatt cgcgacggtc agcacattgg cacgcgtgat gctgctggaa
     4981 tgagtgaaga cgatattatc accatgatgg tcgggcgaga gttaaccgcg ctttacccta
     5041 atgaaccaca taccaccgga gatgaaatat tacgtattga acatctgacg gcatggcatc
     5101 cggttaatcg tcatattaaa cgagttaatg atgtctcgtt ttccctgaaa cgtggcgaaa
     5161 tactgggtat cgccggactc gttggtgccg gacgtaccga gactattcag tgcctgtttg
     5221 gtgtgtggcc cggacaatgg gaagggaaaa tttatattga tggcaaacag gtagatattc
     5281 gtaactgtca gcaagccatc gcccagggga ttgcgatggt acccgaagac agaaagcgcg
     5341 acggcatcgt tccggtaatg gcggttggta aaaatattac cctcgccgca ctcaaaaaat
     5401 ttaccggtag tattagccag cttgatgacg cggcagagca aaaatgtatt ctggaatcaa
     5461 tccagcaact caaagttaaa acgtcgtccc ccgaccttgc tattggacgt ttgagcggcg
     5521 gcaatcaaca aaaagcgatc ctcgctcgct gtctgttact taacccgcgc attctcattc
     5581 ttgatgaacc caccaggggt atcgatattg gcgcgaaata cgagatctac aaattaatta
     5641 accaactcgt ccagcagggt attgccgtta ttgtcatctc ttccgaatta cctgaagtgc
     5701 tcggccttag cgatcgtgta ctggtgatgc atgaagggaa actaaaagcc aacctgataa
     5761 atcataacct gactcaggag caggtgatgg aagccgcatt gaggagcgaa catcatgtcg
     5821 aaaagcaatc cgtctgaagt gaaattagcc gtaccgacat ccggtggcct ttccgggctg
     5881 aagtcactga atttgcaggt cttcgtgatg attgcagcta tcatcgcaat catgctgttc
     5941 tttacctgga ccaccgatgg tgcctactta agcgcccgta acgtctccaa cctgttacgc
     6001 caaaccgcga ttaccggcat cctcgcggta ggaatggtgt tcgtcataat ttctgctgaa
     6061 atcgaccttt ccgtcggctc aatgatgggg ctgttaggtg gcgtcgcggc gatttgtgac
     6121 gtctggctag gctggccttt gccacttacc atcattgtga cgttggttct gggactgctt
     6181 ctcggagcct ggaacggatg gtgggtcgcg taccgcaaag tcccttcatt tattgtcacc
     6241 ctcgcgggca tgttggcatt tcgtggcata ctcattggca tcaccaacgg cacgactgtt
     6301 tcccccacca gcgccgcgat gtcacaaatt gggcaaagct atctacctgc cagcaccggc
     6361 ttcatcattg gcgcgcttgg cttaatggct tttgttggct ggcaatggcg cggaagaatg
     6421 cgccgtcagg ctttgggttt gcagtctccg gcctctaccg cagtagtcgg tcgccaggct
     6481 ttaaccgcta tcatcgtatt aggcgcaatc tggctgttga atgattaccg tggtgttccc
     6541 actcctgttc tgctgctgac gttgctgtta ctcggcggaa tgtttatggc aacgcggacg
     6601 gcatttggac gacgcattta tgccatcggc ggcaatctgg aagcggcacg tctctccggg
     6661 attaacgttg aacgcaccaa acttgccgtg ttcgctatta acggattaat ggtagccatc
     6721 gccggattaa tcctcagttc tcgacttggc gctggttcac cttctgcggg aaatatcgcc
     6781 gaactggacg caattgcagc atgtgtgatt ggcggcacca gtctggcagg cggcgtcggg
     6841 agcgtggccg gagcggtaat gggggcattc atcatggctt cactggataa cggcatgagt
     6901 atgatggatg taccgacctt ctggcagtat atcgttaaag gtgcgattct gttgctggca
     6961 gtatggatgg actccgcaac caaacgccgt tcttgatttt gataaaaatt ttctcaaagc
     7021 cggtaacgta ttaccggttt tgagtttttg catgattcag caggaaaaga accatgttta
     7081 ctaaacgtca ccgcatcaca ttactgttca atgccaataa agcctatgac cggcaggtag
     7141 tagaaggcgt aggggaatat ttacaggcgt cacaatcgga atgggatatt ttcattgaag
     7201 aagatttccg cgcccgcatt gataaaatca aggactggtt aggagatggc gtcattgctg
     7261 acttcgacga caaacaaatc gagcaagcgc tggctgatgt cgacgtcccc attgttgggg
     7321 ttggtggctc gtatcacctt gccgaaagtt acccacccgt tcattacatt gccaccgata
     7381 actatgcgct ggttgaaagc gcatttttgc atttaaaaga gaaaggcgtt aaccgctttg
     7441 ctttttatgg tcttccggaa tcaagcggta aacgttgggc cactgaacgc gaatatgcat
     7501 ttcgtcagct tgtcgccgaa gaaaagtatc gcggagtggt ttatcagggg ttagaaaccg
     7561 cgccagagaa ctggcaacac gcgcaaaatc ggctggcaga ttggctacaa acgctgccac
     7621 cgcaaaccgg gattattgcc gttactgacg cccgggcgcg gcatattctg caagtatgtg
     7681 aacatctaca tattcccgta ccggaaaaat tatgcgtgat tggcatcgat aacgaagaac
     7741 tgacccgcta tctgtcgcgt gtcgcccttt cttcggtcgc tcagggcgcg cggcaaatgg
     7801 gctatcaggc ggcaaaactg ttgcatcgtt tattggataa agaagagatg ccgttgcaac
     7861 ggattttggt cccgccagtc cgcgtgattg aacgacgctc aacggattac cgttcgttga
     7921 ctgatcctgc ggttattcag gccatgcatt acatccgtaa tcatgcctgc aaagggatta
     7981 aagtagatca agtactcgat gcggtcggaa tctcacgctc caatctcgag aagcgtttta
     8041 aagaagaggt gggtgaaacc atccatgcca tgattcatgc cgagaagctg gagaaagcgc
     8101 gcagtctgct gatttcaacc accttgtcga tcaatgagat atcgcaaatg tgcggttatc
     8161 catcgctgca atatttctac tctgttttta aaaaagcata tgacacaacg ccaaaagagt
     8221 atcgcgatgt aaatagcgag gtcatgttgt agcgatgatg agaattatcg gcgtcgcatc
     8281 aggtaactca gcacaaccac ctgatacgag caaaagcctt atccgcctga caagtcggat
     8341 aaggctcgag gaaatgcaat tacatatgcg ccgcgattaa ccgttggtta tcctggtaca
     8401 ttgcgaacag gtagttgtta taactcttcc ctttggtcga gtagcccttc agcttgtgaa
     8461 tcatcgctgt ggcagtcact tcctgatccg ctttacgcag ttgtgcacgc gatttacgga
     8521 acgaagagta agccgggtgc gtattgaggt tagtgacata ggcgctcacc gattctttga
     8581 cagaactaaa ctgtgagtac cctttcactt tacccggcgc attggtacaa cgtcctttca
     8641 tgcatttcat gccgaacagg ttgttgttgt tgcgcgccag ctttgacgtt ccccaaccgc
     8701 tttctgctgc agccatcgtc gccaccatac tggtagggat aatgtctacg cgttcaagca
     8761 aggtattcca cgggattttt cgcgtattac cagaccactt caccttgtag cgtttggcga
     8821 tgtctttcag acgcgcacgc tcagcaggtg accattggcc ctgatactgt tttgaaatca
     8881 gccagttacg ttccgcagta atggccgcat tttggctggt aatgtaaggc attacagtcc
     8941 ggagaaacgc ctttttcctt ggtgttccgg aagggtattt tcgcaaatca ggaagtgaac
     9001 tactctttgc actattgcga gaatactctt gtttactgct tacctgttta ttactagctt
     9061 tagttaagtg ggacttttga ctcgctgttg ttgtgtgcgt cttcgctaac acctcactcg
     9121 aaaacaccag agtgagtaac ataagaatca tcgccccata tcgtcgtatg ggagtcaaaa
     9181 tcatcaggtc tcctggtcgg atttaatcat tccaacacct tatatttttt acaaatttga
     9241 gagttgaatc tcaaatcata tcaaaaatag ctgtcaagag caccccaagg aatagtccaa
     9301 atctgaaact atgtcacgag ttaacgattc agattggcgc taaatcgcag aaaatgtggg
     9361 gtttatcgca aaattcagcc gttttttgcg cgcgatcgct cacccttgct tctcatcctg
     9421 tggacttacc gctcagggat gagttttgtc tggcttatcg ctggcaaact gtctgaaatc
     9481 gcagcaataa ggactcatcc gccatgaaac tcgccgcctg ttttctgaca ctccttcctg
     9541 gcttcgccgt tgccgccagc tggacttctc cggggtttcc cgcctttagc gaacagggga
     9601 cgggaacatt tgtcagccac gcacagttgc ccaaaggtac gcgtccacta acgctaaatt
     9661 ttgaccaaca gtgctggcag cctgcggatg cgataaaact caatcagatg ctttccctgc
     9721 aaccttgtag caacacgccg cctcaatggc gattgttcag ggacggcgaa tatacgctgc
     9781 aactagacac ccgctccggt acaccaacat tgatgatttc catccagaac gccgtcgaac
     9841 cggtagcaag cctggtccgt gaatgcccga aatgggatgg attaccgctc acactggatg
     9901 tcagcgccac ttttgcggag ggagccgccg tccgggatta ttacagccag caaattgcga
     9961 tagtgaagaa cggtcaaata acgttacaac ccgctgccac cagcaacggt ttactcctgc
    10021 tggaacgggc agaaactgac gcctctgccc ctttcgactg gcataacgcc acggtttact
    10081 ttgtgctgac agatcgtttc gaaaacggcg atcccagtaa tgaccagagt tacggacgtc
    10141 ataaagacgg tatggcagaa attggcactt ttcacggcgg cgatttacgc ggcctgacca
    10201 acaaactgga ttacctccag cagctgggcg tcaatgcttt atggataagc gccccatttg
    10261 agcaaattca cggctgggtc ggcggcggta caaaaggcga tttcccgcat tatgcctacc
    10321 acggttatta cacacaggac tggacgaatc ttgatgccaa tatgggccgc gaagccgatc
    10381 tacggacgct ggttgatagc gcgcatcagc gcggtattcg tattctcttt gatgtcgtga
    10441 tgaaccacac cggttatgcc acactggcgg atatgcagga gtttcagttt ggcgcgttat
    10501 atctttctgg tgacgaagtg aaaaaaacgc tgggtgaacg ctggagcgac tggaaacctg
    10561 ccgccgggca aacctggcat agctttaacg attacattaa tttcagcgac aaaacaggct
    10621 gggataaatg gtggggaaaa aactggatca gaaccgatat cggcgattac gacaatcctg
    10681 gattcgacga tctcactatg tcgctggcct ttttgccgga tatcaaaacc gaatcaacga
    10741 ccgcttctgg tctgccggtg ttctataaaa acaaaacgga tacccacgct aaagtcatcg
    10801 acggctttac ccctcgcgat tacttaaccc actggttaag tcagtgggtc cgcgactatg
    10861 ggattgatgg ttttcgggtc gataccgcca aacatgttga gttgcccgcc tggcaacaac
    10921 tgaaaaccga agccagcgcc gcgcttcgcg aatggaaaaa agcgaatccc gacaaagcat
    10981 tagatgataa accgttctgg atgaccggtg aagcctgggg ccacggcgtg atgcaaagtg
    11041 actactatcg ccacggcttc gatgcgatga tcaatttcga ttatcaggag caggcggcga
    11101 aagcagtcga ttgtctggcg cagatggata cgacctggca gcaaatggcg gagaaattgc
    11161 aggatttcaa cgtgttgagc tacctctcat cgcatgatac ccgcctgttc cgcgaaggtg
    11221 gtaataaagc agcagagtta ttgttattag cgccaggcgc ggtacaaatc ttttatggtg
    11281 atgaatcctc gcgtccgttc ggccccaccg gttcggatcc gctgcaaggt acgcgttcgg
    11341 atatgaactg gcaggatgtt agcggtaaat ctgccgccag cgtcgcgcac tggcagaaaa
    11401 tcagccagtt ccgcgcccgc catcccgcaa ttggcgcggg caaacaaacg acactttcga
    11461 tgaagcaggg ctacggcttt gttcgtgagc atggcgacga taaagtgctg gtcatctggg
    11521 ctgggcaaca gtgacttttc cggcttcccg ttcgtcagta cctcgggaag ccgccaacca
    11581 ggataaaatg tcagctctaa tcagcgttgc aggataaagc accgctccct cttcaacaga
    11641 gcgatttgca ccccagcaaa tgtagcgtta ttgttacctt ccttgctaca gagttcgaca
    11701 gatatcccgc tatgacattc tccctttttg gtgacaaatt tacccgccac tccggcatta
    11761 cgctgctgat ggaagatctg aacgacggtt tacgcacgcc tggcgcgatt atgctcggcg
    11821 gcggtaatcc agcgcagatc ccggaaatgc aggactactt ccagacgcta ctgaccgaca
    11881 tgctggaaag tggcaaagcg actgatgcac tgtgcaatta cgacggtcca caggggaaaa
    11941 cggagctcct cacactgctt gccggaatgc tgcgcgagaa gttgggttgg gatatcgaac
    12001 cacagaatat tgcactaaca aacggcagcc agagcgcgtt tttctactta tttaatctgt
    12061 ttgccggacg ccgtgccgat ggtcggatca aaaaagtgct gtttccgctg gcaccggaat
    12121 acattggcta cgctgacgcc ggactggaag aagatctgtt tgtctctgcg cgtccgaata
    12181 ttgaactgct gccggaaggc cagtttaaat accacgtcga ttttgagcat ctgcatattg
    12241 gcgaagaaac cgggatgatt tgcgtctccc ggccgacgaa tccaacagga aatgtgatta
    12301 ctgacgaaga gttgctgaag cttgacgcgc tggcgaatca acacggcatt ccgctggtga
    12361 ttgataacgc ttatggcgtc ccgttcccgg gtatcatctt cagcgaagcg cgcccgctat
    12421 ggaatccgaa tatcgtgctg tgcatgagtc tttccaagct ggggctacct ggctcccgct
    12481 gcggcattat catcgccaat gaaaaaatca tcaccgccat caccaatatg aacggcatta
    12541 tcagcctggc acctggcggt attggtccgg cgatgatgtg tgaaatgatt aagcgtaacg
    12601 atctgctgcg cctgtctgaa acagtcatca aaccgtttta ctaccagcgt gttcaggaaa
    12661 ctatcgccat cattcgccgc tatttaccgg aagatcgctg cctgattcat aaaccggaag
    12721 gagccatttt cctctggcta tggtttaagg atttgcccat tacgaccgag cagctctacc
    12781 agcgcctgaa agcacgcggc gtgctgatgg tgccggggca caacttcttc ccgggactgg
    12841 ataaaccgtg gccacatacg catcaatgta tgcgcatgaa ctacgtacca gagccggaga
    12901 aaattgaggc gggtgtgaag attctggcgg aagagataga aagagcctgg gctgaaagtc
    12961 actaaaaatt gcctgatgcg ctacgcttat caggcctaca tgatttctgc aatatattga
    13021 atttggtaga atttgtaggc cggataaggc gttttagccg catccggcat aaacaaagcg
    13081 cactatccct gcgttgagaa acgccggaaa aacgtttcaa acaggctgct gccgtagccg
    13141 atgccgctgc actttctcga catccatgca ctgcaacgcc tttgtcgggc aggcttcaac
    13201 acaggccggc cccgtcgccc gatgccagca cagatcgcac ttaatcgccc tcgccttttt
    13261 gcgcgaagaa acgacctcca tcgcaccaaa cgggcaagcc agcatacagc ttttacagcc
    13321 aatgcaacgt gattgttcaa cgaaaatatg cccatgttcg cggcttatcg cgtcaacagg
    13381 gcagacattc gcgcacggtg catcttcaca ctgatgacag gctactgccg tggtccagct
    13441 gtggtcttta atgacacgaa tacgggaaat aaactcgtct ggtgacaacg cagcgcaatc
    13501 ctgattctcc tgatgcgaca ctgcgcaagc cacttcacag gtacggcaac cgatacattt
    13561 cgtcgcatcc gcaataataa accggttcat gatcactcct ttgcactaaa acagcagagt
    13621 gccagagagc gcgtccctac tgctttgatc caacgaagga aatgttaaag ttttggcact
    13681 tgtccaaagg gttatgtgat cgcgccctgg tcgtccctga cagtaaatcc cagttcatta
    13741 gaaatagcct gcgcggtttc acgaagtggt ttcaggagat ttttctctcc cacctgtttc
    13801 agacgtgatg tcgaaagcga aatcgacacg gcgtacggca cccgcccatg aatatcaaac
    13861 accggaacag caatacagga gacgccgagt tcgttttctt ctctgtccat cgccgctcca
    13921 ctttcacgaa tgtgcgccag ttcgtcgaac atcgcgggca gctcggtaat ggtattgcgg
    13981 gttaacggct ggatctcatg ctgatggctt tcccagtatg acttcacgta gtccgggtga
    14041 ccaaacgcca tgtagatctt gcccattgcg gaacagtaga gcggcatatg ctggcctata
    14101 taggcacggg ttcgcagcat cccggttgtg ggttccagct tataaatcaa aatagcgtga
    14161 tcgtcttcgc ggctggagaa gttaatagtt tcaccagtgg cgatgttcag agcctcaaga
    14221 tgcggagcgg cgatatgaat gatattcagc gaagatagcg ccttctgccc gacggcaata
    14281 aatttggtgg tcaggcgata actccctgcg gcgggcgcgg tggtcacata gccgcaggac
    14341 tgtaatccct gcaataagcg atggacggta ctcttattta aaccagccag ctccgaaaga
    14401 tgcgccaacg gacaaccgtt tggatagttg ctcaaaatct caatcagcat caacccacga
    14461 aacagactct ggcttccggc tgggcgctct ttttcctgcg ccatctcgtt ctcttttttt
    14521 cccatcactt ctttccccat tttgtcgcgt cctgatggta gcgcaaagtg tgccgtagtt
    14581 cacgatctcg acagataatt tataaccact tgatttttaa gttttttgaa attcatcaat
    14641 cagattgctt tgttaaaaag tgatcgatat atttgaaatc aagtttcgta tattgaaatt
    14701 ttaagccaaa aaagcgatca aaaaaacaag gaagcctggg atgaaagtga catttgagca
    14761 gttaaaagca gcctttaatc gggtcttaat ttcacgcggc gttgacagcg aaacggctga
    14821 cgcctgtgca gagatgttcg cccgcaccac cgaatcaggc gtttattctc acggcgttaa
    14881 tcgtttccct cgtttcattc aacaactgga aaacggcgat atcattcctg atgcccaacc
    14941 caaacgtata accagcctcg gcgcaattga acagtgggac gcccagcgtt cgatcggtaa
    15001 cctgacagcg aaaaagatga tggatcgcgc cattgaactg gctgccgatc acggtattgg
    15061 tctggtggca ctacgtaatg ccaaccactg gatgcgcggc ggcagctacg gctggcaggc
    15121 ggcggaaaaa ggctatattg gcatttgctg gaccaactcc atcgccgtaa tgccaccgtg
    15181 gggcgcaaaa gagtgtcgca tcggcaccaa cccgctgatc gtcgccattc cttccacgcc
    15241 gatcaccatg gtcgatatgt cgatgtcgat gttctcttac ggcatgttag aagttaatcg
    15301 cctggctggt cgtcagctcc cggtagatgg tggctttgat gatgagggca atttgaccaa
    15361 agaacctggc gttatcgaga aaaatcgccg cattttgccg atgggctact ggaaaggttc
    15421 gggcatgtcg attgtgctgg atatgatcgc taccctcctt tctgacggcg catcggttgc
    15481 cgaagtcacc gaggacaaca gcgacgaata cggcatttca caaattttta ttgccattga
    15541 agtggacaag cttatcgacg gtcccacccg cgatgccaaa ctgcaacgca ttatggatta
    15601 cgttaccagt gccgagcgtg ctgacgaaaa ccaggctatt cgtttacccg gccacgaatt
    15661 tactaccctg ctggcagaaa accgccgtaa cggtattacc gttgatgaca gcgtgtgggc
    15721 aaaaatccag gcgttataag gagataagcc atgatttttg gacatatcgc gcagcctaat
    15781 ccgtgccgtt tgcccgccgc cattgaaaag gcgctcgatt ttctgcgcac caccgacttc
    15841 aacgccctgg aaccgggcgt tgtcgaaatc gacggcaaga atatttatgc gcaaattatc
    15901 gatttaacca cacgcgaggc ggtagaaaac cgtccggaag tgcatcgtcg gtatatcgat
    15961 attcagtttc tggcgtgggg tgaagagaaa atcggcattg ctattgatac gggaaataat
    16021 aaagtcagcg aatcactatt agagcagcgc gatattattt tttatcacga tagtgaacat
    16081 gaatcattta tagaaatgat tccgggcagc tacgctattt tcttcccgca ggatgttcat
    16141 cgcccggcat gtaataaaaa tgccactacg gcaatacgca agatcgtggt taaagtggcc
    16201 ctcgctgctt tataaaaaat gattactgga gcacataatg aaaacgaata atgccggtta
    16261 tattatcggc gcgtatccct gtgcaccttc ttttcaccaa agaagtgaag aggaagagaa
    16321 ggatttctgg cgacaattat ctgagactcc agacattttg gggctggaac aaccttgcct
    16381 ggaaaatttt caccctcttg gcgatcaatg gttactccgt catacgccag agagctggaa
    16441 atttgtcgtc accgcagtga tggaaaccat gcgtcgccgc agcgaaaaca gcgggtttgg
    16501 tctcgcctcc agtgatgaag aacagcgcca ggcctgcgtg gcgtactatc gccacctgtt
    16561 caataaaatc aacgcattgc aggctaataa agtgctcgca ctggcgctcc aggctgcacc
    16621 gttagcaacg aatcctaatg ttatgcaagc gacggacgct tttgcccgct cattgaaaga
    16681 aatcgccagt tgggactggc cgtgcaagct ggtgctggaa cactgcgacg ccatgacatc
    16741 ttcttccccc cgaaaaggct ttttgccttt agaaaatgtg ctggaagtta ttaccgatta
    16801 tgacatcagc atctgtatca actgggcgcg ctctgccatt gaagggcgca atacaacgct
    16861 gccgctgacc cacacccaaa tggcgaaaca agccggaaaa ctcggcgcgt tgatgttctc
    16921 tggtacgacg ctaaacgggg catacggcga atggcaggat ttacacgcac cgtttgcacc
    16981 attctgcgca gaaagcctga tgaccacaga tcatgttcgc gagttattta atgtagcaga
    17041 atcgtcaaca ttgcattttg ctgggatcaa gctactggaa ataaatgcga cggctgatgt
    17101 gcaccatcgc atcgaaatat tacgcaacgg cattcactct ctgaatgaat ctcgttaaac
    17161 agaaccattt atatatcact ttgaatatct aattagccgt tttctgataa ttattacgaa
    17221 tattaacaac attagcgcat aaaaaatatt gcataaccag aaggactcgt tatgaaacgg
    17281 atactcgaag gcattcttgc gatcatcatc gcaatactgt cttgtattat ttttattaat
    17341 atcattcttc gatatggatt ccacacaagt atattatcga ttgatgaatt atcccgactt
    17401 ttatttgtct ggttaacctt tattggcgca atcgttgctt atatggacaa cagccatgtg
    17461 caggttactt ttttagtcga aaaactctca ccggctaatc aacaacgtgt ttcgctgcta
    17521 acccatacgc tcattttatt gctgtgtatg gggctggcct ggggcgctat agaaaaaaca
    17581 gcgcaagact ggagcaactt atcgcccatt cttggcgtcc ccgttggctt aatgtacgcc
    17641 gccgctatcc ccaccagtct gattatcgca ttgctggaat tgcgtcactt ataccggcag
    17701 ttcacaaata cccccagccg aaaccaacaa ggagcctgac gatgacaatc cttatattta
    17761 ttgtcagttt gttaggggct atcgcaattg gcgttcccat cgcctgggca ttattactgt
    17821 gcggcatttc attgatgttc tggatggata tctttgatgt gcaaattctg gcacaaacgt
    17881 tggtaaatgg cgcagacagc ttctccctgt tagccatccc cttctttgta ctggcgggtg
    17941 agatcatgaa cgcgggcggc ctgtcgcaac gtatcgtcga cttgccaatg aaactggtag
    18001 gccacaggcc aggcggtctg ggttacgtgg gcgtgctggc agcgatgatc atggcaagcc
    18061 tgtccggttc tgcggttgct gataccgcag ctgtcgcggc gctgctggtg ccgatgatgc
    18121 gccaggccaa ctatccggta aaccgcgccg ccgggcttat cggttccggg gggattattg
    18181 cggccattat cccgccttcc ataccactca ttatcttcgg cgtctccagc ggactctcca
    18241 tcagcaaact gtttatggcg ggcattgcgc caggcatcat gatgggcgtc acgctgatgg
    18301 ttacctggtg gtggcaggca aaacgactga atttaccttg tcagccgaaa gcctcactgc
    18361 gagaagtctg gcaatcctta gtttccggta tttgggcgct ctttttaccg ataattatta
    18421 ttggcggttt ccgttcgggg ttatttacac cgaccgaagc aggcgcggtt gccgccttct
    18481 acgctctgtt cgtctcggtg gtggtctatc gcgagatgac cttctccacg ctttatcatg
    18541 tgctgattaa cgcggcaaaa accacatccg tggtgatgtt cctggtggcc tctgctgcgg
    18601 tatctgcatg gcttattacg atcgccgaat tacccatgat ggtatcggag ctattacaac
    18661 cgttggttga ttcgcctcgc ttattattca tcgtcattat gctcgctatt atggtgatca
    18721 gtacggtcat ggacttaacg cctaccgtat taattcttac accagtatta atgccattag
    18781 tgaaagaagc gggaatagat ccggtctatt tcggcatcat gtttattatc aactgttcaa
    18841 tatcactgat tacgcctcca gtcggtaatg ttcttaacgt ggtatgcggc gtggcgaaac
    18901 ttaaatttga cgatgcggta aaaggcgttg cgccttacgt catggtttta ttcatgttgt
    18961 tagcattatt tatttttata ccggagctca ttaccgctcc tcttaaatgg atgagttaaa
    19021 aggaaatcat aatgaaatta cgttctgtaa cctacgcttt attaattgcc ggagtggcga
    19081 cgttcagcac atcttctctg gcggcacaat cattacgttt cggttatgaa acctcacaaa
    19141 ccgattcaca acatattgcg gcgaaaaaat ttaatgaatt actgcaggaa aaaaccaaag
    19201 gcgaactgaa attaaaactg ttcccggata gcacccttgg taatgcgcaa gcgatgatca
    19261 gcggcgtgcg tggcggcact attgatatgg agatgtctgg ctctaacaac tttacgggac
    19321 tctcgcccgt catcaacctg ctcgatgtcc cgttcctgtt ccgcgatacc gctcacgcgc
    19381 ataaaacgct cgacggcaaa gtcggtgatg acctgaaagt ctcccttgaa ggtaaggggc
    19441 tgaaagtact ggcctactgg gaaaacggct ggcgcgatgt caccaactcg cgtgcaccgg
    19501 tcaaaacgcc tgccgacctg aaagggttga aaatccgcac caacaacagc ccgatgaata
    19561 tcgctgcatt taaagttttt ggcgctaacc cgatcccgat gccgtttgcc gaagtctata
    19621 ccgggctgga aacccgcact atcgacgctc aggaacaccc gatcaacgtc gtctggtcag
    19681 caaaatttta tgaagtgcag aagtaccttt ctctgaccca tcacgcctat tcgccgcttc
    19741 tggtggtgat caacaaagcg aagtttgatg gcttaacccc ggagttccag caagcactga
    19801 tctcatctgc acaggaagcg ggtagctatc agcgcaaact ggtcgccgaa gatcagcaaa
    19861 aaatcatcga cggtatgaaa gaagcgggcg tggaagtcat caccgatctc gaccgcaaag
    19921 cctttagcga tgcgctggga actcaggtcc gtgacatgtt tgttaaagat gttccccagg
    19981 gtgctgacct gctgaaagcc gtggatgagg tgcaataaac catgacgcaa tactggctgg
    20041 ggttagattg tggcggtagc tggctgaaag ccgggctgta tgaccgagaa ggccgggagg
    20101 cgggcgtgca gcgcctgccg ctgtgcgcat taagtccaca gccaggctgg gcagagcgcg
    20161 acatggcaga actgtggcaa tgttgcactg ctgtcattcg cgccctgctt acccattctg
    20221 gcgtcagcgg ggaacaaatt gtcggtatcg gcatctccgc gcagggaaag ggcttatttc
    20281 tacttgataa aaacgacaaa ccgctcggta acgccatttt gtcctcggat cgccgggcga
    20341 tggaaatcgt tcgtcgctgg caggaagatg gcatcccgga aaaactctat ccgctgaccc
    20401 gacaaacctt atggaccggg catccggtgt cgctgttacg ctggctgaaa gagcacgaac
    20461 cagaacgcta cgcgcaaatt ggctgcgtga tgatgacgca cgactacctg cgctggtgtt
    20521 taaccggcgt caaaggttgc gaagagagca atatttctga gtccaatctc tacaacatga
    20581 gtcgcgggga atatgacccg tgccttaccg actggctggg gattgctgaa atcaatcacg
    20641 ccctgccgcc tgttgtcgga tctgccaaaa tctgcgggga aatcaccgct cagacagccg
    20701 tactgaccgg tctgaaagcg ggtacccccg ttgtcggcgg cctgtttgat gtggtttcca
    20761 ccgcactctg cgccgggctc gaagacgaat tcaccctcaa tgcggtgatg gggacctggg
    20821 cggtgaccag cggcataacc cacggtttac gtgacggtga agcgcatccg tatgtctatg
    20881 gtcgctacgt taacgatggt caatttatcg ttcacgaagc cagccccacc tcttccggca
    20941 atctcgaatg gtttaccgca cagtggggag aaatctcatt tgctgagatt aatcaggccg
    21001 ttgccagttt gcctaaagcc ggtggcgatc tctttttcct gccgttcctg tacggcagca
    21061 acgccggact cgaaatgacc agcggtttct acgggatgca ggccattcac acccgtgcac
    21121 acctcttaca ggctatctat gaaggggtgg tgttcagcca tatgacccat ctcaaccgga
    21181 tgcgcgaacg ttttactgat gtgcacaccc tgcgcgtcac tggcggcccg gcgcactccg
    21241 atgtctggat gcaaatgctg gcggacgtca gcggtctgcg tatcgagctg ccgcaggtgg
    21301 aagaaaccgg ctgctttggt gcggcacttg ccgcccgcgt cggtaccggg gtttatcgcg
    21361 atttcagcga agcccaacgt gacttacagc acccggtacg caccctgcag ccggatatgg
    21421 ccgcgcatca gctttaccaa caaaaatacc aacgctatca acatctcatt gccgcacttc
    21481 agggttatca cacccgcatc aaggagcaca cattatgagt cgaccacttc tgcaactggc
    21541 cctcgaccac tcatcacttg aagccgcgca gcgcgacgtg acgcggttaa aagacagcgt
    21601 cgatattgtc gaagcaggca ccattctctg tttaaacgaa gggcttggcg cagtgaaagc
    21661 cttgcgcgaa cagtgccctg acaaaatcat cgttgccgac tggaaggtcg ccgacgcggg
    21721 tgaaacgctc gcgcaacagg catttggcgc aggcgctaac tggatgacta tcatctgcgc
    21781 cgcgccgctc gccacggtgg aaaaaggcca cgcgatggcg caacgctgcg gcggtgaaat
    21841 ccagatcgag ctgttcggca actggacgct ggacgatgcc cgtgactggc atcgtattgg
    21901 cgtgcggcag gccatttatc atcgtggtcg tgacgcgcag gccagcgggc aacagtgggg
    21961 cgaggccgat ctggcacgca tgaaggcgct ttccgatatc ggccttgagc tttccattac
    22021 cggcgggatt acccctgctg acctgccgct gtttaaagag atccgcgtga aagcgtttat
    22081 tgccggacgg gcgctggcag gcgcagcaaa tccggcgcaa gtggccgctg attttcatgc
    22141 gcaaatcgac tccatctggg gaggcaagcg tgcgtaatca tccgttaggc atttatgaaa
    22201 aagcgctggc gaaagatctc tcctggccgg agcggctggt gctggcaaaa agctgtggtt
    22261 ttgattttgt cgaaatgtcg gtggatgaaa ccgacgaacg gttgtcacgt cttgactgga
    22321 gcgccgcaca acgtgcttcg ctggtcactg cgatgatcga aacgggtgtt gctattccgt
    22381 caatgtgcct gtccgcacat cgtcgttttc cttttggtag ccgggacgac gccgtgcgcc
    22441 agcgggcgcg ggagattatg agtaaagcga ttcgcctggc gcgcgatctc ggcattcgta
    22501 ctattcaact ggcaggttac gacgtctatt acgaggatca tgacgaaggc acccagcaac
    22561 ggtttgctga agggctggcg tgggcggttg aacaggcagc ggcatcacaa gtcatgctgg
    22621 cggtggagat tatggacacc gcgtttatga actctatcag caaatggaaa aagtgggacg
    22681 aaatgcttgc gtcaccgtgg ttcaccgttt acccggacgt aggcaatctc agcgcctggg
    22741 gcaatgatgt tcccgccgaa ctgaaactgg gcatcgaccg tatcgccgcc atccacctga
    22801 aagacactca acccgttacc gaacatagcc ccggacagtt ccgcgatgtg ccgtttggcg
    22861 aaggctgtgt cgattttgtt ggcatcttca aaacgctgca tgagctgaac tatcgcggat
    22921 catttctgat tgaaatgtgg accgaaaaag ccaaagagcc ggtgctggag attattcagg
    22981 cgcggcgctg gattgaagcg cgtatgcagg aggccggatt tatatgttag agcaactgaa
    23041 agctgacgtg ctggcggcaa acctggcgct tcccgttcat catctggtga cgttcacctg
    23101 gggcaatgtc agcgcgatag acgaaacgcg gcaatggatg gtgattaagc cttccggcgt
    23161 tgagtatgag gtaatgaccg ccgacgatat ggtggtggtt gagatagcca gcggcaaggt
    23221 ggtggaaggc agcaaaaagc cctcttccga cacgccaacg catctggcgc tttaccgtcg
    23281 ctttgcggag attggcggca tagtgcatac ccactcgcgc cacgccacca tctggtcgca
    23341 ggccggactt gatctccccg cctggggcac tactcacgcc gattattttt acggagccat
    23401 cccctgcacg cgcctgatga ccgcagaaga gatcaacggc gagtatgaat accagaccgg
    23461 cgaagtgatc attaaaacct tcgaagagcg tgggttgaac ccggcaaaaa tcccggcggt
    23521 actggtgcat tctcacggcc cgttcgcctg gggaaaaaat gccgccgatg cggtgcataa
    23581 cgccgtggta ctggaagagt gcgcctatat gggtctgttc tcgcgccagc ttgcgccaca
    23641 gctccctgcg atgcaaaacg aactgctgga taagcactac ctgcgtaagc atggggccaa
    23701 tgcctattac gggcagtagc catacctgat agtgcccggc aatacacgcc gggcagcctt
    23761 tcacatcaga acagccccaa cggtttatcc gagtagctca ccagcaggca cttggtttgc
    23821 tggtaatgct caagcatcat tttgtgggtt tcacggccaa tgcctgattg tttgtagccg
    23881 ccaaacgccg catgtgccgg gtaagcgtga taacagttgg tccacacgcg ccctgcctga
    23941 atgccgcgcc ccatcttata ggccagatta ccgttgcggc tccagacgcc cgcgcccaga
    24001 ccatattgcg tatcgttcgc cagctccagc gcttcttcca tcgttttgaa ggtggttacc
    24061 gccagcaccg ggccaaaaat ctcctcctgg aacacgcgca tattgttctg accaaacaga
    24121 atcgtcggtt cgaggtagta gccgtctttc agttcgcctt ccagcagctt gcgtcgaccg
    24181 ccggtgagca catcagcgcc ctctttttta ccgatatcaa tgtagttgag gatggtttcc
    24241 agttgcccgt gagaaacctg cgcgcccatt tgcgtcacgc tgtcgagcgg gttaccgctg
    24301 cgaatgcttt cgacacggcg gatggcgcgt tccataaagc gttcgtagat agactcctgc
    24361 actaaagcac ggcttggaca ggtgcaaact tcgccctggt taaaggcaaa cagtgcaaag
    24421 ccttccagcg ctttatcgaa aaaggcatct tcttcatcca tcacatcagc aaagaagata
    24481 tttggcgatt tgccgcccag ctccagcgtc accggaataa tgttttgcgt ggcgtattgc
    24541 ataatttgtt gacccacttc cgttgagccg gtaaacgcca ctttggcgat gcgtttcgag
    24601 gtcgccagat attcgccaat ttccccacct gcgccgttga ccacgttcac cacgcccggc
    24661 ggcagtaaat ccccgacgat ttccattagc agcagtacag aaagcggagt aagacgtgcg
    24721 ggtttcagca ccacacagtt gccagccgcc agcgcgggtg ccattttcca gctcgccatc
    24781 agcagcggga agttccacgg gataatctgc cccaccacgc ctaacgactc atggaaatga
    24841 taggccacgg tttcgctatc aatttcactg atcccacctt cctgcgcccg aatacacgag
    24901 gcaaaatagc gaaaatgatc aatcgccagc ggcacgtcgg cagcactggt ttcgcgaatg
    24961 ggtttaccgt tatcccaggt ttcagcggtc gctaacagtt cgaggttctg ctccatccga
    25021 tcggcaatct taaacagaat cgccgcgcga tcctgcaccg aggtgtgcgc ccatttatct
    25081 ttcactttgt gcgcagcatc cagcgccaga tcgatgtctc gtttgcccga agacgccact
    25141 tcgcacagta gctgcccggt caccggcgtc aggttctggt aatactcgcc atcggcaggc
    25201 gctacccatt cgccaccgat aaagttgtcg tagcgggctt ttaatttgag ggggaaacca
    25261 tactcgccgg gcttaatctg tgctgaaggg ggattattgg tcatgatcgt ctccttgctg
    25321 tgtgaggtat aacaagggta gacgtgactg gcgaaatctt cgccagtcgg taacagcttt
    25381 acgacggcta tcacgaattt acgggcaaaa gctacagcgg gtagtgtgcg acgtacactg
    25441 cttgctggtt attaattctt ttctcttgcg cgaatccttc tttaacaatt gtccgtaaat
    25501 cggtccgcgc cgtattatcc gagacgctca gactcacact aatttcagca actgtgtact
    25561 ctttccccgg attggcgagc atcacattca ggagcgttac ctggcgcgga ttcaggcgtt
    25621 taagcacacc agagctaaac agcacactat cgagttttgc gcttcgttgt gtaatttcat
    25681 cgatatgctg ctgccagttt tgcagagcac gtttaatgac acccgcctga tattcaagga
    25741 aataagtcag atccatacca tctgattcag tgtactggta gctggcggca tattttactg
    25801 gcgctgcatg aagcaaacgg ctgatggaaa tatatttaaa aacatcgtag cccgatttca
    25861 gcatatacca gtaaaacagc gcccgactgg tgcgcccgtt gccatcccga aacggatgtt
    25921 catgggccag cataaaatgc agaatgcagg cacggaccaa cggatgaata tacccttcat
    25981 ggctgtttaa ccactggcaa actttttcca gccgttccgg taacaatgcc gcagcgggcg
    26041 gttgatgcac tatattgcca tcatagtcgg cgatcacaac atcatctgtt tcacgaaatt
    26101 ctcccggacg atatttcgcg tcattgattc cacccatacc aatggcatgt aactggcgaa
    26161 taagcgcagg tgtcagcggt tctgcgagta agtgcggaat ttccgccatc agccgggcat
    26221 ttcccgcaat catatgctcg tcttcagtgc gtggcgaccg ccctgtttcc agcatttttc
    26281 tcgccaccag cgttgtagta ttcgcacctt ccagctggga gctggtgatc ggctcttcca
    26341 ggcgtaactg gcttaactcg gctccggccc cgcgtaaccg ttcagtctga tctttcagcg
    26401 ccagcgcggt agcacgtttg tcgacaagct cgcacgcttc tgcaatcacc ggggtaatgt
    26461 tgaatccggc ctgttcaccc gcttcattgc aataattaat tcgttggatc gccgcgttgc
    26521 gcgccagacg ggtaagcgtc cacgccacat cgacgttttc tccttttttg atgcggtact
    26581 gcaactcatc aaaaggcaga taacgtcctt tttcatcggt aacccggtta aagcgcaggt
    26641 gggccaggag ttcctcaccc gataagtgtg ataaaggaat aagtccggga ggtgatttga
    26701 tagccatgtg cttatctcaa aaaagcgcaa tattgagata gtgtatagca ggaaagagaa
    26761 actatctcaa aaaaagcaag ttttgagata gttgaaaaag cggctgacaa ctcgccagcc
    26821 gttgtgggaa tgatgattac atcgctgcgc gataaatcgc cacaatttct tcgtgagttg
    26881 cctggatcgg gttagtaaag ccgcaggcat ctttcagggc attagtcgcc agaaccgcga
    26941 aatcttcttc tttcacgttc aggtcgcgta ggcctgccgg gatatccact ttcttcgcca
    27001 gttcacggat ggcgttaatg caggcttcag caccttccgc gtcgttttta cctgtcacgt
    27061 tcacgcccat tgcagcggca caatcacgca gacgtgcagc ggcgactttg ctgttgaata
    27121 cctgaacatg cggcagcaaa acggcgttac atacaccgtg tggcaggttg tagaaaccgc
    27181 ccagctggtg cgccatcgca tgaacataac ccagagaagc attattgaac gccataccgg
    27241 cgaggaactg ggcataagcc attgcttcac gcgctttagc attgctgcca tcttcaacgg
    27301 ctaacggcag gttttcggca atcatggtca cggctttcag tgcacaagcg tcagtgatcg
    27361 gcgtggcggc aatagaaaca tacgcttcga tagcgtgcgt taaggcatcc atacccgttg
    27421 cggcggtcag tgacttcggc ataccaatca tcagagagga gtcattgaca gaaagcagcg
    27481 gagttacatg tttatccaca attgccattt tgatgtgacg cgcttcgtca gtgatgatgc
    27541 agaaacgggt catttcagag gccgtacccg ccgtggtgtt gatggcgatc atcggcagct
    27601 gcggttttgc agagcggtca acgccttcat aatcgcggat gtcaccgccg ttggctgcaa
    27661 ccagcgcaat accttttgcg cagtcgtgtg gagaaccacc gcctaaggaa attacgctat
    27721 cgcaattatt ttctttaagt aatttcaaac ctgcggcgac gttttccgtg gtggggttag
    27781 gttgggtgcc atcataaata acgctaaaaa tattgcgttc ttccagtgct ttttgcacat
    27841 cacccgccat acccaatttc gttaacatgt tgtcggtgac aattaaggta cgggtaaatc
    27901 cataatctgc catcatattc attgcatcat tcaatgaatc agcaccaata acattcacgg
    27961 aaggaataaa aaaggttgaa gctgtcataa cacactcctg aaagtgtaaa aaagggcggg
    28021 cagcttactg aggattttca tttgaaaata tgatcaacat taccgtttat taaaat
//
LOCUS       DADSYM010000056        28064 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2415
            SAMN08039579-rid11723173.denovo.056, whole genome shotgun sequence.
ACCESSION   DADSYM010000056 DADSYM010000000
VERSION     DADSYM010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039579
            Sequence Read Archive: SRR6362533
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 28064)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28064)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980162.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 03:39:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,484
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 4,347
            CDSs (with protein)               :: 4,347
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 43 of 85
            Pseudo Genes (incomplete)         :: 37 of 85
            Pseudo Genes (internal stop)      :: 19 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28064
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039579-rid11723173.denovo.056"
                     /strain="LGE2415"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8302 N 95.9628 W"
                     /collection_date="2015-08-24"
     gene            82..345
                     /gene="yhcN"
                     /locus_tag="J1Y83_002001"
     CDS             82..345
                     /gene="yhcN"
                     /locus_tag="J1Y83_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417705.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide/acid stress response protein YhcN"
                     /protein_id="HBA7630150.1"
                     /translation="MKIKTTVAALSVLSVLSFGAFAADSIDAAQAQNREAIGTVSVSG
                     VASSPMDMREMLNKKAEEKGATAYQITEARSGDTWHATAELYK"
     gene            complement(400..672)
                     /locus_tag="J1Y83_002002"
     CDS             complement(400..672)
                     /locus_tag="J1Y83_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA7630151.1"
                     /translation="MNIYTFDFDEIESQEDFYRDFSQTFGLAKDKVRDLDSLWDVLMN
                     DVLPLPLEIEFVHLGEKTRRRFGALILLFDEAEEELEGHLRFNVRH"
     gene            complement(764..2731)
                     /gene="aaeB"
                     /locus_tag="J1Y83_002003"
     CDS             complement(764..2731)
                     /gene="aaeB"
                     /locus_tag="J1Y83_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump subunit AaeB"
                     /protein_id="HBA7630152.1"
                     /translation="MGIFSIANQHIRFAVKLATAIVLALFVGFHFQLETPRWAVLTAA
                     IVAAGPAFAAGGEPYSGAIRYRGFLRIIGTFIGCIAGLVIIIAMIRAPLLMILVCCIW
                     AGFCTWISSLVRIENSYAWGLAGYTALIIVITIQPEPLLTPQFAVERCSEIVIGIVCA
                     IMADLLFSPRSIKQEVDRELESLLVAQYQLMQLCIKHGDGEVVDKAWGDLVRRTTALQ
                     GMRSNLNMESSRWARANRRLKAINTLSLTLITQSCETYLIQNTRPELITDTFREFFDT
                     PVETAQDVHKQLKRLRRVIAWTGERETPVTIYSWVAAATRYQLLKRGVISNTKINATE
                     EEILQGEPEVKVESAERHHAMVNFWRTTLSCILGTLFWLWTGWTSGSGAMVMIAVVTS
                     LAMRLPNPRMVAIDFIYGTLAALPLGLLYFLVIIPNTQQSMLLLCISLAVLGFFLGIE
                     VQKRRLGSMGALASTINIIVLDNPMTFHFSQFLDSALGQIVGCVLAFTVILLVRDKSR
                     DRTGRVLLNQFVSAAVSAMTTNVARRKENHLPALYQQLFLLMNKFPGDLPKFRLALTM
                     IIAHQRLRDAPIPVNEDLSAFHRQMRRTADHVISTRSDDKRRRYFGQLLEELEIYQEK
                     LRIWQAPPQVTEPVHRLTGMLHKYQHALTDS"
     gene            complement(2737..3669)
                     /gene="aaeA"
                     /locus_tag="J1Y83_002004"
     CDS             complement(2737..3669)
                     /gene="aaeA"
                     /locus_tag="J1Y83_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump subunit AaeA"
                     /protein_id="HBA7630153.1"
                     /translation="MKTLIRKFSRTAITVVLVILAFIAIFNAWVYYTESPWTRDARFS
                     ADVVAIAPDVSGLITQVNVHDNQLVKKGQVLFTIDQPRYQKALEEAQADVAYYQVLAQ
                     EKRQEAGRRNRLGVQAMSREEIDQANNVLQTVLHQLAKAQATRDLAKLDLERTVIRAP
                     ADGWVTNLNVYTGEFITRGSTAVALVKQNSFYVLAYMEETKLEGVRPGYRAEITPLGS
                     NKVLKGTVDSVAAGVTNASSTRDDKGMATIDSNLEWVRLAQRVPVRIRLDNQQENIWP
                     AGTTATVVVTGKQDRDESQDSFFRKMAHRLREFG"
     gene            complement(3677..3880)
                     /locus_tag="J1Y83_002005"
     CDS             complement(3677..3880)
                     /locus_tag="J1Y83_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000051842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AaeX family protein"
                     /protein_id="HBA7630154.1"
                     /translation="MSLFPVIVVFGLSFPPIFFELLLSLAIFWLVRRVLVPTGIYDFV
                     WHPALFNTALYCCLFYLISRLFV"
     gene            4063..4992
                     /gene="aaeR"
                     /locus_tag="J1Y83_002006"
     CDS             4063..4992
                     /gene="aaeR"
                     /locus_tag="J1Y83_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator AaeR"
                     /protein_id="HBA7630155.1"
                     /translation="MERLKRMSVFAKVVEFGSFTAAARQLQMSVSSISQTVSKLEDEL
                     QVKLLNRSTRSIGLTEAGRIYYQGCRRMLHEVQDVHEQLYAFNNTPIGTLRIGCSSTM
                     AQNVLAGLTAKMLKEYPGLSVNLVTGIPAPDLIADGLDVVIRVGALQDSSLFSRRLGA
                     MPMVVCAAKSYLTQYGIPEKPADLSSHSWLEYSVRPDNEFELIAPEGISTRLIPQGRF
                     VTNDPMTLVRWLTAGAGIAYVPLMWVINEINRGELEILLPRYQSDPRPVYALYTEKDK
                     LPLKVQVVINSLTDYFVEVGKLFQEMHGRGKEK"
     gene            complement(5120..6565)
                     /gene="tldD"
                     /locus_tag="J1Y83_002007"
     CDS             complement(5120..6565)
                     /gene="tldD"
                     /locus_tag="J1Y83_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease TldD"
                     /protein_id="HBA7630156.1"
                     /translation="MSLNLVSEQLLAANGLKHQDLFAILGQLAERRLDYGDLYFQSSY
                     HESWVLEDRIIKDGSYNIDQGVGVRAISGEKTGFAYADQISLLALEQSAQAARTIVRD
                     SGDGKVQTLGAVEHSPLYTSVDPLQSMSREEKLDILRRVDKVAREADKRVQEVTASLS
                     GVYELILVAATDGTLAADVRPLVRLSVSVLVEEDGKRERGASGGGGRFGYEFFLADLD
                     GEVRADAWAKEAVRMALVNLSAVAAPAGTMPVVLGAGWPGVLLHEAVGHGLEGDFNRR
                     GTSVFSGQVGELVASELCTVVDDGTMVDRRGSVAIDDEGTPGQYNVLIENGILKGYMQ
                     DKLNARLMGMTPTGNGRRESYAHLPMPRMTNTYMLPGKSTPQEIIESVEYGIYAPNFG
                     GGQVDITSGKFVFSTSEAYLIENGKVTKPVKGATLIGSGIETMQQISMVGNDLKLDNG
                     VGVCGKEGQSLPVGVGQPTLKVDNLTVGGTA"
     gene            complement(6721..10521)
                     /locus_tag="J1Y83_002008"
     CDS             complement(6721..10521)
                     /locus_tag="J1Y83_002008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA2 domain-containing protein"
                     /protein_id="HBA7630157.1"
                     /translation="MRRLPGILLLTGAALVVIAALLVSGLRIALPHLDAWRPEILNKI
                     ESATGMPVEASQLSASWQNFGPTLEAHDIRAELKDGGEFSVKRVTLALDVWQSLLHMR
                     WQFRDLTFWQLRFRTNTPITSGGGNDSLEASHISDLFLRQFDHFDLRDSEVSFLTPSG
                     QRAELAIPQLTWLNDPRRHRAEGLVSLSSLTGQHGVMQVRMDLRDDEGVLSNGRVWLQ
                     ADDIDLKPWLGKWMQDNIALETAQFSLEGWMTIDKGDVTGGDVWLKQGGASWLGEKET
                     HTLSVDNLTAHITRENPGWQFSIPDTRITMDGKPWPSGALTLAWIPEQDVGGKDNKRS
                     DELRIRASNLELAGLEGVRPLVAKLSPALGDVWRSTQPSGKINTLALDIPLQAADKTR
                     FQASWSDLAWKQWKLLPGAEHFSGTLSGSVENGLLTASMKQAKMPYETVFRAPLEIAD
                     GQATISWLNNDKGFQLDGRNIDVKAKAVHARGGFRYLQPANDEPWLGILAGISTDDGS
                     QAWRYFPENLMGKDLVDYLSGAIQGGEADNATLVYGGNPQLFPYKHNEGQFEVLVPLR
                     NAKFAFQPDWPALTNLDIELDFINDGLWMKTDGVNLGGVRASNLTAVIPDYSKEKLLI
                     DADIKGPGKAVGPYFDETPLKDSLGATLQELQLDGDVNARLHLDIPLNGELVTAKGEV
                     TLRNNSLFIKPLDSTLKNLSGKFSFINGDLQSEPLTASWFNQPLNVDFSTKEGAKAYQ
                     VAVNLNGNWQPAKTGFLPAAVNEALSGSVAWDGKVGIELPYHAGATYNVELNGDLKNV
                     SSHLPSPLAKPAGEPLPVNVKIDGNLNSFELTGQAGADNHFNSRWLLGQKLTLDRAIW
                     AADSKTLPPLPEQSGVELNMPPMNGAEWLALFQKGAAESVGGAASFPQHITLRTPMLS
                     LGNQQWNNLSIVSQPTANGTLVEAQGREINATLAMRNNAPWLANIKYLYYNPSVAKTR
                     GDSTQSSPFPTTERINFRGWPDAQIRCAECWFWGQKFGHIDSDITIFGDTLTLTNGLI
                     DTGFARLTAEGEWVNNPGNERTSLKGKLRGQKIDAAAEFFGVTTPIRQSSFNVDYDLH
                     WRKAPWQPDEATLNGIIHTQLGKGEITEINTGHAGQLLRLLSVDALMRKLRFDFRDTF
                     GEGFYFDSIRSTAWIKDGVMHTDDTLVDGLEADIAMKGSVNLVRRDLNMEAVVAPEIS
                     ATVGVAAAFAVNPIVGAAVFAASKVLGPLWSKVSILRYHISGPLDDPQINEVLRQPRK
                     EKAQ"
     gene            complement(10589..12058)
                     /gene="rng"
                     /locus_tag="J1Y83_002009"
     CDS             complement(10589..12058)
                     /gene="rng"
                     /locus_tag="J1Y83_002009"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease G"
                     /protein_id="HBA7630158.1"
                     /translation="MTAELLVNVTPSETRVAYIDGGILQEIHIEREARRGIVGNIYKG
                     RVSRVLPGMQAAFVDIGLDKAAFLHASDIMPHTECVAGEEQKQFTVRDISELVRQGQD
                     LMVQVVKDPLGTKGARLTTDITLPSRYLVFMPGASHVGVSQRIESESERERLKKVVAE
                     YCDEQGGFIIRTAAEGVGEAELASDAAYLKRVWTKVMERKKRPQTRYQLYGELALAQR
                     VLRDFADAELDRIRVDSRLTYEALLEFTSEYIPEMTSKLEHYTGRQPIFDLFDVENEI
                     QRALERKVELKSGGYLIIDQTEAMTTVDINTGAFVGHRNLDDTIFNTNIEATQAIARQ
                     LRLRNLGGIIIIDFIDMNNEDHRRRVLHSLEQALSKDRVKTSVNGFSALGLVEMTRKR
                     TRESIEHVLCNECPTCHGRGTVKTVETVCYEIMREIVRVHHAYDSDRFLVYASPAVAE
                     ALKGEESHSLAEVEIFVGKQVKVQIEPLYNQEQFDVVMM"
     gene            complement(12048..12641)
                     /gene="yhdE"
                     /locus_tag="J1Y83_002010"
     CDS             complement(12048..12641)
                     /gene="yhdE"
                     /locus_tag="J1Y83_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphatase YhdE"
                     /protein_id="HBA7630159.1"
                     /translation="MTSLYLASGSPRRQELLAQLGVTFERIVTGIEEQRQPQESAQQY
                     VVRLAREKAQAGVAQTAQDLPVLGADTIVILNGEVLEKPRDAEHAAQMLRKLSGQTHQ
                     VMTAVALADSQHILDCLVVTDVTFRTLTDEDIAGYVASGEPLDKAGAYGIQGLGGCFV
                     RKINGSFHAVVGLPLVETYELLSNFNALREKRDKHDG"
     gene            complement(12650..13138)
                     /gene="mreD"
                     /locus_tag="J1Y83_002011"
     CDS             complement(12650..13138)
                     /gene="mreD"
                     /locus_tag="J1Y83_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001487551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HBA7630160.1"
                     /translation="MASYRSQGRWVIWLSFLIALLLQIMPWPDNLIVFRPNWVLLILL
                     YWILALPHRVNVGTGFVMGAILDLISGSTLGVRVLAMSIIAYLVALKYQLFRNLALWQ
                     QALVVMLLSLVVDIIVFWAEFLVINVSFRPEVFWSSVVNGVLWPWIFLLMRKVRQQFA
                     VQ"
     gene            complement(13138..14241)
                     /gene="mreC"
                     /locus_tag="J1Y83_002012"
     CDS             complement(13138..14241)
                     /gene="mreC"
                     /locus_tag="J1Y83_002012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HBA7630161.1"
                     /translation="MKPIFSRGPSLQIRLILAVLVALGIIIADSRLGTFSQIRTYMDT
                     AVSPFYFVSNAPRELLDGVSQTLASRDQLELENRALRQELLLKNSELLMLGQYKQENA
                     RLRELLGSPLRQDEQKMVTQVISTVNDPYSDQVVIDKGSVNGVYEGQPVISDKGVVGQ
                     VVAVAKLTSRVLLICDATHALPIQVLRNDIRVIAAGNGCTDDLQLEHLPANTDIRVGD
                     VLVTSGLGGRFPEGYPVAVVSSVKLDTQRAYTVIQARPTAGLQRLRYLLLLWGADRNG
                     ANPMTPEEVHRVANERLMQMMPQVLPSPDAMGPKLPEPATGITQPTPQQPATGNAVTA
                     PAAPTQPAANRSPQRATPPQSGAQPPARAPGGQ"
     gene            complement(14307..15350)
                     /locus_tag="J1Y83_002013"
     CDS             complement(14307..15350)
                     /locus_tag="J1Y83_002013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000913396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HBA7630162.1"
                     /translation="MLKKFRGMFSNDLSIDLGTANTLIYVKGQGIVLNEPSVVAIRQD
                     RAGSPKSVAAVGHDAKQMLGRTPGNIAAIRPMKDGVIADFFVTEKMLQHFIKQVHSNS
                     FMRPSPRVLVCVPVGATQVERRAIRESAQGAGAREVFLIEEPMAAAIGAGLPVSEATG
                     SMVVDIGGGTTEVAVISLNGVVYSSSVRIGGDRFDEAIINYVRRNYGSLIGEATAERI
                     KHEIGSAYPGDEVREIEVRGRNLAEGVPRGFTLNSNEILEALQEPLTGIVSAVMVALE
                     QCPPELASDISERGMVLTGGGALLRNLDRLLMEETGIPVVVAEDPLTCVARGGGKALE
                     MIDMHGGDLFSEE"
     gene            complement(15655..17595)
                     /gene="csrD"
                     /locus_tag="J1Y83_002014"
     CDS             complement(15655..17595)
                     /gene="csrD"
                     /locus_tag="J1Y83_002014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase E specificity factor CsrD"
                     /protein_id="HBA7630163.1"
                     /translation="MRLTTKFSAFVTLLTGLTIFVTLLGCSLSFYNAIQYKFSHRVQA
                     VATAIDTYLVSNDFSTLRPQITELMMSADIVRVDLLHGDKQVYTLARNGSYRPVGTND
                     LFRELSVPLIKHPGMSLRLVYQDPMGNYFHSLMTTAPLTGAIGFIILMLFLAVRWLQR
                     QLAGQELLETRATRILNGERGSNVLGTIYEWPPRTSSALDTLLCEIQNAREQHSRLDT
                     LIRSYAAQDMKTGLNNRLFFDNQLATLLEDQEKVGTHGIVMMIRLPDFNMLSDTWGHS
                     QVEEQFFSLTNLLSTFMMRYPGALLARYHRSDFAALLPHRTLKEAESIASQLIKAVDT
                     LPNNKMLDRDDMIHIGICAWRSGQDTEQVMEHAESATRNAGLQGGNSWAIYDDSLPEK
                     GRGNVRWRTLIEQMLSRGGPRLYQKPAVTREGRVHHRELMCRIFDGNEEVSSAEYMPM
                     VLQFGLSEEYDRLQISRLIPLLRYWPEENLAIQVTVESLIRPRFQRWLRDTLMQCEKS
                     QRRRIIIELAEADVGQHISRLQPVIRLVNALGVRVAVNQAGLTLVSTSWIKELNVELL
                     KLHPGLVRNIEKRTENQLLVQSLVEACSGTSTQVYATGVRSRSEWQTLIQRGVTGGQG
                     DFFASSQPLDTNVKKYSQRYSV"
     gene            17747..18721
                     /locus_tag="J1Y83_002015"
     CDS             17747..18721
                     /locus_tag="J1Y83_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HBA7630164.1"
                     /translation="MQALLLEQQDGKTLASVQTLDESRLPEGDVTVDVHWSSLNYKDA
                     LAITGKGKIIRNFPMIPGIDFAGTVRTSEDPRFHAGQEVLLTGWGVGENHWGGLAEQA
                     RVKGDWLVAMPQGLDARKAMIIGTAGFTAMLCVMALEDAGVRPQDGEVVVTGASGGVG
                     STAVALLHKLGYQVVAVSGRESTHEYLKSLGASRILPRDEFAESRPLEKQVWAGAIDT
                     VGDKVLAKVLAQMNYGGCVAACGLAGGFTLPTTVMPFILRNVRLQGVDSVMTPPARRA
                     QAWQRLVADLPESFYTQAAKEISLAEAPKFAEAIINNQIQGRTLVKVN"
     gene            18907..18978
                     /locus_tag="J1Y83_002016"
                     /pseudo
     CDS             18907..18978
                     /locus_tag="J1Y83_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310737.1"
                     /note="in Escherichia coli this inner membrane protein was
                     found to anchor the periplasmic catalytic oxidoreductase
                     YedY; sulfite oxidase activity not demonstrated; contains
                     heme; incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sulfoxide reductase heme-binding subunit YedZ"
     gene            19699..20169
                     /gene="accB"
                     /locus_tag="J1Y83_002017"
                     /gene_synonym="fabE"
     CDS             19699..20169
                     /gene="accB"
                     /locus_tag="J1Y83_002017"
                     /gene_synonym="fabE"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HBA7630165.1"
                     /translation="MDIRKIKKLIELVEESGISELEISEGEESVRISRAAPAASFPVM
                     QQAYAAPMMQQPAQSNAAAPATVPSMEAPAAAEISGHIVRSPMVGTFYRTPSPDAKAF
                     IEVGQKVNVGDTLCIVEAMKMMNQIEADKSGTVKAILVESGQPVEFDEPLVVIE"
     gene            20180..21529
                     /gene="accC"
                     /locus_tag="J1Y83_002018"
     CDS             20180..21529
                     /gene="accC"
                     /locus_tag="J1Y83_002018"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009483995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HBA7630166.1"
                     /translation="MLDKIVIANRGEIALRILRACKELGIKTVAVHSSADRDLKHVLL
                     ADETVCIGPAPSVKSYLNIPAIISAAEITGAVAIHPGYGFLSENANFAEQVERSGFIF
                     IGPKAETIRLMGDKVSAIAAMKKAGVPCVPGSDGPLGDDMDKNRAIAKRIGYPVIIKA
                     SGGGGGRGMRVVRGDAELAQSISMTRAEAKAAFSNDMVYMEKYLENPRHVEIQVLADG
                     QGNAIYLAERDCSMQRRHQKVVEEAPAPGITPELRRYIGERCAKACVDIGYRGAGTFE
                     FLFENGEFYFIEMNTRIQVEHPVTEMITGVDLIKEQLRIAAGQPLSIKQEEVHVRGHA
                     VECRINAEDPNTFLPSPGKITRFHAPGGFGVRWESHIYAGYTVPPYYDSMIGKLICYG
                     ENRDVAIARMKNALQELIIDGIKTNVDLQIRIMNDENFQHGGTNIHYLEKKLGLQEK"
     gene            complement(21621..22667)
                     /locus_tag="J1Y83_002019"
     CDS             complement(21621..22667)
                     /locus_tag="J1Y83_002019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000787050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HBA7630167.1"
                     /translation="MKNEIWSWIGDLSQVAGIKHYELRSGRAKGTEAFDVRTGAGLAF
                     TVVKDRALDIAWASYKDTALSFITPNGIVAPAFFESQGNGFLRSFYAGLLTTCGLSYI
                     GTPCEDNGETLGLHGRLAATPAEEVGYRTERTDDGIEFVINGKVRETRLFGENLTLER
                     TIRCRYGENVLRIEDKVTNHGFTRQPLQILYHFNYGWPLLSPQAEILLSAKSVTPRTP
                     HAAEGLTSHLEICTPQPGFDEQVYYLTLNSDSQGMSKVALVNAELGWGIYEKFDTMQL
                     PNFIQWKNLGAGEYVMGLEVSNSFPDGRDKERAQGRLPFIEPGETKKYCFELGIVDGD
                     AEISALKAEIAGYR"
     gene            complement(22664..23626)
                     /locus_tag="J1Y83_002020"
     CDS             complement(22664..23626)
                     /locus_tag="J1Y83_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000048618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HBA7630168.1"
                     /translation="MSKVFTIGEILVEIMASKIGQPFDQPGIWNGPYPSGAPAIFIDQ
                     VTRLGVPCGIISCVGNDGFGDINIHRLAADGVDIRGISVLPLEATGSAFVTYHNSGDR
                     DFIFNIKNAACGKLSAQHVDENILKDCTHFHIMGSSLFSFHMVDAVKKAVTIVKANGG
                     VISFDPNIRKEMLDIPEMRDALHFVLELTDIYMPSEGEVLLLSPHSTPERAIAGFLEE
                     GVKEVIVKRGNQGASYYSANEQFHVESYPVEEVDPTGAGDCFGGAWIACRQLGFDAHR
                     ALQYANACGALAVTRRGPMEGTSRLMEIETFIQRHDMSIREAAQ"
     gene            complement(23648..24637)
                     /locus_tag="J1Y83_002021"
     CDS             complement(23648..24637)
                     /locus_tag="J1Y83_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001525780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBA7630169.1"
                     /translation="MTEKTLASPKPSEIPIANNFKKVFRQYGGILSGMVVLIILFSFI
                     NDSFFTANNITNIILQVSIIAITAYGMTYVLLLGDIDLSVGSTIALIGTFAALGASWG
                     IPFILLVPLSIIAALALGMINGGLTAIAGIPSFIVTVATMGIFRGIAYIVTDGMPIMI
                     KDDAFLALGNGEFLYIPIPIWILIILLLINHFILTKTTFGRKIYITGGNKEAAIYSGI
                     NVTRLKIKVFMITAVLAGISGMILASRLYSGQPNSALSYELDAIAAAVLGGTSLNGGY
                     GTVVGTVIGALTIGVINNGMNLMNVPYFYQMVVKGLVILVAVYFDVRNKRKRS"
     gene            complement(24638..26137)
                     /locus_tag="J1Y83_002022"
     CDS             complement(24638..26137)
                     /locus_tag="J1Y83_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020245594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HBA7630170.1"
                     /translation="MTDVILDVSHIAKTFGHVQALKDITLSLRKGRVHTLLGENGAGK
                     STLMKILAGVYPPTQGTITLRGETITINNPQHSRQLGIAIIFQELSLSNNMTVAENIY
                     ANNEPRRFGIINDKKMLADCQNLLAELGIPLDPLEMVGNMSMAHRQLVEIAKALSYAA
                     DVVIMDEPTSSLSDNEAEILFNIIEKLKQRGCAVIYISHRMDEIMRISDDISVIRDGE
                     YIATHEKKNSDIQHLIAQMVGREMKNIWPARLGEKPDENVPAKLEVKNLSHPSLFKEV
                     SFAVRPGEVLGFFGLVGAGRSDVMKALFGLVSYHGTVLIDGKEVRIANPKQAIDHGIA
                     FVTENRKEEGLVLMHDVNMNTHHVAFQYNASRMGLINHRQEEAKTLQSIARMNTKVSS
                     VHQAVGALSGGNQQKIVLSKWLEKTPRILLLDEPTRGVDVGAKFEIYNVIRQLAAAGT
                     AIILVSSELPEVMALSDRLVVMRNKTIADIYSCENLTQTQVMTAATGVR"
     gene            complement(26198..27088)
                     /locus_tag="J1Y83_002023"
     CDS             complement(26198..27088)
                     /locus_tag="J1Y83_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001341904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HBA7630171.1"
                     /translation="MKNIKLLGSAMLVSLALFSQSSLAKEYKIGASLLTQQHPFYIDL
                     ANAMKEEAKKDNVNLSVSIANQDLNKQLSDVEDFITKKVDAIIISPVDSKGVQAAIIK
                     ADKAGIPVITVDVAAEGVPVVSHVATDNYAGGVEAGKLMGKLLHGKGTVAIISYPALQ
                     SVVARVDGFKKGLSDTPDIKIVAEQPGITRAEALTTAQNIMQANANLNGLFGFGDDAA
                     LAAVIAAKSAHNDNIKIIGFDGMKEARDAVDSEKTFAAVIRQYPDQMGAKAIDAAVDH
                     LNGKPVEKMIPVQPGVYTGK"
     gene            complement(27124..27978)
                     /locus_tag="J1Y83_002024"
     CDS             complement(27124..27978)
                     /locus_tag="J1Y83_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000275530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose bisphosphate family class II aldolase"
                     /protein_id="HBA7630172.1"
                     /translation="MYIISSKNMLKKAQSEGYAVPAFNIHNLETLQVVVESAAQLRSP
                     VMLAGTPGTYRYGGVGSLISLVQSLAREYNLPLVLHLDHHEESDDIFNKVRAGIRSVM
                     IDGSHLPFEDNIARVQEVTTFCHRFDVSVEAELGRLGGQEDDLRVDEKDSAYTSPAAA
                     AEFVQRTQIDSLAVAIGTAHGLYAHEPRLDFVRLEQIRQRVEIPLVLHGASGLSEQDV
                     QHCIRRGICKVNVATELKIAFADALKGYFYNNPAANDPRHYMQPAKAAMKEVVTRIIG
                     VCGSEGKI"
BASE COUNT         7037 a         7530 c         6678 g         6819 t
ORIGIN      
        1 caataattag tgtatacttg attttgtgat atgggtcacg aaacaaaggc ccagctaaaa
       61 gattatgtcg aggtaaaaat catgaaaatc aaaaccactg ttgctgcatt aagtgtgctt
      121 tctgttctct ctttcggtgc attcgctgcc gactccattg atgctgcaca agcacaaaat
      181 cgtgaagcaa tcgggaccgt atccgtaagt ggtgtggcgt cttcgccaat ggatatgcgt
      241 gaaatgctga acaaaaaagc ggaagagaaa ggcgcaacgg cctaccagat tactgaagct
      301 cgtagtggtg acacctggca cgctacggct gaactgtaca aataaaccct catcgtcttg
      361 tccgacgata ttgcccccgg ttcgggggct ttttttgcgc taatgacgaa cattaaaacg
      421 caaatgccct tccagctctt cctctgcctc atcaaacagc aatattaacg cgccaaaacg
      481 gcgacgcgtt ttctctccca gatgaacaaa ttcaatctca agtggtagcg gcaggacatc
      541 gttcattaac acatcccata gtgagtcgag atcgcgtacc ttatctttcg ccagaccaaa
      601 ggtttggcta aagtcacgat aaaaatcctc ctgactctca atctcatcaa aatcaaaggt
      661 ataaatattc atctgttgcc accgtcacgt ttcgcggcga gttctgccgt tcatcgctaa
      721 gtatagccat gaaaaaaccg acgcttcggg cgtcggtttt gacttaacta tcggtcaacg
      781 catgttgata cttatggagc atacccgtaa gccgatgtac tggttccgtc acctgcggtg
      841 gcgcttgcca gatgcgtagc ttttcctggt agatttccag ttcttccagc aactgaccaa
      901 agtaccgacg acgtttatca tcgctacggg tagatatcac atggtctgct gtgcggcgca
      961 tttgccggtg aaacgccgat aaatcctcgt taaccggaat cggcgcatca cgcaggcgct
     1021 ggtgcgcgat aatcatcgtc agcgccagac gaaatttcgg caaatcccct gggaacttat
     1081 tcatcagcaa aaacagctgc tgataaagtg ccggaaggtg attctcttta cgacgtgcca
     1141 cattggtagt catcgcggaa acagcggcag aaacaaactg attaagcagt acacgtccag
     1201 tcctgtcgcg cgatttatcc cgcaccagca aaataacggt gaacgcgagc acacagccga
     1261 cgatttgccc taatgcgctg tcgagaaatt gactgaaatg gaaagtcatc gggttatcca
     1321 gcacgataat gtttatggtg cttgccaacg cccccatcga gcccaatctc cgtttctgta
     1381 cttctatacc gaggaaaaat cccagcactg ccaggctaat acacagcagc aacatgctct
     1441 gttgggtatt agggataatc accaaaaagt agagcagccc taacggtagc gctgccagcg
     1501 tcccgtagat aaagtcgatc gccaccatgc gtggattcgg caaacgcatt gccagtgacg
     1561 tcactaccgc aatcatcacc attgcaccac tgccggaagt ccagcccgtc cacagccaga
     1621 aaagcgtgcc cagaatgcaa gaaagtgtgg ttcgccagaa gttaaccatt gcatgatgac
     1681 gttcggctga ctctactttt acttccggtt cgccttgcag gatctcttct tcggtggcgt
     1741 tgatttttgt gttactgata acgccgcgct tgagaagctg ataacgcgtt gccgccgcga
     1801 cccagctata aatggtgaca ggcgtttccc gttccccggt ccaggcgatc actctccgca
     1861 ggcgtttgag ctgcttgtgg acgtcctgcg cagtttctac cggtgtgtca aaaaattcgc
     1921 ggaaagtatc agtgatcaat tccgggcgcg tattctgaat aagataagtt tcgcaggatt
     1981 gtgtaatcag cgtcagcgat agcgtattga tcgcttttaa acgtcgattg gcccgcgccc
     2041 agcgggaaga ttccatattc aggttgctgc gcataccttg taacgccgta gtacgtcgca
     2101 ccagatcgcc ccaggcttta tcgacaactt caccatcgcc atgcttgata cagagttgca
     2161 ttaattgata ttgcgcgacc agcaaacttt ccagctctcg atccacttct tgtttgatcg
     2221 atcgcggaga aaagagcaaa tccgccataa ttgcacacac aataccgata acgatctcgc
     2281 tacaacgttc gacggcaaac tgcggcgtaa gcaatggttc cggctgaatg gtgatcacaa
     2341 tgatcagcgc ggtataaccg gccagccccc acgcatacga gttttctatt cgtaccagcg
     2401 aggatatcca ggtacaaaaa ccggcccaga tacagcacac cagaatcatc aataatggtg
     2461 ctcggatcat cgcaatgatg attaccagtc cggcaataca gccaataaat gtgccgatga
     2521 tgcgcaaaaa gccacgatag cgaatagcgc cagaatacgg ttcacctccc gcagcaaagg
     2581 ccggaccggc ggcaacaatc gccgctgtca gtaccgccca gcgtggcgtt tccagctgga
     2641 agtgaaagcc aacaaacagc gccagtacaa tggcggtcgc cagttttacc gcaaagcgaa
     2701 tatgttgatt agcaatggag aaaataccca tcgtgattaa ccaaactcac gcaggcgatg
     2761 agccatttta cggaagaacg aatcctgact ttcgtcgcga tcttgtttgc cagtgactac
     2821 caccgtagca gtggtgcccg caggccagat gttctcttgc tggttgtcga gacgaatacg
     2881 taccggaaca cgttgcgcca aacgtaccca ttcaaggtta gagtcgatgg tcgccatccc
     2941 tttgtcgtca cgcgtgctgc tggcattggt gacccctgcg gcaacactat caacagtccc
     3001 tttcagcact ttgttactgc caagcggcgt gatctccgca cgatacccag ggcgcacccc
     3061 ttccagctta gtttcttcca tataggccag tacatagaag gagttctgtt tcaccagcgc
     3121 aaccgccgtt gatcctcgag taataaactc accggtatag acgttgaggt tggtcaccca
     3181 gccatctgct ggcgcgcgga tgactgtacg ttcaagatcc agttttgcca gatcgcgggt
     3241 cgcctgcgct ttcgctaact gatgcagaac tgtttgtagt acgttgttgg cctggtcgat
     3301 ctcttcgcga gacatcgcct gcacaccgag acggttacga cgtccggcct cctggcgttt
     3361 ctcctgtgcc agtacctgat aataagcaac atcggcttgc gcttcctcaa gcgccttttg
     3421 ataacgcggc tggtcgatgg tgaacagtac ctgtcctttt ttcaccagct ggttatcatg
     3481 aacattcacc tgggtaatga gtccagaaac gtccggcgcg atcgcaacga cgtcagcgct
     3541 aaagcgcgcg tcacgcgtcc acggggattc ggtgtaatag acccaggcat taaaaattgc
     3601 gatgaaggcc agaatgacta atacgaccgt gatggccgta cgggagaatt ttcttattag
     3661 tgttttcact tcaacctcaa acgaacagtc gcgatatcaa ataaaacaag cagcaataga
     3721 gcgcggtgtt gaacaacgcc ggatgccaga caaagtcgta aatacctgtt ggcacaagta
     3781 cccggcgcac cagccagaaa atcgccagtg ataaaagcaa ttcgaaaaat atcggtggga
     3841 aggacagccc aaacaccacg ataacgggaa acagactcat gttgaccttg gttgtataga
     3901 gagagcaggc gttattattt tcagcatctg tcgccgcaga gaagggcatg gaaagccggg
     3961 cgagagcaac attgctgtag attgatattt aatatattaa cgtaactgtt atgctgttat
     4021 ctatattatg tgatctaaat cacttttaag tcagagtgaa taatggaacg actaaaacgc
     4081 atgtcggtgt ttgccaaagt agttgaattt ggctctttta ccgccgccgc cagacagcta
     4141 cagatgagcg tttcgtccat cagtcagacg gtgtcaaaac tggaagatga gttgcaggta
     4201 aagctgttaa accgtagcac acgcagcatt ggcctgaccg aagccggtag aatttactac
     4261 cagggctgcc gtcggatgct ccatgaagtg caggatgttc atgagcaact gtatgccttc
     4321 aataacaccc ccatcgggac gctgcgcatt ggctgttctt caactatggc acaaaatgtt
     4381 ctcgccgggc tgacagcaaa aatgctgaaa gaatacccag gtttgagcgt caatctggtt
     4441 accggaattc ctgcccccga cctgattgcc gacggtctgg atgtggtgat ccgcgtcggc
     4501 gcgttgcaag attccagcct gttttcccgc cgtctgggcg cgatgccaat ggtggtgtgc
     4561 gccgcaaaaa gctatctcac acaatacggc ataccagaaa aacccgccga tttaagtagt
     4621 cattcatggc ttgaatacag cgtgcgaccc gacaatgaat ttgaactgat cgcaccggaa
     4681 gggatctcga ctcgcctgat cccgcaaggg agatttgtga ctaatgatcc gatgacgctg
     4741 gtgcgctggc tgacggcggg tgccgggatc gcctacgtgc cgttgatgtg ggtgatcaac
     4801 gagatcaatc gcggggaact ggagatcctg ctgccacgtt accagtcaga cccacgcccg
     4861 gtttatgcgt tatataccga aaaagataag ctgccgctga aggtacaagt cgtgatcaac
     4921 tcgctaacgg attattttgt tgaggtcggt aaattgtttc aggagatgca cgggcgcggg
     4981 aaagagaagt aattaatatt gtactgttac gttgtacaaa cctgtgccaa cgggttcccc
     5041 tcaccctaac cccagagggg cgaggggacc gttcgtacac ggagaaggat taattatcct
     5101 tctgaaaata ctgaaattat tacgcagtac cgccaacagt caggttatca actttcagcg
     5161 ttggctggcc cacgccaacc ggcaaacttt gcccttcttt accgcagaca cccacgccgt
     5221 tatccagttt caggtcgttg ccaaccatcg aaatctgctg catggtttcg ataccggaac
     5281 caatcaacgt tgcgcctttc accggcttag ttactttacc gttttcaata agatacgctt
     5341 ctgaggtgga gaaaacgaat ttgccggagg tgatatccac ctggccgcca ccaaagttcg
     5401 gtgcatagat accgtactca acggattcaa taatttcctg cggggtcgat ttacccggca
     5461 gcatataggt gttggtcata cgcggcatgg gcagatgagc gtaggattca cggcgaccgt
     5521 tgccagtcgg cgtcatcccc atcaaacgcg cgttgagttt atcctgcatg tagcctttca
     5581 gaatgccgtt ctcaatcagc acgttgtact ggcctggcgt accttcgtca tcaatcgcca
     5641 ccgaaccacg gcgatcgacc atcgtgccgt catcaaccac ggtacacagt tctgaagcca
     5701 ccaactcccc gacctgtcca ctaaatactg aagtgccacg gcggttgaag tcgccttcca
     5761 gaccgtgacc caccgcttca tgcaacagca cgcccggcca acctgcgcca agtactaccg
     5821 gcatggtgcc cgctggtgca gcaacggcag aaagattgac cagcgccata cgcactgctt
     5881 cttttgccca cgcatccgca cggacttcgc cgtcgagatc agcaaggaag aactcataac
     5941 caaaacgacc gccgccacca ctggcaccgc gttcgcgttt gccatcttct tcgaccaaaa
     6001 cgctcacgga aagacgcacc agcggacgaa catccgccgc tagtgtgccg tcggtggccg
     6061 caaccagaat taattcatag acaccactga ggctggcagt cacttcctgt acgcgcttgt
     6121 ccgcttcgcg ggcaacctta tcgacgcgac gcagaatatc caacttctct tcacggctca
     6181 tgctttgcag cggatctacc gaggtatata acgggctatg ctctaccgcg cccagcgtct
     6241 gtactttgcc atcaccacta tcacggacga tggtgcgcgc cgcttgtgca ctctgttcca
     6301 gcgccagcag gctgatttgg tcagcataag caaatccggt tttttcacca ctgattgcac
     6361 gcacgccaac gccctgatcg atgttgtaag aaccatcttt aataatacgg tcttctaaaa
     6421 cccaggattc gtgatagctc gactgaaaat agagatcgcc ataatcaagg cgacgttcgg
     6481 ccagttgacc gaggatcgcg aacaagtcct gatgtttcag gccgttcgcc gctagcaatt
     6541 gttcacttac caggttcaga ctcatcgttt ttgctactcg ttagttactg cagtagagga
     6601 ttttttacgg ctgccggatg cggcgtgaac gccctgtccg gcctacggtt atgttctggc
     6661 tcgttggcct ttggcaacga ttatcatatg agattggggc aattacgcgc cctcgtcaaa
     6721 tcattgcgct ttttctttgc gcggttgtcg caacacttcg ttgatttgcg gatcgtccag
     6781 cggacccgaa atgtgatagc gcaaaatgga gactttgctc cacagcggtc ccagcacttt
     6841 actggcggca aacactgccg cgccaacaat ggggttaacc gcaaaggccg cagccacgcc
     6901 caccgtcgca gaaatctctg gtgcgacaac cgcttccata ttcaggtcgc gacgtaccag
     6961 atttaccgac cctttcatag cgatgtccgc ttccaggcca tccaccagcg tgtcgtcggt
     7021 gtgcataacg ccgtctttaa tccacgcggt gctgcgaatg gagtcaaaat agaacccttc
     7081 gccaaaagtg tctctgaaat caaaacgcag cttacgcatc agggcatcta cgctcaataa
     7141 gcgcagcaat tgcccggcat gtccggtatt gatttcggta atttcgcctt tacccagttg
     7201 agtatgaatg atgccattca acgtcgcctc atctggctgc cagggtgctt tgcgccagtg
     7261 taaatcgtaa tccacattaa atgacgactg gcgtatgggc gtcgtgacac caaaaaattc
     7321 tgcggcggca tcaattttct gcccgcgcag ttttcctttc agcgaggttc gttcattccc
     7381 cggattgtta acccattcac cctcggcagt gagccgcgca aaaccagtat caatcagtcc
     7441 attagtcagc gttaacgtat cgccaaaaat ggtgatatca ctgtcaatgt gaccgaattt
     7501 ttgcccccag aaccagcact ctgcgcatcg tatttgtgcg tctggccagc cacggaagtt
     7561 aatgcgttcc gttgtcggga aaggtgatga ctgtgttgaa tcaccacgag ttttcgccac
     7621 gcttgggttg taataaagat atttgatatt cgccagccac ggtgcgttat tacgcatcgc
     7681 cagcgtggcg ttgatttcac gcccttgcgc ctcaaccagg gtgccatttg ccgtcggttg
     7741 cgaaacaata ctcaggttat tccattgctg atttcccagc gacaacatag gcgtacgtaa
     7801 cgttatgtgt tgtgggaaac tcgctgcacc accgacactc tccgccgcgc ctttctggaa
     7861 cagggccagc cactcggcac cattcatcgg cggcatattg agttcaacgc cactttgttc
     7921 cggcaacggc gggagcgttt tactgtctgc cgcccaaata gcacgatcga gcgtcagctt
     7981 ttgaccgagc aaccagcggc tattgaaatg gttatccgca ccagcctgtc cggttaattc
     8041 aaagctgttg agattgccat caatcttaac gtttaccggt agtggttcac ccgcaggttt
     8101 ggctaacggt gaaggtaagt gactgctcac attcttcaaa tcgccgttca gctctacgtt
     8161 atacgtcgcg ccagcatgat aaggcagctc aatgcccact ttaccatccc acgccacgct
     8221 gccacttaat gcttcgttca ccgctgcagg cagaaagccg gttttcgctg gttgccagtt
     8281 accgttgaga ttcaccgcga cctggtaggc ttttgcccct tctttggtgg aaaaatccac
     8341 gttcaacggc tgattaaacc agcttgctgt cagtggttca ctttgcagat cgccattgat
     8401 aaagctgaat ttaccgctca aatttttcag ggtgctgtcg agtggtttga taaacagact
     8461 gttattacgc agcgtcactt cccctttcgc ggttaccaac tcgccgttca gcgggatatc
     8521 aagatgtaag cgagcattca catcgccgtc gagctggagt tcttgcaggg tcgcacccag
     8581 agaatctttc agcggtgtct catcaaagta agggccaacg gctttacccg gacctttaat
     8641 gtcagcgtca atcagcagtt tttcttttga gtagtcaggg atcactgcgg tgagattact
     8701 cgcgcgcacg ccgcccaaat taacgccatc ggttttcatc cataaaccgt cgttaataaa
     8761 gtccagttca atatcaaggt tagttaatgc aggccagtcc ggctggaagg caaacttcgc
     8821 gttgcgcagc ggcaccagca cttcaaactg accttcgttg tgtttatagg ggaagagttg
     8881 cggattgcca ccataaacca gcgtcgcgtt atccgcttca ccgccctgaa tcgcgccact
     8941 taagtaatca accaggtctt tacccatcaa gttttccggg aaatagcgcc aggcttgtga
     9001 accatcatcg gtactgatgc cagccagaat acccagccag ggttcatcgt tagcaggttg
     9061 caggtaacga aaaccgccgc gcgcatggac ggctttggct ttaacgtcaa tattacgccc
     9121 atccagctgg aaacctttgt cattgttcag ccagcttatc gttgcctggc cgtcggcgat
     9181 ttccagtggc gcacggaata ccgtttcgta aggcatcttt gcctgcttca tcgacgcagt
     9241 aagcaaacca ttttctacgc tgccggaaag cgtcccggag aagtgttccg cacccggtaa
     9301 taatttccat tgcttccagg ccagatcgct ccacgatgcc tgaaaacggg tcttgtctgc
     9361 cgcctgaagc gggatatcca gcgccagagt gttaatcttg ccgctcggtt gtgtggagcg
     9421 ccaaacatca cccagtgcag gtgaaagttt cgcgaccagc gggcgtacgc cctccaggcc
     9481 tgccagctcc agattactgg cgcgaatccg gagttcgtca ctgcgtttat tgtctttgcc
     9541 gccaacgtcc tgttccggta tccaggccag cgtcaatgct ccgctcggcc agggtttgcc
     9601 gtccatcgtg atccgtgtat cgggaataga gaactgccat cccggatttt cacgcgtaat
     9661 atgcgcagtc agattatcca ccgacagcgt atgtgtttcc ttctcgccca accagctggc
     9721 accgccctgt ttcagccaga cgtcaccgcc ggttacatcg cctttgtcga tcgtcatcca
     9781 gccttcaagg gagaactgtg ccgtttccag cgcaatattg tcctgcatcc atttaccgag
     9841 ccacggcttc aggtcgatgt catccgcctg gagccagacg cgaccattgc ttaacacccc
     9901 ctcatcatcg cgcaaatcca tacgcacctg catcacgccg tgctgtccgg taaggctgga
     9961 gaggcttacc aggccttccg cacggtgtcg acgtggatcg ttcagccagg tgagctgtgg
    10021 gatcgccagc tcggcgcgct gaccggatgg cgtcaggaaa ctgacttcac tgtcgcggag
    10081 atcgaaatgg tcgaactgac gaagaaacag atcgctgatg tgactggctt ccaggctatc
    10141 attaccaccg ccgctggtga taggagtgtt ggtgcgaaag cgcagctgcc agaaagtgag
    10201 gtcgcgaaac tgccagcgca tatgtaacag gctctgccag acatccagtg ccagagtaac
    10261 gcgtttaacc gaaaattcgc cgccatcttt tagttctgca cggatgtcgt gcgcttcaag
    10321 cgtcggacca aaattctgcc agctggctga gagctgactg gcttctaccg gcatgccagt
    10381 cgcggattct attttgttga ggatttccgg acgccaggcg tcaagatgcg gtaaagcaat
    10441 acgcaggccg ctaaccagca gggcagcgat cacaacgagc gcggctccag taagcagtaa
    10501 aatccccggc aatcgcctca cccgtcactc cttgtctgct aaaaatgtga ctcaaaaacc
    10561 ctttgccgga tggcggccca gcatctgttt acatcattac gacgtcaaac tgctcctggt
    10621 tatagagcgg ttcaatttgt actttaacct gtttgccaac gaaaatttcc acttccgcca
    10681 gcgagtgtga ctcttcgcct ttcaaggctt cggctactgc cggagaagca tagaccagga
    10741 aacggtcgga gtcgtaagca tggtggacac gaacaatctc gcgcatgatt tcatagcata
    10801 ccgtttccac ggttttcacc gttccgcgac cgtggcaggt tgggcattcg ttacacagta
    10861 cgtgctcaat gctttcgcgg gtgcgtttac gcgtcatctc caccagcccc agcgccgaaa
    10921 aaccattaac gctggttttc acccgatctt tgctcaacgc ctgctccagc gagtgcagca
    10981 cgcggcgacg gtgatcttca ttattcatgt cgatgaaatc aataatgata atcccgccca
    11041 gattgcgcaa ccgtaactgg cgagcgatag cctgcgtcgc ttcaatattg gtattgaaaa
    11101 tggtgtcgtc cagattgcga tgaccgacaa acgccccggt attgatgtcc acggtggtca
    11161 tcgcttcggt ctggtcgata atcagataac caccggattt cagttctact ttgcgttcca
    11221 gcgctcgctg aatttcgttt tcgacatcaa agagatcgaa aatcggctgg cgtccggtgt
    11281 aatgctccag cttgctggtc atctcgggaa tgtactccga ggtaaactcc agcaatgctt
    11341 cgtaagtcag gcgtgagtca acgcgaatgc ggtcgagttc ggcgtcggcg aaatcacgca
    11401 gaacacgctg cgccagcgcc agttcgccgt acagctgata acgggtctgc gggcgttttt
    11461 tacgctccat aactttggtc cagacgcgtt tcagataagc ggcatcggag gccagttcag
    11521 cctcgccaac cccttccgct gcagtacgga tgataaaccc gccctgctcg tcgcaatact
    11581 ctgcgaccac ttttttcagg cgttcacgtt ctgattcgct ttcaatacgt tgggataccc
    11641 caacgtgaga agcccctggc ataaacacca gatagcgaga aggcagcgtg atatcggtgg
    11701 tcaggcgcgc acccttagtg ccaagcggat ctttcaccac ctgcaccatc agatcttgcc
    11761 cctgacggac cagttccgag atgtcgcgca ccgtgaattg cttttgttct tcacccgcca
    11821 cacattcggt gtgcggcatg atgtcagatg catgaagaaa cgcggcttta tccagcccaa
    11881 tatctacaaa agccgcctgc atacccggaa gtacacgact tacacgaccc ttgtagatat
    11941 tgcctactat tccgcgtcgc gcctcacgtt caatatgaat ttcctgcaga ataccgccat
    12001 caatatacgc cactcgcgtt tccgaaggcg ttacgtttac caacaattca gccgtcatgt
    12061 ttatcccttt tctcacgcag tgcgttaaaa ttacttaata attcatacgt ttcaaccagc
    12121 ggtaagccga ctacggcgtg aaagctgcca tttatcttcc tgacaaaaca gccacccagc
    12181 ccctgaatac cgtatgcacc tgctttatct aacggttcgc cgctggcgac atagcccgcg
    12241 atgtcttcgt ctgttaacgt tctgaaagtc acatcggtga ccaccaggca atcgagaatg
    12301 tgctggctgt cggccaacgc cactgccgtc atcacctgat gggtctgacc cgataatttg
    12361 cgcaacatct gcgccgcatg ctctgcgtcg cgcggtttct cgagcacttc tccgttcagg
    12421 ataacgatag tatccgcacc cagcaccggg agatcctgcg ccgtttgcgc gacacccgcc
    12481 tgtgctttct cgcgcgccag acgcacaaca tactgctgcg cgctctcctg cggctgacgc
    12541 tgctcctcaa tgcccgtaac aatacgttca aaggtcacgc caagttgcgc aagtaactcc
    12601 tgacgacgcg gagaaccgga agctaaatac agagaagtca tagaaacctt tattgcactg
    12661 caaactgctg acggacttta cgcatcagca agaaaatcca cggccagaga acgccattga
    12721 ctacactact ccagaacact tccggtctga aagagacgtt aatcactaaa aactctgccc
    12781 agaaaacaat aatatccacc accagcgaaa gcaacatgac gaccagcgcc tgctgccata
    12841 atgcgaggtt gcggaaaagc tggtatttca gcgccaccag gtaagcaatg atgctcatcg
    12901 ccaatacgcg tacgccaagc gtcgagccgc tgatcagatc cagtatggca cccatcacaa
    12961 aacctgtgcc cacatttacg cgatggggca aggccaggat ccaatacaac aagatgagta
    13021 acacccagtt tggccggaaa acaatcagat tatccggcca gggcatgatt tgcagcaaca
    13081 gcgcaatgag gaaagagagc cagattaccc agcgtccctg gctacgatag ctcgccacta
    13141 ttgtcctccc ggcgcacgcg caggcggttg agcaccactt tgcggcggcg tagccctttg
    13201 tggagagcga ttagctgcgg gctgtgtggg cgcagcaggc gcagtaactg catttcctgt
    13261 cgccggttgc tgcggagtcg gctgagtgat ccccgttgcc ggttcaggta actttggccc
    13321 catcgcgtct ggcgaaggca atacctgcgg catcatctgc atcagacgtt cattggcaac
    13381 acgatgcacc tcttccggcg tcatcgggtt agcgccgtta cgatccgcgc cccacagcag
    13441 cagcagataa cgcaaacgtt gcagtcctgc agtcggacgc gcctgaatca cagtataagc
    13501 gcgctgggta tcgagtttta cggaagagac aaccgcgacc ggatagcctt ccgggaaacg
    13561 accgcccaga ccggaagtca ccagcacatc accaacacga atatccgtat tcgctggcag
    13621 atgctcaagc tgcaaatcat ccgtacaacc gttaccggct gcaattacgc ggatatcgtt
    13681 gcgcagcacc tggattggca gcgcgtgggt cgcatcacaa atcagcagca cgcggctggt
    13741 cagtttagcg acggccacca cctgaccaac aacacctttg tcgctgatga ccggctggcc
    13801 ttcataaacg ccattaacgc tacctttatc gataacaact tgatcgctat aaggatcgtt
    13861 aaccgtggag ataacctgag tcaccatttt ctgctcatcc tgacgcagcg gggaacccag
    13921 cagctcgcgc agacgcgcgt tctcctgttt gtattgtcca agcatcagca gttcactgtt
    13981 tttcagcaac agttcctgac gtaacgcccg gttttcaagt tctagttggt cacgcgaggc
    14041 cagcgtctgc gatacgccat ccagcaattc acgaggagca ttggaaacaa agtagaaagg
    14101 actgacggcg gtatccatat aagtacggat ttgactgaac gtccccaggc ggctgtcggc
    14161 aataataatg ccgagcgcca ccagcaccgc cagaataagg cgaatctgta gcgacgggcc
    14221 acggctaaaa attggcttca taagttatgc gtattctcgt atcagaccag gcagggtaaa
    14281 cagacacttc ccctgcctgc atccgattac tcttcgctga acaggtcgcc gccgtgcatg
    14341 tcgatcattt ccagcgcttt gccgccaccg cgcgccacac aggtcagcgg gtcttcagca
    14401 acaacgactg gaatgccggt ttcttccatt aacaaacggt caaggttacg cagcagtgcg
    14461 ccaccaccgg tgagcaccat gccgcgctcg gagatatcgg aagccagttc cggcgggcac
    14521 tgttccagtg caaccattac cgcgctcaca ataccggtca gcggttcctg cagtgcttcg
    14581 aggatttcgt tggagttcag ggtaaaaccg cgtggaacac cttctgcaag gttacggcca
    14641 cgaacttcga tttcacggac ttcatcgccc gggtaagccg aaccgatttc gtgcttgata
    14701 cgttctgcgg tggcttcacc gatcagagaa ccgtaattac gacgtacata gttgatgata
    14761 gcttcgtcga aacggtcacc accaatgcgc acggaagagg agtaaaccac gccgttcaag
    14821 gagataacag caacttcagt ggtaccacca ccgatatcaa ccaccataga accggtcgct
    14881 tcagaaaccg gcaggccagc accaattgcg gcagccatcg gttcttcaat caggaagact
    14941 tcacgggcac cagcgccctg cgcggattca cgaattgcgc ggcgttcaac ctgggtcgcg
    15001 ccaaccggca cacaaaccag aacgcgcggg cttggacgca taaagctgtt gctgtgcact
    15061 tgtttgatga agtgctggag cattttttca gtcacgaaga agtcggcgat aacgccgtct
    15121 ttcattgggc gaatggcagc aatattgccc ggcgtacggc ccagcatctg cttcgcgtca
    15181 tgacctactg cagctacgct tttcggtgaa ccggcacgat cctgacgaat ggccaccacg
    15241 gaaggctcat tcaatacgat gccttgtcct tttacataaa tgagggtatt cgcagtaccc
    15301 aggtcaatgg acaagtcatt ggaaaacatg ccacgaaatt ttttcaacat actaagggat
    15361 aatcctgaaa gctggggcgg aaaagaaaat ccgcttactt taccaaccac acgcagcagc
    15421 gacaaggcgc aaaaatcatc tgctacggtg aaaattagtg cagttcgttt cctttgttac
    15481 aaatctctgc ctgagtccag aaaggcttaa tgcatcagca gcattcctcg cctgtttgca
    15541 accgcgtaag gtcattcatc tgcatatgtg ctgcaacaat ctggcgagca gacaagcaca
    15601 ctcccatgag acgcagcgcg cgttattcta cgtgaaaacg gattaaacgg caggttaaac
    15661 cgagtatctt tgtgaatatt ttttcacgtt agtatcaagt ggctgtgagg acgcgaaaaa
    15721 atccccttgc ccacctgtaa caccgcgctg aatcagggtc tgccactcgc ttcgcgaacg
    15781 tacgccggtg gcgtaaacct gggtgctggt cccggagcag gcttccacca ggctttgaac
    15841 cagcagctgg ttctccgttc gcttctcaat gtttctgacc agccccggat ggagcttgag
    15901 taactcaaca ttaagttctt tgatccaact ggtgcttacc agcgtcaaac cagcctggtt
    15961 gacggctacc cgtaccccta aagcattcac taaacgaata acaggttgta agcgactgat
    16021 atgttgacca acatcggcct ctgcaagttc aataattatg cgtcttcgtt gcgatttttc
    16081 acattgcatt aacgtatcgc gcagccaacg ctgaaaacgc gggcgaatca gcgactcaac
    16141 ggtaacctga atcgccagat tttcctctgg ccagtaacgc aatagtggaa taagacggct
    16201 gatttgcagg cggtcatact cttccgataa accaaactgc aagaccatcg gcatatactc
    16261 tgccgagcta acctcttcat taccatcgaa gatgcggcac atgagttcac gatgatgaac
    16321 ccggccttcg cgagtaaccg ccggtttttg ataaaggcgc gggccgccgc gactgagcat
    16381 ttgctcgata agcgtacgcc agcgaacatt accgcgtcct ttttcaggca acgagtcatc
    16441 gtaaatagcc cagctattgc cgccctgcaa tcccgcatta cgcgtggcag actctgcatg
    16501 ttccattacc tgctcggtat cctgaccact acgccaggca cagataccaa tgtggatcat
    16561 atcgtcgcga tcgagcattt tattgttcgg caaggtatcg acggctttga ttaactgact
    16621 ggcgatgctc tctgcctctt ttaacgtccg gtgcggtaac agcgcagcaa aatcactgcg
    16681 gtggtaacgc gccagcagtg cgccagggta gcgcatcata aatgtcgata gcagattcgt
    16741 cagagagaag aactgttctt caacctggct gtgcccccag gtatcactca acatattgaa
    16801 atccggcaga cgaatcatca tcacgatccc gtgggtacct actttctctt gatcttccag
    16861 taacgttgct aactgattat cgaaaaagag tcggttattg aggccggttt tcatgtcctg
    16921 ggcggcataa gagcggatca gcgtatcaag acggctgtgt tgttcgcgtg cgttctgaat
    16981 ttcacaaagc agcgtatcca gcgcactgct ggttctgggc ggccattcat agatggttcc
    17041 caacacatta gagccacgct caccgtttaa gatacgagta gcccgggttt ccagcaattc
    17101 ttgcccggca agttgccgtt gtaaccagcg taccgccagg aagagcataa gaatgataaa
    17161 gccaatcgcc cccgtgagcg gcgcggtagt cattaaagaa tggaaatagt tgcccatcgg
    17221 atcctgataa accagacgca gcgacatccc cggatgcttt atcaacggaa cgctcagttc
    17281 acgaaacaga tcgttggtgc caaccggacg ataactacca tttctggcca gggtataaac
    17341 ctgcttatca ccatggagca ggtctacacg aacgatatct gccgacatca ttaattcggt
    17401 aatttgtggc cttaatgtgc tgaagtcatt cgacacaagg taggtatcaa tcgccgtcgc
    17461 caccgcctga acacgatgac taaacttata ctgaatggcg ttgtagaaac ttagcgaaca
    17521 gcccagcaaa gtcacaaaaa ttgttaaccc ggtgagcagc gtgacaaagg ccgaaaattt
    17581 cgtcgttaat ctcatccttg tgttaactcc gatagtgagg aagcgggcat actagcaaat
    17641 cagatttatc tcgcaattta ttgcgcttca tcggctttgc ttttccatta gcgagtatag
    17701 tcttcagaaa ttattttcca atccatcatg cacatgagga ccacttatgc aggcgttact
    17761 tttagaacag caggacggca aaactctcgc atcagtacag actctggacg aaagtcgcct
    17821 gccggaaggc gatgtcacgg ttgatgttca ctggtcgagc ctgaactaca aagatgcgct
    17881 ggcgattacc ggtaagggaa aaatcatccg aaattttccg atgattcctg ggattgattt
    17941 tgccggaact gtacgcacca gcgaagatcc gcgttttcat gccggtcagg aggtgttact
    18001 cactggctgg ggcgttggtg aaaaccactg gggtgggctg gcggaacagg cgcgagtgaa
    18061 aggtgactgg ctggttgcca tgccgcaagg gctggacgcg cgtaaagcaa tgattatcgg
    18121 tactgccggt tttaccgcca tgctgtgtgt gatggcgctg gaagatgccg gtgttcgccc
    18181 gcaggatggg gaggttgtcg tgacgggtgc cagtggtggc gtcggcagta ccgccgtggc
    18241 gctgctgcat aagttgggtt atcaggtcgt tgccgtttcc ggtcgcgaaa gtacccatga
    18301 atatctgaaa agtttaggtg ccagccgtat tcttcctcgc gatgagtttg ctgaatcccg
    18361 tcctctggaa aaacaagtct gggctggggc aattgacacc gttggcgaca aagtgctggc
    18421 aaaagtgctg gcgcaaatga attacggcgg ctgcgtggcg gcctgtggtc tggcgggtgg
    18481 ttttactctg ccaaccacgg tcatgccatt tattctgcgt aatgtccgtt tgcaaggggt
    18541 ggattcagta atgacgccac cagcacgccg cgcacaagcc tggcagcgac tggtcgccga
    18601 tttaccggaa tcattctata cccaagcggc aaaagagata tctctggcag aggcaccgaa
    18661 gtttgccgag gccatcatta ataaccagat ccagggtcgc acgctggtga aggttaacta
    18721 accatttagc aaggattaat aagagaggga actcattttg aaatcattat tcaatcggtt
    18781 aacggaaaaa gcggttagtc ggacagcttt cgtcgaacac cttggtcagg aagttgtaca
    18841 acatcatcca aactggaaag tcatgatttc gactgaccat aaattgatgc gcattgatac
    18901 tccaccaaac agccattatt gatacgcctc cgtcgcctgt taggtttatg gtgctttgcc
    18961 ttggcgacgc tacgcttaat ctgttactga tttctggtac catgggggga ataatctgat
    19021 tttatttgat tacaaattaa ccacttaaac ctattgaatg cggaacattg tcagtcggtt
    19081 aattttgcgt gctttagcat tcacatcttt ccggacgatg cagtgaaaat tgggtaatcc
    19141 ccagcaaccg ctgcgtaatg tcgtctatct tgtcgcgatc ctggcatccc tacattattt
    19201 gtggtctgtg aagattatct cattgcagcc cctcatcttc gcagggctgg ctttacagct
    19261 tttcacctta cgttataaga agttccgtcg atgatggcgc taatttcgtg aattgtgcgg
    19321 cttgttgcaa attacacggt gttgaaggtt atttacatgt tagctgttga ttatcttccc
    19381 tgataagacc agtatttagc tgccaattgc tacgaaatcg ttataatgtg cgacctcgtc
    19441 ctccctgacg cagtttttgc gctgcggaaa aggtgacatt ggcgcaacga aggtatattt
    19501 tgttttttgc cggaggatag cagcagatcg ctgcacaatg tccgtcaagt ctaacattga
    19561 cactctgggg caaaatagac cggcgtcccg gcctgctgga atttatcgct atgcatacag
    19621 ctgtcggggc atacgcttta cagacggcgg tgaaacgcct gtcacaatca cactaaacaa
    19681 agagtacgga acccactcat ggatattcgt aagattaaaa aactgatcga gctggttgaa
    19741 gaatcaggca tctccgaact ggaaatttct gaaggcgaag agtcagtacg cattagccgt
    19801 gcagctcctg ccgcaagttt ccctgtgatg caacaagctt acgctgcacc aatgatgcag
    19861 cagccagctc aatctaacgc agccgctccg gcgaccgttc cttccatgga agcgccagca
    19921 gcagcggaaa tcagtggtca catcgtacgt tccccgatgg ttggtacttt ctaccgcacc
    19981 ccaagcccgg acgcaaaagc gttcatcgaa gtgggtcaga aagtcaacgt gggcgatacc
    20041 ctgtgcatcg ttgaagccat gaaaatgatg aaccagatcg aagcggacaa atccggtacc
    20101 gtgaaagcaa ttctggtcga aagtggacaa ccggtagaat ttgacgagcc gctggtcgtc
    20161 atcgagtaac gaggcgaaca tgctggataa aattgttatt gccaaccgcg gcgagattgc
    20221 attgcgtatt cttcgtgcct gtaaagaact gggcatcaag actgtcgctg tgcattccag
    20281 tgcggatcgc gatctaaaac acgtattact ggcagatgaa acggtctgta tcggccctgc
    20341 tccgtcagta aaaagttatc tgaacatccc ggcaatcatc agcgccgctg aaatcaccgg
    20401 cgcagtagca atccatccgg gttacggctt cctctccgag aacgccaatt ttgccgagca
    20461 ggttgaacgc tccggcttta tcttcatcgg cccgaaagca gaaaccattc gcctgatggg
    20521 cgacaaagta tccgcaatcg cggcgatgaa aaaagcaggc gttccttgcg taccgggttc
    20581 tgacggcccg ctgggcgacg atatggataa aaaccgcgcc attgctaaac gcattggtta
    20641 tccggtgatt atcaaagcct ccggcggcgg cggcggtcgc ggtatgcgcg tagtgcgcgg
    20701 cgacgctgaa ctggcacaat ccatctccat gacccgtgcg gaagcgaaag ctgctttcag
    20761 caacgatatg gtttacatgg agaaatacct ggaaaatcct cgccacgtcg agattcaggt
    20821 actggctgac ggtcagggca acgctatcta tctggcggaa cgtgactgct ccatgcagcg
    20881 ccgccaccag aaagtggtcg aagaagcacc ggctccgggc attaccccgg aactgcgtcg
    20941 ctacatcggc gaacgttgcg ctaaagcgtg tgttgatatc ggctatcgcg gtgcaggtac
    21001 tttcgagttc ctgttcgaaa acggcgagtt ctatttcatc gaaatgaaca cccgtattca
    21061 ggtagaacac ccggttactg aaatgatcac cggcgttgac ctgatcaaag aacagctgcg
    21121 tatcgctgcc ggtcaaccgc tgtcgatcaa gcaagaagaa gttcacgttc gcggccatgc
    21181 ggtggaatgc cgtatcaacg ccgaagatcc gaacaccttc ctgccaagtc cgggcaaaat
    21241 cacccgtttc cacgcacctg gtggttttgg cgtacgttgg gagtctcata tctacgcggg
    21301 ctacaccgta ccgccatact atgactcaat gatcggtaag ctgatttgct acggtgaaaa
    21361 ccgtgacgtg gcgattgctc gcatgaagaa tgcgctgcag gagctgatca tcgacggtat
    21421 caaaaccaac gttgatctgc agatccgcat catgaatgac gaaaacttcc agcatggtgg
    21481 cactaacatc cactatctgg agaaaaaact cggtcttcag gaaaaataat ttgtgacgcg
    21541 cggtgtttcc atcgcgctta gcaaagaacg taatcaacgc cgggcctgtt aatgctaccc
    21601 ggcgttttta ttgccgttaa ctaacggtag cctgcaattt ccgctttcaa cgcgctaatt
    21661 tcagcatcgc catcaacaat tcccagctca aagcagtatt tctttgtttc ccctggctca
    21721 ataaagggca gacgcccctg cgctcgttct ttatcacgac cgtcaggaaa actattactg
    21781 acctccagtc ccataacata ttcacccgcg cctaaatttt tccactggat aaagttgggt
    21841 agctgcatgg tgtcgaattt ctcgtaaatt ccccacccca gttcagcgtt caccagcgca
    21901 actttactca tcccctgact gtcgctattc aacgtgagat agtaaacctg ctcgtcaaaa
    21961 ccgggctgtg gggtacaaat ctccagatga ctggttaacc cttccgccgc atgcggggtt
    22021 cgtggcgtta cgcttttagc cgacagcaag atttctgcct gtggcgataa taaaggccag
    22081 ccgtaattaa agtgataaag aatttgcagc ggctggcggg tgaaaccatg attagtcacc
    22141 ttgtcttcaa tgcgcagcac attttcgcca taacggcagc gaatggtcct ttccagcgtc
    22201 agattttcac cgaacaatcg tgtttcccgt actttgccat tgatgacaaa ttcaatgccg
    22261 tcgtcggtcc gttctgtgcg ataccccacc tcttctgctg gagtcgcagc caaacgccca
    22321 tgtaacccca gcgtttctcc gttatcctca cagggcgtgc caatgtagct aagtccgcag
    22381 gtagtcagta accccgcgta gaagctacgt aaaaacccgt tgccctgaga ctcaaaaaaa
    22441 gcgggggcga caatgccgtt gggggtgata aaagagagcg cagtgtcctt gtagctggcc
    22501 catgcaatat ccagcgcgcg atcttttacc accgtaaatg ccagtcccgc accagtacgg
    22561 acatcaaacg cttctgtccc ctttgctctg ccactacgta actcatagtg tttaattccg
    22621 gcaacctgcg agagatcgcc aatccacgac caaatttcgt ttttcattgt gctgcttccc
    22681 gaatggacat gtcatggcgc tggatgaacg tttcgatttc cataaggcga gacgttccct
    22741 ccattgggcc acgtcgggtt accgccagtg caccgcaggc gttggcatat tgtaatgccc
    22801 gatgggcgtc aaaccctagc tggcggcagg caatccacgc gccaccaaag cagtctcccg
    22861 caccggtcgg gtcaacttct tccaccggat agctctcaac atgaaactgc tcattcgcgg
    22921 agtagtaact ggcaccttga ttgccgcgct tcacaatcac ctctttaaca ccttcttcaa
    22981 gaaaaccagc gattgcacgc tccggcgtcg agtgtggcga caagagcaac acttctccct
    23041 cgctgggcat atagatatct gttaactcaa gaacaaagtg gagggcgtca cgcatttctg
    23101 gaatatcaag catttcttta cgaatattcg ggtcgaaaga aataacgcca ccatttgctt
    23161 taacgatggt tactgctttt tttactgcgt ctaccatatg gaatgaaaac agggatgaac
    23221 ccataatatg aaaatgggta caatccttca gaatattttc atcaacatgc tgtgccgata
    23281 atttaccgca agcggcattc ttgatattaa aaataaaatc gcgatcgcca gagttatgat
    23341 aagtaacaaa ggcactaccg gtggcttcca gaggtaagac tgaaatcccc ctgatgtcca
    23401 caccgtcagc agccagacga tgtatattaa tatcaccaaa gccatcattc cccacgcagc
    23461 taatgatgcc gcacgggacg ccaagacgtg tcacctggtc gataaaaata gcgggcgcac
    23521 cactgggata agggccgttc cagatacctg gttgatcaaa tggctggccg attttactcg
    23581 ccataatttc aaccagaatt tctccaattg tgaagacctt actcatatga ttaattcctt
    23641 gaaatgatta gctgcgtttt cttttatttc ttacgtcaaa ataaacggct acaagaatga
    23701 ctaatccttt aaccaccatc tggtaaaaat aaggaacatt cattaagttc atgccattat
    23761 taataacgcc gatggttaat gcgccgatca ccgtccccac cacggttcca tagccgccgt
    23821 tcaggctggt gcctcccagt acagctgcgg caatagcatc cagttcgtag ctcaacgcgg
    23881 aattcggttg cccggaatag aggcgtgagg caaggatcat cccgctgata cctgccagca
    23941 cggctgtaat cataaagact ttaattttca agcgggtgac atttatccct gaataaattg
    24001 ctgcttcttt attaccacca gtgatataaa tttttcgtcc aaaggttgtt ttagtaagaa
    24061 tgaagtggtt tatcagcaat aagatgatta atatccatat aggaataggt atatagagaa
    24121 attctccatt ccccagcgcc agaaaggcat catctttaat catgatgggc ataccatcgg
    24181 tcactatata agcgatacca cggaagatcc ccatagtagc aacggtgaca ataaacgacg
    24241 ggatccccgc tatggccgtc aagccaccat ttatcatccc cagagccagt gctgcaataa
    24301 tggataatgg gaccaataaa ataaatggaa ttccccatga agccccaagc gcagcaaatg
    24361 tgccaatcaa tgcaatggtt gaaccaaccg ataaatcaat atcgccaagc aaaagtacgt
    24421 aagtcattcc ataggcggta attgcaatta tcgaaacctg aagaataata ttagtaatgt
    24481 tattggcggt aaaaaaactg tcattaataa aactgaacag aatgattaac accaccattc
    24541 cactgaggat accaccatat tgcctgaaga cttttttaaa attatttgca atgggaattt
    24601 ccgaaggttt cggactggca agagttttct ctgtcattta acgtactcct gttgcagcgg
    24661 tcattacctg tgtctgcgtt aaattttcac atgaataaat atcggcaatc gttttattac
    24721 gcattaccac cagacgatcg ctaagtgcca tgacttctgg cagttcagag gacaccagaa
    24781 taattgctgt ccctgccgca gccagttgac gtatcacgtt atagatttca aattttgcgc
    24841 cgacatccac tccgcgcgtt ggctcatcga gcaataaaat acgcggagtt ttctccagcc
    24901 acttcgataa cacgattttc tgttgattac cgccgctcag tgctcccact gcctgatgca
    24961 cagaactcac tttagtgttc attctggcaa ttgattgcag cgtcttcgcc tcttcttgtc
    25021 ggtgattgat gagtcccatc cgcgaagcgt tatattgaaa cgccacatgg tgcgtgttca
    25081 tattgacgtc gtgcatcagc actaaccctt cttccttgcg gttttcggtc acaaaagcaa
    25141 tgccatgatc aatagcctgt ttgggattag cgatcctgac ttccttacca tcaatcaaca
    25201 cagtcccgtg atacgaaacg aggccaaata gcgccttcat cacatcggaa cgcccggccc
    25261 ccaccaggcc aaaaaatccg aggacttccc ccggacgcac agcgaaactg acctctttaa
    25321 aaagcgaagg atgggaaaga ttttttactt ccagttttgc aggaacattt tcatccggtt
    25381 tttccccaag acgtgctggc caaatatttt tcatttcccg accaaccatt tgggcaatca
    25441 agtgctggat atcactgttt ttcttttcat gcgtagcgat atactcaccg tcgcggatca
    25501 ccgaaatatc atctgaaatc cgcataattt catccatgcg atgagagata taaataaccg
    25561 cacagcctcg ctgctttaat ttttcaataa tgttaaacag gatctccgct tcattatcac
    25621 ttagcgaaga tgtcggttca tccataatca cgacatccgc tgcatagctt aaggctttag
    25681 caatttctac cagttgccta tgtgccatcg acatattgcc gaccatttcc agagggtcta
    25741 gtgggatgcc cagttccgcg aggaggttct ggcaatctgc gagcattttt ttatcgttaa
    25801 tgatgccaaa acgtctcggt tcattattgg cataaatatt ttccgccacg gtcatattat
    25861 tactcaggct cagttcctga aaaataatcg caatccctaa ctggcgagaa tgctgcggat
    25921 tattaatggt gatggtttca ccacgtagtg ttatcgttcc ctgggttggc ggataaacac
    25981 cggccagtat tttcattaat gtcgatttac cggcaccatt ctcgcctaat aaagtatgca
    26041 cccgcccttt acgtaaagag agtgtaatat cttttaatgc ctgaacatga ccaaatgtct
    26101 tcgcaatatg cgaaacatca agaataacat ccgtcataat gacctcggtg tcacaacaaa
    26161 acgccaataa cgttttgttg tgaggtgaag atatttacta tttacctgta taaacgccgg
    26221 gttgtaccgg gatcattttt tctaccggtt taccattgag gtgatcaaca gcggcatcaa
    26281 tggctttagc acccatttga tcgggatatt gccgaatcac ggccgcaaac gttttttcac
    26341 tgtcaacggc atcacgcgct tctttcatgc cgtcaaaacc aatgattttg atgttgtcgt
    26401 tatgcgcaga ttttgctgcg ataaccgctg ccagcgccgc atcatcacca aagccaaaca
    26461 gaccattcag gttagcattt gcctgcataa tgttttgcgc agtcgttaac gcttcagccc
    26521 gcgtaatacc aggctgttca gcaacaattt taatatccgg tgtatcgctt aaccctttct
    26581 taaaaccatc aacgcgcgca accaccgatt gtagtgccgg ataagaaata atggctacag
    26641 tacctttacc atgcaaaagt ttacccatta acttgcctgc ttcaacgcct cccgcatagt
    26701 tatcggtcgc aacatgcgat acgaccggaa cgccttccgc agcgacgtca acggtgataa
    26761 cgggaatacc ggccttatcg gctttaatta tcgctgcctg gacgcctttc gaatcgaccg
    26821 gagagataat aatggcatcc acttttttag taataaaatc ctcaacatca gaaagttgtt
    26881 tgtttaaatc ctgattggca atagagacac ttaaattgac attgtctttt tttgcctctt
    26941 ctttcatggc attcgcaaga tcaatataga aaggatgttg ttgtgttaat aatgacgcac
    27001 caattttata ctcttttgcc aacgaacttt gcgagaacaa tgccaggctc accaacatag
    27061 ctgaaccaag taatttaata tttttcataa acaatcctca ttatttttgt agggtacaac
    27121 gtcttaaatt tttccttcac taccacagac gccaataata cgagttacga cttctttcat
    27181 ggcggccttt gcgggctgca tatagtgacg tggatcattt gccgcaggat tgttgtagaa
    27241 atatcctttt aacgcatcgg caaacgcaat tttcaattct gtcgcgacat tcactttaca
    27301 aataccgcga cgaatacagt gctgaacgtc ctgttccgat aagcccgaag caccatgcaa
    27361 caccagcgga atctccactc gctgacgaat ctgttccagc cgcacaaaat ccagtcgtgg
    27421 ttcatgcgca tataaaccat gtgctgtgcc gattgctacc gccagggagt caatttgggt
    27481 acgttgaaca aactctgcag ctgcggctgg tgaggtataa gcggaatctt tttcatccac
    27541 ccgcagatcg tcctcctggc cgccaagacg ccctagctcg gcctcaacgc taacatcaaa
    27601 gcgatggcaa aaggtggtaa cttcttgcac tcgcgcaatg ttgtcttcaa atggcaaatg
    27661 ggagccatca atcatgacag aacggatccc tgcgcgaacc ttgttaaaaa tgtcatcact
    27721 ctcttcatga tggtcgagat gcaggactaa cggcaggttg tactcgcgag ccagcgattg
    27781 tactaacgaa attaaacttc ccacgccacc ataacgatat gtaccgggtg ttcccgccag
    27841 cataacggga gagcgaagtt gtgcagcgct ctcaacaact acctgtaaag tttcaaggtt
    27901 atgaatgttg aaagcaggga cggcatatcc ctctgactgg gcttttttca gcatgttttt
    27961 gcttgaaata atatacatcg cagctcctgt tattggtcgt tcgccttaca aaatgaagaa
    28021 agagaatctt tactttcatt ttgaaagcaa actagcacag caaa
//
LOCUS       DADSYM010000057        28043 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2415
            SAMN08039579-rid11723173.denovo.057, whole genome shotgun sequence.
ACCESSION   DADSYM010000057 DADSYM010000000
VERSION     DADSYM010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039579
            Sequence Read Archive: SRR6362533
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 28043)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28043)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980162.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 03:39:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,484
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 4,347
            CDSs (with protein)               :: 4,347
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 43 of 85
            Pseudo Genes (incomplete)         :: 37 of 85
            Pseudo Genes (internal stop)      :: 19 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28043
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039579-rid11723173.denovo.057"
                     /strain="LGE2415"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8302 N 95.9628 W"
                     /collection_date="2015-08-24"
     gene            40..114
                     /locus_tag="J1Y83_002025"
     tRNA            40..114
                     /locus_tag="J1Y83_002025"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:72..74,aa:Gln,seq:ctg)
     gene            complement(268..1443)
                     /gene="ubiF"
                     /locus_tag="J1Y83_002026"
     CDS             complement(268..1443)
                     /gene="ubiF"
                     /locus_tag="J1Y83_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-octaprenyl-3-methyl-6-methoxy-1,4-benzoquinol
                     hydroxylase"
                     /protein_id="HBA7630173.1"
                     /translation="MTNQPTEIAIVGGGMVGGALALGLAQHGFTVTVIEHAQPAPFVA
                     DSQPDVRISAISAASVSLLKGVGVWDAVQAMRCHPYRRLETWEWETAHVVFDAAELKL
                     PLLGYMVENTVLQQALWQALEAHPKVTLRVPTSLIALHRHNDLQELELKGGETILAKL
                     VIGADGANSQVRQMAGIGVHAWQYAQSCMLISVQCENDPGDSTWQQFTPDGPRAFLPL
                     FDNWASLVWYDSPARIRQLQNMNMAQLQAEIVKHFPSRLGYVTPLAAGAFPLIRRHAL
                     QYVQPGLALVGDAAHTIHPLAGQGVNLGYRDVDALIDVLVNARSYGEAWASYPVLKRY
                     QMRRMADNFMMQSGMDLFYAGFSNNLPPLRFVRNLGLMAAERAGVLKRQALKYALGL"
     gene            1589..3013
                     /gene="miaB"
                     /locus_tag="J1Y83_002027"
     CDS             1589..3013
                     /gene="miaB"
                     /locus_tag="J1Y83_002027"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HBA7630174.1"
                     /translation="MTKKLHIKTWGCQMNEYDSSKMADLLDATHGYQLTDVAEEADVL
                     LLNTCSIREKAQEKVFHQLGRWKLLKEKNPDLIIGVGGCVASQEGEHIRQRAHYVDII
                     FGPQTLHRLPEMINSVRGDRSPVVDISFPEIEKFDRLPEPRAEGPTAFVSIMEGCNKY
                     CTYCVVPYTRGEEVSRPSDDILFEIAQLAAQGVREVNLLGQNVNAWRGENYDGTTGTF
                     ADLLRLVAAIDGIDRIRFTTSHPIEFTDDIIEVYRDTPELVSFLHLPVQSGSDRILNL
                     MGRTHTALEYKAIIRKLRAARPDIQISSDFIVGFPGETTEDFEKTMKLIADVNFDMSY
                     SFIFSARPGTPAADMVDDVPEEEKKQRLYILQERINQQAMAWSRRMLGTTQRILVEGT
                     SRKSIMELSGRTENNRVVNFEGTPDMIGKFVDVEITDVYPNSLRGKVVRTEDEMGLRV
                     AETPESVIARTRKENDLGVGYYQP"
     gene            3127..4206
                     /locus_tag="J1Y83_002028"
     CDS             3127..4206
                     /locus_tag="J1Y83_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706552.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HBA7630175.1"
                     /translation="MFSFRRHTDKRNGLNIDTREITLEPADNARLLSLCGPFDDNIKQ
                     LERRLGIEINRRDNHFKLTGRPICVTAAADILRSLYVDTAPMRGQIQDIEPEQIHLAI
                     KEARVLEQSAESVPEYGKAVNIKTKRGVIKPRTPNQAQYIANILDHDITFGVGPAGTG
                     KTYLAVAAAVDALERQEIRRILLTRPAVEAGEKLGFLPGDLSQKVDPYLRPLYDALFE
                     MLGFEKVEKLIERNVIEVAPLAYMRGRTLNDAFIILDESQNTTIEQMKMFLTRIGFNS
                     KAVITGDVTQIDLPRNTKSGLRHAIEVLADVEEISFNFFHSEDVVRHPVVARIVNAYE
                     AWEEAEQKRKAALAAERKREEQEQK"
     gene            4203..4670
                     /gene="ybeY"
                     /locus_tag="J1Y83_002029"
     CDS             4203..4670
                     /gene="ybeY"
                     /locus_tag="J1Y83_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014169133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HBA7630176.1"
                     /translation="MSQVILDLQLACEDNSGLPEESQFQTWLNAVIPQFQEESEVTIR
                     VVDTAESHSLNLTYRGKDKPTNVLSFPFEVPPGMEMSLLGDLVICRQVVEKEAQEQGK
                     PLEAHWAHMVVHGSLHLLGYDHIEDDEAEEMEALETEIMLALGYEDPYIAEKE"
     gene            4760..5638
                     /gene="corC"
                     /locus_tag="J1Y83_002030"
     CDS             4760..5638
                     /gene="corC"
                     /locus_tag="J1Y83_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308723.1"
                     /note="CorC(YbeX) belongs to the Cyclin M Mg2+ Exporter
                     (CNNM) family, and was characterized as belonging to a set
                     of three proteins, at least one of which must be present
                     for CorA to function; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM family magnesium/cobalt transport protein
                     CorC"
                     /protein_id="HBA7630177.1"
                     /translation="MSDDNSHSSDTISNKKGFFSLLLSQLFHGEPKNRDELLALIRDS
                     GQNDLIDEDTRDMLEGVMDIADQRVRDIMIPRSQMITLKRNQTLDECLDVIIESAHSR
                     FPVISEDKDHIEGILMAKDLLPFMRSDAEAFSMDKVLRQAVVVPESKRVDRMLKEFRS
                     QRYHMAIVIDEFGGVSGLVTIEDILELIVGEIEDEYDEEDDIDFRQLSRHTWTVRALA
                     SIEDFNEAFGTHFSDEEVDTIGGLVMQAFGHLPARGETIDIDGYQFKVAMADSRRIIQ
                     VHVKIPDDSPQPKLDE"
     gene            5663..7204
                     /gene="lnt"
                     /locus_tag="J1Y83_002031"
     CDS             5663..7204
                     /gene="lnt"
                     /locus_tag="J1Y83_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HBA7630178.1"
                     /translation="MAFASLIERQRIRLLLALLFGACGTLAFSPYDVWPAAIISLMGL
                     QALTFNRRPLQSAAIGFCWGFGLFGSGINWVYVSIATFGGMPGPVNIFLVVLLAAYLS
                     LYTGLFAGVLSRLWPKTTWLRVAIAAPALWQVTEFLRGWVLTGFPWLQFGYSQIDGPL
                     KGLAPIMGVEAINFLLMMVSGLLALALVKRNWRPLVVAVVLFALPFPLRYIQWFTPQP
                     EKTIQVSMVQGDIPQSLKWDGDQLLNTLKIYYNATAPLMGKSSLIIWPESAITDLEIN
                     QQPFLKALDGELRDKGSSLVTGIVDARLNKQNRYDTYNTIITLGKGAPYSYESADRYN
                     KNHLVPFGEFVPLESILRPLAPFFDLPMSSFSRGPYIQPPLSLNGIQLTAAICYEIIL
                     GEQVRDNFRPDTDYLLTISNDAWFGKSIGPWQHFQMARMRALELARPLLRSTNNGITA
                     VIGPQGEIQAMIPQFTREVLTTNVTPTTGLTPYARTGNWPLWVLTALFGFAAVLMSLR
                     QRRKR"
     gene            complement(7336..9177)
                     /locus_tag="J1Y83_002032"
     CDS             complement(7336..9177)
                     /locus_tag="J1Y83_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001276742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HBA7630179.1"
                     /translation="MSASSVKPLNVQLPAITLILFALCVGIFCYLAQWMSYEEVDQSA
                     LIHLGANVASLTLSDESWRLLSSVFLHSSFSHLLMNMFALLAVGTVAERILGKWRLLI
                     IWLFSGIFGGLISACYALRESEQIVISVGASGAIMGIAGTHHKNQRRVFPLLGMVALT
                     LLYGTRQTGIDNACHIGGLIAGGALGWLSARLVGQNRLVTEGGIIVAVTLLLTGTIWF
                     VQQQIDESVLQVRQSLREAFYPQEIEQERRQKKQQLVEERNALRETLSAPVSREQASG
                     DLLAEIADIHDMAISRDGNTLYAAIENTNSIVVFDLGQKKILHTFTAPIAKEKSVKHC
                     GGCKDQGVRSLALSLDEKLIYATSFEANAVSVINVATGEIIQSITTGAHPDSLILSRD
                     GTKAWVMNRTSNSVSAIDLVTYQHVADIPLEKYDGTGTSGKPGAWVMALSPDEKTLLV
                     PGAGRGNIVRINTITHQKEDFPAGDARGVVSAMGFRPKNGEIIFADSQGISRIRAEDQ
                     QASIMTQWCSRSVYSVEGISPDGQYLALVSYGLQGYVILLNINAGQIIGVYPASYVNN
                     LRFSADGRKIFVMAKNGLIQLDRESSLDPQAIIRHPQYGDVACIPEP"
     gene            9631..10539
                     /gene="gltI"
                     /locus_tag="J1Y83_002033"
     CDS             9631..10539
                     /gene="gltI"
                     /locus_tag="J1Y83_002033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter
                     substrate-binding protein GltI"
                     /protein_id="HBA7630180.1"
                     /translation="MQLRKPATAILALALSAGLAQADDAAPAAGSTLDKIAKNGVIVV
                     GHRESSVPFSYYDNQQKVVGYSQDYSNAIVEAVKKKLNKPDLQVKLIPITSQNRIPLL
                     QNGTFDFECGSTTNNAERQKQAAFSDTIFVVGTRLLTKKGGDIKDFADLKGKAVVVTS
                     GTTSEVLLNKLNEEQKMNMRIISAKDHGDSFRTLESGRAVAFMMDDALLAGERAKAKK
                     PDNWEIVGKPQSQEAYGCMLRKDDPQFKKLMDDTIAQVQTSGEAEKWFDKWFKNPIPP
                     KNLNMNFELSDEMKALFKEPNDKALN"
     gene            10709..11449
                     /gene="gltJ"
                     /locus_tag="J1Y83_002034"
     CDS             10709..11449
                     /gene="gltJ"
                     /locus_tag="J1Y83_002034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter permease
                     GltJ"
                     /protein_id="HBA7630181.1"
                     /translation="MSIDWNWGIFLQQAPFGNTTYLGWIWSGFQVTIALSICAWIIAF
                     LVGSFFGILRTVPNRFLSGLGTLYVELFRNVPLIVQFFTWYLVIPELLPEKIGMWFKA
                     ELDPNIQFFLSSMLCLGLFTAARVCEQVRAAIQSLPRGQKNAALAMGLTLPQAYRYVL
                     LPNAYRVIVPPMTSEMMNLVKNSAIASTIGLVDMAAQAGKLLDYSAHAWESFTAITLA
                     YVLINAFIMLVMTLVERKVRLPSNMGGK"
     gene            11449..12123
                     /gene="gltK"
                     /locus_tag="J1Y83_002035"
     CDS             11449..12123
                     /gene="gltK"
                     /locus_tag="J1Y83_002035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter permease
                     GltK"
                     /protein_id="HBA7630182.1"
                     /translation="MYEFDWSSIVPSLPYLLDGLVITLKITVTAVVIGILWGTMLAVM
                     RLSSFAPVAWFAKAYVNVFRSIPLVMVLLWFYLIVPGFLQNVLGLSPKNDIRLISAMV
                     AFSMFEAAYYSEIIRAGIQSISRGQSSAALALGMTHWQSMKLIILPQAFRAMVPLLLT
                     QGIVLFQDTSLVYVLSLADFFRTASTIGERDGTQVEMILFAGFVYFVISLSASLLVSY
                     LKRRTA"
     gene            12123..12848
                     /gene="gltL"
                     /locus_tag="J1Y83_002036"
     CDS             12123..12848
                     /gene="gltL"
                     /locus_tag="J1Y83_002036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020457015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter ATP binding
                     protein GltL"
                     /protein_id="HBA7630183.1"
                     /translation="MITLKNVSKWYGHFQVLTDCSTEVKKGEVVVVCGPSGSGKSTLI
                     KTVNGLEPVQQGEITVDGIVVNDKKTDLAKLRSRVGMVFQHFELFPHLSIIENLTLAQ
                     VKVLKRNKAPAREKALKLLERVGLSAHANKFPAQLSGGQQQRVAIARALCMDPIAMLF
                     DEPTSALDPEMINEVLDVMVELANEGMTMMVVTHEMGFARKVANRVIFMDEGKIVEDS
                     PKDAFFDDPKSDRAKDFLAKILH"
     gene            12966..13901
                     /gene="rihA"
                     /locus_tag="J1Y83_002037"
     CDS             12966..13901
                     /gene="rihA"
                     /locus_tag="J1Y83_002037"
                     /EC_number="3.2.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-specific ribonucleoside hydrolase
                     RihA"
                     /protein_id="HBA7630184.1"
                     /translation="MALPILLDCDPGHDDAIAIVLALASPELDVKAITSSAGNQTPEK
                     TLRNVLRMLTLLNRTDIPVASGAVKPLMRNLIIADNVHGESGLDGPALPEPTFAPQNC
                     TAVELMAKTLCESEEPVTIVSTGPQTNVALLLNSHPELHSKIARIVIMGGAMGLGNWT
                     PAAEFNIYVDPEAAEIVFQSGIPVVMAGLDVTHKAQIHVEDTERFRAIGNPVSTIVAE
                     LLDFFLEYHKDEKWGFVGAPLHDPCTIAWLLKPELFTTVERWVGVETQGKYTQGMTVV
                     DYYYLTGNKPNATVMVDVDRQGFVDLLADRLKFYA"
     gene            complement(13946..14428)
                     /locus_tag="J1Y83_002038"
     CDS             complement(13946..14428)
                     /locus_tag="J1Y83_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon-containing protein"
                     /protein_id="HBA7630185.1"
                     /translation="MNKVAQYYRELVASLSERLRNGERDIDALVEQARERVIKTGELT
                     RTEVDELTRAVRRDLEEFAMSYEESLKEESDSVFMRVIKESLWQELADITDKTQLEWR
                     EVFQDLNHHGVYHSGEVVGLGNLVCEKCHFHLPIYTPEVLTLCPKCGHDQFQRRPFEP
                     "
     gene            14663..17245
                     /gene="leuS"
                     /locus_tag="J1Y83_002039"
     CDS             14663..17245
                     /gene="leuS"
                     /locus_tag="J1Y83_002039"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008456064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HBA7630186.1"
                     /translation="MQEQYRPEEIESKVQLHWDEKRTFEVTEDESKEKYYCLSMLPYP
                     SGRLHMGHVRNYTIGDVIARYQRMLGKNVLQPIGWDAFGLPAEGAAVKNNTAPAPWTY
                     DNIAYMKNQLKMLGFGYDWSRELATCTPEYYRWEQKFFTELYKKGLVYKKTSAVNWCP
                     NDQTVLANEQVIDGCCWRCDTKVERKEIPQWFIKITAYADELLNDLDKLDHWPDTVKT
                     MQRNWIGRSEGVEITFNVNDYDNTLTVYTTRPDTFMGCTYLAVAAGHPLAQKAAENNP
                     ELAAFIDECRNTKVAEAEMATMEKKGVDTGFKAVHPLTGEEIPVWAANFVLMEYGTGA
                     VMAVPGHDQRDYEFASKYGLNIKPVILAADGSEPDLSQQALTEKGVLFNSGEFNGLDH
                     EAAFNAIADKLTAMGVGERKVNYRLRDWGVSRQRYWGAPIPMVTLEDGTVMPTPDDQL
                     PVILPEDVVMDGITSPIKADPEWAKTTVNGMPALRETDTFDTFMESSWYYARYTCPEY
                     KEGMLDSKAANYWLPVDIYIGGIEHAIMHLLYFRFFHKLMRDAGMVNSDEPAKQLLCQ
                     GMVLADAFYYVGENGERNWVSPVDAIVERDEKGRIVKAKDAAGHELVYTGMSKMSKSK
                     NNGIDPQVMVERYGADTVRLFMMFASPADMTLEWQESGVEGANRFLKRVWKLVYEHTA
                     KGDVAALNVDALTEDQKALRRDVHKTIAKVTDDIGRRQTFNTAIAAIMELMNKLAKAP
                     TDGEQDRALMQEALLAVVRMLNPFTPHICFTLWQELKGEGDIDNAPWPVADEKAMVED
                     STLVVVQVNGKVRAKITVPVDATEEQVRERAGQEHLVAKYLDGVTVRKVIYVPGKLLN
                     LVVG"
     gene            17260..17841
                     /gene="lptE"
                     /locus_tag="J1Y83_002040"
     CDS             17260..17841
                     /gene="lptE"
                     /locus_tag="J1Y83_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly lipoprotein LptE"
                     /protein_id="HBA7630187.1"
                     /translation="MRYLATLLLSLAVLITAGCGWHLRDTTQVPSTMKVMILDSGDPN
                     GPLSRAVRNQLRLNGVELLDKETTRKDVPSLRLGKVSIAKDTASVFRNGQTAEYQMIM
                     TVNATVLIPGRDIYPISAKVFRSFFDNPQMALAKDNEQDMIVKEMYDRAAEQLIRKLP
                     SIRAADIRSDEEQTSTTTDTPATPARVSTTLGN"
     gene            17841..18872
                     /gene="holA"
                     /locus_tag="J1Y83_002041"
     CDS             17841..18872
                     /gene="holA"
                     /locus_tag="J1Y83_002041"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HBA7630188.1"
                     /translation="MIRLYPEQLRAQLNEGLRAAYLLLGNDPLLLQESQDAVRQVAAA
                     QGFEEHHTFSIDPNTDWNAIFSLCQAMSLFASRQTLLLLLPENGPNAAINEQLLTLTG
                     LLHDDLLLIVRGNKLSKAQENAAWFTALANRSVQVTCQTPEQAQLPRWVAARAKQLNL
                     ELDDAANQVLCYCYEGNLLALAQALERLSLLWPDGKLTLPRVEQAVNDAAHFTPFHWV
                     DALLMGKSKRALHILQQLRLEGSEPVILLRTLQRELLLLVNLKRQSAHTPLRALFDKH
                     RVWQNRRGMMSEALNRLSQPQLRQAVQLLTRTELTLKQDYGQSVWAELEGLSLLLCHK
                     PLADVFIDG"
     gene            18874..19515
                     /gene="nadD"
                     /locus_tag="J1Y83_002042"
     CDS             18874..19515
                     /gene="nadD"
                     /locus_tag="J1Y83_002042"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HBA7630189.1"
                     /translation="MKSLQALFGGTFDPVHYGHLKPVETLANLIGLTRVTIIPNNVPP
                     HRPQPEANSVQRKHMLELAIADKPLFTLDERELKRNAPSYTAQTLKEWRQEQGPDVPL
                     AFIIGQDSLLTFPTWYEYETILDNAHLIVCRRPGYPLEMAQPQYQQWLEDHLTHNPED
                     LHLQPAGKIYLAETPWFNISATIIRERLQNGESCEDLLPEPVLTYINQQGLYR"
     gene            19539..20150
                     /locus_tag="J1Y83_002043"
     CDS             19539..20150
                     /locus_tag="J1Y83_002043"
                     /EC_number="3.1.3.73"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobalamin/alpha-ribazole phosphatase"
                     /protein_id="HBA7630190.1"
                     /translation="MRLWLIRHGETQANVDGLYSGHAPTPLTARGIEQAQNLHTLLHD
                     VSFDLVLCSELERAQHTARLVLSDRQHPVHIIPELNEMFFGDWEMRHHRDLMQEDAEN
                     YSAWCNDWQHAIPTNGEGFQAFSQRVERFIARLSEYQHYQNILVVSHQGVLSLLIARL
                     IGMPAESMWHFRVDQGCWSAIDINQKFATLRVLNSRAIGVENA"
     gene            20410..20727
                     /gene="rsfS"
                     /locus_tag="J1Y83_002044"
                     /gene_synonym="rsfA"
     CDS             20410..20727
                     /gene="rsfS"
                     /locus_tag="J1Y83_002044"
                     /gene_synonym="rsfA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HBA7630191.1"
                     /translation="MQGKALQDFVIDKIDDLKGQDIIALDVQGKSSITDCMIICTGTS
                     SRHVMSIADHVVQESRAAGLLPLGVEGENSADWIVVDLGDVIVHVMQEESRRLYELEK
                     LWS"
     gene            20731..21198
                     /gene="rlmH"
                     /locus_tag="J1Y83_002045"
     CDS             20731..21198
                     /gene="rlmH"
                     /locus_tag="J1Y83_002045"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010618862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HBA7630192.1"
                     /translation="MKLQLVAVGTKMPDWVQTGFTEYLRRFPKDMPFELIEIPAGKRG
                     KNADIKRILDKEGEQMLAAAGKNRIVTLDIPGKPWDTPQLAAELERWKLDGRDVSLLI
                     GGPEGLSPACKAAAEQSWSLSALTLPHPLVRVLVAESLYRAWSITTNHPYHRE"
     gene            21229..23130
                     /gene="mrdA"
                     /locus_tag="J1Y83_002046"
     CDS             21229..23130
                     /gene="mrdA"
                     /locus_tag="J1Y83_002046"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-transpeptidase MrdA"
                     /protein_id="HBA7630193.1"
                     /translation="MKLQNSFRDYTAESALFVRRALVAFLGILLLTGVLIANLYNLQI
                     VRFTDYQTRSNENRIKLVPIAPSRGIIYDRNGIPLALNRTIYQIEMMPEKVDNVQQTL
                     DALRSVVDLTDDDIAAFRKERARSHRFTSIPVKTNLTEVQVARFAVNQYRFPGVEVKG
                     YKRRYYPYGSALTHVIGYVSKINDKDVERLNNDGKLANYAATHDIGKLGIERYYEDVL
                     HGQTGYEEVEVNNRGRVIRQLKEVPPQAGHDIYLTLDLKLQQYIETLLAGSRAAVVVT
                     DPRTGGVLALVSTPSYDPNLFVDGISSKDYSALLNDPNTPLVNRATQGVYPPASTVKP
                     YVAVSALSAGVITRNTTLFDPGWWQLPGSEKRYRDWKKWGHGRLNVTRSLEESADTFF
                     YQVAYDMGIDRLSEWMGKFGYGHYTGIDLAEERSGNMPTREWKQKRFKKPWYQGDTIP
                     VGIGQGYWTATPIQMSKALMILINDGIVKVPHLLMSTAEDGKQVPWVQPHEPPVGDIH
                     SGYWELAKDGMYGVANRPNGTAHKYFASAPYKIAAKSGTAQVFGLKANETYNAHKIAE
                     RLRDHKLMTAFAPYNNPQVAVAMILENGGAGPAVGTLMRQILDHIMLGDNNTDLPAEN
                     PAVAAAEDH"
     gene            23133..24245
                     /gene="mrdB"
                     /locus_tag="J1Y83_002047"
                     /gene_synonym="rodA"
     CDS             23133..24245
                     /gene="mrdB"
                     /locus_tag="J1Y83_002047"
                     /gene_synonym="rodA"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004885876.1"
                     /note="rod shape-determining protein RodA; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase MrdB"
                     /protein_id="HBA7630194.1"
                     /translation="MTDNPNKKTFWDKVHLDPTMLLILLALLVYSALVIWSASGQDIG
                     MMERKIGQIAMGLVIMVVMAQIPPRVYEGWAPYLYIICIILLVAVDAFGAISKGAQRW
                     LDLGIVRFQPSEIAKIAVPLMVARFINRDVCPPSLKNTAIALVLIFMPTLLVAAQPDL
                     GTSILVALSGLFVLFLSGLSWRLIGVAVVLVAAFIPILWFFLMHDYQRQRVMMLLDPE
                     SDPLGAGYHIIQSKIAIGSGGLRGKGWLHGTQSQLEFLPERHTDFIFAVLAEELGLVG
                     ILILLALYILLIMRGLWIAARAQTTFGRVMAGGLMLILFVYVFVNIGMVSGILPVVGV
                     PLPLVSYGGSALIVLMAGFGIVMSIHTHRKMLSKSV"
     gene            24256..25344
                     /gene="rlpA"
                     /locus_tag="J1Y83_002048"
     CDS             24256..25344
                     /gene="rlpA"
                     /locus_tag="J1Y83_002048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic peptidoglycan transglycosylase RlpA"
                     /protein_id="HBA7630195.1"
                     /translation="MRKQWLGICIAAGMLAACTSDDGQQQTVSVPQPAVCNGPIVEIS
                     GADPRFEPLNATANQDYQRDGKSYKIVQDPSRFSQAGLAAIYDAEPGSNLTASGEAFD
                     PTQLTAAHPTLPIPSYARITNLANGRMIVVRINDRGPYGNDRVISLSRAAADRLNTSN
                     NTKVRIDPIIVAQDGSLSGPGMACTTVAKQTYALPAPPDLSGGAGTSSVPVPQGEILP
                     VSNSTLKSEDPTGAPVTSSGFLGAPTTLAPGVLEGSEPTPAPQPVVTAPSTTPATSPA
                     MVTPQAASQSASGNFMVQVGAVSDQARAQQYQQQLGQKFGVPGRVTQNGAVWRIQLGP
                     FASKAEASTLQQRLQTEAQLQSFITTAQ"
     gene            25484..26695
                     /gene="dacA"
                     /locus_tag="J1Y83_002049"
     CDS             25484..26695
                     /gene="dacA"
                     /locus_tag="J1Y83_002049"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase DacA"
                     /protein_id="HBA7630196.1"
                     /translation="MNTIFSARIMKRLALTTALCTAFISAAHADDLNIKTMIPGVPQI
                     DAESYILIDYNSGKVLAEQNADVRRDPASLTKMMTSYVIGQAMKAGKFKETDLVTIGN
                     DAWATGNPVFKGSSLMFLKPGMQVPVSQLIRGINLQSGNDACVAMADFAAGSQDAFVG
                     LMNSYVNALGLKNTHFQTVHGLDADGQYSSARDMALIGQALIRDVPNEYSIYKEKEFT
                     FNGIRQLNRNGLLWDNSLNVDGIKTGHTDKAGYNLVASATEGQMRLISAVMGGRTFKG
                     REAESKKLLTWGFRFFETVNPLKVGKEFASEPVWFGDSDRASLGVDKDVYLTIPRGRM
                     KDLKASYVLNSSELHAPLQKNQVVGTINFQLDGKTIEQRPLVVLQEIPEGNFFGKIID
                     YIKLMFHHWFG"
     gene            26805..27068
                     /locus_tag="J1Y83_002050"
     CDS             26805..27068
                     /locus_tag="J1Y83_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbeD family protein"
                     /protein_id="HBA7630197.1"
                     /translation="MKTKLNELLEFPTPFTYKVMGQALPELVDQVVEVVQRHAPGDYT
                     PTVKPSSKGNYHSVSITINATHIEQVETLYEELGKIDIVRMVL"
     gene            27169..27810
                     /gene="lipB"
                     /locus_tag="J1Y83_002051"
     CDS             27169..27810
                     /gene="lipB"
                     /locus_tag="J1Y83_002051"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415163.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HBA7630198.1"
                     /translation="MYQDKILVRQLGLQPYEPISQAMHEFTDTRDDSTLDEIWLVEHY
                     PVFTQGQAGKAEHILMPGDIPVIQSDRGGQVTYHGPGQQVMYVLLNLKRRKLGVRELV
                     TLLEQTVVNTLAELGIEAHPRADAPGVYVGEKKICSLGLRIRRGCSFHGLALNVNMDL
                     SPFLRINPCGYAGMEMAKISQWKPEATTNNIAPRLLENILALLNNPDFEYITA"
BASE COUNT         6835 a         7321 c         7204 g         6683 t
ORIGIN      
        1 tgtgtcgaat atgttcggca aattcaaaaa ccaatttgtt ggggtatcgc caagcggtaa
       61 ggcaccggat tctgattccg gcattccgag gttcgaatcc tcgtacccca gccacattaa
      121 aaaagctcgc ttcggcgagc tttttgcttt tctgcgtata ttcaatgccg aatgcgatgt
      181 tgactcgtct tatccttcaa agtcggatgc gacgctgccg cgtcttatcc gacctacggt
      241 tggcatgcat ccagcaatgt tgtgaggcta caaccctaac gcatatttca gcgcctgacg
      301 tttcaacacg ccagcacgct ccgccgccat caaaccaaga ttacgcacaa aacgcagcgg
      361 tggcagatta ttgctgaatc cggcataaaa cagatccata ccactttgca tcatgaagtt
      421 atccgccata cgccgcatct ggtaacgctt gagcacagga taactggccc acgcttcgcc
      481 gtagctgcgg gcgttcacca gaacgtcaat cagggcatcg acatcacgat aaccaagatt
      541 caccccctgc cccgccagcg gatggatggt atgcgcggca tcgcccacca gcgcaagccc
      601 cggctgcaca tactgcaacg catggcgacg tatcagcgga aacgcgccag cggcaagcgg
      661 tgttacatac ccaagacgcg acgggaaatg cttcacgatt tccgcctgta gctgcgccat
      721 attcatattc tgcaactggc gaatgcgcgc cggagagtca taccacacca gcgatgccca
      781 gttatcaaac aacggcagaa acgcacgcgg tccgtccggg gtaaattgct gccaggtgct
      841 gtcgccggga tcgttctcac actgtacgct aatcaacata cacgactgcg catactgcca
      901 ggcatgaacg ccaattcccg ccatctgtcg cacctgcgaa tttgcgccgt cggcaccaat
      961 caccagcttc gcgaggatcg tttcaccgcc cttcagttcc agctcctgaa gatcattatg
     1021 gcgatgcagt gcaatcagcg aggttggcac acgtaacgtt actttcggat gcgcttccag
     1081 cgcctgccac aacgcctgtt gcaggacagt gttttccacc atgtagccaa gcaacggtag
     1141 cttaagttca gcggcgtcaa acacgacatg cgccgtttcc cactcccacg tttccagtct
     1201 gcggtaagga tggcaacgca tagcctgtac tgcatcccag acccctaccc ctttaagcaa
     1261 tgataccgaa gccgcgctga tcgccgagat ccgcacgtcc ggctggctgt ccgcaacaaa
     1321 cggcgcaggc tgggcatgtt caataaccgt taccgtaaac ccgtgctgtg ccagccccag
     1381 cgccagtgcg ccgccgacca ttcctccgcc gacaatggca atttccgttg gttgatttgt
     1441 catggtcaat catcctgtta gcaatatgct ttaagtttac aggattttta cccccaaggg
     1501 ctgataacgc tcatactggt cacaacggca gcaaagcatt acactatgcg ccctgcattc
     1561 ctggctacta tttcgcaaga gcaagtcgat gaccaaaaaa ctccatatta aaacctgggg
     1621 ctgtcagatg aacgagtacg attcatcgaa gatggccgat ctgctggatg ccacccacgg
     1681 ctatcaactg accgacgtgg cggaagaagc ggatgtgctg ctgctgaaca cctgctcaat
     1741 ccgcgagaag gctcaggaaa aagtcttcca tcagttgggt cgctggaaac tgttaaaaga
     1801 gaagaatcca gacctgatta tcggcgtcgg cggctgcgtg gcatcgcaag aaggcgagca
     1861 cattcgccag cgcgcccact atgtcgatat tatttttggg ccgcaaacgc tgcatcgtct
     1921 accggagatg atcaactccg tgcgcggcga ccgcagcccg gttgtagata tcagcttccc
     1981 ggaaatcgag aagtttgacc gtctgccaga accgcgcgcc gaagggccga ccgcgtttgt
     2041 ctccatcatg gaaggctgca ataaatattg cacctactgc gtggtgcctt acacccgtgg
     2101 tgaagaggta agccgtccgt ccgacgatat tctgtttgag atcgcccagc ttgcggctca
     2161 gggcgtgcgt gaagtcaacc tgctcggtca gaatgtgaat gcctggcgtg gtgagaacta
     2221 cgacggcacc accggaacgt ttgccgatct gctgcgtctg gttgctgcga tcgacgggat
     2281 cgatcgtatt cgctttacca ccagccatcc gatcgaattc accgacgata tcatcgaagt
     2341 gtaccgcgac acgccggagc tggtgagctt cctgcatctg ccggtacaga gcggttccga
     2401 tcgcattctg aacctgatgg gccgtactca cacggcgctg gagtacaaag cgatcatccg
     2461 taaactgcgt gcggcgcgtc cggatattca gatcagttct gacttcatcg ttggcttccc
     2521 tggcgaaacc accgaagact tcgagaaaac gatgaagctg attgccgacg tcaatttcga
     2581 catgagctac agctttatct tctctgcacg tccgggtacg ccagccgccg atatggttga
     2641 tgatgttccg gaagaagaga agaagcagcg tctgtatatt ctgcaagaac gtattaatca
     2701 gcaagcgatg gcatggagcc gccggatgct cggcaccacc cagcgcattc tggtagaagg
     2761 gacttcacgt aagagcatca tggagctttc cggtcgcact gaaaataacc gcgtggttaa
     2821 cttcgagggc acgccggata tgatcggtaa attcgtcgat gtagaaatta ccgacgtcta
     2881 cccgaactct ctgcgcggta aagtggtgcg aactgaagat gaaatgggtc tgcgcgtggc
     2941 agaaacaccg gaatcggtga ttgcccgtac ccgcaaagaa aacgaccttg gcgtgggtta
     3001 ttatcagccg taattctcag gcccgccgtt ccggtgggcc ttgttttttc tctctttatc
     3061 cccatcttta tcgcaatgct tgcgcctttt tcctgtggcg tgaataattt ccttataggc
     3121 cggtgaatgt tcagcttccg gcggcataca gataagagga acggtttgaa catagacact
     3181 cgcgaaatca ccctggagcc agcagacaat gcgcgtctgt tgagcctgtg cggcccgttt
     3241 gatgacaaca tcaaacaact cgaacgccgt ctcggcatcg aaatcaatcg ccgcgataac
     3301 cactttaaac tgaccggccg tccgatttgc gtcaccgctg cggcagacat tctgcgtagc
     3361 ctgtatgtcg atactgcccc gatgcgcggt cagattcagg atatcgaacc ggaacagatc
     3421 caccttgcga ttaaagaagc gcgggtactg gagcaaagcg cggagagcgt gccagagtac
     3481 ggcaaagcgg tcaatatcaa aaccaaacgc ggcgtaatta agccgcgcac gccaaaccag
     3541 gcgcagtaca tcgccaatat tctcgaccat gacattacct tcggcgttgg cccggcgggt
     3601 acgggtaaaa cctacctggc agtggctgcg gcagttgatg ccctggagcg tcaggaaatt
     3661 cgccgtattc tgctgactcg tccggcagta gaagccggtg agaaactggg cttcctgcct
     3721 ggcgatttaa gccagaaagt agacccgtat ctgcgcccgc tgtacgacgc gctgtttgaa
     3781 atgctgggct ttgagaaagt cgagaaactg attgagcgca acgttattga agtcgcaccg
     3841 ctggcctata tgcgtggtcg tacgctgaac gacgcgttta tcattcttga tgagagccag
     3901 aacaccacca tcgaacagat gaagatgttc ctgacccgta tcggttttaa ctcaaaagcg
     3961 gttatcaccg gcgacgtcac acagatcgac ctgccgcgta ataccaagtc tggtttgcgt
     4021 catgccatcg aagtgctggc cgatgtcgaa gagatcagct ttaacttctt ccacagcgaa
     4081 gacgtggttc gtcacccggt ggtggcgcgt atcgttaacg cctatgaagc ctgggaagaa
     4141 gccgaacaaa aacgtaaagc ggcgctggca gcagaacgca agcgcgaaga acaggaacaa
     4201 aaatgagtca ggtgatcctc gatttacaac tggcatgtga agataattcc gggttaccgg
     4261 aagagagcca gtttcagaca tggctgaatg cggtgatccc gcagtttcag gaagaatcgg
     4321 aagtgactat tcgcgtggtc gataccgccg aaagccacag tctgaatctg acctatcgcg
     4381 gtaaggataa gccgaccaac gtgctctcct tcccgtttga agtaccgcct ggcatggaaa
     4441 tgtcgctact gggcgatctg gttatctgcc gtcaggtggt tgagaaggaa gctcaggagc
     4501 aaggcaaacc actggaggcg cactgggcgc atatggtggt gcacggcagt ctgcatttgt
     4561 taggttacga tcacatcgaa gatgacgaag cagaagaaat ggaagccctc gaaacagaga
     4621 ttatgcttgc tctgggctat gaggatccgt acattgccga gaaagaataa tttgccaggc
     4681 atttgactgg cagacgtccc cgccgttggt gattaacgac ggcgttttga ttaactaaca
     4741 tgagaaccca tacgacgcca tgagcgacga caattcacac agtagtgaca cgataagcaa
     4801 caagaaggga tttttctccc tgttactcag ccaacttttc cacggtgaac cgaaaaaccg
     4861 tgacgaactg ctggcgctga tccgtgattc cgggcagaac gaccttatcg acgaagatac
     4921 gcgcgatatg ctcgaagggg tgatggacat cgcagaccaa cgcgttcgcg acatcatgat
     4981 cccccgctcc cagatgatta ccctgaaacg caaccagacg ctggacgaat gccttgatgt
     5041 catcattgag tccgcccact cacgtttccc ggtgattagc gaagacaaag atcacattga
     5101 agggattctg atggcgaaag acttgctgcc gtttatgcgc agcgatgctg aagccttcag
     5161 catggacaaa gtgttacgtc aggcggttgt cgttcctgaa agtaagcgcg tagaccggat
     5221 gctgaaagag tttcgctctc agcgttacca catggcgatc gtcattgacg aattcggtgg
     5281 ggtttccggt ctggtgacca ttgaagacat cctggaactg attgttggtg agattgaaga
     5341 cgagtatgac gaagaagatg atatcgactt ccgtcagctg agtcgtcata cctggaccgt
     5401 gcgcgcactg gcttctattg aagacttcaa cgaagcgttc ggcacccact ttagcgatga
     5461 agaggttgac actatcggtg gtctggtgat gcaggcattt gggcatcttc cggcgcgtgg
     5521 cgaaaccatc gacatcgacg gttaccagtt caaagtggcg atggccgaca gtcggcgtat
     5581 tattcaggtt catgtcaaaa tcccggatga ctcaccccag ccgaagctgg atgaataaaa
     5641 ccgaaactgg atagataact acatggcttt tgcctcatta attgaacgcc agcgcattcg
     5701 cctgctgctg gcgttattat tcggtgcctg cggaacgctg gccttctctc cttacgacgt
     5761 ctggcctgcg gcgattattt cgctgatggg gcttcaggcg ttgaccttta accgccgtcc
     5821 actccagtct gccgctattg gcttttgctg gggatttggc ctctttggca gcggtattaa
     5881 ctgggtctat gtcagcatcg cgacctttgg cggaatgcct ggcccggtta acatcttcct
     5941 ggtggtactg ctggcggcgt atttgtcgct gtataccgga ctgtttgctg gcgtgctgtc
     6001 gcgtctgtgg ccgaaaacca cctggctgcg cgtggcgatt gccgcccctg ctctctggca
     6061 agtgaccgag tttctgcgcg gttgggtact aacaggcttc ccgtggttac agttcggcta
     6121 tagccagatt gatggcccgt taaaagggct ggcaccgata atgggcgtgg aagccattaa
     6181 cttcctgctg atgatggtta gtggcctgct ggcactggcg ttggtcaaac gcaactggcg
     6241 tccgctggtg gtggccgtcg tgctgtttgc ccttcccttc ccgctgcgtt acatccagtg
     6301 gtttaccccg caaccggaaa aaactattca ggtttcgatg gttcagggcg atattccgca
     6361 atcgctgaaa tgggacggag accagctact taatacgctg aagatttact acaacgcaac
     6421 ggcaccgctg atgggcaaat catcgttgat tatctggccg gagtcggcga taaccgatct
     6481 ggaaattaat cagcaaccgt tcctcaaagc tctggacggt gagttgcgcg ataaaggtag
     6541 ctcgctggtc accgggattg tcgacgcgcg tctcaataaa cagaaccgct acgataccta
     6601 caacaccatc atcacactgg gtaaaggcgc accgtacagc tatgaatcag ccgatcgcta
     6661 taacaaaaac catctggtgc cgtttggcga gtttgtcccg ctagagtcga ttctgcgtcc
     6721 gttagcgccg ttctttgatc tgccgatgtc ttcattcagc cgcggaccat atattcagcc
     6781 accgctgtcg ttgaatggca ttcaacttac tgcggccatt tgctacgaaa tcattctcgg
     6841 cgagcaagtg cgcgataact tccgcccgga taccgactat ctgctgacta tctccaacga
     6901 tgcgtggttt ggtaagtcta ttggtccatg gcagcacttc cagatggcgc gaatgcgtgc
     6961 gctggagctg gcgcgcccac tgttgcgcag caccaacaac ggcattacgg cggtgattgg
     7021 cccgcagggt gagattcagg cgatgatccc gcagttcacc cgcgaggtgt taaccactaa
     7081 cgtaacgccg accaccggac tcaccccgta cgcacgtacc ggcaactggc cactgtgggt
     7141 gctgacggca ctgtttggtt ttgcagctgt gctgatgagt ctgcgtcagc gacgtaaacg
     7201 ttgatgtgaa tgatgcgctg gcctgcaaaa ttgcatcaat tttgcaggcc agataaaccg
     7261 ttcacaccgc atccggtatc aacaaaacat actttctcat tgagttgtgg ggcatgatta
     7321 tcatgccctt ttttcttacg gttcagggat acaagccaca tcgccatatt ggggatgccg
     7381 aataattgcc tgcggatcaa gtgaactttc tctgtccaat tgtattagcc cgttcttcgc
     7441 cataacaaat atctttctac catccgctga aaaacgaaga ttattaacgt agctggcagg
     7501 ataaacgcca ataatctgcc cggcattaat attgagcagg atgacataac cttgcaggcc
     7561 atatgacacc aacgctaaat actgaccatc cgggctaata ccctcaacgg aataaacgct
     7621 cctgctacac cattgcgtca taatgctggc ttgttgatcc tcagctctta tacgtgaaat
     7681 cccctggcta tcggcaaaaa taatctcacc attttttggt cggaatccca tcgcagaaac
     7741 gacaccacgc gcatcacctg cgggaaagtc ttctttctga tgcgtgatgg tattgatccg
     7801 cacgatgtta cctctgcctg ctcccggaac cagtaatgtt ttttcatccg gggaaagtgc
     7861 cataacccag gcgcctggtt taccgctcgt ccccgtcccg tcgtatttct ccagtgggat
     7921 atccgccaca tgctgataag tcaccagatc aatcgctgac acactattac tggtgcgatt
     7981 catcacccag gcttttgtgc catcacgcga gaggataagg ctgtcaggat gggcgccggt
     8041 ggtaatcgac tgaataatct cccccgtcgc cacgttaatg accgatactg cattcgcttc
     8101 aaatgaagtc gcataaatca acttttcatc caggcttaat gccagcgatc tgacgccctg
     8161 atctttacag ccaccacaat gtttgactga cttttctttc gctatgggcg ctgtaaaggt
     8221 atgcaggatt ttcttttgtc cgaggtcgaa aacaacaatg ctgttcgtgt tttcaattgc
     8281 ggcatacaac gtattaccat cccgactgat cgccatatca tggatatcgg caatctcagc
     8341 gagtaaatca ccactggcct gctcacgact taccggagcg gataatgttt ccctgagggc
     8401 gttgcgctcc tccactaact gctgcttttt ttgtcgtcgc tcttgttcaa tctcctgcgg
     8461 ataaaacgct tcacgcaggc tttgcctgac ctgtaaaact gattcatcaa tctgctgctg
     8521 cacaaaccag atagtcccag tcagaagaag agtgaccgca acaataatcc cgccttccgt
     8581 aacgagccga ttttgcccaa ctaaacgcgc actcagccaa cccaacgcgc cccccgcaat
     8641 caggccgcca atgtggcaag cgttatctat tcctgtttgc cgggtaccat acaacagtgt
     8701 cagcgccacc atacccaaca gaggaaatac tcgccgctgg tttttatggt gtgtgcccgc
     8761 tattcccata attgccccgg atgccccaac gctgatgact atctgctcac tttcgcgtaa
     8821 cgcataacag gcgctgatca gcccaccaaa gatgccggag aataaccaga taatcaggag
     8881 tcgccatttc cccagtatcc gttccgctac tgtccccacc gccaggagtg caaacatatt
     8941 catcagcaaa tgggaaaaac tactgtgcag aaaaacactg ctcaataagc gccaggattc
     9001 atccgacaac gtgagagaag caacgttagc gccgagatgg atgagtgcgg attgatcgac
     9061 ttcttcgtaa ctcatccatt gtgcgaggta acaaaatatc ccaacacaga gcgcaaaaag
     9121 gatcagggtt attgcgggta attgaacatt taacggctta accgaagatg cagacataag
     9181 gtgattcttc cttgaaaaga ctagtaaaaa gcattttccc gtgtgtagtt atcatcccgg
     9241 caaacgtccc acaccgaaaa gttaacactg tccgaaatat gactttttca aactgaaatt
     9301 acaatcgtaa ttaatgcacc cctaagtatt ttttggcatg taagttgctt tatttactgt
     9361 gtaagcaatg aaatcagccg caacgcaacc gggttgcacc accacaaatc agcggtagca
     9421 ccaaaaaggt gcggtttaag aattttgctt cggaatggtg cggcgcacct cgcaaaaata
     9481 aacaataaaa cagcaatatc tttacattta tccaaactaa atgttaccta acaaacacaa
     9541 cactgcacaa taaagtcgca gatgataaca acacaaacac tcacaacggg tatccatgcg
     9601 tttttaacgc agaagataaa ggagttggat atgcaattac gtaaacctgc cacagcaatc
     9661 ctggccctgg cgctttccgc aggactggca caggcagatg acgccgcccc ggcagcaggc
     9721 agcacgctgg acaaaatcgc caaaaacggc gtgattgtcg tcggtcaccg tgaatcttca
     9781 gtacctttct cttattacga caatcagcaa aaagtggtgg gctactcgca ggattactcc
     9841 aacgccattg ttgaagcagt gaaaaagaaa ctcaacaaac cagacttaca ggtgaaattg
     9901 attccgatca cctcacaaaa ccgtattcca ctgctgcaaa acggcacttt cgattttgaa
     9961 tgcggttcta ccaccaacaa tgccgaacgc caaaaacagg cggctttctc tgacactatt
    10021 ttcgtggtcg gtacgcgcct gctgaccaaa aagggcggcg atatcaaaga ttttgccgac
    10081 ctgaaaggca aagccgtagt cgtcacttcc ggcaccacct ctgaagtttt gctcaacaaa
    10141 ctgaatgaag agcaaaaaat gaatatgcgc atcatcagcg ccaaagatca cggtgactct
    10201 ttccgtaccc tggaaagcgg tcgtgccgtt gcctttatga tggatgatgc tctgctggcc
    10261 ggtgaacgtg cgaaagcgaa gaaaccagac aactgggaaa tcgtcggcaa gccgcagtct
    10321 caggaagcct atggttgcat gttgcgtaaa gacgatccgc agttcaaaaa gctgatggat
    10381 gataccatcg ctcaggtgca gacctccggt gaagcggaaa aatggtttga taagtggttc
    10441 aaaaatccaa ttccgccgaa aaacctgaac atgaatttcg aactgtcaga cgaaatgaaa
    10501 gcactgttca aagaaccgaa tgacaaggca ctgaactaat tacaagaacc aggggcggaa
    10561 attccagccc tctcgattgt tacgtagcac ggacagacta tacgcctggt ggtcgttccc
    10621 catcgggcct gaaaaccgca atacgctggg taacaatctt cgagggtagc agttaacgct
    10681 gctaccctct tttttctgga gtagatttat gtctatagac tggaactggg gtattttttt
    10741 acaacaagcc ccgttcggca acaccaccta tcttggttgg atctggagtg gctttcaggt
    10801 gacgatcgct ttgtcgatct gcgcctggat tatcgctttc ctcgtcggtt catttttcgg
    10861 cattttacgt accgttccaa accgttttct ctccggtctg ggtacgttgt atgtcgaact
    10921 gttccgcaac gtgccgttga ttgtgcaatt ctttacctgg tacctggtga tcccggagct
    10981 gctgccagag aaaatcggca tgtggtttaa ggcagagctg gatcccaata ttcagttttt
    11041 cctttcatcc atgctctgcc tggggctgtt taccgccgcc cgtgtttgcg aacaggtacg
    11101 cgccgctatt cagtcgctgc cgcgtgggca aaaaaatgcc gcgctggcga tgggtctgac
    11161 gctaccgcag gcttaccgct acgtactgct gcctaatgct tatcgcgtta tcgtcccgcc
    11221 gatgacctca gagatgatga acctggtgaa aaactccgcc atcgcctcga ctatcggtct
    11281 ggtggatatg gcggcgcagg cgggtaaatt gctcgattac tcggcccacg cctgggagtc
    11341 gtttaccgcc atcacgctgg cgtatgtatt gattaacgct tttatcatgc tggtgatgac
    11401 gctggttgaa cgtaaagttc gcctgcccag caacatgggg ggcaaataat gtacgagttt
    11461 gactggagtt ccattgttcc ttccctgcca tatctgctcg acgggctggt gatcacgctg
    11521 aaaatcaccg tcacggcagt ggtgattggt attttgtggg gcaccatgct ggcggtgatg
    11581 cgcttgtcca gctttgcgcc cgtagcgtgg tttgccaaag cctacgttaa cgtattccgc
    11641 tcgattcctt tagttatggt tttgctgtgg ttttacctga tcgtaccggg ttttctgcaa
    11701 aacgtgctgg gattatcacc caaaaatgat atccgtttaa tttcggcgat ggtggcgttt
    11761 tcgatgtttg aagcggccta ctattcagag attatccgtg ctggtattca aagtatttct
    11821 cgtggtcagt cgagcgccgc gctggcgttg ggaatgactc actggcagtc gatgaaactg
    11881 attattctgc cgcaggcgtt ccgcgcgatg gtgccgctgt tgctcactca gggcatcgta
    11941 ctgtttcagg atacctcact ggtgtatgtg ttatctctgg ccgatttctt ccgtaccgcc
    12001 tcaaccattg gtgagcgtga tggtacgcag gttgagatga tcctgtttgc cggatttgtt
    12061 tatttcgtta ttagccttag cgcatcgttg ttggtcagct acttgaaaag aaggacagca
    12121 taatgattac cctgaaaaat gtttcaaaat ggtatggtca ctttcaggtg ctgaccgact
    12181 gctcaaccga agtgaaaaaa ggcgaagttg tggtggtttg cggcccgtct ggttcaggca
    12241 aatcaacgct gattaaaacc gtcaacggcc tcgaaccggt gcagcaaggt gaaatcaccg
    12301 tcgatggcat cgtggttaac gacaagaaaa ccgatctggc gaaactgcgt tcccgcgtcg
    12361 ggatggtgtt tcagcatttc gagctgttcc cccatctgtc gattatcgaa aacctgaccc
    12421 tggcgcaggt gaaagtgctt aaacgcaata aagcgccggc tcgtgaaaaa gcattgaaat
    12481 tgctggagcg cgtcgggctt tccgcacatg ccaataagtt tccggcgcag ctttccggcg
    12541 gccagcagca gcgtgtggcg atcgctcgcg cattgtgcat ggatcctatt gccatgctgt
    12601 tcgacgaacc aacgtcggca ctggatccgg agatgatcaa cgaagtgctg gacgtgatgg
    12661 tcgaactggc gaacgaagga atgacgatga tggtggtgac ccacgaaatg ggctttgccc
    12721 gtaaagtggc gaatcgggtg atctttatgg acgagggtaa aattgtcgaa gactcgccga
    12781 aagacgcttt cttcgatgat ccgaaatcgg accgcgcaaa agacttcctc gcaaaaatcc
    12841 tgcattaatc actctggcgc gcatactcaa ttgcgcgcca tctcacgctt tctcgtttcg
    12901 tccctcgtat ctgttacatc ccggcgttac ccttgtcgca aagaaacaca caacaaggag
    12961 caacaatggc actgccaatt ctgttagatt gcgacccagg tcatgacgac gctatcgcaa
    13021 tcgttctcgc cctcgcctca ccagagcttg atgtcaaagc aattacgtct tccgccggaa
    13081 accagacacc agaaaaaacc ttacgcaatg ttctgcgtat gctgaccttg cttaatcgca
    13141 ccgatattcc ggtagcaagc ggtgcggtaa aaccgttaat gcgtaatttg attattgcgg
    13201 acaacgtgca cggcgaaagc ggtctcgacg gcccggcatt accggaaccg acgttcgcac
    13261 cgcaaaactg tacggcggtg gagctgatgg cgaaaacgct gtgtgaaagt gaggaacctg
    13321 tcaccattgt ttctaccgga ccgcaaacta acgttgcctt gctgctcaat agccacccgg
    13381 aactgcatag caaaattgcc cgtatcgtga ttatgggcgg cgcaatgggg cttggtaact
    13441 ggacgcctgc ggctgaattt aatatttacg ttgacccgga agcggcagaa attgtcttcc
    13501 agtcaggaat tccggtggtg atggccggtc tggatgttac tcacaaagca caaatccacg
    13561 ttgaagacac tgagcgtttc cgcgcgattg gcaaccctgt ttcaaccatt gttgccgaac
    13621 tgctggattt cttcctcgaa tatcataaag acgaaaaatg gggctttgtc ggcgcaccat
    13681 tgcatgaccc atgcaccatc gcctggctgt tgaaaccgga gttgtttacc actgttgagc
    13741 gctgggttgg cgtggaaaca caagggaaat atacccaggg tatgacggtt gttgattatt
    13801 attatctgac tggcaataaa ccgaatgcca ccgtaatggt cgatgttgat cgtcagggct
    13861 ttgttgattt actggctgat cgtctgaaat tttacgctta atcactcgcc ggatgacatc
    13921 acgccatccg gcaccactca tttcattacg gctcgaacgg acgtctctgg aactgatcat
    13981 gaccacattt cgggcatagc gtcagcactt ccggtgtata gatcgggaga tggaagtgac
    14041 atttctcgca gaccagattt cccagcccta ccacttctcc gctgtgataa accccatgat
    14101 gattgaggtc ctggaaaact tcgcgccatt caagctgcgt tttatcggtg atgtctgcca
    14161 gctcctgcca caagctttct ttaatcaccc gcataaagac gctgtcagac tcttctttca
    14221 ggctctcttc atagctcatg gcgaactctt ccaggtcacg tctgacagct cgcgtcagct
    14281 catcgacctc ggttcgcgtt aactcccctg tttttattac gcgctcgcgc gcctgttcca
    14341 ccagtgcgtc gatatcacgt tcgccattgc gcaggcgttc gctcagtgac gcgaccagtt
    14401 cacggtaata ttgagcaacc ttgttcatcg tttcatctcc cgggtcgtta actgactata
    14461 ataatagacg ttctttgttc cttgctttgc taatacgccc acaaactctg taaattaatg
    14521 tatgaataat ccgggcatca tttcaacgtc gagtgcgcga aaggctgttt tgacgcgggc
    14581 gtttgggcta tgctatgcgg atctgaaaaa ccacatcaac gctacatttg tagccgtatt
    14641 gaaaacagga ccactggctg ccatgcaaga gcaataccgc ccggaagaga tagaatccaa
    14701 agtacagctt cactgggatg agaagcgcac atttgaagta accgaagacg agagcaaaga
    14761 gaagtattac tgcctgtcta tgcttcccta tccttctggt cgactacaca tgggccacgt
    14821 tcgcaactac accatcggtg acgtgatcgc ccgctaccag cgcatgctgg gcaaaaacgt
    14881 tcttcagcca atcggctggg acgcatttgg tctgcctgcg gaaggcgcgg cggtgaaaaa
    14941 caacaccgcg ccagcaccgt ggacgtacga caacatcgcg tatatgaaaa accagctcaa
    15001 aatgctgggc tttggttatg actggagccg cgagctggcg acctgtacgc cggaatacta
    15061 ccgttgggaa cagaaattct tcaccgaact gtataaaaaa ggcctggtat ataagaagac
    15121 ttctgcggtc aactggtgcc cgaacgacca gaccgtactg gcgaacgaac aagttatcga
    15181 cggctgctgc tggcgctgcg ataccaaagt tgagcgtaaa gagatcccgc agtggtttat
    15241 caaaatcact gcttacgctg acgagctgct caacgatctg gataaactgg atcactggcc
    15301 agacaccgtt aaaacaatgc agcgtaactg gatcggtcgt tccgaaggcg tggaaatcac
    15361 cttcaacgtt aacgactatg acaacacgct gaccgtttac actacccgcc cggacacctt
    15421 tatgggttgt acctacctgg cggtggctgc gggtcatccg ctggcacaga aagcggcgga
    15481 aaataatcct gaactggccg cctttattga cgaatgccgt aacaccaaag ttgccgaagc
    15541 tgaaatggcg acgatggaga aaaaaggcgt cgatactggc tttaaagcgg ttcacccatt
    15601 aacgggcgaa gaaattcccg tttgggcagc aaacttcgta ttgatggaat acggcacggg
    15661 cgcagttatg gcagttccgg gtcacgacca gcgcgactac gagtttgcct ctaaatacgg
    15721 cctgaacatc aaaccggtta tcctggcagc tgacggctct gagccagatc tttctcagca
    15781 agccctgact gaaaaaggcg tgctgttcaa ctctggcgag tttaatggtc ttgaccatga
    15841 agcggccttc aacgccatcg ccgataaact gactgcgatg ggcgttggcg agcgtaaagt
    15901 gaactaccgc ctgcgcgact ggggtgtttc tcgtcagcgt tactggggcg cgccgattcc
    15961 gatggtgacg ctggaagacg gtaccgtaat gccgaccccg gacgaccagc tgccggtgat
    16021 cctgccggaa gatgtggtga tggacggcat taccagcccg attaaagcag atccggagtg
    16081 ggcaaaaact accgttaacg gtatgccagc actgcgtgaa accgacactt tcgacacctt
    16141 tatggagtcc tcctggtact atgcgcgcta cacttgcccg gagtacaaag aaggtatgct
    16201 ggattccaaa gcggctaact attggctgcc ggtggatatt tacattggtg gtatcgaaca
    16261 cgccattatg cacctgctct acttccgctt cttccacaaa ctgatgcgcg atgcaggcat
    16321 ggtgaactct gacgaaccag cgaaacagtt gctgtgtcag ggtatggtgc tggcagatgc
    16381 cttctactat gttggtgaaa acggcgaacg taactgggtt tccccggttg atgctatcgt
    16441 tgaacgtgac gagaaaggcc gtatcgtgaa agcgaaagat gcggcaggcc atgaactggt
    16501 ttataccggc atgagcaaaa tgtccaagtc gaagaacaac ggtatcgacc cgcaggtgat
    16561 ggttgaacgt tacggcgcgg acaccgttcg tctgtttatg atgtttgctt ctccggctga
    16621 tatgactctc gaatggcagg aatccggcgt ggaaggggct aaccgcttcc tgaaacgtgt
    16681 ctggaaactg gtgtacgagc acacagcaaa aggtgatgtt gcggcactga acgtcgatgc
    16741 gctgactgaa gatcagaaag cgctgcgtcg cgatgtgcat aaaactatcg ctaaagtaac
    16801 cgatgatatc ggccgtcgtc agaccttcaa caccgcaatt gcggcgatta tggagctgat
    16861 gaacaaactg gcgaaagcac cgaccgatgg cgagcaggat cgcgccctga tgcaggaagc
    16921 gctgctggcg gtagtccgta tgcttaaccc gttcaccccg cacatctgct tcacgctgtg
    16981 gcaggaactg aaaggcgaag gcgatatcga caacgcgccg tggccggttg ctgacgaaaa
    17041 agcgatggtg gaagactcca cgctggtcgt ggtgcaggtt aacggtaaag tccgtgcaaa
    17101 aatcaccgtt ccggtggacg caacggaaga acaggttcgc gaacgtgctg gccaggaaca
    17161 tctggtagca aaatatcttg atggcgttac tgtacgtaaa gtgatttacg tacccggtaa
    17221 actcctcaat ctggtcgttg gctaagcgcg ggaggaagcg tgcgatatct ggcaacattg
    17281 ttgttatctc tggcggtgtt aatcaccgcc gggtgtggct ggcatctgcg tgacaccacg
    17341 caggttcctt ccactatgaa ggtcatgatc ctggactcag gcgatccgaa cgggccatta
    17401 agccgtgcgg tgcgtaatca gttacgtctg aatggtgtcg agttgcttga taaagaaacc
    17461 acgcgtaagg acgttccatc cttgcgtttg ggtaaagtga gcatcgcgaa agataccgca
    17521 tcggtattcc gtaacggtca aacagcagag tatcagatga tcatgacggt gaatgcgacc
    17581 gtgttgatcc ccggccgtga tatctacccg attagcgcca aagtcttccg ttcgttcttc
    17641 gataacccgc aaatggcgtt agcgaaagat aacgaacaag acatgatcgt aaaagagatg
    17701 tacgaccgtg ctgccgaaca gctgattcgt aagctgccaa gcatccgtgc tgcggatatt
    17761 cgttccgacg aagaacagac gtcgaccaca acggatactc cggcaacgcc tgcacgcgtc
    17821 tccaccacgc tgggtaactg atgattcggt tgtacccgga acaactccgc gcgcagctca
    17881 atgaagggct gcgcgcggcg tatcttttac ttggtaacga tcctctgtta ttgcaggaaa
    17941 gccaggacgc tgttcgtcag gtcgctgcgg cgcaagggtt cgaagaacac cacacttttt
    18001 ccattgatcc caacaccgac tggaatgcga ttttttcgtt atgccaggcc atgagcctgt
    18061 ttgccagtcg acaaacgcta ttgctgttgt tacccgaaaa cggaccgaat gcggcgatca
    18121 atgaacaact tctcacactc accggacttc tgcatgacga tctactgttg atcgtccgcg
    18181 gtaataaatt aagcaaagcg caagaaaatg ccgcctggtt tactgctctt gcgaatcgca
    18241 gcgtgcaggt gacctgtcag acaccggagc aggctcagct gccccgctgg gttgctgcgc
    18301 gcgcaaaaca gctcaactta gaactggatg acgctgcaaa tcaggtgctc tgctactgtt
    18361 atgaaggtaa cctgctggcg ctggcccagg cactggagcg tttatcgcta ctctggccgg
    18421 acggcaaact gacattaccg cgcgttgaac aggcggtgaa tgatgccgcg catttcaccc
    18481 cttttcattg ggttgatgct ttgttgatgg gaaaaagtaa gcgcgcgttg catattcttc
    18541 agcaactgcg tctggaaggc agcgaaccgg ttattttgtt gcgcacatta caacgtgaac
    18601 tgttgttact ggttaacctg aaacgccagt ctgcccatac gccactgcgt gcgttgtttg
    18661 ataagcatcg ggtatggcag aaccgccggg gcatgatgag cgaggcgtta aatcgcttaa
    18721 gccagccgca gttacgtcag gccgtgcaac tcctgacacg aacggaactc accctcaaac
    18781 aagattacgg tcagtcagtg tgggcagagc tggaagggtt atctcttctg ttgtgccata
    18841 aacccctggc ggacgtattt atcgacggtt gatatgaaat ctttacaggc tctgtttggc
    18901 ggcacctttg atccggtgca ctatggtcat cttaaacccg tggaaacgct ggcgaatttg
    18961 attggtctga cgcgggtcac aatcatccct aataatgttc ctccgcatcg tccccagccg
    19021 gaagcgaaca gcgtgcagcg taaacacatg cttgaactgg cgattgccga caagccatta
    19081 tttactcttg atgaacgcga gctaaagcgc aatgccccct cttacactgc gcaaacactg
    19141 aaagagtggc ggcaggaaca agggccagac gtgccgctgg cgtttattat tggtcaggat
    19201 tcattgctga cctttccgac ctggtacgaa tacgaaacga tactcgacaa tgcacatttg
    19261 atcgtctgtc ggcgtccagg ttacccactt gaaatggcac aaccgcaata ccagcaatgg
    19321 ctggaagatc atttgacaca taacccggaa gatcttcacc ttcagcctgc cggtaaaatt
    19381 tatctggctg aaacgccgtg gtttaacatc tcggcgacca tcatccgcga acgtttgcaa
    19441 aacggtgaat cgtgtgagga tttattaccg gaaccggtac tgacttacat taaccaacaa
    19501 ggcttgtatc gctgatacct gtcgttacgg agggcatgat gcgactgtgg ttaattcgtc
    19561 atggtgaaac gcaagcgaat gtcgatggtc tgtacagcgg tcatgcgccc acacccctga
    19621 ccgcgcgcgg tattgagcaa gcgcaaaatc tgcatacgct gctacatgat gtttcctttg
    19681 acctggtttt atgcagtgaa ctggaacggg cacagcatac cgcgcgactg gttcttagtg
    19741 accgccagca ccccgttcat atcatccctg aactcaatga aatgtttttt ggcgactggg
    19801 agatgcgaca tcatcgtgac ctcatgcaag aagatgccga aaactatagc gcgtggtgca
    19861 atgactggca gcatgctatc cccacgaacg gtgaaggatt tcaggcattt tcgcaacgtg
    19921 tggaacgatt tatcgcaagg cttagtgaat atcagcacta tcagaatatt ttagtcgtca
    19981 gccatcaggg cgtactgagt ctgttaattg ctcgtttaat tggtatgcct gccgagtcta
    20041 tgtggcattt ccgcgttgac caggggtgtt ggagcgccat tgatatcaac caaaaattcg
    20101 cgacgctacg cgtcctcaat agccgtgcca tcggggtcga gaatgcatga cttttctgtt
    20161 tttttacggg taagccgcaa cgaccattga cagccccggg caggctgata ttctccgcag
    20221 ccagactttt tccgccagac acgactttgt agaaattgtt ttacaaaaat ggcgatgcaa
    20281 tctgcggcgc ggggtgggat gatagcccac tttcgaaagc cgattcggcg acaattgtcc
    20341 cgaaatcgcc tctggttcag gtatactgac agaccatttt tatctatttg attcacccag
    20401 ggggaaaact tgcagggtaa agcactccag gattttgtta tcgacaaaat tgatgacctc
    20461 aaaggtcagg acatcatcgc cttagacgtt cagggcaaat ccagcatcac cgactgcatg
    20521 atcatctgta cgggtacgtc cagccgccat gttatgtcca ttgctgatca cgttgtgcag
    20581 gagtctcgcg cagcgggcct attaccgctc ggcgtagaag gtgaaaacag cgccgactgg
    20641 attgtcgtgg atttgggcga tgtgattgtc catgtcatgc aggaagagag ccgtcgcctg
    20701 tatgaactgg aaaaactctg gagttaatgc gtgaagctgc aacttgtcgc cgtgggaacg
    20761 aaaatgccgg actgggtaca aaccggtttt accgagtacc tgcgtcgttt tccgaaagat
    20821 atgcccttcg agctgattga aattccggcc ggaaaacgcg gcaagaatgc ggacatcaag
    20881 cgcatactcg acaaagaggg tgagcagatg ttggcggccg caggcaaaaa ccgcattgtc
    20941 accctcgata ttccaggcaa gccctgggat acgccgcagt tagccgctga gctggaacgc
    21001 tggaagctgg atggtcgcga cgtcagtcta ctgattggcg ggcctgaagg gttgtcgcct
    21061 gcctgtaaag cggcggcgga gcagagctgg tcgctgtcgg cacttaccct tccccatccg
    21121 ctggttcgcg tgctggtcgc agagagtctg taccgggcgt ggagcatcac caccaaccat
    21181 ccttatcacc gtgagtgata agtgagcttt gagtagaaaa cgcagcggat gaaactacag
    21241 aactcttttc gcgactatac ggcagagtcc gcgctgtttg tgcgccgggc gctagtcgcc
    21301 tttttgggga ttttgctgct gaccggcgtg cttatcgcca acctgtataa tctgcaaatt
    21361 gttcgcttta ccgactacca gacccgctct aatgaaaacc gcattaagct ggtgcctatc
    21421 gcacccagcc gcggcattat ctacgaccgt aacggtatcc ctctggctct caaccgcact
    21481 atctaccaga tagaaatgat gccggagaaa gtcgataacg tgcagcaaac gctggacgct
    21541 ttgcgcagcg tggttgatct gaccgatgat gatattgctg cattccgaaa agagcgcgca
    21601 cgttcacacc gtttcacctc tattccggtg aaaactaacc tgaccgaagt acaagtagct
    21661 cgctttgccg tcaaccagta ccgttttcct ggcgtcgaag ttaaaggcta taaacgtcgt
    21721 tactatcctt acggttcagc gttgacccac gtcatcggct atgtgtcgaa aatcaacgat
    21781 aaagacgtcg aacgcctgaa taatgacggc aaactggcca actatgcggc aacgcatgat
    21841 atcggtaagc tgggcattga gcgctactat gaagacgtgc tgcacggtca gaccggttat
    21901 gaagaggttg aagttaacaa ccgtgggcgt gttatccgcc agttaaaaga agtaccaccg
    21961 caagccggac acgatattta cctgacgctg gatctcaaac tccagcaata tattgaaacg
    22021 ctgctggcgg gtagccgcgc agctgtggta gtcaccgatc cgcgtacagg tggggtgttg
    22081 gcgctggttt ccacgcctag ttatgaccca aacctgtttg ttgacggtat ctccagcaaa
    22141 gattattccg ccttgctgaa cgacccgaat acaccgctgg tgaaccgcgc cacacagggg
    22201 gtttatcctc ccgcgtctac agttaaaccc tatgttgcgg tttcggcatt gagtgccggg
    22261 gtgatcacgc gcaatacgac gctgtttgac cccggctggt ggcaactgcc agggtcggaa
    22321 aaacgttatc gtgactggaa aaaatggggc cacgggcgtc tgaatgtcac aagatcgctg
    22381 gaagaatctg cggatacctt cttctatcag gtggcctacg atatggggat cgatcgcctc
    22441 tccgaatgga tggggaaatt cggttatggt cattacaccg gtatcgacct ggcggaagaa
    22501 cgttccggca acatgcctac ccgcgaatgg aaacagaaac gctttaaaaa accgtggtat
    22561 cagggtgaca ccattccggt tggtatcggt cagggttact ggacagcgac cccaatccag
    22621 atgagtaagg cactgatgat cctgattaat gacggtatcg tgaaggttcc gcatttgctg
    22681 atgagcaccg ccgaagacgg caaacaggtg ccatgggtac aaccgcatga accgcccgtc
    22741 ggcgatattc attccggtta ctgggagctg gcgaaagacg gtatgtacgg tgttgctaac
    22801 cgccctaacg gtacggcgca taaatatttt gccagcgcac cgtacaaaat tgcggcgaaa
    22861 tccggtaccg ctcaggtctt cggtctgaaa gcgaacgaaa cctataatgc gcacaaaatt
    22921 gccgagcgtt tacgtgacca caaactgatg accgcctttg cgccatacaa caatccgcaa
    22981 gtggctgtcg ccatgattct ggagaacggt ggcgcgggtc cggcggttgg tacgctgatg
    23041 cgccagatcc tcgaccacat tatgctgggt gataacaaca ccgatctgcc tgcggaaaat
    23101 ccagcggttg ccgcagcgga ggaccattaa tcatgacgga taatccgaat aaaaaaacat
    23161 tctgggataa agtccatctc gatcccacaa tgctgctgat cttactggca ttgctggttt
    23221 acagcgccct ggttatctgg agcgccagcg gtcaggatat tggcatgatg gagcgtaaaa
    23281 tcggccagat tgcgatgggt ctggtcatca tggtggtgat ggcgcaaatt cctccacgcg
    23341 tttatgaagg ctgggccccc tatctctata tcatctgtat tattttgctg gtggcggtag
    23401 atgctttcgg tgccatctct aaaggcgctc aacgctggct ggacctcggt attgttcgtt
    23461 ttcaaccgtc ggaaattgcc aagatagccg taccgttgat ggttgcacgc tttatcaacc
    23521 gcgacgtttg cccaccttca ttgaagaata cagctatcgc gctggtactg atatttatgc
    23581 ccacgctgct ggtggctgca cagcctgacc tgggaacctc aatcctcgtt gcgctttccg
    23641 gcctgtttgt actgttcctc tctggcctta gctggcgtct gattggcgtc gcagtagtgc
    23701 tggtagcggc gttcattccg attctgtggt tcttcctgat gcatgattac cagcgccagc
    23761 gcgtaatgat gctcctggac ccggaatcag acccactcgg cgcgggctat cacattattc
    23821 agtctaaaat tgctattggc tccggcggat tacgcggcaa aggctggctg cacggcactc
    23881 agtcacagct tgaatttctc cccgaacgcc ataccgactt tatcttcgcg gtactggcgg
    23941 aagagctggg attagtgggc attttgattc tgctcgctct ttacattctg ctgatcatgc
    24001 gcgggctgtg gatagccgcc agagcgcaaa ccacctttgg tcgcgtcatg gctggcggct
    24061 taatgctgat attattcgtt tatgtcttcg taaatattgg tatggtaagc ggtattctgc
    24121 cggttgtagg ggttccgctc ccactggtca gttatggagg atcggcgcta attgtgctga
    24181 tggctgggtt cgggattgta atgtcaatcc acacccacag gaaaatgttg tcgaaaagcg
    24241 tgtaagaggt gcgcaatgcg taagcagtgg ctcgggatct gcatcgcggc aggaatgctc
    24301 gcggcatgta caagcgatga tggtcagcaa cagacggtaa gtgtaccgca gcctgcggta
    24361 tgtaacggcc ccatagttga aattagcggg gcagacccgc gtttcgaacc actgaacgcg
    24421 acggcaaatc aggattacca gcgcgacggt aaaagctaca aaatcgtgca ggatccgtct
    24481 cgatttagcc aggcgggact ggcggcaatc tatgatgccg aacccggcag taacctgacg
    24541 gcctctggcg aagctttcga tccgacacag ttgacggcgg cccatccaac gcttccaatc
    24601 cccagctacg ccagaatcac taacctggct aacgggcgaa tgatcgtggt gcgcattaat
    24661 gatcgcggcc cttatggcaa cgaccgcgtg atttcgcttt ctcgcgcggc agctgaccgt
    24721 cttaacacgt caaacaacac caaagttcgt atcgatccga ttattgtcgc ccaggatggt
    24781 tcgctttctg gtcctggtat ggcttgtacc acagtcgcca aacagactta cgccctgcca
    24841 gcacctcccg atttaagcgg tggcgcggga acaagttcag tgcctgtccc gcagggtgaa
    24901 attcttccgg tcagtaattc gacgctaaaa agcgaagatc cgaccggcgc gccggtaacc
    24961 agcagcggtt tcctcggcgc accaacgacc ttagcgcctg gtgtactgga aggcagcgaa
    25021 ccgacgcctg cgccacagcc cgttgttaca gctccgtcga caacgcctgc aacctcacct
    25081 gcaatggtga caccgcaagc cgcctcgcaa agcgccagcg gcaactttat ggtgcaagtc
    25141 ggggccgtta gcgatcaggc tcgtgcgcaa cagtaccaac agcaactggg acagaagttc
    25201 ggcgtccccg gtcgcgtaac tcaaaatggc gcggtctggc ggatccagct tggcccattc
    25261 gccagcaaag ccgaagccag taccttgcag caacgtttgc aaaccgaagc ccaattacag
    25321 tcatttatta ccaccgcgca gtagcgtaaa gcaggcatct gatgtgtcaa tttctgtaaa
    25381 tgacgttaac aaagtcacgc ggaaagtcag atgcctgccg gtagtgcatt tgctatagta
    25441 gggcactttt tttaattcca tcacggatgt cgtagttcag accatgaata ccattttttc
    25501 cgctcgtatc atgaagcgcc tggcgctcac cacggctctt tgcacagcct ttatctctgc
    25561 tgcacatgcc gatgacctga atatcaaaac catgatcccg ggtgtaccgc agatcgacgc
    25621 ggagtcctac atcctgattg actacaactc cggcaaagta ctcgccgaac agaacgctga
    25681 tgtccgccgc gatcctgcca gcctgaccaa aatgatgacc agttacgtta tcggccaggc
    25741 aatgaaagcc ggtaaattta aagaaactga tttagtcact atcggcaacg acgcatgggc
    25801 caccggtaac ccggtgttta aaggttcttc gctgatgttc ctcaaaccgg gcatgcaggt
    25861 tccggtttct cagctgatcc gcggtattaa cctgcaatcg ggtaacgatg cttgtgtcgc
    25921 gatggccgat tttgccgctg gtagccagga cgcttttgtt ggcttgatga acagctacgt
    25981 taacgccctg ggcctgaaaa atacccactt ccagacggta cacggtctgg atgctgatgg
    26041 tcagtacagc tccgcgcgag atatggcgct gatcggccag gcgttgatcc gtgacgtacc
    26101 gaatgaatac tcgatctata aagaaaaaga atttacgttt aacggtattc gccagctgaa
    26161 ccgtaacggc ctgttatggg ataacagcct gaatgtcgac ggcatcaaaa ccggacacac
    26221 tgacaaagca ggttacaacc ttgttgcttc tgcgactgaa ggccagatgc gcttgatctc
    26281 tgcggtgatg ggcggacgta cttttaaagg ccgtgaagcc gaaagtaaaa aattgctaac
    26341 ctggggcttc cgtttcttcg aaaccgttaa tcctctgaaa gtaggtaaag agttcgcctc
    26401 tgaaccggtt tggtttggtg attctgatcg cgcttcgtta ggggttgata aagacgtgta
    26461 cctgaccatt ccgcgtggtc gcatgaaaga tctgaaagcc agctatgtgc tgaacagcag
    26521 tgaattgcat gcgccgctgc aaaagaatca ggtcgtcgga actatcaact tccagcttga
    26581 tggcaaaacg atcgaacaac gcccgctggt tgtgctgcaa gaaatcccgg aaggtaactt
    26641 cttcggcaaa atcattgatt acattaagtt aatgttccat cactggtttg gttaaaaatt
    26701 gaacacttga aagtgtaatt tccgtcccca tatactaagc atcagtaaaa aactcccgcc
    26761 ttctggcggg agttgctatt taattacgtt acgccggagc tgacatgaaa accaaactta
    26821 acgaactgct tgaattccct actcctttta cttacaaagt tatggggcag gcgttacctg
    26881 agctggttga tcaggtggtt gaagtggtac agcgccatgc gccaggtgac tacaccccaa
    26941 cggtaaaacc aagcagcaaa ggcaactacc actcggtatc tatcactatc aacgccactc
    27001 atatcgagca ggttgaaaca ctgtatgaag aactgggcaa aatcgatatt gtccgcatgg
    27061 ttctgtaact cgcttctccg ttacccggct cctggtcggg taactcccct ctcctcgctg
    27121 tgatatactt ttcccccact tttactcatt ctccacggag atgccgtttt gtatcaggat
    27181 aaaattcttg tccgccagct cggtcttcag ccttacgagc caatctccca ggctatgcat
    27241 gaattcaccg atacccgcga tgatagtacc cttgatgaaa tctggctggt cgagcactat
    27301 ccggtattca cccaagggca ggcaggaaag gcggagcaca ttttaatgcc gggtgatatt
    27361 ccggtgatcc agagcgatcg cggtgggcag gtgacttatc acgggccggg gcaacaggtg
    27421 atgtatgtgt tgcttaacct gaaacgccgt aaactcggtg tgcgtgaact ggtgaccttg
    27481 cttgagcaaa cagtggtgaa taccctggct gaactgggta tagaagcgca tcctcgggct
    27541 gacgcgcctg gtgtctatgt cggggaaaag aaaatttgct cactgggttt acgtattcga
    27601 cgcggttgtt cattccacgg tctggcatta aacgtcaata tggatctttc accattttta
    27661 cgtattaatc cttgtgggta tgcaggaatg gaaatggcta aaatatcaca atggaaaccc
    27721 gaagcgacga ctaataatat tgcaccacgt ttactggaaa atattttagc gctactaaac
    27781 aatcctgact tcgaatatat taccgcttaa ttccatacat caatggccca atttacactg
    27841 ggtcattact cataatacct gctaatgctt tacatctcct tcgcgtttgt atattttctt
    27901 tatcacttgc agaaatgcga tgctacgcct tgctccgtag cccacctaat tgattaactc
    27961 attgaaatga ttgaccatgg tgagcaaaag cgttccgtcc caattagaca taagattatt
    28021 gtaatttttt taaatattat ttt
//
LOCUS       DADSYM010000058        28005 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2415
            SAMN08039579-rid11723173.denovo.058, whole genome shotgun sequence.
ACCESSION   DADSYM010000058 DADSYM010000000
VERSION     DADSYM010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039579
            Sequence Read Archive: SRR6362533
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 28005)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28005)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980162.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 03:39:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,484
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 4,347
            CDSs (with protein)               :: 4,347
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 43 of 85
            Pseudo Genes (incomplete)         :: 37 of 85
            Pseudo Genes (internal stop)      :: 19 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28005
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039579-rid11723173.denovo.058"
                     /strain="LGE2415"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8302 N 95.9628 W"
                     /collection_date="2015-08-24"
     gene            complement(246..464)
                     /gene="infA"
                     /locus_tag="J1Y83_002052"
     CDS             complement(246..464)
                     /gene="infA"
                     /locus_tag="J1Y83_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001040186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HBA7630199.1"
                     /translation="MAKEDNIEMQGTVLETLPNTMFRVELENGHVVTAHISGKMRKNY
                     IRILTGDKVTVELTPYDLSKGRIVFRSR"
     gene            complement(749..1453)
                     /gene="aat"
                     /locus_tag="J1Y83_002053"
     CDS             complement(749..1453)
                     /gene="aat"
                     /locus_tag="J1Y83_002053"
                     /EC_number="2.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HBA7630200.1"
                     /translation="MRLVQLSRHSIAFPSPEGALREPNGLLALGGDLSPARLLMAYQR
                     GIFPWFSPGDPILWWSPDPRAVLWPESLHISRSMKRFHKRSPYRVTMNYAFGQVIEGC
                     ASDREEGTWITRGVVEAYHRLHELGHAHSIEVWREDELVGGMYGVAQGTLFCGESMFS
                     RMENASKTALLVFCDEFIRHGGKLIDCQVLNDHTASLGACEIPRRDYLNYLNQMRLGR
                     LPNNFWVPRCLFSPQE"
     gene            complement(1495..3216)
                     /gene="cydC"
                     /locus_tag="J1Y83_002054"
     CDS             complement(1495..3216)
                     /gene="cydC"
                     /locus_tag="J1Y83_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/glutathione ABC transporter ATP-binding
                     protein/permease CydC"
                     /protein_id="HBA7630201.1"
                     /translation="MRALLPYLALYKRHKWMLSLGIVLAIVTLLASIGLLTLSGWFLS
                     ASAVAGVAGLYSFNYMLPAAGVRGAAITRTAGRYFERLVSHDATFRVLQHLRIYTFSK
                     LLPLSPAGLARYRQGELLNRVVADVDTLDHLYLRVISPLVGAFVVIMVVTIGLSFLDF
                     TLAFTLGGIMLLTLFLMPPLFYRAGKSTGQNLTHLRGQYRQQLTAWLQGQAELTIFGA
                     SDRYRTQLENTEIQWLEAQRRQSELTALSQAIMLLIGALAVILMLWMASGGVGGNAQP
                     GALIALFVFCALAAFEALAPVTGAFQHLGQVIASAVRITDLTDQKPEVTFPDTQTRVA
                     DRVSLTLRDVQFTYPEQSQQALKGIYLQVNAGEHIAILGRTGCGKSTLLQLLTRAWDP
                     QQGEILLNDSPIASLNEAALRQTISVVPQRVHLFSATLRDNLLLASPGSSDEALEEIL
                     RRVGLEKLLEDAGLNSWLGEGGRQLSGGELRRLAIARALLHDAPLVLLDEPTEGLDAT
                     TESQILELLAEMMCEKTVLMVTHRLRGLSRFQQIIVMDNGQIIEQGTHAELLARQGRY
                     YQFKQGL"
     gene            complement(3217..4983)
                     /gene="cydD"
                     /locus_tag="J1Y83_002055"
     CDS             complement(3217..4983)
                     /gene="cydD"
                     /locus_tag="J1Y83_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/glutathione ABC transporter
                     permease/ATP-binding protein CydD"
                     /protein_id="HBA7630202.1"
                     /translation="MNKSRQKELTRWLKQQSVISQRWLNISRLLGFVSGILIIAQAWF
                     MARILQHMIMENIPREALLLPFTLLFLTFVLRAWVVWLRERVGYHAGQHIRFAIRRQV
                     LDRLQQAGPAWIQGKPAGSWATLVLEQIDDMHDYYARYLPQMALAVSVPLLIVVAIFP
                     SNWAAALILLGTAPLIPLFMALVGMGAADANRRNFLALARLSGHFLDRLRGMETLRIF
                     GRGEAEIESIRSASEDFRQRTMEVLRLAFLSSGILEFFTSLSIALVAVYFGFSYLGEL
                     DFGHYDTGVTLAAGFLALILAPEFFQPLRDLGTFYHAKAQAVGAADSLKTFMETPLAH
                     PQRGEAELASTEPVTIEAEDLFITSPEGKTLAGPLNFTLPAGQRAVLVGRSGSGKSSL
                     LNALSGFLSYQGSLRINGIELRDLSPESWRKHLSWVGQNPQLPAATLRDNVLLARPDA
                     SEQELQTALDNAWVSEFLPLLPQGVDTPVGDQAARLSVGQAQRVAVARALLNPCSLLL
                     LDEPAASLDAHSEQRVMEALNAASLRQTTLMVTHQLEDLADWDVIWVMQDGQIIEQGR
                     YAELSVAGGPFATLLAHRQEEI"
     gene            complement(5106..6071)
                     /gene="trxB"
                     /locus_tag="J1Y83_002056"
     CDS             complement(5106..6071)
                     /gene="trxB"
                     /locus_tag="J1Y83_002056"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HBA7630203.1"
                     /translation="MGTTKHSKLLILGSGPAGYTAAVYAARANLQPVLITGMEKGGQL
                     TTTTEVENWPGDPNDLTGPLLMERMHEHATKFETEIIFDHINKVDLQNRPFRLTGDSG
                     EYTCDALIIATGASARYLGLPSEEAFKGRGVSACATCDGFFYRNQKVAVIGGGNTAVE
                     EALYLSNIASEVHLIHRRDGFRAEKILIKRLMDKVENGNIILHINRTLEEVTGDQMGV
                     TGVRLRDTQNTDNIESLDVAGLFVAIGHSPNTAIFEGQLELENGYIKVQSGIHGNATQ
                     TSIPGVFAAGDVMDHIYRQAITSAGTGCMAALDAERYLDGLADAK"
     gene            6615..7109
                     /gene="lrp"
                     /locus_tag="J1Y83_002057"
     CDS             6615..7109
                     /gene="lrp"
                     /locus_tag="J1Y83_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001591745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-responsive transcriptional regulator
                     Lrp"
                     /protein_id="HBA7630204.1"
                     /translation="MVDSKKRPGKDLDRIDRNILNELQKDGRISNVELSKRVGLSPTP
                     CLERVRRLERQGFIQGYTALLNPHYLDASLLVFVEITLNRGAPDVFEQFNTAVQKLEE
                     IQECHLVSGDFDYLLKTRVPDMSAYRKLLGETLLRLPGVNDTRTYVVMEEVKQSNRLV
                     IKTR"
     gene            7244..11350
                     /gene="ftsK"
                     /locus_tag="J1Y83_002058"
     CDS             7244..11350
                     /gene="ftsK"
                     /locus_tag="J1Y83_002058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HBA7630205.1"
                     /translation="MSQEYTEDKEVTLTKLSSGRRLLEALLILIVLFAVWLMAALLSF
                     NPSDPSWSQTAWHEPIHNLGGMPGAWLADTLFFIFGVMAYTIPVIIVGGCWFAWRHQS
                     SDEYIDYFAVSLRIIGVLALILTSCGLAAINADDIWYFASGGVIGSLLSTTLQPLLHS
                     SGGTIALLCVWAAGLTLFTGWSWVTIAEKLGGWILNILTFASNRTRRDDTWVDEDEYE
                     DDEEYEDENHGKQHESRRARILRGALARRKRLAEKFINPMGRQTDAALFSGKRMDDEE
                     EITYTARGVVADPDDVLFSGNRATQPEYDEYDPLLNGAPITEPVAVAAAATTATQSWA
                     APVEPVTQTPPVASVDVPPTQPTVAWQPVPGPQTGEPVIAPAPEGYPQQSQYAQPAVQ
                     YNEPLQQPVQPQQPYYAPAAEQPVQQPYYAPAPEQSAQQPYYAPAPEQPVAGNAWQAE
                     EQQSTFAPQSTYQTEQTYQQPAAQEPLYQQPQPVEQQPVVEPEPVVEETKPTRPPLYY
                     FEEVEEKRAREREQLAAWYQPIPEPVKEPEPIKSSLKAPSVAAVPPVEAAAAVSPLAS
                     GVKKATLATGAAATVAAPVFSLANGGGPRPQVKEGIGPQLPRPKRIRVPTRRELASYG
                     IKLPSQRAAEEKAREAQRNQYDSGDQYNDDEIDAMQQDELARQFAQTQQQRYGEQYQH
                     DVPVNTEDADAAAEAELARQFAQTQQQRYSGEQPAGANPFSLDDFEFSPMKALLDDGP
                     HEPLFTPIVEPVQQPQQPVAPQQQYQQSQQPVAPQPQYQQPQQPVAPQPQYQQPQQPV
                     APQPQYQQPQQPVAPQQQYQQPQQPVTQQPQYQQPQQPVTQQPQYQQPQQPVAPQPHD
                     TLLHPLLMRNGDSRPLHKPTTPLPSLDLLTPPPSEVEPVDTFALEQMARLVEARLADF
                     RIKADVVNYSPGPVITRFELNLAPGVKAARISNLSRDLARSLSTVAVRVVEVIPGKPY
                     VGLELPNKKRQTVYLREVLDNAKFRDNPSPLTVVLGKDIAGEPVVADLAKMPHLLVAG
                     TTGSGKSVGVNAMILSMLYKAQPEDVRFIMIDPKMLELSVYEGIPHLLTEVVTDMKDA
                     ANALRWCVNEMERRYKLMSALGVRNLAGYNEKIAEADRMMRPIPDPYWKPGDSMDAQH
                     PVLKKEPYIVVLVDEFADLMMTVGKKVEELIARLAQKARAAGIHLVLATQRPSVDVIT
                     GLIKANIPTRIAFTVSSKIDSRTILDQAGAESLLGMGDMLYSGPNSTLPVRVHGAFVR
                     DQEVHAVVQDWKARGRPQYVDGITSDSESEGGVGGFDGAEELDPLFDQAVQFVTEKRK
                     ASISGVQRQFRIGYNRAARIIEQMEAQGIVSEQGHNGNREVLAPPPFD"
     gene            11509..12120
                     /gene="lolA"
                     /locus_tag="J1Y83_002059"
     CDS             11509..12120
                     /gene="lolA"
                     /locus_tag="J1Y83_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010354356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein chaperone LolA"
                     /protein_id="HBA7630206.1"
                     /translation="MKKIAITCALLSSLVASSVWADAASDLKSRLDKVSSFHASFTQK
                     VTDGSGAAVQEGQGDLWVKRPNLFNWHMTQPDESILVSDGKTLWFYNPFVEQATATWL
                     KDATGNTPFMLIARNQSSDWQQYNIKQNGDDFVLTPKASNGNLKQFTINVGRDGTIHQ
                     FSAVEQDDQRSSYQLKSQQNGAVDAAKFTFTPPQGVTVDDQRK"
     gene            12131..13474
                     /locus_tag="J1Y83_002060"
     CDS             12131..13474
                     /locus_tag="J1Y83_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HBA7630207.1"
                     /translation="MSNLSLDFSDNTFQPLAARMRPENLAQYIGQQHLLAAGKPLPRA
                     IEAGHLHSMILWGPPGTGKTTLAEVIARYANADVERISAITSGVKEIREAIERARQNR
                     NAGRRTILFVDEVHRFNKSQQDAFLPHIEDGTITFIGATTENPSFELNSALLSRARVY
                     LLKSLSTEDIEQVLTQAMEDKTRGYGGQDIVLPDETRRAIAELVNGDARRALNTLEMM
                     ADMAEVDDSGKRVLKPELLTEIAGERSARFDNKGDRFYDLISALHKSVRGSAPDAALY
                     WYARIITAGGDPLYVARRCLAIASEDVGNADPRAMQVAIAAWDCFTRVGPAEGERAIA
                     QAIVYLACAPKSNAVYTAFKAALADARERPDYDVPVHLRNAPTKLMKEMGYGQEYRYA
                     HDEANAYAAGEVYFPPEIAQTRYYFPTNRGLEGKIGEKLAWLAEQDQNSPIKRYR"
     gene            13565..14857
                     /gene="serS"
                     /locus_tag="J1Y83_002061"
     CDS             13565..14857
                     /gene="serS"
                     /locus_tag="J1Y83_002061"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000887397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HBA7630208.1"
                     /translation="MLDPNLLRNEPDAVAEKLARRGFKLDVDKLGALEERRKVLQVKT
                     ENLQAERNSRSKSIGQAKARGEDIEPLRLEVNKLGEELDAAKAELDALQAEIRDIALT
                     IPNLPADEVPVGKDENDNVEVSRWGTPREFDFEVRDHVTLGEMHSGLDFAAAVKLTGS
                     RFVVMKGQIARMHRALSQFMLDLHTEQHGYSENYVPYLVNQDTLYGTGQLPKFAGDLF
                     HTRPLEEEADTSNYALIPTAEVPLTNLVRGEIIDEDDLPIKMTAHTPCFRSEAGSYGR
                     DTRGLIRMHQFDKVEMVQIVRPEDSMAALEEMTGHAEKVLQLLGLPYRKIILCTGDMG
                     FGACKTYDLEVWIPAQNTYREISSCSNVWDFQARRMQARCRSKSDKKTRLVHTLNGSG
                     LAVGRTLVAVMENYQQADGRIEVPEVLRPYMNGLEYIG"
     gene            15096..17540
                     /gene="dmsA"
                     /locus_tag="J1Y83_002062"
     CDS             15096..17540
                     /gene="dmsA"
                     /locus_tag="J1Y83_002062"
                     /EC_number="1.8.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415414.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase subunit A"
                     /protein_id="HBA7630209.1"
                     /translation="MKTKIPDAVLAAEVSRRGLVKTTAIGGLAMASSALTLPFSRIAH
                     AVDSAIPTKSDEKVIWSACTVNCGSRCPLRMHVVNGEIKYVETDNTGDDNYDGLHQVR
                     ACLRGRSMRRRVYNPDRLKYPMKRVGARGEGKFERISWEEAYDIIATKMQRLIKEYGN
                     ESIYLNYGTGTLGGTMTRSWPPGNTLVARLMNCCGGYLNHYGDYSSAQIAEGLNYTYG
                     GWADGNSPSDIENSKLVVLFGNNPGETRMSGGGVTYYLEQARQKSNARMIIIDPRYTD
                     TGAGREDEWIPIRPGTDAALVNGLAYVMITENLVDQEFLDKYCVGYDEKTLPASAPKN
                     GHYKAYILGEGPDGVAKTPEWASQITGVPADKIIKLAREIGSTKPAFISQGWGPQRHA
                     NGEIATRAISMLAILTGNVGINGGNSGAREGSYSLPFVRMPTLENPIQTSISMFMWTD
                     AIERGPEMTALRDGVRGKDKLDVPIKMIWNYAGNCLINQHSEINRTHEILQDDKKCEL
                     IVVIDCHMTSSAKYADILLPDCTASEQMDFALDASCGNMSYVIFNDQVIKPRFECKTI
                     YEMTSELAKRLGVEQQFTEGRTQEEWMRHLYAQSREAIPELPTFEEFRKQGIFKKRDP
                     QGHHVAYKAFREDPQANPLTTPSGKIEIYSQALADIAATWELPEGDVIDPLPIYTPGF
                     ESYQDPLNKQYPLQLTGFHYKSRVHSTYGNVDVLKAACRQEMWINPLDAQKRGIHNGD
                     KVRIFNDRGEVHIEAKVTPRMMPGVVALGEGAWYDPDAKRVDKGGCINVLTTQRPSPL
                     AKGNPSHTNLVQVEKV"
     gene            17551..18168
                     /gene="dmsB"
                     /locus_tag="J1Y83_002063"
     CDS             17551..18168
                     /gene="dmsB"
                     /locus_tag="J1Y83_002063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase subunit B"
                     /protein_id="HBA7630210.1"
                     /translation="MTTQYGFFIDSSRCTGCKTCELACKDYKDLTPEVSFRRIYEYAG
                     GDWQEDNGVWHQNVFAYYLSISCNHCEDPACTKVCPSGAMHKREDGFVVVDEDVCIGC
                     RYCHMACPYGAPQYNETKGHMTKCDGCYDRVAEGKKPICVESCPLRALDFGPIDELRK
                     KHGDLAAVAPLPRAHFTKPNIVIKPNANSRPTGDTTGYLANPKEV"
     gene            18170..19033
                     /locus_tag="J1Y83_002064"
     CDS             18170..19033
                     /locus_tag="J1Y83_002064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethyl sulfoxide reductase anchor subunit"
                     /protein_id="HBA7630211.1"
                     /translation="MGSGWHEWPLMIFTVFGQCVAGGFIVLALALLKGDLRAEAQQRV
                     IACMLGLWVLMGIGFIASMLHLGSPMRAFNSLNRVGASALSNEIASGSIFFAVGGIGW
                     LLAMLKKLSPALRTLWLVVTMVLGVVFVWMMVRVYNSIDTVPTWYSIWTPMGFFLTMF
                     MGGPLLGYLLLSLAGVDGWAMRLLPAISVLALVVSGVMSVMQGAELATIHSSVQQAAA
                     LVPDYGALMSWRIVLLAVALCLWIAPQLKGYQPAVPLLSVSFILLLAGELIGRGVFYG
                     LHMTVGMAVAS"
     gene            complement(19069..19695)
                     /locus_tag="J1Y83_002065"
     CDS             complement(19069..19695)
                     /locus_tag="J1Y83_002065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HBA7630212.1"
                     /translation="MTKPYVRLDKNDAAVLLVDHQAGLLSLVRDIEPDKFKNNVLALG
                     DLAKYFNLPTILTTSFETGPNGPLVPELKAQFPDAPYIARPGNINAWDNEDFVKAVKA
                     TGKKQLIIAGVVTEVCVAFPALSAIEEGFDVFVVTDASGTFNEITRHSAWDRMSQAGA
                     QLMTWFGVACELHRDWRNDIEGLATLFSNHIPDYRNLMTSYDTLTKQK"
     gene            20009..21157
                     /locus_tag="J1Y83_002066"
     CDS             20009..21157
                     /locus_tag="J1Y83_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBA7630213.1"
                     /translation="MSTYTRPVMLLLSGLLLLTLAIAVLNTLVPLWLAQEHMSTWQVG
                     VVSSSYFTGNLVGTLLTGYVIKRIGFNRSYYLASFIFAAGCAGLGLMIGFWSWLAWRF
                     VAGIGCAMIWVVVESALMCSGTSRNRGRLLAAYMMVYYVGTFLGQLLVSKVSTELMSV
                     LPWVTGLTLAGILPLLFTRVLNQQTENHDSTSITSMLKLRQARLGVNGCIISGIVLGS
                     LYGLMPLYLNHKGVSNASIGFWMAVLVSAGILGQWPIGRLADKFGRLLVLRVQVFVVI
                     LGSIAMLSQAAMAPALFILGAAGFTLYPVAMAWACEKVEHHQLVAMNQALLLSYTVGS
                     LLGPSFTAMLMQNFSDNLLFIMIASVSFIYLLMLLRNAGHTPKPVAHV"
     gene            complement(21254..22051)
                     /gene="pflA"
                     /locus_tag="J1Y83_002067"
     CDS             complement(21254..22051)
                     /gene="pflA"
                     /locus_tag="J1Y83_002067"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015696619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase 1-activating protein"
                     /protein_id="HBA7630214.1"
                     /translation="MSNLTNCNTTENTAAAGATKSVIGRIHSFESCGTVDGPGIRFIT
                     FFQGCLMRCLYCHNRDTWDTHGGKEVTVEDLMKEVVTYRHFMNASGGGVTASGGEAIL
                     QAEFVRDWFRACKKEGIHTCLDTNGFVRRYDPVIDELLEVTDLVMLDLKQMNDEIHQN
                     LVGVSNHRTLEFAKYLANKNVKVWIRYVVVPGWSDDDDSAHRLGEFTRDMGNVEKIEL
                     LPYHELGKHKWVAMGEEYKLDGVKPPKKETMERVKGILEQYGHKVMF"
     gene            complement(22083..23078)
                     /locus_tag="J1Y83_002068"
     CDS             complement(22083..23078)
                     /locus_tag="J1Y83_002068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016158965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HBA7630215.1"
                     /translation="MSIKKLDDGRYEVDVRPQGADGKRIRRKFKTKGEAQAFERHVLV
                     NYHNKEWLEKPADRRTLTELLGRWWIYHGKSHERGDIERGRLTTIIAKFAEMGVSRAD
                     QLTKKTITDYRVVMMNDGLKPASVNRHLAIMSGMFTKLIDAGEYHSHNPFREVKRLRE
                     AVTEMAFLSSEEITRLLSMLDGDELNATLVCLSTGGRWSEVSNLKAEHIINQMVTFMK
                     TKNGKRRTIPVSQDLIKRIKTKNSGRLFNASYYKVRNALREVKPDLPDGQAVHVLRHT
                     FATHFIMNGGNIITLQRILGHSNIQQTMTYAHFAPDFLQDAVTLNPVSGMSIMRP"
     gene            complement(23172..23483)
                     /locus_tag="J1Y83_002069"
     CDS             complement(23172..23483)
                     /locus_tag="J1Y83_002069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004254765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBA7630216.1"
                     /translation="MSNTQITSQAEKLALIRESERMTRKQVAELTGINYNTYAGYEQG
                     KVKMSFDAGMKFFKPERFRKYRDWFMFDETDPAGGQIAPALAHIGQDSTTLHHSDQKT
                     G"
     gene            23588..23944
                     /locus_tag="J1Y83_002070"
     CDS             23588..23944
                     /locus_tag="J1Y83_002070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001576635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA7630217.1"
                     /translation="MSIGSEMNNDVGEKVSDLTKSKKCDIKLAAAPSDLLSKEGFALY
                     IGKTPRAVAEMAKAGKLPAFYMTDPLKPGGHAELWINRREWDKYAAQLVDEAPTEWHD
                     WKNRISYSKSRHGRAA"
     gene            24122..24622
                     /locus_tag="J1Y83_002071"
     CDS             24122..24622
                     /locus_tag="J1Y83_002071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017441883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein B"
                     /protein_id="HBA7630218.1"
                     /translation="MTVMTLNLVEKQPATMRRIIGKHLAVPRWQDTCDYYNQMMERER
                     LTVCFHAQLKQRHATMRFEEMNDVERERLVCAIDELRGAFSKRRQVGASEYAYISFLT
                     VSQRRTLFMHAGLTEKEFNQPYWRINEESCYWRDALFRALRELFSLFEYAPTILTSVK
                     PEQYLH"
     gene            24686..24910
                     /locus_tag="J1Y83_002072"
     CDS             24686..24910
                     /locus_tag="J1Y83_002072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016236090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2732 family protein"
                     /protein_id="HBA7630219.1"
                     /translation="MHTVSENQCGKYALLLQKARTEAQADAATRFSSHLDAMIRHITK
                     AELSRVEIVELLSQESEKFHNIGLSRGEVL"
     gene            24910..25209
                     /locus_tag="J1Y83_002073"
     CDS             24910..25209
                     /locus_tag="J1Y83_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001277891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA7630220.1"
                     /translation="MSCSHSVVLLNNALKIAVMENGDLSLIQLCLDKEKRDITESVIA
                     IYQNELNLLSDVVNLLVKRAVFHKQISSVDELTKLTTELASYCADVSRKLNDKRS"
     gene            25212..25436
                     /locus_tag="J1Y83_002074"
     CDS             25212..25436
                     /locus_tag="J1Y83_002074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016242005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA family transcriptional regulator"
                     /protein_id="HBA7630221.1"
                     /translation="MPDNVDFIQEQQAELLERQINAARVKHCGASALVCEECDAPIPA
                     ARRAAYPSATRCVSCQSVFEAKNKHYRRTA"
     gene            25433..25708
                     /locus_tag="J1Y83_002075"
     CDS             25433..25708
                     /locus_tag="J1Y83_002075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000027668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA7630222.1"
                     /translation="MSIRIEIGERYVVTSDSFQFILHEKKRAESGKNAGQEWLAVIGY
                     YPKLSQLVSGLMHHDILTGSAKSFADLNAQVEQLSRRCSEAFGSYGR"
     gene            25698..27971
                     /locus_tag="J1Y83_002076"
     CDS             25698..27971
                     /locus_tag="J1Y83_002076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication endonuclease"
                     /protein_id="HBA7630223.1"
                     /translation="MAVKASGRFVPPSAFAAGTGKAFTGAYAWNAPREAVGRERPLTR
                     DEMRQVQGVLSTINRLPYFLRSLFTSRYDYIRRNKSPVHGFYFLTSTFQRRLWPRIER
                     VNQRHEMNTDASLLFLAERDHYARLPGMNDKELKKFAARISSQLFMMYEELCDAWVDA
                     HGEKESLFTDEAQAHLYGHVAGAARAFNISPLYWKKYRKGQMTTRQAYSAIARLFNDE
                     WWTHQLKGQRMRWHEALLIAVGEVNKDRSPYASKHAIRDVRARRQANLEFLKSCDLEN
                     RETGERIDLISKVMGSISNPEIRRMELMNTIAGIERYAAAEGDVGMFITLTAPSKYHP
                     TRQVGKGESKTVQLNHGWNDEAFNPKDAQRYLCRIWSLMRTAFKDNDLQVYGLRVVEP
                     HHDGTPHWHMMLFCNPRQRNQIIEIMRRYALKEDGDERGAARNRFQAKHLNRGGAAGY
                     IAKYISKNIDGYALDGQLDNDTGRPLKDTAAAVTAWASTWRIPQFKTVGLPTMGAYRE
                     LRKLPRGVSIADEFDERVEAARAAADSGDFALYIRAQGGANVPRDCQTVRVARSPSDD
                     VNEYEEEVERVVGIYAPHLGARHIHITRTTDWRIVPKVPVVEPLTLKSGIAAPRSPVN
                     NCGKLTGGDTSLPAPTPSEHAAAVLNLVDDGVIEWNDPEVVRTLRGALKHGMKTPNRQ
                     QRNGSPLKPHEIAPSARLTRSERLQITRIRVDLAQNGIRPQRWELDALARGAAVLYGN
                     KKFIYATDKGWPGFSEK"
BASE COUNT         6984 a         6889 c         7324 g         6808 t
ORIGIN      
        1 ttgttgcctg acctcatggg gggcaacggg gcgctactat agggagttgg aataaaacgg
       61 tcaagaagaa ttttaatgat aattattgtt tgctcatact gtaaacaagt tgtgcagtat
      121 atctacatcg agacaagtta cggacttata cttctaaagt actccataca tatcacaaaa
      181 taaaaaggcc ggctaaaccg accttttact cattctttct cttcgcccat caggcggtaa
      241 aacaatcagc gactacggaa gacaatgcgg cctttgctca ggtcgtacgg ggtcagttca
      301 acagtcactt tgtcgcccgt caggatgcgg atgtagtttt tgcgcatttt accggagatg
      361 tgtgcagtaa ccacgtgacc gttttctaac tctacgcgga acatggtatt aggcaacgtt
      421 tcaagaacgg taccttgcat ttcaatattg tcttctttgg ccatctaatc ctctggggta
      481 tcactaccgt aatttgaacc ggcaagataa tgccgaagtt ctgtaaataa gtaaagattt
      541 gcgcgctaaa tcgcaacaaa caggttcggc acattactcc gaaaacacac ggctaagccg
      601 caccaaaagc gcaacgtata agggagcggt gagataaacg atgggcgtta cctgacgcga
      661 aaaattcctt atcggcagcg gggtaatgag cgtaaccaac tctgcgaccg caattataac
      721 actctgggga gaaatgtgcc gaaaacattc attcttgtgg tgaaaacaag catcgaggta
      781 cccagaaatt attcggcaat cgtccgaggc gcatttgatt gagataatta aggtaatccc
      841 ggcggggaat ttcgcaggca ccaagcgatg ctgtgtgatc gttaaggacc tggcagtcga
      901 taagtttacc gccatgacgg ataaattcat cacagaagac cagaagcgcc gttttggacg
      961 cattttccat ccggctgaac atggactcgc cacaaaatag tgttccctgg gccacgccgt
     1021 acataccgcc gacaagctca tcttcacgcc agacttcaat ggagtgggca tgaccgagtt
     1081 cgtgaaggcg atggtaggct tcgaccacgc cacgcgtgat ccaggttcct tcttcgcgat
     1141 cgctggcaca gccttcgatg acctgaccaa aagcgtaatt catcgtgaca cgatagggtg
     1201 agcgtttatg aaatcgcttc atactacggc tgatatgcag tgattctggc cataacaccg
     1261 cgcggggatc gggcgaccac cagaggatgg ggtcgccagg agaaaaccac gggaaaatac
     1321 cacgctgata agccattaac aggcgcgcag ggctaagatc gccccccagt gccagcaggc
     1381 cgttaggctc acgtaatgcg ccttccgggg aagggaaggc tattgaatgg cgagaaagct
     1441 gaaccaggcg catgaccgca aaactccacg caagtcggat cgttcaataa tagcttacaa
     1501 accctgctta aactggtaat aacgcccctg tctggcaagc agttctgcgt gagtaccttg
     1561 ctcaataatt tgcccgttgt ccatcactat tatttgttgg aaacgagaga gtccgcgaag
     1621 tcgatgggtg accattaaca ccgttttctc gcacatcatt tctgcaagca attcaaggat
     1681 ctgactttcg gttgtggcat ctaagccttc tgtaggttca tccagcaata ccagcggcgc
     1741 atcatgtaac agcgcacggg cgatagccag acggcgcagt tcaccaccgg agagctggcg
     1801 tccgccttca cctaaccaac tgttgagacc tgcatcctcg agcagcttct ccagaccaac
     1861 gcgacgcaag atctcctcca gagcctcatc actactacca ggcgaggcga gtaacagatt
     1921 atcacgcagc gtggcgctaa acagatgcac tcgctgggga acaacgctga tggtctgtcg
     1981 tagagccgct tcattcaggc tggctatggg actatcgtta agcaaaatct cgccctgttg
     2041 cgggtcccat gcgcgggtca gcagttgtaa cagtgttgat ttgccgcatc cggttcgccc
     2101 gagaatcgca atatgttccc cggcgtttac ctgaagataa atccctttaa gtgcctgttg
     2161 ggattgctcc ggataagtga actgaacatc ccgtaatgtc agcgaaacgc gatcggcaac
     2221 acgagtttgg gtatcaggga aggtgacttc cggtttttga tccgttaaat cagtgatccg
     2281 tacggcagag gcaatgactt gccccagatg ctgaaatgca cccgttactg gtgccagcgc
     2341 ttcaaacgcg gctaacgcgc agaagacaaa tagggcaatt aacgcgccgg gttgagcatt
     2401 gccgccaacg ccgccggacg ccatccacag catcaggatc accgctaacg cgccaatgag
     2461 cagcattatc gcttgcgaca atgcggtcag ttcagattga cggcgttgcg cttccagcca
     2521 ctgaatttct gtattctcta attgcgtgcg ataacgatcg ctggcaccaa aaatggtcag
     2581 ctcagcttgc ccttgcagcc aggccgtcag ttgttggcga tactgcccgc gaagatgagt
     2641 cagattttgc ccggtgcttt ttcccgcacg ataaaacagt ggtggcatca ggaaaagcgt
     2701 cagtaacata atgccgccca gcgtaaaggc gagggtgaaa tcaaggaaac ttaacccgat
     2761 tgtcaccacc ataatcacca caaaagcacc taccagcggc gagataacgc gcaggtaaag
     2821 atgatcgagc gtatcaacat ccgccaccac gcggttgagt aattcaccct gacgatagcg
     2881 cgccagtccg gcaggggaga ggggcagcaa tttgctgaag gtgtatatgc gcagatgctg
     2941 caacacgcgg aaagtcgcgt cgtgacttac cagacgttca aaataacgcc cggcagtacg
     3001 ggtgattgct gcgccacgca cgcccgcagc gggtagcata tagttgaagc tgtacagtcc
     3061 ggcaaccccc gcaaccgctg aggcggagag gaaccagccc gaaagtgtca acagaccgat
     3121 actggcgagc agcgtgacaa ttgccagcac gataccaaga cttaacatcc atttatgacg
     3181 tttatacagt gccagatagg gtagcaaagc gcgcatttaa atctcctcct gacgatgggc
     3241 cagtaatgtg gcgaatgggc cgccagccac acttaattcc gcgtaacgtc cttgctcaat
     3301 aatctgacca tcctgcatta cccaaatgac atcccagtca gcaagatctt ctaactggtg
     3361 ggtgaccatt aacgttgtct ggcgcagaga ggcggcattc agcgcctcca ttacgcgctg
     3421 ttcactgtga gcatcaaggc tggcagcggg ttcatccaac agtaatagcg aacagggatt
     3481 tagtaacgca cgggccaccg ccacgcgctg cgcctgcccc acggaaaggc gggcagcctg
     3541 gtcgccaaca ggcgtatcaa cgccttgtgg caggagcggt agaaactcac tgacccaggc
     3601 gttatccagc gctgtttgta actcttgttc gctggcatca ggtcgcgcca gtagtacgtt
     3661 atcccgtaat gttgctgccg gtaattgtgg gttttgccca acccaggaga gatgtttacg
     3721 ccatgattcc ggtgataaat cgcgtaattc tatcccgttg attcgtagcg atccctgata
     3781 tgagagaaaa ccagaaagcg cgttcaacag tgaactttta cctgaaccgc tgcgaccaac
     3841 caacactgct cgttggcctg ctggcaaagt aaaattcagc ggtccggcca gcgttttacc
     3901 ttccggcgac gtgataaaca gatcctcggc ttcaatggtc accggctcgg tcgatgctaa
     3961 ttccgcttca ccgcgctgcg gatgggcgag cggggtttcc ataaacgttt tcaaactgtc
     4021 agctgcacca acagcctggg ctttagcatg ataaaacgta ccgagatcgc gtaatggctg
     4081 gaaaaactct ggcgcaagga tcagggccag aaaacccgca gccagcgtca caccagtatc
     4141 gtagtgacca aaatccagct cgccgaggta ggaaaaacca aagtagaccg ccaccagagc
     4201 aatcgacagc gaggtaaaaa attcgagaat gccggaggat aaaaacgcca gccgtagcac
     4261 ttccattgtc cgttggcgga aatcttccga agcagaacga atactttcaa tttcagcttc
     4321 accacgacca aaaatacgca atgtttccat gccgcgcagg cgatcgagga aatgcccact
     4381 taagcgagca agagcgagaa agttacgtcg gttagcatcg gcagccccca ttccaaccag
     4441 cgccataaat aacggaatta gcggtgcagt gcccagcaga atgagcgccg cggcccagtt
     4501 agaggggaag atagccacca caatcagcag cggcaccgac actgccagcg ccatttgcgg
     4561 caggtagcgt gcatagtaat catgcatatc gtcaatttgc tcgagtacca gcgtcgccca
     4621 gctccccgct ggtttaccct gaatccacgc tggccctgct tgttgcagac ggtcgagaac
     4681 ctgacggcgg atggcaaagc ggatatgctg cccggcgtga taacccaccc gttcgcgtaa
     4741 ccagaccacc catgcgcgca gtacaaaggt cagaaacagt aacgtaaagg gaagcagcag
     4801 ggcttcacgg ggaatattct ccataatcat atgttgcaga attcgcgcca tgaaccaggc
     4861 ctgggcaatg atcaatatgc cgctcacaaa gcccagcaga cgagaaatat tcagccaacg
     4921 ttgggagatg acgctttgct gttttaacca gcgggttaat tctttttgac gggatttatt
     4981 cattgcacgc ttagcaggtg agttatcaga attatttgca gagcaatgtt acaacgggga
     5041 aaaaataaag gcgacccata gtcgcatggt gtcgccttct ttacttttgt tactgatttg
     5101 taaaattatt ttgcgtcagc taaaccgtcg aggtagcgtt ccgcatcaag agctgccatg
     5161 cagcctgtac cagcagatgt aatggcctgg cgataaatgt gatccatcac gtcgcctgcg
     5221 gcaaagacgc cagggatgct ggtctgggtg gcattaccat gaatacccga ctgtactttg
     5281 atgtagccgt tttccagttc cagctgccct tcgaaaatgg cagtattcgg gctgtgaccg
     5341 atagcaacaa acagaccggc aacgtcgagt gactcgatat tatcggtgtt ttgcgtatcg
     5401 cgcagacgaa cgccagtgac gcccatctga tcgccggtca cttcttccag cgtacggttg
     5461 atgtgcagaa taatgttgcc gttctccact ttatccatca gacgcttaat gaggattttt
     5521 tccgcacgga aaccatctcg gcggtgaatc agatgaactt ccgaagcgat gttagacaga
     5581 tacagcgcct cttcaaccgc ggtattgccg ccgccgatga ccgcaacttt ctggttgcga
     5641 tagaagaaac cgtcgcaggt tgcacaagca gaaactccac ggcctttaaa ggcttcttca
     5701 gagggcaggc cgagatagcg tgcagaagct ccggtggcaa taatcagcgc gtcgcaagtg
     5761 tattcgccgc tatcgccagt cagacggaac ggacgattct gcaaatcaac tttgttgatg
     5821 tgatcaaaga tgatttcagt ttcaaatttg gtggcatgtt cgtgcatgcg ctccattaat
     5881 aacggaccgg tcagatcgtt tggatcgcca ggccagtttt ccacttccgt ggtggtggtc
     5941 agttggccgc ctttttccat gccggtaatc agcacaggtt gcaggttggc gcgcgccgcg
     6001 tagacagcag cggtgtatcc cgccgggcct gaacccagga taagcagttt actgtgtttg
     6061 gtcgtgccca tgagatcccc atagttgttg gcagacaatg ggcaggattg tagggaattt
     6121 acagacgtaa aaaaagagta tgacgatttt gttaacaatt tgtgcaatcg gcagcatcga
     6181 taagcaggtc aaattctccc gtcattatca cctctgctac ttaaatttcc cgctttataa
     6241 gccgattaaa tgatgaataa acgcccctgt taatgaatat ctggcatgtt gtactaaaaa
     6301 tcgatgtttt gctttgacaa tcccctggtg ttttgcgaaa acattcgagg aagaaaaaaa
     6361 cagtattctt atatgcgcat aaccatgcat gtaaatacca tgtttaccgt gctagtgaaa
     6421 tctacgtatg gcgtggacag acgccattcg tgatgtcgat agctgccaca aggcaacggt
     6481 cttctcaccg tagacccagg cattgcgcgc cgtgaatctt catgatttcg gtctatcgtg
     6541 acgggtagcg actctgaaca gtgatgtttc agggtcagac aggagtaggg aaggaataca
     6601 gagagacaat aataatggta gatagcaaga agcgccctgg caaagatctc gaccgtatcg
     6661 atcgtaacat tcttaatgag ttgcaaaagg atgggcgtat ttctaacgtc gagctttcta
     6721 aacgtgtggg actttcccca acgccgtgcc ttgagcgtgt gcgtcggctg gaaagacaag
     6781 ggtttattca gggctatacg gcgctgctga acccccatta tctggatgca tcacttctgg
     6841 tattcgttga gattactctg aatcgtggcg caccggatgt gtttgaacaa ttcaataccg
     6901 ctgtacaaaa acttgaagaa attcaggagt gtcatttagt atccggtgat ttcgactacc
     6961 tgttgaaaac acgcgtgccg gatatgtcag cttaccgtaa gttactgggg gaaaccctgc
     7021 tgcgtctgcc tggcgtcaat gacacacgga catacgttgt tatggaagaa gtcaagcaga
     7081 gtaatcgtct ggttattaag acgcgctaac acggaacagg tgcaaaatcg gcgtattttg
     7141 attacactcc tgttaatcca tacagcaaca gtactggggt aacctggtac tgttgtccgt
     7201 tttagcatcg ggcaggaaaa gcctgtaacc tggagagcct ttcttgagcc aggaatacac
     7261 tgaagacaaa gaagtcacat tgacaaagtt aagtagcggc cgccgccttc tggaggcgtt
     7321 gctgatcctt attgtcctgt ttgccgtctg gttgatggct gccttactaa gctttaaccc
     7381 ttcggacccc agctggtcgc aaacggcctg gcatgaacct atccataatt taggtgggat
     7441 gcccggtgcg tggttggcag atacgctgtt ctttattttt ggcgtgatgg cttacaccat
     7501 tcccgtcatt attgtcggcg gttgttggtt tgcctggcgt catcagtcca gcgacgaata
     7561 cattgattat tttgccgttt cgctacgcat cattggcgtt ttggcgctca tccttacctc
     7621 ctgtggtctg gcggcaatca acgctgacga tatctggtat tttgcctccg gtggcgtcat
     7681 tggcagctta ctaagcacta cgctacaacc actgctacac agtagcgggg gaactattgc
     7741 gctgctctgc gtttgggcag cgggcctgac gctgttcacc ggttggtcat gggtgaccat
     7801 tgctgaaaaa ctcggcggct ggattttaaa tattcttacc ttcgccagta atcgtacccg
     7861 tcgcgatgat acctgggtcg atgaagatga gtatgaagac gacgaagagt atgaagatga
     7921 aaatcacggc aaacagcatg aatcacgccg tgcccgtatt cttcgcggcg cgctagcgcg
     7981 tcgtaaacgg ttggcggaaa aattcattaa tccgatgggg cggcaaacag atgctgcgct
     8041 gttctccggt aagcggatgg atgatgaaga agagattacc tacactgcac gcggtgtggt
     8101 tgccgaccct gacgacgtcc tgttttcggg caatcgtgca acgcagccag aatatgacga
     8161 atacgatcca ttattaaacg gtgcgccaat taccgaacct gttgctgtag cagctgctgc
     8221 taccacggcg acacaaagct gggctgcacc ggttgaacct gtgactcaga cgccgccagt
     8281 tgcctctgtt gatgttccac ctacgcaacc tacagtagcc tggcagcctg taccggggcc
     8341 gcaaacggga gaaccggtta ttgcacctgc accggaaggt tacccacagc agtcacaata
     8401 tgcgcagcct gcagtgcaat ataatgagcc gctgcaacaa cctgtacagc cacagcagcc
     8461 gtattatgcg cctgcagctg aacagcctgt gcaacaacct tactacgctc ctgcgccgga
     8521 acagtctgcg caacagccgt attacgcccc tgcgccagaa caaccggtgg caggtaacgc
     8581 ctggcaagcc gaagagcagc aatccacttt tgctccacag tctacatacc agacggagca
     8641 aacttatcag cagccagccg ctcaggagcc gttgtaccaa cagccgcaac ccgttgaaca
     8701 gcagcctgtt gtggagcctg aacccgttgt agaagagaca aaacccacgc gtccgccgct
     8761 ttactatttt gaagaagtgg aagagaagcg agcccgtgaa cgtgaacaac ttgcggcctg
     8821 gtatcaaccg attccagaac cggttaaaga accggaaccg atcaaatctt cgctgaaagc
     8881 accttctgtt gcagcagtac ctccagtaga agccgctgcc gctgtttctc cgctggcatc
     8941 tggtgtgaaa aaagcgacac tggcgacggg ggctgccgca accgttgccg cgccagtctt
     9001 cagtctggca aatggcggtg gaccgcgtcc gcaggtcaaa gaggggattg gcccgcagtt
     9061 gccacgaccg aaacgcatcc gcgtgccaac tcgtcgtgaa ctggcgtctt acggtattaa
     9121 gctgccctca cagcgtgcgg cagaagaaaa agcccgtgaa gcccagcgta atcagtacga
     9181 ttctggcgat cagtacaacg atgatgaaat cgatgcgatg cagcaggatg aactggcacg
     9241 tcagttcgcc cagacgcagc agcaacgcta tggcgaacag tatcaacatg atgtgcccgt
     9301 gaacacagaa gatgcagatg ctgcggcaga ggcagaactg gctcgtcagt ttgctcaaac
     9361 tcaacaacaa cgttattccg gcgaacaacc ggctggggcg aatccgttct cgctggatga
     9421 ttttgaattt tcgccaatga aagcgttgct ggatgatggt ccacacgaac cgctgtttac
     9481 gccaattgtt gaacctgtac agcagccgca acaaccggta gcaccgcagc agcaatatca
     9541 gcagtcgcaa caaccggttg caccgcagcc gcagtatcag cagccgcaac aaccggttgc
     9601 accgcagccg cagtatcagc agccgcaaca accagttgcg ccacagccgc aatatcaaca
     9661 gccgcaacaa ccggttgcac cgcagcagca atatcagcag ccacaacagc cggttacaca
     9721 gcagccgcag tatcagcagc cgcaacagcc ggttacacag cagccgcagt atcagcagcc
     9781 gcaacagccg gttgcgccgc agccgcatga taccctgctt catccgctgt tgatgcgcaa
     9841 tggtgacagc cgtccgttgc ataaaccgac gacgccgctg ccttctctgg atttgctgac
     9901 gccgccgccg agcgaagtgg agccggtaga tacctttgcg cttgaacaaa tggcgcgtct
     9961 ggtggaagcg cgtctggctg atttccgtat taaagccgat gtcgtcaatt attctccggg
    10021 gccggttatc actcgctttg aattgaacct ggcaccgggc gtaaaagcag cgcgcatttc
    10081 taacttgtca cgtgaccttg cccgttcact ttcgacggtg gcggtgcgtg tcgttgaagt
    10141 tattcctggc aaaccctatg taggtctgga gttaccgaat aaaaaacgac aaaccgttta
    10201 tctgcgcgag gttttggata acgccaaatt ccgcgataat ccgtcgccat taaccgtggt
    10261 gctgggtaaa gatatcgccg gtgagccggt ggttgccgat ctggcgaaaa tgccgcactt
    10321 gttggttgcg gggactaccg gttccggtaa gtctgtcggg gtgaacgcga tgatcctgag
    10381 catgctttat aaagcacagc cagaagatgt gcgtttcatc atgatcgacc cgaaaatgct
    10441 ggagctttcg gtttatgaag gcattccgca cctgttaacg gaagttgtta ctgatatgaa
    10501 agatgccgcc aacgcgctgc gctggtgtgt taacgagatg gagcgtcgtt ataaactgat
    10561 gtctgcgctg ggtgtgcgta atctggcggg ttataacgaa aaaattgctg aagccgatcg
    10621 catgatgcgt ccgattccag acccgtactg gaagccgggt gatagtatgg atgcgcagca
    10681 tccggtgctg aaaaaagaac catacattgt ggtgttggtg gacgaatttg ccgacctgat
    10741 gatgacggta ggtaaaaaag tggaagagct gatagcacgt ctggcgcaaa aagcccgtgc
    10801 cgcgggtatc cacctcgtac tggcaacgca gcgtccatcg gttgatgtta ttactggtct
    10861 gattaaagcg aatattccga cccgtatcgc ctttaccgta tccagtaaga ttgactcacg
    10921 taccattctt gatcaggctg gtgcggaatc actgttgggt atgggggata tgctttactc
    10981 tgggccgaac tccacgttgc cggtacgtgt tcatggtgct tttgttcgcg atcaggaagt
    11041 tcatgccgtg gtgcaggact ggaaagcgcg tggtcgccca cagtatgttg atggcatcac
    11101 ctccgacagt gaaagcgaag gtggcgtggg cggtttcgat ggcgctgaag aactggatcc
    11161 gttgttcgat caggcggtgc agtttgtcac tgaaaaacgc aaagcgtcaa tttctggcgt
    11221 acagcgtcag ttccgcattg gttataaccg tgcagcgcgt attatcgaac agatggaagc
    11281 gcaggggatt gtcagcgaac aggggcacaa cggtaatcgt gaagtactgg ccccaccgcc
    11341 atttgactaa ttaatgcaac gtaggccgga taagacgcga tagcgtcgca tccggtattc
    11401 tataaactga aaattcagta ttttcttctt tcctcaagct gattattagc ctggaataga
    11461 gagtagaggg aactcccgat tgggagtgac gtaatttgag gaataatgat gaaaaaaatt
    11521 gccatcacct gtgcattact ctcaagctta gtagcaagca gcgtttgggc tgatgccgca
    11581 agcgatctta aaagccgctt ggataaagtc agcagtttcc acgccagctt cacacaaaaa
    11641 gtgactgacg gtagcggcgc ggcggtgcag gaaggtcagg gcgatctgtg ggtgaaacgt
    11701 ccaaacttat tcaactggca tatgactcaa cctgacgaaa gcattctggt ttctgacggt
    11761 aaaacactgt ggttctataa cccgttcgtt gagcaagcta cggcaacctg gctgaaagat
    11821 gccaccggta atacgccgtt tatgctgatt gcccgcaacc agtccagcga ctggcagcag
    11881 tacaatatca aacagaatgg cgatgacttt gtcctgacgc cgaaagccag caatggcaat
    11941 ctgaagcagt tcaccattaa cgtgggacgt gatggcacaa tccatcagtt tagcgcggtg
    12001 gagcaggacg atcagcgcag cagttatcag ctgaaatccc agcaaaatgg ggctgtggat
    12061 gcagcgaaat ttaccttcac cccgccgcaa ggcgtcacgg tagatgatca acgtaagtag
    12121 aggcacctga gtgagcaatc tgtcgctcga tttttcggat aatacttttc aacctctggc
    12181 cgcgcgtatg cggccagaaa atttagcaca gtatatcggc cagcaacatt tgctggctgc
    12241 ggggaagccg ttgccgcgcg ctatcgaagc cgggcatctg cattctatga tcctctgggg
    12301 gccgccgggt accggcaaaa caaccctcgc tgaagtgatt gcccgctatg cgaacgctga
    12361 tgtggaacgt atttctgcca tcacctctgg tgtgaaagag attcgcgagg cgatcgagcg
    12421 cgctcggcaa aaccgcaatg caggtcgccg cactattctt tttgttgacg aagttcaccg
    12481 tttcaacaaa agccagcagg atgcatttct gccacatatt gaagacggca ccatcacttt
    12541 tattggcgca accactgaaa acccgtcgtt tgagcttaat tcggcactgc tttcccgtgc
    12601 ccgtgtctat ctgttgaaat ccctgagtac agaggatatt gagcaagtac taactcaggc
    12661 gatggaagac aaaacccgtg gctatggtgg tcaggatatt gttctgccag atgaaacacg
    12721 acgcgccatt gctgaactgg tgaatggcga cgcgcgccgg gcgttaaata cgctggaaat
    12781 gatggcggat atggccgaag tcgatgatag cggtaagcgg gtcctgaagc ctgaattact
    12841 gaccgaaatc gccggtgaac gtagcgcccg ctttgataac aaaggagatc gcttttacga
    12901 tctgatttcc gcactgcata agtcggtacg tggtagcgca cccgatgcgg cgctgtactg
    12961 gtatgcgcga attattaccg ctggtggcga tccgttatat gtcgcgcgtc gctgtctggc
    13021 gattgcgtct gaagacgtcg gtaatgccga tccacgggcg atgcaggtgg caattgcggc
    13081 ctgggattgc tttactcgcg ttggcccggc ggaaggtgaa cgcgccattg ctcaggcgat
    13141 tgtttacctg gcttgcgcgc caaaaagcaa cgctgtctac actgcgttta aagccgcgct
    13201 ggcggatgca cgcgaacgtc cggattatga cgtgccggtt catttgcgta atgcgccgac
    13261 gaaattaatg aaggaaatgg gctacgggca ggaatatcgt tacgctcatg atgaagcaaa
    13321 cgcttatgct gccggtgagg tttatttccc gccggaaata gcacaaacac gctattattt
    13381 cccgacaaac aggggccttg aaggcaagat tggcgaaaag ctcgcctggc tggctgaaca
    13441 ggatcaaaat agccccataa aacgctaccg ttaatgttat cgttgcggta atgttgttaa
    13501 tgtatccctg tggtcgcagg ctgtggccac atctcccatt taattcgata agcacaggat
    13561 aagcatgctc gatcccaatc tgctgcgtaa tgagccagac gcagtcgctg aaaaactggc
    13621 acgccggggc tttaagctgg atgtagataa gctgggcgct cttgaggagc gtcgtaaagt
    13681 attgcaggtc aaaacggaaa acctgcaagc ggagcgtaac tcccgatcga aatccattgg
    13741 ccaggcgaaa gcgcgcgggg aagatatcga gcctttacgt ctggaagtga acaaactggg
    13801 cgaagagctg gatgcagcaa aagccgagct ggatgcttta caggctgaaa ttcgcgatat
    13861 cgcgctaacc atccctaacc tgcctgcaga tgaagtgccg gtaggtaaag acgaaaatga
    13921 caacgttgaa gtcagtcgct ggggcacccc gcgtgagttt gactttgaag ttcgtgacca
    13981 tgtgacgctg ggtgaaatgc actctggcct cgactttgct gccgcagtta agttgactgg
    14041 ttcccgcttt gtggtaatga aagggcagat tgctcgcatg caccgcgcac tgtcgcagtt
    14101 tatgctggat ctgcataccg aacagcatgg ctacagtgag aactatgttc cgtacctggt
    14161 taaccaggac acgctgtacg gtacgggtca gctgccgaaa tttgctggcg atctgttcca
    14221 tactcgtccg ctggaagaag aagcagacac cagtaactat gcgctgatcc caacggcaga
    14281 agttccgctg accaacctgg tacgcggtga aatcatcgat gaagatgatc tgccaattaa
    14341 gatgaccgcc cacaccccat gtttccgttc tgaagctggt tcatatggtc gtgacactcg
    14401 tggtctgatc cgtatgcacc agttcgacaa agttgaaatg gtgcagatcg tgcgcccgga
    14461 agactcaatg gcggcactgg aagagatgac cggtcatgcg gaaaaagtcc tgcagctgct
    14521 gggtctgccg taccgcaaaa tcatcctttg taccggcgac atgggctttg gtgcttgcaa
    14581 aacttacgac ctggaagtat ggatcccggc acagaacacc taccgcgaga tctcttcctg
    14641 ctccaacgtc tgggatttcc aggcgcgtcg tatgcaggca cgttgccgca gcaagtcgga
    14701 caagaaaact cgtctggttc ataccctgaa cggttctggt cttgctgttg gtcgtacgct
    14761 ggttgcagta atggaaaact atcagcaggc tgatggtcgt attgaagtac cagaagttct
    14821 gcgtccgtat atgaacggac tggaatatat tggctaatac ccaatttttc tgaatctaaa
    14881 aagcgcctgc gggcgctttt tttgtctccc tttgataccg aacaataatt actcctcact
    14941 tacaagtaat actactttcg agtgaaaatc tacctatctc tttgattttc aaattattcg
    15001 atatatacaa gcctatatag cgatctgcta tagaaataat tacacaatac ggtttgttac
    15061 tggaatcaat cgtgagcaag cttgagtgag ccattatgaa aacgaaaatc cctgatgcgg
    15121 tattggctgc tgaggtgagt cgccgtggtt tggtaaaaac gacagcgatc ggcggcctgg
    15181 caatggccag cagcgcatta acattacctt ttagtcggat tgcgcacgct gtcgatagcg
    15241 ctattccaac aaaatcagac gaaaaggtta tctggagcgc ctgtacagtt aactgtggta
    15301 gtcgctgccc gctacgtatg cacgtagtga acggtgaaat caaatatgtc gaaacggaca
    15361 ataccggcga tgacaattac gacggcctgc accaggttcg cgcctgtctg cgtgggcgtt
    15421 ccatgcgtcg ccgtgtctac aatccggacc gcctgaaata tccgatgaaa cgagtcgggg
    15481 cgcgcggtga aggcaaattc gagcgcatta gctgggaaga agcctacgac atcatcgcga
    15541 ccaaaatgca gcgcctgatc aaagagtacg gcaacgagtc tatctatctg aactatggca
    15601 ccggtacgct gggcggcacc atgacccgct cgtggccgcc gggaaatacc ctggtcgcgc
    15661 ggctaatgaa ctgctgcggc ggttatctga accattatgg cgactactcc tccgcgcaaa
    15721 ttgcggaagg tctgaactat acctacggcg gctgggcaga tggtaacagc ccgtcggata
    15781 tcgaaaacag taaactggta gtgctgtttg gtaataaccc tggcgaaacg cgaatgagtg
    15841 gcggtggggt gacttactat cttgaacagg cgcgccagaa atctaatgct cgcatgatca
    15901 tcatcgatcc gcgctatacc gacaccggtg ccgggcgtga agatgagtgg atccctattc
    15961 gtccgggaac agatgccgca ctggttaacg gtctggcgta tgtcatgatc accgaaaacc
    16021 tggtagatca ggaattcctc gataaatatt gcgttggcta cgacgagaaa accctgccag
    16081 ccagtgcgcc gaaaaatggt cactataaag cttatattct gggtgaagga cccgatggcg
    16141 tggctaaaac accggaatgg gcctcgcaaa tcaccggtgt tccggcagac aaaatcatca
    16201 aactggctcg tgaaatcggc agtaccaaac cggcgtttat cagccaggga tggggcccgc
    16261 agcgtcacgc taacggtgaa atcgcaaccc gtgctatctc gatgctggcg attctgaccg
    16321 gtaacgttgg tattaacgga ggcaacagcg gcgcgcgcga aggttcatac agcttaccgt
    16381 ttgtccgtat gccgactctc gaaaacccga tccagaccag catttctatg tttatgtgga
    16441 ccgatgccat tgaacgtggc ccggaaatga cggcgctgcg tgatggtgtg cgcgggaaag
    16501 ataagctgga tgtgccgatc aaaatgatct ggaactatgc cggtaactgc ctgattaatc
    16561 agcattctga aatcaaccgt acccatgaaa tccttcagga tgataagaag tgcgagctga
    16621 ttgtggttat cgactgccac atgacctcat cggcgaaata tgctgacatc ctgctgcctg
    16681 actgcaccgc ttccgaacag atggactttg cactggatgc atcctgcggg aatatgtctt
    16741 acgttatttt caatgatcag gtcattaaac cgcgctttga atgtaagacc atctatgaga
    16801 tgaccagcga actggcaaaa cgtcttggcg tcgaacaaca gtttactgaa ggtcgtactc
    16861 aggaagagtg gatgcggcat ctgtatgccc agtcgcggga agcgattcct gaactgccaa
    16921 cgtttgaaga gttccgtaag caggggatct ttaaaaagcg cgatccgcaa gggcatcacg
    16981 ttgcttacaa agccttccgt gaagatccgc aggctaatcc attgaccacg ccatcgggca
    17041 aaattgagat ctattcacag gcgctggctg acattgccgc tacctgggaa ttgccagaag
    17101 gtgatgtgat cgatccactg ccgatctata cgccgggctt tgaaagttat caggatccgc
    17161 tgaacaaaca gtatccgctg cagcttacgg gtttccacta taaatctcgc gttcactcaa
    17221 cttacggcaa cgttgatgtg ctgaaagcag cttgccgtca ggaaatgtgg atcaacccgc
    17281 ttgatgcgca aaaacgcggt atccacaacg gcgataaagt caggatcttt aacgatcgtg
    17341 gtgaggtcca tattgaggcg aaagtgacgc cacgaatgat gccgggtgtg gtcgcactgg
    17401 gtgaaggtgc ctggtatgac ccggatgcaa aacgtgtcga taagggcggt tgtattaacg
    17461 tactgaccac tcaacgtccg tctcctctcg ctaaggggaa tccgtcacat acaaaccttg
    17521 ttcaggttga aaaggtgtaa ggagtaaccg atgacaaccc agtatggatt ttttattgat
    17581 tccagccgtt gcaccggttg caaaacctgc gagctggctt gtaaagacta caaagatttg
    17641 acgccagaag tcagcttccg ccgcatttat gaatatgctg gtggcgactg gcaggaagat
    17701 aacggtgtct ggcaccagaa cgtgtttgcc tactatctgt cgatttcatg taaccactgc
    17761 gaagatccgg cttgtactaa agtctgcccg agcggtgcga tgcataaacg tgaagatggt
    17821 tttgttgtgg tcgatgaaga tgtatgcatt ggctgccgtt actgtcatat ggcttgcccg
    17881 tatggcgcac cgcaatataa cgaaacgaaa ggccatatga ccaaatgcga tggttgttat
    17941 gaccgtgttg cggagggaaa aaaaccgatc tgcgtagagt cctgcccgct gcgggcgctg
    18001 gatttcggcc ctatcgacga gctgcgtaaa aaacatggcg atctggcggc cgttgcgccg
    18061 ttgccgcgag ctcactttac caaaccgaat attgtgatca aacccaatgc caacagccgc
    18121 ccaaccgggg ataccactgg ctatctggca aacccgaagg aggtgtgaga tgggaagtgg
    18181 atggcatgaa tggccgctga tgatcttcac ggtcttcggg caatgtgtag cgggtggttt
    18241 tatcgttctg gctttggcgc tgctcaaagg cgacctgcga gcagaagccc agcagcgtgt
    18301 tatcgcctgc atgttgggtt tatgggtgct gatgggcatt ggctttatcg cctctatgct
    18361 tcatcttggt tcaccgatgc gcgcttttaa ctcgctcaat cgggtagggg cttcagcact
    18421 cagtaacgaa attgccagcg gttcgatctt ttttgctgta ggcggcatcg gctggttgct
    18481 ggcaatgctg aaaaagcttt caccggcatt gcgtacgctt tggctggtgg tgacgatggt
    18541 tcttggcgta gtctttgtct ggatgatggt gcgtgtgtat aacagcatcg atacagtacc
    18601 cacctggtac agcatctgga caccgatggg cttcttcctg acgatgttta tgggcggccc
    18661 gttactgggt tacctgttgc tgagtctggc tggtgtcgat ggctgggcga tgcgtctgct
    18721 gccagccatt tctgttctgg cactggtagt gagtggcgtg atgtcagtga tgcagggcgc
    18781 agagctggcg actattcata gctctgtgca gcaggccgca gcgctggtgc cggactatgg
    18841 tgcgctgatg tcctggcgga tcgtgctttt ggccgttgcc ctgtgcttgt ggattgcacc
    18901 acagctaaaa ggttatcagc ctgcggttcc gctactttct gtatcattca ttctgcttct
    18961 ggcaggggaa ttaatcggtc gtggcgtatt ctatggtttg catatgaccg tggggatggc
    19021 tgtcgcaagt taatcagtaa caaccggggt ttcagccccg gttcgctttt atttctgctt
    19081 cgttaacgtg tcataactgg tcatcaagtt acgataatca ggaatgtggt tagagaacaa
    19141 cgtcgccagt ccttcaatat cattacgcca gtcgcgatgc agctcgcacg ccacgccgaa
    19201 ccatgtcatc aattgcgcac cagcttgcga catgcgatcc catgccgaat gccgggtaat
    19261 ttcattaaag gtccctgacg catcagtcac gacaaagacg tcaaaccctt cttcaatggc
    19321 tgaaagcgcc gggaatgcca cgcaaacttc ggttaccaca ccggcaataa ttaactgttt
    19381 tttacctgtc gctttgacag cttttacaaa atcttcgtta tcccaggcgt taatatttcc
    19441 cgggcgagca atgtaaggtg catcggggaa ttgtgctttc agttctggaa ctaatgggcc
    19501 gttaggaccg gtttcaaaac tggtggtaag aatagttggt aagttgaaat acttagccag
    19561 gtcacccagc gccagcacgt tatttttaaa cttatcgggt tcgatatccc gtacaaggga
    19621 aagtaaacca gcctggtgat caacaagcaa aacggcagca tcatttttat caagacgaac
    19681 atacggtttg gtcatcctct tctcctttcg agagtcagtg gtacgtctaa atcggtcaga
    19741 cgcgatgcat tgctctgaaa gcatagacgg gaaatatgag tttgctgtga ccatgaaatt
    19801 tttcgactga acgaagcgta tgaaatttgt gttagatcaa taaaaacaat cagatgagat
    19861 taatcgtctc ttcagcattg gcgttatgaa tgagtaatat aaatcgataa taccgccgct
    19921 gcgatatgcg cgtattttca cccattgaca atgtttttgg cggtggcatg atgcgcatga
    19981 aatttgaact tcctcacggt tttaattcat gtccacgtat acccggcctg tcatgctttt
    20041 gctgtctggc ctgcttttgt tgactctggc gatcgcggtg ttaaatacac tcgtgccgct
    20101 ttggctcgcc caggaacaca tgtctacatg gcaggtaggc gttgtcagct catcctattt
    20161 taccggcaac cttgtcggta cattgctgac agggtatgtc attaaacgca ttggctttaa
    20221 ccgtagctat tatctggcct ccttcatttt tgccgctggc tgtgccggcc ttggcctgat
    20281 gattggattc tggagttggt tggcgtggcg ttttgtcgct ggcatcggct gtgccatgat
    20341 ttgggtggtt gttgagagtg cgctgatgtg cagtgggacg tcgcgtaacc gcgggcggtt
    20401 gcttgctgcg tatatgatgg tttattacgt cggaacgttt ttaggccagt tactggtcag
    20461 caaagtctca accgagttga tgagtgtatt gccgtgggtt acaggtttga cgttggcagg
    20521 aatcttaccg ctgttgttta cgcgtgtcct gaatcagcag actgaaaacc atgattcgac
    20581 gtcaattacg tcaatgctaa aactccgtca ggcgcggctt ggcgtgaatg gctgcattat
    20641 ctcaggaatc gtgctgggat ctctatatgg cctgatgccg ctgtacctca atcacaaagg
    20701 ggtgagcaat gccagcattg gtttctggat ggcggtactg gtcagtgcgg gtatcctcgg
    20761 gcaatggccg attggacgtc tggcggataa gtttggtcga ctgctggtgt tgcgtgttca
    20821 ggtctttgtc gtcattctcg gcagtattgc gatgcttagc caggcggcga tggccccagc
    20881 gttattcatc ctcggtgccg ctggctttac gctatatccg gtggcgatgg cctgggcttg
    20941 tgagaaagtt gaacatcatc aactggtggc gatgaaccag gccttactgt tgagctatac
    21001 cgtgggaagc ctgcttggcc cgtcatttac cgctatgcta atgcagaatt tctccgataa
    21061 tttattattt atcatgatcg ccagcgtatc gtttatctat ttgctgatgc tgcttcgtaa
    21121 cgccggtcat acgccgaaac ccgtggctca cgtgtaaatg aattcaagca gagtgtgaac
    21181 ttactgtttc acactctgct tttctgtgca caaaaaagcg gtaactcaat aaagttgccg
    21241 ctttacgggg aaattagaac atgaccttat gaccgtactg ctcaagaatg cctttcacgc
    21301 gttccatggt ctctttcttc ggtggtttaa caccatcgag tttgtattct tcacccatcg
    21361 ccacccattt gtgtttgccc agttcgtggt aggggagaag ttcgattttc tcaacgttgc
    21421 ccatatcacg ggtaaattca ccaaggcgat gcgctgaatc gtcatcgtca gaccagcctg
    21481 ggacaacaac atagcggatc cacaccttca catttttgtt cgccagatat ttagcgaact
    21541 ccagcgtgcg gtggttggaa actccaacca gattttggtg gatctcgtcg ttcatctgtt
    21601 tgagatcgag cattaccagg tcggttactt ccagcagttc atcaatcacc ggatcgtaac
    21661 gacgaacaaa accgttggtg tccagacagg tatgaatgcc ttcttttttg caggcgcgga
    21721 accagtcacg aacaaactca gcttgtagga ttgcctcacc gccggatgcg gtaacgccgc
    21781 cgccggaagc gttcataaag tggcgatagg tcaccacttc cttcatcaaa tcttcaacgg
    21841 taacttcttt accgccatgc gtatcccagg tgtcgcggtt atgacaatac aggcagcgca
    21901 tcaggcagcc ctggaaaaag gtgataaagc gaatacccgg gccgtctacg gttccacagg
    21961 attcaaagga gtgaatgcga ccaataactg acttggtagc ccccgccgca gcggtatttt
    22021 ctgttgtatt acaattagtt aaattactca tatcgcttca attggctcta gttaactctg
    22081 gtttatggac gcattatgga cattcctgac accgggttaa gagtcacagc atcttgtaag
    22141 aaatccggtg caaagtgtgc gtaggtcata gtttgctgaa tgttagaatg acccaggatg
    22201 cgctgcaatg tgattatgtt acctccattc attataaaat gtgtggcaaa tgtatgcctc
    22261 aaaacatgca ctgcctgtcc gtcaggtaaa tcgggtttta cttccctgag agcgttgcgc
    22321 actttgtagt aactggcatt aaaaagcctg cctgaatttt tggtcttgat ccgtttaatc
    22381 aggtcctgcg aaacgggaat tgtcctgcgc tttccgtttt tagttttcat aaacgtaacc
    22441 atctggttaa tgatgtgttc agcttttaaa ttggacactt cactccagcg tccaccagta
    22501 gaaaggcaga ccagagtcgc atttaattca tcaccatcga gcatggataa cagccgtgta
    22561 atctcttcac tggacaaaaa agccatttct gtaacagctt cacgtaatcg cttaacctca
    22621 cggaacgggt tgtgagaatg gtattcaccg gcgtcaatta acttggtgaa catcccgctc
    22681 attattgcca gatgccgatt tacgctggct ggttttagac catcgttcat cattacaacg
    22741 cgataatcag ttatcgtttt ctttgttagc tggtcagctc tggacactcc catttctgca
    22801 aatttggcga ttattgtcgt caaacgcccc cgttcaatat ctccacgctc atgtgatttt
    22861 ccgtgatata tccaccatct gcctaacaac tctgtaagag ttcggcggtc ggctggcttc
    22921 tccagccact ctttgttgtg gtagttaacc agtacatgac gttcgaatgc ttgagcttca
    22981 cctttagttt taaatttccg cctgatacgt tttccatctg caccctgcgg tctgacgtcc
    23041 acttcataac gaccatcatc gagcttttta atagacataa agccctccga tgatgctgtt
    23101 tacttctact acttgaaaat taatgcaatt ttctttcgta catttactgc acacatatgc
    23161 tgaataaatc gtcagccagt cttttggtct gagtggtgca aggttgttga gtcttgccca
    23221 atgtgcgcga gcgccggggc tatttgtccg ccagcgggat cagtttcatc aaacatgaac
    23281 cagtcacggt acttgcgaaa tctttctggc ttgaaaaatt tcatgcctgc gtcaaaagac
    23341 atctttactt ttccctgctc atatccagca taggtgttgt agttaattcc agttaattca
    23401 gcaacttgct tccttgtcat tctttctgac tcccgaataa gtgcgagttt ctctgcttga
    23461 gatgtgattt gtgtatttga catgaattgt cgtatctcgt aatttatgtt gcatgcgaca
    23521 cgccagaaca acgcagagcg gcttcaaata gctctggttg aatggcacca aagttgagga
    23581 tatcaaaatg agtattggat cagaaatgaa taacgatgtt ggagaaaaag tatctgatct
    23641 cacaaaaagt aaaaaatgtg acatcaaact tgcagccgca ccgtcggatt tgctctcgaa
    23701 agagggattt gctctttaca tcggtaagac gcctcgtgct gttgctgaaa tggcgaaagc
    23761 aggcaagtta ccagcctttt atatgacgga cccattaaag ccgggcggtc atgctgagtt
    23821 atggattaat cgccgtgagt gggacaagta cgcagcccaa ctagttgatg aagctccgac
    23881 agaatggcat gactggaaaa atcgcattag ttacagcaaa tcaagacatg gccgtgcggc
    23941 ttaaggtgga aaggatgaac gagcctcgtt gtattgctca gttattgcgt aacgaaagcc
    24001 ccagggcgat tgacttcacc attacccacg gtaaggggcg taagggaatc attatccgca
    24061 ccaaaaaaca gagtccgtta aaaaaggctc tgacctttct gaaaagccgg agggtatgga
    24121 aatgacagtg atgacgctca atctagttga aaaacaacca gcaactatgc gccggataat
    24181 tggcaagcat cttgccgtcc ctcgctggca ggatacatgt gattattata atcagatgat
    24241 ggagcgcgaa cggctaacgg tttgctttca tgcgcagtta aaacaacgtc acgcaacgat
    24301 gcgttttgaa gaaatgaacg acgtcgaacg tgaacggctg gtttgtgcaa ttgatgaact
    24361 gcgtggggca ttctcaaaac gccgtcaggt cggtgcaagt gagtatgcat atattagttt
    24421 tttaacagtc agtcagcgtc gtactttatt tatgcatgcg ggattgacag aaaaagaatt
    24481 caatcagccg tactggcgaa ttaatgaaga atcatgttac tggcgtgatg ctttattccg
    24541 tgcattacgt gaattattca gtctgtttga gtatgcaccg acaattctga cgtcggtaaa
    24601 accagagcaa tatctgcatt aagtaattaa ccagagtttt taacgcactt aatcgtgcgg
    24661 ggcttctttt tgcctggaga aagtcatgca tacagtttct gaaaatcagt gcggtaaata
    24721 cgcattactg ctgcaaaagg ccagaaccga agcacaggcc gacgcagcga cgcgcttttc
    24781 ttctcatctt gacgccatga ttcgccacat cacaaaggcg gagttatccc gcgtggagat
    24841 agtcgagctg ctcagtcagg agtcggaaaa atttcacaat atcggattgt ctcgcgggga
    24901 ggtgctttga tgtcctgttc tcattcagtt gtattactga ataacgcctt aaaaatcgcc
    24961 gttatggaaa atggcgattt gtctcttatt caactttgtc ttgataaaga aaaacgcgac
    25021 ataactgaat ctgttatcgc gatttatcag aatgaattaa acctcctgtc tgatgtggtc
    25081 aatttacttg ttaaacgcgc tgtattccac aagcaaattt cctccgtgga tgaactgaca
    25141 aagttaacga cagaacttgc cagttattgc gctgatgtat ccaggaaact taacgataaa
    25201 aggagctgat aatgccggac aacgtagatt ttattcagga acaacaggct gaattactgg
    25261 agcgccagat taacgcggca agggtaaaac attgcggtgc ttctgcgctg gtttgtgaag
    25321 agtgtgacgc gccaatacct gctgcccgtc gtgctgctta tccctcagcc acgcgttgtg
    25381 tttcctgtca gtcagtcttt gaagcaaaaa acaaacatta ccggagaacg gcatgagtat
    25441 tcgtattgaa attggcgaac gttatgtcgt taccagtgac agctttcagt ttattctcca
    25501 cgagaaaaag agagctgaaa gcggtaaaaa cgccggtcag gaatggctgg cggtgattgg
    25561 ttattacccg aaattaagcc agctcgtttc cggcctgatg catcacgata ttctgaccgg
    25621 aagcgcaaag tcttttgctg atttaaacgc gcaggttgag caactcagca ggcgttgttc
    25681 agaggctttt ggctcatatg gccgttaaag cctccgggcg ttttgtccct ccgtcagcat
    25741 ttgctgcagg caccggtaag gcgtttaccg gtgcttatgc atggaacgcg ccacgcgagg
    25801 ctgtcgggcg cgaaaggccc cttacacgtg acgagatgcg tcaggtgcaa ggtgttttat
    25861 ccacgattaa ccgcctgcct tattttttgc gctcgctgtt tacttcacgc tatgactaca
    25921 tccggcgcaa taaaagcccg gtgcacgggt tttatttcct cacatccact tttcagcgtc
    25981 gtttatggcc gcgcattgag cgcgttaatc agcgccatga aatgaacacc gacgcgtcgt
    26041 tgctgtttct ggcagagcgt gaccactatg cgcgcctgcc tggaatgaat gacaaggagc
    26101 tgaaaaagtt tgccgcccgt atttcatcgc agcttttcat gatgtatgag gaactctgcg
    26161 atgcctgggt ggatgcgcat ggcgaaaaag aatcactgtt tacggatgag gcgcaggctc
    26221 acctctatgg tcatgttgct ggcgcagcac gtgctttcaa tatttcccct ctctactgga
    26281 aaaaataccg taaaggacag atgaccacga ggcaggcata ttctgccatt gcccgtctgt
    26341 ttaacgatga gtggtggact catcagctta aaggccagcg tatgcgctgg catgaggcgt
    26401 tactgattgc tgtcggggag gtcaataaag accgttctcc ttatgccagt aaacacgcca
    26461 ttcgtgatgt gcgtgcacgc cgccaggcaa atctggaatt tcttaaatcg tgtgaccttg
    26521 aaaacaggga aaccggcgag cgcatcgacc ttatcagtaa ggtgatgggc agtatttcta
    26581 atcctgaaat tcgccggatg gagctgatga acaccattgc cggtattgag cgttacgccg
    26641 cagcagaggg tgatgtgggg atgtttatca cgctgaccgc gccgtcaaag tatcacccga
    26701 cacgtcaggt cggaaaaggc gaaagtaaaa ccgtccagct taatcacggc tggaatgatg
    26761 aggcatttaa tccaaaggat gcgcagcgtt atctctgccg tatctggagc ctgatgcgca
    26821 cggcattcaa ggataatgat ttacaggtct acggtttgcg tgtcgtcgag ccacaccacg
    26881 acggaacgcc gcactggcat atgatgcttt tttgtaatcc acgccagcgt aaccagatta
    26941 tcgaaatcat gcgtcgctat gcgctcaaag aggatggtga cgaaagagga gccgcgcgaa
    27001 accgttttca ggcaaagcac cttaaccggg gcggtgctgc gggatatatc gcgaaataca
    27061 tctcaaaaaa catcgatggc tatgcactgg atggtcagct cgataacgat accggcaggc
    27121 cgctgaaaga cacagcggcg gctgttaccg catgggcgtc aacgtggcgc ataccacaat
    27181 ttaaaacggt tggcctgccg acaatggggg cttaccgtga actacgcaaa ttgcctcgcg
    27241 gcgtcagcat tgctgatgag tttgacgagc gcgtcgaggc tgcacgcgcc gccgcagaca
    27301 gtggtgattt tgcgttgtat atccgcgcgc agggtggggc aaatgtcccg cgcgattgtc
    27361 agactgtcag ggtcgcccgt agcccgtcgg atgacgttaa cgagtacgag gaagaggtcg
    27421 agagagtggt cggcatttac gcgccgcatc tcggcgcgcg tcatattcat atcaccagaa
    27481 cgacggactg gcgcattgtg ccgaaagttc ctgtcgttga gcctttgact ttaaaaagcg
    27541 gcatcgccgc gcctcggagt cctgtcaata actgtggaaa gctcaccggt ggtgatactt
    27601 cgttaccggc tcccacacct tctgagcacg ccgcagcagt gctaaatctg gttgatgacg
    27661 gtgttatcga atggaatgac ccggaggtcg tgaggacgct caggggggca ttaaaacacg
    27721 gcatgaaaac accaaatcgt cagcaaagaa acggaagccc gttaaaacca catgaaatag
    27781 caccgtcggc cagactgacc cggtcggaac gattgcaaat tacccgtatc cgcgttgacc
    27841 ttgctcagaa cggtatcagg ccgcagcgat gggagctcga cgcgctggct cgtggggcag
    27901 cggtgcttta cggtaacaaa aagttcattt atgcgaccga taagggatgg ccagggtttt
    27961 ctgagaagta acctgtttta tttctaaata ttaaacagca aatca
//
LOCUS       DADSYM010000059        26771 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2415
            SAMN08039579-rid11723173.denovo.059, whole genome shotgun sequence.
ACCESSION   DADSYM010000059 DADSYM010000000
VERSION     DADSYM010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039579
            Sequence Read Archive: SRR6362533
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 26771)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26771)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980162.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 03:39:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,484
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 4,347
            CDSs (with protein)               :: 4,347
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 43 of 85
            Pseudo Genes (incomplete)         :: 37 of 85
            Pseudo Genes (internal stop)      :: 19 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26771
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039579-rid11723173.denovo.059"
                     /strain="LGE2415"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8302 N 95.9628 W"
                     /collection_date="2015-08-24"
     gene            complement(310..1710)
                     /gene="asnS"
                     /locus_tag="J1Y83_002077"
     CDS             complement(310..1710)
                     /gene="asnS"
                     /locus_tag="J1Y83_002077"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HBA7630224.1"
                     /translation="MSVVPVADVLQGRVAVDSEVTVRGWVRTRRDSKAGISFLAVYDG
                     SCFDPVQAVINNSLPNYNEDVLRLTTGCSVIVTGKVVASPGQGQQFEIQASKVEVAGW
                     VEDPDTYPMAAKRHSIEYLREVAHLRPRTNLIGAVARVRHTLAQALHRFFNEQGFFWV
                     STPLITASDTEGAGEMFRVSTLDLENLPRNDQGKVDFDKDFFGKESFLTVSGQLNGET
                     YACALSKIYTFGPTFRAENSNTSRHLAEFWMLEPEVAFANLNDIAGLAEAMLKYVFKA
                     VLEERADDMKFFAERVDKDAVSRLERFIEADFAQVDYTDAVTILENCGRKFENPVYWG
                     VDLSSEHERYLAEEHFKAPVVVKNYPKDIKAFYMRLNEDGKTVAAMDVLAPGIGEIIG
                     GSQREERLDVLDERMLEMGLNKEDYWWYRDLRRYGTVPHSGFGLGFERLIAYVTGVQN
                     VRDVIPFPRTPRNASF"
     gene            complement(1879..3081)
                     /gene="pncB"
                     /locus_tag="J1Y83_002078"
     CDS             complement(1879..3081)
                     /gene="pncB"
                     /locus_tag="J1Y83_002078"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HBA7630225.1"
                     /translation="MTQFASPVLHSLLDTDAYKLHMQQAVFHHYYDVHVAAEFRCRGD
                     DLLGIYADAIREQVQAMQHLRLQDDEYQWLSALPFFKADYLNWLREFRFNPEQVTVSN
                     DNGKLDIRLSGPWREVILWEVPLLAVISEMVHRYRSPQADVAQALDTLENKLVDFSAL
                     TAGLDMSRFHLMDFGTRRRFSREVQETIVKRLQQESWFVGTSNYDLARRLSLTPMGTQ
                     AHEWFQAHQQISPDLANSQRAALAAWLEEYPDQLGIALTDCITMDAFLRDFGVEFASR
                     YQGLRHDSGDPVEWGEKAIAHYEKLGIDPQSKTLVFSDNLDLRKAVELYRHFSSRVQL
                     SFGIGTRLTCDIPQVKPLNIVIKLVECNGKPVAKLSDSPGKTICHDKAFVRALRKAFD
                     LPHIKKAS"
     gene            3347..5959
                     /gene="pepN"
                     /locus_tag="J1Y83_002079"
     CDS             3347..5959
                     /gene="pepN"
                     /locus_tag="J1Y83_002079"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000193787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase N"
                     /protein_id="HBA7630226.1"
                     /translation="MTQQPQAKYRHDYRAPDYQITDIDLTFDLDAQKTVVTAVSQAVR
                     HGASDVPLRLNGEDLKLVSVYINDEPWTAWKEEEGALVISNLPERFTLKIVNEISPAA
                     NTALEGLYQSGDALCTQCEAEGFRHITYYLDRPDVLARFTTKIIADKTKYPFLLSNGN
                     RVAQGELENGRHWVQWQDPFPKPCYLFALVAGDFDVLRDTFTTRSGREVALELYVDRG
                     NLDRAPWAMTSLKNSMKWDEERFGLEYDLDIYMIVAVDFFNMGAMENKGLNIFNSKYV
                     LARTDTATDKDYLDIERVIGHEYFHNWTGNRVTCRDWFQLSLKEGLTVFRDQEFSSDL
                     GSRAVNRINNVRTMRGLQFAEDASPMAHPIRPDMVIEMNNFYTLTVYEKGAEVIRMIH
                     TLLGEENFQKGMQLYFERHDGSAATCDDFVQAMEDASNVDLSHFRRWYSQSGTPVVTV
                     KDDYNPETEQYTLTISQRTPATPDQAEKQPLHIPFAIELYDNEGKVIPLQKGGHPVNS
                     VLNVTQAEQTFVFDNVYFQPVPALLCEFSAPVKLEYKWSDQQLTFLMRHARNDFSRWD
                     AAQSLLATYIKLNVARHQQGQPLSLPVHVADAFRAVLLDEKIDPALAAEILTLPSVNE
                     MAELFDIIDPIAIAEVREALTRTLATELADELLAIYNANYQSEYRVEHEDIAKRTLRN
                     ACLRFLAFGETHLADVLVSKQYHEANNMTDALAALSAAVAAQLPCRDALMQEYDDKWH
                     QDGLVMDKWFILQATSPAANVLETVRGLLQHRSFTMSNPNRIRSLIGAFAGSNPAAFH
                     AEDGSGYQFLVEMLTDLNSRNPQVASRLIEPLIRLKRYDAKRQEKMRAALEQLKGLEN
                     LSGDLYEKITKALA"
     gene            complement(6154..6921)
                     /gene="ssuB"
                     /locus_tag="J1Y83_002080"
     CDS             complement(6154..6921)
                     /gene="ssuB"
                     /locus_tag="J1Y83_002080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonates ABC transporter ATP-binding
                     protein"
                     /protein_id="HBA7630227.1"
                     /translation="MNTARLNQGTPLLLNAVSKHYAENIVLNQLDLHIPAGQFVAVVG
                     RSGGGKSTLLRLLAGLETPTAGDVLAGTTPLAEIQEDTRMMFQDARLLPWKSVIDNVG
                     LGLKGQWRDAARRALAAVGLENRAGEWPAALSGGQKQRVALARALIHRPGLLLLDEPL
                     GALDALTRLEMQDLIVSLWQEHGFTVLLVTHDVSEAVAMADRVLLIEEGKIGLDLTVD
                     IPRPRRLGSVRLAELEAEVLQRVMQRGHSEQPIRRHG"
     gene            complement(6918..7709)
                     /gene="ssuC"
                     /locus_tag="J1Y83_002081"
     CDS             complement(6918..7709)
                     /gene="ssuC"
                     /locus_tag="J1Y83_002081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415454.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonate ABC transporter permease
                     SsuC"
                     /protein_id="HBA7630228.1"
                     /translation="MATPVKKWLLRVAPWFLPVGIVAVWQLASSVGWLSTRILPSPEG
                     VVMAFWTLSASGELWQHLAISSWRALIGFSIGGSLGLILGLISGLSRWGERLLDTSIQ
                     MLRNVPHLALIPLVILWFGIDESAKIFLVALGTLFPIYINTWHGIRNIDRGLVEMARS
                     YGLSGIPLFIHVILPGALPSIMVGVRFALGLMWLTLIVAETISANSGIGYLAMNAREF
                     LQTDVVVVAIILYALLGKLADVSAQLLERLWLRWNPAYHLKEATV"
     gene            complement(7721..8866)
                     /gene="ssuD"
                     /locus_tag="J1Y83_002082"
     CDS             complement(7721..8866)
                     /gene="ssuD"
                     /locus_tag="J1Y83_002082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMNH2-dependent alkanesulfonate monooxygenase"
                     /protein_id="HBA7630229.1"
                     /translation="MSLNMFWFLPTHGDGHYLGTEEGSRPVDHGYLQQIAQAADRLGY
                     TGVLIPTGRSCEDAWLVAASMIPVTQRLKFLVALRPSVTSPTVAARQAATLDRLSNGR
                     ALFNLVTGSDPQELAGDGVFLDHSERYEASAEFTQVWRRLLLGETVDFNGKHIHVRGA
                     KLLFPPIQQPYPPLYFGGSSDVAQELAAEQVDLYLTWGEPPELVKEKIEHVRAKAAAH
                     GRKIRFGIRLHVIVRETNDEAWQAAERLISHLDDETIAKAQAAFARTDSVGQQRMAAL
                     HNGKRDNLEISPNLWAGVGLVRGGAGTALVGDGPTVAARINEYAALGIDSFVLSGYPH
                     LEEAYRVGELLFPHLDVAIPEIPQPQPLNPQGEAVANDFIPRNVAQS"
     gene            complement(8863..9822)
                     /locus_tag="J1Y83_002083"
     CDS             complement(8863..9822)
                     /locus_tag="J1Y83_002083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415456.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfonate ABC transporter substrate-binding
                     protein"
                     /protein_id="HBA7630230.1"
                     /translation="MRKIIKLALAGLLSVSTLAVAAESSPEALRIGYQKGSIGMVLAK
                     SHQLLEKRYPQTKISWVEFPAGPQMLEALNVGSIDLGSTGDIPPIFAQAAGADLVYVG
                     VEPPKPKAEVILVAENSPIKTVADLKGHKVAFQKGSSSHNLLLRALRQAGLKFTDIQP
                     TYLTPADARAAFQQGNVDAWAIWDPYYSAALLQGGVRVLKDGTDLNQTGSFYLAARPY
                     AEKNGAFIQGVLTTFSEADALTRSQREQSIALLAKTMGLPAPVIASYLDHRPPTTIKP
                     LSAEVAALQQHTADLFYENRLVPKKVDIRQRIWQPTQLEGKQL"
     gene            complement(9815..10390)
                     /gene="ssuE"
                     /locus_tag="J1Y83_002084"
     CDS             complement(9815..10390)
                     /gene="ssuE"
                     /locus_tag="J1Y83_002084"
                     /EC_number="1.5.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HBA7630231.1"
                     /translation="MRVITLAGSPRFPSRSSSLLEYVREKLNGLDVEVYHWNLQNFVP
                     EDLLYARFDSPALKTFTEQLQQADGLIVATPVYKAAYSGALKTLLDLLPERALQGKVV
                     LPLATGGTVAHLLAVDYALKPVLSTLKAQEILHGVFADDSQVIDYQHKPKFTPNLQTR
                     LDTALETFWQALHRRDVQVPDLLSLRGNAHA"
     gene            10627..11637
                     /gene="pyrD"
                     /locus_tag="J1Y83_002085"
     CDS             10627..11637
                     /gene="pyrD"
                     /locus_tag="J1Y83_002085"
                     /EC_number="1.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent dihydroorotate dehydrogenase"
                     /protein_id="HBA7630232.1"
                     /translation="MYYPFVRKALFQLDPERAHEFTFQQLRRITGTPFEALVRQKVPA
                     KPVNCMGLTFKNPLGLAAGLDKDGECIDALGAMGFGSIEIGTVTPRPQPGNDKPRLFR
                     LVDAEGLINRMGFNNLGVDNLVENVKKAHYDGVLGINIGKNKDTPVEQGKDDYLICME
                     KIYAYAGYIAINISSPNTPGLRTLQYGEALDDLLTAIKNKQNDLQVMHHKYVPIAVKI
                     APDLSEEELIQVADSLVRHNIDGVIATNTTLDRSLVQGMKNCDQTGGLSGRPLQLKST
                     EIIRRLSQELNGRLPIIGVGGIDSVIAAREKIAAGASLVQIYSGFIFKGPPLIKEIVT
                     HI"
     gene            11811..12353
                     /gene="zapC"
                     /locus_tag="J1Y83_002086"
     CDS             11811..12353
                     /gene="zapC"
                     /locus_tag="J1Y83_002086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415466.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapC"
                     /protein_id="HBA7630233.1"
                     /translation="MRIKPDDNWRWYYDEEHDRMMLDLANGMLFRSRFARKMLTPDAF
                     SPAGFCVDDAALYFSFEEKCRDFNLSKEQKAELVLNALVAIRYLKPQMPKSWHFVSHG
                     EMWMPMPGDAAYVWLSDTHEQVNLLVVESGENAALCLLAQPCVVIAGRAMQLGDAIKI
                     MNDRLKPQVNVDSFSLEQAV"
     gene            complement(12350..13459)
                     /gene="ycbX"
                     /locus_tag="J1Y83_002087"
     CDS             complement(12350..13459)
                     /gene="ycbX"
                     /locus_tag="J1Y83_002087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-N-hydroxylaminopurine resistance protein YcbX"
                     /protein_id="HBA7630234.1"
                     /translation="MATLTRLFIHPVKSMRGIGLTHTLADVSGLAFDRIFMITEPDGT
                     FITARQFPLMVRFTPSPVHDGLHLTAPDGSSAYVRFADFATQDAPTEVWGTHFTARIA
                     PDAINKWLSGFFSREVQLRWVGPQMTRRVKRHNTVPLSFADGYPYLLANEASLRDLQQ
                     RCPASVKMEQFRPNLVVSGASAWEEDSWKVIRIGDVVFDVVKPCSRCIFTTVSPEKGQ
                     KHPAGEPLKTLQSFRTAQDNGDVDFGQNLIARNSGVIRVGDEVEILATAPAKIYGAGA
                     ADDTANITQQPDANVDIDWQGQAFRGNNQQVLLEQLENQGIRIPYSCRAGICGSCRVQ
                     LLEGEVTPLKKSAIGDDGTILCCSCVPKTALKLAR"
     gene            13557..15665
                     /gene="rlmKL"
                     /locus_tag="J1Y83_002088"
     CDS             13557..15665
                     /gene="rlmKL"
                     /locus_tag="J1Y83_002088"
                     /EC_number="2.1.1.173"
                     /EC_number="2.1.1.264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 23S rRNA
                     (guanine(2069)-N(7))-methyltransferase RlmK/23S rRNA
                     (guanine(2445)-N(2))-methyltransferase RlmL"
                     /protein_id="HBA7630235.1"
                     /translation="MNSLFASTARGLEELLKTELENLGAVECQVVQGGVHFKGDTRLV
                     YQSLMWSRLASRIMLPLGECKVYSDLDLYLGVQAINWTEMFNPGATFAVHFSGLNDTI
                     RNSQYGAMKVKDAIVDAFTRKNLPRPNVDRDAPDIRVNVWLHKETASIALDLSGDGLH
                     LRGYRDRAGIAPIKETLAAAIVMRSGWQPGTPLLDPMCGSGTLLIEAAMLATDRAPGL
                     HRGRWGFSGWTQHDEAIWQEVKAEAQTRARKGLAEYSSHFYGSDSDARVIQRARTNAR
                     LAGIGELITFEVNDVAQLANPLPKGPYGTVLSNPPYGERLDSEPALIALHSLLGRIMK
                     NQFGGWNLSLFSASPDLLSCLQLRADKQYKAKNGPLDCVQKNYHVAESTPDSKPVMAA
                     EDYANRLRKNLKKFEKWARQEGIECYRLYDADLPEYNVAVDRYADWVVVQEYAPPKTI
                     DAHKARQRLFDIIAATISVLGIAPNKLVLKTRERQKGKNQYQKLGEKGEFLEVTEYNA
                     HLWVNLTDYLDTGLFLDHRIARRMLGQMSKGKDFLNLFSYTGSATVHAGLGGARSTTT
                     VDMSRTYLEWAERNLRLNGLTGRAHRLIQADCLAWLREANEQFDLIFIDPPTFSNSKR
                     MEDAFDVQRDHLALMKDLKRLLRAGGTIMFSNNKRGFRMDLDGLAKLGLKAQEITQKT
                     LSQDFARNRQIHNCWLITAA"
     gene            15677..17584
                     /locus_tag="J1Y83_002089"
     CDS             15677..17584
                     /locus_tag="J1Y83_002089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBA7630236.1"
                     /translation="MSLISMHGAWLSFSDAPLLDNAELHIEDNERVCLVGRNGAGKST
                     LMKILNREQGLDDGRIIYEQDLIVARLQQDPPRNVEGSVYDFVAEGIEEQAEYLKRYH
                     DISRLVMNDPSEKNLNELAKVQEQLDHHNLWQLENRINEVLAQLGLDPNVALSSLSGG
                     WLRKAALGRALVSNPRVLLLDEPTNHLDIETIDWLEGFLKTFNGTIIFISHDRSFIRN
                     MATRIVDLDRGKLVTYPGNYDQYLLEKEEALRVEELQNAEFDRKLAQEEVWIRQGIKA
                     RRTRNEGRVRALKAMRRERGERREVMGTAKMQVEEASRSGKIVFEMEDVCYQVDGKQL
                     VKDFSAQVLRGDKIALIGPNGCGKTTLLKLMLGQLQADSGRIHVGTKLEVAYFDQHRA
                     ELDPDKTVMDNLAEGKQEVMVNGKPRHVLGYLQDFLFHPKRAMTPVRALSGGERNRLL
                     LARLFLKPSNLLILDEPTNDLDVETLELLEELIDSYQGTVLLVSHDRQFVDNTVTECW
                     IFEGGGKIGRYVGGYHDARGQQEQYVALKQPAVKKSEEAAAPKAETVKRSSSKLSYKL
                     QRELELLPQLLEDLEAKLEALQTQVADASFFSQPHEQTQKVLADMAAAEQELEQAFER
                     WEYLEALKNGG"
     gene            17714..18967
                     /gene="pqiA"
                     /locus_tag="J1Y83_002090"
     CDS             17714..18967
                     /gene="pqiA"
                     /locus_tag="J1Y83_002090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity-associated transporter
                     subunit PqiA"
                     /protein_id="HBA7630237.1"
                     /translation="MCEHHHAAKHILCSQCDMLVALPRLEHGQKAACPRCGTTLTVAW
                     DAPRQRPTAYALAALFMLLLSNLFPFVNMNVAGVTSEITLLEIPGVLFSEDYASLGTF
                     FLLFVQLVPAFCLITILLLVNRAELPVRLKEQLARVLFQLKTWGMAEIFLAGVLVSFV
                     KLMAYGSIGVGSSFLPWCLFCVLQLRAFQCVDRRWLWDDIAPMPELRQPLKPGVTGIR
                     QGLRSCSCCTAILPADEPVCPRCSTKGYVRRRNSLQWTLALLVTSIMLYLPANILPIM
                     VTDLLGSKMPSTILAGVILLWSEGSYPVAAVIFLASIMVPTLKMIAIAWLCWDVKGHG
                     KRDSERMHLIYEVVEFVGRWSMIDVFVIAVLSALVRMGGLMSIYPAMGALMFALVVIM
                     TMFSAMTFDPRLSWDRQPESEHEES"
     gene            18972..20612
                     /gene="pqiB"
                     /locus_tag="J1Y83_002091"
     CDS             18972..20612
                     /gene="pqiB"
                     /locus_tag="J1Y83_002091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intermembrane transport protein PqiB"
                     /protein_id="HBA7630238.1"
                     /translation="MESNNGEAKIQKVKNWSPVWIFPIVTALIGAWVLFYHYSHQGPE
                     VTLITANAEGIEGGKTTIKSRSVDVGVVESATLADDLTHVEIKARLNSGMEKLLHKDT
                     VFWVVKPQIGREGISGLGTLLSGVYIELQPGAKGSKMDKYDLLDSPPLAPPDAKGIRV
                     LLDSKKAGQLSPGDPVLFRGYRVGSVETSTFDTQKRNISYQLFINAPYDRLVTSNVRF
                     WKDSGIAVDLTSAGMRVEMGSLTTLLSGGVSFDVPEGLDLGQPVAPKTAFVLYDDQKS
                     IQDSLYTDHIDYLMFFKDSVRGLQPGAPVEFRGIRLGTVSKVPFFAPNMRQTFNDDYR
                     IPVLIRIEPERLKMQLGENADVVEHLGELLKRGLRGSLKTGNLVTGALYVDLDFYPNT
                     PAITGIREFNGYQIIPTVSGGLAQIQQRLMEALDKINKLPLNPMIEQATSTLSESQRT
                     MKNLQTTLDSMNKILASQSMQQLPTDMQSTLRELNRSMQGFQPGSAAYNKMVADMQRL
                     DQVLRELQPVLKTLNEKSNALVFEAKDKKDPEPKRAKQ"
     gene            20609..21172
                     /gene="pqiC"
                     /locus_tag="J1Y83_002092"
     CDS             20609..21172
                     /gene="pqiC"
                     /locus_tag="J1Y83_002092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309063.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity-associated transporter
                     subunit PqiC"
                     /protein_id="HBA7630239.1"
                     /translation="MKKWLVTIAALWLAGCSSGEINKNYYQLPVVQSGTQSTASQGNR
                     LLWVEQVAVPDYLAGNGVVYQTSDVKYVIANNNLWASPLDQQLRNTLVANLSTQLPGW
                     VVASQPLGSAQDTLNVTVTEFNGRYDGKVIVSGEWLLNHQGQLIKRPFRLEGVQTQDG
                     YDEMVKVLASVWSQEAASIAQEIKRLP"
     gene            21428..21595
                     /gene="rmf"
                     /locus_tag="J1Y83_002093"
     CDS             21428..21595
                     /gene="rmf"
                     /locus_tag="J1Y83_002093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome modulation factor"
                     /protein_id="HBA7630240.1"
                     /translation="MKRQKRDRLERAHQRGYQAGIAGRSKEMCPYQTLNQRSQWLGGW
                     REAMADRVVMA"
     gene            complement(21665..22183)
                     /gene="fabA"
                     /locus_tag="J1Y83_002094"
     CDS             complement(21665..22183)
                     /gene="fabA"
                     /locus_tag="J1Y83_002094"
                     /EC_number="4.2.1.59"
                     /EC_number="5.3.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014915875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-hydroxydecanoyl-ACP
                     dehydratase/trans-2-decenoyl-ACP isomerase"
                     /protein_id="HBA7630241.1"
                     /translation="MVDKRESYTKEDLLASGRGELFGAKGPQLPAPNMLMMDRVVKMT
                     ETGGNFDKGYVEAELDINPDLWFFGCHFIGDPVMPGCLGLDAMWQLVGFYLGWLGGEG
                     KGRALGVGEVKFTGQVLPTAKKVTYRIHFKRIVNRRLIMGLADGEVLVDGRLIYTAND
                     LKVGLFQDTSAF"
     gene            complement(22252..24012)
                     /locus_tag="J1Y83_002095"
     CDS             complement(22252..24012)
                     /locus_tag="J1Y83_002095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lon protease family protein"
                     /protein_id="HBA7630242.1"
                     /translation="MTITKLAWRDLVPDTDSYQEIFAQPHLIDENDPLFSDTQPRLQF
                     ALEQLLHTRASSSFMLAKAPEESEYLNLIADAARTLQSDAGQLVGGHYEVSGHTIRLR
                     HAVSADDNFATLTQVVAADWVEAEQLFGCLRQFNGDITLQPGLVHQANGGILIISLRT
                     LLAQPLLWMRLKNIVNRERFDWVAFDESRPLPVSVPSMPLKLKVILVGERESLADFQE
                     MEPELSEQAIYSEFEDTLQIVDAESVSQWCRWVTFTARHNHLPAPGADAWPVLIREAA
                     RYTGEQETLPLSPQWILRQCQEVASLCDGDTFSGEQLNLMLQQREWREGFLAERMQDE
                     ILQEQILIETEGERIGQINALSVIEFPGHPRAFGEPSRISCVVHIGDGEFTDIERKAE
                     LGGNIHAKGMMIMQAFLMSELQLEQQIPFSASLTFEQSYSEVDGDSASMAELCALISA
                     LADVPVNQSIAITGSVDQFGRAQPVGGLNEKIEGFFAICQQRELTGKQGVIIPTANVR
                     HLSLHSELVKAVEEGKFTIWAVDDVTDALPLLLNLVWDGEGQTTLMQTIQERIAQASQ
                     QEGRHRFPWPLRWLNWFIPN"
     gene            24198..24650
                     /gene="matP"
                     /locus_tag="J1Y83_002096"
     CDS             24198..24650
                     /gene="matP"
                     /locus_tag="J1Y83_002096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrodomain Ter protein MatP"
                     /protein_id="HBA7630243.1"
                     /translation="MKYQQLENLESGWKWKYLVKKHREGELITRYIEASAAQEAVDEL
                     LSLENEPVLVNGWIDKHMNPELVNRMKQTIRARRKRHFNAEHQHTRKKSIDLEFIVWQ
                     RLAGLAQRRGKTLSETIVQLIEDAENKEKYANKMSSLKQDLQALLGKE"
     gene            complement(24728..25768)
                     /gene="ompA"
                     /locus_tag="J1Y83_002097"
     CDS             complement(24728..25768)
                     /gene="ompA"
                     /locus_tag="J1Y83_002097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpA"
                     /protein_id="HBA7630244.1"
                     /translation="MKKTAIAIAVALAGFATVAQAAPKDNTWYTGAKLGWSQYHDTGF
                     IDNNGPTHENQLGAGAFGGYQVNPYVGFEMGYDWLGRMPYKGSVENGAYKAQGVQLTA
                     KLGYPITDDLDVYTRLGGMVWRADTKSNVYGKNHDTGVSPVFAGGVEYAITPEIATRL
                     EYQWTNNIGDAHTIGTRPDNGMLSLGVSYRFGQGEVAPVVAPAPAPAPEVQTKHFTLK
                     SDVLFTFNKATLKPEGQAALDQLYSQLSNLDPKDGSVVVLGYTDRIGSDAYNQALSER
                     RAQSVVDYLISKGIPADKISARGMGESNPVTGNTCDNVKQRAALIDCLAPDRRVEIEV
                     KGIKDVVTQPQA"
     gene            complement(26124..26633)
                     /gene="sulA"
                     /locus_tag="J1Y83_002098"
     CDS             complement(26124..26633)
                     /gene="sulA"
                     /locus_tag="J1Y83_002098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division inhibitor SulA"
                     /protein_id="HBA7630245.1"
                     /translation="MYTSGYAHRSSSFSSAASKIARVSTENTTAGLISEVVYREDQPM
                     MTQLLLLPLLQQLGQQSRWQLWLTPQQKLSREWVQASGLPLTKVMQISQLSPCHTVES
                     MVRALRTGNYSVVIGWLADDLTEEEHAELVDAANEGNAMGFIMRPVSASSHATRQLSG
                     LKIHSNLYH"
BASE COUNT         6725 a         6715 c         6980 g         6351 t
ORIGIN      
        1 tcttatattt aaattaacta aacggaaatt ttgtttctga tggaaacttt atcgacctgg
       61 cacaaaatcc tctttcagac atccagaatg ccaaaaaata gtatgaaaat tgtgctatca
      121 gctaagaaaa aactaatccg caataagaat gcgggttatt ttttgtgcgt aaatgttgca
      181 acgtaatatt ccgtaacagg atgatcgtta tggcggggtt gcaaatagat tgcttgactt
      241 aacaaacaga taacttgaca gaaaggataa aaaaagagcc agcgggtgct ggctcttgaa
      301 gacgctaaat tagaagctgg cgttacgcgg agtacgtggg aacggaatca catcacgcac
      361 gttttgcaca ccagttacgt aagcaatcag gcgttcaaaa ccaagaccga aacctgaatg
      421 cggaacagta ccgtagcgac gcagatcgcg ataccaccag taatcttctt tattcaggcc
      481 catttccagc atacgctcgt ccagcacgtc cagacgttct tcacgctggg agccaccaat
      541 gatctcaccg atgcccggag ccagaacgtc catagccgca acggttttac cgtcttcgtt
      601 aaggcgcata tagaacgctt taatatcttt cggatagttt ttaaccacta ccggtgcttt
      661 aaagtgttct tccgccagat aacgctcatg ctcagaagag agatcgacgc cccagtaaac
      721 cgggttttca aacttcctgc cgcagttttc gagaatggtc actgcgtcgg tgtaatccac
      781 ctgcgcgaaa tcggcctcga tgaagcgttc cagacgtgaa acggcatctt tatctacgcg
      841 ttcagcgaag aatttcatgt cgtcagcgcg ttcttcgaga accgctttga agacatattt
      901 cagcatggct tcagccagac ccgcgatatc gttcaggtta gcaaacgcca cttccggctc
      961 cagcatccag aattccgcca ggtgacggct ggtgttggag ttttcagcac ggaaagtcgg
     1021 gccgaaggta taaattttgg acaatgcgca agcgtaagtt tcgccgttca actgaccaga
     1081 tacggtcagg aaagactctt taccaaagaa gtctttgtcg aaatccactt tgccctgatc
     1141 gttacgcggc aggttttcca gatccagcgt agaaacgcgg aacatttcgc cagcaccttc
     1201 ggtatcagat gcggtaatca gtggcgttga aacccagaag aatccctgct cgttaaagaa
     1261 gcgatgcagc gcctgcgcca gcgtatgacg aacgcgcgcg acggcaccaa tcaggtttgt
     1321 gcgcggacgc aggtgagcga cttcacgcag atactcaatg ctgtggcgtt ttgccgccat
     1381 cgggtaagtg tctggatctt caacccaacc agcaacttca accttgctgg cctgaatttc
     1441 aaattgttgc ccctggcccg gcgacgccac gactttaccc gtcacaatga ccgagcaacc
     1501 ggtggtcaga cgcaggacgt cttcattgta attgggcaga gaattattga tgacagcctg
     1561 tacaggatca aagcaggaac cgtcataaac ggcgaggaag gagatgccag cttttgaatc
     1621 tcggcgggta cgtacccatc cgcgcacggt gacttcgctg tcaacggcta cgcggccctg
     1681 gagtacgtcg gctacaggca caacgctcat aatattctct ctgttaatag tcggaaaaaa
     1741 taaacacttg tccacccgta aatgggggga tacctatgtt acctggcatc tgcaatcaga
     1801 caagcagaat tcgcaaatgc agcgaaagat ttcggaatta aaggtaaaaa aagggagccg
     1861 attagctccc tgatgatatt aactggcttt tttaatatgc ggaaggtcga acgctttgcg
     1921 cagcgcacga acaaacgctt tatcgtggca gatagttttg ccagggctgt cagaaagttt
     1981 cgccaccggt ttaccgttac actcaaccaa cttaatgaca atattcaggg gttttacctg
     2041 ggggatatcg caggtcaggc gagtcccaat accaaaactt aattgcacgc gggaagagaa
     2101 gtggcggtat agctcaaccg ctttgcgtaa atccagattg tcagagaaaa ccagcgtttt
     2161 actctgtgga tcaattccca gcttctcata atgtgcaatg gctttttcac cccattcaac
     2221 tgggtcgccg gagtcatgac gcaagccctg atagcggctg gcgaactcga caccgaaatc
     2281 acgcaggaaa gcatccatag tgatgcagtc ggttaatgca atgccaagtt ggtcgggata
     2341 ttcttccagc caggcagcaa gtgcagctcg ctggctgttg gcaagatccg gactgatttg
     2401 ctgatgcgcc tggaaccatt cgtgtgcttg tgttcccatt ggcgtgaggg aaagccgacg
     2461 cgccagatcg tagttgctgg tgcccacgaa ccaggattcc tgttgcagac gtttcacgat
     2521 ggtttcttgt acttcacgag aaaaacgtcg gcgggtgcca aaatccatca gatggaagcg
     2581 cgacatatca agaccggcgg ttaatgccga gaagtcgact aatttgtttt ccagcgtgtc
     2641 gagggcttgc gcaacgtcgg cctgcggtga gcgatagcga tgtaccattt cactgataac
     2701 cgccagcaaa ggaacttccc agaggatgac ttcacgccac gggccgctta aacgaatatc
     2761 cagcttgcca ttatcgttgg acacggtgac ttgttccggg ttaaagcgga actcgcgtag
     2821 ccagttaaga tagtcggcct taaagaaagg cagggcagag agccactgat attcatcatc
     2881 ctgcaggcgc aggtgctgca tcgcctgaac ctgttcacga atagcatcgg cataaatacc
     2941 cagcagatcg tcacctcggc aacgaaactc cgccgcgaca tgcacatcgt aatagtgatg
     3001 aaacacggct tgctgcatat gcaacttata agcatctgta tccagcaacg agtgcagaac
     3061 aggagaagcg aattgtgtca taggtgcgca gtagcgtcct cgtacaggag cgtttagtac
     3121 aataaacatc ttcaggaact gctggagtat accttgtttc gcaatttatt gaaccccgat
     3181 cacaccatat gccacctttc tggtcgatgg cattacgcgg tgtatgttat aaaaatgtag
     3241 caataaaggc gtttgtacct gaaaagatga agattctgca tagcgcgatt tacgcaacag
     3301 gaatagactg aacaccagac tctataaaag atgctaaagg ttatttatga ctcaacagcc
     3361 acaagccaaa taccgtcacg attatcgtgc gccggattac cagattactg atattgactt
     3421 gacctttgac ctggacgcgc aaaagacggt cgttaccgcg gtcagccagg ctgtccgtca
     3481 tggtgcatca gatgttccgc ttcgtctcaa cggcgaagac ctcaaactgg tttctgttta
     3541 tattaatgat gagccgtgga ccgcctggaa agaagaagag ggcgcactgg tcatcagtaa
     3601 tttgccggag cgttttacgc ttaagatcgt taatgaaatt agcccggcgg cgaatactgc
     3661 gctggaaggg ctttatcagt caggcgatgc gctttgcacc cagtgtgaag ccgaaggttt
     3721 ccgccatatt acgtattatc tcgaccgccc ggatgtgctg gcgcgtttta ccaccaaaat
     3781 tattgccgat aaaaccaaat atcccttcct gctttccaac ggtaaccgcg ttgcgcaagg
     3841 cgaactggaa aacggacgtc actgggtaca gtggcaggac ccgttcccga aaccgtgcta
     3901 cctgtttgcg ctggtggcag gcgactttga tgtgctgcgc gacactttca ccacgcgttc
     3961 tggtcgcgaa gtagcgctgg agctgtatgt cgatcgtggc aaccttgatc gtgcgccgtg
     4021 ggcgatgacc tcgctgaaaa actccatgaa atgggatgaa gaacgcttcg gcctggagta
     4081 tgacctcgac atctatatga tcgtcgcggt ggatttcttc aatatgggcg caatggagaa
     4141 taaggggctg aatatcttta actccaaata tgtgctggcc cgcaccgaca ccgccaccga
     4201 caaagattac ctcgatattg aacgcgttat cggccatgaa tatttccata actggaccgg
     4261 caaccgtgtc acctgtcgcg actggttcca gctcagtctg aaagaaggtt taaccgtctt
     4321 ccgcgatcag gaattcagct ctgaccttgg ttcccgcgcg gtaaaccgta tcaataatgt
     4381 gcgcaccatg cgcggattgc agtttgcaga agacgccagc ccgatggcgc acccaattcg
     4441 cccggatatg gtcattgaaa tgaacaactt ctacaccctg accgtttacg agaagggcgc
     4501 ggaagtgatt cgtatgatcc acactctgct tggcgaagaa aacttccaga aagggatgca
     4561 gctctatttc gagcgtcatg atggtagcgc agcgacctgt gacgactttg tgcaggcgat
     4621 ggaagatgcg tcgaatgtcg atctttccca tttccgccgt tggtacagcc agtccggtac
     4681 gccggttgtg accgtcaaag acgactacaa tccagaaact gagcagtaca ccctgaccat
     4741 cagccagcgc acgccagcta cgccggatca ggcagaaaaa cagccgctgc atattccgtt
     4801 tgccatcgaa ctgtatgata acgaaggcaa agtgatcccg ttgcagaaag gcggtcatcc
     4861 ggtgaattcg gtgctgaacg tcacccaggc tgaacagacc tttgtttttg ataatgtcta
     4921 cttccagccg gtgcctgcgc tgctgtgcga attctctgcg ccagtgaaac tggaatataa
     4981 gtggagcgat cagcaactga ccttcctgat gcgtcatgcg cgtaatgatt tctcccgttg
     5041 ggatgcggcg caaagcctgc tggcaaccta catcaagctg aacgtcgcgc gtcatcagca
     5101 aggtcagccg ctgtctctgc cggtgcatgt ggctgatgct ttccgcgcgg tactgcttga
     5161 tgagaagatt gatccggcac tggcggcaga aatcctgacg ctgccttccg tcaatgaaat
     5221 ggcagaactg ttcgatatca tcgacccgat tgctattgct gaagtacgcg aagcactcac
     5281 tcgtactctg gcgactgaac tggcggatga gctactggct atttacaacg cgaattacca
     5341 gagcgagtac cgtgttgagc atgaagatat tgcaaaacgc actctgcgta atgcctgcct
     5401 gcgcttcctt gcttttggtg aaacgcatct ggctgacgtg ctggtgagca agcaatacca
     5461 tgaagcaaac aatatgaccg atgcgctggc ggcgctttct gcggcggttg ctgcacaact
     5521 gccatgccgt gacgcgctga tgcaggagta cgacgacaag tggcatcagg atggtctggt
     5581 gatggataaa tggtttatcc tgcaagccac cagcccggcg gcgaatgtgc tggagacggt
     5641 acgtggtctg ttgcagcatc gctcatttac catgagcaac ccgaaccgca ttcgttcatt
     5701 gattggcgca tttgcgggca gcaacccggc agcgttccat gccgaagatg gcagcggtta
     5761 tcagttcctg gtggaaatgc ttaccgacct caacagccgt aacccgcagg tggcttcacg
     5821 cctgattgag ccgctgattc gcctgaaacg ttacgatgcc aaacgtcagg agaaaatgcg
     5881 cgcggcgctg gaacagttga aagggctgga aaatctctct ggcgatctgt acgagaagat
     5941 caccaaagca ctggcttgat acttttgagc caaaatgctt gacgcgtctt atcaggccta
     6001 caagtccgtt gaaattgctg gttttgtagg ctggataagg cgttcacgcc gcatcaggca
     6061 gtcgtgccca actgcctgat gcgacgcttg acgcgtctta tcaggcctac aatcccgttg
     6121 aaaatgctgg ttttgtgcgc cacatccggc atattaacca tgacgtcgaa tcggttgttc
     6181 agagtgccca cgttgcatca cccgttgcaa cacttccgct tccagctccg ctaatcgcac
     6241 cgaccctaac cggcgcggac gggggatatc caccgtcaaa tccagaccaa tttttccctc
     6301 ttcaattaac aacacccggt cagccatcgc caccgcttcg ctcacatcat gcgtcaccag
     6361 caaaacggta aagccgtgct cctgccagag tgacacaatc aaatcctgca tctcgagtcg
     6421 cgttaaagcg tccagcgcac cgagtggttc atcaagcaac aataaacccg gtcgatgaat
     6481 cagcgccctt gccagcgcca ctcgctgttt ctgcccgcct gaaagtgcgg caggccattc
     6541 ccctgcgcga ttctccagtc ctactgcagc caacgcccga cgcgcggcat cgcgccactg
     6601 gcctttaagg cctaacccaa cgttatcaat caccgatttc cacggcagca gacgcgcatc
     6661 ctgaaacatc attcgcgtgt cttcctgaat ttcagccagc ggtgtggtgc ccgctaacac
     6721 atcgccagcg gttggcgttt ccagacctgc cagcaggcgc agcagggtac ttttgccacc
     6781 gccgctgcgg cccaccaccg ccacaaactg acctgccgga atatgtaaat ccagttggtt
     6841 caggacgata ttttccgcgt aatgtttgct tactgcattg agcaacaatg gtgtgccctg
     6901 gttcagacga gcagtattca taccgtggcc tccttcaaat gataagccgg gttccagcgc
     6961 aaccagagac gctctaacag ctgcgcgctg acgtcagcaa gtttgccgag cagggcgtaa
     7021 agaataatgg cgactaccac cacgtccgtt tgcagaaact cccgcgcatt catcgccaga
     7081 taaccaatgc ctgaattggc agaaatagtt tcggcaacga tcagcgtcag ccacatcagg
     7141 cccaaagcaa aacgcacacc gaccataatt gagggcaggg caccaggcag gatcacatgg
     7201 ataaacagcg gtatgccgga taatccatag ctacgtgcca tctccaccag cccacggtcg
     7261 atattacgga tgccgtgcca ggtgttgata taaatgggga atagcgtacc gagcgccacc
     7321 agaaagattt ttgcggattc atcaatgcca aaccacaaaa tcaccagcgg gatcagcgcc
     7381 agatgcggca cgttgcgcaa catctgaatg gaggtatcca gcagccgttc tccccagcgc
     7441 gacagcccgc taatcagccc cagaattaat cccagcgatc cgccaattga aaaaccaatc
     7501 agcgcacgcc aggagctgat cgccagatgc tgccacagtt cgccgctggc agagagcgtc
     7561 cagaacgcca tcaccactcc ttccggtgag ggcaaaatac gcgtcgatag ccagccaacc
     7621 gaggaggcca gttgccacac cgccacgatg cccaccggta aaaaccaggg agcaacgcgc
     7681 aataaccact tcttcactgg cgttgccatc atcgtgttcc ttagctttgc gcgacgttac
     7741 gggggataaa atcattcgcc accgcttcgc cttgtggatt cagcggttgt ggctggggaa
     7801 tttccgggat ggcgacatcc agatgtggga acagtaactc gccgacccga tacgcttctt
     7861 ccagatgcgg gtagcccgaa agcacaaaac tgtcgatgcc aagtgcagcg tattcgttga
     7921 ttcgcgcggc gaccgtagga ccatcgccca ccagcgccgt cccggcaccg ccgcgcacta
     7981 agccaacacc cgcccataaa ttggggctga tctccagatt gtcgcgcttg ccgttatgta
     8041 gcgccgccat tcgctgttgt cctacggaat ccgttcgggc gaatgcggcc tgtgctttgg
     8101 cgatagtttc atcatcaaga tgcgagatta accgctcggc ggcctgccac gcttcgtcgt
     8161 tggtttcacg aacaatcaca tgcagacgaa taccgaaacg aattttgcgt ccatgcgcgg
     8221 cagctttcgc ccgcacgtgt tcgattttct cttttaccag ttccggtggt tcgccccagg
     8281 taaggtagag atcaacttgt tctgccgcca gctcctgggc gacatctgac gatccgccaa
     8341 agtaaagcgg aggataaggc tgttgaatcg gcgggaagag cagtttggct ccgcgtacat
     8401 gaatatgttt gccgttgaaa tccactgtct cgccaagcaa caaccgccgc cagacctggg
     8461 taaattctgc cgaggcttcg taacgctcgc tatgatcaag gaacacgccg tcgcctgcca
     8521 gttcttgggg atcgctgcct gtgaccaggt taaacaacgc acgtccgtta gagagacggt
     8581 caagcgtggc ggcctggcgg gcggcaacgg taggtgaggt tacgctgggg cgcagggcga
     8641 caagaaactt cagccgttgt gtcaccggga tcatcgacgc cgccaccagc cacgcatctt
     8701 cacacgatcg ccccgtcggg atcagcacac cggtatagcc cagacgatcc gccgcttgcg
     8761 caatttgttg cagataaccg tgatcaaccg gacgtgaacc ttcttccgtt cccagataat
     8821 gcccgtcacc gtgggtcggt aaaaaccaga acatattcag actcataatt gttttccttc
     8881 cagttgagtg ggctgccaga tgcgctggcg aatatcgact tttttcggca ccagacggtt
     8941 ttcataaaac agatctgccg tgtgctgctg taaggcggca acttcagcgc tcaacggttt
     9001 gatggtggtg ggaggacgat gatccagata cgaggcaata accggtgccg gtaagcccat
     9061 cgtttttgcc agtaaagcga tgctttgttc gcgctggctg cgggttaacg catcggcctc
     9121 actaaaggtt gtcagcacac cctgaataaa agcgccgttt ttttctgcat acggacgagc
     9181 tgccagataa aacgatccag tttgattgag atcggtgccg tctttcagca cccgcacacc
     9241 gccctgtaat aatgcagcgg agtagtaggg atcccagata gcccaggcgt caacgttacc
     9301 ttgctggaac gcggcgcggg catcggcagg cgtcaggtaa gtgggctgaa tatccgtgaa
     9361 tttaagcccg gcctggcgaa gggctcgcag taaaagattg tgcgaactgg aacctttctg
     9421 aaaggcaact ttgtgacctt taagatcggc tacggttttg atcgggctgt tttctgccac
     9481 cagaatcact tcggctttgg gcttcggtgg ctcgacgccc acgtatacca aatcagcccc
     9541 ggcagcctgg gcaaagattg gcggaatatc tccggtactg ccgagatcga tactgccaac
     9601 gtttaacgcc tccagcatct gtgggccagc ggggaactcc acccaggaga tttttgtttg
     9661 cggatagcgt ttttccagta actggtggct ttttgccagc accataccaa tactgccttt
     9721 ttgatagcct atacgtaacg cctctggcga agactccgcg gcaacagcaa gtgtagagac
     9781 gctaagtaaa cccgccagcg ccagtttaat gatcttacgc atgggcatta cctcgcagag
     9841 acagaaggtc aggaacctga acatcgcggc ggtgcaacgc ctgccagaaa gtttctagcg
     9901 cggtatcaag acgagtttgc agatttggcg tgaacttggg tttgtgttgg taatcaatga
     9961 cttgcgagtc atcggcaaac acgccgtgca ggatctcctg ggctttcagc gtacttaaaa
    10021 ctggcttaag ggcataatcg accgccagca gatgggccac ggtgccgcct gtcgccagcg
    10081 gcagcaccac tttgccttgc aacgcacgtt ctggcagcag gtcgagcagg gttttcagtg
    10141 caccggaata ggcggcttta tacacaggcg tggcgacaat cagcccatcg gcctgttgca
    10201 gctgttcggt gaaggtcttg agtgccggac tatcgaaacg agcataaagc agatcttccg
    10261 ggacgaagtt ttgcaaattc cagtgataaa cctctacatc caggccattt agtttttccc
    10321 gcacatattc cagcaaggag ctggagcgag aaggaaagcg aggactaccc gccagggtga
    10381 tgacacgcat actctctcct tataaccaat tgttcttgtt ttgttaacat tgataacaat
    10441 tcggtcagtc tgacagaggg cgtccgcgag tcgaaatgat ttatctgagg aagaaaagcc
    10501 aaaaaaagca taaaaaaagc gcaataagaa aacgtttgct atttattgcc gcaggtcaat
    10561 tcccttttgg tccgaactcg cacataatac gcccccggtt tgcacaccgg gaatccagga
    10621 gagttcatgt actacccctt cgttcgtaaa gcccttttcc agctcgatcc agagcgcgct
    10681 catgagttta cttttcagca attacgccgt attacaggaa cgccgtttga agcactggtg
    10741 cggcagaaag tgcctgcgaa acctgttaac tgcatgggcc tgacgtttaa aaatccgctt
    10801 ggtctggcag ccggtcttga taaagacggg gagtgcattg acgcgttagg cgcaatggga
    10861 tttggatcga tcgagatcgg taccgtcacg ccacgtcccc agccaggtaa tgacaagccg
    10921 cgtctctttc gtctggtaga tgccgaaggt ttgatcaacc gtatgggctt taataatctt
    10981 ggcgttgata acctcgtaga gaacgtaaaa aaagcccatt acgacggcgt tctgggtatt
    11041 aacatcggca aaaataaaga tacgccggtg gagcagggta aagatgacta tctgatttgt
    11101 atggaaaaaa tctatgccta tgcgggatat atcgccatca atatttcatc gccaaatacc
    11161 ccaggattac gcacactgca atatggtgaa gcgctggatg atctcttaac tgcgattaaa
    11221 aataaacaaa atgatttgca agtgatgcac cataaatatg tgccgatcgc agtgaagatc
    11281 gcgccggatc tttctgaaga agaattgatc caggttgccg atagtttagt tcgccataat
    11341 attgatggcg ttattgcaac caataccaca ctcgatcgtt ctctggttca gggaatgaaa
    11401 aattgcgatc aaaccggtgg cttaagtggt cgtccgcttc agttaaaaag caccgaaatt
    11461 attcgccgct tgtcacagga attaaacgga cgcttgccga ttattggtgt tggcggcatt
    11521 gactcggtta tcgctgcgcg tgaaaagatt gctgcgggtg cctcactggt gcaaatttat
    11581 tctggtttta tttttaaagg tccgccgctg attaaagaaa tcgttaccca tatctaatta
    11641 tttcttcaaa ttcctcttta taagataatc agggctttat tttcagccct ggttgtttta
    11701 tattcatcgc tgttgcttat ttagacattt tgtactttta ttgttgaggt tattaagcga
    11761 agcgacaatg gattgtgtcg ctgcggtttt ataaggttgg ggagtggcag atgcgaatta
    11821 aaccagacga taactggcgt tggtattacg atgaagagca cgatcgaatg atgctcgatt
    11881 tagccaatgg tatgctattt cgctcacgtt ttgcgcgcaa aatgttgact ccagatgcct
    11941 tttctcccgc aggtttttgc gttgatgacg ccgcgctcta tttctctttt gaagaaaagt
    12001 gccgcgattt taatttatct aaagaacaaa aagccgaact ggtgctgaat gcactggtag
    12061 caattcgtta tctcaaaccg caaatgccga aaagctggca ttttgtatct catggtgaaa
    12121 tgtggatgcc catgccgggc gatgcggcct acgtttggtt gagcgacact catgagcaag
    12181 tgaatttact ggtcgttgaa tctggcgaga atgccgcgct atgtctactg gcacaaccct
    12241 gcgttgttat tgcgggtcgc gcgatgcagt tgggcgacgc cattaaaatc atgaacgaca
    12301 ggctgaaacc gcaggttaat gttgacagct tcagcctcga acaggcagtc taacgcgcca
    12361 gcttaagcgc agtcttcggt acacaactac agcaaagaat agtgccatca tcgcctattg
    12421 ctgatttttt tagcggcgtg acttcgcctt ctaaaagctg aacacggcaa cttccacaaa
    12481 tgcccgcgcg gcaagaataa gggatacgaa ttccctgatt ttctaattgc tccagcagca
    12541 cctgttggtt atttccacga aatgcctgtc cctgccagtc aatatctacg tttgcgtccg
    12601 gttgttgcgt gatgttggca gtatcatcag cggcacctgc gccgtaaatt ttagccggag
    12661 ccgttgccag aatttccacc tcgtcgccaa cgcgaatcac gccgctatta cgggcaatta
    12721 aattctgacc aaaatcgaca tcgccgttat cctgggcagt acggaaagat tgcagcgttt
    12781 ttaatggttc gcctgccgga tgtttttgcc ctttttctgg gctgacggtg gtgaaaatac
    12841 agcggctaca aggcttaacc acatcaaaca cgacatcacc aatgcgaatc actttccagc
    12901 tatcttcttc ccaggccgat gcaccggaaa ccaccagatt ggggcggaat tgctccattt
    12961 ttacactggc cggacaacgt tgttggagat cacgtaacga ggcctcgtta gcaagaaggt
    13021 aaggatagcc atcagcaaat gacaggggta cagtgttgtg gcgtttcacg cgccgggtca
    13081 tttgtggccc cacccagcgt aattgcactt cgcgggagaa aaatccactt agccatttgt
    13141 tgatcgcgtc tggcgcaatt cgcgcggtaa aatgtgtgcc ccaaacttcg gttggtgcgt
    13201 cttgtgtggc gaaatcagca aaacgaacat atgcactact gccatctggt gcggtgagat
    13261 gcaagccatc atgcacgggt gaaggagtaa accgtaccat tagcggaaac tggcgagcgg
    13321 taataaacgt accgtcaggt tccgtgatca taaagatgcg atcgaaggcc agaccactga
    13381 catctgccag ggtatgtgta agaccaatgc cgcgcatcga tttaacagga tgaataaaaa
    13441 gccgggttaa tgtcgccacg gtgcggtcct caaatgaaaa taagccctca actttatgac
    13501 atgacgcgct tattagctat aatgcgcaac aattttctta gagtaaaagt gacaatatga
    13561 attctctgtt tgccagtacg gcccgtgggc tggaagagct gttaaaaact gaactggaaa
    13621 acctgggggc cgttgaatgc caggtggttc agggtggggt ccatttcaag ggcgacacac
    13681 ggcttgttta ccagagcctg atgtggagcc gcctggcctc gcgtattatg ttgccgctgg
    13741 gcgagtgtaa ggtttacagc gatttagacc tctatctcgg tgttcaggcg atcaactgga
    13801 cagagatgtt taatcctggc gcgaccttcg ctgtccactt cagtggtttg aatgacacca
    13861 tacgcaacag tcagtacggt gcgatgaaag tgaaagacgc gatcgtcgat gctttcacgc
    13921 ggaaaaatct gccgcgtcca aatgttgatc gcgatgcgcc ggatatccgc gttaacgtct
    13981 ggctgcataa agaaaccgcc agtatcgccc ttgatctcag tggtgatggt ttacatctgc
    14041 gtggctatcg cgatcgtgct ggcattgcgc cgatcaaaga aaccctggca gccgctattg
    14101 tgatgcgatc cggctggcag ccaggaacac cgctgctcga tccaatgtgt ggttcaggta
    14161 cgttgctgat tgaagcggct atgttggcga ccgaccgcgc accgggcttg caccgtgggc
    14221 gttggggctt tagcggctgg acgcagcatg atgaagctat ctggcaggaa gtgaaagcgg
    14281 aagcgcaaac tcgtgcccgt aaaggcctgg cggagtatag ctcccatttc tacggttcgg
    14341 acagtgacgc acgggtgatt caacgtgcgc gcactaacgc ccgtcttgcg gggattggtg
    14401 aactgatcac ctttgaggtg aacgatgtcg cgcaactggc caatccgctg ccgaaagggc
    14461 cgtacggtac agtgttgagc aacccgccat acggtgaacg tctggacagc gaaccggcgc
    14521 tgattgcgct gcatagtctg ctgggccgaa tcatgaaaaa ccagttcggt ggctggaatc
    14581 tctctttgtt tagtgcctcg ccggatttgc taagctgcct gcagctgcgt gcagacaaac
    14641 agtacaaggc gaaaaacggc ccgctggact gcgtacagaa aaactaccat gttgccgaaa
    14701 gcacaccaga cagcaaaccg gtaatggcag cggaagacta cgccaaccgt ctgcgtaaga
    14761 atctcaaaaa attcgagaag tgggctcgcc aggaaggaat tgaatgttac cgcctgtatg
    14821 acgccgatct gccggaatat aacgttgccg ttgaccgtta tgccgactgg gtggtggtgc
    14881 aggagtatgc gccgccaaaa actattgatg cgcacaaagc gcgtcagcgt ctgttcgata
    14941 ttatcgctgc aaccatttcg gtactgggga ttgcgccaaa caaactggtg ctgaaaaccc
    15001 gtgaacgcca gaagggcaaa aatcaatacc agaaactggg cgagaagggc gagtttctgg
    15061 aagttaccga atataacgct cacttgtggg tgaacctgac ggattacctc gataccggtc
    15121 tgttcctcga tcaccgtatc gcccgtcgta tgctcggtca gatgagcaaa ggcaaagatt
    15181 tcctcaacct gttctcttat accggcagcg ccaccgtgca tgcgggatta ggcggtgcac
    15241 gcagcaccac caccgtggat atgtcgcgta cttatctgga gtgggcagaa cgcaacctgc
    15301 gtctgaatgg cctaaccggg cgtgcgcatc gcctgattca ggccgattgc ctggcgtggc
    15361 tgcgtgaggc aaatgaacag tttgatctga tctttatcga tccgccaacc ttctctaact
    15421 caaaacgaat ggaagatgcg tttgatgttc agcgcgatca tctggcgctg atgaaagatt
    15481 tgaaacgtct gctgcgtgca ggtgggacga tcatgttctc gaacaacaaa cgcggcttcc
    15541 gtatggatct cgacggcctg gcgaaactgg gactgaaagc acaagaaatt acgcaaaaaa
    15601 cgctctccca ggatttcgcc cgtaaccgcc agatccacaa ctgctggctg attaccgcag
    15661 cctgaaagga atagtaatgt cattaatcag tatgcatggc gcatggctgt cgttcagcga
    15721 cgcgccgctt ctcgataacg cagaactgca tatcgaagat aacgaacgtg tttgtctggt
    15781 ggggcgtaac ggtgcaggca aatcaacgtt aatgaagatc ctcaaccgtg aacaggggct
    15841 ggatgacggt cgcattattt atgagcaaga tttgattgta gctcgtctgc aacaggatcc
    15901 gccgcgtaac gttgagggta gcgtttatga tttcgttgcc gaaggcattg aagaacaagc
    15961 ggaatacctg aaacgctatc acgatatttc gcgcctggtg atgaacgacc cgagcgagaa
    16021 aaatctcaac gaactggcga aggttcagga acagctggat caccacaacc tgtggcagct
    16081 ggaaaaccgc atcaacgaag tgctggcgca actgggatta gatcctaatg ttgcgctgtc
    16141 gtcgctttcc ggcggctggt tgcgtaaagc ggcattagga cgcgcgctgg tgagcaatcc
    16201 gcgcgtattg ctgctcgacg aaccgactaa ccacctggat attgaaacca tcgactggct
    16261 ggaagggttc ctcaaaacct tcaatgggac aattattttc atttcccacg accgttcgtt
    16321 tatccgcaat atggcgacgc gcattgttga tctcgatcgc ggcaagctgg tgacctatcc
    16381 agggaattac gaccagtacc tgctggaaaa agaagaagcc ctgcgcgtgg aagaattaca
    16441 aaatgccgag ttcgatcgca aactggcgca ggaagaggtg tggatccgcc aggggatcaa
    16501 agcacgtcgt acccgtaatg aaggccgcgt acgcgccctg aaagcaatgc gtcgcgagcg
    16561 tggtgaacgt cgcgaagtga tgggtaccgc aaagatgcag gtggaagaag ccagccgctc
    16621 cggtaagatc gttttcgaaa tggaggacgt ttgctaccag gttgacggta agcaactggt
    16681 gaaagatttt tctgcccagg ttctacgcgg cgacaaaatt gccctgattg gtccgaatgg
    16741 gtgcggcaaa accacgctgc tgaaactaat gctcggtcag cttcaagcag acagcgggcg
    16801 tattcatgtt ggcaccaaac tggaagtggc ttatttcgat cagcaccgcg cggaactgga
    16861 tcccgataaa acggtgatgg ataaccttgc cgaaggtaag caagaggtga tggttaacgg
    16921 caagccacgc cacgtattgg gctatttgca ggacttcctg ttccatccaa aacgggcgat
    16981 gacgccggta cgtgcgcttt ctggcggtga gcgtaaccgc ttgttgttgg cgcgtttgtt
    17041 cctcaaacca agcaacttat tgattcttga cgaaccgacc aacgatcttg atgtcgaaac
    17101 gctggaactg ctggaagaac tgatcgacag ctatcagggc acggtattgc tggttagcca
    17161 cgatcgtcag tttgtcgata acaccgttac agaatgctgg atcttcgaag gcggcggtaa
    17221 aattggtcgt tatgttggcg gttatcatga tgcccgtggt cagcaagaac agtatgtggc
    17281 gctcaaacag cctgcggtga aaaaaagcga agaagccgcc gcgccaaaag cagaaactgt
    17341 aaaacgcagc agtagcaaac taagctataa attgcagcgt gaactggagc tgctaccgca
    17401 attgcttgaa gatctggagg cgaagctgga agctttacag acgcaagtgg cggatgcttc
    17461 cttcttcagt cagccgcatg agcagacgca aaaagtgctc gccgatatgg ctgctgcaga
    17521 gcaggagctg gagcaagcct ttgaacgctg ggagtatctt gaagcgttaa aaaatggtgg
    17581 ctgatcgcaa aatagtcgat ttaaacagag actgctgaca acggtaaaag tgcctgatgt
    17641 actgggttta tcaggcatga aaaccaacac tttgttagtc atctgcaaaa tagcgcatca
    17701 ttaaggagta ccaatgtgcg aacatcatca tgccgcgaag cacatcctgt gctcgcagtg
    17761 tgacatgctg gtggcgttac cgcgccttga gcatggtcag aaagcggcat gtccccggtg
    17821 tggcacaacg ttaaccgtgg cgtgggatgc ccctcggcag cgtcccaccg cctatgcgtt
    17881 ggctgcactg ttcatgctgt tgctgtccaa cttgtttcct tttgtgaata tgaacgttgc
    17941 aggagttacc agtgaaatta cattactgga aattcccggc gtgctttttt ctgaggacta
    18001 cgccagcctc ggcacctttt tcctgttgtt tgtgcaactg gttcccgcgt tttgtctgat
    18061 aaccattctg ttactggtga atcgcgcgga attaccggtc cgtttaaaag agcaactggc
    18121 acgggtgctt tttcaactca aaacctgggg aatggcggag attttcctcg cgggtgtgct
    18181 ggtcagtttc gttaaactga tggcttacgg cagcattggg gtaggcagca gctttctccc
    18241 ctggtgttta ttttgtgtcc tgcaactgcg cgcttttcag tgcgttgatc gtcgctggtt
    18301 atgggacgac atcgccccga tgccagaact gcgccagccg ctaaaaccag gtgtcacggg
    18361 gatacgtcag gggctgcgtt cgtgctcctg ttgtacggca atccttcctg ctgatgaacc
    18421 cgtgtgcccg cgttgtagta ccaaagggta cgttcggcgt agaaacagcc tgcagtggac
    18481 actcgcgctg cttgtaacgt ccatcatgct gtatcttccg gctaatattt tgcccatcat
    18541 ggtgacggat ttattaggct cgaagatgcc gtcgacgatt ctcgctgggg tcattctgtt
    18601 atggagcgaa ggctcttatc ccgtcgctgc ggtgattttt ctggccagta ttatggtgcc
    18661 aacgttaaag atgatcgcca ttgcgtggct gtgttgggat gtcaaaggac acggcaagcg
    18721 cgacagtgaa agaatgcatt tgatttatga agttgttgag tttgtaggcc gctggtcgat
    18781 gattgacgtt ttcgttatcg cggtgctctc ggcgctggtg cgtatgggag gtttaatgag
    18841 tatttatccg gcaatgggtg cattaatgtt tgctttagtc gtcataatga caatgttttc
    18901 tgctatgacg tttgacccgc gtttgtcgtg ggatcgtcaa cctgaatcag agcatgagga
    18961 gtcctgacag tatggaatct aataatgggg aagccaaaat ccagaaagtg aagaactggt
    19021 ctcccgtgtg gatatttcct atcgtcacgg cgctcattgg ggcctgggtt cttttttatc
    19081 attacagcca tcagggaccg gaagtgaccc tgatcaccgc gaatgcggaa ggaattgaag
    19141 gtggcaaaac caccattaaa agccgtagcg ttgacgtcgg cgtggttgaa agcgccacac
    19201 tggctgatga tttgacacac gttgaaatca aagcgcggct gaattccggt atggaaaaat
    19261 tgctgcataa agataccgtc ttttgggtgg tgaaaccgca gattggtcga gaagggatta
    19321 gcggcctggg aacgctgctg tctggggttt atatcgaact gcagccaggc gcgaaaggca
    19381 gcaaaatgga taaatacgat ttgctggact cgccaccgtt ggccccgcct gatgcgaaag
    19441 gtatccgtgt gcttctcgat agcaaaaaag ccgggcagct ctcgccagga gatccggtgc
    19501 tgttccgtgg ctatcgggta ggttcggttg aaaccagcac tttcgatacg caaaaacgca
    19561 atatcagcta tcaactgttc atcaatgcac cttatgaccg actggtgacc agcaatgttc
    19621 gtttctggaa agatagtggc attgcggttg atctgacgtc agcgggaatg cgtgtggaga
    19681 tgggctcatt gacaacgctg cttagtggcg gtgtcagctt tgatgtgccg gaaggtctgg
    19741 atttagggca gccagtggca ccgaaaacag ctttcgtttt gtatgatgat cagaagagca
    19801 ttcaggattc gttgtacacc gatcacattg attatctgat gttctttaaa gattcggtac
    19861 gcggtctgca accgggagct ccggtagaat tccggggtat tcgcctgggt accgtaagca
    19921 aagtgccatt ctttgcgccg aatatgcgtc agacatttaa cgatgattac cgtattccgg
    19981 tactgattcg tatcgagcca gagcggctga aaatgcagct tggcgaaaat gcggatgttg
    20041 ttgagcacct tggcgaattg ttgaaacgtg gtttacgcgg atcgctgaaa accggaaacc
    20101 tggtcactgg cgcactgtat gttgatctcg atttctatcc aaatacgcct gcaataaccg
    20161 gtattcgtga atttaatggt tatcagatta tcccgaccgt cagcggcggc ctggcgcaaa
    20221 tccagcaacg actgatggaa gcgttggata agatcaacaa actgccactg aatccgatga
    20281 ttgaacaggc aaccagtacg ctttctgaaa gtcagcgcac aatgaaaaac ctgcaaacga
    20341 cgctggatag catgaacaag atccttgcca gccagtcgat gcagcagtta ccgacggata
    20401 tgcagtcaac gttgcgtgaa ttgaatcgca gcatgcaggg cttccagcct ggttccgcag
    20461 cctacaacaa gatggtggcg gatatgcagc gccttgatca ggtgttgcga gaactgcaac
    20521 cggtgctgaa aacgctcaac gagaagagta acgcgctggt atttgaagcg aaggacaaaa
    20581 aagatccaga gccgaagagg gcgaaacaat gaaaaagtgg ctagtgacga ttgcggcact
    20641 gtggctggcc ggatgcagct ccggcgaaat taataaaaac tattaccagt tacctgtggt
    20701 gcagagcggt acacaaagca ccgccagcca gggtaatcgc ctgttatggg ttgagcaggt
    20761 cgctgttcct gactatctgg cggggaatgg tgtggtttat caaaccagtg atgtgaagta
    20821 tgtgattgcc aacaacaacc tgtgggccag cccgttggat caacagttgc gcaacaccct
    20881 ggttgccaac ctgagtacgc aactgcccgg ctgggtggtt gcctcccagc ctctgggaag
    20941 cgcccaggac acgctcaatg ttaccgtcac ggagtttaac ggtcgctatg atggcaaggt
    21001 cattgtcagt ggtgagtggc tgttgaacca ccagggacaa ctgatcaaac gtccgttccg
    21061 tctggaaggg gtgcaaactc aggatggtta cgatgaaatg gttaaagtgc tggccagtgt
    21121 ctggagtcag gaagccgctt ctattgcgca agagataaag cgtctaccgt aatcataaag
    21181 atttgtaaat ataaccgtct ccggtatgtt gcctgaggcg gtttttttgt ttctaacgtg
    21241 cggaaaaagt tgttcctctt cacatttttt gtacaaccga catgcccgtg tagctcacaa
    21301 atatgacagt ggcgtgaatt ttgcacattg acggcagtta tgattcgcgg tattgcttaa
    21361 ctgtgattgc atatttagta atcactgttt tcttttccac cagaaaccag tatgagggaa
    21421 acgaggcatg aagagacaaa aacgagatcg cctggaacgg gcacatcaac gtggttatca
    21481 ggccggcatc gccggacgct caaaagaaat gtgtccctat cagacgctga atcaaaggtc
    21541 acaatggctg ggaggctggc gagaagccat ggcggacagg gtagtcatgg cctgattctg
    21601 tctctttaaa aagaaacctc cgcattgcgg aggtttcgcc tttatatact ctgtctgatt
    21661 aaaatcagaa tgcagacgta tcctggaaca gacctacttt caggtcgttg gcggtataga
    21721 tcaaacggcc atcaaccagc acttcgccat cagccaggcc cataatcaga cgacggttaa
    21781 ctatgcgttt aaagtgaata cgataggtca cttttttcgc cgtcggcagc acctggcctg
    21841 tgaatttcac ttcacctacg cccagcgcgc gacctttacc ttcgccgccc agccagccga
    21901 ggtagaaccc taccagctgc cacattgcgt ccaggcccag gcatcccggc ataaccggat
    21961 cgccgataaa gtggcatccg aagaaccaca gatccggatt gatatccagc tctgcttcaa
    22021 catacccttt gtcgaagtta ccacccgttt cggtcatttt gaccacacgg tccatcatca
    22081 gcatgtttgg tgctggcaat tgcgggcctt tagcaccaaa cagttcaccg cgaccagagg
    22141 caagaaggtc ttcttttgta taggattcgc gtttatctac catgttctct gtaagcctta
    22201 ttttattgaa gcacgcagga tagctaacac gtgtacgctg aacaagtccg atcagttcgg
    22261 aataaaccag ttcagccaac gtaatggcca tggaaaacgg tgacgtcctt cctgttgcga
    22321 tgcttgtgcg atacgttcct ggatggtttg catcagcgtc gtttggcctt cgccatccca
    22381 caccagattt aataataacg gcagtgcgtc agtcacatcg tctactgccc agatggtgaa
    22441 tttgccttct tctaccgctt tcaccagttc actgtgaaga cttaaatggc gaacgttagc
    22501 agtggggata atgacacctt gtttccccgt taactcacgt tgctggcaaa tagcaaagaa
    22561 gccttcgatt ttctcattta aaccaccgac tggctgggcg cgaccgaact gatcgactga
    22621 acctgtgata gcgatactct gattcaccgg cacatcggcg agggcgctta tcagggcgca
    22681 gagttcagcc atcgaggcac tatcgccatc cacttcactg tatgactgct caaatgtcag
    22741 cgatgctgag aaggggatct gttgctcaag ctgtagttcc gacatcagga acgcttgcat
    22801 gatcatcatc cctttcgcat ggatattgcc gccaagctcc gctttgcgtt cgatgtcggt
    22861 gaattcacca tcgccaatat gcacaacgca gctaatgcga gaaggttcgc caaaagcgcg
    22921 tggatgaccc ggaaattcaa tgaccgaaag ggcgttaatt tgcccgatgc gttcgccttc
    22981 ggtttcaatc aggatttgct cctgaaggat ctcatcctgc atgcgttcag cgaggaaacc
    23041 ttcgcgccat tcacgctgct gtagcattaa gtttagctgc tcgccggaga aggtgtcgcc
    23101 atcacacagg gaggcgacct cttgacactg gcggaggatc cactgcgggc taagcggaag
    23161 tgtttcttgt tcaccagtgt agcgtgctgc ttcgcggata agtaccggcc aggcatccgc
    23221 tcccggtgct ggtaagtgat tatgtctggc ggtaaatgtc acccagcgac accactggct
    23281 tactgactcc gcatcgacaa tctgcagagt atcttcaaat tcgctataaa tagcctgctc
    23341 tgaaagctct ggctccatct cctggaaatc agccaatgat tcgcgttcgc ctaccagaat
    23401 gactttcagc ttcaatggca tcgaaggcac agagacgggg agagggcgcg actcatcaaa
    23461 cgcaacccag tcaaaacgct cgcggttaac gatatttttc agccgcatcc acagcagagg
    23521 ttgcgccagc agtgtacgca aagagatgat gagaataccg ccatttgcct gatgcaccag
    23581 accaggctgc agggtaatat cgccattaaa ctgacgcagg cagccaaaga gttgctctgc
    23641 ttctacccag tcggcagcga caacttgcgt taaagtcgca aaattatcat ctgcactcac
    23701 tgcgtgacgt aagcggatgg tgtggccgga aacctcatag tgaccgccca ccagttggcc
    23761 tgcatcgctt tgtagcgtac gcgcggcatc agcaataaga ttcagatact cagactcttc
    23821 cggggccttc gccagcataa aagaggagga tgctcgcgta tgcagcaact gctccagcgc
    23881 aaattgcagt cgcggttgag tatcactgaa taaaggatcg ttttcgtcaa tcaaatgtgg
    23941 ctgagcaaat atttcctgat agctatcggt atcaggaacc aggtcacgcc atgcaagttt
    24001 cgtaatggtc aaagttgatg ttttttagtc tgttgtcaaa gccgcgatta taccgtaacc
    24061 ggcactgcag cacacgtaga aagcaacgac aatactcctg gcatgggcgt taaagctcac
    24121 aggatggaga ttcttttctt cactggccta aaaagctgat attctgtaaa gagttacacg
    24181 gtaacattga gatcgctatg aaatatcaac aacttgaaaa tcttgaaagc ggttggaaat
    24241 ggaagtatct ggtgaagaag caccgcgaag gggagttaat cacccgttac atagaagcca
    24301 gtgccgccca ggaagccgtc gatgagttgc tctcgctgga aaatgaaccc gtgctggtaa
    24361 atggttggat tgacaagcat atgaatccgg aactggtcaa tcgcatgaag cagaccattc
    24421 gggccagacg gaagcgccat tttaatgcag aacaccagca tacgcgcaaa aaatcgatcg
    24481 atctggaatt tatcgtctgg caacgtctgg ctggccttgc gcagcgtcgc ggtaaaacgc
    24541 tttctgaaac gattgttcag ctgattgaag atgcggaaaa caaagagaaa tacgcgaata
    24601 aaatgtcttc tttgaagcag gatctgcagg cattgctggg taaggaataa ctgtcaaaag
    24661 tcagttcaat ttactaaagg cgaaaaaaaa accccgcagc tgcggggttt ttctaccaga
    24721 cgagaactta agcctgcggc tgagttacaa cgtctttgat gcctttaact tcgatctcta
    24781 cgcgacgatc cggagccaag caatcgatca gtgcagcacg ctgtttcacg ttgtcacagg
    24841 tgttgccagt aaccgggttg gattcgccca taccacgtgc ggagattttg tctgccggga
    24901 tacctttaga gatcaggtaa tcaacaacgg actgagcacg acgctcggac agagcctggt
    24961 tataagcgtc agaaccgatg cggtcagtgt aacccagaac aactacggaa ccgtctttcg
    25021 gatccaggtt gctcagctgg ctgtacagct gatccagagc agcctgacct tccggtttca
    25081 gggttgcttt gttgaaggtg aacagaacgt cagacttcag agtgaagtgc ttggtctgta
    25141 cttccggtgc cggagctgga gccggagcaa ctactggagc tacttcgccc tgaccgaaac
    25201 ggtaggaaac acccaggctc agcatgccgt tgtccggacg agtgccgatg gtgtgtgcgt
    25261 caccgatgtt gttggtccac tggtattcca gacgggtagc gatttcagga gtgatcgcat
    25321 actcaacacc gccagcgaag accggagaaa cgccggtgtc gtggttttta ccataaacgt
    25381 tggatttagt gtctgcacgc cataccatac cacccagacg agtgtatacg tccagatcgt
    25441 cagtgattgg gtaacccagt ttagcggtca gctgaacgcc ctgagcttta tatgcaccgt
    25501 tttcaacgct gcctttgtat ggcatacgac ctaaccagtc ataacccatt tcaaagccaa
    25561 catacgggtt aacctggtaa ccaccaaaag caccagcgcc cagttggttt tcatgggtcg
    25621 gaccattgtt gtcgatgaaa ccagtgtcat ggtactggga ccagcccagt ttagcaccag
    25681 tgtaccaggt gttatctttc ggagcggcct gcgctacggt agcgaaacca gccagtgcca
    25741 ctgcaatcgc gatagctgtc tttttcattt tttgcgcctc gttatcatcc aaaatacgcc
    25801 atgaatatct ccaacgagat aacacggtta aatccttcac cggggatctg ctcaatatta
    25861 actctaccga tatcttcggc ttatgccgag cacccctggc gatgtaaagt ctacaacgta
    25921 gttgaaaact tacaagtgtg aactccgtca ggcatatgaa aaaaaagtct tgtataaggt
    25981 acgtttaatc ttttttgtca gcgacaattt acagaagaaa atcgtggaaa ccgcttcaga
    26041 caagcctccg caaggaaaat tagtcacgac tgaaagcatt ggctgggcga taaaaaaagt
    26101 tccaggatta atcctaaatt tacttaatga tacaaattag agtgaatttt tagcccggaa
    26161 agttgtctcg ttgcgtgaga ggatgcgctt accggacgca taataaaccc catagcgtta
    26221 ccttcatttg ccgcatcaac aagttcagca tgctcttctt cagtcaaatc atctgccaac
    26281 caaccgatca ccacgctgta attgcccgtg cgtaaagcgc gaaccattga ctccacggtg
    26341 tggcaagggg agagctggct aatctgcatt actttcgtta agggtagccc agatgcctga
    26401 acccattcgc gactcagttt ttgttgcggt gttaaccaga gttgccagcg cgattgctga
    26461 ccgagttgct gtaacaatgg caacagtaga agttgcgtca tcatgggctg atcttcgcga
    26521 tagacaactt cactgataag cccggctgta gtgttttccg tagagacacg cgcaatttta
    26581 cttgctgcgg atgagaacga cgaagaacga tgtgcatagc ctgaagtgta cataatcaat
    26641 ccagccctgt gagttactgt atgaatatac agtacatcca gtgacgacaa agatcaactc
    26701 tattttcgga aagagcctcg caaattttgt cgttggtgac gggaaaacat aaattaatct
    26761 tgccccttaa g
//
LOCUS       DADSYM010000060        26720 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2415
            SAMN08039579-rid11723173.denovo.060, whole genome shotgun sequence.
ACCESSION   DADSYM010000060 DADSYM010000000
VERSION     DADSYM010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039579
            Sequence Read Archive: SRR6362533
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 26720)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26720)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980162.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 03:39:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,484
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 4,347
            CDSs (with protein)               :: 4,347
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 43 of 85
            Pseudo Genes (incomplete)         :: 37 of 85
            Pseudo Genes (internal stop)      :: 19 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26720
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039579-rid11723173.denovo.060"
                     /strain="LGE2415"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8302 N 95.9628 W"
                     /collection_date="2015-08-24"
     gene            1..738
                     /locus_tag="J1Y83_002099"
     CDS             1..738
                     /locus_tag="J1Y83_002099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309019.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HBA7630246.1"
                     /translation="MATSAALLTGCQNTHGINTDLAISSGLNAYKAATLSDADAKAIA
                     NQGCVEMDSGNKVASKSSKYGKRLAKIAKALGNNINGTPVNYKVYMTSDVNAWAMANG
                     CVRVYSGLMDMMNDNEIEGVLGHELGHVALGHSLAEMKASYAIVAARDAISATSGVAS
                     QLSRSQLGDIAEGAINAKYSRDKESESDDFSFDLLKKRGISTQGLVGSFEKLASLDGG
                     RTQSMFDSHPPSTERAQHIRDRIASGK"
     gene            911..1594
                     /gene="cmk"
                     /locus_tag="J1Y83_002100"
     CDS             911..1594
                     /gene="cmk"
                     /locus_tag="J1Y83_002100"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000125007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HBA7630247.1"
                     /translation="MTAIAPVITIDGPSGAGKGTLCKAMAEALQWHLLDSGAIYRVLA
                     LAALHHHVDVASEDALVPLASHLDVRFVSTNGNLEVILEGEDVSGEIRTQEVANAASQ
                     VAAFPRVREALLRRQRAFRELPGLIADGRDMGTVVFPDAPVKIFLDASSEERAHRRML
                     QLQEKGFSVNFERLLAEIKERDDRDRNRAVAPLVPAADALVLDSTTLSIEQVIEKALQ
                     YARQKLALA"
     gene            1705..3378
                     /gene="rpsA"
                     /locus_tag="J1Y83_002101"
     CDS             1705..3378
                     /gene="rpsA"
                     /locus_tag="J1Y83_002101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HBA7630248.1"
                     /translation="MTESFAQLFEESLKEIETRPGSIVRGVVVAIDKDVVLVDAGLKS
                     ESAIPAEQFKNAQGELEIQVGDEVDVALDAVEDGFGETLLSREKAKRHEAWITLEKAY
                     EDAETVTGVINGKVKGGFTVELNGIRAFLPGSLVDVRPVRDTLHLEGKELEFKVIKLD
                     QKRNNVVVSRRAVIESENSAERDQLLENLQEGMEVKGIVKNLTDYGAFVDLGGVDGLL
                     HITDMAWKRVKHPSEIVNVGDEITVKVLKFDRERTRVSLGLKQLGEDPWVAIAKRYPE
                     GTKLTGRVTNLTDYGCFVEIEEGVEGLVHVSEMDWTNKNIHPSKVVNVGDVVEVMVLD
                     IDEERRRISLGLKQCKSNPWQQFAETHNKGDRVEGKIKSITDFGIFIGLDGGIDGLVH
                     LSDISWNVAGEEAVREYKKGDEIAAVVLQVDAERERISLGVKQLAEDPFNNWVALNKK
                     GAIVTGKVTAVDAKGATVELADGVEGYLRASEASRDRVEDATLVLSVGDEVEAKFTGV
                     DRKNRAISLSVRAKDEADEKDAIATVNKQEDANFSNNAMAEAFKAAKGE"
     gene            3538..3822
                     /gene="ihfB"
                     /locus_tag="J1Y83_002102"
     CDS             3538..3822
                     /gene="ihfB"
                     /locus_tag="J1Y83_002102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit beta"
                     /protein_id="HBA7630249.1"
                     /translation="MTKSELIERLATQQSHIPAKTVEDAVKEMLEHMASTLAQGERIE
                     IRGFGSFSLHYRAPRTGRNPKTGDKVELEGKYVPHFKPGKELRDRANIYG"
     gene            4028..6292
                     /locus_tag="J1Y83_002103"
     CDS             4028..6292
                     /locus_tag="J1Y83_002103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309023.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC family protein"
                     /protein_id="HBA7630250.1"
                     /translation="MKITTVGVCIICGIFPLLILPQLPGTVTPAFLTLFACVLAFIPV
                     KTVRYIVLTLLFFVWGILAAKQILWAGETLTGATQDAIVEIIATDGMTTHYGQITHLQ
                     GRRIFPAPGLVLYGEYLPQAVCAGQVWSMKLKVRAVHGQLNDGGFDSQRYAIAQHQPL
                     TGRFLQASVIEPNCSLRAQYLASLQTTLQPYMWNAVILGLGMGERLSVPKEIKNIMRD
                     TGTAHLMAISGLHIAFAALLAAGLIRGGQVFLPGCWIHWQMPLIGGICCAAFYAWLTG
                     MQPPALRTVVALAIWGMLKLSGRQWSGWDVWICCLAAILLMDPVAILSQSLWLSAAAV
                     AALIFWYQWFPCPEWQLPPVLRAVVSLIHLQLGITLLLMPVQIVIFHGISLTSFIANL
                     FAIPLVTFITVPLILAAMVVHLSGPFILEQGLWFLADRSLALLFWGLKSLPEGWINIA
                     ERWQWLTFSPWFLLVVWRLNAWRTLPAMCVAVGLLMCLPLWQKPRPDEWQVYMLDVGQ
                     GLAMVIARNGKAILYDTGLAWPEGDSGQQLIIPWLHWHNLEPEGVILSHEHLDHRGGL
                     DSILHTWPMLWIRSPLNWEHHQPCVRGEAWQWQGLRFSAHWPLQGSNDKGNNHSCVVK
                     IDDGTNSILLTGDIEAPAEQKMLSRYWQQMQATLLQVPHHGSNTSSSLPLIQRVNGKV
                     ALASASRYNAWRLPSSKVKHRYQQQGYKWLDTPHQGQITVNFSAQGWRISSLREQILP
                     RWYHQWFGVPVDNG"
     gene            6329..8077
                     /gene="msbA"
                     /locus_tag="J1Y83_002104"
     CDS             6329..8077
                     /gene="msbA"
                     /locus_tag="J1Y83_002104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A ABC transporter ATP-binding
                     protein/permease MsbA"
                     /protein_id="HBA7630251.1"
                     /translation="MHNDKDLSTWQTFRRLWPTIAPFKAGLIVAGVALILNAASDTFM
                     LSLLKPLLDDGFGKTDRSVLMWMPLVVIGLMILRGITSYISSYCISWVSGKVVMTMRR
                     RLFGHMMGMPVSFFDKQSTGTLLSRITYDSEQVASSSSGALITVVREGASIIGLFIMM
                     FYYSWQLSIILIVLAPIVSIAIRVVSKRFRNISKNMQNTMGQVTTSAEQMLKGHKEVL
                     IFGGQEVETKRFDKVSNRMRLQGMKMVSASSISDPIIQLIASLALAFVLYAASFPSVM
                     DSLTAGTITVVFSSMIALMRPLKSLTNVNAQFQRGMAACQTLFTILDSEQEKDEGKRV
                     IERATGDVEFRNVTFTYPGRDVPALRNINLKIPAGKTVALVGRSGSGKSTIASLITRF
                     YDIDEGEILMDGHDLREYTLASLRNQVALVSQNVHLFNDTVANNIAYARTEQYSREQI
                     EEAARMAYAMDFINKMDNGLDTVIGENGVLLSGGQRQRIAIARALLRDSPILILDEAT
                     SALDTESERAIQAALDELQKNRTSLVIAHRLSTIEKADEIVVVEDGVIVERGTHNDLL
                     EHRGVYAQLHKMQFGQ"
     gene            8074..9060
                     /gene="lpxK"
                     /locus_tag="J1Y83_002105"
     CDS             8074..9060
                     /gene="lpxK"
                     /locus_tag="J1Y83_002105"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HBA7630252.1"
                     /translation="MIEKIWSGESPLWWLLLPLSWLYGLVSGAIRLCYKLKLKRAWRA
                     PVPVVVVGNLTAGGNGKTPVVVWLVEQLQQRGIRVGVVSRGYGGKAESYPLLLSADTT
                     TAQAGDEPVLIYQRTGAPVAVSPVRSDAVKAILAQHPDVQIIVTDDGLQHYRLARDVE
                     IVVIDGVRRFGNGWWLPAGPMRERAGRLKSVDAVIVNGGVPRSGEIPMHLLPGQAVNL
                     RTGTRCDVAQLEHVVAIAGIGHPPRFFATLKMCGVQPEKCVPLADHQSLNHADVSALV
                     SAGQTLVMTEKDAVKCRAFAEENWWYLPVDAQLSGDEPAKLLAQLTSLASGH"
     gene            9097..10329
                     /locus_tag="J1Y83_002106"
     CDS             9097..10329
                     /locus_tag="J1Y83_002106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HBA7630253.1"
                     /translation="MSLPHLSLADARNLHLAAQGLLNKPRRRASLEDIPATISRMSLL
                     QIDTINIVARSPYLVLFSRLGNYPAQWLDESLARGELMEYWAHEACFMPRSDFRLIRH
                     RMLAPEKMGWKYKDAWMQEHAAEIAQLIQHIHDKGPVRSADFEHPRKGASGWWEWKPH
                     KRHLEGLFTAGKVMVIERRNFQRVYDLTHRVMPEWNDERDLVSQAEAEIIMLDNSARS
                     LGIFREQWLADYYRLKRPALAAWREARAEQQQIIAVHVEKLGNLWLHADLLPLLERAL
                     AGKLTATHSAVLSPFDPVVWDRKRAEQLFDFSYRLECYIPAPKRQYGYFVLPLLHRGQ
                     LVGRMDAKMHRQTGILEVISLWLQEGIKPTTTLQKGLRQAITDFANWQQATRVTLGRC
                     PQGLFTDCRAGWEIDPVA"
     gene            10381..10563
                     /locus_tag="J1Y83_002107"
     CDS             10381..10563
                     /locus_tag="J1Y83_002107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YcaR"
                     /protein_id="HBA7630254.1"
                     /translation="MDHRLLEIIACPVCNGKLWYNQEKQELICKLDNLAFPLRDGIPV
                     LLETEARVLTADESKS"
     gene            10560..11306
                     /gene="kdsB"
                     /locus_tag="J1Y83_002108"
     CDS             10560..11306
                     /gene="kdsB"
                     /locus_tag="J1Y83_002108"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HBA7630255.1"
                     /translation="MSFVVIIPARYASTRLPGKPLVDINGKPMIVHVLERARESGAER
                     IIVATDHEDVARAVEAAGGEVCMTRADHQSGTERLAEVVEKCAFSDDTVIVNVQGDEP
                     MIPATIIRQVADNLAQRQVGMATLAVPIHNAEEAFNPNAVKVVLDAEGYALYFSRATI
                     PWDRDRFAKDLETVGDNFLRHLGIYGYRAGFIRRYVTWQPSPLEHIEMLEQLRVLWYG
                     EKIHVAVAQEVPGTGVDTPEDLKRVRAEMR"
     gene            11460..12353
                     /locus_tag="J1Y83_002109"
     CDS             11460..12353
                     /locus_tag="J1Y83_002109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcbJ family phosphotransferase"
                     /protein_id="HBA7630256.1"
                     /translation="MEQLRAELSHLLGEKLSRIECVNEKADTALWALYDSQGNPMPLM
                     ARSFSAPGKARQLAWKTTMLARSGTVRMPTIYGVMTHEEHLGPDVLLLERMRGVSVEA
                     PARTPERWEQLKDQIVEALLAWHRQDSRGCVGAVDNTQENFWPSWYRQHVEVLWTTLN
                     QFNNTGLTMQDKRILFRTRECLPALFEGFNDNCVLIHGNFCLRSMLKDSRSDQLLAMV
                     GPGLMLWAPREYELFRLMDNSLAEDLLWSYLQRAPVAESFIWRRWLYVLWDEVAQLVN
                     TGRFSRRNFDLASKSLLPWLA"
     gene            complement(12330..13109)
                     /gene="elyC"
                     /locus_tag="J1Y83_002110"
     CDS             complement(12330..13109)
                     /gene="elyC"
                     /locus_tag="J1Y83_002110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope biogenesis factor ElyC"
                     /protein_id="HBA7630257.1"
                     /translation="MLFTLKKVIGNMLLPLPLMLLIIGAGLALLWFSRFQKTGKIFIS
                     IGWLALLLLSLQPIADRLLRPIESTYPTWNNSQKVDYIVVLGGGYTWNPQWAPSSNLI
                     NNSLPRLNEGIRLWRENPGSKLIFTGGVAKTNTVSTAEVGARVAQSLGVPREQIITLD
                     LPKDTEEEAAAVKQAIGDAPFLLVTSASHLPRAMIFFQQEGLNPLPAPANQLAIDSPL
                     NPWERAIPSPVWLMHSDRVGYETLGRIWQWLKGSSGEPRQE"
     gene            13245..14030
                     /gene="cmoM"
                     /locus_tag="J1Y83_002111"
     CDS             13245..14030
                     /gene="cmoM"
                     /locus_tag="J1Y83_002111"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine 5-oxyacetic acid(34)
                     methyltransferase CmoM"
                     /protein_id="HBA7630258.1"
                     /translation="MQDRNFDDIAEKFSRNIYGTTKGQLRQAILWQDLDRVLAEMGPQ
                     KLRVLDAGGGEGQTAIKMAERGHQVILCDLSAQMIDRAKQAAEAKGVSDNMQFIHCAA
                     QDVASHLETPVDLILFHAVLEWVADPRSVLQTLWSVLRPGGVLSLMFYNAHGLLMHNM
                     VAGNFDYVQAGMPKKKKRTLSPDYPRDPAQVYLWLEEAGWQIMGKTGVRVFHDYLREK
                     HQQRDCYEALLELETRYCRQEPYITLGRYIHVTARKPQSKDKV"
     gene            14027..15349
                     /gene="mukF"
                     /locus_tag="J1Y83_002112"
     CDS             14027..15349
                     /gene="mukF"
                     /locus_tag="J1Y83_002112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukF"
                     /protein_id="HBA7630259.1"
                     /translation="MSEFSQTVPELVAWARKNDFSISLPVDRLSFLLAVATLNGERLD
                     GEMSEGELVDAFRHVSDAFEQTSETIGVRANNAINDMVRQRLLNRFTSEQAEGNAIYR
                     LTPLGIGITDYYIRQREFSTLRLSMQLSIVAGELKRAADAAEEGGDEFHWHRNVYAPL
                     KYSVAEIFDSIDLTQRLMDEQQQQVKDDIAQLLNKDWRAAISSCELLLSETSGTLREL
                     QDTLEAAGDKLQANLLRIQDATMTHDDLHFVDRLVFDLQSKLDRIISWGQQSIDLWIG
                     YDRHVHKFIRTAIDMDKNRVFAQRLRQSVQTYFDEPWALTYANADRLLDMRDEEMALR
                     DEEVTGELPEDLEYEEFNEIREQLAAIIEEQLAVYKTRQVPLDLGLVVREYLSQYPRA
                     RHFDVARIVIDQAVRLGVAQADFTGLPAKWQPINDYGAKVQAHVIDKY"
     gene            15330..16034
                     /gene="mukE"
                     /locus_tag="J1Y83_002113"
     CDS             15330..16034
                     /gene="mukE"
                     /locus_tag="J1Y83_002113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukE"
                     /protein_id="HBA7630260.1"
                     /translation="MSSTNIEQVMPVKLAQALANPLFPALDSALRSGRHIGLDELDNH
                     AFLMDFQEYLEEFYARYNVELIRAPEGFFYLRPRSTTLIPRSVLSELDMMVGKILCYL
                     YLSPERLANEGIFTQQELYDELLTLADEAKLLKLVNNRSTGSDVDRQKLQEKVRSSLN
                     RLRRLGMVWFMGHDSSKFRITESVFRFGADVRAGDDPREAQRRLIRDGEAMPIENHLQ
                     LNDETEESQPDSGEEE"
     gene            16034..20494
                     /gene="mukB"
                     /locus_tag="J1Y83_002114"
     CDS             16034..20494
                     /gene="mukB"
                     /locus_tag="J1Y83_002114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukB"
                     /protein_id="HBA7630261.1"
                     /translation="MIERGKFRSLTLINWNGFFARTFDLDELVTTLSGGNGAGKSTTM
                     AAFVTALIPDLTLLHFRNTTEAGATSGSRDKGLHGKLKAGVCYSMLDTINSRHQRVVV
                     GVRLQQVAGRDRKVDIKPFAIQGLPMSVQPTQLVTETLNERQARVLPLNELKDKLEAM
                     EGVQFKQFNSITDYHSLMFDLGIIARRLRSASDRSKFYRLIEASLYGGISSAITRSLR
                     DYLLPENSGVRKAFQDMEAALRENRMTLEAIRVTQSDRDLFKHLISEATNYVAADYMR
                     HANERRVHLDKALEFRRELHTSRQQLAAEQYKHVDMARELAEHNGAEGDLEADYQAAS
                     DHLNLVQTALRQQEKIERYEADLDELQIRLEEQNEVVAEAIERQEENEARAEAAELEV
                     DELKSQLADYQQALDVQQTRAIQYNQAIAALNRAKELCYLPDLTADSAAEWLETFQAK
                     ELEATEKMLSLEQKMSMAQTAHSQFEQAYQLVVAINGPLARNEAWDVARELLREGVDQ
                     RHLAEQVQPLRMRLSELEQRLREQQEAERLLADFCKRQGKNFDIDELEALHQELEARI
                     ASLSDSVSNAREERMALRQEQEQLQSRIQSLMQRAPVWLAAQNSLNQLSEQCGEEFTS
                     SQDVTEFLQQLLEREREAIVERDEVGARKNAVDEEIERLSQPGGSEDQRLNALAERFG
                     GVLLSEIYDDVSLEDAPYFSALYGPSRHAIVVPDLSQVTEHLEGLTDCPEDLYLIEGD
                     PQSFDDSVFSVDELEKAVVVKIADRQWRYSRFPEVPLFGRAARESRIESLHAEREVLS
                     ERFATLSFDVQKTQRLHQAFSRFIGSHLAVAFESDPEAEIRQLNSRRVELERALSNHE
                     NDNQQQRIQFEQAKEGVTALNRILPRLNLLADDSLADRVDEIRERLDEAQEAARFVQQ
                     FGNQLAKLEPIVSVLQSDPEQFEQLKEDYAYSQQMQRDARQQAFALTEVVQRRAHFSY
                     SDSAEMLSGNSDLNEKLRERLEQAEAERTRAREALRGHAAQLNQYNQVLASLKSSYDT
                     KKELLNDLQRELQDIGVRADSGAEERARIRRDELHAQLSNNRSRRNQLEKALTFCEAE
                     MDNLTRKLRKLERDYFEMREQVVTAKAGWCAVMRMVKDNGVERRLHRRELAYLSADDL
                     RSMSDKALGALRLAVADNEHLRDVLRMSEDPKRPERKIQFFVAVYQHLRERIRQDIIR
                     TDDPVEAIEQMEIELSRLTEELTSREQKLAISSRSVANIIRKTIQREQNRIRMLNQGL
                     QNVSFGQVNSVRLNVNVRETHAMLLDVLSEQHEQHQDLFNSNRLTFSEALAKLYQRLN
                     PQIDMGQRTPQTIGEELLDYRNYLEMEVEVNRGSDGWLRAESGALSTGEAIGTGMSIL
                     VMVVQSWEDESRRLRGKDISPCRLLFLDEAARLDARSIATLFELCERLQMQLIIAAPE
                     NISPEKGTTYKLVRKVFQNTEHVHVVGLRGFAPQLPETLPGSDEAPSQAS"
     gene            20755..22602
                     /gene="ldtD"
                     /locus_tag="J1Y83_002115"
     CDS             20755..22602
                     /gene="ldtD"
                     /locus_tag="J1Y83_002115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HBA7630262.1"
                     /translation="MLLNMMCGRRLSAISLCLAVTFAPLFNAQADEPEVIPGDSPVAV
                     SEQGEALPQAQATAIMAGIQPLPEGAAEKARTQIESQLPAGYKPVYLNQLQLLYAARD
                     MQPMWENRDAVKAFQQQLAEVAIAGFQPQFNKWVELLTDPGVNGMTRDVVLSDAMMGY
                     LHFIANIPVKGTRWLYSSKPYALATPPLSVINQWQLALDKGQLPTFVAGLALQHPQYA
                     AMHESLLALLSDTKPWPQLTGKATLRPGQWSNDVPALREILQRTGMLDGGPKITLPGD
                     DTPTDAVVSPSAVTIETAETKPMDKQTTSRSKPAPVVRAAYDNELVEAVKRFQTWQGL
                     GADGAIGPATRDWLNVTPAQRAGVLALNIQRLRLLPTELSTGIMVNIPAYSLVYYQNG
                     NQVLDSRVIVGRPDRKTPMMSSALNNVVVNPPWNVPPTLARKDILPKVRNDPGYLESH
                     GYTVMRGWNSREAIDPWQVDWSTITASNLPFRFQQAPGPRNSLGRYKFNMPSSEAIYL
                     HDTPNHNLFKRDTRALSSGCVRVNKASDLANMLLQDAGWNDKRISDALKQGDTRYVNI
                     RQSIPVNLYYLTAFVGADGRTQYRTDIYNYDLPARSSSQIVSKAEQLIR"
     gene            22783..23331
                     /locus_tag="J1Y83_002116"
     CDS             22783..23331
                     /locus_tag="J1Y83_002116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcbK family protein"
                     /protein_id="HBA7630263.1"
                     /translation="MDKFDANRRKLLALGGVALGAAILPTPAFATLSTPRPRILTLNN
                     LHTGESIKAEFFDGRGYIQEELAKLNHFFRDYRANKIKSIDPGLFDQLYRLQGLLGTR
                     KPVQLISGYRSIDTNNELRARSRGVAKKSYHTKGQAMDFHIEGIALSNIRKAALSMRA
                     GGVGYYPRSNFVHIDTGPARHW"
     gene            23358..24005
                     /gene="gloC"
                     /locus_tag="J1Y83_002117"
     CDS             23358..24005
                     /gene="gloC"
                     /locus_tag="J1Y83_002117"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003035823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase GloC"
                     /protein_id="HBA7630264.1"
                     /translation="MNYRIIPVTAFSQNCSLIWCEQTRLAALVDPGGDAEKIKQEVDA
                     SGLTLMQILLTHGHLDHVGAAAELAQHYGVPVFGPEKEDEFWLQGLPAQSRMFGLEEC
                     QPLTPDRWLNEGDTISIGNVTLQVLHCPGHTPGHVVFFDDRAKLLISGDVIFKGGVGR
                     SDFPRGDHNQLISSIKDKLLPLGDDVTFIPGHGPLSTLGYERLHNPFLQDEMPVW"
     gene            complement(24055..25245)
                     /locus_tag="J1Y83_002118"
     CDS             complement(24055..25245)
                     /locus_tag="J1Y83_002118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009653304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate/tyrosine/aromatic aminotransferase"
                     /protein_id="HBA7630265.1"
                     /translation="MFENITAAPADPILGLADLFRADERPGKINLGIGVYKDETGKTP
                     VLTSVKKAEQYLLENETTKNYLGIDGIPEFGRCTQELLFGKGSALINDKRARTAQTPG
                     GTGALRVAADFLAKNTSVKRVWVSNPSWPNHKSVFNSAGLEVREYAYYDAENHTLDFD
                     ALINSLNEAQAGDVVLFHGCCHNPTGIDPTLEQWQTLAQLSVEKGWLPLFDFAYQGFA
                     RGLEEDAEGLRAFAAMHKELIVASSYSKNFGLYNERVGACTLVAADSETVDRAFSQMK
                     AAIRANYSNPPAHGASVVATILSNDALRAIWEQELTDMRQRIQRMRQLFVNTLQEKGA
                     NRDFSFIIKQNGMFSFSGLTKEQVLRLREEFGVYAVASGRVNVAGMTPDNMAPLCEAI
                     VAVL"
     gene            complement(25430..26518)
                     /gene="ompF"
                     /locus_tag="J1Y83_002119"
     CDS             complement(25430..26518)
                     /gene="ompF"
                     /locus_tag="J1Y83_002119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpF"
                     /protein_id="HBA7630266.1"
                     /translation="MMKRNILAVIVPALLVAGTANAAEIYNKDGNKVDLYGKAVGLHY
                     FSKGNGENSYGGNGDMTYARLGFKGETQINSDLTGYGQWEYNFQGNNSEGADAQTGNK
                     TRLAFAGLKYADVGSFDYGRNYGVVYDALGYTDMLPEFGGDTAYSDDFFVGRVGGVAT
                     YRNSNFFGLVDGLNFAVQYLGKNERDTARRSNGDGVGGSISYEYEGFGIVGAYGAADR
                     TNLQEAQPLGNGKKAEQWATGLKYDANNIYLAANYGETRNATPITNKFTNISGFANKT
                     QDVLLVAQYQFDFGLRPSIAYTKSKAKDVEGIGDVDLVNYFEVGATYYFNKNMSTYVD
                     YIINQIDSDNKLGVGSDDTVAVGIVYQF"
BASE COUNT         6503 a         6522 c         7236 g         6459 t
ORIGIN      
        1 attgcgacct ctgcagcctt actgacagga tgtcagaata cgcacggtat taataccgat
       61 ttggctatca gctcaggtct gaatgcttat aaagccgcaa cattaagcga tgctgatgct
      121 aaagcaatcg ccaatcaggg ctgtgtcgaa atggacagcg gcaataaagt ggcaagcaaa
      181 tccagcaagt acgggaaacg tctggcaaaa atcgccaaag cattgggtaa caatattaat
      241 ggcacgccag tcaactataa ggtttatatg accagcgacg tcaacgcatg ggcgatggcg
      301 aacggttgtg ttcgtgtcta cagtggcctg atggacatga tgaacgacaa cgaaattgaa
      361 ggcgttctgg gccatgaact gggccacgtc gcgttgggtc actcgctggc tgaaatgaaa
      421 gcttcttatg cgatcgtggc cgcacgcgat gccatttcag ctaccagcgg tgtggcttcc
      481 cagctttccc gttcacaatt aggtgatatc gcagaaggcg ctatcaatgc taaatactcc
      541 cgtgataaag aatccgaatc agatgatttc tcttttgatc tgttgaagaa acgtggcatc
      601 agtacccagg ggctggttgg tagctttgaa aaactggcca gcctggatgg cggtcgcacc
      661 cagtccatgt ttgattctca cccaccatca acagagcgtg cgcaacacat ccgtgatcgt
      721 atcgcctctg gtaagtaaat cattgtcatc tttcgggctg gtcttctgcc agcccgctat
      781 aattgcgcaa taaatcccca tctgaataca gacaaaactg gtttttgcac acaacgttaa
      841 cgatttgtgg cgtcggcgcg tataatgcgc gcggttatgt taacggtacg cctgttttaa
      901 ggagataaag atgacggcaa ttgccccggt tattaccatt gatggcccaa gcggtgcagg
      961 gaaaggcacc ttgtgtaagg ctatggcgga agcgttgcaa tggcatctgc tggattcggg
     1021 tgcaatttat cgcgtactgg cactagcggc attacatcac catgttgatg ttgcatcgga
     1081 agatgcgctg gtgccgctgg catcccatct ggatgtacgt tttgtgtcaa ccaacggcaa
     1141 tctggaagtg atcctcgaag gggaagatgt cagcggcgaa attcgtactc aggaggtggc
     1201 gaatgctgct tcacaagtcg cggcattccc acgcgttcgt gaagcattat tgcgtcgcca
     1261 acgcgcgttt cgcgaattac caggcctgat tgccgatggg cgcgacatgg gaacggtggt
     1321 attccctgat gcgccagtga aaattttcct tgacgcctcc tcggaagaac gtgcgcatcg
     1381 ccgcatgcta cagttgcagg agaagggctt tagtgttaac tttgagcgcc ttttggccga
     1441 gatcaaagaa cgcgacgacc gcgatcgtaa ccgagcggta gcgccactgg ttccggcagc
     1501 cgatgcttta gtgttggatt ccaccacctt aagcattgag caagtgattg aaaaagcgct
     1561 acaatacgcg cgccagaaat tggctctcgc ataagcgacc gaatttgcag tacccccgtt
     1621 gcaatggaat gacagcgggt atgttaaaca accccatccg gcatggagcc aggtggacgt
     1681 taaatataaa cctgaagatt aaacatgact gaatcttttg ctcaactctt tgaagagtcc
     1741 ttaaaagaaa tcgaaacccg cccgggttct atcgttcgtg gcgttgttgt tgctatcgac
     1801 aaagacgtag tactggttga cgctggtctg aaatctgagt ccgccatccc ggctgagcag
     1861 ttcaaaaacg cccagggcga gctggaaatc caggtaggtg acgaagttga cgttgctctg
     1921 gacgcagtag aagacggctt cggtgaaact ctgctgtccc gtgagaaagc taaacgtcac
     1981 gaagcctgga tcacgctgga aaaagcttac gaagatgctg aaactgttac cggtgttatc
     2041 aacggcaaag tcaagggcgg cttcactgtt gagctgaacg gtattcgtgc gttcctgcca
     2101 ggttctctgg tagacgttcg tccggttcgt gacaccctgc acctggaagg caaagagctt
     2161 gaattcaaag taatcaagct ggaccagaag cgtaacaacg ttgttgtttc tcgccgtgct
     2221 gttattgagt ctgaaaacag tgcagaacgc gatcagctgc tggaaaacct gcaggaaggc
     2281 atggaagtta aaggtatcgt taagaacctc actgactacg gtgcattcgt tgatctgggt
     2341 ggtgttgacg gtctgctgca catcaccgac atggcgtgga aacgcgttaa acatccgagc
     2401 gaaattgtaa acgttggcga cgaaatcact gttaaagtgc tgaagttcga ccgcgaacgt
     2461 acccgtgtat ctctgggcct gaaacaactg ggcgaagatc cgtgggtagc tatcgctaaa
     2521 cgttatccgg aaggtaccaa actgaccggt cgcgtgacca acctgaccga ctacggctgc
     2581 ttcgttgaaa tcgaagaagg cgttgaaggt ctggtacacg tttccgaaat ggattggacc
     2641 aacaaaaaca tccacccgtc caaagttgtt aacgttggcg atgtagtgga agttatggtt
     2701 ctggatatcg acgaagaacg tcgtcgtatc tccctgggtc tgaagcagtg caaatctaac
     2761 ccatggcagc agttcgcgga aacccacaac aagggcgacc gtgttgaagg taaaatcaag
     2821 tctatcactg acttcggtat cttcatcggc ctggacggcg gtatcgacgg cttggttcac
     2881 ctgtctgaca tctcctggaa cgttgcaggc gaagaagcag ttcgtgaata caaaaaaggc
     2941 gacgaaatcg cagcagttgt actgcaggtt gacgcagaac gtgaacgtat ctccctgggc
     3001 gttaagcagc tcgctgaaga tccgttcaac aactgggttg ctctgaacaa gaaaggcgct
     3061 atcgtaaccg gtaaagtaac tgcagttgac gctaaaggcg caaccgtaga actggctgac
     3121 ggcgttgaag gttacctgcg tgcttctgaa gcatcccgtg accgcgttga agacgctacc
     3181 ctggttctga gcgttggcga cgaagttgaa gctaaattca ccggcgttga tcgtaaaaac
     3241 cgcgcaatca gcctgtctgt tcgtgcgaaa gacgaagctg acgagaaaga tgcaatcgca
     3301 actgttaaca aacaggaaga tgcaaacttc tccaacaacg caatggctga agctttcaaa
     3361 gcagctaaag gcgagtaatt ctctgactct tcgggatttt tattccgaag tttgttgagt
     3421 ttacttgaca gattgcaggt ttcgtcctgt aatcaagcac taagggcggc tacggccgcc
     3481 cttaatcaat gcagcaacag cagccgctta atttgccttt aaggaaccgg aggaatcatg
     3541 accaagtcag aattgataga aagacttgcc acccagcaat cgcacattcc cgccaagacg
     3601 gttgaagatg cagtaaaaga gatgctggag catatggcct cgactcttgc gcagggcgag
     3661 cgtattgaaa tccgcggttt cggcagtttc tctttgcact accgcgcacc acgtaccgga
     3721 cgtaatccga agactggcga taaagtagaa ctggaaggaa aatacgttcc tcactttaaa
     3781 ccaggtaaag aactgcgcga tcgcgccaat atttacggtt aagtttttac tcaaacttga
     3841 acgcgagaaa agcacctgtc gggtgctttt ttcatttctc taatctggaa ctggaagctg
     3901 cctcgcagag ttttgagcag ttttcaccct atcgttaaat tcttctgaac gtgcctcggg
     3961 gaacgcaaaa ctcccaccag acaaccgttc aacaaagttg cacactttct ataaacaggg
     4021 agggtgcatg aaaataacga cagtcggcgt atgcataatt tgcggaattt ttccgttgct
     4081 gattttgccc caattgcctg ggacagtaac ccctgcgttt ctgactctct tcgcctgtgt
     4141 actggcattt atccctgtta aaaccgtccg ttatatcgtg ctgacgttgc tgtttttcgt
     4201 ttggggcata ttagcagcaa agcaaatttt gtgggcagga gaaaccttaa ctggcgcgac
     4261 gcaggatgca attgttgaga tcattgctac tgacggcatg accactcatt acggtcaaat
     4321 tactcatcta caaggtcgac gtatattccc tgcgccaggc ctcgtactgt atggcgaata
     4381 tcttccgcaa gcggtttgtg ccggacaagt atggtcaatg aaactcaaag ttcgtgcagt
     4441 tcatggtcaa cttaatgatg gcggctttga tagccagcgt tatgccattg cccagcatca
     4501 accgctcacc ggccgttttc tgcaggcaag tgtcattgaa ccgaattgta gcctgcgtgc
     4561 acagtatctg gcgtcactac aaacaacgct gcaaccctat atgtggaacg cggttattct
     4621 tggattaggt atgggggaac ggttatccgt ccccaaagaa atcaaaaata tcatgcgtga
     4681 tactggaacg gcgcatttaa tggcgatatc aggattgcac atcgcttttg cggcgttgct
     4741 ggctgcagga ctcattcgcg gtgggcaagt ttttctgcct gggtgctgga tccactggca
     4801 aatgccatta attggtggaa tctgctgtgc tgctttttat gcctggctta cgggaatgca
     4861 acctcctgca ttgcgtaccg tggtggcgct tgccatttgg ggaatgctta agttaagtgg
     4921 acgacaatgg agtggctggg atgtatggat atgttgtctg gcggcaattt tgctgatgga
     4981 tcctgttgcc attctctcgc aaagtttatg gctctctgcc gctgcggtcg cggcactgat
     5041 tttttggtat cagtggtttc cctgtccgga gtggcagctg ccgccggtat tgcgcgcggt
     5101 tgtttccctc atccatctgc aactgggaat caccctcctg cttatgcccg tgcaaattgt
     5161 catatttcat ggcattagtc tgacctcgtt tattgcaaat ctttttgcaa ttcccctggt
     5221 gacatttatc acggttccgt tgattctcgc cgcgatggtt gtgcatttaa gcgggccgtt
     5281 tattctggaa caagggttat ggtttcttgc cgaccggtct ttggctttac ttttctgggg
     5341 gttaaagagt ttgccggaag ggtggatcaa cattgctgaa cgttggcaat ggctaacatt
     5401 ttccccatgg ttcttactgg tggtatggcg attaaacgcc tggcgaacgt tgccagcaat
     5461 gtgtgtggct gtaggcttgc tgatgtgctt gccgctgtgg caaaaacctc gacctgacga
     5521 gtggcaagtg tacatgcttg atgtcgggca agggctggca atggtcattg cgcgaaacgg
     5581 caaagcgatt ctctatgaca cgggactggc ctggcccgaa ggggatagtg ggcaacaact
     5641 gattatcccc tggctccact ggcataatct tgaaccggaa ggcgttattt tgagtcatga
     5701 acatctggat caccggggag ggctggactc aatattgcac acatggccga tgttatggat
     5761 ccgaagtccg ttaaactggg aacatcatca gccctgtgtg cgtggcgaag cgtggcaatg
     5821 gcaaggattg cgtttcagcg cgcactggcc tttacaaggt agcaacgata aaggaaataa
     5881 ccattcctgt gtggttaaga ttgatgacgg gacgaatagc attcttctaa ccggtgatat
     5941 tgaagccccc gctgaacaaa agatgctaag ccgttactgg cagcaaatgc aggcaacatt
     6001 acttcaggta cctcaccatg gcagtaatac ctcatcatcg ttgccattaa ttcagcgagt
     6061 gaatggaaaa gtggcactcg catcggcatc gcgctataac gcatggcgac tgccctcaag
     6121 caaagttaaa catcgctatc aacaacaggg ttataagtgg cttgatactc cacatcaggg
     6181 tcaaataacg gtcaattttt cagcgcaagg ctggcggatt agcagcctca gagagcaaat
     6241 tttacctcgt tggtatcatc agtggtttgg cgtgccagtg gataacgggt agaatatgcg
     6301 gctatttcaa caaatgctgg ttttttgaat gcataacgac aaagatctct ctacgtggca
     6361 gacgttccgc cgactgtggc caaccattgc gccttttaaa gcgggtctga tcgtggcggg
     6421 cgtagcgtta atcctcaacg cagccagcga taccttcatg ttatcgctcc ttaagccact
     6481 tcttgatgat ggctttggta aaacagatcg ctccgtgctg atgtggatgc cgctggtggt
     6541 gatcgggctg atgattttac gtggtatcac cagctatatc tccagctact gtatctcctg
     6601 ggtatcagga aaggtggtaa tgactatgcg tcgccgcctg tttggtcaca tgatgggaat
     6661 gccggtttca ttctttgaca aacagtcaac gggtacgctg ttgtcacgta tcacctacga
     6721 ttccgaacag gtcgcttctt cttcttccgg cgcactgatt actgttgtgc gtgaaggtgc
     6781 gtcgatcatc ggcctgttca tcatgatgtt ctattacagt tggcaactgt cgatcatttt
     6841 gattgtgctg gcaccgattg tttcgattgc gattcgcgtt gtatcgaagc gttttcgcaa
     6901 catcagtaaa aacatgcaga acaccatggg gcaggtgacc accagcgccg aacaaatgct
     6961 gaaaggccat aaagaagtat tgattttcgg tggtcaggaa gtggaaacga aacgcttcga
     7021 taaagtcagt aaccgaatgc gtcttcaggg gatgaaaatg gtttcagcct cttctatctc
     7081 tgatccgatc attcagctga tcgcctcttt ggcgctggcg tttgttctgt atgcagcaag
     7141 cttcccaagt gtcatggata gcctgactgc cggtacgatt accgttgttt tctcttcaat
     7201 gattgcactt atgcgtccgc tgaaatcgct gaccaacgtt aacgcccagt tccagcgtgg
     7261 tatggcggct tgtcagacgc tgtttaccat tctggacagt gagcaggaga aagacgaagg
     7321 taagcgcgtg atcgagcgtg caactggcga cgtggaattt cgcaatgtca cctttactta
     7381 tccaggacgt gacgtgcccg cattgcgtaa catcaacctg aaaattccgg cagggaagac
     7441 ggttgctctg gttggacgat ctggttcagg taaatcaacc atcgccagcc taatcacacg
     7501 tttttacgat attgatgaag gcgaaatcct gatggatggt cacgatctgc gcgagtatac
     7561 cctggcgtcg ttacgtaacc aggttgctct ggtgtcgcag aatgtccatc tgtttaacga
     7621 tacggttgcc aacaacattg cttacgcaag gactgaacag tacagccgtg agcaaattga
     7681 agaagctgcg cgtatggcct acgccatgga cttcatcaat aagatggata acggtctcga
     7741 tacagtcatt ggtgaaaacg gcgtgctgct ctctggcggt cagcgtcagc gtattgctat
     7801 cgctcgagcc ttgttgcgtg atagcccgat actgattctg gacgaagcta cctcggcgtt
     7861 ggataccgaa tccgaacgtg cgattcaggc ggcactggat gagttgcaga aaaaccgtac
     7921 ctctctggtg attgcccacc gcttgtctac cattgaaaag gcagacgaaa tcgtggttgt
     7981 cgaggatggt gtcattgtgg aacgcggtac gcataacgat ttgcttgagc accgtggcgt
     8041 ttacgcgcaa cttcacaaaa tgcagtttgg ccaatgatcg aaaaaatctg gtctggtgaa
     8101 tcccctttat ggtggctatt gctgccactc tcctggttgt atggcctggt gagtggcgcg
     8161 atccgtcttt gctataaact aaaactgaag cgcgcctggc gtgcccccgt accggttgtc
     8221 gtggttggta atctcaccgc aggcggcaac ggaaaaaccc cggtcgttgt ctggctggtg
     8281 gaacagctgc aacagcgcgg tattcgcgtg ggggtcgtat cgcggggata tggtggtaag
     8341 gctgaatctt atccgctgtt attgtcggca gataccacca cagcacaggc gggtgatgaa
     8401 cctgtgttga tttatcaacg cactggcgcg cctgttgcgg tttctcccgt gcgttctgac
     8461 gcggtaaaag ccattctggc gcaacaccct gatgtgcaga tcatcgtaac cgacgatggt
     8521 ttacagcatt atcgtctggc gcgtgatgtg gaaattgtcg ttattgatgg tgtgcgtcgc
     8581 tttggcaatg gctggtggtt gccggcgggg ccaatgcgtg agcgagcggg gcgcttaaag
     8641 tcggttgatg cggtaatcgt caacggcggt gtcccccgca gcggtgaaat ccccatgcat
     8701 ctgctgccgg gtcaggcggt gaatttacgt accggtacgc gttgtgacgt tgctcagctt
     8761 gaacatgtgg tggcgattgc ggggattggg catccgccgc gcttttttgc cacgctgaag
     8821 atgtgcggcg tacaaccgga aaaatgtgta ccgctggccg atcatcagtc tttgaatcat
     8881 gcggatgtca gcgcgttggt aagcgccgga caaacgctgg taatgactga aaaagatgcg
     8941 gtgaaatgcc gggcctttgc agaagaaaat tggtggtatt tgcctgtaga cgcacagctt
     9001 tcaggtgatg aaccagcgaa actgcttgcg caactaacct cgctggcttc tggccactag
     9061 ttacgccgcg gcagcgttcg attgatggag tcatgaatgt cgctgccgca cctctccctt
     9121 gctgatgcgc gtaatcttca ccttgccgcg caaggcctgt taaacaaacc ccgccgtcga
     9181 gcgtcgttgg aggatattcc ggcaacgatc tcccgcatgt ccttgctgca aatcgatacc
     9241 atcaatattg ttgcccgtag tccatatctg gtgcttttca gtcgtctggg aaattatcct
     9301 gcccagtggc tggatgagtc tctggcgcgt ggcgaattaa tggaatactg ggcgcatgaa
     9361 gcctgcttta tgccgcgtag cgattttcgt cttattcgcc accgcatgct ggcacctgaa
     9421 aaaatgggct ggaaatataa agacgcctgg atgcaggaac atgcggcgga aattgcacag
     9481 ttaattcagc atattcatga taaggggcca gtacgttcag ccgattttga gcatcctcgt
     9541 aaaggggcga gcggctggtg ggagtggaag ccgcataaac gacatctgga aggtttattt
     9601 actgccggaa aggtgatggt gattgaacgg cgcaacttcc agcgcgttta tgatttaact
     9661 caccgtgtca tgcctgagtg gaatgatgag cgcgatctcg tttcgcaagc agaagcagaa
     9721 atcatcatgc tggataacag tgcgcgtagc ctgggaatat ttcgcgaaca gtggctggca
     9781 gattactacc ggctaaaacg tccggcactg gcggcgtggc gtgaagcgag ggctgaacag
     9841 cagcaaatca ttgctgtgca tgttgaaaaa ttgggcaatc tttggctgca tgctgatttg
     9901 ctgccgctac tcgagcgtgc gctcgccgga aagctcactg caacgcacag cgcggtactt
     9961 tcgccttttg atcctgttgt ctgggatcgc aaacgcgcgg agcagctttt tgattttagc
    10021 taccggctgg agtgctatat cccagcgccg aaacgccagt atggctattt tgttctgccg
    10081 ttattacatc gtgggcaatt agttgggcga atggatgcca aaatgcatcg ccagacaggc
    10141 atccttgaag ttatctctct gtggttacag gaaggcatta aaccaacgac aacgctgcaa
    10201 aaagggttac gtcaggcgat tactgatttc gctaactggc agcaggcaac gcgggtgaca
    10261 ttaggacgct gcccgcaagg cctctttact gattgccgcg ccggctggga aatagacccc
    10321 gtcgcataaa tgattatgat aagatttatg cattcataac ccgatccatc tggaggaact
    10381 atggatcatc gtctgcttga aatcattgcc tgcccggttt gcaacggaaa actttggtat
    10441 aaccaggaaa aacaagagct tatttgcaaa ctggacaacc tggctttccc gctgcgcgat
    10501 ggcattccag tgttgctgga aacggaagcc cgcgtgctga ctgctgatga gagtaaatca
    10561 tgagttttgt ggtcattatt cccgcgcgtt acgcgtccac gcgtctgccc ggtaaaccat
    10621 tggttgatat taacggcaaa cccatgattg ttcatgttct tgaacgcgcg cgcgaatcag
    10681 gtgccgagcg catcatcgtg gcaaccgatc atgaggatgt tgcccgcgct gttgaagccg
    10741 ctggcggcga agtatgtatg acgcgcgccg atcatcagtc aggtacagaa cgtctggcgg
    10801 aagttgtcga aaaatgcgca ttcagcgacg acactgttat cgttaatgtg cagggtgatg
    10861 aaccgatgat ccctgcgaca atcattcgtc aggttgctga taacctcgct cagcgtcagg
    10921 tgggtatggc gactctggcg gtgccaatcc acaatgcgga agaagcgttt aacccgaatg
    10981 cggtgaaagt ggttctcgac gccgaagggt acgcactgta cttctctcgc gccaccattc
    11041 cgtgggatcg tgatcgtttt gcaaaagacc ttgaaaccgt aggcgataac ttcctgcgtc
    11101 atcttggtat ttatggctac cgagcaggct ttatccgtcg ttatgttacc tggcagccaa
    11161 gtccgttaga acatatcgaa atgttagagc agcttcgtgt tctgtggtac ggcgaaaaaa
    11221 tccacgttgc tgtcgcccag gaagttcctg gcacaggtgt ggatacccct gaagatctca
    11281 agcgcgttcg cgccgaaatg cgctaatttc acttcacgac actccagcca attttgggag
    11341 gagtgtcgta ccgttacgat tttcctcaat ttttcttttc aacaattgat ctcattcagg
    11401 tgacatcttt tatattggcg ctcattatga aagcagtagc ttttatgagg gtaatctgaa
    11461 tggaacagct gcgtgccgaa ttaagccatt tactgggcga aaaactcagt cgtattgagt
    11521 gcgtcaatga aaaagcggat acggcgttgt gggctttgta tgacagccag ggaaacccaa
    11581 tgccgttaat ggcaagaagc tttagtgcgc ccggaaaagc ccgacaactg gcatggaaaa
    11641 ccaccatgct ggcaagaagt gggactgtcc gtatgccgac tatttatggt gtgatgacgc
    11701 atgaagaaca cctcggcccc gatgtcctgc tactggagcg aatgcgtggt gtttcggtgg
    11761 aggcaccagc ccgaacacca gaacgctggg aacaactcaa agatcaaatc gttgaagcct
    11821 tactggcctg gcaccgtcag gacagtcgtg gttgcgtcgg cgcggtcgac aatactcagg
    11881 aaaatttctg gccctcatgg taccggcaac atgttgaagt gctatggacc acgctcaatc
    11941 agttcaataa caccggtcta acgatgcagg ataagcggat cctgtttcgc actcgcgaat
    12001 gtctcccggc attatttgaa ggctttaacg acaattgtgt actgattcac ggtaacttct
    12061 gtttacgtag catgctgaaa gattcgcgca gcgatcagtt actggcgatg gtcggaccgg
    12121 gactaatgct ttgggcaccg cgagaatacg aactgttccg actaatggat aattctctgg
    12181 cggaagattt gctctggagt tatctgcaac gcgcgccagt ggcggagtcg ttcatctggc
    12241 ggcgttggtt gtatgtgtta tgggatgaag ttgcgcaact ggtcaacacc ggacgattta
    12301 gtcggcgtaa cttcgatctg gcatcaaaat cactcttgcc gtggctcgcc tgacgaacct
    12361 ttcagccact gccagatccg gccaagcgtt tcatagccaa cgcggtcgct atgcatcaac
    12421 caaaccggag aagggattgc ccgttcccac ggattaagcg gcgagtcgat ggccaactga
    12481 ttcgccgggg ctggtagcgg atttaaccct tcctgctgaa aaaagatcat cgcccgcggc
    12541 agatgtgatg cggaggtcac cagcaggaaa ggtgcgtcac caatcgcctg tttcactgct
    12601 gcagcttctt cttcggtatc ttttggcaaa tccagggtga taatttgctc gcgcggcacg
    12661 cccagcgatt gcgcaactct ggcacctact tcggctgtgc tcaccgtatt ggttttagct
    12721 acgcctcccg taaagatcag ttttgatccc ggattttcgc gccataagcg aataccttca
    12781 ttcaggcgtg gcagactgtt attgattaaa ttagagctcg gcgcccactg cgggttccag
    12841 gtataaccac ctccgagcac cacgatatag tccacttttt gcgaattatt ccatgtcgga
    12901 taagtgcttt cgatgggacg caacaggcgg tcggctatcg gttgcagact caataacaag
    12961 agcgccagcc acccaatgct aataaaaatc ttcccggttt tttgaaaacg gctaaaccac
    13021 agcagcgcca ggccagcgcc aataatcaac aacatcagcg gaagtggcag caacatattg
    13081 ccaatcactt ttttcagtgt aaaaagcatc ctttctggtt ccttttttga ccatatagca
    13141 agggatcgcc agcgatatta caccagacgg gttcattctc gccgtggctg tgacaaaata
    13201 gcgtctttat cagaaaacgt cttagccaac cggtggagaa agcaatgcag gatcgcaatt
    13261 ttgatgatat tgcggaaaag ttttcccgta acatttacgg caccaccaaa gggcagcttc
    13321 gacaggctat tctgtggcag gatctcgatc gcgtgctggc ggaaatgggc ccgcaaaaac
    13381 tgcgtgtgct ggatgctggc ggcggagaag ggcagaccgc aatcaaaatg gccgagcgcg
    13441 ggcatcaggt cattttatgc gatctttctg cgcagatgat cgaccgcgca aaacaggcgg
    13501 cagaagcaaa aggtgtgagc gacaacatgc aatttataca ttgcgccgct caggatgttg
    13561 cttcgcattt ggaaacgccc gttgatctga tattgttcca tgcggtgctc gagtgggtgg
    13621 ccgatccccg cagcgtatta cagaccctgt ggtcagtgtt gcgtccaggc ggcgtgttgt
    13681 cgttaatgtt ctacaatgcg catggtttgt tgatgcataa catggtcgcc gggaattttg
    13741 attacgtgca ggcgggaatg ccgaaaaaga aaaaacggac gctttcgcca gattatccac
    13801 gcgacccggc gcaggtttat ctgtggctgg aagaagctgg ttggcaaatt atgggtaaga
    13861 cgggcgttcg cgtgtttcat gattatctgc gcgagaagca ccagcagcgc gactgctatg
    13921 aagcattact tgaattagaa acgcgttatt gccgtcagga accgtatatt accctggggc
    13981 gttatattca tgtcaccgcg cgcaaaccgc agagcaagga taaagtatga gtgaattttc
    14041 ccagacagtc cccgaactgg ttgcctgggc cagaaaaaat gacttctcca tctcgctgcc
    14101 ggtagaccga ctctcttttc tgctggcggt tgccacgctg aacggcgagc gtctggatgg
    14161 tgagatgagt gaaggcgagc tggtggatgc attccgccat gtgagtgatg cgtttgagca
    14221 aaccagcgaa accatcggcg tgcgcgccaa caacgcgatc aacgacatgg tgcgtcaacg
    14281 tctgctgaac cgctttacca gcgagcaggc ggaagggaac gcaatttacc gtttgacgcc
    14341 gctcggcatc ggcatcactg actattacat tcgtcaacgc gagttttcta cgctgcgcct
    14401 ttctatgcaa ttgtcgattg tggcgggtga gctcaagcgc gcagcagatg ccgccgaaga
    14461 gggcggtgat gaatttcact ggcaccgtaa tgtttatgcg ccactgaaat attcggtagc
    14521 agaaattttc gacagtatcg acctgacgca acgtctgatg gatgaacagc agcagcaggt
    14581 gaaggatgat atcgcccagt tgttgaacaa agactggcgt gcggcgattt ccagctgtga
    14641 attgttgctt tcagaaactt ccggaacgct gcgtgaattg caggatacgc tggaagcggc
    14701 aggcgataaa ttgcaggcca acctgttacg cattcaggat gcgacgatga cccatgacga
    14761 cctgcatttt gtcgatcgtc tggtgttcga tcttcagagc aaactcgacc gtattatcag
    14821 ttggggccag caatccatcg acttgtggat tggctacgac cgccacgtac acaaatttat
    14881 tcgtactgcg atcgatatgg ataaaaaccg cgtctttgct cagcggttac gtcagtcggt
    14941 acaaacctat tttgatgagc cgtgggcgct aacctatgcc aatgccgatc gtctgctgga
    15001 tatgcgtgac gaagagatgg cactgcgcga tgaagaagtg actggggaac ttcctgagga
    15061 tctggagtac gaagagttta acgagatccg cgaacagctg gcggcgatca tcgaagaaca
    15121 acttgccgtg tacaaaacca gacaagtgcc gctggatctt ggtctggtgg tacgcgaata
    15181 tctgtcacag tatccgcgtg cacgtcactt tgacgttgcg cgcattgtta ttgatcaggc
    15241 ggtacgtctt ggcgtagcgc aagcagattt caccggactg ccagcgaaat ggcagccgat
    15301 taatgattac ggagccaagg tacaggcgca tgtcatcgac aaatattgaa caagtgatgc
    15361 cggttaagct ggcgcaggcg ctggcgaatc cgttatttcc ggcgctggat agcgccttac
    15421 gttcaggccg tcatattggc ctcgacgaac tggataatca tgcattcctg atggattttc
    15481 aggaatatct ggaagagttt tacgcgcgtt ataacgttga gcttattcgt gcaccggaag
    15541 ggttcttcta tctacgccca cgttccacca cgctgatccc tcgttccgtc ttgtcggaac
    15601 tggatatgat ggtcgggaaa atcctctgtt atctctatct cagcccggaa cggctggcga
    15661 atgaggggat ttttactcaa caggaactgt acgacgaact gctcaccctg gccgatgaag
    15721 cgaaactgct gaaactggtg aacaaccgtt caaccggttc agacgttgac cgtcagaagt
    15781 tgcaggaaaa agtacgttct tcgctcaacc gtctgcgtcg tttaggcatg gtgtggttta
    15841 tgggccacga cagcagcaag ttccgcatta ccgaatcggt gttccgcttc ggagccgatg
    15901 tgcgtgctgg cgacgatccc cgtgaagcac agcgtcgcct gattcgtgat ggtgaagcaa
    15961 tgccgattga aaaccatctg caactcaacg atgaaaccga agagagtcag ccagatagcg
    16021 gagaggaaga ataatgattg aacgcggtaa atttcgctca ctgacgctga ttaactggaa
    16081 cggctttttt gcccgaactt ttgaccttga cgagctggtc acgacgcttt ctggcggtaa
    16141 cggggcgggt aaatccacca ccatggcggc gttcgttacg gcgcttatcc ccgatctgac
    16201 cctgctgcat ttccgtaaca ctacggaagc cggggccacc agcggttcgc gcgataaagg
    16261 tctgcacggt aagctgaaag cgggtgtctg ttattcgatg cttgacacca ttaactcgcg
    16321 ccaccagcgt gtagtggtcg gtgtgcgtct gcaacaagtt gccggacgcg atcgtaaagt
    16381 ggatatcaaa ccatttgcca ttcagggcct gccgatgtcg gtgcagccga cacaactggt
    16441 gaccgaaact ctgaacgaac gccaggcgcg cgtgctgccg cttaacgagc tgaaagacaa
    16501 gctcgaggcg atggaaggcg tgcagtttaa acagttcaac tccattaccg attaccactc
    16561 gctgatgttc gatctgggca tcatcgcgcg tcgtctgcgc tcagcgtctg accgtagtaa
    16621 attctatcgt ctgatcgaag cctcgctgta tggcgggatc tccagcgcca ttactcgttc
    16681 tctgcgcgac tacctgttgc cggaaaacag tggggtgcgg aaagcgttcc aggacatgga
    16741 agcggcgctg cgtgaaaacc gcatgacgct ggaagcgatt cgtgtcaccc agtcggaccg
    16801 tgacctgttt aagcatctga tcagcgaagc caccaactac gtggcggcgg actacatgcg
    16861 tcacgccaac gagcgccgtg ttcatctcga taaggccctg gagtttcgtc gcgagctaca
    16921 tacttcgcgt cagcaactgg cggctgagca gtacaaacac gtcgatatgg cgcgagagct
    16981 ggctgagcac aacggtgccg aaggcgatct ggaagcggat tatcaggcgg ccagcgatca
    17041 cctgaacctg gtgcaaaccg cactgcgtca gcaggagaag atcgaacgct acgaagcgga
    17101 tctcgatgag ctgcagatcc gtctggaaga gcaaaacgaa gtggtggcag aagccatcga
    17161 acgccaggaa gagaacgagg ctcgtgcgga agctgccgaa ctggaagtgg acgagctgaa
    17221 aagccagctt gctgactacc agcaggcgct ggacgtccag caaacgcgcg cgatccagta
    17281 taaccaggcg attgctgcgc ttaatcgtgc caaagaactg tgctatctgc cggacttaac
    17341 cgccgacagc gctgccgaat ggctggaaac cttccaggcg aaagagctgg aagcgaccga
    17401 aaaaatgctc tctcttgagc agaaaatgag catggcgcaa accgcgcaca gccagtttga
    17461 gcaggcttat cagctggtgg tggcaatcaa cggcccgctg gcgcgtaacg aggcgtggga
    17521 tgtcgctcgc gaactattgc gcgaaggggt cgatcagcgt cacctggcag agcaggttca
    17581 gccgttgcgg atgcgcttaa gcgaactgga acagcgtctg cgtgagcagc aagaagctga
    17641 gcgtctgctg gcagatttct gcaaacgtca gggcaagaat tttgatattg atgaactgga
    17701 agctctgcat caggagctgg aagcacgcat tgcctctctt tccgatagcg tctctaacgc
    17761 ccgagaagag cggatggcac tgcgccagga gcaggaacag ctgcagtctc gcattcagag
    17821 tttgatgcag cgtgcgccgg tttggctggc agcgcaaaac agtctcaacc agttgagcga
    17881 acagtgcggc gaagagttca cctccagcca ggatgtcacc gaatttctgc aacagttgct
    17941 ggagcgtgag cgcgaggcaa ttgttgaacg cgatgaagta ggcgcgcgca aaaacgccgt
    18001 cgatgaagag atcgaacgtt taagccagcc aggtggttct gaagatcagc gtctgaacgc
    18061 gctggcggag cgttttggtg gtgtgctgct gtcagaaatt tatgacgacg ttagcctgga
    18121 agatgcgccg tacttctcag cgctgtatgg cccgtcacgc cacgcaatcg tggtgccaga
    18181 tctgtcacag gtaaccgaac acctggaagg cttgaccgat tgcccggaag atctctattt
    18241 gatcgaaggg gatccgcagt cattcgatga cagcgtgttc agcgttgatg agctggaaaa
    18301 agcggtggtg gtgaaaatcg ccgatcgtca gtggcgttat tcacgtttcc cggaagtgcc
    18361 gctgtttggt cgtgctgcgc gtgaaagccg tattgaaagc ctccatgccg agcgggaagt
    18421 gctttccgaa cgcttcgcca cgctctcctt tgatgtacag aaaacccagc gtctgcatca
    18481 ggcgttcagc cgctttatcg gcagtcatct ggcggtggcg tttgagtctg acccggaagc
    18541 agaaatccgt caactgaaca gccgtcgcgt cgaactggag cgggcgttga gtaatcatga
    18601 aaatgataac cagcagcagc gtattcagtt tgagcaggcg aaagagggcg ttacggcgct
    18661 gaaccgcatt ctgccgcgtc tcaacctgtt ggctgatgac agcctggcgg atcgcgtcga
    18721 tgaaatccgc gaacgtctgg atgaagccca ggaagccgcg cgttttgttc agcagtttgg
    18781 caatcaactg gcgaaactgg aaccgatcgt ttcggtattg cagagcgacc cggaacagtt
    18841 cgaacagtta aaagaagatt acgcgtactc tcagcagatg cagcgcgatg cccgtcagca
    18901 ggcgtttgcc ctgacggaag tggtacagcg tcgtgcgcac tttagctact ctgactcggc
    18961 agaaatgctt agcggtaaca gcgatctcaa cgaaaaactg cgtgaacgtc tggaacaggc
    19021 ggaagcggag cgtacccgcg ctcgtgaagc gttgcgcgga cacgcagcgc agttgaatca
    19081 gtacaaccag gtgctggctt cgctgaaaag ttcgtatgac accaaaaaag agctacttaa
    19141 cgatctgcaa cgtgaattgc aggatatcgg cgtgcgtgct gatagcgggg cagaagagcg
    19201 ggcacgtatt cgccgtgacg agttgcacgc gcaactgagc aataaccgtt cacgacgcaa
    19261 tcaactggaa aaagcgctta cattctgcga agcggagatg gacaacctga cccgtaaact
    19321 gcgcaagctg gagcgggatt actttgagat gcgcgagcag gtagtgaccg ccaaagcggg
    19381 ctggtgtgcg gtgatgcgca tggtgaaaga taatggcgtt gagcgtcgct tacaccgtcg
    19441 tgagctggct tatctctccg ctgatgattt gcgttccatg tcggataagg cgttaggtgc
    19501 gctgcgtctg gcggtggcag ataacgaaca tctgcgcgac gtgctgcgga tgtcggaaga
    19561 tccaaaacgt ccggaacgta aaattcagtt cttcgtggcg gtttatcagc atctgcgtga
    19621 acgtattcgt caggatatta ttcgtaccga tgatccggtg gaagctatcg aacagatgga
    19681 gatcgagctt agccgtctga ccgaagaatt aacctcccgt gaacagaaac tggcgatcag
    19741 ttcccgcagc gtggcgaaca tcattcgcaa aaccattcag cgcgagcaga accgtatccg
    19801 tatgctcaac caggggttgc agaacgtatc gtttggccag gtgaacagcg tacgtctgaa
    19861 cgtgaacgta cgtgaaacgc acgccatgct gctggatgtg ctctctgaac agcacgagca
    19921 gcatcaggat ctgtttaaca gcaaccgcct gaccttctca gaagcgctgg cgaaactgta
    19981 tcaacgtctt aacccgcaga ttgatatggg gcagcgcacg ccgcagacca tcggtgaaga
    20041 actgctggat taccgcaact atctggaaat ggaagttgag gttaaccgtg gttccgatgg
    20101 ctggttgcgt gcagagtctg gtgcattgtc gaccggtgag gcgattggta ccggtatgtc
    20161 gattctggtg atggtggtac aaagctggga agatgaatct cgccgcctgc gcggtaaaga
    20221 tatctctcct tgccgcctgc tgttcctcga tgaagcagcg cgactggacg cccgttccat
    20281 cgccacgctg tttgaattgt gtgaacgttt gcaaatgcag ctcattatcg cagcgccgga
    20341 aaatatcagc ccggagaaag gcaccaccta taaactggtg cgtaaagtct tccagaatac
    20401 cgaacacgtt cacgtcgtcg gcctgcgagg atttgcgcct caactccctg aaacgcttcc
    20461 aggaagtgac gaagcgcctt ctcaggcgag ttaaagaaaa ggcggcagca atgccgcctt
    20521 ttctttttcc gaaaactccg tttctgcact aaaaaagtgg cacattacgg cgcgcttttc
    20581 tttaaacttc tttacattag gttatgtaaa aacgtatcgg cgtttatata ctgaagataa
    20641 gtctgatgag taacaggctt gctcgtcata ctttcgtgag tattggcgtt gtacaggcaa
    20701 gtcgtaaaat aacagcctgg ctattcagag tatgataaaa acagggggca agggatgttg
    20761 cttaatatga tgtgtggtcg tcggctgtcg gcaatcagtt tgtgcctggc cgtaacattc
    20821 gctccactgt tcaatgcgca ggccgatgag cctgaagtaa tccctggcga cagcccggtg
    20881 gctgtcagtg aacagggcga ggcactgccg caggcgcaag ccacggcaat aatggcgggg
    20941 atccagccat tgcctgaagg tgcggctgaa aaagcccgca cgcaaatcga atctcaatta
    21001 cccgcaggtt ataagccggt ttatcttaac cagcttcaat tgttgtatgc cgcacgcgat
    21061 atgcaaccca tgtgggaaaa ccgtgatgct gttaaagcct tccagcaaca gctggcagag
    21121 gtggcgattg ccggtttcca gccgcagttt aataaatggg tagagttact gaccgatcct
    21181 ggtgttaacg ggatgacacg cgacgtggtg ctctctgatg cgatgatggg ctatctccat
    21241 ttcattgcaa atattccggt caaaggcact cgctggctat atagcagtaa accttatgcg
    21301 cttgcaacgc cgccgctttc ggtgattaac caatggcagc tggcgctgga taaaggtcaa
    21361 ttgccgacgt ttgttgcagg actggcactg cagcatccgc aatatgcggc gatgcatgaa
    21421 tcgttactgg ccttactcag tgacaccaaa ccgtggcccc aactgaccgg caaagcaacg
    21481 ttgcgcccag gacagtggag taacgacgta ccggcgttgc gcgaaatatt gcaacgtaca
    21541 ggcatgttgg acgggggacc gaaaattacg ctacctggcg atgacacgcc aactgacgcg
    21601 gtagtcagcc catccgctgt tactattgaa acagcagaaa ctaagccgat ggacaagcaa
    21661 actacgtctc gtagtaaacc tgcgcctgtc gttcgtgccg cctacgataa tgaactggtg
    21721 gaagccgtta aacgttttca gacatggcaa ggattgggag cagatggcgc tattggcccg
    21781 gcaacgcgtg actggttaaa cgtaacgccc gcccagcgtg ctggcgtgtt ggcactcaac
    21841 atccagcgat tgcgcttgct gccaacagag ctttctaccg ggatcatggt taacattcca
    21901 gcctattcgc tggtctacta tcagaacggc aatcaggtgc tggattcgcg agtcattgtc
    21961 ggtcgccccg atcgcaaaac gccgatgatg agcagtgcac ttaacaacgt agtggtaaac
    22021 ccgccgtgga acgtaccacc aactctggca cgcaaagata ttctgccgaa agtgcgcaac
    22081 gatccgggat atctcgaaag ccatggctat acggtgatgc gcggctggaa cagcagagaa
    22141 gcgattgacc catggcaggt tgactggtct acaatcacgg cctcgaattt accgttccgc
    22201 ttccagcagg ctccaggccc acggaactcg ctggggcgct ataaattcaa tatgccgagt
    22261 tcagaggcca tttatttgca tgacacgccg aaccacaatc tgttcaagcg tgatacacgc
    22321 gcattgagct caggctgtgt acgagtgaat aaagcttccg atctggcgaa tatgctgttg
    22381 caggatgcag gctggaatga caaacgtatt tctgatgcgc tgaagcaggg cgatacgcgt
    22441 tacgtcaata ttcggcagtc gattccggtg aatctctact atctgacggc ctttgttggt
    22501 gcagatggtc gtacccagta tcgtacagat atttacaatt atgatctgcc agcgcgatcc
    22561 agctcgcaaa tcgtatcgaa agcggaacaa ttaatcaggt aaatgaagca gttcggggaa
    22621 atcagttgtc gtaataatta gcatgatcgg gggcgattct ctgcagcccc cgtcactgct
    22681 ggggttgagt aatcttgacg tctgctttac gggcggttaa ggtgcctctt gtgcgccaga
    22741 agtgcatata aacgataaca ttgacctgta gacttgatta tcatggacaa attcgacgct
    22801 aatcgccgca aactgctggc gcttggtggc gttgcactcg gtgccgccat cctgccgacc
    22861 ccggcgtttg caacactctc taccccacgc ccgcgcattt tgacactcaa taatcttcat
    22921 accggagagt caatcaaagc ggagtttttc gatggcagag gctatattca ggaagaattg
    22981 gcaaaactta accatttttt ccgcgattac cgcgcgaaca aaataaagtc catcgaccca
    23041 ggattattcg accagttgta tcgcctgcaa gggttgttag gcacccgcaa accggtgcaa
    23101 ctcatttccg gttatcgttc cattgatacc aacaatgaac tacgcgcccg cagccgtgga
    23161 gtagcgaaga aaagctatca cactaaaggc caggcgatgg atttccacat tgaaggtatc
    23221 gcgttaagca atattcgcaa agccgcgtta tctatgcgcg caggtggtgt aggatattac
    23281 ccacgtagta actttgtgca tattgatacc gggccagcac ggcactggta gtaatcgctt
    23341 aacgaaacag gggcagtatg aactatcgta ttattccggt caccgcattc tcccagaact
    23401 gttcattgat ctggtgtgaa caaacccgtc tggccgcact ggtcgatcct ggcggcgatg
    23461 cggaaaaaat caaacaggaa gttgatgcca gcggcctgac actgatgcag atcctgctga
    23521 ctcatggtca tctggaccac gttggcgcag cggcggaact ggcgcaacat tacggcgtgc
    23581 cggtttttgg cccggaaaaa gaagatgagt tctggctgca aggcttgcct gcgcaaagtc
    23641 gtatgtttgg tctggaagag tgccagccgc tgacgccaga tcgttggctg aacgaaggcg
    23701 ataccatcag catagggaat gtgactttac aggtgttaca ttgccctggg catacgccgg
    23761 gtcatgtcgt gttttttgat gatcgggcaa agctgctgat ttctggcgac gttattttca
    23821 aaggtggagt agggcgcagt gacttcccgc gtggcgatca taatcaactg atttcttcaa
    23881 tcaaagataa attgctgcca ctgggggatg acgtgacatt tattccgggt cacggaccat
    23941 tatccacact tggttatgag cgcctgcata atcccttcct gcaagacgaa atgcccgtct
    24001 ggtaaaggtg cataaaaaag cccgctttca agcgggcttc attgttttta acgcttacag
    24061 cactgccaca atcgcttcgc acagcggcgc catgttatct ggtgtcatcc cggccacgtt
    24121 tacgcgacca gaagcaacag catatacgcc aaactcttcg cgcagacgca gcacttgttc
    24181 ttttgtcagg ccactgaagg agaacatgcc gttctgtttg atgataaagc tgaagtcgcg
    24241 atttgcgcct ttttcctgca gcgtattgac gaacaactga cgcatacgct gaatacgctg
    24301 gcgcatatca gtcagctctt gttcccaaat cgcacgtaat gcatcgttgc tcaggatggt
    24361 ggcaacaaca gaagcgccgt gtgctggtgg gttagagtag ttagcgcgaa tcgccgcttt
    24421 catttggctg aatgcgcgat caacggtttc actgtcagca gcaaccagag tacaagcgcc
    24481 aacacgctcg ttgtacaggc caaagttttt agagtaggaa ctggcaacaa tcagctcttt
    24541 atgcatagca gcgaaagcgc gcagtccttc agcatcttct tccagaccac gggcaaaacc
    24601 ctggtaagcg aagtcaaaca gcggtaacca gcctttctca acggagagtt gtgccagtgt
    24661 ctgccattgt tccagcgtag ggtcgatacc ggttgggtta tggcagcagc catggaacag
    24721 cactacatcg ccagcctgag cttcgtttag actgttaatc agtgcatcga agtcaagggt
    24781 gtgattttcc gcatcataat aagcgtattc acgaacttcc agacctgcag agttaaagac
    24841 gctcttatgg ttcggccagc ttgggttgct cacccacaca cgcttaacgc tggtattttt
    24901 tgccaggaaa tcggcagcca cgcgtagtgc gccagtgcca cccggagtct gtgccgtgcg
    24961 agcacgtttg tcattgatca gggcgctacc tttaccaaac agtagttcct gagtgcagcg
    25021 accaaattca gggatgccgt caatgccgag gtaatttttg gtggtttcat tttcgagcag
    25081 atactgttca gcctttttca cactggtcag taccggggtt ttgcccgtct catctttata
    25141 gacaccaatc ccgaggttaa ttttgccggg acgttcatcg gcacgaaaca gatcggccag
    25201 gcccagaatc gggtcggcag gagcggcggt aatgttctca aacatgacga ggttccatta
    25261 tggttacaga agggaagtcc gctatcaggg taacgggaga tttacaaaat tccaactatt
    25321 actgatgaaa acgcaggctg tttttgcaag acgtgagatt gctctggaag gcataaaaaa
    25381 aacaggacca aagtcctgtt ttttcggcat ttaacaaaga ggtgtgctat tagaactggt
    25441 aaacgatacc cacagcaacg gtgtcgtcag aacctacgcc cagtttgttg tcagaatcga
    25501 tctggttgat gatgtagtca acataggtgg acatgttttt gttgaagtag taggttgcgc
    25561 ccacttcaaa gtagttcacc agatcaacat caccgatacc ttctacgtct ttcgctttag
    25621 atttggtgta agcgatggac ggacgcagac cgaaatcgaa ctggtattgc gcaactaaca
    25681 gaacgtcttg cgttttgttg gcgaagccgc tgatgtttgt aaatttatta gtgatcggcg
    25741 tagcgttacg ggtttcaccg tagttcgctg ccaggtagat gttgttcgcg tcatacttca
    25801 gaccagtagc ccactgttca gcttttttac cgttgccaag aggttgagct tcttgcaggt
    25861 tggtacggtc agcggcgcca taagcaccaa cgataccaaa gccttcgtat tcgtagctga
    25921 tagaaccgcc aacaccgtcg ccgttagagc ggcgtgcagt gtcacgctcg tttttaccca
    25981 ggtactgaac agcgaagttc aggccatcaa ccagaccaaa gaagttggag ttacgatagg
    26041 tagcaacgcc gccaacacga ccaacgaaga agtcatcgct gtatgcagta tcaccaccaa
    26101 attctggcag catatcggtg taacccagtg catcataaac cacaccgtag ttacggccgt
    26161 aatcgaaaga accaacgtca gcgtatttaa gacccgcgaa tgccagacgc gttttgttac
    26221 cagtttgagc gtcagcgcct tcagagttgt taccctggaa gttatattcc cactgaccat
    26281 aaccggtcag atcggaattg atttgagttt cccctttaaa accaagacgg gcataggtca
    26341 tgtcaccatt gccaccgtaa ctgttttcac cgttaccctt ggagaaataa tgcagaccga
    26401 cagctttacc gtacagatct actttgttgc catctttgtt atagatttct gcagcgtttg
    26461 cagtacctgc tactaacaga gcagggacga tcactgccag aatattgcgc ttcatcatta
    26521 tttattaccc tcatggtttt tttatgacac ctgccactgc cgtcaataag ttctgtcaat
    26581 aaaaatttac ggaactattg atgagagttt ggtgtcttta tgtgtctgca ggcatctttc
    26641 cattcaaact aacgtttcgc taccgtgaaa gtgctacaaa gataaagatt tggtttcaaa
    26701 aagaaaaaat atgtaaccaa
//
LOCUS       DADSYM010000061        26672 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2415
            SAMN08039579-rid11723173.denovo.061, whole genome shotgun sequence.
ACCESSION   DADSYM010000061 DADSYM010000000
VERSION     DADSYM010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039579
            Sequence Read Archive: SRR6362533
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 26672)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26672)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980162.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 03:39:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,484
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 4,347
            CDSs (with protein)               :: 4,347
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 43 of 85
            Pseudo Genes (incomplete)         :: 37 of 85
            Pseudo Genes (internal stop)      :: 19 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26672
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039579-rid11723173.denovo.061"
                     /strain="LGE2415"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8302 N 95.9628 W"
                     /collection_date="2015-08-24"
     gene            37..768
                     /gene="artJ"
                     /locus_tag="J1Y83_002120"
     CDS             37..768
                     /gene="artJ"
                     /locus_tag="J1Y83_002120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter substrate-binding
                     protein"
                     /protein_id="HBA7630267.1"
                     /translation="MKKLVLAALLASFTFGASAAEKINFGVSATYPPFESIGANNEIV
                     GFDIDLAKALCKQMQAECTFTNHAFDSLIPSLKFRKYDAVISGMDITPERSKQVSFTT
                     PYYENSAVVIAKKDTYKTFADLKGKRIGMENGTTHQKYIQDQHPEVKTVSYDSYQNAF
                     IDLKNGRIDGVFGDTAVVNEWLKTNPQLGVATEKVTDPQYFGTGLGIAVRPDNKALLE
                     KLNNALAAIKADGTYQKINDQWFPQ"
     gene            complement(797..1924)
                     /gene="rlmC"
                     /locus_tag="J1Y83_002121"
     CDS             complement(797..1924)
                     /gene="rlmC"
                     /locus_tag="J1Y83_002121"
                     /EC_number="2.1.1.189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(747)-C(5))-methyltransferase
                     RlmC"
                     /protein_id="HBA7630268.1"
                     /translation="MQCALYDAGRCRSCQWITQPIPEQLSAKTADLKNLLADFPVEEW
                     CAPVSGPEQGFRNKAKMVVSGSVEKPLLGMLHRDGTPEDLCDCPLYPASFAPVFAALK
                     PFIARAGLTPYNVARKRGELKYILLTESQSDGGMMLRFVLRSDTKLTQLRKALPWLQE
                     QLPQLKVITVNIQPVHMAIMEGETEIYLTEQQALAERFNDVPLWIRPQSFFQTNPAVA
                     SQLYATARDWVRQLPVKHMWDLFCGVGGFGLHCATPDMQLTGIEIAPEAIACAKQSAA
                     ELGLTRLQFQALDSTQFATAQGEVPELVLVNPPRRGIGKPLCDYLSTMAPRFIIYSSC
                     NAQTMAKDIRELPGYRIERVQLFDMFPHTAHYEVLTLLVKQ"
     gene            complement(1965..2453)
                     /locus_tag="J1Y83_002122"
     CDS             complement(1965..2453)
                     /locus_tag="J1Y83_002122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjO family protein"
                     /protein_id="HBA7630269.1"
                     /translation="MEDETLGFFKKTSSSHARLNVPALVQVAALAIIMIRGLDVLMIF
                     NTLGVRGIGEFIHRSVQTWSLTLVFLSSLVLVFIEIWCAFSLVKGRRWARWLYLLTQI
                     TAASYLWAASLGYGYPELFSIPGESKREIFHSLMLQKLPDMLILMLLFVPSTSRRFFQ
                     LQ"
     gene            complement(2513..3358)
                     /gene="potI"
                     /locus_tag="J1Y83_002123"
     CDS             complement(2513..3358)
                     /gene="potI"
                     /locus_tag="J1Y83_002123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter permease PotI"
                     /protein_id="HBA7630270.1"
                     /translation="MNNLPVVRSPWRIVILLLGFTFLYAPMLMLVIYSFNSSKLVTVW
                     AGWSTRWYGELLRDDAMMSAVGLSLTIAACAATAAAILGTIAAVVLVRFGRFRGSNGF
                     AFMITAPLVMPDVITGLSLLLLFVALAHAIGWPADRGMLTIWLAHVTFCTAYVAVVIS
                     SRLRELDCSIEEAAMDLGATPLKVFFVITLPMIMPAIISGWLLAFTLSLDDLVIASFV
                     SGPGATTLPMLVFSSVRMGVNPEINALATLILGAVGIVGFIAWYLMARAEKQRIRDIQ
                     RARRG"
     gene            complement(3355..4299)
                     /gene="potH"
                     /locus_tag="J1Y83_002124"
     CDS             complement(3355..4299)
                     /gene="potH"
                     /locus_tag="J1Y83_002124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter permease PotH"
                     /protein_id="HBA7630271.1"
                     /translation="MSTLEPAAQPKPFKLWLSQLQMKHGRKLVIALPYIWLILLFLLP
                     FLIVFKISLAEMVRAIPPYTELMEWADGQLSITLNLGNFLQLTDDPLYFDAYLQSLQV
                     AAISTICCLLIGYPLAWAVAHSKPSTRNILLLLVILPSWTSFLIRVYAWMGILKSNGV
                     LNNFLLWLGVIDQPLTILHTNFAVYIGIVYAYVPFMVLPIYTALIRIDYSLVEAALDL
                     GARPLKTFFTVIVPLTKGGIIAGSMLVFIPAVGEFVIPELLGGPDSIMIGRVLWQEFF
                     NNRDWPVASAVAIIMLLLLIVPIMWFHKHQQKSVGEHG"
     gene            complement(4309..5442)
                     /gene="potG"
                     /locus_tag="J1Y83_002125"
     CDS             complement(4309..5442)
                     /gene="potG"
                     /locus_tag="J1Y83_002125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000996010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter ATP-binding subunit
                     PotG"
                     /protein_id="HBA7630272.1"
                     /translation="MNDAIPRPQAKTRKALTPLLEIRNLTKSYDGQHAVDDVSLTIYK
                     GEIFALLGASGCGKSTLLRMLAGFEQPSAGQIMLDGVDLSQVPPYLRPINMMFQSYAL
                     FPHMTVEQNIAFGLKQDKLPKAEIASRVNEMLGLVHMQEFAKRKPHQLSGGQRQRVAL
                     ARSLAKRPKLLLLDEPMGALDKKLRDRMQLEVVDILERVGVTCVMVTHDQEEAMTMAG
                     RIAIMNRGKFVQIGEPEEIYEHPTTRYSAEFIGSVNVFEGVLKERQEDGLVLYSPGLV
                     HPLKVDVDASVVDNVPVHVALRPEKIMLCEEPPANGCNFAVGEVIHIAYLGDLSVYHV
                     RLKSGQMISAQLQNAHRHRKGLPTWGDEVRLCWEVDSCVVLTV"
     gene            complement(5537..6649)
                     /gene="potF"
                     /locus_tag="J1Y83_002126"
     CDS             complement(5537..6649)
                     /gene="potF"
                     /locus_tag="J1Y83_002126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein PotF"
                     /protein_id="HBA7630273.1"
                     /translation="MTALNKKWLSGLVAGALMAVSVSTLAAEQKTLHIYNWSDYIAPD
                     TVANFEKETGIKVVYDVFDSNEVLEGKLMAGSTGFDLVVPSASFLERQLTAGVFQPLD
                     KSKLPEWKNLDPELLKLVAKHDPDNKFAMPYMWATTGIGYNVDKVKAVLGENAPVDSW
                     DLILKPENLEKLKSCGVSFLDAPEEVFATVLNYLGKDPNSTKADDYTGPATDLLLKLR
                     PNIRYFHSSQYINDLANGDICVAIGWAGDVWQASNRAKEAKNGVNVSFSIPKEGAMAF
                     FDVFAMPADAKNKDEAYQFLNYLLRPDVVAHISDHVFYANANKEATPLVSAEVRDNPG
                     IYPPADVRAKLFTLKVQDPKIDRVRTRAWTKVKSGK"
     gene            complement(6999..7475)
                     /locus_tag="J1Y83_002127"
     CDS             complement(6999..7475)
                     /locus_tag="J1Y83_002127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjN domain-containing protein"
                     /protein_id="HBA7630274.1"
                     /translation="MTSLVVPGLDTLRQWLDDLGMSFFECDNCQALHLPHMQNFDGVF
                     DAKIDLIDNTILFSAMAEVRPSAVLPLAADLSAINASSLTVKAFLDMQDDNLPKLVVC
                     QSLSVMQGVTYEQFAWFVRQSEEQISMVILEANAHQLLLPTDDEGQNNVTENYFLH"
     gene            complement(7563..8465)
                     /gene="rimK"
                     /locus_tag="J1Y83_002128"
     CDS             complement(7563..8465)
                     /gene="rimK"
                     /locus_tag="J1Y83_002128"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6--L-glutamate ligase"
                     /protein_id="HBA7630275.1"
                     /translation="MKIAILSRDGTLYSCKRLREAAIQRGHLVEILDPLSCYMNINPA
                     ASSIHYKGRKLPHFDAVIPRIGTAITFYGTAALRQFEMLGSYPLNESVAIARARDKLR
                     SMQLLARQGIDLPVTGIAHSPDDTSDLIDMVGGAPLVVKLVEGTQGIGVVLAETRQAA
                     ESVIDAFRGLNAHILVQEYIKEAQGCDIRCLVVGDEVVAAIERRAKEGDFRSNLHRGG
                     AASVASITPQEREIAIKAARTMALDVAGVDILRANRGPLVMEVNASPGLEGIEKTTGI
                     DIASKMIRWIERYATTEYCLKTGG"
     gene            complement(8526..9248)
                     /gene="nfsA"
                     /locus_tag="J1Y83_002129"
     CDS             complement(8526..9248)
                     /gene="nfsA"
                     /locus_tag="J1Y83_002129"
                     /EC_number="1.5.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase NfsA"
                     /protein_id="HBA7630276.1"
                     /translation="MTPTIELICGHRSIRHFTDEPISEAQREAIINSARATSSSSFLQ
                     CSSIIRITDKALREELVTLTGGQKHVAQAAEFWVFCADFNRYLQICPDAQLGLAEQLL
                     LGVVDTAMMAQNALTAAESLGLGGVYIGGLRNNIEAVTELLKLPQHVLPLFGLCLGWP
                     ADNPDLKPRLPASILVHENSYQPLDKDALAQYDEQLAEYYLTRGSNNRRDTWSDHIRR
                     TIIKESRPFILDYLHKQGWATR"
     gene            complement(9232..9519)
                     /locus_tag="J1Y83_002130"
     CDS             complement(9232..9519)
                     /locus_tag="J1Y83_002130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1418 family protein"
                     /protein_id="HBA7630277.1"
                     /translation="MRAIGKLPKSVLILEFIGMMLLAVALLSVSDSLSLPEPFSRPEV
                     QILMIFLGVLLMLPAAVVVILQVAKRLAPQLMNHPPQYSRSEREKDNDANH"
     gene            9679..9936
                     /gene="grxA"
                     /locus_tag="J1Y83_002131"
     CDS             9679..9936
                     /gene="grxA"
                     /locus_tag="J1Y83_002131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 1"
                     /protein_id="HBA7630278.1"
                     /translation="MQTVIFGRPGCPYCVRAKDLAEKLSNERDDFQYQYVDIRAEGIT
                     KEDLQQKAGKPVETVPQIFVDQQHIGGYTDFAAWAKENLDA"
     gene            complement(9966..10343)
                     /locus_tag="J1Y83_002132"
     CDS             complement(9966..10343)
                     /locus_tag="J1Y83_002132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA7630279.1"
                     /translation="MKHKQRWAGAICCFVLFIVVCLFLATHMKGAFRAAGHPEIGLLF
                     FILPGAVASFFSHRREVLKPLFGAMLAAPCSMLIMRLFFSPTRSFWQELAWLLSAVFW
                     CALGALCFLFISSLFKPQHRKNQ"
     gene            10613..12298
                     /locus_tag="J1Y83_002133"
     CDS             10613..12298
                     /locus_tag="J1Y83_002133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001024888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate:alanine antiporter"
                     /protein_id="HBA7630280.1"
                     /translation="MNINVAELLNGNYILLLFVVLALGLCLGKLRLGSIQLGNSIGVL
                     VVSLLLGQQHFSINTDALNLGFMLFIFCVGVEAGPNFFSIFFRDGKNYLMLALVMVGS
                     ALVIALGLGKLFGWDIGLTAGMLAGSMTSTPVLVGAGDTLRHSGMESRQLSLALDNLS
                     LGYALTYLIGLVSLIVGARYLPKLQHQDLQTSAQQIARERGLDTDANRKVYLPVIRAY
                     RVGPELVAWTDGKNLRELGIYRQTGCYIERIRRNGILANPDGDAVLQMGDEIALVGYP
                     DAHARLDPSFRNGKEVFDRDLLDMRIVTEEVVVKNHNAVGKRLAQLKLTDHGCFLNRV
                     IRSQIEMPIDDNVVLNKGDVLQVSGDARRVKTIADRIGFISIHSQVTDLLAFCAFFVI
                     GLMIGMITFQFSTFSFGMGNAAGLLFAGIMLGFMRANHPTFGYIPQGALSMVKEFGLM
                     VFMAGVGLSAGSGINNGLGAIGGQMLIAGLIVSLVPVVICFLFGAYVLRMNRALLFGA
                     MMGARTCAPAMEIISDTARSNIPALGYAGTYAIANVLLTLAGTIIVMVWPGLG"
     gene            complement(12474..13010)
                     /locus_tag="J1Y83_002134"
     CDS             complement(12474..13010)
                     /locus_tag="J1Y83_002134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBA7630281.1"
                     /translation="MRRANDPQRREKIIQATLEAVKLYGIHAVTHRKIATLAGVPLGS
                     MTYYFSGIDELLLEAFSRFTEIMSRQYQAFFSDVRDAPGACQAITDMIYSSQVATPDN
                     MELMYQLYALASRKPLLKTVMQNWMQRSQQTLEQWFEPGTARTLDAFIEGMTLHFVTD
                     RKPLSREEILRMVERIAG"
     gene            13094..14302
                     /locus_tag="J1Y83_002135"
     CDS             13094..14302
                     /locus_tag="J1Y83_002135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBA7630282.1"
                     /translation="MTVNSSRNALKRRTWALFMFFFLPGLLMASWATRTPAIRDILSV
                     SIAEMGGVLFGLSIGSMSGILCSAWLVKRFGTRNVILVTMSCALIGMMILSLALWLTS
                     PLLFAVGLGVFGASFGSAEVAINVEGAAVEREMNKTVLPMMHGFYSLGTLAGAGVGMA
                     LTAFGVPATVHILLAALVGIAPIYIAIQAIPDGTGKNAADGTQHGEKGVPFYRDIQLL
                     LIGVVVLAMAFAEGSANDWLPLLMVDGHGFSPTSGSLIYAGFTLGMTVGRFTGGWFID
                     RYSRVAVVRASALMGALGIGLIIFVDSAWVAGVSVVLWGLGASLGFPLTISAASDTGP
                     DAPTRVSVVATTGYLAFLVGPPLLGYLGEHYGLRSAMLVVLALVILAAIVAKAVAKPD
                     TKTQTAMENS"
     gene            14302..15117
                     /gene="ybjI"
                     /locus_tag="J1Y83_002136"
     CDS             14302..15117
                     /gene="ybjI"
                     /locus_tag="J1Y83_002136"
                     /EC_number="3.1.3.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415365.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-amino-6-(5-phospho-D-ribitylamino)uracil
                     phosphatase"
                     /protein_id="HBA7630283.1"
                     /translation="MSIKLIAVDMDGTFLSDQKTYNRERFMAQYQQMKAQGIRFVVAS
                     GNQYYQLISFFPEIANEIAFVAENGGWVVSEGKDVFNGELSKDAFTTVVEHLLTRPEV
                     EIIACGKNSAYTLKKYDDAMKTVAEMYYHRLEYVDNFDNLEDIFFKFGLNLSDELIPQ
                     VQKALHEAIGDIMVPVHTGNGSIDLIIPGVHKANGLRQLQKLWGIDDSEVVVFGDGGN
                     DIEMLRQAGFSFAMENAGSAVVAAAKYRAGSNNREGVLDVIDKVLKHEAPFNQ"
     gene            15202..15486
                     /locus_tag="J1Y83_002137"
     CDS             15202..15486
                     /locus_tag="J1Y83_002137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA7630284.1"
                     /translation="MIMKNCLLLDALLMGFTGVAMAQSVTVDVPSGYKVVVVPDSVSV
                     PQAVSVATVPQTVYVAPAPAPAYRPHPYVRHLASVGEGMVIEHQIDDHHH"
     gene            complement(15527..16759)
                     /gene="mdfA"
                     /locus_tag="J1Y83_002138"
     CDS             complement(15527..16759)
                     /gene="mdfA"
                     /locus_tag="J1Y83_002138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdfA"
                     /protein_id="HBA7630285.1"
                     /translation="MQNKLASGARLGRQALLFPLCLVLYEFSTYIGNDMIQPGMLAVV
                     EQYQAGIDWVPTSMTAYLAGGMFLQWLLGPLSDRIGRRPVMLAGVVWFIVTCLAILLA
                     QNIEQFTLLRFLQGISLCFIGAVGYAAIQESFEEAVCIKITALMANVALIAPLLGPLV
                     GAAWIHVLPWEGMFVLFAALAAISFFGLQRAMPETATRIGEKLSLKELGRDYKLVLKN
                     GRFVAGALALGFVSLPLLAWIAQSPIIIITGEQLSSYEYGLLQVPIFGALIAGNLLLA
                     RLTSRRTVRSLIIMGGWPIMIGLLVAAAATVISSHAYLWMTAGLSIYAFGIGLANAGL
                     VRLTLFASDMSKGTVSAAMGMLQMLIFTVGIEISKHAWLNGGNGLFNLFNLVNGILWL
                     SLMVIFLKDKQMGNSHEG"
     gene            17044..17640
                     /gene="ybjG"
                     /locus_tag="J1Y83_002139"
     CDS             17044..17640
                     /gene="ybjG"
                     /locus_tag="J1Y83_002139"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HBA7630286.1"
                     /translation="MLENLNLSLFSLINATPDSAPWMISLAIFIAKDLITVVPLLAAV
                     LWLWGLTAQRQLVIKIAIALAVSLFVSWTMGHLFPHDRPFVENIGYNFLHHAADDSFP
                     SDHGTVIFTFALAFLCWHRLWSGSLLMVLAVVIAWSRVYLGVHWPLDMLGGLLAGMIG
                     CLSAQIIWQAMGHKLYQRLQSWYRFCFALPIRKGWVRD"
     gene            17698..18456
                     /gene="deoR"
                     /locus_tag="J1Y83_002140"
     CDS             17698..18456
                     /gene="deoR"
                     /locus_tag="J1Y83_002140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor DeoR"
                     /protein_id="HBA7630287.1"
                     /translation="METRREERIGQLLQELKRSDKLHLKDAAALLGVSEMTIRRDLNN
                     HSAPVVLLGGYIVLEPRSASHYLLSDQKSRLVEEKRRAAKLAATLVEPDQTLFFDCGT
                     TTPWIIEAIDNEIPFTAVCYSLNTFLALKEKPHCRAFLCGGEFHASNAIFKPIDFQQT
                     LNNFCPDIAFYSAAGVHVSKGATCFNLEELPVKHWAMSMAQKHVLVVDHSKFGKVRPA
                     RMGDLKRFDIVVSDCCPEDEYVKYAQTQRIKLMY"
     gene            complement(18503..19705)
                     /gene="dacC"
                     /locus_tag="J1Y83_002141"
     CDS             complement(18503..19705)
                     /gene="dacC"
                     /locus_tag="J1Y83_002141"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase"
                     /protein_id="HBA7630288.1"
                     /translation="MTQYSSLLRGLAAGSAFLFLFAPTAFAAEQTVEAPSVDARAWIL
                     MDYASGKVLAEGNADEKLDPASLTKIMTSYVVGQALKADKIKLTDMVTVGKDAWATGN
                     PALRGSSVMFLKPGDQVSVADLNKGVIIQSGNDACIALADYVAGSQESFIGLMNGYAK
                     KLGLTNTTFQTVHGLDAPGQFSTARDMALLGKALIHDVPEEYAIHKEKEFTFNKIRQP
                     NRNRLLWSSNLNVDGMKTGTTAGAGYNLVASATQGDMRLISVVLGAKTDRIRFNESEK
                     LLTWGFRFFETVTPIKPDATFVTQRVWFGDKSEVNLGAGEAGSVTIPRGQLKNLKASY
                     TLTELQLTAPLKKGQVVGTIDFQLNGKSIEQRPLIVMENVEEGGFFGRMWDFVMMKFH
                     QWFGSWFS"
     gene            19952..20578
                     /locus_tag="J1Y83_002142"
     CDS             19952..20578
                     /locus_tag="J1Y83_002142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308945.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HBA7630289.1"
                     /translation="MITLWGRNNSTNVKKVLLTLEELELPYEQILAGREFGINHDADF
                     LAMNPNGLVPLLRDDESDLILWESNAIVRYLAAQYGQKRLWIDSPARRAEAEKWMDWA
                     NQTLSNAHRGILMGLVRTPPEERDQAAIDASCKECDALFALLDAELAKVKWFSGDEFG
                     VGDIAIAPFIYNLFNVGLTWTPRPNLQRWYQQLTERPAVRKVVMIPVS"
     gene            complement(20575..21690)
                     /locus_tag="J1Y83_002143"
     CDS             complement(20575..21690)
                     /locus_tag="J1Y83_002143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HBA7630290.1"
                     /translation="MHRQSFFLVPLICLSSALWAAPATVNVEVLQDKLDHPWALAFLP
                     DNHGMLITLRGGELRHWQAGKGLSAPLSGVPDVWAHGQGGLLDVVLAPDFAQSRRIWL
                     SYSEVGDDGKAGTAVGYGRLSDDLSKVTDFRTVFRQMPKLSTGNHFGGRMVFDGKGYL
                     FIALGENNQRPTAQDLDKLQGKLVRLTDLGEIPDDNPFIKESGVRAEIWSYGIRNPQG
                     MAMNPWSNALWLNEHGPRGGDEINIPQKGKNYGWPLATWGINYSGFKIPEAKGEIVAG
                     TEQPVFYWKDSPAVSGMAFYNSDKFPQWQQKLFIGALKDKDVIVMSVNGDKVTEDGRI
                     LTDRGQRIRDVRTGPDGYLYVLTDESSGELLKVSPRN"
     gene            complement(21801..22184)
                     /gene="bssR"
                     /locus_tag="J1Y83_002144"
     CDS             complement(21801..22184)
                     /gene="bssR"
                     /locus_tag="J1Y83_002144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator BssR"
                     /protein_id="HBA7630291.1"
                     /translation="MFVDRQRIDLLNRLIDARVDLAAYVQLRKAKGYMSVSESNHLRD
                     NFFKLNRELHDKSLRLNLHLDQEEWSALHHAEEALATAAVCLMSGHHDCPTVITVNAD
                     KLENCLMSLTLSIQSLQKHTMLEKA"
     gene            22397..23722
                     /gene="rimO"
                     /locus_tag="J1Y83_002145"
     CDS             22397..23722
                     /gene="rimO"
                     /locus_tag="J1Y83_002145"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HBA7630292.1"
                     /translation="MSKVTPQPKIGFVSLGCPKNLVDSERILTELRTEGYDVVPSYDD
                     ADMVIVNTCGFIDSAVQESLEAIGEALNENGKVIVTGCLGAKEDQIREVHPKVLEITG
                     PHSYEQVLEHVHHYVPKPKHNPFLSLVPEQGVKLTPRHYAYLKISEGCNHRCTFCIIP
                     SMRGDLVSRPIGEVLSEAKRLVDAGVKEILVISQDTSAYGVDVKHRTGFHNGEPVKTS
                     MVSLCEQLSKLGIWTRLHYVYPYPHVDDVIPLMAEGKILPYLDIPLQHASPRILKLMK
                     RPGSVDRQLARIKQWREICPELTLRSTFIVGFPGETEEDFQMLLDFLKEARLDRVGCF
                     KYSPVEGADANALPDQVPEEVKEERWNRFMQLQQQISAERLQEKVGREILVIIDEVDE
                     EGAIGRSMADAPEIDGAVYLNGETNVKPGDILRVKVEHADEYDLWGSRV"
     gene            complement(23769..25097)
                     /locus_tag="J1Y83_002146"
     CDS             complement(23769..25097)
                     /locus_tag="J1Y83_002146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HBA7630293.1"
                     /translation="MSRINKFVLTVSLLIFIMISAVACGIYTQMVKERVYGLKQSVID
                     TAFAVANIAEYRRSVAIDLINTLNPTEEQLLVGLRTAYADSVSPSYLYDVGPYLISSD
                     ECIQVKEFEKNYCADIMQVVKYRHVKNTGFISFDGKTFVYYLYPVTHNRSLIFLLGLE
                     RFSLLSKSLAMDSENLMFSLFKNGKSVTGDEYNAKNAIFTVSEAMEHFSYLPTGLYVF
                     AYKKDVYFQVCTLIIFFAALVAVISGASCLYLVRRVINRGIVEKEAIINNHFERVLDG
                     GLFFSAADVKKLYSMYNSAFLDDLTKAMGRKSFDEDLKALPEKGGYLCLFDVDKFKNI
                     NDTFGHLLGDEVLMKVVKILKSQIPVDKGKVYRFGGDEFAVIYTGGTLEELLSILKEI
                     VHFLVGSINLSTSIGVAHSNECTTVERLKMLADERLYKSKKNGRAQISWQ"
     gene            complement(25105..>26672)
                     /locus_tag="J1Y83_002147"
     CDS             complement(25105..>26672)
                     /locus_tag="J1Y83_002147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HBA7630294.1"
                     /translation="VPNNALFEIYFYYPGGNLLNASDKLFYLPFAFIIIVLLVVYLMT
                     TRVFRRQFSEMTDLANTLAFLPDSTDQIEALKIREGDAKEIISIKNSIAEMKDAEIER
                     ANKLLSLISYDQESGFIKNMAIIESNNNQYLAVGIIKLCGLEAVEAVFGVDERNKIVR
                     KLCQRIAEKYAQCCDIVTFNADLYLLLCRENVQTFTRKIATVNDFDSSFGYRNLRIHK
                     SAICEPLQGENAWSYAEKLKLAISSIRNHMFSEFIFCDDAKLNEIEENIWIARNIRHA
                     MEIGELFLVYQPIVDINTRTILGAEALCRWVSAERGIISPLKFITIAEDIGFINELGY
                     QIIKTAMGEFRHFSQRASLKDDFLLHINVSPWQLNEPHFHERFTTIMKENGLKANSLC
                     VEITETVIERINEHFYLNIEQLRKQGVRVSIDDFGTGLSNLKRFYEINPDSIKVDSQF
                     TGDIFGTAGKIVRIIFDLARYNRIPVIAEGVESEDVARELIKLGCVQAQGYLYQKPMP
                     FSAWDKSGKLVKE"
BASE COUNT         6841 a         6916 c         6354 g         6561 t
ORIGIN      
        1 ccaaaaatca taagattgac agacgggagt tccatcatga aaaagttagt tcttgccgca
       61 ttacttgctt cttttacttt cggtgcttct gccgcagaga aaatcaattt tggcgtttca
      121 gccacctatc ccccctttga atctataggt gctaataatg agattgtcgg ctttgatatc
      181 gacctggcaa aagccttgtg caaacaaatg caggcagaat gtacttttac taatcacgct
      241 ttcgacagcc tgatcccgtc actgaaattc agaaaatatg acgccgtaat ctccggaatg
      301 gatatcacgc cggagcgtag caaacaggta tcgtttacca cgccctacta tgaaaactca
      361 gccgtcgtga ttgccaaaaa agatacctac aaaacgtttg ccgatctgaa aggcaaacgt
      421 attgggatgg aaaacggcac cacgcaccag aaatatattc aggatcagca cccggaagtg
      481 aaaactgtct cctatgacag ttatcagaat gcctttatcg atctgaaaaa tggccgtatt
      541 gatggcgtat ttggtgacac cgcagtggta aacgaatggc tgaaaaccaa tccgcaactg
      601 ggcgttgcta ctgaaaaagt gaccgatccg caatacttcg gcaccggcct gggtatcgct
      661 gtacgtccgg ataacaaagc tctgctggaa aaactgaata acgcgctggc agcaattaaa
      721 gctgacggta catatcagaa aatcaatgac cagtggttcc cacagtaaga ttcagaaagc
      781 cgcacctgcg gcttttttat tgcttcacca gtagcgtcag cacttcatag tgcgcggtgt
      841 gcgggaacat atcaaaaagc tgtacccgtt cgatgcgata accaggcagt tcgcggatat
      901 ctttcgccat agtttgggcg ttacagctgg agtagataat aaaacgcggt gccatcgttg
      961 agagataatc acacagcggt ttaccgatgc cgcggcgcgg cgggttaacc agcaccagct
     1021 ccggcacttc cccctgagcg gtggcaaact gcgtagagtc cagcgcctga aattgcaaac
     1081 gcgttaagcc cagttcagcg gctgactgct tcgcacaggc aatggcctct ggtgcaattt
     1141 cgatcccggt taactgcatg tcaggcgtcg cgcagtgtaa accaaagcca cccacaccgc
     1201 agaacagatc ccacatatgt ttaaccggca gctgtcgtac ccagtcgcgc gcggtggcgt
     1261 acagctggct ggcgaccgcc ggattagtct ggaagaaact ttgcggacgg atccacagcg
     1321 gtacgtcatt aaaacgctct gccagtgcct gttgttcggt caggtagatc tccgtctccc
     1381 cttccataat cgccatatgt accggctgaa tattgacggt aataactttc agctgcggta
     1441 attgttcctg taaccacggc agcgccttac gcagttgcgt cagtttggta tcagaacgca
     1501 gtacaaaacg cagcatcatg ccgccatcgc tctggctttc agtcagcaga atgtatttca
     1561 gttcaccacg tttacgcgcc acgttgtagg gcgttaaccc cgcacgggcg ataaacggtt
     1621 ttagcgccgc aaaaacgggc gcaaatgagg ctggataaag cgggcagtca caaaggtctt
     1681 ccggtgtacc atcccgatgc agcataccga gcagtggttt ttcaacgcta ccactcacca
     1741 ccattttggc tttattacga aacccttgtt ccgggcctga caccggcgca caccactcct
     1801 caaccggaaa atcggcgagc agatttttaa gatcggcggt tttagcggag agttgctctg
     1861 gaatcggctg cgttatccac tgacaggaac gacagcgacc cgcgtcgtaa agtgcgcact
     1921 gcatacattg accttcacat catcaggggc gacgattata cacattattg cagctggaag
     1981 aaccgccgac tggtcgaggg aacgaacagc agcatcagga tgagcatatc cggcagcttc
     2041 tgcagcatca ggctatggaa gatttcacgt tttgattcac cgggaatgct gaacagttcc
     2101 ggataaccat accccagcga agccgcccac aagtaacttg cggctgtgat ttgtgtcaac
     2161 agatacagcc agcgcgccca gcgacgccct ttcaccagtg aaaacgcaca ccagatctca
     2221 ataaaaacca gcaccagact gctcaaaaag accagcgtta aactccaggt ttgtacgctg
     2281 cgatgaatga actcgccaat accgcgcacg cccagcgtat tgaaaatcat cagcacgtcg
     2341 aggccacgga tcataataat ggcgagcgcc gccacctgca ccagcgcagg cacattcagg
     2401 cgagcatgag atgaagatgt tttcttaaaa aatcccaacg tttcgtcttc catgaaaaca
     2461 atgccgcgac atgcgcggca ttatgtagcc aggttggcga attttagtgt cttcagccac
     2521 gtcttgcacg ctggatgtcg cgtatccgct gtttttctgc gcgcgccatc agataccagg
     2581 cgataaatcc gacaattccg accgcgccga ggattaatgt tgccagggcg ttgatttccg
     2641 gattcacccc catccgcacg ctggaaaaga ccagcatcgg taacgtggtg gctcccggcc
     2701 cggaaacaaa gctggcgatc accagatcat caagcgacaa agtaaaagcc agtaaccagc
     2761 cagaaatgat cgcgggcatg atcatcggta gcgtaatgac gaaaaacact ttcagcggcg
     2821 tcgcaccaag atccatcgcg gcttcttcta tcgagcaatc cagttcccgc agacgcgacg
     2881 aaataacgac tgccacataa gccgtacaga aagtgacatg cgccagccag atggtgagca
     2941 taccgcggtc cgcaggccag ccaatggcat gagcaagcgc gacgaataac aacaacagcg
     3001 acaaacccgt gatgacatct ggcatcacca gcggcgcggt gatcataaag gcaaaaccat
     3061 ttgatccgcg aaacctgcca aaacgcacca acaccaccgc cgcaatcgtc ccgaggatcg
     3121 ccgctgccgt tgccgcacag gctgcaattg tcagacttaa accaaccgca ctcatcatcg
     3181 cgtcatcgcg caataattcg ccataccagc gcgttgacca gccggcccac accgtcacca
     3241 gtttcgagct gttgaatgaa tagataacca gcatcagcat tggcgcgtag agaaaggtga
     3301 agcccagcag caaaatcaca atccgccagg gcgaacgaac aaccggtaaa ttattcatcc
     3361 gtgttctccc acgctttttt gctggtgttt gtgaaaccac attatcggca caattagcag
     3421 cagcaacatg atgatcgcta ccgccgaggc taccggccag tcgcggttgt taaagaactc
     3481 ctgccacagc acgcgcccga tcatgatgct gtccgggcca ccgagcagtt ccgggatcac
     3541 aaactcgccc acagcaggga taaacaccag catcgatccg gcaataatcc cacctttagt
     3601 cagcggcacg atcacagtaa agaacgtttt cagcggtcgt gcaccgagat ccagtgccgc
     3661 ttctaccagc gaataatcaa tacgaatcaa cgcggtgtaa atcggcaaca ccataaacgg
     3721 cacgtaggcg taaacaatgc caatataaac cgcaaagttg gtatgcagaa tggtcagcgg
     3781 ttgatcgata acccccaacc acagcagaaa attattcagc acaccgttgc tttttaatat
     3841 tcccatccag gcgtagacac gaatcagaaa cgaggtccac gacggcagga tcaccagtag
     3901 taataagata ttacgggtcg aaggcttact gtgcgccacc gcccacgcca gcggatagcc
     3961 gatcagtaaa cagcaaatag tcgaaatcgc cgccacctgt aacgactgga gataagcatc
     4021 aaaatagagc ggatcgtcgg tcagttgcag aaaattaccg agattaagag tgatagaaag
     4081 ttgcccgtca gcccactcca tcaattcggt ataaggtgga atagcgcgca ccatctccgc
     4141 caggcttatt ttaaagacga tcagaaatgg cagcagaaac agcaagatca accagatata
     4201 tggcaacgca atgaccagtt tgcgcccatg cttcatttgc agctgcgaca accacagctt
     4261 aaagggtttt ggctgggcag caggttcaag tgtactcatc tttgctcctt aaaccgtcag
     4321 caccacacag ctgtccactt cccagcacaa acgtacttcg tcgccccagg tcggtaaccc
     4381 tttacgatgg cgatgggcgt tttgtagctg ggcgctaatc atctgcccac ttttcagacg
     4441 aacgtgatac accgaaagat cgccgagata ggcaatgtgt atcacctccc ccaccgcgaa
     4501 gttacaacca ttggcgggcg gttcctcgca aagcatgatt ttttccgggc gaagcgccac
     4561 atgcaccggc acgttatcaa caaccgaagc atccacatcg actttcagtg gatgcaccag
     4621 ccccggcgaa taaagcacca gaccatcttc ctgacgctct ttgagtacac cttcaaagac
     4681 attaaccgaa ccgataaatt cagcgctgta gcgagtggtc ggatgctcgt agatctcttc
     4741 cggctcgcca atctggacaa atttcccacg attcataatg gcgatgcgcc ccgccatagt
     4801 catcgcctct tcctgatcgt gagtaaccat tacgcaggtc acaccgacac gctccagaat
     4861 atccaccact tcaagctgca tccggtcacg cagcttttta tccagcgcgc ccatcggctc
     4921 atcgagcagt aatagtttcg ggcgcttggc aaggcttcgg gccagggcca cacgctgacg
     4981 ctgaccacca gaaagctgat gcggtttgcg tttggcgaac tcctgcatat gcaccagccc
     5041 aagcatctca ttgacccggc tggcaatttc cgctttcggt agtttgtcct gtttcaggcc
     5101 aaaagcgatg ttctgttcca cggtcatatg cggaaacagc gcgtaagact gaaacatcat
     5161 attgatgggg cgcaggtaag gcggcacctg tgacaaatcg acgccatcga gcataatctg
     5221 cccggcagaa ggttgttcaa aacctgccag catacgcagc agcgtggact tgccacagcc
     5281 ggatgcgccc agcagcgcga agatttcacc tttgtagatg gtcaggctga catcatccac
     5341 cgcatgttga ccatcgtagg atttggtcag gttacggatt tctaatagcg gcgtcagcgc
     5401 cttacgggtt ttcgcctgcg ggcgagggat agcgtcattc acggctgctc tccggcattg
     5461 aatgtgtgca agcgcaccat gatggtgcga attgccggcg ggtgtggtgc gcccgtccgg
     5521 catctacggc tgcggattat tttccgctct tcactttggt ccacgcgcgg gtgcgcacac
     5581 ggtcgatttt cgggtcctgc actttcagag tgaacagctt cgcacgaaca tccgcaggcg
     5641 gataaatacc cgggttatca cggacttccg cactcaccag cggcgtggct tctttattag
     5701 cattagcata gaacacatgg tcagagatat gtgctaccac atccgggcgc agcagataat
     5761 tcaggaactg ataggcttcg tctttatttt tggcatccgc aggcatggcg aagacatcaa
     5821 agaatgccat cgccccttct ttcggaatcg agaacgagac attcacgcca ttcttcgctt
     5881 ctttcgcgcg gtttgacgcc tgccagacat cgcccgccca gccaatagcg acacagatgt
     5941 caccattcgc cagatcattg atgtattggg atgaatggaa ataacgaatg ttcgggcgca
     6001 gctttaacag cagatcggta gccggtccgg tgtaatcatc cgctttagtg ctgttaggat
     6061 ctttaccgag ataattcagc acggtagcaa acacttcttc tggcgcatcc aggaaagaga
     6121 caccgcagct tttcagtttt tccagatttt caggtttgag gatcaagtcc cagctatcga
     6181 cgggcgcgtt ttcgcccaac accgctttaa ctttatcgac gttatagcca atcccggtcg
     6241 tcgcccacat atagggcata gcaaatttat tgtcgggatc gtgtttggcg accaacttca
     6301 gcagctccgg atcgagattc ttccactccg gcaatttgct tttatccagc ggctggaaaa
     6361 ctcccgctgt cagctggcgc tcaagaaagc ttgctgacgg caccaccaga tcaaagccag
     6421 tgctccccgc cattaatttg ccttccagta cttcgttaga atcgaaaaca tcgtagacga
     6481 ctttaatacc ggtttctttt tcaaagttgg ccaccgtgtc cggggcgata taatcagacc
     6541 agttataaat gtggagtgtt ttttgttcag ccgcgagcgt gctgacagag acggccatca
     6601 gagcccccgc aaccagaccc gatagccatt ttttatttaa ggcggtcata tctctttcct
     6661 tctgaaaatt cgttaacaaa cgaacaaaag ttgtcacact gaaagatatg caataaacgt
     6721 gcatattttt atcggcgcgg tgtaagaaaa gagaactctc tcccggcctg cactgctcac
     6781 tatataaaag catcgcaaga ataactttag ccagggtttg agactatagc tcggattaaa
     6841 aaacggtatt taacaaattt ttatgcgtat ttgctctatg tgataaagcc aaatcggcac
     6901 aagagaagtg aattaatctt taataaaagg ttaaaaacag cggataattt tgcggcacgc
     6961 agtcactgcg gcagcgactg cgtgctcgaa aggagttatc agtggaggaa atagttttcg
     7021 gtaacgttat tttgcccttc atcgtcagtc ggtaacagca gttgatgggc attagcttca
     7081 agaatgacca tcgaaatctg ctcttcgctc tgacgcacga accatgcaaa ctgctcatag
     7141 gttacgccct gcataacgga taaagactgg caaaccacca gctttggcag attatcatcc
     7201 tgcatatcaa gaaatgcttt cacggtcagc gaactggcat tgatggctga taaatccgcc
     7261 gccagcggca atacggctga aggtcggact tccgccatgg cagaaaacag gatcgtgtta
     7321 tcgatcagat cgattttagc atcaaagaca ccgtcgaaat tctgcatatg gggcagatgc
     7381 agagcctgac agttatcaca ttcaaaaaaa ctcatcccca ggtcatcgag ccattgacgc
     7441 aacgtatcca gaccaggaac gaccagcgat gtcataattt tgtacaacct cttccgataa
     7501 aaagaccggc acagcttacg caaaaagcgc aggcaaaacc atgatcagta atgtgattgc
     7561 gactaaccac ccgttttcag gcaatattct gtcgtagcgt agcgttcgat ccagcggatc
     7621 attttactcg cgatgtcgat accggtggtt ttttctattc cttccagccc tggcgacgca
     7681 ttcacttcca tcaccaacgg cccgcgatta gcacgcagaa tatccacacc agcaacgtcc
     7741 agcgccatcg ttcgcgcggc ttttatcgcg atttcacgct cctgtggtgt gatactggcg
     7801 acgcttgctg cgccgccacg atgcaaattg gaacgaaaat cgccctcttt cgcccgccgt
     7861 tcaatcgcag cgactacttc atcgccaaca accagacagc ggatatcgca cccttgcgcc
     7921 tctttgatat attcctgcac cagaatatgc gcgttcagac cgcggaaagc gtcaatcacg
     7981 ctttccgccg cctgacgcgt ctccgccagc acgacgccaa ttccctgcgt gccttcaacc
     8041 agcttgacca ccagcggcgc accaccgacc atgtcgatta aatcgctggt atcatccggc
     8101 gaatgcgcaa tgcccgtgac aggcaggtcg atgccctgac gcgccagcag ttgcatggaa
     8161 cgcaatttgt cacgcgcccg ggcaatggcg accgactcat tgagcggata gctccccagc
     8221 atctcgaact ggcgcagtgc cgccgtccca taaaaggtaa tggcggtgcc aatacgcggg
     8281 atcactgcgt caaaatgggg taacttgcgg cctttgtagt gaatagaaga cgccgcagga
     8341 tttatgttca tgtagcaaga aagcggatca agaatttcaa ccaggtgacc gcgctgtata
     8401 gcggcttcac gcagccgctt acacgaatag agcgttccat cccgggacaa tatggcaatt
     8461 ttcaccctgc acctctctgt cagacctggt caaaagcccg cgtatcatac atcgacgtgg
     8521 cagttttagc gcgtcgccca accctgtttg tgcaaataat ccagaataaa tgggcggctt
     8581 tctttaatga ttgttcggcg gatatgatcg ctccaggtat cccggcgatt attgctgcca
     8641 cgggtgaggt aatattccgc cagttgctcg tcatactgcg ccagtgcgtc tttatccagc
     8701 ggttgatagc tgttttcatg caccaaaatg gaggccggta aacgcggctt aagatccgga
     8761 ttatccgcag gccagccaag gcacagccca aacagcggca gaacatgctg cggtaatttc
     8821 agcagttccg tcaccgcttc aatattattg cgcaggccgc cgatatatac cccacccaat
     8881 cccagcgatt ccgctgcggt taatgcattc tgcgccatca ttgccgtatc aacgacaccg
     8941 agcaacagtt gttccgccag gccgagctga gcatccggac agatctgtaa atagcggtta
     9001 aagtcagcac agaacaccca aaactccgcc gcttgcgcta cgtgtttttg cccgccggtc
     9061 agcgtcacca gttcttcacg taacgctttg tcggtaatgc gaataatgct actgcactgc
     9121 aaaaaactgg aactggacgt cgcacgggcg ctgttaataa tcgcctcacg ctgcgcttcg
     9181 gaaatgggtt catcagtgaa atggcgaatg gagcgatggc cacaaataag ttcaatggtt
     9241 ggcgtcatta tctttttctc tttctgaacg tgaatattgc ggtggatggt tcatcagctg
     9301 tggggcaaga cgttttgcca cctgaagaat aaccaccacc gcagcgggaa gcatgagcaa
     9361 aacaccgaga aaaatcatca gaatctgcac ttctggccga gaaaatggct caggtagcga
     9421 cagggagtcg cttaccgaca gcagcgccac cgccagcaac atcattccga taaattccag
     9481 tatcaacacg cttttaggca atttaccgat cgcgcgcata cgcttccctc tgcaaagtga
     9541 gccttcagtc taaaactttt cactgtattg tgtttaacag ttatagcttt tatcaattaa
     9601 tgcaacaggt taaacctact ttcagcgaat acattttagc gtgatcatta caggcataaa
     9661 tctatgagga gagaaataat gcaaaccgtt atttttggtc gtccgggttg cccttactgt
     9721 gtgcgtgcaa aagatctggc tgagaaattg agcaatgaac gcgatgattt ccagtatcag
     9781 tatgtagata ttcgtgcgga agggatcact aaagaagatc tacaacaaaa ggcaggtaaa
     9841 cccgtagaaa ccgtgccgca gatttttgtc gatcagcaac atatcggcgg ctataccgat
     9901 tttgctgcat gggcgaaaga gaatctggac gcctgatcgt ctgacaagcc ctcgcgttga
     9961 gggctttatt gattttttct gtgctgtggt ttaaacaaac tactgataaa taagaaacac
    10021 aatgccccca gcgcacacca gaacaccgca cttaataacc atgccagctc ttgccagaat
    10081 gagcgcgtcg gtgaaaaaaa cagccgcata atgagcatcg aacagggtgc cgccagcatt
    10141 gcgccaaaca gaggtttcag gacttctcta cgatgtgaaa agaagctggc aactgcacca
    10201 ggaagaatga aaaatagcaa accgatttca ggatgcccgg cagcccgaaa agcgcctttc
    10261 atgtgcgtcg ccagaaaaag acacaccaca atgaagagga caaaacagca gattgccccc
    10321 gcccaacgtt gtttatgttt cactcgttcc tcctgacact gcgtctatcg aacacatttt
    10381 tcgccagtgt ggcgttcagt aagataaagc cgcttcgcat tccatgctaa tataggccaa
    10441 cgcaattcat atagccgttg atacctaatg tgattacact agtaaaatat attgttactt
    10501 tactatcgtt taggtgcgct gaatgaatct gcgccctgaa ttctggtaaa aaacattatc
    10561 gtaaattacc atttctttca acagcttact agtaaacaag aagttagcct ccgtgaatat
    10621 aaacgtcgcc gaattgttaa atgggaatta cattctgtta ttatttgtgg tcctcgcgct
    10681 tgggctatgt ctcgggaaat tacgacttgg ttcgatccaa ctgggtaatt ccattggcgt
    10741 tttagtcgta tcgctgttat taggccaaca acatttcagc attaacaccg acgcgcttaa
    10801 tcttggcttt atgctgttta ttttctgcgt cggggtcgaa gccggaccga actttttttc
    10861 catttttttt cgcgatggga aaaattacct aatgttagca ctggtgatgg ttggcagtgc
    10921 gctggtgatc gccttagggt taggtaagct gtttggctgg gatattggcc tgacggccgg
    10981 tatgttagct ggctctatga cgtcgacacc ggttctggtc ggtgctggcg atacactgcg
    11041 tcattccggc atggaaagca ggcagctctc actggcactg gataatctga gcctcgggta
    11101 tgccttaacc tatttaatcg gtctggtgag tttgattgtt ggtgcgcgtt acttgccgaa
    11161 attgcagcat caggacttac agaccagcgc ccagcaaatc gcccgcgaac gtggcctgga
    11221 cactgatgcc aaccgtaagg tttatttacc ggtgatccgc gcctaccgcg tcggcccgga
    11281 gctggtggcc tggaccgacg gcaaaaatct gcgtgaactg ggtatttatc gacaaactgg
    11341 ctgctacatt gaacgtattc gacgtaacgg gattctggcg aatccagacg gtgatgctgt
    11401 gctacaaatg ggcgatgaaa tagcgttggt aggctatccc gacgcccacg cacgactcga
    11461 tcccagcttc cgtaacggca aagaagtttt cgatcgtgac cttctcgaca tgcgtatcgt
    11521 cactgaagaa gtggtcgtta aaaaccataa cgccgtgggc aaacgtctcg cacaactgaa
    11581 gttgaccgat cacggttgct tccttaaccg cgtcattcgt agccagattg agatgccgat
    11641 agatgacaac gtcgtgctta acaaaggtga cgttttacaa gtcagcggcg atgcccgtcg
    11701 cgtaaaaacc atcgccgatc gcatcggctt tatctcgatt cacagccagg tcactgactt
    11761 gctggcattc tgcgccttct ttgttattgg gctgatgatc gggatgatca ccttccagtt
    11821 cagcacattc agtttcggca tggggaacgc tgccgggttg ttattcgccg gaattatgct
    11881 gggatttatg cgcgctaacc acccgacctt cggttacatt ccgcagggtg cattaagcat
    11941 ggtgaaagag ttcggcttaa tggtgtttat ggcaggcgtt ggtctgagcg ccggtagcgg
    12001 tattaataac ggcctgggcg cgattggcgg tcagatgttg attgccggat tgattgtcag
    12061 tcttgtgcca gtggttatct gtttcttgtt cggtgcttat gtattgcgaa tgaaccgcgc
    12121 actgttgttc ggcgcaatga tgggcgcacg cacctgcgcg ccggcaatgg agatcatcag
    12181 tgatacagct cgcagtaaca tcccggcgct gggctatgcg ggcacctatg caatcgccaa
    12241 cgtcctgctg acgctggcag ggacaatcat cgtcatggta tggccaggat taggataaaa
    12301 ctgaagttgc cctgaaaatg aaattttttt tgcacaaccg cagaactttt ccgcagggca
    12361 tcagtcttaa ttagtgccac tgcttttctt tgatgtcccc attttgtgga gcccatcaac
    12421 cccgccattt cggttcaagg ttgatgggtt ttttgttgcc tgaaatttat ctactaccca
    12481 gcaatcctct caaccatcct caaaatctcc tcgcgtgata gcggcttacg gtcggtgaca
    12541 aaatgcagcg tcatcccctc aataaacgca tcaagcgtgc gggcggttcc gggttcaaac
    12601 cattgttcga gcgtttgttg actgcgctgc atccagtttt gcattaccgt ttttaagagc
    12661 ggttttcggc tagccagcgc gtagagctgg tacatcagct ccatattatc cggcgttgcg
    12721 acctgtgagc tgtagatcat atcggtgata gcctggcatg cgcctggagc atccctaaca
    12781 tcgctaaaaa atgcctgata ttgccgggac atgatctcag taaaacggct gaacgcctcc
    12841 agcaacaact catcaatgcc tgaaaaatag taggtcatcg atcccaacgg caccccggca
    12901 agggtagcaa ttttgcggtg tgtaacagca tgtattccgt aaagtttcac cgcctccagc
    12961 gtggcctgga taattttttc tcgccgttgc ggatcgttag cgcgacgcat gtcatttcct
    13021 ctctctgcaa ttgtgtacaa atgtacacag tcttgctaat gttgtcttcc ctcttcttat
    13081 tttgacgtgg tctatgaccg taaattcttc acgtaatgca ttgaaacgcc gaacctgggc
    13141 gctgtttatg ttcttctttt taccaggcct gttaatggcg tcctgggcaa cccgtacgcc
    13201 tgctatccgc gatattctct ctgtctcgat cgctgaaatg ggcggtgttc tctttggtct
    13261 gtcgatcggt tcgatgagcg gtattctctg ctcggcgtgg ttagtgaaac gctttggcac
    13321 gcgtaatgtc atcctggtca cgatgtcctg cgcattgatc gggatgatga tattaagtct
    13381 ggcactctgg ctaacatcgc ccctgctctt tgccgttggt ctcggcgtct ttggggcaag
    13441 ttttggttct gcggaagtgg cgataaacgt tgaaggtgct gccgttgagc gagaaatgaa
    13501 taagacggtt ttgccgatga tgcacggttt ttatagcctg ggcacgctgg caggcgctgg
    13561 tgtcgggatg gcactgacgg cctttggcgt tccggcaacg gtgcacattt tattggcggc
    13621 gctggtaggc atcgcgccta tttatatcgc cattcaggca atccctgacg gtacgggcaa
    13681 aaatgctgcc gatggcaccc agcatggcga aaaaggcgtt cctttttatc gcgatatcca
    13741 gttgctgttg attggtgttg tagtgctggc gatggccttt gccgaaggtt ctgccaacga
    13801 ctggttaccc ttattaatgg ttgatggtca cggttttagc cctacttccg gctcgctgat
    13861 ttatgccggt tttaccctgg ggatgaccgt tggacgcttt accggcggtt ggttcattga
    13921 ccgttacagt cgcgttgccg tggttcgggc cagtgcacta atgggggcgt tgggtattgg
    13981 gctgattatt tttgtcgata gcgcctgggt tgctggggtg tctgttgtac tttggggact
    14041 gggtgcctcg ctgggcttcc cgctgaccat ttctgccgcc agcgataccg gccccgatgc
    14101 accaacccgc gtcagtgtgg tagcaacgac cggttatctg gctttcctcg tcgggccgcc
    14161 gctgctgggc tatctcggcg aacattatgg attacgtagt gcaatgctgg ttgtactggc
    14221 gctggttatt ctcgcggcta ttgtcgcgaa agccgtcgcc aaacccgata ccaaaacgca
    14281 gacggcgatg gagaatagtt gatgagcatt aaattaattg cggtagacat ggatggtact
    14341 ttcttaagcg atcaaaaaac ctataaccgt gagcggttta tggctcagta tcagcaaatg
    14401 aaagcacaag gcattcgctt tgtggtcgcc agcgggaatc aatattatca gttgatctct
    14461 ttcttccctg aaattgctaa tgaaatagcc tttgtggctg aaaacggcgg ctgggtagtg
    14521 agcgaaggca aagatgtttt taatggcgag ctgtcgaagg atgcgtttac tactgtcgta
    14581 gaacatttgc tgacgcgccc ggaagtggaa attattgcct gcggaaaaaa tagcgcctat
    14641 accctcaaaa aatatgacga tgccatgaaa acggtggcgg aaatgtatta tcaccgtctg
    14701 gaatacgtcg ataactttga caacttagaa gatatcttct ttaaatttgg ccttaatctc
    14761 tctgacgaac tgatcccaca agtgcaaaaa gcattacatg aggccatcgg cgatatcatg
    14821 gtaccggtcc ataccggcaa cggcagcatc gatctgatta tccccggcgt acataaagcc
    14881 aatggccttc gccaactgca gaaattatgg ggaatagacg acagcgaagt ggtggtcttt
    14941 ggcgatggcg gtaacgatat tgagatgctg cgtcaggcag gctttagttt tgcaatggaa
    15001 aatgccggca gcgcggtcgt cgcagcggca aaataccggg caggctccaa taaccgtgaa
    15061 ggcgtactgg atgtgatcga taaagtgctt aaacacgaag cgccatttaa ccaataaaac
    15121 ggcagcacgc ccggcaacac aatgcctgaa tgaagattgc ataattcgcg gaaattaacc
    15181 cgctgcatta tttcaggcga aattattatg aaaaattgtc tactgttgga cgcgctttta
    15241 atgggcttta ctggtgtggc gatggcgcaa agtgtcaccg tcgatgtgcc gagtggatac
    15301 aaagtggttg tggttcctga ttcagtcagc gttccacaag cggtcagcgt cgcgactgtg
    15361 ccgcaaaccg tctatgtggc tcctgcgcca gctccggctt accgcccgca tccttatgtt
    15421 cgccatcttg ccagcgtagg tgaagggatg gttatcgaac accagattga cgaccatcac
    15481 cattaattcg agaatgcctg atcgcacaaa gcaatcaggc atttttttac ccttcgtgag
    15541 aatttcccat ctgtttatct tttaaaaaga taaccatcag cgacagccac aaaatgccgt
    15601 tgacaaggtt gaagagatta aacagtccgt tgcccccgtt cagccaggca tgtttgctga
    15661 tttcaatacc gacggtaaag atcagcattt gcagcatccc catcgccgca gaaaccgtac
    15721 ctttactcat atcgctggca aacagggtta atcgcaccag tcccgcattc gccagaccaa
    15781 taccgaaagc ataaatactt aacccggcgg tcatccataa atacgcgtgc gatgagataa
    15841 ccgttgccgc agcagcgacc aacagaccaa tcataatcgg ccagccgccc ataataatca
    15901 gcgaacgtac ggtgcggcgc gaggtcagac gcgctaacag caagttaccc gcaattaacg
    15961 ccccgaaaat aggcacttgc agcaagccat attcatagct gctcaactgc tcaccggtaa
    16021 tgatgataat cggcgactgg gcgatccacg ccagcaacgg caggctaaca aatcccagcg
    16081 ccagcgcccc cgccacaaag cggccgttct tcagcaccag cttatagtca cgaccgagtt
    16141 ctttcagcga cagtttctcg cctatacgcg tggcggtttc aggcatagct cgttgcagac
    16201 cgaaaaagga gatcgctgcc aatgcggcaa acaagacaaa catcccctcc cagggcagca
    16261 catggatcca cgccgcgccc accagcggac caagtagtgg agcaatcagc gccacgttcg
    16321 ccattagcgc ggtgatcttg atacaaaccg cctcttcgaa ggattcctga attgcggcgt
    16381 atcccacagc gccaatgaaa cagaggctta tgccctgcaa gaagcgcaac agggtgaatt
    16441 gttcaatgtt ttgcgccagt aatattgcca gacaggtgac gataaaccac accactcccg
    16501 ccaacatcac cggacggcgg ccaatacgat ccgacaacgg ccccaggagc cattgtaaaa
    16561 acatcccgcc cgccagatac gcagtcatcg aagtaggaac ccaatcaatg cccgcctgat
    16621 attgttccac cacggccaac ataccgggtt gaatcatatc gttgccgata taggttgaaa
    16681 attcgtaaag caccagacag agagggaaaa gtaacgcctg acgtccaagc ctggcaccgg
    16741 aagctaattt attttgcatg caatttcttc gccaataata atcgcgcaaa gtttaataaa
    16801 agcgcagcta acgagaaagc gaattttgta gctgaaacca cggttaagca cattcttaca
    16861 ttattgcgag tatagctacg ctttctttaa gttttattta acctttgccc gttacaatca
    16921 cctaccgtaa acaggccgct tgagggaaat aagacgatgc cgctttaccc agtttaacct
    16981 gcactttatt ctcaacgact tgcctgtatt ggctcccttt taatcacttt gcgtcgggaa
    17041 gttatgctgg aaaatttgaa tctctctcta ttctctctta ttaacgcaac gccagactct
    17101 gcgccgtgga tgatctcgtt ggcgattttt attgctaaag atttgattac cgtggtgccg
    17161 ttgctggccg cagtactttg gttgtggggg cttacagcac aacggcaact ggtgataaaa
    17221 atcgctatcg cgctggcggt cagcctgttt gtgtcctgga cgatgggaca tctttttccg
    17281 cacgaccgac cctttgtcga aaatatcggc tataacttcc tgcatcatgc ggcggatgac
    17341 tcattcccga gcgatcacgg tacggtgatt ttcacctttg cactggcatt tttatgctgg
    17401 catcgcctgt ggtccggctc acttttaatg gtgctggccg tcgtcattgc ctggtcgcgc
    17461 gtttatcttg gcgtccactg gccgctggat atgctcggtg gattgctggc aggtatgatt
    17521 ggctgcctta gcgcccagat tatctggcaa gcgatggggc ataaactcta tcaacgtctg
    17581 caatcgtggt atcgcttctg ttttgcatta ccgatccgca aaggctgggt gcgtgactga
    17641 tctctgctaa aaagtgtagt attgagcggc tcgcttcaat aactattcag agggattatg
    17701 gaaacacgtc gcgaagagcg tatcgggcag ctgctgcaag aattaaaacg cagcgataag
    17761 ttacatctta aagacgccgc cgccctgctt ggggtttcgg agatgacgat tcgtcgcgat
    17821 ctgaacaacc acagtgcgcc cgtcgttttg ctcggcggct atattgttct ggaaccacgc
    17881 agcgccagcc attacctgtt aagcgatcaa aaatcccgcc tggtggaaga aaaacgccgg
    17941 gcggcaaaac tggctgcgac gctggtagaa cccgatcaga ccctcttttt tgactgtggc
    18001 accaccacgc cgtggattat tgaagcaatt gataatgaaa tcccttttac cgccgtttgt
    18061 tattcgctaa atacttttct ggcgctgaaa gagaaacccc attgccgcgc gtttctttgc
    18121 ggtggtgaat ttcacgccag caacgccatt ttcaaaccca tcgattttca gcaaacgctg
    18181 aataattttt gcccggatat cgctttttat tccgctgcgg gcgtgcatgt cagtaaaggc
    18241 gctacctgct ttaatcttga agagttgccg gtaaaacact gggccatgtc gatggcgcaa
    18301 aagcatgtgc tggttgtcga ccacagtaaa tttggcaagg tgcgtccggc gcgcatgggt
    18361 gacctgaaac gctttgatat tgttgtgagc gattgttgcc cggaagatga gtatgtgaag
    18421 tacgcgcaga cgcagcgtat taagttgatg tattaatgac gtataaccgg atgacgtttc
    18481 gcgccatccg gttatcagaa gattaagaga accagctgcc gaaccactga tggaatttca
    18541 tcatcacgaa atcccacatc cgaccaaaga atccgccctc ttccacattt tccatcacga
    18601 tcagcggacg ctgctcaatg gatttaccgt taagctggaa atcaatggtg ccgacaacct
    18661 gacctttttt cagcggtgcg gtaagctgca gttccgttaa cgtataactc gccttcaggt
    18721 ttttcagctg cccacgcggt atggtcactg agcccgcttc gcctgccccg agattcactt
    18781 cgctcttatc accaaaccag acgcgctgag tcacaaatgt ggcatcaggt ttaattggcg
    18841 tcacggtttc aaagaagcgg aaaccccagg tcaataattt ctcagactca ttaaaacgga
    18901 tacggtcagt tttcgccccc agcactacgg agattaaacg catatcgccc tgggtagccg
    18961 aagcaaccag attatatccc gcgcctgccg tggttcctgt cttcatgcca tcaacattca
    19021 gattgctgct ccacagcaga cgattacggt taggctgacg aattttgttg aaggtgaact
    19081 ctttctcttt atgaatggcg tactcttccg gcacatcgtg gatcaatgct ttacccagca
    19141 atgccatatc gcgcgcggta ctgaactgcc ccggcgcatc caggccgtgc accgtctgga
    19201 aggtagtgtt ggtcagaccc agttttttgg cataaccatt catcaaacca ataaatgact
    19261 cctggctccc ggcaacgtaa tcagccagcg caatacaggc gtcattaccg gactggataa
    19321 tcacaccttt gttcaagtct gccaccgaaa cctgatcgcc cggtttgagg aacattaccg
    19381 atgaaccacg cagtgccgga tttcccgtcg cccaggcatc tttaccgacc gtcaccatat
    19441 cggtgagttt aatcttatcg gccttaagcg cctgcccaac cacatagctg gtcataattt
    19501 tagtcaggct cgcgggatcc agtttctcat ccgcattgcc ttctgccagc actttaccgc
    19561 tggcgtaatc cattaaaatc catgcacgcg catccacgct cggcgcttca acggtttgtt
    19621 ccgccgcgaa tgccgttggg gcaaaaagga ataaaaatgc agaacccgct gcaagaccac
    19681 gaaggagaga ggagtattgc gtcataaatg ccacccgagt atccattcaa aaaaaatacg
    19741 ttatatcgcc gtatgataag ttcggtgatt aataacgcac acacaaactt aaagaaaccg
    19801 taagttggta aagtttttaa agtttatgca atatcctgcc gcttcgctgc ttctggtgtc
    19861 atttttttgc cagctgttgc ttaaaagtta actttacttc accatcggag tcctgttcag
    19921 cctgtggctg cctgtcggaa aaggggtgag tatgattacg ctgtggggtc ggaataattc
    19981 aaccaacgta aaaaaagtat tgctgacgct cgaagaactg gaactacctt atgagcaaat
    20041 tctcgcgggc cgtgagtttg ggataaatca cgatgctgat tttctggcga tgaaccctaa
    20101 cgggctggtg ccgttgttgc gtgacgacga aagtgatctc attctttggg aatcaaacgc
    20161 cattgtccgc tatctggcgg cacagtacgg gcaaaaacgc ctgtggatcg actcaccggc
    20221 acgtcgtgcg gaagcagaaa aatggatgga ctgggcaaac cagacgctca gtaatgctca
    20281 tcgcgggatc ctgatgggat tagtcagaac accaccggaa gagcgcgatc aggccgccat
    20341 tgatgccagt tgcaaagagt gcgacgccct gtttgccctg ctcgatgcgg aactggcaaa
    20401 agtaaaatgg ttctcaggcg acgagtttgg tgtgggcgat atcgctatcg caccgttcat
    20461 ctacaatttg tttaacgttg gcctgacctg gacaccgcgt ccaaatctgc aacgctggta
    20521 tcagcaactc actgaacgcc ccgcagtgcg caaagtggtg atgattcccg ttagctaatt
    20581 gcgtgggcta actttaagta attccccact ggactcgtcg gtgagaacgt ataaataacc
    20641 gtcgggtcca gtgcgaacat cacgaattcg ctgccctctg tccgttaaaa tacggccatc
    20701 ttctgtcact ttgtcgccgt tgacgctcat cacaatgaca tctttatctt tcagcgcgcc
    20761 aataaataat ttttgctgcc actgggggaa tttgtcgctg ttatagaagg ccatgccgct
    20821 cacggcgggc gaatctttcc agtaaaaaac aggttgctcg gtcccggcga cgatctcccc
    20881 tttcgcttcc ggtatcttaa agcccgaata gttgattccc caggttgcca gcggccagcc
    20941 gtagtttttg cctttttgcg gaatattaat ttcatcacca ccgcgcgggc catgttcatt
    21001 cagccatagt gcattactcc acggattcat cgccattcct tgcggattac gaatgccata
    21061 agaccagatc tcggcgcgca caccagattc ctttataaaa ggattatcat ccgggatttc
    21121 tcccaggtcg gtcagacgca ccagtttgcc ctgtaattta tccagatcct gcgccgtcgg
    21181 gcgctgattg ttttcgccca gagcaataaa aagataacct ttaccgtcaa ataccatccg
    21241 cccgccaaaa tggttgccag tagacagttt tggcatctga cgaaagacgg tgcggaagtc
    21301 ggtcactttt gagagatcat cacttaagcg gccataaccc acagcagttc cggctttgcc
    21361 atcatcgcca acttcggaat aacttaacca gatgcggcgc gactgagcaa aatcaggcgc
    21421 taaaaccacg tccagcagac cgccctgccc gtgcgcccaa acgtccggca ctccggaaag
    21481 cggcgcagat aatccttttc ctgcttgcca gtgacgcaac tcgccgccgc gcagagtgat
    21541 taacataccg tgattatcgg gtaaaaaggc cagcgcccag ggatggtcga gtttgtcttg
    21601 cagtacttcg acatttaccg ttgcaggagc tgcccagaga gcggaagaaa gacaaataag
    21661 gggcacaagg aaaaaggatt gtcgatgcat aacgcctccg gattgactct ggcttaaagc
    21721 gtagtcagtg gaggagataa caaattcatt tttacaaaaa cttaaacatg aagggggaga
    21781 cgctttctcc cccttagttt tcaggccttc tcaagcatgg tgtgcttctg caggctctgg
    21841 atactcagcg ttaagctcat cagacaattt tcaagcttat cggcgttgac ggtaataaca
    21901 gtcgggcaat catggtgccc actcatcaaa catactgcgg ctgtcgctaa tgcttcttca
    21961 gcatgatgaa gagcactcca ctcttcctga tccagatgaa gattcaaccg cagcgattta
    22021 tcgtgcagtt cgcgattcag tttaaaaaag ttatctcgta gatgattgct ttcgctgacg
    22081 gacatgtatc cttttgcctt cctcagttgc acgtatgcgg cgaggtcaac gcgtgcgtcg
    22141 atcaaccggt tcagcagatc gattcgctgt ctgtcaacga acatacttcg atcctcctct
    22201 ttccagcctt gttgcaatta aatagtatgg ctgttttttg agcaaaatgc tgtccaggat
    22261 caattcttca cgccgaatta acaaaattta catttccttc aactccgcca gcgaatgaga
    22321 ttgcgataca aattttatcc agaactctgt agaatccctg ccctgacctt tcctgtaact
    22381 gaatattttt taagctatga gcaaagtaac tccccagccg aaaatcggct ttgtttccct
    22441 tggctgtccg aaaaaccttg tcgattcaga gcgtattctc accgaactac gcactgaagg
    22501 ttatgacgtg gtaccgagct atgatgatgc ggacatggtg atcgtcaaca cctgcggctt
    22561 tattgacagc gcggtacaag aatcactgga agccattggt gaagcgttga atgaaaacgg
    22621 caaggtaatt gtgaccggtt gtctgggggc aaaagaagat cagatccgcg aagtccaccc
    22681 gaaagtgctg gaaatcactg ggcctcatag ctatgagcag gttctggagc acgttcatca
    22741 ctacgtacca aaaccgaaac acaacccatt tctgagcctg gtgccagaac aaggtgtgaa
    22801 actgacgccg cgtcattatg cctatctgaa aatttctgaa ggctgtaatc accgctgcac
    22861 cttctgcatt attccgtcta tgcgcggcga tctggtgagc cgtccgattg gcgaagtgtt
    22921 aagtgaagcg aaacgtctgg ttgatgcggg cgttaaagaa attctggtga tctcgcagga
    22981 tacttccgcg tatggcgtcg atgttaaaca tcgtactggc ttccacaacg gcgagccggt
    23041 aaaaaccagc atggtcagcc tgtgcgaaca gttatcgaaa ctggggatct ggacacgtct
    23101 gcactacgtt tacccttatc cgcatgtgga cgacgtcatc ccactgatgg cagagggcaa
    23161 aatcctgccg tatctggaca ttccgttgca gcacgccagc ccgcgcattc tcaaactgat
    23221 gaagcgtccg ggttctgtag atcgccaact ggcgcgcatc aaacagtggc gcgaaatctg
    23281 cccggaactg accctacgct caacctttat tgttggcttc cctggcgaga cggaagaaga
    23341 tttccagatg ctactcgact tcctgaaaga agcacgtctg gatcgcgttg gctgctttaa
    23401 atacagcccg gttgaaggtg cagacgccaa tgccctgcct gaccaggttc cggaagaagt
    23461 gaaagaagaa cgctggaacc gtttcatgca gttgcagcag caaatttccg ccgagcgcct
    23521 gcaagagaaa gtgggccgtg aaattctggt gattatcgac gaagtggacg aagaaggcgc
    23581 gattggtcgc agcatggcag atgcaccgga aatcgacggc gcggtttatc tcaatggtga
    23641 aaccaacgtt aagccgggtg atatcctgcg agtgaaagtc gagcacgccg atgagtacga
    23701 tttgtggggt agccgggttt aattaattca ggccgggcaa aatcatttgt ccggcctgcg
    23761 aataatgatt actgccagct aatctgcgcc ctgccgtttt tcttactctt atacagccgc
    23821 tcatccgcca gcattttcaa acgttcaacg gtagtacatt catttgaatg cgctacaccg
    23881 atactggtgc ttaaattaat gcttcccacc aggaaatgaa cgatttcttt tagaatcgat
    23941 agcaactctt ccagcgtgcc gccagtataa atcaccgcaa attcgtcacc tccgaagcgg
    24001 taaactttac ctttatctac cgggatctgt gatttaagga ttttcaccac tttcatcaac
    24061 acttcatcgc ccagcaaatg accgaaggtg tcgttaatgt ttttgaattt atcgacgtca
    24121 aacaggcaca aataaccgcc tttttccggc agcgctttta aatcttcgtc aaaagatttt
    24181 ctgcccatcg ctttggtcag atcgtccagg aatgccgagt tatacatact gtagagtttt
    24241 ttgacatcgg cagccgaaaa gaaaagcccg ccatccagta cgcgttcaaa atggttatta
    24301 ataatggctt ctttctccac aataccacga ttaatcactc tgcgtaccag atagaggcaa
    24361 ctggcacccg atatcactgc caccaatgcg gcaaagaaaa taatcaatgt acatacctga
    24421 aaataaacgt cttttttata agcaaatacg tacaacccgg tcggcaaata ggagaagtgc
    24481 tccattgctt ccgaaacggt gaagatggcg tttttagcgt tatattcatc accggtcacc
    24541 gatttaccgt tcttgaacag ggagaacatc aggttctcgc tatccatcgc cagcgatttt
    24601 gacagtaaag aaaaacgttc cagaccaagc aaaaatatca gactacgatt gtgagttacc
    24661 ggatagaggt aatagacgaa agttttacca tcaaaagaga taaaccctgt atttttgaca
    24721 tgtcgatact taacaacctg cataatatct gcacaataat ttttctcgaa ctcctttacc
    24781 tgaatacatt catcactgga aatcagataa ggaccgacat cgtacaaata agagggtgaa
    24841 accgagtcgg cgtaagctgt gcgtaagcca accaacagct gttcctccgt aggatttagc
    24901 gtgttgatga gatcaattgc cacactacgc cgatattcag caatatttgc caccgcaaaa
    24961 gcagtatcaa taacggactg tttcaggcca tacacccgtt cctttaccat ttgagtgtag
    25021 atcccgcagg caactgctga aatcatgata aaaatcagca gactgactgt aagtacgaat
    25081 ttattgattc tggacatacg taaactactc ttttactaat tttccacttt tatcccaggc
    25141 ggagaatggc atgggtttct ggtacagata cccctgagcc tgaacacatc ctaatttgat
    25201 taattcacgc gcaacgtctt cgctctctac gccttccgca atcaccggga tccggttata
    25261 gcgtgccaga tcgaaaataa tgcgcacaat ttttcctgca gtaccgaaaa tatcgccggt
    25321 aaattgcgaa tccaccttta tactgtctgg attaatttca taaaaacgtt tcaggtttga
    25381 caaaccggtg ccaaagtcat caatcgatac ccgtacccct tgtttacgca gttgttcaat
    25441 attgagatag aaatgttcat taattcgctc gatcacggtt tcggtgatct caacacaaag
    25501 gctgttcgcc ttcaggccat tttctttcat gatggtagta aaacgctcat gaaagtgtgg
    25561 ttcgtttaac tgccagggcg aaacattaat atgcagtaag aaatcatcct tcagcgacgc
    25621 acgctgacta aaatgtctga attcacccat cgcggtttta ataatttgat agcccagctc
    25681 attgataaac ccgatatctt cagcaatggt aatgaacttc agcggtgaaa taatcccccg
    25741 ctccgcagac acccaacggc acaacgcctc cgcgcccaaa atggtgcggg tgttaatatc
    25801 aacaatcggt tgatagacca ggaatagttc gccaatttcc attgcatggc gaatattacg
    25861 cgcaatccag atattctctt ctatttcgtt gagtttcgca tcatcacaga aaataaactc
    25921 tgagaacata tggttgcgga tactggaaat cgccagtttc agcttttctg cgtaactcca
    25981 ggcgttttcc ccctgcaaag gttcacaaat agcagactta tggatgcgca gattgcggta
    26041 gccaaaactg ctgtcaaaat cgtttaccgt cgctatctta cgggtaaacg tctgtacatt
    26101 ttcccggcac aggagtaaat agagatcggc attaaatgtc acgatatcgc agcattgcgc
    26161 atatttctcg gcaattcgct gacacaattt cctgacgatt ttattgcgtt catcaacacc
    26221 aaacaccgct tccacagctt ccagaccaca cagtttgatg atccccacag ccagatactg
    26281 attattgttg gactcaataa tcgccatatt tttaataaaa ccgctttcct gatcgtaaga
    26341 gatcagcgag agcaatttat ttgcccgttc aatttcagca tctttcattt ccgcgatcga
    26401 atttttgatg ctgataatct ctttcgcatc gccttcacga atcttcaggg cctcgatttg
    26461 atccgtagag tcaggcaaaa acgccagcgt attggccaaa tctgtcattt cagaaaattg
    26521 ccgacggaac actcgagtgg tcattaaata aaccaccagc aatacgataa taatgaaagc
    26581 aaacggcaga taaaaaagtt tatctgatgc gttcaataaa ttaccaccag gataataaaa
    26641 atagatttcg aataatgcgt tgtttggcac cg
//
LOCUS       DADSYM010000062        26579 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2415
            SAMN08039579-rid11723173.denovo.062, whole genome shotgun sequence.
ACCESSION   DADSYM010000062 DADSYM010000000
VERSION     DADSYM010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039579
            Sequence Read Archive: SRR6362533
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 26579)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26579)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980162.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 03:39:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,484
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 4,347
            CDSs (with protein)               :: 4,347
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 43 of 85
            Pseudo Genes (incomplete)         :: 37 of 85
            Pseudo Genes (internal stop)      :: 19 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26579
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039579-rid11723173.denovo.062"
                     /strain="LGE2415"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8302 N 95.9628 W"
                     /collection_date="2015-08-24"
     gene            83..1552
                     /gene="gspA"
                     /locus_tag="J1Y83_002148"
     CDS             83..1552
                     /gene="gspA"
                     /locus_tag="J1Y83_002148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspA"
                     /protein_id="HBA7630295.1"
                     /translation="MSTRREVILSWLREKRQTWRLCYLLGEAGSGKTWLAQQLQKDKH
                     RRVITLSLVVSWQGKAAWIVTDDNAAEQGCRDSAWTRDEMAGQLLHALHRTDSRCPLI
                     IIENAHLNHRRILDDLQRAISLIPDGQFLLIGRPDRKVERDFKKQGIELVSTGRLMEH
                     ELKASILEGQNIDQPDLLLTARVLKRIALLCRGDRRKLALAGETISLLQQAEQTRVFT
                     AKQWRMIYRVLGDNRPRKMQLAVVMSGTIIALTCGWLLLSSFTAPLPVPAWLIPVTPV
                     AKQDMTKDIAHVVMRDSEALSVLYGVWGYEVPADSAWCDQAVRAGLVCKSGNASLQTL
                     VDQNLPWIASLKVGDKKLPVVVVRVGDATVDVLVGQQTWTLTHKWFELVWTGDYLLLW
                     KMSPEGESTITRDSSEEEILWLETMLNRALHISTESSAEWRPLLVEKIKQFQKSHHLK
                     TDGVVGFSTLVHLWQVAGESAYLYRDEANISPETTVKGK"
     gene            1554..1973
                     /gene="gspB"
                     /locus_tag="J1Y83_002149"
     CDS             1554..1973
                     /gene="gspB"
                     /locus_tag="J1Y83_002149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative general secretion pathway protein GspB"
                     /protein_id="HBA7630296.1"
                     /translation="MFEFYIAAREQKETGHPGIFSRQKHSTIIYVICLLLICLWFAGM
                     VLVGGYARQLWALWIVKAEVTVDAETPAFKQSTQHYFFKKQPLPVVESVEEEDEPGVA
                     VENAPSSSEDEENTVEESDEKAGLRERVKNALNELER"
     gene            2211..2522
                     /gene="rpsJ"
                     /locus_tag="J1Y83_002150"
     CDS             2211..2522
                     /gene="rpsJ"
                     /locus_tag="J1Y83_002150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014268624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HBA7630297.1"
                     /translation="MQNQRIRIRLKAFDHRLIDQATAEIVETAKRTGAQVRGPIPLPT
                     RKERFTVLISPHVNKDARDQYEIRTHLRLVDIVEPTEKTVDALMRLDLAAGVDVQISL
                     G"
     gene            2555..3184
                     /gene="rplC"
                     /locus_tag="J1Y83_002151"
     CDS             2555..3184
                     /gene="rplC"
                     /locus_tag="J1Y83_002151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000579825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HBA7630298.1"
                     /translation="MIGLVGKKVGMTRIFTEDGVSIPVTVIEVEANRVTQVKDLANDG
                     YRAIQVTTGAKKANRVTKPEAGHFAKAGVEAGRGLWEFRLAEGEEFTVGQSISVELFA
                     DVKKVDVTGTSKGKGFAGTVKRWNFRTQDATHGNSLSHRVPGSIGQNQTPGKVFKGKK
                     MAGQMGNERVTVQSLDVVRVDAERNLLLVKGAVPGATGSDLIVKPAVKA"
     gene            3195..3800
                     /gene="rplD"
                     /locus_tag="J1Y83_002152"
     CDS             3195..3800
                     /gene="rplD"
                     /locus_tag="J1Y83_002152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010304939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HBA7630299.1"
                     /translation="MELVLKDAQSALTVSETTFGRDFNEALVHQVVVAYAAGARQGTR
                     AQKTRAEVTGSGKKPWRQKGTGRARSGSIKSPIWRSGGVTFAARPQDHSQKVNKKMYR
                     GALKSILSELVRQDRLIVVEKFSVEAPKTKLLAQKLKDMALEDVLIITGELDENLFLA
                     ARNLHKVDVRDATGIDPVSLIAFDKVVMTADAVKQVEEMLA"
     gene            3797..4099
                     /gene="rplW"
                     /locus_tag="J1Y83_002153"
     CDS             3797..4099
                     /gene="rplW"
                     /locus_tag="J1Y83_002153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007891652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HBA7630300.1"
                     /translation="MIREERLLKVLRAPHVSEKASTAMEKSNTIVLKVAKDATKAEIK
                     AAVQKLFEVEVEVVNTLVVKGKVKRHGQRIGRRSDWKKAYVTLKEGQNLDFVGGAE"
     gene            4117..4938
                     /gene="rplB"
                     /locus_tag="J1Y83_002154"
     CDS             4117..4938
                     /gene="rplB"
                     /locus_tag="J1Y83_002154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HBA7630301.1"
                     /translation="MAVVKCKPTSPGRRHVVKVVNPELHKGKPFAPLLEKNSKSGGRN
                     NNGRITTRHIGGGHKQAYRIVDFKRNKDGIPAVVERLEYDPNRSANIALVLYKDGERR
                     YILAPKGLKAGDQIQSGVDAAIKPGNTLPMRNIPVGSTVHNVEMKPGKGGQLARSAGT
                     YVQIVARDGAYVTLRLRSGEMRKVEADCRATLGEVGNAEHMLRVLGKAGAARWRGVRP
                     TVRGTAMNPVDHPHGGGEGRNFGKHPVTPWGVQTKGKKTRSNKRTDKFIVRRRSK"
     gene            4955..5233
                     /gene="rpsS"
                     /locus_tag="J1Y83_002155"
     CDS             4955..5233
                     /gene="rpsS"
                     /locus_tag="J1Y83_002155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HBA7630302.1"
                     /translation="MPRSLKKGPFIDLHLLKKVEKAVESGDKKPLRTWSRRSTIFPNM
                     IGLTIAVHNGRQHVPVFVTDEMVGHKLGEFAPTRTYRGHAADKKAKKK"
     gene            5248..5580
                     /gene="rplV"
                     /locus_tag="J1Y83_002156"
     CDS             5248..5580
                     /gene="rplV"
                     /locus_tag="J1Y83_002156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000447526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HBA7630303.1"
                     /translation="METIAKHRHARSSAQKVRLVADLIRGKKVSQALDILTYTNKKAA
                     VLVKKVLESAIANAEHNDGADIDDLKVTKIFVDEGPSMKRIMPRAKGRADRILKRTSH
                     ITVVVSDR"
     gene            5598..6299
                     /gene="rpsC"
                     /locus_tag="J1Y83_002157"
     CDS             5598..6299
                     /gene="rpsC"
                     /locus_tag="J1Y83_002157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000529946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HBA7630304.1"
                     /translation="MGQKVHPNGIRLGIVKPWNSTWFANTKEFADNLDSDFKVRQYLT
                     KELAKASVSRIVIERPAKSIRVTIHTARPGIVIGKKGEDVEKLRKVVADIAGVPAQIN
                     IAEVRKPELDAKLVADSITSQLERRVMFRRAMKRAVQNAMRLGAKGIKVEVSGRLGGA
                     EIARTEWYREGRVPLHTLRADIDYNTSEAHTTYGVIGVKVWIFKGEILGGMAAVEQPE
                     KPAAQPKKQQRKGRK"
     gene            6312..6722
                     /gene="rplP"
                     /locus_tag="J1Y83_002158"
     CDS             6312..6722
                     /gene="rplP"
                     /locus_tag="J1Y83_002158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017805229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HBA7630305.1"
                     /translation="MLQPKRTKFRKMHKGRNRGLAQGTDVSFGSFGLKAVGRGRLTAR
                     QIEAARRAMTRAVKRQGKIWIRVFPDKPITEKPLAVRMGKGKGNVEYWVALIQPGKVL
                     YEMDGVPEELAREAFKLAAAKLPIKTTFVTKTVM"
     gene            6722..6913
                     /gene="rpmC"
                     /locus_tag="J1Y83_002159"
     CDS             6722..6913
                     /gene="rpmC"
                     /locus_tag="J1Y83_002159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000644743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HBA7630306.1"
                     /translation="MKAKELREKSVEELNTELLNLLREQFNLRMQAASGQLQQSHLLK
                     QVRRDVARVKTLLNEKAGA"
     gene            6913..7167
                     /gene="rpsQ"
                     /locus_tag="J1Y83_002160"
     CDS             6913..7167
                     /gene="rpsQ"
                     /locus_tag="J1Y83_002160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006817275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HBA7630307.1"
                     /translation="MTDKIRTLQGRVVSDKMEKSIVVAIERFVKHPIYGKFIKRTTKL
                     HVHDENNECGIGDVVEIRECRPLSKTKSWTLVRVVEKAVL"
     gene            7332..7703
                     /gene="rplN"
                     /locus_tag="J1Y83_002161"
     CDS             7332..7703
                     /gene="rplN"
                     /locus_tag="J1Y83_002161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013975775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HBA7630308.1"
                     /translation="MIQEQTMLNVADNSGARRVMCIKVLGGSHRRYAGVGDIIKITIK
                     EAIPRGKVKKGDVLKAVVVRTKKGVRRPDGSVIRFDGNACVLLNNNSEQPIGTRIFGP
                     VTRELRSEKFMKIISLAPEVL"
     gene            7714..8028
                     /gene="rplX"
                     /locus_tag="J1Y83_002162"
     CDS             7714..8028
                     /gene="rplX"
                     /locus_tag="J1Y83_002162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000729185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HBA7630309.1"
                     /translation="MAAKIRRDDEVIVLTGKDKGKRGKVKNVLSSGKVIVEGINLVKK
                     HQKPVPALNQPGGIVEKEAAIQVSNVAIFNATTGKADRVGFRFEDGKKVRFFKSNSET
                     IK"
     gene            8043..8582
                     /gene="rplE"
                     /locus_tag="J1Y83_002163"
     CDS             8043..8582
                     /gene="rplE"
                     /locus_tag="J1Y83_002163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HBA7630310.1"
                     /translation="MAKLHDYYKDEVVKKLMTEFNYNSVMQVPRVEKITLNMGVGEAI
                     ADKKLLDNAAADLAAISGQKPLITKARKSVAGFKIRQGYPIGCKVTLRGERMWEFFER
                     LITIAVPRIRDFRGLSAKSFDGRGNYSMGVREQIIFPEIDYDKVDRVRGLDITITTTA
                     KSDEEGRALLAAFDFPFRK"
     gene            8597..8902
                     /gene="rpsN"
                     /locus_tag="J1Y83_002164"
     CDS             8597..8902
                     /gene="rpsN"
                     /locus_tag="J1Y83_002164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005599304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HBA7630311.1"
                     /translation="MAKQSMKAREVKRVALADKYFAKRAELKAIISDVNASDEDRWNA
                     VLKLQTLPRDSSPSRQRNRCRQTGRPHGFLRKFGLSRIKVREAAMRGEIPGLKKASW"
     gene            8936..9328
                     /gene="rpsH"
                     /locus_tag="J1Y83_002165"
     CDS             8936..9328
                     /gene="rpsH"
                     /locus_tag="J1Y83_002165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HBA7630312.1"
                     /translation="MSMQDPIADMLTRIRNGQAANKAAVTMPSSKLKVAIANVLKEEG
                     FIEDFKVEGDTKPELELTLKYFQGKAVVESIQRVSRPGLRIYKRKDELPKVMAGLGIA
                     VVSTSKGVMTDRAARQAGLGGEIICYVA"
     gene            9341..9874
                     /gene="rplF"
                     /locus_tag="J1Y83_002166"
     CDS             9341..9874
                     /gene="rplF"
                     /locus_tag="J1Y83_002166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003031129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HBA7630313.1"
                     /translation="MSRVAKAPVVVPAGVDVKINGQVITIKGKNGELTRTLNDAVEVK
                     HADNTLTFGPRDGYADGWAQAGTARALLNSMVIGVTEGFTKKLQLVGVGYRAAVKGNV
                     INLSLGFSHPVDHQLPAGITAECPTQTEIVLKGADKQVIGQVAADLRAYRRPEPYKGK
                     GVRYADEVVRTKEAKKK"
     gene            9884..10237
                     /gene="rplR"
                     /locus_tag="J1Y83_002167"
     CDS             9884..10237
                     /gene="rplR"
                     /locus_tag="J1Y83_002167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005970258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HBA7630314.1"
                     /translation="MDKKSARIRRATRARRKLQELGATRLVVHRTPRHIYAQVIAPNG
                     SEVLVAASTVEKAIAEQLKYTGNKDAAAAVGKAVAERALEKGIKDVSFDRSGFQYHGR
                     VQALADAAREAGLQF"
     gene            10252..10755
                     /gene="rpsE"
                     /locus_tag="J1Y83_002168"
     CDS             10252..10755
                     /gene="rpsE"
                     /locus_tag="J1Y83_002168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004106339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HBA7630315.1"
                     /translation="MAHIEKQAGELQEKLIAVNRVSKTVKGGRIFSFTALTVVGDGNG
                     RVGFGYGKAREVPAAIQKAMEKARRNMINVALNNGTLQHPVKGVHTGSRVFMQPASEG
                     TGIIAGGAMRAVLEVAGVHNVLAKAYGSTNPINVVRATIDGLENMNSPEMVAAKRGKS
                     VEEILGK"
     gene            10759..10938
                     /gene="rpmD"
                     /locus_tag="J1Y83_002169"
     CDS             10759..10938
                     /gene="rpmD"
                     /locus_tag="J1Y83_002169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012147215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HBA7630316.1"
                     /translation="MAKTIKITQTRSAIGRLPKHKATLLGLGLRRIGHTVEREDTPAI
                     RGMINAVSFMVKVEE"
     gene            10942..11376
                     /gene="rplO"
                     /locus_tag="J1Y83_002170"
     CDS             10942..11376
                     /gene="rplO"
                     /locus_tag="J1Y83_002170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011095501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HBA7630317.1"
                     /translation="MRLNTLSPAEGSKKAGKRLGRGIGSGLGKTGGRGHKGQKSRSGG
                     GVRRGFEGGQMPLYRRLPKFGFTSRKAAITAEVRLSDLAKVEGGVVDLNTLKAANIIG
                     IQIEFAKVILAGEVTTPVTVRGLRVTKGARAAIEAAGGKIEE"
     gene            11384..12715
                     /gene="secY"
                     /locus_tag="J1Y83_002171"
     CDS             11384..12715
                     /gene="secY"
                     /locus_tag="J1Y83_002171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004868351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HBA7630318.1"
                     /translation="MAKQPGLDFQSAKGGLGELKRRLLFVIGALIVFRIGSFIPIPGI
                     DAAVLAKLLEQQRGTIIEMFNMFSGGALSRASIFALGIMPYISASIIIQLLTVVHPTL
                     AEIKKEGESGRRKISQYTRYGTLVLAIFQSIGIATGLPNMPGMQGLVINPGFAFYFTA
                     VVSLVTGTMFLMWLGEQITERGIGNGISIIIFAGIVAGLPPAIAHTIEQARQGDLHFL
                     VLLLVAVLVFAVTFFVVFVERGQRRIVVNYAKRQQGRRVYAAQSTHLPLKVNMAGVIP
                     AIFASSIILFPATIASWFGGGTGWNWLTTISLYLQPGQPLYVLLYASAIIFFCFFYTA
                     LVFNPRETADNLKKSGAFVPGIRPGEQTAKYIDKVMTRLTLVGALYITFICLIPEFMR
                     DAMKVPFYFGGTSLLIVVVVIMDFMAQVQTLMMSSQYESALKKANLKGYGR"
     gene            12747..12863
                     /gene="rpmJ"
                     /locus_tag="J1Y83_002172"
     CDS             12747..12863
                     /gene="rpmJ"
                     /locus_tag="J1Y83_002172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020322760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HBA7630319.1"
                     /translation="MKVRASVKKLCRNCKIVKRDGVIRVICSAEPKHKQRQG"
     gene            13010..13366
                     /gene="rpsM"
                     /locus_tag="J1Y83_002173"
     CDS             13010..13366
                     /gene="rpsM"
                     /locus_tag="J1Y83_002173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018356918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HBA7630320.1"
                     /translation="MARIAGINIPDHKHAVIALTSIYGVGKTRSKAILAAAGIAEDVK
                     ISELSEGQIDTLRDEVAKFVVEGDLRREISMSIKRLMDLGCYRGLRHRRGLPVRGQRT
                     KTNARTRKGPRKPIKK"
     gene            13383..13772
                     /gene="rpsK"
                     /locus_tag="J1Y83_002174"
     CDS             13383..13772
                     /gene="rpsK"
                     /locus_tag="J1Y83_002174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HBA7630321.1"
                     /translation="MAKAPIRARKRVRKQVSDGVAHIHASFNNTIVTITDRQGNALGW
                     ATAGGSGFRGSRKSTPFAAQVAAERCADAVKEYGIKNLEVMVKGPGPGRESTIRALNA
                     AGFRITNITDVTPIPHNGCRPPKKRRV"
     gene            13806..14426
                     /gene="rpsD"
                     /locus_tag="J1Y83_002175"
     CDS             13806..14426
                     /gene="rpsD"
                     /locus_tag="J1Y83_002175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004410410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HBA7630322.1"
                     /translation="MARYLGPKLKLSRREGTDLFLKSGVRAIDTKCKIEQAPGQHGAR
                     KPRLSDYGVQLREKQKVRRIYGVLERQFRNYYKEAARLKGNTGENLLALLEGRLDNVV
                     YRMGFGATRAEARQLVSHKAIMVNGRVVNIASYQVSPNDVVSIREKAKKQSRVKAALE
                     LAEQREKPTWLEVDAGKMEGTFKRKPERSDLSADINEHLIVELYSK"
     gene            14452..15441
                     /gene="rpoA"
                     /locus_tag="J1Y83_002176"
     CDS             14452..15441
                     /gene="rpoA"
                     /locus_tag="J1Y83_002176"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005970247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HBA7630323.1"
                     /translation="MQGSVTEFLKPRLVDIEQVSSTHAKVTLEPLERGFGHTLGNALR
                     RILLSSMPGCAVTEVEIDGVLHEYSTKEGVQEDILEILLNLKGLAVRVQGKDEVILTL
                     NKSGIGPVTAADITHDGDVEIVKPQHVICHLTDENASISMRIKVQRGRGYVPASTRIH
                     SEEDERPIGRLLVDACYSPVERIAYNVEAARVEQRTDLDKLVIEMETNGTIDPEEAIR
                     RAATILAEQLEAFVDLRDVRQPEVKEEKPEFDPILLRPVDDLELTVRSANCLKAEAIH
                     YIGDLVQRTEVELLKTPNLGKKSLTEIKDVLASRGLSLGMRLENWPPASIADE"
     gene            15482..15865
                     /gene="rplQ"
                     /locus_tag="J1Y83_002177"
     CDS             15482..15865
                     /gene="rplQ"
                     /locus_tag="J1Y83_002177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HBA7630324.1"
                     /translation="MRHRKSGRQLNRNSSHRQAMFRNMAGSLVRHEIIKTTLPKAKEL
                     RRVVEPLITLAKTDSVANRRLAFARTRDNEIVAKLFNELGPRFASRAGGYTRILKCGF
                     RAGDNAPMAYIELVDRSEKAEAAAE"
     gene            15972..16340
                     /locus_tag="J1Y83_002178"
     CDS             15972..16340
                     /locus_tag="J1Y83_002178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1992 domain-containing protein"
                     /protein_id="HBA7630325.1"
                     /translation="MWLLDQWAERHIAEAQAKGEFDNLVGSGEPLILDDDSHVPPELR
                     AGYRLLKNAGCLPPELEHRREAIQLLDILKGIRHDDPQYQEVSRRLSLLELKLRQAGL
                     STDFLRGDYADKLLNKINDN"
     gene            16351..16776
                     /gene="zntR"
                     /locus_tag="J1Y83_002179"
     CDS             16351..16776
                     /gene="zntR"
                     /locus_tag="J1Y83_002179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn(2+)-responsive transcriptional regulator"
                     /protein_id="HBA7630326.1"
                     /translation="MYRIGELAKMAEVTPDTIRYYEKQQMMEHEVRTEGGFRLYTESD
                     LQRLKFIRHARQLGFSLESIRELLSIRIDPEHHTCQESKGIVQERLQEVEARIAELQS
                     MQRSLQRLNDTCCGTAHSSVYCSILEALEQGASGVKSGC"
     gene            16832..17050
                     /locus_tag="J1Y83_002180"
     CDS             16832..17050
                     /locus_tag="J1Y83_002180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alternative ribosome-rescue factor A"
                     /protein_id="HBA7630327.1"
                     /translation="MSRYQHTKGQIKDNAIEALLHDPLFRQRVEKNKKGKGSYMRKGK
                     HGNRGNWEASGKKVNHFFTTGLLLSDAC"
     gene            complement(17047..17460)
                     /gene="mscL"
                     /locus_tag="J1Y83_002181"
     CDS             complement(17047..17460)
                     /gene="mscL"
                     /locus_tag="J1Y83_002181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="large-conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HBA7630328.1"
                     /translation="MSIIKEFREFAMRGNVVDLAVGVIIGAAFGKIVSSLVADIIMPP
                     LGLLIGGIDFKQFAVTLRDAQGDIPAVVMHYGVFIQNVFDFLIVAFAIFMAIKLINKL
                     NRKKEEPAAATPAPTKEEVLLTEIRDLLKEQNNRS"
     gene            complement(17590..18966)
                     /gene="trkA"
                     /locus_tag="J1Y83_002182"
     CDS             complement(17590..18966)
                     /gene="trkA"
                     /locus_tag="J1Y83_002182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004125888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkA"
                     /protein_id="HBA7630329.1"
                     /translation="MKIIILGAGQVGGTLAENLVGENNDITVVDTNGERLRTLQDKFD
                     LRVVQGHGSHPRVLREAGADDADMLVAVTSSDETNMVACQVAYSLFNTPNRIARIRSP
                     DYVRDADKLFHSDAVPIDHLIAPEQLVIDNIYRLIEYPGALQVVNFAEGKVSLAVVKA
                     YYGGPLIGNALSTMREHMPHIDTRVAAIFRHDRPIRPQGSTIVEAGDEVFFIAASQHI
                     RAVMSELQRLEKPYKRIMLVGGGNIGAGLARRLEKDYSVKLIERNQQRAAELAEKLQN
                     TIVFFGDASDQELLAEEHIDQVDLFIAVTNDDEANIMSAMLAKRMGAKKVMVLIQRRA
                     YVDLVQGSVIDIAISPQQATISALLSHVRKADIVGVSSLRRGVAEAIEAVAHGDESTS
                     RVVGRVIDEIKLPPGTIIGAVVRGNDVMIANDNLRIEQGDHVIMFLTDKKFITDVERL
                     FQPSPFFL"
     gene            complement(18988..20277)
                     /gene="rsmB"
                     /locus_tag="J1Y83_002183"
     CDS             complement(18988..20277)
                     /gene="rsmB"
                     /locus_tag="J1Y83_002183"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HBA7630330.1"
                     /translation="MKKQRNLRSMAAQAIEQVVEQGQSLSNILPPLQQKVSDKDKALL
                     QELCFGVLRTLSQLDWLINKLMARPMTGKQRTVHYLIMVGLYQLLYTRIPPHAALAET
                     VEGAVAIKRPQLKGLINGVLRQFQRQQDELLAEFNASDARYLHPSWLLKRLQKAYPEQ
                     WQSIVEANNQRPPMWLRVNRTHHSRDSWLALLDEAGMKGFPHADYPDAVRLETPAPVH
                     ALPGFEDGWVTVQDASAQGCMTWLAPQNGEHILDLCAAPGGKTTHILEVAPEAQVVAV
                     DIDEQRLSRVYDNLKRLGMKATVKQGDGRYPSQWCGEQQFDRILLDAPCSATGVIRRH
                     PDIKWLRRDRDIPELAQLQSEILDAIWPHLKSGGTLVYATCSVLPEENSLQIKAFLQR
                     TADAELCETGTPEQPGKQNLPGAEEGDGFFYAKLIKK"
     gene            complement(20329..21276)
                     /gene="fmt"
                     /locus_tag="J1Y83_002184"
     CDS             complement(20329..21276)
                     /gene="fmt"
                     /locus_tag="J1Y83_002184"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HBA7630331.1"
                     /translation="MSESLRIIFAGTPDFAARHLDALLSSGHNIVGVFTQPDRPAGRG
                     KKLMPSPVKVLAEDKGLPVFQPVSLRPQENQQLVADLQADVMVVVAYGLILPKAVLEM
                     PRLGCINVHGSLLPRWRGAAPIQRSLWAGDTETGVTIMQMDIGLDTGDMLYKLSCPIT
                     AEDTSGTLYDKLAELGPQGLITTLKQLADGTAKPEVQDETLVTYAEKLSKEEARIDWS
                     LSAAQLERCIRAFNPWPMSWLEIEGQPVKVWKASVIDTTTKAAPGTILEANKQGIQVA
                     TGDGILNLLSMQPAGKKAMSVQDLLNSRREWFVPGNRLA"
     gene            complement(21291..21800)
                     /gene="def"
                     /locus_tag="J1Y83_002185"
     CDS             complement(21291..21800)
                     /gene="def"
                     /locus_tag="J1Y83_002185"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HBA7630332.1"
                     /translation="MSVLQVLHIPDERLRKVAKPVEEVNAEIQRIVDDMFETMYAEEG
                     IGLAATQVDIHQRIIVIDVSENRDERLVLINPELLEKSGETGIEEGCLSIPEQRALVP
                     RAEKVKIRALDRDGKPFELEADGLLAICIQHEMDHLVGKLFMDYLSPLKQQRIRQKVE
                     KLDRLKARA"
     gene            21930..23054
                     /gene="dprA"
                     /locus_tag="J1Y83_002186"
     CDS             21930..23054
                     /gene="dprA"
                     /locus_tag="J1Y83_002186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HBA7630333.1"
                     /translation="MVDTEIWLRLISISSLYGDDMVRIAHWLAKQSHIDAVVLQQTGL
                     TLRQAQRFLSFPRKSIESSLCWLEQPNHHLIPADSEFYPPQLLATTDYPGALFVEGEL
                     HALHSFQLAVVGSRAHSWYGERWGRLFCETLATRGVTITSGLARGIDGVAHKAALQVN
                     GVSIAVLGNGLNTIHPRRHAPLAASLLEQGGALVSEFPLDVPPLAYNFPRRNRIISGL
                     SKGVLVVEAALRSGSLVTARCALEQGREVFALPGPIGNPGSEGPHWLIKQGAILVTEP
                     EEILENLQFGLHWLPDAPENSFYSPDQEDVALPFPELLANVGDEVTPVDVVAERAGQP
                     VPEVVTQLLELELAGWIAAVPGGYVRLRRACHVRRTNVFV"
     gene            23026..23499
                     /locus_tag="J1Y83_002187"
     CDS             23026..23499
                     /locus_tag="J1Y83_002187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF494 domain-containing protein"
                     /protein_id="HBA7630334.1"
                     /translation="MFDVLMYLFETYIHTEAELRVDQDKLEQDLTDAGFDREDIYNAL
                     LWLEKLADYQEGLAEPMQLASDPLSMRIYTPEECERLDASCRGFLLFLEQIQVLNLET
                     REMVIERVLALDTAEFDLEDLKWVILMVLFNIPGCENAYQQVEELLFEVNEGMLH"
     gene            23528..24070
                     /locus_tag="J1Y83_002188"
     CDS             23528..24070
                     /locus_tag="J1Y83_002188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA7630335.1"
                     /translation="MAKSALFTVRNNESCPKCGAELVIRSGKHGPFLGCSQYPACDYV
                     RPLKSSADGHIVKVLEGQVCPACGANLVLRQGRFGMFIGCSNYPECEHTELIDKPDET
                     AITCPQCRTGHLVQRRSRYGKTFHSCDRYPECQFAINFKPIAGECPECHYPLLIEKKT
                     AQGVKHFCASKQCGKPISAE"
     gene            24075..24647
                     /gene="tsaC"
                     /locus_tag="J1Y83_002189"
     CDS             24075..24647
                     /gene="tsaC"
                     /locus_tag="J1Y83_002189"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase type 1
                     TsaC"
                     /protein_id="HBA7630336.1"
                     /translation="MNNNLQGDAIAAAIDVLNEERVIAYPTEAVFGVGCDPDSETAVM
                     RLLELKRRPVDKGLILIAANYEQLKPYIDDTMLTDAQRETIFSRWPGPVTFVFPAPAT
                     TPRWLTGRFDSLAVRVTDHPLVVALCQAYGKPLVSTSANLSGLPPCRTVDEVRAQFGA
                     AFPVVPGETGGRLNPSEIRDALTGELFRQG"
     gene            24652..25470
                     /gene="aroE"
                     /locus_tag="J1Y83_002190"
     CDS             24652..25470
                     /gene="aroE"
                     /locus_tag="J1Y83_002190"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HBA7630337.1"
                     /translation="METYAVFGNPIAHSKSPFIHQQFAQQLNIEHPYGRVLAPINDFI
                     NTLNAFFSAGGKGANVTVPFKEEAFARADELTERAALAGAVNTLKRLEDGRLLGDNTD
                     GIGLLSDLERLSFIRPGLRILLIGAGGASRGVLLPLLSLDCAVTITNRTVSRAEELTK
                     LFAHTGSIQALGMDELEGHEFDLIINATSSGISGDIPAIPSSLIHLGIYCYDMFYQKG
                     KTPFLAWCEQRGSKRNADGLGMLVAQAAHAFLLWHGVLPDVEPVIKLLQQELSA"
     gene            25467..25724
                     /locus_tag="J1Y83_002191"
     CDS             25467..25724
                     /locus_tag="J1Y83_002191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1488 domain-containing protein"
                     /protein_id="HBA7630338.1"
                     /translation="MNQAIQFPDREEWDENKRCVCFPALVNGMQLTCAISGESLAYRF
                     TGDTPEQWLASFRQHRWDLEEEAENLIQEQSEDDQGWVWLP"
     gene            complement(25700..26254)
                     /locus_tag="J1Y83_002192"
     CDS             complement(25700..26254)
                     /locus_tag="J1Y83_002192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HBA7630339.1"
                     /translation="MSDVLRPYRDLFPQIGQRVMIDDSSVVIGDVRLADDVGIWPLVV
                     IRGDVHYVQIGARTNIQDGSMLHVTHKSSYNPDGNPLTIGEDVTVGHKVMLHGCTIGN
                     RVLVGMGSILLDGAIVEDDVMIGAGSLVPQNKRLESGYLYLGSPVKQIRPLSDEEKAG
                     LRYSANNYVKWKDEYLDQGNQTQP"
BASE COUNT         6797 a         6250 c         6928 g         6604 t
ORIGIN      
        1 tattagtaat atgtatttaa ttaataaata catacattat ttttatgatt aaggataatc
       61 aataattaaa acaggaagtt ctatgtctac gagaagagaa gtcattcttt cctggttgcg
      121 tgagaaacgt cagacctggc gtctgtgcta tttgttgggt gaggctggaa gtggaaaaac
      181 ctggctggcg cagcaactac aaaaagataa acatcgccgt gtgattactt taagcctcgt
      241 tgtttcctgg caaggtaagg ccgcatggat cgttaccgac gataatgcgg ctgaacaggg
      301 ctgccgtgac agcgcctgga cgcgagatga gatggcgggt caattactgc atgcacttca
      361 tcgaactgat agtcgctgcc ctcttattat tattgaaaat gctcacctga atcaccgccg
      421 catacttgat gatctccaac gcgcgatatc tcttattcct gacggtcaat ttttgctgat
      481 cggcagaccc gatcgaaaag ttgaacgtga ttttaaaaaa cagggcattg aacttgtctc
      541 gacaggtcgt ctgatggagc acgagcttaa ggcaagcatc cttgaaggtc agaatattga
      601 ccagcccgat ctccttttaa ccgccagagt tctgaaacgg atagctttat tatgtcgggg
      661 agatcgcaga aagctggcgc ttgccggcga aacaattagc ttattacagc aagccgagca
      721 gacccgcgtt ttcactgcaa aacaatggcg aatgatttac cgcgtattag gtgataaccg
      781 cccccggaaa atgcagcttg ccgtggtgat gagtggaacc attatcgcac ttacctgtgg
      841 ttggctactg ctgtcctctt tcacagctcc gttacccgtt cctgcgtggc tgatacccgt
      901 aacgccagta gctaaacaag atatgactaa ggatattgcc catgtggtga tgcgcgatag
      961 tgaggcgcta agcgtgttat acggcgtatg ggggtacgag gtcccagccg attcggcctg
     1021 gtgcgatcag gcagtaagag cggggctggt atgtaaaagt gggaatgctt ctttacaaac
     1081 attggtggat cagaatttgc cctggatcgc atcgcttaaa gtcggggaca aaaaattgcc
     1141 tgttgtggtc gttcgcgtcg gtgatgctac cgttgatgta ctcgtagggc agcaaacctg
     1201 gacgctgaca cataaatggt ttgagttggt atggacgggt gattatcttc tgttgtggaa
     1261 aatgtcgccg gaaggggaga gtaccattac gcgtgatagc agcgaggaag agattctctg
     1321 gctggaaacg atgttaaacc gcgcattgca tatttcgaca gaatcttcgg cagagtggcg
     1381 tcccttgttg gtagaaaaaa taaagcaatt tcaaaaaagc catcacttga aaactgacgg
     1441 tgttgttggc ttttcaacgt tagtacatct ctggcaggtg gcgggggaga gtgcctactt
     1501 atatcgggat gaagcaaata tttccccaga aacaacagtg aaggggaaat gacatgtttg
     1561 aattctatat cgccgcgcgt gagcagaaag aaacgggtca ccctggtatc ttctcccgac
     1621 aaaaacacag caccataata tatgtcattt gtttattgct gatttgcctg tggtttgcag
     1681 gaatggtgtt ggttggtggg tatgcccggc agctatgggc actttggata gtaaaagcag
     1741 aagtcactgt agatgctgaa acgcccgctt ttaaacaatc aacacaacac tattttttca
     1801 aaaaacagcc actccccgtc gtcgaatccg tcgaggagga agatgaacca ggcgtcgcgg
     1861 ttgagaatgc cccctcttct tctgaagacg aagaaaatac tgtagaagaa agcgacgaga
     1921 aagccggttt aagagagcgt gtcaaaaatg cgctgaacga acttgagaga taacccaaag
     1981 gctgtttact ttactagcaa tacgcttgcg ttcggtggtt aagtatgtat aatgcgcggg
     2041 cttgtcgtag ttgacagcag gttcaatctg aacctcagtt agccgaattt ggctacctaa
     2101 caatgctccc aatcggggag ctacgtaaga acggttacac tctcccatca atcgtaatgg
     2161 gtctgaggag taatcatttt cgtttataaa ataattggag ctctggtctc atgcagaacc
     2221 aaagaatccg tatccgcctg aaagcgtttg atcatcgtct gatcgatcaa gcaaccgcgg
     2281 aaatcgtcga gactgctaag cgcactggtg cgcaggtccg tggtccgatc ccgctgccga
     2341 cacgcaaaga gcgcttcact gttctgatct ccccgcacgt caacaaagac gcgcgcgatc
     2401 agtacgaaat ccgtactcac ttgcgtctgg ttgacatcgt tgagccaacc gagaaaaccg
     2461 ttgatgctct gatgcgtctg gatctggctg ccggtgtaga cgtgcagatc agcctgggtt
     2521 aatcaggtca ttgagcgatt gagaggttga aacaatgatt ggtttagtcg gtaaaaaagt
     2581 gggtatgacc cgtatcttca cagaagacgg cgtttctatc ccagtaaccg taatcgaagt
     2641 tgaagcaaac cgcgttactc aggttaaaga cctggctaac gatggctacc gtgctattca
     2701 ggtgaccacc ggtgctaaaa aagctaaccg tgtgaccaag cctgaagctg gccacttcgc
     2761 taaagctggc gtagaagctg gccgtggtct gtgggaattc cgcctggctg aaggcgaaga
     2821 gttcactgta ggtcagagca ttagcgttga actgtttgct gacgttaaaa aagttgacgt
     2881 aactggcacc tctaaaggta aaggtttcgc aggtaccgtt aagcgctgga acttccgtac
     2941 ccaggacgct actcacggta actccttgtc tcaccgcgtt ccgggttcta tcggtcagaa
     3001 ccagactccg ggcaaagtgt tcaaaggcaa gaaaatggca ggtcagatgg gtaacgaacg
     3061 tgtaaccgtt cagagccttg acgtagtacg cgttgacgct gagcgcaacc tgctgctggt
     3121 taaaggcgct gtcccgggtg caaccggtag cgacctgatc gttaaaccag ctgtgaaggc
     3181 gtaaggagat agcaatggaa ttagtattga aagacgcgca gagcgcgctg actgtttccg
     3241 aaactacctt cggtcgtgat ttcaacgaag cgctggttca ccaggttgtt gttgcttatg
     3301 cagctggtgc tcgtcagggt actcgtgctc agaagactcg tgctgaagta actggttccg
     3361 gtaaaaaacc gtggcgccag aaaggcaccg gccgtgcgcg ttctggttct atcaagagcc
     3421 cgatctggcg ttctggtggc gtgacctttg ctgctcgtcc gcaggaccac agtcaaaaag
     3481 ttaacaagaa gatgtaccgc ggcgcgctga aaagcatcct gtccgaactg gtacgtcagg
     3541 atcgtctgat cgttgtcgag aagttctctg tagaagcgcc gaaaactaag ctgctggcac
     3601 agaaactgaa agacatggct ctggaagatg tgctgatcat caccggtgag ctggacgaga
     3661 acctgttcct ggctgcgcgc aacctgcaca aggttgacgt acgcgatgca actggtatcg
     3721 acccggttag cctgatcgcc ttcgacaaag tcgtaatgac tgctgatgct gttaagcaag
     3781 ttgaggagat gctggcatga ttcgtgaaga acgtctgctg aaggtgctgc gtgcaccgca
     3841 cgtttctgaa aaagcgtcta ctgcgatgga aaaatccaac accatcgtac tcaaagttgc
     3901 taaagacgcg accaaagcag aaatcaaagc tgctgtgcag aaactgtttg aagtcgaagt
     3961 cgaagtcgtt aacaccctgg tagttaaagg gaaagttaaa cgtcacggac agcgtatcgg
     4021 tcgtcgtagc gactggaaaa aagcttacgt caccctgaaa gaaggccaga atctggactt
     4081 cgttggcggc gctgagtaag tcggaggagt aatacaatgg cagttgttaa atgtaaaccg
     4141 acatctccgg gtcgtcgcca cgtagttaaa gtggttaacc ctgagctgca caagggcaaa
     4201 ccttttgctc cgttgctgga aaaaaacagc aaatccggtg gtcgtaacaa caatggccgt
     4261 atcaccactc gtcatatcgg tggtggccac aagcaggctt accgtattgt tgacttcaaa
     4321 cgcaacaaag acggtatccc agcagttgtt gaacgtcttg agtacgatcc gaaccgttcc
     4381 gcgaacatcg cgctggttct gtacaaagac ggtgaacgcc gttacatcct ggcccctaaa
     4441 ggcctgaaag ctggcgacca gattcagtct ggcgttgatg ctgcaatcaa accaggtaac
     4501 accctgccga tgcgcaacat cccggttggt tctactgttc ataacgtaga aatgaaacca
     4561 ggtaaaggcg gtcagctggc acgttccgct ggtacttacg ttcagatcgt tgctcgtgat
     4621 ggtgcttatg tcaccctgcg tctgcgttct ggtgaaatgc gtaaagtaga agcagactgc
     4681 cgtgcaactc tgggcgaagt tggcaatgct gagcatatgc tgcgcgttct gggtaaagca
     4741 ggtgctgcac gttggcgtgg tgttcgtccg accgttcgcg gtaccgcgat gaacccggta
     4801 gaccacccac atggtggtgg tgaaggtcgt aactttggta agcacccggt aactccgtgg
     4861 ggcgttcaga ccaaaggtaa gaagacccgc agcaacaagc gtactgataa attcatcgta
     4921 cgtcgccgta gcaaataatt ttagaggata agccatgcca cgttctctca agaaaggtcc
     4981 ttttattgac ctgcacttgc tgaagaaggt agagaaagcg gtggaaagcg gagacaagaa
     5041 gcccctgcgc acttggtccc gtcgttcaac gatctttcct aacatgatcg gtttgaccat
     5101 cgctgtccat aatggtcgtc agcatgttcc ggtatttgta accgacgaaa tggtcggtca
     5161 caaactgggt gaattcgcac cgactcgtac ttatcgcggc cacgctgctg ataaaaaagc
     5221 gaagaagaaa taaggtagga ggaagagatg gaaactatcg ctaaacatcg ccatgctcgt
     5281 tcttctgctc agaaggttcg ccttgttgct gacctgattc gcggtaagaa agtgtcgcag
     5341 gctctggata ttttgaccta caccaacaag aaagcggctg tactggtcaa gaaagttctg
     5401 gaatctgcca ttgctaacgc tgaacacaac gatggcgctg acattgacga tctgaaagtt
     5461 acgaaaattt tcgtagacga aggcccgagc atgaagcgca ttatgccgcg tgcaaaaggt
     5521 cgtgcagatc gcatcctgaa gcgcaccagc cacatcactg tggttgtgtc cgatcgctga
     5581 gactctggag actagcaatg ggtcagaaag tacatcctaa tggtattcgc ctgggtattg
     5641 taaaaccatg gaactctacc tggtttgcga acaccaaaga attcgctgac aacctggaca
     5701 gcgattttaa agtacgtcag tacctgacta aggaactggc taaagcgtcc gtatctcgta
     5761 tcgttatcga gcgtccggct aagagcatcc gtgtaaccat tcacactgct cgcccgggta
     5821 tcgttatcgg taaaaaaggt gaagacgtag aaaaactgcg taaggtcgta gcggacatcg
     5881 ctggcgttcc tgcacagatc aacatcgccg aagttcgtaa gcctgaactg gacgcaaaac
     5941 tggttgctga cagcatcact tctcagctgg aacgtcgcgt tatgttccgt cgtgctatga
     6001 agcgtgctgt acagaacgca atgcgtctgg gcgctaaagg tattaaagtt gaagttagcg
     6061 gccgtctggg cggcgcggaa atcgcacgta ccgaatggta ccgcgaaggt cgcgtaccgc
     6121 tgcacactct gcgtgctgac atcgactaca acacctctga agcgcacacc acttacggtg
     6181 taatcggcgt taaagtgtgg atcttcaaag gcgagatcct gggtggtatg gctgctgttg
     6241 aacaaccgga aaaaccggct gctcagccta aaaagcagca gcgtaaaggc cgtaaataag
     6301 gagcgtcgct gatgttacaa ccaaagcgta caaaattccg taaaatgcac aaaggccgta
     6361 accgcggtct ggcgcagggt acggatgtta gcttcggcag cttcggtctg aaagctgttg
     6421 gccgtggtcg tctgactgca cgtcagatcg aagcagcacg tcgtgctatg acccgtgcag
     6481 ttaagcgtca aggtaagatc tggatccgtg tgttcccgga caaaccgatc actgaaaagc
     6541 cgctggcagt gcgtatgggt aaaggtaaag gtaacgtgga gtattgggtt gccttgattc
     6601 agccgggtaa agtcctgtat gaaatggacg gtgttccgga agagctggcc cgtgaagcat
     6661 tcaagctggc agcagcgaaa ctgccgatta aaaccacctt tgtaactaag acggtgatgt
     6721 aatgaaagca aaagagctgc gtgagaagag cgttgaagag ctgaacaccg agctgctgaa
     6781 cctgctgcgt gagcagttca acctgcgtat gcaggctgca agtggccagc tgcaacagtc
     6841 tcacctgttg aagcaagtgc gtcgcgatgt cgcacgcgtt aagactttac tgaacgagaa
     6901 ggcgggtgcg taatgaccga taaaatccgt actctgcaag gtcgcgttgt tagcgacaaa
     6961 atggagaaat ccattgttgt tgctatcgaa cgttttgtga aacacccgat ctacggtaaa
     7021 ttcatcaagc gtacgaccaa actgcacgta catgacgaga acaacgaatg cggtatcggt
     7081 gacgtggttg aaatccgcga atgccgtccg ctgtccaaga ctaaatcctg gacgctggtt
     7141 cgcgttgtag agaaagcggt tctgtaatac agtacactct ctcgatacga ataaacggct
     7201 cagaaatgag ccgtttattt tttctaccca tatccttgaa gcggtgttat aatgccgcgc
     7261 cctcgatatg gggattttta acgacctgat tttcgggtct cagtagtagt tgacattagc
     7321 ggagcactaa aatgatccaa gaacagacta tgctgaacgt cgccgacaac tccggtgcac
     7381 gtcgcgtaat gtgtatcaag gttctgggtg gctcgcaccg tcgctacgca ggcgtaggcg
     7441 acatcatcaa gatcaccatc aaagaagcaa ttccgcgtgg taaggtcaaa aaaggtgatg
     7501 tgctgaaggc ggtagtggtg cgcaccaaga agggtgttcg tcgcccggac ggttctgtca
     7561 ttcgcttcga tggtaatgct tgtgttcttc tgaacaacaa cagcgagcag cctatcggta
     7621 cgcgtatttt tgggccggta actcgtgagc ttcgtagtga gaagttcatg aaaattatct
     7681 ctctggcacc agaagtactc taaggagcga atcatggcag cgaaaatccg tcgtgatgac
     7741 gaagttatcg tgttaaccgg taaagataaa ggtaaacgcg gtaaagttaa gaatgtcctg
     7801 tcttccggca aggtcattgt tgaaggtatc aacctggtta agaaacatca gaagccggtt
     7861 ccggccctga accaaccggg tggcatcgtt gaaaaagaag ccgctattca ggtttccaac
     7921 gtagcaatct tcaatgcgac aaccggcaag gctgaccgtg taggctttag attcgaagac
     7981 ggtaaaaaag tccgtttctt caagtctaac agcgaaacta tcaagtaatt tggagtagta
     8041 cgatggcgaa actgcatgat tactacaaag acgaagtagt taaaaaactc atgactgagt
     8101 ttaactacaa ttctgtcatg caagtccctc gggtcgagaa gatcaccctg aacatgggtg
     8161 ttggtgaagc gatcgctgac aaaaaactgc tggataacgc agcagcagac ctggcagcaa
     8221 tctccggtca aaaaccgctg atcaccaaag cacgcaaatc tgttgcaggc ttcaaaatcc
     8281 gtcagggcta tccgatcggc tgtaaagtaa ctctgcgtgg cgaacgcatg tgggagttct
     8341 ttgagcgcct gatcactatt gctgtacctc gtatccgtga cttccgtggc ctgtccgcta
     8401 agtctttcga cggtcgtggt aactacagca tgggtgtccg tgagcagatc atcttcccag
     8461 aaatcgacta cgataaagtc gaccgcgttc gtggtttgga tattaccatt accactactg
     8521 cgaaatctga cgaagaaggc cgcgctctgc tggctgcctt tgacttcccg ttccgcaagt
     8581 aaggtagggt tactaaatgg ctaagcaatc aatgaaagca cgcgaagtaa aacgcgtagc
     8641 tttagctgat aaatacttcg cgaaacgcgc tgaactgaaa gcgatcatct ctgatgtgaa
     8701 cgcttccgac gaagatcgtt ggaacgctgt tctcaagctg cagactctgc cgcgtgattc
     8761 cagcccgtct cgtcagcgta accgctgccg tcaaacaggt cgtccgcatg gtttcctgcg
     8821 gaagttcggg ttgagccgta ttaaggtccg tgaagccgct atgcgcggtg aaatcccggg
     8881 tctgaaaaag gctagctggt aattgtcacc aattgaatca cgggagtaaa gacagatgag
     8941 catgcaagat ccgatcgcgg atatgctgac ccgtatccgt aacggtcagg ccgcgaacaa
     9001 agctgcggtc accatgcctt cctccaagct gaaagtggca atcgccaacg tgctgaagga
     9061 agaaggtttt attgaagatt ttaaagttga aggcgacacc aagcctgaac tggaacttac
     9121 tctgaagtat ttccagggca aagctgttgt agaaagcatt cagcgtgtca gccgcccagg
     9181 tctgcgcatc tataaacgta aagatgagct gccgaaagtt atggcgggtc tgggtatcgc
     9241 agttgtttct acctctaaag gtgttatgac tgatcgtgca gcgcgccagg ctggtcttgg
     9301 tggcgaaatt atctgctacg tagcctaatc ggaggaaaaa atgtctcgtg ttgctaaagc
     9361 accggtcgtt gttcctgccg gcgttgacgt aaaaatcaac ggtcaggtta ttacgatcaa
     9421 aggtaaaaac ggcgagctga ctcgtactct caacgatgct gttgaagtta aacatgcaga
     9481 taataccctg accttcggtc cgcgtgatgg ttacgcagac ggttgggcac aggctggtac
     9541 cgcgcgtgcc ctgctgaact caatggttat cggtgttacc gaaggcttca ctaagaagct
     9601 gcagctggtt ggtgtaggtt accgtgcagc ggttaaaggc aatgtgatta acctgtctct
     9661 gggtttctct catcctgttg accatcagct gcctgcgggt atcactgctg aatgtccgac
     9721 tcagactgaa atcgtgctga aaggcgctga taagcaggtg atcggccagg ttgcagcgga
     9781 tctgcgcgcc taccgtcgtc ctgagcctta caaaggcaag ggtgttcgtt acgccgacga
     9841 agtcgtgcgt accaaagagg ctaagaagaa gtaaggtaac actatggata agaaatctgc
     9901 tcgtatccgt cgtgcgaccc gcgcacgccg caagctccag gagctgggcg caactcgcct
     9961 ggtggtacat cgtaccccgc gtcacattta cgcacaggta attgcaccga acggttctga
    10021 agttctggta gctgcttcta ctgtagaaaa agctatcgct gaacaactga agtacaccgg
    10081 taacaaagac gcggctgcag ctgtgggtaa agctgtcgct gaacgcgctc tggaaaaagg
    10141 catcaaagat gtatcctttg accgttccgg gttccaatat catggtcgtg tccaggcact
    10201 ggcagatgct gcccgtgaag ctggccttca gttctaaggt agaggtgtaa gatggctcac
    10261 atcgaaaaac aagctggcga actgcaggaa aagctgatcg cggtaaaccg cgtatctaaa
    10321 accgttaaag gtggtcgtat tttctccttc acagctctga ctgtagttgg cgatggtaac
    10381 ggtcgcgttg gttttggtta cggtaaagcg cgtgaagttc cagcagcgat ccagaaagcg
    10441 atggaaaaag cccgtcgcaa tatgattaac gtcgcgctga ataacggcac tctgcaacac
    10501 cctgttaaag gtgttcacac gggttctcgc gtattcatgc agccggcttc cgaaggtacc
    10561 ggtatcatcg ccggtggtgc aatgcgcgcc gttctggaag tcgctggggt tcataacgtt
    10621 ctggctaaag cgtatggttc caccaacccg attaacgtgg ttcgtgcaac tatcgatggc
    10681 ctggaaaata tgaattctcc agaaatggtc gctgccaagc gtggtaaatc cgttgaagaa
    10741 attctgggga aataaaccat ggcaaagact attaaaatta ctcaaacccg cagtgcaatc
    10801 ggtcgtctgc cgaaacacaa ggcaacgctg cttggcctgg gtctgcgtcg tattggtcac
    10861 accgtagagc gcgaggatac tcctgctatt cgcggtatga tcaacgcggt ttccttcatg
    10921 gttaaagttg aggagtaaga gatgcgttta aatactctgt ctccggccga aggctccaaa
    10981 aaggcgggta aacgcctggg tcgtggtatc ggttctggcc tcggtaaaac tggtggtcgt
    11041 ggtcacaaag gtcagaaatc tcgttctggc ggtggcgtac gtcgcggttt cgagggtggt
    11101 cagatgcctc tgtaccgtcg tctgccgaaa ttcggcttca cttctcgtaa agcagcgatt
    11161 acagccgaag ttcgtctgtc tgacctggct aaagtagaag gcggtgttgt agacctgaac
    11221 acgctgaaag cggctaacat tatcggtatc cagatcgagt tcgcgaaagt gatcctggct
    11281 ggcgaagtaa cgactccggt aactgttcgt ggcctgcgtg ttactaaagg cgctcgtgct
    11341 gctatcgaag ctgctggcgg taaaatcgag gaataagtag cagatggcta aacaaccggg
    11401 attagatttt caaagtgcca aaggtggctt aggcgagctg aaacgcagac tgctgtttgt
    11461 tatcggtgcg ctgattgtgt tccgtattgg ctcttttatt ccgatccctg gtattgatgc
    11521 cgctgtactt gccaaactgc ttgagcaaca gcgaggcacc atcattgaga tgtttaacat
    11581 gttctctggt ggtgctctca gccgtgcttc tatctttgct ctggggatca tgccgtatat
    11641 ttcggcgtcg atcattatcc agctactgac ggtggttcat ccaacgttgg cagaaattaa
    11701 gaaagaaggg gagtctggtc gtcgtaagat cagccagtac acccgctacg gtactctggt
    11761 gctggcaata ttccagtcga tcggtattgc tactggtctg ccgaatatgc ctggtatgca
    11821 aggcctggtg attaacccgg gctttgcatt ctacttcacc gctgttgtaa gtctggtcac
    11881 aggaaccatg ttcctgatgt ggttgggcga acagatcact gaacgaggta tcggcaacgg
    11941 tatttcaatc attatcttcg ccggtattgt cgcgggactc ccgccagcca ttgcccatac
    12001 tatcgagcaa gcgcgtcaag gcgacctgca cttcctcgtg ttgctgttgg ttgcagtatt
    12061 agtatttgca gtgacgttct ttgttgtatt tgttgagcgt ggtcaacgcc gcattgtggt
    12121 aaactacgcg aaacgtcagc aaggtcgtcg tgtctatgct gcacagagca cacatttacc
    12181 gctgaaagtg aatatggcgg gggtaatccc ggcaatcttc gcttccagta ttattctgtt
    12241 cccggcgacc atcgcgtcat ggttcggggg cggtactggt tggaactggc tgacaacaat
    12301 ttcgctgtat ttgcagcctg ggcaaccgct ttatgtgtta ctctatgcgt ctgcaatcat
    12361 cttcttctgt ttcttctaca cggcgttggt tttcaacccg cgtgaaacag cagataacct
    12421 gaagaagtcc ggtgcatttg taccaggaat tcgtccggga gagcaaacgg cgaagtatat
    12481 cgataaagta atgacccgcc tgaccttggt tggtgcgctg tacattacct ttatctgcct
    12541 gatcccggag ttcatgcgtg atgcaatgaa agtaccgttc tacttcggtg ggacctcact
    12601 gcttatcgtt gttgtcgtga ttatggactt tatggctcaa gtgcaaactc tgatgatgtc
    12661 cagtcagtat gagtctgcat tgaagaaggc gaacctgaaa ggctacggcc gataattggt
    12721 cgcccgagaa gttacggaga gtaaaaatga aagttcgtgc ttccgtcaag aaattatgcc
    12781 gtaactgcaa aatcgttaag cgtgatggtg tcatccgtgt gatttgcagt gccgagccga
    12841 agcataaaca gcgccaaggc tgattttttc gcatattttt cttgcaaagt tgggttgagc
    12901 tggctagatt agccagccaa tcttttgtat gtctgtgcgt ttccatttga gtatcctgaa
    12961 aacgggcttt tcagcatgga acgtacatat taaatagtag gagtgcatag tggcccgtat
    13021 agcaggcatt aacattcctg atcataagca tgccgtaatc gcattaactt cgatttatgg
    13081 cgtcggcaag acccgttcta aagccatcct ggctgcagcg ggtatcgctg aagatgttaa
    13141 gatcagtgag ctgtctgaag gacaaatcga cacgctgcgt gacgaagttg ccaaatttgt
    13201 cgttgaaggt gatctgcgcc gtgaaatcag catgagcatc aagcgcctga tggatcttgg
    13261 ttgctatcgc ggtttgcgtc atcgtcgtgg tctcccggtt cgcggtcagc gtaccaagac
    13321 caacgcacgt acccgtaagg gtccgcgcaa accgatcaag aaataatcgg ggtgattgaa
    13381 taatggcaaa ggcaccaatt cgtgcacgta aacgtgtaag aaaacaagtc tctgacggcg
    13441 tggctcatat ccatgcttct ttcaacaaca ccatcgtgac tatcactgat cgtcagggta
    13501 acgcgttggg ttgggcaaca gccggtggtt ccggtttccg tggttctcgc aaatccactc
    13561 cgtttgcagc acaggttgca gcagagcgtt gcgctgacgc cgtgaaagaa tacggcatca
    13621 agaatctgga agttatggtt aaaggtccgg gtccaggccg cgaatctact attcgtgctc
    13681 tgaacgccgc aggtttccgc atcactaaca ttactgatgt gactccgatc cctcataacg
    13741 gttgtcgtcc gccgaaaaaa cgtcgcgtat aacgcctcgt tttccaggtt tgttggagaa
    13801 agaaaatggc aagatatttg ggtcctaagc tcaagctgag ccgtcgtgag ggcaccgact
    13861 tattccttaa gtctggcgtt cgcgcgatcg ataccaagtg taaaattgaa caagctcctg
    13921 gccagcacgg tgcgcgtaaa ccgcgtctgt ctgactatgg tgtgcagttg cgtgaaaagc
    13981 aaaaagttcg ccgtatctat ggtgtgctgg agcgtcagtt ccgtaactac tacaaagaag
    14041 cagcacgtct gaaaggcaac accggtgaaa acctgttggc tctgctggaa ggtcgtctgg
    14101 acaacgttgt ataccgtatg ggcttcggtg ccactcgtgc agaagcacgt cagctggtta
    14161 gccataaagc aattatggta aacggtcgtg ttgttaacat cgcttcttat caggttagtc
    14221 cgaatgacgt tgtaagcatt cgtgagaaag cgaagaagca gtctcgcgtg aaagccgctc
    14281 tggagctggc tgagcagcgt gaaaagccaa cctggctgga agttgatgct ggcaagatgg
    14341 aaggtacgtt taagcgtaag ccggagcgtt ctgatctgtc tgcggacatt aacgaacacc
    14401 tgatcgtcga gctttactcc aagtaaagct tagtaccaaa gagaggacac aatgcagggt
    14461 tctgtgacag agtttctaaa accgcgcctg gttgatatcg agcaagtgag ttcgacgcac
    14521 gccaaggtga cccttgagcc tttagagcgt ggctttggcc atactctggg taacgcactg
    14581 cgccgtattc tgctctcatc gatgccgggt tgcgcggtga ccgaggttga gattgatggt
    14641 gtactacatg agtacagcac caaagaaggc gttcaggaag atatcctgga aatcctgctc
    14701 aacctgaaag ggctggcggt gagagttcag ggcaaagatg aagttattct taccttgaat
    14761 aaatctggca ttggccctgt gactgcagcc gatatcaccc acgacggtga tgtcgaaatc
    14821 gtcaagccgc agcacgtgat ctgccacctg accgatgaga acgcgtctat tagcatgcgt
    14881 atcaaagttc agcgcggtcg tggttatgtg ccggcttcta cccgaattca ttcggaagaa
    14941 gatgagcgcc caatcggccg tctgctggtc gacgcatgct acagccctgt ggagcgtatt
    15001 gcctacaatg ttgaagcagc gcgtgtagaa cagcgtaccg acctggacaa gctggtcatc
    15061 gaaatggaaa ccaacggcac aatcgatcct gaagaggcga ttcgtcgtgc ggcaaccatt
    15121 ctggctgaac aactggaagc tttcgttgac ttacgtgatg tacgtcagcc tgaagtgaaa
    15181 gaagagaaac cagagttcga tccgatcctg ctgcgccctg ttgacgatct ggaattgact
    15241 gtccgctctg ctaactgcct taaagcagaa gctatccact atatcggtga tctggtacag
    15301 cgtaccgagg ttgagctcct taaaacgcct aaccttggta aaaaatctct tactgagatt
    15361 aaagacgtgc tggcttcccg tggactgtct ctgggcatgc gcctggaaaa ctggccaccg
    15421 gcaagcatcg ctgacgagta accggatcac aggttaaggt tttactgaga aggataaggt
    15481 catgcgccat cgtaagagtg gtcgtcaact gaaccgcaac agcagccatc gccaggctat
    15541 gttccgcaat atggcaggtt cactggttcg tcatgaaatc atcaagacga ctctgcctaa
    15601 agcgaaagag ctgcgccgcg tagttgagcc gctgattact cttgccaaga ctgatagcgt
    15661 tgctaatcgt cgtctggcat tcgcccgtac tcgtgataac gagatcgtgg caaaactgtt
    15721 taacgaactg ggcccgcgtt tcgcgagccg tgccggtggt tacactcgta ttctgaagtg
    15781 tggcttccgt gcaggcgaca atgcgccgat ggcttacatc gagctggttg atcgttcaga
    15841 gaaagcagaa gctgctgcag agtaatctga agcaacgtaa aaaaacccgc cccggcgggt
    15901 ttttttatac ccgtagcatc cccacttatc tacaatagct gtactctttt tgttcatccc
    15961 ctggagtatt tatgtggtta cttgaccagt gggcagagcg tcatatagca gaagcgcaag
    16021 cgaaaggtga gtttgataac ctggtaggta gcggcgaacc attgatactg gatgatgatt
    16081 ctcatgtgcc accggaatta cgtgcggggt atcgcttgct gaagaatgct ggttgcttac
    16141 cgccagaact tgagcatagg agggaagcaa tacagcttct ggatattctc aaaggtatcc
    16201 gtcatgatga tccgcaatat caagaggtta gtcgtcgatt gtcattactg gaattgaagc
    16261 tgcgacaagc tggattgagt accgattttt tacgcggcga ttatgctgac aagttgttga
    16321 acaaaatcaa cgataactag tggagtatgt atgtatcgca ttggtgagct ggcaaaaatg
    16381 gcggaagtaa cacccgacac gattcgttat tacgaaaagc agcagatgat ggagcatgaa
    16441 gtacgcaccg aaggtgggtt tcgcctgtat accgaaagcg atctccagcg attgaaattt
    16501 atccgccatg ccagacaact aggtttcagt ctggagtcga tccgcgagtt gctgtcgatc
    16561 cgtatcgatc ctgaacacca tacctgtcag gagtcaaaag gcattgtgca ggaaagattg
    16621 caggaagtcg aagctcgtat agccgagttg cagagtatgc agcgttcctt gcaacgcctt
    16681 aatgatacct gttgtgggac cgcccatagc agtgtttatt gctcgattct tgaagctctt
    16741 gaacaagggg cgagtggcgt taagagtggc tgttgatttt ttgcactggc aggattacac
    16801 tcgcgccgtt aaataaccaa ttggagtttt tatgagccga tatcagcata ctaaagggca
    16861 gataaaggat aatgcgatag aagcattact acatgatccc ttattccgac agcgagtaga
    16921 gaaaaataag aaagggaaag gcagttacat gcgaaaagga aaacatggca atcgggggaa
    16981 ctgggaggcc agtggcaaga aagtaaatca cttttttacc actggccttc tgctttcaga
    17041 cgcttgttaa gagcggttat tctgctcttt cagcaaatca cgaatttctg tcagtaatac
    17101 ttcttcttta gttggtgcag gtgttgctgc tgctggttct tcttttttcc gattcagttt
    17161 gttgattagc ttaatcgcca taaagatggc aaaggccact atcagaaaat caaagacgtt
    17221 ttgaatgaag acaccgtaat gcatcacaac agcagggata tccccctgcg catcgcgtag
    17281 cgtgacagca aactgtttga aatcgatccc gccaattaat aaccccagag gtggcataat
    17341 gatatcggca accagtgaag atacaatctt cccgaatgcc gcaccgataa tgacacccac
    17401 cgccaaatcc accacgttcc cgcgcatcgc aaattcgcga aattctttaa taatgctcat
    17461 gttattctcc ctatgaagcc gacaatcata agtctaacaa atgttaagct attttcctgc
    17521 caggagtgaa tttaattaat ccctgataat caaagggaaa taataaggcg tcattagacg
    17581 ccttattaat tacaagaaga aaggacttgg ctggaagagt ctttcgacgt cggtaataaa
    17641 ctttttatct gtgaggaaca taattacgtg atcgccttgc tcaatgcgca gattgtcatt
    17701 ggcaatcatc acatcgtttc cacgtaccac cgctccaata atcgttcctg gcggtagctt
    17761 gatttcgtca atgactctgc cgacaacgcg tgaggtgctt tcatcaccgt gagcaacggc
    17821 ttcaatagct tctgctacgc cgcggcgtaa tgaggaaaca ccaacaatat ctgcttttcg
    17881 cacatggcta agcaacgcag aaatagttgc ttgttgtggt gaaatcgcaa tatcgatcac
    17941 gctcccctga accagatcca cataagcgcg acgttggatc aataccatca cctttttcgc
    18001 ccccatacgt ttggcaagca tggcggacat gatattggcc tcgtcatcgt tggtgacggc
    18061 aataaacaga tcaacttgat cgatatgttc ttcggccagc agttcttgat ccgacgcatc
    18121 accaaaaaag acgatcgtat tctgcaactt ttccgccagt tcggcagcgc gctgctgatt
    18181 acgttcgatg agtttaacgc tgtaatcttt ttccagacga cgcgccagcc ctgcaccgat
    18241 attaccgcca ccaaccagca tgatccgctt atacggtttt tccagtcgct gtaattcact
    18301 catcaccgcg cggatatgct gtgaagcggc aataaagaac acttcatcac cagcttcaac
    18361 aatggtcgaa ccttgcggac gaatggggcg atcgtggcgg aaaattgctg ccacacgagt
    18421 atcgatatgt ggcatatgtt cgcgcatggt cgaaagtgca ttaccaatca gcgggccgcc
    18481 ataataggct ttaaccacag ccaggctgac tttaccctca gcgaagttca ccacctgcaa
    18541 tgcgccggga tactcaatca gtcggtaaat attatcgata accaactgct ctggtgcgat
    18601 cagatgatca atcggtacag catctgaatg aaatagttta tcggcatcgc gcacgtagtc
    18661 tggtgagcgg atacgagcga tgcgattagg ggtgttgaaa agtgagtagg ctacctggca
    18721 ggcaaccata ttggtttcat ctgaactggt tacagcaacc agcatatcgg cgtcgtcggc
    18781 acctgcctcc cgcaatacgc gtggatgaga gccatgcccc tgcacgaccc gcaggtcaaa
    18841 tttatcctgt aaggtccgca gacgctcacc gttggtatcg acaacagtaa tatcgttgtt
    18901 ctcgccaacc aggttttccg ccagtgtgcc gccaacctgg ccggcaccca gaatgataat
    18961 tttcatcagt cgcgacccgt tatctcatca ctttttgatt agcttagcgt aaaagaagcc
    19021 gtcgccctct tcggcaccag gcagattttg tttacccggt tgctctggtg ttcctgtttc
    19081 gcaaagttcg gcatcagcgg tacgttgcaa gaaggcttta atctgcaggc tattctcttc
    19141 cggtaacact gaacaggtgg catagaccag ggttccaccg gattttaaat gtggccaaat
    19201 ggcgtcgaga atttcagact gcaattgcgc gagttccggg atatcgcgat cacgacgtaa
    19261 ccatttaata tccggatggc gacgaatcac ccccgttgct gaacaaggcg catctaataa
    19321 aatgcgatca aactgttgct cgccacacca ttgggaaggg taacggccat caccttgttt
    19381 cacggttgcc ttcataccaa ggcgttttaa attgtcgtaa acgcgagaga ggcgctgttc
    19441 gtcaatatca accgcaacaa cctgcgcttc tggtgccacc tcaaggatat gcgttgtttt
    19501 accgccgggg gcggcacaaa gatccaaaat gtgttcaccg ttttgtggcg caagccaggt
    19561 catgcaacct tgtgctgagg catcctgaac ggtaacccat ccgtcttcaa aaccaggtag
    19621 cgcatgaaca ggtgcaggtg tttccagacg tacagcatca gggtaatccg catgcgggaa
    19681 acctttcatt cctgcttcat ccagcaatgc aagccagctg tcgcgggaat gatgcgtacg
    19741 attaacgcgc agccacattg gcggacgctg gttattggct tcgacgatgg attgccactg
    19801 ctcgggatac gccttttgca gacgcttcag caaccaggaa ggatgcagat aacgtgcatc
    19861 actggcatta aactcggcta ataactcatc ttgctgacgc tggaactgac gtaatacacc
    19921 gttaatcaat cccttaagtt gcgggcgctt aattgcaact gcgccttcaa ccgtttcagc
    19981 cagcgcggca tgaggtggaa tgcgggtata aagcagttga tacaaaccaa ccataatcag
    20041 gtaatgaaca gtacgctgtt tgcctgtcat cggacgggcc attagcttat taatcagcca
    20101 gtctaactgt gagagcgtac gcagtacgcc aaagcataac tcctggagaa gagctttatc
    20161 tttatcggaa actttttgct ggagtggtgg cagaatgttg ctcagtgatt gcccttgctc
    20221 aacgacttgt tcaatggcct gggccgccat gctacgtaaa ttacgttgtt ttttcataag
    20281 tataaatata aagatgcccg gtaagaccgg gcttagaaga gtggactatc aggccagacg
    20341 gttgcccggc acaaaccatt cccgacgaga attcaggagg tcttgcacgc tcatcgcttt
    20401 cttacctgca ggttgcattg agagcagatt caggatgcca tcacctgtcg ctacctgaat
    20461 gccttgttta ttggcttcaa ggattgttcc tggcgcagcc tttgttgtcg tatcgatgac
    20521 agatgctttc cagactttaa ccggctgccc ttcaatttcc agccagctca ttggccatgg
    20581 attgaaagcg cgaatgcaac gttcaagctg tgctgccgaa agtgaccagt caatacgcgc
    20641 ttcttcttta ctcaactttt cggcgtaagt gacaagagtt tcgtcctgaa cttctggttt
    20701 cgccgtgccg tctgccagtt gtttcaacgt agtaataagc ccttgtgggc caagctctgc
    20761 cagtttgtcg tacagcgtac cactggtatc ttctgcagta atcgggcagg agagcttata
    20821 gagcatgtca ccggtgtcta aaccaatatc catttgcata atggtcacac cagtttctgt
    20881 atcacccgcc catagtgagc gttggattgg tgcagcaccg cgccagcgtg gcagcagtga
    20941 accatgaacg ttgatacagc caagacgcgg catctccagc actgctttag gcagaattaa
    21001 gccataggcg acgacaacca taacatcagc ctgtagatcg gcgaccagtt gctggttttc
    21061 ttgtggacgc agggaaacag gttgaaaaac gggcagacct ttgtcctcag ccagaacttt
    21121 aaccgggctg ggcatcagtt ttttaccgcg tcctgccggt cggtctggct gggtgaacac
    21181 gccaacgatg ttatgaccag aagacaacag cgcgtcgaga tgacgcgctg caaagtcagg
    21241 tgtacccgca aaaataatac gtagtgattc tgacacgtta attcttatcc ttaagcccgg
    21301 gctttcagac gatccagttt ttcaactttc tgacgaatac gttgttgttt cagcggtgac
    21361 agataatcca taaacagttt gccgaccagg tgatccatct catgctgaat acagatggct
    21421 aacagaccgt ctgcttccag ttcaaatggt ttaccgtcgc ggtccaaggc gcgaatttta
    21481 actttctctg cgcgtggcac taaagcacgt tgttcaggga tcgacaggca accttcttca
    21541 atgcctgttt cgccgctttt ttctaaaagc tccggattga ttaacaccag ccgttcgtca
    21601 cggttttccg aaacatcaat tacaatgata cgttgatgga tatcaacctg ggttgccgcc
    21661 aggccaatac cttcttctgc gtacatcgtc tcgaacatat catcgacgat acgctgaatt
    21721 tctgcattca cttcttctac cggtttagca actttgcgaa gccgctcgtc cggaatatgt
    21781 aacacttgca aaactgacat aaatctccag agatgtgttc aggagttaga aagattattt
    21841 cttctattct agacaaatcc ccctctgatt gacagcatca ctgaccaatc gcaaagattg
    21901 ctaaggctgc ttatggcagg gagataagga tggtcgatac agaaatttgg ctgcgtttaa
    21961 ttagcatcag cagcttgtat ggcgatgata tggtccgtat agctcactgg ctggcaaaac
    22021 agtcgcatat tgatgcggtt gtattgcagc aaacagggct tacattgcgg caggcacaac
    22081 gctttctttc atttccgcga aagagtatcg aaagctcact ttgttggttg gagcaaccca
    22141 accatcattt aatccctgcg gacagcgaat tttatcctcc tcaacttctg gcgacgacag
    22201 attaccccgg cgcactgttt gttgaaggag aactgcacgc gttgcattca tttcagcttg
    22261 ccgtagtggg gagtcgggcg cattcatggt atggcgagcg atggggacga ttattttgcg
    22321 aaactctggc gacgcgtgga gtgacaatta cgagtggact ggcgcgtgga atcgatggtg
    22381 tggcgcataa agcggcctta caggtaaatg gcgttagcat tgctgtattg gggaatggac
    22441 ttaataccat tcatccccgc cgccatgctc cactggctgc cagtctactt gagcaaggtg
    22501 gtgctctcgt ctcggaattt cccctcgatg ttccacccct tgcttacaat ttcccacgaa
    22561 gaaatcgcat tatcagtggc ctaagtaaag gtgtactggt ggtggaagcg gctttgcgca
    22621 gtggttcgct ggtgacagca cgttgtgcgc ttgagcaggg gcgtgaagtt tttgctttgc
    22681 caggtccaat agggaatccg ggaagcgaag ggcctcactg gttaataaaa cagggggcga
    22741 ttcttgtgac ggaaccggaa gaaattctgg aaaacttgca atttggattg cactggttgc
    22801 cagacgcccc tgaaaattca ttttattcac cagatcagga agacgtggca ttgccatttc
    22861 ctgagctcct ggctaacgta ggagatgagg taacacctgt tgacgtcgtc gctgaacgtg
    22921 ccggccaacc tgtgccagag gtagttactc aactactcga actggagtta gcaggatgga
    22981 tcgcagctgt acccggcggc tatgtccgat tgaggagggc atgccatgtt cgacgtacta
    23041 atgtatttgt ttgaaaccta tattcacaca gaagctgagt tgcgtgtgga tcaagacaaa
    23101 cttgaacagg atcttaccga cgcaggattt gatcgagaag atatctacaa tgccctgctt
    23161 tggctggaaa agcttgcgga ttatcaggaa ggactggcag aaccgatgca actggcctct
    23221 gatccactct ccatgcgtat ttatacccct gaagagtgtg aaagactgga tgccagctgt
    23281 cgtggttttc tgcttttcct tgagcaaatt caggtgctca accttgaaac ccgtgaaatg
    23341 gtgatagagc gagtgctcgc gctggatacc gctgagttcg acctggaaga cctgaaatgg
    23401 gtaatcctga tggtgttgtt caatattccg ggctgcgaaa atgcgtacca gcaagtggaa
    23461 gaattactct ttgaagtgaa tgaaggtatg ctgcattaat catttcttta attcagcata
    23521 agtagttatg gcgaaatcag cactgttcac ggtgcgtaat aatgagtcct gcccaaagtg
    23581 cggggctgaa ctagttattc gatccgggaa acacggtccg tttcttggat gctcacagta
    23641 tccggcgtgt gactacgtcc gtcctctgaa atcttcagcg gatggacata tcgtcaaagt
    23701 tctggagggg caggtttgcc ctgcatgtgg cgcaaatctg gtattacgcc agggacgctt
    23761 tggtatgttt attggttgca gtaactaccc tgaatgcgaa cataccgaac ttatcgataa
    23821 accggacgaa acagcaatta catgccccca atgccggacg ggccatctgg ttcagcgccg
    23881 ctcccgttat ggcaaaacat ttcactcctg tgatcgctac ccggagtgtc aatttgccat
    23941 taacttcaaa cccatagctg gagaatgccc tgagtgtcat tatccgctac tcatcgaaaa
    24001 gaaaaccgcg cagggtgtaa aacacttttg tgccagtaaa caatgtggaa agccgatttc
    24061 ggcggaataa taacgtgaat aataacctgc aaggagacgc tatcgcagct gcgatagatg
    24121 tcctcaatga agaacgtgtc atcgcctatc caacggaagc cgttttcggt gtcgggtgcg
    24181 atcctgatag cgaaacagca gtgatgagac tgttggagtt aaaacggcgt ccggttgata
    24241 aggggctgat tttaatcgcg gcaaattacg agcagcttaa accctatatt gatgacacca
    24301 tgctgactga cgcgcagcgt gaaaccattt tttcccgctg gccaggtcct gtcacctttg
    24361 tctttcccgc gcctgcgaca acaccgcgct ggttgacggg ccgctttgat tcgcttgctg
    24421 tacgagtcac cgaccatccg ttggtggttg ctttgtgcca ggcttatggt aaaccgctgg
    24481 tttctaccag tgccaacttg agtggattgc caccttgtcg aacagtagac gaagttcgcg
    24541 cacaatttgg cgcggcgttc ccggttgtgc ctggtgaaac gggggggcgt ttaaatcctt
    24601 cagaaatccg cgatgccctg acgggtgaac tgtttcgaca ggggtaacat aatggaaacc
    24661 tatgctgttt ttggtaatcc gatagcccac agcaaatcgc cattcattca tcagcaattt
    24721 gctcagcaac tgaatattga acatccctat gggcgcgtgt tggcacccat caatgatttc
    24781 atcaacacac tgaacgcttt ctttagtgct ggtggtaaag gtgcgaatgt gacggtacct
    24841 tttaaagaag aggcttttgc cagagcggat gagcttactg aacgggcagc gttggctggt
    24901 gctgttaata ccctcaagcg gttagaagat ggacgcctgc tgggtgacaa taccgatggt
    24961 ataggtttgt taagcgatct ggaacgtctg tcttttatcc gtcctggttt acgtattctg
    25021 ctcattggcg ctggtggagc atctcgcggc gtactactgc cactcctttc cctggactgt
    25081 gcggtgacaa tcactaatcg gacggtatct cgcgcagaag agttgactaa attgtttgcg
    25141 cacactggca gtattcaggc gttgggtatg gacgaactgg aaggtcatga gtttgatctc
    25201 attattaatg caacatccag tggcatcagt ggtgatattc cggcgatccc gtcatcgctc
    25261 attcatctag gcatttattg ctatgacatg ttctatcaga aaggaaaaac tccttttctg
    25321 gcatggtgtg agcagcgagg ctcaaagcgt aatgctgatg gtttaggaat gctggtggca
    25381 caggcggctc atgccttcct tctttggcat ggcgttctgc ctgatgtaga accggtcatc
    25441 aaactattgc aacaggaatt gtcagcgtga atcaggccat ccagtttccg gatagggaag
    25501 agtgggacga gaataaaaga tgtgtatgtt ttcccgctct cgtgaatggt atgcaactga
    25561 catgtgcgat ctctggcgag agtctggcgt atcgctttac tggagatacg ccagaacagt
    25621 ggttagcgag ttttcgtcag catcgctggg acctggaaga agaagcggaa aacttaattc
    25681 aggaacaaag tgaagatgat caaggttggg tctggttacc ttgatccaga tattcgtcct
    25741 tccatttcac gtagttattt gcggaatagc gtaacccagc cttctcttca tcacttaacg
    25801 ggcggatctg tttgacgggg ctaccgagat acagatatcc gctctccagc cgtttatttt
    25861 gtgggaccag gctacccgca ccaatcatca catcatcttc tactattgcg ccatcaagta
    25921 aaattgagcc catcccaacc aaaactcgat tgccaatggt gcagccgtgg agcatcacct
    25981 tgtgaccaac agtgacatct tcgccaatgg ttaatgggtt gccatctggg ttgtacgagg
    26041 atttatgagt gacatgcaac atactgccat cctggatatt ggtgcgtgct ccgatctgta
    26101 cataatgtac atctccacga atcacaacga gcggccagat ccccacatca tcagccagac
    26161 gaacgtcacc aatcacgacg ctgctatcgt cgatcattac gcgctgaccg atttgtggaa
    26221 aaagatcgcg gtatgggcgt aaaacatcag acatactcac ctcaacaata aatgatttcc
    26281 taatgacttt gggtgcatta ttggccttgt gcaagtcttt tagtatgcaa aaaagcaccg
    26341 ttttgtgtgc gaatgcagca aaaagggtga aaaaacaaca aacagaaaaa aagatcaaaa
    26401 aagtacttgt gcaaaaaatt gggatcccta taatgcgcct ccgttgagac gacaatgtga
    26461 aacacttcac aagatggtcg taacaacaaa gagaaaaaat cctgaaattc agggttgact
    26521 ctgaaagagg aaagcgtaat atacgccacc tcgcgacagt gagctgaaag ccgcgtcgc
//
LOCUS       DADSYM010000063        25806 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2415
            SAMN08039579-rid11723173.denovo.063, whole genome shotgun sequence.
ACCESSION   DADSYM010000063 DADSYM010000000
VERSION     DADSYM010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039579
            Sequence Read Archive: SRR6362533
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 25806)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25806)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980162.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 03:39:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,484
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 4,347
            CDSs (with protein)               :: 4,347
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 43 of 85
            Pseudo Genes (incomplete)         :: 37 of 85
            Pseudo Genes (internal stop)      :: 19 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25806
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039579-rid11723173.denovo.063"
                     /strain="LGE2415"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8302 N 95.9628 W"
                     /collection_date="2015-08-24"
     gene            complement(<1..233)
                     /locus_tag="J1Y83_002193"
     CDS             complement(<1..233)
                     /locus_tag="J1Y83_002193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HBA7630340.1"
                     /translation="MSKEKFERTKPHVNVGTIGHVDHGKTTLTAAITTVLAKTYGGAA
                     RAFDQIDNAPEEKARGITINTSHVEYDTPTRHYA"
     gene            complement(304..2418)
                     /gene="fusA"
                     /locus_tag="J1Y83_002194"
     CDS             complement(304..2418)
                     /gene="fusA"
                     /locus_tag="J1Y83_002194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020455498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HBA7630341.1"
                     /translation="MARTTPIARYRNIGISAHIDAGKTTTTERILFYTGVNHKIGEVH
                     DGAATMDWMEQEQERGITITSAATTAFWSGMAKQYEPHRINIIDTPGHVDFTIEVERS
                     MRVLDGAVMVYCAVGGVQPQSETVWRQANKYKVPRIAFVNKMDRMGANFLKVVNQIKT
                     RLGANPVPLQLAIGAEEHFTGVVDLVKMKAINWNDADQGVTFEYEDIPADMVELANEW
                     HQNLIESAAEASEELMEKYLGGEELSEAEIKGALRQRVLNNEIILVTCGSAFKNKGVQ
                     AMLDAVIDYLPSPVDVPAINGILDDGKDTPAERHASDDEPFSALAFKIATDPFVGNLT
                     FFRVYSGVVNSGDTVLNSVKAARERFGRIVQMHANKREEIKEVRAGDIAAAIGLKDVT
                     TGDTLCDPDAPIILERMEFPEPVISIAVEPKTKADQEKMGLALGRLAKEDPSFRVWTD
                     EESNQTIIAGMGELHLDIIVDRMKREFNVEANVGKPQVAYRETIRQKVTDVEGKHAKQ
                     SGGRGQYGHVVIDMYPLEPGSNPKGYEFINDIKGGVIPGEYIPAVDKGIQEQLKAGPL
                     AGYPVVDMGIRLHFGSYHDVDSSELAFKLAASIAFKEGFKKAKPVLLEPIMKVEVETP
                     EENTGDVIGDLSRRRGMLKGQESEVTGVKIHAEVPLSEMFGYATQLRSLTKGRASYTM
                     EFLKYDEAPSNVAQAVIEARGK"
     gene            complement(2515..2985)
                     /gene="rpsG"
                     /locus_tag="J1Y83_002195"
     CDS             complement(2515..2985)
                     /gene="rpsG"
                     /locus_tag="J1Y83_002195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006714698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HBA7630342.1"
                     /translation="MPRRRVIGQRKILPDPKFGSELLAKFVNILMVDGKKSTAESIVY
                     SALETLAQRSGKSELEAFEVALENVRPTVEVKSRRVGGSTYQVPVEVRPVRRNALAMR
                     WIVEAARKRGDKSMALRLANELSDAAENKGTAVKKREDVHRMAEANKAFAHYRW"
     gene            complement(3082..3456)
                     /gene="rpsL"
                     /locus_tag="J1Y83_002196"
     CDS             complement(3082..3456)
                     /gene="rpsL"
                     /locus_tag="J1Y83_002196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003852912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HBA7630343.1"
                     /translation="MATVNQLVRKPRARKVAKSNVPALEACPQKRGVCTRVYTTTPKK
                     PNSALRKVCRVRLTNGFEVTSYIGGEGHNLQEHSVILIRGGRVKDLPGVRYHTVRGAL
                     DCSGVKDRKQARSKYGVKRPKA"
     gene            complement(3582..3869)
                     /gene="tusB"
                     /locus_tag="J1Y83_002197"
     CDS             complement(3582..3869)
                     /gene="tusB"
                     /locus_tag="J1Y83_002197"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusB"
                     /protein_id="HBA7630344.1"
                     /translation="MLHTLHRSPWLTDFAALLRLLSEGDELLLLQDGVTAAVDGNRYL
                     ESLRNAPIKVYALNEDLIARGLTGRISNDIISIDYTDFVRLTVKHSSQMAW"
     gene            complement(3876..4235)
                     /gene="tusC"
                     /locus_tag="J1Y83_002198"
     CDS             complement(3876..4235)
                     /gene="tusC"
                     /locus_tag="J1Y83_002198"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusC"
                     /protein_id="HBA7630345.1"
                     /translation="MKRIAFVFSTAPHGTTAGREGLDALLATSALTDDLAVFFIADGV
                     FQLLPGQKPDAVLARDYIATFKLLGLYDIEQCWICAASLRERGLDPQTPFVVEATPLE
                     ADALRRELANYDVILRF"
     gene            complement(4235..4621)
                     /gene="tusD"
                     /locus_tag="J1Y83_002199"
     CDS             complement(4235..4621)
                     /gene="tusD"
                     /locus_tag="J1Y83_002199"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusD"
                     /protein_id="HBA7630346.1"
                     /translation="MRFAIVVTGPAYGTQQASSAFQFAQALIAEGHELSSVFFYREGV
                     YNANQLTSPASDEFDLVRGWQQLNAQHGVALNICVAAALRRGVVDETEAGRLGLASSN
                     LQPGFTLSGLGALAEASLTCDRVVQF"
     gene            complement(4621..5343)
                     /locus_tag="J1Y83_002200"
     CDS             complement(4621..5343)
                     /locus_tag="J1Y83_002200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HBA7630347.1"
                     /translation="MSRSLLTNETSELDLLDQRPFDQTDFDILKSYEAVVDGLAMLIG
                     SHCEIVLHSLQDLKCSAIRIANGEHTGRKIGSPITDLALRMLHDMTGADSSVSKCYFT
                     RAKSGVLMKSLTIAIRNREQRVIGLLCINMNLDVPFSQIMSTFVPPETPDVGSSVNFA
                     SSVEDLVTQTLEFTIEEVNADRNVSNNAKNRQIVLNLYEKGIFDIKDAINQVADRLNI
                     SKHTVYLYIRQFKSGDFQGQDK"
     gene            complement(5510..6322)
                     /gene="fkpA"
                     /locus_tag="J1Y83_002201"
     CDS             complement(5510..6322)
                     /gene="fkpA"
                     /locus_tag="J1Y83_002201"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003827768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HBA7630348.1"
                     /translation="MKSLFKVTLLATTMAVALHAPITFAAEAAKPATTADSKAAFKND
                     DQKSAYALGASLGRYMENSLKEQEKLGIKLDKDQLIAGVQDAFADKSKLSDQEIEQTL
                     QAFEARVKSSAQAKMEKDAADNEAKGKEYREKFAKEKGVKTSSTGLVYQVVEAGKGEA
                     PKDSDTVVVNYKGTLIDGKEFDNSYTRGEPLSFRLDGVIPGWTEGLKNIKKGGKIKLV
                     IPPELAYGKAGVPGIPPNSTLVFDVELLDVKPAPKADAKPEADAKAADSAKK"
     gene            6543..6761
                     /locus_tag="J1Y83_002202"
     CDS             6543..6761
                     /locus_tag="J1Y83_002202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein SlyX"
                     /protein_id="HBA7630349.1"
                     /translation="MQDLSLEARLAELESRLAFQEITIEELNVTVTAHEMEMAKLRDH
                     LRLLTEKLKASQPSNIASQAEETPPPHY"
     gene            complement(6811..7401)
                     /gene="slyD"
                     /locus_tag="J1Y83_002203"
     CDS             complement(6811..7401)
                     /gene="slyD"
                     /locus_tag="J1Y83_002203"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HBA7630350.1"
                     /translation="MKVAKDLVVSLAYQVRTEDGVLVDESPVSAPLDYLHGHGSLISG
                     LETALEGHEVGDKFDVAVGANDAYGQYDENLVQRVPKDVFMGVDELQVGMRFLAETDQ
                     GPVPVEITAVEDDHVVVDGNHMLAGQNLKFNVEVVAIREATEEELAHGHVHGAHDHHH
                     DHDHDGCCGGHGHDHGHEHGGEGCCGGKGNGGCGCH"
     gene            complement(7496..7696)
                     /locus_tag="J1Y83_002204"
     CDS             complement(7496..7696)
                     /locus_tag="J1Y83_002204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YheV family putative metal-binding protein"
                     /protein_id="HBA7630351.1"
                     /translation="MAIRKRFIAGAKCPACQAQDSMAMWRENNIDIVECVKCGHQMRE
                     ADKEARDHVRKDEQVIGIFHPD"
     gene            complement(7706..9511)
                     /gene="kefB"
                     /locus_tag="J1Y83_002205"
     CDS             complement(7706..9511)
                     /gene="kefB"
                     /locus_tag="J1Y83_002205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     protein KefB"
                     /protein_id="HBA7630352.1"
                     /translation="MEGSDFLLAGVLFLFAAVAAVPLASRLGIGAVLGYLLAGIAIGP
                     WGLGFISDVDEILHFSELGVVFLMFIIGLELNPSKLWQLRRSIFGVGAAQVLLSAALL
                     AGLLMLTHFSWQAAVVGGIGLAMSSTAMALQLMREKGMNRSESGQLGFSVLLFQDLAV
                     IPALALVPLLAGSADEHFDWMKIGMKVLAFVGMLIGGRYLLRPVFRFIAASGVREVFT
                     AATLLLVLGSALFMDALGLSMALGTFIAGVLLAESEYRHELETAIDPFKGLLLGLFFI
                     SVGMSLNLGVLYTHLLWVVISVVVLVAVKILVLYLLARLYGVRSSERMQFAGVLSQGG
                     EFAFVLFSTASSQRLFQGDQMALLLVTVTLSMMTTPLLMKLVDKWLSRQFNGPEEEDE
                     KPWVNDDKPQVIVVGFGRFGQVIGRLLMANKMRITVLERDISAVNLMRKYGYKVYYGD
                     ATQVDLLRSAGAEAAESIVITCNEPEDTMKLVEICQQHFPHLHILARARGRVEAHELL
                     QAGVTQFSRETFSSALELGRKTLVTLGMHPHQAQRAQLHFRRLDMRMLRELIPMHADT
                     VQISRAREARRELEEIFQREMQQERRQLDGWDEFE"
     gene            complement(9511..10062)
                     /gene="kefG"
                     /locus_tag="J1Y83_002206"
     CDS             complement(9511..10062)
                     /gene="kefG"
                     /locus_tag="J1Y83_002206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312229.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     ancillary protein KefG"
                     /protein_id="HBA7630353.1"
                     /translation="MSQPAKVLLLYAHPESQDSVANRVLLKPATQLSNVTVHDIYAHY
                     PDFFIDIPREQALLREHEVIVFQHPLYTYSCPALLKEWLDRVLSRGFASGPGGNQLAG
                     KYWRSVITTGEPESAYRYDALNRYPMSDVLRPFELAAGMCRMHWLSPIIIYWARRQSA
                     QELASHARAYGDWLANPLSPGGR"
     gene            10193..12106
                     /locus_tag="J1Y83_002207"
     CDS             10193..12106
                     /locus_tag="J1Y83_002207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBA7630354.1"
                     /translation="MIVFSSLQIRRGVRVLLDNATATINPGQKVGLVGKNGCGKSTLL
                     ALLKNEISADGGSYTFPGSWQLAWVNQETPALPQAALEYVIDGDREYRQLEAQLHDAN
                     ERNDGHAIATIHGKLDAIDAWSIRSRAASLLHGLGFSNEQLERPVSDFSGGWRMRLNL
                     AQALICRSDLLLLDEPTNHLDLDAVIWLEKWLKSYQGTLILISHDRDFLDPIVDKIIH
                     IEQQSMFEYTGNYSSFEVQRATRLAQQQAMYESQQERVAHLQSYIDRFRAKATKAKQA
                     QSRIKMLERMELIAPAHVDNPFRFSFRAPESLPNPLLKMEKVSAGYGDRIILDSIKLN
                     LVPGSRIGLLGRNGAGKSTLIKLLAGELAPVSGEIGLAKGIKLGYFAQHQLEYLRADE
                     SPIQHLARLAPQELEQKLRDYLGGFGFQGDKVTEETRRFSGGEKARLVLALIVWQRPN
                     LLLLDEPTNHLDLDMRQALTEALIEFEGALVVVSHDRHLLRSTTDDLYLVHDRKVEPF
                     DGDLEDYQQWLSDVQKQENQTDEAPKENANSAQARKDQKRREAELRAQTQPLRKEIAR
                     LEKEMEKLNAQLAQAEEKLGDSELYDQSRKAELTACLQQQASAKSGLEECEMAWLEAQ
                     EQLEQMLLEGQSN"
     gene            12106..13128
                     /locus_tag="J1Y83_002208"
     CDS             12106..13128
                     /locus_tag="J1Y83_002208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HBA7630355.1"
                     /translation="MAQITTTDANEFSSSAKFTPMRGFSNCHLQTMLPRLFRRKVKFT
                     PYWQRLELPDGDFVDLAWSEDPAQARHKPRLVVFHGLEGSLNSPYAHGLVEAAQKRGW
                     LGVVMHFRGCSGEPNRMHRIYHSGETEDASWFLRWLQREFGHAPTAAVGYSLGGNMLA
                     CLLAKEGNDLPIDAAVIVSAPFMLEACSYHMEKGFSRVYQRYLLNLLKANAARKLAAY
                     PGTLPINLAQLKSVRRIREFDDLITARIHGYADAIDYYRQCSAMPMLNRIAKPTLIIH
                     AKDDPFMDHQVIPKPESLPPQVEYQLTEHGGHVGFIGGTLLHPQMWLESRIPDWLTTY
                     LEAKSC"
     gene            13122..13340
                     /locus_tag="J1Y83_002209"
     CDS             13122..13340
                     /locus_tag="J1Y83_002209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YheU family protein"
                     /protein_id="HBA7630356.1"
                     /translation="MLIPWQDLSPETLENLIESFVLREGTDYGEHERTLEQKVADVKR
                     QLQCGEAVLVWSELHETVNIMPRSQFRE"
     gene            13394..14263
                     /locus_tag="J1Y83_002210"
     CDS             13394..14263
                     /locus_tag="J1Y83_002210"
                     /EC_number="2.7.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribulokinase"
                     /protein_id="HBA7630357.1"
                     /translation="MSAKHPVIAVTGSSGAGTTTTSLAFRKIFAQLNLHAAEVEGDSF
                     HRYTRPEMDMAIRKARDAGRHISYFGPEANDFGLLEQTFIEYGQSGKGKSRKYLHTYD
                     EAVPWNQVPGTFTPWQSLPEPTDVLFYEGLHGGVVTPQHNVAQHVDLLVGVVPIVNLE
                     WIQKLIRDTSERGHSREAVMDSVVRSMEDYINYITPQFSRTHLNFQRVPTVDTSNPFA
                     AKGIPSLDESFVVIHFRNLEGIDFPWLLAMLQGSFISHINTLVVPGGKMGLAMELIML
                     PLVQRLMEGKKIE"
     gene            complement(14318..14722)
                     /locus_tag="J1Y83_002211"
     CDS             complement(14318..14722)
                     /locus_tag="J1Y83_002211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HBA7630358.1"
                     /translation="MQARVKWVEGLTFLGESASGHQILMDGNSGDKAPSPMEMVLMAA
                     GGCSAIDVVSILQKGRQDVVDCEVKLTSERREEAPRLFTHINLHFIVTGRDLKDAAVA
                     RAVDLSAEKYCSVALMLEKAVNITHSYEVVAA"
     gene            15024..15656
                     /gene="crp"
                     /locus_tag="J1Y83_002212"
     CDS             15024..15656
                     /gene="crp"
                     /locus_tag="J1Y83_002212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cAMP-activated global transcriptional regulator
                     CRP"
                     /protein_id="HBA7630359.1"
                     /translation="MVLGKPQTDPTLEWFLSHCHIHKYPSKSTLIHQGEKAETLYYIV
                     KGSVAVLIKDEEGKEMILSYLNQGDFIGELGLFEEGQERSAWVRAKTACEVAEISYKK
                     FRQLIQVNPDILMRLSAQMARRLQVTSEKVGNLAFLDVTGRIAQTLLNLAKQPDAMTH
                     PDGMQIKITRQEIGQIVGCSRETVGRILKMLEDQNLISAHGKTIVVYGTR"
     gene            15707..17797
                     /locus_tag="J1Y83_002213"
     CDS             15707..17797
                     /locus_tag="J1Y83_002213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312236.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA7630360.1"
                     /translation="MWRRLIYHPDINYALRQTLVLCLPVAVGLMLGELRFGLLFSLVP
                     ACCNIAGLDTPHKRFFKRLIIGASLFATCSLLTQVLLAKDVPLPFLLTGLTLVLGVTA
                     ELGPLHAKLLPASLLAAIFTLSLAGYMPVWEPLLIYALGTLWYGLFNWFWFWIWREQP
                     LRESLSLLYRELADYCEAKYSLLTQHTDPEKALPPLLVRQQKAVDLITQCYQQMHMLS
                     AQNNTDYKRMLRIFQEALDLQEHISVSLHQPEEVQKLVERSHAEEVIRWNAQTVAARL
                     RVLADDILYHRLPTRFTMEKQIGALEKIARQHPDNPVGQFCYWHFSRIARVLRTQKPL
                     YARDLLADKQRRMPLLPALKSYLSLKSPALRNAGRLSVMLSVASLMGTALHLPKSYWI
                     LMTVLLVTQNGYGATRLRIVNRSVGTVVGLIIAGVTLHFKIPEGYTLTLMLITTLASY
                     LILRKNYGWATVGFTITAVYTLQLLWLNGEQYILPRLIDTIIGCLIAFGGTVWLWPQW
                     QSGLLRKNAHDALEAYQDAIRLILSEDPQPTPLAWQRMRVNQAHNTLYNSLNQAMQEP
                     AFNSHYLADMKLWVTHSQFIVEHINAMTTLAREHRALPPELAQEYLQSCEIAIQRCQQ
                     RLEYDEPGSSGDANIMDAPEMQPHEGAAGTLEQHLQRVIGHLNTMHTISSMAWRQRPH
                     HGIWLSRKLRDSKA"
     gene            complement(17864..19084)
                     /gene="argD/dapC"
                     /locus_tag="J1Y83_002214"
     CDS             complement(17864..19084)
                     /gene="argD/dapC"
                     /locus_tag="J1Y83_002214"
                     /EC_number="2.6.1.11"
                     /EC_number="2.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     acetylornithine/succinyldiaminopimelate transaminase"
                     /protein_id="HBA7630361.1"
                     /translation="MAIEQTAITRATFDEVILPIYAPAEFIPVKGQGSRIWDQQGKEY
                     VDFAGGIAVTALGHCHPALVNALKTQGETLWHISNVFTNEPALRLGRKLIEATFAERV
                     VFMNSGTEANETAFKLARHYACVRHSPFKTKIIAFHNAFHGRSLFTVSVGGQPKYSDG
                     FGPKPADIIHVPFNDLHAVKAVMDDHTCAVVVEPIQGEGGVTAATPEFLQGLRELCDQ
                     HQALLVFDEVQSGMGRTGDLFAYMHYGVTPDILTSAKALGGGFPISAMLTTAEIASAF
                     HPGSHGSTYGGNPLACAVAGAAFDIINTPEVLEGIQAKRQRFVDHLQKIDQQYDVFSD
                     IRGMGLLIGAELKPQYKGRARDFLYAGAEEGAMVLNAGPDVMRFAPSLVVEDADIDEG
                     MHRFAHAVAKVVGA"
     gene            complement(19170..19733)
                     /gene="pabA"
                     /locus_tag="J1Y83_002215"
     CDS             complement(19170..19733)
                     /gene="pabA"
                     /locus_tag="J1Y83_002215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component 2"
                     /protein_id="HBA7630362.1"
                     /translation="MILLIDNYDSFTWNLYQYFCELGADVLVKRNDALTLADIDALKP
                     QKIVISPGPCTPDEAGISLDVIRHYAGRLPILGVCLGHQAMAQAFGGKVVRAAKVMHG
                     KTSPITHNGVGVFKGLANPLTVTRYHSLVVEPDSLPECFEVTAWSETREIMGIRHRQW
                     DLEGVQFHPESILSEQGHQLLANFLHR"
     gene            complement(19765..20367)
                     /locus_tag="J1Y83_002216"
     CDS             complement(19765..20367)
                     /locus_tag="J1Y83_002216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative adenosine monophosphate-protein
                     transferase Fic"
                     /protein_id="HBA7630363.1"
                     /translation="MSDKFGEGRDPYLYPGLDIMRNRLNIRQQQRLEQAAYEMTALRA
                     ATIELGPLVRGLPHLRTLHRQLYQDIFDWAGQLREVDIYQGDTPFCHFAYIEKEGNAL
                     MQDLEEEGYLVGLEKAKFVERLAHYYCEINVLHPFRVGSGLAQRIFFEQLAIHAGYQL
                     SWQGIEKEAWNQANQNGAMGDLTALQMIFSKVVSEAGESE"
     gene            complement(20357..20524)
                     /locus_tag="J1Y83_002217"
     CDS             complement(20357..20524)
                     /locus_tag="J1Y83_002217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfG family protein"
                     /protein_id="HBA7630364.1"
                     /translation="MKKLTDKQKSRLWELQRNRNFQASRRLEGVEMPLVTLTAAEALA
                     RLEALRRHYER"
     gene            complement(20629..21201)
                     /gene="ppiA"
                     /locus_tag="J1Y83_002218"
                     /gene_synonym="rotA"
     CDS             complement(20629..21201)
                     /gene="ppiA"
                     /locus_tag="J1Y83_002218"
                     /gene_synonym="rotA"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000920493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase A"
                     /protein_id="HBA7630365.1"
                     /translation="MFKSTLAAMAAVFALSALSPAAMAAKGDPHVLLTTSAGNIELEL
                     DKQKAPVSVQNFVDYVNSGFYNNTTFHRVIPGFMIQGGGFTEQMQQKKPNPPIKNEAD
                     NGLRNTRGTIAMARTADKDSATSQFFINVADNAFLDHGQRDFGYAVFGKVVKGMDVAD
                     KISQVPTHDVGPYQNVPSKPVVILSAKVLP"
     gene            21471..22652
                     /gene="tsgA"
                     /locus_tag="J1Y83_002219"
     CDS             21471..22652
                     /gene="tsgA"
                     /locus_tag="J1Y83_002219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter TsgA"
                     /protein_id="HBA7630366.1"
                     /translation="MTNSNRIKLTWISFLSYALTGALVIVTGMVMGNIADYFNLPVSS
                     MSNTFTFLNAGILISIFLNAWLMEIVPLKTQLRFGFLLMVLAVAGLMFSHSLALFSAA
                     MFILGVVSGITMSIGTFLVTQMYEGRQRGSRLLFTDSFFSMAGMIFPMIAAFLLARSI
                     EWYWVYAGIGLVYVAIFILTFGCEFPALGKHAPKTDAPVEKEKWGIGVLFLSVAALCY
                     ILGQLGFISWVPEYAKGLGMSLNDAGTLVSNFWMSYMVGMWAFSFILRFFDLQRILTV
                     LAGLAAILMYVFNTGTPAHMAWSILALGFFSSAIYTTIITLGSQQTKVPSPKLVNFVL
                     TCGTIGTMLTFVVTGPIVEHSGPQAALLTANGLYAVVFVMCFLLGFVSRHRQHNTLTS
                     H"
     gene            22914..25457
                     /locus_tag="J1Y83_002220"
     CDS             22914..25457
                     /locus_tag="J1Y83_002220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite reductase large subunit"
                     /protein_id="HBA7630367.1"
                     /translation="MSKVRLAIIGNGMVGHRFIEDLLDKSDAANFDITVFCEEPRIAY
                     DRVHLSSYFSHHTAEELSLVREGFYEKHGIKVLVGERAITINRQEKVIHSSAGRTVFY
                     DKLIMATGSYPWIPPIKGSDTQDCFVYRTIEDLNAIESCARRSKRGAVVGGGLLGLEA
                     AGALKNLGIETHVIEFAPMLMAEQLDQMGGEQLRRKIESMGVRVHTSKNTLEIVQEGV
                     EARKTMRFADGSELEVDFIVFSTGIRPRDKLATQCGLDVAPRGGIVINDSCQTSDPDI
                     YAIGECASWNNRVFGLVAPGYKMAQVAVDHILGNENAFEGADLSAKLKLLGVDVGGIG
                     DAHGRTPGARSYVYLDESKEIYKRLIVSEDNKTLLGAVLVGDTSDYGNLLQLVLNAIE
                     LPENPDSLILPAHSGSGKPSIGVDKLPDSAQICSCFDVTKGDLIAAINKGCHTVAALK
                     AETKAGTGCGGCIPLVTQVLNAELAKQGIEVNNNLCEHFAYSRQELFHLIRVEGIKTF
                     EELLAKHGKGYGCEVCKPTVGSLLASCWNEYILKPEHTPLQDSNDNFLANIQKDGTYS
                     VIPRSPGGEITPEGLMAVGRIAREFNLYTKITGSQRLAMFGAQKDDLPEIWRQLIEAG
                     FETGHAYAKALRMAKTCVGSTWCRYGVGDSVGLGVELENRYKGIRTPHKMKFGVSGCT
                     RECSEAQGKDVGIIATEKGWNLYVCGNGGMKPRHADLLAADIDRETLIKYLDRFMMFY
                     IRTADKLTRTAPWLENLEGGIDYLKAVIIDDKLGLNAHLEEEMARLREAVVCEWTETV
                     NTPSAQTRFKHFINSDKRDPNVQMVPEREQHRPATPYERIPVTLVEDNA"
     gene            25454..25780
                     /gene="nirD"
                     /locus_tag="J1Y83_002221"
     CDS             25454..25780
                     /gene="nirD"
                     /locus_tag="J1Y83_002221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite reductase small subunit NirD"
                     /protein_id="HBA7630368.1"
                     /translation="MSQWKDICKIDDILPETGVCALLGDEQVAIFRPYHSDQVFAISN
                     IDPFFESSVLSRGLIAEHQGELWVASPLKKQRFRLSDGLCMEDEQFSVKHYDARVKDG
                     VVQLRG"
BASE COUNT         6294 a         6971 c         6478 g         6063 t
ORIGIN      
        1 gcgtagtgac gggtcggggt gtcgtattca acgtgagaag tgttgatggt gataccacga
       61 gctttttctt ccggcgcgtt atcgatctgg tcgaatgcac gagcagcacc gccgtaggtt
      121 ttagccagta cggtggtgat tgcagcggtc agagtagttt taccgtggtc aacgtggccg
      181 atagtaccaa cgttaacgtg cggttttgta cgttcaaatt tttctttaga cacggctata
      241 ttccttacta tagtgctctc cccttcagga gagagcacgg gactttggta ttaaccctta
      301 ggcttattta ccacgggctt caattacggc ctgagcaacg ttactcggcg cttcatcata
      361 cttcaggaat tccatagtgt atgatgcgcg acctttggtc agagaacgca gctgagttgc
      421 atatccgaac atttcagaca gcggtacttc agcgtggatc ttaacgccag taacttcaga
      481 ttcctgacct ttgagcatac cacgacgacg gctcaagtca ccgataacgt caccggtgtt
      541 ctcttcagga gtttctactt caaccttcat gatcggctca agcagaactg gtttcgcttt
      601 cttaaagcct tctttaaagg cgatagaagc agccagttta aacgccagtt cagaggagtc
      661 aacgtcatgg taagaaccga agtgcagacg aatacccatg tctactaccg ggtagcctgc
      721 cagcggacct gctttcagct gttcctggat acctttatca acggccggga tgtattcgcc
      781 agggattaca ccacctttaa tgtcgttgat gaactcgtag cctttcgggt ttgaacccgg
      841 ctccagcggg tacatgtcga taacaacatg accatactga ccacgaccac cagactgttt
      901 cgcgtgttta ccttcaacat cggtaacttt ctggcggata gtttcacggt aagcaacctg
      961 cggtttacct acgttcgctt caacgttgaa ttcacgcttc atacggtcaa cgatgatgtc
     1021 gaggtgcagt tcgcccatac ccgcgatgat ggtctggtta gattcttcgt cagtccatac
     1081 acggaaagac gggtcttctt tagccagacg gcccagagcc agacccattt tttcctggtc
     1141 agctttggtt ttcggttcaa ctgcgatgga gattaccggc tcagggaatt ccatacgttc
     1201 cagaatgatc ggcgcatccg ggtcacacag ggtgtcacca gtggttacgt ctttcagacc
     1261 gattgcagct gcgatgtcgc ccgcgcgaac ttctttgatc tcttcacgtt tgttagcgtg
     1321 catctgaacg atacgaccga aacgctcacg tgcagctttc acggagttca gtacggtatc
     1381 accagagtta accacaccgg agtacacacg gaagaaggtc aggttaccaa caaacgggtc
     1441 ggtagcgatt ttgaacgcca gtgcagagaa cggctcgtca tcacttgcgt gacgctcagc
     1501 cggagtgtct ttaccgtcgt ccaggatacc gttgatcgca ggtacgtcaa ccggggatgg
     1561 caggtaatca attaccgcat ccagcatcgc ctgaacacct ttgttcttga acgcagaacc
     1621 acaggttacc aggatgattt cgttgttcag aacgcgctga cgcagagcac ctttgatttc
     1681 tgcttcactc agttcttcac cacccaggta tttttccatc agctcttcag aagcttcagc
     1741 tgcggattcg atcaggttct ggtgccattc gttagccagt tcaaccatgt ctgccgggat
     1801 atcttcgtat tcgaaggtta cgccctggtc agcgtcgttc cagttgatag ctttcatttt
     1861 caccaggtca acaacaccgg tgaaatgttc ttcagcacca atcgccagct gcagcggaac
     1921 cgggttcgcg cccagacggg ttttgatctg gttaacaact ttcaggaagt tcgcacccat
     1981 gcggtccatt ttgttaacga acgcaatacg cggaacttta tatttgtttg cctgacgcca
     2041 tacggtttca gactgcggct gaacaccacc aactgcgcag taaaccatta ccgcaccatc
     2101 gagaacacgc atggaacgtt ctacttcgat tgtgaagtca acgtgccccg gggtgtcgat
     2161 gatgttgatg cgatgcggct catactgctt agccatacca gaccagaatg cagtagtcgc
     2221 agcggaagtg atggtaatac cacgttcctg ctcctgctcc atccagtcca tggttgcagc
     2281 gccgtcatga acttcaccga ttttatggtt tacaccggtg tagaacagaa tacgttcggt
     2341 agtagtggtt ttaccggcgt cgatgtgcgc actgataccg atgttacggt agcgtgcgat
     2401 gggtgttgta cgagccattt gtttcctcgt ttatctttta ggcgttcaat ttaagtagcc
     2461 caaagcgggc tgcttactgg aagcgcccgc ctggtgacta aaactccgaa gggattacca
     2521 acggtagtgt gcgaacgcct tgttggcttc ggccatacgg tgaacgtctt cacgtttctt
     2581 aactgcagta cctttgtttt ctgcagcatc agaaagttcg ttcgccaggc gcagagccat
     2641 ggatttatca ccgcgtttac gagcagcttc aacgatccaa cgcattgcca gagcattacg
     2701 acgaaccgga cggacttcaa ctggtacctg ataagtagaa ccaccaacgc ggcgagactt
     2761 aacttctaca gtcgggcgca cgttttcgag agctacttcg aatgcttcca gttcagattt
     2821 accagagcgc tgagccaggg tctccagcgc gctgtatacg atagattcag cagtagattt
     2881 tttaccatct accatcagga tatttacaaa tttagccagc agttctgatc cgaacttcgg
     2941 atccggcaga attttacgct gaccaatgac gcgacgacgt ggcatggaaa tactccgttg
     3001 ttaattcagg attgtccaaa actctacgag tttagtttga catttaagtt aaaacgtttg
     3061 gccttactta acggagaacc attaagcctt aggacgcttc acgccatact tggaacgagc
     3121 ctgcttacgg tctttaacgc cggagcagtc aagcgcacca cgtacggtgt ggtaacgaac
     3181 acccgggagg tctttaacac gaccaccacg gatcaggatc acggagtgct cctgcaggtt
     3241 gtgaccttca ccaccgatgt aggaagtcac ttcgaaaccg ttagtcagac gaacacggca
     3301 tactttacgc agcgcggagt tcggtttttt aggagtggta gtatatacac gagtacatac
     3361 gccacgtttt tgcgggcatg cttccagcgc aggcacgttg cttttcgcaa ctttgcgagc
     3421 acgtggtttg cgtaccagct ggttaactgt tgccattaaa tagctcctgg ttttagcttt
     3481 tgcttcgtaa acacgtaata aaacgtcctc acacaatatg aggacgccga attttagggc
     3541 gatgccgaaa aggtgtcaag aaatatacaa cgatcccgcc atcaccaggc catctggctg
     3601 gagtgcttaa ccgtaagtct gacgaaatca gtatagtcaa tggaaatgat gtcgttcgaa
     3661 attcgaccag tcaaaccgcg ggcaataagg tcttcgttca gagcatagac cttaatgggg
     3721 gcattacgca gactttcaag gtagcggttg ccgtcaactg cggcagttac gccatcttgc
     3781 aataatagca gttcgtcacc ttcactgagc agacgcagca gcgcagcaaa gtccgtcagc
     3841 cagggtgaac gatgtaatgt gtgcagcata acgcctcaaa acctcaaaat aacatcgtag
     3901 ttggcgagtt cgcggcgtaa ggcatctgct tcgagcggcg tggcttcgac cacaaagggt
     3961 gtctgcggat ctaacccacg ttcgcgcagt gaagccgcgc aaatccagca ctgttcaata
     4021 tcgtacagac ccaacaattt aaaagtggca atgtaatcac gcgccagcac tgcatcgggc
     4081 ttttgtcctg gcagcagctg aaaaacgcca tcagcaataa agaagacggc cagatcgtca
     4141 gttaatgcgg aagttgccag taaagcatct aaaccttccc ggcctgcagt tgtaccatga
     4201 ggcgcagtag aaaaaacaaa cgcaattcgt ttcatcagaa ctgtaccacc ctgtcacagg
     4261 tcagcgaggc ttccgccagc gcgccaagtc cgcttaaggt aaatcccggc tgaaggtttg
     4321 acgaagcgag ccccagtctt ccggcctccg tttcatcaac aacgccacgg cgtaatgctg
     4381 ccgctacgca gatattcagc gccacaccat gttgcgcatt caactgttgc cagccgcgca
     4441 caaggtcaaa ttcgtcgctt gccggtgagg tcaattggtt agcgttatag accccttccc
     4501 gatagaaaaa aacgctgctt aactcatggc cttctgctat cagcgcctgc gcaaactgaa
     4561 aagcactact cgcctgttgc gtaccgtatg ctggcccggt caccacgatg gcaaaacgca
     4621 ttacttatct tgcccctgga aatcaccgct cttgaactgg cggatgtaga gatagacagt
     4681 gtgtttggag atgttcaggc ggtcagcaac ctggttgatc gcgtctttaa tatcgaagat
     4741 ccctttctcg tagagattta gcacgatctg acgatttttg gcgttattag aaacattgcg
     4801 atcggcattc acttcttcga tggtgaactc cagcgtttgg gtaaccagat cttcaacaga
     4861 agaggcaaag ttgacgcttg aaccgacatc cggagtttcc ggcggcacaa aagtgctcat
     4921 aatctgcgag aagggaacat caagattcat attgatgcac agcagaccaa ttacacgctg
     4981 ttcgcggtta cgaatcgcga tagtcaggga cttcattaat acgccgcttt tggcgcgagt
     5041 aaagtagcat ttagaaacgc tgctatccgc tcccgtcata tcgtgcagca tacgtagcgc
     5101 caggtcagta attggcgaac caatcttccg gcctgtatgt tctccgttag caatgcgaat
     5161 ggctgaacat tttagatcct gcaaagagtg caaaacgatt tcacagtggg agccaataag
     5221 catcgctaac ccgtccacca ccgcttcgta ggatttcaga atatcaaaat cggtctggtc
     5281 aaaaggacgt tgatccagta aatccaactc actggtttcg ttggttaaaa gcgacctgga
     5341 catgaaaaaa aacactcctt ttcaggagcc tgtcgttaac ttttcagggc aggctcatta
     5401 atgatgcggg taactaaatt aatacagcgg aggttccgct ttccagcact aattatatcc
     5461 ggcctgtaat aaaaaaaccg ccgcctggtc aggcggcggt tcttagtgct tattttttag
     5521 cagagtctgc ggctttcgca tcagcttccg gctttgcatc agccttcggt gctggtttca
     5581 catccagcag ctctacgtca aacaccaggg tagaattcgg tgggatcccc ggaacccccg
     5641 ctttgccgta agccagttct ggtggaataa ccagtttgat cttaccgcct ttcttgatgt
     5701 tcttcagacc ttctgtccaa cccgggataa caccgtccag acggaaagag agcggttcac
     5761 cacgagtgta agagttgtcg aactctttac cgtcgatcag cgtacctttg tagttcacga
     5821 cgacagtatc gctgtctttc ggggcttcgc ctttaccggc ttctactacc tgataaacca
     5881 gaccagtgga agaggttttc acacctttct ctttggcaaa tttctcgcgg tactctttac
     5941 cttttgcttc gttatcagcc gcgtcttttt ccatcttcgc ctgagcagaa gacttcacgc
     6001 gagcttcgaa tgcttgcaga gtctgttcga tctcttggtc ggaaagtttg ctcttatcag
     6061 caaatgcatc ctgaacacca gcgatcagct gatctttatc cagtttgatg cccagttttt
     6121 cttgttcttt aagagagttt tccatgtaac gacccagcga agcacccagt gcataagctg
     6181 atttctgatc gtcatttttg aacgctgctt tgctgtcagc agttgtagca ggttttgcag
     6241 cttcagcagc aaaagtgatt ggtgcatgca gggcaacggc cattgtggtc gccagcagcg
     6301 ttactttaaa cagtgatttc atccatatct ccaggatcgg ggcatctcac cccagggtta
     6361 actattatca gaagggtact ataaagcgtt gtcgaacaaa tctacataca gacacgccct
     6421 attatcatct attttcagac tctttttgtt taaattagtt tcgatgaccg cgaaatgagt
     6481 gctgtctcgg gcggcaaagt taagtagaat ccgcggcaac cattcgacaa aagaggtgaa
     6541 tcatgcagga tttatcattg gaagcacgcc tggcagagct ggagagccga ctggcttttc
     6601 aggaaatcac cattgaagaa ctgaacgtca cggtgaccgc tcatgaaatg gagatggcga
     6661 aactgcgcga tcatctgcgt ctgctgaccg agaagctaaa agccagccag ccgtcgaaca
     6721 tcgcgtcgca ggctgaagaa acgccaccgc cacattattg aggcgtaaaa aaagcgggga
     6781 ttcccccgct tttttgtcac tttttcggta ttagtggcaa ccgcaaccgc cgttaccttt
     6841 accgccgcag cagccttcgc caccgtgttc atgaccgtga tcgtggccat gaccgccgca
     6901 gcaaccgtcg tggtcgtgat cgtggtgatg atcgtgcgcg ccgtgaacgt ggccatgagc
     6961 cagttcttct tcggtcgctt cgcgaatcgc cacaacttca acgttgaatt tcaggttctg
     7021 accagccagc atgtggttac catcaaccac gacgtgatcg tcttcaaccg cagtgatttc
     7081 aaccggtacc ggaccctggt cggtttcagc caggaaacgc atacctacct gcagttcatc
     7141 aacgcccata aatacgtctt taggaacgcg ttgcaccagg ttttcgtcgt actgaccgta
     7201 agcgtcgttc gcgccaacag cgacatcaaa tttgtcgcca acttcgtgac cttccagcgc
     7261 cgtttccagg ccagagatca gggaaccgtg accatgcagg tagtccagcg gcgcactcac
     7321 cggagactca tcaaccaaca caccgtcttc tgtacgtacc tgataggcca ggctgaccac
     7381 caggtctttt gctactttca tgatatctcc tgagcatggg aagaatagtg gcgcagattg
     7441 tagcggaatt ctgcagccgt gtactcacta gcttaaaaaa actcggcgca tatcgctagt
     7501 ccggatgaaa aatcccgatc acttgctcat ctttgcggac gtgatcgcgg gcttctttgt
     7561 ctgcttctcg catctgatgt ccacacttaa cacattctac gatatcaata ttattttcgc
     7621 gccacatcgc cattgaatcc tgtgcctgac aggccgggca ttttgcgccc gcaataaaac
     7681 gttttcggat tgccatcttt accctctact caaattcatc ccagccgtcc agctggcgtc
     7741 gttcttgttg catttcacgc tggaaaatct cttccagttc acgtcgggct tccctggcgc
     7801 gagaaatttg tacggtatca gcatgcatcg ggattaactc ccgcagcatt cgcatatcca
     7861 ggcggcgaaa atgcagttgc gcgcgctgcg cctgatgcgg atgcatgcca agcgtgacca
     7921 gcgtcttgcg ccccagctct aacgcactgg agaatgtttc acgggaaaac tgcgtcaccc
     7981 ctgcctgtaa taactcatgc gcttccacac gtccgcgcgc tcgcgcaaga atatgcaaat
     8041 gcggaaagtg ctgctggcat atttccacca gcttcatagt gtcttccggc tcgttacagg
     8101 taatgacgat agactcagcg gcctctgcac ccgcagaacg taaaagatct acctgcgtgg
     8161 cgtcgccgta ataaactttg tagccatatt tgcgcatcag gttaacggca ctgatatccc
     8221 gctccagtac agtaatgcgc attttatttg ccatcagcaa acgaccaatc acctgaccaa
     8281 aacgcccaaa gcccacgaca atgacctgcg gtttatcatc gttgacccat ggtttttcgt
     8341 cttcttcttc cggtccgtta aactggcggg atagccattt atcgaccagc ttcatcagca
     8401 gcggcgtggt catcatggaa agcgtcaccg tcaccagcaa cagcgccatc tggtcgccct
     8461 ggaataagcg ttgtgaagaa gcggtagaaa agaggacaaa ggcaaactca ccgccctgac
     8521 tcaacacgcc agcaaactgc atccgctcag aactgcgcac gccatacaat cgcgccagca
     8581 gatacagcac gagaattttc accgccacca gcacaaccac acttatcact acccacaaca
     8641 gatgggtata aagcaccccg aggttgagcg acatgccgac agagataaag aacaaaccaa
     8701 gcagcaagcc tttgaaggga tcgatagccg tttccagttc atggcgatat tcactttccg
     8761 ccagtagcac acccgcaata aacgtaccga gcgccatcga caaccccagc gcatccataa
     8821 acaatgcgga acccaacacc agcagcagcg tcgcggcggt gaacacttcc cgcacaccag
     8881 aagctgcaat aaaacggaat accggacgca gtaaatagcg cccgccaatc agcatgccga
     8941 caaacgccag caccttcatg ccgatcttca tccagtcgaa atgttcgtct gccgaacccg
     9001 ccaacaacgg cactaacgcc agtgccggga tcaccgccag atcctgaaac agcagtaccg
     9061 agaaacctaa ctggccggat tcgctgcgat tcatcccttt ctcacgcatc aattgcaacg
     9121 ccattgcagt tgaagacatc gcaaggccaa tgccaccgac caccgccgcc tgccatgaga
     9181 aatgggtcag catcaataat cctgccagca acgccgcgct taacagcacc tgtgccgcgc
     9241 ctacgccaaa aatcgaacgc cgcagttgcc aaagcttgga gggattcaac tcaaggccga
     9301 tgataaacat caggaatacc acgccgagtt ccgaaaagtg gaggatctca tcgacgtcgc
     9361 taataaaccc cagcccccac gggccaatcg caatccccgc cagcaaatat cccaacactg
     9421 caccaatacc cagccgcgat gccagcggca ccgcagccac cgccgcgaag agaaacagca
     9481 ctcctgcgag taaaaaatca gaaccttcca tcagcggcct cctggagaca gcggatttgc
     9541 cagccagtca ccgtaggctc tggcgtggct cgccagctcc tgtgcgcttt gccgtctcgc
     9601 ccagtaaata atgatggggc ttaaccaatg catccggcac atgcccgccg ccagttcaaa
     9661 ggggcgcaga acatcgctca tcgggtagcg attcaacgcg tcataacggt aagcactttc
     9721 cggctccccg gtggtaatca cgctacgcca gtactttccc gccagttggt ttccccccgg
     9781 tccgctggca aaaccacggc ttaatacccg gtccagccac tctttcagta gcgccgggca
     9841 gctataggta taaagaggat gctgaaagac aatcacctcg tgctcgcgca gtaatgcctg
     9901 ctcacgaggg atatcaataa aaaagtcggg atagtgcgcg taaatgtcgt gcacggtaac
     9961 attgctgagc tgcgtggccg gtttaagcag aacccggttt gccaccgagt cctgagattc
    10021 cggatgggcg tacagcagca aaactttcgc tggctgagac atcatccccc tcccggtatg
    10081 gtttttgtgt atagtcgctg ttttgggcta ccattgcgcc cggtgcggca gctcgcccat
    10141 acattacatt atcataatga taagttaaca tagtctgaac atacggcgcc ttatgattgt
    10201 tttttcctcg ttacaaattc gtcgcggcgt gcgcgtcctg ctggataatg ccaccgccac
    10261 catcaaccct gggcagaaag tcggcctggt gggtaaaaac ggctgtggta aatctaccct
    10321 gctggcattg ctgaaaaatg aaatcagcgc cgacggcggc agctacacct ttccgggaag
    10381 ctggcaactg gcgtgggtga atcaggaaac accggcgtta ccgcaagcgg cgctggaata
    10441 tgtcattgac ggcgaccgtg aatatcgtca actggaagcg cagctacacg acgccaacga
    10501 acgtaacgac gggcacgcca ttgcgaccat tcatggcaag ctggatgcta ttgacgcatg
    10561 gagtattcgc tcccgtgccg ccagcctgct gcacggcctc ggtttcagca atgaacaact
    10621 ggagcgcccg gtaagtgatt tctccggtgg ctggcgtatg cgtcttaacc ttgcccaggc
    10681 gctgatttgc cgttcagact tgctgctgct cgacgaaccg actaaccacc tcgatctcga
    10741 tgccgttatc tggctggaaa aatggctgaa gagctatcag ggcacgctga tcctgatctc
    10801 tcacgaccgc gacttcctcg atccgatcgt tgataaaatt attcatatcg aacaacaaag
    10861 catgttcgag tacaccggca actacagttc gtttgaagta cagcgcgcca cccgtctggc
    10921 gcagcaacaa gcgatgtatg aaagccagca ggaacgcgtg gcgcatttgc aaagttatat
    10981 cgaccgtttc cgtgccaaag ccaccaaagc gaagcaggcc cagagccgca tcaagatgct
    11041 cgagcgtatg gagctgattg cccccgcgca cgttgacaac ccgtttcgtt ttagcttccg
    11101 agcgccggaa agcctgccta atccgttatt aaagatggaa aaagtcagcg caggctatgg
    11161 tgatcgcatt attctcgact cgattaaact gaatctggtc cccggctcgc gcattggtct
    11221 gttaggccgc aacggcgcgg gtaaatcgac attaatcaaa ctgttagccg gcgaacttgc
    11281 gccagtcagc ggtgaaattg gtctggcgaa agggatcaag ctcggctact tcgcccagca
    11341 tcaactggaa tacctgcgcg ccgacgaatc gccgattcaa catctggcac gtttagcgcc
    11401 gcaggagctg gagcaaaaac tgcgtgacta cctcggcggc tttggtttcc agggcgataa
    11461 agtaaccgaa gaaacgcgcc gcttctccgg tggggaaaaa gcccgcctgg tgctggcatt
    11521 aattgtctgg cagcgtccga atctgctgct gctcgacgaa ccgaccaacc accttgacct
    11581 cgacatgcgt caggcactca ccgaagcatt aatcgagttc gaaggcgcgc tggttgtcgt
    11641 ctcgcacgac cgtcatttgc tgcgttccac cactgacgat ctctacctgg ttcacgatcg
    11701 taaagtcgaa ccgttcgacg gcgatctgga agattatcaa cagtggctga gcgacgtaca
    11761 aaagcaggaa aaccagaccg acgaagcgcc aaaagagaac gcgaacagcg cccaggcacg
    11821 taaagatcag aagcgtcggg aagcggagct gcgtgcgcaa acccagccac tgcgtaaaga
    11881 gattgcccgt ctggaaaaag agatggagaa gctgaacgcg caactggcgc aggcggaaga
    11941 gaaactcggc gacagcgaac tgtatgacca gagccgtaaa gcggagttga ccgcctgcct
    12001 gcaacagcaa gccagcgcca aatccggcct ggaagagtgc gaaatggcat ggctggaagc
    12061 ccaggagcag cttgagcaga tgctgctgga aggccaaagc aactgatggc gcagataaca
    12121 acgaccgatg ccaatgaatt tagcagcagt gctaaattca cccctatgcg cggctttagc
    12181 aattgtcatc tacaaaccat gctgccgcgt ctgttccgtc gcaaggtgaa attcaccccg
    12241 tactggcagc ggctggagtt gcccgacggc gattttgtcg atctcgcgtg gagtgaagat
    12301 cctgcacagg cgcggcataa accgcgttta gtggtatttc acgggctgga aggcagtctc
    12361 aatagccctt acgctcacgg tctggttgag gcggcgcaaa agcgcggctg gctgggcgtg
    12421 gtgatgcatt ttcgcggatg cagcggtgaa ccaaaccgta tgcaccgcat ttaccattcg
    12481 ggcgaaaccg aagacgccag ttggttttta cgctggctgc aacgcgagtt tggtcatgcg
    12541 ccaacggctg ccgtcggcta ttcgctcggc ggtaatatgc tggcctgttt gctggcaaaa
    12601 gaaggcaatg atctcccgat tgatgcggca gtgattgtct ctgcgccgtt tatgctggag
    12661 gcctgtagtt accatatgga aaagggcttt tcccgcgttt atcagcgtta cttgctgaac
    12721 ctgttaaaag ccaatgccgc gcgcaagctg gcagcctacc ccggcacact gccgattaat
    12781 ctcgcgcagt taaaatcggt acgtcgcatc cgtgaatttg acgatctcat caccgccaga
    12841 attcacggct acgctgacgc tatcgactat tatcgtcagt gtagcgccat gccgatgctc
    12901 aatcggatag ccaaaccgac actgattatc cacgccaaag acgatccgtt tatggatcat
    12961 caggtgatcc cgaaaccgga aagtctcccc ccgcaggtgg agtatcagct gactgaacat
    13021 ggcggtcatg ttggctttat tggcggtaca ttacttcatc cgcaaatgtg gctggagtca
    13081 cgcattcctg actggttaac aacgtatctg gaggcgaaat catgttgatt ccgtggcaag
    13141 acctctcccc cgaaacgctg gaaaatttaa ttgaaagctt tgtgttacgt gaaggcaccg
    13201 attatggtga acatgagcgt acacttgaac agaaagtcgc cgacgtcaaa cgccagctac
    13261 agtgcggaga agcggtgctg gtatggtcgg agctgcacga aacggtcaat atcatgccgc
    13321 gcagtcagtt tcgcgaataa cctgcagccg tcggcggacg agataataat gatagctaag
    13381 tcatggagtt actatgtctg ccaaacatcc ggtcattgcg gtaacaggat ccagcggcgc
    13441 ggggaccacc accaccagtc tcgcgtttcg taaaatattc gcgcagttaa atctgcatgc
    13501 agctgaggtg gaaggcgaca gttttcaccg ttacacgcgc ccggaaatgg acatggcgat
    13561 ccgcaaggcg cgtgatgccg ggcggcatat cagctacttc ggccccgaag cgaacgactt
    13621 cggccttctg gaacaaacct tcattgaata cggtcagagc ggcaaaggga aatctcgcaa
    13681 atatctgcat acctacgatg aagccgtacc gtggaatcag gtaccgggga cattcacccc
    13741 ctggcaatct ttaccggaac ccactgatgt actgttttat gaaggtttac acggcggcgt
    13801 agtcacgcca cagcataacg ttgcgcagca tgtggactta ctggtcggcg tggtgcctat
    13861 cgttaacctt gagtggattc aaaaactgat ccgcgacacc agcgagcgcg ggcactcacg
    13921 agaagcagtg atggactcag tagtgcgctc aatggaagac tatatcaact acatcacacc
    13981 gcagttttcc cgcacccatc ttaacttcca gcgcgttccc accgtcgaca cctcaaaccc
    14041 gttcgcggca aaaggtatcc cgtcactgga tgaaagcttt gtggtgatcc attttcgtaa
    14101 tctggaaggg atcgatttcc cctggctgct ggcgatgttg caaggctcat tcatttccca
    14161 catcaatacg ttagtggtgc ctggcggcaa aatgggtctg gcaatggaat taattatgct
    14221 gccgctggtg caacgattga tggaaggaaa gaaaatcgag taacgctgct attacgccgg
    14281 ataaaatact atccggcttc acaacgggat agttaaatca cgcggcaacc acttcatacg
    14341 agtgagtaat attcaccgct ttttccagca tcagcgccac tgaacaatat ttctcggcag
    14401 aaagatcaac tgcacgcgca accgctgcgt ctttcaggtc gcgaccggtg acgataaaat
    14461 gcagattaat gtgcgtaaac aggcgtggtg cctcttcgcg gcgctcagag gtcaatttta
    14521 cttcacaatc gaccacatcc tgacgccctt tttgcaggat agaaaccaca tcgatggcac
    14581 tgcaaccacc cgccgccatc aacaccattt ccatcgggct tggtgcttta tcgcctgagt
    14641 tgccgtccat taaaatctgg tgaccagagg cggattcgcc cagaaaagtt aacccttcga
    14701 cccacttcac tcgcgcttgc atttttgcta ctccactgcg tcaattttcc tgacagagta
    14761 cgcgtactaa ccaaatcgcg caacggaagg cgacctgggt catgctgaag cgagacacca
    14821 ggagacacaa agcgaaagct atgctaaaac agtcaggatg ctacagtaat atattgatgt
    14881 actgcatgta tgcaaaggac gccacattac cgtgcagtac agttgatagc cccttcccag
    14941 gtagcgggaa gcatatttcg gcaatccaga gacagcggcg ttatctggct ctggagaaag
    15001 cttataacag aggataaccg cgcatggtgc ttggcaaacc gcaaacagac ccgactctcg
    15061 aatggttctt gtctcattgc cacattcata agtacccatc caagagcacg cttattcacc
    15121 agggtgaaaa agcggaaacg ctgtactaca tcgttaaagg ctctgtggca gtgctgatca
    15181 aagacgaaga gggtaaagaa atgatcctct cctatctgaa tcagggtgat tttattggcg
    15241 aactgggcct gtttgaagag ggccaggaac gtagcgcatg ggtacgtgcg aaaaccgcct
    15301 gtgaagtggc tgaaatttcg tacaaaaaat ttcgccaatt gattcaggta aacccggaca
    15361 ttctgatgcg tctgtctgca cagatggcgc gtcgtctgca agtcacttca gagaaagtgg
    15421 gcaacctggc gttcctcgac gtgacgggcc gcattgcaca gactctgctg aatctggcaa
    15481 aacaaccgga tgctatgact cacccggacg gtatgcaaat caaaattacc cgtcaggaaa
    15541 tcggtcagat tgtcggctgt tctcgtgaaa ccgtgggacg cattctgaag atgctggaag
    15601 atcagaacct gatctccgca cacggtaaaa ccatcgtcgt ttacggcact cgttaatccc
    15661 gtcagagtgg cgcgttacct ggtagcgcgc cattttgttt cccccgatgt ggcgcagact
    15721 gatttatcac cccgatatca actatgcact tcgacaaacg ctggtgctat gtttgcccgt
    15781 ggccgttggg ttaatgcttg gcgaattacg attcggcctg ctcttctccc tcgttcctgc
    15841 ctgttgcaat attgcgggcc ttgatacgcc tcataaacgt tttttcaaac gcttaatcat
    15901 tggtgcgtcg ctgtttgcca cctgtagctt gctgacacag gtgctactgg caaaagatgt
    15961 tcccctgccc tttttgctga ccggattaac gctggtactt ggcgtcactg ctgaactggg
    16021 gccattgcac gcaaaattgc ttcccgcatc gctgctcgct gccattttta ccctcagtct
    16081 ggcgggatac atgccggtct gggaaccgtt gctcatctat gcgctgggca ctttgtggta
    16141 cggattgttt aactggtttt ggttctggat ctggcgcgaa caaccgctac gcgagtcact
    16201 aagtctgctg taccgtgaac tggcagatta ctgtgaagcc aaatacagcc tgcttaccca
    16261 gcacaccgac cctgaaaaag cgctgccgcc gctgctggtg cgccagcaaa aagcggtcga
    16321 tctaattacc cagtgctatc agcaaatgca tatgctttcc gcgcaaaata ataccgatta
    16381 caagcggatg ctgcgtattt tccaggaggc gctggatttg caggaacata tttcggtcag
    16441 tttgcatcag ccggaagagg tgcaaaagct ggtcgagcgt agccatgcgg aagaagttat
    16501 ccgctggaat gcgcaaaccg tcgccgctcg cctgcgcgtg ctggctgatg acattcttta
    16561 ccatcgcctg ccaacgcgtt ttacgatgga aaagcaaatt ggcgcactgg aaaaaatcgc
    16621 ccgccaacac ccggataatc cggttgggca attctgctac tggcatttca gccgcattgc
    16681 tcgtgtgctg cgcacccaaa aaccgctcta tgcccgtgac ttactggccg ataaacagcg
    16741 gcgaatgcca ttacttccgg cgctgaaaag ttatctgtca ctaaagtctc cagcgctacg
    16801 caatgccgga cgactcagtg tgatgttaag cgttgccagc ctgatgggca ccgcgcttca
    16861 tctgccgaag tcgtactgga tcctgatgac agtattgctg gtgacgcaaa atggctatgg
    16921 cgcaacccgt ctgaggattg taaatcgctc cgtaggaacc gtggtcgggt taatcattgc
    16981 aggcgtgacg ctacacttta aaattcccga aggttacacc ctgaccttga tgctgattac
    17041 caccctcgcc agctacctga tattgcgcaa aaactacggc tgggcgacgg tcggttttac
    17101 tattaccgca gtgtataccc tgcaactgtt gtggctgaac ggtgagcagt acatccttcc
    17161 gcgtcttatc gataccatta ttggttgttt aattgctttc ggcggtactg tctggctgtg
    17221 gccgcagtgg cagagcgggt tattgcgtaa gaacgcccat gacgctttag aagcctatca
    17281 ggacgcgatt cgcttgattc tgagcgagga cccgcaacct acgccactgg cctggcagcg
    17341 aatgcgggtg aatcaggcgc ataacactct gtataactca ttgaatcagg cgatgcagga
    17401 accggcgttt aacagccatt atctggcaga tatgaaactg tgggtaacgc acagccagtt
    17461 cattgttgag catattaatg ccatgaccac gctggcgcgg gaacaccggg cattgccacc
    17521 ggaactggca caagagtatt tacagtcttg tgaaatcgcc attcagcgtt gtcagcagcg
    17581 actggagtat gacgaaccgg gtagttctgg cgatgccaat atcatggatg cgccggagat
    17641 gcagccgcac gaaggcgcgg caggtacgct ggagcagcat ttacaacggg ttattggtca
    17701 tctgaacacc atgcacacca tttcgtcgat ggcatggcgg cagcgaccgc atcacgggat
    17761 ttggctgagt cgcaagttgc gggattcgaa ggcgtaatac aggctaaacc atcggatgag
    17821 gtgttcacgc cgcatccgac atcttttgcc tgatgcgtct tatttacgcc ccaaccacct
    17881 tcgccaccgc gtgggcgaaa cgatgcatcc cttcatcgat atccgcatct tccaccacca
    17941 gcgacggcgc aaaacgcatc acatcaggcc cggcattcag caccattgcg ccttcctctg
    18001 cacccgcata caggaaatca cgcgcccgac ctttgtactg tggtttcagc tctgcgccaa
    18061 tcaacagccc cataccgcga atatcgctaa acacatcgta ctgctgatcg atcttctgca
    18121 aatggtcaac aaaacgctgg cgtttcgcct gaatgccttc cagcacttca ggggtattga
    18181 tgatatcaaa cgccgcgccc gctactgcac aggccagagg attaccgccg taggtggaac
    18241 cgtgagaacc aggatgaaac gcggaagcaa tctccgccgt ggtcagcatg gcgctaatcg
    18301 ggaaaccgcc acctaacgct ttcgcagagg tcagaatatc cggcgtcacg ccgtagtgca
    18361 tataggcaaa cagatcgccg gtccgcccca tcccggactg cacttcatca aacaccaata
    18421 atgcctgatg ctgatcacac agctcgcgca agccctgcaa aaactctggc gtcgctgccg
    18481 tcacgccgcc ctcgccctga atcggctcaa ccaccaccgc acaggtatgg tcatccatca
    18541 cggcttttac tgcgtgaaga tcgttaaaag gcacgtggat gatgtctgcc ggttttggcc
    18601 caaagccgtc ggaatatttt ggctgcccac ccaccgaaac ggtaaacagc gagcgaccat
    18661 gaaaagcgtt atggaaggca ataattttgg ttttgaacgg gctatgacgc acacaggcgt
    18721 aatggcgtgc cagtttaaag gcggtttcgt tagcttccgt gccggagttc ataaacacca
    18781 cgcgttcggc aaacgttgcc tcaatcagtt tacggccaag acgcagcgcc ggctcattgg
    18841 tgaaaacgtt actaatatgc cacagagttt cgccctgggt ttttaacgcg ttcaccagcg
    18901 caggatggca atggcccaac gctgtaactg caatgccacc cgcgaaatcg acatactcct
    18961 tgccctgttg atcccagatt cggctgccct gaccttttac cggaataaac tctgccggag
    19021 cataaatcgg cagaatcact tcatcgaaag tcgcgcgtgt aattgctgtt tgttcaattg
    19081 ccatctcatg atcaccctgt tacgcataaa taaatatgaa attataatca caaaatatgc
    19141 ataaaaaatc accgaatggc aatcagaaat cagcgatgca gaaaattagc cagcagttga
    19201 tgtccttgtt cgctaagaat actttctgga tggaattgca caccttccag atcccactgg
    19261 cgatggcgaa tccccataat ctcgcgggtt tcgctccagg ccgtcacttc aaagcactcc
    19321 ggtaatgagt caggttccac taccagcgaa tggtagcgtg tcacggtgag tggatttgcc
    19381 agccccttaa atacgcccac accattatgt gtaatcggcg aggttttgcc gtgcatgacc
    19441 tttgcggcgc gcacaacttt gccgccaaat gcctgcgcca ttgcctgatg accgaggcag
    19501 acgccaagaa tcggcaagcg tccggcatag tgacgaataa cgtcaaggga gatcccggct
    19561 tcatctggcg tacaggggcc gggtgagatg acaatttttt gtggtttaag ggcgtcgata
    19621 tccgccaacg tcaacgcatc gttgcgctta accaacacat ccgcccccag ttcacaaaag
    19681 tactggtaga ggttccaggt aaaagaatcg tagttatcaa taagcaggat catggcggct
    19741 ccggtacgaa agaaccgcgc tattttactc agattccccg gcttcgctta ccactttgct
    19801 aaatatcatc tgcagcgcgg tgagatcccc cattgcccca ttctgatttg cctgattcca
    19861 ggcctctttt tcaataccct gccagctcag ttgatatccg gcatgaatcg ccagttgctc
    19921 gaagaagatc cgctgtgcca aaccacttcc cacccggaag ggatgcagca cgttgatttc
    19981 acaatagtaa tgcgccagcc gctcgacgaa cttcgctttc tccaggccaa ccagataacc
    20041 ttcttcctcc agatcctgca tcagggcatt gccctctttt tcgatataag caaagtggca
    20101 gaacggcgta tcgccctgat aaatatcgac ttcgcgcagt tgccccgccc agtcgaaaat
    20161 atcctgatac aactggcgat gaagagtacg caaatgcggt aaaccccgaa ccaacgggcc
    20221 aagttcaatg gtcgcagcac gcagcgccgt catttcgtaa gcggcctgtt ccagccgctg
    20281 ctgctggcgg atgttcagcc ggttacgcat gatatcaagg cctggataaa gatacggatc
    20341 gcgcccttcg ccgaatttat cgctcatagt gcctcctcag cgcttcaagg cgcgctaaag
    20401 cctctgcggc agtaagagtg actaaaggca tctcgacgcc ttcaagacgg cgactggcct
    20461 ggaaattacg attacgctga agctcccaga gacgggactt ttgcttatcg gtgagtttct
    20521 tcacttgatg cctccctgaa cgtgtccatt tgccacaagt ataagcggca aatccgggtt
    20581 acgccgggag agcagaagcg cgggcaagat tgcccgcgcg agaaatcatt acggcaggac
    20641 tttagcggaa aggataacta ccggttttga cggcacattc tggtacggac caacgtcatg
    20701 agtcggcacc tgggaaatct tatcggcaac gtccatgcct ttcaccactt taccaaatac
    20761 cgcgtaaccg aaatcacgct gaccgtggtc aaggaaggcg ttatcggcaa cgttgataaa
    20821 gaactggctg gtggcgctgt ctttgtcagc ggtacgcgcc atcgcgatgg tgccacgcgt
    20881 gttgcgcagg ccgttatctg cttcattttt gattggcggg tttggttttt tctgctgcat
    20941 ctgctcggtg aaaccgccgc cctgaatcat aaagccagga atgacgcggt gaaaggtcgt
    21001 gttgttataa aagccactgt tcacataatc gacaaagttt tgcacagaca ctggcgcttt
    21061 ttgtttatcc agctccagtt cgatgttacc agctgaggtt gtcaacaata cgtgcgggtc
    21121 ccctttcgct gccattgctg cgggagaaag agcagaaaga gcgaaaacag cagccatcgc
    21181 cgccagggtc gatttgaaca tgagatttcc ttaacaaaca gagcagaaaa aaagcaagtg
    21241 aagagattct aaatagcctt caagaacaag gccatccctt tacctaattt tacgtatctg
    21301 aaatatatgt aacctgagga taacttttat tccgtgatca aaatcacctc ttaaaatgca
    21361 atttagcaac cgattgcaat aaaacattta atcagatcac aaaatcgcct aaaatcgccc
    21421 gctcgcggca tagcccgcta tgccatttta tttttgaacg caggccaatc atgactaaca
    21481 gcaatcgcat caagctcaca tggattagct ttctttccta cgcactgacc ggtgcgttgg
    21541 ttattgtcac cgggatggtg atgggaaata tcgccgatta tttcaatctg cctgtttcca
    21601 gcatgagtaa caccttcacc ttcctcaatg ccggcatttt aatctctatc ttcctcaacg
    21661 cctggctgat ggaaatcgtc ccgttgaaaa cgcagttacg ttttggcttt ctcctgatgg
    21721 tgctggcggt tgccggtttg atgttcagcc acagcctggc gctgttctcg gcggcgatgt
    21781 tcattctcgg ggtggtcagc ggcatcacca tgtcgattgg tacattcctg gtaacacaaa
    21841 tgtatgaagg acgtcagcgc ggttcacgcc tgttatttac cgactccttc ttcagtatgg
    21901 ccgggatgat tttcccaatg atcgccgcgt ttctgctggc acgtagcatt gagtggtact
    21961 gggtttatgc cggcatcggg ctggtgtatg ttgctatttt tattctgacc ttcggctgtg
    22021 aattcccggc actgggcaaa catgcgccaa aaacagatgc tccggtagag aaagaaaagt
    22081 gggggatcgg cgtactgttt ctctctgttg cggcgctgtg ctacatcctc ggtcagttag
    22141 gttttatctc ctgggtgccg gaatatgcca aaggcctggg catgagcctg aatgacgctg
    22201 gcacgctggt aagtaacttc tggatgtcgt acatggtcgg catgtgggcg ttcagcttta
    22261 ttcttcgctt ctttgatttg caacgtatcc tgaccgtact ggctggtctg gctgcgattc
    22321 tgatgtacgt ctttaacacc ggaacaccgg cacatatggc gtggtcaatt ctcgccctgg
    22381 gcttcttctc cagcgcgatc tataccacca tcatcaccct gggttcacag cagaccaaag
    22441 taccgtcgcc aaaactggtt aactttgtcc tgacctgcgg gaccatcggt actatgttga
    22501 cctttgtggt taccggcccg attgttgaac atagtggtcc gcaggcggcg ctgctgacgg
    22561 caaacggtct gtacgctgtc gtctttgtga tgtgcttcct gttaggtttc gtcagccgtc
    22621 accgtcagca taacaccctg acctctcatt aattgttcat gccgggcggc actatcgtcg
    22681 tccggccttt tcctctctta ctctgctacg tacatctatt tctataaatc cgttcaattt
    22741 gtctgttttt tgcacaaaca tgaaatgtca gacaattccg tgacttaaga aaatttatac
    22801 aaatcagcaa tatacccctt aaggagtata taaaggtgaa tttgatttac atcaataagc
    22861 ggggttgctg aatcgttaag gtaggcggta atagaaaaga aatcgaggca aaaatgagca
    22921 aagtcagact cgcaattatc ggtaacggta tggtcggcca tcgctttatc gaagatcttc
    22981 ttgataaatc tgatgcggcc aactttgata ttaccgtttt ctgtgaagaa ccgcgcatcg
    23041 cttatgaccg cgtacacctc tcgtcttact tctctcacca caccgccgaa gagctgtcgc
    23101 tggtgcgcga aggcttctac gagaaacacg gcatcaaagt tctggtcggc gaacgcgcta
    23161 tcaccatcaa ccgtcaggag aaggtgattc actccagcgc cggacgtacc gttttttatg
    23221 acaagctgat catggcgacc ggttcctacc cgtggatccc gccaattaaa ggttctgata
    23281 ctcaggactg ctttgtctat cgcaccattg aagacctcaa cgccattgaa tcctgcgctc
    23341 gtcgcagcaa gcgcggtgcc gttgttggtg gcggcctgtt aggtctggaa gctgctggcg
    23401 cgctgaaaaa cttaggcatt gaaacccacg ttatcgaatt tgcccctatg ctgatggcgg
    23461 aacagcttga tcagatgggt ggcgagcagc tgcgtcgcaa aatcgaaagt atgggcgtgc
    23521 gcgttcacac cagcaaaaac actcttgaga ttgtgcagga aggtgttgaa gcgcgtaaaa
    23581 ccatgcgttt tgccgacggc agcgaactgg aagtcgactt tatcgtcttc tctaccggta
    23641 tccgtccgcg cgataagctg gcaacccagt gtggtctgga cgttgctccg cgtgggggta
    23701 tcgtcatcaa tgattcctgc cagacttccg atccggatat ctacgccatc ggtgaatgcg
    23761 caagctggaa caaccgtgta tttggtctgg tagcacctgg ctacaaaatg gcgcaggtcg
    23821 ccgttgacca tattctcggt aacgaaaacg cctttgaagg tgctgacctt agcgccaagc
    23881 tgaaactgct gggcgtggac gtaggcggta ttggtgatgc gcacggtcgc acgcctggcg
    23941 cacgtagcta cgtttacctc gacgaaagca aagagatcta caaacgcctg attgtcagcg
    24001 aagacaacaa aaccctgctc ggtgcggtac tggtgggcga taccagcgac tacggtaact
    24061 tgctgcaact ggtgctgaac gctattgagc tgccggaaaa cccggattcc ctgatcctgc
    24121 cagcgcactc gggtagcggc aagccgtcta tcggtgttga taaactgccg gacagcgcgc
    24181 aaatctgctc ctgcttcgac gtcaccaaag gcgatctgat tgctgctatc aacaaaggct
    24241 gccacaccgt tgcggcgctg aaagccgaaa ccaaagccgg tacgggctgc ggtggctgta
    24301 tcccgctggt cactcaggta ctgaacgcgg aactggcgaa acagggcatc gaagttaaca
    24361 acaacctgtg cgaacacttt gcttattcgc gtcaggaact gttccatttg atccgcgttg
    24421 aaggcattaa aaccttcgaa gaactgctgg cgaaacacgg caaaggctac ggttgtgaag
    24481 tttgtaaacc aaccgtcggt tcgctgctgg cctcctgctg gaacgaatac attctgaagc
    24541 cggagcatac tccgctgcag gattctaacg acaacttcct cgctaacatc cagaaagacg
    24601 ggacctactc ggtgatcccg cgttctccgg gcggtgaaat caccccggaa gggctgatgg
    24661 cagtgggtcg tatcgcgcgt gaatttaatc tctacaccaa aatcaccggc tcccagcgcc
    24721 tggcgatgtt tggcgcacag aaagacgatc tgccggaaat ctggcgtcag ctgattgaag
    24781 ccggtttcga aaccggtcat gcctatgcga aagcactgcg tatggcgaaa acctgcgtgg
    24841 gtagcacctg gtgccgctac ggcgttggcg acagcgtcgg cctcggcgtg gaactggaaa
    24901 accgctacaa aggtatccgt acgccgcaca aaatgaagtt cggtgtctcc ggctgtaccc
    24961 gtgaatgttc agaagctcag ggtaaagacg tgggtattat cgccaccgaa aaaggctgga
    25021 acctgtacgt ttgcggtaac ggcggcatga aaccgcgtca tgcggatctg ctggcggcgg
    25081 atatcgatcg cgaaacgctg atcaaatatc tcgaccgctt catgatgttc tacatccgta
    25141 ctgccgacaa actgacgcgt accgcgccgt ggttagaaaa ccttgaaggc ggcatcgatt
    25201 acctgaaagc ggtgatcatt gacgacaaac tggggctgaa cgcacatctg gaagaagaga
    25261 tggcgcgcct gcgtgaagcg gtagtatgtg agtggactga gacggtcaat acaccgtccg
    25321 cgcagactcg cttcaaacac ttcatcaaca gcgacaagcg tgacccgaac gtgcagatgg
    25381 tgccagagcg cgaacagcac cgtccggcaa cgccgtatga acgtatcccg gtaactctgg
    25441 tggaggacaa cgcatgagcc agtggaaaga catctgcaaa atcgatgaca tcctgcctga
    25501 aaccggcgtc tgcgcgctgt taggtgacga gcaggtagca attttccgcc cgtatcacag
    25561 cgaccaggtg tttgcgatca gcaacatcga cccgttcttc gagtccagcg tgctgtcacg
    25621 cgggctgatt gcggaacacc agggcgagct gtgggtcgcc agcccgctga aaaagcagcg
    25681 ttttcgctta agcgacggct tgtgcatgga agacgaacag ttttccgtca agcattacga
    25741 cgcgcgagtg aaagacggcg tggtgcagct gcgcggttaa tattttaacg ggaggcgcaa
    25801 tgcctc
//
LOCUS       DADSYM010000064        25599 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2415
            SAMN08039579-rid11723173.denovo.064, whole genome shotgun sequence.
ACCESSION   DADSYM010000064 DADSYM010000000
VERSION     DADSYM010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039579
            Sequence Read Archive: SRR6362533
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 25599)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25599)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980162.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 03:39:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,484
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 4,347
            CDSs (with protein)               :: 4,347
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 43 of 85
            Pseudo Genes (incomplete)         :: 37 of 85
            Pseudo Genes (internal stop)      :: 19 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25599
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039579-rid11723173.denovo.064"
                     /strain="LGE2415"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8302 N 95.9628 W"
                     /collection_date="2015-08-24"
     gene            complement(231..1514)
                     /locus_tag="J1Y83_002222"
     CDS             complement(231..1514)
                     /locus_tag="J1Y83_002222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeaH/YhbH family protein"
                     /protein_id="HBA7630369.1"
                     /translation="MTWFIDRRLNGKNKSMVNRQRFLRRYKAQIKQSISEAINKRSVT
                     DVDSGESVSIPTEDISEPMFHQGRGGLRHRVHPGNDHFVQNDRIERPQGGGGGSGSGQ
                     GQASQDGEGQDEFVFQISKDEYLDLLFEDLALPNLKQNQQRQLTEYKTHRAGYTANGV
                     PANISVVRSLQNSLARRTAMTAGKRRELHALEENLAIISNSEPAQLLEEERLRKEIAE
                     LRAKIERVPFIDTFDLRYKNYEKRPDPSSQAVMFCLMDVSGSMDQSTKDMAKRFYILL
                     YLFLSRTYKNVEVVYIRHHTQAKEVDEHEFFYSQETGGTIVSSALKLMDEVVKERYNP
                     AQWNIYAAQASDGDNWADDSPLCHEILAKKILPVVRYYSYIEITRRAHQTLWREYEHL
                     QSTFDNFAMQHIRDQDDIYPVFRELFHKQNATAKD"
     gene            complement(1627..3561)
                     /gene="yeaG"
                     /locus_tag="J1Y83_002223"
     CDS             complement(1627..3561)
                     /gene="yeaG"
                     /locus_tag="J1Y83_002223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase YeaG"
                     /protein_id="HBA7630370.1"
                     /translation="MNIFDHYRQRYEAAKDEEFTLQEFLTTCRQDRSAYANAAERLLM
                     AIGEPVMVDTAQEPRLSRLFSNRVIARYPAFEEFYGMEDAIEQIVSYLKHAAQGLEEK
                     KQILYLLGPVGGGKSSLAERLKSLMQLVPIYVLSANGERSPVNDHPFCLFNPQEDAQI
                     LEKEYGIPRRYLGTIMSPWAAKRLHEFGGDITKFRVVKVWPSILQQIAIAKTEPGDEN
                     NQDISALVGKVDIRKLEHYAQNDPDAYGYSGALCRANQGIMEFVEMFKAPIKVLHPLL
                     TATQEGNYNGTEGISALPFNGIILAHSNESEWVTFRNNKNNEAFLDRVYIVKVPYCLR
                     ISEEIKIYEKLLNHSELTHAPCAPGTLETLSRFSILSRLKEPENSSIYSKMRVYDGES
                     LKDTDPKAKSYQEYRDYAGVDEGMNGLSTRFAFKILSRVFNFDHVEVAANPVHLFYVL
                     EQQIEREQFPQEQAERYLEFLKGYLIPKYAEFIGKEIQTAYLESYSEYGQNIFDRYVT
                     YADFWIQDQEYRDPDTGQLFDRESLNAELEKIEKPAGISNPKDFRNEIVNFVLRARAN
                     NSGRNPNWTSYEKLRTVIEKKMFSNTEELLPVISFNAKTSTDEQKKHDDFVDRMMEKG
                     YTRKQVRLLCEWYLRVRKSS"
     gene            3997..4743
                     /locus_tag="J1Y83_002224"
     CDS             3997..4743
                     /locus_tag="J1Y83_002224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MltA-interacting protein"
                     /protein_id="HBA7630371.1"
                     /translation="MTKLKLLALGVLIATSAGVAHAEGKFSLGAGVGVVEHPYKDYDT
                     DVYPVPVINYEGDNFWFRGLGGGYYLWNDATDKLSITAYWSPLYFKAKDSGDHQMRHL
                     DDRKSTMMAGLSYAHFTQYGYLRTTLAGDTLDNSNGIVWDMAWLYRYTNGGLTVTPGI
                     GVQWNSENQNEYYYGVSRKESARSGLRGYNPNDSWSPYLELSASYNFLGDWSVYGTAR
                     YTRLSDEVTDSPMVDKSWTGLISTGITYKF"
     gene            4833..5687
                     /gene="yeaE"
                     /locus_tag="J1Y83_002225"
     CDS             4833..5687
                     /gene="yeaE"
                     /locus_tag="J1Y83_002225"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal reductase YeaE"
                     /protein_id="HBA7630372.1"
                     /translation="MQQKMIQFSGDVSLPAIGQGTWYMGEDASQRKTEVAALRAGIEL
                     GLTLIDTAEMYADGGAEKVVGEALIGLRDNVFLVSKVYPWNAGGQKAINACEASLRRL
                     NTDYLDLYLLHWSGSFALEETVAAMEKLITQGKIRRWGVSNLDYADMQELWQLPGGNQ
                     CATNQVLYHLGSRGIEYDLLPWCQKQQMPVMAYSPLAQAGRLRNGLLKNAVVNEIAHA
                     HNISAAQVLLAWVISHQGVMAIPKAATVAHVQQNAAVLEVELSSAELVMLDKAYPAPK
                     GKTALDMV"
     gene            complement(5738..6622)
                     /locus_tag="J1Y83_002226"
     CDS             complement(5738..6622)
                     /locus_tag="J1Y83_002226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-hexose-6-phosphate mutarotase"
                     /protein_id="HBA7630373.1"
                     /translation="MIKKIFALPVIEQISPVLSRRKLDELDLIVVDHPQVKASFALQG
                     AHLLSWKPAGEEEVLWLSNNTPFKNGVAIRGGIPVCWPWFGPAAQQGLPAHGFARNLP
                     WTLKSHREDADGVALTFELTQSEETKKFWPHDFTLLAHFHVGKTCEIDLEAHGEFETT
                     SALHTYFNVGDIAKVSVSGLGDRFIDKVNDAKEDVLTDGIQTFPDRTDRVYLNPQDCS
                     VINDEALNRIIAVGHQHHLNVVGWNPGPALSVSMGDMPDDGYKTFVCVETAYASETQK
                     VTKEKPAHLAQSIRVAKR"
     gene            complement(6706..7701)
                     /gene="gapA"
                     /locus_tag="J1Y83_002227"
     CDS             complement(6706..7701)
                     /gene="gapA"
                     /locus_tag="J1Y83_002227"
                     /EC_number="1.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HBA7630374.1"
                     /translation="MTIKVGINGFGRIGRIVFRAAQKRSDIEIVAINDLLDADYMAYM
                     LKYDSTHGRFDGTVEVKDGHLIVNGKKIRVTAERDPANLKWDEVGVDVVAEATGLFLT
                     DETARKHITAGAKKVVMTGPSKDNTPMFVKGANFDKYAGQDIVSNASCTTNCLAPLAK
                     VINDNFGIIEGLMTTVHATTATQKTVDGPSHKDWRGGRGASQNIIPSSTGAAKAVGKV
                     LPELNGKLTGMAFRVPTPNVSVVDLTVRLEKAATYEQIKAAVKAAAEGEMKGVLGYTE
                     DDVVSTDFNGEVCTSVFDAKAGIALNDNFVKLVSWYDNETGYSNKVLDLIAHISK"
     gene            8043..8456
                     /gene="msrB"
                     /locus_tag="J1Y83_002228"
     CDS             8043..8456
                     /gene="msrB"
                     /locus_tag="J1Y83_002228"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HBA7630375.1"
                     /translation="MANKPSAEELKKNLSEMQFYVTQNHGTEPPFTGRLLHNKRDGVY
                     HCLICDAPLFHSQTKYDSGCGWPSFYEPVSEESIRYIKDLSHGMQRIEIRCGNCDAHL
                     GHVFPDGPQPTGERYCVNSASLRFTDGENGEEING"
     gene            8498..8770
                     /locus_tag="J1Y83_002229"
     CDS             8498..8770
                     /locus_tag="J1Y83_002229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeaC family protein"
                     /protein_id="HBA7630376.1"
                     /translation="MNLDDIINSMTPEVYQRLSTAVELGKWPDGVTLTEEQKENCLQL
                     VMLWQARHNTEAQHMTIDTNGQMVMKSKQQLKEDFGISAKPIAMFK"
     gene            9140..10216
                     /locus_tag="J1Y83_002230"
     CDS             9140..10216
                     /locus_tag="J1Y83_002230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HBA7630377.1"
                     /translation="MKALARFGKAFGGYKMIDVPQPICGPEDVVIEIKAAAICGADMK
                     HYNVDSGSDEFNSIRGHEFAGCIAQVGEKVKDWKVGQRVVSDNSGHVCGVCPACEQGD
                     FLCCTEKVNLGLDNNTWGGGFSKYCLVPGEILKIHRHALWEIPDSVDYEDAAVLDPIC
                     NAYKSIAQQSKFLPGQDVVVIGTGPLGLFSVQMARIMGAVNIVVVGLQEDVAVRFPVA
                     KELGATAVVNGSTEDVVARSQQICGKDNLGLVIECSGANIALKQAIEMLRPNGEVVRV
                     GMGFKPLDFSINDITAWNKSIIGHMAYDSTSWRNAIRLLASGAIKVKPMITHRIGLSQ
                     WREGFDAMVDKTAIKVIMTYDFDE"
     gene            10243..11622
                     /locus_tag="J1Y83_002231"
     CDS             10243..11622
                     /locus_tag="J1Y83_002231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBA7630378.1"
                     /translation="MEQITKPHCGARLDRLPDCRWHSSMFAIVAFGLLVCWSNAVGGL
                     ILAQLKALGWTDNSTTATFSAITTAGMFLGALVGGIIGDKTGRRNAFILYEAIHIASM
                     VVGAFSPNMDFLIACRFVMGVGLGALLVTLFAGFTEYMPGRNRGTWSSRVSFIGNWSY
                     PLCSLIAMGLTPLISAEWNWRVQLLIPAILSLIATALAWRYFPESPRWLESRGRYQEA
                     EKVMRSIEEGVIRQTGKPLPPVVIADDGKAPQAVPYSALLTGVLLKRVILGSCVLIAM
                     NVVQYTLINWLPTIFMTQGINLKDSIVLNTMSMFGAPFGIFIAMLVMDKIPRKTMGVG
                     LLILIAVLGYIYSLQTSMLLITLIGFFLITFVYMYVCYASAVYVPEIWPTEAKLRGSG
                     LANAVGRISGIAAPYAVAVLLSSYGVTGVFILLGAVSIIVAIAIATIGIETKGVSVES
                     LSIDAVANK"
     gene            11639..12682
                     /locus_tag="J1Y83_002232"
     CDS             11639..12682
                     /locus_tag="J1Y83_002232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent alcohol dehydrogenase"
                     /protein_id="HBA7630379.1"
                     /translation="MKNSKAILQVPGTMKIISAEIPVPKEDEVLIKVEYVGICGSDVH
                     GFESGPFIPPKDPNQEIGLGHECAGTVVAVGSRVRKFKPGDRVNIEPGVPCGHCRYCL
                     EGKYNICPDVDFMATQPNYRGALTHYLCHPESFTYKLPDNMDTMEGALVEPAAVGMHA
                     AMLADVKPGKKIIILGAGCIGLMTLQACKCLGATEIAVVDVLEKRLTMAEQLGATVVI
                     NGGKEDTIARCQQFTEDMGADIVFETAGSAVTVKQAPYLVMRGGKIMIVGTVPGDSAI
                     NFLKINREVTIQTVFRYANRYPVTIEAISSGRFDVKSMVTHIYDYRDVQQAFEESVNN
                     KRDIIKGVIKISD"
     gene            12703..13539
                     /locus_tag="J1Y83_002233"
     CDS             12703..13539
                     /locus_tag="J1Y83_002233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HBA7630380.1"
                     /translation="MLADIRYWENDATNKHYAIAHFNVWNAEMLMGVIDAAEEAKSPV
                     IISFGTGFVGNTSFEDFSHMMVSMAQKATVPVITHWDHGRSMEIIHNAWTHGMNSLMR
                     DASAFDFEENIRLTKEAVDFFHPLGIPVEAELGHVGNETVYEEALAGYHYTDPDQAAE
                     FVERTGCDSLAVAIGNQHGVYTSEPQLNFEVVKRVRDAVSVPLVLHGASGISDADIKT
                     AISLGIAKINIHTELCQAAMVAVKENQDQPFLHLEREVRKAVKERALDKIKLFGSDGK
                     AE"
     gene            13544..14491
                     /locus_tag="J1Y83_002234"
     CDS             13544..14491
                     /locus_tag="J1Y83_002234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416286.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HBA7630381.1"
                     /translation="MDNLDVICIGAAIVDIPLQPVSKNIFDVDSYPLERIAMTTGGDA
                     INEATIISRLGHRTALMSRIGKDAAGQFILDHCRKENIDIQSLKQDVSIDTSINVGLV
                     TEDGERTFVTNRNGSLWKLNIDDVDFARFSQAKLLSLASIFNSPLLDGRALTEIFTQA
                     KTRQMIICADMIKPRLNETLNDICEALSYVDYLFPNFAEAKLLTGKETLDEIADCFLA
                     CGVKTVVIKTGKDGCFIKRGDMTMKVPAVAGITAIDTIGAGDNFASGFIAALLEGKNL
                     RECARFANATAAISVLSIGATTGVKNRKLVEQLLEEYEG"
     gene            14501..15481
                     /gene="ydjG"
                     /locus_tag="J1Y83_002235"
     CDS             14501..15481
                     /gene="ydjG"
                     /locus_tag="J1Y83_002235"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent methylglyoxal reductase"
                     /protein_id="HBA7630382.1"
                     /translation="MKKIPLGTTDITLSRMGLGTWAIGGGPAWNGDLDRQICIDTILE
                     AHRCGINLIDTAPGYNFGNSEVIVGQALKKLPREQVVVETKCGIVWERKGSLFNKVGE
                     RQLYKNLSPESIREEVEASLQRLGIDYIDIYMTHWQSVPPFFTPIAETVAVLNALKAE
                     GKIRAIGAANVDADHIREYLQHGELDIIQAKYSILDRAMENELLPLCRDNGIVVQVYS
                     PLEQGLLTGTITRDYVPGGARANKVWFQRENMLKVIDMLEQWQPLCARYQCTIPTLAL
                     AWILKQSDLISILSGATAPEQVRENVAALNINLSDADATLMREMAEALER"
     gene            15618..16376
                     /locus_tag="J1Y83_002236"
     CDS             15618..16376
                     /locus_tag="J1Y83_002236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HBA7630383.1"
                     /translation="MAAKDRIQAIKQMVANDKKVTVSNLSGIFQVTEETIRRDLEKLE
                     DEGFLTRTYGGAVLNTAMLTENIHFYKRASSFYEEKQLIARKALPFIDNKTTMAADSS
                     STVMELLKLLQDRSDLTLLTNSAEAIHVLAQSEIKVVSTGGELNKNTLSLQGRITKEI
                     ISRYHVDIMVMSCKGLDINSGALDSNEAEAEIKKTMIRQATEVALLVDHSKFDRKAFV
                     QLADFSHINYIITDKSPGAEWIAFCKDNNIQLVW"
     gene            16494..17852
                     /locus_tag="J1Y83_002237"
     CDS             16494..17852
                     /locus_tag="J1Y83_002237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBA7630384.1"
                     /translation="MEQYDQIGARLDRLPLARFHYRIFGIISFSLLLTGFLSYSGNVV
                     LAKLVSNGWSNNFLNAAFTSALMFGYFIGSLTGGFIGDYFGRRRAFRINLLIVGIAAT
                     GAAFVPDMYWLIFFRFLMGTGMGALIMVGYASFTEFIPATVRGKWSARLSFVGNWSPM
                     LSAAIGVVVIAFFSWRIMFLLGGIGILLAWFLSGKYFIESPRWLAGKGQIAGAESQLR
                     EVEQQIEREKSIRLPPLTLNQSNSKVKVIKGTFWLLFKGEMLRRTLVAITVLIAMNIS
                     LYTITVWIPTIFVNSGIDVDKSILMTAVIMIGAPVGIFIAALIIDHFPRRLFGSALLI
                     IIAVLGYIYSIQTTEWAILIYGLVMIFFLYMYVCFASAVYIPELWPTHLRLRGSGFVN
                     AVGRIVAVFTPYGVAALLTHYGSITVFMVLGVMLVLCALVLSIFGIETRKVSLEEISE
                     VN"
     gene            complement(17945..18586)
                     /gene="pncA"
                     /locus_tag="J1Y83_002238"
     CDS             complement(17945..18586)
                     /gene="pncA"
                     /locus_tag="J1Y83_002238"
                     /EC_number="3.5.1.-"
                     /EC_number="3.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001587135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nicotinamidase/pyrazinamidase"
                     /protein_id="HBA7630385.1"
                     /translation="MPPRALLLVDLQNDFCAGGALAVPEGDSTVDVANRLIDWCQSRG
                     EAVIASQDWHPANHGSFASQHGVEPYTPGQLDGLPQTFWPDHCVQNSEGAQLHPLLKQ
                     KAIAAVFHKGENPLVDSYSAFFDNGRRQKTALDDWLRAHVINELIVMGLATDYCVKFT
                     VLDALQLGYKVNVITDGCRGVNIQPQDSAHAFMEMSAAGATLYTLADWEETQE"
     gene            complement(18597..19613)
                     /gene="ansA"
                     /locus_tag="J1Y83_002239"
     CDS             complement(18597..19613)
                     /gene="ansA"
                     /locus_tag="J1Y83_002239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HBA7630386.1"
                     /translation="MQKKSIYVAYTGGTIGMQRSEQGYIPVSGHLQRQLALMPEFHRP
                     EMPDFTIHEYTPLMDSSDMTPEDWQHIAEDIKAHYDDYDGFVILHGTDTMAYTASALS
                     FMLENLGKPVIVTGSQIPLAELRSDGQINLLNALYVAANYPINEVTLFFNNRLYRGNR
                     TTKAHADGFDAFASPNLPPLLEAGIHIRRLNTPPAPHGEGELIVHPITPQPIGVVTIY
                     PGISADVVRNFLRQPVKALILCSYGVGNAPQNKAFLQELQEASDRGILVVNLTQCMSG
                     KVNMGGYATGNALAHAGVIGGADMTVEATLTKLHYLLSQELDTETIRKAMSQNLRGEL
                     TPDD"
     gene            complement(19780..21636)
                     /gene="sppA"
                     /locus_tag="J1Y83_002240"
     CDS             complement(19780..21636)
                     /gene="sppA"
                     /locus_tag="J1Y83_002240"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707347.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HBA7630387.1"
                     /translation="MRTLWRFIAGFFKWTWRLLNFVREMVLNLFFIFLVLVGVGIWMQ
                     VSGGDSKETASRGALLLDISGVIVDKPDSSQRFSKLSRQLLGASSDRLQENSLFDIVN
                     TIRQAKDDRNITGIVMDLKNFAGGDQPSMQYIGKALKEFRDSGKPVYAVGENYSQGQY
                     YLASFANKIWLSPQGVVDLHGFATNGLYYKSLLDKLKVSTHVFRVGTYKSAVEPFIRD
                     DMSPAAREADSRWIGELWQNYLNTVAANRQLPAQQVFPGAQGLLEGLTKTGGDTAKYA
                     LENKLVDALASSAEIEKTLTKEFGWSKTDKNYRAISYYDYALKTPADTGDSIGVVFAN
                     GAIMDGEETQGNVGGDTTAAQIRDARLDPKVKAIVLRVNSPGGSVTASEVIRAELAAA
                     RAAGKPVVVSMGGMAASGGYWISTPANYIVANPSTLTGSIGIFGVITTVENSLDSIGV
                     HTDGVSTSPLADVSITKALPPEAQQMMQLSIENGYKRFITLVADARHSTPEQIDKIAQ
                     GHVWTGQDAKANGLVDSLGDFDDAVAKAAELAKVKQWHLEYYVDEPTFFDKVMDNMSG
                     SIRAMLPDAFQAMLPAPLASVASTVKSESDKLAAFNDPQNRYAFCLTCANVR"
     gene            21797..22348
                     /locus_tag="J1Y83_002241"
     CDS             21797..22348
                     /locus_tag="J1Y83_002241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H nitroreductase"
                     /protein_id="HBA7630388.1"
                     /translation="MDALELLINRRSASRLAEPAPTGEQLQNILRAGMRAPDHKSMQP
                     WHFFVIEGEGRERFSAVLEQGAIAAGSDNKAIDKARNAPFRAPLIITVVAKCEENHKV
                     PRWEQEMSAGCAVMAMQMAAVAQGFGGIWRSGALTESPVVREAFGCREQDKIVGFLYL
                     GTPQLKASTSINVPDPTPFVTYF"
     gene            22465..23508
                     /gene="selD"
                     /locus_tag="J1Y83_002242"
     CDS             22465..23508
                     /gene="selD"
                     /locus_tag="J1Y83_002242"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HBA7630389.1"
                     /translation="MSENSIRLTQYSHGAGCGCKISPKVLETILHSEQAKFVDPNLLV
                     GNETRDDAAVYDLGNGTSVISTTDFFMPIVDNPFDFGRIAATNAISDIFAMGGKPIMA
                     IAILGWPINKLSPEIAREVTEGGRYACRQAGIALAGGHSIDAPEPIFGLAVTGIVPTE
                     RVKKNSTAQAGCKLFLTKPLGIGVLTTAEKKSLLKPEHQGLATEVMCRMNIAGASFAN
                     IEGVKAMTDVTGFGLLGHLSEMCQGAGVQARVDYDAIPKLPGVEEYIKLGAVPGGTER
                     NFASYGHLMGEMPREVRDLLCDPQTSGGLLLAVMPEAENEVKATAAEFGIELTAIGEL
                     VPARGGRAMVEIR"
     gene            23513..25459
                     /locus_tag="J1Y83_002243"
     CDS             23513..25459
                     /locus_tag="J1Y83_002243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001515429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HBA7630390.1"
                     /translation="MRLFIAEKPSLARAIADVLPKPHRKGDGFIECGNGQVVTWCIGH
                     LLEQAQPDAYDSRYARWNLADLPIVPEKWQLQPRPSVTKQLNVIKRFLHEASEIVHAG
                     DPDREGQLLVDEVLDYLQLAPEKRQQVQRCLINDLNPQAVERAIDRLRSNSEFVPLCV
                     SALARARADWLYGINMTRAYTILGRNAGYQGVLSVGRVQTPVLGLVVRRDEEIENFVA
                     KDFFEVKAHIVTPADERFTAIWQPSEACEPYQDEEGRLLHRPLAEHVVNRISGQPAIV
                     TSYNDKRESESAPLPFSLSALQIEAAKRFGLSAQNVLDICQKLYETHKLITYPRSDCR
                     YLPEEHFAGRHAVMNAISVHAPDLLPQPVVDPDIRNRCWDDKKVDAHHAIIPTARSSA
                     INLTENEAKVYNLIARQYLMQFCPDAVFRKCVIELDIAKGKFVAKARFLAEAGWRTLL
                     GSKERDEENDGTPLPVVAKGDELLCEKGEVVERQTQPPRHFTDATLLSAMTGIARFVQ
                     DKDLKKILRATDGLGTEATRAGIIELLFKRGFLTKKGRYIHSTDAGKALFHSLPEMAT
                     RPDMTAHWESVLTQISEKQCRYQDFMQPLVGTLYQLIDQARSTPVRQFRGIVASGGGA
                     VKKAPNKRVKKAAKPKTDNTDSAN"
BASE COUNT         6470 a         5946 c         6570 g         6613 t
ORIGIN      
        1 atagttttga ccaggatcaa ttttcgttga cgataaaaaa taattaaaga ataaccccag
       61 ggaaatacgt gttgaccgaa tcatttttcc gaatgattgt tttctggaac taaattgtgt
      121 aaaggttatg ctgtcatatt taaagcgatt gtaagctaat gtatgtaata aatgagataa
      181 tttacagtga gctaaaaatc agccaggcga taatgacccg gctgacagtt ttagtcttta
      241 gctgttgcat tttgtttatg aaacagttca cggaacaccg gataaatatc atcctggtcg
      301 cggatatgct gcatcgcaaa gttgtcgaaa gtagattgca gatgctcata ttctcgccac
      361 agtgtctgat gcgcgcgacg tgtaatttcg atatagctgt aataacgaac aacgggtaat
      421 atttttttcg ccagaatttc atggcaaagc ggagagtcat cggcccagtt atcgccgtct
      481 gatgcttggg ctgcgtaaat attccactgt gccgggttat aacgctcttt cactacctca
      541 tccatcagtt tcagggcgct ggaaacaatg gtgccgccag tttcctgcga gtagaaaaac
      601 tcatgttcat cgacttcttt cgcctgggta tgatggcgga tgtatacgac ttccacgttc
      661 ttatacgttc tgctgaggaa cagatacagc agaatataaa aacgtttagc catatcctta
      721 gtggactgat ccattgaacc ggaaacgtcc atcaggcaaa acatcactgc ctggctggag
      781 ggatcgggcc gtttttcgta gttcttgtaa cgtaaatcga aggtgtcaat gaaagggacg
      841 cgttcaattt tggcacgtaa ttctgcaatt tctttgcgca gacgttcctc ttccagcagt
      901 tgcgcaggtt cactgttgct tatgatggcc aaattctctt ccagcgcatg aagttctcgc
      961 cgcttgcccg ccgtcatggc tgtgcgccgc gccagtgagt tctgcaatga acgtacaacg
     1021 ctgatattgg ccggaacgcc gttagcggta taacccgccc gatgcgtttt atattcggtc
     1081 agctggcgtt gttggttttg tttcagattc ggtaaggcca aatcttcaaa gagcagatca
     1141 agatactcat ctttcgaaat ctgaaaaaca aattcatcct gaccttcacc atcctggctg
     1201 gcctggccct gaccactgcc ggaacctccg ccaccaccct ggggacgttc aattcggtcg
     1261 ttctggacga aatggtcatt gcccggatgc acacggtggc gcaggcctcc acgcccctga
     1321 tgaaacatcg gttcgctaat atcttccgtg ggaatggata cggactcgcc gctgtcgacg
     1381 tcagtcaccg aacgcttatt tatggcctcg gagatcgact gtttaatttg cgctttataa
     1441 cggcgtaaga aacgctggcg attcaccatg cttttatttt tgccgttcag acgccggtca
     1501 ataaaccagg tcatatgccc cccgtactac atttgccaac ggtatgctac gcctgtgttg
     1561 ccgtttctct ggcccacggt tcgctgtcgt tgtaggcccg gtagcatgtg taccgggcca
     1621 gggttgttaa gacgatttac gtacgcgcaa ataccattcg cacagtaaac gcacctgttt
     1681 acgggtgtag cctttttcca tcatacggtc gacaaagtcg tcgtgtttct tctgctcgtc
     1741 ggttgacgtt ttggcgttaa acgaaataac cggcaacagc tcttcggtat tggagaacat
     1801 tttcttctcg ataaccgtgc gcagtttttc atagctggtc cagttcggat tacgtccgct
     1861 gttattcgct ctggctcgca gtacgaagtt aacaatctcg ttgcggaaat cttttggatt
     1921 actgatcccc gccggtttct cgattttctc cagctcggcg ttaagagact cgcggtcaaa
     1981 caactgcccg gtatccggat cgcgatactc ctgatcctga atccagaaat ccgcgtaggt
     2041 aacataacgg tcaaaaatgt tctgcccata ttctgaatag gattcaaggt aggccgtctg
     2101 gatctctttg ccgataaact cggcatattt cgggatcaga taacctttca ggaactccag
     2161 atagcgttct gcctgctctt gcgggaactg ctcgcgctcg atctgctgtt ccaggacgta
     2221 gaacagatgg accgggtttg ccgccacttc tacatgatcg aagttgaaca cgcgggagag
     2281 gatcttaaac gcaaaacgcg tcgacagacc gttcatccct tcatcgacac cggcgtagtc
     2341 acgatattcc tgatacgact tggctttggg atcagtgtct ttcaaacttt caccatcata
     2401 aacccgcatc tttgaataaa tgctggagtt ttctggctct ttcagacgcg aaagaataga
     2461 aaaacgtgac agcgtttcca gcgtgccagg ggcgcagggg gcgtgagtca attcactgtg
     2521 attaagcaat ttctcgtaga ttttgatctc ttcggaaatg cgcaagcaat acggcacctt
     2581 cacgatgtaa acacggtcga ggaaggcttc gttgtttttg ttattacgga aagtgaccca
     2641 ttcggattcg ttcgagtggg cgagaataat cccgttgaac ggcagggcgg agataccttc
     2701 cgtcccgttg tagttacctt cctgggtggc ggttaacaag ggatgcagca cttttatcgg
     2761 tgctttaaac atctcgacaa attccatgat cccctgattg gcgcggcaca gcgcaccgga
     2821 atagccgtag gcgtccgggt cattctgcgc gtagtgttcg agtttacgaa tatcgacttt
     2881 ccctaccagg gcggagatgt cctggttgtt ctcatcgccg ggttctgttt tggcgatagc
     2941 aatttgttgc agaattgacg gccagacctt cactacccgg aacttagtga tatcgccacc
     3001 aaattcatgc aggcgttttg ccgcccacgg cgacatgatg gtgccgagat aacggcgagg
     3061 aatgccatac tctttttcca gaatctgcgc atcttcctgc ggattgaaaa gacagaacgg
     3121 atgatcgttg accgggctac gctcaccgtt ggcgctcaat acataaatcg gtacgagctg
     3181 cattaatgat ttcagtcgct cagcaagcga tgatttaccc ccacccacag gccccagcag
     3241 atacaggatt tgtttcttct cttccagccc ctgagccgcg tgtttcagat aagagacaat
     3301 ctgttcaatc gcgtcttcca tgccgtaaaa ctcttcaaac gccggataac gtgcaatgac
     3361 ccggttagaa aagagtcgag aaagtctggg ttcctgggct gtatcgacca tgacaggctc
     3421 accaatagcc atcaatagcc gctcagccgc gttggcataa gcactgcgat cttgccgaca
     3481 agtggtaaga aactcctgca gtgtgaactc ttcgtccttg gcagcttcat atcgctggcg
     3541 atagtgatcg aatatattca tggtatgccg tcctttcgtt ttttagcaca ggttaagagc
     3601 cgttcatatg aatagttgag gctcccggaa gaggtaaact gaacgacgtc tcttgctaaa
     3661 acacagcaac tctcatgcca ttttattgac gatgagaaca acgtgcccgg tgatacacct
     3721 tcaaaattaa gcgtagatgg caaatggaaa acttgcctgc acggctttct taatttcaat
     3781 ggcatatcaa caactcatcc ataaattttg cataattaat gtaaataata gggtcgccgg
     3841 taacgctaaa ttcatttggc tgtaagcgtg gtgtcatctg cgtcaggaaa attaaacagt
     3901 tactataaaa aatgaaaacg taaaaaggtt gggtttcgat gtattgacgg gtaaactttg
     3961 tcgcccgcta aacatttgtt taaggaatga ttaattgtga ccaaactcaa acttctggca
     4021 cttggagtgc ttatcgcaac gtctgcaggc gtagcgcacg ctgaaggtaa attttccctg
     4081 ggcgcaggcg taggtgtcgt tgaacaccca tataaagatt acgataccga tgtttaccca
     4141 gtaccggtaa tcaactatga aggcgataac ttctggttcc gtggcttagg tggtggttac
     4201 tacctgtgga atgacgcaac ggacaaactt tcaattaccg cttactggtc gccgctttac
     4261 ttcaaagcta aagacagtgg cgatcaccaa atgcgtcacc tggatgaccg taagagcacc
     4321 atgatggctg gtctgtctta tgctcacttt acccagtacg gttacctgcg taccactctg
     4381 gctggcgata ccctggataa cagcaacggc atcgtctggg atatggcctg gttgtatcgt
     4441 tacaccaacg gtggcctgac cgtgactccg ggtattggtg tgcagtggaa cagcgaaaac
     4501 cagaacgaat actattatgg cgtatcgcgc aaagagtccg ctcgcagcgg tctgcgtggc
     4561 tataacccga acgacagctg gagcccttac ctggagctga gcgccagcta caacttcctc
     4621 ggtgactgga gtgtttacgg taccgcgcgc tacacccgtc tgtctgatga agttactgac
     4681 agcccaatgg tggataaatc ctggactggc ctgatttcta ccgggatcac ctacaaattc
     4741 tgataatgca tttttccagg gcattttgtg cataaaaaca gggcgtaatg ccctgttttg
     4801 cattacggtg gtgcaatacc tgggaggaag caatgcaaca aaaaatgatt caatttagtg
     4861 gcgatgtctc actgccagcc atagggcagg gaacatggta tatgggcgaa gatgccagtc
     4921 agcgtaaaac agaagttgct gcactacgcg cgggcattga actcggttta accctcattg
     4981 ataccgccga aatgtatgcc gatggcggtg ccgaaaaagt ggttggggaa gcattaatcg
     5041 gtctgcgtga taacgtcttt ctcgtctcta aagtctatcc gtggaatgct ggcgggcaaa
     5101 aagcgataaa tgcatgcgaa gccagtttac gccgtctcaa tactgattat ctcgatctct
     5161 acttattaca ctggtctggc agtttcgctc tcgaagagac tgtcgcagcg atggaaaaat
     5221 tgatcaccca gggaaaaatc cgccgctggg gcgtttctaa ccttgattat gccgatatgc
     5281 aggaactctg gcagctgccg gggggaaatc agtgtgccac taatcaggtg ctttaccatc
     5341 tcggttcacg agggattgag tacgatctac ttccctggtg ccagaaacag cagatgccgg
     5401 tgatggctta cagtccatta gcccaggccg ggcggttgcg caatggactg ttaaaaaatg
     5461 cggtagtcaa cgaaattgca catgctcaca atatcagtgc ggcacaagta ttgttggcgt
     5521 gggtaatcag ccatcagggt gtgatggcga ttccaaaagc ggcaactgtc gcacatgtcc
     5581 aacaaaatgc ggctgtgctt gaggttgaac tttcgtcagc ggaattagtt atgctggata
     5641 aagcgtatcc ggcaccaaaa ggaaaaactg cgctggatat ggtgtgatgt tgatacgtgc
     5701 cggatgcatc gcacccggca cacaatatta acgcaaatta acgtttcgca acgcgaatgg
     5761 attgcgccag atgtgcaggt ttctctttgg tgactttttg cgtttctgaa gcgtaagccg
     5821 tttctacaca aacaaaggtc ttgtagccat catccggcat atcgcccatg ctaactgaaa
     5881 gcgccggtcc tgggttccag cctacaacgt tcagatgatg ctggtggcct acggcgataa
     5941 tacgattcag cgcttcgtca ttaattacgc tgcaatcttg cggattcaga tacacgcgat
     6001 cggtacggtc agggaaggtc tgaataccgt cggtcagtac atcttctttc gcgtcattta
     6061 ctttatcaat gaagcgatcg cctagtccac tgacgcttac cttagcgata tcacccacgt
     6121 taaagtaggt atgcagagca gaggtagttt caaattcgcc gtgtgcttca agatcgattt
     6181 cacaggtttt acccacgtgg aaatgcgcta acagcgtaaa gtcgtgcggc cagaattttt
     6241 tcgtctcttc gctttgtgtc aattcaaaag tcagcgctac gccatcagca tcttcacgat
     6301 gtgatttcag cgtccacggc aggttgcggg caaaaccgtg cgcaggcaga ccttgttgtg
     6361 cagccggacc aaaccacggc cagcaaaccg gtatgccacc gcgaatggcg acgccatttt
     6421 tgaacggtgt gttgttactc aaccacagaa cttcttcttc acccgcaggt ttccacgaga
     6481 gaaggtgtgc gccctgtaat gcaaaagagg cttttacctg gggatgatcg accacaatga
     6541 ggtccagttc atccagttta cgacgggaga ggacagggga gatttgttcg atgaccggaa
     6601 gggcaaaaat tttcttaatc atgacgcagt cctttaactt cattttatca ggtaaaaaaa
     6661 agagcgaccg aagtcgctct ttttagatca cagagtcatc tcaacttatt tggagatgtg
     6721 agcgatcagg tccagaactt tgttggagta accggtttcg ttgtcgtacc aggataccag
     6781 tttcacgaag ttgtcgttca gagcgatacc cgctttagca tcgaacacgg aagtgcaaac
     6841 ttcgccgttg aaatcggtag atactacgtc atcttcggtg tagcccagaa cgcctttcat
     6901 ttcgccttca gcagcagctt taacggcagc tttgatctgc tcgtaagttg cagctttttc
     6961 cagacgaacg gtcaggtcaa ctacggatac gttcggggtc ggaacgcgga acgccatacc
     7021 agtcagtttg ccattcagtt ctggcagtac tttacctaca gctttagcag caccggtaga
     7081 agacgggatg atgttctggg aagcgccgcg gccgccgcgc cagtctttgt gagacgggcc
     7141 atcaacggtt ttctgagtag cggtagtagc gtgaacggtg gtcatcagac cttcgatgat
     7201 gccgaagtta tcgttgataa ctttagccag cggagccagg cagttggtgg tgcaggaagc
     7261 gttggaaacg atgtcctggc cagcatattt gtcgaagtta gcgcctttaa cgaacatcgg
     7321 agtgttgtct ttagacggac cagtcataac cactttcttc gcaccagcgg tgatgtgttt
     7381 acgagcagtt tcgtcagtca ggaacagacc agttgcttca gcgacaacgt caacaccaac
     7441 ttcgtcccat ttcaggttag ccggatcacg ttcagcggta acacggattt ttttaccgtt
     7501 aacgatcaga tgaccgtctt tcacttcaac ggtaccgtcg aaacggccgt gagtggagtc
     7561 atatttcagc atgtatgcca tgtaatcagc gtctaacagg tcgttgattg caacgatctc
     7621 gatgtcagaa cgtttctgag cagcacggaa aacaatgcga ccgatacggc caaaaccgtt
     7681 gatacctact ttgatagtca tatattccac cagctatttg ttagtgaata aaaggttgcc
     7741 tgtaaaatta caaaaacctt acgcagcgtc aagcggaatc gtgtcaatca ttgcgacaaa
     7801 tcaatcctgt gcctaagcat tacgcgactg actcgcctca ctcttccttt gggcttgaga
     7861 ccacatgggg tcggcgcccc gaattttaaa gggcaattac gataaaaatg tgattttcat
     7921 cacgatttag gtgcagccat ttcgtcgaga tcaagtttag gacaaaattt tgccagttgg
     7981 tgttaatgtt ttgttagaat cagtcacgta atgtgagcac gattaaagtg agatgtgagc
     8041 aaatggctaa taaaccttcg gcagaagaac tgaaaaaaaa tttgtccgag atgcagttct
     8101 acgtgacgca gaatcatggg acagaaccgc catttacggg tcgtttactg cataacaagc
     8161 gtgacggcgt atatcactgt ttgatctgcg atgccccgct gtttcattcc caaaccaagt
     8221 atgattccgg ctgtggctgg cccagcttct acgaaccggt gagtgaagaa tctattcgtt
     8281 atatcaaaga cttgtcgcat ggaatgcagc gcatagaaat tcgttgcggt aactgcgatg
     8341 cccatctggg ccatgtcttt cctgacgggc cgcagccaac gggcgaacgt tattgtgtta
     8401 actctgcttc tttacgcttc actgatggcg aaaacggcga agaaatcaac ggttgaaaaa
     8461 acgattcagc aacttattcc acaggagcgg aaaaattatg aatcttgatg acattatcaa
     8521 cagcatgacg cctgaggtat accagcgttt gtcgaccgcc gttgaactgg ggaaatggcc
     8581 tgatggcgtt acgttgaccg aggaacaaaa agagaactgc ctgcaactgg tgatgctgtg
     8641 gcaagcccgc cataataccg aagcacagca tatgacaatt gacacaaatg gtcagatggt
     8701 catgaagagc aaacagcagt taaaagaaga ttttggtatc tcagcaaagc ctattgccat
     8761 gtttaagtaa gtggtttatc ccctttttgc cgcggttata aaggggatag ccaacaatga
     8821 ccccacgact catctcaatt atctcgcccc gacctggcta agtcccagtc cgatctcttg
     8881 cagtcgcgtt cacacctgaa tgacttctcc tgaacttaag tctcaggcct atttggccgg
     8941 gaatccctct cgaatttgct ctttttgcgt cccgccttcc atcaacctgt aacagcccca
     9001 gccattttct gcgtaattgt gatctaagtt gtaaaacgca gatcattatc tgtgttttgc
     9061 agatttaatg ttgacctcaa aaatcatgtg ggtaagtatg atgaccatct aagcaaaaca
     9121 aaacacagag gatgacgcaa tgaaagcact ggctcggttt ggcaaggcct ttggcggcta
     9181 caagatgatt gatgtcccac aacccatttg tggcccggaa gatgtcgtga ttgaaattaa
     9241 agccgcggca atctgcggcg cagacatgaa gcactacaat gtcgatagcg gttcggatga
     9301 gtttaactct atccgcggcc atgagttcgc aggttgtatt gcgcaggttg gtgaaaaagt
     9361 caaagactgg aaagtggggc aacgcgtcgt gtcggataac agcggtcacg tttgcggcgt
     9421 ttgcccggcc tgtgaacaag gtgattttct gtgttgtaca gaaaaagtaa accttggtct
     9481 ggataacaat acctggggcg gtggtttttc caaatattgt cttgttcctg gtgaaattct
     9541 caaaattcat cgtcatgcgt tgtgggaaat ccctgatagt gttgattatg aggacgcagc
     9601 cgtacttgac cctatctgta atgcctataa atccatcgcg cagcaatcga aattcctccc
     9661 tggtcaggat gtagtcgtca tcggtactgg cccactcggg ctgttctccg tacaaatggc
     9721 gcggattatg ggggcggtaa atatcgtcgt cgttggtcta caagaagatg tggcggtccg
     9781 cttcccggtt gcaaaagaac tgggtgcgac ggcagtagta aatggttcta ccgaagatgt
     9841 ggtggcgcgc agccagcaaa tttgtggcaa agacaatctg ggactggtga ttgaatgctc
     9901 cggtgccaat atcgcactga aacaagccat cgaaatgctc cgtccgaacg gggaagtggt
     9961 acgcgttgga atgggcttca aacctcttga tttctcgatt aatgacatta ccgcctggaa
    10021 caaaagcatc attgggcata tggcctatga ctccacctca tggcgtaacg ctatcaggct
    10081 attagccagc ggcgcgatca aagtcaaacc gatgatcacg catcgtatcg gcctgtcgca
    10141 atggcgcgaa gggtttgatg cgatggtcga taaaactgca atcaaagtga tcatgactta
    10201 cgactttgat gaataactac tctcctgaag gaacgttatc gcatggaaca gataacaaaa
    10261 ccgcattgtg gtgcacggct ggatcgctta ccggattgcc gctggcattc gtcaatgttt
    10321 gccatcgtcg cgtttggctt gcttgtctgc tggagtaatg ccgttggtgg cttgatcctc
    10381 gcgcagctga aagcgttggg ctggacagat aattccacca ctgccacatt ctcagcaatc
    10441 acgaccgccg gaatgtttct cggtgcgctg gtgggcggca tcatcggtga caaaaccggt
    10501 cgcagaaatg cgtttatcct ctatgaggcc attcatattg cttcgatggt tgtcggtgct
    10561 ttctcaccga atatggattt ccttattgcc tgccgttttg tgatgggcgt tgggctggga
    10621 gctttactgg ttacactgtt tgctggtttc accgaatata tgcccggaag aaatcgcgga
    10681 acgtggtcca gtcgggtttc ctttattggt aactggtcat atccgctctg ttcattgatt
    10741 gcgatgggac tcacgccgct gattagtgca gagtggaact ggcgggtaca actgcttatc
    10801 cctgcaatat tgtcacttat cgctacggcg ctggcctggc gctactttcc tgaatccccg
    10861 cgctggctgg aatcgcgcgg acgttatcag gaagccgaga aagtcatgcg gagtatagaa
    10921 gaaggcgtca tacgccagac gggtaaacct ttgccgcctg tggttattgc tgatgacggt
    10981 aaagcaccac aagcggtgcc gtattcagcc ttactgacag gagtattact gaaacgcgtg
    11041 atattaggct cttgtgtgct gattgccatg aacgttgtgc agtacacact aattaactgg
    11101 ttgccaacga tattcatgac ccagggaatt aatttaaaag actcgattgt tttaaatacc
    11161 atgagtatgt ttggcgcgcc atttggtatt tttattgcca tgctggtgat ggataaaatt
    11221 ccgcgtaaaa caatgggtgt ggggctatta atcctgattg cggtgctcgg atatatctat
    11281 tcactgcaaa ccagtatgtt gctcataacg ctgattggtt tcttcctgat tactttcgtc
    11341 tatatgtacg tttgctatgc ctcggcagtg tatgtccctg agatctggcc gacagaggcc
    11401 aaactccgtg gctccggtct ggcgaatgcg gtagggcgaa tcagtggtat tgccgcgcct
    11461 tatgccgttg cagtgctgct cagtagttat ggcgtaacgg gagtctttat tcttctgggg
    11521 gctgtttcaa ttattgtcgc aattgctatc gccaccattg gaattgaaac caaaggtgtc
    11581 tccgttgaaa gtttaagtat tgatgcagta gccaataaat aatactggtg gattcaaaat
    11641 gaaaaattca aaagcaatat tgcaggtgcc gggcacaatg aaaattattt cagcagaaat
    11701 acctgtgcct aaagaagatg aagttttgat taaagtggaa tatgtcggta tttgtggttc
    11761 agatgtacat ggttttgaat caggcccgtt tattccgcct aaagacccaa atcaagaaat
    11821 tggcctgggt catgaatgcg ccgggacggt tgtggctgtg ggaagccgcg tgcgcaaatt
    11881 taaaccgggg gatcgggtaa atatcgaacc tggcgttcct tgcggtcact gtcgttactg
    11941 tctggaaggc aaatataaca tttgcccgga cgttgatttt atggcgacac aacccaacta
    12001 ccgcggcgca ttaacgcact atctgtgtca tccggagagc tttacttaca aactgccaga
    12061 caatatggac acgatggaag gggcgctggt ggagcctgcc gcagtcggga tgcatgccgc
    12121 gatgctggca gatgttaaac ctggtaagaa gataattatt ctgggagcgg gttgtattgg
    12181 tttgatgacg ttgcaagcgt gcaaatgcct gggagcaacg gaaattgccg tcgttgatgt
    12241 gctggaaaaa cgtctgacaa tggcggaaca gctcggcgcg acagtggtta ttaacggcgg
    12301 aaaagaagac actattgcac gctgtcagca atttaccgaa gacatgggcg cagatattgt
    12361 ttttgaaaca gcgggttctg cggtcaccgt taaacaggca ccttatctgg tcatgcgcgg
    12421 cggtaaaatt atgattgttg gtactgtacc cggcgattcg gcaatcaatt tcctcaaaat
    12481 caatcgcgaa gtcactatcc agacggtatt ccgctatgcc aatcgttatc cggtcacgat
    12541 tgaagctatt tcttcagggc gattcgatgt gaaatcgatg gtgacgcata tttacgatta
    12601 tcgggatgta caacaggcat ttgaagagtc agttaacaac aaacgcgaca ttattaaagg
    12661 cgttattaaa attagcgatt aattaatacg ggagaacaga acatgctcgc agatatcaga
    12721 tattgggaaa acgatgccac aaataaacat tatgcaattg cccattttaa cgtatggaat
    12781 gcagaaatgt tgatgggcgt tatcgacgca gccgaagaag cgaaatcacc ggttattatt
    12841 tcttttggca caggttttgt cggcaacacc tcatttgaag atttctctca catgatggta
    12901 tcaatggcac aaaaagcaac ggtgccggta ataactcatt gggatcatgg tcggagtatg
    12961 gagattattc ataacgcctg gactcatggt atgaattcat taatgcgtga tgcttctgca
    13021 tttgatttcg aagaaaatat tcgtttaacc aaagaggctg ttgatttctt ccatccgctg
    13081 ggtattccgg tagaggcgga attagggcat gttggtaatg aaaccgttta tgaggaagcg
    13141 ctggcgggct atcactatac cgatcctgac caggcggctg aatttgttga acgtacgggc
    13201 tgtgactcat tggctgtcgc catcggcaac cagcatgggg tttatacgtc agagccacaa
    13261 ttgaactttg aggtcgtcaa acgcgtacgc gatgctgttt ctgttccgct ggttttgcat
    13321 ggcgcatcag ggatcagtga tgccgacatt aaaactgcaa tttcgttggg tattgcgaaa
    13381 atcaacattc atacggagct ctgtcaggcc gcaatggtag cagtgaaaga aaaccaggat
    13441 cagcctttcc tgcatttaga acgtgaagtg cgtaaagctg taaaagaacg ggcattggat
    13501 aaaattaaac tgttcggttc tgatggcaaa gcggaataat aatatggata atctcgacgt
    13561 tatttgtata ggtgccgcta ttgttgatat tccattgcaa ccggtcagta aaaatatctt
    13621 tgatgtggat tcttatcctc ttgaaagaat cgcaatgacc accgggggtg atgcaataaa
    13681 tgaagcaaca attatttctc gtctgggcca tcgcacagca ttaatgagtc gtattggtaa
    13741 agatgccgca gggcaattta ttctcgacca ttgccgcaaa gagaatattg atattcaaag
    13801 cctgaagcag gacgtcagca tagatacctc tattaacgtt ggactggtga cggaggatgg
    13861 cgagcggacc tttgtcacaa accgtaacgg cagtctgtgg aaattgaata ttgacgatgt
    13921 tgattttgcg cggttttctc aggcgaaatt attatcactg gccagtattt tcaacagtcc
    13981 tctattggat ggtagagcgc taacagaaat ttttacgcaa gctaaaaccc ggcagatgat
    14041 tatctgtgcc gatatgatca aaccgcggtt gaatgaaacg ctgaatgata tttgcgaagc
    14101 attgagttat gtcgattatc tgtttcctaa ttttgccgag gcaaaattac tcaccgggaa
    14161 agagacactg gatgaaattg ctgactgctt tcttgcgtgc ggcgtaaaaa cggtggtgat
    14221 taaaacgggt aaagacggct gctttatcaa gcgtggtgac atgacgatga aggtgccggc
    14281 ggtcgcagga ataaccgcta tcgacaccat tggtgcgggc gataactttg cttcaggttt
    14341 tattgcagca ctgttagaag gtaaaaatct gcgtgaatgc gcacgctttg ccaatgcaac
    14401 ggcggctatc tcggttctaa gcatcggcgc caccactggt gtaaaaaaca gaaagctggt
    14461 cgaacaattg ctggaagaat acgaaggata atgaaggcaa atgaaaaaga tacctttagg
    14521 cacaacggat ataacgcttt cgcgaatggg gttggggaca tgggccattg gcggcggtcc
    14581 tgcatggaat ggcgatctcg atcgacaaat atgcattgat acgattcttg aagcccatcg
    14641 ttgcggcatt aacctgattg atactgcgcc aggatataac tttggcaata gtgaagttat
    14701 cgttggtcag gcgttaaaaa aactgccccg tgaacaggtt gtagtagaaa ccaaatgcgg
    14761 cattgtctgg gaacgaaaag ggagtttatt caacaaagtt ggcgaacggc agttgtataa
    14821 aaacctttct ccggaatcta tccgcgaaga ggtagaagcc agcttgcaac gtctgggtat
    14881 tgattacatc gatatctaca tgacgcactg gcagtcggtg ccgccatttt ttacgccgat
    14941 agctgaaact gtcgcagtgc ttaatgcgtt aaaggccgaa gggaaaattc gcgctatagg
    15001 cgctgctaac gtcgatgctg accatatccg cgagtatctg caacatggtg aactggatat
    15061 tattcaggcg aaatacagta tcctcgaccg ggcaatggaa aacgaactgc tgccgctatg
    15121 tcgtgataat ggcattgtgg tacaggtcta ctctccgcta gagcagggat tgttgaccgg
    15181 gaccatcacg cgtgattacg ttccgggcgg cgctcgggca aataaagtct ggttccagcg
    15241 tgaaaacatg ctgaaagtga ttgatatgct tgaacagtgg cagccactct gtgctcgtta
    15301 tcagtgcaca attcccactc tggcactggc gtggatatta aaacagagtg atttaatctc
    15361 cattcttagt ggggctactg cgccggaaca ggtacgtgaa aatgtcgcgg cactgaatat
    15421 caacttatcg gatgcagacg caacattgat gagggaaatg gcagaggccc tggagcgtta
    15481 aatactcaac acgattcacc tctgtttctg tgaaaactgt gatgaaaatc tgtgattcag
    15541 acaggttatt ttgaaagtaa acatcggtta tgcgataatt gcgctaatgt gatgtaaaaa
    15601 gatacagggg tgtaatcgtg gcggcaaaag acaggattca ggcaattaag caaatggttg
    15661 ccaacgataa aaaggtgaca gtctcaaatt tgagtgggat ttttcaggta accgaagaaa
    15721 ccattcgccg cgatcttgag aagctggaag atgaaggctt tttgaccaga acctatggtg
    15781 gtgctgtttt aaatacagcg atgttgacgg agaatatcca tttttataag cgtgcttcat
    15841 cgttttatga agagaagcag cttattgcac gcaaggcact accctttatc gacaataaaa
    15901 ccaccatggc agccgattcc agtagtaccg taatggaatt gctcaaattg ttacaggacc
    15961 gtagtgacct gacgttgcta actaactcgg cggaagcgat tcatgttctg gctcaatcag
    16021 aaattaaagt cgtttcaacg gggggggaac taaacaaaaa tacactttcc ctgcaaggaa
    16081 gaattactaa agagatcatc agccgctacc atgtcgatat catggtaatg agttgtaaag
    16141 gtcttgatat taacagcggc gcgctggact ctaacgaagc ggaagctgaa atcaaaaaga
    16201 caatgatccg tcaggcgaca gaagttgcgt tattggttga tcactctaag tttgatcgca
    16261 aagcttttgt ccagttagct gactttagtc atattaatta cataataact gataaatcac
    16321 cgggtgcaga gtggatagca ttttgcaaag acaataatat ccagctggtg tggtaattaa
    16381 aggacacgtt gtcaatatta tttgttcctg cgcctggaaa taaaatgatg ataaagatgc
    16441 ggcaagtgaa tttgccgcaa ttgctacctt ttttagcaaa tccgaggcac ccaatggaac
    16501 aatatgatca aattggcgca agactggacc gcttgccttt ggcccggttt cattatcgta
    16561 tatttggtat aataagcttt agtctgttat taacgggttt tttgagttac tccggtaatg
    16621 tcgtcttagc aaagctggta agcaatggat ggtcaaataa tttcctcaat gccgccttta
    16681 cctcggcatt aatgtttggt tatttcatcg gctctcttac cggtgggttt attggtgact
    16741 actttgggcg gcgcagggcg tttcgcataa atcttctcat cgtcggtatt gcggcaacag
    16801 gggccgcttt tgtccctgat atgtactggc tcatcttctt tcgcttcctg atgggaacag
    16861 gaatgggggc gctgattatg gttggctatg cctcatttac ggagtttatc cccgcgacgg
    16921 tgcgtggaaa atggtccgcg cggctctcat ttgttggtaa ctggtcgccc atgctctctg
    16981 cggcgatagg cgtggtggtt atcgcttttt ttagttggcg gataatgttt ctgctgggcg
    17041 gtattggcat actgttagcg tggtttctct caggtaaata ctttattgag tcgccacgat
    17101 ggctggcagg gaaagggcaa atcgcaggtg cagaaagcca acttcgtgaa gtagagcagc
    17161 aaattgaaag agagaagagt attcgtttac ccccgcttac tttgaaccag agcaacagca
    17221 aggttaaggt aatcaagggt accttctggc tgttgtttaa aggggaaatg ttacgacgta
    17281 cattagtcgc gattactgtt ttaattgcaa tgaacatttc gctttatacc atcaccgtat
    17341 ggataccgac catatttgtt aactccggca ttgatgttga taaatcaata ttaatgaccg
    17401 ctgttattat gattggcgct ccggtaggga tatttattgc ggcattaatt attgatcatt
    17461 ttcctcgtcg attatttggc tctgccttac ttattattat tgctgtgtta ggctatatct
    17521 attcaattca gactacagag tgggcgattt taatctatgg cctggtgatg atcttctttt
    17581 tatacatgta tgtgtgcttc gcgtcggcgg tttatatccc ggaactttgg ccaacgcatt
    17641 tacgcctgcg tggttcgggt ttcgttaatg ccgtcggacg gatcgtcgca gtcttcacgc
    17701 cctatggcgt tgcggcatta ttaacacatt atgggtcgat cacggtgttt atggtgcttg
    17761 gtgtcatgtt agtgctctgt gcgctggttc tctccatttt tggcatcgaa acgcggaagg
    17821 tgtcgctgga agagatttct gaggtgaatt aaatctttcg ttgatggcgt caattaccag
    17881 atgtatcact gatgtgtttt atctggccta caatgccagt acagaattgt aggccagcgt
    17941 aaaattactc ctgcgtttct tcccagtctg ccagcgtata tagcgttgcc ccagctgctg
    18001 acatctccat aaacgcgtgc gcactgtcct gtggctggat atttacgcca cgacaaccat
    18061 cggtaatcac gttcacctta tagcctaact gtaacgcgtc cagcacggta aacttaacgc
    18121 aatagtcagt agccagaccc ataacgatca attcattgat tacatgagcg cgcaaccagt
    18181 catcgagcgc agttttctgc cgacggccgt tatcaaaaaa ggcactgtaa ctgtcaacta
    18241 aaggattttc gcctttatgg aataccgctg cgatcgcttt ttgcttcagt aacggatgta
    18301 attgtgcgcc ttcactgttc tgcacacagt gatcgggcca gaaggtttgt ggcaaaccgt
    18361 cgagttggcc cggcgtataa ggctctacac cgtgctgact ggcaaaactg ccgtgattcg
    18421 ccgggtgcca gtcctgactg gcgataaccg cttcaccgcg cgactggcac cagtcaatca
    18481 ggcggttagc gacatccacc gtactgtcac cttccggcac ggcgagcgcg ccaccagcac
    18541 agaaatcatt ttgtaaatcg accagtaaca gggcgcgagg gggcattgca gtctccttaa
    18601 tcatccggcg tcagttcgcc acgcaggttt tggctcatgg ccttgcgaat ggtttcagta
    18661 tcaagttcct ggctaagcag gtaatgcagt ttggttagcg tggcttctac agtcatatct
    18721 gcaccgccaa taacgccggc atgggcgagg gcgttaccag tggcataacc acccatgttc
    18781 actttaccgg acatacattg tgtcaggttg accaccagaa taccgcgatc gctggcttct
    18841 tgtaattcct gcaggaaggc tttgttttgt ggcgcattac ccacgccata tgagcacaga
    18901 atcaatgctt tcacaggttg gcgcagaaaa ttgcgcacga cgtcagcaga aatccccgga
    18961 taaatcgtca ctacgccaat tggttgcggc gtgattggat gaacgatcaa ttccccttca
    19021 ccgtgcgggg cgggtggcgt attcaaacga cgaatatgga tacctgcttc cagtaacgga
    19081 ggaaggtttg gagaggcaaa cgcatcaaaa ccatcggcat gggctttggt ggttcggttg
    19141 ccacgataca gtcgattatt aaaaaagaga gttacttcgt tgataggata attcgccgca
    19201 acgtacaacg cattcagtag attaatttgt ccgtcagagc gtaactcagc cagcgggatt
    19261 tgtgaccctg tcacaatgac cggtttaccg agattctcga gcatgaacga cagtgcagag
    19321 gcggtatacg ccatcgtgtc tgtaccgtgc agaatgacaa aaccatcata atcgtcatag
    19381 tgcgctttaa tatcttcagc aatatgctgc cagtcttctg gcgtcatatc agaagaatcc
    19441 atcaatggcg tatattcatg aatggtgaaa tctggcatct ccgggcgatg gaattccggc
    19501 atcagcgcca gttggcgctg tagatgacct gacaccggaa tataaccctg ctcggaacgc
    19561 tgcatcccga tggtcccgcc cgtgtaggcg acgtaaattg acttcttttg catgatattg
    19621 atatgaatga tatcgaaaga gcaaaagtat atacgtgagg cagggggagc gcacctgata
    19681 ctatgaggat aaggaaatat gtggcgagac agcgggaaga ggattcgtcg acgtaaaaag
    19741 taatgtggcc gacgatctgt cggccactta gtacaagact taacgcacgt tggcgcaggt
    19801 caggcaaaac gcataacggt tttgtgggtc gttaaacgcg gccagcttgt cgctttcact
    19861 tttaacggta gaggctaccg aggccagcgg tgcaggtaac atggcctgga acgcatctgg
    19921 caacattgcc cggatagaac cagacatgtt gtccatcact ttgtcgaaga aggtcggttc
    19981 atcaacgtag tattccagat gccactgttt cacttttgcc agctctgctg ctttggcgac
    20041 cgcatcatcg aaatccccga gactatcgac cagcccgtta gcttttgcat cctgaccggt
    20101 ccagacgtga ccctgggcga ttttatcaat ctgctccggc gttgaatgac gcgcatcagc
    20161 aaccagcgtg ataaagcgtt tatagccatt ctcaatgctt aactgcatca tctgctgcgc
    20221 ttccggcggc agtgccttgg tgatagaaac atctgccagc ggtgaagttg agacaccatc
    20281 agtatgaaca ccaatcgaat ccagactatt ttctacggtg gtgatcacgc cgaagatacc
    20341 gatagaaccg gtcagggtgc tggggttagc cacaatgtaa ttagctggtg tggaaatcca
    20401 gtaaccacca gatgccgcca tgccgcccat cgatacaacc acaggcttac ccgctgcccg
    20461 ggctgctgcc agttcagcgc gaatcacttc agacgcggta acgctgccgc cgggactatt
    20521 aacacgcaga acaatcgctt tcactttcgg gtcaaggcga gcgtcgcgga tttgtgccgc
    20581 agtggtatca ccgccaacat tcccctgagt ttcctcgcca tccataattg cgccattagc
    20641 gaagacgaca ccgatactgt caccagtatc tgccggcgtt ttcaatgcgt aatcgtaata
    20701 actgatggcg cgataatttt tatcagtttt actccagccg aactctttgg tcagagtttt
    20761 ttcgatttcg gcactcgatg ccagtgcatc gaccagcttg ttttccagcg catatttcgc
    20821 ggtatcgcca ccggttttgg ttaaaccctc aagcaaccct tgcgcgccag ggaatacctg
    20881 ctgagcaggg agctggcggt tagcggcaac agtattcaga tagttttgcc acagctcacc
    20941 aatccagcgg ctgtcagctt cgcgggctgc cggcgacata tcatcacgaa taaacggttc
    21001 aacggcagat ttatacgtac ccacgcggaa cacatgggtg gaaactttca gcttatccag
    21061 taacgatttg tagtacagac cgttggtggc aaagccgtgc agatcaacca cgccttgcgg
    21121 agacagccaa attttattgg cgaaactggc gagataatat tgcccctggc tgtagttctc
    21181 gccaacggca taaaccggtt tcccgctgtc acgaaactct ttcagagctt tgccgatgta
    21241 ctgcatagac ggctggtcgc cgcctgcgaa gtttttcaga tccatcacaa taccggtgat
    21301 attgcggtcg tccttcgcct ggcgaatggt gttgacgata tcaaacagtg agttttcctg
    21361 cagacgatcg gaactggcac caagcagctg gcggcttaat ttactaaacc gctgagaact
    21421 gtcgggttta tcgacgatca caccagaaat gtccagcagc agtgcgccac gactggccgt
    21481 ttctttcgaa tcaccaccac tgacctgcat ccagatcccc acaccaacca gcacgagaaa
    21541 aataaagaac aggttaagta ccatttcacg gacgaaattc agcagacgcc acgtccattt
    21601 aaaaaatccg gcaataaatc gccaaagggt tcgcatgtat tctcccaact taaggtcaca
    21661 cctgtttcag gcgcaataga tgtggttatc gtaatgaccc aaccggcact tgtcagcagg
    21721 aattgcggac tgggctgtaa caaaatccac tctcgtgtta attttgtgaa taaatatcat
    21781 gacagaagtt aatcaaatgg atgcactcga actattgatc aatcgccgta gcgcctcccg
    21841 cctggctgaa cccgcgccaa cgggtgaaca actgcaaaac atcctgcgtg cgggtatgcg
    21901 tgcgccggac cataagtcca tgcaaccgtg gcatttcttt gtgattgaag gggaagggcg
    21961 cgagcgtttc agcgccgtac tggagcaggg ggcgattgct gccggtagtg ataacaaagc
    22021 aatcgacaaa gcgcgtaatg cgccgttccg cgcaccgctc atcataacgg tggtggcgaa
    22081 atgcgaagaa aatcataaag tcccgcgctg ggaacaggaa atgtctgcgg gatgcgcggt
    22141 catggcgatg caaatggcag cagttgccca ggggtttggc ggcatctggc gcagtggtgc
    22201 attaactgaa agtccggtag tgcgtgaagc attcggttgc cgtgagcagg ataaaattgt
    22261 cggttttctc tacctcggta cgccgcagtt gaaagcatct acgtcgataa acgtcccgga
    22321 cccgacgccg tttgtgactt atttctgata attgtcgcga aactgtctgg attgtgagca
    22381 aaggcgcagg aattcagaca ctctcactta tcacttcatg gaatgagggt taccataccc
    22441 ggattgcatt gacaggagat gtccatgagc gagaactcga ttcgtttgac ccaatacagc
    22501 cacggcgctg gttgcggctg taaaatttcc ccaaaagtgt tggaaaccat cctgcacagt
    22561 gagcaggcga agtttgttga tccgaatttg cttgtgggta atgaaacccg cgacgatgcg
    22621 gcggtgtacg atctgggcaa tggcaccagc gttatcagca ccaccgactt ctttatgccg
    22681 atcgttgata atcctttcga ttttggccgc attgcggcga ctaacgccat cagcgatatc
    22741 tttgctatgg gcggcaaacc gattatggcg attgcgatcc tcggctggcc gattaacaaa
    22801 ctttccccgg aaattgcccg cgaagtgacc gaaggtggac gctatgcatg ccgtcaggcg
    22861 gggattgcgc tggctggcgg tcactccatc gatgcgccgg agccgatttt tggtctggcg
    22921 gtaacgggga tcgtaccgac cgagcgggtg aagaaaaaca gtaccgcaca agccggatgc
    22981 aaactgttcc tgacgaaacc gctggggatt ggcgttctta ccacggctga gaaaaaatca
    23041 ctgctgaaac cagaacatca gggactggcg acggaagtga tgtgccggat gaacatcgca
    23101 ggcgcgtcct ttgccaacat cgaaggcgta aaagcgatga ccgacgttac gggttttggt
    23161 ttgctggggc atttaagcga aatgtgtcag ggcgcaggtg tgcaggcgcg cgtcgactat
    23221 gacgcgatcc cgaaattgcc tggggttgaa gagtacatta agttgggcgc agtgcctggc
    23281 ggcaccgaac gtaactttgc cagctacggt catctgatgg gtgaaatgcc gcgtgaagtg
    23341 cgcgatctgc tgtgcgatcc gcaaacttct ggcggtttgc tgctggcggt catgccggaa
    23401 gcagaaaatg aggtcaaagc tacagccgcc gagtttggca ttgaactgac ggcgattggc
    23461 gaactggtgc cagcgcgcgg cggtcgtgcc atggttgaga tccgttaatt caatgcggtt
    23521 gtttattgcc gaaaaaccga gtctggcgcg cgccattgct gatgtcctgc ctaaaccgca
    23581 ccggaaaggc gatggcttta tcgagtgcgg taatggtcag gtggtgacct ggtgtatcgg
    23641 tcacctgctt gagcaggcgc agccagacgc ctacgacagc cgctatgcgc gctggaatct
    23701 tgcggatttg ccaattgtcc cggaaaagtg gcaattacag ccccgaccct ccgtgactaa
    23761 acaacttaat gtcatcaaac gcttcctgca tgaagccagc gaaatcgttc acgccggaga
    23821 cccggatcgc gaagggcaac tgctggtgga tgaagtgctg gactacctgc aactggcacc
    23881 ggaaaagcgc cagcaggtac agcgttgttt gataaacgac ctgaacccgc aggcggttga
    23941 gcgggcgatc gatcgtcttc gctccaatag cgagtttgta ccgctgtgcg tttctgcact
    24001 ggcgcgggcg cgggctgact ggctgtacgg cattaatatg acccgtgcgt ataccattct
    24061 cggtcgcaat gccggttatc agggcgtgct ttccgtggga cgcgtgcaga cgcccgtact
    24121 cggactggtg gtgcgccgcg acgaagagat tgaaaacttc gtggcgaaag acttctttga
    24181 agtcaaagca catatcgtga cacctgccga tgagcggttt accgctatct ggcaaccgag
    24241 cgaagcgtgt gaaccgtacc aggatgaaga agggcgcttg ttacatcgtc cactggcgga
    24301 gcatgtggtt aaccgcatta gtggtcaacc agctattgtc accagctata acgataaacg
    24361 ggaatcagaa tccgcgccgc tgcctttttc gctttcggcg ttgcagattg aagcggctaa
    24421 acgttttggt ctgagcgcgc agaacgtgct tgatatctgc cagaagctgt acgaaacgca
    24481 caagctaatc acttatccgc gttccgattg ccgctatttg ccagaagaac attttgccgg
    24541 acgccacgcg gtgatgaatg ctatcagcgt tcatgcaccg gatctgttgc cgcagccagt
    24601 ggtagatcca gatatacgca accgctgttg ggatgacaaa aaggtcgatg cgcaccacgc
    24661 catcattccg accgcacgga gttctgcgat caacctgacg gagaacgaag cgaaggtcta
    24721 taacctgatt gcccgtcagt acttgatgca gttctgcccg gatgcggtgt tccgcaagtg
    24781 tgttatcgaa ctggacattg ccaaaggcaa atttgtcgct aaagcccgtt ttcttgctga
    24841 agcaggctgg cgcacgctgt taggcagcaa agagcgcgat gaagaaaacg acggcacgcc
    24901 gctgcctgtg gtggcgaaag gcgatgaatt gttgtgcgaa aaaggcgaag tggtagagcg
    24961 gcaaacccag ccgccacgcc attttaccga tgcaacactg ctttcggcga tgaccgggat
    25021 cgcacgcttt gtgcaggaca aagatctgaa aaagatcctt cgtgcgaccg atggtttggg
    25081 gacagaagca acccgtgccg ggattattga actgttgttc aagcgtggtt tcctgaccaa
    25141 aaaagggcgc tatatccact ccaccgacgc cggaaaagcg ctattccatt cgctgccaga
    25201 aatggcgacg cgaccggaca tgaccgcgca ctgggaatcg gtgctgacgc aaatcagcga
    25261 aaagcagtgt cgctatcagg actttatgca gccgctggtg gggacgcttt accaactgat
    25321 tgatcaggct cgtagcactc cggtcaggca gttcagagga attgtggcat cgggtggggg
    25381 ggctgttaaa aaagcaccca ataagcgtgt gaaaaaagcg gcaaagccca aaacagataa
    25441 tacggattct gctaattaat tcgttttcca ctcttaaata aagccaatgg tagatattct
    25501 gccattggct ttattggtta tactcgtcat gctttaagtt gcatgtgctg cgtctgcgtt
    25561 cgttcagccc agtcacttac ttatgtaagc tcctcggga
//
LOCUS       DADSYM010000065        25465 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2415
            SAMN08039579-rid11723173.denovo.065, whole genome shotgun sequence.
ACCESSION   DADSYM010000065 DADSYM010000000
VERSION     DADSYM010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039579
            Sequence Read Archive: SRR6362533
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 25465)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25465)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980162.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 03:39:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,484
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 4,347
            CDSs (with protein)               :: 4,347
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 43 of 85
            Pseudo Genes (incomplete)         :: 37 of 85
            Pseudo Genes (internal stop)      :: 19 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25465
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039579-rid11723173.denovo.065"
                     /strain="LGE2415"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8302 N 95.9628 W"
                     /collection_date="2015-08-24"
     gene            complement(28..1887)
                     /gene="gss"
                     /locus_tag="J1Y83_002244"
     CDS             complement(28..1887)
                     /gene="gss"
                     /locus_tag="J1Y83_002244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutathionylspermidine
                     amidase/synthase"
                     /protein_id="HBA7630391.1"
                     /translation="MSKGTTSQDAPFGTLLGYAPGGVAIYSSDYSSLDPQEYEDDAVF
                     RSYIDDEYMGHKWQCVEFARRFLFLNYGVVFTDVGMAWEIFSLRFLREVVNDNILPLQ
                     AFPNGSPRAPVAGALLIWDKGGEFKDTGHVAIITQLHGNKVRIAEQNVIHSPLPQGQQ
                     WTRELEMVVENGGYTLKDTFDDTTILGWMIQTEDTEYSLPQPEIAGELLKISGARLEN
                     KGQFDGKWLDEKDPLQNAYVQANGQVINQDPYHYYTITESAEQELIKATNELHLMYLH
                     ATDKVLKDDNLLALFDIPKILWPRLRLSWQRRRHHMITGRMDFCMDERGLKVYEYNAD
                     SASCHTEAGLILERWAEQGYKGNGFNPAEGLINELAGAWKHSRARPFVHIMQDKDIEE
                     NYHAQFMEQALHQAGFETRILRGLDELGWDAAGQLIDGEGRLVNCVWKTWAWETAFDQ
                     IREVSDREFAAVPIRTGHPQNEVRLIDVLLRPEVLVFEPLWTVIPGNKAILPILWSLF
                     PHHRYLLDTDFTVNDELVKTGYAVKPIAGRCGSNIDLVSHHEEVLDQTSGKFAEQKNI
                     YQQLWCLPKVDGKYIQVCTFTVGGNYGGTCLRGDESLVIKKESDIEPLIVVKE"
     gene            2092..2958
                     /gene="yghU"
                     /locus_tag="J1Y83_002245"
     CDS             2092..2958
                     /gene="yghU"
                     /locus_tag="J1Y83_002245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-dependent disulfide-bond
                     oxidoreductase"
                     /protein_id="HBA7630392.1"
                     /translation="MTDNTYQPAKVWTWDKSAGGAFASINRPVSGPTHEKTLPVGKHP
                     LQLYSLGTPNGQKVTIMLEELLALGVTGAEYDAWLIRIGDGDQFSSGFVEVNPNSKIP
                     ALRDHTHNPPIRVFESGSILLYLAEKFGYFLPQDLAKRTETMSWLFWLQGAAPFLGGG
                     FGHFYHYAPVKIEYAINRFTMEAKRLLDVLDKQLAQHKFVAGDEYTIADIAIWPWFGN
                     VVLGGVYDAAEFLDAGSYKHVQRWAKEVGERPAVKRGRIVNRINGPLNEQLHERHDAS
                     DFETNTEDKRQG"
     gene            complement(3100..3348)
                     /gene="hybG"
                     /locus_tag="J1Y83_002246"
     CDS             complement(3100..3348)
                     /gene="hybG"
                     /locus_tag="J1Y83_002246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation factor HybG"
                     /protein_id="HBA7630393.1"
                     /translation="MCIGVPGQVLAVGEDIHQLAQVEVCGIKRDVNIALICEGNPADL
                     LGQWVLVHVGFAMSIIDEDEAKATLDALRQMDYDITSA"
     gene            complement(3361..3702)
                     /gene="hypA"
                     /locus_tag="J1Y83_002247"
     CDS             complement(3361..3702)
                     /gene="hypA"
                     /locus_tag="J1Y83_002247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HBA7630394.1"
                     /translation="MHELSLCQSAVEIIQRQAEQHDVKRVTAVWLEIGALSCVEESAV
                     RFSFEIVCHGTVAQGCDLHIVYKPAQAWCWDCSQVVEIHQHDAQCPICHGERLRVDTG
                     DSLIVKSIEVE"
     gene            complement(3695..4183)
                     /gene="hybE"
                     /locus_tag="J1Y83_002248"
     CDS             complement(3695..4183)
                     /gene="hybE"
                     /locus_tag="J1Y83_002248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase-2 assembly chaperone"
                     /protein_id="HBA7630395.1"
                     /translation="MTEEIAGFQTSPKAQVQVAFEEIARRSMHDLSFLHPSMPVYVSD
                     FTLFEGQWTGCVITPWMLSAVIFPGPDQLWPLRKVSEKIGLQLPYGTMTFTVGELDGV
                     SQYLSCSLMSPLSHSMSIEEGQRLTDDCARMILSLPVTNPDVPHAGRRALLFGRRSGE
                     NA"
     gene            complement(4176..4670)
                     /locus_tag="J1Y83_002249"
     CDS             complement(4176..4670)
                     /locus_tag="J1Y83_002249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HyaD/HybD family hydrogenase maturation
                     endopeptidase"
                     /protein_id="HBA7630396.1"
                     /translation="MRILVLGVGNILLTDEAIGVRIVEALEQRYILPDYVEILDGGTA
                     GMELLGDMANRDHLIIADAIVSKKNAPGTMMILRDEEVPALFTNKISPHQLGLADVLS
                     ALRFTGEFPKKLTLVGVIPESLEPHIGLTPTVEAMIEPALEQVLAALRESGVEAIPRE
                     ATHD"
     gene            complement(4670..6373)
                     /gene="hybC"
                     /locus_tag="J1Y83_002250"
     CDS             complement(4670..6373)
                     /gene="hybC"
                     /locus_tag="J1Y83_002250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 large subunit"
                     /protein_id="HBA7630397.1"
                     /translation="MSQRITIDPVTRIEGHLRIDCEIENGVVSKAWASGTMWRGMEEI
                     VKNRDPRDAWMIVQRICGVCTTTHALSSVRAAESALNIDVPVNAQYIRNIILAAHTTH
                     DHIVHFYQLSALDWVDITSALQADPAKASEMLKGVSTWHLNSPEEFTKVQNKIKDLVA
                     SGQLGIFANGYWGHPAMKLPPEVNLIAVAHYLQALECQRDANRVVALLGGKTPHIQNL
                     AVGGVANPINLDGLGVLNLERLMYIKSFIDKLSDFVEQVYKVDTAVIAAFYPEWLTRG
                     KGAVNYLSVPEFPTDSKNGSFLFPGGYIENADLSSYRPITSHSDEYLIKGIQESAKHS
                     WYKDEAPQAPWEGTTIPAYDGWSDDGKYSWVKSPTFYGKTVEVGPLANMLVKLAAGRE
                     STQNKLNEIVAIYQKLTGNTLEVAQLHSTLGRIIGRTVHCCELQDILQNQYSALITNI
                     GKGDHTTFVKPNIPATGEFKGVGFLEAPRGMLSHWMVIKDGIISNYQAVVPSTWNSGP
                     RNFNDDVGPYEQSLVGTPVADPNKPLEVVRTIHSFDPCMACAVHVVDADGNEVVSVKV
                     L"
     gene            complement(6370..7548)
                     /gene="hybB"
                     /locus_tag="J1Y83_002251"
     CDS             complement(6370..7548)
                     /gene="hybB"
                     /locus_tag="J1Y83_002251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase cytochrome b subunit"
                     /protein_id="HBA7630398.1"
                     /translation="MSHDPQPLGGKIISKPVMIFGPLIVICMLLIVKRLVFGLGSVSD
                     LNGGFPWGVWIAFDLLIGTGFACGGWALAWAVYVFNRGQYHPLVRPALLASLFGYSLG
                     GLSITIDVGRYWNLPYFYIPGHFNVNSVLFETAVCMTIYIGVMALEFAPALFERLGWK
                     VSLKRLNKVMFFIIALGALLPTMHQSSMGSLMISAGYKVHPLWQSYEMLPLFSLLTAF
                     IMGFSIVIFEGSLVQAGLRGNGPDEKSLFVKLTNTISVLLAIFIVLRFGELIYRDKLS
                     LAFAGDFYSVMFWIEVLLMLFPLVVLRVAKLRNDSRMLFLSALSALLGCATWRLTYSL
                     VAFNPGGGYAYFPTWEELLISIGFVAIEICAYIVLIRLLPILPPLKQNDHNRHEASKA
                     "
     gene            complement(7538..8524)
                     /gene="hybA"
                     /locus_tag="J1Y83_002252"
     CDS             complement(7538..8524)
                     /gene="hybA"
                     /locus_tag="J1Y83_002252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 operon protein HybA"
                     /protein_id="HBA7630399.1"
                     /translation="MNRRNFIKAASCGALLTGALPSVSHAVAENRPPIPGSLGMLYDS
                     TLCVGCQACVTKCQDINFPERNPQGEQTWSNNDKLSPYTNNIIQVWTSGTGVNKDQEE
                     NGYAYIKKQCMHCVDPNCVSVCPVSALKKDPKTGIVHYDKDVCTGCRYCMVACPYNVP
                     KYDYNNPFGALHKCELCNQKGVERLDKGGLPGCVEVCPAGAVIFGTREELMAEAKKRL
                     ALKPGSEYHYPRQTLKSGDTYLHTVPKYYPHLYGEKEGGGTQVLVLTGVPYENLDLPK
                     LDDLSTGARSEHVQHTLYKGMMLPLAVLAGLTVLVRRNTKNDHHDGGDDHES"
     gene            complement(8527..9645)
                     /gene="hybO"
                     /locus_tag="J1Y83_002253"
     CDS             complement(8527..9645)
                     /gene="hybO"
                     /locus_tag="J1Y83_002253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 small subunit"
                     /protein_id="HBA7630400.1"
                     /translation="MTGDNTLIHSHGINRRDFMKLCAALAATMGLSSKAAAEMAESVT
                     NPQRPPVIWIGAQECTGCTESLLRATHPTVENLVLETISLEYHEVLSAAFGHQVEENK
                     HNALEKYKGQYVLVVDGSIPLKDNGIYCMVAGEPIVDHIRKAAEGAAAIIAIGSCSAW
                     GGVAAAGVNPTGAVSLQEVLPGKTVINIPGCPPNPHNFLATVAHIITYGKPPKLDDKN
                     RPTFAYGRLIHEHCERRPHFDAGRFAKEFGDEGHREGWCLYHLGCKGPETYGNCSTLQ
                     FCDVGGVWPVAIGHPCYGCNEEGIGFHKGIHQLANVENQTPRSQKPDVNAKEGGNVSA
                     GAIGLLGGVVGLVAGVSVMAVRELGRQQKKDNADSRGE"
     gene            complement(9834..10121)
                     /locus_tag="J1Y83_002254"
     CDS             complement(9834..10121)
                     /locus_tag="J1Y83_002254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2623 domain-containing protein"
                     /protein_id="HBA7630401.1"
                     /translation="MNNHFGKGLMAGLKATHADSAVNVTKFCADYKRGFVLGYSHRMY
                     EKTGDRQLSAWEAGILTRRYGLDKEMVMDFFRENNSCSTLRFFMAGYRLEN"
     gene            complement(10240..11127)
                     /locus_tag="J1Y83_002255"
     CDS             complement(10240..11127)
                     /locus_tag="J1Y83_002255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311911.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HBA7630402.1"
                     /translation="MPRLTAKAFPQELLDYYDYYAHGKISKREFLNLAAKYAVGGMTA
                     LALFDLLKPNYALATQVEFTDPEIVAEYITYPSPNGHGEVRGYLVKPAKMSGKTPAVV
                     VVHENRGLNPYIEDVTRRVAKEGYIALAPDGLSSVGGYPGNDDKGRELQQQVDPTKLM
                     NDFFAAIEFMQRYPQATGKVGISGFCYGGGVSNAAAVAYPELACAVPFYGRQAPTADV
                     AKIEAPLLLHYAELDSRINEGWPAYEAALKANNKVYEAYIYPGVNHGFHNDSTPRYDK
                     SAADLAWQRTLKWFDKYLS"
     gene            11284..12324
                     /gene="gpr"
                     /locus_tag="J1Y83_002256"
     CDS             11284..12324
                     /gene="gpr"
                     /locus_tag="J1Y83_002256"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-glyceraldehyde 3-phosphate reductase"
                     /protein_id="HBA7630403.1"
                     /translation="MVWLANPERYGRMQYRYCGKSGLRLPALSLGLWHNFGHVNALES
                     QRAILRKAFDLGITHFDLANNYGPPPGSAEENFGRLLREDFAAYRDELIISTKAGYDM
                     WPGPYGSGGSRKYLLASLDQSLKRMGLEYVDIFYSHRVDENTPMEETASALAHAVQSG
                     KALYVGISSYSPERTQKMVELLREWKIPLLIHQPSYNLLNRWVDKSGLLDTLQNNGVG
                     CIAFTPLAQGLLTGKYLNGIPQDSRMHREGNKVRGLTPKMLTEANLNSLRLLNEMAQQ
                     RGQSMAQMALSWLLKDERVTSVLIGASRAEQLEENVQALNNLTFSTEELAQIDQHIAD
                     GELNLWQASSDK"
     gene            complement(12364..12858)
                     /locus_tag="J1Y83_002257"
     CDS             complement(12364..12858)
                     /locus_tag="J1Y83_002257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00645 family protein"
                     /protein_id="HBA7630404.1"
                     /translation="MERFLENAMYASRWLLAPVYFGLSLALVALALKFFQEIIHVLPN
                     IFSMAESDLILVLLSLVDMTLVGGLLVMVMFSGYENFVSQLDISENKEKLNWLGKMDA
                     TSLKNKVAASIVAISSIHLLRVFMDAKNVPDNKLMWYVIIHLTFVLSAFVMGYLDRLT
                     RHNH"
     gene            13049..13933
                     /gene="yghA"
                     /locus_tag="J1Y83_002258"
     CDS             13049..13933
                     /gene="yghA"
                     /locus_tag="J1Y83_002258"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(+)-dependent aldehyde reductase"
                     /protein_id="HBA7630405.1"
                     /translation="MSHLKDPTTQYYTGEYPKQKQPTPGIQAKMTPVPDCGEKTYVGS
                     GRLKDRKALVTGGDSGIGRAAAIAYAREGADVAISYLPVEEEDAQDVKKIIEECGRKA
                     VLLPGDLSDEKFARSLVHEAHKALGGLDIMALVAGKQVAIPDIADLTSEQFQKTFAIN
                     VFALFWLTQEAIPLLPKGASIITTSSIQAYQPSPHLLDYAATKAAILNYSRGLAKQVA
                     EKGIRVNIVAPGPIWTALQISGGQTQDKIPQFGQKTPMKRAGQPAELAPVYVYLASQE
                     SSYVTAEVHGVCGGEHLG"
     gene            complement(13972..14397)
                     /gene="exbD"
                     /locus_tag="J1Y83_002259"
     CDS             complement(13972..14397)
                     /gene="exbD"
                     /locus_tag="J1Y83_002259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB system transport protein ExbD"
                     /protein_id="HBA7630406.1"
                     /translation="MAMHLNENLDDNGEMHDINVTPFIDVMLVLLIIFMVAAPLATVD
                     VKVNLPASTSTPQPRPEKPVYLSVKADNSMFIGNDPVTDETMITALNALTEGKKDTTI
                     FFRADKTVDYETLMKVMDTLHQAGYLKIGLVGEETAKAK"
     gene            complement(14404..15138)
                     /locus_tag="J1Y83_002260"
     CDS             complement(14404..15138)
                     /locus_tag="J1Y83_002260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbB"
                     /protein_id="HBA7630407.1"
                     /translation="MGNNLMQTDLSVWGMYQHADIVVKCVMIGLILASVVTWAIFFSK
                     SVEFFNQKRRLKREQQLLAEARSLNQANDIAADFGSKSLSLHLLNEAQNELELSEGSD
                     DNEGIKERTSFRLERRVAAVGRQMGRGNGYLATIGAISPFVGLFGTVWGIMNSFIGIA
                     QTQTTNLAVVAPGIAEALLATAIGLVAAIPAVVIYNVFARQIGGFKAMLGDVAAQVLL
                     LQSRDLDLEASAAAHPVRVAQKLRAG"
     gene            15390..16577
                     /gene="metC"
                     /locus_tag="J1Y83_002261"
     CDS             15390..16577
                     /gene="metC"
                     /locus_tag="J1Y83_002261"
                     /EC_number="4.4.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine beta-lyase"
                     /protein_id="HBA7630408.1"
                     /translation="MADKKLDTQLVNAGRSKKYTLGAVNSVIQRASSLVFDSVEAKKH
                     ATRNRANGELFYGRRGTLTHFSLQQAMCELEGGAGCVLFPCGAAAVANSILAFVEQGD
                     HVLMTNTAYEPSQDFCSKILSKLGVTTSWFDPLIGADIVKHLQPNTKIVFLESPGSIT
                     MEVHDVPAIVAAVRSVVPDAIIMIDNTWAAGVLFKALDFGIDVSIQAATKYLVGHSDA
                     MIGTAVCNARCWEQLRENAYLMGQMVDADTAYITSRGLRTLGVRLRQHHESSLKVAEW
                     LAEHPQVARVNHPALPGSKGHEFWKRDFTGSSGLFSFVLKKKLNDEELANYLDNFSLF
                     SMAYSWGGYESLILANQPEHIAAIRPQGEIDFSGTLIRLHIGLEDVDDLIADLDAGFA
                     RIV"
     gene            16717..17376
                     /locus_tag="J1Y83_002262"
     CDS             16717..17376
                     /locus_tag="J1Y83_002262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HBA7630409.1"
                     /translation="MAVIQDIIAALWQHDFAALADPHIVSVVYFVMFATLFLENGLLP
                     ASFLPGDSLLILAGALIAQGVMDFLPTIAILTAAASLGCWLSYIQGRWLGNTKTVKGW
                     LAQLPAKYHQRATCMFDRHGLLALLAGRFLAFVRTLLPTMAGISGLPNRRFQFFNWLS
                     GLLWVSVVTSFGYALSMIPFVKRHEDQVMTFLMILPIALLTAGLLGTLFVVIKKKYCN
                     A"
     gene            complement(17416..18315)
                     /locus_tag="J1Y83_002263"
     CDS             complement(17416..18315)
                     /locus_tag="J1Y83_002263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003024513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HBA7630410.1"
                     /translation="MKREEICRLLADKVNKLKNKENSLSELLPDVRLLYGETPFARTP
                     VMYEPGIIILFSGHKIGYINERVFRYDANEYLLLTVPLPFECETYATSEVPLAGLRLN
                     VDILQLQELLMDIGEDEHFQPSMAASGINSATLSEEILCAAERLLDVMERPLDARILG
                     KQIIREILYYVLTGPCGGALLALVSRQTHFSLISRVLKRIENKYTENLSVEQLAAEAN
                     MSVSAFHHNFKSVTSTSPLQYLKNYRLHKARMMIIHDGMKASAAAMRVGYESASQFSR
                     EFKRYFGVTPGEDAARMRAMQGN"
     gene            18509..19672
                     /gene="yqhD"
                     /locus_tag="J1Y83_002264"
     CDS             18509..19672
                     /gene="yqhD"
                     /locus_tag="J1Y83_002264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase"
                     /protein_id="HBA7630411.1"
                     /translation="MNNFNLHTPTRILFGKGAIAGLREQIPHDARVLITYGGGSVKKT
                     GVLDQVLDALKGMDVLEFGGIEPNPAYETLMNAVKLVREQKVTFLLAVGGGSVLDGTK
                     FIAAAANYPENIDPWHILQTGGKEIKSAIPMGCVLTLPATGSESNAGAVISRKTTGDK
                     QAFHSAHVQPVFAVLDPVYTYTLPPRQVANGVVDAFVHTVEQYVTKPVDAKIQDRFAE
                     GILLTLIEDGPKALKEPENYDVRANVMWAATQALNGLIGAGVPQDWATHMLGHELTAM
                     HGLDHAQTLAIVLPALWNEKRDTKRAKLLQYAERVWNITEGSDDERIDAAIAATRNFF
                     EQLGVPTHLSDYGLDGSSIPALLKKLEEHGMTQLGENHDITLDVSRRIYEAAR"
     gene            19777..20604
                     /gene="dkgA"
                     /locus_tag="J1Y83_002265"
     CDS             19777..20604
                     /gene="dkgA"
                     /locus_tag="J1Y83_002265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417485.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,5-didehydrogluconate reductase DkgA"
                     /protein_id="HBA7630412.1"
                     /translation="MANPTVIKLQDGNVMPQLGLGVWQASNEEVITAIQKALEVGYRS
                     IDTAAAYKNEEGVGKALKNASVNREELFITTKLWNDDHKRPREALLDSLKKLQLDYID
                     LYLMHWPVPAIDHYVEAWKGMIELQKEGLIKSIGVCNFQIHHLQRLIDETGVTPVINQ
                     IELHPLMQQRQLHAWNATHKIQTESWSPLAQGGKGVFDQKVIRDLADKYGKTPAQIVI
                     RWHLDSGLVVIPKSVTPSRIVENFDVWDFRLDKDELGEIAKLDQGKRLGPDPDQFGG"
     gene            20804..21730
                     /locus_tag="J1Y83_002266"
     CDS             20804..21730
                     /locus_tag="J1Y83_002266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjP/YqhG family protein"
                     /protein_id="HBA7630413.1"
                     /translation="MKIILLFLAALASFTVHAQPPSQTVEQTVRHIYQNYKSDATAPY
                     FGETGERAITSARIQQALTLNDNLTLPGNIGWLDYDPVCDCQDFGDLVLESVAITQTD
                     ADHADAIVRFRIFQDDKEKTSQTLKMVAENGRWVIDDIVSNHGSVLQAVNSENEKTLA
                     AIASLQKEQPEAFVAELFEHIADYSWPWTWVVSDSYRQAVNAFYKTTFKTANNPDEDM
                     QIERQFIYDNPICFGEESLFSRVDEIRVLGKTTDSARIHVRFTLTNGNNEEQELILQR
                     REGKWEIADFIRPNSGSLLKQIEAKTAARLKQ"
     gene            21781..22038
                     /gene="yqhH"
                     /locus_tag="J1Y83_002267"
     CDS             21781..22038
                     /gene="yqhH"
                     /locus_tag="J1Y83_002267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YqhH"
                     /protein_id="HBA7630414.1"
                     /translation="MKTIFTVGAVVLATCLLSGCVNEQKVNQLASNVQTLNAKIARLE
                     QDMKALRPQIYAAKSEANRANTRLDAQDYFDCLRCLRMYAE"
     gene            complement(22080..24299)
                     /locus_tag="J1Y83_002268"
     CDS             complement(22080..24299)
                     /locus_tag="J1Y83_002268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgiQ family radical SAM protein"
                     /protein_id="HBA7630415.1"
                     /translation="MSSISLIQPDRDLFSWPQYWAACFGPAPFLPMSREEMDQLGWDS
                     CDIILVTGDAYVDHPSFGMAICGRMLEAQGFRVGIIAQPDWSSKDDFMRLGKPNLFFG
                     VTAGNMDSMINRYTTDRRLRHDDAYTPDNVAGKRPDRATLVYTQRCKEAWKDVPVILG
                     GIEASLRRTAHYDYWSDTVRRSVLVDSKADMLMFGNGERPLVEVAHRLAMGEPISEIR
                     DVRNTAIIVKEALPGWSGVDSTRLDTPGKIDPIPHPYGEDLPCADNKPVAPKKQEAKA
                     VTVQPPRPKPWEKTYVLLPSFEKVKGDKVLYAHASRILHHETNPGCARALMQKHGDRY
                     VWINPPAIPLSTEEMDSVFALPYKRVPHPAYGNARIPAYEMIRFSVNIMRGCFGGCSF
                     CSITEHEGRIIQSRSEDSIINEIEAIRDTVPGFTGVISDLGGPTANMYMLRCKSPRAE
                     QTCRRLSCVYPDICPHMDTNHEPTINLYRRARDLKGIKKILIASGVRYDIAVEDPRYI
                     KELATHHVGGYLKIAPEHTEEGPLSKMMKPGMGSYDRFKELFDTYSKQAGKEQYLIPY
                     FISAHPGTRDEDMVNLALWLKKHRFRLDQVQNFYPSPLANSTTMYYTGKNPLAKIGYK
                     SEDVFVPKGDKQRRLHKALLRYHDPANWPLIRQALEAMGKKHLIGSRRDCLVPAPTIE
                     EMREARRQNRNTRPALTKHTPMATQRQTPATAKKASSTQSRLQNAGAKKRPKAAVGR"
     gene            24551..25300
                     /locus_tag="J1Y83_002269"
     CDS             24551..25300
                     /locus_tag="J1Y83_002269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000438650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FCD domain-containing protein"
                     /protein_id="HBA7630416.1"
                     /translation="MEQVITKRRYYDIGLQIEELLYSGVFKAGERLPSERELSERFNT
                     SRTTIREAIIMLELKGVLNVKQGSGIFFVDSTDKLNQKSLMPYSEIGPFELLQARQVI
                     ESNITGFAASQISFNELQELKKIIGLQEKAIAAESDKFEDLDHRFHSIIAEATQNRVL
                     IKQAAELWRAVRTENPRWKKLNYKYLHEKHLRLQWLEDHRAIFLALQQKDSELAREAS
                     WRHLENSKNELIKIFKQDASISDFDDFFFAR"
BASE COUNT         6368 a         6766 c         6350 g         5981 t
ORIGIN      
        1 atcagtacgg ggttgctaca acatgtatta ctctttcacc acaattaacg gttcaatatc
       61 actctctttt ttgatgacca gtgattcatc accgcgcaaa cacgtcccac cgtagttgcc
      121 gccaacggtg aaagtacata cctgaatgta tttaccgtcc actttcggca aacaccacag
      181 ttgctgatag atgtttttct gctcggcaaa tttaccactg gtctgatcaa gcacctcttc
      241 atgatggctg acgaggtcga tattgctacc acagcgaccg gcaattggtt tcaccgcgta
      301 gccggttttc accagttcat cattaacagt gaaatcggta tccagcaggt aacgatggtg
      361 cgggaacagc gaccagagga tcggcagaat cgctttgttg ccggggatca ccgtccacag
      421 cggctcaaag accagcactt ccgggcgcag caatacgtcg ataagacgca cttcattttg
      481 cggatgaccg gtacggatcg gcaccgcagc aaactcacgg tcgctaactt cacgaatctg
      541 atcaaacgcg gtttcccacg cccaggtttt ccatacgcag ttaaccagtc gcccttcccc
      601 atcaatcagt tgcccggcag catcccagcc cagttcatcc aacccacgca agatacgcgt
      661 ttcaaagccc gcctgatgca gcgcctgctc cataaactgc gcgtgatagt tttcctcgat
      721 atctttgtcc tgcatgatat ggacaaacgg acgtgcacga ctgtgtttcc aggcaccagc
      781 cagttcgtta atcagtcctt ccgcaggatt gaagccgttg cctttatagc cctgctccgc
      841 ccaacgttcg aggatcaagc ccgcttcggt atgacaggag gcagaatcgg cgttgtattc
      901 gtagactttc aggccacgtt catccatgca gaaatccata cgaccagtga tcatatgatg
      961 acggcgacgc tgccaggaga gacgcaaacg tggccagagg attttcggaa tgtcaaacag
     1021 cgccagcagg ttgtcatctt tcagcacctt gtcggtggcg tgaagataca tcaggtgcag
     1081 ctcgttggtg gctttaatca gctcctgctc ggcactctcg gtaatggtgt agtaatgata
     1141 cggatcctga ttgatcacct ggccgttggc ctgtacatag gcgttttgca gcggatcttt
     1201 ttcatccagc catttaccgt caaactggcc tttgttttcc agacgcgccc cgctgatttt
     1261 cagcagttcg cctgcaattt ccggctgtgg caagctgtat tcggtatctt ccgtctggat
     1321 catccagccc agaatagtgg tgtcatcgaa ggtgtctttc agggtatagc cgccgttttc
     1381 gaccaccatc tccagctcgc gtgtccactg ttgcccttgc ggcaacgggg aatgaatcac
     1441 gttctgttcc gcaatacgga ctttgttgcc atgcaattgg gtaatgatgg cgacatggcc
     1501 agtgtcttta aattcaccgc ctttatccca gataagaagc gcccccgcta ccggtgcacg
     1561 cggcgaaccg ttaggaaatg cctgcaatgg caggatgttg tcattgacca cttcacgcag
     1621 gaagcgcagc gagaaaatct cccacgccat acccacgtca gtgaaaacca caccgtagtt
     1681 cagaaagaga aaacggcgag caaattcaac gcattgccat ttgtgaccca tatattcatc
     1741 gtcgatatag ctacggaata cggcgtcatc ttcgtattcc tgcggatcga gagaactgta
     1801 atctgaagag tagattgcta ccccacctgg ggcgtagccc aataatgtcc cgaacggggc
     1861 atcctggctg gtcgttcctt tgctcatcac tttaccttta acaatacaac ctaagcagtt
     1921 ggcgtaattg ttgtgctctg attacctgct cagcgacatt aaccggacag aggtcagact
     1981 aatcatacac ctcatcgcag cggctggcgc aaagggttaa aaaattcaca ttctgtacag
     2041 caagtgacct gctacactgc ttcaacacta ccactcagaa ggcaactcac tatgacagac
     2101 aatacttatc agcccgcgaa agtctggacg tgggataaat ccgctggcgg cgcgttcgcc
     2161 agtatcaatc gcccggtttc tggtccgacg catgaaaaaa cgctgccggt gggcaaacac
     2221 ccattgcaac tttattcgct gggaacgccg aacggtcaga aagtgacgat tatgctcgaa
     2281 gaactactcg cactgggcgt tactggtgca gagtacgacg cctggctgat tcgcattggc
     2341 gatggcgatc aattctccag cggctttgtc gaagtgaacc cgaattcgaa gatcccggca
     2401 ttgcgcgatc atacccataa tccgccgatc cgcgtgtttg agtcaggctc gattctgctt
     2461 tatctggcgg agaaatttgg ctacttcttg ccgcaggatc tggcaaagcg tactgaaacg
     2521 atgagctggc tgttctggtt acagggcgcg gcaccgttcc tcggcggtgg ttttggtcac
     2581 ttttaccatt acgcgccggt gaaaattgag tacgccatca accgctttac tatggaagcc
     2641 aagcgccttc tcgacgtgct ggataagcaa ctggcgcagc ataagtttgt tgcgggcgat
     2701 gagtacacca ttgcggatat agcgatttgg ccgtggtttg gcaatgtggt gttaggcggc
     2761 gtatatgatg ccgcagagtt tcttgatgcg ggcagttata agcatgtaca acgctgggcg
     2821 aaagaagtag gcgaacgtcc ggcggtgaag cgtgggcgta ttgttaaccg catcaacgga
     2881 ccgctgaacg agcagttgca tgaacgccat gacgccagtg atttcgagac aaatacggaa
     2941 gataagcgtc aggggtaatg gttagcgttc gtcgcagcaa gccatccaga ccaatgtagg
     3001 ccggataagg cgttcacgcc gcatccggca atcaggcaca atgcctgatg cgacgctgcc
     3061 gcgtcttatc aggcctacga aagcatccgg ctttaatcat cacgcgctgg taatgtcgta
     3121 atccatttgg cgcagtgcgt ctaatgtggc tttggcttcg tcttcatcga taatgctcat
     3181 ggcaaatccg acgtgcacca gtacccactg gcccagcaga tcggcagggt taccttcaca
     3241 aatcagggcg atattcacgt cgcgcttgat accacatact tcaacctgcg caagctggtg
     3301 aatatcttca ccgacagcca gcacttggcc tggaacgcca atacacattg ttaactccgg
     3361 ttattcaact tcaatacttt tgacgattag cgaatcgccg gtatcgacac gcaaccgctc
     3421 gccgtgacag atcggacact gcgcatcgtg ctgatgaatc tccaccacct ggctgcaatc
     3481 ccaacaccag gcctgggcgg gtttatagac gatatgtaaa tcgcaccctt gcgctaccgt
     3541 tccgtggcag acaatttcaa aactaaaacg gacggcgctc tcctcaacgc aggagagtgc
     3601 accaatttcc agccacacgg cggtgacgcg cttaacatcg tgctgctccg cctgccgttg
     3661 gataatttca acggcgctct ggcaaagcga caactcatgc attttcgcca ctcctgcggc
     3721 caaatagcag ggcacgacgc cctgcgtgtg gtacatccgg attggtgact ggcagcgaaa
     3781 ggatcattcg ggcgcagtca tccgtcagcc gttggccctc ttcaatcgac atgctgtgcg
     3841 aaagcggcga catcagcgag caggagaggt attgcgaaac accatccagt tcaccaacag
     3901 taaaggtcat agtgccatac ggcagttgca gaccaatttt ttcactcact ttgcgcagcg
     3961 gccagagttg atccgggccg gggaagataa ctgcactcag catccacggg gtgatcacac
     4021 accccgtcca ctgaccttcg aacagcgtaa agtcagaaac atacaccggc attgaaggat
     4081 gcagaaaaga gagatcatgc atcgaacgcc gggcaatttc ttcaaaagct acctgtactt
     4141 gcgccttcgg ggaggtctgg aaacctgcta tctcttcagt catgagtcgc ctcccgtggg
     4201 atagcttcca cgcctgattc gcgcagcgca gccagaacct gctcaagcgc aggttcaatc
     4261 atcgcttcga ccgtcggtgt caaaccgatg tgtggctcca gtgattccgg gatcacgccg
     4321 accagggtca gctttttcgg aaactcgccg gtgaagcgca gggccgacaa tacgtcggcc
     4381 aggccaagct gatgcggaga gattttgttg gtaaacaacg ccggaacttc ttcatcccgc
     4441 aggatcatca tcgttcccgg tgcgttcttt ttcgacacaa tggcatccgc aataatcaga
     4501 tgatcgcgat ttgccatgtc gccaagcagc tccattcccg ccgtgccgcc atcgaggatc
     4561 tcaacataat ccggcagaat gtatcgttgc tctaacgctt caacaatccg cacaccgatg
     4621 gcttcatcgg tcagcaaaat attgccgacc cctaagacta aaatacgcat tacagaacct
     4681 tcactgaaac cacttcgttg ccgtcggcat ccactacgtg taccgcacag gccatgcacg
     4741 ggtcgaagga gtgaatggta cgcaccactt ccagcggttt attcggatcg gcaaccggtg
     4801 tacccaccag cgactgctcg taaggaccga cgtcatcatt gaagttacgc gggccagagt
     4861 tccaggttga tggaacaact gcctggtagt tgctgatgat accgtctttg atcaccatcc
     4921 agtgagagag cataccacgc ggcgcttcga ggaagcctac gcctttgaac tcacccgttg
     4981 ccggaatgtt cggcttcaca aaggtggtgt gatcgccttt gccgatattg gtgatcagtg
     5041 cactgtattg attttgcagg atatcctgca attcacagca gtgaacagta cgaccaataa
     5101 tacggcccag cgtggagtgc agctgcgcca cttccagcgt gttgccagtc agtttctgat
     5161 aaatcgcaac gatttcattc agtttgttct gggtagactc gcgtcctgcc gccagtttca
     5221 ccagcatgtt agccagcgga cccacttcta ccgttttgcc gtagaaagtc ggtgatttca
     5281 cccaggaata tttaccgtcg tcagaccaac catcataagc cggaatggtg gtaccttccc
     5341 acggtgcctg cggcgcttcg tctttatacc aggagtgctt cgcgctttcc tgaatccctt
     5401 tgatcaggta ttcatcggaa tgagaagtga tcggacgata cgaggacaga tccgcattct
     5461 caatgtagcc gcccgggaac aggaagctgc catttttact gtcggtcggg aattccggca
     5521 cgctcaggta gttcaccgca cctttaccgc gcgtcagcca ttccgggtag aacgcagcga
     5581 taactgcagt atcaaccttg taaacctgct caacaaagtc gctcagcttg tcgatgaagg
     5641 acttgatgta catcaggcgc tcaaggttca gcacacccag accgtcgagg ttgatcgggt
     5701 tcgcgacacc acctaccgcc aggttctgaa tatgcggcgt tttaccgccc agcagcgcca
     5761 caacgcggtt agcgtcacgc tggcactcca gcgcctgcag gtagtgcgct accgcaatca
     5821 ggttcacttc cggcggcagt ttcattgccg ggtgacccca gtagccattg gcgaaaatac
     5881 ccaactgacc gctggcaacc agatctttga tcttgttctg aactttggtg aactcttccg
     5941 ggctgttcag atgccaggtc gaaacgcctt tcagcatttc ggaggctttg gctggatcag
     6001 cttgcagtgc agaagtgatg tccacccagt ccagcgccga aagctgatag aaatgaacaa
     6061 tatggtcatg cgtggtgtgc gcagccagaa tgatgttacg gatgtattgc gcgttaaccg
     6121 gaacgtcgat attcagcgca ctttctgccg cacgaacgga agacagcgcg tgagtggtag
     6181 tacatacgcc acagatacgt tgcacaatca tccatgcatc gcgcggatcg cggtttttca
     6241 cgatctcttc catgccgcgc cacatggtac cggaagccca tgctttcgaa acgacgccat
     6301 tttcgatttc gcaatcgatg cgtaaatgcc cttcaatacg ggttaccgga tcaatagtaa
     6361 ttctctggct catgctttgc tcgcctcatg acgattatga tcgttttgtt ttaaaggagg
     6421 aagtatcggc agtagacgaa tgagtacgat ataagcgcaa atctcaatag ccacaaaacc
     6481 aatagaaatc aacagttctt cccaggtcgg gaagtaggcg taaccgccac ccgggttgaa
     6541 tgccaccagc gaataggtca gacgccaggt tgcacaacct aacagtgcac tcagtgctga
     6601 caggaacagc atgcgggaat cattacgcaa cttcgccaca cgcagaacga ccagcgggaa
     6661 gagcatcagc aggacttcaa tccagaacat cacggagtag aagtcaccgg caaacgctaa
     6721 cgacagcttg tcgcgataga taagctcgcc aaagcgcagc acgatgaaaa tcgccagcaa
     6781 cacactgatg gtgttggtca gcttaacgaa cagactcttt tcatccggac cgttgccacg
     6841 cagacccgcc tgcaccaacg aaccttcaaa gatgacaatc gagaagccca tgatgaacgc
     6901 cgtcagcagc gagaacagcg gcaacatttc atagctttgc cacagcggat gcaccttgta
     6961 gcccgccgag atcatcagcg accccattga agactggtgc atagttggca gcagcgcacc
     7021 aagcgcgatg atgaagaaca tcaccttgtt cagacgtttg agtgacacct tccagcccag
     7081 acgttcaaac aatgccggag caaactccag tgccatcaca ccgatgtaga tggtcataca
     7141 gaccgccgtc tcgaacagta ccgagttcac gttgaagtga cccggaatgt agaagtacgg
     7201 caggttccag tagcgaccca cgtcgatagt gatcgacaag ccacccagtg aataaccaaa
     7261 cagactcgcc aacagcgccg gacgcaccag cggatggtat tgcccacggt taaagacgta
     7321 tactgcccac gccagcgccc agccgccaca ggcaaagccg gtaccgatca gcaggtcaaa
     7381 cgcgatccac acgccccacg ggaagccgcc gttcaggtcg gagacagagc ccagaccgaa
     7441 caccagacgc ttcacaatca ggagcataca gatgacgatt aacggtccaa aaatcatgac
     7501 cggtttactg atgattttgc cgcccagcgg ttgtggatca tgactcatga tcgtctcctc
     7561 cgtcgtgatg gtcgttttta gtgttgcgac gaaccagcac ggttaagccc gccagcacag
     7621 ccagtggtag catcatgcct ttatacaggg tgtgttggac atgttcggaa cgcgcaccag
     7681 tagaaagatc gtccagtttc ggcaggtcga gattttcata aggcacaccc gtcagtacca
     7741 gaacctgagt accgccgccc tctttctcgc cgtacagatg cggataatat ttcggcaccg
     7801 tatgcaggta agtgtcgcca gatttcagcg tctgacgcgg atagtggtat tcgctgcccg
     7861 gcttcagcgc cagacgtttt ttcgcctccg ccatcagctc ttcacgcgta ccaaaaatca
     7921 ccgcgcccgc cgggcacact tctacgcagc caggcagacc gcctttatcg agacgttcca
     7981 cacctttctg gttgcacaac tcgcacttat gcagcgcacc aaacgggttg ttgtagtcgt
     8041 acttcggcac gttgtatggg caggcgacca tgcagtaacg gcagccggtg cacacatctt
     8101 tgtcgtaatg gacaatgccg gttttcggat cttttttcag cgcagagacc gggcacacag
     8161 agacacagtt cggatcgacg cagtgcatac actgtttctt aatgtacgcg tagccattct
     8221 cctcctggtc tttgttgacc cctgtgccgc tggtccacac ctgaatgatg ttattggtat
     8281 acggcgacag tttgtcgttg ttcgaccagg tctgttcccc ttgcgggtta cgttcaggga
     8341 agttgatatc ctgacacttg gtgacgcaag cctggcagcc tacgcacaag gtcgagtcgt
     8401 acaacatccc cagcgatccc ggaattggcg ggcggttttc agcaaccgca tgactgacag
     8461 acggcagcgc gcccgtcagc aatgccccgc aggaggctgc tttaataaaa ttacgtctgt
     8521 tcacggttat tctccccgtg agtcagcgtt atctttcttt tgctgacgac ccagttcacg
     8581 caccgccatc acgctgacac cggcaaccag cccaaccaca ccgccgagca aaccaatagc
     8641 gcctgccgag acgttgccgc cttctttagc gttaacgtcc ggtttctgtg aacgcggagt
     8701 ctggttttcg acgttggcaa gctgatggat gcctttatgg aagccgatac cttcttcgtt
     8761 acagccatag cacgggtgac caatcgctac cggccacacg ccgccaacat cgcagaattg
     8821 cagcgttgag cagttaccgt aagtttctgg ccctttacag ccgaggtggt acaggcacca
     8881 gccttcgcgg tgaccttcat caccgaactc tttggcaaaa cgaccagcat cgaagtgcgg
     8941 gcgacgttca cagtgttcgt gaatcagacg accataggcg aaagtcggac ggtttttgtc
     9001 atccagtttc ggcggtttgc cgtaagtgat gatgtgcgca acggtcgcca ggaagttgtg
     9061 cgggttcggc gggcagccag gaatattgat aacggttttg cccggtagaa cttcttgcag
     9121 gctgactgcg ccagttgggt taactccagc tgcggcaaca ccgccccacg cagagcagga
     9181 accgatagcg ataatggctg ctgcgccttc cgccgctttg cggatgtgat ccacaatcgg
     9241 ctcaccggca accatgcaat aaataccgtt atcttttaat gggatggaac catccaccac
     9301 taacacatac tgccctttgt acttctcgag agcgttatgt ttgttctctt cgacctgatg
     9361 accgaaggcg gcggaaagca cttcgtgata ctccagagag atagtctcca gcacgaggtt
     9421 ttctaccgtt ggatgcgttg cacgaagcag agattccgta caaccggtgc actcctgcgc
     9481 gccaatccag ataactggcg gacgctgcgg gttagtaacc gattcggcca tctctgcagc
     9541 ggctttgcta cttaacccca tggtggcggc taatgctgca caaagcttca tgaaatcacg
     9601 acggttaatg ccgtgagaat ggatgagggt gttatctcca gtcatttata gttattccgt
     9661 tgcgaagacc tggcatatat tttgcctcaa tcgcaaaatc aataatgcga tcgatgcgcc
     9721 atttaccaca catttattat ggttatcgat atcatgatac tgcggcgggg cactaaaacg
     9781 aagggaagaa gcattagtgt aattaattaa agcaagataa tacgtatgtt tgatcaattt
     9841 tctaggcgat aaccggccat aaaaaagcgc aacgtagaac aggaattatt ctcacgaaag
     9901 aaatccatta ccatctcttt atccagtcca taacggcgcg tcagaatacc cgcttcccag
     9961 gcgctaagct ggcgatctcc ggtcttttcg tacatccggt gtgagtagcc taatacaaaa
    10021 ccgcgtttat aatcggcaca gaattttgtc acattaaccg cactgtcggc atgcgttgct
    10081 tttaatcccg ccattaagcc tttaccaaaa tggttattca tcgttcacct cattcgctat
    10141 atattgtcat atatagcgat attttaggca aagaacgaca tttgcgcacg ctattttttc
    10201 atgcttgttt aagacgtaat cctgtaagag ataaacccat caggagagat atttatcgaa
    10261 ccatttcagc gtcctttgcc aggcaagatc ggcggcagat ttgtcataac ggggcgtgga
    10321 atcattatgg aatccgtgat taacccccgg atatatatac gcctcataga ccttattatt
    10381 ggctttcaac gccgcctcgt aagcgggcca gccctcgttg attcggctat ccagttcagc
    10441 gtagtggagc agtaaaggcg cttcaatctt cgccacatcg gcagtgggtg cctgacgccc
    10501 ataaaacggc accgcgcagg ccagttccgg atacgcgact gctgccgcgt tcgatacgcc
    10561 cccgccatag caaaatccgc taatacccac tttgcctgtc gcttgcggat agcgttgcat
    10621 aaactcaatt gcggcaaaga aatcattcat cagtttggtt ggatcaacct gctgttgcag
    10681 ttcgcggcct ttatcatcgt taccaggata gccacctact gagcttaaac cgtctggcgc
    10741 cagggcgatg tacccttcct tcgccactcg ccgcgtcaca tcttcgatat acggattcag
    10801 ccctcgattc tcatgcacca ctaccacggc tggcgtttta ccgctcattt ttgcgggctt
    10861 caccagataa ccccgtacct cgccgtgacc atttggcgag ggatacgtga tgtactctgc
    10921 aacaatttcc gggtcggtaa actctacctg agtcgccagc gcataatttg gcttgagcaa
    10981 atcaaacagc gctaatgccg tcatcccgcc caccgcatac ttcgccgcaa ggttgaggaa
    11041 ctcacgtttc gagattttcc cgtgagcgta atagtcgtag taatccagca actcttgtgg
    11101 gaaagctttg gcagttaaac gcggcatcgt tgcactcctc aactggtgtt tttttaagca
    11161 aagcataagc gcgtgttttt gcccagtttt tcgtcactct gtgagccaga ctacgggata
    11221 cgcgctggcg aatcgctaaa ctagaaacat tgtttcgaaa ttgaatggtg gaaaggagag
    11281 gtcatggtct ggttagcgaa tcccgaacgt tacgggcgga tgcagtaccg ctattgcgga
    11341 aaaagtggtt tacgcctgcc cgcgttatcg ctcggtttat ggcacaattt cggtcacgtt
    11401 aacgcgctgg aatcacagcg tgcgatcctg cgtaaagcgt ttgatttagg cattacgcac
    11461 tttgatttag ccaacaatta cgggccgcct ccaggaagcg cagaagaaaa ctttggtcgc
    11521 ctgctgcgcg aggattttgc cgcttatcgc gatgaactga ttatctctac caaagccgga
    11581 tacgatatgt ggcccggccc atatggctct ggcggctcac gtaaatacct gctcgccagc
    11641 ctcgaccaaa gcctgaagcg tatggggctt gagtatgtcg atatctttta ctctcatcgc
    11701 gtcgatgaaa atacgccgat ggaagaaacc gcctctgcgc tggctcatgc ggtacaaagc
    11761 ggtaaggcgc tgtatgtcgg gatctcctct tactcgcctg agcggacgca aaaaatggtc
    11821 gagttgctgc gcgagtggaa aattccgctg ttaattcatc aaccttcgta caatttactg
    11881 aaccgctggg tggataaaag cggcctgctg gataccctgc aaaataacgg cgtgggctgc
    11941 attgccttta ctcctctggc tcagggattg ctgaccggaa aatatctcaa cggcattccg
    12001 caagattcac ggatgcatcg tgaagggaat aaagttcgtg gtctgacgcc gaaaatgctc
    12061 accgaagcca acctcaacag cctgcgctta ttgaatgaaa tggcacagca gcgtggacaa
    12121 tcaatggcgc aaatggcgtt aagctggttg ctgaaagatg agcgagtgac gtcggtattg
    12181 attggtgcca gccgcgcgga gcaacttgag gagaacgtgc aggcgctgaa taatctgaca
    12241 tttagcaccg aggagctggc gcagattgat cagcatatcg ccgatggaga gctgaatctg
    12301 tggcaggcgt cttccgataa atgacctgtt aataacgggc gcgagaaccg cgcccgaatc
    12361 agatcagtga ttatgacgag tcagtcggtc aagatagccc atcacaaatg cagagagcac
    12421 aaacgtcaga tggataatga cgtaccacat cagtttgtta tcagggacat ttttcgcatc
    12481 cataaagacg cgcagtaagt gaatggaaga aattgccaca atcgacgctg ctactttgtt
    12541 tttcagcgac gttgcgtcca ttttccccag ccagttcagc ttctctttgt tctcagagat
    12601 atcgagttgt gagacgaaat tctcgtaacc ggaaaacatc accatcacca gcaaaccgcc
    12661 aaccagcgtc atatccacca gcgacagcaa cacgaggatc aaatctgatt ccgccatcga
    12721 gaagatattc ggcagtacgt gaataatctc ctggaagaac ttcagcgcca gggcaactaa
    12781 cgccagcgaa aggccaaagt acacgggggc aagcagccag cgagaagcat acattgcatt
    12841 ttcaagaaaa cgttccatac attcctgtcg tgtagttaaa acgggcaaca gtatatcgca
    12901 attaagtaaa cagcacgcaa cagcaaatca acatatacgt taaacctttg tcgcataatt
    12961 ctgagggtta gggatcttcc tggttccctt atcaacgcat cgagagcgtc tacccttaaa
    13021 ggacgttgaa cctgaagggg gaaaaatgat gtctcattta aaagacccga ccacgcagta
    13081 ttacactggt gaatatccca aacagaaaca accgacgcca ggcatccagg cgaagatgac
    13141 accggtaccg gattgcggcg agaaaaccta tgttggtagc ggtcgcctga aagatcgtaa
    13201 agcactggtg acagggggcg attccggaat cggtcgcgct gccgccattg cttacgcgcg
    13261 tgaaggggct gacgtggcga tcagttatct tcccgtggaa gaagaagacg ctcaggatgt
    13321 gaaaaagatc attgaagaat gcggacgcaa agccgttctg ctgccaggcg atttaagcga
    13381 tgagaaattc gcacgttcgc tggttcacga agcacacaag gcgttaggcg ggctggatat
    13441 tatggcgctg gtagccggga aacaggttgc cattcctgat attgcagacc tcaccagcga
    13501 acagtttcaa aagacctttg ccattaacgt tttcgcgttg ttctggctaa cccaggaagc
    13561 gatccccctg ctaccgaaag gtgcaagtat catcaccact tcctcaatcc aggcatacca
    13621 gccaagcccg catttactgg actatgcggc tacgaaggcg gcaattctga actacagccg
    13681 tggcctggca aaacaggtcg cggagaaagg tattcgggtg aatattgtcg cgccaggccc
    13741 gatctggaca gcactgcaaa tttctggcgg acaaacgcag gataagatcc cgcagtttgg
    13801 tcagaaaacg ccgatgaagc gtgcggggca accggcggag ctggcccctg tatatgttta
    13861 cctggcaagt caggagtcga gctacgtcac cgcagaagtg cacggtgtct gcggcggcga
    13921 gcatttaggt taaaaaaatg cccggttgtg aaaagtaacc gggcatcatt tttatttcgc
    13981 tttcgcagtt tcttcgccga ccagacctat tttcaggtaa cccgcctgat gcagcgtatc
    14041 cattaccttc atcaacgtct cgtaatcgac ggttttatcc gctcggaaga agatggtggt
    14101 gtctttcttg ccttcggtta acgcattcaa cgccgtaatc attgtttcat cggtgaccgg
    14161 atcgttacca ataaacatcg agttgtctgc tttcaccgac agataaaccg gtttttccgg
    14221 gcgcggctgc ggcgtgctgg tagaagcagg caaattcacc ttcacatcta ccgtcgctaa
    14281 cggtgccgcc accataaaga taatcagcaa aaccaacatc acgtcgataa acggcgtcac
    14341 gttgatatca tgcatttcgc cgttatcgtc gaggttttcg ttaagatgca tcgccatcgg
    14401 acattatcct gcacgtaatt tttgtgcgac acgaaccgga tgcgcagcgg cgctggcttc
    14461 cagatccagg tcacggcttt gcaacaacaa tacctgcgct gcaacatcac ccagcatcgc
    14521 tttaaagccg ccaatctggc gtgcaaatac gttatagata acgaccgcag gaatcgctgc
    14581 cacgaggccg attgccgttg ctaacagagc ttctgcaata cccggcgcaa cgactgccag
    14641 gttagtggtc tgcgtttgcg cgataccaat aaagctgttc atgatgcccc agaccgtacc
    14701 aaacagacca acgaacggcg aaatcgcgcc gatggttgcc agatagccgt taccacggcc
    14761 catttgacga cctactgcgg cgacccgacg ctccagacgg aagctggtac gttctttaat
    14821 accttcgtta tcgtcgctgc cttctgacag ttccagctca ttctgcgctt cattgagcaa
    14881 atgcaggctc aagcttttgc taccaaaatc agcggcgata tcgttggcct ggtttaagga
    14941 acgcgcttca gccagcaatt gctgctcgcg cttaagacga cgcttctgat tgaagaactc
    15001 cacgctctta ctgaagaaga ttgcccaggt gactacggag gccaaaataa gcccaatcat
    15061 cacgcactta acgacaatat cggcgtgctg atacataccc cagacggaga ggtccgtctg
    15121 cattaaatta ttacccactg tgtatctcca ggacgcaagt cacaaaatct gtgcataata
    15181 atatcaaaac gacgtcgaat tgatagtcgt tctcattact atttgcatac tgccgtacct
    15241 ttgctttctt ttccttgcgt ttacgcagta aaaaagtcac cagcacgcca tttgcgaaaa
    15301 ttttctgctt tatgccaatt cttcaggatg cgcccgcgaa tattcatgct agtttagaca
    15361 tccagacgta taaaaacagg aatcccgaca tggcggacaa aaagcttgat actcaactgg
    15421 tgaatgcagg acgcagcaaa aaatacacgc tcggtgcggt aaatagcgtg attcagcgcg
    15481 cgtcttcgct ggtctttgac agtgtagaag ccaaaaaaca cgcgacacgt aatcgcgcca
    15541 atggcgagtt gttctatgga cggcgtggaa cgttaaccca tttctcctta caacaagcga
    15601 tgtgtgaact ggaaggtggc gcaggctgtg tgctatttcc ctgcggggcg gcagcggttg
    15661 ctaattccat tcttgctttt gtcgaacagg gcgatcatgt gttgatgacc aacaccgcct
    15721 atgaaccgag tcaggatttc tgtagcaaaa tcctcagcaa actgggcgta acgacatcgt
    15781 ggtttgatcc gctgattggt gccgatatcg ttaagcatct gcagccaaac actaaaatcg
    15841 tgtttctgga atcgccaggt tccatcacca tggaagtcca cgatgttccg gcgattgttg
    15901 ccgccgtacg cagtgtggtc ccggatgcca tcattatgat cgacaacacc tgggcagccg
    15961 gtgtgctgtt taaggcgctg gattttggca tcgatgtttc tattcaagcc gccaccaaat
    16021 atctggttgg gcattcagat gcgatgattg gcactgcggt gtgcaatgcc cgttgctggg
    16081 agcagctgcg agagaatgcc tatctgatgg gccagatggt cgatgccgat accgcctata
    16141 taaccagccg tggcctgcgc acattaggtg tgcgtttgcg ccaacatcat gaaagcagtc
    16201 tgaaagtggc tgaatggctg gcagaacatc cccaagtagc gcgagttaac caccctgctc
    16261 tgcctggcag taaaggccac gaattctgga aacgagactt tacaggcagc agcgggctat
    16321 tttcctttgt gcttaagaaa aaactcaatg atgaagagct ggcgaactat ctggataact
    16381 tcagtttatt cagcatggcc tactcgtggg gcgggtatga atcgttgatc ctggcaaatc
    16441 aaccagaaca tatcgccgcc attcgcccac aaggcgagat cgattttagc ggaaccttga
    16501 ttcgcctgca tattggtctg gaagatgtcg acgatctgat tgccgatctg gacgccggtt
    16561 ttgcgcgaat tgtataacat tgccactttt ggacaatttt gcagacattt tattgtgaaa
    16621 agtcttaaat tgttgcgtcc gggatcaagg cgtcccggac gattcaggag tacaataggc
    16681 agataaaggc ttaaacgctg ttccacagga aagtccatgg ctgttattca agatatcatc
    16741 gctgcgctct ggcaacacga ctttgccgcg ctggcggacc ctcatattgt tagcgttgtt
    16801 tactttgtta tgtttgccac gctgttttta gaaaacggct tgctgcccgc ctcatttttg
    16861 ccaggcgaca gcttgttgat attggctggc gcattgattg cccagggggt tatggatttt
    16921 ctgcctacga ttgcgattct gaccgccgca gcaagtctgg gctgctggct aagttatatt
    16981 caggggcgct ggttagggaa taccaaaacg gtgaaaggct ggctggcaca gcttcctgct
    17041 aaatatcacc agcgcgccac ctgcatgttt gaccgccacg gtctgctggc gctgctggct
    17101 ggacgttttc ttgcatttgt ccgtacgctg ctgccaacca tggcgggaat ttccggtctg
    17161 ccaaaccgcc gcttccagtt tttcaactgg ttaagcggat tgctgtgggt cagcgtggta
    17221 accagtttcg gctatgcctt aagtatgatt ccgttcgtta aacgccatga agatcaggta
    17281 atgacgttcc tgatgatcct gccaattgcc ttgttaaccg ctggcttgtt gggcacgctg
    17341 tttgtggtga ttaaaaaaaa atactgtaac gcctgacgat tttccccgtt cccggttgct
    17401 gtaccgggaa cgtatttaat tcccctgcat cgcccgcatt cttgccgcat cttcccccgg
    17461 cgtcacaccg aagtaacgtt taaactcacg gctaaattgc gatgcgcttt catagccgac
    17521 gcgcatcgcc gccgcgctgg ccttcatgcc gtcgtggatg atcatcatcc gcgccttatg
    17581 cagacggtaa ttcttcaaat actgcaacgg cgaggtgctg gtgacagact taaaattatg
    17641 gtggaacgcc gatacgctca tgttggcttc tgccgccagt tgctcgacgc tcaggttttc
    17701 ggtgtattta ttctcaatcc gtttcagcac gcgactaatc aggctgaagt gagtctggcg
    17761 gctgaccagt gccagtaacg cgccgccgca aggtccggtc agcacgtagt acagaatttc
    17821 gcggatgatc tgtttgccga gaatacgcgc atccagcggt cgctccatca catccagtaa
    17881 ccgctccgcc gcgcataaaa tctcttctga taacgtggca gagttaatcc cgctggctgc
    17941 catcgacggc tggaaatgct catcttcgcc aatgtccatc aacagttcct gtaactgcaa
    18001 aatatcgaca ttgagacgca accctgccag cggcacctct gacgtggcat aggtttcgca
    18061 ctcaaacggc aacggcaccg tcagcaggag atattcatta gcgtcataac gaaacacgcg
    18121 ttcattgata taaccgattt tatgtccgga aaagagaatt atgatgccag gctcgtacat
    18181 caccggtgtg cgtgcgaaag gcgtctcgcc atacaacaaa cgcacatcgg gcaacagctc
    18241 tgacaaacta ttttctttat ttttcagttt attaacttta tccgccagca agcggcaaat
    18301 ctcttcacgt ttcatatcgc gtaatttctt aggaataatg cggcaatttg attgtgcgca
    18361 attttgtagc atttctccag cactctggag aaataggcaa gacattggca gaaatgagca
    18421 ttgagaggta cggcgctggc gaccacaatg aaaaacatca ggcagaccga tctctgccct
    18481 catattgacc cagcaaaggg agcaagtaat gaacaacttt aatctgcaca ccccaacccg
    18541 cattctgttt ggtaaaggcg caatcgctgg tttacgcgaa caaattcctc acgatgctcg
    18601 cgtattgatt acctacggcg gcggcagcgt gaaaaaaacc ggcgttctcg atcaagttct
    18661 ggatgccctg aaaggcatgg acgtgctgga atttggcggt attgaaccaa acccggctta
    18721 tgaaacgctg atgaacgccg tgaaactggt tcgcgaacaa aaagtgactt ttctgctggc
    18781 ggttggcggc ggttctgtac tggacggcac caaatttatc gccgcagcgg ctaactatcc
    18841 ggaaaatatc gatccgtggc acattctgca aacgggcggc aaagagatta aaagcgccat
    18901 cccgatgggc tgtgtgctga cgctgccagc aaccggttca gaatccaacg caggcgcggt
    18961 gatctcccgt aaaaccacag gcgacaagca ggcgttccat tcagcccatg ttcagcccgt
    19021 atttgccgtg ctcgatccgg tttataccta caccctgccg ccgcgtcagg tggctaacgg
    19081 cgtagtggac gcctttgtac acaccgtgga acagtatgtt accaaaccgg ttgatgccaa
    19141 aattcaggac cgtttcgcag aaggcatttt gctgacgctg atcgaagatg gtccgaaagc
    19201 cctgaaagag ccagaaaact acgatgtgcg cgccaacgtc atgtgggcgg cgactcaggc
    19261 gctgaacggt ttgatcggcg ctggcgtacc gcaggactgg gcaacgcata tgctgggcca
    19321 cgaactgact gcgatgcacg gtctggatca cgcgcaaaca ctggctatcg tcctgcctgc
    19381 actgtggaat gaaaaacgcg ataccaagcg cgctaagctg ctgcaatatg ctgaacgcgt
    19441 ctggaatatc actgaaggtt cagacgatga gcgtattgac gccgcgattg ccgcaacccg
    19501 caatttcttt gagcaattag gcgtgccgac ccacctctcc gactacggtc tggacggcag
    19561 ctccatcccg gctttgctga aaaaactgga agagcacggc atgacccaac tgggcgaaaa
    19621 tcatgacatt acgctggatg tcagccgccg tatatacgaa gccgcccgct aagcttttta
    19681 cgcctcaaac tttcgttttc gggcatttcg tccagactta agttcacaac acctcaccgg
    19741 agcctgctcc ggtgagttca tataaaggag gaacgtatgg ctaatccaac cgttattaag
    19801 ctacaggatg gcaatgtcat gccccagctg ggactgggcg tctggcaagc aagtaatgag
    19861 gaagtaatca ccgccattca aaaagcgtta gaagtgggtt atcgctcgat tgataccgcc
    19921 gcggcctaca aaaacgaaga aggtgtcggc aaagccctga aaaatgcctc agtcaacaga
    19981 gaagaactgt tcatcaccac taagctgtgg aacgacgacc acaagcgccc ccgcgaagcc
    20041 ctgctcgaca gcctgaaaaa actccagctt gattatatcg acctctattt aatgcactgg
    20101 cccgttcccg ctatcgacca ttatgtcgaa gcatggaaag gcatgatcga attgcaaaaa
    20161 gagggattaa tcaaaagcat cggcgtgtgc aacttccaga tccatcacct gcaacgcctg
    20221 attgatgaaa ctggcgtgac gcctgtgata aaccagatcg aacttcaccc gctgatgcaa
    20281 caacgccagc tacatgcctg gaacgcgaca cacaaaatcc agaccgaatc ctggagccca
    20341 ttagcgcaag gagggaaagg cgttttcgat cagaaagtca ttcgcgatct ggcagataaa
    20401 tacggcaaaa cccctgcgca gattgttatc cgctggcatc tggatagcgg cctggtggtg
    20461 atcccaaaat cggtcacacc ttcacgcatt gtcgaaaact ttgatgtctg ggatttccgt
    20521 ctcgacaaag acgaacttgg cgaaattgca aaactcgatc agggcaagcg tctcggtccc
    20581 gatcctgacc agttcggcgg ctaacgtgca aattctcccg gtggcggtaa tgttccgcta
    20641 ccggactttt cagaaatcat ttattcccct cgcgtcccgc ccgttgctac tcttccttgt
    20701 tcaggaatgc caaatataag gacatcatca tgcagagccg gaagctctta aaagaacaac
    20761 tcatctatat ccgggataaa cgcaacggag aggtgaaaaa cagatgaaaa taatacttct
    20821 gtttttagca gccctggcaa gttttaccgt acacgcacag cccccctcac agaccgtaga
    20881 acaaacagtc cggcatattt atcagaacta taaatcagat gccactgccc cttattttgg
    20941 tgaaaccgga gagcgggcga taacatctgc gcgtattcaa caggcgctta ccctgaacga
    21001 caatcttacg ctgccgggca atattggctg gctggattat gatccggttt gtgattgtca
    21061 ggattttggc gatctggtgt tagaaagcgt agcgataacc caaactgacg ccgatcatgc
    21121 cgatgccatt gtgcgctttc gtatctttca agacgataaa gaaaagacct cgcagacact
    21181 gaaaatggtg gcggaaaatg gtcgttgggt cattgacgat attgtcagca atcatggcag
    21241 cgtcttacaa gcggttaata gcgagaatga aaaaacactg gccgctatag cttcgttgca
    21301 aaaagaacag ccggaagcct ttgttgccga actctttgaa catattgctg attatagctg
    21361 gccgtggacg tgggtggttt ccgactctta ccgccaggcg gtcaatgcct tctataaaac
    21421 caccttcaag acggccaaca atcccgatga agatatgcaa atagaacggc aatttattta
    21481 cgacaatccg atctgttttg gcgaagagtc gttattttcc cgcgttgatg aaattcgagt
    21541 cctggggaaa accaccgatt ccgcccgcat tcatgttcgc tttacgctga ccaatggcaa
    21601 caacgaagag caagaactga ttttacagcg gcgcgaaggt aagtgggaaa tcgctgattt
    21661 tatccgcccg aacagcggca gcctacttaa gcagattgag gcaaaaaccg ccgccagatt
    21721 aaaacaatga gctgaattaa ataacaatta gccggaacaa taaataaaag ggaacactat
    21781 atgaaaacga ttttcaccgt gggagctgtt gttctggcaa cctgcttgct cagtggctgc
    21841 gtcaatgagc aaaaggtcaa tcagctggcg agcaatgtgc aaacattaaa tgccaaaatc
    21901 gcccggcttg agcaggatat gaaagcatta cgcccacaaa tctatgctgc caaatccgaa
    21961 gctaacagag ccaatacgcg tcttgatgca caggactatt tcgactgcct gcgctgcttg
    22021 cgtatgtacg cagaatgata aaaaaatccc cggcagcatt ctgtttccgg ggatattttt
    22081 taacgtccaa ccgccgcttt agggcgtttc ttcgcaccag cattctgcag gcgagattgc
    22141 gtagacgacg ctttttttgc cgtagcaggc gtctgacgct gggtcgccat cggcgtatgt
    22201 ttagtcaacg ccggacgggt attgcggttc tgacggcgag cttcacgcat ctcttcaatg
    22261 gttggcgcag gcactaagca atcgcgacgg ctgccaatca gatgcttttt gcccatcgct
    22321 tccagcgcct ggcggattaa cggccagttt gccggatcgt ggtaacgcaa caacgcttta
    22381 tgcaaacgac gctgcttgtc gcccttcggt acgaagacgt cttcactctt ataaccaatc
    22441 ttcgccagcg ggtttttgcc ggtgtaatac atggtggtcg agttagccag cggcgacgga
    22501 tagaagttct gcacctggtc gaggcggaag cgatgctttt tcagccatag agccagattc
    22561 accatatctt catcacgcgt acccgggtgc gcggagatga aatacgggat cagatactgt
    22621 tctttacctg cctgtttcga gtaagtatcg aacagctctt taaagcggtc atagctgccc
    22681 atgcccggct tcatcatctt cgataacggc ccttcttcgg tatgttccgg ggcaatcttc
    22741 agataaccgc caacgtgatg ggtcgccagc tctttgatat agcgcggatc ttctacggcg
    22801 atgtcataac gcacaccaga ggcaatcagg atctttttaa tgcctttcag atcgcgcgca
    22861 cggcgataga ggttgatcgt cggttcgtgg ttagtgtcca tatgcggaca aatatccgga
    22921 taaacgcacg acaaacgacg gcaggtttgc tcagcgcgtg gcgatttgca acgcaacata
    22981 tacatgttgg cagtaggccc accgagatcg gaaatcacgc ccgtaaaacc tggaacagtg
    23041 tcgcggatcg cttcgatctc attaataatc gaatcttcgg aacggctctg aataatgcgc
    23101 ccttcgtgct cggtaataga acagaaagag cagccgccaa agcagccacg cataatgttg
    23161 accgagaaac gaatcatttc gtaagccgga attcgggcat taccgtaagc cggatgcggc
    23221 acgcgcttgt acggcagcgc aaagacgcta tccatctctt cggtagaaag cggaatagca
    23281 ggcgggttga tccatacata gcggtcgcca tgtttttgca tcaatgcgcg ggcgcagcct
    23341 gggttagttt cgtggtgcag aatgcgcgaa gcatgggcgt acagcacttt atcgcccttc
    23401 actttctcga aagaaggcag caacacgtag gttttttccc acggtttcgg gcgtggtggc
    23461 tgcacggtta cagctttagc ttcctgcttt ttcggagcta ccggtttgtt atccgcgcac
    23521 ggcaaatctt cgccatacgg atgcgggatt gggtcgattt tccctggggt atcaagacgg
    23581 gtggaatcca caccgctcca gcctggcaac gcctctttta cgataatcgc ggtattacgc
    23641 acatcgcgga tttcactaat cggctcgccc atcgccagac ggtgcgccac ttccaccagc
    23701 ggacgctcac cgttaccaaa catcagcatg tcggctttcg aatccaccag cacggaacgg
    23761 cgcacggtat ccgaccagta atcatagtgc gcggtacggc gcaggctggc ctcaataccg
    23821 ccgaggatca ccggcacatc tttccacgcc tctttacaac gttgggtata aaccagtgtg
    23881 gcgcgatccg gacgcttacc cgcgacgtta tccggtgtgt aggcatcgtc atgacgtaaa
    23941 cggcgatcgg tggtatagcg gttgatcatc gagtccatgt tgccagcagt aacaccgaaa
    24001 aacagattcg gtttacccag acgcataaag tcgtctttac tgctccagtc tggctgggcg
    24061 atgatcccga cgcgaaagcc ctgcgcttcc agcatacgac cgcaaatcgc catcccgaag
    24121 cttgggtgat cgacatacgc gtcgccagta accaaaatga tgtcgcagct atcccagcca
    24181 agttgatcca tctcttcacg tgacatcggc aaaaacggtg ccggtccaaa acaggcggcc
    24241 cagtactgcg gccaggagaa caggtcgcga tccggttgga tcagggagat agagctcatt
    24301 ttgcttccag aaatggtaaa aaaataatca aaggccgggg attatacgct gtatcacgcg
    24361 cggaaatgaa gtgatgttga tgaacggtgg cgaaataccc tggtgaggtt tttcactgcc
    24421 cgacttgggc aagcacattt aaggctggcg gtatcgtggc aactgccgct actatttatg
    24481 ccgatcagca acgttcaaat catattgcac gacgatcggc tatccgagga cacccacagt
    24541 aaggcaaggt atggaacagg ttattacaaa gcgccgctat tacgatattg gtctacagat
    24601 tgaagagctg ctgtattcag gcgtatttaa ggccggagaa cgcttacctt cagaacgtga
    24661 actgagcgag cggtttaata ccagccgaac taccattcgt gaagccatca ttatgctgga
    24721 actgaaaggc gtattgaatg tgaagcaagg gtcagggatt ttctttgtcg actccaccga
    24781 taagctcaac cagaaatcat taatgcccta ttcagaaatt ggcccgtttg aactgttaca
    24841 ggcacgtcag gtaattgaga gcaatattac cggttttgct gccagccaga tcagctttaa
    24901 cgagttacaa gagttgaaga aaatcattgg attacaggaa aaagccatcg ctgcggaaag
    24961 tgataaattt gaagacctgg atcaccgatt tcatagcatt attgccgaag cgacgcaaaa
    25021 tcgggtactc atcaaacaag ccgctgaatt atggcgggca gtgcgcacag aaaacccccg
    25081 ctggaagaaa cttaactaca agtacctgca tgaaaagcat ttgcgtctgc aatggctgga
    25141 agatcaccgg gctattttcc tggctctgca acagaaagat tcagagctcg ccagggaagc
    25201 gtcctggaga catctggaaa acagcaaaaa tgaactgatc aaaatcttca aacaagacgc
    25261 ttccatcagc gattttgatg actttttctt cgctcgttaa ccttcattag tccagagctg
    25321 gcgggaaaaa taacgccggc tctgcctttc gccctccact ggtacacgct ctctttttaa
    25381 ttggtctaat aaatcgacta tcatcagata tcttagatta gcggcaatta tcctaaaaac
    25441 tgacctctaa aaaactttcc tttca
//
LOCUS       DADSYM010000066        25024 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2415
            SAMN08039579-rid11723173.denovo.066, whole genome shotgun sequence.
ACCESSION   DADSYM010000066 DADSYM010000000
VERSION     DADSYM010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039579
            Sequence Read Archive: SRR6362533
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 25024)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25024)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980162.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 03:39:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,484
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 4,347
            CDSs (with protein)               :: 4,347
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 43 of 85
            Pseudo Genes (incomplete)         :: 37 of 85
            Pseudo Genes (internal stop)      :: 19 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25024
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039579-rid11723173.denovo.066"
                     /strain="LGE2415"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8302 N 95.9628 W"
                     /collection_date="2015-08-24"
     gene            complement(184..903)
                     /gene="dcuR"
                     /locus_tag="J1Y83_002270"
     CDS             complement(184..903)
                     /gene="dcuR"
                     /locus_tag="J1Y83_002270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator DcuR"
                     /protein_id="HBA7630417.1"
                     /translation="MINVLIIDDDAMVAELNRRYVAQIPGFQCCGTASTLEKAKEIIF
                     NSDTPIDLILLDIYMQKENGLDLLPVLHNARCKSDVIVISSAADAVTIKDSLHYGVVD
                     YLIKPFQASRFEEALTGWRQKKMALEKHQYYDQAELDQLIHGSSSNEQDPRRLPKGLT
                     PQTLRTLCQWIDAHQDYEFSTDELANEVNISRVSCRKYLIWLVNCHILFTSIHYGVTG
                     RPVYRYRIQAEHYSLLKQYCQ"
     gene            complement(900..2531)
                     /gene="dcuS"
                     /locus_tag="J1Y83_002271"
     CDS             complement(900..2531)
                     /gene="dcuS"
                     /locus_tag="J1Y83_002271"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     DcuS"
                     /protein_id="HBA7630418.1"
                     /translation="MRHSLPYRMLRKRPMKLSTTVILMVSAVLFSVLLVVHLIYFSQI
                     SDMTRDGLANKALAVARTLADSPEIRQGLQKKPQESGIQAIAEAVRKRNDLLFIVVTD
                     MHSLRYSHPEAQRIGQPFKGDDILKALNGEENVAINRGFLAQALRVFTPIYDENHKQI
                     GVVAIGLELSRVTQQINDSRWSIIWSVLFGMLVGLIGTCILVNVLKKILFGLEPYEIS
                     TLFEQRQAMLQSIKEGVVAVDDRGEVTLINDAAQELLNYRKSQDDEKLSTLSHSWSQV
                     VDVSEVLRDGTPRRDEEITIKDRLLLINTVPVRSNGVIIGAISTFRDKTEVRKLMQRL
                     DGLVNYADALRERSHEFMNKLHVILGLLHLKSYKQLEDYILKTANNYQEEIGSLLGKI
                     KSPVIAGFLISKINRATDLGHTLILNSESQLPDSGSEDQVATLITTLGNLIENALEAL
                     GPEPGGEISVTLHYRHGWLHCEVNDDGPGIAPDKIDHIFDKGVSTKGSERGVGLALVK
                     QQVENLGGSIAVESEPGIFTQFFVQIPWDGERSNR"
     gene            2712..2942
                     /gene="yjdI"
                     /locus_tag="J1Y83_002272"
     CDS             2712..2942
                     /gene="yjdI"
                     /locus_tag="J1Y83_002272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S mono-cluster protein YjdI"
                     /protein_id="HBA7630419.1"
                     /translation="MDRALLDGGYRCYTGEKIDVYFNTAICQHSGNCVRGNGKLFNLK
                     RKPWIMPDEVDVATVVKVIDTCPSGALKYRHK"
     gene            2954..3226
                     /locus_tag="J1Y83_002273"
     CDS             2954..3226
                     /locus_tag="J1Y83_002273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HBA7630420.1"
                     /translation="MEIREGHNKFYINDEQGKQIAEIVFVPTGENLAIIEHTDVDESL
                     KGQGIGKQLVAKVVEKMRREKRKIIPLCPFAKHEFDKTREYDDIRS"
     gene            3455..3751
                     /gene="ghoS"
                     /locus_tag="J1Y83_002274"
     CDS             3455..3751
                     /gene="ghoS"
                     /locus_tag="J1Y83_002274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type V toxin-antitoxin system endoribonuclease
                     antitoxin GhoS"
                     /protein_id="HBA7630421.1"
                     /translation="MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEY
                     GIPRQLNENTFAITTSLAASEIEDLIRLKCLDLPDIDFDLSIMTVDDYFRQFYK"
     gene            3779..3952
                     /gene="ghoT"
                     /locus_tag="J1Y83_002275"
     CDS             3779..3952
                     /gene="ghoT"
                     /locus_tag="J1Y83_002275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type V toxin-antitoxin system toxin GhoT"
                     /protein_id="HBA7630422.1"
                     /translation="MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITW
                     PMSLPVALLFSLF"
     gene            complement(4071..5588)
                     /gene="lysS"
                     /locus_tag="J1Y83_002276"
     CDS             complement(4071..5588)
                     /gene="lysS"
                     /locus_tag="J1Y83_002276"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HBA7630423.1"
                     /translation="MSERETRGANEAIDFNDELRNRREKLAALRQQGVAFPNDFRRDH
                     TSDQLHEEFDAKDNQELESLNIEVSVAGRMMTRRIMGKASFVTLQDVGGRIQLYVARD
                     SLPEGVYNDQFKKWDLGDIIGARGTLFKTQTGELSIHCTELRLLTKALRPLPDKFHGL
                     QDQEVRYRQRYLDLIANDKSRQTFVVRSKILAAIRQFMVARGFMEVETPMMQVIPGGA
                     SARPFITHHNALDLDMYLRIAPELYLKRLVVGGFERVFEINRNFRNEGISVRHNPEFT
                     MMELYMAYADYHDLIELTESLFRTLAQEVLGTTKVTYGEHVFDFGKPFEKLTMREAIK
                     KYRPETDMADLDNFDAAKALAESIGITVEKSWGLGRIVTEIFDEVAEAHLIQPTFITE
                     YPAEVSPLARRNDVNPEITDRFEFFIGGREIGNGFSELNDAEDQAERFQEQVNAKAAG
                     DDEAMFYDEDYVTALEYGLPPTAGLGIGIDRMIMLFTNSHTIRDVILFPAMRPQK"
     gene            complement(5825..7282)
                     /gene="dtpC"
                     /locus_tag="J1Y83_002277"
     CDS             complement(5825..7282)
                     /gene="dtpC"
                     /locus_tag="J1Y83_002277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpC"
                     /protein_id="HBA7630424.1"
                     /translation="MKTPSQPRAIYYIVAIQIWEYFSFYGMRALLILYLTHQLGFDDN
                     HAISLFSAYASLVYVTPILGGWLADRLLGNRTAVIAGALLMTLGHVVLGIDTNTTFSL
                     YLALAIIICGYGLFKSNISCLLGELYDENDHRRDGGFSLLYAAGNIGSIAAPIACGLA
                     AQWYGWHVGFALAGGGMFIGLLIFLSGHRHFQSTRSMDKKALTSVKFALPVWSWLVVM
                     LCLAPVFFTLLLENDWSGYLLAIVCLIAAQIIARMMIKFPEHRRALWQIVLLMFVGTL
                     FWVLAQQGGSTISLFIDRFVNRQAFNIEVPTALFQSVNAIAVMLAGVVLAWLASPESR
                     GNSALRVWLKFAFGLLLMACGFMLLAFDARHAAAGGQASMGVMISGLALMGFAELFID
                     PVAIAQITRLKMSGVLTGIYMLATGAVANWLAGVVAQQTTESQISGMAIAAYQRFFSQ
                     MGEWTLACVAIIVVLAFATRFLFSTPTNMIQESND"
     gene            complement(7341..9488)
                     /gene="cadA"
                     /locus_tag="J1Y83_002278"
     CDS             complement(7341..9488)
                     /gene="cadA"
                     /locus_tag="J1Y83_002278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine decarboxylase CadA"
                     /protein_id="HBA7630425.1"
                     /translation="MNVIAILNHMGVYFKEEPIRELHRALERLNFQIVYPNDRDDLLK
                     LIENNARLCGVIFDWDKYNLELCEEISKMNENLPLYAFANTYSTLDVSLNDLRLQISF
                     FEYALGAADDIANKIKQTTDEYINTILPPLTKALFKYVREGKYTFCTPGHMGGTAFQK
                     SPVGSLFYDFFGPNTMKSDISISVSELGSLLDHSGPHKEAEQYIARVFNADRSYMVTN
                     GTSTANKIVGMYSAPAGSTILIDRNCHKSLTHLMMMSDVTPIYFRPTRNAYGILGGIP
                     QSEFQHATIAKRVKETPNATWPVHAVITNSTYDGLLYNTDFIKKTLDVKSIHFDSAWV
                     PYTNFSPIYEGKCGMSGGRVEGKVIYETQSTHKLLAAFSQASMIHVKGDVNEETFNEA
                     YMMHTTTSPHYGIVASTETAAAMMKGNAGKRLINGSIERAIKFRKEIKRLRTESDGWF
                     FDVWQPDHIDTTECWPLRSDSTWHGFKNIDNEHMYLDPIKVTLLTPGMEKDGTMSDFG
                     IPASIVAKYLDEHGIIVEKTGPYNLLFLFSIGIDKTKALSLLRALTDFKRAFDLNLRV
                     KNMLPSLYREDPEFYENMRIQELAQNIHKLIVHHNLPDLMYRAFEVLPTMVMTPYAAF
                     QKELHGMTEEVYLDEMVGRINANMILPYPPGVPLVMPGEMITEESRPVLEFLQMLCEI
                     GAHYPGFETDIHGAYRQADGRYTVKVLKEESKK"
     gene            complement(9568..10902)
                     /gene="cadB"
                     /locus_tag="J1Y83_002279"
     CDS             complement(9568..10902)
                     /gene="cadB"
                     /locus_tag="J1Y83_002279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadaverine/lysine antiporter"
                     /protein_id="HBA7630426.1"
                     /translation="MSSAKKIGLFACTGVVAGNMMGSGIALLPANLASIGGIAIWGWI
                     ISIIGAMSLAYVYARLATKNPQQGGPIAYAGEISPAFGFQTGVLYYHANWIGNLAIGI
                     TAVSYLSTFFPVLNDPVPAGIACIAIVWVFTFVNMLGGTWVSRLTTIGLVLVLIPVVM
                     TAIVGWHWFDAATYAANWNTADTTDGHAIIKSILLCLWAFVGVESAAVSTGMVKNPKR
                     TVPLATMLGTGLAGIVYIAATQVLSGMYPSSVMAASGAPFAISASTILGNWAAPLVSA
                     FTAFACLTSLGSWMMLVGQAGVRAANDGNFPKVYGEVDSNGIPKKGLLLAAVKMTALM
                     ILITLMNSAGGKASDLFGELTGIAVLLTMLPYFYSCVDLIRFEGVNIRNFVSLICSVL
                     GCVFCFIALMGASSFELAGTFIVSLIILMFYARKMHERQSHSMDNHTASNAH"
     gene            complement(11268..12806)
                     /gene="cadC"
                     /locus_tag="J1Y83_002280"
     CDS             complement(11268..12806)
                     /gene="cadC"
                     /locus_tag="J1Y83_002280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator CadC"
                     /protein_id="HBA7630427.1"
                     /translation="MQQPVVRVGEWLVTPSINQISRNGRQLTLEPRLIDLLVFFAQHS
                     GEVLSRDELIDNVWKRSIVTNHVVTQSISELRKSLKDNDEDSPVYIATVPKRGYKLMV
                     PVIWYSEEEGEELMLSSPPPIPEAVPATDSPSHSLNIQNTTTPPEQSPVKSKRFTTFW
                     VWFFFLLSLGICVALVAFSSLETRLPMSKSRILLNPRDIDINMVNKSCNSWSSPYQLS
                     YAIGVGDLVATSLNTFSTFMVHDKINYNIDEPSSSGKTLSIAFVNQRQYRAQQCFMSV
                     KLVDNADSSTMLDKRYVITNGNQLAIQNDLLQSLSKALNQPWPQRMQEMLQQILPHRG
                     ALLTNFYQAHDYLLHGDDKSLDRASELLGEIVQSSPEFTYARAEKALVDIVRHSQHPL
                     DEKQLAALNTEIDNIVTLPELNNLSIIYQIKAVSALVKGKTDESYQAINTGIDLEMSW
                     LNYVLLGKVYEMKGMNREAADAYLTAFNLRPGANTLYWIENGIFQTSVPYVVPYLDKF
                     LASE"
     gene            complement(13423..13498)
                     /locus_tag="J1Y83_002281"
     tRNA            complement(13423..13498)
                     /locus_tag="J1Y83_002281"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(13463..13465),aa:Phe,seq:gaa)
     gene            complement(13605..14180)
                     /locus_tag="J1Y83_002282"
     CDS             complement(13605..14180)
                     /locus_tag="J1Y83_002282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HBA7630428.1"
                     /translation="MQREDVLGEALKLLELQGIANTTLEMVAERVDYPLDELRRFWPD
                     KEAILYDALRYLSQQIDVWRRQLMLDETQTAEQKLLARYQALSECVKNNRYPGCLFIA
                     ACTFYPDPGHPIHQLADQQKSAAYDFTHELLTTLEVDDPAMVAKQMELVLEGCLSRML
                     VNRSQADVDTAHRLAEDILRFARCRQGGALT"
     gene            complement(14217..15914)
                     /locus_tag="J1Y83_002283"
     CDS             complement(14217..15914)
                     /locus_tag="J1Y83_002283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-disulfide reductase DsbD"
                     /protein_id="HBA7630429.1"
                     /translation="MAQRIFTLILLLCSTSVFAGLFDAPGRSQFVPADQAFAFDFQQN
                     QHDLNLTWQIKDGYYLYRKQIRITPEHAKIADVQLPQGVWHEDEFYGKSEIYRDRLTL
                     PVTINQASAGATLTVTYQGCADAGFCYPPETKTVPLSEVVANNEASQPVSVPQQEQPT
                     AQLPFSALWALLIGIGIAFTPCVLPMYPLISGIVLGGKQRLSTARALLLTFIYVQGMA
                     LTYTALGLVVAAAGLQFQAALQHPYVLIGLAIVFTLLAMSMFGLFTLQLPSSLQTRLT
                     LMSNRQQGGSPGGVFIMGAIAGLICSPCTTAPLSAILLYIAQSGNMWLGGGTLYLYAL
                     GMGLPLMLITVFGNRLLPKSGPWMEQVKTAFGFVILALPVFLLERVIGDIWGLRLWSA
                     LGVAFFGWAFITSLQAKRGWMRVVQIILLAAALVSVRPLQDWAFGATHTAQTQTHLNF
                     TQIKTVDELNQTLVEAKGKPVMLDLYADWCVACKEFEKYTFSDPQVQKALADTVLLQA
                     NVTANDAQDVALLKHLNVLGLPTILFFDGQGQEHPQARVTGFMDAETFSAHLRDRQP"
     gene            complement(15890..16228)
                     /gene="cutA"
                     /locus_tag="J1Y83_002284"
     CDS             complement(15890..16228)
                     /gene="cutA"
                     /locus_tag="J1Y83_002284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710002.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent cation tolerance protein CutA"
                     /protein_id="HBA7630430.1"
                     /translation="MLDEKSSNTTSVVVLCTAPDEATAQDLAAKVLAEKLAACATLIP
                     GATSLYYWEGKLEQEYEVQMILKTTVSHQQALLECLKSHHPYQTPELLVLPVTHGDTD
                     YLSWLNASLR"
     gene            complement(16344..17645)
                     /locus_tag="J1Y83_002285"
     CDS             complement(16344..17645)
                     /locus_tag="J1Y83_002285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter"
                     /protein_id="HBA7630431.1"
                     /translation="MLVVELIIVLLAIFLGARLGGIGIGFAGGLGVLVLAAIGVKPGN
                     IPFDVISIIMAVIAAISAMQVAGGLDYLVHQTEKLLRRNPKYITILAPIVTYFLTIFA
                     GTGNISLATLPVIAEVAKEQGVKPCRPLSTAVVSAQIAITASPISAAVVYMSSVMEGH
                     GISYLHLLSVVIPSTLLAVLVMSFLVTMLFNSKLSDDPIYRKRLEEGLVELRGEKQIE
                     IKSGAKTSVWLFLLGVVGVVIYAIINSPSMGLVEKPLMNTTNAILIIMLSVATLTTVI
                     CKVDTDNILNSSTFKAGMSACICILGVAWLGDTFVSNNIDWIKDTAGEVIQGHPWLLA
                     VIFFFASALLYSQAATAKALMPMALALNVSPLTAVASFAAVSGLFILPTYPTLVAAVQ
                     MDDTGTTRIGKFVFNHPFFIPGTLGVALAVCFGFVLGSFML"
     gene            complement(17763..19199)
                     /gene="aspA"
                     /locus_tag="J1Y83_002286"
     CDS             complement(17763..19199)
                     /gene="aspA"
                     /locus_tag="J1Y83_002286"
                     /EC_number="4.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313147.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate ammonia-lyase"
                     /protein_id="HBA7630432.1"
                     /translation="MSNNIRIEEDLLGTREVPADAYYGVHTLRAIENFYISNNKISDI
                     PEFVRGMVMVKKAAAMANKELQTIPKSVANAIIAACDEVLNNGKCMDQFPVDVYQGGA
                     GTSVNMNTNEVLANIGLELMGHQKGEYQYLNPNDHVNKCQSTNDAYPTGFRIAVYSSL
                     IKLVDAINQLREGFERKAVEFQDILKMGRTQLQDAVPMTLGQEFRAFSILLKEEVKNI
                     QRTAELLLEVNLGATAIGTGLNTPKEYSPLAVKKLAEVTGFPCVPAEDLIEATSDCGA
                     YVMVHGALKRLAVKMSKICNDLRLLSSGPRAGLNEINLPELQAGSSIMPAKVNPVVPE
                     VVNQVCFKVIGNDTTVTMAAEAGQLQLNVMEPVIGQAMFESVHILTNACYNLLEKCIN
                     GITANKEVCEGYVYNSIGIVTYLNPFIGHHNGDIVGKICAETGKSVREVVLERGLLTE
                     AELDDIFSVQNLMHPAYKAKRYTDESEQ"
     gene            19536..20012
                     /gene="fxsA"
                     /locus_tag="J1Y83_002287"
     CDS             19536..20012
                     /gene="fxsA"
                     /locus_tag="J1Y83_002287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein FxsA"
                     /protein_id="HBA7630433.1"
                     /translation="MRWLPFIAIFLYVYIEISIFIQVAHVLGVLLTLVLVIFTSVIGM
                     SLVRNQGFKNFVLMQQKMAAGENPAAEMIKSVSLIIAGLLLLLPGFFTDFLGLLLLLP
                     PVQKHLTVKLMPHLRFSRMPGGGFSAGTGGGNTFDGEYQRKDDDRDRLDHKDDRQD"
     gene            complement(20028..21284)
                     /gene="yjeH"
                     /locus_tag="J1Y83_002288"
     CDS             complement(20028..21284)
                     /gene="yjeH"
                     /locus_tag="J1Y83_002288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine/branched-chain amino acid
                     transporter"
                     /protein_id="HBA7630434.1"
                     /translation="MSGLKQELGLAQGIGLLSTSLLGTGVFAVPALAALVAGNNSLWA
                     WPVLIILVFPIAIVFAILGRHYPSAGGVAHFVGMAFGSRLERVTGWLFLSVIPVGLPA
                     ALQIAAGFGQAMFGWHSGQLLLAELGTLALVWYIGTRGASSSANLQTVIAGLIVALIV
                     AIWWAGDIKPANIPFPAPGNIELTGLFAALSVMFWCFVGLEAFAHLASEFKNPERDFP
                     RALMIGLLLAGLVYWGCTVVVLHFDAYGEQMAAAASLPKIVVPLFGVGALWIACVIGY
                     LACFASLNIYIQSFARLVWSQAQHNPDHYLARLSSRHIPNNALNAVLGCCVVSTLVIH
                     ALEINLDALIIYANGIFIMIYLLCMLAGCKLLQGRYRLLAVVGGLLCVLLLAMVGWKS
                     LYALIMLAGLWLFLPKRKTPENGITT"
     gene            21560..21853
                     /locus_tag="J1Y83_002289"
     CDS             21560..21853
                     /locus_tag="J1Y83_002289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004391824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HBA7630435.1"
                     /translation="MNIRPLHDRVIVKRKEVETKSAGGIVLTGSAAAKSTRGEVLAVG
                     NGRILENGEVKPLDVKVGDIVIFNDGYGVKSEKIDNEEVLIMSESDILAIVEA"
     gene            21897..23543
                     /gene="groL"
                     /locus_tag="J1Y83_002290"
     CDS             21897..23543
                     /gene="groL"
                     /locus_tag="J1Y83_002290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120975.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HBA7630436.1"
                     /translation="MAAKDVKFGNDARVKMLRGVNVLADAVKVTLGPKGRNVVLDKSF
                     GAPTITKDGVSVAREIELEDKFENMGAQMVKEVASKANDAAGDGTTTATVLAQAIITE
                     GLKAVAAGMNPMDLKRGIDKAVTAAVEELKALSVPCSDSKAIAQVGTISANSDETVGK
                     LIAEAMDKVGKEGVITVEDGTGLQDELDVVEGMQFDRGYLSPYFINKPETGAVELESP
                     FILLADKKISNIREMLPVLEAVAKAGKPLLIIAEDVEGEALATLVVNTMRGIVKVAAV
                     KAPGFGDRRKAMLQDIATLTGGTVISEEIGMELEKATLEDLGQAKRVVINKDTTTIID
                     GVGEEAAIQGRVAQIRQQIEEATSDYDREKLQERVAKLAGGVAVIKVGAATEVEMKEK
                     KARVEDALHATRAAVEEGVVAGGGVALIRVASKLADLRGQNEDQNVGIKVALRAMEAP
                     LRQIVLNCGEEPSVVANTVKGGDGNYGYNAATEEYGNMIDMGILDPTKVTRSALQYAA
                     SVAGLMITTECMVTDLPKNDAADLGAAGGMGGMGGMGGMM"
     gene            23681..24034
                     /locus_tag="J1Y83_002291"
     CDS             23681..24034
                     /locus_tag="J1Y83_002291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4156 domain-containing protein"
                     /protein_id="HBA7630437.1"
                     /translation="MHVKYLAGIVGAALLMAGCSSSNELSAAGQSVRIVDEQPGAECQ
                     LIGTATGKQSNWLSGQHGEEGGSMRGAANDLRNQAAAMGGNVIYGISSPSQGMLSSFV
                     PTDSQIIGQVYKCPN"
     gene            complement(24084..24953)
                     /locus_tag="J1Y83_002292"
     CDS             complement(24084..24953)
                     /locus_tag="J1Y83_002292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA7630438.1"
                     /translation="MAISIKGVNTGVIRKSNNFIALALKIKEPRNKESLFFMSAMELR
                     DLLIALESRLHQKHKLDAAARLQYEQARDKVIKKMAENIPEILVDELKNADINRRVNT
                     LELTDDQGEILNFALTLHDGSKCELVVNELQIEMLARAIIHAINNAEMRELALRITSL
                     LDFLPLYDVDCQDDGNLEYDTYSQPEWKHNLFNHYLAVLYRFKDESGNEQFSGAVVKT
                     REATPGKEVEAITRRMLDFSPRLKKLAGVPCQVYVRTVAANNAQPLTQDQCLRALHHL
                     RVQSTSKTAPQAK"
BASE COUNT         6677 a         5940 c         6093 g         6314 t
ORIGIN      
        1 taacttttgt tagcgttttg aaattaaaaa caccgttcat ctgaagagat attaattttt
       61 agcgatgatg gagggataat tatatttgat ctggcacaag ttttactgat gaacgatgta
      121 acttgtgcca gggggatttt gcattacggt aataattatt acttacagat aaccagcgat
      181 ccgttattgg caatattgtt tcagtagtga gtagtgttct gcctgaatac ggtaacggta
      241 aaccggacgc cccgtgacgc cataatggat actggtgaac aatatgtggc agttgaccag
      301 ccagataagg tatttacggc aggaaacacg cgagatgtta acctcgttag ccagctcgtc
      361 ggttgaaaac tcatagtcct gatgcgcgtc aatccactgg cacagcgtgc gtaacgtctg
      421 cggcgttaag ccttttggca agcgacgagg atcctgctcg ttggagctgc tgccgtggat
      481 tagctgatca agctcggcct ggtcataata ctgatgtttt tccagcgcca ttttcttttg
      541 ccgccagccg gtgagcgcct cttcaaagcg ggaagcctgg aagggtttga tcaggtaatc
      601 cacaacaccg taatgcagcg aatctttaat ggttactgca tcggcagcgg aggagatgac
      661 aatcacatca cttttgcaac gcgcgttatg caggacaggc agtaaatcga gcccgttctc
      721 tttttgcata tagatatcta gcaatatcag gtcgataggc gtatcgctat tgaagataat
      781 ctctttggct ttctccagcg tcgaagccgt tccacagcat tgaaagcccg ggatttgtgc
      841 cacgtatcgg cgattcagct ccgcgaccat tgcgtcatca tcgataatta atacattgat
      901 catctgttcg acctctcccc gtcccagggt atctggacaa aaaattgtgt gaaaatcccg
      961 ggttccgatt ccacggcgat gctgccgccg agattttcta cctgttgttt gacaagtgct
     1021 aaaccgacgc ctcgctcgct tccttttgtc gagacacctt tgtcaaaaat gtgatcgatt
     1081 ttgtcgggtg cgatccccgg tccatcatcg ttaacttcgc agtgcagcca gccgtgacgg
     1141 tagtgcaatg ttacgctaat ttcgccaccg ggttccggcc ctaatgcctc cagcgcgttt
     1201 tctatcagat ttcccaacgt ggtaatcagc gtcgcgacct gatcctcact accgctgtct
     1261 ggcagctggc tttcactgtt taaaatcagc gtatggccta aatcggtcgc gcggttaatc
     1321 ttgctgatta aaaaaccagc gataaccgga gatttgatct tacccagcag agagccaatc
     1381 tcttcctgat agttattggc tgttttgaga atgtaatctt ccaactgctt ataactcttc
     1441 agatgcaata atccgagaat cacatgcaat ttattcataa attcgtggga tcgttcacga
     1501 agtgcgtcag catagttgac cagaccgtcg agtcgctgca tcagtttacg tacttcagtt
     1561 ttgtccctga aggttgaaat ggcaccgatg ataacgccat tactgcgcac cggaacggtg
     1621 ttgatcagta atagtcggtc cttaatcgta atctcttcgt cgcggcgtgg agttccgtcg
     1681 cgtaacactt ccgagacatc taccacctgt gaccacgagt ggcttagcgt cgacagtttc
     1741 tcatcgtcct gcgacttacg gtaattcagc aattcttgtg cggcatcgtt gatcagcgtg
     1801 acctcgccgc gatcgtccac ggcaacgacg ccttctttga tggactgcaa catggcctgg
     1861 cgttgctcaa acagtgtgga gatttcgtag ggttccaggc cgaaaaggat ttttttcagt
     1921 acgttaacca gaatgcaggt gccgatcagt ccgaccagca tgccaaatag taccgaccag
     1981 ataatgctcc agcgactgtc attgatctgt tgggtcacgc ggcttaactc aaggccgatc
     2041 gccactacgc caatttgttt atgattttca tcgtagatgg gggtaaatac gcgtaaagcc
     2101 tgcgccagaa aaccgcgatt gatagcgaca ttttcttcgc cattcagcgc tttaaggatg
     2161 tcatcacctt taaatggctg accaatacgc tgggcttcag gatgcgagta gcgaagactg
     2221 tgcatatcgg taacgacaat aaacagcaga tcgttgcgtt tgcgcacggc ttccgcgatg
     2281 gcctggattc cactctcctg cggttttttc tgcaagccct gacggatttc cggcgagtcg
     2341 gcgagggtac gcgctactgc cagagccttg ttagctagcc cgtctcgcgt catatcactg
     2401 atttgcgaga agtaaatcag atgcaccacc aatagcaccg agaacagtac cgcgctgacc
     2461 attaagatca cagtggtact caatttcatc ggacgtttgc gtaacatacg gtagggcaat
     2521 gaatgtctca tcagcttcct tgtgtgacaa atttcttaag cattatctct gatgaggcgg
     2581 gtaattcaaa gggagtaaga atgattggct atatagggga agagactctg gcaacggaaa
     2641 ctgccagtgc tgtatgaaga ttccggggct atgcttatag tgaaaaccat actgatgaga
     2701 gagggaaggt catggatcgg gcgctactgg acgggggtta tcgctgttat accggcgaaa
     2761 agatcgatgt ctatttcaat actgcgatat gtcagcattc tggcaattgc gtacgtggca
     2821 acggcaagtt atttaatctc aaacgaaagc cgtggatcat gccggatgaa gtcgacgttg
     2881 ccactgtggt taaagtgatt gatacgtgcc cgagcggcgc gctgaaatac cgtcataaat
     2941 aagcgagggt aaaatggaaa tacgcgaagg ccacaataaa ttttacatta atgacgaaca
     3001 aggcaagcaa atcgctgaaa ttgtctttgt gccgaccgga gagaatttag cgattatcga
     3061 acataccgat gtcgatgaaa gcctgaaagg gcaagggatt ggtaaacagc tggttgcgaa
     3121 agtcgtggaa aaaatgcgtc gggaaaaacg aaaaattatc ccactatgcc catttgcgaa
     3181 acacgaattt gataaaacac gggagtatga tgatattcgc agttgatggg agagtacaga
     3241 gtcacgatat ttttcatgct ctccgcggtg tgatgcagga gagcatctga agggtagggg
     3301 gatgcacaaa gaatggtcag agagagcgtt tttttgtccc aagtcatccc ctttactgag
     3361 caaaaaaaaa agaatatctc ctatatgaga atcatcattc ggggttaata agttttgcgt
     3421 ccccagagcg ttcaatattg ataggagtca tattatggaa ggtaaaaaca agttcaatac
     3481 ttatgttgtt tcttttgatt atccatcatc ttattcctca gtgttcttaa ggttaagatc
     3541 attgatgtat gatatgaatt tctcctctat cgtggctgat gaatatggga taccacgaca
     3601 actgaatgaa aacaccttcg caataacgac atcgttagcc gcaagtgaaa tcgaagattt
     3661 aatcaggctc aaatgcttag acttaccgga tattgatttt gacctcagca ttatgacagt
     3721 tgatgactat ttccgtcagt tttacaagta gccaggaagg ataagaagaa cataaaaaat
     3781 ggcactattc tctaaaatat taatttttta tgtgattggt gtgaacatat cctttgtcat
     3841 tatctggttt atctcacatg agaaaacaca tattcgttta cttagtgcat ttctggtcgg
     3901 aataacctgg ccaatgagtc ttcctgtggc attacttttt tctctctttt aggagctgta
     3961 gttgagtgga cccgctggtt atcttgaaac gaagcgtctc tcctcaaaac tggatttgcg
     4021 gaatgacgaa cgaagcagtc gcctgactgc ttcgttcatt aaagtgaaat ttatttctgt
     4081 gggcgcatcg ccgggaagag aataacgtcg cgaatagtat gactgtttgt aaacagcata
     4141 atcattcggt cgataccaat acccagacca gcggttggcg gcagaccata ttccagcgca
     4201 gtcacgtaat cttcgtcata gaacatggct tcgtcgtcac ctgcagcttt agcgttaacc
     4261 tgttcctgga aacgttcagc ctgatcttct gcgtcgttta attcgctaaa accattaccg
     4321 atttcacgac caccgatgaa gaattcaaaa cggtcggtga tttccgggtt aacatcatta
     4381 cggcgcgcca gcggggacac ttctgccgga tattccgtaa taaaggttgg ctgaatcaga
     4441 tgtgcttctg ccacttcatc aaagatctcc gtaacaatac gtcccagccc ccagcttttc
     4501 tctaccgtaa taccgataga ttcagctaat gctttagcag catcaaaatt atccagatcc
     4561 gccatatcgg tttctggacg atattttttg attgcttcgc gcatggtgag tttttcaaac
     4621 ggtttgccga aatcaaacac atgctcgcca taagtgactt tagtggtacc cagtacctct
     4681 tgtgccaggg tgcggaacag tgactccgtc agttcaatca aatcgtggta atccgcatat
     4741 gccatgtaga gttccatcat tgtgaactca ggattatggc gaacagaaat accttcatta
     4801 cggaagttac ggttgatttc gaatacccgt tcaaaaccgc ctacaaccag acgtttcaga
     4861 tacagctccg gcgcgatacg caggtacata tctaaatcca gagcgttatg atgggtaata
     4921 aacgggcgag cagatgcccc acctggaatt acctgcatca tcggggtttc tacttccata
     4981 aagccgcgtg cgaccatgaa ctgacggata gcggccagaa tttttgaacg gacaacaaac
     5041 gtttgacggg atttatcgtt agcgatgagg tccagataac gttgacgata acggacttcc
     5101 tgatcctgca gaccatggaa tttatctggt aaaggacgta gtgctttagt cagcaggcgc
     5161 agctcagtac agtgaatgga aagctcaccc gtttgcgtct taaacagcgt accgcgggca
     5221 ccgataatgt cacccagatc ccatttttta aactgatcgt tataaacacc ttctggcagg
     5281 ctatctcttg caacgtacag ttgaatacgg ccaccgacat cctgcaacgt tacaaaggag
     5341 gctttcccca taatacgacg ggtcatcatt cggccagcaa ccgagacttc aatgtttaag
     5401 gattccagtt cctggttatc cttcgcatca aactcttcgt gcaactggtc agaggtatgg
     5461 tcgcggcgaa aatcattggg aaacgccaca ccttgctgac gtagtgccgc cagtttttcg
     5521 cggcgatttc tcagttcatc gttaaaatca atagcctcat tggctccccg tgtttctcgt
     5581 tcagacattt tggttcctct aaatccagct ttcaatttag cgtagataaa gagacagatc
     5641 ggtacactgt attatctgcc tcgactatta ataactcagt ttatcttatc gaaactaacg
     5701 tttacctcaa gttgatatca ctaataaata accataaaaa tggtttaaat gacttattaa
     5761 tctcgatttg ctagtgtgat aaactgaaag ttaatttatt cataaccaat tgtttttacg
     5821 acagttaatc gttgctctcc tgtatcatat tcgtcggcgt gctaaacaga aaacgggtag
     5881 caaaggccaa taccacgatg atcgcgacac aagccaacgt ccactctccc atctgagaaa
     5941 agaatcgctg gtaagcggca attgccatac cgctaatttg cgactctgtc gtctgctgtg
     6001 ccacgacgcc tgccagccag ttggcgactg cgcctgttgc cagcatataa ataccagtta
     6061 atacgccaga cattttcaga cgcgtgattt gcgcaatcgc caccgggtca ataaagagtt
     6121 cagcaaagcc cattagcgcc agcccggata tcatcacgcc cattgacgct tgaccgccag
     6181 ccgctgcatg tcgggcatca aatgccagca acataaagcc acaagccatc agcagtaagc
     6241 caaaggcaaa cttcagccag acgcgcaatg ctgaattgcc gcggctttct ggcgacgcca
     6301 gccaggccag aacaacccca gcgagcatca ccgcaatagc attcaccgac tggaatagtg
     6361 ctgtaggtac ttcaatattg aatgcctgac gattcacaaa gcgatcgata aacaagctga
     6421 tggtactgcc gccctgttgt gccagtaccc agaacaacgt cccgacaaac atcaacaata
     6481 caatttgcca aagagcacgg cggtgttcgg ggaatttgat catcatgcgg gcaatgattt
     6541 gtgcggcaat gaggcaaacg atcgccagca aatatccgga ccagtcgttc tccagcagca
     6601 gggtaaaaaa tactggggct aaacagagca tcaccactaa ccagctccat actggtaagg
     6661 caaatttgac gcttgtgagc gcttttttat ccatactacg cgtggactgg aaatgacgat
     6721 gaccacttaa gaaaatcaac aaaccgataa acatgccgcc tcccgcaagg gcaaagccaa
     6781 catgccatcc ataccactga gcagccaggc cgcaggcgat gggcgcggcg atagaaccga
     6841 tattgcccgc agcatacagc agcgaaaaac cgccatcacg tcgatgatcg ttctcgtcgt
     6901 agagctcgcc aagcaaacag ctgatgtttg atttgaataa accgtagcca caaataatga
     6961 ttgccagcgc cagatacagg ctaaaggttg tatttgtatc aatgcccagc accacatggc
     7021 caagggtcat taacagcgcg ccggcaatca ctgcagtgcg gttgccgagc aggcggtcgg
     7081 caagccagcc gccgagaata ggggtaacgt aaaccagaga agcatatgcg ctgaacaggc
     7141 tgatggcatg gttatcatca aaaccaagct gatgggtgag atagagaatg agtaaggcac
     7201 gcatgccgta aaaactgaag tactcccaga tttggatcgc cacgatatag tatatcgcgc
     7261 gcggctgtga gggtgttttc atgtgttctc cttatgagca aaaaagggaa gtggcaagcc
     7321 acttcccttg tacgagctaa ttattttttg ctttcttctt tcaatacctt aacggtatag
     7381 cggccatcag cctgacggta tgcaccgtga atatcggttt caaagcccgg atagtgagcg
     7441 ccgatttcac acagcatctg caggaactcc agaaccggac ggctttcttc ggtgatcatt
     7501 tcacccggca ttaccagagg aactcctggc ggatacggaa ggatcatatt ggcgttaata
     7561 cgacctacca tttcgtcgag gtaaacttct tcggtcatac cgtgcagctc tttctggaac
     7621 gcagcatacg gagtcattac catagtcggc agaacttcaa atgcgcgata catcagatcc
     7681 ggcagattgt ggtgaacaat cagtttgtgg atattctgag ccagttcctg aatacgcatg
     7741 ttttcataga attcaggatc ttcacgatac agagacggca gcatgttttt cacacgcagg
     7801 ttcaggtcga acgcacgttt gaagtcagtc agagcacgca gcaggctcag tgctttggtc
     7861 ttatcgatac cgatgctgaa caggaacagc aggttatacg gaccggtttt ctcaacaatg
     7921 atgccatgtt cgtcgaggta tttcgccacg atgctggccg gaataccaaa gtcgctcatg
     7981 gtgccgtctt tttccatccc cggagtcagc agggtgactt tgatcgggtc aagatacatg
     8041 tgctcgttat cgatgttttt gaagccgtgc caggtgctgt cagaacgcag cggccagcat
     8101 tcagtcgtat cgatatgatc cggctgccat acatcaaaga accagccatc agattccgtt
     8161 ctcagacgtt tgatctcttt acggaatttg atcgcacgtt caatggaacc attgatcaga
     8221 cgtttacctg cattgccttt catcatcgcc gcagcggttt cagtggacgc cacgataccg
     8281 tagtgcggag aggtggtggt gtgcatcatg taggcttcgt taaaggtttc ttcgtttacg
     8341 tcacctttaa cgtggatcat ggaagcctga gagaacgccg ccagcagttt gtgagtagac
     8401 tgggtttcgt aaatcacttt cccttctaca cggccaccgc tcataccgca tttaccttcg
     8461 taaatcggtg agaagttggt gtaaggcacc cacgcggagt caaagtggat ggatttcaca
     8521 tccagtgttt tcttgatgaa gtcggtgttg tacagcagac catcataggt agagttggta
     8581 attacagcat gtaccggcca ggttgcgttt ggtgtttctt tcacgcgctt agcaatggta
     8641 gcgtgctgga attcactctg tgggatacca ccaagaatac cgtaagcgtt acgggtcggg
     8701 cggaaataga ttggcgtaac atcgctcatc atcatcaggt gggtcagcga tttgtggcag
     8761 ttacggtcaa tcagaatggt gctgcctgcc ggagcagagt acataccaac aattttgttc
     8821 gcagtggaag taccgttggt caccatgtag ctgcggtctg cgttaaagac gcgagcgata
     8881 tactgttctg cttctttgtg tggaccactg tgatccagca gagaacccaa ttcagatact
     8941 gaaatggaaa tatcagattt catggtgttc ggaccaaaga aatcatagaa caggctacct
     9001 accgggcttt tctggaatgc agtaccaccc atgtgaccag gagtacagaa agtatattta
     9061 ccttcacgaa catatttaaa cagtgctttg gtcagcggag gcagaatagt gttgatatat
     9121 tcgtcagtgg tctgcttgat cttgttagca atatcatcag cagcacccag cgcatattca
     9181 aagaagctaa tctgtaaacg taggtcattc aggcttacat cgagagtgga atacgtatta
     9241 gcgaacgcgt acaacggcag gttctcgttc attttgctaa tttcttcgca cagctcgaga
     9301 ttatatttat cccagtcaaa aataacgccg cacagacgcg cattgttttc gatcagtttt
     9361 aataagtcgt cacggtcgtt cgggtaaaca atctggaagt tcagacgttc aagcgcgcga
     9421 tgaagttcac ggatgggttc ttctttaaaa taaaccccca tgtgattcaa tattgcaata
     9481 acgttcatag tcatatctcc aggtaaaaaa ggcccctccc aacacatggg acaaaatgaa
     9541 aggaggagcc tcggaaaata cttttaatta atgtgcgtta gacgctgtgt ggttatccat
     9601 tgagtggctc tggcgctcgt gcattttgcg agcgtagaac atcaggataa tcaggctgac
     9661 gatgaaggta cctgccagct cgaaggagct tgcgcccatc agcgcgatga agcagaacac
     9721 gcaacccagt acagagcaga tcaggctgac aaagttgcgg atgttaacgc cttcaaaacg
     9781 aatcaggtca acgcaagagt agaagtacgg cagcatagtc agcagtactg cgataccggt
     9841 cagttcaccg aacaggtcag atgctttacc accggcagag ttcatcagag tgatgaggat
     9901 catcagggca gtcattttca ctgcagccag cagcagacct tttttcggaa taccgttgct
     9961 gtcgacttca ccataaactt tcgggaagtt accgtcgtta gcggcacgta cacctgcctg
    10021 gcctaccaac atcatccagg agcccagaga agtcaggcac gcaaaggcgg tgaatgcaga
    10081 aaccagtggt gcagcccagt taccgaggat agttgaagca ctgattgcaa acggagcacc
    10141 ggaagccgcc attacagaag acggatacat accggaaagc acctgagtcg cagcgatgta
    10201 aacaatacct gctaaaccag tacccagcat ggttgccagc ggaacggtac gtttcgggtt
    10261 tttaaccata ccagtactta ctgctgcgga ttcaacaccc acgaaggccc acaggcagag
    10321 cagaatactt ttaatgatcg catgaccatc agtggtatcc gcagtattcc agttagctgc
    10381 ataagttgct gcatcaaacc aatgccagcc aacaatagca gtcatcacca caggaataag
    10441 aaccagcacc agaccaatcg tggttaaacg gcttacccag gtaccgccga gcatattcac
    10501 aaaggtaaat acccagacga tagcaataca ggcgataccc gccggaacag gatcatttaa
    10561 tactgggaag aaggtagaaa gataagatac agcggtaata ccaattgcca ggttaccaat
    10621 ccagttagca tggtaataaa gaacacctgt ctgaaaacca aatgcaggag aaatttctcc
    10681 ggcatacgca attgggccac cttgttgcgg gttttttgtt gccagtcggg catatacata
    10741 tgccagcgac attgcaccaa taatagagat aatccaaccc cagatagcaa taccaccgat
    10801 acttgctagg ttcgcaggta ataatgcaat accgctcccc atcatattac cggcaacaac
    10861 accggtacag gcaaatagcc cgatcttctt ggcagaactc atgctcttct cctaatttca
    10921 tttttgaatt tggagtccgg gtcatgatgt ataactattt cctgaccaga ccaaactggc
    10981 gctaagatta ctcacgaaaa aaggattaat cctaaagatt aggtgaaata acacaaaagt
    11041 ttctgtaagt gagaacttga ggttttttat taacacatca ggatcgcaag ttgatatcat
    11101 gaaaagataa acattcaata tttacaatgt gttatgtgac atcttctggt taaatttatg
    11161 taataaaaat tatgcggcaa ataaattgcc gcaacatatt ataccaacgg gaacatacaa
    11221 aaactcaaca acaaatattc ccgagcatga atcaacccgg gagttactta ttctgaagcg
    11281 agaaatttgt cgagataagg tacaacataa ggaacagaag tctggaatat accattttca
    11341 atccagtaaa gggtgtttgc ccctgggcgt aaattaaagg cggtgagata tgcatcagct
    11401 gcttcccggt tcatcccctt catttcataa accttgccaa gcaacacata atttagccag
    11461 gacatttcaa gatcaatgcc agtatttatc gcctgataag actcatctgt tttacctttt
    11521 accagggcac tgaccgcttt tatttgatat ataattgaca ggttgttcaa ttccggcagt
    11581 gtaacaatgt tatctatttc tgtgttcagt gctgctaatt gtttttcgtc taaaggatgt
    11641 tgagaatggc gcacgatatc aactaatgct ttttctgctc tcgcgtaggt aaattctggg
    11701 gatgattgaa caatctcacc taataattca ctggcacgat ccaatgattt atcatcacca
    11761 tgcagtaaat aatcatgtgc ctgataaaaa ttagttaata acgcaccacg atgcggcaaa
    11821 atttgctgga gcatctcctg cattcgttgt ggccacggtt ggtttaacgc ttttgataaa
    11881 ctctggagta aatcattttg aatcgccagc tgattaccgt tagtgatgac ataacgttta
    11941 tccagcatgg ttgaactatc tgcattgtct accaatttta ccgacataaa gcattgttga
    12001 gcacggtatt ggcgctgatt aacaaacgca atagataatg ttttaccgga actgctcggt
    12061 tcatcaatgt tgtagttgat tttgtcatgc accataaagg tggagaaggt gttaagtgat
    12121 gtcgccacca aatcacccac gcctatcgcg taagagagct gatacgggga actccagctg
    12181 ttacaactct tattaaccat attaatgtca atatcgcgtg gattgagcaa aatgcgcgat
    12241 ttactcatag gaagacgtgt ttcaagactt gaaaacgcta ccagtgcgac acagatacct
    12301 aacgacaaca ggaaaaaaaa ccatacccaa aaggtagtga atcgtttgct tttaactggg
    12361 gattgttcag gtggcgttgt ggtgttttga atgttaagac tgtgggaggg agaatctgtg
    12421 gcaggaaccg cctctggtat agggggaggc gaagatagca ttaattcctc tccctcttct
    12481 tcgctgtacc agataaccgg caccattaat ttatagccgc gctttggtac agtagcgata
    12541 tagacaggac tatcttcatc attatctttt aatgacttac gtagttctga gatactctgc
    12601 gtcacaacgt gattggtgac aatacttctc ttccagacat tatcgataag ttcatccctg
    12661 ctaagtactt cgccactgtg ttgagcaaag aaaaccagaa gatcgattaa tctcggctca
    12721 agggtaagtt gacgcccatt gcggctaatt tggtttatgg acggagtaac aagccattcg
    12781 ccaacgcgaa ctacaggttg ttgcataata gaaactcatt cgaaaaggga atgatgcaat
    12841 gataattgcc ataacctatt ttttccatct atagatgggt ttatttacat attattggtg
    12901 aatgcaagac gttattttta ctcgccataa acttcttgat tacatagtat tacgaaagga
    12961 ttttactgag aaccagaagt aattttcctt accatcaaaa ttcatcatct ttgccaaaga
    13021 aaaatgttca gaaaataatc catgaaaaat tgtccggagc gcttactatt ttaatggatt
    13081 gttagtcttt gcatgagcaa acggactgat acatttctct ttgttctcat tcagaaaatc
    13141 tcatcagttg gcgttctgcc cgatgttgtg ctttatgggt gcgttacgct cctgatgacg
    13201 tcatttgacg ttcaacagca tcacggggcc gcacgacatt tcacgtcagt cagcgctata
    13261 gctcagaaac aaattttccc gaattgggat atgcccgcaa attgctggtg atgtggggag
    13321 aatctggttg agttcggtag aattgatttg gcggcaggac gcttaaatcg tggcgtcctg
    13381 aaacgaaaaa cggacctccg tggaggtccg tttatatgaa tttggtgccc ggactcggaa
    13441 tcgaaccaag gacacgggga ttttcaatcc cctgctctac cgactgagct atccgggcaa
    13501 cggagcgcat taaaccgtaa tctgcacatc tcgtcaacct aatttcagta aaagcgattc
    13561 aactgcttaa gattgcggca aatcgcttct tttctgtgtt tctgtcaggt cagtgcacca
    13621 ccctgacggc agcgggcgaa gcgcaggata tcttccgcca gccgatgtgc ggtgtcgaca
    13681 tccgcctggc tacgattcac cagcattcgg cttaaacagc cttccagcac cagttccatc
    13741 tgctttgcta ccatcgccgg atcgtcaact tccagcgtgg ttaacagttc gtgggtgaaa
    13801 tcgtaggccg cgcttttttg ctgatcggcc agttgatgaa tagggtggcc aggatcgggg
    13861 taaaacgtac aggcagcgat aaacagacag cccggatagc ggttgttttt aacgcactcc
    13921 gataacgcct gataacgtgc cagcaacttt tgttcggcgg tttgcgtttc gtccagcatc
    13981 agctgacgac gccagacatc tatctgttgg ctaagataac gcagcgcatc gtagaggatt
    14041 gcctctttgt ctggccagaa gcggcgtagc tcgtccagtg gataatccac acgttcagca
    14101 accatctcca gcgtggtgtt ggcaatccct tgtaattcta ataatttcag ggcttctccc
    14161 agtacatctt cacgttgcac gctattttcc tccgtctttc ccactgcaag tgtcgttcac
    14221 ggttggcgat cgcgcaaatg tgcgctgaag gtttcagcat ccataaagcc cgtgacgcgt
    14281 gcttgtggat gctcctggcc ttgtccgtca aaaaagagaa ttgtcggtag gccaaggaca
    14341 ttaagatgct ttaacagcgc cacatcttgt gcgtcgttgg cagtgacgtt ggcctgaagt
    14401 aagaccgtat ctgctaacgc tttttgcacc tgcgggtcgc tgaaggtgta tttctcaaac
    14461 tctttacagg cgacgcacca gtcggcatag agatctaaca tcactggttt gcctttggct
    14521 tcaacgagcg tctgatttaa ctcatctacc gttttgattt gtgtaaagtt gagatgcgtc
    14581 tgagtttgcg cggtatgtgt tgcaccaaat gcccaatcct gaagtgggcg tacgctaacc
    14641 aatgccgctg ccagcaggat tatttgcacc acgcgcatcc agccgcgttt ggcctgtagg
    14701 ctggtgataa aggcccagcc aaagaatgcg acaccaagcg ccgaccacaa gcgtaatccc
    14761 catatatcac caatcactcg ctccagcagg aagaccggca gtgcgaggat cacaaaacca
    14821 aacgcggttt tgacttgttc catccacggg ccgcttttcg gcagcaagcg gttaccaaag
    14881 acggtaatta gcatcagcgg caggcccatg cccagcgcat aaagataaag cgtgccgccg
    14941 cccagccaca tgttcccgct ttgggcgata tacagcagaa tcgcgctaag cggtgcagtg
    15001 gtgcatggtg aacagatcag tccggcaatc gcccccataa taaacacacc gccaggtgag
    15061 ccgccctgtt ggcgattgct catcagcgtg agacgtgttt gcagcgaaga ggggagttgc
    15121 agagtaaaca agccaaacat tgacatcgcc agcaaggtaa agacgatggc gaggccaatg
    15181 agcacgtatg ggtgctgtag cgccgcctgg aactgtaacc ctgcggcggc aaccaccaga
    15241 cccagcgccg tgtaagtcag cgccatcccc tgcacataaa taaaggtcag caacaatgct
    15301 ctggcagtgg aaagccgctg tttaccgccc agcacgatgc cagaaatcag tgggtacatt
    15361 ggcagcacgc atggcgtaaa ggcgatacca ataccgatca acaacgccca gagcgcggaa
    15421 aagggcaatt gcgcggtggg ctgctcttgc tgcggaacag acacaggctg tgacgcttcg
    15481 ttgttggcga ccacttcgct taacggaacg gttttggttt ctggcggata acagaaaccg
    15541 gcatcagcac agccctggta ggtgacagtt aacgttgctc ccgcactcgc ctggttgatg
    15601 gttacgggaa gcgtcagccg atcgcggtaa atctcgcttt tgccgtaaaa ctcatcttca
    15661 tgccagacgc cttgcggcag ctgcacgtca gcaattttcg cgtgttccgg cgtaatgcgg
    15721 atctgtttac ggtagaggta gtaaccgtct ttgatctgcc aggtcagatt cagatcatgt
    15781 tgattttgct gaaaatcaaa agcaaaggct tgatccgcgg ggacaaattg tgaacgtccc
    15841 ggcgcgtcga ataatccggc aaaaacggaa gtgctgcaaa gtagcaggat cagcgtaaag
    15901 atgcgttgag ccatgagagg taatctgtgt ctccgtgtgt aacaggtaaa accagaagtt
    15961 ccggggtttg atatggatga tgagacttca ggcattccag cagtgcctgc tggtgagata
    16021 cggtagtttt taaaatcatc tgcacttcgt attcttgctc cagcttacct tcccagtaat
    16081 agagagaggt agcgccgggg atcaaggtcg cgcaggccgc cagtttttcc gccagcactt
    16141 tggcggctaa atcctgggct gtcgcttcat ccggtgccgt acatagcacc acgacagacg
    16201 tggtattcga acttttttca tcaagcataa acacctcgca agaacagatg gaaccgcaag
    16261 agagaggtca ctatacaacg gacggggaaa ggatgttagt cggcgggaaa gaaagcgggg
    16321 cgtgaacgcc ccgcgatggg tcattacagc atgaagctac ccagcacgaa gccgaagcaa
    16381 acggccaggg caacacccag agtacccggg atgaagaacg gatggttgaa gacgaattta
    16441 ccgatacggg tagtacccgt gtcatccatc tgtaccgcag caaccagtgt cgggtaggtc
    16501 ggcagaatga acagaccaga caccgcagca aaagaagcaa cagcggtcag cggagaaacg
    16561 ttcagtgcca gagccatcgg catcagtgct tttgcggttg cagcctgaga gtacagcaga
    16621 gcagaagcaa agaagaagat gacggccagc agccacggat gaccctgaat cacttcacca
    16681 gcggtatctt tgatccagtc gatgttgttg gaaacgaaag tatcgcccag ccacgcaaca
    16741 cccaggatac aaatacaggc gctcatacct gctttgaagg tgctggagtt gagaatgttg
    16801 tcggtatcga ctttacagat aacggtggtc agagttgcaa cgctgagcat gatgatcagg
    16861 attgcgttgg tggtgttcat cagtggtttt tcaaccagac ccatgcttgg gctgttgatg
    16921 attgcataga taaccacgcc aactacgccc agcaggaaca gccagacgga cgtttttgca
    16981 ccggatttga tttcaatctg cttttcaccg cgcagttcaa ccaggccctc ttccagacgc
    17041 ttgcgataaa tcggatcgtc agagagtttg gagttgaaca gcatagtgac caggaaggac
    17101 atcaccagaa ccgccagcag ggtggacggg atgaccacgg agagcagatg gaggtagctg
    17161 atgccatgac cttccatcac ggaagacatg taaaccactg ccgctgagat tggcgatgcg
    17221 gtgatcgcaa tctgcgcgga taccactgca gtagacagcg gacggcaagg tttaacgcct
    17281 tgttccttcg caacttcagc gataactggc agtgtcgcca gagagatgtt gccagtacca
    17341 gcaaagatag tcaggaaata ggtcacgatc ggtgcgagga tcgtgatgta tttcgggtta
    17401 cggcgcagca gcttttctgt ctgatgaacc agatagtcca gaccgccagc aacctgcatg
    17461 gcagaaatag cggcgataac cgccatgata atggaaatga catcgaacgg gatgttaccg
    17521 ggtttaacgc caatagcggc aagaaccagc acccccaatc cgcctgcaaa accaatacct
    17581 attcccccca atctggcgcc caagaagatc gccagcaaaa ctatgatgag ttctacaact
    17641 agcatattag ccttccttgt tttttaacaa gttgatatta aattgttatt tttaagttac
    17701 tgctcacaag aaaaaaggca cgccatctga cgtgccttct ttatttgtac taccctgtac
    17761 gattactgtt cgctttcatc agtatagcgt tttgctttgt aagccgggtg catcagattc
    17821 tgtacggaga aaatatcgtc aagttccgct tcagtcaaca gaccgcgttc cagaacgact
    17881 tcacgtacac tcttaccggt ttcggcacag attttaccca cgatgtcacc gttgtggtga
    17941 ccgatgaacg ggttcaggta agtaacgata ccgatagagt tgtaaacgta accttcgcac
    18001 acttctttgt tagcagtgat gccgttaatg catttttcca gcaggttgta gcaagcgttg
    18061 gtcagaatgt gaacggattc gaacatggcc tggccaatga ctggctccat aacgttcaac
    18121 tgcagctgac ctgcttctgc tgccatggta acagtggtgt cgttaccgat gactttgaag
    18181 catacctggt taaccacttc cggaacaacc gggtttactt tagctggcat gatggaagag
    18241 cctgcctgca gttccggcag gttgatctcg ttcaggccgg cacgtggacc agaagagagc
    18301 aagcgcaggt cgttacagat tttggacatc ttcacagcca ggcgtttcag cgcgccgtga
    18361 accataacat aagcgccgca gtcggaggtc gcttcgatca ggtcttcagc cggtacgcat
    18421 gggaagccag tgacttcagc cagttttttc actgccagcg gagagtactc tttcggcgtg
    18481 ttcagaccag taccgattgc tgttgcgcca aggttaactt ccagcagcag ttcagcggta
    18541 cgttggatat ttttcacttc ttctttcagc aggatgctga aggcgcggaa ttcctgaccg
    18601 agggtcatcg gtactgcgtc ctgcagctgg gtacgaccca ttttcaggat gtcctggaat
    18661 tcgacagctt tacgttcaaa gccttcacgc agttggttaa tcgcatctac cagtttaatc
    18721 agggaagagt aaactgcgat acggaaaccg gtcgggtagg cgtcgttagt ggactgacat
    18781 ttgttaacat ggtcgttcgg gttcaggtac tgatattcac ctttctggtg acccatcagt
    18841 tccagaccga tattggccag cacttcgttg gtgttcatgt ttacggaagt acctgcgccg
    18901 ccctggtaga cgtctaccgg gaactgatcc atgcattttc cgttgttcag gacttcatca
    18961 catgcggcaa tgatggcatt cgctacactt ttaggaatgg tttgcagctc tttgtttgcc
    19021 atagctgcgg cttttttaac cattaccata ccgcgaacaa attcaggaat atcactgatt
    19081 ttgttgttgc tgatatagaa gttttcaatc gctctcagag tgtgaacacc atagtaggca
    19141 tcagctggaa cttccctggt acccaacaga tcttcttcga tacgaatgtt gtttgacatg
    19201 tgaaccttct ttttcaagct gccaatgatt tgctttaaac acacagaata tatgtggttt
    19261 cgaatgtttt tcgaccgacg attatcccct gcatcgaccg aatacccgag atcatatgct
    19321 gcttgaggat ttctaccgta acctggatca ctttaagtgt cggtttttac cccttaatta
    19381 ttaatttgtg aaatggatca ccgctttggg attactacca aaaatagttg cgcaaacatc
    19441 ttgaaatttt gctaatgacc acaatataag ctaaacgcga ttcgcaaccc attcaggtag
    19501 ccgggtttaa ccggctgcta ttacaggaga aacctttgcg ctggttacct tttattgcca
    19561 ttttccttta tgtctatatt gagatttcaa tctttattca ggttgctcat gtattggggg
    19621 tattgctgac cctcgtactg gttatattca cgtcagttat cggtatgtca ctggtacgta
    19681 accagggctt taagaatttc gtgctgatgc agcaaaaaat ggcggcgggt gaaaacccag
    19741 cggcggagat gattaaaagt gtttcgctga tcattgccgg tttgctgctt ttattaccgg
    19801 gctttttcac cgatttcctc ggtttgcttc ttttattacc gccggtgcaa aagcatctga
    19861 cagtgaagtt gatgcctcat ctgcgttttt cccgtatgcc aggcggcggt tttagcgccg
    19921 ggaccggtgg cggtaatact tttgatggtg agtaccagcg aaaggatgat gaccgcgacc
    19981 gccttgatca taaagacgat cgccaggatt aatgtcgaaa cgccggatta tgtggttatg
    20041 ccattttccg gcgtttttcg ttttggcaga aacagccata accccgccag cataatcagc
    20101 gcatagagac ttttccagcc gaccattgcc agtaacagaa cgcataacag cccgccaacc
    20161 accgccagta gtcgataacg tccttgcaat aatttacaac ctgccagcat gcataacaga
    20221 taaatcataa taaagatgcc attggcatag atgataagtg cgtccagatt gatctctaaa
    20281 gcatgaatca ccaacgtgct caccacgcag cagccgagca ccgcattgag ggcattattc
    20341 ggaatatggc gagaagagag gcgtgccagg tagtggtcag gattatgttg cgcctgcgac
    20401 cagaccaggc gggcgaagct ctgtatataa atgttgagac tggcaaagca ggccagatag
    20461 ccaattacgc aggcaatcca taacgctcct acaccgaata acggcactac aattttggga
    20521 agcgatgctg ccgccgccat ttgttcacca taggcgtcga agtgtaagac gactaccgta
    20581 cagccccaat agactaatcc tgccagcagc aggccaatca ttaaagcacg aggaaaatca
    20641 cgctctggat ttttaaattc cgaggcaaga tgggcaaatg cttccagacc gacaaaacac
    20701 cagaacatca ctgataacgc agcgaataac ccggtaagtt cgatatttcc tggcgcaggg
    20761 aaggggatat tcgcaggttt gatatcgccc gcccaccaga tagcgacaat cagtgcgacg
    20821 ataagcccgg caataactgt ttgtagatta gcactggaac tggcacctcg agtaccgata
    20881 taccacacca gcgccagcgt accgagttct gccaacaaca gttgcccgct atgccagcca
    20941 aacattgcct ggccgaatcc ggcagcaatt tgtagcgcgg caggcaaacc cacgggaatg
    21001 accgataaaa acaaccagcc ggtgactcgc tcaagccgcg aaccaaacgc cataccgacg
    21061 aagtgtgcga cgccgcctgc gctgggatag tggcgaccca gaatcgcaaa cacaatcgca
    21121 atcgggaaca ctaagataat caaaacgggc cacgcccaca ggctgttatt gcctgctact
    21181 agcgcagcta acgcaggaac ggcaaacacg ccagtgccta ataatgacgt cgatagcagg
    21241 ccaatgccct gggccagccc cagttcttgt ttgagtccac tcatgggttg atgtccgatt
    21301 gcgcccaaat tatgggcaac tgcgtagatt ttcgatggta gcacaatcag attcgcttat
    21361 gacggtgatg aagaaattgc gatgaaatgt gaggtgaatc agggttttca cccgattttg
    21421 tgctgatcag aatttttttt ctttttcccc cttgaagggg cgaagcctca tccccatttc
    21481 tctggtcacc agccgggaaa ccacgtaagc tccggcgtca cccataacag atacggactt
    21541 tctcaaagga gagttatcaa tgaatattcg tccattgcat gatcgcgtga tcgtcaagcg
    21601 taaagaagtt gaaactaaat ctgctggcgg catcgttctg accggctctg cagcggctaa
    21661 atccacccgc ggcgaagtgc tggctgtcgg caatggccgt atccttgaaa atggcgaagt
    21721 gaagccgctg gatgtgaaag ttggcgacat cgttattttc aacgatggct acggtgtgaa
    21781 atctgagaag atcgacaatg aagaagtgtt gatcatgtcc gaaagcgaca ttctggcaat
    21841 tgttgaagcg taatccgcgc acgacactga acatacgaat ttaaggaata aagataatgg
    21901 cagctaaaga cgtaaaattc ggtaacgacg ctcgtgtgaa aatgctgcgc ggcgtaaacg
    21961 tactggcaga tgcagtgaaa gttaccctcg gtccgaaagg ccgtaacgta gttctggata
    22021 aatctttcgg tgcaccgacc atcaccaaag atggtgtttc cgttgctcgt gaaatcgaac
    22081 tggaagacaa gttcgaaaat atgggtgcgc agatggtgaa agaagttgcc tctaaagcga
    22141 acgacgctgc aggcgacggt accaccactg caaccgtact ggctcaggct atcatcactg
    22201 aaggtctgaa agctgttgct gcgggcatga acccgatgga cctgaaacgt ggtatcgaca
    22261 aagcagttac cgctgcagtt gaagaactga aagcgctgtc cgtaccgtgc tctgactcta
    22321 aagcgattgc tcaggttggt accatctctg ctaactccga cgaaaccgta ggtaaactga
    22381 tcgctgaagc gatggacaaa gtcggtaaag aaggcgttat caccgttgaa gacggtaccg
    22441 gtctgcagga cgaactggac gtggttgaag gtatgcagtt cgaccgtggc tacctgtctc
    22501 cttacttcat caacaagccg gaaactggcg cagtagaact ggaaagcccg ttcatcctgt
    22561 tggctgacaa gaaaatctcc aacatccgcg aaatgctgcc ggttctggaa gctgttgcca
    22621 aagcaggtaa accgctgctg atcatcgctg aagatgttga aggcgaagca ctggcaactc
    22681 tggttgttaa caccatgcgt ggcatcgtga aagttgctgc ggttaaagct ccgggcttcg
    22741 gcgatcgtcg taaagctatg ctgcaggata tcgcaaccct gactggcggt accgtaatct
    22801 ctgaagagat cggtatggag ctggaaaaag caaccctgga agacctgggt caggctaaac
    22861 gtgttgtgat caacaaagac accaccacca tcatcgatgg cgtgggtgaa gaagcggcaa
    22921 tccagggccg tgttgctcag atccgtcagc agattgaaga agcaacttct gactacgacc
    22981 gtgaaaaact gcaggagcgc gtagcgaaac tggcaggcgg cgttgcagtt atcaaagttg
    23041 gtgctgctac cgaagttgaa atgaaagaga aaaaagcacg cgttgaagac gccctgcacg
    23101 cgacccgtgc tgcggtagaa gaaggcgtgg ttgctggtgg tggtgttgcg ctgatccgcg
    23161 tagcgtctaa actggctgac ctgcgtggtc agaacgaaga ccagaacgtg ggtatcaaag
    23221 ttgcactgcg tgcaatggaa gctccgctgc gtcagatcgt cctgaactgc ggcgaagaac
    23281 cgtctgttgt tgctaacacc gttaaaggcg gcgacggcaa ctacggttac aacgcagcaa
    23341 ccgaagaata cggcaacatg atcgatatgg gtatcctgga tccaaccaaa gtaacccgtt
    23401 ctgctctgca gtacgcggct tctgtggctg gcctgatgat caccaccgag tgcatggtta
    23461 ccgacctgcc gaaaaacgat gcagctgact taggcgctgc tggcggtatg ggcggcatgg
    23521 gtggcatggg cggcatgatg taattgccct gcacctcgca gaaataaaca aacccccggg
    23581 cagaaatgtc tgggggtttt tcttttggtc atctttctag tataagattc agacacggac
    23641 gacgcgagtg gcgtccagct cattgattat ggggaataac atgcacgtaa aatacttagc
    23701 agggattgtc ggtgccgcgc tactgatggc gggttgtagc tccagcaacg aattgagtgc
    23761 tgccggtcag agtgtacgca ttgtggacga gcaaccaggt gcagagtgcc agctgattgg
    23821 tactgcgaca ggtaagcaaa gcaactggct ttccgggcaa cacggagaag aaggcggttc
    23881 tatgcgcggc gcagcaaacg atctgcgcaa ccaggcggct gcaatgggcg gtaacgtgat
    23941 ttatggcatc agcagcccgt cgcagggaat gttgtccagt tttgtcccga cggatagcca
    24001 gattatcggt caggtttata agtgcccgaa ctgatgtgat ccagaggctg gatacttcca
    24061 gcctcgtagt aaaacagatg agattatttc gcctgtggtg cagttttgct ggtggattga
    24121 acacgtaagt gatgcagcgc gcgtaagcat tgatcctggg ttagcggttg ggcgttattt
    24181 gccgctacgg tgcgaacata gacctgacat ggcacacccg cgagtttttt cagtcggggg
    24241 ctgaaatcaa gcattcggcg agtgatcgcc tccacttctt tacccggcgt tgcttcacgc
    24301 gttttcacca ctgcgccgct aaattgttca ttgccacttt catctttaaa gcggtaaagc
    24361 accgccagat aatggttgaa caagttatgt ttccattccg gttgggaata ggtgtcatat
    24421 tccagattac catcatcctg gcagtcgaca tcatataacg gcaggaaatc taacagtgag
    24481 gtaatacgta acgccagttc acgcatttct gcattattaa ttgcatgaat gattgctcgc
    24541 gccagcattt caatctgcaa ttcattgacc actaattcgc atttgctgcc atcatgtagc
    24601 gttaatgcga aattaagaat ttcgccctga tcatcggtaa gttccagcgt gttaacccga
    24661 cgattaatat cggcattttt aagctcatca accagaattt ctgggatatt ttctgccatt
    24721 ttcttaatga ctttatcgcg agcttgttca tactgaaggc gtgcagcagc atccagttta
    24781 tgcttttgat gcagacgact ttccagcgca atcagaaggt cgcgcaattc catggcagac
    24841 ataaagaaca acgattcttt attgcgaggt tctttaattt tcagcgccag tgcaatgaaa
    24901 ttattactct tgcgaataac accggtatta acacccttga tgcttatggc catgtaggtt
    24961 ctccctaacc atttctcaat taaataatta attttagttt ataagccaga taaatgggct
    25021 tggt
//
LOCUS       DADSYM010000067        24709 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2415
            SAMN08039579-rid11723173.denovo.067, whole genome shotgun sequence.
ACCESSION   DADSYM010000067 DADSYM010000000
VERSION     DADSYM010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039579
            Sequence Read Archive: SRR6362533
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 24709)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24709)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980162.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 03:39:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,484
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 4,347
            CDSs (with protein)               :: 4,347
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 43 of 85
            Pseudo Genes (incomplete)         :: 37 of 85
            Pseudo Genes (internal stop)      :: 19 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24709
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039579-rid11723173.denovo.067"
                     /strain="LGE2415"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8302 N 95.9628 W"
                     /collection_date="2015-08-24"
     gene            13..1095
                     /locus_tag="J1Y83_002293"
     CDS             13..1095
                     /locus_tag="J1Y83_002293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpD"
                     /protein_id="HBA7630439.1"
                     /translation="MKKLTVAISAVAASVLMAMSAQAAEIYNKDSNKLDLYGKVNAKH
                     YFSSNDADDGDTTYVRLGFKGETQINDQLTGFGQWEYEFKGNRAESQGSSKDKTRLAF
                     AGLKFGDYGSIDYGRNYGVAYDIGAWTDVLPEFGGDTWTQTDVFMTGRTTGVATYRNN
                     DFFGLVDGLNFAAQYQGKNDRTDVTEANGDGFGFSTTYEYEGFGVGATYAKSDRTNDQ
                     VIYGNNSLNASGQNAEVWAAGLKYDANNIYLATTYSETQNMTVFGNNHIANKAQNFEV
                     VAQYQFDFGLRPSVAYLQSKGKDLGAWGDQDLVEYIDVGATYYFNKNMSTFVDYKINL
                     IDKSDFTKASGVATDDIVAVGLVYQF"
     gene            complement(1597..2394)
                     /locus_tag="J1Y83_002294"
     CDS             complement(1597..2394)
                     /locus_tag="J1Y83_002294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBA7630440.1"
                     /translation="MLAKDKSNLKIEEIRMHKHHEIHRVKPLMPALCRIRQGKKVINW
                     ETHSLTVDNNQIILFPCGYEFYIANYPEAGLYLAEMLYYPIDLIEKFQKFYAITDQIR
                     NTTGFCLPQNPELIYCWEQLKTSIFRGFSTQIQEHLAMGVLLSLGAHHVNCLLLSDSK
                     QSLISRCYNLMLSEPGTKWTANKVARYLYISVSTLHRRLASEGISFQSILDDVRLNNA
                     LSAIQTTVKPISEIARENGYKCPSRFTERFHNRFKITPRELRKASRE"
     gene            complement(2404..2955)
                     /gene="ybcL"
                     /locus_tag="J1Y83_002295"
     CDS             complement(2404..2955)
                     /gene="ybcL"
                     /locus_tag="J1Y83_002295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Raf kinase inhibitor-like protein YbcL"
                     /protein_id="HBA7630441.1"
                     /translation="MKKLIVSSVLAFITFSAQAAAFQVTSNEIKTGEQLTTSHVFSGF
                     GCEGGNTSPSLTWSGAPEGTKSFAVTVYDPDAPTGSGWWHWTVANIPATVTYLPTDAG
                     RRDGTKLPTGAVQGRNDFGYAGFGGACPPKGDKPHHYQFKVWALKTDKIPVDSNSSGA
                     LVGYMLNANKIATAEITPVYEIK"
     gene            complement(3124..3456)
                     /gene="emrE"
                     /locus_tag="J1Y83_002296"
     CDS             complement(3124..3456)
                     /gene="emrE"
                     /locus_tag="J1Y83_002296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMR family multidrug efflux protein EmrE"
                     /protein_id="HBA7630442.1"
                     /translation="MNPYIYLGGAILAEVIGTTLMKFSEGFTRLWPSVGTIICYCASF
                     WLLAQTLAYIPTGIAYAIWSGVGIVLISLLSWGFFGQRLDLPAIIGMMLICAGVLVIN
                     LLSRSAPH"
     gene            complement(3801..4115)
                     /gene="fliE"
                     /locus_tag="J1Y83_002297"
     CDS             complement(3801..4115)
                     /gene="fliE"
                     /locus_tag="J1Y83_002297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707823.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein FliE"
                     /protein_id="HBA7630443.1"
                     /translation="MSAIQGIEGVISQLQATAMSARAQESLPQPTISFAGQLHAALDR
                     ISDTQTVARTQAEKFTLGEPGVALNDVMTDMQKASVSMQMGIQVRNKLVAAYQEVMSM
                     QV"
     gene            4330..5988
                     /gene="fliF"
                     /locus_tag="J1Y83_002298"
     CDS             4330..5988
                     /gene="fliF"
                     /locus_tag="J1Y83_002298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body M-ring protein FliF"
                     /protein_id="HBA7630444.1"
                     /translation="MNATAAQTKSLEWLNRLRANPKIPLIVTGSAAVAVMVALILWAK
                     APDYRTLFSNLSDQDGGAIVSQLTQMNIPYRFSEASGAIEVPADKVHELRLRLAQQGL
                     PKGGAVGFELLDQEKFGISQFSEQVNYQRALEGELSRTIETIGPVKGARVHLAMPKPS
                     LFVREQKSPSASVTVNLLPGRALDEGQISAIVHLVSSAVAGLPPGNVTLVDQGGHLLT
                     QSNTSGRDLNDAQLKYASDVEGRIQRRIEAILSPIVGNGNIHAQVSAQLDFASKEQTE
                     EQYRPNGDESHAALRSRQLNESEQSGSGYPGGVPGALSNQPAPANNAPISTPPTNQNN
                     RQQQASTTSNSGPRSTQRNETSNYEVDRTIRHTKMNVGDVQRLSVAVVVNYKTLPDGK
                     PLPLSNEQMKQIEALTREAMGFSEKRGDSLNVVNSPFNSSDESGGALPFWQQQVFIDQ
                     LLAAGRWLLVLLVAWLLWRKAVRPQLTRRAEAVKTVQQQAQAREEVEDAVEVRLSKDE
                     QLQQRRANQRLGAEVMSQRIREMSDNDPRVVALVIRQWINNDHE"
     gene            5981..6976
                     /gene="fliG"
                     /locus_tag="J1Y83_002299"
     CDS             5981..6976
                     /gene="fliG"
                     /locus_tag="J1Y83_002299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliG"
                     /protein_id="HBA7630445.1"
                     /translation="MSNLTGTDKSVILLMTIGEDRAAEVFKHLSQREVQTLSAAMANV
                     TQISNKQLTDVLAEFEQEAEQFAALNINANDYLRSVLVKALGEERAASLLEDILETRD
                     TASGIETLNFMEPQSAADLIRDEHPQIIATILVHLKRAQAADILALFDERLRHDVMLR
                     IATFGGVQPAALAELTEVLNGLLDGQNLKRSKMGGVRTAAEIINLMKTQQEEAVITAV
                     REFDGELAQKIIDEMFLFENLVDVDDRSIQRLLQEVDSESLLIALKGAEQPLREKFLR
                     NMSQRAADILRDDLANRGPVRLSQVENEQKAILLIVRRLAETGEMVIGSGEDTYV"
     gene            6969..7655
                     /gene="fliH"
                     /locus_tag="J1Y83_002300"
     CDS             6969..7655
                     /gene="fliH"
                     /locus_tag="J1Y83_002300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707825.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly protein FliH"
                     /protein_id="HBA7630446.1"
                     /translation="MSDNLPWKTWTPDDLAPPPAEFVPMVESEETIIEEAEPSLEQQL
                     AQLQMQAHEQGYQAGIAEGRQQGHEQGYQEGLAQGLEQGLAEAKAQQAPIHARMQQLV
                     SEFQTTLDALDSVIASRLMQMALEAARQVIGQTPTVDNSALIKQIQQLLQQEPLFSGK
                     PQLRVHPDDLQRVDDMLGATLSLHGWRLRGDPTLHPGGCKVSADEGDLDASVATRWQE
                     LCRLAAPGVV"
     gene            7655..9028
                     /gene="fliI"
                     /locus_tag="J1Y83_002301"
     CDS             7655..9028
                     /gene="fliI"
                     /locus_tag="J1Y83_002301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellum-specific ATP synthase FliI"
                     /protein_id="HBA7630447.1"
                     /translation="MTTRLTRWLTTLDNFEAKMAQLPAVRRYGRLTRATGLVLEATGL
                     QLPLGATCVIERQNGSETHEVESEVVGFNGQRLFLMPLEEVEGVLPGARVYAKNISAE
                     GLQSGKQLPLGPALLGRVLDGSGKPLDGLPFPDTTETGALITPPFNPLQRTPIEHVLD
                     TGVRPINALLTVGRGQRMGLFAGSGVGKSVLLGMMARYTRADVIVVGLIGERGREVKD
                     FIENILGAEGRARSVVIAAPADVSPLLRMQGAAYATRIAEDFRDRGQHVLLIMDSLTR
                     YAMAQREIALAIGEPPATKGYPPSVFAKLPALVERAGNGISGGGSITAFYTVLTEGDD
                     QQDPIADSARAILDGHIVLSRRLAEAGHYPAIDIEASISRAMTALISEQHYARVRTFK
                     QLLSSFQRNRDLVSVGAYAKGSDPMLDKAIALWPQLEGYLQQGIFERADWEASLQGLE
                     RIFPTVS"
     gene            9047..9490
                     /gene="fliJ"
                     /locus_tag="J1Y83_002302"
     CDS             9047..9490
                     /gene="fliJ"
                     /locus_tag="J1Y83_002302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis chaperone FliJ"
                     /protein_id="HBA7630448.1"
                     /translation="MAEHGALATLKDLAEKEVEDAARLLGEMRRGCQQAEEQLKMLID
                     YQNEYRNNLNSDMSAGMTSNRWINYQQFIQTLEKAITQHRQQLNQWTQKVDIALNSWR
                     EKKQRLQAWQTLQERQSTAALLAENRLDQKKMDEFAQRAAMRKPE"
     gene            9487..10614
                     /gene="fliK"
                     /locus_tag="J1Y83_002303"
     CDS             9487..10614
                     /gene="fliK"
                     /locus_tag="J1Y83_002303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook length control protein FliK"
                     /protein_id="HBA7630449.1"
                     /translation="MIRLAPLITADVDNATLPGGKASDAAQDFLALLSEALTGEATTD
                     KATPQLLVATDKPTAKGEPLVSDIVSDAQQADLLIPVDETPPVINDEQSTSTPLTTAQ
                     TITLAAAADNNTAKDEKADDLNEDVTASLSALFAMLPGFDNTPKVTDAPSTVLPTEKP
                     TLFTKLTSAQLTTAQADDAPGTPAHPLTPLVAEAQSKAEVISTPSPVTAAASPLITPH
                     QTQPLPTVAAPVLSAPLGSHEWQQSLSQHISLFTRQGQQSAELRLHPQDLGEVQISLK
                     VDDNQAQIQMVSPHQHVRAALEAALPVLRTQLAESGIQLGQSNISGESFSGQQQAASQ
                     QQQSQRTANHEPLAGEDDNTLPVPVSLQGRVTGNSGVDIFA"
     gene            10719..11183
                     /gene="fliL"
                     /locus_tag="J1Y83_002304"
     CDS             10719..11183
                     /gene="fliL"
                     /locus_tag="J1Y83_002304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body-associated protein FliL"
                     /protein_id="HBA7630450.1"
                     /translation="MTDYAISKKSKRSLWFPILVFITLAACASAGYSYWHSHQVAADD
                     KAQQRVVRSPVFYALDTFTVNLGDADRVLYIGITLRLKDEATRSRLSEYLPEVRSRLL
                     LLFSRQDAAVLATEDGKKNLIAEIKTTLSTPLVAGQPKQDVTDVLYTAFILR"
     gene            11188..12192
                     /gene="fliM"
                     /locus_tag="J1Y83_002305"
     CDS             11188..12192
                     /gene="fliM"
                     /locus_tag="J1Y83_002305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliM"
                     /protein_id="HBA7630451.1"
                     /translation="MGDSILSQAEIDALLNGDSEVKDEPTASVSGDSDIRPYDPNTQR
                     RVVRERLQALEIINERFARHFRMGLFNLLRRSPDITVGAIRIQPYHEFARNLPVPTNL
                     NLIHLKPLRGTGLVVFSPSLVFIAVDNLFGGDGRFPTKVEGREFTHTEQRVINRMLKL
                     ALEGYSDAWKAINPLEVEYVRSEMQVKFTNITTSPNDIVVNTPFHVEIGNLTGEFNIC
                     LPFSMIEPLRELLVNPPLENSRNEDQNWRDNLVRQVQHSQLELVANFADISLRLSQIL
                     KLKPGDVLPIEKTDRIIAHVDGVPVLTSQYGTLNGQYALRIEHLINPILNSLNEEQPK
                     "
     gene            12189..12602
                     /gene="fliN"
                     /locus_tag="J1Y83_002306"
     CDS             12189..12602
                     /gene="fliN"
                     /locus_tag="J1Y83_002306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliN"
                     /protein_id="HBA7630452.1"
                     /translation="MSDMNNPADDNNGAMDDLWAEALSEQKSTSGKSAADAVFQQFGG
                     GDVSGTLQDIDLIMDIPVKLTVELGRTRMTIKELLRLTQGSVVALDGLAGEPLDILIN
                     GYLIAQGEVVVVADKYGVRITDIITPSERMRRLSR"
     gene            12605..12970
                     /gene="fliO"
                     /locus_tag="J1Y83_002307"
     CDS             12605..12970
                     /gene="fliO"
                     /locus_tag="J1Y83_002307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system protein
                     FliO"
                     /protein_id="HBA7630453.1"
                     /translation="MNNHATVQSSAPVSAEPLLQVSGALIAIIALILAAAWLVKRLGF
                     APKRTGVNGLKISASASLGARERVVVVDVEDARLVLGVTAGQINLLHKLPPSAPTEEI
                     PQTDFQSVMKNLLKRGGRS"
     gene            12970..13707
                     /gene="fliP"
                     /locus_tag="J1Y83_002308"
     CDS             12970..13707
                     /gene="fliP"
                     /locus_tag="J1Y83_002308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310714.1"
                     /note="The bacterial flagellar biogenesis protein FliP
                     forms a type III secretion system (T3SS)-type pore
                     required for flagellar assembly; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system pore protein
                     FliP"
                     /protein_id="HBA7630454.1"
                     /translation="MRRLFSVAPVLLWLITPLAFAQLPGITSQPLPGGGQSWSLPVQT
                     LVFITSLTFIPAILLMMTSFTRIIIVFGLLRNALGTPSAPPNQVLLGLALFLTFFIMS
                     PVIDKIYVDAYQPFSEEKISMQEALEKGAQPLREFMLRQTREADLGLFARLANTGPLQ
                     GPEAVPMRILLPAYVTSELKTAFQIGFTIFIPFLIIDLVIASVLMALGMMMVPPATIA
                     LPFKLMLFVLVDGWQLLVGSLAQSFYS"
     gene            13717..13986
                     /gene="fliQ"
                     /locus_tag="J1Y83_002309"
     CDS             13717..13986
                     /gene="fliQ"
                     /locus_tag="J1Y83_002309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013365797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliQ"
                     /protein_id="HBA7630455.1"
                     /translation="MTPESVMMMGTEAMKVALALAAPLLLVALVTGLIISILQAATQI
                     NEMTLSFIPKIIAVFIAIIIAGPWMLNLLLDYVRTLFTNLPYIIG"
     gene            13995..14780
                     /gene="fliR"
                     /locus_tag="J1Y83_002310"
     CDS             13995..14780
                     /gene="fliR"
                     /locus_tag="J1Y83_002310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707835.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system protein
                     FliR"
                     /protein_id="HBA7630456.1"
                     /translation="MMQVTSDQWLSWLSLYFWPLLRVLALISTAPILSERSVPKRVKL
                     GLAMMITFAIAPSLPANDVPVFSFFALWLAVQQILIGIALGFTMQFAFAAVRTAGEII
                     GLQMGLSFATFVDPGSHLNMPVLARIMDMLALLLFLTFNGHLWLISLLVDTFHTLPIG
                     SEPLNSNAFLALIKAGSLIFLNGLMLALPLITLLLTLNLALGLLNRMAPQLSIFVIGF
                     PLTLTVGISLMAALMPLIAPFCEHLFSEIFNLLADIISELPLI"
     gene            15070..15693
                     /gene="rcsA"
                     /locus_tag="J1Y83_002311"
     CDS             15070..15693
                     /gene="rcsA"
                     /locus_tag="J1Y83_002311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator RcsA"
                     /protein_id="HBA7630457.1"
                     /translation="MSTIIMDLCSYTRLGLTGYLLSRGVKKREINDIETVDDLAIACD
                     SQRPSVVFINEDCFIHDASNSQRIKHIINQHPNTLFIVFMAIANVHFDEYLLVRKNLL
                     ISSKSIKPESLDDILGDILKKETTITSFLNMPTLSLSRTESSMLRMWMAGQGTIQISD
                     QMNIKAKTVSSHKGNIKRKIKTHNKQVIYHVVRLTDNVTNGIFVNMR"
     gene            complement(15737..15979)
                     /gene="dsrB"
                     /locus_tag="J1Y83_002312"
     CDS             complement(15737..15979)
                     /gene="dsrB"
                     /locus_tag="J1Y83_002312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein DsrB"
                     /protein_id="HBA7630458.1"
                     /translation="MPEKRNCSTLAQSHRRKTMKVNDRVTVKTDGGPRRPGVVLAVEE
                     FSEGTMYLVSLEDYPLGIWFFNEAGHQDGIFVEKAE"
     gene            16088..16315
                     /gene="yodD"
                     /locus_tag="J1Y83_002313"
     CDS             16088..16315
                     /gene="yodD"
                     /locus_tag="J1Y83_002313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416463.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide/acid resistance protein YodD"
                     /protein_id="HBA7630459.1"
                     /translation="MKTAKEYSDTAKREVSVDVDALLAAINEISESEVHRSQNDSEHV
                     SVDGREYHTWRELADAFELDIHDFSVSEVNR"
     gene            16611..17430
                     /locus_tag="J1Y83_002314"
                     /pseudo
     CDS             16611..17430
                     /locus_tag="J1Y83_002314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310720.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-3-phosphoglycerate phosphatase-related
                     protein"
     gene            complement(17427..19121)
                     /gene="yedQ"
                     /locus_tag="J1Y83_002315"
     CDS             complement(17427..19121)
                     /gene="yedQ"
                     /locus_tag="J1Y83_002315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis regulator YedQ"
                     /protein_id="HBA7630460.1"
                     /translation="MQHETKMENQSWLKKLARRLGPGHVVNLCFIVVLLFSTLLTWRE
                     VVVLEDAYISSQRNHLENVANALDKHLQYNVDKLIFLRNGMREALVAPLDFTSLRNAV
                     TEFEQHRDEHAWQIELNRRRTLPVNGVSDALVSEGNLLSRENESLDNEITAALEVGYL
                     LRLAHNSSSMVEQAVYVSRAGFYVSTLPTLFTHNVPTRYYGYVTQPWFIGHSQRENRH
                     RAVRWFTSQPEHASNTEPQVTVSVPVDSNNYWYGVLGMSIPVRTMQQFLRNAIDKNLD
                     GEYQLYDSKLRFLTSSNPDHPTGNIFDPRELALLAQAMEHDTRGGIRMDSRYVSWERL
                     DHFDGVLVRVHTLSEGVRGDFGSISIALTLLWALFTSMLLLSWYVIRRMVSNMYVLQS
                     SLQWQAWHDTLTRLYNRGALFEKACPLAKLCHTHQHPFSVIQVDLDHFKAINDRFGHQ
                     AGDRVLSHAAGLISSSLRAQDIAGRVGGEEFCVILPGANLTQAAEVAERIRLKLNEKE
                     MLIAKSTTIRISASLGVSSSEETGDYDFEQLQSLADRRLYLAKQAGRNRVFASDNA"
     gene            complement(19292..19474)
                     /locus_tag="J1Y83_002316"
     CDS             complement(19292..19474)
                     /locus_tag="J1Y83_002316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2158 domain-containing protein"
                     /protein_id="HBA7630461.1"
                     /translation="MSFMVSEEVTVKEGGPRMIVTGYSSGMVECRWYDGYGVKREAFH
                     ETDLVPGEGSRSAEEV"
     gene            complement(19553..20470)
                     /locus_tag="J1Y83_002317"
     CDS             complement(19553..20470)
                     /locus_tag="J1Y83_002317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF808 domain-containing protein"
                     /protein_id="HBA7630462.1"
                     /translation="MLLAGSSLLTLLDDIATLLDDISVMGKLAAKKTAGVLGDDLSLN
                     AQQVSGVRANRELPVVWGVAKGSLINKVILVPLALIISAFIPWAITPLLMIGGAFLCF
                     EGVEKVLHMLEARKHKEDPAQSQQRLEKLAAQDPLKFEKDKIKGAIRTDFILSAEIVA
                     ITLGIVAEAPLLNQVLVLSGIALVVTVGVYGLVGVIVKIDDLGYWLAEKSSALMQALG
                     KGLLIIAPWLMKALSIVGTLAMFLVGGGIVVHGIAPLHHAIEHIAGQQSAVVAMILPT
                     VLNLILGFIIGGIVVLGVKAVAKMRGQAH"
     gene            20643..21563
                     /gene="yedA"
                     /locus_tag="J1Y83_002318"
     CDS             20643..21563
                     /gene="yedA"
                     /locus_tag="J1Y83_002318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="drug/metabolite exporter YedA"
                     /protein_id="HBA7630463.1"
                     /translation="MRFRQLLPLFGALFALYIIWGSTYFVIRIGVESWPPLMMAGVRF
                     LAAGILLLAFLLLRGHKLPPLRPLLNAALIGLLLLAVGNGMVTVAEHQNVPSGIAAVV
                     VATVPLFTLCFSRLFGIKTRKLEWVGIAIGLTGIIMLNSGGNLSGNPWGAILILIGSI
                     SWAFGSVYGSRITLPVGMMAGAIEMLAAGVVLMIASMIAGEKLTALPSLSGFLAVGYL
                     ALFGSIIAINAYMYLIRNVSPALATSYAYVNPVVAVLLGTGLGGETLSKIEWLALGVI
                     VFAVVLVTLGKYLFPAKPVVAPVIQDASSE"
     gene            complement(21552..22022)
                     /locus_tag="J1Y83_002319"
     CDS             complement(21552..22022)
                     /locus_tag="J1Y83_002319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="very short patch repair endonuclease"
                     /protein_id="HBA7630464.1"
                     /translation="MVDVHDKATRSKNMRAIATRDTAIEKRLASLLTGQGLAFRVQDA
                     SLPGRPDFVVDEYRCVIFTHGCFWHHHHCYLFKVPATRTEFWLEKIGKNVERDRRDIS
                     RLQELGWRVLIVWECALRGREKLTDAALTERLEEWICGEGASAQIDTQGIHLLA"
     gene            complement(22003..23421)
                     /locus_tag="J1Y83_002320"
     CDS             complement(22003..23421)
                     /locus_tag="J1Y83_002320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HBA7630465.1"
                     /translation="MQENISVTDSYSTGNAAQAMLEKLLQIYDVKTLVAQLNGVGENH
                     WSAAILKRALANDSAWHRLSEKEFAHLQTLLPKPPEHHPHYAFRFIDLFAGIGGIRRG
                     FESIGGQCVFTSEWNKHAVRTYKANHYCDPATHHFNEDIRDITLSHQEGVSDEAAAEH
                     IRQHIPEHDVLLAGFPCQPFSLAGVSKKNSLGRAHGFACDTQGTLFFDVVRIIDARRP
                     AMFVLENVKNLKSHDKGKTFRIIMQTLDELGYDVADAADNGPDDPKIIDGKHFLPQHR
                     ERIVLVGFRRDLNLKADFTLRDISECFPAQRVTLAQLLDPMVEAKYILTPVLWKYLYR
                     YAKKHQARGNGFGYGMVYPNNPQSVTRTLSARYYKDGAEILIDRGWDMATGEKDFDDP
                     LNQQHRPRRLTPRECARLMGFEAPGEAKFRIPVSDTQAYRQFGNSVVVPVFAAVAKLL
                     EPKIKQAVALRQQEAQHGRRSR"
     gene            complement(23488..24183)
                     /locus_tag="J1Y83_002321"
     CDS             complement(23488..24183)
                     /locus_tag="J1Y83_002321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohydrolase"
                     /protein_id="HBA7630466.1"
                     /translation="MELQHWQAQFDNWLKNHHQHQDAAHDLCHFRRVWATAQKLAADD
                     DVDMLVILTACYFHDIVSLAKNHPQRQRSSILAAEETRRLLREEFAQFPAEKIEAVCH
                     AIAAHSFSAQIAPLTTEAKIVQDADRLEALGAIGLARVFAVSGALGLALFDGEDPFTQ
                     HRPLDDKRYALDHFQTKLLKLPQTMQTARGKQLAQHNAQFLVEFMAKLGAELAGENEG
                     IDHKVIDAFSPAG"
     gene            complement(24223..24588)
                     /locus_tag="J1Y83_002322"
     CDS             complement(24223..24588)
                     /locus_tag="J1Y83_002322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HBA7630467.1"
                     /translation="MEKCDFYHIIVLSLNFPGYLKMEYGSTKMEERLSRSPGGKLALW
                     AFYTWCGYFVWAMARYIWVMSRIPDAPVSGFESDLGSTAGKWLGALVGFLFMALVGAL
                     LGGIAWYTRPRPARCRRYE"
BASE COUNT         6158 a         6369 c         6169 g         6013 t
ORIGIN      
        1 aaagggctca cgatgaaaaa attaacagtg gcaatttctg ctgtagctgc atcagtactg
       61 atggcgatgt ctgctcaggc agcagagatt tacaataaag acagtaacaa gctggatctg
      121 tacggaaaag ttaatgctaa gcactacttc tcttctaacg atgcagatga tggcgacaca
      181 acttatgttc gtctgggctt caaaggcgaa actcaaatca acgatcaact gactggtttc
      241 ggtcagtggg aatatgaatt caaaggcaac cgtgctgaat ctcaaggttc ttccaaagat
      301 aaaacccgtc ttgccttcgc tggcctgaaa ttcggtgact acggctccat cgattatggc
      361 cgtaactacg gtgtagcata cgatatcggt gcatggaccg acgttctgcc agaattcggt
      421 ggcgatacct ggacccaaac agatgtgttc atgactggtc gcaccacagg tgttgcaacc
      481 tatcgtaaca acgacttctt tggtctggtt gatggtctga actttgctgc tcagtatcag
      541 ggtaaaaatg accgcactga cgtaactgaa gctaacggtg atggtttcgg tttctccact
      601 acttatgagt atgaaggatt cggtgtaggt gcaacctatg ctaaatctga ccgcactaat
      661 gatcaggtta tctacggtaa caacagcctg aatgcatctg gtcaaaatgc tgaagtatgg
      721 gcagctggtc tgaaatatga tgcgaacaac atctatctgg ctaccaccta ttctgaaacc
      781 cagaacatga ctgtttttgg taataaccat attgccaaca aagcacaaaa cttcgaagta
      841 gttgcacaat atcagttcga cttcggtctg cgtccgtccg ttgcttacct gcaatctaaa
      901 ggaaaagact tgggtgcgtg gggtgatcag gacctggttg aatatattga tgtaggtgca
      961 acctattact tcaacaaaaa tatgtccact tttgttgatt acaaaatcaa cctgattgat
     1021 aagagcgatt tcacgaaagc atctggcgtt gctaccgatg atatcgttgc tgtaggattg
     1081 gtttaccagt tctaatctga ttactcaaag atatgttgtg ggaggctttg cctccccaac
     1141 atataagtgg ccccctcaag ccactttcct tagaagcact atcttgcttc tgactatata
     1201 aaccttctgt tatatattac cctttatttt ctggggcgtt tcaacgcccc atttttaata
     1261 atacccagtg aacaattaac gtatcaacta gatttaataa caacgatatc cgtacctaac
     1321 ctgatatcta atgtcataag tatcccttta attatcccct caacctcttg taactttccc
     1381 actcttgagc tccacatagt ccaaacgccg gatgcaaaaa aatcacacgt gcatttctga
     1441 aattgtgatg gaggaagata gtatcaatta tatgtgctta taaacgtccc cgattagcgc
     1501 agaagtcacc ggattgttca ggatacaata ggttgagtat gccgtattta ttttccaaaa
     1561 taaaaatcga tgctgttgct ccttcataaa atacttttac tctctggacg cttttcttag
     1621 ctctcttggt gttatcttaa aacgattatg gaacctttca gtaaaacgag aaggacactt
     1681 gtaaccattt tccctggcaa tctcgctgat gggttttact gtcgtttgta tagcagacaa
     1741 cgcattattt aacctcacgt cgtccaatat actttgaaaa cttattccct cgcttgccag
     1801 acggcgatgc aatgtggaaa cagaaatgta gagatatctc gctaccttgt ttgctgtcca
     1861 ttttgttcca ggttcggata gcattaggtt ataacaacga cttattaatg attgtttact
     1921 atctgataaa agtaaacaat taacatgatg tgctcctaat gaaagtagaa cgcccattgc
     1981 taagtgttcc tgaatttgcg ttgagaagcc tcggaaaata gatgttttta gttgctccca
     2041 acaatatatt aactcggggt tctgaggtaa gcagaaacct gtcgtgttac gaatttgatc
     2101 agttatcgca taaaattttt gaaacttttc aattagatca atgggataat aaagcatttc
     2161 tgcaagataa agacctgctt ctggataatt cgcaatataa aattcatatc cacagggaaa
     2221 taatattatc tgattattat caacagttag actatgcgtc tcccaattga taactttttt
     2281 tccctgacgg atacgacata aagctggcat aagaggctta accctgtgaa tctcatgatg
     2341 tttatgcatc cgtatttctt caatctttag gttagactta tcttttgcca gcatactctt
     2401 accctacttt atctcataaa ctggtgttat ctcagcggtt gcaattttat tggcattaag
     2461 catataacca actaacgctc cgctggaatt agaatccaca ggaatcttat cagtttttag
     2521 agcccatact ttaaactggt aatgatgggg tttatctcct ttgggaggac atgctccacc
     2581 aaatcctgca taaccaaaat catttcggcc ttgaacagca ccagtcggaa gttttgtacc
     2641 atcacgtctt cctgcatcag tgggcaaata tgttacagtt gctggaatat tagcaacagt
     2701 ccagtgccac catccactgc ctgtaggagc atctggatca tacacagtta cggcaaagct
     2761 tttggtgcct tcaggagcac cagaccaggt taatgagggc gatgtattac ccccttcaca
     2821 cccaaatcca gaaaatacat gagacgttgt aagttgctct cctgttttta tttcattact
     2881 agtgacctga aatgctgcgg cctgcgcaga aaatgttatg aatgccaata cacttgaaac
     2941 gataagtttt ttcataaaaa cctcattgtt gtgacctatc gttattttat ttgatgtgcc
     3001 ttgatctcat tatgcataaa ggcgcaatga tcgcgcaaat gcaatcataa ttataaaggt
     3061 gccccgaaca ttagccataa tatgcacaaa tcagattcct gttatctcaa ggataattat
     3121 tttttaatgc ggtgcacttc gtgacaacaa gttaatgact aacacaccgg cacaaatcaa
     3181 catcatgcct atgatagctg gcaggtccag tcgttgacca aaaaatcccc aggaaagcaa
     3241 actaatcaga acgataccga ctcctgacca gatagcataa gcaatccctg taggtatata
     3301 agccagcgtc tgagctaata accagaatga tgcacaatag caaataattg tgccaacaga
     3361 gggccataac cgtgtaaacc cctctgaaaa cttcattaag gttgtaccaa tgacctctgc
     3421 aagtattgcg ccaccaagat aaatataagg gttcatagca tattctttcc tgttcatact
     3481 ggagagaatt gtactacagt ttgaacacaa ctcaccgttt tcatcctggt gtatgtgtat
     3541 ccagggggct ctggcgtggc aagagataaa ggcaggaaca ataatgatca gaaggaaccc
     3601 caggctaacg aatgagttca ctcttcctgg gagcaaagcg aagacaaaac atgttatcgc
     3661 gtttacggat cgtggatggc agaaaacaac caggatcagg tatccatttt aaaccagaaa
     3721 ttgagtgagt ttgccccatc catgccccac gctgtgggat cggatataat taaacaagca
     3781 tagtcaacag gttacacaac ctacacctgc atggacatca cttcctgata cgccgccacc
     3841 agcttattac gcacctgaat ccccatttgc atagaaactg aggctttttg catatcggtc
     3901 atcacatcgt ttagcgccac gccgggttca ccgagggtga atttttctgc ctgcgtgcgg
     3961 gcgactgttt gtgtatcgct tatgcgatcg agcgcggcgt gcagctgccc ggcaaaacta
     4021 atggtcggtt gcggcagtga ttcctgcgca cgcgcactca tcgccgtagc ctgtaactgg
     4081 ctgataaccc cttcaatccc ctgtatcgct gacattttcg tctcccggat aatttctggt
     4141 aacaaagcct accagtaagt caataagaca aaggcgctaa atagcaacaa aaaaacgggt
     4201 ttattggcgg atagaaaaaa acgaaagcac aaataatggg agcgtcaatt tttcgagttt
     4261 gctgacccgg gagtgagtct tgttccactt tgccaataac gccgtccata atcagccacg
     4321 aggtgcgcga tgaatgcgac tgcagcccag acaaaatctc ttgagtggct taatcgcctg
     4381 cgtgcgaatc cgaaaattcc attgattgtt accggttccg cggcagtggc ggtcatggtc
     4441 gcactgatcc tgtgggcgaa agcccccgac taccgcacat tattcagcaa tctttccgat
     4501 caggatggtg gagcaattgt cagccaactg acgcaaatga atattcctta ccgcttcagc
     4561 gaagccagcg gcgctattga ggttccggca gataaagttc acgaactgcg cctgcgcctg
     4621 gcgcaacaag gtttgcccaa aggcggcgcg gtcggtttcg aactgctcga tcaggaaaag
     4681 tttggtatca gccagttcag cgaacaggtg aattatcagc gggcgctgga aggcgagctt
     4741 tctcgtacca tcgaaactat cggcccggta aaaggggcgc gcgtacactt ggcaatgccg
     4801 aaaccgtctt tattcgtccg tgaacaaaaa tccccttctg catcggtgac ggtaaatctg
     4861 ttacccggcc gcgcactcga tgaagggcaa attagcgcta ttgtacatct ggtttccagc
     4921 gcggttgctg gtctgccgcc gggaaacgtc acgctggtgg atcagggcgg gcatctgtta
     4981 acccagtcca ataccagcgg gcgcgatctt aatgacgctc agttgaaata tgccagcgat
     5041 gtcgaaggcc gtattcagcg gcgtattgaa gcgatcctgt cgcctattgt tggtaacggt
     5101 aatattcacg cccaggtctc ggcgcaactg gacttcgcca gtaaagaaca aacggaagaa
     5161 cagtatcgcc ctaacggtga tgaatctcat gcggcgcttc gttcacgcca gcttaatgag
     5221 agcgagcaaa gcggttccgg ttatccgggc ggcgtaccgg gggcgttgtc gaatcaaccg
     5281 gcacctgcga ataacgcgcc aatcagcacg cctccgacaa atcaaaataa ccgccagcag
     5341 caggcgagca ccaccagcaa tagtgggccg cgtagcacac agcggaatga aaccagtaac
     5401 tacgaagtcg atcgcaccat tcgtcatacc aaaatgaacg tgggcgatgt gcaacgtctg
     5461 tcagtcgcgg tagtggtgaa ttacaaaacc ttgccagacg gtaaaccatt gcctctcagc
     5521 aacgaacaga tgaagcaaat tgaagctctg acccgcgagg cgatgggctt ttctgaaaaa
     5581 cgcggtgatt cgctcaatgt tgttaactcg ccgttcaata gcagtgacga aagcggcgga
     5641 gcactgccgt tctggcaaca gcaagtgttt atcgatcagt tgctggctgc cggtcgctgg
     5701 ttgctggtac tgctggttgc gtggttgctg tggcggaaag cggtacgccc gcagctaaca
     5761 cgtcgcgccg aggcggtgaa gactgtgcag caacaggcgc aggcccgcga ggaagtggaa
     5821 gatgcggtgg aagtccgtct gagcaaagac gaacaactcc aacaacggcg cgctaaccaa
     5881 cgactggggg cagaagtcat gagccagcgt atccgtgaaa tgtctgataa cgatccgcgc
     5941 gtggtggcgc tggtcattcg ccagtggata aataacgatc atgagtaacc tgacaggcac
     6001 cgataaaagc gtcatcctgc tgatgaccat tggcgaagac cgggcggcag aggtgttcaa
     6061 gcacctctcc cagcgcgaag tgcaaaccct gagcgctgca atggcgaacg tcacgcagat
     6121 ctccaacaag cagctaaccg atgtgctggc agagtttgag caagaagctg aacagtttgc
     6181 cgcactgaat atcaacgcca acgattatct gcgttcggta ttggtcaaag ctctgggtga
     6241 agaacgtgcc gccagcctgc tggaagatat tctcgaaacc cgcgataccg ccagcggtat
     6301 tgaaacgctc aactttatgg agccgcagag cgccgccgat ctgattcgcg atgagcatcc
     6361 gcaaattatc gccaccattc tggtgcatct gaagcgcgcc caggccgccg atattctggc
     6421 gttgttcgat gaacgtctgc gccacgacgt gatgttgcgt atcgccacct ttggcggcgt
     6481 gcagccagcc gcgctggcgg aactgaccga agtactgaat ggcttgctcg acggtcagaa
     6541 tctcaagcgc agcaaaatgg gcggcgtgag aacggcagcc gaaattatca acctgatgaa
     6601 aacgcagcag gaagaagccg ttattaccgc cgtgcgtgaa ttcgacggcg agctggcgca
     6661 gaaaatcatc gacgagatgt tcctgttcga gaatctggtg gatgtcgatg atcgcagcat
     6721 tcagcgtctg ttgcaggaag tggattccga atcgctgttg atcgcgctga aaggggccga
     6781 gcagccactg cgcgagaaat tcctgcgcaa tatgtcgcag cgtgccgccg atattctgcg
     6841 cgacgatctc gccaaccgtg gtccggtgcg tctgtcgcag gtcgaaaacg aacagaaagc
     6901 gattctgctg attgtgcgcc gtcttgccga aactggcgag atggtgattg gcagcggcga
     6961 ggatacctat gtctgataat ctgccgtgga aaacctggac gccggacgat ctcgcgccac
     7021 caccggcaga gtttgtgcca atggtcgagt cggaagaaac cattattgaa gaggccgaac
     7081 ccagccttga gcagcaactg gcgcaactgc aaatgcaggc ccacgagcag ggttatcagg
     7141 cggggattgc cgaaggtcgc cagcaaggtc atgaacaggg ctatcaggaa ggactggccc
     7201 aggggctgga acaaggtctg gcagaggcga aggctcaaca agcgccaatt catgcccgga
     7261 tgcagcaact ggtcagcgaa tttcaaacta cccttgatgc gcttgatagt gtgatagctt
     7321 cgcgcctgat gcagatggcg ctggaggcag cacgtcaggt cataggtcag acgccaacgg
     7381 tggataactc ggcactgatc aaacagatcc aacagttgtt gcagcaagaa ccattattca
     7441 gcgggaaacc acagctgcgc gtacacccgg atgatctgca acgtgtggat gatatgctcg
     7501 gcgcaacctt aagtttgcat ggctggcgct tgcggggcga tcccaccctc catcctggcg
     7561 gctgtaaagt ctccgccgat gaaggcgatc tcgacgccag tgtcgccacc cgctggcaag
     7621 aactctgccg tctggcagca ccaggagtgg tgtaatgacc acgcgcctga ctcgctggct
     7681 aaccacgctg gataactttg aagccaaaat ggcgcagttg cctgcggtac gtcgctatgg
     7741 gcgattaacc cgcgctaccg ggctggtgct ggaagccacc ggattacaat tgccgctcgg
     7801 cgcaacctgt gtcattgagc gccagaacgg cagcgaaacg cacgaagtag aaagcgaagt
     7861 cgttggcttt aacggtcaac ggctgttttt aatgccgctg gaggaagtgg aaggtgtcct
     7921 gcccggcgcg cgtgtatatg ccaaaaacat ttcggcagaa gggctgcaaa gcggcaagca
     7981 gttgccgctc ggtccggcgt tattaggtcg tgttctggac ggtagcggta aaccgctcga
     8041 tggcctgccc ttccccgata cgacggaaac cggtgcgctg attaccccgc catttaaccc
     8101 gttgcaacgt acaccgattg aacatgtgct ggacaccggc gtgcgcccaa tcaatgccct
     8161 gcttaccgtc ggacgcgggc agcgtatggg gctgtttgcc gggtccggcg ttggtaaaag
     8221 tgtgctgctg gggatgatgg cccgttacac ccgcgccgat gtcattgtcg tgggtttgat
     8281 tggtgaacga gggcgcgaag taaaagattt tattgagaat atcctcggtg ccgaagggcg
     8341 tgcacgctca gtggtgattg ccgctccggc ggatgtttct ccgctcctgc gaatgcaggg
     8401 tgccgcctac gccacgcgca ttgccgaaga ttttcgcgat cgtggtcagc atgtgttgct
     8461 gattatggac tccctcaccc gctacgcgat ggcccagcgt gaaattgcgc tggcgattgg
     8521 cgaaccacct gccactaaag gttatccacc gtcggtgttt gccaaattac cggcactggt
     8581 cgagcgtgcc ggtaatggca ttagcggcgg cggctcgatt accgcgtttt atacggtgct
     8641 caccgaaggc gatgaccagc aggaccccat tgccgactcc gcgcgggcga tcctcgacgg
     8701 tcacattgtg ctgtctcgcc gactggcgga agccgggcac tatccggcta tcgatattga
     8761 agcgtcgatc agccgcgcaa tgacggcgtt gatcagcgag caacattatg cgcgagtgcg
     8821 caccttcaaa cagctgttgt cgagttttca gcgtaaccgc gatctggtta gcgtcggcgc
     8881 gtatgccaaa ggcagcgatc cgatgctcga caaagccatc gccctgtggc cgcagctgga
     8941 gggctatttg caacaaggca tttttgaacg cgcggactgg gaagcgtctc tccaggggct
     9001 ggagcgtatt ttcccgacag tgtcataacc caggagataa cggcagatgg cggaacatgg
     9061 tgcgctggcg accctgaaag atctggcaga aaaagaggta gaggatgccg ctcgcctgct
     9121 gggtgaaatg cgtcgcggat gtcagcaggc ggaagaacaa ctcaaaatgt tgatcgatta
     9181 tcagaatgaa tatcgcaata acctcaatag cgatatgagt gccgggatga ccagcaaccg
     9241 ctggatcaac tatcagcagt ttatccagac gctggaaaaa gccattactc agcatcgcca
     9301 gcaacttaat cagtggacgc agaaagttga cattgccctg aacagctggc gagaaaagaa
     9361 acaacgtttg caggcctggc agacactgca ggaacggcaa tccacggcgg cactgcttgc
     9421 agaaaaccgc ctcgatcaga aaaagatgga tgagttcgcc cagcgcgccg ccatgaggaa
     9481 acctgaatga ttcgcttagc gcccttgatt accgccgacg ttgataacgc cacattgcct
     9541 ggcggcaaag ccagcgatgc tgcacaagat tttctcgcgt tgttgagcga agcattaaca
     9601 ggcgaggcaa caaccgacaa agcgactccc cagttgctgg tggcaacaga taagcccacg
     9661 gcaaaaggcg agccgctggt cagcgatatt gtttccgacg cgcaacaagc agatttactg
     9721 atccctgtgg atgaaacacc gcctgtcatc aacgacgaac aatccacatc aacaccgtta
     9781 accaccgctc agacgataac gttggctgcg gcggctgaca acaatacggc aaaagacgaa
     9841 aaagcggatg atctgaatga agacgtcacc gcaagcctga gtgccctttt tgcgatgttg
     9901 ccgggttttg acaatacgcc caaagtgact gatgcaccgt caaccgtatt accgacagag
     9961 aaaccaacgc tcttcacaaa actgacttct gcgcaactca caacagcaca ggctgatgac
    10021 gcccccggca caccagctca tccattaaca ccgctggtag cagaagccca gagtaaagcg
    10081 gaagtcatca gcacaccttc accggtgacc gctgccgcca gcccgctaat cactccacac
    10141 cagacacagc cactgcccac cgtcgccgcg cctgtcttga gtgcaccgct gggttctcac
    10201 gaatggcaac aatcattaag ccagcatatt tcgctgttca cccgccaggg gcaacaaagt
    10261 gcagagttgc gtctgcaccc gcaggattta ggtgaagtgc aaatttccct caaagtggat
    10321 gataaccagg cgcaaatcca gatggtttca ccgcatcagc atgtacgcgc cgccctggaa
    10381 gcagcgctgc cggtactgcg tacgcagctg gccgaaagtg gcattcagtt agggcaaagc
    10441 aacattagtg gcgaaagctt tagtggtcag cagcaggccg cttcccagca acagcaaagc
    10501 caacgcacag caaaccatga acctctggcg ggggaagacg acaatacgct tccggttccc
    10561 gtctctttac aagggcgtgt aacaggtaac agcggcgttg atattttcgc ctaacgtcag
    10621 aggtagcacc gtaatccgcg tcttttcccc gctttgttgc gctcaagacg caggataatt
    10681 agccgataag cagtagcgac acaggaagac cgcaacacat gactgattac gcgataagca
    10741 agaaaagcaa gcgatcgctt tggttcccga ttctggtatt cattaccctc gcggcctgtg
    10801 ccagcgcagg ttacagctac tggcattcgc atcaggttgc cgctgacgac aaagcgcagc
    10861 aacgcgtcgt gcgctccccg gtcttctatg cgctggatac ctttacggtc aatttgggcg
    10921 atgcggatcg cgtgctttat atcggcataa ccctgcgact gaaagatgaa gcaacccgct
    10981 cgcggctgag tgagtatttg ccggaagtcc gtagtcgctt gctgttactg ttttcgcgtc
    11041 aggatgctgc cgtactggcg acagaagatg gcaagaaaaa cctgattgcc gagattaaaa
    11101 ccacactttc caccccgctt gttgccgggc aaccgaaaca ggatgtcacc gacgtgctgt
    11161 ataccgcttt tattctgcga taacgacatg ggcgatagta ttctttctca agctgaaatt
    11221 gatgcgctgt tgaatggtga cagcgaagtc aaagacgaac cgacggccag tgttagcggc
    11281 gatagtgaca tccgtccgta cgatccgaat acccaacgac gggttgtgcg cgaacgtttg
    11341 caggcgctgg aaatcattaa tgagcgcttt gcccgccatt ttcgtatggg gctgttcaac
    11401 ctgctgcgcc gtagcccgga cattaccgtc ggggccatcc gcattcagcc gtaccatgaa
    11461 tttgcccgca acctgccggt gccgaccaac ctgaacctta tccaccttaa accgctgcgc
    11521 ggcacagggc tggtggtgtt ctcgccgagc ctggtgttta tcgccgtgga taacctgttt
    11581 ggcggcgatg gacgcttccc gaccaaagtg gaaggtcgcg agtttaccca tacagaacag
    11641 cgcgtcatca accgcatgtt aaagctggcg cttgaaggct atagcgatgc ctggaaggcg
    11701 attaatccgc tggaagttga gtacgtgcgt tcggaaatgc aggtgaaatt taccaatatc
    11761 accacctcac caaatgacat cgtggttaac acaccgttcc atgtggagat tggcaacctg
    11821 accggcgaat ttaatatctg cctgccattc agcatgatcg agccgctacg ggaattgttg
    11881 gttaacccgc cgctggaaaa ctcgcgtaat gaagatcaga actggcgcga taacctggtg
    11941 cgccaggtgc agcattcaca gctggaactg gtggccaact ttgccgatat ctcgctacgc
    12001 ctgtcgcaga ttttaaaact gaaacccggc gacgtcctgc cgatagaaaa aaccgatcgc
    12061 atcatcgccc atgttgacgg cgtcccggtt ctgaccagtc agtacggcac tctcaacggt
    12121 cagtatgcgt tacggataga acatttgatt aacccgattt taaattctct gaacgaggaa
    12181 cagcccaaat gagtgacatg aataatccgg ccgatgacaa caacggcgca atggacgatc
    12241 tgtgggctga agcgttgagc gagcaaaaat caaccagcgg aaaaagcgct gccgacgcgg
    12301 tgttccagca atttggcggt ggtgatgtca gcggaacgtt gcaggatatc gacctgatta
    12361 tggatattcc ggtcaagctg accgtcgagc tgggccgtac gcggatgacc atcaaagagc
    12421 tgttgcgtct gacgcaaggg tcagtcgtgg cgctggacgg tctggcgggc gaaccgctgg
    12481 atattctgat caacggttat ttaatcgccc agggcgaagt ggtggttgtt gccgataaat
    12541 atggcgtgcg gatcaccgat atcattaccc cgtccgagcg gatgcgccgc ctgagccgtt
    12601 agtgatgaat aaccatgcta ctgtgcaatc ttccgcgccg gtttctgctg agccactgct
    12661 gcaggtgagc ggcgcactca tcgccattat tgccctgatc ctcgctgctg cctggctggt
    12721 aaaacggctg ggatttgccc ctaaacgcac tggcgttaac ggtctgaaaa ttagcgccag
    12781 tgcttcgctg ggcgcgcgtg aacgggtcgt ggtggtcgat gtggaagatg cacggctggt
    12841 gctcggcgtt accgcagggc aaatcaatct gctgcataaa cttccccctt cagcaccaac
    12901 ggaagagata ccgcagaccg attttcagtc ggtcatgaaa aatttgctta agcgtggcgg
    12961 gagatcctga tgcgtcgttt gttttctgtc gcaccggtcc ttctctggct gattacgccc
    13021 ctcgccttcg cgcaactgcc gggtatcacc agccagccgc tacctggcgg tggacaaagc
    13081 tggtcgctcc cggtgcagac gctggtgttc atcacctcgt tgacgttcat tccagcgatt
    13141 ttactgatga tgaccagttt cacccgcatc attattgttt ttggtttatt gcgtaacgcg
    13201 ctgggaacgc cctccgcgcc acctaaccag gtattgctgg ggctggcact gtttttgacc
    13261 ttttttatta tgtcgccagt gatcgacaaa atttatgtcg atgcgtacca gccattcagc
    13321 gaagagaaaa tctcaatgca ggaggcgctg gaaaaagggg cgcagccgct gcgtgagttt
    13381 atgctgcgtc agacccgcga ggcagattta gggttgtttg ccagactggc gaataccggc
    13441 ccgttgcagg ggcctgaagc tgtgccgatg cgcattttgc tcccggccta cgtgaccagc
    13501 gagttgaaaa ccgcatttca gataggcttc acgattttca tccctttttt gattatcgac
    13561 ctggtgattg ccagcgtgtt gatggcattg gggatgatga tggttccccc cgccaccatt
    13621 gctctgccct ttaaactgat gctgtttgta ctggtggatg gctggcaatt gctggtcggt
    13681 tcgctggcgc agagctttta cagctagaga ggcaaaatga cacctgaatc ggtcatgatg
    13741 atgggaactg aagcgatgaa agtcgcgctg gcactggcag ccccgctgtt gctggtggcg
    13801 ctggtcacgg gccttatcat cagtattttg caggcagcca cgcagattaa cgaaatgacg
    13861 ctgtcgttta ttccgaaaat catcgccgta tttatcgcca ttattattgc cggaccgtgg
    13921 atgctcaatc tgttgctgga ttacgtccgc accttgttca ctaacctgcc gtatatcatc
    13981 gggtagcccg tacaatgatg caggtgacaa gcgatcaatg gctttcctgg ttaagcctgt
    14041 acttctggcc gttactgcgc gtgctggcgc tcatttctac cgcgccaatt ctgagcgaac
    14101 gcagcgtgcc gaaacgggta aaactgggtc tggcaatgat gatcacgttc gccattgccc
    14161 catcattacc tgccaacgat gttcctgttt tttcgttctt tgctctgtgg ctggccgtgc
    14221 agcagatcct gatcggcatt gcgcttggtt ttaccatgca atttgccttt gccgctgtgc
    14281 gaaccgctgg cgaaattatc ggtctgcaaa tggggctgtc atttgcgaca tttgtcgatc
    14341 ctggcagcca tctcaatatg cccgttttag cgcgcatcat ggatatgctg gcattactgc
    14401 tgttcctgac atttaacggt catttatggt tgatttcact gctggtcgat acctttcaca
    14461 ccctgccgat tggtagcgaa ccgttgaaca gcaatgcgtt tctggcactc atcaaagcag
    14521 ggagtttgat tttccttaac gggctgatgc tggcgttacc gctcattact ctgttgctga
    14581 ccctgaatct ggcattaggt ttactgaatc gtatggcccc gcaattatcc atttttgtta
    14641 ttggatttcc attaactctg actgtcggca tctctttaat ggcggcatta atgccgttaa
    14701 ttgcgccttt ttgcgaacat ttattcagtg aaatttttaa tttgctggct gatattatta
    14761 gtgaattacc attaatataa ttccgtaacg tttatcatgc taccctaagg attatccgaa
    14821 aaataatatc tgcgaacatc ttccaggata ctcctgtagc gaaatatttg ttttaagctc
    14881 actcacatat cgcaacattt actttacttt aagacaattc caggcaaatt atacaacact
    14941 ttacgggata gtaagtccgc ctgaaaaatt gcgagagtgg cgcattaggt gacccatgtt
    15001 gttccgttta gtcatgatga aatattcagg taaggggaat tatcgttacg cattgagtga
    15061 gggtatgcca tgtcaacgat tattatggat ttatgtagtt acacccgact aggtttaacc
    15121 gggtatctgt tgagtagagg ggttaaaaaa agagaaatca acgacattga aaccgttgat
    15181 gaccttgcca tagcttgtga ttcacagcgc ccttcagtgg tgtttattaa tgaggactgt
    15241 ttcatccacg atgcttctaa cagtcagcgc atcaagcaca tcattaatca acatcccaat
    15301 acgttattta tcgtttttat ggcaattgcc aatgttcatt ttgatgaata tctattggtc
    15361 agaaaaaatt tattgatcag ttctaaatcg attaaaccgg aatctctcga cgatatcctt
    15421 ggcgatattc tgaaaaaaga gacaacgata acctcgtttt taaatatgcc gacgttatca
    15481 ttgagccgaa ccgaatcgag tatgttgcga atgtggatgg caggtcaggg aaccattcaa
    15541 atctccgacc aaatgaatat caaagccaag accgtttcat cgcataaagg taatattaaa
    15601 cgtaagatca aaacgcataa taaacaggtt atctaccatg tcgtccgact gacggataat
    15661 gtgactaatg gtatttttgt caacatgcgc taacacattc tgactggtgg tttcccacca
    15721 gtcaggctga ataagattac tctgctttct caacaaagat accgtcctga tgccctgctt
    15781 cattaaagaa ccagatgccg agcgggtagt cttccagcga aaccaggtac attgtgcctt
    15841 cactaaactc ctcaactgcc agtaccacgc caggacgacg cggaccgcca tccgttttga
    15901 ctgttacccg atcattcacc ttcatcgttt tcctcctgtg gctttgtgcc agtgtagaac
    15961 aatttcgttt ttctggcagc gccaggcgcg cgcgagtgct gattttctcg acggtctata
    16021 cttaagagat gccaggcgga cttaacgact ggcggcaaca acagagtaac ggttgcgagg
    16081 aaagatgatg aaaaccgcaa aagagtacag cgataccgca aaacgtgagg tcagcgtcga
    16141 tgtcgatgcc ctgctggcgg cgatcaatga aattagcgaa agcgaagttc atcgcagcca
    16201 gaacgattct gaacacgtta gcgtcgatgg acgtgaatat catacatggc gtgaattggc
    16261 ggatgccttc gaactggata ttcatgactt cagcgtctct gaagtgaatc gttgaatgca
    16321 caataaaaaa atcccgaccc tgagggggtc gggatgaaac ttgcttaagc aagaagcact
    16381 taaaaaattc gttacaccag gaaatctgat gtgttcatca ccttatctgc aatttttttc
    16441 gctgacaaga aaatattcgc gctatgaatg attaacgcta cttatgaata gaaatgtgac
    16501 gcgcgtcacc ccttggtggc tctggtgtca acgggatcgc tcccgaaaaa atcatctttg
    16561 attatttgtg ctgttttgag aatcatcctg gtaaggggag tactgcgccc atgttttcaa
    16621 ttcaacaacc actactggtt tttagcgatc ttgatggcac cctgctggac agtcatagtt
    16681 atgactggca accggcagcc ccctggctca gccgtttaca cgaagccaat attcccgtca
    16741 ttctctgtag cagtaaaact tcagcggaaa tgctgtactt gcaaaaaaaa aatgttgggg
    16801 ctacaaggct taccgctgat tgcagagaat ggcgcagtga tccagcttgc tgaacaatgg
    16861 caggatatag acggttttcc acgcatcatc tcaggtatta gccatggcga aatctgccag
    16921 gttttaaata cgctacgcga gaaagaacat tttaaattca cgacttttga tgatgtcgac
    16981 gatgcaacca ttgccgaatg gacgggatta agccgtagcc aggcggcgct gacgcagctg
    17041 catgaggcgt cggtaacgct aatctggcgc gacagtgacg agcgtatggc acaatttatc
    17101 gctcgtctga acgaactggg cttgcagttt atgcagggtg cgcgcttctg gcacgtactg
    17161 gatgcctctg ccggaaaaga tcaggctgcc aactggatta tcgcgaccta tcaacaattg
    17221 tcaggcagac gcccaaccac acttggcctg ggcgatgggc caaacgatgc gcctttactg
    17281 gaggtaatgg attacgcggt gattgtgaaa gggctaaacc gtgaaggggt ccatctgcat
    17341 gatgaggatc cggcccgcgt ctggcgaacg cagcgtgaag gaccggaagg ctggcgtgaa
    17401 gggctggacc attttttccc cgcccgttaa gcgttatcgc tcgcgaatac ccgattgcgc
    17461 ccggcctgtt ttgcgaggta aagccgacgg tcggccaatg actggagttg ttcaaaatca
    17521 taatcaccgg tttcctcgct gctgcttacc cccagcgagg cactaatgcg tatcgttgta
    17581 ctcttggcga tcagcatctc tttttcattt aacttcaggc gaatacgttc tgcgacctcc
    17641 gcagcctgcg tcagattcgc gcctggcaga atcacacaaa actcctcacc accgacccgc
    17701 ccggcaatgt cctgcgcacg caaggaactg ctaattaatc cggcagcatg agaaagaaca
    17761 cggtcgcccg cctgatgacc aaagcggtca ttaatcgctt taaaatggtc aagatcgacc
    17821 tgaatgacag aaaaaggatg ttggtgcgtg tgacacaatt tagcgagcgg acaggctttt
    17881 tcgaacaatg cgccacggtt atataaacgc gttaaggtgt cgtgccacgc ctgccactgc
    17941 aacgagcttt gcagaacata catgttgctg accatccggc gaatcacata ccaggagagg
    18001 agtaacatgc tggtaaagag cgcccacagc agggttaatg caatgctgat actgccgaaa
    18061 tcgccgcgca cgccttcgct tagcgtatgg acgcgcacca gcacaccgtc gaaatggtcc
    18121 agacgttccc agctaacata gcgactgtcc atacgaatgc cgccccgcgt gtcatgttcc
    18181 attgcctgcg ccagcaaggc cagttcacga ggatcaaaaa tattccctgt tggatggtca
    18241 ggatttgaag aggtcaaaaa tctcagctta ctgtcataga gctgatactc accatcgagg
    18301 tttttatcga tggcgtttct taaaaattgc tgcatagtac gcacgggaat actcatcccc
    18361 agcacgccat accagtagtt attactgtct accggaacac tgacggtaac ctgcggttca
    18421 gtattgctgg cgtgttccgg ttgcgaagtg aaccagcgta ccgcgcggtg acgattttct
    18481 cgttgtgaat ggccgataaa ccagggttgg gtgacatagc cgtaataacg cgttggtaca
    18541 ttgtgcgtaa acaaggtcgg cagcgtcgaa acgtaaaatc cagcacgcga gacatacacc
    18601 gcctgttcaa ccatcgacga ggagttgtgc gccagtcgca gcaagtaacc aacttccagt
    18661 gcagcggtaa tttcattgtc gaggctttca ttttcgcgag acaggagatt cccctcgctg
    18721 actaatgcat ccgatacgcc attgactggc agggtgcgcc gtcggttgag ttcgatttgc
    18781 caggcgtgct cgtcgcgatg ctgttcgaac tcggttacgg cattacgcag agaggtgaaa
    18841 tccagtggcg ccacgagagc ttcgcgcatg ccattacgca aaaagatcag tttgtcgacg
    18901 ttatactgca aatgcttatc gagcgcgttg gctacgtttt ccagatgatt acgctggctg
    18961 gagatatagg catcttccag caccaccact tcacgccagg tgagcaaggt ggaaaaaagc
    19021 aataccacga taaagcagag attaacgaca tgaccgggcc ccaggcggcg tgcgagtttt
    19081 ttcaaccagc tctggttttc catttttgtc tcgtgctgca ccctgacgac tcccaacctg
    19141 cttttttatg attctggcat aacccggcga tagcgtaccg caaaacagcg ataattttat
    19201 aatggtggca tgataagacg cgactatcat tcattgcagg cattgggaca ccaggccgga
    19261 tgtgatccgg cctggtgtta atgataccct ttcaaacttc ttccgcagaa cgactcccct
    19321 cccccggaac aagatcggtt tcatgaaaag cttcccgctt gaccccgtaa ccgtcatacc
    19381 aacgacactc aaccataccg ctggagtatc cagtgacaat catccgcggg ccgccctctt
    19441 taaccgtaac ttcctcacta accataaagc tcatactcgc ctcctttttt cgagtgaaac
    19501 ttgttcacct tagttgaaga tggcgaattt tgcataatag ccagtgcgag tattaatgtg
    19561 cctgaccgcg cattttcgct actgctttca ctcccagcac cacgatgccg ccgatgataa
    19621 atccaagaat cagatttaaa acagtcggta atatcatcgc caccactgca ctttgctgcc
    19681 cggcgatatg ttcaatagcg tgatgcagcg gcgcaatacc atgtaccaca atcccgccgc
    19741 cgacgaggaa catcgccagc gtgccgacaa tcgataacgc cttcatcaac cagggcgcaa
    19801 tgatcaataa ccctttacct aatgcctgca tcagcgcgct ggatttttcc gccaaccaat
    19861 accccaggtc atcaatctta acgataactc ctaccagacc gtagacgccc acggtcacta
    19921 ccagcgcgat gcctgaaagc accagcacct gattaagcaa cggcgcttct gccacaatcc
    19981 ccagcgtgat ggcgacgatt tccgcagaca atataaaatc ggtacgaatc gcccctttta
    20041 ttttgtcttt ttcaaacttc agcggatcct gtgccgccag cttctccaga cgctgctgac
    20101 tctgcgccgg atcttcttta tgtttacgcg cctccagcat atgcagcact ttctctaccc
    20161 cttcaaagca gagaaacgcg ccgccaatca tcaacagtgg cgtaatcgcc cacgggataa
    20221 acgcactgat gatcagcgcc agcggcacca gaatcacttt attgatcagc gatcctttcg
    20281 ccacgcccca gaccacagga agttcccggt tggcccgcac gcctgaaact tgttgcgcat
    20341 tgagcgataa gtcatcccct aatacaccgg cggttttctt cgccgccagt ttgcccatca
    20401 cggagatatc gtccagcagt gtggcgatat catctagcaa cgtcagtaaa ctactcccgg
    20461 ccaaaagcat tccctctcat ttttattgtt gaagggtgta agtatgaagc aaaaacggca
    20521 gctctgatac ttgcttcaca ggtcaacatt tatttaacaa tcaagtgcaa ttatatctct
    20581 cgccagcaga atgattttca cgtttactat gaaccacctt tttatttcag ccgtgaggga
    20641 ttatgcgttt ccgccagttg ttaccgcttt ttggcgcgct gtttgcgttg tatatcattt
    20701 ggggctcaac ctattttgtc attcggattg gcgtggaaag ctggccgccg ttaatgatgg
    20761 cgggcgtgcg attcctggca gccggtattt tattgctggc atttttgcta ctgcgcggac
    20821 acaaacttcc cccgctacgt ccgctgctca atgccgcgct gattggcctg ttattgctgg
    20881 ctgtcggtaa tggcatggtg acggttgccg aacatcaaaa tgttccttcc ggcatcgccg
    20941 ccgtagtggt tgcaaccgtg cccctcttta ccctgtgctt cagccgcctg tttggcatta
    21001 aaacgcgcaa actggaatgg gtgggtatcg ccattgggct taccggaatc atcatgctca
    21061 atagcggtgg aaatttaagc ggcaatccgt ggggcgcgat tctgatttta atcggctcga
    21121 ttagctgggc gtttggctca gtttatggct cgcgcattac cttacctgta gggatgatgg
    21181 cgggtgcgat tgagatgctg gcggcaggcg tggtgttaat gatcgcatcg atgattgcgg
    21241 gtgaaaaact gacggcgctc ccttccctgt caggcttcct tgcggtcggc tatctggcgc
    21301 tgtttggttc gattatcgcc attaacgctt atatgtattt aatccgtaat gtcagtccgg
    21361 ctctcgccac cagctacgcc tacgttaacc cggtggtcgc ggtcttgctg ggtacgggac
    21421 tgggtggcga aacgctgtcg aaaattgaat ggctggcgct cggcgtaatt gttttcgcgg
    21481 tggtactggt cacgttggga aaatatctct tcccggccaa acccgttgtc gcgccagtta
    21541 ttcaggatgc atcaagcgag taaatgaatc ccctgcgtgt cgatctgcgc gctggcacct
    21601 tcgccgcaga tccactcttc cagacgctcg gtaagcgccg catccgtcag cttctcgcgc
    21661 ccacgtaacg cacactccca gacaatcagt acgcgccagc cgagctcctg caagcgactg
    21721 atatcgcggc gatcgcgttc aacattttta cctatcttct ccagccagaa ctcggttcgg
    21781 gtcgcaggca ctttaaacag atagcagtga tgatgatgcc agaagcagcc gtgggtaaat
    21841 atcacgcagc gatattcatc aacgacaaaa tccgggcgtc cgggcagact ggcgtcctga
    21901 acgcgaaacg caaggccctg cccggttaac aaactggcga ggcgcttctc aatcgccgta
    21961 tcacgcgtgg caatcgcgcg catatttttg ctgcgggtgg ccttatcgtg aacgtcgacc
    22021 atgttgtgcc tcttgctgac gcaacgccac cgcctgtttg atttttggct caagcagttt
    22081 tgccaccgcg gcaaagaccg gcacgaccac cgagttaccg aactggcgat aggcctgagt
    22141 gtccgaaacc ggaatgcgga atttcgcttc tcccggcgct tcaaaaccca ttaagcgcgc
    22201 gcattcacga ggtgttaacc gacgtggacg atgttgctga ttcagcggat cgtcaaagtc
    22261 tttctcaccc gtggccatat cccagccgcg gtcgattaaa atttccgcgc catctttgta
    22321 ataacgagca gacagcgtac gggtgacgct ttgcggattg ttcggataaa ccattccata
    22381 accgaagccg ttaccgcgcg cctgatgttt tttcgcatat cgatagaggt acttccacag
    22441 caccggcgtc aggatatatt tcgcctcaac catcgggtcc agcagctgcg ccagcgtcac
    22501 tcgctgtgca gggaaacatt cgctgatatc acgcagagta aaatcggctt tcagattcag
    22561 atcgcgacga aaacccacca gcacgatgcg ttcacggtgc tgcggcagga aatgtttacc
    22621 gtcgatgatt ttcggatcgt ccggcccgtt atctgctgcg tcggcaacgt catagcccag
    22681 ttcgtccagc gtctgcatga tgatgcggaa cgtttttccc ttatcgtgac tcttcaggtt
    22741 tttgacattt tcgagcacaa acatcgccgg acgacgcgcg tcgataatgc gcaccacatc
    22801 aaagaacagc gtaccctggg tatcgcaggc aaaaccgtgc gcccgcccga gcgagttctt
    22861 tttcgatacg ccagccagcg aaaatggctg acaagggaaa ccggccagta aaacatcgtg
    22921 ttcaggaatg tgttgacgaa tatgttccgc cgccgcctca tcactcacgc cttcttgatg
    22981 actgagggtg atgtcgcgga tatcttcatt aaaatgatgc gtcgccgggt cgcaataatg
    23041 gttggcttta taagtgcgta cagcatgttt gttccattcg ctggtaaaca cgcactgtcc
    23101 gccaatcgat tcaaaaccgc gacggatgcc gccaattccg gcaaatagat cgataaagcg
    23161 aaacgcataa tgcggatgat gttccggtgg tttgggtaac aacgtttgca gatgggcaaa
    23221 ctctttctca cttaaacggt gccatgccga gtcattcgcc agcgcacgtt ttaaaattgc
    23281 cgcgctccag tgattctcac ccacaccatt aagctgcgcc accagcgttt taacatcata
    23341 aatttgcagc agtttctcca gcattgcctg tgcggcattc ccggtgctgt atgaatcggt
    23401 tactgatata ttttcctgca tagatttaac cggccatctg agatggccga caggttaaca
    23461 taattactgc cgtttagggc cacgcgctca gccagcgggt gaaaacgcat ctatcacctt
    23521 gtgatcgata ccttcattct cccccgccag ttcggcaccg agcttcgcca taaactcgac
    23581 taaaaattgc gcgttgtgct gtgccagctg cttgcctctt gcggtttgca tggtttgcgg
    23641 taatttcagt aactttgtct ggaaatgatc cagcgcgtag cgtttatcat caagcgggcg
    23701 atgctgtgtg aacggatctt cgccatcaaa cagcgccaag cccaatgccc ctgaaacggc
    23761 aaatacgcgc gccaggccaa tcgcccccag ggcttccagc cgatccgcat cctggacgat
    23821 tttagcttcc gttgttaagg gggcgatttg cgcgctgaaa ctgtgagcgg caatggcatg
    23881 acaaacggcc tcaattttct ccgccggaaa ttgcgcgaac tcttcacgga gcaggcgacg
    23941 cgtctcttct gccgccagga ttgaagaacg ctgccgttgc ggatgatttt tcgccaggct
    24001 cacgatatcg tgaaaataac aggcggttaa aatcaccagc atatcgacat cgtcatctgc
    24061 cgccagtttc tgtgccgtag cccagacacg gcgaaaatga cacaagtcat gcgccgcgtc
    24121 ctgatgttga tgatggtttt tcaaccagtt atcgaactgt gcctgccagt gttgtaactc
    24181 catacgcgct ccgttgccaa agagtcgcaa ccttagcagt ttttattcat agcgtctgca
    24241 acgtgcggga cgaggtcggg tgtaccatgc aatgccgcct aacaaggcac caaccagggc
    24301 cataaacaga aatccgacca atgctcctaa ccacttcccc gcggtagatc ctaaatcact
    24361 ttcaaatccc gacacggggg catcggggat ccggctcatc acccagatgt atcgcgccat
    24421 tgcccagacg aaatagccac accatgtata gaaagcccac aaagccaatt tgccgccagg
    24481 gctgcgagag agtctctctt ccatctttgt cgaaccgtat tccattttga gataacccgg
    24541 aaaattcaga cttagaacta ttatgtgata aaagtcacat ttttccacat tggatgagcc
    24601 agtcaagact caatcatttt ttgaacaaat agataaattt tgcgcaacgg cggtgaatac
    24661 aggaaaaatc gatttgaacg atgttattta tctttgattt cggctttat
//
LOCUS       DADSYM010000068        24162 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2415
            SAMN08039579-rid11723173.denovo.068, whole genome shotgun sequence.
ACCESSION   DADSYM010000068 DADSYM010000000
VERSION     DADSYM010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039579
            Sequence Read Archive: SRR6362533
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 24162)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24162)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980162.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 03:39:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,484
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 4,347
            CDSs (with protein)               :: 4,347
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 43 of 85
            Pseudo Genes (incomplete)         :: 37 of 85
            Pseudo Genes (internal stop)      :: 19 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24162
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039579-rid11723173.denovo.068"
                     /strain="LGE2415"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8302 N 95.9628 W"
                     /collection_date="2015-08-24"
     gene            176..2419
                     /gene="pdeF"
                     /locus_tag="J1Y83_002323"
     CDS             176..2419
                     /gene="pdeF"
                     /locus_tag="J1Y83_002323"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase
                     PdeF"
                     /protein_id="HBA7630468.1"
                     /translation="MKLNATYIKIRDKWWGLPLFLPSLILPIFAHINTFAHISSGEVF
                     LFYLPLALMISMMMFFSWAALPGIALGIFVRKYAELGFYETLSLTANFIIIIILCWGG
                     YRVFTPRRNNVSHGDSRLISQRLFWQIVFPATLFLILFQFAAFVGLLASRENLVGVMP
                     FNLGTLINYQALLVGNLIGVPLCYFIIRVVRNPFYLRSYYSQLKQQVDAKVTKKEFAI
                     WLLALGALLLLLCMPLNEKSTIFSTNYTLSLLLPLMMWGAMRYGYKLISLLWAVVLMI
                     SIHSYQNYIPIYPGYTTQLTITSSSYLVFSFIVNYMAVLATRQRAVVRRIQRLAYVDP
                     VVHLPNVRALNRALRDAPWSALCYLRIPGMEMLVKNYGIMLRIQYKQKLSHWLSPLLE
                     PGEDVYQLSGNDLALRLNTESHQERITALDSHLKQFRFFWDGMPMQPQIGVSYCYVRS
                     PVNHIYLLLGELNTVAELSIVTNAPENMQRRGAMYLQRELKDKVAMMNRLQRALEHNH
                     FFLMAQPITGMRGDVYHEILLRMKGENDELISPDSFLPVAHEFGLSSSIDMWVIEHTL
                     QFMAENRAKMPAHRFAINLSPTSVCQARFPVEVSQLLAKYQIEAWQLIFEVTESNALT
                     NVKQAQITLQHLQELGCQIAIDDFGTGYASYARLKNVNADLLKIDGSFIRNIVSNSLD
                     YQIVASICHLARMKKMRVVAEYVENEEIREAVLSLGIDYMQGYLIGKPQPLIDTLNEI
                     EPIRESA"
     gene            complement(2458..3999)
                     /gene="ppx"
                     /locus_tag="J1Y83_002324"
     CDS             complement(2458..3999)
                     /gene="ppx"
                     /locus_tag="J1Y83_002324"
                     /EC_number="3.6.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exopolyphosphatase"
                     /protein_id="HBA7630469.1"
                     /translation="MPIHDKSPRPQEFAAVDLGSNSFHMVIARVVDGAMQIIGRLKQR
                     VHLADGLGPDNMLSEEAMTRGLNCLSLFAERLQGFSPASVCIVGTHTLRQALNATDFL
                     KRAEKVIPYPIEIISGNEEARLIFMGVEHTQPEKGRKLVIDIGGGSTELVIGENFEPI
                     LVESRRMGCVSFAQLYFPGGVINKENFQRARMAAAQKLETLTWQFRIQGWNVAMGASG
                     TIKAAHEVLMEMGEKDGIITPERLEKLVKEVLRHRNFASLSLPGLSEERKTVFVPGLA
                     ILCGVFDALAIRELRLSDGALREGVLYEMEGRFRHQDVRSRTASSLANQYHIDSEQAR
                     RVLDTTMQMYEQWREQQPKLAHPQLEALLRWAAMLHEVGLNINHSGLHRHSAYILQNS
                     DLPGFNQEQQLMMATLVRYHRKAIKLDDLPRFTLFKKKQFLPLIQLLRLGVLLNNQRQ
                     ATTTPPTLTLITDDSHWTLRFPHDWFSQNALVLLDLEKEQEYWEGVAGWRLKIEEEST
                     PEIAA"
     gene            complement(4004..6070)
                     /gene="ppk1"
                     /locus_tag="J1Y83_002325"
     CDS             complement(4004..6070)
                     /gene="ppk1"
                     /locus_tag="J1Y83_002325"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HBA7630470.1"
                     /translation="MGQEKLYIEKELSWLSFNERVLQEAADKSNPLIERMRFLGIYSN
                     NLDEFYKVRFAELKRRIIISEEQGSNSHSRHLLGKIQSRVLKADQEFDGLYNELLLEM
                     ARNQIFLINERQLSVNQQIWLRHYFKQYLRQHITPILINPDTDLVQFLKDDYTYLAVE
                     IIRGDTIRYALLEIPSDKVPRFVNLPPEAPRRRKPMILLDNILRYCLDDIFKGFFDYD
                     ALNAYSMKMTRDAEYDLVHEMEASLMELMSSSLKQRLTAEPVRFVYQRDMPNALVEVL
                     REKLTISRYDSIVPGGRYHNFKDFINFPNVGKANLVNKPLPRLRHIWFDKAQFRNGFD
                     AIRERDVLLYYPYHTFEHVLELLRQASFDPSVLAIKINIYRVAKDSRIIDSMIHAAHN
                     GKKVTVVVELQARFDEEANIHWAKRLTEAGVHVIFSAPGLKIHAKLFLISRKENGEVV
                     RYAHIGTGNFNEKTARLYTDYSLLTADARITNEVRRVFNFIENPYRPVTFDYLMVSPQ
                     NSRRLLYEMVDREIANAQQGLPSGITLKLNNLVDKGLVDRLYAASSSGVPVNLLVRGM
                     CSLIPNLEGISDNIRAISIVDRYLEHDRVYIFENGGDKKVYLSSADWMTRNIDYRIEV
                     ATPLLDPRLKQRVLDIIDILFSDTVKARYIDKELSNRYVPRGNRRKVRAQLAIYDYIK
                     SLEQSE"
     gene            complement(6242..6880)
                     /gene="purN"
                     /locus_tag="J1Y83_002326"
     CDS             complement(6242..6880)
                     /gene="purN"
                     /locus_tag="J1Y83_002326"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HBA7630471.1"
                     /translation="MNIVVLISGNGSNLQAIIDACKTNKIKGTVRAVFSNKADAFGLE
                     RARQAGIATHTLITSAFDSREAYDRELIHEIDMYAPDVVVLAGFMRILSPAFVSHYAG
                     RLLNIHPSLLPKYPGLHTHRQALENGDEEHGTSVHFVTDELDGGPVILQAKVPVFAGD
                     TEDDITARVQTQEHAIYPLVISWFADGRLKMHENAAWLDGQRLPPQGYAADE"
     gene            complement(6880..7917)
                     /gene="purM"
                     /locus_tag="J1Y83_002327"
     CDS             complement(6880..7917)
                     /gene="purM"
                     /locus_tag="J1Y83_002327"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HBA7630472.1"
                     /translation="MTDKTSLSYKDAGVDIDAGNALVGRIKGVVKKTRRPEVMGGLGG
                     FGALCALPQKYREPVLVSGTDGVGTKLRLAMDLKRHDTIGIDLVAMCVNDLVVQGAEP
                     LFFLDYYATGKLDVDTASAVISGIAEGCLQSGCSLVGGETAEMPGMYHGEDYDVAGFC
                     VGVVEKSEIIDGSKVSDGDVLIALGSSGPHSNGYSLVRKILEVSGCDPQTTELDGKPL
                     ADHLLAPTRIYVKSVLKLIEKVDVHAIAHLTGGGFWENIPRVLPDNTQAVIDEFSWQW
                     PEVFNWLQTAGNVERHEMYRTFNCGVGMIIALPAPEVDKALALLNANGENAWKIGIIK
                     ASDSEQRVVIE"
     gene            8242..8868
                     /gene="upp"
                     /locus_tag="J1Y83_002328"
     CDS             8242..8868
                     /gene="upp"
                     /locus_tag="J1Y83_002328"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HBA7630473.1"
                     /translation="MKIVEVKHPLVKHKLGLMREQDISTKRFRELASEVGSLLTYEAT
                     ADLETEKVTIEGWNGPVEIDQIKGKKITVVPILRAGLGMMDGVLENVPSARISVVGMY
                     RNEETLEPVPYFQKLVSNIDERMALIVDPMLATGGSVIATIDLLKKAGCSSIKVLVLV
                     AAPEGIAALEKAHPDVELYTASIDQGLNEHGYIIPGLGDAGDKIFGTK"
     gene            8954..10243
                     /gene="uraA"
                     /locus_tag="J1Y83_002329"
     CDS             8954..10243
                     /gene="uraA"
                     /locus_tag="J1Y83_002329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HBA7630474.1"
                     /translation="MTRRAIGVSERPPLLQTIPLSLQHLFAMFGATVLVPVLFHINPA
                     TVLLFNGIGTLLYLFICKGKIPAYLGSSFAFISPVLLLLPLGYEVALGGFIMCGVLFC
                     LVSFIVKKAGTGWLDVLFPPAAMGAIVAVIGLELAGVAAGMAGLLPAEGQTPDSKTII
                     ISITTLAVTVLGSVLFRGFLAIIPILIGVLVGYALSFAMGIVDTTPIINAHWFALPTL
                     YTPRFEWFAILTILPAALVVIAEHVGHLVVTANIVKKDLLRDPGLHRSMFANGLSTVI
                     SGFFGSTPNTTYGENIGVMAITRVYSTWVIGGAAIFAILLSCVGKLAAAIQMIPLPVM
                     GGVSLLLYGVIGASGIRVLIESKVDYNKAQNLILTSVILIIGVSGAKVNIGAAELKGM
                     ALATIVGIGLSLIFKLISMLRPEEVVLDAEDADITDK"
     gene            10293..11039
                     /gene="hda"
                     /locus_tag="J1Y83_002330"
     CDS             10293..11039
                     /gene="hda"
                     /locus_tag="J1Y83_002330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA inactivator Hda"
                     /protein_id="HBA7630475.1"
                     /translation="MVNFSRFCEILVEVSLNTPAQLSLPLYLPDDETFASFWPGDNSS
                     LLAALQNVLRQEHSGYIYLWAREGAGRSHLLHAACAELSQRGDAVGYVPLDKRTWFVP
                     EVLDGMEHLSLVCIDNIECIAGDELWEMAIFDLYNRILESGKTRLLITGDRPPRQLNL
                     GLPDLASRLDWGQIYKLQPLSDEDKLQALQLRARLRGFELPEDVGRFLLKRLDREMRT
                     LFMTLDQLDRASITAQRKLTIPFVKEILKL"
     gene            complement(11177..11536)
                     /gene="arsC"
                     /locus_tag="J1Y83_002331"
     CDS             complement(11177..11536)
                     /gene="arsC"
                     /locus_tag="J1Y83_002331"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311384.1"
                     /note="This arsenate reductase requires both glutathione
                     and glutaredoxin to convert arsenate to arsenite, after
                     which the efflux transporter formed by ArsA and ArsB can
                     extrude the arsenite from the cell, providing resistance;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase (glutaredoxin)"
                     /protein_id="HBA7630476.1"
                     /translation="MTKQVKIYHNPRCSKSRETLNLLKENGVEPEVVLYLETPADAAT
                     LRDLLKMLGMNSARELMRQKEDLYKELNLADSSLSEEALIQAMVDNPKLMERPIVVAN
                     GKARIGRPPEQVLEIVG"
     gene            complement(11557..13020)
                     /gene="bepA"
                     /locus_tag="J1Y83_002332"
     CDS             complement(11557..13020)
                     /gene="bepA"
                     /locus_tag="J1Y83_002332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-barrel assembly-enhancing protease"
                     /protein_id="HBA7630477.1"
                     /translation="MFRQLKKNLVATLIAAMTIGQVAPAFADSADTLPDMGTSAGSTL
                     SIGQEMQMGDYYVRQLRGSAPLINDPLLTQYINSLGMRLVSHANSVKTPFHFFLINND
                     EINAFAFFGGNVVLHSALFRYSDNESQLASVMAHEISHVTQRHLARAMEDQQRSAPLT
                     WVGALGSILLAMASPQAGMAALTGTLAGTRQGMISFTQQNEQEADRIGIQVLQRSGFD
                     PQAMPTFLEKLLDQARYSSRPPEILLTHPLPESRLADARNRANQMRPIVVQSSEDFYL
                     AKVRTLGMYNSGRNQLTSDLLDEWAKGNVRQQRAAQYGRALQAMEANKYDEARKTLQP
                     LLAAEPGNAWYLDLATDIDLGQNKANDAINRLKNARDLRTNPVLQLNLANAYLQGGQP
                     QEAANILNRYTFNNKDDSNGWDLLAQAEAALNNRDQELAARAEGYALAGRLDQAISLL
                     SSASSQVKLGSLQQARYDARIDQLRQLQERFKPYTKM"
     gene            13233..14294
                     /locus_tag="J1Y83_002333"
     CDS             13233..14294
                     /locus_tag="J1Y83_002333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HBA7630478.1"
                     /translation="MLEMLMQWYRRRFSDPEAIALLVILVAGFGIIFFFSGLLAPLLV
                     AIVLAYLLEWPTVRLQSIGCSRPWATSIVLILFVGILLLMAFVVLPIAWQQGIYLIRD
                     MPGMLNKLSDFAATLPRRYPALMDAGIIDAMAENMRSRMLTMGDSVVKISLASLVGLL
                     TIAVYLVLVPLMVFFLLKDKEQMLNAVRRVLPRNRGLAGQVWKEMNQQITNYIRGKVL
                     EMIVVGIATWLGFLLFGLNYSLLLAVLVGFSVLIPYIGAFVVTIPVVGVALFQFGAGT
                     EFWSCFAVYLIIQALDGNLLVPVLFSEAVNLHPLVIILSVVIFGGLWGFWGVFFAIPL
                     ATLIKAVIHAWPDGQIAQD"
     gene            complement(14393..14863)
                     /gene="bcp"
                     /locus_tag="J1Y83_002334"
     CDS             complement(14393..14863)
                     /gene="bcp"
                     /locus_tag="J1Y83_002334"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-dependent thiol peroxidase"
                     /protein_id="HBA7630479.1"
                     /translation="MNPLKAGDIAPKFSLPDQDGEQVNLTDFQGQRVLVYFYPKAMTP
                     GCTVQACGLRDNMDELKKAGVDVLGISTDKPEKLSRFAEKELLNFTLLSDEDHQVCEQ
                     FGVWGEKSFMGKTYDGIHRISFLIDADGKIEHVFDDFKTSNHHDVVLNWLKEHA"
     gene            complement(14863..15435)
                     /locus_tag="J1Y83_002335"
     CDS             complement(14863..15435)
                     /locus_tag="J1Y83_002335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system transcriptional
                     repressor"
                     /protein_id="HBA7630480.1"
                     /translation="MTLSSQHYLVITALGADRPGIVNTITRHVSSCGCNIEDSRLAML
                     GEEFTFIMLLSGSWNAITLIESTLPLKGAELDLLIVMKRTTARPRPPMPASVWVQVDV
                     ADSPHLIERFTALFDAHHMNIAELVSRTQPAENERAAQLHIQITAHSPASADAANIEQ
                     AFKALCTELNAQGSINVVNYSQHDEQDGVK"
     gene            15581..16459
                     /gene="dapA"
                     /locus_tag="J1Y83_002336"
     CDS             15581..16459
                     /gene="dapA"
                     /locus_tag="J1Y83_002336"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001311023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HBA7630481.1"
                     /translation="MFTGSIVAIVTPMDEKGNVCRASLKKLIDYHVASGTSAIVSVGT
                     TGESATLNHDEHADVVMMTLELADGRIPVIAGTGANATAEAISLTQRFNDSGIVGCLT
                     VTPYYNRPSQEGLYQHFKAIAEHTDLPQILYNVPSRTGCDLLPETVGRLAKVKNIIGI
                     KEATGNLTRVNQIKELVSDDFVLLSGDDASALDFMQLGGHGVISVTANVAARDMAQMC
                     KLAAEGHFAEARVINQRLMPLHNKLFVEPNPIPVKWACKELGLVATDTLRLPMTPITD
                     SGRETVRAALKHAGLL"
     gene            16476..17510
                     /gene="bamC"
                     /locus_tag="J1Y83_002337"
     CDS             16476..17510
                     /gene="bamC"
                     /locus_tag="J1Y83_002337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamC"
                     /protein_id="HBA7630482.1"
                     /translation="MAYSVQKSRLAKVAGVSLVLLLAACSSDSRYKRQVSGDEAYLEA
                     APLAELHAPAGMILPVTSGDYAIPVTNGSGAVGKALDIRPPAQPLALVSGARTQFTGD
                     TASLLVENGRGNTLWPQVVSVLQAKNYTITQRDDAGQTLTTDWVQWNRLDEDEQYRGR
                     YQISVKPQGYQQAVTVKLLNLEQAGKPVADAASMQRYSTEMMNVISAGLDKSATDAAN
                     AAQNRASTTMDVQSAADDTGLPMLVVRGPFNVVWQRLPAALEKVGMKVTDSTRSQGNM
                     AVTYKPLSDSEWHELGASDPGLASGDYKLQVGDLDNRSSLQFIDPKGHTLTQSQNDAL
                     VAVFQAAFSK"
     gene            17723..18436
                     /gene="purC"
                     /locus_tag="J1Y83_002338"
     CDS             17723..18436
                     /gene="purC"
                     /locus_tag="J1Y83_002338"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HBA7630483.1"
                     /translation="MQKQAELYRGKAKTVYSTENPDLLVLEFRNDTSAGDGARIEQFD
                     RKGMVNNKFNYFIMSKLAEAGIPTQMERLLSDTECLVKKLDMVPVECVVRNRAAGSLV
                     KRLGIEEGIELNPPLFDLFLKNDAMHDPMVNESYCETFGWVSKENLARMKELTYKAND
                     VLKKLFDDAGLILVDFKLEFGLYKGEVVLGDEFSPDGSRLWDKETLEKMDKDRFRQSL
                     GGLIEAYEAVARRLGVQLD"
     gene            18604..19467
                     /locus_tag="J1Y83_002339"
     CDS             18604..19467
                     /locus_tag="J1Y83_002339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="neutral zinc metallopeptidase"
                     /protein_id="HBA7630484.1"
                     /translation="MRWQGRRESDNVEDRRNSSGGPSMGGPGFRLPSGKGGLILLIVV
                     LVAGYYGVDLTGLMTGQPVSQQQSTRSISPNEDEAAKFTSVILATTEDTWGQQFEKMG
                     KTYQQPKLVMYRGMTRTGCGAGQSIMGPFYCPADGTVYIDLSFYDDMKDKLGADGDFA
                     QGYVIAHEVGHHVQKLLGIEPKVRQLQQNATQAEVNRLSVRMELQADCFAGVWGHSMQ
                     QQGVLETGDLEEALNAAQAIGDDRLQQQSQGRVVPDSFTHGTSQQRYSWFKRGFDSGD
                     PAQCNTFGKSI"
     gene            19482..21497
                     /locus_tag="J1Y83_002340"
     CDS             19482..21497
                     /locus_tag="J1Y83_002340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA(Met) cytidine acetyltransferase"
                     /protein_id="HBA7630485.1"
                     /translation="MAELTALHTLTAQMKREGIRRLLVLSGEEGWCFDHALKLRDALP
                     GDWLWISPQPDAENHCSPSALQTLLGREFRHAVFDARHGFDAAAFAALSGTLKAGSWL
                     VLLLPVWEEWENQPDADSLRWSDCPDPIATPHFVQHLKRVLTADNDAILWRQNQPFSL
                     AHFTPRTDWHPATGTPQPEQQQLLQQLLTMPLGVAVVTAARGRGKSALAGQLISRIAG
                     SAIVTAPAKAATDVLAQFAGEKFRFIAPDALLASDEQADWLVVDEAAAIPAPLLHQLV
                     SRFPRTLLTTTVQGYEGTGRGFLLKFCARFPHLHRFELQQPIRWAQRCPLEKMVSEAL
                     VFDDENFTHTPQGNIVISAFEQTLWRSEPETPLKVYQLLSGAHYRTSPLDLRRMMDAP
                     GQHFLQAAGENEIAGALWLVDEGGLSQELSQAVWAGYRRPRGNLVAQSLAAHGSNPLA
                     ATLRGRRVSRIAVHPARQREGTGRQLIAGALQYIHDLDYLSVSFGYTEELWRFWQRCG
                     FVLVRMGNHREASSGCYTAMALLPMSDAGKQLAEREHYRLRRDAQALAQWNGEMLPVD
                     PLNDAVLSDDDWLELAGFAFTHRPLLTSLGCLLRLLQTSELALPALRGRLQKNASDAQ
                     LCTTLKLSGRKLLLVRQREEAAQALFALDDVRTERLRDRITQWQFFH"
     gene            21571..22269
                     /gene="ypfH"
                     /locus_tag="J1Y83_002341"
     CDS             21571..22269
                     /gene="ypfH"
                     /locus_tag="J1Y83_002341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311362.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HBA7630486.1"
                     /translation="MKHDHFVVQSPDKPAQQLLLLFHGVGDNPVAMGEIGSWFAPLFP
                     DALVVSVGGAEPSGNPAGRQWFSVQGITEDNRQARVDAIMPTFIETVRYWQKQSGVGA
                     NATALIGFSQGAIMALESIKAEPGLASRVIAFNGRYASLPETASTATTIHLIHGGEDP
                     VIDLAHAVAAQEALISAGGDVTLDIVEDLGHAIDNRSMQFALDHLRYTIPKHYFDEAL
                     SGGKPGDDDVIEMM"
     gene            complement(22350..22550)
                     /locus_tag="J1Y83_002342"
     CDS             complement(22350..22550)
                     /locus_tag="J1Y83_002342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpfN family protein"
                     /protein_id="HBA7630487.1"
                     /translation="MDWLAKYWWILVIVFLVGVLLNVIKDLKRVDHKKFLANKPELPP
                     HRDFNDKWDDDDDWPKKDQPKK"
     gene            complement(22578..23705)
                     /gene="dapE"
                     /locus_tag="J1Y83_002343"
     CDS             complement(22578..23705)
                     /gene="dapE"
                     /locus_tag="J1Y83_002343"
                     /EC_number="3.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020078199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinyl-diaminopimelate desuccinylase"
                     /protein_id="HBA7630488.1"
                     /translation="MSCPVIELTQQLIRRPSLSPDDAGCQALLIERLQAIGFTVERMD
                     FADTQNFWAWRGQGETLAFAGHTDVVPPGDADRWINPPFEPTIRDGMLFGRGAADMKG
                     SLAAMVVAAERFVAQHPNHTGRLAFLITSDEEASAHNGTVKVVEALMARNERLDYCLV
                     GEPSSIEVVGDVVKNGRRGSLTCNLTIHGVQGHVAYPHLADNPVHRAAPFINELVAIE
                     WDQGNEFFPATSMQIANIQAGTGSNNVIPGELFVQFNFRFSTELTDEMIKAQVLALLE
                     KHQLRYTVDWWLSGQPFLTARGKLVDAVVNAVEHYNEIKPQLLTTGGTSDGRFIARMG
                     AQVVELGPVNATIHKINECVNAADLQLLARMYQRIMEQLVA"
     gene            complement(23709..24065)
                     /locus_tag="J1Y83_002344"
     CDS             complement(23709..24065)
                     /locus_tag="J1Y83_002344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsC family reductase"
                     /protein_id="HBA7630489.1"
                     /translation="MVTLYGIKNCDTIKKARRWLEANNIDYRFHDYRVDGLDSELLNG
                     FINELGWEALLNTRGTTWRKLDETTRNKITDAASAATLMTEMPAIIKRPLLCAPGKPM
                     LLGFSESSYQQFFHEV"
BASE COUNT         5644 a         6010 c         6351 g         6157 t
ORIGIN      
        1 aaacgtaata attcattaat cagggttatt ttgtttgata tatatcaatt ggaactttca
       61 tatgacactt agaatcattg ctctctatag tgatgaataa tcatcattcg aagtcaggtg
      121 ggatgcctgt ctgaatacac ctccttcagg atgtggggga ttctgctgag catctatgaa
      181 actgaatgca acttatataa aaatacgtga taaatggtgg gggcttccgc tgttcctgcc
      241 ttctttaatc ttgcccattt tcgcccacat taatactttc gcgcatattt cttccggtga
      301 agtttttctc ttttatctgc cactggcact gatgatcagc atgatgatgt ttttcagctg
      361 ggcggcattg ccagggatcg ccttagggat ttttgtccgc aaatatgcag agctgggttt
      421 ttacgaaacg ctctcattaa cggctaattt tattatcatt atcattctct gttggggcgg
      481 ttacagggtt tttacccccc ggcgtaacaa cgtttcacat ggtgatagcc gtttaatttc
      541 ccagcgacta ttctggcaga ttgtgtttcc tgcaacgctg tttctgatac ttttccagtt
      601 tgctgcgttt gtagggttac tggcgagcag agaaaatctg gtcggcgtca tgccctttaa
      661 cctcgggacc ttaatcaatt atcaggcctt gctggtgggt aatctgattg gtgtcccgct
      721 gtgctacttc atcattcggg tggtgcgaaa tccgttttat ttacgtagct attattcgca
      781 attaaaacag caggttgatg ccaaagttac caaaaaagag ttcgcaatct ggctactggc
      841 attaggtgcc ttattattgc tgttatgcat gccgttaaat gaaaaaagca cgatttttag
      901 caccaattac accttgtcat tattgctgcc cctgatgatg tggggagcga tgcgttatgg
      961 ttataagctg atttcattgc tctgggcggt cgtgttgatg atcagcatcc acagctatca
     1021 aaattacatt cccatttatc ctggctatac cacgcagctg accataacct cctccagtta
     1081 tctggtattc tcttttattg tcaattatat ggctgtactg gcaacccgtc agcgagcggt
     1141 agtcagacgc attcagcggc ttgcgtatgt ggacccggtg gttcatctgc caaatgttcg
     1201 cgccctgaat cgcgcgttac gtgatgcccc ctggtctgcg ctttgttatt tacgcatccc
     1261 tggcatggaa atgctggtta agaattatgg catcatgctg cggattcaat acaagcaaaa
     1321 actttctcac tggctgtcac ccttgctgga accgggtgaa gatgtttatc agctttcggg
     1381 taacgatctc gcgctgcggc tgaatacaga atcgcaccag gagcgcatta ccgcactgga
     1441 tagccatctc aagcaatttc gtttcttttg ggatggaatg ccgatgcaac cgcagattgg
     1501 cgtcagttac tgctatgtgc gctcgccagt gaatcatatc tacctgctgc tgggagagct
     1561 aaatacggtg gccgaacttt ccatcgtgac caacgccccg gaaaatatgc agcgtcgcgg
     1621 agcaatgtat ttgcaacgcg aattgaaaga taaagtcgcg atgatgaatc ggctacagcg
     1681 ggcgctggaa cacaaccatt ttttcctgat ggctcagccg attaccggta tgcgtggtga
     1741 tgtctaccat gaaattcttc tgcgcatgaa aggtgagaat gatgaactga tcagccccga
     1801 tagcttctta ccggtcgcgc acgaatttgg tttatcgtcg agtatcgaca tgtgggtcat
     1861 tgagcatacg ctgcaattta tggctgaaaa cagagcgaag atgcccgctc accgttttgc
     1921 tattaatctg tctccaacct cggtatgtca ggctcgtttt cctgttgaag tcagtcagct
     1981 gctggctaaa tatcagattg aagcgtggca acttattttt gaagtcaccg aaagtaatgc
     2041 tctgaccaat gttaagcagg cgcaaatcac cttgcagcat cttcaggaat taggctgcca
     2101 gattgcgatt gatgatttcg gcaccggcta cgccagctat gcgcggctta aaaatgtgaa
     2161 tgccgatctg cttaaaattg acggcagttt tatccgcaat attgtgtcaa atagtctgga
     2221 ttatcagata gtggcgtcga tttgccactt ggcgcgaatg aagaaaatgc gggtagtggc
     2281 agagtacgtt gaaaacgaag agatccgcga ggcggtgctc tctttgggga tcgattatat
     2341 gcagggttat cttattggta agccgcaacc gttaattgat acgctgaatg aaatcgaacc
     2401 cattcgcgaa agtgcctgaa taatgcgggc cgacatttct cgtcggcccg caaagcatta
     2461 agcggcgatt tctggtgtac tttcttcttc aattttcaac cgccagccag ccacgccttc
     2521 ccagtattct tgctcctttt ccagatcaag cagtaccagc gcattctgac taaaccagtc
     2581 atgcgggaaa cgcagtgtcc agtgactgtc atcagtaatc agcgtcaatg ttggcggtgt
     2641 ggtggttgcc tgacgttgat tattgagtaa tacgccaagg cgcaatagct gaatcagtgg
     2701 caggaactgt ttcttcttaa acaaggtaaa gcgcggcaga tcgtcgagtt taatcgcttt
     2761 acggtgatag cgcaccagtg tcgccatcat cagctgctgt tcctgattaa aacccggcaa
     2821 gtcgctgttt tgcagaatat aagcggagtg gcgatgcaaa ccgctgtggt tgatattcaa
     2881 cccgacctca tgcagcatgg cggcccatcg cagtagcgcc tccagttgtg gatgcgccag
     2941 cttcggttgc tgttcccgcc actgttcgta catttgcata gtggtatcca gcacccgtcg
     3001 ggcctgttcg ctgtcgatgt gatactggtt ggcgaggctg ctggcagtgc gactacgcac
     3061 atcctgatga cggaaacgtc cttccatttc atacagtacg ccttcgcgta acgccccgtc
     3121 agaaaggcgc agttcacgga tggctaaagc atcaaacaca ccgcataaaa tcgccagtcc
     3181 aggaacgaag actgttttcc gctcttcgga aagacctggt aaactcagcg atgcgaaatt
     3241 acggtgccgt aaaacttctt ttaccagttt ttccagacgt tccggggtaa ttatcccgtc
     3301 tttctcgccc atttccatta acacttcatg ggcggctttt atggtaccgg aagcgcccat
     3361 tgccacgttc cagccctgaa ttcgaaattg ccaggttaac gtttccagtt tttgtgctgc
     3421 cgccatgcga gcgcgctgaa aattctcttt attgatgacc ccgccaggga aataaagctg
     3481 ggcaaagctg acacaaccca tccggcggct ttcaacgaga atcggttcga aattttcacc
     3541 aatcaccagt tctgtagaac cgccgccaat atcaataacc agtttgcgac ctttttccgg
     3601 ttgggtatgt tccacgccca taaaaatcag acgggcttct tcattaccgg aaataatttc
     3661 gatcgggtag ggaatgactt tttccgcgcg tttcagaaag tcagtggcgt tcagcgcctg
     3721 acgcagcgta tgggtaccaa ctatacagac gctggcagga gaaaaccctt gtagcctttc
     3781 ggcaaatagc gacagacagt tcaaaccgcg cgtcattgcc tcttcactca acatattatc
     3841 tggccccagg ccgtccgcca gatgcacccg ctgtttcagg cggccaataa tctgcatggc
     3901 accatctacc acacgggcta tgaccatgtg aaaactgttt gaaccaagat cgaccgcagc
     3961 aaactcctgc ggacgagggg atttatcgtg tattggcata gggttattca gattgttcga
     4021 gtgatttgat gtagtcgtaa atcgccaact gcgcccgtac tttgcggcga ttgccgcggg
     4081 gaacgtagcg attactgagt tctttatcga tataacgtgc tttgaccgta tcgctgaaca
     4141 atatgtcgat gatgtccagc acccgctgct tcaggcgcgg atcgagcagc ggcgttgcca
     4201 cttcaatacg ataatcaata ttgcgcgtca tccagtcggc ggaagaaagg tagacctttt
     4261 tatcgccgcc attttcaaaa atataaaccc ggtcatgttc aaggtaacgg tcaacaatac
     4321 tgatggcacg aatgttgtcg ctaatgcctt ccagattggg gatcagagaa cacattccgc
     4381 gcaccagcag attaaccggt acgccggagc tggaggccgc atacagacga tcaaccaggc
     4441 ctttatcgac aaggttattt agcttcaggg tgataccact gggcagccct tgctgcgcgt
     4501 tggcgatctc gcggtccacc atttcataca acagacggcg agagttttgc ggcgacacca
     4561 ttaaataatc aaatgtcacc ggacggtatg ggttttcaat aaagttgaac acccgccgta
     4621 cttcgttagt gatgcgcgca tcggcggtca gcaacgaata gtcagtataa agacgcgcgg
     4681 ttttttcgtt aaagttcccg gtcccgatgt gtgcgtaacg caccacttca ccgttttctt
     4741 tacgtgaaat caggaacagt ttggcgtgaa ttttcagccc cggcgcagag aagataacgt
     4801 gcacgcctgc ttcggtcagg cgcttcgccc agtgaatgtt ggcttcttcg tcgaaacgcg
     4861 cctgtaactc aaccaccacg gtgactttct taccgttgtg tgcggcgtgg atcatcgagt
     4921 cgatgatgcg tgaatctttc gctacgcggt agatattgat ttttatcgcc agcacgctcg
     4981 ggtcgaacga cgcctgacgt agcagttcca gcacgtgctc aaaggtgtga taaggataat
     5041 agagcaacac atcgcgttcg cgaatggcat caaaaccatt gcggaactgg gctttatcaa
     5101 accaaatatg gcgtaaacgc ggcagtggtt tgttcaccag attggctttg ccgacattgg
     5161 ggaaattaat aaagtcttta aaattatgat aacgaccgcc ggggacgatg gagtcgtagc
     5221 gggaaatagt cagtttttcg cgtaacactt caaccagagc attgggcata tcgcgctgat
     5281 aaacaaaacg caccggctca gcagttaaac gctgcttgag actggaagac atcaactcca
     5341 tcagactggc ttccatctca tgcactaaat cgtattcggc atcgcgggtc atcttcattg
     5401 aataggcatt cagtgcgtca taatcaaaga agcctttgaa aatatcatca aggcagtaac
     5461 gcagaatgtt atccagaaga atcatcggct tgcgtcgacg cggcgcttct ggcggtaaat
     5521 tcacaaagcg cggcacttta tctgatggga tctccagcag cgcgtaacgg atggtatcgc
     5581 cacggataat ttccaccgcc agataggtgt aatcatcttt caggaactgc actaagtcag
     5641 tgtcaggatt gattaaaatc ggcgtaatgt gctgacgcag atactgctta aaataatgac
     5701 gcagccagat ttgttgattg acggagagct ggcgttcatt aatcaggaag atctggttgc
     5761 gcgccatttc cagcagtagc tcgttgtaga ggccgtcgaa ttcctgatcg gctttcagca
     5821 cacgggactg aattttgccc agtaaatggc gggaatgaga gttagagcct tgttcttcgc
     5881 taataatgat gcgtcgcttc agttcagcga agcggacttt atagaactca tcaaggttat
     5941 tggaatagat ccccaggaaa cgcatccttt caatcagcgg gttagatttg tccgccgctt
     6001 cctgaagcac gcgttcattg aacgataacc aactgagctc tttttcgatg tatagctttt
     6061 cctgacccat taccactttt actccgtttt attcacggga cactgcctgg atattatggc
     6121 gagcaatatt ggcagatgtc caactatgcc agaaaagggg gaaaaaaact gctggagctt
     6181 gcgatttaca ggtgaattga aaaacgccag cggcagagct ggcgctttaa ctacgggggc
     6241 attactcgtc ggcagcgtag ccctgcggcg gcagacgttg accatccagc cacgcggcgt
     6301 tttcgtgcat tttcagacga ccatcggcaa accagctaat caccagtgga taaatagcgt
     6361 gttcctgggt ttgcacgcgg gcggtgatgt catcttccgt atcaccagca aataccggga
     6421 ctttcgcctg taaaataacc gggccaccgt ccagttcatc ggtgacgaaa tgcaccgatg
     6481 taccgtgctc ctcatcgcca ttttccagcg cctgacgatg ggtgtgtaat ccgggatatt
     6541 tcggcagcag agaagggtga atgttcagca aacgcccggc atagtgggag acaaacgccg
     6601 ggctgagaat gcgcataaaa ccagccagca cgaccacatc gggtgcgtac atgtcgattt
     6661 catgaatcaa ctcccggtca taggcttcac gactgtcaaa cgcgctggtg atgagcgtat
     6721 gcgttgcaat acccgcctgg cgggcgcgtt caaggccgaa cgcgtcggcc ttattgctga
     6781 aaactgcccg tacggtgcct ttaattttgt tggttttgca ggcgtcaata attgcctgca
     6841 aattacttcc gttgccggaa ataagcacca caatattcat tattcgataa ccacgcgttg
     6901 ttcggaatca gaggctttga tgataccgat tttccacgcg ttttcaccgt tggcattgag
     6961 cagggcgagg gctttgtcca cttccggagc aggcagggca ataatcatac cgacgccgca
     7021 gttgaaggtg cgatacattt catggcgctc aacgttacct gccgtttgca gccagttaaa
     7081 cacttccggc cactgccagg aaaattcatc aatcactgcc tgggtattat ctggcaatac
     7141 gcgcggaatg ttttcccaga aaccgccgcc ggtcaggtgc gcaatggcat gcacatcgac
     7201 cttttcaatc aacttcagca ctgacttcac gtaaatgcgg gtcggtgcca gcagatgatc
     7261 ggctaatggc ttaccatcaa gttcggtggt ttgcggatca caaccgctga cttcaagaat
     7321 tttgcgcacc agcgaatagc cattcgagtg cgggccgctg gagccgagtg caatcagcac
     7381 atcgccgtcg ctgactttag agccgtcaat gatttctgat ttttctacca cgccaacgca
     7441 gaaaccagcg acatcgtaat cttcaccgtg atacatcccc ggcatttccg ccgtttcgcc
     7501 acccaccagt gaacagcccg attgcagaca accttccgca atgccgctga tcaccgctga
     7561 agcggtatca acatccagtt ttccggttgc gtaatagtcg aggaaaaaca gcggttccgc
     7621 accttgcacc accaggtcat taacgcacat ggcgaccaga tcaataccaa tggtgtcgtg
     7681 acgttttaag tccatcgcca gacgcagctt ggtacctacg ccgtcagtgc cggaaaccag
     7741 cacaggttca cgatattttt gcggcaatgc acacagcgca ccgaagccgc ccagaccgcc
     7801 catcacttcc ggacgacgcg ttttcttcac tacgcctttg attcttccaa ccagagcatt
     7861 acccgcgtca atatcaacac cggcatcttt gtagctaaga gaggttttat cggtcactgc
     7921 ttgggtcccc acgcgttact tgcggtagaa aaataaaatt cggcgcaatt ctaacaggga
     7981 aagcaaacgt ttgcgagact gctttacaca acctttttgc acgtcttttc cccaggcgcg
     8041 cggcgaaaga atacttgtac cagggcaaag gatcgttttc ggatggaata atcttcttcc
     8101 ataaccattt gaatataaaa taactttatc tcaaaccgtt atcattttga ctaaagtcaa
     8161 cgaaaataat attgccgcct tgaagaaagg aggtataatc cgtcgatttt ttttgtggct
     8221 gcccctcaaa ggagaaagag tatgaagatc gtggaagtca aacacccact cgtcaaacac
     8281 aagctgggac tgatgcgtga gcaagatatc agcaccaagc gctttcgcga actcgcttcc
     8341 gaagtgggta gcctgctgac ttacgaagcg accgccgacc tcgaaacgga aaaagtcact
     8401 atcgaaggct ggaacggccc ggtagaaatc gaccagatca aaggtaagaa aattaccgtt
     8461 gtgccaattc tgcgtgcggg tcttgggatg atggacggtg tgctggaaaa cgttccgagt
     8521 gcgcgcatca gtgtcgtcgg tatgtaccgt aatgaagaaa cgctggagcc ggtaccgtac
     8581 ttccagaaac tggtttctaa catcgatgag cgtatggcgc tgatcgttga cccaatgctg
     8641 gcaaccggtg gttccgttat cgcgaccatc gacctgctga aaaaagcggg ctgcagcagc
     8701 atcaaagttc tggtgctggt agctgcacca gaaggtatcg ctgcgctgga aaaagcgcac
     8761 ccggacgtcg aactgtatac tgcatcgatt gaccagggac tgaacgagca cggatacatt
     8821 attccgggcc tcggcgatgc cggtgacaaa atctttggta cgaaataaag aataaaaata
     8881 attaaagccg actttaagag tcggcttttt tttgagtaaa gcgcctataa cacataatac
     8941 agaggataat actatgacgc gccgtgctat cggggtgagt gaaagaccgc cacttttaca
     9001 gacaatcccg cttagtttgc aacatttgtt cgccatgttt ggtgcaaccg tcctggtgcc
     9061 cgtcttattt catattaacc cggcgactgt actgttattt aacggtatag gaacactgct
     9121 gtatctcttc atctgtaaag ggaaaattcc ggcttatctt ggttccagct ttgcctttat
     9181 ttcaccggta ttgttactgt tgccgttggg gtatgaagtt gcgctgggcg gctttattat
     9241 gtgcggcgtg ctgttctgcc tggtttcttt tatcgtgaag aaagcgggga ccggctggct
     9301 ggacgtgctg tttccacctg cggcaatggg cgcaatcgtt gccgtcatcg gtctggagct
     9361 ggcgggcgta gctgccggta tggcgggttt actcccggct gaagggcaaa cgccagactc
     9421 caaaaccatc atcatctcta ttaccaccct ggcggtcacg gttttaggtt ccgtgctgtt
     9481 tcgtggtttc ctggcaatta tcccgatttt aattggcgtg ctggtggggt acgcgctctc
     9541 tttcgcaatg ggaattgtcg ataccacgcc gattattaat gctcactggt ttgcgctgcc
     9601 gaccctctat acgccgcgct tcgagtggtt tgccattctg actattctgc cagcggcgtt
     9661 agtggttatt gccgaacacg tagggcacct ggtcgtaacg gctaatatcg tcaaaaaaga
     9721 tctgctgcgc gatccaggtc tgcaccgttc gatgtttgct aacggcttgt cgaccgtgat
     9781 ctccggtttc tttggttcca caccaaacac cacctatggg gaaaacatcg gtgtgatggc
     9841 gatcacccgt gtttacagta cctgggttat cggcggtgcg gcgattttcg ctatcctgct
     9901 ttcctgcgtc ggtaaactgg ctgctgctat ccagatgatt ccattgccgg tgatgggcgg
     9961 cgtttcgctg ctgctttacg gggtcatcgg tgcttccggt attcgtgtgt tgatcgaatc
    10021 gaaagtggat tacaacaaag cacagaacct gatcctgact tccgttattt tgatcatcgg
    10081 cgtcagtggt gcgaaggtaa acattggcgc ggcggagctg aaaggtatgg cgctggcgac
    10141 catcgtcggt atcggcttaa gtttgatctt caaactgatt agtatgctgc gccctgaaga
    10201 agtggtgctg gacgcagaag acgcggatat aacagacaaa taatgtaccg accgggcagt
    10261 gttgctgccc ggttcaaaca tcatgggatt ctgtggtaaa cttctcgcga ttttgtgaaa
    10321 tcctggttga ggtatctctg aacacaccgg cacagctctc tttgccactt tatcttcctg
    10381 acgacgaaac ctttgcaagt ttctggccgg gggataactc ctctttactg gccgcgctgc
    10441 aaaacgtgct gcgtcaggaa catagcggtt acatctatct ctgggcacgc gaaggcgcgg
    10501 ggcgcagcca tctgctgcac gcggcttgcg cggaattgtc acagcgtggc gatgcggtgg
    10561 gctatgtccc gctggataaa cgcacctggt ttgttccgga agtgctcgac ggtatggagc
    10621 atttgtcgct ggtctgtatt gacaacattg agtgtattgc cggcgatgag ttgtgggaga
    10681 tggcgatttt cgatctctac aatcgaattc tggaatcggg caaaacacgg ttgttgatca
    10741 ccggcgatcg tccaccgcgg cagttgaatc tgggattacc ggatctcgcg tcgcgtctcg
    10801 actgggggca gatctacaaa ttgcagccac tttctgatga agataagttg caggcgctac
    10861 agttacgcgc gcgtttgcgt ggttttgaac tgccggaaga tgtggggcgt ttcttgctga
    10921 agcggctcga cagagaaatg cgcacgttgt ttatgacgct ggatcagctg gatcgtgcgt
    10981 cgattaccgc gcaacgcaag ctgaccattc cttttgtgaa agaaattctg aagttgtaga
    11041 taaggtgttt attgtcggat gcggcgcgaa cgccttatcc gacctacgcg aagttaatcc
    11101 atagtattcg taggccggat aagacgcggc aagcgtcgca tccggccatg tgcttcaact
    11161 agcgatggag cacatcttaa cccacgattt ccagtacctg ctccggtgga cgaccaatcc
    11221 gtgctttgcc attcgcgacc acaatcgggc gttccattag cttcggatta tccaccatcg
    11281 cctgaatcag cgcctcttcg ctaagtgaac tgtcagccag gttcagttct ttataaagat
    11341 cctctttttg gcgcatcaat tcacgagcgc tattcatccc cagcattttc agcaaatcac
    11401 gcagcgttgc cgcatcggcg ggtgtctcaa ggtagagcac cacttctggc tccacgccgt
    11461 tttctttcag caggttcagc gtttcccggc tctttgaaca gcgtgggtta tggtagattt
    11521 ttacctgttt ggtcattgtt cttcctttaa tgcgaattac atcttggtat aaggcttaaa
    11581 gcgttcctgc agctggcgca actggtcgat gcgcgcatcg taacgcgctt gttgcaggct
    11641 gcctaatttc acctgagaac tggcgctact caacaaggaa atagcctgat cgagtcgccc
    11701 ggcgagcgca taaccttctg ctcgcgcagc cagttcctga tcgcggttat ttagtgcggc
    11761 ttccgcctgt gccagtaaat cccagccgtt gctgtcatct ttattgttaa aagtgtagcg
    11821 attcagaata ttggccgctt cctgtggttg ccccccttgc agataagcgt tcgccaggtt
    11881 gagctgcaac accggattgg tgcgcaaatc gcgggcattt ttcaggcgat tgatagcgtc
    11941 attggctttg ttttgcccaa gatcgatatc ggtagccaga tcgagatacc atgcgttgcc
    12001 aggttctgcc gccagtaacg gttgcagcgt tttacgcgct tcgtcgtatt tattggcttc
    12061 catcgcctgt aaagcacgac catattgcgc cgctcgttgc tgacgaacgt ttcctttcgc
    12121 ccattcatcc agcaaatcac tggtgagttg gttacgccct gaattgtaca tccccagtgt
    12181 gcgcactttt gccagataga aatcttctga cgattgcacc actatcgggc gcatctgatt
    12241 ggcacggtta cgggcatcag ccagacggct ttccggtagt gggtgagtga gcagaatttc
    12301 tggcgggcgc gaagagtaac gcgcctgatc gagtaatttt tcgaggaagg tcggcatcgc
    12361 ctgcggatcg aatcccgagc gttgcagcac ctgaatacca atgcggtccg cttcctgttc
    12421 attttgctgg gtgaaactga tcatcccctg gcgcgttccc gccagtgtac cggtcagcgc
    12481 cgccatcccc gcctgcggac tggccatcgc cagtaaaata gaacctaacg cgccgaccca
    12541 ggtcagcggc gcgctgcgct gctgatcttc catcgctcgc gccaggtgac gttgggtgac
    12601 gtgggagatt tcgtgcgcca taactgaagc cagttgactt tcgttatcgg aataacggaa
    12661 cagggcagag tgcagcacca cgttgccgcc aaagaaagca aaggcgttaa tttcatcgtt
    12721 gttgatcaga aagaaatgaa acggtgtctt aaccgaattg gcatgcgaaa ccagacgcat
    12781 tcctagcgaa ttaatgtatt gcgttaacag cggatcatta attaacggcg cgctgccgcg
    12841 taactggcgg acataatagt cgcccatctg catttcctga ccaatggaaa gtgtgcttcc
    12901 tgcggaggtt cccatatccg gcaaggtgtc tgcgctgtcg gcaaacgccg gggctacctg
    12961 accaatggtc atagcagcaa tgagggttgc aaccaggttt tttttcaact gcctgaacat
    13021 aacctctatc ctgtatttct ggagatagtc atttgaccga tactgtgaaa tatcgttccc
    13081 accgcgccgc ctttgagtgt aacggcgaag attagcccac tcaaatgact tttcagtttc
    13141 ctgaatccgt cataaaaagc gaagtctttt ttgtgacatt tcgatacaat tgcggatcgc
    13201 aatcccgctc ttgaggttca gggaaggtct gtatgctcga aatgttgatg caatggtatc
    13261 gccgccgttt tagcgacccg gaagcgattg ccttgctggt tattttggtt gctggttttg
    13321 gcattatctt tttctttagc ggcctactcg cgccgttgct ggtggctatt gtgctggcct
    13381 atttactgga atggccgact gtccgcctgc agtctattgg ctgttcccgc ccttgggcga
    13441 catctatcgt gctgatcctg tttgtgggca tcctgctact gatggcgttc gtggtactgc
    13501 ctatcgcctg gcagcagggc atctacttaa tccgcgatat gccggggatg ctcaataagc
    13561 tttctgactt tgccgccacg ttgccgcgcc gctatccggc gttaatggat gcgggcatta
    13621 ttgatgcaat ggctgaaaat atgcgcagta ggatgctgac catgggcgat tcggtggtga
    13681 aaatttccct cgcctcgttg gttggtttgc tgaccattgc cgtctatctg gtgctggtgc
    13741 cattgatggt cttcttcctg ctgaaagaca aagagcagat gctgaacgcc gttcgccggg
    13801 tgctgccgcg caaccgtgga ctggcaggac aggtgtggaa ggagatgaat caacaaatca
    13861 ccaactatat ccgcggcaaa gtgctggaga tgatcgtggt agggatcgcc acctggctgg
    13921 ggttcttgct ctttgggctg aactattcgc tgctgctggc ggtgctggtc ggcttctcgg
    13981 tacttattcc gtacattggc gcatttgtgg tgaccattcc ggtggttggc gtggcgctat
    14041 tccagtttgg tgctggcacg gaattctgga gctgcttcgc ggtgtatctg attattcagg
    14101 cgctggacgg caacctgtta gtaccggtgt tgttctccga agcggttaac ctgcatcctc
    14161 tggtgattat tttatcggtg gtgatcttcg gtggtttgtg gggattctgg ggcgtattct
    14221 tcgctattcc attggcgacg ctgatcaaag ccgtgattca cgcctggcct gatgggcaaa
    14281 tcgcgcaaga ctgaccttca gcgaaaaaat tttaaacgag tgttgcaggg cagtgagaag
    14341 atttgagatg tgctggccgc aagcgcagcc agcacggaat ggagcaaagt aatcaggcgt
    14401 gttctttcag ccagttcagc acaacgtcgt ggtgattgct ggttttgaaa tcgtcaaaga
    14461 catgttcgat tttgccatca gcgtcaatca ggaagctgat gcgatgaata ccatcgtagg
    14521 ttttgcccat gaaggacttt tcaccccaga cgccgaattg ttcgcacacc tggtggtcct
    14581 catcagacag gagcgtaaag ttaagcagct ctttttccgc aaaacgggag agtttttcgg
    14641 gtttatcggt gctgataccc agcacatcaa cgcccgcttt tttcaactca tccatgttat
    14701 cgcgtaagcc gcaggcctgt acggtacagc cgggggtcat ggctttcggg tagaaataaa
    14761 ccagaacacg ctgtccctgg aagtcggtca aattaacttg ttctccgtct tgatccggca
    14821 agctaaattt cggtgcgata tcaccggctt tcagtggatt cattacttaa ctccatcctg
    14881 ttcatcatgt tgggaataat tgacgacgtt aatactgcct tgtgcattga gttctgtaca
    14941 tagggctttg aacgcttgct caatatttgc tgcgtccgca gatgcggggc tgtgggcagt
    15001 tatctgaata tgcaactgcg cagcccgttc attttcagca ggttgcgtgc gcgacaccag
    15061 ctccgcaatg ttcatatgat gcgcgtcgaa aagtgctgtg aagcgttcaa ttaaatgcgg
    15121 ggagtctgcc acatcgacct gaacccagac agatgctggc attggcggac gcggacgcgc
    15181 cgtcgtgcgc ttcatcacga ttaaaagatc cagctcggca cctttcaacg gtaacgttga
    15241 ttcaatcaga gtaatggcat tccatgaacc ggaaagcagc atgataaacg tgaactcttc
    15301 tcccagcatc gccaggcgac tgtcttcaat attacagccg caactactga catgacgggt
    15361 gatggtgttc actattccag ggcgatcggc acccaacgca gtgatcacca gataatgttg
    15421 cgatgacagt gtcaaactgg ttattccttc aaggggtgag ttgttcttaa ggaaagcata
    15481 aaaaaaacat gcatacaaca atcagaacgg ttctgtctgc ttgcttttaa tgccatacca
    15541 aacgtaccat tgagacactt gtttgcacag aggatggccc atgttcacgg gaagtattgt
    15601 cgcgattgtt actccgatgg atgaaaaagg taatgtctgt cgggctagct tgaaaaaact
    15661 gattgattat catgtcgcca gcggtacttc ggcgatcgtt tctgttggca ccactggcga
    15721 gtccgctacc ttaaatcatg acgaacatgc tgatgtggtg atgatgacgc tggagctggc
    15781 tgacgggcgc attccggtga ttgccgggac cggtgctaac gctactgcgg aagccattag
    15841 cctgacgcag cgcttcaatg acagcggtat cgtcggctgc ctgacggtaa ccccttacta
    15901 caatcgtccg tcgcaagaag gtttgtatca gcatttcaaa gccatcgctg aacatactga
    15961 cctgccgcaa attctgtata atgtgccgtc ccgtactggc tgcgatctgc tcccggaaac
    16021 ggtgggccgt ctggcgaaag taaaaaatat tatcggaatc aaagaggcaa cagggaactt
    16081 aacgcgtgta aaccagatca aagagctggt ttcagatgat tttgttctgc tgagcggcga
    16141 tgatgcgagc gcgctggact tcatgcaatt aggcggtcat ggggttattt ccgttacggc
    16201 taacgtcgca gcgcgtgata tggcccagat gtgcaaactg gcagcagaag ggcattttgc
    16261 cgaggcacgc gttattaatc agcgtctgat gccattacac aacaaactat ttgtcgaacc
    16321 caatccaatc ccggtgaaat gggcatgtaa ggaactgggt cttgtggcga ccgatacgct
    16381 gcgcctgcca atgacaccaa ttaccgacag tggtcgtgag acggtcagag cggcgcttaa
    16441 gcatgccggt ttgctgtaaa gtttagggag atttgatggc ttactctgtt caaaagtcgc
    16501 gcctggcaaa ggttgcgggt gtttcgcttg ttttattact cgctgcctgt agttctgact
    16561 cacgctataa gcgtcaggtc agtggtgatg aagcctacct ggaagcggca ccgcttgcgg
    16621 agcttcatgc cccggctgga atgatcttgc cggtgacctc cggcgattat gcaatcccgg
    16681 tgaccaacgg tagtggtgcc gtcggtaagg cgctggacat tcgtccacca gcccagccgc
    16741 tggcactggt ttctggcgcg cgtacccagt tcacgggcga taccgcttca ttgctggtgg
    16801 aaaatggtcg tggcaatact ctgtggccgc aggtggttag cgtgctgcag gcgaaaaact
    16861 acaccatcac ccaacgtgat gatgctggtc agacactgac caccgattgg gtacaatgga
    16921 accgtctgga cgaagacgag cagtatcgtg gtcgttatca aatctctgtt aagccgcagg
    16981 gttatcagca ggcggttacg gttaaactgc tgaacctgga acaggcgggc aaaccggttg
    17041 cagacgcggc gtccatgcag cgttacagca cggagatgat gaacgttatt tccgccggtc
    17101 tggataaatc tgccactgac gccgcgaacg ctgcgcaaaa tcgtgcctcc accactatgg
    17161 acgtacaaag tgcagctgat gacaccggtt taccaatgct ggtcgtacgc gggccgttca
    17221 atgtggtttg gcaacgtctg ccagctgcgc tggaaaaagt gggcatgaaa gtgaccgaca
    17281 gcacccgttc gcagggcaac atggccgtaa cttataagcc gctgtctgac agcgaatggc
    17341 acgaactggg cgcgagcgat ccaggtctgg catccggtga ctataaactg caggttggcg
    17401 atttagataa ccgcagcagc ctacagttca tcgatccgaa aggtcatact ctgactcaga
    17461 gtcagaacga cgcgctggta gctgtcttcc aggctgcgtt tagcaagtaa aaatacaggg
    17521 ctggagtcat ccggcccttt tttctgatat gatacgcaaa cgtgtgcgtc gacagaaaaa
    17581 cgcgatttta gcggtaattc gcacgaaatt tgtttgtcgg acgtaggtcg gataaggcgt
    17641 ttacgccgca tccgacaaaa catccggcac accagacagc aaaagatttt aaaacgttaa
    17701 ttcacaccca ggagtgataa agatgcaaaa gcaagctgag ttgtatcgtg gtaaagcgaa
    17761 aaccgtatac agcacggaaa acccggacct gttggtgctc gaattccgca atgatacgtc
    17821 agcaggggat ggcgcgcgca ttgagcagtt tgatcgcaaa ggtatggtga acaacaagtt
    17881 caactacttc attatgagca aactggctga agcgggtatc ccgactcaaa tggagcgtct
    17941 gctctccgat accgaatgtc tggtgaaaaa gctggatatg gtgccggttg agtgtgtcgt
    18001 gcgtaaccgt gctgctggct ctctggtgaa acgtcttgga atcgaagaag gtattgagct
    18061 gaacccgcca ctgttcgatc tgttcctgaa aaacgacgcc atgcacgatc cgatggtcaa
    18121 cgaatcttac tgcgaaacct ttggctgggt gagcaaagag aatctggcgc gtatgaaaga
    18181 gctgacctac aaagcgaacg acgtgctgaa aaaactgttc gatgatgcag gtctgattct
    18241 ggtcgacttc aagctggagt ttggtctgta caaaggcgaa gtggttctgg gcgatgagtt
    18301 ctccccggac ggcagccgcc tgtgggacaa agaaacgctg gagaaaatgg ataaagaccg
    18361 tttccgccag agcctcggtg gcctgatcga agcctatgaa gccgttgccc gccgcctggg
    18421 tgtacagctg gactgatttt tctattcatc atcttgccgt gctactggca cggcaagaca
    18481 accgctctca taagatgagt attgaacgct cattcctccg ccatttcatc ccgctttcct
    18541 gtggtaatct tcctctgaac cacctacgat catttctata atgaatataa tgttgaggta
    18601 tctatgcgtt ggcaagggcg acgtgaaagt gacaatgttg aagacaggcg caatagctcc
    18661 ggcggtccgt caatgggcgg tcccggtttt cgcctgccaa gcggtaaagg cgggctgatt
    18721 ttactgatag tcgtgctggt tgcaggctac tatggcgttg atttaaccgg gttgatgacc
    18781 ggacagccgg tttcccaaca gcaatcaacg cggtcaatta gccctaatga agacgaagcc
    18841 gcaaaattca cctcggtgat tctggcaacc acggaagaca cctggggaca gcaattcgag
    18901 aagatgggta aaacctatca gcaaccgaag ctggtcatgt accgtggaat gacgcgtacc
    18961 ggctgcgggg cgggccagtc cataatgggg ccattctatt gcccggcgga tggcacagtt
    19021 tatatcgatc tctccttcta tgatgacatg aaagacaaac ttggcgcgga tggcgatttt
    19081 gcccaggggt acgttatcgc tcatgaagtc ggtcatcatg tgcagaaact gttaggcatc
    19141 gagccgaaag ttcgtcaact gcaacaaaac gcgacgcagg cggaagtaaa ccgcttatct
    19201 gtgcgtatgg aactccaggc cgactgtttt gccggtgtct gggggcatag tatgcagcag
    19261 caaggtgttc tggaaaccgg cgatctggaa gaggcgctga acgcggcgca ggccatcggc
    19321 gatgaccgtt tacaacagca aagtcagggg cgagtagtac cagacagttt cactcatggc
    19381 acttctcagc aacgctacag ctggtttaaa cgtggtttcg acagcggcga tccggcacaa
    19441 tgcaatactt ttggtaaaag catttaactt ccggggcagg gatggctgaa ctgactgcgc
    19501 ttcacacatt aacagcgcaa atgaaacgtg aagggatccg ccgcttgctg gtgttgagcg
    19561 gggaagaggg ttggtgtttt gatcatgcgc ttaagttgcg tgatgcctta cctggcgact
    19621 ggctgtggat ttcgccgcag ccagatgctg aaaaccactg ttcaccctcg gcgctacaaa
    19681 ctttacttgg gcgcgagttc cggcatgcgg tattcgacgc ccgccacggc tttgatgccg
    19741 ctgcctttgc ggcacttagc ggaacgttga aagcgggaag ttggctggtt ttgttactcc
    19801 ctgtatggga agagtgggaa aaccaacctg atgccgactc gctacgctgg agtgattgcc
    19861 ctgaccctat tgcgacgccg cattttgtcc agcatctcaa acgcgtactt acggcggata
    19921 acgacgctat tctctggcgg caaaaccagc cgttctcgtt ggcgcatttt actccccgta
    19981 ctgactggca ccccgcgacc ggcacaccac aaccagaaca acagcaactc ttacagcagc
    20041 tactgaccat gccattgggc gtggcggtgg taacggctgc gcgtgggcgc ggtaaatcgg
    20101 cgctggcagg gcaactcatt tctcgtattg cgggtagtgc gattgtcacc gcgcccgcaa
    20161 aagcggcaac ggatgtactg gcacaatttg cgggcgagaa gtttcgcttt attgcgccgg
    20221 atgccttgtt agccagcgat gagcaagccg actggctggt ggtcgatgaa gccgcagcca
    20281 tacctgcgcc gttgttgcat caactggtat cgcgttttcc tcgaacgttg ttaaccacta
    20341 cggtgcaggg ctacgaaggt acaggacgtg gttttttgct gaaattttgc gctcgctttc
    20401 cgcatttaca ccgttttgaa ctgcaacagc cgatccgctg ggcgcagaga tgcccgctgg
    20461 aaaaaatggt tagtgaggca ctggtgtttg acgatgaaaa cttcacccat acaccacaag
    20521 gcaatatcgt catttccgca tttgaacaga cgttatggcg aagtgagcca gaaacgccgt
    20581 tgaaggttta tcagttattg tctggtgcgc actaccggac ttcgccgctg gatttacgcc
    20641 gcatgatgga tgcaccaggt caacattttt tacaggcggc tggcgaaaac gagattgccg
    20701 gagcgctgtg gctggtggat gagggtggat tatctcaaga actcagtcag gcggtatggg
    20761 cgggttatcg tcgtccacgg ggcaatctgg tggctcaatc gctggcggcg cacggcagca
    20821 atccactggc ggcgacattg cgcggacggc gggttagccg gatagcagtt catccggctc
    20881 gtcagcggga aggcacaggg cgacagctta ttgctggtgc tttgcaatat attcatgacc
    20941 tcgactatct ttcggtgagt tttggttaca ccgaggagtt atggcgtttc tggcaacgct
    21001 gcggttttgt gctggtgcga atgggtaatc atcgtgaagc cagcagcggt tgctatacgg
    21061 cgatggcgct gttaccgatg agtgatgcgg gtaaacaact ggctgaacgt gagcattacc
    21121 gcttacgtcg cgatgcgcaa gctctcgcgc agtggaatgg cgaaatgctt cctgttgatc
    21181 cactaaacga tgccgtcctt tctgacgacg actggcttga actggccggt tttgctttca
    21241 ctcatcgtcc gctattaaca tcgttaggtt gcttattgcg tctgctacaa accagcgaac
    21301 tggcattacc ggcgctgcgt gggcgtttac agaaaaacgc cagcgacgcg cagttatgta
    21361 ccacacttaa actttcaggc cgtaagctgt tactggttcg ccagcgggaa gaggcagcgc
    21421 aggcgctatt cgcacttgat gatgttcgca ctgagcgtct gcgcgatcgc ataacgcaat
    21481 ggcaattttt tcactgactc cttcaatttt ctcccatggt cattgtgagt cagcggcgta
    21541 aaattaatga catcagttaa ggagttcgcc atgaaacatg accattttgt tgttcaaagc
    21601 ccggataaac cagcacaaca gttgttactg cttttccacg gtgtcgggga taacccggtg
    21661 gcaatggggg aaataggtag ctggtttgcg ccgctgtttc ctgatgcgct ggtggtgagt
    21721 gtcggtggcg cggagccgag cggtaatccg gcggggcgtc agtggttttc ggtgcagggt
    21781 attacggaag ataatcgcca ggcgcgtgtg gatgcgatca tgccgacgtt tattgagacg
    21841 gtgcgctact ggcagaaaca gagcggggta ggggctaacg ccacggcgct gatcggtttc
    21901 tcacaaggcg cgattatggc gctggagagc attaaagccg aaccgggcct tgcttcgcgc
    21961 gtcattgcct tcaacggacg ctacgcgagc ctgccggaaa ccgcgtctac agccacgaca
    22021 attcacttga ttcatggcgg tgaagatccg gttatcgatc tggcgcatgc ggttgcggcg
    22081 caggaagcgt taatcagtgc cggtggtgac gtgacgctgg atatcgtgga ggatttaggc
    22141 catgcgatcg acaatcgcag tatgcaattt gccctggatc acttgcgtta taccattccg
    22201 aaacattatt tcgacgaagc gctaagcggc ggtaagcctg gcgatgatga tgtgattgaa
    22261 atgatgtaat aatagccggg taggcctgat aagactgtat caggcatcat ggccggatgc
    22321 ggcgttaacg ccttatccgg ccacttagct tacttcttcg gttgatcctt tttcggccag
    22381 tcgtcgtcat cgtcccactt atcgttgaaa tcacgatgcg ggggaagctc cggcttgttg
    22441 gcgagaaatt tcttatggtc gacgcgcttg agatctttaa tcacgttcag caggacgcct
    22501 accaaaaaga caatcaccag aatccaccaa tatttagcca gccagtccat gcttatttcc
    22561 tcttgcagaa ccactcatca ggcgacgagc tgttccatga tacgttgata catacgggca
    22621 agtagctgca ggtcggcagc gttcacacat tcattaattt tatgaatcgt ggcattgacc
    22681 ggcccgagtt ccaccacctg cgcccccatg cgggcaataa agcgcccgtc ggacgttccg
    22741 cctgtggtca gtagctgcgg tttaatttca ttatagtgct caaccgcgtt aacgaccgca
    22801 tccaccagtt taccgcgcgc ggtcaaaaat ggctgcccgg aaagccacca atccaccgta
    22861 tagcgcagtt gatgtttttc aagcagggca agcacctgcg ctttgatcat ctcatccgtc
    22921 agttcggtgc taaagcggaa gttaaactgc acaaacagtt cacctggaat aacgttgtta
    22981 ctgcccgttc ccgcctgaat attggcaatc tgcatactgg tcgccgggaa gaattcatta
    23041 ccctgatccc actcaatagc cactaattca ttaatgaaag gtgctgcgcg atgtaccgga
    23101 ttgtcagcca gatgtgggta ggcaacatgc ccctgaacgc catgaatggt aaggttgcag
    23161 gttaacgatc cgcgacgacc atttttcacc acatcaccta ccacttcgat actcgacggt
    23221 tcgccaacca gacagtaatc gagacgttca ttacgcgcca ttaacgcttc gacgactttt
    23281 accgtaccgt tgtgggcact agcttcttca tcagaggtga tcagaaatgc cagtcgcccc
    23341 gtatggttgg gatgttgtgc gacaaagcgt tctgccgcta ccaccatcgc cgccagcgaa
    23401 cctttcatat ctgccgcacc gcgcccgaat aacatgccgt cacgaatggt tggttcaaac
    23461 ggcggattga tccaacggtc ggcgtcgcca ggcggcacca cgtcggtatg cccggcaaag
    23521 gctaacgttt caccctgccc acgccatgcc caaaaattct gcgtgtcggc aaagtccatg
    23581 cgttcaacgg taaaaccgat cgcctgcaaa cgttcaatca acaaagcctg gcatcctgcg
    23641 tcatcaggac tcagggaagg gcggcgaata agctgttgtg tcagctcaat aaccgggcac
    23701 gacatagact acacctcatg gaaaaattgc tgataactgg attcactgaa acccagcagc
    23761 ataggcttac caggcgcgca gagcaatgga cgtttgataa ttgcaggcat ttcagtcatt
    23821 aatgtcgccg cagaggccgc atcggtgatt ttattgcggg tggtttcgtc cagtttacgc
    23881 caggttgtac cacgggtgtt gagtaacgct tcccagccta attcgttgat aaaaccgttc
    23941 aataattcgc tgtccaatcc atcgacgcgg taatcatgaa aacgatagtc gatgttattg
    24001 gcttccagcc aacggcgagc ctttttaatg gtgtcacaat ttttgatgcc gtaaagtgta
    24061 accataatga atcctttttc gatttatgag aataaatact catttaaggg tgaaaacatt
    24121 ttgcctgcaa atgatcatca ggaaatataa cattatcgaa gt
//
LOCUS       DADSYM010000069        23293 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2415
            SAMN08039579-rid11723173.denovo.069, whole genome shotgun sequence.
ACCESSION   DADSYM010000069 DADSYM010000000
VERSION     DADSYM010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039579
            Sequence Read Archive: SRR6362533
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 23293)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23293)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980162.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 03:39:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,484
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 4,347
            CDSs (with protein)               :: 4,347
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 43 of 85
            Pseudo Genes (incomplete)         :: 37 of 85
            Pseudo Genes (internal stop)      :: 19 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23293
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039579-rid11723173.denovo.069"
                     /strain="LGE2415"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8302 N 95.9628 W"
                     /collection_date="2015-08-24"
     gene            137..1342
                     /gene="gltS"
                     /locus_tag="J1Y83_002345"
     CDS             137..1342
                     /gene="gltS"
                     /locus_tag="J1Y83_002345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/glutamate symporter"
                     /protein_id="HBA7630490.1"
                     /translation="MFHLDTLATLVAATLTLLLGRKLVHSVSFLKKYTIPEPVAGGLL
                     VALALLILKKSMGWEVNFDMSLRDPLMLAFFATIGLNANLASLRAGGRVVGVFLIVVV
                     GLLVMQNAIGIGMASLLGLDPLMGLLAGSITLSGGHGTGAAWSKLFIERYGFTNATEV
                     AMACATFGLVLGGLIGGPVARYLVKHSTTPNGIPDDQEVPTAFEKPDVGRMITSLVLI
                     ETIALIAICLTVGKIVAQLLAGTAFELPTFVCVLFVGVILSNGLSMMGFYRVFERAVS
                     VLGNVSLSLFLAMALMGLKLWELASLALPMLAILVVQTIFMALYAIFVTWRMMGKNYD
                     AAVLAAGHCGFGLGATPTAIANMQAITERFGPSHMAFLVVPMVGAFFIDIVNALVIKL
                     YLMLPIFAG"
     gene            1345..2211
                     /locus_tag="J1Y83_002346"
     CDS             1345..2211
                     /locus_tag="J1Y83_002346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312555.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA7630491.1"
                     /translation="MKKRRKEPETLREHCRHIFGDEPPVLCVWETEFDYADAELKALA
                     AKEWQQIGEWDLSAYYVLNLVYNEPMQIELFRYLFPLCLAQWHETVLAGGYGDHFEES
                     LMKALRRPYLWQEMMNASQRQQVRQFLLETALQRMDNERGFNNVLCWLAVFNTLGGAA
                     PLIHSLWSRWWVLDTPGKAVCAIQYAAHLIYPIEANPLWSQEWLGWGHPLGHKDGWSS
                     ENRAFLRQMLTPEMIVAGIQAAAEILRGEPEGAMAARIAQDAYEAVDIITIQIEDLLR
                     DLSCDESGHALE"
     gene            complement(2196..4277)
                     /gene="recG"
                     /locus_tag="J1Y83_002347"
     CDS             complement(2196..4277)
                     /gene="recG"
                     /locus_tag="J1Y83_002347"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HBA7630492.1"
                     /translation="MKGRLLDAVPLSSLTGVGAALSNKLAKINLHTVQDLLLHLPLRY
                     EDRTHLYPIGELLPGVYATVEGEVLNCNISFGGRRMMTCQISDGSGILTMRFFNFNAA
                     MKNSLATGRRVLAYGEAKRGKYGAEMIHPEYRVQGDLSTPELQETLTPVYPTTEGVKQ
                     ATLRKLTDQALDLLDTCAIEELLPPELSQGMMTLPEALRTLHRPPPTLQLSDLETGQH
                     PAQRRLILEELLAHNLSMLALRAGAQRFHAQPLSANDALKNKLLAALPFKPTGAQARV
                     VAEIERDMALDVPMMRLVQGDVGSGKTLVAALAALRAVAHGKQVALMAPTELLAEQHA
                     NNFRNWFAPLGIEVGWLAGKQKGKARLAQQEAIASGQVQMIVGTHAIFQEQVQFNGLA
                     LVIIDEQHRFGVHQRLALWEKGQQQGFHPHQLIMTATPIPRTLAMTAYADLDTSVIDE
                     LPPGRTPVTTVAIPDTRRTDIIDRVRHACMTEGRQAYWVCTLIEESELLEAQAAEATW
                     EELKLALPELNVGLIHGRMKPADKQAVMASFKQGELHLLVATTVIEVGVDVPNASLMI
                     IENPERLGLAQLHQLRGRVGRGAVASHCVLLYKTPLSKTAQIRLQVLRDSNDGFVIAQ
                     KDLEIRGPGELLGTRQTGNAEFKVADLLRDQAMIPEVQRLARHIHERYPQQAKALIER
                     WMPETERYSNA"
     gene            complement(4283..4972)
                     /gene="trmH"
                     /locus_tag="J1Y83_002348"
     CDS             complement(4283..4972)
                     /gene="trmH"
                     /locus_tag="J1Y83_002348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(18)-2'-O)-methyltransferase
                     TrmH"
                     /protein_id="HBA7630493.1"
                     /translation="MNPTRYARICEMLARRQPDLTVCMEQVHKPHNVSAIIRTADAVG
                     VHEVHAVWPGSRMRTMASAAAGSNSWVQVKTHRTIGDAVAHLKGQGMQILATHLSDNA
                     VDFREIDYTRPTCILMGQEKTGITQEALALADQDIIIPMIGMVQSLNVSVASALILYE
                     AQRQRQNAGMYLRENSMLPEAEQQRLLFEGGYPVLAKVAKRKGLPYPHVNQQGEIEAD
                     ADWWSTMQAAG"
     gene            complement(4979..7087)
                     /gene="spoT"
                     /locus_tag="J1Y83_002349"
     CDS             complement(4979..7087)
                     /gene="spoT"
                     /locus_tag="J1Y83_002349"
                     /EC_number="3.1.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional GTP
                     diphosphokinase/guanosine-3',5'-bis pyrophosphate
                     3'-pyrophosphohydrolase"
                     /protein_id="HBA7630494.1"
                     /translation="MYLFESLNQLIQNYLPEDQIKRLRQAYLVARDAHEGQTRSSGEP
                     YITHPVAVACILAEMKLDYETLMAALLHDVIEDTPATYQDMEQLFGKSVAELVEGVSK
                     LDKLKFRDKKEAQAENFRKMIMAMVQDIRVILIKLADRTHNMRTLGSLRPDKRRRIAR
                     ETLEIYSPLAHRLGIHHIKTELEELGFEALYPNRYRVIKEVVKAARGNRKEMIQKILS
                     EIEGRLQEAGIPCRVSGREKHLYSIYCKMVLKEQRFHSIMDIYAFRVIVNDSDTCYRV
                     LGQMHSLYKPRPGRVKDYIAIPKANGYQSLHTSMIGPHGVPVEVQIRTEDMDQMAEMG
                     VAAHWAYKEHGETSTTAQIRAQRWMQSLLELQQSAGSSFEFIESVKSDLFPDEIYVFT
                     PEGRIVELPAGATPVDFAYAVHTDIGHACVGARVDRQPYPLSQPLTSGQTVEIITAPG
                     ARPNAAWLNFVVSSKARAKIRQLLKNLKRDDSVSLGRRLLNHALGGSRKLNEIPQENI
                     QRELDRMKLATLDDLLAEIGLGNAMSVVVAKNLQHGDASIPPATQSHGHLPIKGADGV
                     LITFAKCCRPIPGDPIIAHVSPGKGLVIHHESCRNIRGYQKEPEKFMAVEWDKETAQE
                     FITEIKVEMFNHQGALANLTAAINTTTSNIQSLNTEEKDGRVYSAFIRLTARDRVHLA
                     NIMRKIRVMPDVIKVTRNRN"
     gene            complement(7106..7381)
                     /gene="rpoZ"
                     /locus_tag="J1Y83_002350"
     CDS             complement(7106..7381)
                     /gene="rpoZ"
                     /locus_tag="J1Y83_002350"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013511051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HBA7630495.1"
                     /translation="MARVTVQDAVEKIGNRFDLVLVAARRARQMQVGGKDPLVPEEND
                     KTTVIALREIEEGLINNQILDVRERQEQQEQEAAELQAVTAIAEGRR"
     gene            complement(7436..8059)
                     /gene="gmk"
                     /locus_tag="J1Y83_002351"
     CDS             complement(7436..8059)
                     /gene="gmk"
                     /locus_tag="J1Y83_002351"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HBA7630496.1"
                     /translation="MAQGTLYIVSAPSGAGKSSLIQALLKTQPLYDTQVSVSHTTRQP
                     RPGEVHGEHYFFVNHDEFKEMISRDAFLEHAEVFGNYYGTSREAIEQVLATGVDVFLD
                     IDWQGAQQIRQKMPHARSIFILPPSKIELDRRLRGRGQDSEEVIAKRMAQAVAEMSHY
                     AEYDYLIVNDDFDTALTDLKTIIRAERLRMSRQKQRHDALISKLLAD"
     gene            8317..9999
                     /locus_tag="J1Y83_002352"
     CDS             8317..9999
                     /locus_tag="J1Y83_002352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709426.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA ligase B"
                     /protein_id="HBA7630497.1"
                     /translation="MKVWMAILISILCWQSSAWAVCPAWSPARAQEEISRLQQQIKQW
                     DDDYWKEGKSEVEDGVYDQLSARLTQWQRCFGNETRDVMMPPLNGAVMHPVAHTGVRK
                     MADKNALSLWMRERSDLWVQPKVDGVAVTLVYRDGKLNKAISRGNGLKGEDWTQKVRL
                     ISAVPQTVSGPLANSTLQGEIFLQRKGHIQQQMGGINARAKVAGLMMRQGNSDTLNSL
                     AVFVWAWPDGPHLMTDRLKDLATAGFTLTQTYTRAVKNADEVAHVRNEWWKAKLPFVT
                     DGVVVRAAKEPESRHWLPGQAEWLVAWKYQPVAQVAEVKAIQFAVGKSGKISVVASLA
                     PVMLDDKKIQRVNIGSVRRWQEWDIAPGDQILVSLAGQGIPRIDDVVWRGAERTKPTP
                     PENRFNSLTCYFASDVCQEQFISRLVWLGSKQVLGLDGIGEAGWRALHQTHRFEHIFS
                     WLLLTPEQLQNTPGIAKSKSAQLWHQFNLARQQPFTRWVMAMGIPLTRAALNASDERS
                     WSQLLFSTEQFWQQLPGTGSGRARQVIEWKENAQIKKLGSWLAAQQITGFEP"
     gene            complement(9996..10613)
                     /locus_tag="J1Y83_002353"
     CDS             complement(9996..10613)
                     /locus_tag="J1Y83_002353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312548.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimeric intracellular cation channel family
                     protein"
                     /protein_id="HBA7630498.1"
                     /translation="MLLHILYLVGITAEAMTGALAAGRRRMDTFGVIIIATATAIGGG
                     SVRDILLGHYPLGWVKHPEYVIIVATAAVLTTIVAPVMPYLRKVFLVLDALGLVVFSI
                     IGAQVALDMGHGPIIAVVAAVTTGVFGGVLRDMFCKRIPLVFQKELYAGVSFASAVLY
                     IALQHYVSNHDVVIISTLVFGFFARLLALRLKLGLPVFYYSHEGH"
     gene            complement(10865..11728)
                     /locus_tag="J1Y83_002354"
     CDS             complement(10865..11728)
                     /locus_tag="J1Y83_002354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HBA7630499.1"
                     /translation="MIRSMTAYARREIKGEWGSATWEMRSVNQRYLETYFRLPEQFRS
                     LEPVVRERIRSRLTRGKVECTLRYEPDVSAQGELILNEKLAKQLVTAANWVKMQSDEG
                     EINPVDILRWPGVMAAQEQDLDAIAAEILAALDGTLDDFIIARETEGQALKVLIEQRL
                     EGVTAEVAKVRAHMPEILQWQRERLVAKLEDAQVQLENNRLEQELVLLAQRIDVAEEL
                     DRLEAHVKETYNILKKKEAVGRRLDFMMQEFNRESNTLASKSINAEVTNSAIELKVLI
                     EQMREQIQNIE"
     gene            11855..12571
                     /gene="rph"
                     /locus_tag="J1Y83_002355"
     CDS             11855..12571
                     /gene="rph"
                     /locus_tag="J1Y83_002355"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006803115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HBA7630500.1"
                     /translation="MRPAGRSNNQVRPVTLTRNYTKHAEGSVLVEFGDTKVLCTASIE
                     EGVPRFLKGQGQGWITAEYGMLPRSTHTRNAREAAKGKQGGRTMEIQRLIARALRAAV
                     DLKALGEFTITLDCDVLQADGGTRTASITGACVALADALQKLVENGKLKTNPMKGMVA
                     AVSVGIVNGEAVCDLEYVEDSAAETDMNVVMTEDGRIIEVQGTAEGEPFTHEELLTLL
                     ALARGGIESIVATQKAALAN"
     gene            12637..13278
                     /gene="pyrE"
                     /locus_tag="J1Y83_002356"
     CDS             12637..13278
                     /gene="pyrE"
                     /locus_tag="J1Y83_002356"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HBA7630501.1"
                     /translation="MKPYQRQFIEFALSKQVLKFGEFTLKSGRKSPYFFNAGLFNTGR
                     DLALLGRFYAEALVDSGIEFDLLFGPAYKGIPIATTTAVALAEHHDLDLPYCFNRKEA
                     KDHGEGGNLVGSALQGRVMLVDDVITAGTAIRESMEIIQANGATLAGVLISLDRQERG
                     RGEISAIQEVERDYNCKVISIITLKDLIAYLEEKPEMAEHLAAVKAYREEFGV"
     gene            complement(13315..13911)
                     /gene="slmA"
                     /locus_tag="J1Y83_002357"
     CDS             complement(13315..13911)
                     /gene="slmA"
                     /locus_tag="J1Y83_002357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000818604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid occlusion factor SlmA"
                     /protein_id="HBA7630502.1"
                     /translation="MAEKQTAKRNRREEILQSLALMLESSDGSQRITTAKLAASVGVS
                     EAALYRHFPSKTRMFDSLIEFIEDSLITRINLILKDEKDTTARLRLIVLLLLGFGERN
                     PGLTRILTGHALMFEQDRLQGRINQLFERIEAQLRQVLREKRMREGEGYTTDETLLAS
                     QILAFCEGMLSRFVRSEFKYRPTDDFDARWPLIAAQLQ"
     gene            complement(14018..14476)
                     /gene="dut"
                     /locus_tag="J1Y83_002358"
     CDS             complement(14018..14476)
                     /gene="dut"
                     /locus_tag="J1Y83_002358"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000976070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HBA7630503.1"
                     /translation="MMKKIDVKILDPRVGKEFPLPTYATSGSAGLDLRACLDDAVELA
                     PGDTTLVPTGLAIHIADPSLAAMMLPRSGLGHKHGIVLGNLVGLIDSDYQGQLMISVW
                     NRGQDSFTIQPGERIAQMIFVPVVQAEFNLVEDFDATDRGEGGFGHSGRQ"
     gene            complement(14454..15674)
                     /gene="coaBC"
                     /locus_tag="J1Y83_002359"
     CDS             complement(14454..15674)
                     /gene="coaBC"
                     /locus_tag="J1Y83_002359"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418096.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HBA7630504.1"
                     /translation="MSLAGKKIVLGVSGGIAAYKTPELVRRLRDRGADVRVAMTEAAK
                     AFITPLSLQAVSGYPVSDSLLDPAAEAAMGHIELGKWADLVILAPATADLIARVAAGM
                     ANDLVSTICLATPAPVAVLPAMNQQMYRAAATQHNLEVLASRGLLIWGPDSGSQACGD
                     IGPGRMLDPLTIVDMAVAHFSPVNDLKHLNIMITAGPTREPLDPVRYISNHSSGKMGF
                     AIAAAAARRGANVTLVSGPASLPTPPFVNRVDVMTALEMEAAVNASVQQQNIFIGCAA
                     VADYRAATVAPEKIKKQATQGDELTIKMVKNPDIVAGVAALKDHRPYVVGFAAETNNV
                     EEYARQKRIRKNLDLICANDVSQPTQGFNSDNNALHLFWQDGDKVLPLERKELLGQLL
                     LDEIVTRYDEKNRR"
     gene            15846..16514
                     /locus_tag="J1Y83_002360"
     CDS             15846..16514
                     /locus_tag="J1Y83_002360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709417.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="JAB domain-containing protein"
                     /protein_id="HBA7630505.1"
                     /translation="MKNNVQLLMPREKMLKFGISALTDVELLALFLRTGTRGKDVLTL
                     AKEMLENFGSLYGLLTSEYEQFSGVHGIGVAKFAQLKGIAELARRYYNVRMREESPLL
                     SPEMTREFLQSQLTGEEREIFMVIFLDSQHRVITHSRLFSGTLNHVEVHPREIIREAI
                     KINASALILAHNHPSGCAEPSKADKLITERIIKSCQFMDLRVLDHIVIGRGEYVSFAE
                     RGWI"
     gene            16731..16967
                     /gene="rpmB"
                     /locus_tag="J1Y83_002361"
     CDS             16731..16967
                     /gene="rpmB"
                     /locus_tag="J1Y83_002361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008457442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HBA7630506.1"
                     /translation="MSRVCQVTGKRPVTGNNRSHALNATKRRFLPNLHSHRFWVESEK
                     RFVTLRVSAKGMRVIDKKGIDTVLAELRARGEKY"
     gene            16988..17155
                     /gene="rpmG"
                     /locus_tag="J1Y83_002362"
     CDS             16988..17155
                     /gene="rpmG"
                     /locus_tag="J1Y83_002362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010847629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HBA7630507.1"
                     /translation="MAKGIREKIKLVSSAGTGHFYTTTKNKRTKPEKLELKKFDPVVR
                     QHVIYKEAKIK"
     gene            17253..18062
                     /gene="mutM"
                     /locus_tag="J1Y83_002363"
     CDS             17253..18062
                     /gene="mutM"
                     /locus_tag="J1Y83_002363"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HBA7630508.1"
                     /translation="MPELPEVETSRRGIEPHLAGATILHAVVRNGRLRWPVSEEIYRL
                     SDQPVLSVQRRAKYLLLELPEGWIIIHLGMSGSLRILPEELPPEKHDHVDLVMSNGKV
                     LRYTDPRRFGAWLWTKELEGHNVLAHLGPEPLSDDFNGEYLHQKCAKKKTAIKPWLMD
                     NKLVVGVGNIYASESLFAAGIHPDRLASSLSLAECELLARVIKAVLLRSIEQGGTTLK
                     DFLQSDGKPGYFAQELQVYGRKGEPCRVCGTPIVATKHAQRATFYCRQCQK"
     gene            complement(18101..18580)
                     /gene="coaD"
                     /locus_tag="J1Y83_002364"
     CDS             complement(18101..18580)
                     /gene="coaD"
                     /locus_tag="J1Y83_002364"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HBA7630509.1"
                     /translation="MQKRAIYPGTFDPITNGHIDIVTRATQMFDHVILAIAASPSKKP
                     MFTLEERVELAQQATAHLGNVEVVGFSDLMANFASNQHATVLIRGLRAVADFEYEMQL
                     AHMNRHLMPELESVFLMPSKEWSFISSSLVKEVARHQGDVTHFLPENVHQALMAKLA"
     gene            complement(18588..19865)
                     /gene="waaA"
                     /locus_tag="J1Y83_002365"
     CDS             complement(18588..19865)
                     /gene="waaA"
                     /locus_tag="J1Y83_002365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-D-manno-octulosonic acid transferase"
                     /protein_id="HBA7630510.1"
                     /translation="MLELLYTALLYLIQPLIWIRLWVRGRKAPAYRKRWGERYGFYRH
                     PLKPGGIMLHSVSVGETLAAIPLVRALRHRYPDLPITVTTMTPTGSERVQSAFGKDVQ
                     HVYLPYDLPDALNRFLNKVDPKLVLIMETELWPNLIAALHKRKIPLVIANARLSARSA
                     AGYAKLGKFVRRLLRRITLIAAQNEEDGARFVALGAKNNQVTVTGSLKFDISVTPQLA
                     AKAVTLRRQWAPHRPVWIATSTHEGEESVVIAAHQALLQQFPNLLLILVPRHPERFPD
                     AINLVRQAGLSYITRSSGEVPSTSTQVVVGDTMGELMLLYGIADLAFVGGSLVERGGH
                     NPLEAAAHAIPVLMGPHTFNFKDICARLEQASGLITVTDATTLAKEVSSLLTDADYRS
                     FYGRHAVEVLYQNQGALQRLLQLLEPYLPPKTH"
     gene            20278..21336
                     /gene="rfaQ"
                     /locus_tag="J1Y83_002366"
     CDS             20278..21336
                     /gene="rfaQ"
                     /locus_tag="J1Y83_002366"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptosyltransferase
                     RfaQ"
                     /protein_id="HBA7630511.1"
                     /translation="MDKPFRRILLIKMRFHGDMLLTTPVISSLKKNYPDAKIDVLLYQ
                     DTIPILSENPEINALYGIKNKKAKASEKITNFFHLIKVLRANKYDLIVNLTDQWMVAI
                     LVRLLNARVKISQDYHHRQSAFWRNSFTHLVPLQGGNVVESNLSVLTPLGLESLVKQT
                     TMSYPPASWKRMRRELDHAGVGQNYVVIQPTARQIFKCWDNAKFSAVIDALHARGYEV
                     VLTSGPDKDDLACVNEIAQGCQTPPVTALAGKVTFPELGALIDHAQLFIGVDSAPAHI
                     AAAVNTPLISLFGATDHIFWRPWSNNMIQFWAGDYREMPTRDQRDRNEMYLSVIPAAD
                     VIAAVDKLLPSSTTGTSL"
     gene            21333..22457
                     /locus_tag="J1Y83_002367"
     CDS             21333..22457
                     /locus_tag="J1Y83_002367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HBA7630512.1"
                     /translation="MIVAFCLYKYFPFGGLQRDFMRIAQTVAARGHHVRVYTQSWEGE
                     CPDVFELIKVPVKSHTNHGRNAEYFAWVQKHLREHPVDRVVGFNKMPGLDVYYAADVC
                     YAEKVAQEKGFFYRLTSRYRHYAAFERATFEQGKPTQLLMLTDKQIADFQKHYQTEAE
                     RFHILPPGIYPDRKYSQQPANSREIFRKKNGITEQQYLLLQVGSDFTRKGVDRSIEAL
                     ASLPDSLRHNTLLYVVGQDKPRKFEALAEKRGVRSNVHFFSGRNDVSELMAAADLLLH
                     PAYQEAAGIVLLEAITAGLPVLTTAVCGYAHYIVDANCGEAIAEPFRQETLNEILRKA
                     LTQSSLRQAWAENARHYADTQDLYSLPEKAADIITGGLDG"
     gene            22450..23247
                     /gene="rfaP"
                     /locus_tag="J1Y83_002368"
     CDS             22450..23247
                     /gene="rfaP"
                     /locus_tag="J1Y83_002368"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312532.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptose(I) kinase RfaP"
                     /protein_id="HBA7630513.1"
                     /translation="MVELKEPFATLWRGKDPFEEVKTLQGEVFRELETRRTLRFEMAG
                     KSYFLKWHRGTTLKEIIKNLLSLRMPVLGADREWNAIHRLRDVGVDTMYGVAFGEKGI
                     NPLTRTSFIITEDLTPTISLEDYCADWATNSPDVRVKRMLIKRVATMVRDMHAAGINH
                     RDCYICHFLLHLPFSGKEEVLKISVIDLHRAQIRAKVPRRWRDKDLIGLYFSSMNIGL
                     TQRDIWRFMKVYFVAPLKDIIKQEQGLLSQAEEKATKIRERTIRKSL"
BASE COUNT         5491 a         5766 c         6299 g         5737 t
ORIGIN      
        1 atgccatttt tctagtttca gctaagaatt cgaaccagga aagcatgact cgatactcgt
       61 catatttcgc cttactgcaa atgcgcgatg aagtatgacg agtatgaaag agtgatgcgg
      121 acacaaagga gtaactatgt ttcatctcga tactttagca acgcttgttg ccgcaacgct
      181 gacgttgctg ctcgggcgca agttggtcca ttccgtctcc tttttgaaaa aatacaccat
      241 accggaacct gttgcgggtg gtttattagt ggcgctggcg ctgctgatac tgaaaaaaag
      301 catgggctgg gaagtcaact ttgatatgtc cctgcgcgat ccgttgatgc tggctttctt
      361 cgccaccatt ggcctgaacg ccaaccttgc cagtctgcga gctggtgggc gtgtggtggg
      421 ggttttcctg attgtggttg ttggcctgtt ggtgatgcaa aatgccattg gcattggtat
      481 ggctagcctg ttagggcttg atccgctgat ggggctgctg gccggttcta ttacgctttc
      541 gggcggtcac ggtacaggtg ctgcatggag taaattgttc attgaacgtt acggctttac
      601 taacgcgaca gaagtggcga tggcctgtgc aacgttcggt ctggtgctgg gcgggctgat
      661 cggtggcccg gtggcgcgtt atctggtgaa acactccacc acaccgaacg gtattccgga
      721 tgatcaggaa gttccgactg cgttcgagaa gccggatgtg ggccgcatga tcacctcgct
      781 ggtgctgatt gaaactatcg cgctgattgc tatctgcctg acggtgggga aaattgttgc
      841 gcaacttttg gctggtactg cttttgaact accgaccttc gtctgtgtac tatttgttgg
      901 cgtgattctg agcaacggtc tgtcaatgat gggcttttac cgcgtctttg agcgagcggt
      961 atccgtgctg ggtaacgtaa gcctgtcgtt gttcctggcg atggcgctga tggggctgaa
     1021 actgtgggag ctggcttcgc tggcgttgcc gatgctggcg attctggtgg tacagaccat
     1081 cttcatggcg ttgtatgcca tcttcgttac ctggcgcatg atgggcaaaa actacgatgc
     1141 ggcagtgctg gctgcgggtc actgtggttt tggcctcggt gcaacgccaa cggcaatcgc
     1201 caacatgcag gcgatcactg aacgttttgg cccgtcgcac atggccttcc tggtagtgcc
     1261 gatggtcggt gcgttcttta ttgatatcgt taacgcgctg gtgattaagc tgtatttgat
     1321 gttgccgatt tttgccggtt aaaaatgaaa aagcgtcgga aggaaccgga aacgctacgt
     1381 gaacactgtc gccatatttt tggtgatgag ccgccagtgc tctgtgtatg ggaaacggaa
     1441 tttgattacg cagatgccga actcaaggcg ctggcggcta aagagtggca acagataggc
     1501 gaatgggatt tgagtgctta ctatgtactc aatctcgttt ataacgaacc gatgcaaata
     1561 gagctgtttc gctatctgtt tccgctatgc ctggcgcaat ggcatgaaac cgttttagct
     1621 ggcggttatg gtgatcactt cgaagaatcc ttaatgaagg cattgcgtcg gccctatctc
     1681 tggcaggaga tgatgaatgc ttcccaacgc cagcaggtac ggcaattttt actggaaacc
     1741 gcgttgcagc gtatggataa cgagcgtggt ttcaataatg ttctttgctg gctggcggtg
     1801 ttcaatactc tgggcggggc cgcgccgtta attcattctc tctggtcacg ctggtgggtg
     1861 ctggatacgc cgggaaaagc ggtgtgtgct attcagtatg ccgcgcatct aatttatccc
     1921 atagaagcca atccgttatg gtcgcaagaa tggttaggct gggggcatcc attaggtcat
     1981 aaagatggct ggtcctctga gaatcgcgct tttcttcgcc aaatgttaac acctgaaatg
     2041 attgtggctg gcatacaggc agcggctgaa attctgcgtg gtgaaccgga aggcgcaatg
     2101 gcggcgcgaa ttgcgcagga tgcgtatgag gcagtggata tcatcactat ccagattgaa
     2161 gatttactgc gtgacttgtc ttgtgatgag tctggtcatg cgttggagta gcgttctgtt
     2221 tctggcatcc agcgttctat cagggctttt gcctgttgtg ggtaacgttc gtgaatatgt
     2281 cgcgccaggc gctgaacttc cgggatcatc gcctgatcgc gcagtaaatc cgccacttta
     2341 aattcagcat tacccgtctg acgcgtacct aacagttcac ccggtccgcg aatctctaag
     2401 tctttctgtg caatcacaaa accgtcgttg ctgtcgcgca gcacttgcag ccgaatttgc
     2461 gcggttttgg agagcggcgt tttgtagagc agcacgcagt gagaagccac cgctccacga
     2521 cctacacgcc cgcgaagctg gtgtaactgc gccagaccca gacgctcagg gttttcgata
     2581 atcatcagac tggcgttagg cacatcaaca ccgacttcaa taacggtagt ggcaaccagc
     2641 aggtgtagtt caccttgttt aaacgacgcc atcaccgcct gtttatcggc aggtttcatc
     2701 cgcccgtgta tcaggccgac gttcagttcc ggcaacgcca gtttcaactc ttcccaggta
     2761 gcttccgccg cctgcgcttc gagtaattcc gactcttcaa tcaacgtaca aacccagtac
     2821 gcctgacgcc cttcagtcat acaggcgtgg cgcacgcggt caatgatgtc ggtacggcgg
     2881 gtatcaggaa tagcgaccgt agtcactggc gtgcggcctg gcggcagctc atctatcacc
     2941 gaggtatcga gatcggcata cgcagtcatt gccagcgtgc gggggatcgg cgtggcggtc
     3001 atgatcaact gatgaggatg gaagccttgc tgctggcctt tctcccataa cgccagacgc
     3061 tgatgcacgc caaaacgatg ctgttcgtcg ataatcacca gcgccaggcc gttaaactgc
     3121 acctgttcct ggaaaatggc gtgagtgcca acaatcatct gcacctgacc gctggcgatg
     3181 gcttcttgct gagccagtcg tgctttacct ttctgttttc cggcgagcca gcctacttcg
     3241 ataccgagcg gtgcaaacca gttacggaag ttattggcgt gctgctcggc gagtaattcg
     3301 gttggtgcca tcagtgccac ctgtttgccg tgggcaaccg cacgcaacgc ggcgagggcg
     3361 gcgaccagcg ttttaccgga acctacatcg ccctgcacca gacgcatcat cggcacatcc
     3421 agcgccatat cgcgctcgat ctccgccact acgcgtgcct gtgcgcccgt tggcttgaac
     3481 ggtaaggcgg cgaggagttt atttttcagc gcgtcattgg cgctcagcgg ctgagcatga
     3541 aaacgttgtg caccggcacg taacgccaac atgctgaggt tgtgcgccag caactcttcg
     3601 agaatcagcc ggcgttgtgc cggatgctgc ccggtttcga gatcgctaag ctgtagcgtc
     3661 ggcggcgggc ggtgcaaagt gcgcaacgct tccggtagcg tcatcattcc ttgtgacagt
     3721 tccggcggca ggagttcttc aatggcgcag gtgtcgagca gatccagcgc ctggtcggtt
     3781 aatttacgca gcgtggcctg ctttacgcct tccgtagttg ggtaaaccgg cgtgagcgtt
     3841 tcctgtaact ctggcgtgct gagatcgccc tgtacgcggt attccgggtg gatcatctcc
     3901 gcaccatatt tgccgcgctt tgcttcgcca taggccagca cgcggcggcc tgtcgccagg
     3961 ctatttttca ttgccgcatt gaagttgaaa aagcgcatag tgagaatgcc ggaaccgtcg
     4021 ctgatctggc aggtcatcat ccgccgaccg ccgaaggaga tattgcagtt cagcacttcg
     4081 ccttccaccg tggcataaac acccggcagt agttcaccga tggggtagag atgtgtgcga
     4141 tcttcgtagc gcagaggaag gtgtaagagc aaatcctgta cggtatgcag gttgattttc
     4201 gccagcttgt tactaagcgc tgcgccaacg cccgttaggg aactgagcgg gacagcatct
     4261 aacaggcgac ctttcatcgc gcttaccctg cggcctgcat ggtggaccac cagtcagcat
     4321 cggcttcaat ctcgccttgc tgattaacgt gggggtaggg cagacctttg cgttttgcga
     4381 ctttcgccag caccggatag ccgccttcaa acagcaggcg ttgttgctcg gcttccggca
     4441 acatgctgtt ttcacgcagg tacatgcccg cattttgccg ctggcgctgg gcttcgtaaa
     4501 gaatgagggc tgaggcaacg gaaacattca gcgactgcac catgccgatc atcggaatga
     4561 tgatatcctg atccgccagg gccaacgctt cctgcgtgat gcccgttttc tcctgtccca
     4621 tcaaaataca ggtcgggcga gtgtaatcaa tttcgcggaa atcaacagcg ttatcagaaa
     4681 gatgggttgc cagaatctgc atgccctggc ctttcagatg agcgacggca tcgccaatgg
     4741 tgcggtgcgt tttcacctgt acccagctgt tactacccgc agctgccgaa gccatggtgc
     4801 gcatccggct gccaggccag acagcatgaa cttcatgtac gccaacggca tctgcggtac
     4861 gaataatcgc agaaacgtta tgaggtttgt ggacctgctc catgcagacg gtcagatcag
     4921 gctgccgcct ggcgagcatt tcgcagatgc gtgcataacg tgttgggttc ataaaacatt
     4981 aatttcggtt tcgggtgact ttaatcacgt ctggcatcac gcggattttg cgcatgatat
     5041 tcgccagatg cacacggtca cgggcggtca gacgaataaa ggcgctatag acgcgaccat
     5101 ctttctcttc cgtattcaaa ctttgaatat tcgaggtcgt ggtgttaatt gccgccgtca
     5161 ggtttgccag cgcgccctga tgattgaaca tctccacctt gatttcggtg atgaattcct
     5221 gcgccgtctc tttatcccat tcaacagcca taaacttctc tggctctttc tggtagccac
     5281 ggatgttacg gcaggattca tggtggatca ccagaccttt accggggctg acgtgggcga
     5341 taatcgggtc gccaggaatt gggcggcagc atttcgcaaa ggtgatcagc acgccatcgg
     5401 cacctttaat gggcagatgt ccgtgacttt gggttgccgg tggaatggag gcgtccccat
     5461 gttgcagatt tttcgcgacc accacgctca ttgcgttacc aaggccgatt tctgccagca
     5521 gatcgtcaag cgttgccagc ttcatgcggt ccagctcgcg ctgaatattt tcctgcggga
     5581 tttcattgag cttacggcta ccacccaacg catggttgag cagacgacgg cccaggctta
     5641 cagaatcatc acgcttgagg tttttcagca actgacgaat tttggcgcgc gctttcgagc
     5701 tgacgacaaa gttcagccac gcggcattcg ggcgagcacc cggtgcggta atgatttcaa
     5761 cggtttgacc gctagtaagc ggctgcgaca gcgggtaagg ctggcggtcg acgcgtgcgc
     5821 ccacgcaggc atggccgata tcggtatgca ccgcataagc gaagtcgacg ggcgttgcac
     5881 cggcaggcag ttcgacaatg cgcccttccg gtgtgaaaac gtaaatctca tccgggaaga
     5941 gatcggattt aacgctctcg ataaattcaa acgagctacc ggcactctgt tgcagctcca
     6001 gcaggctttg catccagcgc tgggcgcgta tttgcgcggt agtgctggtt tcgccgtgct
     6061 ctttataagc ccagtgcgcg gcaacaccca tctccgccat ctgatccata tcttcggtac
     6121 ggatctggac ctcaaccggc acgccgtgcg ggccaatcat cgaggtgtgc aaagactgat
     6181 agccgttcgc ttttggaatg gcgatatagt ctttcacgcg gcccggacgc ggcttgtaca
     6241 ggctgtgcat ctggcccagc acgcgataac aggtgtcaga atcattgacg atcacgcgga
     6301 aagcgtagat gtccatgatt gagtgaaaac gctgctcttt gagcaccatt ttgcagtaaa
     6361 tcgaataaag atgcttttcg cgaccactga cgcggcacgg tattcccgct tcctgcaaac
     6421 gcccttcgat ttcagaaaga atcttctgga tcatctcttt acggttgccg cgcgcggctt
     6481 tcaccacttc tttgattacg cgataacggt tgggatacag cgcctcaaaa cccagctcct
     6541 cgagttcggt tttaatgtgg tggataccta aacggtgcgc cagcgggctg tatatttcga
     6601 gagtttcacg ggcgatgcgg cgacgtttgt ccgggcgaag tgagcccagc gtgcgcatgt
     6661 tgtgggtacg gtcggcaagt ttgatgagaa tgacgcggat atcctgcacc atcgccataa
     6721 tcatcttgcg aaagttttcg gcctgcgcct ctttcttatc gcggaacttg agtttatcaa
     6781 gtttcgacac cccctctacc agctcggcga cgcttttacc aaaaagctgt tccatgtcct
     6841 ggtaggtggc gggtgtatct tcaatcacat catgcagcag cgccgccatc agcgtttcat
     6901 agtcgagttt catctcggcc agaatgcagg caaccgccac cgggtgcgtg atatagggtt
     6961 caccgcttga acgtgtttgc ccctcgtgag catcacgtgc aacgagatac gcctgccgca
     7021 gacgcttgat ttggtcttcc ggcaggtagt tttgaatcag ctgattcagg ctttcaaaca
     7081 gatacaaggg cgacccgctt tgtgattaac gacgaccttc agcaatagcg gtaacggctt
     7141 gtaattcagc ggcttcctgc tcttgctgtt cctggcgttc gcgaacgtcg aggatctggt
     7201 tgttgatcag accttcttcg atttcgcgca gcgcgattac agtggtttta tcgttttctt
     7261 ccggtaccag cggatccttt ccgcctacct gcatctgacg agcgcgacgc gcggcgacca
     7321 gtaccaggtc aaaacggtta ccaattttct ctacagcgtc ctgaacagtt acgcgtgcca
     7381 tacttaaaaa gctccacagg tgaagaaatg actgggcatg atactgaaat caggttcagt
     7441 ctgccaacaa tttgctgatt aaagcgtcat gacgctgctt ttggcggctc atgcgcagac
     7501 gttcggcgcg aataatggtc ttcaaatcgg tcaacgcggt atcgaagtca tcattcacaa
     7561 tcaggtaatc atattcggcg taatggctca tttctgcaac agcttgcgcc atacgctttg
     7621 caatgacctc ttcgctgtcc tgaccgcgac cgcgtagacg gcggtccagt tcgattttag
     7681 acggcggtaa aataaagata ctccgcgcgt gcggcatctt ctggcgaatt tgctgcgcac
     7741 cctgccagtc gatatcgaga aaaacatcga caccggtcgc cagtacttgc tcaatggcct
     7801 cacgcgaagt gccatagtaa ttaccaaaaa cttctgcgtg ttcgaggaac gcatctctac
     7861 taatcatttc tttaaattca tcatgattaa caaagaaata atgttcaccg tggacttcac
     7921 caggacgcgg ttggcgtgtg gtgtgtgaaa cagaaacctg ggtgtcatac aacggttggg
     7981 tttttaataa agcctgaatc aggctggatt tacccgcgcc actgggggca gaaacaatat
     8041 aaagcgtgcc ttgagccatg agtatctttc gtatgtgatt agcgaaataa agcctacata
     8101 cgagcttatt atacacggcg cggccatgtg acgtagccct tgctcacact ttttgcgcaa
     8161 agttgttgca ttttgcgtag ctgcttccgc atttgcctgt gattcactgc aataaagacg
     8221 attctcagga agcgtcctcg cagactccgt ttttgccact cggctttgct ttcaccacgt
     8281 tgtatatctg cctgactatc tacagcggag gtgatgatga aagtatggat ggcgatatta
     8341 ataagtatct tgtgctggca atcatcggcg tgggcggtct gtccggcctg gtcgccagcc
     8401 agagcacagg aagaaatttc ccgcctgcaa cagcaaataa aacagtggga cgatgactac
     8461 tggaaagaag ggaaaagtga ggtggaagac ggtgtttacg atcagttaag cgcccgtctc
     8521 acgcagtggc aacgctgttt tgggaacgag actcgcgatg tgatgatgcc gcctttaaac
     8581 ggtgcggtta tgcatccggt tgcccatacg ggtgtacgca aaatggcgga taaaaacgca
     8641 ttaagtctgt ggatgcgcga acgtagcgat ctttgggtgc agccaaaagt tgatggcgtt
     8701 gcggtaaccc tggtttatcg ggacgggaaa ctgaacaaag caatcagtcg cggcaatggc
     8761 ctgaaaggcg aggactggac gcagaaagtt cgcttaattt ccgctgtgcc gcaaaccgtt
     8821 agcgggcctt tagccaacag cacgcttcag ggggaaatat ttctccagcg taaggggcat
     8881 atccaacaac aaatgggggg aataaatgcc cgcgcaaaag ttgctggcct gatgatgcgc
     8941 caggggaata gcgatacgct aaactcactg gctgtttttg tctgggcatg gccggatgga
     9001 ccgcatttaa tgaccgatcg tttaaaggat ctggctaccg caggttttac tttaacgcag
     9061 acgtataccc gcgcggttaa aaatgctgat gaggttgcgc acgtacgcaa tgagtggtgg
     9121 aaagcaaaat tacccttcgt caccgatggc gtggttgtgc gagcggcgaa agaacccgaa
     9181 tcccgtcatt ggttaccggg ccaggcggag tggctggtgg cctggaaata tcaacctgta
     9241 gctcaggttg ccgaagtgaa ggcgattcaa tttgcggtgg gtaagagcgg taaaatatcg
     9301 gtggtggcgt cactcgcacc tgtcatgctg gatgataaaa aaatccagcg ggtgaatatt
     9361 ggttccgtca ggcgctggca ggaatgggat attgcgcctg gtgatcagat cctcgtcagc
     9421 cttgccggtc aggggattcc tcgcattgat gatgtggtct ggcgcggagc agaacgtaca
     9481 aaaccgacac caccagaaaa ccgctttaac tcgttgacct gctactttgc ttctgatgtt
     9541 tgtcaggaac agttcatttc acgcttagtc tggctggggt caaaacaggt tctggggctt
     9601 gatggcattg gtgaggccgg ctggcgcgcc ctgcatcaga ctcatcgctt tgagcatatc
     9661 ttttcctggc ttttattaac gccagagcaa ttacagaaca cgccggggat cgcgaaaagt
     9721 aaaagtgcgc agttatggca tcagtttaat ctggctcgtc agcagccttt tactcgctgg
     9781 gtgatggcaa tgggaatacc gctaacccgg gcggcgctta atgccagtga tgaacggtcc
     9841 tggtcgcaac ttttatttag cacggaacag ttctggcagc agctgccagg gactggatcg
     9901 gggcgcgcca gacaggttat tgaatggaag gaaaatgcgc aaatcaagaa gctgggcagt
     9961 tggctggctg cccagcagat cacaggtttt gaaccttagt gaccttcgtg gctgtagtaa
    10021 aagaccggta atcccagctt aagacgtagc gccagtaagc gggcaaaaaa gccgaatacc
    10081 agggtggaga taattaccac atcatggtta gaaacatagt gttgcagtgc gatgtacagc
    10141 acggcggagg caaatgagac accggcatac aactctttct ggaataccag tggaatgcgt
    10201 ttacagaaca tatcgcgcaa aacgccgcca aacacgccgg tggttaccgc cgcgacaacg
    10261 gcaataattg ggccgtgccc catatccagt gcaacctgtg cgccgatgat agaaaagacc
    10321 accagtccga gcgcatcgag caccagaaac actttgcgca gataaggcat tacaggagca
    10381 actatagtgg tgagtactgc agcggtagcg acgataatca catattccgg gtgtttgacc
    10441 cagccgagcg gatagtggcc cagcagaata tcgcgcactg accctccgcc aattgccgtg
    10501 gcggtcgcaa taataattac gccaaatgta tccatgcgcc gtcgtccggc cgccagcgcc
    10561 cccgtcatgg cttcggcagt aatgccaacc aaatacaaaa tgtgtaacag catggttctc
    10621 tccctaaaaa tctgtctgga agggtagcga tttgtgcgca ttgtcacgat tgagattttt
    10681 taaggagaga atagattaat aatttgtagt tatgtgaaat tgctattttc tgtagtaaga
    10741 atgcattttt ctgattttgt gataagtaaa atgcattaga aaaagtatgg agtaaataaa
    10801 tatgaggacg aaactatatg cgtagcgccg ctgtaattaa caacggccta cggagatatg
    10861 tcgcttactc gatattctga atctgctcgc gcatctgctc aatcaacact ttcagctcga
    10921 tggcggagtt tgtcacttcg gcgttgatag atttcgacgc aagagtgttc gactcgcggt
    10981 tgaactcctg catcataaag tccagacgac gaccaacggc ttcttttttc ttcagaatgt
    11041 tgtaggtctc tttgacatgc gcttcgaggc ggtccagttc ttcggcaacg tcaattcgtt
    11101 gtgccagcag aaccaactcc tgctccagac ggttgttttc cagttgcacc tgagcatctt
    11161 ccagcttcgc taccagacgc tcacgctgcc attgcaggat ttccggcata tgggcacgca
    11221 ctttggccac ttcggctgtg acgccttcca gacgctgctc aatcaacact ttcagcgcct
    11281 gaccttcggt ttcgcgcgcg atgataaagt catccagggt gccatccaac gccgcgagaa
    11341 tttcagctgc aatcgcgtcg agatcttgct cctgggctgc catcacgccc ggccagcgta
    11401 gaatatcaac cgggttgatt tctccttcgt cgctctgcat ttttacccag ttcgcggcag
    11461 ttaccagctg tttagccagt ttttcgttga ggatcagctc accttgcgcg ctaacatctg
    11521 gctcatagcg cagagtacat tccactttac cgcgcgtcag gcgagaacga atacgctcgc
    11581 gaacgacagg ttcaaggcta cggaactgct ccggcagacg aaagtaagtt tccagataac
    11641 gctggtttac cgagcgcatt tcccaggttg cgctccccca ttcacccttg atttcacgcc
    11701 gggcgtaggc ggtcatactg cggatcatag acgttcctgt ttataaaagg agaggtggaa
    11761 ggattatagc catcgatgcc ttgtcaggat aggaataacc gccggaagtc cgtataatgc
    11821 gcagccacat ttgtttcaag ccggagattt caatatgcgt ccagcaggcc gtagcaataa
    11881 tcaggtgcgt cccgttaccc tgactcgtaa ctatacaaaa catgcagaag gctcggtgct
    11941 ggtcgaattt ggcgatacca aagtgttgtg taccgcctct attgaagaag gcgtgccgcg
    12001 cttcctgaaa ggtcagggcc agggctggat caccgcagag tacggtatgc tgccacgttc
    12061 tacccatacc cgtaacgcgc gtgaagcggc gaaaggtaag cagggtggac gcacaatgga
    12121 aatccagcgt ctgatcgccc gtgctcttcg cgcggcagta gatttgaaag cactgggtga
    12181 gttcaccatt acgctggact gcgacgtgct tcaggctgat ggtggcacgc gtaccgcgtc
    12241 gattacgggt gcctgcgtgg cgctggcaga tgcgctacag aagctggtgg aaaacggcaa
    12301 gctgaaaact aatccaatga aagggatggt agccgcggtc tctgtcggga ttgtgaacgg
    12361 cgaagcggtt tgcgatctgg aatacgttga agactctgcc gcagagaccg acatgaacgt
    12421 agtgatgacc gaagacgggc gtatcattga agtgcagggg acggcagaag gcgaaccgtt
    12481 cacccacgaa gagttactca ccttgttggc gctggcccga gggggaatcg aatccattgt
    12541 agcgacgcag aaggcggcgc tggcaaactg atttttaagg cgactgatga gtcgcctttt
    12601 ttttgtctgt agaaaagtaa gatgaggagc gaaggcatga aaccatatca gcgccagttt
    12661 attgaatttg cgcttagcaa gcaggtgtta aagtttggcg agttcacgct gaaatccggg
    12721 cgcaaaagcc cctatttctt caacgccggg ctgtttaata ccgggcgcga tctggcactg
    12781 ttaggccgtt tttacgctga agcattggtg gattccggca ttgagttcga tctgctgttt
    12841 ggccctgctt acaaagggat cccgattgcc accactaccg ccgtggcgct ggcggagcat
    12901 catgaccttg acctgccgta ctgctttaac cgcaaagagg caaaagacca cggtgaaggc
    12961 ggtaatctgg ttggtagcgc gttacaagga cgcgtaatgc tggtagatga tgtgatcacc
    13021 gccggaacgg cgattcgcga gtcgatggaa attattcagg ctaatggcgc gacgcttgca
    13081 ggcgtgttga tttcgcttga tcgtcaggaa cgcgggcgcg gcgaaatttc ggcgattcag
    13141 gaagttgagc gtgattacaa ctgcaaggtg atctctatca tcaccctgaa agacctgatt
    13201 gcttacctgg aagagaagcc agaaatggcg gaacatctgg cggcggttaa ggcctatcgc
    13261 gaagagtttg gcgtttaacg aaactcgccg gatgaaaagt catccggcgt catattactg
    13321 caactgtgcg gcaattagcg gccagcgggc gtcaaaatca tccgtcgggc ggtatttaaa
    13381 ctcgctgcgg acaaaacgtg acagcatacc ttcacagaag gccaggatct ggcttgccag
    13441 cagggtttca tcggtggtgt aaccttcacc ctcacgcatt ctcttttcac gcaatacctg
    13501 acgcaactgc gcttcaatac gctcgaacag ctggttgatg cgcccttgca gacgatcctg
    13561 ttcgaacatt agcgcatgac cagtgaggat gcgagtcagg ccaggattac gctcaccaaa
    13621 accgaggagc aacaacacga tcagacgcag gcgcgctgtg gtgtctttct catctttcag
    13681 aatcaggttg atacgagtaa tcaggctatc ttcgataaac tcaatcaggc tatcgaacat
    13741 gcgggtctta ctggggaagt ggcgatacag tgccgcttcg gaaacgccga cagaggcggc
    13801 cagttttgcc gtcgtgatac gttggcttcc atcgctggat tccagcatca gcgccagaga
    13861 ctgaagtatt tcctcgcgac ggttcctttt cgcagtttgt ttttctgcca tgttacaaaa
    13921 tacccctgaa aataagcact tgccaggcgg cacccacgct atgaccgcaa acgaaatgtt
    13981 tgcggctatg ttatgacgtt attcggatgc gtatgtgtta ctgacgacca gagtgaccaa
    14041 agccgccttc accgcggtcg gtggcgtcga aatcttccac cagattaaat tcagcctgta
    14101 ctaccggaac aaaaatcatc tgggcgatgc gttcgccagg ttgaatggtg aagctgtcct
    14161 gaccacggtt ccacacggaa atcatcaact ggccctgata gtcagaatcg atcaatccta
    14221 ccaggttacc aagcacgata ccgtgcttat gtcccaatcc ggagcgcggc agcatcattg
    14281 ccgccagtga aggatcggca atatgaatcg ccagcccggt cggaaccagc gtagtgtcac
    14341 ccggagccag ttctacggcg tcgtcgagac aggcacgcag gtcaagtccg gcagagccag
    14401 aggtggcata agtcgggagc ggaaattcct tcccaacgcg cgggtccaga atcttaacgt
    14461 cgattttttt catcataacg ggtcacgatc tcgtcgagta ataattggcc aaggagctct
    14521 ttgcgctcaa gcggtaagac tttatctccg tcctgccaga aaaggtgtaa tgcgttgttg
    14581 tcgctgttaa atccttgagt tggctgggaa acatcgttcg cgcagatcag atcaaggttt
    14641 ttacggatac gtttttgccg ggcgtattct tccacattat ttgtttcggc ggcaaatcca
    14701 acgacgtagg gtcgatggtc ttttagtgcg gcaacgcctg cgacgatatc ggggttttta
    14761 accattttta ttgttaattc atcaccctgc gtggcctgct ttttgatttt ttctggggcc
    14821 acggtagctg cgcgataatc cgccacggcg gcgcaaccga taaaaatatt ttgctgctgt
    14881 acagaagcat tcacggcggc ttccatttcc agcgcggtca tcacatcaac acggttaaca
    14941 aacggtggcg tcggtagtga agccggacct gataccagcg tgacgttcgc gccacgacgg
    15001 gctgcggcgg cggcgatagc aaaacccatc ttgccggagc tgtgattaga gatataacgc
    15061 acgggatcga gcggttcacg cgtcggaccg gcggtaatca taatgttcag atgtttcagg
    15121 tcgttgacgg gcgaaaaatg cgctaccgcc atatccacaa tggttaacgg atcgagcatt
    15181 cgtcctgggc cgatatcacc acaagcctga ctgccgctgt ctggccccca gatgagcaaa
    15241 ccacgcgagg caagcacctc taaattatgc tgcgtggcag cggcacggta catctgctgg
    15301 ttcatggcgg ggagcacggc tacaggcgca ggtgtagcca gacaaatcgt cgataccagg
    15361 tcattcgcca ttccggcagc aacacgggca attaaatctg ccgtggcagg ggcgagaatc
    15421 actaaatcag cccatttacc cagctcaata tggcccatag cggcttcggc tgccgggtcc
    15481 agcagactgt cggaaacggg ataaccagaa accgcctgca agctaagtgg ggtgataaag
    15541 gcttttgccg cttcggtcat ggctacgcgg acatcggccc cgcgatcgcg caaacgacgc
    15601 accagttcag gggttttata ggcagcaata ccgccgctaa cgccgagaac gattttttta
    15661 ccggccaggc tcatgatgat ttttcctgtt gggtgacacc agaagttggc gattttatca
    15721 caatactttt gttgtcgtgc cttcacctga gattcacttt gcgaggcgct ttccaggatt
    15781 gaaaactggc cgtcgattta aaggaacggc tatgacagga tgcgagcacc acaaaggagg
    15841 tgaaggtgaa aaacaatgta cagctgttga tgccgcgcga aaaaatgctg aagtttggta
    15901 ttagcgcctt aacggatgtc gagctgctgg cgctatttct gcgtaccgga acgcgcggta
    15961 aagatgtatt aaccctggca aaagagatgc tggagaattt cggctctctt tatggcttgt
    16021 taacctctga atatgagcaa tttagtggcg ttcatggaat tggcgtggcg aaattcgccc
    16081 agttaaaggg gattgccgaa ctggcgcggc gttactacaa cgtacggatg cgtgaagaaa
    16141 gccctttact cagcccggag atgacgaggg aatttttaca aagccagctc acgggcgagg
    16201 agcgggagat ctttatggtg atctttctcg actcccaaca cagggttata acgcatagcc
    16261 gtcttttttc cggcacgcta aaccatgttg aagtccatcc tcgggaaatt atccgcgaag
    16321 cgataaaaat aaacgcctcg gcgctgatcc ttgcgcataa tcacccttcg ggttgtgctg
    16381 aacccagtaa agcggataaa ctcattactg aacggataat aaagagttgt cagttcatgg
    16441 atttacgcgt gctcgaccat atcgtgattg ggcgtggaga gtatgtttct tttgccgaac
    16501 gcggctggat ttaacccgct atgcgcgatc cttcgggatc tttgtctgtt cgggacttga
    16561 gcacatcgct gagtcagcgt atactacgcc acctttgaga atctcgggtt tggcatttgg
    16621 gcctggcaat cgagagttca catagaactg cgatgaccgg gctgtaaagc ctgacgaggc
    16681 gccaataccc catacgaagc tcgagctaat ttgatttttg gagaatagac atgtcccgag
    16741 tctgccaagt tactggcaag cgtccggtga ccggtaacaa ccgttcccac gcactgaacg
    16801 cgactaaacg ccgtttcctg ccgaacctgc actctcaccg tttctgggtt gagagcgaga
    16861 agcgttttgt caccctgcgc gtatctgcta aaggtatgcg tgtaatcgat aaaaaaggca
    16921 tcgatacagt tctggctgaa ctgcgtgccc gtggcgaaaa gtactaagta cttagaggaa
    16981 ataaatcatg gctaaaggta ttcgtgagaa aatcaagctg gtttcttctg ctggtactgg
    17041 tcacttctat accactacga agaacaaacg tactaagccg gaaaaactgg aactgaaaaa
    17101 attcgatcca gttgttcgcc agcacgtgat ctacaaagaa gcgaaaatca aataatttcc
    17161 gctttgatgt aacaaaaaac ctcgccccgg cggggttttt tgttatctgc ttgcccccat
    17221 attgactgca tctgttcatt cctggagatg ctatgcctga attacccgaa gttgaaacca
    17281 gccgtcgcgg catagaaccg catctcgctg gtgcaaccat tcttcatgcg gtggtgcgca
    17341 acggacgctt gcgctggccg gtttcagaag agatctaccg tttaagcgac caaccagtgc
    17401 ttagcgtgca gcgccgggct aaatatctgc tgctggagct gcctgagggc tggattatca
    17461 ttcatttggg gatgtctggc agcctgcgca tccttccaga agaacttccc cctgaaaagc
    17521 atgaccatgt ggatttggtg atgagcaacg gcaaagtgct gcgctacacc gatccgcgcc
    17581 gctttggtgc ctggctatgg accaaagagc tggaagggca caacgtgctg gcccatcttg
    17641 gaccggagcc gcttagtgac gatttcaacg gtgagtacct gcatcagaag tgcgcgaaga
    17701 aaaaaacggc gattaaaccg tggctgatgg ataacaagct ggtggtaggc gtagggaata
    17761 tctatgccag cgaatcgctg tttgcggcgg ggatccatcc ggatcggctg gcgtcatcac
    17821 tgtcgctggc ggagtgtgaa ttgttagctc gggtgattaa agcggtgttg ctgcgttcga
    17881 ttgagcaggg cggtacaacg ctgaaagatt ttctgcaaag tgatggtaaa ccgggctatt
    17941 tcgctcagga attgcaggtt tacgggcgaa aaggtgagcc gtgtcgggtg tgcggtacgc
    18001 cgattgtggc gactaaacat gcgcagcggg caacgtttta ttgtcggcag tgccagaagt
    18061 aattgatgcg cgccggatgg cataccatcc ggcataaacg ctacgctaac ttcgccatca
    18121 gcgcctgatg gacattctcc ggcaggaaat gggtgacatc gccctgatgg cgcgccacct
    18181 ctttcaccaa cgatgaagag ataaacgacc actctttcga cggcatcaga aacacacttt
    18241 ccagctccgg cattaagtgg cgattcatat gcgccagctg catttcatat tcaaaatccg
    18301 ccaccgcacg caggccacga atcagcaccg tagcgtgttg attactggcg aagttcgcca
    18361 tcaaatcact aaacccgacc acttccacgt tccccagatg tgcggttgcc tgctgtgcca
    18421 gttccacacg ctcttcaagg gtaaacatcg gttttttact gggactagcg gcaatcgcca
    18481 gaataacgtg atcgaacatc tgcgtggcgc gcgtcacgat atcgatatga ccattggtaa
    18541 tgggatcgaa agtacccgga taaatcgccc gtttttgcat aacaacctca atgcgttttc
    18601 ggtggcaggt aaggttccag cagttgaagc agacgctgta gtgcgccctg gttttgatac
    18661 agcacttcaa cggcatgacg gccatagaaa ctacggtaat cggcgtcggt gagtaaagag
    18721 gaaacctctt ttgcaagcgt agtggcatcg gtaacggtaa tcagcccgct tgcctgctcc
    18781 agccgcgcgc aaatgtcttt aaagttaaac gtatgcggcc ccatcaatac cggaatagcg
    18841 tgtgcggccg cttccagcgg attatgccca ccacgttcaa ccagtgaacc gccaacaaag
    18901 gcgagatcgg caatgccgta cagtaacatc aactcgccca tcgtatcgcc aaccacaacc
    18961 tgcgtgctgg tggaggggac ttcccctgaa gatcgtgtga tatagcttag tccagcctgg
    19021 cggacaaggt taatcgcatc cgggaagcgt tctggatgac ggggtaccag gatgagcaat
    19081 aaattcggga attgctgtaa caatgcctga tgtgcggcga tcaccacact ctcttcgcct
    19141 tcatgagtgc tggtggcaat ccataccggg cggtgtggtg cccattggcg gcgcagcgtc
    19201 acggctttag cagccaactg cggcgttaca gaaatatcga atttcaggct accggtaacg
    19261 gtcacctgat tattttttgc gcccaacgcc acaaaacgag caccatcttc ttcattttgg
    19321 gcggcaatca gcgtaatacg acgcagcaag cgacggacga atttacccag tttggcataa
    19381 cctgcggccg agcgggcaga gagtcgcgcg ttagcgatca ccagcggaat tttacgttta
    19441 tgtagcgccg caatcaggtt aggccatagt tcggtttcca taatcaacac cagtttaggg
    19501 tcgactttat tcaggaaacg gttgagtgca tcgggcaggt catacggcag ataaacgtgc
    19561 tgaacatcct tcccgaaagc cgattgtacg cgctccgaac cggttggagt catggttgtt
    19621 acggtaatcg gtaaatcagg ataacgatga cgcagtgcgc gcaccaacgg gatcgccgcc
    19681 agagtttcac cgacggagac ggagtgcagc ataatgccgc ctggttttag cggatggcgg
    19741 taaaaaccgt aacgttcacc ccagcgtttt cgataggccg gagccttacg tccgcgcacc
    19801 cagagccgta tccagatcag cggctgaata aggtagagaa gggcggtgta aagcaattcg
    19861 agcatagtaa atagctgact tatggatgtg ctggggattc tatgtattta gctgtggctt
    19921 taccattact tttcccgttt ttgacttaaa tagcaattta cccgccaatt cttgctgaac
    19981 atctatactt tttttgtatt tttatgcgat tcattgattt ctgcagtacg tgcaggaaga
    20041 aatcgcagag caacagtaaa agaatatggt tgctaatgat tttatttgat tgatttcttt
    20101 attgaaaatc aacaatgtgc ttaaaaatca ttcgagtcgg attgccgcga aagaagtgat
    20161 acactagcgc gtcaatatca gtgcggtatc agtattcagg tagctgttga gcctggggcg
    20221 gtagcgtgct ttttcagctt caccttaatc ggataatctc aacaaaaaga gttacttgtg
    20281 gataagccat ttcgaagaat tttgctcatt aagatgcgtt ttcatgggga tatgttatta
    20341 actactcccg tcattagttc gctgaaaaaa aattaccctg acgcaaaaat cgatgtgctg
    20401 ctttatcagg acaccatccc gatcctgtct gaaaatccag agattaacgc gctctacggc
    20461 ataaaaaata aaaaagcaaa agcctcagaa aaaattacca acttttttca tctcatcaag
    20521 gtattacgtg ccaataagta tgaccttatc gttaatctta ccgatcaatg gatggttgct
    20581 atactggttc gcttattaaa tgcccgtgtg aaaatttccc aggattatca tcatcggcag
    20641 tctgcttttt ggcgtaatag tttcactcat ttggtgccgt tgcagggtgg aaatgtggtg
    20701 gaaagtaact tatccgtgct gacgccattg ggacttgaat cgttggtgaa gcagacaacc
    20761 atgagttacc cgcctgcaag ctggaaacgt atgcgtcgcg aacttgatca cgctggtgtt
    20821 ggacaaaatt atgtggttat ccaacctacg gcgcggcaaa tcttcaaatg ctgggacaac
    20881 gccaagtttt ccgctgtgat tgatgcctta catgctcgtg gttatgaagt cgttctgacg
    20941 tccggcccag ataaagacga tctggcctgc gtcaatgaaa ttgcgcaggg atgccagacg
    21001 ccaccagtaa cggcgctggc cggaaaggtg accttcccgg aacttggtgc gttaatcgat
    21061 catgcgcagc tgtttattgg cgttgattcc gcaccggcgc atattgccgc tgcagttaat
    21121 acgccgctga tatcgctgtt tggtgcgacg gaccatattt tctggcgtcc ctggtcaaat
    21181 aacatgattc aattctgggc gggagattac cgggaaatgc caacgcgcga tcagcgtgac
    21241 cgaaatgaga tgtatctttc ggttattccg gcggcagatg tcattgcagc tgtcgataaa
    21301 ttacttccct cctccacgac aggtacgtcg ttatgatcgt tgctttttgt ttatataaat
    21361 attttccctt tggcggtttg cagcgcgatt ttatgcgtat tgctcagaca gttgccgccc
    21421 gaggtcatca tgttcgggtt tatacccagt cgtgggaagg cgaatgccct gatgtatttg
    21481 aactgatcaa agtgccggtt aaatcgcata ccaatcacgg gcgcaatgcg gaatattttg
    21541 cctgggtgca aaaacattta cgtgaacacc ccgtcgatag agtcgttgga tttaataaaa
    21601 tgccggggct ggacgtttat tatgccgctg atgtttgtta tgccgagaaa gtagcgcagg
    21661 agaaaggctt tttctatcgc ctgacgtcac gttatcgcca ttatgccgcc tttgagcggg
    21721 caaccttcga acagggcaag ccgacacagc tgctgatgct gacagataag caaatcgccg
    21781 atttccagaa acattatcag actgaagcgg agcgttttca tattctgcct ccggggattt
    21841 atcctgatcg taaatatagc cagcagccag ccaatagccg tgaaatcttc cgtaagaaga
    21901 atggaataac cgaacaacaa tatttattgt tgcaggtcgg ttcagacttc acgcgtaaag
    21961 gtgtcgatcg ttccattgaa gcacttgctt cgttaccgga ttcgctgcgc cacaacacat
    22021 tactatatgt tgttgggcag gataaaccgc gaaaatttga ggcgctggca gaaaaacgcg
    22081 gcgtgcgcag taatgttcac ttcttctcgg ggcgcaacga tgtctcggaa ttaatggcgg
    22141 cggcggattt attacttcat cctgcctacc aggaagcggc gggaattgtg ctgctggaag
    22201 cgataactgc aggattaccg gtactaacaa cagccgtttg tggctatgcg cattatattg
    22261 tcgatgccaa ttgcggcgag gctattgctg agccattccg ccaggaaaca ttgaatgaga
    22321 ttttacgcaa agcgttaacg caatcttcat tgcgccaggc ttgggcggaa aatgcgcgac
    22381 attatgctga tacacaagat ttatacagtc tgccagagaa agcggcggac atcataacgg
    22441 gtggtctgga tggttgaact taaagagccg tttgccacgt tatggcgcgg taaagatcct
    22501 tttgaggaag ttaaaacctt gcagggtgag gtatttcgtg aactggaaac tcgccgcact
    22561 ctgcgctttg aaatggcggg caaaagctat tttctcaaat ggcatcgcgg cacgaccctg
    22621 aaagagataa tcaaaaattt actctcattg cggatgccag tattaggcgc agaccgcgaa
    22681 tggaatgcga ttcatcgact gcgggatgtc ggcgttgata ctatgtatgg ggtggcattc
    22741 ggcgaaaaag gcattaatcc gctgaccaga acctcgttta ttattaccga agatctgaca
    22801 ccaaccatca gtctggaaga ttactgtgct gactgggcga ccaactcacc agatgttcgc
    22861 gtaaagcgta tgcttattaa gcgtgtcgcg acgatggtgc gcgatatgca tgctgcgggc
    22921 attaaccacc gtgactgtta tatctgtcat ttcctgctgc acttgccttt ttccggtaag
    22981 gaagaggtgt taaaaatttc agtaattgac ctgcaccggg cgcagatacg tgcaaaagta
    23041 ccgcgtcgtt ggcgggataa agatcttatt ggactttatt tttcttcgat gaatattggc
    23101 ctgactcagc gggatatctg gcggtttatg aaagtgtatt ttgtcgcccc gcttaaagac
    23161 attatcaagc aggaacaagg gctgctgtcg caagcagaag aaaaagccac aaaaatcagg
    23221 gaaagaacga ttcgaaaatc gttgtaaccc gtaacttatt tttgccaaaa ttttggatac
    23281 agaataaata tgt
//
LOCUS       DADSYM010000070        23221 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2415
            SAMN08039579-rid11723173.denovo.070, whole genome shotgun sequence.
ACCESSION   DADSYM010000070 DADSYM010000000
VERSION     DADSYM010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039579
            Sequence Read Archive: SRR6362533
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 23221)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23221)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980162.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 03:39:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,484
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 4,347
            CDSs (with protein)               :: 4,347
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 43 of 85
            Pseudo Genes (incomplete)         :: 37 of 85
            Pseudo Genes (internal stop)      :: 19 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23221
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039579-rid11723173.denovo.070"
                     /strain="LGE2415"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8302 N 95.9628 W"
                     /collection_date="2015-08-24"
     gene            <1..321
                     /locus_tag="J1Y83_002369"
     CDS             <1..321
                     /locus_tag="J1Y83_002369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBA7630514.1"
                     /translation="TLEQLSKKFRASVSYICHEFTKEYRISPINYVIQRRMTEAKWSL
                     TNTELSQAEISWRVGYENVDHFAKLFLRHVGCSPSDYRRQFKNCFAEQEILSEFPQPV
                     SLAG"
     gene            complement(364..1515)
                     /locus_tag="J1Y83_002370"
     CDS             complement(364..1515)
                     /locus_tag="J1Y83_002370"
                     /EC_number="1.3.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HBA7630515.1"
                     /translation="MDFSLTEEQELLLASIRELITTNFPEEYFRTCDQNGTYPREFMR
                     ALADNGISMLGVPEEFGGIPADYVTQMLALMEVSKCGAPAFLITNGQCIHSMRRFGSA
                     EQLRKTAESTLETGDPAYALALTEPGAGSDNNSATTTYTRKNGKVYINGQKTFITGAK
                     EYPYMLVLARDPQPKDPKKAFTLWWVDSSKPGIKINPLHKIGWHMLSTCEVYLDNVEV
                     EESDMVGEEGMGFLNVMYNFEMERLINAARSTGFAECAFEDAARYANQRIAFGKPIGH
                     NQMIQEKLALMAIKIDNMRNMVLKVAWQADQHQSLRTSAALAKLYCARTAMEVIDDAI
                     QIMGGLGYTDEARVSRFWRDVRCERIGGGTDEIMIYVAGRQILKDYQNK"
     gene            complement(1529..3124)
                     /locus_tag="J1Y83_002371"
     CDS             complement(1529..3124)
                     /locus_tag="J1Y83_002371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate CoA-transferase YdiF"
                     /protein_id="HBA7630516.1"
                     /translation="MKPVKPPRINGRVPVLSAQEAVNYIPDEATLCVLGAGGGILEAT
                     TLITALADKYKQTQTPRNLSIISPTGLGDRADRGISPLAQEGLVKWALCGHWGQSPRI
                     SELAEQNKIIAYNYPQGVLTQTLRAAAAHQPGIISDIGIGTFVDPRQQGGKLNEVTKE
                     DLIKLVEFDNKEYLYYKAIAPDIAFIRATTCDSEGYATFEDEVMYLDALVIAQAVHNN
                     GGIVMMQVQKMVKKATLHPKSVRIPGYLVDIVVVDPDQTQLYGGAPVNRFISGDFTLD
                     DSTKLSLPLNQRKLVARRALFEMRKGAVGNVGVGIADGIGLVAREEGCADDFILTVET
                     GPIGGITSQGIAFGANVNTRAILDMTSQFDFYHGGGLDVCYLSFAEVDQHGNVGVHKF
                     NGKIMGTGGFIDISATSKKIVFCGTLTAGSLKTEITDGKLNIVQEGRVKKFIRELPEI
                     TFSGKIALERGLDVRYITERAVFTLKEDGLHLIEIAPGVDLQKDILDKMDFTPVISPE
                     LKLMDERLFIDAAMGFVLPEAAH"
     gene            complement(3270..4028)
                     /gene="aroD"
                     /locus_tag="J1Y83_002372"
     CDS             complement(3270..4028)
                     /gene="aroD"
                     /locus_tag="J1Y83_002372"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HBA7630517.1"
                     /translation="MKTVTVKDLVIGTGAPKIIVSLMAKDIASVKSEALAYREADFDI
                     LEWRVDHYADLSNVESVIAAAKILRETMPEKPLLFTFRSAKEGGEQAISTEAYIALNR
                     AAIDSGLVDMIDLELFTGDDQVKETVAYAHAHDVKVVMSNHDFHKTPEAEEIIARLRK
                     MQSFDADIPKIALMPQSTSDVLTLLAATLEMQEQYADRPIITMSMAKTGVISRLAGEV
                     FGSAATFGAVKKASAPGQISVNDLRTVLTILHQA"
     gene            complement(4059..4925)
                     /gene="ydiB"
                     /locus_tag="J1Y83_002373"
     CDS             complement(4059..4925)
                     /gene="ydiB"
                     /locus_tag="J1Y83_002373"
                     /EC_number="1.1.1.282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinate/shikimate dehydrogenase"
                     /protein_id="HBA7630518.1"
                     /translation="MDVTAKYELIGLMAYPIRHSLSPEMQNKALEKAGLPFTYMAFEV
                     DNDSFPGAIEGLKALKMRGTGVSMPNKQLACEYVDELTPAAKLVGAINTIVNDDGYLR
                     GYNTDGTGHIRAIKESGFDIKGKTMVLLGAGGASTAIGAQGAIEGLKEIKLFNRRDEF
                     FDKALAFAQRVNENTDCVVTVTDLADQQAFAEALASADILTNGTKVGMKPLENESLVN
                     DISLLHPGLLVTECVYNPHMTKLLQQAQQAGCKTIDGYGMLLWQGAEQFTLWTGKDFP
                     LEYVKQVMGFGA"
     gene            complement(4937..6202)
                     /locus_tag="J1Y83_002374"
     CDS             complement(4937..6202)
                     /locus_tag="J1Y83_002374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBA7630519.1"
                     /translation="MSQNKAFSTPFILAVLCIYFSYFLHGISVITLAQNMTSLAEKFS
                     TDNAGIAYLISGIGLGRLISILFFGVISDKFGRRAVILMAVIMYLLFFFGIPACPNLT
                     LAYCLAVCVGIANSALDTGGYPALMECFPKASGSAVILVKAMVSFGQMFYPMLVSYML
                     LNNIWYGYGLIIPGILFVLITLMLLKSKFPSQLVDASVANELPQMNSKPLVWLEGVSS
                     VLFGVAAFSTFYVIVVWMPKYAMAFAGMSEAEALKTISYYSMGSLVCVFIFAALLKKM
                     VRPIWANVFNSALATITAAIIYLYPSPLVCNAGAFVIGFSAAGGILQLGVSVMSEFFP
                     KSKAKVTSIYMMMGGLANFVIPLITGYLSNIGLQYIIVLDFTFALLALITAIIVFIRY
                     YRVFIIPENDVRFGEHKFSTRLNTIKHRG"
     gene            complement(6429..7643)
                     /locus_tag="J1Y83_002375"
     CDS             complement(6429..7643)
                     /locus_tag="J1Y83_002375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBA7630520.1"
                     /translation="MKNPYYPTALGLYFNYLVHGMGVILMSLNMASLETLWQTNAAGV
                     SIVISSLGIGRLSVLLFAGLLSDRFGRRPFIMLGMCCYMAFFFGILHTNNIIIAYVFG
                     FLAGMANSFLDAGTYPSLMEAFPRSPGTANILIKAFVSSGQFLLPLIISLLVWAELWF
                     GWSFMIAAGIMFINALFLYRCTFPPHPGRHLPVIKKTTSSTEHRCSIIDLASYSLYGY
                     ISMATFYLVSQWLAQYGQFVAGMSYTMSIKLLSIYTVGSLLCVFITAPLIRNTVRPTT
                     LLMLYTFISFIALLTVCLHPTFYVVIIFAFVIGFTSAGGVVQIGLTLMAERFPYAKGK
                     ATGIYYSAGSIATFTIPLITAHLSQRSIADIMWFDTAIAAIGFILALFIGLRSRKETR
                     HHTLKENIAPGG"
     gene            complement(7743..8099)
                     /locus_tag="J1Y83_002376"
     CDS             complement(7743..8099)
                     /locus_tag="J1Y83_002376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707588.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiL family protein"
                     /protein_id="HBA7630521.1"
                     /translation="MNAYELQALRHIFAMTIDECATWIAQTGDSESWRQWENGKCAIP
                     DCVVEQLLAMRQQRKKHLHAIIEKINNRIGNNTMRFFPDLTAFQQVYPDGNFIDWKIY
                     QSVAAELYAHDLERLC"
     gene            complement(8235..8342)
                     /gene="rprA"
                     /locus_tag="J1Y83_002377"
     ncRNA           complement(8235..8342)
                     /ncRNA_class="antisense_RNA"
                     /gene="rprA"
                     /locus_tag="J1Y83_002377"
                     /product="antisense sRNA RprA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00034"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00034"
     gene            complement(8528..9640)
                     /gene="ydiK"
                     /locus_tag="J1Y83_002378"
     CDS             complement(8528..9640)
                     /gene="ydiK"
                     /locus_tag="J1Y83_002378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter YdiK"
                     /protein_id="HBA7630522.1"
                     /translation="MVNVRQPRDVAQILLSVLFLAIMIVACLWIVQPFILGFAWAGTV
                     VIATWPVLLRLQKIMFGRRSLAVLVMTLLLVMVFIIPIALLVNSIVDGSGPLIKAISS
                     GDMTLPDLAWLNTIPVIGAKLYAGWHNLLDMGGTAIMAKVRPYIGTTTTWFVGQAAHI
                     GRFMVHCALMLLFSALLYWRGEQVAQGIRHFATRLAGVRGDAAVLLAAQAIRAVALGV
                     VVTALVQAVLGGIGLAVSGVPYATLLTVLMILSCLVQLGPLPVLIPAIIWLYWTGDTT
                     WGTVLLVWSGVVGTLDNVIRPMLIRMGADLPLILILSGVIGGLIAFGMIGLFIGPVLL
                     AVSWRLFAAWVEEVPPPTDQPEEILEELGEIEKPNK"
     gene            9854..12910
                     /locus_tag="J1Y83_002379"
     CDS             9854..12910
                     /locus_tag="J1Y83_002379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HBA7630523.1"
                     /translation="MIPQISQAPGVVQLVLNFLQELEQQGFTGDTATSYADRLTMSTD
                     NSIYQLLPDAVVFPRSTADVALIARLAAQERYSSLIFTPRGGGTGTNGQALNQGIIVD
                     MSRHMNRIIEINPEEGWVRVEAGVIKDQLNQYLKPFGYFFAPELSTSNRATLGGMINT
                     DASGQGSLVYGKTSDHVLGVRAVLLGGDILDTQPLPVELAETLGKSNTTIGRIYNTVY
                     QRCRQQRQLIIDNFPKLNRFLTGYDLRHVFNDEMTEFDLTRILTGSEGTLAFITEARL
                     DITRLPKVRRLVNVKYDSFDSALRNAPFMVEARALSVETVDSKVLNLAREDIVWHSVS
                     ELITDVPDKEMLGLNIVEFAGDDEALIDERVNALCVRLDELIASQQAGVIGWQVCREL
                     AGVERIYAMRKKAVGLLGNAKGAAKPIPFAEDTCVPPEHLADYIAEFRALLDSHGLSY
                     GMFGHVDAGVLHVRPALDMCDPQQEILMKQISDDVVALTAKYGGLLWGEHGKGFRAEY
                     SPAFFGEELFAELRKVKAAFDPHNRLNPGKICPPEGLDAPMMKVDAVKRGTFDRQIPI
                     AVRQQWRGAMECNGNGLCFNFDARSPMCPSMKITQNRIHSPKGRATLVREWLRLLADR
                     GVDPLKLEQELPESGVSLRTLIARTRNSWHANKGEYDFSHEVKEAMSGCLACKACSTQ
                     CPIKIDVPEFRSRFLQLYHTRYLRPLRDHLVATVESYAPLMARAPKTFNFFINQPLVR
                     KLSEKHIGMVDLPLLSVPSLQQQMVGHRSANMTLEQLEALNAEQKARTVLVVQDPFTS
                     YYDAQVVADFVRLVEKLGFQPVLLPFSPNGKAQHIKGFLNRFAKTAKKTADFLNRMAK
                     LGMPMVGVDPALVLCYRDEYKLALGEERGEFNVLLANEWLASALESQPVATVSGESWY
                     FFGHCTEVTALPGAPAQWAAIFARFGAKLENVSVGCCGMAGTYGHEAKNHKNSLGIYE
                     LSWHQAMQRLPRNRCLATGYSCRSQVKRVEGTGVRHPVQALLEIIK"
     gene            12907..13317
                     /gene="menI"
                     /locus_tag="J1Y83_002380"
     CDS             12907..13317
                     /gene="menI"
                     /locus_tag="J1Y83_002380"
                     /EC_number="3.1.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA hydrolase"
                     /protein_id="HBA7630524.1"
                     /translation="MIWKRKITLEALNAMGEGNMVGLLDIRFEHIGDDTLEATMPVDS
                     RTKQPFGLLHGGASVVLAESIGSVAGYLCTEGEQKVVGLEINANHVRSTREGRVRGVC
                     KPLHLGSRHQVWQIEIFDEKGRLCCSSRLTTAIL"
     gene            13417..13605
                     /locus_tag="J1Y83_002381"
     CDS             13417..13605
                     /locus_tag="J1Y83_002381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA7630525.1"
                     /translation="MSTQLDPTQLAIEFLRRDQSNLSPAQYLKRLKQLELEFADLLTL
                     SSAELKEEIYFAWRLGVH"
     gene            14154..14522
                     /gene="sufA"
                     /locus_tag="J1Y83_002382"
     CDS             14154..14522
                     /gene="sufA"
                     /locus_tag="J1Y83_002382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold SufA"
                     /protein_id="HBA7630526.1"
                     /translation="MDMHSGTFNPQDFAWQGLTLTPAAAVHIRELVAKQPGMIGVRLG
                     VKQTGCAGFGYVLDSVSEPDKDDLLFEHDGAKLFVPLQAMPFIDGTEVDFVREGLNQI
                     FKFHNPKAQNECGCGESFGV"
     gene            14531..16018
                     /gene="sufB"
                     /locus_tag="J1Y83_002383"
     CDS             14531..16018
                     /gene="sufB"
                     /locus_tag="J1Y83_002383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416198.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HBA7630527.1"
                     /translation="MSRNTEATDDVKTWTGGPLNYKEGFFTQLATDELAKGINEEVVR
                     AISAKRNEPEWMLEFRLNAYHAWLEMEEPHWLKAHYDKLNYQDYSYYSAPSCGNCDDN
                     CASEPGAVQQTGANAFLSKEVEAAFEQLGVPVREGKEVAVDAIFDSVSVATTYREKLA
                     EQGIIFCSFGEAIHDHPELVRKYLGTVVPGNDNFFAALNAAVASDGTFIYVPKGVRCP
                     MELSTYFRINAEKTGQFERTILVADEDSYVSYIEGCSAPVRDSYQLHAAVVEVIIHKN
                     AEVKYSTVQNWFPGDNNTGGILNFVTKRALCEGENSKMSWTQSETGSAITWKYPSCIL
                     RGDNSIGEFYSVALTSGHQQADTGTKMIHIGKNTKSTIISKGISAGHSQNSYRGLVKI
                     MPTATNARNFTQCDSMLIGANCGAHTFPYVECRNNSAQLEHEATTSRIGEDQLFYCLQ
                     RGISEEDAISMIVNGFCKDVFSELPLEFAVEAQKLLAISLEHSVG"
     gene            16028..16774
                     /gene="sufC"
                     /locus_tag="J1Y83_002384"
     CDS             16028..16774
                     /gene="sufC"
                     /locus_tag="J1Y83_002384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008500629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HBA7630528.1"
                     /translation="MLSIKDLHVSVEDKAILRGLSLDVRPGEVHAIMGPNGSGKSTLS
                     ATLAGREDYEVTSGTVEFKGKDLLALSPEDRAGEGIFMAFQYPVEIPGVSNQFFLQTA
                     LNAVRSYRGQETLDRFDFQDLMEEKIALLKMPEDLLTRSVNVGFSGGEKKRNDILQMA
                     VLEPELCILDESDSGLDIDALKVVADGVNSLRDGKRSFIIVTHYQRILDYIKPDYVHV
                     LYQGRIVKSGDFTLVKQLEEQGYGWLTEQQ"
     gene            16749..18020
                     /gene="sufD"
                     /locus_tag="J1Y83_002385"
     CDS             16749..18020
                     /gene="sufD"
                     /locus_tag="J1Y83_002385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HBA7630529.1"
                     /translation="MAGLPNSSNALQQWHHLFEAEGAKRSPQAQQHLQQLLRTGLPTR
                     KHENWKYTPLDGLTNSQFVSIAGEISPQQRDALALTLDAVRLVFVDGRYVPALSDATE
                     GSGYEVSINDDRQGLPDAIQAEVFLHLTESLAQSVTHIAVKRGQRPAKPLLLMHITQG
                     VAGEEVNTAHYRHHLDLAEGAEATVIEHFVSLNDARHFTGARFTINVAANAHLQHIKL
                     AFENPLSYHFAHNDLLLADDATAFSHSFLLGGAVLRHNTSTQLNGENSTLRINSLAMP
                     VKNEVCDTRTWLEHNKGFCNSRQLHKTIVSDKGRAVFNGLINVAQHAIKTDGQMTNNN
                     LLMGKLAEVDTKPQLEIYADDVKCSHGATVGRIDDEQMFYLRSRGINQQDAQQMIIYA
                     FAAELTEALRDEGLKQQVLARIGQRLPGGAR"
     gene            18017..19237
                     /gene="sufS"
                     /locus_tag="J1Y83_002386"
     CDS             18017..19237
                     /gene="sufS"
                     /locus_tag="J1Y83_002386"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase SufS"
                     /protein_id="HBA7630530.1"
                     /translation="MTFSVDKVRADFPVLSREVNGLPLAYLDSAASAQKPSQVIDAEA
                     EFYRHGYAAVHRGIHTLSAQATEKMENVRKRASLFINARSAEELVFVRGTTEGINLVA
                     NSWGNSNVRAGDNIIISQMEHHANIVPWQMLCARVGAELRVIPLNPDGTLQLETLPTL
                     FDEKTRLLAITHVSNVLGTENPLAEMITLAHQHGAKVLVDGAQAVMHHPVDVQALDCD
                     FYVFSGHKLYGPTGIGILYVKEALLQEMPPWEGGGSMIATVSLSEGTTWTKAPWRFEA
                     GTPNTGGIIGLGAALEYVSALGLNNIAEYEQNLMHYALSQLAAVPDLTLYGPQNRLGV
                     IAFNLGKHHAYDVGSFLDNYGIAVRTGHHCAMPLMAYYNVPAMCRASLAMYNTHEEVD
                     RLVTGLQRIHRLLG"
     gene            19250..19666
                     /gene="sufE"
                     /locus_tag="J1Y83_002387"
     CDS             19250..19666
                     /gene="sufE"
                     /locus_tag="J1Y83_002387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfuration protein SufE"
                     /protein_id="HBA7630531.1"
                     /translation="MALLPDKEKLLRNFLRCANWEEKYLYIIELGQRLPELRDEDKSP
                     QNSIQGCQSQVWIVMRQNAQGIIELQGDSDAAIVKGLIAVVFILYDQMTPQDIVNFDV
                     RPWFEKMALTQHLTPSRSQGLEAMIRAIRAKAAALS"
     gene            19815..20819
                     /gene="ldtE"
                     /locus_tag="J1Y83_002388"
     CDS             19815..20819
                     /gene="ldtE"
                     /locus_tag="J1Y83_002388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase LdtE"
                     /protein_id="HBA7630532.1"
                     /translation="MKRASLLTLTLIGAFSAIQAAWAVDYPLPPTGSRLVGQNQTYTV
                     QEGDKNLQAIARRFDTAAMLILEANNTIAPVPKPGTTITIPSQLLLPDAPRQGIIVNL
                     AELRLYNYPPGENIVQVYPIGIGLQGLETPVMETRVGQKIPNPTWTPTAGIRQRSLER
                     GIKLPPVVPAGPNNPLGRYALRLAHGNGEYLIHGTSAPDSVGLRVSSGCIRMNAPDIK
                     ALFSSVRTGTPVKVINEPVKYSVEPNGMRYVEVHRPLSAEEQQNVQTMPYTLPAGFTQ
                     FKDNKAVDQKLVDKALYRRAGYPVAVSSGATPAASNAPSVESAQNGEPEQGNMLRATQ
                     "
     gene            complement(20882..21118)
                     /locus_tag="J1Y83_002389"
     CDS             complement(20882..21118)
                     /locus_tag="J1Y83_002389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006675846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major outer membrane lipoprotein"
                     /protein_id="HBA7630533.1"
                     /translation="MKATKLVLGAVILGSTLLAGCSSNAKIDQLSSDVQTLNAKVDQL
                     SNDVNAMRSDVQAAKDDAARANQRLDNMATKYRK"
     gene            complement(21429..22841)
                     /locus_tag="J1Y83_002390"
     CDS             complement(21429..22841)
                     /locus_tag="J1Y83_002390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010346010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase I"
                     /protein_id="HBA7630534.1"
                     /translation="MKKTKIVCTIGPKTESEEMLAKMLDAGMNVMRLNFSHGDYAEHG
                     QRIQNLRNVMSKTGKTAAILLDTKGPEIRTMKLEGGNDVSLKAGQTFTFTTDKSVIGN
                     SEMVAVTYEGFTTDLSVGNTVLVDDGLIGMEVTAIEGNKVICKVLNNGDLGENKGVNL
                     PGVSIALPALAEKDKQDLIFGCEQGVDFVAASFIRKRSDVIEIREHLKAHGGENIHII
                     SKIENQEGLNNFDEILEASDGIMVARGDLGVEIPVEEVIFAQKMMIEKCIRARKVVIT
                     ATQMLDSMIKNPRPTRAEAGDVANAILDGTDAVMLSGESAKGKYPLEAVSIMATICER
                     TDRVMNSRLEFNNDNRKLRITEAVCRGAVETAEKLDAPLIVVATQGGKSARAVRKYFP
                     DATILALTTNEKTAHQLVLSKGVVPQLVKEITSTDDFYRLGKELALQSGLAHKGDVVV
                     MVSGALVPSGTTNTASVHVL"
BASE COUNT         6028 a         5610 c         5849 g         5734 t
ORIGIN      
        1 actttagagc agttatcgaa aaaatttcgt gccagcgtca gttatatctg ccatgaattt
       61 accaaagagt atcgtatttc ccctattaac tatgttattc aacggcgtat gacggaagcg
      121 aaatggtcac tcactaatac tgaattatca caggcagaga tttcctggcg tgtgggttat
      181 gaaaatgtcg atcactttgc caaactgttt ttgcgccatg tcggctgttc gcccagcgat
      241 taccgcaggc aatttaaaaa ctgttttgcg gaacaagaaa tcctatctga atttcctcaa
      301 ccagtaagtc ttgccggata aatctggcgg cgcagtttgt taagaagctg cgccgcctgc
      361 agattatttg ttctgataat ctttcaggat ctgccgacct gctacgtaaa tcataatttc
      421 gtctgtaccg ccgccgatac gttcacaacg gacatcacgc cagaagcggg agacgcgcgc
      481 ctcatcggta tagcccagac cgcccatgat ttgaatcgca tcatcaatga cttccattgc
      541 ggtacgtgcg cagtacagtt ttgccagcgc cgcgctggtg cgcagtgact gatgctgatc
      601 ggcttgccat gccactttca acaccatgtt gcgcatgttg tcgatcttaa tcgccatcag
      661 cgccagtttt tcctggatca tctggttatg accaatgggc ttaccaaaag cgatacgctg
      721 gttggcatag cgggcggcat cttcgaatgc gcattcggca aagccggtgc tgcgcgcggc
      781 gttgatcagg cgctccatct caaagttgta catcacattg aggaaaccca ttccttcttc
      841 gcccaccatg tcgctctctt caacttccac gttgtcgaga tagacttcgc aggtgctgag
      901 catatgccag ccgattttat gcagtggatt gattttaatg ccgggtttac tggagtcgac
      961 ccaccacaga gtaaaggcct ttttcggatc tttcggttgc ggatcgcgcg ccaacaccag
     1021 catatatggg tactctttcg caccggtaat aaaggttttc tgtccgttga tgtaaacctt
     1081 gccgttttta cgcgtgtaag tggtagtggc actgttgttg tctgagcctg cgcctggctc
     1141 tgtcaatgcc agggcatagg cgggatcacc cgtttccagg gtgctctctg ccgttttacg
     1201 tagctgctct gcagaaccga aacggcgcat actgtgaata cattgaccat tggtaatcaa
     1261 aaatgccgga gcaccgcatt ttgacacttc catcagcgcc agcatttggg tgacgtaatc
     1321 cgcagggata ccaccaaatt cttccggcac gccaagcatg gaaataccgt tatccgccag
     1381 tgcccgcata aactcacgcg ggtatgtccc gttttgatcg caggtgcgga aatactcttc
     1441 cggaaagtta gtcgtaatca gttcgcgaat actggccagc agcagttctt gttcttcagt
     1501 taaagagaaa tccatcatta tactcctgtt aatgagccgc ttcaggcagg acaaaaccca
     1561 tcgccgcatc gataaataat ctttcgtcca tcagtttgag ttctggcgaa atcactgggg
     1621 tgaaatccat tttgtcgaga atatcttttt gtaaatcgac gccaggagcg atttcgatta
     1681 aatgcaggcc gtcttctttt agcgtgaata cggcgcgctc agtgatataa cgaacatcca
     1741 gccctcgctc gagagcgatt tttccgctga aagttatttc cggtagttcc cgaataaatt
     1801 tcttcacccg tccttcctgg acgatattta atttgccgtc ggtaatttct gtttttaaac
     1861 tgcccgcagt taatgtgccg cagaaaacga ttttcttcga agtggcactg atatcaataa
     1921 atccaccggt gcccatgatt ttaccattga atttatgcac gccgacgtta ccgtgctggt
     1981 cgacttcagc aaaactcaaa taacaaacat ccagaccacc accgtgataa aaatcaaact
     2041 gggacgtcat atccagaatg gcgcgggtat tcacgttcgc gccaaaggcg atcccctgtg
     2101 aagtaatacc gccaatcgga cctgtttcta ccgtcagaat aaagtcatca gcacaacctt
     2161 cttcccgggc gaccaggcca atgccgtcag caataccgac gccgacattc cccactgcgc
     2221 ctttgcgcat ttcgaataat gcacgccgtg caactaattt acgttggttt aggggcaggc
     2281 taagtttggt gctgtcatca agagtgaagt caccagaaat aaagcggtta accggcgcac
     2341 cgccatacag ttgggtttga tccggatcga ccaccacaat atccaccaga taacccggaa
     2401 tacggacaga tttaggatgc agcgtggctt tcttaaccat tttctgcacc tgcatcatca
     2461 caatacctcc gttattgtgc accgcctggg caataaccaa tgcgtcgaga tacatcacct
     2521 catcttcaaa agtggcgtag ccttcgctgt cgcaggtggt agcgcgaatg aaggcgatat
     2581 ctggcgctat cgctttgtaa tagagatatt ctttgttatc aaactcgacc agtttaatca
     2641 ggtcttcttt agtgacttca ttcagtttgc cgccttgctg gcgtggatcg acaaatgttc
     2701 cgatgccaat atcactaata ataccaggct ggtgggctgc ggcggcgcgt aaggtttgtg
     2761 taagtacacc ttgtggatag ttataagcaa taattttatt ttgttctgcg agttcagaaa
     2821 tacgcggcga ttgtccccag tgaccgcata atgcccattt caccagacct tcttgtgcca
     2881 gcggactaat accacggtcg gcgcgatcgc caagccctgt tggactaata atcgataaat
     2941 tacgtggtgt ttgagtctgt ttatatttat cagcaagagc agtaattaac gtggtggctt
     3001 ccagaatacc gccgccagcg cctaacacac aaagtgttgc ttcgtcggga atataattca
     3061 ccgcttcctg tgccgacagg accggcactc gtccattaat acgaggtggt tttacaggtt
     3121 tcattgttaa ttcctgattc aggtgtgatt ttccctacgc cagatggaga attagcgcac
     3181 agagactcac gaaatatttt tattaaatac ggttaaaaat actcttttta gttcggcggg
     3241 ggagagtatt cccgccgaaa tattattgct tatgcctgat gtaaaatagt taataccgta
     3301 cgcaaatcat ttaccgagat ttgccctggc gcagaggctt tttttaccgc accaaaagtt
     3361 gccgccgacc caaatacttc accagccaga cgagaaatta cgccagtttt tgccatcgac
     3421 atcgtgatga ttggacgatc ggcatactgt tcctgcatct ccagggtcgc ggcaagcaac
     3481 gtcagcacat cgctggtact ttgcggcatc agcgcaatct taggaatatc ggcgtcgaag
     3541 gactgcattt tgcgcagacg ggcaatgatt tcttcggctt ccggcgtttt atggaagtca
     3601 tggttggaca tgacaacttt cacatcatgc gcgtgggcgt aggcgacggt ttctttaacc
     3661 tgatcatcgc cggtaaataa ctccagatcg atcatatcaa ccaggccact gtcgatggct
     3721 gcacgattga gtgcaatata agcctcggtg gaaatcgcct gctcgccgcc ttctttggca
     3781 ctgcggaagg taaacagcag cggtttttct ggcatggttt cacggagaat ttttgctgcc
     3841 gctatgacag actccacatt ggagagatcg gcatagtggt ccacacgcca ttccagaata
     3901 tcaaagtccg cttcacgata ggcgagagct tcggatttca cgctggcgat atctttcgcc
     3961 atcagcgaga cgatgatttt aggtgcgccc gtaccaatga cgagatcttt tacagttacg
     4021 gttttcattt tttacccttt ctgcacgcgg tcagcctgtc aggcaccgaa ccccatgacc
     4081 tgtttaacat attccagagg gaaatctttg ccagtccata acgtgaactg ttcagcccct
     4141 tgccacaaca acatgccgta tccatcaatc gttttgcaac ccgcttgttg cgcctgctgc
     4201 aataacttcg tcatatgcgg gttatacacg cattcagtga ccagaagtcc tggatgtaac
     4261 agactgatat cattaaccaa tgattcattc tcaaggggtt tcatacccac tttggtgcca
     4321 ttggttaaaa tgtcggcgga agccagggct tcagcaaagg cttgctgatc ggcgagatcg
     4381 gtgactgtca cgacgcaatc ggtgttttcg ttaacccgct gcgcgaaggc gagagcttta
     4441 tcgaagaact catcccgacg gttaaagagt ttaatttctt ttaaaccttc aattgccccc
     4501 tgcgcgccaa ttgccgttga ggcaccaccg gcccctaata gcaccatcgt tttgcctttg
     4561 atatcaaaac cgctctcttt aatggcgcga atatgacccg tgccgtcggt gttatagcca
     4621 cgcagatagc catcatcatt aacgatggtg ttgatggcac ccaccagttt ggccgctggc
     4681 gttaattcat caacatattc acacgccagt tgtttgttcg gcattgatac accagttccg
     4741 cgcattttga gggcttttaa tccttcaatt gctccgggaa aactatcgtt atccacttca
     4801 aaggccatat aggtaaatgg caatcccgct ttttctaagg ctttattctg catttcgggc
     4861 gataaactgt ggcggatggg ataggccatc aacccaatca attcgtattt tgcggtaaca
     4921 tccataatta actcctttaa cctctatgct taattgtgtt taaccgggtg ctaaatttat
     4981 gctcgccaaa ccgcacatca ttttcaggaa taatgaaaac gcggtaatag cggataaaaa
     5041 caataattgc ggtaatcagg gccagcagcg cgaaagtaaa atcgagaaca atgatatatt
     5101 gcaggccgat gttcgacaga taaccggtaa tcagcggaat aacaaagtta gccagtccac
     5161 ccatcatcat atatatactg gtgactttgg ctttgctttt tgggaaaaac tctgacatga
     5221 ccgaaacgcc gagctgtaaa atgccgccag ctgctgagaa accgataaca aaggctccgg
     5281 cattacacac caatggagaa gggtacaggt agataatggc agctgttatt gttgccagtg
     5341 cagagttaaa tacattagcc cagatgggcc ggaccatttt tttcagcagt gcggcaaaaa
     5401 taaagacaca gaccagcgaa cccatactgt aataagagat ggtttttaat gcctcagctt
     5461 ctgacatacc agcaaaagcc atcgcatatt tgggcatcca caccacaatc acataaaagg
     5521 tcgagaatgc ggctacaccg aacagtactg atgaaacacc ttccagccag actaacggtt
     5581 tgctgttcat ttgcggtaat tcattagcta cgctggcgtc caccaactgg ctggggaatt
     5641 tgcttttcaa cagcatcagc gtgatcagta caaacagaat acccggaata atcagcccat
     5701 agccgtacca gatgttattg agcaacatat agctcaccag cattgggtag aacatttgcc
     5761 cgaatgacac catcgcttta accagtatga ccgccgaacc agaggctttc ggaaagcatt
     5821 ccataagcgc ggggtagcca cccgtatcca gcgctgagtt agcgatacct acgcacactg
     5881 ccagacagta ggcgagagtt aaattcgggc aagcaggaat accaaagaag aatagcagat
     5941 acattattac tgccattaat atcaccgccc gacgaccaaa cttatcggag atcactccga
     6001 agaataaaat actgatcaat cgccccaatc cgataccgga aattaaatag gcaatgcccg
     6061 cgttgtcagt ggaaaacttt tctgccaaag atgtcatgtt ttgggcaagc gtaataacac
     6121 taatgccgtg caggaaatag ctgaagtaaa tacaaagaac agccaggata aatggcgtgc
     6181 tgaaagcctt attttgagac attttcacag ctccttgctg aaagataatt tcgttaccgt
     6241 acgtattgtt ctccgacggt ttacataatt actatactga agataatttt ccctgggtac
     6301 atggcataaa gtttatgttt ccaggaatat tgcatgtcat agtttgtttt tgatcgcgat
     6361 tttccttagt tagcgcagaa agagttattt atccgtaaga tcttgcataa cctgaaaaga
     6421 atattgcatt acccacccgg agcgatattt tcctttagcg tgtgatgccg cgtttctttg
     6481 cgactgcgta agccaataaa cagtgccagt ataaagccga tagcagcgat ggcggtatca
     6541 aaccacataa tatcggcaat acttctttgc gaaagatgag ccgtaatcaa cggaatggta
     6601 aatgttgcaa tgctgcccgc actgtaatag atccccgttg cttttccttt agcgtaggga
     6661 aaacgttccg ccattaacgt caggccaatt tgtacaacac cgcctgcgga ggtaaaaccg
     6721 atgacgaaag caaatattat caccacataa aatgtgggat gcaggcagac ggtgagcagg
     6781 gcgataaatg agataaaggt gtacagcatc agtaatgttg ttgggcgaac ggtattacga
     6841 atgagtggag cagtaataaa tacacacagc agcgaaccca cggtgtagat actgagtaat
     6901 ttgatcgaca tggtgtatga catgccagcg acaaattgtc cgtattgtgc cagccactgg
     6961 ctaaccagat aaaacgttgc cattgagata tagccatata gggaataact ggctaaatcg
     7021 ataattgaac agcgatgttc cgtagagctg gtggttttct ttatgacagg taagtgacga
     7081 cccggatggg gtgggaacgt acaacggtat aaaaacagag cgttaataaa cataatgcct
     7141 gcggcaatca taaaggacca accgaaccac agttcagccc acactaacag gctgataatt
     7201 agcggtaata aaaactgtcc gctggaaaca aatgctttaa ttaaaatatt ggctgtaccc
     7261 ggtgagcgtg gaaaagcttc catcaaactg ggataggtgc ctgcatcgag aaaactgttt
     7321 gccattcccg ccagaaagcc aaaaacataa gcgataatga tgttattggt gtgcaggatg
     7381 ccaaaaaaga aggccatata gcagcacatc ccgagcatga taaaagggcg gcgaccaaag
     7441 cgatcggata ataatcctgc aaaaagcaag acacttaatc gaccaatgcc cagtgatgag
     7501 ataactatcg agacgcccgc ggcattagtc tgccaaagcg tctccagcga ggccatattc
     7561 aggctcatca aaatgacgcc cataccatgc accaggtaat taaaatacaa cccaagtgcg
     7621 gtagggtaat agggattttt cataggttct acttagcaaa ggtgctttaa aaagtgtagc
     7681 gatgttgtgt cagtatccgt tcattattgt tgtaacaacg acaattcctt tctgtggaga
     7741 aattaacaaa gccgctccag atcgtgtgca tataattcag cggctaccga ttgatagatc
     7801 ttccagtcaa taaaattgcc atcagggtag acttgttgaa acgcggttaa gtcggggaaa
     7861 aaacgcatgg tgttgttgcc gatgcggtta tttattttct ctatgatggc atgaagatgt
     7921 ttttttcgtt gctgacgcat agccaacagt tgctcgacta cacaatcagg aatggcgcat
     7981 ttgccatttt cccactgacg ccagctttca ctgtcaccgg tctgggcaat ccaggtcgcg
     8041 cattcatcaa tagtcatggc aaaaatatgg cgaagtgcct ggagttcata agcgttcatc
     8101 gtgtaatggc tgcatgaaat aaacaataaa atataaataa cattatcagc ctgctgacgg
     8161 cttgaagaga gtcacagtat cttgtgcaac attattggaa agagtgaggg gcgaggtagc
     8221 gaagcggaaa aatgttaaaa aaaagcccat cgtgggagat gggcaaagac tacacacagc
     8281 aattcgttgt ttcactcagg ggatttccat gcttataaat caatatgttg atttataacc
     8341 gtgagctaat agtaggcata cggacttttt gcgtcgatca gattcgtctc attagtttaa
     8401 aaactgtaaa gaatttgcga caggtactac cagctgaata gcggtaagca ggaaaatatg
     8461 gggaagagca agcaaatatc aattagcagg ggttaacaat caggcggcag agccgcctga
     8521 tgagaaatta cttattcggt ttctcgattt cgccaagttc ttcgagaatt tcttccggtt
     8581 ggtccgtcgg cggtgggact tcttccaccc acgcggcaaa cagacgccag gaaacggcta
     8641 acagaaccgg accaataaac agaccgatca tcccgaaagc aatcaaacca ccaataacgc
     8701 cagagaggat caggatcagc ggtaaatcgg cacccatgcg aattaacatt gggcggatga
     8761 cgttatcgag cgtgccaacc acaccgctcc aaactaacaa taccgttccc caggtggtat
     8821 cgccagtcca gtagagccag ataatcgccg gaatcagtac cggcaacggt ccaagctgga
     8881 caaggcagga gaggatcatt aacaccgtta gcaaagttgc ataaggtacg ccggatacgg
     8941 cgaggccgat accgccaagc actgcctgta ctaacgccgt caccaccaca cccagcgcca
     9001 ccgcgcggat agcctgtgcc gccagcagca cggcggcatc tccgcgaacg cctgccagac
     9061 gggttgcaaa atggcgaatg ccttgtgcca cctgttcacc gcgccagtac agcagggcgc
     9121 tgaagagaag catcagcgca caatgcacca taaagcgccc gatatgcgcc gcctgcccaa
     9181 cgaaccaggt ggtggtggtg ccaatataag gacggacttt cgccatgatc gccgtccccc
     9241 ccatatccag caaattgtgc cagcctgcat atagcttcgc gccaatcacc ggaatggtat
     9301 taagccacgc cagatcgggt aacgtcatgt caccactgga aatggcttta attagcgggc
     9361 cgctgccgtc gacgatactg ttaaccagca aagcaatagg gatgataaac accatcacta
     9421 ataacagcgt catcaccaga acggcgagag agcggcgacc aaacatgatc ttttgcaaac
     9481 gtaacaatac cggccaggtg gcgataacca ctgtaccggc ccatgcaaag ccgagaataa
     9541 agggttgtac gatccacaga catgccacaa tcatgatggc taaaaacagc accgaaagca
     9601 gaatttgtgc gacatccctg ggctgacgaa catttaccat aactactttt cacctttttt
     9661 gtgcgtcaaa tcgtcgacgc gacgtgaaca cgcgaaactc acctgcataa tgatgagcaa
     9721 tttcagcggt ttttaacagg tcgattctgc ccttaattct gatgggcgtg taagaaaaaa
     9781 atgtggtaca acaactcagg caacacgcaa acgattaatc tctcatcaca gaccgcagga
     9841 aagggtcaat ataatgattc cacagatttc ccaggcaccc ggcgtcgttc aactggtgct
     9901 taattttttg caagagctgg agcaacaggg ttttaccggc gatacggcga caagttatgc
     9961 cgatcgtctg acaatgtcga ctgacaacag tatttaccaa cttctccccg atgcggtggt
    10021 atttccgcgt tcaaccgcag atgtagcgct gatcgcccgt cttgccgcgc aggaacgcta
    10081 ttcatcgttg atctttaccc cccgcggcgg cggcaccggc actaacggac aggcgctcaa
    10141 ccaggggatt attgttgata tgtcccgcca tatgaaccgc atcatcgaaa ttaaccctga
    10201 agagggctgg gtgcgcgttg aggccggggt gattaaagac caactcaatc agtacctgaa
    10261 accctttggc tacttttttg cgccagaact ttcgaccagc aaccgggcaa cgctcggcgg
    10321 gatgatcaat accgatgcat ccggtcaggg atcgctggtc tatggcaaaa cgtcagatca
    10381 cgtacttggc gtacgcgcgg tgttgttggg gggcgatatt ctcgatacgc aacctttacc
    10441 cgtcgaactg gcggaaacgc tgggtaaatc caataccaca atcgggcgaa tttataacac
    10501 ggtttatcaa cgttgccgtc agcaacgcca gttaatcatc gacaacttcc ccaagcttaa
    10561 ccgctttctt accggttacg atctgcgtca tgtctttaac gatgagatga ccgagttcga
    10621 cctgacgcgc attctgacgg gttcagaagg gacgctggcc tttattaccg aagcgcgact
    10681 ggatattacg cgcttgccta aagtacgccg tctggtgaac gtcaaatatg actcttttga
    10741 ctccgcgctg cgtaacgcgc cgtttatggt tgaggcgcgg gcgctttcgg tagagacggt
    10801 ggactcaaaa gtgctgaatc tggcgcggga agatattgtc tggcattccg tcagcgagtt
    10861 gattaccgat gtgcctgata aagagatgct cgggctgaac attgtggaat ttgccggtga
    10921 tgatgaggca ctgattgacg agcgagttaa tgcactctgt gtgcggcttg atgagctgat
    10981 tgccagccaa caggcaggag tgatcggctg gcaggtgtgc cgcgagctgg cgggtgttga
    11041 acgtatctat gcgatgcgca aaaaagccgt tggtctgctt ggcaatgcca aaggtgctgc
    11101 taagccaatt ccgtttgctg aggatacctg cgtaccgccg gaacacctgg cggattatat
    11161 tgctgaattt cgcgcgctgc tcgacagcca cggcttaagc tacggtatgt tcggtcacgt
    11221 cgacgcaggt gtcttgcacg tccgtccggc actggatatg tgcgatccgc aacaagagat
    11281 tttgatgaag caaatctctg atgatgtagt ggcgcttacc gcgaaatacg gtggtttgtt
    11341 gtggggcgaa cacggcaaag gttttcgcgc cgaatacagc ccggcgtttt tcggtgagga
    11401 actttttgca gaactgcgta aagtgaaagc ggcatttgac ccgcataacc gactcaaccc
    11461 cgggaagatt tgcccgccag aaggtctcga tgcgccgatg atgaaagtgg acgcggtgaa
    11521 gcgcggtaca ttcgatcgac agatccccat tgcggtacgc cagcagtggc gtggtgcaat
    11581 ggagtgtaac ggcaacggtt tatgcttcaa ctttgatgct cgtagtccga tgtgtccgtc
    11641 gatgaagatc acccagaacc ggattcattc accgaaaggg cgcgcaacgc tggtacgtga
    11701 atggctgcgt ttgttggcgg atcgcggcgt tgacccactc aaactggaac aagaactgcc
    11761 tgaatccggc gtcagtttgc ggacgttaat tgcccgcacg cgcaatagtt ggcatgcgaa
    11821 taaaggcgaa tatgacttct cacacgaagt caaagaggcg atgtcgggct gtctggcctg
    11881 taaagcttgt tcgactcagt gccccattaa aattgatgtg ccggagtttc gctctcgttt
    11941 cctgcaactc tatcacaccc gttatttacg cccgctgcgc gaccacctcg tcgctacggt
    12001 tgagagttac gcaccgctga tggcacgcgc gccgaaaacc tttaacttct tcattaacca
    12061 gccgctggtg cgcaaactct cggaaaaaca tatcggcatg gttgatttgc cgctgctgtc
    12121 ggtcccctcg ctacaacaac aaatggtggg gcatcgctcg gcaaacatga cgctggaaca
    12181 gcttgaagct ctcaatgcag agcagaaagc gcgcacggtg ctggtggtgc aggacccctt
    12241 taccagctat tacgatgctc aagtggtggc ggattttgtc cgtctggtcg aaaaattagg
    12301 tttccagccc gtgttactgc cattttcgcc aaatggaaaa gcccagcata tcaaaggttt
    12361 tcttaatcgt tttgcgaaga cggcgaaaaa gacggcggat ttcctcaacc gtatggcgaa
    12421 gctgggtatg ccaatggtag gcgtcgaccc ggcgctggta ctttgttatc gcgatgaata
    12481 taaactggct ctgggcgagg agcgtggcga gtttaacgtc ttactggcaa atgaatggct
    12541 ggcaagcgca cttgagtcac agccagtggc tacagtcagc ggtgaatcat ggtatttctt
    12601 tggtcactgt accgaagtta ccgccttgcc gggcgcgcca gcacaatggg cggcaatatt
    12661 tgcccgtttt ggcgcgaaac tggaaaatgt cagcgtgggg tgctgcggca tggcagggac
    12721 ttacggacat gaagcgaaaa accataaaaa ttcgctcggg atctatgaat tatcctggca
    12781 tcaggcgatg cagcgactgc cgcgtaaccg ctgtctggcg accggatatt cctgccgtag
    12841 tcaggtaaaa cgggttgaag gcacaggggt acgccatcct gtgcaggctt tactggagat
    12901 tattaaatga tatggaaacg aaaaatcacc ctggaagcac tgaatgctat gggggaagga
    12961 aacatggtgg gattgctgga tattcgcttt gaacatattg gtgatgacac ccttgaagcg
    13021 acaatgccag tagactcacg gacaaagcag cctttcgggt tgctgcatgg aggtgcatcc
    13081 gtggtactgg ccgaaagtat cggttccgtt gccggttatt tatgtaccga aggtgagcaa
    13141 aaagtggttg gtctggaaat caatgctaac cacgtccgct cgacacgaga agggcgggtg
    13201 cgcggcgtat gcaaaccgtt gcatctcggt tcgcgtcacc aggtctggca gattgaaatc
    13261 ttcgatgaga aagggcgttt gtgctgttcg tcacgattga cgaccgctat tttgtgacgt
    13321 tgtcaccaga aaagtgtgac gcatattgct tttggttatt atctgcacag taaacggtgg
    13381 tatactgtgc aggtcttgtc taaaagcgag gatgctatgt ctactcaact tgatcccacc
    13441 caactggcca ttgaattttt acgccgtgac caaagcaatc tttcacctgc ccaatatctc
    13501 aagcgtctga aacaattaga acttgaattt gctgacctgc ttactctctc ttctgctgaa
    13561 ttaaaagaag aaatctactt cgcctggcgt ctgggcgtgc attgatcctg atttaatgtc
    13621 gcgagttcgc caaatcggcc tggattaatc ttcgtccagg ttcgagggcc tttttaatac
    13681 agcctatcta cccgcctgat aacgttcaaa aataatacaa atcgcagttt gtgttaaaac
    13741 agtgggttag ctttatgaga ttatccttat cgccccttca agagctaagc cactgtgagt
    13801 gccggagata agcgccggat ggggtagaaa cccttaagcc tgtgtcgcac agacttaagg
    13861 gttttcttat ttctgaacgc tgcacttttt tacctatctc ttaaatagtc attttcaata
    13921 caactttttc tttttcatca cagccttaat gctccttaga taataatcat tatctaacaa
    13981 tgagatacct aattcttagc gtgcctgtta acccacacat cagggtctat gcttattaaa
    14041 agcaggaaaa aagcatgttt tacgctaaag cccctgcgtt tgctgggttg aactgataat
    14101 cattatcact aacatgctgt tatacgctga aagcgatgaa gtgaggtaaa tcgatggaca
    14161 tgcattcagg aacctttaat ccacaggatt tcgcctggca gggcttaacg ctgacacccg
    14221 cagccgctgt ccacatccgc gagctggtgg caaagcagcc gggtatgatc ggcgtgcgct
    14281 taggcgtgaa gcaaacaggc tgcgcgggct ttggctatgt gctcgacagt gttagcgagc
    14341 cggacaaaga cgatctgctg tttgaacacg acggcgcgaa gctgtttgtc ccgctgcaag
    14401 cgatgccttt tattgatggc acggaagtcg atttcgttcg cgaaggactt aatcagatat
    14461 tcaaatttca caaccctaaa gcccagaatg aatgtggctg tggcgaaagc tttggggtat
    14521 aggcggtact atgtctcgta atactgaagc aactgacgat gtcaaaacct ggaccggcgg
    14581 cccgctgaat tataaagaag gattcttcac ccagttagcc accgatgagc tggcaaaggg
    14641 gataaacgaa gaggtggtgc gcgcaatttc ggcgaagcgt aatgagccgg agtggatgct
    14701 ggagtttcgt ctcaacgcct atcacgcatg gctggagatg gaagaaccgc actggttgaa
    14761 agcgcactac gacaagctga attatcagga ttacagctac tactcggcac catcgtgcgg
    14821 taattgtgac gacaactgcg cgtctgaacc cggcgcggtg cagcaaactg gcgcgaacgc
    14881 ctttttaagt aaagaggtgg aggcggcgtt tgagcagttg ggcgttcccg tgcgggaagg
    14941 caaagaggtg gcagtggatg ccattttcga ctctgtttcg gttgccacta cttatcgcga
    15001 aaaactggcg gagcagggaa ttattttctg ttcctttggc gaggcgatcc acgatcaccc
    15061 ggaactggtg cgtaaatatc tcggcaccgt ggtgccgggg aatgacaact tctttgccgc
    15121 acttaatgcg gcggtagcct ctgacggtac gtttatttat gtgcctaaag gcgtgcgttg
    15181 cccgatggaa ctttccacct attttcgcat taacgcggaa aaaaccgggc agtttgagcg
    15241 caccattctg gtggccgacg aagacagcta cgttagctac attgaaggct gttccgctcc
    15301 ggtgcgtgac agctatcagt tacacgcggc ggtggtggaa gtcatcatcc ataaaaacgc
    15361 cgaggtgaaa tattccacgg tacaaaactg gttccctggc gataacaaca ccggcggtat
    15421 tctcaacttc gtcaccaagc gtgctttgtg cgaaggcgaa aacagcaaaa tgtcatggac
    15481 gcaatcagaa accgggtcag cgattacgtg gaaatatccc agctgcattt tgcgcggcga
    15541 taactccatt ggtgagtttt actcagtggc gctgaccagc ggtcatcagc aagcggatac
    15601 cggcaccaag atgatccaca tcggtaaaaa caccaaatcg accattatct cgaaagggat
    15661 ctctgccgga catagtcaga acagttatcg cggcttagtg aaaatcatgc cgacggcaac
    15721 caatgcgcgc aatttcactc agtgcgactc aatgctgatt ggcgctaatt gtggggcgca
    15781 taccttcccg tatgtcgaat gtcgcaataa cagcgcacaa ctggagcacg aggcaacgac
    15841 atcacgtatt ggtgaagatc aactgtttta ctgcctgcaa cgcgggatca gcgaagaaga
    15901 cgccatctcg atgattgtta acggtttttg caaagacgtg ttctcagaac tgccgctgga
    15961 atttgccgtt gaagcacaaa aactcctcgc catcagtctt gaacacagcg tcggataagg
    16021 aataaacatg ttaagtatta aagatttaca cgtcagcgtg gaagataaag ctatcctgcg
    16081 tggattaagc ctcgacgttc gtcccggcga agttcacgcc attatggggc ccaatggttc
    16141 gggcaaaagt accttatcgg caacgcttgc cgggcgagaa gattatgaag tgacgagcgg
    16201 cacggttgag tttaaaggca aagatttgct tgcgctgtcg ccggaagatc gtgcaggtga
    16261 aggcatcttt atggccttcc agtatccggt ggaaattccc ggcgtcagta accagttttt
    16321 cctgcaaacg gcacttaatg cggtgcgcag ctatcgcggc caggaaacgc tcgaccgctt
    16381 tgattttcag gatttgatgg aagagaaaat cgctctcctg aagatgccgg aagatttatt
    16441 aacccgttcg gtaaacgttg gtttttccgg cggcgagaaa aagcgcaacg atattttgca
    16501 aatggcggtg ctggaaccgg agttatgcat tcttgatgag tcagactccg ggctggatat
    16561 tgacgcatta aaagtggttg ccgatggcgt gaactcgctg cgtgacggca agcgttcatt
    16621 catcattgtt acgcactacc aacgcattct cgactacatc aagcctgatt acgtccatgt
    16681 gctgtatcag ggacgaattg tgaaatccgg cgatttcacg ttggtcaaac aactggagga
    16741 gcagggttat ggctggctta ccgaacagca gtaacgcact gcaacagtgg catcatttgt
    16801 ttgaagctga aggggcgaaa cgctccccgc aagcacagca gcatttacaa caattgctgc
    16861 gtaccggact gccgacacgt aaacatgaaa actggaaata tacgccgctg gacgggctga
    16921 ccaatagcca gtttgtcagc attgcggggg agatatcccc acagcagcgt gatgccttag
    16981 cgttaacatt agacgccgtg cggctggtgt ttgtcgatgg acgttacgtg cccgcactga
    17041 gcgatgcaac tgaaggcagc ggatatgaag tgagcattaa cgacgaccgt caggggttac
    17101 ccgacgctat tcaggcggaa gtgtttctgc atttgacgga aagcctggca caaagcgtga
    17161 cgcatatcgc cgtgaagcgc ggtcaacggc cggcaaagcc attgctgtta atgcatatca
    17221 cccagggcgt ggcaggtgaa gaggtgaata ctgcccatta ccggcatcat ctggatctgg
    17281 cggaaggtgc cgaagcaacg gtgatcgaac attttgtcag ccttaatgat gctcgtcact
    17341 tcaccggcgc acggttcact atcaacgtcg cagcgaacgc ccatttgcag catatcaagc
    17401 tggcgtttga aaacccgctc agttaccact ttgctcataa cgatttgttg ctggctgacg
    17461 atgccaccgc atttagccac agtttcctgc tgggtggcgc agtgttacga cacaacacca
    17521 gtacgcaact caatggcgaa aacagcacgc tgcggatcaa tagcctggcg atgccggtga
    17581 aaaacgaggt gtgtgatact cgtacctggc tggaacacaa taaaggtttt tgtaacagcc
    17641 gacagttgca caaaactatc gtcagcgaca aaggccgcgc ggtatttaac ggtttgatca
    17701 acgtcgcgca gcacgccatc aaaacggatg gtcagatgac caacaataat ctgctgatgg
    17761 gcaaactggc ggaagtggat acgaaaccgc agctggaaat ctatgcagat gatgtgaaat
    17821 gcagccacgg cgcgacggtg gggcgtattg atgacgaaca gatgttttat ctgcgctcgc
    17881 gcgggatcaa tcagcaagat gcccagcaga tgatcattta cgcctttgct gccgaactga
    17941 cggaagcact gcgtgatgag gggcttaaac agcaggtgct ggcccgaatc ggtcaacgac
    18001 tgccaggagg tgcaagatga ctttttccgt cgacaaagtg cgggccgact ttccggtgct
    18061 ttctcgtgag gtaaacggtt tgccgctggc ttatctcgac agtgccgcca gtgcgcagaa
    18121 accaagccag gtgattgacg ccgaggctga gttttatcgt cacggctacg cggcggtgca
    18181 tcgcggtatt cataccttaa gcgcccaggc gaccgagaaa atggagaacg tgcgcaagcg
    18241 ggcatcgctg tttattaatg cccgttcggc ggaagagctg gtgttcgtcc gcggtacgac
    18301 ggaagggatc aatctggtcg ccaatagctg gggcaacagc aatgtgcggg cgggcgataa
    18361 catcatcatc agtcagatgg agcaccacgc taacattgtc ccctggcaga tgctttgtgc
    18421 acgcgttggc gcagagctgc gtgtgatccc gctcaacccc gacggtactc tgcaactgga
    18481 gacgctgcct acgctgtttg atgagaaaac tcgcctgctg gcaattactc atgtctccaa
    18541 cgtgcttggg acagaaaatc cgctggcgga gatgatcaca cttgcgcatc aacatggcgc
    18601 aaaagtgctg gtggatggcg ctcaggcggt gatgcatcat ccggtggatg ttcaggcgct
    18661 ggattgcgat ttttacgtgt tctccgggca taaactgtat ggccctaccg gaattggcat
    18721 tctttatgtc aaagaagcct tgttgcagga gatgccgccg tgggaagggg gcggttctat
    18781 gattgccacc gttagcctga gtgaaggcac tacctggacc aaagcgccgt ggcggtttga
    18841 agccggtacg cccaataccg gaggtattat tggccttggc gcggcgctgg agtatgtttc
    18901 ggcgctgggg cttaacaaca ttgccgagta tgaacaaaat ctgatgcatt acgcattgtc
    18961 gcagttggca gcggtgccgg atctcactct ctatggccca caaaacaggc ttggcgttat
    19021 tgcttttaat ctcggtaaac accacgccta tgatgttggc agttttctcg ataattacgg
    19081 cattgctgtg cgtaccggac atcactgcgc aatgccatta atggcctatt acaacgtccc
    19141 tgcgatgtgt cgggcatcgc tggccatgta taacacccat gaagaagtgg atcgtctggt
    19201 gaccggcctg caacgtattc accgtctgct gggataacag ggaggcacta tggctttatt
    19261 gccggataaa gaaaaattgc tgcgtaattt tttacgctgc gccaactggg aagagaaata
    19321 tctctacatt attgagttgg gtcagcgtct gccagaatta cgtgacgaag acaaaagccc
    19381 gcaaaatagc atccagggct gccagagtca ggtgtggatt gtgatgcgtc aaaatgcgca
    19441 gggaattatt gaattacagg gcgacagcga tgcggccatt gtaaaagggc ttattgcggt
    19501 cgtctttatt ctttacgatc aaatgacgcc gcaggatatt gtcaatttcg atgtgcgccc
    19561 gtggtttgaa aaaatggcgc tcacccaaca tctcacccca tcacgctcac aaggtctgga
    19621 agcgatgatt cgcgcaattc gcgccaaagc cgctgcactt agctaaacta aagggacagc
    19681 tttcatccgg ttggctcaca ttgccgggtg gtcaatgttt acactggcct gcacaggagt
    19741 gttttggttt caggtgaaca taagcgccat ccgacgtttc agcgtgagtc tccggcaaat
    19801 acaggaggtt tacaatgaaa cgcgcgtctt tgcttacact cacgcttatc ggcgctttta
    19861 gcgccatcca ggctgcctgg gcggttgatt atccgctacc accaaccggc agtcgactgg
    19921 ttgggcaaaa tcaaacgtat acggtgcaag aaggggataa aaaccttcag gccatcgccc
    19981 gacgttttga tactgcggca atgttgatcc ttgaagccaa taacactatc gccccggtgc
    20041 caaaacccgg tacgacgata actattcctt cacaactatt attacctgat gcgccgcgtc
    20101 aggggattat tgtaaacctt gcagagctgc gcctttataa ttatccgccg ggagaaaata
    20161 tcgtgcaggt ttatccgata ggtattggtt tgcaggggct ggaaacgccg gtgatggaaa
    20221 cgcgtgtggg gcagaaaatc cctaacccaa cctggacgcc tacggcgggc attcgtcaac
    20281 gttcgctgga gcgtggcatt aaattaccac cagtcgttcc tgccgggccc aataacccac
    20341 taggacgtta cgcactgcgc ctcgcgcatg gtaatggcga atacctcatt catggcacca
    20401 gtgcgccgga cagcgtcggt ttgcgcgtca gttcgggctg tattcgcatg aatgcaccgg
    20461 atattaaagc tttattctcc agcgtgcgga cgggaacgcc ggtgaaagtg atcaacgaac
    20521 cggtgaaata ttccgtggaa cctaacggga tgcgttatgt tgaagtacat cgaccactat
    20581 cggcagaaga acagcagaac gttcagacaa tgccgtacac gctgccagca ggctttacgc
    20641 aatttaaaga caataaggct gtagatcaga agttagtcga taaagcgttg tatcgtcggg
    20701 cagggtatcc ggttgcggtg agcagtggag caactcccgc agccagcaat gcgccttcag
    20761 tagagtcagc gcagaatggg gaaccagagc aagggaatat gttacgcgcg acgcagtagc
    20821 ggtaaatagc aggcaaaaaa atggcgcaca tcgtgcgcca tttttcactt cacaggtact
    20881 attacttgcg gtatttagta gccatgttgt ccagacgctg gttagcacga gctgcgtcat
    20941 ctttagcagc ctgaacgtcg gaacgcattg cgttcacgtc gttgctcagc tggtcaactt
    21001 tagcgttcag agtctgaacg tcagaagaca gctgatcgat tttagcgttg ctggagcaac
    21061 ctgccagcag agtagaaccc aggattaccg cgcccagtac cagtttagta gctttcatta
    21121 ttaataccct ctagattgag ttaatctcca tgtagcgtta caagtattac acaaagtttt
    21181 ttatgttgag aatatttttt tgatgggaag gcacttattt ttgatcgttc gctcaaagaa
    21241 gcatcgaacg ctgggtttta gataaaaaaa ttgagagaaa acaggatgct tccatcggat
    21301 tcatcttaga taaagtgaga ttatatagtg aaatccgatt tgatttttta ttgcttctgg
    21361 ttatcgatta aataaaaaaa gcgcccatca gggcgcttcg atatacaaat taattcacaa
    21421 aagcaatatt acaggacgtg aacagatgcg gtgttagtag tgccgctcgg taccagtgca
    21481 ccagaaacca taactacgac atcacctttg tgtgccagac cgctctgcag agccagttct
    21541 ttacccagac ggtagaagtc atcagtagaa gtgatctctt taaccagctg cggcacaaca
    21601 cctttgctca gtactaactg gtgagccgtt ttttcgttgg tggtcagtgc caggatggtg
    21661 gcatccggga agtatttacg tactgcgcga gcagatttac cgccctgggt agcaaccacg
    21721 atcagcgggg catccagttt ttcagcagtt tcaacggcac cacggcatac cgcttcggta
    21781 atacgcagtt tacggttgtc attgttgaac tcgagacggc tgttcatcac gcggtcggta
    21841 cgttcgcaga tggtcgccat gatagaaacc gcttccagcg ggtatttacc ttttgcggat
    21901 tcaccagaca gcatcactgc gtcagtaccg tcgaggatgg cgtttgcaac gtcaccggct
    21961 tctgcgcgag tcgggcgtgg gtttttgatc atggaatcca gcatctgggt cgcagtgata
    22021 acaactttac gtgcacggat acatttttcg atcatcatct tttgggcgaa gataacttct
    22081 tctaccggga tttctacacc caggtcgcca cgcgcaacca tgatgccgtc agaggcttcg
    22141 aggatttcgt cgaagttgtt gaggccttcc tggttttcga ttttggagat gatgtggatg
    22201 ttttcgccgc cgtgcgcttt caggtgctca cggatttcga taacgtcaga acgcttacga
    22261 ataaaggaag cagcaacaaa gtctacgcct tgttcgcaac caaagatcag gtcctgtttg
    22321 tctttttcag ccagtgctgg cagggcaatg gaaacgccag gcaggttcac acctttgttt
    22381 tcgcccaggt cgccgttgtt cagcacttta cagataactt tgttaccttc aatggcggta
    22441 acttccatac cgatcagacc gtcgtcaacc agtacggtgt tgccaacaga caggtcagta
    22501 gtgaaacctt catacgttac cgcaaccatt tcgctgttgc cgataacaga tttatcagta
    22561 gtgaaagtaa aggtctgacc cgctttcaga gaaacgtcgt taccgccttc cagtttcatg
    22621 gtgcggattt ctggaccttt ggtatcaagc aggatagcgg cggttttacc agttttgctc
    22681 atcacgttgc gcagattctg aatgcgctga ccgtgttctg catagtcacc atgagagaag
    22741 ttcagacgca taacgttcat gccagcgtcc agcattttag ctaacatctc ttcagattcg
    22801 gttttcggtc cgatggtgca aacaattttg gtctttttca tgacagtctt agtctttaag
    22861 ttgagaagga tgggagaaac ttgctttctg ggcgcactgc cggaaagcga atccggtatt
    22921 acgaaagtgg ttgtgcctcg ctctaaggat aggtgacatc gaaaaagcgt gcagaggaat
    22981 gtgtgcttgt gtctcagccc aacgtgagtg cctgtttttg agtcgttgag ttctacagtc
    23041 caaagtgctg aaaccattca agagtcaatt ggcgcgcatt atacgctgaa attaatccaa
    23101 aaggaaaaca aaaacagcgt ttcaaaaaat atacaacttg ttagacaata aattgttatc
    23161 aaagattcag cgttccaggg aaaaggttac gcaaaaactt cgttgtttgc gcaacgtctg
    23221 g
//
LOCUS       DADSYM010000071        23147 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2415
            SAMN08039579-rid11723173.denovo.071, whole genome shotgun sequence.
ACCESSION   DADSYM010000071 DADSYM010000000
VERSION     DADSYM010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039579
            Sequence Read Archive: SRR6362533
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 23147)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23147)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980162.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 03:39:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,484
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 4,347
            CDSs (with protein)               :: 4,347
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 43 of 85
            Pseudo Genes (incomplete)         :: 37 of 85
            Pseudo Genes (internal stop)      :: 19 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23147
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039579-rid11723173.denovo.071"
                     /strain="LGE2415"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8302 N 95.9628 W"
                     /collection_date="2015-08-24"
     gene            142..1062
                     /gene="malM"
                     /locus_tag="J1Y83_002391"
     CDS             142..1062
                     /gene="malM"
                     /locus_tag="J1Y83_002391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose operon protein MalM"
                     /protein_id="HBA7630535.1"
                     /translation="MKMNKSLIALCLSAGLLASAPGISLADVNYVPQNTSDAPAIPSA
                     ALQQLTWTPVDQSKTQTTQLSTGGQQLNVPGISGPVAAYSVPANIGELTLTLTSEVNK
                     QTSVFAPNVLILDQNMTPSAFFPSSYFTYQEPGVMSADRLEGVMRLTPALGQQKLYVL
                     VFTTEKDLQQTTQLLDPAKAYAKGVGNSIPDIPDPVARHTTDGLLKLKVKTNSSSSVL
                     VGPLFGSSAPAPVTVGNTAAPAVAAPAPAPVKKSEPMLNDTESYFNTAIKNAVAKGDV
                     DKALKLLDEAERLGSTSARSTFISSVKGKG"
     gene            1241..1738
                     /gene="ubiC"
                     /locus_tag="J1Y83_002392"
     CDS             1241..1738
                     /gene="ubiC"
                     /locus_tag="J1Y83_002392"
                     /EC_number="4.1.3.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate lyase"
                     /protein_id="HBA7630536.1"
                     /translation="MSHPALTQLRALRYFTEIPALEPQLLDWLLLEDSMTKRFEQQGK
                     TVSVTMIREGFVEQNEIPEELPLLPKESRYWLREILLCADGEPWLAGRTVVPVSTLSG
                     PELALQKLGKTPLGRYLFTSSTLTRDFIEIGRDAGLWGRRSRLRLSGKPLLLTELFLP
                     ASPLY"
     gene            1751..2623
                     /gene="ubiA"
                     /locus_tag="J1Y83_002393"
     CDS             1751..2623
                     /gene="ubiA"
                     /locus_tag="J1Y83_002393"
                     /EC_number="2.5.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybenzoate octaprenyltransferase"
                     /protein_id="HBA7630537.1"
                     /translation="MEWSLTQNKLLAFHRLMRTDKPIGALLLLWPTLWALWVATPGVP
                     QLWILAVFVAGVWLMRAAGCVVNDYADRKFDGHVKRTANRPLPSGAVTEKEARALFVV
                     LVLISFLLVLTLNTMTILLSIAALALAWVYPFMKRYTHLPQVVLGAAFGWSIPMAFAA
                     VSESVPLSCWLMFLANILWAVAYDTQYAMVDRDDDVKIGIKSTAILFGQYDKLIIGIL
                     QIGVLALMAIIGELNGLGWGYYWSILVAGALFVYQQKLIANREREACFKAFMNNNYVG
                     LVLFLGLAMSYWHF"
     gene            complement(2778..5201)
                     /gene="plsB"
                     /locus_tag="J1Y83_002394"
     CDS             complement(2778..5201)
                     /gene="plsB"
                     /locus_tag="J1Y83_002394"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418465.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsB"
                     /protein_id="HBA7630538.1"
                     /translation="MSGWPRIYYKLLNLPLSILVKSKSIPADPAPELGLDTSRPIMYV
                     LPYNSKADLLTLRAQCLAHDLPDPLEPLEIDGTLLPRYVFIHGGPRVFTYYTPKEESI
                     KLFHDYLDLHRSNPNLDVQMVPVSVMFGRAPGREKGEVNPPLRMLNGVQKFFAVLWLG
                     RDSFVRFSPSVSLRRMADEHGTDKTIAQKLARVARMHFARQRLAAVGPRLPARQDLFN
                     KLLASRAIAKAVEDEARSKKISHEKAQQNAIALMEEIAANFSYEMIRLTDRILGFTWN
                     RLYQGINVHNAERVRQLAHDGHELVYVPCHRSHMDYLLLSYVLYHQGLVPPHIAAGIN
                     LNFWPAGPIFRRLGAFFIRRTFKGNKLYSTVFREYLGELFSRGYSVEYFVEGGRSRTG
                     RLLDPKTGTLSMTIQAMLRGGTRPITLIPIYIGYEHVMEVGTYAKELRGATKEKESLP
                     QMLRGLSKLRNLGQGYVNFGEPMPLMTYLNQHVPDWRESIDPIEAVRPAWLTPTVNNI
                     AADLMVRINNAGAANAMNLCCTALLASRQRSLTREQLTEQLNCYLDLMRNVPYSTDST
                     VPSASASELIDHALQMNKFEVEKDTIGDIIILPREQAVLMTYYRNNIAHMLVLPSLMA
                     AIVTQHRHISRDVLMEHVNVLYPMLKAELFLRWDRDELPDVIDALANEMQRQGLITLQ
                     DDELHINPAHSRTLQLLAAGARETLQRYAITFWLLSANPSINRGTLEKESRTVAQRLS
                     VLHGINAPEFFDKAVFSSLVLTLRDEGYISDSGDAEPAETMKVYQLLAELITSDVRLT
                     IESATQGEG"
     gene            5372..5740
                     /locus_tag="J1Y83_002395"
     CDS             5372..5740
                     /locus_tag="J1Y83_002395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HBA7630539.1"
                     /translation="MANNTTGFIRIIKAAGYSWKGLRAAWINEAAFRQEGVAVLLAVV
                     IACWLDVDAITRVLLISSVMLVMIVEILNSAIEAVVDRIGSEYHELSGRAKDMGSAAV
                     LIAIIVAVITWCILLWSHFG"
     gene            5850..6458
                     /gene="lexA"
                     /locus_tag="J1Y83_002396"
     CDS             5850..6458
                     /gene="lexA"
                     /locus_tag="J1Y83_002396"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000646075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="repressor LexA"
                     /protein_id="HBA7630540.1"
                     /translation="MKALTARQQEVFDLIRDHISQTGMPPTRAEIAQRLGFRSPNAAE
                     EHLKALARKGVIEIVSGASRGIRLLQEEEEGLPLVGRVAAGEPLLAQQHIEGHYQVDP
                     SLFKPNADFLLRVSGMSMKDIGIMDGDLLAVHKTQDVRNGQVVVARIDDEVTVKRLKK
                     QGNKVELLPENSEFKPIVVDLRQQSFTIEGLAVGVIRNGDWL"
     gene            6477..7856
                     /gene="dinF"
                     /locus_tag="J1Y83_002397"
     CDS             6477..7856
                     /gene="dinF"
                     /locus_tag="J1Y83_002397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709876.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter DinF"
                     /protein_id="HBA7630541.1"
                     /translation="MPPCVAVCFSSLFIRLVCMAFLTSSDKALWHLALPMIFSNITVP
                     LLGLVDTAVIGHLDSPVYLGGVAVGATATSFLFMLLLFLRMSTTGLTAQAYGAKNPQA
                     LARALVQPLLLALGAGALIALLRTPIIDLALHIVGGSEAVLEQARRFLEIRWLSAPAS
                     LANLVLLGWLLGVQYARAPVILLVVGNILNIVLDVWLVMGLHMNVQGAALATVIAEYA
                     TLLIGLLMVRKILKLRGISGEMLKTAWRGNFRHLLALNRDIMLRSLLLQLCFGAITVL
                     GARLGSDIIAVNAVLMTLLTFTAYALDGFAYAVEAHSGQAYGARDGSQLLDVWRAACR
                     QSGIVALLFSVVYLLAGEHIIALLTSLTQIQQLADRYLIWQVILPLVGVWCYLLDGMF
                     IGATRAAEMRNSMAVAAAGFALTLLTLPWLGNHGLWLALTVFLALRGLSLAAIWRRHW
                     RNDTWFVAT"
     gene            7972..8181
                     /locus_tag="J1Y83_002398"
     CDS             7972..8181
                     /locus_tag="J1Y83_002398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HBA7630542.1"
                     /translation="MNKDKAGGNWKQFKGKVKEQWGKLTDDDMTIIEGKRDQLVGKIQ
                     ERYGYQKDQAEKEVDSWEKRHDYRW"
     gene            complement(8223..8738)
                     /gene="zur"
                     /locus_tag="J1Y83_002399"
     CDS             complement(8223..8738)
                     /gene="zur"
                     /locus_tag="J1Y83_002399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418470.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc uptake transcriptional repressor Zur"
                     /protein_id="HBA7630543.1"
                     /translation="MEKTTTQELLAQAEKICAQRNVRLTPQRLEVLRLMSLQDGAISA
                     YDLLDLLREAEPQAKPPTVYRALEFLLEQGFVHKVESTNSYVLCHLFDQPTHTSAMFI
                     CDRCGAVKEECAEGVEDIMHTLAAKMGFALRHNVIEAHGLCSACVEVEACRHPEQCHH
                     DHSIQVKKKPR"
     gene            8894..9889
                     /gene="dusA"
                     /locus_tag="J1Y83_002400"
     CDS             8894..9889
                     /gene="dusA"
                     /locus_tag="J1Y83_002400"
                     /EC_number="1.3.1.-"
                     /EC_number="1.3.1.91"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016237616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(20/20a) synthase DusA"
                     /protein_id="HBA7630544.1"
                     /translation="MPAESHTVYPAYRFSIAPMLDWTDRHCRYFLRLLSRNTLLYTEM
                     VTTGAIIHGKGDYLAYSEEEHPVALQLGGSDPAALAQCAKLAEARGYDEINLNVGCPS
                     DRVQNGMFGACLMGNALLVADCVKAMRDVVSIPVTVKTRIGIDDQDSYEFLCDFINTV
                     SGKGECEMFIIHARKAWLSGLSPKENREIPPLDYPRVYQLKRDFPHLTMSINGGIKSL
                     EEAKAHLQHMDGVMVGREAYQNPGILAAVDREIFGSSDTDADPVAVVRAMYPYIEREL
                     SQGTYLGHITRHMLGLFQGIPGARQWRRYLSENAHKAGADINVLEHALKLVADKR"
     gene            10023..10265
                     /gene="pspG"
                     /locus_tag="J1Y83_002401"
     CDS             10023..10265
                     /gene="pspG"
                     /locus_tag="J1Y83_002401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313059.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response protein PspG"
                     /protein_id="HBA7630545.1"
                     /translation="MLELLFVIGFFVMLMVTGVSLLGIIAALIVATAFMFLGGMLALM
                     IKLLPWLLLAVAVVWVIRAIKAPKVPKYQRYDRWRY"
     gene            complement(10430..11413)
                     /locus_tag="J1Y83_002402"
     CDS             complement(10430..11413)
                     /locus_tag="J1Y83_002402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone oxidoreductase"
                     /protein_id="HBA7630546.1"
                     /translation="MATRIEFHKHGGPEVLQAVEFTPADPAENEIQVENKAIGINFID
                     TYIRSGLYPPPSLPSGLGTEAAGIVSKVGSGVKHIKAGDRVVYAQSALGAYSSVHNIN
                     ADKAAILPAAISFEQAAASFLKGLTVYYLLRKTYEIKPDEQFLFHAAAGGVGLIACQW
                     AKALGAKLIGTVGTAQKAQSALKAGAWQVINYREENLVERLKEITGGKKVRVVYDSVG
                     RDTWERSLDCLQRRGLMVSFGNSSGAVTGVNLGILNQKGSLYVTRPSLQGYITTREEL
                     TEASNELFSLIASGVIKVDVAEQQKYPLKDARRAHEILESRATQGSSLLIP"
     gene            11496..12911
                     /locus_tag="J1Y83_002403"
     CDS             11496..12911
                     /locus_tag="J1Y83_002403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase DnaB"
                     /protein_id="HBA7630547.1"
                     /translation="MAGNKPFNKQQAEPRERDPQVAGLKVPPHSIEAEQSVLGGLMLD
                     NERWDDVAERVVADDFYTRPHRHIFTEMARLQESGSPIDLITLAESLERQGQLDSVGG
                     FAYLAELSKNTPSAANISAYADIVRERAVVREMISVANEIAEAGFDPQGRTSEDLLDL
                     AESRVFKIAESRANKDEGPKNIADVLDATVARIEQLFQQPHDGVTGVNTGYDDLNKKT
                     AGLQPSDLIIVAARPSMGKTTFAMNLVENAAMLQDKPVLIFSLEMPSEQIMMRSLASL
                     SRVDQTKIRTGQLDDEDWARISGTMGILLEKRNIYIDDSSGLTPTEVRSRARRIAREH
                     GGIGLIMIDYLQLMRVPALSDNRTLEIAEISRSLKALAKELNVPVVALSQLNRSLEQR
                     ADKRPVNSDLRESGSIEQDADLIMFIYRDEVYHENSDLKGIAEIIIGKQRNGPIGTVR
                     LTFNGQWSRFDNYAGPQYDDE"
     gene            12964..14043
                     /gene="alr"
                     /locus_tag="J1Y83_002404"
     CDS             12964..14043
                     /gene="alr"
                     /locus_tag="J1Y83_002404"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HBA7630548.1"
                     /translation="MQAATVVINRRALRHNLQRLRELAPASKMVAVVKANAYGHGLLE
                     TARTLPDADAFGVARLEEALRLRAGGITKPVLLLEGFFDARDLPTISAQHFHTAVHNE
                     EQLAALEEASLDEPVTVWMKLDTGMHRLGVRPEQAEAFYHRLTQCKNVRQPVNIVSHF
                     ARADEPKCGATEKQLAIFNTFCEGKPGQRSIAASGGILLWPQSHFDWVRPGIILYGVS
                     PLEDRSTGADFGCQPVMSLTSSLIAVREHKAGEPVGYGGTWVSERDTRLGVVAMGYGD
                     GYPRAAPSGTPVLVNGREVPIVGRVAMDMICVDLGPQAQDKAGDPVILWGEGLPVERI
                     AEMTKVSAYELITRLTSRVAMKYVD"
     gene            14296..15489
                     /gene="tyrB"
                     /locus_tag="J1Y83_002405"
     CDS             14296..15489
                     /gene="tyrB"
                     /locus_tag="J1Y83_002405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transaminase"
                     /protein_id="HBA7630549.1"
                     /translation="MFQKVDAYAGDPILTLMERFKEDPRCDKVNLSIGLYYNEDGIIP
                     QLKAVADAAARLNAQPHGASLYLPMEGLNSYRHAIAPLLFGADHPVLQQQRVATIQTL
                     GGSGALKVGADFLKRYFPESGVWVSDPTWENHVAIFAGAGFEVSTYPWYDEATNGVRF
                     NDLLPTLKTLPARSIVLLHPCCHNPTGADLTNDQWDAVIEILKARELIPFLDIAYQGF
                     GAGMEEDAYAIRAIASAGLPALVSNSFSKIFSLYGERVGGLSVLCEDAEAAGRVLGQL
                     KATVRRNYSSPPNFGAQVVAAVLNDEALKASWLAEVEEMRTRILAMRQELVKVLSTEM
                     PERNFDYLLNQRGMFSYTGLSSAQVDRLREEFGVYLIASGRMCVAGLNAQNVHRVAKA
                     FAAVM"
     gene            15671..18415
                     /locus_tag="J1Y83_002406"
     CDS             15671..18415
                     /locus_tag="J1Y83_002406"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001468317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HBA7630550.1"
                     /translation="MENMTSPGTLLSGDNATWLEEYYQTWLRTPEKLPEDWRRFFLSP
                     ELTVQSVSGDNNVSGATLKKQAAVVQLINAWRTQGHLRAKLDPLGLNPPADVPSLQPG
                     FWGLSEEDLLQEFSVTFGAHTTQMPLKQLLNLLEQAWAGSQAYELAHLENREEINWLL
                     SRIESSNALQADVQTCIARFEKLMAAETLERYLHTRYVGQKRFSLEGGESAIPALDTL
                     TKRLRAQGVEEMVIGMAHRGRLNVLVNLLNKDPAQLFAEFEGKQTIGSGSGDVKYHMG
                     YSSNLETPAGSLHVALAYNPSHLEIVNPVVLGQVRARQERRGEDGQAKVVGVLIHGDS
                     ALGGLGVNQTTFNLSQTQGYGTGGTLHLVINNQIGFTTSRLQDMRSSRYCTDIAKMVA
                     APIIHVNGDDVDAVCQVMELACEWRDTFRRDIIIDICCFRKHGHNESDEPRLTQPQMY
                     QAVDVHPGTLARYGESLARRGLLTQAQQDEMTARYRDWLDSCQKREPQPLKPAIHSFS
                     ANWYGLTNPHWSAPVSTALPRQKLVAYGEIISTLPPDVVAHPTIKRQLALRQDMAAGT
                     QPVDWGMAEMLAYASLVDAGVGVRLSGEDSGRGTFSHRHAVVHHQTEARRYLPLQHIR
                     AGQASFDVYDSVLNEEALLAFEYGYSTSAPQQLVIWEAQFGDFANGAQVAIDQFISSG
                     ETKWDRYSGLTILLPHGYDGQGPEHSSARPERWLQLCAENNMQVVMPSESAQMFHLLR
                     GQALRPMRKPLVIMMSKRLLRFKGAMSELCEFTDGAYKPVITDPQLHQSQKVKRVILC
                     SGQVYYDVLEARKQREHEDEVAIVRLEQLYPFPVAELNDVLASWPNCCEWIWLQEEPE
                     NQGAWRQIRHELAALKINTPYWQYAGRPAAAAPATGYGRVHKQQIDEFLAAAFADIQP
                     "
     gene            18448..19602
                     /gene="odhB"
                     /locus_tag="J1Y83_002407"
     CDS             18448..19602
                     /gene="odhB"
                     /locus_tag="J1Y83_002407"
                     /EC_number="2.3.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001468318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase complex
                     dihydrolipoyllysine-residue succinyltransferase"
                     /protein_id="HBA7630551.1"
                     /translation="MIEITVPVLPESVTEGTLTTWCKQEGEHVKRDDVIAELETDKVI
                     LEIPAPHDGVLSNIIVSEGSTVTSAQLLAHLKPQAVIEETVTPVTETLAMPSARLEAQ
                     RSGVELADVAGSGRNGHILKEDVQRVTPAPVIQPERVAEIAPAKPLTPGARQERREPM
                     SRLRQRIAERLLASQQNNAILTTFNEVNMQSVMDLRARWKDRFAEKHGVKLGFMSFFV
                     KAVTRALERFPVVNASVDGNEIIWRDYCDIGIAVSSNRGLVVPVLRNAQSLSLVEIER
                     QIAEYATQARNGKLPLEALQGGTFSITNGGTFGSMMSTPIINPPQSAILGMHAITPRP
                     VAENGQVVIRPMMYLALSYDHRIIDGQEAVQTLVAIRELLESPEQLLLDL"
     gene            19614..21032
                     /gene="lpdA"
                     /locus_tag="J1Y83_002408"
     CDS             19614..21032
                     /gene="lpdA"
                     /locus_tag="J1Y83_002408"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000103914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HBA7630552.1"
                     /translation="MSTIFDVAVMGGGPGGYVAALRAAQNGLSVVCIDDGVNAQGEPS
                     PGGTCLNVGCIPSKSLLQSSELYAQVQHEASIHGVNVDGVSFNAAAMIQRKDAIVSRL
                     TMGIGLLFKKNKVKYLCGLATLEHARDEIWQLRVNDQQIRARNVVIATGSQPRQLPGV
                     TIDNQQILDNRGALALTAVPPRLGVIGAGVIGLELGSVWNRVGSDVTLLEMAPTFLPA
                     LEARLSNEVRKAMIASGMKMQLAVEIEAIEQRDDGVHVRWRQGEKREESRFDKLILAI
                     GRVPRLSGVDLVQLGLEADNRGGIAVDNLCRTGKAGLWAIGDVVRGPMLAHKAMAEGV
                     VVADQIAGLAVEPINFALIPSVIYTQPEVAWVGENEASLKAAGRVFNKGNSLFAGNGR
                     ALALGQEGGRCTLYSDKHTDRVLGGAIVGPQASELINEIALAMTFSASGEDIACAIHA
                     HPTLSEVIHEAAMALNNKALHG"
     gene            21054..22223
                     /gene="sucC"
                     /locus_tag="J1Y83_002409"
     CDS             21054..22223
                     /gene="sucC"
                     /locus_tag="J1Y83_002409"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001048620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HBA7630553.1"
                     /translation="MNLHEYQAKSLLAGMGMPCPKEIAIQQISQLADAWQHIACPSKG
                     AVLKAQVHAGGRGKAGGVKVLKQLPEAQAFVQQMLGSQLVTYQTGPEGQYVSSILLCE
                     NIYPVRQELYFGMVVDRESQRVTFIVSPEGGVEIEKVAHETPEKISSVSIDPLTGVQP
                     CHIREMFAVLQLEHGLFAAFSRLVNQAWKAFNELDFALLEINPLVLRETGEFMCADAK
                     VSLDDNALYRHPELQVLRDETQEDPRESQAAKLDLNYVSLDGNIGCMVNGAGLAMATM
                     DIIKLYGEQPANFLDVGGGATQERVSEAFRLIVSDSKVKAILVNIFGGIVRCDMIARA
                     IIHALNEARITLPVVVRLSGNNAAEGQRLLAESGLTVEAVNSLDDAAKRIIALLN"
     gene            22236..23108
                     /gene="sucD"
                     /locus_tag="J1Y83_002410"
     CDS             22236..23108
                     /gene="sucD"
                     /locus_tag="J1Y83_002410"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016655272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HBA7630554.1"
                     /translation="MSVLINKHTRVLVQGITGKNGTFHTEQAIAYGTQIVGGVTPGKG
                     GQRHLDRPVFDSMKEAMRQTEADASVIYVPAPFVLDSVVEAIEAGVKLVVVITEGVPT
                     LDMVKVRRLLDCHPDVRLIGPNCPGVITPGECKIGIMPGEIHRPGRIGIVSRSGTLTY
                     EAVAQTTALGLGQSTCIGIGGDPVPGTNFIDALRLFENDPQTDAVIMIGEIGGNAEER
                     AAEFIRHEMNKPVVGYIAGVTAPKGKRMGHAGAIISGGSGSAEDKFRAFDKAGMAWTR
                     NPALLGETLWNVIK"
BASE COUNT         5380 a         5705 c         6384 g         5678 t
ORIGIN      
        1 gctgccgaaa gtaggccgga taaggcgttt acgccgcatc cggcataaaa acagctttaa
       61 ggggcgaaag cccctctgat tatcgggttt agcgcgctat tgcctggcta ccgctgagct
      121 ccagattttg aggtgaaaac aatgaaaatg aataaaagtc tcatcgctct ctgtttatca
      181 gcagggttgc tggcaagcgc acctggaatc agccttgccg atgttaacta cgtaccgcaa
      241 aacaccagcg acgcgccagc cattccatct gccgcgctgc aacaactcac ctggacaccg
      301 gtcgatcaat ctaaaaccca gactactcaa ctgtcgaccg gcggccaaca actgaatgtc
      361 cctggcatca gtggtccggt tgctgcgtac agcgtcccgg caaacattgg tgaactgaca
      421 ctgacgctga ccagcgaagt gaacaaacaa accagcgtat ttgcgccgaa tgtgctgatt
      481 cttgatcaga acatgacccc atcagccttc ttccccagca gttatttcac ctaccaggaa
      541 cccggcgtga tgagtgcaga tcggctggaa ggcgttatgc gcctgacacc ggcgttgggg
      601 cagcaaaaac tttatgttct ggtctttacc acggaaaaag atctccagca gacgactcaa
      661 ctgctcgacc cggctaaagc ctatgccaaa ggcgtcggta actcgatccc ggatattccc
      721 gatccggttg ctcgtcatac caccgatggc ttactgaaac tgaaagtgaa aacgaactcc
      781 agctccagcg tgttggtagg accactattt ggttcttccg ctccagctcc ggttacggta
      841 ggcaacacgg cggcaccagc tgtggctgca cccgctccgg caccggtgaa gaaaagcgag
      901 ccgatgctca acgacacgga aagttatttt aataccgcga tcaaaaacgc tgtcgcgaaa
      961 ggtgatgttg ataaggcgtt aaaactgctt gatgaagctg aacgcctggg gtcgacatct
     1021 gcccgttcca cctttatcag cagtgtaaaa ggcaaggggt aattacgccc cacagtgctg
     1081 attttgcaac aactggtgcg tctcctggcg cacctttttt tctcctcctg tactattgtt
     1141 gcgtttttgt tgcgcattag ttcacttaat ttgctttaca tctcccgtaa atatttttct
     1201 gcgatacaat gcctttacgt tatgtaacgg agagttcggc atgtcacacc ccgcgttaac
     1261 gcaactgcgt gcgctgcgct attttacaga gatccctgcc ctggagccgc aactgctcga
     1321 ctggctgttg ctggaagatt ccatgacaaa acgttttgaa cagcagggaa aaacggtgag
     1381 cgtgacgatg atccgcgaag ggtttgtcga gcagaatgaa atccccgaag aactgccact
     1441 gctgccgaaa gagtctcgtt actggttacg tgaaattttg ttatgtgccg atggtgaacc
     1501 gtggcttgcc ggtcgtaccg tcgttcctgt gtcaacgtta agcgggccgg agctggcgtt
     1561 acaaaaattg ggtaaaacgc cgttaggacg ctatctgttc acatcatcga cattaacccg
     1621 ggactttatt gagataggcc gtgatgccgg actgtggggg cgacgttccc gcctgcgatt
     1681 aagcggtaaa ccgctgttgc taaccgaact gtttttaccg gcgtcaccgt tgtactaaga
     1741 ggaaaaaaat atggagtgga gtctgacgca gaataagctg ctggcgtttc atcgcttaat
     1801 gcgtacggat aagccaattg gcgcgttact gctgctctgg ccaacattat gggcgttgtg
     1861 ggtggcgaca ccgggcgttc cccagctctg gatcctggcg gtgtttgtcg cgggcgtctg
     1921 gctgatgcgc gctgctggtt gcgtggtgaa tgattatgct gaccgcaagt ttgatggtca
     1981 tgttaagcgc acggcgaacc gaccacttcc cagcggcgcg gtaacggaga aagaggcgcg
     2041 cgcgctgttt gtagtgctgg tactgatttc atttttactg gtgctgacgc tgaatacgat
     2101 gactattctg ttgtcgattg ccgcgctggc gctggcgtgg gtgtacccgt ttatgaagcg
     2161 gtatacccat ctaccacaag tggtgctggg cgcggcgttt ggctggtcga ttccaatggc
     2221 ttttgccgct gtgagtgagt cggtgccatt gagttgctgg ttaatgttcc tcgccaatat
     2281 tctctgggcg gtggcttacg acacgcagta tgcgatggtt gaccgcgatg atgatgtgaa
     2341 gattggcatt aaatccaccg caatcctgtt cggccaatac gataaattga ttattggtat
     2401 tttgcagatt ggcgttctag cgctgatggc gatcatcggt gagttaaatg gcttaggctg
     2461 gggatattac tggtcaattc tggtagctgg cgcgctgttt gtttatcaac aaaaactgat
     2521 tgccaaccgc gagcgtgaag cctgctttaa agcatttatg aataataact atgttggtct
     2581 ggtactgttt ttagggctgg caatgagtta ctggcatttc tgatgatgta aaaaagccgg
     2641 attatcatcc ggctttcttc tgggttgcct gaggcgctgc gcttctcagg cctacacaac
     2701 atatcgcaat ttattgaatt tgcagatcat ggtaggccga ataaggcgtt tgcgtcgcat
     2761 ccggcaattc tcactgatta cccttcgccc tgcgtcgcac tctcaatcgt caaacgcacg
     2821 tctgatgtaa tcaactccgc cagcaactga tataccttca tcgtttctgc cggttcggcg
     2881 tcgccgctat cgctgatata cccttcatca cgcagcgtca gcaccagaga actgaacacc
     2941 gccttgtcga agaactccgg cgcgttgata ccgtgcagca cggagagacg ttgcgcgacg
     3001 gtgcggctct ctttttccag cgtaccgcgg ttgatcgacg ggttggcact caacagccag
     3061 aaggtgatgg cataacgttg cagcgtttcg cgcgcgcctg cggccagcag ctgtagcgtg
     3121 cgagaatgcg ccgggttgat atgcaactca tcatcttgca gggtaatcag cccctgacgt
     3181 tgcatctcat ttgccagcgc atcaataacg tccggtaact cgtcgcgatc ccagcgcagg
     3241 aacagttccg ctttcagcat tgggtaaagc acattgacgt gctccatcaa tacgtcgcgg
     3301 gagatgtggc gatgctgggt gacaattgcc gccatcagcg aaggcagcac caacatatgc
     3361 gcaatgttgt tgcgatagta ggtcatcagc accgcttgct cgcgcggcag aatgatgatg
     3421 tcgccgatag tgtctttctc gacttcaaac ttgttcattt gcagcgcgtg atcgataagc
     3481 tcgctggcgc tggctgaagg aacggtagag tccgtggagt aaggcacgtt gcgcatcaga
     3541 tccaggtagc agttgagttg ctcggttaac tgctcgcggg tgagtgagcg ctgacgtgac
     3601 gccagaagcg cagtacagca caggttcatg gcgtttgccg cgcctgcgtt gttaatgcgt
     3661 accatcagat cggcagcaat attattgacc gtcggcgtta accaggccgg acgcaccgct
     3721 tcgatgggat cgatagattc acgccagtct ggtacgtgct ggttaaggta ggtcatcaac
     3781 ggcattggtt caccgaagtt gacgtaaccc tgaccgagat tacgcagctt gcttaaacca
     3841 cgcagcatct gcggcaggct ctctttctct ttcgtcgcac cgcgcagttc tttggcgtaa
     3901 gtacccactt ccatgacgtg ctcataaccg atatagatcg gaatcagcgt aatcggacga
     3961 gtgccgccac gcagcatcgc ctgaatggtc atcgatagcg taccggtttt cggatccagc
     4021 aaacgccccg tacgggaacg accgccttcc acgaaatact cgacggaata accacggctg
     4081 aacagttcgc cgagatactc acggaaaacg gtggaataaa gtttattgcc tttaaacgta
     4141 cggcgaataa agaacgcccc cagacggcgg aaaatcggcc cggccggcca gaagttcagg
     4201 ttgatcccgg cggcgatatg cggcggcacc agcccctggt gatacagcac gtaagaaagc
     4261 agcaggtagt ccatgtgact gcggtggcaa ggcacataca ctagctcatg gccgtcgtgg
     4321 gccagctggc gaacgcgctc ggcgttatgg acgttgatac cctggtaaag tcggttccag
     4381 gtgaagccca gaatgcggtc agtcaggcgg atcatctcgt aagagaaatt cgccgcaatc
     4441 tcttccatca gcgcaatcgc gttctgctgc gctttttcat gggagatttt tttgctgcgt
     4501 gcttcatctt ctaccgcttt cgcaatggcg cgggaggcga gcagcttatt aaacagatcc
     4561 tgacgagcag gcagacgtgg acccacggca gccagacgtt gacgggcaaa gtgcatacgt
     4621 gctacgcgcg ccagtttctg agcgatagtt ttatccgtgc cgtgttcatc cgccatacgg
     4681 cgcagcgaaa ctgacggcga gaaacgcaca aaactgtcgc gaccgagcca cagtacagcg
     4741 aaaaatttct gtacgccgtt aagcatacgc agcggcgggt tcacttcgcc tttttcacgc
     4801 cccggcgcgc gaccaaacat caccgataca ggcaccatct gcacatccag atttgggttg
     4861 ctgcggtgca aatcgagata gtcgtggaac agcttaatag actcttcttt cggcgtgtaa
     4921 taagtgaaca cacgcggccc gccgtgaatg aacacatagc gcggcagtag cgtgccgtcg
     4981 atttccagcg gctctaacgg gtcaggcagg tcatgtgcca gacactgggc gcgcaacgtc
     5041 agcaagtcag ctttcgagtt gtacggcaaa acgtacataa ttggacgaga ggtatccagc
     5101 cccagttccg gggcaggatc cgccggaata gacttgcttt ttaccaggat gcttaatggt
     5161 aaattcagta atttgtagta aattcgtggc cagccggaca taaacgatgt aaagcctctg
     5221 gttaataatg caaatgcgcg gcaaggatag cagaaagtca tgggaaattc tgtggtatcc
     5281 gctcatgttt cgcgcggcgt tactcaaacc cgaatcatcg gatttaacgg tacactgata
     5341 ttgacgctca taatgtaaaa aggttctttc aatggccaat aataccactg gattcatccg
     5401 aattattaaa gctgctggct attcctggaa aggtttacgc gctgcatgga tcaacgaagc
     5461 ggcattccgt caggaaggcg tagcggtatt gttagcggtg gttatcgcct gctggctgga
     5521 tgtggacgcg attacccgcg tgctgcttat cagctccgtg atgctggtga tgattgtgga
     5581 aattctcaat agcgccatcg aagctgtggt tgaccgaatt ggctctgaat accatgagct
     5641 ttccggacgc gctaaagata tgggatccgc cgcagtgctg attgcgatta tcgtcgccgt
     5701 gattacctgg tgcatcctgt tatggtcgca ttttggataa cccttccaga tttcgataaa
     5761 tctctggttt attgtgcagt ttatggttcc aaaatcgcct tttgctgtat atactcacag
     5821 cataactgta tatacaccca gggggcggaa tgaaagcgtt aacggccagg caacaagagg
     5881 tgtttgatct catccgtgat cacatcagcc agacaggtat gccgccgacg cgtgcggaaa
     5941 tcgcgcagcg tttggggttc cgttccccaa acgcggctga agaacatctg aaggcgctgg
     6001 cacgcaaagg cgttattgaa attgtttccg gcgcatcacg cgggattcgt ttattgcagg
     6061 aagaggaaga agggttgccg ctggtaggtc gtgtggctgc cggtgaaccg cttctggcgc
     6121 aacagcatat tgaaggtcat tatcaggtcg atccttcctt gttcaagccg aatgctgatt
     6181 tcctgctgcg tgtcagcggg atgtcgatga aagatatcgg cattatggat ggcgatttgc
     6241 tggcagtgca taaaactcag gatgtacgta acggtcaggt cgttgtcgca cgtattgatg
     6301 acgaagttac cgttaaacgc ctgaaaaaac agggcaataa agtcgaactg ttgccagaaa
     6361 acagcgagtt taaaccaatt gtcgttgacc ttcgtcagca gagcttcacc attgaggggc
     6421 tggcggttgg ggttattcgc aacggcgact ggctgtaaca tatctctgag accgcgatgc
     6481 cgccttgcgt cgcggtttgt ttttcatctc tcttcatcag gcttgtctgc atggcattcc
     6541 tcacttcatc tgataaagca ctctggcatc tcgccttacc catgattttc tccaatatca
     6601 ccgttccgtt gctgggactg gtcgatacgg cggtaattgg tcatcttgat agtccggttt
     6661 atttgggcgg cgtggcggtt ggtgcaacgg cgaccagctt tctctttatg ttgttgctgt
     6721 ttttacgcat gagcaccacc gggctgactg cacaggctta tggtgctaaa aatcctcagg
     6781 cattagcccg tgcgctggtg caaccgttgc tgttggcgtt gggggctggg gcgttaattg
     6841 cgctgctgcg tacgccgatt atcgatctgg cgctgcatat tgttggcggt agtgaagcag
     6901 tcctggaaca ggcgcgacgc tttcttgaaa tccgctggtt aagcgcaccg gcgtcgctgg
     6961 cgaatctggt attacttggt tggttactcg gcgtgcaata tgcccgtgcg ccagtaattt
     7021 tgttagtggt cggcaatatc ctcaacattg tgctggatgt ctggctggtg atggggctgc
     7081 atatgaacgt gcagggcgcg gctctggcaa cggttattgc ggaatatgca acattgctga
     7141 ttggtctgct aatggtgcgt aaaatcctca aactacgcgg aatctccggc gaaatgctga
     7201 aaacagcctg gcgaggaaac ttccgtcact tgctggcgct taaccgcgat atcatgctgc
     7261 gctcgctgtt gctgcaactc tgtttcggcg cgatcactgt acttggcgcg cgactgggga
     7321 gtgacattat cgctgttaac gcggttctga tgacgctact cacctttacc gcctatgcgt
     7381 tggatggttt tgcctacgcg gttgaagcac attccggtca ggcatacggt gcgcgcgacg
     7441 gtagtcaatt gctggatgtc tggcgggcag cgtgccgcca gtcggggatc gtagcgttac
     7501 tgttttcggt ggtttatttg ctggcagggg aacacatcat tgcgttgctg acgtcgttaa
     7561 cccagattca gcagctggct gaccgctatc ttatctggca ggtgattttg ccgttggtcg
     7621 gcgtctggtg ttatctgctg gacggcatgt ttataggcgc aacgcgtgcc gccgaaatgc
     7681 gtaacagtat ggcggtggcc gccgcaggtt ttgcgctgac gctccttacg ctgccgtggc
     7741 tggggaatca tggtttgtgg ctggcgttaa ccgtctttct ggcgttacgc gggctttctc
     7801 tggcggctat ctggcggcgt cactggcgta acgatacctg gtttgtcgca acgtgacggt
     7861 taaaaattct gaataaataa tcctaagcca aattgctgac tacacttaat ctcacgttca
     7921 gaagaaaagt gaacgtactc tcattcacaa cctaacgatg aggtcttgat tatgaataaa
     7981 gataaagccg gcggtaactg gaaacagttt aaaggtaaag tgaaagagca atggggcaaa
     8041 ctgaccgatg atgatatgac gatcattgaa ggaaaacgtg atcagttagt gggtaaaatc
     8101 caggaacgct acggttatca gaaagatcag gcagaaaaag aggttgatag ttgggaaaaa
     8161 cgtcatgatt accgctggta attaatccct cctgcccgac atgtacaagg atgtacaccc
     8221 ttttagcgag gtttcttttt cacctgaata gagtggtcat gatggcactg ttcaggatga
     8281 cgacacgctt ctacttctac acatgctgaa cataatccat gtgcttcaat aacattatgc
     8341 cgcagggcaa aacccatttt tgccgccagc gtatgcatga tgtcttccac gccttcagca
     8401 cattcttctt tcaccgcgcc gcagcgatcg caaataaaca tggctgaagt atgggtgggc
     8461 tgatcgaaca gatgacagag cacataactg ttggtggatt ccaccttatg cacaaaacct
     8521 tgttcaagca gaaactccag cgcgcgataa accgttggcg gcttggcttg cggttcagct
     8581 tcgcgcagca aatcaagcag atcataagcg ctgatagcgc catcctgcag actcatcagg
     8641 cgcaacactt cgagacgctg tggggtcagg cgcacattac gctgtgcgca gattttttca
     8701 gcctgcgcta ataactcctg cgttgtggtc ttttccattg ggcacctcga cagtggggtt
     8761 aagaatgtcc ttactttacc atagttctca cttaaacgcc gagaacaggg tgtcatggct
     8821 gtcacattgc ctgatcgcat attcgttatg ctatagtagc gcccccaatt taccagatgc
     8881 ttaaaaagtc gccatgccag cagaatcaca cactgtttat cccgcttacc gtttctcgat
     8941 tgccccgatg cttgactgga cggacagaca ttgccgttat ttcctgcgtc tgctttcccg
     9001 caatacgttg ctgtataccg aaatggtgac cacaggggcg attattcacg gtaaaggtga
     9061 ttacctggcg tacagtgaag aagaacatcc ggtagcgttg caactcgggg gtagcgatcc
     9121 ggcggcgctg gcacagtgtg cgaagctggc agaagcgcgt ggatatgatg agatcaacct
     9181 gaatgtcggc tgcccgtctg accgggtgca gaacggcatg tttggtgcgt gtctgatggg
     9241 taatgcgctg ctggttgccg actgcgtgaa agcgatgcgc gatgtggtgt cgattccggt
     9301 gacggtgaaa acgcgtattg gcatcgatga tcaggacagc tatgaatttc tctgcgattt
     9361 catcaacacc gtttccggca aaggtgagtg tgagatgttc atcatccacg cacgtaaagc
     9421 ctggctttct gggttaagtc cgaaagaaaa ccgtgagatc ccgcctctcg attatccgcg
     9481 tgtgtatcaa ctgaagcgtg actttccgca tctgacgatg tcgattaacg gtggtatcaa
     9541 gtcgctggaa gaagctaaag cgcatttgca acatatggat ggcgtgatgg tcgggcgcga
     9601 ggcgtatcag aatccgggta ttctggcggc ggtagaccga gagatttttg gttcctcgga
     9661 tactgatgct gatccggtgg cggtagtgcg cgccatgtat ccgtacattg agcgtgaact
     9721 cagccagggt acgtatctcg gccatattac ccggcatatg ctgggcttgt tccagggtat
     9781 tcctggcgcg cggcagtggc ggcgttattt aagtgaaaat gcccataaag cgggtgcaga
     9841 cattaatgtg ctggaacacg cgctcaaact ggtggcggat aagcgttaac ttttcaccaa
     9901 aaagtagtca aattcaccac gccctgcgca ccgtcgcggg gcgttttgct gttaaatcaa
     9961 tagattattt ttggcatgat tcttgtaatg ccagcaagag atttcatatt tgggagagca
    10021 tcatgctgga actacttttt gtaattggct tttttgtcat gctgatggtc accggtgttt
    10081 cgttgctggg tattatcgcc gcgctgattg tggcgacggc ctttatgttc ctcggcggta
    10141 tgctggcatt gatgattaag ttgctgccgt ggctgctgtt ggcggttgcg gtggtgtggg
    10201 tgatcagggc gataaaagca ccaaaagtgc cgaaatatca gcgttatgac cgctggcgtt
    10261 actaagggat tgtgcggatg atcacaacct aaggttttat ccttagaaca aaataggaat
    10321 tgataatcaa atctgtcact attgcgcgtc aaacaaattc atcgagctgt accctacata
    10381 cagccgaact ataaaagaaa gggcttccca ggtggaagcc caatttcttt tatggaatca
    10441 acaggctgga accctgcgtc gcccggcttt ccagaatctc atgcgcacgc cgcgcatcct
    10501 tcagcggata tttctgctgc tcggcgacat cgaccttaat cacaccgctg gcaatcaaag
    10561 agaacagttc attactggcc tcggttaatt cctcccgcgt ggtgatatag ccttgcaggg
    10621 aagggcgtgt cacatacaac gagcctttct gattgagaat gcctaagttc acaccggtaa
    10681 ccgcacctga tgagttgcca aagctcacca ttaagccgcg gcgttgcagg caatccagcg
    10741 accgttccca ggtgtctctg cccactgaat cgtacaccac gcgcactttc ttaccaccgg
    10801 tgatctcttt caatcgctcg accagattct cttcacgata gttaataacc tgccacgcgc
    10861 ccgcttttag cgcactctgc gctttttgcg cggttcctac ggtgccgata agtttcgcgc
    10921 ccagggcttt tgcccactgg caggcaatta agccaacgcc gccagccgct gcgtggaaca
    10981 ggaactgctc atcgggttta atttcatagg ttttgcgcag cagataataa accgttaagc
    11041 ctttcaggaa ggatgccgcc gcttgctcaa aagaaattgc cgcaggcaga atcgccgctt
    11101 tatccgcatt aatgttatgc accgagctgt aagcgcctaa cgccgactgc gcatagacta
    11161 cacgatcgcc tgccttaata tgctttacac cactgccgac tttactcacg atgcctgccg
    11221 cttcggtacc taatccgctg gggagcgatg gcggcgggta aaggccgctg cggatatacg
    11281 tgtcgataaa attgatgccg atggctttat tttcgacctg gatttcattc tccgccggat
    11341 cggcaggagt gaactctacg gcttgaagta cttccgggcc accgtgcttg tgaaattcaa
    11401 ttcgtgttgc catgtgtcct ccagaacgta tcgtcagggt ctgcttcata tggtaaagtt
    11461 tcgacccatt ctttatctcg gtaactccat tcactatggc aggaaataaa cccttcaaca
    11521 aacagcaggc tgaaccccgc gaacgcgatc cacaagttgc cgggctgaaa gtgcctccgc
    11581 actcgatcga agcggagcag tcggtgttgg gcggtttaat gctggataac gaacgctggg
    11641 atgatgtagc tgagcgtgtg gtggcagatg atttttacac ccgcccacac cgtcatatct
    11701 ttactgaaat ggcgcgtttg caggaaagcg gtagtcctat cgatctgatt acccttgcgg
    11761 aatcgctgga acgccagggg caactcgata gcgtcggtgg ttttgcttat ctggcagagc
    11821 tgtcaaaaaa tacgccaagt gcggcgaaca tcagtgcata tgcggatatc gtgcgcgaac
    11881 gtgccgttgt tcgtgagatg atctccgttg ctaacgagat tgccgaagct ggttttgatc
    11941 cacaagggcg taccagtgaa gatctgctcg accttgctga atcccgcgtc tttaaaattg
    12001 ccgaaagtcg tgcaaacaaa gacgaagggc cgaagaacat cgccgatgtg ctcgacgcaa
    12061 ccgtggcgcg tattgagcag ttgtttcagc agccacacga tggcgttacc ggggtaaata
    12121 ccggttatga cgatctcaac aaaaaaaccg ctggcttgca gccgtcggat ttgatcatcg
    12181 ttgccgcgcg tccgtcgatg ggtaaaacga catttgcgat gaacctcgtc gaaaatgcgg
    12241 cgatgttgca ggataaaccg gtgcttatct tctcgctgga gatgccatca gaacagatca
    12301 tgatgcgttc tctggcgtcg ctgtcgcgcg ttgaccagac taaaatccgt accgggcagc
    12361 tcgatgatga agactgggca cgcatttccg gcaccatggg tattttgctc gaaaaacgta
    12421 atatctatat cgatgattcc tccggcttga cgccaacgga agtgcgttcc cgcgcacgcc
    12481 gtattgcccg tgaacacggc ggcatcgggc ttatcatgat cgactacctg caactgatgc
    12541 gcgtaccggc gctttccgat aaccgtacgc tggaaattgc agaaatctcc cgctcgctga
    12601 aagcactggc gaaagaactg aacgtgccgg tggtggcgct gtcccagttg aaccgttctc
    12661 tggaacaacg tgccgacaaa cgcccggtca actccgacct gcgtgaatct ggctctatcg
    12721 agcaggatgc ggacttgatc atgtttatct atcgtgatga ggtgtatcac gaaaacagtg
    12781 atttaaaagg catcgcggaa attattatcg gtaaacaacg taacggccca atcgggacgg
    12841 tacgcctgac ctttaacggt caatggtcgc gcttcgacaa ctatgcgggg ccgcagtacg
    12901 acgacgaata ataattattt tatgaattag gtaattaaag caaacactta tcaaggaaca
    12961 caaatgcaag cggcaactgt tgtgattaac cgccgcgctc tgcgacacaa cctgcaacgt
    13021 cttcgtgaac tggcacctgc cagtaaaatg gttgcggtgg tgaaagcgaa cgcttatggt
    13081 cacggtcttc ttgagaccgc gcgaacgctc cccgatgctg acgcctttgg cgtagcccgt
    13141 ctcgaagaag ctctgcgact gcgtgcgggg ggaatcacca aacctgtact tttactcgaa
    13201 ggcttttttg atgccagaga tctaccgacg atttctgcgc aacattttca taccgccgtg
    13261 cataacgaag aacagcttgc tgcgctggaa gaggccagcc tggacgagcc ggttaccgtc
    13321 tggatgaaac tcgataccgg tatgcaccgt ctgggcgtaa ggccggaaca ggctgaggcg
    13381 ttttatcatc gcctgaccca gtgtaaaaac gttcgtcagc cggtgaatat cgtcagccat
    13441 tttgcgcgcg cggatgaacc aaaatgcggc gcaaccgaga aacaactcgc tatctttaat
    13501 accttttgcg aaggcaaacc aggtcaacgt tccattgccg catcgggtgg cattctgctg
    13561 tggccacagt cgcattttga ctgggtgcgc ccgggcatca ttctttatgg cgtctctccg
    13621 ctggaagatc gctccaccgg tgccgatttt ggctgtcagc cagtgatgtc actaacctcc
    13681 agcctgattg ccgtgcgtga gcacaaagcc ggagagcctg tcggttatgg tggaacctgg
    13741 gtaagcgaac gtgatacccg cctgggcgta gtcgcgatgg gttatggcga tggttatccg
    13801 cgcgccgcgc cgtccggtac gccagtgctg gtgaacggtc gcgaagtgcc gattgtcggg
    13861 cgagtcgcga tggatatgat ctgcgtagac ttaggtccac aggcgcagga taaagccggg
    13921 gacccggtca ttttatgggg cgaaggtttg cccgtagaac gtatcgctga aatgacgaaa
    13981 gtaagcgctt acgaacttat tacgcgcctg acttcaaggg tcgcgatgaa atacgtggat
    14041 taatcgttct gtaatatttg attgtctgtg ccggatgcgg cgtgaatgcc ttatccggcc
    14101 aataaaatcc taccaattca ataagttgat attctttcat gctcttataa aggtcgtgcc
    14161 tctggcggat gtacgtttat cattagtctc actctgttgc taattgccgt tcgcccctga
    14221 acatccactc gatcttcgcc ttcttccggt ttattgtgtt ttaaccacct gcccgtaaac
    14281 ctggagaacc atcgcgtgtt tcaaaaagtt gacgcctacg ctggcgaccc gattcttacg
    14341 cttatggagc gttttaaaga agaccctcgc tgcgataaag tgaatttaag tatcggtctg
    14401 tactacaacg aagacggaat tattccacaa ctgaaagccg tggcggatgc agcagcgcgc
    14461 ctgaatgcgc agcctcatgg cgcttcgctt tatttaccga tggaagggct taacagctat
    14521 cgccatgcca ttgcgccgct gctgtttggt gccgaccatc cggtactgca acaacagcgc
    14581 gtagcaacca ttcaaaccct tggcggctca ggggcattga aagtgggcgc agatttcctg
    14641 aaacgctact tcccggaatc aggcgtctgg gtcagcgatc ctacatggga aaaccacgta
    14701 gcaatattcg ccggggctgg attcgaagta agtacttacc cctggtatga cgaagcgact
    14761 aacggcgtgc gctttaatga cctgttgccg acgctgaaaa cattacctgc ccgcagtatt
    14821 gtgttgctgc atccatgttg ccacaaccca acgggtgctg atcttactaa tgaccaatgg
    14881 gatgcggtga ttgaaattct caaagcccgc gagcttatcc catttcttga tattgcctat
    14941 caaggatttg gtgctggtat ggaagaggat gcctacgcca ttcgcgccat tgccagcgct
    15001 ggattacccg ctctggtgag caattcgttc tcgaaaattt tctcccttta cggcgagcgt
    15061 gtcggcggac tttctgttct gtgtgaagac gccgaagccg caggccgcgt actggggcaa
    15121 ttgaaagcaa ccgttcgccg caactactcc agcccgccga attttggtgc gcaggtggtg
    15181 gctgcggtgc tgaatgacga ggcattgaaa gccagctggc tggcggaagt agaagagatg
    15241 cgtactcgca ttctggcaat gcgtcaggaa ctggtgaagg tattgagcac agagatgcca
    15301 gaacgcaatt tcgattatct gcttaatcag cgcggcatgt tcagttatac cggtttaagt
    15361 tccgctcagg ttgaccgact acgtgaagag tttggggtct atctcatcgc cagcggtcgc
    15421 atgtgtgtcg ccgggcttaa cgcgcaaaat gtgcatcgcg tggcaaaggc gtttgctgcg
    15481 gtgatgtgat tttaatcaac aggcggatct ctccgcctgt tcctgactcc agccacaccc
    15541 tactattctg tccctttttt cgcccacttt tacatcatct cttttctata agtttttgaa
    15601 aattagtgtt tttaactgtt ggtacggatt gtgcagtaat gcggctgtct atgaccatca
    15661 ggagaaaaac atggagaaca tgacctcgcc cggcacgctg cttagtggag ataacgcgac
    15721 ctggcttgaa gagtactacc agacctggtt gcgtacgccg gaaaaactcc cggaagactg
    15781 gcggcgtttt tttctttcac ctgaactgac tgtccaaagc gtatccggtg ataacaacgt
    15841 cagcggtgcg acgttaaaaa aacaggctgc ggtagtacaa ctgatcaatg cctggcgcac
    15901 gcaggggcat ctgcgcgcaa agcttgatcc acttggtttg aatcccccgg ctgacgttcc
    15961 ctccctccag cctggttttt ggggattaag cgaagaagac ttgttgcagg aatttagcgt
    16021 cacctttggc gcacacacca cgcaaatgcc acttaagcaa ctgcttaacc tgctggagca
    16081 agcctgggcg ggtagccagg cttatgaact tgctcatctg gaaaatcgtg aagaaatcaa
    16141 ctggttgtta tcacggattg agagcagcaa tgcgctacaa gctgatgtac aaacctgtat
    16201 tgctcgtttt gaaaaactga tggcggcaga gacgttagag cgttatctgc atacccgtta
    16261 tgttgggcag aaacggtttt ctctggaagg gggcgaaagt gcaatcccgg cgctcgatac
    16321 gctgacaaaa cgcctgcgtg cgcaaggtgt ggaggaaatg gtcattggca tggcgcaccg
    16381 gggaagactt aatgttctgg ttaatctgtt gaataaagac ccggcacagc tttttgctga
    16441 gtttgaaggc aagcagacga tcggcagtgg ttcaggcgat gtgaaatacc acatggggta
    16501 ttccagcaat ctggaaacac ctgccggttc gctgcatgtg gcgctggcgt ataacccctc
    16561 acatctggag atcgtgaatc cggtggtgct gggccaggtg cgagccaggc aggaacgccg
    16621 tggtgaggat ggtcaggcaa aagtggtggg cgtacttatt catggggatt ccgcgttggg
    16681 cggcctcggc gtcaaccaaa ccacgtttaa cttatcgcag actcagggct acggcactgg
    16741 cggtacgcta catctggtga ttaacaatca gattggcttc accacttccc gcttgcagga
    16801 tatgcgttct tcacggtact gcaccgatat tgccaaaatg gtagcggccc caattatcca
    16861 cgttaatggc gatgacgttg acgctgtttg ccaggtaatg gaactggcgt gcgaatggcg
    16921 agacaccttc cggcgcgaca tcatcatcga tatttgctgc ttccgtaagc acggacacaa
    16981 cgaaagcgat gagccacgtc ttacccagcc acaaatgtat caggcagtgg atgttcaccc
    17041 tggtacgctc gcccgttatg gtgaatctct tgcccgccgc ggcctgttaa ctcaggcgca
    17101 acaagatgag atgacggcac gctatcgcga ctggctggat agctgccaga aacgtgaacc
    17161 gcagcctctc aaacctgcaa ttcactcttt tagcgctaac tggtatgggt tgaccaaccc
    17221 tcactggagc gccccggtat ctacggcatt gcctcggcaa aagctggtag cgtatggtga
    17281 aatcatttct acactgccac ccgatgtggt cgcacatcct accattaaac ggcaactggc
    17341 gttacgccag gatatggccg caggtactca gccagtagac tggggaatgg ctgaaatgct
    17401 ggcctatgca tcgcttgtgg atgcgggtgt tggtgtgcgt ctttctggtg aagactcagg
    17461 gcgtggcacg ttcagtcatc gccatgcagt ggtacatcat caaactgaag cccgtcgtta
    17521 tctgccgtta cagcatatcc gcgctggtca ggcatctttc gatgtttatg attcggtgct
    17581 taatgaagaa gcgctcttag cctttgaata tggctattca acgtctgcgc ctcagcaact
    17641 ggttatttgg gaagcacagt ttggtgactt tgccaacggc gctcaggttg ctattgatca
    17701 atttatctcg tctggcgaaa ccaagtggga tcgctatagc ggtttaacca ttcttctgcc
    17761 gcatggctat gacggacaag ggccggagca ctcttctgcg cgtcctgagc gctggctgca
    17821 actgtgcgca gaaaacaaca tgcaggtggt gatgcccagt gagtcagcac aaatgttcca
    17881 tttgttgcgc ggtcaggcat tacgcccaat gcgcaaaccg ttagtgataa tgatgagtaa
    17941 gcgtttactt cgttttaaag gcgctatgag cgagctttgt gaattcactg atggcgctta
    18001 taaaccggta atcaccgatc cgcaattgca tcagtcccag aaagttaagc gcgttatttt
    18061 gtgtagcggc caggtctatt acgacgtact ggaagcccgt aagcagcgtg agcatgaaga
    18121 cgaagtggcg attgttcgcc tggagcaact ctacccgttc ccggtcgcag aactgaatga
    18181 tgttctggcg agttggccga actgctgcga atggatttgg ttgcaggaag agccagaaaa
    18241 ccagggcgcg tggcggcaga ttcgtcatga gctggcggca ctgaaaatta acactcctta
    18301 ctggcagtat gcaggccgcc cggcagcggc agctccggcg acagggtatg ggcgtgtaca
    18361 taaacaacaa atcgacgaat ttcttgctgc tgcgtttgca gatattcagc catgaaaggc
    18421 gcacaaaaat aataaggaga acattttatg atcgaaatta ctgtccctgt attaccggaa
    18481 tctgtcacgg aaggcacgct gacaacctgg tgtaaacaag aaggcgagca cgtaaaacgc
    18541 gatgatgtga ttgccgagct ggaaaccgat aaagtcattc tggaaatacc ggccccgcac
    18601 gatggcgtgt taagcaatat tatcgtcagc gaaggtagca cggtcacgtc cgcgcaactg
    18661 ttggcgcatc ttaagccgca agctgtcata gaagaaaccg tcacaccagt caccgaaacc
    18721 ctggcgatgc cttccgcacg gctggaggca cagcgtagtg gtgtcgaact tgctgacgtt
    18781 gcggggagcg ggagaaatgg acatatcctg aaagaggatg tacagcgcgt tactcctgca
    18841 ccagtcattc agcctgaacg ggtggcggag atcgcgccag ctaaaccctt gacaccgggt
    18901 gctcgtcagg aacgtcgtga gccgatgtcg cgcttacgtc agcggattgc tgagcgtctg
    18961 ctggcttccc aacaaaataa cgccattctt accacattca acgaagtaaa catgcagagc
    19021 gtgatggatt tacgcgcccg ctggaaagat cgttttgccg agaagcacgg cgtgaagctg
    19081 ggctttatgt ccttctttgt taaggccgtt acccgggcgc tggagcgctt ccctgtagtg
    19141 aatgccagcg ttgatggcaa cgagattatc tggcgtgatt attgcgatat cggcattgca
    19201 gtgagcagca accgcggcct ggtagtgcct gtgttacgca atgcgcaatc actctctttg
    19261 gtggaaattg aacggcaaat tgccgaatac gccacacagg cacgcaatgg caaattgccg
    19321 ctggaggcgt tacagggcgg cacgttctct atcaccaacg gcggtacttt tggttcgatg
    19381 atgtcaacgc caatcattaa cccgccgcag tcggccattc ttgggatgca tgccatcacg
    19441 ccgcgcccgg tcgcagaaaa cggccaggtt gttattcgtc ccatgatgta ccttgctctc
    19501 agctatgacc atcgcattat cgatggtcag gaagccgtgc agacattggt ggcaatacgt
    19561 gagctactgg aatcaccgga acaactgttg ctggatcttt aaggagggcg ccgatgagta
    19621 caatttttga tgtggcagtt atggggggtg ggccaggtgg ttacgtagcg gcactgcggg
    19681 ctgcgcagaa tgggctttcc gtagtatgta ttgatgatgg tgttaatgca cagggcgaac
    19741 cttcgccggg cggtacgtgt ctcaatgtcg gctgtattcc gtcaaaatcg ttgctgcaat
    19801 cttccgaact gtacgcgcaa gtgcagcatg aggcgagcat tcatggcgtt aatgttgacg
    19861 gcgtatcttt caatgcagct gcgatgattc aacgtaaaga cgccatagta agccgcctga
    19921 caatgggcat cggcttgctg tttaagaaaa ataaagtgaa gtacctgtgc ggcctcgcaa
    19981 cgttagaaca tgctcgggat gagatttggc aattacgtgt aaacgatcaa caaatccgcg
    20041 cccgtaatgt ggtgattgca acaggttctc agccgcgtca actgcctggc gttaccattg
    20101 ataatcagca aattctcgat aatcgcggcg ccctggcgtt aaccgctgtg ccgccgcgtc
    20161 tcggtgtgat cggcgctggc gtgattggcc ttgaactggg gtctgtgtgg aatcgggtcg
    20221 gttcagacgt cacgttgctg gagatggcgc caacgttctt gcctgcactg gaagctcgcc
    20281 tgtcgaacga agtccgtaaa gcaatgattg ccagtgggat gaaaatgcaa ctggcagtag
    20341 agattgaggc tattgaacaa cgggatgacg gtgtgcatgt gcgctggcgg cagggtgaga
    20401 agcgagaaga aagccgcttc gataaattaa ttcttgccat tggacgtgta ccgcgacttt
    20461 ctggtgtcga tctcgtgcaa ctcggtcttg aagccgataa ccgtggcggt attgccgtcg
    20521 ataacttatg ccgtaccggg aaagccgggc tgtgggcaat tggtgatgtc gtgcgagggc
    20581 cgatgctggc gcataaagcg atggctgaag gtgtggtggt tgctgatcaa attgccggac
    20641 tggcggtaga gccgattaat tttgcgctga tcccttcggt gatttacacc caaccggaag
    20701 ttgcgtgggt aggggaaaac gaggcctcac tgaaagccgc tggcagagtc tttaacaaag
    20761 gaaattctct gtttgccggt aacggtcgcg ccttagcatt aggccaggaa ggtgggcgtt
    20821 gcacgttata cagcgataaa cacactgacc gcgtacttgg cggagcgatt gttggcccgc
    20881 aggcatcgga gctgatcaat gaaattgcgc tggcgatgac ttttagcgcc tctggcgagg
    20941 atattgcctg tgcgatccat gcgcatccga cattaagcga agtcatccac gaagcagcaa
    21001 tggccttgaa taataaagca ttacacggat aacacatacc actgagggaa tatatgaatt
    21061 tacatgagta tcaggccaaa tcgttactgg ctggcatggg gatgccgtgt ccaaaagaga
    21121 tagccatcca acagatttcg caactggcag atgcctggca acatattgct tgcccgagta
    21181 aaggcgcggt acttaaagcg caggtacatg ctggtgggcg cggtaaagct ggcggcgtaa
    21241 aagtgcttaa gcagctgccg gaagcacagg cttttgtaca gcaaatgttg ggatcgcaac
    21301 tggtgactta tcagaccggg ccagaaggac agtatgtcag cagtatttta ctgtgcgaaa
    21361 acatttatcc ggtacgccag gaactctatt ttggcatggt ggtggatcgt gaaagccagc
    21421 gggtcacgtt tattgtcagc ccggaaggag gtgtggaaat tgaaaaagtc gcccatgaga
    21481 caccggagaa aatcagcagt gtcagcattg atccactgac aggcgtgcag ccttgccata
    21541 tccgcgaaat gtttgctgtt ctgcaactgg aacacggact gtttgctgcc tttagtcgcc
    21601 tggttaatca ggcgtggaaa gcctttaacg aactggattt tgccctgctg gaaattaacc
    21661 ctctggtatt gcgggaaacg ggcgaattta tgtgtgcaga cgcgaaagta tcgctggatg
    21721 ataacgcgct gtatcgtcac ccggaactcc aggtgctacg tgatgaaacc caggaagatc
    21781 cacgcgaaag ccaggcagcg aagcttgatc ttaattatgt ctcgctggac ggaaatatcg
    21841 gctgcatggt caatggtgca gggctggcta tggcaaccat ggacatcatc aagctatacg
    21901 gtgaacaacc cgcaaacttc ctggatgttg gcggtggcgc aacccaggag cgcgtcagtg
    21961 aagcctttcg gctgattgtt tccgacagca aagtgaaagc cattctggtt aatatttttg
    22021 gtgggattgt ccgttgcgac atgatcgcca gagccattat tcatgctctg aatgaggccc
    22081 gtatcaccct gcctgtcgtg gtgcgtctct ccggtaataa cgctgccgaa gggcagcggt
    22141 tactggcaga aagtggcctg acggtggaag ccgtcaattc tctggatgat gccgctaaac
    22201 gcatcattgc gttactgaat taacaggagg ttaagatgag cgttttgatc aacaaacaca
    22261 cgcgggtact ggtgcagggc attaccggca aaaacggcac atttcataca gagcaggcta
    22321 ttgcgtatgg cacgcagatt gtgggtggag tgacgccagg taagggcggg caacggcatc
    22381 tggatcgccc ggtttttgat tcaatgaaag aagcgatgcg acaaactgag gctgatgcca
    22441 gcgtgattta tgtgcctgca ccgtttgtgc tggattctgt tgttgaggct attgaagccg
    22501 gcgtcaaatt ggttgtggtg atcactgaag gtgtaccgac actggatatg gtgaaagttc
    22561 gtcggctgct tgactgccac ccggatgtgc ggctgattgg gccaaactgc ccaggagtta
    22621 tcacgccggg cgagtgcaaa atcgggatta tgcctggtga aatccaccgc ccagggcgta
    22681 ttgggattgt ttcccgctcc ggtacattaa cctacgaagc tgtagcgcaa accacggcgc
    22741 tgggacttgg gcaatccacc tgtattggta ttggcggcga tccggttccg gggactaact
    22801 ttattgatgc gctgcgttta ttcgaaaatg atccgcaaac agacgcggta attatgatcg
    22861 gtgaaattgg tggtaatgcg gaagaacggg ccgcggaatt tattcgtcat gaaatgaata
    22921 aaccggtcgt aggttatatt gccggtgtta ctgcaccaaa aggaaaacgc atggggcacg
    22981 ccggagccat tatttctggc ggtagcggca gtgcagagga taaattccgg gcgttcgata
    23041 aagcaggcat ggcatggaca agaaacccgg cgttgcttgg cgaaacgtta tggaatgtta
    23101 ttaagtaatc cataaatgaa tatcaaggga gcatatcgct ccctttt
//
LOCUS       DADSYM010000072        22069 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2415
            SAMN08039579-rid11723173.denovo.072, whole genome shotgun sequence.
ACCESSION   DADSYM010000072 DADSYM010000000
VERSION     DADSYM010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039579
            Sequence Read Archive: SRR6362533
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 22069)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22069)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980162.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 03:39:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,484
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 4,347
            CDSs (with protein)               :: 4,347
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 43 of 85
            Pseudo Genes (incomplete)         :: 37 of 85
            Pseudo Genes (internal stop)      :: 19 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22069
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039579-rid11723173.denovo.072"
                     /strain="LGE2415"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8302 N 95.9628 W"
                     /collection_date="2015-08-24"
     gene            106..1578
                     /locus_tag="J1Y83_002411"
     CDS             106..1578
                     /locus_tag="J1Y83_002411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001296416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HBA7630555.1"
                     /translation="MSGNESIAAAHYDQVTTWPRDGLQADIVHIGFGAFHRGHQAVYT
                     DLTNQLSDTRWGIFEINLFGDAQLIENLNAQNGLFSVVETSASQSTSRLVRSVAGGIH
                     TPRDGIAAAIHKLAEPQVKIVSLTITEKGYCLDPQTRSLDLTNGLINHDLQNPDAPQS
                     AIGVIVCALQQRKAAGLAAFSVLSCDNLPDNGHLTRNAVLGFARQLDQPLAQWIEENV
                     SFPGTMVDRIVPAMTESQFALLETKTGYADPCGIVCESFRQWVIEDNFVRGRPEWDKA
                     GAMFVSNVQPYEEMKLRMLNGSHSFLAYNGSLAGYEFIWQCMEDANFRSITHQLMINE
                     QARTLNPDLNINIQEYADLLIERFSNRNVAHRTGQIAMDGSQKLPQRALTPWLKLHQQ
                     KQNNAVLSLLVAGWLHYVIDAVEKSQSVADPMNDQFQALIKEQQDAWQQALALLHLSA
                     IFGDLSNHQPFINEIKIAFANIKNKGIKATISQLLSDEQK"
     gene            1575..2591
                     /locus_tag="J1Y83_002412"
     CDS             1575..2591
                     /locus_tag="J1Y83_002412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000853917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase family
                     protein"
                     /protein_id="HBA7630556.1"
                     /translation="MKTLICQQPGVMEYVEKDIPTPADNEVLLKIKAVGICGTDIHAF
                     AGRQPFFSYPRVLGHEICAEAVSRGSQCQTAQSGQRYSVIPCIPCGECAACREEKTNC
                     CERVSLYGVHQDGGFSEYLAVREDNLVPLPDEVSDSAGALVECFAIGAHAVRRAEIKA
                     EQNVLVIGAGPIGLATAAIARAKGAHVVVADIDCQRRQHVVDHLAINVFDPTQEGFIA
                     ALSEVFGGELACVVLDATGNKASMSHDVNLIRHGGKIVFIGLYIGELVINDPTFHKKE
                     TTLLSSRNATREDFALVIELMRSNKIHENLMKNQAFDFFSVGEDYQRNVVENKNMVKG
                     VITF"
     gene            2602..3612
                     /locus_tag="J1Y83_002413"
     CDS             2602..3612
                     /locus_tag="J1Y83_002413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000214530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ldh family oxidoreductase"
                     /protein_id="HBA7630557.1"
                     /translation="MTTVYVSEENLKSLVHHKLHTAGLDTDTTQQVTDVLVHADITGV
                     HSHGVMRVEHYCTRLAAGGLNKAPQFSIEQISPSVAILDSDDGMGHSALISATEHAIK
                     LAQQEGLGFVSVKNTSHCGALSYFAEMITNKGLVAIVMTQTDTCVAPHGGAERFLGTN
                     PIAFGFPVENSHPMIVDMATSATAFGKILHAKETGKHIGEGLAIDKNGYGTTDPYKIE
                     NLLPFGQHKGSGIALAIDALTGVLMNANFGNHIVRMYGDYDKMRKLASLVIAIDPKKL
                     GNPVFAKTMAKMVTELHAVKPAPGVEKVLAPNDPQMHYKEKCQQEGIPVPAGIFHYLA
                     EN"
     gene            3685..4668
                     /locus_tag="J1Y83_002414"
     CDS             3685..4668
                     /locus_tag="J1Y83_002414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000645949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HBA7630558.1"
                     /translation="MKALRPLTASLMLLTSCLLISNTTLAKTTLKLSHNQDKSHAVHK
                     ALSYLADKTKEYSNGELVICIYPNATLGNERESLELMNSGALQMVKVNAASLESFAPD
                     YSLFSLPFLFRDRDHYYRVLQSDLGKKILNSSESKGFVGITYYDGGARSFYSNKPITK
                     PEDLAGMKIRVQQSPSAIAMMKALGGVATPMAQGELYTALQQGVVDGGENNTVVYADM
                     RHAEVAKVYSRDEHTMVPDVLIISTDVLNKLGDKERTALLKAADESMMQMKDVIWPAA
                     EKEAYDKMKGMNATVVDVDKSAFKERVKPLYDEFKAKDAQSAKNLELVESM"
     gene            4710..5192
                     /locus_tag="J1Y83_002415"
     CDS             4710..5192
                     /locus_tag="J1Y83_002415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001556790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HBA7630559.1"
                     /translation="MRTLTHILNKILAGCCCIILAIMVFCVTWQVIARFIFNAPSTVL
                     DEFTQILFMWMILLGGVYTAGLKKHLAIDLLAQKLPAASVLTLDSFIQIIITVFAVIF
                     MIYGGNIVVEKAQHVGQISPVLKWPMDKVYWVMPVSGLILVWYSVMNIIDNYRKRNSH
                     "
     gene            5203..6507
                     /locus_tag="J1Y83_002416"
     CDS             5203..6507
                     /locus_tag="J1Y83_002416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HBA7630560.1"
                     /translation="MEFEYIYPVLILFGSFAVMLAIGVPITFAIGLSSLLSIITALPP
                     DAAISVISQKMTVGLDGFTLLAIPFFVLAGNIMNTGGIARRLVNLAQALVGRLPGSLA
                     HCNILANTLFGAISGSAVASAAAVGGIMSPLQEKEGYDPAFSAAVNIASAPIGLMIPP
                     SNVLIVYSLASGGTSVAALFLAGYLPGILTAVALMFVAALYARRHHYPVAERINYRQF
                     LQVFRESIPSLMLIFIIIGGIIAGVFTPTEASAIAVIYSLALAMIYREITLKKLNDIL
                     LDSVVTSSIVLLLVGCSMGMSWAMTNADVPELINELITRVSDNKWVILFIINIILLIV
                     GTFMDITPAILIFTPIFLPIAQHLGIDPIHFGIIMVFNLTIGLCTPPVGTILFVGCSI
                     GKVSIDRAIKPLLPMFLALFVVMAIICYFPQLSLMLPGLFST"
     gene            complement(6578..7990)
                     /gene="ftsP"
                     /locus_tag="J1Y83_002417"
     CDS             complement(6578..7990)
                     /gene="ftsP"
                     /locus_tag="J1Y83_002417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsP"
                     /protein_id="HBA7630561.1"
                     /translation="MSLSRRQFIQASGIALCAGAVPLKASAAGQQQPLPVPPLLESRR
                     GQPLFMTVQRAHWSFTPGTRASVWGINGRYLGPTIRVWKGDDVKLIYSNRLTENVSMT
                     VAGLQVPGPLMGGPARMMSPNADWAPVLPIRQNAATLWYHANTPNRTAQQVYNGLAGM
                     WLVEDEVSKSLPIPNHYGVDDFPVIIQDKRLDNFGTPEYNEPGSGGFVGDTLLVNGVQ
                     SPYVEVSRGWVRLRLLNASNSRRYQLQMSDGRPLHVISGDQGFLPAPVSVKQLSLAPG
                     ERREILVDMSNGDEVSITCGEAASIVDRIRGFFEPSSILVSTLVLTLRPTGLLPLVTD
                     SLPMRLLPTEIMAGSPIRSRDISLGDDPGINGQLWDVNRIDVTAQQGTWERWTVRADE
                     PQAFHIEGVMFQIRNVNGAMPFPEDRGWKDTVWVDGQVELLVYFGQPSWAHFPFYFNS
                     QTLEMADRGSIGQLLVNPVP"
     gene            complement(8065..8802)
                     /gene="plsC"
                     /locus_tag="J1Y83_002418"
                     /gene_synonym="parF"
     CDS             complement(8065..8802)
                     /gene="plsC"
                     /locus_tag="J1Y83_002418"
                     /gene_synonym="parF"
                     /EC_number="2.3.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acylglycerol-3-phosphate O-acyltransferase"
                     /protein_id="HBA7630562.1"
                     /translation="MLYIFRLIITVIYSILVCVFGSIYCLFSPRNPKHVATFGHMFGR
                     LAPLFGLKVECRKPADAESYGNAIYIANHQNNYDMVTASNIVQPPTVTVGKKSLLWIP
                     FFGQLYWLTGNLLIDRNNRTKAHGTIAEVVNHFKKRRISIWMFPEGTRSRGRGLLPFK
                     TGAFHAAIAAGVPIIPVCVSTTSNKINLNRLHNGLVIVEMLPPIDVSQYGKDQVRELA
                     AHCRSIMEQKIAELDKEVAEREAAGKV"
     gene            complement(9037..11295)
                     /gene="parC"
                     /locus_tag="J1Y83_002419"
     CDS             complement(9037..11295)
                     /gene="parC"
                     /locus_tag="J1Y83_002419"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001639862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HBA7630563.1"
                     /translation="MSDMAERLALHEFTENAYLNYSMYVIMDRALPFIGDGLKPVQRR
                     IVYAMSELGLNASAKFKKSARTVGDVLGKYHPHGDSACYEAMVLMAQPFSYRYPLVDG
                     QGNWGAPDDPKSFAAMRYTESRLSKYSELLLSELGQGTADWVPNFDGTLQEPKMLPAR
                     LPNILLNGTTGIAVGMATDIPPHNLREVAQAAIALIDQPKTTLDQLLDIVQGPDYPTE
                     AEIITSRAEIRKIYENGRGSVRMRAVWKKEDGAVVISALPHQVSGARVLEQIAAQMRN
                     KKLPMVDDLRDESDHENPTRLVIVPRSNRVDMDQVMNHLFATTDLEKSYRINLNMIGL
                     DGRPAVKNLLEILSEWLVFRRDTVRRRLNYRLEKVLKRLHILEGLLVAFLNIDEVIEI
                     IRNEDEPKPALMSRFGLTETQAEAILELKLRHLAKLEEMKIRGEQSELEKERDQLQGI
                     LASERKMNNLLKKELQADAQAYGDERRSPLQEREEAKAMSEHDMLPSEPVTIVLSQMG
                     WVRSAKGHDIDAPGLNYKAGDSFKAAVKGKSNQPVVFVDSTGRSYAIDPITLPSARGQ
                     GEPLTGKLTLPPGATVDHMLMESDDQKLLMASDAGYGFVCTFNDLVARNRAGKALITL
                     PENAHVMPPVVIEDASDMLLAITQAGRMLMFPVSDLPQLSKGKGNKIINIPSAEAARG
                     EDGLAQLYVLPPQSTLTIHVGKRKIKLRPEELQKVAGERGRRGTLMRGLQRIDRVEID
                     SPRRASSGDSEE"
     gene            complement(11433..13040)
                     /locus_tag="J1Y83_002420"
     CDS             complement(11433..13040)
                     /locus_tag="J1Y83_002420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBA7630564.1"
                     /translation="MYTRNLLWLVSLVSAAPLYAADVPANTPLAPQQVFRYNNHSDPG
                     TLDPQKVEENTAAQIVLDLFEGLVWMDGEGQVQPAQAERWEILDGGKRYIFHLRSGLQ
                     WSDGQPLTAEDFVLGWQRAVDPKTASPFAGYLAQAHINNAAAIVAGKADVTSLGVKAT
                     DDRTLEVTLEQPVPWFTTMLAWPTLFPVPHHVIAKHGDSWSKPENMVYNGAFVLDKWV
                     VNEKITARKNPKYRDAQHTVLQQVEYLALDNSVTGYNRYRAGEVDLTWVPAQQIPAIE
                     KSLPGELRIIPRLNSEYYNFNLEKPPFNDVRVRRALYLTVDRQLIAQKVLGLRTPATT
                     LTPPEVKGFSATTFDELQKPMSERVAMAKVLLKQAGYDASHPLRFELFYNKYDLHEKT
                     AIALSSEWKKWLGAQVTLRTMEWKTYLDARRAGDFMLSRQSWDATYNDASSFLNTLKS
                     DSEENVGHWKNAQYDALLNQATQITDATKRNALYQQAEVIINQQAPLIPIYYQPLIKL
                     LKPYVGGFPLHNPQDYVYSKELYIKAH"
     gene            complement(13149..13631)
                     /locus_tag="J1Y83_002421"
     CDS             complement(13149..13631)
                     /locus_tag="J1Y83_002421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HBA7630565.1"
                     /translation="MTNLTLDVNIIDFPSIPVAMLPHRCSPELLNYSVAKFIMWRKET
                     GLSPVNQSQTFGVAWDDPATTAPEAFRFDICGSVSEPIPDNRYGVSNGELTGGRYAVA
                     RHVGELDDISHTIWGIIRHWLPASGEKMRKAPILFHYTNLAEGMTERRLETDIYVPLA
                     "
     gene            complement(13684..14076)
                     /gene="ygiW"
                     /locus_tag="J1Y83_002422"
     CDS             complement(13684..14076)
                     /gene="ygiW"
                     /locus_tag="J1Y83_002422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB fold stress tolerance protein YgiW"
                     /protein_id="HBA7630566.1"
                     /translation="MKKFAAVIAVMALCSAPVMAAEQGGFSGPSATQSQAGGFQGPNG
                     SVTTVESAKSLRDDTWVTLRGNIVERISDDLYVFKDASGTINVDIDHKRWNGVTVTPK
                     DTVEIQGEVDKDWNSVEIDVKQIRKVNP"
     gene            14228..14887
                     /gene="qseB"
                     /locus_tag="J1Y83_002423"
     CDS             14228..14887
                     /gene="qseB"
                     /locus_tag="J1Y83_002423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator QseB"
                     /protein_id="HBA7630567.1"
                     /translation="MRILLIEDDMLIGDGIKTGLSKMGFSVDWFTQGRQGKEALYSAP
                     YDAVILDLTLPGMDGRDILREWREKGQREPVLILTARDALAERVEGLRLGADDYLCKP
                     FALIEVAARLEALMRRTNGQASNELRHGNVMLDPGKRIATLAGEPLTLKPKEFALLEL
                     LMRNAGRVLPRKLIEEKLYTWDEEVTSNAVEVHVHHLRRKLGSDFIRTVHGIGYTLGE
                     K"
     gene            14884..16233
                     /gene="qseC"
                     /locus_tag="J1Y83_002424"
     CDS             14884..16233
                     /gene="qseC"
                     /locus_tag="J1Y83_002424"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     QseC"
                     /protein_id="HBA7630568.1"
                     /translation="MKFTQRLSLRVRLTLIFLILASVTWLLSSFVAWKQTTDNVDELF
                     DTQLMLFAKRLSTLDLNEINAADRMAQTPNKLKHGHVDDDALTFAIFTHDGRMVLNDG
                     DNGEDIPYSYQREGFADGQLVGEDDPWRFVWMTSPDGKYRIVVGQEWEYREDMALAIV
                     AGQLIPWLVALPIMLIIMMVLLGRELAPLNKLALALRMRDPDSEKPLNATGVPSEVRP
                     LVESLNQLFARTHTMMVRERRFTSDAAHELRSPLTALKVQTEVAQLSDDDPQARKKAL
                     LQLHSGIDRATRLVDQLLTLSRLDSLDNLQDVAEIPLEDLLQSSVMDIYHTAQQAKID
                     VRLTLKANGIKRTGQPLLLSLLVRNLLDNAVRYSPQGSVVDVTLNADNFIVRDNGPGV
                     TPEALARIGERFYRPPGQTATGSGLGLSIVQRIAKLHDMNVEFGNAEQGGFEAKVSW"
     gene            16343..16924
                     /gene="mdaB"
                     /locus_tag="J1Y83_002425"
     CDS             16343..16924
                     /gene="mdaB"
                     /locus_tag="J1Y83_002425"
                     /EC_number="1.6.5.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH:quinone oxidoreductase MdaB"
                     /protein_id="HBA7630569.1"
                     /translation="MSNILIINGAKKFAHSNGQLNDTLTEVADGTLRDLGHDVRIVRA
                     DSDYDVKAEVQNFLWADVVIWQMPGWWMGAPWTVKKYIDDVFTEGHGTLYASDGRTRK
                     DPSKKYGSGGLVQGKKYMLSLTWNAPMEAFTKKDQFFHGVGVDGVYLPFHKANQFLGM
                     EPLPTFIANDVIKMPDVPRYTEEYRKHLVEIFG"
     gene            16955..17269
                     /locus_tag="J1Y83_002426"
     CDS             16955..17269
                     /locus_tag="J1Y83_002426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HBA7630570.1"
                     /translation="MITVIAEIRTRPGQHHRQAVLDQFAKIVPTVLKEEGCHGYAPMV
                     DCAAGVSFQSMAPDSIVMIEQWESIAHLEAHLQTPHMKAYSEAVKGDVLEMNIRILQP
                     GL"
     gene            complement(17314..18201)
                     /locus_tag="J1Y83_002427"
     CDS             complement(17314..18201)
                     /locus_tag="J1Y83_002427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HBA7630571.1"
                     /translation="MIQKDKARVDIFGERFRTRASQLTPGLRAVASYINEHREVVLEQ
                     TAMEIAATLNTSDATVIRAIQALGFAGLRDLKRTLEQWLGPALSSSEKMSTTVSNLTS
                     DVNTAIDFVLEGHLYTCNVLSEPENRHALAQAVALLVQARQVAIFGIGASGILADYTA
                     RLFNRIGLPATALNRTGIGLAEQLIALQRGDVLIMMAQKSAHREGLTTLREARRLGIP
                     VILLTNALDSRFSKDASIVIHVPRGDEKGKTPLHGTVLLCLEMIVWSVASAVPQRAVK
                     TIKRINDFHRGLKTGRKNG"
     gene            complement(18198..19145)
                     /locus_tag="J1Y83_002428"
     CDS             complement(18198..19145)
                     /locus_tag="J1Y83_002428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-siderophore ABC transporter
                     substrate-binding protein"
                     /protein_id="HBA7630572.1"
                     /translation="MRLFFSLLILLSFFSRATEPVQVFTDDLGRKVTVPAHPKRIVSL
                     HDLDITIPLIELGVPPVASHGRTRPDGSHFIRSGALLTGVDFDNSSIAFIGTADIDIE
                     AIVAAKPDLIITEPTRNTPIEQLEKIAPTVSIDHLKGGAPEIYRKLAELTGTQSQLAI
                     LERRYQAQINALKATLDSQKITVSVIQANQGKINVMHSYHSLGRVLRDAGFRFPPLIE
                     SIPEGGRMDVSAERLPELDADFVFATWRGDTGGKPQDELAAMEKVMPGWCQFLTACRS
                     GRYVLISREEAISNSFASLGLMAAQIQSQIAGRPLPEAK"
     gene            complement(19156..20193)
                     /locus_tag="J1Y83_002429"
     CDS             complement(19156..20193)
                     /locus_tag="J1Y83_002429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311941.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HBA7630573.1"
                     /translation="MNVGLRPLRVGKFSTLVRPKNLLLLGGLFLLAVGILIFGLMQGS
                     FSVPASEVGRALFAPENVSTDARYIVQDIRLPRVIMALLCGAMLGMAGAAMQSIARNG
                     LADPGLIGVKEGCSVAVLWLIFQFPMLGMFWRPVAGLAGGLLVALIVIFCARDISRPR
                     FVLIGIGVSWFFAAGIGVFMTTADVRDVQTALMWLSGSLHAANWMLVGISACWMLPAA
                     LLLLFTARTADIALLGHQVATGLGVNSSRLALLRVAAPIILTAVCVSCVGNIGFVGLI
                     APHISRFILRGGQTTLLLGSAVSGALLVILADSIGRLAFLPLQLPAGIIISLIGGPFF
                     LLLLWQRRNSF"
     gene            complement(20202..21185)
                     /locus_tag="J1Y83_002430"
     CDS             complement(20202..21185)
                     /locus_tag="J1Y83_002430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HBA7630574.1"
                     /translation="MMRTGLVTVIFLALLLVGCVVYPGIGARFIAPQTVLQAFLHFDP
                     QNFDHNVIVRLRLPRLAAALLTGASLGVAGALLQAVIRNPLGEPHILGLNAGAALAVV
                     AASALGLAFPVGRPLLASTGGALLFLLILLLSSAGRSGVTPMKVTLCGVALSAFVSSI
                     TAAILILDEQTLLAMRTWLAGDLAGQDWATLGTSAWFSLGGFVLALYLAPSLNMLALG
                     DRMAQGLGVSVLRTRTFTLLAIALLCGAAVSIAGPIGFVGLLVPQIVRRLVSADLRVL
                     LPLSACVGALLLLLADIIARTLFTPHELATGVMTALVGAPVFVIMATRMFK"
     gene            complement(21182..21991)
                     /locus_tag="J1Y83_002431"
     CDS             complement(21182..21991)
                     /locus_tag="J1Y83_002431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBA7630575.1"
                     /translation="MAHNAKGQGLILDNVSAGYHKKIIVDGVSFSVPTEKMTVLVGAN
                     GCGKSTLLSTIARILQPMGGSILLDGKAIHEQPTKALSRRLGILPQSPLLPEGLTVYE
                     LVSRGRFPWQNFIRQWSDADEQAVEEALKLTGTQEFAHLPVEKLSGGQRQRCWIAMVL
                     AQKTPYILLDEPTTWLDLRYQVEILELLHDLTRHHGRTVVVVLHDLNFAVNYGDTLIF
                     LRQGKVVRVLNEGEHCTPELVKAVFDVDVHASINPLTGKPFFMPFRGVEKV"
BASE COUNT         5671 a         5657 c         5378 g         5363 t
ORIGIN      
        1 tttcgtactt ttacctttct ctgcaagcgt gatgcatctc tctgtttttg gtctagtgaa
       61 ttatgtaaat tggtctacac ataagtcgtc aggagtaaga gcataatgtc agggaacgaa
      121 agtatcgctg ccgcccacta tgatcaggta accacctggc ctcgggacgg gttacaggca
      181 gatattgtcc atattggttt tggcgcgttt catcgcggac accaggctgt ctacacggat
      241 ctcactaatc aactttcgga cacccgctgg gggatctttg agatcaacct gtttggtgat
      301 gctcaactga tcgaaaactt aaatgcgcaa aatgggctgt tttcggttgt ggaaacatct
      361 gcatcgcaat ctacctcacg ccttgtgcgt tcggtggctg gcggtattca tacccccaga
      421 gatggcattg ccgcagcaat ccataaactg gctgaacctc aggtaaaaat cgtttcatta
      481 actatcaccg agaaaggcta ttgtctcgat ccgcaaacgc gatcgcttga tctcaccaat
      541 ggattaatca accacgatct acaaaatccg gatgcgcctc agtcggctat tggcgtgatc
      601 gtctgtgctt tgcaacaacg taaagcggca ggactcgccg cttttagtgt gctctcctgt
      661 gacaacttgc cagacaatgg gcatctgacg cgcaatgccg ttctcggttt tgcccgacaa
      721 ctggaccagc ccctggctca gtggatcgaa gaaaatgtct catttccagg cacgatggtc
      781 gatcgcattg ttccggcaat gactgaatcg caattcgcct tactggaaac taaaaccggt
      841 tatgccgatc cctgcgggat cgtctgcgaa tcatttcgtc agtgggtgat cgaagataat
      901 tttgtgcgcg gacgaccgga atgggataaa gccggcgcga tgtttgtcag caatgttcag
      961 ccttatgaag agatgaagtt acgcatgtta aatggtagcc attcatttct ggcttataac
     1021 ggctcactgg ctggctatga gtttatctgg caatgcatgg aagacgctaa ttttcgttcc
     1081 attacccacc aactgatgat taatgaacaa gcccgaacac ttaatccaga cttaaatatc
     1141 aacatccagg aatacgccga cctgttaatt gaacgcttta gcaaccgtaa cgttgcacac
     1201 cgtaccgggc aaatagccat ggacggttca caaaagcttc cccagcgtgc actgacgccc
     1261 tggctgaaat tgcatcagca aaaacaaaac aatgctgttc tgtcactgct cgttgctggt
     1321 tggttgcatt atgtcattga tgctgttgag aaaagccagt ctgtcgctga tccaatgaat
     1381 gaccaatttc aggcgctaat aaaggaacaa caagacgcat ggcaacaggc gctcgcatta
     1441 ctgcacctta gcgccatatt tggtgattta agcaaccatc agccatttat aaatgaaata
     1501 aaaatcgcct ttgcgaatat aaaaaacaaa ggcatcaagg ccaccatcag ccaattatta
     1561 tcggatgagc agaaatgaaa acattaattt gtcagcagcc tggcgttatg gaatatgtgg
     1621 aaaaggatat tcccacacca gcagataatg aagtgctgtt aaaaatcaaa gctgtgggta
     1681 tttgtggtac ggatattcac gcttttgccg gcagacagcc tttttttagc tacccacgtg
     1741 tattaggtca tgaaatatgc gccgaagcgg tttcgcgagg cagccagtgc caaacagcac
     1801 aatcaggcca gcgctattcc gtcatcccat gcattccgtg tggcgagtgc gcagcctgtc
     1861 gggaagagaa aacgaactgc tgcgaacgtg tttcgctgta tggcgtgcat caggatgggg
     1921 gttttagtga gtaccttgcg gtacgtgaag acaaccttgt gcctctccct gacgaggtca
     1981 gcgacagtgc cggagcattg gttgaatgtt tcgccattgg tgcacatgcc gttcgtcggg
     2041 cagagatcaa ggctgaacaa aacgtactgg tgattggcgc tgggccaatc ggtctggcta
     2101 ccgcagccat cgccagggct aaaggggcac atgttgttgt tgctgatatt gactgtcaac
     2161 gtcgccagca cgttgtggat catctggcaa ttaatgtctt cgacccaaca caggaaggtt
     2221 ttattgccgc gcttagtgaa gtgtttggcg gcgaactggc ttgcgtagta ctggatgcga
     2281 cgggaaataa agcttcaatg agtcatgatg tcaatcttat tcgtcatggc ggcaaaattg
     2341 ttttcatcgg tttgtacatt ggtgaacttg ttattaacga tccgaccttc cataaaaaag
     2401 agacaacgtt actcagcagc cgcaatgcca cacgggaaga ttttgcgttg gtgattgaac
     2461 tgatgcgcag caataaaatt cacgaaaatt taatgaaaaa ccaggcgttc gatttcttta
     2521 gtgttggcga agattaccag cgtaacgttg tagaaaataa aaatatggtc aagggtgtga
     2581 tcacttttta agggaacaaa tatgacaact gtttatgtca gtgaagaaaa tttaaaatca
     2641 ctggtgcacc acaaattaca taccgccggg ctggataccg acaccacaca acaggtgaca
     2701 gatgttttag tacatgccga tattaccggt gtgcattcac atggtgttat gcgtgtcgaa
     2761 cattattgca cccgccttgc tgccggaggg ttaaataaag ccccgcagtt tagcattgag
     2821 caaatttcac cgtcagtggc cattctcgac tctgacgacg gaatggggca ttccgcatta
     2881 attagcgcca ctgagcacgc tattaaactg gcccagcaag agggtctcgg ttttgttagc
     2941 gttaaaaaca cgtctcactg tggtgcgtta tcttattttg cagagatgat caccaacaaa
     3001 gggttggttg ctatcgtaat gacgcaaacc gatacctgcg tggctcccca tggcggcgcc
     3061 gagcgctttt tgggaactaa ccccatcgcc tttggtttcc cggtggaaaa cagccatccg
     3121 atgattgttg atatggcgac cagtgccacg gctttcggta aaatacttca tgcaaaggaa
     3181 accggaaaac atattggtga agggctggcg atagataaaa acggttatgg caccactgat
     3241 ccgtataaga ttgaaaacct gctacctttc ggccaacaca aaggttcagg cattgcactg
     3301 gctattgatg cactgactgg cgtgctgatg aatgcgaatt ttggcaacca tattgttcgc
     3361 atgtatggtg attatgacaa gatgcgtaag ctggcaagtt tggttattgc catcgatccg
     3421 aaaaagctcg gcaatcctgt ttttgcaaaa actatggcga aaatggtcac ggagctgcat
     3481 gccgttaaac cggctcctgg tgtcgaaaaa gtgttagcgc cgaacgatcc gcaaatgcac
     3541 tacaaagaaa aatgccaaca ggaaggtatt ccggttccgg caggaatatt ccattatctg
     3601 gcagagaatt aatcactatt ttttattata acgatagaaa agcgctgtat ttatcgtttc
     3661 tgttatatta accaggagca ggatatgaaa gcacttcgtc ctctgacggc atcgcttatg
     3721 ctgttaacca gttgtttatt aatatctaat accacactgg caaaaacaac attaaaactg
     3781 agtcataatc aggataaaag ccatgctgtc cataaagcat taagctattt agcggataaa
     3841 accaaagagt attctaatgg tgaattagtt atttgcattt acccaaatgc gacattaggt
     3901 aatgagcgtg aatcactgga attaatgaac tccggtgcat tacagatggt taaggttaat
     3961 gccgcatcac tagaatcatt tgctccggat tacagccttt tcagtctgcc gttcttattc
     4021 cgcgaccgtg atcactatta tcgtgttctg caaagtgatt taggtaaaaa aatacttaat
     4081 tcatcagaaa gcaaaggttt tgttggtatt acgtactatg acggcggtgc gagaagtttt
     4141 tattccaata aaccgattac aaaacctgaa gatttagcgg gaatgaaaat cagggttcaa
     4201 caaagcccca gcgccattgc aatgatgaaa gcactcggtg gtgtcgctac cccgatggcg
     4261 caaggcgaac tctacaccgc acttcagcaa ggagtggttg atggcggaga gaacaatacc
     4321 gttgtctatg ccgatatgcg tcacgccgaa gtcgcaaaag tctattcacg tgatgaacac
     4381 accatggtac ctgatgtgtt aattatcagc accgacgtgt tgaataaact tggtgataaa
     4441 gagcgcacgg cattattaaa agcagccgat gagtcaatga tgcagatgaa ggacgtcatc
     4501 tggcctgcgg ctgaaaaaga agcctacgac aaaatgaaag ggatgaacgc aacagttgtt
     4561 gatgttgata aatccgcttt caaagaacgc gtcaaaccgt tatacgatga attcaaggcg
     4621 aaagatgcac aatcagccaa aaaccttgag ctagtagaaa gtatgtaacc ctaatgggca
     4681 ggtaattcct gccttttcat ggaagactta tgcgaacact cactcatatt cttaataaga
     4741 tccttgccgg atgttgctgc attattctcg ccattatggt gttctgcgta acctggcaag
     4801 ttattgcacg ttttattttt aacgctccca gcactgtgct tgatgagttc acccaaattt
     4861 tatttatgtg gatgatttta cttggcggtg tatatactgc ggggttaaaa aaacatctgg
     4921 ccattgattt gttggctcaa aaacttcctg ctgcatctgt tctgacgctt gacagtttta
     4981 tccaaattat cattacggtc tttgcggtta ttttcatgat ctatggtggg aatattgttg
     5041 ttgaaaaagc acaacatgtt ggacaaatct ctccagtatt aaaatggcca atggataaag
     5101 tatattgggt tatgcctgtc agtgggctta ttctggtctg gtattcagta atgaatatta
     5161 ttgacaacta tcgtaaacgg aattctcatt aacgagggaa atatggaatt tgaatatatc
     5221 taccccgtct taattttatt tggcagtttt gccgtcatgc tggcaatagg tgtgccaatt
     5281 acttttgcga ttggtctttc ttcgctgtta tctattatta ctgccttgcc acccgacgct
     5341 gccatttctg ttatttcaca aaaaatgact gtagggctgg atggctttac gctattagcc
     5401 attcccttct tcgtgttagc cggaaacatt atgaataccg gtggtatagc cagacgactg
     5461 gttaacctgg cgcaagcatt agttgggcgt cttcctggct cactggctca ttgtaatatc
     5521 ctcgcgaata cactgtttgg tgcgatttca ggttcagccg ttgcgtcagc cgccgcggta
     5581 ggtgggatta tgtcaccact gcaagaaaaa gagggatatg atccggcgtt ttcagcagcg
     5641 gttaatattg cctctgcccc cattggcctg atgatcccac cgagcaatgt gttgattgtt
     5701 tattccctcg ccagtggcgg gacttctgtc gctgcgttgt tcttagctgg atatctgccg
     5761 ggcatcctca ccgctgttgc cttaatgttt gtagctgcac tttatgcccg acgtcaccat
     5821 tatccggtgg ccgaacgcat caattaccgt cagtttttac aggtattccg cgaatcaatt
     5881 cctagcctga tgcttatttt tatcattatt ggcggtatta tcgcaggggt atttacgccc
     5941 acagaagcat cagcaatagc ggtaatttat agtttagccc tggcgatgat ttaccgggaa
     6001 atcactctta agaagctcaa tgatattctg ttagattcgg tagtaaccag ttcaattgtt
     6061 ctgttactgg taggctgttc aatgggaatg tcatgggcca tgacgaatgc tgatgttcct
     6121 gagttgatca acgaactgat tacccgcgtt tcggataaca aatgggtcat tctgtttatt
     6181 atcaatatca ttctgttgat cgtcggtacc tttatggata tcacgccggc gatcctgata
     6241 tttacaccta ttttcctgcc gatcgcccag catctgggga ttgatccgat tcacttcggc
     6301 ataattatgg ttttcaactt aaccattggc ctttgcacac cacctgtcgg caccattctg
     6361 tttgtggggt gcagcattgg taaggtcagt atcgacaggg caataaaacc attactgccg
     6421 atgtttctgg cattgtttgt agtaatggca attatttgtt atttcccgca gcttagtctg
     6481 atgctgccag gattattttc gacctgatac acaatgaaga ctgcatactg agtaattttc
     6541 aatgccccac cactctgtgg ggcattgaat tggtccatta cggtaccgga ttgaccaaca
     6601 gttgcccaat cgagccacgg tccgccattt ccagcgtctg actgttgaag tagaacggga
     6661 agtgcgccca ggaaggctga ccgaaataaa caagcagctc cacttgtccg tcaacccaaa
     6721 cggtatcttt ccagcctctg tcttccggga atggcatcgc gccattcacg ttacggatct
     6781 ggaacatcac gccttcaata tggaacgctt gcggctcgtc cgcgcgcacc gtccagcgtt
     6841 cccacgttcc ttgctgcgcg gtgacatcaa tacggttgac gtcccacagt tgtccattaa
     6901 tacccgggtc atcacccaga ctaatatcgc gactgcgaat cggcgaaccg gccatgattt
     6961 cagttggcag caagcgcatc ggaagactgt cagtgaccag cggcagaagg ccggttgggc
     7021 gcagcgttag caccagcgta gaaaccagaa tgctggatgg ctcaaagaag ccacgaatac
     7081 gatcaacaat gctcgccgct tcgccacagg taatcgacac ttcatcaccg ttgctcatat
     7141 ccaccagaat ctcacgacgc tcgccaggtg ccagcgaaag ttgcttcacc gatacaggag
     7201 cgggcaggaa tccctgatcg ccagaaatca catgtaacgg gcgaccatcg ctcatctgta
     7261 gttgatagcg acgagagttc gacgcgttca acagacgcaa gcgcacccag ccacgcgaga
     7321 cttcaacgta cgggctttgt acaccgttaa ccagtagcgt atcgccaaca aagccgccgc
     7381 ttccaggttc gttatattcc ggcgtaccaa agttatccag ccgtttatcc tgaataatga
     7441 ccggaaaatc atccacacca taatggttgg ggataggcag cgatttgctg acttcatctt
     7501 ccaccagcca cattccggca aggccgttat agacctgctg agccgtgcgg ttgggagtat
     7561 tggcgtgata ccacagagta gctgcgttct ggcgaatggg cagtacgggt gcccagtcag
     7621 cgtttggcga catcatccgt gccggaccgc ccatcagcgg gccaggtacc tgtagcccgg
     7681 ccaccgtcat tgagacattt tctgtcaggc ggttgctgta aataagctta acatcgtcgc
     7741 ccttccagac gcggatagtc ggccccaggt aacgaccatt gattccccaa accgacgcgc
     7801 gtgtccctgg cgtaaatgac cagtgcgcac gttgtacagt cataaacagc ggttgcccac
     7861 ggcgagattc aagcagcggc ggaacgggta gcggttgctg ttgcccggct gcgctggcct
     7921 tcaggggaac agcgcctgca caaagtgcaa tccccgatgc ctgaatgaac tgacgccgac
     7981 tgagtgacat atttgctcca tgtaaaactg gctaaagtaa taccgtgcag gaaagtgttc
     8041 cccgcaaaaa tacagattgc cgacttaaac ttttccggcg gcttcgcgtt ctgcgacttc
     8101 tttatcgagc tcggcgattt tttgttccat tatcgaacga caatgggcag ccaactcgcg
     8161 aacctgatct ttgccatact gactgacgtc aattggcggc agcatttcga caatcaccag
     8221 accgttgtgt agccgattaa gattaatctt attcgaagtt gtagagacgc acacgggtat
     8281 aatcgggacg cccgccgcaa ttgccgcgtg aaatgccccg gtcttgaacg gcagcaggcc
     8341 gcgaccacgg ctgcgggttc cttccgggaa catccagatg gaaatacggc gttttttgaa
     8401 gtgattcacg acttccgcaa tagtgccgtg agctttagtg cgattgtttc tgtcgatcaa
     8461 taagttgccg gttaaccagt acaactgccc gaagaagggg atccacagca agctcttttt
     8521 acctaccgtc actgtcggcg gctgcacaat gttcgatgcc gtcaccatgt cataattgtt
     8581 ctggtggtta gcaatataga tagcattgcc gtagctttca gcgtctgcag gtttgcggca
     8641 ctccactttc aggccgaaaa gcggcgcaag acggccaaac atatgcccaa aggtggccac
     8701 atgtttcggg ttacgcgggc tgaaaaggca gtaaatggag ccgaatacac agactaagat
     8761 gctgtaaatc acggtaataa taagacgaaa gatatatagc ataacaccct ctgaaggtct
     8821 gagagactgg cattgtacgt cacctgaaaa tcaggttagg gcattgttaa aagccgctct
     8881 ctggaaaagc gtattgttaa tcgcaacgca tgaataaaca acggtttcaa gggaattttt
     8941 atcgtttccg ggggttgaat acgctgccgg atgacgactt aacgtttcat ccggcgtttc
     9001 ttgcaagcgg gaggaaacag cgccctcccc ggcatattac tcttcgctat caccgctgct
     9061 ggcacggcga ggagagtcga tctcaacacg atcgatacgc tgcaaaccgc gcatcaacgt
     9121 accgcggcgt ccacgttcgc cagcgacttt ctgtagctct tccggacgca gtttaatttt
     9181 gcgtttccca acatgaatgg tcagcgtgct ttgcggcggc agaacgtaca gttgcgcaag
     9241 accatcctcg ccacgcgcgg cttctgccga tggaatgttg ataatcttgt tgcctttgcc
     9301 cttcgacagc tgcggcagat cgcttaccgg gaacatcaac atacggcctg cctgagtgat
     9361 tgccagcagc atatcggaag catcttcaat caccaccggc ggcataacat gggcattttc
     9421 cggtaaggtg atcaaagctt tacctgcacg gttacgcgcc accagatcgt taaaggtgca
     9481 gacgaaaccg taacccgcat cggaagccat cagcagtttc tgatcgtcgc tttccatcag
     9541 catatggtca acggtcgctc caggcggcaa cgttaatttg ccggtgagcg gctcgccctg
     9601 accacgcgcc gacggcagcg taatcgggtc gatggcatag ctacgaccgg tggaatcaac
     9661 aaacactacc ggttggttgc tcttaccttt caccgccgct ttgaagctat cgcccgcttt
     9721 ataattcagg cccggcgcgt cgatatcatg gcctttagcg ctgcgtaccc agcccatctg
     9781 cgacagcaca atggtgacag gttcagacgg cagcatgtcg tgctcgctca tcgctttcgc
     9841 ttcttcgcgt tcctgcaacg gcgaacgacg ttcgtcaccg taggcttgcg cgtctgcctg
     9901 cagttctttc ttcagcaggt tattcatttt acgctcggaa gccaaaatgc cctgcaactg
     9961 gtcgcgctct ttttccagct cactctgctc accgcgaatc ttcatctctt ccagtttggc
    10021 aagatgacgc agtttcagtt cgaggatcgc ttccgcctgg gtttccgtaa ggccaaaccg
    10081 cgacatcagc gccggtttcg gttcatcttc attacgaatg atctcaatca cttcgtcgat
    10141 attgagaaac gccaccagca aaccttcgag gatatgcagg cgcttgagga ctttctccag
    10201 acgatagttc agtcggcggc gcacggtatc gcggcggaac accagccatt cggagaggat
    10261 ttccagcagg tttttcaccg ccggacgacc atccagaccg atcatattga ggttaatgcg
    10321 atagctcttt tccagatcgg tggtagcaaa gaggtggttc atcacctgat ccatatccac
    10381 gcggttggaa cgcggcacaa tcaccagacg ggtcgggttc tcgtggtcag attcatcgcg
    10441 cagatcgtca accatcggca gctttttgtt gcgcatttgc gcagcaattt gctccagtac
    10501 gcgcgcacct gaaacctgat gcggcaatgc gctgataacc accgcgccat cttctttctt
    10561 ccacaccgcg cgcatacgca ctgaaccacg tccgttctcg tagattttac gaatctcggc
    10621 gcgcgaagtg ataatttccg cttcagtcgg ataatccggc ccctgcacga tatccagcag
    10681 ctgatcgaga gtggttttcg gctggtcgat taatgcgatt gccgcctgag ccacttcacg
    10741 caggttatgc ggtggaatat cggtcgccat gccgacggca ataccggtgg tgccgttaag
    10801 caaaatgttt ggcagacggg caggcagcat tttcggctcc tgcaaagtgc cgtcgaagtt
    10861 tggcacccag tcagccgtcc cctgccccag ctcgctcaat agcagctcgg aatatttcga
    10921 caaccgggat tcggtgtaac gcattgccgc gaacgatttc ggatcgtccg gcgcccccca
    10981 gttcccctga ccatcaacca gcggataacg gtaagagaac ggctgcgcca tcaggaccat
    11041 cgcttcataa caggcactat cgccgtgcgg atggtattta cccagtacgt caccgacggt
    11101 acgggccgat tttttaaatt tggcgctggc attcaggccc agctcagaca tcgcatacac
    11161 aatgcggcgc tgaacaggtt tcagaccatc accaataaac ggcaacgcac ggtccatgat
    11221 cacgtacatg gagtagttta agtaggcgtt ttccgtaaat tcatgtagcg caaggcgctc
    11281 tgccatatcg ctcattaatt ctgattcctc aacttattcg ccagccttca aaggggcaat
    11341 attgccggag atactacatt atctgccgcg tcgagtcaca ggagatcagc gtgaatagcg
    11401 ccgccggacg tgctccgacg gcgataaagg catcaatgtg ccttgatata caactctttg
    11461 ctgtagacat aatcctgggg attatgcagc ggaaaaccgc caacgtaggg tttaagtagt
    11521 ttgattaacg gctgatagta gataggaatc agcggtgcct gctggttgat gatcacttct
    11581 gcctgctgat acaacgcatt acgctttgtt gcatcagtga tctgcgtggc ctggtttagt
    11641 aaggcgtcat actgcgcatt tttccagtga ccgacgtttt cttcactatc gcttttgagc
    11701 gtgttcagga agctggaggc atcattgtac gtcgcatccc acgactgccg agacagcata
    11761 aaatcaccgg ctcgtcgggc atcaagatag gttttccact ccattgtgcg cagcgtcacc
    11821 tgtgcaccca gccatttttt ccattcggaa gacaacgcta tcgcggtctt ttcatgcaga
    11881 tcgtacttgt tgtagaacag ctcaaagcgt agcggatgag aggcgtcgta tcccgcctgt
    11941 ttcagcaaga cttttgccat cgcgacacgc tcactcattg gcttttgcag ttcatcgaac
    12001 gtcgtcgcgc taaagccttt tacctctggc ggcgtcagcg tggttgcggg cgttctcaac
    12061 cccagtacct tttgcgcaat aagctgtcga tcaaccgtaa gatatagcgc ccgacgcacc
    12121 cgcacatcgt taaatggcgg tttctcaagg ttaaagttgt aatattcgct gttcagacgc
    12181 gggataattc gtagctcgcc aggcagtgat ttttcaatgg cgggaatttg ctgcgccgga
    12241 acccaggtga gatcgacctc tcccgcgcga tagcggttat agccggtgac cgaattatcc
    12301 agcgccagat actcaacctg ttgcaatact gtatgttgcg catcgcggta ctttggattt
    12361 ttgcgtgcag taatcttttc gttaactacc catttatcaa gcacaaaggc accgttgtaa
    12421 accatgttct ctggcttact ccagctatcg ccatgtttag cgatgacatg atgaggaacc
    12481 gggaacagcg tcggccaggc gagcatcgtc gtgaaccaag gaactggctg ctcaagcgta
    12541 acttcaagag tacgatcatc cgtcgctttg acacccagcg atgtaacatc tgctttaccc
    12601 gcaacaatag ccgcggcatt gttaatgtgc gcctgtgcca gatagccagc aaaagggctt
    12661 gccgttttcg ggtcaaccgc gcgctgccag ccgaggacaa aatcctctgc cgtcagaggc
    12721 tgaccgtctg accactgcaa accgctacgc agatggaaaa tatagcgctt gccgccgtca
    12781 agtatctccc agcgttcagc ctgagcgggc tgcacctggc cttcaccgtc catccatacc
    12841 agaccttcaa acagatccag cacaatctgc gcggcagtat tctcctctac cttttgcggg
    12901 tcgagcgtgc ctgggtcgct atgattgttg taacgaaaga cttgttgcgg ggcgagcggt
    12961 gtgttggcgg gaacgtcagc agcgtagaga ggagccgcac ttaccaggct gaccagccat
    13021 aataaatttc gcgtatacat ggtattcctt accggagaaa ttataagccc gacaacaata
    13081 cgcctgtggc attgtttgct caaacttatc gcgagtgatt tggctcacac tccggtaaag
    13141 attccccatc acgccaacgg cacataaata tccgtttcca gtcgccgctc tgtcatccct
    13201 tcggcaagat tggtgtagtg aaacaggatc ggggctttgc gcattttttc gccgcttgca
    13261 ggcagccagt ggcgaatgat gccccatatc gtgtgtgaaa tatcgtccag ctcgccaaca
    13321 tggcgggcca cagcataacg tccaccggta agttcaccat tacttacgcc ataacgatta
    13381 tcggggatcg gttcgctaac gctgccgcag atatcaaagc gaaacgcttc cggtgcggtg
    13441 gtggcagggt catcccaggc gacgccaaaa gtctggcttt ggttaacagg agaaagcccc
    13501 gtctctttac gccacatgat aaatttcgcc acgctgtagt tgagcaactc agggctacag
    13561 cggtgcggca acatcgccac agggattgat gggaaatcga taatatttac atccagtgtc
    13621 aggtttgtca ttttgcctcc ctgcgaaatt attcccggga acgcgtcccg ggagcggtaa
    13681 caattacgga tttactttgc ggatctgttt gacgtcaatt tcaacagagt tccagtcttt
    13741 atcgacttca ccctgaatct caaccgtatc tttcggcgtc accgtcacgc cgttccagcg
    13801 tttatggtcg atatcaacat tgatagtacc gctggcatct ttgaacacgt agagatcgtc
    13861 agagatgcgt tcaacgatat tgccgcgtag ggttacccag gtgtcgtcac gcagggattt
    13921 tgcgctttct acagtcgtta cgctgccgtt cggcccctgg aatcctccgg cctgactttg
    13981 cgttgccgat gggccagaaa aacctccctg ctctgctgcc ataaccggtg cactgcacag
    14041 ggccattact gcgattactg ctgcgaattt tttcatgttt attactccct ttaatgtctg
    14101 tttccgagca tttaacaaga tagtccttaa caacttctta agggaaaaaa ataaaattta
    14161 atgctgtaca gagcgcgtta caacacggtt tactggcagc aaatacggtt atcgcaggga
    14221 tgaaaaaatg cgaattttac tgatagaaga tgacatgctg attggcgacg gcatcaaaac
    14281 gggccttagt aaaatgggtt ttagcgtcga ctggtttaca caaggtcgtc agggaaaaga
    14341 ggcgctatat agcgcacctt atgatgcggt gatcctggat ttaaccttac caggcatgga
    14401 tggtcgcgat attttgcgcg aatggcgaga aaaaggtcag cgtgagcctg tattgatcct
    14461 gactgcgcgc gatgcgctgg cggaacgtgt agaagggctg cgtctgggag ctgacgatta
    14521 tctgtgtaaa ccttttgcgt tgatagaagt cgccgccagg ctggaagctc tgatgcgccg
    14581 aaccaacggc caggccagca acgagctgcg ccacggcaac gtcatgctcg accccggcaa
    14641 acgtatcgcc acgctggctg gcgaaccctt aacgctgaaa ccaaaagaat ttgccctgct
    14701 ggaattactg atgcgtaacg ctggtcgggt actgccgcgc aaactgattg aagagaaact
    14761 gtatacctgg gacgaagagg tcaccagtaa tgccgttgaa gtgcatgtgc atcatctgcg
    14821 acgcaaactc ggtagtgatt ttattcgtac cgttcatggt attggctaca cattaggtga
    14881 gaaatgaaat ttacccaacg tcttagtctg cgagtcaggc tgacgctaat ctttttaatt
    14941 ctggcctcgg tgacctggct gctttccagc tttgtcgcct ggaaacaaac aacggataac
    15001 gtcgatgagt tgttcgacac ccaactgatg ctgtttgcca aacggttaag tacgctcgat
    15061 ctcaacgaaa tcaacgcggc ggatcgcatg gcacagacgc caaataaatt aaaacacggt
    15121 catgttgatg acgatgcgct gacctttgcc atctttaccc acgacggcag aatggtcctt
    15181 aacgatggcg ataacggaga agatattccc tatagctatc agcgggaagg ttttgctgac
    15241 gggcaactgg tcggtgaaga cgatccctgg cgttttgtct ggatgacctc acctgatggc
    15301 aaatatcgca tcgttgtggg tcaggaatgg gaataccgtg aagacatggc gctggcgatt
    15361 gttgccggac aattgatccc gtggctggtc gcactgccga ttatgttaat catcatgatg
    15421 gtactactgg gtcgtgaact cgcgccgctg aacaaactgg cgctggcact acgtatgcgt
    15481 gacccagact cggaaaaacc actaaacgcg acgggcgtac ccagcgaagt gcgcccactg
    15541 gttgagtcgc taaatcaact gttcgcccgc acacatacga tgatggttcg tgaacgacgc
    15601 tttacctccg acgcagctca cgaacttcgt agcccgttaa cggcgctgaa agtgcaaacc
    15661 gaagttgctc agctctctga cgatgatccg caggcgcgga aaaaagcgct gctccaatta
    15721 cattccggga tcgatcgcgc tactcgtctg gttgatcaac tgctcacgct atcgcggctg
    15781 gactcactgg ataaccttca ggacgtcgcg gagatcccgc ttgaagatct cctgcaatcg
    15841 tcggtgatgg atatttacca cacggcgcag caggcgaaaa ttgacgtgcg actgacactc
    15901 aaagccaacg gcatcaaacg caccggacaa ccgctattgc taagtttgtt ggtgcgaaat
    15961 ttgctggata acgccgtgcg ctacagtcca caaggtagcg tggtagacgt cacgctgaat
    16021 gccgataatt tcatcgtgag ggataacggc cccggtgtga caccagaggc attggcgcga
    16081 attggcgaac gcttctatcg cccacccgga caaaccgcta ccggcagtgg acttgggcta
    16141 tcgattgtcc agcgaatcgc caagctgcat gacatgaatg ttgaatttgg gaatgcggaa
    16201 caaggtggat ttgaggcgaa ggtaagctgg taagggtaag acttttgcta aattcgcaaa
    16261 agactttgca cattttgcta atttcaccgt accgctctgt gacgtactat agtcggcaaa
    16321 cgtctcacct tgaggttaaa aaatgagcaa catcctgatt atcaacggcg cgaaaaaatt
    16381 cgcccactcc aatggtcaac tgaacgacac cctgaccgaa gtcgcggatg gcacactgcg
    16441 cgacctcggg catgatgtcc gcattgttcg cgccgacagc gactacgatg tcaaagcgga
    16501 agtacaaaac tttctctggg ctgatgtggt gatctggcag atgcctggct ggtggatggg
    16561 cgcgccgtgg acagtgaaaa aatatattga tgatgtattc accgaaggtc acgggactct
    16621 gtatgccagc gatggtcgta cccgcaaaga tccatcaaaa aaatacggtt ccggcggcct
    16681 ggtacagggc aaaaaatata tgctttctct gacctggaac gcaccaatgg aagccttcac
    16741 caaaaaagat cagttcttcc acggcgttgg tgttgacggt gtgtacctgc cgttccataa
    16801 agcaaaccag ttccttggca tggaaccgct gccgacattt atcgctaatg acgtgataaa
    16861 aatgcctgat gttccccgct atactgaaga atatcgcaag catcttgtgg aaatttttgg
    16921 ttaactagag ctctggcttt agaaggagtt aaccatgatt acagtaatcg cagaaatccg
    16981 tactcgtcct ggtcaacatc accgtcaggc agtgttggat cagtttgcta aaatcgttcc
    17041 aaccgtactg aaagaagaag gttgccacgg ctatgcgcca atggtggatt gcgctgctgg
    17101 cgtgagtttc cagtctatgg caccggattc tatcgtgatg attgagcagt gggaaagcat
    17161 cgcgcatctt gaagcgcatc tgcaaacccc gcacatgaaa gcatatagcg aagccgtaaa
    17221 aggtgacgtg ctggagatga atattcgtat cctgcagccg gggctttgac atttctaaaa
    17281 agaggccggt gctgctaccg gtctcagccc agattagcca ttctttcttc ccgttttcag
    17341 tcctcgatgg aaatcgttaa ttcgcttaat ggttttaacc gcacgctgcg gcactgcgga
    17401 agctaccgac cagacaatca tttccagaca caacaatacc gtaccatgta gcggcgtttt
    17461 ccctttttca tccccgcgcg gaacatgaat cacgatactg gcgtctttgc taaaacgtga
    17521 atccagcgcg tttgtcagca aaataacagg gatccccagc cgcctcgcct cacgaagcgt
    17581 tgtcagccct tcccggtgcg cagatttttg cgccatcatg atcagaacat cgccacgttg
    17641 aagcgcgatc agttgctcgg caaggccaat acccgtacgg tttagtgccg tggcaggcaa
    17701 acctatgcgg ttgaatagtc gtgcggtata gtccgccaga atacccgaag cgccaatgcc
    17761 aaaaattgcg acttgccgtg cctgcaccaa caacgctaca gcttgcgcaa gcgcgtggcg
    17821 gttttcaggc tctgacagga cattacaggt atacaaatgc ccttcaagaa caaagtcgat
    17881 tgcggtgtta acatcgctcg tcaagttact caccgtcgta gacatctttt cgctggaact
    17941 aagggctgga ccaagccact gttccagagt gcgtttcaga tcacgcagcc cggcaaaccc
    18001 cagggcctgt atagccctga taactgtggc atcggaagtg ttcaacgtgg ccgcaatttc
    18061 catcgccgtt tgctcgagta ccacttcacg atgctcatta atgtagctgg cgacagctct
    18121 taacccaggc gttaactgac tcgcgcgagt gcgaaaacgc tcaccaaaaa tatcaacccg
    18181 cgctttatct ttctggatca tttagcctcc ggtagaggtc gccctgcaat ttgtgattgt
    18241 atctgcgcag ccatcagccc cagagatgca aaggagttag aaattgcctc ttcacgagaa
    18301 atcagcacat aacgcccgga acgacaggca gtcagaaact gacaccatcc cggcatgact
    18361 ttttccattg ctgccagctc atcctgaggt ttaccaccgg tgtcaccgcg ccaggttgca
    18421 aaaacaaaat cggcatccag ttcaggtaaa cgctccgcgc tcacatccat tcgcccacct
    18481 tcaggaatgc tctcaatcag cggcggaaaa cgaaaaccag catcgcgtaa tacccgcccc
    18541 agcgaatgat agctatgcat cacattaatt tttccctggt ttgcctgaat caccgatacc
    18601 gttatttttt ggctgtccag cgtcgccttc agcgcattga tttgtgcctg atagcggcgt
    18661 tcaagtatcg ccagctgcga ctgcgttcct gtaagttctg ccagcttgcg atagatttct
    18721 ggcgcacccc ctttgagatg atcaatgctc accgtgggcg caattttttc aagttgttca
    18781 atgggcgtat tacgggtcgg ttctgtgatg atcaggtcag gctttgccgc cacaatcgct
    18841 tcaatatcaa tatcagcggt accaataaag gcgatcgatg aattatcaaa gtcgacacct
    18901 gtcaataacg cgccagatcg aatgaaatgg ctaccgtcag gccgagttcg cccgtggctg
    18961 gcgacaggag gtacaccgag ctcaatcaga ggaatagtga tatcaagatc gtgtaaagag
    19021 acaattctct ttggatgagc aggaacagtg actttgcgcc ctaaatcatc agtaaatacc
    19081 tgaacaggtt ctgttgcacg ggaaaaaaat gacaacagta tcagcagaga aaaaaataaa
    19141 cgcatataac tcccgttaaa aactgttcct gcgctgccag agcagtagca aaaaaaatgg
    19201 tccaccaatc agtgagataa tgattccggc aggcagttgc aaaggcaaaa aagccaaacg
    19261 accaatgcta tccgccagaa taactaacaa cgcccccgat accgcgctcc ccagcagtaa
    19321 tgtcgtctgc ccgccacgta gaataaaacg tgatatatgg ggggctatca atccgacaaa
    19381 accgatgtta ccaacacacg aaacgcagac ggctgtcagg attatgggtg cggcgacacg
    19441 taacaacgcc agacgagaac tattcacccc taacccggtt gcaacctgat gaccaagcag
    19501 agcgatatcc gcagtcctgg cggtgaacag caataaaaga gcggctggca acatccaaca
    19561 ggctgaaata ccgactaaca tccagttagc tgcatgcagg cttcccgata accacatcaa
    19621 tgcagtttgt acatctcgaa catctgcagt ggtcataaat acaccgatac cggcggcgaa
    19681 aaaccaggaa acaccaatac caatcagaac aaatcgcggg cgggaaatat cgcgcgcaca
    19741 aaaaatcacg attaaagcca ccaacaggcc accagcaaga ccagccactg gacgccagaa
    19801 cattcccagc attggaaact ggaaaatcag ccacagcaca gccacgctac atccttcttt
    19861 gacgccgatt aacccaggat cggcaaggcc gttacgtgca atggattgca ttgcggcccc
    19921 tgccatacct aacatggccc cgcatagcaa tgccatgatg actctcggca agcgaatatc
    19981 ctgcacaata taacgtgcgt ctgtgcttac gttttctgga gcaaacaacg ctcgtcctac
    20041 ttccgatgcc ggaacagaga aagaaccctg catcagaccg aatatcagta ttccaaccgc
    20101 aagcaggaac agcccaccaa gcaacagcag atttttaggg cgcaccagtg tcgaaaactt
    20161 accaacacgt agtgggcgta gcccgacgtt cattatccga ctcatttgaa catcctcgtc
    20221 gccataatga caaaaacagg cgcaccaact aacgccgtca tgacaccggt cgctaactca
    20281 tggggcgtga aaagcgtccg ggcaataata tctgccagta ataacaaaag cgcgccgaca
    20341 caagctgaaa gagggagcag aacgcgcaga tccgctgata ccagacgccg gacaatctgc
    20401 ggtaccaaca ggccgacaaa accaatgggg ccagcaatcg atacggctgc gccacagagc
    20461 aacgcgatcg caagcagggt aaatgttcgc gtgcggagta cagagacgcc cagcccctgc
    20521 gccatgcgat cgcccagtgc caacatgtta agggagggtg caaggtaaag ggctaacaca
    20581 aatcccccca gagaaaacca ggcggacgta cccagcgtcg cccaatcctg ccccgctaaa
    20641 tcacctgcta gccatgtccg catcgccagc agtgtttgtt cgtcaagaat caggattgca
    20701 gccgttattg aggacacgaa cgccgatagc gccacgccgc ataaagtaac tttcattggc
    20761 gttacccctg aacgccctgc ggaggaaagc agcaatatca acaggaacaa taacgcgccc
    20821 cccgtagatg ccagcagtgg gcgtccaacc ggaaaggcca gtccaagcgc actggcggca
    20881 acgacagcaa gagcggctcc ggcatttaag cctaaaatat gtggctcccc caatgggtta
    20941 cgaatgacag cctgcaacaa agcaccagca acaccaagag aagcaccggt aagcaatgct
    21001 gccgctaaac gcggcaaacg gagtcttaca attacgttgt gatcgaagtt ttgcggatca
    21061 aaatgaagaa atgcctgcaa caccgtttgt ggtgcaataa atcgggcacc aatccctgga
    21121 tataccacgc atcccaccag cagtaaggcg aggaaaatta cagtaaccag ccctgtgcgc
    21181 atcatacttt ttctacacca cgaaacggca tgaagaacgg ctttcccgtc agaggattta
    21241 ttgatgcgtg aacatcaacg tcaaagacag cttttaccag ttcaggcgtg caatgctccc
    21301 cctcattcaa cacacgcacc actttcccct ggcgtaaaaa tatcaacgta tcgccataat
    21361 tgacggcaaa attcagatca tgcaacacca cgacgactgt acgcccgtga tgacgcgtta
    21421 aatcatgtaa taactcaaga atttcaacct gataacgtaa atcaagccag gttgtgggtt
    21481 catccagcag gatataaggc gtcttttgcg ccaacaccat cgcaatccag caacgctggc
    21541 gctgcccacc agagagtttt tcaacaggta aatgtgcaaa ctcctgcgta ccggttaatt
    21601 tcagggcctc ttccaccgcc tgctcatcgg cgtcgctcca ttgacgtatg aaattttgcc
    21661 agggaaaacg cccacgcgac accagttcat aaacggttaa gccctccggc aataatggcg
    21721 actgcggcaa gatcccaagt ctacgggata acgctttcgt tggttgctcg tgaatcgctt
    21781 tgccatcaag caatatcgaa ccgcccatag gttgcaagat acgtgcaatg gtgctgagca
    21841 acgtcgactt tccacagccg tttgccccaa ccagaacggt cattttttct gtgggaacgg
    21901 agaaagaaac accatcaaca atgatcttct tgtgataccc ggcagaaaca ttgtcgagga
    21961 tcagcccctg cccttttgcg ttatgtgcca cgtgtacgcc aacttaatca aagacaaaca
    22021 aaaataaaac tcattctcct ttgagagttt gacgccacaa aatgtagta
//
LOCUS       DADSYM010000073        22050 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2415
            SAMN08039579-rid11723173.denovo.073, whole genome shotgun sequence.
ACCESSION   DADSYM010000073 DADSYM010000000
VERSION     DADSYM010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039579
            Sequence Read Archive: SRR6362533
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 22050)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22050)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980162.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 03:39:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,484
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 4,347
            CDSs (with protein)               :: 4,347
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 43 of 85
            Pseudo Genes (incomplete)         :: 37 of 85
            Pseudo Genes (internal stop)      :: 19 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22050
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039579-rid11723173.denovo.073"
                     /strain="LGE2415"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8302 N 95.9628 W"
                     /collection_date="2015-08-24"
     gene            35..310
                     /locus_tag="J1Y83_002432"
     CDS             35..310
                     /locus_tag="J1Y83_002432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HBA7630576.1"
                     /translation="MKVNLMLFSLFLFVSIMACNVFAFSISGGGSEASYKETEKTSVM
                     TTTHSTKLQPSQAILFKMREDAPPLNLTEDITPTYPTKANYLIHPLR"
     gene            complement(387..629)
                     /locus_tag="J1Y83_002433"
     CDS             complement(387..629)
                     /locus_tag="J1Y83_002433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfdY family protein"
                     /protein_id="HBA7630577.1"
                     /translation="MIYLWMFLALCIVCVSGYIGQVLNMVSAISSFFGMVILAALIYY
                     FTMWLTGGNELVTGIFMFLAPACGLMIRFMVGYGRR"
     gene            982..1902
                     /gene="lpxP"
                     /locus_tag="J1Y83_002434"
     CDS             982..1902
                     /gene="lpxP"
                     /locus_tag="J1Y83_002434"
                     /EC_number="2.3.1.242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311285.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid IV(A) palmitoleoyltransferase"
                     /protein_id="HBA7630578.1"
                     /translation="MFPKCKFSREFLHPRYWLTWFGLGVLWLWVQLPYPVLCFLGTRI
                     GAMARPFLKRRESIARKNLELCFPQHSAEEREKMIAENFRSLGMALVETGMAWFWPDS
                     RVRKWFDVEGLDNLKRAQMQNRGVMVVGVHFMSLELGGRVMGLCQPMMATYRPHNNQL
                     MEWVQTRGRMRSNKAMIGRNNLRGIVSALKKGEAVWFAPDQDYGRKGSSFAPFFAVEN
                     VATTNGTYVLSRLSGAAMLTVTMVRKADYSGYRLYITPEMEGYPADENQAAAYMNKII
                     EKEIMRAPEQYLWIHRRFKTRPVGESSLYI"
     gene            2258..2329
                     /gene="ypdK"
                     /locus_tag="J1Y83_002435"
     CDS             2258..2329
                     /gene="ypdK"
                     /locus_tag="J1Y83_002435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_077777854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein YpdK"
                     /protein_id="HBA7630579.1"
                     /translation="MKYFFMGISFMVIVWAGTFALMI"
     gene            complement(2394..3632)
                     /gene="alaC"
                     /locus_tag="J1Y83_002436"
     CDS             complement(2394..3632)
                     /gene="alaC"
                     /locus_tag="J1Y83_002436"
                     /EC_number="2.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine transaminase"
                     /protein_id="HBA7630580.1"
                     /translation="MADTRPERRFTRIDRLPPYVFNITAELKMAARRRGEDIIDFSMG
                     NPDGATPPHIVEKLCTVAQRPDTHGYSTSRGIPRLRRAISRWYQDRYDVEIDPESEAI
                     VTIGSKEGLAHLMLATLDHGDTVLVPNPSYPIHIYGAVIAGAQVRSVPLVEGVDFFNE
                     LERAIRESYPKPKMMILGFPSNPTAQCVELEFFEKVVALAKRYDVLVVHDLAYADIVY
                     DGWKAPSIMQVPGARDVAVEFFTLSKSYNMAGWRIGFMVGNKTLVSALARIKSYHDYG
                     TFTPLQVAAIAALEGDQQCVRDIAEQYKRRRDVLVKGLHEAGWMVEMPKASMYVWAKI
                     PEPYAAMGSLEFAKKLLNEAKVCVSPGIGFGDYGDTHVRFALIENRDRIRQAIRGIKA
                     MFRADGLLPASSKHIHENAE"
     gene            4009..5706
                     /locus_tag="J1Y83_002437"
     CDS             4009..5706
                     /locus_tag="J1Y83_002437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HBA7630581.1"
                     /translation="MHEIFNMLLAVFDRAALMLICLFFLIRIRLFRELLHKSAHSPKE
                     LLAVTAIFSLFALFSTWSGVPVEGSLVNVRIIAVMSGGILFGPWVGIITGVIAGIHRY
                     LIDIGGVTAIPCFITSILAGCISGWINLKIPKAQRWRVGILGGMLCETLTMILVIVWA
                     PTTALGIDIVSKIGIPMILGSVCIGFIVLLVQSVEGEKEASAARQAKLALDIANKTLP
                     LFRHVNSESLRKVCEIIRDDIHADAVAMTNTDHVLAYVGVGEHNYQNGDDFISPTTRQ
                     AMNYGKIIIKNNDEAHRTPEIHSMLVIPLWEKGVVTGTLKIYYCHAHQITSSLQEMAV
                     GLSQIISTQLEVSRAEQLREMANKAELRALQSKINPHFLFNALNAISSSIRLNPDTAR
                     QLIFNLSRYLRYNIELKDDEQIDIKKELYQIKDYIAIEQARFGDKLTVIYDIDEEVNC
                     CIPSLLIQPLVENAIVHGIQPCKGKGVVTISVAECGNRVRIAVRDTGHGIDPKVIERV
                     EANEMPGNKIGLLNVHHRVKLLYGEGLHIRRLEPGTEIAFYIPNQRSPVASQATLLL"
     gene            5721..6458
                     /locus_tag="J1Y83_002438"
     CDS             5721..6458
                     /locus_tag="J1Y83_002438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBA7630582.1"
                     /translation="MKVIIVEDEFLAQQELSWLIKEHSQMEIVGTFDDGLDVLKFLQH
                     NRVDAIFLDINIPSLDGVLLAQNISQFAHKPFIVFITAWKEHAVEAFELEAFDYILKP
                     YQESRITGMLQKLEAAWQQQQTSSTTPAATVTRENDTINLVKDERIIVTPINDIYYAE
                     AHEKMTFVYTRRESYVMPMNITEFCSKLPPSHFFRCHRSFCVNLNKIREIEPWFNNTY
                     ILRLKDLDFEVPVSRSKVKEFRQLMHL"
     gene            6471..7328
                     /locus_tag="J1Y83_002439"
     CDS             6471..7328
                     /locus_tag="J1Y83_002439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBA7630583.1"
                     /translation="MKAPGLPADQQFFADLFSGLVLNPQLLGRVWFASQPASLPVGSL
                     CIDFPRLDIVLRGEYGNLLEAKQQRMVEGEMLFIPARAANLPVNNKPVMLLSLVFAPT
                     WLGLSFYDSRTTSLLHPARQIQLPSLQRGEGEAMLTALTHLSRSPLEQNIIQPLVLSL
                     LHLCRNVVNMPPGNSQPRGDFLYHSICNWIQDNYAQPLTRESVAQFFNITPNHLSKLF
                     AQHGTMRFIEYVRWVRMAKARMILQKYHLSIHEVAQRCGFPDSDYFCRVFRRQFGLTP
                     GEYSARFQG"
     gene            complement(7331..9826)
                     /gene="ptsP"
                     /locus_tag="J1Y83_002440"
     CDS             complement(7331..9826)
                     /gene="ptsP"
                     /locus_tag="J1Y83_002440"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HBA7630584.1"
                     /translation="MLTIQFLCPLPNGLHARPAWELKEQCSQWQSEITFINHRQNAKA
                     DAKSSLALIGTGTLFNDSCSLNISGSDEEQARRVLEEYIQVRFIDSDSVQPTLAELTA
                     HPLPRSLSRLNPDLLYGNVLASGVGVGTLTLLQSDSLDSYRVIPASAQDSTLLEHSLA
                     TLAEQLNQQLRERDGESKTILSAHLSLIQDDEFAGNIRHLMAEQHQGLGAAIISNMEQ
                     ICAKLSASASDYLRERVSDIRDISEQLLHITWPELKPRNNLVLEKPTILVAEDLTPSQ
                     FLSLDLKNLAGMILEKTGRTSHTLILARASAIPVLSGLPLDAIARYAGQPAVLDAQCG
                     VLAINPNDAVSGYYQVAQTLADKRQKQQAQAAAQLAYSRDKKRIDIAANIGTALEAPG
                     AFANGAEGVGLFRTEMLYMDRDSAPDEQEQFEAYQQVLLAAGDKPIIFRTMDIGGDKS
                     IPYLNIPQEENPFLGYRAVRIYPEFAGLFRTQLRAILRAASFGNAQLMIPMVHSLDQI
                     LWVKGELQKAIVELKRDGLRHAETITLGIMVEVPSVCYIIDHFCDEVDFFSIGSNDMT
                     QYLYAVDRNNPRVSPLYNPITPSFLRMLQQIVTAAHQRGKWVGICGELGGESRYLPLL
                     LGLGLDELSMSSPRIPAVKSQLRQLDSEACRELARQAYECRSAQEIEALLTAFAPEED
                     VRPLLALENIFVDQSFSNKEQAIQFLCGNLGVNGRTEHPFELEEDVWQREEIVTTGVG
                     FGVAIPHTKSQWIRHSSISIARLVKPVDWQSEMGEVELVIMLTLGANEGMNHVKVFSQ
                     LARKLVNKNFRQSLFAAQDAQSILTLLETELTF"
     gene            complement(9851..10888)
                     /gene="ypdE"
                     /locus_tag="J1Y83_002441"
     CDS             complement(9851..10888)
                     /gene="ypdE"
                     /locus_tag="J1Y83_002441"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HBA7630585.1"
                     /translation="MDLSLLKALSEADAIASSEQEVRQILLDEADRQQKEVRFDGLGS
                     VLIRLNESTGPKVMICAHMDEVGFMVRSISREGAIDVLPVGNVRMAARQLQSVRITTR
                     EECKIPGLLDGDRQGNDVSAMRVDIGARSCDEVMQAGIRPGDRVTFDTTFQVLPHQRV
                     MGKAFDDRLGCYLLVTLLRELHDAELPAEVWLVASSSEEVGLRGGQTATRAVSPDVAI
                     VLDTACWAKNFDYGAANHRQIGNGPMLVLSDKSLIAPPKLIAWIETVAAEIGVPLQSD
                     MFSNGGTDGGAVHLTGTGVPTVVMGPATRHGHCAASIADCRDILQMQQLLSALIQRLT
                     RETVVQLTDFR"
     gene            complement(10888..11973)
                     /gene="ypdF"
                     /locus_tag="J1Y83_002442"
     CDS             complement(10888..11973)
                     /gene="ypdF"
                     /locus_tag="J1Y83_002442"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HBA7630586.1"
                     /translation="MTLLASLRDWLKAQQLDAVLLSSRQNKQPHLGISTGSGYVVISR
                     ESAHILVDSRYYADVEARTQGYQLHLLDAMHTLATIVRQIIADEQLQTLGFEGQQVSW
                     ETAHRWQFELNAKLVSATPDVLRQIKTPEEVEKIRLACGIADRGAEHIRRFIQAGMSE
                     CEIAAELEWFMRQQGAEKASFDTIVASGLRGALPHGKASDKIVAAGEFVTLDFGALYQ
                     GYCSDMTRTLLVNGEGVSAESHPLFNVYQIVLQAQLAAISVIRPGVRCQQVDDAARRV
                     ITEAGYGKFFGHNTGHAIGIEVHEEPRFSPRDTTTLQPGMLLTVEPGIYFPGQGGVRI
                     EDVVLVTPQGAEVLYAMPKTVLLTGEA"
     gene            complement(11988..13235)
                     /locus_tag="J1Y83_002443"
     CDS             complement(11988..13235)
                     /locus_tag="J1Y83_002443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HBA7630587.1"
                     /translation="MAIKKRSATVVHGASGAAAAVKNPQASKSSFWGELPQHVMSGIS
                     RMVPTLIMGGVILAFSQLIAYSWLKIPADIGIMDALNSGKFSGFDLSLLKFAWLSQSF
                     GGVLFGFAIPMFAAFVANSIGGKLAFPAGFIGGLMSTQPTQLLNFDPSIMQWATSSPV
                     PSTFIGALIISIVAGYLVKWMNQKIQLPDFLLAFKTTFLLPILSAIFVMLAMYYVITP
                     FGGWINGGIRTVLTAAGEKGALMYAMGIAAATAIDLGGPINKAAGFVAFSFTTDHVLP
                     VTARSIAIVIPPIGLGLATIIDRRLTGKRLFNAQLYPQGKTAMFLAFMGISEGAIPFA
                     LESPITAIPSYMVGAIVGSTAAVWLGAVQWFPESAIWAWPLVTNLGVYMAGIALGAII
                     TALMVVFLRLMMFRKGKLLIDSL"
     gene            complement(13257..13583)
                     /locus_tag="J1Y83_002444"
     CDS             complement(13257..13583)
                     /locus_tag="J1Y83_002444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-like phosphotransferase enzyme IIB
                     component 1"
                     /protein_id="HBA7630588.1"
                     /translation="MSKKLIALCACPMGLAHTFMAAQALEEAAVEAGYEVKIETQGAD
                     GIQNRLTAQDIAEATIIIHSVAVTPEDNERFESRDVYEITLQDAIKNAAGIIKEIEEM
                     IASEQQ"
     gene            complement(13802..14767)
                     /gene="glk"
                     /locus_tag="J1Y83_002445"
     CDS             complement(13802..14767)
                     /gene="glk"
                     /locus_tag="J1Y83_002445"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucokinase"
                     /protein_id="HBA7630589.1"
                     /translation="MTKYALVGDVGGTNARLALCDIASGEISQAKTYSGLDYPSLEAV
                     IRVYLEEHKVEVKDGCIAIACPITGDWVAMTNHTWAFSIAEMKKNLGFSHLEIINDFT
                     AVSMAIPMLKKEHLIQFGGAEPVEGKPIAVYGAGTGLGVAHLVHVDKRWVSLPGEGGH
                     VDFAPNSEEEGIILEILRAEIGHVSAERVLSGPGLVNLYRAIVKADNRLPENLKPKDI
                     TERALADSCTDCRRALSLFCVIMGRFGGNLALNLGTFGGVFIAGGIVPRFLEFFKASG
                     FRAAFEDKGRFKEYVHDIPVYLIVHDNPGLLGSGAHLRQTLGHIL"
     gene            14971..16227
                     /locus_tag="J1Y83_002446"
     CDS             14971..16227
                     /locus_tag="J1Y83_002446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ion channel protein"
                     /protein_id="HBA7630590.1"
                     /translation="MLHPRARTMLLLSLPAVAIGIASSLILIMVMKIASVLQNLLWQR
                     LPGTLGIAQDSPLWIIGVLTLTGIAVGLVIRFSQGHAGPDPACEPLIGAPIPPSALPG
                     LIVALILGLAGGVSLGPEHPIITVNIALAVAIGARLLPRVNRMEWTILASAGTIGALF
                     GTPVAAALIFSQTLNGSNEVPLWDRLFAPLMAAAAGALTTGLFFHPHFSLPIAHYGQM
                     EMTDILSGAIVAAIAIAAGMVAVWCLPRLHAMMHQMKNPVFVLGIGGFILGILGVIGG
                     PVSLFKGLDEMQQMVANQAFSTSDYFLLAVIKLAALVVAAASGFRGGRIFPAVFVGVA
                     LGLMLHEHVPAVPAAITVSCAILGIVLVVTRDGWLSLFMAAVVVPNTTLLPLLCIVML
                     PAWLLLAGKPMMMVNRPKQQPPHDNI"
     gene            16342..16668
                     /locus_tag="J1Y83_002447"
     CDS             16342..16668
                     /locus_tag="J1Y83_002447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2502 domain-containing protein"
                     /protein_id="HBA7630591.1"
                     /translation="MFRSLFLAAALMAFTPLAANAGEITLLPSIKLQIGDRDHYGNYW
                     DGGHWRDRDYWHRNYEWRKNRWWRHDNGYHRGWDKRKAYERGYREGWRDRDDHRGKGR
                     GHGHRH"
     gene            complement(16809..18047)
                     /gene="mntH"
                     /locus_tag="J1Y83_002448"
     CDS             complement(16809..18047)
                     /gene="mntH"
                     /locus_tag="J1Y83_002448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mn(2+) uptake NRAMP transporter MntH"
                     /protein_id="HBA7630592.1"
                     /translation="MTNYRVESSSGRAARKMRLALMGPAFIAAIGYIDPGNFATNIQA
                     GASFGYQLLWVVVWANLMAMLIQILSAKLGIATGKNLAEQIRDHYPRPVVWFYWVQAE
                     IIAMATDLAEFIGAAIGFKLILGVSLLQGAVLTGIATFLILMLQRRGQKPLEKVIGGL
                     LLFVAAAYIVELIFSQPNLAQLGKGMVIPSLPTSEAVFLAAGVLGATIMPHVIYLHSS
                     LTQHLHGGSRQQRYSATKWDVAIAMTIAGFVNLAMMATAAAAFHFSGHTGVADLDEAY
                     LTLQPLLSHAAATVFGLSLVAAGLSSTVVGTLAGQVVMQGFIRFHIPLWVRRTVTMLP
                     SFIVILMGLDPTRILVMSQVLLSFGIALALVPLLIFTSDSKLMGDLVNSKRVKQTGWV
                     IVVLVVALNIWLLVGTALGL"
     gene            18383..19585
                     /gene="nupC"
                     /locus_tag="J1Y83_002449"
     CDS             18383..19585
                     /gene="nupC"
                     /locus_tag="J1Y83_002449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease NupC"
                     /protein_id="HBA7630593.1"
                     /translation="MDRVLHFVLALAVVAILALLVSSDRKKIRIRYVIQLLVIEVLLA
                     WFFLNSDVGLGFVKGFSEMFEKLLGFANEGTNFVFGSMNDQGLAFFFLKVLCPIVFIS
                     ALIGILQHIRVLPVIIRAIGFLLSKVNGMGKLESFNAVSSLILGQSENFIAYKDILGK
                     ISRNRMYTMAATAMSTVSMSIVGAYMTMLEPKYVVAALVLNMFSTFIVLSLINPYRVD
                     ASEENIQMSNLHEGQSFFEMLGEYILAGFKVAIIVAAMLIGFIALIAALNALFATVTG
                     WFGYSISFQGILGYIFYPIAWVMGVPSSEALQVGSIMATKLVSNEFVAMMDLQKIAST
                     LSPRAEGIISVFLVSFANFSSIGIIAGAVKGLNEEQGNVVSRFGLKLVYGSTLVSVLS
                     ASIAALVL"
     gene            complement(19635..21824)
                     /locus_tag="J1Y83_002450"
     CDS             complement(19635..21824)
                     /locus_tag="J1Y83_002450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing phosphodiesterase"
                     /protein_id="HBA7630594.1"
                     /translation="MFVEHNLIKNIKIFTLAFTLTVVLIQLSRLISPLAVIHSNYIFL
                     AWMPLCVMLSILFIFGWRGVVPILCGMFCTNLWNLHLSFLQTAVMIGSQAFAVLCACA
                     ILRWQLGTRWRYGLTSRYVWQRLFWLGLVAPIGIKCSMYLVGNFFDFPLKISTFFGDA
                     DAIFTVVDLLSLFTAVLIYNMLFYYLARMIVSPHFAQILWRRDIAPSLSKEKRAFTLS
                     WLAALSVLLLLMCTPYENDFIAGYLVPVFFIIFTLGVGKLRYPFLNLTWAVSTLCLLN
                     YNQNFLQGVLTEYSLAFILAVLISFSVCLLYMVRIYHRSEWLNRRWHLQALTDPLTLL
                     PNFRALEQAPEQEAGKSFCCLRIDNLEFMSRHYGLMMRVHCIRSIYRTLLPLMQENEK
                     LYQLPGSELLLVLSGPETEGRLQHMVNILNSRQIHWNNTGLDMGYGAAWGRFDGNQET
                     LQPLLGQLSWLAEQSCAHHHVLALDSREEMVSGQTTKQVLLLNTIRTALDQGDLLLYA
                     QPIRNKEGEGYDEILARLKYDGGIMTPDKFLPLIAQFNLSARFDLQVLESLLKWLATH
                     PCDKKGPRFSVNLMPLTLLQKNIAGRIIRLFKRYHISPQAVILEITEEQAFSNAESSM
                     YNIEQLHKFGFRIAIDDFGTGYANYERLKRLQADIIKIDGVFVKDIVTNTLDAMIVRS
                     ITDLAKAKSLSVVAEFVETPQQQALLHKLGVQYLQGYLIGRPQPLAD"
BASE COUNT         5506 a         5840 c         5401 g         5303 t
ORIGIN      
        1 aggttttcag tgaaccattt tcaggatatt ttctatgaaa gtaaacttaa tgctttttag
       61 cttattttta tttgtctcta ttatggcatg caatgttttt gcattttcta tttctggtgg
      121 tggtagtgag gcgagctata aagagacaga aaaaacgtca gtgatgacga caacgcattc
      181 tacaaaactt cagccatcac aggcgatttt gtttaagatg agagaagatg caccaccatt
      241 aaacctcaca gaagacatca cgcctactta tccgacaaag gcgaattatc tcattcatcc
      301 tttgcgatag cgtctgtcac ctggcaggtg aacaatcatg tctgatgctg atgtgaacac
      361 ctgcccaaat gagccatcct gacccactat cgcctgccat accccaccat aaagcgaatc
      421 atcaagccac aagccggggc aagaaacata aatatccccg tcaccagttc attaccgcca
      481 gttaaccaca tggtgaagta ataaatgagt gcagcgagga tcaccatgcc gaagaaagaa
      541 gagatcgccg ataccatgtt cagtacctga ccgatgtatc cgcttacgca gacaatacac
      601 agggcgagaa acatccataa ataaatcata gctcaacctc ctgccgcgct aaaaaacctg
      661 taacttcagt cactttaata gtaagacaat ccttaacctc tggcaatttt tgctaagcat
      721 caatcaaaac agcatgttaa atgcaagact gttacgtacg gaaaaatatt tactttgcac
      781 gattaataat cagtagctga aagcagtcag cgagtgataa agtacagata ccgcattcat
      841 gctcctcgtg gttatgtcct gactagtctt tacactcttt acaggaacca ttgtcgtaca
      901 tgatggccta atcaattgag tgtaccgctg tcgacatgga atcttctgcg atacaacaat
      961 tcgtatctac agaaggtaac tatgtttcca aaatgcaaat tttcccgcga gtttctgcat
     1021 cctcgctact ggctcacgtg gtttgggctt ggtgtactct ggctttgggt acagcttcct
     1081 tatcctgttc tctgctttct cggcacgcgt attggcgcaa tggcgcgacc attcctgaaa
     1141 cggcgtgaat ctatcgcccg taaaaacctg gaactttgtt tcccgcagca ttctgcggaa
     1201 gaacgcgaga agatgattgc cgaaaacttt cgttcactcg gcatggcgct ggtagaaacc
     1261 ggcatggcgt ggttctggcc cgacagtcgc gtacgtaaat ggtttgatgt tgaagggctg
     1321 gataacctta aacgcgcaca aatgcaaaat cgcggcgtaa tggttgtcgg cgtccatttt
     1381 atgtcgctgg aactgggcgg tcgggtgatg ggactttgcc aaccaatgat ggccacctat
     1441 cgcccgcata ataatcagct gatggaatgg gtgcagaccc gtggtcgcat gcgctctaac
     1501 aaagcgatga tcggcagaaa taatctgcgc ggcattgtca gtgcactgaa gaaaggtgaa
     1561 gcggtatggt ttgctcccga tcaggattat ggtcgtaaag gcagctcctt cgcgccgttc
     1621 tttgcggtgg aaaatgtcgc cacaaccaat ggcacctatg tcctctctcg tctctctggc
     1681 gcagccatgt tgaccgtaac gatggtaaga aaagcggatt acagcggata tcgtttgtac
     1741 atcaccccag agatggaagg ctacccggca gatgaaaatc aagccgctgc ctatatgaac
     1801 aagattatcg agaaagagat catgcgcgca ccggagcagt acctctggat ccaccgtcgc
     1861 tttaaaacgc gcccggtggg tgaatcgtcg ttatacattt aacaaacggc tctcactctt
     1921 tccctcctca aatcagccat aataacctca tggtttttat ggcttttttc ttaagatttt
     1981 ttccttcgct gaatatctaa ttattaccgt cagaaattaa actgtcgccg atatgagaca
     2041 cctgtaagtt actaaaagta aataagaaaa tgcctcttgt ctcgtttcag ttttaggcgt
     2101 acattagcgc cgtctcgagc agtttagctc agaattctgg gatgatgccc acggtaaaat
     2161 acaggacgtt taaggtcgtt ttgcccggtg tcaactcact gtatcaggtg taataaagtc
     2221 attcaggcgt aacagtagtt acgcggagag atgtaaagtg aaatatttct ttatgggcat
     2281 ttcttttatg gtcatcgttt gggccggtac ttttgccctg atgatctaaa agcaaactgt
     2341 gcaatgaaaa acaggagcca tctggctcct gtttgcattt ctgacgatgg tgtttattcc
     2401 gcgttttcgt gaatatgttt gctgctggca ggtagtaaac cgtcggcacg gaacatcgct
     2461 ttaatcccac gaatcgcctg acgaatacgg tcgcggtttt caatcagtgc aaagcgcaca
     2521 tgcgtatcgc cgtagtcgcc aaagccaatc cctggagaga cacagacctt cgcttcgtta
     2581 agcaatttct tggcaaattc cagcgagccc atggcagcat acggttccgg gattttcgcc
     2641 cagacataca tcgaagcctt cggcatttcg accatccagc ccgcttcatg cagccctttc
     2701 accagcacat cacggcggcg tttgtactgt tcggcaatat cgcgcacgca ctgttgatcg
     2761 ccctccagcg ccgcaatagc cgccacctgc aacggcgtaa aggtgccgta atcgtgatag
     2821 cttttaatac gtgccaacgc gctgaccagc gttttgttac cgaccataaa gccgatacgc
     2881 cagcccgcca tgttgtagct tttcgacagc gtaaagaact cgaccgccac gtcgcgtgca
     2941 cctggcacct gcatgattga cggcgctttc cagccatcgt agacgatatc ggcataggcc
     3001 aggtcgtgga ccaccagcac atcgtagcgt ttcgccagcg ccacaacctt ctcaaagaat
     3061 tccagttcca cgcattgcgc ggttgggttt gacgggaagc cgaggatcat catcttcggt
     3121 ttcggataac tttcacgaat ggcgcgctcc agttcgttga agaaatcgac accttccacc
     3181 agcggcactg agcgtacctg cgccccggca atcaccgcgc cgtaaatatg aatggggtag
     3241 cttggattcg gcaccagcac tgtgtcacca tgatccagcg tcgccagcat caggtgcgcc
     3301 agtccctctt tcgaaccaat agtgacgatg gcttctgatt ccgggtcgat ttcaacgtcg
     3361 tagcgatcct gataccagcg ggaaatggcg cgacgtaacc gcggaatgcc gcgtgaagtg
     3421 gagtaaccat gcgtgtccgg gcgctgggcc accgtacata atttttcgac gatatgcggc
     3481 ggagtcgcac cgtccgggtt acccatactg aaatcgataa tatcttcgcc gcgccgacgc
     3541 gcagccattt tcagttcagc ggtaatgtta aaaacgtagg gcgggagacg atcaatgcgc
     3601 gtaaagcgac gttcagggcg agtgtcagcc ataatttcct cagattaacg ttagcgcccg
     3661 gaccgtccga gcgacgctgc cacgatggtg gctgttttga aaatagccag attaatttct
     3721 gtctgtccag cgttttagaa catttttatc aatggcttta aatgaggaac tccacttcaa
     3781 aaacaggaaa tttatctacg ctaacaagaa aattaccact cattttatta acattataat
     3841 ttcaataaat ttaccaacca gatgtgcatt atcaagagat gcattgctaa tacaccattt
     3901 atctttcaat ccccttttaa aattgtggtt tttcctcaaa aacggcctgc tggtcatcag
     3961 ccttcaggtt acctatcata gaggtttaat ccttattcag agtcacccgt gcacgaaata
     4021 ttcaacatgc tgctggcggt cttcgatcgg gcagcgttaa tgcttatctg cctgtttttt
     4081 ctcatccgta tccgcctgtt tcgcgaactg ttgcacaagt cggcacactc accgaaagag
     4141 ctgctcgccg ttaccgccat tttctcgctg ttcgccctgt tcagcacctg gtccggcgtt
     4201 cccgtagaag gctcgctggt gaacgtacgt attatcgcgg tgatgtcagg cgggattctg
     4261 tttggcccgt gggtgggcat cattaccggc gtgattgcgg gtattcaccg ttatttaatt
     4321 gacatcggcg gcgtgacggc gatcccctgt tttatcacca gcattctggc gggttgtata
     4381 tcgggctgga tcaacctgaa aatccccaaa gcacagcgct ggcgcgtcgg tattctcggc
     4441 ggcatgttgt gtgaaacgtt gaccatgatt ctggtgattg tctgggcacc cactaccgca
     4501 ttggggatcg atatcgtctc taaaatcggg attccaatga tcctcggcag cgtctgtatc
     4561 ggctttattg tgttgctggt acaaagtgtt gagggagaaa aagaggccag cgccgcgcgg
     4621 caggccaaac tggcgctgga tatcgccaac aaaacgctgc cgctgtttcg ccatgtcaat
     4681 agcgagtcat tacgcaaagt ctgcgaaatt atccgcgatg acattcacgc cgatgcggtg
     4741 gcgatgacta ataccgatca tgtgctggcc tacgttggcg tgggcgaaca caactatcag
     4801 aatggcgatg acttcattag cccgactacc cgccaggcga tgaattacgg gaaaatcatt
     4861 attaaaaaca atgatgaagc ccaccgcaca ccagagattc attccatgct ggtgatccca
     4921 ttgtgggaga aaggcgttgt gaccggaacg ctgaaaattt actactgcca cgcgcatcag
     4981 ataacctcgt cattacagga aatggcggtc ggtctgtcac aaatcatctc cacgcaactg
     5041 gaggtttcgc gcgctgagca gctgcgtgaa atggcaaata aggcagagct tcgcgccctg
     5101 caaagcaaaa ttaatcccca tttcctgttt aacgctttga acgctatttc atcgtcaatc
     5161 cgtctgaatc cggatactgc tcgccagctg atctttaatt tgtcgcgcta tctgcgctat
     5221 aacattgaat taaaagacga tgagcaaatc gatatcaaaa aagagctgta tcaaattaaa
     5281 gactatattg ccattgagca ggcccgcttt ggcgacaagc tgacggttat ctatgatatt
     5341 gatgaagagg tgaattgctg cattcccagc ctgctgatcc agccgttggt ggaaaacgcc
     5401 attgtccacg gtatccagcc ttgcaaaggt aaaggcgttg tcaccatcag cgttgcagag
     5461 tgcggtaatc gggtacgcat tgcggtgcga gataccgggc acggcatcga tccaaaggtg
     5521 attgagcggg tcgaagcgaa cgaaatgccg ggcaataaaa ttggcctgct gaatgtccat
     5581 catcgcgtga agttattgta tggcgagggg ctgcatatcc gccgcctgga accaggaacg
     5641 gaaattgcat tttacattcc taaccaacgc agcccagtcg cctcacaggc taccttattg
     5701 ctttgagccg gagtgatatt gtgaaagtca tcattgttga agacgaattc ctggcacaac
     5761 aggaactgag ctggctaatt aaagaacaca gccagatgga gattgtcggc acctttgacg
     5821 acggtctgga cgtgttgaaa tttttgcagc ataaccgcgt cgacgccatt tttctggata
     5881 tcaatattcc gtcgctggac ggtgtgttgc tggcgcaaaa catcagccag ttcgcccata
     5941 aaccgtttat tgtgttcatc accgcgtgga aagaacatgc ggtggaagca tttgaactgg
     6001 aagcgtttga ctacattctc aaaccgtatc aggaatcacg cattaccggg atgctgcaaa
     6061 aactggaagc agcctggcaa caacagcaga ccagcagtac gacgcctgcc gcgacggtaa
     6121 cgcgtgagaa tgacaccatt aatctggtga aagatgagcg aatcatcgtc acgccaatta
     6181 acgatatcta ttacgccgaa gcgcacgaga aaatgacctt tgtctatacg cggcgtgaat
     6241 cctacgtaat gccgatgaac attaccgagt tttgcagcaa gctgccgccg tcgcattttt
     6301 tccgctgcca tcgctcattt tgtgtcaatc tgaacaaaat acgcgaaatc gaaccgtggt
     6361 ttaataacac ctacattctg cggctgaaag atctggattt tgaagtgccg gtcagccgta
     6421 gcaaagttaa agaattccgc cagttaatgc atctgtaatc gggaactttc atgaaagcac
     6481 caggcttgcc cgccgatcaa caatttttcg ccgatctgtt cagcggcctg gtgcttaacc
     6541 cgcaactcct tgggcgcgtc tggtttgcca gccagcccgc ctcattgcca gtgggcagtt
     6601 tatgtattga ttttccccgt ctggatatcg tgctacgcgg cgaatacggc aatctgctgg
     6661 aagcaaagca gcaacgtatg gtggaaggag aaatgctgtt tattccagca cgcgcggcta
     6721 atttaccggt caacaacaaa ccggtgatgc tgttaagcct ggtgttcgca ccgacctggc
     6781 ttgggttatc gttttacgat agccgcacca cgtcgttgtt acatcctgct cgccagatcc
     6841 agcttcccag cctgcaacgc ggtgaaggtg aagcgatgct taccgccctc acccatctta
     6901 gccgttcgcc actggagcag aatatcattc agccgctggt gttaagtttg ctgcatcttt
     6961 gccgtaacgt ggtgaatatg ccgccgggca attcgcaacc gcgcggcgat tttctctatc
     7021 acagcatttg taactggatt caggataatt atgcccagcc gctcacccgc gagagcgtgg
     7081 cgcagttttt taatatcacg cccaatcatc tgtcaaaact gtttgctcag catggaacaa
     7141 tgcgttttat cgagtatgtg cgttgggtac gaatggcaaa agcgaggatg attttgcaaa
     7201 aatatcatct gtcgattcat gaagtggcgc agcgttgcgg gtttccggat agcgactatt
     7261 tttgtcgcgt tttccggcgt cagtttggtc tgacgccggg agagtatagc gcacgttttc
     7321 agggctaacg tcagaaggtt aattccgttt ccagcagcgt cagaatactt tgtgcatctt
     7381 gcgcggcaaa cagcgactgg cggaagtttt tattcaccag tttacgcgcc agctgcgaga
     7441 agactttcac atgattcatc ccttcgttag cgcccagcgt cagcatgatc accagttcga
     7501 cttcgcccat ttctgactgc cagtcaaccg gtttcaccaa ccgggcaatg ctgatactgg
     7561 aatgacggat ccactgtgat ttggtgtgcg ggatcgccac gccaaaacca acgccagtag
     7621 taacaatctc ttcccgctgc cagacatctt cttccagctc gaacggatgt tcagtacgcc
     7681 cgttaacacc gaggttaccg cacaggaact ggatcgcctg ctctttatta ctaaaggact
     7741 gatcaacaaa gatattctct agtgccagca gtgggcgaac gtcttcttcc ggcgcaaacg
     7801 cggtaagtaa cgcttcaatt tcctgcgcgc tgcggcactc gtacgcctga cgcgccagtt
     7861 cccgacacgc ctcgctatcc agttgacgaa gctggctttt cactgccgga atacgcgggc
     7921 tactcatgct tagctcatcc aggcccagcc ctagcagtag cggcagataa cgactttcac
     7981 cgcccagttc accgcaaata cctacccatt tgccgcgctg atgcgcagcg gtaacaatct
     8041 gctgcaacat gcgcaggaac gatggcgtaa tcgggttata cagcggcgat acgcgcgggt
     8101 tattacgatc gactgcatac agatactggg tcatatcgtt agaaccgata ctgaagaaat
     8161 cgacctcatc gcagaagtgg tcgatgatgt agcacaccga cggcacttcc accatgatac
     8221 ccagcgtaat cgtctctgca tgacgcaggc catcgcgctt aagctcaacg atcgcttttt
     8281 ggagctcacc cttcacccat aagatctgat cgagactgtg gaccattggg atcatcaact
     8341 gggcgttgcc gaaactggca gcgcgcaaaa tcgcccgcaa ttgagtacgg aacaggccag
     8401 caaattccgg gtaaatacgt accgcgcgat agccgaggaa cgggttctct tcctggggaa
     8461 tgttgaggta aggaatgctt ttatcgccac cgatgtccat cgtgcggaag ataatcggct
     8521 tgtcgcccgc cgccagtagc acctgctggt acgcttcaaa ctgttcctgc tcgtccggcg
     8581 cgctgtcgcg atccatatag agcatttcgg tacgaaacag cccaacgcct tccgctccgt
     8641 tggcaaacgc gcctggggct tccagagcgg tgccaatatt cgccgcaata tcaatacgct
     8701 ttttatcacg ggaataggcc agctgcgcgg cagcttgcgc ctgttgtttt tggcgtttat
     8761 ccgccagcgt ctgcgccacc tggtaataac cgctcaccgc atcgttggga ttaattgcca
     8821 gtacgccgca ctgggcatca agcactgcag gttgcccggc ataacgggca atcgcatcca
     8881 gcggcaggcc gctcagtacc gggatcgccg aagcgcgggc cagaatcagg gtatgcgagg
     8941 tgcgcccggt tttctccaga atcatgcccg caagattttt taaatcgagg ctcaaaaact
     9001 ggcttggggt taaatcttca gccaccagaa tggtcggttt ttcaagcacc agattattgc
     9061 gcggttttag ttccggccag gtgatatgca gcaactgttc gctgatatcg cgaatgtcgc
     9121 tgacccgctc gcgcagataa tcgctggcag aagcagaaag tttggcacaa atctgctcca
     9181 tattgctgat gatcgccgcc cccagcccct gatgctgttc tgccatcagg tgacggatat
     9241 tgcctgcaaa ttcatcatcc tgaatcagcg acaaatgggc gctgaggata gttttgcttt
     9301 cgccgtcacg ctcacgcaat tgctggttca gttgctcggc aagcgttgcc agactgtgct
     9361 ccagtagggt ggaatcttgc gcactcgcag ggatcacccg ataactgtcg aggctgtcgc
     9421 tctgtaacag ggtcagtgta cccacgccaa cgccgcttgc cagcacgttg ccgtacagta
     9481 aatccgggtt caggcggctt aatgaacgcg gcagcggatg cgccgtcagt tctgccagcg
     9541 tcggctggac gctatcgctg tcgataaagc gcacctggat atactcttcc agcactcgcc
     9601 gcgcctgctc ttcatcgcta ccgctaatgt tcaggctgca actgtcatta aataaagtgc
     9661 cagtgccaat cagcgccagc gagcttttcg catcggcctt tgcgttctgg cgatggttaa
     9721 taaaagtgat ttcgctttgc cactggctgc actgctcttt aagttcccac gccggacgag
     9781 catgtagacc gttaggcaaa ggacagagaa attgaatcgt taacataacc gctccgggtt
     9841 aatcaggaga tcatctgaaa tccgtcagtt gaacaaccgt ctcacgcgta agacgttgaa
     9901 taagggcaga taaaagttgc tgcatctgca aaatgtcgcg gcagtcggca atcgatgcgg
     9961 cgcaatgtcc gtggcgggtt gctggcccca tcaccactgt gggtacgcca gtgccggtta
    10021 agtgcaccgc cccaccgtcc gtgccgccat tgctgaacat atctgactgc aacggtacgc
    10081 caatttctgc cgccacggtt tcgatccagg cgataagttt tggcggcgca atcagtgact
    10141 tgtcgcttaa caccagcatc ggcccgttac caatctggcg atggttagct gcgccataat
    10201 caaagttttt cgcccagcag gcggtatcaa gcacaatggc gacatccggc gacaccgcgc
    10261 gggtggcagt ttgcccgccg cgtaatccca cctcttcgct ggaacttgcc accagccaca
    10321 cttccgcagg cagttcggcg tcgtgcagct cacgcagtaa cgtcaccagc agatagcaac
    10381 cgaggcggtc atcaaaggct ttccccatca cccgctggtg agggagaacc tgaaaagtgg
    10441 tatcgaaggt gacgcgatcg cctggacgaa ttcctgcctg catcacttcg tcacaggagc
    10501 gcgcgccaat gtccacgcgc atggcgctga cgtcattccc ctgccggtcg ccgtcaagca
    10561 ggcctggaat tttgcactct tcacgggtag tgatacgcac cgactgaagc tggcgggcag
    10621 ccatgcgtac attgccaacc ggcaacacat cgatcgcccc ttcgcgggag atgctgcgca
    10681 ccataaatcc cacttcgtcc atatgcgcac agatcatcac cttcggacct gtcgactcat
    10741 tgagtcggat cagcaccgat cccaggccgt caaaacggac ctctttttgc tggcgatccg
    10801 cttcatccag caggatctgc cgtacttcct gttccgagga ggcgatcgca tctgcctcgc
    10861 tcaacgcttt taagagcgat aaatccatta tgcctctccc gtgagcaaca ctgttttcgg
    10921 catggcgtag agcacttctg cgccttgcgg ggtgaccagc acgacatctt caatgcgcac
    10981 gcccccttgc cctggaaaat aaatccctgg ctccacggtc agtaacatac ctggctgtag
    11041 cgtcgtggtg tcccgcggtg aaaaacgcgg ctcttcatgg acttcaatac cgatagcatg
    11101 accggtgtta tggccgaaaa acttgccata accggcctcg gtaatcacgc ggcgcgcggc
    11161 atcgtcaacc tgctggcagc gcacgccggg gcgaattaca gagattgccg cgagctgtgc
    11221 ctgcaggaca atctgataga cgttaaacag cgggtgagat tcggcgctca ccccttcgcc
    11281 attcaccagc aaggtgcgcg tcatatcaga gcagtagccc tgatacagcg caccgaaatc
    11341 gagagtgaca aactcgcccg ctgcaacaat cttgtcgctg gctttaccgt gcggcagcgc
    11401 cccacgcaag ccactggcga caatggtgtc aaaagaggct ttttctgcgc cctgctggcg
    11461 cataaaccac tccagttcag cggctatctc gcattcgctc atacccgcct gaataaagcg
    11521 gcgaatatgc tctgcgccac gatcggcaat cccgcaggca aggcggattt tctccacctc
    11581 ttctggcgtt ttgatttgcc gcagcacatc cggcgtggcg ctcaccagtt tcgcattgag
    11641 ttcgaactgc cagcggtgcg cggtttccca actcacctgc tggccttcaa aaccgagcgt
    11701 ttgcaactgc tcatcggcaa tgatttgtct gacgatagtg gcaagcgtgt gcatcgcgtc
    11761 gagcaaatgc agctggtaac cttgcgtgcg ggcttctaca tccgcgtaat agcgcgaatc
    11821 caccagaatg tgcgcacttt cacggctaat taccacataa cctgatccgg tggagatccc
    11881 cagatgcggc tgtttgttct gccgtgagga gagaagcacc gcatccagtt gttgcgcctt
    11941 aagccagtcg cgcagcgaag cgagtaatgt catcaggatt gtccttctta caggctatcg
    12001 attaacaatt tgcctttacg gaacatcatc agacgcagga acaccaccat cagcgcagta
    12061 atgattgctc ccagcgcgat ccccgccata tagacgccga ggttagtgac cagcggccat
    12121 gcccagatag cggactccgg gaaccattgc actgcgccca gccagactgc ggcggttgag
    12181 ccgacaatcg cgccaaccat atacgacgga atggcggtga tagggctttc cagtgcaaac
    12241 ggaatcgcac cttcactgat ccccataaac gccaggaaca tcgcggtctt accctgcgga
    12301 taaagttgag cgttgaacag gcgtttgccg gttaaacgac ggtcaataat ggttgccaga
    12361 cccagaccaa tcggcggaat aacgatagcg atagaacgtg cggtgaccgg taatacgtgg
    12421 tcagtggtaa agctgaaggc aacgaaacct gccgctttgt tgatcgggcc accaaggtcg
    12481 attgctgtcg ccgcggcgat ccccattgcg tacatcagcg cgcccttctc acctgcagcg
    12541 gtcagcacgg tacggatacc gccgttgatc cagccaccaa agggggtgat gacgtagtac
    12601 atcgccagca tgacaaaaat ggcggaaaga atcggtagca aaaatgtggt tttgaacgct
    12661 aacaggaaat ccggtaactg gattttctgg ttcatccact tcacgaggta acccgcaacg
    12721 atagatataa tcagcgcacc aatgaaggtg gacggcaccg gcgaagaggt cgcccattgc
    12781 ataatgctgg gatcgaagtt cagcagttgc gtcggctggg tagacatcaa gccgccgata
    12841 aaaccagccg ggaacgccag tttgccaccg atagagttcg ccacaaaagc agcaaacatc
    12901 ggaatggcaa aaccaaacaa cacaccgccg aaggactgcg acagccaggc aaatttcagt
    12961 aaggagaggt cgaaaccgga gaatttcccg ctattaagag catccatgat gccgatatca
    13021 gcaggaattt taagccagct ataagcaatc agctgactga aagcgaggat cacaccgccc
    13081 ataattaagg tcggcaccat gcgtgaaatc cctgacatca catgctgcgg gagttcaccc
    13141 cagaagctgg atttagaggc ctgcggattc ttaactgccg ctgccgcacc ggatgcgcca
    13201 tgcacaacgg ttgcactgcg ttttttaatg gccataacaa ttatccctgt cgaatattac
    13261 tgttgttcag aggcaatcat ctcttcgatt tctttgatga ttcctgcagc gtttttaatt
    13321 gcgtcctgca aagtgatttc ataaacgtcg cgtgattcga aacgctcgtt atcttccggg
    13381 gtaactgcca cggagtggat gatgatggtc gcttcggcga tatcctgcgc cgtcaggcga
    13441 ttctggatac cgtccgcgcc ctgggtttca attttcactt cataaccggc ttctaccgcc
    13501 gcttcttcca gcgcctgagc ggccataaaa gtgtgagcca ggcccattgg gcaggcacat
    13561 aaggcaatca gtttcttact catcgttaat tcctcgttca gtgatagttg acgagtgaaa
    13621 ttatgaacgt gcaaaacaaa tcgccgttac cagacaaata atgcatttac tggagaatta
    13681 atcccgctgt acggaagtgt gacgcaggtc ggccttgtga ggggacacaa agaaaaattt
    13741 aattaactgt atgatttaaa agattatcgg gagagttacc tcccgatata acaggaagga
    13801 tttagagaat gtgacctaag gtctggcgta aatgtgcacc ggaaccgaga agacccggat
    13861 tgtcatggac gatgagatac accggaatat catggacata ttctttaaag cgccctttat
    13921 cttcaaatgc ggcgcggaaa ccagaggctt tgaagaactc aaggaagcgc ggcacgatac
    13981 cacccgcaat aaacacgccg ccaaatgtcc cgagattgag cgccagattg ccgccaaaac
    14041 ggcccataat gacgcaaaac agcgacaacg cgcggcggca atcggtgcag ctgtcagcca
    14101 gcgcgcgttc ggtaatatct tttggcttga gattttctgg caggcggttg tcggctttaa
    14161 caattgcgcg atacaaattc accagtccag ggccagaaag cacgcgttcc gccgaaacat
    14221 gaccaatttc cgcacgcaat atttcgagga taatgccctc ttcttcacta ttcggcgcaa
    14281 aatcaacgtg accgccttct cctggcaagc ttacccaacg cttatcgaca tggaccagat
    14341 gcgcaacccc aagccctgtt ccggcaccgt aaaccgcaat aggcttacct tctaccggtt
    14401 ctgcgccacc aaactgaatc agatgctctt ttttcagcat cgggatcgcc atcgatacag
    14461 cagtaaaatc gttaataatt tccagatggc taaaaccgag attctttttc atttcggcaa
    14521 ttgagaacgc ccaggtatgg ttggtcattg ccacccagtc accggtaatt gggcaagcga
    14581 tggcaataca gccgtctttc acctcgacct tatgttcttc aagataaacg cgaatgaccg
    14641 cttcgaggct ggggtaatca agccctgaat aggtcttagc ctgcgagatt tcaccactgg
    14701 caatatcaca cagagcaaga cgtgcgttgg tgccgcccac atcaccgact aatgcatact
    14761 ttgtcattct tcaactgctc cgctaaagtc aaaataattc tttctcacac tgtaaatacc
    14821 tgggggcata acaacaacgc cggaaaggca ggctccctgt aaatatcgat ctgggtcaca
    14881 caattacttt atcgtttcag caccaattgc agcgatgcct ttttgcaagc tgggcaaact
    14941 aagtatctga ccccgcataa ggaatagaac atgctccatc cgcgagccag aaccatgttg
    15001 ttattatcgc tccccgccgt ggcaattggg attgcgtcca gtcttattct gattatggtg
    15061 atgaaaatcg cctcggtatt acagaatttg ctctggcaac gactgccggg aactctgggg
    15121 atagcccagg attcacctct ctggatcatc ggtgtattaa cgctaacggg tattgcggtg
    15181 gggttggtta tccgtttcag ccagggtcat gccggaccag atcccgcctg tgaaccgctt
    15241 atcggcgcac cgattccgcc ctctgcgcta ccaggactta tcgtagcatt aattctcggt
    15301 cttgctggcg gcgtcagcct ggggccggaa catccgatca taaccgtcaa tattgccctt
    15361 gccgtcgcca ttggcgctcg tctgttaccg cgcgtcaacc gaatggagtg gactatttta
    15421 gcctctgccg gaaccatcgg cgcgctgttt ggcacacctg ttgcggcggc attgatattt
    15481 tcgcaaacct taaatggcag taatgaagtt ccgctatggg atcgtctctt tgcgccgtta
    15541 atggcggcag cagctggtgc acttactacc ggattatttt tccatcctca tttttcactg
    15601 cccattgcgc attacggaca gatggaaatg accgatattc tcagcggtgc aattgtcgcg
    15661 gcgattgcca tcgcagcagg gatggtcgcc gtatggtgct taccacggtt gcacgcgatg
    15721 atgcatcaaa tgaaaaatcc ggtgttcgtg ctgggtattg gcggatttat tctcggtatt
    15781 ctgggggtta ttggtggacc ggtttcgctg tttaaagggc tggatgagat gcagcagatg
    15841 gtggcaaatc aggctttcag caccagcgat tactttttgc tggcggtgat aaaacttgcc
    15901 gccctggtcg ttgctgccgc cagtggcttt cgcggtgggc gaatcttccc ggcagtgttt
    15961 gtcggcgtgg cattagggtt gatgcttcat gagcacgttc ccgccgttcc agcggcaata
    16021 accgtttcct gcgctatact cggcatcgtg ctggtggtaa cacgcgatgg ctggttaagt
    16081 ctttttatgg cggcagtcgt tgtacccaat accacattgc taccactgct ctgtatcgtc
    16141 atgcttccgg catggctgtt attagcaggt aagccgatga tgatggtcaa tcgtccgaag
    16201 caacagccac cccacgataa catttagcaa aaaatgctct cctttatgat ttaagagtta
    16261 tggcgatgat acggggcttt acctccccgt aatattgcgt taacaggccg ctgacggctt
    16321 cgatcgtgaa ggagaataac aatgttcagg tcactgtttc tggcggccgc cctgatggca
    16381 tttaccccgc ttgcggcaaa cgcaggtgaa atcaccctac tgccatcaat caaattacaa
    16441 attggcgatc gcgatcatta cggtaattac tgggacggtg gtcactggcg cgaccgtgac
    16501 tactggcatc gcaattatga atggcgtaaa aaccgctggt ggcgtcatga taatggctac
    16561 caccgtggct gggataagcg taaagcgtat gagcgtggct atcgtgaagg ctggcgcgat
    16621 cgtgacgatc atcgcggaaa aggccgcgga catggacacc gccattaaac acattcgcca
    16681 atggagcaca atgcctgatg cgacgctagt gcgtcttatc aggcctacaa accggctctt
    16741 taacgcgtag ggcggataag gcgtttacgc cgcatccgcc aatagtgcca gatgcgacgc
    16801 tcattcaact acaatcccag cgccgtcccc accaacaacc agatattcag cgccacgacc
    16861 agcactacaa tcacccagcc tgtctgtttt acgcgtttgc tgttcaccag atcgcccatc
    16921 aacttgctgt cactggtgaa aatcagcagt ggaaccagcg ccagagcgat accaaaactt
    16981 aacagcacct gactcataac cagaatccgt gtcggatcta atcccatcag aatgacaata
    17041 aatgacggca gcatggtgac tgtacgacgc acccacagcg ggatatgaaa gcgaatgaag
    17101 ccctgcatca ctacctgccc cgccagcgtc cccaccaccg ttgaggacaa cccggcagca
    17161 accaggctta atccaaagac cgttgccgca gcatggctta acagcggttg cagcgtcaga
    17221 taagcctcat caagatcggc aacaccagta tgaccagaaa agtggaacgc cgccgcagct
    17281 gtagccatca tcgccagatt aacaaaaccg gcaatcgtca tggcgatagc cacatcccat
    17341 ttggtggcgg aataacgttg ttgacgcgaa ccgccatgta aatgctgagt gagcgaagag
    17401 tgcaaataaa tcacatgcgg cataatcgtc gcccctaaca cgcccgctgc caggaagacc
    17461 gcctccgaag taggtaaact cgggatcacc attcctttac ccagctgcgc caggttaggc
    17521 tgggagaaaa tcaactcgac aatgtaagca gcggcaacaa acaacagtaa cccgccaatc
    17581 actttctcca gcggtttttg cccgcgacgt tgcagcatta aaataaggaa agtcgcgatc
    17641 cccgtcagca ccgcgccctg caacaacgaa acaccaagaa tgagtttaaa accgatcgcc
    17701 gcaccaataa attccgccag gtcggttgcc atcgcaataa tttctgcctg aacccaatag
    17761 aaccacacga cgggacgcgg gtagtgatcg cgaatctgct ccgccagatt tttaccggtg
    17821 gcaatcccca gtttggccga gaggatttga atcagcatcg ccatcaggtt agcccaaacg
    17881 acaacccaca ataactgata gccgaagctg gcacccgcct gaatattggt cgcaaagtta
    17941 ccgggatcga tataaccaat cgccgcaatg aacgcaggtc ccattaatgc gagcctcatc
    18001 ttgcgcgccg cccgtccgct gctactctca acgcgatagt tcgtcatctt gtgcctctaa
    18061 aacatagcct ttgctatgtt tcatgctacg ttaaatgaga atgattatca aattcattta
    18121 aatgggttgt gatgatttct ctgatagacc aggattatga ctacgaaaag atagatgact
    18181 cgaatgttat ggttatgttt aatattagca catttacata accttccgct tccatacaca
    18241 acatagcaga aatgtatgac agatcactat ttttgaagct tgtcacagga cgtcattata
    18301 gtgtgtgtca gatctcgttt tccttaacca tgttacatag aatgtgcacg gaaatttaac
    18361 ctgcctcata tttggagcaa atatggaccg cgtccttcat tttgtactgg cacttgccgt
    18421 tgttgcgatt ctcgcactgc tggtaagcag cgaccgcaaa aaaattcgta tccgttatgt
    18481 tattcaactg cttgttatcg aagtgttact ggcgtggttc ttcctgaact ccgacgttgg
    18541 tctgggcttc gtgaaaggct tctccgaaat gttcgaaaaa ctgctcggat ttgccaacga
    18601 agggactaac ttcgtctttg gtagcatgaa tgatcaaggc ctggcattct tcttcctgaa
    18661 agtgctgtgc ccaatcgtct ttatctctgc gctgatcggt attctccagc atattcgcgt
    18721 attgccggtg attatccgcg caattggttt cctgctctcc aaagtcaacg gcatgggcaa
    18781 actggaatcc tttaacgccg tcagctctct gattctgggt cagtctgaaa actttattgc
    18841 ctataaagat atcctcggca aaatctcccg caatcgtatg tacaccatgg cagcaacggc
    18901 gatgtccacc gtgtcgatgt ccatcgttgg tgcatacatg accatgctgg agccgaaata
    18961 cgtcgttgcg gcgctggtac tgaacatgtt cagcaccttt atcgtgctgt cgctgatcaa
    19021 cccttaccgt gttgatgcca gtgaagaaaa cattcagatg tccaacctgc acgaaggtca
    19081 gagcttcttc gaaatgctgg gtgaatacat tctggcaggt ttcaaagttg ccattatcgt
    19141 tgccgcgatg ctgatcggct ttatcgccct gatcgccgcg ctgaacgcac tgtttgccac
    19201 cgtgactggc tggtttggct acagcatctc cttccagggc atcctgggct acatcttcta
    19261 tccgattgca tgggtgatgg gtgttccttc cagtgaagca ctgcaagtgg gcagtatcat
    19321 ggcgactaaa ctggtttcca acgagttcgt tgcgatgatg gatctgcaga aaattgcttc
    19381 cacgctctct ccgcgtgcgg aaggcatcat ctctgtgttc ctggtttcct tcgctaactt
    19441 ctcttcaatc gggattatcg cgggtgcggt taaaggcctg aatgaagagc aaggtaacgt
    19501 ggtttctcgc ttcggtctga aactggttta cggctctacc ctggtaagtg tgctgtctgc
    19561 gtcaatcgcg gcactggtgc tgtaagacca tacataaaaa agccggggat aattccccgg
    19621 cttttttacg cctgttaatc agctaatggc tgcgggcgac caatcaaata cccttgcaga
    19681 tattgcaccc caagtttatg caatagcgcc tgctgctgtg gcgtctcgac aaactccgcg
    19741 accacactca atgacttcgc tttcgccaga tcggtaattg atctcacaat catcgcatcc
    19801 agcgtgttcg tgacgatatc tttcacaaag acgccatcaa ttttgatgat atcagcctgc
    19861 aaacgcttta accgttcgta gttggcatat ccggtgccaa agtcatcaat cgcaatccgg
    19921 aaaccaaact tatgcagctg ctcgatgtta tacatgctgc tttctgcgtt agaaaacgcc
    19981 tgctcctcgg tgatctcgag aatgaccgcc tgcggggaga tgtgataacg cttaaacaga
    20041 cgaataatcc gcccggcaat attcttttgc agcagcgtga gcggcattaa attaactgaa
    20101 aatcgtgggc cttttttgtc gcaagggtgt gttgccagcc atttcaacaa ggattccagc
    20161 acttgcaaat caaaacgcgc gctaaggtta aactgagcaa taaggggcag aaacttatcc
    20221 ggggtcataa tgccgccgtc atacttcagt cgcgcgagga tctcatcata accttcaccc
    20281 tctttgttgc gaattggctg ggcgtagagc agcaaatccc cctgatctaa cgccgtgcga
    20341 atggtattca gcaatagcac ctgtttagtg gtttgcccgg aaaccatctc ctctctgcta
    20401 tccagcgcca gcacatgatg atgtgcgcag gattgctccg ccagccagct taactgcccc
    20461 aacaagggtt gcagggtttc ctgatttcca tcaaaacgtc cccaggcagc accatagccc
    20521 atatccagcc cggtattgtt ccagtgaatt tgccgactat tcaggatgtt aaccatatgc
    20581 tggagtcgcc cttccgtttc cggcccgctc agcactaaca gcagttcact acccggcaat
    20641 tgatacaact tttcgttttc ctgcatcaac ggcagcagcg tacggtaaat tgagcggata
    20701 cagtgaacgc gcatcattaa gccgtaatga cgactcataa actcaagatt atcaatgcgc
    20761 aggcagcaaa aactcttgcc cgcctcttgc tccggcgctt gctccaacgc acgaaagttg
    20821 ggtagaagcg ttaacggatc ggtcagcgcc tgcaaatgcc agcggcggtt aagccattca
    20881 ctacgatgat aaatacgcac catatagagc aggcaaacac taaaggaaat cagtactgcg
    20941 agaataaatg ccagcgaata ttcggttagt accccttgca aaaagttctg gttgtaattc
    21001 agaaggcaaa gcgttgaaac agcccaggtg agatttaaaa acggatagcg aagcttaccg
    21061 acaccgaggg taaagatgat aaagaaaaca ggtaccaggt aaccggcaat aaagtcattt
    21121 tcatacggtg tgcacatcag aagtagcagc acgctaagag ctgccagcca gcttaaggta
    21181 aatgcgcgtt tctctttgct caacgacgga gcgatatccc tacgccacaa tatctgtgca
    21241 aagtggggac ttacaatcat gcgggcgaga tagtaaaaaa gcatgttgta aatcagcact
    21301 gcggtgaaaa ggcttagcaa atcaacgacc gtgaaaatgg catccgcatc gccgaaaaag
    21361 gtagatatct ttagcggaaa atcaaagaaa tttcccacaa gatacatgct gcatttgatg
    21421 ccaatcggtg ccaccaaacc aagccagaac agacgttgcc agacgtatcg gctggtcaat
    21481 ccataacgcc aacgcgtccc caattgccag cgtaatattg cacaggcaca caggactgca
    21541 aacgcctggc tgccgatcat gacggcggtc tgtaaaaaag agagatgcag gttccacaga
    21601 ttggtgcaaa acatcccgca taaaatggga acgacaccac gccagccaaa gataaacaag
    21661 attgacagca ttacgcacag tggcatccac gccagaaaga tataattgga atggataaca
    21721 gcaagtggcg aaattaaacg ggatagctga ataagtacca cggtgagcgt aaacgctagt
    21781 gtgaatatct tgatattttt tatcaggtta tgctccacaa acatgagtac actactctca
    21841 ctgatcgggg ttttcaggca cacaaataat aagtttattc gcatcgcgaa gcaaacaggg
    21901 gccagcctgt tgctgttttt tcagtcagtg atgcattcac cacacggcac tgccaggatg
    21961 tagactgtat tacaaaagcg gcaaaaggca gagacaaaaa acccccgctt tgcagcgagg
    22021 gtttgaaatt tggtggagct aagcgggatc
//
LOCUS       DADSYM010000074        21640 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2415
            SAMN08039579-rid11723173.denovo.074, whole genome shotgun sequence.
ACCESSION   DADSYM010000074 DADSYM010000000
VERSION     DADSYM010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039579
            Sequence Read Archive: SRR6362533
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 21640)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21640)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980162.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 03:39:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,484
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 4,347
            CDSs (with protein)               :: 4,347
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 43 of 85
            Pseudo Genes (incomplete)         :: 37 of 85
            Pseudo Genes (internal stop)      :: 19 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21640
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039579-rid11723173.denovo.074"
                     /strain="LGE2415"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8302 N 95.9628 W"
                     /collection_date="2015-08-24"
     gene            complement(29..313)
                     /locus_tag="J1Y83_002451"
     CDS             complement(29..313)
                     /locus_tag="J1Y83_002451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004088972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBA7630595.1"
                     /translation="MSFFDELKTSLEEAVEIKQGLKEPARVTRYEIADVKAIREQLNV
                     SQSEMAKALGTSVDTIKSWESKRRNPTGLAAKVLATIKENPAFFRELASH"
     gene            complement(315..647)
                     /locus_tag="J1Y83_002452"
     CDS             complement(315..647)
                     /locus_tag="J1Y83_002452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015873600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HBA7630596.1"
                     /translation="MGKAIIFIETPMFTRQIKQIATDDELKELQKELIGTPDKGDLIQ
                     QTGGLRKVRMAAGSQGKSGSVRIIYSLATEEIIYLIMAYPKNAKDSLTDTEKAQLKKL
                     TKLLKGEI"
     gene            complement(739..1971)
                     /locus_tag="J1Y83_002453"
     CDS             complement(739..1971)
                     /locus_tag="J1Y83_002453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418807.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trifunctional nicotinamide-nucleotide
                     adenylyltransferase/ribosylnicotinamide
                     kinase/transcriptional regulator NadR"
                     /protein_id="HBA7630597.1"
                     /translation="MSSFDYLKTAIKQQGCTLQQVADASGMTKGYLSQLLNAKIKSPS
                     AQKLEALHRYLGLEFPRQKKTIGVVFGKFYPLHTGHIYLIQRACSQVDELHIIMGFDD
                     TRDRALFEDSAMSQQPTVPDRLRWLLQTFKYQKNIRIHAFNEEGMEPYPHGWDVWSNG
                     IKKFMAEKGIQPDLIYTSEEADAPQYMEHLGIETVLIDPKRTFMSISGAQIRENPFRY
                     WEYIPTEVKPFFVRTVAILGGESSGKSTLVNKLANIFNTTSAWEYGRDYVFSHLGGDE
                     IALQYSDYDKIALGHAQYIDFAVKYANKVAFIDTDFVTTQAFCKKYEGREHPFVQALI
                     DEYRFDLVILLENNTPWVADGLRSLGSSVDRKEFQNLLVEMLEENNIEFVRVEEDDYD
                     SRFLRCVELVREMMGEQR"
     gene            complement(1992..3374)
                     /gene="radA"
                     /locus_tag="J1Y83_002454"
     CDS             complement(1992..3374)
                     /gene="radA"
                     /locus_tag="J1Y83_002454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001399683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HBA7630598.1"
                     /translation="MAKAPKRAFVCNECGADYPRWQGQCSACHAWNTITEVRLAASPT
                     VARNERLSGYAGSAGVAKVQKLSDISLEELPRFSTGFKEFDRVLGGGVVPGSAILIGG
                     NPGAGKSTLLLQTLCKLAQQMKTLYVTGEESLQQVAMRAHRLGLPTDNLNMLSETSIE
                     QICLIAEEEQPKLMVIDSIQVMHMADVQSSPGSVAQVRETAAYLTRFAKTRGVAIVMV
                     GHVTKDGSLAGPKVLEHCIDCSVLLDGDADSRFRTLRSHKNRFGAVNELGVFAMTEQG
                     LREVSNPSAIFLSRGDEVTSGSSVMVVWEGTRPLLVEIQALVDHSMMANPRRVAVGLE
                     QNRLAILLAVLHRHGGLQMADQDVFVNVVGGVKVTETSADLALLLAMVSSLRDRPLPQ
                     DLVVFGEVGLAGEIRPVPSGQERISEAAKHGFRRAIVPAANVPKKAPEGMQIFGVKKL
                     SDALSVFDDL"
     gene            complement(3423..4391)
                     /gene="serB"
                     /locus_tag="J1Y83_002455"
     CDS             complement(3423..4391)
                     /gene="serB"
                     /locus_tag="J1Y83_002455"
                     /EC_number="3.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine phosphatase"
                     /protein_id="HBA7630599.1"
                     /translation="MPNITWCDLPEDVSLWPGLPLSLSGDEVMPLDYHAGRSGWLLYG
                     RGLDKQRLTQYQSKLGAAMVIVAAWCVEDYQVIRLAGSLTARATRLAHEAQLDVAPLG
                     KIPHLRTPGLLVMDMDSTAIQIECIDEIAKLAGTGERVAEVTERAMRGELDFTASLRS
                     RVATLKGADANILQQVRENLPLMPGLTQLVLKLETLGWKVAIASGGFTFFAEYLRDKL
                     RLTAVVANELEIMDGKFTGNVIGDIVDAQYKAKTLTRLAQEYEIPLAQTVAIGDGAND
                     LPMIKAAGLGIAYHAKPKVNEKTEVTIRHADLMGVFCILSGSLNQK"
     gene            4497..5141
                     /locus_tag="J1Y83_002456"
     CDS             4497..5141
                     /locus_tag="J1Y83_002456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtjB family periplasmic protein"
                     /protein_id="HBA7630600.1"
                     /translation="MARTKLKFRLHRAVIVLFCLALLVALMQGASWFSQNHQRQRNPQ
                     LEELARTLARQVTLNVAPLMRTDSPDEKRIQAILDQLTDESRILDAGVYDEQGDLIAR
                     SGESVDVRDRLALDGKKAGGYFNQQIVEPIAGKNGPLGYLRLTLDTHTLATEAQQVDN
                     TTNILRLMLLLSLAIGVVLTRTLLQGKRTRWQQSPFLLTASKPVPEEEESEKKE"
     gene            5169..6185
                     /gene="lplA"
                     /locus_tag="J1Y83_002457"
     CDS             5169..6185
                     /gene="lplA"
                     /locus_tag="J1Y83_002457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase LplA"
                     /protein_id="HBA7630601.1"
                     /translation="MSTLRLLISDSYDPWFNLAVEECIFRQMPATQRVLFLWRNADTV
                     VIGRAQNPWKECNTRRMEEDNVRLARRSSGGGAVFHDLGNTCFTFMAGKPEYDKTIST
                     SIVLNALNALGVSAEASGRNDLVVKTAEGDRKVSGSAYRETKDRGFHHGTLLLNSDLS
                     RLANYLNPDKKKLAAKGITSVRSRVTNLTELLPGITHEQVCEAITEAFFAHYGERVEA
                     EIISPDKTPDLPNFAETFARQSSWEWNFGQAPAFSHLLDERFTWGGVELHFDVEKGHI
                     TRAQVFTDSLNPAPLEALAGRLQGCLYRADMLQQECEALLVDFPEQEKELRELSAWMA
                     GAVR"
     gene            6217..6480
                     /locus_tag="J1Y83_002458"
     CDS             6217..6480
                     /locus_tag="J1Y83_002458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBA7630602.1"
                     /translation="MIDLENQEREIINLMFSQGISWLTAVRIRHKLSLAEVSKMLGIS
                     INSLKQIEKTERLSSNIKSKMAGIYGCSPELLICPSWMTAEHK"
     gene            complement(6641..7360)
                     /gene="deoD"
                     /locus_tag="J1Y83_002459"
     CDS             complement(6641..7360)
                     /gene="deoD"
                     /locus_tag="J1Y83_002459"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710124.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HBA7630603.1"
                     /translation="MATPHINAEMGDFADVVLMPGDPLRAKYIAETFLEDAREVNNVR
                     GMLGFTGTYKGRKISVMGHGMGIPSCSIYTKELITDFGVKKIIRVGSCGAVLPHVKLR
                     DVVIGMGACTDSKVNRIRFKDHDFAAIADFDMVRNAVDAAKALGVDARVGNLFSADLF
                     YSPDGEMFDVMEKYGILGVEMEAAGIYGVAAEFGAKALTICTVSDHIRTHEQTTAAER
                     QTTFNDMIKIALESVLLGDKE"
     gene            complement(7417..8640)
                     /gene="deoB"
                     /locus_tag="J1Y83_002460"
     CDS             complement(7417..8640)
                     /gene="deoB"
                     /locus_tag="J1Y83_002460"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HBA7630604.1"
                     /translation="MKRAFIMVLDSFGIGATEDAERFGDVGADTLGHIAEACAKGEAD
                     HGRKGPLNLPNLTRLGLAKAHEGSTGFIPAGMDGNAEVIGAYAWAHEMSSGKDTPSGH
                     WEIAGVPVLFEWGYFSDHENSFPQELLDKLVERANLPGYLGNCHSSGTVILDQLGEEH
                     MKTGKPIFYTSADSVFQIACHEETFGLDKLYELCEIAREELTNGGYNIGRVIARPFIG
                     DKAGNFQRTGNRHDLAVEPPAPTVLQKLVDEKHGQVVSVGKIADIYANCGITKKVKAT
                     GLDALFDATIKEMKEAGDNTIVFTNFVDFDSSWGHRRDVAGYAAGLELFDRRLPELMS
                     LLRDDDILILTADHGCDPTWTGTDHTREHIPVLVYGPKVKPGSLGHRETFADIGQTLA
                     KYFGTSDMEYGKAMF"
     gene            complement(8692..10014)
                     /gene="deoA"
                     /locus_tag="J1Y83_002461"
     CDS             complement(8692..10014)
                     /gene="deoA"
                     /locus_tag="J1Y83_002461"
                     /EC_number="2.4.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313368.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine phosphorylase"
                     /protein_id="HBA7630605.1"
                     /translation="MFLAQEIIRKKRDGHALSDDEIRFFINGIRDNTISEGQIAALAM
                     TIFFHDMTMPERVSLTMAMRDSGTVLDWKSLHLNGPIVDKHSTGGVGDVTSLMLGPMV
                     AACGGYIPMISGRGLGHTGGTLDKLESIPGFDIFPDDNRFREIIKDVGVAIIGQTSSL
                     APADKRFYATRDITATVDSIPLITASILAKKLAEGLDALVMDVKVGSGAFMPTYELSE
                     ALAEAIVGVANGAGVRTTALLTDMNQVLASSAGNAVEVREAVQFLTGEYRNPRLFDVT
                     MALCVEMLISGKLAKDDAEARAKLQAVLDNGKAAEVFGRMVAAQKGPTDFVENYAKYL
                     PTAMLTKAVYADTEGFVSEMDTRALGMAVVAMGGGRRQASDTIDYSVGFTDMARLGDQ
                     VDGQRPLAVIHAKDENSWQEAAKAVKAAIKLADKAPESTPTVYRRISE"
     gene            complement(10092..10871)
                     /gene="deoC"
                     /locus_tag="J1Y83_002462"
     CDS             complement(10092..10871)
                     /gene="deoC"
                     /locus_tag="J1Y83_002462"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005097947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HBA7630606.1"
                     /translation="MTDLKASSLRALKLMDLTTLNDDDTDEKVIALCHQAKTPVGNTA
                     AICIYPRFIPIARKTLKEQGTPEIRIATVTNFPHGNDDIEIALAETRAAIAYGADEVD
                     VVFPYRALMAGNEQVGFDLVKACKEACAAANVLLKVIIETGELKDEALIRKASEISIK
                     AGADFIKTSTGKVAVNATPESARIMMEVIRDMGVEKTVGFKPAGGVRTAEDAQKYLAI
                     ADELFGADWADARHYRFGASSLLASLLKALGHGDGKSASSY"
     gene            11129..12679
                     /locus_tag="J1Y83_002463"
     CDS             11129..12679
                     /locus_tag="J1Y83_002463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjjI family glycine radical enzyme"
                     /protein_id="HBA7630607.1"
                     /translation="MPTSHENALQQRCQQIVTSPVLSPEQKRHFLALEAENNLPYPAL
                     PAEARRALDEGVICDMFEGHAPYKPRYVLPDYARFLAHGSEWLELEGAKDLDDALSLL
                     TILYHHVPSVTSMPVYLGQLDALLQPYVRILTQDEIDIRIKRFWRYLDRTLPDAFMHA
                     NIGPSDSPITRAILRADAELKQVSPNLTFIYDPDITPDDLLLEVAKNICECSKPHIAN
                     GPVHDKIFTKGGYGIVSCYNSLPLAGGGSTLVRLNLKAIAERSESLEDFFTRTLPHYC
                     QQQIAIIDARCEFLYQQSHFFENSFLVKEGLINPERFVPMFGMYGLAEAVNLLCEKEG
                     IAARYGKEAAANEVGYRISAQLAEFVANAPVKYGWQKRAMLHAQSGISSDIGTTPGAR
                     LPYGDEPDPITHLQTVAPHHAYYYSGISDILTLDETIKRNPQALVQLCLGAFKAGMRE
                     FTANVSGNDLVRVTGYMVRLSDLEKYRAEGSRTNTTWLGEEAARNTRILERQPRVISH
                     EQQMRFSQ"
     gene            12651..13514
                     /gene="yjjW"
                     /locus_tag="J1Y83_002464"
     CDS             12651..13514
                     /gene="yjjW"
                     /locus_tag="J1Y83_002464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjjW family glycine radical enzyme activase"
                     /protein_id="HBA7630608.1"
                     /translation="MNSRCALVSKIIPFSCVDGPGSRLALFLQGCNLRCKNCHNPWTM
                     GRCNDCGECVPQCPHQALQIVDGKVAWSAAVCEQCDTCLKMCPQHATPMAQSMSVDEV
                     LNHVRKAVLFIEGITVSGGEATTQLPFVVALFTAIKNDPQLCHLTCLVDSNGMLSETG
                     WEKLLPVCDGAMLDLKAWGSECHQQLTGRDNQQIKRSICLLAERGKLAELRLLVIPGQ
                     VDYLQHIEELAALIKGLGDVPVRLNAFHAHGVYGEAQSWPSATPEDVEQLADALRERG
                     VSRLIFPALYL"
     gene            complement(13731..14510)
                     /locus_tag="J1Y83_002465"
     CDS             complement(13731..14510)
                     /locus_tag="J1Y83_002465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026291.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HBA7630609.1"
                     /translation="MICRFIDTHCHFDFPPFSGDEEASLQRAAQAGVGKIIVPATEAE
                     NFARVQALAEKYQPLYAALGLHPGMLEKHSDVSLDQLQQALERHPAKVVAVGEIGLDL
                     FGDDPQFERQQWLLDEQLKLAKRYDLPVILHSRRTHDKLAMHLKRHDLPCTGVVHGFS
                     GSLQQAERFVQLGYKIGVGGTITYPRASKTRDVIAKLPLASLLLETDAPDMPLNGFQG
                     QPNRPEQAARVFAVLCELRPEPADEIAEVLLNNTYTLFNVP"
     gene            complement(14507..15580)
                     /locus_tag="J1Y83_002466"
     CDS             complement(14507..15580)
                     /locus_tag="J1Y83_002466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin family protein"
                     /protein_id="HBA7630610.1"
                     /translation="MGQRIPVTLGNIAPLSLRPFQPGRIALVCEGGGQRGIFTAGVLD
                     EFMRAQFNPFDLYLGTSAGAQNLSAYICNQPGYARKVIMRYTTKREFFDPLRFVRGGN
                     LIDLDWLVEATASQMPLQMDTAARLFDSGKSFYMCACRQDDYAPNYFLPTKQNWLDVI
                     RASSAIPGFYRSGVSLEGINYLDGGISDAIPVKEAARQGAKTLVVIRTVPSQMYYTPQ
                     WFKRMERWLGDSSLQPLVNLVQHHETSYREIQQFIEKPPGQLRIFEIYPPKPLHSIAL
                     GSRIPALREDYKLGRLCGRYFLATVGKLLTEKAPLSRHLVPVVTPESIVIPPAPVAND
                     TLVAEVSDAPQANDPTFNNEDLA"
     gene            complement(15702..15863)
                     /locus_tag="J1Y83_002467"
     CDS             complement(15702..15863)
                     /locus_tag="J1Y83_002467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002887716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1328 domain-containing protein"
                     /protein_id="HBA7630611.1"
                     /translation="MFRWGIIFLVIALIAAALGFGGLAGTAAGAAKIVFVVGIILFLV
                     SLFMGRKRP"
     gene            complement(15990..16595)
                     /gene="osmY"
                     /locus_tag="J1Y83_002468"
     CDS             complement(15990..16595)
                     /gene="osmY"
                     /locus_tag="J1Y83_002468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone OsmY"
                     /protein_id="HBA7630612.1"
                     /translation="MTMTRLKISKTLLAVMLTSAVATGSAYAENNAQTTNESAGQKVD
                     SSMNKVGNFMDDSAITAKVKAALVDHDNIKSTDISVKTDQKVVTLSGFVESQAQAEEA
                     VKVAKGVEGVTSVSDKLHVRDAKEGSVKGYAGDTATTSEIKAKLLADDIVPSRHVKVE
                     TTDGVVQLSGTVDSQAQSDRAESIAKAVDGVKSVKNDLKTK"
     gene            complement(16988..18577)
                     /gene="prfC"
                     /locus_tag="J1Y83_002469"
     CDS             complement(16988..18577)
                     /gene="prfC"
                     /locus_tag="J1Y83_002469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HBA7630613.1"
                     /translation="MTLSPYLQEVAKRRTFAIISHPDAGKTTITEKVLLFGQAIQTAG
                     TVKGRGSNQHAKSDWMEMEKQRGISITTSVMQFPYHDCLVNLLDTPGHEDFSEDTYRT
                     LTAVDCCLMVIDAAKGVEDRTRKLMEVTRLRDTPILTFMNKLDRDIRDPMELLDEVEN
                     ELKIGCAPITWPIGCGKLFKGVYHLYKDETYLYQSGKGHTIQEVRIVKGLNNPDLDAA
                     VGEDLAQQLRDELELVKGASNEFDKELFLAGEITPVFFGTALGNFGVDHMLDGLVEWA
                     PAPMPRQTDTRTVEASEDKFTGFVFKIQANMDPKHRDRVAFMRVVSGKYEKGMKLRQV
                     RTAKDVVISDALTFMAGDRSHVEEAYPGDILGLHNHGTIQIGDTFTQGEMMKFTGIPN
                     FAPELFRRIRLKDPLKQKQLLKGLVQLSEEGAVQVFRPISNNDLIVGAVGVLQFDVVV
                     ARLKSEYNVEAVYESVNVATARWVECADAKKFEEFKRKNESQLALDGGDNLAYIATSM
                     VNLRLAQERYPDVQFHQTREH"
     gene            complement(18668..19345)
                     /gene="yjjG"
                     /locus_tag="J1Y83_002470"
     CDS             complement(18668..19345)
                     /gene="yjjG"
                     /locus_tag="J1Y83_002470"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine 5'-nucleotidase"
                     /protein_id="HBA7630614.1"
                     /translation="MKWDWIFFDADETLFTFDSFTGLQRMFLDYSVTFTAEDFQDYQA
                     VNKPLWVDYQNGVITSLQLQHGRFESWAERLNVEPGKLNEAFINAMAEICTPLPGAVS
                     LLNAIRGNAKIGIITNGFSALQQVRLERTGLRDYFDLLVISEEVGVAKPNKKIFDYAL
                     EQAGNPDRSRVLMVGDTAESDILGGINAGLATCWLNAHNREQPEGIAPTWTVSSLHEL
                     EQLLCKH"
     gene            complement(19360..19806)
                     /gene="rimI"
                     /locus_tag="J1Y83_002471"
     CDS             complement(19360..19806)
                     /gene="rimI"
                     /locus_tag="J1Y83_002471"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HBA7630615.1"
                     /translation="MNTISSLETTDLPAAFHIEQRAHTFPWSEKTFASNQGERYLNFQ
                     LTQNGKMAAFAITQVVLDEATLFNIAVDPDYQRQGLGRVLLEHLIDELEKRGVATLWL
                     EVRASNAAAIALYESLGFNEATIRRNYYPTTDGREDAIIMALPISM"
     gene            complement(19775..20188)
                     /gene="holD"
                     /locus_tag="J1Y83_002472"
     CDS             complement(19775..20188)
                     /gene="holD"
                     /locus_tag="J1Y83_002472"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit psi"
                     /protein_id="HBA7630616.1"
                     /translation="MTSRRDWQLQQLGITQWSLRRPGALQGEIAIAIPPHVRLVMVAN
                     DLPALTDPLVSDVLRALTVSPDQVLQLTPEKIAMLPQSSRCNSWRLGTDEPLSLEGAQ
                     VTSPALTELRANPTARAALWQQICTYEHDFFPRND"
     gene            20291..21322
                     /gene="rsmC"
                     /locus_tag="J1Y83_002473"
     CDS             20291..21322
                     /gene="rsmC"
                     /locus_tag="J1Y83_002473"
                     /EC_number="2.1.1.172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(1207)-N(2))-methyltransferase
                     RsmC"
                     /protein_id="HBA7630617.1"
                     /translation="MSAFTPASEVLLRHSDDFEQSRILFAGDLQDDLPARLDTAASRA
                     HTQQFHHWQVLSRQMGDNARFSLVATADDVADCDTLIYYWPKNKPEAQFQLMNLLSLL
                     PVGTDIFVVGENRSGVRSAEQMLADYAPLNKVDSARRCGLYFGRLEKQPVFDANKFWG
                     EYSVDGLTVKTLPGVFSRDGLDVGSQLLLSTLTPHTKGKVLDVGCGAGVLSVAFARHS
                     PKIRLTLCDVSAPAVEASRATLATNGVEGEVFASNVFSEVKGRFDMIISNPPFHDGMQ
                     TSLDAAQTLIRGAVRHLNSGGELRIVANAFLPYPDVLDETFGFHEVIAQTGRFKVYRA
                     IMTRQAKKG"
     gene            21522..21608
                     /locus_tag="J1Y83_002474"
     tRNA            21522..21608
                     /locus_tag="J1Y83_002474"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:21556..21558,aa:Leu,seq:cag)
BASE COUNT         5040 a         5923 c         5547 g         5130 t
ORIGIN      
        1 agcatcgcat ccggtattag ttaaatgatt aatgggaggc aagttcccgg aaaaaggcag
       61 gattttcttt aattgtggca agtacttttg cagccaatcc cgtaggattt cttcgcttcg
      121 attcccagct tttaatggta tccacactgg ttcccagagc ttttgccatc tcactttgag
      181 aaacgttgag ttgctcacga atagctttaa catccgcaat ttcgtagcgg gtcacccgtg
      241 caggttcttt taaaccttgt ttaatctcga cagcctcttc cagagaggtt ttcaactcat
      301 caaaaaaact cattttatat ctcgcctttg agcagtttcg tcaacttctt tagttgagct
      361 ttttccgtat ctgtcagact atctttggca tttttcggat aggccataat caaataaata
      421 atctcttccg tcgccagaga gtaaattatt ctgacactgc cactttttcc ctgtgaaccc
      481 gcagccattc ggactttacg caatccacca gtctgctgaa tgagatcacc tttatcggga
      541 gttccgatga gttctttttg taattccttt aattcatcat cggttgcaat ctgcttgatc
      601 tgacgcgtaa acattggcgt ctctataaat attatcgcct tacccattac ccctccctgg
      661 caggtacatt gtacaccaaa atggagtact aagtacactt ttgaatcagt aatcccactc
      721 aacatcttat aaagatgatt atctttgctc ccccatcatc tcccgcacca gctccacgca
      781 gcgcaggaaa cggctgtcgt aatcgtcctc ttcaacccgc acgaattcga tattgttctc
      841 ttccagcatc tccaccagca agttctggaa ctctttgcga tccaccgaac tgccgaggct
      901 gcgtaaacca tccgccaccc acggcgtgtt gttctccagc aggatcacca gatcaaaacg
      961 gtattcatca atcaacgcct gcacgaacgg atgctcacgc ccttcgtact ttttgcagaa
     1021 cgcctgggta gtgacaaaat cggtgtcgat aaacgccact ttattggcat atttcactgc
     1081 aaaatcaata tattgcgcat gtcccagcgc gattttatcg tagtcggaat actgcaacgc
     1141 gatctcatcg ccaccgaggt gcgaaaagac ataatcacga ccatattccc acgcactggt
     1201 agtgttgaag atattggcaa gtttgtttac caaggtggat ttaccgctcg attcaccacc
     1261 gaggatcgcc acggtacgca caaagaatgg cttcacttcc gtaggaatat attcccagta
     1321 gcggaacggg ttttcgcgga tctgcgcacc gctgatactc ataaaggtac gcttcggatc
     1381 gatcaacacc gtctcgatcc ccagatgttc catatactgt ggcgcatcgg cttcttccga
     1441 ggtgtagatc aggtccggct gaatcccttt ttcagccata aactttttga tgccgttgct
     1501 ccacacatcc cagccgtgcg gatatggctc catgccctct tcgttgaagg catgaatacg
     1561 gatatttttc tgatatttaa aggtttgcaa tagccagcgc agacgatccg gcaccgtggg
     1621 ctgctgcgac atggcgctgt cttcgaacaa tgcgcggtcg cgggtatcgt caaaacccat
     1681 aatgatatgc agctcgtcaa cctggctaca ggcgcgctgg ataaggtaga tatgtccagt
     1741 atgcagcggg taaaacttac cgaatacgac accgatcgtt ttcttctgcc ggggaaattc
     1801 aagtcccaaa taacggtgca atgcctccag cttttgtgcg ctggggcttt tgattttggc
     1861 attcagtaac tggcttaaat accctttggt cataccgctg gcatcagcta cctgctgtag
     1921 cgtgcagccc tgttgcttga tggcagtttt caggtaatca aatgacgaca tatctccctc
     1981 cgtatctctc attataagtc gtcgaacacg ctaagcgcgt cggagagttt tttaacgcca
     2041 aaaatctgca tcccttccgg cgcttttttc ggcacgttag ctgccggaac aattgcccgg
     2101 cgaaaaccgt gtttcgccgc ttctgagatt cgttcctggc cgctgggcac cgggcggatc
     2161 tcccctgcca gcccgacttc accaaacacc accagatcct gcggtagcgg tctgtcacgc
     2221 aggctggaaa ccatcgccag cagtaacgct aaatcggcac tggtttccgt caccttcacg
     2281 ccaccgacca cgttaacaaa cacatcctga tcggccattt gtagaccacc gtgacggtga
     2341 agcaccgcca gcaggattgc cagacggttt tgctccagcc cgactgccac gcggcgtgga
     2401 ttcgccatca tcgagtgatc gacgagcgcc tgaatctcca ccagcagcgg acgcgttcct
     2461 tcccacacta ccatcactga gctaccggag gtcacttcat cgccgcgact taagaaaatt
     2521 gccgaagggt tgctgacttc acgcagcccc tgctcggtca tggcgaagac acccagctca
     2581 ttcaccgcgc cgaagcggtt tttatggctg cgtaaggtgc gaaaacgtga gtcggcatcg
     2641 ccatccaaaa gcaccgaaca gtcgatacag tgttccagca ctttagggcc agccagcgag
     2701 ccatctttgg ttacgtgccc gaccatgaca atcgccacac cgcgcgtttt ggcgaagcgc
     2761 gtcagataag ccgccgtttc acgcacctgt gccacgctgc caggcgatga ttgtacatcc
     2821 gccatatgca tcacctggat ggagtcaatt accatcagct tcggttgctc ttcttcggca
     2881 atcaggcaga tctgctcgat gctggtttcc gacagcatat taagattgtc agtcggcagg
     2941 ccaaggcgat gagcgcgcat tgccacctgt tgcagcgact cttcgccggt gacatacagc
     3001 gttttcatct gctgcgccag tttgcacagc gtttgcagca acagcgtgga tttccccgca
     3061 ccagggttac cgccaatcag aatggcgctt cccggcacca cgccgccgcc taatacgcgg
     3121 tcgaactctt taaatccggt ggaaaaacgc ggcagctctt caaggctgat gtcggaaagt
     3181 ttctggactt ttgccactcc ggcgctaccg gcatagccac tgagacgctc gttacgcgcc
     3241 accgttggcg acgctgcaag acgcacctcg gtgatggtgt tccaggcatg acaggcactg
     3301 cactgcccct gccagcgcgg ataatcggcc ccgcattcat tacaaacaaa ggcgcgtttt
     3361 ggagcttttg ccacgatgta cctcgttaat gctgtgccgc ccgcaggatg acgggcggac
     3421 aattacttct gattcaggct gccagagagg atgcagaata cccccatcag gtcagcgtga
     3481 cggatggtga cttccgtctt ttcattcact tttggcttgg catggtaggc aatccccagc
     3541 cctgccgctt tgatcatcgg caggtcattg gctccatcgc caatcgccac ggtctgcgcc
     3601 agcgggattt catactcctg cgcgaggcgg gtcagggttt tcgctttata ttgcgcgtca
     3661 acgatgtcgc cgatcacatt gccggtaaat ttaccgtcca tgatctccag ttcattggct
     3721 accacggctg tcaggcgcag cttgtcgcgc aggtattcag caaagaaagt aaagccgccg
     3781 gaggcaatcg ccactttcca gcccagcgtt tccagcttga gtaccagctg cgttaagcct
     3841 ggcatcagcg gcagattttc acgcacctgt tgcagaatat tggcgtcagc gcctttcagc
     3901 gtcgccacgc ggctgcgcag gctggcggta aaatcgagtt cgccgcgcat cgcccgttcg
     3961 gttacctccg ccaccctctc gcccgttccg gccagtttgg caatttcatc aatacattca
     4021 atctggatgg cggtggaatc catgtccatc accagcaacc ccggcgtgcg caggtgtggg
     4081 attttcccca gcggcgcgac atccagttgc gcttcatggg ccaggcgcgt agcccgcgca
     4141 gtgagtgaac ctgccagacg aatcacctga taatcttcca cgcaccaggc ggcaacaatc
     4201 accatagccg cgcccagttt gctctggtat tgggtcagac gttgtttatc cagcccacga
     4261 ccatacagca gccagccgct acgacctgcg tggtaatcca gtggcatcac ttcatcacca
     4321 cttaatgaaa gaggcagacc aggccataaa gagacatctt caggcaggtc gcaccaggta
     4381 atgttaggca ttaaggctcc tgtaaaatcg ttcgaagcag ggaaaataac gcatgaggct
     4441 accttgtatc cattgcttct ggcaacatta agtctcaaat tttcaaaggg tggaagatgg
     4501 ctcgcacaaa actgaaattc cggctgcatc gggcagtgat tgtcctgttc tgtcttgcct
     4561 tgttagtggc gctgatgcag ggggcgtcat ggtttagtca aaaccaccag cgacagcgta
     4621 atccgcaact ggaagaactg gcccgcaccc tggcgcggca ggtgacgcta aacgttgcac
     4681 cgctgatgcg taccgactca ccggatgaaa aacgcattca ggcgatcctc gatcagttaa
     4741 cggatgaaag ccgcattctc gacgcaggcg tatatgatga gcaaggcgat cttatcgccc
     4801 gttctggcga aagcgtcgac gtgcgcgacc ggctggcgct cgacggcaaa aaagcgggcg
     4861 gctattttaa ccagcagatt gtcgaaccca ttgcgggtaa aaacggaccg ctcggctatc
     4921 tgcgcctgac gctcgacacc catacgctcg ccaccgaagc ccaacaggtg gataacacca
     4981 ctaacatttt acgcctgatg ttgctgctct cgctggcaat cggcgtagtg ctgacccgca
     5041 cgctgctaca gggtaaacgc acccgctggc agcaatcgcc cttcctgtta accgccagta
     5101 aaccggtgcc ggaagaggaa gaaagcgaga aaaaagagtg acccattact acaagaaagg
     5161 aaatcgttat gtccacatta cgcctgctca tctctgactc ttacgacccg tggtttaacc
     5221 tggcggtaga ggagtgtatt tttcgccaga tgccggctac gcagcgcgtt ctgtttctct
     5281 ggcgcaatgc cgacacggta gtgattggtc gcgcgcagaa cccgtggaaa gagtgtaata
     5341 cccggcggat ggaagaagat aacgtccgcc tggcgcggcg cagtagcggt ggcggcgcag
     5401 tgttccacga tctcggcaat acctgtttta cctttatggc tggcaagccg gagtacgata
     5461 aaaccatctc cacgtcgatt gtgctcaatg cgctgaacgc gctcggcgtc agcgccgaag
     5521 cttccgggcg taacgatctg gtggtgaaaa ccgccgaagg cgatcgcaaa gtctcaggct
     5581 cggcctatcg cgaaaccaaa gatcgtggct tccaccacgg cactttgctg ctcaattccg
     5641 accttagccg cctggcaaac tatctcaatc cggataaaaa gaaactggcg gcgaaaggca
     5701 ttacctcagt gcgttcccgc gtgaccaatc tgaccgaatt gctaccgggg atcacccatg
     5761 agcaggtttg cgaggccata accgaggcct ttttcgccca ttatggcgag cgcgtggaag
     5821 cggaaatcat ctccccggac aaaacgccag acttgccaaa cttcgccgaa acctttgccc
     5881 gccagagtag ctgggaatgg aacttcggtc aggctccggc attctcgcat ctgctggatg
     5941 aacgctttac ctggggcggc gtggaactgc atttcgacgt tgaaaaaggc catatcaccc
     6001 gcgcccaggt gtttaccgac agtctcaacc ccgcgccgct ggaagccctc gccgggcgac
     6061 tgcaaggctg tctgtaccgc gcggatatgc tgcaacagga gtgcgaagcg ctgctggttg
     6121 acttcccgga acaggaaaaa gagctacggg agttgtcagc gtggatggca ggggcggtaa
     6181 ggtaactcgc tgattaaaat atccagggaa ggaaatatga tcgatctgga aaaccaggaa
     6241 cgggagatta tcaatttaat gttcagccag ggtatatcct ggctaaccgc cgtgcgtatc
     6301 aggcataaac tatcacttgc tgaagtaagt aaaatgttag gtatctccat taattctcta
     6361 aaacagatcg aaaagacgga acggcttagt tcaaacatca aaagcaaaat ggcaggaatt
     6421 tatggttgtt caccagagtt gctgatatgc ccttcctgga tgactgctga acataaatga
     6481 atagattcgt gaatagatac ttgaatggat tttattttct atccatatga aaaataatag
     6541 taaatctata aatcagttgc tattgatgaa taaatactaa aaagccgggg cagtctccca
     6601 ctccggcttc atacgacaat cgcttttcag caaaacacaa ttactcttta tcgcccagca
     6661 gaacggattc cagcgcgatt ttgatcatgt cgttgaaggt ggtctgacgc tcagcggcag
     6721 tggtctgctc gtgagtgcgg atgtggtcag acacggtgca gatggtcagg gctttcgcgc
     6781 caaattctgc agcgacgccg tagataccag ccgcttccat ttccacgccg aggatgccgt
     6841 atttttccat cacgtcgaac atttcgccgt ccggagagta gaacaggtca gcggagaaca
     6901 ggttaccaac acgagcatca acgcccagtg cttttgccgc gtctaccgcg ttacgcacca
     6961 tgtcgaagtc agcgatagcg gcaaagtcat ggtctttaaa acggatgcgg ttaactttgg
     7021 aatcggtgca ggcacccata ccgataacga cgtcgcgcag ttttacgtgc ggcagaacag
     7081 cgccacagga accaacgcgg ataattttct tcacgccgaa atcagtgatc agttctttgg
     7141 tgtagatgga gcaggacggg atacccatac cgtggcccat tacggaaatt ttgcggcctt
     7201 tgtaagtacc agtgaagccc agcataccgc gaacgttgtt cacttcacgg gcatcttcaa
     7261 ggaaagtttc agcaatatac ttcgcacgca gcgggtcgcc tggcatcaaa actacgtcag
     7321 caaaatcgcc catttctgca ttaatgtgtg gagtagccat cattttatcc tttttgtgac
     7381 atattaaagg cggagtcact gctccgccca aatccatcag aacatggctt tgccatattc
     7441 catatcagaa gtaccaaaat attttgccag agtctggccg atatccgcga aggtttcacg
     7501 atgccccagc gaacctggtt tcactttcgg gccgtaaacc agtaccggaa tgtgttcacg
     7561 cgtgtggtca gtaccggtcc aggtcgggtc acaaccgtgg tcagcagtga gaatcagaat
     7621 atcgtcatcg cgcagcaaag acatcagctc cggcagacgg cggtcgaaca gttccagacc
     7681 cgcagcataa ccggcgacgt cgcgacggtg gccccaggaa gagtcgaagt caacgaagtt
     7741 ggtgaagacg atggtgttat cacccgcttc tttcatctct ttgatggtgg cgtcaaacag
     7801 cgcgtccagg ccggtcgctt tcactttttt ggtgataccg cagttggcgt agatgtctgc
     7861 aattttaccg accgaaacca cctggccgtg tttttcatca accagtttct gcagcacggt
     7921 cggtgctggc ggctcaacag ccaggtcgtg acggttacca gtacgctgga agttaccagc
     7981 tttgtcgccg ataaacggac gagcgataac acgaccgatg ttgtagccgc cgttggtcag
     8041 ctcttcacgg gcgatttcgc acagttcgta gagtttatcc agaccaaagg tttcttcgtg
     8101 gcaggcaatc tggaacacgg agtcagcgga ggtatagaaa atcggcttgc cggttttcat
     8161 gtgctcttcg cccagttgat ccagaatgac cgtaccggaa gagtggcagt taccgaggta
     8221 acccggcaga ttagcgcgtt cgaccagttt atccagcaac tcttgcggga agctgttttc
     8281 gtgatcggag aagtatcccc actcaaacag aaccggcaca ccagcaattt cccagtgacc
     8341 agacggggtg tctttaccgg atgacatttc gtgcgcccat gcgtacgcgc cgataacttc
     8401 agcgttgcca tccattcccg ccggaatgaa accggtagac ccttcgtgtg ctttcgccag
     8461 cccaagacgg gtcagatttg gcagattgag cgggccttta cgaccgtggt cagcttcgcc
     8521 tttggcacaa gcttctgcga tatgacccag ggtgtcagcc ccgacgtcac caaagcgttc
     8581 tgcatcttct gtagcgccga tgccgaatga gtccagcacc ataataaatg cacgtttcat
     8641 atgttctccg tactcagtgc ttcaaattta aatgatcaga tcagtatacc gttattcgct
     8701 gatacggcga tagacagttg gtgtgctttc cggtgcttta tcggcaagtt taattgccgc
     8761 tttcaccgct ttcgccgctt cctgccagct gttttcgtct ttcgcgtgga taactgccag
     8821 cggacgctga ccgtctacct ggtcgcccag acgcgccata tcagtaaagc cgacactgta
     8881 atcgatggtg tcagatgcct gacggcgtcc gccgcccatt gcaaccactg ccatccccag
     8941 cgcgcgggta tccatttcac tgacaaaccc ttcggtatca gcatagactg ctttcgtcag
     9001 catcgctgtc ggcagatact tcgcgtagtt ctcaacgaag tcggtcgggc ctttttgtgc
     9061 cgctaccata cgaccaaaga cttctgccgc tttaccgttg tccagcaccg cctgtaattt
     9121 cgcgcgcgct tcggcgtcat ctttcgccag tttgccggag atcagcatct ccacgcacag
     9181 tgccatcgtg acatcaaaca gacgcgggtt acgatattca cccgtcagga actgcaccgc
     9241 ttcacgaact tcaaccgcgt tacctgcact ggaggccagt acctgattca tatcggtgag
     9301 cagcgcggtg gtgcgcacgc cagcgccgtt agccacgcca acaatcgctt cggcaagggc
     9361 ttcagagagt tcgtaggtcg gcataaacgc gccgctaccc actttcacgt ccatcaccag
     9421 cgcgtccaga ccttccgcca gtttcttcgc cagaatagag gcggtgatca gcgggattga
     9481 atccacggtt gcggtaatat cgcgggtcgc gtagaaacgt ttatccgccg gagccagtga
     9541 gctggtctgg ccgataatcg ccacgccgac gtctttaata atttcgcgga aacggttgtc
     9601 atccgggaaa atgtcgaagc cagggatgga ttccagtttg tcgagcgtac cgccagtatg
     9661 accgaggccg cgaccggaga tcatcggaat atagccgcca caggcagcga ccatcggccc
     9721 caacatcagc gaagtcacat cgccgacacc gccggtcgag tgtttatcaa caatcgggcc
     9781 attcagatgc aggcttttcc agtcgagaac ggttcctgaa tctcgcatcg ccatggtcag
     9841 cgagacacgc tcaggcattg tcatatcgtg gaagaaaatg gtcatcgcga gggcggcaat
     9901 ctgcccttcg gagatagtgt tgtcgcgaat gccattgata aagaaacgaa tttcatcatc
     9961 actcagcgca tgaccatcac gttttttacg aataatttct tgtgcgagaa acaaggtaac
    10021 ctcccgtgga aaagagacga ggtaatcggg gaaccggatg agcatcgctg catcatccgg
    10081 caaatcaagc cttagtagct gctggcgctc ttaccgtcgc cgtggcccag agctttcagc
    10141 aggcttgcca gcaagctgga agcaccaaag cggtagtgac gcgcatcagc ccagtcagca
    10201 ccgaacagtt catctgcaat ggcgagatat ttctgcgcat cttccgcagt acgcacgccg
    10261 cccgccggtt tgaaaccaac ggttttttct acgcccatat cgcggatcac ttccatcatg
    10321 atgcgcgcgc tttccggcgt cgcattcaca gccactttac cggtagaggt tttgatgaag
    10381 tcagcacccg ctttgatgga gatttcagac gctttacgga tcagcgcttc gtctttcagt
    10441 tcgccggttt caataatcac tttcagcagt acgttcgccg cagcgcaagc ctctttacag
    10501 gctttcacca ggtcaaaacc aacctgctcg ttacctgcca tcagcgcacg gtacgggaac
    10561 actacgtcaa cttcatcggc accgtaggcg attgccgcac gggtttctgc cagcgcgatt
    10621 tcgatgtcat cgttaccgtg tgggaagtta gttaccgtag cgatacggat ttccggggtg
    10681 ccctgctctt tcagcgtttt gcgagcaatt gggataaagc gaggatagat acagatagcg
    10741 gcggtattgc cgaccggagt tttggcctga tgacacaggg cgatcacttt ctcgtcggtg
    10801 tcgtcgtcat tcagggtggt caggtccatc aatttcagtg cacgcaggct gcttgctttc
    10861 agatcagtca tttcattctc ctggcttgtc gccaataaaa ttcaccttgc gagtttgtta
    10921 gtattctaac atctactccg caacacactt cgatgtacat cacaattaag gaaatctact
    10981 tacaagtttg catcactgta atgcgatctg gttcaaataa ttcactttca aatgaatgcg
    11041 tcagtggtgg caaacgtatc aggatcaaag tgaacatcac gaaacttctt acaatggcgc
    11101 atcaccttcg gcataaaagg aaaagaagat gcccacttct catgaaaatg cactgcaaca
    11161 acgttgccag caaattgtca ccagcccggt acttagcccg gagcagaagc gccattttct
    11221 ggcactggaa gcagaaaaca atctgcctta ccccgcgttg cctgccgaag cccgccgcgc
    11281 gctggatgag ggggtaatct gcgatatgtt tgaaggtcat gcgccgtaca aaccgcgcta
    11341 tgtcttaccc gattacgccc gttttctggc gcacggttcc gaatggctgg agctggaagg
    11401 cgcgaaagac cttgatgacg cactctctct gctgaccatt ctttaccacc atgtaccgtc
    11461 ggtcacatcg atgccggtct acctggggca actggatgcg ttgttgcaac cgtatgttag
    11521 aattctaaca caagacgaga tcgatattcg aataaaacgt ttctggcgtt acctcgacag
    11581 aaccctgcca gacgccttta tgcatgccaa tatcggcccg tctgattcgc ccattacccg
    11641 tgcgatatta cgtgcagacg cagagctgaa gcaggtttca ccgaacctga cctttatcta
    11701 cgatcctgat atcacccctg atgacctgct gctggaagtg gcgaaaaaca tctgtgaatg
    11761 tagcaaaccg cacatcgcca acggtccggt gcatgataaa attttcacaa aggggggcta
    11821 cgggattgtg agctgttaca actcactgcc gctggcgggt ggtggcagca cgctggtacg
    11881 cctaaacctg aaagccattg ccgagcgcag tgaatcgctg gaggacttct ttacgcgcac
    11941 tctaccgcac tactgccaac agcagatcgc catcatcgat gcgcggtgtg aattcctcta
    12001 tcaacaatca cacttctttg agaatagctt cctggtgaaa gaagggctga ttaaccctga
    12061 acgttttgtg ccgatgtttg gcatgtatgg gctggcggaa gcggttaact tgctgtgtga
    12121 aaaagaaggg attgccgcac gctacggtaa agaggccgcc gcaaatgaag tgggttatcg
    12181 cattagcgcg caactggcgg agtttgtcgc caatgccccc gtgaaatatg gctggcaaaa
    12241 acgcgccatg ttacacgcac agtcggggat aagttccgat atcggcacca cgccgggcgc
    12301 gcgtttgccg tatggtgatg agccagatcc gattacccat ctgcaaactg tcgccccgca
    12361 tcatgcttat tattattccg gcatcagcga cattctgacg ctcgacgaaa ccatcaaacg
    12421 taatccgcag gcgctggtac agctttgcct cggtgccttt aaagccggaa tgcgtgaatt
    12481 taccgccaat gtcagcggta acgatctggt tcgcgttacc ggttatatgg tgcgtttgtc
    12541 ggatttagaa aaatatcgcg ccgaaggttc acgcaccaac actacctggc tgggagaaga
    12601 agccgcacgc aacactcgta ttctggaacg ccagccgcgc gtgataagcc atgaacagca
    12661 gatgcgcttt agtcagtaag attatcccct tctcctgcgt tgacgggcca ggcagtcgtc
    12721 tggctctgtt tttgcagggc tgcaatctgc gctgcaaaaa ctgtcacaat ccgtggacga
    12781 tggggcgctg taatgattgt ggagagtgcg tgccgcagtg tccgcatcag gcgttgcaga
    12841 ttgtcgacgg caaagtggcg tggagcgcgg ctgtctgcga gcaatgtgat acctgcctga
    12901 agatgtgtcc gcaacatgct acgccaatgg cgcaatccat gagcgtggac gaggtgctta
    12961 accatgtccg caaagcggtt ctgtttattg aagggataac ggtaagcggc ggcgaagcca
    13021 cgacccagtt gccgtttgtg gtggcgctgt ttactgccat caaaaacgat ccgcaactgt
    13081 gccatctcac ctgcctcgtg gacagtaacg gcatgttgag cgaaaccggc tgggaaaaat
    13141 tgctcccggt gtgtgacggc gcaatgctcg atctcaaagc gtgggggagc gaatgtcatc
    13201 agcaattaac cggacgcgat aatcagcaga tcaagcgcag catctgtttg ctggcagagc
    13261 gcggtaagct ggcggaactg cgtttgctgg taatccctgg ccaggtggat tatttgcaac
    13321 atatcgaaga actggcggcg ttaatcaagg gacttggcga tgttccggta cgcctgaacg
    13381 cgtttcatgc ccacggcgtg tatggcgagg cgcaaagctg gccgagcgcc acgccggagg
    13441 atgtggaaca actggctgat gcgttacggg agcgcggggt gagccggtta atatttccgg
    13501 cgctatattt gtgacatgcc ggatgtggcc tacggatcgg cacagacctg taggcatgat
    13561 aagacgcgtc aagcgtcgca tcaggcattg tgcgccagct gccggatgcg gcgtgaacgc
    13621 cttatccggc ctacggttcg gcacagactt gtaggcatga taagacgcgt cagcgtcgca
    13681 tcaggcattg tgcgccaacg gccggatgcg gcgtaaacgc cttgtccggc ctacggcacg
    13741 ttaaacaacg tataggtgtt attaagcaac acttcggcaa tctcatccgc cggttccggt
    13801 cgtaactcgc aaagcacggc aaacacacgg gcggcctgtt cagggcgatt aggctgcccc
    13861 tgaaaaccat tgagcggcat atctggtgcg tcggtttcca gcaacaacga tgccagcggt
    13921 aattttgcga tgacatcgcg ggttttactg gcgcgtggat aggtgatagt cccgcctacg
    13981 ccaattttgt agcccagttg cacaaaccgt tcggcctgtt gcaggctgcc ggaaaaacca
    14041 tgaaccacgc cagtgcacgg taaatcgtgg cgtttaagat gcatcgccag tttgtcgtgc
    14101 gtgcgtcgtg aatgcaggat caccggcaga tcgtagcgtt tcgccagttt cagttgttcg
    14161 tcgagtaacc actgctgcct ctcaaattgc ggatcgtcgc caaagagatc cagaccgatc
    14221 tcccccaccg ccaccacctt cgccggatgc ctttccagcg cctgctgtag ctgatcaaga
    14281 gacacatcgc tatgtttttc caacatacca ggatgcaagc ccaatgcggc atacagcggt
    14341 tgatattttt ccgctaacgc ctgcacgcgg gcaaaatttt ccgcctctgt cgccggaaca
    14401 atgatcttgc ctacgcccgc ttgtgccgcg cgttgcaggc tggcctcttc atcgccacta
    14461 aacggcggga aatcaaaatg gcagtgggtg tcgataaaac ggcaaatcaa gccagatcct
    14521 cattgttaaa tgtcgggtcg ttcgcctgcg gagcgtcgct cacttcggca accagcgtat
    14581 cgttggcgac tggcgcaggc ggaataacga tcgactccgg cgtcaccacc ggcaccagat
    14641 ggcggctcag cggcgctttt tcagtcaata atttaccgac cgttgccaga aaataacgcc
    14701 cacataaacg cccaagttta tagtcttcac gcagcgccgg aatccgactg ccgagcgcaa
    14761 tactatgcaa tggcttcggc ggataaattt cgaatatccg cagctggccc ggtggtttct
    14821 caataaattg ctgaatctca cgatagctgg tttcatgatg ctgcaccaga ttgaccagcg
    14881 gctgcaggct actgtcaccc agccagcgtt ccatgcgttt gaaccactgt ggcgtgtagt
    14941 acatttgtga cggcacagtg cggatgacga ccaacgtttt tgctccctgc cttgccgcct
    15001 ctttaaccgg gatcgcatca ctgatcccac catccaggta gttaatacct tccagcgaca
    15061 ctccgctacg ataaaagcca ggtatcgcac tggaggcgcg aatcacatcc agccagtttt
    15121 gtttagttgg caaaaagtaa ttcggcgcat agtcatcctg acgacaggcg cacatataaa
    15181 acgatttgcc gctgtcaaat aaccgcgctg cggtatccat ttgcaacggc atctggcttg
    15241 cggtggcctc caccagccag tcgagatcga taagatttcc tccacggaca aagcgcaatg
    15301 gatcgaaaaa ttcgcgtttt gtggtgtaac gcatgatgac tttgcgcgcg taaccgggct
    15361 gattgcagat atacgccgag aggttctgcg ccccggcaga tgtgccgaga taaagatcga
    15421 aaggattaaa ctgcgcgcgc ataaactcat ccagcacgcc agccgtgaaa attccacgtt
    15481 gtccgccgcc ttcgcacacc agagcaatcc gtccaggctg gaacggcctt agcgacaacg
    15541 gcgcaatatt accaagcgtt acaggtattc gctgccccac ctctgccttc ctgtttttgt
    15601 tgtgaaagaa tgacaaagta acgcaattta ttattcgcta aaaccgcaaa agccagtccg
    15661 ctggactggc tttggataaa attttcttaa tggtctgaaa tctagggtcg ttttcggccc
    15721 atgaacaaac tcaccaggaa cagaataatc ccgacgacaa agacaatttt agctgcgcct
    15781 gcagcagtac cggccagacc accaaaccca agtgcggcgg cgattaacgc gataaccaga
    15841 aatatgatgc cccaacgaaa cataagcgtc tcctttacca tagttaatgt caccgcttga
    15901 tatgagcgtg aaaatcactc aatgcgatat ttttagtgtg gtgcacatcg cgcctcccga
    15961 caaagttcgg gaggtcgaat tacgacgaat tacttagttt tcagatcatt tttaacgctt
    16021 ttcacaccat ctaccgcttt ggcgatactt tcagcacggt cgctctgtgc ctgagaatct
    16081 acggtaccgg agagctggac cacgccatcg gtggtttcaa ctttcacatg acgggaaggg
    16141 acgatatcgt ccgccagcag tttggctttg atttcactgg tagtggcggt gtcacccgcg
    16201 tagcctttca cagagccttc tttagcgtcg cgaacgtgca gtttgtcgct gacagaggtc
    16261 acgccctcaa cacctttcgc cactttcacc gcctcttcgg cctgagcctg gctttcaacg
    16321 aaaccgctca gggtcacgac tttttgatcg gttttgacag agatatcggt gctcttgatg
    16381 ttgtcatggt ccaccagggc cgccttcact ttcgcggtga tggcgctgtc atccatgaaa
    16441 ttaccgactt tattcataga gctatcgact ttttgccctg cgctttcatt ggtagtctgc
    16501 gcgttgtttt ctgcgtaggc agagccggtc gcgacggcag aggtcaacat tacagccagc
    16561 agagttttcg aaatcttcag tcttgtcata gtcatcgatt tattcctgta tgtttgctcg
    16621 taatttgagc ctggcaacac gaggttgcat tgctgaatag ggagagactt caccctctac
    16681 agaagtcaat ggtcgccatc acaaaagcga tgggtgatga ataacgacca ttacagcctc
    16741 tgaatcagtt attaatatcg gtagaatgac aatcgacggc tttagatact gatatctacg
    16801 cattgaacgg tatttaacgc cgtcataaat gtcattactt tgttaaatat agatcacaat
    16861 tttgaaaccg ctcgggatat cagctaaaac ataagcaaaa gtgaatgttt taagaacatt
    16921 ccgtaagcgg ctaataagga agggaaattg acagggcgca gcggctaccg cgccctagag
    16981 gctagaatta atgctcgcgg gtctggtgga actgaacgtc cggataacgt tcctgcgcca
    17041 ggcgcagatt gaccatgctg gtagcgatgt aagcgaggtt atcgccgcca tcaagcgcca
    17101 gttggctttc gttcttacgc ttgaactctt cgaatttctt agcgtccgca cattctaccc
    17161 agcgggcggt ggcaacgttg actgactcat acactgcttc aacgttgtat tcgctcttca
    17221 ggcgcgctac caccacatca aactgcagca caccaaccgc gccaacgatc agatcgttgt
    17281 tggagatcgg acggaacacc tgcaccgccc cctcttcgga aagctgtacc agccctttga
    17341 gaagctgttt ttgcttcagc ggatctttca ggcggatacg acggaacagt tctggcgcga
    17401 agttcggaat accagtgaac ttcatcatct caccctgggt aaaggtatcg ccgatctgaa
    17461 tggtgccgtg gttgtgcagg ccgaggatat cgcccggata cgcttcttca acgtgcgaac
    17521 ggtcgcctgc cataaaggtc agcgcgtcgg agatcaccac atctttcgcg gtgcgcacct
    17581 ggcgcagctt catgcctttt tcatatttac cggataccac gcgcataaac gccacgcggt
    17641 cgcggtgttt cgggtccatg ttggcctgaa ttttaaatac gaagccggta aatttatctt
    17701 cgctcgcttc tacggtacgg gtatcggtct gacgcggcat cggcgctggc gcccactcca
    17761 ccaggccatc cagcatatga tcgacgccga agttacccag cgcagtacca aagaataccg
    17821 gggtgatttc gcccgcaagg aacagctctt tgtcgaactc attagacgcg cctttcacca
    17881 gctccagttc gtcacgcagc tgctgtgcca gatcttcacc aaccgcagcg tcgagatccg
    17941 ggttattcag ccctttaaca atgcggactt cctgaatggt gtggccttta ccgctctgat
    18001 agagataggt ttcatcttta taaaggtggt aaacgccttt aaacagcttg ccgcagccaa
    18061 tcggccaggt gatcggtgcg cagccgattt tcagctcgtt ctcaacttca tcgagcagtt
    18121 ccatcgggtc gcggatatca cggtcaagtt tgttcataaa ggtgaggatc ggcgtatcgc
    18181 gcagacgggt aacttccatc agcttacggg tacgatcttc tacacctttt gcggcgtcga
    18241 taaccatcag gcagcagtcc accgccgtca gggtacgata ggtatcttcc gagaagtctt
    18301 cgtgccccgg ggtatcgagc aagttaacca ggcagtcgtg atacggaaac tgcatcacag
    18361 acgtagtgat ggagatccca cgctgctttt ccatctccat ccagtccgac ttagcgtgct
    18421 ggttagagcc acggcctttt actgtaccgg cggtctgaat ggcctgtccg aacagcagca
    18481 ccttctcggt gatggtagtc ttaccggcgt ccgggtgaga aataatggca aaagtgcggc
    18541 gcttcgccac ctcttgcaaa taaggagaca acgtcataat tctttcttct tgagtaagcg
    18601 gcatcgcgcc gcgcttgttg aaaacgaaaa attgcggcta ttttacccat caacgggggg
    18661 gaggcaatca gtgtttacac aggagctgct ccagttcgtg caacgaagaa acggtccagg
    18721 tgggcgcgat gccttctggt tgctcgcgat tgtgcgcatt cagccagcag gtcgcaagcc
    18781 cggcgttgat gccaccgaga atatcggact cggcagtgtc accaaccatc agcacgcgtg
    18841 aacggtcagg attgcccgct tgttccagcg cataatcgaa aattttctta ttcggtttgg
    18901 caacgccaac ttcttcggaa atcaccagca aatcgaagta atcacgcagg cccgtgcgtt
    18961 ccagacgtac ctgttgcaag gcgctaaagc cgttggtgat gatgccgatt ttggcgttgc
    19021 cacgaatggc gttaagcagg gaaaccgccc ccggcaacgg cgtacagatt tccgccatcg
    19081 cattaataaa ggcctcgttg agtttacccg gttcgacgtt cagccgttcg gcccagctct
    19141 caaaccgccc gtgctgaagc tgtaatgaag tgatcacgcc gttttgataa tccacccaca
    19201 gtggcttgtt aacggcctga tagtcctgaa aatcttcagc ggtaaaggtg acgctgtaat
    19261 caagaaacat ccgctgcagg ccggtgaatg agtcaaaggt aaacagcgtt tcatcggcat
    19321 caaagaaaat ccagtcccac ttcattattc caccttgtct tacatactga ttggtaacgc
    19381 catgatgatg gcgtcttcgc gaccgtccgt ggtggggtag taattgcggc gaatcgtcgc
    19441 ctcgttaaag cctaaacttt cgtacagggc aatggcggca gcatttgagg cacggacttc
    19501 cagccatagc gtcgccacgc cgcgtttttc cagttcgtcg atcagatgtt ccagcagcac
    19561 ccttcccagt ccctgacgct gatagtcagg atcgaccgca atattgaaca atgtagcttc
    19621 atccagcacc acttgcgtaa tcgcaaacgc cgccattttg ccgttttgcg ttaactgaaa
    19681 gttgagatag cgctcgccct gattactggc aaaggttttt tcactccacg gaaaggtgtg
    19741 ggcgcgttgt tcaatgtgga aagccgctgg taaatcagtc gtttcgaggg aagaaatcgt
    19801 gttcatatgt gcaaatttgt tgccataacg cggcgcgtgc cgttgggttt gcccgtaatt
    19861 cggtgagcgc cggtgatgtc acctgagcgc cttccagtga tagcggttcg tcagtaccca
    19921 accgccagct gttgcagcgg ctgctttgcg gcagcatcgc gattttttct ggcgtcagtt
    19981 gcagcacttg atcggggctg acggttaacg cacgcagaac atcgctcacc agaggatcgg
    20041 tcagggcggg aagatcgttt gccaccatca ccagacggac gtgtggcggg atcgcaatgg
    20101 caatctcccc ctgcaacgcg ccagggcgac gcagcgacca ctgggtaatg cccagttgct
    20161 gtaactgcca gtctcgtcgg gatgtcatag cgaatcgctc ctgttgtcag gggcgcaaat
    20221 atagcaaatt cgtcgatacc gcgccaacat atggctataa tcgccgccag tatcaattga
    20281 ggagcattcc atgtctgcat ttaccccggc aagtgaagtc ttgctgcgtc acagtgatga
    20341 tttcgaacaa agccgtattc tgtttgccgg agacttacag gatgacctgc ccgcgcgttt
    20401 agataccgcg gccagccgtg ctcataccca gcaattccac cactggcagg tgttaagccg
    20461 ccagatgggg gataacgccc gttttagcct ggtcgccacg gcagatgacg tcgcagattg
    20521 cgatacgctg atctactact ggccgaagaa caaaccggaa gcccagttcc agttgatgaa
    20581 tttactttct ctgcttccgg tgggcacgga tatttttgtc gttggcgaga accgcagcgg
    20641 cgtgcgcagc gccgagcaga tgctggccga ttatgcgccg ttgaataagg tagacagcgc
    20701 ccgtcgctgc ggcctctatt ttggtcgtct ggaaaagcag ccggtatttg atgccaataa
    20761 attctggggc gaatacagcg tcgatggctt gacggtcaaa acactgccgg gcgtgtttag
    20821 ccgcgacggt ctggatgtcg gcagtcagtt gctgctctcg acgctaactc cacacacgaa
    20881 aggtaaagtg ctggatgtcg gctgcggcgc gggagtgctt tccgttgctt ttgcgcgcca
    20941 ttcaccgaag atccgcctca ctctgtgtga tgtttctgca ccagcggtag aagccagccg
    21001 cgcaacactt gcgaccaacg gtgttgaagg tgaagtcttt gccagtaacg tcttttccga
    21061 ggtgaaaggt cgctttgata tgataatctc caacccgccg ttccacgatg ggatgcaaac
    21121 cagcctggat gccgcgcaaa cgctgattcg cggcgcggtg cgtcatctga acagcggcgg
    21181 cgagttgcga attgtagcga acgccttcct gccttacccg gacgtgctgg atgagacatt
    21241 tggtttccac gaagtgattg cgcaaaccgg gcgcttcaag gtgtatcgcg ccattatgac
    21301 ccgccaggcg aagaaaggtt gagtatctga tatatatcgc cgcataccgt gtcggatgcg
    21361 gcgtacacgc cttatctgac cgacaaacga attatccaca atccgctgat ggacattttt
    21421 ccagcaatta cactgctgtc gataattaac tattgacgaa aagctgaaaa ccactagaat
    21481 gcgcctccgt ggtagcaatt ctttttaaga attgatggta tgcgaaggtg gcggaattgg
    21541 tagacgcgct agcttcaggt gttagtgttc ttacggacgt gggggttcaa gtcccccccc
    21601 tcgcaccaaa cgaggcgata tcaaaaaaag taagatgact
//
LOCUS       DADSYM010000075        21533 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2415
            SAMN08039579-rid11723173.denovo.075, whole genome shotgun sequence.
ACCESSION   DADSYM010000075 DADSYM010000000
VERSION     DADSYM010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039579
            Sequence Read Archive: SRR6362533
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 21533)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21533)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980162.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 03:39:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,484
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 4,347
            CDSs (with protein)               :: 4,347
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 43 of 85
            Pseudo Genes (incomplete)         :: 37 of 85
            Pseudo Genes (internal stop)      :: 19 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21533
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039579-rid11723173.denovo.075"
                     /strain="LGE2415"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8302 N 95.9628 W"
                     /collection_date="2015-08-24"
     gene            341..793
                     /locus_tag="J1Y83_002475"
     CDS             341..793
                     /locus_tag="J1Y83_002475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF386 domain-containing protein"
                     /protein_id="HBA7630618.1"
                     /translation="MIVGNIHHLQSWLPEELREAIEYIKSHVSDETAKGKHAIDGDRL
                     FYLISEDTTEPGELRRAEYHARYLDIQIVLKGQEGMTFSTQPAGVPETDWLADKDIAF
                     IGQGIDEKTVILNEGDFVVFYPGEVHKPLCAVGAPAQVRKAVVKLLKS"
     gene            complement(838..1842)
                     /gene="argF"
                     /locus_tag="J1Y83_002476"
     CDS             complement(838..1842)
                     /gene="argF"
                     /locus_tag="J1Y83_002476"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HBA7630619.1"
                     /translation="MSGFYHKHFLKLLDFTPAELNSLLQLAAKLKADKKSGKEEARLT
                     GKNIALIFEKDSTRTRCSFEVAAYDQGARVTYLGPSGSQIGHKESIKDTARVLGRMYD
                     GIQYRGYGQEIVETLAEYAGVPVWNGLTNEFHPTQLLADLLTMQEHLPGKAFNEMTLV
                     YAGDARNNMGNSMLEAAALTGLDLRLVAPQACWPEAALVAECSALAQKHGGKITLTED
                     IASGVKGADFIYTDVWVSMGEPKEKWAERIALLRDYQVNSKMMALTGNSQVKFLHCLP
                     AFHDEQTTLGKKMAAEFGLYGGMEVTDEVFESPASIVFDQAENRMHTIKAVMVATLAK
                     "
     gene            2004..2420
                     /gene="rraB"
                     /locus_tag="J1Y83_002477"
     CDS             2004..2420
                     /gene="rraB"
                     /locus_tag="J1Y83_002477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E inhibitor RraB"
                     /protein_id="HBA7630620.1"
                     /translation="MANPEQLEEQREETRLIIDELLEDGSDPDALYTIEHHLSADDLE
                     TLEKAAVEAFKLGYEVTDPEELEVEDGDIVICCDILSECALNADLIDAQVEQLMTLAE
                     KFDVEYDGWGTYFEDPNGEDGDDEDFVDEDDDGIRH"
     gene            complement(2598..3101)
                     /locus_tag="J1Y83_002478"
     CDS             complement(2598..3101)
                     /locus_tag="J1Y83_002478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBA7630621.1"
                     /translation="MNVVASPALRLRKLTVADNPAIAHVIRQVSAEYGLTADKGYTVA
                     DPNLDELYQVYSQPGHAYWVVEYEGEVVGGGGIAPLTGSESDICELQKMYFLPAIRGK
                     GLAKKLALMAMEQAREMGFKRCYLETTAFLKEAIALYEHLGFEHIDYALGCTGHVDCE
                     VRMLRKL"
     gene            3294..4481
                     /locus_tag="J1Y83_002479"
     CDS             3294..4481
                     /locus_tag="J1Y83_002479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001622551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF898 family protein"
                     /protein_id="HBA7630622.1"
                     /translation="MNDVNIGKDNSRHSFVFTGKGGEYFLICLVNFLLTIITLGIYGP
                     WALIKCRRYIYQHVTLKGQPFSYKGTGGAIFVSMLLIVVVYLLSISCFAGQHFALGLF
                     LFALLICGIPCMAVKSLQYQANMTSLNDIRFGFNCSMMRAWWVMLGLPVLLALAFWLA
                     LYLIAQVTTSIGGLFFNLVALSLLSAIGLGVVHGITYSKWMPLLGNNATFGIHKFSIQ
                     VNVKECIKGCMLAILTMVPFIIVIGIMIAPVFQQLMMMTMLGRSDAGSEFVLQYYPQI
                     MASYFLYFVAILVFASYLYVTLRNLFLNNLTLANGTIRFHSSVTAIGMLLRMLAVLMG
                     SSITCGLAYPWLKMWMVSWIANNTHVQGDLDSLELTNDDKPQDSGSLMWISRGIMPYV
                     PFI"
     gene            complement(4528..7383)
                     /gene="valS"
                     /locus_tag="J1Y83_002480"
     CDS             complement(4528..7383)
                     /gene="valS"
                     /locus_tag="J1Y83_002480"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000416368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HBA7630623.1"
                     /translation="MEKTYNPQDIEQPLYEHWEKQGYFKPNGDESQESFCIMIPPPNV
                     TGSLHMGHAFQQTIMDTMIRYQRMQGKNTLWQVGTDHAGIATQMVVERKIAAEEGKTR
                     HDYGREAFIDKIWEWKAESGGTITRQMRRLGNSVDWERERFTMDEGLSNAVKEVFVRL
                     YKEDLIYRGKRLVNWDPKLRTAISDLEVENRESKGSMWHIRYPLADGAKTADGKDYLV
                     VATTRPETLLGDTGVAVNPEDPRYKDLIGKYVILPLVNRRIPIVGDEHADMEKGTGCV
                     KITPAHDFNDYEVGKRHALPMINILTFDGDIRESAQVFDTKGNESDVYSSEIPAEFQK
                     LERFTARKAVVAAVDALGLLEEIKPHDLTVPYGDRGGVVIEPMLTDQWYVRADVLAKP
                     AVEAVENGDIQFVPKQYENMYFSWMRDIQDWCISRQLWWGHRIPAWYDEAGNVYVGRN
                     EDEVRKENNLGADVVLRQDEDVLDTWFSSALWTFSTLGWPENTDALRQFHPTSVMVSG
                     FDIIFFWIARMIMMTMHFIKDENGKPQVPFHTVYMTGLIRDDEGQKMSKSKGNVIDPL
                     DMVDGISLPELLEKRTGNMMQPQLADKIRKRTEKQFPNGIEPHGTDALRFTLAALAST
                     GRDINWDMKRLEGYRNFCNKLWNASRFVLMNTEGQDCGFNGGEMTLSLADRWILAEFN
                     QTIKAYREALDSFRFDIAAGILYEFTWNQFCDWYLELTKPVMNGGTEAELRGTRHTLV
                     TVLEGLLRLAHPIIPFITETIWQRVKVLCGITADTIMLQPFPQYDASQVDEAALADTE
                     WLKQAIVAVRNIRAEMNIAPGKPLELLLRGCSADAERRVNENRGFLQTLARLESITVL
                     PADDKGPVSVTKIVDGAELLIPMAGLINKEDELARLAKEVAKIEGEISRIENKLANEG
                     FVARAPEAVIAKEREKLEGYAEAKAKLIEQQAVIAAL"
     gene            complement(7383..7826)
                     /gene="holC"
                     /locus_tag="J1Y83_002481"
     CDS             complement(7383..7826)
                     /gene="holC"
                     /locus_tag="J1Y83_002481"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004098129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit chi"
                     /protein_id="HBA7630624.1"
                     /translation="MKNATFYLLDNDTTVDGLSAVEQLVCEIAAERWRSGKRVLIACE
                     DEKQAYRLDEALWARPAESFVPHNLAGEGPRGGAPVEIAWPQKRSSSPRDILISLRTS
                     FADFATAFTEVVDFVPYEDSLKQLARERYKAYRVAGFNLNTATWK"
     gene            complement(7960..9471)
                     /gene="pepA"
                     /locus_tag="J1Y83_002482"
     CDS             complement(7960..9471)
                     /gene="pepA"
                     /locus_tag="J1Y83_002482"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HBA7630625.1"
                     /translation="MEFSVKSGSPEKQRSACIVVGVFEPRRLSPIAEQLDKISDGYIS
                     ALLRRGELEGKPGQTLLLHHVPNVLSERILLIGCGKERELDERQYKQVIQKTINTLND
                     TGSMEAVCFLTELHVKGRNNYWKVRQAVETAKETLYSFDQLKTNKSEPRRPLRKMVFN
                     VPTRRELTSGERAIQHGLAIAAGIKAAKDLGNMPPNICNAAYLASQARQLADSYSKNV
                     ITRVIGEQQMKELGMHSYLAVGQGSQNESLMSVIEYKGNASEDARPIVLVGKGLTFDS
                     GGISIKPSEGMDEMKYDMCGAAAVYGVMRMVAELQLPINVIGVLAGCENMPGGRAYRP
                     GDVLTTMSGQTVEVLNTDAEGRLVLCDVLTYVERFEPEAVIDVATLTGACVIALGHHI
                     TGLMANHNPLAHELIAASEQSGDRAWRLPLGDEYQEQLESNFADMANIGGRPGGAITA
                     GCFLSRFTRKYNWAHLDIAGTAWRSGKAKGATGRPVALLAQFLLNRAGFNGEE"
     gene            9738..10838
                     /gene="lptF"
                     /locus_tag="J1Y83_002483"
     CDS             9738..10838
                     /gene="lptF"
                     /locus_tag="J1Y83_002483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HBA7630626.1"
                     /translation="MIIIRYLVRETLKSQLAILFILLLIFFCQKLVRILGAAVDGDIP
                     ANLVLSLLGLGVPEMAQLILPLSLFLGLLMTLGKLYTESEITVMHACGLSKAVLVKAA
                     MILAVFTAIVAAVNVMWAGPWSSRHQDEVLAEAKANPGMAALAQGQFQQATNGSSVLF
                     IESVDGSDFKDVFLAQIRPKGNARPSVVVADSGHLTQLRDGSQVVTLNQGTRFEGTAL
                     LRDFRITDFQDYQAIIGHQAVALDPNDTDQMDMRTLWNTDTDRARAELNWRITLVFTV
                     FMMALMVVPLSVVNPRQGRVLSMLPAMLLYLLFFLIQTSLKSNGGKGKLDPTLWMWTV
                     NLIYLALAIVLNLWDTVPVRRLRASFSRKGAV"
     gene            10838..11920
                     /gene="lptG"
                     /locus_tag="J1Y83_002484"
     CDS             10838..11920
                     /gene="lptG"
                     /locus_tag="J1Y83_002484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313266.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HBA7630627.1"
                     /translation="MQPFGVLDRYIGKTIFTTIMMTLFMLVSLSGIIKFVDQLKKAGQ
                     GSYDALGAGMYTLLSVPKDVQIFFPMAALLGALLGLGMLAQRSELVVMQASGFTRMQV
                     ALSVMKTAIPLVLLTMAIGEWVAPQGEQMARNYRAQAMYGGSLLSTQQGLWAKDGNNF
                     VYIERVKGDEELGGISIYAFNENRRLQSVRYAATAKFDPEHKVWRLSQVDESDLTNPK
                     QITGSQTVSGTWKTNLTPDKLGVVALDPDALSISGLHNYVKYLKSSGQDAGRYQLNMW
                     SKIFQPLSVAVMMLMALSFIFGPLRSVPMGVRVVTGISFGFVFYVLDQIFGPLTLVYG
                     IPPIIGALLPSASFFLISLWLLMRKS"
     gene            complement(12081..13583)
                     /locus_tag="J1Y83_002485"
     CDS             complement(12081..13583)
                     /locus_tag="J1Y83_002485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF853 domain-containing protein"
                     /protein_id="HBA7630628.1"
                     /translation="MSEPLLIARTPDTELFLLPGMANRHGLITGATGTGKTVTLQKLA
                     ESLSEIGVPVFMADVKGDLTGIAQAGTTSEKLLARLKNIGVNDWQPHANPVVVWDIFG
                     EKGHPVRATVSDLGPLLLARLLNLNDVQSGVLNIIFRIADDQGLLLLDFKDLRAITQY
                     IGDNAKSFQNQYGNISSASVGAIQRGLLSLEQQGAAHFFGEPMLDIKDWMRTDANGKG
                     VINILSAEKLYQMPKLYAASLLWMLSELYEQLPEAGDLEKPKLVFFFDEAHLLFNDAP
                     QVLLDKIEQVIRLIRSKGVGVWFVSQNPSDIPDNVLGQLGNRVQHALRAFTPKDQKAV
                     KAVAQTMRANPTFDTEKAIQELGTGEALISFLDAKGSPSVVERAMVIAPCSRMGPVTE
                     DERNDLINHSPVYGKYEDDVDRESAYEMLQKGFQASIEQQNNPPAKGKEVAVDDGILG
                     GLKDILFGTTGPRGGKKDGVVQTMAKSAARQVTNQIVRGMLGSLLGGRRR"
     gene            complement(13661..14659)
                     /gene="idnR"
                     /locus_tag="J1Y83_002486"
                     /gene_synonym="gntH"
                     /gene_synonym="yjgS"
     CDS             complement(13661..14659)
                     /gene="idnR"
                     /locus_tag="J1Y83_002486"
                     /gene_synonym="gntH"
                     /gene_synonym="yjgS"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator IdnR"
                     /protein_id="HBA7630629.1"
                     /translation="MRNHRISLQDIATLAGVTKMTVSRYIRSPKKVAKETGERIAKIM
                     EEINYIPNRAPGMLLNAQSYTLGILIPSFQNQLFADILAGIESVTSVHNYQTLIANYN
                     YNRDSEEESVINLLSYNIDGIILSEKYHTIRTVKFLRSATIPVVELMDVQGERLDMEV
                     GFDNRQAAFDMVCTMLDKRVRRKILYLGSKDDTRDEQRYQGYCDAMMLHNLSPLRMNP
                     RAISSIHLGMQLMRDALSANPDLDGVFCTNDDIAMGALLLCRERNLAVPEQISIAGFH
                     GLEIGRQMIPSLASVITPRFDIGRMAAQMLLSKIKNNDHNHNTVDLGYQIYHGNTL"
     gene            complement(14726..16045)
                     /gene="idnT"
                     /locus_tag="J1Y83_002487"
     CDS             complement(14726..16045)
                     /gene="idnT"
                     /locus_tag="J1Y83_002487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gnt-II system L-idonate transporter"
                     /protein_id="HBA7630630.1"
                     /translation="MPLIIIAAGVALLLILMIGFKVNGFIALVLVAAVVGFAEGMDAQ
                     AVLHSIQNGIGSTLGGLAMILGFGAMLGKLISDTGAAQRIATTLIATFGKKRVQWALV
                     ITGLVVGLAMFFEVGFVLLLPLVFTIVASSGLPLLYVGVPMVAALSVTHCFLPPHPGP
                     TAIATIFEANLGTTLLYGFIITIPTVIVAGPLFSKLLTRFEKAPPEGLFNPHLFSEEE
                     MPSFWNSIFAAVIPVILMAIAAVCEITLPKTNTVRLFFEFVGNPAVALFIAIVIAIFT
                     LGRRNGRTIEQIMDIIGDSIGAIAMIVFIIAGGGAFKQVLVDSGVGQYISHLMTGTTL
                     SPLLMCWTVAALLRIALGSATVAAITTAGVVLPIINVTHADPALMVLATGAGSVIASH
                     VNDPGFWLFKGYFNLTVGETLRTWTVMETLISIMGLLGVLAINAVLH"
     gene            complement(16110..16874)
                     /gene="idnO"
                     /locus_tag="J1Y83_002488"
     CDS             complement(16110..16874)
                     /gene="idnO"
                     /locus_tag="J1Y83_002488"
                     /EC_number="1.1.1.69"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 5-dehydrogenase"
                     /protein_id="HBA7630631.1"
                     /translation="MNDLFSLAGKNILITGSAQGIGYLLATGLGKYGAQIIINDITAE
                     RAELAVEKLHQEGIQAVAAPFNVTHKHEIDAAVEHIEKDIGPIDVLVNNAGIQRRHPF
                     TEFPEQEWNDVIAVNQTAVFLVSQAVTRHMVERKAGKVINICSMQSELGRDTITPYAA
                     SKGAVKMLTRGMCVELARHNIQVNGIAPGYFKTEMTKALVEDEAFTAWLCKRTPAARW
                     GDPQELIGAAVFLSSKASDFVNGHLLFVDGGMLVAV"
     gene            complement(16898..17929)
                     /locus_tag="J1Y83_002489"
     CDS             complement(16898..17929)
                     /locus_tag="J1Y83_002489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-idonate 5-dehydrogenase"
                     /protein_id="HBA7630632.1"
                     /translation="MQVKTQSCVVAGKKTVAVTEQTIDWNNNGTLVQITRGGICGSDL
                     HYYQEGKVGNFMIKAPMVLGHEVIGKVIHSDSSELHEGQTVAINPSKPCGHCKYCIEH
                     NENQCTDMRFFGSAMYFPHVDGGFTRYKMVETSQCVPYPAKADEKVMAFAEPLAVAIH
                     AAHQAGELQGKRVFVSGVGPIGCLIVSAVKTLGAAEIVCADVSPRSLSLAKEMGADVL
                     VNPQNDDMDHWKAEKGHFDVSFEVSGHPSSVNTCLEVTRARGVMVQVGMGGAMAEFPM
                     MTLIGKEISLKGSFRFTSEFNTAVSWLANGVINPLPLLSAEYLFTDLEEALRFAGDKT
                     QAAKVQLVF"
     gene            18146..18709
                     /gene="idnK"
                     /locus_tag="J1Y83_002490"
     CDS             18146..18709
                     /gene="idnK"
                     /locus_tag="J1Y83_002490"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HBA7630633.1"
                     /translation="MAGESFILMGVSGSGKTLIGSKVAALLSAKFIDGDDLHPAKNID
                     KMSQGIPLSDEDRLPWLERLNDASYSLYKKNETGFIVCSSLKKQYRDILRKGSPHVHF
                     LWLDGDYETILARMQRRAGHFMPVALLKSQFEALERPQEDEQDIVRIDINHDIANVTE
                     QCRQAVLAIRQNRICAKEGSASDQRCE"
     gene            complement(18713..19732)
                     /gene="ahr"
                     /locus_tag="J1Y83_002491"
     CDS             complement(18713..19732)
                     /gene="ahr"
                     /locus_tag="J1Y83_002491"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent aldehyde reductase Ahr"
                     /protein_id="HBA7630634.1"
                     /translation="MSMIKSYAAKEAGGELEVYEYDPGELKPQDVEVQVDNCGICHSD
                     LSMIDNEWGFSQYPLVAGHEVIGRVVALGSAAQDKGLQVGQHVGIGWTARSCGHCDAC
                     ISGNQINCEQGAVPTIMNRGGFAEKLRADWQWVIPLPENIDIESAGPLLCGGITVFKP
                     LLMHHITATSRVGVIGIGGLGHIAIKLLHAMGCEVTAFSSNPAKEQEVLAMGADKVVN
                     SRDPQALKALAGQFDLIINTVNVSLDWQPYFEALTYGGNFHTVGAVLTPLSVPAFTLI
                     AGDRSVSGSATGTPYELRKLMRFAARSKVAPTTELFPMSKINDAIQHVRDGKARYRVV
                     LKADF"
     gene            19928..20012
                     /locus_tag="J1Y83_002492"
     tRNA            19928..20012
                     /locus_tag="J1Y83_002492"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:19962..19964,aa:Leu,seq:caa)
     gene            20213..21472
                     /locus_tag="J1Y83_002493"
     CDS             20213..21472
                     /locus_tag="J1Y83_002493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008815333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase arm-type DNA-binding domain-containing
                     protein"
                     /protein_id="HBA7630635.1"
                     /translation="MALTDTKVRSAKPEEKEYSLVDGDGMSLLVKPGGSKYWRFRFRF
                     GGKQHLMAFGVYPDVSLADARKKREEARKLVAAGIDPREHKRAVKEEQAKEIITFEKV
                     AREWLVTNQKWSEDHANRVKKSLEDNIFPAIGSRNIAELGTRDLLIPIKAVEKSGCLE
                     VASRLQQRTTAIMRYAVQSGLIDYNPAQEMAGAVASSNRQHRPALELKRIPELLQKID
                     EYTGRPLTRWATELTLLIFIRSSELRFARWSEIDFETSMWTIPPEREPIPGVKHSQRG
                     SKMRTPHLVPLSKQALAILKQIKQFCGEHELIFIGDHDPRKPMSENTVNSALRVMGYD
                     TKVEVCGHGFRTMACSSLIESGLWSKDAVERQMSHMERNSVRAAYIHKAEHLDERKLM
                     LQWWADFLDANREKGISPFDFAKQAAR"
BASE COUNT         5257 a         5434 c         5366 g         5476 t
ORIGIN      
        1 tatctatgcg aaaggataag gtgattattt ttcacagcac tattttcacg ggataactat
       61 gacagcacgg gcattaaaag ctaagaaaat gaactgtgat ctgactgaga gttttctttt
      121 aacatatgca ataacaagat attaggacca aaatacatag ctaagttata agatttatgc
      181 agcaaaactg catacaaaat tactcctgct taacatcagt acaacatctc cagcaataaa
      241 catgcgcaag ggcaaaacct gatgattttt ctatggtgag aggtggcttt ttttcgtata
      301 aggaaagaag tgaaacattg ttttaataaa ggaagatatt atgattgttg gcaacatcca
      361 tcacttacaa tcctggctgc cggaagaact tcgcgaagca attgagtaca tcaaatcaca
      421 tgtcagcgat gagacagcaa aaggtaagca cgctattgac ggcgaccgtc tgttttatct
      481 gatttctgag gatacgactg aaccaggtga actgcgtcgt gctgagtatc atgctcgtta
      541 tctcgatatc cagattgtat taaaagggca ggaagggatg acatttagca cgcaaccagc
      601 aggcgtacca gagactgact ggctggcaga taaagatatt gcttttatcg ggcaggggat
      661 cgatgaaaaa acagtgattc ttaatgaagg ggattttgtc gttttctatc ctggcgaagt
      721 tcacaaaccg ctttgtgcgg tgggagcccc cgctcaggta cggaaagcag tagttaagct
      781 attaaaatcg taagtgatca ctgccggggg cgacctgtgc cgccgcccgg caaaacgtta
      841 ttttgctaat gtagcgacca taacagcttt tatggtgtgc atccggtttt ctgcctggtc
      901 aaagacaata cttgccggag attcaaagac ttcatcggtg acttccattc caccatacag
      961 accgaattct gcggccattt ttttacccag cgtggtttgc tcatcatgaa atgcaggcag
     1021 acagtggagg aacttaacct gcgaattacc ggtcagggcc atcattttgc tattcacctg
     1081 atagtcacgc agcagggcaa tacgctccgc ccatttctct ttgggctcac ccatcgatac
     1141 ccagacatcg gtatagataa agtctgcgcc tttaacccca gaggcgatat cttccgtcaa
     1201 ggtgatttta cccccgtgtt tttgtgccag agcactgcat tcggcgacca gcgcagcttc
     1261 cggccagcat gcctgtggcg cgaccagacg caaatccaga ccggtaagcg ccgcagcttc
     1321 gagcatcgaa ttgcccatgt tgttacgcgc gtcacctgca tagaccagcg tcatttcgtt
     1381 gaatgctttg ccgggcaaat gctcctgcat ggtgaggaga tccgccagca gctgcgtggg
     1441 gtggaactca ttggtcaggc cgttccatac tggcacgcca gcgtattccg ccagtgtttc
     1501 gacaatctcc tgaccatagc cgcgatactg aataccgtca tacatgcgac caagcacgcg
     1561 ggcagtgtct ttaatcgact ctttgtgacc aatctggctg ccgcttgggc cgagataagt
     1621 aacgcgagca ccctggtcat atgcggcaac ttcgaaagag catcgggtac gggtcgagtc
     1681 tttttcgaag atgagcgcga tgtttttgcc agtgagtctg gcttcttctt taccgctttt
     1741 cttatcggct ttcagcttcg cggctaactg caacaagctg ttgagttcag ctggcgtgaa
     1801 atcgagtaat ttcaggaaat gcttatgata aaacccggac atagatccct cctgtggcta
     1861 acgccttaat gaattaaaat tcaatttata tgtatgatta ttcatttgca agtctaaagc
     1921 ataaatcttt gtcacaaagg tggaggcaat gtcagtggtg tgtgacaata agagtatcgg
     1981 caggacatta agaggaatga gccatggcaa acccggaaca actggaagaa cagcgtgaag
     2041 aaacacgttt gattattgac gaattactgg aagatggcag cgatccggac gcgctgtaca
     2101 ccatcgaaca ccatctttcc gcagacgatc tggaaaccct ggaaaaagca gcagttgaag
     2161 cgtttaaact cggttacgaa gtgaccgatc cagaagagct ggaagttgaa gatggtgata
     2221 tcgtgatttg ctgcgacatc ctcagcgagt gtgcgttgaa cgccgacctg atcgatgccc
     2281 aggttgaaca actgatgacg ctggcggaga aatttgacgt tgagtacgat ggttggggca
     2341 cttactttga agatcctaac ggcgaagacg gcgacgatga agattttgtc gatgaagacg
     2401 atgacggtat tcgtcactaa ttaagcgttg acgacaaagg ctgcggtacc tgatgcgcta
     2461 cgcatatcag gcccaagtag ttcctgcaac ctggtgaatt tgtagcccgg ataaggcatt
     2521 tacgtcacat ccagcatact caaaaagcac gtggttaatg ttcgaatgcc cgcgagtcta
     2581 agctcgcggg cattttttca aagtttgcgt agcatccgta cttcacaatc gacatgaccc
     2641 gtgcagccaa gcgcatagtc gatatgctca aagcccaaat gctcataaag cgcaatggct
     2701 tcctttaaaa aagcggtcgt ttccagatag cagcgtttga aacccatttc tcgcgcctgc
     2761 tccattgcca ttaaggccag tttttttgcc agccctttgc cgcggatagc cggaagaaaa
     2821 tacatctttt gcagttcgca aatgtctgac tcactcccgg ttaatggcgc aatcccaccg
     2881 ccgccgacca cttcaccctc gtactcaacc acccaatatg cgtggccagg ctgactatac
     2941 acttgataca gctcgtcaag gttcggatcg gcgacggtgt agcctttatc agcggtaaga
     3001 ccgtattcgg cggatacctg acgaatgacg tgggcgatag caggattatc ggcgacggta
     3061 agtttgcgca aacggagtgc gggagaagcg acaacattca tagcatagct caatttcaaa
     3121 tgtatgattt atagctcact ttaataccac cctcgcttag gtggtgcaag aagaagataa
     3181 atcagaaaat attacgcata tctctctgag ataaagcgta ttctaggcga accagtatca
     3241 attgactgga attattatcg gtatgaatgg aatttatttt agggatatag ttcatgaatg
     3301 atgtaaacat cgggaaagat aattctcggc actcgtttgt tttcacaggc aaaggtggtg
     3361 agtattttct tatttgtctt gtgaattttt tactgacaat tattactctg ggaatatatg
     3421 gaccgtgggc attgataaaa tgccgtcgtt atatttatca acatgtgaca ctcaaggggc
     3481 aaccatttag ttataaggga acaggtggtg ccatttttgt tagcatgtta cttattgtgg
     3541 tggtttatct tctcagtatt tcttgttttg caggtcaaca ctttgcgctc ggtttattcc
     3601 tgtttgctct cttgatctgc ggtattcctt gtatggcagt gaagagtctg cagtatcagg
     3661 cgaacatgac ctcactgaat gacattcgct ttggctttaa ctgctcaatg atgcgtgcat
     3721 ggtgggttat gttaggtctg ccagttttgt tggcattagc tttttggctc gcactgtacc
     3781 ttattgcgca agtgacgaca tcgataggag gcttattctt taacttagtc gcattaagtt
     3841 tgctttctgc catcgggcta ggtgttgtcc atggtataac ctatagtaaa tggatgccat
     3901 tgctgggtaa taatgcaact tttggcattc ataagttttc tattcaggta aacgtaaaag
     3961 aatgcattaa aggttgcatg ctcgcaatat taaccatggt tcctttcatc attgtgattg
     4021 gtattatgat cgcaccagta ttccagcagt taatgatgat gaccatgttg ggtaggtctg
     4081 atgctggcag tgagtttgta ttacaatatt acccgcagat tatggcttct tatttcctgt
     4141 attttgtcgc gatccttgtt tttgccagtt atctctatgt cacgttacgc aatctgtttt
     4201 tgaataatct gacgctggca aatgggacaa ttcgttttca ttcatctgtt accgcgatcg
     4261 gtatgcttct gcgcatgttg gcggtattaa tggggtcctc aattacctgt ggtctggctt
     4321 atccctggct gaaaatgtgg atggttagct ggattgctaa caatacccat gtccagggcg
     4381 accttgattc gctggagtta accaatgatg acaaaccgca ggatagcggt tcgttaatgt
     4441 ggatatctcg cggtattatg ccgtatgttc cgtttatcta aataaaaagg ccggagcatg
     4501 ctccggcctt cgttttcact atgagtatta cagcgcggcg ataacagcct gctgttcaat
     4561 cagcttcgct ttcgcttctg cgtagccttc cagcttctca cgctctttcg cgatgaccgc
     4621 ttccggtgcg cgggcgacaa agccttcgtt cgccagcttg ttctcaatac ggctgatttc
     4681 gccttcgatc ttcgctactt ctttcgccag acgtgccagc tcatcttctt tgttgatgag
     4741 gcctgccatc gggatcagca gttctgcgcc gtcgacgatc tttgtaacgg aaaccggacc
     4801 tttatcatca gcaggcagca cggtgatgct ttccagacgc gccagcgttt gcaggaagcc
     4861 gcggttttca tttacgcgac gttctgcatc cgcgctgcaa ccacgcagca gcagctccag
     4921 cggtttaccc ggcgcgatgt tcatttctgc acggatgtta cgtacagcaa cgatcgcctg
     4981 tttcagccac tcggtgtcgg ccagtgccgc ttcatcaacc tgagacgcat cgtactgcgg
     5041 gaacggctgc agcatgatgg tgtcggcagt gataccgcaa agtactttca cacgctgcca
     5101 gatggtttcg gtgatgaacg gaatgatcgg atgcgcgagg cgcagcagac cttccagtac
     5161 agtcaccagc gtatggcgag taccgcgcag ttcggcttct gttccaccgt tcattaccgg
     5221 cttggtcagc tccagatacc agtcacagaa ctggttccag gtgaattcat acagaatgcc
     5281 tgcggcgata tcgaagcgga agctgtccag cgcttcgcgg tacgctttga tggtctggtt
     5341 gaactccgcc agaatccagc gatccgccag cgacagcgtc atttcaccgc cgttgaagcc
     5401 gcaatcctga ccttccgtgt tcatcagcac aaaacggctg gcgttccaca gcttgttaca
     5461 gaagttacgg tagccttcca gacgcttcat atcccagttg atgtcacgac cggtagaagc
     5521 cagcgccgcc agggtgaagc gcagcgcgtc agtaccgtgc ggctcaatgc cgttcgggaa
     5581 ctgcttctcg gtgcgcttac ggattttgtc cgccagctgc ggctgcatca tattgccggt
     5641 acgtttttcc agcagttctg gcagcgaaat accgtcaacc atatccagcg ggtcgataac
     5701 gttacccttg gatttggaca tcttctggcc ttcgtcgtca cgaatcagac cggtcatgta
     5761 aacagtgtgg aacggcacct gcggtttgcc attttcatct ttgatgaagt gcatggtcat
     5821 catgatcatg cgggcaatcc agaagaagat gatgtcgaaa ccagatacca tcacgctggt
     5881 tgggtggaac tgacgcaggg cgtcggtatt ttccggccag ccaagggtag agaaggtcca
     5941 cagcgcagaa gagaaccagg tgtcgagaac gtcttcgtcc tgacgcagga caacatcagc
     6001 accgaggtta ttttctttac gcacttcgtc ttcgttgcgg ccaacataaa cgttacccgc
     6061 ttcgtcgtac catgccggga tacggtgacc ccaccacaac tgacgagaga tacaccagtc
     6121 ctgaatatcg cgcatccagg agaagtacat gttttcgtac tgcttcggta cgaactgaat
     6181 gtcgccgttc tcaaccgctt caactgccgg tttcgccagt acatcggcac gcacgtacca
     6241 ctggtcggtc agcatcggtt cgataaccac gccgccacgg tcgccgtaag gaacggtcag
     6301 gtcgtgcggt ttaatttctt ccagcaggcc gagtgcatca accgcagcaa cgacggcttt
     6361 acgtgcagta aaacgctcga gtttctggaa ctctgccggg atttcgctgg aataaacgtc
     6421 agattcgtta cctttggtat cgaacacctg ggcgctttca cggatatcac cgtcaaaggt
     6481 caggatgttg atcatcggca gcgcgtgacg tttaccgact tcatagtcgt taaagtcgtg
     6541 cgccggagtg attttcacgc agccggtgcc tttttccatg tcggcgtgtt cgtcgccaac
     6601 gatcggaata cgacggttaa ccagcggcag aatgacatat ttgccaatca gatctttgta
     6661 acgcggatct tccgggttaa cggctacgcc ggtatcgccc agtagggttt ccggacgggt
     6721 agtcgcaacc accagataat ctttaccatc tgcggttttc gcgccgtcag ccagcggata
     6781 gcggatgtgc cacatcgaac ctttcgattc gcggttttcc acttccaggt cagagatagc
     6841 ggtgcgcagt ttcgggtccc agtttaccag gcgcttgcca cggtaaatca ggtcttcttt
     6901 atacagacga acgaaaactt ctttcaccgc attggacaga ccttcgtcca tggtgaagcg
     6961 ttcacgctcc cagtccacgg agttgccgag acggcgcatc tgacgggtaa tggtgccgcc
     7021 ggattccgct ttccattccc agattttgtc gatgaaagct tcgcggccgt agtcgtgacg
     7081 ggttttacct tcttctgcgg caatcttgcg ctcaacgacc atctgggtag cgatcccggc
     7141 gtggtcagta ccgacctgcc acagggtgtt tttgccctgc atgcgctgat agcggatcat
     7201 ggtatccatg atggtttgct ggaaggcgtg acccatatgc aaactgccgg tgacgttcgg
     7261 cggcgggatc atgatgcaga aactttcctg gctttcatcg ccattaggct taaagtagcc
     7321 ctgcttttcc cagtgctcgt aaagcggctg ttcgatatct tgtgggttat atgtcttttc
     7381 cattatttcc aggttgccgt attcaggttg aaaccagcca cgcggtaggc tttatagcgt
     7441 tcgcgcgcca gttgtttcag agaatcttca taaggaacga agtctaccac ttctgtgaaa
     7501 gcggtggcaa aatctgcaaa gcttgttcgc agactaatca atatatcgcg cgggctgctg
     7561 ctgcgttttt gcggccaggc gatctccacc ggcgcaccgc cgcgcggtcc ttctcccgct
     7621 aaattatgcg ggacaaagct ttctgccgga cgcgcccaca gggcttcatc cagccggtaa
     7681 gcctgctttt catcttcaca ggcgatgagc acgcgcttac cgctgcgcca acgttctgcg
     7741 gcaatttcac acaccagttg ctcaacggcg cttaagccat cgacggtggt gtcattgtcc
     7801 agaaggtaga acgttgcgtt tttcatatat ggggcttctt gtggtggatt taaatattgc
     7861 tgcattcctc accctgaaag atgagcaatg aatttctatt gcgctcgatc ggttccctct
     7921 cccttaggga gagggttagg gtgagggtaa aacgcaaact tactcttcgc cgttaaaccc
     7981 agcgcggttt aacaggaact gtgccagcaa cgctaccgga cgaccggttg cgccttttgc
     8041 tttaccagaa cgccaggcgg ttcctgcaat atccaggtgc gcccagttgt acttacgggt
     8101 aaagcgtgac aggaagcaac ctgcggtaat cgctccacca ggacgaccgc caatgttcgc
     8161 catatcggca aaattggact ccagttgttc ctgatactcg tcacccagcg gtaagcgcca
     8221 tgcgcggtca ccggattgtt cagacgcggc aatcagttca tgggccagcg gattatggtt
     8281 cgccatcaga ccagtaatat gatgacccag cgcgatcacg caggcaccgg tcagcgtcgc
     8341 cacatcaatc accgcttccg gctcaaaacg ttcaacgtaa gttaacacgt cgcacagtac
     8401 caggcggcct tcggcatcgg tgttcagcac ttcaacggtt tgaccggaca tggtggttaa
     8461 cacatcgccc ggacgatagg cacgcccgcc aggcatgttt tcgcagcctg ccagcacgcc
     8521 aataacgtta atcggcagtt gcagctccgc gaccatccgc atcacaccgt aaaccgctgc
     8581 cgcaccgcac atatcgtact tcatctcatc catgccttct gaaggcttga tggagatacc
     8641 gccggagtcg aaggttaaac ctttacccac cagcacgatt gggcgtgcat cttccgacgc
     8701 gttgcctttg tactcaatca ctgacatcag cgattcgttt tgcgaaccct gaccgacagc
     8761 cagataggaa tgcatcccca gctctttcat ctgttgttcg ccgataacgc gggtgatgac
     8821 attcttgctg tagctgtcag ccaactggcg cgcctgagag gcgaggtaag cagcgttaca
     8881 gatattcggt ggcatattgc cgagatcttt cgcggcttta atccctgcgg caatcgccag
     8941 accatgctgg atcgcgcgtt caccgctggt cagctcacgg cgggtcggca cgttgaacac
     9001 cattttacgc agcggacgac gcggctcgct cttgttcgtt ttcaactgat cgaaactgta
     9061 gagcgtctct tttgccgtct cgacagcctg acgcactttc cagtagttgt tacggccttt
     9121 aacgtgtagt tcagtcagaa agcagaccgc ttccattgag ccagtatcat tcagcgtatt
     9181 aatggttttc tgaataacct gcttgtactg acgctcatcc agctcacgtt ctttgccgca
     9241 accaataagg aggatgcgtt cagaaagcac gtttggcaca tggtgcagca acaatgtctg
     9301 ccccggtttt ccttccagtt cgccccgacg tagcagggcg ctgatgtacc catcgctgat
     9361 tttatcgagc tgttctgcaa tcggagaaag gcgacgtggt tcgaagacgc ccacgacgat
     9421 gcaggcactc cgctgtttct ccgggctacc gctttttaca ctaaactcca tgcactacgc
     9481 tcctgaatct taaagacaac ggcggtggct acagatagaa ttgcaagctt tcgtaactca
     9541 tgtccgctgt tgcgatgact tcgtgttaat cttaacgtta ttacggcatt ggcacgtcag
     9601 aacaaaatct gagaggtgaa tccgttgagt ataattatct tagcgacgat ttcgacgact
     9661 caagagaata aatgacgttt aagccatgaa acaagctaaa atcctgcaaa agacgagttt
     9721 ttacgggcgt atttaaagtg ataatcataa gatatctggt gcgggagacg ctcaaaagcc
     9781 agctggcgat actcttcatc ttgcttttga tcttcttctg tcaaaagtta gtgaggatcc
     9841 tcggcgcagc ggttgacggc gatattccgg cgaacctggt gctctccctt ctcgggttgg
     9901 gcgtgccgga aatggcgcag cttatcctgc cattaagcct gttcctcggg ctgctgatga
     9961 cgctgggcaa actgtatacc gaaagtgaaa ttacggtaat gcatgcctgc ggtctgagca
    10021 aagcggtgct ggtgaaagcg gcaatgatcc ttgcggtgtt cacggcaatc gtcgcggcgg
    10081 ttaacgtgat gtgggcggga ccatggtcat cgcgtcatca ggatgaagtg ttagcagaag
    10141 cgaaagcgaa ccctggcatg gcggcgctgg cacaagggca attccagcaa gcgactaatg
    10201 gcagctcggt gctgttcatc gaaagcgttg acggcagcga tttcaaagat gtgttcctcg
    10261 cgcaaattcg accaaaaggt aatgcacgac cttctgtggt ggtggccgat tccggacatt
    10321 taacccagct gcgcgacggc tcccaggtcg tcactctcaa ccagggaacg cgcttcgaag
    10381 gcactgcact gttacgtgat ttccgcatta cggatttcca ggattatcag gcgatcattg
    10441 gtcaccaggc ggtggcgctc gacccgaacg ataccgacca gatggacatg cgcacattgt
    10501 ggaacactga caccgatcgt gctcgcgcag aactgaactg gcgtatcacg ttggtgttca
    10561 ccgtgtttat gatggcactt atggtcgtac cgctgagcgt ggttaacccg cgtcaggggc
    10621 gcgtactatc gatgctgcca gccatgctgc tgtatctgtt gttcttcctg atccagacct
    10681 ccctgaaatc gaacggcggt aaaggtaagc tggacccgac gctgtggatg tggaccgtta
    10741 acctgattta tctggctttg gcgattgttc tcaacctttg ggacaccgtg ccggtccgcc
    10801 gcctgcgcgc cagtttttcg cgtaaaggag cggtgtgatg caaccttttg gcgtacttga
    10861 ccgctatatc ggtaaaacta ttttcaccac catcatgatg acgctgttca tgctggtgtc
    10921 gctgtccggc attatcaagt ttgtcgatca gctgaaaaaa gccgggcagg ggagttacga
    10981 cgcgttaggc gcaggaatgt ataccttgct gagcgtgccg aaagatgtgc aaatcttctt
    11041 cccgatggcg gctctgcttg gggcgttgct tggtcttggg atgctggcgc agcgcagcga
    11101 actggtggtg atgcaggctt ctggttttac ccgtatgcag gtggcgctgt cggtgatgaa
    11161 aaccgccatt ccgctggtct tgctgacgat ggcgattggt gaatgggtcg cgccgcaggg
    11221 cgagcagatg gcgcgtaact accgtgcgca ggcgatgtac ggcggctcgt tgctctctac
    11281 ccagcaaggt ttatgggcga aagatggcaa caacttcgtc tacattgagc gggttaaagg
    11341 tgacgaagag ttaggtggca tcagtattta tgcctttaac gagaatcgtc gtctgcaatc
    11401 cgtacgctat gccgctaccg cgaagtttga cccagaacat aaagtctggc gtctgtcgca
    11461 ggttgatgaa tctgatttga ccaatccgaa acagatcacc ggttcgcaga cggtgagcgg
    11521 cacctggaaa accaacctca cgccagacaa actgggcgtg gtggcgctgg acccggatgc
    11581 actctctatc agcggtttgc acaactatgt gaagtatctg aagtcgagcg gtcaggatgc
    11641 cggacgttat cagctcaaca tgtggagcaa aatcttccag ccgctatccg tggcggtgat
    11701 gatgctgatg gcgctgtcgt tcatctttgg cccactgcgt agcgtaccga tgggcgtgcg
    11761 tgtggtcact ggtattagct tcggttttgt cttctacgta ctggaccaga tcttcggccc
    11821 gctgacgttg gtttacggca tcccaccgat catcggcgca ctgttgccaa gtgccagctt
    11881 cttcttaatc agcctgtggc tgttaatgag aaaatcgtaa ccgaatgaac gaataaaggg
    11941 agcgaaagct ccctttatta ttgttagcaa agtgtgtttc gttcatgcct gaaaaatcat
    12001 gaaaattcaa tatattgcag tagtgtaggc ctgatgcggc gcgccagcgt tgcatcaggc
    12061 aaagacaaat cgcacctggc ttaccttctt ctccccccca gcaaactccc caacatcccg
    12121 cgcacaatct gattcgtcac ctggcgggcg gcgctttttg ccattgtttg caccacacca
    12181 tctttcttcc cgccgcgtgg tccggtagtg ccgaacaaaa tatctttcaa tccaccgaga
    12241 ataccgtcat ccaccgccac ctctttccct ttcgcgggag gattattttg ctgctcgata
    12301 ctggcctgaa agcctttttg caacatctca taggcggatt cccggtccac gtcatcctcg
    12361 tacttgccat acaccgggga gtggttaatc aagtcattgc gctcatcttc cgtcaccggc
    12421 cccattcgtg aacagggggc aatcaccatc gcccgttcca ccactgaagg gcttcctttc
    12481 gcatcgagaa acgagattaa cgcctcgccg gtccccagtt cctggattgc cttttcggta
    12541 tcaaatgttg gattggcccg catggtttgc gctactgcct tcactgcttt ctgatcttta
    12601 ggcgtaaaag cacgcaaagc gtgttgaacg cgattaccga gctgccctag cacgttatcc
    12661 gggatatctg acgggttttg cgaaacgaac cagacgccca cgccttttga gcgaataagc
    12721 cttatcacct gctcaatctt atccagcagt acctgcggtg catcgttaaa cagcagatgt
    12781 gcttcatcga agaaaaacac cagttttggt ttctccagat cgcccgcttc cggcaattgt
    12841 tcatacaact ctgaaagcat ccacagcagg ctggcggcgt acagtttcgg catctgataa
    12901 agcttctcgg cgctgaggat attgataacg cctttaccgt tggcatcggt gcgcatccag
    12961 tctttgatat ccagcatcgg ctcaccaaag aagtgtgctg cgccttgctg ttccagcgac
    13021 aacagcccgc gctggatggc accaaccgat gcactactga tattaccgta ctgattctgg
    13081 aaggatttgg cgttatcgcc gatgtactgg gtaattgccc gcagatcttt aaagtcgagc
    13141 agcaacaatc cttgatcgtc agcaatgcgg aaaatgatat tcagcacgcc agattgcaca
    13201 tcgttgagat tcaacagccg cgccagcaac agcggcccca gatccgaaac cgtcgcccgc
    13261 accgggtggc ctttctcgcc aaagatatcc cacaccacca ccggattggc atgcggttgc
    13321 cagtcattga caccgatatt tttaagcctt gcgagcagtt tttccgaggt cgttcctgcc
    13381 tgcgcgatgc cggtcagatc gcctttcaca tcggccataa acaccggaac accgatttcc
    13441 gacaatgact ctgccagttt ttgcagcgtg acagttttac ccgtccccgt tgcgccagta
    13501 atcagcccgt gacggttagc cattcccggc agtaaaaaca gttctgtgtc cggcgtgcgg
    13561 gcaattaaca ggggttcgct catgagattt cctccagttt cctgcctgga gtataggcaa
    13621 cgtccggcaa agagaatatg tgtaatgcag agaaaataac ctaaagcgtg ttaccgtgat
    13681 aaatctgata cccgaggtca acagtgttgt ggttgtgatc gttattctta attttgctca
    13741 gcaacatttg ggcggccatc cgaccaatat caaaacgggg agtaattacg ctggcgaggc
    13801 ttgggatcat ttgcctgcca atctccagcc catgaaaacc cgcaatggaa atttgttctg
    13861 gcacggcgag gtttcgctcg cggcacagca gaagcgcacc catcgcaata tcgtcattgg
    13921 tgcaaaatac accgtctaaa tccgggtttg cgctaagggc atcacgcatt aactgcattc
    13981 ctaaatgaat ggatgagatg gcgcgtggat tcatgcgtaa tggggaaaga ttatgcagca
    14041 tcatcgcatc gcaatacccc tgataacgct gttcatcgcg ggtgtcatct ttggaaccga
    14101 gatacagaat tttgcgcctg actcgcttat ccagcatggt acacaccatg tcaaaagcag
    14161 cctgccgatt atcaaaaccg acctccatat ccagccgttc tccctgtaca tccatcaatt
    14221 ccacgaccgg aatggtggct gaacgcagaa atttcaccgt cctgatggtg tgatattttt
    14281 ccgaaagaat aatcccgtca atgttataag agagcaggtt aatcaccgac tcttcttcgg
    14341 aatcgcgatt gtaattgtaa ttagcaataa gggtctgata attatgcaca gaggtgacgg
    14401 attctattcc cgcgagaatg tcagcgaaga gttgattttg aaatgacgga atcaagatac
    14461 cgagggtata actttgtgca ttcaacaaca tgccgggagc gcgattaggg atataattga
    14521 tctcttccat aattttggcg atgcgctcgc ctgtttcctt tgccaccttt ttcggcgagc
    14581 ggatataacg actcacggtc atttttgtta cgccagccag cgtagcgata tcctgtaaag
    14641 aaattctgtg attgcgcata acttctccca acgtcggggg aatcttcaat cttcccccga
    14701 aactacatca caatatttta ttctttcagt gcaatacggc gttaatggct aacacgccca
    14761 gcaaacccat aatagaaata agggtttcca tcaccgtcca ggtacgcaac gtttcaccaa
    14821 ccgtcagatt aaaataccct ttaaatagcc agaagccagg gtcgtttacg tgtgacgcga
    14881 tcacgctgcc cgcaccagtt gccagtacca ttaaagcggg atcggcatgg gtaacgttga
    14941 taatcggcaa caccacaccc gcggtggtaa tggccgcgac ggtggcagag cccagagcga
    15001 tacgcaacag cgccgcgaca gtccagcaca tcaataacgg cgaaagcgta gttccggtca
    15061 ttaagtgtga aatatactgc ccgacaccgc tatctactaa tacctgctta aacgcgccgc
    15121 cgccagcgat aataaacaca atcatcgcga tagcgcctat agaatcccca atgatatcca
    15181 tgatttgctc gatggtgcgt ccattacgtc ggcccagtgt gaaaatcgca ataacaatgg
    15241 caataaacag cgcaacggca gggttaccga caaattcaaa gaagaggcgc acggtgttag
    15301 ttttcggtaa cgtaatttca caaacggcgg cgatagccat caggatgacc gggatcacgg
    15361 cagcgaaaat actgttccag aaggagggca tctcctcttc gctaaacaga tgaggattaa
    15421 ataagccttc cggtggtgct ttctcaaagc gagttagcag tttagaaaac agcggtcctg
    15481 cgacaataac tgtcggaatg gtaatgataa atccatatag tagagtcgtt ccgagattag
    15541 cctcaaagat agtcgcgatg gcagtaggcc ctggatgtgg cggcagaaaa cagtgggtta
    15601 cagagagcgc tgctaccatc ggtacgccaa catacaacag gggtaatcca gatgatgcca
    15661 cgatggtaaa taccaacggc agcagcagga caaaacccac ttcaaaaaac atggcgaggc
    15721 cgacaaccag accggtgatc actagcgccc attgcacgcg ttttttacca aaagtagcaa
    15781 tcagcgtagt ggcgatacgt tgtgccgcgc ccgtatcaga aatcagcttg cctaacatgg
    15841 ccccgaaacc gaggatcatt gccagcccgc cgagcgtgct gccgatacca ttttgtatag
    15901 agtgcaggac ggcctgtgca tccatccctt cggcaaatcc gacgacggca gctaccagaa
    15961 cgagggcaat aaagccgtta actttaaagc cgatcatcag gataagaagc agcgcgacgc
    16021 ctgccgcaat aatgattaat ggcatagtct ttatctctta ttagtgccag aaaagcagcg
    16081 ctttcgcgct gcacgtaggg tggtggctgt taaacagcca ctaacatgcc gccatcaaca
    16141 aacaacaggt ggccgtttac gaaatcagag gcttttgaag aaaggaacac cgcagcacca
    16201 atcagttcct gcggatcgcc ccagcgtgcg gcgggggtcc gtttgcacaa ccaggcggtg
    16261 aaggcttcgt cctcaaccag tgctttagtc atttctgttt tgaaatagcc cggcgcaata
    16321 ccgttgacct gaatattgtg gcgcgccagc tcgacgcaca tgccgcgggt gagcattttt
    16381 accgctcctt tcgatgcggc ataaggggtg atggtgtcac gtcccagttc gctttgcatc
    16441 gagcaaatat taataacttt acccgccttg cgttcaacca tgtgacgagt taccgcttgc
    16501 gacaccagga acacggcggt ctggtttact gcgatcacat cattccactc ttgttcaggg
    16561 aactcagtaa aaggatgacg gcgctggata ccggcgttat tcaccagcac atcaatgggg
    16621 ccgatgtcct tttcgatatg ttcaacggcg gcatcaattt catgtttatg agtaacatta
    16681 aaaggtgcgg caacggcctg aataccctcc tggtggagtt tttctacagc aagttctgcg
    16741 cgttcggcag taatatcatt aataattatt tgtgcgccat atttacccag gccggttgcc
    16801 agtaaatagc caatgccctg tgctgaaccg gtaatcaaga tattttttcc tgccagtgaa
    16861 aatagatcgt tcattgtatt attcctttat ttatctctta gaaaacaagc tggacttttg
    16921 ctgcctgggt tttatcaccg gcgaaacgta gcgcctcttc caggtcagtg aagagatatt
    16981 cagcactcag taaaggcagt ggattgataa cgccattcgc cagccatgac actgcggtat
    17041 taaattcgct ggtaaaacgg aaagagcctt tgagtgaaat ctccttacca atcaacgtca
    17101 tcattgggaa ttctgccatc gcgcctccca tccctacctg caccattacg ccgcgtgcac
    17161 gagtgacctc cagacaggta ttcactgatg aaggatgacc ggacacttca aagctgacat
    17221 cgaaatggcc tttttccgct ttccagtgat ccatgtcgtc gttttgtggg tttacgagca
    17281 catccgcacc catctctttg gccagcgaaa gggaacgggg actcacatcg gcacagacaa
    17341 tttccgcggc ccccagtgtt ttcactgcac tgacaatcag gcagccaatg ggcccaacac
    17401 cggaaacaaa tactcgcttg ccctgtaact cgccggcctg atgtgcggca tgaatcgcga
    17461 cggctaaagg ttcggcaaaa gccataacct tttcgtcagc tttggccgga taagggacac
    17521 attgcgacgt ttcgaccatt ttataacggg taaaaccacc atcaacatga gggaaataca
    17581 tggcactgcc aaaaaaacgc atatctgtac actgattctc gttatgttca atgcagtatt
    17641 tgcagtgacc gcacggttta gacggattaa tggctaccgt ttgcccttca tgtaattctg
    17701 atgagtcgct atgaataact ttaccgataa cttcatgacc taacaccatc ggtgccttta
    17761 tcatgaaatt acctactttt ccttcctgat aataatgtaa atcggaaccg caaattccac
    17821 ctcgggttat ttgtactaat gttccattat tattccaatc tatcgtctgc tcggtaacgg
    17881 caacagtttt cttgcccgca acaacgcagg actgtgtttt cacttgcata aattcctcac
    17941 tggtcaggta gacacctcgg aagcatttaa gcggttttaa ttgtcattat ttgtgatgaa
    18001 gatcacgtca gaaaattgtt acattactat gttacgcata acgtgatgtg ccttgtaatt
    18061 cttatcaaca gaaataaaaa aacgtgaaat tattatgccg ccaggcgtag tatcgccgca
    18121 ggtaagatga ttcaggagat tttaaatggc gggtgaaagc tttattttga tgggcgtatc
    18181 agggagtggt aaaacattaa ttggtagcaa ggttgccgcg ttattgtctg cgaaatttat
    18241 tgatggtgac gatcttcatc cagccaaaaa tatagataaa atgtcgcaag gcattccatt
    18301 atctgatgaa gatcgacttc cctggctgga acgcttaaat gatgcttcat acagtcttta
    18361 taaaaagaat gaaacaggat ttattgtctg ttcatcatta aaaaaacagt atcgtgatat
    18421 tttacgcaag ggtagccccc atgttcattt cctctggtta gatggcgact atgaaactat
    18481 tctcgcgcga atgcagcgtc gggctgggca ttttatgccg gtagcgttac taaaaagtca
    18541 gtttgaggca ctggagcgtc cacaagaaga tgagcaggat attgtgcgca ttgatatcaa
    18601 ccatgatatt gccaatgtca ccgaacaatg tcggcaggct gtgctggcga tacgacaaaa
    18661 cagaatatgt gcgaaagagg gcagcgcctc agatcagcgc tgcgaatgat tttcaaaaat
    18721 ctgctttcaa caccacgcgg tagcgcgctt taccatcgcg cacatgctgg atggcgtcgt
    18781 taattttcga catcgggaac agttcggtag tcggcgcaac cttgctgcgg gcggcaaagc
    18841 gcatcagctt acgcaactca taaggtgtac cggttgctga accagagacg ctgcggtcgc
    18901 ccgcaattaa cgtaaaggcc ggaacagaca gcggcgtgag gaccgcaccg accgtatgga
    18961 aattaccgcc ataggtcagc gcctcaaaat agggctgcca gtcgaggctg acgttgacgg
    19021 tgttgataat gagatcaaac tgccccgcca gtgctttcag tgcctgtgga tcgcggctat
    19081 tcaccacttt atcggcaccc atcgccagca cttcctgctc tttcgccgga ttagaactaa
    19141 aggctgtcac ctcgcatccc attgcgtgca gaagttttat agctatatgc cccagcccgc
    19201 caataccaat taccccaacg cggctggtgg cagtgatatg gtgcatcaac agcggtttaa
    19261 agaccgtgat accgccgcac aacagcggtc cggcagattc aatgtcgata ttttctggca
    19321 gtggaatcac ccattgccag tccgcacgca acttctcggc aaagccaccg cgattcataa
    19381 tcgtcggcac cgcaccttgc tcgcagttga tctgattacc gctaatacag gcatcgcaat
    19441 gcccgcagct gcgtgccgtc cagccaatcc cgacatgctg accgacctgc aaacctttat
    19501 cctgcgcggc actcccgagc gccactacgc gaccaatcac ctcatgcccg gcaaccagtg
    19561 gatattgtga aaatccccat tcgttatcga tcattgacag atcggaatga cagatcccgc
    19621 agttatccac ctgcacttca acatcttgtg gcttcagttc accgggatcg tactcataaa
    19681 cttccagttc gccacccgct tcttttgcgg catagctttt tatcatcgac attttttggt
    19741 ccttctctgg tgtggtctgg gaagtgtaga gcatggcagg gcggggtgcc tggagtgtga
    19801 caaaggttac acatcgctgt atgcaatgct gaaattttca gcacttagcg aggctggcac
    19861 aagctggcgc ttgcatggtg gcgcgtgaca ggtataattc acaacgtttt ccgcatacct
    19921 cttcagtgcc gaagtggcga aatcggtaga cgcagttgat tcaaaatcaa ccgtagaaat
    19981 acgtgccggt tcgagtccgg ccttcggcac caaaagtatg taaatagacc tcaactgagg
    20041 tcttttttta tgcctgaaat ccgcgccacg caaggctttt ccagcttttc tcctcaactg
    20101 aagtcaacct gactcaaccc acatcaacag gcttgtgagt atactaatga gtatatttgc
    20161 cgattcgata ttgcttgtat actcacgaga taccaatgga aggaagacca ttatggccct
    20221 aacggatact aaagttcgct ctgcgaaacc tgaggagaaa gagtattcac ttgttgacgg
    20281 cgacggcatg tccttacttg taaaacctgg cggttcaaag tattggcgat ttcgtttccg
    20341 ttttggcggt aaacaacatt tgatggcgtt cggcgtttac ccggatgttt cactggcgga
    20401 tgcgcggaag aaaagggaag aggccagaaa gctggtagct gctggtatcg atccacgcga
    20461 acataagcgt gctgtgaaag aagagcaggc gaaagagatt attactttcg agaaggttgc
    20521 cagagagtgg ctcgtaacta accaaaaatg gtcggaagat catgctaatc gtgtaaaaaa
    20581 gagcctggag gacaatatct tcccggcaat tggttctcgc aatattgctg aactgggcac
    20641 ccgcgatctg ttaattccta ttaaagccgt ggagaaatcc ggatgtcttg aagtggcttc
    20701 ccgccttcaa cagcgcacca cggccatcat gcgttatgca gtgcaaagcg ggttaattga
    20761 ttacaatccg gcgcaagaga tggcgggggc ggttgcttct agtaatcgac agcatcgtcc
    20821 ggcgctggag ttaaagcgta tcccggaatt actccagaaa atcgatgaat acaccggcag
    20881 gccactaacc cgctgggcaa ccgaacttac tctgcttatt tttattcgat ccagtgagct
    20941 gcgttttgct cgctggtcag aaatcgattt tgaaacgtca atgtggacca tcccgcccga
    21001 gcgagagcct attcccggcg tgaaacattc tcagcgagga tcaaagatgc gtacaccgca
    21061 tctggtgcca ctttcaaagc aggcgctggc gatcctgaag cagataaaac agttctgtgg
    21121 cgaacatgag ctgattttta ttggcgatca cgatccgcgt aagcccatga gcgagaacac
    21181 ggtaaacagt gcactacggg tgatgggtta tgacactaag gttgaagttt gcggccacgg
    21241 cttccgtaca atggcctgta gttcgttaat tgagtcagga ctatggtcga aggatgcggt
    21301 ggaacgacag atgagtcata tggaacgcaa ttcggtgagg gcggcgtata tccataaagc
    21361 ggaacatctg gatgagcgca agctgatgtt gcagtggtgg gcggattttt tggatgccaa
    21421 tcgggagaag gggattagtc cgtttgattt tgctaagcaa gcagcaagat aaactcttca
    21481 acaccagtaa tctattgaaa aatagatatt ttaatatttt tcttggtatc ttc
//
LOCUS       DADSYM010000076        21516 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2415
            SAMN08039579-rid11723173.denovo.076, whole genome shotgun sequence.
ACCESSION   DADSYM010000076 DADSYM010000000
VERSION     DADSYM010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039579
            Sequence Read Archive: SRR6362533
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 21516)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21516)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980162.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 03:39:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,484
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 4,347
            CDSs (with protein)               :: 4,347
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 43 of 85
            Pseudo Genes (incomplete)         :: 37 of 85
            Pseudo Genes (internal stop)      :: 19 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21516
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039579-rid11723173.denovo.076"
                     /strain="LGE2415"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8302 N 95.9628 W"
                     /collection_date="2015-08-24"
     gene            305..1204
                     /gene="yegS"
                     /locus_tag="J1Y83_002494"
     CDS             305..1204
                     /gene="yegS"
                     /locus_tag="J1Y83_002494"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid kinase YegS"
                     /protein_id="HBA7630636.1"
                     /translation="MAEFPASLLILNGKSTDNLSLREAIMLLREEGMTIHVRVTWEKG
                     DAVRFVEEARKLGVATVIAGGGDGTINEVSTALIQCEGDDIPALGILPLGTANDFATS
                     VGIPEALDKALKLAIAGNAIAIDMAQVNKQTCFINMATGGFGTRITTETPEKLKAALG
                     GVSYIIHGLMRMDTLQPDRCEIRGENFHWQGDALVIGIGNGRQAGGGQQLCPNALIND
                     GLLQLRIFTGDEIIPTLVSTLKSDEDNPNIIEGASSWFDIQAPHEITFNLDGEPLSGQ
                     NFHIEILPAALRCRLPPDCPLLR"
     gene            complement(1286..2065)
                     /locus_tag="J1Y83_002495"
     CDS             complement(1286..2065)
                     /locus_tag="J1Y83_002495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HBA7630637.1"
                     /translation="MTMNSFERRNKIIQLVNEQGTVLVQDLAGVFAASEATIRADLRF
                     LEQKGVVTRFHGGAAKIMSGNSETETQEVGFKERFQLASAPKNRIAQAAVKMIHEGMT
                     VILDSGSTTMLIAEGLMTAKNITVITNSLPAAFALSENKDITLVVCGGTVRHKTRSMH
                     GSIAERSLQDINADLMFVGADGIDAVNGITTFNEGYSVSGAMVTAANKVIAVLDSSKF
                     NRRGFNQVLPIEKIDIIITDDAVSEVDKLALQKTRVKLITV"
     gene            complement(2165..3205)
                     /gene="gatD"
                     /locus_tag="J1Y83_002496"
     CDS             complement(2165..3205)
                     /gene="gatD"
                     /locus_tag="J1Y83_002496"
                     /EC_number="1.1.1.251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HBA7630638.1"
                     /translation="MKSVVNDTDGIVRVAESVIPEIKHQDEVRVKIASSGLCGSDLPR
                     IFKNGAHYYPITLGHEFSGYIDAVGSGVDDLHPGDAVACVPLLPCFTCPECLKGFYSQ
                     CAKYDFIGSRRDGGFAEYIVVKRNNVFALPADMPIEDGAFIEPITVGLHAFHLAQGCE
                     NKNVIIIGAGTIGLLAIQCAVALGAKSVTAIDISSEKLALAKSFGAMQTFNSREMSAP
                     QIQGVLRELRFNQLILETAGVPQTVELAVEIAGPHAQLALVGTLHQDLHLTSATFGKI
                     LRKELTVIGSWMNYSSPWPGQEWETASRLLTERKLSLEPLIAHRGSFESFAQAVRDIA
                     RNAMPGKVLLIP"
     gene            complement(3253..4608)
                     /locus_tag="J1Y83_002497"
     CDS             complement(3253..4608)
                     /locus_tag="J1Y83_002497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HBA7630639.1"
                     /translation="MFSEVMRYILDLGPTVMLPIVIIMFSKILGMKAGDCFKAGLHIG
                     IGFVGIGLVIGLMLDSIGPAAKAMAENFDLNLHVVDVGWPGSSPMTWASQIALVAIPI
                     AILVNVAMLLTRMTRVVNVDIWNIWHMTFTGALLHLATGSWMIGMAGVVIHAAFVYKL
                     GDWFARDTRNFFELEGIAIPHGTSAYMGPIAVLVDAIIEKIPGVNRIKFSADDIQRKF
                     GPFGEPVTVGFVMGLIIGILAGYDVKGVLQLAVKTAAVMLLMPRVIKPIMDGLTPIAK
                     QARSRLQAKFGGQEFLIGLDPALLLGHTAVVSASLIFIPLTILIAVCVPGNQVLPFGD
                     LATIGFFVAMAVAVHRGNLFRTLISGVIIMSITLWIATQTIGLHTQLAANAGALKAGG
                     MVASMDQGGSPITWLLIQVFSPQNIPGFIVIGAIYLTGIFMTWRRARGFIKQEKAVLA
                     E"
     gene            complement(4612..4896)
                     /gene="gatB"
                     /locus_tag="J1Y83_002498"
     CDS             complement(4612..4896)
                     /gene="gatB"
                     /locus_tag="J1Y83_002498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIB"
                     /protein_id="HBA7630640.1"
                     /translation="MKRKIIVACGGAVATSTMAAEEIKELCQSHNIPVELIQCRVNEI
                     ETYMDGVHLICTTARVDRSFGDIPLVHGMPFVSGVGIEALQNKILTILQG"
     gene            complement(4927..5379)
                     /gene="gatA"
                     /locus_tag="J1Y83_002499"
     CDS             complement(4927..5379)
                     /gene="gatA"
                     /locus_tag="J1Y83_002499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIA"
                     /protein_id="HBA7630641.1"
                     /translation="MTNLFVRSGISFVDRSEVLTHIGNEMLAKGVVHDTWPQALIARE
                     AEFPTGIMLEQHAIAIPHCEAIHAKSSAIYLLRPTNKVHFQQADDDNDVAVSLVIALI
                     VENPQQQLKLLRCLFGKLQQPDIVETLITLPETQLKEYFTKYVLDSDE"
     gene            complement(5389..6651)
                     /gene="gatZ"
                     /locus_tag="J1Y83_002500"
     CDS             complement(5389..6651)
                     /gene="gatZ"
                     /locus_tag="J1Y83_002500"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatZ"
                     /protein_id="HBA7630642.1"
                     /translation="MKTLIARHKAGEHIGICSVCSAHPLVIEAALAFDRNSTRKVLIE
                     ATSNQVNQFGGYTGMTPADFREFVFAIADKVGFARERIILGGDHLGPNCWQQENADAA
                     MEKSVELVKAYVRAGFSKIHLDASMSCADDSIPLAPETVAERAAVLCLAAESVATDCQ
                     REQLNYVIGTEVPVPGGEASAIQSVHITQVEDAANTLRTHQKAFIARGLAEALTRVIA
                     IVVQPGVEFDHSNIIHYQAQEAQALAQWIEKTKMVYEAHSTDYQTQTAYRELVRDHFA
                     ILKVGPALTFALREAIFALAQIEQELIAPENRSRCLAVIEEVMLDEPQYWKKYYRTGF
                     NDSLLDIRYSLSDRIRYYWPHSRIKNSVETMMVNLEGVDIPLGMISQYLPKQFERIQS
                     GELSAIPHQLIMDKIYDVLRAYRYGCAE"
     gene            complement(6680..7534)
                     /gene="gatY"
                     /locus_tag="J1Y83_002501"
     CDS             complement(6680..7534)
                     /gene="gatY"
                     /locus_tag="J1Y83_002501"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416599.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatY"
                     /protein_id="HBA7630643.1"
                     /translation="MYVVSTKQMLNNARRGGYAVPAFNIHNLETMQVVVETAASMHAP
                     VIIAGTPGTFTHAGTENLMALVSAMAKQYHHPLVIHLDHHTKFDDIAQKVRSGVRSVM
                     IDASHLPFAQNISRVKEVVDFCHRFDVSVEAELGQLGGQEDDVQVNEADAFYTNPVQA
                     REFAEATGIDSLAVAIGTAHGMYARAPALDFSRLENIRQWVNLPLVLHGASGLSTKDI
                     QQTIKLGICKINVATELKNAFSQALKNYLTEHPEATDPRDYLQSAKSAMRDVVSKVIA
                     DCGCEGRA"
     gene            complement(7763..8815)
                     /gene="fbaB"
                     /locus_tag="J1Y83_002502"
     CDS             complement(7763..8815)
                     /gene="fbaB"
                     /locus_tag="J1Y83_002502"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006820796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fructose-bisphosphate aldolase"
                     /protein_id="HBA7630644.1"
                     /translation="MTDIAQLLGKDADNLLQHRCMTIPSDQLYLPGHDYVDRVMIDNN
                     RPPAVLRNMQTLYNTGRLAGTGYLSILPVDQGVEHSAGASFAANPLYFDPKNIVELAI
                     EAGCNCVASTYGVLASVSRRYAHRIPFLVKLNHNETLSYPNTYDQTLYASVEQAFNMG
                     AVAVGATIYFGSEESRRQIEEISAAFERAHELGMVTVLWAYLRNSAFKKDGVDYHVSA
                     DLTGQANHLAATIGADIVKQKMAENNGGYKAINYGYTDDRVYSKLTSENPIDLVRYQL
                     ANCYMGRAGLINSGGAAGGETDLSDAVRTAVINKRAGGMGLILGRKAFKKSMADGVKL
                     INAVQDVYLDSKITIA"
     gene            9072..10349
                     /locus_tag="J1Y83_002503"
     CDS             9072..10349
                     /locus_tag="J1Y83_002503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBA7630645.1"
                     /translation="MKTTAKLSFMMFVEWFIWGAWFVPLWLWLSKSGFSAGEIGWSYA
                     CTAIAAILSPILVGSITDRFFSAQKVLAVLMFAGAVLMYFAAQQTTFAGFFPLLLAYS
                     LTYMPTIALTNSIAFANVPDVERDFPRIRVMGTIGWIASGLACGFLPQMLGYADISPT
                     NIPLLITAGSSALLGVFAFFLPDTPPKSTGKMDIKVMLGLDALILLRDKNFLVFFFCS
                     FLFAMPLAFYYIFANGYLTEVGMKNATGWMTLGQFSEIFFMLALPFFTKRFGIKKVLL
                     LGLVTAAIRYGFFIYGSADEYFTYALLFLGILLHGVSYDFYYVTAYIYVDKKAPVHMR
                     TAAQGLITLCCQGFGSLLGYRLGGVMMEKMFAYQEPVNGLTFNWAGMWTFGAVMIAII
                     AVLFMIFFRESDNEITAIKVDDRDIALTQGEVK"
     gene            10346..11350
                     /locus_tag="J1Y83_002504"
     CDS             10346..11350
                     /locus_tag="J1Y83_002504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HBA7630646.1"
                     /translation="MKTERILGALYGQALGDAMGMPSELWPRSRVKAHFGWIDRFLPG
                     PKENNAACYFNRAEFTDDTSMALCLADALLEREGKIDPDLIGRNILGWALRFDAFNKN
                     VLGPTSKIALNAIRDGKPVAELENNGVTNGAAMRVSPLGCLLPARDVDSFIDDVALAS
                     SPTHKSDQAVAGAVVIAWAISRAIDGESWSAIVDSLPSIARHAQQKRITTFSASLAAR
                     LEIALKIVRNADGTESASEQLYQVVGAGTSTIESVPCAIALVELAQTDPNRCAVLCAN
                     LGGDTDTIGAMATAICGALHGVNAIDPALKAELDAVNQLDFNRYATALAKYRQQREAI
                     "
     gene            11347..12312
                     /locus_tag="J1Y83_002505"
     CDS             11347..12312
                     /locus_tag="J1Y83_002505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HBA7630647.1"
                     /translation="MSGARLHTLLPELATRQPVMVVGAAVIDVIADAYALPWRGCDIE
                     LKQQSVNVGGCALNIAVALKRLGIEAGNALPLGQGVWAEIIRNRMAKEDLISLIDNAE
                     GDNGWCLALVEPDGERTFMSFSGVENQWNRQWLARLTVAPGSLLYFSGYQLASPCGEL
                     LVEWLEKLQDVTPFIDFGPRIGDIPDALLARIMACRPLVSLNRQEAEIAAERFALSAE
                     ITTLGKQWQEKFAAPLIVRLDKEGAWYFSNDASGCIPAFPTQVVDTIGAGDSHAGGVL
                     AGLASGLPLADAVLLGNAVASWVVGHRGGDCAPTREELLLAHKNV"
     gene            complement(12286..13032)
                     /locus_tag="J1Y83_002506"
     CDS             complement(12286..13032)
                     /locus_tag="J1Y83_002506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBA7630648.1"
                     /translation="MEQAHTQLIAQLNERILAADNTPLYIKFAETVKNAVRSGVLEHG
                     NILPGERDLSQLTGVSRITVRKAMQALEEEGVVTRSRGYGTQINNIFEYSLKEARGFS
                     QQVVLRGKKPDTLWVNKRVVKCPEEVAQQLAVEAGSDVFLLKRIRYVDEEAVSIEESW
                     VPAHLIHDVDAIGISLYDYFRSQHIYPQRTRSRVSARMPDAEFQSHIQLDSKIPVLVI
                     KQVALDQQQRPIEYSISHCRSDLYVFVCEE"
     gene            complement(13084..13902)
                     /locus_tag="J1Y83_002507"
     CDS             complement(13084..13902)
                     /locus_tag="J1Y83_002507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416605.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 25 protein"
                     /protein_id="HBA7630649.1"
                     /translation="MQLRITSRKKFTVLLCALGLISIVAIYPRQTVNFFYSTAIQIKD
                     YIHFYGYRPVKSFAIRIPASYTIHGIDVSRWQERIDWQRVAKMRDNGIRLQFAFIKAT
                     EGEKLVDPYFSRNWQLSRENGLLRGAYHYFSPSVSASVQARLFLQTVDFSQGDFPAVL
                     DVEERGKLSAKELRKRVSQWLKMVEKRTGKKPIIYSGAVFYHANLAGYFTEYPWWVAH
                     YYQRRPDNDGMAWRFWQHSDRGQVDGINGPVDFNVFNGTVEELQAFVDGIKETP"
     gene            complement(13967..14767)
                     /gene="thiD"
                     /locus_tag="J1Y83_002508"
     CDS             complement(13967..14767)
                     /gene="thiD"
                     /locus_tag="J1Y83_002508"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HBA7630650.1"
                     /translation="MKRINALTIAGTDPSGGAGIQADLKTFSALGAYGCSVITALVAQ
                     NTRGVQSVYRIEPDFVAAQLDSVFSDVRIDTTKIGMLAETDIVEAVAERLQRYQIQNV
                     VLDTVMLAKSGDPLLSPSAVATLRSRLLPQVSLITPNLPEAAALLDAPHARTEQEMLE
                     QGRSLLAMGCGAVLMKGGHLDDEQSPDWLFTREGEQRFTAQRIMTKNTHGTGCTLSAA
                     LAALRPRHTNWADTVQEAKSWLSSALAQADTLEVGHGIGPVHHFHAWW"
     gene            complement(14764..15552)
                     /gene="thiM"
                     /locus_tag="J1Y83_002509"
     CDS             complement(14764..15552)
                     /gene="thiM"
                     /locus_tag="J1Y83_002509"
                     /EC_number="2.7.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyethylthiazole kinase"
                     /protein_id="HBA7630651.1"
                     /translation="MQVDLLSSAQSAHALHLFHQHSPLVHCMTNDVVQTFTANTLLAL
                     GASPAMVIETEEASQFAAIASALLINVGTLTQPRAQAMSAAVEQATRSQTPWTLDPVA
                     VGALDYRRRFCVELLSHKPTAIRGNASEIMALAGVANGGRGVDTTDAAANAIPAAQTL
                     ARETGAIVVVTGEVDYVTDGHRIVGIHGGDPLMTKVVGTGCALSAVVAACCALPGDTL
                     ENVASACHWMKQAGERAVARSEGPGSFVPHFLDALWQLAQEVQA"
     regulatory      complement(15587..15683)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(15775..16047)
                     /gene="rcnR"
                     /locus_tag="J1Y83_002510"
     CDS             complement(15775..16047)
                     /gene="rcnR"
                     /locus_tag="J1Y83_002510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni(II)/Co(II)-binding transcriptional repressor
                     RcnR"
                     /protein_id="HBA7630652.1"
                     /translation="MSHTIRDKQKLKARASKIQGQVVALKKMLDEPHECAAVLQQIAA
                     IRGAVNGLMREVIKGHLTEHIVHQGDELKREEDLDVVLKVLDSYIK"
     gene            16168..16998
                     /locus_tag="J1Y83_002511"
     CDS             16168..16998
                     /locus_tag="J1Y83_002511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel/cobalt efflux protein RcnA"
                     /protein_id="HBA7630653.1"
                     /translation="MTEFTTLLQQGNAWFFIPSAILLGALHGLEPGHSKTMMAAFIIA
                     IKGTIKQAVMLGLAATISHTAVVWLIAFGGMVISKRFTAQSAEPWLQLISAVIIISTA
                     FWMFWRTWLGERNWLENMHEHDHEHEHHHHDHEDHHDHGHHHHHEHGEYQDAHARAHA
                     NDIKRRFDGREVTNWQILLFGLTGGLIPCPAAITVLLICIQLKALTLGATLVVSFSTG
                     LALTLVTVGVGAAISVQQVAKRWSGFNTLAKRAPYFSSLLIGLVGVYMGVHGFMGIMR
                     "
     gene            17217..17555
                     /gene="rcnB"
                     /locus_tag="J1Y83_002512"
     CDS             17217..17555
                     /gene="rcnB"
                     /locus_tag="J1Y83_002512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416610.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni(II)/Co(II) efflux transporter accessory
                     subunit RcnB"
                     /protein_id="HBA7630654.1"
                     /translation="MTIKNKMLLGALLLVTSAAWAAPATAGSTNTSGISKYELSSFIA
                     DFKHFKPGDTVPEMYRTDEYNIKQWQLRNLPAPDAGTHWTYMGGAYVLISDTDGKIIK
                     AYDGEIFYHR"
     gene            complement(17638..18672)
                     /locus_tag="J1Y83_002513"
     CDS             complement(17638..18672)
                     /locus_tag="J1Y83_002513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative fimbrial-like adhesin protein"
                     /protein_id="HBA7630655.1"
                     /translation="MEIRIMLFIVMMMVMPMSYAACYSELSVQHNLVVQGDFALSQTQ
                     IATYEHNFNDSSCVSTNTIIPMSPSDIIVGLYDDTIKLNLHFEWTNKNNITLSNNQTS
                     FTSGYSVTVTPAASNAKVNVSAGGGGSVMINGVATLSSASSSTRGSAAVQFLLCLLGG
                     KSWDACVNSYRNALAQNAGVYSFNLTLSYNPITTTCKPDDLLITLESIPVSQLPATGN
                     KTTINSKKGDIILRCKNLLGQQNQTSRKMQVYLSSSDLLTNSNTILKGAEDNGVGFIL
                     ESNGTPVTLLNITNSSKGYTNLKEIAAKSKLIDTTVSIPITASYYVYDTNKVKSGALE
                     ATALINVKYD"
     gene            complement(18688..21168)
                     /locus_tag="J1Y83_002514"
     CDS             complement(18688..21168)
                     /locus_tag="J1Y83_002514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HBA7630656.1"
                     /translation="MLRMTPLASAIVALLIGIEAYAAEETFDTHFMIGGMKDQQVSNI
                     RLEDNQPLPGQYDIDIYVNKQWRGKYEIVVKDNPQETCLSREMIKRLGINTDNFASGK
                     QCLTFKQLIQGGSYTWDIGVFRLDFSVPQAWVEELESGYVPPENWERGINAFYTSYYV
                     SQYYSDYKASGNSKSTYVRFNSGLNLQEWQLHSDASFSKTNNNPGVWKSNTLYLERGF
                     AQLLGTLRVGDMYTSSDIFDSVRFSGVRLFRDMQMLPNSKQNFTPRVQGIAQSNALVT
                     IEQNGFVVYQKEVPPGPFAITDLQLAGGGADLDVSVKEADGSVTTYLVPYAAVPNMLQ
                     PGVSKYDFAAGRSHIEGASKQSDFVQAGHQYGFNNLLTLYGGSMVANNYYAFTLGTGW
                     NTRIGAISVDATKSHSKQDNGDVFDGQSYQIAYNKFVSQTSTRFGLAAWRYSSRDYRT
                     FNDHVWANNKDNYRHDENDIYDIADYYQNDFGRKNSFSANMSQSLPEGWGSVSLSTLW
                     RDYWGRSGSSKDYQLSYSNNLRRISYTLAASHAYDENHHEEKRFNIFISIPFDWGDDV
                     TTPRRQIYMSNSTTFDDQGVASNNTGLSGTVGSRDQFNYGVNLSYQYQGNETTAGANL
                     TWNAPVATVNGSYSQSSAYRQAGASVSGGIVAWSGGVNLANRLSETFAVMNAPGIKDA
                     YVNGQKYRTTNRNGVVVYDGMTPYRENYLMLDVSQSDSEAELRGNRKIAAPYRGAVVL
                     VNFDTDQRKPWFIKALRADGQPLTFGYEVNDIHGHNIGVVGQGSQLFIRTNEVPPSVN
                     VAIDKQQGLSCTITFGKEIDESRNYICQ"
     gene            complement(21184..>21516)
                     /locus_tag="J1Y83_002515"
     CDS             complement(21184..>21516)
                     /locus_tag="J1Y83_002515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HBA7630657.1"
                     /translation="SPEQEGKNALKFAMQNRIKLFYRPAGIAPVNKVTFKKLLVNRSG
                     NGLVIKNDSANWVTISDVKANNVKVNYETIMIAPLESQSVNVKSNNANNWHLTIIDDH
                     GNYISDKI"
BASE COUNT         5412 a         5523 c         4967 g         5614 t
ORIGIN      
        1 tacataagta ggaattacag acatggaggc ccttccgggt aaccccatgg gggactttaa
       61 tatattttga atagtatatt gaagatgcca ctgtttagtt gaatattagg tatattctct
      121 tttttaaaat ttatcagtgg ctaccgttaa tattcgctgt ccccattgta agctcctggt
      181 ggtaagcact gaatccccat acctgaactg actttttatc ctccgacttt catcctgttc
      241 tgactccacc ttttcttttc tgctctacac tatctacaaa ccaatcataa aggcacatac
      301 gatcatggca gaatttcccg ccagcttact gattcttaat ggcaaaagta ctgacaatct
      361 atccttgcgc gaagcaatta tgctgttgcg tgaggaagga atgacgatcc atgtgcgggt
      421 tacctgggag aaaggtgatg ccgtacgatt tgtagaggag gcccggaagt taggtgtcgc
      481 aacagtgatt gccggtggtg gcgatggcac cattaatgaa gtttctacgg cattgattca
      541 atgtgagggg gatgacatac ccgcgctggg aattttacca ttaggaactg ccaatgattt
      601 tgccaccagt gtagggattc ctgaggcact ggataaggcg ctgaaactgg caattgccgg
      661 taacgccatt gcgatagata tggcgcaggt caacaaacaa acctgtttta ttaatatggc
      721 gacaggcgga tttgggacgc gtattaccac agaaacgccg gaaaaattaa aagccgcgct
      781 gggtggcgtc tcttacatca ttcatggctt aatgcgcatg gacactctgc aaccggaccg
      841 ttgtgaaatc cgcggtgaaa actttcactg gcaaggtgac gccctggtca ttggtattgg
      901 taacgggcgt caggccggtg gcggtcaaca attgtgcccg aacgcattaa ttaacgatgg
      961 cttgctgcaa ctgcgcattt ttaccggcga tgaaattatt ccgactctcg tatcaacctt
     1021 aaaatctgac gaagataacc cgaatattat cgaaggtgct tcgtcgtggt ttgatataca
     1081 agccccacac gaaatcactt ttaatcttga tggcgaaccg ttgagtgggc aaaacttcca
     1141 tattgaaata cttccggcgg cgttgcgttg tcgattacca ccagattgtc cattgttgcg
     1201 ttaatcaaac tattctctgt tgaaccagag agaatccgta atgccggatg cggtgtaaac
     1261 acctcatccg gcatacagaa caatactaca ccgtaattaa ttttacccgt gtcttctgca
     1321 atgccagttt atccacctca gacacagcat catcggtaat aataatgtca attttttcga
     1381 ttggcagtac ctgattaaaa ccgcgacgat taaatttcga tgaatcaaga acggcaatca
     1441 ctttattcgc cgctgtcacc atcgcaccgc taacggaata accttcatta aaggtcgtaa
     1501 taccattaac cgcatcaata ccatcagcac cgacaaacat taaatcggca ttaatatcct
     1561 gcagggaacg ttcagcaata gatccatgca tagatcgcgt tttatggcgg accgtgccac
     1621 cgcagacgac cagagtaatg tctttatttt cggaaagggc aaacgccgcc gggagactgt
     1681 tggtaatcac cgtgatattt ttggcggtca ttaatccttc ggcgataagc attgtggtac
     1741 ttccgctgtc gagaataacg gtcatccctt cgtggatcat tttgactgcc gcctgcgcta
     1801 ttctgttttt cggcgcgctg gcgagctgaa atcgctcttt aaacccgact tcctgggtct
     1861 cggtttcgct attaccggac attattttcg ccgcaccgcc atgaaagcgc gtaaccacgc
     1921 ctttttgttc gagaaagcgc aaatcggcac ggattgtcgc ttccgaggca gcaaatactc
     1981 ccgccagatc ctgaacaagc acggttccct gttcattcac taattggatg atcttattcc
     2041 ttcgctcgaa tgaattcatc gtcatttacc catattcaat tgtggctagt gtaaacgaag
     2101 ggggaccatt ggtgaatgat ctgttttgca caataccggg cctgcatcac gctggcccgc
     2161 ggtttcaggg aatgagcaac actttgcccg gcatggcatt acgagcgatg tcacgcaccg
     2221 cctgggcgaa gctttcaaag cttccacggt gagcgattaa tggctccagg cttaacttac
     2281 gttctgtcag caaccggctc gccgtttccc actcctgccc cggccaaggg ctggagtagt
     2341 tcatccaact gccgataacc gtcagctctt tacgcaatat tttgccaaac gttgcagatg
     2401 ttaaatgcag atcctgatgc aacgtgccca ccagcgccag ttgggcatga ggaccggcaa
     2461 tctctaccgc cagttcgacg gtttgcggga cgccagccgt ctcgaggata agctgattaa
     2521 agcgcagctc gcgtaaaacg ccctgtattt gcggcgcgct catttcacgg ctgttaaatg
     2581 tttgcatcgc accgaaagat tttgccagtg ccagtttttc tgagctaatg tcgatagccg
     2641 tcacactctt tgctcccagc gcgacagcgc actgaattgc cagcaggcca atggttccgg
     2701 caccaataat aataacgttt ttattctcac atccttgcgc taaatggaac gcatgcagac
     2761 caacggtaat cggttcaata aaagcgccat cctcaatagg catatccgca ggtagtgcaa
     2821 agacattgtt tcgcttaacg acaatatatt cggcaaatcc accatcacgc cgtgagccaa
     2881 taaaatcata ttttgcacac tgggaataaa accctttcag acactctgga caagtaaaac
     2941 agggtaataa cggcacacag gcaaccgcat cgccggggtg taaatcatca acaccggatc
     3001 ccaccgcatc aatatagcca ctaaattcat ggcctaacgt tattggataa taatgtgcac
     3061 catttttaaa tatcctgggt aaatcggaac cacataagcc cgagctggca atttttaccc
     3121 gcacctcatc ctgatgttta atttcaggaa tgacgctttc tgctacgcgc acgataccat
     3181 cagtatcatt caccactgat ttcataaaaa ctcctgattg ttaaggggga taacctcccc
     3241 ctcaggtaaa aattattctg cgagaacggc tttctcttgt ttaataaaac cacgcgctct
     3301 acgccaggtc atgaaaatac cggtaagata aattgcgccg ataacaatga aaccgggaat
     3361 attttgcggg gagaaaacct gaatcagtaa ccaggtaatg ggagaaccgc cctgatccat
     3421 tgaagccacc atacccccgg cttttaacgc gcccgcatta gccgccagtt gggtgtgcaa
     3481 accaatagtt tgcgtcgcga tccacagggt gatgctcata atgatgacac ccgaaattaa
     3541 ggtgcggaac agatttccac gatgcaccgc gaccgccatc gccacaaaga agccgatggt
     3601 ggcaaggtcg ccaaacggca gcacctgatt acccggcaca caaacagcaa ttaaaatggt
     3661 gagtggaata aaaatcaggc ttgccgatac caccgccgta tgtcccagca gcaacgccgg
     3721 atcaaggcca atcaggaact cctgaccgcc gaacttcgcc tgtaaacgac tacgggcctg
     3781 cttagcgatg ggcgttaaac catccatgat gggtttaatc acccgtggca tcagcagcat
     3841 aactgccgcc gtttttaccg ccagctgcaa tacacctttg acatcgtaac ccgcgaggat
     3901 gccgataatc agccccatca caaaacccac ggtgaccggt tcgccaaaag ggccaaattt
     3961 gcgctgaata tcatcggcgc taaatttaat tcggttaacg cctgggattt tctcgatgat
     4021 agcatcgacc agcaccgcaa tcggccccat atacgccgac gtaccgtgcg gaatagcaat
     4081 gccttccagc tcaaagaaat ttcgggtatc gcgggcaaac cagtcgccga gcttataaac
     4141 aaacgccgcg tgaattacca cacccgccat ccctatcatc catgaaccgg ttgccagatg
     4201 cagcaacgcg ccggtgaagg tcatatgcca gatattccag atatcaacat ttaccacccg
     4261 cgtcatacgg gtcagtaaca tcgccacgtt aaccagaatc gcaatcggaa tcgccaccag
     4321 cgcaatttgc gacgcccagg tcatgggtga agagcccggc cagccgacat cgaccacatg
     4381 cagattcagg tcgaaatttt ccgccatcgc tttcgccgcc gggccaatgg aatccagcat
     4441 taagccaatc acaaggccaa tgccaacaaa gccaatcccg atatgcagac ctgctttaaa
     4501 gcaatcgcct gccttcatgc ctaatatttt agaaaacata atgatgacaa tcggcagcat
     4561 caccgtaggg ccgaggtcga gaatataacg catgacttct gaaaacatag gtcacccctg
     4621 taagatagtc agaattttat tttgtaatgc ttcgataccg acaccagaaa caaaaggcat
     4681 gccgtgaact aacggaatat cgccaaaact acgatccacc ctggcagtgg tgcatatcaa
     4741 atgcacgcca tccatatagg tttctatttc attaacccga cactggatta attcaacagg
     4801 aatattatga ctctgacaca actctttaat ttcttccgcc gccatcgtag aggtcgcaac
     4861 cgcgcctccg caagcgacaa taattttgcg tttcataaat agtccttttt attgttacag
     4921 agggatttat tcgtctgaat ctaaaacata ctttgtgaag tattccttta actgggtttc
     4981 aggaagagtg attagtgtct cgacgatatc aggctgttgt aacttgccaa ataaacagcg
     5041 taaaagtttc aattgctgct gcggattttc cacaatcaac gcaataacca acgataccgc
     5101 cacgtcgtta tcatcatccg cttgctgaaa atgaacttta tttgttggcc ttaacagata
     5161 aatggctgac gacttagcat gaatcgcctc acaatgcggt attgcaatgg cgtgctgctc
     5221 aagcattatc ccggtaggga attctgcttc tctggcaatt aatgcctgtg gccaggtatc
     5281 atgaaccaca cctttcgcga gcatctcatt accgatatgg gttaaaactt cgctacgatc
     5341 gacaaaagaa attccgctac gaacaaacag gttagtcata taccgtcctt attccgcaca
     5401 gccgtagcga taggcgcgca aaacatcata aattttatcc ataataagct gatgcggtat
     5461 tgctgataat tccccggact gaatgcgttc aaattgtttc ggaagatact gactaatcat
     5521 gcccagtggg atgtccacgc cttccagatt caccatcatc gtttcgacgc tatttttaat
     5581 ccggctatgc ggccagtaat aacgaatacg atctgacagg ctgtaacgaa tatccagcaa
     5641 tgaatcgtta aaacccgtgc gataatattt tttccagtat tgcggttcgt cgagcatcac
     5701 ttcttcaatc accgccagac aacggctacg attttctgga gcgatgagtt cctgctcaat
     5761 ttgtgccagc gcaaatatcg cctcacgtaa agcaaaggtt aatgccggac cgactttcaa
     5821 aattgcaaaa tgatcgcgaa ctaattcccg ataggccgtc tgagtctggt aatcggtgga
     5881 atgcgcttca taaaccattt tggttttttc tatccattgc gccaacgcct gagcttcttg
     5941 cgcctgataa tgaataatat tgctatggtc aaattccaca cccggctgca ccacgatggc
     6001 aatcacgcgt gttaacgcct ctgccagacc gcgggcaata aacgcttttt gatgcgtgcg
     6061 taaagtattg gcggcatctt caacctgggt gatgtgtact gactgaatgg cgctggcctc
     6121 accgcccgga accggaactt cagtgccaat gacatagttc agttgctcac gctggcaatc
     6181 agtcgccaca ctttcagccg ccagacaaag cacagcggca cgttcagcaa ccgtttctgg
     6241 cgctaacggt atggaatcgt cagcgcagga cattgacgca tcaagatgaa ttttactgaa
     6301 gccggcacga acatatgcct ttaccagctc gacggatttt tccatcgccg catccgcatt
     6361 ttcttgctgc cagcagtttg gtcccagatg gtcgccgccg agaataatac gctcacgagc
     6421 aaatccgact ttatcggcaa tcgcaaaaac aaattcgcga aagtctgccg gtgtcattcc
     6481 ggtataaccg ccaaattgat tgacctggtt tgacgttgct tcaatcagca ctttacgcgt
     6541 gctgttgcga tcaaatgcca gcgccgcttc gataaccaac ggatgtgcag aacagactga
     6601 acatatgcca atatgttcac cagctttatg ccgggcaatt aacgttttca tgttttttcc
     6661 ttgttaaatg gcaggtgcgt tatgccctgc cctcgcagcc acaatcggca atcactttgc
     6721 tcaccacatc gcgcattgca gatttagccg actgcaaata atcacgggga tcggtcgctt
     6781 cggggtgctc ggtcaggtaa ttttttaacg cctgcgagaa agcatttttc agctccgttg
     6841 caacgttgat tttgcatatc cccagtttga tggtttgctg aatatcctta gtcgataatc
     6901 ctgacgcgcc atgcagcacc agcggtaagt tcacccactg gcgaatgttc tccagtcgag
     6961 aaaaatcaag cgccggtgct ctggcataca taccgtgagc cgtgccgatg gcgaccgcca
     7021 gggaatcaat cccggttgcc tcggcaaatt cacgcgcctg aacggggttg gtataaaaag
     7081 catcggcttc attgacttgc acatcatctt cctggccgcc aagttgcccc agctccgctt
     7141 cgacgctgac atcaaagcga tggcaaaaat ccaccacctc tttgacccgt gaaatatttt
     7201 gcgcaaaagg caaatgcgag gcgtcaatca tgactgagcg cacgccagaa cgaaccttct
     7261 gagcgatatc gtcaaatttc gtgtgatggt cgagatgaat caccagtgga tggtgatatt
     7321 gcttcgccat cgcgctgacc agcgccatca gattttctgt accggcatga gtaaatgtgc
     7381 cgggcgttcc ggcgatgatg accggcgcat gcatgctggc agcggtttcc accaccactt
     7441 gcatcgtttc gagattgtga atattgaatg ccggaaccgc ataaccgccg cgccgcgcgt
     7501 tgttcagcat ctgctttgtc gataccacgt acattttcac accctgtcat ttattttcga
     7561 tttcaaaaca taatgaaatt atttgttttt aaatgtcgcg ataacgatca caaaaacgac
     7621 aatatgaaaa tcaatccagg aatgaaaggc aaaaaaaacg gcctcccggg ggaagccgta
     7681 acaaagtacg cgtggtttta tgccggatgt ggcgttaacg ccttttccgg cctacggggt
     7741 agtgctgttc gtatgaagcg catcaggcga tagtaatttt gctatcgaga taaacgtcct
     7801 gcacggcgtt aatcagtttc acgccgtcag ccatcgattt cttgaacgcc ttacgtccaa
     7861 gaatcagccc cattccgcct gcgcgtttgt tgataaccgc agtacgcact gcatcgctga
     7921 ggtcagtttc accgcctgca gcaccgccgg agtttatcaa cccggcccgg cccatatagc
     7981 agttagctaa ctgataacgc accaggtcaa tcgggttttc actggtcagt ttgctgtaga
     8041 ctcgatcgtc ggtataaccg taattaatcg ctttatagcc gccgttattt tccgccattt
     8101 tttgtttgac gatatctgca cctatggttg ccgccagatg gttcgcctga ccagtcaggt
     8161 cggcggaaac atggtaatca acgccatctt tcttaaaggc ggagttacgc aaataggccc
     8221 acagcactgt caccatgccc agctcgtgcg cacgttcaaa agccgcagaa atttcttcaa
     8281 tctggcgacg tgactcttcc gagccaaaat agatagtcgc accaacggcc accgcgccca
     8341 tattgaacgc ctgctccacg ctggcataca gcgtttgatc gtaggtattc gggtaactta
     8401 gcgtctcgtt gtgattaagt ttgacgagga atggaatgcg atgcgcgtaa cgccgcgata
     8461 ccgatgccag cacgccgtaa gttgacgcca cacagttaca gcccgcttcg atcgccagtt
     8521 caacaatgtt tttcgggtca aagtagagcg ggttagcggc aaatgaagct ccggcagagt
     8581 gctcaacgcc ctggtcaacc ggcagaatag aaagatatcc tgtgccagcc agacgcccgg
     8641 tgttgtacaa cgtctgcata ttacgtaaca ccgctggcgg gcgattattg tcaatcatca
     8701 cgcggtctac gtagtcatgt ccggggagat aaagctggtc agaaggaata gtcatacaac
     8761 ggtgctgtaa aaggttgtcg gcgtctttgc caagcaactg cgcaatatct gtcattacta
     8821 tgctcccgta aattccgatt ggatatcggc tatggattgt cctggcccgc cttttgcggg
     8881 caatcataat cctggtcgtt acgagcgagt ttttccatct tttggatgtt ttttcagcgt
     8941 tttctgttgg ctcgattcat caggaaaaat gttaccgcgg tcaagttttc accacaaagg
     9001 tatttaaaag gtattactaa gtggtattgt catcgcgtac cttacattac ctgtcatgaa
     9061 ggaattaaaa gatgaaaaca acagcaaagc tgtcgttcat gatgtttgtt gaatggttta
     9121 tctggggcgc gtggtttgtg ccattgtggt tgtggttaag taaaagcggt tttagtgccg
     9181 gagaaattgg ctggtcgtat gcctgtaccg ccattgcggc gatcctgtcg ccgattctgg
     9241 ttggctccat cactgaccgc tttttctcgg cgcagaaagt gctggcggta ttgatgttcg
     9301 ctggtgcggt gctgatgtat ttcgctgcgc aacagaccac ttttgccggg ttcttcccgt
     9361 tactgctggc ctactcacta acttatatgc cgaccattgc gctgactaac agtatcgctt
     9421 ttgccaacgt gccggatgtg gagcgtgatt tcccgcgcat tcgtgtgatg ggcactatcg
     9481 gctggattgc ctctggtctg gcatgtggat tcttgccgca aatgctgggt tatgccgata
     9541 tctcaccgac gaacatcccg ctgctgatta ctgccggaag ttctgctctg cttggtgtgt
     9601 ttgcattttt cctgcccgac acgccaccaa aaagcaccgg caaaatggat attaaagtca
     9661 tgctcggcct ggatgcgctg atcctgctgc gcgataagaa cttcctcgtc tttttcttct
     9721 gttcattcct gtttgcgatg ccactggcgt tctattacat ctttgccaac ggttacctga
     9781 ccgaagttgg catgaaaaac gccactggct ggatgacgct cggccagttc tctgaaatct
     9841 tctttatgct ggcattgccg tttttcacca aacgctttgg tatcaaaaag gtattattgc
     9901 ttggtctggt caccgccgcg atccgttatg gcttctttat ttacggcagt gcggatgaat
     9961 atttcactta cgctctcttg ttcctcggca ttttgctgca cggcgtaagt tacgattttt
    10021 actacgttac cgcttacatc tatgtcgata aaaaagcccc cgtgcatatg cgtactgccg
    10081 cgcaggggct gatcacgctc tgctgccagg gcttcggcag tttgctcggc tatcgtcttg
    10141 gcggtgtgat gatggaaaag atgttcgctt atcaggaacc ggtaaacgga ctgactttca
    10201 actgggccgg gatgtggact ttcggcgcgg tgatgattgc cattatcgcc gtgctgttca
    10261 tgattttttt ccgcgaatcc gacaacgaaa ttacggctat caaagtcgat gatcgcgata
    10321 ttgcgttgac acaaggggaa gttaaatgaa aacagaacgt attctcggtg ctctttatgg
    10381 gcaggcgtta ggggatgcga tggggatgcc ctccgagctt tggccgcgca gtcgcgtcaa
    10441 agcacacttt ggctggattg accgttttct gcctgggcca aaggagaata acgcggcctg
    10501 ttattttaac cgcgccgaat tcaccgacga tacctcgatg gcgctgtgtc tggcggatgc
    10561 gttactggaa cgtgaaggca agatcgatcc ggatctgatt gggcgtaata ttctcggctg
    10621 ggcgctgcgt ttcgacgcct ttaacaaaaa cgtactaggt ccgacctcga agattgcgct
    10681 taacgccatt cgcgacggta aacccgttgc ggaactggaa aataacggcg tgaccaacgg
    10741 cgcagcgatg cgcgtctcgc cattaggttg tttgcttccg gcgcgcgatg ttgattcctt
    10801 tattgatgat gtggcgctgg cctccagccc gacccataaa tccgatcagg cggttgcggg
    10861 ggcggtagtc atcgcatggg cgatttctcg tgccattgac ggagaaagct ggtcagcgat
    10921 tgttgattca ctgccttcaa ttgcgcgaca tgctcaacaa aaacgcatca ctaccttcag
    10981 cgcctcactg gcagcacgtc tggagattgc gctgaaaatt gtgcgcaatg ctgacggcac
    11041 cgaatccgcc agcgaacagc tttaccaggt cgttggcgca ggtaccagta ctattgagtc
    11101 cgttccgtgc gccattgcgc tggttgaact ggcacaaacc gacccgaacc gttgcgccgt
    11161 cctgtgcgct aaccttggcg gcgacacaga caccatcggt gctatggcga cggcaatttg
    11221 cggcgcgttg catggcgtta acgctatcga tcctgcgtta aaggcggaac tggatgcagt
    11281 aaatcagctt gatttcaacc gctatgccac ggcgctggcg aagtatcgcc aacaacggga
    11341 ggcgatatga gcggcgctcg attacacacg ctgctgcctg aattagccac gcgtcagccg
    11401 gtgatggtcg tcggcgcggc ggtcattgat gtgatcgccg acgcttatgc cctcccctgg
    11461 cgtgggtgcg atatcgaact gaaacagcag agcgttaacg ttggcggctg cgccctgaat
    11521 attgccgtgg cattaaagcg cctcggcatc gaagcgggta atgccttgcc gctcggtcag
    11581 ggggtgtggg cggagattat tcgcaaccgc atggcaaaag aggacttaat cagcctgatc
    11641 gataacgccg aaggtgataa cggctggtgt ctggcgctgg ttgagccgga tggcgaacgc
    11701 acttttatgt cattcagcgg tgtggaaaat caatggaatc gccagtggct ggcgcgatta
    11761 accgttgcgc ctggcagcct gctctatttt tccggttatc aactggcctc gccctgcggc
    11821 gaattgttag tggaatggct ggaaaagcta caagacgtga cgccgtttat cgattttggc
    11881 ccacgtattg gcgatatccc ggatgcatta ctggcgcgga tcatggcctg tcgaccttta
    11941 gtgtcactca atcgtcaaga ggctgagatt gccgccgaac gttttgcttt atccgcagag
    12001 ataacaacac ttggcaagca atggcaggag aaatttgccg cgccgttgat cgttcgcctc
    12061 gataaagaag gcgcatggta tttcagcaac gacgcttctg gctgcattcc ggcatttcca
    12121 acgcaagttg tagacaccat tggggcgggc gacagtcatg ccggcggcgt acttgccggg
    12181 ctggcctctg gtctgccact ggcggatgcc gtattactgg gcaatgcagt ggcgtcgtgg
    12241 gttgtcgggc atcggggcgg tgattgtgcg ccaacgcgcg aggaactact cctcgcacac
    12301 aaaaacgtat agatcgctgc gacagtggct aatgctgtac tcaataggcc gttgctgttg
    12361 gtcaagcgcc acttgcttga tcaccagcac cggtattttg ctatctaact gaatatgtga
    12421 ctgaaactcg gcatccggca tccgggcgct aacgcgggaa cgcgtacgtt gtgggtaaat
    12481 gtgctggctg cggaaatagt cataaagaga aatcccgatg gcatcaacat catgaattaa
    12541 atgcgccgga acccacgatt cctcaatcga taccgcctct tcatcgacat agcgaatacg
    12601 cttaagcaaa aagacatcac ttcccgcttc gaccgccagc tgctgcgcga cttcttcggg
    12661 acatttcacg acacgcttgt taacccatag cgtatcgggc tttttcccgc gcaataccac
    12721 ctgctgagaa aacccccgcg cttctttcag cgagtattcg aagatgttgt tgatctgcgt
    12781 gccgtaacca cgcgaacgcg tcaccacgcc ctcttcttcc agcgcctgca tcgccttgcg
    12841 caccgtaatg cgcgaaacgc cggttaactg acttaaatca cgctcaccgg gcaaaatatt
    12901 gccatgctcc agcaccccgc tgcgcacggc attttttacc gtttcggcaa acttaatata
    12961 aagcggcgtg ttatccgccg ctaaaatacg ttcgttcagt tgggcaatca gctgggtatg
    13021 cgcttgttcc atttgtcttt ttcctgccgg ggaatctggt gggagtatat cactgcttct
    13081 tatttaaggc gtttctttaa tcccatcaac gaatgcctgc aactcctcca ccgtgccatt
    13141 aaacacatta aaatccaccg gaccattaat gccatcgacc tgtccacggt cggaatgctg
    13201 ccagaagcgc cacgccatgc cgtcattgtc cgggcgacgt tgataatagt gagccaccca
    13261 ccacggatat tcagtgaaat agcccgccag attggcgtga taaaaaacgg ctcctgagta
    13321 aataattggc ttttttcccg tacgtttttc gaccattttt agccactgac ttacccgctt
    13381 gcgtaattct tttgccgata attttccccg ttcttctacg tccagcacgg cagggaaatc
    13441 gccttgtgag aaatccaccg tttgcagaaa taatctcgcc tgaactgaag cagataccga
    13501 cggggaaaaa taatgatacg ccccgcgcag caggccattt tcgcggctta gttgccagtt
    13561 acgcgaaaaa taggggtcca ccagcttttc gccttccgtc gccttaataa aggcaaattg
    13621 taagcgaata ccgttatcgc gcatttttgc cacccgctgc cagtcgatcc gctcctgcca
    13681 gcgcgaaaca tctattccgt gaatggtgta actggcagga atgcgaatag cgaaagattt
    13741 aaccgggcga taaccgtaga agtgaatgta gtctttaatc tgaattgctg tcgaatagaa
    13801 aaagttcacc gtctgacgcg gataaattgc aacgatggaa attagcccaa gagcgcataa
    13861 taaaacggtg aatttttttc gactggtgat tctcaactgc attggtttca tccctgaatg
    13921 tcagcaccaa aggctgacaa taaccaaagc agctatagta cggtgctcac caccaggcgt
    13981 ggaagtggtg aaccggacca ataccgtgac caacttccag cgtgtcggcc tgggctaatg
    14041 ccgatgaaag ccagcttttt gcctcctgta cggtgtcagc ccagtttgta tggcgcgggc
    14101 gtagtgcagc caacgccgca gagagcgtac aaccagtgcc gtgagtgttt ttggtcataa
    14161 tgcgctgtgc ggtaaaccgt tgttcaccct cgcgggtaaa cagccagtcc gggctttgct
    14221 catcatccag atgaccacct ttcattagca ctgcgccaca gcccatcgcc aacagcgatc
    14281 gcccttgttc cagcatttcc tgttcggtgc gcgcgtgtgg cgcgtcgagc aaggcggctg
    14341 cttcgggcaa gtttggtgtt attaatgaaa cctgtggcaa taatcgactg cgtagcgtgg
    14401 caactgccga aggtgaaagc agcgggtcgc cgctttttgc cagcataacg gtgtcgagta
    14461 ccacgttttg gatctgataa cgttgcaacc gttctgccac cgcttcaaca atatcggttt
    14521 ccgccaacat accgatttta gtggtgtcga ttcgcacatc gctgaacacc gaatcgagct
    14581 gggcggcgac aaaatcaggc tcaatgcgat acaccgactg tacgccacgg gtattttgcg
    14641 ccactagtgc agtaataact gagcaaccat aagcgccaag tgccgagaag gttttaagat
    14701 cggcctgaat ccccgcacca ccactcggat cagtaccggc aatcgtcaga gcgttaattc
    14761 gtttcatgcc tgcacctcct gcgccaattg ccagagcgca tcaaggaaat gtggaacaaa
    14821 actacctggc ccctcgcttc tggcgactgc gcgttctccg gcttgtttca tccagtgaca
    14881 ggcagatgcg acattttcca gcgtatcgcc tggtagcgca caacaggcag cgacaaccgc
    14941 cgataatgca cagccagttc ctaccacttt ggtcattagc ggatcgccac cgtgaatgcc
    15001 aacgatacga tgtccatcgg taacataatc cacctcgcca gtgaccacga cgattgcgcc
    15061 agtttcccgt gccagtgttt gtgcagcggg tatcgcgtta gctgcggcat cagtggtatc
    15121 cactccccgt ccaccattag caacgccagc taatgccatg atttccgaag cattaccacg
    15181 tatggcggtt ggcttatggg acagaagttc cacacaaaaa cggcggcgat aatcgagcgc
    15241 accaaccgca accggatcaa gcgtccaggg cgtttgtgat cgtgttgctt gctcaacggc
    15301 agcactcatc gcttgcgctc gtggctgcgt cagtgtgcca acgttaatca gcaacgcact
    15361 ggcgatagcc gcaaactgac tggcttcttc ggtttcgata accatcgctg gcgatgcacc
    15421 gagcgccagc aaggtattgg cagtaaaggt ttgcaccaca tcgttggtca tacagtgcac
    15481 aagaggggaa tgttggtgaa aaaggtgtaa cgcgtgcgca gattgcgctg aactcagcag
    15541 gtcgacttgc atagtttgct cctgccataa cgtgaagaag caatgacctg gtggtccgtg
    15601 acttccctac gctggcatta tccagatcag gtgatacggg tatttctcag ccttcacgca
    15661 gaagggcacc ccgagtcgtt tggttgcgat gataaatcgc agaggaggat ggtaatgtcc
    15721 agcgcacgcg ttgtaaacgg ggaaaaacgg cagttttaca atcgcgttaa taaattattt
    15781 gatataagaa tccagcacct tcagaacgac atccagatct tcttctcgtt ttagctcatc
    15841 cccctggtga acgatgtgtt ccgtcagatg acctttaatc acttcccgca tcagaccgtt
    15901 taccgcgcca cggatagcag caatctgttg taaaactgca gcgcattcat gcggttcgtc
    15961 gagcattttc ttgagcgcca cgacctggcc ctgaatctta ctggcacgcg ctttcagttt
    16021 ctgtttatca cggattgtat gagacatggc aacacctggt taacaagaat atgaaaaatc
    16081 atagcactat taatctactg gggggtagta tcaggtactg ggggggagta gaatcagatt
    16141 gccgaattaa tactaagaat tattatcatg accgaattta ccactcttct tcagcaagga
    16201 aatgcctggt tcttcatccc cagcgccatc ttacttggtg cgcttcatgg cctggaacca
    16261 gggcactcaa aaacgatgat ggcggcgttt atcatcgcca tcaaaggcac cattaaacag
    16321 gcggtgatgc tcggactggc agcaactatt tcgcataccg cagtggtctg gttaattgcc
    16381 tttggcggga tggtgatcag caaacgcttt actgctcaat cagcagaacc gtggctccag
    16441 ttgatttccg cagtgatcat tattagcacc gcgttctgga tgttctggcg tacctggctc
    16501 ggcgaacgca actggctgga gaatatgcac gagcatgatc atgagcatga gcatcatcac
    16561 cacgatcacg aagatcacca cgaccatgga catcatcacc atcacgaaca tggcgagtat
    16621 caggatgccc atgcacgagc ccatgccaat gacattaaac gacgctttga tggtagagag
    16681 gtcaccaact ggcaaatttt gctatttggc ttaaccggtg gccttatccc ctgcccggca
    16741 gcaattaccg tgctgttgat ttgcattcag ttgaaagccc tgacactggg cgcaacactg
    16801 gtcgtcagtt tcagcactgg cctggcgtta acgcttgtca ccgtaggcgt tggcgcagca
    16861 atcagcgttc agcaggtcgc aaaacgctgg agtggattta acactctcgc taaacgcgct
    16921 ccctattttt ccagtctgtt gattggctta gttggtgtgt atatgggcgt acatggcttc
    16981 atgggcataa tgcgataact caatccttta ggcttgcatg ttatcttcag cccaggatga
    17041 ttcttaaatc agctatttcc gctgactctc tgctcgctaa ggatgctttt agggcatcct
    17101 ttcatttaca ctttttacga aatcatggga tcactaacaa aatatcgctt gtcagttata
    17161 ttgtatggca ggaaagatat gcgactgata ttacagatcc ccaaagtgga gagtttatga
    17221 ccattaaaaa taagatgttg ctgggtgcgc ttttgctggt taccagtgcc gcctgggccg
    17281 caccagccac cgcgggttcg accaatacct cgggaatttc taagtatgag ttaagcagtt
    17341 tcattgctga ctttaagcat ttcaaaccag gtgacaccgt cccagagatg taccgcaccg
    17401 atgagtacaa cattaagcag tggcagttac gtaacctgcc cgcgcctgat gccgggacgc
    17461 actggaccta tatgggtggc gcgtacgtgt tgatcagcga caccgacggt aaaatcatta
    17521 aagcctacga cggtgagatt ttttatcatc gctaaaaaaa agccccctcg gagagagggg
    17581 ggaatgcaga caccttatta ttttttatta ctagccactt gctcgtcttg cttattatta
    17641 gtcgtatttc acattgatta atgcggttgc ctccagtgcg ccagatttaa ctttgtttgt
    17701 atcgtagacg tagtaactgg ccgttatcgg aattgaaacc gttgtatcta taagttttga
    17761 ctttgccgca atttctttta aatttgtata tcctttactg ctgttagtga tatttaaaag
    17821 tgtgactggc gtaccattac tttcaagaat aaatcctacg ccattatctt ccgcaccttt
    17881 caggatcgtg ttgctgttgg ttaacaagtc agaacttgat aaatacacct gcattttccg
    17941 tgatgtttga ttttgttgac ctaataaatt tttacaacgc agaataatat cccctttttt
    18001 actatttatt gttgttttgt tacctgtggc tggtaattgt gaaacgggaa tactctctaa
    18061 cgtaattaat aaatcgtccg gtttgcaggt tgtggttatc gggttgtatg acaatgtcag
    18121 attaaaggaa tagacacctg cattttgtgc caatgcattt ctgtagctat ttacacatgc
    18181 atcccatgac ttaccaccta ataaacacag tagaaattgt actgcggcac tcccgcgtgt
    18241 cgatgatgaa gcactggata atgtcgcaac tccattaatc atcactgaac caccgccccc
    18301 cgcagaaaca ttcacttttg cattactggc cgcaggtgtc accgttaccg aataaccact
    18361 ggtgaaactg gtctgattat ttgacaacgt aatgttgttt ttattggtcc attcaaaatg
    18421 taaatttaat ttaatggtat cgtcataaag tccaacaata atatccgacg ggctcatagg
    18481 gatgatagta tttgtactca cgcatgacga atcattaaaa ttatgctcat atgtcgctat
    18541 ttgtgtttga ctaagtgcaa aatccccctg aacaaccaaa ttgtgctgaa cagataactc
    18601 actataacat gccgcatagc tcataggcat aaccatcatc attactataa atagcattat
    18661 gcgaatttcc atagaaccac ctgactatta ctggcaaata taatttctac tttcatcaat
    18721 ctctttaccg aaggtgattg tgcatgaaag cccttgttgc ttatcaattg ccacattaac
    18781 cgatggcggt acttcattgg tgcgaataaa taactggctt ccctggccga caacaccaat
    18841 attatgacca tggatatcat tgacttcata accaaacgtt aatggttgcc catccgctct
    18901 taatgctttt ataaaccatg gctttcgctg atcggtatca aaattaacca gtacaaccgc
    18961 gccgcgataa ggggcggcaa ttttccggtt gccacgtaat tctgcttcgc tatcgctttg
    19021 tgacacatcc aacatcaggt aattttcccg ataaggtgtc attccgtcgt ataccaccac
    19081 tccattacgg tttgttgtgc gatatttttg cccattgaca taagcatctt taattcctgg
    19141 cgcattcatc acagcaaacg tttcagaaag acggtttgcc agattaacgc cacccgacca
    19201 ggcgacaatg ccccctgaaa cactggctcc agcctgtcga taagcactcg actgactata
    19261 actgccattc actgtcgcaa ccggcgcgtt ccaggttaaa ttcgccccag ctgtcgtttc
    19321 atttccctga tactgataac tcaggttgac cccatagtta aactgatccc ggcttccaac
    19381 ggttcctgat aatcccgtat tatttgaggc aaccccctga tcatcaaacg tcgttgagtt
    19441 agacatatat atttgccgac gaggcgtcgt aacgtcatca ccccaatcaa agggaatcga
    19501 tataaaaata ttaaaacgtt tctcttcatg atgattctcg tcataagcat ggcttgccgc
    19561 gagggtatag cttatccttc gcaagttgtt ggaataactc aactgataat ccttactgct
    19621 gccgctacgc ccccagtaat ctcgccataa cgtacttaag gacacagaac cccagccttc
    19681 tggcaatgac tgactcatat tggcagagaa gctattttta cggccaaaat cgttctggta
    19741 ataatcggca atgtcataga tatcgttttc atcatggcga taattatctt tattgtttgc
    19801 ccatacgtga tcattaaatg tccgataatc acgcgacgaa taacgccagg ccgccagacc
    19861 aaaacgtgtc gacgtttggc tcacaaattt gttgtaggca atttgataac tttgcccgtc
    19921 aaacacatcg ccgttgtctt gtttactatg cgacttcgtg gcatcgacgg aaatggcacc
    19981 aatgcgtgta ttccagccag tccccaaagt gaacgcgtaa taattattcg cgaccatcga
    20041 gccaccatac agcgttaata agttattaaa accatactga tgacccgcct ggacaaaatc
    20101 actttgtttg ctcgcccctt caatatggct acgacccgcc gcaaaatcat atttcgacac
    20161 gccgggttgc agcatatttg gtaccgctgc ataaggcacc agataggtgg ttaccgaacc
    20221 gtccgcctct ttcacgctga catcaagatc cgctccacca ccggccaact gcaaatctgt
    20281 aatcgcaaac gggccaggag gaacctcttt ctgataaacc acaaaaccat tctgttcaat
    20341 agttaccagc gcgttactct gagcaatccc ctgcacccgt ggcgtaaaat tttgtttcga
    20401 gttaggcaac atctgcatat cacgaaacaa ccgcacaccg ctgaagcgaa cagaatcaaa
    20461 aatatcgctt gatgtgtaca tatcacccac acgaagcgtg ccgagaagtt gggcaaatcc
    20521 acgttccaga tacagggtat tgcttttcca cacccctgga ttgttatttg ttttactgaa
    20581 actggcatca gaatgcagtt gccactcctg taaatttaac ccgctgttaa aacgtacata
    20641 tgtactcttg ctattaccgg acgctttata atcgctgtaa tactgactca cataataaga
    20701 ggtataaaac gcattaatac cccgctccca gttttctggt ggaacatagc cactttccag
    20761 ctcttccacc caggcctgcg ggacactgaa atcgagacga aaaactccga tatcccaggt
    20821 atagctcccg ccctgaataa gttgcttaaa tgttaaacat tgcttaccgc tggcaaagtt
    20881 atcggtatta atgcctaacc gcttgatcat ttcccttgat aaacatgttt cttgcgggtt
    20941 gtctttaaca acgatctcat atttcccacg ccattgctta ttgacataaa tatcgatgtc
    21001 atactgcccc ggcaagggct gattatcttc aagacgaata tttgaaacct gctggtcttt
    21061 cattccacct atcatgaaat gggtatcaaa ggtttcttca gctgcataag cttcaatgcc
    21121 gatcaataac gctacgattg ctgatgcaag tggggtcatt ctcaacatag gcagctcctg
    21181 caattaaatt ttgtcactaa tataattgcc atggtcatcg ataatggtca gatgccagtt
    21241 atttgcatta ttacttttga cattaacact ctgactttct aagggggcaa tcataatagt
    21301 ttcataattg actttgacat tattagcttt gacatccgaa atcgtcaccc aattagctga
    21361 gtcatttttt atcaccaatc cattgccact gcgatttacc agtaactttt taaaggtcac
    21421 tttatttacc ggagcaatac ccgctggccg gtaaaacaac ttaattctgt tttgcatcgc
    21481 aaacttcagt gcattcttac cttcttgttc tggact
//
LOCUS       DADSYM010000077        21194 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2415
            SAMN08039579-rid11723173.denovo.077, whole genome shotgun sequence.
ACCESSION   DADSYM010000077 DADSYM010000000
VERSION     DADSYM010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039579
            Sequence Read Archive: SRR6362533
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 21194)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21194)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980162.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 03:39:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,484
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 4,347
            CDSs (with protein)               :: 4,347
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 43 of 85
            Pseudo Genes (incomplete)         :: 37 of 85
            Pseudo Genes (internal stop)      :: 19 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21194
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039579-rid11723173.denovo.077"
                     /strain="LGE2415"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8302 N 95.9628 W"
                     /collection_date="2015-08-24"
     gene            complement(81..1121)
                     /locus_tag="J1Y83_002516"
     CDS             complement(81..1121)
                     /locus_tag="J1Y83_002516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001521390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HBA7630658.1"
                     /translation="MTGQSSSQAATPIQWWKPALFFLVVIAGLWYVKWQPYYGKAFTA
                     AETHSIGKSILAQADANPWQAALDYAMIYFLAVWKAAVLGVILGSLIQVLIPRDWLLR
                     TLGQSRFRGTLLGTLFSLPGMMCTCCAAPVAAGMRRQQVSMGGALAFWMGNPLLNPAT
                     LVFMGFVLGWGFAAIRLVAGLVMVLLIATLVQKWVRETPQTQAPVEIDIPESQGGFFS
                     RWGRALWTLFWSTIPVYILAVLVLGAARVWLFPHADGAVDNSLMWVVAMAVAGCLFVI
                     PTAAEIPIVQTMMLAGMGTAPALALLMTLPAVSLPSLIMLRKAFPTKALWLTGAMVAV
                     SGVIVGGLALLV"
     gene            complement(1194..1829)
                     /locus_tag="J1Y83_002517"
     CDS             complement(1194..1829)
                     /locus_tag="J1Y83_002517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312060.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HBA7630659.1"
                     /translation="MSQVLITGATGLVGGHLLRMLINEPKVNAIAAPTRRPLGDMPGV
                     FNPHDPQLTDALAQVTDPIDIVFCCLGTTRREAGSKEAFIHADYTLVVDTALTGRRLG
                     AQHMLVVSAMGANAHSPFFYNRVKGEMEEALIAQNWPKLTIARPSMLLGDRSKQRMNE
                     TLFAPLFRLLPGNWKSIDARDVARVMLAEAMRPEHEGVTILSSSELRKRAE"
     gene            1957..2475
                     /gene="yhbO"
                     /locus_tag="J1Y83_002518"
     CDS             1957..2475
                     /gene="yhbO"
                     /locus_tag="J1Y83_002518"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417622.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein/nucleic acid deglycase"
                     /protein_id="HBA7630660.1"
                     /translation="MSKKIAVLITDEFEDSEFTSPADEFRKAGHEVITIEKQAGKTVK
                     GKKGEASVAIDKSIDEVTPAEFDALLLPGGHSPDYLRGDNRFVTFTRDFVNSGKPVFA
                     ICHGPQLLISADVIRGRKLTAVKPIVIDVKNAGAEFYDQEVVVDKDQLVTSRTPDDLP
                     AFNREALRLLGA"
     gene            complement(2455..2898)
                     /locus_tag="J1Y83_002519"
     CDS             complement(2455..2898)
                     /locus_tag="J1Y83_002519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhbP family protein"
                     /protein_id="HBA7630661.1"
                     /translation="METLTAISRWLAKQHVVTWCVQQEGELWCANAFYLFDAQKVAFY
                     ILTEEKTRHAQMSGPQAAIAGTVNGQPKTVALIRGVQFKGEIRRLEGEESDLARKAYN
                     RRFPVARMLSAPVWEIRLDEIKFTDNTLGFGKKMIWLRNSGTEQA"
     gene            2949..3251
                     /gene="yhbQ"
                     /locus_tag="J1Y83_002520"
     CDS             2949..3251
                     /gene="yhbQ"
                     /locus_tag="J1Y83_002520"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708956.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage response exodeoxyribonuclease YhbQ"
                     /protein_id="HBA7630662.1"
                     /translation="MTPWFLYLIRTADNKLYTGITTDVERRYQQHQSGKGAKALRGKG
                     ELTLAFSAPVGNRSLALRAEYRVKQLTKRQKERLVAEGAVFAELLNSLQTPEIKSD"
     gene            complement(3238..3741)
                     /locus_tag="J1Y83_002521"
     CDS             complement(3238..3741)
                     /locus_tag="J1Y83_002521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HBA7630663.1"
                     /translation="MLIRVEIPIDAPGIDALLRRSFESDAEAKLVHDLREDGFLTLGL
                     VATDDEGQVIGYVAFSPVDVQGEDLQWVGMAPLAVDEKYRGQGLARQLVYEGLDSLNE
                     FGYAAVVTLGDPALYSRFGFELAAHHDLRCRWPGTESAFQVHRLADDALNGVTGLVEY
                     HEHFNRF"
     gene            complement(3735..4259)
                     /locus_tag="J1Y83_002522"
     CDS             complement(3735..4259)
                     /locus_tag="J1Y83_002522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 domain-containing protein"
                     /protein_id="HBA7630664.1"
                     /translation="MLDKLRSRIVHLGPSLLSVPVKLTPFALKRQVLEQVLSWQFRQA
                     LDDGELEFLEGRWLSIHVRDIDLQWFTSVVNGKLVVSQNAQADVSFSADASDLLMIAA
                     RKQDPDTLFFQRRLVIEGDTELGLYVKNLMDAIELEQMPKALRMMLLQLADFVEAGMK
                     TAPETKQTSVGEPC"
     gene            4468..5463
                     /locus_tag="J1Y83_002523"
     CDS             4468..5463
                     /locus_tag="J1Y83_002523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017457364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HBA7630665.1"
                     /translation="MELLCPAGNLPALKAAIENGADAVYIGLKDDTNARHFAGLNFTE
                     KKLQEAVSFVHQHRRKLHIAINTFTHPDGYARWQRAVDMAAQLGADALILADLAMLEY
                     TAERYPHIERHVSVQASATNEEAINFYHRHFDVARVVLPRVLSIHQVKQLARVTPVPL
                     EVFAFGSLCIMSEGRCYLSSYLTGESPNTVGACSPARFVRWQQTPQGLESRLNEVLID
                     RYQDGENAGYPTLCKGRYLVDGERYHALEEPTSLNTLELLPELMAANIASVKIEGRQR
                     SPAYVSQVAKVWRQAIDRCKADPQNFVPQSAWMETLGSMSEGTQTTLGAYHRKWQ"
     gene            5472..6350
                     /locus_tag="J1Y83_002524"
     CDS             5472..6350
                     /locus_tag="J1Y83_002524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HBA7630666.1"
                     /translation="MKYSLGPVLWYWPKETLEDFYQQAAASSADVIYLGEAVCSKRRA
                     TKVGDWLEMAKSLAGSGKQIVLSTLALVQASSELGELKRYVENGEFLIEASDLGVVNM
                     CAERKLPFVAGHALNCYNAVTLKILLKQGMMRWCMPVELSRDWLVNLLNQCDELGIRN
                     QFEVEVLSYGHLPLAYSARCFTARSEDRPKDECETCCIKYPNGRNVLSQENQQVFVLN
                     GIQTMSGYVYNLGNELASMQGLVDVVRLSPQGTDTFAMLDAFRANENGAAPLPLTANS
                     DCNGYWRRLAGLELQA"
     gene            6431..7438
                     /locus_tag="J1Y83_002525"
     CDS             6431..7438
                     /locus_tag="J1Y83_002525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="luciferase-like monooxygenase"
                     /protein_id="HBA7630667.1"
                     /translation="MTDKTIAFSLLDLAPIPEGSSAREAFSHSLDLARLAEKRGYHRY
                     WLAEHHNMTGIASAATSVLIGYLAANTTTLHLGSGGVMLPNHSPLVIAEQFGTLNTLY
                     PGRIDLGLGRAPGSDQRTMMALRRHMSGDIDNFPRDVAELVDWFDARDPNPHVRPVPG
                     YGEKIPVWLLGSSLYSAQLAAQLGLPFAFASHFAPDMLFQALHLYRSNFKPSARLEKP
                     YAMVCINIIAADSNRDAEFLFTSMQQAFVKLRRGETGQLPPPIQNMDQFWSPSEQYGV
                     QQALSMSLVGDKAKVRHGLQSILRETDADEIMVNGQIFDHQARLHSFELAMDVKEELL
                     G"
     gene            complement(7556..8800)
                     /gene="mtr"
                     /locus_tag="J1Y83_002526"
     CDS             complement(7556..8800)
                     /gene="mtr"
                     /locus_tag="J1Y83_002526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005131807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan permease"
                     /protein_id="HBA7630668.1"
                     /translation="MATLTTTQTSPSLLGGVVIIGGTIIGAGMFSLPVVMSGAWFFWS
                     MAALIFTWFCMLHSGLMILEANLNYRIGSSFDTITKDLLGKGWNVVNGISIAFVLYIL
                     TYAYISASGSILHHTFAEMSLNVPARAAGFGFALLVAFVVWLSTKAVSRMTAIVLGAK
                     VITFFLTFGSLLGHVQPTTLFNVAESNASYAPYLLMTLPFCLASFGYHGNVPSLMKYY
                     GKDPKTIVKCLVYGTLMALALYTIWLLATMGNIPRPEFIGIAEKGGNIDVLVQALSGV
                     LNSRSLDLLLVVFSNFAVASSFLGVTLGLFDYLADLFGFDDSAMGRLKTALLTFAPPV
                     VGGLLFPNGFLYAIGYAGLAATIWAAIVPALLARASRKRFGSPKFRVWGGKPMIMLIL
                     VFGVGNALVHILSSFNVLPVYQ"
     gene            complement(8954..10843)
                     /locus_tag="J1Y83_002527"
     CDS             complement(8954..10843)
                     /locus_tag="J1Y83_002527"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417631.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH family ATP-dependent RNA helicase"
                     /protein_id="HBA7630669.1"
                     /translation="MAEFETTFADLGLKAPILEALNDLGYEKPSPIQAECIPHLLNGR
                     DVLGMAQTGSGKTAAFSLPLLQNLDPELKAPQILVLAPTRELAVQVAEAMTDFSKHMR
                     GVNVVALYGGQRYDVQLRALRQGPQIVVGTPGRLLDHLKRGTLDLSKLSGLVLDEADE
                     MLRMGFIEDVETIMAQIPEGHQTALFSATMPEAIRRITRRFMKEPQEVRIQSSVTTRP
                     DISQSYWTVWGMRKNEALVRFLEAEDFDAAIIFVRTKNATLEVAEALERNGYNSAALN
                     GDMNQALREQTLERLKDGRLDILIATDVAARGLDVERISLVVNYDIPMDSESYVHRIG
                     RTGRAGRAGRALLFVENRERRLLRNIERTMKLTIPEVELPNAELLGKRRLEKFAAKVQ
                     QQLESSDLDQYRALLSKIQPTAEGEELDLETLAAALLKMAQGERTLIVPPDAPMRPKR
                     EFRDRDDRGPRDRNDRGPRGDREDRPRRERRDVGDMQLYRIEVGRDDGVEVRHIVGAI
                     ANEGDISSRYIGNIKLFASHSTIELPKGMPGEVLQHFTRTRILNKPMNMQLLGDAQPH
                     TGGERRGGGRGFGGERREGGRNFSGERREGGRGDGRRFSGERREGRAPRRDDSTGRRR
                     FGGDA"
     gene            complement(10836..10916)
                     /gene="yrbN"
                     /locus_tag="J1Y83_002528"
     CDS             complement(10836..10916)
                     /gene="yrbN"
                     /locus_tag="J1Y83_002528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YrbN"
                     /protein_id="HBA7630670.1"
                     /translation="MKIADQFHDELCRLAAINFEAHVLHG"
     gene            complement(11023..11907)
                     /gene="nlpI"
                     /locus_tag="J1Y83_002529"
     CDS             complement(11023..11907)
                     /gene="nlpI"
                     /locus_tag="J1Y83_002529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020802045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein NlpI"
                     /protein_id="HBA7630671.1"
                     /translation="MKPFLRWCFVATALTLAGCSNTSWRKSEVLAVPLQPTLQQEVIL
                     ARMEQILASRALTDDERAQLLYERGVLYDSLGLRALARNDFSQALAIRPDMPEVFNYL
                     GIYLTQAGNFDAAYEAFDSVLELDPTYNYAHLNRGIALYYGGRDKLAQDDLLAFYQDD
                     PNDPFRSLWLYLAEQKLDEKQAKEVLKQHFEKSDKEQWGWNIVEFYLGNISEQTLMER
                     LKADATDNTSLAEHLSETNFYLGKYYLSLGDLDSATALFKLAVANNVHNFVEHRYALL
                     ELSLLGQDQDDLAESDQQ"
     gene            complement(12016..14151)
                     /gene="pnp"
                     /locus_tag="J1Y83_002530"
     CDS             complement(12016..14151)
                     /gene="pnp"
                     /locus_tag="J1Y83_002530"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HBA7630672.1"
                     /translation="MLNPIVRKFQYGQHTVTLETGMMARQATAAVMVSMDDTAVFVTV
                     VGQKKAKPGQDFFPLTVNYQERTYAAGRIPGSFFRREGRPSEGETLIARLIDRPIRPL
                     FPEGFVNEVQVIATVVSVNPQVNPDIVAMIGASAALSLSGIPFNGPIGAARVGYINDQ
                     YVLNPTQDELKESKLDLVVAGTEAAVLMVESEAELLSEDQMLGAVVFGHEQQQVVIQN
                     INELVKEAGKPRWDWQPEPVNEALNARVAALAEARLSDAYRITDKQERYAQVDVIKSE
                     TIATLLAEDETLDENELGEILHAIEKNVVRSRVLAGEPRIDGREKDMIRGLDVRTGVL
                     PRTHGSALFTRGETQALVTATLGTARDAQVLDELMGERTDTFLFHYNFPPYSVGETGM
                     VGSPKRREIGHGRLAKRGVLAVMPDMDKFPYTVRVVSEITESNGSSSMASVCGASLAL
                     MDAGVPIKAAVAGIAMGLVKEGDNYVVLSDILGDEDHLGDMDFKVAGSRDGISALQMD
                     IKIEGITKEIMQVALNQAKGARLHILGVMEQAINAPRGDISEFAPRIHTIKINPDKIK
                     DVIGKGGSVIRALTEETGTTIEIEDDGTVKIAATDGEKAKHAIRRIEEITAEIEVGRV
                     YNGKVTRIVDFGAFVAIGGGKEGLVHISQIADKRVEKVTDYLQMGQEVPVKVLEVDRQ
                     GRIRLSIKEATEQSQPAAAPEAPAAEQGE"
     gene            complement(14398..14667)
                     /gene="rpsO"
                     /locus_tag="J1Y83_002531"
     CDS             complement(14398..14667)
                     /gene="rpsO"
                     /locus_tag="J1Y83_002531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004933502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HBA7630673.1"
                     /translation="MSLSTEATAKIVSEFGRDANDTGSTEVQVALLTAQINHLQGHFA
                     EHKKDHHSRRGLLRMVSQRRKLLDYLKRKDVARYTQLIERLGLRR"
     gene            complement(14816..15760)
                     /gene="truB"
                     /locus_tag="J1Y83_002532"
     CDS             complement(14816..15760)
                     /gene="truB"
                     /locus_tag="J1Y83_002532"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HBA7630674.1"
                     /translation="MSRPRRRGRDINGVLLLDKPQGMSSNDALQKVKRIYNANRAGHT
                     GALDPLATGMLPICLGEATKFSQYLLDSDKRYRVIARLGQRTDTSDADGQIVEERPVT
                     FSAEQLAAALDTFRGDIEQIPSMYSALKYQGKKLYEYARQGIEVPREARPITVYELLF
                     IRHEGNELELEIHCSKGTYIRTIIDDLGEKLGCGAHVIYLRRLAVSKYPVERMVTLEH
                     LRELVEQAEQQDIPAAELLDPLLMPMDSPASDYPVVNLPLTSSVYFKNGNPVRTSGAP
                     LEGLVRVTEGENGKFIGMGEIDDEGRVAPRRLVVEYPA"
     gene            complement(15760..16161)
                     /gene="rbfA"
                     /locus_tag="J1Y83_002533"
     CDS             complement(15760..16161)
                     /gene="rbfA"
                     /locus_tag="J1Y83_002533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001712908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HBA7630675.1"
                     /translation="MAKEFGRPQRVAQEMQKEIALILQREIKDPRLGMMTTVSGVEMS
                     RDLAYAKVYVTFLNDKDEDAVKAGIKALQEASGFIRSLLGKAMRLRIVPELTFFYDNS
                     LVEGMRMSNLVTSVVKHDEERRVNPDDSKED"
     gene            complement(16325..18997)
                     /gene="infB"
                     /locus_tag="J1Y83_002534"
     CDS             complement(16325..18997)
                     /gene="infB"
                     /locus_tag="J1Y83_002534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HBA7630676.1"
                     /translation="MTDVTIKTLAAERQTSVERLVQQFADAGIRKSADDSVSAQEKQT
                     LIDHLNQKNSGPDKLTLQRKTRSTLNIPGTGGKSKSVQIEVRKKRTFVKRDPQEAERL
                     AAEEQAQREAEEQARREAEESAKREAQQKAEREAAEQAKREAAEQAKREAAEKDKVSN
                     QQDDMTKNAQAEKARREQEAAELKRKAEEEARRKLEEEARRVAEEARRMAEENKWTDN
                     AEPTEDSSDYHVTTSQHARQAEDESDREVEGGRGRGRNAKAARPKKGNKHAESKADRE
                     EARAAVRGGKGGKRKGSSLQQGFQKPAQAVNRDVVIGETITVGELANKMAVKGSQVIK
                     AMMKLGAMATINQVIDQETAQLVAEEMGHKVILRRENELEEAVMSDRDTGAAAEPRAP
                     VVTIMGHVDHGKTSLLDYIRSTKVASGEAGGITQHIGAYHVETENGMITFLDTPGHAA
                     FTSMRARGAQATDIVVLVVAADDGVMPQTIEAIQHAKAAGVPVVVAVNKIDKPEADPD
                     RVKNELSQYGILPEEWGGESQFVHVSAKAGTGIDELLDAILLQAEVLELKAVRKGMAS
                     GAVIESFLDKGRGPVATVLVREGTLHKGDIVLCGFEYGRVRAMRNELGQEVLEAGPSI
                     PVEILGLSGVPAAGDEVTVVRDEKKAREVALYRQGKFREVKLARQQKSKLENMFANMT
                     EGEVHEVNIVLKADVQGSVEAISDSLLKLSTDEVKVKIIGSGVGGITETDATLAAASN
                     AILVGFNVRADASARKVIEAESLDLRYYSVIYNLIDEVKAAMSGMLSPELKQQIIGLA
                     EVRDVFKSPKFGAIAGCMVTEGVVKRHNPIRVLRDNVVIYEGELESLRRFKDDVNEVR
                     NGMECGIGVKNYNDVRTGDVIEVFEIIEIQRTIA"
     gene            complement(19022..20509)
                     /gene="nusA"
                     /locus_tag="J1Y83_002535"
     CDS             complement(19022..20509)
                     /gene="nusA"
                     /locus_tag="J1Y83_002535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005131833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HBA7630677.1"
                     /translation="MNKEILAVVEAVSNEKALPREKIFEALESALATATKKKYEQEID
                     VRVQIDRKSGDFDTFRRWLVVDEVTQPTKEITLEAARYEDESLNLGDYVEDQIESVTF
                     DRITTQTAKQVIVQKVREAERAMVVDQFREHEGEIITGVVKKVNRDNISLDLGNNAEA
                     VILREDMLPRENFRPGDRVRGVLYSVRPEARGAQLFVTRSKPEMLIELFRIEVPEIGE
                     EVIEIKAAARDPGSRAKIAVKTNDKRIDPVGACVGMRGARVQAVSTELGGERIDIVLW
                     DDNPAQFVINAMAPADVASIVVDEDKHTMDIAVEAGNLAQAIGRNGQNVRLASQLSGW
                     ELNVMTVDDLQAKHQAEAHAAIDTFTKYLDIDEDFATVLVEEGFSTLEELAYVPMKEL
                     LEIEGLDEPTVEALRERAKNALATIAQAQEESLGDNKPADDLLNLEGVDRDLAFKLAA
                     RGVCTLEDLAEQGIDDLADIEGLTDEKAGALIMAARNICWFGDEA"
     gene            complement(20537..20989)
                     /gene="rimP"
                     /locus_tag="J1Y83_002536"
     CDS             complement(20537..20989)
                     /gene="rimP"
                     /locus_tag="J1Y83_002536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004125659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HBA7630678.1"
                     /translation="MSTLEQKLTEMITAPVEALGFELVGIEFIRGRTSTLRIYIDSED
                     GINVDDCADVSHQVSAVLDVEDPITVAYNLEVSSPGLDRPLFTAEHYARFVGEEVTLV
                     LRMAVQNRRKWQGVIKAVDGEMITVTVEGKDEVFALSNIQKANLVPHF"
BASE COUNT         4990 a         5987 c         5241 g         4976 t
ORIGIN      
        1 gcgtcttatc aggcctacaa cgatgcaaac cgtaggtcgg ataaggcatt tacgccgcat
       61 ccgacatgtt ttccctcaaa tcaaaccaac agcgccagcc cgccgacaat cacaccagac
      121 actgccacca tcgcccccgt cagccataag gctttcgtcg ggaacgcttt gcgcagcata
      181 atcagcgacg gcaaactcac cgccgggagc gtcatcaaca atgccagcgc cggagcggtt
      241 cccatacctg ccagcatcat cgtttgtaca atcggaattt ctgctgccgt gggaatgaca
      301 aacaagcatc ccgctaccgc catcgccacc acccacatca ggctgttatc gacagcacca
      361 tcggcatggg ggaataacca gacgcgagcg gcacccaaca ccagtactgc aaggatgtaa
      421 accgggatcg tactccagaa aagcgtccat agcgccctgc cccagcggct aaaaaaaccg
      481 ccttgtgatt ccggtatgtc aatttcgacc ggtgcctgcg tttgcggtgt ttcacgcacc
      541 catttttgca ccagcgtcgc aatcagcaac accatcacca gccccgccac cagacgaatc
      601 gccgcaaaac cccagccgag gacgaacccc ataaacacca gcgtcgccgg gtttaacaac
      661 ggattcccca tccagaatgc cagcgcaccg cccatcgaca cctgttggcg acgcattccc
      721 gccgcgaccg gagccgcaca gcaggtacac atcatacccg gcagcgaaaa cagcgttccc
      781 agcagcgtgc cgcgaaagcg cgattgccca agcgtacgca acaaccagtc acgcgggatc
      841 agcacctgaa tcaacgaacc gaggatcacc cccagcaccg ccgctttcca taccgcgagg
      901 aaatagatca tcgcgtaatc caacgccgcc tgccacggat tagcatccgc ctgcgcaagg
      961 atagatttac cgatactgtg ggtttcggca gcagtaaacg ctttgccgta gtaaggttgc
     1021 catttcacat accagagacc ggcaatgaca acgagaaaga aaagcgcggg tttccaccac
     1081 tgaatgggcg ttgccgcctg agatgaagac tgaccagtca tagcattccc caggaataag
     1141 ttatgataat tgagcgcgtg aatattacgc tcactatcaa ttcttgggaa taattattca
     1201 gctcttttgc gtaattctga agagcttaaa atcgtcacac cttcatgctc aggccgcatc
     1261 gcctctgcca gcatgacccg cgccacgtct ctcgcatcaa tggatttcca gttaccgggt
     1321 aacaaacgga acagcggcgc aaaaagcgtt tcgttcatcc gctgtttgct acgatcgccc
     1381 agtaacatcg acgggcgagc aatggtcaat ttcggccagt tctgggcgat taatgcttct
     1441 tccatctccc ctttgacgcg gttatagaaa aacggcgagt gggcattagc acccatagca
     1501 ctgaccacca gcatatgctg cgcaccgagc cgccgcccgg ttaatgcggt atccactacc
     1561 agcgtgtaat cggcatgaat aaacgcctct ttactccccg cttctcgccg cgtggtgccg
     1621 agacaacaaa acacaatgtc gatgggatcg gtgacctgcg ccagcgcgtc ggtcagttgc
     1681 ggatcatggg gattgaacac tcctggcata tctcccaacg gacgtcgcgt cggcgcggct
     1741 atggcgttaa ctttcggttc gttaatcaac atccgcagca ggtgaccgcc caccagcccc
     1801 gttgcgcctg taatcagtac ctgactcatc ttcgctcctt tacagaattg tctgccttgc
     1861 gctccacggc tcatgcacca ggcttaatag atcatgaggt aattatcccc gattgtggaa
     1921 aattcgcctc atccaatgca acaacgcgga ggaagcatga gtaagaaaat cgccgtttta
     1981 atcactgatg aatttgagga ttcagaattt acttcacccg cagacgagtt ccgtaaagcc
     2041 ggacacgaag tgattaccat tgaaaaacaa gcgggtaaaa cggtgaaagg caaaaaagga
     2101 gaagccagcg tggccatcga taaatccatc gatgaagtga cgcccgcaga gttcgatgcc
     2161 ctgctgctac cgggcggtca ttcaccagat tatctgcgtg gtgacaaccg ttttgtcacc
     2221 ttcacacgcg attttgtgaa cagcggcaaa cctgttttcg ccatctgtca cggtccgcag
     2281 ctgctgatca gcgccgatgt gattcggggg cgcaaactga ctgccgttaa accgatcgtg
     2341 attgatgtta aaaacgcggg cgcggaattt tacgatcagg aagtggtggt cgataaagat
     2401 cagttggtta ccagccggac accggacgat ctgcccgcgt ttaaccgcga ggcgttacgc
     2461 ctgctcggtg cctgagttac gtagccaaat cattttttta ccaaagccca gcgtgttgtc
     2521 ggtgaatttg atttcatcga gccggatttc ccacaccggg gccgacagca ttctggcaac
     2581 cggaaagcga cgattgtacg ccttgcgtgc gaggtcgctt tcctcgcctt ccagtctgcg
     2641 gatctcacct ttaaactgca cgccgcgaat taacgctacc gttttcggct gaccgtttac
     2701 cgttccggca attgccgcct gcggcccgct catctgcgca tgacgcgttt tttcttccgt
     2761 caaaatgtag aaggcgactt tctgcgcatc aaaaagataa aaagcattgg cgcaccacag
     2821 ttccccttcc tgctgcacac accaggtgac aacatgttgt tttgccagcc aacggctgat
     2881 ggcggtgagt gtttccattg ctgttctctt ttatactgtg ggcctgacct taacatgcga
     2941 cgcagacgat gacaccctgg tttctttact tgatccgtac cgccgacaat aagctttata
     3001 ccgggatcac cacggatgtc gaacgccgct atcagcagca ccaaagcggc aaaggggcga
     3061 aagcactgcg cgggaaggga gaactaacgc tggcgttttc cgcaccagtg ggcaatcgtt
     3121 cgctggcgtt acgggcagaa tatcgcgtta agcaactgac caaacggcaa aaagagcgtc
     3181 tggtagcgga aggtgcagtg tttgcagaac tgttgaacag cctgcaaacc ccggagatta
     3241 aaagcgattg aagtgctcgt gatactcaac cagaccagtt acaccgttca gcgcgtcatc
     3301 cgctaaacga tgtacctgga aggcactttc agtgcccggc cagcggcaac gcagatcgtg
     3361 atgtgccgcc agttcaaagc cgaaacggct gtaaagcgcc ggatcgccca gcgtcaccac
     3421 tgcggcatag ccgaactcat taagcgaatc gagtccttca tagaccagtt ggcgtgccag
     3481 cccctgtcca cggtattttt catctaccgc caacggtgcc atgccgaccc attgcaggtc
     3541 ttcgccctgc acatcaaccg gactaaatgc cacatagcca atgacctgac cttcgtcatc
     3601 tgtcgccacc agccccagcg tcagaaagcc atcttcacgc agatcgtgaa ccagcttcgc
     3661 ttccgcatca ctttcgaatg aacgacgcag cagggcatca atacccggcg catcaatggg
     3721 aatttctact cgaattagca tggttcacct accgatgtct gtttggtttc aggcgcggtt
     3781 ttcattcccg cctcaacaaa atccgccagt tgcagcagca tcatgcgtaa ggctttcggc
     3841 atttgctcca gttcaatggc gtccatcagg tttttcacat acagccccag ctccgtatcg
     3901 ccttcaatca ccagccgacg ctggaagaag agcgtatccg gatcttgttt acgcgccgca
     3961 atcatcagca gatcgctggc gtcggcacta aaactcacat cagcttgcgc gttctggcta
     4021 acgaccagtt tgccattcac caccgaggta aaccattgca ggtcaatatc acgcacatga
     4081 atacttaacc agcgaccttc aagaaactcc agctcgccat catccagcgc ctgacggaat
     4141 tgccagctta agacctgctc aagaacctgg cgttttagcg caaatggcgt cagttttacc
     4201 ggtacactca acagagatgg ccccaaatgc acaatacggg aacgcagttt atccaacacg
     4261 agttttactc cctgtttcaa caatcatcct attttgccat atcagaaaaa taacataacg
     4321 gtataaatca acaattccat atgaaattgc tgctaccacc aatacaactt taactgcctt
     4381 aaatcaaaaa ttgtcgcagc aaggttaact aaaatcccag ttcgttaaca tttttgcgtt
     4441 ttgataacgc aaccttcagg aaaaattatg gagctgctct gccctgccgg aaatctcccg
     4501 gcgcttaagg cggccatcga aaacggcgca gatgctgttt atatcgggct aaaagatgat
     4561 actaatgccc gtcacttcgc cggccttaac tttaccgaga aaaaattgca ggaagcggtg
     4621 agttttgtcc atcaacatcg ccgcaaactt cacatcgcga ttaacacttt tacgcatccg
     4681 gacggttacg cccgctggca gcgcgccgtg gatatggcgg cgcagctggg tgccgacgcg
     4741 ctgatcctcg ccgacctcgc catgctggag tacaccgccg agcgttatcc gcatattgag
     4801 cgccacgtat cggtgcaggc ttcggcgacc aatgaagagg cgattaactt ttatcatcgc
     4861 cattttgacg ttgcccgcgt ggtgctgccg cgcgtgttgt cgattcatca ggtgaaacaa
     4921 ctggcacggg tcacacctgt accactggaa gtctttgctt tcggcagcct gtgcattatg
     4981 tcggaaggtc gttgctatct gtcgtcgtat ttgacgggtg agtcgcccaa cactgtgggc
     5041 gcgtgttctc cggcccgttt cgtgcgctgg cagcaaacgc cgcaggggct ggaatcccgc
     5101 ctgaacgaag tgctgatcga ccgttatcag gacggcgaaa acgcaggtta tccgacgcta
     5161 tgtaaagggc gttatctggt ggacggcgag cgctatcacg cgctggaaga accaaccagt
     5221 ctcaatactc tggaactgct gccggagtta atggcggcga atattgcttc ggtgaaaatt
     5281 gaaggccgcc agcgtagccc ggcgtatgtc agccaggtgg cgaaagtctg gcgtcaggct
     5341 atcgaccgtt gtaaggccga tccgcaaaac ttcgtaccgc aaagcgcatg gatggagacg
     5401 ctcgggtcga tgtccgaagg cacgcaaact acccttggcg cgtatcaccg taaatggcag
     5461 tgagaaaagc aatgaaatat tccttagggc cggtgctatg gtactggcca aaagagacgc
     5521 tggaagactt ttatcagcag gccgccgcca gcagcgcaga cgtgatttat cttggtgaag
     5581 cggtctgtag caaacgccgg gcaaccaaag ttggcgactg gctggagatg gcaaaatcgc
     5641 tcgccggaag tggcaagcag attgtgctct ccacgctggc gctggtgcag gcatcctccg
     5701 aactgggcga actgaaacgc tatgttgaga acggtgagtt tctgattgaa gccagcgatc
     5761 tcggcgtggt gaatatgtgc gctgaacgca aactgccgtt cgtcgccggg cacgcgctga
     5821 actgctacaa cgcggtgacg ctgaaaatat tgctcaaaca gggcatgatg cgctggtgta
     5881 tgccggtgga gctttcccgc gactggctgg tgaatctgct taatcagtgc gatgagttgg
     5941 gcattcgcaa ccagtttgaa gtggaagtcc tgagctacgg tcatctacct ctggcctact
     6001 ccgcccgctg ctttaccgcg cgttcggaag accgcccgaa agatgagtgt gaaacctgct
     6061 gcattaaata tccgaacggg cgcaacgtac tgtcgcagga aaaccaacaa gtgtttgtac
     6121 tcaacggcat tcagaccatg agcggctacg tttacaacct cggtaacgag ctggcatcca
     6181 tgcaggggct ggtcgatgtg gtccgcctgt caccgcaggg taccgacact ttcgcgatgc
     6241 tcgacgcctt ccgcgctaat gaaaatggcg ctgcgccact accgttgacg gcaaacagcg
     6301 actgtaacgg ctactggcgg cggctggccg ggctggagct gcaagcgtaa gtatatagct
     6361 cactttgtta acaactttaa ctactcttta atgcagtatt aaagactaat cggtaacaaa
     6421 gtgagctgtt atgactgata aaaccattgc gttttcgcta ctcgatctgg cccccatccc
     6481 cgaaggttct tcagcgcggg aagcgttctc ccactctctc gacctcgccc gtctggctga
     6541 aaagcgcggc tatcatcgct actggctggc agaacaccac aatatgaccg gcattgccag
     6601 tgctgcaacg tcggtgttaa ttggctatct ggcggcgaat accaccacgc tgcatctggg
     6661 gtctggcggc gtgatgttgc ctaaccactc accgttggtg atcgccgaac aattcggcac
     6721 gctcaatacg ctctatccgg ggcgaatcga tttggggctg ggccgcgcgc cgggtagcga
     6781 tcagcgaacc atgatggcgc tgcgtcgtca tatgagcggc gatattgata atttcccccg
     6841 cgatgtcgcg gaactggtgg actggtttga cgcccgcgat cccaatccgc atgtgcgccc
     6901 ggtaccaggc tatggcgaga aaatccccgt gtggttgtta ggatccagcc tttacagcgc
     6961 gcaactggcg gcgcagcttg gtctgccgtt tgcgtttgcc tcacacttcg cgccggatat
     7021 gttgttccag gcgctgcatc tttatcgcag caacttcaaa ccgtcggcac ggttggagaa
     7081 accgtatgcg atggtgtgca tcaatattat cgccgccgac agcaaccgcg atgccgaatt
     7141 cctgtttacc tcaatgcagc aagcctttgt gaaactgcgc cgcggcgaaa ccgggcaact
     7201 gccgccaccg attcaaaata tggatcagtt ctggtcgccg tccgaacagt atggtgtgca
     7261 gcaggcgctg agtatgtcgc tggtgggcga taaagcgaaa gtgcgtcatg gcttgcagtc
     7321 gatcctgcgc gaaaccgacg ccgatgagat tatggttaac gggcagattt tcgaccacca
     7381 ggcacgcctg cattcgtttg agctggcgat ggatgtgaag gaagagttgt tggggtagtg
     7441 tgttttaacg taggaagcct tatccgagat ggcaacgctg tcctacgtag gcctgataag
     7501 cgaagcgcat caggcattgt gtaggcagca gaaatgtcgg ataaggcacc gctgattact
     7561 gatacaccgg cagcacatta aagctcgata agatatggac cagtgcgttg ccaacgccaa
     7621 ataccagaat cagcataatc atcggcttgc cgccccatac acggaatttc gggctaccaa
     7681 agcgtttacg cgatgcgcgg gccaacagcg ccggaacaat tgccgcccag atggtagccg
     7741 ctaagccagc ataaccaatg gcgtacagga atccgttcgg gaacagcagg ccacccacaa
     7801 caggcggggc aaaggtcagc aacgctgttt tcaaacggcc catagccgag tcgtcaaaac
     7861 caaacagatc tgccagatag tcaaacaaac ccagcgttac gccaaggaac gaactcgcta
     7921 ccgcaaagtt tgagaacacg accagtagaa gatccagact acggctgttc agtacgccgc
     7981 ttaacgcctg taccagcaca tcaatattac cgcccttctc ggcgataccg ataaactccg
     8041 gacgcgggat gttacccatc gtcgccagca accagatggt atacagcgcc agcgccatca
     8101 gcgtaccgta caccaggcat ttcacgatgg ttttcggatc tttgccgtaa tacttcatca
     8161 ggctcggcac gttaccgtga taaccaaaag atgccagaca gaatggcagt gtcatcagca
     8221 gatacggcgc ataagacgca ttgctttcgg cgacgttgaa caaggtcgtt ggctgtacat
     8281 gccccagcag gctgccgaag gtgaggaaga aagtaatgac tttcgccccc agcacaattg
     8341 ctgtcatccg gctgacagct ttcgtactca accacaccac gaacgctacc agcagtgcaa
     8401 aaccaaatcc agctgcccgt gcagggacgt tcagcgacat ctccgcgaag gtgtgatgca
     8461 gaatcgaacc gctggcagaa atataagcgt aagtcaggat atagagcaca aaggcaatgg
     8521 aaatgccgtt gactacgttc cagcctttgc ccagcaaatc tttggtgatg gtgtcaaagc
     8581 tagaaccgat tcgataattc aggttagctt ccagaatcat caagccggaa tgcagcatac
     8641 agaaccaggt aaagatcagc gccgccattg accagaagaa ccacgccccg gacatgacca
     8701 caggcagaga aaacatccct gcgccaataa tggtgccgcc gataatcacc acgccgccaa
     8761 gcagcgacgg tgacgtttgg gtggtggtta gtgttgccat gagggcttct ctccagtgaa
     8821 aaatagtgcg actgtgttgt tatgcattgc actgtaccag tacatgagta caaaaaacag
     8881 aaaaaaagcc ccgatggtta aaatcggggc tgtatatatt attttacaga ttgtgttcgc
     8941 tgttcagcga tgattacgca tcaccaccga aacgacgacg accggtagag tcatcacgac
     9001 gcggtgcgcg gccttcacga cgttcgccgc taaaacgacg cccatcacca cgaccccctt
     9061 cacggcgttc accgctgaag ttacgaccgc cttcacgacg ttcgccaccg aaaccacgac
     9121 caccgccacg acgctcaccg ccagtatgcg gctgtgcatc gcccagtaac tgcatgttca
     9181 tcggcttgtt gagaatgcga gtacgcgtaa agtgttgcag cacttcaccc ggcatacctt
     9241 tcggcagttc gatggtggag tgagaagcaa acagcttgat gttaccgatg taacggctgc
     9301 tgatgtcgcc ttcgttagca atcgcgccaa cgatatgacg aacttcaaca ccatcatcgc
     9361 ggcccacttc aatacggtac aactgcatat cgccaacatc acgacgttcg cgacgcggac
     9421 gatcttcacg gtcgccacgc gggccacggt cgttacgatc gcgcggacca cggtcatcac
     9481 ggtcacggaa ttcacgtttc ggacgcatcg gcgcatctgg cggaacaatc agagtacgtt
     9541 caccctgtgc cattttcagt agtgcggcgg ccagagtttc gagatccagc tcttcacctt
     9601 cggcagtcgg ctgaattttg ctcagcagtg cacggtattg atccagatcg ctgctttcca
     9661 gctgctgctg tactttagcg gcaaattttt ccagacgacg tttgcccagc agttctgcgt
     9721 tcggcagttc aacttccgga atggtcagct tcatggtgcg ttcaatgtta cgcagcagac
     9781 gacgctcacg gttctcaacg aacagcagcg cacggccagc acggcccgca cgaccagtac
     9841 gaccgatacg gtgaacgtaa gactcagaat ccatcgggat atcgtagtta actaccaggc
     9901 tgatacgctc aacgtccagg ccacgggccg caacgtcggt cgcaatcagg atgtccagac
     9961 gaccatcttt cagtcgttcc agtgtctgct cacgcagcgc ctggttcatg tcaccgttca
    10021 gcgcggcgct gttgtagccg ttacgctcaa gggcttctgc cacttccaga gtcgcgtttt
    10081 tggtacgaac gaagataatc gccgcatcaa aatcttccgc ttccaggaaa cgtaccagtg
    10141 cttcgttttt acgcataccc cagacagtcc agtagctctg gctgatgtca ggacgggtag
    10201 tcacgctgga ctgaatgcgc acttcctgcg gctctttcat aaagcggcgg gtaatgcgac
    10261 gaatcgcttc cggcatggtt gcagagaaca gagcagtctg atgaccttcc gggatctgcg
    10321 ccataatggt ttcaacgtct tcgatgaagc ccatacgcag catttcgtca gcttcatcca
    10381 gaaccagacc gctcagttta gagaggtcca gagtgccacg tttcaggtgg tccagcagac
    10441 ggcccggagt accgacaacg atctgcggcc cctgacgcag ggcgcgtaat tgcacgtcat
    10501 aacgttggcc gccgtacagg gcaaccacgt ttacgccgcg catgtgttta gagaaatccg
    10561 tcattgcttc agcaacctgt accgccagtt cgcgggtcgg tgccagcacc agaatctgtg
    10621 gtgctttcag ctcaggatca agattctgca gcagaggtaa agagaatgct gcagtttttc
    10681 cgctccccgt ctgggccata cccaggacgt cgcggccatt cagcagatgt ggaatacact
    10741 ctgcctgaat tggagatggt ttttcgtaac ccagatcgtt aagggcttca aggataggag
    10801 ccttcaggcc cagatctgca aaagtggttt cgaattcagc catgtagtac gtgtgcctca
    10861 aaattaatgg cggccagtct acataactca tcatgaaatt gatcagcaat tttcattgaa
    10921 aagtgtgaac cggctcaaag taggtgtatt aacgaacaac aacgccctca cccgttaagg
    10981 tgatggcaat caaaaaagat tacgggctga tgtgtacgtc agctattgct ggtccgattc
    11041 tgccaggtca tcttggtcct ggcccaggag cgataattcc aacaatgcgt atcggtgctc
    11101 aacaaagtta tgaacgttgt tggcaaccgc cagtttgaac agtgccgtgg cgctgtccaa
    11161 atcccccaga cttaggtagt acttacctaa atagaagttg gtttcactga gatgctcagc
    11221 gagcgaggtg ttatccgttg cgtccgcctt gagcctttcc attaacgttt gttcgctaat
    11281 gttgcccagg tagaactcga caatgttcca tccccactgt tccttatccg atttttcgaa
    11341 gtgctgtttc aacacttctt tagcctgctt ctcatcgagc ttctgctcgg cgagataaag
    11401 ccacagacta cggaaaggat cattgggatc gtcttgataa aacgccagca gatcatcttg
    11461 cgctaacttg tcacgaccgc cgtaatataa tgcgatcccg cgattcaagt gcgcgtagtt
    11521 gtaagttgga tcaagctcaa gtacagaatc aaacgcttca taggcagcat caaaattgcc
    11581 tgcctgcgtt aaatatatgc ctaagtaatt gaatacttca ggcatatccg gtcggattgc
    11641 cagcgcttgc gaaaaatcgt tacgcgctaa tgccctcaga ccgagactat catacaacac
    11701 tccgcgctca tataaaagct gtgcgcgttc gtcatcggtt aaagcccgac tggcaaggat
    11761 ctgttccata cgtgccagaa tcacttcctg ctgtaaagtc ggttgcaatg gtaccgcgag
    11821 gacttcactt ttacgccagg aagtattact gcatcctgca agcgtaagtg ctgtcgcaac
    11881 gaaacaccag cgcaaaaaag gcttcatttc ccactcccga agaccacggt tgaatgaacg
    11941 tcctgttccc ggttgctaac aaggcgtcct gcccggttaa aagccccccg ccgcagcgga
    12001 gggcaaatgg caaccttact cgccctgttc agcagccgga gcttccggtg ctgcagcagg
    12061 ttgagactgc tcagtcgctt ctttaatgct cagacggata cggccctggc gatcaacttc
    12121 cagaactttc accggtactt cctgacccat ctgcaggtaa tcggtcactt tctcaacgcg
    12181 tttatcagcg atttgagaga tgtggaccag accttcttta ccgccaccaa tggcaacaaa
    12241 tgcgccaaaa tcaacgatac gggtcacttt accattgtag acgcggccca cttcgatttc
    12301 tgcagtgatc tcttcgatac gacgaatcgc gtgtttcgct ttctcgccgt cggtcgctgc
    12361 aatcttcaca gtaccgtcat cttcaatttc gatggtggtg ccggtttctt cggtcagagc
    12421 acggattaca gaaccgcctt taccgataac atctttgatc ttgtccgggt tgatcttgat
    12481 ggtatggata cgcggtgcga actcagagat atcgccacgc ggcgcgttga tcgcctgttc
    12541 cattaccccc aggatatgca gacgcgcacc tttagcctgg ttcagtgcaa cctgcatgat
    12601 ctctttggtg ataccttcaa ttttgatatc catctgcaat gcagagatac cgtcgcggga
    12661 acccgcaact ttgaagtcca tatcgcccag gtgatcttcg tcgcccaaaa tgtcagacag
    12721 tacaacgtag ttgtcgcctt ctttcaccag acccattgcg atacccgcaa cggcagcttt
    12781 gattggcaca cctgcgtcca tcagcgccag agacgcgccg cacacggaag ccatagaaga
    12841 agaaccgttg gattcggtga tttcagacac tacacgtacg gtgtacggga atttgtccat
    12901 atccggcatg actgccagca cgccgcgctt cgccagacga ccgtgaccaa tttcacgacg
    12961 cttcggagaa ccgaccatgc cggtttcgcc tacggagtac ggagggaagt tgtagtggaa
    13021 caggaaggtg tcagtacgtt cgcccatcag ttcatcaaga acttgcgcgt cacgtgcagt
    13081 acccagcgtt gcggtaacca gcgcctgagt ttcaccacgg gtgaaaagcg cagaaccgtg
    13141 agtacgcggc agcacgccag tacgcacatc cagaccacga atcatatctt tttcacgacc
    13201 gtcgatacgc ggttcgcctg ccagtacgcg gctacgaaca acgtttttct cgatagcgtg
    13261 cagaatttca cccagttcgt tttcgtccag ggtttcatct tcagcaagca gcgtcgcgat
    13321 ggtttcagat ttgatgacat caacctgcgc ataacgctct tgtttgtcgg tgatgcggta
    13381 agcatcgctc agacgagctt cagccagcgc agcaacgcgc gcgttcagcg cttcgtttac
    13441 cggctccggc tgccagtccc aacgcggttt accggcttct ttcaccagtt cattgatgtt
    13501 ctgaataaca acctgctgtt gttcatgacc gaacaccact gcgcccagca tttggtcttc
    13561 gctcagcagt tcagcttcag attcaaccat cagcacggca gcttctgtac cggcaacaac
    13621 caggtccagt ttgctctctt tcagttcgtc ctgagtcggg ttcagtacgt actggtcatt
    13681 gatgtaacct acacgggcag caccaatcgg gccattgaac ggaataccag acagagacag
    13741 tgctgcggaa gcaccaatca tcgcgacgat atccgggtta acctgcgggt taacagaaac
    13801 cacggtggcg ataacctgaa cttcgttgac gaagccttcc gggaacagcg ggcgaatcgg
    13861 gcggtcaatc agacgcgcga tcagggtttc gccttcgctt gggcggcctt cacgacggaa
    13921 gaagctaccc gggatacgac cagcagcgta ggtacgctcc tgatagttaa cggtcagcgg
    13981 gaagaagtcc tgacctggtt tggctttttt ctgaccaaca acggtaacaa ataccgcggt
    14041 gtcatccatg ctaaccataa cagcggcagt agcctgacga gccatcatgc cggtttccag
    14101 agtcacggtg tgttggccgt actggaattt acgaacgatc ggattaagca atgtaatatc
    14161 ctttctcttt cttagacagt accttacggt actggtgtta atacccgatc ttctgcgcat
    14221 cctcgcgact aatgacaacc ctaacccagc tctatgtggg taaagcctct cattagccgc
    14281 gcgaacctct gcaacggaag atcattcata gcaacaatac attagtttcc agtgaattgc
    14341 tgccgtcagc ttgaaaaaag gggccactca ggcccccttt tctgaaactc gcaagaatta
    14401 gcgacgcaga cccaggcgct cgatgagctg ggtgtaacgt gctacgtctt tacgtttcag
    14461 gtagtcgagc agtttacgac gctgagaaac catgcgcagc agaccacgac ggctgtggtg
    14521 atcttttttg tgctctgcaa agtggccctg caggtggttg atctgtgcag tcagcagtgc
    14581 tacctgaact tcggtagaac cggtgtcgtt tgcgtcacga ccaaactcag aaacgatttt
    14641 agctgttgct tcagtactta gagacatttt aaaactccaa agtatataga atgaaaggac
    14701 gccgatctct aattcagcga tcccagtgta cgttacgcaa agtgttaaac aatttacgcg
    14761 acgttaagcg gcagtattct actcgtagcg acctgttatc gcaagacggt taacattacg
    14821 ccgggtattc aaccaccagg cgacgaggcg caacgcggcc ttcatcgtca atttcgccca
    14881 taccgataaa cttgccgttc tcaccctctg taacgcgaac cagtccttcc agcggcgcac
    14941 cggatgtacg aactgggtta ccatttttga agtaaacaga agacgttaac ggaagattca
    15001 ccactgggta gtccgaagct ggactgtcca ttggcatcag taatggatca agtaactccg
    15061 cagccggaat atcctgctgt tcggcttgct caaccagttc acgcagatgt tccagggtca
    15121 ccatccgttc aaccggatat ttacttaccg ccagacgacg caagtaaata acatgcgcgc
    15181 cacaaccgag tttttcgccg agatcgtcaa tgatagtgcg gatataagtg ccttttgagc
    15241 agtgaatttc cagctccagc tcattgcctt catggcgaat aaacagcaat tcataaacgg
    15301 taatcgggcg cgcttcacgc ggaacttcaa tgccctgacg cgcgtattcg tacagttttt
    15361 tgccctgata tttcagtgct gaatacatcg aagggatctg ttcgatatcg ccacggaagg
    15421 tatccagcgc cgccgccagt tgctctgcac taaaggttac cggacgttct tcaacgattt
    15481 gtccgtcggc atcagaagta tcggtacgct gtccaagacg cgcaatgacc cgatagcgtt
    15541 tgtcggagtc cagcagatac tgggaaaact tcgtcgcttc cccgaggcaa attggcaaca
    15601 tgccggtcgc cagcgggtcc agcgcaccgg tatgcccggc acggttggcg ttataaatcc
    15661 gtttcacctt ttgcagcgca tcgttgctgg acatcccctg cggtttatcc agcaacaaaa
    15721 cgccgttaat gtcgcgaccg cgacgacgag gacgactcat tagtcctcct tgctgtcgtc
    15781 cgggttaaca cgacgttctt cgtcatgttt gaccacgctg gtcaccaggt ttgacatgcg
    15841 catcccttca accagagagt tgtcgtagaa gaaggtcagt tccggcacga tacgcaggcg
    15901 catcgctttc cccagcaggc tgcggatgaa accagaagct tcctgcaacg ctttgatgcc
    15961 cgctttaacc gcgtcttcat ctttgtcgtt gaggaacgtc acatatactt tggcatacgc
    16021 caggtcgcga gacatttcga caccggaaac ggtggtcatc atgcccagac gaggatcttt
    16081 aatttcacgc tgcaggatga gagcgatctc tttttgcatt tcctgcgcta cgcgctgcgg
    16141 gcgaccaaat tctttcgcca taataaattc tcctgacaaa aaaggggctg ttagcccctt
    16201 tttaaaatta atttcaggtg gaagggctgt tcacgttggc ctgataagac gcgccagcgt
    16261 cgcatcagac aatccatgca ggatgcggca taaacgcctt atcccgcatg gaaccctaaa
    16321 aaccttaagc gatggtacgt tggatctcga tgatttcgaa tacttcgatc acatcgccag
    16381 tgcggacatc gttgtagttc ttaacgccga taccacattc cataccgtta cggacttcgt
    16441 taacgtcatc tttgaagcgg cgcagggact ccagctcgcc ttcgtagata accacgttgt
    16501 cacgcagaac gcggatcggg ttgtgacgtt taaccacacc ttcggtaacc atacaacctg
    16561 cgatggcacc aaatttcggt gatttgaaca cgtcacgaac ttccgccaga ccgataatct
    16621 gctgtttcag ttccggagac agcataccgc tcatcgccgc tttcacttcg tcgatcaggt
    16681 tatagatgac ggagtagtaa cgcagatcca ggctttccgc ttcaatcact ttacgtgcag
    16741 aggcatcagc acgtacgttg aagccaacca ggatggcgtt ggacgccgca gccagggtag
    16801 cgtcggtttc ggtgatacca cctacgccag aaccaatgat cttcacttta acttcgtcag
    16861 tagacagttt cagcaaggag tcagaaattg cttccacaga accctgtacg tctgccttca
    16921 ggacgatatt cacttcgtga acttcgcctt cggtcatgtt ggcgaacatg ttctcgagtt
    16981 tagatttctg ctgacgcgcc agtttaactt cgcggaattt accctgacga tagagtgcaa
    17041 cttcacgcgc tttcttctcg tcacgtacaa cggtaacttc atcacccgca gccggtacgc
    17101 cggacaggcc aaggatttcc accggaatgg acggacccgc ttccagcact tcctgcccca
    17161 gttcgttacg cattgcacga acacgaccgt attcgaagcc acacagaacg atatcgccct
    17221 tgtgcagagt accttcacgt accagaacgg tagcaaccgg accacgacct ttatcgagga
    17281 aggattcgat aaccgcaccg ctcgccatac ctttacgtac cgctttcagc tccagcactt
    17341 ccgcctgcag caggatagca tccagcagtt catcaatacc ggtacccgct tttgcagata
    17401 cgtgtacgaa ctggctttcg ccgccccact cttctggcag gatgccgtac tgggagagtt
    17461 cgttcttaac gcgatccgga tcggcttctg gtttatcgat tttgttcaca gcaaccacca
    17521 ccggaacacc cgccgctttc gcgtgctgga tagcttcgat ggtctgcggc attacaccgt
    17581 cgtctgcagc aacaaccagg actacgatgt ccgttgcctg cgcaccacgt gcacgcattg
    17641 aagtaaacgc ggcgtgcccc ggggtgtcca ggaaggtgat catgccgttt tcagtttcaa
    17701 cgtggtatgc accaatgtgc tgggtaatgc cgcccgcttc gccagaggcc actttcgttg
    17761 aacgaatgta gtccagcaga gaggttttac cgtggtcaac gtgacccatg atggtcacaa
    17821 ccggcgcgcg cggttcagcc gcagcacccg tgtcacggtc gctcattacc gcctcttcca
    17881 gctcgttttc acgacgcagg ataactttgt ggcccatctc ttcagcaacc agctgtgcgg
    17941 tttcctgatc gataacctgg ttgatggttg ccattgcgcc cagtttcatc atcgctttga
    18001 tgacctgaga gcctttaacc gccatcttgt tcgccagttc gccaacggtg atggtttcgc
    18061 cgatcacaac gtcacggtta acggcctgag ctggcttctg gaagccttgc tgcagcgaag
    18121 aacctttacg ttttccgccc ttaccgccac gtactgctgc gcgagcttct tcacgatcag
    18181 cttttgattc agcgtgtttg ttgcctttct tcggacgcgc tgctttcgcg ttacgaccac
    18241 ggccacggcc gccttcgact tcacgatcgc tttcgtcttc tgcctggcga gcatgttgag
    18301 aagtagtgac gtgataatcg ctggaatctt cagtcggttc cgcgttatca gtccatttgt
    18361 tttcttccgc catacgacgt gcttcttcag caacgcgacg tgcttcttct tcgagtttac
    18421 gacgcgcttc ttcttcggct ttacgcttga gctctgcagc ttcctgctca cggcgggctt
    18481 tttcagcctg ggcgttttta gtcatatcgt cttgttgatt gctcactttg tctttttccg
    18541 cagcttcacg tttcgcttgt tcagcagctt cacgcttagc ttgttctgcg gcctcacgtt
    18601 cagctttttg ttgcgcctcg cgtttagccg attcttctgc ctcacgacgg gcttgctctt
    18661 ccgcttcacg ctgcgcttgc tcttccgctg caaggcgttc agcctcttgc ggatcgcgtt
    18721 tcacaaaggt gcgtttcttg cggacttcga tttgtaccga tttgcttttt ccaccggtac
    18781 caggaatgtt aagggtgctg cgtgttttac gttgcagcgt caatttgtcc gggcctgaat
    18841 ttttctgatt caggtggtca atcaaagtct gtttctcttg tgcagacaca gagtcgtcag
    18901 cagacttccg gatacctgca tcagcaaatt gctgtaccag gcgttccacg gaggtctgtc
    18961 gctctgcggc cagcgtttta atcgttacat ctgtcatgct gttccttcct gctacagttt
    19021 attacgcttc gtcaccgaac cagcaaatat tacgggcagc cataatcagt gctccggctt
    19081 tttcgtcggt caacccttcg atatcagcca gatcatcaat gccctgttcg gcgagatctt
    19141 ccagcgtaca aacgccacgg gcggccagtt tgaatgccaa atcacgatct accccttcaa
    19201 ggttcagcag atcgtcagcc ggtttgttat caccgaggct ttcttcctgg gcctgtgcaa
    19261 tggtggccag tgcattttta gcacgctcgc gcagtgcttc aacggtcggc tcatcaaggc
    19321 cttcgatttc caacagctct ttcatcggca cataggccag ttcttccagc gtcgagaaac
    19381 cttcttctac cagaacagtc gcgaagtctt cgtcgatgtc gagatatttg gtgaaggtgt
    19441 cgatcgctgc gtgcgcttcc gcctgatgct tagcctgcag gtcgtcaacg gtcattacgt
    19501 tgagttccca accgctcagc tgcgaagcca gacgcacgtt ctgaccgtta cggccaatcg
    19561 cctgcgccag gttaccggct tcaacggcga tatccatggt gtgtttatct tcatccacca
    19621 cgatagaagc aacgtctgcc ggtgccattg cgttaatcac aaactgcgcc gggttatcat
    19681 cccacaggac gatatcgata cgctcgccgc ccagttcagt agaaaccgcc tgaacacgcg
    19741 cgccacgcat acctacgcaa gcacctaccg gatcgatacg tttatcgttg gttttcaccg
    19801 cgattttcgc acgagaaccc gggtcgcgag ccgctgcttt aatttcaatc acttcttcgc
    19861 cgatttctgg cacttcaata cggaacagtt cgatcagcat ttccggcttg gaacgagtga
    19921 cgaacagttg cgcgccacgc gcttccgggc gaacggaata aagcacgcca cgaacgcggt
    19981 cgccagggcg gaagttttca cgcggcagca tatcttcgcg caggatcacg gcttcagcgt
    20041 tgttgcccag atccagagag atgttgtcgc ggtttacttt tttcaccacg ccggtgatga
    20101 tttcaccttc gtgttcacgg aactgatcaa ccaccatcgc acgttcggct tcacgcactt
    20161 tctgcacgat aacctgtttt gccgtctggg tggtgatacg gtcaaaggta acagactcaa
    20221 tctgatcttc aacgtaatcg cccaagttca ggctttcatc ttcataacgt gcggcttcaa
    20281 gggtgatttc cttggtcggc tgggtgactt catcaacaac taaccagcga cggaaggtgt
    20341 caaaatcacc gcttttgcga tcgatctgta cgcggacgtc gatctcttgt tcatattttt
    20401 tctttgttgc tgtcgccagc gcgctttcca atgcttcgaa aatcttctcg cgaggtagcg
    20461 ccttttcatt ggatacggct tcaactacag ccaaaatttc tttgttcatc gcgggctttt
    20521 cacctcatcc agactattaa aagtggggaa ccaggttcgc cttctggata ttactcagcg
    20581 cgaacacttc atctttacct tcgacggtaa ctgtgatcat ttcaccgtct accgctttga
    20641 taacgccctg ccatttacga cggttttgta ccgccatacg gagaaccaga gtcacctctt
    20701 ctccgacaaa acgggcgtag tgttcagccg tgaacagtgg gcgatcgaga cccggtgagg
    20761 agacttccag gttataagca acggtgatgg gatcttcaac atccagcaca gcacttacct
    20821 ggtggctcac atcagcacaa tcatcaacat tgatgccatc ttcactatca atatagatgc
    20881 gcagtgtgga tgtgcgaccg cgaataaatt cgatgccaac cagttcaaaa cccagggcct
    20941 caactggcgc agtaatcatc tctgttaatt tttgctctaa tgtggacaag cccaccccca
    21001 agacataaaa aaagggccta aagcccagtt attctgtagt cagataacaa aaaaccccga
    21061 taaatcgggg ctttatataa ctgaacccta taaccgcaac tgcggtctgg agcactttcc
    21121 agaaggattt tttcaaatcc cactacgaag gccgaagtct tcacagtata tttgaaaaag
    21181 gactctaagg gaaa
//
LOCUS       DADSYM010000078        21118 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2415
            SAMN08039579-rid11723173.denovo.078, whole genome shotgun sequence.
ACCESSION   DADSYM010000078 DADSYM010000000
VERSION     DADSYM010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039579
            Sequence Read Archive: SRR6362533
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 21118)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21118)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980162.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 03:39:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,484
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 4,347
            CDSs (with protein)               :: 4,347
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 43 of 85
            Pseudo Genes (incomplete)         :: 37 of 85
            Pseudo Genes (internal stop)      :: 19 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21118
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039579-rid11723173.denovo.078"
                     /strain="LGE2415"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8302 N 95.9628 W"
                     /collection_date="2015-08-24"
     gene            147..1157
                     /gene="glpX"
                     /locus_tag="J1Y83_002537"
     CDS             147..1157
                     /gene="glpX"
                     /locus_tag="J1Y83_002537"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HBA7630679.1"
                     /translation="MRRELAIEFSRVTESAALAGYKWLGRGDKNTADGAAVNAMRIML
                     NQVNIDGTIVIGEGEIDEAPMLYIGEKVGTGRGDAVDIAVDPIEGTRMTAMGQANALA
                     VLAVGDKGCFLNAPDMYMEKLIVGPGAKGTIDLNLPLADNLRNVAAALGKPLSELTVT
                     ILAKPRHDAVIAEMQQLGVRVFAIPDGDVAASILTCMPDSEVDVLYGIGGAPEGVVSA
                     AVIRALDGDMNGRLLARHDVKGDNEENRRIGEQELARCKAMGIEAGKVLRLGDMARSD
                     NVIFSATGITKGDLLEGISRKGNIATTETLLIRGKSRTIRRIQSIHYLDRKDPEMQVH
                     IL"
     gene            1254..2000
                     /gene="fpr"
                     /locus_tag="J1Y83_002538"
     CDS             1254..2000
                     /gene="fpr"
                     /locus_tag="J1Y83_002538"
                     /EC_number="1.18.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin--NADP(+) reductase"
                     /protein_id="HBA7630680.1"
                     /translation="MADWVTGKVTKVQNWTDALFSLTVHAPVLPFTAGQFTKLGLEIE
                     GERVQRAYSYVNSPDNPDLEFYLVTVPDGKLSPRLAALKPGDEVQVVSEAAGFFVLDE
                     VPDCETLWMLATGTAIGPYLSILQLGKDLERFKNMVLVHAARYAADLSYLPLMQELEK
                     RYEGKLRIQTVVSRETAAGSLTGRIPALIESGELESAVGLPMNKETSHVMLCGNPQMV
                     RDTQQLLKETRQMTKHLRRRPGHMTAEHYW"
     gene            complement(2005..2433)
                     /gene="uspD"
                     /locus_tag="J1Y83_002539"
     CDS             complement(2005..2433)
                     /gene="uspD"
                     /locus_tag="J1Y83_002539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspD"
                     /protein_id="HBA7630681.1"
                     /translation="MAYKHIGVAISGNEEDALLVNKALELARHNDAHLTLIHIDDGLS
                     ELYPGIYFPATEDILQLLKNKSDNKLYKLTKNIQWPKTKLRIERGEMPETLLEIMQKE
                     QCDLLVCGHHHSFINRLMPAYRGMINKLSADLLIVPFIDK"
     gene            complement(2460..2759)
                     /locus_tag="J1Y83_002540"
     CDS             complement(2460..2759)
                     /locus_tag="J1Y83_002540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF406 domain-containing protein"
                     /protein_id="HBA7630682.1"
                     /translation="MKDVVDKCSTKGCAIDIGTVIDNDNCTSKFSRFFATREEAESFM
                     TKLKELAAAASSADEGASVAYKIKDLEGQVELDAAFTFSCQAEMIIFELSLRSLA"
     gene            complement(2971..3411)
                     /locus_tag="J1Y83_002541"
     CDS             complement(2971..3411)
                     /locus_tag="J1Y83_002541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HBA7630683.1"
                     /translation="MTIQQWLFSFKGRIGRRDFWIWIGLWFAGMLVLFSLAGKNLLDI
                     QTAAFCLVCLLWPTAAVTVKRLHDRGRSGAWAFLMIVAWMLLAGNWAILPGVWQWAVG
                     RFIPTLILVMMLIDLGAFVGTQGENKYGKDTQDVKYKADNKSSN"
     gene            3512..4111
                     /locus_tag="J1Y83_002542"
     CDS             3512..4111
                     /locus_tag="J1Y83_002542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiQ family protein"
                     /protein_id="HBA7630684.1"
                     /translation="MKPGCTLFFLLCSALTVTTTAHAQTPDTATTAPYLLAGAPTFDL
                     SISQFREDFNSQNPSLPLNEFRAIDSSPDKANLTRAASKINENLYASTALERGTLKIK
                     SIQITWLPIQGPEQKAAKAKAQEYMAAVIRTLTPLMTKTQSQKKLQSLLTAGKNKRYY
                     TETEGALRYVVADNGEKGLTFAVEPIKLALSESLEGLNK"
     gene            4219..4986
                     /gene="tpiA"
                     /locus_tag="J1Y83_002543"
     CDS             4219..4986
                     /gene="tpiA"
                     /locus_tag="J1Y83_002543"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HBA7630685.1"
                     /translation="MRHPLVMGNWKLNGSRHMVHELVSNLRKELAGVAGCAVAIAPPE
                     MYIDMAKREAEGSHIMLGAQNVDLNLSGAFTGETSAAMLKDIGAQYIIIGHSERRTYH
                     KESDELIAKKFAVLKEQGLTPVLCIGETEAENEAGKTEEVCARQIDAVLKTQGAAAFE
                     GAVIAYEPVWAIGTGKSATPAQAQAVHKFIRDHIAKVDANIAEQVIIQYGGSVNASNA
                     AELFAQPDIDGALVGGASLKADAFAVIVKAAEAAKQA"
     gene            complement(5041..5796)
                     /gene="cdh"
                     /locus_tag="J1Y83_002544"
     CDS             complement(5041..5796)
                     /gene="cdh"
                     /locus_tag="J1Y83_002544"
                     /EC_number="3.6.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol diphosphatase"
                     /protein_id="HBA7630686.1"
                     /translation="MKKAGLLFLVMIVIAVVAAGIGYWRLTGEESDTLRKIVLEQCLP
                     NQQENQNPSPCAEVKPNAGYVVLKDRHGPLQYLLMPTYRINGTESPLLTDPSTPNFFW
                     LAWQARDFMSQKYGQPVPDRAVSLAINSRTGRTQNHFHIHISCIRPDVREQLDNNLAN
                     ISSRWLPLPGGLRGHEYLARRVTESELVQRSPFMMLAEEVPEAREHMGSYGLAMVRQS
                     DNSFVLLATQRNLLTLNRASAEEIQDHQCEILR"
     gene            complement(5903..6892)
                     /locus_tag="J1Y83_002545"
     CDS             complement(5903..6892)
                     /locus_tag="J1Y83_002545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter substrate-binding
                     protein"
                     /protein_id="HBA7630687.1"
                     /translation="MKKWGVGLTFLLAATSVMAKDIQLLNVSYDPTRELYEQYNKAFS
                     AHWKQQTGDNVVIRQSHGGSGKQATSVINGIEADVVTLALAYDVDAIAERGRIDKEWI
                     KRLPDNSAPYTSTIVFLVRKGNPKQIHDWNDLIKPGVSVITPNPKSSGGARWNYLAAW
                     GYALHHNNNDQAKAQDFVRALYKNVEVLDSGARGSTNTFVERGIGDVLIAWENEALLA
                     ANELGKDKFEIVTPSESILAEPTVSVVDKVVEKKGTKEVAEAYLKYLYSPEGQEIAAK
                     NYYRPRDAEVAKKYENAFPKLKLFTIDEEFGGWTKAQKEHFANGGTFDQISKR"
     gene            complement(7212..8174)
                     /gene="pfkA"
                     /locus_tag="J1Y83_002546"
     CDS             complement(7212..8174)
                     /gene="pfkA"
                     /locus_tag="J1Y83_002546"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HBA7630688.1"
                     /translation="MIKKIGVLTSGGDAPGMNAAIRGVVRSALTEGLEVMGIYDGYLG
                     LYEDRMVQLDRYSVSDMINRGGTFLGSARFPEFRDENIRAVAIENLKKRGIDALVVIG
                     GDGSYMGAMRLTEMGFPCIGLPGTIDNDIKGTDYTIGFFTALSTVVEAIDRLRDTSSS
                     HQRISVVEVMGRYCGDLTLAAAIAGGCEFVVVPEVEFSREDLVNEIKAGIAKGKKHAI
                     VAITEHMCDVDELAHFIEKETGRETRATVLGHIQRGGSPVPYDRILASRMGAYAIDLL
                     LAGYGGRCVGIQNEQLVHHDIIDAIENMKRPFKGDWLDCAKKLY"
     gene            complement(8355..9257)
                     /gene="fieF"
                     /locus_tag="J1Y83_002547"
     CDS             complement(8355..9257)
                     /gene="fieF"
                     /locus_tag="J1Y83_002547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709720.2"
                     /note="FieF, a metal efflux transporter, is a member of
                     the CDF (cation diffusion facilitator) family of
                     transporters; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family cation-efflux transporter FieF"
                     /protein_id="HBA7630689.1"
                     /translation="MNQSYGRLVSRAAIAATAMASLLLLIKIFAWWYTGSVSILAALV
                     DSLVDIGASLTNLLVVRYSLQPADDNHSFGHGKAESLAALAQSMFISGSALFLFLTGI
                     QHLVSPTPMTDPGVGVIVTIVALICTIILVSFQRWVVRRTQSQAVRADMLHYQSDVMM
                     NGAILLALGLSWYGWHRADALFALGIGIYILYSALRMGYEAVQSLLDRALPDEERQEI
                     IDIVTSWPGVSGAHDLRTRQSGPTRFIQIHLEMEDSLPLVQAHMVADQVEQAILRRFP
                     GSDVIIHQDPCSVVPREGKRSMLS"
     gene            complement(9406..9906)
                     /gene="cpxP"
                     /locus_tag="J1Y83_002548"
     CDS             complement(9406..9906)
                     /gene="cpxP"
                     /locus_tag="J1Y83_002548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell-envelope stress modulator CpxP"
                     /protein_id="HBA7630690.1"
                     /translation="MRIVTAAVMASTLAVSSLSHAAEVGSGDNWHPGEELTQRSTQSH
                     MFDGISLTEHQRQQMRDLMQQARHEQPPVNVSELETMHRLVTAENFDENAVRAQAEKM
                     ANEQIARQVEMAKVRNQMYRLLTPEQQAVLNEKHQQRMEQLRDVTQWQKSSSLKLLSS
                     SNSRSQ"
     gene            10056..10754
                     /gene="cpxR"
                     /locus_tag="J1Y83_002549"
     CDS             10056..10754
                     /gene="cpxR"
                     /locus_tag="J1Y83_002549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001033718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response regulator transcription
                     factor CpxR"
                     /protein_id="HBA7630691.1"
                     /translation="MNKILLVDDDRELTSLLKELLEMEGFNVIVAHDGEQALDLLDDS
                     IDLLLLDVMMPKKNGIDTLKALRQTHQTPVIMLTARGSELDRVLGLELGADDYLPKPF
                     NDRELVARIRAILRRSHWSEQQQNNDNGSPTLEVDALSLNPGRQEATFDGQTLELTGT
                     EFTLLYLLAQHLGQVVSREHLSQEVLGKRLTPFDRAIDMHISNLRRKLPDRKDGHPWF
                     KTLRGRGYLMVSAS"
     gene            10751..12124
                     /gene="cpxA"
                     /locus_tag="J1Y83_002550"
     CDS             10751..12124
                     /gene="cpxA"
                     /locus_tag="J1Y83_002550"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress sensor histidine kinase CpxA"
                     /protein_id="HBA7630692.1"
                     /translation="MIGSLTARIFAIFWLTLALVLMLVLMLPKLDSRQMTELLDSEQR
                     QGLMIEQHVEAELANDPPNDLMWWRRLFRAIDKWAPPGQRLLLVTTEGRVIGAERSEM
                     QIIRNFIGQADNADHPQKKKYGRVELVGPFSVRDGEDNYQLYLIRPASSSQSDFINLL
                     FDRPLLLLIVTMLVSTPLLLWLAWSLAKPARKLKNAADEVAQGNLRQHPELEAGPQEF
                     LAAGASFNQMVTALERMMTSQQRLLSDISHELRTPLTRLQLGTALLRRRSGESKELER
                     IETEAQRLDSMINDLLVMSRNQQKNALVSETIKANQLWSEVLDNAAFEAEQMGKSLTV
                     NFPPGPWPLYGNPNALESALENIVRNALRYSHTKIEVGFAVDKDGITITVDDDGPGVS
                     PEDREQIFRPFYRTDEARDRESGGTGLGLAIVETAIQQHRGWVKAEDSPLGGLRLVIW
                     LPLYKRS"
     gene            complement(12295..12969)
                     /gene="yiiM"
                     /locus_tag="J1Y83_002551"
     CDS             complement(12295..12969)
                     /gene="yiiM"
                     /locus_tag="J1Y83_002551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-N-hydroxylaminopurine resistance protein"
                     /protein_id="HBA7630693.1"
                     /translation="MRYPVDVYTGKIQAYPEGKPSAIAKIQVDGELMLTDLGLEGDEQ
                     AEKKIHGGPDRALCHYPREHYLYWAREFPEQAELFVAPAFGENLSTDGLTESNVYIGD
                     IFRWGEALIQVSQPRSPCYKLNYHFDISDIAQLMQNTGKVGWLYSVIAPGLVSADAPL
                     ELVSRVSDVTVQEAAAIAWHMPFDDDQYHRLLSAAGLSKSWTRTMQKRRLSGKLEDFS
                     RRLWGK"
     gene            complement(13118..14101)
                     /gene="kdgT"
                     /locus_tag="J1Y83_002552"
     CDS             complement(13118..14101)
                     /gene="kdgT"
                     /locus_tag="J1Y83_002552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-3-deoxygluconate transporter"
                     /protein_id="HBA7630694.1"
                     /translation="MQIKRSIEKIPGGMMLVPLFLGALCHTFSPGAGKYFGSFTNGMI
                     TGTVPILAVWFFCMGASIKLSATGTVLRKSGTLVVTKIAVAWVVAAIASRIIPEHGVE
                     VGFFAGLSTLALVAAMDMTNGGLYASIMQQYGTKEEAGAFVLMSLESGPLMTMIILGT
                     AGIASFEPHVFVGAVLPFLVGFALGNLDPELREFFSKAVQTLIPFFAFALGNTIDLTV
                     IAQTGLLGILLGVAVIIVTGIPLIIADKLIGGGDGTAGIAASSSAGAAVATPVLIAEM
                     VPAFKPMAPAATSLVATAVIVTSILVPILTSIWSRKVKARAAKIEILGTVK"
     gene            complement(14361..14981)
                     /gene="sodA"
                     /locus_tag="J1Y83_002553"
     CDS             complement(14361..14981)
                     /gene="sodA"
                     /locus_tag="J1Y83_002553"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010345169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Mn]"
                     /protein_id="HBA7630695.1"
                     /translation="MSYTLPSLPYAYDALEPHFDKQTMEIHHTKHHQTYVNNANAALE
                     NLPEFANLPVEELITKLDQLPADKKTVLRNNAGGHANHSLFWKGLKKGTTLQGDLKAA
                     IERDFGSVDNFKAEFEKAAASRFGSGWAWLVQKGDKLAVVSTANQDSPLMGEAISGAS
                     GFPILGLDVWEHAYYLKFQNRRPDYIKEFWNVVNWDEVAARFAAKK"
     gene            15267..16301
                     /gene="rhaT"
                     /locus_tag="J1Y83_002554"
     CDS             15267..16301
                     /gene="rhaT"
                     /locus_tag="J1Y83_002554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose/proton symporter RhaT"
                     /protein_id="HBA7630696.1"
                     /translation="MSNAITMGIFWHLIGAASAACFYAPFKKVKKWSWETMWSVGGIV
                     SWIILPWAISALLLPNFWAYYSSFSLSTLLPVFLFGAMWGIGNINYGLTMRYLGMSMG
                     IGIAIGITLIVGTLMTPIINGNFDVLINTEGGRMTLLGVLVALIGVGIVTRAGQLKER
                     KMGIKAEEFNLKKGLVLAVMCGIFSAGMSFAMNAAKPMHEAAAALGVDPLYVALPSYV
                     VIMGGGAIINLGFCFIRLAKVKDLSLKADFSLAKPLIIHNVLLSALGGLMWYLQFFFY
                     AWGHARIPAQYDYISWMLHMSFYVLCGGIVGLVLKEWNNAGRRPVTVLSLGCVVIIVA
                     ANIVGIGMAN"
     gene            complement(16298..17236)
                     /gene="rhaR"
                     /locus_tag="J1Y83_002555"
     CDS             complement(16298..17236)
                     /gene="rhaR"
                     /locus_tag="J1Y83_002555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709710.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator RhaR"
                     /protein_id="HBA7630697.1"
                     /translation="MVFCNNANLLNVFVRHIANNQLRSLAEVATVAHQLKLLKDDFFA
                     SDQQAVAVADRYPQDVFAEHTHDFCELVIVWRGNGLHVLNDRPYRITRGDLFYIHADD
                     KHSYASVNDLVLQNIIYCPERLKLNLDWQGAIPGFSASAGQPHWRLGSVGMAQARQVI
                     GQLEHESSQHVSFANEMAELLFGQLVMLLNRHRYTSDSLPPTSSETLLDKLITRLAAS
                     LKSPFALDKFCDEASCSERVLRQQFRQQTGMTINQYLRQVRVCHAQYLLQHSRLLISD
                     ISTECGFEDSNYFSVVFTRETGMTPSQWRHLNSQKD"
     gene            complement(17220..18056)
                     /gene="rhaS"
                     /locus_tag="J1Y83_002556"
     CDS             complement(17220..18056)
                     /gene="rhaS"
                     /locus_tag="J1Y83_002556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator RhaS"
                     /protein_id="HBA7630698.1"
                     /translation="MTVLHSVDFFPSGNASVAIEPRLPQADFPEHHHDFHEIVIVEHG
                     TGIHVFNGQPYTITGGTVCFVRDHDRHLYEHTDNLCLTNVLYRSPDRFQFLAGLNQLL
                     PQEQDGQYPSHWRVNHSVLQQVRQLVAQMEQQEGENDLPSTASREILFMQLLLLLRKS
                     SLQENLENSASRLNLLLAWLEDHFADEVNWDAVADQFSLSLRTLHRQLKQQTGLTPQR
                     YLNRLRLMKARHLLRHSEASVTDIAYRCGFSDSNHFSTLFRREFNWSPRDIRQGRDGF
                     LQ"
     gene            18344..19813
                     /gene="rhaB"
                     /locus_tag="J1Y83_002557"
     CDS             18344..19813
                     /gene="rhaB"
                     /locus_tag="J1Y83_002557"
                     /EC_number="2.7.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase"
                     /protein_id="HBA7630699.1"
                     /translation="MTFRNCVAVDLGASSGRVMLARYERECRSLTLREIHRFNNGLHS
                     QNGYVTWNVDSLESAIRLGLNKVCEEGIRIDSIGIDTWGVDFVLLDQQGQRVGLPVAY
                     RDSRTNGLMAQAQQQLGKRDIYQRSGIQFLPFNTIYQLRALTEQQPELIPHIAHALLI
                     PDYFSYRLTGKMNWEYTNATTTQLVNINSDDWDESLLAWSGANKAWFGRPTHPGNVIG
                     HWICPQGNEIPVVAIASHDTASAVIASPLNGSRAAYLSSGTWSLMGFESQTPFTNDTA
                     LAANITNEGGAEGRYRVLKNIMGLWLLQRVLQERQINDLPALIAATQALPACRFIINP
                     NDDRFINPDEMCSEIQAACRETAQPIPESDAELARCIFDSLALLYADVLHELAQLRGE
                     DFSQLHIVGGGCQNTLLNQLCADACGIRVIAGPVEASTLGNIGIQLMTLDELNNVDDF
                     RQVVSTTANLTTFTPNPDSEIAHYVAQIHSTRQTKELCA"
     gene            19810..21069
                     /gene="rhaA"
                     /locus_tag="J1Y83_002558"
     CDS             19810..21069
                     /gene="rhaA"
                     /locus_tag="J1Y83_002558"
                     /EC_number="5.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose isomerase"
                     /protein_id="HBA7630700.1"
                     /translation="MTTQLEQAWELAKQRFAAVGIDVEEALRQLDRLPVSMHCWQGDD
                     VSGFENPEGLLTGGIQATGNYPGKARNASELRADLEQAMRLIPGPKRLNLHAIYLESD
                     TPVSRDQIKPEHFKNWVEWAKANQLGLDFNPSCFSHPLSADGFTLSHADDRIRQFWID
                     HCKASRRVSAYFGEQLGTPSVMNIWIPDGMKDITVDRLAPRQRLLAALDEVISEKLNP
                     AHHIDAVESKLFGIGAESYTVGSNEFYLGYATSRQTALCLDAGHFHPTEVISDKISAA
                     MLYVPQLLLHVSRPVRWDSDHVVLLDDETQAIASEIVRHDLFDRVHIGLDFFDASINR
                     IAAWVIGTRNMKKALLRALLEPTAELRKLESAGDYTARLALLEEQKSLPWQAVWEMYC
                     QRHDTPAGSEWLENVRTYEKEILSRRG"
BASE COUNT         5268 a         5494 c         5322 g         5034 t
ORIGIN      
        1 gcgcgtctta tcaggcctac gagtcggata cacatccggc atcacacaga ctctgaaacc
       61 gtgcgggggc aaccccgcac acatcaataa tcccgccctt cccctgtgct acacttcgcg
      121 ccattcctta ctgcttagag tttgctatga gacgagaact tgccatcgaa ttttcccgcg
      181 tcaccgaatc agcagcgctg gctggctaca aatggttagg acgcggcgat aaaaacaccg
      241 cggacggcgc ggcggtaaac gccatgcgta ttatgctcaa ccaggtcaac attgacggca
      301 ccatcgttat tggtgaaggt gaaatcgacg aagcaccgat gctctacatt ggtgaaaaag
      361 tcggtactgg tcgcggcgac gcggtagata ttgctgttga tccgattgaa ggcacgcgca
      421 tgacggcgat gggccaggct aacgcgctgg cggtgctggc agtaggcgat aaaggctgct
      481 tcctcaacgc gccagatatg tatatggaga agctgatcgt cggaccggga gccaaaggca
      541 ccattgatct gaacctgccg ctggcggata acctgcgcaa tgttgcggcg gcgctcggca
      601 aaccgttgag cgaactgacg gtaacgattc tggcaaaacc gcgccacgat gccgttatcg
      661 cagaaatgca gcaactcggc gtgcgcgtat ttgctattcc ggacggcgac gttgcagcct
      721 caattctcac ctgtatgcca gacagcgaag ttgacgtgct gtacggtatt ggtggcgcgc
      781 cggaaggcgt ggtttctgcg gcggtgatcc gcgcattaga tggtgacatg aacggtcgtc
      841 tgctggcgcg tcatgacgtc aaaggcgaca acgaagagaa tcgtcgcatt ggcgagcagg
      901 agctggcacg ctgcaaagcg atgggcatcg aagccggtaa agtattgcgt ttgggcgata
      961 tggcgcgcag cgacaacgtc atcttttctg ccaccggcat caccaaaggt gatctgctgg
     1021 aaggtattag ccgcaaaggc aatatcgcca ccaccgaaac gctgctgatc cgtggtaaat
     1081 cacgcaccat tcgtcgcatt cagtccatcc actatctgga tcgtaaagac ccggaaatgc
     1141 aggtgcacat tctctgagtg atttgatcga ttgagccttc cagtccttcg ggactggaat
     1201 ttttttgttc ggagaacgaa gataaggcaa gtcaatcaaa acaggagaaa aacatggctg
     1261 attgggtaac aggcaaagtc actaaagtgc agaactggac cgacgccctg tttagtctca
     1321 ccgttcacgc tcccgtgctt ccgtttaccg ccgggcaatt caccaaactt ggccttgaaa
     1381 ttgaaggcga acgcgttcag cgcgcctact cctatgttaa ctcgcccgat aatccggatc
     1441 tggagtttta cctggtcacc gtccccgatg gcaaattaag tccacgactg gcggcactga
     1501 aaccgggcga tgaagtgcag gtggttagcg aagccgctgg cttctttgtg ctggatgaag
     1561 taccagattg cgaaacgcta tggatgctgg caaccggtac agcgattggc ccttatttat
     1621 cgattctgca attgggcaaa gatttagaac gcttcaaaaa catggtgctg gttcacgccg
     1681 cacgatatgc cgccgactta agctatctac ctctgatgca ggaactggaa aaacgctacg
     1741 aggggaaact gcgcattcag acggtggtca gtcgggaaac ggctgcgggt tcgctcactg
     1801 ggcgaatacc ggcgttaatt gaaagcggcg aactggaaag cgcggttggc ctgccgatga
     1861 ataaagaaac cagccatgtg atgctgtgcg gcaatccaca gatggtgcgc gatacacaac
     1921 agttgctgaa ggagacccgg cagatgacga aacatttacg tcgccgaccg ggccatatga
     1981 cagcggagca ttactggtaa gcggttactt gtcgataaac ggcacgatca agagatccgc
     2041 actcaactta ttgatcatcc cgcgatatgc cggcatcaaa cggttgataa atgagtggtg
     2101 gtgaccacag acaaggaggt cgcactgctc tttttgcata atttccagca gtgtttccgg
     2161 catttctccg cgttcaatac gcagttttgt cttcggccat tgaatatttt tcgtcagttt
     2221 atacagcttg ttatccgact tattcttcaa caattgaaga atatcttctg ttgcagggaa
     2281 gtagataccc ggatacaact cgcttaagcc atcatcaata tgaattaacg tcaggtgagc
     2341 gtcattatgt ctggcgagct ccagggcttt attcaccagt aaggcatctt cttcattccc
     2401 ggaaattgcc acgccaatgt gtttataagc catatttaac tccttctaaa gcaactccat
     2461 caagctaacg aacgcagtga taactcaaaa ataatcatct cggcctggca tgagaaggtg
     2521 aaggccgcat caagctcaac ttgcccctcc agatccttaa tcttataggc cacgctggcc
     2581 ccttcatctg cagaggatgc agcggcggca agctctttca gtttggtcat aaaggactct
     2641 gcttcttcgc gggtggcaaa aaagcgcgaa aacttactgg tacagttgtc gttatcaatc
     2701 accgtaccga tatcaatcgc acatccttta gtactgcatt tatctacgac atctttcata
     2761 gggcacctct gtatattcgc ctctctgtcg attacgactt aagaataatg cgccgttaaa
     2821 ggaagaacaa acaagtaatg atcttatgga ttaccatttt ttttgatatt gcttaaggtg
     2881 aagagttcag aaggattttc cgggcattgt cacttgcatc gtggaaataa caatcccgga
     2941 aaaaaatagc gataagagat atattaaagg ttaattactt gatttattgt cggctttata
     3001 cttcacatcc tgggtatctt taccatattt attttcgcct tgtgtgccaa caaacgcacc
     3061 aagatcgata agcatcatca ccagaatcaa tgtcgggata aaacgcccca ccgcccattg
     3121 ccagacaccc ggtaaaattg cccagttacc cgccagcagc atccacgcca caatcatcag
     3181 aaatgcccat gcgccggaac gcccgcgatc atgcaagcgc ttaacggtta ctgccgctgt
     3241 tggccaaagc aagcacacaa ggcaaaacgc cgcggtctga atatcgagta aatttttacc
     3301 cgccagtgag aaaagcacca gcatgcctgc gaaccacagg cctatccaaa tccagaaatc
     3361 acggcgtcca atacgccctt taaatgagaa taaccattgc tgtatggtca tgtaagttcc
     3421 ttgatggttg tcttttccag gattctaccc ttttgacaag ggcgacagat atcgttttaa
     3481 tcggagccag tcataacaaa aggtactgtc aatgaagcca gggtgtacgc tgttttttct
     3541 cttatgttct gcattaaccg ttacaacaac ggcgcatgca caaacaccag atacggcaac
     3601 gaccgcgcct tatctgctgg ctggagcccc tactttcgat ctctccatca gccagtttcg
     3661 agaagacttt aacagccaga atcccagcct gccactgaac gaatttcgtg ccatcgacag
     3721 cagtcccgac aaagccaatc tcactcgtgc agcaagtaaa attaacgaga acttgtatgc
     3781 ttctactgcg ctggagcgcg gtaccttaaa aatcaaaagc attcaaataa cctggctacc
     3841 catccagggg ccagagcaaa aagccgcgaa agcgaaagct caggaataca tggcagcggt
     3901 gatccgcaca ctcaccccat taatgaccaa aactcaaagc cagaaaaaac tgcagtcgct
     3961 actaacggcg gggaaaaaca aacgttatta caccgagaca gaaggtgcac tgcgttatgt
     4021 tgtcgcggac aacggcgaaa aggggctgac cttcgctgtt gaaccgatta agctggcgct
     4081 atctgaatcg cttgaaggtt tgaataaatg acaaaaagca aagcctttgt gccgatgaat
     4141 ctctatactg tttcacagac ctgctgccct gcggggcggc catcttcctt tattcgctta
     4201 taagcgtgga gaattaaaat gcgacatcct ttagtgatgg gtaactggaa actgaacggc
     4261 agccgccaca tggttcacga gctggtatct aacctgcgta aagagctggc aggtgttgct
     4321 ggctgtgcgg ttgcaatcgc accaccggaa atgtacatcg atatggcgaa gcgtgaagct
     4381 gaaggcagcc acatcatgct gggtgcgcaa aacgtggacc tgaacctgtc cggcgcattc
     4441 accggtgaaa cctctgctgc tatgctgaaa gacatcggcg cacagtacat catcatcggc
     4501 cactctgaac gtcgtactta ccacaaagaa tctgacgaac tgatcgcgaa aaaattcgcg
     4561 gtgctgaaag agcagggact gactccggtt ctgtgcatcg gtgaaaccga agcggaaaac
     4621 gaagcgggca aaactgaaga agtttgtgca cgtcagatcg acgcggtact gaaaactcag
     4681 ggtgctgcgg cattcgaagg tgcggttatc gcttacgaac ctgtatgggc aatcggtact
     4741 ggcaaatctg caactccggc tcaggcacag gctgttcaca aattcatccg tgaccacatt
     4801 gctaaagttg acgctaacat cgctgaacaa gtgatcattc agtacggcgg ctctgtaaac
     4861 gcgtctaacg ctgcagaact gtttgctcag ccagacatcg acggcgcgct ggttggcggt
     4921 gcttctctga aagctgacgc tttcgccgtg atcgttaaag ctgcagaagc ggctaaacag
     4981 gcttaagtct gacaggtgcc ggatttcata tccggcactt actttcctaa actcctcgcc
     5041 ttaacgcaaa atctcacact gatgatcctg gatttcttcg gctgaagcac ggttcagcgt
     5101 cagtagattt cgttgtgtcg ccagcaatac aaatgagtta tcactctgcc gtaccatcgc
     5161 cagcccgtag cttcccatat gttcccgcgc ctcaggcact tcttccgcca gcatcataaa
     5221 tgggctgcgt tgtaccagtt cgctttccgt tacccgacgc gccaggtact catgcccacg
     5281 caaaccacct ggcagtggca accagcggct actgatgttc gccagattgt tatccagctg
     5341 ttcgcgcaca tcaggacgaa tacaagaaat atgaatatga aaatggtttt gcgtacgccc
     5401 ggtgcgggag ttgatcgcca gagataccgc gcgatcggga acaggctggc cgtatttttg
     5461 gctcataaaa tcacgcgcct gccaggctaa ccagaaaaaa ttaggcgttg acggatcggt
     5521 caacaatggg ctttcagtac cgttaatacg gtacgttggc attaacagat attgcagtgg
     5581 gccatgacga tcttttaaaa ccacgtatcc ggcgttgggt ttgacttccg cacatggcga
     5641 cgggttttgg ttttcctgct ggttgggcaa acattgctca agaactatct tacgtaacgt
     5701 gtctgactct tcaccggtta atctccagta accaataccg gcagccacaa cggcgataac
     5761 tatcatcacc aaaaaaagaa gacccgcttt tttcatcttt ttttccctgt acctcaaaga
     5821 gatgcaaggg taacgcaaaa tcgagacaaa taaaaaaccc ggtcgcgaga aaccaccggg
     5881 tttgataatt atcctggaga aatcagcgtt tgctgatctg atcgaacgta ccgccgttag
     5941 caaaatgctc tttttgcgct ttcgtccagc cgccgaactc ttcatcaatg gtgaataact
     6001 tcaattttgg aaacgcattt tcgtactttt tcgctacctc agcgtcgcgc ggacggtagt
     6061 agtttttcgc tgcaatttcc tgaccttctg gcgagtagag atatttcagg taggcttccg
     6121 ccacctcttt ggtgcctttt ttctcgacca ctttatcgac caccgacacg gttggctctg
     6181 cgaggataga ctcactcggc gtaacgattt cgaatttatc tttccccagt tcattcgctg
     6241 ccagcagagc ttcattttcc caggcaatca gtacatcgcc aattccgcgc tcgacaaaag
     6301 tgttagtgga tccgcgcgcg ccagaatcca gaacttcgac gtttttatac agtgcccgaa
     6361 caaaatcctg tgcttttgcc tgatcgttgt tgttgtgatg cagcgcgtag ccccaggctg
     6421 ccaggtaatt ccagcgcgcg ccaccagagc ttttcggatt aggcgtgatc accgaaacac
     6481 ccggtttaat cagatcgttc cagtcatgga tctgcttcgg attaccctta cgtaccagga
     6541 aaacaatggt ggaagtgtac ggtgcggagt tatccggcag acgtttgatc cactctttat
     6601 caatccgccc gcgttccgca attgcgtcca cgtcataggc cagagccaga gtgacaacat
     6661 cagcttcaat accgttgatt accgacgtcg cttgtttgcc tgaaccaccg tgtgactgac
     6721 gaatcaccac gttatcgcca gtttgctgtt tccagtgggc gctgaatgcc ttgttgtact
     6781 gttcgtacaa ttcgcgcgtt ggatcatatg aaacgttaag aagctgaata tcctttgcca
     6841 taacgctggt tgccgccagc aaaaatgtta accctacgcc ccactttttc atcgcccgac
     6901 tctcttatgt tgtgttgtga tgagcaaagc gtgccagaag gttgaccaaa cattaaagaa
     6961 taaaaaaaga ttggctataa cttgcgagta tatgttgggg gattaaaaag gcggaagaaa
     7021 tccgcctcat attgctgaca aagtgcgctt tgtccatgcc ggatgcggca caaacgcctt
     7081 atccggccta caaaagtttg caaattcaat agattacaga attcatgtag gcctgataag
     7141 cgaagcgcat caggcctttt tgcttctgtc atcggtttca ggctaaagga atctgccttt
     7201 ttccgaaatc attaatacag ttttttcgcg cagtccagcc agtcgccttt gaacggacgc
     7261 ttcatgtttt cgatagcgtc gatgatgtcg tggtgaacca gctgttcgtt ctggatacct
     7321 acgcaacgac caccgtaacc tgccagcagc agatcgatag cgtaagcgcc catacgggaa
     7381 gccagaatac ggtcgtaagg caccggagaa ccaccgcgct ggatgtggcc cagcacagtt
     7441 gcgcgggttt cacgaccggt ttctttctcg atgaaatgcg ccagttcgtc aacatcacac
     7501 atatgttcgg taatcgccac gatcgcgtgt tttttacctt tcgcgatacc cgctttgatt
     7561 tcgtttacca ggtcttcacg gctgaattca acttccggaa ccacaacgaa ttcacagcca
     7621 ccggcaatgg ccgcagccaa cgtcagatcg ccacaataac ggcccatcac ttccaccacg
     7681 gaaatacgct ggtgagaaga agaggtgtca cgcagacggt cgatcgcttc tacaacggtg
     7741 ctcagcgcag tgaagaaacc gatagtgtag tcagtgcctt tgatgtcgtt gtcgatagtg
     7801 cccggcaggc cgatgcacgg gaagcccatt tcggtcagac gcattgcacc catgtaggaa
     7861 ccgtcaccgc cgataaccac cagcgcgtcg atcccacgtt ttttcaggtt ttcgatagcc
     7921 acggcgcgga tgttctcgtc gcggaattcc gggaaacgcg cagaaccgag gaacgtacca
     7981 ccacggttga tcatgtcaga aacgctgtaa cggtctagct gtaccatacg gtcttcatac
     8041 agacccagat agccgtcata aatgcccatt acttccagac cttctgtcag cgcagaacga
     8101 acaaccccgc gaattgcggc gttcatgcct ggcgcatcac cgccgcttgt caacacaccg
     8161 attttcttaa tcatgactac ctctgaactt tggaatgcaa aatgaaatct gttgccggaa
     8221 gtcttcttgc acatcgaagt gatccaacga atgtgcaaat agtataacaa tcacttcctg
     8281 ctgaattgat tcaggtcagg ccaaatggcg gtattttata cacaaaatgc gggtctggct
     8341 ctcttttata ctgattatga aagcatagac cgtttaccct ccctgggtac gacggaacag
     8401 gggtcctgat ggataatcac atccgatccc ggaaaacgcc gtaaaatagc ctgctctacc
     8461 tgatccgcca ccatatgtgc ctgaaccaaa ggcagagagt cttccatttc caaatgaatc
     8521 tgaataaagc gggtcggccc tgactgccgc gtgcgaagat cgtgagcgcc gctaacaccc
     8581 ggccaggaag tcacgatatc aataatttct tgccgttcct catcaggcaa tgcgcgatcc
     8641 agtaatgact gtaccgcctc atatcccatg cgtaacgcgc tatataaaat atagatgccg
     8701 atccccaatg caaacagagc atcggcgcga tgccagccgt accaggacaa ccccagcgcc
     8761 agcagaattg cgccgttcat cataacatca gactggtaat gtagcatatc agcccgcacc
     8821 gcctggcttt gcgtccggcg caccacccaa cgctgaaacg agacaaggat aatcgtacaa
     8881 attagcgcca caattgtcac gataaccccg acgcctggat ctgtcatcgg tgttggagat
     8941 accagatgtt gaatacccgt caaaaacagg aatagtgccg aaccggagat aaacatactt
     9001 tgcgccagcg ccgcgaggga ctcagcttta ccgtgaccaa acgagtgatt atcgtcggca
     9061 ggttgcaggg aatatcgcac caccagtaaa ttcgtcaacg acgcgccgat atccaccagc
     9121 gaatccacca gcgcggcgag aatactcacc gacccggtat accaccatgc aaaaatttta
     9181 atcagcaata gcagcgaagc catcgccgtc gcagcaatcg ccgcccgact gaccagccgt
     9241 ccataagatt gattcataaa tactcccgct atcaactgac ggtagtataa cggaagcaaa
     9301 tcatctgcaa tgcattaagc agcaggcaaa ttgaggataa aaaaaacccc cacatcatgt
     9361 gggggaagac agggatggtg tctatggcaa ggaaaacagg gtttactact gggaacgtga
     9421 gttgctacta ctcaatagct tcaacgatga acttttttgc cattgcgtca cgtcgcgcaa
     9481 ctgctccatt cgttgttgat gtttctcgtt taaaaccgct tgctgctccg gcgttaacag
     9541 gcgatacatt tggttgcgga cttttgccat ctcaacctga cgagcaattt gctcattcgc
     9601 cattttttct gcctgtgcgc gcacagcgtt ttcatcaaaa ttttctgcgg tgacaaggcg
     9661 atgcattgtc tccagttcgc taacattaac aggaggctgt tcgtgccggg cctgttgcat
     9721 aagatctcgc atctgctgac gctgatgttc ggttaaactt atgccgtcga acatatggct
     9781 ctgcgtactg cgctgcgtaa gttcttcacc cggatgccag ttatcgcctg aaccgacttc
     9841 agcagcgtgg cttaatgaac tgactgccag cgttgaggcc atgacggcag cggtaactat
     9901 gcgcatcatt tgctcccaaa atctttctgt cgcgattcaa cgatagagag tttacgattc
     9961 aggctgcaaa catgcgtcag ggggtgtaaa acaacgtaaa gtcatggatt agcgacgtct
    10021 gatgacgtaa tttctgcctc ggaggtattt aaacaatgaa taaaatcctg ttagttgatg
    10081 atgaccgaga gctgacttcc ctattaaagg agctgctcga gatggaaggc ttcaacgtga
    10141 ttgttgccca cgatggggaa caggcgcttg atcttctgga cgacagcatt gatttacttt
    10201 tgcttgatgt aatgatgccg aagaaaaatg gtatcgacac attaaaagca cttcgccaga
    10261 cacaccagac gcctgtcatt atgttgacgg cgcgcggcag tgaacttgat cgcgttctcg
    10321 ggcttgagct gggcgcagat gactatctcc caaaaccgtt taatgatcgt gagctggtgg
    10381 cacgtattcg cgcgatcctg cgccgttcgc actggagcga acaacagcaa aacaacgaca
    10441 acggttcacc gacactggaa gttgatgcct taagtctcaa tcctggtcgc caggaagcca
    10501 ccttcgacgg gcaaacgctg gagttaaccg gtacggagtt taccctgctc tatttgctgg
    10561 cacagcatct gggtcaggtg gtttcccgtg agcatttaag ccaggaagtg ctgggcaagc
    10621 gcctgacgcc tttcgaccgc gctatcgata tgcacatttc caacctgcgt cgtaaactgc
    10681 cggatcgtaa agatggtcac ccgtggttta aaaccttgcg tggtcgcggc tatctgatgg
    10741 tttctgcttc atgataggca gcttaaccgc gcgcatcttc gccatcttct ggctgacgct
    10801 ggcgctggtg ttgatgttgg ttttgatgtt acccaagctc gattcacgcc agatgaccga
    10861 gcttctggat agcgaacagc gtcagggtct gatgattgag cagcatgtcg aagcggagct
    10921 ggcgaacgat ccgcccaatg atttaatgtg gtggcggcgt ctgttccggg cgattgataa
    10981 gtgggcaccg ccaggacagc gtttgttatt ggtgaccacc gaaggccgcg tgatcggcgc
    11041 tgaacgcagc gaaatgcaga tcattcgtaa ctttattggt caggccgata acgccgatca
    11101 tccgcagaag aaaaagtatg gccgcgtgga actggtcggt ccgttctccg tgcgtgatgg
    11161 cgaagataat taccaacttt atctgattcg tccggccagc agttctcaat ccgatttcat
    11221 taacttactg tttgaccgcc cgctattact gctgattgtc accatgttgg tcagtacgcc
    11281 gctgctgttg tggttggcct ggagtctggc aaaaccggcg cgtaagctga aaaacgctgc
    11341 cgatgaagtt gcccagggaa acttacgcca gcacccggaa ctggaagcgg ggccacagga
    11401 attccttgcc gccggtgcca gttttaacca gatggtcacc gcgctggagc gcatgatgac
    11461 ctcccagcag cgtctgcttt ctgatatctc tcacgagctg cgcaccccgc tgacgcgtct
    11521 gcaactgggt acggcgttac tgcgccgtcg tagcggtgaa agcaaagaac tggagcgtat
    11581 tgaaaccgaa gcgcaacgtc tggacagcat gattaacgac ctgttggtga tgtcacgtaa
    11641 tcagcagaaa aacgcgctgg ttagcgagac catcaaagcc aatcagttgt ggagtgaagt
    11701 gctggataac gcggcgttcg aagccgagca aatgggcaag tcgttgacag ttaacttccc
    11761 gcctgggccg tggccgctgt acggcaaccc gaacgctctg gagagtgcgc tggaaaacat
    11821 tgttcgtaat gccctgcgtt attcccatac gaagattgaa gtgggctttg cggtagataa
    11881 agacggtatc accattacgg tggacgacga tggtcctggc gttagcccgg aagatcgcga
    11941 acagattttc cgtccgttct atcggaccga tgaagcacgc gatcgtgaat ctggcggtac
    12001 aggtttgggg ctggcgattg ttgaaaccgc cattcagcag catcgtggct gggtgaaggc
    12061 agaagacagc ccgctgggcg gtttacggct ggtgatttgg ttgccgctgt ataagcggtc
    12121 ataacgtcaa cattcaggta aaaaaatgcc tgatgcacta cgcttatcag gcctacaaaa
    12181 cctgttgaat ttatgggttt tgtaggcagg ataaggcgtt tacgccgcat ccggcatttg
    12241 agcaggatgc ctgatgcgac gctgatagcg tcttatcagg cctacactcc ggggttattt
    12301 tccccacaaa cggcgggaaa aatcttcgag cttgccgctc agtcggcgct tttgcatcgt
    12361 tcgcgtccag cttttcgata accctgcggc ggagagtaaa cggtgatact gatcgtcatc
    12421 aaacggcata tgccatgcga tggcggcagc ctcctgcacg gtcacatcgc tgacacggga
    12481 aaccaactcc agcggcgcat cagcagatac caaccctggt gcaattacgc tatacaacca
    12541 ccccacttta cccgtgtttt gcatcaactg cgctatatcg ctgatatcaa aatggtaatt
    12601 gagtttatag caaggtgagc gtggctggct gacctgaatt aatgcctctc cccagcggaa
    12661 aatatcacca atataaacat tactttccgt cagaccgtcg gttgagagat tttcgccaaa
    12721 cgcaggcgca acaaacagct ccgcctgttc cggaaattcc cgcgcccagt agagataatg
    12781 ctcacgagga taatgacaca gcgctctgtc tggcccgccg tggatctttt tctccgcctg
    12841 ttcgtcacct tccagcccca ggtccgtcag catcaactca ccatcaacct ggattttggc
    12901 aattgcgctg ggtttgcctt cgggataagc ctgaatcttg cctgtgtata catcaaccgg
    12961 atatcgcatc tgttccctct ccacgagaaa tttccctatt agaccatatt tattttatgt
    13021 caggcgtgct gttttcgcgg taagtatcca ggcagaaacg caattgaaaa ccggacgaac
    13081 gttagttcat ccggctgtgg ggattaagga agctggttta tttcaccgta cctaaaattt
    13141 cgattttcgc cgctctggct ttgactttac gtgaccagat agaggtaaga attggcacca
    13201 gaatcgaagt cacaatgacc gccgtcgcga ccagcgaagt tgctgccgga gccataggtt
    13261 taaacgcagg caccatttca gcaatcagca caggtgtcgc taccgcggcc cctgcggaac
    13321 tggaagcggc aattccggca gtgccatcgc caccgccaat caatttatcg gcgataatca
    13381 acggaatacc ggtcacgata attactgcca cacccaacag gatccccagc aaacctgtct
    13441 gggcaattac agtcaaatca atggtattgc ccagtgcgaa ggcaaagaat ggaataagcg
    13501 tctgcaccgc tttgctgaaa aattctcgta actcagggtc aaggttcccc agggcaaagc
    13561 ccaccaggaa cggcaatact gcgccgacga aaacatgcgg ttcaaacgag gcaatcccgg
    13621 cagtgcccag aataatcatc gtcatgagcg gcccggactc caacgacatc aacacaaatg
    13681 ccccagcttc ttcttttgtg ccgtactgct gcatgatgga agcgtaaagt ccgccgttgg
    13741 tcatatccat cgccgccacc agcgccaacg ttgaaagtcc ggcaaaaaat ccaacttcaa
    13801 caccatgttc cggaataatg cgcgaggcaa ttgccgcaac cacccacgcg acggcaattt
    13861 tagttaccac cagcgtaccg gatttacgca gtaccgttcc cgtcgcgctt aattttattg
    13921 acgcccccat gcaaaaaaac cacaccgcca gaatgggcac cgtaccggta atcatcccgt
    13981 tggtgaatga tccaaaatat ttccccgccc ccggcgagaa ggtgtggcac agtgcgccaa
    14041 ggaatagcgg gacgagcatc atcccccccg ggattttctc aatcgagcgt tttatctgca
    14101 tttccatcac ctgtcggcat atttttaagg gctgatgcgt cattcctcaa cggagcatca
    14161 gtgttgatgg aaacgaagat agacattcat ttgatcaata aaagtgatca aaaccacacc
    14221 aattgaaacg ctgttttatt tttatcggat cattgttttt attttgaaat tgatcacaaa
    14281 aaaaccaccg ccgttggcga tggttcattt tttaagctgc aatgcggcgt aaacgcctca
    14341 ttgcagcagg cggcaaatga ttatttttta gccgcgaaac gtgccgctac ttcgtcccag
    14401 ttcaccacgt tccagaactc tttaatgtag tccggacggc ggttctggaa tttcagatag
    14461 taagcatgtt cccacacatc caggcccaga atcgggaagc cggaagcgcc agaaatggct
    14521 tcgcccatca gcggagagtc ctggttagca gtagaaacca ccgccagttt atcgcctttt
    14581 tgcaccagcc atgcccagcc ggaaccaaag cgggaagctg ccgctttttc aaattctgct
    14641 ttgaaattat caacggaacc gaaatcacgt tcgatagccg ctttcaggtc gccctgcagg
    14701 gtggtgcctt ttttcagacc tttccagaac agactgtggt tagcgtgacc accagcgtta
    14761 ttgcgcagta cggttttctt gtctgctggc agctggtcca gtttggtgat cagctcttca
    14821 accggcaggt tggcaaattc tggcaggttt tccagcgccg cgttggcgtt gttgacgtaa
    14881 gtctgatggt gtttggtgtg gtggatttcc atggtctgct tatcgaagtg cggttccagg
    14941 gcatcgtaag catacggcag ggatggcagg gtataactca tattcatctc cagtgttgtc
    15001 gggcggccga ttgttaatgc cgcgtaagca gttggttcat tatagttaat taaatgatat
    15061 tgaaaatgat tatcaatgcc gtacttttca taagggtatg gttttgcagg aaaatgccca
    15121 agatgtgaag caaatcaccc acttaatgcc gtgattgcca gtaaatcggc agcggcggca
    15181 acaggcgaaa ggttaatcga ccgctcaatt tttacactca tctcgtcgga gatgtgacgc
    15241 gacgaaaaaa tgatgaggat aagaagatga gtaacgcgat tacgatgggg atattttggc
    15301 atttgatcgg cgcggccagt gcagcctgtt tttacgctcc gttcaaaaaa gtaaaaaaat
    15361 ggtcatggga aaccatgtgg tcagtcggtg ggattgtttc gtggattatc ctgccgtggg
    15421 ccatcagcgc cctgttacta ccgaatttct gggcgtatta cagctcgttt agtctctcta
    15481 cgctgctgcc tgtttttctg ttcggcgcta tgtgggggat cggtaatatc aactacggcc
    15541 tgaccatgcg ttatctcggc atgtcgatgg gaattggcat cgccattggc attacgttga
    15601 ttgtcggtac gctgatgacg ccaattatca acggcaattt cgatgtgctg attaataccg
    15661 aaggcggacg catgacgttg ctcggcgttc tggtggcgct gattggcgta gggattgtaa
    15721 ctcgcgccgg gcagttgaaa gagcgcaaga tgggcattaa agccgaagag ttcaatctga
    15781 aaaaagggct ggtgctggcg gtgatgtgcg gcattttctc tgccgggatg tcctttgcga
    15841 tgaacgccgc aaaaccgatg catgaagccg ctgccgcgct gggcgtcgat cccctgtatg
    15901 tcgctctgcc aagctacgtt gtcatcatgg gcggcggcgc aatcatcaac ctcggcttct
    15961 gcttcattcg tctggcaaaa gtgaaagatt tgtcgctaaa agccgacttc tcgctggcaa
    16021 aaccgctgat cattcacaat gtgttactct cagcactggg cgggttgatg tggtatctgc
    16081 aattcttttt ctatgcctgg ggccacgccc gcattccggc gcagtatgac tacatcagct
    16141 ggatgctgca tatgagcttc tatgtattgt gcggcggtat cgttgggctg gtgctgaaag
    16201 agtggaacaa tgcaggacgc cgtccggtaa cggtgttgag cctcggttgt gtggtgatta
    16261 ttgtcgccgc taacatcgtc ggcatcggca tggcgaatta atctttctgc gaattgagat
    16321 gacgccactg gctgggcgtc atcccggttt cccgggtaaa caccaccgaa aaatagttac
    16381 tatcttcaaa gccacattcg gtcgaaatat cactgattaa caggcggcta tgctggagga
    16441 gatattgcgc atgacacact ctgacctgtc gcagatattg attgatggtc attccagtct
    16501 gctgacgaaa ttgctgacgc aaaacgcgct cactgcacga tgcctcatca caaaatttat
    16561 ccagcgcaaa gggacttttc aggctagccg ccagccgggt aatcagctta tccagcaacg
    16621 tttcgctgga tgttggcggc aacgaatcac tggtgtaacg atggcgattc agcaacatca
    16681 ccaactgccc gaacagcaac tcagccattt cgttagcaaa cgacacatgc tgactacttt
    16741 catgctcaag ctgaccgata acctgccgcg cctgcgccat ccccacgcta cctaagcgcc
    16801 agtgtggttg ccctgcgctg gcgctaaatc ccggaatcgc tccctgccag tcaagattca
    16861 gcttcagccg ctccgggcaa taaataatat tctgcaaaac cagatcgtta acggaagcgt
    16921 aggagtgttt atcgtcagca tgaatataaa agagatcgcc acgggtaatg cgataagggc
    16981 gatcgttgag tacatgcaga ccattaccgc gccagacaat caccagctca caaaaatcat
    17041 gtgtatgttc ggcaaagaca tcttgcggat aacggtcagc cacagcgact gcctgctggt
    17101 cgctggcaaa aaaatcatct ttgagaagtt ttaactgatg cgccaccgtg gctacctcgg
    17161 ccagagaacg aagttgatta ttcgcaatat ggcgtacaaa tacgttgaga agattcgcgt
    17221 tattgcagaa aaccatcccg tccctgacga atatcacgcg gtgaccagtt aaactctcgg
    17281 cgaaaaagcg tcgaaaagtg gttactgtcg ctgaatccgc agcgataggc gatgtcagta
    17341 acgctggcct cgctgtggcg tagcagatgt cgggctttca tcagtcgcag gcggttcagg
    17401 taacgctgag gcgtcagccc cgtttgctgc ttaagctggc gatgcagcgt acgcaatgaa
    17461 agagaaaatt gatccgccac agcgtcccaa ttcacctcat cggcaaaatg gtcctccagc
    17521 caggccagaa gcaagttgag acgtgatgcg ctgttttcca ggttctcctg caaactgctt
    17581 ttacgcagca agagcaataa ttgcataaat aagatctcgc gactggcggt cgagggtaaa
    17641 tcattttccc cttcctgctg ttccatctgt gcaaccagct gtcgcacctg ctgcaatacg
    17701 ctgtggttaa cgcgccagtg agacggatac tgcccatcct gctcttgtgg cagcaactga
    17761 ttcagcccgg cgagaaactg aaatcgatcc ggcgagcgat acagcacatt ggtcagacac
    17821 agattatcgg tatgttcata cagatgccga tcatgatcgc gtacgaaaca gaccgtccca
    17881 ccggtgatgg tatagggctg cccattaaat acatgaatac ccgtgccatg ttcgacaatc
    17941 acaatttcat gaaaatcatg atgatgttca ggaaaatccg cctgcgggag ccggggttct
    18001 atcgccacgg acgcgttacc agacggaaaa aaatccacac tatgtaatac ggtcatactg
    18061 gcctcctgat gtcgtcaaca cggcgaaata gtaatcacga ggtcaggttc ttaccttaaa
    18121 ttttcgacgg aaaaccacgt aaaaaacgtc gatttttcaa gatacagcgt gaattttcag
    18181 gaaatgcggt gagcatcaca tcaccacaat gaagcaaatt gtgaacatca tcacgttcat
    18241 ctttccctgg ttgccaatgg cccattttcc tgtcagtaac gagaaggtca cgaattcagg
    18301 cgctttttag actggtcgta atgaaattca gcaggatcac attatgacct ttcgcaattg
    18361 tgtcgccgtc gatctcggcg catccagtgg gcgcgtgatg ctggcgcgtt acgagcgtga
    18421 atgccgcagc ctgacgctgc gcgaaatcca tcgttttaac aatggcctgc atagccagaa
    18481 cggttatgtc acctggaatg tggatagcct ggaaagtgcc attcgccttg gattaaacaa
    18541 ggtgtgcgag gaagggattc gtatcgatag cattgggatt gatacctggg gcgtggactt
    18601 tgtgctgctc gaccaacagg gtcagcgtgt gggcctgccc gttgcttatc gcgatagccg
    18661 caccaatggc ctaatggcgc aggcacaaca acaactcggc aaacgcgata tttatcaacg
    18721 tagcggcatc cagtttctgc ccttcaatac gatctatcag ctacgtgcac tgacagagca
    18781 gcaaccagaa cttattccac acattgctca cgctttgctg ataccggatt actttagtta
    18841 tcgcctgacc ggcaagatga actgggaata taccaacgcc acgaccacgc aactggtcaa
    18901 tatcaatagc gacgactggg acgagtcgct actggcgtgg agcggtgcca acaaagcctg
    18961 gtttggtcgc ccgacgcatc cgggtaatgt cataggtcac tggatttgcc cgcagggtaa
    19021 tgagattccg gtggtcgcca ttgccagcca tgataccgcc agcgcggtta tcgcctcgcc
    19081 gttaaacggt tcacgcgccg cttatctctc ttctggcacc tggtcattga tgggcttcga
    19141 aagccagacg ccatttacca atgacacggc gctggcagcc aatatcacca acgaaggcgg
    19201 ggcggaaggt cgctatcggg tgctgaaaaa tattatgggc ttatggctac ttcagcgagt
    19261 gcttcaggag cggcaaatca acgatcttcc ggcgcttatc gccgcaacac aggcacttcc
    19321 ggcctgccgc ttcatcatca atcccaatga cgatcgcttt attaatcctg acgagatgtg
    19381 cagcgaaatt caggctgcgt gtcgggaaac ggcgcaaccg atcccggaaa gtgatgctga
    19441 actggcgcgc tgtattttcg acagtctggc gctgctgtat gccgatgtgt tgcatgagct
    19501 ggcgcagctg cgtggtgaag atttctcgca actgcatatt gtcggcggcg gctgccagaa
    19561 cacgctgctc aaccagctgt gtgccgatgc ctgcggtatt cgggtgatcg cagggcctgt
    19621 tgaagcctcg acgctcggca atatcggcat ccagttaatg acgctggatg aactcaacaa
    19681 tgtggatgat ttccgtcagg tcgtcagcac taccgcgaat ctgaccacct ttacccctaa
    19741 tcctgacagt gaaattgccc actatgtggc gcagattcac tctacacgac agacaaagga
    19801 gctttgcgca tgaccactca actggaacag gcctgggagc ttgcgaaaca gcgtttcgcg
    19861 gcagtaggga ttgatgtcga ggaggcactg cgccaacttg atcgtttacc cgtttcaatg
    19921 cactgctggc agggcgatga tgtttccggt tttgaaaacc cggaaggttt gctgaccggt
    19981 ggtattcagg ctactggtaa ttatccgggc aaagcgcgta atgccagtga gctacgtgcc
    20041 gatctggaac aggctatgcg gctgattcca gggccgaaac ggcttaattt acatgccatc
    20101 tatctggaat cagacacgcc agtctcgcgc gaccagatca aaccagagca cttcaaaaac
    20161 tgggttgaat gggcgaaagc caatcagctc ggtctggatt ttaacccctc ctgtttttcg
    20221 catccgctaa gcgccgatgg ctttacgctt tcccatgccg acgaccgcat tcgccagttc
    20281 tggattgacc actgtaaagc cagccgccgt gtttcagcct attttggcga gcaactcggc
    20341 acaccatcgg tgatgaacat ctggatcccg gatggtatga aagatatcac cgttgaccgt
    20401 ctcgccccac gccagcgttt gctggcagca ctggatgaag tgatcagcga gaagctaaac
    20461 cctgcgcacc atatcgacgc cgttgagagc aaattgtttg gcattggcgc ggagagctac
    20521 acggttggct ccaatgagtt ttacctgggg tatgccacca gccgccagac ggcgctgtgc
    20581 ctggacgccg ggcatttcca cccgactgaa gtgatttccg acaagatttc cgccgccatg
    20641 ctgtatgtgc cgcagttgct gctgcacgtc agccgtccgg ttcgctggga cagcgatcac
    20701 gtagtgctgc tggatgatga aacccaggcg attgccagtg agattgttcg tcacgatctg
    20761 tttgaccggg tgcatatcgg cctcgacttc ttcgatgcct ctatcaaccg cattgccgcg
    20821 tgggtcattg gtacacgcaa tatgaaaaaa gccctgctac gtgcgttact ggaacctacc
    20881 gctgaactac gcaagctaga atcggcgggc gattacactg cgcgtctggc gctactggaa
    20941 gagcagaaat cgttgccgtg gcaggcggta tgggaaatgt attgtcagcg tcacgatacg
    21001 ccagcgggta gcgaatggct ggagaacgtg cggacttatg agaaagaaat tttgagtcgc
    21061 cgcgggtaaa cactgccgga tgcggcacaa gtgccttatc cggcctacag gtcggcaa
//
LOCUS       DADSYM010000079        21050 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2415
            SAMN08039579-rid11723173.denovo.079, whole genome shotgun sequence.
ACCESSION   DADSYM010000079 DADSYM010000000
VERSION     DADSYM010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039579
            Sequence Read Archive: SRR6362533
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 21050)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21050)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980162.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 03:39:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,484
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 4,347
            CDSs (with protein)               :: 4,347
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 43 of 85
            Pseudo Genes (incomplete)         :: 37 of 85
            Pseudo Genes (internal stop)      :: 19 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21050
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039579-rid11723173.denovo.079"
                     /strain="LGE2415"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8302 N 95.9628 W"
                     /collection_date="2015-08-24"
     gene            257..1375
                     /locus_tag="J1Y83_002559"
     CDS             257..1375
                     /locus_tag="J1Y83_002559"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HBA7630701.1"
                     /translation="MPLPDFHVSEPFTLGIELEMQVVNPPGYDLSQDSSMLIDTVKNQ
                     ITAGEVKHDITESMLELATDVCRDINQAAGQFSAMQKVVLQAAADHHLEICGGGTHPF
                     QKWQRQEVCDNERYQRTLENFGYLIQQATVFGQHVHVGCASGDDAIYLLHGLSRFVPH
                     FIALSAASPYMQGTDTRFASSRPNIFSAFPDNGPMPWVSNWQQFEALFRCLSYTTMID
                     SIKDLHWDIRPSPHFGTVEVRVMDTPLTLSHAVNMAGLIQATAHWLLTERPFKHQEKD
                     YLMYKFNRFQACRYGLEGVITDPHTGDRRSLTEATLRLLEKIAPSAHKIGASSAIEAL
                     HRQVVSGLNEAQLMRDFVADGGSLIGLVKKHCEIWAGE"
     gene            1441..1689
                     /locus_tag="J1Y83_002560"
     CDS             1441..1689
                     /locus_tag="J1Y83_002560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1158 domain-containing protein"
                     /protein_id="HBA7630702.1"
                     /translation="MKHPLETLTTAAGILLMAFLSCLLLPAPALGLTLAQKLVTTFHL
                     MDLSQLYTVLFCLWFLVLGAIEYFVLRFIWRRWFSLAD"
     gene            1754..2137
                     /locus_tag="J1Y83_002561"
     CDS             1754..2137
                     /locus_tag="J1Y83_002561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000360941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HBA7630703.1"
                     /translation="MDKQSLHETAKRLALELPFVELCWPFGPEFDVFKIGGKIFMLSS
                     ELCGVPFINLKSDPQKSLLNQQIYPSIKPGYHMNKKHWISVYPGDEISEALLHDLIND
                     SWNLVIDGLAKRDQKRLRPDSTVNQ"
     gene            2216..2869
                     /gene="nfsB"
                     /locus_tag="J1Y83_002562"
     CDS             2216..2869
                     /gene="nfsB"
                     /locus_tag="J1Y83_002562"
                     /EC_number="1.5.1.34"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-insensitive NAD(P)H nitroreductase"
                     /protein_id="HBA7630704.1"
                     /translation="MDIISVALKRHSTKAFDASKKLTPEQAEQIKTLLQYSPSSTNSQ
                     PWHFIVASTEEGKARVAKSAADNYVFNERKMLDASHVVVFCAKTAMDDAWLKLVVDQE
                     DADGRFATPEAKAANDKGRKFFADMHRKDLHDDAEWMAKQVYLNVGNFLLGVAALGLD
                     AVPIEGFDAAILDEEFGLKEKGYTSLVVVPVGHHSVEDFNATLPKSRLPQNITLTEV"
     gene            2977..4224
                     /locus_tag="J1Y83_002563"
     CDS             2977..4224
                     /locus_tag="J1Y83_002563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HBA7630705.1"
                     /translation="MQDLISQVEDLAGIEIDHTTSMVMIFGIIFLTAVVVHIFLHWVV
                     LRTFEKRAIASSRLWLQIITQNKLFHRLAFTLQGIIVNIQAVFWLQKGTEAADILTTC
                     AQLWIMMYALLSVFSLLDVILNLAQKFPAASQLPLKGIFQGIKLIGAILVGILMISLL
                     IGQSPAILISGLGAMAAVLMLVFKDPILGLVAGIQLSANDMLKLGDWLEMPKYGADGA
                     VIDIGLTTVKVRNWDNTITTIPTWSLVSDSFKNWSGMSASGGRRIKRSISIDVTSIRF
                     LDEDEMQRLNKAHLLKPYLTSRHQEINEWNRQQGSTESVLNLRRMTNIGTFRAYLNEY
                     LRNHPRIRKDMTLMVRQLAPGDNGLPLEIYAFTNTVVWLEYESIQADIFDHIFAIVEE
                     FGLRLHQSPTGNDIRSLAGAFKQ"
     gene            complement(4383..5765)
                     /gene="pheP"
                     /locus_tag="J1Y83_002564"
     CDS             complement(4383..5765)
                     /gene="pheP"
                     /locus_tag="J1Y83_002564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016237767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine transporter"
                     /protein_id="HBA7630706.1"
                     /translation="MKNASTVSEDTASNQEPTLHRGLHNRHIQLIALGGAIGTGLFLG
                     IGPAIQMAGPAVLLGYGVAGIIAFLIMRQLGEMVVEEPVSGSFAHFAYKYWGPFAGFL
                     SGWNYWVMFVLVGMAELTAAGIYMQYWFPDVPTWIWAAAFFIIINAVNLVNVRLYGET
                     EFWFALIKVLAIIGMIGFGLWLLFSGHGGEKASIDNLWRYGGFFATGWNGLILSLAVI
                     MFSFGGLELIGITAAEARDPEKSIPKAVNQVVYRILLFYIGSLVVLLALYPWVEVKSN
                     SSPFVMIFHNLDSNVVASTLNFVILVASLSVYNSGVYSNSRMLFGLSVQGNAPKFLTR
                     VSRRGVPINSLMLSGAITSLVVLINYLLPQKAFGLLMALVVATLLLNWIMICLAHLRF
                     RAAMRRQGRETQFKALLYPFGNYLCIAFLGMILLLMCTMDDMRLSAILLPVWIVFLFV
                     AFKTLRRKVN"
     gene            complement(5867..9010)
                     /gene="cusA"
                     /locus_tag="J1Y83_002565"
     CDS             complement(5867..9010)
                     /gene="cusA"
                     /locus_tag="J1Y83_002565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter permease
                     subunit CusA"
                     /protein_id="HBA7630707.1"
                     /translation="MIEWIIRRSVANRFLVLMGALFLSIWGTWTIINTPVDALPDLSD
                     VQVIIKTSYPGQAPQIVENQVTYPLTTTMLSVPGAKTVRGFSQFGDSYVYVIFEDGTD
                     PYWARSRVLEYLNQVQGKLPAGVSAELGPDATGVGWIYEYALVDRSGKHDLADLRSLQ
                     DWFLKYELKTIPDVAEVASVGGVVKEYQVVIDPQRLAQYGISLAEVKSALDASNQEAG
                     GSSIELAEAEYMVRASGYLQTLDDFNHIVLKASENGVPVYLRDVAKVQVGPEMRRGIA
                     ELNGEGEVAGGVVILRSGKNAREVIAAVKDKLETLKSSLPEGVEIVTTYDRSQLIDRA
                     IDNLSGKLLEEFIVVAVVCALFLWHVRSALVAIISLPLGLCIAFIVMHFQGLNANIMS
                     LGGIAIAVGAMVDAAIVMIENAHKRLEEWQHQHPDATLDNKTRWQVITDASVEVGPAL
                     FISLLIITLSFIPIFTLEGQEGRLFGPLAFTKTYAMAGAALLAIVVIPILMGYWIRGK
                     IPPESSNPLNRFLIRVYHPLLLKVLHWPKTTLLVAALSVLTVLWPLNKVGGEFLPQIN
                     EGDLLYMPSTLPGISAAEAASMLQKTDKLIMSVPEVARVFGKTGKAETATDSAPLEMV
                     ETTIQLKPQDQWRPGMTMDKIIEELDNTVRLPGLANLWVPPIRNRIDMLSTGIKSPIG
                     IKVSGTVLADIDTMAEQIEEVARTVPGVASALAERLEGGRYINVEINREKAARYGMTV
                     ADVQLFVTSAVGGAMVGETVEGIARYPINLRYPQSWRDSPQALRQLPILTPMKQQITL
                     ADVADVKVSTGPSMLKTENARPTSWIYIDARDRDMVSVVHDLQKAIAEKVQFKPGTSV
                     AFSGQFELLERANHKLKLMVPMTLMIIFVLLYLAFRRVGEALLIISSVPFALVGGIWL
                     LWWMGFHLSVATGTGFIALAGVAAEFGVVMLMYLRHAIEAEPSLNNPQTFSEQKLDEA
                     LYHGAVLRVRPKAMTVAVIIAGLLPILWGTGAGSEVMSRIAAPMIGGMITAPLLSLFI
                     IPAAYKLMWLHRHRVRK"
     gene            complement(9022..10245)
                     /gene="cusB"
                     /locus_tag="J1Y83_002566"
     CDS             complement(9022..10245)
                     /gene="cusB"
                     /locus_tag="J1Y83_002566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter periplasmic
                     adaptor subunit CusB"
                     /protein_id="HBA7630708.1"
                     /translation="MKKIALIIGSMIAGGIISAAGFTWFAKAELPAEKTSTAERKVLF
                     WYDPMYPNTRFDKPGKSPFMDMDLVPKYADEESSASGVRIDPTQTQNLGVKTATVTRG
                     PLTFAQSFPANVSYNEYQYAIVQARAAGFIDKVYPLTVGDKVQKGAPLLDLTIPDWVE
                     AQSEYLLLRETGGTATQTEGILERLRLAGMLEADIRRLIATQKIQTRFTLKAPIDGVI
                     TAFDLRAGMNIAKDNVVAKIQGMDPVWVTAAIPESIAWLVKDASQFTLTVPARPDKTL
                     TIRKWTLLPGVDAATRTLQLRLEVDNADEALKPGMNAWLQLNTASEPMLLIPSQALID
                     TGNEQRVITVDADGRFVPKRVAVFQASQGVTALRSGLAEGEKVVSSGLFLIDSEANIS
                     GALERMRSESATHAH"
     gene            complement(10261..10593)
                     /gene="cusF"
                     /locus_tag="J1Y83_002567"
     CDS             complement(10261..10593)
                     /gene="cusF"
                     /locus_tag="J1Y83_002567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter periplasmic
                     metallochaperone CusF"
                     /protein_id="HBA7630709.1"
                     /translation="MKKALQVAMFSLFTVIGFNAQANEHHHETMSEAQPQVISATGVV
                     KCVDLESKKITIHHDPIAAVNWPEMTMRFTITPQTKMSEIKTGDKVAFNFVQQGSLSL
                     LQDIKVSQ"
     gene            complement(10617..11999)
                     /gene="cusC"
                     /locus_tag="J1Y83_002568"
     CDS             complement(10617..11999)
                     /gene="cusC"
                     /locus_tag="J1Y83_002568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter outer
                     membrane channel CusC"
                     /protein_id="HBA7630710.1"
                     /translation="MSPCKLLPFCVALALTGCSLAPDYQRPAMPVPQQFSLSQNGLVN
                     AADNYQNAGWRTFFVDNQVKTLISEALVNNRDLRMAALKVQEARAQYRLTDADRYPQL
                     NGEGSGSWSGNLKGDSATTREFSTGLNASFDLDFFGRLKNMSEAERQNYLATEEAQRA
                     VHILLVSNVAQSYFNQQLAYAQLQIAEETLRNYQQSYAFVEKQLLTGSSNVLALEQAR
                     GVIESTRSDIAKRQGELAQANNALQLLLGSYGKLPQAQTVNSDSLQSVKLPAGLPSQI
                     LLQRPDIMEAEHALMAANANIGAARAAFFPSISLTSGISTASSDLSSLFNASSGMWNF
                     IPKIEIPIFNAGRNQANLDIAEIRQQQSVVNYEQKIQNAFKEVADALALRQSLNDQIS
                     AQQRYLASLQITLQRARALYQHGAVSYLEVLDAERSLFATRQTLLDLNYARQVNEISL
                     YTALGGGWQQ"
     gene            12156..12839
                     /gene="cusR"
                     /locus_tag="J1Y83_002569"
     CDS             12156..12839
                     /gene="cusR"
                     /locus_tag="J1Y83_002569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper response regulator transcription factor
                     CusR"
                     /protein_id="HBA7630711.1"
                     /translation="MKLLIVEDEKKTGEYLTKGLTEAGFVVDLADNGLNGYHLAMTGD
                     YDLIILDIMLPDVNGWDIVRMLRSANKGMPILLLTALGTIEHRVKGLELGADDYLVKP
                     FAFAELLARVRTLLRRGAAVIIESQFQVADLMVDLVSRKVTRSGTRITLTSKEFTLLE
                     FFLRHQGEVLPRSLIASQVWDMNFDSDTNAIDVAVKRLRGKIDNDFEPKLIQTVRGVG
                     YMLEVPDGQ"
     gene            12829..14271
                     /gene="cusS"
                     /locus_tag="J1Y83_002570"
     CDS             12829..14271
                     /gene="cusS"
                     /locus_tag="J1Y83_002570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) sensor histidine kinase CusS"
                     /protein_id="HBA7630712.1"
                     /translation="MVSKPFQRPFSLATRLTFFISLATIAAFFAFAWIMIHSVKVHFA
                     EQDINDLKEISATLERVLNHPDETQARRLMTLEDIVSGYSNVLISLADSQGKTVYHSP
                     DAPDIREFARDAIPDKDARGGEVYLLSGPTMMMPGHGHGHMEHSNWRMISLPVGPLVD
                     GKPIYTLYIALSIDFHLHYINDLMNKLVMTASVISILIVFIVLLAVHKGHAPIRSVSR
                     QIQNITSKDLDVRLDPQTVPIELEQLVLSFNHMIERIEDVFTRQSNFSADIAHEIRTP
                     ITNLITQTEIALSQSRSQKELEDVLYSNLEELTRMAKMVSDMLFLAQADNNQLIPEKK
                     MLNLADEVGKVFDFFEALAEDRGVELRFVGDECQVAGDPLMLRRALSNLLSNALRYTP
                     PGEAIVVRCQTVDHQVQVSVENPGTPIAPEHLPRLFDRFYRVDPSRQRKGEGSGIGLA
                     IVKSIVVAHKGTVAVTSDVRGTKFVIILPA"
     gene            14421..16658
                     /gene="nrfB"
                     /locus_tag="J1Y83_002571"
     CDS             14421..16658
                     /gene="nrfB"
                     /locus_tag="J1Y83_002571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage adsorption protein NrfB"
                     /protein_id="HBA7630713.1"
                     /translation="MDWLLDVFATWLYGLKVIAITLAVIMFISGLDDFFIDVVYWVRR
                     IKRKLSVYRRYPRMSYRELYKPDEKPLAIMVPAWNETGVIGNMAELAATTLDYENYHI
                     FVGTYPNDPDTQRDVDEVCARFPNVHKVVCARPGPTSKADCLNNVLDAITQFERSANF
                     AFAGFILHDAEDVISPMELRLFNYLVERKDLIQIPVYPFEREWTHFTSMTYIDEFSEL
                     HGKDVPVREALAGQVPSAGVGTCFSRRAVTALLADGDGIAFDVQSLTEDYDIGFRLKE
                     KGMTEIFVRFPVVDEAKEREQRKFLQHARTSNMICVREYFPDTFSTAVRQKSRWIIGI
                     VFQGFKTHKWTSSLTLNYFLWRDRKGAISNFVSFLAMLVMIQLLLLLAYESLWPDAWH
                     FLSIFSGSAWLMTLLWLNFGLMINRIVQRVIFVTGYYGLTQGLLSVLRLFWGNLINFM
                     ANWRALKQVLQHGDPRRVAWDKTTHDFPSVTGDTRSLRPLGQILLENQVITEEQLDTA
                     LRNRVEGLRLGGSMLMQGLISAEQLAQALAEQNGVAWESIDAWQIPSSLIAEMPASVA
                     LHYAVLPLRLDNDELIVGSEDGIDPVSLAALTRKVGRKVRYVIVLRGQIVTGLRHWYA
                     RRRGHDPRAMLYNAVQHQWLTEQQAGEIWRQYVPHQFLFAEILTTLGHINRSAINVLL
                     LRHERSSLPLGKFLVTEGVISQETLDRVLTIQRELQVSMQSLLLKAGLNTEQVAQLES
                     ENEGE"
     gene            16645..19617
                     /gene="nfrA"
                     /locus_tag="J1Y83_002572"
     CDS             16645..19617
                     /gene="nfrA"
                     /locus_tag="J1Y83_002572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriophage adsorption protein NfrA"
                     /protein_id="HBA7630714.1"
                     /translation="MKENNLNRVIGWSGLLLTSLLSTSALADNIGTSAEELGLSDYRH
                     FVIYPRLDKALKAQKNNDEATAIREFEYIHQQVPDNIPLTLYLAEAYRHFGHDDRARL
                     LLEDQLKRHPGDDRLERSLAAIPVEVKSVTTVEELLAQQKACDAAPTLRCRSEVGQNA
                     LRLAQLPVARAQLNDATFAASPEGKTLRTDLLQRAIYLKQWSQADTLYNEARQQNTLS
                     AAERRQWFDVLLAGQLDDRILALQSQGIFTDPQSYITYATALAYRGEKARLQHYLIEN
                     TPLFTTDAQEKSWLYLLSKYSGNPVQALANYTVQFADNRQYVVGATLPVLLKEGQYGA
                     AQKLLATLPANEMLEERYAVSVATRNKAEALRLARLLYQQEPANLTRLDQLTWQLMQN
                     EQSREAADLLLQRYPFQGDARVSQTLMARLASLLESHPYLATPAKVAILSKPLPLAEQ
                     RQWQSQLPGIADNCPAIVRLLGDMSPSYDATAWNRLAKCYRDTLPGVALYAWLQAEQR
                     QPDAWQHRAVAYQAYQVEDYATALAAWQKISLHDMSNEDLLAAANTAQAAGNGAVRDR
                     WLQQAEQRGLGNNALYWWLHAQRYIPGQPELALNDLTRSINIAPSANAYVARATIYRQ
                     RHNVPAAVSDLRAALELEPNNSNTQAALGYALWDSGDIAQSREMLEQAHKGLPDDPAL
                     IRQLAYVNQRLDDMPATQHYARLVIDDIDNQALITPLTPEQNQQRFNFRRLHEEVGRR
                     WTFSFDSSIGLRSGAMSTANNNVGGAAPGKSYRSYGQLEAEYRLGRNMLLEGDLLSVY
                     SRVFADTGENGVMMPVKNPMSGTGLRWKPLRDQIFFLAVEQQLPLNGQNGASDTMLRA
                     SASFFNGGKYSDEWHPNGSGWFAQNLYLDAAQYIRQDIQAWTADYRVSWHQKVANGQT
                     IEPYAHVQDNGYHDKGTQGAQLGGVGVRWNIWTGETHYDAWPHKVSLGVEYQHTFKAI
                     NQRNGERNNAFLTIGVHW"
     gene            19618..20508
                     /locus_tag="J1Y83_002573"
     CDS             19618..20508
                     /locus_tag="J1Y83_002573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4434 family protein"
                     /protein_id="HBA7630715.1"
                     /translation="MRKFIFVLLTLLLVSPFSFAMKGIIWQPQNRDSQVTDTQWQGLM
                     SQLRLQGFDTLVLQWTRYGDAFTQPEQRALLFKRAAAAQQAGLKLIVGLNADPEFFMH
                     QKQSSAALESYLNRLLAADLQQARLWSAVPGVTPDGWYISAEIDDLNWRSEAARQPLL
                     TWLNNAQRLISDVSAKPIYISSFFAGNMSPDGYRQLLEHVKATGVNVWVQDGSGVDKL
                     TAEQRERYLQASVDCQSSAPASGIVYELFVAGKGKTFTAKPKPNAEIASLLAKRSSCG
                     KDTLYFSLRYLPAAQSILEY"
BASE COUNT         5102 a         5651 c         5284 g         5013 t
ORIGIN      
        1 aaatcctgtt tcggacttct cactcctcat ttaatcaact gaagctgaac ccgttttttt
       61 ctgcatgcct caatcgtcaa aattcttcgc cgccagccag ctctgccata acttgtcaca
      121 ttgcaaaagt agccaaattg cttcagtcac aatcagttct ccatcaccaa aaaaagaaac
      181 attccagaac ttccgcatct cacccagccg ctcccacttc tatactgaat agaaaacgcc
      241 aacataagag aaacctatgc cattacccga ttttcatgtt tctgaacctt ttaccctcgg
      301 tattgaactg gaaatgcagg tggttaatcc gccgggctat gacttaagcc aggactcatc
      361 aatgctgatt gacacggtta aaaatcagat cacggccgga gaggtaaagc acgatataac
      421 cgaaagtatg ctggagctgg cgacggatgt ttgccgtgat atcaaccagg ctgccgggca
      481 attttcagcg atgcagaaag tcgtattgca ggcagcggca gaccatcatc tggaaatttg
      541 cggcggtggc acgcatccgt ttcagaaatg gcagcgtcag gaggtatgcg ataacgaacg
      601 ctatcaacga acgctggaaa actttggcta tctcatccag caggcgaccg tttttggtca
      661 gcatgtccat gttggctgcg ccagtggcga tgacgccatt tatttgctgc acggcttgtc
      721 acggtttgtg ccgcacttta tcgccctttc cgccgcgtcg ccatatatgc agggaacgga
      781 tacgcgtttt gcctcctcac gaccgaatat tttttccgcc tttcccgata atggcccaat
      841 gccgtgggtc agtaactggc aacaatttga agccctgttt cgctgcctga gttacaccac
      901 gatgatcgac agcattaaag atctgcactg ggatattcgc cccagccccc attttggtac
      961 ggtggaagtt cgggtgatgg ataccccgtt aacccttagc cacgcggtaa atatggcggg
     1021 attaattcag gccaccgccc actggttact gacggaacgc ccgtttaaac atcaggagaa
     1081 agattacctg atgtataaat tcaaccgttt ccaggcctgt cgctatgggc tggaaggtgt
     1141 cattaccgat ccgcatactg gcgatcgtcg gtcactaacg gaagccacct tgcgtttact
     1201 ggaaaaaatc gccccttctg cacataaaat tggcgcatcg agcgcaattg aagcactgca
     1261 ccgtcaggtc gtcagtggtc tgaatgaagc gcagctaatg cgtgatttcg tcgccgatgg
     1321 tggctcgcta attgggctgg tgaaaaagca ttgtgagatc tgggccggag aataagtcgg
     1381 gaattgcctt acagaagaat atccctgaca atggtgtttt aacttacatt taacaatgtt
     1441 atgaaacacc ctttagaaac cttgaccacc gcagcaggca ttttgctgat ggctttcctc
     1501 tcttgcctgc tgctgcccgc ccccgcactg gggctgacgc tggcacaaaa actggtgacc
     1561 acgttccatc tgatggatct tagccagctt tacactgtat tgttttgtct gtggttttta
     1621 gtgctgggcg ctattgagta ttttgttcta cgctttatct ggcgacgctg gttctcgctg
     1681 gcggattaaa cgtcgattat cgccgaactt cgcggcatac tttgcttatt ctccttcgcc
     1741 taaaggaaca tttatggata agcaatcact gcacgaaacg gcgaaacgcc tggctcttga
     1801 gttacccttt gtcgagcttt gctggccttt tggcccggag ttcgatgttt ttaaaattgg
     1861 cggcaagatt tttatgctgt cgtcggagct gtgcggcgtc ccctttatca atctgaagtc
     1921 cgatccacaa aaatctctgt taaatcagca aatatatccg agcattaagc ctgggtatca
     1981 catgaacaaa aaacactgga tttcggtgta tcccggcgat gaaatctccg aagcgttact
     2041 tcacgatctg atcaacgatt catggaattt agtgatcgat ggtctggcga agcgcgatca
     2101 aaaacgactg cgtcctgact caaccgtaaa tcaatagcgc atttttctcg cttaccattt
     2161 catctcgatc cttgtaatct gctggcacgc aaaattactt tcacatggag tctttatgga
     2221 tatcatttct gtcgccttaa agcgtcattc cactaaggca tttgatgcca gcaaaaaact
     2281 taccccggaa caggccgagc agatcaaaac tctactgcaa tacagcccat ccagcaccaa
     2341 ctcccagccg tggcatttta ttgttgccag cacggaagaa ggtaaagcgc gtgttgccaa
     2401 atccgctgcc gataattacg tgttcaacga gcgtaaaatg cttgatgcct cgcacgtcgt
     2461 ggtgttctgt gcaaaaaccg cgatggacga tgcctggctg aagctggttg ttgaccagga
     2521 agatgccgat ggccgctttg ccacgccgga agcgaaagcc gcgaacgata aaggtcgcaa
     2581 gttcttcgcc gatatgcacc gtaaagatct gcatgatgat gcagagtgga tggcaaaaca
     2641 ggtttatctc aacgtcggta acttcctgct gggcgtggcg gcgctgggtc tggacgcggt
     2701 gcccatcgaa ggttttgacg ccgccatcct cgatgaagaa tttggtctga aagagaaagg
     2761 ctacaccagt ctggtggttg ttccggtggg tcatcacagc gttgaagatt ttaacgctac
     2821 gctgccgaaa tctcgtctgc cgcaaaacat caccttaacc gaagtgtaat tctctcttgc
     2881 cgggcatctg cccggctatt tcccctcaga ttctcctgat ttgcataacc ctgtttcagc
     2941 cgtcatcata ggctgctgtt gtataaagga gacgttatgc aggatttaat atcccaggtt
     3001 gaagatttag cgggtattga gatcgatcac accacctcga tggtgatgat tttcggtatt
     3061 atttttctga ccgccgtcgt ggtgcatatt tttttgcact gggtggtact gcggaccttc
     3121 gaaaaacgcg ccatcgccag ttcacggctt tggttgcaaa tcattaccca gaataaactc
     3181 ttccaccgtt tagcttttac cttgcagggg attatcgtca atattcaggc ggtattctgg
     3241 ctacaaaaag gcaccgaagc ggcagatatt ctgactacct gcgcgcagtt gtggatcatg
     3301 atgtatgcgc tgctttcagt cttctcgttg ctggatgtga ttttgaatct ggcgcagaaa
     3361 ttcccggcgg catctcagtt accgctgaaa gggatttttc aggggattaa actgatcggc
     3421 gcgattctgg tcggcatttt gatgatctcg ctgctgattg gtcagtcgcc agcgattctg
     3481 atcagcggtc ttggtgcaat ggctgccgtg ctgatgttgg tatttaaaga tccgattctt
     3541 ggtctggtgg caggtattca gctttccgcg aacgatatgc tgaaactggg cgactggctg
     3601 gagatgccga aatacggcgc ggatggcgcg gtgatcgata ttgggttaac caccgtcaaa
     3661 gtacgtaact gggacaatac cattaccact attcccacct ggtctctggt ttctgactcc
     3721 tttaaaaact ggagcgggat gtcagcatct ggcgggcgac gtattaagcg cagtatcagt
     3781 attgatgtca ccagtattcg ttttcttgat gaagacgaaa tgcaacggct gaataaagcg
     3841 cacttgttaa agccttattt aaccagccgc catcaggaaa ttaatgagtg gaatcgccag
     3901 caaggttcta cggagtcggt attaaacctg cgccgaatga ccaatattgg aacctttcgt
     3961 gcctatctga acgaatatct gcggaatcat ccgcggattc gcaaagatat gaccttaatg
     4021 gtacgccaac tggctccggg cgataacggt ttaccgcttg agatctatgc gtttaccaac
     4081 accgtggtgt ggctggaata tgaaagcatt caggctgata tattcgatca catatttgcg
     4141 attgtcgaag agtttggtct gcgacttcat cagtcgccaa ccggcaatga tattcgctct
     4201 ctggcgggtg catttaagca gtaataaaaa aaaaccgctc tcatcgaatg gatgagagcg
     4261 gctttcagat tgttgacatc attttgtcgg atgcggcgtg aacgccttat ccgacctaca
     4321 gaatcgttac aaattgtagg cccgataagc ttgcgcatcg ggcaaatcag cctcacatca
     4381 gcttaattca ctttcctacg caatgtttta aacgccacaa acaggaacac aatccacacc
     4441 ggcagcagaa tcgctgacaa gcgcatatca tccatcgtgc acatcagcag caaaatcatg
     4501 ccgaggaagg cgatgcaaag atagttgccg aacggataga gcagcgcctt aaactgtgtt
     4561 tcacgcccct gacgacgcat cgctgcacga aaacgcagat gcgccaggca gatcataatc
     4621 cagttcaaca gcagcgttgc taccaccagc gccatcagca gaccaaacgc tttttgcggc
     4681 agcagatagt tgattaacac caccagcgaa gtgatcgctc cggaaagcat cagcgagtta
     4741 atcggcacgc cgcgacggct gacgcgagtc aaaaactttg gcgcattacc ctgcacagaa
     4801 aggccaaaca gcatgcggct gttagagtaa accccgctgt tatacaccga cagcgatgcc
     4861 accagaatga cgaagttcag cgtagaagcc accacgttgc tgtcgagatt atggaaaatc
     4921 atcacaaacg ggctactgtt ggatttcact tccacccacg ggtagagcgc cagtaaaacc
     4981 accagtgaac cgatataaaa cagcaggatg cgatacacca cctgattcac tgctttaggg
     5041 atgctttttt ccggatcgcg cgcttcagcc gcagtaatcc caatcagctc cagaccgccg
     5101 aaggagaaca taattaccgc cagcgacaaa atcagtccat tccagccggt ggcaaagaaa
     5161 ccgccgtagc gccagaggtt atcgatactg gctttctcac cgccgtgccc agaaaacagc
     5221 agccacaggc caaagccgat cataccgatg atcgccagca ctttaatcag cgcaaaccag
     5281 aactcggttt cgccatataa gcgcacgttc accaggttaa cggcgttgat gataataaag
     5341 aaggcggcag cccaaatcca cgttggaaca tccgggaacc agtactgcat atagatgccc
     5401 gcagcggtca gctctgccat tcccaccagc acgaacatta cccagtagtt ccagccagag
     5461 aggaagcccg caaacggtcc ccagtattta taggcaaagt gggcaaatga accggatacc
     5521 ggctcttcga ccaccatctc gccgagctgg cgcataatca ggaaagcgat gatcccggcg
     5581 acgccgtagc ccagcaatac agccggaccc gccatctgaa tcgccgggcc aatgccaaga
     5641 aacagaccag taccaattgc gccacccagc gcaatcagtt gaatatgacg gttatgtaat
     5701 ccgcgatgaa gcgtcggctc ttgattcgac gcagtatctt ccgatacggt tgacgcgttt
     5761 ttcacgcctt tcccctgtgt gtcttttttg ttgaggggca ccttttaaca tttagtgccc
     5821 atcgtcgcaa gacacaatcc acacggttaa acggggtatc ctgcttttat ttccgtaccc
     5881 gatgtcggtg cagccacatc agcttatacg ccgccgggat aataaacagc gacagcaaag
     5941 gcgcggtgat catgccgcca atcatcggcg cggcaatccg gctcatcacc tctgaccctg
     6001 ctcctgttcc ccacagaatc ggcagcagac cagcgataat caccgccacc gtcatcgctt
     6061 tcgggcgcac gcgcagtacc gcgccgtgat ataacgcctc atccagcttc tgctcgctga
     6121 atgtttgcgg attattcaac gacggctcgg cctctatggc gtgacgtaaa tacatcagca
     6181 tcaccacgcc aaattcggcg gcgaccccgg caagggcaat aaagccggtg cccgttgcca
     6241 cagaaagatg aaagcccatc caccacagca accagatgcc gcccaccagc gcaaacggta
     6301 cgctgctgat aatcagcaac gcttcgccca cccgacggaa cgccagatac aacagcacga
     6361 agatgatcat caacgtcatc ggcaccatca gtttcagctt atggttggcg cgctccagta
     6421 gctcgaactg cccggagaat gccacgctgg tgcccggttt aaactgcact ttttcagcta
     6481 tcgctttttg caaatcgtga accaccgaca ccatgtcacg atcgcgggca tcgatataaa
     6541 tccagctcgt cgggcgcgca ttctcggttt tcagcatcga cggtccggta gagactttaa
     6601 cgtcggccac gtctgccagg gtgatttgct gcttcatcgg cgtgaggatc ggcaactggc
     6661 gcagtgcctg cgggctatcg cgccagcttt gcggataacg cagattaatt ggataacgag
     6721 caatcccttc caccgtttcg ccaaccatcg ccccgcccac cgcagaagtc acaaacaact
     6781 gcacatccgc caccgtcata ccgtaacgcg cggctttttc acggttaatc tcaacgttga
     6841 tatagcgccc accttccaga cgctcagcaa gcgcagaagc cacgcctggc accgttcgcg
     6901 ccacttcttc aatttgctcc gccatcgtgt cgatatccgc cagcacagtg ccggaaactt
     6961 taatgccgat agggctttta atgccagtcg agagcatatc gatacggtta cgaattggcg
     7021 gcacccacag attcgccagc cccggcagac gcacggtgtt atccagttcc tcaatgattt
     7081 tgtccatcgt catgccaggc cgccactgat cctgcggctt aagctggata gtcgtttcta
     7141 ccatctccag tggagctgaa tcggtggcgg tttccgcttt cccggttttg ccaaataccc
     7201 gcgccacttc cggtacgctc ataatcagct tgtcggtttt ttgcagcatg ctcgccgcct
     7261 ccgctgcgga aatccccggc agtgtcgatg gcatatacaa caagtcgcct tcattgatct
     7321 gcggtaaaaa ttccccgcca actttattga gcggccagag aaccgtcagc accgaaagcg
     7381 ctgccaccaa cagcgtggtt ttcggccagt gcagtacttt cagcaacagc ggatgataaa
     7441 cacgaatcaa aaagcgattg agcgggttac tgctttccgg cggaattttg ccacggatcc
     7501 agtagcccat caggatcggg atcactacga tcgccagtag cgccgcgccc gccatcgcat
     7561 aggttttggt gaacgccagc gggccaaaca gacgaccttc ctgcccttcc agggtgaaga
     7621 tcgggataaa cgacagcgtg ataatcagca gactgataaa cagcgccgga cccacttcaa
     7681 cagatgcatc ggtgatcacc tgccagcgcg ttttattatc caacgtggcg tcaggatgct
     7741 ggtgctgcca ctcttccagc cgtttatgcg cattctcgat catgacgata gcggcatcga
     7801 ccatcgcccc gacggcaatc gcaatgccac ccaacgacat aatattggcg ttcagtccct
     7861 gaaagtgcat gacaataaaa gcaatacaca accccagcgg caacgaaata atcgccacca
     7921 gcgccgagcg cacatgccag agaaacagcg cacagactac cgccaccaca ataaactctt
     7981 ccagcaactt gccgctgagg ttgtcaatgg cgcggtcaat gagctggctg cgatcgtatg
     8041 ttgtaactat ctccacgcct tccggcagac tgcttttcag cgtttccagt ttgtccttca
     8101 cggcggcgat cacttcacgg gcgtttttgc cggaacgcag gatcaccacc ccgcccgcca
     8161 cttcgccttc gccgttcagt tcggcaatgc cccggcgcat ctccgggcca acctggacct
     8221 tcgcaacatc acgcagataa acgggcacgc cattttcact ggcttttaaa acgatgtgat
     8281 taaagtcgtc gagcgtttgc agatagccgc tggcgcgcac catatattcc gcttccgcca
     8341 gttcgatcga cgaaccgccc gcttcctggt ttgaagcatc cagcgcgctt tttacttcgg
     8401 cgagactgat gccatactgc gccaggcgct gaggatcgat aaccacctga tactctttca
     8461 ccacaccgcc caccgacgcc acttccgcaa cgtcagggat ggtttttagc tcatatttga
     8521 gaaaccagtc ctgcaatgag cgtaaatcgg ccagatcgtg cttaccgctg cgatccacca
     8581 gtgcatattc atagatccag ccaacacccg tggcatctgg ccccagctcg gcgctgactc
     8641 ccgcaggcag cttaccctgt acctggttga ggtactccag cacccgcgag cgcgcccagt
     8701 acggatcggt gccatcttcg aaaatgacat acacataaga gtcgccaaac tgcgagaaac
     8761 cgcgcacagt cttcgcgcca ggcaccgaca acatggtggt ggttagcgga taagtcacct
     8821 gattttcaac gatttgcggt gcctgaccgg gatagctggt tttaataatc acctgtacat
     8881 cagagagatc cggcagtgca tccactggcg tgttaatgat ggtccaggtg ccccagatac
     8941 tcagaaacaa cgcgcccatc agcaccagaa aacggttcgc caccgagcga cgaataatcc
     9001 attcaatcat tggctattcc ctcaatgcgc atgggtagca ctttcagagc gcatccgctc
     9061 cagtgcgcca gaaatattgg cttcagaatc aatcaggaac aggccgctgg aaaccacctt
     9121 ttcaccttcc gccagaccag agcgtaacgc ggtgacgcct tgcgatgcct ggaaaacggc
     9181 aacgcgtttc ggtacaaagc gcccgtcagc atcaacggta atcacccgct gttcgttgcc
     9241 ggtatcaatc agcgcctgtg acggaatgag cagcatcggt tcgctggcgg tattgagttg
     9301 caaccaggcg ttcatgcccg gttttagcgc ctcgtcggcg ttgtcgactt ccagacgcag
     9361 ttgcagcgtg cgggtcgcag catccacgcc aggtagcagc gtccatttgc ggatggtgag
     9421 tgttttatca ggtcgcgccg gaacggtgag cgtaaactgc gaggcatctt tcaccagcca
     9481 ggcgatggac tccgggatcg cagcagtgac ccacaccggg tccataccct gaattttcgc
     9541 gaccacgtta tctttggcga tattcattcc cgcgcgcaga tcaaacgcgg tgatcacgcc
     9601 atcaatgggc gctttgagcg taaagcgagt ctggattttt tgcgtggcga tcaggcggcg
     9661 aatatcagcc tccagcattc ccgccagtcg cagtcgctca agaatgcctt cagtctgggt
     9721 cgccgtaccg ccggtttcgc gcagcagtaa atactcactc tgcgcttcca cccagtcggg
     9781 aatggtcagg tcgagaagcg gcgcgccctt ttgcacttta tcgcccacgg taagcggata
     9841 caccttgtcg ataaacccgg cggcgcgggc ctgcacaatg gcatactgat actcgttgta
     9901 actgacattt gccgggaaac tctgggcaaa agtcagcggc ccacgcgtga cggtcgccgt
     9961 tttcacaccc agattctgcg tctgagtcgg gtcaatgcgc acaccagatg cagaactctc
    10021 ttcatcggca tatttcggca ccagatccat atccataaac ggcgatttcc ctggtttatc
    10081 gaaccgcgta ttgggataca tcgggtcgta ccagaataag actttacgtt ctgcggtcga
    10141 cgttttttct gcgggcagtt cagcctttgc aaaccaggta aaacctgccg cagaaataat
    10201 accgcccgcg atcatgctgc cgataataag cgcgattttt ttcatctcat taaacctggg
    10261 ttactggctg actttaatat cctgtaataa agaaaggctg ccctgctgga caaaattaaa
    10321 cgccactttg tcgccggttt taatttcgct cattttcgtc tgcggggtga tggtaaagcg
    10381 catggtcatc tccggccagt tcacggcagc aatcggatcg tgatggatgg tgattttttt
    10441 gctttccaga tcaacacatt ttaccacacc agtggcgcta ataacctgtg gttgtgcttc
    10501 gctcatggtt tcatgatgat gttcgttagc ctgggcatta aagccaataa cggtaaacag
    10561 actaaacatt gcgacttgca gtgctttttt catttattct ctcctggagt taaaaattat
    10621 tgctgccaac cgccgcctaa ggcggtatat aaagaaattt cgttaacctg acgggcataa
    10681 ttcaggtcaa gtaaagtttg tcgggttgca aataaagaac gctcggcatc cagcacttcc
    10741 agataactta ccgcgccgtg ctgatataat gcccgcgccc gttgcaaagt aatttgcagc
    10801 gacgcaagat aacgctgctg ggcgctgatt tgatcgttca ggctttgacg taatgccagc
    10861 gcatctgcca cttctttaaa ggcgttctgg attttctgtt cataattcac caccgactgc
    10921 tgctggcgaa tttcggcgat atccagattg gcctggttgc gtccggcatt aaaaatgggg
    10981 atctcaattt taggaataaa attccacatc ccgctgctgg cgttaaataa tgacgacaga
    11041 tcgctgctgg cggttgatat tccgctggtc aggctgatag acgggaaaaa tgccgcacgc
    11101 gcagcaccaa tattggcatt agccgccatt aacgcgtgtt cagcttccat aatatcaggg
    11161 cgctgcaata agatttgcga cggcaagccc gccggtaatt taacgctttg caggctgtcg
    11221 ctgtttactg tctgcgcttg cggcagcttg ccgtagcttc ccaataacag ttgcaatgca
    11281 ttatttgcct gcgccagttc cccctgacgt ttagcgatgt cgctgcgggt actttctatc
    11341 accccgcgag cctgttccag cgccagaaca ttgctgctac cggtcaacag ttgtttttcg
    11401 acaaacgcat atgactgctg ataattacgc agcgtttctt cggctatttg caattgcgca
    11461 tacgccagct gctgattgaa atagctttgt gcgacattag aaaccagcaa aatatgcacc
    11521 gcgcgctgag cttcctcagt ggctaaataa ttttggcgct cggcttcgct catgttcttt
    11581 aagcgcccga aaaaatcgag atcaaagctg gcgttaaggc cagtcgagaa ctcccgcgtc
    11641 gtggctgaat cgcctttaag attgccgctc cagctgccgc tgccctcgcc attgagctgt
    11701 gggtagcggt cggcatcggt cagacgatat tgcgcccgcg cttcctgcac tttcagcgcc
    11761 gccatgcgca aatcccggtt attcaccagc gcctcgctaa tcagcgtctt cacctgatta
    11821 tcaacaaaaa aggtgcgcca gcccgcgttc tggtagttat ctgctgcgtt aaccaggccg
    11881 ttctggctga gtgagaactg ctgcggcacg ggcattgccg gacgctgata atccggtgcc
    11941 agtgaacaac cggttagcgc aagggccaca caaaatggca gaagtttaca aggagacata
    12001 ggctcataat ttctggtgat tttatgctgc caactttact cgccaggctc tgattttccg
    12061 gtgacaggaa aatgacaaaa ttgtcatttt gccaataagc gattgccatc tgatcccgct
    12121 actctagaat tgcccgggca acatgcggag gaaatatgaa actgttgatt gtcgaagatg
    12181 aaaagaaaac cggagaatac ttgaccaaag ggttaaccga agccggtttt gtggtcgatt
    12241 tggccgacaa cgggctgaat ggctaccatc tggcgatgac cggtgattat gatctgataa
    12301 tcctcgatat tatgctgccg gacgtgaacg gctgggatat cgtgcgcatg ttgcgttccg
    12361 ccaataaggg gatgccgatt ctgttgctta ccgcgcttgg caccattgaa catcgcgtca
    12421 aggggctgga gttgggggcg gatgactatc tggtgaagcc gttcgctttt gctgaactgc
    12481 tggcgcgggt gcgcactcta ctgcggcgcg gggcggcggt gattatcgaa agtcagtttc
    12541 aggttgccga tctgatggtc gatctcgtca gccgcaaagt cacccgcagc ggcacgcgca
    12601 tcactctgac cagtaaagaa tttactctgc tggagttttt cctccgccat cagggcgaag
    12661 tgctgccccg ctcgcttatc gcctcgcagg tatgggacat gaattttgac agcgatacca
    12721 acgccattga tgtggcggtg aagcggctgc gcggcaaaat cgacaacgac tttgagccaa
    12781 agttgattca gaccgtgcgc ggcgtgggtt acatgctaga ggtgccggat ggtcagtaag
    12841 ccatttcagc gcccgttttc gctggcaacc cgcctgacct tttttatcag cctcgccacc
    12901 atcgcggcgt ttttcgcctt tgcatggatc atgatccact cggtaaaagt gcattttgcc
    12961 gagcaggata ttaatgattt aaaggagatt agcgccaccc ttgaacgggt acttaatcac
    13021 cctgacgaaa cgcaagcccg acgcttaatg acgctggaag atattgtcag tggttattcc
    13081 aatgtgttga tctccctggc agatagccag ggtaaaacgg tgtatcactc ccccgacgcg
    13141 cccgatatcc gcgagtttgc acgtgatgcc atacccgata aagacgctcg gggcggcgag
    13201 gtgtatctcc tttccggccc gacaatgatg atgccaggcc acggtcacgg acatatggaa
    13261 cacagcaact ggcggatgat tagcttgccg gttggcccac tggtggacgg caaaccgatt
    13321 tatacgctct acattgcgct ttcgatcgat tttcatcttc attacataaa tgatttgatg
    13381 aataaacttg taatgaccgc atcggtaatc agcatcctga tcgtctttat cgtgctgttg
    13441 gcggtacata aaggtcacgc gccgatccgc agcgtcagcc gtcaaatcca gaacattacc
    13501 tcgaaagatc tcgacgttcg cctcgatcca cagaccgtgc ccattgaact cgaacaactg
    13561 gtgctgtcgt tcaaccatat gatcgagcgt attgaggatg tctttacccg ccagtccaat
    13621 ttctcagcgg atatcgccca cgaaattcgc acaccaatta cgaatctcat aacgcaaacg
    13681 gaaatcgccc tcagccagtc gcgcagccag aaggagctgg aagatgtgct ctactctaat
    13741 ctcgaagagc tgacgcgaat ggcgaaaatg gtcagcgata tgttgtttct cgcccaggcc
    13801 gataacaacc agttaatccc cgaaaagaaa atgctcaacc tggcggatga agtcggcaaa
    13861 gtgttcgatt ttttcgaggc gttagcggaa gatcgcggcg tggagttgcg gtttgttggt
    13921 gacgagtgtc aggttgcggg cgatccgctg atgttgcgtc gggcgttaag caacctgctt
    13981 tcgaacgccc tgcgttatac gccacccgga gaggcaattg ttgtgcgctg ccagacggtc
    14041 gatcaccagg tgcaagtttc cgtcgaaaac cccggtacgc ccattgcgcc cgaacactta
    14101 ccgcgattgt ttgaccgttt ctaccgcgtt gatccttccc gccagcgaaa aggcgaaggt
    14161 agcggcattg gcctggcgat agtgaaatcg attgttgtcg ctcataaagg cacagttgca
    14221 gtaacgtcag atgtgcgggg gacaaagttt gttatcatat tacccgctta atccttcagc
    14281 aaacggcaac ttttataacc agtgtaaaaa taacatgccg caatagtaat acctattaat
    14341 gataaaaagc tgtcacaatt cataaaaaac cttaatatac gccaccctaa acataaccag
    14401 cgttaatgta aggtttttgt gtggactggc ttcttgatgt ttttgctacc tggctctacg
    14461 gcttaaaagt aatcgcgata acgttagcgg tcattatgtt catcagcggg ctggacgatt
    14521 tttttattga tgtcgtctac tgggtacgcc gcattaaacg caagttgagt gtttatcgcc
    14581 gctacccgcg aatgagttac cgcgaactgt ataaaccaga tgaaaaaccg ttagcgatta
    14641 tggttccggc gtggaatgaa acgggcgtca tcggcaatat ggccgagctg gcggcgacca
    14701 cgctcgacta cgaaaactat catatctttg ttggcaccta ccccaacgac cccgatactc
    14761 agcgtgatgt tgacgaagtg tgcgctcgct tcccgaacgt gcataaggta gtctgcgcgc
    14821 gtcctggccc caccagcaaa gccgactgtc tgaacaacgt gctggatgcc atcacccaat
    14881 ttgaacgtag cgccaatttc gcttttgctg gttttattct gcatgacgcc gaagatgtga
    14941 tttcaccgat ggaattgcgt ctgttcaact atctggtcga gcgtaaagat ctgattcaga
    15001 tcccagtgta tccgttcgaa cgcgaatgga cgcacttcac cagcatgacc tacattgatg
    15061 agttttcaga actgcatggc aaagatgttc cggtgcgtga agccctcgcc ggacaggtgc
    15121 ccagcgcagg cgtcggcacc tgtttcagcc gccgcgccgt gaccgcactg ttagctgacg
    15181 gtgacggtat tgctttcgac gtgcagagtc tgactgaaga ttacgacatt ggcttccgcc
    15241 tgaaagagaa aggtatgacg gaaatttttg tccgttttcc ggtggtggac gaagccaaag
    15301 aacgcgagca gcgtaaattt ttacagcatg cacggacgtc aaatatgatc tgcgtgcgcg
    15361 aatatttccc cgataccttt tcgactgcgg ttcggcaaaa atctcgctgg atcatcggca
    15421 ttgttttcca aggctttaaa acccacaaat ggacctccag cctgacgctg aactactttc
    15481 tctggcgcga ccgcaaaggg gcaatcagta actttgtcag cttcctcgcg atgctggtga
    15541 tgatacagct tttgctgttg ctggcgtatg aaagtttgtg gcccgatgcc tggcatttcc
    15601 tttctatttt cagcggcagc gcatggttaa tgactctgct gtggctaaac tttggcttga
    15661 tgattaaccg catcgtgcag cgggtgattt tcgttactgg ctactacggc ctgacgcagg
    15721 ggctgctatc tgtcctgcgt cttttctggg gcaacctgat taacttcatg gccaactggc
    15781 gcgcgttaaa acaggtactt caacacggcg atccacgtcg cgtcgcgtgg gataaaacaa
    15841 cgcatgactt ccccagcgtg actggcgata cccgctcgtt gcgtccgtta ggtcaaatcc
    15901 tgctggaaaa tcaggtcatc actgaagaac aactcgatac agcactgcgt aatcgcgtcg
    15961 aaggtctacg cctgggcggt tcaatgctga tgcaggggct gattagcgcc gagcaactgg
    16021 cacaggcgct ggcagagcaa aacggtgtgg cgtgggaatc catcgatgcc tggcagatcc
    16081 cttcctcgct gattgccgaa atgccggcct ccgtggcgct gcattatgcg gtactgccgc
    16141 tgcgtctgga caatgatgag ttaattgtcg gcagtgaaga tggtattgac ccggtttcgc
    16201 tggcggccct gacgcgtaaa gtcggacgca aagtgcgtta cgtcattgtt ctgcggggac
    16261 aaattgtcac cggattacgc cactggtatg cacgccgacg cggtcacgat ccgcgggcaa
    16321 tgttgtacaa tgcagttcag catcagtggc tcacggaaca gcaggccggt gaaatctggc
    16381 ggcaatatgt gccgcatcag ttcctgttcg cagaaatact gaccacgctc ggtcatatta
    16441 atcgttcagc aattaacgtg ttgttattgc gccatgaacg cagttctctg ccgctcggca
    16501 agtttttggt caccgaaggc gttatcagcc aggaaacgtt ggatcgcgtc ctgacaattc
    16561 aacgcgaatt acaagtttcg atgcaatcac tattactcaa agcaggttta aacactgaac
    16621 aggttgcaca actggagtcc gaaaatgaag gagaataacc ttaatcgcgt catcggatgg
    16681 tctggtttac tgctgacatc tttattgagt accagcgcac tcgcagacaa tatcggcacc
    16741 agcgcagaag agctggggct gagcgattat cgccattttg ttatttatcc ccgtctcgat
    16801 aaggcgctga aggcacagaa aaataacgac gaagcaaccg ccatccgcga atttgaatat
    16861 atacaccagc aggtgccgga taatattccg ctgactttat accttgcgga agcctatcgc
    16921 cattttggtc atgatgaccg ggcgcggctg ttgcttgagg atcaactgaa acgtcaccct
    16981 ggagatgatc ggcttgagcg cagtctggcg gctattcctg ttgaagtgaa aagcgttacg
    17041 actgttgaag aactgcttgc ccaacaaaaa gcgtgcgatg ctgcgccgac cctgcgttgt
    17101 cgcagtgaag tcgggcagaa tgccctgcgg ctggcacaat tacctgtcgc cagagcgcaa
    17161 ctgaacgatg cgacgtttgc tgcatcgccg gaaggaaaaa cgctgcgaac cgatctgctg
    17221 caacgcgcaa tctacctgaa acaatggtcc caggcagata cgctatacaa tgaagcacgc
    17281 cagcagaaca cattaagcgc ggcagaacgc cgtcagtggt ttgacgtgct tcttgccggg
    17341 cagctggacg atcggatcct ggcgctgcaa tcacagggga tcttcaccga tcctcagtca
    17401 tatattactt acgcgaccgc gctggcttat cgtggcgaaa aagcacgcct ccagcattat
    17461 ctcattgaaa atacgcctct atttaccacg gacgcacaag agaaaagttg gctctatctg
    17521 ttatctaaat acagcggcaa ccccgttcag gcgttggcga attatacggt acagtttgcc
    17581 gacaaccgcc agtatgttgt tggcgcgacg ctaccggtgc tgttaaaaga aggtcagtac
    17641 ggcgcagcgc aaaaactgct cgccaccctc cccgccaatg aaatgcttga ggagcgttat
    17701 gccgttagcg tagcgacccg taacaaggct gaagctctgc gtctggcacg attgctgtat
    17761 cagcaagaac cggcaaatct tacccgcctg gatcaactga cctggcaact gatgcagaac
    17821 gaacagtcac gcgaagctgc cgatttattg ctgcaacgct atcctttcca gggcgatgcg
    17881 cgtgtcagcc agacgttaat ggcgcgactg gcgtctctgc tggaaagtca tccttacctg
    17941 gcaacgccgg cgaaggtggc gattttatcg aaacccttac cgctggcgga gcaacgtcag
    18001 tggcaaagtc agttgccggg tattgcagat aattgcccgg caatagttcg cttgctgggc
    18061 gatatgtcgc cttcctacga tgccaccgcc tggaaccgtc tggcaaagtg ttatcgggac
    18121 acgctacccg gtgtggcgtt gtatgcatgg cttcaggccg aacaacgaca accggacgcc
    18181 tggcaacatc gtgcggtagc ctatcaggcg tatcaggttg aggactacgc caccgcactg
    18241 gcggcctggc agaaaatcag tcttcacgac atgagcaatg aggatctgct tgctgctgcc
    18301 aataccgccc aggcggcagg aaatggtgcg gttcgcgatc gctggcttca gcaggcagaa
    18361 caacgtgggc tgggaaacaa tgccctctac tggtggctgc atgcgcaacg ttacattcct
    18421 ggtcagccgg aactcgcact gaacgatctc acgcgctcaa tcaatattgc gccttctgcc
    18481 aacgcttacg ttgcgcgggc gacaatttat cgccaacgtc ataatgtccc ggcggcggtg
    18541 agtgatttgc gcgccgcgct ggaactggaa ccgaataata gcaacaccca ggcagcgctc
    18601 ggttacgcct tgtgggatag cggtgatatc gcacagtcgc gggaaatgct cgaacaggcg
    18661 cataaagggc taccggacga tccggcactt atccgacaac tggcctacgt gaaccagcgt
    18721 ctggatgaca tgcctgcgac gcagcactac gcccgactgg tgattgatga cattgataat
    18781 caggcgctga taaccccact gaccccagag caaaatcagc aacgcttcaa tttccgccgt
    18841 ctgcatgagg aggtcggtcg ccgctggacg ttcagtttcg attcttctat cggcttgcgt
    18901 tccggcgcaa tgagtaccgc taacaataat gtcggcggcg cagcgccagg gaaaagctat
    18961 cgtagctacg ggcaactgga agccgagtac cgcctcggac gcaatatgct gctggaaggc
    19021 gacctgctct cagtttatag ccgcgtcttt gccgataccg gtgaaaacgg ggtgatgatg
    19081 ccggtgaaaa atccgatgtc cggcaccggt ctgcgctgga agccgctgcg cgatcagatc
    19141 tttttcctcg ccgtcgaaca gcagttgccg ctgaacgggc aaaatggcgc atccgatacc
    19201 atgctgcgcg ccagcgcctc attctttaat ggtggcaaat acagcgacga atggcacccg
    19261 aacggttcag gctggtttgc ccaaaacctg tacctcgatg cggcgcaata tatccgccag
    19321 gatattcagg cgtggacggc agattatcgc gtcagctggc atcagaaggt ggcgaacggg
    19381 cagactattg agccttatgc tcacgttcag gacaacggct atcatgataa aggcactcag
    19441 ggcgcgcagc ttggcggtgt cggggtccgc tggaatatct ggaccggcga gacgcactac
    19501 gacgcctggc cgcacaaagt cagtctcggc gtcgaatatc aacatacctt taaggcgatt
    19561 aatcaacgta acggagagcg caacaacgcg tttctcacca ttggagtgca ctggtaaatg
    19621 cgtaagttca ttttcgtatt gttgacactg cttttggtca gccctttttc ctttgcgatg
    19681 aaaggtatta tctggcaacc acaaaaccgt gatagtcagg ttaccgatac ccagtggcag
    19741 gggctgatga gtcagttacg tttgcaaggc ttcgataccc tggttttgca atggacccgt
    19801 tacggcgatg cattcaccca gccagaacag cgcgcgttat tgtttaagcg tgcagcagct
    19861 gcgcaacagg ctggcctgaa gctgattgtc gggctgaacg ccgatccgga attttttatg
    19921 caccagaaac agtcgtccgc agcgctggaa agctatctta atcgcctgct ggctgcggat
    19981 ctccagcaag ccagattatg gagtgccgtg cctggcgtaa cgccggatgg ctggtacatc
    20041 agcgcggaaa ttgacgatct gaactggcgc agcgaagccg cccgtcagcc tttgctaaca
    20101 tggttaaaca acgcgcagcg gttgattagc gatgtttcag caaaaccgat ttatatcagt
    20161 agttttttcg ccggaaacat gtcgccagat ggctatcgcc aactgctgga acacgttaaa
    20221 gcaaccggtg ttaatgtctg ggtacaggat ggcagcggcg tggataaact gaccgctgaa
    20281 cagcgtgaac gttatctaca ggccagcgtc gattgccaaa gttccgcccc agcaagcggc
    20341 attgtttatg aactgtttgt cgctggtaaa ggtaaaacgt ttacagcgaa accgaaaccg
    20401 aacgcagaaa ttgcctcgct gttagcgaaa cgttcctctt gcggtaaaga cacgctctat
    20461 ttctctctgc gctatttgcc cgccgcgcag agtatccttg agtattaatg attaaaggga
    20521 gcaacttagc ttgctccttt ccttttcact ttcctaatca aaatcctggc aagatgaatt
    20581 gtttagcaga atttctgttt tcattttatt cgtctgacgt gttccaatta tttagcaaaa
    20641 ccgcgacaac aaagtgatgg tgtaatttgt aaaagggaac aaataaatgg cgaggaaatg
    20701 gctcgccctg caggattcga acctgtagcc cacgacttag aaactccagg aacgacattt
    20761 taaatcaaca aattaccgcg ccatctctgc gttcacacgt tccaccacct caaaatatgt
    20821 aaaaccttgc aagccattgc gaggccttat gtgtctcagt tttgttcctc ttttttatac
    20881 taaaaaacac agcaattgag gataaacctc atgctattat cgctaatatg catctaaagg
    20941 catggcactt gaatagataa aaactccaca aggaaaaaaa taattagccc ataaaaccac
    21001 aatgcaaaac acaaacagac aattaaacta aaaaactgac agtgcattgt
//
LOCUS       DADSYM010000080        21035 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2415
            SAMN08039579-rid11723173.denovo.080, whole genome shotgun sequence.
ACCESSION   DADSYM010000080 DADSYM010000000
VERSION     DADSYM010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039579
            Sequence Read Archive: SRR6362533
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 21035)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21035)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980162.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 03:39:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,484
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 4,347
            CDSs (with protein)               :: 4,347
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 43 of 85
            Pseudo Genes (incomplete)         :: 37 of 85
            Pseudo Genes (internal stop)      :: 19 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21035
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039579-rid11723173.denovo.080"
                     /strain="LGE2415"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8302 N 95.9628 W"
                     /collection_date="2015-08-24"
     gene            <1..545
                     /locus_tag="J1Y83_002574"
     CDS             <1..545
                     /locus_tag="J1Y83_002574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003843858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HBA7630716.1"
                     /translation="KAVEEVIDSPLSEVANLIKVLQSCQGKVVFIGVGKSGIIARKLA
                     ATFASTGTPSFFVHGTEAVHGDLGMVAKDDVVILISNSGETAEILATLPSLKKMGNYL
                     ISFTRSHHSSLAISCDLSVEIPVKSEADNLGLAPSCSSTVVLVVGDAVALALSELKKF
                     TRADFGLYHPGGALGIKANS"
     gene            617..1564
                     /locus_tag="J1Y83_002575"
     CDS             617..1564
                     /locus_tag="J1Y83_002575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001361319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HBA7630717.1"
                     /translation="MKNVLVTVPSFSARCVSASKLLRENNFNLIIKNNVEHLLKSEST
                     ALRESICAVIAGKDGYQADTLSLLPGVRIISRFGTGIDNIDLRAAQQSGIVVNNAVGI
                     NSNAVAEFIIGLIFASMRNIPGSYHAMQNGYWGESHGCELQGKRIGLVGYGNIGKTLA
                     KRLSGFDVELLAFDKQPDYQFADKAGVQFVSIEDIFMQSHVIIVLLPFSSELENFISH
                     KYLSMMRNGALIINAARGKLLDEGALLQVIEERNVFAALDVFSSEPLAQFSPLLHAKN
                     IITTPHIAAATVESYQQTGIHVAQSIIDYFAGREIKNVL"
     gene            complement(1644..1720)
                     /locus_tag="J1Y83_002576"
     tRNA            complement(1644..1720)
                     /locus_tag="J1Y83_002576"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1684..1686),aa:Met,seq:cat)
     gene            1929..3026
                     /gene="mltA"
                     /locus_tag="J1Y83_002577"
     CDS             1929..3026
                     /gene="mltA"
                     /locus_tag="J1Y83_002577"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase A"
                     /protein_id="HBA7630718.1"
                     /translation="MKGRWVKYLLMGTVVAMLAACSSKPTDRGQQYKDGKFTQPFSLV
                     NQPDAVGAPINAGDFAEQINHIRNSSPRLYGNQSNVYNAVQEWLRAGGDTRNMRQFGI
                     DAWQMEGVDNYGNVQFTGYYTPVIQARHTRQGEFQYPIYRMPPKRGRLPSRAEIYAGA
                     LSDKYILAYSNSLMDNFIMDVQGSGYIDFGDGSPLNFFSYAGKNGHAYRSIGKVLIDR
                     GEVKKEDMSMQAIRHWGETHSEAEVRELLEQNPSFVFFKPQSFAPVKGASAVPLVGRA
                     SVASDRSIIPPGTTLLAEVPLLDNNGKFNGQYELRLMVALDVGGAIKGQHFDIYQGIG
                     PEAGHRAGWYNHYGRVWVLKTAPGAGNVFSG"
     gene            3103..3909
                     /gene="tcdA"
                     /locus_tag="J1Y83_002578"
     CDS             3103..3909
                     /gene="tcdA"
                     /locus_tag="J1Y83_002578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA cyclic N6-threonylcarbamoyladenosine(37)
                     synthase TcdA"
                     /protein_id="HBA7630719.1"
                     /translation="MSVVISDAWRQRFGGTARLYGEKALQLFADAHICVVGIGGVGSW
                     AAEALARTGIGAITLIDMDDVCVTNTNRQIHALRDNVGLAKAEVMAERIRQINPECRV
                     MVVDDFVTPDNVAQYMNAGYSYVIDAIDSVRPKAALIAYCRRNKIPLVTTGGAGGQID
                     PTQIQVTDLAKTIQDPLAAKLRERLKSDFGVVKNSKGKLGVDCVFSTEALVYPQSDGT
                     VCAMKATAEGPKRMDCASGFGAATMVTATFGFVAVSHALKKMMAKAARQG"
     gene            complement(3960..4403)
                     /gene="csdE"
                     /locus_tag="J1Y83_002579"
     CDS             complement(3960..4403)
                     /gene="csdE"
                     /locus_tag="J1Y83_002579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase sulfur acceptor subunit
                     CsdE"
                     /protein_id="HBA7630720.1"
                     /translation="MTNPQFAGHPFGTTVTAETLRNTFAPLTQWEDKYRQLIMLGKQL
                     PALPEELKAQAKEIAGCENRVWLGYTVAENGKMHFFGDSEGRIVRGLLAVLLTAVEGK
                     TAAELQAQSPLALFDELGLRAQLSASRSQGLNALSEAIIAAAKQV"
     gene            complement(4403..5608)
                     /gene="csdA"
                     /locus_tag="J1Y83_002580"
     CDS             complement(4403..5608)
                     /gene="csdA"
                     /locus_tag="J1Y83_002580"
                     /EC_number="3.13.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase CsdA"
                     /protein_id="HBA7630721.1"
                     /translation="MNVFNPAQFRAQFPALQDAGVYLDSAATALKPEAVVEATRQFYS
                     LSAGNVHRSQFAEAQRLTARYEAAREKVAQLLNASDDKNIVWTRGTTESINMVAQCYA
                     RPRLQPGDEIIVSVAEHHANLVPWLMVAQQTGAKVVKLPLNAQRLPDVDLLPELITPR
                     SRILALGQMSNVTGGCPDLARAITFAHSAGMVVMVDGAQGAVHFPADVQQLDIDFYAF
                     SGHKLYGPTGIGVLYGKPELLEAMSPWLGGGKMVHEVSFEGFTTQSAPWKLEAGTPNV
                     AGVIGLSAALEWLTDYDINQAESWSRSLATLAEEALAKRPGFRSFRCQDSSLLAFDFA
                     GVHHSDMVTLLAEYGIALRAGQHCAQPLLAELGVTGTLRASFAPYNTKSDVDALVNAV
                     DRALELLVD"
     gene            5800..6027
                     /locus_tag="J1Y83_002581"
     CDS             5800..6027
                     /locus_tag="J1Y83_002581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgdI/YgdR family lipoprotein"
                     /protein_id="HBA7630722.1"
                     /translation="MKKTAAIISACMLTFALSACSGSNYVMHTNDGRTIVSDGKPQTD
                     NDTGMISYKDANGNKQQINRTDVKEMVELDQ"
     gene            6378..7295
                     /locus_tag="J1Y83_002582"
     CDS             6378..7295
                     /locus_tag="J1Y83_002582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GcvA"
                     /protein_id="HBA7630723.1"
                     /translation="MSKRLPPLNALRVFDAAARHLSFTRAAEELFVTQAAVSHQIKSL
                     EDFLGLKLFRRRNRSLLLTEEGQSYFLDIKEIFSQLTEATRKLQARSAKGALTVSLLP
                     SFAIHWLVPRLSSFNSAYPGIDVRIQAVDRQEDKLADDVDVAIFYGRGNWPGLRVEKL
                     YAEYLLPVCSPLLLTGEKPLKTPEDLAKHTLLHDASRRDWQTYTRQLGLNHINVQQGP
                     IFSHSAMVLQAAIHGQGVALANNVMAQSEIEAGRLVCPFNDVLVSKNAFYLVCHDSQA
                     ELGKIAAFRQWILAKAAAEQEKFRFRYEQ"
     gene            7314..7709
                     /locus_tag="J1Y83_002583"
     CDS             7314..7709
                     /locus_tag="J1Y83_002583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF423 domain-containing protein"
                     /protein_id="HBA7630724.1"
                     /translation="MTSRFMLIFAAISGFIFVALGAFGAHVLSKTMGAVEMGWIQTGL
                     EYQAFHTLAILGLAVAMQRRISIWFYWSSVFLALGTVLFSGSLYCLALSHLRLWAFVT
                     PVGGVSFLAGWALMLVGAIRLKRKGVSHE"
     gene            7702..8802
                     /gene="rlmM"
                     /locus_tag="J1Y83_002584"
     CDS             7702..8802
                     /gene="rlmM"
                     /locus_tag="J1Y83_002584"
                     /EC_number="2.1.1.186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (cytidine(2498)-2'-O)-methyltransferase
                     RlmM"
                     /protein_id="HBA7630725.1"
                     /translation="MNKVVLLCRPGFEKECAAEITDKAGQREIFGFARVKENAGYVIY
                     ECYQPDDGDKLIRELPFSSLIFARQWFVVGELLQHLPPEDRITPIVGMLQGVVEKGGE
                     LRVEVADTNESKELLKFCRKFTVPLRAALRDAGVLANYETPKRPVVHVFFIAPGCCYT
                     GYSYSSNNSPFYMGIPRLKFPADAPSRSTLKLEEAFHVFIPADEWDERLANGMWAVDL
                     GACPGGWTYQLVKRNMWVYSVDNGPMAQSLMDTGQVTWLREDGFKFRPTRSNISWMVC
                     DMVEKPAKVAALMAQWLVNGWCRETIFNLKLPMKKRYEEVSHNLAYIQAQLDEHGINA
                     QIQARQLYHDREEVTVHVRRIWAAVGGRRDER"
     gene            complement(8846..9577)
                     /gene="fucR"
                     /locus_tag="J1Y83_002585"
     CDS             complement(8846..9577)
                     /gene="fucR"
                     /locus_tag="J1Y83_002585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose operon activator"
                     /protein_id="HBA7630726.1"
                     /translation="MKAARQQAIVDLLLNHTSLTTEALSEQLKVSKETIRRDLNELQT
                     QGKILRNHGRAKYIHRQNQDSGDPFHIRLKSHYAHKADIAREALAWIEEGMVIALDAS
                     STCWYLARQLPDINIQVFTNSHPICHELGKRERIQLISSGGTLERKYGCYVNPSLISQ
                     LKSLEIDLFIFSCEGIDSSGALWDSNAINADYKSMLLKRAAQSLLLIDKSKFNRSGEA
                     RIGHLDEVTHIISDERQVATSLVTA"
     gene            complement(9634..10056)
                     /gene="fucU"
                     /locus_tag="J1Y83_002586"
     CDS             complement(9634..10056)
                     /gene="fucU"
                     /locus_tag="J1Y83_002586"
                     /EC_number="5.1.3.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose mutarotase"
                     /protein_id="HBA7630727.1"
                     /translation="MLKTISPLISPELLKVLAEMGHGDEIIFSDAHFPAHSMGPQVIR
                     ADGLLVSDLLQAIIPLFELDSYAPPLVMMAAVEGDTLDPEVERRYRNALSLQAPCPDI
                     IRINRFAFYERAQKAFAIVITGERAKYGNILLKKGVTP"
     gene            complement(10058..11476)
                     /gene="fucK"
                     /locus_tag="J1Y83_002587"
     CDS             complement(10058..11476)
                     /gene="fucK"
                     /locus_tag="J1Y83_002587"
                     /EC_number="2.7.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417283.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculokinase"
                     /protein_id="HBA7630728.1"
                     /translation="MKQEVILVLDCGATNVRAIAVNRQGKIVARASTPNASDIAMENN
                     TWHQWSLDAILQRFADCCRQINSELTDCHIRGIAVTTFGVDGALVDKQGNLLYPIISW
                     KCPRTAAVMDNIERLISTQQLQAISGVGAFSFNTLYKLVWLKENHPQLLERAHAWLFI
                     SSLINHRLTGEFTTDITMAGTSQMLDIQQRDFSPQILQATGIPRRLFPRLVEAGEQIG
                     TLQNSAAAMLGLPVGIPVISAGHDTQFALFGAGAEQNEPVLSSGTWEILMVRSAQVDT
                     SLLSQYAGSTCELDSQAGLYNPGMQWLASGVLEWVRKLFWTAETPWQILIEEARLIAP
                     GADGVKMQCDLLSCQNAGWQGVTLNTTRGHFYRAALEGLTTQLQRNLQMLEKIGHFKA
                     SELLLVGGGSRNTLWNQIKANMLDIPVKVLDDAETTVAGAALFGWYGVGEFNSPEEAR
                     AQIHYQYRYFYPQTEPEFIEEV"
     gene            complement(11585..13360)
                     /gene="fucI"
                     /locus_tag="J1Y83_002588"
     CDS             complement(11585..13360)
                     /gene="fucI"
                     /locus_tag="J1Y83_002588"
                     /EC_number="5.3.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose isomerase"
                     /protein_id="HBA7630729.1"
                     /translation="MKKISLPKIGIRPVIDGRRMGVRESLEEQTMNMAKATAALLTEK
                     LRHACGAAVECVISDTCIAGMAEAAACEEKFSSQNVGLTITVTPCWCYGSETIDMDPT
                     RPKAIWGFNGTERPGAVYLAAALAAHSQKGIPAFSIYGHDVQDADDTSIPADVEEKLL
                     RFARAGLAVASMKGKSYLSLGGVSMGIAGSIVDHNFFESWLGMKVQAVDMTELRRRID
                     QKIYDEAELEMALAWADKNFRYGEDENNKQYQRNAEQSRAVLRESLLMAMCIRDMMQG
                     NSKLADIGRVEESLGYNAIAAGFQGQRHWTDQYPNGDTAEAILNSSFDWNGVRKPFVV
                     ATENDSLNGVAMLMGHQLTGTAQVFADVRTYWSPEAIERVTGHKLDGLAEHGIIHLIN
                     SGSAALDGSCKQRDSEGKPTMKPHWEISQQEADACLAATEWCPAIHEYFRGGGYSSRF
                     LTEGGVPFTMTRVNIIKGLGPVLQIAEGWSVELPKDVHDILNKRTNSTWPTTWFAPRL
                     TGKGPFTDVYSVMANWGANHGVLTIGHVGADFITLASMLRIPVCMHNVEETKVYRPSA
                     WAAHGMDIEGQDYRACQNYGPLYKR"
     gene            complement(13393..14709)
                     /gene="fucP"
                     /locus_tag="J1Y83_002589"
     CDS             complement(13393..14709)
                     /gene="fucP"
                     /locus_tag="J1Y83_002589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose:H+ symporter permease"
                     /protein_id="HBA7630730.1"
                     /translation="MGNTSIQTQSYRAVDKDAGQSRSYIIPFALLCSLFFLWAVANNL
                     NDILLPQFQQAFTLTNFQAGLIQSAFYFGYFIIPIPAGILMKKLSYKAGIITGLFLYA
                     FGAALFWPAAEIMNYTLFLVGLFIIAAGLGCLETAANPFVTVLGPESSGHFRLNLAQT
                     FNSFGAIIAVVFGQSLILSNVPHQSQDVLDKMSPEQLSAYKHSLVLSVQTPYMIIVAI
                     VLLVALLIMLTKFPALQSDNHSDAKQGSFSASLSRLARIRHWRWAVLAQFCYVGAQTA
                     CWSYLIRYAVEEIPGMTAGFAANYLTGTMVCFFIGRFTGTWLISRFAPHKVLAAYALI
                     AMALCLISAFAGGHVGLIALTLCSAFMSIQYPTIFSLGIKNLGQDTKYGSSFIVMTII
                     GGGIVTPVMGFVSDAAGNIPTAELIPALCFAVIFIFARFRSQTATN"
     gene            15256..15903
                     /gene="fucA"
                     /locus_tag="J1Y83_002590"
     CDS             15256..15903
                     /gene="fucA"
                     /locus_tag="J1Y83_002590"
                     /EC_number="4.1.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculose-phosphate aldolase"
                     /protein_id="HBA7630731.1"
                     /translation="MERNKLARQIIDTCLEMTRLGLNQGTAGNVSVRYQDGMLITPTG
                     IPYEKLTESHIVFIDGNGKHEEGKLPSSEWRFHMAAYQSRPDANAVVHNHAVHCTAVS
                     ILNRPIPAIHYMIAAAGGNSIPCAPYATFGTRELSEHVALALKNRKATLLQHHGLIAC
                     EANLEKALWLAHEVEVLAQLYLTTLAITDPVPVLSDEEIAVVLEKFKTYGLRIEE"
     gene            15931..17079
                     /gene="fucO"
                     /locus_tag="J1Y83_002591"
     CDS             15931..17079
                     /gene="fucO"
                     /locus_tag="J1Y83_002591"
                     /EC_number="1.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactaldehyde reductase"
                     /protein_id="HBA7630732.1"
                     /translation="MANRMILNETAWFGRGAVGALTDEVKRRGYQKALIVTDKTLVQC
                     GVVAKVTDKMDAAGLAWAIYDGVVPNPTITVVKEGLDVFQNSGADYLIAIGGGSPQDT
                     CKAIGIISNNPEFADVRSLEGLSPTNKPSVPILAIPTTAGTAAEVTINYVITDEEKRR
                     KFVCVDPHDIPQVAFIDADMMDGMPPALKAATGVDALTHAIEGYITRGAWALTDALHI
                     KAIEIIAGALRGSVAGDKDAGEEMALGQYVAGMGFSNVGLGLVHGMAHPLGAFYNTPH
                     GVANAILLPHVMRYNADFTGEKYRDIARVMGVKVEGMSLEEARNAAVEAVFALNRDVG
                     IPPHLRDVGVRKEDIPALAQAALDDVCTGGNPREATLEDIVELYHTAW"
     gene            complement(17184..18029)
                     /gene="xni"
                     /locus_tag="J1Y83_002592"
     CDS             complement(17184..18029)
                     /gene="xni"
                     /locus_tag="J1Y83_002592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311685.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flap endonuclease Xni"
                     /protein_id="HBA7630733.1"
                     /translation="MRGLFPISHPAIACSGIECYPYRLIFKGVIVAVHLLIVDALNLI
                     RRIHAVQGSPCVETCQHALDQLIMHSQPTHAVAVFDDENRSSGWRHQRLPDYKAGRPP
                     MPEELHNEMPALRAAFEQRGVPCWSASGNEADDLAATLAVKVTQAGHQATIVSTDKGY
                     CQLLSPTLRIRDYFQKRWLDAPFIDKEFGVQPQQLPDYWGLAGISSSKVPGVAGIGPK
                     SATQLLVEFQSLEGIYENLDAVAEKWRKKLENHKEMAFLCRDIARLQTDLHIDGNLQQ
                     LRLVR"
     gene            complement(18051..19418)
                     /gene="sdaB"
                     /locus_tag="J1Y83_002593"
     CDS             complement(18051..19418)
                     /gene="sdaB"
                     /locus_tag="J1Y83_002593"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase II"
                     /protein_id="HBA7630734.1"
                     /translation="MISVFDIFKIGIGPSSSHTVGPMKAGKQFTDDLIARNLLKDVTR
                     VVVDVYGSLSLTGKGHHTDIAIIMGLAGNLPDTVDIDSIPGFIQDVNTHGRLMLANGQ
                     HEVEFPVDQCMNFHADNLSLHENGMRITALAGDKVVYSQTYYSIGGGFIVDEEHFGQQ
                     DSAPVEVPYPYSSAADLQKHCQETGLSLSGLMMKNELVLHSKEELEQHLANVWEVMRG
                     GIERGISTEGVLPGKLRVPRRAAALRRMLVSQDKTTTDPMAVVDWINMFALAVNEENA
                     AGGRVVTAPTNGACGIIPAVLAYYDKFIREVNANSLARYLLVASAIGSLYKMNASISG
                     AEVGCQGEVGVACSMAAAGLAELLGASPAQVCIAAEIAMEHNLGLTCDPVAGQVQVPC
                     IERNAIAAVKAVNAARMALRRTSEPRVCLDKVIETMYETGKDMNAKYRETSRGGLAMK
                     IVACD"
     gene            complement(19476..20765)
                     /locus_tag="J1Y83_002594"
     CDS             complement(19476..20765)
                     /locus_tag="J1Y83_002594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAAAP family serine/threonine permease"
                     /protein_id="HBA7630735.1"
                     /translation="METTQTSTIASKDSRSAWRKTDTMWMLGLYGTAIGAGVLFLPIN
                     AGVGGMIPLIIMAILAFPMTFFAHRGLTRFVLSGKNPGEDITEVVEEHFGIGAGKLIT
                     LLYFFAIYPILLVYSVAITNTVESFMSHQLGMTPPPRAILSLILIVGMMTIVRFGEQM
                     IVKAMSILVFPFVGVLMLLALYLIPQWNGAALETLSLDTASATGNGLWMTLWLAIPVM
                     VFSFNHSPIISSFAVAKREEYGDMAEQKCSKILAFAHIMMVLTVMFFVFSCVLSLTPA
                     DLAAAKEQNISILSYLANHFNAPIIAWMAPIIAIIAITKSFLGHYLGAREGFNGMVIK
                     SLRGKGKSIEINKLNRITALFMLVTTWIVATLNPSILGMIETLGGPIIAMILFLMPMY
                     AIQKVPAMRKYSGHISNVFVVVMGLIAISAIFYSLFS"
BASE COUNT         5186 a         5111 c         5526 g         5212 t
ORIGIN      
        1 ataaagcagt tgaagaagtt attgattccc ctctttcgga ggtcgcaaat ttaattaaag
       61 tattacaatc ttgtcagggg aaagttgttt ttattggtgt tggtaagtct ggtattattg
      121 ccaggaaact cgccgcaaca tttgccagca ccggaactcc ctcgtttttt gttcacggta
      181 cggaagcggt acacggcgac cttggaatgg tggcgaaaga cgatgttgtt attcttattt
      241 caaacagtgg tgagacggcg gaaatcctgg cgacactgcc tagtctgaaa aaaatgggta
      301 attatttgat ctcttttacc cgcagtcatc actcatcgct tgcgataagt tgtgatttat
      361 ctgttgaaat ccccgtcaaa agtgaggcgg ataatttagg tttagcaccg tcatgctcgt
      421 cgactgttgt tctggttgtt ggtgatgcgg tggcgcttgc actctctgaa ttgaaaaaat
      481 ttactcgtgc tgatttcggc ttatatcatc caggaggtgc actcggcatt aaagcaaatt
      541 cataatttca aaatataaat acagtaaccg gtcacgccat cttttggggt ggcgttcttt
      601 gaagtgagtg tccgctatga aaaatgttct ggtcaccgtc cccagctttt cagcacgctg
      661 tgtttccgcc agcaagttgc taagagagaa taattttaat cttattatta agaataatgt
      721 ggagcatctg cttaaatcag aatccactgc attacgagaa tctatttgcg cggtcatcgc
      781 cggaaaagat ggttatcagg cggacacgct atcattgctg cctggtgtga gaataatatc
      841 gagattcggg acggggatag acaatattga tcttcgtgcg gcacagcaat ccggtattgt
      901 tgtcaataat gcggttggga ttaattcaaa tgcggttgcg gaatttatca ttggacttat
      961 cttcgcgagc atgagaaata tccctggcag ctatcatgcg atgcaaaatg gctactgggg
     1021 tgagtcgcat ggctgtgaat tacaagggaa acggattggc ctggttggct acggtaatat
     1081 aggtaaaact ctggcgaaaa ggttatccgg ttttgatgtt gagcttttag cttttgacaa
     1141 acaacccgac tatcaatttg ctgacaaagc tggagttcag tttgtatcaa ttgaagatat
     1201 ctttatgcag tcgcatgtca tcattgttct tttgcctttt tcttctgagc tggagaactt
     1261 cattagtcat aaatatttgt cgatgatgcg caatggtgct ctgatcatca atgccgcaag
     1321 gggaaagttg ctggatgagg gcgctttact tcaggtgatt gaagaacgaa atgtgtttgc
     1381 ggcgctggat gtgtttagct ctgaaccgtt ggcgcaattt agcccactgc tacatgccaa
     1441 aaatatcatt acaacgccgc atatcgcggc cgccaccgtt gagtcatacc agcaaaccgg
     1501 gatacacgtt gcccagtcga taattgatta cttcgcagga agggagataa aaaacgtgtt
     1561 gtgagttcag cgaacaaaga ttttaaatac aaaaaaaccg caatttgtag aacacattgc
     1621 ggtttgtcag cattctaata aattggttgc gggggccgga tttgaaccga cgaccttcgg
     1681 gttatgagcc cgacgagcta ccaggctgct ccaccccgcg tccgtggatg cgcactatac
     1741 tctgctcgcg ttttcatgca accttttttt taccccgatc acggattcgg catgattttg
     1801 aacaaaaaca aatcactaaa gcctattttt tgtgcaaaat ttgcgtcagg gagaggctgc
     1861 tcatttcatt aaacgcggtg gtttgttatc ttcgttgcgc cttatttttt aacctgaaga
     1921 agagaacaat gaaaggacga tgggtaaagt accttcttat gggcacggtt gtggcaatgc
     1981 ttgccgcctg ctcttccaaa ccaaccgatc gcggacagca atataaagac gggaaattta
     2041 cccagccttt ctctctggtg aaccagccag atgccgttgg cgcgccgatt aatgccggtg
     2101 attttgccga gcaaattaac catatccgta attcgtcacc gcgtctgtat ggcaaccaga
     2161 gtaatgttta taacgcggtg caagagtggc tgcgcgcagg cggtgatacc cgcaatatgc
     2221 gccagttcgg cattgatgcc tggcagatgg aaggtgtcga caactatggt aacgtgcagt
     2281 ttacaggcta ttacacgccg gtaattcagg cgcgccatac ccgtcagggc gagttccagt
     2341 atccgattta ccgtatgccg ccaaaacgtg gtcgtctgcc gtctcgtgcg gagatctacg
     2401 caggagcgct gagtgataaa tatattctcg cttacagtaa ctccctgatg gataacttca
     2461 ttatggatgt gcagggtagc gggtatatcg actttggtga tggcagtccg cttaactttt
     2521 tcagctatgc agggaaaaac ggtcatgcct atcgcagcat tggtaaggtg ttgatcgacc
     2581 gtggcgaagt gaaaaaagaa gatatgtcga tgcaggcgat tcgtcactgg ggcgaaacac
     2641 acagtgaagc tgaggttcgc gagctgctgg aacagaaccc gtctttcgtc ttctttaaac
     2701 cgcaatcttt tgcaccggtg aaaggggcaa gtgcggtgcc gctggttggt cgtgcttctg
     2761 ttgcctccga tcgttcaatt attccgccag gtactacctt gctggcagaa gtgccgctgc
     2821 tggataataa cggcaaattt aacggtcagt acgaattgcg tctgatggtg gcgctggatg
     2881 tcggtggcgc aatcaaaggc caacacttcg atatctatca agggatcggg ccggaagccg
     2941 gacaccgcgc tggttggtac aaccactatg gacgtgtctg ggtgctgaaa accgccccgg
     3001 gcgcaggtaa cgtctttagc ggctgatgtg atattctgta tgccgttttt acggatatga
     3061 cagggtgagg ggtaacctca ccctttttaa ttctgaggtg ctatgtctgt ggtaattagt
     3121 gatgcatggc gtcagcgttt tggtggcaca gcgcgtctgt atggtgaaaa agcattgcaa
     3181 ctgtttgctg acgcgcatat ttgtgtggtt ggtatcggtg gtgtcggttc ctgggcggcg
     3241 gaagcgctag cgcgcacggg gattggcgca atcacgctta tcgatatgga tgatgtgtgc
     3301 gtcaccaata ccaatcggca aattcacgct ctgcgcgata acgtcgggct ggcaaaagcg
     3361 gaagtcatgg cggagcgtat tcgccagatt aacccggagt gccgtgtaat ggtggtggat
     3421 gacttcgtga cgccggataa cgtagcgcaa tatatgaatg ccggttattc gtacgtgatt
     3481 gatgctattg atagtgtacg gcccaaagcg gcgctgattg cttattgtcg gcgcaataaa
     3541 atcccgctgg tcacgaccgg tggcgctggt gggcagattg acccgacgca gattcaggtt
     3601 accgatctgg cgaaaacgat tcaggacccg ttggcggcga agttgcgcga gcgcctgaaa
     3661 agcgattttg gcgtagtgaa aaacagtaaa ggtaagctcg gcgtggattg cgtgttctct
     3721 accgaagcgc tggtgtaccc gcagtcagac ggcaccgtct gtgcgatgaa agccacggcg
     3781 gaagggccaa agcggatgga ttgtgcctca ggatttggcg cggcgacgat ggtgaccgcc
     3841 acttttggct ttgttgcggt ttctcatgcg ctgaagaaga tgatggcgaa agcggcgcgt
     3901 caggggtaaa caatcaggtc ggatgcgacg cgaacgtctt atccgacctg gttctgtgct
     3961 taaacctgct tcgccgcagc gataatcgcc tcgcttaacg cattcaaccc ctggctgcgt
     4021 gaggcgctaa gttgcgcacg taatcccagc tcatcaaaca atgccagcgg cgactgtgcc
     4081 tgcaattcgg cggcggtttt cccctcaacg gcagtcaaca acaccgccaa caggccgcgc
     4141 acaatgcgcc cttcgctgtc gccaaagaaa tgcatcttgc cgttttcagc cacggtatat
     4201 cccagccaga cgcggttttc gcatccggca atctctttag cctgcgcttt taactcctct
     4261 ggcaaagccg gaagctgttt tcccagcatg atcaactggc gatatttatc ttcccattgc
     4321 gtcaacggtg cgaaggtatt gcgtaacgtt tctgcggtta cggttgtgcc gaacggatgt
     4381 ccggcgaatt gcgggtttgt cattaatcca ccaataattc cagcgcgcgg tcaacggcat
     4441 tcaccagcgc atccacatca ctctttgtat tatatggcgc aaatgaggcg cgcagtgtgc
     4501 cggttacgcc taattctgcc agtagcggct gagcgcaatg ctgcccggcg cgcagggcaa
     4561 taccgtactc cgccagcagt gtcaccatat cgctgtgatg aacacccgca aaatcaaagg
     4621 ccagcaggct ggaatcctgg cagcggaatg aacgaaagcc tggacgtttc gccagcgctt
     4681 cttctgcaag cgttgctaag ctacggctcc agctttcggc ctggttaata tcgtaatctg
     4741 tcagccattc cagcgccgcg cttaatccta tgacgccagc gacatttggc gttcctgctt
     4801 ccagtttcca cggcgcagat tgagtcgtga agccttcaaa actcacttcg tgaaccattt
     4861 tgccgccgcc cagccagggc gacatcgctt ccagcagttc tggtttgcca tacagcacgc
     4921 cgatgcctgt cgggccatac agtttgtgac ctgaaaaagc atagaaatca atatccagtt
     4981 gctgaacatc cgcggggaaa tgcaccgccc cctgagcacc atcaaccata accaccatcc
     5041 cggctgaatg agcaaaagta atcgctctcg ccagatccgg gcaaccgcca gtgacgttcg
     5101 acatctggcc caacgccaga atccgactac ggggagtaat cagttctggc aacaaatcga
     5161 catccggcag tcgctgcgca ttaagcggca atttcaccac tttggccccc gtttgttggg
     5221 cgaccatcag ccaggggacg aggttggcgt ggtgttctgc cacactgaca ataatttcat
     5281 cgcccggttg cagacgcgga cgggcatagc attgcgccac catgttgatg gattcagtgg
     5341 tgccgcgcgt ccagacgata tttttgtcat ccgatgcatt cagtaattgt gccacttttt
     5401 cccgcgcagc ttcgtaacgc gcggtcaggc gttgggcttc ggcaaactgg ctgcgatgga
     5461 cgtttccggc gctcaggcta taaaactgtc gggtggcttc aaccacggct tcaggtttaa
     5521 gcgcggtcgc ggcgctgtcg agatagacgc ccgcatcctg tagcgcggga aactgggcgc
     5581 gaaactgcgc gggattaaaa acgttcatgg tactcctcgg cttgatgcac cgatcgtgac
     5641 gcaaaattca ctttgataca aggagagtgc taaccatcag aattatctgg atatcctggc
     5701 gaagccgcgt cagtaacata tgctgaatag tgttaggcgt taattttagc ctgttaatca
     5761 atactattaa gatttattaa tcgctttaag gagaagatga tgaaaaagac tgccgcaatt
     5821 atttctgcct gtatgctgac ttttgccctg agcgcctgtt ccggttcgaa ctatgtgatg
     5881 cacaccaatg acggacgtac catcgtctct gacggcaaac cacagactga taacgatacc
     5941 ggtatgattt cgtataaaga cgctaatggc aacaaacagc agatcaaccg taccgacgtg
     6001 aaagagatgg tcgaactgga tcagtaattc gcgatcgcaa ggtaaaaaaa agcaccgcaa
     6061 ttaggcggtg ctacattaat cactatggac agacagggta aatgtacagg aagtgaaaaa
     6121 aggtagcttt gctaccatgg tctgaatcgc agaccaattg caaacacaac aacacaacat
     6181 cacaaccgta agccaaaagt tcaccagaac acgcattccg ataaaacttt tcgttccggc
     6241 tcaggaagtg ccgccactat aggtatttgc tggtagaagc tcaacggaca atttataatg
     6301 gctcagatta aaaaaactaa taggttacat agtgtaatct aattgttaaa ttcatttaac
     6361 atcaaagttt aaaagccatg tctaaacgat taccaccgct aaatgcctta cgagtttttg
     6421 atgccgcagc acgccattta agtttcactc gcgcagcaga agagcttttt gtgacccagg
     6481 ccgcagtaag tcatcaaatc aagtctcttg aggattttct ggggctaaaa ctgttccgcc
     6541 gccgtaatcg ttcactcctg ctgaccgagg aagggcaaag ctatttcctc gatatcaaag
     6601 agatattttc gcaattaacc gaagcgacgc gtaaactcca ggcccgtagc gctaaggggg
     6661 cgttgacggt cagtttactc cccagtttcg cgattcattg gttggttccg cgactttcca
     6721 gctttaattc agcttatccg ggaattgacg ttcgaatcca ggcggttgat cgtcaggaag
     6781 ataagctggc ggatgatgtt gatgtggcga tattttatgg tcggggcaac tggccggggc
     6841 tacgggtgga aaaactgtac gccgaatatt tattgccggt gtgctcgccg ctactgctga
     6901 ctggcgaaaa acccttgaag accccggaag atctggctaa acatacgtta ttacatgatg
     6961 cgtcacgccg tgactggcag acatataccc gacagttggg gttaaatcat atcaacgttc
     7021 agcaagggcc aatttttagc catagcgcca tggtgctgca agcggctatc cacgggcagg
     7081 gagtggcgct ggcaaataac gtgatggcgc aatctgaaat cgaggccgga cgtcttgttt
     7141 gcccgtttaa tgatgttctg gtcagtaaaa acgcttttta tctggtttgt catgacagcc
     7201 aggcagaact gggtaaaata gccgcctttc gccaatggat cctggcgaaa gccgctgctg
     7261 aacaagaaaa attccgcttt cgttatgaac aataatttac gtagggtacg accatgacca
     7321 gccgttttat gctgattttc gccgccatta gcggcttcat ttttgtagct ctgggcgctt
     7381 ttggcgcgca tgtgttaagt aaaaccatgg gggccgttga gatgggctgg atccagaccg
     7441 gcctcgaata ccaggcgttt catacgctgg cgatcttagg tctggcggtg gcaatgcagc
     7501 gtcgcatcag tatctggttt tactggagta gcgttttcct cgcgttaggc acggtgctgt
     7561 tcagcggcag cctttattgc ctggcgctgt cccatctgcg tttgtgggcg tttgtcacac
     7621 cggttggcgg cgtgagcttc cttgcgggct gggcgttaat gttagttggt gctatccgtt
     7681 taaagcgcaa gggcgtaagt catgaataag gttgtattgc tgtgccgtcc gggctttgaa
     7741 aaagagtgcg ccgcagaaat taccgataaa gccggccagc gggaaatttt cggttttgcc
     7801 cgcgtgaaag agaatgcggg ttatgtgatt tatgaatgtt atcaacctga tgatggcgat
     7861 aagttaatcc gtgagctgcc gttcagttca ttaatttttg cccgccagtg gtttgtagtg
     7921 ggggaactcc tgcagcattt gccgccagaa gatcgtatta cccccattgt tggcatgtta
     7981 cagggcgtag tagagaaggg cggtgaactg cgtgttgaag ttgccgatac caacgaaagc
     8041 aaagagttgc tgaaattctg ccgtaaattc accgtgccgc tacgcgctgc cttgcgtgat
     8101 gcgggggtgc tggcgaacta tgaaacgccg aagcgtccgg ttgtgcatgt attctttatt
     8161 gcaccaggct gttgctatac tggttactca tacagcagca ataattcgcc gttctatatg
     8221 ggcattccgc gcctgaaatt tccggcagat gcgccgagtc gttccacgct caaactggaa
     8281 gaggcatttc atgtgtttat tcccgcggat gagtgggatg aacgcctggc gaacgggatg
     8341 tgggcggtgg atttgggcgc ttgcccaggc ggctggacct accaattggt gaagcgcaac
     8401 atgtgggttt attccgtcga caacggcccg atggcgcaaa gtctgatgga taccgggcag
     8461 gtgacgtggc tgcgggaaga cggtttcaaa ttccgtccga cacgcagcaa tatctcctgg
     8521 atggtatgcg atatggttga aaaaccggcg aaagttgcgg cattaatggc gcagtggctg
     8581 gttaatggct ggtgccgtga aactatcttc aatctcaaac tgccgatgaa aaaacgctac
     8641 gaagaagtgt cacacaatct ggcgtatatt caggcacagc ttgatgaaca tggcataaat
     8701 gctcagattc aggcgcggca gttgtatcac gatcgcgaag aagtgacggt gcacgtccgc
     8761 cgtatctggg ctgcggtggg tggtcgtcgc gacgagcgat aacaggcaag gccggggcgt
     8821 cgtctccggc ctgctaccct ttccgtcagg ctgttaccaa agaagttgca acctggcgct
     8881 catcagaaat aatgtgcgtt acctcatcca gatgcccgat gcgggcttcc cctgaacgat
     8941 taaatttact tttatcaatc aataacaacg attgcgcggc acgttttaat aacattgatt
     9001 tgtaatcagc gttgatggca ttggagtccc acagtgcgcc gctgctatcg atcccttcac
     9061 aagaaaaaat aaacagatcg atttccagcg atttaagctg ggaaatcagc gagggattga
     9121 cgtaacaacc atatttgcgc tcaagtgtgc cgccggaact gatcagttga atgcgttcgc
     9181 gtttaccgag ttcatggcaa atcggatggc tattggtgaa gacctggatg ttgatgtcag
     9241 gcaactggcg tgccagatac cagcaagttg aactggcgtc taaggctatc accatccctt
     9301 cttcaatcca cgcgagcgcc tcgcgcgcga tatctgcttt atgcgcataa tggcttttca
     9361 gccggatgtg aaagggatcg ccactgtctt gattttgacg gtggatatat ttagcgcgcc
     9421 catgattgcg cagaatttta ccctgcgtct gtaattcatt gagatcgcga cgaatggttt
     9481 ctttactgac ctttagctgt tcagagagag cttccgtggt caggctggta tggttcagca
     9541 gcaggtcgac tatcgcttgc tggcgtgccg ctttcatatt ttcaccccat cattcgataa
     9601 ccgcccgcgt catgcgggcg taccggtatg agattacggt gttacccctt tttttaaaag
     9661 aatattcccg tacttcgctc gttcgcctgt gataacgatc gcaaaggctt tttgcgcccg
     9721 ttcataaaac gcaaaacgat tgatgcggat gatgtcagga cagggggctt gtagtgaaag
     9781 cgcattacga taacgtcgtt ctacttcagg atcgagagtg tcaccttcta ccgccgccat
     9841 catcaccagc ggtggtgcat aactgtccag ctcaaataac gggataatcg cctggagcaa
     9901 gtcgctcacc aacaggccat cagcgcggat cacctgcggt cccatcgaat gggcgggaaa
     9961 gtgagcatcg gaaaaaataa tttcatctcc atgccccatc tctgccagca ctttcaatag
    10021 ttcgggagaa attaacggcg aaattgtttt cagcatttca cacctcctct ataaattcag
    10081 gttcagtttg cgggtagaaa taacggtact gataatgaat ctgtgcacgg gcttcttccg
    10141 ggctgttaaa ttcccctacg ccataccaac caaacagcgc agctcctgcg acggtcgttt
    10201 cggcatcgtc gagaactttt accggaatat caagcatatt ggctttaatc tgattccaca
    10261 atgtgttgcg acttcctccg ccgactaaca ataattcaga ggctttaaag tgcccgattt
    10321 tttccagcat ctgtagattg cgctgtaatt gcgtggttaa cccttccagc gccgcgcgat
    10381 agaaatgccc ccgcgtggta ttaagcgtta cgccttgcca gccagcgttc tggcacgaca
    10441 ataaatcaca ctgcattttc actccatctg cgccaggcgc gatcaggcga gcttcttcaa
    10501 tcaatatttg ccagggagtt tcagctgtcc agaacagttt tctcacccat tccagcacgc
    10561 cggatgccag ccattgcata cctgggttat acaaccctgc ctggctatcc agttcgcaag
    10621 tagaaccggc gtactgactt aacagcgaag tatcaacctg ggcgctgcgg accattaaaa
    10681 tttcccatgt accggaagag agcacgggtt cattttgttc ggcaccagcg ccaaaaaggg
    10741 cgaactgggt atcgtgacct gcggaaatca ccggtatgcc aacgggtaag ccgagcattg
    10801 ctgcggcgct gttctgtagc gtaccaatct gttcacccgc ttccaccaga cgagggaaga
    10861 gtcggcgtgg aataccggtg gcttgtaaaa tttgcggact gaaatcgcgt tgctggatat
    10921 ccagcatctg gctggttccg gccatcgtaa tatcagtagt gaattcgccg gttaaacggt
    10981 ggttaatcag cgacgaaata aagagccagg cgtgcgcgcg ttccagcagt tgtggatgat
    11041 tttctttcaa ccacaccaac ttatataacg tattgaaact aaaggctccg actccagaaa
    11101 tagcctgcaa ctgctgtgtg gagatcaacc gttcaatatt gtccattacc gcggctgttc
    11161 gcggacattt ccagctaatg atcggataga gcagattgcc ttgcttatct accagagcgc
    11221 catccacacc aaaggtggtg acggcgatac cgcggatgtg gcaatcagtc agttcactat
    11281 tgatttgccg acagcaatca gcaaagcgtt gcaaaatggc gtctaaagac cactggtgcc
    11341 aggtgttgtt ttccatcgcg atatcgctgg cattaggcgt tgaggcgcgg gcaacaattt
    11401 ttccctgccg attaaccgcg atggccctga cattggtcgc gccacagtcg agtaccagga
    11461 taacttcttg tttcataatc gctcctgcaa tatagccgga taacattgct tatccggcta
    11521 accactcttg cttgttcagg cccggtagcc ggagcgaccg ggcatcacat cagggagtca
    11581 tgtattaacg cttgtacaac gggccgtagt tctggcaagc gcggtaatcc tggccttcaa
    11641 tatccatgcc gtgcgcggcc caggcagaag gacgatacac tttggtctct tcaacgttgt
    11701 gcatacatac cgggatacgc agcatggagg caagagtgat aaagtctgcg ccaacgtggc
    11761 cgatggtcag aactccgtgg ttagcgcccc agttcgccat taccgagtac acatccgtaa
    11821 acggcccttt accggtgagg cgcggtgcaa accaggtggt tggccaggtt gagttggtgc
    11881 gtttgttgag gatgtcatgc acatccttcg gcaattccac gctccagcct tccgcgattt
    11941 gcagtaccgg tcccaggcct ttgatgatgt tgacacgagt catggtgaac gggacgccgc
    12001 cttcggtaag gaagcgggaa gagtaaccgc cgccacggaa gtattcgtgg atcgccgggc
    12061 accattcggt agcggcgagg caagcgtcag cctcttgctg agagatttcc cagtgcggct
    12121 tcatcgtcgg tttaccttcg ctgtcgcgtt gtttacagga gccgtccagc gcagcagaac
    12181 cggagttgat caaatggatg atgccgtgtt ctgccagccc gtccagttta tgccccgtta
    12241 cacgctcaat tgcttctggt gaccagtagg tacggacatc ggcaaatacc tgagcggtgc
    12301 cggtgagctg gtggcccatt aacattgcca cgccgttaag actgtcgttt tcggtcgcca
    12361 cgacaaacgg tttgcgcacg ccattccagt caaatgaact gttgaggatc gcttcagcgg
    12421 tatcgccatt gggatattga tcggtccagt gacgttgccc ctggaagcct gcagcgatgg
    12481 cgttgtagcc aagtgattct tccacgcgac caatatcggc cagtttgctg ttgccttgca
    12541 tcatgtcgcg gatacacatc gccatcagta aactttcgcg cagaactgcg cggctttgct
    12601 cggcattgcg ttgatactgt ttgttatttt catcttcgcc atagcggaag tttttatcag
    12661 cccaggccag cgccatttcc agttcggctt cgtcgtaaat cttctgatcg atacggcgac
    12721 gcagttcggt catatccacc gcctggactt tcattcccag ccaggattca aagaagttgt
    12781 gatcaacgat ggaaccggcg atacccatcg aaacgccgcc cagcgacaga tagcttttac
    12841 ctttcatgct ggcgactgct aaaccggcgc gggcaaagcg cagcagtttt tcttcaacat
    12901 cggcaggaat cgatgtgtca tcggcatcct gaacgtcatg accgtaaatg gagaatgccg
    12961 ggatgccttt ctggctgtga gctgccagag ccgctgccag gtaaacagcg ccgggacgtt
    13021 cagtgccgtt aaagccccaa atggctttcg ggcgggttgg atccatatcg atggtttcac
    13081 tgccatagca ccagcaaggg gttaccgtaa tggtgaggcc tacattctga ctgctgaatt
    13141 tttcttcgca agcagcggct tcagccatac ccgcgataca ggtatcggaa atgacacact
    13201 cgacggcagc tccacaggca tggcgcagtt tctcggtcag cagtgcagcc gtggctttcg
    13261 ccatattcat tgtttgttct tcaagcgact cacgaacacc catgcgacga ccgtcaataa
    13321 ccgggcggat accaattttc ggtaagctga tttttttcat tactgattcc tcactttatt
    13381 cggaaaatat gttcagttag ttgccgtttg agaacggaaa cgggcaaaga taaagatgac
    13441 tgcgaagcag agtgccggga tcagttcggc agtggggatg ttgcccgctg cgtcactgac
    13501 aaaccccatg accggagtga caataccgcc gccaataatg gtcataacga tgaaggacga
    13561 accgtatttg gtgtcctggc cgagattctt aatgcccagc gagaagattg ttgggtactg
    13621 aatcgacata aaggcactgc ataaagtcag ggctattaaa cccacatgac cgccagcgaa
    13681 ggctgagatc aggcacagtg ccatagcgat taatgcgtag gctgccagga ctttgtgtgg
    13741 tgcgaagcga ctgatgagcc aggtaccggt gaaacgacca ataaagaagc acaccatggt
    13801 tccggttaaa tagttagcgg caaagcctgc tgtcatacct ggaatttctt ctacagcgta
    13861 gcgaattaaa tagctccagc aggccgtttg tgcgccaacg tagcagaatt gcgctaatac
    13921 tgcccagcgc cagtggcgaa tacgcgccag gcgagaaagc gatgcggaga acgatccttg
    13981 tttggcgtca ctgtgattat cactctgcaa tgccgggaat ttcgtcagca tgatcagcag
    14041 ggcgaccagt aacacgatag ccacgatgat catataaggt gtctgtaccg ataataccag
    14101 gctgtgttta tacgcactca attgctctgg agacatttta tcgagaacgt cttgcgattg
    14161 atgtggcacg ttagacaaaa taagactttg cccaaagaca accgcgataa ttgcgccaaa
    14221 tgagttaaat gtttgcgcaa gatttaagcg gaagtgacca ctgctttccg gccctaatac
    14281 cgtaacaaaa gggtttgcgg cagtttccag acaacctaat ccggctgcaa taataaatag
    14341 gccaactaaa aacaaggtgt agttcattat ttctgcggcg ggccagaata atgcagcgcc
    14401 gaaggcatat aaaaacaatc cggtaataat ccctgcttta taactgagtt ttttcatcaa
    14461 tatcccggca gggattggga taatgaaata accaaagtaa aaggccgatt ggatcaggcc
    14521 agcctggaaa tttgtcagcg taaaagcctg ctggaattga ggcaataaaa tgtcgttaag
    14581 gttattggct accgcccaaa gaaaaaacag tgagcacagc agcgcgaatg gaataatgta
    14641 acttctgctt tgccctgcat ctttatctac cgcacggtaa ctctgcgttt gtattgatgt
    14701 gtttcccata gcatcctctt aggttcagaa gcttaagcac gtaatcggtt atgttttcaa
    14761 aagtgcaaaa ccagcgagtt cgggcttcat atcagtgaat atttgcctgt ctgaaatgaa
    14821 ctcatggatt cattttcaaa taaaaaacgc tctgtaatat gacggcggtc acacttatta
    14881 gccggaaggt gactttatgt gactaccatc actttaataa tcgaaatttc atgcccgttt
    14941 cagaaaataa atcataaaaa acacaaacca gatgaccgta ttaattgttt gtgccgaaaa
    15001 cgggcattcc tggcagggat gagtagaaaa atgaccgttt tgataaatat ttttaagagc
    15061 ggtcattttt aatggggtta taaaaagaga acttgcagtt atttactgtg attagttgaa
    15121 ccaggtcaca aaatataagc ttgtgaattt ctttctggtt aatcaggaag aagatggtgg
    15181 gtaattaaac ggctaattca atagtgtgaa aggaacaaca ttattgccct gttttgaatc
    15241 agagagaggt agctaatgga acgaaataaa cttgctcgtc agattattga cacttgcctg
    15301 gaaatgactc gcctggggct gaaccagggg acagcgggta acgttagtgt acgttatcag
    15361 gatgggatgc tgattacgcc tacaggtatt ccatatgaaa aactgacgga gtcgcatatt
    15421 gtctttattg atggcaacgg caaacatgag gaaggaaagc tcccctcaag cgagtggcgt
    15481 ttccatatgg cggcctatca aagcagaccg gatgccaacg cggttgttca caatcatgcc
    15541 gttcattgta cggcagtttc cattcttaac cgaccgatcc ccgctattca ctacatgatt
    15601 gcggcggctg gcggtaattc tattccttgc gcgccttatg cgacctttgg aacacgcgaa
    15661 ctttctgaac atgttgcgct ggctctcaaa aatcgtaagg caactttgtt acaacatcat
    15721 gggcttatcg cttgtgaggc gaatctggag aaagcgttat ggctggcgca tgaagttgaa
    15781 gtgctggcac aactttacct gacgaccctg gcgattacgg acccggtgcc agtgctgagc
    15841 gatgaagaga ttgccgtagt gctggagaaa ttcaaaacct acgggttacg gattgaagag
    15901 taatttcgta aagcaacaag gagaaggatg atggctaaca gaatgattct gaacgaaacg
    15961 gcatggtttg gtcgaggtgc tgttggagct ttaaccgatg aggtgaaacg ccgtggttat
    16021 cagaaggcgc tgatcgtcac cgataaaacg ctggtgcaat gcggcgtggt ggcgaaagtg
    16081 accgataaga tggatgcggc aggactggca tgggcgattt acgacggcgt agtacctaac
    16141 ccaacaatta ctgtcgtcaa agaagggctc gatgtattcc agaatagcgg cgcggattac
    16201 ctgatcgcta ttggtggtgg ttctccacag gatacgtgta aagcgattgg cattatcagc
    16261 aacaacccgg agttcgccga tgtgcgtagc ctggaagggc tttccccgac caataaaccc
    16321 agtgtaccga ttctggcaat ccccaccaca gcaggcactg cggcagaagt gaccattaac
    16381 tacgtgatca ctgacgaaga gaaacggcgc aagtttgttt gcgttgatcc gcatgatatc
    16441 ccgcaggtgg cgtttattga cgctgacatg atggatggta tgcctccagc gctgaaagcc
    16501 gcgacgggtg tcgatgcgct cactcatgct attgaggggt atattacccg tggcgcgtgg
    16561 gcgctaaccg atgcgctgca cattaaagcg attgaaatca ttgctggggc gctgcgagga
    16621 tcggttgctg gtgataagga tgccggagaa gaaatggcgc tcgggcagta tgttgcgggt
    16681 atgggattct cgaatgttgg gttagggttg gtgcatggta tggcgcatcc actgggcgcg
    16741 ttttacaaca cgccacacgg tgttgcaaat gccatcctgt taccgcatgt catgcgctat
    16801 aacgctgact ttaccggtga gaagtaccgc gatatcgcgc gcgttatggg cgtgaaagtg
    16861 gaaggtatga gcctggaaga ggcgcgtaat gccgctgttg aagcggtgtt tgctctcaac
    16921 cgtgatgtcg gtattccgcc acatttacgt gatgttggtg tacgcaagga agacattccg
    16981 gcactggcgc aggcggcact ggatgatgtt tgtaccggtg gcaacccgcg tgaagcaacg
    17041 cttgaggata ttgtagagct ttaccatacc gcctggtaaa tgcgctgatg tgataatgcc
    17101 ggatgcgacg ctagccgcgt cttatccggc atataagtgc attaaattca atgtgttgat
    17161 aatcgtttgc atccggcacg ccgttacctt accaaccgca attgctgtaa attgccgtcg
    17221 atatgcaaat cggtttgtaa gcgggcaata tcgcggcaca gaaacgccat ctctttatgg
    17281 ttttctaatt ttttgcgcca cttttcggca accgcatcca gattctcata tatcccttcc
    17341 agactctgaa actcgaccag tagctgtgtg gcgctttttg gtccgatccc cgcaacaccc
    17401 ggtacctttg aactgctgat ccccgcaagc ccccagtaat cgggcaactg ctgcggttga
    17461 acgccaaatt ctttatcgat aaatggcgca tccagccaac gtttctggaa gtaatcacga
    17521 atacgtaatg tcggtgaaag taactgacag tagcctttat ctgtcgaaac aatcgttgcc
    17581 tgatgcccgg cctgtgtcac tttgaccgcc agcgtggcgg ctaagtcatc ggcttcgttg
    17641 ccgctggcgg accagcacgg gacgccgcgt tgctcaaagg cggcgcgtaa tgcaggcatc
    17701 tcgttgtgca actcttccgg cattggcggt cgacccgctt tgtaatctgg taaacgctga
    17761 tgacgccagc cgctactgcg gttttcatca tcaaaaacgg cgaccgcgtg ggttggctgg
    17821 ctgtgcataa tgagctgatc gagcgcgtgc tggcaggttt cgacacaggg tgaaccctga
    17881 acggcatgaa tgcgacgaat aagattcagt gcatcgacaa taagcaaatg aacagccacg
    17941 ataaccccct taaagatcag gcgataaggg taacattcga tgccagaaca ggctatggct
    18001 ggatgagaaa tcgggaagag gcctcgcaaa aagaggcctc tggagagcga ttaatcgcag
    18061 gcaacgatct tcattgccag gccgccgcga gacgtttcac ggtacttggc gttcatatct
    18121 ttacctgttt cgtacatagt ttcgataact ttatcgaggc agacgcgcgg ctcgctggta
    18181 cggcgcagcg ccatacgtgc agcgtttact gctttcactg ccgcaatggc gttacgctcg
    18241 atgcacggca cctgtacctg cccggcgacc gggtcacacg tcagaccgag gttgtgctcc
    18301 atggcgattt ccgccgcgat gcatacctgc gccgggcttg cgcctagcag ttctgccaga
    18361 ccagccgccg ccattgagca cgccacgccg acttcaccct ggcagccgac ttcggcacca
    18421 gaaatcgagg cgttcatctt ataaagagaa ccaatggcgc tggctaccag caggtaacga
    18481 gccagtgagt tagcgttcac ttcgcggata aacttgtcgt agtacgccag cacggccggg
    18541 ataatcccgc acgcaccgtt agtcggcgca gtcaccacgc gaccgccagc agcgttctct
    18601 tcgttcactg ccagtgcaaa catgttgatc cagtcaacaa ccgccatcgg gtcagtggtg
    18661 gttttatcct ggctgaccag catccggcgt agtgccgcag cacggcgtgg aacgcgcagt
    18721 ttgccaggta acacgccttc ggtagaaata ccgcgctcaa taccgccacg catgacttcc
    18781 cagacgttag ccaggtgctg ttccagctct tctttgctgt gcagcaccag ctcgtttttc
    18841 atcatcaggc cagagagtga cagcccggtt tcctgacaat gtttttgcag atcggctgct
    18901 gaactgtacg gataaggaac ttcaaccggt gcgctatcct gctgaccaaa atgttcttca
    18961 tcaacaataa agccaccgcc gatagagtaa taagtctggc tgtaaacgac tttatcgccc
    19021 gccagcgcgg taatgcgcat accgttttca tgcagagaaa ggttgtcggc atggaagttc
    19081 atgcactgat caaccgggaa ctccacttca tgctgaccgt ttgccagcat caggcgacca
    19141 tgagtattca catcctgaat aaaaccaggg atggaatcga tatccacggt atccggcagg
    19201 ttacccgcca gacccataat tatggcgata tctgtgtggt ggcctttacc ggtcagagag
    19261 agcgagccgt acacgtcaac caccacgcgg gtcacgtctt taagcaggtt acgggcaatc
    19321 agatcgtcgg taaattgttt acccgctttc attggtccaa cggtatgaga actggaaggg
    19381 ccaatgccga ttttgaaaat atcgaatacg ctaatcatag gaaatacatc gcgttaaaac
    19441 ggaggaagcg ccgcccgaaa gcggcgcgaa aggacttagc tgaacagaga gtagaagatt
    19501 gcggagattg caatcagacc catcacgaca acgaatacgt tgctgatgtg accgctgtac
    19561 ttacgcattg ccggtacttt ctggattgcg tacatcggca tcaggaacag gatcatcgcg
    19621 atgattgggc cgcccagggt ttcaatcata cccaggatgc tcgggttcag ggtggcaaca
    19681 atccaggtcg ttaccagcat gaacagcgca gtgatacggt tcagcttgtt gatttcgata
    19741 gacttacctt taccacgcag agatttaatc accataccgt tgaagccttc acgtgcgccc
    19801 aggtagtggc cgaggaagga tttggtgata gcgataatcg cgataatcgg agccatccac
    19861 gcgatgatcg gcgcgttaaa gtggttagcc aggtaagaca gaatcgagat gttctgctct
    19921 ttagccgcag ccaggtctgc cggagtcagg ctcagtacgc agctgaagac gaagaacatt
    19981 acggtcagca ccatcatgat gtgtgcgaat gccaggatct tcgagcattt ctgttctgcc
    20041 atatcgccgt actcttcacg cttcgcaacg gcgaaagaag agatgatcgg agagtggttg
    20101 aacgagaaca ccattaccgg aattgccagc cacagtgtca tccacagacc gtttccggtt
    20161 gcagatgcag tgtccagaga cagcgtttcc agtgctgcgc cgttccactg cgggatcagg
    20221 tacagagcca gcagcatcag tacgccaaca aacgggaata ccagaatact catcgcttta
    20281 acgatcatct gctcaccgaa gcgaacgatg gtcatcatac ccacgatcag gattagcgac
    20341 agaatcgcac gcggcggtgg cgtcataccc agctggtgag acatgaagct ttcaactgta
    20401 ttggtgattg ccacgctata aaccagcagg atcgggtaga tagcgaagaa gtagagcaga
    20461 gtaatcagtt tacctgcgcc aataccaaag tgttcttcta caacctcggt gatgtcttcg
    20521 cccgggtttt taccagacag tacgaagcga gtcaggccgc ggtgagcaaa aaacgtcatc
    20581 gggaacgcaa ggatagccat gatgatcagc gggatcatac cgccaacacc agcgttgatt
    20641 ggcaggaaca gcacgcccgc gccgattgcc gtgccgtaaa ggcccagcat ccacatggtg
    20701 tctgtcttgc gccaggcact acgagagtct ttcgacgcaa tcgtgctggt ttgagtcgtt
    20761 tccatctatt tctcctggag gaatgcaaat aattcagctt ttagccagtt cagcccggga
    20821 aggtgcctga ccgtaaaaat gaaatctgtc tatcggcgat ggcttaatca ttttccaccg
    20881 taataatttg agggcggaaa gatacacaca agttatgtat ctatcagtga tcttgatccc
    20941 aaatacaata atctacttgg aagatagata atcacaacgc tttactctgt aaatcaaaag
    21001 taaaagagaa tttaagggca atatttaccc attaa
//
LOCUS       DADSYM010000081        20952 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2415
            SAMN08039579-rid11723173.denovo.081, whole genome shotgun sequence.
ACCESSION   DADSYM010000081 DADSYM010000000
VERSION     DADSYM010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039579
            Sequence Read Archive: SRR6362533
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 20952)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20952)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980162.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 03:39:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,484
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 4,347
            CDSs (with protein)               :: 4,347
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 43 of 85
            Pseudo Genes (incomplete)         :: 37 of 85
            Pseudo Genes (internal stop)      :: 19 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20952
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039579-rid11723173.denovo.081"
                     /strain="LGE2415"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8302 N 95.9628 W"
                     /collection_date="2015-08-24"
     gene            13..1035
                     /locus_tag="J1Y83_002595"
     CDS             13..1035
                     /locus_tag="J1Y83_002595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter substrate-binding protein"
                     /protein_id="HBA7630736.1"
                     /translation="MSLTRRRFTQILASTLFLHHLPSFAQSVKFWVSRTLPEAQNITR
                     IVSAGAPADLLLLAVAPEKMVGFSSFDFARQALIPLPEHIRQFPRLGRLAGRASTLSL
                     EGLMALHPDLVVDCGNTDETWISQARQVSEQTQIPWLLLNGKLEQSAEQLTTLGKTLG
                     EEHRAAEQANLASRFVGEAQAFATSPAANLSFYAARGPRGLETGLQGSLHTEAAELLG
                     LHNVAQIADRHGLTQVSMENLLRWQPDIILVQEAVTADFIRRDSLWQGVKAVAEQRIL
                     FLSGLPFGWLDAPPGINRLLGLRRLHAWLDPAINRQFKSDIQHYAQLFWHCSLSDADY
                     QKLVAS"
     gene            1035..2014
                     /locus_tag="J1Y83_002596"
                     /pseudo
     CDS             1035..2014
                     /locus_tag="J1Y83_002596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309725.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
     gene            2011..2769
                     /locus_tag="J1Y83_002597"
     CDS             2011..2769
                     /locus_tag="J1Y83_002597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBA7630737.1"
                     /translation="MTLLAVRHASLGYSRHPVLRDVSFTLSQGTICCLLGANGCGKTT
                     LMRSILGVIPLLKGEILLDSLSVQTLSHRQRAQAIAWVPQAHDGIFAFSVLDMVLMGL
                     APTIGAFSVPGKPERLKATEQLEKLGILHLAARRWNTLSGGERQLALIARALVQQPRL
                     LLLDEPASSLDFGHQIQLLDTLAQLKNNGMTMLMSTHHPLHANAIADSIIQVEPDGRV
                     TQGLPTEQLTTKKLAALYRVSADQIHHHLSAIKH"
     gene            2779..3591
                     /locus_tag="J1Y83_002598"
     CDS             2779..3591
                     /locus_tag="J1Y83_002598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000904017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HBA7630738.1"
                     /translation="MLIDDIDFADLYLQQLRLAHRTEKTPDHWDQRAEKMAENCASPT
                     DSYLQQLTSKIDLQGAQTLFDMGCGPGTVSLALADKLTTIYGVDYSQGMLNVAARRAA
                     ALKADNVHWIQRAWEEDWSDLPRCDIAVASRSTLVADMRQAMSKLNNQARLRVYTTHL
                     VSTSFVSPAIQRAAGREVIELPNYIFALNVLYQMGIYAHVDFIRGQNCQQDNSTWERF
                     EQNVSWSLGALNDDERERLYRWYQQQDARALAPASRDWALIWWDSVPQETLR"
     gene            3588..4442
                     /locus_tag="J1Y83_002599"
     CDS             3588..4442
                     /locus_tag="J1Y83_002599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ModD protein"
                     /protein_id="HBA7630739.1"
                     /translation="MIFLSQAQIDALLLEDIQGGDLTTRALNIGHQHGYIEFFLRQGG
                     CVSGISVACKMLTTLGLTIDDAVSDGSQANAGQRLIRAQGNAAALHQGWKAVQNVLEW
                     SCGVSDYLAQMLALLRERYPDGNIACTRKAIPGTRLLASQAILAAGGLIHRAGCAETI
                     LLFANHRHFLHDNQDWSGAINQLRRHAPEKKIVVEADTPKEAIAALRAQPDVLQLDKF
                     SPQQATEIAQIAPSLAPHCTLALTGGINLTTLKNYLDCGIRLFITSAPYYAAPADIKV
                     SLQPAASI"
     gene            4468..6438
                     /locus_tag="J1Y83_002600"
     CDS             4468..6438
                     /locus_tag="J1Y83_002600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HBA7630740.1"
                     /translation="MRLKKRYLCTVLSLAFTQQSVAAQESDTLTVWSSPVSSTTTTVL
                     DQPTMKALDKQNVAQALSVVPGVVLQKSGSRNEEQVKVRGFDSRQVPVYFDGVPIYVP
                     YDGNLDLARILTNNLGAVEVSKGYSSLLQGPNQMGGAINITTQKPTKPLEANLGYRQG
                     WSRSQDNAYDMHASFAASSDLGYLQVSGSQLKQDFLGLPHGVNNDIAGKHGKMINSSA
                     DDKRGIVKLGFTPRENDEYTLTYIKQDGEKDNPPYSGNSGQKSRYWQWPEYDKESFYY
                     QGTTQLNDRFTLKSRLYRDTFENTLMMYNSLADLKNKKGSYSHYSDYSDGAGLQLAAD
                     VRENDLLSFAVNWKDDVHREKGAPHAAYDRYEDRTWSLASEYQWAAADNVDVVAGISY
                     DWRDSVEAKKHEKDGSITHYDDNYQSAFNWQVMGKYHFANEDTLALSYYDRTRFPTLK
                     ERYTTSKPAYNQIAIVNPQLKPERARGVDLTWNGAFTHDWGFEVSVYYNRVSDAILSH
                     NIDADTIQNQNSGTVDYSGLDAGIKGKISNILDVGLSYALIHADAKRKDIGKITDLPT
                     QTMTAWMTLKPWEPLSVTLSEEARSSSYSNSDGSQKAAGFAVTHIRADYTLGHGFSVN
                     ASVNNLFDTKYAYSEGFIEEGRNFWAGIEYTF"
     gene            complement(6488..6742)
                     /locus_tag="J1Y83_002601"
     CDS             complement(6488..6742)
                     /locus_tag="J1Y83_002601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HBA7630741.1"
                     /translation="MGIIAWIIFGLIAGIIAKLIMPGRDGGGFFLTCILGIVGAVVGG
                     WLATMFGIGGSISGFNLHSFLVAVVGAILVLGVFRLLRRE"
     gene            6943..7677
                     /locus_tag="J1Y83_002602"
     CDS             6943..7677
                     /locus_tag="J1Y83_002602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar brake protein"
                     /protein_id="HBA7630742.1"
                     /translation="MSHYHEQFLKQNPLAVLGVLRDLNKAAIPLRISWNGGQLISKIL
                     AITPDKLVLDFGSQAEDNIAVLKAQHITITAETQGAKVEFTVEQLQQSEYLLLPAFIT
                     VPPPTLWFVQRRRYFRITAPLHPPYFCQTKLADNSTLRFRLYDLSLGGMGALLETAKP
                     AELHEGMRFAQIEVNMGQWGVFHFDAQLISISERKVIDGKNETITTPRLSFRFLNVSP
                     TVERQLQRIIFSLEREARKKADNVRD"
     gene            complement(7679..8290)
                     /gene="emtA"
                     /locus_tag="J1Y83_002603"
     CDS             complement(7679..8290)
                     /gene="emtA"
                     /locus_tag="J1Y83_002603"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     EmtA"
                     /protein_id="HBA7630743.1"
                     /translation="MKLRWFAFLIVLLAGCSSKHDYTNPPWNAKVPVQRAMQWMPISQ
                     KAGAAWGVDPQLITAIIAIESGGNPNAVSKSNAIGLMQIKASTSGRDVYRRMGWSGEP
                     TTSELKNPERNISMGAAYLNILETGPLAGIEDPKVLQYALVVSYANGAGALLRTFSSD
                     RKKAISKINDLDADEFLDHVARNHPAPQAPRYIYKLEQALDAM"
     gene            8390..9304
                     /gene="ldcA"
                     /locus_tag="J1Y83_002604"
     CDS             8390..9304
                     /gene="ldcA"
                     /locus_tag="J1Y83_002604"
                     /EC_number="3.4.17.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="muramoyltetrapeptide carboxypeptidase"
                     /protein_id="HBA7630744.1"
                     /translation="MSLFHLIAPSGYCIKQHAALRGIQRLTDAGHQVNNVEVIARRCE
                     RFAGTETERLEDLNSLARLTIPNTIVLSVRGGYGASRLLADIDWQALVARQQHDPLLI
                     CGHSDFTAIQCGLLAQGNVITFSGPMLVANFGADELNAFTEHHFWLALRNKTFTIEWQ
                     GEGPTCQTEGTLWGGNLAMLISLIGTPWMPKIENGILVLEDINEHPFRVERMLLQLYH
                     AGILPRQKAIILGSFSGSTPNDYDAGYNLESVYAFLRSRLSIPLITGLDFGHEQRTVT
                     LPLGAHAILNNTQEGTQLTISGHPVLKM"
     gene            9399..11135
                     /locus_tag="J1Y83_002605"
     CDS             9399..11135
                     /locus_tag="J1Y83_002605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium/proton antiporter"
                     /protein_id="HBA7630745.1"
                     /translation="MDATTIISLFILGSILVTSSILLSSFSSRLGIPILVIFLAIGML
                     AGVDGVGGIPFDNYPFAYMVSNLALAIILLDGGMRTQASSFRVALGPALSLATLGVLI
                     TSGLTGMMAAWLFNLDLIEGLLIGAIVGSTDAAAVFSLLGGKGLNERVGSTLEIESGS
                     NDPMAVFLTITLIAMIQQHESSVSWMFVVDILQQFGLGIVIGLGGGYLLLQMINRIAL
                     PAGLYPLLALSGGILIFALTTALEGSGILAVYLCGFLLGNRPIRNRYGILQNFDGLAW
                     LAQIAMFLVLGLLVNPRDLLPIAIPALILSAWMIFFARPLSVFAGLLPFRGFNLRERV
                     FISWVGLRGAVPIILAVFPMMAGLENARLFFNVAFFVVLVSLLLQGTSLSWAAKKAKV
                     VVPPVGRPVSRVGLDIHPENPWEQFVYQLSADKWCVGAALRDLHMPKETRIAALFRDN
                     QLLHPTGSTRLREGDVLCVIGRERDLPALGKLFSQSPPVALDQRFFGDFILEASAKYA
                     DVALIYGLEDGREYRDKQQTLGEIVQQLLGAAPVVGDQVEFAGMIWTVAEKEDNEVLK
                     IGVRVAEEEAES"
     gene            complement(11192..12262)
                     /gene="dadX"
                     /locus_tag="J1Y83_002606"
     CDS             complement(11192..12262)
                     /gene="dadX"
                     /locus_tag="J1Y83_002606"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309712.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catabolic alanine racemase DadX"
                     /protein_id="HBA7630746.1"
                     /translation="MTRPIQASLDLQALKQNLSIVRQAAPHARVWSVVKANAYGHGIE
                     RIWSALGATDGFALLNLEEAITLRERGWKGPILMLEGFFHAQDLEIYDQHRLTTCVHS
                     NWQLKALQNARLKAPLDIYLKVNSGMNRLGFQPDRVLTVWQQLRAMANVGEMTLMSHF
                     AEAEHPDGISSAMARIEQAAEGLECRRSLSNSAATLWHPEAHFDWVRPGIILYGASPS
                     GQWRDIANTGLRPVMTLSSEIIGVQTLKAGERVGYGGRYTARDEQRIGIVAAGYADGY
                     PRHAPTGTPVLVDGVRTMTVGTVSMDMLAVDLTPCPQAGIGTPVELWGKEIKIDDVAA
                     AAGTVGYELMCALALRVPVVTV"
     gene            complement(12272..13570)
                     /locus_tag="J1Y83_002607"
     CDS             complement(12272..13570)
                     /locus_tag="J1Y83_002607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-amino acid dehydrogenase"
                     /protein_id="HBA7630747.1"
                     /translation="MRVVILGSGVVGVASAWYLNQAGHEVTVIDREPGAALETSAANA
                     GQISPGYAAPWAAPGVPLKAIKWMFQRHAPLAVRLDGTQFQLKWMWQMLRNCDTSHYM
                     ENKGRMVRLAEYSRDCLKVLRAETNIQYEGRQGGTLQLFRTEQQYENATRDIAVLEDA
                     GVPYQLLESSRLAEVEPALAEVAHKLTGGLQLPNDETGDCQLFTQNLARMAEQAGVKF
                     RFNTPVDQLLCDGEQIYGVKCGDEVIKADAYVMAFGSYSTAMLKGIVDIPVYPLKGYS
                     LTIPIAQEDGAPVSTILDETYKIAITRFDNRIRVGGMAEIVGFNTELLQPRRETLEMV
                     VRDLYPRGGHVEQATFWTGLRPMTPDGTPVVGRTGFKNLWLNTGHGTLGWTMACGSGQ
                     LLSDLLSGRTPAIPYEDLSVARYSRGFTPSRPGHLHGAHS"
     gene            13900..15432
                     /locus_tag="J1Y83_002608"
     CDS             13900..15432
                     /locus_tag="J1Y83_002608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoVR family protein"
                     /protein_id="HBA7630748.1"
                     /translation="MATIDSMNKDTTRLSDGPDWTFDLLDVYLAEIDRVAKLYRLDTY
                     PHQIEVITSEQMMDAYSSVGMPINYPHWSFGKKFIETERLYKHGQQGLAYEIVINSNP
                     CIAYLMEENTITMQALVMAHACYGHNSFFKNNYLFRSWTDASSIVDYLIFARKYITEC
                     EERYGVDEVERLLDSCHALMNYGVDRYKRPQKISLQEEKARQKSREEYLQSQVNMLWR
                     TLPKREEEKTVAEARRYPSEPQENLLYFMEKNAPLLESWQREILRIVRKVSQYFYPQK
                     QTQVMNEGWATFWHYTILNHLYDEGKVTERFMLEFLHSHTNVVFQPPYNSPWYSGINP
                     YALGFAMFQDIKRICQSPTEEDKYWFPDIAGSDWLETLHFAMRDFKDESFISQFLSPK
                     VMRDFRFFTVLDDDRHNYLEISAIHNEEGYREIRNRLSSQYNLSNLEPNIQIWNVDLR
                     GDRSLTLRYIPHNRAPLDRGRKEVLKHVHRLWGFDVMLEQQNEDGSVELLERCPPRMG
                     NL"
     gene            complement(15483..16202)
                     /gene="fadR"
                     /locus_tag="J1Y83_002609"
     CDS             complement(15483..16202)
                     /gene="fadR"
                     /locus_tag="J1Y83_002609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid metabolism transcriptional regulator
                     FadR"
                     /protein_id="HBA7630749.1"
                     /translation="MVIKAQSPAGFAEEYIIESIWNNRFPPGTILPAERELSELIGVT
                     RTTLREVLQRLARDGWLTIQHGKPTKVNNFWETSGLNILETLARLDHESVPQLIDNLL
                     SVRTNISTIFIRTAFRQHPDKAQEVLATANEVADHADAFAELDYNIFRGLAFASGNPI
                     YGLILNGMKGLYTRIGRHYFANPEARSLALGFYHKLSALCSEGAHDQVYETVRRYGHE
                     SGEIWHRMQKNLPGDLAIQGR"
     gene            16424..17965
                     /gene="nhaB"
                     /locus_tag="J1Y83_002610"
     CDS             16424..17965
                     /gene="nhaB"
                     /locus_tag="J1Y83_002610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na(+)/H(+) antiporter NhaB"
                     /protein_id="HBA7630750.1"
                     /translation="MEISWGRALWRNFLGQSPDWYKLALIIFLIVNPLIFLISPFVAG
                     WLLVAEFIFTLAMALKCYPLLPGGLLAIEAVFIGMTSAEHVREEVAANLEVLLLLMFM
                     VAGIYFMKQLLLFIFTRLLLSIRSKMLLSLSFCVAAAFLSAFLDALTVVAVVISVAVG
                     FYGIYHRVASSRTEDTDLQDDSHIDKHYKVVLEQFRGFLRSLMMHAGVGTALGGVMTM
                     VGEPQNLIIAKAAGWHFGDFFLRMSPVTFPVLICGLLTCLLVEKLRWFGYGETLPEKV
                     REVLQQFDDQSRLQRTRQDKIRLIVQAIIGVWLVTALALHLAEVGLIGLSVIILATSL
                     TGVTDEHAIGKAFTESLPFTALLTVFFSVVAVIIDQQLFSPIIQFVLQASEHAQLSLF
                     YIFNGLLSSISDNVFVGTIYINEAKAAMESGAITLKQYELLAVAINTGTNLPSVATPN
                     GQAAFLFLLTSALAPLIRLSYGRMVWMALPYTLVLTLVGLLCVEFTLAPVTEWFMQMG
                     WIATL"
     gene            18111..18641
                     /gene="dsbB"
                     /locus_tag="J1Y83_002611"
     CDS             18111..18641
                     /gene="dsbB"
                     /locus_tag="J1Y83_002611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="disulfide bond formation protein DsbB"
                     /protein_id="HBA7630751.1"
                     /translation="MLRFLNQCSQGRGAWLLMAFTALALELTALWFQHVMLLKPCVLC
                     IYERCALFGVLGAALIGAIAPKTPLRYVAMVIWLYSAFRGVQLTYEHTMLQLYPSPFA
                     TCDFMARFPEWLPLDKWVPQVFVASGDCAERQWEFLGLEMPQWLLGIFIAYLIVAVLV
                     VISQPFKAKKRDLFGR"
     gene            complement(18686..19954)
                     /locus_tag="J1Y83_002612"
     CDS             complement(18686..19954)
                     /locus_tag="J1Y83_002612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HBA7630752.1"
                     /translation="MFALCDVNAFYASCETVFRPDLWGKPVVVLSNNDGCVIARNAEA
                     KALGVKMGDPWFKQKDLFRRCGVVCFSSNYELYADMSNRVMSTLEELSPRVEIYSIDE
                     AFCDLTGVHNCRDLTDFGREIRATVLQRTHLTVGVGIAQTKTLAKLANHAAKKWQRQT
                     GGVVDLSNLERQRKLMSALPVDEVWGIGRRISKKLDAMGIKTVLDLADSDIRFIRKHF
                     NVVLERTVRELRGEPCLQLEEFAPTKQEIICSRSFGERITDYPSMRQAICSYAARAAE
                     KLRSEHQYCRFISTFIKTSPFAFNEPYYGNSASVKLLTPTQDSRDIINAATRSLDAIW
                     QAGHRYQKAGVMLGDFFSQGVAQLNLFDDNAPRPGSEQLMAVMDTLNAKEGRGTLYFA
                     GQGSQQQWQMKRAMLSPCYTTRSSDLLRVK"
     gene            complement(19954..20373)
                     /gene="umuD"
                     /locus_tag="J1Y83_002613"
     CDS             complement(19954..20373)
                     /gene="umuD"
                     /locus_tag="J1Y83_002613"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translesion error-prone DNA polymerase V
                     autoproteolytic subunit"
                     /protein_id="HBA7630753.1"
                     /translation="MLFIKPADLREIVTFPLFSDLVQCGFPSPAADYVEQRIDLNQLL
                     IQHPSATYFVKASGDSMIDGGISDGDLLIVDSAITASHGDIVIAAVDGEFTVKKLQLR
                     PTVQLIPMNSAYSPITISSEDTLDVFGVVIHVVKAMR"
     gene            20570..>20952
                     /locus_tag="J1Y83_002614"
     CDS             20570..>20952
                     /locus_tag="J1Y83_002614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin E"
                     /protein_id="HBA7630754.1"
                     /translation="MEAFAQKGILIKVLDDGIKKLYSVAAGVVEGKLIPELKNKLKSV
                     QSFFTTLSNTVKQANKDIDAAKLKLTTEIAAIGEIKTETETTRFYVDYDDLMLSLLKE
                     AAKKIINTCNEYQKRHGKKTLFEVS"
BASE COUNT         4953 a         5503 c         5297 g         5199 t
ORIGIN      
        1 aggatattac ttatgtcact aaccagacgg aggtttacac agattcttgc gtcgacattg
       61 ttcctgcatc atctgccgtc ctttgcacaa tcagtcaaat tctgggtctc acggacgctt
      121 cccgaagccc aaaacattac acgcatcgtc agcgcaggcg cgcccgccga tttactattg
      181 ctggctgtcg cgccagaaaa aatggtcggt ttttcctctt ttgattttgc ccgtcaggca
      241 ttaattccat tgccagagca cattcgccag ttccccaggc tgggacgact cgccgggcgc
      301 gccagcacac tctcgctgga agggcttatg gcgttacatc ccgatttggt cgttgattgc
      361 ggcaatacgg atgaaacctg gatctcccag gcacggcagg ttagcgaaca gacacaaata
      421 ccctggttat tgcttaacgg gaaactggaa caatcagcag aacagttaac aacgcttggc
      481 aaaacgttag gcgaagagca ccgcgccgca gaacaagcca atctcgccag ccgcttcgtt
      541 ggtgaagctc aggcattcgc cacctcaccc gccgctaatc tcagctttta tgctgcgcgc
      601 ggtcctcgag ggctggaaac gggcttacag ggttcgttgc ataccgaggc ggcggaatta
      661 ttaggtttgc acaacgtcgc gcaaatagcc gatcgccacg gtctgacaca ggtttccatg
      721 gaaaatctcc tgcgctggca gccggatatt attctggttc aggaggccgt tactgcagat
      781 tttattcgtc gtgattctct ctggcagggc gtgaaagcgg ttgcggaaca acgcatctta
      841 tttttaagtg gcctgccctt tggctggctg gatgccccgc cgggaatcaa ccgtcttctg
      901 ggattacgca gacttcacgc ctggctggat cccgccatca atcgccagtt taaaagtgac
      961 atacagcatt acgcccaact gttctggcat tgttcactca gtgacgccga ctatcaaaaa
     1021 ttggtggcga gctaatgaga accgtaaacg gatgtatttt gctgctggca gctatcagta
     1081 tcacttttgc cgccgtaagt ggggcttatc atctcgatat gcagcaactg ctcgcgctca
     1141 ttcttcgtca agaaaatgtt cctgtgcagg aacaaattgt cttctggcaa atccgcgtgc
     1201 cgcgtattct tgctgcgctg tttctgggcg cggcgctggc aggggccgga accacctatc
     1261 agggaatgtt ccgtaatccc ttagtgtcac cggatatttt aggcgtctcc gccggtgccg
     1321 gacttggtgc ctgcgcggca atcctgtggg gattatccat tgtttacatc cagctgtacg
     1381 cattttgcgg cgggctgatg gtggtcgcgg gcgtctggtt gattacgcgt cgggtgaccc
     1441 gccacgatcc gatcttaacc ctggtgctgg taggcattgc gttaggaacg ttgtgcggag
     1501 cggggatatc gctaataaaa acgctggccg atccttacac ccagttaccg tcaattacct
     1561 tctggttgct ggggggctgt ccaccgtgac cttacgcgat ctctgctatg ctgcaccgat
     1621 cattctcata ggctccttgc ccctgttttt tctccgctgg cgcatgaatt tactcacact
     1681 ttccgacgac gaagcccgtt cgctgggact gaacgtgaca cgtctgcgct ttggattaat
     1741 tttctgcgcc acactcatca ccgccagcac ggtagcaata gcgggcataa ttggttgggt
     1801 cggcctggtc gttccgcaca ttgcccgctt gttgaccggt cataaccatc agcagctgtt
     1861 gcccatggca atgtgtactg gcgcgattct gctcctgctt accgatacgc tggcacgcag
     1921 cattggaaca acagaaatcc cacttggcat tctgaccgct tttgttggtg caccgttttt
     1981 cctgtttttg ttgttacgcg gaggtcgcca gtgactttgc ttgccgtccg tcatgcgtct
     2041 ctgggttact cccgccatcc ggtgctacgc gacgtgtcct tcaccctgtc gcaaggaaca
     2101 atttgttgtc tgttaggagc caatggttgc ggcaaaacca cactcatgcg ctccattctt
     2161 ggcgttatac cgttattaaa aggcgagatc ttactggata gcctctctgt gcagacactc
     2221 agccatcgcc agcgcgcgca ggctattgcc tgggtgccgc aggcgcacga cggcattttt
     2281 gcttttagtg tgctggatat ggtgttgatg ggcctggccc ccaccatagg cgctttttcc
     2341 gttcccggta agccagaacg cctgaaagca acggagcaac tggaaaagct gggcattttg
     2401 catctggcgg cacgacgctg gaatacgctt agcggcggtg aacgtcagct ggctttaatc
     2461 gcccgtgcac tcgtccagca accgcgcttg ttattactgg atgagcctgc ctccagcctc
     2521 gatttcggtc atcagatcca acttctggac acgctggcgc agctcaaaaa taacggcatg
     2581 acaatgttaa tgtcgactca tcatccgctc catgccaacg ccattgccga cagcattatc
     2641 caggtagagc cagatggacg agtaacacaa ggcttaccga ccgaacagtt aacaacgaaa
     2701 aaactggctg ccctgtaccg ggtttctgcc gaccagattc accatcacct ctctgcaatt
     2761 aagcactaag gaaaaatcat gctcattgac gatattgatt ttgcagacct ttaccttcag
     2821 caactcaggc tggcccatcg cacagaaaaa acgccggatc actgggatca acgggcggaa
     2881 aaaatggcgg aaaattgcgc ctcgcctacg gacagctacc tgcaacaact gacctcaaaa
     2941 attgacctgc aaggcgcgca aaccttattc gatatgggct gcggaccggg taccgtatcg
     3001 ctggcgctgg cggacaaact cacgacgatt tatggcgttg attacagtca ggggatgctg
     3061 aacgttgccg cccgtcgcgc cgccgcgcta aaggcagaca atgtccactg gatccagcgc
     3121 gcctgggaag aggactggag tgacctgcca cgctgcgata ttgccgtggc ttcccgctcc
     3181 actctggtgg ctgatatgcg ccaggcaatg agcaaactga ataatcaggc acggcttcgc
     3241 gtctatacga ctcatctggt cagcacctcg ttcgtctctc cagccattca gcgggcggct
     3301 ggtcgggaag tgatcgaatt acccaactat atctttgcac tcaacgtcct gtatcagatg
     3361 ggaatttatg ctcacgttga ttttatccgt ggacaaaatt gccaacagga taacagcacc
     3421 tgggaacgtt ttgagcagaa cgtcagctgg agtctgggcg cgcttaacga cgatgagcgt
     3481 gagcggttat atcgctggta tcaacaacaa gatgctcgtg ccctcgcccc tgccagccgc
     3541 gactgggcgc tcatctggtg ggatagcgta ccgcaggaga cactgcgatg attttccttt
     3601 ctcaggcaca aatcgatgcg ttacttctgg aagatatcca gggcggtgac ctgaccacgc
     3661 gagcattaaa tattggacac cagcatggct atatagagtt ttttctccgt cagggcggct
     3721 gcgtcagcgg aatttctgtc gcgtgtaaga tgttaactac gctgggatta actattgatg
     3781 acgcggtcag cgacggttca caagcgaacg cgggtcagcg gctaatccgt gcgcaaggta
     3841 atgccgcagc acttcatcag ggatggaagg cagtccagaa tgtgctggag tggagttgcg
     3901 gtgtttctga ttatctcgct caaatgctgg cgttacttcg tgaacgttac cctgatggca
     3961 atatcgcctg cacccgaaaa gcaattccgg gcacccgctt actggcctcg caggcaattc
     4021 tggctgccgg aggactgatt catcgtgccg gatgtgcgga aaccatatta ttgtttgcca
     4081 accaccgcca ttttcttcat gacaatcagg actggtcagg cgcaatcaat cagttacgtc
     4141 gccacgcacc cgagaagaaa atagttgttg aagccgacac gccgaaagag gcaatcgccg
     4201 cgttacgcgc gcaaccagac gtacttcagc tcgacaaatt tagtccgcaa caggcaactg
     4261 aaattgcgca aatagcaccg tcgctggctc cccactgcac gctggctctg accggcggaa
     4321 ttaatctgac aacactcaaa aattacctcg actgcggcat tcgccttttt attacctccg
     4381 cgccttatta cgcggcacct gctgacatta aagtcagtct gcaacccgca gcctctattt
     4441 aattctttgt taaacggaat ttattctatg agactcaaaa aacgttatct ctgcacagta
     4501 ttatcacttg cctttaccca gcagtccgta gcggctcagg agagtgacac gctgaccgta
     4561 tggtccagtc cggtatcatc gacgacaacc accgttctcg atcaacccac catgaaggcc
     4621 ctggataaac agaatgtcgc tcaggcatta agtgtcgtcc ccggcgtggt gctgcaaaag
     4681 tcaggtagcc gcaacgaaga acaggtcaaa gttcgtggct ttgatagtcg tcaggtgccg
     4741 gtctatttcg acggtgtgcc catttatgtt ccctatgacg gcaacctcga tctggcgcgg
     4801 attctgacca acaatttggg ggcagttgaa gtttccaaag ggtattcgtc gctgcttcag
     4861 gggcctaatc agatgggcgg agccattaat atcaccaccc agaagccaac aaaacctctg
     4921 gaagcaaatc tgggatatcg ccagggatgg agccgtagcc aggacaatgc ctacgatatg
     4981 catgcttcat ttgccgccag cagcgatctg gggtatttgc aagtcagcgg tagccagcta
     5041 aagcaggatt ttctcggcct gccgcatggt gtaaataatg atattgcagg caaacacggc
     5101 aagatgatta attcatcggc tgatgataaa cgcggcattg tgaagctcgg ttttacacca
     5161 cgcgaaaacg atgaatacac attgacttac attaagcagg atggtgaaaa agataacccg
     5221 ccatacagtg gaaatagtgg tcagaaatca cgctactggc agtggccaga gtatgacaaa
     5281 gaaagttttt attatcaggg aacgacccaa ctaaacgatc gttttaccct gaaaagtcgg
     5341 ctgtatcgcg acacctttga aaatacgcta atgatgtaca actcgctggc tgatttgaaa
     5401 aataaaaaag gcagctacag ccattattcc gattacagcg acggtgccgg gttacaactg
     5461 gcagccgatg tgcgtgaaaa cgatctgctg tcgtttgccg ttaactggaa agatgacgta
     5521 caccgggaaa aaggtgcgcc gcacgccgct tacgatcgct atgaagatcg tacctggtcg
     5581 ctcgccagtg aatatcaatg ggctgctgcc gataatgtcg atgtcgtggc tggaatcagc
     5641 tatgactggc gcgatagcgt agaagcgaaa aaacatgaga aagatggcag tatcacccat
     5701 tatgacgaca actatcagtc agcttttaac tggcaggtga tggggaaata ccactttgcc
     5761 aatgaagaca cgctggcgct ttcgtactat gaccgcacac gctttccgac gctgaaagaa
     5821 cgctatacca cgtccaaacc tgcgtataac cagatagcga ttgttaaccc gcagctcaag
     5881 ccggaacgcg cgcgcggggt ggatttaacc tggaatggtg ccttcacgca cgactgggga
     5941 tttgaggtca gcgtttacta taaccgggtg agtgatgcca tcctctcgca caatatcgat
     6001 gccgatacca ttcaaaatca gaacagcggc acggtggatt acagcggtct ggatgccggt
     6061 attaagggga aaatcagcaa tatactggat gtaggattga gctacgccct gatccacgct
     6121 gatgccaaac gcaaagacat cggcaagata accgatctgc caacgcagac aatgaccgca
     6181 tggatgactc tcaaaccgtg ggagccgtta agcgtaacgc tgtcggaaga ggcgcgttcc
     6241 tccagctaca gcaacagtga cggttcacaa aaagccgccg gttttgcggt gacccacatt
     6301 cgagccgatt acaccttagg tcatggcttc agcgttaatg cgtcggtcaa taacctgttt
     6361 gataccaaat atgcctacag tgaagggttt attgaagaag gtcgcaattt ctgggcaggt
     6421 atcgagtata cgttctgatg tgaaagcgga tagcggcatc tgtatcgcta tccgccttat
     6481 gaaaatctta ttctcttcgc aggaggcgga atacgcccag caccaggata gcgcccacca
     6541 ccgccaccag gaagctgtgc aaattaaaac cgctgatgga gccgccaatg ccaaacatgg
     6601 tcgccagcca gccgccgacc accgcaccga ctatcccaag aatacaggtc aggaaaaatc
     6661 caccaccatc acgccccggc atgattagct tggcgataat accggctatc aggccaaaaa
     6721 taatccaggc aataattccc atgttcaggc cctcgtttcg atgaatcgga cgcaagaaat
     6781 gcttacgtca acgctctaag tataggcaag ggcgggatcg atgctgggcg tatgtcggct
     6841 gatttaagaa caacatgtaa aaaatattcg cgcatttgta ttaagttttg ttaactgcga
     6901 ccgataaacc aaagacagtt tgtcagtcag gagtttttcc gcgtgagtca ttaccatgag
     6961 cagttcctga aacaaaatcc gttagccgtc ctgggcgtgt tacgcgattt gaacaaagcc
     7021 gcaattcctt tgcgtatcag ctggaatggc gggcagctga tcagcaaaat actggcaata
     7081 actccggata aactggtgct ggatttcggc agccaggccg aagacaacat cgccgtgcta
     7141 aaggcgcagc acattaccat taccgccgaa actcagggtg cgaaagtcga gtttactgtt
     7201 gaacaactac agcagagtga atacttgctg cttccggcat ttattaccgt accgcctccc
     7261 accttatggt ttgtacaacg acgccgatat ttccgcatca ccgccccact ccatccgcct
     7321 tatttttgcc agaccaaact ggcggataac agtacgttac gtttccgcct gtatgatttg
     7381 tcgttaggcg gcatgggcgc attactggaa acagcaaagc ctgccgaatt acacgaaggc
     7441 atgcgcttcg ctcagattga agtcaacatg gggcaatggg gtgtttttca ctttgacgcc
     7501 cagttaatct ccatcagcga gcgcaaagtg attgatggca aaaatgaaac catcaccact
     7561 ccccgtctga gcttccgttt tcttaacgtc agcccgacgg tggagcggca attacagcgg
     7621 attattttct ctctcgagcg agaagcccgg aaaaaagcgg acaatgtgcg cgactgattt
     7681 acatcgcgtc cagtgcctgc tcaagtttgt agatatagcg cggagcctgc ggcgcaggat
     7741 gatttcgcgc tacgtgatcg aggaactcat cagcatccag atcgttgatt ttgctgatcg
     7801 cctttttccg atctgacgag aaagtccgta gcagcgcacc tgccccgtta gcgtatgaca
     7861 ccaccagcgc atattgcagt accttcggat cttcaatgcc tgccagcggg ccggtttcca
     7921 gaatattcag gtaagccgcc cccattgaaa tattacgctc cggatttttc agctcgctgg
     7981 tcgtcggctc accgctccag cccatacgac gatatacatc acgtccggag gttgaagctt
     8041 ttatctgcat caaaccaatg gcattcgatt tactcaccgc gttcggatta ccacccgatt
     8101 cgatagcgat aatcgccgtg atcaattgtg gatcgacgcc ccaggctgca ccggctttct
     8161 ggcttattgg catccactgc atcgcacgtt gcaccggaac tttcgcgttc cacggcgggt
     8221 tcgtatagtc atgctttgat gaacaacccg ctaataacac aatcaaaaag gcaaaccatc
     8281 tcaatttcac ttcttctatc cttatagcct ggaatcgccg ttgaggtgac aacggcacac
     8341 ctaagcggca tgatatacat ttcgtttctg ttgtcagcaa ggaattgcca tgtctctgtt
     8401 tcacttaatt gccccatcgg gttactgcat taaacagcac gccgcgctgc gtggtatcca
     8461 gcgccttacc gacgcggggc atcaggtcaa taacgtcgag gttattgccc gtcgttgtga
     8521 gcgttttgcg ggtacggaaa cggagcgtct ggaagatctc aattctcttg ccagactgac
     8581 tatccccaac accatcgtgc tgtctgtacg cggcggttac ggtgccagtc gtttactggc
     8641 agatattgac tggcaggcac tggtggctcg ccaacaacat gacccgttac tcatttgcgg
     8701 acatagcgat tttaccgcca ttcagtgcgg tcttctggcg caaggcaatg tcatcacctt
     8761 tagcggcccc atgctggtag caaactttgg cgcggatgaa ctaaatgcct ttaccgagca
     8821 ccacttctgg ctggcgttac gcaataaaac gttcaccatt gaatggcaag gggaaggccc
     8881 gacatgtcag accgaaggca cgctgtgggg aggcaatctt gcgatgctga tttcactgat
     8941 tggtacaccg tggatgccaa aaattgagaa cggtattctg gtgctggaag atattaacga
     9001 gcaccctttc cgcgtcgaac gtatgctgtt gcagctctat catgctggaa ttttgccacg
     9061 tcagaaggcg attattctcg gtagctttag cggcagcacg cctaatgatt atgacgcagg
     9121 ttacaacctg gagtcagtgt acgcgttttt gcgttcccgc ctgtcgattc cactcattac
     9181 cggcctcgat tttggtcatg aacaacgcac ggttacgctg ccgttgggcg cacatgccat
     9241 tctgaataac acccaggaag gcactcagtt gacgattagc ggtcatcctg ttcttaaaat
     9301 gtaaaaaaat cacgcttcag ggctaagtaa aacgctgtct ctgccgctaa tatggtaggg
     9361 tttatataag agacagcgta atcaggagta accgaccgtt ggatgccaca acaataatta
     9421 gccttttcat cttaggatcc atccttgtca ccagcagtat tttacttagt tcattttctt
     9481 cccgtcttgg cattcccatt ctggttattt ttctggcgat cggcatgctg gcgggggttg
     9541 atggcgtcgg cggtatcccg tttgataatt atcccttcgc ctacatggta agtaacctgg
     9601 cgctggcgat tattttgctc gacggcggga tgcgcactca ggccagttcc tttcgtgtgg
     9661 cgttaggacc ggcactgtcg ctggcgacgc tgggcgtgct tatcacctct ggtttaaccg
     9721 gcatgatggc ggcgtggctg tttaatcttg atttgattga aggattatta atcggcgcta
     9781 tcgtcggctc caccgatgct gcagcggtct tttctctgct gggtggtaag gggcttaacg
     9841 aacgtgttgg ctcgacgctg gaaattgaat ccggcagtaa tgatccaatg gcggtctttc
     9901 tgacgattac cctgattgcg atgatccagc agcatgaaag cagtgtcagc tggatgttcg
     9961 tggtcgatat tctgcagcaa tttggcctcg ggattgtcat cgggcttggc ggtggttatt
    10021 tactgctgca aatgattaat cgcatcgccc tgcccgccgg attatatcca ttgctggcat
    10081 taagcggcgg tattttaatt tttgctttaa ccacggcgct ggaaggcagc ggtattctgg
    10141 ctgtttatct gtgcggtttt ctgctgggta atcggccgat tcgcaaccgc tacggcatcc
    10201 tgcaaaattt cgacggcctc gcctggctgg cgcaaatcgc catgttcctg gtgctggggc
    10261 tattggttaa cccaagagat ctgctgccta ttgccattcc ggcgctcatt ttgtccgcat
    10321 ggatgatttt cttcgcccgt cctctttcgg tatttgccgg attgctaccg ttccgtggct
    10381 tcaatctgcg tgagcgcgtg tttatcagct gggtaggatt acgcggcgcg gtgccgatta
    10441 ttctggctgt gtttccgatg atggcggggc tggagaatgc gcgactgttc tttaatgtcg
    10501 ccttctttgt ggtcctggtt tcactgctat tgcagggaac atcactctcg tgggcggcga
    10561 aaaaagccaa agtggtcgtt ccgccagtgg gacgtccggt gtcacgcgtt ggcctggata
    10621 ttcatccgga aaatccgtgg gagcagtttg tttatcaatt gagtgccgat aaatggtgcg
    10681 tgggcgcggc actgcgtgat ttgcatatgc caaaagagac gcgtattgcg gcactgtttc
    10741 gtgataacca gctgcttcat cctaccggca gcacccgact gcgcgaaggc gatgtattgt
    10801 gtgtaattgg tcgggaacgc gatctcccgg cgctcggtaa actgttcagc cagtcgccgc
    10861 cggtcgcgct ggatcaacgc ttctttggtg acttcattct cgaagccagc gccaaatatg
    10921 ctgatgtggc gctgatatat ggcctggaag acgggcgaga atatcgcgat aagcagcaaa
    10981 cgctgggtga aatcgtccag cagttgttag gcgcagcacc ggttgtcggt gaccaggtag
    11041 agtttgccgg gatgatctgg acggtggccg agaaagaaga taatgaagtg ttgaagattg
    11101 gtgttcgggt agcggaggaa gaagccgaat cttaagttta atgtgttttg taacgctgga
    11161 tacgacgctg gtcgcatcca gcgataacaa gttacactgt cacaaccggg acacgtaacg
    11221 ccagcgcgca catcaactca tagcccaccg ttccggcagc ggcagcgaca tcatcaattt
    11281 tgatctcctt gccccacagc tcaaccggcg taccaatccc cgcctgcggg caaggcgtta
    11341 aatcaaccgc cagcatatcc atcgagacgg tccccaccgt catggtgcgc acgccgtcca
    11401 ctaaaacagg agtaccggta ggcgcatggc gcggataacc gtcggcgtat cctgcggcga
    11461 caatgccgat tcgctgttca tcgcgcgcag tatagcgacc gccgtagccc acacgctcgc
    11521 ccgcttttag cgtctggaca ccaataatct cactgcttag cgtcatcacc ggacgtaatc
    11581 cggtattggc gatatcacgc cactgaccgg acggcgaagc gccatacaaa ataatgccag
    11641 gccgaaccca gtcaaaatgc gcttccgggt gccacagagt cgccgccgaa ttggacaacg
    11701 aacgccgaca ctccagccct tccgccgcct gctcaatacg cgccatcgcg ctggaaattc
    11761 catcaggatg ttccgcctcg gcaaaatgcg acatcagggt catttcgcca acattcgcca
    11821 tcgcccgcaa ctgctgccag acggtaagca cgcgatcggg ctggaagccc aaccgattca
    11881 tcccactgtt cactttaaga taaatatcca acggggcttt tagccgtgca ttttgcagtg
    11941 ctttgagctg ccagttgctg tgtacacagg tggtcaggcg gtgctggtca taaatctcca
    12001 gatcctgagc atggaaaaat ccttccagca tcaggatcgg tcctttccag ccgcgctcac
    12061 gtaacgttat tgcctcttcc aggttaagta atgcaaagcc atcggtggcc ccgagcgcgc
    12121 tccagatacg ctcaataccg tgcccgtaag cgttcgcttt taccaccgac cagacgcgcg
    12181 cgtgcggcgc ggcctggcgg acaatggaca gattctgttt taatgcctgc agatcgaggc
    12241 tggcctgtat cggacgggtc atctcgtttc cttagctgtg tgcgccatgt aaatggcccg
    12301 ggcgtgatgg cgtaaatcca cggctgtagc gcgctacgct tagatcctca tatgggatcg
    12361 ctggcgtgcg accagagagc agatcgctta acaactgacc ggaaccgcaa gccatcgtcc
    12421 agccgagcgt gccgtgaccg gtattcagcc acagattttt aaagcctgta cgcccgacaa
    12481 ccggcgtgcc gtctggcgtc atcgggcgca gacccgtcca gaaagtcgcc tgctcgacat
    12541 gaccgccgcg tggatagaga tcgcgaacca ccatttccag cgtttcacga cgcggttgca
    12601 acagctcggt gttaaaacca acaatttccg ccattccgcc aacacgaatg cggttatcga
    12661 aacgggtaat ggcgattttg taggtttcat caagaatggt ggataccggc gcaccatctt
    12721 cctgcgcaat gggaatggtc agcgagtagc ctttcaaagg atacaccgga atatcaacaa
    12781 tgcctttgag catcgccgtc gagtaagaac caaacgccat cacatacgca tcggccttaa
    12841 tcacttcatc accacacttc acgccgtaaa tttgctcgcc gtcgcaaagc agttggtcaa
    12901 cgggcgtatt aaagcggaat ttaacccccg cctgctccgc catccgcgcc agattctggg
    12961 taaacagctg gcagtctccg gtttcatcat tgggcagttg caggccgccc gtcagtttgt
    13021 gcgccacttc tgccaacgcg ggctccactt ccgccaggcg gctggattcc agcagctgat
    13081 acggtacgcc agcatcttcc agcacggcaa tatcgcgggt cgcgttttca tactgttgtt
    13141 cggtacggaa cagttgcagc gtcccaccct gacgcccttc atactgaata ttggtttcgg
    13201 cgcgcaatac tttcaggcaa tcacggctgt attccgccag acgtaccatc cgccctttgt
    13261 tttccatgta gtggctggtg tcgcagttac gtaacatttg ccacatccat ttcaactgga
    13321 actgcgtacc gtcgagacga accgccagcg gcgcatggcg ctggaacatc catttaatcg
    13381 cttttaaagg cacacctggt gccgcccacg gtgcagcata tccgggggag atttgcccgg
    13441 cattcgcggc actggtttcc agggcggctc ccggctcccg atcaatgacg gtgacctcat
    13501 gtcctgcctg atttaagtac caggcgctgg caacgcctac cacaccactt cccagtatga
    13561 caactcgcat agccactccg ataacggtag atacagtaaa agaataataa tctaattaca
    13621 tcttgataac ccagttgaaa atattattca acatacagct tttttatggt gagctggctc
    13681 acatctccct gactctgcgg caatatgacg tctttagtat ctcctgctga acgccctttt
    13741 tatccagcat aaaattccgt tcagaagcgg attagtggca ctctgataat aagaaatcgc
    13801 aaagaaaaaa attctccggc gacgcggttt ttaacaggtg ttctatgctt gaaatgaggt
    13861 gctccacaca gagagcgccg acaacaatga ggtgcgcgta tggcgacgat cgattctatg
    13921 aataaggaca ccacacgttt gagcgatgga cccgactgga cgttcgacct gctggatgtt
    13981 tatctggcag agatagaccg ggtggcgaaa ctctaccggc tggataccta cccgcaccag
    14041 attgaagtga taacctcaga acagatgatg gatgcctact ccagcgtcgg catgccaatt
    14101 aactatccgc actggtcatt cggtaaaaag tttatcgaga ctgaacggct gtataagcac
    14161 ggtcagcaag gactggccta tgaaatcgtc attaactcta acccgtgtat cgcttacctg
    14221 atggaagaga acaccattac catgcaagcg ctggtgatgg ctcatgcctg ctatgggcat
    14281 aactctttct tcaaaaacaa ttacttattc cgtagctgga ccgacgccag ttcgattgtc
    14341 gattatctga tttttgcccg taaatatatt accgagtgcg aagagcgtta tggcgttgat
    14401 gaagtagaac ggcttctgga ctcgtgccac gcgctgatga actacggcgt ggaccggtac
    14461 aaacgcccgc aaaaaatctc gctgcaagaa gagaaagccc ggcagaagag tcgcgaagag
    14521 tatctgcaaa gtcaggtcaa tatgctctgg cgtaccctgc cgaagcgcga ggaagagaaa
    14581 acggttgctg aagcgcgccg ctatccgtcc gaaccacaag aaaacctgct ctattttatg
    14641 gagaaaaatg cgccactgct ggaatcatgg cagcgtgaaa tcctgcgtat tgtgcgtaag
    14701 gtgagccagt atttttatcc gcaaaaacag actcaggtga tgaacgaagg ctgggcgacc
    14761 ttctggcact acaccatcct taaccatctg tatgatgaag ggaaagtaac ggaacgtttt
    14821 atgctggagt ttttgcacag ccacaccaat gtggtcttcc agccgcccta taacagcccg
    14881 tggtacagcg gcatcaatcc gtatgccctc gggtttgcca tgttccagga tattaaacgg
    14941 atttgtcagt cgccaacgga agaagacaaa tactggttcc cggatatcgc cggttccgac
    15001 tggctggaaa cgctgcattt cgcgatgcgt gatttcaaag atgagagttt tatcagccag
    15061 ttcctgtcac cgaaagtgat gcgtgatttc cgcttcttca ccgtgctgga tgatgatcgg
    15121 cataattatc tggagatttc cgctattcat aatgaagaag gttatcggga aatccgtaac
    15181 cggttatcat cgcaatataa cttaagtaat ctggagccga atattcagat ctggaacgtg
    15241 gatttgcgcg gcgaccgttc gctgacgctg cgttacattc cacataatcg cgcaccgctg
    15301 gatcgggggc gcaaagaagt gctgaagcat gtgcatcgcc tgtggggatt tgatgtgatg
    15361 ctggaacagc aaaacgaaga cggcagcgtc gagttgctgg aacgttgccc gccgagaatg
    15421 ggaaatctgt aaaaacaatg aaaacccctc gtttgagggg tttgcttttt aaacggaagg
    15481 gattatcgcc cctgaatggc taaatcaccc ggcagatttt tctgcatccg gtgccaaatc
    15541 tcgccactct catgcccata gcgacgcact gtttcatata cctgatcgtg tgcgccttca
    15601 ctgcacaacg ccgacagttt gtggtagaag cccagcgcca gactgcgcgc ttccggattg
    15661 gcgaaatagt gacgaccaat gcgcgtatac agccctttca tcccgttaag gatcagaccg
    15721 taaatcgggt tgccggaagc aaacgccagg ccgcggaaaa tgttgtaatc cagctcggca
    15781 aaggcgtcgg cgtgatcggc cacttcatta gcggttgcca gcacttcctg cgctttatcg
    15841 ggatgttgac gaaacgcggt gcgaataaaa atagttgaaa tattggtacg caccgacagc
    15901 aaattatcaa taagttgcgg cacactttcg tggtccagtc gcgccagtgt ttcaaggata
    15961 tttaaaccgg aggtttccca gaaattattc accttcgtcg gcttgccgtg ttgaatggtc
    16021 aaccagccat ctcgtgccag acgttgtaac acttcacgta acgtagtacg cgttacgcca
    16081 attaattctg aaagttcacg ttctgcgggc aaaatagtcc cgggagggaa gcggttattc
    16141 cagatacttt caataatgta ctcttccgcg aaacccgccg ggctttgcgc cttaatgacc
    16201 atagtgagat ttccataaca cagcaaaaca aagttggact catcatacca gagggctaac
    16261 tacgctgata gaagacaaaa agaagaaaaa agggatcaag gcttcatttt ctgcatctct
    16321 ttccccttga ctatcaagac ttgcctcact cctgtctgac cgttaagctt tgcgtttaaa
    16381 cataaaaaca tcattgatta tttctaaggt aagggaaacc atcatggaga tctcctgggg
    16441 ccgcgcacta tggcgcaatt ttttgggcca gtcccccgac tggtacaaac tcgccctcat
    16501 cattttctta atcgtaaatc cgttaatttt cctcatcagc cctttcgtcg ctggctggtt
    16561 gctggtagcg gaatttattt tcactctggc gatggccctg aaatgctacc cgctgctccc
    16621 cggtggtctg ttggctatcg aagcggtatt catcggcatg accagcgcgg aacatgttcg
    16681 tgaagaagtg gcggcaaatc ttgaagtctt gctgttactg atgtttatgg tggcgggtat
    16741 ctattttatg aaacagctgt tgttgttcat atttacccgt ttgctgttaa gtattcgctc
    16801 caaaatgctg ctgtcgctct ctttttgcgt ggcggcggcg ttcctctccg cgttcctcga
    16861 tgccttaacc gtcgtggcgg tggtgatcag cgttgcagtc ggtttttatg gtatttatca
    16921 ccgcgtagcc tcttcccgta ctgaagatac cgacctgcaa gacgatagtc atatcgacaa
    16981 gcattacaaa gtggttctgg aacagttccg tggctttctg cgtagtctga tgatgcatgc
    17041 cggtgtcggc accgcattag gcggcgtgat gaccatggtg ggcgaaccac agaacctgat
    17101 catcgctaaa gcggctggct ggcattttgg cgatttcttc ctgcgcatgt cgccggtgac
    17161 cttcccggtt ctgatttgtg gcctgttaac ctgcctgctg gtagagaagc tgcgttggtt
    17221 tggctacggt gaaacgctgc cggagaaagt ccgcgaagtg ctgcaacagt ttgacgatca
    17281 aagccgtctc cagcgtacac gtcaggacaa aattcgtctg attgtgcagg cgattatcgg
    17341 cgtctggctg gtgactgcgc tggcactgca tctggcggaa gttggtttga ttggtttgtc
    17401 agtcattatt ctggcgacat cattgaccgg tgttaccgat gaacatgcta tcggtaaagc
    17461 cttcaccgaa tctctgccat tcaccgcact gttgacggtg tttttctcag tagtcgcggt
    17521 gattatcgac caacaactgt tttcgccgat tattcagttt gtgttgcagg catctgaaca
    17581 tgctcagctg tcgttgttct atattttcaa cggtctgctg tcatccattt cggataacgt
    17641 cttcgtgggg acgatttata tcaacgaagc gaaagcggca atggaaagtg gcgctatcac
    17701 gttgaagcaa tatgagctgc tggcggtcgc cattaatacc ggtaccaatc tgccctccgt
    17761 cgctacgccg aacggtcagg ctgcgttcct gttcctgctg acctctgcac tcgcgccatt
    17821 gattcgtctc tcttatggcc gcatggtgtg gatggccctg ccttacaccc tcgtcctgac
    17881 actcgtcggg ctgctctgcg tcgagtttac gcttgctcct gtaaccgaat ggtttatgca
    17941 aatgggctgg atagcaacgc tttgataaca acttaccggg catataaatg cccggtttgc
    18001 cttttcgccc gataattgtc caattgcgtc ctatttttgc tgcctcctgg tggcgcagcg
    18061 aatgaattgg tttaaactgc gcactctatg catattgcag ggaaatgatt atgttgcgat
    18121 ttttgaacca atgttcacaa ggccggggcg catggctgtt gatggcgttt actgctctgg
    18181 cactggaact gacggcgctg tggttccagc atgtgatgtt actgaaacct tgcgtgctct
    18241 gtatttatga acgctgcgcg ttattcggcg ttctgggtgc tgcgctgatt ggcgcgatcg
    18301 ccccgaaaac tccgctgcgt tatgtagcga tggttatctg gttgtatagt gcgttccgcg
    18361 gtgtgcagtt aacttacgag cacaccatgc ttcagctcta tccttcgccg tttgcgacct
    18421 gtgattttat ggctcgtttc ccggaatggc tgccgctgga taaatgggtg ccgcaagtgt
    18481 ttgtcgcctc tggcgattgc gccgagcgtc agtgggaatt tttaggtctg gaaatgccgc
    18541 agtggctgct cggtattttt atcgcttacc tgattgtcgc ggtgctggtg gtgatttccc
    18601 agccgtttaa agcgaaaaaa cgtgacctgt tcggtcgcta atccattcgg cgctcctgcg
    18661 ggagcgcttt ttcctgccgc tatgtttatt tgacccgcag taaatcagaa cttcgcgttg
    18721 tataacatgg tgaaagcatg gcacgcttca tctgccattg ttgctggctc ccctgcccgg
    18781 caaaatagag tgttcctctg ccctctttag cattcagcgt atccattacc gccatcaatt
    18841 gctcactccc ggggcgcggt gcgttgtcat cgaataaatt gagctgcgcg actccctgac
    18901 tgaagaaatc ccccagcatc acgcccgctt tttggtagcg atggcccgct tgccagatag
    18961 catccagaga tcgcgtagcg gcgttaatga tatccctgct gtcctgagtg ggcgtcagca
    19021 gttttaccga tgcgctattg ccgtaataag gttcattgaa cgcaaatggt gacgtcttaa
    19081 taaacgtgga gataaaccga caatattgat gctcgctgcg aagtttttcc gccgcccggg
    19141 cagcgtaact acaaatggcc tgccgcatcg acggataatc cgtgatgcgt tcaccaaacg
    19201 agcgggaaca gataatttcc tgcttcgtcg gtgcaaactc ttccagttgc aaacagggtt
    19261 caccgcgcag ttcacgcacc gttctttcga gcacgacatt aaaatgttta cggataaacc
    19321 ggatatctga atccgccaaa tcgagaacag ttttgatccc catcgcgtcc agttttttgc
    19381 tgatccgccg tccaatcccc cagacttcat ccacggggag agcagacatt aatttacgct
    19441 ggcgttccag atttgataaa tccaccaccc cgcccgtctg ccgctgccat ttttttgccg
    19501 catgattggc aagcttagcc agcgttttgg tctgggcgat ccccacacca acagtaagat
    19561 gggtacgttg tagcaccgtt gcgcgaattt ctctgccaaa atcagtcaga tcgcgacaat
    19621 tatgcacacc tgtcagatcg cagaatgcct catcaatact gtaaatctcg acgcggggcg
    19681 atagttcttc cagcgtcgac atcacccgat tactcatgtc tgcgtaaagc tcataattgc
    19741 tgctaaagca aaccacgcca cagcgacgaa acagatcttt ttgtttgaac cagggatcgc
    19801 ccattttaac gcccagcgcc tttgcctcag cgtttcgggc gataacgcaa ccgtcattat
    19861 tcgatagcac aaccaccggt ttaccccata aatcagggcg aaacaccgtc tcacagctgg
    19921 cataaaacgc gtttacatca cagagggcaa acatcagcgc atcgccttaa cgacgtggat
    19981 caccacacca aagacatcca gcgtatcttc gctactgatg gtaatgggcg agtacgcact
    20041 gttcatagga ataagctgta ccgtcgggcg tagttgcaat tttttcaccg taaactcacc
    20101 gtcaacagca gcgatgacaa tatcaccatg gctggcggta atagcgctat cgacaatcag
    20161 taaatcaccg tcactaatcc caccatcaat catagaatca ccacttgctt tgacgaaata
    20221 agtcgcgctg ggatgctgga tcaacagttg attcagatcg atgcgctgtt caacgtaatc
    20281 tgcggccggt gaaggaaagc cacactgaac aagatcgcta aatagcggaa aagtcacaat
    20341 ttcgcggaga tccgcaggct tgataaacaa cataataatc tgcctgaagc tatactgttt
    20401 ttatatacag tagtctgttc ttgtcagcag atcaatactg attcaggcta tcaatatttg
    20461 tcgctgcata gactgctgat ttttcgttct cttatcctgt gctcacgcgg ccttcttgcg
    20521 acagcgtcca tccagcagaa aggaaaaata tcacctggtt taaaatagaa tcgaagcatt
    20581 cgcccagaaa ggcattctca ttaaggtact ggatgacggc atcaagaaac tgtattctgt
    20641 tgcagcgggc gtagttgaag ggaaactgat tccagaattg aagaacaagt taaaatctgt
    20701 gcagagtttc tttaccaccc tgtctaacac ggttaaacaa gcgaataaag atatcgatgc
    20761 cgccaaattg aaattaacca ccgaaatagc cgccatcggg gagataaaaa cggaaactga
    20821 aacaaccaga ttctacgttg attatgatga tttaatgctt tctttactaa aagaagcggc
    20881 caaaaaaata attaacacct gtaatgagta tcagaaaaga cacggtaaga agacactctt
    20941 tgaggtatct ga
//
LOCUS       DADSYM010000082        20897 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2415
            SAMN08039579-rid11723173.denovo.082, whole genome shotgun sequence.
ACCESSION   DADSYM010000082 DADSYM010000000
VERSION     DADSYM010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039579
            Sequence Read Archive: SRR6362533
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 20897)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20897)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980162.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 03:39:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,484
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 4,347
            CDSs (with protein)               :: 4,347
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 43 of 85
            Pseudo Genes (incomplete)         :: 37 of 85
            Pseudo Genes (internal stop)      :: 19 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20897
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039579-rid11723173.denovo.082"
                     /strain="LGE2415"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8302 N 95.9628 W"
                     /collection_date="2015-08-24"
     gene            102..704
                     /locus_tag="J1Y83_002615"
     CDS             102..704
                     /locus_tag="J1Y83_002615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HBA7630755.1"
                     /translation="MLAFCRSSLKSKKYFIILLALAAIAGLGTHAAWSSNGLPRIDNK
                     TLARLAQQHPVVVLFRHAERCDRSTNQCLSDKTGITVKGTQDARELGNAFSADIPDFD
                     LYSSNTVRTIQSATWFSAGKKLTVDKRFLQCGNEIYSAIKDLQRKAPDKNIVIFTHNH
                     CLTYIAKDKRDATFKPDYLDGLVMHVEKGKVYLDGEFVNH"
     gene            complement(743..1168)
                     /gene="nudI"
                     /locus_tag="J1Y83_002616"
     CDS             complement(743..1168)
                     /gene="nudI"
                     /locus_tag="J1Y83_002616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphatase NudI"
                     /protein_id="HBA7630756.1"
                     /translation="MRQRTIVCPLIQNDGAYLLCKMADDRGVFPGQWALSGGGVEPGE
                     RIEEALRREIREELGEQLLLTEITPWTFSDDIRTKTYADGRKEEIYMIYLIFDCVSAN
                     RDVKINEEFQDYAWVKPEDLVHYDLNVATRKTLRLKGLL"
     gene            1447..1989
                     /locus_tag="J1Y83_002617"
     CDS             1447..1989
                     /locus_tag="J1Y83_002617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA7630757.1"
                     /translation="MKKIALAGLAGMLLVSASVNAMSISGQAGKEYTNIGVGFGTEST
                     GLALSGNWTHNDDDGDAAGVGLGLNLPLGPLMATVGGKGVYTNPNDGDEGYAAAVGGG
                     LQWQIGDSFRLFGEYYYSPDSLSSGIKSYEEANAGARYTIMRPVSIEAGYRYLNLAGK
                     NGNRDNAIADGPYVGVNASF"
     gene            2089..3291
                     /locus_tag="J1Y83_002618"
     CDS             2089..3291
                     /locus_tag="J1Y83_002618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide mononucleotide deamidase-related
                     protein YfaY"
                     /protein_id="HBA7630758.1"
                     /translation="MLKVEMLSTGDEVLHGQIVDTNAAWLADFFFHQGLPLSRRNTVG
                     DNLDDLVTILRERSQHADVLIVNGGLGPTSDDLSALAAATAKGEGLVLHEAWLKEMER
                     YFHERGRVMAPSNRKQAELPASAEFINNPVGTACGFAIQLNRCLMFFTPGVPSEFKVM
                     VEHEILPRLRERFSLPQPPVCLRLTTFGRSESDLAQSLDTLQLPPGVTMGYRSSMPII
                     ELKLTGPASEEQAMEKLWLDVKRVAGQSVIFEGTEGLPAQISRELQSRQFSLTLSEQF
                     TGGLLALQLSRADAPLLACEVVPSQEETLAQTAHWITERRANHFAGLALAVSGLEDEH
                     LNFALATPDGTFALRVHFSATRYSLAIRQEVCAMMALNMLRRWLNGQDIASEHGWIEV
                     VESMTLSV"
     gene            3511..4293
                     /locus_tag="J1Y83_002619"
     CDS             3511..4293
                     /locus_tag="J1Y83_002619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HBA7630759.1"
                     /translation="MLESSKVPALTRAIDILNLIARIGPCSAATIIDTLGIPKSTAYL
                     LLNELRRQRFLSLDHQENFCLWTRLVELSGHALSKMDLRELARPRLTQLMDTTGLLCH
                     LGIIDNGSAYYILKVESSATISVRSHEGKSLSLYRSGIGKCLLAWQPEAVQKSIIEEL
                     VWEQMTPTTITHPQRLYEELARIRRQGWSYDNGEDYADVRCVAAPVFNANNELTAAIS
                     VVGTRLQINEEYRDYLAGKAIACARDISRLLGWKSPFDLQAS"
     gene            4308..5513
                     /gene="rhmD"
                     /locus_tag="J1Y83_002620"
     CDS             4308..5513
                     /gene="rhmD"
                     /locus_tag="J1Y83_002620"
                     /EC_number="4.2.1.90"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311162.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnonate dehydratase"
                     /protein_id="HBA7630760.1"
                     /translation="MTLPKIKQVRAWFTGGATAEKGAGGGDYHDQGANHWIDDHIATP
                     MSKYRDYEQSRQSFGINVLGTLIVEVEAENGQTGFAVSTAGEMGCFIVEKHLNRFIEG
                     KCVSDIKLIHDQMLNATLYYSGSGGLVMNTISCVDLALWDLFGKVVGLPVYKLLGGAV
                     RDEIQFYATGARPDLAKEMGFIGGKMPTHWGPHDGDAGIRKDAAMVADMREKCGEDFW
                     LMLDCWMSQDVNYATKLAHACAPYNLKWIEECLPPQQYEGYRELKRNAPAGMMVTSGE
                     HHCTLQSFRTLSETGIDIMQPDVGWCGGLTTLVEIAAIAKSRGQLVVPHGSSVYSHHA
                     VITFTNTPFSEFLMTSPDCSTMRPQFDPILLNEPVPVNGRIHKSVLDKPGFGVELNRD
                     CNLKRPYSH"
     gene            5570..6859
                     /locus_tag="J1Y83_002621"
     CDS             5570..6859
                     /locus_tag="J1Y83_002621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416749.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBA7630761.1"
                     /translation="MSTALLDAVVKKNRARLIPFMLALYVLAFLDRSNIGFAKQTYQI
                     DTGLSNEAYALGAGIFFVVYAFLGVPANLLMRKLGARTWIGTTTLLWGFLSAAMAWAD
                     TEAKFLIVRTLLGAAEAGFFPGMIYLTSQWFPQRNRASIMGLFYMGAPLALTLGSPLS
                     GALLEMHGFMGHPGWFWMFVIEGLLAVGAGVFTFFWLDDSPEQARFLSKQEKTLLINQ
                     LASEEQQKVTSRLSDALRNGRVWQLAIIYLTIQVAVYGLIFFLPTQVAALLGTKVGFT
                     ASVVTAIPWVAALFGTWLIPRYSDKTGERRNVAALTLLAAGIGIGLSGLLSPVLAIVA
                     LCVAAIGFIAVQPVFWTMPTQLLSGTALAAGIGFVNLFGAVGGFIAPILRVKAETLFS
                     SDAAGLLTLAAVAVIGSLIIFTLRVNRTVAQTDVAHH"
     gene            6877..7680
                     /gene="yfaU"
                     /locus_tag="J1Y83_002622"
     CDS             6877..7680
                     /gene="yfaU"
                     /locus_tag="J1Y83_002622"
                     /EC_number="4.1.2.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-3-deoxy-L-rhamnonate aldolase"
                     /protein_id="HBA7630762.1"
                     /translation="MNALLSNSFKERLRKGEVQIGLWLSSTTAYMAEIAATSGYDWLL
                     IDGEHAPNTIQDLYHQLQAVASYTSHPVIRPVEGSKPLIKQVLDIGAQTLLIPMVDTA
                     DQARQVVSATRYPPYGERGVGASVARAARWGRIENYMAQVNDSLCLLVQVESKTALDN
                     LDEILGVEGIDGVFIGPADLSASLGYPDNAGHPEVQRIIETSIRRIRAAGKAAGFLAV
                     APDMAQQCLAWGANFVAVGVDTMLYSDALDQRLAMFKSGKNGPRVKGSY"
     gene            complement(7721..8635)
                     /locus_tag="J1Y83_002623"
     CDS             complement(7721..8635)
                     /locus_tag="J1Y83_002623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001613492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISNCY family transposase"
                     /protein_id="HBA7630763.1"
                     /translation="MTESTTSSPHDAVFKTFMFSPDTARDFLEIHLPEPLRKLCNLQT
                     LRLEPTSFIEKSLRAYYSDVLWSVETSEGDGYIYCVIEHQSSAEKNMAFRLMRYATAA
                     MQRHLDKGYDRVPLVVPLLFYHGETSPYPYSLNWLDEFDDPQLARQLYTEAFPLVDIT
                     IVPDDEIMQHRRIALLELIQKHIRDRDLIGMVDRITTLLVRGFTNDSQLQTLFNYLLQ
                     CGDTSRFTRFIEEIAERSPLQKERLMTIAERLRQEGHQIGWQEGKLEGLHEQAIKIAL
                     RMLEQGIDRDQVLAATQLSEADLAANNH"
     gene            complement(8828..10018)
                     /gene="glpC"
                     /locus_tag="J1Y83_002624"
     CDS             complement(8828..10018)
                     /gene="glpC"
                     /locus_tag="J1Y83_002624"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic glycerol-3-phosphate dehydrogenase
                     subunit C"
                     /protein_id="HBA7630764.1"
                     /translation="MNDTSFENCIKCTVCTTACPVSRVNPGYPGPKQAGPDGERLRLK
                     DGALYDEALKYCINCKRCEVACPSDVKISDIIQRARAKYDTTRPSLRNFVLSHTDLMG
                     SVSTPFAPIVNTATSLKPVRQLLDSALKIDHRRTLPKYSFGTFRRWYRSVAAQQAQYK
                     DQVAFFHGCFVNYNHPQLGKDLIKVLNAMGTGVQLLSKEKCCGVPLIANGFTDKARKQ
                     AITNVESIREAVGVKGIPVIATSSTCTFALRDEYPEVLNVDNKGLRDHIELATRWLWR
                     KLDEGKTLPLKPLPLKVVYHTPCHMEKMGWTLYTLELLRKIPGLELTVLDSQCCGIAG
                     TYGFKKENYPTSQAIGAPLFRQIEESGADLVVTDCETCKWQIEMSTSLRCEHPITLLA
                     QALA"
     gene            complement(10015..11274)
                     /gene="glpB"
                     /locus_tag="J1Y83_002625"
     CDS             complement(10015..11274)
                     /gene="glpB"
                     /locus_tag="J1Y83_002625"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase subunit GlpB"
                     /protein_id="HBA7630765.1"
                     /translation="MRFDTVIMGGGLAGLLCGLQLQKHGLRCAIVTRGQSALHFSSGS
                     LDLLSHLPDGQPVTDIHSGLESLRQQAPAHPYTLLGPQRVLDLACQAQALIAESGAQL
                     QGSVELAHQRVTPLGTLRSTWLSAPEVPVWPLPAKKICVVGISGLMDFQAHLAAASLR
                     ELDLAVETAEIELPELDVLRNNATEFRAVNIARFLDNEENWPLLLDALIPVANTCEMI
                     LMPACFGLADDKLWHWLNEKLPCSLMLLPTLPPSVLGIRLQNQLQRQFVRQGGVWMPG
                     DEVKKVTCKNGVVNEIWTRNHADIPLRPRFAVLASGSFFSGGLVAERNGIREPILGLD
                     VLQTATRGEWYKGDFFAPQPWQQFGVTTDEALRPSQAGQTIENLFAIGSVLGGFDPIA
                     QGCGGGVCAVSALHAAQQIAQRAGGQQ"
     gene            complement(11264..12892)
                     /gene="glpA"
                     /locus_tag="J1Y83_002626"
     CDS             complement(11264..12892)
                     /gene="glpA"
                     /locus_tag="J1Y83_002626"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic glycerol-3-phosphate dehydrogenase
                     subunit A"
                     /protein_id="HBA7630766.1"
                     /translation="MKTRDSQSSDVIIIGGGATGAGIARDCALRGLRVILVERHDIAT
                     GATGRNHGLLHSGARYAVTDAESARECISENQILKRIARHCVEPTNGLFITLPEDDLS
                     FQATFIRACEEAGIRAEAIDPQQARIIEPAVNPALIGAVKVPDGTVDPFRLTAANMLD
                     AKEHGAVILTAHEVTGLIREGATVCGVHVRNHLTGETQALHAPVVVNAAGIWGQHIAE
                     YADLRIRMFPAKGSLLIMDHRINQHVINRCRKPSDADILVPGDTISLIGTTSLRIDYN
                     EIDDNRVTAEEVDILLREGEKLAPVMAKTRILRAYSGVRPLVASDDDPSGRNVSRGIV
                     LLDHAERDGLDGFITITGGKLMTYRLMAEWATDAVCRKLGNTRPCTTADTPLPGSQEP
                     AEVTLRKVISLPAPLRGSAVYRHGDRTPAWLSEGRLHRSLVCECEAVTAGEVQYAVEN
                     LNVNSLLDLRRRTRVGMGTCQGELCACRAAGLLQRFNVTTSAQSIEQLSTFLNERWKG
                     VQPIAWGDALRESEFTRWVYQGLCGLEKEQKDAL"
     gene            13165..14523
                     /gene="glpT"
                     /locus_tag="J1Y83_002627"
     CDS             13165..14523
                     /gene="glpT"
                     /locus_tag="J1Y83_002627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012131518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate transporter"
                     /protein_id="HBA7630767.1"
                     /translation="MLSIFKPAPHKARLPAAEIDPTYRRLRWQIFLGIFFGYAAYYLV
                     RKNFALAMPYLVEQGFSRGDLGFALSGISIAYGFSKFIMGSVSDRSNPRVFLPAGLIL
                     AAAVMLFMGFVPWATSSIAVMFVLLFLCGWFQGMGWPPCGRTMVHWWSQKERGGIVSV
                     WNCAHNVGGGIPPLLFLLGMAWFNDWHAALYMPAFCAILVALFAFAMMRDTPQSCGLP
                     PIEEYKNDYPDDYNEKAEQELTAKQIFMQYVLPNKLLWYIAIANVFVYLLRYGILDWS
                     PTYLKEVKHFALDKSSWAYFFYEYAGIPGTLLCGWMSDKVFRGNRGATGVFFMTLVTI
                     ATIVYWMNPAGNPTVDMICMIVIGFLIYGPVMLIGLHALELAPKKAAGTAAGFTGLFG
                     YLGGSVAASAIVGYTVDFFGWDGGFMVMIGGSILAVILLIVVMIGEKRRHEQLLQKRN
                     GG"
     gene            14528..15604
                     /gene="glpQ"
                     /locus_tag="J1Y83_002628"
     CDS             14528..15604
                     /gene="glpQ"
                     /locus_tag="J1Y83_002628"
                     /EC_number="3.1.4.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HBA7630768.1"
                     /translation="MKLTLKNLSMAIMMSTIVMGSSAMAADSNEKIVIAHRGASGYLP
                     EHTLPAKAMAYAQGADYLEQDLVMTKDDHLVVLHDHYLDRVTDVADRFPDRARKDGRY
                     YAIDFTLDEIKSLKFTEGFDIENGKKVQTYPGRFPMGKSDFRVHTFEEEIEFVQGLNH
                     STGKNIGIYPEIKAPWFHHQEGKDIAAKTLEVLKKYGYTGKDDKVYLQCFDADELKRI
                     KNELEPKMGMDLNLVQLIAYTDWNETQQKQPDGSWVNYNYDWMFKPGAMKQVAEYADG
                     IGPDYHMLIEETSQPGNIKLTGMVQDAQQNKLVVHPYTVRSDKLPEYTTDVNQLYDAL
                     YNKAGVNGLFTDFPDKAVKFLNKE"
     gene            complement(15591..15887)
                     /locus_tag="J1Y83_002629"
                     /pseudo
     CDS             complement(15591..15887)
                     /locus_tag="J1Y83_002629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000176735.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            16067..16717
                     /gene="inaA"
                     /locus_tag="J1Y83_002630"
     CDS             16067..16717
                     /gene="inaA"
                     /locus_tag="J1Y83_002630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide kinase InaA"
                     /protein_id="HBA7630769.1"
                     /translation="MAVSAKHDEFNHWWATEGDWVEEPNYRRNGMSGVQCVERNGKKL
                     YVKRMTHHLFHSVRYPFGRPTIVREVAVIKELERAGVIVPKIVFGEAVKIEGEWRALL
                     VTEDMAGFISIADWYARHAVSPYSDEVRQAMLKAVALAFKKMHSVNRQHGCCYVRHIY
                     VKTEGKAEAGFLDLEKSRRRLRRDKAINHDFRQLEKYLEPIPKADWEQVKAYYYAM"
     gene            complement(16771..17025)
                     /gene="yfaE"
                     /locus_tag="J1Y83_002631"
     CDS             complement(16771..17025)
                     /gene="yfaE"
                     /locus_tag="J1Y83_002631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like diferric-tyrosyl radical
                     cofactor maintenance protein YfaE"
                     /protein_id="HBA7630770.1"
                     /translation="MARVTLRITGTQLLCQDEHPSLLAALESHNVAVEYQCREGYCGS
                     CRTRLVAGQVDWIAEPLAFIQPGEILPCCCRAKGDIEIEM"
     gene            complement(17025..18155)
                     /gene="nrdB"
                     /locus_tag="J1Y83_002632"
     CDS             complement(17025..18155)
                     /gene="nrdB"
                     /locus_tag="J1Y83_002632"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006683700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide-diphosphate reductase subunit
                     beta"
                     /protein_id="HBA7630771.1"
                     /translation="MAYTTFSQTKNDQLKEPMFFGQPVNVARYDQQKYDIFEKLIEKQ
                     LSFFWRPEEVDVSRDRIDYQALPEHEKHIFISNLKYQTLLDSIQGRSPNVALLPLISI
                     PELETWVETWAFSETIHSRSYTHIIRNIVNDPSVVFDDIVTNEQIQKRAEGISSYYDE
                     LIEMTSYWHLLGEGTHTVNGKTVTVSLRELKKKLYLCLMSVNALEAIRFYVSFACSFA
                     FAERELMEGNAKIIRLIARDEALHLTGTQHMLNLLRSGADDPEMAEIAEECKQECYDL
                     FVQAAQQEKDWADYLFRDGSMIGLNKDILCQYVEYITNIRMQAVGLDLPFQTRSNPIP
                     WINTWLVSDNVQVAPQEVEVSSYLVGQIDAEVDTDDLSNFQL"
     gene            complement(18345..20630)
                     /gene="nrdA"
                     /locus_tag="J1Y83_002633"
     CDS             complement(18345..20630)
                     /gene="nrdA"
                     /locus_tag="J1Y83_002633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside-diphosphate reductase subunit
                     alpha"
                     /protein_id="HBA7630772.1"
                     /translation="MNQNLLVTKRDGSTERINLDKIHRVLDWAAEGLHNVSISQVELR
                     SHIQFYDGIKTSDIHETIIKAAADLISRDAPDYQYLAARLAIFHLRKKAYGQFEPPAL
                     YDHVVKMVEMGKYDNHLLEDYTEEEFKQMDTFIDHDRDMTFSYAAVKQLEGKYLVQNR
                     VTGEIYESAQFLYILVAACLFSNYPRETRLQYVKRFYDAVSTFKISLPTPIMSGVRTP
                     TRQFSSCVLIECGDSLDSINATSSAIVKYVSQRAGIGINAGRIRALGSPIRGGEAFHT
                     GCIPFYKHFQTAVKSCSQGGVRGGAATLFYPMWHLEVESLLVLKNNRGVEGNRVRHMD
                     YGVQINKLMYTRLLKGEDITLFSPSDVPGLYDAFFADQEEFERLYTKYEKDDSIRKQR
                     VKAVELFSLMMQERASTGRIYIQNVDHCNTHSPFDPAIAPVRQSNLCLEIALPTKPLN
                     DVNDENGEIALCTLSAFNLGAINSLDELEELAILAVRALDALLDYQDYPIPAAKRGAM
                     GRRTLGIGVINFAYYLAKHGKRYSDGSANNLTHKTFEAIQYYLLKASNELAKEQGACP
                     WFNETTYAKGILPIDTYKKDLDAIANEPLHYDWEALRESIKTHGLRNSTLSALMPSET
                     SSQISNATNGIEPPRGYVSIKASKDGILRQVVPDYEHLHDAYELLWEMPGNDGYLQLV
                     GIMQKFIDQSISANTNYDPSRFPSGKVPMQQLLKDLLTAYKFGVKTLYYQNTRDGAED
                     AQDDLVPSIQDDGCESGACKI"
BASE COUNT         4836 a         5128 c         5658 g         5275 t
ORIGIN      
        1 cataggattt ttaatattac accaactgct aattatcgcc agaaaagtcg ctcaatttca
       61 cggtaattgt ctggttgcgc ttgtctatag gtggagttta cgtgttagct ttttgccgat
      121 cttcgttgaa gtcaaaaaaa tatttcatca ttttactggc gctcgctgcg attgccggac
      181 tgggtactca tgccgcctgg agtagcaatg gtttgccacg catcgacaat aaaacactgg
      241 ccagactggc acagcagcac ccggttgtcg ttttgtttcg ccatgctgaa cgttgcgatc
      301 gttcaaccaa tcaatgcttg tcagataaaa caggtattac ggtcaaaggt acccaggatg
      361 cccgtgaact gggcaacgct tttagtgctg atatccctga ttttgatctc tattccagta
      421 ataccgtccg gactattcag tcggcgacct ggttttcggc gggtaaaaag ctgacggtag
      481 ataaacgatt tcttcaatgc ggtaatgaga tttatagtgc aattaaagac ttacaacgca
      541 aagcacctga taaaaatatc gttattttta cccataatca ttgcctgaca tatattgcta
      601 aagataagcg tgatgcgaca tttaaacctg attatctgga tggtttagtc atgcatgtgg
      661 aaaaaggcaa agtttatctg gatggggaat tcgttaacca ctaaattttc aatctgacag
      721 ccagtgatgg ctgtcatcat tgttaaagaa gacctttcaa acgtaacgtt tttcgggtgg
      781 caacattcaa atcataatgc accagatctt caggttttac ccacgcgtag tcctgaaact
      841 cttcgtttat tttcacatct cggttggcag aaacgcagtc aaaaatcagg taaatcatat
      901 aaatctcttc cttgcgacca tctgcatacg tcttggtgcg aatatcatcg ctgaaggtcc
      961 acggcgtgat ttctgtcaaa agcagctgtt ctcccagttc ttcgcgaatt tcgcggcgta
     1021 gtgcctcttc aattcgttcg ccaggctcca cgccgccacc cgaaagcgcc cattgaccgg
     1081 ggaaaacgcc gcgatcgtcg gccattttac acagcaaata agcaccatca ttttgaatca
     1141 aagggcatac aatggtccgt tgtcgcacag gggtattcct tataaataaa aagaaaccat
     1201 gagaatacgg taactcagaa atatgtgaaa gtgacagtaa tctcttttgc gatgataact
     1261 acatttatta ttatttttgt gtgtatcgat aaagtgtgtt tattttttcg gcgtttaatt
     1321 atttaagaag tatttagtta caataagtga ctagatccct tttttcgctc attgaatcct
     1381 ctgcgccagg cagtacaatc ccgcagcttt gggaattttg attaattgct tgtgggaaag
     1441 aggataatga aaaaaattgc gctagcaggt ctggccggaa tgctgttggt ttctgcatcg
     1501 gtcaatgcaa tgagcatcag cggtcaggcg ggtaaagaat acaccaatat tggtgtcggt
     1561 tttgggactg aatcgacggg cctggcgtta agcggtaact ggacacataa cgacgacgac
     1621 ggtgatgccg cgggcgtggg gctggggttg aatctgcctc tcgggccatt aatggcgacc
     1681 gttggcggaa aaggcgttta caccaacccg aacgatggtg atgaaggtta tgcagcggca
     1741 gttggcggtg gtttgcagtg gcaaattggt gacagcttcc gcttgtttgg tgagtattac
     1801 tactctccgg attcgctctc cagcggaatt aaaagttatg aagaagcgaa tgctggcgcg
     1861 cgttacacca ttatgcgtcc agtcagcatt gaggctggct accgttacct gaacctggca
     1921 ggtaaaaatg gcaatcgtga taacgccatt gctgatggtc cgtatgttgg ggtaaatgcc
     1981 agcttctgac gccttccggc gcggcgttct gtcgcgccat tcagtttctt actacgccct
     2041 gtctcccttt gcgttatagt gattcgataa caaaagcagg agataaccat gttaaaagtg
     2101 gaaatgttat ccaccgggga tgaagtgtta cacgggcaaa tcgttgacac taacgctgcc
     2161 tggctggccg attttttctt tcatcagggg ttgccattat ctcgccgcaa tacggtgggg
     2221 gataaccttg atgacttagt caccattctt cgcgaacgta gtcagcacgc cgatgtgctg
     2281 atcgttaacg gcgggctggg accgaccagt gatgatttaa gcgcactcgc cgctgcgaca
     2341 gcaaaaggtg aaggcctggt gctgcatgaa gcctggctca aagagatgga acgctatttc
     2401 cacgaacggg gacgagtaat ggcaccgagc aaccgtaaac aagcggagtt gcctgccagt
     2461 gctgaattta tcaataaccc ggtaggcact gcctgtggtt ttgccataca gcttaatcgt
     2521 tgcctgatgt tctttacccc cggcgtaccg tcagaattta aggtgatggt tgagcacgaa
     2581 atcctgccgc gcctgcgcga gcgtttttct ttaccacagc cgccggtttg cctgcgtttg
     2641 actacctttg gtcgttcgga aagcgatctg gcacaaagcc tggacactct acaactgccg
     2701 ccgggcgtaa caatgggcta tcgctcctca atgccgatta tcgaactgaa actcaccgga
     2761 ccggcaagcg aggaacaggc gatggaaaaa ctgtggctgg acgttaaacg tgttgccgga
     2821 cagagcgtga ttttcgaagg caccgaagga ctgcccgcgc agatcagtcg cgaattgcag
     2881 agccgccagt tcagcctgac gctgagcgag caattcaccg gtggtttatt ggctttgcaa
     2941 ctttctcgcg cagatgctcc attgctggcg tgtgaagtgg ttccttcaca ggaggaaacc
     3001 ctggcgcaaa ctgcgcactg gattacagaa cggcgggcga accattttgc tgggttggca
     3061 ctggctgttt ctggtctgga agatgagcat ctcaactttg cactagccac gccagacggc
     3121 actttcgctt tgcgtgtaca tttcagtgcc acacgctaca gtctggctat tcgccaggaa
     3181 gtctgcgcaa tgatggcgct taatatgcta cgccgttggt taaacggcca ggacatcgcc
     3241 agcgagcatg gctggattga ggttgttgag tccatgacct tatctgtctg attccgccac
     3301 tctgtgccgg tttactgcac cggcacattc cccctccggc aaaatttgtc caacctgttg
     3361 tactcaataa gaaaaaatcc gtgcgctgat tcacaaattt gtgcgtaatt tctccagtcc
     3421 gctggacgag aaacgggatt tgatgcgctt cacaatttta ctccccctga gcaacgagac
     3481 tgattccagt atctaagact ggagtccagt atgttggaat caagcaaagt tcccgccctg
     3541 acacgggcta ttgatattct caatttgatt gcccggattg gcccctgtag tgctgcgacg
     3601 atcattgaca cattggggat ccctaaaagc acagcctatt tgctgcttaa tgaactcagg
     3661 cgtcagcgtt ttcttagcct tgatcaccag gaaaattttt gtttgtggac caggctggtg
     3721 gagctttccg gtcatgcgtt aagcaagatg gatctccggg aactggctcg accgcgtttg
     3781 acgcaactga tggataccac cgggctgtta tgtcacctgg ggattattga taacggcagc
     3841 gcttattaca ttttgaaagt ggagtcatca gccactatca gcgtgcgttc ccatgaagga
     3901 aaaagccttt cgctttatcg ctccggaata ggtaaatgcc tgcttgcctg gcaacctgaa
     3961 gctgtacaga aaagtattat cgaagagctg gtctgggagc agatgacacc caccaccatt
     4021 acccatccac aacggctcta cgaggaactg gcgcgtattc gccgtcaggg ctggagctat
     4081 gacaacggcg aagattatgc cgatgtccgt tgtgtcgctg cgccggtgtt taacgccaat
     4141 aacgagctta ccgctgcgat ttcggtggtc ggtacccgct tacaaatcaa tgaagaatac
     4201 cgtgattatc tggcgggcaa ggccattgcc tgcgccaggg atatttcacg tttgttggga
     4261 tggaaaagtc cctttgactt acaggcctca taacaatgga gaacatcatg accctaccaa
     4321 aaatcaaaca ggttcgcgcc tggtttactg gcggtgcgac agcagaaaaa ggcgctggcg
     4381 gcggtgatta tcacgaccag ggggcgaatc actggattga tgatcatatt gccaccccga
     4441 tgagtaaata ccgcgattac gagcagtcac gccagtcatt tggcattaac gttcttggca
     4501 cgttgattgt tgaagttgaa gcagaaaacg gccagaccgg attcgctgtt tcgacagccg
     4561 gtgaaatggg ctgttttatt gtcgaaaaac atcttaaccg tttcattgag ggtaaatgtg
     4621 tcagtgatat caaactgatc cacgatcaaa tgctcaatgc caccctgtat tactccggct
     4681 ctggtggcct ggtgatgaat acgatttcct gtgtcgatct ggctctgtgg gatctgttcg
     4741 gcaaagtggt cgggcttccg gtttataaac ttttaggcgg cgcagttcgt gatgagattc
     4801 agttctacgc cacaggtgcg cgtccggatc tggcaaaaga gatgggcttt atcggtggca
     4861 aaatgccgac tcactggggg ccacatgatg gcgatgcagg gatccgcaaa gatgccgcta
     4921 tggtcgcgga tatgcgtgaa aaatgcggtg aggatttctg gttaatgctc gactgctgga
     4981 tgagtcagga cgtgaactat gccaccaaac tggcccacgc ttgcgcgccc tataacctga
     5041 aatggatcga agagtgcctg ccgccacagc agtatgaagg ttatcgcgaa ctgaaacgca
     5101 acgcgccagc ggggatgatg gtcaccagtg gtgagcacca ctgcacactg caatcttttc
     5161 gtacgctttc agaaaccggt atcgacatca tgcagccgga tgttggctgg tgcggtggct
     5221 taaccacgct ggtggaaatt gccgcaatcg ccaaatccag ggggcaactg gtggtgccgc
     5281 acggttcgtc tgtttactcc caccatgcgg tgatcacctt caccaatacg ccattcagtg
     5341 aattcctgat gaccagcccg gattgttcaa cgatgcgtcc gcaatttgat ccgattctgc
     5401 ttaatgagcc ggttccggtg aatggtcgta ttcataaatc agtgcttgat aaacccggtt
     5461 tcggcgtcga actcaatcgt gactgcaatt tgaaacgccc ctacagccac taatcactcc
     5521 gggcgttgct gtgacaacgg taacgcccac atattctaag gagcttttta tgagcaccgc
     5581 tttgcttgac gccgtggtga agaaaaaccg cgcgcgttta attccgttta tgttggcgct
     5641 gtatgtgctg gcgtttctcg accgttcgaa tatcggtttt gccaaacaga cctaccagat
     5701 tgataccggg ctgagtaatg aagcttatgc gctgggagca ggcattttct ttgtggtata
     5761 cgcgtttctg ggtgttccgg cgaatctttt gatgcgcaaa ctgggggcaa gaacctggat
     5821 tggtacgaca acactgctgt ggggatttct ttcggcagcc atggcatggg ccgatactga
     5881 agcgaaattt ctgatcgttc gcactctgct tggtgctgcg gaggccgggt tcttccctgg
     5941 tatgatttat ctcacctcgc aatggtttcc gcagcgtaat cgcgccagca ttatggggct
     6001 gttctatatg ggcgcaccgt tggcgttaac actgggatca ccgctttccg gcgcgctgct
     6061 ggagatgcat ggatttatgg ggcatcccgg ctggttctgg atgtttgtga ttgaaggatt
     6121 gctggcagtc ggcgctggtg tattcacatt cttttggctt gatgactcgc cggagcaggc
     6181 tcgttttctg agtaaacaag aaaaaacgtt gcttatcaac caactggcaa gtgaagaaca
     6241 acagaaagtg acttcccgac tgagcgatgc gctgcgtaat gggcgagtct ggcaactggc
     6301 gattatctac ctgaccattc aggtggcggt ttacggatta attttcttcc tgccgacgca
     6361 ggttgctgca ttgctgggga caaaagtggg ctttacggcg tcggtggtca ccgccattcc
     6421 gtgggttgcg gccttgtttg gaacctggct tattccgcgc tattccgata aaaccggcga
     6481 acggcgtaat gtcgcagcgc tgacattact ggcggcgggc attggtattg gtctgtccgg
     6541 gctgctttct ccagtactgg cgatcgtagc gctgtgtgtt gcagccatcg ggtttattgc
     6601 cgtgcagccg gtgttctgga cgatgccgac acaactgctt tccggtacgg cgctggctgc
     6661 ggggattggt tttgtaaacc tgtttggtgc agtgggcggg tttattgccc cgatcctgcg
     6721 cgtgaaagca gaaacgttat tttccagcga tgcggcggga ttactgacgc tggcagcggt
     6781 ggcggtcatc ggttcgctga ttattttcac tctgcgtgta aatcgcactg ttgcgcagac
     6841 cgacgtggca catcattaaa taggttaagg aacacgatga acgcattatt aagcaattcc
     6901 tttaaagaac gtttacgcaa gggcgaagtg caaattggtc tgtggttaag ctcaacgact
     6961 gcctatatgg cagaaattgc cgccacttcc ggttatgact ggttgctgat tgacggggag
     7021 catgcgccaa acaccattca ggatctttat caccagctac aggcggtagc gtcctatacc
     7081 agccaccccg tgatccgtcc ggtggaaggc agtaagccgc tgattaaaca agtcctggat
     7141 attggcgcgc aaactctact gatcccaatg gtcgatactg ccgatcaggc acgtcaggtg
     7201 gtgtctgcca cgcgctatcc tccctacggt gagcgtggtg ttggggccag tgtggcacgg
     7261 gctgctcgct ggggacgcat tgagaattac atggcgcaag tcaacgattc gctttgtctg
     7321 ttggtgcagg tggaaagtaa aacagcactg gataacctgg acgaaatcct cggcgtcgaa
     7381 gggattgatg gcgtgtttat tggacctgcg gatctttctg cctcgttggg ctacccggat
     7441 aacgccgggc acccggaagt gcagcgaatt attgaaacca gcattcggcg gatccgtgct
     7501 gcgggtaaag ctgctggttt tctggctgtg gctcctgata tggcgcagca atgcctggcg
     7561 tggggagcga actttgtcgc tgttggcgtt gacacgatgc tctacagcga tgccctggat
     7621 caacgactgg cgatgtttaa atcaggcaaa aatgggccac gcgtaaaagg tagttattga
     7681 tatcaaaggc ccatggggat cggctgtggg cctgtgttaa ttagtggtta ttcgctgcca
     7741 gatcggcttc gcttagctga gtggccgcga gcacctggtc acgatcaatg ccctgttcca
     7801 gcatgcgcaa agcaatttta atggcttgtt catgcaaacc ttctaattta ccttcctgcc
     7861 agccaatttg atgcccttcc tgccgcagcc gttcagcaat agtcattaat ctctcctttt
     7921 gtagtggtga acgttcggca atctcctcaa taaaacgggt gaaacgggag gtatcgccgc
     7981 attgcagcag ataattaaac agcgtttgta gctggctgtc attagtgaag cctctaacca
     8041 aaagcgtggt gatcctgtcg accataccga ttaaatcgcg gtcgcgaata tgcttttgaa
     8101 tcagttccag cagagctata cgccgatgtt gcatgatctc atcgtcaggt acgatggtga
     8161 tatccaccaa cggaaaagct tcggtgtaca actgccgggc aagttgcgga tcgtcaaact
     8221 catccagcca gttgagtgag tacgggtagg gcgaggtttc gccatgataa aacagcaatg
     8281 gtaccaccag cgggactctg tcatagcctt tgtccaggtg acgctgcatg gcggcagtgg
     8341 catagcgcat tagccgaaaa gccatattct tttctgcaga gctttgatgt tcaatcacgc
     8401 agtagatata accgtcaccc tcgctggttt ccacagacca caaaacatcc gagtagtaag
     8461 cgcgtaaact tttttcaata aagctggtgg gttccaggcg taaggtttgc aggttgcaaa
     8521 gcttgcgcag cggctctggt agatgtattt ccagaaaatc tcgcgccgta tcgggcgaga
     8581 acataaaggt tttaaatacc gcatcatgcg gggaggaggt tgttgattcg gtcatggttg
     8641 tccgtcacct gatatttatg atgacgcgac attacggtta tcccgggcct ttgacatctg
     8701 ccagtttcag cctttgcgag acgttttcag aggaattttc actgcgttgc aacgtataca
     8761 ttgggcacgg aatcgtgtac gcaaaaatgc ccggtaacaa catgcttacc gggcatcaga
     8821 aaggagttta agccagcgcc tgggccagca gcgtaatcgg atgttcgcag cgaaggcttg
     8881 tcgacatctc aatctgccat ttacaggttt cgcagtcggt gaccaccaga tctgcgccgc
     8941 tttcttctat ctggcggaac agtggtgcgc cgatggcttg tgaggtgggg tagttctctt
     9001 ttttgaaacc gtaagtaccc gcaataccgc agcactggga atccagcacc gttaactcaa
     9061 gccccgggat tttacgcaac agctccaggg tgtagagcgt ccagcccatt ttttccatat
     9121 ggcacggagt gtgataaacc acttttagcg gcagcggttt cagcggtaac gttttgcctt
     9181 cgtccagctt gcgccacagc cagcgggtcg ccagttcgat gtgatcgcgt aagcctttgt
     9241 tgtcgacatt cagcacttcc gggtattcgt cgcgcagggc aaatgtacag gttgaggagg
     9301 tggcaatcac cggaatgcct tttactccca ctgcttcgcg gatcgactct acatttgtga
     9361 ttgcctgttt acgtgcttta tcggtaaagc cgttggcgat cagcggtacg ccgcagcatt
     9421 tttctttgct gagcagttgt acaccggtac ccattgcgtt gagcacttta attaaatctt
     9481 tgcctaactg cggatggttg tagttaacga agcagccgtg aaagaaagcc acctggtctt
     9541 tatattgtgc ctgctgggcc gccacgctgc gataccagcg acggaacgta ccgaaggagt
     9601 atttcggtag cgtgcggcga tgatcgattt ttaacgccga atcaagcagc tgccgcactg
     9661 gtttcagcga ggtagcggtg ttgacgattg gagcaaacgg cgtggaaacg ctacccatca
     9721 ggtcggtatg actcaacaca aaattgcgca gcgacgggcg cgtggtgtca tatttcgccc
     9781 gcgcgcgctg gatgatatcg ctaatcttca catcggacgg gcaggcgact tcgcaacgtt
     9841 tgcagttgat gcaatatttc agtgcctcgt catacagtgc gccatctttc aaacgcagac
     9901 gctcgccatc cggcccggct tgttttggcc ccggatatcc cggattcacc cggctcaccg
     9961 ggcaggcggt ggtgcagacg gtgcacttaa tgcagttttc gaagctggtg tcgttcattg
    10021 ttgacctcct gcgcgttggg caatctgttg agcggcatgt aaagcactga cggcacaaac
    10081 gccgccgccg catccctggg cgatgggatc aaatccgccc agcaccgaac cgatggcaaa
    10141 caggttctca atggtttgcc ctgcctgcga cgggcgtagt gcctcatcag tggttacacc
    10201 gaactgctgc cacggttgcg gcgcaaaaaa atcgccctta taccattcac cccgcgtggc
    10261 ggtttgtagc acatcaaggc cgagaatcgg ctctcgaatg ccgttacgtt ccgccaccag
    10321 tccgccgcta aagaaactgc cgctggcgag cacggcaaag cgtggacgca gcgggatatc
    10381 ggcgtgattg cgcgtccaga tttcatttac tacgccattt ttacaggtca cttttttcac
    10441 ttcatcgcct ggcatccaca cgccaccctg gcgcacaaac tggcgctgta actggttttg
    10501 cagacgaatg cccagcacgg aaggcggcag cgttggcaaa agcatcagtg aacaaggtag
    10561 tttttcattc aaccaatgcc acagtttgtc atcggccagg ccgaagcagg cgggcatcag
    10621 gatcatttcg caggtattgg cgacaggaat aagcgcatca agtaacagcg gccagttctc
    10681 ttcgttatca aggaaacggg cgatattcac cgcacgaaac tcggtggcgt tgttgcgcag
    10741 cacatccagt tccggcagtt caatttctgc ggtttcaacg gcaaggtcga gttcgcgcaa
    10801 cgaagccgcc gcaagatgcg cctgaaaatc catcagaccg ctaattccta ctacacatat
    10861 tttcttcgcg ggcagcggcc agacggggac ttctggcgca cttagccagg tagaacgcag
    10921 ggtaccgagc ggggtaacac gctggtgggc cagttctacg ctgccctgta attgcgcacc
    10981 gctttcagcg attaatgcct gcgcctggca agcgagatcg agtacgcgtt gtggcccgag
    11041 aagagtgtaa ggatgggcag gtgcctgctg acgcaaagat tccagtccac tgtgaatgtc
    11101 tgtcaccggt tgaccatccg gcagatggct cagcaaatcc agcgaaccgg atgagaaatg
    11161 cagcgcgctt tgaccacgag tgacaatggc acagcgcagg ccgtgttttt gcagttgcag
    11221 gccacagagt aatccggcga ggccgccgcc cataatgaca gtatcaaagc gcatctttct
    11281 gctccttctc cagaccacac agtccctgat aaacccagcg ggtaaattcg ctttcgcgca
    11341 gtgcgtctcc ccatgctatg ggttgcacgc ctttccagcg ttcgttaagg aaggtggaaa
    11401 gttgctcgat agattgcgcg gacgtcgtga cgttaaaacg ttgcagcagt ccggcagccc
    11461 ggcaggcgca gagttcgccc tggcaggtac ccatccctac acgggtacga cggcgtaaat
    11521 ccagcaggct gttaacgttt aaattttcta ctgcgtactg cacttcaccc gcagttaccg
    11581 cttcgcactc acataccagg ctgcggtgca ggcggccttc gctcagccag gctggtgtgc
    11641 gatcgccgtg ccggtaaact gcggaaccgc gcagcggagc gggcagggag atcactttgc
    11701 gcagcgtgac ttctgccggt tcctgtgagc caggcaacgg cgtgtcggca gtggtacagg
    11761 ggcgcgtgtt gcccagcttg cgacacaccg cgtcagtagc ccattccgcc atcaggcggt
    11821 aggtcatcag tttgccaccg gtgatggtga taaacccgtc cagaccatcg cgttcagcat
    11881 ggtcgagcag cacgatgcca cggctgacgt tacgtccgct cggatcgtca tcgctggcaa
    11941 ccagcgggcg tacgccagag taggctcgca aaatgcgcgt tttcgccatc acgggggcca
    12001 gtttttcccc ttcacgcagc agaatatcaa cttcttctgc cgtcactcga ttatcgtcaa
    12061 tctcgttgta atcaatacgt aaagaggtgg tgccaattag cgaaatggta tcgccaggca
    12121 ccagaatatc ggcgtcagaa ggtttacggc agcggttgat cacatgctga ttaatgcggt
    12181 gatccataat cagcagcgac cctttcgccg ggaacatgcg aatgcgcaga tcggcatatt
    12241 ccgcaatatg ttgcccccag atcccggcgg cattaaccac gacaggcgca tgaagtgcct
    12301 gggtttcgcc ggtgaggtgg ttacgtacat gaacgccgca caccgtcgcg ccttcacgaa
    12361 tcagtcccgt gacttcatga gcagtaagga taaccgcacc gtgttctttg gcatcaagca
    12421 tatttgctgc ggtcagacga aatggatcaa cggtgccatc cggaactttc accgcgccaa
    12481 tcagtgccgg gttaacggca ggttcgataa tgcgcgcttg ctgcgggtct atagcttctg
    12541 ctctgatccc tgcttcttcg caggcgcgga taaaagtggc ctggaaggag aggtcatctt
    12601 ccggcagggt gataaacagg ccgttggtcg gttcaacgca gtgacgcgca atgcgtttca
    12661 ggatctggtt ttcactaata cattcgcggg ccgattccgc atcggttacc gcatagcgcg
    12721 caccgctgtg cagcaggccg tggttacgcc cggtggcacc ggttgcgatg tcgtggcgct
    12781 ctaccaaaat cacgcgcagc ccgcgcaggg cacagtcgcg ggcaatcccg gctcccgttg
    12841 cgccgccgcc aatgataatc acgtcacttg attgcgagtc gcgagttttc attgttatcc
    12901 ctctgaagtt cgttttttac catttagcca tagtaaaaac atgaattgtt tgatttcgcg
    12961 catattcgct cacaattcga aagtgaaacg tgatttcatg cgtcattctg aacatttaaa
    13021 ccatcgtatt taataatgtg tgcggtaatt cacatttaat ttatgaatgt tttcttaaca
    13081 tcgcggcaac tcaagaaacg gcaggttctc ccactgaatc aggctgttaa tcataaataa
    13141 gaccacgggc cacggaggct atcaatgttg agtattttta aaccagcgcc acacaaagcg
    13201 cgcttacctg ctgcggagat cgatccgact tatcgtcgat tgcgctggca aattttcctg
    13261 gggatattct ttggctatgc tgcttactat ttggttcgta agaactttgc gctcgctatg
    13321 ccttatctgg ttgagcaggg attctcacgc ggtgatttag gttttgccct ttcggggatc
    13381 tcgattgctt atggattttc gaaattcatc atgggttcgg tatcggatcg ctcgaatccg
    13441 cgcgttttcc tgcccgcagg tttgattctg gcggcggcag tgatgttgtt tatgggcttt
    13501 gtgccgtggg cgacgtcgag catcgcggtg atgtttgtac tgttgttcct ctgcggttgg
    13561 ttccagggga tggggtggcc gccgtgtggt cgtactatgg tgcactggtg gtcgcagaaa
    13621 gaacgtggcg gcattgtgtc agtgtggaac tgtgcacaca acgtcggtgg tggtattccg
    13681 ccgctgctgt tcctgctggg gatggcctgg ttcaatgact ggcacgcggc gctctatatg
    13741 cccgctttct gcgccattct ggtggcactg ttcgcctttg cgatgatgcg cgataccccg
    13801 caatcctgtg gcctgccgcc gatcgaagag tacaaaaatg attatccgga cgactataac
    13861 gaaaaagcgg aacaggagct gacggcgaag caaatcttca tgcagtacgt gctgccgaac
    13921 aaattgctgt ggtacatcgc tatcgccaac gtgttcgttt atctgctgcg ttacggcatc
    13981 ctcgactggt caccgactta tctgaaagag gtgaaacact tcgcgctgga taaatcctcc
    14041 tgggcctact tcttctatga gtatgcgggc attccgggca ccctgctgtg cggctggatg
    14101 tcggataaag tcttccgtgg caaccgtggg gccaccggcg tcttctttat gacactggta
    14161 accatcgcga ctatcgttta ctggatgaac ccggcaggta acccaaccgt cgatatgatt
    14221 tgtatgattg ttatcggctt cctgatctat ggccctgtga tgctgatcgg tctacatgcg
    14281 ctggaactgg caccgaaaaa agcggcaggt acggcagcgg gttttaccgg gctgtttggt
    14341 tacctcggcg gttcggtggc ggcgagcgcg attgttggct acaccgtgga cttcttcggc
    14401 tgggatggcg gctttatggt aatgattggc ggcagcattc tggcggttat cttgttgatt
    14461 gttgtgatga ttggcgaaaa acgtcgccat gaacaattac tgcaaaaacg caacggaggc
    14521 taatggcatg aaattgacgc tgaaaaacct tagcatggcg atcatgatga gcactatagt
    14581 catgggaagt agtgcaatgg cggcggacag caacgaaaag atagtcatcg cccatcgcgg
    14641 tgccagtgga tatttgccgg agcatacgct gccagcaaaa gcgatggcgt atgcgcaggg
    14701 agcggattat ctggaacagg atttggtgat gaccaaagac gaccatctgg ttgttctgca
    14761 tgaccattat ctcgatcgtg ttactgatgt tgccgatcgt ttcccggatc gggcgcgcaa
    14821 agacggtcgt tactacgcga tagatttcac gctggatgaa attaagtcgc tgaaatttac
    14881 cgaaggtttc gatattgaaa acggtaaaaa agtgcagact tatccggggc gtttcccaat
    14941 gggtaaatct gacttccggg tgcacacctt tgaagaagag attgaatttg ttcaggggtt
    15001 aaaccactct accgggaaaa atatcggtat ctatccagaa attaaagcgc cgtggttcca
    15061 tcatcaggaa gggaaggata ttgcggcaaa aacgctggaa gtgctgaaga aatatggtta
    15121 caccggtaag gacgacaaag tttatttgca atgttttgat gctgatgagc tgaagcgtat
    15181 taagaatgag ctggaaccta aaatgggcat ggatctcaat ctggtacagc tgattgccta
    15241 taccgactgg aatgaaacgc agcagaaaca gccggacgga agctgggtta attacaatta
    15301 cgactggatg tttaagccgg gtgccatgaa acaggtggcg gaatatgctg atggtattgg
    15361 tccggattac catatgttga ttgaggagac atcgcagccg ggtaatatca aactcactgg
    15421 catggtgcaa gatgctcagc agaataaact ggtagtgcat ccttataccg tgcggtcaga
    15481 taaactgcct gaatacacta ctgatgtgaa tcagttatat gatgctctgt ataacaaagc
    15541 gggtgtaaat gggctattta ctgatttccc tgataaggca gtaaaattcc ttaataaaga
    15601 gtaatgctac tgcaggatgg cgttcgtgcg ccatcctgtc tcatatcaaa ttttccactc
    15661 tccactaatt gttctgtgca catttgtagt aaaaacagtg gactgccttc taccggcttt
    15721 tctttagcca gcaacgaaat ctgtcgatag aaggttggtt cgagaggaac ggaacacaat
    15781 tcatctgcaa tggttttcag cgttaactct ggtaataagg cgatgcctaa tccctgtcga
    15841 ataaaattca tcgctgtatc cggctgatta aattaatatt ttatctgctg ggcgcactat
    15901 tagtggttgt ggctggtaga atttatgcgc tgcaaatgaa gggtaaaagc ggtgtcgcgc
    15961 tagtagtggc aaaagaaatt cattaatacg acacgtttca ttaagatttt tcgcaggtaa
    16021 ccacctatag tcattgtcgt taataaaaaa cgacaggcga tagggtatgg cagtttcagc
    16081 aaagcacgac gaatttaatc actggtgggc aacagagggc gactgggttg aagagcctaa
    16141 ctatcgccgt aatggtatga gcggggtgca atgcgtcgag cgcaacggca aaaagctgta
    16201 tgtaaagcgc atgacgcatc atctgtttca ttccgtacgt tatccgttcg gccgaccaac
    16261 gattgtccgt gaggttgcgg taattaaaga actggaacgg gccggtgtca tcgtgccgaa
    16321 gatcgttttt ggtgaagcgg tgaaaattga gggtgaatgg cgcgcgttgc tggtgactga
    16381 agatatggcg gggttcatca gcattgctga ctggtatgcc cggcatgcag tatcgcctta
    16441 ttctgacgaa gtacggcaag ccatgttgaa agcagtggcg ctggcgttta agaaaatgca
    16501 tagcgttaat cgtcagcatg gctgttgtta tgttcgccat atctatgtga aaacagaagg
    16561 taaggcagaa gctggatttc ttgacctgga gaaaagtcgt cgccgcttgc gtcgtgataa
    16621 agcgattaat catgacttcc gccagctgga aaaatatctg gagccgatcc ccaaagcgga
    16681 ctgggagcag gtgaaagcat attactacgc tatgtaatcg ttgttggcca gataagacgt
    16741 ataagcgtcg tatctggcgt taacaccaat tcacatctcg atttcaatat cgccttttgc
    16801 ccggcaacaa cagggcaaaa tttcccccgg ctgaataaaa gctaacggtt cggcaatcca
    16861 gtccacctgg cctgcgacca gtcgggtgcg acaggagccg cagtaacctt cgcggcactg
    16921 gtactcaacc gccacattgt gggattccag cgccgccaga agggaagggt gttcatcctg
    16981 gcacagcagt tgtgtgccag taatgcgcag ggtaacgcgg gccatcagag ctggaagtta
    17041 ctcaaatcgt cggtgtccac ttccgcgtca atctgcccga ccagataaga actgacttcc
    17101 acttcctgcg gagcaacctg cacgttatca gacaccagcc aggtgttgat ccacgggatc
    17161 gggttggagc gcgtctggaa cggcagatcc aaaccgactg cctgcatacg gatattggtg
    17221 atgtattcaa cgtactggca gagaatgtct ttattcagac caatcatcga accatcacgg
    17281 aacagataat ccgcccagtc tttctcttgc tgtgccgcct ggacaaacag gtcatagcac
    17341 tcctgcttac attcttcggc aatttccgcc atctccggat catccgcgcc gctgcgcagc
    17401 agattcagca tatgctgggt gccggtcagg tgcagggctt cgtcgcgggc aatcaggcga
    17461 ataattttgg cgttaccttc catcagctca cgttcagcga atgcgaaaga gcaggcaaag
    17521 ctgacgtaga aacggatcgc ttccagcgcg ttaacgctca tcaggcagag atacagtttt
    17581 ttcttcagct cgcgcaggct aacggttacg gttttaccgt taacggtgtg ggtgccttcg
    17641 cccagcagat gccagtagct ggtcatttcg atcagttcat cgtaatagct ggagatccct
    17701 tccgcacgtt tctggatctg ctcgttggtg acgatatcgt caaagacaac tgacggatcg
    17761 ttaacgatat tacggatgat atgggtatag gagcgggaat gaatcgtttc tgagaacgcc
    17821 caggtttcta cccaggtttc cagttccggg atcgagatca gcggcagcag tgccacgttc
    17881 gggctgcgac cctgaatgga atccagcagc gtctgatatt tcaggttgct gataaagatg
    17941 tgtttttcgt gttcaggcag tgcctggtaa tcgatacggt cgcgggagac gtcaacttct
    18001 tccggacgcc agaagaaaga gagctgcttt tcgatcagct tttcgaagat gtcatatttt
    18061 tgctgatcgt agcgagccac gttgaccggc tgaccaaaga acattggttc tttgagctga
    18121 tcatttttcg tctgtgaaaa ggtggtatat gccatgaatg tgtcctgtta ggagttttat
    18181 gccggatgcg cttcgcttat ccggcctaca aaccgcatcg taggctgggt agggcgttca
    18241 tgccgcatct gggcatccag tgccggatgc gcttcgctta tccggcctac aaattggacc
    18301 cgtaggccgg gtagggcgct cacgccgcat ccggcatcac aatatcagat cttacatgcg
    18361 ccgctttcgc agccatcgtc ctggattgac ggcaccagat cgtcctgcgc gtcttctgcg
    18421 ccgtcacggg tgttctgata atacagcgtt ttgaccccga atttgtaggc agtgagcagg
    18481 tctttcagca actgctgcat cggcactttt cctgacggga aacgtgacgg atcgtagttg
    18541 gtgttagcag agatcgactg atcgataaat ttctgcatga tacccaccag ttgcaggtag
    18601 ccatcgttac ccggcatttc ccacagcagc tcataggcgt cgtgcagatg ctcgtagtcc
    18661 ggcaccacct ggcgcagaat accgtctttc gacgctttga tgctgacgta accgcgcggc
    18721 ggttcaatac cgttagtggc gttagagatc tgcgaagaag tctcggacgg catcagggca
    18781 gaaagcgtgg agttacgcag gccgtgcgtt ttgattgact cacgcagtgc ttcccagtcg
    18841 taatgcagcg gctcattagc gatggcatcc agatctttct tataggtatc gatcggcagg
    18901 atccctttcg cgtaagtggt ttcgttaaac cacgggcacg cgccttgctc tttcgccagc
    18961 tcattagacg ctttcagcag gtaatactga atggcttcga aggttttatg cgtcaggttg
    19021 ttggcgctgc cgtcggagta gcgtttaccg tgcttcgcca ggtagtaagc gaagttgatc
    19081 acaccaatac ccagcgtacg acgacccatc gctccacgtt tggcggccgg gatcgggtaa
    19141 tcctgataat ccagcagcgc gtcgagtgcg cgtactgcca gaattgccag ctcttccagt
    19201 tcatccaggc tgttaattgc gcccaggttg aaagcagaca gcgtacacag cgcgatttca
    19261 ccgttctcgt cgttgacgtc gttcagcggt ttggtcggca gggcgatttc caggcacagg
    19321 ttagactggc gcactggcgc gatggccgga tcaaacgggc tatgggtatt gcagtggtca
    19381 acgttctgaa tatagatacg accggtagac gcacgttcct gcatcatcag cgagaacagc
    19441 tcaacggctt tcacacgttg cttgcggatg ctgtcgtctt tctcatattt ggtatacagg
    19501 cgttcaaact cttcctgatc ggcgaagaac gcgtcataca gccccggtac gtcggacggg
    19561 ctgaacagag tgatatcttc acctttcagc agacgggtat acatcagttt gttgatttga
    19621 accccgtagt ccatatgacg cacgcggttg ccttccacac cacggttgtt tttcaacacc
    19681 agcaggcttt ccacttccag atgccacatc gggtagaaca gcgttgccgc accgccgcgc
    19741 acaccgccct gagagcagga tttcaccgct gtctggaaat gtttgtagaa cggaatacaa
    19801 ccggtatgga acgcttcgcc accgcgaatc gggctaccca gcgcacgaat acgcccggcg
    19861 ttgatgccga tcccggcacg ctgggaaacg tatttaacaa tcgcgctgga ggtggcgtta
    19921 atggaatcca ggctgtcgcc gcactcgatc agcacgcatg agctgaactg acgagtcggg
    19981 gtacgcacgc cggacatgat tggcgtcggc agcgaaattt taaatgtgga aaccgcgtcg
    20041 taaaaacgct tcacgtattg caggcgcgtt tcacgcgggt agttcgagaa caggcacgcg
    20101 gcaaccagaa tataaaggaa ctgggcgctt tcgtagattt cgccggttac gcggttctgt
    20161 accagatatt tgccttccag ctgcttaacg gcagcataag agaaggtcat atcacggtcg
    20221 tgatcgataa acgtgtccat ctgcttgaac tcttcttccg tgtagtcttc cagcagatga
    20281 ttatcgtatt tgcccatctc gaccattttc accacatggt cgtacagcgc aggcggctca
    20341 aactgaccgt aggctttttt acgcaggtgg aagatcgcca gacgtgcggc gagatactga
    20401 taatccggcg catcacggga gatcaggtct gcggcagcct tgataatagt ttcgtggatg
    20461 tcagaggtct tgataccgtc ataaaactga atgtgggagc gcagctcgac ctgggaaatc
    20521 gaaacgttat gcagtccttc tgccgcccaa tccagaacgc gatggatttt gtcgagattg
    20581 atgcgctctg tgctaccgtc gcgctttgtc accagcagat tctgattcat gtatgtcgta
    20641 cctgtttttc aatagcttca tgcagactac ctgtagtgag ggaagctgct aagaacacta
    20701 tatatagggg gtatgtttgg gattcactgc aagatagtgt gaaactggcc ctcttgcaag
    20761 tgcataactt tgtggataac tcaggaagga aaaagtggct tccgcgcacc ttaggtcaga
    20821 caaggtgtcc gggaaagtca atgggaagag aaaaatttgt taaaaataac cgttcggtga
    20881 attcttgaac ggtaaaa
//
LOCUS       DADSYM010000083        20875 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2415
            SAMN08039579-rid11723173.denovo.083, whole genome shotgun sequence.
ACCESSION   DADSYM010000083 DADSYM010000000
VERSION     DADSYM010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039579
            Sequence Read Archive: SRR6362533
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 20875)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20875)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980162.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 03:39:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,484
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 4,347
            CDSs (with protein)               :: 4,347
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 43 of 85
            Pseudo Genes (incomplete)         :: 37 of 85
            Pseudo Genes (internal stop)      :: 19 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20875
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039579-rid11723173.denovo.083"
                     /strain="LGE2415"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8302 N 95.9628 W"
                     /collection_date="2015-08-24"
     gene            complement(61..2508)
                     /gene="glgP"
                     /locus_tag="J1Y83_002634"
     CDS             complement(61..2508)
                     /gene="glgP"
                     /locus_tag="J1Y83_002634"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen phosphorylase"
                     /protein_id="HBA7630773.1"
                     /translation="MNAPFTYSSPTLSVEALKHSIAYKLMFTIGKDPVVANKHEWLNA
                     TLFAVRDRLVERWLRSNRAQLSQETRQVYYLSMEFLIGRTLSNAMLSLGIYEDVQGAL
                     EAMGLNLEELIDEENDPGLGNGGLGRLAACFLDSLATLGLPGRGYGIRYDYGMFKQNI
                     VNGSQKESPDYWLEYGNPWEFKRHNTRYKVRFGGRIQQEGKKTRWIETEEILGVAYDQ
                     IIPGYDTDATNTLRLWSAQASSEINLGKFNQGDYFAAVEDKNHSENVSRVLYPDDSTY
                     SGRELRLRQEYFLVSSTIQDILSRHYQLHKTYDNLADKIAIHLNDTHPVLSIPEMMRL
                     LIDEHQFSWDDAFEVCCQVFSYTNHTLMSEALETWPVDMLGKILPRHLQIIFEINDYF
                     LKTLQEQYPNDTDLLGRASIIDESNGRRVRMAWLAVVVSHKVNGVSELHSNLMVQSLF
                     ADFAKIFPGRFTNVTNGVTPRRWLAVANPSLSAVLDEHLGRNWRTDLSLLNDLQQHCD
                     FPMVNHAVHQAKLENKKRLAEYIAQQLNVVVNPKALFDVQIKRIHEYKRQLMNVLHVI
                     TRYNRIKADPDAKWVPRVNIFGGKAASAYYMAKHIIHLINDVAKVINNDPQIGDKLKV
                     VFIPNYSVSLAQLIIPAADLSEQISLAGTEASGTSNMKFALNGALTIGTLDGANVEML
                     DHVGADNIFIFGNTAEEVEELRRQGYKPREYYEKDEELHQVLTQIGSGVFSPEDPGRY
                     RDLVDSLINFGDHYQVLADYRSYVDCQDKVDELYERQEEWTAKAMLNIANMGYFSSDR
                     TIKEYADHIWHIDPVRL"
     gene            complement(2527..3960)
                     /gene="glgA"
                     /locus_tag="J1Y83_002635"
     CDS             complement(2527..3960)
                     /gene="glgA"
                     /locus_tag="J1Y83_002635"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase GlgA"
                     /protein_id="HBA7630774.1"
                     /translation="MQVLHVCSEMFPLLKTGGLADVIGALPAAQIADGVDARVLLPAF
                     PDIRRGVTDAQVVSRRDTFAGHITLLFGHYNGVGIYLIDAPHLYDRPGSPYHDTNLFA
                     YTDNVLRFALLGWVGAEMASGLDPFWRPDVVHAHDWHAGLAPAYLAARGRPAKSVFTV
                     HNLAYQGMFYAHHMNDIQLPWSFFNIHGLEFNGQISFLKAGLYYADHITAVSPTYARE
                     ITEPQFAYGMEGLLQQRHREGRLSGVLNGVDEKIWSPETDLLLASRYTRDTLEDKAEN
                     KRQLQIAMGLKVDDKVPLFAVVSRLTSQKGLDLVLEALPGLLEQGGQLALLGAGDPVL
                     QEGFLAAAAEYPGQVGVQIGYHEAFSHRIMGGADVILVPSRFEPCGLTQLYGLKYGTL
                     PLVRRTGGLADTVSDCSLENLADGVASGFVFEDSNAWSLLRAIRRAFVLWSRPSLWRF
                     VQRQAMAMDFSWQVAAKSYRELYYRLK"
     gene            complement(3960..5255)
                     /gene="glgC"
                     /locus_tag="J1Y83_002636"
     CDS             complement(3960..5255)
                     /gene="glgC"
                     /locus_tag="J1Y83_002636"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HBA7630775.1"
                     /translation="MVSLEKNDHLMLARQLPLKSVALILAGGRGTRLKDLTNKRAKPA
                     VHFGGKFRIIDFALSNCINSGIRRMGVITQYQSHTLVQHIQRGWSFFNEEMNEFVDLL
                     PAQQRMKGENWYRGTADAVTQNLDIIRRYKAEYVVILAGDHIYKQDYSRMLIDHVEKG
                     ARCTVACMPVPIEEASAFGVMAVDENDKIIEFVEKPANPPSMPNDPSKSLASMGIYVF
                     DADYLYELLEEDDRDENSSHDFGKDLIPKITEAGLAYAHPFPLSCVQSDPDAEPYWRD
                     VGTLEAYWKANLDLASVVPELDMYDRNWPIRTYNESLPPAKFVQDRSGSHGMTLNSLV
                     SGGCVISGSVVVQSVLFSRVRVNSFCNIDSAVLLPEVWVGRSCRLRRCVIDRACVIPE
                     GMVIGENAEEDARRFYRSEEGIVLVTREMLRKLGHKQER"
     gene            complement(5273..7246)
                     /gene="glgX"
                     /locus_tag="J1Y83_002637"
     CDS             complement(5273..7246)
                     /gene="glgX"
                     /locus_tag="J1Y83_002637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching protein GlgX"
                     /protein_id="HBA7630776.1"
                     /translation="MTQLAIGKPTPLGAHYDGQGVNFTLFSAHAERVELCVFDANGQE
                     HRYDLPGHSGDIWHGYLPDARPGLRYGYRVHGPWQPAEGHRFNPAKLLIDPCARQIDG
                     EFKDNPLLHAGHNEPDYRDNAAIAPKCVVVVDHYDWEDDAPPRTPWGSTIIYEAHVKG
                     LTYLHPEIPVEIRGTYKALGHPVMINYLKQLGITALELLPVAQFASEPRLQRMGLSNY
                     WGYNPVAMFALHPAYACSPETALDEFRDAIKALHKAGIEVILDIVLNHSAELDLDGPL
                     FSLRGIDNRSYYWIREDGDYHNWTGCGNTLNLSHPAVVDYASACLRYWVETCHVDGFR
                     FDLAAIMGRTPEFRQDAPLFTAIQNCPVLSQVKLIAEPWDIAPGGYQVGNFPPLFAEW
                     NDHFRDAARRFWLHYDLPLGAFAGRFAASSDVFKRNDRLPSAAINLVTAHDGFTLRDC
                     VCFNHKHNEANGEENRDGTNNNYSNNHGKEGLGGTLDLVERRRDSIHALLTTLLLSQG
                     TPMLLAGDEHGHSQHGNNNAYCQDNQLTWLDWSQASSGLTAFTAALIHLRKRIPALME
                     NRWWEEGDGNVRWLNRYAQPLSTDEWQNGPKQLQILLSDRFLIAINATLEVTEIVLPA
                     GEWHAIPPFAGEDNPVITAVWQGPAHGLCVFQR"
     gene            complement(7243..9429)
                     /gene="glgB"
                     /locus_tag="J1Y83_002638"
     CDS             complement(7243..9429)
                     /gene="glgB"
                     /locus_tag="J1Y83_002638"
                     /EC_number="2.4.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching enzyme"
                     /protein_id="HBA7630777.1"
                     /translation="MSDRIDRDVINALIAGHFADPFSVLGMHKTTAGLEVRALLPDAT
                     DVWVIEPKTGRKLAKLECLDSRGFFSGVIPRRKNFFRYQLAVVWHGQQNLIDDPYRFG
                     PLIQEMDAWLLSEGTHLRPYETLGAHADTMDGVTGTRFSVWAPNARRVSVVGQFNYWD
                     GRRHPMRLRKESGIWELFIPGAHNGQLYKYEMIDANGNLRLKSDPYAFEAQMRPETAS
                     LICGLPEKVVQTEERKKANQFDAPISIYEVHLGSWRRHTDNNFWLSYRELADQLVPYA
                     KWMGFTHLELLPINEHPFDGSWGYQPTGLYAPTRRFGTRDDFRYFIDAAHAAGLNVIL
                     DWVPGHFPTDDFALAEFDGTNLYEHSDPREGYHQDWNTLIYNYGRREVSNFLVGNALY
                     WIERFGIDALRVDAVASMIYRDYSRKEGEWIPNEFGGRENLEAIEFLRNTNRILGEQV
                     SGAVTMAEESTDFPGVSRPQDMGGLGFWYKWNLGWMHDTLDYMKLDPIYRQYHHDKLT
                     FGMLYNYTENFVLPLSHDEVVHGKKSMLDRMPGDAWQKFANLRAYYGWMWAFPGKKLL
                     FMGNEFAQGREWNHDASLDWHLLEGGDNWHHGVQRLVRDLNHTYRHHKAMHELDFDPY
                     GFEWLVVDDKERSVLIFVRRDKEGNEIIVASNFTPVPRHDYRFGINQPGKWREILNTD
                     SMHYHGSNAGNGGAVHSDEIASHGRQHSLGLTLPPLATIWLVREAE"
     gene            complement(9702..10805)
                     /gene="asd"
                     /locus_tag="J1Y83_002639"
     CDS             complement(9702..10805)
                     /gene="asd"
                     /locus_tag="J1Y83_002639"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HBA7630778.1"
                     /translation="MKNVGFIGWRGMVGSVLMQRMVEERDFDAIRPVFFSTSQLGQAA
                     PSFGGTTGTLQDAFDLEALKALDIIVTCQGGDYTNEIYPKLRESGWQGYWIDAASSLR
                     MKDDAIIILDPVNQDVITDGLNNGIRTFVGGNCTVSLMLMSLGGLFANDLVDWVSVAT
                     YQAASGGGARHMRELLTQMGHLYGHVADELANPSSAILDIERKVTTLTRSGELPVDNF
                     GVPLAGSLIPWIDKQLDNGQSREEWKGQAETNKILNTSSVIPVDGLCVRVGALRCHSQ
                     AFTIKLKKDVSIPTVEELLAAHNPWAKVVPNDREITMRELTPGAVTGTLTTPVGRLRK
                     LNMGPEFLSAFTVGDQLLWGAAEPLRRMLRQLA"
     gene            10997..11590
                     /gene="yhgN"
                     /locus_tag="J1Y83_002640"
     CDS             10997..11590
                     /gene="yhgN"
                     /locus_tag="J1Y83_002640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter YhgN"
                     /protein_id="HBA7630779.1"
                     /translation="MNEIISATVLLILIMDPLGNLPIFMSVLKHTEPKRRRAIMVREL
                     LIALLVMLVFLFAGEKILAFLSLRAETVSISGGIILFLIAIKMIFPSASGNSSGLPAG
                     EEPFIVPLAIPLVAGPTILATLMLLSHQYPNQMGHLVIALLLAWGGTFVILLQSSLFL
                     RLLGEKGVNALERLMGLILVMMATQMFLDGIRMWMKG"
     gene            complement(11636..12931)
                     /locus_tag="J1Y83_002641"
     CDS             complement(11636..12931)
                     /locus_tag="J1Y83_002641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312307.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HBA7630780.1"
                     /translation="MDADSSDSTVTPGRLPDGLSSCPCFYLGKKLMNLSANAQATLLL
                     TSDFSRAAASEYKPLSNSEWGKFALWLKHQRISPAELLMPQPQEKLTGWSDPRISQER
                     ILGLLARGHSLALAVDKWQRAGLWILTRGDADYPVRLKNRLRTDAPPVLFGCGNKALL
                     QAEGMAIVGSRDAPTDDLRYTQQLAAKLAQQGICVISGGARGIDECAMASVLEVGGTA
                     VGVLADSLLKTSTLVKWREGLIAGNLVLISPFYPEVRFTVGNAMARNKYIYCLAESAM
                     VVRAGMTGGTITGAMEALKHQWLPMQVKPNQDMQSANSRLVENGASWSAEQAENVTIR
                     LPDVPGLMYDRALRNAQPELFSLHEDDANYAVMPAHTPVDFYQLFVAELAILAKESIS
                     IERLASCTGLTIEQISVWLNRAEEEGRVIRLGEGHYQFR"
     gene            complement(12838..14934)
                     /locus_tag="J1Y83_002642"
     CDS             complement(12838..14934)
                     /locus_tag="J1Y83_002642"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010349194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RecQ family ATP-dependent DNA helicase"
                     /protein_id="HBA7630781.1"
                     /translation="MEKHGAELLLQRMLSNTSATFREGQWEAIDAVVNQRRKLLVVQR
                     TGWGKSAVYFIASKIFRDRGAGPTIIISPLLALMRNQVAAAERLGITAETLNSTNREE
                     WQRISDKLLQGGVDCLLISPERLANQDFLETVLYPVADRIGLLVVDEAHCISDWGHDF
                     RPDYRRILDILRQLPANTPILGTTATANNRVVEDIRQQLGDIVIQRGTLARESLALDA
                     LVLGEQSSRLAWLATVIPQFSKSGIVYTLTTRDAELVADWLRKNGISAFAYYSGVTCE
                     GAEDSNTAREYLEQALLANKIKVLVATTALGMGFDKPDLGFVIHYQMPGSIVGYYQQV
                     GRAGRAIDSAVGILLCGGEDRAIHQFFRESAFPAEAQIHEILNVLSENDGLTLRGIEQ
                     RTNLRYGQIEKALKLLVAENPSPVVYTEKLWRRTIVSFSPDHERINHLMNQRKSELAD
                     VESYITTKECKMQFLRRALDEPSAERCGKCSSCLQHPLLSPDIDSGLLHAANLFIKHA
                     DLPLNLNKQVASGAFTQYGFKGNLPAGLQGSTGRILSRWGDSGWGKQVAQEKKTGRFS
                     DELVEACAEMVRQRWNPHPEPTWVCCVPSLRHLDLVPDFARRLAAKLGLPFIDAIEKV
                     VDNPPQKMQQNRFHQCQNLDGAFVITPPLMPGPALLVDDIVDSAWTLTVLTALLRQAG
                     CPTVYPLALASTSVKN"
     gene            complement(15075..16415)
                     /gene="gntU"
                     /locus_tag="J1Y83_002643"
     CDS             complement(15075..16415)
                     /gene="gntU"
                     /locus_tag="J1Y83_002643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate transporter"
                     /protein_id="HBA7630782.1"
                     /translation="MTTLTLVLTAVGSVLLLLFLVMKARMHAFLALMVVSMGAGLFSG
                     MPLDKIAATMEKGMGGTLGFLAVVVALGAMFGKILHETGAVDQIAVKMLKSFGHSRVH
                     YAIGLAGLVCALPLFFEVAIVLLISVAFSMARHTGTNLVKLVIPLFAGVAAAAAFLVP
                     GPAPMLLASQMNADFGWMILIGLCAAIPGMIIAGPLWGNFISRYVELHIPDDISEPHL
                     GEGKMPSFGFSLSLILLPLVLVGLKTIAARFVPEGSTAYEWFEFIGHPFTAILVACLV
                     AIYGLAMRQGMPKDKVMEICGHALQPAGIILLVIGAGGVFKQVLVDSGVGPALGEALT
                     GMGLPIAITCFVLAAAVRIIQGSATVACLTAVGLVMPVIEQLNYSGAQMAALSICIAG
                     GSIVVSHVNDAGFWLFGKFTGATEAETLKTWTMMETILGTVGAIVGMIAFQLLS"
     gene            complement(16419..16946)
                     /gene="gntK"
                     /locus_tag="J1Y83_002644"
     CDS             complement(16419..16946)
                     /gene="gntK"
                     /locus_tag="J1Y83_002644"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HBA7630783.1"
                     /translation="MSTTNHDHHIYVLMGVSGSGKSAVASEVAHQLHAAFLDGDFLHP
                     RCNIEKMASGEPLNDDDRKPWLQALNDAAFAMQRTNKVSLIVCSALKKHYRDLLREGN
                     PNLSFIYLKGDFDVIESRLKARKGHFFKTQMLVTQFETLQEPGADETDVLVVDIDQPL
                     EGVVASTIEVIKKGK"
     gene            complement(17085..18080)
                     /locus_tag="J1Y83_002645"
     CDS             complement(17085..18080)
                     /locus_tag="J1Y83_002645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001693277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator GntR"
                     /protein_id="HBA7630784.1"
                     /translation="MKKKRPVLQDVADRVGVTKMTVSRFLRNPEQVSVALRGKIAAAL
                     DELGYIPNRAPDILSNATSRAIGVLLPSLTNQVFAEVLRGIESVTDAHGYQTMLAHYG
                     YKPEMEQERLESMLSWNIDGLILTERTHTPRTLKMIEVAGIPVVELMDSKSPCLDIAV
                     GFDNFEAARQMTTAIIARGHRHIAYLGARLDERTIIKQKGYEQAMLDAGLVPYSVMVE
                     QSSSYSSGIELIRQARREYPQLDGVFCTNDDLAVGAAFECQRLGLKVPDDMAIAGFHG
                     HDIGQVMEPRLASVLTPRERMGSIGAERLLARIRGESVTPKMLDLGFTLSPGGSI"
     gene            complement(18173..18868)
                     /locus_tag="J1Y83_002646"
     CDS             complement(18173..18868)
                     /locus_tag="J1Y83_002646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HBA7630785.1"
                     /translation="MIYLRKANERGHANHGWLDSWHTFSFANYYDPNFMGFSALRVIN
                     DDVIEAGQGFGTHPHKDMEILTYVLEGTVEHQDSMGNKEQVPAGEFQIMSAGTGIRHS
                     EYNPSSTERLHLYQIWIMPEENGITPRYEQRRFDAVQGKQLVLSPDARDGSLKVHQDM
                     ELYRWALVKDEQSVHQIAAERRVWIQVVKGNVTINGVKASTSDGLAIWDEQAISIHAD
                     SDSEILLFDLPPV"
     gene            complement(18991..20028)
                     /locus_tag="J1Y83_002647"
     CDS             complement(18991..20028)
                     /locus_tag="J1Y83_002647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HBA7630786.1"
                     /translation="MVINCAFIGFGKSTTRYHLPYVLNRKDSWHVAHIFRRHAKPEEQ
                     APIYSHIHFTSDLDEVLNDPDVKLVVVCTHADSHFEYAKRALEAGKNVLVEKPFTPTI
                     AQAKELFALAKSKGLIVTPYQNRRFDSCFLTAKKAIESGKLGEIVEVESHFDYYRPVA
                     ETKPGLPQDGAFYGLGVHTMDQIISLFGRPDHVAYDIRSLRNKANPDDTFEAQLFYGD
                     LKAIVKTSHLVKIDYPKFIVHGKKGSFIKYGIDQQETSLKANIMPGEPGFAADDSVGV
                     LEYVNDEGVTVREEMKPEMGDYGRVYDALYQTITNGAPNYVKESEVLTNLEILERGFE
                     QASPSAVTLAR"
     gene            20362..20850
                     /gene="yhhY"
                     /locus_tag="J1Y83_002648"
     CDS             20362..20850
                     /gene="yhhY"
                     /locus_tag="J1Y83_002648"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HBA7630787.1"
                     /translation="MSEIVIRHAETRDHEAIRQIHAQPEVYYNTLQVPHPSEQMWLER
                     LTARPGIKQLVACIDGIVVGHLTIDVQQRPRRSHVADFGICVDSRWKNRGVASALMRE
                     MIEMCDNWLRVDRIELTVFVDNAPAIKVYKKFGFEIEGTGKKYALRNGEYVDAYYMAR
                     VK"
BASE COUNT         5143 a         5703 c         5126 g         4903 t
ORIGIN      
        1 accgtgagtt ccacgctgta ttacttcagc ggaacacacg gtatttagcc gtatttgaac
       61 ttacaatctc accggatcaa tatgccagat atgatcggcg tactctttga tcgtacggtc
      121 agaagagaag tagcccatat tggcaatgtt cagcatcgct tttgcggtcc actcttcctg
      181 acgctcgtag agttcgtcga ctttatcctg acaatcgaca tagctgcgat aatccgccag
      241 tacctggtag tgatcgccga agttgatcag cgaatcgacc agatcgcgat agcgacccgg
      301 atcttccgga ctgaatacac cgctgccgat ttgcgtcagc acctgatgca gctcctcatc
      361 tttctcgtag tattcacgcg gtttgtagcc ctgacgacgc agttcttcca cttcttccgc
      421 tgtgttacca aagataaaga tattgtcagc accgacatga tccagcatct cgacattcgc
      481 accgtccagc gtaccgatag tcagcgcgcc gttaagcgca aacttcatgt tactggtgcc
      541 ggaagcttcc gtccccgcca gcgaaatctg ttcagacaga tcggccgccg gaatgatcaa
      601 ctgcgccagg ctaacactgt agttcgggat gaacacaact ttcagcttgt cgccaatctg
      661 cggatcgttg ttgatcactt tcgctacgtc attgatcaaa tgaataatgt gcttcgccat
      721 gtaataggcc gaagccgcct taccaccaaa aatattcacg cgcggtaccc acttcgcatc
      781 cgggtcggcc ttgatgcggt tatagcgggt gatcacgtgc aacacattca tcaattgacg
      841 tttgtattcg tgaatacgtt tgatttgcac atcgaacagc gctttcggat ttaccaccac
      901 attcagctgc tgggcgatat actccgccag acgctttttg ttctccagct tcgcctgatg
      961 caccgcgtga ttaaccattg ggaaatcaca gtgttgttgc agatcattaa gcaggctaag
     1021 gtcggtgcgc cagttgcggc ccaggtgttc gtccaacacg gctgaaagcg atgggttcgc
     1081 taccgccaac cagcgacgcg gcgtcacacc gttggtgacg ttggtgaaac gacccgggaa
     1141 gattttcgca aagtcggcaa atagcgattg caccatcagg ttagagtgca gctccgatac
     1201 accgttaact ttgtggctca ccacaaccgc cagccaggcc atacgcacac gacgaccgtt
     1261 ggattcatca atgatcgacg cccgtcccag cagatcggta tcgttcggat actgttcctg
     1321 caaggttttc aggaaatagt cgttgatttc aaagatgatc tgcaggtgac gcggcagaat
     1381 tttacccagc atatcgactg gccaggtttc cagcgcctcg ctcattagcg tgtggttggt
     1441 gtaggagaag acctgacaac acacttcaaa cgcgtcgtcc cagctaaatt ggtgctcatc
     1501 gatcagcaga cgcatcatct caggaatcga cagcaccgga tgggtatcgt tgaggtgaat
     1561 cgcaatttta tccgccaggt tatcgtaggt tttatgcaac tgataatggc ggcttaaaat
     1621 gtcctgaatg gtcgaggaaa ccaggaagta ttcctgacgc agtcgcagct cacgcccgga
     1681 gtaggtggag tcgtccggat acagtacgcg agatacgttc tcggagtggt ttttatcttc
     1741 cactgccgcg aagtagtcgc cctggttgaa tttaccgagg ttaatttcgc tactggcttg
     1801 ggcactccac aaacgcaacg tgttggtcgc gtcggtgtcg taacctggga ttatctgatc
     1861 gtaagctact cccagaatct cttcggtttc aatccagcgc gtttttttac cttcctgctg
     1921 aatgcgaccg ccaaaacgga ctttatagcg cgtgttgtgg cgtttgaatt cccacgggtt
     1981 accgtattcc agccagtagt caggcgactc tttctggcta ccgttaacga tgttctgctt
     2041 gaacataccg tagtcatagc ggatgccgta accgcgcccc ggcaacccta acgtcgccag
     2101 agaatcgagg aagcaagccg ccagacggcc caggccaccg ttaccgaggc ccgggtcatt
     2161 ttcttcatca atcagctctt cgagatttaa tcccatcgct tccagtgcgc cctgtacatc
     2221 ttcgtaaatt cctagcgaca acatggcgtt ggaaagcgta cggccaatca aaaactccat
     2281 cgacaggtag taaacctgac gcgtttcttg cgacaactgg gcacggtttg agcgtaacca
     2341 gcgctccacg agacgatcgc gcacagcaaa taatgttgcg ttcagccatt catgtttatt
     2401 ggcgacgacc ggatcctttc caatcgtaaa catcagcttg taagcgatag agtgcttaag
     2461 ggcttctacg ctaagcgtgg gcgatgaata tgtaaacgga gcattcatat aggcgtttcc
     2521 tgaaaactat ttcaagcgat agtaaagctc acggtacgac ttcgccgcga cctgccagct
     2581 aaaatccatt gccatagcct gacgttgcac aaaccgccac agtgaaggac gggaccacag
     2641 tacaaaagca cgtcgaatag cccgtaacag cgaccaggca ttactatctt caaagacaaa
     2701 cccactggcg acgccatctg caaggttctc aagagaacag tcagaaaccg tatcagcaag
     2761 cccaccggtg cgccgcacta acggcagcgt accgtacttc aatccataaa gttgcgttaa
     2821 gccgcacggt tcaaaacggc tgggcaccag aatgacgtcc gcgccgccca taatgcgatg
     2881 cgaaaatgct tcgtgatagc caatctgaac gcccacctgg ccggggtatt ccgctgccgc
     2941 cgcaaggaaa ccttcctgca gcaccggatc gcccgcgccg agtagcgcca gctgcccgcc
     3001 ctgctccaga agaccaggta aggcttccag caccaggtcg agacctttct ggctggtaag
     3061 acggctcacc actgcaaaaa gcggcacttt atcgtcaacc ttaagcccca ttgcgatttg
     3121 taactggcgc ttattttccg ctttatcttc caacgtatcg cgggtgtaac gcgaggccaa
     3181 cagtaagtcc gtctctggac tccagatttt ctcgtccacg ccgttcagta cgccggaaag
     3241 acgcccttca cggtgacgct gttgcaacag accttccata ccgtaggcaa actgcggttc
     3301 ggtgatctcg cgagcgtagg ttgggctgac tgccgtaatg tgatcggcat agtacagacc
     3361 ggccttcagg aaagagattt gtccgttgaa ttccagccca tgaatattaa agaatgacca
     3421 tggcaattgg atgtcattca tgtgatgtgc ataaaacatg ccttgatagg ccaggttgtg
     3481 cacagtaaac accgacttcg ccggacgccc gcgcgccgcc agatacgcag gcgcaaggcc
     3541 tgcatgccag tcgtgcgcat gcaccacatc aggacgccag aatgggtcaa gcccgctggc
     3601 catttctgcc ccaacccacc ccagcagcgc aaaacgcaat acgttgtcgg tataggcaaa
     3661 taagttggta tcgtgatacg ggcttcccgg acgatcatag agatgcggcg cgtcaatcag
     3721 gtatatgcca accccgttgt aatgaccgaa caaaagcgtg atatgtccgg cgaaggtatc
     3781 acgacgggat actacctgcg catcggtcac gccacggcga atatcgggaa atgcaggcaa
     3841 cagtacgcga gcgtcaacgc cgtctgcgat ttgtgctgcg ggtaatgccc caataacatc
     3901 agccagaccg ccggttttaa gcagcgggaa catctctgaa catacatgta aaacctgcat
     3961 tatcgctcct gtttatgccc taacttccgt agcatttcgc gcgttaccag cacgatgcct
     4021 tcttctgaac gatagaaacg acgtgcatct tcctctgcgt tttcaccaat caccatgcct
     4081 tccggaataa cacaagcacg atcgatgacg cagcggcgca gacggcacga gcgacctacc
     4141 catacttccg gtaacaatac ggcggaatca atgttgcaga atgaattcac gcgaacgcgc
     4201 gagaacagaa cggactgcac caccaccgaa ccggagatca cacaaccgcc ggataccagt
     4261 gagttaaggg tcatcccgtg gctaccggag cgatcctgca cgaatttcgc tggcggtaat
     4321 gattcattgt aggtgcgaat tggccaattg cgatcgtaca tatccagttc cggcaccaca
     4381 gaggccagat cgaggttcgc tttccagtaa gcttccagcg tacccacatc gcgccagtac
     4441 ggctcggcat ccgggtcgga ttgtacgcaa gagagcggga acgggtgcgc ataggccaga
     4501 ccggcttcgg tgatcttggg aatcaaatct ttgccaaagt cgtggctgga gttctcatcg
     4561 cgatcgtctt cttccagcag ttcatacaga tagtcggcgt caaagacgta aatacccata
     4621 ctcgccagag atttgctcgg atcgttcggc attgacggcg ggttagctgg tttttcaacg
     4681 aattcgataa ttttatcgtt ctcatcaacc gccataacgc caaacgcgga ggcttcttca
     4741 atcggtactg gcatacaagc aacggtgcaa cgtgcgcctt tttcgacgtg gtcgataagc
     4801 atacgcgagt agtcttgctt gtagatatgg tcgcccgcca ggatcaccac gtattccgct
     4861 ttataacggc ggataatgtc gaggttttgg gtgaccgcat ctgcggtgcc gcgataccag
     4921 ttttcccctt tcattctctg ctgtgctggc agcagatcga caaactcgtt catttcttca
     4981 ttgaagaatg accagccgcg ctggatgtgc tgcaccagag tgtgggactg gtactgggtg
     5041 atcacgccca tacgacggat cccggagttg atgcagttag acagcgcaaa gtcgataatg
     5101 cggaacttac cgccgaagtg tacggccggt tttgctcgct tattggttaa atccttcagg
     5161 cgggtaccac gtcctcccgc cagtatcagg gcaacagatt tcaatggcag ctggcgcgcc
     5221 aacattaagt gatcgttctt ctctaaacta accatgacta actccttttt tatcatctct
     5281 ggaacacaca caatccgtgt gcaggtccct gccagacagc cgtaatcact gggttatcct
     5341 ctccagcgaa tgggggaatg gcgtgccact ccccagcagg taaaacaatc tctgttacct
     5401 caagcgtggc attaattgcg atcaaaaagc gatccgagag cagaatttgc agctgtttcg
     5461 gcccgttttg ccactcatcc gtgcttaaag gttgagcata tcgatttagc caacggacat
     5521 tgccgtcgcc ttcttcccac cagcgattct ccatcaaagc gggaatgcgc ttgcgcagat
     5581 ggattaacgc ggcggtaaat gcggttaaac cactgcttgc ctgcgaccag tccaaccagg
     5641 ttaattggtt atcctgacag taggcattgt tattgccatg ctggctgtga ccatgttcgt
     5701 caccggccag taacatcggc gtaccctggg agagcaacaa cgttgttaac agggcgtgaa
     5761 tgctgtcgcg ccgccgttca accagatcaa gagtaccgcc taacccttct ttaccatgat
     5821 tgttactgta attgttgttg gtcccgtcgc gattttcttc tccgtttgct tcattgtgtt
     5881 tatggttgaa gcaaacgcag tcgcgaagcg taaaaccgtc atgcgcggtg acgagattaa
     5941 tcgcggcact cggcagacga tcattacgtt taaaaacatc gctggaggca gcaaaacgcc
     6001 cggcaaacgc tcccagaggt aaatcataat gcagccagaa acgacgggct gcatcgcgga
     6061 aatgatcgtt ccactcggca aacagcggcg ggaaatttcc cacctgataa ccaccaggag
     6121 cgatatccca cggttcagca attaacttca cctgcgagag caccgggcag ttctggatag
     6181 cggtaaacaa cggcgcatcc tgacgaaact ctggcgtacg gcccatgatt gccgccagat
     6241 caaagcggaa accatcgacg tggcaggttt ctacccaata acgcaggcag gcgctggcat
     6301 aatctaccac cgccggatga ctcaaattga gcgtgttgcc acaaccggtc cagttgtgat
     6361 aatcgccgtc ttctcttatc caataatagc tacggttgtc gatcccacgc agcgagaata
     6421 acgggccgtc gaggtccagt tccgcactat ggttgagcac gatatcaaga atgacttcga
     6481 tacccgcttt atgcagtgct ttgattgcat cgcgaaactc atccagcgcc gtttctggcg
     6541 agcaggcata cgccggatgc agcgcaaaca tcgccaccgg gttgtaaccc cagtagttac
     6601 taagccccat acgttgcaga cgtggttcac tggcaaattg tgccaccggc agcagttcca
     6661 gcgcggtaat gcccaactgt ttcaaatagt tgatcatcac cggatgcccc agggctttat
     6721 aagtgccacg gatctcgacc ggaatctccg ggtgcaggta cgttaatcct ttgacatggg
     6781 cttcataaat gatggtgctg ccccacggcg tgcgcggcgg ggcatcatcc tcccagtcat
     6841 agtgatcaac caccactacg catttcggcg caatggcggc gttgtcgcga tagtcaggtt
     6901 cattatgacc ggcgtgcagc agcgggttat ctttaaactc cccgtcaatt tgccgcgcgc
     6961 aaggatcaat caacaacttc gccgggttaa agcgatgccc ctcggcaggc tgccaggggc
     7021 catgaacgcg ataaccataa cgcaaacccg ggcgcgcatc cggcagataa ccgtgccaaa
     7081 tgtcgccact gtgccctggc aagtcatagc gatgttcctg gccattggca tcaaagacac
     7141 acagctctac ccgctcggca tgagcggaga aaagtgtgaa gttgacgccc tgaccgtcgt
     7201 aatgcgcgcc gaggggagtg ggtttgccaa tggcgagttg tgtcattctg cctcccgaac
     7261 cagccagata gtggccagag gtggtagcgt caggcccagt gaatgctgac gaccgtggct
     7321 ggcaatctca tcgctgtgta ctgcgccgcc attgcctgca ttactgccgt gatagtgcat
     7381 ggaatcggta ttgaggattt cacgccattt acccggctgg ttaataccga agcgataatc
     7441 atgacgcggc accggtgtaa agttactggc aacgatgatt tcgttaccct ctttatcgcg
     7501 acgcacaaag atcagcaccg agcgttcttt gtcatccacc accagccatt caaagccgta
     7561 cgggtcaaaa tccagttcat gcattgcttt atggtggcgg taggtgtggt tcagatcgcg
     7621 caccagacgc tggacaccgt ggtgccagtt atcgccgcct tccaacagat gccagtcgag
     7681 gctggcgtca tggttccact cgcggccctg ggcaaattcg ttccccatga acagtagttt
     7741 cttgcccggg aatgcccaca tccagccgta gtaggcgcgc aggttagcga atttctgcca
     7801 tgcgtcgccc ggcatccggt cgagcatcga ttttttaccg tggaccactt catcatgcga
     7861 caacggcagg acgaagtttt cagtgtagtt gtagagcatc ccgaaggtca gtttatcgtg
     7921 atgatactga cgataaatcg ggtcgagctt catgtagtcc agggtgtcat gcatccagcc
     7981 gaggttccac ttgtaccaga agcccagacc gcccatatcc tgcggacgag aaacgccagg
     8041 gaaatcggta gactcctccg ccattgtcac tgcaccggaa acctgctcac caagaatacg
     8101 gttggtatta cgcaagaatt caatcgcttc aagattctcg cgaccgccaa attcgttcgg
     8161 gatccactcc ccctctttac ggctgtagtc gcgataaatc attgacgcca ccgcatcgac
     8221 gcgcagcgca tcaataccaa aacgttcaat ccagtaaagt gcgttaccga caaggaagtt
     8281 actgacttcg cggcgaccat agttgtagat cagcgtgttc cagtcctgat gatagccttc
     8341 gcgcggatcg ctgtgttcat acaagttcgt gccatcaaat tcggcaagcg caaagtcatc
     8401 ggtcgggaag tggcctggca cccagtcgag aatcacgttc agaccagctg cgtgtgcggc
     8461 atcaatgaaa taacggaagt cgtcgcgagt accaaaacgg cgggtcggtg catacaggcc
     8521 ggttggctga taaccccaac tgccatcgaa gggatgctcg ttaatgggca gtagctcgag
     8581 atgggtaaag cccatccatt tagcataagg caccagttga tcggccagct cgcggtagct
     8641 caaccagaaa ttgttgtcgg tgtggcgacg ccaggagccc aggtgaactt cataaataga
     8701 gattggcgca tcaaactgat tcgctttttt gcgctcttca gtctgcacaa ccttttccgg
     8761 cagcccgcaa ataagagacg cggtttccgg gcgcatttgc gcttcgaagg cataagggtc
     8821 ggacttcaga cgcaagttgc cattggcatc aatcatctcg tatttataga gctgaccgtt
     8881 atgcgccccg gggataaaca gttcccagat tccgctctct ttacgcagtc gcatcggatg
     8941 acggcgacca tcccagtagt tgaattgccc aaccaccgag acccgacggg cgtttggagc
     9001 ccagacagaa aaacgcgtac ctgtgacgcc atccatagta tctgcatgcg cgcctaaggt
     9061 ttcatacgga cgtaggtgag taccttcaga taatagccag gcatccattt cctggattag
     9121 cgggccaaaa cggtaaggat catcaatcag gttttgctga ccatgccaga caacagccaa
     9181 ctgatagcgg aaaaaattct tacgtcgcgg aatgacaccg ctaaagaatc cccgtgagtc
     9241 gagacactcc agttttgcga gtttgcgccc ggttttcggt tcaatcaccc acacatcggt
     9301 agcgtcgggt aaaagggcac ggacttccag tcccgcggtg gttttatgca tccccagtac
     9361 ggaaaaagga tccgcaaaat ggcctgcaat tagcgcgtta atcacgtctc tatcgatacg
     9421 atcggacatg cttgtcttcc tgttttattg tgtcacccca tccaactgaa tttttgactt
     9481 ctggttgtga catttttttg acctgaacgg cgcagcactc tgtgcatcct ctctgcgtcg
     9541 tcctcacttc aggtaaggct gtgaatactc gtgtattccg ccacccttaa agaatagcca
     9601 atgctctatt taactcccgg taaatcatga aacatctgcg cttactcctg tattacgcac
     9661 tagcaggggc ggcatcgcgc cccagattta atgaataaag attacgccag ttgacgaagc
     9721 atccgacgca gcggctccgc ggccccccac agcagctggt cgcccacggt aaaggctgac
     9781 aggaactccg gtcccatatt cagcttacgc aggcggccta ccggcgtggt cagcgtgccg
     9841 gtaacggcac ctggggttag ctcacgcata gtgatttccc gatcgttcgg aacaactttc
     9901 gcccacggat tgtgcgcagc cagcagttct tccacggtcg gaatagacac atcttttttc
     9961 aatttaatag tgaatgcctg gctgtggcag cgcaatgccc cgacacgcac acataaacca
    10021 tctaccggaa ttacggaaga tgtgttgagg atcttgttgg tttccgcctg ccctttccac
    10081 tcttcgcggc tctgaccgtt atcaagctgt ttgtcgatcc acggaatcag gctacccgcc
    10141 agcggcacgc caaagttatc taccggcagc tcaccgctac gggttaaggt tgtgactttg
    10201 cgttcgatat cgagaatagc agaggacggg ttcgcgagtt catctgccac atggccatac
    10261 agatggccca tttgggttaa taactcacgc atatgtcgcg caccaccgcc ggaagcggcc
    10321 tggtaggttg caacggacac ccaatcaaca agatcattgg cgaataaacc ccccagcgac
    10381 atcaacatca ggcttacggt acagttaccg ccaacaaaag tcctgatgcc attatttaat
    10441 ccgtcggtaa tgacgtcctg attgacgggg tcaagaatga tgatggcgtc atctttcatg
    10501 cgcagagatg atgctgcgtc aatccagtaa ccttgccatc cgctttcacg aagctttgga
    10561 tagatttcgt tggtataatc gccgccctga caggtcacaa tgatatcgag ggcctttagc
    10621 gcctccagat caaaggcatc ctgaagtgtg ccagtggttc cgccaaaaga cggcgcagcc
    10681 tgaccaagct gagaagtaga aaagaagaca gggcgaatgg cgtcgaagtc gcgctcttca
    10741 accatgcgtt gcatgagaac ggagccgacc ataccgcgcc agccgataaa accaacattt
    10801 ttcataagcg ttttttcctg caaagatgtg tgctgtataa atgtgccggt ctcctcctgg
    10861 cacatctttc accatacaaa aagcagccaa agtcgcaagt gaaattaatc aatgatagcg
    10921 aagccatcag taatgcgact tatcctgctt tgttagcacg cagaaagtcc gcggcaatta
    10981 tcagggaatt tgagttatga atgaaatcat ttctgcaaca gttttattga tcctgattat
    11041 ggatccgctc ggaaacctac ctattttcat gtccgtactg aaacatactg aaccgaaaag
    11101 acggcgggca atcatggtgc gagagttgct tattgctctc cttgtgatgc tggtgttcct
    11161 gtttgcgggc gagaaaattc tggcatttct tagcctgcgg gcagaaaccg tctccatttc
    11221 tggcggcatc attctgtttc tgatcgccat taagatgatt ttccccagcg cttcaggaaa
    11281 tagcagcggg cttccggcag gtgaagagcc atttatcgtg ccgttggcaa ttccgctggt
    11341 cgccgggccg actattctcg ccacgctgat gttgttgtct catcagtacc cgaatcagat
    11401 gggacatctg gtaattgctc tgctactggc ctggggcggc acctttgtca tcctgctaca
    11461 gtcttcgcta tttttacgtc tgctgggcga gaaaggggtg aacgcacttg aacgcctgat
    11521 gggattgatt ctggtgatga tggcaactca gatgttcctc gacggcattc gaatgtggat
    11581 gaaggggtaa ggttcaaaca ggtgttgcac acaaccagac gcggcacctg ttttattacc
    11641 tgaactgata atgaccttcg cccaatcgga taacccttcc ctcttcttct gcgcggttca
    11701 gccacacact aatttgttcg atggttaaac cagtacaaga cgccagcctt tcaatactta
    11761 tcgattcctt tgcaaggatc gccagttccg ccacaaagag ttgataaaaa tcgacagggg
    11821 tatgcgcggg cattactgcg taatttgcgt catcttcgtg cagcgaaaac agttctggtt
    11881 gagcgttacg gagtgctctg tcatacatca gcccaggaac gtctggcagt ctgatcgtca
    11941 cattctcagc ctgttcagca ctccatgacg ccccattttc tactaatcgt gaattggctg
    12001 attgcatatc ctgatttggt ttaacctgca taggcagcca ctgatgtttt aatgcctcca
    12061 tcgccccggt tatcgttcca ccggtcattc ccgcacgtac aaccattgcg ctttcagcaa
    12121 ggcaataaat atatttattc cgcgccatcg cattgccgac ggtgaaacgt acctctgggt
    12181 aaaacggcga aattaacacc aagttgcctg ctataagccc ttcacgccat ttcactaacg
    12241 tactcgtttt taacaagcta tcagctaata cgccaacggc agttcccccg acctccagaa
    12301 ccgaggccat tgcacattca tcaatacctc gcgcaccacc agagataacg caaatcccct
    12361 gttgggccag tttcgcggcc agttgttggg tatagcgcaa atcgtcagtc ggagcatctc
    12421 gcgagccaac aatcgccata ccttccgctt gcagtaatgc tttattcccg cagccaaata
    12481 aaacgggagg tgcatccgtt cgcaatcggt ttttcaagcg aacgggataa tcggcatctc
    12541 cgcgggttaa gatccataaa ccggcgcgtt gccacttatc taccgccaac gccagactat
    12601 gaccacgcgc cagcaagcca agaatacgct cctgagaaat acgcggatcg ctccagcctg
    12661 taagtttctc ttgcggttgc ggcatcagaa gctcggcggg actgatacgt tggtgcttca
    12721 gccataatgc aaacttcccc cattcactat tactaagagg tttatactca ctcgccgccg
    12781 cgcgagaaaa atcgctggtt agcaggagag ttgcttgtgc attggctgaa agattcatca
    12841 attttttacc gaggtagaag caagggcaag aggataaacc gtcgggcaac ctgcctggcg
    12901 taacagtgct gtcagaactg tcagcgtcca tgcagaatcc acgatatcat caaccagcaa
    12961 cgccggacct ggcatcaaag gaggggtaat cacaaacgcc ccgtcgagat tttgacactg
    13021 gtgaaaacgg ttttgctgca ttttctgcgg tggattgtcc acgacttttt caatggcatc
    13081 aataaaaggt aagccaagtt tcgccgccag tcgccgggca aaatcaggaa ccaggtcgag
    13141 gtgccttaat gaaggaacgc agcataccca ggtgggttca ggatgcggat tccagcgttg
    13201 gcgaaccatt tccgcacatg cctccaccag ttcatcactg aagcgccccg tttttttctc
    13261 ttgggctacc tgctttcccc acccggaatc tccccaacga gaaaggattc gccccgtaga
    13321 tccttgtaaa cccgcaggca agttcccttt aaatccgtat tgcgtaaaag ccccagatgc
    13381 cacctgttta ttgagattta atggcaggtc agcgtgttta ataaataaat ttgccgcatg
    13441 gagtaagccg ctatcaatgt cgggcgacaa cagcggatgc tgaagacaac tgctacattt
    13501 accacaacgt tcggcacttg gctcatcgag tgcgcggcgc agaaattgca ttttgcactc
    13561 cttggtcgtg atatagcttt caacgtctgc caactcactt tttctctgat tcatcaaatg
    13621 gttaattcgt tcatgatcag gagaaaaact gacgatagtt ctgcgccata atttctcggt
    13681 atacaccaca ggcgatggat tttcagctac caataatttc agtgcttttt ctatttgccc
    13741 ataacgaaga ttcgtccgtt gttcaatgcc tcgtagggta agaccgtcat tctcgctaag
    13801 tacgttgagt atttcatgaa tttgcgcctc cgcaggaaag gcgctttcac ggaagaattg
    13861 atgaatagcg cggtcttcac caccacaaag caatatgcca actgcggaat ctatagcacg
    13921 cccggcacgc cccacctgtt ggtagtaacc gacaatagac cccggcatct gataatgaat
    13981 gacgaaacct aaatcaggtt tatcaaatcc catacctaat gccgtcgtcg cgaccagcac
    14041 tttgatttta tttgccagca gtgcctgctc cagatactcc ctggcagtat tagaatcttc
    14101 cgcaccttca caagtcacgc cgctgtaata agcaaatgca ctgataccat ttttccttaa
    14161 ccagtcggcg acaagttcag catcgcgagt cgtcagggta taaacaattc ccgatttgga
    14221 aaactgaggg ataaccgttg ctaaccatgc cagacgggat gactgttctc ctaagactaa
    14281 ggcatcaagc gccaaacttt cgcgagccag cgttccacgc tgaatcacaa tgtcacccaa
    14341 ttgctgacgg atatcctcaa cgacacggtt attcgctgtc gcggttgtac ccagaatagg
    14401 ggtattcgca ggcagttggc gcaaaatatc taatatacgt cggtaatccg ggcgaaaatc
    14461 gtggccccaa tcagagatgc agtgcgcctc atcgaccacc agtaaaccga tgcggtcagc
    14521 gactggatat aaaaccgttt caagaaaatc ctgattcgcc aaccgttcag gtgaaatcag
    14581 cagacaatct acgccacctt gcagcaactt atcgctaatg cgctgccatt cttccctgtt
    14641 tgtagaattc aacgtttcag ccgtaatacc taaccgttca gcggcggcaa cctggttacg
    14701 catcagggca agcaaaggag agatgataat tgtcgggcca gcgccgcgat cgcggaagat
    14761 tttactggcg atgaagtaca cggcactttt cccccagccg gtgcgctgta caaccagtag
    14821 ttttcgccgc tgattaacca ccgcatctat agcttcccat tgcccttccc tgaaagtagc
    14881 gctggtgtta cttaacatcc gctgtaacag caattcagct ccgtgtttct ccatcatctt
    14941 ctccctcgtc cgttaacaca gtgattagaa cggataatgt tccggtcttt ttgcgagaga
    15001 gatcaaaaag atgaagacag gagaaaagag aaatgaatta ctgcgagcaa gctcgcagta
    15061 atttgtcgca gtaattaact caacagctgg aacgcaatca tcccaacgat ggcaccgaca
    15121 gtgccgagga tggtttccat catggtccag gttttaagcg tttcggcttc ggtcgcgccg
    15181 gtaaatttac cgaacaacca gaaacccgcg tcgttaacgt ggctgacaac aatcgaacca
    15241 ccggcgatac aaatcgacag cgccgccatt tgcgcaccgg agtagttcag ttgttcaatg
    15301 accggcatca ccagtcccac cgccgttaaa caggcaacgg tagcagaacc ctgaatgatg
    15361 cgcactgcag ctgccagcac gaagcaggtg atggcaatcg gcaggcccat gccggttaac
    15421 gcttcgccca gtgccggacc gacaccagag tcaaccagca cctgtttgaa cacgccgccc
    15481 gcaccaatca ccagcagaat gatccccgcc ggttgcagcg cgtgaccgca aatctccatc
    15541 actttgtctt ttggcatacc ctgacgcatt gccagaccgt aaatcgccac caggcaagca
    15601 accagaatcg cggtaaacgg atggccgata aactcgaacc attcgtaagc agtagagcct
    15661 tctggcacaa aacgcgcggc aatggttttc agccccacca gcaccagtgg caacaggatc
    15721 agcgacaggc tgaatccgaa ggacggcatt ttgccttcgc cgagatgcgg ttcgctgatg
    15781 tcgtcaggaa tatgcaactc cacgtagcgg ctgatgaaat taccccacag cggcccggca
    15841 ataatcattc ccggaattgc cgcgcacagg ccaatcagga tcatccagcc aaaatcggca
    15901 ttcatctgcg atgccagcag cattggcgct ggcccaggca ccaggaacgc agccgctgcc
    15961 gccacgccgg cgaataatgg gattaccagc ttcaccaggt tcgtgccggt gtggcgcgcc
    16021 attgagaaag caacgctaat cagcagaaca atcgccactt caaagaacag cggtagcgca
    16081 caaaccagcc ccgcaaggcc gatggcataa tgcacgcggc tgtgaccgaa ggatttgagc
    16141 attttgacgg caatctgatc gaccgcgccg gtttcatgta agatcttacc aaacatggct
    16201 cccagagcga caaccaccgc caggaagccg agggtgcctc ccatcccttt ttccatcgtc
    16261 gctgcgattt tatcgagcgg cataccggaa aaaaggccag cccccatgga caccaccatt
    16321 aaagccagga aagcgtgcat acgcgccttc atgactaaaa acagcagcag taaaacagac
    16381 cctactgctg ttaaaacaag cgttaatgta gtcactactt atttgccttt tttaataacc
    16441 tcaatggtgc ttgccacaac accttccagc ggttgatcaa tatccaccac cagtacatcg
    16501 gtttcgtccg cacccggctc ctgcagcgtt tcaaactgcg tcaccagcat ttgggtttta
    16561 aagaaatggc ctttgcgcgc tttcaggcgg ctttcaatca catcaaaatc gcctttcagg
    16621 tagatgaaag agagattagg attaccttca cgcagcaagt cgcgatagtg ttttttcagt
    16681 gcagaacaga cgatcagcga tactttattg gtgcgctgca tagcaaacgc ggcgtcgttc
    16741 agcgcctgca accacggttt gcgatcgtcg tcattcagcg gttcgccaga cgccattttt
    16801 tcgatattgc agcgcggatg gaggaaatcg ccatcaagaa acgcggcatg aagttgatgc
    16861 gccacttcac tggcgaccgc agatttgccg ctgcccgata cgcccatcaa gacgtaaatg
    16921 tggtgatcat ggttagtcgt gctcaaagtg gtgcccccac aatacaagaa ttaaaaatgt
    16981 tacgggtaac tgttatcggt aacattgtcc agccggacaa tatcagaagc aatatccatc
    17041 cgtgccttaa gtgtataagt gtgagctact tcaaatttgt aggcttaaat agatccgccc
    17101 ggtgacaagg tgaaacctaa atctaacatt ttcggtgtca cagattcgcc acgaatacgc
    17161 gccagcaggc gctcagcgcc aatgctgccc atccgctcac gcggcgtcag cacactcgct
    17221 agccgtggct ccatcacctg accaatgtca tgaccgtgga aaccggcaat cgccatatcg
    17281 tcaggaactt tcaaccccag acgctgacat tcaaacgccg cgccgaccgc caggtcatca
    17341 ttagtacaga acacgccatc cagctgcgga tattcccgcc gcgcctggcg aatcagttca
    17401 ataccggaag agtaagaaga agattgctca accatcacgc tatacggcac caggcctgca
    17461 tccagcatcg cctgttcgta tcccttctgt ttgatgatag tacgttcgtc gaggcgtgcg
    17521 ccgagatagg caatgtgacg atgcccgcga gcaataatgg cggtggtcat ctggcgtgcc
    17581 gcttcaaagt tatcaaaacc gacggcgata tcgaggcacg gcgacttact gtccatcaac
    17641 tccaccaccg gaatccccgc cacttcaatc atctttaagg tacgcggcgt gtgggtacgt
    17701 tcggtgagga tcaggccgtc gatattccat gagagcattg attcgaggcg ttcttgctcc
    17761 atttccggtt tataaccgta gtgcgccagc atggtttgat aaccgtgcgc gtcggtgacg
    17821 ctttcgattc cgcgtaatac ttccgcgaaa acctggttgg tgagagaagg taacaggacg
    17881 ccaatcgccc ggctggtggc gttagagagt atatcgggcg cgcgattggg aatataaccc
    17941 agttcatcaa gagcggcggc aatcttgccg cgtagagcga cggaaacctg ctccgggttg
    18001 cgtaaaaaac ggctgaccgt cattttggtc acgcctacac ggtcagccac atcctgaagt
    18061 acgggtcttt tctttttcat cgtcctgaag gtacaaaaga gatagattct cttagtttaa
    18121 cacggacgca tcacaacctt cccgattcct caggaaggtt tgtgagataa atttagactg
    18181 gcggtaaatc aaacagcagg atttcactgt cgctatcagc atggatggaa atcgcctgct
    18241 catcccagat tgccagacca tcgctggtcg aggctttcac gccgttaatg gtgacattgc
    18301 ctttcaccac ctggatccag acgcggcgtt cagcggcaat ctgatgcacc gactgctcat
    18361 ctttcaccag cgcccaacga tacagttcca tatcctgatg cactttcaat gaaccatcac
    18421 gcgcatccgg cgaaagcacc agttgtttgc cctgcacggc atcgaagcga cgctgttcat
    18481 aacgcggcgt aatgccgttt tcttcaggca tgatccagat ctgatacaga tgcagacgct
    18541 cggtgctgct tgggttgtac tctgagtgac gaatacccgt acccgcactc ataatctgga
    18601 actcgcccgc cggaacctgc tctttattgc ccatgctgtc ctggtgctca acagtacctt
    18661 ccagcacgta ggtcaaaatt tccatatctt tatgcgggtg agtgccgaag ccctgccctg
    18721 cttcaatcac gtcgtcgtta atcacgcgca gcgcagagaa gcccataaag ttcggatcgt
    18781 aatagttggc aaaagagaaa gtatgccagg agtccagcca gccatgattt gcatgaccgc
    18841 gttcatttgc tttgcgtaag tagatcattt gtattcgccc cctgaatgat ttcgatgggc
    18901 ttagtgtgga cccgatccgc ctgggatgat agagggtgaa aattgacccc tctgttcaaa
    18961 aaaattgaac tattcgaggg gccatcaaac ttacctcgcg agagtcactg cggagggaga
    19021 agcttgctca aaaccgcgtt caaggatttc cagattcgta agaacttcag attccttgac
    19081 gtaatttggc gcaccgttgg tgatggtttg atacaacgca tcataaacgc gcccataatc
    19141 gcccatctct ggcttcatct cttctcttac cgtcacgccc tcgtcattga catactccag
    19201 cacaccgacc gaatcatccg ctgcgaatcc tggttcgccc ggcataatat tagccttcag
    19261 gctggtttcc tgctggtcga taccgtattt aataaacgaa cctttcttac cgtgaacgat
    19321 aaatttcgga taatcgattt tcaccagatg gctggttttg acgatggctt ttagatcgcc
    19381 ataaaacagc tgcgcttcaa aggtgtcgtc cggattggct ttattacgca ggctgcggat
    19441 gtcataagcg acgtgatccg ggcgaccgaa cagagaaata atctggtcca tcgtatgcac
    19501 accaaggcca tagaacgcgc catcctgcgg tagccccggt ttggtttctg ccaccgggcg
    19561 gtaatagtca aaatggcttt ccacttcgac aatctctccc agcttgccgc tttcaatcgc
    19621 ttttttcgct gtcaggaagc aggaatcaaa gcgacgattc tgatacggcg taacgatcag
    19681 ccctttgctt ttcgccagtg caaacagctc tttcgcctgc gcaattgtcg gagtgaacgg
    19741 tttttcgacc agcacatttt tcccggcttc cagcgcgcgc ttcgcgtact cgaagtggct
    19801 gtccgcgtgg gtgcagacga caaccagctt aacatcggga tcgtttagca cttcgtcgag
    19861 atcgctggtg aaatggatat gggaataaat gggggcctgt tcttccggct tcgcatggcg
    19921 gcgaaaaata tgcgcgacat gccagctatc cttgcggtta agtacatacg gcagatggta
    19981 acgggtggtg cttttgccga agccaataaa ggcgcagtta atgaccatga tttcgtcctt
    20041 tttaaggtgg ttatttacac cttagcgcaa agcggacgtg gttcctactg gagtgcgcat
    20101 aacgaacaca agcacttccc gaggataaat tgagaacgaa aggtcaaaaa aaaagccagc
    20161 acccggctgg ctaagtaata ctggaagcaa tgtgagcaat gtcgtgcttt caggttctcc
    20221 gcgagggtct tcctgatcgc gagacaataa taatcattct cattcgcact tgtccaacac
    20281 tttttgcaaa aaaatgcatt tgactcgcat ttgaaagtca atgatgttga aagggacatt
    20341 taccccaaag aggacaaagg aatgagtgag atagtaatac gccacgcaga aacacgcgat
    20401 cacgaagcca tcaggcagat tcacgcccag ccggaggtgt attacaacac gctacaggtg
    20461 cctcatcctt cagaacaaat gtggctggaa cgactcaccg ctcgccctgg catcaagcag
    20521 ttagtcgcct gtattgatgg aatcgtcgta ggccatctca ccattgacgt gcaacaacgc
    20581 ccacgccgca gtcatgttgc cgattttggt atctgtgtcg actcccgttg gaagaaccgc
    20641 ggcgtcgcca gcgccctgat gcgagagatg attgaaatgt gcgacaactg gttgcgggta
    20701 gatcgtattg agctaacggt gttcgtcgat aacgcgccag caattaaggt ctataaaaaa
    20761 tttggctttg agattgaagg gactggcaag aagtacgcgc tgcgtaatgg tgaatatgtc
    20821 gatgcgtatt atatggcgcg ggtgaagtaa gatagtgccc tttttctgag atgga
//
LOCUS       DADSYM010000084        20834 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2415
            SAMN08039579-rid11723173.denovo.084, whole genome shotgun sequence.
ACCESSION   DADSYM010000084 DADSYM010000000
VERSION     DADSYM010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039579
            Sequence Read Archive: SRR6362533
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 20834)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20834)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980162.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 03:39:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,484
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 4,347
            CDSs (with protein)               :: 4,347
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 43 of 85
            Pseudo Genes (incomplete)         :: 37 of 85
            Pseudo Genes (internal stop)      :: 19 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20834
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039579-rid11723173.denovo.084"
                     /strain="LGE2415"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8302 N 95.9628 W"
                     /collection_date="2015-08-24"
     gene            308..4768
                     /gene="gltB"
                     /locus_tag="J1Y83_002649"
     CDS             308..4768
                     /gene="gltB"
                     /locus_tag="J1Y83_002649"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase large subunit"
                     /protein_id="HBA7630788.1"
                     /translation="MLYDKSLERDNCGFGLIAHIEGEPSHKVVRTAIHALARMQHRGA
                     ILADGKTGDGCGLLLQKPDRFFRIVAQERGWRLAKNYAVGMLFLNKDPELAAAARRIV
                     EEELQRETLSIVGWRDVPTNEGVLGEIALSSLPRIEQIFVNAPAGWRPRDMERRLFIA
                     RRRIEKRLEADKDFYVCSLSNLVNIYKGLCMPADLPRFYLDLADLRLESAICLFHQRF
                     STNTVPRWPLAQPFRYLAHNGEINTITGNRQWARARTYKFQTPLIPDLHDAAPFVNET
                     GSDSSSMDNMLELLLAGGMDIIRAMRLLVPPAWQNNPDMDPELRAFFDFNSMHMEPWD
                     GPAGIVMSDGRFAACNLDRNGLRPARYVITKDKLITCASEVGIWDYQPDEVVEKGRVG
                     PGELMVIDTRSGRILHSAETDDDLKSRHPYKEWMEKNVRRLVPFEDLPDEEVGSRELD
                     DDTLASYQKQFNYSAEELDSVIRVLGENGQEAVGSMGDDTPFAVLSSQPRIIYDYFRQ
                     QFAQVTNPPIDPLREAHVMSLATSIGREMNVFCEAEGQAHRLSFKSPILLYSDFKQLT
                     TMKEDHYRADTLDITFDVTKTTLEATVKELCDKAEKMVRSGTVLLVLSDRNIAKDRLP
                     VPAPMAVGAIQTRLVEQSLRCDANIIVETASARDPHHFAVLLGFGATAIYPYLAYETL
                     GRLVDTHAIAKDYRTVMLNYRNGINKGLYKIMSKMGISTIASYRCSKLFEAVGLHDDV
                     VGLCFQGAVSRIGGASFEDFQQDLLNLSKRAWLARKPISQGGLLKYVHGGEYHAYNPD
                     VVRTLQQAVQSGEYSDYQEYAKLVNERPATTLRDLLAITPGENAVNIADVEPASELFK
                     RFDTAAMSIGALSPEAHEALAEAMNSIGGNSNSGEGGEDPARYGTNKVSRIKQVASGR
                     FGVTPAYLVNADVIQIKVAQGAKPGEGGQLPGDKVTPYIAKLRYSVPGVTLISPPPHH
                     DIYSIEDLAQLIFDLKQVNPKAMISVKLVSEPGVGTIATGVAKAYADLITIAGYDGGT
                     GASPLSSVKYAGCPWELGLVETQQALVANGLRHKIRLQVDGGLKTGVDIIKAAILGAE
                     SFGFGTGPMVALGCKYLRICHLNNCATGVATQDDKLRKNHYHGLPFKVTNYFEFIARE
                     TRELMAQLGVTRLVDLIGRTDLLKELDGFTAKQQKLALSKLLETAEPHPGKALYCTEN
                     NPPFDNGLLNAQLLQQAKPFVDERQSKTFWFDIRNTDRSVGASLSGYIAQTHGDQGLA
                     ADPIKAYFNGTAGQSFGVWNAGGVELYLTGDANDYVGKGMAGGLIAIRPPVGSAFRSH
                     EASIIGNTCLYGATGGRLYAAGRAGERFGVRNSGAITVVEGIGDNGCEYMTGGIVCIL
                     GKTGVNFGAGMTGGFAYVLDESGDFRKRVNPELVEVLSVDDLAIHEEHLRGLITEHVQ
                     HTGSQRGEEILANWSTFATKFALVKPKSSDVKALLGHRSRSAAELRVQAQ"
     gene            4781..6199
                     /locus_tag="J1Y83_002650"
     CDS             4781..6199
                     /locus_tag="J1Y83_002650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001540975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HBA7630789.1"
                     /translation="MSQNVYQFIDLQRVDPPKKPLKIRKIEFVEIYEPFSEGQAKAQA
                     DRCLSCGNPYCEWKCPVHNYIPNWLKLANEGRIFEAAELSHQTNTLPEVCGRVCPQDR
                     LCEGSCTLNDEFGAVTIGNIERYINDKAFEMGWRPDMSGVKQTGKKVAIIGAGPAGLA
                     CADVLTRNGVKAIVFDRHPEIGGLLTFGIPAFKLEKEVMTRRREIFTGMGIEFKLNTE
                     VGRDVQLDDLLSDYDAVFLGVGTYQSMRGGLENEDADGVYAALPFLIANTKQLMGFGE
                     TRDEPFVSMEGKRVMVLGGGDTAMDCVRTSVRQGAKHVTCAYRRDEENMPGSRREVKN
                     AREEGVEFKFNVQPLGIEVNGNGKVSGVKMVRTEMGEPDAKGRRRAEIVAGSEHIVPA
                     DAVIMAFGFRPHNMEWLAKHSVELDSQGRIIAPEDNDNAFQTSNPKIFAGGDIVRGSD
                     LVVTAIAEGRKAADGIMNWLEV"
     gene            complement(6267..6731)
                     /locus_tag="J1Y83_002651"
     CDS             complement(6267..6731)
                     /locus_tag="J1Y83_002651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF386 domain-containing protein"
                     /protein_id="HBA7630790.1"
                     /translation="MMMGEVQSLPSAGLHPALQDALTLALAARPQEKAPGRYELQGDN
                     IFMNVMTFNTQSPVEKKAELHEQYIDIQLLLNGEERILFGMAGTARQCEEFHHEDDYQ
                     LCSAIENEQAIILKPGMFAVFMPGEPHKPGCVVGEPGEIKKVVVKVKADLMA"
     gene            complement(6728..7603)
                     /locus_tag="J1Y83_002652"
     CDS             complement(6728..7603)
                     /locus_tag="J1Y83_002652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417689.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine kinase"
                     /protein_id="HBA7630791.1"
                     /translation="MTTLAIDIGGTKLAAALIGADGQIRDRRELPTPASQTPQALRDA
                     LSALVSPLQAHAQRVAIASTGIIRDGSLLALNPHNLGGLLHFPLVKTLEQLTNLPTIA
                     INDAQAAAWAEYQALDGDITDMVFITVSTGVGGGVVSGGKLLTGPGGLAGHIGHTLAD
                     PHGPVCGCGRTGCVEAIASGRGIAAAAQGELAGANAKTIFTRAGQGDEQAQQLIHRSA
                     RTLARLIADIKATTDCQCVVVGGSVGLAEGYLALVETYLAQEPAAFHVDLLAAHYRHD
                     AGLLGAALLAQGEIL"
     gene            complement(7600..8289)
                     /locus_tag="J1Y83_002653"
     CDS             complement(7600..8289)
                     /locus_tag="J1Y83_002653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HBA7630792.1"
                     /translation="MSLLAQLDQKIAANGGLIVSCQPVPDSPLDKPEIVAAMALAAEQ
                     AGAVAIRIEGVANLQATRAVVSVPIIGIVKRDLEDSPVRITAYIEDVDALAQAGADII
                     AIDGTDRPRPVPVETLLARIHHHGLLAMTDCSTPEDGLACQKLGAEIIGTTLSGYTTP
                     ETPEEPDLALVKTLSDAGCRVIAEGRYNTPAQAADAMRHGAWAVTVGSAITRLEHICQ
                     WYNTAMKKAVL"
     gene            complement(8337..9827)
                     /gene="nanT"
                     /locus_tag="J1Y83_002654"
     CDS             complement(8337..9827)
                     /gene="nanT"
                     /locus_tag="J1Y83_002654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312124.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialic acid transporter NanT"
                     /protein_id="HBA7630793.1"
                     /translation="MSTTTQNIPWYRHLNRAQWRAFSAAWLGYLLDGFDFVLIALVLT
                     EVQGEFGLTTVQAASLISAAFISRWFGGLMLGAMGDRYGRRLAMVTSIVLFSAGTLAC
                     GFAPGYITMFIARLVIGMGMAGEYGSSATYVIESWPKHLRNKASGFLISGFSVGAVVA
                     AQVYSLVVPVWGWRALFFIGILPIIFALWLRKNIPEAEDWKEKHGGKAPVRTMVDILY
                     RGEHRIANIVMTLAAATALWFCFAGNLQNAAIVAVLGLLCAAIFISFMVQSTGKRWPT
                     GVMLMVVVLFAFLYSWPIQALLPTYLKTDLAYDPHTVANVLFFSGFGAAVGCCVGGFL
                     GDWLGTRKAYVCSLLASQLLIIPVFAIGGANVWVLGLLLFFQQMLGQGISGILPKLIG
                     GYFDTDQRAAGLGFTYNVGALGGALAPILGALIAQRLDLGTALGSLSFSLTFVVILLI
                     GLDMPSRVQRWLRPEALRTHDAIDGKPFSGAVPFGSAKNDLVKTKS"
     gene            complement(9936..10829)
                     /gene="nanA"
                     /locus_tag="J1Y83_002655"
     CDS             complement(9936..10829)
                     /gene="nanA"
                     /locus_tag="J1Y83_002655"
                     /EC_number="4.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate lyase"
                     /protein_id="HBA7630794.1"
                     /translation="MATNLRGVMAALLTPFDQQQALDKASLRRLVQFNIQQGIDGLYV
                     GGSTGEAFVQSLSEREQVLEIVAEEAKGKIKLIAHVGCVSTAESQQLAASAKRYGFDA
                     VSAVTPFYYPFSFEEHCDHYRAIIDSADGLPMVVYNIPALSGVKLTLDQINTLVTLPG
                     VGALKQTSGDLYQMEQIRREHPDLVLYNGYDEIFASGLLAGADGGIGSTYNIMGWRYQ
                     GIVKALKEGDIQTAQKLQTECNKVIDLLIKTGVFRGLKTVLHYMDVVSVPLCRKPFGP
                     VDEKYLPELKALAQQLMQERG"
     gene            complement(10951..11742)
                     /gene="nanR"
                     /locus_tag="J1Y83_002656"
     CDS             complement(10951..11742)
                     /gene="nanR"
                     /locus_tag="J1Y83_002656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312126.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NanR"
                     /protein_id="HBA7630795.1"
                     /translation="MSPMNAFDPQAEDSTTTIGRNLRSRPLARKKLSEMVEEELEQMI
                     RRREFGEGEQLPSERELMAFFNVGRPSVREALAALKRKGLVQINNGERARVSRPSADT
                     IIGELSGMAKDFLSHPGGIAHFEQLRLFFESSLVRYAAEHATDEQIDLLAKALEINSQ
                     SLDNNAAFIRSDVDFHRVLAEIPGNPIFMAIHVALLDWLIAARPTVADQALHEHNNVS
                     YQQHIAIVDAIRRHDPDEADRALQSHLNSVSATWHAFGQTTNKKK"
     gene            complement(11850..12347)
                     /gene="sspB"
                     /locus_tag="J1Y83_002657"
     CDS             complement(11850..12347)
                     /gene="sspB"
                     /locus_tag="J1Y83_002657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClpXP protease specificity-enhancing factor"
                     /protein_id="HBA7630796.1"
                     /translation="MDLSQLTPRRPYLLRAFYEWLLDNQLTPHLVVDVTLPGVQVPME
                     YARDGQIVLNIAPRAVGNLELANDEVRFNARFGGIPRQVSVPLAAVLAIYARENGAGT
                     MFEPEAAYDEDASIMNDEEASADNETVMSVIDGDKPDHDDETHPDDEPPQPPRGGRPA
                     LRVVK"
     gene            complement(12353..12991)
                     /gene="sspA"
                     /locus_tag="J1Y83_002658"
     CDS             complement(12353..12991)
                     /gene="sspA"
                     /locus_tag="J1Y83_002658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stringent starvation protein A"
                     /protein_id="HBA7630797.1"
                     /translation="MAVAANKRSVMTLFSGPTDIYSHQVRIVLAEKGVSFEIEHVEKD
                     NPPQDLIDLNPNQSVPTLVDRELTLWESRIIMEYLDERFPHPPLMPVYPVARGESRLY
                     MHRIEKDWYTLMNTIINGSASEADAARKQLREELLAIAPVFGQKPYFLSDEFSLVDCY
                     LAPLLWRLPQLGIEFSGPGAKELKGYMTRVFERDSFLASLTEAEREMRLGRS"
     gene            complement(13386..13778)
                     /gene="rpsI"
                     /locus_tag="J1Y83_002659"
     CDS             complement(13386..13778)
                     /gene="rpsI"
                     /locus_tag="J1Y83_002659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000829824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HBA7630798.1"
                     /translation="MAENQYYGTGRRKSSAARVFIKPGNGKIVINQRSLEQYFGRETA
                     RMVVRQPLELVDMVEKLDLYITVKGGGISGQAGAIRHGITRALMEYDESLRSELRKAG
                     FVTRDARQVERKKVGLRKARRRPQFSKR"
     gene            complement(13794..14222)
                     /gene="rplM"
                     /locus_tag="J1Y83_002660"
     CDS             complement(13794..14222)
                     /gene="rplM"
                     /locus_tag="J1Y83_002660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HBA7630799.1"
                     /translation="MKTFTAKPETVKRDWYVVDATGKTLGRLATELARRLRGKHKAEY
                     TPHVDTGDYIIVLNADKVAVTGNKRTDKVYYHHTGHIGGIKQATFEEMIARRPERVIE
                     IAVKGMLPKGPLGRAMFRKLKVYAGNEHNHAAQQPQVLDI"
     gene            complement(14442..15569)
                     /locus_tag="J1Y83_002661"
     CDS             complement(14442..15569)
                     /locus_tag="J1Y83_002661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapE"
                     /protein_id="HBA7630800.1"
                     /translation="MQSVTPTSQYLKALNEGSHQPDDVQKEAVSRLEIIYQELINSTP
                     PAPRTSGLMARVGKLWGKREDTKHMPVRGLYMWGGVGRGKTWLMDLFYQSLPGERKQR
                     LHFHRFMLRVHDELTELQGQSDPLEIIADRFKAETDVLCFDEFFVSDITDAMLLGGLM
                     KALFARGITLVATSNIPPDELYRNGLQRARFLPAIDAIKRHCDVMNVDAGVDYRLRTL
                     TQAHLWLSPLNDETRAQMDKLWLALAGAKRENSPTLEINHRPLATMGVENQTLAVSFT
                     TLCVDARSQHDYIALSRLFHTVMLFDVPVMTRLMESEARRFIALVDEFYERHVKLVVS
                     AEVPLYEIYQGERLKFEFQRCLSRLQEMQSEEYLKREHLAG"
     gene            15763..16161
                     /locus_tag="J1Y83_002662"
     CDS             15763..16161
                     /locus_tag="J1Y83_002662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1043 family protein"
                     /protein_id="HBA7630801.1"
                     /translation="MTWEYALIGLVVGIIIGAVAMRFGNRKLRQQQALQYELEKNKAE
                     LDEYREELVSHFARSAELLDTMAHDYRQLYQHMAKSSSSLLPELSAEANPFRNRLAES
                     EASNDQAPVQMPRDYSEGASGLLRTGAKRD"
     gene            16315..17682
                     /gene="degQ"
                     /locus_tag="J1Y83_002663"
     CDS             16315..17682
                     /gene="degQ"
                     /locus_tag="J1Y83_002663"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine endoprotease DegQ"
                     /protein_id="HBA7630802.1"
                     /translation="MKKQTQLLSALALSVGLTLSASFQAVASIPGQVADQAPLPSLAP
                     MLEKVLPAVVSVRVEGTASQGQKIPEEFKKFFGDDLPDQPAQPFEGLGSGVIINANKG
                     YVLTNNHVINQAQKISIQLNDGREFDAKLIGSDDQSDIALLQIQNPSKLTQIAIADSD
                     KLRVGDFAVAVGNPFGLGQTATSGIISALGRSGLNLEGLENFIQTDASINRGNSGGAL
                     LNLNGELIGINTAILAPGGGSVGIGFAIPSNMARTLAQQLIDFGEIKRGLLGIKGTEM
                     SADIAKAFNLDVQRGAFVSEVLPGSGSAKAGVKAGDIITSLNGKPLNSFAELRSRIAT
                     TEPGTKVKLGLLRNGKPLEVEVTLDTSTSSSASAEMITPALEGATLSDGQLKDGGKGI
                     KIDEVVKGSPAAQAGLQKDDVIIGVNRDRVNSIAEMRKVLAAKPAIIALQIIRGNESL
                     YLLMR"
     gene            17772..18839
                     /gene="degS"
                     /locus_tag="J1Y83_002664"
     CDS             17772..18839
                     /gene="degS"
                     /locus_tag="J1Y83_002664"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane-stress sensor serine
                     endopeptidase DegS"
                     /protein_id="HBA7630803.1"
                     /translation="MFVKLLRSVAIGLIVGAILLVAMPSLRSLNPLSTPQFDSTDETP
                     ASYNLAVRRAAPAVVNVYNRGLNTNSHNQLEIRTLGSGVIMDQRGYIITNKHVINDAD
                     QIIVALQDGRVFEALLVGSDSLTDLAVLKINATGGLPTIPINPRRVPHIGDVVLAIGN
                     PYNLGQTITQGIISATGRIGLNPTGRQNFLQTDASINHGNSGGALVNSLGELMGINTL
                     SFDKSNDGETPEGIGFAIPFQLATKIMDKLIRDGRVIRGYIGIGGREIAPLHAQSGGM
                     DQLQGIVVNEVSPDGPAANAGIQVNDLIISVNNKPAISALETMDQVAEIRPGSVIPVV
                     VMRDDKQLTLQVTIQEYPATN"
     gene            complement(18902..19840)
                     /gene="mdh"
                     /locus_tag="J1Y83_002665"
     CDS             complement(18902..19840)
                     /gene="mdh"
                     /locus_tag="J1Y83_002665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000861594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HBA7630804.1"
                     /translation="MKVAVLGAAGGIGQALALLLKTQLPSGSELSLYDIAPVTPGVAV
                     DLSHIPTAVKIKGFSGEDATPALEGADVVLISAGVARKPGMDRSDLFNVNAGIVKNLV
                     QQVAKTCPKACIGIITNPVNTTVAIAAEVLKKAGVYDKNKLFGVTTLDIIRSNTFVAE
                     LKGKQPGEVEVPVIGGHSGVTILPLLSQVPGVSFTEQEVADLTKRIQNAGTEVVEAKA
                     GGGSATLSMGQAAARFGLSLVRALQGEQGVVECAYVEGDGQYARFFSQPLLLGKNGVE
                     ERKSIGTLSAFEQNALEGMLDTLKKDIALGEEFVNK"
     gene            20275..20745
                     /gene="argR"
                     /locus_tag="J1Y83_002666"
     CDS             20275..20745
                     /gene="argR"
                     /locus_tag="J1Y83_002666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001257852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ArgR"
                     /protein_id="HBA7630805.1"
                     /translation="MRSSAKQEELVKAFKALLKEEKFSSQGEIVAALQEQGFDNINQS
                     KVSRMLTKFGAVRTRNAKMEMVYCLPAELGVPTTSSPLKNLVLDIDYNDAVVVIHTSP
                     GAAQLIARLLDSLGKAEGILGTIAGDDTIFTTPANGFTVKELYEAILELFDQEL"
BASE COUNT         5033 a         5626 c         5459 g         4716 t
ORIGIN      
        1 agataattaa taaagaattt tgcgctaaag cacatttctg taccaataag cttgccattt
       61 gacctgtatc agctttcccg ataagttgga aatccgctgg aagctttctg gatgagcagc
      121 ctgctcatca tatttatgca gtaattgaga tcccctcttc accgtattaa ccgatgcgaa
      181 aaggacaaca agggggcgaa tgcgaggcgc gcgtatgaca cgcaaacccc gtcgccacgc
      241 tcttactgtg cccgtgcgca gcggttcgga agtggggttc ccgcagagcc tgggggaggt
      301 tcacgatatg ttgtacgata aatcccttga gagggataac tgtggtttcg gcctgatcgc
      361 ccacatagaa ggcgaaccta gccacaaggt agtgcgtact gcaatacacg cactggcccg
      421 catgcagcac cgtggcgcga ttctcgccga tggtaaaacc ggtgacggtt gcggcttgct
      481 gttacaaaaa ccggatcgtt tttttcgcat cgttgcgcag gagcgcggct ggcgtttagc
      541 aaaaaactac gccgtcggga tgctcttcct gaataaagat cctgaactcg ccgctgccgc
      601 acgccgcatc gttgaagaag agctgcaacg cgaaacgttg tcgattgtgg gctggcgtga
      661 tgtccccact aacgaaggcg tgctgggtga aatcgccctc tcctctctgc cacgcattga
      721 gcaaattttt gtgaacgccc cggcaggctg gcgtccgcgc gatatggagc gccgtctgtt
      781 tatcgcccgc cgccgcattg aaaagcgtct cgaagccgac aaagacttct acgtctgtag
      841 cctgtcgaat ctggtcaata tttataaagg tctgtgtatg ccggcggatc tgccgcgctt
      901 ctacctggac ctcgctgacc tgcgtctgga atcggccatc tgcctgtttc accagcgctt
      961 ttccactaac accgtaccgc gctggccgct ggcgcagccg ttccgctacc tcgcgcataa
     1021 cggtgaaatc aacaccatca ccggtaaccg ccaatgggcg cgtgcgcgta cctataaatt
     1081 ccagacgccg ctgatccctg acctgcacga cgccgcgccg ttcgtcaacg aaaccggctc
     1141 tgactccagt tcgatggata acatgctgga actcctgctg gcaggcggga tggatatcat
     1201 ccgtgccatg cgtctgttag tgccacctgc ctggcagaac aacccggata tggacccgga
     1261 actgcgtgcc ttctttgact ttaactccat gcatatggag ccgtgggatg gcccggcagg
     1321 catcgtgatg tctgacggtc gttttgccgc ctgtaacctc gaccgtaacg gtctgcgtcc
     1381 ggcgcgctac gtcatcacca aagataagct cattacctgc gcctctgaag tcggtatctg
     1441 ggattatcag cctgacgaag tggtcgaaaa aggccgcgtg gggccaggcg aactgatggt
     1501 tatcgacacc cgcagtgggc gtattctgca ctcggcagaa accgatgacg atctgaaaag
     1561 ccgccaccct tataaagagt ggatggagaa aaacgtccgc cgtctggtac cgtttgaaga
     1621 tctgcccgat gaagaagtgg gtagccgcga actggacgac gacacgcttg ccagctacca
     1681 gaaacaattt aactacagcg cggaagagct ggattcggtg atccgcgtac tgggtgaaaa
     1741 cggtcaggaa gcggtcggtt cgatgggcga cgacacccca ttcgccgtgc tctccagcca
     1801 gccgcgcatt atttacgact acttccgcca gcagtttgct caggtaacca acccgccaat
     1861 cgacccgctg cgcgaagcgc atgttatgtc gctcgccacc agtatcggtc gtgaaatgaa
     1921 cgtcttctgt gaagcagaag gccaggcgca ccgtttaagc tttaaatcgc cgattctgct
     1981 ctattccgat ttcaaacagc tcacgacgat gaaagaggat cactaccgcg cagatacgct
     2041 ggatatcacc tttgacgtca ctaaaaccac gctcgaagcg acggtgaaag agctgtgcga
     2101 caaagccgaa aaaatggtac gtagtggcac cgtgctgctg gtgctctccg accggaatat
     2161 cgctaaagat cgcctgccgg ttccggctcc aatggcggtt ggcgcgatcc agacccgtct
     2221 tgtcgagcaa agcctgcgtt gcgatgccaa catcatcgta gaaaccgcca gcgcccgtga
     2281 tccgcaccac ttcgccgtgt tgctgggttt cggcgcgacg gctatttatc cataccttgc
     2341 ctatgaaacg ctgggccgcc tggtagacac ccacgcgatt gccaaagatt atcgtaccgt
     2401 gatgctcaac tatcgtaacg gcatcaacaa aggcttgtac aaaatcatgt ccaaaatggg
     2461 catctccacc atcgcctctt atcgctgctc gaaactgttt gaagcggtcg gtctgcacga
     2521 tgatgtcgtg ggcctgtgct tccagggggc ggttagccgc attggggggg cgagctttga
     2581 agacttccag caggatctgc tgaatctgtc gaaacgtgcc tggctggcgc gcaagcccat
     2641 cagccagggc ggcctgctga aatacgtgca cggcggcgaa taccacgcct acaacccgga
     2701 cgtggtgcgc acactgcagc aggcggtaca aagcggcgag tatagcgact atcaggaata
     2761 cgcgaagctg gttaatgagc gtccggcaac cacgctacgc gatctgctgg caattacgcc
     2821 gggtgaaaac gcggtcaaca ttgctgatgt tgaaccggca agcgaactgt ttaaacgctt
     2881 cgataccgcc gcgatgtcta tcggcgcgtt aagcccggaa gcccacgagg cgctggcgga
     2941 agcgatgaac agcatcggcg gtaactcaaa ctccggcgaa ggcggtgaag acccggcgcg
     3001 ctatggcact aacaaagtgt cgcgcatcaa gcaggtggcg tccggtcgct tcggcgttac
     3061 tccggcgtat ctggtcaatg ccgacgtcat tcagattaaa gtcgcccagg gcgcgaagcc
     3121 gggagaaggc ggtcagttgc cgggtgataa agtcactcct tacattgcca aactgcgcta
     3181 ttcggtgccg ggcgtgacgc tgatctcccc gccgccgcac catgatatct actctatcga
     3241 ggacttagcg cagctcattt tcgacctcaa gcaggttaac ccgaaagcga tgatctccgt
     3301 gaagctggtg tccgaaccgg gcgtcggcac catcgcgacc ggcgtggcga aagcttatgc
     3361 ggacttgatc accatcgcag gctatgacgg cggcaccggc gcaagtccgc tttcatcggt
     3421 gaaatacgca ggctgtccgt gggaactcgg cctggtggaa acccaacagg cgctggttgc
     3481 caacggttta cgtcataaaa ttcgtttgca ggttgatggc ggcctgaaaa cgggcgttga
     3541 tatcatcaaa gcggcgattc tcggcgcaga aagcttcggc ttcggcactg gcccaatggt
     3601 cgcgctcggt tgtaaatatc tgcgaatttg ccacctgaac aactgcgcta cgggtgtagc
     3661 aactcaggat gacaaactgc gtaagaatca ctatcacggc ctgccgttca aagtgacgaa
     3721 ttactttgag tttatcgccc gtgaaacccg cgagctgatg gcacagctgg gcgtaacacg
     3781 tctggtggat ctgattggtc gcaccgacct gctgaaagag ctggacggtt tcaccgccaa
     3841 acagcagaaa ctggcgctgt cgaagctgct ggagactgcc gagccgcatc caggtaaggc
     3901 gctctactgc accgaaaaca acccgccgtt tgataacggc ctgctgaacg cgcagttgct
     3961 gcaacaggcg aaaccgtttg ttgatgaacg ccagagcaaa accttctggt tcgatatccg
     4021 caacaccgat cgttctgtcg gcgcatcgct ttcaggctat atcgcccaga cgcacggcga
     4081 tcaggggctg gctgccgatc ctatcaaagc gtacttcaac ggcaccgcag gccagagctt
     4141 cggcgtgtgg aacgcgggcg gcgtggaact gtacctgacc ggtgatgcca acgactatgt
     4201 cggtaaaggc atggcgggcg gcttaatcgc cattcgtcct ccggttggct cagccttccg
     4261 cagccatgaa gcgagcatta tcggcaacac ctgcctgtat ggcgcgaccg gtggtcgtct
     4321 gtatgccgca ggccgcgcgg gtgaacgttt cggcgtgcgt aactccggtg ctatcaccgt
     4381 ggtagaaggc attggcgaca acggctgtga atatatgacg ggtggtatcg tctgtattct
     4441 gggtaaaacc ggcgttaact tcggcgcggg catgactggc ggcttcgctt acgttctcga
     4501 tgaaagcggc gatttccgca aacgcgttaa cccggaactg gtcgaggtct taagcgttga
     4561 cgatctggcg atccatgaag agcatctgcg tggtcttatc accgagcatg ttcagcatac
     4621 cggttcccag cgtggtgaag agattctggc gaactggtca accttcgcca ctaaatttgc
     4681 gctggttaaa ccgaagtcca gtgatgtaaa agcactgctg ggtcaccgta gtcgtagcgc
     4741 agcagagttg cgcgtgcagg cgcagtaagg ggtagcaaca atgagtcaga atgtttatca
     4801 atttatcgac ctgcagcgcg ttgatccgcc aaagaaaccg ctgaagatcc gcaaaattga
     4861 gtttgttgaa atttacgagc cgttttccga aggccaggcc aaagcgcagg ctgaccgctg
     4921 cctgtcgtgc ggcaacccat actgcgagtg gaaatgcccg gtacacaact acatcccaaa
     4981 ctggctgaag ctcgccaatg agggacgtat ttttgaagcg gcggaactgt cgcaccagac
     5041 aaacaccctg ccggaagttt gcggacgtgt ctgcccgcaa gaccgtctgt gcgaaggttc
     5101 ctgcaccctg aacgatgagt tcggtgcggt gaccatcggc aacattgagc gttatatcaa
     5161 tgataaagcg ttcgagatgg gctggcgtcc ggatatgtcc ggcgtgaaac agaccggtaa
     5221 aaaagtggcg attatcggcg caggtccggc gggtctggcg tgcgcggatg tcctgacgcg
     5281 caacggcgta aaagcgatcg tcttcgaccg tcacccggaa attggcggct tgctgacctt
     5341 cggtattccg gctttcaagc tggaaaaaga ggtaatgacg cgccgccgtg aaatcttcac
     5401 cggcatgggt attgaattca aactcaatac cgaagtgggc cgcgacgtgc agctggacga
     5461 tctgctgagt gattacgatg ccgtgttcct tggcgtcggg acttatcagt caatgcgcgg
     5521 cgggctggaa aacgaagacg ccgatggcgt gtacgcagcg ctgccgttcc ttatcgccaa
     5581 caccaaacag ttaatgggct ttggtgaaac ccgcgacgaa ccgttcgtca gcatggaagg
     5641 caaacgcgtg atggtccttg gcggtggcga cactgcgatg gactgcgtgc gcacgtctgt
     5701 acgccagggg gcaaagcacg ttacctgtgc ctatcgtcgt gatgaagaga acatgccggg
     5761 ttcccgccgc gaagtgaaaa acgcgcggga agaaggcgta gagttcaaat tcaacgtcca
     5821 gccgctgggt atagaagtga atggcaacgg caaagtcagc ggcgtaaaaa tggtgcgcac
     5881 agaaatgggc gaaccggatg ccaaaggccg tcgccgcgcg gagattgtgg caggttccga
     5941 acatatcgtt ccggcagatg cggtgatcat ggcgtttggt ttccgtccac acaacatgga
     6001 atggctggca aaacacagcg tcgagctgga ttcgcagggg cgcattatcg ccccggaaga
     6061 caacgacaac gctttccaga ccagcaaccc gaaaatcttt gctggcggcg atatcgtccg
     6121 tggttccgat ctggtcgtta ccgccattgc cgaaggtcgc aaagcggcgg acggcattat
     6181 gaactggctg gaagtttaag cgaggtaaca ataaattcgc cagacagtgt ctggcgaatt
     6241 tataaaacga gcgatccaac taaattttaa gccattaaat cagccttaac cttcactacc
     6301 acctttttaa tctcaccagg ttcgccaaca acgcatcccg gtttatgcgg ttctcctggc
     6361 ataaacacgg cgaacattcc cggtttcagg atgatggctt gctcgttttc aatggcgctg
     6421 caaagctgat aatcatcttc atggtggaac tcttcacact gacgcgcagt gcctgccatg
     6481 ccaaacagaa tccgttcctc accgtttaat aacagctgga tatcaatgta ttgttcgtgc
     6541 aattcggctt ttttctcaac gggcgattga gtattaaatg tcatgacatt cataaagata
     6601 ttgtcgccct gtaattcgta acggccaggc gctttttctt gcggtctggc agctaatgcc
     6661 aacgttaacg cgtcctgtaa cgcaggatgt aatccggcag acggtaatga ctgtacttca
     6721 cccatcatca taatatttct ccctgggcca acagcgcagc cccaagtaaa cctgcatcgt
     6781 ggcggtagtg cgccgccagt aaatcaacat gaaatgccgc tggctcctgc gccagatacg
     6841 tttccaccag cgccagatac ccttctgcca gaccaacgct gccaccgacc accacgcact
     6901 ggcaatcagt tgtggcttta atatcagcga tcagccttgc aagcgtacgt gcggagcggt
     6961 gaatcagttg ctgcgcctgc tcatcgccct gcccggcgcg cgtgaaaata gttttcgcat
     7021 tcgcaccagc caactccccc tgcgctgccg ctgcaatgcc gcgaccagaa gcaatcgctt
     7081 ccacgcaacc tgtgcgtcca cagccgcaga ctggaccatg cggatcggca agcgtatgcc
     7141 cgatatgccc cgccagaccg ccagggccgg taagcagttt accgccgctc actacaccgc
     7201 cgccaacgcc ggtggaaaca gtgataaaca ccatatcggt tatatcgcca tccagcgcct
     7261 gatactccgc ccatgctgcg gcctgcgcat cattaatggc aatggtcggc aaattggtaa
     7321 gttgctccag cgttttgact aacggaaagt gtaacaatcc accgagatta tgcggattca
     7381 gcgccagcaa gctgccgtcg cgaataattc cggttgaagc gatagcaacc cgctgcgcat
     7441 gtgcttgcaa cggagagact aatgcggata aggcatcacg cagcgcctgc ggagtctggc
     7501 tggcaggcgt aggaagttct cggcgatcgc ggatctgccc gtcagcgcca atcagcgcgg
     7561 cggcaagttt agtaccaccg atatcaatcg ccagtgtggt catagcaccg cctttttcat
     7621 cgctgtgttg taccactgac aaatgtgttc aagacgcgta atggcagaac cgaccgtcac
     7681 cgcccacgcg ccgtggcgca tcgcatctgc cgcctgagca ggcgtgttgt aacgcccttc
     7741 ggcaatcacc cggcatccgg cgtcactcaa cgttttcacc agcgccagat ccggctcttc
     7801 tggcgtttca ggcgtggtat agccagaaag cgtagtgcca ataatttcgg ctcccagctt
     7861 ttggcatgcc aggccgtctt ccggcgttga gcagtcggtc atcgccagta aaccgtgatg
     7921 gtgaatacgt gccagcagcg tttcaacagg taccggacgc ggtcggtcgg tgccgtcaat
     7981 ggcgataatg tccgcgcccg cctgcgccag cgcatcaaca tcttcaatat aggccgtgat
     8041 gcgtaccgga gaatcctcca gatcgcgctt cacaatcccg ataatcggca cgctcaccac
     8101 cgcacgcgtg gcttgcagat ttgccacacc ttcaatacga atggcgaccg cgcccgcctg
     8161 ctctgccgct aatgccatgg cggcgacgat ttcgggttta tcgagcgggc tgtccggaac
     8221 cggctggcag gagacaatca ggccaccgtt agcagcgatt ttttgatcca gttgtgcaag
     8281 taacgacata catcttccct tagcgaaagg cccggtacag agaccgggca acaggattaa
     8341 cttttggttt tgactaaatc gtttttggcg ctgccaaacg gcacggcacc gctaaatggt
     8401 ttgccgtcga tagcgtcatg agtacgcaat gcttccgggc gcagccaacg ctgaacacga
     8461 gaaggcatat ccaaaccaat cagcaggatc accacaaacg ttaaactaaa cgagagcgag
     8521 ccaagtgccg ttcccagatc cagacgttga gcgatcaacg cgccgaggat tggagctagt
     8581 gcaccgccca gtgcgccaac gttgtaggta aagcccagcc ccgctgcacg ctggtcggta
     8641 tcgaaataac cgccgatcag tttcggtaaa atccctgaga tgccctgacc aagcatttgc
     8701 tggaagaaca gtaacagacc gagcacccag acgtttgcgc cgccaatcgc aaataccgga
     8761 ataatcagca gctgcgaggc cagcagacta caaacgtacg ctttgcgggt tcccagccag
     8821 tcaccgagga agccaccgac gcagcacccc accgccgcgc caaagccact aaagaacagc
     8881 acattggcga cagtatgcgg gtcataagcc agatcggttt tcagatacgt tggcagcagc
     8941 gcctgaatcg gccatgagta gaggaaagca aacaacacga ccaccatcag cattacgccc
     9001 gttggccagc gtttgcccgt actttgcacc ataaagctga taaagattgc ggcgcataac
     9061 agcccgagta cagcgacgat cgcggcattt tgcaggttac cggcgaagca gaaccacagc
     9121 gcagtagccg ccgccagtgt cattacgata ttggcaatgc gatgttcacc acggtagaga
     9181 atgtctacca tcgtgcgtac cggtgcttta cccccgtgtt tctctttcca gtcttccgct
     9241 tccgggatgt ttttacgcag ccagagagca aagatgattg gcaaaatgcc gataaagaac
     9301 agcgcacgcc agccccagac cggaaccacc aggctataga cctgagcggc aacgacggcc
     9361 cccactgaga agccggaaat cagaaaacca ctggctttgt tacgcagatg ctttggccag
     9421 ctttcaatga cataggtggc gctggaaccg tattcacccg ccatccccat gccgatgacc
     9481 agacgagcga taaacatggt gatgtagcct ggcgcaaagc cgcaggccag cgtcccggcc
     9541 gagaagagaa cgatgctggt gaccattgcc agacgacgcc cgtagcggtc acccatagcg
     9601 ccgagcatca ggccgccgaa ccagcgagag ataaaggctg cggagatcag acttgccgcc
     9661 tgcaccgtcg tgagcccgaa ttcaccctgt acttcggtga gtaccagggc gattaaaacg
     9721 aaatcaaaac cgtcaagcag atatcccaac caggcagcgg aaaatgcccg ccattgtgcg
     9781 cggttaagat ggcgatacca cgggatattc tgggttgtag tactcattgt gagtctcccg
     9841 cggtgggcga tgcccacacg ctttggtatg aaaattgtag ggtacagatg cgtttatttc
     9901 ccctcacccg gtaggggcga gcgaggggaa acaactcacc cgcgctcttg catcaactgc
     9961 tgggccagcg ccttcagttc tggcagatat ttttcatcta ccggaccaaa cggtttgcgg
    10021 cacagcggca cagaaacgac atccatataa tgcaggacag ttttcaggcc gcggaatacg
    10081 ccagttttga tcagtaaatc aatgactttg ttgcactcag tttgcagttt ctgcgcggtc
    10141 tggatatcgc cttctttcag cgccttaaca atgccctgat aacgccagcc catgatgttg
    10201 taagtgctgc cgataccgcc gtctgcgccc gccagaagac cagaggcgaa gatttcgtcg
    10261 taaccgttat agagcaccag atcaggatgt tcacgacgga tctgttccat ctgatagaga
    10321 tcgccagaag tctgtttcag cgcaccgacg ccaggcaacg taacaagtgt gttgatctga
    10381 tccagggtaa gttttacccc actcagggct ggaatgttgt acaccaccat cggcaaccca
    10441 tccgctgaat caataattgc ccgatagtga tcgcagtgtt cttcaaagct gaaaggatag
    10501 tagaacggcg tgacggcgga gacggcatcg aagccataac gtttagccga tgccgcaagt
    10561 tgttggcttt cggcggtgct gacgcaaccg acgtgggcga tgagtttaat cttacctttc
    10621 gcctcttcag cgacgatttc cagtacctgt tcacgctctg aaaggctctg tacaaaggct
    10681 tcgcctgtcg aaccacccac gtacaaaccg tcgatgccct gctgaatatt gaactgaacc
    10741 aggcggcgca gactcgcttt atccagtgct tgttgttggt caaaaggagt caggagtgca
    10801 gccattacgc cacgtaaatt cgttgccata aatacctctg aagtgatgct tgtctgataa
    10861 acgatatacc tttatacctg ttataccaga tcaattaagc aacaccccat acagaaagct
    10921 tataatgcga tctgcttcac taaagtggca ttatttcttt ttgttggtgg tctgaccgaa
    10981 ggcgtgccag gtagcagaga cgctgttgag atgcgattgc aacgcgcgat cggcttcgtc
    11041 aggatcatga cggcggatcg cgtcaacgat cgcaatatgc tgttgatagc taacgttgtt
    11101 atgctcatgc agagcctgat cggccacggt tggacgtgcg gcgattagcc agtcgagcag
    11161 ggcaacgtgg atcgccatga agattgggtt accagggatc tccgccagaa cgcggtggaa
    11221 atcaacgtct gagcgaatga atgccgcgtt gttatccagc gactgactgt tgatttccag
    11281 tgcttttgcc agcaaatcga tttgctcatc ggtggcgtgt tcagccgcat agcgcaccag
    11341 gctggattca aagaacagac gtaattgttc gaaatgggca atcccaccag gatgagaaag
    11401 gaaatctttc gccatgccgg aaagctcgcc gatgatggtg tccgcagaag gacgtgagac
    11461 gcgggcacgt tcgccgttgt ttatttgcac cagacctttg cgttttaacg ctgccagcgc
    11521 ttcgcgcacc gaaggacgcc cgacgttaaa gaacgccatc agttcgcgtt cagacggtaa
    11581 ttgttcacct tcgccaaatt cacgacggcg gatcatctgt tccagctctt cttccaccat
    11641 ttcggagagt tttttacgcg ccagcgggcg gctgcgcaag ttgcgcccga tagtggtagt
    11701 agaatcttca gcttgcggat caaatgcgtt cataggactc attcaggaaa atgcgttagc
    11761 ggataataac gacgctatcg tagcacagta tcaaagtcag gcgaaaagat gacagatagc
    11821 aaaacaggcc acatgggcct gttttcattt tacttcacaa cgcgtaatgc cggtcgacca
    11881 ccgcgtggtg gctgcggagg ttcatcgtca ggatgagttt catcatcgtg atctggcttg
    11941 tcgccatcga taaccgacat aacggtttcg ttgtctgccg atgcctcttc atcattcatg
    12001 atgctggcat cttcatcgta ggcagcttca ggctcaaaca tcgtgcctgc gccattttca
    12061 cgggcgtaga tagctagcac agcagccagc ggcacagaaa cctgacgcgg aatgccacca
    12121 aagcgcgcgt taaagcgcac ctcatcattc gccagttcca gattgccgac agcacgcggc
    12181 gcaatgttga gtacgatttg cccgtcacgc gcatattcca taggaacctg cacgccaggg
    12241 agcgtcacat ccaccaccag gtgcggcgtg agctggttat ccagcaacca ctcatagaat
    12301 gcacgcagca gatagggacg acgtggtgtt agctgtgaca aatccataca gattaactcc
    12361 ggcccagacg catttcacgt tctgcttcag ttaaagaagc aaggaaagag tcacgttcaa
    12421 agacgcgggt catatagcct ttcagctctt tcgcacccgg gccgctgaac tcgatgccca
    12481 gttgcggcag acgccacagc agaggagcaa ggtagcaatc gaccaggctg aactcatcgc
    12541 tcaggaagta cggcttctga ccgaagaccg gcgcaatcgc cagcagttct tcgcgcagtt
    12601 gcttacgtgc ggcatcggct tcagaagctg aaccgttgat gatggtgttc atcagcgtgt
    12661 accagtcttt ttcgatgcga tgcatgtaca gacggctttc accgcgagct accgggtaaa
    12721 caggcatcag tggcggatgc gggaaacgct catccagata ttccataatg atgcgagatt
    12781 cccacagggt cagctcacga tccaccaggg tcggaacgct ctgattcggg ttgaggtcaa
    12841 tcagatcctg aggcggattg tccttttcca cgtgttcgat ctcgaaactt acacctttct
    12901 cagccagcac aatgcggacc tgatggctat agatgtcagt aggaccggaa aacagcgtca
    12961 ttaccgaacg tttgttggca gcgacagcca tgaaaacctc caggtatagt cagaattttt
    13021 actgctacca gccaccaggt ggccagtcag aagttgtgtt acccagtaag gaacgactct
    13081 ctttgttcga aaatcaaaca aaaaatgagc aatacccgac atttgggcag aaaattggat
    13141 gatagtttac cagattttgc gacctttgtg gtgagtcgat gccggaaatg gggaaaaaga
    13201 gatgcgcttt agtctgaaat agttgactta gtcccttatt ggcgatgtgg tttttgtttt
    13261 acctgtctgt caggtggcag caaaaagcaa ctttccattt tttacgctga tgcagatttt
    13321 agccataaaa aaacccgccg aagcgggttt tttcgcaaat tgttttctgc cggagcagaa
    13381 gccaattaac gtttggagaa ctgcggacga cgacgtgctt tacgcagacc gactttctta
    13441 cgttcaacct gacgagcgtc acgagtaacg aagccagctt tacgcagttc agaacgcagg
    13501 gactcgtcgt attccatcag agcgcgggtg ataccgtgac ggatcgcacc agcctgacca
    13561 gagataccac cacctttaac ggtgatgtac aggtccagtt tctcaaccat gtcgaccagt
    13621 tccagcggct gacgaactac catgcgagca gtttcacgac cgaagtactg ttccagagaa
    13681 cgttggttga ttacgatttt accgttgccc ggtttgatga aaacgcgagc tgcggaactt
    13741 ttgcggcgac cagtgccgta gtattgattt tcagccattg cctataatcc cgattagatg
    13801 tcaagaactt gcggttgctg tgccgcgtgg ttgtgctcgt tacccgcgta aactttcagt
    13861 ttacggaaca tagcacgacc cagcgggcct tttggcaaca tgcctttaac cgcgatttca
    13921 atcacacgct caggacggcg agcaatcatc tcttcaaagg tcgcttgttt gataccaccg
    13981 atgtggccgg tgtggtgata gtacactttg tcagtacgct tgttgccggt tacagcaact
    14041 ttgtcagcgt tcagaacgat gatgtaatca ccggtatcta cgtgcggagt gtattccgct
    14101 ttgtgcttac cgcgcaggcg acgagccagt tcagtagcca gacggcccag agttttaccg
    14161 gtcgcgtcaa caacatacca gtcgcgtttt acggtttctg gtttagctgt aaaagttttc
    14221 attaaaagct tacccaataa atagttacac gttggtgaac acccaaacgt cttcaattgt
    14281 tgaggttcac acgacaaagt ccggcaaacc taccccttcg aatagcctat gccagcacac
    14341 aaaaagtttc gggaaaaaaa actttcttgt aacgtggggt cgcaggatta tagagaagtc
    14401 ggggtcaaag atcgacccct ttttgtgatt tgtggcaggt tttaccccgc caaatgctcg
    14461 cgcttcaaat actcttcgct ttgcatctct tgcagacgtg acaggcaacg ctggaactca
    14521 aacttcagcc gctcgccctg ataaatttca tacagcggca cttctgcact caccactaat
    14581 ttgacatggc gctcgtaaaa ctcatccacc agcgcaataa agcgccgcgc ttcgctctcc
    14641 atcaaccgcg tcataactgg tacatcaaac aacatgaccg tgtgaaagag gcgtgagagc
    14701 gcaatatagt catgctgact gcgggcgtcg acgcacagcg tagtaaaaga aaccgccagc
    14761 gtctggttct cgacgcccat tgtcgccaat ggccgatggt tgatttctaa cgtcggtgaa
    14821 ttttctcgtt tcgcccccgc cagcgccaac catagtttat ccatttgcgc ccgggtttca
    14881 tcgttgaggg gcgaaagcca cagatgcgcc tgagtgagtg tacgcagtcg ataatcaaca
    14941 ccagcgtcca cgttcattac atcacaatgc cgtttaatag catcgattgc aggcaaaaaa
    15001 cgcgcacgtt gcaggccatt tcgataaagt tcgtccggcg gaatatttga cgtcgctacc
    15061 agcgttattc cgcgagcgaa cagggctttc atcaggccgc caagtagcat ggcgtcggta
    15121 atatcagaaa caaaaaattc gtcaaaacaa agcacgtcag tttctgcttt aaagcgatcg
    15181 gcaataattt ccagcggatc gctttggcct tgtaattcgg tcagctcatc gtgcacccgc
    15241 agcataaaac gatgaaagtg caggcgctgt ttccgctctc ccggcagact ttgatagaaa
    15301 aggtccatca gccaggtttt cccgcgtcct acaccgcccc acatatataa gccacgcact
    15361 ggcatatgct ttgtgtcttc gcgtttaccc cacagcttac cgacccgcgc cattagccca
    15421 ctcgtcctgg gggctggtgg cgtgctattg atgagttcct gataaataat ttccaggcgg
    15481 ctgacggcct ctttttgaac gtcgtcgggt tgatggctgc cttcattgag cgccttcagg
    15541 tattgcgatg ttggggtaac gctttgcatg atcttattgt tattccttga ataatcggtg
    15601 cgccgttgtt cacggttgac gaaaaaaagg ccgttctaca ctacgcgata tgctgtcggg
    15661 attccacttc tgtggaatta acggttatag tggcataatc agccgcaggc atggagcctg
    15721 aagccaacac cctacggaaa caaaagacaa cgggagatgt tcatgacctg ggaatatgcg
    15781 ctaattgggt tagtcgtcgg tatcattatt ggtgctgtgg ccatgcgttt tggtaatcgt
    15841 aaactacgcc agcaacaggc gttgcagtac gaactggaaa agaataaagc tgaactggac
    15901 gagtatcgcg aagagctggt tagccacttt gcccgcagcg cggaattact ggataccatg
    15961 gcgcacgact atcgccagct gtatcagcac atggcgaaaa gctccagtag cctgctgccg
    16021 gaactgtctg cggaagcaaa cccgttccgt aatcgtctgg cagagtctga agccagcaac
    16081 gatcaggcac cggtgcagat gcctcgcgac tattctgaag gcgcatccgg cctgctgcgt
    16141 actggcgcga agcgcgacta atttattttt cgggcgcagc cattgcgccc tcctcttctc
    16201 tccctccccg actatcattt aatctggtgt ctcattgtta gccgtctgaa aattcaataa
    16261 catcaaactg ttttgaatct cttttcttat cattcaggta cgagagcagg aataatgaaa
    16321 aaacaaaccc agctgttgag tgcattagcg ttaagtgtcg ggttaactct ctcggcgtca
    16381 tttcaggccg tcgcgtcgat tccaggccag gttgccgatc aggcccccct ccccagtctg
    16441 gctccaatgc tggaaaaagt gcttccggca gtggtgagcg tacgggtgga aggaacggcc
    16501 agtcagggac agaaaatccc ggaagaattc aaaaagtttt ttggtgatga tttaccggat
    16561 caacctgcac aacccttcga aggtttaggc tccggtgtca tcatcaacgc caataaaggc
    16621 tatgtgctga ccaacaacca tgtgattaat caggcacaga aaatcagtat tcagctcaat
    16681 gatgggcgcg agtttgatgc aaaactgatt ggtagcgatg accagagcga tatcgccctg
    16741 ttgcaaattc aaaacccgag caaattaacg caaatcgcta ttgccgactc cgataaattg
    16801 cgcgtcggtg attttgccgt ggcggtcggt aacccatttg gccttgggca aaccgctacc
    16861 tccgggatta tatccgcatt aggccgcagc ggtctgaatc tcgaaggtct ggaaaacttt
    16921 attcaaactg atgcctccat taaccgcggc aattccggtg gtgcgctgtt aaaccttaac
    16981 ggtgagttaa ttggcatcaa cactgcaatc cttgcgcctg gcggcggtag cgtcgggatt
    17041 ggatttgcca ttcccagtaa tatggcgcga acactggcgc agcagcttat cgactttggt
    17101 gaaatcaaac gcggtctgtt aggcatcaaa ggcaccgaga tgagtgccga tatcgccaaa
    17161 gccttcaacc ttgacgtgca gcgtggcgcg tttgtcagcg aagtgttgcc aggttctggc
    17221 tcggcgaaag cgggcgtcaa agcgggcgat attattacca gcctcaacgg caaaccgctg
    17281 aatagctttg ctgagttgcg ctctcgtatc gcgaccaccg agccgggcac aaaagtgaag
    17341 cttggcctgc tgcgtaacgg caaaccactg gaagtagaag tgacgctcga taccagcacc
    17401 tcttcgtcgg ccagcgctga aatgatcacg ccagcgctgg aaggtgcaac gttgagcgat
    17461 ggtcagctaa aagatggtgg caaaggtatt aagatcgatg aggttgtcaa aggaagccca
    17521 gctgctcagg ctggcttgca aaaagacgat gtgatcattg gcgtcaaccg cgatcgggtg
    17581 aactcgattg ctgaaatgcg taaagtgctg gcggcaaaac cggccataat cgccttgcaa
    17641 atcatacgcg gcaatgaaag cctctaccta ctaatgcgtt aatgtcgtaa accgggcatc
    17701 aggctgacgt gtgatgtccg gttaactcgt ggtatgctgc tgccgttccc ttttttaacg
    17761 atgcctccat catgtttgtg aagctcttac gttccgttgc gattggatta attgtcggcg
    17821 ctattctgct ggttgccatg ccttcgctgc gcagccttaa cccgctttcc actccgcaat
    17881 ttgacagtac cgatgagacg cctgccagct ataatctggc ggttcgccgc gccgcgccag
    17941 cggtggttaa cgtttataac cgcggtttga acaccaactc tcacaaccag cttgagatcc
    18001 gcaccctggg atctggtgta atcatggatc aacgcgggta tatcatcacc aataaacacg
    18061 tgatcaacga cgccgatcag atcatcgtcg ccttacagga tggacgtgtt tttgaagcgt
    18121 tactggttgg ctctgactct ctaaccgatc tggcggtgct gaaaattaac gccacaggcg
    18181 gtttaccaac gattccgatc aatccacgcc gcgtaccgca cattggcgat gtggttctgg
    18241 cgatcggtaa cccgtacaac ctcgggcaga ccattaccca ggggattatc agtgccacgg
    18301 gtcgaatcgg tctgaacccg accgggcggc aaaacttcct gcaaaccgat gcttccatta
    18361 accacggtaa ctctggcggc gcgctggtga actcgctggg cgaactgatg ggcattaaca
    18421 cgctgtcgtt tgataagagt aacgatggcg aaacgccgga aggtatcggc tttgcgattc
    18481 ctttccagtt agcgaccaaa attatggata aactgatccg tgatggccgt gtgatccgtg
    18541 gctacattgg cattggtgga cgtgagatcg ccccattaca tgcgcagagt ggcggcatgg
    18601 atcaactgca agggatcgtg gttaacgaag tgtcgcctga tggcccagcg gcaaatgcag
    18661 gcatccaggt caacgatctg attatctctg tgaataacaa acccgctatt tccgccctgg
    18721 agacaatgga tcaggtcgcg gaaattcgcc ctggttcggt gatcccggtt gtcgtgatgc
    18781 gtgacgataa acagttaaca ttgcaggtca ccattcagga atatccggcg accaactaag
    18841 gtcagcgtaa aaaaacaaaa accggagtct gtgctccggt tttttattat ccgctaatca
    18901 attacttatt aacgaactct tcgcccaggg cgatatcttt cttcagcgta tccagcatac
    18961 cttccagcgc gttctgttca aatgcgctca gagtaccgat agatttacgc tcttccacgc
    19021 cgtttttacc cagcagcagc ggttgagaga agaaacgagc gtactgaccg tcgccttcaa
    19081 cgtaggcaca ttcgacaacg ccttgttcgc cctgcagtgc gcgaaccaga gacagaccaa
    19141 aacgtgcagc tgcctggccc atagacaggg ttgcagaccc gccaccggct ttcgcttcaa
    19201 ccacctcagt acccgcgttc tggatacgtt tggtcagatc agccacttcc tgctcggtaa
    19261 aactaacgcc aggaacctgt gacagcagcg gcagaatggt aacaccagag tgaccaccaa
    19321 taaccggcac ttcaacttcg cctggctgtt tgcctttcag ttccgcaaca aaggtgttgg
    19381 aacgaatgat atccagcgtg gtaacgccga acagtttgtt tttgtcataa acaccggctt
    19441 ttttcagcac ttcagcagca atcgcaactg tagtgttaac cgggttagtg ataataccaa
    19501 tgcacgcttt cgggcaggtt ttcgcaactt gctgtaccag gtttttcacg atgccggcgt
    19561 taacgttaaa caggtcggaa cgatccatac ccggtttacg cgctacgcct gcagagataa
    19621 gaacgacatc tgcaccttcc agcgccggag tcgcatcttc acctgaaaaa cctttgattt
    19681 tcacagcagt agggatatgg ctcagatcga cagccacacc gggagtcact ggagcgatat
    19741 catacagaga gagttctgaa cctgaaggca gttgggtttt taacagtagt gcaagcgcct
    19801 ggccaatacc gccagcagcg ccgaggactg cgactttcat cctaaactcc ttattatatt
    19861 gataaactaa gatatgttgc tccgctgccg cgaccttaat ccacaaaatt gccgtttaca
    19921 atgaccacat ctcaagaatg tgtagtcacg caagtttagc gtttatgcat ttaattgccg
    19981 taatcaggaa cctaacttac gtaattaaca gtcacttatg cattagcgca acattccaac
    20041 aggtggtgac aatataccct accgctcagc caaaacaata tcaatttgat aacaattaat
    20101 ttacttttaa gcagaatttg caggccgtga cgcaggcatg tttctcaata acgaaatttg
    20161 ataaaatccc gctctttcat aacattattt cagccttctt cagggctgac tgtttgcata
    20221 aaaattcatc tgtatgcaca ataatgttgt atcaaccacc atatcgggtg acttatgcga
    20281 agctcggcta agcaagaaga actagttaaa gcatttaaag cattacttaa agaagagaaa
    20341 tttagctccc agggcgaaat cgtcgccgcg ttgcaggagc aaggctttga caatattaat
    20401 cagtctaaag tctcgcggat gttgaccaag tttggtgctg tacgtacacg caatgccaaa
    20461 atggaaatgg tttactgcct gccagctgaa ctgggtgtac caaccacctc cagtccattg
    20521 aagaatctgg tactggatat cgactacaac gatgcagttg tcgtgattca taccagccct
    20581 ggtgcggcgc agttaattgc tcgcctgctg gactcactgg gcaaagcaga aggtattctg
    20641 ggcaccatcg ctggcgatga caccatcttt actacccctg ctaacggttt caccgtcaaa
    20701 gagctgtacg aagcgatttt agagctgttc gaccaggagc tttaatctct gccccgtcgc
    20761 ttctggcggc ggggaaaatg ttgcttttgc ctatcaaccc cctgctttcc cctgcgattc
    20821 atttaacgaa tagt
//
LOCUS       DADSYM010000085        20322 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2415
            SAMN08039579-rid11723173.denovo.085, whole genome shotgun sequence.
ACCESSION   DADSYM010000085 DADSYM010000000
VERSION     DADSYM010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039579
            Sequence Read Archive: SRR6362533
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 20322)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20322)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980162.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 03:39:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,484
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 4,347
            CDSs (with protein)               :: 4,347
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 43 of 85
            Pseudo Genes (incomplete)         :: 37 of 85
            Pseudo Genes (internal stop)      :: 19 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20322
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039579-rid11723173.denovo.085"
                     /strain="LGE2415"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8302 N 95.9628 W"
                     /collection_date="2015-08-24"
     gene            complement(188..1561)
                     /gene="argH"
                     /locus_tag="J1Y83_002667"
     CDS             complement(188..1561)
                     /gene="argH"
                     /locus_tag="J1Y83_002667"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HBA7630806.1"
                     /translation="MALWGGRFTQAADQRFKQFNDSLRFDYRLAEQDIVGSVAWSKAL
                     VTVGVLTAEEQAQLEEALNVLLEDVRARPQQILESDAEDIHSWVEGKLIDKVGQLGKK
                     LHTGRSRNDQVATDLKLWCKDTVSELLTANRQLQSALVETAQNNQDAVMPGYTHLQRA
                     QPVTFAHWCLAYVEMLARDESRLQDALKRLDVSPLGCGALAGTAYEIDREQLAGWLGF
                     ASATRNSLDSVSDRDHVLELLSAAAIGMVHLSRFAEDLIFFNTGEAGFVELSDRVTSG
                     SSLMPQKKNPDALELIRGKCGRVQGALTGMMMTLKGLPLAYNKDMQEDKEGLFDALDT
                     WLDCLHMAALVLDGIQVKRPRCQEAAQQGYANATELADYLVAKGVPFREAHHIVGEAV
                     VEAIRQGKALEDLPLSELQKFSQVIGEDVYPILSLQSCLDKRAAKGGVSPQQVAQAIA
                     FAQARLG"
     gene            complement(1622..2398)
                     /gene="argB"
                     /locus_tag="J1Y83_002668"
     CDS             complement(1622..2398)
                     /gene="argB"
                     /locus_tag="J1Y83_002668"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418394.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HBA7630807.1"
                     /translation="MMNPLIIKLGGVLLDSEEALERLFSALVNYRESHQRQLVIVHGG
                     GCVVDELMKGLNLPVKKKNGLRVTPADQIDIITGALAGTANKTLLAWAKKHQIAAVGL
                     FLGDGDSVKVTQLDEELGHVGLAQPGSPKLINSLLENGYLPVVSSIGVTNEGQLMNVN
                     ADQAATALAATLGADLILLSDVSGILDGKGQRIAEMTAAKAEQLIEQGIITDGMIVKV
                     NAALDAARTLGRPVDIASWRHAEQLPALFNGMPMGTRILA"
     gene            complement(2406..3410)
                     /gene="argC"
                     /locus_tag="J1Y83_002669"
     CDS             complement(2406..3410)
                     /gene="argC"
                     /locus_tag="J1Y83_002669"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HBA7630808.1"
                     /translation="MLNTLIVGASGYAGAELVTYVNRHPHMNITALTVSAQSNDAGKL
                     ISDLHPQLKGIVDLPLQPMSDISEFSPGVDVVFLATAHEVSHDLAPQFLEAGCVVFDL
                     SGAFRVNDVAFYEKYYGFTHQYPELLEQAAYGLAEWCGNKLKEANLIAVPGCYPTAAQ
                     LALKPLIDADLLDLNQWPVINATSGVSGAGRKAAISNSFCEVSLQPYGVFTHRHQPEI
                     ATHLGADVIFTPHLGNFPRGILETITCRLKPGVSQVQVAQALQQAYAHKPLVRLYDKG
                     VPALKNVVGLPFCDIGFAVQGEHLIIVATEDNLLKGAAAQAVQCANIRFGYAETQSLI
                     "
     gene            3564..4715
                     /gene="argE"
                     /locus_tag="J1Y83_002670"
     CDS             3564..4715
                     /gene="argE"
                     /locus_tag="J1Y83_002670"
                     /EC_number="3.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylornithine deacetylase"
                     /protein_id="HBA7630809.1"
                     /translation="MKNKLPPFIEIYRALIATPSISATEEALDQSNADLITLLADWFK
                     DLGFNVEVQPVPGTRNKFNMLASCGQGAGGLLLAGHTDTVPFDDGRWTRDPFTLTEHD
                     GKLYGLGTADMKGFFAFILDALRDVDVTKLAKPLYILATADEETSMAGARYFAETTAL
                     RPDCAIIGEPTSLQPVRAHKGHISNAIRIQGQSGHSSDPARGVNAIELMHDTIGHILQ
                     LRDNLKERYHYDAFTVPYPTLNLGHIHGGDASNRICACCELHMDIRPLPGMTLNELNG
                     LLNDALAPVSERWPGRLTVDELHPPIPGYECPPNHKLVEVVEKLLGAKTEVVNYCTEA
                     PFIQTLCPTLVLGPGSINQAHQPDEYLETRFIKPTRELITQVIHHFCWH"
     gene            5017..7668
                     /gene="ppc"
                     /locus_tag="J1Y83_002671"
     CDS             5017..7668
                     /gene="ppc"
                     /locus_tag="J1Y83_002671"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HBA7630810.1"
                     /translation="MNEQYSALRSNVSMLGKVLGETIKDALGEHILERVETIRKLSKS
                     SRAGNDANRQELLTTLQNLSNDELLPVARAFSQFLNLANTAEQYHSISPKGEAASNPE
                     VIARTLRKLKNQPQLSEDTIKKAVESLSLELVLTAHPTEITRRTLIHKMVEVNACLKQ
                     LDNKDIADYEHNQLMRRLRQLIAQSWHTDEIRKLRPSPVDEAKWGFAVVENSLWQGVP
                     NYLRELNEQLEENLGYKLPVEFVPVRFTSWMGGDRDGNPNVTADITRHVLLLSRWKAT
                     DLFLKDIQVLVSELSMVEATPELLALVGEEGAAEPYRYLMKNLRSRLMATQAWLEARL
                     KGEELPKPEGLLTQNEELWEPLYACYQSLQACGMGIIANGDLLDTLRRVKCFGVPLVR
                     IDIRQESTRHTEALGELTRYLGIGDYESWSEADKQAFLIRELNSKRPLLPRNWQPSAE
                     TREVLDTCQVIAEAPQGSIAAYVISMAKTPSDVLAVHLLLKEAGIGFAMPVAPLFETL
                     DDLNNANDVMTQLLNIDWYRGLIQGKQMVMIGYSDSAKDAGVMAASWAQYQAQDALIK
                     TCEKAGIELTLFHGRGGSIGRGGAPAHAALLSQPPGSLKGGLRVTEQGEMIRFKYGLP
                     EITVSSLSLYTGAILEANLLPPPEPKESWRRIMDELSVISCDLYRGYVRENKDFVPYF
                     RSATPEQELGKLPLGSRPAKRRPTGGVESLRAIPWIFAWTQNRLMLPAWLGAGTALQK
                     VVEDGKQSELEAMCRDWPFFSTRLGMLEMVFAKADLWLAEYYDQRLVDKALWPLGKEL
                     RNLQEEDIKVVLAIANDSHLMADLPWIAESIQLRNIYTDPLNVLQAELLHRSRQAEKE
                     GQEPDPRVEQALMVTIAGIAAGMRNTG"
     gene            7851..9584
                     /gene="cptA"
                     /locus_tag="J1Y83_002672"
     CDS             7851..9584
                     /gene="cptA"
                     /locus_tag="J1Y83_002672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase CptA"
                     /protein_id="HBA7630811.1"
                     /translation="MHSTEVQAKPLFSWKALGWALLYFWFFSTLLQAIIYISGYSGTN
                     GIRDSLLFSSLWLIPVFLFPKRIKIIAAVIGVVLWAASLAALCYYVIYGQEFSQSVLF
                     VMFETNTNEASEYLSQYFSLKIVLIALAYTAVAVLLWTRLRPVYIPKPWRYVVSFALL
                     YGLILHPIAMNTFIKNKPFEKTLDNLASRMEPAAPWQFLTGYYQYRQQLNSLTKLLNE
                     NNALPPLANFKDESGNEPRTLVLVIGESTQRGRMSLYGYPRETTPELDALHKTDPNLT
                     VFNNVVTSRPYTIEILQQALTFANEKNPDLYLTQPSLMNMMKQAGYKTFWITNQQTMT
                     ARNTMLTVFSRQTDKQYYMNQQRTQSAREYDTNVLKPFQEVLKDPAPKKLIIVHLLGT
                     HIKYKYRYPEDQGKFDGNTEHVPPGLNAEELESYNDYDNANLYNDHVVASLIKDFKAT
                     DPNGFLVYFSDHGEEVYDTPPHKTQGRNEDNPTRHMYTIPFLLWTSEKWQATHPRDFS
                     QDVDRKYSLAELIHTWSDLAGLSYDGYDPTRSVVNPQFKETTRWIGNPYKKNALIDYD
                     TLPYGDQVGNQ"
     gene            9810..10661
                     /locus_tag="J1Y83_002673"
     CDS             9810..10661
                     /locus_tag="J1Y83_002673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBA7630812.1"
                     /translation="MYHDVSYLLSRLINGPLSLRQIYFASSNGPVPDLAYQVDFPRLE
                     IVLEGEFIDTGARAALVPGDVLYVPAGGWNFPQWQAPATTFSVLFGKQQLGFSVVQWD
                     GKQYQNLAKQHVARRGPRIGSFLLQTLNEMQMQSQEQQTARLIVTSLLSHCRDLLGSQ
                     IQTASRSQALFEAIRDYIDERYASALTRESVAQAFYISPNYLSHLFQKTGAIGFNEYL
                     NHTRLEHAKTLLKGYDLKVKEVAHACGFVDSNYFCRLFRKNTERSPSEYRRQYHSQLT
                     EKPTTPE"
     gene            complement(10648..10989)
                     /locus_tag="J1Y83_002674"
     CDS             complement(10648..10989)
                     /locus_tag="J1Y83_002674"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIB"
                     /protein_id="HBA7630813.1"
                     /translation="MAYLVAVTACVSGVAHTYMAAERLEKLCQLEKWGVSIETQGALG
                     TENRLADEDIRRADVALLITDIELAGAERFEHCRYVQCSIYAFLREPQRVMSAVRKVL
                     SAPQQTHLILE"
     gene            complement(10991..11869)
                     /locus_tag="J1Y83_002675"
     CDS             complement(10991..11869)
                     /locus_tag="J1Y83_002675"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418387.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[formate-C-acetyltransferase]-activating enzyme"
                     /protein_id="HBA7630814.1"
                     /translation="MTSSAGQRISCNVVETRRDDVARIFNIQRYSLNDGEGIRTVVFF
                     KGCPHLCPWCANPESISGKIQTVRREAKCLHCAKCLRDADECPSGAFERIGRDISLDA
                     LEREVMKDDIFFRTSGGGVTLSGGEVLMQAEFATRFLQRLRLWGVSCAIETAGDAPAS
                     KLLPLAKLCDEVLFDLKIMDAAQARDVVKMNLPRVLENLRLLVSEGVNVIPRLPLIPG
                     FTLSRENMQQALDVLIPLNIKQIHLLPFHQYGEPKYRLLGKTWSMKEVAAPSSADVAT
                     MREMAERAGFQVTVGG"
     gene            complement(11835..14132)
                     /locus_tag="J1Y83_002676"
     CDS             complement(11835..14132)
                     /locus_tag="J1Y83_002676"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HBA7630815.1"
                     /translation="MTNRISRLKTALFANTREISLERALLYTASHRQTEGEPVIMRRA
                     KATAYILEHVEISIRDEELIAGNRTVKPRAGIMSPEMDPYWLLKELDQFPTRPQDRFA
                     ISEEDKRIYREELFPYWEKRSMKDFINGQMTDEVKAATSTQIFSINQTDKGQGHIIID
                     YPRLLNHGLGELVAQMQQHCQQQPENHFYQAALLLLEASQKHILRYAELAETMAASCT
                     DGQRREELLTIAEISRHNAEHKPQTFWQACQLFWYMNIILQYESNASSLSLGRFDQYM
                     LPFYQASLTQGEDPAFLKELLESLWVKCNDIVLLRSTSSARYFAGFPTGYTALLGGLT
                     ENGRSAVNVLSFLCLDAYQSVQLPQPNLGVRTNALIDTPFLMKTAETIRLGTGIPQIF
                     NDEVVVPAFLNRGVSLEDARDYSVVGCVELSIPGRTYGLHDIAMFNLLKVMEICLHEN
                     EGNAALTYEGLLEQIRAKISHYITLMVEGSNICDIGHRDWAPVPLLSSFISDCLEKGR
                     DITDGGARYNFSGVQGIGIANLSDSLHALKGIIFDQQRLSFDELLSVLKANFTTPEGE
                     KVRARLINRFEKYGNDIDEVDNISAELLRHYCKEVEKYQNPRGGYFTPGSYTVSAHVP
                     LGSVVGATPDGRFAGEQLADGGLSPMLGQDAQGPTAVLKSVSKLDNTLLSNGTLLNVK
                     FTPATLEGEAGLRKLADFLRAFTQLKLQHIQFNVVNADTLREAQQRPQDYAGLVVRVA
                     GYSAFFVELSKEIQDDIIRRTAHQL"
     gene            complement(14183..14503)
                     /locus_tag="J1Y83_002677"
     CDS             complement(14183..14503)
                     /locus_tag="J1Y83_002677"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIB"
                     /protein_id="HBA7630816.1"
                     /translation="MTKIIAVTACPSGVAHTYMAAEALESAAKAKGWEVKVETQGSIG
                     LENELTAEDVASADMVILTKDIGIKFEERFAGKTIVRVNISDAVKRADAIMSKIEAHL
                     AQTA"
     gene            complement(14518..15597)
                     /locus_tag="J1Y83_002678"
     CDS             complement(14518..15597)
                     /locus_tag="J1Y83_002678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit EIIC"
                     /protein_id="HBA7630817.1"
                     /translation="MNELVQILKNTRQHLMTGVSHMIPFVVSGGILLAVSVMLYGKGA
                     VPDAVADPNLKKLFDIGVAGLTLMVPFLAAYIGYSIAERSALAPCAIGAWVGNSFGAG
                     FFGALIAGIIGGIVVHYLKKIPVHKVLRSVMPIFIIPIVGTLITAGVMMWGLGEPVGA
                     LTNSLTQWLQGMQQGSIVMLAVIMGLMLAFDMGGPVNKVAYAFMLICVAQGVYTVVAI
                     AAVGICIPPLGMGLATLIGRKNFSAEERETGKAALVMGCVGVTEGAIPFAAADPLRVI
                     PSIMVGSVCGAVTAALVGAQCYAGWGGLIVLPVVEGKLGYIAAVAVGAVVTAVCVNVL
                     KSLARKNGSSTEEKEDDLDLDFEIN"
     gene            15906..18407
                     /gene="ptsP"
                     /locus_tag="J1Y83_002679"
     CDS             15906..18407
                     /gene="ptsP"
                     /locus_tag="J1Y83_002679"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HBA7630818.1"
                     /translation="MALIVEFICELPNGVHARPASHVETLCNTFSSQIEWHNLRTDRK
                     GNAKSALALIGTDTLVGDNCQLLISGADEQEAHQRLSQWLRDEFPHCDAPLAEVKSDE
                     LEPLPISLTNLNPQIIRARTVCSGSAGGILTPISSLDLNALGNLPAAKGVDAEQSALE
                     NGLTLVLKNIEFRLLDSDGATSAILEAHRSLAGDTSLREHLLAGVSAGLSCAEAIVAS
                     ANHFCEEFARSSSSYLQERALDVRDVCFQLLQQIYGEQRFPAPGKLTQPAICMADELT
                     PSQFLELDKNHLKGLLLKSGGTTSHTVILARSFNIPTLVGVDIDALTPWQHQTIYIDG
                     NAGAIVVEPGESVARYYQQEARVQDALREQQRVWLTQQARTADGIRIEIAANIAHSVE
                     AQATFGNGAEGVGLFRTEMLYMDRTSAPGESELYNIFCQALESANGRSIIVRTMDIGG
                     DKPVDYLNIPAEANPFLGYRAVRIYEEYASLFTTQLRSILRASAHGSLKIMIPMISSM
                     EEILWVKEKLAEAKQQLRNEHIPFDEKIQLGIMLEVPSVMFIIDQCCEEIDFFSIGSN
                     DLTQYLLAVDRDNAKVTRHYNSLNPAFLRALDYTVQAVHRQGKWIGLCGELGAKGSVL
                     PLLVGLGLDELSMSAPSIPAAKARMAQLDSRECRQLLNQAMACRTSLEVEHLLAQFRM
                     TQQDAPLVTAECITLESDWRSKEEVLKGMTDNLLLAGRCRYPRKLEADLWAREAVFST
                     GLGFSFAIPHSKSEHIEQSTISVARLQAPVRWGDDEAQFIIMLTLNKHAAGDQHMRIF
                     SRLARRIMHEEFRNALVNAASADAIASLLQHELEL"
     gene            18419..19081
                     /gene="fsa"
                     /locus_tag="J1Y83_002680"
     CDS             18419..19081
                     /gene="fsa"
                     /locus_tag="J1Y83_002680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HBA7630819.1"
                     /translation="MELYLDTANVAEVERLARIFPIAGVTTNPSIIAASKESIWEVLP
                     RLQKAIGDEGILFAQTMSRDAQGMVKEAKRLRDAIPGIVVKIPVTSEGLAAIKMLKKE
                     GITTLGTAVYSAAQGLLAALAGAKYVAPYVNRVDAQGGDGIRTVQELQALLEMHAPES
                     MVLAASFKTPRQALDCLLAGCESITLPLDVAQQMLNTPAVESAIEKFEHDWNAAFGTT
                     HL"
     gene            19092..20195
                     /gene="gldA"
                     /locus_tag="J1Y83_002681"
     CDS             19092..20195
                     /gene="gldA"
                     /locus_tag="J1Y83_002681"
                     /EC_number="1.1.1.6"
                     /EC_number="1.1.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418380.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional L-1,2-propanediol
                     dehydrogenase/glycerol dehydrogenase"
                     /protein_id="HBA7630820.1"
                     /translation="MDRIIQSPGKYIQGADVINRLGEYLKPLAERWLVVGDKFVLGFA
                     QSTVEKSFKDAGLVVEIAPFGGECSQNEIDRLRGIAETAQCGAILGIGGGKTLDTAKA
                     LAHFMGVPVAIAPTIASTDAPCSALSVIYTDEGEFDRYLLLPNNPNMVIVDTKIVAGA
                     PARLLAAGIGDALATWFEARACSRSGATTMAGGKCTQAALALAELCYNTLLEEGEKAM
                     LAAEQHVVTPALERVIEANTYLSGVGFESGGLAAAHAVHNGLTAIPDAHHYYHGEKVA
                     FGTLTQLVLENAPVEEIETVAALSHAVGLPITLAQLDIREDVPAKMRIVAEAACAEGE
                     TIHNMPGGATPDQVYAALLVADQYGQRFLQEWE"
BASE COUNT         4869 a         5654 c         5182 g         4617 t
ORIGIN      
        1 ggtaaagaag acgcaagaat aaaaaggaat gtgacaaata atggttctgg ttgggcgtta
       61 aatttaaggt caccagaatg ctgacaaatt tctgcctgat gcggcgtaaa cgccttatcc
      121 gcctacgaag tgtcacactt tgtaggcgtg gtaagctccc cattttcagg ggagcaatat
      181 aaacaaatta ccccaaccgc gcctgcgcaa aagcaatcgc ctgcgccacc tgttgcggtg
      241 agacaccgcc ttttgctgca cgcttgtcga ggcacgattg cagcgacaga atcggataga
      301 catcttcgcc aatcacctgg ctgaatttct gcaactcact gagcggcaga tcttccaacg
      361 ctttgccctg acgaatggct tccaccacag cttcacccac aatatggtgc gcctcgcgga
      421 acggtacgcc tttcgccacc agataatccg ccagttcagt agcgttggcg taaccctgct
      481 gcgccgcttc ctggcaacgc ggacgtttca cctgaatgcc gtccagcacc agcgccgcca
      541 tatgcaggca gtccagccag gtatcgagcg cgtcgaacag accttctttg tcttcctgca
      601 tatctttgtt gtaagccagc ggcaaacctt tcagcgtcat catcatgccg gttaacgccc
      661 cctgcacccg accgcattta ccgcgaatca gctccagcgc atccgggttt ttcttctgcg
      721 gcattaatga tgaaccggaa gtcacacggt cagaaagctc cacaaacccc gcttcgccgg
      781 tgttaaagaa aatcagatct tcagcaaaac gcgacagatg caccatgccg atagcggcag
      841 cagaaagcag ttccaacaca tggtcacggt cagaaacgct gtcgagactg ttacgggtcg
      901 ccgaagcaaa gcccagccag cctgctaact gttcacggtc gatttcatag gccgttcctg
      961 ccagtgcgcc acaacctagc gggctgacat ccagacgctt aagcgcgtcc tgcaaacggc
     1021 tttcatcacg cgccagcatc tcaacgtagg ccaggcacca gtgagcgaac gtcaccggct
     1081 gggcgcgttg caggtgagtg taacccggca tcaccgcgtc ctgattgttc tgcgcggtct
     1141 cgaccagagc ggattgtaac tgccggttag ccgtcagcaa ctcgctaacg gtatctttgc
     1201 accacagttt caggtcagtt gctacctgat cattacggct acgcccggta tgcagttttt
     1261 tgcctaactg acccactttg tcgatcagtt tgccttccac ccagctatgg atatcttcgg
     1321 cgtcgctttc aaggatttgt tgtggcctgg cgcgaacatc ttccagcagc acgttcagcg
     1381 cctcttccag ttgcgcctgc tcttctgcgg ttaacacgcc gaccgttacc agggctttgg
     1441 accaggccac agagccaaca atatcctgct ccgccagacg gtaatcaaag cgcagtgagt
     1501 cgttgaattg tttgaaccgt tgatctgctg cctgggtaaa acgcccgccc caaagtgcca
     1561 taactctgtt tccttatttt gaaattcaat gccggaaagc tgcgcctccg gccaacaaaa
     1621 cttaagctaa aatccgcgta cccatcggca taccgttaaa cagtgccgga agctgttccg
     1681 catgacgcca ggaggcgata tctaccggac ggcccaacgt gcgcgcagca tccagcgccg
     1741 cgttcacttt cacaatcatg ccgtcagtaa taatgccctg ctcaatcagt tgttctgctt
     1801 tcgcggcggt catttcggca atacgttgcc ctttgccgtc gagaatgccg ctgacgtcag
     1861 agagcaaaat cagatccgcg cccagcgttg ccgccagcgc cgtcgccgcc tggtcggcat
     1921 tgacgttcat cagttgccct tcgtttgtta cgccaatgga gctgaccacc ggcagataac
     1981 cgttctccag caagctgttg ataagcttag gcgaacctgg ctgcgccagt ccaacatggc
     2041 ctaactcttc atcaagctgg gtcactttga cgctgtcgcc gtcaccgaga aacaaaccta
     2101 cggccgcaat ctgatgcttc ttcgcccatg ccagcagggt tttatttgcc gttcccgcca
     2161 gtgctccggt gataatgtct atctgatccg caggcgtcac ccgcaggcca tttttctttt
     2221 tcaccggcag attcagccct ttcatcagct catccaccac gcaaccgccg ccgtgcacaa
     2281 tcaccagctg acgctgatgt gactcgcgat aattcaccag tgcgctaaac agacgttcca
     2341 gcgcctcttc actatccagc agtacgccac ccagtttgat aattaatgga ttcatcattg
     2401 cacccttaaa taagagactg cgtttccgca tagccgaaac gaatattggc gcactgcacc
     2461 gcttgtgccg ccgcgccttt cagtaagttg tcttcggtcg ccacaataat cagatgctcg
     2521 ccctgaacgg caaaaccgat atcgcagaat ggcagtccaa cgacattttt cagcgccgga
     2581 acgcctttgt catacagccg caccagtggt ttatgggcat acgcctgctg taacgcttgc
     2641 gccacttgca cctggctcac acccggtttc aggcggcagg taatggtttc gagaatgccg
     2701 cgcgggaaat tgcccagatg cggggtgaag ataacgtcag caccgaggtg tgtggcgatc
     2761 tctggttgat ggcgatgggt aaagacgcca tacggttgca ggctaacttc acaaaaacta
     2821 tttgaaatcg ctgccttacg tcctgcaccg ctcacgccgc tggtggcgtt gatcaccggc
     2881 cactgattga ggtcaagaag atcggcatca atcaacggtt tcagcgccag ctgtgctgcc
     2941 gtcggataac agcctggcac ggcaatcaag ttcgcttctt ttaatttatt accgcaccac
     3001 tccgccagac cgtaggctgc ctgttccagc agttccgggt attgatgggt aaagccgtaa
     3061 tatttttcat agaaggcgac atcgttaaca cgaaacgcgc cggaaaggtc gaacaccaca
     3121 cagcctgctt caagaaattg cggcgctaaa tcgtggctga cttcatgggc ggtggcgaga
     3181 aacaccacgt ccacccctgg gctaaactcg ctgatatccg acatcggctg caacggcaga
     3241 tcaacgatgc cttttagctg cggatgcaaa tcggagatta actttcccgc atcattactt
     3301 tgcgctgaaa cagtcaaagc ggttatgttc atatgcggat ggcgatttac ataggtcact
     3361 agctctgcgc cagcgtagcc gctggcaccc acaatcagcg tattcaacat cggggctatt
     3421 caccttctta tgtctggttg ccaggttaaa cgtaaaacat tcaccttacg gctggtgggt
     3481 tttattacgc tcaacgttag tgtattttta ttcataaata ctgcatgaat attgatacta
     3541 tcatgaccag aggtgtgtca acaatgaaaa acaaattacc gccatttatc gagatttacc
     3601 gtgctctgat tgccacacct tcaataagcg ccacggaaga ggcactcgat caaagcaatg
     3661 cagatttaat cactctgctg gcggactggt ttaaggattt aggcttcaat gtggaagtac
     3721 agcctgtgcc aggaactcgc aacaaattta atatgctggc cagttgcgga cagggggctg
     3781 gcggtttgtt gctggcgggg cataccgata cggtgccatt tgatgacgga cgctggacgc
     3841 gcgatccatt tacgttgacg gagcatgacg gcaagcttta cggcttaggc accgccgaca
     3901 tgaaaggctt ttttgcgttt atccttgatg cgctacgcga tgtcgacgtc acgaagcttg
     3961 caaaaccgct ctacattctg gcgactgccg atgaagaaac cagtatggcc ggagcgcgtt
     4021 attttgccga aactaccgcc ctgcgcccgg attgcgccat cattggcgag ccaacgtcgc
     4081 tgcaaccggt acgcgcgcat aaaggtcata tctctaacgc catccgtatt cagggccagt
     4141 cgggccactc cagcgatcca gcacgcggag ttaacgctat cgaactgatg cacgacacca
     4201 tagggcatat tttgcaattg cgcgataacc tgaaagaacg ttatcactac gacgcgttta
     4261 ccgtgccata ccctacgctc aacctcgggc atatccacgg tggcgacgct tctaaccgta
     4321 tttgtgcttg ctgtgagttg catatggata ttcgtccgct gccaggcatg acactcaatg
     4381 aacttaatgg tttgctcaac gatgcattgg ctccagtgag cgaacgctgg ccgggtcgtc
     4441 tgacggtcga cgagctgcat ccgccgatcc ctggctatga atgcccaccg aatcataaac
     4501 tggttgaagt ggttgagaaa ttgctcggag caaagaccga agtggtgaat tactgtaccg
     4561 aagcgccgtt tattcaaacg ttatgcccga cgctggttct ggggcctggc tcaattaatc
     4621 aggctcatca gcctgatgaa tatctggaga cgaggtttat caagcccacc cgcgaactga
     4681 tcacccaggt gattcaccat ttttgctggc attaaaacgt aggccaaaca atgtcggatg
     4741 cgatgcttac gcatcttatc cgacctacac ctttggtgtt actcgaggcg attttttaac
     4801 attcccataa gttacgctta tttaaggagt cgtgaattta atggcgtaaa ttcctgctat
     4861 ttattcgttt gctgaagcga tttcgcagca tttgacgtca tcgcttttac gtggctttat
     4921 aaaagacgac gaaaagcaaa gcccgagcat attcgcgcca atgcgacgtg aaggatacag
     4981 ggctatcaaa cgataagatg gggtgtctgg ggtaatatga acgaacaata ttccgcattg
     5041 cgtagtaatg tcagtatgct cggcaaagtg ctgggagaaa ccatcaagga tgcgttggga
     5101 gaacacattc ttgaacgcgt agaaactatc cgtaagttgt cgaaatcttc acgcgctggc
     5161 aatgatgcta accgccagga gttgctcacc accttacaaa atttgtcgaa cgacgagctg
     5221 ctgcccgttg cgcgtgcgtt tagtcagttc ctgaacctgg ccaacaccgc tgagcaatac
     5281 cacagcattt cgccgaaagg cgaagctgcc agcaacccgg aagtgatcgc ccgcaccctg
     5341 cgtaaactga aaaaccagcc gcaactgagc gaagacacca tcaaaaaagc agtggaatcg
     5401 ctgtcgctgg aactggtcct cacggctcac ccaaccgaaa ttacccgtcg tacactgatc
     5461 cacaaaatgg tggaagtgaa cgcctgttta aaacagctcg ataacaaaga tatcgccgac
     5521 tacgaacaca accagctgat gcgccgcctg cgccagttga tcgcccagtc atggcatacc
     5581 gatgaaatcc gtaaactgcg cccaagcccg gtagatgaag ccaaatgggg ctttgccgta
     5641 gtagaaaaca gcctgtggca aggcgtaccg aattacctgc gcgaactaaa cgaacaactg
     5701 gaagagaacc tcggctacaa actgcccgtc gaatttgttc cggtacgttt tacctcgtgg
     5761 atgggcggtg accgcgacgg caacccgaac gtcactgccg atatcacccg ccacgtcctg
     5821 ctactcagcc gctggaaagc caccgatttg ttcctgaaag atattcaggt tctggtttct
     5881 gaactgtcga tggttgaagc cacccctgaa ctgctggcgc tggttggcga agagggtgcc
     5941 gcagaaccgt atcgctatct gatgaaaaac ctgcgttctc gcctgatggc gacacaggca
     6001 tggctggaag cgcgcctgaa aggcgaagaa ctgccaaaac cagaaggtct gctgacacaa
     6061 aacgaagaac tgtgggaacc gctctacgct tgctaccagt cacttcaggc gtgtggcatg
     6121 ggtattatcg ccaatggcga tctgctcgac accctgcgcc gcgtgaaatg tttcggcgta
     6181 ccgctggtgc gtattgatat ccgtcaggag agcacccgcc ataccgaagc gctgggcgag
     6241 ctgacccgct acctcggtat cggcgactac gagagctggt cagaagccga caaacaggcg
     6301 ttcctgatcc gcgaactgaa ctccaaacgt ccgcttctac cgcgcaactg gcaaccaagc
     6361 gccgaaacgc gcgaagtgct cgatacctgc caggtgattg ccgaagcgcc gcaaggctcc
     6421 attgccgcct acgtgatctc gatggcgaaa acgccgtccg acgtgctggc tgtccacctg
     6481 ctgctgaaag aagcgggtat cgggtttgcg atgccggttg ctccgctgtt tgaaaccctc
     6541 gatgacctga acaacgccaa cgatgtcatg acccagctgc tgaatatcga ctggtatcgc
     6601 ggcctgattc agggcaaaca gatggtgatg attggctatt ccgactcagc aaaagatgcg
     6661 ggcgtgatgg cagcttcctg ggcgcaatat caggcacagg atgcattaat caaaacctgc
     6721 gaaaaagcgg gtattgagct gacgttgttc cacggtcgcg gcggttccat tggtcgcggc
     6781 ggcgcacctg cccatgcggc gctgctgtca caaccgccag gaagcctgaa aggtggcctg
     6841 cgcgtgaccg aacagggcga gatgatccgc tttaaatatg gtctgccaga aatcaccgtc
     6901 agcagcctgt cgctgtacac cggggcgatt ctggaagcca acctgctgcc accgcctgag
     6961 ccgaaagaaa gctggcgtcg cattatggat gaactgtcag tcatctcctg cgatctctac
     7021 cgtggctacg tacgtgaaaa caaagatttt gtgccttact tccgctccgc tacgccggaa
     7081 caagaattgg gtaaactgcc gttgggttca cgtccggcga aacgtcgccc gaccggcggc
     7141 gtcgagtcgc tgcgcgccat tccgtggatt tttgcctgga cgcaaaaccg cctgatgctc
     7201 cccgcctggc tgggtgcagg tacggcgctg caaaaagtgg tcgaagatgg caaacagagc
     7261 gagctggaag ccatgtgccg cgattggcca ttcttctcga cgcgtctcgg catgctggag
     7321 atggtcttcg ccaaagcaga cctgtggctg gcggaatact atgaccaacg cctggtagac
     7381 aaagcactgt ggccgttagg taaagagtta cgcaatctgc aagaagaaga catcaaagtg
     7441 gtgctggcga ttgccaacga ttctcacctg atggccgatc tgccgtggat tgcagagtct
     7501 attcagctac ggaatattta caccgacccg ctgaacgtat tgcaggccga gttgctgcac
     7561 cgctcccgcc aggcagaaaa agaaggccag gaaccggatc ctcgcgtcga gcaggcgtta
     7621 atggtcacta ttgccgggat tgcggcaggt atgcgtaata ccggctaatc ttcctctcct
     7681 gcaaaccctc gtgcttttgc gcgagggttt tctgaaatac ttctgttcta acaccctcgt
     7741 tttcaatata tttctgtctg cattttattc aaaatctgga tatcccttca gatatcctta
     7801 aggaattgtc gttacattcg gcgatatttt tttcaagaca ggttcttact atgcattcca
     7861 cagaagtcca ggctaaaccc ctttttagct ggaaagccct gggttgggca ctgctctact
     7921 tttggttttt ctctactctg ctacaggcca ttatttacat cagtggttat agtggcacta
     7981 acggcattcg cgactcgctg ttattcagtt cgctgtggtt gatcccggta ttcctctttc
     8041 cgaagcggat taaaattatt gccgcagtga tcggcgtggt gctatgggcg gcctctctgg
     8101 cggcgctgtg ctattatgtc atctacggtc aggagttctc gcagagcgtt ctgtttgtga
     8161 tgttcgaaac caacaccaac gaagccagcg agtatttaag ccagtatttc agcctgaaaa
     8221 ttgtgctcat cgcgctggcc tatacggcgg tggcagttct gctgtggaca cgcctgcgcc
     8281 cggtctatat tccaaagccg tggcgttatg ttgtctcttt tgccctgctt tatggcttga
     8341 ttctgcatcc gatcgccatg aatacgttta tcaagaacaa gccgtttgag aaaacgttgg
     8401 ataacctggc atcgcgtatg gagcctgccg caccgtggca attcctgacc ggctattatc
     8461 agtatcgtca gcaactaaac tcgctaacaa agctactgaa tgaaaataat gccttgccgc
     8521 cgctggctaa tttcaaagat gaatcgggta acgaaccgcg caccttagtg ctggtaattg
     8581 gcgaatcgac ccagcgtgga cgcatgagtc tgtacggtta tccgcgtgaa accacgccgg
     8641 agctggatgc gctgcataaa accgatccga atctgaccgt gtttaataac gtggttacgt
     8701 ctcgtccgta caccattgaa atcctgcaac aggcgctgac ctttgccaat gaaaagaacc
     8761 cggatctgta tctgacgcag ccgtcgctga tgaacatgat gaaacaggcg ggttataaaa
     8821 ccttctggat caccaaccag cagacgatga ccgcccgcaa taccatgctg acggtatttt
     8881 cgcgccagac cgataagcag tactacatga accagcaacg tacgcagagt gcgcgtgaat
     8941 acgacaccaa cgtgctgaag ccgttccagg aagtgctgaa ggaccctgcg ccgaagaaac
     9001 tgatcatcgt tcatctgctg ggtacgcata tcaagtacaa gtaccgttat ccggaagatc
     9061 agggcaaatt tgacggtaat acagagcatg ttccaccggg attaaacgca gaagagctgg
     9121 agtcatataa tgattatgac aatgctaacc tgtacaacga tcacgtggtt gccagcctga
     9181 ttaaagactt taaagcgacc gatccgaacg gtttcctggt ttatttctct gaccacggtg
     9241 aagaggttta cgacacgccg ccgcacaaaa ctcaggggcg taatgaagac aacccgacgc
     9301 gtcatatgta caccattccg ttcctgctgt ggacgtcaga aaaatggcaa gcgactcatc
     9361 cgcgtgattt ctcacaggat gttgatcgta aatacagcct ggcggaactg atccacacct
     9421 ggtcagattt ggcgggctta tcttacgacg gttacgatcc aacccgttca gtggtgaatc
     9481 cgcagttcaa agaaactacc cgctggattg gtaacccgta taagaaaaac gcactgatcg
     9541 attacgacac tctgccgtat ggcgatcagg tgggtaatca gtaattatca gccagatgtt
     9601 atttgccgga tgcgacgctt gacgcgtctt atccggccta cagatcgcgg cggtaggccg
     9661 gataaggcgt ttacgccgca tccggcaata caagtcgact gaaactgccg caatgtttac
     9721 atctcactcc catcaggcca cacttttcag gtggcctttt ttcataatgc cagttactgt
     9781 tttcccgtct acatacctaa gggaataaca tgtatcacga cgtcagctat ctgctctccc
     9841 gcctgatcaa cggcccgctg tccctgcgcc agatttactt tgccagcagc aacggcccgg
     9901 tgcccgatct tgcgtatcag gtcgattttc cacggctgga aattgtgctg gaaggtgagt
     9961 ttattgatac cggcgctaga gcagcgttag ttcccggcga tgtgctgtac gtccctgctg
    10021 gtggctggaa ttttccacaa tggcaagccc ccgccaccac ctttagcgtg ctgtttggca
    10081 aacagcaact cggcttcagc gttgtgcaat gggatggcaa acaatatcaa aatctggcga
    10141 agcaacacgt cgcccgacgc ggcccgcgca ttggttcttt tctgctacaa acgctcaatg
    10201 aaatgcaaat gcagtcgcag gagcagcaaa cggccagact tatcgtcacc agcctgctta
    10261 gccactgccg cgatttgctc ggcagccaaa tacagaccgc ctcacgcagc caggctctat
    10321 tcgaagctat tcgtgattat atcgacgaac gctatgcctc cgcacttacc cgcgaatctg
    10381 tcgcacaggc gttctatatt tcgccaaatt acctctcgca cctgtttcaa aaaacggggg
    10441 ccattggttt taacgaatac ctgaatcaca cgcgactgga gcacgctaag acattattga
    10501 aaggctatga cctgaaggta aaagaagtgg cgcacgcctg cggctttgtc gacagcaact
    10561 acttctgccg tctgtttcgt aaaaataccg aacgctcgcc gtcggaatat cgtcggcagt
    10621 accacagcca actgacagaa aaaccgacta ctccagaata agatgggttt gctgcggcgc
    10681 agaaagcact ttgcgcactg cgctcatcac cctctgcggc tcacgcagga aagcgtagat
    10741 actgcattgc acgtagcgac aatgttcaaa acgctcggca ccggcaagct cgatatccgt
    10801 aattaacagc gcaacatccg cccggcggat atcctcgtcc gctaaacgat tctccgttcc
    10861 caacgcgccc tgagtttcaa tgctgacgcc ccacttctct aactggcaca acttttccag
    10921 ccgttccgcc gccatataag tatgcgccac gccgcttacg caggcggtta ctgccaccag
    10981 atatgccatt ttaacctccc acggtaacct gaaatccggc ccgttctgcc atttcacgca
    11041 tcgttgccac atcggctgac gacggcgcag caacctcttt catcgaccat gttttcccca
    11101 gcaggcggta tttcggctcg ccgtactgat gaaacggtaa cagatggatc tgcttgatat
    11161 tcagcgggat cagcacgtcc agcgcctgct gcatattctc ccggctgagc gtgaaaccag
    11221 ggatcagcgg taaacgcggg atcacgttga caccctcact caccagcaaa cgcagattct
    11281 ccagcacgcg tggcagattc atcttcacca catcccgcgc ctgagccgca tccataattt
    11341 ttaaatcgaa caacacttca tcgcacaatt tcgccagcgg taacagcttg ctggctggcg
    11401 cgtctccggc agtttcgatg gcgcatgaca caccccatag ccgcagtcgc tgtaaaaaac
    11461 gggtcgcaaa ctccgcctgc attaacactt cgccgccaga aagcgtgacg ccaccgccgg
    11521 acgtgcgaaa aaaaatgtca tctttcatca cttcccgttc cagcgcgtca aggctgatat
    11581 cgcggccaat ccgttcaaat gccccggagg ggcattcatc cgcatcacgc aaacatttcg
    11641 cgcagtgcag acatttcgcc tctctgcgca ccgtctggat tttgccggag atcgactccg
    11701 gattagcaca ccatgggcaa agatgcggac agcctttaaa aaagaccacc gtacgaatgc
    11761 cctcgccgtc attcagtgaa taacgctgaa tgttgaaaat gcgcgccaca tcatcgcggc
    11821 gcgtttccac aacgttacag ctgatgcgct gtccggcgga tgatgtcatc ctggatctcc
    11881 ttcgacagtt cgacaaagaa ggcgctgtat ccggcaacgc gcaccaccag cccggcataa
    11941 tcttgtgggc gctgttgcgc ttcccgcaac gtgtcggcgt tcaccacgtt aaactgaata
    12001 tgctgcaact taagctgggt aaacgcccgt aagaagtcgg ccagtttgcg taatcccgct
    12061 tcaccttcca gggtcgccgg agtgaatttc acgttcagca acgtaccgtt agacagcagc
    12121 gtattatcga gcttactgac tgacttcagt accgctgttg gcccttgtgc gtcctgtccc
    12181 agcatgggtg acaagccgcc gtctgccagc tgttctccgg caaaacgacc gtctggcgtc
    12241 gcgccaacca ccgatcccaa cgggacgtga gcagaaacgg tatacgatcc tggcgtgaag
    12301 tagccgccgc gcgggttctg gtatttttcc acttctttgc agtagtggcg taacagttcg
    12361 gcgctgatgt tatccacctc gtcgatatcg ttaccgtatt tctcgaagcg gttaattaag
    12421 cgagcgcgga ctttttcgcc ttctggcgtt gtgaagttgg cttttaatac cgacagcaat
    12481 tcgtcaaaac ttaaacgctg ttgatcaaaa attatccctt tcaacgcatg gagagaatcg
    12541 ctcaggttgg cgataccgat cccctgtacg ccggagaagt tataacgcgc gccgccatcg
    12601 gtaatatcgc ggcctttttc cagacaatcg ctgataaaag acgagagtag cggtacagga
    12661 gcccagtcgc gatggccgat atcacaaata ttgctgccct caaccatcag ggtgatgtag
    12721 tggctgatct tggcgcggat ctgttccagt aaaccttcat aagtcagcgc agcgttgcct
    12781 tcattttcat gcaggcagat ttccatcact ttcagcaggt taaacatcgc gatgtcatgc
    12841 aagccgtagg ttctgccggg aatagataat tccacacagc ccactacgga atagtcgcgc
    12901 gcatcttcca gcgaaacgcc acggttgagg aacgctggca ccaccacttc atcgttaaag
    12961 atttgcggaa taccggtgcc gaggcgaatg gtttctgcag ttttcatcag gaacggcgtg
    13021 tcgatcaagg cgttagtgcg cacgccgagg ttcggttgcg gtaattgcac gctttgataa
    13081 gcgtcaaggc aaaggaacga aagcacgttc accgcgctac gtccgttctc ggttaacccg
    13141 ccgagcagtg cggtatagcc ggtagggaaa ccggcgaaat aacgcgcgct actggtggag
    13201 cgcaacagca cgatgtcgtt gcatttcacc cataaagatt cgagcagttc tttcaggaat
    13261 gccggatctt cgccctgggt taatgatgcc tgatagaacg gcaacatata ctggtcgaag
    13321 cgccccaacg atagcgaact ggcgttggat tcgtattgca gaatgatgtt catgtaccag
    13381 aataactggc acgcctgcca gaacgtctgc ggcttatgtt cggcgttatg gcgggagatc
    13441 tccgcaatgg tcagcagttc ttcgcgacgc tggccatcag tgcagcttgc cgccatcgtt
    13501 tccgccagtt cggcgtaacg caaaatatgt ttctgcgagg cttccagcag taacagcgct
    13561 gcctgataaa agtgattctc cggctgttgc tgacaatgtt gctgcatctg tgccaccagc
    13621 tcccccagcc cgtgattcag caggcgcggg taatcaataa taatgtgccc ctggccttta
    13681 tccgtctggt tgatgctgaa aatctgcgtg ctggtcgcgg cttttacttc atccgtcatc
    13741 tgcccgttga tgaaatcttt catcgaacgt ttttcccagt acgggaacaa ctcttcgcgg
    13801 tagatacgtt tatcttcttc gctgatagca aagcggtcct gcggacgcgt cgggaattga
    13861 tccagctctt tcagcagcca gtaagggtcc atttccggcg acataatccc ggcgcgcggt
    13921 tttacggtgc ggttaccggc aatcagttct tcatcacgaa tcgaaatttc aacatgttca
    13981 aggatatacg ctgttgcttt cgcccgacgc attatcaccg gctccccttc ggtttgccga
    14041 tggctggcgg tataaagcaa cgcccgctcc agcgagattt cacgggtatt ggcaaacagt
    14101 gcagttttga ggcgagagat acgattcgtc atgaggaact cctgtgggaa agcgaacccg
    14161 cccttgcggg cgggataaga acttaagcgg tttgtgccag gtgagcttca attttgctca
    14221 taatggcgtc agcacgcttt accgcgtcgc tgatgttgac acgcacgata gtcttgccag
    14281 caaaacgctc ttcaaatttg atgccgatat ctttggtcaa aatcaccata tcggcgcttg
    14341 ccacgtcttc cgcagtcagt tcattttcca gaccaatcga cccctgggtt tctactttca
    14401 cttcccagcc tttcgctttc gcggcgcttt ccagcgcctc tgccgccata taagtatggg
    14461 caacacctga aggacatgcg gttactgcga taattttcgt catggctcgt tcctcaatta
    14521 attaatttca aaatccagat ccaggtcgtc ttccttctct tcagttgaag acccattttt
    14581 acgcgccaga cttttcagca cgttcacgca gactgccgtc acaaccgccc cgacagccac
    14641 tgccgcgatg taacccaact taccttcaac caccggcagc acaatcagac caccccagcc
    14701 tgcatagcac tgcgcaccga ccagcgccgc agttaccgca ccacaaacag aaccgaccat
    14761 gatggaagga atgacacgca acggatcggc tgcggcgaaa ggaatcgccc cttcagtaac
    14821 gccaacacag cccatcacca gtgccgcctt gccggtttcg cgctcttctg cggagaaatt
    14881 tttacgacca atcagcgtcg ccagccccat ccccagcggc gggatacaaa tacccacggc
    14941 agcaatggcc accacggtat aaacaccctg agcaacgcaa atcagcatga aggcataggc
    15001 cactttgtta accgggccgc ccatatcgaa cgccagcatc agacccatga tcaccgccag
    15061 cataacaatg ctgccctgct gcatcccctg aagccactgg gtcaggctgt tggtcaacgc
    15121 ccccaccggc tcgcccagcc cccacatcat gacgccagcg gtaatcagtg tgccgacgat
    15181 tggaatgatg aagattggca tcaccgagcg cagaacttta tgcaccggaa ttttcttcag
    15241 gtaatgcacc acgatgccgc cgataatccc ggcgatcagc gcaccaaaga agcccgcgcc
    15301 aaagctgtta ccaacccagg caccgatagc gcatggagcc agcgcagaac gttctgcaat
    15361 ggaataaccg atgtaagcgg cgaggaaagg caccatcagc gtcaaacctg caacgccgat
    15421 atcaaacagt tttttcagat tcggatcggc tacggcatcc ggcactgcgc ctttgccata
    15481 caacatgacg gaaaccgcca gcaaaatacc gcccgatacc acgaagggaa tcatgtgtga
    15541 aacgcccgtc attaaatgct gacgggtatt tttcaggatc tgcaccaact cattcataag
    15601 ctgctcccgg gcttgcgaaa gcccatgtcc atatttttga tggaagaaac aataggcaat
    15661 cgcaggcgga gcaaacagtg gataaaaagg ccatttactg gacttttgta atatcagtac
    15721 aaaaatgcga tccgcctcat aacttgcgat aatccggaag tacgccgcaa aacggcgtct
    15781 tgtgagacgc gtcgcaaatt gacggttaca ttacatttgt ccagcttttg gcagatttgt
    15841 cacatggcag gccgttgtcg tgcgctctta tcctgtaaac agaaaaccgt accgggagaa
    15901 aggcaatggc cctgattgtg gaatttattt gtgagctacc taacggcgtg catgcgcgtc
    15961 cggcaagcca cgttgaaacg ctgtgtaata ctttttcatc acaaattgag tggcataacc
    16021 tgcgcactga ccgcaagggc aacgccaaaa gcgcccttgc gctgattggc accgatacgc
    16081 tggtgggcga taactgccag ttactgattt ccggggccga cgaacaggaa gcgcaccagc
    16141 gtttaagcca gtggctgcgc gatgaattcc cccactgcga cgcaccgctg gcggaagtaa
    16201 aatctgacga actggaacca ctgccgattt cactgaccaa tctgaatccg caaattatcc
    16261 gtgctcgcac cgtgtgcagc ggtagcgcag gcggcattct gacgccgatc tcttctttag
    16321 atctcaatgc gctgggtaat cttcccgcag ccaaaggcgt tgacgccgaa caatccgcac
    16381 tggaaaacgg tctgacgctg gtactgaaaa acatagagtt tcgtctgctg gatagcgacg
    16441 gtgctaccag cgcgattctg gaagcgcacc gatccctggc tggcgatact tccctgcgcg
    16501 aacatttact ggcaggtgtc agcgccggat taagctgcgc cgaagcgatt gttgccagtg
    16561 cgaatcactt ttgcgaagag ttcgcccgtt ccagcagcag ctacctgcaa gaacgtgccc
    16621 tggacgtacg cgacgtctgc ttccagttac tccagcaaat ctacggtgag caacgcttcc
    16681 cggcaccggg caaactgacg cagcccgcca tttgtatggc tgatgaactg acccccagcc
    16741 agttcctcga actggataaa aatcacctca aaggtctatt actgaaaagt ggcggcacca
    16801 cctcacatac ggtgatcctt gcccgttcgt tcaacattcc gacgctggta ggtgtggata
    16861 ttgatgccct tactccgtgg caacatcaaa cgatttatat cgacggcaac gcaggggcga
    16921 ttgtggttga gccaggggaa tccgtggccc gttattatca gcaagaagcc cgcgtacagg
    16981 acgctctgcg tgagcaacag cgtgtctggc tgacccagca agcgcgtacc gcagacggta
    17041 tccgcattga aattgccgct aacatcgctc actccgtgga agcgcaggcc acattcggca
    17101 atggtgcgga aggcgttggt ttgttccgca ctgaaatgct ctatatggat cgcaccagtg
    17161 caccgggtga aagcgagctg tacaacattt tttgtcaggc gctggagtct gccaacggac
    17221 gcagcattat tgtgcgcact atggacattg gcggtgataa acccgttgat tatctgaaca
    17281 ttccggcaga agctaacccg ttcctcggtt atcgcgcggt gcgtatttat gaagaatacg
    17341 cgtcgttgtt taccacacaa ctacggtcga tcctgcgcgc ctctgctcac ggcagcctga
    17401 aaatcatgat cccgatgatc tcctcaatgg aagagatctt atgggtgaaa gaaaaactgg
    17461 cggaagccaa acagcaactg cgtaacgaac acattccgtt tgatgagaag atccagctcg
    17521 gcatcatgct ggaagtgccg tcggtgatgt tcatcatcga tcaatgctgc gaagagattg
    17581 atttctttag tattggtagt aatgacctga cgcaatatct actggcagtg gatcgcgata
    17641 acgctaaggt gactcgtcac tacaacagcc tgaatccggc cttcttgcgg gcgctcgatt
    17701 acaccgtgca ggcggtgcat cgccagggca aatggattgg tctgtgcggt gaactgggag
    17761 cgaaaggttc cgtgctgccg ttgctggtcg gtttagggct ggatgaactc agcatgagcg
    17821 caccatcaat tccggcggcg aaagcgcgga tggcgcaact tgatagccgt gagtgccgcc
    17881 agttgctcaa ccaggcaatg gcctgccgta cttcgctgga agtggaacac ctgctggcgc
    17941 aattccgcat gacccaacag gacgcaccgc tggtcaccgc cgagtgcatc acactggaaa
    18001 gcgactggcg cagcaaagaa gaagtgctca aaggcatgac cgataacctg ctgctggcgg
    18061 gccgctgccg ctatccgcgt aaactggaag ccgacttgtg ggcgcgcgag gccgttttct
    18121 ctaccggact gggctttagt ttcgccattc cccacagtaa atcggaacac attgagcaat
    18181 ccaccatcag cgtagcgcgt ctgcaagcgc cagtgcgctg gggcgatgat gaagcgcaat
    18241 tcatcattat gttaaccctg aacaaacacg ccgcgggcga tcaacatatg cgcattttct
    18301 cgcgcctcgc tcgtcgcatc atgcacgaag aattccgtaa tgcgctggtt aacgccgcct
    18361 ctgccgacgc tatcgccagc ctgctgcaac atgaactgga actgtaaaag gaaacatcat
    18421 ggaactgtat ctggacaccg ctaacgtcgc agaagtcgaa cgtctggcac gcatattccc
    18481 gattgccggg gtgacaacta acccgagcat tatcgctgcc agcaaggagt ccatatggga
    18541 agtgctgccg cgccttcaaa aagcgatcgg tgatgagggc attctgtttg ctcagaccat
    18601 gagccgcgac gcgcagggta tggtgaaaga agcgaaacgc ctgcgcgacg ctattccggg
    18661 cattgtggtg aaaattccgg taacctctga aggtctggca gcaattaaaa tgctgaagaa
    18721 agaaggcatt actacgctgg gaaccgcagt gtacagcgcc gcgcaaggat tactggcggc
    18781 gctggctgga gccaaatacg ttgctccata cgttaaccgc gtagatgccc agggcggtga
    18841 cggcattcgt actgtacagg agttgcaagc gttactggaa atgcatgcgc cagaaagcat
    18901 ggtgctggct gccagcttta aaacaccacg tcaggcgctg gattgtttgc tggctggatg
    18961 tgaatccatc acactgccct tagatgtagc gcaacaaatg cttaacaccc ctgcggtaga
    19021 gtcagctata gagaagttcg agcacgactg gaatgccgca tttggcacta ctcatctcta
    19081 aaggagtaat tatggaccgc attattcaat caccaggtaa atacatccag ggcgcagatg
    19141 taattaatcg tctgggcgag tacctgaagc cactggcgga acgctggtta gtggtgggtg
    19201 acaaatttgt tttaggtttt gctcaatcca ctgtcgagaa aagctttaaa gatgctggac
    19261 tggtagtaga aattgcgccg tttggcggtg aatgttcgca aaatgagatc gaccgtctgc
    19321 gtggcatcgc ggagactgcg cagtgtggcg caattctcgg tatcggtggc ggaaaaaccc
    19381 tcgatactgc caaagcactg gcacatttca tgggtgttcc ggtagcgatc gcaccgacta
    19441 tcgcttctac cgacgcaccg tgcagcgcat tgtctgttat ctacaccgat gagggtgagt
    19501 ttgaccgcta tctgctgttg ccaaataacc cgaatatggt cattgtcgac accaaaatcg
    19561 tcgctggcgc acctgcacgt ctgttagcgg cgggtatcgg cgatgcgctg gcaacctggt
    19621 ttgaagcgcg tgcctgttcc cgtagcggcg cgaccaccat ggcgggtggc aagtgcactc
    19681 aggcagcgct ggcactggct gaactgtgct acaacaccct gctggaagaa ggcgaaaaag
    19741 cgatgcttgc cgccgaacag catgtagtga ctccagcgct ggagcgcgtg attgaagcga
    19801 acacctattt gagtggcgtt ggttttgaaa gcggcgggct ggcggcggca cacgcggtac
    19861 ataacggcct gaccgctatc ccggacgcgc atcactatta tcatggtgaa aaagtggcat
    19921 tcggtacgct gacgcagctg gtgctggaaa acgcgccggt ggaggaaatc gaaaccgtag
    19981 ctgcgcttag ccatgcggta ggtttgccaa taactctcgc tcaactggat attagagaag
    20041 atgtcccggc gaaaatgcga attgtggcag aagcggcatg tgcagaaggt gaaaccatcc
    20101 acaacatgcc tggcggcgcg acgccagatc aggtttacgc cgcactgctg gtagccgacc
    20161 agtatggtca gcgtttcctg caagagtggg aataacctac tccaaactcc cggcttgtcc
    20221 gggagtttga acgcaaaatt gcctgatgcg ctacgcttat caggcctacg caatctctgc
    20281 aatatagtaa atttacgtac ttttgtaggc cggataaggc gt
//
LOCUS       DADSYM010000086        20214 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2415
            SAMN08039579-rid11723173.denovo.086, whole genome shotgun sequence.
ACCESSION   DADSYM010000086 DADSYM010000000
VERSION     DADSYM010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039579
            Sequence Read Archive: SRR6362533
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 20214)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20214)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980162.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 03:39:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,484
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 4,347
            CDSs (with protein)               :: 4,347
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 43 of 85
            Pseudo Genes (incomplete)         :: 37 of 85
            Pseudo Genes (internal stop)      :: 19 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20214
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039579-rid11723173.denovo.086"
                     /strain="LGE2415"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8302 N 95.9628 W"
                     /collection_date="2015-08-24"
     gene            510..2009
                     /gene="ansP"
                     /locus_tag="J1Y83_002682"
     CDS             510..2009
                     /gene="ansP"
                     /locus_tag="J1Y83_002682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310084.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-asparagine permease"
                     /protein_id="HBA7630821.1"
                     /translation="MSKHNTDTSDQHAAKRRWLNAHEEGYHKAMGNRQVQMIAIGGAI
                     GTGLFLGAGARLQMAGPALALVYLICGLFSFFILRALGELVLHRPSSGSFVSYAREFL
                     GEKAAYVAGWMYFINWAMTGIVDITAVALYMHYWGAFGGVPQWVFALAALTIVGTMNM
                     IGVKWFAEMEFWFALIKVLAIVTFLVVGTVFLGSGQPLDGNTTGFHLITDNGGFFPHG
                     LLPALVLIQGVVFAFASIEMVGTAAGECKDPQTMVPKAINSVIWRIGLFYVGSVVLLV
                     MLLPWSAYQAGQSPFVTFFSKLGVPYIGSIMNIVVLTAALSSLNSGLYCTGRILRSMA
                     MGGSAPSFMAKMSRQHVPYAGILATLVVYVVGVFLNYLVPSRVFEIVLNFASLGIIAS
                     WAFIIVCQMRLRKAIKEGKAADVSFKLPGAPFTSWLTLLFLLSVLVLMAFDYPNGTYT
                     IAALPIIGILLVIGWFGVRKRVAEIHSTAPVVEEDEEKQEIVFKPETSS"
     gene            complement(2122..3183)
                     /locus_tag="J1Y83_002683"
     CDS             complement(2122..3183)
                     /locus_tag="J1Y83_002683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YncE family protein"
                     /protein_id="HBA7630822.1"
                     /translation="MHLRHLFSSRLRGSLLLGSLLVASSFSTQAAEEMLRKAVGKGAY
                     EMAYSQQENALWLATSQSRKLDKGGVVYRLDPVTLEVTQAIHNDLKPFGATINNTTQT
                     LWFGNTVNSAVTAIDAKTGEVKGRLVLDDRKRTEEVRPLQPRELVADDATNTVYISGI
                     GKESVIWVVDGENIKLKTAIQNTGKMSTGLALDSKGKRLYTTNADGELITIDTADNKI
                     LSRKKLLDDGKEHFFINISLDTNNQRAFITDSKAAEVLVVDTRNGNILAKVAAPESLA
                     VLFNPARNEAYVTHRQAGKVSVIDAKSYKVVKTFDTPTHPNSLALSADGKTLYVSVKQ
                     KSTKQQEATQPDDVIRIAL"
     gene            3425..5527
                     /locus_tag="J1Y83_002684"
     CDS             3425..5527
                     /locus_tag="J1Y83_002684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HBA7630823.1"
                     /translation="MKIFSVRQTVLPALLVLSPVVFAADEQTMIVSAASQVVSELDTP
                     AAVSVVDGEEMRLATPRINLSESLTGVPGLQVQNRQNYAQDLQLSIRGFGSRSTYGIR
                     GIRLYVDGIPATMPDGQGQTSNIDLSSVQNVEVLRGPFSALYGNASGGVMNVTTQTGQ
                     QPPTIEASSYYGSFGSWRYGLKATGATGDGTQPGDVDYTVSTTRFTTHGYRDHSGAQK
                     NLANAKLGVRIDDASKLSLIFNSVDIKADDPGGLTEAEWKANPQQAPRAEQYDTRKTI
                     KQTQAGLRYERSLSAQDDMSVMMYAGERETTQYQSIPMAPQLNPSHAGGVITLQRHYQ
                     GIDSRWTHRGELGVPVTFTTGLNYENMSENRKGYNNFRLNRGVPEYGQKGELRRDERN
                     LMWNVDPYLQTQWQLTDKLSLDAGVRYSSVWFDSNDHYVTPGNGDDSGDASYHKWLPA
                     GSLKYAMTDAWNVYLAAGRGFETPTINELSYRADGQSGMNFGLKPSTNDTIEIGSKTR
                     IGDGLLSLALFQTDTDDEIVVDSSSGGRTTYKNAGKTRRQGAELAWDQRFAGDFRVKA
                     SWTWLDATYRSNVCNEQDCNGNRMPGIARNMAFASIGYIPEDGWYAGTEARYMGDIMA
                     DDENTAKAPSYTLVGLFTGYKYNYHNLTVDLFGRVDNLFDKEYVGSVIVNESNGRYYE
                     PAPGRNYGVGINIAWRFE"
     gene            complement(5604..5759)
                     /locus_tag="J1Y83_002685"
                     /pseudo
     CDS             complement(5604..5759)
                     /locus_tag="J1Y83_002685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310081.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
     gene            complement(5759..5827)
                     /locus_tag="J1Y83_002686"
                     /pseudo
     CDS             complement(5759..5827)
                     /locus_tag="J1Y83_002686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415966.6"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase yncb"
     gene            6008..6526
                     /gene="mddA"
                     /locus_tag="J1Y83_002687"
     CDS             6008..6526
                     /gene="mddA"
                     /locus_tag="J1Y83_002687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine sulfoximine/L-methionine sulfone
                     acetyltransferase"
                     /protein_id="HBA7630824.1"
                     /translation="MSIRFARKADCAAIAEIYNHAVLYTAAIWNDQTVDADNRIAWFE
                     ARTIAGYPVLVSEEDGVVTGYASFGDWRSFDGFRHTVEHSVYVHPDHQGKGLGRKLLS
                     RLIDEARDCGKHVMVAGIESQNQASLHLHQSLGFVVTAQMPQVGTKFGRWLDLTFMQL
                     QLDERTEPDAIG"
     gene            6523..6972
                     /locus_tag="J1Y83_002688"
     CDS             6523..6972
                     /locus_tag="J1Y83_002688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HBA7630825.1"
                     /translation="MNQSLTLAFLIAAGIGLVVQNTLMVRITQTSSTILIAMLLNSLV
                     GIVLFVSILWFKQGMAGFGELVSSVRWWTLIPGLLGSFFVFASISGYQNVGAATTIAV
                     LVASQLIGGLVLDIFRSHGVPLRALIGPICGAILLVVGAWLVARRSF"
     gene            complement(6973..7206)
                     /locus_tag="J1Y83_002689"
     CDS             complement(6973..7206)
                     /locus_tag="J1Y83_002689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcY family protein"
                     /protein_id="HBA7630826.1"
                     /translation="MSHLDEVIARVDAAIEESVIAHMNELLIALSDDAELSREDRYTQ
                     QQRLRTAIAHHGRKHKEDMEARHEQLTKGGTIL"
     gene            complement(7292..7465)
                     /locus_tag="J1Y83_002690"
     CDS             complement(7292..7465)
                     /locus_tag="J1Y83_002690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GhoT/OrtT family toxin"
                     /protein_id="HBA7630827.1"
                     /translation="MSLYQHMLVFYAVMAAIAFLITWFLSHDKKRIRFLSAFLVGATW
                     PMSFPVALLFSLF"
     gene            7660..7755
                     /gene="yncL"
                     /locus_tag="J1Y83_002691"
     CDS             7660..7755
                     /gene="yncL"
                     /locus_tag="J1Y83_002691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response membrane protein YncL"
                     /protein_id="HBA7630828.1"
                     /translation="MNVSSRTVVLINVFAAVGLFTLISMRFGWFI"
     gene            complement(7852..9276)
                     /gene="patD"
                     /locus_tag="J1Y83_002692"
     CDS             complement(7852..9276)
                     /gene="patD"
                     /locus_tag="J1Y83_002692"
                     /EC_number="1.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminobutyraldehyde dehydrogenase"
                     /protein_id="HBA7630829.1"
                     /translation="MQHKLLINGELVSGEGEKQPVYNPATGDVLLEIAEASAEQVDAA
                     VRAADAAFAEWGQTTPKARAECLLKLADVIEENGQVFAELESRNCGKPLHSAFNDEIP
                     AIVDVFRFFAGAARCMNGLAAGEYLEGHTSMIRRDPLGVVASIAPWNYPLMMAAWKLA
                     PALAAGNCVVLKPSEITPLTALKLAELAKDIFPAGVINVLFGRGKTVGDPLTGHPKVR
                     MVSLTGSIATGEHIISHTAPSIKRTHMELGGKAPVIVFDDADIEAVVEGVRTFGYYNA
                     GQDCTAACRIYAQKGIYDTLVEKLGAAVATLKSGAPDDESTELGPLSSLAHLERVSKA
                     VEEAKATGHIKVITGGEKRKGNGYYYAPTLLAGALQDDAIVQKEVFGPVVSVTLFDNE
                     EQVVNWANDSQYGLASSVWTKDVGRAHRVSARLQYGCTWVNTHFMLVSEMPHGGQKLS
                     GYGKDMSLYGLEDYTVVRHVMVKH"
     gene            complement(9298..10092)
                     /locus_tag="J1Y83_002693"
     CDS             complement(9298..10092)
                     /locus_tag="J1Y83_002693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBA7630830.1"
                     /translation="MHSERAPFFLKLAAWGGVVFLHFPILIIAAYAFNTEDAAFSFPP
                     QGLTLRWFSVAAQRSDILDAVTLSLKVAALATLIALVLGTLAAAALWRRDFFGKNAIS
                     LLLLLPIALPGIVTGLALLTAFKTINLEPGFFTIVVGHATFCVVVVFNNVIARFRRTS
                     WSLVEASMDLGANGWQTFRYVVLPNLSSALLAGGMLAFALSFDEIIVTTFTAGHERTL
                     PLWLLNQLGRPRDVPVTNVVALLVMLVTTLPILGAWWLTREGDNGQ"
     gene            complement(10082..11023)
                     /locus_tag="J1Y83_002694"
     CDS             complement(10082..11023)
                     /locus_tag="J1Y83_002694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBA7630831.1"
                     /translation="MAMNVLQSPSRPGLGKVSGFFWRNPGLGLFLLLLGPLMWFGIVY
                     FGSLLTLLWQGFYTFDDFTMSVTPELTLANIRALFNPANYDIIVRTLTMAVAVTIASA
                     ILAFPMAWYMARYTSGKMKAFFYIAVMLPMWASYIVKAYAWTLLLAKDGVAQWFLQHL
                     GLEPLLTAFLTLPAVGGNTLSTSGLGRFLVFLYIWLPFMILPVQAALERLPPSLLQAS
                     ADLGARPRQTFRYVVLPLAIPGIAAGSIFTFSLTLGDFIVPQLVGPAGYFIGNMVYSQ
                     QGAIGNMPMAAAFTLVPIVLIALYLAFVKRLGAFDAL"
     gene            complement(11024..12037)
                     /locus_tag="J1Y83_002695"
     CDS             complement(11024..12037)
                     /locus_tag="J1Y83_002695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBA7630832.1"
                     /translation="MTYAVEFDNVSRLYGDVRAVDGVSIAIKDGEFFSMLGPSGSGKT
                     TCLRLIAGFEQLSGGAISIFGTPASNLPPWERDVNTVFQDYALFPHMSILDNVAYGLM
                     VKGVNKKQRHAMAQEALEKVALGFVQQRKPSQLSGGQRQRVAIARALVNEPRVLLLDE
                     PLGALDLKLREQMQLELKKLQQSLGITFIFVTHDQGEALSMSDRVAVFNNGRIEQVDS
                     PRDLYMRPRTPFVAGFVGTSNVFDGLMAEKLCGMTGSFALRPEHIRLNTPGEMQANGT
                     IQAVQYQGAATRFELTLSGGEKLLVSQANMTGEELPATLTPGQQVMVSWSRDVMVALV
                     EER"
     gene            complement(12055..13200)
                     /locus_tag="J1Y83_002696"
     CDS             complement(12055..13200)
                     /locus_tag="J1Y83_002696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBA7630833.1"
                     /translation="MSKTFARSSLCALSMAIMTAHATEPPTNLENPEGRLDIIAWPGY
                     IERGQTDKQYDWVTQFEKETGCAVNVKTAATSDEMVSLMAKGGYDLVTASGDASLRLI
                     MGKRVQPINTALISNWKALDPRVVKGDWFNVGGKVYGTPYQWGPNLLMYNTKTFPTPP
                     DSWQVVFVEQNLPDGKSNKGRVQAYDGPIYIADAALFVKATQPQLGISDPYQLTEEQY
                     QAVLKVLRDQHSLIHRYWHDTTVQMSDFKNEGVVASSAWPYQANALKAEGQPVATVFP
                     KEGVTGWADTTMLHSEAKHPVCAYKWMNWSLTPKVQGDVAAWFGSLPVVPQGCKASPL
                     LGEKGCETNGFNYFDKIAFWKTPIAEGGKFVPYSRWTQDYIAIMGGR"
     gene            complement(13445..14851)
                     /locus_tag="J1Y83_002697"
     CDS             complement(13445..14851)
                     /locus_tag="J1Y83_002697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HBA7630834.1"
                     /translation="MKKYQQLAEQLREQIASGIWQPGDRLPSLRDQVALSGMSFMTVS
                     HAYQLLESQGYIIARPQSGYYVAPQAIKMPKATVIPVTRDEAVDINTYIFDMLQASRD
                     PSVVPFASAFPDPRLFPLQQLNRSLAQVSKTATAMSVIENLPPGNVELRQAIARRYAL
                     QGITISPDEIVITAGALEALNLSLQAVTEPGDWVIVENPCFYGALQALERLRLKALSV
                     ATDVKEGIDLQALELALQEYPVKACWLMTNSQNPLGFTLTPQKKVRLVALLNQYNVTL
                     IEDDVYSELYFGREKPLPAKAWDRHDGVLHCSSFSKCLVPGFRIGWVAAGKHARKIQR
                     LQLMSTLSTSSPMQLALVDYLSTRRYDAHLRRLRRQLAERKQRAWQALLRYLPAEVKI
                     HHNDSGYFLWLELPEPLDAGELSLVALTHHISIAPGKMFSTGENWSRFFRFNTAWQWG
                     EREEQAVKQLGKLIQERL"
     gene            complement(14930..15166)
                     /gene="hicB"
                     /locus_tag="J1Y83_002698"
                     /pseudo
     CDS             complement(14930..15166)
                     /gene="hicB"
                     /locus_tag="J1Y83_002698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415955.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin HicB"
     gene            15345..15575
                     /locus_tag="J1Y83_002699"
     CDS             15345..15575
                     /locus_tag="J1Y83_002699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2554 family protein"
                     /protein_id="HBA7630835.1"
                     /translation="MFSKALSVVLLTCALFSGQLMAGHKGHEFMWVKNVDHQLRHEAD
                     SDELRAVAEESAEGLREHFYWQKSRKPEAGQR"
     gene            complement(15667..17628)
                     /gene="rlhA"
                     /locus_tag="J1Y83_002700"
     CDS             complement(15667..17628)
                     /gene="rlhA"
                     /locus_tag="J1Y83_002700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA 5-hydroxycytidine C2501 synthase"
                     /protein_id="HBA7630836.1"
                     /translation="MTVSSHRLELLSPARDAAIAREAILHGADAVYIGGPGFGARHNA
                     SNSLKDIAELVPFAHRYGAKIFVTLNTILHDDELEPAQRLITDLYQTGVDALIVQDMG
                     ILELDIPPIELHASTQCDIRTVEKAKFLSDVGFTQIVLARELNLEQIRAIHQATDATI
                     EFFIHGALCVAYSGQCYISHAQTGRSANRGDCSQACRLPYTLKDDQGRVVSYEKHLLS
                     MKDNDQTANLGALIDAGVRSFKIEGRYKDMSYVKNITAHYRQMLDAIIEERGDLARAS
                     SGRTEHFFVPSTEKTFHRGSTDYFVNARKGDIGAFDSPKFIGLPVGEVLKVAKDHIDV
                     AVTEPLANGDGLNVMIKREVVGFRANTVEKTGENQYRVWPNEMPADLHKIRPHHPLNR
                     NLDHNWQQALTKTSSERRVAVDIELGGWQEQLILTLTSEEGVSITHTLDGQFDEANNA
                     EKALNNLKDGLAKLGQTIYYARNVQINLPGALFVPNSLLNQFRREAADMLDAARLASY
                     QRGSRKPVADPAPVYPQTHLSFLANVYNQKAREFYHRYGVQLIDAAYEAHEEKGEVPV
                     MITKHCLRFAFNLCPKQAKGNIKSWKATPMQLVNGDEVLTLKFDCRPCEMHVIGKIKN
                     HILKMPLPGSVVASVSPDDLLKTLPKRKG"
     gene            complement(17701..18237)
                     /locus_tag="J1Y83_002701"
     CDS             complement(17701..18237)
                     /locus_tag="J1Y83_002701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBA7630837.1"
                     /translation="MENLARFLSTTLKQLRQQRGWSLSRLAEATGVSKAMLGQIERNE
                     SSPTVATLWKIATGLNVPFSTFISPPQSSTPSVYDPQRQAMVITSLFPYDPQLCFEHF
                     SIQMAPGAISESTPHEKGVIEHVFVIDGQLDLCVDGEWQSLNCGEGVRFAADVTHVYR
                     NGGEQTVHFHSLIHYPRS"
     gene            18323..19501
                     /gene="ydcO"
                     /locus_tag="J1Y83_002702"
     CDS             18323..19501
                     /gene="ydcO"
                     /locus_tag="J1Y83_002702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001748359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BenE family transporter YdcO"
                     /protein_id="HBA7630838.1"
                     /translation="MTMRLFSIPPPTLLAGFLAVLIGYASSAAIIWQAAIVAGATTAQ
                     ISGWMTALGLAMGVSTLTLTLWYRVPVLTAWSTPGAALLVTGLQGLTLNEAIGVFIVT
                     NVLIVLCGITGLFARLMRIIPHSLAAAMLAGILLRFGLQAFASLDGQFTLCGSMLLVW
                     LATRAVAPRYAVIAAMIIGIVIVIAQGDVVTTDVVFKPVLPTYIPPDFSFAHSLSVAL
                     PLFLVTMASQNAPGIAAMKAAGYSAPVSPLIVFTGLLALVFSPFGVYSVGIAAITAAI
                     CQSPEAHPDKDQRWLAAAVAGIFYLIAGLFGSAITGMMAALPVSWIQMLAGLALLSTI
                     GGSLYQALHNERERDAAVVAFLVTASGLTLFGIGSAFWGLIAGGVCYVVLNLIANRNR
                     "
     gene            complement(19536..20204)
                     /locus_tag="J1Y83_002703"
     CDS             complement(19536..20204)
                     /locus_tag="J1Y83_002703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3313 domain-containing protein"
                     /protein_id="HBA7630839.1"
                     /translation="MRTTSFAKVAALCGLLALSGCASKITQPDKYSGFLNNYSDLKET
                     TSATGKPVLRWVDPSFDQSKYDSIVWNPITYYPVPKPSTQVGQKVLDKILNYTNTEMK
                     EAIAQRKPLVTTAGPRSLIFRGAITGVDTSKEGLQFYEVVPVALVVAGTQMATGHRTM
                     DTRLYFEGELIDAATNKPVIKVVRQGEGKDLNNESTPMAFENIKQVIDDMATDATMFD
                     VNKK"
BASE COUNT         4848 a         5181 c         5043 g         5142 t
ORIGIN      
        1 atggaacagg actaattcca gaaactcctg ttgggcaata gcgcgtttat caccctacat
       61 gcctcttaca ttttttagcc ttttctgtaa cgtcggcaat tggctcattt aatcagcaat
      121 tgtattggta tttggcgtat tacccctgaa acccatcatg tgctagcccc tcactattca
      181 ttgagccaac cacgccagtg tccactgcaa ccactggcat tcagagcgac gcacgagtgg
      241 cgcaagagta ggactgcagt caggtatcat caaaccaata gtggttgttt ccgtcataat
      301 ttcatcaggt tgctattttg tcgccagccg cctttatccc cacatattta tagcgggatt
      361 attttgcatc gttacatgat aaaaggtagg attctgcgcc gacagttata cccataaaga
      421 ataatggtga atcttgtcat cgccaggatg aataaacatt gttcatggca acttatatga
      481 ctttttcatt aaagcaatca gggagagcaa tgagtaaaca caacaccgac acttcagatc
      541 aacacgccgc gaaacgccgc tggcttaatg cccacgaaga ggggtatcac aaagcgatgg
      601 gcaatcgcca ggtacagatg atcgccattg gcggcgcgat tggtacgggt ttgtttttag
      661 gcgcgggggc gcgactgcaa atggcggggc ccgcactggc actggtctat ttgatctgcg
      721 gcctgttttc gttttttatt ctgcgtgcat tgggtgagct ggtgttacac cgcccttcca
      781 gtggcagttt tgtctcttat gcccgtgagt ttttgggtga gaaggccgct tatgttgctg
      841 gctggatgta cttcatcaac tgggcgatga cggggattgt tgatattacc gccgtcgctc
      901 tgtatatgca ttactggggc gcgtttggcg gcgtgccgca gtgggtcttt gcgctcgctg
      961 cactgaccat cgttggcacc atgaatatga tcggtgtgaa atggtttgcg gagatggagt
     1021 tctggtttgc gcttattaaa gtgctcgcca ttgtaacctt tttggtcgtg ggtacagtgt
     1081 tcctcggtag tggtcagccg ctggatggca acaccactgg ctttcattta atcaccgata
     1141 atggcggctt tttcccccat ggtttgctgc ctgcgctggt gttgattcag ggcgtagtgt
     1201 ttgcttttgc ctccattgaa atggtgggta cagcggccgg agaatgtaaa gatccgcaga
     1261 ccatggtgcc taaagcgatt aacagtgtga tttggcgtat tggtctgttt tacgtcggct
     1321 ccgtggtgtt gctggttatg ttattgccgt ggagcgcgta tcaggcgggg caaagtccat
     1381 ttgtgacgtt tttctctaaa ctgggtgtgc cgtacatcgg cagcattatg aacattgtgg
     1441 tgctgaccgc tgccctctcc agcctgaatt caggtctgta ctgcaccggg cgtattctgc
     1501 gctcgatggc gatgggcggt tccgcaccga gttttatggc gaaaatgagt cgtcagcatg
     1561 tgccgtatgc cgggattctg gcgacactgg ttgtgtatgt cgtcggcgta ttcctcaact
     1621 atctggtgcc gtcacgcgta tttgagattg tgttgaactt cgcgtcgctg ggaatcatcg
     1681 cttcatgggc gtttatcatc gtttgccaga tgcgcctgcg taaagcaatt aaagaaggca
     1741 aagcagcgga tgtcagtttt aaactgcctg gcgcgccctt cacttcctgg ctgacattac
     1801 tgtttttact gagtgtcctg gtgctgatgg cgttcgatta cccgaacggg acttatacca
     1861 tcgcggcact gccgattatc ggtattctgc tggttatagg ctggtttggt gtgcgcaaac
     1921 gcgttgctga aattcacagc actgcgccag tcgtcgagga agatgaagaa aaacaggaaa
     1981 ttgtgtttaa gcctgaaacg tcgagttaat tgcaaaattg cccggctacg tgccgggcaa
     2041 attcagacta ctgacgaagg tgccggatgc ggcgtgataa aagtcaggca attctttccg
     2101 ttttcaccct cgccatatct attacagcgc aatacgaatc acatcgtccg gctgggtagc
     2161 ttcctgctgt ttagtggatt tttgtttcac actgacatac agcgttttgc catcggcaga
     2221 cagcgccagg ctgttcggat gagtcggcgt atcgaacgtt ttcaccactt tatagctttt
     2281 cgcgtcaatc acactgactt tacctgcctg acgatgcgtc acgtaggctt cattacgcgc
     2341 tgggttaaac agcacagcca gtgattccgg tgccgcaacc ttcgccagaa tattgccatt
     2401 acgggtatcg accactaaca cttctgccgc tttagaatcg gtgataaatg cacgctgatt
     2461 gttggtatca aggctgatgt tgataaagaa gtgctctttg ccgtcatcca gcagcttttt
     2521 acggctgagg attttattgt cggcggtgtc gatggtaatc aattcgccgt cagcgttagt
     2581 ggtgtaaaga cgtttgcctt tgctatccag cgccagaccg gtactcattt taccggtgtt
     2641 ctggatggcg gttttcagtt tgatattctc gccatcaacg acccaaatca cgctctcttt
     2701 accaatacca ctgatgtaaa cggtgttcgt ggcatcatca gctaccagct cacgtggttg
     2761 caacgggcgt acctcttccg tgcgcttacg atcatccagc accagacgac ctttcacttc
     2821 gcccgttttg gcatctatcg ccgtgaccgc actatttacg gtgttaccaa accacaacgt
     2881 ctgagtcgtg ttattgatgg tggcaccaaa cggcttgaga tcgttatgga tcgcctgcgt
     2941 cacttccaga gtaaccggat caagacgata aaccacgccg cctttatcca gtttgcggct
     3001 ttgcgaagtg gcgagccaca gcgcgttttc ttgctggcta taagccattt cgtaggcacc
     3061 tttacctacc gctttacgca gcatttcttc tgcggcctgc gtactgaatg atgaagcaac
     3121 aagcaatgaa cctaacagta atgaaccacg caggcgcgat gaaaacagat gacgtaaatg
     3181 catgacgact ccctttgatg aaccgatatt tttgctgctt cacaatgctt cgctaagcgt
     3241 aatcatcgtt acgcccttgt tataaatggg aatggtaatc attattttct caaatgtgga
     3301 gcatttctta ccgttacgac tacaattttc tcaattactt aacgtattgt gccgtttcgt
     3361 tttccataaa tttacaattt cattaccatg acggtgtttt cccattctta acctggcttt
     3421 tttcatgaag attttttccg tccgacagac cgttttgccc gcactgcttg tcctttcccc
     3481 cgttgttttt gccgctgatg aacagaccat gattgtcagt gccgcatcgc aggtggtttc
     3541 agaactggat accccagcag ccgtgagcgt ggtggatggc gaggagatgc gcctggcaac
     3601 accgcgcatt aacttgtctg aatcactgac cggagtgcct ggtttacagg tacaaaaccg
     3661 gcagaactat gcgcaagatt tacagctgtc gattcgcgga tttggctccc gctccactta
     3721 cggaattcgc ggtattcgcc tgtatgtgga cggtattccc gctaccatgc ccgacggaca
     3781 agggcaaaca tctaacatcg atttaagcag tgtgcaaaat gtggaagtgc tgcgtggccc
     3841 cttctctgct ctgtatggca acgcgtctgg cggtgtaatg aatgtcacca cccagaccgg
     3901 acaacagcca ccaaccattg aagccagtag ttactacggc agttttggca gctggcgcta
     3961 tgggctgaaa gctacgggcg caacgggcga cggcacacaa cccggcgatg tcgattacac
     4021 cgtctcaacc acacgtttta cgacccacgg ctatcgtgac catagcggcg cacagaaaaa
     4081 tttagccaat gccaaactgg gcgtacgcat tgatgacgcc agcaaattaa gcctgatttt
     4141 caatagtgtg gatatcaaag cagatgaccc aggtgggcta accgaagcag aatggaaggc
     4201 gaatccacaa caagcgcccc gtgcagaaca gtacgacacg cgaaaaacca tcaagcaaac
     4261 tcaggctggg ttgcgctatg aacgtagcct gagcgcgcaa gatgatatga gtgtaatgat
     4321 gtatgccgga gagcgagaaa cgacccagta ccagtcaatc cccatggccc cgcaacttaa
     4381 cccgtcacat gcgggcggcg tgattaccct gcaacgccat taccagggga tagacagccg
     4441 ctggacacac cgtggtgaat tgggcgttcc agtcacgttc acaaccggcc tgaactacga
     4501 aaacatgagt gaaaaccgca agggttacaa taacttccgc ctgaatcgcg gcgtgccgga
     4561 atacgggcaa aaaggtgagt tgcgccgcga cgaacgcaat ttgatgtgga acgtcgatcc
     4621 ctatttacaa acccagtggc aactgaccga caaactttcg ctggatgctg gcgtgcgcta
     4681 cagctctgtg tggtttgatt ccaatgatca ttacgttact ccgggtaacg gcgacgacag
     4741 cggtgatgcc agttatcaca aatggctacc agccggatcg ttaaaatatg caatgaccga
     4801 tgcctggaat gtctatctgg cagccgggcg cggttttgaa acgccgacga ttaatgagct
     4861 gtcttatcgc gctgatgggc aaagcggtat gaactttggt ttaaaaccct ccaccaacga
     4921 tacaattgag atcggcagta aaacgcgtat tggtgatggg ctgcttagtc tcgcattgtt
     4981 ccagaccgac accgatgatg aaattgttgt cgatagcagt agcggtgggc gtacgactta
     5041 caaaaatgcc ggaaagaccc gtcgtcaagg cgctgaactg gcatgggatc aacgtttcgc
     5101 gggagatttt cgcgtaaaag cgtcctggac ctggcttgat gcgacctatc gcagcaatgt
     5161 ttgcaatgaa caggattgta acggtaaccg gatgccaggg atcgcccgta atatggcctt
     5221 tgcgtcgata ggttatatac cggaagatgg ttggtatgca ggcacggaag cgcgttatat
     5281 gggcgatatt atggcagatg atgaaaatac ggccaaagcg ccttcttata cactcgtcgg
     5341 cttattcacc gggtataaat acaattacca caatttaacc gtggatttat ttggtcgtgt
     5401 cgataattta ttcgataaag aatacgttgg ttctgtcatt gtcaatgagt caaacgggcg
     5461 atattacgaa cctgcgcccg gacgaaatta tggtgtcggc ataaatattg catggcgatt
     5521 tgagtaatta tctttaagcc cggtatttgc cgggcgtatt ttatttatcg gctgtattgc
     5581 tggcacaaca tagcaacgtt tttttgcata gtgaggtttt cgacagccag agcaactgcc
     5641 attaattcca gttcgtagcg gatccgattg atttcattta attgacgttt tcccacttcc
     5701 ggaactgtaa atgcttgcgc tggtgcgaca aagagcgcat ttaccgaaac cagacgtaag
     5761 gaaccggcgc gccatgcgga cgcgaggcca gaacccaaca tcggttacgc tgcgtttgtt
     5821 gccccataat gctctcctgt gcttttggca tgaatccatc aatagtagca agctcacagg
     5881 attggttttg tttgcagccg tcacatcccc tctgcaacac gcaataattt catagaggaa
     5941 tccgttgctt cgggctgttt tcgatagtat tgataacatc atcatcatta ttttatcgag
     6001 gccagacatg tccatccgtt ttgcccgcaa agccgactgt gctgcaattg cggaaattta
     6061 taaccacgcc gtgttgtata cggcggctat ctggaatgac caaacggtgg atgcggataa
     6121 ccgcattgcc tggtttgaag cgcggaccat agcaggttat ccggtgctgg tgagtgaaga
     6181 agatggcgta gtgacgggat atgcctcgtt tggcgactgg cgtagttttg atggttttcg
     6241 ccataccgtg gaacattcgg tttatgtcca tcccgatcat caggggaaag gtctggggcg
     6301 taaattgtta agccgattga ttgatgaagc gcgggattgc gggaagcatg tcatggtcgc
     6361 cgggatcgaa tcgcaaaatc aggcctcgct gcatctgcac cagtcgctgg gatttgtcgt
     6421 caccgcgcaa atgccacagg taggtactaa atttgggcgc tggctggatc tgacatttat
     6481 gcagttgcaa ctcgacgagc gcactgaacc ggacgcgatt ggatgaatca gtcacttacc
     6541 cttgcctttt tgattgcagc cgggattggt ctggtagtcc agaacacgct gatggtgcgc
     6601 atcacccaga cctcctctac cattctcatc gccatgttgc tgaactcact ggtggggatt
     6661 gtgctctttg tttctatttt atggtttaaa caaggcatgg cggggtttgg cgaactggtg
     6721 tccagcgtgc gctggtggac acttatacct ggcttgctgg gatcgttttt tgtcttcgcc
     6781 agtatcagcg gatatcagaa tgtgggagcg gcgacaacta ttgcagtact ggtcgccagc
     6841 cagctgattg gcggtctggt gctggatatc ttccgcagcc atggcgtacc acttcgggcg
     6901 ttgatcggcc caatttgtgg cgcaatttta cttgtggttg gcgcctggct ggtggccaga
     6961 cgctcatttt aatcagagga tagtaccgcc tttggttaac tgttcgtggc gcgcttccat
     7021 atcttcttta tgtttgcgac cgtgatgagc aatagcggtg cgcaaacgct gctgctgggt
     7081 gtagcgatct tcccgactta actccgcgtc atcgctcagg gcgattaata attcgttcat
     7141 atgggcaatg acgctctctt caatcgcggc atccacgcgc gcgatgactt catccagatg
     7201 cgacatactt tctccttagc cacttgtgaa atttatcgat tcatccaaat gatataagtc
     7261 tgtgtggtgc cgtcaagcca gtgagtaaga tttaaaacag tgaaaacaac agcgccaccg
     7321 ggaaactcat cggccatgtt gcccccacca gaaaggcgct taagaaacgg atgcgtttct
     7381 tatcgtgaga aagaaaccag gtgataagaa atgcgattgc tgccataacc gcataaaaaa
     7441 caagcatgtg ttgatagaga gacatgtacg ggcgtccagc cagatgaata aaacgcgcgc
     7501 aaatatgcgt cattttttga cgtgcatcaa gttttttcac cagtttgaga aaaaatcatc
     7561 aaacagcaac ttgcccttat ccatcttcag aatgagtatt attgcggatt ttcttaaccc
     7621 gtactataca cataggggca atgataaaag gtggcaaaaa tgaatgtttc cagtagaact
     7681 gtagtactga taaatgtctt tgctgctgtt ggtttgttta ctcttatctc tatgagattt
     7741 ggctggttta tttgatgtag tgattttgca cgcagcacgt actgtcagag aaatcagtgc
     7801 tctgacagta cgcaagatca tctcatactc ttacgatctg caatattatc cttaatgttt
     7861 aaccatgacg tggcggacga cggtgtaatc ctccagccca taaagtgaca tatccttgcc
     7921 gtaaccagaa agtttctgcc caccgtgcgg catttcactt accagcatga aatgggtatt
     7981 gacccaggta caaccatatt gcagccgcgc gctgacgcga tgcgccctgc ccacatcttt
     8041 cgtccatacc gaagatgcaa gtccgtactg actgtcattc gcccagttca ccacctgttc
     8101 ttcgttgtcg aagagcgtaa cactcactac cggaccaaat acctcttttt gcacgatggc
     8161 atcgtcctgt aatgcgccag ccagcagcgt cggcgcatag taatagccat tacccttgcg
     8221 cttttcaccg ccagtgatca ctttgatgtg ccctgtcgct ttcgcctctt ctaccgcctt
     8281 gctgacgcgt tcgagatgcg ccagcgagct taaaggtcca agctccgtag actcgtcatc
     8341 cggtgcacca gattttaacg ttgccaccgc agcaccgagt ttttccacca gcgtatcgta
     8401 aatgcctttt tgcgcgtaga tccgacaagc cgcagtacaa tcctgtccgg cattgtaata
     8461 gccaaatgta cgtacacctt cgaccactgc ttcaatatcc gcatcatcaa aaacaatcac
     8521 tggcgctttg ccaccaagtt ccatatgagt acgcttaatg gacggcgcgg tatggctgat
     8581 gatgtgctcg ccggtggcga tagagcccgt cagcgacacc atccgcactt tgggatgtcc
     8641 ggtcagcgga tcgcccaccg ttttgcctct gccaaacagt acgttaatca cgcctgccgg
     8701 gaagatatct ttcgccagct ctgccagctt caacgcggtc agcggggtaa tttctgatgg
     8761 tttgagcact acgcagttcc ctgccgccag cgccggagcc agtttccacg ctgccatcat
     8821 cagcggataa ttccacggtg cgatagaagc cacgaccccc aacggatcgc gacggatcat
     8881 cgaagtatga ccttcaagat attcacctgc cgccagacca ttcatacagc gcgccgcacc
     8941 agcgaaaaag cgaaaaacat cgacaatcgc tgggatttca tcattgaatg cactatgcag
     9001 cggtttgcca caattacggg attccagttc ggcaaaaacc tgaccatttt cttcgataac
     9061 atcagccagt ttcagcaggc attccgcacg cgcttttggc gtggtttgcc cccattcggc
     9121 aaatgctgca tctgccgcgc gaacagcagc atcgacctgc tctgcggatg cctcggcaat
     9181 ctccagtaaa acgtcccccg ttgccggatt atagacaggc tgtttttccc cttctccgct
     9241 aaccagttct ccgttaatca gtaacttatg ttgcatagca tattcctgta tcagtggtta
     9301 ttgaccattg tcgccttcgc gggttagcca ccaggccccc aggatcggca aggttgttac
     9361 caacataacc agcagtgcca ccacgttggt taccggtaca tcacgcggtc gcccaagctg
     9421 attgagcaac cacaacggta acgttcgttc atgacctgcc gtaaaagtcg tcacgatgat
     9481 ttcatcgaac gacaaggcaa acgccagcat tcctccagcc agtaacgccg aactgagatt
     9541 cggcaacact acgtagcgga aggtttgcca gccattggcc ccaagatcca ttgacgcctc
     9601 aaccagactc cacgaggtgc ggcgaaaacg ggcgatgaca ttgttaaaca ccaccactac
     9661 gcaaaaagtc gcatgaccga ccacgatggt gaaaaatccc ggctccagat tgatggtttt
     9721 aaaagcggtt aatagcgcca gaccagtgac aatgcccggc agcgcaatgg gcagcaacag
     9781 taacagcgaa atggcgtttt tgccgaaaaa gtctcgtcgc cacagcgcgg cagctgccag
     9841 cgtccctaac accagcgcga ttaatgtcgc cagcgccgcc actttaagtg acagtgtcac
     9901 ggcatcaaga atatcactac gctgtgctgc cacgctaaac cagcgcagcg tcaggccttg
     9961 cggtggaaaa ctaaacgccg catcttcagt gttaaacgca taggcagcga taatcaggat
    10021 gggaaaatgt aggaaaacca cgccgcccca ggccgccagt ttgaggaaaa acggtgcgcg
    10081 ttcagagtgc atcgaacgct cccagacgtt tcacgaacgc caggtaaagt gcgatgagaa
    10141 caatcggcac cagggtgaat gccgccgcca tcggcatatt gccaatcgcc ccctgctggg
    10201 aataaaccat attgccgata aaatatcctg ccgggccaac cagctgcggg acgataaaat
    10261 cgcccagtgt gagtgagaag gtaaagatag agccagcggc aatacccggg attgccagcg
    10321 gcagcaccac atagcgaaag gtttgtcgtg gacgtgcgcc aagatcagcc gacgcctgca
    10381 acaatgacgg cggcaaacgc tcaagcgccg cctgcaccgg gaggatcatg aacggcaacc
    10441 agatatagag aaacaccaga aagcgcccca gcccggaagt tgacagcgta ttacctccca
    10501 ccgccggtag cgtgaggaac gcagtcagca gtggttccag ccccagatgt tgtaaaaacc
    10561 actgcgccac gccatctttt gccagcaata acgtccaggc ataggcttta acaatgtagc
    10621 tcgcccacat cggcaacatt accgcgatat aaaaaaacgc tttcattttt ccgctggtat
    10681 agcgcgccat ataccacgcc attggaaaag ccagaatggc gctggcgata gtgactgcca
    10741 cagccatggt cagcgtgcgg acaatgatgt cgtaattcgc cggattaaac agcgcacgga
    10801 tattcgccag cgtcagttcc ggcgttaccg acatggtgaa atcgtcaaag gtataaaagc
    10861 cctgccataa cagcgtcagc agcgagccga aatagacaat gccaaaccac attagcgggc
    10921 caagcagcag caaaaacagc cccagccccg gattacgcca gaagaaaccg gacaccttac
    10981 ccagacctgg acgtgaaggt gattgcaata cgttcatcgc cattcacctc tcctcaacca
    11041 gcgccaccat cacatcacgc gaccaggaaa ccatcacctg ttgtccgggc gtgagcgtgg
    11101 caggcagttc ttcgcctgtc atattggcct gactcaccag cagtttttca ccgccgctca
    11161 gtgtcagttc aaaacgggtc gctgcgccct gatattgcac cgcctggatc gtgccattag
    11221 cctgcatttc gccaggggtg ttgaggcgga tatgttccgg tcgtagtgcg aagcttcccg
    11281 tcatgccaca aagtttctct gccatcagtc catcaaaaac attcgatgtc ccaacgaatc
    11341 ccgcaacaaa cggcgtgcgc gggcgcatat agagatcgcg cggagaatcg acctgttcaa
    11401 tgcgtccatt attgaaaacc gccacccgat cggacatcga taacgcttcg ccctgatcgt
    11461 gggtaacgaa gataaaagtg ataccgagag actgttgcag ctttttcagc tcaagctgca
    11521 tttgctctcg caatttgaga tccagcgcgc cgagcggttc atccagcaac aacacacgcg
    11581 gttcgttcac cagtgctctg gcgatggcga ctcgctggcg ctgaccacca gaaagttgtg
    11641 acggtttacg ttgctgtaca aaccccaacg ccactttctc aagcgcctct tgcgccattg
    11701 catgccgctg ctttttattc acgcctttga ccatcagccc gtaggctacg ttgtcaagaa
    11761 tcgacatatg cggaaatagc gcgtagtcct ggaagacagt attcacgtcc cgctcccacg
    11821 gtggcagatt gctggcgggc gtaccaaaaa tagagatagc cccgccggaa agctgttcga
    11881 atccagcaat caggcgcagg caagtggttt tgccggagcc ggacggcccc agcatagaga
    11941 agaactcacc atcttttatc gcaatactga cgccatctac tgcgcgcacg tcaccgtaca
    12001 accgtgagac gttgtcaaac tccactgcgt acgtcataaa acaccccagc gaagttagcg
    12061 accgcccata atggcaatgt aatcctgcgt ccagcgactg tagggaacaa acttgccccc
    12121 ttctgctata ggcgttttcc agaaggcgat tttatcgaaa tagttaaaac cgtttgtttc
    12181 acaacctttc tcgcctaata acggactggc tttacaccct tgcggcacta ctggtagcga
    12241 gccaaaccag gccgccacat cgccctgcac ttttggggtt aatgaccagt tcatccattt
    12301 gtaggcgcaa accggatgtt tcgcttcgct atgtagcatg gtggtgtcag cccaaccggt
    12361 aacgccctcc ttcgggaaaa cggtagcgac aggctggcct tcggctttca gggcgttggc
    12421 ctgatagggc catgcactgg aagcaaccac accctcattt ttgaaatcgc tcatttgcac
    12481 ggtagtgtca tgccagtagc gatggatcaa actatgttga tcgcgcagca ctttcagcac
    12541 cgcctgatac tgttcttcgg tgagttgata cggatcgctg atgcccaact gcggctgcgt
    12601 ggctttgacg aacaacgcgg catccgcaat gtagataggg ccatcataag cctgtacccg
    12661 gcctttattg ctcttgccgt ccggcagatt ctgctcaaca aaaaccactt gccagctatc
    12721 cggcggcgtc gggaaggttt tagtgttgta catcagcagg ttcggccccc attggtaagg
    12781 tgtgccgtaa actttgccgc caacgttaaa ccagtcgcct ttaaccacgc gcggatcgag
    12841 cgctttccag ttggaaatca aggcggtatt aatcggctgc acgcgtttgc ccataatcaa
    12901 acgcagcgag gcatcgccgg atgccgtaac cagatcgtaa ccccctttgg ccatcagact
    12961 gaccatttca tcggaagtcg cggcggtttt aacattcacc gcgcagcctg tctctttttc
    13021 gaattgcgtt acccagtcgt actgtttatc agtttgtccg cgttcgatgt atcccggcca
    13081 ggcgataata tccagtcgcc cttccggatt ctctaaattg gtaggcggtt cggtggcgtg
    13141 agcggtcatt attgccatgc tgagcgcaca caggctgctg cgggcaaatg tcttgctcat
    13201 aaggtcttac tcctgtcgta attaattgtt cagccgccgt gccgtaaaaa tatctccttg
    13261 tttaaccata gacggcggct tgcaggcaca ccttcggccc tgagaaaatt taatcaggtt
    13321 gtgagctacc gcgcattatg ccgtctgtaa ggttatcttc tggagtatag acaaggagtt
    13381 aggatatatg actgctattc gaaattcata ttacccaacc tgagaggaat taaatcaaac
    13441 gctattacag ccgttcttga ataagtttgc ctaattgttt taccgcctgt tcttcacgtt
    13501 ctccccactg ccacgcggta ttaaaacgaa aaaaacgtga ccagttttca ccggtagaaa
    13561 acattttacc cggcgcaata ctgatatgat gcgtcagtgc caccaggctt aattctcctg
    13621 catctaacgg ctcggggagc tccagccaga gaaaataacc gctgtcattg tgatgaattt
    13681 tgacttccgc aggcagataa cgcagcaaag cctgccaggc gcgttgttta cgttccgcaa
    13741 gctggcgacg caggcgacga agatgagcat cgtatcggcg agtagaaagg taatccacca
    13801 gcgcaagctg catcggtgag ctggtggaaa gcgtactcat caactgcaag cgttgaattt
    13861 tacgtgcatg ttttccggcg gcgacccaac caatacgaaa accaggcacc agacatttcg
    13921 aaaacgaaga gcaatgcaaa acaccatcgt ggcgatccca cgctttcgca ggcagcggtt
    13981 tttcccgtcc aaaataaagt tcgctataaa cgtcatcttc aatcagcgtt acgttgtact
    14041 gattgagcaa cgctaccagc cgcacttttt tctgcggcgt taaggtaaat ccaagtgggt
    14101 tctggctatt agtcatcagc cagcacgctt tcaccggata ctcctgcaac gccagttcca
    14161 gcgcctgaag atcgatccct tctttaacat ccgtcgccac cgataacgcc ttcagccgta
    14221 accgctccag cgcctgcaac gcgccgtaga aacaaggatt ctctactatc acccaatcgc
    14281 ccggttcagt taccgcttgc aaactgaggt ttaatgcctc taacgccccg gcggtaatga
    14341 caatttcatc aggagaaatg gtgatgccct gtaaggcata gcgacgagca atagcctgac
    14401 gcagttctac gtttcctggc ggtaagtttt caatcacgct catcgccgtg gcggttttgc
    14461 ttacctgcgc cagcgagcgg tttagttgtt ggagggggaa aagtcgcggg tcgggaaagg
    14521 ccgaggcaaa cggaactacc gacggatcac gactggcctg caacatatca aaaatatagg
    14581 tgttgatatc gactgcttca tctcgtgtga ctggaatgac tgtcgctttc ggcattttta
    14641 ttgcctgtgg cgcaacgtaa taacccgatt gcggtcgcgc gataatatat ccctgacttt
    14701 cgagcaactg ataggcatgg ctgacagtca taaagctcat gccagaaagc gccacctggt
    14761 cacgcaacga aggcaaacga tcgccgggtt gccagatacc cgacgcaatc tgctcgcgta
    14821 attgttctgc aagctgctgg tattttttca ttatcgaatc gtaattatgt gcagatgatt
    14881 cggcagtcta tatcagttgt taaaaatgac aacttttcat taactgtaat taaaccatca
    14941 ccagcgataa ctctttgcca agcgcattag ccgcgagctg gacggcgtcg atttttgtcg
    15001 catggtgcaa attaaatagg cgagtaatct cctgtttatg tttgccaatt cgcctggcta
    15061 actcttgctg agtgatttct gactgtaaaa aagcatttaa cagcaatacc ttagaggcga
    15121 cgctcaaagg cacctcaata aagtgatcgt gactatttaa tggcgaagtt tacctccgtg
    15181 tgtaatgtgc tcgaaaatag cactaaatcg ccgcaacggc agaatgcaat gcaaaatcag
    15241 gaaggaagat tccagaattt ctttgctcta atttgcaaaa ctgaataata acctcaagaa
    15301 acgatcggca tcatagcggc aaaaggttag aaaaaggtgg cgggatgttt tcgaaggcgt
    15361 tatcggttgt cttattaacg tgtgcactgt tctcaggaca actcatggca gggcacaaag
    15421 gacatgaatt tatgtgggta aagaatgtgg atcatcagct gcgtcatgaa gcggacagcg
    15481 atgaattgcg tgctgtggcg gaagagtcgg cggaaggttt gcgcgagcat ttttactggc
    15541 aaaaatcgcg caaaccagaa gcgggacagc gttgagtcat ttgttgccgg atgcggcatg
    15601 acgccttacc cggcaggcaa tatcatgcag atttgatgag tggtgagtaa cccgaaaact
    15661 ggcgttttac ccttttcgct tcggcaatgt tttcagcaga tcatccggac ttacggatgc
    15721 cactacgctt cccggtaacg gcattttcag tatgtgattt ttgattttgc caatgacgtg
    15781 catctcgcat gggcggcaat caaactttag cgttaatact tcatcgccgt taaccagttg
    15841 cattggcgtc gccttccagc ttttgatatt gcctttcgcc tgtttcgggc acagattgaa
    15901 ggcaaagcgc agacaatgct tggtgatcat caccgggact tcgcccttct cttcatgtgc
    15961 ctcatacgcc gcgtcaatca gttgcacacc atagcgatga taaaattcac gcgctttctg
    16021 gttgtatacg ttcgcgagga aactcagatg cgtttgcgga taaactggcg caggatcagc
    16081 aaccggttta cggctgccgc gctggtaact ggcaagacgc gcagcatcaa gcatatcggc
    16141 ggcttcacgg cggaactggt ttaacagact gtttggtaca aacagcgccc ccggcaaatt
    16201 aatttgcaca ttgcgggcgt aatagatggt ttgccccagc ttagccagac catctttcag
    16261 gttattaagc gctttttcgg cgttattggc ttcgtcgaac tgcccgtcca gcgtatgtgt
    16321 gatgctgaca ccctcttcac tggtgagggt cagaatcagt tgttcctgcc agccgcccag
    16381 ttcaatgtct accgccaccc ggcgttcgct ggaggttttt gtcagtgcct gctgccagtt
    16441 atgatcaaga tttcggttta gtgggtgatg cgggcgaatt ttgtgcaaat ctgctggcat
    16501 ttcatttggc cagacgcggt actgattttc tccggttttc tctaccgtat tggcacgaaa
    16561 accgacgact tcacgtttaa tcatcacgtt caggccatcg ccatttgcca gtggctcggt
    16621 aacggcaaca tcaatatgat ctttcgctac tttcaatact tcgcccaccg gcaggccgat
    16681 aaatttcggc gaatcgaacg cgccaatatc gcctttacgg gcattcacaa aataatctgt
    16741 gctaccacgg tggaaagtct tttccgtcga tggaacaaag aaatgttcag tacgacctga
    16801 tgaagcgcgc gccagatcgc cacgttcttc aataatggca tccagcatct ggcgataatg
    16861 ggcggtgata ttcttcacgt agctcatatc tttgtaacgc ccttcaatct tgaaggagcg
    16921 tacgccagca tcaatcagcg cgccgaggtt ggcagtctga tcgttatctt tcatcgacag
    16981 cagatgtttt tcataggaaa ccacccgccc ctgatcgtct ttcaatgtgt atggcaaacg
    17041 gcacgcctgc gagcaatcgc cacggttggc gctacgccct gtttgcgcat gagaaatgta
    17101 gcactgaccc gaataggcca cgcacaatgc gccatgaata aagaattcaa tggtcgcgtc
    17161 cgtagcctgg tgaatcgcgc ggatctgctc aagattcagc tctcgcgcca gcacaatctg
    17221 cgtgaagcca acatcagaga ggaacttcgc tttttctact gtacgaatat cgcactgcgt
    17281 actggcgtgc agttcaatcg gcggaatatc aagttccaga atccccatat cctgaacaat
    17341 cagcgcatcg acaccggtct ggtagaggtc agtaatcagc cgttgcgcgg gttccagctc
    17401 atcatcatgc aaaatggtgt taagcgtgac gaaaattttt gcaccataac gatgggcaaa
    17461 cggcaccaac tcggcaatat ctttcaggct attactggca ttatgacggg caccaaaacc
    17521 agggccgccg atataaacag catcggcacc gtgcaaaata gcttcgcggg caatggcggc
    17581 atcgcgtgcc gggcttaaca gttcaagacg atgagaagat acggtcactt tcttatccag
    17641 tagctgaaaa atggcggcta ttttagccac aagcgcgacg ttgcgaaata gttttcccgc
    17701 ttaactgcgc gggtaatgga tgagggaatg aaaatgtacg gtttgctccc caccattacg
    17761 atagacatgc gtgacgtctg cagcaaatcg aacgccttcc ccacaattaa ggctctgcca
    17821 ttcaccatca acacacagat caagttgtcc atcaatgacg aagacatgtt cgattacccc
    17881 cttttcatgg ggcgtcgatt cgcttattgc accgggtgcc atctgaatcg agaagtgttc
    17941 aaagcagagt tgtggatcat aaggaaacag cgaagtgatc accatcgcct gccgttgtgg
    18001 atcataaact gaaggtgtag aggactgcgg cggagaaata aatgttgaaa atggcacgtt
    18061 caacccggtg gcaattttcc ataatgtcgc gaccgtcggg ctggattcat tacgctcaat
    18121 ctgccctaac atcgctttag aaacgcccgt cgcttctgcc agtcgcgaaa gactccagcc
    18181 gcgctgctga cgtagttgtt taagtgtggt ggataaaaag cgagcgagat tttccatgcg
    18241 actcctccgg caaaacggaa gtttatcact tgtgcgttat aacggacaaa tgctacgtta
    18301 gccgtacgtt ataacgcacg aggtgactat gcgtctgttt tctattcctc cacccacgct
    18361 actggcgggg tttctggcgg tattaattgg ctacgccagt tcagcggcaa taatctggca
    18421 agcagcgatt gtcgccggag ccaccactgc acaaatctct ggctggatga cggcgctggg
    18481 gctggcaatg ggcgtcagta cgctgactct gacattatgg tatcgcgtac ctgttctcac
    18541 cgcatggtca acgcctggcg cggctttgtt ggtcaccgga ttacagggac taacacttaa
    18601 cgaagccatc ggcgttttta ttgtcaccaa cgtgttaata gtcctctgcg gcataacggg
    18661 actctttgct cgtctgatgc gcattattcc gcactcgctt gcggcggcaa tgcttgccgg
    18721 gattttatta cgttttggtt tacaggcgtt tgccagtctg gatggtcaat ttacgttgtg
    18781 tggcagtatg ttgctggtat ggctggcaac cagggccgtt gcgccgcgct atgcggtaat
    18841 tgccgcgatg attattggga tcgtgatcgt tatcgcgcaa ggtgacgttg tcacaactga
    18901 tgttgtcttt aaacccgttc tccccactta tattccccct gatttttcgt ttgctcacag
    18961 tctgagcgtt gcactccccc tttttctggt gacgatggca tcgcaaaacg caccgggtat
    19021 cgcagcaatg aaagcagccg ggtattcggc tcctgtttcg ccgttgattg tgtttactgg
    19081 attgctggcg cttgtttttt cccctttcgg cgtttattcc gtcggtattg cggcaatcac
    19141 cgcggctatt tgccaaagcc cggaagcgca tccggataaa gatcaacgtt ggctggccgc
    19201 tgccgttgct ggtatttttt atttgatcgc aggtctgttt ggtagtgcca ttaccgggat
    19261 gatggctgcc ctgcccgtaa gttggatcca gatgctggca ggtcttgcgc tgttgagtac
    19321 cattggcggc agtttgtatc aggcgctgca taatgagcgt gagcgcgacg cggcggtggt
    19381 ggcatttctg gtaacggcaa gtggattgac gctgttcggg attggttctg cgttttgggg
    19441 attaattgcc ggaggcgttt gttacgtggt gttgaattta atcgctaaca gaaaccgata
    19501 ataaaaagcg ccatctaacg atggcgcgtc tggaactact ttttgttaac gtcaaacatg
    19561 gtggcatcgg tcgccatgtc atcaataact tgtttaatat tttcgaaagc cattggcgta
    19621 ctttcgttat tcaggtcttt accttcgccc tgacgaacga ccttgataac cggtttatta
    19681 gtcgctgcat caatcagctc accttcaaaa tacaggcgag tatccatggt acggtggcct
    19741 gtagccattt gcgtccccgc aaccactaat gcaacaggaa ccacttcata gaattgcagc
    19801 ccttctttac tggtatctac accggtaatg gccccacgga aaatcagact acgcggccca
    19861 gcggtggtaa ccagtggttt acgctgcgct atcgcttctt tcatttcggt gttggtatag
    19921 ttcaaaattt tatccagaac tttctgccct acctgggtag aaggtttcgg taccggataa
    19981 taagtgattg ggttccagac gatgctgtca tatttgcttt gatcaaaact cggatctacc
    20041 caacgtaaaa caggtttacc tgttgccgag gttgtttctt ttaaatcaga gtaattgttt
    20101 aaaaaaccag aatatttatc tggctgggtg attttagagg cacaaccaga cagagccaac
    20161 aagccgcata aagctgcaac tttcgcaaat gatgtggtac gcatgaatag tttc
//
LOCUS       DADSYM010000087        20178 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2415
            SAMN08039579-rid11723173.denovo.087, whole genome shotgun sequence.
ACCESSION   DADSYM010000087 DADSYM010000000
VERSION     DADSYM010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039579
            Sequence Read Archive: SRR6362533
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 20178)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20178)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980162.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 03:39:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,484
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 4,347
            CDSs (with protein)               :: 4,347
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 43 of 85
            Pseudo Genes (incomplete)         :: 37 of 85
            Pseudo Genes (internal stop)      :: 19 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20178
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039579-rid11723173.denovo.087"
                     /strain="LGE2415"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8302 N 95.9628 W"
                     /collection_date="2015-08-24"
     gene            165..317
                     /gene="hokA"
                     /locus_tag="J1Y83_002704"
     CDS             165..317
                     /gene="hokA"
                     /locus_tag="J1Y83_002704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001135724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokA"
                     /protein_id="HBA7630840.1"
                     /translation="MPQKYRLLSLIVICFTLLFFTWMIRDSLCELHIKQESYELAAFL
                     ACKLKE"
     gene            complement(505..717)
                     /gene="cspA"
                     /locus_tag="J1Y83_002705"
     CDS             complement(505..717)
                     /gene="cspA"
                     /locus_tag="J1Y83_002705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone/antiterminator CspA"
                     /protein_id="HBA7630841.1"
                     /translation="MSGKMTGIVKWFNADKGFGFITPDDGSKDVFVHFSAIQNDGYKS
                     LDEGQKVSFTIESGAKGPAAGNVTSL"
     gene            complement(998..1288)
                     /locus_tag="J1Y83_002706"
     CDS             complement(998..1288)
                     /locus_tag="J1Y83_002706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator"
                     /protein_id="HBA7630842.1"
                     /translation="MEYKDPMHELLSSLEQIVFKDETQKITLTHRTTSCTEIEQLRKG
                     TGLKIDDFARVLGVSVAMVKEWESRRVKPSSAELKLMRLIQANPALSKQLME"
     gene            1722..2432
                     /locus_tag="J1Y83_002707"
     CDS             1722..2432
                     /locus_tag="J1Y83_002707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3053 domain-containing protein"
                     /protein_id="HBA7630843.1"
                     /translation="MATGKSCSRWFAPLAALLMVVSLSGCFDKEGDQRKAFIDFLQNT
                     VMRSGERLPTLTADQKKQFGPFVSDYAILYGYSQQVNQAMDSGLRPVVDSVNAIRVPQ
                     DYVTQSGPLREMNGSLGVLAQQLQNAKLQADAAHSALKQSDDLKPVFDQAFTKVVTTP
                     ADALQPLIPAAQTFTQQLVMVGDYIAQQGTQVSFVANGIQFPTSQQASEYNKLIAPLP
                     AQHQAFNQAWTTAVTATQ"
     gene            complement(2482..3456)
                     /gene="ghrB"
                     /locus_tag="J1Y83_002708"
     CDS             complement(2482..3456)
                     /gene="ghrB"
                     /locus_tag="J1Y83_002708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate/hydroxypyruvate reductase GhrB"
                     /protein_id="HBA7630844.1"
                     /translation="MKPSVILYKALPDDLLQRLQEHFTVHQVANLSPQTVEQNAAIFA
                     EAEGLLGSNENVDAALLEKMPKLRATSTISVGYDNFDVDALTARKILLMHTPTVLTET
                     VADTLMALVLSTARRVVEVAERVKAGEWTASIGPDWYGTDVHHKTLGIVGMGRIGMAL
                     AQRAHFGFNMPILYNARRHHKEAEERFNARYCDLDTLLQESDFVCLILPLTDETHHLF
                     GAEQFAKMKSSAIFINAGRGPVVDENALIAALQKGEIHAAGLDVFEQEPLSVDSPLLS
                     MANVVAVPHIGSATHETRYGMAACAVDNLIDALQGKVEKNCVNPHVAD"
     gene            complement(3560..4219)
                     /locus_tag="J1Y83_002709"
     CDS             complement(3560..4219)
                     /locus_tag="J1Y83_002709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family lipoprotein"
                     /protein_id="HBA7630845.1"
                     /translation="MKKRVYLIAAVVSGALAVSGCTTNPYTGEREAGKSAIGAGLGSL
                     VGAGIGALSSSKKDRGKGALIGAAAGAALGGGVGYYMDVQEAKLRDKMRGTGVSVTRS
                     GDNIILNMPNNVTFDSSSATLKPAGANTLTGVAMVLKEYPKTAVNVIGYTDSTGGHDL
                     NMRLSQQRADSVASALITQGVDASRIRTQGLGPANPIASNSTAEGKAQNRRVEITLSP
                     L"
     gene            4372..6705
                     /locus_tag="J1Y83_002710"
     CDS             4372..6705
                     /locus_tag="J1Y83_002710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418007.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin guanine dinucleotide-containing
                     S/N-oxide reductase"
                     /protein_id="HBA7630846.1"
                     /translation="MANSSSRYSVLTAAHWGPMLVETDGETVFSSRGALATGMENSLQ
                     SAVRDQVHSNTRVRFPMVRKGFLASPENPQGIRGQDEFVRVSWDEALELIHHQHKRIR
                     EAYGPASIFAGSYGWRSNGVLHKASTLLQRYMALAGGYTGHLGDYSTGAAQAIMPYVV
                     GGSEVYQQQTSWPLVLEHSDVVVLWSANPLNTLKIAWNASDEQGLSYFSALRDSGKKL
                     ICIDPMRSETIDFFGDKMEWVAPHMGTDVALMLGIAHTLVENGWHDEAFLARCTTGYA
                     VFASYLLGESDGIAKNAEWAAEICGVGAAKIRELAAIFHQNTTMLMAGWGMQRQQFGE
                     QKHWMLVTLAAMLGQIGTPGGGFGLSYHFANGGNPTRRAAVLSSMQGSLPGGTDAVDK
                     IPVARIVEALENPGGAYQHNGMDRHFPDIRFIWWAGGANFTHHQDTNRLIRAWQKPEL
                     VVISECFWTAAAKHADIVLPATTSFERNDLTMTGDYSNQHLVPMKQVVPPRDEARNDF
                     DVFADLSERWEKGGYARFTEGKSELQWLETFYNVARQRGASQQVELPPFAEFWQANQL
                     IEMPENPDSERFIRFADFRRDPQAHPLKTASGKIEIFSQRIADYAYPDCPGHPMWLEP
                     DEWQGNAEPEQLQVLSAHPAHRLHSQLNYSSLRELYAVANREPVTIHPDDAQTRGIQD
                     GDTVRLWNSRGQILAGAVISEGIKPGVICIHEGAWPDLDLTAGGICKNGAVNVLTKDL
                     PSSRLGNGCAGNTALAWLEKYNGPELTLTAFEPPASS"
     gene            complement(6674..7114)
                     /locus_tag="J1Y83_002711"
     CDS             complement(6674..7114)
                     /locus_tag="J1Y83_002711"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HBA7630847.1"
                     /translation="MIREVQRSELPAILQLWLESTTWGHPFIKSSYWRDCIPLVRDAY
                     LANAQNWVWEEDGKLLGFVSIMEGRFLAAMFVAPKAVRRGIGKALMQYVQQRHPHLML
                     EVYQKNQPAIDFYHAQGFHIVDCAWQDETQLPTWIMSWPVVQTL"
     gene            complement(7111..7674)
                     /gene="tag"
                     /locus_tag="J1Y83_002712"
     CDS             complement(7111..7674)
                     /gene="tag"
                     /locus_tag="J1Y83_002712"
                     /EC_number="3.2.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HBA7630848.1"
                     /translation="MERCGWVSQDPLYIAYHDNEWGVPETDSKKLFEMICLEGQQAGL
                     SWITVLKKRENYRACFHQFDPVKVAAMQEEDVERLVQDAGIIRHRGKIQAIIGNARAY
                     LQMEQNGEPFADFVWSFVNHQPQVTQATTLSEIPTSTPASDALSKALKKRGFKFVGTT
                     ICYSFMQACGLVNDHVVGCCCHPGNKP"
     gene            7832..8530
                     /locus_tag="J1Y83_002713"
     CDS             7832..8530
                     /locus_tag="J1Y83_002713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312460.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HBA7630849.1"
                     /translation="MIIKKSGGRWQLSLLASVVISAFFLNTAYAWQQEYIVDTQPGHS
                     TERYTWDSDHQPDYNDILSQRIQSSQRALGLEVNLAEETPVDVTSSMSMGWNFPLYEQ
                     VTTGPVAALHYDGTTTSMYNEFGDSTTTLADPLWHASVSTLGWRVDSRLGDLRPWAQI
                     SYNQQFGENIWKAQSGLSRMTATNQNGNWLDVTVGADMLLNQNIAAYAALSQAENTTN
                     NSDYLYTMGVSARF"
     gene            8759..9967
                     /locus_tag="J1Y83_002714"
     CDS             8759..9967
                     /locus_tag="J1Y83_002714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBA7630850.1"
                     /translation="MTPSNYQRTRWLTLIGTIITQFALGSVYTWSLFNGALSAKLGAP
                     VSQVAFSFGLLSLGLAISSSVAGKLQERFGVKRVTMASGILLGLGFFLTAHSNNLMML
                     WLSAGVLVGLADGAGYLLTLSNCVKWFPERKGLISAFAIGSYGLGSLGFKFIDTHLLE
                     TVGLEKTFVIWGAIVLVMIVFGATLMKDAPKQEVKTSNGVVEKDYTLAESMRKPQYWM
                     LAVMFLTACMSGLYVIGVAKDIAQSLAHLDAISAANAVTVISIANLSGRLVLGILSDK
                     IARIRVITIGQVIALVGMAALLFAPLNAVTFFAAIACVAFNFGGTITVFPSLVSEFFG
                     LNNLAKNYGVIYLGFGIGSICGSIIASLFGGFYVTFYVIFALLILSLALSTTIRQPEQ
                     KMLREAHGSL"
     gene            10291..11982
                     /gene="eptB"
                     /locus_tag="J1Y83_002715"
     CDS             10291..11982
                     /gene="eptB"
                     /locus_tag="J1Y83_002715"
                     /EC_number="2.7.8.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418002.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid A phosphoethanolamine
                     7''-transferase"
                     /protein_id="HBA7630851.1"
                     /translation="MRYIKSITQQKLSFLLAIYIGLFMNGAVFYRRFGSYAHDFTVWK
                     GISAVVELAATVLVTFFLLRLLSLFGRRSWRILASLVVLFSAGASYYMTFLNVVIGYG
                     IIASVMTTDIDLSKEVVGLNFILWLIAVSALPLILIWNNRCRYTLLRQLRTPGQRIRS
                     LAVVVLAGIMVWAPIRLLDIQQKKVERATGVDLPSYGGVVANSYLPSNWLSALGLYAW
                     ARVDESSDNNSLLNPAKKFTYQAPQNVDDTYVVFIIGETTRWDHMGIFGYERNTTPKL
                     AQEKNLAAFRGYSCDTATKLSLRCMFVRQGGAEDNPQRTLKEQNIFAVLKQLGFSSDL
                     YAMQSEMWFYSNTMADNIAYREQIGAEPRNRGKPVDDMLLVDEMQQSLGRNPDGKHLI
                     ILHTKGSHFNYTQRYPRSFAQWKPECIGVDSGCTKAQMINSYDNSVTYVDHFISSVID
                     QVRDKKAIVFYAADHGESINEREHLHGTPRELAPPEQFRVPMMVWMSDKYLENPVNAQ
                     AFAQLKKEADMKVPRRHVELYDTIMGCLGYTSPDGGINENNNWCHIPQAREAAAN"
     gene            12074..12150
                     /locus_tag="J1Y83_002716"
     tRNA            12074..12150
                     /locus_tag="J1Y83_002716"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:12108..12110,aa:Pro,seq:cgg)
     gene            12894..14501
                     /gene="dppA"
                     /locus_tag="J1Y83_002717"
     CDS             12894..14501
                     /gene="dppA"
                     /locus_tag="J1Y83_002717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter substrate-binding
                     protein DppA"
                     /protein_id="HBA7630852.1"
                     /translation="MRISLKKSGMLKLGLSLVAMTVAASVQAKTLVYCSEGSPEGFNP
                     QLFTSGTTYDASSVPLYNRLVEFKIGTTEVIPGLAEKWEVSEDGKTYTFHLRKGVKWH
                     DNKEFKPTRELNADDVVFSFDRQKNAQNPYHKVSGGSYEYFEGMGLPELISEVKKVDD
                     NTVQFVLTRPEAPFLADLAMDFASILSKEYADAMMKAGTPEKLDLNPIGTGPFQLQQY
                     QKDSRIRYKAFDGYWGTKPKIDTLVFSITPDASVRYAKLQKNECQVMPYPNPADIARM
                     KQDKSINLMEMPGLNVGYLSYNVQKKPLDDVKVRQALTYAVNKDAIIKAVYQGAGVSA
                     KNLIPPTMWGYNDDVQDYTYDPEKAKALLKEAGLEKGFSIDLWAMPVQRPYNPNARRM
                     AEMIQADWAKVGVQAKIVTYEWGEYLKRAKDGEHQTVMMGWTGDNGDPDNFFATLFSC
                     AASEQGSNYSKWCYKPFEDLIQPARATDDHNKRVELYKQAQVVMHDQAPALIIAHSTV
                     FEPVRKEVKGYVVDPLGKHHFENVSIE"
     gene            14652..15671
                     /gene="dppB"
                     /locus_tag="J1Y83_002718"
     CDS             14652..15671
                     /gene="dppB"
                     /locus_tag="J1Y83_002718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter permease DppB"
                     /protein_id="HBA7630853.1"
                     /translation="MLQFILRRLGLVIPTFIGITLLTFAFVHMIPGDPVMIMAGERGI
                     SPERHAQLLAELGLDKPMWQQYLHYIWGVMHGDLGISMKSRIPVWEEFVPRFQATLEL
                     GVCAMIFATAVGIPVGVLAAVKRGSIFDHTAVGLALTGYSMPIFWWGMMLIMLVSVHW
                     NLTPVSGRVSDMVFLDDSNPLTGFMLIDTAIWGEDGNFIDAVAHMILPAIVLGTIPLA
                     VIVRMTRSSMLEVLGEDYIRTARAKGLTRMRVIIVHALRNAMLPVVTVIGLQVGTLLA
                     GAILTETIFSWPGLGRWLIDALQRRDYPVVQGGVLLVATMIILVNLLVDLLYGVVNPR
                     IRHKK"
     gene            15681..16583
                     /gene="dppC"
                     /locus_tag="J1Y83_002719"
     CDS             15681..16583
                     /gene="dppC"
                     /locus_tag="J1Y83_002719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010426221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter permease DppC"
                     /protein_id="HBA7630854.1"
                     /translation="MSQVTENKVISAPVPMTPLQEFWHYFKRNKGAVVGLVYVVIVLF
                     IAIFANWIAPYNPAEQFRDALLAPPAWQEGGSMAHLLGTDDVGRDVLSRLMYGARLSL
                     LVGCLVVVLSLIMGVILGLIAGYFGGLVDNIIMRVVDIMLALPSLLLALVLVAIFGPS
                     IGNAALALTFVALPHYVRLTRAAVLVEVNRDYVTASRVAGAGAMRQMFINIFPNCLAP
                     LIVQASLGFSNAILDMAALGFLGMGAQPPTPEWGTMLSDVLQFAQSAWWVVTFPGLAI
                     LLTVLAFNLMGDGLRDALDPKLKQ"
     gene            16594..17577
                     /gene="dppD"
                     /locus_tag="J1Y83_002720"
     CDS             16594..17577
                     /gene="dppD"
                     /locus_tag="J1Y83_002720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding protein"
                     /protein_id="HBA7630855.1"
                     /translation="MALLNVDKLSVHFGDESAPFRAVDRISYSVKQGEVVGIVGESGS
                     GKSVSSLAIMGLIDYPGRVMAEKLEFNGQDLQRISEKERRNLVGAEVAMIFQDPMTSL
                     NPCYTVGFQIMEAIKVHQGGNKSTRRQRAIDLLNLVGIPDPASRLDVYPHQLSGGMSQ
                     RVMIAMAIACRPKLLIADEPTTALDVTIQAQIIELLLELQQKENMALVLITHDLALVA
                     EAAHKIIVMYAGQVVETGDAHAIFHAPRHPYTQALLRALPEFAQDKERLASLPGVVPG
                     KYDRPNGCLLNPRCPYATDKCRAEEPALNMLADGRQSKCHYPLDDAGRPTL"
     gene            17574..18578
                     /gene="dppF"
                     /locus_tag="J1Y83_002721"
     CDS             17574..18578
                     /gene="dppF"
                     /locus_tag="J1Y83_002721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP binding subunit
                     DppF"
                     /protein_id="HBA7630856.1"
                     /translation="MSTHEATSQQPLLQAIDLKKHYPVKKGMFAPERLVKALDGVSFN
                     LERGKTLAVVGESGCGKSTLGRLLTMIETPTGGELYYQGQDLLKHDPQAQKLRRQKIQ
                     IVFQNPYGSLNPRKKVGQILEEPLLINTSLSKEQRREKALSMMAKVGLKTEHYDRYPH
                     MFSGGQRQRIAIARGLMLDPDVVIADEPVSALDVSVRAQVLNLMMDLQQELGLSYVFI
                     SHDLSVVEHIADEVMVMYLGRCVEKGTKDQIFNNPRHPYTQALLSATPRLNPDDRRER
                     IKLTGELPSPLNPPPGCAFNARCRRRFGPCIQLQPQLKDYGGQLVACFAVDQDENPQR
                     "
     gene            complement(18608..19879)
                     /locus_tag="J1Y83_002722"
     CDS             complement(18608..19879)
                     /locus_tag="J1Y83_002722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAAAP family serine/threonine permease"
                     /protein_id="HBA7630857.1"
                     /translation="MQHNTLPKHDQKLPFTRYDFGWVLLCIGMAIGAGTVLMPVQIGL
                     KGIWVFITAAIIAYPATWVVQDIYLKTLSESDSCNDYTDIISHYLGKNWGIFLGVIYF
                     LMIIHGIFIYSLSVVFDSASYLKTFGLTDADLSQSLFYKVAIFAVLVAIASGGERLLF
                     KISGPMVVVKVGIIVVFGFAMIPHWNFANITAFPQASDFFRDVLLTIPFCFFSAVFIQ
                     VLNPMNIAYRKREADKVLATRLALRTHRISYITLIAVILFFAFSFTFSISHEEAVSAF
                     EQNISALALAAQVIPGHIIHITSTVLNIFAVLTAFFGIYLGFHEAIKGIILNLLSRII
                     DTRKINSRMLTLAICAFIVITLTIWVSFRVSVLVFFQLGSPLYGIVSCLIPFFLIYKV
                     SQLEKLRGFKAWMILLYGILLCLSPLLKLIE"
BASE COUNT         4841 a         4965 c         5292 g         5080 t
ORIGIN      
        1 agctagaata gccacgggtg cttgagactg tttgtctcag gtattcaccg aaaggcagac
       61 agagaaaagc cccacctgac tataaatcaa agtgaggccc ctctatgcat agacaccata
      121 aggttagcct cttactcgtt ggaaatcaac acagggggct gggaatgccg cagaaatata
      181 gattactttc tttaatagtg atttgtttca cgcttttatt tttcacctgg atgataagag
      241 attcactgtg tgaattgcat attaaacagg agagttatga gctggcggcg tttttagcct
      301 gcaaattgaa agagtaagcg tcttcggcgg gaaattattc ccgcctttct tacggtgttg
      361 cgcattctca ttgcacccaa atttattctt cacaaaaata ataatagatt ttattacgtg
      421 atcgattatt tatttcctga aaaacaaata aaaaaatccc cgccaaatgg cagggatctt
      481 agattctgtg cttttaagca gagattacag gctggttacg ttaccagctg ccgggccttt
      541 agcgccgctt tcgatggtga aggacacttt ctgaccttcg tccagagatt tgtaaccatc
      601 gttctggata gcagagaagt gtacgaacac gtctttagag ccatcgtcag gagtgatgaa
      661 gccgaagcct ttgtcagcgt tgaaccattt tacgatacca gtcattttac cggacatagt
      721 gtattacctt taataattaa ttgtgccttt cggcgatatg gcgtgcttta cagattttga
      781 agcgttaaag gaatgtgcac tacgaggggt atcaacgata actcttgaag ggacttgcct
      841 tactacactg ctttaatggt ctgtacgtca aaccgttgat gtgcattaag ccacgcattg
      901 gtgggtgatg caacaattat ttttcatatt tatgattaat cggttggaaa acggtcctgt
      961 tatcaggacc gtaaacagaa ataaagtgga taaaagtcta ttccatcaac tgcttactta
     1021 atgccgggtt ggcttgaatc aaacgcatca attttagttc ggcacttgaa ggcttcacgc
     1081 gtctggattc ccattccttt accatggcga ctgatacgcc caaaacccgg gcgaaatcat
     1141 cgatttttaa tcctgtccct tttcgtaact gctcaatttc ggtacaggat gttgttctgt
     1201 gcgtcagggt aattttctgc gtttcatctt taaaaacaat ctgttccagg ctgctcaaca
     1261 gctcatgcat tggatcttta tattccattg aaaactcctc aaatcacact gcgggatcgt
     1321 gaattgcatc gaagctcatt aagaatagtc gggaaaaaca gacacagcgg ggtgttgtca
     1381 gggcatactc tgactatgat tcgattcagc cagtcattta gcgtgactta tagccaccta
     1441 acgatctgat aaatgccgat atatctctta ccactcccgg tttgtaaaga tttcgaaaat
     1501 ctaacgttct gcgaaggcgt aaatctctgg ctacaaaaaa agcaacggca atatcatttt
     1561 ttaacaatga aacaacaaat taaccttgtt tcattttctc catgatttta ccaggaagat
     1621 tacgtagaaa gggtatcctg caagcctgtc ctggactatc cttgtcacgt cagacacgcg
     1681 tgtgtcgttg tgcgcttttt ttgggtgaaa ggagtaagaa aatggcgaca ggaaagtcct
     1741 gctctcgctg gtttgcgcct cttgcggcgt tattaatggt agttagcctg agtgggtgtt
     1801 ttgataaaga aggcgatcag cgtaaagcgt ttatcgactt cctgcagaat acagtgatgc
     1861 gtagcggtga acgtctacca accctgactg ccgatcagaa aaaacagttt ggtccttttg
     1921 tctctgatta cgcgattctg tatggttatt ctcagcaggt gaatcaggcg atggattccg
     1981 gtctgcgccc ggttgtagac agcgttaacg caattcgcgt accgcaggat tacgttacgc
     2041 aaagcggtcc gctgcgtgaa atgaacggtt cgctgggtgt gctggcgcaa cagctgcaaa
     2101 atgcgaagtt gcaggccgat gctgcacact ctgcgttaaa acagagtgat gacctgaaac
     2161 cggtctttga tcaggcattc accaaagtgg taacgacgcc agctgatgca ttgcaaccgt
     2221 tgattccggc ggcacaaact ttcacgcaac aactggtgat ggttggagac tacattgctc
     2281 agcaaggtac tcaggtgagc tttgtcgcaa atggcatcca gttcccgact tcacagcagg
     2341 caagtgaata taacaaactg attgcgccat taccggcaca acatcaggcg tttaatcagg
     2401 cctggactac agcagttact gcaacccaat aaagagtaaa agcccgagcg attatcgggc
     2461 tttactccac gcagtcgcgg cttagtccgc gacgtgcgga ttcacacagt tcttctcaac
     2521 ctttccttgt aacgcatcaa tcaaattatc cacggcacag gcggccatgc cataacgcgt
     2581 ctcatgggtg gcagatccaa tatgcggtac tgcgacgacg ttggccattg agagcaacgg
     2641 cgaatctacg gacagtggct cttgttcgaa gacatccagc ccggcggcgt gaatttcccc
     2701 tttctgcaat gcagcaatca gtgcattttc atcaaccacc gggccacgtc cggcattaat
     2761 gaaaatggcg gaggatttca ttttggcgaa ttgttctgcg ccaaacagat gatgcgtctc
     2821 atccgttaac ggcaggatca ggcaaacgaa atctgactct tgtaacagag tatccaaatc
     2881 gcagtagcgg gcgttgaagc gttcttctgc ttctttatgg tggcgacgcg cgttatagag
     2941 gatgggcatg ttgaagccaa agtgcgcacg ttgtgccagc gccatgccga tccgtcccat
     3001 cccgacaatg cccagtgttt tatggtgaac gtcagtgccg taccagtccg ggcctatgct
     3061 cgcggtccat tcgcctgctt ttacccgttc tgctacctcc acaacccgac gagcggtaga
     3121 caacaccagc gccatcagcg tatcggcgac ggtttctgtt aatacggttg gtgtgtgcat
     3181 cagcagaatt tttcgggcgg taagcgcatc gacatcaaaa ttgtcatagc cgacggagat
     3241 cgttgatgtg gcacgcagtt tcggcatttt ttccagcaat gcggcatcaa cattctcgtt
     3301 tgaacccagc aagccttcag cttcggcaaa aattgctgca ttttgttcga cggtttgtgg
     3361 gctgaggttt gccacctggt gaacggtgaa atgttcttgc aggcgttgca gtaaatcatc
     3421 aggtaaggct ttgtagagga taacggacgg cttcatgctt ctctccattc actgataagg
     3481 cgatcacctt agcctgaaat atgatgaagg aaaactgaag aatgccaggt gatttctgca
     3541 tcacctgaca tgaaagggat tacagtgggc ttaaggtaat ttctacacgg cggttttgcg
     3601 ccttaccttc tgcggtgctg ttgctggcga ttgggttagc cgggccaagg ccctgagtac
     3661 ggatgcggct ggcgtccacg ccttgggtga tcaacgcgct ggcaacggaa tccgcacgct
     3721 gctgggagag acgcatgttc aggtcgtgac cccccgtgct gtcggtataa ccaatcacat
     3781 taaccgccgt tttcggatac tctttcagta ccattgccac gccggtcagg gtgttagcgc
     3841 ccgccggttt cagggttgcg ctgctgctgt cgaaggtcac attgttcggc atattgagga
     3901 taatgttatc cccgctgcgg gttacgctaa caccagtgcc gcgcattttg tcacgcagct
     3961 tcgcttcctg cacatccatg taataaccaa cgccgccgcc cagagctgcg cctgctgctg
     4021 cgccaatgag cgcgccttta ccgcgatctt tcttcgaaga agagagcgca ccaatacccg
     4081 caccaacgag agagcccaga cctgcgccga tagcagattt acctgcttcg cgttcgccgg
     4141 tgtaagggtt agttgtacag ccagataccg ccagagcgcc actcactacg gcggcaataa
     4201 gataaacacg tttcttcatt gttaatcctt aataaccttt ttattctttg ccacgggttc
     4261 cgtggcggga gattatgccg cgtgagcatg aagattattc ctgggaatac tcggaaattt
     4321 gtaagtaata tttaactgct caatacatct aacctttcag gagtcttcgg tttggccaac
     4381 tcatcctcac gatattccgt tctgactgcc gcccactggg ggcctatgct ggttgaaacc
     4441 gacggcgaaa ccgtgtttag ctcgcgtggc gcgttagcca caggaatgga aaactccttg
     4501 cagagcgcgg ttcgcgacca ggttcacagc aatacgcggg tgcgatttcc aatggtgcgc
     4561 aaaggctttc ttgcgtcacc ggaaaatccg caaggcattc gtgggcagga tgaatttgtt
     4621 cgcgtgagtt gggatgaggc gctggagctt attcaccatc aacataaacg cattcgtgag
     4681 gcttatggcc cggcatcgat ttttgctggt tcctacggct ggcgttcaaa cggcgtgctg
     4741 cataaggcct cgacattatt acaacgctat atggcgctgg caggcggtta tactgggcat
     4801 ctgggggatt attcgaccgg cgcggcacag gcgatcatgc cgtatgtcgt gggtggcagc
     4861 gaagtttatc aacagcaaac cagttggccg ctggtgctgg aacatagcga tgtcgtggtg
     4921 ctgtggagtg ctaacccact caatacgctg aaaattgcgt ggaatgcatc cgatgagcag
     4981 gggctttctt acttttccgc actgcgtgac agcgggaaaa agctgatctg cattgatcca
     5041 atgcgatcgg aaaccatcga tttctttggc gataaaatgg agtgggtggc accacacatg
     5101 ggcaccgatg ttgcgttgat gctggggatc gcccatacgc tggtggaaaa tggttggcac
     5161 gacgaagcgt ttctggcgcg ttgcaccaca ggttatgccg tcttcgcctc ttatttgctg
     5221 ggcgagagtg acggtatagc gaaaaacgcc gaatgggcgg cagagatttg tggtgttggc
     5281 gcagcgaaaa tccgcgagct ggcggctatt ttccaccaaa atacaaccat gctgatggca
     5341 ggctggggaa tgcagcgcca acagtttggt gagcaaaaac actggatgct cgtcacgctg
     5401 gcggcaatgt tggggcaaat cggcacaccc ggcggcggtt ttggtctttc ttaccatttt
     5461 gccaatggtg gtaaccccac gcgccgcgct gcggtgctct cttccatgca gggtagcttg
     5521 ccgggtggca ccgatgcggt agataaaatc cctgtcgccc gcattgttga agcactggaa
     5581 aaccctggtg gcgcatatca acataacggt atggaccgac atttcccgga tattcgtttt
     5641 atctggtggg cgggtggtgc caactttact catcatcagg ataccaatcg cctgatccgt
     5701 gcctggcaaa aaccggagct ggtggtgatc tccgaatgct tctggacggc tgcggcgaaa
     5761 cacgcggata tcgttctgcc tgcgacaacc tcttttgagc gtaatgatct caccatgacc
     5821 ggtgattaca gtaatcagca tctggtgccg atgaagcaag tggtgccgcc acgcgatgaa
     5881 gcgcgtaatg attttgatgt ttttgccgac ttgagtgaac gttgggagaa gggcggttat
     5941 gcacgtttta cggaaggaaa aagtgagctg caatggctgg aaacgtttta taacgttgcc
     6001 cggcagcgcg gggcaagcca gcaggttgaa ttgccgccat ttgctgagtt ctggcaagcc
     6061 aaccagttaa ttgagatgcc ggaaaacccg gacagtgagc ggtttattcg cttcgccgat
     6121 tttcgccgcg atccgcaggc gcatccatta aaaaccgcca gcggtaagat tgaaatcttc
     6181 tcgcagcgta ttgccgatta cgcttatccg gattgcccgg ggcatccaat gtggctggag
     6241 ccggacgaat ggcagggcaa tgccgaaccg gaacagttgc aggtactttc tgctcatccg
     6301 gcgcatcgtc tgcacagcca gctgaattac agttctctgc gcgaattgta cgcggtggca
     6361 aatcgtgagc ctgtcaccat tcatcctgac gatgcccaga cgcgcggcat acaagatggc
     6421 gatactgttc ggctgtggaa ctcacgcggg caaattcttg ccggcgcggt cattagcgag
     6481 ggaataaaac ctggcgtgat ttgtattcac gaaggcgcat ggccggattt ggatttaacc
     6541 gctggcggca tttgtaaaaa cggcgcagtg aacgtgctga ccaaagatct ccccagctcg
     6601 cggctgggga atggctgtgc gggtaatacg gcgctggcat ggctggaaaa atacaacggt
     6661 ccggaactga cacttacagc gtttgaacca ccggccagct cataatccag gtgggtagtt
     6721 gggtttcatc ctgccatgcg caatcgacaa tgtgaaaacc ctgcgcatgg tagaaatcta
     6781 tcgccggttg atttttttga taaacctcca gcatcagatg gggatggcgc tgctgcacat
     6841 actgcatcag cgccttacca ataccgcgcc tgacggcctt cggtgcgaca aacattgctg
     6901 ccagaaatcg gccttccata atgctgacaa aaccgagaag cttaccgtct tcttcccaga
     6961 cccagttttg cgcgttggca agatacgcat cccgcaccag cggaatgcag tcacgccagt
     7021 aactcgattt tataaaggga tgcccccagg ttgtactttc cagccacagt tggaggatcg
     7081 cggggagttc tgaacgttgc acttcccgaa tcatggttta tttcccggat ggcaacagca
     7141 gccaaccaca tgatcattca ccagcccaca tgcctgcata aaggagtaac agattgtggt
     7201 accgacaaac ttaaaaccac gtttttttag tgccttagat agggcgtcgg aggcgggtgt
     7261 agatgtgggg atttcgctca acgttgtggc ttgtgtcacc tgtggttgat gattgacaaa
     7321 cgaccagaca aagtcggcaa acggttcgcc gttctgttcc atttgcaggt acgcccgcgc
     7381 attaccaata attgcctgaa ttttcccacg atggcggata atcccggcgt cctgtaccag
     7441 tctttcgaca tcctcttcct gcattgcggc aactttcacc ggatcgaact gatgaaagca
     7501 tgcgcgatag ttttcgcgtt ttttgaggac ggtgatccac gataatccag cctgctgccc
     7561 ttccaggcag atcatttcga acagtttttt actgtcagtt tcaggcacgc cccattcatt
     7621 atcatggtag gcaatataaa gcgggtcctg actcacccag ccgcaacgtt ccatactttc
     7681 cctcgctatg tacccgatcg taaaaatttc agccgctttt cttgacgcat cggtgaaaaa
     7741 gtcaatattt aatacagggc tactatgccc gatatccttc atacaatgac aataatattg
     7801 ccgaattcgg ctcttccctg gagtcgtgtt aatgatcata aaaaaaagcg gtggtcgctg
     7861 gcagctaagc ctgcttgcga gcgtggtaat cagtgccttt tttctcaaca cagcttacgc
     7921 ctggcaacaa gaatatatcg ttgatacgca acccggacat tccacagagc gttacacctg
     7981 ggatagcgat catcaacctg attacaacga tattttgtca caacgtattc aaagtagcca
     8041 aagggcgctg ggactggaag tcaatctggc ggaagaaacc cctgtggatg tgaccagcag
     8101 tatgagtatg ggctggaatt ttcctttata tgagcaggtt acaaccggcc cggtcgcggc
     8161 attacattac gatggcacaa ctacctcgat gtataacgag tttggcgaca gtactaccac
     8221 gctggccgat ccgttatggc atgccagcgt gagtacctta ggctggcgtg ttgactcccg
     8281 gcttggcgat cttcgaccct gggcgcaaat cagctataac cagcaatttg gcgagaatat
     8341 ctggaaggcg caatcaggcc tgagccggat gacggcgaca aaccagaacg gcaactggct
     8401 ggatgtcacc gtaggcgctg atatgttgct caatcaaaat attgctgcct atgctgcgct
     8461 atctcaggca gaaaatacca ctaacaatag cgactatctg tatacgatgg gggttagcgc
     8521 cagattttaa cgttatagat acaattgaaa cccataaata acaactaatg tggcgatagt
     8581 ggggactaac ttactaaata ataaatttgg cgaataattg tcgagtcatt cattcctgaa
     8641 ctagtgcatt tcattccgtt ctgatggcat ttcatgccgt ttttccccag tcataaagcg
     8701 cacttcgtta tggttgtcag cagagatttt tcctttttat tacagcagga atactgccat
     8761 gacaccttca aattatcagc gtactcgctg gctgacactc atcggtacta tcattaccca
     8821 gtttgctctg gggtcggttt atacctggag tctgtttaac ggtgcgcttt ctgccaagct
     8881 gggggctcct gtaagccagg ttgctttctc tttcggcttg ttaagtctgg ggctggcaat
     8941 ttcgtcttct gttgcgggca aattgcagga acgttttggt gttaaacgcg tcactatggc
     9001 ttccggcatt ttgctgggat taggtttctt cctgacagca cattccaaca acctgatgat
     9061 gctgtggtta agcgccggtg tgttggttgg tctggcggat ggtgcgggtt acctgttgac
     9121 gctctctaac tgcgtgaagt ggtttccgga gcgtaaggga cttatctctg cttttgctat
     9181 cggttcttat ggtctgggca gcctgggttt taaatttatc gacacgcacc tgctcgaaac
     9241 ggtgggtctg gaaaaaacct ttgttatttg gggcgcgatt gtactggtga tgattgtctt
     9301 tggcgcaacg ttaatgaaag atgcgccgaa gcaggaagtg aaaaccagca atggtgtggt
     9361 ggagaaggac tacaccctgg cagagtcgat gcgtaaaccg cagtactgga tgttagcggt
     9421 tatgttcctg actgcgtgca tgagtggtct gtatgtgatt ggtgtagcga aagatatcgc
     9481 tcaaagtctg gcgcatcttg atgcaatttc cgcagccaat gctgtgacgg ttatttccat
     9541 cgccaacctt tctggtcgtc tggtgctggg cattctgtct gacaaaatcg cccgtatccg
     9601 tgttatcacc attggtcagg tgattgcgct ggtgggtatg gcggccctgc tgtttgcacc
     9661 attgaatgca gtgacgttct ttgcagcgat tgcctgtgtg gcatttaact ttggtggcac
     9721 gattactgtg ttcccgtcgc tggttagtga gttcttcggc ctcaataacc tggcgaaaaa
     9781 ctacggcgtc atttacctcg gtttcggtat cggcagcatt tgtggttcga ttatcgcttc
     9841 actgtttggc ggattctatg tgactttcta cgtgattttc gccctgctga ttctgtcact
     9901 ggcgctttct acgaccattc gccagccaga gcagaaaatg ttgcgtgagg cgcatggctc
     9961 cctttaatca tctgccgcga gctactgggc ttcagcttca gtgtgcatca aacgcatcaa
    10021 cgaaatgtat aaacaataac gcgtaaaatt tgggccagtt agctggccct ttttactgct
    10081 ttttgtaacg ctaaagtctc ttttcaaact tgcatttttg taaatttgtg cttcgtgcac
    10141 actctttccc cacacttttt ccctttgctg tggtctactt attcgcgcgt gtagatttta
    10201 cttatctgac tacctccgca cttttcccct gcctggcctg aaaagccact aagcagggtg
    10261 ttatcacctg tttgtccagg gtttgtttgc atgagataca tcaaatcgat tacacagcag
    10321 aagctgagct ttttgcttgc aatctatatt ggccttttta tgaatggcgc ggttttttac
    10381 cgccgcttcg gcagctatgc gcacgatttt accgtctgga aaggcatttc tgctgttgtt
    10441 gaactggccg ccaccgtact ggtgaccttc tttttactgc gtcttctttc gctgtttggc
    10501 cgccgcagct ggcgtattct ggcatcgctg gtggtgctct tttccgcagg tgccagctat
    10561 tacatgacct tccttaatgt agtcattggt tatggcatca tcgcatccgt catgaccacc
    10621 gatatcgacc tgtcaaaaga agttgttggt ctgaacttta ttctctggtt aatcgccgta
    10681 agcgcgttgc ctcttatcct tatctggaat aaccgctgtc gttacacctt gctccgacaa
    10741 ctgcgaaccc cagggcaacg tattcgcagc ctggcggtcg tcgtactggc aggtattatg
    10801 gtttgggcac cgattcgttt gctggatatc cagcagaaga aagtggagag ggcgacaggt
    10861 gttgatttgc cgagttatgg cggcgtcgtc gcgaactctt atctgccatc aaactggctt
    10921 tcggcattag gtctttatgc ctgggcgcgg gtcgatgaat cttccgataa caattctctc
    10981 cttaaccctg cgaagaagtt tacgtaccag gctccacaaa acgttgatga tacatatgtc
    11041 gttttcatta ttggtgaaac cacgcgctgg gatcatatgg gcattttcgg ctatgaacgc
    11101 aataccacac cgaagctggc tcaggagaaa aatctggcgg cgttccgtgg ttactcctgt
    11161 gatactgcaa ccaaactctc actgcgttgc atgtttgtac gtcagggtgg agcagaagat
    11221 aatccgcagc gcacattaaa agaacagaac attttcgcgg tattgaagca gttaggattc
    11281 agttctgatc tctacgctat gcaaagcgaa atgtggttct acagcaatac gatggcggac
    11341 aacattgctt atcgtgagca gattggtgcg gagccacgta accgtggcaa gccggtagat
    11401 gatatgttgc tggtagacga aatgcagcaa tcgctggggc gcaacccgga tggtaagcat
    11461 ctgatcattc tgcataccaa aggctcacac tttaactaca cccagcgtta tccgcgcagc
    11521 tttgcgcagt ggaagccgga atgtattggt gttgatagcg gctgtaccaa agcgcagatg
    11581 atcaactcat acgacaactc ggtgacctat gtggatcact ttatttccag cgtgattgat
    11641 caggttcgcg ataagaaagc gattgtgttc tatgcagctg accacggtga gtcaattaat
    11701 gaacgcgagc acctgcacgg cacgccgcgt gaactggcac cgccggaaca gttccgtgtg
    11761 ccgatgatgg tctggatgtc ggataaatat ctggaaaatc cggtcaatgc gcaggcattt
    11821 gcgcagctga aaaaagaagc cgacatgaaa gtgccacgcc gtcatgtaga gctgtacgac
    11881 accatcatgg gttgtcttgg ctatacttca ccggatggtg gaattaacga aaacaacaac
    11941 tggtgccaca tcccgcaggc aagagaggcg gcggctaact aagaggcctg ctgactttct
    12001 cgccgatcaa aaggcatttt gctattaagg gattgacgag ggcgtatctg cgcagtaaga
    12061 tgcgccccgc attcggtgat tggcgcagcc tggtagcgca cttcgttcgg gacgaagggg
    12121 tcggaggttc gaatcctcta tcaccgacca aattcgaaaa gcctgctcaa cgagcaggct
    12181 tttttgcatc tgtaactcat gaggaagaga acctccgggg gcggggaggt tcgactcgac
    12241 gtaggcctga taagacgcgc cagcgtcgca tcaggcaaat gtaccggccc attgtcggat
    12301 gcggcgcgac cgccttatcc gatctacaca ccacacattt ccctgccaaa tttgcgcccc
    12361 atatctcact atccattaac ggatttgtga cagaaaccat tgtctttttt tatatttgct
    12421 taaatctttt tttgcgttac ttatggctgg cgctggcatt ttccgctgtg cgttttagcg
    12481 ttcatggtat taatcgctca gatatccacc tttacctcag atttttttca ttaaatgcat
    12541 aaaacttaat caccaaatgt tgctaaatat taagctgatt gttctggttg tgttggcgta
    12601 gcacaaattt ctctgctgca aatagctgtt tgaagttttt ttaatctttg tttgtggatt
    12661 ctcaccgttg gtgtaaatcc cggttggctg tattgacgtt ttcacattct gttgacagat
    12721 tgtaggtcac gaggggcatt ttatggagga tccgcactgt tacactgatg ttaattagta
    12781 cggcatcccc acctcataac gttgacccga ccgggcaaaa aacaaaaaag gtcaggcagc
    12841 gacaacccac tgcaaagggt taaaacaaca aacatcacaa ttggagcaga ataatgcgta
    12901 tttccttgaa aaagtcaggg atgctgaagc ttggtctcag cctggtggct atgaccgtcg
    12961 cagcaagtgt tcaggctaaa actctggttt attgctcaga aggatctccg gaagggttta
    13021 acccgcagct gtttacctcc ggcaccacct atgacgcctc ttccgtaccg ctttataacc
    13081 gtctggttga atttaaaatc ggcaccactg aagtgatccc gggcctcgct gaaaagtggg
    13141 aagtcagcga agacggtaaa acctatacct tccatctgcg taaaggtgtg aagtggcacg
    13201 acaataaaga attcaaaccg acgcgcgaac tgaatgccga tgacgtagtg ttctcgttcg
    13261 atcgtcagaa aaacgcgcaa aacccgtacc ataaagtctc tggcggcagc tacgaatact
    13321 tcgaaggcat gggcttacca gagctgatca gcgaagtgaa aaaggtggac gataacaccg
    13381 ttcagtttgt gctgactcgc ccggaagcgc cgttcctcgc tgacctggca atggacttcg
    13441 cctctattct gtcaaaagaa tatgctgacg cgatgatgaa agccggtaca ccggaaaaac
    13501 tggacctcaa cccaatcggt accggcccgt tccagttaca gcagtaccag aaagattccc
    13561 gtattcgcta taaagcgttt gatggctact ggggcaccaa accgaagatc gatacgctgg
    13621 tcttctctat tactcctgac gcttccgtgc gttacgcgaa attgcagaag aacgaatgcc
    13681 aggtgatgcc gtacccgaac ccggcagata tcgcccgcat gaagcaggat aaatccatca
    13741 acctgatgga aatgccgggg ctgaacgtcg gttatctctc gtataacgtg cagaaaaaac
    13801 cactggatga cgtgaaagtt cgccaggctc tgacctacgc ggtgaacaaa gacgccatca
    13861 tcaaagcggt ttatcagggc gcgggcgtat cagcgaaaaa cctgatcccg ccaaccatgt
    13921 ggggctataa cgacgacgtt caggactaca cttacgatcc tgaaaaagcg aaagccttgc
    13981 tgaaagaagc gggtctggaa aaaggtttct ccatcgacct gtgggcaatg ccggtacaac
    14041 gtccgtataa cccgaatgct cgccgcatgg cggagatgat tcaggcagac tgggcgaaag
    14101 tgggcgtgca ggccaaaatc gtcacctacg aatggggtga gtacctcaag cgtgcgaaag
    14161 atggcgagca tcagacggta atgatgggct ggactggcga taacggggat ccggataact
    14221 tcttcgccac cctgttcagc tgcgccgcct ctgaacaagg ctccaactac tcaaaatggt
    14281 gctacaaacc gtttgaagat ctgattcaac cggcgcgtgc taccgacgac cacaataaac
    14341 gtgttgaact gtacaaacaa gcacaggtcg tgatgcacga tcaggctccg gcactgatca
    14401 tcgctcactc caccgtgttt gaaccggtac gcaaagaagt taaaggctat gtggttgatc
    14461 cattaggcaa acatcacttc gaaaatgtct ctatcgaata attaaaagcc atacccggtg
    14521 gcgctgtgcc tctgcgtgca gcgccatccg gcagcaatac ttcattccct gttccacaag
    14581 ggcgggaatt gatttgtgag caatacagac acgcagttcc aggctgcggg tcattataga
    14641 gaatccgggt tatgttgcag tttattctcc gacgtttggg actcgtcatc cccacgttta
    14701 tcggtattac ccttctcaca tttgcctttg tccacatgat cccgggcgat ccggtgatga
    14761 tcatggcggg cgaacgtggg atctccccag agcgtcacgc gcaattgctg gctgaactcg
    14821 gcttagataa accgatgtgg cagcagtatc tccattacat ttggggcgta atgcacggtg
    14881 atttaggcat ttcgatgaaa agccgaattc cggtatggga agagttcgtg ccgcgctttc
    14941 aggccacgct ggaacttggc gtctgcgcga tgatttttgc gacggcagtc ggtattccgg
    15001 ttggtgtgct ggctgcggtt aaacgtggtt ccattttcga tcacacagcg gttggcctgg
    15061 cgctgaccgg atactcgatg ccaattttct ggtggggcat gatgctgatc atgctggttt
    15121 cggtgcactg gaacctgacg cctgtctccg gtcgtgtgag cgacatggtg ttcctcgatg
    15181 attccaatcc gttaaccggt tttatgctga tcgacaccgc catctggggt gaagacggta
    15241 actttatcga tgccgtcgcc catatgatcc tgcccgccat tgtgctgggt accatcccgc
    15301 tggcggtcat tgtgcgtatg acgcgctcct cgatgctgga agtgctgggc gaagattata
    15361 tccgcaccgc gcgtgccaaa ggtcttaccc ggatgcgggt gattatcgtc catgcgctgc
    15421 gtaacgcgat gctgccggtg gtgaccgtta tcggcctgca ggtgggaaca ttgctggcgg
    15481 gggcgattct gactgaaacc atcttctcgt ggcccggtct ggggcgctgg ttgattgacg
    15541 cactgcaacg ccgcgattat ccggtagtgc agggcggcgt attgctggtg gcgacgatga
    15601 ttatcctcgt caacctgctg gtcgatctgc tgtacggcgt ggtgaacccg cgtattcgtc
    15661 ataagaagta aggggacatc atgtcacagg ttactgaaaa taaagtgatt agcgcaccgg
    15721 tgccgatgac cccgttacag gagttctggc actattttaa acgcaacaaa ggcgcggttg
    15781 tcgggctggt ttacgtcgtc atcgtgctgt ttatcgcgat ctttgccaac tggattgcac
    15841 cctataaccc agcggaacag ttccgcgatg cactgctcgc cccgccagcc tggcaggaag
    15901 gcggcagcat ggcgcacttg ctgggcactg atgacgtggg gcgtgatgtg ctgtcgcggc
    15961 tgatgtacgg cgcgcgcttg tcgctgctgg ttggctgtct ggtagtggtg ttatcgctga
    16021 ttatgggcgt tattctcggc ctgatcgctg gttactttgg cggcctggtc gataacatca
    16081 tcatgcgtgt ggtcgatatc atgctggcgc tgccaagtct actgctggcg ttggtgctgg
    16141 tggcgatttt cggcccgtcg attggtaacg ccgcgctggc gctaaccttc gttgcgctgc
    16201 cgcattatgt gcgcttaacc cgcgccgccg tgctggtgga agttaaccgc gattacgtca
    16261 ccgcgtctcg cgtggcgggt gccggggcaa tgcgccagat gtttattaac atcttcccga
    16321 actgccttgc gccgctgatt gttcaggcgt cgctcggttt ctctaacgcc attctcgata
    16381 tggctgctct tggtttcctc ggcatgggcg cgcagccgcc aacgccggag tggggcacca
    16441 tgctctctga tgtgttgcag ttcgcgcaaa gcgcctggtg ggtcgtgacc ttcccgggtc
    16501 tggcgatcct gctgacggtg ctggcattta acctgatggg tgacggtctg cgtgacgcgc
    16561 tcgatcccaa actgaagcag taagaggttc gagatggcgt tattaaatgt agataaatta
    16621 tcggtgcatt tcggcgacga aagcgcaccg ttccgcgccg tagaccgcat cagctacagc
    16681 gtaaaacagg gtgaagtggt cgggattgtg ggtgagtccg gctctggtaa atcggtcagt
    16741 tcgctggcaa ttatggggct gattgattat ccgggccgcg taatggcgga aaagctggag
    16801 tttaacggtc aggatttgca gcgtatctca gaaaaagagc gccgcaacct ggtgggtgcc
    16861 gaagtggcaa tgatcttcca ggacccgatg accagcctta acccgtgcta caccgtgggt
    16921 ttccagatta tggaagcgat taaggtgcat cagggcggca acaaaagtac ccgccgtcag
    16981 cgagcgattg acctgctgaa tctggtcggt attcccgatc cggcatcgcg tctggatgtt
    17041 tacccgcatc agctttccgg cggcatgagc cagcgcgtga tgatcgccat ggcgattgcc
    17101 tgtcgaccaa aactgctgat tgccgatgaa ccgaccactg cgctggacgt gaccatccag
    17161 gcgcaaatca tcgaactgct gctggagttg cagcagaaag agaacatggc gctggtgttg
    17221 atcactcatg acctggcgct ggtggcggaa gcggcacata aaatcatcgt gatgtatgca
    17281 ggccaggtag tagaaacggg cgatgcgcac gctatcttcc atgcgccgcg tcacccgtat
    17341 actcaggcat tgctgcgtgc gctgccggaa tttgctcagg acaaagaacg tctggcgtcg
    17401 ttgccaggtg tcgttcccgg caagtacgac cgcccgaacg gctgcctgct taacccgcgc
    17461 tgcccctacg ccactgacaa atgccgtgct gaagaaccgg cgctgaatat gctcgctgac
    17521 gggcgtcagt ccaaatgcca ttacccactt gatgatgccg ggaggccgac actatgagta
    17581 cgcatgaggc cacctcgcaa caaccgctgt tgcaggctat cgacctgaaa aaacattatc
    17641 cggtgaagaa aggcatgttc gcgccggaac gtctggtgaa ggcgctggat ggcgtttcgt
    17701 ttaaccttga acgtggcaaa acgctggcag tagtaggtga atctggctgc ggtaaatcga
    17761 ccctcggtcg gttgctgacg atgattgaaa cgcccaccgg cggcgaactg tattaccagg
    17821 ggcaggatct gctcaagcac gatccgcagg cgcagaagct acgtcggcag aaaatccaga
    17881 ttgtcttcca gaacccttac ggttcgctga atccgcgtaa aaaagttggg cagattctcg
    17941 aagagccgct gctgatcaac accagcttaa gcaaagaaca gcgtcgggaa aaagccctgt
    18001 cgatgatggc gaaagtcggc ctgaaaaccg agcattacga ccgctatcca catatgttct
    18061 ccggcggtca gcgtcagcgt atcgccattg cccgaggtct gatgctcgac ccggatgtgg
    18121 tgattgccga tgagccggtt tccgcgctgg acgtctcagt gcgtgcgcag gtgttgaatc
    18181 tgatgatgga tttgcagcag gaactggggc tatcttatgt ctttatctcg cacgatctgt
    18241 cggtggtgga gcacattgct gatgaagtga tggtgatgta cctgggccgc tgcgtggaga
    18301 aggggacgaa ggaccaaatc ttcaataacc cgcgccaccc gtacactcag gcattgctct
    18361 ccgcgacacc acgcctgaac ccggacgatc gtcgcgaacg catcaaactt accggtgaac
    18421 tgccaagccc gctgaatcca ccgccaggct gcgccttcaa cgcccgctgt cgtcggcgct
    18481 tcggcccctg catccagttg cagccgcagc taaaagacta cggcggtcaa ctggtagcct
    18541 gttttgccgt tgatcaggat gaaaatccgc agcgttaact cacaaaaccg gggccatgcg
    18601 ctccggttta ctcaatcaac ttcaacagtg gcgacaagca taacaaaatg ccgtacagca
    18661 gaatcatcca ggctttaaac ccgcgaagtt tttccagttg tgagacttta tagatcagga
    18721 aaaacggaat gaggcacgac acaatgccat ataacgggct tcccaactga aagaacacca
    18781 gtaccgatac acgaaacgaa acccaaatcg tcaacgtaat gacgataaaa gcgcagatcg
    18841 ccagagtcag catgcgtgag ttaattttcc tggtatcaat aattcggctt aacagattga
    18901 gaataatgcc tttaatggcc tcgtggaaac cgagataaat gccaaagaat gcagtcagta
    18961 cggcaaagat attcagcacc gtagaggtga tatgaatgat atgcccaggg atcacctgcg
    19021 cggccagtgc cagtgccgag atattttgtt caaaggcaga aacggcttct tcgtggctaa
    19081 ttgagaaggt aaacgaaaag gcaaaaaaca agatcaccgc aatgagcgtg atatagctaa
    19141 tccggtgggt acgcagcgcg agccgggttg ccagtacttt atccgcttcc cgtttacgat
    19201 aggcaatatt cattgggtta agtacctgaa taaataccgc ggaaaagaag caaaatggaa
    19261 tagtaagcag gacatcgcgg aaaaagtcgg aggcttgcgg gaaggcggtt atattggcga
    19321 aattccagtg cgggatcatc gcaaaaccga acacgacaat aatccccact ttgaccacca
    19381 ccattggtcc ggaaatctta aacagtaatc gttcaccacc agacgcaatc gccaccagta
    19441 cggcgaaaat ggcgacttta taaaatagag attgagaaag atcggcatcg gttaaaccga
    19501 aggttttcag gtacgaggcg ctgtcgaaaa ccacggagag agagtagata aaaatcccgt
    19561 ggataatcat caaaaagtag ataaccccga ggaaaattcc ccagttcttc cccaggtaat
    19621 gactgataat atcggtatag tcattacagg aatcgctttc agaaagggtt tttaaataaa
    19681 tgtcctgcac tacccaggtg gcaggataag caatgatcgc tgcggtaata aatacccaaa
    19741 ttcccttcaa gccaatttgt actggcatca gcacggttcc ggcaccaatc gccatgccta
    19801 tgcataataa aacccagccg aagtcgtagc gtgtaaacgg caatttctga tcgtgtttcg
    19861 gtagtgtgtt gtgctgcata aagtcctgtc cagaaagaag tcggcctcgc aaaaatgaat
    19921 aaacccagtt tctcttgcgc aacaggtgga gaagtcgaat acgcagtatg taaaaaatga
    19981 ggcaacagat tcactgggga ggtcgcgagg aggcgcaatt ctacaaaatt gcgcatttca
    20041 ctactagtct ggagtcggcc cggatatgag ctgcaactat tgcaaataaa gaaattaagc
    20101 tggaaggcat cacgcaatga ggaagattgc cgggtgctga cggcaaaaag caatcgtaaa
    20161 atcagcggca agccggaa
//
LOCUS       DADSYM010000088        19700 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2415
            SAMN08039579-rid11723173.denovo.088, whole genome shotgun sequence.
ACCESSION   DADSYM010000088 DADSYM010000000
VERSION     DADSYM010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039579
            Sequence Read Archive: SRR6362533
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 19700)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19700)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980162.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 03:39:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,484
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 4,347
            CDSs (with protein)               :: 4,347
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 43 of 85
            Pseudo Genes (incomplete)         :: 37 of 85
            Pseudo Genes (internal stop)      :: 19 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19700
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039579-rid11723173.denovo.088"
                     /strain="LGE2415"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8302 N 95.9628 W"
                     /collection_date="2015-08-24"
     gene            119..334
                     /gene="bdm"
                     /locus_tag="J1Y83_002723"
     CDS             119..334
                     /gene="bdm"
                     /locus_tag="J1Y83_002723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310112.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm-dependent modulation protein"
                     /protein_id="HBA7630858.1"
                     /translation="MFTYYQAENSTAEPALVNAIEQGLRAEHGVVTEDDILMELTKWV
                     EASDNDILSDIYQQTINYVVSGQHPTL"
     gene            436..573
                     /gene="sra"
                     /locus_tag="J1Y83_002724"
     CDS             436..573
                     /gene="sra"
                     /locus_tag="J1Y83_002724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stationary-phase-induced ribosome-associated
                     protein"
                     /protein_id="HBA7630859.1"
                     /translation="MKSNRQARHILGLDHKISNQRKIVTEGDKSSVVNNPTGRKRPAE
                     K"
     gene            730..2427
                     /locus_tag="J1Y83_002725"
     CDS             730..2427
                     /locus_tag="J1Y83_002725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent malic enzyme"
                     /protein_id="HBA7630860.1"
                     /translation="MEPKTKKQRSLYIPYAGPVLLEFPLLNKGSAFSMEERRNFNLLG
                     LLPEVVETIEEQAERAWIQYQGFKTEIDKHIYLRNIQDTNETLFYRLVNNHLDEMMPV
                     IYTPTVGAACERFSEIYRRSRGVFISYQNRHNMDDILQNVPNHNIKVIVVTDGERILG
                     LGDQGIGGMGIPIGKLSLYTACGGISPAYTLPVVLDVGTNNQQLLNDPLYMGWRNPRI
                     TDDEYYEFVDEFIQAVKQRWPDVLLQFEDFAQKNAMPLLNRYRNEICSFNDDIQGTAA
                     VTVGTLIAASRAAGGQLSEKKIVFLGAGSAGCGIAEMIIAQTQREGLSEEAARQKVFM
                     VDRFGLLTDKMPNLLPFQTKLVQKRENLSDWDTDSDVLSLLDVVRNVKPDILIGVSGQ
                     TGLFTEEIIREMHKHCPRPIVMPLSNPTSRVEATPQDIIAWTEGNALVATGSPFNPVV
                     WKDKIYPIAQCNNAFIFPGIGLGVIASGASRITDEMLMSASETLAQYSPLVLNGEGLV
                     LPELKDIQKVSRAIAFAVGKMAQQQGVAVKTSAEALQQAIDDNFWHAEYRDYRRTSI"
     gene            2561..3571
                     /gene="adhP"
                     /locus_tag="J1Y83_002726"
     CDS             2561..3571
                     /gene="adhP"
                     /locus_tag="J1Y83_002726"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase AdhP"
                     /protein_id="HBA7630861.1"
                     /translation="MKAAVVTKDHHVDVTDKTLRSLKHGEALLKMECCGVCHTDLHVK
                     NGDFGDKTGVILGHEGIGVVAEVGPGVTSLKPGDRASVAWFYEGCGHCEYCNSGNETL
                     CRSVKNAGYSVDGGMAEECIVVADYAVKVPDGLDSAAASSITCAGVTTYKAVKLSKIR
                     PGQWIAIYGLGGLGNLALQYAKNVFNAKVIAIDVNDEQLKLATEMGADLAINSRTEDA
                     ARIVQEKAGGAHAAVVTAVAKAAFNSAVDAVRAGGRVVAVGLPPESMSLDIPRLVLDG
                     IEVVGSLVGTRQDLTEAFQFAAEGKVVPKVALRPLADINTIFTEMEEGKIRGRMVIDF
                     RR"
     gene            3757..4035
                     /locus_tag="J1Y83_002727"
     CDS             3757..4035
                     /locus_tag="J1Y83_002727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003833016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Killer protein"
                     /protein_id="HBA7630862.1"
                     /translation="MIMNFRHKGLRDLFLLGKTSGVIPTQVKRLRHRLAVIDAACCLA
                     DIDMPGYRLHPLSGDRDGIWAISVSGNWRITFEFVNGDAYILDYEDYH"
     gene            4035..4319
                     /locus_tag="J1Y83_002728"
     CDS             4035..4319
                     /locus_tag="J1Y83_002728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415994.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antidote protein"
                     /protein_id="HBA7630863.1"
                     /translation="MKMANHPRPGDIIQESLDELNVSLREFARAMEIAPSTASRLLTG
                     KAALTPEMAIKLSVVIGSSPQMWLNLQNAWSLAEAEKTVDVSRLRRLVTQ"
     gene            complement(4548..5201)
                     /locus_tag="J1Y83_002729"
     CDS             complement(4548..5201)
                     /locus_tag="J1Y83_002729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase cytochrome b556 subunit"
                     /protein_id="HBA7630864.1"
                     /translation="MSKSKMIVRTKFIDRACHWTVVICFFLVALSGISFFFPTLQWLT
                     QTFGTPQMGRILHPFFGIAIFVALMFMFVRFVHHNIPDKKDIPWLLNIVEVLKGNEHK
                     VADVGKYNAGQKMMFWSIMSMIFVLLVTGVIIWRPYFAQYFPIQVVRYSLLIHAAAGI
                     ILIHAILIHMYMAFWVKGSIKGMIEGKVSRRWAKKHHPRWYREIEKAEAKKESEEGL"
     gene            complement(5194..6078)
                     /gene="fdxH"
                     /locus_tag="J1Y83_002730"
     CDS             complement(5194..6078)
                     /gene="fdxH"
                     /locus_tag="J1Y83_002730"
                     /EC_number="1.17.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase subunit beta"
                     /protein_id="HBA7630865.1"
                     /translation="MAMETQDIIKRSATNSITPPSQVRDYKAEVAKLIDVSTCIGCKA
                     CQVACSEWNDIRDEVGHCVGVYDNPADLSAKSWTVMRFSETEQNGKLEWLIRKDGCMH
                     CEDPGCLKACPSAGAIIQYANGIVDFQSENCIGCGYCIAGCPFNIPRLNKEDNRVYKC
                     TLCVDRVSVGQEPACVKTCPTGAIHFGTKKEMLELAEQRVAKLKARGYEHAGVYNPEG
                     VGGTHVMYVLHHADQPELYHGLPKDPKIDTSVSLWKGALKPLAAAGFIATFAGLIFHY
                     IGIGPNKEVDDDEEGNHE"
     gene            complement(6091..9138)
                     /gene="fdnG"
                     /locus_tag="J1Y83_002731"
     CDS             complement(6091..9138)
                     /gene="fdnG"
                     /locus_tag="J1Y83_002731"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:complement(8551..8553),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit alpha"
                     /protein_id="HBA7630866.1"
                     /translation="MDVSRRQFFKICAGGMAGTTVAALGFAPKQALAQARNYKLLRAK
                     EIRNTCTYCSVGCGLLMYSLGDGAKNAREAIYHIEGDPDHPVSRGALCPKGAGLLDYV
                     NSENRLRYPEYRAPGSDKWQRISWEEAFSRIAKLMKADRDANFIEKNEQGVTVNRWLS
                     TGMLCASGASNETGMLTQKFARSLGMLAVDNQARVUHGPTVASLAPTFGRGAMTNHWV
                     DIKNANVVMVMGGNAAEAHPVGFRWAMEAKNNNDATLIVVDPRFTRTASVADIYAPIR
                     SGTDITFLSGVLRYLIENNKINAEYVKHYTNASLLVRDDFAFEDGLFSGYDAEKRQYD
                     KSSWNYQFDENGYAKRDDTLTHPRCVWNLLKAHVSRYTPDVVENICGTPKADFLKVCE
                     VLASTSAPDRTTTFLYALGWTQHTVGAQNIRTMAMIQLLLGNMGMAGGGVNALRGHSN
                     IQGLTDLGLLSTSLPGYLTLPSEKQVDLQSYLEANTPKSTLADQVNYWSNYPKFFVSL
                     MKSFYGDAAQKENNWGYDWLPKWDQTYDVIKYFNMMDEGKVTGYFCQGFNPVASFPDK
                     NKVVSCLSKLKYMVVIDPLVTETSTFWQNHGESNDVDPASIQTEVFRLPSTCFAEEDG
                     SIANSGRWLQWHWKGQDAPGEARNDGEILAGIYHHLRELYQAEGGKGVEPLMKMSWNY
                     KQPHEPQSDEVAKENNGYALEDLYDANGVLIAKKGQLLSSFAHLRDDGTTASSCWIYT
                     GSWTEQGNQMANRDNSDPSGLGNTLGWAWAWPLNRRVLYNRASADINGKPWDPKRMLI
                     QWNGSKWTGNDIPDFGNAAPGTPTGPFIMQPEGMGRLFAINKMAEGPFPEHYEPIETP
                     LGTNPLHPNVVSNPVVRLYEQDALRMGKKEQFPYVGTTYRLTEHFHTWTKHALLNAIA
                     QPEQFVEISETLAEAKGINNGDRVTVSSKRGFIRAVAVVTRRLKPLNVNGQQVETVGI
                     PIHWGFEGVARKGYIANTLTPNVGDANSQTPEYKAFLVNIEKA"
     gene            9370..10251
                     /gene="yddG"
                     /locus_tag="J1Y83_002732"
     CDS             9370..10251
                     /gene="yddG"
                     /locus_tag="J1Y83_002732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415990.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid efflux DMT transporter YddG"
                     /protein_id="HBA7630867.1"
                     /translation="MTRQKATLIGLIAIVLWSTMVGLIRGVSEGLGPVGGAAAIYSLS
                     GLLLIFTVGFPRIRQIPKGYLLAGSLLFVSYEICLALSLGYAATRHQAIEVGMVNYLW
                     PSLTILFAILFNGQKTNWLIVPGLLLALVGVCWVLGGDNGLHYDQIINNITTSPLSYF
                     LAFIGAFIWAAYCTVTNKYARGFNGITVFVLLTGASLWVYYFLTPQPEMVFSTPVMIK
                     LISAAFTLGFAYAAWNVGILHGNVTIMAVGSYFTPVLSSALAAVLLSAPLSFSFWQGA
                     LMVCGGSLLCWLATRRG"
     gene            10524..11912
                     /locus_tag="J1Y83_002733"
     CDS             10524..11912
                     /locus_tag="J1Y83_002733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NarK family nitrate/nitrite MFS transporter"
                     /protein_id="HBA7630868.1"
                     /translation="MALQNEKNSRYLLRDWKPENPAFWENKGKHIARRNLWISVSCLL
                     LAFCVWMLFSAVTVNLNKIGFNFTTDQLFLLTALPSVSGALLRVPYSFMVPIFGGRRW
                     TVFSTAILIIPCVWLGIAVQNPNTPFGIFIVIALLCGFAGANFASSMGNISFFFPKAK
                     QGSALGINGGLGNLGVSVMQLVAPLVIFVPVFAFLGVNGVPQADGSVMSLANAAWIWV
                     PLLAIATIAAWSGMNDIASSRASIADQLPVLQRLHLWLLSLLYLATFGSFIGFSAGFA
                     MLAKTQFPDVNILRLAFFGPFIGAIARSVGGAISDKFGGVRVTLINFIFMAIFSALLF
                     LTLPGTGSGNFIAFYAVFMGLFLTAGLGSGSTFQMIAVIFRQITIYRVKMKGGSDEQA
                     QREAVTETAAALGFISAIGAVGGFFIPQAFGMSLNMTGSPVGAMKVFLIFYIVCVLLT
                     WLVYGRRKFSQK"
     gene            12007..15747
                     /locus_tag="J1Y83_002734"
     CDS             12007..15747
                     /locus_tag="J1Y83_002734"
                     /EC_number="1.7.99.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit alpha"
                     /protein_id="HBA7630869.1"
                     /translation="MSKLLDRFRYFKQKGETFADGHGQVMHSNRDWEDSYRQRWQFDK
                     IVRSTHGVNCTGSCSWKIYVKNGLVTWEIQQTDYPRTRPDLPNHEPRGCPRGASYSWY
                     LYSANRLKYPLIRKRLIELWREALKQHSDPVLAWASIMNDPQKCLSYKQVRGRGGFIR
                     SNWQELNQLIAAANVWTIKTYGPDRVAGFSPIPAMSMISYAAGTRYLSLLGGTCLSFY
                     DWYCDLPPASPMTWGEQTDVPESADWYNSSYIIAWGSNVPQTRTPDAHFFTEVRYKGT
                     KTIAITPDYSEVAKLCDQWLAPKQGTDSALAMAMGHVILKEFHLDNPSDYFINYCRRY
                     SDMPMLVMLEPRDDGSYVPGRMVRASDLVDGLGESNNPQWKTVAVNTAGELVVPNGSI
                     GFRWGEKGKWNLESIAAGTETELSLTLLGQHDAVAGVAFPYFGGIENPHFRSVKHNPV
                     LVRQLPVKNLTLADGNTCPVVSVYDLVLANYGLDRGLEDENSAKDYAEIKPYTPAWGE
                     QITGVPRQYIETIAREFADTAHKTHGRSMIILGAGVNHWYHMDMNYRGMINMLIFCGC
                     VGQSGGGWAHYVGQEKLRPQTGWLPLAFALDWNRPPRQMNSTSFFYNHSSQWRYEKVS
                     AQELLSPLADASKYSGHLIDFNVRAERMGWLPSAPQLGRNPLGIKAEADKAGLSPTEF
                     TAQALKSGDLRMACEQPDSGSNHPRNLFVWRSNLLGSSGKGHEYMQKYLLGTESGIQG
                     EELGPTEGIQPEEVEWQTAAIEGKLDLLVTLDFRMSSTCLFSDIVLPTATWYEKDDMN
                     TSDMHPFIHPLSAAVDPAWESRSDWEIYKGIAKAFSQVCVGHLGKETDVVLQPLLHDS
                     PAELSQPCEVLDWRKGECDLIPGKTAPNIVAVERDYPATYERFTSLGPLMDKLGNGGK
                     GISWNTQDEIDFLGKLNYTKRDGPAQGRPLIDTAIDASEVILALAPETNGHVAVKAWQ
                     ALGEITGREHTHLALHKEDEKIRFRDIQAQPRKIISSPTWSGLESDHVSYNAGYTNVH
                     ELIPWRTLSGRQQLYQDHPWMRAFGESLVAYRPPIDTRSVSEMRQIPPNGFPEKALNF
                     LTPHQKWGIHSTYSENLLMLTLSRGGPIVWISETDARELTIVDNDWVEVFNANGALTA
                     RAVVSQRVPPGMTMMYHAQERIMNIPSSEVTGMRGGIHNSVTRVCPKPTHMIGGYAQL
                     AWGFNYYGTVGSNRDEFIMIRKMKNVNWLDDEGRDQVQEAKK"
     gene            15744..17288
                     /gene="narH"
                     /locus_tag="J1Y83_002735"
     CDS             15744..17288
                     /gene="narH"
                     /locus_tag="J1Y83_002735"
                     /EC_number="1.7.99.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit beta"
                     /protein_id="HBA7630870.1"
                     /translation="MKIRSQVGMVLNLDKCIGCHTCSVTCKNVWTGREGMEYAWFNNV
                     ETKPGIGYPKNWEDQDEWQGGWVRDVNGKIRPRLGSKMGVITKIFANPVVPQIDDYYE
                     PFTFDYEHLHSAPEGKHIPTARPRSLIDGKRMDKVIWGPNWEELLGGEFEKRARDRNF
                     EAMQKEMYGQFENTFMMYLPRLCEHCLNPSCVATCPSGAIYKREEDGIVLIDQDKCRG
                     WRLCISGCPYKKIYFNWKSGKSEKCIFCYPRIESGQPTVCSETCVGRIRYLGVLLYDA
                     DRIEEAASTEREVDLYERQCEVFLDPHDPSVIEEALKQGIPQNVIDAAQRSPVYKMAM
                     DWKLALPLHPEYRTLPMVWYVPPLSPIQSYADAGGLPKSEGVLPAIESLRIPVQYLAN
                     MLSAGDTGPVLRALKRMMAMRHYMRSQTVEGVTDTRAIDEVGLSVAQVEEMYRYLAIA
                     NYEDRFVIPTSHREMAGDAFAERNGCGFTFGDGCHGSDSKFNLFNSSRIDAINITEVR
                     DKAEGE"
     gene            17288..17983
                     /gene="narW"
                     /locus_tag="J1Y83_002736"
     CDS             17288..17983
                     /gene="narW"
                     /locus_tag="J1Y83_002736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase molybdenum cofactor assembly
                     chaperone"
                     /protein_id="HBA7630871.1"
                     /translation="MQILKVIGLLMEYPDELLWECKEDALALIRRDAPILTDFTRNLL
                     NAPLLDKQAEWCEVFDRGRTTSLLLFEHVHAESRDRGQAMVDLLAEYEKVGLQLNCRE
                     LPDYLPLYLEYLSVLPDDQAKEGLLNVAPILALLGGRLKQREAPWYALFDALLQLAGS
                     PLSSDSVTKQIRSEERDDTRQALDAVWEEEQVKFIEDNATACDSSPLNQYQRRFSQDV
                     APQYVDISAGGGK"
     gene            17983..18660
                     /gene="narI"
                     /locus_tag="J1Y83_002737"
     CDS             17983..18660
                     /gene="narI"
                     /locus_tag="J1Y83_002737"
                     /EC_number="1.7.99.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="respiratory nitrate reductase subunit gamma"
                     /protein_id="HBA7630872.1"
                     /translation="MQYLNVFFYDIYPYLCGTVFILGSWLRYDYGQYTWRAASSQMLD
                     KRGMVLWSNLFHIGILGIFFGHLFGMLTPHWMYAWFLPVAVKQQMAMILGGICGVLTL
                     IGGAGLLVRRLTNPRIRVTSSGADILILAILLIQCILGLTTIPFSAQHPDGSEMMKLV
                     GWAQSIVTFQGGASTHLDGVAFIFRVHLVLGMTIFLLFPFTRLVHVWSAPFEYFTRRY
                     QIVRSRR"
     gene            18740..19633
                     /locus_tag="J1Y83_002738"
     CDS             18740..19633
                     /locus_tag="J1Y83_002738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family isomerase"
                     /protein_id="HBA7630873.1"
                     /translation="MKPQVYHVDAFTSQPFRGNSAGVVFPADNLSEAQMQLIARELGH
                     SETAFLLHSDDSDVRIRYFTPTVEVPICGHATVAAHYVRAKVLGLGNCTVWQTSLAGK
                     HRVTIEKQNDDYRISLEQGTPGFEPPLEGETRAAIINALHLTEDDILPGLPIQVATTG
                     HSKVMIPLKPEVDIDALSPDLAALTAISKQIGCNGFFPFQIRPGKNETDGRMFSPAIG
                     IVEDPVTGNANGPMGAWLVHHNVLPHDGNVLRVKGHQGRALGRDGVIEVTVTIRDNQP
                     EKVTISGAAVILFHAEWAIEL"
BASE COUNT         4669 a         5139 c         5109 g         4783 t
ORIGIN      
        1 aatcatcaac caggactaat cttaacaacg aagcgtcaaa tatttgccat gctgaatgtg
       61 cgttataaat ctgcgatccg tagcagacac cataaataca cagacacgga gaatcactat
      121 gtttacttat tatcaggcag aaaattcaac agcagaaccc gctctggtta atgccattga
      181 gcaaggcctt cgggcagagc acggtgttgt cactgaagat gacattctca tggagctgac
      241 caaatgggtg gaagcttctg ataacgacat cctcagtgat atctaccagc aaacgatcaa
      301 ttatgtggtc agtggtcagc accctacgct ttaaggtgct atgcttgatc ggcaacctaa
      361 tttaggggtt tagcacgtgt ttcttcgcta cggcgatgtt gtccttaaaa ctagcaacag
      421 gattgaggag ttaaaatgaa atcgaaccgt caggcacgtc atattcttgg actggaccat
      481 aaaatttcta accagcgcaa aatagttacc gaaggtgaca aatccagcgt ggtaaataat
      541 ccaaccggca gaaaacgccc cgctgaaaag taattcataa ccatcagtcc tcaatgacga
      601 ttaaacacca ttgcctgcgc aatggtgttt ttgtttttat ctgctttata cttggtactt
      661 gacgccctgg cggtaatgca aagacgataa aagcccccag ggatggatat tcaaaaaaga
      721 gtgagtgaca tggaaccaaa aacaaaaaaa cagcgttcgc tttatatccc ttacgctggc
      781 cctgtattgc tggaatttcc gttgttgaat aaaggcagcg ccttcagcat ggaagaacgc
      841 cgtaacttca acctgctggg gttactgccg gaagtggtcg aaaccatcga agaacaagcg
      901 gaacgagcat ggatccagta tcagggattc aaaaccgaaa tcgacaaaca catctacctg
      961 cgtaacatcc aggacactaa cgaaaccctc ttctaccgtc tggtaaacaa tcatcttgat
     1021 gagatgatgc cagtcattta caccccaacc gtcggcgcag cctgtgagcg tttttctgag
     1081 atctaccgcc gttcacgcgg tgtgtttatc tcttaccaga accgccacaa tatggacgat
     1141 attctgcaaa acgtaccaaa ccataatatc aaagtgattg tggtgactga cggcgaacgt
     1201 attctggggc ttggtgacca gggcatcggc gggatgggca ttccgatcgg taaactgtcg
     1261 ctctataccg cctgtggcgg catcagtccg gcctataccc ttccggtggt gctggatgtc
     1321 ggcaccaaca accaacagct acttaacgat ccgctgtata tgggctggcg taatccgcgt
     1381 atcactgacg acgaatacta tgaattcgtt gatgaattta tccaggctgt gaaacaacgc
     1441 tggccggacg tactgttgca gtttgaagac ttcgcacaaa aaaatgcgat gccgttactt
     1501 aaccgctatc gcaatgaaat ttgttctttt aacgatgaca ttcagggcac cgcggcggta
     1561 acagtcggca cactgatcgc agccagccgc gcggcaggtg gtcagttaag cgagaaaaaa
     1621 atcgtcttcc ttggcgcagg ttcagcggga tgcggcattg ccgaaatgat catcgcccag
     1681 acccagcgcg aaggattaag cgaggaagcg gcgcggcaga aagtctttat ggtcgatcgc
     1741 tttggcctgc tgaccgacaa gatgccgaac ctgctgcctt tccagaccaa actggtgcag
     1801 aagcgcgaaa atctcagtga ctgggatacc gacagcgatg tgttgtcact gctggatgtg
     1861 gtgcgtaatg taaaaccaga tattctgatt ggcgtctcag gacagaccgg gctgtttacg
     1921 gaagagatca tccgtgagat gcataaacac tgtccgcgtc cgatcgtgat gccgctgtct
     1981 aacccgactt cacgcgtgga agccacacca caggacatta tcgcctggac tgaaggtaac
     2041 gcgctggtcg ccactggcag tccgtttaat ccagtggtat ggaaagataa aatctaccct
     2101 atagcccagt gcaacaacgc ctttattttt ccgggcatcg ggctgggtgt tattgcttcc
     2161 ggcgcgtcac gtatcaccga tgagatgctg atgtcggcaa gtgaaacgct tgctcagtat
     2221 tcgccgctgg tcctgaacgg cgaaggtctg gtactaccgg aactgaaaga tattcagaaa
     2281 gtctcccgcg caattgcctt tgcggttggc aaaatggcgc agcagcaagg cgtggcggtg
     2341 aaaacgtctg ccgaagctct gcaacaggcc attgatgata atttctggca cgccgaatac
     2401 cgcgactacc gccgtacctc catctaagcc tgcgcccggt agtgaaggct accgggcaat
     2461 tttcctctcc ctttttcaga tctcatccat actgggtagt ggcgaataaa tctcatttgc
     2521 ctcacctgct atgcagaaca tcatccgaaa aggaggaact atgaaggctg cagttgttac
     2581 gaaggatcat catgttgacg ttacggataa aacactgcgc tcactgaaac atggcgaagc
     2641 cctgctgaaa atggagtgtt gtggtgtatg tcataccgat cttcatgtta agaatggcga
     2701 ttttggtgac aaaaccggcg tgattctggg acacgaaggt attggtgtgg tggcagaagt
     2761 tggccctggt gttacttcat taaaaccagg cgatcgtgcc agcgtggcgt ggttctacga
     2821 aggatgcggt cattgcgaat actgtaacag tggtaacgaa acgctctgcc gttcagttaa
     2881 aaatgccgga tacagcgttg atggcgggat ggcggaagag tgtatcgtgg tcgccgatta
     2941 cgcggtaaaa gtgccagatg gtctggactc ggcggcggcc agcagcatta cctgtgcggg
     3001 ggtcactacc tacaaagccg ttaagctgtc aaaaattcgt ccaggacagt ggattgctat
     3061 ctacggtctt ggcggtctgg gtaacctcgc cctgcaatac gcgaagaatg tctttaacgc
     3121 caaagtgatc gccattgatg tcaatgatga acagttaaaa ctggcaaccg aaatgggtgc
     3181 agatttagcg attaactcac gcaccgaaga cgccgccaga attgtgcagg agaaagccgg
     3241 tggcgctcac gcagcggtgg tgacagcggt agctaaagct gcgtttaact cggcggtgga
     3301 tgctgtccgt gcaggtggtc gtgtcgtggc tgtcggtctg ccgccggagt ctatgagcct
     3361 ggacatccca cggctggtgc tggatggtat tgaagtggtc ggttcgctgg tcggcacgcg
     3421 tcaggatcta accgaagcct tccagtttgc cgccgaaggc aaagtggtgc cgaaagtcgc
     3481 cctgcgtccg ttagcggaca tcaacaccat ctttaccgag atggaagaag gcaaaatccg
     3541 tggccgtatg gtgattgatt tccgtcgcta agaggtcttt gctgcgactg ccatgttcgg
     3601 gtcgcagcct gttttgcgag acgctttccg caatttcact cttcttcttt tcctccccca
     3661 tctttcccct cacaattcgc gaaaacgtag cgaaatttgt tgcgctgcac ggaacaatag
     3721 tgatagtgtt cctttcgagg gaacaaggat gaagagatga taatgaattt cagacacaag
     3781 ggattgcgtg acttgtttct tctcggcaaa acctccggcg ttataccgac gcaagtcaaa
     3841 cgattacgcc accgactcgc tgtgattgat gcagcatgct gtcttgctga tatcgatatg
     3901 cccggttacc gactacatcc gttaagcggc gatcgcgatg gaatttgggc gatatctgtc
     3961 tcgggtaact ggcgaatcac atttgaattc gtcaatggcg atgcatacat actggattac
     4021 gaggactatc actgatgaaa atggccaatc atccccgccc gggggacatt attcaggaat
     4081 cactggacga acttaatgtc agcctgcgcg agtttgccag agcaatggaa attgcgccct
     4141 caacggcaag ccgattgctg accggaaaag cagctttgac gccagaaatg gcaataaaac
     4201 tttccgtggt aatcggcagt tcgccgcaaa tgtggctgaa tctgcaaaat gcctggagtc
     4261 tggcagaggc agaaaaaaca gtggatgtgt cgcgactgcg ccgtctggtt acgcaataaa
     4321 aaaatggcac cttataaagc gccatttgaa actgatgaca aacgcaacat tgcccgatgc
     4381 gctacgctta tcaggcctac acgttcactg caatctcctg gattttaacg gtttgtaggc
     4441 cggataaggc gttcacgccg catccggcat gaacaaagtg cacgttgtct acaataaaaa
     4501 aatggcgcct tttacagcgc cattttcagc ttaaagctca aaagagatta taacccttct
     4561 tcactctctt ttttcgcttc tgccttctcg atttcacgat accagcgcgg atggtgtttc
     4621 ttcgcccagc gacggctcac cttcccttcg atcatccctt taatcgatcc tttcacccag
     4681 aatgccatat acatatggat caggatggcg tggatcagga tgatacccgc agccgcatgg
     4741 atcagcaggc tataacggac aacctgtatc gggaagtact gtgcaaagta cggacgccag
     4801 ataatcactc cggtcaccag cagcacgaaa atcatgctca tgattgacca gaacatcatc
     4861 ttttgcccgg cgttgtactt accgacatcc gccactttgt gctcattgcc ttttaatact
     4921 tcgacaatgt tcaacagcca cggaatatct ttcttatccg ggatgttgtg atgcacaaaa
     4981 cgcacaaaca taaacatcag cgcgacgaaa atcgcaatgc cgaagaacgg gtgcaaaatg
     5041 cgccccatct gcggcgtacc gaaggtttgc gtcagccatt gcagcgtcgg gaagaagaac
     5101 gaaatcccgg acagcgccac caggaagaag caaatcacca cggtccagtg acaggcgcga
     5161 tcaataaatt tggtgcgcac aatcattttc gacttactca tgatttccct cctcgtcatc
     5221 gtccacttcc ttattcgggc caataccgat gtagtggaaa atcaacccgg caaaagtggc
     5281 aataaagcca gccgctgcca gtggtttcaa cgcgcctttc cacaggctta ccgaggtatc
     5341 gatcttcgga tctttcggca gaccatgata cagctccggc tgatcggcat gatgcagcac
     5401 gtacataacg tgcgtaccac cgaccccttc cgggttgtag acgccagcat gttcgtaacc
     5461 acgcgctttc agtttcgcta cgcgctgttc cgccagctcc agcatctcct tcttggtgcc
     5521 gaagtggata gccccggtcg gacaggtttt cacacaagcc ggttcctggc cgacgctgac
     5581 gcgatcgacg cagagcgtgc atttatatac ccggttatcc tctttgttga ggcgcggaat
     5641 attaaacgga cacccggcaa tgcagtaacc acagccgatg cagttttccg actggaaatc
     5701 gacaatcccg ttagcgtact gaatgattgc accagcagac gggcacgcct tcaggcagcc
     5761 cggatcttca cagtgcatac agccatcttt acggatcagc cactccagct tgccgttctg
     5821 ttcggtttcg ctaaagcgca tcaccgtcca ggacttggcg ctcagatcgg cggggttatc
     5881 gtaaaccccg acgcagtgcc ccacttcatc acggatgtcg ttccactccg aacacgccac
     5941 ctgacaggct ttacagccga tacaggtgga aacgtcgata agtttcgcga cttctgcttt
     6001 gtaatcacgc acctgagaag gcggcgtgat ggagttagtt gcggaccttt tgataatgtc
     6061 ctgcgtttcc atagccatct gttcgccccc ttacgccttc tcgatgttga ctaagaacgc
     6121 tttatattcc ggcgtttgcg agtttgcatc accgacattc ggcgtcagag tgttagcgat
     6181 ataaccttta cgcgcgacac cctcaaagcc ccagtggatt ggaataccca ccgtttcaac
     6241 ctgctgaccg tttacattca gcggtttcag acggcgcgtt accacagcca ccgcgcggat
     6301 aaagccacgc ttgctggaga cagtgacacg atcgccatta ttaatgcctt tcgcctccgc
     6361 cagcgtttcg ctgatttcca caaactgttc cggctgagca attgcgttga gcaatgcgtg
     6421 cttggtccag gtgtggaagt gctccgtcag acgataggtc gtacccacat acgggaactg
     6481 ctctttttta cccatccgca gcgcgtcttg ttcatacaga cgaacaaccg ggttagacac
     6541 cacgttcgga tgcaacgggt tagtgcccag cggcgtttca attggctcgt agtgttccgg
     6601 gaacggacct tccgccattt tgttgatggc aaacaggcgt cccatccctt ccggctgcat
     6661 gataaacggc ccggttggcg tacccggtgc ggcattgccg aagtccggaa tatcgttacc
     6721 cgtccacttg ctaccgttcc actggatcag catccgtttc ggatcccacg gtttaccgtt
     6781 gatatccgcc gaagcacggt tgtagagtac gcgacggttg agcggccacg cccaggccca
     6841 tcccagcgta ttccccagac cggacgggtc ggagttatcg cggttagcca tctggttgcc
     6901 ctgctctgtc cagctaccgg tgtagatcca gcaagacgat gcggttgtac cgtcatcacg
     6961 cagatgcgca aagctactca gcaactgacc tttcttcgca atcagcacgc cattagcgtc
     7021 atagagatct tccagcgcat agccgttgtt ctctttcgcc acttcgtcag actgcggttc
     7081 gtgcggctgc ttgtagttcc agctcatctt catcagcggt tctacgcctt taccaccctc
     7141 ggcctggtac agctcgcgca gatgatggta gatacccgcc agaatttcgc cgtcgttacg
     7201 tgcttcgccc ggcgcgtcct gacctttcca gtgccactgc aaccagcgac cggagttagc
     7261 gatagaacca tcttcttcag caaagcaggt cgaaggcaga cggaatactt cagtctgaat
     7321 agatgccgga tcgacatcgt tcgactcgcc gtggttctgc cagaaggtag aggtttcagt
     7381 caccagcgga tcgataacca ccatgtactt cagcttgctc aggcagctca ccactttgtt
     7441 tttgtccggg aaggacgcaa ccgggttaaa gccctggcag aaataaccgg tgactttacc
     7501 ttcatccatc atgttgaaat acttgatgac gtcgtaggtt tggtcccact tcggcagcca
     7561 gtcgtagccc cagttgttct ctttctgcgc ggcatcgccg tagaaagatt tcatcaggct
     7621 aacgaaaaac ttcggatagt tgctccagta gttcacctga tcagccagcg tcgatttcgg
     7681 cgtgttcgct tccagatacg actgcaaatc aacctgtttt tctgacggca gcgtcagata
     7741 acctggcagg ctggtagaga gcaggcctaa gtcagtcagg ccctgaatgt tggagtgacc
     7801 acgcaatgcg ttcacgccgc caccggccat acccatgtta ccgagcagca actggatcat
     7861 cgccatcgta cggatgttct gcgcacccac ggtgtgctgc gtccagccca gcgcgtacag
     7921 gaaggtggtt gtgcgatccg gtgcgctggt ggaggccagc acttcacaca ctttcaggaa
     7981 gtcggctttt ggcgtaccgc aaatgttttc tactacgtcc ggcgtgtagc gggaaacgtg
     8041 cgctttcagc aggttccata cacagcgcgg atgagtcagc gtgtcatcgc gtttcgcata
     8101 gccgttttca tcgaactgat agttccagga cgacttatcg tattgacgtt tttcagcgtc
     8161 gtagccgctg aacagaccgt cttcgaaagc aaaatcatca cgcaccagca ggctggcgtt
     8221 ggtgtaatgc ttaacgtatt cggcgttgat tttgttgttt tcgatcaggt agcgcaaaac
     8281 gccagacagg aacgtaatgt ccgtaccgga acgaataggt gcgtaaatat ccgccacaga
     8341 agcggtacgc gtaaaacggg gatcgacaac gatcaaggtt gcatcgttgt tgtttttcgc
     8401 ttccatcgcc cagcggaaac cgacgggatg cgcttcagca gcgttaccgc ccatcaccat
     8461 tacgacgtta gcgtttttga tatccaccca gtggttggtc atcgcaccgc gaccaaatgt
     8521 tggagcaaga cttgctaccg ttggtccgtg tcagacgcgc gcctggttgt ctaccgccag
     8581 catcccgagg gagcgggcaa atttttgagt cagcatcccg gtttcgttgc tggcaccgga
     8641 agcacacagc ataccggtag aaagccaacg gtttaccgtt acgccctgct cgttcttttc
     8701 aataaagtta gcgtcacggt cggctttcat cagcttcgca atacgggaga atgcttcttc
     8761 ccagctaatg cgctgccatt tgtcagaacc tggcgcacga tattccgggt agcgcagacg
     8821 gttttcgctg ttgacgtaat ccagcaaacc ggcccctttc gggcacagcg caccacggct
     8881 taccggatga tccgggtcac cttcaatgtg ataaatcgct tctctggcgt ttttcgcgcc
     8941 atcacccagg ctatacatca atagcccgca acctacggaa cagtatgtgc aggtgttacg
     9001 gatctctttc gcgcgtaata atttgtagtt tcgcgcctga gccagtgctt gcttcggggc
     9061 aaagcccaat gctgctactg ttgttcccgc cataccgccc gcgcagattt taaaaaattg
     9121 tctgcgactg acgtccattg ctttcctctt ttttcagggg tattactgcg cgaggaaact
     9181 aacagtaaaa ttctgaatct ttttgcgcga aatcaaaaag aacacgtgtt tgccacaaaa
     9241 taatccgcct gcgcaaattg acctacctca atagcggtag aaaaacgcac cactgcctga
     9301 caggccagtt aaaaaaatgc tataaaattc agcttaattt ttaacggtaa gagagacaaa
     9361 acagcgagca tgacacgaca aaaagcaacg ctcatagggc taatagcgat cgtcctgtgg
     9421 agcacgatgg taggattgat tcgcggtgtc agtgaggggc tcggcccggt cggcggcgca
     9481 gctgctatct attcattaag cgggctgctg ttaatcttca cggttggatt tccgcgtatt
     9541 cggcagatcc cgaaaggcta tttactcgcc gggagtctgt tattcgtcag ctatgaaatc
     9601 tgtctggcgc tttccttagg gtatgcggcg acccgtcatc aggcgattga agtggggatg
     9661 gtgaactatc tatggcccag cctgacaatt ctctttgcca ttctgtttaa tggtcagaaa
     9721 accaactggc tgattgtacc aggattatta ttagccctgg tcggcgtctg ctgggtgtta
     9781 ggcggtgaca atgggttaca ttatgatcaa attattaata atatcaccac cagcccattg
     9841 agttatttcc tggcgttcat tggggcgttt atctgggcag cctactgcac agtaacgaat
     9901 aaatacgcac gcggatttaa cggaattacc gtttttgtcc tgctaacggg agcaagtctg
     9961 tgggtttact attttcttac gccacaacca gaaatggtat ttagcacgcc cgtcatgatt
    10021 aaactcatct ctgcggcatt taccttagga tttgcttatg ctgcatggaa tgtcggtata
    10081 ttacatggca acgtcaccat aatggcggta ggttcgtatt ttacgcccgt cctttcctca
    10141 gcgcttgcag ccgtgctgct cagcgccccg ctgtcgttct cgttctggca aggcgcgcta
    10201 atggtctgcg gcggttccct gctctgctgg ctggcgacac gtcgtggcta aaataattgt
    10261 cgggtctacg gcccggcagt taatcagata aattctgaca atattaaaaa ataaatttca
    10321 aataccagaa ggccatttta gttacattta gtaacacata tctgtctgtt taatccacat
    10381 tctgcccccg cagttacgct ttcttatcta ttcttctaat cagcacttca cctgattcat
    10441 ccttaattct aataactggc tattgcgcct tacctcctgt tttgtctttt tttcattacc
    10501 aatgtgtgca tgtgaggaac aatatggcac tgcaaaatga gaaaaatagt cgttatctat
    10561 tacgcgactg gaaaccagaa aatccggcct tctgggaaaa taaaggaaag catattgcac
    10621 gaagaaacct atggatatca gtcagttgtc tacttcttgc cttctgtgtc tggatgctat
    10681 ttagcgcagt tactgttaat ctcaataaaa tcggttttaa tttcactacc gatcaactct
    10741 ttttattaac cgcattaccc tccgtttctg gcgcattatt gcgtgtcccc tactccttta
    10801 tggtgcctat attcggtgga cgccgatgga cggtttttag tactgcaatc ctgattattc
    10861 cttgcgtctg gctcggaatt gccgtgcaga atccgaatac tccttttggg atatttatcg
    10921 ttatcgcttt gctatgcggt tttgcaggtg cgaactttgc ttcgagcatg ggcaatatca
    10981 gtttcttctt tccaaaagcc aaacaaggga gcgctcttgg gattaatggc ggattaggaa
    11041 acttaggtgt aagtgtgatg cagctggttg caccgctggt catttttgta cccgtatttg
    11101 cctttctcgg cgtcaatggc gtaccgcagg ccgacggttc ggtgatgtcg ctggcgaatg
    11161 ccgcatggat ttgggtgcca ttactggcga ttgccacgat cgccgcctgg tcagggatga
    11221 atgatatcgc cagttcacgc gcctcaattg ccgaccagct ccctgtctta caacgcctgc
    11281 atctctggct gctgagcctg ctttatcttg ccaccttcgg ttcgtttatc ggtttttctg
    11341 cgggttttgc catgctggca aaaacccagt tcccggatgt gaatattctg cgcctggcgt
    11401 tctttggccc atttatcggt gccatcgcgc gatcggttgg tggtgctatt tccgataaat
    11461 tcggcggcgt gcgggtgacg ttgatcaact tcatttttat ggcgattttc agcgccctgc
    11521 tgttccttac cttaccgggc acaggctccg gtaatttcat cgcattttac gccgtattta
    11581 tggggctgtt tctgaccgcg ggtctgggaa gtggttctac tttccagatg atcgccgtca
    11641 tctttcgcca gataacaatt tatcgggtga agatgaaagg cggtagtgat gagcaagctc
    11701 aaagagaagc cgtcaccgaa acggcagccg ctctgggctt tatctcagcc attggcgcag
    11761 tgggcggctt ttttattccg caggcgtttg gcatgtcgct caatatgacc ggctctccgg
    11821 tgggcgcgat gaaagtgttt ttaatcttct acatcgtttg tgtgctactg acctggctgg
    11881 tttatggtcg gcggaagttc agccaaaaat aagagatcga tgcctgacac gtcttatcag
    11941 gctacaaccg tgtcgcatcc gacaaggcat cagatcccaa ttcattcctg gagcaggagt
    12001 tatgtcatga gtaaactttt ggatcgcttt cgctacttca aacaaaaggg cgaaaccttt
    12061 gccgatggtc acggacaggt gatgcatagc aaccgcgact gggaggacag ctatcgccag
    12121 cgttggcagt tcgacaaaat cgtgcgttcc acccacggtg ttaactgtac aggctcctgt
    12181 agctggaaaa tctacgttaa aaatggtctg gtgacctggg aaatccaaca gaccgactac
    12241 ccgcgcactc gccctgacct gccaaatcat gaacctcgcg gctgcccgcg tggcgcaagt
    12301 tactcctggt atctctacag cgctaaccgc ctgaaatacc cgctcattcg taaacgactg
    12361 attgaactgt ggcgcgaagc cctcaagcaa cacagcgatc cggtactggc gtgggcatcg
    12421 attatgaacg atccgcaaaa gtgcctgagc tacaaacaag tgcgtggacg cggtggcttt
    12481 atccgctcca actggcagga gctaaatcag ctgattgccg ctgctaacgt ctggaccatc
    12541 aaaacctatg gcccggatcg cgttgccgga ttctcaccga ttccggcgat gtcgatgatt
    12601 tcttacgccg ccggaacacg ctatctgtcg ctgttaggtg gcacctgttt aagcttctac
    12661 gactggtatt gcgacctgcc gcccgcctcg ccgatgacct ggggcgaaca aaccgacgtg
    12721 ccggaatctg ccgactggta taactccagt tacatcatcg cctgggggtc taacgtaccg
    12781 cagacacgta cgccggacgc ccatttcttt accgaagtac gctacaaagg cactaaaacc
    12841 atcgccatta cccctgacta ctctgaagtg gccaaattgt gcgaccagtg gctggcaccg
    12901 aaacaaggca ctgatagtgc cctggcaatg gcaatgggcc atgtcatttt aaaagagttt
    12961 catctcgata atcccagcga ttattttatc aactactgcc gccgctacag cgacatgccg
    13021 atgctggtaa tgctggaacc tcgcgacgat ggcagctacg ttcccgggcg catggtccgc
    13081 gcatctgacc tggtggatgg actgggcgaa agcaataatc cgcagtggaa aaccgtggca
    13141 gttaataccg caggtgaact ggtggtgccg aacggttcga ttggttttcg ctggggagaa
    13201 aaaggcaaat ggaatctgga atccattgcc gccggtacgg aaaccgaatt gtcgttaact
    13261 ctgctcggtc aacatgatgc cgttgcaggc gtggccttcc cctactttgg cggcattgaa
    13321 aatccgcatt ttcgtagcgt aaaacacaat ccggtcctgg tgcgccaatt gcccgttaaa
    13381 aacctgacat tagccgatgg caacacctgt ccggtggtca gcgtttatga tttggtactg
    13441 gcgaattatg gcctcgatcg cgggctggaa gatgaaaaca gcgcgaaaga ttacgctgaa
    13501 atcaaaccgt acaccccagc ctggggtgag caaattaccg gtgtgccgcg ccagtatatt
    13561 gaaaccatcg ctcgtgaatt tgccgatact gcccataaaa cgcatgggcg ctcgatgatt
    13621 atcctcggcg caggtgttaa ccactggtac cacatggaca tgaattaccg tgggatgatc
    13681 aacatgctga tcttctgcgg ttgtgtcggg caaagcggtg gcggctgggc acactatgtc
    13741 ggtcaggaaa aactgcgccc gcaaaccggc tggttgccac tggcctttgc gctcgactgg
    13801 aaccgaccac cgcgccagat gaacagcacc tcgtttttct acaatcattc cagtcaatgg
    13861 cgctatgaaa aagtctctgc tcaggagtta ctttcaccgc tggccgatgc cagtaagtac
    13921 agcggtcatc tgattgattt caacgttcgc gccgaacgta tgggctggct accttctgcg
    13981 ccgcagttgg ggcgtaatcc gctcgggatt aaagctgaag ccgacaaggc cggattatct
    14041 cccacagaat tcaccgccca ggcgctgaaa tcgggcgatt tacgtatggc ctgcgaacaa
    14101 ccagatagcg gcagcaatca tccgcgtaat ttgtttgtct ggcgttctaa cctgcttggc
    14161 tcctccggca aaggtcacga gtatatgcag aagtatctgc tgggtacgga aagtggcatt
    14221 cagggggaag aacttggacc gacggaggga atccaaccgg aagaagttga gtggcaaact
    14281 gcggcgattg aaggcaagct cgacctgctg gtgacgctcg acttccgcat gtccagtacc
    14341 tgtctgttct ccgatatcgt tctgcccacc gccacctggt acgaaaaaga cgatatgaat
    14401 acctcggata tgcatccatt tattcatccg ctttctgcgg cggtcgatcc ggcctgggaa
    14461 tcacgcagcg actgggaaat ctacaaaggt atcgctaaag ccttttcgca agtgtgcgtg
    14521 gggcatctcg gcaaagaaac cgacgtggta ttacaaccac tgctgcatga ctccccggca
    14581 gagctttcac agccgtgtga agtgctcgac tggcgcaaag gtgaatgcga tctgattcca
    14641 ggcaaaaccg cgccgaatat tgtggcggtg gagcgcgact accctgctac ctatgaacgc
    14701 tttacctcac tcgggccatt gatggacaaa cttggcaacg gcggtaaagg gatttcgtgg
    14761 aatacccagg atgaaatcga tttcctcggt aaactcaatt acaccaagcg tgatggcccg
    14821 gcacaggggc gtccgctgat tgataccgcg attgacgcat cagaagtgat tctggcgctg
    14881 gcaccggaaa ccaacggcca tgttgcagtc aaagcgtggc aagcgctggg cgagatcacc
    14941 ggacgcgaac atacccatct ggcgctgcac aaagaggacg agaagattcg ctttcgcgat
    15001 attcaggcgc agccgcgtaa aattatctcc agccccacat ggtctggtct ggaaagcgat
    15061 cacgtttcct ataacgcggg atacaccaac gttcatgagc taattccgtg gcgcacactg
    15121 tcgggacgcc agcaactcta tcaggatcat ccgtggatgc gtgcttttgg tgaaagtctg
    15181 gtggcttatc gcccgcctat cgacacccgt agcgtcagtg aaatgcgcca gataccgcca
    15241 aacggcttcc cggaaaaagc ccttaacttc ctgacgccgc accagaaatg gggcattcac
    15301 tcaacctaca gtgaaaacct gctaatgctg acgctctctc gcggtggacc gattgtctgg
    15361 atcagcgaaa ccgatgcccg agaactgacc attgtcgata acgactgggt ggaagttttt
    15421 aacgccaacg gcgcgctgac tgcccgcgcg gtggtcagcc aacgtgtacc gccgggcatg
    15481 accatgatgt atcacgccca ggaacgcatt atgaatattc ctagttcgga agtaactggc
    15541 atgcgcggcg gcattcataa ctcggttacc cgcgtttgcc cgaaaccaac gcatatgatt
    15601 ggcggttacg cgcagctggc ctggggcttt aactactacg gcaccgtcgg ctcgaaccgc
    15661 gacgaattca ttatgatccg caaaatgaag aacgttaact ggctggatga tgaaggtcgc
    15721 gatcaggttc aggaggcgaa aaaatgaaaa tacgttcgca agtcggcatg gtgcttaacc
    15781 tcgataaatg tatcggctgc catacctgtt cggtgacctg taaaaacgtc tggaccggac
    15841 gcgaaggcat ggagtacgca tggtttaaca acgtcgaaac caaaccgggc attggttatc
    15901 cgaaaaactg ggaagatcag gatgagtggc aaggtggctg ggtgcgtgat gtgaatggca
    15961 agatacgccc gcgtctgggc agcaagatgg gcgtaataac caaaattttc gccaacccgg
    16021 tagtgccgca gattgacgat tactacgaac ctttcacctt cgactacgaa catttgcata
    16081 gcgcgccgga aggcaaacat attcccaccg cccgtccgcg ttcactgatt gacggcaaac
    16141 ggatggacaa agtgatctgg ggcccaaact gggaagaact gctgggcggc gaatttgaaa
    16201 aacgagcccg cgaccgcaac ttcgaggcca tgcaaaagga gatgtacggg cagtttgaaa
    16261 acaccttcat gatgtacctg ccgcgcctgt gcgaacactg cctcaatccc agctgcgtgg
    16321 cgacctgccc cagcggcgct atctacaaac gcgaagaaga cggcattgtg ctgatcgatc
    16381 aggataaatg ccgtggctgg cgtttgtgca ttagcggttg cccatacaaa aaaatctact
    16441 tcaactggaa aagcggtaag tcagaaaaat gtattttctg ttatccgcga attgagtccg
    16501 gtcaaccgac cgtgtgctcc gaaacctgcg tgggtcgcat tcgttatctt ggcgtgctgc
    16561 tctacgacgc cgaccgcatc gaagaagcag cgagtaccga acgagaggtc gatctctatg
    16621 aacgccagtg cgaagtgttc ctcgatccgc acgatccctc ggtgatcgaa gaagccctga
    16681 aacaaggtat tccacaaaac gtgattgacg ctgcccagcg ttcgcctgtc tacaaaatgg
    16741 cgatggactg gaaactggcg ctaccgctgc accctgaata tcgcaccctg ccgatggtct
    16801 ggtacgttcc tccgctgtca ccgattcagt cctacgctga tgcgggaggt ttgccgaaaa
    16861 gcgaaggcgt gctgcccgcc atcgaaagcc tgcgtattcc ggtgcaatat ctcgccaata
    16921 tgttgagtgc cggtgatacc ggtccggtac tgcgggcgct gaaacggatg atggcgatgc
    16981 gccactatat gcgttcacaa accgtggaag gcgttactga tactcgtgcc atcgacgaag
    17041 taggtctgag cgtcgcccag gtcgaagaga tgtatcgtta cctcgccatt gccaactatg
    17101 aagatcgttt cgtcatcccg acaagccatc gggaaatggc gggcgatgcc ttcgcagaac
    17161 gcaacggctg cggttttacc tttggcgacg gttgccacgg ttcggacagc aaattcaacc
    17221 tgttcaacag tagccgtatc gatgccatca acatcaccga agtgcgcgac aaagcggagg
    17281 gcgaataatg cagatcctca aagtgatcgg cttgttgatg gagtatccgg acgagctgtt
    17341 atgggaatgc aaggaggacg cgctggcgtt gatccgccgc gacgcaccga ttcttacgga
    17401 ttttacccgc aacctgctta acgcgccgct gctggataaa caggccgaat ggtgcgaagt
    17461 gtttgaccgt ggacgtacca catcgctgct gctgttcgaa catgttcatg ccgagtcccg
    17521 cgatcgcggc caggcaatgg tggacctgct ggcggagtat gaaaaggtcg gtctgcaact
    17581 gaattgtagg gaactgcccg attatctacc gctgtatctg gagtatttaa gcgtacttcc
    17641 ggacgatcag gcgaaagaag gattactcaa cgttgcgccg atcctcgccc tgcttggcgg
    17701 acgcttaaaa caacgcgagg caccgtggta cgcgttattt gacgccctgt tgcaactggc
    17761 aggaagccct ctgtcaagtg acagtgtcac aaaacagatt cgcagtgaag agcgtgacga
    17821 cacccgccag gcgctggatg cggtgtggga agaagaacag gtgaagttta ttgaagataa
    17881 cgccacggcg tgtgacagct cgccgcttaa tcaatatcag cgacgcttta gccaggatgt
    17941 cgcgccgcag tatgtcgaca tcagtgcggg aggtgggaaa tgattcagta tctgaacgtc
    18001 tttttttacg atatctaccc gtacttgtgt ggcacggttt ttatccttgg aagctggctg
    18061 cgctacgatt acgggcagta tacctggcgt gccgcctcca gccagatgct ggataaacgc
    18121 gggatggtgc tatggtcgaa cttgttccat atcggcattc tggggatctt cttcggtcat
    18181 ctgttcggca tgttaacgcc gcactggatg tacgcgtggt ttctgccggt ggcggtaaaa
    18241 cagcagatgg cgatgatcct cggcggaata tgcggggtat tgacgctgat tggcggtgcg
    18301 gggctactgg tgcgtcgttt aacaaatccg cgtatcaggg tgacgtcttc tggtgcggat
    18361 atcctgatcc tcgccatttt gttaattcag tgcattcttg ggttaaccac cattcctttt
    18421 tcagcacaac atccggacgg cagtgaaatg atgaagttgg tgggctgggc gcaaagcatc
    18481 gtcactttcc agggcggtgc atctacgcat cttgatggtg tcgcctttat attccgcgtt
    18541 catctggtgc ttggaatgac catcttcctg ctcttcccgt tcacccgact ggtgcatgtc
    18601 tggagcgcgc cgtttgagta ctttactcgt cggtatcaga ttgtgcgttc gcggcgttaa
    18661 ttctcacgcg ggccggatag cccttcgctc tccggccact tgcaggctat gatagttctc
    18721 cagccacagg agaacattaa tgaaaccgca ggtttaccac gtcgatgctt ttacctctca
    18781 accgtttcgc ggcaattctg ccggtgttgt tttccccgcc gataatctca gtgaagcgca
    18841 aatgcagctt atcgcccgcg agttaggtca ttcggaaact gcttttctgc tgcacagcga
    18901 cgacagcgat gtgcgcatcc gctactttac gccaacggtt gaagttccga tttgtggtca
    18961 cgcgacagta gctgcgcact atgtacgtgc aaaggtgtta ggtttaggaa attgcacggt
    19021 ctggcaaaca tcgctggcag gaaaacatcg tgtgactata gaaaagcaaa atgatgatta
    19081 tcgtatttcg ctggaacaag gtactccagg ctttgagcca ccgctggaag gtgaaacacg
    19141 tgcagcgatt atcaacgcgc ttcatctcac tgaggacgat attctgccag gcttgccaat
    19201 tcaggtggca acaacaggac actcaaaagt gatgattcca ctgaagccgg aagtggatat
    19261 cgacgccctt tcgcctgacc ttgctgcgct gaccgcaatc agcaagcaga ttggctgcaa
    19321 tggtttcttc ccgttccaga tccgcccagg caaaaacgaa accgatggtc gcatgttctc
    19381 gcctgcgatt ggtattgtgg aagatccggt gactggcaat gccaatggcc cgatgggtgc
    19441 atggttagta catcacaacg tattgcccca cgatggcaac gtgttgcgtg ttaaaggcca
    19501 tcaggggcgc gcattagggc gtgacggcgt gattgaagtg acggtgacaa ttcgtgataa
    19561 ccaaccggag aaagtcacca tttctggcgc ggcggtgatt ttgtttcatg ccgaatgggc
    19621 aattgaactc tgaaaccgtt gcctgatgcg cttcgcttat caggtctaca accgggctaa
    19681 tgtaggccgg ataaggcgtt
//
LOCUS       DADSYM010000089        19647 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2415
            SAMN08039579-rid11723173.denovo.089, whole genome shotgun sequence.
ACCESSION   DADSYM010000089 DADSYM010000000
VERSION     DADSYM010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039579
            Sequence Read Archive: SRR6362533
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 19647)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19647)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980162.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 03:39:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,484
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 4,347
            CDSs (with protein)               :: 4,347
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 43 of 85
            Pseudo Genes (incomplete)         :: 37 of 85
            Pseudo Genes (internal stop)      :: 19 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19647
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039579-rid11723173.denovo.089"
                     /strain="LGE2415"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8302 N 95.9628 W"
                     /collection_date="2015-08-24"
     gene            complement(379..2043)
                     /gene="asnB"
                     /locus_tag="J1Y83_002739"
     CDS             complement(379..2043)
                     /gene="asnB"
                     /locus_tag="J1Y83_002739"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001724523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase B"
                     /protein_id="HBA7630874.1"
                     /translation="MCSIFGVFDIKTDAVELRKKALELSRLMRHRGPDWSGIYASDNA
                     ILAHERLSIVDVNAGAQPLYNQQKTHVLAVNGEIYNHQALRAEYGDRYQFQTGSDCEV
                     ILALYQEKGPEFLDDLQGMFAFALYDSEKDAYLIGRDHLGIIPLYMGYDEHGQLYVAS
                     EMKALVPVCRTIKEFPAGSYLWSQDGEIRSYYHRDWFDYDAVKDNVTDKNELRQALED
                     SVKSHLMSDVPYGVLLSGGLDSSIISAITKKYAARRVEDQERSEAWWPQLHSFAVGLP
                     GSPDLKAAQEVANHLGTVHHEIHFTVQEGLDAIRDVIYHIETYDVTTIRASTPMYLMS
                     RKIKAMGIKMVLSGEGSDEVFGGYLYFHKAPNAKELHEETVRKLLALHMYDCARANKA
                     MSAWGVEARVPFLDKKFLDVAMRINPQDKMCGNGKMEKHILRECFEAYLPASVAWRQK
                     EQFSDGVGYSWIDTLKEVAAQQVSDQQLETARFRFPFNTPTSKEAYLYREIFEELFPL
                     PSAAECVPGGPSVACSSAKAIEWDEAFKKMDDPSGRAVGVHQSAYK"
     gene            complement(2298..3050)
                     /gene="nagD"
                     /locus_tag="J1Y83_002740"
     CDS             complement(2298..3050)
                     /gene="nagD"
                     /locus_tag="J1Y83_002740"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide monophosphatase NagD"
                     /protein_id="HBA7630875.1"
                     /translation="MTIKNVICDIDGVLMHDNVAVPGAAEFLHGIMDKGLPLVLLTNY
                     PSQTGQDLANRFATAGVDVPDSVFYTSAMATADFLRRQEGKKAYVVGEGALIHELYKA
                     GFTITDVNPDFVIVGETRSYNWDMMHKAAYFVANGARFIATNPDTHGRGFYPACGALC
                     AGIEKISGRKPFYVGKPSPWIIRAALNKMQAHSEETVIVGDNLRTDILAGFQAGLETI
                     LVLSGVSSLDDIDSMPFRPSWIYPSVAEIDVI"
     gene            complement(3098..4318)
                     /gene="nagC"
                     /locus_tag="J1Y83_002741"
                     /gene_synonym="nagR"
     CDS             complement(3098..4318)
                     /gene="nagC"
                     /locus_tag="J1Y83_002741"
                     /gene_synonym="nagR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NagC"
                     /protein_id="HBA7630876.1"
                     /translation="MTPGGQAQIGNVDLVKQLNSAAVYRLIDQYGPISRIQIAEQSQL
                     APASVTKITRQLIERGLIKEVDQQASTGGRRAISIVTETRNFHAIGVRLGRHDATITL
                     FDLSSKVLAEEHYPLPERTQQTLEHALLNAIAQFIDSYQRKLRELIAISVILPGLVDP
                     DSGKIHYMPHIQVENWGLVEALEERFKVTCFVGHDIRSLALAEHYFGASQDCEDSILV
                     RVHRGTGAGIISNGRIFIGRNGNVGEIGHIQVEPLGERCHCGNFGCLETIAANAAIEQ
                     RVLNLLKQGYQSRVPLDDCTIKTICKAANKGDSLASEVIEYVGRHLGKTIAIAINLFN
                     PQKIVIAGEITEADKVLLPAIESCINTQALKAFRTNLPVVRSELDHRSAIGAFALVKR
                     AMLNGILLQHLLEN"
     gene            complement(4327..5475)
                     /gene="nagA"
                     /locus_tag="J1Y83_002742"
     CDS             complement(4327..5475)
                     /gene="nagA"
                     /locus_tag="J1Y83_002742"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HBA7630877.1"
                     /translation="MYALTQGRIFTGHEFLDDHAVVIADGLIKSVCPVAELPPEIEQR
                     SLNGAILSPGFIDVQLNGCGGVQFNDTAEAVSVETLEIMQKANEKSGCTNYLPTLITT
                     SDELMKQGVRVMREYLAKHPNQALGLHLEGPWLNLVKKGTHNPNFVRKPDAALVDFLC
                     ENADVITKVTLAPEMVPAEVISKLANAGIVVSAGHSNATLKEAKAGFRAGITFATHLY
                     NAMPYITGREPGLAGAILDEADIYCGIIADGLHVDYANIRNAKRLKGDKLCLVTDATA
                     PAGANIEQFIFAGKTIYYRNGLCVDENGTLSGSSLTMIEGVRNLVEHCGIALDEVLRM
                     ATLYPARAIGVEKRLGTLAAGKVANLTAFTPDFKITRTIVNGNEVVTQ"
     gene            complement(5535..6335)
                     /gene="nagB"
                     /locus_tag="J1Y83_002743"
     CDS             complement(5535..6335)
                     /gene="nagB"
                     /locus_tag="J1Y83_002743"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008805656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HBA7630878.1"
                     /translation="MRLIPLTTAEQVGKWAARHIVNRINAFKPTADRPFVLGLPTGGT
                     PMTTYKALVEMHKAGQVSFKHVVTFNMDEYVGLPKEHPESYYSFMHRNFFDHVDIPAE
                     NINLLNGNAPDIDAECRQYEEKIRSYGKIHLFMGGVGNDGHIAFNEPASSLASRTRIK
                     TLTHDTRVANSRFFDNDVNQVPKYALTVGVGTLLDAEEVMILVLGSQKALALQAAVEG
                     CVNHMWTISCLQLHPKAIMVCDEPSTMELKVKTLRYFNELEAENIKGL"
     gene            6668..8614
                     /gene="nagE"
                     /locus_tag="J1Y83_002744"
     CDS             6668..8614
                     /gene="nagE"
                     /locus_tag="J1Y83_002744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetyl glucosamine transporter subunit
                     IIABC"
                     /protein_id="HBA7630879.1"
                     /translation="MNILGFFQRLGRALQLPIAVLPVAALLLRFGQPDLLNVAFIAQA
                     GGAIFDNLALIFAIGVASSWSKDSAGAAALAGAVGYFVLTKAMVTINPEINMGVLAGI
                     ITGLVGGAAYNRWSDIKLPDFLSFFGGKRFVPIATGFFCLVLAAIFGYVWPPVQHAIH
                     AGGEWIVSAGALGSGIFGFINRLLIPTGLHQVLNTIAWFQIGEFTNAAGTVFHGDINR
                     FYAGDGTAGMFMSGFFPIMMFGLPGAALAMYFAAPKERRPMVGGMLLSVAVTAFLTGV
                     TEPLEFLFMFLAPLLYLLHALLTGISLFVATLLGIHAGFSFSAGAIDYALMYNLPAAS
                     QNVWMLLVMGVVFFAIYFVVFSLVIRMFNLKTPGREDKEDEIVTEEANSNTEEGLNQL
                     ATNYIAAVGGTDNLKAIDACITRLRLTVVDSARVNDAMCKRLGASGVVKLNKQTIQVI
                     VGAKAESIGDAMKKVVARGPVAAASSEATPATAAPVAKPQAVPNAVSIAELVSPITGD
                     VVALDQVPDEAFASKAVGDGVAVKPTDKIVVSPAAGTIVKIFNTNHAFCLETEKGAEI
                     VVHMGIDTVALEGKGFKRLVEEGAQVSAGQPILEMDLDYLNANARSMISPVVCSNIDD
                     FSGLIIKAQGHVVAGQTPLYEIKK"
     gene            complement(8703..9983)
                     /locus_tag="J1Y83_002745"
     CDS             complement(8703..9983)
                     /locus_tag="J1Y83_002745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000177666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane transport protein"
                     /protein_id="HBA7630880.1"
                     /translation="MTMEMILALGILVLMIVLIMSDKMPFGAPPLLACLLLVVSGLST
                     VQQAFAGFVNPSVVMIAGFMVVMAALQKTRLISNVKSAMISLVNKGSYRSYGLLLVIV
                     MLGASLAGTGATGYYVLILSLVSTIPYSKKLPTSKLMMPLGFATNHPLLPINLALLFG
                     VTATVLETAGFHQEISMGRFALVNLIMSAAFLAWSLIAYRFLPDHPIADASEDALAAR
                     EETFNALPAWKEYCTIAAFAVSVIGMMLMNILGNIAFVIPGLAGAFVLMIDVLDFKEV
                     RDHMGAPVILMMAGVIGVADALAGTGFTAMVGDAVAGVLGSGVSPFVFIVAFALLTST
                     CATFTGSNMGSVYIFAPIAIAACTSLGLNPTAAAIAVVISGWNGGYMPIDGMPAMILG
                     MGKYKLPEFWVFSVPMYLIRILALCAGAVFIFPM"
     gene            complement(9997..10467)
                     /locus_tag="J1Y83_002746"
     CDS             complement(9997..10467)
                     /locus_tag="J1Y83_002746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015964430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HBA7630881.1"
                     /translation="MIIGNLNHLSLAGLPAWVRNILLRPECSLSALSTREDGRWQPEG
                     CRWFCTLGTSDTQPAELRHTEYHHLWADIQVVITGCEGINAGTRPIARENDEERKPDL
                     FIAPSPENSVAITLHAGDFAVFMPEEPHQALCAIGTATSIRKAVFKVPRDMLEA"
     gene            complement(10464..11633)
                     /locus_tag="J1Y83_002747"
     CDS             complement(10464..11633)
                     /locus_tag="J1Y83_002747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000109428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HBA7630882.1"
                     /translation="MSVALIRDGVIRPSENDSHVLTAMLPSSCPQNHAANILPLPDGA
                     LMCVWFAGTQEGIADISVWGSRLPAGGMQWSDAVKLSHDDTRSEQNPVLFLAPDNVLW
                     LLWTAQISGNQDTAIVRYRKSDDLGQTWGEIATLLDKPGTFIRQPITVLDNGNWLLPV
                     FYCRTQPGEKWVGNDDISAVKISADGGHSWRDVEVPQSLGCVHMNITMLHNGTLVALF
                     RSRWADNIYISHSADNGESWSVPQATELPNNNSSIQVTTLASGELALVYNAMSAAGAV
                     ERRASLYDEIDDGDDSRKEPTAVGRSTFWGAPRAPMTVAISADGGKSWPWRRNLDEGD
                     GYCMTNNSLEKLNREFSYPSIKQSPDGTLHIAYTWWRQAIKYVRISPEWVKGQAS"
     gene            complement(11669..12427)
                     /locus_tag="J1Y83_002748"
     CDS             complement(11669..12427)
                     /locus_tag="J1Y83_002748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001524287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HBA7630883.1"
                     /translation="MFLWFMQPDIWLTLITLTFLEIILGVDNIIFLSLVVAKLPLSQQ
                     NMARKLGLSCAMIMRILLLISIAWLSHITQPLFTVLTLEISFRTLILLGGGLFLIYKA
                     IQEIREELSPAQEDEHGSGSRQLSLCGAIVQIMLLDIVFSLDSVITAVGLSQHIFIMI
                     AAVMIAVGVMMFAAKTIGDFVNATPSIKILALTFLLFVGVLLVADSLNIHIAKEYLYF
                     AIFFSLSVETLNIIRERRNKKVSTLIETHESHSH"
     gene            complement(12448..13375)
                     /locus_tag="J1Y83_002749"
                     /pseudo
     CDS             complement(12448..13375)
                     /locus_tag="J1Y83_002749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001126955.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
     gene            complement(13379..14536)
                     /locus_tag="J1Y83_002750"
     CDS             complement(13379..14536)
                     /locus_tag="J1Y83_002750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000234754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HBA7630884.1"
                     /translation="MVIINSTILSGAGAIPSLTSLLPDIRKMLLVTDRNIAQLDGVQQ
                     IRALLEKHCPQVNVIDNVPAEPTHHDVRQLMDVLGDASFDVVVGIGGGSVLDVAKLLS
                     VLCHPQSPGLDALLAGEKPTQRVQSWLIPTTAGTGSEATPNAILAIPEQSTKVGIISQ
                     VLLPDYVALFPELTTSMPAHIAASTGIDALCHLLECFTATVANPVSDNAALTGLSKLF
                     RHIQPAVNDPQDLRAKLEMLWASYYGGVAITHAGTHLVHALSYPLGGKYHLPHGVANA
                     ILLAPCMAFVRPWAVEKFARVWDCIPDAETALSAEEKSHALVTWLQALVNQLKLPNNL
                     AALGVPPEDIASLSEAALNVKRLMNNVPCQLDLQDVQAIYQTLFPQHQFKE"
     gene            14706..15941
                     /locus_tag="J1Y83_002751"
     CDS             14706..15941
                     /locus_tag="J1Y83_002751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016238327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four-carbon acid sugar kinase family protein"
                     /protein_id="HBA7630885.1"
                     /translation="MEKGITKPSILVVADDFTGANDAGVSLAQVGHTVDVAFEMHYRG
                     DASVWVINSDSRAMDPKLAAMKITSLMSHLPLANNPPLVIKKIDSTLRGNIGAEIEAL
                     MKACGITGAVVAPAFPQAGRTTVAGECWVNGVRITETEFASDPKTPVLSARIADIIRL
                     QTAIPCQPVTVSQLSHLSYEQPWISVIDAQTDSDLDRIAAAVMQAKQPLLLVGSAGIC
                     DAVARRSAIMPPPTVLAIIGSMSEIAQRQIATLHSHPRITQIYVDVEHILAGNASDYD
                     ARIVQALQKGDHCIVHTCNDSVARHQIDTLCQRWQMSRAALGEKICRFLGELTRQVLL
                     RTMPDALYLSGGDVAMATASALGATGFRITGKVAQCVPYGHFLGGVWSRSVMTKAGGF
                     GDETTLHQVLNFIEEKCSE"
     gene            15934..16920
                     /locus_tag="J1Y83_002752"
     CDS             15934..16920
                     /locus_tag="J1Y83_002752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001059575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-threonate 4-phosphate dehydrogenase"
                     /protein_id="HBA7630886.1"
                     /translation="MNNIIAVTMGDPAGIGPEIIIKSLSEAGLAGAPVVVVGCVRTLR
                     RIMAMNITPQAELRVIQKVCDAHFAPGVINVMDEPLANPDALTPGVVQAVAGDLAYRC
                     IKRATALALSGEVKAIATAPLNKEALHLGGHNYPGHTELLAHLTASKEYAMVLYTDKL
                     KVIHISTHISLRKFLDTLNGERVKTVIRVANHFLKRVGIERPRIAVAGVNPHAGEHGL
                     FGTEEIEIIAPAIEAMQAEDIDVTGPCPPDTVFMQCHEGLFDMVVAMYHDQGHIPLKL
                     LGFYDGVNITAGLPFIRTSADHGTAFDIAWTGKAKSESMAVSIQLAMQISRE"
     gene            16922..17683
                     /locus_tag="J1Y83_002753"
     CDS             16922..17683
                     /locus_tag="J1Y83_002753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HBA7630887.1"
                     /translation="MKGYNRLEQIMDYLKSHNLVTVDELVAVTNASPATIRRDLIKLD
                     EQGVISRTHGGVTLNRFIPTQPTTHEKMQRSLAEKHAIASAAASMVKAGDSVVLDAGT
                     TMIELARQITHLPLRVITSDLHIALFLAEFKQIEVTIIGGRIDDSSQSCIGEHGRKLL
                     QNTWPDVAFLSCNSWDLEKGITAPTEEKAALKRDLIAHASRKILLADSSKYGSWSLFN
                     IAHLNELTEIITDSQLDGQTRRTLASLSTRLIIAD"
     gene            17903..19567
                     /gene="glnS"
                     /locus_tag="J1Y83_002754"
     CDS             17903..19567
                     /gene="glnS"
                     /locus_tag="J1Y83_002754"
                     /EC_number="6.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase"
                     /protein_id="HBA7630888.1"
                     /translation="MSEAEARPTNFIRQIIDEDLASGKHTTVHTRFPPEPNGYLHIGH
                     AKSICLNFGIAQDYKGQCNLRFDDTNPVKEDIEYVDSIKNDVEWLGFHWSGNVRYSSD
                     YFDQLHAYAIELINKGLAYVDELTPEQIREYRGTLTQPGKNSPYRDRSVEENLALFEK
                     MRTGGFEEGKACLRAKIDMASPFIVMRDPVLYRIKFAEHHQTGNKWCIYPMYDFTHCI
                     SDALEGITHSLCTLEFQDNRRLYDWVLDNITIPVHPRQYEFSRLNLEYTVMSKRKLNL
                     LVTDKHVEGWDDPRMPTISGLRRRGYTAASIREFCKRIGVTKQDNTIEMASLESCIRE
                     DLNENAPRAMAVIDPVKLVIENYQGEGEMVTMPNHPNKPEMGSRQVPFSGEIWIDRAD
                     FREEANKQYKRLVLGKEVRLRNAYVIKAERVEKDAEGNITTIFCTYDADTLSKDPADG
                     RKVKGVIHWVSAAHALPVEIRLYDRLFSVPNPGAADDFLSVINPESLVIKQGFAEPSL
                     KDAVAGKAFQFEREGYFCLDSRHSTAEKPVFNRTVGLRDTWAKVGE"
BASE COUNT         4788 a         5007 c         5129 g         4723 t
ORIGIN      
        1 cttttttttc gcgatacctt atcggcgttg cggggcgcat tatgcgtata gagccttgca
       61 gcgtcaacct ctttttcaag gaaaattgct cgaaagtgac tgtttggtta ggttgcgaac
      121 agcatggcgc tatattcgtc aattattgtt tactttgtgt tttttcccca ccctacagcc
      181 attcttttgt catacaggat gaaattcgga attcaacaat agtggtggga aattaatcta
      241 tgaaatactg gcctacagta atgagttgtc aaacagcgat gtggcaaacc cggaacattt
      301 ccttactgca tatcagaatc aacaagcgcc ccagtaactg aaattgcccc ggatttctcc
      361 ggggcaatat tacacgtctt atttatatgc cgactggtga acgccaaccg cgcgaccaga
      421 cggatcgtcc attttcttga acgcttcatc ccactcaatc gctttagcgg aagaacaagc
      481 gacggaagga ccgcccggca cgcactcagc ggcgctcgga agcgggaaca attcttcaaa
      541 gatctcccgg tacagatacg cttcttttga ggtcggcgtg ttgaacggga agcggaagcg
      601 ggcagtttcc agctgttgat cagaaacctg ctgcgctgcc acttctttca gggtgtcgat
      661 ccaactgtaa ccgacgccat cggagaactg ctctttttgc cgccaggcca cgcttgcagg
      721 cagatacgct tcaaaacatt cacgcaggat gtgtttttcc attttgccgt taccgcacat
      781 tttatcctgt gggttaatgc gcatcgccac atcaaggaat tttttgtcga ggaacggaac
      841 gcgtgcttcc acgccccagg ctgacatcgc tttgttggca cgcgcgcagt catacatatg
      901 cagggccagc agtttacgca ccgtctcttc atgcaactct ttagcgttcg gcgctttgtg
      961 gaagtataga taaccgccaa acacttcgtc agaaccttca ccggacagca ccattttaat
     1021 gcccatcgcc ttgatcttac gcgacattaa atacatcggt gttgaagcgc gaatagtggt
     1081 cacatcataa gtttcgatgt ggtaaatcac gtcgcggatg gcatccagac cttcctgtac
     1141 agtgaagtga atttcgtgat gcaccgtgcc cagatggttt gccacttcct gggctgcttt
     1201 cagatccggt gaacccggca gacctacagc aaaggagtgc aactgcggcc accaggcttc
     1261 agagcgttcc tgatcttcca cgcgacgggc ggcgtatttc ttggtgatag cggaaataat
     1321 tgaggaatcc agaccaccag aaagcagcac accgtaaggc acatcagaca tcagatggct
     1381 tttaacggaa tcttccagtg cctgacgcag ctcgtttttg tcggtcacgt tatctttcac
     1441 cgcatcgtag tcgaaccagt cgcgatgata ataagaacgg atttcgccgt cctgactcca
     1501 caaatagctc cccgccggaa actctttaat cgtgcggcaa actggcacga gggctttcat
     1561 ttctgaggcc acatacagct gaccgtgttc gtcatagccc atatacagtg ggatgatccc
     1621 cagatggtcg cgaccaatca gatatgcatc tttttcgctg tcgtacaggg caaaggcaaa
     1681 catgccctgc aagtcgtcga gaaattccgg ccctttttcc tgatacagcg cgaggatcac
     1741 ttcacagtca gacccggtct ggaactggta acgatcgcca tattcagcgc gcaatgcctg
     1801 gtggttgtag atttcaccgt ttactgccag cacatgagtt ttttgttggt tgtagagagg
     1861 ttgcgccccc gcgttaacgt caacaattga cagacgttcg tgggcgagga tggcgttatc
     1921 gctggcataa ataccggacc agtccgggcc acgatgacgc atcaggcgtg acagctcgag
     1981 tgctttctta cgcagctcaa ctgcgtctgt tttgatatcg aatacgccaa aaatagaaca
     2041 cataaccttc tccgttaacc tggtgtttac tgcgtgttgt gtttgcttgt taaaaaaatg
     2101 ccgcaaagca gcacggtgcg caagcgattt ggcagtggaa aaataaaaaa cgtaatggcg
     2161 attgctgatt gatgaaaaag gggagggctt ttgggtatat ttattgatgg atcgataatt
     2221 tttgacgggt tttattgaat ggggtgattt taatgatggg taaaaataag aaaccacgcc
     2281 ctgaggcgtg gttgctttca gataacgtcg atttcagcga ccgatgggta aatccaactt
     2341 gggcggaacg gcatactgtc gatatcgtcg agtgacgaaa caccagaaag caccagaatc
     2401 gtctccagac cggcctggaa gcccgccaga atatcggtac gcaagttatc gccgacaatc
     2461 accgtttctt ccgaatgcgc ctgcattttg ttcaatgccg cgcggatgat ccacggactc
     2521 ggctttccaa catagaacgg tttgcgcccg gagattttct cgatccctgc acacaacgcg
     2581 ccacaggcgg gataaaaacc gcgcccgtgg gtgtccgggt tggtggcgat aaagcgtgca
     2641 ccgttagcga cgaaataggc tgctttatgc atcatgtccc agttgtagga acgcgtttcg
     2701 ccaacaatca caaaatcagg gttcacatcg gtaatagtga aaccggcttt gtacagttca
     2761 tgaatcagtg cgccttcgcc caccacatac gctttcttgc cttcctggcg acgcaggaaa
     2821 tcggcggtcg ccatcgcgga ggtataaaac acgctatccg gtacatcgac accggcagtg
     2881 gcaaagcgat tcgccagatc ttgcccggtc tgcgaaggat agttggtcag caacaccagc
     2941 ggcaggcctt tatccataat cccgtgcaaa aattccgctg caccgggtac ggcgacgtta
     3001 tcgtgcatca gcacgccgtc gatatcgcaa attacatttt taatggtcat ggactaccca
     3061 gaatattgac aacaataagc gccactataa aagcacatta attttccagc aaatgctgga
     3121 gcaaaatacc gttgagcatg gcgcgtttta ccagcgcaaa agcgccgatt gccgagcggt
     3181 gatccaactc agaacgtacc accggtagat tagtacgaaa cgccttcaac gcctgggtat
     3241 taatgcagct ttcaatagca gggagcagca ctttatcggc ttcggtgatt tctccagcaa
     3301 taacaatttt ttgcggatta aataagttga tagcaatggc gatggtttta cccagatgac
     3361 gaccgacata ttcaatcact tccgaggcca ggctatcgcc tttgttcgcg gctttgcaga
     3421 tagttttgat ggtgcagtcg tccagtggca cacggctctg gtagccctgc tttaacagat
     3481 tcaacacccg ttgttcaatg gcagcattgg cggcgatagt ttccaggcag ccaaagttgc
     3541 cgcagtggca gcgttcaccc agcggttcga cctgaatatg gccaatttca ccgacgttgc
     3601 cgttgcgccc gataaaaatt cgcccgttag agataatccc ggccccggtt ccgcgatgga
     3661 cacgcaccag aatggagtct tcgcaatcct gacttgcacc gaagtagtgc tctgccagcg
     3721 ccagactgcg gatatcgtgg ccgacaaaac aggtcacttt aaaacgctct tccagtgctt
     3781 caaccagccc ccagttttct acctgaatat gcggcatgta atgaattttt ccgctgtccg
     3841 ggtctacaag ccctggcagg atcaccgaaa tcgcgatcag ctcgcgtagt ttgcgctggt
     3901 agctatcaat aaactgagca atggcattca gcagggcatg ttccagcgtt tgctgagtgc
     3961 gttcaggcaa cgggtaatgt tcttctgcca acactttgct gctgagatca aacaaggtga
     4021 tggtggcgtc gtgacgacca agccgtacgc cgattgcgtg gaaattgcgg gtttcggtga
     4081 cgatggagat agcgcggcgg cccccggtgg aggcctgctg atcaacttct ttgatcagcc
     4141 cgcgttcgat aagctgacgc gtaattttgg ttacgctggc gggggcaagc tgactttgct
     4201 cggcaatctg aatccgcgag attggcccgt actggtcaat caggcgataa actgccgcgc
     4261 tgttaagctg ttttacgaga tcaacattac ctatctgagc ttgtccgcct ggtgtcatac
     4321 tttctcttat tgagttacga cctcgttacc gttaacgatg gtcctggtga ttttaaaatc
     4381 aggtgtgaat gcagtcaggt tggctacttt acctgcggcg agtgtaccga gacgtttctc
     4441 aacgccaatc gcacgcgccg gatagagcgt cgccatacgc agcacttcat ccagtgcgat
     4501 accgcaatgt tcgaccagat tacgcacgcc ttcaatcatg gttaaggatg aaccgcttaa
     4561 cgtaccgttc tcatccacac aaagtccatt acggtagtat attgttttac ccgcaaaaat
     4621 gaactgttca atgttggcac ctgctggcgc ggtggcgtca gtaaccagac acagtttgtc
     4681 gcctttcaga cgtttagcgt tgcgaatgtt ggcgtaatca acatgcaggc catcagcgat
     4741 aataccgcaa taaatgtcag cttcgtcgag gatcgcgccc gccaggcccg gttcacgacc
     4801 ggtaatatac ggcatcgcgt tgtacagatg ggtggcaaag gtaatccccg cgcggaaacc
     4861 ggcttttgct tctttcaacg tcgcgttgga gtgaccagca gaaaccacaa tcccggcatt
     4921 tgccagtttg ctgatgactt ccgctggaac catttccggt gccagggtca ctttggtaat
     4981 gacatcggca ttttcgcaca ggaaatcgac cagcgcggca tcaggcttac gcacaaaatt
     5041 cggattatgg gtgccttttt ttaccagatt cagccacgga ccttccagat gcagacctaa
     5101 cgcctgattc ggatgttttg ccaggtactc gcgcataacg cgcacgccct gtttcatcag
     5161 ctcgtcgctg gtggtgataa gcgtcggcaa atagttagta cagcctgatt tctcattggc
     5221 tttctgcatg atttccagcg tttctacgct gactgcttca gcagtatcgt taaactgcac
     5281 gccgccgcag ccgtttaact gcacatcgat aaaaccgggg gagagaatgg ccccgttcag
     5341 tgaacgttgt tcgatctctg gcggcagttc cgctaccgga cagacgcttt taatcaggcc
     5401 atcagcgata acaaccgcgt ggtcatcaag aaattcgtgg ccggtaaaga tccggccctg
     5461 ggttaatgca tacattctga cccccgattt taaaaaatat tgccctgagc aaggagccag
     5521 ggcagggata acaattacag acctttgata ttttctgctt ctaattcatt gaaatatctt
     5581 aaagtcttaa ctttcagctc catggtggaa ggttcatcgc acaccatgat cgcttttgga
     5641 tgcagttgca gacagctgat ggtccacatg tggttcacgc aaccttcaac ggcggcctgc
     5701 agtgccagtg ctttctggct acccagcacc agaatcatca cttcttcggc atccagcaat
     5761 gtaccaacac cgacagtcag ggcatatttt ggcacctgat taacatcgtt atcaaagaaa
     5821 cgagagttcg cgacgcgagt gtcatgagtc agggttttga tacgagtacg agaagccaga
     5881 gaagacgccg gttcgttaaa tgcaatatga ccgtcgttac ctacaccgcc cataaacaga
     5941 tgaatttttc cgtaagaacg gattttttct tcatactggc ggcattcggc gtcgatatcc
     6001 ggggcgttgc cgttgagaag gttgatattt tctgctggaa tatcaacgtg atcgaagaaa
     6061 ttacggtgca taaagctgta gtagctttcc ggatgctctt tcggcagacc gacatattcg
     6121 tccatgttga aggtgacaac gtgcttaaag ctgacctggc ctgctttatg catttcgact
     6181 aacgctttat aggtggtcat cggcgtgccg ccagtcggca ggcccagtac aaacggacga
     6241 tcggcagtcg gtttgaacgc attgatacga ttgacgatat ggcgagcagc ccatttgccg
     6301 acctgttcag cggtagtcag ggggatcagt ctcattattc acctcaataa gtaaaatgta
     6361 agccgttggc ggattaggca tctttaagcg taacctggat ttgcgcagac aggcgtcaat
     6421 ccgacctgat tttttgaatg ataaaataag ttttctggtt tagccagtaa aagggagtga
     6481 tgataacgat atttggtgac aaaactcaca aaagacacgc gtttaatttg cgatacgaat
     6541 taaattttca cacactctgt agcagatgat ctaacaatct gattacagaa catcggcagt
     6601 acaatttgca gcaaaataaa aatacggctt taaacgagcc aaatagggtt ctcgtagggg
     6661 gaataagatg aatattttag gttttttcca gcgactcggt agggcgttac agctccctat
     6721 cgcggtgctg ccggtggcgg cgctgttgct gcgattcggt cagccagatt tacttaacgt
     6781 tgcgtttatt gcccaggcgg gcggtgcgat ttttgataac ctcgcattaa tcttcgccat
     6841 cggcgtggca tccagctggt cgaaagacag cgcaggtgcg gcggcactgg cgggtgcggt
     6901 aggttacttt gtgttaacca aagcgatggt gaccatcaac ccagaaatta acatgggtgt
     6961 actggcgggt atcattaccg gtctggttgg tggcgcagcc tataaccgtt ggtccgatat
     7021 taaactgccg gacttcctga gcttcttcgg cggcaaacgc tttgtgccga tcgccaccgg
     7081 cttcttctgt ctggtgctgg cggccatttt tggttacgtc tggccaccgg tacagcacgc
     7141 tatccatgca ggcggcgagt ggatcgtttc tgcgggcgcg ctgggttccg gtatctttgg
     7201 tttcatcaac cgtctgctga tcccaaccgg tctgcatcag gtgctgaaca ccatcgcctg
     7261 gttccagatt ggtgaattca ccaacgcggc gggaacggtt ttccacggtg atatcaaccg
     7321 cttctatgcc ggtgacggca ccgcggggat gttcatgtcc ggcttcttcc cgattatgat
     7381 gttcggtctg ccgggtgcgg cgctggcgat gtacttcgca gcaccgaaag agcgtcgtcc
     7441 gatggttggc gggatgctgc tttctgttgc tgttactgcg ttcctgaccg gtgtgactga
     7501 gccgctggaa ttcctgttca tgttccttgc accgctgctg tacctcctgc acgcactgct
     7561 gaccggtatc agcctgtttg tggcaacgct gctgggtatc catgccggct tctctttctc
     7621 tgcgggggct atcgactacg cgttgatgta taacctgccg gccgccagcc agaacgtctg
     7681 gatgctgctg gtgatggggg ttgtcttctt cgctatctac ttcgtggtgt tcagtttggt
     7741 tatccgcatg ttcaacctga aaacgccggg tcgtgaagat aaagaagacg agatcgttac
     7801 tgaagaggct aacagcaata ctgaagaagg tctcaatcaa ctggcaacca actatattgc
     7861 tgcggttggc ggtactgaca acctgaaagc cattgatgcc tgtattactc gtctgcgcct
     7921 taccgtggtt gactcagctc gcgtcaacga tgcgatgtgt aaacgtctgg gtgcttctgg
     7981 ggtagtgaaa ctgaacaaac agactattca ggtgattgtt ggcgcgaaag cagaatctat
     8041 cggcgatgcg atgaagaaag tcgttgcccg tggtccggta gccgctgcgt catctgaagc
     8101 aactccggca actgccgctc ctgtagcaaa accgcaggct gtaccaaacg cggtatctat
     8161 cgcggagctg gtatcgccga ttaccgggga tgttgtggca ctggatcagg ttcctgacga
     8221 agcattcgcc agcaaagcgg tgggtgacgg tgtggcggtg aaaccgacag ataaaatcgt
     8281 cgtatcacca gccgcaggaa caatcgtgaa aatcttcaac accaaccacg cattctgtct
     8341 ggaaaccgaa aaaggcgcgg agatcgtcgt ccatatgggt atcgacaccg tagcgctgga
     8401 aggtaaaggc tttaaacgtc tggtggaaga gggcgcgcag gtaagcgcag ggcaaccgat
     8461 tctggaaatg gatctggatt acctgaacgc taacgctcgc tcgatgatta gcccggtggt
     8521 ttgcagcaat atcgacgatt tcagcggcct tatcattaaa gctcagggcc atgttgtggc
     8581 gggtcaaaca ccgctgtatg aaatcaaaaa gtaatctgct ttatgtctga tgcgacgcgg
     8641 cagcgtcgca tccaacaata aaaagcggca gggatcttct ctgccgcttt ttttgtctga
     8701 gattacattg ggaagataaa aacagcgcct gcacaaagcg ccagaatgcg gatgagatac
     8761 attggtacag agaacaccca gaattcggga agtttgtatt tccccattcc gaggatcatg
     8821 gctggcattc cgtcaatagg catatagccg ccattccagc cagaaatcac caccgcaatc
     8881 gcggcagcgg tcgggttaag cccaaggcta gtacaggcgg caatggcgat gggggcaaaa
     8941 atatatactg accccatatt cgaacccgtg aaggtggcgc aggtactggt aagcaaagca
     9001 aaggcgacaa taaatacaaa cggtgagacg ccagaaccga gcacacctgc aacggcatcg
     9061 ccaaccatcg ccgtaaagcc ggtgcccgcc agtgcatcag ctacaccgat cacccccgcc
     9121 atcattaaaa tgaccggagc ccccatatga tcgcgaactt ctttgaagtc cagtacatcg
     9181 atcatcagaa caaacgcacc cgcaagaccc ggaataacga aggcgatatt tcccagaatg
     9241 ttcattagca tcatgccgat aacgctgaca gcgaatgcgg caatagtgca atattctttc
     9301 caggcaggaa gggcgttaaa cgtttcctcg cgtgccgcca gcgcatcctc ggaagcatcc
     9361 gcaatgggat gatccggaag aaaacggtag gcaataagac tccatgcgag gaaagccgct
     9421 gacatgatga gattcaccag cgcaaagcgc cccatcgaaa tctcctgatg aaaccccgcg
     9481 gtttcgagta cggtcgccgt aacgccaaag agaagcgcga gattgatagg aagaagcggg
     9541 tggttggtgg caaatcccag gggcatcatt aatttcgatg ttggtagctt tttactgtac
     9601 ggaatggtgg ataccagcga cagaattagc acgtaataac cggtcgctcc ggtgccggca
     9661 agactggcac ccagcataac aatgactaac aacagcccat agctgcggta actgccttta
     9721 ttgaccaagc ttatcatcgc agatttaacg ttgctgataa gccgggtttt ttgtagcgcc
     9781 gccatgacca ccataaaccc tgcaatcatc accacgcttg gattgacgaa tccggcaaaa
     9841 gcctgttgaa cggtcgaaag tcccgataca accagcaata aacacgcaag cagtggcggt
     9901 gcgccaaaag gcattttgtc cgacatgatc agcacgatca tcaggaccag tatgcctaat
     9961 gccaggatca tttccattgt cattttttct ctccttttag gcttccagca tgtcgcgcgg
    10021 tactttaaag accgctttgc gtattgaagt agccgtgcca attgcgcaca gcgcctggtg
    10081 aggttcctca ggcataaaga cggcgaaatc accggcgtgt agggtgatgg cgacactgtt
    10141 ttcaggggat ggggcgataa acagatcggg cttacgttct tcatcgtttt cgcgagcgat
    10201 gggccgcgtt cccgcattaa tgccctcaca accagtaatc accacctgta tatcggccca
    10261 taaatggtga tactccgtat ggcggagttc ggcgggttgc gtgtctgaag ttcccagcgt
    10321 gcagaaccac cgacatcctt caggttgcca gcgaccgtcc tctctggtgg ataacgccga
    10381 taacgaacac tccgggcgca gcagaatatt tctcacccag gcaggaagcc ccgccagcga
    10441 caaatggttg agattaccga taatcatgat gcctgcccct taacccactc gggggagatg
    10501 cgtacgtact tgatggcctg acgccaccag gtgtaagcga tatgtagcgt accgtccggg
    10561 ctttgtttga tgctggggta ggagaactca cgatttagct tttccaggga attgttggtc
    10621 atgcagtagc cgtcgccttc atcgagattg cgccgccacg gccagctttt cccaccatcg
    10681 gcagaaatcg ctaccgtcat cggcgcgcgt ggggcccccc agaaagtgga gcgtccgacg
    10741 gctgtcggtt ctttgcggct gtcgtcgcca tcgtcgattt cgtcataaag cgacgcgcga
    10801 cgttctactg cgcctgccgc actcatcgcg ttgtacacca gcgccagttc gccgctggca
    10861 agtgtcgtta cctgaataga cgaattgttg ttgggcaatt cggttgcttg cggtactgac
    10921 cagctctcgc cgttgtccgc ggaatggctg atatagatgt tgtctgccca acggctgcga
    10981 aagagagcaa ccaacgtacc gttgtgcagc attgtgatat tcatatgaac acagccaaga
    11041 ctttgcggca cctcgacatc gcgccaggag tgaccaccgt cagcggaaat tttcactgcg
    11101 ctgatatcat cattgccgac ccatttttcg ccgggttggg tacggcaata aaagacgggc
    11161 agcagccagt tgccgttatc aagcaccgtg attggctgtc gaatgaacgt accgggttta
    11221 tcaagcaggg tggcaatctc gccccaggtt tgtccgaggt cgtctgactt acggtagcgg
    11281 acgatggcgg tgtcctgatt accggatatt tgtgcggtcc ataggagcca cagcacgtta
    11341 tccggcgcga gaaacagcac cgggttttgc tctgaacgcg tgtcatcgtg ggaaagtttg
    11401 accgcatcgc tccactgcat tccgccagcg ggcaatcgcg atccccagac agaaatatcg
    11461 gcaatacctt cctgcgttcc cgcaaaccag acgcacatta acgcgccatc cggcaaaggc
    11521 agaatattcg ccgcgtggtt ttgcgggcag gacgagggca gcatggcggt aagcacatgc
    11581 gaatcgtttt cggatggacg gatgacgccg tcacgaataa gggctacaga catagttaga
    11641 ctccatctta aagttggtaa tagatttatt aatgcgagtg cgattcatgc gtttcgatca
    11701 atgtcgaaac ttttttattt cttctttcac ggataatatt gagtgtctca acacttaatg
    11761 agaaaaatat cgcgaagtag agatactctt tggcaatatg aatgttgaga ctatcggcaa
    11821 ccagcaatac accgacaaac aataaaaagg ttaacgccag tatttttatt gacggcgtgg
    11881 cgttaacgaa atcaccaatt gttttcgccg caaacatcat gacaccgacg gcaatcatta
    11941 cggcggcaat catgataaat atatgctgag aaaggccgac ggcggtgatc accgaatcca
    12001 ggctaaacac gatatcgagc agcattatct gtacaatggc accgcacaac gacagttgcc
    12061 gcgatccgct gccatgttcg tcttcctgcg cgggcgacag ctcttcgcgt atctcctgta
    12121 tcgctttata aatcaggaac agcccgcccc cgagcagaat caacgtgcgg aacgaaatct
    12181 ccagcgtcag cacggtaaaa agcggctggg taatatgtga aagccacgca atggatatca
    12241 gcaataatat ccgcataatc atcgcgcagc ttaaacccag tttacgcgcc atattttgtt
    12301 gtgataacgg aagttttgcc accaccagtg aaagaaaaat aatattatcc accccaagaa
    12361 taatttccag aaaggtgagg gtaattaaag tgagccagat atctggctgc ataaaccaca
    12421 aaaacatggg gtgatccttt attctcttta tctaaacttt atagcgggct ggataataga
    12481 gttaatataa ttaatgttat aatgacgaat aatttgttga atctcgctga tctgttcagg
    12541 cgtgaaatta accgcgtagc ggctatcacc aacatcatgc cccataagct gcaacgcttt
    12601 tttcaccgca gccggggaga atgctacttt ataaagatcg gtacgcaggc ccgttacact
    12661 ctcctgtgct tcataagacc cttcaatatc actggcatgg aagcgagacc agatggcatt
    12721 gatttctcgc ggcgcgatgt tggcgagccc agagatacag gctgaacatc cctcgacaaa
    12781 ccctttatgg atcagcgaat ccgggccgtt gagcacatta aagccgtcaa tgtcgcgcac
    12841 cgcatcgaga aaacccttca ggctttcgtc gcttccggcg ctgtctttaa tcccaatgat
    12901 attgggggtg cgacgccaga gcacgcgcgg tttctggctg gatcgtattg ccagtgcggg
    12961 ccgggatgtt atagagaaac atcggaatgc tcagtgcgtc ggcaattgcg ctgtaatgcg
    13021 caatcaactc ttcctgtttc agcggtacga agtaaggggc aatgaccgac acggcatcta
    13081 cgccaagttt ttcaatctgc tgacccagac gaatcgtttc gcgcgtagag atctcgccaa
    13141 tgtgtgccac gaccggcgca cgaccagcca cttcatccac gcaggtttgc gtaacgctga
    13201 gcttctcctg ttcattcaac acaaaaaatt cgccgttggt gccgccgcaa aaaatcgcgt
    13261 tacccgcttc cagttgacga ttcacctgca cttttaacgc cgggatattc agactgccgg
    13321 tcgcggtaaa cggggtgacg atagcggtta atacaccttc aatggttttc ggcatgattt
    13381 attccttaaa ttgatgttgc ggaaacagtg tttggtaaat ggcctgtacg tcctgtagat
    13441 caagctggca cggcacattg ttcataaggc gcttcacgtt cagtgccgcc tcgctcagag
    13501 aggcaatatc ctctggcggt acgccgagag ccgcgagatt gttgggtagc ttgagttgat
    13561 tgactaatgc ctgtaaccag gtcaccaggg catgagattt ttcttccgcg ctcagggcgg
    13621 tttccgcatc gggaatgcaa tcccagaccc gggcaaattt ctcgaccgcc caggggcgaa
    13681 caaacgccat gcacggcgcc agcaagatgg cattcgcgac gccatgcggc agatgatatt
    13741 tgccacctaa cgggtaggag agcgcatgaa cgagatgcgt gcccgcatgg gttatcgcta
    13801 cgccgccata gtaagacgcc cacagcattt ccagttttgc gcgcagatcc tgaggatcgt
    13861 tcacggcggg ttgaatgtgc cggaaaagtt tacttaaccc tgtcagcgcc gcgttatcgc
    13921 tgaccggatt tgccacggtc gcggtaaaac actccagtaa gtggcaaaga gcatcaatgc
    13981 ccgtggacgc cgcaatatgc gcgggcatgc tggtggtcag ttccgggaaa agcgccacat
    14041 agtctggtaa cagcacctgg gaaataatac ccaccttcgt gctttgctca gggattgcca
    14101 gaatcgcatt cggcgtggct tctgagccgg ttccggcggt tgtaggaatc aaccatgatt
    14161 gcacccgctg agtcggtttt tcacccgcaa gcagcgcatc cagccccgga gattgtggat
    14221 ggcaaagcac cgatagcagc tttgccacat ccaacacgct gccaccgccg atcccgacca
    14281 ccacatcaaa agaggcatcg ccaaggacat ccattagctg gcgcacatca tgatgcgtgg
    14341 gctctgcggg cacattatcg ataacgttaa cctgcgggca gtgcttttcc agtaaggcgc
    14401 gaatctgctg cacaccgtcg agctgcgcaa tattacggtc agtgaccagc agcatttttc
    14461 tgatgtcggg taagagcgac gtcagggaag ggatagcgcc tgcgccgctc agtatcgtgc
    14521 tattgataat taccacaaat aacctctctt ctggtgattg aattcaatca ttcaaaatgc
    14581 cgtagcctac gtaacatggt ctaattttat aatttattcg agtgattaat aacttgagct
    14641 tgctcacatt taatcaattg tgattgaata taatcattgt tatcataaaa caacctggag
    14701 tgatgatgga aaagggaatc actaagccga gcattctcgt cgttgccgat gatttcacgg
    14761 gagccaacga cgcgggggta agcctggcgc aggtggggca tacagttgat gtggcctttg
    14821 aaatgcatta tcgcggtgat gccagcgtat gggtgattaa cagcgacagc cgggcgatgg
    14881 atcctaagct ggcggcgatg aaaataacct cgctgatgag tcatctccct ctggcgaata
    14941 atccgccgct cgtgattaaa aagatagatt ccacgctgcg aggcaatatc ggcgcagaaa
    15001 ttgaagccct gatgaaggca tgcggtataa cgggcgcagt ggttgccccc gcatttcctc
    15061 aggcgggaag gaccacagtg gccggtgagt gttgggtaaa cggtgtgcgg ataacggaaa
    15121 ccgagtttgc cagcgatccg aaaaccccgg tattgagcgc ccgaattgcg gacatcatcc
    15181 ggttgcagac ggccattcca tgccagcccg ttacggtttc tcagctcagc catttgtctt
    15241 atgagcaacc gtggattagc gtgatagatg cgcagacgga cagcgacctg gatcgcatcg
    15301 ctgccgctgt gatgcaggct aaacaaccgc tgctgctggt cggctccgct ggtatttgtg
    15361 atgccgttgc ccggcgctcc gcgattatgc cgccacccac cgtgctggcc attattggtt
    15421 caatgagcga aatcgctcaa cggcagatcg ccacacttca ttcacaccca cgtatcacac
    15481 agatatacgt cgatgttgaa cacattctgg caggcaatgc cagtgactat gacgcgcgta
    15541 ttgtgcaggc cttgcagaaa ggcgaccact gcattgtcca cacctgtaac gattctgtag
    15601 caaggcatca gatcgacacc ctttgccagc gttggcagat gagtcgcgct gcacttgggg
    15661 aaaaaatttg ccggtttctt ggcgaactta cccggcaagt tctgcttcgc acaatgcccg
    15721 atgcgcttta tctctctggt ggtgatgtcg caatggcaac agccagtgcg ttaggcgcga
    15781 ccggatttcg tattacgggg aaggtggcgc aatgcgttcc atacggtcac tttctgggcg
    15841 gtgtctggtc ccgttcggtg atgacgaaag ccggtgggtt tggcgatgag accacgttgc
    15901 atcaggttct gaattttatc gaggagaagt gtagtgaata atattattgc ggtaacgatg
    15961 ggtgacccgg cgggtattgg cccggagatc attattaaat ccctgagcga agccgggctt
    16021 gctggtgccc cggtggtggt ggtgggctgt gtgcgcacgc tgcgacgtat tatggcgatg
    16081 aatatcacgc cgcaggcaga gttacgcgtc attcaaaagg tgtgcgacgc ccattttgct
    16141 cccggcgtga ttaacgtgat ggatgagccg ctggctaacc ccgatgcgct gacacccgga
    16201 gtggtacagg ctgtggcagg cgatttagcg tatcgctgta tcaaacgtgc aacggctctg
    16261 gccttatcag gcgaggtgaa agccatagcc actgcgccgc tgaataaaga agcgctgcat
    16321 cttggcgggc ataactatcc ggggcatacg gaattgctgg cccatcttac cgccagcaaa
    16381 gagtacgcga tggtgctcta taccgataag ctcaaggtta tccatatttc gacacatatc
    16441 tcactgcgta aattcctgga tactttgaat ggtgaaaggg tcaaaacggt tattcgtgtt
    16501 gcgaaccact tcctgaaacg tgttggtatt gagcgtccgc gtattgccgt ggcgggtgta
    16561 aatccacatg caggggaaca tggtttattc ggcactgagg aaatcgagat tatcgcgcct
    16621 gcgattgaag caatgcaggc cgaagatatt gatgttactg gcccgtgccc gccagatacc
    16681 gtgtttatgc aatgccatga agggctattc gatatggtag tggctatgta ccacgatcag
    16741 gggcatatcc ctttaaaact gcttggattt tatgatgggg tgaatatcac cgccgggttg
    16801 ccatttattc gcacttctgc cgaccacggc accgcgtttg atattgcctg gacaggaaaa
    16861 gccaagtctg agagcatggc agtttccatc cagttagcaa tgcagatttc gcgagaataa
    16921 aatgaaggga tataaccggt tagagcagat catggattac ctgaaaagcc ataatctggt
    16981 caccgttgat gagctggtgg cggtaaccaa tgcgtcgccg gctacgattc gtcgagacct
    17041 gataaaactc gatgagcagg gtgttatcag ccgtacacac ggcggtgtga ccctcaaccg
    17101 gtttattcct acccaaccga ctacccatga gaaaatgcag cgcagcctgg cggaaaaaca
    17161 tgccatcgcc agcgcagcgg cgtcaatggt caaagcgggg gattccgtcg tccttgatgc
    17221 cggtacgaca atgattgaac ttgcgcggca gattacacat ttaccacttc gcgtgatcac
    17281 cagcgacctg catatcgcgc tttttttggc tgaattcaag caaattgaag tgacgattat
    17341 cggtggacgt atcgacgact ccagccagtc ctgcattggc gaacatggtc gtaaattgct
    17401 gcaaaatacc tggccggacg tggcattttt aagctgtaac agctgggatc ttgaaaaggg
    17461 tattacagca cccacggaag agaaagccgc actaaaacgc gatcttatcg cgcatgcttc
    17521 gcgcaaaatt ctcctggctg acagctcaaa atacggttca tggtcgctgt ttaatatcgc
    17581 gcatcttaat gaactgacag agattattac ggatagtcag cttgatgggc agacgcgtag
    17641 aacgcttgcg tccttgtcga cgcgtttaat catagccgac taatctatga ctctgcggcc
    17701 agctcatccg gcaatttaat aaatgcgtca ggctggcgac gtttggcctt acgtttccag
    17761 cgcgaggatc tgctggcagt agcgcagaaa acgccccaat atcttacgca aaaccatttt
    17821 ttctctaact aacagttgtc agcctgtccc gcttataaga tcatacgccg ttatacgttg
    17881 tttacgcttt gaggaatcca cgatgagtga ggcagaagcc cgcccgacta actttattcg
    17941 tcagatcatc gatgaagatc tggccagtgg taagcacacc acagtacaca cccgtttccc
    18001 gccggagccg aatggctatc tgcatattgg ccatgcgaaa tctatctgcc tgaacttcgg
    18061 gatcgcccag gactataaag gccagtgcaa cctgcgtttc gacgacacta acccggtaaa
    18121 agaagatatt gagtatgtcg attcgatcaa aaacgacgta gagtggttag gtttccactg
    18181 gtctggtaac gtccgttact cctccgatta ttttgatcag ctccacgcct atgcgatcga
    18241 actgatcaat aaaggcctgg cgtacgttga tgaactgacg ccggaacaga tccgcgaata
    18301 ccgcggcacc ttgacgcagc cgggtaaaaa cagcccgtac cgcgatcgca gcgttgaaga
    18361 gaacctggcg ctgttcgaga aaatgcgtac cggcggtttt gaagaaggta aagcctgcct
    18421 gcgtgcgaag atcgacatgg cttcgccgtt tatcgtgatg cgcgatccgg tgctgtaccg
    18481 cattaagttt gctgaacacc accagactgg caacaagtgg tgcatctacc cgatgtacga
    18541 cttcacccac tgcatcagcg atgcgctgga aggtattacg cactctctgt gtacgcttga
    18601 gttccaggac aaccgccgtc tgtacgactg ggtactggac aacatcacca ttcctgttca
    18661 cccgcgccag tacgaattct cgcgcctgaa tctggaatac actgtgatgt ccaagcgtaa
    18721 gctgaacctg ctggtgaccg ataagcacgt tgaaggctgg gatgacccgc gtatgccgac
    18781 catttctggt ctgcgtcgtc gtggttacac tgcggcttct attcgtgagt tctgcaaacg
    18841 cattggtgtg accaagcagg acaacaccat tgagatggcg tcactggaat cctgcatccg
    18901 tgaagatctc aacgaaaatg cgccgcgcgc aatggcggtt atcgatccgg tgaaactggt
    18961 tatcgaaaac taccagggcg aaggcgaaat ggttaccatg ccgaaccatc cgaataaacc
    19021 ggaaatgggc agccgtcagg tgccatttag cggtgagatt tggatcgatc gcgccgattt
    19081 ccgcgaagaa gctaacaagc agtacaaacg tctggtgctg ggtaaagaag tgcgtctgcg
    19141 taatgcttac gtcattaagg cagaacgcgt cgagaaagat gccgaaggca atatcaccac
    19201 catcttctgt acttatgacg ccgacacctt aagcaaagat ccggcagatg gtcgtaaagt
    19261 caaaggtgtt attcactggg tgagcgcggc acatgcgctg ccggttgaaa tccgtttgta
    19321 tgaccgtctg ttcagcgtgc cgaaccctgg tgctgcggat gatttcctgt cggtgattaa
    19381 cccggaatcg ctggtgatca aacagggctt tgctgaaccg tcgctgaaag atgcggttgc
    19441 gggtaaagca ttccagtttg agcgtgaagg ttacttctgc ctcgacagcc gccattctac
    19501 ggcggaaaaa ccggtattta accgcaccgt tgggctgcgt gatacctggg cgaaagtagg
    19561 cgagtaagtt tacgttgtcg gatagagcgt ttacgccgca tccggcaaga aatatgcaaa
    19621 ttatcccaaa cgccgcgctc tgcggcg
//
LOCUS       DADSYM010000090        19613 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2415
            SAMN08039579-rid11723173.denovo.090, whole genome shotgun sequence.
ACCESSION   DADSYM010000090 DADSYM010000000
VERSION     DADSYM010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039579
            Sequence Read Archive: SRR6362533
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 19613)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19613)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980162.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 03:39:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,484
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 4,347
            CDSs (with protein)               :: 4,347
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 43 of 85
            Pseudo Genes (incomplete)         :: 37 of 85
            Pseudo Genes (internal stop)      :: 19 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19613
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039579-rid11723173.denovo.090"
                     /strain="LGE2415"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8302 N 95.9628 W"
                     /collection_date="2015-08-24"
     gene            <1..3038
                     /locus_tag="J1Y83_002755"
     CDS             <1..3038
                     /locus_tag="J1Y83_002755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="adhesin"
                     /protein_id="HBA7630889.1"
                     /translation="NIATNTTDIATNTADIATNASSITTLNADVDTLEKDALLWNGTA
                     FSAKHGTEATSKITNVTAGDLTAGSTDAVNGSQLKTTNDNVSTNTTNITNLTDSVGDL
                     KDDSLLWNKAAGAFSAAHGTDTTSKITNVRAGSVSKNSTDAINGSQLYGVADSFTSYL
                     GGGADISDTGVLTGPTYTIGDTDYTNVGDALAAINTSFSSSLGDALLWDATAGKFSAK
                     HGINNDPSVITDVANGAVSSTSSDAINGSQLYGVSDYIADALGGNAVVNADGSITTPT
                     YAIAGGSYDNVGDALEAIDTTLDDALLWDETANGGNGAFSAAHGKDKTASVITNVANG
                     AVSATSSDAINGSQLYSTNKYIADALGGDAEVNADGTITAPTYTIANTDYNNVGEALD
                     ALDNNALLWDATANNGAGAYSASHDGKASIITNVADGNIGEDSTDAINGSQLNATNMM
                     IQQNSEIINQLAGDTSKNYIGDNGAGINYVRTNDSGLAFNDASAAGIGSTAVGYNTVA
                     KGDSSVAIGHGSNSIVETSIALGSESVSSRLIVKGSRNTRVTENGVEIGYDTTDGELL
                     GALSIGDDGKYRQIINVADGSEAHDAVTVRQLQNAIGAVATTPTKYYHANSTAEDSLA
                     VGEDSLAMGAKTIVNGNAGIGIGLNTLVLADAINGIAIGSNARANHADSIAMGNGSQT
                     TRGAQTNYTAYNMDAPQNSVGEFSVGSEDGQRQITNVAAGSADTDAVNVGQLKVTDAQ
                     VSQNTQSITNLNTQVTNLDTRVTNIENGIGDIVTTGSTKYFKTNTDGADANAQGKDSV
                     AIGSGSIAAADNSVALGTGSVADEENTISVGSSTNQRRITNVAAGVNATDAVNVSQLK
                     SSEAGGVRYDTKADGSIDYSNITLGGGNGGTTRISNVSAGVNNNDAVNYAQLKQSVQE
                     TKQYTDQRMVEMDNKLSKTESKLSGGIASAMAMTGLPQAYTPGASMASIGGGTYNGES
                     AVALGVSMVSANGRWVYKLQGSTNSQGEYSAALGAGIQW"
     gene            3450..5105
                     /gene="lldP"
                     /locus_tag="J1Y83_002756"
     CDS             3450..5105
                     /gene="lldP"
                     /locus_tag="J1Y83_002756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate permease"
                     /protein_id="HBA7630890.1"
                     /translation="MNLWQQNYDPAGNIWLSSLIASLPILFFFFALIKLKLKGYVAAS
                     WTVAIALAVALLFYKMPVANALASVVYGFFYGLWPIAWIIIAAVFVYKISVKTGQFDI
                     IRSSILSITPDQRLQMLIVGFCFGAFLEGAAGFGAPVAITAALLVGLGFKPLYAAGLC
                     LIVNTAPVAFGAMGIPILVAGQVTGIDSFEIGQMVGRQLPFMTIIVLFWIMAIMDGWR
                     GIKETWPAVVVAGGSFAIAQYLSSNFIGPELPDIISSLVSLLCLTLFLKRWQPVRVFR
                     FGDLGASQVDMTLAHTGYTAGQVLRAWTPFLFLTATVTLWSIPPFKALFASGGALYEW
                     VINIPVPYLDKLVARMPPVVSEATAYAAVFKFDWFSATGTAILFAALLSIVWLKMKPS
                     DAISTFSSTLKELALPIYSIGMVLAFAFISNYSGLSSTLALALAHTGHAFTFFSPFLG
                     WLGVFLTGSDTSSNALFAALQATAAQQIGVSDLLLVAANTTGGVTGKMISPQSIAIAC
                     AAVGLVGKESDLFRFTVKHSLIFTCMVGVITTLQAYVLTWMIP"
     gene            5105..5881
                     /gene="lldR"
                     /locus_tag="J1Y83_002757"
     CDS             5105..5881
                     /gene="lldR"
                     /locus_tag="J1Y83_002757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LldR"
                     /protein_id="HBA7630891.1"
                     /translation="MIVLPRRLSDEVADRVRALIDEKNLEAGMKLPAERQLAMQLGVS
                     RNSLREALAKLVSEGVLLSRRGGGTFIRWRHDTWSEQNIVQPLKTLMADDPDYSFDIL
                     EARYAIEASTAWHAAMRATPGEKEKIQLCFEATLSEDPDLASQADVRFHLAIAEASHN
                     IVLLQTMRGFFDVLQSSVKHSRQRMYLVPPVFSQLTEQHQAVIDAIFAGDADGARKAM
                     MAHLSFVHTTMKRFDEDQARHARITRLPGDHNEHSREKNA"
     gene            5878..7068
                     /gene="lldD"
                     /locus_tag="J1Y83_002758"
     CDS             5878..7068
                     /gene="lldD"
                     /locus_tag="J1Y83_002758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent L-lactate dehydrogenase"
                     /protein_id="HBA7630892.1"
                     /translation="MIISAASDYRAAAQRILPPFLFHYMDGGAYSEYTLRRNVEDLSE
                     VALRQRILKNMSDLSLETTLFNEKLSMPVALGPVGLCGMYARRGEVQAAKAADAHGIP
                     FTLSTVSVCPIEEVAPAIKRPMWFQLYVLRDRGFMRNALERAKAAGCSTLVFTVDMPT
                     PGARYRDAHSGMSGPNAAMRRYLQAVTHPQWAWDVGLNGRPHDLGNISAYLGKPTGLE
                     DYIGWLGNNFDPSISWKDLEWIRDFWDGPMVIKGILDPEDARDAVRFGADGIVVSNHG
                     GRQLDGVLSSARALPAIADAVKGDIAILADSGIRNGLDVVRMIALGADTVLLGRAFLY
                     ALATAGQAGVANLLNLIEKEMKVSMTLTGAKSISEITQDSLVQELSKAPAAALAPMAK
                     GNAA"
     gene            7116..7589
                     /gene="trmL"
                     /locus_tag="J1Y83_002759"
     CDS             7116..7589
                     /gene="trmL"
                     /locus_tag="J1Y83_002759"
                     /EC_number="2.1.1.-"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HBA7630893.1"
                     /translation="MLNIVLYEPEIPPNTGNIIRLCANTGFRLHIIEPMGFAWDDKRL
                     RRAGLDYHEFTAVTRHHDYRAFLEAENPQRLFALTTKGTPAHSAVSYQDGDYLMFGPE
                     TRGLPASILDALPAEQKIRIPMVPDSRSMNLSNAVSVVVYEAWRQLGYPGAVLRD"
     gene            complement(7642..8463)
                     /gene="cysE"
                     /locus_tag="J1Y83_002760"
     CDS             complement(7642..8463)
                     /gene="cysE"
                     /locus_tag="J1Y83_002760"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001112113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HBA7630894.1"
                     /translation="MSCEELEIVWNNIKAEARTLADCEPMLASFYHATLLKHENLGSA
                     LSYMLANKLSSPIMPAIAIREVVEEAYAADPEMIASAACDIQAVRTRDPAVDKYSTPL
                     LYLKGFHALQAYRIGHWLWNQGRRALAIFLQNQVSVTFQVDIHPAAKIGRGIMLDHAT
                     GIVVGETAVIENDVSILQSVTLGGTGKSGGDRHPKIREGVMIGAGAKILGNIEVGRGA
                     KIGAGSVVLQPVPPHTTAAGVPARIVGKPDSDKPSMDMDQHFNGINHTFEYGDGI"
     gene            complement(8543..9562)
                     /gene="gpsA"
                     /locus_tag="J1Y83_002761"
     CDS             complement(8543..9562)
                     /gene="gpsA"
                     /locus_tag="J1Y83_002761"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HBA7630895.1"
                     /translation="MNQRNASMTVIGAGSYGTALAITLARNGHEVVLWGHDPEHIATL
                     ERDRCNAAFLPDVPFPDTLHLESDLATALAASRNILVVVPSHVFGEVLRQIKPLMRPD
                     ARLVWATKGLEAETGRLLQDVAREALGDQIPLAVISGPTFAKELAAGLPTAISLASTD
                     QTFADDLQQLLHCGKSFRVYSNPDFIGVQLGGAVKNVIAIGAGMSDGIGFGANARTAL
                     ITRGLAEMSRLGAALGADPATFMGMAGLGDLVLTCTDNQSRNRRFGMMLGQGMDVQSA
                     QEKIGQVVEGYRNTKEVRELAHRFGVEMPITEEIYQVLYCGKNAREAALTLLGRARKD
                     ERSSH"
     gene            complement(9562..10029)
                     /gene="secB"
                     /locus_tag="J1Y83_002762"
     CDS             complement(9562..10029)
                     /gene="secB"
                     /locus_tag="J1Y83_002762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-export chaperone SecB"
                     /protein_id="HBA7630896.1"
                     /translation="MSEQNNTEMTFQIQRIYTKDISFEAPNAPHVFQKDWQPEVKLDL
                     DTASTQLADDVYEVVLRVTVTASLGEETAFLCEVQQGGIFSIAGIEGTQMAHCLGAYC
                     PNILFPYARECITSMVSRGTFPQLNLAPVNFDALFMNYLQQQAGEGTEEHQDA"
     gene            complement(10093..10344)
                     /gene="grxC"
                     /locus_tag="J1Y83_002763"
     CDS             complement(10093..10344)
                     /gene="grxC"
                     /locus_tag="J1Y83_002763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 3"
                     /protein_id="HBA7630897.1"
                     /translation="MANVEIYTKETCPYCHRAKALLSSKGVSFQELPIDGNAAKREEM
                     IKRSGRTTVPQIFIDAQHIGGCDDLYALDARGGLDPLLK"
     gene            complement(10485..10916)
                     /locus_tag="J1Y83_002764"
     CDS             complement(10485..10916)
                     /locus_tag="J1Y83_002764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HBA7630898.1"
                     /translation="MQEIMQFVGRHPILSIAWIALLVAVLVTTFKSLTSKVKVITRGE
                     ATRLINKEDAVVVDLRQRDDFRKGHIAGSINLLPSEIKANNVGELEKHKDKPVIVVDG
                     SGMQCQEPANALTKAGFAQVFVLKEGVAGWAGENLPLVRGK"
     gene            11161..12705
                     /gene="gpmM"
                     /locus_tag="J1Y83_002765"
                     /gene_synonym="pgmI"
     CDS             11161..12705
                     /gene="gpmM"
                     /locus_tag="J1Y83_002765"
                     /gene_synonym="pgmI"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HBA7630899.1"
                     /translation="MSVSKKPMVLVILDGYGYREEQQDNAIFSAKTPVMDALWANRPH
                     TLIDASGLEVGLPDRQMGNSEVGHVNLGAGRIVYQDLTRLDVEIKDRAFFANPVLTGA
                     VDKAKNAGKAVHIMGLLSAGGVHSHEDHIMAMVELAAERGAEKIYLHAFLDGRDTPPR
                     SAESSLKKFEEKFAALGKGRVASIIGRYYAMDRDNRWDRVEKAYDLLTLAQGEFQADT
                     AVAGLQAAYARDENDEFVKATVIRAEGQPDAAMEDGDALIFMNFRADRAREITRAFVN
                     ADFDGFARKKVVNVDFVMLTEYAADIKTAVAYPPASLVNTFGEWMAKNDKTQLRISET
                     EKYAHVTFFFNGGVEESFKGEDRILINSPKVATYDLQPEMSSAELTEKLVAAIKSGKY
                     DTIICNYPNGDMVGHTGVMEAAVKAVEALDHCVEEVAKAVESVGGQLLITADHGNAEQ
                     MRDPATGQAHTAHTNLPVPLIYVGDKNVKAVEGGKLSDIAPTMLSLMGMEIPQEMTGK
                     PLFIVE"
     gene            12715..13998
                     /gene="envC"
                     /locus_tag="J1Y83_002766"
     CDS             12715..13998
                     /gene="envC"
                     /locus_tag="J1Y83_002766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein hydrolase activator EnvC"
                     /protein_id="HBA7630900.1"
                     /translation="MRGKAINTMTRAVKPRRFAIRPIIYASVLSAGVLLCAFSAHADE
                     RDQLKSIQADIAAKERAVRQKQQQRASLLAQLKKQEEAISEATRKLRETQNTLNQLNK
                     QIDEMNASIAKLEQQKAAQERSLAAQLDAAFRQGEHTGIQLILSGEESQRGQRLQAYF
                     GYLNQARQETIAQLKQTREEVAMQRAELEEKQSEQQTLLYEQRAQQAKLTQALSERKK
                     TLAGLESSIQQGQQQLSELRANESRLRNSIARAEAAAKARAEREAREAQAVRDRQKEA
                     TRKGTTYKPTESEKSLMSRTGGLGAPRGQAFWPVRGPTLHRYGEQLQGELRWKGMVIG
                     ASEGTEVKAIADGRVILADWLQGYGLVVVVEHGKGDMSLYGYNQSALVSVGSQVRAGQ
                     PIALVGSSGGQGRPSLYFEIRRQGQAVNPQPWLGR"
     gene            14002..14961
                     /locus_tag="J1Y83_002767"
     CDS             14002..14961
                     /locus_tag="J1Y83_002767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312519.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divergent polysaccharide deacetylase family
                     protein"
                     /protein_id="HBA7630901.1"
                     /translation="MFPFRRNVLAFAALLALSSPVIAGKLAIVIDDFGYRPHNENQVL
                     AMPSAISVAVLPDSPHAREMATKAHNSGHEVLIHLPMAPLSKQPLEKNTLRPEMSSDE
                     IERIIRSAVNNVPYAVGINNHMGSKMTSNLFGMQKVMQALERYNLYFLDSVTIGNTQA
                     MRAAQGTGVKVIKRKVFLDDSQNEADIRVQFNRAIDLARRNGSTIAIGHPHPSTVRVL
                     QQMVYNLPPDITLVKASSLLNEPQVDTSTPPKNTVPDTPRNPFHGVKLCKPKKPIEPV
                     YANRFFEVLSESISQSTLIVYFQHQWQGWGKQPESAKLNASAN"
     gene            complement(14948..15982)
                     /gene="waaH"
                     /locus_tag="J1Y83_002768"
     CDS             complement(14948..15982)
                     /gene="waaH"
                     /locus_tag="J1Y83_002768"
                     /EC_number="2.4.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucuronate:LPS(HepIII) glycosyltransferase"
                     /protein_id="HBA7630902.1"
                     /translation="MMNSTNKLSVIIPLYNAGDDFRTCMESLITQTWTALEIIIINDG
                     STDNSVEIAKHYAENYPHVRLLHQANAGASVARNRGIEVATGKYVAFVDADDEVYPTM
                     YETLMTMALEDDLDVAQCNADWCFRETGETWQSIPTDRLRSTGVLTGPDWLRMGLSSR
                     RWTHVVWMGVYRRDVIVKNNIKFIAGLHHQDIVWTTEFMFNALRARYTEQSLYKYYLH
                     NTSVSRLNRQGNKNLNYQRHYIKITRLLEKLNRNYADKITIYPEFHQQITYEALRVCH
                     AVRKEPDILTRQRMIAEIFTSGMYKRLITNVRSVKVGYQALLWSFRLWQWRDKTRSHH
                     RITRSAFNLR"
     gene            complement(16221..17246)
                     /gene="tdh"
                     /locus_tag="J1Y83_002769"
     CDS             complement(16221..17246)
                     /gene="tdh"
                     /locus_tag="J1Y83_002769"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HBA7630903.1"
                     /translation="MKALSKLKAEEGIWMTDVPVPELGHNDLLIKIRKTAICGTDVHI
                     YNWDEWSQKTIPVPMVVGHEYVGEVVGIGQEVKGFKIGDRVSGEGHITCGHCRNCRGG
                     RTHLCRNTIGVGVNRPGCFAEYLVIPAFNAFKIPDNISDDLASIFDPFGNAVHTALSF
                     DLVGEDVLVSGAGPIGIMAAAVAKHVGARNVVITDVNEYRLELARKMGITRAVNVAKE
                     NLNDVMTELGMTEGFDVGLEMSGAPPAFRTMLDTMNHGGRIAMLGIPPSDMSIDWTKV
                     IFKGLFIKGIYGREMFETWYKMAALIQSGLDLSPIITHRFSIDDFQKGFDAMRSGQSG
                     KVILSWD"
     gene            complement(17256..18452)
                     /gene="kbl"
                     /locus_tag="J1Y83_002770"
     CDS             complement(17256..18452)
                     /gene="kbl"
                     /locus_tag="J1Y83_002770"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HBA7630904.1"
                     /translation="MRGEFYQQLTNDLETARAEGLFKEERIITSAQQADITVADGSHV
                     INFCANNYLGLANHPDLIAAAKAGMDSHGFGMASVRFICGTQDSHKELEQKLAAFLGM
                     EDAILYSSCFDANGGLFETLLSAEDAIISDALNHASIIDGVRLCKAKRYRYANNDMHE
                     LEARLKEAREAGARHVLIATDGVFSMDGVIANLKGVCDLADKYDALVMVDDSHAVGFV
                     GENGRGSHEYCDVMGRVDIITGTLGKALGGASGGYTAARKEVVEWLRQRSRPYLFSNS
                     LAPAIVAASIKVLEMVEAGSELRDRLWANARQFREQMSAAGFTLAGADHAIIPVMLGD
                     AVVAQKFARELQKEGIYVTGFFYPVVPKGQARIRTQMSAAHTPEQITRAVEAFTRIGK
                     QLGVIA"
     gene            complement(18727..19584)
                     /gene="yibB"
                     /locus_tag="J1Y83_002771"
     CDS             complement(18727..19584)
                     /gene="yibB"
                     /locus_tag="J1Y83_002771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YibB"
                     /protein_id="HBA7630905.1"
                     /translation="MKSSTTIITAYFDIGRGDWTANKGFREKLARSVDVYFSYFERLA
                     ALENEMIIFTSPDLKSRVEAIRNGKPTTVIVIDIKKKFRYIRSRIEKIQKDESFTNRL
                     EPRQLKNPEYWSPEYVLVCNLKAYFVNKAINMGLVKTPLVAWIDFGYCRKPNVTRGLK
                     IWDFPFDENKMHLFTIKKGLAVTSQQQAFDFMIGNHVYIIGGAIVGSQHKWKEFYKLV
                     LESQKITLNNNIVDDDQGIFVMCYYKRSDLFNLNYLGRGKWFDLFRCFRSNTLGAKMQ
                     ALRIFLSRK"
BASE COUNT         4867 a         5166 c         4994 g         4586 t
ORIGIN      
        1 ccaatatcgc cactaacact acggatattg cgactaatac cgccgatata gctacaaacg
       61 ctagcagtat cactactctg aacgcggacg ttgataccct ggaaaaagat gccctgctct
      121 ggaatggcac agcattcagc gccaaacacg gcaccgaagc taccagcaag atcaccaatg
      181 ttaccgctgg cgacctgact gctggcagca ctgacgccgt taatggctcc cagctcaaaa
      241 ccaccaacga taacgtgtcg accaacacca ccaacatcac caacctgacg gattccgttg
      301 gcgaccttaa ggacgattcc ctgctgtgga acaaagcggc tggcgcattc agcgccgcgc
      361 acggcactga taccaccagc aaaatcacta acgttagagc tggctcggtt tctaaaaaca
      421 gcactgacgc cattaacggc tcacaacttt atggcgtagc ggattcattt acgtcatatc
      481 ttggtggtgg tgctgatatc agcgatacgg gtgtattaac cgggccaacc tacactattg
      541 gtgatactga ctacactaac gtcggtgatg ctctggcagc cattaacaca tcatttagct
      601 catcactcgg cgacgcccta ctctgggatg caaccgcagg taaattcagc gccaaacacg
      661 gcattaataa tgatcccagt gtaatcactg atgttgcaaa cggtgcagtc tcgtccacca
      721 gtagcgacgc cattaacggt tcgcaacttt atggcgttag tgactacatt gccgatgctt
      781 tgggcgggaa cgctgtggtg aatgcagacg gcagtatcac tacaccgact tatgccatag
      841 cgggcggcag ttacgacaat gtcggtgacg cgctggaagc gatcgatacc acgctggatg
      901 acgccctgct gtgggatgag acagccaatg gcggtaacgg tgcatttagc gccgctcacg
      961 ggaaagataa aactgccagt gtaatcacta acgtcgctaa cggtgcagtc tctgccacca
     1021 gcagcgatgc cattaatggc tcacagctct atagcaccaa taagtacatc gctgatgcgc
     1081 tgggtggtga tgcagaagtc aacgctgacg gaactatcac tgcaccgact tacaccattg
     1141 caaataccga ttacaacaac gtcggtgaag ccctggatgc gcttgataat aacgctctgc
     1201 tgtgggatgc aacagccaat aacggcgcag gtgcttacag cgccagtcat gacggcaagg
     1261 ccagcattat cacaaacgtt gctgatggta acattggtga agatagcacc gacgctatca
     1321 acggttctca gttgaatgca accaacatga tgatccagca gaacagcgaa atcatcaatc
     1381 agcttgccgg tgacaccagt aaaaactaca tcggagataa cggtgcaggt attaactatg
     1441 tacgtaccaa cgacagcggc ttagcgttca acgatgccag cgcagcaggt attggctcta
     1501 ccgctgtggg gtataacact gttgccaaag gcgatagcag cgtggcaatc ggtcatggca
     1561 gcaacagcat cgttgagacg agtattgcgc tgggtagcga atccgtctcc agccgtttga
     1621 ttgtgaaagg ctcccgtaat acccgcgtta cggaaaatgg ggtcgagatc ggttatgaca
     1681 ccacggatgg cgaactgctt ggcgcgttgt cgatcggtga tgatggtaaa tatcgtcaaa
     1741 tcatcaacgt cgcggatggt tctgaagccc atgacgctgt tactgttcgc cagttgcaaa
     1801 acgccattgg tgcagtagca accacaccaa ccaaatacta tcacgccaac tcaacggctg
     1861 aagactcact ggcagtcggt gaagactcgc tggcaatggg cgcgaaaacc atcgttaatg
     1921 gtaatgcggg tattggtatc ggcctgaaca ctttagttct ggctgatgcg atcaacggta
     1981 ttgctatcgg ttctaacgca cgcgcaaatc atgccgacag cattgctatg ggtaatggtt
     2041 ctcagactac ccgtggtgcg cagaccaact acactgccta caacatggac gcaccgcaga
     2101 actctgtggg tgagttctct gtcggcagcg aagacggtca acgtcagatc accaacgtcg
     2161 cagcaggttc ggcggatacc gatgcggtta acgtgggtca gttgaaagta acggacgcgc
     2221 aggtttccca gaatacccag agcattacta acctgaacac tcaggtcact aatctggata
     2281 ctcgcgtgac caatatcgaa aacggcattg gcgatatcgt aaccaccggt agcactaagt
     2341 acttcaagac caacaccgat ggcgcagatg ccaacgcgca gggtaaagac agtgttgcga
     2401 ttggttctgg ttccattgct gccgctgaca acagcgtcgc actgggcacg ggttccgtag
     2461 cagacgaaga aaatacgatc tctgtgggtt cttctaccaa ccagcgccgt atcaccaacg
     2521 ttgctgccgg tgttaatgcc accgatgcgg ttaacgtttc gcaactgaag tcttctgaag
     2581 caggcggcgt gcgctacgac accaaagctg atggctctat cgactacagc aacatcactc
     2641 tcggtggtgg taacggcggt acgactcgca tcagcaacgt ttccgctggc gtgaataaca
     2701 acgacgcagt gaactatgcg cagttgaagc aaagtgtaca ggaaacgaag caatacaccg
     2761 atcagcgcat ggttgagatg gataacaaac tgtccaaaac tgaaagcaag ctgagtggtg
     2821 gtatcgcttc tgcaatggca atgaccggtc tgccgcaggc ttacacgccg ggtgccagca
     2881 tggcgtctat tggtggcggt acttacaacg gtgaatcggc tgttgcttta ggtgtgtcga
     2941 tggtgagcgc caatggtcgt tgggtctaca aattacaagg tagtaccaat agccagggtg
     3001 aatactccgc cgcactcggt gccggtattc agtggtaatg atccattaac aaatgggtaa
     3061 acgccacatt gcctgatgcg ctacgcttat caggcctacg gggtgattca gcacattcct
     3121 gtaggccgga taaggcgttc acgccgcatc cggcaagata aggcgcacca gatcaacaat
     3181 ctgagggcaa ttctctgatg ggaattgccc ttttcttcat tacctctctc ccaggattgg
     3241 ccctaccaat tcttcattga tctgacctct ggttcacaat tccccaatta aaactcacat
     3301 caccgttgcc aataaataac atttagttaa ctattcattg tcattatccc tacacaacac
     3361 aattggcagt gccactttta cacaacgtgt gacaaggaga tgagcaacag actcattaca
     3421 cggcgtgtgt ggactgcagg agacctgcaa tgaatctctg gcaacaaaac tacgatcccg
     3481 ccgggaatat ctggctttcc agcctgatag catcgcttcc catcctgttt ttcttctttg
     3541 cgctgattaa gctcaaactg aaaggatacg tcgccgcctc atggacggtg gcaatcgccc
     3601 ttgccgtggc tttgctgttc tataaaatgc cggtcgctaa cgcgctggcc tcggtggttt
     3661 atggcttctt ctacgggttg tggcccatcg cgtggatcat tattgcagcg gtgttcgtct
     3721 ataagatctc ggtgaaaacc gggcagtttg acatcatccg ctcgtctatt ctttcgataa
     3781 cccctgacca gcgcctgcaa atgctgatcg tcggtttctg tttcggcgct ttccttgaag
     3841 gagccgcagg ctttggcgca ccggtagcaa ttaccgccgc attgctggtc ggtctgggct
     3901 ttaaaccact gtacgccgcc gggctgtgcc tgattgttaa caccgcgcca gtggcatttg
     3961 gtgcgatggg cattccaatc ctggttgccg gccaggtaac aggcatcgac agctttgaga
     4021 ttggtcagat ggtggggcgt cagctaccgt ttatgaccat tatcgtgctg ttctggatca
     4081 tggcgattat ggacggctgg cgcggtatca aagaaacctg gcctgcggtc gtggttgcgg
     4141 gcgggtcgtt tgccattgct caatacctca gctctaactt tattgggccg gaactgccag
     4201 acattatctc ttcgctggta tcgctgctct gtctgacact gttcctcaaa cgctggcaac
     4261 cagtgcgcgt attccgcttc ggtgacttag gggcgtcaca ggttgatatg acgctggctc
     4321 acacaggtta cacggcaggt caggtgctgc gcgcctggac accgttcctg ttcctgaccg
     4381 ccaccgtaac gctgtggagt atcccgccgt ttaaagccct gttcgcttca ggcggtgcgc
     4441 tgtatgagtg ggtgatcaac attccggtgc cgtacctcga taaactggtt gcccgtatgc
     4501 cgccagtggt cagcgaagcg acagcgtatg ccgccgtgtt taagtttgac tggttctctg
     4561 ccaccggtac cgccattctg tttgctgccc tgctctcgat tgtctggctg aagatgaaac
     4621 catctgacgc tatcagcacc ttcagcagca cgctgaaaga actggctctg cctatctact
     4681 ccatcggcat ggtgctggcg ttcgccttta tctcgaacta ttccggactg tcatcaacac
     4741 tggcgctggc actggcgcac accggtcatg cattcacctt cttctcgccg ttcctcggct
     4801 ggcttggggt attcctgacc gggtcggata cgtcatctaa tgctttgttt gccgctttgc
     4861 aggctaccgc cgcacaacaa attggcgttt ctgacctgtt gctggttgca gccaacacca
     4921 ccggtggtgt caccggtaag atgatctccc cgcaatctat cgctatcgcc tgtgcggcgg
     4981 taggcctggt gggcaaagag tctgatttgt tccgctttac tgtcaaacac agcctgatct
     5041 tcacctgtat ggtgggcgtg atcaccacgc ttcaggccta tgtcttaacg tggatgattc
     5101 cttaatgatt gttttaccca gacgcctgtc agacgaggtt gccgatcgtg tgcgggcgct
     5161 gattgatgaa aaaaacctgg aagcgggcat gaagttgccc gctgaacgtc aactggcgat
     5221 gcaactcggc gtgtcgagaa attcactgcg cgaggcgctg gcgaaactgg taagcgaagg
     5281 tgtgctgctc agtcgacgtg gcggcgggac gtttattcgc tggcgtcatg acacatggtc
     5341 ggagcaaaac atcgtccagc cgctgaaaac gctgatggcc gatgatccgg attacagttt
     5401 cgatattctg gaagcccgct acgccattga agccagcacc gcatggcatg cagcaatgcg
     5461 cgccacacct ggcgaaaaag aaaagattca gctttgcttt gaagcaacgc tcagcgaaga
     5521 cccggatctc gcctcacaag cggacgttcg ttttcatctg gcgattgccg aagcctcaca
     5581 taacatcgtg ctgctgcaaa ccatgcgtgg tttcttcgat gtcctgcaat cctctgtgaa
     5641 gcatagccgc cagcggatgt acctggtacc cccggttttt tcacagctga ccgaacaaca
     5701 tcaggcggtc attgacgcca tttttgccgg tgatgctgac ggggcgcgta aagcaatgat
     5761 ggcgcacctt agctttgttc acaccaccat gaaacgattc gatgaagatc aggctcgcca
     5821 tgcacgcatt acccgcctgc ctggtgacca taatgagcat tcgagggaga aaaacgcatg
     5881 attatttccg cagccagcga ttatcgcgcc gcagcgcaac gcattctgcc gccgttcctg
     5941 ttccactata tggatggggg ggcatattct gaatacacgc tgcgccgcaa cgtggaagat
     6001 ttgtcagaag tggcgctgcg ccagcgtatt ctgaaaaaca tgtctgactt aagcctggaa
     6061 acgacgctgt ttaatgagaa attgtcgatg ccggtggcgc taggtccggt aggtttgtgt
     6121 ggcatgtatg cgcgacgcgg cgaagttcag gctgccaaag cagcggatgc gcatggcatt
     6181 ccgtttactc tctcgacggt ttccgtttgc ccgattgaag aagtggctcc ggctatcaaa
     6241 cgtccgatgt ggttccagct ttatgtgctg cgcgatcgcg gctttatgcg taacgcactg
     6301 gagcgagcaa aagcagcggg ttgttcgacg ctggtattca ccgtggatat gccaacaccg
     6361 ggagcgcgtt atcgtgatgc gcattctggg atgagcggcc cgaacgcggc aatgcgccgc
     6421 tatttgcagg cggtgacgca tccgcaatgg gcgtgggatg tgggcctgaa cggtcgtccg
     6481 catgatttag gtaatatctc agcttatctc ggcaaaccga ccggactgga agattacatc
     6541 ggctggctgg ggaataactt cgatccgtcc atctcatgga aagaccttga gtggatccgc
     6601 gatttctggg atggcccgat ggtgatcaaa gggatcctcg atccggaaga tgcgcgcgat
     6661 gcagtacgtt ttggtgctga tgggattgtg gtttctaacc acggtggccg ccagctggac
     6721 ggtgtactct cttccgctcg tgcgttgccc gctattgcag atgcggtgaa aggtgatatc
     6781 gccattctgg cggatagcgg aatacgtaac gggcttgatg tcgtgcgtat gattgcgctc
     6841 ggtgccgaca ccgtactgct gggtcgtgct ttcctgtatg cgctggcaac agcgggccag
     6901 gcgggtgtag ctaacctgct aaatctgatc gaaaaagaga tgaaagtgtc gatgacgctg
     6961 actggcgcga aatcgattag cgaaattacg caagattcgc tggtgcaaga gctgagtaaa
     7021 gcgccggcgg cggcgctggc tccaatggcg aaagggaatg cagcttaagg gttagatgaa
     7081 tatctgctat cctgcccccg cactaagggg gcagtatgct aaacatcgta ctttacgaac
     7141 cagaaattcc gccaaatact ggcaacatca tccgtctttg cgctaatacc ggctttcgtc
     7201 tgcatatcat cgaaccaatg ggatttgcct gggacgataa gcgcctgcgc cgcgcggggc
     7261 tggactatca cgagtttacc gccgttacgc gtcatcatga ctatcgcgcg tttctcgaag
     7321 cagaaaatcc ccagcgcctg tttgccctca ccacgaaagg tacgcctgct cacagcgccg
     7381 taagctatca ggatggcgat tatctgatgt tcggcccgga aacacgcggc ctgccagcga
     7441 gcattcttga tgccctgccc gctgaacaaa aaattcgtat tccaatggtg ccggacagtc
     7501 gcagcatgaa cctgtccaat gcggtgtcgg tcgtggtgta tgaagcctgg cggcagttgg
     7561 ggtatccggg agcggtgttg agagattagt tactgtaggc cggatagatg attacatcgc
     7621 atccggcacg atcacaggac atcagatccc atcaccatac tcaaacgtat ggttaatacc
     7681 gttgaagtgc tggtccatat ccattgatgg cttatcgctg tctggtttac cgacaatgcg
     7741 tgccggaacg ccagcggcag tggtgtgcgg cggcaccggt tgcagcacca cggaacctgc
     7801 gccaatcttc gcgccgcgcc caacttcaat attaccgaga attttcgcgc ccgcgccaat
     7861 catcacgcct tcacgaattt tcgggtgacg gtcaccacca gatttacccg taccgccaag
     7921 cgtcacagat tgcagaatcg atacgtcgtt ttcaatcacc gccgtttcac ccacaacgat
     7981 gcctgtcgcg tggtcaagca tgataccgcg accaattttt gctgccgggt gaatatcaac
     8041 ctggaacgtt acagagacct ggttttgtag aaaaatagcc agcgcgcgac ggccctgatt
     8101 ccacaaccag tgaccgatgc gatacgcctg caaagcatga aaacccttca gatatagcaa
     8161 cggggttgag tatttatcca ccgccgggtc gcgggtacgc actgcctgaa tatcacaggc
     8221 cgcagaagcg atcatttccg ggtcggcggc gtaggcttct tccaccactt cacggatagc
     8281 aatagccggc ataatgggcg atgacagctt gttcgccagc atgtagctca gtgcactgcc
     8341 aaggttttcg tgcttgagta gcgtcgcgtg gtaaaaactg gccagcattg gctcacagtc
     8401 cgccagcgtt ctggcttcag ctttaatatt gttccagaca atttccagtt cttcacacga
     8461 cattgcttac tccacacgat aagataatga ccgacccgtt ctgcgcgggc cgggtcatag
     8521 tggtaacaaa ggttccctgg gtttagtggc tgctgcgctc gtccttgcgt gcacgaccta
     8581 gtaaagtcaa tgctgcctcg cgcgcgtttt ttccgcaata taatacttga taaatttcct
     8641 cggttattgg catttcaacg ccgaagcgat gcgccagttc gcggacttct ttcgtattgc
     8701 ggtagccttc caccacctga cctatctttt cctgcgcgct ttgtacatcc atgccctgac
     8761 cgagcatcat gccaaaacgg cggttacgcg actggttgtc ggtacaggta agcaccagat
     8821 cgcccagccc cgccattccc ataaaggtgg caggatcggc acccagcgcc gcgccaagac
     8881 gcgacatctc agccagccca cgagtgatca gcgccgtacg cgcattcgcg ccaaaaccga
     8941 taccgtcgga catccccgcg ccaatggcaa tgacgttttt caccgcgccg ccaagctgca
     9001 caccgatgaa atccgggttg ctgtaaacgc ggaaactttt gccacagtgc agcaattgct
     9061 ggaggtcatc ggcaaaggtc tggtccgtcg aggccagcga aatggccgtc ggtaaacctg
     9121 ccgccagttc tttcgcaaac gttgggccag agataaccgc cagcggaatt tgatcgccta
     9181 aggcctcacg cgccacgtcc tgtaacagac gtccggtttc tgcttccagc cctttggtcg
     9241 cccacaccag acgcgcatca ggacgcatca gcggtttaat ctggcgcagc acttcaccaa
     9301 agacatggct gggtacgacg acgagaatat tacggctggc tgccagcgca gtggcgagat
     9361 cgctttcaag atggagcgta tcgggaaaag gcacatcggg gagaaacgcg gcgttacagc
     9421 ggtcgcgttc aagcgttgcg atatgttcag ggtcatggcc ccagaggaca acctcgtggc
     9481 catttcttgc cagggtaatg gcaagagcgg tgccgtacga gccggcaccg atcacagtca
     9541 ttgaagcatt acgttggttc atcaggcatc ctgatgttct tcagtacctt cgccagcctg
     9601 ctgctgcaaa tagttcatga acagcgcatc gaagttaacc ggcgcaaggt tcagttgcgg
     9661 gaatgtaccg cgggatacca tgctggtgat gcactcacga gcatacggga acagaatgtt
     9721 cgggcagtat gcgcccaggc aatgtgccat ctgggtgcct tcgatacccg cgatggagaa
     9781 aataccgccc tgctgaactt cacacaggaa cgcggtttct tcgcccaggg aggccgttac
     9841 ggtaacacgc agtaccactt cgtaaacgtc atctgccagt tgagtagatg ccgtatccag
     9901 atcaagttta acttctggtt gccaatcttt ctggaaaacg tgcggcgcgt tcggcgcttc
     9961 gaaagaaata tccttggtat aaatacgttg gatctggaaa gtcatttcgg tgttgttttg
    10021 ttctgacatg tgtagaaaac ccttaagtgt tgtccttaaa atactgcgca atgtaatgcc
    10081 aacgttcaca cgttatttca gcaggggatc cagtccacca cgtgcatcca atgcatacaa
    10141 gtcatcacag ccaccaatgt gctgtgcgtc aataaaaatc tgtggaaccg tggtgcgacc
    10201 gctgcgtttg atcatctctt cacgcttagc ggcattgcca tcgatcggca actcctgaaa
    10261 actcacgccc ttgctgctca gcagtgcttt tgcacgatgg caatacgggc aggtttcttt
    10321 ggtatagatt tcaacattgg ccataaatta gctcctgttt tttaccctga aatttcatgt
    10381 tgcagggcgg cagcaaaatg gagtgtcccc agaagcttat aacaataagt cgctgggggc
    10441 ctaattgcag ctaacggcct gcatcatgaa aaacgacagg taaattattt gccgcgcacc
    10501 aaaggcaagt tttcgccagc ccagccagcg acgccttctt tcagtacgaa tacttgcgca
    10561 aaaccagctt tcgtcagcgc gtttgcaggc tcctggcact gcatgccaga accatctacc
    10621 acgataaccg gtttgtcttt gtgcttctca agctcaccaa cattgttggc tttgatttcg
    10681 ctcggcaaca ggttgataga acctgcgata tggcctttac ggaagtcatc acgctgacgt
    10741 aaatccacaa ccacagcgtc ttctttgttg atcagacgcg tagcttcacc acgagtaatc
    10801 accttcactt tcgaggtcag gctcttaaac gtagtcacaa gaaccgccac cagtaacgcg
    10861 atccaggcga tactcagtat gggatgacgg ccaacaaatt gcataatttc ttgcatgggg
    10921 ggtaacaact cccgacgtag tgattaaaaa aaccaggaaa ggagtatacc tgcgcagtgc
    10981 ggcaaataca gccagcgcgt taactggaat gcaattttgc ggggcgctac gaaaaaaaag
    11041 cgtaaatctg gcatctgctt gccgcgagcg gtcgtatatt ttgatcttca gaggctattt
    11101 tatcgattca gctgtagtaa aattacgcaa attttgactc ttgagtatga ggttgtcgca
    11161 atgtcggttt ctaaaaaacc tatggtactg gtgattctgg atggctatgg ctatcgcgaa
    11221 gaacagcagg ataacgccat ttttagtgct aaaaccccgg taatggatgc actgtgggcc
    11281 aatcgtccgc ataccctgat cgacgcttcc ggtttggaag tcggtctgcc tgaccgtcag
    11341 atgggcaact ccgaagtagg ccacgttaac ctgggtgccg gccgcatcgt gtatcaggac
    11401 ctgactcgtc tggacgttga aatcaaagat cgcgctttct ttgctaatcc ggtgttgact
    11461 ggtgcagtag ataaagcgaa gaacgcaggc aaagcagtac acattatggg tctgctctcc
    11521 gcaggtggtg tacacagcca cgaagatcac atcatggcga tggtagaact ggcagctgaa
    11581 cgcggcgcag aaaaaatcta cctacacgca ttccttgacg gtcgcgacac tccgccgcgc
    11641 agtgctgaat cctcgctgaa aaaattcgaa gagaaatttg ccgcgctggg taaaggccgc
    11701 gtagcgtcca tcattggtcg ttactacgcg atggaccgcg ataaccgttg ggatcgcgtt
    11761 gaaaaagctt atgacctgct gactctggcg caaggtgagt tccaggccga taccgccgtt
    11821 gccggtttgc aggctgctta tgctcgcgac gaaaacgacg agttcgtgaa agcgaccgtt
    11881 attcgtgcag aaggtcagcc agatgcggca atggaagacg gcgatgcact gattttcatg
    11941 aacttccgtg ctgaccgcgc gcgtgaaatc actcgtgcat tcgtaaatgc cgattttgac
    12001 ggcttcgcgc gtaagaaagt ggttaacgtc gatttcgtga tgctgaccga atacgctgcc
    12061 gacatcaaaa ccgcagtggc ttacccaccc gcttccctgg ttaacacctt cggcgaatgg
    12121 atggcgaaaa acgacaaaac tcagctgcgt atttccgaaa ccgaaaaata tgcccacgtt
    12181 actttcttct tcaacggtgg cgtagaagag tcgttcaaag gcgaagatcg cattctgatc
    12241 aactcaccga aagtggctac ctacgatctg caaccggaaa tgagctccgc agagctgacc
    12301 gaaaaactgg ttgcggcaat caagagcggc aaatacgaca ccattatctg taactatccg
    12361 aacggcgaca tggtaggtca caccggagtg atggaagcgg cggttaaagc ggttgaagcg
    12421 ctggatcact gcgtggaaga agtcgcgaaa gcggttgagt ccgtgggtgg acaactgctg
    12481 atcaccgctg accacggtaa cgctgaacag atgcgcgatc cggcaacggg tcaggcacac
    12541 acagcacaca ccaacctgcc agttccgctg atttacgttg gtgataagaa cgtgaaagcg
    12601 gttgaaggcg gcaaactttc tgacatcgcg ccgaccatgt tgtcgctgat gggtatggaa
    12661 atcccgcaag agatgactgg taagccgctg ttcatcgtgg aataatccct ccccatgagg
    12721 ggaaaggcga ttaataccat gacacgggcc gtgaaaccgc gcaggtttgc aatcaggccc
    12781 atcatctacg ccagcgttct gagcgctggc gtattgttgt gcgccttttc cgcccacgcg
    12841 gatgagcgtg accaactcaa atccattcag gctgacatcg ccgcgaaaga gcgcgcggta
    12901 cgccaaaagc aacaacaacg cgcaagcctg ctcgcacaat tgaaaaagca ggaagaagcg
    12961 atctctgaag ccacccgtaa actgcgcgaa acgcaaaaca cgcttaatca actgaataaa
    13021 cagattgatg agatgaacgc gtcgattgcc aaactggagc agcaaaaagc cgcccaggag
    13081 cgcagcctcg ccgcgcaact ggatgccgcg tttcgtcagg gtgaacatac cggtattcag
    13141 ctgattctca gcggtgaaga aagccagcgt gggcagcggt tacaggctta tttcggctat
    13201 ctcaaccagg cgcgacaaga aaccattgct cagttgaaac aaactcgtga agaagtcgct
    13261 atgcagcgtg ccgaactgga agagaaacaa agcgagcaac aaacgctttt atatgagcag
    13321 cgcgcccaac aggcgaagct gacccaggcg ttgagcgagc gtaagaaaac actggcaggg
    13381 ctggagtctt ccatccagca aggtcagcaa cagttgagcg agctgcgcgc caacgaatcc
    13441 cgcctgcgta acagcattgc ccgtgcggaa gctgcggcga aagcgcgtgc tgaacgtgaa
    13501 gcgcgcgaag cccaggcggt tcgcgaccgc cagaaagaag cgacgcgcaa aggcaccacc
    13561 tacaagccga ccgaaagcga aaaatcgctg atgtcccgta ccggtggtct gggcgcaccg
    13621 cgcggtcagg cattctggcc agttcgcggg ccgacgctgc atcgctatgg cgaacagcta
    13681 cagggtgaat tacgctggaa agggatggtg attggtgcct ctgaaggtac tgaagttaaa
    13741 gcgattgccg acggccgggt gattctggct gactggctgc aaggctacgg tctggtggtg
    13801 gtggttgagc atggtaaagg cgacatgagt ctttacggct ataatcagag cgcactggtg
    13861 agcgttggtt cgcaggttcg cgcgggccag ccaattgcac tggtgggcag cagtggcggt
    13921 cagggtcggc cttcactcta tttcgaaatt cgccgccagg gtcaggcggt caatccacag
    13981 ccgtggttgg gaagataagt tttgtttcca tttcgtcgta acgttcttgc atttgccgct
    14041 ctgttggcgc tctcctcccc cgtaattgct ggcaaacttg ccatcgtcat tgatgatttt
    14101 gggtatcgcc cgcacaacga aaaccaggtg ctggcgatgc cttccgctat ctccgtcgct
    14161 gtattacccg attcaccgca cgccagagag atggcgacca aagcacataa cagcggtcac
    14221 gaagtgttga ttcatctacc gatggcacca ctgagtaaac agccgctgga gaaaaatacg
    14281 ctacgcccgg agatgagcag cgacgaaatt gagcgcatta ttcgtagtgc ggtcaataac
    14341 gtgccctatg ccgtggggat caacaaccac atgggcagca agatgacctc taacctgttt
    14401 ggtatgcaga aagtgatgca ggcgctggag cgttacaatc tttacttcct cgacagcgta
    14461 accatcggta atacccaggc gatgcgcgcc gcgcaaggca ctggcgtgaa ggtgatcaaa
    14521 cggaaggtgt tcctcgacga ttcgcagaac gaagcggaca tccgtgtgca atttaatcgc
    14581 gcaattgacc tggcgcgtcg caacggttca accattgcca ttgggcatcc tcacccttca
    14641 acggtacgcg tgctacaaca gatggtttat aacctgccgc cagacattac gctggtgaaa
    14701 gccagcagct tgttgaatga accgcaggtt gatacgtcta caccgccgaa aaacactgtg
    14761 cccgacacac cgcgtaatcc attccatggc gtgaagctgt gcaaaccgaa gaagccgata
    14821 gaacctgttt atgctaatcg cttctttgaa gtattaagcg aaagcatcag ccagagcacg
    14881 ctgatcgttt acttccagca tcagtggcaa ggctggggca aacagcccga atcggcaaaa
    14941 cttaacgcta gcgcaaatta aaggcgctac gcgtaatgcg atggtgcgac cgcgttttgt
    15001 cgcgccattg ccataagcgg aaagaccaca gtaacgcctg gtaacccacc ttcacgctgc
    15061 ggacattggt aatcaggcgc ttatacatac cggaagtaaa tatctcggca atcatccgtt
    15121 ggcgggtaag aatatccggc tctttgcgca ccgcatggca aacacgcaat gcttcgtaag
    15181 ttatttgctg atgaaattcc ggataaatcg taattttgtc agcataattt cgatttaatt
    15241 tctccagcag gcgggtaatc ttaatatagt gacgttgata attaaggttt ttgttccctt
    15301 gtctgttcaa ccgactcact gacgtattat gcagataata tttatataat gattgctcgg
    15361 tatatcgcgc acgcagcgcg ttaaacataa attctgttgt ccagacaata tcctgatgat
    15421 gtaatccggc aataaattta atgttatttt taacaataac atcgcggcga taaaccccca
    15481 tccagaccac gtgtgtccaa cggcgggaag aaagtcccat ccgcagccag tccggaccag
    15541 ttaatacacc ggttgagcga aggcgatcgg tagggatgga ttgccaggtt tcacccgttt
    15601 cacgaaaaca ccaatcagcg ttgcactgcg ccacgtcgag gtcgtcctct aacgccatgg
    15661 tcatcagcgt ttcgtacatg gtgggataga cctcatcgtc agcatcgaca aaagcgacat
    15721 attttcccgt tgccacttca atcccacgat tacgcgccac cgatgcgcca gcattcgcct
    15781 gatgcaacaa acgaacgtgc ggatagtttt ctgcgtaatg ctttgctatt tcaacagaat
    15841 tatccgttga accatcgtta ataataatga tttccagagc agtccatgtt tgcgtaatta
    15901 aagattccat acaagtgcgg aaatcatcgc ccgcattata taacggaata ataacactaa
    15961 gtttattggt gctgttcatc ataaattacc gacgacctct gaagttatcc tgacgtttta
    16021 caggctttgt attaagaaac tattaagcct gtgagatatt atttttaccg gtgaaagagt
    16081 cacttcaggg tagagagata atgagagcag ctatataaga gatcagtgca attacgaact
    16141 ctttattaca ctgacaggat tttacatatt taacggatta tcctcaggca aaagggctgg
    16201 aataccagcc cttgttcgtg ttaatcccag ctcagaataa ctttcccgga ctggcccgaa
    16261 cgcatggcat caaagccctt ctggaaatca tcgatagaga aacgatgggt aatgatcggc
    16321 gagagatcga ggccagactg aatcagcgcc gccatcttgt accaggtttc aaacatctca
    16381 cgaccgtaaa tacctttaat gaacaagcct ttaaagatta ctttggtcca gtcgatagac
    16441 atatcggacg gtggaatacc cagcatcgca atacgaccgc cgtggttcat ggtgtcgagc
    16501 atggtacgaa acgctggcgg cgcaccggac atttccagac cgacatcaaa gccttcggtc
    16561 atgcccagtt ctgtcatcac atcattaagg ttttctttag cgacgttaac cgcacgggtg
    16621 atacccattt tgcgtgccag ctcaaggcgg tattcgttaa catcagtgat caccacattg
    16681 cgtgcaccaa cgtgtttcgc caccgccgca gccataatac ctatcggacc tgcaccagag
    16741 accagcacat cctcgccaac cagatcgaac gacagcgcgg tatgcacagc gttaccgaag
    16801 ggatcaaaaa tggaagccag gtcgtcggag atattgtccg ggattttgaa ggcgttgaac
    16861 gccggaatca ccagatactc ggcgaagcag cccggacggt taacaccgac gccgatcgtg
    16921 ttgcggcaca gatgggtacg accgccacga cagttgcgac agtgaccgca ggtgatatgg
    16981 ccttcgccag aaacgcgatc gccaatcttg aagcctttca cttcctgacc aatacctacc
    17041 acttcaccga catattcatg gcccacgacc atcggcaccg ggatggtttt ttgcgaccac
    17101 tcatcccagt tatagatgtg aacgtcagtc ccgcagatgg ctgttttacg gattttaatc
    17161 agcagatcgt tatgcccgag ttccggtaca ggaacgtcgg tcatccagat gccctcttcc
    17221 gctttcagtt tggataacgc tttcatctca caccctcagg cgataacgcc cagttgttta
    17281 ccaatacgcg tgaatgcttc tactgcacgc gtaatttgct caggggtatg cgccgcagac
    17341 atctgggtac gaatacgcgc ctgacctttc ggaacgaccg gatagaagaa accggtgacg
    17401 taaatgccct ctttttgcag ctcacgggca aatttctgcg ccactaccgc atcgccaagc
    17461 atgacgggaa taatggcgtg atcggctccc gccagggtaa agcccgccgc cgacatttgc
    17521 tcacggaact gacgcgcgtt cgcccacaga cggtcacgca gctcgctacc cgcttcgacc
    17581 atctccagca ctttgatgga tgcggcgaca atcgctggtg ccagcgagtt ggagaacagg
    17641 tacggacgag aacgctggcg cagccactca accacttctt tgcgcgccgc ggtataacca
    17701 ccagaagccc cgcccaacgc tttacccaac gtaccggtaa tgatgtctac acgtcccatc
    17761 acatcgcagt attcatggga accacgacca ttttcaccga caaaaccgac cgcgtgggag
    17821 tcgtctacca tcaccagggc atcgtattta tctgccagat cgcaaacgcc cttcaggttg
    17881 gcaatcacgc cgtccatcga gaacacacca tcggtggcaa tcagcacatg acgcgcaccg
    17941 gcttcacgcg cttctttcag acgagcttcc agctcatgca tatcgttgtt ggcataacga
    18001 tagcgtttag ctttgcacag acgcacacca tcaataatag acgcgtggtt cagtgcgtcg
    18061 gagataatgg cgtcctccgc gctcagcagc gtttcaaaca ggccgccgtt agcatcaaag
    18121 caggaagagt agagaatcgc atcttccatc cccaggaatg ccgccagttt ttgttcaagc
    18181 tctttatggc tgtcctgagt gccgcaaata aaacgcaccg aagccatgcc gaaaccgtga
    18241 gaatccattc ccgcctttgc cgccgcaatc agatccggat gattcgccag tccgagatag
    18301 ttgttggcac aaaagttaat gacgtggctt ccatcagcca cagtgatatc tgcttgctgc
    18361 gcagacgtaa taatgcgctc ttctttaaac aacccttccg cccgtgcggt ttccagatcg
    18421 ttggttaact gctgataaaa ttctccacgc attgcgattc tccagacagg gcaaattcca
    18481 gcacatatta cccaaacttc taggtcgcga cgagatgacg cgttaacact tctgcaaaat
    18541 tcaggataaa taacgtgtat cttgatgtct cttaacacct acagatagta gaaaaactgt
    18601 acagttttgg ctgggcataa tgatgagtga ttttactgca ttgatataaa aagaaaaaat
    18661 gatgccagag catcattttt ccaatctcat ttggtatcaa aacgctaccc actaatggag
    18721 ggaaacctat tttcttgaaa ggaaaattct cagcgcctgc atttttgccc ctaaagtgtt
    18781 actcctgaag cagcgaaaca aatcgaacca ttttcctctc cccagatagt ttaggttgaa
    18841 gagatcggaa cgtttgtaat agcacatgac aaaaatgccc tggtcatcgt ccacaatgtt
    18901 attgtttagg gttatttttt gactttccag aactaattta tagaattctt tccatttatg
    18961 ctgtgaacca acaattgcgc caccaataat atatacatga ttgccaatca tgaaatcaaa
    19021 agcctgttgt tgagatgtaa cagcaagccc tttttttata gtgaaaagat gcatcttatt
    19081 ttcatcaaat gggaaatccc aaattttcaa tcctcgagtt acatttggct tacggcaata
    19141 gccaaaatct atccacgcaa caagaggagt tttcactaag cccatgttga tagctttatt
    19201 tacaaaatag gccttaaggt tacataccaa tacatactct ggtgaccagt actctgggtt
    19261 ttttaattgc cgtggctcaa gtctatttgt aaacgactca tctttttgaa ttttttcgat
    19321 acgacttctg atatatctaa atttcttttt tatatcgata acgataaccg ttgttggttt
    19381 tccgttgcga atcgcttcaa cccttgattt caaatcggga gaagtgaaaa taatcatttc
    19441 attttcaagt gcggccagac gctcaaagta actaaaataa acatcaactg aacgagctag
    19501 tttttcacgg aaccctttat tagcagtcca atcccctcta ccaatgtcaa aatatgcagt
    19561 gataattgtg gtggatgatt tcattactaa taagaccatt aatataaaaa aag
//
LOCUS       DADSYM010000091        19475 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2415
            SAMN08039579-rid11723173.denovo.091, whole genome shotgun sequence.
ACCESSION   DADSYM010000091 DADSYM010000000
VERSION     DADSYM010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039579
            Sequence Read Archive: SRR6362533
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 19475)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19475)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980162.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 03:39:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,484
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 4,347
            CDSs (with protein)               :: 4,347
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 43 of 85
            Pseudo Genes (incomplete)         :: 37 of 85
            Pseudo Genes (internal stop)      :: 19 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19475
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039579-rid11723173.denovo.091"
                     /strain="LGE2415"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8302 N 95.9628 W"
                     /collection_date="2015-08-24"
     gene            183..518
                     /locus_tag="J1Y83_002772"
     CDS             183..518
                     /locus_tag="J1Y83_002772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004856597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phnA family protein"
                     /protein_id="HBA7630906.1"
                     /translation="MSLPHCPKCNSEYTYEDNGMYICPECAYEWNDAEPAQESDELIV
                     KDANGNLLADGDSVTIIKDLKVKGSSSMLKIGTKVKNIRLVEGDHNIDCKIDGFGPMK
                     LKSEFVKKN"
     gene            678..1121
                     /gene="yjdN"
                     /locus_tag="J1Y83_002773"
     CDS             678..1121
                     /gene="yjdN"
                     /locus_tag="J1Y83_002773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family metalloprotein YjdN"
                     /protein_id="HBA7630907.1"
                     /translation="MPLSPYLSFAGNCSDAIAYYQRTLGAELLYKISFGEMPKSAQDS
                     AENCPSGMQFPDTAIAHANVRIAGSDIMMSDAMPSGKASYSGFTLVLDSQQVEEGKRW
                     FDNLAANGKIEMAWQETFWAHGFGKVTDKFGVPWMINVIKQQPTQ"
     gene            1254..2042
                     /gene="phnC"
                     /locus_tag="J1Y83_002774"
     CDS             1254..2042
                     /gene="phnC"
                     /locus_tag="J1Y83_002774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter ATP-binding protein"
                     /protein_id="HBA7630908.1"
                     /translation="MQTIIRVEKLAKTFNQHQALHAVDLNIHHGEMVALLGPSGSGKS
                     TLLRHLSGLITGDKSVGSHIELLGRTVQREGRLARDIRKSRAHTGYIFQQFNLVNRLS
                     VLENVLIGALGSTPFWRTCFSYFTREQKQRALQALTRVGMVHFAHQRVSTLSGGQQQR
                     VAIARALMQQAKVILADEPIASLDPESARIVMDTLRDINQNDGITVVVTLHQVDYALR
                     YCERIVALRQGHVFYDGCSQQFDNERFDHLYRSINRVEENAKAA"
     gene            2067..3083
                     /gene="phnD"
                     /locus_tag="J1Y83_002775"
     CDS             2067..3083
                     /gene="phnD"
                     /locus_tag="J1Y83_002775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter substrate-binding
                     protein"
                     /protein_id="HBA7630909.1"
                     /translation="MNAKIIASLAFTSMFSLSTLLNPAYAEEQEKALNFGIISTESQQ
                     NLKPQWTPFLQDMEKKLGVKVNAFFAPDYAGIIQGMRFNKVDIAWYGNLSAMEAVDRA
                     NGQVFAQTVAADGSPGYWSVLIVNKDSPINNLNDLLAKRKDLTFGNGDPNSTSGFLVP
                     GYYVFAKNNISASDFKRTVNAGHETNALAVANKQVDVATNNTENLDKLKTSAPEKLKE
                     LKVIWKSPLIPGDPIVWRKNLSETTKDKIYDFFMNYGKTPEEKAVLERLGWAPFRASS
                     DLQLVPIRQLALFKEMQSVKDNKGLNEQDKLAKTTAIQAQLDDLDRLNNALSAMSSVS
                     KAVQ"
     gene            3189..3968
                     /gene="phnE"
                     /locus_tag="J1Y83_002776"
     CDS             3189..3968
                     /gene="phnE"
                     /locus_tag="J1Y83_002776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313113.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter, permease protein
                     PhnE"
                     /protein_id="HBA7630910.1"
                     /translation="MQTITIAPPKRSWFSLLSWAVVIAVLIASWQGAEMAPLTLIKDG
                     GNMATFAADFFPPDFSQWQDYLTEMAVTLQIAVWGTALAVVLSIPFGLMSAENLVPWW
                     VYQPVRRLMDACRAINEMVFAMLFVVAVGLGPFAGVLALFIHTTGVLSKLLSEAVEAI
                     EPGPVEGIRATGANKLEEILYGVLPQVMPLLISYSLYRFESNVRSATVVGMVGAGGIG
                     VTLWEAIRGFQFQQTCALMVLIIVTVSLLDFLSQRLRKHFI"
     gene            3989..4714
                     /gene="phnF"
                     /locus_tag="J1Y83_002777"
     CDS             3989..4714
                     /gene="phnF"
                     /locus_tag="J1Y83_002777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate metabolism transcriptional regulator
                     PhnF"
                     /protein_id="HBA7630911.1"
                     /translation="MHLSTHPTSYPTRYQEIAAKLEQELRQHYRCGDYLPAEQQLAAR
                     FEVNRHTLRRAIDQLVEKGWVQRRQGVGVLVLMRPFDYPLNAQARFSQNLLDQGSHPT
                     SEKLLSVLRPASGHVADALGITEGENVIHLRTLRRVNGVALCLIDHYFADLTLWPTLQ
                     CFDSGSLHDFLREQTGITLRRSQTRISARRAQAKECQRLEIPNMSPLLCVRTLNHRDG
                     ESSPAEYSVSLTRADMIEFTMEH"
     gene            4715..5167
                     /gene="phnG"
                     /locus_tag="J1Y83_002778"
     CDS             4715..5167
                     /gene="phnG"
                     /locus_tag="J1Y83_002778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnG"
                     /protein_id="HBA7630912.1"
                     /translation="MHADTATRQHWMSVLAHSQPAELAARLNALNINADYEVIRAAET
                     GLVQIQARMGGTGERFFAGDATLTRAAVRLTDGTLGYSWVLGRDKQHAERCALIDALM
                     QQNRHFQNLSETLITPLDADRMARIAARQAEVNASRVDFFTMVRGDNA"
     gene            5164..5748
                     /gene="phnH"
                     /locus_tag="J1Y83_002779"
     CDS             5164..5748
                     /gene="phnH"
                     /locus_tag="J1Y83_002779"
                     /EC_number="2.7.8.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnH"
                     /protein_id="HBA7630913.1"
                     /translation="MTLETAFMLPVQDAQHSFRRLLKAMSEPGVIVALHQLKRGWQPL
                     NIATTSVLLTLADNDTPVWLAAPLSNDIVNQSLRFHTNASLVNQPEQATFAVTDEAIS
                     SEQLNALSTGTAVAPEAGATLILQVTSLSGGRMLRLTGAGIAEERMIAPQLPKCILHE
                     LTERPHPFPLGIDLILTCGERLLAIPRTTHVEVC"
     gene            5748..6812
                     /gene="phnI"
                     /locus_tag="J1Y83_002780"
     CDS             5748..6812
                     /gene="phnI"
                     /locus_tag="J1Y83_002780"
                     /EC_number="2.7.8.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate
                     5-triphosphate synthase subunit PhnI"
                     /protein_id="HBA7630914.1"
                     /translation="MYVAVKGGEKAIDAAHALQESRRRGDTDLPELSVAQIEQQLNLA
                     VDRVMTEGGIADRELAALALKQASGDNVEAIFLLRAYRTTLAKLAVSEPLDTTGMRLE
                     RRISAIYKDIPGGQLLGPTYDYTHRLLDFTLLANGETPTLTTADSEQQPSPHVFSLLA
                     RQGLAKFEEDSGAQPDDITRTPPVYPCSRSSRLQQLMRGDEGYLLALAYSTQRGYGRN
                     HPFAGEIRSGYIDIFIVPEELGFAVNVGELLMTECEMVNGFIDPPDEPPHFTRGYGLV
                     FGMSERKAMAMALVDRALQAPEYGEHATGPAQDEEFVLAHADNVEAAGFVSHLKLPHY
                     VDFQAELELLKRLQQEQNHG"
     gene            6805..7650
                     /gene="phnJ"
                     /locus_tag="J1Y83_002781"
     CDS             6805..7650
                     /gene="phnJ"
                     /locus_tag="J1Y83_002781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate 5-phosphate
                     C-P-lyase PhnJ"
                     /protein_id="HBA7630915.1"
                     /translation="MANLSGYNFAYLDEQTKRMIRRAILKAVAIPGYQVPFGGREMPM
                     PYGWGTGGIQLTASVIGESDVLKVIDQGADDTTNAVSIRNFFKRVTGVNTTERTDDAT
                     LIQTRHRIPETPLTEDQIVIFQVPIPEPLRFIEPRETETRTMHALEEYGVMQVKLYED
                     IARFGHIATTYAYPVKVNGRYVMDPSPIPKFDNPKMDMMPALQLFGAGREKRIYAVPP
                     FTRVESLDFDDHPFTVQQWDEPCAICGSTHSYLDEVVLDDAGNRMFVCSDTDYCRQQS
                     EAKNQ"
     gene            7647..8405
                     /gene="phnK"
                     /locus_tag="J1Y83_002782"
     CDS             7647..8405
                     /gene="phnK"
                     /locus_tag="J1Y83_002782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnK"
                     /protein_id="HBA7630916.1"
                     /translation="MNQPLLSVNNLTHLYAPGKGFSDVSFDLWPGEVLGIVGESGSGK
                     TTLLKSISARLTPQQGEIRYENRSLYAMSEADRRRLLRTEWGVVHQHPLDGLRRQVSA
                     GGNIGERLMATGARHYGDIRATAQKWLEEVEIPANRIDDLPTTFSGGMQQRLQIARNL
                     VTHPKLVFMDEPTGGLDVSVQARLLDLLRGLVMELNLAVVIVTHDLGVARLLADRLLV
                     MKQGKVVESGLTDRVLDDPHHPYTQLLVSSVLQN"
     gene            8516..9196
                     /gene="phnL"
                     /locus_tag="J1Y83_002783"
     CDS             8516..9196
                     /gene="phnL"
                     /locus_tag="J1Y83_002783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnL"
                     /protein_id="HBA7630917.1"
                     /translation="MINVQNVSKTFILHQQNGVRLPVLQGTSLTVNAGECLVLHGHSG
                     SGKSTLLRSLYANYLPDEGQIQIKHGDEWVDLVTAPARKVVEIRKTTVGWVSQFLRVI
                     PRISALEVVMQPLLDTGVPREACAAKAARLLTHLNVPERLWHLAPSTFSGGEQQRVNI
                     ARGFIVDYPILLLDEPTASLDAKNSAAVVELIREAKARGAAIVGIFHDETVREQVADR
                     LHPMGVSS"
     gene            9193..10329
                     /gene="phnM"
                     /locus_tag="J1Y83_002784"
     CDS             9193..10329
                     /gene="phnM"
                     /locus_tag="J1Y83_002784"
                     /EC_number="3.6.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate
                     5-triphosphate diphosphatase"
                     /protein_id="HBA7630918.1"
                     /translation="MIINNVKLVLENEVVHGSLEMQDGEIRAFAESQSHLSEAMDGEG
                     GWLLPGLIELHTDNLDKFFTPRPKVDWPAHSAMSSHDALMVASGITTVLDAVAIGDVR
                     DGGDRLENLEKMINAIEETQKRGVNRAEHRLHLRCELPHHTTLPLFEKLVQRELVTLV
                     SLMDHSPGQRQFANREKYREYYQGKYSLTDAQMQQYEEEQLALAARWSQPNRETIAAM
                     CRARHIALASHDDATHAHVAESHQLGSVIAEFPTTFEAAEASRKHGMNVLMGAPNIVR
                     GGSHSGNVAASELAQLGLLDILSSDYYPASLLDAAFRVADDESNRFTLPQAVKLVTKN
                     PAQALNLQDRGVIGEGKRADLVLAHRKGNHIHIDHVWRQGKRVF"
     gene            10329..10886
                     /gene="phnN"
                     /locus_tag="J1Y83_002785"
     CDS             10329..10886
                     /gene="phnN"
                     /locus_tag="J1Y83_002785"
                     /EC_number="2.7.4.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 1,5-bisphosphokinase"
                     /protein_id="HBA7630919.1"
                     /translation="MTGKLIWLMGASGSGKDSLLTELRQREQTQLLVAHRYITRAASA
                     GSENHIALREQEFFTRAGQNLLALSWHANGLYYGVGVEIDLWLHAGFDVVVNGSRAHL
                     PQARARYQSALLPVCLQVSPEILRQRLENRGRENASEINARLARAARYTPQDCLTLNN
                     DGSLRQSVDKLLTLIHQKEKHHACL"
     gene            10873..11307
                     /gene="phnO"
                     /locus_tag="J1Y83_002786"
     CDS             10873..11307
                     /gene="phnO"
                     /locus_tag="J1Y83_002786"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoalkylphosphonate N-acetyltransferase"
                     /protein_id="HBA7630920.1"
                     /translation="MPACELRPATQYDTDAVYALICELKQAEFDHQAFRVGFNANLRD
                     PNMRYHLALLDGEVVGMIGLHLQFHLHHVNWIGEIQELVVIPQARGLNVGSKLLAWAE
                     EEARQAGAEMTELSTNVKRHDAHRFYLREGYEQSHFRFTKAL"
     gene            11309..12067
                     /gene="phnP"
                     /locus_tag="J1Y83_002787"
     CDS             11309..12067
                     /gene="phnP"
                     /locus_tag="J1Y83_002787"
                     /EC_number="3.1.4.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate metabolism protein PhnP"
                     /protein_id="HBA7630921.1"
                     /translation="MSLTLTLTGTGGAQGVPAWGCECAACARARRSPQYRRQPCSGVV
                     KFNDAITLIDAGLHDLADRWSPGSFQQFLLTHYHMDHVQGLFPLRWGVGDTIPVYGPP
                     DEQGCDDLFKHPGLLDFSHTVEPFVVFDLQGLQVTPLPLNHSKLTFGYLLETAHSRVA
                     WLSDTAGLPEKTLKFLRNNQPQVMVIDCSHPPREDAPRNHCDLNTVLALNQVIRSPRV
                     ILTHISHQFDAWLMENALPSGFEAGFDGMDIEVA"
     gene            complement(12095..12424)
                     /gene="yjdP"
                     /locus_tag="J1Y83_002788"
     CDS             complement(12095..12424)
                     /gene="yjdP"
                     /locus_tag="J1Y83_002788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YjdP"
                     /protein_id="HBA7630922.1"
                     /translation="MKRFPLFLLFTLLTLSTVPAQADIIDDTIGNIQQAINDAYNPDR
                     GRDYEDSRDDGWQRQVSDDRRKQYNDRRRQFEDRRRQLDDRQRQLDQERRQLEDEERR
                     MEDEYGR"
     gene            complement(12493..12942)
                     /gene="rpiB"
                     /locus_tag="J1Y83_002789"
     CDS             complement(12493..12942)
                     /gene="rpiB"
                     /locus_tag="J1Y83_002789"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional allose-6-phosphate
                     isomerase/ribose-5-phosphate isomerase RpiB"
                     /protein_id="HBA7630923.1"
                     /translation="MKKIAFGCDHVGFILKHEIVAHLVERGVEVIDKGTWSSERTDYP
                     HYASQVALAVAGGEADGGILICGTGVGISIAANKFAGIRAVVCSEPYSAQLSRQHNDT
                     NVLAFGSRVVGLELAKMIVDAWLGAQYEGGRHQQRVEAITAIEQQRN"
     gene            13301..14191
                     /gene="alsR"
                     /locus_tag="J1Y83_002790"
                     /gene_synonym="rpiR"
     CDS             13301..14191
                     /gene="alsR"
                     /locus_tag="J1Y83_002790"
                     /gene_synonym="rpiR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418513.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="als operon DNA-binding transcriptional repressor
                     AlsR"
                     /protein_id="HBA7630924.1"
                     /translation="MSQSEFDSALPNGIGLAPYLRMKQEGMTENESRIVEWLLKPGNL
                     SCAPAIKDVAEALAVSEAMIVKVSKLLGFSGFRNLRSALEDYFSQSEQVLPSELAFDE
                     APQDVVNKVFNITLRTIMEGQSIVNVDEIHRAARFFYQARQRDLYGAGGSNAICADVQ
                     HKFLRIGVRCQAYPDAHIMMMSASLLQEGDVVLVVTHSGRTSDVKAAVELAKKNGAKI
                     ICITHSYHSPIAKLADYIICSPAPETPLLGRNASARILQLTLLDAFFVSVAQLNIEQA
                     NINMQKTGAIVDFFSPGALK"
     gene            14250..15185
                     /gene="alsB"
                     /locus_tag="J1Y83_002791"
     CDS             14250..15185
                     /gene="alsB"
                     /locus_tag="J1Y83_002791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-allose transporter substrate-binding protein"
                     /protein_id="HBA7630925.1"
                     /translation="MNKYLKYFSGTLVGLMLSTSAFAAAEYAVVLKTLSNPFWVDMKK
                     GIEDEAKTLGVSVDIFASPSEGDFQSQLQLFEDLSNKNYKGIAFAPLSSVNLVMPVAR
                     AWKKGIYLVNLDEKIDMDNLKKAGGNVEGFVTTDNVAVGAKGASFIIDKLGAEGGEVA
                     IIEGKAGNASGEARRNGATKAFKKASQIKLVASQPADWDRIKALDVATNVLQRNPNIK
                     AIYCANDTMAMGVAQAVANAGKTGKVLVVGTDGIPEARKMVEAGQMTATVAQNPADIG
                     ATGLKLMVDAEKSGKVIPLDKAPEFKLVDSILVTQ"
     gene            15312..16844
                     /gene="alsA"
                     /locus_tag="J1Y83_002792"
     CDS             15312..16844
                     /gene="alsA"
                     /locus_tag="J1Y83_002792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-allose ABC transporter ATP-binding protein
                     AlsA"
                     /protein_id="HBA7630926.1"
                     /translation="MATPYISMAGIGKSFGPVHALKSVNLTVYPGEIHALLGENGAGK
                     STLMKVLSGIHEPTKGTITINNINYNKLDHKLAAQLGIGIIYQELSVIDELTVLENLY
                     IGRHLTKKICGVNIIDWREMRVRAAMMLLRVGLKVDLDEKVANLSISHKQMLEIAKTL
                     MLDAKVIIMDEPTSSLTNKEVDYLFLIMNQLRKEGTAIVYISHKLAEIRRICDRYTVM
                     KDGSSVCSGMVSDVSNDDIVRLMVGRELQNRFNAMKENVSNLAHDTVFEVRNVTSRDR
                     KKVRDISFSVCRGEILGFAGLVGSGRTELMNCLFGVDKRAGGEIRLNGKDISPRSPLD
                     AVKKGMAYITESRRDNGFFPNFSIAQNMAISRSLKDGGYKGAMGLFHEVDEQRTAENQ
                     RELLALKCHSVNQNITELSGGNQQKVLISKWLCCRPEVIIFDEPTRGIDVGAKAEIYK
                     VMRQLADDGKVILMVSSELPEIITVCDRIAVFCEGRLTQILTNRDDMSEEEIMAWALP
                     QE"
     gene            16823..17803
                     /gene="alsC"
                     /locus_tag="J1Y83_002793"
     CDS             16823..17803
                     /gene="alsC"
                     /locus_tag="J1Y83_002793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-allose ABC transporter permease"
                     /protein_id="HBA7630927.1"
                     /translation="MGFTTRVKSEASEKKPFNFALFWDKYGTFFILAIIVAIFGSLSS
                     EYFLTTNNITQIFVQSSVTVLIGMGEFFAILVAGIDLSVGAILALSGMVTAKLMLAGI
                     DPFLAALIGGVLVGGALGAINGCLVNWTGLHPFIITLGTNAIFRGITLVISDANSVYG
                     FSFDFVNFFAASVIGIPVPVIFSLIVALILWFLTTRMRLGRNIYALGGNKNSAFYSGI
                     DVKFHILVVFIISGVCAGLAGVVSTARLGAAEPLAGMGFETYAIASAIIGGTSFFGGK
                     GRIFSVVIGGLIIGTINNGLNILQVQTYYQLVVMGGLIIAAVALDRLISK"
     gene            17814..18509
                     /gene="rpe"
                     /locus_tag="J1Y83_002794"
     CDS             17814..18509
                     /gene="rpe"
                     /locus_tag="J1Y83_002794"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HBA7630928.1"
                     /translation="MKISPSLMCMDLLKFKEQIEFIDSHADYFHIDIMDGHFVPNLTL
                     SPFFVSQVKKLASKPLDCHLMVTRPQDYIAQLARAGADFITLHPETINGQAFRLIDEI
                     RRHGMKVGLILNPETPVEAMKYYIHKADKITVMTVDPGFAGQPFIPEMLDKVAELKAW
                     REREGLEYEIEVDGSCNQATYEKLMAAGADVFIVGTSGLFNHAENIDEAWRIMTAQIL
                     AAKSEVQPHAKTA"
     gene            18493..19422
                     /gene="alsK"
                     /locus_tag="J1Y83_002795"
     CDS             18493..19422
                     /gene="alsK"
                     /locus_tag="J1Y83_002795"
                     /EC_number="2.7.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="allose kinase"
                     /protein_id="HBA7630929.1"
                     /translation="MQKQHNVVAGVDMGATHIRFCLRTAEGETLHCEKKRTAEVIAPD
                     LVSGIGEMIDEQLRRFNARCHGLVMGFPALVSKDKRTIISTPNLPLTAADLYDLADKL
                     ENTLNCPVEFSRDVNLQLSWDVVENRLTQQLVLAAYLGTGMGFAVWMNGAPWTGAHGV
                     AGELGHIPLGDMTQHCACGNPGCLETNCSGMALRRWYEQQPRNYPLSDLFVHAENAPF
                     VQSLLENAARAIATSINLFDPDAVILGGGVMDMPAFPRETLIAMTQKYLRRPLPYQVV
                     RFIAASSSDFNGAQGAAILAHQRFLLQSCAKVP"
BASE COUNT         4605 a         5384 c         5237 g         4249 t
ORIGIN      
        1 aaataataaa tggaggcaaa aagattattt tctttttgcg tttcctttca aatgaaaacg
       61 atcgtcgtct aaaatcagca gtacccccga caaactcagg gattttgtgt ataattgcgg
      121 cctttttcgg caatctgccg ttttttggcg cttttgccct gctgactttt gaggaaatcc
      181 acatgtcatt accacactgc ccaaaatgca actccgaata cacctacgaa gataacggca
      241 tgtacatctg cccggaatgt gcctacgaat ggaacgacgc agaacctgca caggaaagcg
      301 acgagctgat cgttaaagat gctaacggca atctgctggc tgacggcgac agcgtgacca
      361 tcattaaaga tctgaaggtg aaaggtagct cttcgatgct gaaaattggc accaaagtga
      421 aaaacatccg cctggttgaa ggcgaccata acatcgattg caaaatcgac ggttttggtc
      481 cgatgaaact gaagtctgag tttgtgaaaa agaactgatt gtattgtgat cggtaggccg
      541 gataaggcgc tcgcgccgca tccggcaacg gtggcgactg cctgatgcga cgctgaccgc
      601 gtcttatcag gcttccctct catctgtata aatttcgaac tacacttaac gggcttctct
      661 taactgaggt caccatcatg ccgttaagtc cctacctctc ttttgccggt aactgttccg
      721 acgcgattgc ctattatcaa cgtacgttgg gcgcggaact gctctataaa atcagcttcg
      781 gcgaaatgcc gaaatcagcg caggacagcg ccgaaaactg cccttccgga atgcaatttc
      841 ccgataccgc catcgcgcat gccaacgtgc gcattgccgg aagcgacatc atgatgagcg
      901 atgccatgcc gtcaggaaaa gccagctact ccggctttac gctggtgctc gattcgcaac
      961 aggtggaaga aggaaaacgc tggtttgata accttgccgc caacggaaaa atcgaaatgg
     1021 cctggcagga aactttctgg gcgcatggct ttggcaaagt caccgataaa tttggcgtac
     1081 cgtggatgat taatgtcatc aaacaacagc caacgcaata acccgccggg aggcccgccc
     1141 tcccgcactg tcatcgaatt cccgttaact cttcatctgt tagtcacttt taattaacca
     1201 aatcgtcaca ataatccgcc acgatggagc cactttttta gggaggctgc atcatgcaaa
     1261 cgattatccg tgtcgagaag ctcgccaaaa ccttcaatca gcatcaggcg ctgcacgcgg
     1321 ttgatctgaa cattcatcat ggtgaaatgg tggctctgct tgggccgtcg ggttccggaa
     1381 aatccaccct tttacgtcac ttaagcggtt tgattaccgg cgataaatct gtcggtagcc
     1441 atatcgagct gctgggccgc actgtccagc gcgaaggccg tctggcccgc gatatccgca
     1501 aaagccgcgc ccataccggc tacatctttc aacaattcaa cctggtgaat cgcctgagcg
     1561 tactggagaa cgtgctgatt ggcgcgctcg gcagcacgcc attctggcga acctgtttta
     1621 gctacttcac ccgcgagcag aaacagcgcg cgttacaggc gctgacccgc gttggcatgg
     1681 tgcactttgc ccatcaacgc gtttccacac tctccggcgg ccagcaacag cgtgtggcga
     1741 ttgcccgcgc gctgatgcag caggcgaagg tcattctcgc tgacgaaccc atcgcctcgc
     1801 tggacccgga atcggcacgc atcgtgatgg acaccctgcg cgacatcaac cagaacgacg
     1861 gcattaccgt ggtcgtcacg ctgcatcagg tggattacgc cctgcgctac tgcgaacgca
     1921 tcgtcgccct gcgccagggg cacgtcttct acgacggctg cagccaacag tttgataacg
     1981 aacgttttga ccatctctac cgcagcatta accgcgtcga agagaacgcg aaagctgcct
     2041 gacatcccca tcattgagga aaacgaatga acgctaagat aattgcctcg ctggccttca
     2101 ccagcatgtt cagcctcagc accctgttaa atccggcata cgccgaagag caggaaaagg
     2161 cgctgaattt cggcattatt tcaacggaat cacagcaaaa cctgaaaccg caatggacgc
     2221 cgttcttaca ggatatggag aagaagctgg gcgtaaaggt caacgccttc tttgccccgg
     2281 actacgcggg cattatccag gggatgcgct tcaataaagt ggatatcgcc tggtacggca
     2341 acctgtcggc gatggaagcg gtggatcgcg ccaacggtca ggtcttcgcc cagacggtcg
     2401 cggcggatgg atcgccgggt tactggagcg tgttgatcgt caacaaagat agcccgatca
     2461 acaacctgaa cgatctgctg gcgaagcgga aagatctcac cttcggcaat ggcgatccta
     2521 actctacctc cggcttcctc gtccccggtt actacgtctt cgctaaaaac aatatctccg
     2581 ccagcgactt taagcgcacc gtcaacgccg ggcatgaaac caacgccctc gccgtcgcta
     2641 acaagcaagt ggatgtcgcc accaacaaca ccgaaaacct cgacaagctg aaaacctccg
     2701 cgccggagaa gctgaaagaa ctgaaggtga tctggaaatc accgctgatc cctggcgatc
     2761 cgatcgtctg gcgcaaaaat ctttccgaaa ccactaaaga caagatctac gacttcttta
     2821 tgaattacgg caaaacgccg gaagagaaag cggtgctgga gcgcctgggc tgggcgccgt
     2881 tccgcgcctc cagcgacttg caactggtgc cgattcgcca gctcgcactg tttaaagaga
     2941 tgcaaagcgt gaaggacaac aaagggctga acgaacagga caagctggca aaaaccaccg
     3001 ccattcaggc gcaactggat gatttggacc gcctgaacaa cgcgttaagc gcaatgagtt
     3061 cggtgagtaa agcggtgcag taaatcgtag gtcggataag acgccccggc gtcgcatccg
     3121 acaatgtgca gacgttgatg ccggatgcgg tgcaagcacc ttatccggcc tacagaccgg
     3181 agccaaacat gcaaaccatc accatcgccc cacccaagcg cagctggttc tcgcttctga
     3241 gctgggccgt tgttatcgcc gtgctgatcg cctcgtggca gggcgcggaa atggccccgc
     3301 ttacgctgat taaagatggc ggcaacatgg cgacgttcgc cgccgacttc ttcccgcccg
     3361 atttcagcca gtggcaggat tacctcaccg aaatggccgt cacgctgcaa atcgccgtct
     3421 ggggcaccgc gctggcggtg gttctctcca tccccttcgg cctgatgagc gctgaaaacc
     3481 tggtaccgtg gtgggtctac caacctgtac gccgcctgat ggacgcctgc cgcgccatta
     3541 acgaaatggt cttcgccatg ctgttcgtgg tcgccgtcgg cctcggcccg ttcgctggcg
     3601 tgctggcgct gtttatccac accaccggcg tactttccaa actgctttcc gaagcggtag
     3661 aagcgattga acccggcccg gtggagggca ttcgcgctac cggagccaac aagctggaag
     3721 agatcctcta cggcgtacta ccgcaggtca tgccgctgct gatttcctac tccctctatc
     3781 gcttcgaatc caacgtccgc tcggcgaccg tcgtcggtat ggttggcgcg ggcgggatcg
     3841 gcgtcacgct gtgggaagcg attcgcggtt tccagttcca gcaaacctgc gccctgatgg
     3901 tgctcatcat cgtcacggtc agcctgctgg atttcctctc tcaacggttg cgtaagcact
     3961 ttatctgata agcgaggcat tgatatctat gcacttgtct acacatccga ccagctaccc
     4021 aacacgctat caggagatag ccgcaaaact tgagcaggag cttcgtcaac actaccgctg
     4081 cggcgactat cttcccgccg agcagcaact ggcggcgcgc tttgaggtga accgccacac
     4141 cctgcgccgc gccatcgacc aactggtgga aaaaggctgg gtacagcgcc gtcagggcgt
     4201 cggcgtactg gtgctgatgc gcccgttcga ttacccgctc aacgcccagg cgcgttttag
     4261 ccagaacctg ctggaccagg gcagccatcc caccagcgaa aaattgctat cggtgctgcg
     4321 ccccgcctcc ggtcacgtcg ctgacgcgct ggggataacc gagggggaga acgtcatcca
     4381 cctgcgcacc ctgcgtcggg tcaacggcgt tgcgctctgt ttaatcgacc actacttcgc
     4441 agatctcacg ctctggccaa cgcttcaatg ctttgacagc ggttcgctgc acgattttct
     4501 gcgcgagcaa acggggatta cgctacgccg cagccagacg cggatcagcg cccgccgcgc
     4561 ccaggccaaa gagtgccagc gtcttgaaat cccgaatatg tcgccgctgc tgtgcgtgcg
     4621 cacccttaac caccgtgacg gtgaaagcag cccggcggag tactccgtca gcctgacgcg
     4681 cgccgacatg attgaattca ctatggagca ctgaatgcac gcagataccg cgacccgcca
     4741 gcactggatg tccgtgctgg cgcacagcca accggctgaa ctggcagccc gcctgaacgc
     4801 gctgaacatc aacgccgatt atgaggtgat ccgcgccgct gaaactggcc tggtgcagat
     4861 tcaggcgcga atgggcggca ccggcgaacg cttttttgct ggcgacgcca cgctcacccg
     4921 cgccgccgtg cgtctgactg acggcacgct cggctatagc tgggtgctgg ggcgcgacaa
     4981 acagcacgcc gaacgttgtg cgctgattga tgcgctgatg cagcaaaacc gccactttca
     5041 aaacttatca gaaaccctta ttaccccgct ggacgctgac cgtatggcac gcattgccgc
     5101 gcgtcaggcc gaagtgaacg ccagccgggt cgacttcttt acgatggttc gcggagacaa
     5161 cgcatgaccc tggaaaccgc ttttatgctt cccgtgcagg atgcccagca cagttttcgt
     5221 cgcctgttaa aggccatgag cgagccgggc gtgattgtcg ccctgcatca gctcaagcgc
     5281 ggctggcagc cgctgaatat cgccaccacc agcgtgctgc tgacgctggc cgataacgac
     5341 acgccggtgt ggcttgccgc accattgagc aacgatatcg tcaatcagag cctgcgtttt
     5401 cataccaacg cgtcgctggt taatcagccg gaacaggcga ccttcgcggt aacagatgag
     5461 gcgatctcca gcgagcagct caacgccctt tccaccggca ctgccgttgc accagaagcg
     5521 ggcgcaacgc tgattttaca ggtcaccagc ctgagcggtg ggcgtatgtt gcgcctcacc
     5581 ggcgcaggta ttgccgaaga acggatgatc gccccgcaac tgccaaagtg cattcttcat
     5641 gaacttaccg agcgcccgca cccgttcccg ctcggcatcg acctgatcct gacctgcggc
     5701 gagcgcctgc tggctattcc gcgaaccact catgtggagg tgtgctgatg tacgttgccg
     5761 tgaaaggggg cgaaaaggcg atcgacgccg cccacgccct gcaagagagc cgacgccggg
     5821 gcgatactga tttgcctgaa ctgagcgtcg cccagattga acagcagctt aacctcgcgg
     5881 tagatcgcgt gatgaccgaa ggcggtattg ccgaccgcga gctggccgcg ctggcgctga
     5941 aacaggccag cggcgataac gtcgaagcga tttttctgtt gcgcgcctac cgcaccacgc
     6001 tggcgaagct ggcggtcagt gagccgctcg acaccaccgg gatgcgtctc gaacggcgta
     6061 tttcagcaat ttataaagac attcccggcg gccaactgct tggccccacc tacgactaca
     6121 cccatcgcct gctcgatttc accctgctgg caaacggcga aacgccaacg ctgaccaccg
     6181 ctgacagcga acagcaaccg tcgccacacg tcttcagcct gctggcgcgt caggggctgg
     6241 cgaagtttga agaagatagc ggcgcacaac cggacgacat cacccgcacg ccgccggttt
     6301 atccctgttc gcgttcttcc cgtctacaac aattaatgcg cggtgacgaa ggctatttgt
     6361 tggcgctggc ctactccacc caacgcggtt acgggcgcaa tcacccgttc gcaggcgaaa
     6421 ttcgcagtgg ctatatcgat atatttattg tgccggaaga gctgggattt gcggtgaacg
     6481 tcggcgaatt gttgatgacc gagtgtgaaa tggtcaacgg ttttatcgac ccgccggatg
     6541 aaccaccgca ttttacacgc ggctatgggc tggtgttcgg catgagcgag cgcaaagcaa
     6601 tggcgatggc gctggttgac cgtgcgttgc aggcaccgga atacggcgag cacgcaacag
     6661 gtccggcaca ggatgaagag ttcgtgctgg cccatgccga caacgtcgaa gccgcaggct
     6721 ttgtttcgca tctcaaactc ccgcactacg tcgatttcca ggccgaactg gagctactca
     6781 aacgtctgca acaggagcag aaccatggct aatctgagcg gctacaactt tgcttacctc
     6841 gacgagcaga ccaaacgcat gatccgccgc gctatcttaa aagcggtggc aatccccggt
     6901 tatcaggtgc cgtttggcgg acgcgagatg ccgatgccct acggctgggg aaccggcggc
     6961 attcagctca ccgccagcgt gattggcgaa agcgacgtgt tgaaggtgat tgaccagggc
     7021 gcagacgaca ccaccaacgc cgtgtcgatt cgtaacttct ttaagcgcgt gactggggtg
     7081 aacaccaccg agcgcacgga cgatgcgaca cttatccaga cgcgtcaccg catacccgaa
     7141 acgccgctga ccgaagatca gatagttatc ttccaggtgc cgatccccga accgctgcgc
     7201 tttattgagc cgcgagagac ggaaacgcgc accatgcacg cgctggagga gtacggcgtg
     7261 atgcaggtga aactgtatga agatatcgcc cgcttcggtc atatcgccac cacctacgcc
     7321 tatccggtga aggtgaacgg gcgctacgta atggacccgt cgccgatccc gaaattcgat
     7381 aacccgaaaa tggacatgat gcccgccctg caactgtttg gcgcggggcg cgagaagcgc
     7441 atctatgccg tgccaccgtt tacccgcgtg gaaagcctcg atttcgatga tcatccgttc
     7501 accgtccagc agtgggatga accatgcgcc atctgcggat cgacccacag ctatcttgat
     7561 gaagtggtgc tggatgacgc cggaaaccgc atgtttgtct gctccgatac cgattattgc
     7621 cgccaacaga gcgaggcaaa aaaccaatga atcaaccgtt actttcggtc aataacctga
     7681 cccaccttta tgccccgggc aaaggcttca gcgatgtctc ttttgattta tggccgggag
     7741 aagttctggg cattgttggg gagtccggtt ccgggaagac cacgctactg aaatcaattt
     7801 ccgcccgcct gacaccacag caaggggaaa ttcgctacga gaaccgttcg ctgtacgcga
     7861 tgagcgaggc cgaccgccgt cgcctgctgc gcaccgaatg gggtgtggtg catcagcatc
     7921 cgcttgacgg cctgcgccgc caggtgtcgg caggcggcaa tatcggcgaa cggttgatgg
     7981 caaccggagc gcgtcattac ggcgatattc gcgccaccgc gcagaaatgg ctggaagagg
     8041 tggagattcc cgccaaccgg atcgacgacc tgccgaccac cttttccggc ggcatgcagc
     8101 aacgtttgca gattgcccgc aacctggtga cgcatccgaa gctggtgttt atggatgaac
     8161 ccactggcgg gctggatgtg tcggtacagg cgcgcctgct ggacctgctc cgcggtctgg
     8221 tgatggagct taacctggcg gtggtgattg tcacccacga tttaggcgtc gcccgtctgc
     8281 tggcggaccg tttgctggta atgaagcaag ggaaagtggt ggagagtggg ttgaccgacc
     8341 gggtgctcga cgacccgcac catccgtata cccagttgct ggtgtcatcg gttttgcaga
     8401 attgagctgg tgccggatga ggcgtaaacg ccttatccgg cctacaaatg cgctccccgt
     8461 aggtcggata agacgcatca gcgtcgcatc cgacacccga accacgaggc gaaaaatgat
     8521 taacgtacaa aacgtcagta aaaccttcat cctgcaccag caaaacggcg tgcgcctgcc
     8581 tgtattacaa ggcacctcac tcaccgtcaa cgccggcgaa tgcctggtac tccacggcca
     8641 ttccggcagt ggaaaatcaa ctctgctacg ctcgctgtat gccaactatc tacccgacga
     8701 agggcaaatc cagatcaaac acggtgatga atgggtcgac ctggtcaccg cgccagcgcg
     8761 caaagtggtg gagatccgca aaaccactgt cggctgggta agccagtttc tgcgcgtcat
     8821 cccacgtatt tcggcactgg aagtggtgat gcagccgctg ctcgataccg gcgtaccgcg
     8881 tgaagcctgc gccgctaaag ccgcgcgtct cctcacccac ctgaacgtgc ctgaacgcct
     8941 gtggcacctg gcaccatcga ccttctccgg cggcgaacag cagcgtgtca acatcgcccg
     9001 tggctttatc gtcgactacc ccattctgct gctcgacgaa cctaccgcct cgctggacgc
     9061 taaaaacagc gccgccgtag tggaactgat ccgcgaagcc aaagcccgcg gcgcggccat
     9121 cgtcggtatc ttccatgacg aaaccgtgcg cgagcaagtc gccgaccgcc tgcacccaat
     9181 gggagtctct tcatgattat caataacgtc aaactggtgc tggaaaacga ggtggtacac
     9241 ggttctctgg agatgcagga cggcgaaatc cgcgcctttg ccgaaagcca gagtcacctg
     9301 tcagaagcaa tggacggcga aggcggctgg ctgctaccgg ggctgattga gctgcatacc
     9361 gataatctgg ataaattctt caccccgcgc ccgaaagttg actggccagc ccactcggcg
     9421 atgagcagcc acgacgcgct gatggtggcg agcggcatca ccaccgtgct ggacgccgtg
     9481 gcgattggtg atgtacgcga cggcggcgat cggctggaga atctggagaa gatgatcaac
     9541 gccatcgaag agacgcagaa acgcggcgtc aaccgtgccg agcaccgtct gcacctacgc
     9601 tgcgaactgc cgcatcacac cacgctgccg ctgtttgaaa aactggtaca gcgcgaactg
     9661 gtaacgctgg tgtcgctgat ggatcactca ccgggccagc gccagttcgc caaccgcgaa
     9721 aaatatcgtg aatattatca gggcaaatac tcgctcaccg atgcgcagat gcaacagtac
     9781 gaagaggagc aactggcgct tgccgcacgc tggtcacagc ctaatcgcga aactatcgcc
     9841 gcaatgtgcc gcgcgcgaca cattgccctc gccagccacg atgacgccac gcacgcccac
     9901 gttgccgaat cccaccagct tggcagcgtg atcgccgaat ttcccaccac gttcgaagcg
     9961 gcagaagcct cgcgcaagca tggcatgaac gtgctgatgg gcgcgccgaa tatcgtgcgc
    10021 ggcggttcgc actccggcaa cgtggcggcc agtgaactgg cacagcttgg cctgctggat
    10081 atcctctctt ccgactacta ccccgccagc ctgctcgatg cggcatttcg cgtcgccgat
    10141 gacgagagca accgctttac gctgccgcag gcggtgaagc tggtgactaa aaatccggcg
    10201 caggcgctta atcttcagga tcgcggggtg attggcgagg gcaaacgcgc cgacctggtg
    10261 ctggcgcatc gcaagggcaa ccacattcat atcgaccacg tctggcgtca gggtaaaagg
    10321 gtgttctgat gacgggaaaa ctgatttggt taatgggggc ctctggctcc ggaaaagaca
    10381 gtctgttgac ggaactccgc cagcgggaac aaactcagct actggtagcg catcgctaca
    10441 tcacgcgcgc cgccagcgcc ggaagtgaaa accatatcgc cctgagggag caggagtttt
    10501 ttacccgcgc ggggcagaac cttctggcct taagctggca cgctaacggt ctgtattatg
    10561 gcgtcggcgt cgagattgac ctctggctgc acgctggatt tgacgtggtg gtcaacggct
    10621 cacgcgccca tctgccgcag gcgcgggcgc gctatcaatc ggcgctgctg cccgtttgtt
    10681 tacaggtttc gccagagatc ctgcgccagc gcctggaaaa ccgtggtcgt gaaaatgcca
    10741 gtgaaattaa cgcccggctg gcgcgcgctg cccgctatac tcctcaggat tgtcttacgc
    10801 tcaataatga cggcagcctg cgccagtcgg tcgacaagct gctgacgctg attcatcaga
    10861 aggagaaaca ccatgcctgc ttgtgagctt cgcccggcta cgcagtacga caccgacgcg
    10921 gtttacgcgc tgatttgtga gctaaaacaa gcggagtttg accaccaggc gtttcgcgtg
    10981 ggctttaacg ccaacctgcg tgacccgaat atgcgctacc atctggcgct gttggatggc
    11041 gaggttgtcg gcatgatcgg cctgcatttg cagtttcatc tgcatcatgt caactggatc
    11101 ggcgaaattc aggaactggt ggtgataccg caggcgcgcg gtctgaatgt cggcagtaaa
    11161 ttactggcat gggcagaaga agaagcccgc caggccgggg ccgaaatgac cgagctttcc
    11221 accaacgtga agcgccacga cgcgcaccga ttttacctgc gcgaaggcta cgagcagagc
    11281 cacttccgct tcaccaaggc gctgtaacat gagcctgacc ctcacgctca ccggcaccgg
    11341 cggcgcacag ggcgttccgg catggggctg cgagtgcgcg gcctgcgcca gagcgcgacg
    11401 ctcgccgcag tatcgccgcc agccatgcag cggcgtcgtg aagtttaacg acgcgatcac
    11461 cctgatcgac gccgggctgc acgatctcgc cgatcgctgg tcgcccggat cgttccagca
    11521 gtttttgctg acgcactacc atatggatca cgtccagggg ctgttcccgc tgcgctgggg
    11581 cgtgggcgac acaatcccgg tctacggtcc accggatgag cagggctgcg acgatctgtt
    11641 taaacatccg ggcctgcttg atttcagcca cacggtggag ccgtttgtgg tgtttgattt
    11701 gcaggggttg caggtcacgc cgctgccgct taaccattcg aaactcactt tcggctatct
    11761 actggaaacg gcacatagcc gggtggcgtg gctgtctgac actgcaggcc tgccggaaaa
    11821 aacgctgaaa tttttacgca ataatcagcc acaggtaatg gtgattgatt gcagccaccc
    11881 gccgcgcgag gatgcaccgc gtaatcactg tgatttaaat accgtgcttg cgctgaatca
    11941 ggttatccgc tcgccgcggg tgattctgac ccatatcagc catcagtttg atgcgtggct
    12001 gatggaaaac gcgctaccat cggggtttga ggcggggttt gatgggatgg atattgaggt
    12061 ggcgtgaggg aaaatacccg cgccggcccc tatatcaacg cccatactca tcttccattc
    12121 tccgctcttc atcttccagt tgccgacgct cctgatcaag ctggcgctgg cgatcgtcca
    12181 gctgcctgcg gcgatcttca aactggcggc ggcgatcgtt atattgcttg cgacgatcgt
    12241 cgctcacctg gcgttgccaa ccgtcgtcgc gcgaatcttc atagtctcgc ccacggtcag
    12301 ggttataagc gtcgttaatc gcctgctgaa tattgccaat ggtgtcgtcg ataatatcgg
    12361 cctgggccgg aacggtggac agcgtgagca gggtgaataa aagaaatagc ggaaagcgtt
    12421 tcattagcca acctcaaaaa gaaactctat ccacattagt cattactcat ccatgcaagt
    12481 agtggatgaa tctcaattcc tttgctgctc tattgccgta atcgcctcca cgcgttgttg
    12541 atgacgaccg ccttcgtact gtgcgcccag ccacgcatcg acaatcattt ttgccagttc
    12601 gaggccaacc actcgtgaac caaaagccag cacgttggtg tcgttatgct gccgcgaaag
    12661 ttgcgcggaa taaggttcgc tacaaacgac cgcgcgaatt ccggcaaact tgttcgccgc
    12721 tatcgaaata ccgacgccag tgccacaaat caaaatcccg ccatcagcct ctccgccagc
    12781 aacggccagt gcgacttgac tggcgtaatg cggataatca gtacgctctg acgaccaggt
    12841 tcctttatca atcacttcaa cgccacgctc aactaaatgt gccactattt catgttttaa
    12901 aatgaaaccg acatgatcac agccaaatgc aatctttttc attcatcttc cccgcccgcg
    12961 caaaacttca caattaaaaa acatttgtga tgtaaatgaa ttaaaaacac ccaaaatcga
    13021 tgaaatccgc cttcccgatc tgttttacag agttacatct ggcagcgcaa tagcgttaaa
    13081 gacactcact atccagcaac atctatcatc taataaacca gaaaaacaaa taacatcatg
    13141 tttttaaagt gattaaatga aatgaaattt taagccactc tccattattc acaacaaaac
    13201 aaactttata aattttattt ttttgtgaag ttgccagcat cttttctgtt cttgctgtgg
    13261 tgatatagtg gcgtcttcaa ttcaaggaca agagaacgtg atgagccagt cagagtttga
    13321 ttcagcgctt ccgaacggta tcgggttagc gccttaccta cgaatgaagc aggaaggaat
    13381 gacagaaaac gaaagccgca tcgtggagtg gttactcaaa cccggtaacc tgagttgtgc
    13441 acccgcaatt aaagatgtcg cagaagctct ggcggtatct gaagcgatga tcgttaaggt
    13501 atcaaagctg ctggggttta gcggctttcg taacttacgc agtgcgctgg aagattattt
    13561 ttctcagtca gaacaagtat taccttccga gttggctttt gatgaagcgc cgcaggatgt
    13621 ggtgaataag gtatttaaca tcactttacg caccattatg gaaggtcagt cgatcgtcaa
    13681 cgttgatgag atccaccgtg ccgcccgctt tttctatcag gccagacagc gggatttgta
    13741 cggtgccgga ggatcaaatg ctatctgtgc tgatgtacag cacaagtttt tgcgcattgg
    13801 cgtacgctgc caggcctatc cggacgctca catcatgatg atgtctgctt cgttgttaca
    13861 ggaaggagat gtggtgctgg tggtgactca ttccgggcga accagtgatg taaaagcggc
    13921 tgtagaactg gcaaaaaaga acggggcaaa gattatttgt ataacgcata gctatcattc
    13981 accaatagcg aagctggccg attatattat ttgctcaccg gccccggaaa caccgttatt
    14041 aggtcgtaat gcctcggcaa gaatattaca actgactttg ctggacgcgt tttttgtctc
    14101 tgtcgcccag ctcaacattg aacaagctaa tattaatatg caaaaaaccg gcgcaattgt
    14161 tgatttcttc tcaccaggcg cgctgaaata agaaaaaggt aattccgcca tcgcggagtt
    14221 acctctcccc ttaccgtgag aacatgatta tgaataaata tctgaaatat ttcagcggca
    14281 cactcgtggg cttaatgttg tcaaccagcg cttttgctgc cgccgaatat gccgtcgtat
    14341 tgaaaacact ctccaatcca ttttgggtag atatgaaaaa aggcattgaa gatgaagcaa
    14401 aaacgctggg cgtcagcgtt gatatttttg cctctccttc agaaggcgat tttcaatctc
    14461 aattgcagtt atttgaagat ctcagtaata aaaattacaa aggtatcgcc ttcgcaccat
    14521 tatcctcagt gaacctggtc atgcctgttg cacgcgcatg gaaaaaaggc atttatctgg
    14581 tcaatctcga tgaaaaaatc gacatggata atctgaaaaa agcaggcggt aatgtggaag
    14641 gttttgtcac caccgataat gttgccgtcg gggcgaaagg cgcgtcattc attattgaca
    14701 aattgggcgc tgaaggtggt gaagtcgcaa tcattgaggg taaagccggt aacgcctccg
    14761 gtgaagcgcg tcgtaatggt gccaccaaag ccttcaaaaa agcaagccag atcaagcttg
    14821 tcgccagcca gcctgccgac tgggaccgca ttaaagcact ggatgtcgcc actaacgtat
    14881 tgcaacgtaa tccgaatatt aaagcgatct attgcgcgaa tgacacgatg gcaatgggtg
    14941 ttgctcaggc agtcgcaaac gctggaaaaa cgggcaaagt gttggtcgtc ggtacagatg
    15001 gcattccgga agcccgcaaa atggtggaag ccggacaaat gaccgcgacg gttgcccaga
    15061 acccggcgga tatcggtgca acgggtctga agctgatggt tgacgctgaa aaatccggca
    15121 aggttatccc gctggataaa gcaccggaat ttaaactggt cgattcaatc ctggtcactc
    15181 aataaaactg aatgatgccg gatgatgatg ctaatgaagt gtcttatccg gccaacaaaa
    15241 taacctgaac gtagacctga taaacgtagc catcaggcaa taatacgata tttcgtgcag
    15301 agaggttatt tatggccacg ccatatatat cgatggcggg gatcggcaag tcctttggtc
    15361 cggttcacgc attaaagtcg gttaatttaa cggtttatcc tggtgaaata catgcattac
    15421 taggagaaaa tggtgcgggt aaatctacgc taatgaaagt gttatccggg atacatgagc
    15481 cgaccaaagg caccattacc attaataaca ttaactataa caaactggat cataaattag
    15541 cggcacaact cggtatcggg attatttatc aggaactcag cgttattgat gaattaaccg
    15601 tactggaaaa tttatatatt ggtcgtcatc tgacgaaaaa aatctgtggc gtcaatatta
    15661 tcgactggcg agaaatgcgt gtccgcgccg ccatgatgtt attacgcgtg ggcttgaaag
    15721 ttgatttaga tgagaaagtg gcgaacttat ctatcagcca caagcagatg ctggaaattg
    15781 ccaaaacgct gatgctcgat gccaaagtca tcatcatgga tgaacccacc tcctcactca
    15841 ccaataaaga ggtggactat ctgtttctga tcatgaatca gttgcgtaag gagggtacgg
    15901 ccatcgtcta tatctcgcat aaattggcgg aaattcgccg tatttgcgac cgctatacgg
    15961 tgatgaaaga cggcagcagc gtttgcagcg gcatggtgag cgatgtgtca aatgacgata
    16021 tcgtccgtct gatggtaggc cgcgaattgc aaaaccgttt taacgcgatg aaggagaatg
    16081 tcagcaacct tgcgcacgat acggtttttg aggtgcgcaa cgtcaccagc cgtgacagaa
    16141 aaaaggtccg ggatatctca tttagcgtct gccggggaga aatattaggc tttgccgggc
    16201 tggtcggttc cggacgtaca gagctgatga actgcctgtt tggcgttgat aaacgcgctg
    16261 gcggagaaat ccgtcttaat ggcaaagata tctctccacg ttctcccctg gatgccgtga
    16321 aaaaagggat ggcttacatc actgaaagcc gccgggataa cggctttttc cccaactttt
    16381 ctatcgctca gaacatggct atcagccgca gtctgaaaga cggcggctat aaaggcgcga
    16441 tggggttgtt tcatgaagtt gacgagcaac gtaccgctga aaatcaacgc gaactgctgg
    16501 cgctgaaatg tcattcggta aaccagaaca tcactgaact ctccggcggt aaccagcaga
    16561 aagtcctgat ctccaaatgg ctgtgctgtc gcccggaagt gatcattttc gatgaaccta
    16621 cccgtggcat cgatgttggc gcgaaagccg aaatttacaa agtgatgcgc caactggcgg
    16681 acgacggaaa agtcatcctg atggtgtcat ctgaactacc tgaaattatc accgtctgcg
    16741 accgcatcgc cgtgttctgc gaaggacgac tgacgcaaat cctgaccaat cgcgatgaca
    16801 tgagcgaaga ggagattatg gcatgggctt taccacaaga gtaaaaagcg aagcgagcga
    16861 gaagaaaccg ttcaactttg cgctgttctg ggataaatac ggcacctttt ttatcctggc
    16921 aatcatcgtc gccatttttg gttcgctgtc gtcagaatat tttctgacca ccaataatat
    16981 tacccagatt tttgtgcaaa gctccgtgac ggtattgatc ggcatgggcg agtttttcgc
    17041 tatcctggtc gctggtatcg acctttcggt tggcgcgatt ctggcgcttt ccggtatggt
    17101 gaccgctaaa ctgatgttgg caggtattga tccgtttctc gcggcgctga ttggcggtgt
    17161 actggttggc ggcgcactgg gggcgatcaa cggctgcctg gtcaactgga cgggactaca
    17221 cccgttcatt atcacccttg gcaccaacgc tattttccgt gggatcacgc tggtgatctc
    17281 cgatgccaac tcggtatacg gcttctcatt tgacttcgtg aacttctttg ccgccagcgt
    17341 aattgggata cctgtccccg ttatcttctc gctaattgtc gcgctcatcc tttggtttct
    17401 gacaacgcgt atgcggctcg ggcgcaacat ctacgcactg ggcggcaaca aaaactcggc
    17461 gttctattcc gggattgacg tgaaattcca catcctggtg gtgtttatca tctccggtgt
    17521 ttgtgcaggt ctggcaggcg tcgtctcaac tgcacgactc ggtgccgcag aaccgcttgc
    17581 cggtatgggt tttgaaacct atgccattgc cagcgccatc attggcggca ccagtttctt
    17641 cggcggcaag gggcgcattt tctctgtggt gattggcggg ttgatcatcg gcaccatcaa
    17701 caacggtctg aatattttgc aggtacaaac ctattaccaa ctggtggtga tgggcggatt
    17761 aattatcgcg gctgtcgccc ttgaccgtct tatcagtaag taaggaattg aacatgaaaa
    17821 tctccccctc gttaatgtgt atggatctgc tgaaatttaa agaacagatc gaatttatcg
    17881 acagccatgc cgattacttt cacatcgata tcatggacgg ccactttgtg ccgaatctga
    17941 cgctctcacc gttcttcgta agccaggtaa aaaaactggc gagtaaaccg ctcgactgcc
    18001 atctgatggt aacgcgtccg caggattaca ttgctcaact ggcacgtgcg ggagctgatt
    18061 tcatcacttt gcatccggaa actatcaacg gtcaggcatt ccgcctgatt gatgaaatcc
    18121 gccgtcatgg catgaaagtg gggctgattc ttaatccgga aacgccggtt gaggccatga
    18181 aatactatat ccataaggct gataaaatta cggtcatgac tgtcgatccc ggttttgccg
    18241 gacaaccctt cattcctgaa atgctggata aagttgccga actgaaggca tggcgtgaac
    18301 gagaaggtct ggagtacgaa attgaggtgg acggttcctg caaccaggca acttacgaaa
    18361 aactaatggc ggcaggggcg gatgtcttta tcgtcggcac ttccggcctg tttaatcatg
    18421 cggaaaatat cgacgaagca tggagaatta tgaccgcgca gattctggct gcaaaaagcg
    18481 aggtacagcc tcatgcaaaa acagcataac gtcgtagcgg gcgtggatat gggggcaacg
    18541 catatccgct tttgtctgcg gacagcagaa ggtgaaacgc tacactgcga aaaaaagcgg
    18601 accgcagaag ttattgctcc cgacctggtg tcgggtatcg gcgaaatgat tgacgagcaa
    18661 ctcaggcgct ttaacgctcg ctgtcatggt ctggtgatgg gatttccggc gctggtcagt
    18721 aaagataaac gcaccattat ttctacgcct aacctgccgt taacagcggc agatttatat
    18781 gatctcgccg ataagctcga aaatacgctg aattgtccgg ttgagttttc ccgcgacgtt
    18841 aacctgcaac tttcctggga cgtagtagaa aaccgcctta cgcaacaact ggttctggca
    18901 gcctatctcg gtacggggat ggggttcgca gtgtggatga acggtgcgcc gtggacgggt
    18961 gcacacggtg tggcaggcga actgggccat atccccctgg gagatatgac ccaacactgc
    19021 gcgtgtggta atcctgggtg cctggaaacc aattgctctg gaatggcgct aagacgctgg
    19081 tacgaacaac agccccgaaa ttacccattg agcgatcttt tcgtccatgc ggaaaacgcc
    19141 cctttcgtcc agagtctgct tgaaaacgcg gcacgagcca ttgccaccag cattaatctg
    19201 ttcgatcccg atgcggtgat tctgggtggt ggcgtgatgg atatgcccgc cttcccacgc
    19261 gagactctca ttgccatgac ccaaaagtac ctgcgccgtc cactgccgta tcaggtcgtg
    19321 cgctttattg ccgcctcatc ttctgacttt aatggcgctc agggtgcagc aatattggcg
    19381 catcaacgtt ttttgctaca gtcctgtgct aaagtcccat gagtaaagcg ttggcacagt
    19441 agcgcaatat cccgctgtgc caacgacagg tacaa
//
LOCUS       DADSYM010000092        19248 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2415
            SAMN08039579-rid11723173.denovo.092, whole genome shotgun sequence.
ACCESSION   DADSYM010000092 DADSYM010000000
VERSION     DADSYM010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039579
            Sequence Read Archive: SRR6362533
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 19248)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19248)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980162.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 03:39:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,484
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 4,347
            CDSs (with protein)               :: 4,347
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 43 of 85
            Pseudo Genes (incomplete)         :: 37 of 85
            Pseudo Genes (internal stop)      :: 19 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19248
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039579-rid11723173.denovo.092"
                     /strain="LGE2415"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8302 N 95.9628 W"
                     /collection_date="2015-08-24"
     gene            2..904
                     /locus_tag="J1Y83_002796"
     CDS             2..904
                     /locus_tag="J1Y83_002796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBA7630930.1"
                     /translation="MANLYDLKKFDLNLLVIFECIYQHLSISKAAESLYITPSAVSQS
                     LQRLRAQFNDPLFIRSGKGIAPTTTGLNLHHHLEKNLRGLEQTINIVNKSELKKNFII
                     YGPQLISCTNNNMLIRCLRQDTSIDIECHDILLSAENAEELLVQRKTDLAITLQPVIS
                     RSVICMPLHTIRNSLICSNKHPRITDTSNYEQIIAEEFTLLISKSAGIDEIQMDIDER
                     FINRKVSFRSSSLLTIINSISVTDLLGIVPTELYDLHRDFLKLKEIKLEQPLPAVKLY
                     ISYNKASLNNLVFSRFIDRLNDSF"
     gene            1051..2271
                     /locus_tag="J1Y83_002797"
     CDS             1051..2271
                     /locus_tag="J1Y83_002797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase"
                     /protein_id="HBA7630931.1"
                     /translation="MSIYKIPLPLNILEAAKERITWTLNTLPRICVSFSGGKDSGLML
                     HLTAEIARQMGKKICVLFIDWEAQFSCTINYVQSLREFYADVIEEFYWVALPLTTQNS
                     LSQYQPEWQCWEPDVEWVRQPPQDAITDPDFFSFYQPGMTFEQFVREFAEWFSQKRPA
                     AMMIGIRADESFNRFVAIASLNKQRFADDKPWTTAAPGGHSWYIYPIYDWKVADIWTW
                     YANHQQLCNPLYNIMYQAGVPLRHMRICEPFGPEQRQGLWLYHVIEPDRWAAMCARVS
                     GVKSGGIYAGHDNHFYGHRKILKPEHLDWQEYALLLLNSMPEKTAEHYRNKIAIYLHW
                     YQKKGIEVPQTQQGDIGAKDIPSWRRICKVLLNNDYWCRALSFSPTKAKNYQRYNERI
                     KGKRQEWGILCNND"
     gene            2244..2874
                     /locus_tag="J1Y83_002798"
                     /pseudo
     CDS             2244..2874
                     /locus_tag="J1Y83_002798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308667.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ParB-like nuclease domain-containing protein"
     gene            complement(2875..4035)
                     /gene="ybdL"
                     /locus_tag="J1Y83_002799"
     CDS             complement(2875..4035)
                     /gene="ybdL"
                     /locus_tag="J1Y83_002799"
                     /EC_number="2.6.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine-oxo-acid transaminase"
                     /protein_id="HBA7630932.1"
                     /translation="MTNNPLIPQSKLPQLGTTIFTRMSALAQQHQAINLSQGFPDFDG
                     PRYLQERLAYHVAQGANQYAPMTGVQALREAIAQKTERLYGYQPDVDSNITVTAGATE
                     ALYAAITALVRNGDEVICFDPSYDSYAPAIALSGGIVKRIALQPPHFRVDWQEFAALL
                     SERTRLVILNTPHNPSATVWQQTDFAALWQAIAGHEIFVISDEVYEHINFSQQGHASV
                     LAHPQLRERAVAVSSFGKTYHMTGWKVGYCVAPAPISAEIRKVHQYLTFSVNTPAQLA
                     LADMLRAEPEHYLALPDFYRQKRDILVNALNESRLEILPCEGTYFLLVDYSAVSTLDD
                     VEFCQWLTREHGVAAIPLSVFCADPFPHKLIRLCFAKKESTLLAAAERLRQL"
     gene            4144..5232
                     /gene="hcxA"
                     /locus_tag="J1Y83_002800"
     CDS             4144..5232
                     /gene="hcxA"
                     /locus_tag="J1Y83_002800"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxycarboxylate dehydrogenase HcxA"
                     /protein_id="HBA7630933.1"
                     /translation="MPHNPIRVVVGPANYFSHPGSFNHLHDFFTDEQLSRAVWIYGER
                     AIAAAQTKLPPAFELPGVKHILFRGHCSESDVQQLAAESGDDRSVVIGVGGGALLDTA
                     KALARRLGLPFVAVPTIAATCAAWTPLSVWYNDAGQALHYEIFDDANFMVLVEPEIIL
                     NAPQEYLLAGIGDTLAKWYEAVVLAPQPETLPLTVRLGINNAQAIRDVLLNSSEQALA
                     DQQNQQLTQSFCDVVDAIIAGGGMVGGLGDRFTRVAAAHAVHNGLTVLPQTEKFLHGT
                     KVAYGILVQSALLGQDDVLAQLTGAYQRFHLPTTLAELEVDINNQVEIDKMIAHTLRP
                     VESIHYLPVTLTPDTLRAAFEKVESFKA"
     gene            complement(5242..5439)
                     /locus_tag="J1Y83_002801"
     CDS             complement(5242..5439)
                     /locus_tag="J1Y83_002801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbdD/YjiX family protein"
                     /protein_id="HBA7630934.1"
                     /translation="MFDSLAKAGKYLGQAAKLMIGMPDYDNYVEHMRVNHPDQTPMTY
                     EEFFRERQDARYGGKGGARCC"
     gene            complement(5552..7657)
                     /gene="cstA"
                     /locus_tag="J1Y83_002802"
     CDS             complement(5552..7657)
                     /gene="cstA"
                     /locus_tag="J1Y83_002802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation protein CstA"
                     /protein_id="HBA7630935.1"
                     /translation="MNKSGKYLVWTVLSVMGAFALGYIALNRGEQINALWIVVASVCI
                     YLIAYRFYGLYIAKNVLAVDPTRMTPAVRHNDGLDYVPTDKKVLFGHHFAAIAGAGPL
                     VGPVLAAQMGYLPGMIWLLAGVVLAGAVQDFMVLFVSTRRDGRSLGELVKEEMGPTAG
                     VIALVACFMIMVIILAVLAMIVVKALTHSPWGTYTVAFTIPLALFMGIYLRYLRPGRI
                     GEVSVIGLVFLIFAIISGGWVAESPTWAPYFDFTGVQLTWMLVGYGFVAAVLPVWLLL
                     APRDYLSTFLKIGTIVGLAVGILIMRPTLTMPALTKFVDGTGPVWTGNLFPFLFITIA
                     CGAVSGFHALISSGTTPKMLANEGQACFIGYGGMLMESFVAIMALVSACIIDPGVYFA
                     MNSPMAVLAPAGTADVVASAAQVVSSWGFAITPDTLNQIASEVGEQSIISRAGGAPTL
                     AVGMAYILHGALGGMMDVAFWYHFAILFEALFILTAVDAGTRAARFMLQDLLGVVSPG
                     LKRTDSLPANLLATALCVLAWGYFLHQGVVDPLGGINTLWPLFGIANQMLAGMALMLC
                     AVVLFKMKRQRYAWVALVPTAWLLICTLTAGWQKAFSPDAKVGFLAIANKFQAMIDSG
                     NIPSQYTESQLAQLVFNNRLDAGLTIFFMVVVVVLALFSIKTALAALKEPKPTAKETP
                     YEPMPENVEEIVAQAKGAH"
     gene            complement(7838..8251)
                     /gene="entH"
                     /locus_tag="J1Y83_002803"
     CDS             complement(7838..8251)
                     /gene="entH"
                     /locus_tag="J1Y83_002803"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proofreading thioesterase EntH"
                     /protein_id="HBA7630936.1"
                     /translation="MIWKRHLTLDELNATSDNTMVAHLGIVYTRLGDDVLEAEMPVDT
                     RTHQPFGLLHGGASAALAETLGSMAGFMMTRDGQCVVGTELNATHHRPVSEGKVRGVC
                     QPLHLGRQNQSWEIVVFDEQGRRCCTCRLGTAVLG"
     gene            complement(8254..9000)
                     /gene="dhbA"
                     /locus_tag="J1Y83_002804"
     CDS             complement(8254..9000)
                     /gene="dhbA"
                     /locus_tag="J1Y83_002804"
                     /EC_number="1.3.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase"
                     /protein_id="HBA7630937.1"
                     /translation="MDFSGKNVWVTGAGKGIGYATALAFVEAGAKVTGFDQAFTQEQY
                     PFATEVMDVADAAQVAQVCQRLLAETERLDVLVNAAGILRMGATDQLSKEDWQQTFAV
                     NVGGAFNLFQQTMNQFRRQRGGAIVTVASDAAHTPRIGMSAYGASKAALKSLALSVGL
                     ELAGSGVRCNVVSPGSTDTDMQRTLWVSDDAEEQRIRGFGEQFKLGIPLGKIARPQEI
                     ANTILFLASDLASHITLQDIVVDGGSTLGA"
     gene            complement(9000..9857)
                     /gene="entB"
                     /locus_tag="J1Y83_002805"
     CDS             complement(9000..9857)
                     /gene="entB"
                     /locus_tag="J1Y83_002805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin biosynthesis bifunctional
                     isochorismatase/aryl carrier protein EntB"
                     /protein_id="HBA7630938.1"
                     /translation="MAIPKLQAYALPESHDIPQNKVDWAFEPQRAALLIHDMQDYFVS
                     FWGENCPMMEQVIANIAALRDYCKQHNIPVYYTAQPKEQSDEDRALLNDMWGPGLTRS
                     PEQQKVVDRLTPDADDTVLVKWRYSAFHRSPLEQMLKESGRNQLIITGVYAHIGCMTT
                     ATDAFMRDIKPFMVADALADFSRDEHLMSLKYVAGRSGRVVMTEELLPAPIPASKAEL
                     REVILPLLDESDEPFDDDNLIDYGLDSVRMMALAARWRKVHGDIDFVMLAKNPTIDAW
                     WKLLSREVK"
     gene            complement(9871..11481)
                     /gene="entE"
                     /locus_tag="J1Y83_002806"
     CDS             complement(9871..11481)
                     /gene="entE"
                     /locus_tag="J1Y83_002806"
                     /EC_number="2.7.7.58"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2,3-dihydroxybenzoyl)adenylate synthase"
                     /protein_id="HBA7630939.1"
                     /translation="MSIPFTRWPEEFARRYREKGYWQDLPLTDILTRHVASDSIAVID
                     GERQLSYRELNQAADNLACSLRRQGIKPGETALVQLGNVAELYITFFALLKLGVAPVL
                     ALFSHQRSELNAYASQIEPALLIADRQHALFSGDDFLNTFVAEHSSIRMVQLLNDSGE
                     HNLQDAINHPAEDFTATPSPADEVAYFQLSGGTTGTPKLIPRTHNDYYYSVRRSVEIC
                     QFTQQTRYLCAIPAAHNYAMSSPGSLGVFLAGGTVVLAADPSATLCFPLIEKHQVNVT
                     ALVPPAVSLWLQTLAEGESRAQLASLKLLQVGGARLSATLAARIPAEIGCQLQQVFGM
                     AEGLVNYTRLDDSAEKIIHTQGYPMCPDDEVWVADAEGNPLPQGEVGRLMTRGPYTFR
                     GYYKSPQHNASAFDANGFYCSGDLISIDPEGYITVQGREKDQINRGGEKIAAEEIENL
                     LLRHPAVIYAALVSMEDELMGEKSCAYLVVKEPLRAVQVRRFLREQGIAEFKLPDRVE
                     CVDSLPLTAVGKVDKKQLRQWLASRTSA"
     gene            complement(11491..12666)
                     /gene="entC"
                     /locus_tag="J1Y83_002807"
     CDS             complement(11491..12666)
                     /gene="entC"
                     /locus_tag="J1Y83_002807"
                     /EC_number="5.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate synthase EntC"
                     /protein_id="HBA7630940.1"
                     /translation="MDTSLAEEVQQTMATLAPNRFFFMSPYRSFTTSGCFARFDEPAV
                     NGDSPDSPFQQKLAALFADAKAQGIKNPVMVGAIPFDPRQPSSLYIPESWQSFSRQEK
                     QASARRFTRSQSLNVVERQAIPEQTTFEQMVARAAALTATPQVDKVVLSRLIDITTDA
                     AIDSGVLLERLIAQNPVSYNFHVPLADGGVLLGASPELLLRKDGERFSSIPLAGSARR
                     QPDEVLDREAGNRLLASEKDRHEHELVTQAMKEVLRKRSSELHVPSSPQLITTPTLWH
                     LATPFEGKANSQENALTLACLLHPTPALSGFPHQAATQVIAELEPFDRELFGGIVGWC
                     DSEGNGEWVVTIRCAKLRENQVRLFAGAGIVPASSPLGEWRETGVKLSTMLNVFGLH"
     gene            12855..13811
                     /gene="fepB"
                     /locus_tag="J1Y83_002808"
     CDS             12855..13811
                     /gene="fepB"
                     /locus_tag="J1Y83_002808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe2+-enterobactin ABC transporter
                     substrate-binding protein"
                     /protein_id="HBA7630941.1"
                     /translation="MRLAPLYRNALLLTGLLLSGIAAVQAADWPRQITDSRGTHTLES
                     QPQRIVSTSVTLTGSLLAIDAPVIASGATTPNNRVADDQGFLRQWSKVAKERKLQRLY
                     IGEPSAEAVAAQMPDLILISATGGDSALALYDQLSTIAPTLIINYDDKSWQSLLTQLG
                     EITGHEKQAAERIAQFDKQLAAAKEQIKLPPQPVTAIVYTAAAHSANLWTPESAQGQM
                     LEQLGFTLAKLPAGLNASQSQGKRHDIIQLGGENLAAGLNGESLFLFAGDQKDANAIY
                     ANPLLAHLPAVQNKQVYALGTETFRLDYYSAMQVLERLKALF"
     gene            complement(13815..15065)
                     /gene="entS"
                     /locus_tag="J1Y83_002809"
     CDS             complement(13815..15065)
                     /gene="entS"
                     /locus_tag="J1Y83_002809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin transporter EntS"
                     /protein_id="HBA7630942.1"
                     /translation="MNKQSWLLNLSLLKTHPAFRAVFLARFISIVSLGLLGVAVPVQI
                     QMMTHSTWQVGLSVTLTGGAMFVGLMVGGVLADRYERKKVILLARGTCGIGFIGLCLN
                     ALLPEPSLLAIYLLGLWDGFFASLGVTALLAATPALVGRENLMQAGAITMLTVRLGSV
                     ISPMIGGLLLATGGVAWNYGLAAAGTFITLLPLLSLPALPPPPQPREHPLKSLLAGFR
                     FLLASPLVGGIALLGGLLTMASAVRVLYPALADNWQMSAAQIGFLYAAIPLGAAIGAL
                     TSGKLAHSVRPGLLMLLSTLGAFLAIGLFGLMPMWILGVVCLALFGWLSAVSSLLQYT
                     MLQTQTPEAMLGRINGLWTAQNVTGDAMGAALLGGLGAMMTPVASASASGFGLLIIGV
                     LLLLVLVELRRFRQTPPQVTASDS"
     gene            15176..16180
                     /gene="fepD"
                     /locus_tag="J1Y83_002810"
     CDS             15176..16180
                     /gene="fepD"
                     /locus_tag="J1Y83_002810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-siderophore ABC transporter permease"
                     /protein_id="HBA7630943.1"
                     /translation="MSGSVAVTRAIAVPGLLLLLIIATALSLLIGAKSLPASVVLEAL
                     SGTCQSADCTIVLDARLPRTLAGLLAGGALGLAGALMQTLTRNPLADPGLLGVNAGAS
                     FAIVLGAALFGYSSAQEQLAMAFAGALVASLIVAFTGSQGGGQLSPVRLTLAGVALAA
                     VLEGLTSGIALLNPDVYDQLRFWQAGSLDIRNLHTLKVVLIPVLTAGATALLLSRALN
                     SLSLGSDTATALGSRVARTQLIGLLAITVLCGSATAVVGPIAFIGLMMPHMARWLVGA
                     DHRWSLPVTLLATPALLLFADIIGRVIVPGELRVSVVSAFIGAPVLIFLVRRKTRGGA
                     "
     gene            16177..17169
                     /gene="fepG"
                     /locus_tag="J1Y83_002811"
     CDS             16177..17169
                     /gene="fepG"
                     /locus_tag="J1Y83_002811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-enterobactin ABC transporter permease"
                     /protein_id="HBA7630944.1"
                     /translation="MIYVSRRLIITCLLLLIACVMAGVWGLRSGAVTLETSQVFAALM
                     GDTPRSMTMVVTEWRLPRVLMALLIGAALGVSGAIFQSLMRNPLGSPDVMGFNTGAWS
                     GVLVAMVLFGQDLTAIALAAMAGGIVTSLLVWLLAWRNGIDTFRLIIIGIGVRAMLVA
                     FNTWLLLKASLETALTAGLWNAGSLNGLTWAKTSPSAPIIILMLIATALLVRRMRLLE
                     MGDDTACALGVSVERSRLLMMLVAVVLTAAATALAGPISFIALVAPHIARRISGTARW
                     GLTQAALCGALLLLAADLCAQQLFMPYQLPVGVVTVSLGGIYLIVLLIQESRKK"
     gene            17166..17981
                     /gene="fepC"
                     /locus_tag="J1Y83_002812"
     CDS             17166..17981
                     /gene="fepC"
                     /locus_tag="J1Y83_002812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001601847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-enterobactin ABC transporter ATP-binding
                     protein"
                     /protein_id="HBA7630945.1"
                     /translation="MTESVARLRGEQLTLGYGKYTVAENLTVEIPDGHFTVIIGPNGC
                     GKSTLLRTLSRLMTPAHGHVWLDGEHIQHYASKEVARRIGLLAQNATTPGDITVQELV
                     ARGRYPHQPLFTRWRKEDEEAVTKAMQATGITHLADQSVDTLSGGQRQRAWIAMVLAQ
                     ETAIMLLDEPTTWLDISHQIDLLELLSELNREKGYTLAAVLHDLNQACRYASHLIALR
                     EGKIVAQGAPKEIVTAELIERIYGLRCMIIDDPVAGTPLVVPLGRTAPSTAKI"
     gene            complement(17978..19111)
                     /gene="fepE"
                     /locus_tag="J1Y83_002813"
     CDS             complement(17978..19111)
                     /gene="fepE"
                     /locus_tag="J1Y83_002813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS O-antigen length regulator"
                     /protein_id="HBA7630946.1"
                     /translation="MSSLNIKQGSEAHFPEYPLASPSNNEIDLLSLIEVLWRAKKTVM
                     AVVFAFACAGLLISFILPQKWTSSAVITPAEAIQWQDLEKTFTKLRVLDLDVNIDRGG
                     AFNLFIKKFQSVSLLEEYLRSSPYVMDQLKEAKIDELDLHRAIVALSEKMKAVDDNAS
                     KKKDEPSLYTSWTLSFTAPTSEEAQTVLSGYIDYISVLVVKESIENVRNKLEIKTQFE
                     KAKLAQDRIKMKNQLDANIQRLNYSLDIANAAGIKKPVYSNGQAVKDDPDFSISLGAD
                     GIERKLEIEKAVTDVAELNGELRNRQYLVEQLTKANINDVNFTPFKYQLRPSLPVKKD
                     GPGKAIIVILSALIGGMVACGGVLLRHAMASRKQDAMMADHLV"
BASE COUNT         4640 a         5368 c         4838 g         4402 t
ORIGIN      
        1 tatggctaat ctttatgatc taaaaaaatt tgacctcaat ttactggtca tatttgaatg
       61 tatttatcaa catctcagca ttagtaaagc ggcagaatca ttatatataa cgccctctgc
      121 tgttagtcag tcgttacaac gtttacgtgc gcaatttaat gacccgttat tcattcgctc
      181 agggaaaggt atcgcgccga ctacaacagg gttaaacctg catcaccatc ttgagaaaaa
      241 tctcagaggc cttgaacaaa cgatcaatat cgttaataag tctgaactaa agaaaaattt
      301 tattatctac ggtcctcagc ttatttcttg tactaataac aacatgctaa tccgctgtct
      361 tagacaagat acttcaatcg atatagaatg ccatgacatc ctgctttcag ctgaaaatgc
      421 cgaagaactg ttggttcaac gcaaaactga cctggcaatt acgctgcaac cagtaattag
      481 tcgctctgtc atctgtatgc cgttacacac tattcgcaat tcccttatat gtagcaacaa
      541 acatcctcgt attactgata ccagtaacta tgaacaaatt atcgccgaag aattcaccct
      601 gttgatatct aaatcagcag ggattgacga aattcaaatg gacatagatg aacgttttat
      661 aaaccgtaaa gtttcatttc gcagttcttc tttgttgact ataattaata gcatttctgt
      721 tactgactta ttaggcattg tgcccaccga actgtacgat ttacatcgtg actttttgaa
      781 gttaaaagaa ataaaactgg aacaacctct tcctgcggtt aaactttata tttcctataa
      841 caaggcttcg ttgaacaacc ttgtcttctc ccgattcata gatcgtttaa atgatagctt
      901 ctgattcatt accactgaca aataatatat tacgcatcaa ttaattcatt gtgtgaatat
      961 aaaataatgt gctctatctt gcttcgttta tcctataatt agcggattca tctcacgaat
     1021 caccccgtaa cgttcagaga gcgtttttag atgtctattt ataaaatccc gcttccactg
     1081 aatattctgg aagctgcaaa agaacgtatt acctggacac ttaataccct accccgtatc
     1141 tgtgtttctt tttcgggtgg taaagattcc ggattaatgc tgcatctgac cgccgaaatt
     1201 gcccgacaaa tgggcaaaaa aatctgcgtt ttgtttatcg actgggaagc gcagttctca
     1261 tgcactatta actatgttca gtcattacgt gagttttacg ccgatgtcat cgaagagttt
     1321 tactgggttg cgctcccgct tacgacgcaa aattcccttt ctcaatacca acccgaatgg
     1381 cagtgctggg aacctgatgt cgaatgggtg cgtcagcccc cgcaagatgc gataaccgat
     1441 cctgactttt tctcctttta ccagcctggc atgaccttcg aacaatttgt gcgtgagttt
     1501 gctgaatggt tttcacaaaa acgtccggcg gcgatgatga tcggcatccg tgcagatgag
     1561 tccttcaacc gttttgtcgc catcgccagt ttaaataaac aacgttttgc cgatgataag
     1621 ccctggacta ctgccgcacc aggcggtcat agctggtaca tttaccccat ctacgactgg
     1681 aaagtggctg atatctggac ctggtatgct aaccatcaac agctctgtaa cccactgtat
     1741 aatataatgt atcaggcagg cgtccctctg cgtcatatgc gaatttgcga accttttggc
     1801 ccggagcaac ggcaaggatt atggctctat cacgttatcg aaccggatcg ctgggctgct
     1861 atgtgcgcac gagtcagcgg ggtaaaaagt ggcggcattt acgccggaca tgataatcat
     1921 ttttatggtc atcggaaaat cctcaagcca gaacatttag actggcaaga atatgcatta
     1981 ttgctgctca atagcatgcc ggaaaaaacg gctgagcatt accgcaataa aatcgctatt
     2041 tatttgcact ggtatcagaa aaaaggcatc gaagttccac aaacccagca aggggacatt
     2101 ggcgcgaaag atatcccctc ctggcggcga atatgcaaag ttttactcaa taacgattac
     2161 tggtgccggg cattatcatt tagtcctacg aaagcgaaga actatcagcg ttataacgaa
     2221 cggataaaag gaaaacgtca ggaatggggg atactatgca acaacgatta acgcaggatt
     2281 taacccaatt tctcgccagc ttgccggaag atgatcgcat caaagcgatt aatgaaatcc
     2341 gcatggcgat ccatcaggtt agcccctttc gtgaagagcc agtggattgc gttctgtggg
     2401 ttaaaaacag ccaacttatg cctaatgatt acaacccaaa caacgtggca ccacccgaaa
     2461 aaaaaactgc tgaaaaaatc cattgaaata gatggtttta cccaacccat tgtggtcacg
     2521 catacggata aaaatgctct ggaaatcgtg gatggttttc accgccatga aattggtaaa
     2581 ggctcatcat cgttaaaatt acgcttaaaa ggttatttgc cggtgacctg tctggaaggt
     2641 acgcgtaatc aacgcatcgc cgcgactatt cgtcataatc gcgctcgtgg acgtcaccaa
     2701 atcactgcca tgtcagaaat cgtccgagaa ctcagccagt tgggatggga cgataataaa
     2761 attggcaaag agctgggaat ggacagcgac gaagtgttgc gcctgaagca aattaacggc
     2821 ctgcaagagt tgtttgccga ccgtcaattt tcccgcgcct ggacggttaa ataactacag
     2881 ctggcgcagg cgttctgctg ctgccagcaa cgtcgattcc ttcttggcaa aacagagacg
     2941 aatcagttta tgtgggaagg gatcggcgca aaacaccgac agcggaatcg ccgctacgcc
     3001 gtgctcccgc gtcagccact ggcaaaactc aacatcatcc agcgtagata cggcgctgta
     3061 atccaccaac aaaaagtatg taccttcgca cggtaaaatc tccagccgac tttcatttaa
     3121 ggcattcacc agaatatcgc gcttctggcg ataaaagtcc ggtaacgcaa gataatgctc
     3181 aggttctgca cgtagcatat cggcaagcgc cagttgtgcc ggggtattca ccgaaaaggt
     3241 cagatactga tgcactttgc ggatctcggc gctgatgggc gcaggcgcaa cgcaataccc
     3301 cactttccag ccggtcatat gataggtctt gccaaacgat gacaccgcca ccgctcgctc
     3361 acgcaattgc ggatgcgcca gcacgctggc atggccctgt tgtgaaaaat tgatgtgctc
     3421 gtagacttca tcgctaatga caaaaatctc gtgcccggcg atcgcctgcc acagagcggc
     3481 gaaatcagtc tgctgccaga cagttgcact ggggttatgc ggggtgttga ggatcaccag
     3541 tcgggtgcgc tcgcttaaca atgcggcaaa ttcctgccag tcaacgcgaa aatgcggcgg
     3601 ttgcagtgca atacgtttca ctattccccc cgaaagtgcg atggcggggg cgtaactgtc
     3661 atagctggga tcaaaacaaa tcacttcgtc gccattgcgc accaatgcgg ttatcgccgc
     3721 gtataacgcc tccgtcgccc ctgccgtcac ggtgatattg ctgtcgacat ccggttgata
     3781 gccatacaga cgttccgttt tctgagcaat cgcctcgcgc aaggcctgca cgccggtcat
     3841 gggtgcgtat tggtttgccc cctgggcaac gtggtacgcc agccgctcct gtaaatagcg
     3901 cggaccatca aaatcaggaa agccttgcga aaggttaatc gcctggtgtt gctgcgccag
     3961 cgcgctcatc cgggtgaaaa tagtggtgcc aagttgtgga agtttgcttt gtggaatcag
     4021 agggttattt gtcattttta ttgtacctga cgaagttgcg tgttgaaggc actataacac
     4081 gatgctagta tttggcaatc aagacgttta gatgtctaaa taaaacaata aggacaacac
     4141 aacatgcctc acaatcctat ccgcgtggtc gtcggcccgg ctaactactt ttcacatccc
     4201 ggaagtttca atcacctgca cgattttttc actgatgaac aactttctcg cgcggtgtgg
     4261 atctacggcg aacgcgccat tgctgcggca caaaccaaac tgccgcctgc gtttgaactg
     4321 ccaggggtaa agcatatttt gtttcgtggt cattgcagcg aaagcgatgt gcaacaactg
     4381 gcggcggagt ccggtgacga tcgcagcgtg gtgattggcg tcggcggcgg cgcactactc
     4441 gacaccgcga aagccctcgc ccgccgtctc ggtctgccgt ttgttgccgt tccgacgatc
     4501 gccgctacct gcgccgcctg gacaccgctc tccgtctggt ataacgatgc cggacaagcg
     4561 ctgcattatg agattttcga cgacgccaat tttatggtgc tggtggaacc ggagattatc
     4621 ctcaatgcgc cacaagaata tctactggcg gggatcggtg acacgctggc gaaatggtat
     4681 gaagcggtgg tgctggcccc gcaaccagaa acgttgccgc taaccgtgcg gctagggatc
     4741 aataatgcgc aggccattcg cgacgtcttg ttaaacagta gcgaacaggc gcttgccgat
     4801 cagcaaaatc agcaattaac gcaatcattt tgcgatgtgg tggatgccat tattgctggt
     4861 ggtgggatgg tcggtggtct gggcgatcgt tttacgcgtg tagcggcggc ccatgccgtg
     4921 cataacggtc tgaccgtttt gccacaaacc gagaagtttc tccacggcac caaagtcgcc
     4981 tacggaattc tggtgcaaag cgccttgctg ggtcaggatg atgtgctggc gcaattaacc
     5041 ggggcgtatc agcgttttca tctgccgact acgctggcgg agctggaagt ggatatcaat
     5101 aatcaggtgg agatcgacaa aatgattgcc cacaccctac gtccggtgga atccattcat
     5161 tacctgccag tcacgctgac accagatacc ttgcgtgcag cgttcgaaaa agtggaatcg
     5221 tttaaagcct gacgtacaaa tttagcagca gcgcgcgccg ccttttccac cgtagcgcgc
     5281 gtcctgccgc tcacggaaaa actcctcgta ggtcatcggc gtttgatcgg gatggttaac
     5341 ccgcatatgt tcgacatagt tgtcgtaatc aggcatacca atcatcagtt tcgccgcctg
     5401 acctaaatat tttccggctt ttgccagtga atcaaacatc taaacctccg gtaatacaaa
     5461 tgccctcctg aattaagagg gcttaattgc tatcaacacg ctcgggctga gagtaagggg
     5521 aacgctcagc acatcccccc tcactctgac tttagtgcgc gccttttgcc tgcgccacga
     5581 tctcctcgac attttccggc atcggctcat atggcgtttc tttcgccgtt ggcttcggct
     5641 ctttcaatgc cgccagtgcc gtcttaatcg agaacagcgc cagaaccacc acaaccacca
     5701 taaagaagat ggttaatccg gcatccagac ggttgttgaa caccagttgc gccagttgtg
     5761 actcagtata ctgcgacgga atattgccgc tgtcgatcat tgcctggaac ttgttagcaa
     5821 tcgccaggaa tccgactttc gcatccgggc taaacgcttt ctgccagcct gcggtcaggg
     5881 tacaaatcag cagccaggcc gttggtacca gcgccaccca ggcgtaacgt tgacgtttca
     5941 ttttgaacag caccacagca cagagcatca gcgccatccc tgccagcatc tggttggcaa
     6001 taccaaacag cggccacaag gtgttaatac cgcccagcgg atcgaccacg ccctgatgca
     6061 ggaagtagcc ccacgccagc acgcacagcg ccgttgccag caggttagca ggcagtgaat
     6121 cggtccgttt caggcccgga gacaccacgc ccagcagatc ctgcaacata aagcgcgcag
     6181 cacgcgtacc cgcatccacc gccgtaagaa taaacagtgc ttcaaacaga atggcgaagt
     6241 gataccagaa cgccacatcc atcatgccgc ccagcgcacc gtgcagaatg taggccatcc
     6301 ccaccgccag ggtcggcgca ccgcccgcac gggaaatgat cgactgttca cccacttcgc
     6361 tggcaatctg gtttagcgta tccggggtaa tcgcaaagcc ccagctactc accacctgcg
     6421 cggcagaagc aaccacatcc gccgtccctg ccggagccag cacagccatc gggctgttca
     6481 tggcgaagta cacacccgga tcgatgatac aggcggaaac cagcgccata atcgccacga
     6541 aggattccat taacatccca ccgtaaccga taaagcacgc ctgcccttcg ttcgccagca
     6601 tcttcggtgt agtgccggaa gagatcagtg catggaagcc agacaccgcg ccacaggcga
     6661 tggtgataaa caggaacggg aacaggttac cggtccatac cgggccagtg ccatcgacaa
     6721 atttagttag cgcaggcatg gtcagcgtcg ggcgcataat caaaatgccc accgccagac
     6781 cgacgatagt cccgattttc aggaaggttg agaggtagtc acgcggagcc agcagcaacc
     6841 acaccggcag caccgccgcc acaaaaccgt aacccaccag catccaggtc agctgcacgc
     6901 cggtaaagtc aaagtacggt gcccaggtcg gactttctgc cacccagccg ccagagataa
     6961 tggcgaaaat caggaacacc aggccgatga ccgacacttc gccaatacgc cccggacgca
     7021 gatagcgcag gtagatcccc ataaacagcg ccagcggaat ggtgaacgca acggtatacg
     7081 ttccccacgg gctatgagtc agggctttaa ccacgatcat cgccagcacg gcaaggataa
     7141 tgaccatgat cataaagcag gccaccagcg ctatcacccc ggcggttggc cccatctctt
     7201 ctttgaccag ctcacccagc gagcgaccgt cacggcgcgt agaaacaaac agcaccatga
     7261 aatcctgcac cgcaccggcg agaaccaccc cggcgagcag ccagatcatc cctggcaggt
     7321 aacccatttg cgccgccagt accggcccaa ccaacggacc tgctccggca atggccgcaa
     7381 aatggtgacc gaacagcact tttttgtccg tcggcacata gtccagcccg tcgttatgac
     7441 gcaccgctgg cgtcatacgc gtcgggtcaa ccgccagcac atttttggcg atatacagcc
     7501 cataaaaacg gtaagcgatc agatagatac agaccgacgc caccacaatc cacagcgcgt
     7561 tgatctgttc cccacgattt aaagcaatgt atcccagagc aaatgctccc attacagaga
     7621 gcactgtcca gacgaggtat ttccctgatt tgttcatagt tgttatccgt gtgcgtgtcc
     7681 atagagatgt tacattttgt ttctataaca tctttgtaca atttaacaac acagcaacca
     7741 tgtaaatcgg agttgaaacc aatatttaac ttaacaatgt taactctatc actccgttaa
     7801 ccgaattttc ctgcaatctc attttattct gtcgggttca tcccaaaacc gctgtgccca
     7861 gccgacaggt gcagcaacgc cgcccctgtt catcgaaaac gacgatttcc cagctctgat
     7921 tttgtcgtcc aagatgcagc ggctggcaga caccgcgcac ctttccctca gacaccgggc
     7981 gatgatgtgt ggcgttaagt tccgtgccca ccacacactg tccgtcgcgg gtcatcataa
     8041 atccggccat cgatcccagc gtttccgcca gcgccgccga cgcgccgcca tgcagcaggc
     8101 caaacggctg atgagtacgg gtatcaaccg gcatttcggc ttcgagtaca tcatcgccca
     8161 gacgggtata cacaattccc agatgcgcca ccattgtgtt atcgctggtg gcgttcagct
     8221 cgtcgagcgt taaatggcgt ttccagatca tgcttatgcc cccagcgttg agccgccatc
     8281 gaccacaata tcctgtaggg taatatggct ggcgaggtca gaggcgagga acaaaatcgt
     8341 gttggcgatc tcctgcggac gggcgatttt ccccagcgga atgccgagtt taaactgctc
     8401 gccaaagccg cgaatacgct gctcttcggc gtcatcgctc acccacagcg tgcgttgcat
     8461 atcagtgtcg gtggagcccg gcgaaaccac attacagcgc acgccgctac ccgccagttc
     8521 cagcccgacg ctcaacgcca ggcttttcag cgccgctttc gacgcgccat aagcactcat
     8581 accaatacgc ggcgtgtgtg cggcgtcgga cgcaacagta acaatcgccc cgccccgctg
     8641 acggcgaaac tggttcatgg tctgctgaaa caggttaaat gcaccgccga cgttgaccgc
     8701 aaaagtctgc tgccagtcct ctttactgag ctgatcggtc gcgcccatgc gcaaaattcc
     8761 cgccgcattg accagcacgt ccagtcgctc cgtttcagct aacagtcgct gacatacctg
     8821 cgccacctgc gcagcatcgg caacatccat tacttcggtc gcaaaaggat attgctcctg
     8881 agtaaacgct tgatcaaaac ctgtaacttt cgcccccgcc tcaacaaatg ccagcgccgt
     8941 ggcatagccg atacctttcc ccgcgccagt tacccagaca tttttaccgc tgaaatccat
     9001 tatttcacct cgcgggagag tagcttccac caggcgtcga tggtcgggtt tttcgctagc
     9061 atgacaaagt cgatatcgcc atgcactttg cgccagcgcg ccgccagcgc catcatgcgc
     9121 accgaatcca gaccgtagtc gatcaggttg tcgtcatcga agggttcatc ggactcgtcc
     9181 agcaacggca ggatcacctc acgcagctcc gctttgctgg cggggatagg tgctggcagc
     9241 aattcttcgg tcatcaccac ccgaccagaa cgtccggcca catatttcag cgacatcaaa
     9301 tgctcgtcac ggctgaaatc ggccagcgca tccgccacca taaacggttt aatatcgcgc
     9361 ataaatgcgt ccgttgcggt ggtcatacag ccaatgtggg catatacccc ggtaataatc
     9421 agctggttac gtccactctc tttgagcatt tgctccagcg gagaacgatg aaatgcgctg
     9481 tagcgccatt tcaccagcac cgtgtcgtcg gcatctggcg tcaggcgatc caccaccttt
     9541 tgctgttccg gcgaacgggt caggccaggc ccccacatat cattcaacag cgcccgatct
     9601 tcatcgctct gctctttcgg ctgggcggtg taataaaccg ggatattgtg ctgtttgcag
     9661 tagtcgcgta gtgccgcaat attcgcgatc acctgctcca tcatcgggca gttctcaccc
     9721 cagaagctga caaaatagtc ctgcatatca tggattaaca acgcggcacg ttgcggttca
     9781 aaggcccagt cgactttatt ctgcggaata tcgtgagact ccggcagtgc gtaagcctgt
     9841 aattttggaa tagccatcgt gttctctcct tcaggctgat gtgcgtgacg ccagccactg
     9901 acgtaattgt tttttatcga ctttcccgac cgccgtcagc ggaagtgaat ctacacactc
     9961 cacgcgatcc ggtaatttaa attcggcaat accctgttca cgcaggaaac gacgcacctg
    10021 caccgcgcgc agcggctctt ttaccaccag ataagcacag cttttttcgc ccatcagctc
    10081 atcttccatg ctcaccagtg cggcgtagat caccgccgga tggcgcagca gcaggttttc
    10141 gatctcttcg gcagcgatct tctcgccgcc acggttgatc tgatctttct cgcgcccctg
    10201 cacagtgatg taaccctctg gatcaataga gatcagatcg ccggaacagt aaaaaccgtt
    10261 ggcatcaaag gcgctggcat tgtgctgcgg gcttttgtaa tagccacgga aggtgtacgg
    10321 cccgcgcgtc atcaggcgtc caacttcccc ttgcggcagt ggatttcctt cggcatcggc
    10381 aacccatact tcatcgtccg ggcacattgg gtaaccctgg gtatggataa ttttctccgc
    10441 gctatcatca agtcgggtgt agttcaccag cccttccgcc atgccaaaca cctgctgcaa
    10501 ctggcagcca atctcagcgg gaatacgcgc cgcaagcgtg gcagaaagac gtgcgccgcc
    10561 gacctgcaac agtttcagcg aggcaagctg cgcccggctt tcgccttcag ccagtgtctg
    10621 caaccacagg ctgactgctg gcggcaccag cgcggtgacg ttcacctgat gtttttcaat
    10681 caatgggaag caaagcgtgg cgctggggtc ggcagccaga acgacagtgc cgccagcgag
    10741 aaataccccc aacgatcccg gtgaactcat ggcgtagtta tgagccgccg ggatcgcgca
    10801 caggtagcgc gtctgttgtg tgaactgaca aatctcgacg ctacgacgca cgctgtagta
    10861 gtagtcgtta tgagtgcgcg ggatcagttt cggagtgccg gtggtgccgc cggaaagctg
    10921 gaaataggcc acttcatcag caggagatgg cgtggcagta aaatcctcag ccggatggtt
    10981 aatcgcatcc tgcaagttat gctcaccgct gtcgttgagc agctgcacca tgcgaatcga
    11041 agaatgctct gcaacaaatg tgttgaggaa atcatccccg ctaaatagcg catgttggcg
    11101 atcggcaatc agtaacgccg gttcaatctg gctggcatag gcgttcagtt cactacgctg
    11161 atggctgaac agcgccagca ccggcgcaac gcccagtttc agcagcgcga aaaaggtaat
    11221 gtaaagttca gcgacgttac ccagttgtac cagcgcggtt tcaccaggtt taatgccctg
    11281 acggcgcaaa ctacaggcga ggttatccgc cgcctgattc agctcccggt aactcaactg
    11341 ccgctcgccg tcgataaccg cgatgctgtc actcgcaacg tggcgagtca gaatgtcggt
    11401 cagtggcaaa tcctgccagt agcctttttc ccgatagcga cgggcaaact cttccggcca
    11461 gcgggtgaat ggaatgctca tcctcgctcc ttaatgcaat ccaaaaacgt tcaacatggt
    11521 agaaagtttg acgcccgttt cgcgccactc acccaacggt gaggacgcag gcacaatccc
    11581 cgctccggca aacagacgca cctgattttc ccgcagcttc gcgcagcgga tggtcaccac
    11641 ccattcaccg ttaccttcgc tgtcacacca acccacaatg ccgccaaaca gttcgcggtc
    11701 gaacggctcc agttcagcaa taacctgagt cgcggcctga tgcgggaaac cgctcagcgc
    11761 gggggtcgga tgcagcagac aagccaaagt cagtgcgttt tcttgcgaat tcgctttacc
    11821 ttcaaagggc gttgcgagat gccacagcgt cggcgtggta atcaattgtg gagaggaggg
    11881 aacgtgtaac tcactactgc gtttgcgcag tacctctttc atcgcctgag tcaccagttc
    11941 atgttcatgg cgatcttttt ctgacgccag cagacgatta cccgcttcgc gatcgagcac
    12001 ttcatccggc tgacgacgcg cggaaccggc taacggaatg gagctaaaac gctcgccgtc
    12061 tttacgtagc agcagttccg ggctggcccc cagcagaacg ccaccatcag ccaacggaac
    12121 atggaagttg taactaaccg ggttttgcgc aatcaaccgt tccagcaata cgccactatc
    12181 aatggcggcg tcagtggtga tatcaatcaa tcgtgacaac accactttgt cgacctgcgg
    12241 cgtggcggta agtgcggcag cgcgggcaac catctgttca aacgtggttt gttccggaat
    12301 ggcctgacgt tccaccacgt tcagcgactg gctgcgagtg aaacggcgtg cggaagcttg
    12361 tttttcctga cgggagaacg actgccagga ttcaggtata tacagcgacg aaggctgacg
    12421 tggatcgaag ggaatcgccc cgaccatcac cggatttttg atgccctgcg ctttggcatc
    12481 ggcaaacaac gcggcgagtt tttgctggaa gggactgtcg ggcgaatccc cgttcacagc
    12541 cggttcatcg aagcgggcga aacatcctga cgtcgtaaaa ctgcggtacg gcgacataaa
    12601 gaaaaagcga ttgggcgcaa gtgttgccat ggtctgctgt acttcctcag ccagtgacgt
    12661 atccatatca tcctccacaa aatgataaag gctttaataa tgattatcat ttatattttc
    12721 ggtcgctaac ctaaaagcaa acgcgcacta tgtcaactct tgaggtaacg caatctgcaa
    12781 ctcggggttg catctggttg tggttacaat gaaaatgaga agcattaact tcattaataa
    12841 caggaagctg acttgtgaga ctcgccccgc tctaccgcaa cgcccttcta ttaacaggac
    12901 ttttgctttc aggaatagcc gcagttcagg ccgccgactg gccgcgtcag attactgaca
    12961 gccgtggcac tcataccctg gaaagccagc cgcagcgtat agtttccacc agtgtcaccc
    13021 tgaccggctc actgctggcg attgatgctc cggtgatcgc cagcggcgcg accacgccaa
    13081 ataaccgcgt cgcggatgac cagggctttt tacgccagtg gagcaaggtg gcgaaagaac
    13141 gcaaactgca acggctctat atcggcgaac cgagtgcaga agccgttgcc gcgcaaatgc
    13201 cggatctgat tttaattagc gcaaccggcg gcgattcggc gctggcgctg tacgatcagc
    13261 tttctaccat cgccccaaca ttaatcatca attacgacga caaaagctgg cagtcgctgt
    13321 taacgcagct tggcgaaatt accgggcatg agaaacaagc ggcagagcgg attgcacagt
    13381 ttgataagca actggcggcg gcgaaagagc aaatcaaatt accgccgcag ccggtcactg
    13441 ccatcgtcta taccgccgct gcacacagtg ccaatctctg gacgccggaa tcagcacaag
    13501 ggcagatgct ggaacaactc ggctttacgc tggcgaagtt gcccgcaggg ttaaacgcca
    13561 gccaaagcca gggcaaacgc catgacatca ttcagcttgg tggagagaat ctggctgcag
    13621 ggttaaatgg cgagtcacta ttcctgttcg ccggtgatca gaaagacgcc aatgctattt
    13681 atgctaatcc gctgctcgcg cacctgcctg cagtacaaaa caagcaggtt tatgcgctgg
    13741 gaaccgagac attccgcctg gattactaca gcgccatgca agtgctggaa cgacttaagg
    13801 cgctgtttta agcattaact gtcggacgct gtcacctgcg gcggcgtctg gcgaaaacgc
    13861 cgcaactcca ccagcaccag cagcaacaac acgccgataa tcaacaaacc aaaaccgctc
    13921 gcacttgcgg aagcaaccgg tgtcatcatc gctcccaaac cgcccagcag cgccgcgccc
    13981 atggcatcgc ccgtcacgtt ttgcgccgtc cacaaaccgt taatccgccc taacatcgct
    14041 tccggggttt gcgtttgcag cattgtatat tgcagcaacg aactaaccgc actcagccag
    14101 ccgaacagtg ccagacaaac cacgcctaaa atccacatcg gcatcaggcc aaacagacca
    14161 atggcgagga acgcccccaa tgtggagagc aacatcaaca gacctggccg tacgctatgc
    14221 gccagttttc cactggttaa cgcgccaata gccgcaccga gcgggattgc cgcatagaga
    14281 aagccaattt gcgccgccga catctgccag ttgtcagcca gcgccggata caggacccgc
    14341 accgcgctcg ccatcgtcaa taaccccccc agcaacgcaa tccctcccac cagcgggctg
    14401 gcgagtagaa aacgaaatcc tgccagtaat gatttcaacg gatgctcacg cggctgcggc
    14461 ggtggtggca acgccggaag gcttaacaac ggtagcaagg taataaacgt gcccgccgcc
    14521 gccagcccgt agttccaggc tacgcctccg gtcgccagca gtaaaccgcc aatcatgggc
    14581 gaaatcaccg atcccagacg cacggtcaac atggtgatcg ccccggcctg cattaggttt
    14641 tcacgcccta ccagtgcagg tgtcgccgcc agtagcgccg taacgccaag tgatgcgaaa
    14701 aaaccatccc ataaaccaag taaatagatt gccagcaaag acggctccgg cagcagcgca
    14761 ttcaggcaca gtccaatgaa gccaatgcca caggtgccgc gcgccagcaa aatcactttt
    14821 ttacgctcat agcgatccgc cagcacaccg ccgaccatca ggccaacaaa catcgcgccg
    14881 ccggtcagcg tcaccgaaag ccccacctgc caggtagaat gcgtcatcat ctggatctgc
    14941 accggcaccg cgacgccgag caaacccaga gaaacaattg agataaaacg agcgaggaat
    15001 actgcgcgaa acgccgggtg cgttttcaac aggctgaggt taagcagcca ggattgttta
    15061 ttcattacaa tgccttgcca tcaaatattt taaatatcta ttcttaggct gcacatgcta
    15121 acatatccaa ataagatcga taacgataat taatttcatt atcatggaag ttcgtatgtc
    15181 tggttctgtt gccgtgacac gcgccattgc cgtgcccgga ttgctgttat tactgattat
    15241 cgcgacggca ttaagcctac tcattggggc aaaatcactc cccgcttccg tagtgctgga
    15301 ggcgctctcc ggcacctgcc agagcgccga ctgcaccatt gtgctcgacg cccgtctgcc
    15361 gcgcaccctt gccggtttac tggcaggcgg tgcgcttggc cttgccgggg cgttaatgca
    15421 aaccctcacc cgaaacccgc ttgccgatcc tggtttgctt ggcgtgaacg ccggtgccag
    15481 ttttgccatt gtgctgggcg cggcgctgtt tggttactct tccgcgcagg aacaactggc
    15541 gatggccttc gccggcgcgc tggtggcctc gttgattgtt gcctttaccg gcagccaggg
    15601 cggcgggcag ttaagtccgg tgcgtttaac cctggcgggc gtggcgctgg cggcggtgct
    15661 ggaagggctg accagcggca tcgccctgct taaccctgac gtctacgatc agttgcgttt
    15721 ctggcaagcc ggttcactgg atattcgcaa tctacatacc ttaaaagtgg tgctgatccc
    15781 ggtgcttacc gctggagcaa ccgcgctatt actgagccgc gcgctgaaca gtttaagcct
    15841 tggcagtgac accgcgacgg cgctgggcag tcgcgtagcg cgcacacagt tgattggtct
    15901 gctggcaatt accgtgcttt gtggtagtgc gacggcggta gttggcccga ttgcctttat
    15961 tggcctgatg atgccgcaca tggcgcgctg gctggtaggt gccgatcatc gctggtcgct
    16021 gcccgtcacg ctactcgcta cccctgccct gctgctgttt gccgatatca ttgggcgggt
    16081 gattgttccc ggcgaactgc gcgtttctgt agtcagtgcc tttattggtg caccagtgct
    16141 gatcttcctc gtacgacgta aaacgcgagg tggcgcatga tttacgtctc tcgccgatta
    16201 attatcacct gtctactgtt gctaatagct tgtgtgatgg caggcgtgtg gggattacgt
    16261 agcggtgccg tcacgctgga aacttcgcag gtattcgccg cgttgatggg cgacacgccg
    16321 cgcagtatga cgatggtggt caccgaatgg cgtttaccac gcgtgctgat ggcgctgttg
    16381 attggcgcgg cgctgggcgt cagcggcgcg atttttcagt cgctgatgcg taacccgctc
    16441 ggcagccctg acgtaatggg ctttaacacc ggggcatgga gcggcgtact ggtggcgatg
    16501 gtgctgtttg gtcaggatct gacggctatc gcgctggcag caatggcggg gggtattgtc
    16561 acctcgctgt tggtctggct gctcgcctgg cgtaacggta tcgacacctt tcggttgatt
    16621 attatcggta tcggtgttcg cgccatgctg gtggccttta atacctggct gttgctgaaa
    16681 gcgtctttag aaacggcgct aacagcgggt ttgtggaatg ccggatcgct caacggcctg
    16741 acgtgggcaa aaacctcacc ttccgcgcca atcattatat tgatgctcat tgccaccgcc
    16801 ttgctggtgc ggcgaatgcg cttgctggaa atgggcgatg ataccgcatg tgcgctgggc
    16861 gtcagcgtcg aacgttcgcg gctgttaatg atgctggtcg ccgtagtgct taccgcagct
    16921 gccactgccc tggcggggcc gatttccttt attgctttag tcgcgccaca cattgcccgc
    16981 cgcattagcg gcaccgctcg ctgggggctg acccaggcgg cgctgtgcgg ggcgttgtta
    17041 ttactggcgg ccgatctctg cgcccaacaa ctgtttatgc cgtatcaact tccggttggc
    17101 gtcgttaccg tcagcctcgg cggtatttac cttatcgtct tgttaattca ggagtctcgc
    17161 aaaaaatgac cgaatcagta gcccgtttgc gcggcgaaca gttaaccctc ggatatggca
    17221 aatataccgt tgcggagaat ctgactgtag aaatacctga tggtcacttc acggtaatta
    17281 tcgggccaaa tggctgcggt aaatccactt tactgcgtac tttaagccgc ctgatgacgc
    17341 ctgctcacgg gcatgtctgg ctggatggtg agcacattca acattacgcc agtaaagagg
    17401 ttgcacgccg gattggtctg ttggcgcaaa atgctaccac gccgggcgat atcaccgtgc
    17461 aggagctggt ggcgcgtgga cgttacccgc atcaaccgct gtttacccgc tggcggaaag
    17521 aagatgaaga ggcagtcaca aaagcgatgc aggccacggg aatcacccat ctggcagatc
    17581 aaagcgtgga taccctttct ggcggacaac gccagcgagc gtggatcgcg atggtgctgg
    17641 cccaggaaac ggcaattatg ctgctcgatg aacccacgac ctggctggat atcagtcatc
    17701 agattgattt gctggaactg ttaagcgaac tgaaccgcga gaaaggctat accctggcgg
    17761 cggtgctgca cgatcttaat caggcctgtc gttacgccag ccatttgatt gcattgcggg
    17821 aagggaaaat tgttgctcag ggagcaccga aggagattgt cactgctgaa ctgatcgagc
    17881 gcatttatgg tctgcgctgc atgattattg acgatccggt tgccggaacg ccgctcgtgg
    17941 tgccgctcgg acgaacggca ccttcaaccg caaagattta aactaagtgg tctgccatca
    18001 tggcatcctg ttttctggat gccatcgcat gacgcaacag cacaccacca caagccacca
    18061 tcccgccgat caacgcggaa aggatcacaa taatagcctt acccggaccg tcttttttca
    18121 ctggcaatga cggtcttaac tgatatttaa acggcgtaaa attcacatcg ttgatatttg
    18181 cttttgttaa ctgctcaaca agatactggc gattacgtaa ttcgccgttc agttccgcaa
    18241 cgtcagtaac cgctttttca atttccagtt tgcgttcaat accgtctgcg ccgagagaaa
    18301 tagagaaatc ggggtcatct ttaacggcct gaccattact gtatacgggc tttttaattc
    18361 ctgccgcgtt ggcaatgtcg agtgaataat tgaggcgctg aatgtttgca tcaagttgat
    18421 ttttcatttt aatgcggtcc tgagccagtt ttgctttttc aaactgggtt ttgatctcca
    18481 gtttattacg gacgttttct atcgactctt tcaccaccaa cacagagata taatcgatat
    18541 acccgctcaa aacggtctgc gcctcttcac tggttggcgc ggtaaaactt agcgtccagg
    18601 aggtatacag tgacggttca tcttttttct tactggcatt gtcatcaacc gctttcattt
    18661 tttcgctcaa tgcgacaatt gcgcgatgca aatccagttc gtcgattttc gcctctttta
    18721 attggtccat tacataaggt gatgagcgca ggtactcttc tagtaagcta acagactgaa
    18781 acttcttgat aaacaggtta aatgcaccgc cacggtcgat attaacatcg agatcaagaa
    18841 cgcggagttt ggtaaaggtt ttctccaggt cctgccactg aatggcttcg gcgggagtaa
    18901 tgacggctga gctggtccat ttttgcggca ggatgaaaga gatcagcaag cctgcgcagg
    18961 caaacgcaaa aacgaccgcc atgaccgttt ttttggcccg ccataaaact tcaattagac
    19021 taagtaaatc aatttcatta ttactgggcg acgccagagg atactcggga aaatgagctt
    19081 cacttccctg tttaatattc agtgatgaca tggggtataa cctgaaaatg agtccaataa
    19141 gagtaaatct ccacggcccc cactctacca gttggtgaga tttatccgaa gctgaagttt
    19201 gtaaattact cccactgaat gcaacattca ctctatttca gagaaata
//
LOCUS       DADSYM010000093        19214 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2415
            SAMN08039579-rid11723173.denovo.093, whole genome shotgun sequence.
ACCESSION   DADSYM010000093 DADSYM010000000
VERSION     DADSYM010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039579
            Sequence Read Archive: SRR6362533
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 19214)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19214)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980162.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 03:39:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,484
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 4,347
            CDSs (with protein)               :: 4,347
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 43 of 85
            Pseudo Genes (incomplete)         :: 37 of 85
            Pseudo Genes (internal stop)      :: 19 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19214
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039579-rid11723173.denovo.093"
                     /strain="LGE2415"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8302 N 95.9628 W"
                     /collection_date="2015-08-24"
     gene            <1..1349
                     /locus_tag="J1Y83_002814"
     CDS             <1..1349
                     /locus_tag="J1Y83_002814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001538823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sel1 repeat family protein"
                     /protein_id="HBA7630947.1"
                     /translation="ITPFFASNLSPLEEMENGKEMATIYQSLKDNEESLKWFDIAVLN
                     KVAEFELSDNYMALLIECLSYSMRKEKMIRKWKLTERFNNLAMQHHSGYKKLLNAIKL
                     KGLFYYPSSVYQSCLHAAEQGYSLAWYSLGKMYYEGDEVSQDLKLAFNWFTRAAQHNV
                     IDALYALGIMYSDGRGTDKNICEARKWFLLAAQNGNTSAQYELARISRFAVEPLRNYE
                     EALQWYLSAATQGHEVAQYELGQMYIQGIGVERDEVQAHRWFLQSAEQGYLHAQYHTA
                     RLYSGSESIPQDQEKALYWFTKAAKNGADGAGDAMYELGKYYLTNNDDPENNAEATQW
                     LTGAAQRGRIEAIFLLAEMYLYGTKYIAKDENHALHWYEKAARLGATEAQHQTAAMYA
                     QGTGTKIDNKQAWMWLTIAGNNNPWIEKDDLRCLMSEQDIQDAEQTAANCKRLLRI"
     gene            complement(1405..2748)
                     /gene="gdhA"
                     /locus_tag="J1Y83_002815"
     CDS             complement(1405..2748)
                     /gene="gdhA"
                     /locus_tag="J1Y83_002815"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013366584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HBA7630948.1"
                     /translation="MDQTYSLESFLNHVQKRDPNQTEFAQAVREVMTTLWPFLEQNPK
                     YRQMSLLERLVEPERVIQFRVVWVDDRNQVQVNRAWRVQFSSAIGPYKGGMRFHPSVN
                     LSILKFLGFEQTFKNALTTLPMGGGKGGSDFDPKGKSEGEVMRFCQALMTELYRHLGA
                     DTDVPAGDIGVGGREVGFMAGMMKKLSNNTACVFTGKGLSFGGSLIRPEATGYGLVYF
                     TEAMLKRHGMGFEGMRVSVSGSGNVAQYAIEKAMEFGARVITASDSSGTVVDESGFTK
                     EKLARLIEIKASRDGRVADYAKEFGLVYLEGQQPWSVPVDIALPCATQNELDVDAAHQ
                     LIANGVKAVAEGANMPTTIEATELFQQTGVLFAPGKAANAGGVATSGLEMAQNAARLG
                     WKAEKVDARLHHIMLDIHHACVEHGGEGEQTNYVQGANIAGFVKVADAMLSQGVI"
     gene            2984..3256
                     /locus_tag="J1Y83_002816"
     CDS             2984..3256
                     /locus_tag="J1Y83_002816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnjH family protein"
                     /protein_id="HBA7630949.1"
                     /translation="MSRALIAVVLAFPLITLANPHYHPDVEVNVPPEVFSSGGQSAQP
                     CTQCCVYQDQNYSEGAVIKAEGILLQCQRDDKNLSTNPLVWRRVKP"
     gene            complement(3222..3629)
                     /gene="nudG"
                     /locus_tag="J1Y83_002817"
     CDS             complement(3222..3629)
                     /gene="nudG"
                     /locus_tag="J1Y83_002817"
                     /EC_number="3.6.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP pyrophosphohydrolase"
                     /protein_id="HBA7630950.1"
                     /translation="MKMIEVVAAIIERDGKILLAQRPAQSDQAGLWEFAGGKVEPDES
                     QRQALVRELNEELGIEATVGDYVASHQREVSGRIIHLHAWHVPDFHGTLQAHEHQALV
                     WCSPEEALQYPLAPADIPLLEAFMALRAARPAD"
     gene            3716..4336
                     /locus_tag="J1Y83_002818"
     CDS             3716..4336
                     /locus_tag="J1Y83_002818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707357.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HBA7630951.1"
                     /translation="MLDRHLHPRIKPLLHQCVRVLDKPGITPDGLTLVGFAIGVLALP
                     FLALGWYLAALVAILLNRLLDGLDGALARRRGLTDAGGFLDISLDFLFYALVPFGFIL
                     AAPEQNALAGGWLLFAFIGTGSSFLAFAALAAKHQIDNPGYAHKSFYYLGGLTEGSET
                     ILLFVLSCLFPACFPWFAWVFGALCWMTTFTRVWSGYLTLKSLQRQ"
     gene            complement(4345..5652)
                     /locus_tag="J1Y83_002819"
     CDS             complement(4345..5652)
                     /locus_tag="J1Y83_002819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310490.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase"
                     /protein_id="HBA7630952.1"
                     /translation="MKRVSQMTALAMALGLACASSWAAELAKPLTLDQLQQQNGKAID
                     TRPSAFYNGWPQTLNGPSGHEPAALNLSASWLDKMSTEQLNEWIKQHNLKADAPVALY
                     GNDKDVDAVKTRLQKAGFTHISILSDALSEPSRLQKLPHFEQLVYPQWLHDLQQGKEV
                     TAKPAGDWKVIEAAWGAPKFYLISHIPGADYIDTNEVESEPLWNKVSDEQLKAMLAKH
                     GIRHDTTVILYGRDVYAAARVAQIMLYAGVKDVRLLDGGWQTWSDAGLPVERGTPPKV
                     KAEPDFGVKIPAQPQLMLDMEQARGLLHRQDASLVSIRSWPEFIGTTSGYSYIKPKGE
                     IAGARWGHAGSDSTHMEDFHNPDGTMRSADDITAMWKAWNIKPDQQVSFYCGTGWRAS
                     ETFMYARAMGWNNVSVYDGGWYEWSSDPKNPVATGERGPDSSK"
     gene            complement(5719..6372)
                     /locus_tag="J1Y83_002820"
     CDS             complement(5719..6372)
                     /locus_tag="J1Y83_002820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707358.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBA7630953.1"
                     /translation="MLCVKNVSLRLPESRLLTNVNFTVDKGDIVTLMGPSGCGKSTLF
                     SWMIGALAGQFSCTGELWLNEQRIDMLPTAQRQIGILFQDALLFDQFSVGQNLLLALP
                     ATLKGNARRNAVNDALERSGLDGAFHQDPATLSGGQRARVALLRALLAQPQALLLDEP
                     FSRLDVALRDNFRHWVFSEIRAMAIPVVQVTHDLQDVPPDSPVLDMAQWSENYNKLR"
     gene            complement(6372..7907)
                     /locus_tag="J1Y83_002821"
     CDS             complement(6372..7907)
                     /locus_tag="J1Y83_002821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBA7630954.1"
                     /translation="MATPLRYALIFLLWAMMAVIYAPLIPAALTLISPALSLTHWQAL
                     FADPQLPQALLATLESTTIAAVGALLIALLVIVALWPGPKWQRMCARLPWLLAIPHVA
                     FATSALLLFADGGLLYDYFPYFTPPMDKLGIGLGFTLAVKESAFLLWILAAVLSEKRL
                     LQQLIVLDSLGYSRWQCLNWLLLPSVAPALAMAMLAIVAWSLSVVDVAIILGPGNPPT
                     LAVISWQWLTQGDADQQTKGALASLLLMLLLAAYVLLGYLLWRGWRRTIPRVDGVRKP
                     ATPLLPGISLASFLPLTGVLCVVLLAILADQSTINSEALINSLTMGLVATFIALLLLL
                     LWLEWGLQRRQLWLWLPILLPALPLVAGQYTLALWLNQDGSWTAVVWGHLLWVMPWML
                     FILQPAWQRIDSRLILIAQTLGWSRSKIFFYVKCPLMLRPTLIAFAVGFSVSIAQYMP
                     TLWLGAGRFPTLTTEAVALSSGGSNGILAAQALWQLLLPLIIFALTALVAKWVGYVRQ
                     GLR"
     gene            complement(7880..9046)
                     /locus_tag="J1Y83_002822"
     CDS             complement(7880..9046)
                     /locus_tag="J1Y83_002822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBA7630955.1"
                     /translation="MRHCGWLLGLLSLFSLATHASDWQEIKNEAKGQTVWFNAWGGDT
                     AINRYLDWVSGEMKTHYAINLKIVRLADAADAVKRIQTEAAAGRKTGGSVDLLWVNGE
                     NFRTLKEANLLQTGWAETLPNWRYVDTRLPVREDFSVPTQGAESPWGGAQLTFIARRD
                     VTPQPPQTPQALLEFAKANPGTVTYPRPPDFTGTAFLEQLLIMLTPDPAALKEAPDDA
                     TFARVTAPLWQYLDALHPYLWREGKDFPPSPARMDALLKADTLRLSLTFNPAHAQQKI
                     ASGDLPASSYSFGFREGMIGNVHFVTIPANANASAAAKVVANFLLSPDAQLRKADPAV
                     WGDPSVLDPQKLPDGQRETLQSRMPQDLPPVLAEPHAGWVNALEQEWLHRYGTH"
     gene            complement(9056..9604)
                     /locus_tag="J1Y83_002823"
     CDS             complement(9056..9604)
                     /locus_tag="J1Y83_002823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HBA7630956.1"
                     /translation="MGLPPLSKIPFILRPQAWLHRRHYGEVLSPIRWWGRIPFIFYLV
                     SMFVGWLERKRSPLDPVVRSLVSARIAQMCLCEFCVDITSMKVAERTGSTDKLLAVAD
                     WRQSPLFSDEERLALEYAEAASVTPPTVDDALRARLATHFDAQALTELTALIGLQNLS
                     ARFNSAMDIPAQGLCRIPEKRS"
     gene            complement(9604..10311)
                     /locus_tag="J1Y83_002824"
     CDS             complement(9604..10311)
                     /locus_tag="J1Y83_002824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HBA7630957.1"
                     /translation="MMKMQSRKIWYYRITLIILLFAVLLAWALLPSVHEFINRSVAAF
                     AAVDQQGIERFIQSYGALAAVVSFLLMILQAIAAPLPAFLITFANASLFGAFWGGLLS
                     WTSSMAGAALCFFIARVMGREVVEKLTGKTVLDSMDGFFTRYGKHTILVCRLLPFVPF
                     DPISYAAGLTSIRFRSFFIATGLGQLPATIVYSWAGSMLTGGTFWFVTGLFILFALTV
                     VIFMAKKIWLERQKRNA"
     gene            complement(10325..11002)
                     /gene="ydjY"
                     /locus_tag="J1Y83_002825"
     CDS             complement(10325..11002)
                     /gene="ydjY"
                     /locus_tag="J1Y83_002825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YdjY"
                     /protein_id="HBA7630958.1"
                     /translation="MLQHYSVSWKKGLAALCLLAVAGLSGCDQKENAAAKVEYDGLSN
                     SQPLRVDANNHTVTMLVQINGRFLTDDTRHGIVFKDGSNGHKSLFMGYATPKAFYEAL
                     KEAGGTPGENMTMDNKETTHVTGSKLDISVNWQGAAKAYSFDEVIVDSNGKKLDMRFG
                     GNLTAAEEKKTGCLVCLDSCPVGIVSNATYTYGAVEKRGEVKFKGNASVLPADNTLAT
                     VTFKITE"
     gene            complement(11007..11717)
                     /locus_tag="J1Y83_002826"
     CDS             complement(11007..11717)
                     /locus_tag="J1Y83_002826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HBA7630959.1"
                     /translation="MNAERKFLLACLIFALVIYAIHAFGLFDLLTDLPHLQTLIRQSG
                     LFGYSLYILLFIIATLFLLPGSILVIAGGIVFGPLLGTLLSLIAATLASSCSFLLARW
                     LGRDLLLKYVGHSHTFQAIEKGIARNGIDFLILTRLIPLFPYNIQNYAYGLTTIAFWP
                     YTLISALTTLPGIVIYTVMASDLANEGITLRFILQLCLAGLALFILVQLAKLYARHKH
                     VDLSASRRSPLSHPKNEG"
     gene            complement(11884..12690)
                     /gene="xthA"
                     /locus_tag="J1Y83_002827"
     CDS             complement(11884..12690)
                     /gene="xthA"
                     /locus_tag="J1Y83_002827"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HBA7630960.1"
                     /translation="MKFVSFNINGLRARPHQLEAIVEKHQPDVIGLQETKVHDDMFPL
                     EEVAKLGYNVFYHGQKGHYGVALLTKETPIAVRRGFPGDDEEAQRRIIMAEIPSPLGN
                     VTVINGYFPQGESRDHPIKFPAKAQFYQNLQNYLETELKRENPVLIMGDMNISPGDLD
                     IGIGEENRKRWLRTGKCSFLPEEREWMERLMSWGLIDTFRHANPQTADRFSWFDYRSK
                     GFDDNRGLRIDLLLASQPLAECCVETGIDYEIRSMEKPSDHAPVWATFRR"
     gene            13136..14356
                     /gene="astC"
                     /locus_tag="J1Y83_002828"
     CDS             13136..14356
                     /gene="astC"
                     /locus_tag="J1Y83_002828"
                     /EC_number="2.6.1.11"
                     /EC_number="2.6.1.81"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinylornithine/acetylornithine transaminase"
                     /protein_id="HBA7630961.1"
                     /translation="MSQPITRENFDEWMIPVYAPAPFIPVRGEGSRLWDQQGKEYIDF
                     AGGIAVNALGHAHPELREALNEQASKFWHTGNGYTNEPVLRLAKKLIDATFADRVFFC
                     NSGAEANEAALKLARKFAHDRYGSHKSGIVAFKNAFHGRTLFTVSAGGQPAYSQDFTP
                     LPPDIRHAAYNDINSASALIDDATCAVIVEPIQGEGGVVPASNAFLQGLRELCDRHNA
                     LLIFDEVQTGVGRTGELYAYMHYGVTPDLLTTAKALGGGFPVGALLATEECASVMTVG
                     THGTTYGGNPLASAVAGKVLDLINTPEMLNGVKQRHDWFVERLNSINHHYSLFSEVRG
                     LGLLIGCVLNADYAGQAKQISQEAVKAGVMVLIAGGNVVRFAPALNVSEEEVTTGLDR
                     FASACEHFVSRGSS"
     gene            14353..15387
                     /gene="astA"
                     /locus_tag="J1Y83_002829"
     CDS             14353..15387
                     /gene="astA"
                     /locus_tag="J1Y83_002829"
                     /EC_number="2.3.1.109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine N-succinyltransferase"
                     /protein_id="HBA7630962.1"
                     /translation="MMVIRPVERSDVSALMQLASKTGGGLTSLPANEATLSVRIERAI
                     KTWQGELPKSEQGYVFVLEDSETGTVAGICAIEVAVGLNDPWYNYRVGTLVHASKELN
                     VYNALPTLFLSNDHTGSSELCTLFLDPEWRKEGNGYLLSKSRFMFMAAFRDKFNDKVV
                     AEMRGVIDEHGYSPFWQSLGKRFFSMDFSRADFLCGTGQKAFIAELMPKHPIYTHFLS
                     QEAQDVIGQVHPQTAPARAVLEKEGFRYCNYIDIFDGGPTLECDIDRVRAIRKSRLVE
                     VAEGQPAQGDFPACLVANENYHHFRVVLVRTDPATERLILTAAQLDALKCHAGDRVRL
                     VRLCAEEKTA"
     gene            15384..16862
                     /gene="astD"
                     /locus_tag="J1Y83_002830"
     CDS             15384..16862
                     /gene="astD"
                     /locus_tag="J1Y83_002830"
                     /EC_number="1.2.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate-semialdehyde dehydrogenase"
                     /protein_id="HBA7630963.1"
                     /translation="MTLWINGDWVTGQGALRVKRNPVSGEVLWQGNDADAAQVGQACR
                     AARAAFPRWARLSFGDRQVRVERFAGLLESNKAELTAIIARETGKPRWESATEVTAMI
                     NKIAISIKAYHVRTGEQRSEMPDGAASLRHRPHGLLAVFGPYNFPGHLPNGHIVPALL
                     AGNTIIFKPSELTPWSGEAVMRLWQQAGLPPGVLNLVQGGRETGQALSALEDLDGLLF
                     TGSANTGYQLHRQLSGQPEKILALEMGGNNPLIIDEVADIDAAVHLTIQSAFVTAGQR
                     CTCARRLLLKSGTQGDAFLARLVAVSQRLTPGTWDDEPQPFIGGLISEQAAQQVVTAW
                     QELEAMGGRTLLAPRLLQAGTSLLTPGIIEMTGVTGVPDEEVFGPLLRVWRYDNFDEA
                     IRMANNTRFGLSCGLVSPEREKFDQLLLGARAGIVNWNKPLTGAASTAPFGGIGASGN
                     HRPSAWYAADYCAWPMASLESDSLTLPATLNPGLDFSDEVVR"
     gene            16859..18202
                     /gene="astB"
                     /locus_tag="J1Y83_002831"
     CDS             16859..18202
                     /gene="astB"
                     /locus_tag="J1Y83_002831"
                     /EC_number="3.5.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-succinylarginine dihydrolase"
                     /protein_id="HBA7630964.1"
                     /translation="MNAWEVNFDGLVGLTHHYAGLSFGNEASTRHRFQVSNPRLAAKQ
                     GLLKMKKLAGAGFPQAVIPPHERPFIPVLRQLGFSGSDEQVLEKVARQAPHWLSSVSS
                     ASPMWVANAATIAPSADTLDGKVHLTVANLNNKFHRSLEAPVTESLLKAIFNDEEKFS
                     VHSALPQVALLGDEGAANHNRLGGHYGEPGMQIFVYGREEGNDTRPSRYPARQTREAS
                     EAVARLNQVNPQQVIFAQQNPDVIDQGVFHNDVIAVSNRQVLFCHQQAFARQSQLLAN
                     LRARVSGFMAIEVPTAQVSVSDAVSTYLFNSQLLSRDDGSMMLVLPQECREHAGVWRY
                     LNELLAADNPISSLKVFDLRESMANGGGPACLRLRVVLTEEERRAVNPAVMMNDTLFN
                     ALNDWVDRYYRDRLTAADLADPQLLREGREALDTLTQLLDLGSVYPFQREGGGNG"
     gene            18195..19163
                     /gene="astE"
                     /locus_tag="J1Y83_002832"
     CDS             18195..19163
                     /gene="astE"
                     /locus_tag="J1Y83_002832"
                     /EC_number="3.5.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate desuccinylase"
                     /protein_id="HBA7630965.1"
                     /translation="MDNFLALTLTGKKPVITEREINGVRWRWLGDGVLELTPLTPPQG
                     ALVISAGIHGNETAPVEMLDALLGAISHGEIPLRWRLLVILGNPPALKQGKRYCHSDM
                     NRMFGGRWQLFAESGETCRARELEQCLEDFYDQGKESVRWHLDLHTAIRGSLHPQFGV
                     LPQRDIPWDEKFLTWLGAAGLEALVFHQEPGGTFTHFSARHFGALACTLELGKALPFG
                     QNDLRQFAVTASAIAALLSGESVGIVRTPPLRYRVVSQITRHSPSFEMHMANDTLNFM
                     PFEKGTLLAQDGEERFTVTHDVEYVLFPNPLVALGLRAGLMLEKIS"
BASE COUNT         4592 a         4896 c         5188 g         4538 t
ORIGIN      
        1 aaattacccc tttctttgcc agtaacttgt ctccactgga agaaatggaa aacgggaagg
       61 aaatggctac gatttatcaa tcattaaagg ataatgaaga gagcctgaaa tggtttgata
      121 tcgcagtatt aaataaagtt gctgaatttg aactatcaga caactatatg gcattgttaa
      181 tagagtgttt atcatattcc atgcgaaaag aaaaaatgat tcgcaagtgg aaattaacag
      241 aacgcttcaa taatttagca atgcaacatc atagtggata taaaaagctg ctaaatgcca
      301 taaaattaaa aggtcttttt tattatccat cttctgtata ccagtcctgc ttacatgctg
      361 cagagcaagg ctattcacta gcctggtatt ctcttggcaa aatgtattac gaaggtgatg
      421 aggtttcaca ggatctaaaa ttagccttta actggtttac ccgcgccgcg cagcataatg
      481 ttatcgatgc tctgtatgcg ctgggtatca tgtatagcga tggaaggggc acggataaga
      541 atatttgtga ggcacgaaaa tggtttttac ttgctgccca aaatggcaac acatctgcgc
      601 aatatgagct tgcccgtatc tcacgctttg ctgttgagcc attacgaaat tatgaagaag
      661 cattgcagtg gtatttatct gccgccacac aaggacatga ggttgcccag tatgagttgg
      721 gacaaatgta tatacaaggg attggcgtgg agcgcgatga ggttcaggcc catcgttggt
      781 ttttgcaatc agcagaacag ggatatttac atgcgcaata tcatactgcc aggttgtata
      841 gcggaagtga gagtattcca caggaccagg aaaaagcgct gtactggttt accaaagcag
      901 caaaaaatgg ggcggatggg gcaggggatg ccatgtatga attggggaaa tattatttaa
      961 ccaacaatga tgatcctgaa aataacgcag aggctactca atggcttact ggtgcagcgc
     1021 agcgtggtcg aatagaggcg atatttttgt tagccgaaat gtatctgtac ggtacaaaat
     1081 atatcgcaaa agatgaaaat catgcgctgc actggtacga aaaagctgcg cgtttagggg
     1141 cgaccgaagc gcaacaccag actgcggcaa tgtatgctca ggggacaggc actaaaattg
     1201 ataataaaca agcatggatg tggttaacaa tcgccggaaa taacaacccc tggattgaaa
     1261 aagatgactt gcgatgctta atgtctgaac aggatattca ggatgcagaa caaacggctg
     1321 caaattgcaa acgtctgcta aggatttaat taactcaggg aaaatcgtct ggttataagg
     1381 aaaaccagac gattgtaata acgattaaat cacgccctgg cttaacatcg catccgcaac
     1441 cttcacaaaa ccggcaatgt tcgcgccctg cacgtagttg gtttgctcac cttcaccacc
     1501 atgctcaaca caggcatggt ggatatccag catgatgtga tgcaaacgtg cgtcaacttt
     1561 ctcggctttc cagcccaggc gcgcagcgtt ttgcgccatt tccaggcccg atgttgctac
     1621 accgccagca ttagccgctt tacccggtgc aaacagtacg cctgtctgct ggaacagttc
     1681 agtcgcttcg atggtggtcg gcatatttgc cccttcggcg acggctttaa cgccattggc
     1741 gataagctga tgcgcggcgt caacatccag ttcgttctgg gttgcgcaag gcagggcgat
     1801 atcaaccggt acagaccacg gttgttggcc ttcgagatag accagaccaa attctttggc
     1861 gtaatctgcc actcgaccat cgcggctggc tttgatttcg ataagacgcg ccagtttttc
     1921 tttcgtgaat ccgctttcat caactacagt gccgctggag tctgacgcag tgatcactcg
     1981 agcaccaaat tccatcgctt tttcgatagc gtactgggcg acgttgccgg agccagaaac
     2041 ggaaacgcgc attccttcaa aacccatacc gtggcgtttt agcattgctt ctgtgaaata
     2101 aaccagaccg tagccggtag cttccgggcg aataaggctg ccgccaaatg aaaggccctt
     2161 accggtgaag acgcaggcgg tattgttgga gagctttttc atcatccccg ccataaagcc
     2221 gacttcacga ccaccaacgc cgatatcacc tgccggaacg tcagtatccg cgcccaggtg
     2281 gcgatacagt tcagtcatca gcgcctggca gaaacgcata acttcgcctt cgcttttccc
     2341 tttcggatca aagtcgctgc cgcctttacc accgcccatc ggcagggttg tcagggcatt
     2401 tttaaaggtt tgctcgaagc cgaggaattt gagaatggag aggttaactg acggatggaa
     2461 gcgcataccg cctttgtacg ggccgatggc agagctgaac tgcacacgcc atgcacggtt
     2521 gacctgtacc tggttgcgat cgtcaaccca taccacgcga aactggatca cgcgctccgg
     2581 ttcaaccaga cgctccagta atgacatctg gcgatatttt ggattttgtt caagaaaagg
     2641 ccagagtgtg gtcattactt cacgaacggc ttgcgcgaac tcggtttgat tcgggtcgcg
     2701 cttttggaca tggttgagga atgactccag agaatatgtc tgatccatag atataaaacc
     2761 cttatatatt aatacgattg tgcttatgtt gtttatgtca tgtgcttttg cagttttcga
     2821 ctataccatc aagaatgtga cggtaagcaa gaaaaaaatg acccaggaaa gcaaattaat
     2881 aacgagagta atctcataac caaatgcgat aactcagacg ctaaagtttt gccgcccggc
     2941 taccgttatc tgtagaagag acaataacgt taaggagttc cctgtgagtc gagcattgat
     3001 cgccgtagta ttagcatttc cattaattac actggctaac ccgcattatc atccggacgt
     3061 ggaggtaaac gtaccgccag aagtgttcag ttcaggcggg cagtcagcgc agccctgtac
     3121 tcagtgctgc gtttatcagg atcaaaatta ttctgaaggg gcggtaatta aagccgaagg
     3181 aatactgctg caatgccaac gggatgacaa aaaccttagc actaatccgc tggtctggcg
     3241 gcgcgtaaag ccataaacgc ctctaataat ggaatgtcag caggggccag cggatattgc
     3301 agcgcctctt caggtgagca ccagaccagc gcctgatgtt catgtgcctg taacgtcccg
     3361 tggaagtcgg gtacgtgcca ggcatgaaga tggataatcc gccccgaaac ttctcgctga
     3421 tggctggcaa catagtcacc aacagttgct tcgatgccca gttcttcgtt taactcacgc
     3481 accagcgcct gccgctggct ttcatccggc tcgactttac caccggcaaa ctcccataat
     3541 cccgcctgat cgctctgggc agggcgttgc gcgagtaaaa ttttgccatc acgttcgatg
     3601 atggcggcaa caacttcaat cattttcata aattacacac gggcagataa gcgaaaaatg
     3661 ccataatatc cgcactgttg ctcaatcaga aaggctttat atttaaggag tgcctgtgct
     3721 agaccgccat cttcatcccc ggattaaacc gttgttgcat cagtgcgtgc gggttcttga
     3781 taaaccgggc attacgccag atggtttaac attagtggga tttgccatcg gcgtgctggc
     3841 actgcctttt ctggcgctgg gctggtatct ggcggcgcta gtcgccattt tgttgaacag
     3901 gctgcttgat ggtctggacg gtgcgctggc gcggcgcaga gggcttaccg acgcgggcgg
     3961 ctttctcgat atttctctcg attttctttt ttacgcgctg gtgccttttg ggtttatcct
     4021 cgccgcaccg gagcaaaacg ccctggcagg cggctggctg ctgtttgcgt ttatcggcac
     4081 gggcagcagt tttctcgctt ttgctgcgct ggcggcgaaa catcagattg ataatcccgg
     4141 ttatgcgcat aaatcgtttt attatctggg cgggttaacc gaaggcagcg agacgatttt
     4201 gctgtttgtt ctgagttgcc tctttccggc ctgttttccg tggtttgcgt gggtatttgg
     4261 tgcgttgtgc tggatgacga cctttacgcg ggtgtggagt gggtatctga cgctgaagtc
     4321 gctccagcgt cagtaattgc aatgttattt actgctgtcc gggccgcgtt caccggttgc
     4381 taccggattt tttggatcgc tgctccattc gtaccagcca ccgtcgtaaa cggagacgtt
     4441 attccagccc attgcgcgtg cgtacataaa ggtttctgac gcgcgccagc cagtgccgca
     4501 gtagaatgaa acttgctgat ctggtttgat attccatgct ttccacatag cggttatatc
     4561 atcggcgctg cgcatggtgc catccgggtt atggaaatct tccatatgcg tcgagtcgct
     4621 acctgcgtgt ccccaacgtg ctccggctat ttcacctttt ggtttaatat agctgtaacc
     4681 gctggtcgta ccgataaatt ctggccacga acgaatgctc accagcgatg catcctggcg
     4741 atgcagcagt ccacgcgctt gttccatatc aagcatcagt tgcggttgtg ccgggatctt
     4801 cacgccgaaa tccggttccg ctttcacttt cggtggcgtt ccgcgctcaa cgggcagacc
     4861 cgcgtcggac caggtttgcc agcctccgtc cagcaggcgc acatctttca cgccggcata
     4921 aagcataatt tgcgccacac gcgctgcagc gtatacgtca cgcccgtaca gaatgaccgt
     4981 ggtgtcatgg cgaatgccgt gttttgccag catcgctttc agttgttcat cagaaacttt
     5041 gttccacagc ggttcgcttt ccacttcgtt ggtgtcgatg tagtcagcgc cgggaatatg
     5101 gctgataagg taaaattttg gagcgcccca ggccgcttca atgactttcc agtcaccggc
     5161 aggtttcgcc gtaacctctt taccttgttg caggtcatgc agccactgcg gatataccag
     5221 ctgttcaaag tgcggcagtt tttgcagacg ggaaggttcg cttagcgcgt cactcaggat
     5281 ggagatatgc gtaaaacctg ctttttgcag tcgcgttttg acggcgtcga catctttgtc
     5341 attaccgtac agcgccaccg gggcatcggc tttcaggtta tgttgcttga tccactcgtt
     5401 gagctgttcg gtgctcattt tgtcgagcca gctagcagag aggtttaagg cggcaggttc
     5461 atgaccggaa gggccattta aggtttgtgg ccagccgtta taaaacgcgc tggggcgagt
     5521 atcaatcgct ttgccatttt gttgttgaag ctggtcaagt gtaagaggct tcgccagttc
     5581 agcggcccac gaagaagcgc aagccagccc taaagccatt gccagcgcgg tcatttgaga
     5641 aacacgtttc atcgaaaatc ccgacattgt agttttgaga gcgccattct gaactgacgc
     5701 atcgggaaaa actttgcgtt atcgcagttt gttgtaattt tctgaccact gcgccatatc
     5761 cagaacgggg ctatcaggag gaacatcctg aagatcgtgc gtcacctgca caacagggat
     5821 cgccatggcg cgaatttcgc tgaacaccca atggcgaaaa ttatcgcgca gggccacatc
     5881 aagacggctg aatggctcat ccagtagcaa cgcttgtggc tgagcgagaa gggcgcgcag
     5941 cagagcaacg cgcgctcgct gaccgccaga caaagtggca ggatcctgat ggaacgctcc
     6001 gtcaaggcct gaacgctcaa gtgcatcatt cacggcatta cgtcgggcat tccccttaag
     6061 tgtcgccggt agcgccagca gtaaattttg cccgacgctg aactggtcaa ataacagtgc
     6121 atcctggaaa agaatgccaa tctgacgctg tgcggtgggt agcatgtcaa tccgttgctc
     6181 attaagccat agctcccctg tacaagaaaa ctgtccggcc agcgcaccaa tcatccacga
     6241 aaacagagtg gatttcccgc agccggatgg tcccattaac gtgacaatgt cacctttatc
     6301 taccgtaaag ttaacgtttg tcagcaagcg gctttctggt aaacgtagcg aaacattttt
     6361 cacgcagagc attagcggag tccttgtctg acataaccta cccattttgc gactaacgcg
     6421 gtcagggcaa aaataataag cggtaatagc agttgccata aagcctgggc ggcaagaata
     6481 ccgttgctgc cgccgctgct taatgccacc gcctcagtgg tgagcgtcgg aaaacgcccc
     6541 gcgcccagcc acagcgtcgg catatactgc gcaatactga ctgaaaatcc caccgcgaag
     6601 gcaatcagcg tagggcgtag cataagcgga catttcacgt aaaagaagat tttggaccgc
     6661 gaccagccca gtgtttgcgc aatcaaaatt aaccgtgaat caatgcgctg ccaggcgggt
     6721 tgcaggataa acagcatcca cggcattacc cacagcagat gcccccagac caccgccgtc
     6781 cagcttccat cctggttcag ccatagcgcc agcgtgtact ggcccgccac cagaggcaga
     6841 gcgggtaata aaatgggcaa ccatagccac aactggcgac gctgtagccc ccattccagc
     6901 cacagcagta acaggagcaa agcgatgaat gtcgccacca gccccattgt caggctgttg
     6961 atgagcgctt cactattgat cgtcgactga tccgcgagga tcgccagcag aaccacacac
     7021 agcacaccgg tcaagggtaa aaaactcgcc agcgaaatgc cgggcagtaa aggtgtggcg
     7081 ggcttgcgaa cgccatctac gcggggaata gtacgccgcc agccacgcca tagcagatag
     7141 cccagcaaaa cgtaggcggc gagtaacagc atcagcagca gactggcgag tgcgcctttc
     7201 gtttgttgat cggcgtcgcc ctgagttaac cactgccagc taattactgc cagcgtcggt
     7261 ggattacctg gcccaagaat aatcgccaca tccacgaccg acagcgacca ggcaacaatc
     7321 gccagcatcg ccattgccag cgcaggcgcg acggagggca acagcagcca gttcaggcat
     7381 tgccagcggc tgtagcccag cgaatccagc acaatgagtt gctgcaacag ccgtttttcg
     7441 ctcaatactg ccgctaagat ccacaataga aatgcgcttt ctttcaccgc cagcgtgaag
     7501 cccagcccaa tacccaattt gtccattggc ggagtgaaat atgggaaata gtcataaagc
     7561 agccctccgt cagcaaagag cagaagggcg ctggtggcaa aagccacatg gggaatggcg
     7621 agcagccacg gcagacgggc gcacatacgc tgccatttcg gccctggcca caacgccaca
     7681 atcaccagca gggcaatcaa caacgccccg accgccgcaa tggttgtcga ctccagcgtc
     7741 gccagtaatg cctgcggtaa ctgtggatcg gcaaataacg cctgccagtg cgtcaacgac
     7801 aaagcaggcg atatcagcgt gagagctgcc gggatcagcg gtgcataaat caccgccatc
     7861 atcgcccaca gcaaaaagat taatgcgtac cgtaacggtg tagccattct tgttccagcg
     7921 catttaccca acctgcgtgc ggttcagcca gtaccggcgg cagatcctgc ggcattctcg
     7981 attgcaatgt ttcgcgctgc ccgtcaggca gtttttgcgg atcgagaaca gaaggatcgc
     8041 cccagacagc gggatctgct ttacgcagtt gcgcatcggg tgagagcagg aaattggcaa
     8101 ctaccttcgc cgcagcactg gcattcgcgt tggcaggaat ggtgacgaaa tggacgttgc
     8161 caatcatccc ctcgcgaaaa ccaaaactgt aactgcttgc aggcaaatcg ccgctggcga
     8221 ttttttgctg cgcatgcgcg gggttaaagg tcagcgacag gcgcaatgtg tcggctttca
     8281 gcagagcatc catccgcgcg ggcgaaggcg ggaaatcctt tccttcgcgc cacaaatacg
     8341 gatgcagcgc atcaagatat tgccacaagg gagcagtgac acgggcgaaa gtcgcatcgt
     8401 ccggcgcttc ttttaatgcg gcgggatcgg gcgtcagcat aatcagcaac tgttcaagaa
     8461 acgccgtacc ggtaaagtcc ggtgggcgcg gataggtaac cgtgccggga ttggctttag
     8521 caaactccag taaggcttgc ggcgtttgtg gtggctgtgg cgtaacatcg cggcgggcga
     8581 taaacgtcag ttgtgcgccg ccccagggcg attccgcacc ttgtgtcggc actgaaaaat
     8641 cttcccgcac cggaagccgt gtgtcgacat agcgccagtt gggcagagtc tccgcccagc
     8701 ccgtttgtaa taaattggcc tcttttaagg tgcggaagtt ttcgccgttc acccagagca
     8761 gatccaccga accgcccgtt ttgcgtccgg cagcagcttc ggtctgaatg cgcttcaccg
     8821 cgtctgcggc atccgcgaga cgaacaatct tcaggtttat agcgtaatgg gttttcatct
     8881 cgccgctaac ccagtcgaga tagcggttaa ttgcggtatc gccgccccag gcgttaaacc
     8941 agacggtttg tcctttggcc tcatttttta tttcctgcca gtcactggca tgtgttgcca
     9001 aagaaaacag cgataacaat cccagcaacc acccacaatg gcgcatcttc tctccttaag
     9061 aacgtttttc aggaatacgg cacagcccct gagcgggaat gtccatggca gaattaaaac
     9121 gggctgacag attttgcagg cctatcaatg ccgtcagttc ggtgagcgcc tgagcgtcaa
     9181 aatgcgtagc cagtcgggcg cgcagggcat catcgaccgt tggcggcgtt acgcttgcgg
     9241 cttcggcata ctccagcgcc agccgttctt catcgctaaa gagcgggctt tgccgccagt
     9301 cagccactgc cagcagttta tcggtgctgc cggtacgctc ggcgactttc atactggtga
     9361 tatccacgca aaactcacac aggcacattt gcgcaatgcg cgcgctgaca agcgatcgta
     9421 ctaccggatc gagcggtgag cgtttgcgct ccagccagcc gacaaacatc gacaccagat
     9481 aaaagataaa cgggatccgc ccccaccagc gaatggggct tagcacctcg ccgtaatggc
     9541 gacgatgcag ccacgcctgc ggacgtaaaa tgaaaggaat tttgctaagc ggcggtaaac
     9601 ccatcaggca ttcctcttct ggcgctcaag ccatattttc ttcgccataa aaatcaccac
     9661 ggtcagggca aacagaataa acagtccggt gacaaaccag aaagtaccgc ctgttaacat
     9721 gctgcccgcc caggaataaa caatagtcgc cggtaattga ccaagcccgg tggcgataaa
     9781 aaacgagcga aaacgtattg aagtcaaacc ggcagcatag ctgattggat cgaaagggac
     9841 aaaaggcaat aaccgacata ccaggatggt gtgtttgccg taacgagtga aaaagccgtc
     9901 catactgtca agtacggttt tgccggttaa tttttccacc acttcgcggc ccatcactct
     9961 ggcgataaaa aagcacagtg ccgcgccggc catcgaactg gtccacgaca gtaagccgcc
    10021 ccagaacgca ccaaacagcg acgcattggc aaaggtgatc aaaaacgcag gtagcggtgc
    10081 agcaatggcc tgtaaaatca tcaacaagaa cgagacaact gccgctagtg cgccgtaaga
    10141 ctgaataaag cgttctatac cctgttggtc cacggcggca aacgccgcaa cgctgcgatt
    10201 gataaactca tggacgctgg gaagcagcgc ccatgccagc agcacagcga acaacaggat
    10261 gatgagggtt atacggtagt accagatttt acgcgattgc atcttcatca tccgttatcc
    10321 tgctttattc ggtgatttta aaggtaaccg ttgccagcgt gttatccgcc gggagaactg
    10381 aggcattgcc tttgaatttc acttcgccgc gtttttccac cgcaccataa gtgtatgttg
    10441 cattgctgac gatgccgacc gggcagctat ccagacacac caggcaaccc gttttcttct
    10501 cttctgctgc cgttaaatta ccgccaaagc gcatgtccag tttcttgcca ttactgtcaa
    10561 caatcacttc atcgaaggaa tacgcttttg ccgccccttg ccagttgacc gaaatatcca
    10621 gtttgctgcc tgtgacatga gtcgtttctt tattatccat cgtcatgttt tcgcccggcg
    10681 taccacctgc ctctttcagg gcttcataaa atgctttcgg ggtcgcataa cccataaaca
    10741 gcgatttatg tccgttggag ccatctttaa acacaatacc gtgacgagtg tcgtcggtga
    10801 ggaaacgacc attaatttgc accagcatgg ttaccgtatg gttattggca tcgacacgca
    10861 gcggttggct gttcgaaagt ccgtcgtatt ccacttttgc agccgcattc tctttttgat
    10921 cgcagccgct aagcccagca acagccagta agcagagtgc agccagtcct tttttccatg
    10981 acactgaata atgttgcaac atcgttctat ccttcatttt ttgggtgaga aagtgggctg
    11041 cggcgagaag cagacagatc cacatgtttg tgtcgggcgt agagttttgc gagctggacg
    11101 agaataaaca gtgccaggcc cgccagacag agttgtaaaa taaagcgcag cgtaatgcct
    11161 tcattggcga gatcgcttgc catcacggta taaataacaa taccgggcag ggtcgtgagt
    11221 gccgaaataa gggtataagg ccagaaggcg attgtggtta atccgtaagc gtaattttga
    11281 atattgtaag gaaacaacgg gattaagcgg gtcagaataa gaaaatcaat accgttacgc
    11341 gcaatgcctt tttcaatggc ctgaaaggta tggctatggc caacgtattt cagcagtaaa
    11401 tcacgtccca gccagcgcgc cagcaggaat gagcacgagg aggccagcgt ggcggcaatt
    11461 aatgatagta gtgtccctaa gagcgggcca aaaactattc ctcccgcgat caccagtatg
    11521 cttcctggta acagaaagag ggtggcaatg atgaataaca gaatatagag gctatagccg
    11581 aacaatccgc tctggcgaat gagtgtctgt aagtggggta aatcggtgag cagatcgaat
    11641 aaaccgaaag cgtggatagc gtaaatgacc agcgcaaaaa taagacaggc taaaagaaat
    11701 ttacgctcag cgttcatcgt ggagtttcct atatcaaact tgataataga gaagtggtca
    11761 gacaactaaa aacgcagtga cggtataaca aaggacggca ggcaacaaat cattactgac
    11821 agtttgttga tttttctcaa ggttaattct cctgacccag tttgagccag gagatcagct
    11881 aaattagcgg cggaaggtcg cccagacggg ggcgtgatcg gacggttttt ccatgctgcg
    11941 aatttcatag tcgatgccgg tttctacgca acattctgcc agcggttggc tggcgagcag
    12001 caggtcgatg cgcagaccgc gattatcgtc aaaacctttt gagcggtaat caaaccatga
    12061 gaaacgatct gctgtttgcg gattcgcatg gcggaaggta tcgatcaacc cccagctcat
    12121 cagcctctcc atccattcgc gttcctccgg aaggaaagaa cattttccgg tacgcagcca
    12181 gcgcttacgg ttctcttcgc caatgccaat atccaggtcg ccaggactga tattcatatc
    12241 gcccataatc agcaccggat tttcacgctt gagttcggtt tccaggtagt tttgcagatt
    12301 ctgataaaac tgcgcctttg ccgggaattt tatcggatgg tcgcggcttt caccctgcgg
    12361 gaagtaaccg ttgatcacgg tgacattgcc cagcggcgag gggatttccg ccataataat
    12421 ccgccgttgc gcttcttcgt cgtcaccagg aaagccgcga cgcacggcaa tcggcgtctc
    12481 tttggtcagc agcgccacgc cataatggcc tttctgcccg tgataaaaca cgttgtagcc
    12541 gagcttcgcc acctcttcga gcggaaacat atcgtcatga acttttgtct cctgcaggcc
    12601 aatcacatcc ggttggtgct tttcgacgat ggcttcaagc tggtgaggtc tggcgcgcag
    12661 gccgttgata ttaaaagaga caaatttcat agtcgctgcc atttattcag ataaagagtg
    12721 cgtggatggt agcagaattt cgctttgctt accgcctgtt gttaagtgat ttgatcgatg
    12781 gttgttaatg atgtcaacga tggcgcaaaa aatgcccgct tttggtgcgc gttgcgtcag
    12841 aatggcgcag taatttccag taaatttcgt caatgatcac aattccagaa ttatatttta
    12901 cttctgcata acattgcgtt ttttattctt tttattcacc cacatcgcaa acgtattcac
    12961 tttatatgca ctttaaatgc atatgatgtg gttataactg cttgatttcc agttagcctc
    13021 cgccgtttat gcacttttat cactggctgg cacgaaccct gcaatctaca tttacagcgc
    13081 aaacattact tattattaac atataaataa ctaattattt actgtagagg tcgctatgtc
    13141 tcagccaatt acgcgtgaaa actttgatga atggatgata cctgtttacg ctccggcacc
    13201 gtttataccg gtacgtggcg aaggttcacg cttgtgggat cagcagggga aagagtatat
    13261 cgacttcgcg ggtggcattg cggtgaacgc gctgggccat gcgcatccgg aactgcgtga
    13321 ggcgctgaac gaacaggcga gtaagttctg gcataccggc aacggttaca ccaacgagcc
    13381 ggtactgcga ctggcgaaaa aattgatcga cgccacgttt gccgatcgcg tcttcttttg
    13441 taactccggt gcggaagcca acgaagcggc actaaaactg gcgcgtaaat tcgctcacga
    13501 ccgctacggc agccataaga gcggcatcgt ggcgttcaaa aatgcgtttc atggtcgcac
    13561 gctgtttact gtcagtgcgg gtgggcagcc agcctattca caggatttta cgccactgcc
    13621 gccggatatt cgtcatgctg catataacga tattaactct gccagtgcgc tgattgacga
    13681 cgccacctgt gcggtgattg tcgaacccat ccagggggaa ggcggtgtgg tgccagccag
    13741 caacgcattt ttacaaggtc tgcgtgaatt gtgtgaccgc cacaatgcgt tgctgatctt
    13801 tgatgaagta caaaccggtg tcgggcgcac cggggaactg tatgcctata tgcactacgg
    13861 cgtgacgcct gatctgttaa ctaccgccaa agcgctgggc ggcggtttcc cggttggtgc
    13921 gttgttggca accgaagagt gcgccagcgt gatgaccgtt ggcactcacg gcaccaccta
    13981 tggcggcaat ccgctggcct cggcggtggc aggcaaagtg ctggatctca tcaacacgcc
    14041 agagatgctt aacggggtta aacagcgtca cgactggttt gttgagcgtc taaattccat
    14101 taatcaccac tacagtttgt tcagtgaagt tcgcggctta ggtcttttga ttggctgtgt
    14161 actgaatgcc gattacgccg ggcaagcgaa acagatctct caggaagcgg tgaaagcagg
    14221 cgtgatggtg ctgattgcgg gtggcaacgt ggtgcgcttt gcgcctgcgc tcaatgtcag
    14281 cgaagaagag gtgacgaccg gactggatcg ctttgcatct gcttgcgaac actttgttag
    14341 ccgaggttca tcatgatggt cattcgtccc gttgagcgct cagatgtctc ggcgctgatg
    14401 cagcttgcca gcaaaacggg cggcggcctg acgtcgcttc ccgccaatga agccacgctt
    14461 tcggtgcgta ttgaaagggc aatcaaaacc tggcaaggcg aactgcccaa aagtgagcag
    14521 ggttatgtgt ttgtactgga agatagtgag acaggcactg tggcggggat ttgcgccatt
    14581 gaggtggcgg ttgggctgaa cgatccctgg tacaactatc gtgtcggcac gctggttcac
    14641 gcttcgaaag agctgaatgt ctataacgca ttgccgacgc tgtttctcag taacgatcac
    14701 accggcagca gcgagctgtg cacgctgttt ctcgacccgg agtggcgcaa agagggcaac
    14761 ggctatttgc tgtcgaaatc gcgctttatg tttatggcgg cttttcgcga caagtttaat
    14821 gacaaagtgg tcgccgaaat gcgcggggtg attgacgaac acggctattc accgttctgg
    14881 caaagcctcg gtaaacgctt cttttcgatg gattttagcc gcgctgattt tctctgcggc
    14941 accgggcaaa aggcatttat tgcagaactg atgccgaaac atccgatcta tacccacttt
    15001 ttatcccagg aagcccagga cgtcatcggt caggtacatc cgcaaaccgc gcctgcccgt
    15061 gcggtgctgg agaaagaagg ttttcgctac tgtaactata tcgacatctt tgacggcggg
    15121 ccgacacttg aatgtgatat cgaccgcgtg cgcgccatcc gtaaaagtcg gctggtggaa
    15181 gtggccgaag ggcagcctgc gcagggcgat tttccggcct gcctggtcgc caatgaaaat
    15241 tatcaccatt tccgcgtggt gctggtgcgt accgatccag cgaccgagcg tttgatttta
    15301 accgccgcac aactggatgc cctcaaatgc cacgcagggg atcgcgttcg tctggtgcgc
    15361 ctgtgcgcag aggagaaaac agcatgactt tatggatcaa cggtgactgg gtaacgggcc
    15421 agggcgcatt gcgtgtgaag cgtaatccgg tatcgggcga ggtgttatgg cagggcaatg
    15481 atgccgatgc cgctcaggtc gggcaggctt gtcgggctgc ccgtgcggcg tttccgcgct
    15541 gggctcggct ctcatttggc gatcgtcagg taagagttga acgctttgcc ggattgctgg
    15601 aaagcaataa agccgaatta accgcgatta ttgccagaga aaccggcaag ccgcgctggg
    15661 aatcggcaac cgaagtgacg gcgatgatca ataaaatcgc gatatcaatt aaggcgtatc
    15721 acgttcgtac cggtgagcag cgtagcgaaa tgccggacgg cgcggcgagc ctgcgacatc
    15781 gcccgcacgg cttgctggcg gtgtttgggc cgtataattt ccctggtcat ttgccgaacg
    15841 gacatatcgt tccggcattg ctggcaggta acaccattat ctttaaaccc agcgaactga
    15901 caccgtggag tggcgaagcg gtaatgcgtt tatggcagca ggctggcttg ccgccgggcg
    15961 tgctgaacct ggtgcagggc gggcgtgaaa cgggtcaggc gctgagtgcg ctggaggatc
    16021 tcgacggctt gctgtttacc ggcagcgcca ataccggcta ccagctgcat cgccagcttt
    16081 ccggtcagcc ggagaaaatt ctcgcccttg agatgggcgg taataacccg ctaattatcg
    16141 atgaggtggc ggatatcgat gccgctgtcc atctgaccat tcagtcggcg tttgtcactg
    16201 ccgggcaacg ctgcacctgc gcccgccgtt tattgctgaa aagtggaacg cagggcgatg
    16261 catttcttgc tcgcctggtt gccgtcagcc agcggttaac gccgggcact tgggatgacg
    16321 aaccgcagcc atttattggt gggctgattt ctgagcaggc cgcacagcag gtggttactg
    16381 cctggcagga actggaggcg atgggcggac ggaccctgct tgcgccgcgc ttattacaag
    16441 cagggacatc gttgctgacg ccggggatca ttgaaatgac aggcgttact ggcgtgccgg
    16501 atgaagaagt gtttgggccg ttattgcgcg tctggcgtta tgacaatttc gatgaagcga
    16561 ttcgaatggc gaataacact cgcttcggcc tctcttgcgg tctggtttcc cccgagcggg
    16621 aaaaattcga tcaactgttg ctgggggcac gggcggggat tgttaactgg aacaaaccgc
    16681 ttaccggtgc tgccagtacc gcgccattcg gcggcattgg cgcttccggc aatcatcgcc
    16741 ccagcgcctg gtatgccgca gattattgtg cctggccgat ggcaagtctg gagtcggact
    16801 cgttaacgtt gcccgcaacg cttaaccccg ggctggattt ttccgatgag gtggtgcgat
    16861 gaacgcctgg gaagtcaatt tcgacgggct ggtagggctg acgcaccatt acgcgggcct
    16921 gtcgtttggt aatgaagcct ctacccgtca ccgttttcag gtgtctaacc cgcgactggc
    16981 ggcaaaacag ggcttactga aaatgaaaaa acttgccggt gcgggattcc cccaggccgt
    17041 gatcccgccg cacgagcgtc cgtttattcc ggtgctgcgt cagttgggat tcagtggtag
    17101 cgatgagcag gtactggaaa aagttgcacg ccaggcaccg cactggcttt ccagcgtcag
    17161 ttccgcttcg ccaatgtggg tagccaatgc ggcaacgatc gcgccatccg ccgatacgct
    17221 ggatggcaaa gtgcatctca ctgttgctaa cctgaacaat aaatttcacc gttcgctgga
    17281 agcccccgtc accgaatcgc tgttaaaagc gatttttaac gacgaagaga aatttagcgt
    17341 ccattcggcg ttgccgcagg tggctttgct cggtgacgaa ggagccgcga atcataatcg
    17401 tctcggcggt cattacggtg aaccgggaat gcagattttt gtttacgggc gagaggaggg
    17461 caatgatacc cgaccgtccc gctatccggc gcggcagact cgcgaagcca gcgaggcggt
    17521 ggcaaggctg aatcaggtga atccacagca ggtgattttc gcccagcaaa accccgacgt
    17581 tatcgaccag ggcgtttttc ataatgatgt gattgccgtg agtaaccgcc aggtgctgtt
    17641 ttgccatcaa caggcgttcg ctcgccagtc acagttactg gcaaatctgc gtgcacgggt
    17701 cagtggtttt atggcaatag aggtgcccac ggcgcaggtt tccgtgtcag atgcggtgtc
    17761 tacttatctg tttaacagcc aactgctgag ccgcgatgat ggttccatga tgttggtgct
    17821 gcctcaggaa tgtcgggaac atgctggagt atggcgttat ctcaatgaat tgcttgctgc
    17881 tgacaaccca ataagctcac taaaagtttt tgatttacgt gaaagcatgg cgaatggcgg
    17941 tggcccggcg tgcctgcggt tgcgcgtggt gttgacagaa gaagaacgcc gggcggtgaa
    18001 tccggcggtg atgatgaacg atacgctgtt taatgcgctc aatgactggg tggatcgtta
    18061 ctaccgcgat cgtcttactg cggcggatct tgccgatcca caattgctgc gcgaaggaag
    18121 ggaagcgctg gatacactga ctcaattatt ggatcttggt tcggtttatc cattccagcg
    18181 cgagggaggg ggcaatggat aattttcttg ctctaacctt aacgggtaaa aaaccggtta
    18241 tcaccgagcg agaaatcaac ggcgttcgct ggcgctggct gggcgatggt gtgctggaac
    18301 tgacgccatt aacgccaccg caaggcgcgc tggtgatttc agcggggatc cacggtaatg
    18361 agacggctcc tgtggaaatg ctggacgcgt tgcttggcgc gatatctcac ggtgagatcc
    18421 cgttacgttg gcggttactg gtgatcctcg ggaatcctcc tgcgctgaag caagggaaac
    18481 gttattgcca tagcgatatg aatcgaatgt ttggcggtcg ttggcagcta tttgctgaaa
    18541 gcggggaaac ctgtcgggcg cgcgaactgg agcagtgcct ggaagatttt tatgaccagg
    18601 gcaaagaatc tgtgcgctgg caccttgatc tacataccgc aattcgtggc tccttgcatc
    18661 cgcagttcgg tgtattaccg caacgcgaca ttccctggga cgagaaattt ctgacgtggc
    18721 tgggtgcggc ggggctggag gcgctggtgt tccatcagga gcctggtggt acgtttaccc
    18781 atttcagcgc cagacatttt ggcgcgctgg cctgtacgct ggaacttggc aaagcgttgc
    18841 cctttgggca aaacgatctt cgccagtttg cagtaactgc cagcgcaatt gctgcgctgc
    18901 tatctggtga gagtgtcggt atcgtgagaa caccgccgct ccgttatcgg gtggtttcgc
    18961 aaattactcg ccactcgccg tccttcgaaa tgcatatggc aaatgacacg ctgaatttta
    19021 tgccgtttga gaaaggaaca ttgctggcgc aggacgggga ggaacgtttt accgtaaccc
    19081 atgatgtaga gtatgtgtta ttccctaatc cgttggtagc gttgggatta cgtgcgggat
    19141 taatgctcga aaaaataagc taatacttac ccgcagaaat cattctgcgg gtattaagtt
    19201 gctttcttat aaat
//
LOCUS       DADSYM010000094        19201 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2415
            SAMN08039579-rid11723173.denovo.094, whole genome shotgun sequence.
ACCESSION   DADSYM010000094 DADSYM010000000
VERSION     DADSYM010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039579
            Sequence Read Archive: SRR6362533
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 19201)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19201)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980162.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 03:39:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,484
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 4,347
            CDSs (with protein)               :: 4,347
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 43 of 85
            Pseudo Genes (incomplete)         :: 37 of 85
            Pseudo Genes (internal stop)      :: 19 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19201
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039579-rid11723173.denovo.094"
                     /strain="LGE2415"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8302 N 95.9628 W"
                     /collection_date="2015-08-24"
     gene            413..1447
                     /gene="tqsA"
                     /locus_tag="J1Y83_002833"
     CDS             413..1447
                     /gene="tqsA"
                     /locus_tag="J1Y83_002833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2 transporter TqsA"
                     /protein_id="HBA7630966.1"
                     /translation="MAKPIITLNGLKIVIMLGMLVIILCGIRFAAEIIVPFILALFIA
                     VILNPLVQHMVRWRVPRVLAVSILMTIIVMAMVLLLAYLGSALNELTRTLPQYRNSIM
                     TPLQAIEPLLQRVGIDVSVDQLAHYIDLNAAMTLLTNLLTQLSNAMSSIFLLLLTVLF
                     MLLEVPQLPGKFQQMMARPVEGMAAIQRAIDSVSHYLVLKTAISIITGLVAWAMLAAL
                     DVRFAFVWGLLAFALNYIPNIGSVLAAIPPIAQVLVFNGFYEALLVLAGYLLINLVFG
                     NILEPRIMGRGLGLSTLVVFLSLIFWGWLLGPVGMLLSVPLTIIVKIALEQTAGGQSI
                     AVLLSDLNKE"
     gene            complement(1472..2860)
                     /gene="pntB"
                     /locus_tag="J1Y83_002834"
     CDS             complement(1472..2860)
                     /gene="pntB"
                     /locus_tag="J1Y83_002834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000014033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit beta"
                     /protein_id="HBA7630967.1"
                     /translation="MSGGLVTAAYIVAAILFIFSLAGLSKHETSRQGNNFGIAGMAIA
                     LIATIFGPDTGNVGWILLAMVIGGAIGIRLAKKVEMTEMPELVAILHSFVGLAAVLVG
                     FNSYLHHDAGMAPILVNIHLTEVFLGIFIGAVTFTGSVVAFGKLCGKISSKPLMLPNR
                     HKMNLAALVVSFLLLIVFVRTDSVGLQVLALLIMTAIALVFGWHLVASIGGADMPVVV
                     SMLNSYSGWAAAAAGFMLSNDLLIVTGALVGSSGAILSYIMCKAMNRSFISVIAGGFG
                     TDGSSTGDDQEVGEHREITAEETAELLKNSHSVIITPGYGMAVAQAQYPVAEITEKLR
                     ARGINVRFGIHPVAGRLPGHMNVLLAEAKVPYDIVLEMDEINDDFADTDTVLVIGAND
                     TVNPAAQDDPKSPIAGMPVLEVWKAQNVIVFKRSMNTGYAGVQNPLFFKENTHMLFGD
                     AKASVDAILKAL"
     gene            complement(2871..4403)
                     /gene="pntA"
                     /locus_tag="J1Y83_002835"
     CDS             complement(2871..4403)
                     /gene="pntA"
                     /locus_tag="J1Y83_002835"
                     /EC_number="1.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit alpha"
                     /protein_id="HBA7630968.1"
                     /translation="MRIGIPRERLTNETRVAATPKTVEQLLKLGFTVAVESGAGQLAS
                     FDDKAFVQAGAEIVEGNSVWQSEIILKVNAPLDDEIALLNPGTTLVSFIWPAQNPELM
                     QKLAERNVTVMAMDSVPRISRAQSLDALSSMANIAGYRAIVEAAHEFGRFFTGQITAA
                     GKVPPAKVMVIGAGVAGLAAIGAANSLGAIVRAFDTRPEVKEQVQSMGAEFLELDFKE
                     EAGSGDGYAKVMSDAFIKAEMELFAAQAKEVDIIVTTALIPGKPAPKLITREMVDSMK
                     AGSVIVDLAAQNGGNCEYTVPGEIFTTENGVKVIGYTDLPGRLPTQSSQLYGTNLVNL
                     LKLLCKEKDGNITVDFDDVVIRGVTVIRAGEITWPAPPIQVSAQPQAAQKAAPEVKTE
                     EKCACSPWRKYALMALAIILFGWMASVAPKEFLGHFTVFALACVVGYYVVWNVSHALH
                     TPLMSVTNAISGIIVVGALLQIGQGGWVSFLSFIAVLIASINIFGGFTVTQRMLKMFR
                     KN"
     gene            4927..5871
                     /locus_tag="J1Y83_002836"
     CDS             4927..5871
                     /locus_tag="J1Y83_002836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HBA7630969.1"
                     /translation="MKLKNTLLASALLSATAFSVNAATELTPEQAAAVKPFDRVVVTG
                     RFNAIGEAVKAVSRRADKEGAASFYVVDTSDFGNSGNWRVVADLYKADAEKAEETSNR
                     VINGVVELPKDQAVLIEPFDTVTVQGFYRSQPEVNDAITKAAKAKGAYSFYIVRQIDA
                     NQGGNQRITAFIYKKDAKKRIVQSPDVIPADSEAGRAALAAGGEAAKKVEIPGVATTA
                     SPSSEVGRFFETQSSKGGRYTVTLPDGTKVEELNKATAAMMVPFDSIKFSGNYGNMTE
                     VSYQVAKRAAKKGAKYYHITRQWQERGNNLTVSADLYK"
     gene            6057..7439
                     /locus_tag="J1Y83_002837"
     CDS             6057..7439
                     /locus_tag="J1Y83_002837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBA7630970.1"
                     /translation="MEKKLGLSALTALVLSSMLGAGVFSLPQNMAAVASPAALLIGWG
                     ITGAGILLLAFAMLILTRIRPELDGGIFTYAREGFGELIGFCSAWGYWLCAVIANVSY
                     LVIVFSALSFFTDTPELRLFGDGNTWQSIVGASALLWIVHFLILRGVQTAASINLVAT
                     LAKLLPLGLFVVLAIMMFKLDTFKLDFTGLALGVPVWEQVKNTMLITLWVFIGVEGAV
                     VVSARARNKRDVGKATLLAVLSALGVYLLVTLLSLGVVARPELAEIRNPSMAGLMVEM
                     MGPWGEIIIAAGLIVSVCGAYLSWTIMAAEVPFLAATHKAFPRIFARQNAQAAPSASL
                     WLTNICVQICLVLIWLTGSDYNTLLTIASEMILVPYFLVGAFLLKIATRPLHKAVGVG
                     ACIYGLWLLYASGPMHLLLSVVLYAPGLLVFLYARKTHTHDNVLNRQEMVLIGMLLIA
                     SVPATWMLVG"
     gene            7476..8198
                     /gene="folM"
                     /locus_tag="J1Y83_002838"
     CDS             7476..8198
                     /gene="folM"
                     /locus_tag="J1Y83_002838"
                     /EC_number="1.5.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydromonapterin reductase"
                     /protein_id="HBA7630971.1"
                     /translation="MGKTQSLPILITGGGRRIGLALAWHFINQKQPVIVSYRTHYPTI
                     DGLIKAGAQCIQADFSTNDGVMAFADEVLKSTHGLRAILHNASAWMAEKPGAPLTDVL
                     ACMMQIHVNTPYLLNHALERLLRGHGHAASDIIHFTDYVVERGSDKHIAYAASKAALD
                     NMTRSFARKLAPEVKVNSIAPSLILFNEHDDAEYRQQALNKSLMKTAPGEKEVIDLVD
                     YLLTSCFVTGRSLPLDGGRHLR"
     gene            complement(8195..8530)
                     /locus_tag="J1Y83_002839"
     CDS             complement(8195..8530)
                     /locus_tag="J1Y83_002839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlpM family protein"
                     /protein_id="HBA7630972.1"
                     /translation="MGLVIKAALGALVVLLIGVLAKTKNYYIAGLIPLFPTFALIAHY
                     IVASERGIEALRATIIFSMWSIIPYFVYLASLWYFTGMMRLPAAFVGSVACWGISAWV
                     LIICWIKLH"
     gene            8659..9378
                     /gene="rstA"
                     /locus_tag="J1Y83_002840"
     CDS             8659..9378
                     /gene="rstA"
                     /locus_tag="J1Y83_002840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416125.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RstA"
                     /protein_id="HBA7630973.1"
                     /translation="MNTIVFVEDDAEVGALIAAYLAKHDMQVTVEPRGDRAEETILRE
                     NPDLVLLDIMLPGKDGMTICRDLRAKWSGPIVLLTSLDSDMNHILALEMGACDYILKT
                     TPPAVLLARLRLHLRQNEQATVTKGIQETSLTPYKALHFGTLTIDPINRVVTLANTEI
                     SLSTADFELLWELATHAGQIMDRDALLKNLRGVSYDGLDRSVDVAISRLRKKLLDNAA
                     EPYRIKTVRNKGYLFAPHAWE"
     gene            9382..10683
                     /gene="rstB"
                     /locus_tag="J1Y83_002841"
     CDS             9382..10683
                     /gene="rstB"
                     /locus_tag="J1Y83_002841"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     RstB"
                     /protein_id="HBA7630974.1"
                     /translation="MKKLFIQFYLLLFVCFLVMSLLVGLVYKFTAERAGKQSLDDLMN
                     SSLYLMRSELREIPPHDWGKTLKEMDLNLSFDLRVEPLSKYHLDDISMHRLRGGEIVA
                     LDDQYTFLQRIPRSHYVLAVGPVPYLYYLHQMRLLDIALIAFIAISLAFPVFIWMRPH
                     WQDMLKLEAAAQRFGDGHLSERIHFDEGSSFERLGVAFNQMADNINALIASKKQLIDG
                     IAHELRTPLVRLRYRLEMSDNLSAAESQALNRDISQLEALIEELLTYARLDRPQNELH
                     LSEPDLPLWLSTHLADIQAVTPDKTVRIKTLAQGHYAALDMRLMERVLDNLLNNALRY
                     CHSTVETSLLLSGNRATLIVEDDGPGIAPENREHIFEPFVRLDPSRDRSTGGCGLGLA
                     IVHSIALAMGGTVNCDTSELGGARFSFSWPLWHNIPQFTSA"
     gene            10759..11688
                     /gene="tus"
                     /locus_tag="J1Y83_002842"
     CDS             10759..11688
                     /gene="tus"
                     /locus_tag="J1Y83_002842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication terminus site-binding protein"
                     /protein_id="HBA7630975.1"
                     /translation="MARYDLVDRLNTTFRQMEQELAAFAAELEQHKLLVARVFSLPEV
                     KKEDEHNPLNRIEVKQHLGNDAQSLALRHFRHLFIQQQSENRSSKAAVRLPGVLCYQV
                     DNFSQAALVSHIQHINKLKTTFEHIVTVESELPSAARFEWVHRHLPGLITLNAYRTLT
                     VLHDPATLRFGWANKHIIKNLHRDEVLAQLEKSLKSPRSVAPWTREEWQRKLEREYQD
                     IAALPQNAKLKIKRPVKVQPIARVWYKGDQKQVQHACPTPLIALINRDNGAGVPDVGE
                     LLNYDADNVQHRYKPQAQPLRLIIPRLHLYVAD"
     gene            complement(11685..13088)
                     /gene="fumC"
                     /locus_tag="J1Y83_002843"
     CDS             complement(11685..13088)
                     /gene="fumC"
                     /locus_tag="J1Y83_002843"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001099101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HBA7630976.1"
                     /translation="MNTVRSEKDSMGAIDVPADKLWGAQTQRSLEHFRISTEKMPTSL
                     IHALALTKRAAAKVNEDLGLLSEEKASAIRQAADEVLAGQHDDEFPLAIWQTGSGTQS
                     NMNMNEVLANRASELLGGVRGMERKVHPNDDVNKSQSSNDVFPTAMHVAALLALRKQL
                     IPQLKTLTQTLNEKSRAFADIVKIGRTHLQDATPLTLGQEISGWVAMLEHNLKHIEYS
                     LPHVAELALGGTAVGTGLNTHPEYARRVADELAVITCAPFVTAPNKFEALATCDALVQ
                     AHGALKGLAASLMKIANDVRWLASGPRCGIGEISIPENEPGSSIMPGKVNPTQCEALT
                     MLCCQVMGNDVAINMGGASGNFELNVFRPMVIHNFLQSVRLLADGMESFNKHCAVGIE
                     PNRERINQLLNESLMLVTALNTHIGYDKAAEIAKKAHKEGLTLKAAALALGYLSEAEF
                     DRWVRPEQMVGSMKAGG"
     gene            complement(13231..14877)
                     /locus_tag="J1Y83_002844"
     CDS             complement(13231..14877)
                     /locus_tag="J1Y83_002844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000066594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase"
                     /protein_id="HBA7630977.1"
                     /translation="MSNKPFHYQAPFPLKKDDTEYYLLTSEHVSVSEFEGQEILKVAP
                     EALTLLARQAFHDASFMLRPAHQQQVADILRDPEASENDKYVALQFLRNSDIAAKGVL
                     PTCQDTGTAIIVGKKGQRVWTGGGDEAALARGVYNTYIEDNLRYSQNAPLDMYKEVNT
                     GTNLPAQIDLYAVDGDEYKFLCIAKGGGSANKTYLYQETKALLTPGKLKNYLVEKMRT
                     LGTAACPPYHIAFVIGGTSAETNLKTVKLASAKYYDELPTEGNEHGQAFRDVELEKEL
                     LIEAQNLGLGAQFGGKYFAHDIRVIRLPRHGASCPVGMGVSCSADRNIKAKINRQGIW
                     IEKLEHNPGKYIPEELRKAGEGEAVRVDLNRPMKEILAQLSQYPVSTRLSLNGTIIVG
                     RDIAHAKLKERMDNGEGLPQYIKDHPIYYAGPAKTPEGYASGSLGPTTAGRMDSYVDQ
                     LQAQGGSMIMLAKGNRSQQVTDACKKHGGFYLGSIGGPAAVLAQGSIKSLECVEYPEL
                     GMEAIWKIEVEDFPAFILVDDKGNDFFQQIQLTQCTRCVK"
     gene            15076..16251
                     /gene="manA"
                     /locus_tag="J1Y83_002845"
     CDS             15076..16251
                     /gene="manA"
                     /locus_tag="J1Y83_002845"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416130.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase"
                     /protein_id="HBA7630978.1"
                     /translation="MQKLINSVQNYAWGSKTALTELYGMENPSSQPMAELWMGAHPKS
                     SSRVHDAAGDIVSLRDVIESDKATLLGDAVAKRFGELPFLFKVLCAAQPLSIQVHPNK
                     RNSEIGFAKENAAGIPMDAAERNYKDPNHKPELVFALTPFLAMNAFREFSEIVSLLQP
                     VAGAHPAIAHFLQQPDAERLSELFASLLNMQGEEKSRALAILKSALDSQQGEPWQTIR
                     LISEFYPEDSGLFSPLLLNVVKLNPGEAMFLFAETPHAYLQGVALEVMANSDNVLRAG
                     LTPKYIDIPELVANVKFEAKPANQLLTQPVKQGAELDFPIPVDDFAFSLHDLSDKETT
                     ISQQSAAILFCVEGDATLCKGSQQLQLKPGESAFIAANESPVTVRGHGRLARVYNKL"
     gene            16352..17860
                     /locus_tag="J1Y83_002846"
     CDS             16352..17860
                     /locus_tag="J1Y83_002846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdgA family protein"
                     /protein_id="HBA7630979.1"
                     /translation="MNKSLVAVGVIVALGVVWTGGAWYTGKKIETHLEDMVAQANAQL
                     KLTAPESNLEVSYQNYHRGVFSSQLQLLVKPIAGKVNPWIKSGQSVIFNESVDHGPFP
                     LAQLKKLNLIPSMASIQTTLVNNEVSKPLFDMAKGETPFEINSRIGYSGDSSSDISLK
                     PLNYEQKDEKVAFSGGEFQLNADRDGKAISLSGEAQSGRIDAVNEYNQKVQLTFNNLK
                     TDGSSTLASFGERVGNQKLSLEKMTISVEGKELALLEGMEISGKSDLVNDGKTINSQL
                     DYSLNSLKVQNQDLGSGKLTLKVGQIDGEAWHQFSQQYNAQTQALLAQPEIANNPELY
                     QEKVTEAFFSALPLMLKGDPVITIAPLSWKNSQGESALNLSLFLKDPATTKEAPQTLA
                     QEVDRSVKSLDAKLTIPVDMATELMTQVAKLEGYQEDQAKKLAKQQVEGASAMGQMFR
                     LTTLQDNTITTSLQYANGQITLNGQKMPLEDFVGMFAMPTLNVPAVPAIPQQ"
     gene            complement(17905..19170)
                     /gene="uidC"
                     /locus_tag="J1Y83_002847"
     CDS             complement(17905..19170)
                     /gene="uidC"
                     /locus_tag="J1Y83_002847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronide uptake porin UidC"
                     /protein_id="HBA7630980.1"
                     /translation="MRKIVAMAVICLTAASGLTSAYAAQLADDEAGLRIRLKNELRRA
                     DKPSAGTGRDIYAWVQGGLLDFNSGYYSNIIGVEGGVYYVYKLGARADMSTRWYLDGD
                     KSFGFALGAVKIKPSENSLLKLGRFGTDYSYGSLPYRIPLMAGSSQRTLRTVSEGALG
                     YWALTPNIDLWGMWRSRVFLWTDSTTGIRDEGVYNSQTGKYDKHRARSFLAASWHDDT
                     SRYSLGASVQKDVSNQIQSILEKSIPLDPNYTLKGELLGFYAQLEGLSRNTSQPNETA
                     LVSGQLTWNAPWGSVFGSGGYLRHAMNGAVVDTDIGYPFSLSLDRNREGMQSWQLGVN
                     YRLTPQFTLTFAPIVTRGYESSKRDVRIEGAGILGGMNYRVSEGPLQGMNFFLAADKG
                     REKRDGSTLGDRLNYWDVKMSIQYDFMLK"
BASE COUNT         4733 a         4961 c         4646 g         4861 t
ORIGIN      
        1 ttatccttct cctgcaagag aattatttta attttcgctt aattcagcga aaccaaagta
       61 aagtgatcaa gccccgcgca tagcgcaagc ttaggcagaa gaaaggacta aagcgcgttg
      121 cgccagtgct gctcacctac gagcaagata gtggatgagg tacgaagatg ggtaaatgta
      181 gaaaataacg tcctgaacaa attgtccata atattacaat tatccgcagt gttgcttctc
      241 gtcctcgcgg atgataattg tcctcggtag ttgaacacgc ctgatttgta tcatagctta
      301 agaattaact caaaatattt tcacttcttt acctgagcgg tttgattttc gttatgatga
      361 cggagcgaaa aagacattat tattagcaaa ggaagaaaaa acggggacaa gcatggcaaa
      421 gccgatcatc acgctcaatg gcctaaaaat cgtcattatg ttgggaatgc tggtcattat
      481 tctctgcggt atccgttttg ccgccgagat catcgtgccg tttattctcg cattatttat
      541 tgctgttatt cttaacccgc tggtgcaaca catggtccgc tggcgtgtgc cgcgtgtact
      601 ggcagtgtca attttgatga ccatcatcgt gatggcgatg gtgttgctgt tagcttatct
      661 gggttccgcg ctcaatgagt tgacgcggac gttaccgcaa tatcgcaact ctattatgac
      721 gccgctgcaa gcgattgaac cgttgttgca acgcgtaggg attgatgtct cagttgacca
      781 actggcgcat tacattgatc tgaacgcggc gatgacgttg ctcaccaact tattgacgca
      841 gttatctaat gccatgtcat caatattttt attgctgctg acggtgctgt ttatgttgct
      901 cgaagtgcca caattgcccg gaaaatttca gcaaatgatg gcgcgtccgg ttgaagggat
      961 ggcggcgatt cagcgtgcga ttgacagtgt gtctcattat ctggtgctga aaacagccat
     1021 cagcatcatc accggcctgg tcgcctgggc gatgctcgcc gcactcgatg ttcgcttcgc
     1081 ttttgtctgg ggattgctgg cctttgcgct taattacatc ccgaatattg gttcagttct
     1141 cgcggcaatc ccccctatcg ctcaggtact ggtgtttaat ggcttctacg aagcgttgct
     1201 ggtgctggcg ggatatctgc tgattaatct ggtcttcggc aatattctgg agccgcgcat
     1261 catgggacgt gggctggggc tttccacatt ggtggtattt ttgtcgttga ttttttgggg
     1321 atggttgtta ggaccggtgg gtatgctgct ttccgtgccg ttgacaatta ttgtcaaaat
     1381 tgcgcttgaa caaacagcgg gaggtcaaag catcgccgtt ctgttaagcg atctcaataa
     1441 agagtgacgg cctccgcaga ggccgtcagg gttacagagc tttcaggatt gcatccacgc
     1501 tggctttggc gtcaccaaac agcatgtggg tgttttcctt gaagaacagc gggttttgca
     1561 caccagcata gccagtgttc atcgaacgtt taaagacaat cacgttctgc gctttccaca
     1621 cttccagcac aggcatacca gcaatcggac tcttcggatc atcctgcgcc gccgggttaa
     1681 ccgtatcgtt agcaccaatc accagtacgg tatcggtatc agcaaagtca tcgttgatct
     1741 cgtccatttc cagcacgatg tcatacggta cttttgcttc agccagcaat acgttcatat
     1801 gtccaggcaa acgccccgca accgggtgga taccgaaacg cacgttgata ccacgagcgc
     1861 gcagtttctc ggtaatttca gcgacaggat attgcgcctg cgcgactgcc atgccgtacc
     1921 ccggagtaat gatcactgaa tgggagtttt tcagcagttc cgctgtctct tctgcggtga
     1981 tttcgcggtg ctcgcccact tcctgatcat caccagtaga agagccgtcg gtgccgaaac
     2041 cacccgcaat aacgctgata aaggaacggt tcatcgcctt acacataatg taagaaagga
     2101 tagcgcccga agaaccgacc agcgcaccgg tcacaatcag caggtcgttg ctaagcataa
     2161 agcccgcagc cgcagccgcc cagccggagt acgagttcag catcgacacc accactggca
     2221 tatctgcacc accgatggag gcgactaaat gccagccgaa taccagcgca atggcggtca
     2281 ttatcagcaa tgccagcact tgcaggccga cgctgtccgt gcgaacaaat acaatcagca
     2341 acaggaagga aacgaccaga gccgccaggt tcattttgtg acggtttggc agcatcaacg
     2401 gtttagacga aatcttgcca cacagtttgc cgaacgccac caccgacccc gtaaacgtta
     2461 ccgccccgat gaagataccg aggaacactt ccgtcaggtg aatattgacc agaatcggtg
     2521 ccattcccgc gtcatgatgc agatagctgt taaaaccaac cagcactgcc gccagaccca
     2581 cgaagctatg caggatcgcc accagttctg gcatttcggt catttcaact ttcttcgcca
     2641 gacggatacc aattgcccca ccaataacca tcgccagcaa gatccagcca acattacccg
     2701 tatccggtcc aaaaatggtt gcgattaacg caatcgccat accggcgata ccgaaattgt
     2761 taccctggcg agacgtttca tgtttcgaaa gcccggccag actgaagata aacaggatcg
     2821 cggcaacaat gtatgcagct gtaactaatc ctccagacat atgttacccc ttaatttttg
     2881 cggaacattt tcagcatgcg ctgagtcacg gtgaagccac cgaaaatatt aatgctggct
     2941 ataagcaccg cgataaaact aaggaagcta acccagccgc cctggccaat ctgcaacagt
     3001 gctccgacaa caataatccc tgaaatcgcg ttggtgaccg acatcaacgg tgtatgcagc
     3061 gcgtgcgata cattccacac cacgtaataa ccgacaacgc aggccagcgc gaacacagtg
     3121 aagtgtccaa ggaactcttt tggcgcaacg cttgccatcc agccgaaaag gatgattgcc
     3181 aacgccatca acgcgtattt acgccacggt gagcaggcac atttttcctc agttttcact
     3241 tccggtgccg ctttttgtgc cgcctgcggc tgagctgata cctgaatcgg cggtgccggc
     3301 caggtaattt cgcccgcacg gatcacggtc acgccacgaa tcaccacatc atcaaaatcc
     3361 acagtgatgt tgccgtcttt ctctttgcac aacagtttca gcagattaac gaggttagtg
     3421 ccgtaaagct gtgaggattg cgtcggcaga cggcccggaa gatcggtgta accaattact
     3481 ttgacaccat tttccgtagt gaagatttca cccggcacgg tgtattcaca gttgccgccg
     3541 ttttgggctg ccaggtcgac aatcacactg cccgccttca tggagtcaac catttcacgg
     3601 gtaattagct tcggcgctgg tttgcctgga ataagcgcgg tggtgacaat gatatcgacc
     3661 tcttttgcct gggcggcaaa gagttccatt tccgctttga tgaacgcgtc cgacatcact
     3721 ttggcatagc catcgccgct gcccgcttcc tctttaaaat ccagctcgag gaattctgcg
     3781 cccatacttt gaacttgttc tttcacttcc gggcgggtgt cgaatgcacg cacaatcgcg
     3841 ccgagactgt ttgctgcgcc aatggcggcc agacctgcga caccggcacc aatcaccatc
     3901 acttttgccg gtggcacttt cccggccgca gtgatttgtc cggtaaagaa gcgcccaaat
     3961 tcatgtgccg cttcaacaat ggcgcgataa ccggcgatat tcgccatcga gcttagtgcg
     4021 tccagcgatt gtgcgcgtga gatacgcggc acagagtcca tcgccatcac ggtcacgtta
     4081 cgttccgcaa gtttttgcat taattccgga ttctgcgcag gccagataaa actcaccagc
     4141 gttgtcccag gattcagtaa cgcaatttca tcatctaacg gcgcattgac cttcagaatg
     4201 atctctgact gccagacgct attcccttct acaatttcag cgcccgcttg cacaaacgct
     4261 ttatcgtcaa aacttgccag ttgacccgcg ccgctctcta ccgcgacggt aaaacccagt
     4321 ttcagcagct gttccactgt ttttggcgtt gctgcaacac gggtttcatt ggttaaccgt
     4381 tctcttggta tgccaattcg catgatattc ccttccatcg gttttattga tgatggtttg
     4441 cttgtgtcag gagccacaca agccgctcat gtacgagcta aatgttactc cgttaaaata
     4501 aattagtaac aaacgcccta taacgtactg aaaattatgc ctgtgatcta gcgccaaaaa
     4561 tcagtatttc ggcgtgaact cgcaaaatat taacgattca gccgtgatag tgggataaac
     4621 accttagaac gccggataaa gactgataat tgtcttcggc ggccgggtaa aacgagacat
     4681 cgccccggca caaatcacta cttaacatta aattaactta tacaattcag ttgcttcagt
     4741 agtattgatg gtgatacggc tgttttttaa gcatagacgg tcatttgagc agaattaaaa
     4801 ttggtttaag gaatgtgata tgaaaaatga cgcagacagt tacaccgttt aaatgcaata
     4861 atcagccacg tttctcgtta ataacaatac cagtacctgg tttgcgcaag gcgaaggatt
     4921 atttttatga agcttaagaa caccctcctg gcgtcggcac tgctttctgc tacggcattc
     4981 tccgttaacg cagcaacaga actgacaccg gagcaagcgg cagcggttaa accttttgac
     5041 cgtgtagtgg ttaccggtcg ttttaatgct attggcgaag cggtgaaagc cgtttctcgt
     5101 cgcgcagata aagaaggtgc cgcctctttt tatgttgtcg acacttctga ttttggtaac
     5161 agcggtaact ggcgtgtggt cgctgacctc tataaagccg atgctgaaaa agcagaagaa
     5221 acaagtaatc gcgtaattaa cggtgttgtc gaactgccga aagatcaggc tgttctgatt
     5281 gaaccgtttg acacggtcac cgtccagggc ttctatcgta gccagccaga agtcaatgat
     5341 gccatcacca aagcggcaaa agcgaaaggt gcctactctt tctacattgt tcgtcaaatc
     5401 gatgccaacc agggcggtaa ccagcgtatt actgcattca tctataaaaa agatgctaag
     5461 aaacgtatcg tccagagccc ggatgtaatc ccggcagatt ccgaagcagg acgtgcagct
     5521 ctggctgccg gtggcgaagc cgcgaagaaa gttgagatcc cgggggttgc gactaccgca
     5581 tcaccaagtt ctgaagtcgg tcgcttcttt gaaacccagt catcaaaagg cgggcgttac
     5641 accgtcacgc tcccggacgg cactaaagtc gaagaactga acaaagcgac agcagcgatg
     5701 atggtcccgt tcgacagcat caaattctct ggcaactacg gcaacatgac cgaagtctcc
     5761 tatcaggttg cgaaacgtgc cgcgaagaaa ggtgctaagt attaccacat cacccgccag
     5821 tggcaggaac gtggtaataa cctgaccgtc agcgcagatc tgtataaata actattcagt
     5881 tactcctata aggcggcttg atgccgcctt tcgcattttt agtcataaat ttccctgcga
     5941 ccattgcatg cagacacatc tcttcgtaaa atcccgcgct tcagtggcaa actccatttt
     6001 tatgcgtttt gccctaatat ttattcttct tgtcacgttt taatcactgg atatcgatgg
     6061 aaaagaaact gggactgagc gcactcaccg cgctggtatt aagctcaatg ctgggcgcgg
     6121 gtgttttcag tctgccgcaa aatatggcgg cagttgccag cccggcagca ctgctcatcg
     6181 gctggggtat tactggcgct ggcattttat tgctggcctt tgccatgctg atcctcacgc
     6241 gcattcgccc cgaacttgac ggcggtatct ttacctatgc ccgcgaaggg tttggcgagc
     6301 taatcggctt ttgttccgca tggggatact ggctgtgcgc ggtcatcgcc aacgtctcct
     6361 atctggtgat cgttttttcc gcgttaagct tttttacgga cacgccggaa ttgcgcctgt
     6421 ttggcgatgg taatacctgg caatcgatcg tcggtgcatc ggctttattg tggattgttc
     6481 actttttgat cctgcgcggg gtgcaaaccg ctgccagcat taaccttgtg gcgacactgg
     6541 caaaattgtt gccgttgggt ctgtttgttg tgctggcgat aatgatgttc aaactggata
     6601 ccttcaagct cgacttcacc ggacttgcac ttggcgtacc cgtgtgggaa caggtgaaaa
     6661 acaccatgct gatcaccctg tgggttttca ttggtgtgga aggtgccgtg gttgtttctg
     6721 cgcgtgcgcg taataagcgt gatgttggca aagcgacact gctggcggtt ctctccgctc
     6781 tgggcgttta tttgctggta acgctgcttt cgctgggcgt ggtggcacgt cctgaactgg
     6841 ctgaaattcg taacccgtca atggccggtc tgatggtgga aatgatggga ccgtggggcg
     6901 aaatcatcat cgctgccggt ttgatcgttt ccgtttgcgg cgcgtactta agctggacca
     6961 tcatggcagc agaagttccg ttccttgcag ccactcataa agcattcccg cgcatttttg
     7021 cgcgccagaa cgcacaagct gcgccatccg cttcactgtg gctgaccaac atctgcgtgc
     7081 aaatttgtct ggtgctcatc tggctgaccg gttccgatta caacacattg ctgaccatcg
     7141 cctcagaaat gattctggtg ccctatttcc tggtcggcgc gttcttgctg aaaatcgcaa
     7201 cccgtccact gcataaggcg gtaggtgtcg gtgcctgcat ttatggctta tggttactgt
     7261 acgcctctgg tccaatgcac ttgctgttat ccgttgttct gtatgcgccg gggctgctgg
     7321 ttttcctgta cgcgcgtaaa acgcatacac atgataatgt actgaaccgt caggaaatgg
     7381 tgctgattgg catgctgtta attgcctcag ttccggcgac ctggatgctg gtgggataac
     7441 gttcatccca tcgttggata gcaaaggaga taacgatggg taaaacccag tccttgccaa
     7501 tattaattac tggcggaggt cgccgcatcg gcctcgccct cgcatggcat ttcattaatc
     7561 aaaagcaacc ggtgattgtc agctatcgga cgcactatcc caccattgat ggactgatta
     7621 aggcaggtgc gcagtgtatt caggctgatt tttcgaccaa cgacggtgtg atggcgtttg
     7681 ccgatgaagt actaaaaagc acccatggtc tgcgtgctat tttgcataac gccagcgcgt
     7741 ggatggcgga aaaaccggga gcgccactga ccgacgtact ggcttgcatg atgcagatcc
     7801 acgttaatac cccatacctg ctcaaccatg ctctggaaag attactgcgt gggcacggac
     7861 acgccgccag cgatatcatt cactttaccg attatgtggt ggagcgcggt agcgacaaac
     7921 atattgccta tgctgcaagc aaagcggcac tggataatat gacccgctcg tttgcccgca
     7981 agctggcacc ggaagtgaaa gtgaattcta ttgcgccatc gctgatcctg tttaatgaac
     8041 atgatgatgc cgaatatcga caacaggcgc tgaataaatc actgatgaaa accgcgcctg
     8101 gcgagaaaga agtgatcgac ctggtcgatt acttacttac cagctgcttt gtcaccggac
     8161 gcagtctccc acttgatggc ggtcgtcatc tgcgttaatg cagcttaatc cagcatataa
     8221 tcaacaccca tgcgcttatt ccccagcacg caacagagcc aacaaaagca gcgggcaagc
     8281 gcatcattcc ggtgaaatac catagcgacg ccaggtaaac aaaataggga ataatcgacc
     8341 acatactaaa aatgatggtt gcgcgtaagg cttcaatgcc gcgttcgctg gcaacaatat
     8401 aatgcgcgat aagcgcaaag gtcgggaaaa gtggaatcag cccggcgata taataatttt
     8461 tcgtttttgc taaaacacca atcaacagca ctaccagcgc accgagcgcg gctttgatta
     8521 ccagccccat ctttttacct taacacttcc ataacaagtc atcagtagaa tacctgatga
     8581 aaccttgttt agaaacgatt gatagtaagt aaaaacagtg cggtgtattg tgacgttttt
     8641 atatctaccg tgaatgttat gaacactatc gtatttgtgg aagatgatgc ggaagtcggt
     8701 gcactgattg ccgcatacct ggcaaaacat gatatgcagg ttaccgtaga gccgcgcggc
     8761 gaccgggccg aagaaactat tctgcgagaa aatccggatt tggtgttact cgacatcatg
     8821 ctaccaggca aggacggcat gaccatttgt cgtgatttac gcgcaaagtg gtccggaccg
     8881 attgttcttc taacctctct cgatagcgat atgaaccaca tcctggcact ggaaatgggc
     8941 gcctgcgact atattctcaa aacgacgccc cctgctgttt tgctagcgcg tttacgtttg
     9001 catttgcgtc agaatgagca agccacagtg accaaaggta ttcaggaaac gtctctgact
     9061 ccctacaaag ccctgcattt cggcacgttg accatcgatc ccatcaaccg cgtagtcacc
     9121 ctggctaaca ctgaaatctc gctctcgaca gctgatttcg aattattgtg ggaattagct
     9181 acccatgccg ggcaaatcat ggaccgcgat gcattgctga aaaatttacg cggcgtcagt
     9241 tatgacggac tggatcgtag cgtggacgtg gctatttcgc ggttaagaaa aaaactgctc
     9301 gataacgccg cagaacctta tcgtattaaa accgtgcgta acaaaggcta tctttttgcg
     9361 cctcatgcgt gggaataagc gatgaaaaaa ctgtttatcc agttttacct gttattgttt
     9421 gtctgcttcc ttgtgatgtc tctgctggtc gggctggtgt acaaatttac cgccgaacgc
     9481 gcaggcaaac agtcgctgga tgatttgatg aacagttcgc tgtatctgat gcgcagcgaa
     9541 ttgcgtgaga tccccccaca cgactggggt aaaacgctga aagagatgga tttaaatctc
     9601 tctttcgatc tgcgtgtcga gccgctgagt aaatatcatc ttgatgatat ttccatgcac
     9661 cggctgcgtg gcggcgaaat tgtcgctctg gacgatcagt acactttttt gcaacgtatc
     9721 ccgcgaagcc actacgtgct ggcagttggt cctgttcctt atctttatta cctccatcag
     9781 atgcgattgc tggatatcgc cctgatcgct tttattgcca tttccctcgc ctttccggtg
     9841 tttatctgga tgcgtccgca ctggcaggat atgttaaaac tggaagcagc ggcgcaacga
     9901 tttggcgatg ggcatctcag tgagcgtatc cactttgatg aaggttcgag ctttgaacga
     9961 cttggcgtcg catttaacca gatggcggac aatatcaacg ccttgattgc cagcaaaaaa
    10021 cagcttattg acggtatcgc tcacgaactg cgaacaccgt tagtgcgcct gcgttatcga
    10081 ctggagatga gcgataacct gagcgccgcc gaatcccagg cgttgaatcg cgatatcagt
    10141 caacttgaag ctttaatcga agagctgctg acttatgccc gactcgaccg cccacaaaat
    10201 gagcttcatc ttagcgaacc agacctgccg ttgtggctgt caacgcatct ggcagatatt
    10261 caggcagtaa cgcccgataa aacggtacgg ataaaaacgc tcgcgcaagg ccattatgcg
    10321 gcgttggata tgcgcttaat ggagcgcgtg ctggataatt tgctcaataa cgccctgcgc
    10381 tactgtcact caacggttga aaccagcctg ctactgtcgg ggaatagagc gacattaatt
    10441 gttgaggatg atggcccagg gattgcccca gaaaaccgcg aacatatctt tgaacctttt
    10501 gttcgcctcg atcccagccg ggatcgctca accggcggct gcgggctggg gctggcaatt
    10561 gtccactcta tagcactggc aatgggcggt acggttaatt gtgacaccag cgaactgggt
    10621 ggtgcccgct tctcgttcag ctggccgtta tggcataaca tcccgcaatt tacctctgcc
    10681 tgacactatg cgcacggtgg tcaagtcacc accactgtgc tataaaataa ggatgttgta
    10741 actaaagtgg ttaatattat ggcgcgttac gatctcgtag accgactcaa cactactttt
    10801 cgccagatgg aacaagagct ggctgcattt gccgctgagc tagaacagca caaattgctg
    10861 gttgcccgcg tgttctcttt gccggaggta aaaaaagagg atgagcataa tccgcttaat
    10921 cgtattgagg taaaacaaca tctcggcaac gacgcgcaat cgctggcgtt gcgtcatttt
    10981 cgccatttat ttattcaaca acagtccgaa aatcgcagca gcaaggccgc tgtccgtctg
    11041 cctggcgtgt tgtgttacca ggtcgataac ttttcgcaag cagcgttagt cagtcatatt
    11101 cagcacatca ataaactcaa gaccacgttc gagcatatcg tcacggttga atcagaactc
    11161 ccctccgcgg cacgttttga atgggtgcat cgtcatttgc cggggctgat tacacttaat
    11221 gcttaccgca cgctcaccgt tctgcacgac cccgccactt tacgttttgg ctgggctaat
    11281 aaacatatca ttaaaaattt gcatcgtgat gaagtcctgg cacagctgga aaaaagcctg
    11341 aaatcaccac gcagtgtcgc accgtggacg cgcgaggagt ggcaaagaaa actggagcga
    11401 gaatatcagg atatcgctgc cctgccacag aacgcgaagt taaaaatcaa acgtccggtg
    11461 aaggtgcagc cgattgcccg cgtctggtac aaaggagatc aaaaacaagt acaacacgcc
    11521 tgccctacgc cgctgattgc actgattaat cgggataatg gcgcgggcgt gccagacgtt
    11581 ggtgagttgt taaattacga tgccgacaat gtgcagcacc gttataaacc acaggcgcag
    11641 ccgcttcgtt taatcattcc acggctgcac ctgtatgttg cagattaacc cccggctttc
    11701 atactgccga ccatctgttc tggccgtacc cagcggtcaa actcggcttc gctaagatac
    11761 cccagcgcaa gggccgccgc ttttaaggtc agcccttctt tatgcgcttt tttggcgatc
    11821 tcggcggctt tgtcataacc aatgtgggtg ttaagcgcag tcaccagcat cagcgattca
    11881 ttgagtaatt gattgattcg ctcacgattc ggttcaatgc ccactgcaca gtgtttgttg
    11941 aaactttcca tgccatctgc cagcaagcgc accgattgca ggaaattatg gatcaccatc
    12001 ggacggaaga cgttcagttc aaagttaccg gaagcgccac ccatgttgat cgccacgtcg
    12061 ttccccatca cctgacagca gagcatggtt aatgcttcgc actgtgttgg gttcactttc
    12121 cctggcatga ttgagctgcc cggctcattt tccgggattg agatttcacc aattccgcag
    12181 cgcgggccag aggccagcca gcggacatca ttggcaattt tcatcagtga cgcagccaac
    12241 cctttcaatg cgccgtgcgc ctgaaccagg gcatcacagg tcgccagcgc ttcaaatttg
    12301 ttcggcgcgg taacaaacgg tgcacaggta atgactgcca gttcatctgc tacgcgacgc
    12361 gcatattccg gatgggtatt tagtccagta cccaccgctg taccgcccag agccagttcc
    12421 gctacgtgag gcaggctgta ttcgatatgt ttgagattat gctccagcat cgctacccag
    12481 ccggaaatct cctgccccag cgttaacggc gtggcatcct gcaagtgagt acgaccaatt
    12541 ttgacgatat cggcaaatgc acgggatttc tcattcagtg tctgtgtcag ggttttaagc
    12601 tgcggaatga gttgcttgcg cagcgccagc agcgccgcaa cgtgcatcgc cgttggaaag
    12661 acatcgttag aactttggct tttgttcacg tcgtcgttag ggtgaacttt acgctccatc
    12721 ccacgcacgc cgccgagtaa ttcactggcc cggttagcca gcacttcgtt catgttcatg
    12781 ttactttgcg tgccggagcc ggtctgccag atagccagcg ggaattcatc gtcatgctgt
    12841 cctgccagta cttcatccgc cgcctgacga atggcgctcg ctttctcttc agacaacaag
    12901 cctaaatctt cattaacttt tgccgctgca cgcttggtta gcgccagcgc atgaatcagt
    12961 gaggtgggca ttttctccgt cgaaatgcgg aaatgctcca gcgagcgttg agtttgtgcg
    13021 ccccacagct tatctgccgg gacatcaatc gcccccatcg aatctttttc gctgcgtact
    13081 gtattcatgg cctgctcctc acctgattaa ttttttcttt ctgttttgct ttcgttaagc
    13141 aacttttagc tcactcatta tttactattt gataacaaat gtttggtctt tcgtgccatg
    13201 taaaaaaacc gccccgaagg gcggctctgt ttatttcaca cagcgggtgc actgtgtgag
    13261 ttgtatctgc tggaagaagt catttccttt atcatccaca aggataaacg ccgggaaatc
    13321 ttccacttca attttccaga tggcttccat tcccagttcc ggatattcaa cacattccag
    13381 gctcttaata ctaccctgcg ccaatacagc ggccggacca ccgatactgc caaggtagaa
    13441 gccgccgtgt tttttacagg catccgtcac ctgctggctg cggttgcctt tcgccagcat
    13501 gatcatactt ccgccctgcg cttgcagttg atcgacataa gaatccatcc gtccggcggt
    13561 cgttgggcca agagaaccgg aagcataacc ttccggcgtt ttggccggac ccgcgtagta
    13621 aatcggatga tctttgatgt actgcggcag cccttcaccg ttatccatcc gctctttcag
    13681 tttggcgtga gcaatatcac gaccgacgat aatcgtgccg ttaagcgata agcgtgtaga
    13741 aacgggatac tgcgacaact gtgcgaggat ctctttcatc ggacggttaa ggtcaacgcg
    13801 caccgcttcg ccctctcccg ctttgcgcag ctcttccggg atatatttgc ctggattatg
    13861 ttccagtttt tcgatccaga tcccctgacg gttgatcttc gctttgatat tacggtcagc
    13921 agaacaggag acgcccatac cgaccgggca ggatgcgccg tgacgtggca ggcgaatcac
    13981 gcggatgtcg tgagcgaagt atttaccacc aaactgcgca cccagaccaa gattttgcgc
    14041 ttcgatcagc aattcttttt ccagttccac atcgcggaac gcctgaccgt gctcattccc
    14101 ttccgttggc agttcatcat agtatttcgc ggaagccagt ttcaccgttt taaggttcgt
    14161 ttctgcagaa gttccaccaa taacgaacgc aatatgatac ggaggacagg ccgccgtacc
    14221 cagagtgcgc atcttctcaa ccaggtaatt tttcagtttc cccggcgtta gtaacgcttt
    14281 ggtttcctga tagagatacg tcttgtttgc cgaaccgcca cctttggcga tacagaggaa
    14341 tttgtactcg tcgccatcaa cggcataaag atcgatctgc gctggtagat tggtgccggt
    14401 attcacctct ttatacatat ccagcggcgc gttttgcgag tagcgcagat tatcttcgat
    14461 ataagtgtta tagacaccgc gtgccagcgc cgcttcatca ccgccaccgg tccatacacg
    14521 ctgtcctttt ttaccaacga taatcgcggt gccagtgtcc tgacaggttg gcagaacgcc
    14581 ttttgcggcg atatcggagt tgcgcaggaa ttgcagtgcc acatacttgt cgttttcgct
    14641 ggcttccggg tcacgcaaaa tatcggctac ctgctgttga tgtgccggac gcagcatgaa
    14701 tgacgcatca tgaaacgcct gacgcgccag aagagttaac gcttctggtg caactttcaa
    14761 aatctcctgc ccttcaaatt cagatacgct aacgtgttcg ctggttagca ggtaatactc
    14821 agtatcatct tttttgagtg gaaaaggagc ctgataatga aagggtttgt ttgacattgt
    14881 tctctcactt actgcctggt ttggttatgc tctgggcggg tgttccgttg ccctgttaaa
    14941 agcgagcaac aatatcctac acactttttt aacaaaaact gagactagta cgactttttg
    15001 cggctccagg ttacttcccg taggattctt gctttaatag cgggattaat ttccacatta
    15061 aaacagggat tgatcatgca aaaactcatt aactcagtgc aaaactatgc ctggggcagc
    15121 aaaacggcgt tgactgaact ttatggtatg gaaaatccgt ccagccagcc gatggccgag
    15181 ttgtggatgg gcgcgcatcc aaagagcagt tcacgtgtgc acgatgccgc aggagatatc
    15241 gtttcactgc gtgatgtgat tgagagtgat aaagcgactc ttttgggaga tgccgttgcc
    15301 aaacgctttg gcgagttgcc tttcctgttc aaagtattat gcgcagccca gccgctctcc
    15361 attcaggttc atccaaacaa acgcaattct gaaatcggtt ttgccaaaga aaatgctgca
    15421 ggtattccga tggatgccgc tgagcgtaac tataaagatc ctaatcacaa gcctgagctg
    15481 gttttcgccc tgaccccttt cctcgcgatg aacgcgtttc gtgagttttc cgagattgtc
    15541 tccctgctcc agccagtcgc aggtgcacat ccggcgattg ctcacttttt acaacagcct
    15601 gatgccgaac gtttaagcga actgttcgcc agcctgttga atatgcaggg tgaagaaaaa
    15661 tcccgcgcgc tggcgatttt aaaatcggcc ctcgacagcc agcagggtga accatggcaa
    15721 acgattcgtt taatttctga attttacccg gaagacagcg gtctgttctc cccgctgttg
    15781 ctgaatgtgg tgaaattgaa tcctggcgaa gcgatgttcc tgttcgctga aacaccgcac
    15841 gcttacctgc aaggcgtggc gctggaagtg atggcgaact ccgataacgt gctgcgtgca
    15901 ggtctgacgc ctaaatacat tgatattccg gaactggttg ccaacgtgaa gttcgaagcc
    15961 aaaccggcta accagttgtt gacccagccg gtaaaacaag gtgcagaact ggacttcccg
    16021 attcctgtgg atgattttgc cttctcgttg catgacctta gtgataaaga aaccaccatc
    16081 agccagcaga gtgccgccat tttgttctgc gtcgaaggcg atgcaacgct gtgtaaaggt
    16141 tctcagcagt tacagcttaa accgggtgaa tcagcgttta ttgccgccaa cgaatcaccg
    16201 gtgactgtca gaggccacgg ccgtttagcg cgtgtttaca acaagctgta agagcttact
    16261 gaaaaaatta acatctcttg ctaagcttat taaaggctta taacaccttc aggcggccag
    16321 tccgcctgat ttcattttat ggataatcat tatgaataaa tcgctggtag cggtaggcgt
    16381 cattgttgcg ctaggcgtag tctggacagg cggcgcatgg tatacaggca agaagattga
    16441 aacccatctc gaagacatgg tcgcgcaggc gaacgcgcaa ctcaaactga cagctcctga
    16501 atccaacctg gaagtcagtt atcaaaacta tcatcgcggc gtattcagca gccagttgca
    16561 actgttggtg aaacccattg ccggaaaagt aaatccgtgg attaaaagcg gtcagagcgt
    16621 catcttcaac gaatcggttg atcatggtcc cttcccgctt gcccagctta aaaaactgaa
    16681 cctgatcccg tcgatggcat caattcaaac cacgctggtt aataacgaag taagcaaacc
    16741 actgtttgat atggcaaaag gtgaaacgcc ttttgagatt aactcccgca ttggttacag
    16801 cggagattcc agttccgaca tttcgctcaa gccactgaat tacgagcaaa aggatgaaaa
    16861 agtcgccttt agcggcggcg agttccagtt aaatgcagac agagacggca aagctatctc
    16921 cctttccggg gaggcgcaaa gtggtcggat agacgcggtt aacgaataca accagaaagt
    16981 gcagttaacc tttaataatc tgaaaaccga cggttccagc acgctggcaa gttttggtga
    17041 gcgcgtagga aaccaaaaac tgtcgctgga aaaaatgacc atttcagtgg agggcaaaga
    17101 actggcactg ctggaaggca tggagatcag cggtaaatcg gatctggtca atgacggtaa
    17161 aacgatcaat agccaactgg attactcgct aaacagcctg aaggtacaga atcaggattt
    17221 gggcagtggc aagctgactt taaaagtcgg ccaaattgat ggcgaagcct ggcatcagtt
    17281 tagccagcaa tataacgcgc aaactcaggc gctgctggca cagccagaaa ttgccaacaa
    17341 tcccgaactt tatcaggaga aagtgacgga agccttcttt agcgctctgc cgctgatgct
    17401 gaaaggcgat ccggtgatta ctatcgcgcc gctaagctgg aaaaacagtc agggtgaaag
    17461 tgcgctgaat ctgtcgctgt tcctgaaaga tccggcaacg actaaagaag cgccgcaaac
    17521 gctggcgcag gaagtggacc gctcggttaa atctctggat gcgaaactga ccattccggt
    17581 ggatatggca actgagttaa tgactcaggt agcgaagctg gaaggttatc aggaagatca
    17641 agcgaaaaaa ctggcgaaac agcaagttga aggtgcatca gcaatggggc agatgttccg
    17701 tctgaccacc ttgcaggaca ataccatcac caccagcctg caatatgcta acggtcagat
    17761 aacgttaaac gggcagaaaa tgccactgga agatttcgtg ggtatgtttg cgatgccgac
    17821 attaaacgtt ccggctgtac ccgctattcc gcagcagtaa ttcacagcca aaacgccgga
    17881 gatttttctc tccggcgttt tttcttactt cagcataaag tcatactgaa tactcatttt
    17941 cacatcccag taattcaggc gatcgcccag cgtactgcca tcgcgctttt cccgcccttt
    18001 atcggcagca agaaagaaat tcatcccttg taacggccct tcgctgaccc gatagttcat
    18061 accccctaag atacccgcgc cttcaatccg cacatctcgt ttactggatt catagccgcg
    18121 ggtcacaatc ggtgcaaatg tcagcgtaaa ttgcggagtt aaacgatagt tgacgcccaa
    18181 ttgccaggac tgcattcctt cacggttacg atcaagactt aatgaaaagg gatagccaat
    18241 gtcggtatcc accacggcac cattcattgc atggcgcaaa taaccaccac tgccaaatac
    18301 gcttccccac ggcgcattcc aggtcaattg tccactaacc aacgccgttt cattgggctg
    18361 gctggtatta cgacttaaac cttcaagttg cgcgtaaaag ccgagcaact cccctttcaa
    18421 cgtataattc gggtcaagcg gtatgctttt ctcgagaata ctttgtatct gattggaaac
    18481 atctttctgt accgatgccc ccagagaata gcgactggta tcatcatgcc aactggcggc
    18541 taaaaaagaa cgtgcgcgat gtttatcgta ttttcccgtc tggctgttat aaacaccttc
    18601 atcacgaata ccggttgttg aatcggtcca taaaaatact cgtgaacgcc acattcccca
    18661 cagatcaata tttggcgtta aagcccaata acctaatgct ccttcagaaa cagtccgtaa
    18721 tgtacgttgc gaactgccag ccattaacgg aatacgataa ggtaagctac cataactata
    18781 atccgtcccg aagcgaccta attttagcag gctattttca ctgggtttta tttttactgc
    18841 ccccagggca aagccaaaac ttttatcacc atcaagatac caccgggtac tcatatcagc
    18901 acgagcacct aatttataaa cataatacac cccgccttca acgccaataa tattggaata
    18961 ataaccacta ttgaaatcga gcaatcctcc ctgtacccat gcgtaaatat ctcttcccgt
    19021 gccagcactg ggcttatcag ccctgcgcaa ttcgtttttc agtctgatgc gtagtcccgc
    19081 ttcatcgtca gccagttgcg ccgcgtaagc agaggtaagg ccagaggcag ccgtcaggca
    19141 aataacggcc atggccacta tttttctcat agttgcacct ttgatgttct gattattttt
    19201 a
//
LOCUS       DADSYM010000095        19095 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2415
            SAMN08039579-rid11723173.denovo.095, whole genome shotgun sequence.
ACCESSION   DADSYM010000095 DADSYM010000000
VERSION     DADSYM010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039579
            Sequence Read Archive: SRR6362533
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 19095)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19095)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980162.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 03:39:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,484
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 4,347
            CDSs (with protein)               :: 4,347
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 43 of 85
            Pseudo Genes (incomplete)         :: 37 of 85
            Pseudo Genes (internal stop)      :: 19 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19095
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039579-rid11723173.denovo.095"
                     /strain="LGE2415"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8302 N 95.9628 W"
                     /collection_date="2015-08-24"
     gene            334..1473
                     /locus_tag="J1Y83_002848"
     CDS             334..1473
                     /locus_tag="J1Y83_002848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide export protein"
                     /protein_id="HBA7630981.1"
                     /translation="MMKSKMKLMPLLVSVTLISGCTVLPGSNMSTMGKDVIKQQDADF
                     DLDKMVNVYPLTPRLIDQLCPRPNVARPNMTLESEIANYQYRVGPGDVLNVTVWDHPE
                     LTTPAGQYRSSSDTGNWVQPDGTMFYPYIGKVHVVGKTLAEIRSDITGRLATYIADPQ
                     VDVNIAAFRSQKAYISGQVNKSGQQAITNVPLTILDAINAAGGLTDTADWRNVVLTHN
                     GREERISLQALMQNGDLNQNRLLYPGDILYVPRNDDLKVFVMGEVKKQSTLKMDFSGM
                     TLTEALGNAEGIDMTTSNASGIFVIRPLKGESGRNGKIANIYQLDMSDATSLVMATEF
                     RLQPYDVVYVTTAPVSRWNRLINQLLPTISGVRYMTDTASDIHNW"
     gene            1479..1922
                     /gene="wzb"
                     /locus_tag="J1Y83_002849"
     CDS             1479..1922
                     /gene="wzb"
                     /locus_tag="J1Y83_002849"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight
                     protein-tyrosine-phosphatase Wzb"
                     /protein_id="HBA7630982.1"
                     /translation="MFNNILVVCVGNICRSPTAERLLQRYHPELKVESAGLGALVGKG
                     ADPTAISVAAEHQLSLEGHCARQISRRLCRNYDLILTMEKRHIERLCEMAPEMRGKVM
                     LFGHWDNECEIPDPYRKSRETFAAVYTLLERSARQWAQALNAEQV"
     gene            1925..4087
                     /gene="wzc"
                     /locus_tag="J1Y83_002850"
     CDS             1925..4087
                     /gene="wzc"
                     /locus_tag="J1Y83_002850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein kinase Wzc"
                     /protein_id="HBA7630983.1"
                     /translation="MTEKVKQHAAPVTGSDEIDIGRLVGTVIEARWWVIGITAVFALC
                     AVVYTFFATPIYSADALVQIEQSSGNSLVQDIGSALANKPPASDAEIQLIRSRLVLGK
                     TVDDLDLDIAVSKNTFPIFGAGWERLMGRQNETVKVTTFNRPKEMADQVFTLNVLDNK
                     NYTLSSDGGFSARGQAGQMLKKEGVTLMVEAIHASPGSEFTVTKYSTLGMINQLQNSL
                     TVTENGKDAGVLSLTYTGEDREQIRDILNSIARNYQEQNIERKSAEASKSLAFLAQQL
                     PEVRSRLDVAENKLNAFRQDKDSVDLPLEAKAVLDSMVNIDAQLNELTFKEAEISKLY
                     TKVHPAYRTLLEKRQALEDEKAKLNGRVTAMPKTQQEIVRLTRDVESGQQVYMQLLNK
                     EQELKITEASTVGDVRIVDPAITQPGVLKPKKGLIILGAIILGLMLSIVGVLLRSLFN
                     RGIESPQVLEEHGISVYASIPLSEWQKARDSVKTIKGIKRYKQSQLLAVGNPTDLAIE
                     AIRSLRTSLHFAMMQAQNNVLMMTGVSPSIGKTFVCANLAAVISQTNKRVLLIDCDMR
                     KGYTHELLGTNNVNGLSEILIGQGDITTAAKPTSIAKFDLIPRGQVPPNPSELLMSER
                     FAELVKWASKNYDLVLIDTPPILAVTDAAIVGRHVGTTLMVARYAVNTLKEVETSLSR
                     FEQNGIPVKGVILNSIFRRASAYQDYGYYEYEYKSDAK"
     gene            4180..5019
                     /gene="wcaA"
                     /locus_tag="J1Y83_002851"
     CDS             4180..5019
                     /gene="wcaA"
                     /locus_tag="J1Y83_002851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaA"
                     /protein_id="HBA7630984.1"
                     /translation="MKDNPLISIYMPTWNRQQLAIRAIKSVLRQDYNNWEMIIVDDCS
                     TSWEQLQQYVTALNDPRITYIHNDINSGACAVRNQAIMLAQGEYITGIDDDDEWIPNR
                     LSVFLAHKQQLITHAFLYANDYVCQGEVYSQPASLPLYPKSPYSRRLFYKRNIIGNQV
                     FTWAWRFKECLFDTELKAAQDYDIFLRMVVEYGEPWKVEEATQILHINHGEMQITSSP
                     KKFSGYFHFYRKHKDKFDRASKKYQLFTLYQIRNKRMTWRTLLTLLSVRNGKRLADGI
                     RGR"
     gene            5022..5510
                     /gene="wcaB"
                     /locus_tag="J1Y83_002852"
     CDS             5022..5510
                     /gene="wcaB"
                     /locus_tag="J1Y83_002852"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis acetyltransferase
                     WcaB"
                     /protein_id="HBA7630985.1"
                     /translation="MLEDLRANSWSLRPCCMVLAYRAAHFCSVWRKKNVLNNLWAAPL
                     LVLYRIITECFFGYEIQAAATIGRRFTIHHGYAVVINKNVVAGDDFTIRHGVTIGNRG
                     ADNMACPHIGNGVELGANVIILGDITLGNNVTVGAGSVVLDSVPDNALVVGEKARVKV
                     IK"
     gene            5507..6724
                     /gene="wcaC"
                     /locus_tag="J1Y83_002853"
     CDS             5507..6724
                     /gene="wcaC"
                     /locus_tag="J1Y83_002853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaC"
                     /protein_id="HBA7630986.1"
                     /translation="MNILQFNVRLAEGGAAGVALDLHQRALQQGLASHFVYGYGKGGK
                     ESVSHQNYPQVIKHTPRMTAMANIALFRLLNRDLFGNFNELYRTITRTPGPVVLHFHV
                     LHSYWLNLKSVVRFCEKVKNHKPDVTLVWTLHDHWSVTGRCAFTDGCEGWKTGCQKCP
                     TLNNYPPVKIDRAHQLVAGKRQLFREMLALGCQFISPSQHVADAFNSLYGPGRCRIIN
                     NGIDMATEAILADLPPVRETQDKPKIAVVAHDLRYDGKTNQQLVREMMALGDKIELHT
                     FGKFSPFTAGNVVNHGFETDKRKLMSALNQMDALVFSSRVDNYPLILCEALSIGVPVI
                     ATHSDAAREVLQKSGGKTVSEEDVLQLIQLSKPEIAQAIFGTTLAEFSQRSRAAYSGQ
                     QMLEEYVNFYQNL"
     gene            6699..7916
                     /gene="wcaD"
                     /locus_tag="J1Y83_002854"
     CDS             6699..7916
                     /gene="wcaD"
                     /locus_tag="J1Y83_002854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative colanic acid polymerase WcaD"
                     /protein_id="HBA7630987.1"
                     /translation="MSTSIRICSYLLLPLIYLLVNVKIAQLGESFPITIVTFLPVLLL
                     LFLERISVKKLMIALGIGAGLTAFNYLFGQSLDASKYVTSTMLFVYIVIIIGMVWSIR
                     FKTISPHNHRKILRFFYLVVGLVVALAAVEMAQIILTGGSSIMESISKYLIYSNSYVL
                     NFIKFGGKRTTALYFEPAFFALALISIWLSIKQFGIKTPKTDAMILAGIILSGSFSGV
                     MTFILFYLLEWAFQYLNKEAIKKKLPLALISLAVFLVGVVIAFPYISTRLGDLGTEGS
                     SSYYRIVGPLVMVGYSLTHIDGVVRFGSLYEYVASFGIFNGADVGKTIDNGLYLLIIY
                     FSWFAVFLSLWYMGKVIKMMINAFGDNRNFRVQLYLFTPVSLFFTGSIFSPEYAFLIV
                     CPFILRKALNITR"
     gene            7927..8673
                     /gene="wcaE"
                     /locus_tag="J1Y83_002855"
     CDS             7927..8673
                     /gene="wcaE"
                     /locus_tag="J1Y83_002855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaE"
                     /protein_id="HBA7630988.1"
                     /translation="MLLSIITVAFRNLEGIVKTHASLAHLAQADDISFEWIVVDGGSN
                     DGTREYLENLNGIYNLRFVSEPDNGIYDAMNKGIAMAQGKFALFLNSGDIFHQDAANF
                     VRKLKMQKDNMMITGDALLDFGDGHKIKRNAKPGWYIYHSLPASHQAIFFPVSGLKKW
                     RYDLEYKVSSDYALAAKMYKAGYAFKKLNGLVSEFSMGGVSTTNNMELCADAKKVQRQ
                     ILHVPGFWAELSWHLRQRTTSKTKALYNKS"
     gene            8689..9237
                     /gene="wcaF"
                     /locus_tag="J1Y83_002856"
     CDS             8689..9237
                     /gene="wcaF"
                     /locus_tag="J1Y83_002856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis acetyltransferase
                     WcaF"
                     /protein_id="HBA7630989.1"
                     /translation="MQDLSGFSVPKGFRGCNAIKVQLWWAVQATIFAWSPQVLYRWRA
                     FLLRLFGAKIGKNVVIRPSVKITYPWKLTLGDYAWVGDEVNLYTLGEITIGAHSVISQ
                     KSYLCTGSHDHASQHFTINATPIVIGEKCWLATDVFVAPGVTIGDGTVVGARSSVFKS
                     LPANVVCRGNPAVVIRKRVETE"
     gene            9264..10385
                     /gene="gmd"
                     /locus_tag="J1Y83_002857"
     CDS             9264..10385
                     /gene="gmd"
                     /locus_tag="J1Y83_002857"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HBA7630990.1"
                     /translation="MSKVALITGVTGQDGSYLAEFLLEKGYEVHGIKRRASSFNTERV
                     DHIYQDPHTCNPKFHLHYGDLSDTSNLTRILREVQPDEVYNLGAMSHVAVSFESPEYT
                     ADVDAMGTLRLLEAIRFLGLEKKTRFYQASTSELYGLVQEIPQKETTPFYPRSPYAVA
                     KLYAYWITVNYRESYGMYACNGILFNHESPRRGETFVTRKITRAIANIAQGLESCLYL
                     GNMDSLRDWGHAKDYVKMQWMMLQQEQPEDFVIATGVQYSVRQFVEMAAAQLGIKLRF
                     EGTGVEEKGIVVSVTGHDAPGVKPGDVIIAVDPRYFRPAEVETLLGDPTKAHEKLGWK
                     PEITLREMVSEMVANDLEAAKKHSLLKSHGYDVAIALES"
     gene            10388..11353
                     /gene="fcl"
                     /locus_tag="J1Y83_002858"
                     /gene_synonym="wcaG"
                     /gene_synonym="yefB"
     CDS             10388..11353
                     /gene="fcl"
                     /locus_tag="J1Y83_002858"
                     /gene_synonym="wcaG"
                     /gene_synonym="yefB"
                     /EC_number="1.1.1.271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-L-fucose synthase"
                     /protein_id="HBA7630991.1"
                     /translation="MSKQRIFIAGHRGMVGSAIRRQLEQRGDVELVLRTRDELNLLDS
                     RAVHDFFASERIDQVYLAAAKVGGIVANNTYPADFIYQNMMIESNIIHAAHQNDVNKL
                     LFLGSSCIYPKLAKQPMAESELLQGTLEPTNEPYAIAKIAGIKLCESYNRQYGRDYRS
                     VMPTNLYGPHDNFHPSNSHVIPALLRRFHEATAQNAPDVVVWGSGTPMREFLHVDDMA
                     AASIHVMELAHEVWQENTRPMLSHINVGTGVDCTIRELAQTIAKVVGYKGRVVFDASK
                     PDGTPRKLLDVTRLHQLGWYHEISLEAGLASTYQWFLENQDRFRG"
     gene            11356..11835
                     /locus_tag="J1Y83_002859"
     CDS             11356..11835
                     /locus_tag="J1Y83_002859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose mannosyl hydrolase"
                     /protein_id="HBA7630992.1"
                     /translation="MFLRQEDFATVVRSTPLVSLDFIVENSRGEFLLGKRTNRPAQGY
                     WFVPGGRVQKDETLEAAFERLTMAELGLRLPITAGQFYGVWQHFYDDNFSGTEFTTHY
                     VVLGFRIRVAEDELLLPDEQHDDYRWLTPDALLASDNVHANSRAYFLSEKRAGVPGL"
     gene            11832..13055
                     /gene="wcaI"
                     /locus_tag="J1Y83_002860"
     CDS             11832..13055
                     /gene="wcaI"
                     /locus_tag="J1Y83_002860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis fucosyltransferase
                     WcaI"
                     /protein_id="HBA7630993.1"
                     /translation="MKILVYGINYSPELTGIGKYTGEMVEWLAAQGHEVRVITAPPYY
                     PQWQVGENYSAWRYKREEGAATVWRCPLYVPKQPSTLKRLLHLGSFAASSFFPLMAQR
                     RWKPDRIIGVVPTLFCTPGMRLLAKLSGARTVLHIQDYEVDAMLGLGLAGKGKGGKVA
                     QLATAFERSGLHNVDNVSTISRSMMNRAIEKGVAAENVIFFPNWSEIARFQHVADADV
                     DALRNQLGLPDNKKIILYSGNIGEKQGLENVIEAADRLRDEPLIFAIVGQGGGKARLE
                     KMAQQRGLRNMQFFPLQSYDALPALLKMGDCHLVVQKRGAADAVLPSKLTNILAVGGN
                     AVITAEAHTELGQLCETFPGIAVCVEPESVEALVAGIRQALLLPKHNTVAREYAERTL
                     DKENVLRQFINDIRG"
     gene            13058..14494
                     /gene="cpsB"
                     /locus_tag="J1Y83_002861"
     CDS             13058..14494
                     /gene="cpsB"
                     /locus_tag="J1Y83_002861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate guanyltransferase"
                     /protein_id="HBA7630994.1"
                     /translation="MAQSKLYPVVMAGGSGSRLWPLSRVLYPKQFLCLKGDLTMLQTT
                     ICRLNGVECESPVVICNEQHRFIVAEQLRQLNKLTENIILEPAGRNTAPAIALAALAA
                     KRHSPESEPLMLVLAADHVIADEDAFRAAVRNAMPYAEAGKLVTFGIVPDLPETGYGY
                     IRRGEVSAGEQDTVAFEVAQFVEKPNLETAQAYVASGEYYWNSGMFLFRAGRYLEELK
                     KYRPDILDACEKAMSAVDPDLDFIRVDEEAFLACPEESVDYAVMERTADAVVVPMDAG
                     WSDVGSWSSLWEISAHTAEGNVCHGDVINHKTENSYVYAESGLVTTVGVKDLVVVQTK
                     DAVLIADRNAVQDVKKVVEQIKADGRHEHRVHREVYRPWGKYDSIDAGDRYQVKRITV
                     KPGEGLSVQMHHHRAEHWVVVAGTAKVTIDGDIKLLGENESIYIPLGATHCLENPGKI
                     PLDLIEVRSGSYLEEDDVVRFADRYGRV"
     gene            14687..16057
                     /gene="cpsG"
                     /locus_tag="J1Y83_002862"
     CDS             14687..16057
                     /gene="cpsG"
                     /locus_tag="J1Y83_002862"
                     /EC_number="5.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase CpsG"
                     /protein_id="HBA7630995.1"
                     /translation="MKKLTCFKAYDIRGKLGEELNEDIAWRIGRAYGEFLKPKTIVLG
                     GDVRLTSETLKLALAKGLQDAGVDVLDIGMSGTEEIYFATFHLGVDGGIEVTASHNPM
                     DYNGMKLVREGARPISGDTGLRDVQRLAEANDFPPVDETKRGRYQQINLCDAYVDHLF
                     GYINVKNLTPLKLVINSGNGAAGPVVDAIEARFKALGAPVELIKVHNTPDGNFPNGIP
                     NPLLPECRDDTRNAVIKHGADMGIAFDGDFDRCFLFDEKGQFIEGYYIVGLLAEAFLE
                     KNPGAKIIHDPRLSWNTVDVVTAAGGTPVMSKTGHAFIKERMRKEDAIYGGEMSAHHY
                     FRDFAYCDSGMIPWLLVAELVCLKGKTLGELVRDRMSAFPASGEINSKLAHPVEAINR
                     VEQHFSREALAVDRTDGISMTFADWRFNLRSSNTEPVVRLNVESRGDVPLMEARTRTL
                     LTLLNE"
     gene            16113..17507
                     /gene="wcaJ"
                     /locus_tag="J1Y83_002863"
     CDS             16113..17507
                     /gene="wcaJ"
                     /locus_tag="J1Y83_002863"
                     /EC_number="2.7.8.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate glucose
                     phosphotransferase"
                     /protein_id="HBA7630996.1"
                     /translation="MTNLKKRERAKTNASLISMVQRFSDITIMFAGLWLVCEVSGLSF
                     LYMHLLVALITLVVFQMLGGITDFYRSWRGVRAATEFALLLQNWTLSVIFSAGLVAFN
                     NDFDTQLKIWLAWYGLTSIGLVVCRSCIRIGAGWLRNHGYNKRMVAVAGDLAAGQMLM
                     ESFRNQPWLGFEVVGVYHDPKPGGVSNDWAGNLQQLVEDAKAGKIHNVYIAMQMCDGA
                     RVKKLVHQLADTTCSVLLIPDVFTFNILHSRIEEMNGVPVVPLYDTPLSGVNRLIKRA
                     EDIVLATLILLLISPVLCCIALAVKLSSPGPVIFRQTRYGMDGKPIKVWKFRSMKVME
                     NDKVVTQATQNDPRVTKVGNFLRRTSLDELPQFINVLTGGMSIVGPRPHAVAHNEQYR
                     QLIEGYMLRHKVKPGITGWAQINGWRGETDTLEKMEKRVEFDLEYIREWSVWFDIKIV
                     FLTVFKGFVNKAAY"
     gene            17509..18987
                     /gene="wzxC"
                     /locus_tag="J1Y83_002864"
     CDS             17509..18987
                     /gene="wzxC"
                     /locus_tag="J1Y83_002864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M-antigen undecaprenyl disphosphate flippase"
                     /protein_id="HBA7630997.1"
                     /translation="MSLREKTISGAKWSAIATVIIIGLGLVQMTVLARIIDNHQFGLL
                     TVSLVIIALADTLSDFGIANSIIQRKEISHLELTTLYWLNVGLGLVVCVAVFLLSDLI
                     GDLLNNPDLAPLIKTLSLAFVVIPHGQQFRALMQKELEFNKIGMIETSAVLAGFTFTV
                     VSAHFWPLAMTAILGYLVNSAVRTLLFGYFGRKIYRPGLHFSLASVAPNLRFGAWLTA
                     DGIINYLNTNLSTLVLARILGAGVAGGYNLAYNVAVVPPMKLNPIITRVLFPAFAKIQ
                     DDTEKLRINFYKLLSVVGIINFPALLGLMVVSNNFVPLVFGEKWSSIIPVLQLLCIVG
                     LLRSVGNPIGSLLMAKARVDISFKFNVFKTFLFIPAIIIGGQMAGAIGVTLGFLLVQI
                     INTILSYFVMIKPVLGSSYRQYILSLWLPFYLSLPTLVVSYALGIVLKGQLALGMLLA
                     VQIAAGVLAFVVMIVLSRHSLVVEVKRQFCRSEKMKMLLRAG"
BASE COUNT         4583 a         4754 c         5306 g         4452 t
ORIGIN      
        1 tggagagcca atattcaatt accataatgt caccttatat tcggaaatta atttccgcaa
       61 taaccctttc tcatcaacga ctgtatgcag agagggtttg aaaaattaaa ccaaatcagt
      121 gtattggtag ctaaaaagcc aggggcggta gcgtgtctgg atgcctgaga gaccagtctg
      181 aattattctg tcaatagcct gcggatgaaa tcaatttttt taggactgat gccagttaaa
      241 tttttattca attaactgca aggtaaatgt gcatggcgac attgctatta atagtgcaca
      301 ggataattac tctgccaaag tgataaataa acaatgatga aatccaaaat gaaattgatg
      361 ccattattgg tgtcagtaac cttgataagc ggttgcacag tacttccggg cagcaatatg
      421 tcgacgatgg ggaaagacgt catcaaacag caggacgctg atttcgacct cgacaaaatg
      481 gtgaatgttt atccgttaac cccgcgcctg attgatcaat tatgcccacg acccaacgta
      541 gcgcgcccca atatgacgct ggaaagtgag atcgcgaatt accagtatcg cgtcgggccg
      601 ggggacgttc ttaatgtcac cgtctgggat cacccggaac tcaccacgcc agccggtcag
      661 taccgcagct ccagcgacac cggcaactgg gtacagcctg acggcaccat gttctacccg
      721 tatatcggca aggttcacgt cgtggggaaa acgctcgctg aaatccgcag tgatattacc
      781 gggcgcttag cgacgtatat cgctgacccg caggtggacg ttaatatcgc cgccttccgc
      841 tcgcaaaaag cctatatctc cggtcaggtg aataaatccg gtcaacaggc cattaccaac
      901 gtgcctctga ccattctcga cgctatcaac gccgcaggtg gcctgaccga caccgctgac
      961 tggcgcaacg tggtgctaac acacaatggt cgtgaagagc gcatttcttt gcaggcgctg
     1021 atgcaaaacg gcgacctcaa tcagaaccga ctgctttacc ccggcgatat tctttacgtg
     1081 ccacgtaatg atgatctgaa agtgtttgtg atgggtgaag tgaagaaaca gagcaccctg
     1141 aaaatggact ttagcggcat gaccctgact gaagccctgg gcaatgctga aggcatcgac
     1201 atgaccacct ccaacgccag cggcatcttt gtcattcgtc cgctgaaagg cgagagcggg
     1261 cgtaacggca agattgccaa tatctaccag ctggatatgt ccgatgcgac gtcgctggta
     1321 atggcgacag aattccgcct gcaaccttat gacgtggtgt atgtcaccac cgccccggta
     1381 tcccgctgga accgtctgat caatcagttg ctgccaacta ttagcggtgt ccgttacatg
     1441 acggatacag ccagcgacat tcataactgg taatcgtcat gtttaacaac atcttagttg
     1501 tctgtgtcgg caatatttgc cgttccccga cggcggaacg cttactgcaa cgttatcacc
     1561 cggagctaaa agtggagtcc gctggactcg gcgcgctggt cggaaagggc gctgatccta
     1621 ccgctatcag cgtcgccgca gaacatcaac tgtctctgga aggtcattgc gcacggcaaa
     1681 tcagccgccg tctgtgtcgc aactacgacc tgattctgac catggaaaag cgccatatcg
     1741 aacgcttatg cgagatggca cccgagatgc gcggcaaagt gatgctgttt ggtcactggg
     1801 ataacgaatg tgaaatcccc gatccatatc gcaaaagccg ggaaacgttt gcagcggtgt
     1861 acacattact tgaacggtct gcccgccagt gggcgcaggc attgaacgca gagcaggtat
     1921 aagaatgaca gaaaaagtaa aacaacatgc cgctccggta acgggcagtg atgaaatcga
     1981 tattggtcgc ctggtcggca ccgtcattga agcgcgctgg tgggtgattg gcatcaccgc
     2041 cgtatttgcc ctctgtgctg tggtttacac cttctttgcc acgccgattt atagcgccga
     2101 cgcactggta caaatcgagc aaagcagcgg caattcgtta gtgcaggata tcggatcggc
     2161 attagccaac aaaccgcctg catcggacgc cgagatccag ttgattcgtt cgcgcctggt
     2221 gcttggtaaa acggtggacg atctcgacct cgatattgcg gtgagcaaaa acacgttccc
     2281 gattttcggt gcgggctggg agcgcctgat gggacgtcag aacgagacgg tgaaagtgac
     2341 cacctttaac cgaccgaagg agatggcgga tcaggtgttt acgcttaatg tgctggacaa
     2401 caaaaactac accctgagca gcgatggcgg ctttagcgcc cgtgggcaag cgggccagat
     2461 gctgaaaaaa gaaggcgtca cgctgatggt tgaagccatt cacgccagcc cgggcagtga
     2521 gtttaccgtc accaaatact ccacgctggg gatgatcaac caactgcaaa acagcctgac
     2581 ggtaacggag aacggcaaag acgcaggcgt tttgagcctg acttataccg gtgaagatcg
     2641 cgaacagatc cgcgacattc ttaacagcat cgcccgtaac tatcaggaac aaaatattga
     2701 gcgcaaatcg gcggaagcgt cgaaaagcct cgctttcctc gcgcaacagt tacctgaagt
     2761 acgtagccgc cttgatgttg ccgaaaacaa actgaatgcc ttccgtcagg ataaagattc
     2821 agttgatctg ccgctggaag cgaaagcggt gctcgattcg atggtgaaca tcgacgcaca
     2881 attgaacgaa cttaccttta aagaagcgga aatctccaag ctgtacacca aagttcaccc
     2941 ggcgtaccgc acgctgctgg agaaacggca ggcgctggaa gacgaaaaag ccaaacttaa
     3001 tggtcgcgta acggcgatgc cgaaaaccca gcaggaaatt gtgcgtctga cccgcgatgt
     3061 cgagtctggt cagcaggtct atatgcaact gctgaataaa gagcaggagc tgaaaatcac
     3121 cgaagccagc accgtcggcg atgtgcgcat tgttgacccg gcaatcaccc agccaggtgt
     3181 actgaaaccg aagaaagggc tgattatcct tggggcgatt atccttggcc tgatgctctc
     3241 tatcgtgggt gtgctgctgc gctcgttgtt taatcgcggt atcgaaagcc cgcaggtgct
     3301 ggaagaacac ggtatcagcg tttatgccag catcccgctg tcggagtggc agaaagcgcg
     3361 cgatagcgtc aaaaccatca aagggattaa acgctataaa cagagccagc tactggcggt
     3421 ggggaatcca accgatctgg cgattgaagc catccgtagt ctgcgtacca gtctgcactt
     3481 cgcgatgatg caggcacaga acaatgtgtt gatgatgacc ggggttagcc cgtcaatcgg
     3541 taaaaccttt gtctgcgcca acctggcggc ggtgattagc cagaccaata aacgggtgtt
     3601 gttgatcgac tgcgatatgc gcaaaggcta cacccacgag ctgttgggca ctaataacgt
     3661 taatggcctg tcggaaattc tgattggaca gggcgatatt actaccgctg ctaaaccgac
     3721 ctctattgcc aaatttgacc tgatcccgcg cggtcaggta ccgccaaatc cttctgaact
     3781 gttaatgagc gaacgctttg ccgaactggt gaagtgggcg agtaaaaact acgacctggt
     3841 gttgattgat acgccgccga ttctggcagt gaccgatgcg gcaattgttg gtcgtcatgt
     3901 cggaaccacg ttgatggtgg cgcgttatgc agtcaacaca ttgaaagaag tggaaaccag
     3961 tctgagccgc tttgagcaaa acggtattcc ggtgaaaggg gtgattctga actccatctt
     4021 ccgccgcgcc agcgcatatc aggattatgg ctattacgaa tacgaatata agtcggatgc
     4081 gaaataacga cgcctgcatt cgcaccgccc cgtaggccgg ataaggcgtt cacgccgcat
     4141 ccggcaagca aaccagctca taagccggga gaacaaccta tgaaagacaa tccgctgatc
     4201 tcaatctata tgccgacctg gaatcgccaa caactggcga ttcgggcgat aaaatcggtc
     4261 ctgcgccagg actataacaa ctgggagatg atcatcgtgg atgattgctc cacctcctgg
     4321 gaacaactcc aacagtacgt caccgccctc aacgatccgc gtattactta cattcacaac
     4381 gacattaaca gcggggcgtg cgcggtacgt aaccaggcga ttatgctggc gcaaggggaa
     4441 tacatcaccg ggatcgatga cgacgatgaa tggataccca accgcctgag cgtcttcctc
     4501 gcccataaac agcaactgat cacacacgcc tttttgtacg ctaacgacta cgtttgccag
     4561 ggcgaagttt attcccaacc ggcaagcttg ccgctgtatc ccaaatcgcc gtattcacgc
     4621 cgcctgttct acaaacgcaa tatcattggc aatcaggtct ttacctgggc gtggcgtttc
     4681 aaagagtgct tgttcgatac cgaactgaaa gcggcacagg attacgacat cttcctgcgg
     4741 atggtggtgg agtacggcga accgtggaaa gtggaagagg cgacgcagat cctgcatatc
     4801 aatcacggcg aaatgcagat cacctcgtca ccgaaaaaat tctccggata cttccacttt
     4861 taccgcaagc acaaagacaa attcgaccgc gccagcaaaa aatatcagct gtttaccctc
     4921 taccagatcc gcaataaacg catgacctgg cgcactttgc tgacgctgct gtcggtgcgt
     4981 aacggcaaac gtctggctga tggtattcgg gggcgctaaa gatgctggaa gatctgcgcg
     5041 ccaacagctg gagtttacgc ccgtgctgca tggttctcgc ctatcgcgct gctcattttt
     5101 gttcggtgtg gcgcaaaaag aacgtcctca acaatctttg ggcggccccg ctgctggtgc
     5161 tgtatcgcat tatcaccgaa tgctttttcg gttatgaaat ccaggccgcc gcgaccattg
     5221 gccgacgctt tactatccat cacggttacg ccgtagtgat caataaaaac gtggtagcgg
     5281 gggatgattt caccattcgt cacggcgtca ctatcggcaa tcgtggtgcc gataacatgg
     5341 catgtccaca cattggcaac ggcgtcgaac tcggtgccaa cgtcattatt cttggtgata
     5401 tcacgcttgg taacaacgtc accgtgggcg cgggcagcgt ggtgctcgac tctgtcccgg
     5461 acaacgcgct ggtggtggga gaaaaagcgc gagtgaaggt aattaaatga atattctgca
     5521 atttaatgtg cgactggcgg aaggcggggc agcaggtgtg gcgttagatc tccaccagcg
     5581 cgcgctgcaa caggggctgg cgtcacattt tgtctacggc tacggcaaag gcggcaaaga
     5641 gagcgtcagc catcaaaact atccgcaggt catcaaacat acgccgcgga tgaccgcgat
     5701 ggcgaacatt gcgttgtttc gtctgcttaa tcgcgatctg ttcggcaatt tcaatgagtt
     5761 atatcgcacc attactcgca caccgggccc ggtggttctg cattttcatg tgctgcacag
     5821 ctactggcta aatcttaaga gcgtggtgcg cttttgcgaa aaagtgaaaa accacaaacc
     5881 ggatgtcact ctggtctgga cgttacacga tcactggagc gttaccggac gctgcgcctt
     5941 taccgacggt tgcgaaggct ggaaaacggg ctgccagaaa tgcccgacct taaataacta
     6001 tccaccggtg aagattgatc gcgcgcacca gctggtggcg ggtaaacgcc agttattccg
     6061 cgagatgctg gcactgggct gtcagtttat ttcccccagc cagcatgtgg ctgatgcttt
     6121 caatagtctg tacggtccag ggcgttgccg gattatcaat aacggtattg atatggcaac
     6181 cgaagcgatt ctggcggact tgcctccggt gcgcgaaacc caggacaagc cgaaaatcgc
     6241 ggtggtggcg catgatctgc gttacgacgg caaaactaac cagcaactgg tacgtgagat
     6301 gatggcgctg ggcgacaaaa ttgaactgca tacctttggt aagttctcgc cgttcaccgc
     6361 tggcaacgtg gttaatcacg gctttgaaac tgacaagcgc aagttgatga gcgcgctcaa
     6421 tcagatggat gcgctggtat tcagttctcg cgtcgataac tacccactga ttttgtgtga
     6481 ggcgctatcg attggtgtgc cggtgattgc cacccatagc gatgcggcgc gggaagtgct
     6541 gcaaaaatcc ggcggtaaaa ccgtcagcga agaagacgtg ctgcaactga tccagttaag
     6601 caaaccggaa atcgcgcagg cgatatttgg taccacgctg gctgagttca gccaacgcag
     6661 ccgcgccgcc tacagtggac aacagatgct ggaggagtat gtcaacttct atcagaatct
     6721 gtagctacct gctgctgccg ctgatttacc tgttggttaa cgtcaaaatc gcccagcttg
     6781 gcgaaagttt ccccatcacc atcgtcactt ttttacctgt cttgttactg ctgtttttag
     6841 aacgcatcag cgttaaaaaa ttgatgattg ccttagggat tggcgcgggg ctcacggcgt
     6901 ttaactacct gtttggtcag tcgttggatg ccagtaaata cgtcacttca accatgctgt
     6961 ttgtctatat tgtgatcatt attggcatgg tgtggagtat tcgctttaaa acgatttcgc
     7021 cacacaacca tcgcaagata ttacgtttct tttatctggt ggtcgggctg gtggtggcgc
     7081 tggcggcagt ggagatggcg caaattatcc tcaccggtgg cagcagtatt atggagtcga
     7141 tttcgaaata tctgatttac agcaacagct atgtgctgaa tttcatcaaa ttcggcggca
     7201 agcgcacgac agcactttat ttcgaaccgg catttttcgc tctggcatta atctcaattt
     7261 ggctcagcat caaacagttt ggtatcaaaa cgcctaaaac cgatgctatg attctcgcag
     7321 ggataatatt atccggatcg ttttcagggg ttatgacctt tatcctgttt tatttgctgg
     7381 agtgggcatt tcaatatctg aataaagagg cgattaagaa aaagttaccg ttagcattga
     7441 tttctctggc tgtattcctg gttggtgtgg taatcgcgtt tccttatatt tccacccgtc
     7501 tgggtgattt aggcacggaa ggttcgtcat cgtattatcg tattgtcggt ccgctggtga
     7561 tggtcggtta ttctttgacc catattgacg gtgtagtcag atttggctca ctttatgaat
     7621 atgtcgcatc attcggaata tttaacggtg cggatgtcgg aaaaaccata gacaatggtt
     7681 tgtatctgct gattatttat ttttcctggt tcgcggtgtt tttatcactg tggtacatgg
     7741 ggaaagtgat aaaaatgatg atcaacgctt ttggtgataa ccgcaatttt cgcgtgcaat
     7801 tatatctttt tactccggta tcgctgtttt ttaccggttc gatatttagc ccggaatatg
     7861 catttttaat cgtctgtccg tttattttgc gaaaagcgtt aaatattacg aggtaagaat
     7921 aagaacatgt tgcttagcat aatcactgtc gcgtttcgta acctcgaagg gatagtcaaa
     7981 acacatgcct cgctggcgca tctggcgcag gcggacgata tcagcttcga atggattgtt
     8041 gtcgatggcg gttccaatga cggcacccgt gagtatctgg aaaatctcaa tggtatctat
     8101 aacctacgct ttgtcagcga gccagataac ggtatctacg acgctatgaa caaaggcatt
     8161 gcgatggcgc aaggcaagtt cgcgttgttt ctcaactcag gcgatatttt tcatcaggat
     8221 gccgcaaatt ttgtccgtaa gttaaaaatg caaaaagata acatgatgat caccggcgat
     8281 gcgctgctgg attttggcga cgggcataaa attaaacgta acgccaaacc gggttggtat
     8341 atttaccaca gcctgcccgc cagtcatcag gcgatatttt tcccggtgtc cggcttgaag
     8401 aaatggcgtt atgacctgga atataaagtt tcttccgatt acgcgctggc ggccaaaatg
     8461 tataaagccg gttatgcatt taaaaaactc aatggcctgg tgtctgaatt ttctatgggt
     8521 ggggtatcta ccaccaataa tatggaattg tgtgctgacg ccaaaaaagt ccaacggcag
     8581 atattgcacg tgcctggctt ttgggctgaa ttatcctggc atttacgcca acgtacgacc
     8641 tcaaagacga aagccttata taacaaaagc tgaatataag ggaaaacgat gcaggattta
     8701 agtggattct cggtgccgaa agggttccgg ggttgcaacg ctattaaagt gcaattgtgg
     8761 tgggcagtac aggcaacaat atttgcctgg tcgccacaag tattgtatcg ctggcgggca
     8821 tttttattac gtttattcgg cgcaaaaata ggaaaaaacg tagttattcg tccgtcagta
     8881 aaaattacct atccgtggaa attaacgtta ggtgattacg cgtgggtcgg cgatgaggtc
     8941 aatttatata ccctcggtga aataaccatt ggcgcacatt cggtgatatc gcaaaaaagt
     9001 tatttatgca ctggtagcca cgaccatgca agtcaacatt tcaccattaa cgccacgcct
     9061 attgtgattg gcgagaaatg ctggctggca accgatgtct ttgttgcccc tggcgtcacg
     9121 atcggcgacg gcaccgtcgt gggtgcgcga agcagtgttt ttaaatcgct tccggcaaat
     9181 gtggtttgtc gggggaaccc cgcagtggtg atacgcaaac gcgttgaaac tgaataaatt
     9241 caaaaaatac agaggaataa gacatgtcaa aagtcgctct catcaccggt gtaaccggac
     9301 aagacggttc ttacctggca gagtttctgc tggaaaaagg ttacgaggtg catggtatta
     9361 agcgtcgtgc atcgtcattc aacaccgagc gcgtggatca tatttatcag gatccgcaca
     9421 cctgcaaccc gaaattccat ctgcattatg gcgacctgag tgatacctcc aacctgacgc
     9481 gcattttgcg tgaagtgcag ccggatgaag tgtacaacct gggcgcaatg agccacgttg
     9541 cggtttcttt tgagtcaccg gaatataccg cagacgtcga tgcgatgggt acgctgcgcc
     9601 tgctggaggc gatccgcttc cttggtctgg aaaagaaaac ccgtttctat caggcttcca
     9661 cttctgaact gtacggtctg gtgcaggaaa ttccgcagaa agagaccacg ccgttctacc
     9721 cgcgatctcc gtatgcggtc gccaaactgt acgcctactg gatcaccgtt aactaccgcg
     9781 aatcctacgg catgtacgcc tgtaacggta ttctcttcaa ccatgaatcc ccgcgccgcg
     9841 gcgaaacctt cgttacccgc aaaatcaccc gcgcaatcgc caacatcgcc caggggctgg
     9901 aatcgtgcct gtacctcggc aatatggatt ccctgcgtga ctggggccat gccaaagact
     9961 acgtaaaaat gcagtggatg atgctgcaac aggaacaacc ggaagatttc gttattgcca
    10021 ccggcgttca gtactccgtg cgtcagttcg tggaaatggc ggcagcacaa ctgggcatca
    10081 aactgcgctt tgaaggtacg ggcgttgaag agaagggcat tgtggtttcc gtcaccgggc
    10141 atgacgcgcc gggcgttaaa ccgggcgatg taattatcgc cgttgacccg cgttacttcc
    10201 gtccggctga agttgaaacg ctgctcggcg acccgaccaa agcacacgaa aagctgggct
    10261 ggaaaccgga aatcaccctc agagaaatgg tatctgaaat ggtggctaat gacctcgaag
    10321 cggcgaaaaa acactctctg ctgaaatctc acggctacga cgtggcgatc gcgctggagt
    10381 cataagcatg agtaaacaac gcatttttat cgctggccat cgcgggatgg tcggttctgc
    10441 catcaggcgg cagctcgaac agcgcggtga tgtggaactg gtattacgca cccgcgacga
    10501 actgaacctg ctggacagcc gcgcggtgca tgatttcttt gccagcgagc gcattgacca
    10561 ggtctatctg gcggcggcga aagtaggcgg cattgttgcc aacaacacat atccggcaga
    10621 tttcatctac cagaacatga tgattgagag caacatcatt cacgccgcgc atcagaacga
    10681 cgtgaacaaa ctgctgtttc tcggatcgtc ctgcatctac ccgaaactgg caaaacagcc
    10741 gatggcagaa agcgagttgt tgcagggcac gctggagccg accaacgagc cttacgccat
    10801 tgccaaaatc gccgggatca aactgtgtga atcttacaac cgccagtacg gacgggatta
    10861 ccgctcagtc atgccaacca acctgtacgg gccgcacgac aacttccacc cgagtaactc
    10921 gcacgtgatc cctgcattgc tgcgtcgctt ccacgaggcg acggcacaga atgcgccgga
    10981 cgtggtggtg tggggcagcg gtacgccgat gcgcgagttt ctgcacgttg atgatatggc
    11041 agcggcgagc attcatgtga tggagctggc gcacgaagtc tggcaggaga atacccggcc
    11101 gatgctgtcg cacattaacg tcggcacggg cgttgactgc actatccgcg agctggcgca
    11161 aaccatcgcc aaagtggtgg gttacaaagg tcgggtagtt tttgatgcca gcaaaccgga
    11221 tggcacgccg cgcaaattgc tggatgtgac gcggctgcat cagcttggct ggtatcacga
    11281 aatctcactg gaagcggggc ttgccagcac ttaccagtgg ttccttgaga atcaagaccg
    11341 ctttcggggg taatgatgtt tttacgtcag gaagactttg ccacggtagt gcgctccaca
    11401 ccgcttgtct ctctcgactt tattgttgag aacagtcgcg gcgagtttct gcttggcaaa
    11461 agaaccaacc gcccggcgca gggttactgg tttgtgccgg gagggcgcgt gcagaaagac
    11521 gaaacgctgg aagccgcatt tgagcggctg acgatggcgg aactggggct gcgtctgccg
    11581 ataacagcag gccagtttta cggtgtctgg cagcactttt atgacgataa cttctccggc
    11641 acggaattca ccactcacta tgtggtgctc ggttttcgca tcagagtcgc ggaagatgag
    11701 ctgttactgc cggatgagca gcatgacgat taccgctggc tgacgccgga cgcgttgctc
    11761 gccagtgaca atgttcacgc taacagccgc gcctattttc tctctgagaa gcgtgccgga
    11821 gtacccggat tatgaaaata ctggtctacg gcattaacta ctcgccggag ttaaccggca
    11881 tcggcaagta caccggcgag atggtggaat ggctggcagc acaaggtcat gaggtgcggg
    11941 ttattaccgc gccgccttac tacccgcagt ggcaggtggg cgagaactat tccgcctggc
    12001 ggtacaaacg ggaagagggg gccgccacgg tgtggcgctg cccgctgtac gtgccaaaac
    12061 agccgagcac cctgaaacgc ctgttgcatc tcggcagttt tgccgccagc agtttctttc
    12121 cgctgatggc gcaacgtcgc tggaagccgg atcgcattat cggcgtggtg ccaacgctgt
    12181 tttgcacgcc aggaatgcgc ctgctggcga aactgtctgg tgcgcgcacg gtgctccata
    12241 ttcaggatta cgaagtggac gccatgctgg ggctgggcct tgccggaaaa ggtaaaggcg
    12301 gcaaagtggc acagctggca acggcgttcg aacgtagcgg actgcataac gtcgataacg
    12361 tctccacgat ttcgcgttcg atgatgaata gagctatcga gaaaggcgtg gcggcggaaa
    12421 acgtcatctt cttccccaac tggtcagaaa ttgcccgttt tcaacatgtt gcagacgccg
    12481 atgttgacgc ccttcgtaac cagcttggcc tgccggataa caaaaaaatc attctttact
    12541 ccggcaatat tggtgaaaag caggggctgg aaaacgttat tgaagctgcc gatcgcctgc
    12601 gcgatgaacc gcttattttt gccattgtcg ggcagggcgg cggcaaagcg cggctggaaa
    12661 aaatggcgca gcagcgtgga ctgcgcaaca tgcaattttt tccgctgcaa tcgtatgacg
    12721 ctttacccgc actgctgaag atgggggatt gccatctggt ggtgcaaaaa cgcggcgcgg
    12781 cagatgcagt attgccgtcg aaactgacca atattctggc ggtaggcggt aacgcggtga
    12841 ttaccgcaga agcccacacc gaactggggc agctttgcga aacctttccg ggcattgcgg
    12901 tttgcgtgga accggaatcg gtcgaggcgc tggtggcggg gattcgtcag gcgctcctgc
    12961 tgcccaaaca caacacggtg gcacgtgaat atgccgaacg cacgctcgat aaagagaacg
    13021 tgttacgtca atttataaat gatattcggg gataattatg gcgcagtcga aactctatcc
    13081 agttgtgatg gcaggtggct ccggtagccg cttatggccg ctttcccgcg tactttaccc
    13141 caagcagttt ttatgcctga aaggcgatct caccatgctg caaaccacca tctgccgcct
    13201 gaacggcgtg gagtgcgaaa gcccggtggt gatttgcaat gagcagcacc gctttattgt
    13261 cgcggaacag ctgcgtcaac tgaacaaact caccgagaac attattctcg aaccggcagg
    13321 gcgtaacacc gcacctgcca ttgcgctggc ggcgctggca gcaaaacgtc atagcccgga
    13381 gagcgaaccg ttaatgttgg tgctggcggc agatcatgtg attgccgacg aagatgcgtt
    13441 ccgcgccgcc gtgcgtaacg ccatgccgta tgctgaagcg ggcaagctgg tgaccttcgg
    13501 cattgtgccg gatctaccag aaaccggtta tggctatatt cgtcgcggtg aagtgtcggc
    13561 gggtgagcag gatacggtgg cctttgaagt ggcgcagttt gtcgaaaaac cgaatctgga
    13621 aaccgcgcag gcctatgtgg caagtggcga atattactgg aacagcggta tgttcctgtt
    13681 ccgtgccgga cgttatctcg aagaactgaa aaagtatcgt ccggatattc tcgatgcctg
    13741 tgaaaaagcg atgagcgccg tcgatccgga tctcgatttt attcgtgtgg atgaagaggc
    13801 gtttctcgcc tgtccggaag agtcggtaga ttacgcggtc atggaacgca cggcagatgc
    13861 cgttgtggtg ccgatggatg cgggctggag cgatgtcggt tcctggtcct ctttatggga
    13921 gatcagtgcc cacaccgccg agggcaacgt ttgccatggc gatgtgatta atcacaaaac
    13981 tgaaaacagc tatgtgtacg ccgaatctgg cctggtcacc accgtcgggg tgaaagattt
    14041 ggtggtagtg cagaccaaag atgcagtgct gattgccgac cgtaacgcgg tgcaggatgt
    14101 gaaaaaagtg gtcgagcaga tcaaagctga tggtcgccat gagcatcggg tgcatcgcga
    14161 agtgtatcgt ccgtggggca aatatgactc tatcgacgcg ggcgaccgct accaggtgaa
    14221 acgcatcacc gtgaaaccgg gcgaaggctt gtcggtacag atgcatcatc atcgcgcgga
    14281 acactgggtg gttgtcgcgg gaacggcaaa agtcactatc gacggtgata tcaaactgct
    14341 tggtgaaaac gagtccattt atattccgct gggggcgacg cattgcctgg aaaacccggg
    14401 gaaaattccg ctcgatttaa ttgaagtgcg ctccggctct tacctcgaag aggatgatgt
    14461 ggtgcgcttc gcggatcgct acggacgggt gtaaacgtcg catcaggcgc tgtttgtcgg
    14521 atgcggtgtg aacgccttat ccgacctacg gttcgggttt gtaggcctga taagacgcgg
    14581 cagcgtcgca tcaggcaatg aatgcgaaac cgcgatgtaa ataacgacaa aaacaaaatt
    14641 ggccgtttcg gtcagggcca actattgcct gaaaaagggt aacgatatga aaaaattaac
    14701 ctgctttaaa gcctatgata ttcgcggaaa attaggcgaa gaactgaatg aagatattgc
    14761 ctggcgcatt ggtcgcgcct atggcgaatt tctcaaaccg aaaaccattg tgttaggcgg
    14821 tgatgtccgc ctcaccagcg aaaccttaaa actggcgctg gcgaaaggtt tacaggatgc
    14881 gggcgtcgac gtgctggata ttggtatgtc cggcaccgaa gaaatctatt tcgccacgtt
    14941 ccatctcggc gtggatggcg gcatcgaagt taccgccagc cataacccga tggattacaa
    15001 cggcatgaag ctggtgcgcg aaggggctcg cccgatcagc ggcgataccg gactgcgcga
    15061 cgtccagcgt ctggcagaag ccaacgactt tcctcccgtc gatgaaacca aacgcggtcg
    15121 ctatcagcaa atcaatctgt gtgacgctta cgttgatcac ctgttcggtt atatcaacgt
    15181 caaaaacctt acgccgctca agctggtgat taactccggg aacggcgcgg cgggtccggt
    15241 ggtggacgcc attgaagccc gctttaaagc cctcggcgca ccggtggaat tgatcaaagt
    15301 gcataacacg ccggacggca atttccccaa cggtattcct aacccgttgc tgccggaatg
    15361 tcgcgacgat acccgcaatg cggtcattaa acacggcgcg gatatgggca ttgcctttga
    15421 tggcgatttt gaccgctgtt tcctgtttga cgaaaaaggg cagtttattg agggctacta
    15481 cattgtcggc ctgctggcag aagcgttcct cgaaaaaaat cccggcgcga agatcatcca
    15541 cgatccacgt ctctcctgga acaccgttga tgtggtgact gccgcaggcg gcaccccggt
    15601 aatgtcgaaa accggacacg cctttattaa agaacgtatg cgcaaggaag acgccatcta
    15661 cggtggcgaa atgagcgccc accattactt ccgtgatttc gcttactgcg acagcggcat
    15721 gatcccgtgg ctgctggtcg ccgaactggt gtgcctgaaa ggaaaaacgc tgggcgaact
    15781 ggtgcgcgac cggatgtcag cgtttccggc aagcggtgag atcaacagca aactggcaca
    15841 ccccgttgag gcgattaacc gcgtggaaca gcactttagc cgcgaggcac tggcggtgga
    15901 tcgtaccgat ggcatcagca tgacctttgc cgactggcgc tttaatctgc gctcctccaa
    15961 caccgaaccg gtggtgcggt tgaatgtgga atcacgcggt gatgtgccgc tgatggaagc
    16021 gcgaacgcga actctgctga cgttgctgaa cgagtaatgt cggatcttcc cttaccccca
    16081 ctgcgggtaa ggggctaaaa acaggaacaa cgatgacaaa tctaaaaaag cgcgagcgag
    16141 cgaaaaccaa tgcatcgtta atctctatgg tgcaacgctt ttcagatatc accatcatgt
    16201 ttgccgggct atggctggtt tgcgaagtga gcggactgtc attcctctac atgcacctgt
    16261 tggtggcgct gattacgctg gtggtgttcc agatgctggg cggcatcacc gatttttatc
    16321 gctcatggcg cggtgttcgg gcagcgacag aatttgccct gttgctacaa aactggacgt
    16381 taagcgtgat tttcagcgcc ggactggtgg cgttcaacaa tgatttcgac acgcaattga
    16441 aaatctggct ggcgtggtat gggctgacca gcatcggact ggtggtttgc cgttcatgta
    16501 ttcgtattgg ggcgggctgg ctgcgtaatc atggctataa caagcgcatg gtcgcggtgg
    16561 cgggggattt agccgccgga caaatgctga tggagagctt ccgtaatcag ccgtggttag
    16621 ggtttgaagt ggtgggcgtt taccacgacc cgaaaccggg tggcgtttct aacgactggg
    16681 cgggtaatct gcaacagctg gtcgaggatg cgaaagcggg caagattcat aacgtctata
    16741 tcgcgatgca gatgtgcgac ggcgcgcgag tgaaaaaact ggtccatcaa ctggcggaca
    16801 ccacctgttc ggtgctgctg atccccgatg tctttacctt caacattctc cattcccgta
    16861 tcgaagagat gaacggcgta ccggtggtgc cgctgtatga cacgccgctt tccggggtta
    16921 accgcctgat aaaacgtgcg gaagatattg tgctggcgac gcttatcctg ctgctgatct
    16981 ccccggtgct gtgctgcatt gcgctggcgg tgaaactgag ctcaccgggg ccggttattt
    17041 tccgccagac tcgctacggc atggatggca agccgatcaa agtgtggaag ttccgttcta
    17101 tgaaagtgat ggagaacgac aaagtggtga ctcaggcgac gcagaacgat ccgcgcgtta
    17161 ctaaagtggg gaactttctg cgccgcactt cgctggatga actgccgcag tttatcaatg
    17221 tgctgactgg cgggatgtcg attgttggtc cccgtccgca tgcggtggcg cataacgaac
    17281 agtatcgcca gctcattgaa ggctacatgc tgcgccataa agtgaaaccg ggcattaccg
    17341 gctgggcgca gattaacggc tggcgcggcg aaaccgacac gctggagaaa atggaaaaac
    17401 gcgtcgagtt cgaccttgag tacattcgcg aatggagcgt ctggttcgat atcaaaatcg
    17461 ttttcctgac ggtattcaaa ggtttcgtta acaaagcggc atattgatat gagcttacgt
    17521 gaaaaaacca tcagcggcgc gaagtggtcg gcgattgcca cggtgatcat catcggcctc
    17581 ggtctggtgc aaatgactgt gctggcacgg attatagata accaccagtt cggtttgctg
    17641 actgtgtcgc tggtgatcat cgcgctggca gatacgcttt ctgacttcgg tatcgctaac
    17701 tcgattattc agcgaaaaga aatcagccac cttgaactca ctacgctgta ctggctgaac
    17761 gtcgggctgg ggcttgtggt gtgcgtggcg gtgtttttgt tgagtgatct catcggcgac
    17821 ttgctgaata acccggacct ggcaccgttg attaaaacat tgtcgctggc gtttgtggtg
    17881 atcccccacg ggcaacagtt ccgcgcgttg atgcaaaagg agctggagtt caacaaaatc
    17941 ggcatgatcg aaaccagcgc ggtgctggcg ggctttactt ttacggtggt tagcgcccat
    18001 ttctggccgc tggcgatgac cgcgattctc ggttatctgg tcaatagtgc ggtgagaacg
    18061 ctgctgtttg gctactttgg ccgcaaaatt tatcgccccg gtctgcattt ctcgctggcg
    18121 tcggtggcac cgaacttacg ctttggtgcc tggctgacgg cggacggcat catcaactat
    18181 ctcaatacca acctttcaac gctggtgctg gcgcgtattc tcggcgcggg cgtggctggg
    18241 ggatacaacc tggcgtacaa cgtggccgtt gtgccaccga tgaagctgaa cccgatcatc
    18301 acccgcgtgt tgtttccggc gttcgccaaa attcaggacg atacagaaaa gctgcgcatc
    18361 aacttctaca agctgctgtc ggtggtgggg attatcaact ttccggcgct gctcgggctg
    18421 atggtggtgt cgaataactt tgtaccgctg gtatttggcg aaaagtggag cagcattatt
    18481 ccggtgctgc aattgctgtg tattgtgggg ctactgcgct ccgtgggtaa cccgattggt
    18541 tcgctgctga tggcgaaagc gcgggtcgat atcagcttta aattcaacgt attcaaaacc
    18601 tttctgttta ttccggcaat catcattggt gggcagatgg cgggcgcaat cggcgtcacg
    18661 cttggcttcc tgctggtgca gattatcaac accattctga gctatttcgt gatgattaaa
    18721 ccggtgctcg gttccagtta tcgccagtac atcctgagtt tgtggctgcc gttttatctc
    18781 tcgctgccga cgctggtggt cagttatgcg ctgggcattg tgctgaaagg gcaactggcg
    18841 ctggggatgc tgctggcggt gcaaatagcc gcaggcgtgc tggcatttgt ggtgatgatt
    18901 gtgctgtcgc gccattcgct ggtggtggaa gtgaagcgtc agttttgtcg cagcgaaaaa
    18961 atgaaaatgc ttttacgggc ggggtgaatg gttgttttcg gttccggcaa cggttgccgg
    19021 atgcggcgta aaacgcctta tccgtcctgg ggggctgggc ttggatcttg taggtctgat
    19081 aagacgcgtt agcgt
//
LOCUS       DADSYM010000096        18824 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2415
            SAMN08039579-rid11723173.denovo.096, whole genome shotgun sequence.
ACCESSION   DADSYM010000096 DADSYM010000000
VERSION     DADSYM010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039579
            Sequence Read Archive: SRR6362533
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18824)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18824)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980162.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 03:39:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,484
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 4,347
            CDSs (with protein)               :: 4,347
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 43 of 85
            Pseudo Genes (incomplete)         :: 37 of 85
            Pseudo Genes (internal stop)      :: 19 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18824
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039579-rid11723173.denovo.096"
                     /strain="LGE2415"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8302 N 95.9628 W"
                     /collection_date="2015-08-24"
     gene            192..1763
                     /gene="garD"
                     /locus_tag="J1Y83_002865"
     CDS             192..1763
                     /gene="garD"
                     /locus_tag="J1Y83_002865"
                     /EC_number="4.2.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate dehydratase"
                     /protein_id="HBA7630998.1"
                     /translation="MANIKIRQETPTAFYIKVHDTDNVAIIVNDNGLKAGTRFPDGLE
                     LIEHIPQGHKVALLDIPANGEIIRYGEVIGYAVRAIPRGSWIDESMVVLPEAPPLHTL
                     PLATKVPEPLPPLEGYTFEGYRNADGSVGTKNLLGITTSVHCVAGVVDYVVKIIERDL
                     LPKYPNVDGVVGLNHLYGCGVAINAPAAVVPIRTIHNISLNPNFGGEVMVIGLGCEKL
                     QPERLLTGTDDVQAIPVESASIVSLQDEKHVGFQSMVEDILQVAECHLQKLNQRQRET
                     CPASELVVGMQCGGSDAFSGVTANPAVGYASDLLVRCGATVMFSEVTEVRDAIHLLTP
                     RAVNEEVGKRLLEEMEWYDNYLNMGKTDRSANPSPGNKKGGLANVVEKALGSIAKSGK
                     SAIVEVLSPGQRPTKRGLIYAATPASDFVCGTQQVASGITVQVFTTGRGTPYGLMAVP
                     VIKMATRTELANRWFDLMDINAGTIATGEETIEEVGWKLFHFILDVASGKKKTFSDQW
                     GLHNQLAVFNPAPVT"
     gene            1912..2247
                     /gene="prlF"
                     /locus_tag="J1Y83_002866"
     CDS             1912..2247
                     /gene="prlF"
                     /locus_tag="J1Y83_002866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin PrlF"
                     /protein_id="HBA7630999.1"
                     /translation="MPANARSNAVLTTESKVTIRGQTTIPAPVREALKLKPGLDSIHY
                     EILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNNPQKTRPFDIQQGKKLIAGMDVN
                     IDDEIGDDE"
     gene            2247..2711
                     /locus_tag="J1Y83_002867"
     CDS             2247..2711
                     /locus_tag="J1Y83_002867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system YhaV family
                     toxin"
                     /protein_id="HBA7631000.1"
                     /translation="MDFPQRVNGWALYAHPCFQETYDALVAEVEALKGKDPENYQRKA
                     ATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKNKDWSRVKFGAGRYRLFFRYSEK
                     EKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWESLTQETEENH"
     gene            complement(2766..3575)
                     /gene="agaR"
                     /locus_tag="J1Y83_002868"
     CDS             complement(2766..3575)
                     /gene="agaR"
                     /locus_tag="J1Y83_002868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aga operon transcriptional regulator AgaR"
                     /protein_id="HBA7631001.1"
                     /translation="MSNTDASGEKRVTGTSERREQIIQRLRQQGSVQVNDLSALYGVS
                     TVTIRNDLAFLEKQGIAVRAYGGALICDSTTPSVEPSVEDKSALNTAMKRSVAKAAVE
                     LIQPGHRVILDSGTTTFEIARLMRKHTDVIAMTNGMNVANALLEAEGVELLMTGGHLR
                     RQSQSFYGDQAEQSLQNYHFDMLFLGVDAIDLERGVSTHNEDEARLNRRMCEVAERII
                     VVTDSSKFNRSSLHKIIDTQRIDMIIVDEGIPADSLEGLRKAGVEVILVGE"
     gene            3824..5104
                     /gene="kbaZ"
                     /locus_tag="J1Y83_002869"
     CDS             3824..5104
                     /gene="kbaZ"
                     /locus_tag="J1Y83_002869"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit KbaZ"
                     /protein_id="HBA7631002.1"
                     /translation="MKHLTEMVRQHKAGKTNGIYAVCSAHPLVLEAAIRYASANQTPL
                     LIEATSNQVDQFGGYTGMTPADFRGFVCQLADSLNFPQDALILGGDHLGPNRWQNLPA
                     AQAMANADDLIKSYVAAGFKKIHLDCSMSCQDDPIPLTDGIVAERAARLAKVAEETCL
                     EHFGEADLEYVIGTEVPVPGGAHETLSELAVTTPDAARATLEAHRHAFEKQGLNAIWP
                     RIIALVVQPGVEFDHTNVIDYQSAKATALSQMVENYETLIFEAHSTDYQTPQSLRQLV
                     IDHFAILKVGPALTFALREALFSLAAIEEELVPAKACSGLRQVLENVMLDRPEYWQSH
                     YHGDGNARRLARGYSYSDRVRYYWPDSQIDDAFAHLVRNLADSPIPLPLISQYLPLQY
                     VKVRSGELQPTPRELIINHIQDILAQYHTACEGQ"
     gene            5127..5600
                     /gene="agaV"
                     /locus_tag="J1Y83_002870"
     CDS             5127..5600
                     /gene="agaV"
                     /locus_tag="J1Y83_002870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312038.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IIB"
                     /protein_id="HBA7631003.1"
                     /translation="MPNIVLSRIDERLIHGQVGVQWVGFAGANLVLVANDEVAEDLVQ
                     QNLMEMVLAEGIAVRFWTLQKVIDNIHRAADRQKILLVCKTPADFLTLVKGGVPVNRI
                     NVGNMHYANGKQQIAKTVSVDAGDIAAFNDLKAAGVECFVQGVPTEPAVDLFKLL"
     gene            5611..6390
                     /locus_tag="J1Y83_002871"
     CDS             5611..6390
                     /locus_tag="J1Y83_002871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HBA7631004.1"
                     /translation="MEISLLQAFALGIIAFIAGLDMFNGLTHMHRPVVLGPLVGLVLG
                     DLHTGILTGGTLELVWMGLAPLAGAQPPNVIIGTIVGTAFAITTGVKPDVAVGVAVPF
                     AVAVQMGITFLFSVMSGVMSRCDRMAENADTRGIERVNYLALLALGTFYFLCAFLPIY
                     FGAEHAKTIIDVLPQRLIDGLGVAGGIMPAIGFAVLLKIMMKNVYIPYFILGFVAAAW
                     LKLPVLAIAAAALAMALIDLLRKSPEPTQPAAQKEEFEDGI"
     gene            6380..7258
                     /locus_tag="J1Y83_002872"
     CDS             6380..7258
                     /locus_tag="J1Y83_002872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019079236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HBA7631005.1"
                     /translation="MASNQTTLPNVSENEETLLTGVNENVYEDQSIGAELTKKDINRV
                     AWRSMLLQASFNYERMQASGWLYGLLPALKKIHTNKRDLARAMKGHMGFFNTHPFLVT
                     FVIGIILAMERSKQDVNSIQSTKIAVGAPLGGIGDAMFWLTLLPICGGIGASLALQGS
                     ILGAVVFIVLFNVVHLGLRFGLAHYAYRMGVAAIPLIKANTKKVGHAASIVGMTVIGA
                     LVATYVRLSTTLEITAGDAVVKLQADVIDKLMPAFLPLVYTLTMFWLVRRGWSPLRLI
                     AVTVVLGIVGKFCHFL"
     gene            7276..7710
                     /locus_tag="J1Y83_002873"
     CDS             7276..7710
                     /locus_tag="J1Y83_002873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBA7631006.1"
                     /translation="MLSIILTGHGGFASGMEKAMKQILGEQSQFIAIDFPETSSTAQL
                     TSQLEEAIAQLDCEDGIVFLTDLLGGTPFRVASTLAMQKPGCEVITGTNLQLLLEMVL
                     EREGLSGEEFRVQALECGHRGLTSLVDELGRCHEECPVEEGI"
     gene            7707..8840
                     /gene="nagA"
                     /locus_tag="J1Y83_002874"
     CDS             7707..8840
                     /gene="nagA"
                     /locus_tag="J1Y83_002874"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HBA7631007.1"
                     /translation="MTHVLRARRLLTEEGWLDDHQLRIADGVIAAIEPIPVGVTERDV
                     ELLCPAYIDTHVHGGAGVDVMDDAPDALDKLAMHKAREGVGSWLPTTVTAPLNTIHAA
                     LKRIALRCQRGGPGAQVLGSYLEGPYFTPQNKGAHPPELFRELEIAELDQLIAVSQHT
                     LRVVALAPEKEGALQAIRHLKQQNVRVMLGHSATTWQQTRAAFDAGADGLVHCYNGMT
                     GLHHREPGMVGAGLTDKRAWLELIADGHHVHPAAMSLCCCCAKERIVLITDAMQAAGM
                     PDGHYTLCGEEVQMRGGVVRTASGGLAGSTRSVDAAVRNMVELTGVTPVEAIHMASLH
                     PARMLGVDGALGSLKPGKRASVVALDSGLHVQQIWIQGQLASF"
     gene            9190..10344
                     /locus_tag="J1Y83_002875"
     CDS             9190..10344
                     /locus_tag="J1Y83_002875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AgaS family sugar isomerase"
                     /protein_id="HBA7631008.1"
                     /translation="MPENYTPAAAATGTWTEEEIRHQPRAWIRSLTNIDALRSALNSF
                     LEPLLRKENLQIILTGAGTSAFIGDIIAPWLASHTGKNFSAVPTTDLVTNPMDYLNPA
                     HPLLLISFGRSGNSPESVAAVELANQFVPECYHLPITCNEAGALYQNAINSDNAFALL
                     MPAETHDRGFAMTSSITTMMASCLAVFAPEMINSQTFRDVADRCQAILTSLGDFSEGV
                     FGYAPWKRIVYLGSGGLQGAARESALKVLELTAGKLAAFYDSPTGFRHGPKSLVDNET
                     LVVVFVSSHPYTRQYDLDLLAELRRDNQAMRVIAIAAESNDVIAAGPHIILPPSRHFI
                     DVEQAFCFLMYAQTFALMQSLHMGNTPDTPSASGTVNRVVQGVIIHPWQA"
     gene            10357..11217
                     /gene="kbaY"
                     /locus_tag="J1Y83_002876"
     CDS             10357..11217
                     /gene="kbaY"
                     /locus_tag="J1Y83_002876"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit KbaY"
                     /protein_id="HBA7631009.1"
                     /translation="MSIISTKYLLQDAQANGYAVPAFNIHNAETIQAILEVCSEMRSP
                     VILAGTPGTFKHIALEEIYALCSAYSTTYNMPLALHLDHHESLDDIRRKVHAGVRSAM
                     IDGSHFPFAENVKLVKSVVDFCHSQDCSVEAELGRLGGVEDDMSVDAESAFLTDPQEA
                     KRFVELTGVDSLAVAIGTAHGLYSKTPKIDFQRLAEIREVVDVPLVLHGASDVPDEFV
                     RRTIELGVTKVNVATELKIAFAGAVKAWFAENPQGNDPRYYMRVGMDAMKEVVRNKIN
                     VCGSANRISA"
     gene            11384..11860
                     /gene="agaB"
                     /locus_tag="J1Y83_002877"
     CDS             11384..11860
                     /gene="agaB"
                     /locus_tag="J1Y83_002877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IIB"
                     /protein_id="HBA7631010.1"
                     /translation="MSSPNILLTRIDNRLVHGQVGVTWTSTIGANLLVVVDDVVANDD
                     IQQKLMGITAETYGFGIRFFTIEKTINVIGKAAPHQKIFLICRTPQTVRKLVEGGIDL
                     KDVNVGNMHFSEGKKQISSKVYVDDQDLTDLRFIKQRGVNVFIQDVPGDQKEQIPD"
     gene            11899..12702
                     /gene="agaC"
                     /locus_tag="J1Y83_002878"
     CDS             11899..12702
                     /gene="agaC"
                     /locus_tag="J1Y83_002878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IIC"
                     /protein_id="HBA7631011.1"
                     /translation="MHEITLLQGLSLAALVFFLGIDFWLEALFLFRPIIVCTLTGAIL
                     GDIQTGLITGGLTELAFAGLTPAGGVQPPNPIMAGLMTTVIAWSTGVDAKTAIGLGLP
                     FSLLMQYVILFFYSAFSLFMTKADKCAKEADTAAFSRLNWTTMLIVASAYAVIAFLCT
                     YLAQGAMQALVKAMPAWLTHGFEVAGGILPAVGFGLLLRVMFKAQYIPYLIAGFLFVC
                     YIQVSNLLPVAVLGAGFAVYEFFNAKSRQQAQPQPVASKNEEEDYSNGI"
     gene            12692..13483
                     /gene="agaD"
                     /locus_tag="J1Y83_002879"
     CDS             12692..13483
                     /gene="agaD"
                     /locus_tag="J1Y83_002879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708938.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IID"
                     /protein_id="HBA7631012.1"
                     /translation="MGSEISKKDITRLGFRSSLLQASFNYERMQAGGFTWAMLPILKK
                     IYKDDKPGLSAAMKDNLEFINTHPNLVGFLMGLLISMEEKGENRDTIKGLKVALFGPI
                     AGIGDAIFWFTLLPIMAGICSSFASQGNLLGPILFFAVYLLIFFLRVGWTHVGYSVGV
                     KAIDKVRENSQMIARSATILGITVIGGLIASYVHINVVTSFAIDSTHSVALQQDFFDK
                     VFPNILPMAYTLLMYYFLRVKKAHPVLLIGVTFVLSIVCSAFGIL"
     gene            13484..14251
                     /locus_tag="J1Y83_002880"
     CDS             13484..14251
                     /locus_tag="J1Y83_002880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019843039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactosamine-6-phosphate isomerase"
                     /protein_id="HBA7631013.1"
                     /translation="MERGTASGGASLLKEFHPVQTLQQVENYTALSERASEYLLAVIR
                     RKPDAVICLATGATPLLTYHYLVEQIHQQQVNVSQLTFVKLDEWVDLPLTMPGTCETF
                     LQQHIVQPLGLREDQLISFHSEEINETECERVTNLIARKGGLDLCVLGLGKNGHLGLN
                     EPGEILQPACHISQLDARTQQHEMLKTADRPVTRGITLGLKDILNAREVLLLVTGEGK
                     QDATERFLTAKVSSAIPASFLWLHNHFTCVIDEMCRR"
     gene            complement(14301..15164)
                     /gene="rsmI"
                     /locus_tag="J1Y83_002881"
     CDS             complement(14301..15164)
                     /gene="rsmI"
                     /locus_tag="J1Y83_002881"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000809248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HBA7631014.1"
                     /translation="MKQHQSADNSQGQLYIVPTPIGNLADITQRALEVLQAVDLIAAE
                     DTRHTGLLLQHFGINARLFALHDHNEQQKAETLLAKLQEGQNIALVSDAGTPLINDPG
                     YHLVRTCREAGIRVVPLPGPCAAITALSAAGLPSDRFCYEGFLPAKSKGRRDALKAIE
                     AEPRTLIFYESTHRLLDSLEDIVAVLGESRYVVLARELTKTWETIHGAPVGELLAWVK
                     EDENRRKGEMVLIVEGHKAQENDLPADALRTLALLQAELPLKKAAALAAEIHGVKKNA
                     LYKYALEQQGE"
     gene            15229..17265
                     /locus_tag="J1Y83_002882"
     CDS             15229..17265
                     /locus_tag="J1Y83_002882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708948.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator"
                     /protein_id="HBA7631015.1"
                     /translation="MVPSTFSRLKAARCLPVVLAALIFAGCGTHTPDQSTAYMQGTAQ
                     ADSAFYLQQMQQSSDDTRINWQLLAIRALVKEGKTGQAVELFNQLPQELNDSQRREKT
                     LLAAEIKLAQKDFAGAQNLLAKITPADLEQNQQARYWQAKIDASQGRPSIDLLRALIA
                     QEPLLGAKEKQQNIDATWQALSSMTQEQANTLVINADENILQGWLDLQRVWFDNRNDP
                     DMMKAGIADWQKRYPNNPGAKMLPTQLVNVKAFKPASTNKIALLLPLNGQAAVFGRTI
                     QQGFEAAKNIGTQPVAAQVAAAPAADVAEQSQPQTVDDVASPAQASVSDLTGEQPAAQ
                     PVPVSAPATSTAAVSAPANPSAELKIYDTSSQPLSQILSQVQQDGASIVVGPLLKNNV
                     EELLKSNTPLNVLALNQPENIENRVNICYFALSPEDEARDAARHIRDQGKQAPLVLIP
                     RSSLGDRVANAFAQEWQKLGGGTVLQQKFGSTSELRAGVNGGSGIALTGSPITPRETT
                     DSGMTTNNPTLQTTPTDDQFTNNGGRVDAVYIVATPGEIAFIKPMIAMRNGSQSGATL
                     YASSRSAQGTAGPDFRLEMEGLQYSEIPMLAGGNLPLMQQALSAVNNDYSLARMYAMG
                     VDAWSLANHFSQMRQVQGFEINGNTGSLTANPDCVINRKLSWLQYQQGQVVPAS"
     gene            17223..17618
                     /locus_tag="J1Y83_002883"
     CDS             17223..17618
                     /locus_tag="J1Y83_002883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HBA7631016.1"
                     /translation="MATVPTRSGSPRQLTTKQTGDAWEAQARRWLEGKGLRFIAANVN
                     ERGGEIDLIMREGRTTIFIEVRYRRSALYGGAAASVTRSKQHKLLQTARLWLARHNGS
                     FDTVDCRFDVVAFTGNEVEWIKDAFNDHS"
     gene            17638..18228
                     /gene="diaA"
                     /locus_tag="J1Y83_002884"
     CDS             17638..18228
                     /gene="diaA"
                     /locus_tag="J1Y83_002884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001483830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA initiator-associating protein DiaA"
                     /protein_id="HBA7631017.1"
                     /translation="MQERIKACFTESIQTQIAAAEALPDAISRAAMTLVQSLLNGNKI
                     LCCGNGTSAANAQHFAASMINRFETERPSLPAIALNTDNVVLTAIANDRLHDEVYAKQ
                     VRALGHAGDVLLAISTRGNSRDIVKAVEAAVTRDMTIVALTGYDGGELAGLLGPQDVE
                     IRIPSHRSARIQEMHMLTVNCLCDLIDNTLFPHQDV"
     gene            18238..18813
                     /gene="yraP"
                     /locus_tag="J1Y83_002885"
     CDS             18238..18813
                     /gene="yraP"
                     /locus_tag="J1Y83_002885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divisome-associated lipoprotein YraP"
                     /protein_id="HBA7631018.1"
                     /translation="MKALSPIAVLISALLLQGCVAAAVVGTAAVGTKAATDPRSVGTQ
                     VDDGTLEVRVNSALSKDEQIKKEARINVTAYQGKVLLVGQSPNAELSARAKQIAMGVD
                     GANEVYNEIRQGQPIGLGEASNDTWITTKVRSQLLTSDLVKSSNVKVTTENGEVFLMG
                     LVTEREAKAAADIASRVSGVKRVTTAFTFIK"
BASE COUNT         4569 a         4781 c         4962 g         4512 t
ORIGIN      
        1 taaggttgcg cgctaaagca cagatttgcg ctttaccttg ccgggaggta ctgcaatccc
       61 tgaaatgatt gacattgatc acatttctgc atttaaactc ctgacattct tatttcaccc
      121 aatgaagtta tttattttta aatgagacca ggtcctcatt ttaatagtcc ctggctggag
      181 aatattgcac aatggccaac atcaaaatca gacaagaaac gccaactgcg ttttatataa
      241 aagttcacga cacagataat gtggcaatta ttgttaatga taatggcctg aaagcaggaa
      301 cgcgttttcc ggatgggctg gaattaattg aacatattcc ccaggggcat aaagtcgcat
      361 tgctggacat tccggctaat ggtgaaatta ttcgttatgg cgaggtgatt ggttacgccg
      421 tgcgcgcaat cccacgcgga agctggatcg acgaatcaat ggttgtacta ccggaagcgc
      481 cgccgttaca tacgctgcca ctggcaacca aagtcccgga acccttaccg ccgctggaag
      541 gatacacctt tgagggctat cgcaatgccg atggcagcgt gggcaccaaa aacctgctcg
      601 gcatcaccac cagcgtccac tgtgtggcag gcgtggtgga ctacgtagta aaaatcattg
      661 aacgcgatct gctaccgaaa tacccgaacg tcgatggcgt ggtggggctg aatcatttgt
      721 acggttgtgg cgtggcgatt aacgcacccg cggcagttgt acctattcgt accattcaca
      781 atatttcgct gaaccctaac tttggcggcg aagtaatggt gattggtctg ggttgtgaaa
      841 agttgcagcc tgaacgccta ctgactggaa cggatgatgt gcaagctatt ccagtagaaa
      901 gcgccagcat tgtcagtttg caggatgaaa agcatgtcgg ttttcagtcc atggtcgagg
      961 atattttgca ggtcgccgaa tgccatctac aaaaactgaa tcaacgtcag cgagaaacct
     1021 gtccggcttc agaactggtt gtcggcatgc agtgcggtgg cagcgatgcg ttttctggcg
     1081 taacggcaaa cccggcggtt gggtatgcgt ctgatctatt ggtgcgctgc ggcgcaacgg
     1141 tgatgttttc agaagtcaca gaagtgcgtg acgcgataca tctgctgaca ccacgcgcag
     1201 tgaacgaaga ggtcggcaaa cggctgctgg aggagatgga gtggtacgat aactatctca
     1261 atatgggaaa aaccgatcgc agcgccaacc cttcgccggg gaacaagaaa ggcggtctgg
     1321 caaatgtggt ggagaaggca ctcggctcca ttgctaaatc gggtaaaagt gcaattgttg
     1381 aagtgctgtc gcccggccaa cgcccgacta aacgcggatt aatttacgcc gcgacgccag
     1441 ccagcgattt tgtctgtggc acgcaacagg tggcttcggg tatcaccgtg caagtgttta
     1501 cgaccggtcg tggtacgccg tacggcctga tggcggtacc cgtcattaaa atggcgaccc
     1561 gcaccgagct ggcgaaccgc tggtttgatt taatggatat taacgcgggc actatcgcta
     1621 ccggcgaaga aaccattgaa gaagtgggct ggaagttgtt ccactttatt ctcgacgtcg
     1681 ccagcgggaa gaagaaaact ttctcggatc aatggggact gcataaccag ctggcggtgt
     1741 ttaacccggc accggtgacc tgatttcttt tggaacgtcc ctcgcaaaac atggccttag
     1801 tgccatgttt ttattgttta aagtccacac atccattaat aatgcatttg cattacctta
     1861 gttcaagctt ataatttgag cagaaaacag gctgtaaaag gacagtaaat aatgcccgct
     1921 aatgctcgct ctaacgctgt actgaccact gaatcaaagg tcactatacg cggacaaaca
     1981 actatccccg cgccagtgcg tgaggcctta aaactgaagc ctggcctgga cagcattcat
     2041 tacgaaattc tgcctggtgg gcaagtattt atgtgccgac tgggagatga acaggaggat
     2101 catactatga atgcattttt gcgttttctg gatgcagata tccagaacaa cccgcaaaaa
     2161 actcgtccat tcgacattca acaaggaaag aaacttatcg ctggcatgga cgtcaacatt
     2221 gacgatgaga ttggcgacga cgaataatgg attttccaca aagggttaat ggttgggcgc
     2281 tatatgctca tccctgtttt caggaaacct acgatgcttt agttgccgaa gtcgaggcat
     2341 taaagggaaa agatcctgaa aattatcaga gaaaagctgc cacaaagtta ttggcggtgg
     2401 tccataaagt gattgaggag catatcacgg tcaatccatc atcaccggca ttccgtcatg
     2461 gcaagtcgtt aggctctggg aaaaataaag actggtcacg ggtaaaattt ggtgctggtc
     2521 gctatcgtct cttctttcgt tacagtgaaa aagagaaagt catcattctg ggatggatga
     2581 acgatgaaaa cacgctgcgc acctacggta aaaaaacaga tgcctatacc gtattcagta
     2641 aaatgttaaa aagaggacat cctcctgccg actgggaaag cctcacccaa gagacagaag
     2701 aaaaccattg atggtgttca caatgcccgc cgaacgcatt ggtgttgacc agcgggcatc
     2761 ctttctcatt ccccgaccag aatcacttca accccagcct ttctcagtcc ttccagacta
     2821 tccgcaggaa tgccttcatc aacaatgatc atgtcgatac gttgagtatc aatgatctta
     2881 tgtaaactag aacgattgaa cttactggaa tcggtgacca cgatgatccg ttccgcaact
     2941 tcgcacatcc gacggtttaa acgggcttca tcttcattat gcgtgctgac gccgcgctcc
     3001 agatcgatcg catctacacc aagaaacagc atatcgaagt ggtaattttg cagcgattgc
     3061 tcagcctgat caccgtaaaa agattgcgac tgacggcgca aatgcccccc ggtcatcagc
     3121 agctcaacgc cttccgcttc cagcaacgca ttagccacgt tcataccgtt ggtcatcgca
     3181 attacgtcgg tgtgcttgcg catcagacga gcaatctcaa aagtggtggt cccggagtcg
     3241 aggatcaccc gatgaccagg ctgaatcaac tcaacggcag ctttcgcaac gctgcgtttc
     3301 atcgcggtgt tcagcgcgct tttatcttcc actgatggct cgactgacgg cgttgtgcta
     3361 tcgcagatca acgcgccacc ataggcacgc acagcgatcc cctgcttttc cagaaacgcc
     3421 agatcgttgc ggatcgtcac agtagataca ccatacaacg ccgatagatc attaacttgc
     3481 acacttcctt gctggcgcag gcgttgaatg atctgttctc gtcgctcgct ggtgcctgtc
     3541 actcgcttct cacctgaagc gtcggtatta ctcatagtaa gtcctttcgt aaaactttcg
     3601 tttcatttcg ttttgcctat taacgccttt ctattaagca aatgcaagcc caccttaccc
     3661 attggcgcaa gctactctcg tttcagtggc tttcattatg tttcttttgt gaatcagatc
     3721 agaaaactat tatctttcgt tttattttta tctcaccatg acgcagtatc aactgaaaca
     3781 aaacgaaaga ttaatgtcgc agtaatctga actggagagg aaagtgaaac atctgacaga
     3841 aatggtgaga cagcacaaag cgggcaaaac aaatgggatt tacgccgttt gttccgcaca
     3901 tccgctggtg ctggaagctg caatccgcta cgccagtgca aaccaaacgc cgttactgat
     3961 tgaagcaacc tccaatcagg tggaccagtt cggcggttat accggaatga cgcccgccga
     4021 ttttcgcggc tttgtttgtc agctcgccga ctcgttgaat ttcccgcagg atgcgctgat
     4081 tctgggtggt gaccatctgg ggccaaaccg ctggcaaaac ctgcctgccg ctcaggcaat
     4141 ggccaatgcc gatgatttga ttaaaagcta cgtggcggca ggattcaaaa aaattcacct
     4201 tgattgcagc atgtcctgtc aggacgatcc gattccctta accgatggca tcgtggccga
     4261 acgcgccgcc cgtctggcga aagtggcgga agaaacctgt cttgaacact ttggcgaagc
     4321 cgatctggag tatgtcattg gtaccgaagt gccggtgcct ggcggcgcgc atgaaacctt
     4381 aagcgagctg gcggtcacca cgccggatgc cgcccgcgcc acgctggaag cccatcgtca
     4441 cgcttttgaa aagcagggtc tgaatgccat ctggccacgc attattgccc tggtggttca
     4501 acccggcgtc gaattcgatc acaccaacgt tattgattat cagtccgcca aagcgaccgc
     4561 cttaagccag atggtcgaaa actacgaaac gctgattttc gaagcgcact ctaccgatta
     4621 tcaaacaccg caatcgctgc gccagctggt gattgaccac tttgccattc tgaaagttgg
     4681 cccggcgctg acctttgctc tgcgtgaagc tctgttctct ctggcggcga ttgaagaaga
     4741 actggtgcca gcaaaagcct gttctggtct gcgtcaggtg ctggaaaacg tgatgctcga
     4801 ccgcccggaa tactggcaaa gccactacca cggtgacggc aacgcgcgtc gtctggcgcg
     4861 tggttatagc tactcggatc gcgtgcgcta ttactggccg gacagccaga ttgatgacgc
     4921 tttcgctcat ctggttcgta atctggcgga ttcaccaatt ccgctgccgc tgatcagtca
     4981 gtatctgccg ctgcagtacg tgaaagttcg ctccggcgag ctgcagccaa cgccacggga
     5041 actcattatc aaccatattc aggacatcct ggcgcagtac cacacagcct gtgaaggcca
     5101 ataagcaaaa caaagaggaa cacgctatgc caaatattgt tttaagtcgg attgatgaac
     5161 gcttgattca cggtcaggtc ggcgttcagt gggtcggatt tgcgggggca aatctggtgc
     5221 tggtagccaa cgatgaggtt gccgaagatc tggtacaaca aaacctgatg gaaatggtac
     5281 tggcagaagg gatcgccgta cgtttctgga cgctgcaaaa agtcatcgac aacattcatc
     5341 gcgccgccga tcgacagaaa atcctgctgg tttgtaaaac accggccgat ttcctgacgc
     5401 tggtgaaagg tggcgttccg gtgaatcgca ttaacgttgg caatatgcac tacgccaatg
     5461 gcaagcaaca aatcgccaaa acggtttctg tggatgcggg tgatatcgca gcatttaacg
     5521 acctgaaagc cgctggggtg gaatgcttcg ttcagggcgt cccaacagag cctgctgtgg
     5581 acctctttaa attactttga gggattcatc atggaaatca gtctgttgca ggcatttgcg
     5641 ttgggcatta tcgcctttat cgctggcctg gatatgttta acggcttaac ccatatgcac
     5701 cgcccggtgg ttctcggccc gttggtcggg ctggtacttg gcgatctgca taccggtatt
     5761 ttaaccggcg gtacgctgga actggtgtgg atggggctgg cccctctggc gggcgcacag
     5821 ccgcctaacg tgattatcgg tactatcgtc ggcacggcgt ttgccattac tactggcgtg
     5881 aaacccgatg tcgcggtcgg tgtcgccgta cctttcgctg tcgcagtaca gatggggatt
     5941 accttcctgt tctcggtgat gtccggcgtg atgtcccgat gcgaccggat ggcggagaac
     6001 gccgatacac gcggcattga acgcgtgaac tatctggcat tgctggcact cggcacgttc
     6061 tattttctct gcgctttcct gcctatctac ttcggcgcgg aacatgcaaa aaccatcatt
     6121 gatgtcctgc cgcaacgatt aattgatggc ctcggcgtcg cgggcggcat catgccagca
     6181 atcggttttg ccgtgctgct gaaaatcatg atgaaaaacg tctacatccc ctacttcatc
     6241 ctgggctttg ttgccgcggc ctggctcaag ttaccggtgc tggctattgc tgccgctgcg
     6301 ctggcgatgg cgctgatcga cctgctgcgt aaatctcctg aaccgactca acctgcggca
     6361 cagaaagagg aatttgaaga tggcatctaa tcaaaccacc ctgccgaacg tcagcgaaaa
     6421 tgaagaaaca ctgctgactg gcgtcaatga aaacgtgtat gaagatcaga gcattggcgc
     6481 ggagctgacg aaaaaagata tcaaccgcgt cgcctggcgt tccatgctgt tacaggcttc
     6541 tttcaactac gaacgtatgc aggcttccgg ctggctgtac ggcctgctgc cagcactgaa
     6601 aaagatccac actaataaac gcgacctggc gcgcgccatg aaggggcata tgggtttctt
     6661 caatacccat ccgtttctgg tgacgtttgt tatcggcatt atccttgcga tggagcgttc
     6721 taagcaggac gttaacagta ttcagagcac caaaattgcc gtcggtgcgc cgctcggcgg
     6781 gattggcgat gcgatgttct ggctaacgct actgccgatt tgtggcggga taggtgcaag
     6841 cctcgcgcta caaggctcta ttcttggcgc tgtcgtcttt attgtgctgt tcaacgtggt
     6901 acacctgggg ctgcgttttg gtctggcgca ttatgcttat cgcatgggcg tggcggcgat
     6961 tccactcatt aaagcgaata ccaaaaaagt cggccatgcg gcatctatcg ttgggatgac
     7021 ggtaatcggc gcgctggtgg caacctatgt tcgtttaagc accacgctgg aaatcacagc
     7081 gggcgacgca gtggttaagt tacaggctga tgttatcgac aaactgatgc cagccttctt
     7141 accgctggtc tacaccctga ccatgttctg gctggtacgc cgcggctgga gtccgctgcg
     7201 cctgattgca gtgacagtgg ttctcggcat cgtcggtaaa ttctgccatt tcctttaaat
     7261 acaatgaggt attagatgtt aagtattatt ttgacagggc atggcgggtt tgccagcggt
     7321 atggaaaaag caatgaagca aattctcggt gagcaatcgc agtttatcgc catcgatttt
     7381 ccggaaacct caagcaccgc gcagcttaca tcgcagcttg aagaggccat cgcccaactg
     7441 gattgtgaag acggcattgt ttttctgacg gatttgctgg gcggcacgcc gtttcgcgta
     7501 gcttcaacgc tggcgatgca aaaaccgggc tgtgaagtga tcaccggcac aaatttgcaa
     7561 ctattactgg aaatggtgct ggaacgcgaa gggttaagcg gtgaagagtt ccgtgtgcag
     7621 gcgctggagt gtggacaccg tgggctaacc agtctggtgg acgaacttgg tcgctgccat
     7681 gaggaatgtc cggtcgagga aggaatatga cacacgttct gcgcgccaga aggctgctga
     7741 ctgaagaggg atggctcgat gaccatcagt tgcgtattgc tgacggtgta atcgcagcaa
     7801 tcgaaccgat tccagtgggc gtgactgaac gcgatgtgga actgctctgc cccgcttaca
     7861 tcgacaccca tgtacacggt ggtgcgggcg ttgatgtaat ggatgacgcg ccggatgcgc
     7921 tcgacaagct ggcaatgcac aaggcacgcg aaggtgtcgg cagttggtta ccgaccaccg
     7981 taaccgcgcc gcttaatacc attcatgcgg cgctgaaacg tattgctcta cgctgccagc
     8041 gtggcggacc tggtgcacaa gtgctgggga gttatctcga aggaccgtac ttcacgccgc
     8101 agaataaagg cgcgcatccg ccggagctgt ttcgcgagct tgaaattgcc gagctggatc
     8161 aattgattgc cgtttctcag cataccttgc gcgtggtagc gctggcacca gaaaaagaag
     8221 gggcattgca ggccatccgc catcttaaac agcaaaacgt acgggtgatg ctggggcata
     8281 gcgcgacgac ctggcaacaa acccgtgccg cgtttgatgc tggtgccgac ggcctggtgc
     8341 attgctataa cgggatgaca ggtttacatc accgcgaacc gggaatggtt ggcgcgggat
     8401 taacggacaa gcgcgcctgg ctggaactga tagccgatgg tcatcatgta catccggcgg
     8461 caatgtcgct ctgttgttgc tgtgcgaaag agaggatcgt actgatcacc gacgcgatgc
     8521 aggcagccgg gatgccggat ggtcactata cgttatgtgg tgaagaagtg cagatgcgcg
     8581 gtggcgttgt ccgtaccgcg tccggtgggc tggcgggcag tacgcggtct gtcgatgcgg
     8641 cagtgcgcaa catggtcgag ttgacgggcg taacgcctgt ggaagccatc catatggcgt
     8701 cgctgcatcc ggcgcgaatg ctgggtgttg atggtgctct gggatcgctt aaaccgggca
     8761 aacgcgccag cgtcgttgcg ctggatagcg ggctgcatgt gcaacaaatc tggattcagg
     8821 gtcaattagc ttcgttttga tggtttgctc ctttattagg ccttcacttc acccgtaagg
     8881 cctttctttt tctttcgttt tgatctgtgc agcggtgtcg gatgcgacgc taatgcgcct
     8941 tatccgacct acggttggtg accgtaaggc cggataaagt atttacgccg catccagcaa
     9001 tcccttttac ttcccttacc ttttctttca acgatcacat atttcgtttt atttcctttt
     9061 tctccattga actttcagtt tcttttctat agattttaac caacgaaaga aatcaccaag
     9121 tgaaatgaaa cgaaaggcaa gcgaaagcga caacgccgac gtcaagttca tcagactaag
     9181 gattgagtta tgccagaaaa ttacacccct gctgctgccg caaccggtac atggactgaa
     9241 gaagagatcc gccatcagcc tcgcgcatgg atccgttcac tcaccaacat tgacgcgcta
     9301 cgttccgcgc tcaatagctt ccttgaaccg ttactgcgca aagagaatct gcaaatcatc
     9361 ctgaccggag ccggaacatc ggcatttatc ggtgacatca tcgcgccgtg gctcgccagc
     9421 cataccggta aaaacttcag cgccgtaccg accaccgatc tggtcactaa tccgatggac
     9481 tacctgaatc cagctcatcc actactgttg atctccttcg gtcgatccgg caacagcccg
     9541 gaaagcgtcg ccgccgtgga actggcaaat caatttgtac cagaatgcta tcacctgccg
     9601 atcacctgca acgaagcggg cgctctttac caaaacgcga tcaacagcga caacgcgttt
     9661 gccctgctga tgcccgcaga aacacacgat cgcggcttcg cgatgaccag cagcattacc
     9721 accatgatgg ccagctgcct cgcggttttc gcacctgaga tgatcaacag ccaaaccttc
     9781 cgcgacgtgg cggatcgttg ccaggcgatc ctgacctcac tgggcgattt cagcgaaggt
     9841 gtgtttggtt acgcaccgtg gaaacggatc gtttatctcg gcagcggtgg cttacagggc
     9901 gcagcacgcg agtcggcgct gaaagtgctg gaactgacag cgggtaaact ggcggccttc
     9961 tatgattccc cgaccggatt ccgtcatggc ccgaaatcgc tggtcgataa cgaaacgctg
    10021 gtggtggtgt ttgtctccag ccacccttac acccgtcagt atgatcttga tctgctggca
    10081 gaactccgcc gcgataacca ggcaatgcgc gtaatcgcca tcgccgcgga aagcaacgac
    10141 gttattgccg ccggtccaca tatcatcctg ccgccgtccc gtcactttat cgacgttgag
    10201 caggcatttt gcttcctgat gtacgcccag acatttgcac tgatgcagtc gctgcacatg
    10261 ggtaatacgc cggatacccc atcagccagt ggcaccgtta accgcgtggt gcaaggcgta
    10321 atcattcatc cgtggcaggc ataagaggat cgcattatga gcattatctc caccaaatat
    10381 ctgttacagg acgcccaggc caatggctac gcggtgcctg cctttaacat tcataacgcc
    10441 gagacgatcc aggcgatcct cgaagtgtgc agtgaaatgc gatcgccggt gatcctcgcc
    10501 gggacgccgg ggacctttaa gcatatcgcg ctggaagaga tctacgccct gtgtagcgcc
    10561 tattccacga cctataacat gccgctggca ctgcatcttg atcaccacga atcgctggat
    10621 gatattcgcc gtaaagtcca cgcaggcgtg cgcagcgcga tgatcgacgg cagtcacttc
    10681 ccgtttgccg agaacgtgaa gctggtgaaa tcggttgtcg acttctgcca ctcccaggat
    10741 tgcagcgtgg aagcagaact gggccgcctc ggcggtgttg aagatgacat gagcgttgac
    10801 gccgaaagtg cattcctgac tgatccacaa gaagctaaac gctttgtcga actgactggc
    10861 gtcgacagcc tggcggtagc gattggtacg gcgcacggct tatacagcaa aacgccgaag
    10921 attgatttcc agcggctggc ggaaattcgt gaagtggtgg atgttcctct ggtgctgcat
    10981 ggtgccagcg atgtcccgga tgaatttgtc cgtcgcacta ttgaacttgg cgtaacaaaa
    11041 gtgaacgttg ccacagaatt aaaaatagcc ttcgctggcg cggttaaagc ctggtttgcg
    11101 gaaaatccgc agggtaatga tcctcgttat tatatgcgcg tcggaatgga tgcgatgaaa
    11161 gaagttgtca gaaataaaat taatgtctgt ggttcagcga atcgaatttc agcataatca
    11221 ttagtttttt atgatttatc ccaatgtact tcccgattaa tatcggggag tgccttaatg
    11281 gtaaaggaga taactaaacc ttaataaata catcagtaca atatcgcaac aataatatat
    11341 ttaaaaagat tatattattc aactttatgg tgaggattac ataatgagca gtccaaatat
    11401 tctcttaacc cgtattgata accgtctggt tcacggtcag gttggcgtga cctggacatc
    11461 caccatcggt gcgaatctgt tagtagtcgt ggatgatgtt gtcgctaacg atgatattca
    11521 acagaaatta atgggtatta ccgcagaaac ctacggcttt ggcattcgtt tctttactat
    11581 cgaaaaaacc attaacgtca tcggcaaagc tgcaccacat cagaagatct tcctgatttg
    11641 ccgtacgcca caaacggtac gtaaattggt agaaggtggt attgacctga aagatgtcaa
    11701 cgtcggcaat atgcatttct cggaagggaa aaagcaaatc agcagtaaag tttatgtcga
    11761 tgaccaggat ctcacggatt tacgttttat taaacaacgt ggcgtaaatg ttttcattca
    11821 ggacgtccct ggcgatcaaa aagaacaaat ccctgactaa atctaaaata gccttaatat
    11881 tggtttgagg taataaaaat gcatgaaata accctactcc agggattatc cctggcggcg
    11941 ttagttttct ttctggggat tgatttttgg ctggaagcct tatttttatt ccgcccgata
    12001 atcgtttgta cccttacagg tgctattctc ggtgatattc agactggctt aattaccggt
    12061 ggcctgacag agttggcttt cgccggatta acccctgcag gtggtgttca gccgcccaac
    12121 cctattatgg cgggtctgat gaccactgtc attgcatggt ctacgggcgt tgatgccaaa
    12181 acggcaattg gtcttggcct gccgtttagt ttgttaatgc agtacgtcat tctgttcttc
    12241 tattccgctt tctcattatt tatgaccaaa gccgataaat gcgcaaaaga ggcggatacg
    12301 gcagcgtttt cccgacttaa ctggacaacg atgctcatcg tcgcttcagc gtatgcggtg
    12361 attgctttcc tctgtactta cctggcacaa ggggcgatgc aggcgctggt gaaagcgatg
    12421 cccgcctggc tgacccacgg ctttgaagtg gctggcggta ttctgcctgc tgttggtttt
    12481 ggcttgctgc tgcgcgtgat gttcaaagca caatatatcc cttacctgat cgccggtttc
    12541 ctgtttgttt gctacatcca ggtcagcaac ctgttgccgg ttgccgtact gggcgcaggc
    12601 tttgcggtgt atgagttttt caatgcgaaa tcccggcagc aagcgcaacc gcagcccgtt
    12661 gccagtaaaa atgaagaaga ggactacagc aatgggatct gaaatcagta aaaaagatat
    12721 cacccgtctg ggctttcgtt cgtcactgct acaagcaagc tttaactacg aaaggatgca
    12781 ggcgggcggt tttacctggg cgatgttgcc gatcctgaaa aagatttata aggacgacaa
    12841 accgggccta agcgcggcga tgaaagataa cctcgaattt attaataccc accctaatct
    12901 ggtcggattc ctgatggggt tattaatttc gatggaagaa aaaggagaaa accgcgacac
    12961 cattaaaggc ctcaaagtgg cgctgtttgg cccaatcgcc gggattggcg atgcgatttt
    13021 ctggtttacc ttgttgccaa ttatggcggg gatttgctca tcgtttgcca gtcaggggaa
    13081 cctgttggga ccgattttgt ttttcgccgt ttacctgctt atctttttcc tgcgcgtcgg
    13141 ctggacccac gtcggttatt cagtcggcgt gaaggctatc gataaagtgc gagagaactc
    13201 gcagatgatt gctcgttcgg caaccatcct cgggatcaca gtaatcggcg ggctgatcgc
    13261 ttcgtatgtg catattaacg tggtgacgtc gtttgccatc gacagtaccc acagcgtcgc
    13321 actgcaacag gatttcttcg ataaagtctt cccgaatatt ttaccgatgg cctacaccct
    13381 gctgatgtat tacttcctgc gggtgaaaaa agcgcatccg gtgctgttaa tcggcgtgac
    13441 gtttgttctt tctattgttt gttctgcatt cggcattttg taaatggaac gaggcaccgc
    13501 gtctggcggt gcctctttac ttaaggaatt tcatcctgtg caaacccttc aacaagttga
    13561 aaactatacg gcgttaagtg aacgtgccag cgaatattta ttggccgtga tccgtcgcaa
    13621 accggatgcc gtgatttgcc tggcgaccgg agccacgcca ttactgacgt atcattatct
    13681 ggtagaacaa atccaccagc agcaggttaa tgtcagccag ctcaccttcg tgaagctcga
    13741 cgaatgggtg gatctgccat taacgatgcc tggtacctgc gaaactttcc ttcaacagca
    13801 tatcgtgcag ccgctggggc tacgtgaaga ccagctcatc agctttcact ccgaagagat
    13861 aaatgagaca gagtgcgaac gggtaacgaa cctgattgcg cgcaaaggcg gtctggattt
    13921 atgcgttctc ggattgggga aaaacggtca tcttgggctg aacgaaccgg gagaaatcct
    13981 gcaaccggcc tgccatatca gtcaacttga tgccagaaca cagcaacatg agatgttaaa
    14041 aaccgcggac cgtcccgtga ctcgtgggat caccttaggc ctgaaggata ttctcaatgc
    14101 ccgcgaagtt ttgttactgg tgactggcga aggaaaacag gatgcgacag aacgttttct
    14161 cacggctaaa gtctcctccg ctatcccggc ttcattttta tggctgcata accatttcac
    14221 atgcgtgatt gatgaaatgt gtcgtcgtta actcgccagg taaaggcgtt gtagcgctat
    14281 tcctgctgcg acgcctgtaa tcactctccc tgctgctcca gcgcatactt atacagcgca
    14341 tttttcttca cgccgtgaat ttctgcggcc agcgccgccg cttttttcag cggcagttct
    14401 gcctgtagca gcgccagcgt gcgcagggca tcagcaggca agtcattttc ctgcgcttta
    14461 tggccttcga caatcagcac catttcgcct ttgcgacggt tttcatcttc ttttacccac
    14521 gccagcagct cgccaacggg cgcgccgtga atggtttccc aggttttggt tagctcacgc
    14581 gccagaacca cgtaacggga ttcgcctaat accgcaacga tatcttccag gctatctaac
    14641 agacggtggg tagattcata aaaaatcagc gtgcgcggct ccgcttcaat agcttttagc
    14701 gcatcacggc ggccttttga tttggcaggt aaaaagcctt cataacaaaa acgatcggat
    14761 ggtaaacccg cggcgcttaa cgcagtgata gcagcacacg gccctggtag cggcaccacg
    14821 cgaatccccg cttcacgaca ggtacgcacc agatggtagc caggatcgtt aattagcggc
    14881 gttccggcat cggaaaccag cgcaatgttt tgcccctctt gcagcttcgc cagcagcgtt
    14941 tcggcttttt gttgttcatt atggtcgtgc agcgcaaaca accgggcatt aatcccaaaa
    15001 tgttgcagca ataaaccggt gtgacgcgta tcctcggcgg caatcagatc aacggcctgt
    15061 aatacctcta atgcacgctg ggtgatatcc gccagattgc cgattggcgt cggtacaatg
    15121 taaagctgcc cctgagaatt atccgccgat tggtgttgtt tcattgtgtc gtccgtattg
    15181 ccgatttaat attgagcatt gcgtaaaaaa aatatcactg gatacattat ggtaccctca
    15241 acattttctc gtttgaaagc cgcgcgttgt ctgcctgttg ttctggcagc cctgattttc
    15301 gccggttgtg gcacccatac tcccgatcag tccactgctt atatgcaggg cacggcgcag
    15361 gctgattctg ccttttacct gcaacagatg cagcaaagtt ctgatgatac caggatcaac
    15421 tggcaattac tcgccattcg tgcactggtg aaagaaggta aaaccggaca ggcggtggag
    15481 ttgtttaacc aactaccgca agaactgaac gattctcagc gtcgcgagaa aacactgctg
    15541 gcggcagaga ttaaactggc gcagaaagat tttgctggcg cgcaaaactt gctggcgaaa
    15601 atcacacctg cagatttaga acaaaaccag caagcgcgtt actggcaggc aaaaatcgat
    15661 gccagccagg ggcgtccttc cattgattta ctgcgcgcgt taattgctca ggaaccgctg
    15721 ctcggtgcga aagaaaagca gcagaatatt gacgccacat ggcaggcgct ctcctccatg
    15781 actcaggagc aggcgaatac gctggtgatc aacgccgacg aaaatattct gcaaggctgg
    15841 ctggatttgc agcgcgtctg gtttgataac cgtaacgatc ctgacatgat gaaagctggg
    15901 atcgccgact ggcagaaacg ttatccgaac aacccgggcg cgaaaatgct gccaacgcag
    15961 ttggttaacg taaaagcgtt taaaccagca tcgaccaaca aaatcgccct gctgttgccg
    16021 ctgaatggcc aggcagcggt atttggtcgc actattcagc aaggctttga agcggcgaaa
    16081 aatatcggca ctcagccagt ggcggcccag gtagctgccg cacctgccgc agacgtagca
    16141 gaacaatctc agccgcaaac cgtggatgac gttgccagcc cggcacaagc ctcggttagc
    16201 gatctgaccg gtgaacagcc tgcggcccag ccggtgcctg taagcgcccc ggcgacaagc
    16261 accgcagcgg taagcgcacc cgcaaatcca tccgcagagc tgaaaatcta cgatacctca
    16321 tcacaaccac ttagccagat cttaagccag gttcagcagg atggcgcgag tattgtggtc
    16381 ggtccgttgc tgaaaaataa cgttgaagag ttgctgaaga gcaacactcc gctgaacgtg
    16441 ctggcactga accagccgga gaacatcgaa aaccgcgtca atatttgtta cttcgcgctt
    16501 tcaccggaag acgaagcgcg cgatgcagcg cgtcatattc gtgaccaggg taaacaagcg
    16561 ccgctggtgc tgatcccacg cagttcattg ggcgatcgcg tagccaatgc gtttgcgcaa
    16621 gagtggcaga aactgggcgg cggcaccgtt ctgcaacaaa aatttggttc caccagcgag
    16681 ttacgcgcgg gtgttaacgg cggttcgggt atcgctttaa cgggtagccc gattactccc
    16741 agagaaacaa ccgactccgg catgacgacc aacaatccaa cgctgcaaac cacaccaacc
    16801 gatgatcagt tcaccaataa tggcggtcgt gtcgatgcgg tgtacattgt ggcaacgccg
    16861 ggtgaaatcg cttttatcaa accaatgatc gccatgcgta acggtagcca gagcggtgca
    16921 acgctgtacg ccagctcccg cagtgcgcaa gggaccgctg gcccggattt ccgcctggag
    16981 atggaaggct tgcagtacag cgaaatcccg atgctggcag gcggtaatct gccgttaatg
    17041 cagcaggcgc tcagcgcggt gaacaacgat tattcgctgg ctcgcatgta tgcgatgggc
    17101 gtcgatgcct ggtcgttggc aaatcatttc tcacaaatgc gccaggttca gggttttgaa
    17161 atcaacggta ataccggaag cctgacggca aacccggatt gcgtgattaa caggaagtta
    17221 tcatggctac agtaccaaca aggtcaggta gtccccgcca gttaaccacc aaacagaccg
    17281 gcgatgcgtg ggaagcacaa gcgcgtcgct ggctggaagg caaaggacta cggtttatcg
    17341 ccgctaacgt gaacgagcgt ggcggcgaga tcgatctgat aatgcgtgaa ggccggacca
    17401 ccatttttat cgaggtgcgt taccgccgct ctgcgcttta tggcggcgcg gcagccagtg
    17461 tgacccgcag caaacaacac aaattattac agactgcccg cttgtggctc gcgcgtcata
    17521 atgggagttt tgatactgtg gattgccggt tcgatgtggt agccttcacc gggaatgagg
    17581 ttgagtggat taaggatgcc tttaatgacc actcataatt aaggtttaag gattagcgtg
    17641 caagaaagaa ttaaagcttg cttcactgaa agcattcaaa ctcaaattgc ggcggcagag
    17701 gcgcttccgg atgccatctc ccgtgcagcc atgacgctgg ttcagtctct gctcaatggc
    17761 aacaaaatcc tctgttgtgg taatggaact tccgctgcca atgcacagca ttttgctgcc
    17821 agcatgatca accgtttcga aacggagcgg cccagcttac ctgccattgc actaaatact
    17881 gataatgttg tcttaacggc aattgccaac gatcgcttac atgatgaagt gtatgcaaaa
    17941 caggtgcggg cgctgggcca tgcgggagat gtattgttag ccatttccac ccgtggcaac
    18001 agccgcgata ttgtaaaagc ggtagaagct gccgttacgc gtgatatgac cattgtggca
    18061 ttaaccggct atgacggcgg tgaacttgca ggtttgttag ggccacagga tgtggagatc
    18121 cgcattcctt cgcatcgtag tgctcgcatt caggaaatgc atatgctgac ggtaaattgc
    18181 ctgtgcgatc tgatcgataa cacgcttttc cctcaccagg atgtttaagg agaatacatg
    18241 aaggcattat cgccaatcgc agtccttatt tccgcgctgc tgttgcaagg ttgtgttgcc
    18301 gctgccgtag tgggtactgc tgctgtgggt accaaagccg caactgaccc acgcagtgtc
    18361 ggcacccagg tagacgatgg taccctggaa gtgcgcgtga acagcgcatt gtcgaaagac
    18421 gaacaaatta agaaagaagc gcgcattaat gtaacggcct atcagggcaa agtgctgctg
    18481 gttgggcagt caccaaacgc tgaactttcg gctcgcgcca aacagattgc tatgggcgta
    18541 gacggtgcca acgaagtgta taacgagatt cgtcagggcc agccgattgg tctgggcgaa
    18601 gcgtctaacg atacgtggat caccaccaaa gtacgttcgc agctgttaac cagcgacctg
    18661 gtgaaatcgt ccaacgtgaa agtgaccacc gaaaacggtg aagtgttcct gatggggctg
    18721 gtgactgaac gtgaagcgaa agcggcggca gatattgcca gccgggtaag cggcgtgaag
    18781 cgggtaacta cggcgtttac gtttattaaa tagcagccct tgta
//
LOCUS       DADSYM010000097        18821 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2415
            SAMN08039579-rid11723173.denovo.097, whole genome shotgun sequence.
ACCESSION   DADSYM010000097 DADSYM010000000
VERSION     DADSYM010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039579
            Sequence Read Archive: SRR6362533
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18821)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18821)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980162.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 03:39:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,484
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 4,347
            CDSs (with protein)               :: 4,347
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 43 of 85
            Pseudo Genes (incomplete)         :: 37 of 85
            Pseudo Genes (internal stop)      :: 19 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18821
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039579-rid11723173.denovo.097"
                     /strain="LGE2415"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8302 N 95.9628 W"
                     /collection_date="2015-08-24"
     gene            complement(328..594)
                     /locus_tag="J1Y83_002886"
                     /pseudo
     CDS             complement(328..594)
                     /locus_tag="J1Y83_002886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226947.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
     gene            complement(866..2188)
                     /gene="hipA"
                     /locus_tag="J1Y83_002887"
     CDS             complement(866..2188)
                     /gene="hipA"
                     /locus_tag="J1Y83_002887"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system serine/threonine
                     protein kinase toxin HipA"
                     /protein_id="HBA7631019.1"
                     /translation="MPKLVTWMNNQRVGELTKLANGAHTFKYAPEWLASRYARPLSLS
                     LPLQRGNITSDAVFNFFDNLLPDSPIVRDRIVKRYHAKSRQPFDLLSEIGRDSVGAVT
                     LIPEDETVTCPIMAWEKLTEARLEEVLTAYKADIPLGMIREENDFRISVAGAQEKTAL
                     LRIGNDWCIPKGITPTTHIIKLPIGEIRQPNATLDLSQSVDNEYYCLLLAKELGLNVP
                     DAEIIKAGRVRALAVERFDRRWNTERTVLLRLPQEDMCQTFGLPSSVKYESDGGPGIA
                     QIMAFLMGSSEALKDRYDFMKFQVFQWLIGATDGHAKNFSVFIQAGGSYRLTPFYDII
                     SAFPVLGGTGIHISDLKLAMGLNASKGKKTAIDKIYPRHFLATAKVLKFPEVQMHEIL
                     SDFARMIPAALDNVKTSLPTDFPENVVTAVETNVLRLHGRLSREYGIK"
     gene            complement(2188..2454)
                     /gene="hipB"
                     /locus_tag="J1Y83_002888"
     CDS             complement(2188..2454)
                     /gene="hipB"
                     /locus_tag="J1Y83_002888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin HipB"
                     /protein_id="HBA7631020.1"
                     /translation="MMSFQKIYSPMQLANAMKLVRQQNGWTQSELAKKIGIKQATISN
                     FENNPDNTSLTTFFKILQSLELSMTLCDAKNASPEATEQQDLEW"
     gene            2762..3520
                     /gene="tam"
                     /locus_tag="J1Y83_002889"
     CDS             2762..3520
                     /gene="tam"
                     /locus_tag="J1Y83_002889"
                     /EC_number="2.1.1.144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trans-aconitate 2-methyltransferase"
                     /protein_id="HBA7631021.1"
                     /translation="MSDWNPSLYLHFAAERSRPAVELLARVPLENIEYIADLGCGPGN
                     STALLHQRWPAARITGIDSSPAMIAEARSALPDCLFVEADIRNWQPEQALDLIFANAS
                     LQWLPDHYELFPHLVSLLSPLGVLAVQMPDNWLEPTHVLMREVAWEQNYPDRGREPLA
                     GVHAYYDILSEAGCEVDIWRTTYYHQMPSHQAIIDWVTATGLRPWLQDLTESEQQHFL
                     TRYHQMLEEQYPLQENGQILLAFPRLFIVARRTE"
     gene            complement(3524..4438)
                     /locus_tag="J1Y83_002890"
     CDS             complement(3524..4438)
                     /locus_tag="J1Y83_002890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416037.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bestrophin family inner membrane protein"
                     /protein_id="HBA7631022.1"
                     /translation="MIVRPQQHWLRRIFVWHGSVLSKISSRLLLNFLFSIAVIFMLPW
                     YTHLGIKFTLAPFSILGVAIAIFLGFRNNAGYARYVEARKLWGQLMIASRSLLREVKT
                     TLPDSASVREFARLQIAFAHCLRMTLRKQPQAEVLAHYLKTEDLQRVLASNSPANRIL
                     LIMGEWLAVQRRNGQLSDILFISLNDRLNDISAVLAGCERIAYTPIPFAYTLILHRTV
                     YLFCIMLPFALVVDLHYMTPFISVLISYTFISLDCLAEELEDPFGTENNDLPLDAICN
                     AIEIDLLQMNDEAEIPAKILPDRHYQLT"
     gene            complement(4635..6086)
                     /locus_tag="J1Y83_002891"
     CDS             complement(4635..6086)
                     /locus_tag="J1Y83_002891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate reductase"
                     /protein_id="HBA7631023.1"
                     /translation="MKTLNRRDFPGAQYPERIIQFGEGNFLRAFVDWQIDLLNEHTDL
                     NSGVVVVRPIETSFPPSLSTQDGLYTTIIRGLNEKGEAVSDARLIRSVNREISVYSEY
                     DEFLKLAHNPEMRFVFSNTTEAGISYHAGDKFDDAPAVSYPAKLTRLLFERFSHFNGA
                     LDKGWIIIPCELIDYNGDALRELVLRYAQEWALPEAFIQWLDQANSFCSTLVDRIVTG
                     YPRDEVAKLEEELGYHDGFLDTAEHFYLFVIQGPKSLATELRLDKYPLNVLIVDDIKP
                     YKERKVAILNGAHTALVPVAFQAGLDTVGEAMNDAEICAFVEKAIYEEIIPVLDLPRD
                     ELESFASAVTGRFRNPYIKHQLLSIALNGMTKFRTRILPQLLAGQKANGTLPARLTFA
                     LAALIAFYRGERNGETYPVQDDAHWLERYQQLWSQYRDRVIGTQELVAIVLAEKDHWE
                     QDLTQVPGLVEQVANDLDAILEKGMREAVRPLC"
     gene            complement(6313..7731)
                     /locus_tag="J1Y83_002892"
     CDS             complement(6313..7731)
                     /locus_tag="J1Y83_002892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HBA7631024.1"
                     /translation="MHVQPISTFRLFQEGHLLRNSIAIFVLTTLFYFIGAELRLVHEL
                     SLFWPLNGVMAGVFARYVWLNRLHYYAISYVAMLVYDAITTEWGLVSLAINFSNMMFI
                     VTVALLVARDKRLGKNKYEPVSALRLFNYCLLAALLCAIVGAIGSVSIDSLDFWPLLA
                     DWFSEQFSTGVLIVPCMLTLAIPGVLPRFKAEQMMPAIALIVSVIASVVIGGAGSLAF
                     PLPALIWCAVRYTPQVTCLLTFVTGAVEVVLVANSVIDISVGSPFSIPQMFSARLGIA
                     TMAICPIMVSFSVAAINSLMKQVALRADFDFLTQVYSRSGLYEALKSPSLKQTQHLTV
                     MLLDIDYFKSINDNYGHECGDKVLSVFAQHIQKIVGDKGLVARMGGEEFAVAVPSVNP
                     VDGLLMAEKIRKGVELQPFTWQQKTLYLTVSIGVGSGCASYRTLTDDFNKLMVEADTC
                     LYRSKKDGRNRTSTMRYGEEVV"
     gene            complement(7870..8229)
                     /locus_tag="J1Y83_002893"
     CDS             complement(7870..8229)
                     /locus_tag="J1Y83_002893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4186 domain-containing protein"
                     /protein_id="HBA7631025.1"
                     /translation="MQSLDPLFARLSRSKFRSRFRLGVKERQYCLEKGAPVIEQHAAD
                     FVAKRLAPALPANDGKQTPMRGHPVFIAQHATATCCRGCLAKWHNIPQGEALSEEQQR
                     YIVTVIYHWLVIQMNQP"
     gene            complement(8229..9155)
                     /gene="glsB"
                     /locus_tag="J1Y83_002894"
     CDS             complement(8229..9155)
                     /gene="glsB"
                     /locus_tag="J1Y83_002894"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase B"
                     /protein_id="HBA7631026.1"
                     /translation="MAVAMDNAILENILRQVRPLIGQGKVADYIPALATVDGSRLGIA
                     ICTVDGQLFQAGDAQERFSIQSISKVLSLVVAMRHYSEEEIWQRVGKDPSGSPFNSLV
                     QLEMEQGIPRNPFINAGALVVCDMLQGRLSAPRQRMLEVVRGLSGVSDISYDTVVARS
                     EFEHSARNAAIAWLMKSFGNFHHDVTTVLQNYFHYCALKMSCVELARTFVFLANQGKA
                     IHIDEPVVTPMQARQINALMATSGMYQNAGEFAWRVGLPAKSGVGGGIVAIVPHEMAI
                     AVWSPELDDAGNSLAGIAVLEQLTKQLGRSVY"
     gene            complement(9219..10607)
                     /gene="sad"
                     /locus_tag="J1Y83_002895"
     CDS             complement(9219..10607)
                     /gene="sad"
                     /locus_tag="J1Y83_002895"
                     /EC_number="1.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate-semialdehyde dehydrogenase"
                     /protein_id="HBA7631027.1"
                     /translation="MTITPATHAISINPATGEQLSVLPWAGVDDIENALQLAAAGFRD
                     WREANIDYRAEKLRDIGKALRARSEEMAQMITREMGKPINQARAEVAKSANLCDWYAE
                     HGPAMLKAEPTLVENQQAVIEYRALGTILAIMPWNFPLWQVMRGAVPIILAGNGYLLK
                     HAPNVMGCAQLIAQVFKDAGIPQGVYGWLNADNDGVSLMIKDSRIAAVTVTGSVRAGA
                     AIGAQAGAALKKCVLELGGSDPFIVLNDADLELAVKAAVAGRYQNTGQVCAAAKRFII
                     EEGIASAFTERFVAAAAALKMGDPRDEENALGPMARFDLRDELHHQVEKTLAQGARLL
                     LGGEKMAGAGNYYPPTVLANVTPEMTAFREEMFGPVAAITIAKDAEHALELANDSEFG
                     LSATIFTTDETQARQMAARLECGGVFINGYCASDARVAFGGVKKSGFGRELSHFGLHE
                     FCNIQTVWKDRV"
     gene            10708..11589
                     /locus_tag="J1Y83_002896"
     CDS             10708..11589
                     /locus_tag="J1Y83_002896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBA7631028.1"
                     /translation="MDLTQLEMFNAVAEAGSITQAAAKVHRVPSNLTTRLRQLETELG
                     VDLFIRENQRLRLSPAGHNFLRYSQQILTLVDEARSVVAGDEPQGLFSLGSLESTAAV
                     RIPATLAEFNRRYPKIQFSLSTGPSGTMLEGVLEGKLNAAFIDGPINHTAIDGIPVYR
                     EELMIVTPQGHAPVTRASQVNGSNIYAFRANCSYRRHFESWFHADGAAPGTIHEMESY
                     HGMLACVVAGAGIALIPRSMLESMPGHHQVEAWPLAEQWRWLTTWLVWRRGAKTRPLE
                     AFIQLLDAPDSAKQGYQ"
     gene            11667..11750
                     /gene="yneK"
                     /locus_tag="J1Y83_002897"
                     /pseudo
     CDS             11667..11750
                     /gene="yneK"
                     /locus_tag="J1Y83_002897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416044.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="putative protein YneK"
     gene            11928..13118
                     /gene="ydeA"
                     /locus_tag="J1Y83_002898"
     CDS             11928..13118
                     /gene="ydeA"
                     /locus_tag="J1Y83_002898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose MFS transporter"
                     /protein_id="HBA7631029.1"
                     /translation="MTTNTVSRKVAWLRVVTLAVAAFIFNTTEFVPVGLLSDIAHSFH
                     MQTAQVGIMLTIYAWVVALMSLPFMLMTSQVERRKLLICLFVVFIASHVLSFLSWSFT
                     VLVISRIGVAFAHAIFWSITASLAIRMAPAGKRAQALSLIATGTALAMVLGLPLGRIV
                     GQYFGWRMTFFAIGIGALITLLCLIKLLPLLPSEHSGSLKSLPLLFRRPALMSIYLLT
                     VVVVTAHYTAYSYIEPFVQNIAGFSANFATALLLLLGGAGIIGSVIFGKLGNQYASAL
                     VSTAIALLLVCLALLLPAANSEIHLGMLSIFWGIAMMIIGLGMQVKVLALAPDATDVA
                     MALFSGIFNIGIGAGALVGNQVSLHWSMSMIGYVGAVPAFAALIWSIIIFRRWPVTLE
                     EQTQ"
     gene            complement(13143..13808)
                     /gene="marC"
                     /locus_tag="J1Y83_002899"
     CDS             complement(13143..13808)
                     /gene="marC"
                     /locus_tag="J1Y83_002899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter MarC"
                     /protein_id="HBA7631030.1"
                     /translation="MLDLFKAIGLGLVVLLPLANPLTTVALFLGLAGNMNSAERNRQS
                     LMASVYVFAIMMVAYYAGQLVMDTFGISIPGLRIAGGLIVAFIGFRMLFPQQKAIDSP
                     EAKSKSEELEDEPSANIAFVPLAMPSTAGPGTIAMIISSASTVRQSSTFADWVLMVAP
                     PLIFFLVAVILWGSLRSSGAIMRLVGKGGIEAISRLMGFLLVCMGVQFIINGILEIIK
                     TYH"
     gene            14020..14454
                     /gene="marR"
                     /locus_tag="J1Y83_002900"
     CDS             14020..14454
                     /gene="marR"
                     /locus_tag="J1Y83_002900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310164.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple antibiotic resistance transcriptional
                     regulator MarR"
                     /protein_id="HBA7631031.1"
                     /translation="MKSTSDLFNEIIPLGRLIHMVNQKKDRLLNEYLSPLDITAAQFK
                     VLCSIRCAACITPVELKKVLSVDLGALTRMLDRLVCKGWVERLPNPNDKRGVLVKLTT
                     SGAAICEQCHQLVGQDLHQELTKNLTADEVATLEHLLKKVLP"
     gene            14475..14858
                     /gene="marA"
                     /locus_tag="J1Y83_002901"
     CDS             14475..14858
                     /gene="marA"
                     /locus_tag="J1Y83_002901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MDR efflux pump AcrAB transcriptional activator
                     MarA"
                     /protein_id="HBA7631032.1"
                     /translation="MSRRNTDAITIHSILDWIEDNLESPLSLEKVSERSGYSKWHLQR
                     MFKKETGHSLGQYIRSRKMTEIAQKLKESNEPILYLAERYGFESQQTLTRTFKNYFDV
                     PPHKYRMTNMQGESRFLHPLNHYNN"
     gene            14890..15108
                     /gene="marB"
                     /locus_tag="J1Y83_002902"
     CDS             14890..15108
                     /gene="marB"
                     /locus_tag="J1Y83_002902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple antibiotic resistance protein MarB"
                     /protein_id="HBA7631033.1"
                     /translation="MKPLLSAIATALILFSAQGVAEQTTQPVVTSCGNVVVVPPSQEQ
                     PPFDLNHMGTGSDKSDALGVPYYNQHAM"
     gene            complement(15139..16038)
                     /gene="eamA"
                     /locus_tag="J1Y83_002903"
     CDS             complement(15139..16038)
                     /gene="eamA"
                     /locus_tag="J1Y83_002903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416050.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylserine/cysteine exporter"
                     /protein_id="HBA7631034.1"
                     /translation="MSRKDGVLALLVVVVWGLNFVVIKVGLHNMPPLMLAGLRFMLVA
                     FPAIFFVARPKVPLNLLLGYGLTISFAQFAFLFCAINFGMPAGLASLVLQAQAFFTIV
                     LGAFTFGERLHGKQLAGIALAIFGVLVLIEDSLNGQHVAMLGFMLTLAAAFSWACGNI
                     FNKKIMSHATRPAVMSLVIWSALIPIIPFFVASLILDGSATMIHSLVTIDMTTILSLM
                     YLAFVATIVGYGIWGTLLGRYETWRVAPLSLLVPVVGLASAALLLDERLTGLQFFGAV
                     LIMTGLYINVFGLRWRKAVKVGS"
     gene            16233..17420
                     /gene="ydeE"
                     /locus_tag="J1Y83_002904"
     CDS             16233..17420
                     /gene="ydeE"
                     /locus_tag="J1Y83_002904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux MFS transporter YdeE"
                     /protein_id="HBA7631035.1"
                     /translation="MNLSLRRSTSALLASSLLLTIGRGATLPFMTIYLSRQYSLSVDL
                     IGYAMTIALTIGVVFSLGFGILADKFDKKRYMLLAITAFASGFIAIPLVNNVTLVVLF
                     FALINCAYSVFATVLKAWFADNLSSTSKTKIFSINYTMLNIGWTIGPPLGTLLVMQSI
                     NLPFWLAAICSAFPMLFIQIWVKRGEKIIATETGSVWSPKVLLQDKALLWFTCSGFLA
                     SFVSGAFASCISQYVMVIADGDFAEKVVAVVLPVNAAMVVTLQYSVGRRLNPANIRAL
                     MTAGTLCFVIGLVGFIFSGNSLLLWGMSAAVFTVGEIIYAPGEYMLIDHIAPPGMKAS
                     YFSAQSLGWLGAAINPLVSGIVLTSLPPFSLFIILALVIVVAWVLMLKGIRARPWGQP
                     ALC"
     gene            17547..17642
                     /gene="mgtS"
                     /locus_tag="J1Y83_002905"
     CDS             17547..17642
                     /gene="mgtS"
                     /locus_tag="J1Y83_002905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein MgtS"
                     /protein_id="HBA7631036.1"
                     /translation="MLGNMNVFMAVLGIILFSGFLAAYFSHKWDD"
     gene            complement(17868..18752)
                     /gene="dgcZ"
                     /locus_tag="J1Y83_002906"
     CDS             complement(17868..18752)
                     /gene="dgcZ"
                     /locus_tag="J1Y83_002906"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcZ"
                     /protein_id="HBA7631037.1"
                     /translation="MIKKTTEIDAILLNLNKAIDAHYQWLVSMFHSVVARDASKPEIT
                     DNHSYGLCQFGRWIDHLGPLDNDELPYVRLMDSAHQHMHNCGRELMLAIVENHWQDAH
                     FDAFQEGLLSFTAALTDYKIYLLTVRSNMDVLTGLPGRRVLDESFDHQLRNAEPLNLY
                     LMLLDIDRFKLVNDTYGHLIGDVVLRTLATYLASWTRDYETVYRYGGEEFIIIVKATN
                     DEEACRAGVRICQLVDNHAITHSEGHINITVTAGVSRAFPEEPLDVVIGRADRAMYEG
                     KQTGRNRCMFIDEQNVIN"
BASE COUNT         4911 a         4766 c         4355 g         4789 t
ORIGIN      
        1 tgagaatata gtttccaggg ttattacatt tcacgaagaa cgataatatc tttaaattac
       61 aaggacacct aatcacaaca agtttattgc agcacttgtt gtagagagta tttgcaaaaa
      121 cattttatgt cgctaaataa catgacgatt tcattatgca tgatatttat aaacagcgac
      181 taaacagaat attattgcga acaagggaag tgtcaggaaa ggtcaagagg tactaatagt
      241 ttatatggct ataaacatta gctatggtta atatttgcct ggtctaacta acacggaatt
      301 gctctaaaca gataagtgca gggtttatta aagctgaccg acagactttc ttaacctggt
      361 aatatctctc ttaggtgact ctccaaacat ccgcgaatat tcccgactga aatgggataa
      421 gctttcgtaa ccgacagcat atgctgcagt ggtgacatcg taatgttcat tcagcatcag
      481 tcgcctggct tcattgaggc gtagccattt ctgataccgg agtggactca ttcccgcaag
      541 ctgtctgaag tgttgatgga aagagggggt gttcatttgc acgcggtttg ccagcctgac
      601 tcttcaacgc tgagaagatg cggcaaacgg caaacctgca taccagccag cctatatccg
      661 gtatgcacag gccaattatg aaagaggaga ggctttatgt tctggaaccc tgaagtaata
      721 acgacgaccg gaaaatcgct tcgcatcact caaattcaat ttaacataat atacattatg
      781 cgcaccaaca taaaccaagg gagaatccag tcgttggcgg ttcatggatt gttctgctca
      841 ttaacaacga ccaaaacccc ataccttact taataccgta ttctcggctt aaccgaccgt
      901 gcaacctcaa cacattggtt tcaactgccg tcaccacgtt ctccggaaaa tctgtcggta
      961 atgaagtctt cacgttatcc agtgctgccg gaatcattct ggcaaagtca ctcaggattt
     1021 catgcatctg cacttccggg aatttcagca cctttgctgt cgccagaaaa tgtcgcggat
     1081 aaattttatc gattgccgtt tttttgcctt tggatgcgtt aagccccatt gccagtttga
     1141 gatcgctgat gtgtattccc gtaccgccaa ggaccggaaa tgccgaaatg atgtcataaa
     1201 atggtgtgag ccgataactg ccgccagcct gaataaatac ggagaagttt tttgcatggc
     1261 catccgttgc gccaatcaac cactggaaga cctgaaactt cataaaatca tagcgatctt
     1321 tcagcgcctc gctggagccc atcaaaaaag ccataatttg cgcgataccg gggcctccat
     1381 ctgattcata tttcaccgat gaaggtaaac cgaatgtctg gcacatatcc tcctgtggca
     1441 agcgaagtaa aaccgttcgc tcagtattcc aacgcctgtc aaaacgttcg accgctaacg
     1501 cgcgcaccct tcccgcttta atgatttctg cgtccggaac attcaacccg agttctttcg
     1561 ccagcagcaa acagtaatac tcattatcaa cgctttggct aagatcgagc gtcgcattgg
     1621 gctgtctgat ttcgccaatc ggcaatttga taatgtgcgt cgttggcgtt attcccttcg
     1681 gaatgcacca gtcattgcct attctgagca gcgcagtctt ctcctgtgcg ccagcaaccg
     1741 agatgcgaaa gtcattttct tctctaatca tgcctaacgg gatatctgct ttataagccg
     1801 ttaatacttc ttcaagtctg gcttcagtaa gcttttccca tgccattatc ggacacgtta
     1861 cggtttcatc ttcggggatt aacgtcacgg caccaacgct gtctcgccct atttctgaca
     1921 ataaatcaaa cggttgtctg gatttagcat gataacgttt aacgatccgg tcacgtacaa
     1981 tcgggctatc gggtaacagg ttatcgaaga agttaaatac ggcatcagag gtgatattcc
     2041 ccctctgcaa tggcagcgaa agtgacaacg gtctggcata acggctggct aaccactcag
     2101 gtgcatactt aaaggtgtgc gcgccgttgg ctaacttcgt taactcgcct acccgctggt
     2161 tgttcatcca agtgactagt ttaggcatca ccactccaga tcttgctgtt ctgttgcttc
     2221 tggcgaggca ttttttgcgt cgcatagcgt cattgagagt tcaagcgact gtaaaatctt
     2281 aaaaaatgtc gtgagcgagg tattgtcagg attgttttcg aaattggaaa ttgtcgcctg
     2341 cttaatgccg atttttttcg ccaactcgct ctgcgtccag ccgttttgct ggcgaaccag
     2401 tttcattgca ttcgccaatt gcattgggct atagatcttc tgaaagctca tcatgtcata
     2461 cgtccacagc aaatttatcc gcttaagggg atattatcag ttttatcctt tagtgaggat
     2521 aaagtcaatc tggaaacaca tcttatccgc tctacgggat aaacgctgcg atatccgcga
     2581 tcgcggataa aaaggaggat ttaaaaaagc acatcctaat gcggtggctt ctttgactac
     2641 cggaatctgt aatcttaatg acctggccgc gtaaaaaacg tctgttcaat ggggtgatgc
     2701 cgtgaggcga atttatcagt tttatctaca attggggtaa cgcgctgacg ggagtaaaaa
     2761 aatgtctgac tggaacccct ctttatatct gcactttgcc gctgaacgat cgcgtccggc
     2821 agtggagctg cttgccaggg tgccgctgga gaatatcgaa tacattgccg atcttggctg
     2881 tggcccaggt aacagcaccg cccttctaca ccaacgttgg cctgctgcca ggataacggg
     2941 catcgactca tctccggcga tgattgctga agcgcgcagt gctttgccag actgcctgtt
     3001 tgtggaagca gatatccgca actggcaacc ggaacaggca ctcgatctga tttttgctaa
     3061 tgcctcgctg caatggctgc ccgaccacta cgaattgttt cctcatttgg tttcgttact
     3121 cagtccgctg ggcgtgctgg cagtacagat gccagataac tggctggagc cgacccatgt
     3181 gctcatgcgc gaagttgcct gggaacaaaa ctacccggat cgcgggcgtg agccgctggc
     3241 tggcgttcat gcttactacg atattctgag tgaagccgga tgtgaggtcg atatctggcg
     3301 aaccacttac tatcaccaga tgccgtcgca ccaggcgatt atcgattggg tgaccgccac
     3361 tggattacgt ccgtggttac aggatctgac cgagagcgaa cagcagcatt ttcttacgcg
     3421 ctaccatcag atgctggaag agcaatatcc cctccaggag aacggacaga tattgctggc
     3481 atttccgcgt ctgtttattg ttgcccgccg tacggagtaa ttatcacgtc agctggtaat
     3541 ggcgatcggg aagaattttc gctggaattt ctgcttcatc gttcatctgt aacaggtcaa
     3601 tttcaatagc gttgcagatg gcatccagcg gtaaatcatt gttttcagta ccgaagggat
     3661 cttccagttc ttccgccaga caatccagcg aaataaaagt gtaggaaatc agcacagaga
     3721 taaaaggcgt catgtaatgc aggtccacga ccagcgcaaa cggcagcatg atacaaaaca
     3781 gatacacggt gcgatgcaaa atcagggtgt aggcaaaggg aattggcgta taggcaatgc
     3841 gctcgcatcc tgccaggacc gctgaaatat cattaagccg atcgttgagg ctaataaaca
     3901 ggatatctga aagttgtcca ttgcgtcgct gaaccgccaa ccactccccc attattaaca
     3961 agatgcggtt agctggagag ttagaagcca gtacacgctg aagatcttca gtcttgagat
     4021 aatgagccag cacttccgcc tgtggctgtt tgcgtaatgt catgcgtaaa cagtgggcga
     4081 aggcgatttg cagccgggca aactccctta cacttgccga atccggcaat gtcgttttta
     4141 cctcgcgcag taacgaccgc gaggcaatca tcaactgccc ccaaagtttt cgcgcttcaa
     4201 cgtaacgggc gtaaccggca ttattacgaa aaccaagaaa aatggcgatg gcgacaccga
     4261 gaatgctgaa cggtgcgagg gtgaatttaa tgcccaaatg cgtgtaccag ggcagcatga
     4321 aaataacagc gatagaaaag agaaaattga gtagtaagcg cgaggatatc ttggataata
     4381 ctgaaccgtg ccagacaaaa atacggcgca gccagtgttg ttgtggacga acaatcatgg
     4441 ttatcttcag gcgtggaaaa gtcgccctat taaacgtgat tacgattaca ttctcaagac
     4501 gctacttaca aattacctac aagcttacaa cattaacagg tgctgcatgt ggcatctgtt
     4561 aatgataaag gcacatagaa tgttgtaact aatatagcga aataagaaac ccggtcgaaa
     4621 ccgggttcag agtattagca caacgggcgt acagcttcgc gcatcccttt ttcgagaatc
     4681 gcatccaggt cattagcaac ctgctcgacc agaccaggca cttgcgtcag gtcctgctcc
     4741 cagtggtctt tttctgccaa cacaatcgct accagttcct gggtcccgat aacacggtca
     4801 cgatactggc tccagagttg ctgataacgt tccagccagt gtgcatcgtc ctgtaccgga
     4861 tatgtttctc cattacgctc accgcgatag aacgcaatca atgccgctaa tgcgaaagta
     4921 aggcgcgccg gaagtgtgcc gtttgccttc tgccctgcca gcagctgcgg caggatgcgt
     4981 gtgcggaact tggtcatacc gttgagcgcg atagacagca gttgatgctt gatgtacggg
     5041 ttacggaaac gtccggtgac tgcactggcg aaagactcca gctcatcgcg tggcaaatcc
     5101 agtaccggaa taatttcttc gtagatggct ttttcaacga atgcgcaaat ttcagcatcg
     5161 ttcatcgctt cacctacggt atccagccct gcctggaacg ccaccggcac cagcgcggtg
     5221 tgcgcaccgt tgaggattgc cactttgcgt tctttatacg gtttaatatc gtcaacgatc
     5281 agcacgttga gcggatattt gtccagacga agttcagtcg ctaaggattt cggcccctga
     5341 atcacaaaca ggtaaaagtg ttcagcggtg tcgaggaaac catcgtggta gcccagttct
     5401 tcttccagtt tagccacttc atcacgcgga taaccggtaa cgatacggtc caccagcgta
     5461 gaacagaagc tgttagcctg atccagccat tgaataaatg cttctggcag tgcccactct
     5521 tgcgcatagc gcagcaccag ttcacgcaac gcgtcgccat tatagtcaat caactcacac
     5581 gggatgatga tccaaccttt atccagcgca ccgttgaaat ggctaaaacg ttcgaacagc
     5641 agacgggtca gttttgccgg ataacttacc gctggcgcgt catcgaattt atcgcccgca
     5701 tggtagctga tacccgcttc ggtggtgtta gagaaaacaa agcgcatttc cgggttgtga
     5761 gccagtttca ggaattcatc gtattcactg tagacgctga tttcacgatt aaccgagcga
     5821 atcagacgcg cgtcgctgac cgcttcaccc ttctcattca ggccacggat aatggtggta
     5881 tacagaccat cctgcgtgct cagtgacggc gggaatgaag tttcaatcgg acgaacaacg
     5941 accacgccag aattcagatc ggtgtgctca ttcaggagat cgatttgcca gtcaacaaag
     6001 gcgcgcagga agttaccttc accaaactga atgatacgtt ctggatactg tgcaccggga
     6061 aaatcgcgac gatttagtgt tttcacaatg ggttcccttc tgattagtca tacaacctgt
     6121 ttaaattggt acgacaggtt agcaaacttt aatacgccga acccctgttt tgatcaactc
     6181 ctgatgatta atgagcagtt ttatgagaaa agtgtggcgc ggatcatggt ttaatcgagg
     6241 aaacaggcct tttcttggat cataaagtgg tagaacacat tgcatttaat tcgctctgag
     6301 taaataaaga tgtcagacaa cttcctcacc gtaacgcata gtgctggtac ggttgcgccc
     6361 atctttcttc gagcgataca gacacgtatc ggcttcgacc atcaatttat taaagtcatc
     6421 ggtcagcgtt cggtacgatg cgcaaccact accgacgcca atacttaccg tgagataaag
     6481 cgttttttgt tgccaggtga acggttgaag ttctacgcct ttacggattt tttccgccat
     6541 tagcaggcca tcgacaggat tcaccgacgg cactgcaaca gcaaattctt cgccgcccat
     6601 tcgtgccacc agccccttat caccgacaat cttctgaata tgctgggcaa acacgcttaa
     6661 cactttatcg ccacattcat gtccatagtt atcgttaatg cttttgaaat agtcgatatc
     6721 aagcagcatg accgtcagat gttgtgtctg tttcagcgat ggacttttca gcgcctcata
     6781 aagaccggac cgtgagtaaa cctgggtcag aaaatcaaag tcggctcgca gcgcaacttg
     6841 cttcattagc gaattgatcg ctgccacgct aaaggaaacc ataattgggc atatcgccat
     6901 cgtggcaata ccgagacgtg cggagaacat ttgcggaatg gagaacggcg aaccgaccga
     6961 gatatcaatc accgaatttg ccaccagtac gacttccaca gcaccggtga caaaggtcaa
     7021 cagacatgtt acctgcggcg tatagcgcac tgcacaccag attaatgcag ggagcggaaa
     7081 cgccagactc cccgctccgc caatgactac cgaggctata accgacacaa taagcgcgat
     7141 agcaggcatc atctgctctg ctttaaagcg cggcagcact ccaggaattg ccaacgtcag
     7201 catacaaggc acgatcaaca cgcccgttga gaattgctca ctaaaccagt cggcaagcaa
     7261 aggccagaaa tccagactat caatactgac cgaaccaatc gccccgacaa tagcgcataa
     7321 taatgcggca agcagacagt aattaaatag ccgtaaggca cttactggct catacttatt
     7381 ttttcccaga cgcttatccc gtgcgaccag taaggcgacg gtaacaataa acatcatatt
     7441 ggagaaattg atagccagtg aaaccagccc ccattcggtg gttatggcat cataaaccag
     7501 catcgccaca taactgatcg catagtaatg cagtcgatta agccagacat agcgggcaaa
     7561 cacccccgcc attacgccat tcagcggcca gaaaagagaa agttcgtgaa ccagccgtaa
     7621 ctctgcacca ataaaataga acagcgtggt tagcacaaaa atagcgatgc tattacgtag
     7681 cagatgacct tcctggaaca aacggaaagt tgaaatgggc tgtacatgca ttaattatct
     7741 gtccatacaa ttgcgggtgg caattatctc aacgcaataa gtaaattctt agaaaggata
     7801 gcatgttcgc tgacaacggc ggcaactaaa gatacctaat gaatgccatt ttatcgattt
     7861 ccgtaattat catggctgat tcatctgtat aaccagccag tggtagatta ccgtcacaat
     7921 ataacgttgt tgctcctcac ttaacgcttc cccctgagga atgttatgcc acttagccag
     7981 acaaccgcga caacaggtcg cggtggcgtg ttgagcgata aaaaccggat gcccgcgcat
     8041 gggggtttgc ttaccgtcgt tagccggtaa tgccggagca agacgtttag cgacaaaatc
     8101 cgccgcatgt tgttcaatga ctggcgcgcc tttctccaga caatactgac gctctttcac
     8161 acccaaacga aagcgagagc gaaattttga acgggataaa cgcgcgaaga gtggatcgag
     8221 agactgcatt aataaactga acgccctaat tgtttcgtca attgttcaag gacggcaatg
     8281 cccgcaagcg agttaccggc atcatccagt tccggactcc agactgcgat ggccatttca
     8341 tgcggaacaa tcgccacaat accgccacca acgccagatt ttgccggtag tcccacccgc
     8401 caggcaaact cccccgcgtt ctggtacata ccgctggtcg ccatcagcgc gttaatttgc
     8461 cgcgcctgca ttggcgtcac cacaggttca tcaatatgaa tagctttccc ctgattcgcc
     8521 agaaagacaa acgtccgggc cagctctaca cagctcattt tcagagcgca gtaatgaaag
     8581 tagttttgca gaacggttgt cacgtcatga tggaaattgc caaacgactt catcagccag
     8641 gcgatagccg catttcgcgc ggaatgttca aattcggaac gcgctaccac cgtatcgtag
     8701 gaaatatcag acacaccact taagccgcgc acgacttcca gcatacgttg ccgaggagcg
     8761 cttaatcgcc cttgcaacat atcgcagacc accagcgcac cggcattaat aaacggatta
     8821 cgcggaatac cctgctccat ttccagttgc actaaagaat tgaacggtga tccagacgga
     8881 tctttgccga cgcgttgcca gatttcctct tcggagtaat gacgcatggc gacaacgaga
     8941 ctcagcactt tggaaataga ctgaatggaa aaacgttctt gcgcgtctcc ggcctgaaaa
     9001 agctgtccgt caacggtaca gatagcaatc cccaatcggg aaccgtctac cgtagccagc
     9061 gccggaatat aatccgcgac tttaccctga ccaatgagcg gccgcacttg ccgcaagatg
     9121 ttttctaaaa ttgcattatc catggcgact gccactttct actcctggac cgcaggtctg
     9181 aaaagacctg cgagtatatc agagctgaat atatagcgtc agacccggtc tttccacacc
     9241 gtctggatat tacagaattc gtgtaagccg aaatgggaaa gctcacgacc aaagccactc
     9301 ttcttcacgc caccaaaggc gactcgcgca tcgctggcac aataaccatt gataaacacc
     9361 ccaccgcatt ccagacgtgc cgccatctgt ctggcctgtg tttcgtcggt ggtgaaaatg
     9421 gtcgctgaaa ggccgaactc actatcatta gccagttcca gcgcatgttc tgcatctttc
     9481 gcaatggtga ttgccgcaac ggggccaaac atttcttccc gaaacgcggt catttctggg
     9541 gtaacattcg ccagaaccgt tggcggatag tagttacctg ccccagccat cttttcccct
     9601 cccagtaaca aacgcgcacc ctgcgccagt gttttttcca cctgatgatg cagctcatca
     9661 cgtaaatcaa aacgagccat tggtccaaga gcgttctctt catcacgggg atcgcccatt
     9721 ttcaaggctg ccgcagctgc cacaaaacgt tcggtaaatg ccgaagcaat tccctcttcg
     9781 atgataaagc gttttgccgc tgcacaaacc tgtccggtat tctgataacg tccggctacc
     9841 gccgctttca ccgccagttc cagatcggca tcgttaagca caataaacgg atccgaacca
     9901 cccagttcca gtacgcattt tttcagtgcc gctccagcct gtgcgccaat agccgctcca
     9961 gcacgaacac ttccggtcac tgtgacagca gcaatgcgcg agtctttaat catcagactg
    10021 acaccgtcgt tgtcggcatt cagccagcca tatacgcctt gtgggatacc cgcatcttta
    10081 aacacctggg caatgagctg tgcacagccc atgacattcg gcgcatgttt aagtaagtag
    10141 ccgttaccag caagaatgat gggaacagcg ccacgcatca cctgccataa cggaaaattc
    10201 cacggcataa tcgccagaat cgtccccaac gctcgatact caataaccgc ctgctgattt
    10261 tccaccagcg taggttctgc cttcagcatt gccggcccat gttctgcata ccagtcacac
    10321 aaattcgccg atttcgccac ttcagcgcgc gcctggttga ttggtttgcc catttcacgg
    10381 gtgatcattt gcgccatttc ttcgctacgg gcgcgcagag ccttaccgat atcacgcagt
    10441 ttttcagcac gataatctat atttgcctcg cgccagtcgc gaaagcctgc tgctgccagc
    10501 tgaagcgcgt tttcgatatc gtcaacacca gcccacggca gcacagaaag ttgttcacca
    10561 gtggcaggat ttatcgaaat tgcatgagtt gccggagtaa tggtcatcgg ggtatctcct
    10621 ttatgagtca tggtatgaag atacgcagat ttactcttgc tttaaaatga ataatattaa
    10681 gccacttatt cacgaatcga gaatgttatg gatctgaccc aactggagat gttcaacgcg
    10741 gttgccgaag ctggcagcat tacccaggct gcagcaaaag tgcatcgcgt gccgtcgaat
    10801 ctcactaccc gtctacgtca gctggaaaca gaactagggg ttgatctgtt tattcgcgag
    10861 aatcagcgtt tacgtctctc gcctgccggg cataactttt tacgctatag ccaacaaatt
    10921 ctcacgttag tggatgaagc gcggagcgtt gtcgctggcg atgagccgca aggtttattt
    10981 tctctgggat cgctggaaag caccgctgca gtgcgcattc cggccacgct ggcggaattt
    11041 aaccgtcgtt atcccaaaat tcagttttca ctttccaccg gcccttccgg cactatgctg
    11101 gaaggtgtgc tggagggaaa actgaatgcg gcatttattg atgggcccat taaccatact
    11161 gccattgacg ggataccggt ataccgcgag gaactgatga tcgtcacgcc acaaggacat
    11221 gcgccagtaa cccgtgccag tcaggtgaat ggcagtaaca tttatgcctt ccgtgccaat
    11281 tgttcgtatc gtcgccactt cgaaagctgg tttcatgctg acggtgccgc tccgggaact
    11341 attcacgaga tggagtctta tcacgggatg ttggcctgtg tggtcgcagg agcaggcatt
    11401 gcgcttattc cacgctcaat gctggaaagt atgccggggc atcaccaggt tgaagcgtgg
    11461 ccgttagctg agcaatggcg ttggttaaca acctggctgg tctggcgtcg tggtgcgaaa
    11521 acgcgtccgc ttgaggcatt tattcaactg ctggatgcgc ctgactcggc aaaacagggt
    11581 taccaatgag ctatttttga tagttcatac ggctaatatg ctctatatag tgatgttccg
    11641 atgacttatg actatatggg gcaaatatgg ttacgccagt aagcatcagc aattacatat
    11701 ctcttcctga tgattttccc gttcgtaata ttgcacctca agtaaaagaa gaacaggccc
    11761 ccttcctgat cgtcattaaa atgtgatgtt aacaactctc tgatgcgtga cagaacaacc
    11821 gtcagagaga tggaaatgcg atccagatca caaatgcatt gtattcacat cattaaccgt
    11881 tttaagatca tttcatcact ttttcgcaac tcacccgata atctgttatg acaacaaaca
    11941 ctgtttcccg caaagtagcg tggctacggg tcgttacgct ggcagtcgcc gcctttatct
    12001 tcaataccac cgaatttgtc cctgttggcc tgctctctga cattgcgcat agttttcaca
    12061 tgcaaaccgc tcaggtcggc atcatgttga caatttatgc atgggtagtg gcgctaatgt
    12121 cattgccttt tatgttaatg accagccagg ttgaacgccg caaattactg atctgcctgt
    12181 ttgtggtgtt tattgccagc cacgtactgt cgtttttatc gtggagtttt accgttctgg
    12241 tgatcagtcg cattggtgtg gcttttgcac atgcgatttt ctggtcgatt acggcgtctc
    12301 tggcgatccg tatggctccg gccgggaagc gagcacaggc attgagttta attgccaccg
    12361 gtacagcact ggcaatggta ttaggtttac ctctcgggcg cattgtgggg cagtattttg
    12421 gctggcgaat gaccttcttc gcgattggta tcggggcgct tattaccctt ttgtgcctga
    12481 ttaagttact tcccttgctg cccagtgagc attccggttc attgaaaagc ctcccgctat
    12541 tgttccgccg cccggcattg atgagcattt atttgttaac tgtggtggtt gtcaccgccc
    12601 attacacggc atacagctat atcgagcctt ttgtacaaaa cattgcggga ttcagcgcca
    12661 actttgccac ggcattactg ttattactcg gtggtgcggg cattattggc agcgtgattt
    12721 tcggtaaact gggtaatcag tatgcgtctg cgttggtgag tacagcgatt gcgctattgc
    12781 tggtgtgcct ggcactgctg ctacctgcgg cgaacagtga aatacacctc gggatgctga
    12841 gtattttctg ggggatcgcg atgatgatca tcgggcttgg tatgcaggtt aaagtgctgg
    12901 cgctggcacc agatgccacc gacgtcgcga tggcgctatt ctccgggata tttaatattg
    12961 gaatcggcgc gggtgcgttg gtaggtaatc aggtgagtct gcactggtca atgtcgatga
    13021 ttggttatgt aggcgcggtg cctgcttttg ctgcgttaat atggtcaatc atcatttttc
    13081 gccgctggcc agtgacactc gaagaacaga cgcaatagtc gaaaggccca ttcgggcctt
    13141 ttttaatgat acgttttaat gatttccagg atgccgttaa taataaactg cacgcccata
    13201 cataccagca ggaatcccat cagacgggag atcgcttcaa tacccccctt gcccaccagc
    13261 cgcataattg cgccagagct gcgtagactt ccccacaaaa taaccgccac caggaaaaag
    13321 atcagcggcg gcgcaaccat cagtacccaa tcagcgaagg ttgaactctg acgcactgtg
    13381 gaggccgagc taataatcat cgcaatggtt cccggaccgg cggtacttgg cattgccagt
    13441 ggtacaaaag caatattggc actgggttca tcttccagct cttccgactt gcttttcgct
    13501 tccggtgaat caatcgcttt ctgttgcgga aagagcatcc gaaaaccgat aaacgcgacg
    13561 atcaaaccgc ctgcaattcg cagaccggga atcgaaatgc caaatgtatc catcaccagt
    13621 tgcccggcgt aatatgccac catcatgatg gcaaatacgt acaccgaggc catcaacgac
    13681 tgacgattac gttcggcact gttcatgttg cctgccaggc caagaaataa cgcgacagtt
    13741 gttaatgggt tagctaacgg cagtaacacc accagcccca ggcctattgc tttaaacaaa
    13801 tctaacattg gtggttgtta tcctgtgtat ctggtttatc agcgaaaagt ataaggggta
    13861 aacaaggata aagtgtcact ctttagctaa ccttgcatcg cattgaccaa aacttgaacc
    13921 gatttagcaa aacgtggcat cggtcaattc attcatttga cttatacttg cctgggcaat
    13981 attatcccct gcaactaatt acttgccagg gcaactaatg tgaaaagtac cagcgatctg
    14041 ttcaatgaaa ttattccatt gggtcgctta atccatatgg ttaatcagaa gaaagatcgc
    14101 ctgcttaacg agtatctgtc tccgctggat attaccgcgg cacagtttaa ggtgctctgc
    14161 tctatccgct gcgcggcgtg tattactccg gttgaactga aaaaagtgtt gtcggtcgac
    14221 ctgggggcac tgacccgtat gctggatcgc ctggtctgta aaggctgggt ggaaaggttg
    14281 ccgaacccga atgataagcg cggcgtactg gtaaaactta ccaccagcgg cgcggcaata
    14341 tgtgaacaat gccatcaatt agttggccag gacctgcatc aagaattaac aaaaaacctg
    14401 acggcggacg aagtggcaac acttgagcat ttgcttaaga aagtcctgcc gtaaacgaaa
    14461 aaagaggtat gacgatgtcc agacgcaata ctgacgctat taccattcat agcattttgg
    14521 actggatcga ggacaacctg gaatcgccac tgtcactgga gaaagtgtca gagcgttcgg
    14581 gttactccaa atggcacctg caacggatgt ttaaaaaaga aaccggtcat tcattaggcc
    14641 aatacatccg tagccgtaag atgacggaaa tcgcgcaaaa gctgaaggaa agtaacgagc
    14701 cgatactcta tctggcagaa cgatatggct tcgagtccca acaaactctg acccgaacct
    14761 tcaaaaatta ctttgatgtt ccgccgcata aataccggat gaccaatatg cagggtgaat
    14821 cgcgcttttt acatccatta aatcattaca acaactagtt gataacgtga caacgtcact
    14881 gaggcaatca tgaaaccact tttatccgca atagcaactg cgcttattct cttttctgcg
    14941 cagggcgttg cggaacaaac cacgcagccg gttgttactt cctgtggcaa tgtcgtggtt
    15001 gttcccccat cgcaggaaca accaccgttt gatttaaatc acatgggtac tggcagtgat
    15061 aagtcggatg cgctcggcgt gccctattat aatcaacacg ctatgtagtt tgttctggcc
    15121 ccgacatctc ggggcttatt aacttcccac ttttaccgct ttacgccacc gcaagccaaa
    15181 tacattgata tacagcccgg tcataatgag caccgcaccg aaaaattgca gacccgttaa
    15241 acgttcatcc aacaatagtg ccgcacttgc cagtccaact acaggcacca gtaacgataa
    15301 cggtgcaacc cgccaggttt catagcgtcc aagcagtgtt ccccagatcc cataaccaac
    15361 aattgtcgcc acaaacgcca gatacatcag agacaagatg gtggtcatat cgatagtaac
    15421 cagactgtga atcatggttg cggaaccatc gagaatcagc gaggcaacaa agaagggaat
    15481 gattgggatt aatgcgctcc agattaccag cgacatcacc gccggacgcg ttgcgtgcga
    15541 catgatcttt ttattgaaga tgttgccaca cgcccaacta aatgctgccg ccagggtcaa
    15601 cataaagccg agcatcgcca catgctgacc gttcagacta tcttcgatta acaccagtac
    15661 gccaaaaatc gctaaggcga tccccgccaa ttgtttgcca tgcagtcgct caccgaaagt
    15721 aaacgcgcca agcacgatag taaaaaacgc ctgtgcctgt aacaccagcg aagccagtcc
    15781 agcaggcata ccaaagttaa tggcgcagaa cagaaaagca aactgcgcaa aactgatggt
    15841 taatccatac cccagcagca aattcagtgg tactttcggt cgtgcgacaa aaaagatggc
    15901 cgggaaagcg accagcataa agcgcaaacc ggccagcatc agcggtggca tgttatgaag
    15961 ccccactttg atgaccacaa aatttagccc ccatacaacc actaccagta gcgccaacac
    16021 cccatctttt cgcgacattc tcccgcctct gaatttcatc ttttgtaagc aatcaactta
    16081 gctgaattta cttttcttta acagttgatt cgttagtcac cgtttacgac ggcattaatg
    16141 cgcaaataag tcgctatact tcggattttt gccatgctat tcctttacat ctctaaaaca
    16201 aaacataacg aaacgcactg ccggacagac aaatgaactt atccttacga cgctctacca
    16261 gcgcgcttct tgcctcgtcg ttgttattaa ccatcggacg cggcgctacg ctgccgttta
    16321 tgaccattta cttgagtcgc cagtacagcc tgagtgtcga tctaatcggt tatgcgatga
    16381 caattgcgct cactattggc gtcgttttta gcctcggttt tggtatcctg gcggataagt
    16441 tcgacaagaa acgctatatg ttactggcaa ttaccgcctt cgctagcggt tttattgcca
    16501 ttcctttagt gaataacgtg acgctggtgg tgctcttttt tgcccttatt aactgcgcct
    16561 actccgtttt tgctaccgtg ctgaaagcct ggtttgccga caatctttcg tccaccagca
    16621 aaacgaagat tttctcaatc aactacacca tgctcaacat tggctggacc atcggtccgc
    16681 cgctcggcac gctgttggtg atgcagagca tcaatctgcc cttctggctg gcagctatct
    16741 gttccgcgtt tcccatgctt ttcattcaaa tttgggtaaa gcgcggcgag aaaatcatcg
    16801 ccacggaaac aggcagtgtc tggtcgccga aagttttatt acaagataaa gcactgttgt
    16861 ggtttacctg ctctggcttt ctggcttctt ttgttagcgg cgcatttgct tcatgcattt
    16921 cacaatatgt gatggtgatt gctgatggtg attttgccga aaaggtggtc gcggttgttc
    16981 ttccggtgaa tgctgccatg gtggttacgt tgcaatattc cgtgggccgc cgccttaacc
    17041 cggctaacat ccgcgcgctg atgacagcag gcaccctctg tttcgtcatc gggctggtcg
    17101 gttttatttt ttccggcaac agcctgctat tgtggggtat gtcagctgcg gtatttactg
    17161 tcggtgaaat catttatgcg ccgggcgagt atatgttgat tgaccatatt gcgccgccag
    17221 gaatgaaagc cagctatttt tccgcccagt cactgggctg gcttggtgct gcgattaacc
    17281 cattagtgag tggcatagtg cttaccagtc tgccaccttt ttcgctgttt atcatcttag
    17341 cgttagtgat cgttgttgcg tgggtgctga tgttaaaagg aattcgtgca agaccgtggg
    17401 ggcagcccgc gctttgttga tttaagtcga acacaataag aatttaattc agccttcgtt
    17461 taggttacct ctgctaatat ctttctcatt gagatgaaaa ttaaggtaag cgaggaaaca
    17521 caccacacca taaacggagg caaataatgc tgggtaatat gaatgttttt atggccgtac
    17581 tgggaataat tttattttct ggttttctgg ccgcgtattt cagccacaaa tgggatgact
    17641 aatgaacgga gataatccct cacctaaccg gccccttgtt acagttgtgt acaaggggcc
    17701 tgatttttat gacggcgaaa aaaaaaccgc cagtaaaccg gcggtgaatg cttgcatgga
    17761 tagatttgtg ttttgctttt acgctaacag gcattttcct gcactgataa cggatcgttg
    17821 acacagtagc atcagttttc tcaatgaatg ttaaacggag cttaaactca gttaatcaca
    17881 ttttgttcgt caataaacat gcagcgattc cttccggttt gcttaccctc atacattgcc
    17941 ctgtccgctc ttccaatgac cacatccaga ggctcttcag gaaatgcacg actcacacca
    18001 gcagtcacgg taatgttgat atgcccttca gaatgtgtga tggcatggtt atcgactaac
    18061 tggcaaattc tgacacctgc acgacatgct tcttcatcat tagtcgcttt gacaataatg
    18121 ataaattctt cgcccccgta gcgataaacc gtttcgtaat cgcgcgtcca actggctaag
    18181 taagttgcga gggtgcgtaa tactacatcg ccgattaaat gcccgtaggt atcattaacc
    18241 aatttaaatc ggtctatatc caacaacatt aaataaagat tcagaggttc agcattgcgt
    18301 aactgatgat cgaaggattc atcaagaacc cgacgacccg gcaatcccgt cagaacatcc
    18361 atattgctac ggaccgtaag caaataaatt ttgtaatcgg ttaatgccgc agtaaaagaa
    18421 agcaaccctt cctgaaaagc gtcgaaatgc gcgtcctgcc agtgattttc aacaatagcc
    18481 agcattaatt cccgaccaca gttatgcata tgttgatggg cagaatccat tagccgaacg
    18541 taaggtaatt catcgttatc gagtggcccc agatgatcaa tccaccgacc aaactggcac
    18601 agcccataag aatggttatc cgttatttct ggcttactgg catctctcgc gaccacgctg
    18661 tgaaacatac ttaccagcca ctggtagtgg gcatcgatag ccttattgag atttaacaag
    18721 atggcatcaa tttccgttgt cttcttgatc attgccactc ctttttcaca gttccttgtg
    18781 cgcgctattc taacgagaga aaagcaaaat tacgtcaata t
//
LOCUS       DADSYM010000098        18622 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2415
            SAMN08039579-rid11723173.denovo.098, whole genome shotgun sequence.
ACCESSION   DADSYM010000098 DADSYM010000000
VERSION     DADSYM010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039579
            Sequence Read Archive: SRR6362533
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18622)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18622)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980162.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 03:39:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,484
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 4,347
            CDSs (with protein)               :: 4,347
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 43 of 85
            Pseudo Genes (incomplete)         :: 37 of 85
            Pseudo Genes (internal stop)      :: 19 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18622
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039579-rid11723173.denovo.098"
                     /strain="LGE2415"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8302 N 95.9628 W"
                     /collection_date="2015-08-24"
     gene            119..1258
                     /gene="arnB"
                     /locus_tag="J1Y83_002907"
     CDS             119..1258
                     /gene="arnB"
                     /locus_tag="J1Y83_002907"
                     /EC_number="2.6.1.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_024188371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-4-amino-4-deoxy-L-arabinose
                     aminotransferase"
                     /protein_id="HBA7631038.1"
                     /translation="MSGFLPFSRPAMGVEELAAVKEVLESGWITTGPKNQALEQAFCQ
                     LTGNQHAIAVSSATAGMHITLMALEIGKGDEVITPSLTWVSTLNMISLLGATPVMVDV
                     DCNTLMVTPEAIEAAITPRTKAIIPVHYAGAPADIDAIRAIGERYGIAVIEDAAHAVG
                     TYYKGRHIGAKGTAIFSFHAIKNITCAEGGLIVTDNENLARQLRMLKFHGLGVDAYDR
                     HTWGRAPQAEVLTPGYKYNLTDINAAIALTQLVKLEHLNTRRREIAQQYQQALAALPF
                     QPLSLPAWPHVHAWHLFIIRVDEQRCGISRDALMEALKERGIGTGLHFRAAHTQKYYR
                     ERFPTLSLPNTEWNSERICSLPLFPDMTTADADRVITALQQLAGQ"
     gene            1262..2230
                     /gene="arnC"
                     /locus_tag="J1Y83_002908"
     CDS             1262..2230
                     /gene="arnC"
                     /locus_tag="J1Y83_002908"
                     /EC_number="2.4.2.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate
                     4-deoxy-4-formamido-L-arabinose transferase"
                     /protein_id="HBA7631039.1"
                     /translation="MFEIHPVKKVSVVIPVYNEQESLPELIRRTTAACESLGKEYEIL
                     LIDDGSSDNSAHMLVEASQAEGSHIVSILLNRNYGQHSAIMAGFSHVTGDLIITLDAD
                     LQNPPEEIPRLVAKADEGYDVVGTVRQNRQDSWFRKTASKMINRLIQRTTGKAMGDYG
                     CMLRAYRRHIVDAMLHCHERSTFIPILANIFARRAIEIPVHHAEREFGESKYSFMRLI
                     NLMYDLVTCLTTTPLRMLSLLGSIIAIGGFSIAVLLVILRLTFGPQWAAEGVFMLFAV
                     LFTFIGAQFIGMGLLGEYIGRIYTDVRARPRYFVQQVIRPSSKENE"
     gene            2230..4212
                     /locus_tag="J1Y83_002909"
     CDS             2230..4212
                     /locus_tag="J1Y83_002909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional polymyxin resistance protein ArnA"
                     /protein_id="HBA7631040.1"
                     /translation="MKTVVFAYHDMGCLGIEALLSAGYEISAIFTHTDNPGEKAFYGS
                     VARLAAERGIPVYAPDNVNHPLWVERIAQLSPEVIFSFYYRHLICDEILQLAPRGAFN
                     LHGSLLPKYRGRAPLNWVLVNGETETGVTLHRMVKRADAGAIVAQLRIAIAPDDIAIT
                     LHHKLCHAARQLLEQTLPAIKHGNILEIAQRENEATCFGRRTPDDSFLEWHKPASVLH
                     NMVRAVADPWPGAFSYVGNQKFTVWSSRVHPHASKAQPGSVISVAPLLIACGDGALEI
                     VTGQAGDGITMQGSQLAQTLGLVQGSRLNSQPACAARRRTRVLILGVNGFIGNHLTER
                     LLREDHYEVYGLDIGSDAISRFLNHPHFHFVEGDISIHSEWIEYHVKKCDVVLPLVAI
                     ATPIEYTRNPLRVFELDFEENLRIIRYCVKYRKRIIFPSTSEVYGMCSDKYFDEDHSN
                     LIVGPVNKPRWIYSVSKQLLDRVIWAYGEKEGLQFTLFRPFNWMGPRLDNLNAARIGS
                     SRAITQLILNLVEGSPIKLIDGGKQKRCFTDIRDGIEALYRIIENAGNRCDGEIINIG
                     NPENEASIEELGEMLLASFEKHPLRHYFPPFAGFRVVESSSYYGKGYQDVEHRKPSIR
                     NARRCLDWEPKIDMQETIDETLDFFLRTVDLTDKPS"
     gene            4209..5099
                     /locus_tag="J1Y83_002910"
     CDS             4209..5099
                     /locus_tag="J1Y83_002910"
                     /EC_number="3.5.1.n3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000169712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-deoxy-4-formamido-L-arabinose-
                     phosphoundecaprenol deformylase"
                     /protein_id="HBA7631041.1"
                     /translation="MTKVGLRIDVDTFRGTREGVPRLLEILSKHNIQASIFFSVGPDN
                     MGRHLWRLVKPQFLWKMLRSNAASLYGWDILLAGTAWPGKEIGHANADIIREAAKHHE
                     VGLHAWDHHAWQAHSGNWDRQTMIDDIARGLRTLEEIIGQPVTCSAAAGWRADQQVIE
                     AKEAFHLRYNSDCRGAMPFRPLLESGTPGTAQIPVTLPTWDEVIGRDVKAEDFNGWLL
                     NRIQRDKGTPVYTIHAEVEGCAYQHNFVDLLKRAAQEGVTFCPLSELLSGTLPLGQVV
                     RGNIAGREGWLGCQQMAGSH"
     gene            5099..6751
                     /gene="arnT"
                     /locus_tag="J1Y83_002911"
     CDS             5099..6751
                     /gene="arnT"
                     /locus_tag="J1Y83_002911"
                     /EC_number="2.4.2.43"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A)
                     4-amino-4-deoxy-L-arabinosyltransferase"
                     /protein_id="HBA7631042.1"
                     /translation="MKSVRYLIGLFAFIACYYLLPISTRLLWQPDETRYAEISREMLA
                     SGDWIVPHLLGLRYFEKPIAGYWINSIGQWLFGANNFGVRAGVIFATLLTAALVTWFT
                     LRLWRDKRLALLATVIYLSLFIVYAIGTYAVLDPFIAFWLVAGMCSFWLAMQAQTWKG
                     KSAGFLLLGITCGMGVMTKGFLALAVPVLSVLPWVATQKRWKDLFIYGWLAVISCVLT
                     VLPWGLAIAQREPDFWHYFFWVEHIQRFALDDAQHRAPFWYYLPVVIAGSLPWLGLLP
                     GALYAGWKNRKHSATVYLLSWTIMPLLFFSVAKGKLPTYILSCFAPLAILMAHYALLA
                     AKNNPLALRINGWINIAFGVTGIIATFVVSPWGPMNTPVWQTFESYKVFCAWSIFSLW
                     AFFGWYTLTNVEKTWPFAALCPLGLALLVGFSIPDRVMEGKHPQFFVEMTQESLQPSR
                     YILTDSVGVAAGLAWSLQRDDIIMYRQTGELKYGLNYPDAKGRFVSGDEFANWLNQHR
                     QEGIITLVLSVDRDEDINSLAIPPADVIDRQERLVLIQYRPK"
     gene            6748..7083
                     /gene="arnE"
                     /locus_tag="J1Y83_002912"
     CDS             6748..7083
                     /gene="arnE"
                     /locus_tag="J1Y83_002912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-amino-4-deoxy-L-arabinose-phosphoundecaprenol
                     flippase subunit ArnE"
                     /protein_id="HBA7631043.1"
                     /translation="MIWLTLVFASLLSVAGQLCQKQATCFAAVNKRRKHIVLWLGLAL
                     ACLGLAMVLWLLVLQNVPVGIAYPMLSLNFVWVTLAAVKLWHEPVSLRHWCGVAFIIG
                     GIVILGSTV"
     gene            7083..7469
                     /gene="arnF"
                     /locus_tag="J1Y83_002913"
     CDS             7083..7469
                     /gene="arnF"
                     /locus_tag="J1Y83_002913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416761.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-amino-4-deoxy-L-arabinose-phosphoundecaprenol
                     flippase subunit ArnF"
                     /protein_id="HBA7631044.1"
                     /translation="MGLMWGLFSVIIASAAQLSLGFAASHLPPMTHLWDFIAALLAFG
                     LDARILLLGLLGYLLSVFCWYKTLHKLALSKAYALLSMSYVLVWIASMVLPGWEGTFS
                     LKALLGVACIMSGLMLIFLPTTKQRY"
     gene            complement(7463..7729)
                     /gene="pmrD"
                     /locus_tag="J1Y83_002914"
     CDS             complement(7463..7729)
                     /gene="pmrD"
                     /locus_tag="J1Y83_002914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311174.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal transduction protein PmrD"
                     /protein_id="HBA7631045.1"
                     /translation="MEWLVKKSCCNKQDNRHVIMLCDAGGAIKMIAEVKSDFAVKVGD
                     LLSPLQNALYCINREKLHTVKVLSASCYSPDEWERQCKAAGKTQ"
     gene            complement(7839..9194)
                     /gene="menE"
                     /locus_tag="J1Y83_002915"
     CDS             complement(7839..9194)
                     /gene="menE"
                     /locus_tag="J1Y83_002915"
                     /EC_number="6.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate--CoA ligase"
                     /protein_id="HBA7631046.1"
                     /translation="MIFSDWPWRHWRQVRGEAIALRLNDEQLNWRELCARVDELASSF
                     AMQGVVEGSGVMLRAWNTPQTLLAWLALLQCGARVLPVNPQLPQPLLEELLPNLTLQF
                     ALVPEGENTFPALASLHIQLVKGAHAAAWQPTRLCSMTLTSGSTGLPKAAVHTYQAHL
                     ASAEGVLSLIPFGDHDDWLLSLPLFHVSGQGIMWRWLYAGARMTVRDKQPLEQMLAGC
                     THASLVPTQLWRLLVNRSSVSLKAVLLGGAAIPVELTEQAREQGIRCFCGYGLTEFAS
                     TVCAKEADGLADVGSPLPGREVKIVNDEVWLRAASMAEGYWRNGQRVPLVNDEGWYAT
                     RDRGEMYNGKLTIVGRLDNLFFSGGEGIQPEEVERVIAAHTAVLQVFIVPVADKEFGH
                     RPVAVVEYDQQSVDLDEWVKDKLARFQQPVRWLTLPPELKNGGIKISRQALKEWVQRQ
                     Q"
     gene            complement(9191..10153)
                     /gene="menC"
                     /locus_tag="J1Y83_002916"
     CDS             complement(9191..10153)
                     /gene="menC"
                     /locus_tag="J1Y83_002916"
                     /EC_number="4.2.1.113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate synthase"
                     /protein_id="HBA7631047.1"
                     /translation="MRSAQVYRWQIPMDAGVVLRDRRLKTRDGLYVCLREGEHEGWGE
                     ISPLPGFSQETWEDAQSVLLAWVNNWLAGDCEIPQMPSVAFGVSCALAELAETLPQAA
                     NYRAAPLCNGDPDDLILKLADMPGEKVAKVKVGLYEAVRDGMVVNLLLEAIPDLHLRL
                     DANRAWTPLKGQQFAKYVNPDYRHRIAFLEEPCKTRDDSRAFARETGIAIAWDESLRE
                     PDFAFVAEEGVRAVVIKPTLTGSLEKVREQVKAAHALGLTAVISSSIESSLGLTQLAR
                     IAAWLTPDTIPGLDTLDLMQAQQVRRWPGSLLPLVDVDAMERLL"
     gene            complement(10153..11010)
                     /gene="menB"
                     /locus_tag="J1Y83_002917"
     CDS             complement(10153..11010)
                     /gene="menB"
                     /locus_tag="J1Y83_002917"
                     /EC_number="4.1.3.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001641973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA synthase"
                     /protein_id="HBA7631048.1"
                     /translation="MIYPDEAMLYAPVEWHDCSEGFEDIRYEKSTDGIAKITINRPQV
                     RNAFRPLTVKEMIQALADARYDDNIGVIILTGAGDKAFCSGGDQKVRGDYGGYKDDSG
                     VHHLNVLDFQRQIRTCPKPVVAMVAGYSIGGGHVLHMMCDLTIAADNAIFGQTGPKVG
                     SFDGGWGASYMARIVGQKKAREIWFLCRQYDAKQALDMGLVNTVVPLADLEKETVRWC
                     REMLQNSPMALRCLKAALNADCDGQAGLQELAGNATMLFYMTEEGQEGRNAFNQKRQP
                     DFSKFKRNP"
     gene            complement(11025..11783)
                     /gene="menH"
                     /locus_tag="J1Y83_002918"
     CDS             complement(11025..11783)
                     /gene="menH"
                     /locus_tag="J1Y83_002918"
                     /EC_number="4.2.99.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-6-hydroxy-2,
                     4-cyclohexadiene-1-carboxylate synthase"
                     /protein_id="HBA7631049.1"
                     /translation="MILHAQAKHGKPGLPWLVFLHGFSGDCHEWQEVGEAFADYSRLY
                     VDLPGHGGSATISVDGFDDVTGLLCKTLVSYNILNFWLVGYSLGGRVVMMAACQELAG
                     LCGVVVEGGHPGLQNAEQRAERQRSDRQWAQRFRTEPLTAVFADWYQQPVFASLNDDQ
                     RRELVALRSNNNGATLAAMLEATSLAVQPDLRANLSARTFAFYYLCGERDSKFRALAA
                     ELAADCHVIPRAGHNAHRENPAGVIASLAQILRF"
     gene            complement(11780..13450)
                     /gene="menD"
                     /locus_tag="J1Y83_002919"
     CDS             complement(11780..13450)
                     /gene="menD"
                     /locus_tag="J1Y83_002919"
                     /EC_number="2.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-5-enolpyruvyl-6-hydroxy-3-
                     cyclohexene-1-carboxylic-acid synthase"
                     /protein_id="HBA7631050.1"
                     /translation="MSVSAFNRRWAAVILEALTRHGVRHICIAPGSRSTPLTLAAAEN
                     SAFIHHTHFDERGLGHLALGLAKVSKQPVAVIVTSGTAVANLYPALIEAGLTGEKLIL
                     LTADRPPELIDCGANQAIRQPGMFASHPTHSISLPRPTQDIPARWLVSTIDHALGTLH
                     AGGVHINCPFAEPLYGEMDDTGISWQQRLGDWWQDDKPWLREAPRRESEKQRDWFFWR
                     QKRGVVVAGRMSAEEGKKVALWAQTLGWPLIGDVLSQTGQPLPCADLWLGNAKATSEL
                     QQAQIVVQLGSSLTGKRLLQWQASCEPEEYWIVDDIEGRLDPAHHRGRRLIANIADWL
                     ELHPAEKRQPWCVEIPRLAEQAMQAVIARRDAFGEAQLAHRISDYLPEQGQLFVGNSL
                     VVRLIDALSQLPAGYPVYSNRGASGIDGLLSTAAGVQRASGKPTLAIVGDLSALYDLN
                     ALALLRQVSAPLVLIVVNNNGGQIFSLLPTPKSERERFYLMPQNVHFEHAAAMFELKY
                     HRPQNWQELETTLVDAWRTPTTTVIEMVVNDTDGAQTLQQLLAQVSHL"
     gene            complement(13539..14834)
                     /gene="menF"
                     /locus_tag="J1Y83_002920"
     CDS             complement(13539..14834)
                     /gene="menF"
                     /locus_tag="J1Y83_002920"
                     /EC_number="5.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311180.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate synthase MenF"
                     /protein_id="HBA7631051.1"
                     /translation="MQSLTTALENLLRHLSQEIPATPGIRVIDIPFPLKDAFDALSWL
                     ASQQVYPQFYWQQRNGDEEAAVLGAITRFTSLDQAQRFLRQHPEHADLRIWGLNAFDP
                     SQGNLLLPRLEWRRCGGKATLRLTLFSESSLQHDAIKAKEFIATLVSIKPLPGLHLTT
                     TREQHWPDKTGWTQLIELATKTIAEGELDKVVLARATDLHFASPVNAAAMMAASRRLN
                     LNCYHFYMAFDGENAFLGSSPERLWRRRDKALRTEALAGTVANHPDDKQAQQLGEWLM
                     ADDKNQRENMLVVEDICQRLQADTQTLDVLPPQVLRLRKVQHLRRCIWTSLNKADDVI
                     CLHQLQPTAAVAGLPRDLARQFIARHEPFTREWYAGSAGYLSLQQSEFCVSLRSAKIS
                     GNIVRLYAGAGIVRGSDPEQEWQEIDNKAAGLRTLLQME"
     gene            complement(14913..15218)
                     /gene="elaB"
                     /locus_tag="J1Y83_002921"
     CDS             complement(14913..15218)
                     /gene="elaB"
                     /locus_tag="J1Y83_002921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein ElaB"
                     /protein_id="HBA7631052.1"
                     /translation="MSNQFGDTRIDDDLTLLSETLEEVLRSSGDPADQKYVELKARAE
                     KALDDVKKRVSQASDSYYYRAKQAVYRADDYVHEKPWQGIGVGAAVGLVLGLLLARR"
     gene            complement(15273..15734)
                     /locus_tag="J1Y83_002922"
     CDS             complement(15273..15734)
                     /locus_tag="J1Y83_002922"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBA7631053.1"
                     /translation="MIDWQDLHHSDLSVSQLYALLQLRCAVFVVEQNCPYQDIDGDDL
                     EGENRHILGWHNGTLVAYARILKSDDELQPVVIGRVIVSEALRGEKIGQQLMSKALES
                     CTRRWPEKPIYLGAQAHLQNFYGQFGFIPVTDIYEEDGIPHIGMAREVIQA"
     gene            15799..16716
                     /gene="rbn"
                     /locus_tag="J1Y83_002923"
     CDS             15799..16716
                     /gene="rbn"
                     /locus_tag="J1Y83_002923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease BN"
                     /protein_id="HBA7631054.1"
                     /translation="MELIFLGTSAGVPTRTRNVTAILLNLQHPTQSGLWLFDCGEGTQ
                     HQLLHTAFNPGKLDKIFISHLHGDHLFGLPGLLCSRSMSGIIQPLTIYGPHGIREFVE
                     TALRISGSWTDYPLEIVEIGAGEIFDDGLRKVTAYPMEHPLECYGYRIEEHDKPGALN
                     AQALKAAGVPPGPLFQELKAGKTIMLDDGRQINGADYLAVPVPGKALAIFGDTGPCDA
                     ALELAKGVDVMVHEATLDMAMEAKANSRGHSSTRQAAALAREAGVGKLIITHVSSRYD
                     DKGCQHLLRECRSIFPATELANDFAVFSI"
     gene            complement(16837..18579)
                     /locus_tag="J1Y83_002924"
     CDS             complement(16837..18579)
                     /locus_tag="J1Y83_002924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001244797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HBA7631055.1"
                     /translation="MRNKNIIMLLISSLILSGCGPEPEDKKSQQQQSTTPTDQQVLAA
                     QQAATKVAEQSAAKAAADAKALAQQEVQQYSDKQALLGRLQAAPKYQHAAREKAASQI
                     ANPGTARYQQFDDNPVKQVAQNPLATFSLDVDTGSYANVRRFLNQGLLPPPDAVRVEE
                     IVNYFPSDWDIKDKQSIPASKPIPFAMRYELAPAPWNEQRTLLKVDILAKDRKSEELP
                     ASNLVFLIDTSGSMISDERLPLIQSSLKLLVKELREQDNIAIVTYAGDSRIALPSISG
                     SHKAEINAAIDSLDAEGSTNGGAGLEMAYQQAAKGFIKGGINRILLATDGDFNVGIDD
                     PKSIESMVKKQRESGVTLSTLGVGDSNYNEAMMVRIADVGNGNYSYIDTLSEAQKVLN
                     SEMRQTLITVAKDVKAQIEFNPAWVTEYRQIGYEKRQLRAEDFNNDNVDAGDIGAGKH
                     ITFLFELTLKGQKASIDKLRYAPDNKSAKSDKTKELAWLKIRWKSPQGKESQLVEFPL
                     AFAIKAPSEDMRFRAAVAAYGQKLRGSEYLNNTSWQQIKQWAQKAKGEDPQGYRAEFI
                     RLIGLAKDLDNSQN"
BASE COUNT         4311 a         4995 c         4699 g         4617 t
ORIGIN      
        1 tttcttaagg ttaagttaat attctatcct taaaattttg ctccaaatgg caaaatatac
       61 acaacactct ttatagcaaa tataagtgga caggtattca atgacggatg gaaaaacaat
      121 gtcaggattt ttgccttttt cgcgaccagc aatgggcgta gaggaactcg ctgcagttaa
      181 agaggttctc gaatccggtt ggatcacaac cggtccgaag aatcaggcgc ttgaacaagc
      241 tttttgccag ttgacgggaa atcagcatgc catcgcggtc agttcagcca ccgccggaat
      301 gcatatcacg ctaatggcgt tggaaattgg caagggcgat gaagtgatta cgccttccct
      361 gacctgggtt tcaaccctca atatgatttc cttgctgggt gcaacgccgg taatggtgga
      421 tgtcgactgc aatacgctga tggtcacgcc tgaagcgatc gaagcagcca ttacaccacg
      481 cactaaagcc atcattccgg tgcattatgc cggtgcgcca gcagatattg acgccattcg
      541 cgccattggc gaacgttacg gcatcgcagt tatcgaagat gctgcccatg ccgtcggtac
      601 gtattacaaa gggcgacata ttggcgcaaa aggtaccgct attttttcgt ttcacgccat
      661 caaaaatatt acctgtgcgg aaggtggcct gattgttact gataatgaaa atcttgcccg
      721 ccagctgcgg atgctgaaat ttcacggtct gggtgtcgat gcctatgaca gacatacctg
      781 gggccgtgca ccgcaggctg aagtcttaac gccgggctat aagtacaatc tgaccgatat
      841 taacgccgcg attgccctga cccagttagt caaattagag cacctcaaca cccggcggcg
      901 cgaaattgcc cagcaatatc agcaagcact ggcagctctc ccctttcagc cattaagcct
      961 tcccgcctgg ccgcacgttc acgcctggca tctgtttatt attcgtgtcg atgaacaacg
     1021 ttgtggtatc agtcgcgatg cgttgatgga agcgttaaaa gaaagaggca ttggtaccgg
     1081 gttacatttc cgcgccgctc acacacaaaa atattatcgc gagcgttttc ccacgctgtc
     1141 gttaccgaat accgaatgga atagcgaacg catctgttcg ttgccgctgt tcccggatat
     1201 gaccaccgcc gatgccgacc gcgtcatcac agcccttcag caactcgcag gacaatgagc
     1261 catgtttgaa atccaccctg ttaagaaagt ctcggtggtt attcccgttt ataacgagca
     1321 ggaaagctta ccggaattaa tcaggcgcac caccgcagcc tgtgaatcac tggggaaaga
     1381 gtatgaaatc ctgctgattg atgacggcag tagcgataat tccgcgcata tgctggtcga
     1441 agcctcacaa gcggagggca gccatattgt gtctatcttg cttaaccgca attacgggca
     1501 acattcagcg attatggcgg gattcagtca cgtcaccggc gacttaatta ttaccctcga
     1561 tgccgatctc cagaatccgc cagaggaaat cccccgtctg gtggcaaaag ccgatgaagg
     1621 ttacgacgtg gtagggactg tacgccagaa ccgccaggac agctggtttc gtaaaaccgc
     1681 ttcgaagatg attaaccggc ttattcagcg caccactggc aaagcgatgg gtgactatgg
     1741 ttgtatgctg cgcgcctatc gccgtcatat tgtcgatgcg atgttgcact gccatgaacg
     1801 cagcaccttt atcccgattc tggcgaatat cttcgcccgc cgtgccattg aaattccagt
     1861 gcaccatgcc gagcgtgagt ttggtgaatc caaatacagc tttatgcgcc tgattaattt
     1921 gatgtacgac ctggtgacct gccttaccac aacgccgcta cgtatgttga gtttgctcgg
     1981 cagcattatt gcgattggcg gttttagcat tgcggtgctg ctggtgattt tacgcctgac
     2041 cttcggacca caatgggcgg cagaaggcgt ctttatgcta tttgccgtgc tgtttacttt
     2101 tattggcgct cagtttatcg gcatgggatt gctcggtgaa tatatcggca ggatctacac
     2161 cgatgtccgc gcccgccccc gctattttgt tcagcaagtt atccgtccat ccagcaagga
     2221 aaatgaataa tgaaaaccgt cgtttttgcc taccacgata tgggatgcct cggtattgaa
     2281 gccctgctgt ctgccggtta cgaaattagc gccattttta cccatactga taaccccggt
     2341 gaaaaagcct tttatggttc ggttgcccga ctggcagcgg aaagaggcat tccagtttat
     2401 gcgccggata acgttaatca tccgctgtgg gtggaacgca ttgcccaact atcaccagag
     2461 gtgattttct ctttttatta tcgccatctt atttgcgatg agattttgca gctcgctccg
     2521 agaggtgcat ttaatctgca tggttcgctg ttaccaaaat atcgtggtcg cgcgccgctg
     2581 aactgggtgc tggtgaacgg tgaaacggaa actggcgtta cattgcaccg aatggtgaaa
     2641 cgtgccgatg ccggggccat tgtggcgcaa ctgcgcattg ccattgcgcc agacgatatc
     2701 gccattacgc tgcatcataa attgtgccat gccgcgcgcc agctactgga acagacatta
     2761 cccgccatta aacacggtaa tattctggaa atcgcccagc gcgaaaacga agccacctgt
     2821 tttggtcgca gaacgccgga tgacagtttc ctcgaatggc ataagccggc atccgtactg
     2881 cacaacatgg tgcgtgccgt tgccgatccg tggccgggtg ccttcagcta tgttggcaat
     2941 cagaaattca ccgtctggtc gtcgcgtgtt catcctcatg ccagcaaagc acagccgggg
     3001 agcgtgattt ctgttgcacc actgctgatt gcctgtggcg atggcgcgct ggaaatcgtc
     3061 actggacagg cgggcgacgg cattactatg cagggctcgc aattagcgca gacgctgggc
     3121 ctggtgcaag gttcacgctt gaatagccag cctgcctgtg ccgcccgacg ccgtacccgg
     3181 gtactcatcc tcggggtgaa tggctttatt ggcaaccatc tgacagaacg cctgctgcgc
     3241 gaagatcatt atgaagttta cggtctggat attggcagcg atgcgataag ccgttttctg
     3301 aatcatccgc attttcactt tgtcgaaggc gatatcagta ttcattccga atggattgag
     3361 tatcacgtca aaaaatgtga tgtcgtcttg cctttggtgg cgatagccac gccgattgaa
     3421 tatacccgca acccgctgcg cgtatttgaa ctcgatttcg aagagaatct gcgcattatc
     3481 cgctactgcg tgaagtaccg taagcgaatc atcttcccgt cgacttcaga agtttatggg
     3541 atgtgtagcg ataaatactt cgatgaggat cattctaatt taatcgtcgg cccggtgaat
     3601 aaaccacgct ggatttattc ggtgtctaaa caattacttg atcgagtgat ctgggcctat
     3661 ggcgaaaaag aaggtttaca gttcaccctc ttccgcccgt ttaactggat ggggccacga
     3721 ctggataacc ttaatgcagc gcgaattggc agctcccgcg ctattacgca actcattctc
     3781 aatctggtag aaggttcacc gattaagctg attgatggcg gaaaacaaaa acgctgcttt
     3841 actgatattc gggatggtat cgaggcgtta taccgcatta tcgaaaatgc gggaaatcgc
     3901 tgcgatggcg aaattatcaa cattggcaat cctgagaacg aagcgagcat tgaagaactg
     3961 ggtgagatgc tgctggcgag cttcgaaaaa catccgctgc gccattactt cccaccgttt
     4021 gcgggctttc gtgttgtcga aagtagcagc tactatggca aaggatatca ggacgtagag
     4081 catcgtaaac cgagcatccg caatgcccgc cgctgcctgg actgggaacc gaaaattgat
     4141 atgcaggaaa ccatcgacga aacgctggat ttcttcctgc gcaccgttga tcttacggat
     4201 aaaccatcat gaccaaagtc ggcttacgca ttgatgtcga tacctttcgt ggcacccgtg
     4261 aaggcgtgcc gcgtctgctg gaaatcttga gtaagcataa tattcaggcc agcatttttt
     4321 tcagcgtcgg cccggacaat atgggccgcc atctctggcg actggtgaag ccacagtttt
     4381 tgtggaagat gctgcgctca aacgcggcat cgctttatgg ctgggatatt ttactggcag
     4441 gtacggcctg gccaggtaaa gagattggtc atgccaatgc tgatatcatt cgtgaagcgg
     4501 ctaaacatca cgaagtcggc ctgcacgcct gggatcacca tgcctggcaa gcccatagcg
     4561 gtaattggga tcggcaaaca atgatcgacg atattgcacg cggccttcgc accctggaag
     4621 agattatcgg tcaaccggta acctgttctg ccgctgctgg ctggcgtgct gaccaacagg
     4681 tgatcgaagc aaaagaagcg ttccatttgc gctacaacag cgattgtcgt ggggcgatgc
     4741 cgtttcgtcc attgctcgaa tcaggaaccc ccggcactgc gcaaattccg gtgaccttac
     4801 ccacctggga tgaagtgatt ggtcgggatg tgaaagcaga agattttaac ggttggttac
     4861 tcaaccgcat ccagcgagat aaaggcacgc cggtttatac cattcatgcc gaagttgaag
     4921 gctgcgctta tcagcataat tttgtggatc tcctgaaacg cgcagctcag gaaggcgtga
     4981 cattttgccc tttaagcgaa ctgttatcag ggacgttgcc gctcggacaa gttgttcgcg
     5041 gaaatattgc cggacgtgaa ggctggctgg gttgccaaca aatggcgggt agtcactgat
     5101 gaaatcggta cgttacctta tcggcctctt cgcatttatt gcctgctatt acctgttacc
     5161 gatcagcacg cgtctgctct ggcaaccaga tgaaacgcgt tatgcggaaa tcagtcggga
     5221 aatgctggca tccggcgact ggattgttcc ccatctgtta gggctacgtt atttcgaaaa
     5281 acccattgcc ggatactgga ttaacagcat tgggcaatgg ctatttggcg cgaataactt
     5341 tggtgtgcgg gcaggcgtta tctttgcgac cctgttaact gccgcgctgg tgacctggtt
     5401 tactctgcgc ttatggcgcg ataaacgtct ggcattactc gccacagtaa tttatctctc
     5461 attgtttatt gtctatgcca tcggcactta tgccgtgctc gatccgttta ttgccttctg
     5521 gctggtggcg ggaatgtgca gcttctggct ggcaatgcag gcacagacgt ggaaaggcaa
     5581 aagcgcggga tttttactgc tgggaatcac ctgcggcatg ggggtgatga ccaaaggttt
     5641 tctcgccctt gccgtgccgg tattaagcgt gctgccatgg gtagcaacgc aaaaacgctg
     5701 gaaagatctc tttatttacg gctggctggc ggttatcagt tgcgtactga cggttctccc
     5761 ctggggactg gcgatagcgc agcgggagcc tgacttctgg cattattttt tctgggttga
     5821 gcatattcaa cgctttgcac tggatgatgc ccaacataga gcgccgttct ggtactactt
     5881 gccggtcgtc attgccggta gcctgccgtg gctaggatta ctccccggtg cactgtacgc
     5941 tggctggaaa aatcgcaagc attccgcaac cgtttatttg ttgagctgga cgataatgcc
     6001 gctgctgttt ttctccgtcg ctaaaggcaa attgcccacc tatattcttt cctgctttgc
     6061 acctctggca atactgatgg cgcattacgc gttgctggca gcaaaaaata atcctctggc
     6121 gttgcggatt aatggctgga ttaatatcgc ttttggcgtc actggcatta ttgccacatt
     6181 tgtggtctcc ccctggggac caatgaacac accagtgtgg caaaccttcg agagctataa
     6241 agtcttttgt gcctggtcta ttttttcgct atgggcattt ttcggctggt acaccttaac
     6301 aaacgtcgaa aagacctggc cttttgccgc gctttgcccg ctggggctgg cgttgctggt
     6361 aggattttca attcctgaca gagtcatgga aggaaaacat ccgcaatttt ttgtcgagat
     6421 gacacaagaa tcacttcagc caagccgcta tattcttact gatagcgtcg gtgttgccgc
     6481 aggtctggca tggagcctgc aacgcgatga catcatcatg tatcgccaga caggtgagtt
     6541 gaaatatggc cttaattatc cggatgcaaa agggagattt gtcagcggtg atgagttcgc
     6601 aaactggctt aatcaacatc gtcaggaggg gattattaca ctcgtgcttt cggttgaccg
     6661 cgatgaagat atcaatagtc tcgccattcc gcccgcagat gtcatcgatc gtcaggagcg
     6721 tctggtgctg attcagtatc ggcccaaatg atctggctaa cattagtctt tgccagcttg
     6781 cttagcgttg ccgggcagtt gtgtcagaaa caggcaacct gctttgcggc ggtaaacaaa
     6841 cggcgcaaac atatcgtgct gtggctggga ctggcgctgg cttgtcttgg ccttgcaatg
     6901 gtgctctggc tgctggtctt gcagaacgta ccggtaggca ttgcctaccc gatgttaagt
     6961 ctgaattttg tctgggtaac gctggctgca gtaaaactgt ggcacgagcc ggtatcgctg
     7021 cgtcactggt gtggggtggc gttcattatt ggcggcattg tgatcctcgg gagtacggtg
     7081 taatgggcct gatgtgggga ttattcagcg tcattatcgc ctccgctgcg caattaagcc
     7141 tgggttttgc ggcgagtcat ctgccgccaa tgacgcactt atgggatttt atcgcggcgc
     7201 tgttagcttt tggcctggat gcccggatac tgttactcgg gctgctgggt tatctgcttt
     7261 ccgttttttg ttggtataaa acactacata aacttgcact aagcaaagcc tatgccttgc
     7321 tcagtatgag ttatgtgctg gtatggattg cttcaatggt acttcccggc tgggaaggga
     7381 ctttttcgct gaaagcacta ctgggagtag cctgtattat gagcgggttg atgctgattt
     7441 tcctgcccac gacaaaacaa cgttactgag ttttccctgc cgctttgcac tgccgttccc
     7501 actcatcggg gctataacag cttgcagaaa ggactttcac cgtgtgcagc ttttcacgat
     7561 taatacagta aagcgcattc tgcaaaggcg agagtaaatc ccccactttc acggcgaagt
     7621 cactcttcac ttcggcgatc atttttatcg cgccgccagc atcgcagagc ataattacat
     7681 gtctgttatc ttgtttattg cagcacgatt ttttgaccag ccattccatt gcattatcct
     7741 gtttgctaag agttttcaca tcaatttaca gattaacaga taaactcttt atcaacggca
     7801 atggaatggc agaaatgaga agtgaaacgg agggttaatt attgctggcg ttgcacccac
     7861 tcttttagcg cctgacgtga aattttaata ccgccgtttt tcagctccgg cggcagagtc
     7921 agccagcgca ccggttgttg aaaacgagcc agcttatctt tcacccattc gtcaagatca
     7981 acgctttgct ggtcatactc caccaccgct accggccgat gaccaaactc cttgtcggca
     8041 acgggaacga taaacacctg caaaaccgca gtatgtgcag caataacgcg ctcgacttct
     8101 tccggctgaa taccctcacc gccactgaag aatagattgt ctaaacgtcc gacaatggtc
     8161 aacttgccat tatacatctc accgcgatcg cgcgtggcgt accagccttc gtcattaacc
     8221 agtggaaccc gttgcccgtt acgccagtaa ccttctgcca tactggcagc ccgcagccac
     8281 acttcatcat taacgatttt cacttcccga cccggcagcg gcgaaccaac gtctgccaga
     8341 ccgtcggctt ctttcgcaca cactgtggag gcaaactcgg tcagaccata gccgcaaaag
     8401 caccgaatcc cctgctcgcg cgcctgttcc gtcaactcga ccgggatagc cgcgccgcca
     8461 agtaaaaccg ctttcaggga gacggaacta cggttaacca gcaaacgcca gagttgtgtt
     8521 ggcaccagtg aagcgtgagt acagcctgcc agcatctgct ccaatggctg tttatcacgc
     8581 accgtcatcc gcgcaccagc gtataaccag cgccacataa ttccctgacc ggagacgtga
     8641 aacagcggta aagagagcaa ccagtcatcg tgatcgccaa acggaatcag cgataacacc
     8701 ccttcggcgc tggcaagatg agcctgataa gtatgaacag cggctttcgg caagccagtg
     8761 gaaccagagg ttaacgtcat tgagcacaga cgcgtcggct gccacgccgc ggcatgtgcg
     8821 cctttaacca gctgaatgtg cagcgacgct aacgccggaa acgtgttttc cccttccggc
     8881 accagcgcaa attgcagcgt aagattgggc agcaattcct caagcagcgg ttgcggcagc
     8941 tgagggttca cgggcaacac ccgcgccccg cattgcagta acgccagcca ggccagcagc
     9001 gtttgcggcg tattccacgc ccgcaacatc acgccgctgc cctcaaccac accctgcatc
     9061 gcaaagctgg aggctaattc atcgacgcga gcacaaagct cgcgccagtt gagttgctcg
     9121 tcattaagac gtaaggcgat ggcttctccc cgcacttgcc gccagtgacg ccacggccag
     9181 tcagagaaga tcataacagc cgctccattg catcaacgtc cactaacggc agcaggctcc
     9241 ccggccagcg acgaacctgc tgcgcctgca tcagatccag cgtgtccagt cccggaatgg
     9301 tgtctggcgt taaccaggca gcaatccgcg ccagttgcgt tagacctaag ctcgactcaa
     9361 tggaagagct gatcaccgcc gtcagcccca gcgcatgcgc cgccttcacc tgctcgcgta
     9421 ctttctccag gctgcccgtg agcgtgggtt tgataactac cgcgcgcacg ccctcttcag
     9481 ctacaaaggc aaaatccggc tcgcgcaagc tttcatccca ggcaatggca atgccggttt
     9541 cacgggcaaa cgcacgcgaa tcatcgcggg ttttgcacgg ctcttcgaga aacgcgatgc
     9601 ggtggcgata atccgggtta acgtatttgg caaactgctg gcctttcagc ggtgtccagg
     9661 cgcggttcgc gtcaagacgc aaatgcagat ccggaattgc ctccagcaac agattcacca
     9721 ccatgccgtc gcgcacggct tcgtacaatc ccactttgac cttcgccact ttctcgccag
     9781 gcatatcggc aagtttgagg atcagatcgt caggatcgcc attacacagc ggcgccgcac
     9841 ggtaattggc cgcctgcggg agcgtctctg ccagctccgc taatgcacaa cttacgccaa
     9901 aggcaacgga aggcatctgc ggtatctcgc aatcgcctgc cagccagtta tttacccagg
     9961 caagcagcac actttgcgca tcttcccagg tttcctgact gaagcccggt agtggggaga
    10021 tctcccccca cccttcgtgc tcgccctcac gcaggcaaac atacagcccg tcgcgggttt
    10081 ttaaccgcct gtcgcgcaga accacccccg cgtccatggg gatctgccag cggtatacct
    10141 gcgcactacg cattacggat tccgtttgaa tttgctgaaa tcaggctggc gtttctggtt
    10201 gaaagcattg cgcccttcct gaccttcttc ggtcatgtag aacagcatgg tggcattacc
    10261 cgccagttcc tgcagaccgg cctgaccgtc acagtcggcg ttcagcgcag ctttcaggca
    10321 gcgcagcgcc atcgggctgt tttgcagcat ttcacggcac caacggacgg tttctttttc
    10381 cagatccgcc agcggtacaa cggtgttcac cagacccata tccagcgcct gttttgcgtc
    10441 gtactgacgg cacaggaacc agatttcacg tgcttttttc tgcccaacaa tgcgcgccat
    10501 ataggaagcg ccccagccgc cgtcgaagga accgactttc gggccagttt ggccgaagat
    10561 ggcattatca gccgcgatag tcaggtcgca catcatgtgc agaacgtgac cgccgccgat
    10621 ggagtagcca gccaccatcg cgacaaccgg tttcggacag gtacggatct gacgctggaa
    10681 gtccagcacg ttcaggtgat gtacgccgga atcatcttta tagccgccgt aatcaccacg
    10741 tactttctgg tcaccaccgg agcagaacgc tttatcgcct gcaccagtca gaatgatcac
    10801 gccgatgttg tcgtcataac gcgcatccgc cagcgcctgg atcatctctt ttaccgtcag
    10861 aggacggaag gcattgcgca cctgcggacg attaatggtg atttttgcga taccgtcggt
    10921 ggatttttca taacgaatgt cctcaaaacc ttcggagcag tcgtgccatt caaccggtgc
    10981 gtaaagcatt gcttcatcag gataaatcat attgtgtcct ttagtcagaa acgcaagatc
    11041 tgcgccagac ttgcgattac gccagcggga ttttcccggt gcgcgttatg tccggcgcga
    11101 ggaatgacat ggcagtcggc agccagttcc gccgccagag cgcggaattt gctgtcacgt
    11161 tcaccacata aataataaaa cgcaaatgtg cgggcgctaa ggttagcacg taaatcgggc
    11221 tggacggcga gagaagtcgc ctccagcatg gcggcaaggg ttgcgccatt attgttgctg
    11281 cgcagcgcca ccagctcccg gcgttgatcg tcattgagcg aggcaaaaac aggctgttga
    11341 taccaatcgg caaataccgc cgttaacggt tctgtacgaa aacgctgcgc ccattggcga
    11401 tcggaacgct gacgttccgc gcgttgttca gcattttgca acccaggatg cccgccttcg
    11461 acaacaactc cacaaagccc cgccagctcc tggcaagccg ccatcatcac cacccgtccg
    11521 ccaagcgagt accccaccag ccagaagtta aggatgttgt aactaaccag cgttttacac
    11581 aataagccgg tgacatcatc aaatccatcg acgctgatcg tcgctgaacc accgtgacct
    11641 gggagatcaa catacaaccg cgagtagtcg gcaaacgcct cgcccacttc ttgccattca
    11701 tggcaatcgc cagaaaaacc gtgcaaaaac accaaccagg gtaaacctgg tttgccgtgt
    11761 tttgcctgcg cgtgcaggat cataaatggc ttacctgcgc cagaagctgc tggagcgttt
    11821 gcgcaccatc ggtgtcgtta accaccattt caatcaccgt ggtggtcggc gtacgccagg
    11881 catcgactag tgtcgtttca agttcctgcc agttttgcgg acgatgatat ttcagctcga
    11941 acatcgcggc ggcgtgctca aaatggacgt tttgcggcat cagatagaaa cgctcgcgct
    12001 cgcttttcgg cgttggcaac agcgaaaaaa tttgcccgcc gttgttgttc accacaatta
    12061 ataccagcgg tgcggaaacc tggcgcaata acgccagcgc gttgagatcg taaagtgcgg
    12121 agagatcgcc cacaatcgcc agcgtcggtt tgccgcttgc ccgctgaacg ccggcggcag
    12181 tcgagagcag cccgtcgata ccgctggcac cacggttgct gtacaccggg taacctgccg
    12241 gaagttgcga aagcgcatca atcagacgca ccaccaggct gttaccgaca aacaattgcc
    12301 cctgttcagg caagtagtcg ctgatgcgat gcgccagttg cgcttcgcca aacgcatcgc
    12361 gacgggcaat aaccgcctgc attgcctgtt ccgccaggcg cgggatttca acgcaccagg
    12421 gctggcgttt ttctgccgga tgcagctcca gccagtcggc aatattggca attaagcgac
    12481 gtccgcgatg gtgtgccgga tcaagtcgcc cttcaatgtc atcaacaatc cagtactctt
    12541 ctggttcaca gcttgcctgc cattggagga gtcgtttccc cgtcaggctg cttcccagtt
    12601 gcaccactat ttgtgcctgt tgcagttcgc tggtcgcttt ggcattgcct aaccagagat
    12661 cggcacacgg cagcggctgt ccggtttgcg acagcacgtc gccaatcagc ggccagccaa
    12721 gagtttgcgc ccacagtgca acttttttgc cctcttccgc actcatgcgc ccggcaacca
    12781 ccacgccgcg cttttgtcgc cagaagaacc agtcgcgctg tttttcactt tcccgacgag
    12841 gcgcttcacg cagccacggt ttgtcgtcct gccaccagtc gcccagccgc tgttgccagc
    12901 taatcccggt gtcgtccatt tcgccataca gcggttcagc aaacgggcag ttgatatgga
    12961 ctccaccagc atgaagcgta ccgagagcgt ggtcgatggt agaaaccagc caacgtgcgg
    13021 ggatatcctg ggtcgggcgc ggcagtgaaa tactgtgcgt ggggtgagag gcgaacattc
    13081 ccggctgacg aatcgcctga ttcgcaccgc agtcaatcag ctccggcggg cgatcggcgg
    13141 ttaacaggat cagtttttct ccggttaacc cagcttcaat cagtgccgga tagagatttg
    13201 ctaccgccgt gccggaggtc acaatcaccg ccacaggctg cttgctgact ttcgccagcc
    13261 ccagtgccag atgccccagt ccacgctcat cgaaatgggt atgatgaatg aatgcggaat
    13321 tctccgccgc cgctaacgtt aacggtgtag aacgcgagcc aggggcgata cagatgtgtc
    13381 tgacgccgtg acgcgttaat gcttccagaa tgaccgccgc ccagcgtcgg ttaaatgcgc
    13441 ttactgacat gagtttgtcc ggtatcaata ttgcggctaa gtataaggag ctacaaaaat
    13501 agattattga tatgaatcgg taatgatgcg actcattact attccatttg taataaagta
    13561 cgcagccctg ccgctttgtt gtcgatttcc tgccactctt gctcggggtc agaaccacgg
    13621 acaatgcctg cgccagcata tagccgcacg atattgccgc tgatttttgc tgagcgcagg
    13681 gaaacgcaga attcactttg ctgtaatgag agatagcccg cagaaccggc gtaccattct
    13741 cgagtgaacg gttcgtgacg ggcgataaac tgtcgcgcca gatcgcgcgg taagccagca
    13801 actgctgccg ttggctgcaa ctgatgtaaa cagatcacat catccgcttt gttgagtgaa
    13861 gtccagatac agcggcgaag atgctgtact ttacgtagac gcagtacctg cggcggtaaa
    13921 acatccagcg tttgggtatc ggcctgcaat cgttggcaga tatcttccac caccaacatg
    13981 ttctcgcgct ggtttttatc atccgccatc agccactcac ctaactgctg cgcctgctta
    14041 tcatcaggat gatttgctac cgttcccgcc agcgcttcgg tacgcagcgc tttgtcacgc
    14101 cgccgccata accgttccgg tgaagaacca agaaaagcat tttcgccatc aaaggccatg
    14161 taaaaatggt agcaattcag attgagccga cgactggcag ccatcatcgc tgccgcattg
    14221 accggacttg cgaaatgcag gtcagttgcc cgagcaagca caactttgtc gagctcgcct
    14281 tcggcgatgg ttttcgttgc cagttcgatt aattgcgtcc agcccgtttt gtccggccag
    14341 tgttgttctc gcgtggtggt taaatgtaat ccaggcaagg gcttgatact caccagtgtg
    14401 gcgataaatt cttttgcctt gattgcatcg tgctgaaggg agctttcgct gaatagcgtc
    14461 aaccgcagcg tggctttacc gccacaacgt cgccattcca ggcgtggtaa aagtaaattg
    14521 ccctgcgacg ggtcaaacgc attcaacccc cagatgcgta agtcggcgtg ttccgggtgc
    14581 tggcgaagaa aacgttgtgc ctggtccaac gacgtaaaac gggtaatcgc ccccaggacg
    14641 gcagcttctt catcaccatt acgttgttgc cagtagaatt gcggatacac ctgctgactg
    14701 gccagccagc tcaaggcatc aaaagcgtct ttgagaggga aaggaatatc gataacccga
    14761 atgccgggtg tcgccggaat ctcttgcgac aaatggcgca gtagattttc cagcgccgta
    14821 gtaagtgatt gcacgcggac ctctccctgt taaaagcctc acattatacg gggtacgaca
    14881 aaaaaaatgc agtaccccgg tgtagggagg ttttaacggc gtgccagcaa cagtcctaga
    14941 accagcccaa cggccgcacc cacaccaatt ccttgccagg gtttttcgtg gacgtagtca
    15001 tcagcacgat aaacagcctg cttcgcccga tagtaataac tgtctgaagc ctggctaacc
    15061 cgttttttca catcatccag cgctttttct gcacgcgctt tcagctcaac atatttctga
    15121 tcggcgggat cgccagagga gcggagcacc tcttccagtg tttcactaag cagcgtcagg
    15181 tcgtcatcaa tacgtgtatc accaaactga ttagacattc tcgttctcca tttgtgtaaa
    15241 acctgtcact aactatagac aagggttatt gattacgcct gaattacctc gcgtgccatc
    15301 ccaatgtgtg ggataccatc ttcttcataa atatcggtca cagggatgaa accaaattgc
    15361 ccataaaagt tttgtaaatg cgcctgcgcc cccagataaa tcggcttctc cggccagcgg
    15421 cgagtacacg attccagcgc cttactcatc aattgctgac cgattttttc accgcgtaag
    15481 gcttcgctga caatcacccg accaatcaca acgggttgca gttcgtcgtc acttttcaga
    15541 atcctcgcat acgccaccag tgtcccgtta tgccaaccga gaatgtggcg attttctccc
    15601 tccagatcgt caccatcgat atcctggtaa ggacagtttt gttccactac aaataccgca
    15661 caacgcagtt gtaataaggc ataaagctga gaaacggata aatcagaatg atgcagatct
    15721 tgccagtcga tcatggaagg ctcctttgtg ctgtttgctt tatactagcc ggaatatttt
    15781 cagcagcgtg atgaactcat ggaattaatt tttttaggta cttcagccgg tgtgccaacc
    15841 cgtacgcgta atgtcacggc aatattgctt aacctgcaac atccgaccca gagcggactt
    15901 tggttatttg actgcggtga aggcacccag catcagctac tgcataccgc ctttaaccct
    15961 ggaaaactgg acaagatttt tatcagtcac cttcatggcg atcatctctt tggtttaccc
    16021 ggcttactgt gcagtcgttc tatgtcaggc attatccaac ccttaacgat ttatggtccg
    16081 cacggtatcc gtgaatttgt ggagaccgcg ctgcggatta gcggctcatg gaccgattat
    16141 ccgctggaaa ttgtcgaaat tggcgctggc gaaattttcg atgatggcct gcgcaaagtg
    16201 accgcttatc cgatggaaca cccactggaa tgttatggtt atcgtattga agaacatgat
    16261 aaaccaggtg cattaaatgc ccaggcatta aaagctgctg gcgtgccgcc tggcccgctg
    16321 tttcaggaat taaaagcggg caaaacaatc atgctggatg atggaaggca gattaatggc
    16381 gcagattact tagccgttcc agtgccgggt aaagcgctcg ctattttcgg cgataccggc
    16441 ccctgcgatg ccgcgctgga gctggctaaa ggcgtcgacg taatggttca cgaagcgact
    16501 ctggatatgg cgatggaggc caaagccaac agtcgcggcc atagttctac acgccaggcg
    16561 gcggcactag cccgtgaggc tggagtcggt aagctaatca ttacccacgt cagctcgcgc
    16621 tatgatgaca aaggttgtca gcacctgtta cgtgaatgtc ggtcaatttt cccggcgact
    16681 gaactggcga atgatttcgc ggtgtttagc atttaagtcg aaagcggtgg ttagttagca
    16741 gattaattta acgataccaa cgcatttatt tgacattttt tcatctgttt gccggatacg
    16801 tttgatgtcg atatcaggca aacagaatta tcatttttag ttttgtgaat tgtccagatc
    16861 tttcgccagt ccaatcaggc gaataaattc cgctctgtaa ccctgtggat cttccccttt
    16921 tgccttctga gcccactgtt tgatctgctg ccaggaggta ttattcaggt attcagaacc
    16981 gcgtaacttt tgcccatatg cagccactgc tgcgcgaaaa cgcatatctt ctgaaggtgc
    17041 ctttattgcg aatgccaacg ggaattcaac taactgactt tcttttccct gtggggattt
    17101 ccaacgaatc tttaaccagg caagttcttt tgttttgtcc gatttcgctg atttgttatc
    17161 cggggcatag cgtaacttat caattgatgc tttttgccct ttcagcgtta attcaaataa
    17221 aaatgttata tgtttacctg cgccaatatc acccgcatca acgttgtcgt tattaaaatc
    17281 ctctgcccta agttggcgct tttcataacc tatctgacgg tattccgtta cccacgcggg
    17341 gttaaactca atttgcgctt tgacatcttt tgctacggta atcaacgtct gccgcatttc
    17401 actgttcaac actttctgcg cctctgagag ggtatcaatg tagctatagt tgccgttacc
    17461 aacatcggca attcttacca tcattgcctc gttataattg ctatccccca cgccaagcgt
    17521 cgacagagta acaccggact cacgctgttt tttgaccatt gattcaatcg attttggatc
    17581 gtcaatgcca acgttaaagt caccgtcagt ggccaataaa atgcgattga taccaccttt
    17641 aataaacccc ttcgctgcct gctgataagc catctccagc ccggcaccgc cattggtact
    17701 gccttctgca tccagcgaat caattgcagc attaatttcc gccttgtgac taccggagat
    17761 agaaggcaac gcaatacgag agtcgccagc gtaggtcacg atggcaatgt tatcctgctc
    17821 acgaagttct ttgaccaata atttcaacga agactggata agtggcaaac gttcatcaga
    17881 aatcattgaa ccagacgtgt cgataagaaa gaccagatta gaagcgggta actcttcact
    17941 tttgcgatct ttcgccagga tatcaacttt cagcaatgtt cgctgttcat tccagggtgc
    18001 aggtgccaat tcgtagcgca tagcgaaagg tattggctta gaggccggaa tagattgttt
    18061 gtctttgata tcccagtcag acgggaaata attgactatc tcctccaccc gcacagcgtc
    18121 tggcggaggt aacagccctt gattaaggaa acgccgcaca ttcgcataac tgcctgtatc
    18181 gacgtcaagg ctaaacgtcg ccaacgggtt ttgcgctacc tgcttaaccg gattatcatc
    18241 gaactgctgg tagcgagcgg ttcctggatt tgcgatttgg gaggctgctt tttctctggc
    18301 tgcatgttga tattttggag ccgcctgcaa tcgccccagc aaagcctgtt tgtctgaata
    18361 ttgttgcact tcttgctggg ctaaggcttt cgcgtctgca gcggctttgg cggcgctttg
    18421 ctcagccact tttgttgccg cttgttgcgc ggcaagcact tgctggtcag tgggtgtagt
    18481 agattgttgt tgctgacttt tcttatcctc aggttccggc ccacatcctg acaaaatcaa
    18541 actacttata agcaacatga ttatattttt atttcgcata taaaatcctt ttaaataaat
    18601 tcagggcccc gacataaaat ac
//
LOCUS       DADSYM010000099        18577 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2415
            SAMN08039579-rid11723173.denovo.099, whole genome shotgun sequence.
ACCESSION   DADSYM010000099 DADSYM010000000
VERSION     DADSYM010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039579
            Sequence Read Archive: SRR6362533
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18577)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18577)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980162.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 03:39:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,484
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 4,347
            CDSs (with protein)               :: 4,347
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 43 of 85
            Pseudo Genes (incomplete)         :: 37 of 85
            Pseudo Genes (internal stop)      :: 19 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18577
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039579-rid11723173.denovo.099"
                     /strain="LGE2415"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8302 N 95.9628 W"
                     /collection_date="2015-08-24"
     gene            <1..895
                     /locus_tag="J1Y83_002925"
     CDS             <1..895
                     /locus_tag="J1Y83_002925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HBA7631056.1"
                     /translation="NALPPTPRHASPEQLEKTVRYLTQTVHPRSADNIDNLNRSAEYI
                     KEVFISSGARVTAQDVPITGGPYKNIVANYGPADGPLIIIGAHYDSVSSYENDQLTYT
                     PGADDNASGVAGLLELARLLQQQTPGIGVQLVAYASEEPPFFRSDEMGSAVHAASLER
                     PVKLMIALEMIGYYDSTPGSQDYPSPAMSWLYPDRGDFIAVVGRMQDINAVRQVKAAL
                     LSSRDLSVYSMNAPGFIPGIDFSDHLNYWQHDIPAVMITDTAFYRNKQYHLPGDIADR
                     LNYQKMAQVVDGVTTLLYNSK"
     gene            998..1501
                     /locus_tag="J1Y83_002926"
     CDS             998..1501
                     /locus_tag="J1Y83_002926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbM family protein"
                     /protein_id="HBA7631057.1"
                     /translation="MGMIGYFAEIDSEKINQLLESTKKPLMDNIHDTLSGLRRLDIDK
                     RWDFLHFGLTGTSAFDPAKNDPLSRAVLGEHSLEDGIDGFLGLTWNQELAATIDRLES
                     LDRSELRKQFSIKRLNEMEIYPGVTFSEELEGQLFASIMLDMEKLISAYRRMLRQGNH
                     ALTVIVG"
     gene            complement(1568..3025)
                     /gene="nuoN"
                     /locus_tag="J1Y83_002927"
     CDS             complement(1568..3025)
                     /gene="nuoN"
                     /locus_tag="J1Y83_002927"
                     /EC_number="1.6.5.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416779.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoN"
                     /protein_id="HBA7631058.1"
                     /translation="MTITPQNLIALLPLLIVGLTVVVVMLSIAWRRNHFLNATLSVIG
                     LNAALVSLWFVGQAGAMDVTPLMRVDGFAMLYTGLVLLASLATCTFAYPWLEGYNDNK
                     DEFYLLVLIAALGGILLANANHLASLFLGIELISLPLFGLVGYAFRQKRSLEASIKYT
                     ILSAAASSFLLFGMALVYAQSGDLSFVALGKNLGDGMLNEPLLLAGFGLMIVGLGFKL
                     SLVPFHLWTPDVYQGAPAPVSTFLATASKIAIFGVVMRLFLYAPVGDSEAIRVVLAII
                     AFASIIFGNLMALSQTNIKRLLGYSSISHLGYLLVALIALQTGEMSMEAVGVYLVGYL
                     FSSLGAFGVVSLMSSPYRGPDADSLFSYRGLFWHRPILAAVMTVMMLSLAGIPMTLGF
                     IGKFYVLAVGVQAHLWWLVGAVVVGSAIGLYYYLRVAVSLYLHAPEQPGRDAPSNWQY
                     SAGGIVVLISALLVLVLGVWPQPLISIVRLAMPLM"
     gene            complement(3032..4561)
                     /gene="nuoM"
                     /locus_tag="J1Y83_002928"
     CDS             complement(3032..4561)
                     /gene="nuoM"
                     /locus_tag="J1Y83_002928"
                     /EC_number="1.6.5.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HBA7631059.1"
                     /translation="MLLPWLILIPFIGGFLCWQTERFGVKVPRWIALVTMGLTLALSL
                     QLWLQGGYSLTQSAGIPQWQSEFDMPWIPRFGISIHLAIDGLSLLMVVLTGLLGVLAV
                     LCSWKEIEKYQGFFHLNLMWILGGVIGVFLAIDMFLFFFFWEMMLVPMYFLIALWGHK
                     ASDGKTRITAATKFFIYTQASGLVMLIAILALVFVHYNATGVWTFNYEELLNTPMSNG
                     VEYLLMLGFFIAFAVKMPVVPLHGWLPDAHSQAPTAGSVDLAGILLKTAAYGLLRFSL
                     PLFPNASAEFAPIAMWLGVIGIFYGAWMAFAQTDIKRLIAYTSVSHMGFVLIAIYTGS
                     QLAYQGAVIQMIAHGLSAAGLFILCGQLYERIHTRDMRMMGGLWSKMKWLPALSLFFA
                     VATLGMPGTGNFVGEFMILFGSFQVVPVITVISTFGLVFASVYSLAMLHRAYFGKAKS
                     QIASQELPGMSLRELFMILLLVVLLVLLGFYPQPILDTSHSAIGNIQQWFVNSVTTTR
                     P"
     gene            complement(4725..6566)
                     /gene="nuoL"
                     /locus_tag="J1Y83_002929"
     CDS             complement(4725..6566)
                     /gene="nuoL"
                     /locus_tag="J1Y83_002929"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HBA7631060.1"
                     /translation="MNMLALTIILPLIGFVLLAFSRGRWSENVSAIVGVGSVGLAALV
                     TAFIGVDFFANGEQAYSQPLWTWMSVGDFNIGFNLVLDGLSLTMLSVVTGVGFLIHMY
                     ASWYMRGEEGYSRFFAYTNLFIASMVVLVLADNLLLMYLGWEGVGLCSYLLIGFYYTD
                     PKNGAAAMKAFVVTRVGDVFLAFALFILYNELGTLNFREMVELAPAHFADGNNMLMWA
                     TLMLLGGAVGKSAQLPLQTWLADAMAGPTPVSALIHAATMVTAGVYLIARTHGLFLMT
                     PEVLHLVGIVGAVTLLLAGFAALVQTDIKRVLAYSTMSQIGYMFLALGVQAWDAAIFH
                     LMTHAFFKALLFLASGSVILACHHEQNIFKMGGLRKSIPLVYLCFLVGGAALSALPLV
                     TAGFFSKDEILAGAMANGHINLMVAGLVGAFMTSLYTFRMIFIVFHGKEQIHAHAVKG
                     VTHSLPLIVLLILSTFVGALIVPPLQGVLPQTTELAHGSMLTLEITSGVVAVVGILLA
                     AWLWLGKRTLVTSIANSAPGRLLGTWWYNAWGFDWLYDKVFVKPFLGIAWLLKRDPLN
                     SMMNIPAVLSRFAGKGLLLSENGYLRWYVASMSIGAVVVLALLMVLR"
     gene            complement(6563..6865)
                     /gene="nuoK"
                     /locus_tag="J1Y83_002930"
     CDS             complement(6563..6865)
                     /gene="nuoK"
                     /locus_tag="J1Y83_002930"
                     /EC_number="1.6.5.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015833671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HBA7631061.1"
                     /translation="MIPLQHGLILAAILFVLGLTGLVIRRNLLFMLIGLEIMINASAL
                     AFVVAGSYWGQTDGQVMYILAISLAAAEASIGLALLLQLHRRRQNLNIDSVSEMRG"
     gene            complement(6862..7416)
                     /gene="nuoJ"
                     /locus_tag="J1Y83_002931"
     CDS             complement(6862..7416)
                     /gene="nuoJ"
                     /locus_tag="J1Y83_002931"
                     /EC_number="1.6.5.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HBA7631062.1"
                     /translation="MEFAFYICGLIAILATLRVITHTNPVHALLYLIISLLAISGVFF
                     SLGAYFAGALEIIVYAGAIMVLFVFVVMMLNLGGSEIEQERQWLKPQVWIGPAILSAI
                     MLVVIVYAILGVNDQGIDGTPISAKAVGITLFGPYVLAVELASMLLLAGLVVAFHVGR
                     EERAGEVLSNRKDDSAKRKTEEHA"
     gene            complement(7428..7970)
                     /gene="nuoI"
                     /locus_tag="J1Y83_002932"
     CDS             complement(7428..7970)
                     /gene="nuoI"
                     /locus_tag="J1Y83_002932"
                     /EC_number="1.6.5.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015959823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoI"
                     /protein_id="HBA7631063.1"
                     /translation="MTLKELLVGFGTQVRSIWMIGLHAFAKRETRMYPEEPVYLPPRY
                     RGRIVLTRDPDGEERCVACNLCAVACPVGCISLQKAETKDGRWYPEFFRINFSRCIFC
                     GLCEEACPTTAIQLTPDFEMGEYKRQDLVYEKEDLLISGPGKYPEYNFYRMAGMAIDG
                     KDKGEAENEAKPIDVKSLLP"
     gene            complement(7985..8962)
                     /gene="nuoH"
                     /locus_tag="J1Y83_002933"
     CDS             complement(7985..8962)
                     /gene="nuoH"
                     /locus_tag="J1Y83_002933"
                     /EC_number="1.6.5.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015875065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HBA7631064.1"
                     /translation="MSWISPELIEILLTVLKAVVILLVVVTCGAFMSFGERRLLGLFQ
                     NRYGPNRVGWGGSLQLVADMIKMFFKEDWIPKFSDRVIFTLAPMIAFTSLLLAFAIVP
                     VSPGWVVADLNIGILFFLMMAGLAVYAVLFAGWSSNNKYSLLGAMRASAQTLSYEVFL
                     GLSLMGVVAQAGSFNMTDIVNSQAHVWNVIPQFFGFITFAIAGVAVCHRHPFDQPEAE
                     QELADGYHIEYSGMKFGLFFVGEYIGIVTISALMVTLFFGGWQGPLLPPFIWFALKTA
                     FFMMMFILIRASLPRPRYDQVMSFGWKICLPLTLINLLVTAAVILWQAQ"
     gene            complement(8959..11685)
                     /gene="nuoG"
                     /locus_tag="J1Y83_002934"
     CDS             complement(8959..11685)
                     /gene="nuoG"
                     /locus_tag="J1Y83_002934"
                     /EC_number="1.6.5.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311194.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoG"
                     /protein_id="HBA7631065.1"
                     /translation="MATIHVDGKEYEVNGADNLLEACLSLGLDIPYFCWHPALGSVGA
                     CRQCAVKQYQNAEDTRGRLVMSCMTPASDGTFISIDDEEAKQFRESVVEWLMTNHPHD
                     CPVCEEGGNCHLQDMTVMTGHSFRRYRFTKRTHRNQDLGPFISHEMNRCIACYRCVRY
                     YKDYADGTDLGVYGAHDNVYFGRPEDGTLESEFSGNLVEICPTGVFTDKTHSERYNRK
                     WDMQFAPSICQQCSIGCNISPGERYGELRRIENRYNGTVNHYFLCDRGRFGYGYVNLK
                     DRPRQPVQRRGDDFITLNAEQAMQGAADILRQSKKVIGIGSPRASVESNFALRELVGE
                     ENFYTGIAHGEQERLQLALKVLREGGIYTPALREIESYDAVLVLGEDVTQTGARVALA
                     VRQAVKGKAREMAAAQKVADWQIAAILNIGQRAKHPLFVTNVDDTRLDDIAAWTYRAP
                     VEDQARLGFAIAHALDNSAPAVDGIEPELQSKIDVIVQALAGAKKPLIISGTNAGSAE
                     VIQAAANVAKALKGRGADVGITMIARSVNSMGLGIMGGGSLEEALTELETGRADAVVV
                     LENDLHRHASATRVNAALAKAPLVMVVDHQRTAIMENAHLVLSAASFAESDGTVINNE
                     GRAQRFFQVYDPAYYDSKTVMLESWRWLHSLHSTLLSREVDWTQLDHVIDAVVAKIPE
                     LAGIKDAAPDATFRIRGQKLAREPHRYSGRTAMRANISVHEPRQPQDIDTMFTFSMEG
                     NNQPTAHRSQVPFAWAPGWNSPQAWNKFQDEVGGKLRFGDPGVRLFETSENGLDYFTS
                     VPARFQPQDGKWRIAPYYHLFGSDELSQRAPVFQSRMPQPYIKLNPADAAKLGVNAGT
                     RVSFSYDGNMVTLPVEISEGLTAGQVGLPMGMSGIAPVLAGAHLEDLKEAQQ"
     gene            complement(11738..13075)
                     /gene="nuoF"
                     /locus_tag="J1Y83_002935"
     CDS             complement(11738..13075)
                     /gene="nuoF"
                     /locus_tag="J1Y83_002935"
                     /EC_number="1.6.5.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HBA7631066.1"
                     /translation="MKNIIRTPETHPLTWRLRDDKQPVWLDEYRSKNGYEGARKALTG
                     LSPDEIVNQVKDSGLKGRGGAGFSTGLKWSLMPKDESMNIRYLLCNADEMEPGTYKDR
                     LLMEQLPHLLVEGMLISAFALKAYRGYIFLRGEYIEAAVNLRRAIAEATEAGLLGKNI
                     MGTGFDFELFVHTGAGRYICGEETALINSLEGRRANPRSKPPFPATSGAWGKPTCVNN
                     VETLCNVPAILANGVEWYQNISKSKDAGTKLMGFSGRVKNPGLWELPFGTTAREILED
                     YAGGMRDGLKFKAWQPGGAGTDFLTEAHLDLPMEFESIGKAGSRLGTALAMAVDHEIN
                     MVSLVRNLEEFFARESCGWCTPCRDGLPWSVKILRALERGEGQPGDIETLEQLCRFLG
                     PGKTFCAHAPGAVEPLQSAIKYFREEFEAGIKQPFSNTHLINGIQPNLLKERW"
     gene            complement(13072..13572)
                     /gene="nuoE"
                     /locus_tag="J1Y83_002936"
     CDS             complement(13072..13572)
                     /gene="nuoE"
                     /locus_tag="J1Y83_002936"
                     /EC_number="1.6.5.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012124159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HBA7631067.1"
                     /translation="MHENQQPQTEAFELSAAEREAIEHEMHHYEDPRAASIEALKIVQ
                     KQRGWVPDGAIHAIADVLGIPASDVEGVATFYSQIFRQPVGRHVIRYCDSVVCHINGY
                     QGIQAALEKKLNIKPGQTTFDGRFTLLPTCCLGNCDKGPNMMIDEDTHAHLTPEAIPE
                     LLERYK"
     gene            complement(13575..15377)
                     /gene="nuoC"
                     /locus_tag="J1Y83_002937"
     CDS             complement(13575..15377)
                     /gene="nuoC"
                     /locus_tag="J1Y83_002937"
                     /EC_number="1.6.5.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C/D"
                     /protein_id="HBA7631068.1"
                     /translation="MVNNMTDLTAQEPAWQTRDHLDDPVIGELRNRFGPDAFTVQATR
                     TGVPVVWIKREQLLEVGDFLKKLPKPYVMLFDLHGMDERLRTHREGLPAADFSVFYHL
                     ISIDRNRDIMLKVALAENDLHVPTFTKLFPNANWYERETWDLFGITFDGHPNLRRIMM
                     PQTWKGHPLRKDYPARATEFSPFELTKAKQDLEMEALTFKPEEWGMKRGTENEDFMFL
                     NLGPNHPSAHGAFRIVLQLDGEEIVDCVPDIGYHHRGAEKMGERQSWHSYIPYTDRIE
                     YLGGCVNEMPYVLAVEKLAGITVPDRVNVIRVMLSELFRINSHLLYISTFIQDVGAMT
                     PVFFAFTDRQKIYDLVEAITGFRMHPAWFRIGGVAHDLPRGWDRLLREFLDWMPKRLA
                     SYEKAALQNTILKGRSQGVAAYGAKEALEWGTTGAGLRATGIDFDVRKARPYSGYENF
                     DFEIPVGGGVSDCYTRVMLKVEELRQSLRILEQCLNNMPEGPFKADHPLTTPPPKERT
                     LQHIETLITHFLQVSWGPVMPANESFQMIEATKGINSYYLTSDGSTMSYRTRIRTPSY
                     AHLQQIPAAIRGSLVSDLIVYLGSIDFVMSDVDR"
     gene            complement(15471..16133)
                     /locus_tag="J1Y83_002938"
     CDS             complement(15471..16133)
                     /locus_tag="J1Y83_002938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017490251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B"
                     /protein_id="HBA7631069.1"
                     /translation="MDYTLTRIDPNGENDRYPLQKQEIVTDPLEQEVNKNVFMGKLND
                     MVNWGRKNSIWPYNFGLSCCYVEMVTSFTAVHDVARFGAEVLRASPRQADLMVVAGTC
                     FTKMAPVIQRLYDQMLEPKWVISMGACANSGGMYDIYSVVQGVDKFIPVDVYIPGCPP
                     RPEAYMQALMLLQESIGKERRPLSWVVGDQGVYRANMQSERERKRGERIAVTNLRTPD
                     EI"
     gene            complement(16149..16592)
                     /gene="nuoA"
                     /locus_tag="J1Y83_002939"
     CDS             complement(16149..16592)
                     /gene="nuoA"
                     /locus_tag="J1Y83_002939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoA"
                     /protein_id="HBA7631070.1"
                     /translation="MSMSTSTEVIAHHWAFAIFLIVAIGLCCLMLVGGWFLGGRARAR
                     SKNVPFESGIDSVGSARLRLSAKFYLVAMFFVIFDVEALYLFAWSTSIRESGWVGFVE
                     AAIFIFVLLAGLVYLVRIGALDWTPARSRRERMNPETNSIANRQR"
     gene            complement(17222..18160)
                     /gene="lrhA"
                     /locus_tag="J1Y83_002940"
     CDS             complement(17222..18160)
                     /gene="lrhA"
                     /locus_tag="J1Y83_002940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LrhA"
                     /protein_id="HBA7631071.1"
                     /translation="MISANRPIINLDLDLLRTFVAVADLNTFAAAAAAVCRTQSAVSQ
                     QMQRLEQLVGKELFARHGRNKLLTEHGIQLLGYARKILRFNDEACSSLMFSNLQGVLT
                     IGASDESADTILPFLLNRVSSVYPKLALDVRVKRNAYMAEMLESQEVDLMVTTHRPST
                     FKALNLRTSPTHWYCAAEYVLQKGEPIPLVLLDDPSPFRDMVLATLNKADIPWRLAYV
                     ASTLPAVRAAVKAGLGVTARPVEMMSPDLRVLSGVDGLPPLPDTEYLLCYDPSSNNEL
                     AQVIYQAMESYHNPWQYSPMSAPEGDDSLLIERDIE"
BASE COUNT         4650 a         5128 c         4798 g         4001 t
ORIGIN      
        1 gaatgcatta ccgccaacgc cccgacacgc tagtcctgag caattagaaa aaacagttcg
       61 ttatcttaca caaactgtgc atccacgtag cgccgacaat attgataatc tgaataggtc
      121 cgcagagtat ataaaagagg tctttatcag tagcggtgcc agagttaccg cgcaggatgt
      181 ccccattacg ggtggcccct acaaaaacat tgttgctaat tatggtcctg ccgatggacc
      241 gctgattatt attggtgcgc actatgactc tgtcagtagt tatgaaaacg atcaattgac
      301 ctatacgcct ggcgcggatg ataacgccag cggcgtggca ggattactcg aactggcacg
      361 tttgttacag cagcagactc cagggattgg cgtccaactg gtcgcctatg cgtcggaaga
      421 accgcccttc tttcgtagcg atgaaatggg gagcgcggta catgcagctt cgcttgagcg
      481 tccagtgaaa ttaatgatag cgctggagat gattggttat tacgactcaa cgcctggtag
      541 ccaggattac ccatccccgg caatgtcctg gctttatccc gatcggggag actttattgc
      601 cgtggtcggc agaatgcagg atatcaacgc cgttcgtcag gtaaaagcgg cattgttgtc
      661 atcccgggat ttatctgttt attctatgaa tgccccaggt tttattcccg gtattgattt
      721 ctctgatcac cttaattatt ggcaacacga tattcccgcc gtaatgatta ctgacaccgc
      781 tttttatcgt aataaacaat accacttgcc cggtgatatc gcagacagat taaattatca
      841 gaaaatggct caggtagtgg atggagttac aaccttgtta tacaacagta aataataaaa
      901 atttctcgag gcggatccag aaaaagcaac atgcgcattt tattttcttt cgtctgtttt
      961 aagatgcgaa acatgaaatt caacggagga aaaataaatg ggaatgattg gctattttgc
     1021 agaaattgac tcagaaaaga ttaaccaact gcttgagtct actaaaaaac ctttgatgga
     1081 taatatacat gacacgcttt caggattacg ccgactggat attgataaac gctgggattt
     1141 tttacacttt ggcctgacgg gtacatctgc atttgacccc gcgaagaatg acccgctaag
     1201 tcgcgcagtg cttggtgaac acagcctaga agatggcata gatggcttcc tgggattgac
     1261 ctggaatcag gagttagccg cgacaatcga ccgactggaa tcactggatc gaagtgagct
     1321 gcgtaaacag ttttccatta agcgactcaa tgaaatggag atttatccgg gagtaacctt
     1381 ttcagaagag ttagaggggc aactctttgc ttcgatcatg cttgatatgg aaaagctgat
     1441 tagcgcgtat cgacgtatgc tgcgacaagg gaatcatgcg ctgacggtta ttgttgggtg
     1501 atagtgtcag ggcaccaaaa aaaaccgccg aacatgtcgg cggctttctg acttacaaac
     1561 tgactgctta catcagcggc attgctaacc gcacaatgct aatcagcggt tgtggccata
     1621 cacccagcac cagtaccaac agtgcagaga tcagcaccac gataccgcct gcgctgtact
     1681 gccagtttga tggtgcatcg cgacccggtt gttccggagc gtgaaggtat aagctcaccg
     1741 ccacgcgcag gtagtagtag aggccgattg ccgaaccgac aactacggca cccaccagcc
     1801 accacaagtg tgcctggaca ccgaccgcca gcacgtagaa cttaccgata aagcccagcg
     1861 tcattgggat accagccagt gaaagcatca tcaccgtcat cactgccgcg aggatcggac
     1921 gatgccagaa cagaccgcgg taagagaaca gggaatcagc atccgggcca cgatacgggc
     1981 tggacatcag gctgaccacg ccgaacgcgc cgaggctgct gaacagataa ccgaccaggt
     2041 aaacccctac cgcttccatc gacatctcgc cggtttgcag cgcaatcagc gccaccagca
     2101 gatagccgag gtgagagata gatgagtaac cgagcagacg tttgatattg gtctggctca
     2161 gcgccatcag gttaccgaag atgatagagg caaaggcgat aatcgccagc actacgcgaa
     2221 tcgcttcact gtcacccacc ggtgcgtaaa ggaacaaacg catgaccacg ccgaagatag
     2281 cgattttgct cgccgtcgcc aggaaagtgg aaaccggcgc aggcgcgccc tggtatacgt
     2341 ctggcgtcca caggtggaac ggcaccagag agagtttgaa accgaggcca acaatcatca
     2401 ggccaaaacc tgccagcaac agcggctcgt tgagcatacc gtcgccgagg tttttaccca
     2461 gtgcgacaaa cgacaggtcg ccagactgcg catacaccag cgccataccg aacagcagga
     2521 aagaagacgc tgcggcagaa aggatggtgt atttgatact ggcttccagc gaacgtttct
     2581 ggcggaaagc gtaaccgacc aggccaaaca gcggcaaaga gatcagttca ataccgagga
     2641 acagagacgc cagatggttg gcattcgcca gcaggatccc gcccagcgcg gcaattaaca
     2701 ccaacaggta gaactcgtcc ttgttgtcgt tatagccttc aagccacggg taggcgaaag
     2761 tacaggtggc gaggctcgcc aacaatacca gcccggtgta aagcatggcg aaaccatcaa
     2821 cgcgcatcag cggcgtaacg tccatagcgc ccgcctggcc gacaaaccag agcgatacca
     2881 gcgccgcgtt aagcccaata accgagagcg tagcgttgag gaaatgattg cgtcgccacg
     2941 caatggagag catcacaacc accaccgtca agccgacgat cagcaacggt agcagtgcga
     3001 tcaggttttg tggagttatt gtcatggcga tttacggcct tgtagtagta acggaattaa
     3061 caaaccactg ctggatattg ccaatcgcgg agtgcgaggt atccagaatc ggctgcggat
     3121 agaagcccag cagtaccagc agcaccacca gcaacaggat cataaacagc tcacgcagcg
     3181 acatccctgg cagttcctgg ctggcaatct ggcttttcgc tttaccgaag taagcgcgat
     3241 gcagcatcgc cagcgaataa acagaagcaa agaccagccc aaaggtagag ataacggtaa
     3301 tcaccgggac aacctggaag ctgccgaaca gaatcataaa ttcgccgacg aagttaccgg
     3361 tgccaggcat cccaagcgtt gccaccgcaa agaacagcga cagtgctggc agccatttca
     3421 ttttgctcca cagaccgccc atcatgcgca tgtcgcgggt atggatacgt tcataaagct
     3481 gaccacaaag aataaacaga cccgccgccg acaagccgtg cgcaatcatc tggattaccg
     3541 cgccctggta ggccaactgg ctgccggtgt agatagcaat cagcacgaag cccatgtggg
     3601 aaaccgaggt gtaggcgatc agacgtttga tatcggtctg ggcgaaggcc atccacgcac
     3661 catagaagat gccgataaca cccagccaca tagcgattgg cgcgaactct gccgacgcgt
     3721 tcgggaacag cggtagggag aaacgcagca gaccgtaagc ggcggttttc agcaagatcc
     3781 ccgcgaggtc aacggaaccg gcggtcggtg cctgggagtg cgcatccggt aaccagccat
     3841 gcagcggaac caccggcatt ttcactgcga aggcgatgaa gaagcccagc atcaacaggt
     3901 attctacgcc gttagacatc ggcgtattca gcagctcttc atagttgaag gtccagacgc
     3961 cggtcgcatt gtagtgaacg aaaaccagcg ccaggatggc gatcaacatc accagaccac
     4021 tcgcctgggt gtaaatgaag aacttggttg ccgccgtgat acgcgtttta ccgtcagagg
     4081 ctttatgccc ccacagtgcg atcaggaagt acatcggcac cagcatcatt tcccagaaga
     4141 agaagaacag gaacatgtcg atggcaagga acacgccgat aacgccgccc aggatccaca
     4201 tcaggttgag gtggaagaag ccctgatatt tttcgatctc tttccacgaa cagagtaccg
     4261 ccagcacacc gagcagaccg gtcagcacga ccatcagcaa cgacagaccg tcaatggcga
     4321 gatgaataga gataccaaaa cgcgggatcc acggcatgtc gaattcagac tgccactgcg
     4381 gaattccggc ggattgcgtc agtgaataac cgccctgcaa ccacagttgc agcgacagcg
     4441 ccagcgtcaa tcccatggtt accagcgcga tccagcgcgg caccttgacg ccaaagcgtt
     4501 cggtctgcca gcacaggaag ccgccgataa agggaattaa tattagccag ggtagtaaca
     4561 tggcgatctt tattccttgt aaaagtcccg tcaggaccgg attttcaacg aattctcacg
     4621 acagaaaatt ctcttcggac tgggggtagt gtcggatgcg ccgcgaagac ggcttatccg
     4681 acctacgttc ttatatgccc aggggcaatc ccacaatcct taactcaacg cagtaccatc
     4741 aacagtgcca gcaccacgac cgcaccgatg ctcatggatg ccacatacca gcgcagataa
     4801 ccgttctcgc ttaacagcag acctttacct gcaaagcggg aaaggacagc cgggatgttc
     4861 atcattgagt tcagcggatc gcgtttcagc aaccaggcaa tacccaggaa cggcttgacg
     4921 aacactttgt cgtacagcca gtcaaagccc caggcgttgt accaccaggt acccagcaga
     4981 cggcccggcg cactgttggc gatggaggtc accagagtac gtttacccag ccacagccag
     5041 gcagccagca gaatgccgac caccgcgacc acgccagagg taatttccag ggtcaacatg
     5101 ctgccgtgcg ccagttccgt cgtttgcgga agcacgccct gcagcggcgg tacaatcagt
     5161 gcgccaacga aggtggaaag gatcagcagc acaatcagcg gcaggctgtg agttacccct
     5221 ttcacggcgt gagcgtgaat ttgttctttt ccgtggaaga cgatgaaaat catacggaag
     5281 gtgtaaagcg aggtcataaa tgcaccgacc agacctgcca ccatcagatt gatatgtcca
     5341 ttcgccatcg cacccgcgag gatctcatcc ttactgaaga agcccgcagt gaccagcggc
     5401 agtgccgaca gtgctgcgcc gcccaccagg aagcagagat aaaccagcgg aatagattta
     5461 cgcagaccac ccatcttaaa gatgttctgt tcgtgatggc aggccagaat gacggaaccg
     5521 gatgccagga acagcagcgc tttaaagaac gcgtgggtca tcaagtggaa aatcgccgca
     5581 tcccatgcct gcacgccaag cgcgaggaac atgtagccaa tctggctcat ggtagagtaa
     5641 gcgagaacac gtttgatgtc ggtctgtacc agcgccgcaa aaccggccag cagcagcgta
     5701 accgccccaa caatgcccac cagatgcaga acttccggcg tcatcaggaa caggccgtgg
     5761 gtacgggcga tcaggtagac acccgcggtt accattgttg cagcgtggat cagcgcggag
     5821 acaggcgtcg ggcccgccat tgcgtcggca agccatgtct gcaacggcaa ctgagcagat
     5881 ttaccgaccg caccgcccag cagcatcagc gtcgcccaca tcagcatgtt attgccgtca
     5941 gcaaagtgcg ctggtgccag ttccaccatt tcgcggaagt tcagggtgcc cagttcgttg
     6001 taaagaatga acagcgcgaa agcgaggaag acgtcaccca cacgggtcac gacgaacgct
     6061 ttcattgccg ctgcgccatt cttcggatcg gtgtaataga aaccgatcag cagataggag
     6121 cacaggccca cgccttccca gccgaggtac atcagcagca ggttgtcggc aagcaccaga
     6181 actaccatgc tggcgatgaa caggttggtg taagcgaaga agcgagagta gccctcttca
     6241 ccgcgcatat accaggaggc gtacatgtga ataaggaaac ccacaccagt gaccaccgag
     6301 agcatggtca gcgacaggcc gtccagcacc aggttaaaac cgatgttaaa gtcgcctacc
     6361 gacatccacg tccacagcgg ctggctgtat gcctgctcac cgttagcgaa gaagtcaacg
     6421 ccgataaagg cggttaccag cgccgccagg cccacagagc caacgccgac gatcgccgag
     6481 acgttttcag accagcgccc acgggagaac gccagcagga cgaagccaat caatggcaaa
     6541 ataatggtta aggcaagcat gttcatccgc gcatctcact tactgaatcg atgttcaggt
     6601 tctggcgacg acggtgaagt tgcagcagca gcgcaaggcc gatactcgct tccgccgccg
     6661 cgaggctgat ggcgagaatg tacatcacct gaccgtcggt ctggccccag tagcttccgg
     6721 cgaccacaaa ggccaacgca gaggcgttaa tcatgatttc cagaccaatc agcataaaca
     6781 gcagattgcg acggataacc agaccggtta agccaagaac gaataagatt gccgcgagga
     6841 tcagtccatg ttgtaagggg atcatgcgtg ctcctccgtt tttcttttcg cgctgtcgtc
     6901 tttacgattg ctcagcactt cacccgcacg ctcttcacga ccgacgtgga aggccacaac
     6961 cagacccgcg agcagcagca tagaagccag ttccaccgcc agtacgtaag gcccgaacag
     7021 cgtaataccc actgctttag cactgattgg cgtaccgtcg ataccttgat cgttaacacc
     7081 gagaatggcg taaacaatca ccaccagcat gatggctgac aaaattgccg gaccaatcca
     7141 cacctgcggt ttcagccact ggcgttcctg ttcgatttct gaaccgccca ggttgagcat
     7201 catcaccacg aacacgaaca gcaccataat ggcacccgca tagacgataa tttccagcgc
     7261 accggcgaag taagcgccca gtgagaagaa caccccggaa atcgccagca gcgaaataat
     7321 caggtacagc agcgcgtgta ccggattggt atgggtgatc actcgcaagg tcgcaagtat
     7381 ggctatcagg ccacagatat aaaaagcgaa ctccattgcc cctctcctta cggtaacagg
     7441 ctcttgacgt cgataggctt ggcttcgttc tctgcttcgc ccttatcttt gccgtcgatt
     7501 gccatacctg ccatccggta gaagttatat tccgggtatt tgcccggacc ggagatcagc
     7561 agatcctctt tctcgtaaac cagatcctgg cgcttgtatt cccccatttc gaaatccggg
     7621 gttaactgaa tcgccgtggt cggacaggct tcttcgcaca gaccacagaa aatgcagcgt
     7681 gagaagttga tgcggaaaaa ttccggatac cagcgaccgt ctttggtttc tgctttttgc
     7741 agcgagatac agccgaccgg gcaggctacc gcgcagaggt tacaggctac gcaacgctct
     7801 tcgccgtccg gatcgcgggt cagaacgata cgaccacgat aacggggcgg cagatagacc
     7861 ggctcttccg ggtacattcg cgtttcgcgt ttggcgaacg cgtgcaggcc gatcatccag
     7921 atactacgaa cctgggtgcc gaaacctact aacaattctt ttaaggtcat ggtcttattg
     7981 ccccttattg cgcctgccag agaatgacag ccgccgttac cagcaagttg atcagcgtca
     8041 gcggcaggca gattttccag ccgaaggaca ttacctggtc ataacgcgga cgcggtaacg
     8101 acgcacgaat caaaatgaac atcatcataa agaacgcggt tttcagcgcg aaccagatga
     8161 atggcggtaa caacgggcct tgccagccac cgaagaacag cgtcaccatc agtgcagaga
     8221 tggtcacaat cccgatgtat tcgcccacga agaacagacc gaacttcata ccggaatatt
     8281 caatgtggta accatccgcc agttcctgct cggcttccgg ctggtcaaac gggtgacggt
     8341 gacacaccgc cacgcccgcg atggcaaagg taataaaacc aaagaattgc gggatgacgt
     8401 tccacacatg cgcctggctg ttgacgatgt cggtcatgtt gaatgaaccg gcctgcgcca
     8461 ccacgcccat cagggaaagc ccgaggaaca cttcgtagct cagggtctgc gcagaagcac
     8521 gcatcgcacc cagcagcgag tatttgttgt tacttgacca gcccgcaaac agcaccgcgt
     8581 aaaccgccag acctgccatc atcaggaaga acaaaatccc gatgttcagg tcggcaacca
     8641 cccaacccgg actgactggc acaattgcaa aggccagcag cagcgaggta aaggcaatca
     8701 tcggtgctag agtaaagatg acgcgatccg agaatttcgg gatccagtct tctttaaaga
     8761 acattttgat catgtccgca accagctgga gcgaaccgcc ccagccaaca cggttaggtc
     8821 cataacggtt ctggaacaga cccagcaggc gacgttcgcc aaagctcatg aatgccccac
     8881 aggtgactac caccagcagg atcaccaccg ctttaaggac ggtcagcagg atctcaatca
     8941 gttccggtga tatccaactc attgttgtgc ctccttgaga tcctcaagat gcgcgccagc
     9001 cagcaccgga gcaatgccgg acatacccat cggcaagccc acctgccctg ccgtcagccc
     9061 ttcggagatt tcaaccggca gcgtgaccat gttgccatcg taactaaagg agacgcgtgt
     9121 acctgcgttc acccccaact tcgcggcatc cgctgggttg agtttgatgt acggctgcgg
     9181 catacggctc tggaagaccg gagcacgctg tgacaattca tcgctgccaa acaggtggta
     9241 gtacggcgcg atacgccatt tcccgtcctg cggctggaag cgtgccggta cgctggtgaa
     9301 gtaatccaga ccgttttcgc tggtttcaaa cagacgcacg cccggatcgc caaaacgcag
     9361 tttgccgccc acttcgtcct ggaatttgtt ccacgcctgc ggggagttcc agcctggcgc
     9421 ccaggcaaac ggcacttgcg aacggtgcgc ggtcggctgg ttgttacctt ccatcgagaa
     9481 ggtgaacatg gtgtcaatat cctgcggctg acgcggctca tgaacgctga tgttggcgcg
     9541 catggcggta cgaccgctgt aacggtgcgg ctcacgggcc agtttctgcc cacgaatgcg
     9601 gaatgtcgca tccggcgcag catctttgat acctgccagt tccgggattt tcgccacaac
     9661 agcgtcaatt acatggtcga gctgcgtcca gtccacttca cggctcagca gggtgctgtg
     9721 cagcgagtgt aaccagcgcc agctttccag catgacagtt ttgctgtcgt aataagccgg
     9781 atcgtaaacc tggaagaaac gttgggcgcg gccttcgttg ttgatcaccg taccgtcgct
     9841 ttcagcaaag ctggcagcag aaagcaccag atgggcgttt tccatgatcg ctgtgcgttg
     9901 atggtcaacc accatcacca gcggcgcttt agccagcgca gcattcacgc gggtagcaga
     9961 agcgtgacga tgcagatcgt tttccagcac caccaccgcg tcggcgcgtc cggtttccag
    10021 ttcagttaac gcttcttcaa gcgaaccgcc acccataatg cccagcccca tgctgtttac
    10081 agagcgggca atcatggtga taccgacgtc agcgccgcga cctttcaggg ctttcgccac
    10141 gttagccgca gcctgaatca cttcagcgct accagcgttc gtcccggaga taatcaacgg
    10201 tttcttcgca ccagccagtg cctgcacgat gacgtcgatt ttgctttgca gctcaggttc
    10261 gataccgtca accgctggcg cggagttatc cagcgcatgg gcgatggcaa aacctaaacg
    10321 cgcctgatct tcaaccggtg cgcggtaagt ccacgcagcg atatcatcca gacgggtgtc
    10381 atcaacgttg gtaacaaaca gtggatgctt cgcacgttga ccgatgttga ggattgccgc
    10441 aatctgccag tcagccactt tctgtgctgc cgccatttcg cgtgctttac cttttaccgc
    10501 ctggcgcacc gccagcgcaa cgcgcgcgcc ggtctgggta acgtcttcgc ccagcaccag
    10561 taccgcatcg taagattcga tttcgcgcag agccggagta taaatgccgc cttcacgcag
    10621 tactttcagc gccagttgca gacgttcctg ctctccgtga gcgataccgg tatagaagtt
    10681 ttcttcgccc accagctcac gcagcgcaaa gttgctttcc acgctggcac gcggagaacc
    10741 aataccgatc actttcttcg actgacgcag aatatctgct gcaccctgca ttgcctgttc
    10801 ggcgttgagg gtaatgaaat catcgccacg acgctgtact ggctgacgcg gacgatcttt
    10861 caggttgacg taaccgtaac cgaaacgacc acggtcgcag aggaagtagt ggtttaccgt
    10921 accgttgtaa cggttttcga tacgacgcag ttcgccataa cgctcgccgg ggctgatgtt
    10981 acagccgatg gaacattgct ggcagatgct cggcgcaaac tgcatatccc atttacggtt
    11041 gtaacgctcg gagtgcgttt tgtcggtgaa tacaccggtc gggcaaattt cgaccaggtt
    11101 accggaaaat tcgctttcca gcgtgccgtc ttccgggcga ccgaagtaga cgttgtcgtg
    11161 cgcaccgtaa acgcccaggt ccgtgccgtc agcgtaatct ttgtagtaac gcacgcagcg
    11221 gtagcaggcg atgcagcggt tcatttcgtg agagatgaat ggccccaaat cctgattacg
    11281 gtgggtacgt ttggtgaaac ggtagcgacg gaagctatgt ccggtcatca cagtcatatc
    11341 ctgaagatgg cagttaccgc cctcttcaca taccggacag tcgtgcgggt ggttggtcat
    11401 caaccactca accacgcttt cacggaactg tttcgcttct tcgtcgtcaa tggaaataaa
    11461 ggtgccatcg gaagccggtg tcatacagga catcaccagg cgaccacgcg tgtcttccgc
    11521 gttttggtat tgcttcaccg cacactggcg gcaagcaccg acgcttccca gcgccggatg
    11581 ccagcaaaag taaggaatat caaggcccag agacagacaa gcttctagca ggttgtccgc
    11641 tccgttgacc tcgtattctt tgccgtctac atgaattgta gccattagca tgcttccagt
    11701 tttctcagtc agagactgag cgttaatcga aattcggtta ccagcgctct ttcagcaggt
    11761 ttggctgaat cccattaatc aaatgggtat tgctgaacgg ctgtttgatt cccgcctcaa
    11821 attcttcgcg gaaatatttg atggcgctct gtaacggctc cactgcacca ggtgcgtggg
    11881 cacagaaagt tttacccggg cctaagaatc gacacagttg ctcaagtgtt tcgatatcgc
    11941 ccggctggcc ttcgccacgc tccagcgcac gcagaatttt cacgctccac ggcagaccgt
    12001 cgcggcatgg cgtacaccag ccgcaggact cacgggcgaa aaactcttcc aggttacgca
    12061 ccagcgacac catgttgatc tcgtggtcaa ccgccatcgc cagcgccgta cccagacggc
    12121 ttcccgcttt accgatactt tcgaattcca tcggcaggtc aaggtgcgct tcggtcagaa
    12181 agtcagtccc cgcgccgcct ggctgccagg ctttgaattt cagaccgtca cgcataccac
    12241 cggcgtagtc ttcgaggatc tcgcgtgcgg tggtaccgaa cggcagttcc cacagacccg
    12301 gatttttcac ccgaccagag aagcccatca gcttggtgcc agcatcttta cttttcgaga
    12361 tgttctgata ccactccacg ccgttagcga ggatcgccgg aacgttacac agggtttcaa
    12421 cgttgttaac gcaggttggt ttaccccatg caccggaggt tgccgggaag ggcggtttcg
    12481 agcgtgggtt agcgcgacgc ccttccaggg agttgattaa cgctgtttct tcaccgcaga
    12541 tgtagcgccc tgccccggta tggacgaaca gttcgaaatc gaaacctgtt cccataatgt
    12601 ttttgccaag cagacccgct tcggtggctt cggcaatggc acggcgtaga ttcactgccg
    12661 cttcgatata ttcgccgcgc aggaagatat agccacggta agctttcagc gcaaacgcgg
    12721 agatgagcat gccttccacc agcaggtgcg gcagttgctc catcagcaga cggtctttat
    12781 aggtgcccgg ctccatttca tcggcgttac acagtaggta acggatattc atggattcgt
    12841 ctttcggcat caggctccac ttcagacctg tcgaaaagcc cgcaccgccg cgccctttca
    12901 gaccagagtc ttttacctga ttaacgattt cgtccggaga caacccggtc agcgccttac
    12961 gcgcgccttc gtaaccgttt ttgctgcgat attcgtccag ccacactggc tgtttgtcat
    13021 cgcgcagacg ccaggtcagc ggatgcgttt cgggagtacg gataatgttt ttcatttata
    13081 ccgctccagc agttcaggga tcgcttccgg ggtcagatgc gcgtgagtgt cctcatcgat
    13141 catcatgttt ggccctttat cacagttccc caggcagcaa gttggcagca gcgtaaagcg
    13201 gccatcaaat gtcgtttgcc ctggtttgat gttcagcttt ttctcgagcg ccgcctgaat
    13261 accctggtaa ccgttgatat gacagaccac gctgtcgcaa taacggatca catggcggcc
    13321 tactggctga cggaagatct ggctataaaa cgttgccacg ccttcgacat cgctcgccgg
    13381 aatacccagc acatcggcga tcgcgtggat cgcgccatcc ggcacccagc cacgctgctt
    13441 ctgaacgatt ttcagcgctt caatggacgc cgcacgcggg tcttcgtagt ggtgcatctc
    13501 gtgctcaatc gcttcacgct ctgccgcact cagctcaaaa gcctcggttt gtggttgttg
    13561 attctcgtgc ataattagcg gtccacatct gacataacaa aatcgatact gcccagataa
    13621 acaatcaggt cagacaccag gctgccgcgg atcgccgccg gaatttgctg caaatgcgca
    13681 taactcggcg tgcggatacg ggtgcgataa ctcattgtgc tgccgtcgct ggtcaggtag
    13741 taactgttga tccctttggt cgcctcaatc atctggaagg attcattggc aggcatcacc
    13801 ggcccccacg acacttgcag gaagtgggtg atcagggttt cgatatgttg caacgtgcgc
    13861 tctttcggcg gcggcgtggt cagcgggtga tccgctttga acgggccttc cggcatgttg
    13921 ttgaggcact gctcaagaat gcgcagactc tggcgcagct cttccacttt cagcattacg
    13981 cgggtgtagc agtcagaaac gccaccaccc accgggattt caaagtcgaa gttttcatag
    14041 ccagaataag gacgcgcctt acgcacgtcg aagtcgatcc cggtggcacg caggcccgcg
    14101 ccggtggtgc cccactccag cgcctctttc gcgccgtaag cggcaacgcc ctgggaacga
    14161 cctttcagaa tggtgttttg cagcgccgct ttctcgtaag acgccagacg tttcggcatc
    14221 cagtcgagga actcacgcag caggcgatcc cagccgcgcg gcaggtcgtg cgctacgccg
    14281 ccaatacgga accacgccgg gtgcatacgg aaaccagtga ttgcttccac cagatcgtaa
    14341 attttctgac gatcggtaaa ggcgaagaac actggcgtca tcgcgccgac gtcctgaata
    14401 aaggtggaga tatacagcag gtgactgttg atgcggaaca gttcggagag cattacgcga
    14461 atgacgttaa cgcgatccgg cacggtgatc ccagccagtt tctctaccgc cagcacataa
    14521 ggcatttcgt taacgcagcc gccgaggtat tcgatacggt cggtatacgg aatgtagctg
    14581 tgccaggact gacgttcgcc cattttctca gcgccacggt ggtggtaacc gatgtctggt
    14641 acgcagtcga caatctcttc gccatcaagc tgcaaaacga tacggaaagc accgtgcgca
    14701 gacgggtggt tcggaccgag gttgaggaac atgaagtcct cattttcggt gccgcgcttc
    14761 atcccccact cttccggttt gaaggtcagg gcttccatct ccagatcctg tttggctttg
    14821 gtcagctcaa acggcgagaa ttcggtagcg cgcgccgggt aatctttacg cagcgggtga
    14881 cctttccagg tttgcggcat catgatgcgg cgcaggttcg ggtgaccgtc gaatgtaatg
    14941 ccaaatagat cccaggtttc acgctcatac cagttagcgt ttgggaataa tttggtaaag
    15001 gtcggtacgt gcaggtcgtt ttctgccagc gccaccttca gcatgatgtc gcggttacga
    15061 tcgatagaaa tcagatggta gaaaacggaa aaatccgcgg caggtaaccc ttcgcggtgt
    15121 gtgcgcagac gttcgtccat gccgtgtaag tcaaacagca tgacgtaagg tttcggcagt
    15181 ttctttaaga aatcgcccac ttccagtaat tgttcacgct tgatccacac aacgggaacc
    15241 ccggtgcgag tcgcctgaac agtaaaggca tccggcccaa aacggttgcg cagttcgcca
    15301 atcaccggat catcaagatg atcgcgggtc tgccaggcgg gttcttgcgc ggttaagtcg
    15361 gtcatattgt tcaccattgc aaatggtccg tggtgactgt cggcgcagta agcttcgcga
    15421 aatagattta ggaataagcg aagaaaattc cctttgccga caggcgcaaa ttaaatctcg
    15481 tcaggtgtac gcaggttggt aacggcaatg cgttcaccgc gcttgcgttc gcgctctgat
    15541 tgcatattgg cgcgataaac gccctgatcg ccaaccaccc aggagagcgg acgacgttct
    15601 ttaccgatag attcctgcaa cagcatcagt gcctgcatgt acgcttcagg acgcggcgga
    15661 cagcccggga tatacacatc aaccgggatg aatttatcaa cgccctgcac aacggaataa
    15721 atatcgtaca taccgccgga gttggcacag gcacccattg agataaccca ttttggttcc
    15781 agcatctggt catacaaacg ctgaataacc ggtgccattt tggtaaagca ggttcctgca
    15841 accaccatca ggtcagcctg acgcggcgaa gcacgcaata cttcagcgcc aaaacgcgcc
    15901 acgtcatgca ccgcggtaaa cgaagtcacc atctcaacgt aacagcagga aagaccgaag
    15961 ttatacggcc aaattgagtt tttacgaccc cagttaacca tgtcattgag cttgcccata
    16021 aacacgtttt tgttaacttc ttgctccaga gggtcggtta cgatctcctg cttttgcagg
    16081 gggtaacggt cattctcacc gttgggatct atgcgggtga gcgtataatc catcttaatg
    16141 cctcgcggtt agcgttgacg attagcgata ctgttcgttt ccgggttcat acgctcgcgg
    16201 cgtgaacgcg cgggcgtcca gtccagcgcg ccaatacgca ccagataaac cagacctgcc
    16261 agtaacacaa aaataaaaat tgcagcttcc acaaagccta cccagccgct ttcgcggata
    16321 gaggttgacc atgcgaacag atacagcgct tcaacgtcga agataacgaa gaacatggcc
    16381 accagataaa acttggcgga caggcgtaag cgggcggagc cgaccgagtc gataccggat
    16441 tcaaacggaa cgttttttga cctcgcgcgt gcgcgaccgc ctaaaaacca accgcctacc
    16501 agcatcaggc aacacaggcc aatggcaacg ataagaaaga tagcgaatgc ccagtgatga
    16561 gcgatgactt cagtggatgt tgacatactc attgcttact catcaaaagt agcgcctgat
    16621 tctctgctct ttacggcaga tggacgccac atcgattcat ggggaggaat aaaaaaaacc
    16681 ttacaataac tgcagaaatt cttttataca gctaattgat gtggtctttt actcctttct
    16741 ataacctttt gtcaacttta acaaaggttt cttcacactg atttacacaa tgacaacaac
    16801 atttgcattt ggcgcccttt ctccccagat cattgacgac tccaggaaaa ttagatgttg
    16861 ttgaatcgtg tccgttgtga agcaatggaa aaaatacggg tctattttga caggaatttg
    16921 tgtcgattcc tccccccaaa agagagtatt ttcttgatct gtgacacgct tttgtcattc
    16981 tataacaaaa atgcagcaac aaattcacgt attttttaac attattgtag caggagattt
    17041 ttttcaggtg attatttgtg cgtacggggc gtgaatctct ggtgattgaa aaatgaacag
    17101 ttttgtatgt tctgcacgat acgaggaagg ctttttacta aaaaaaagcc gctggggttt
    17161 aaaataaacc cagcggttcg tttttacact attgtctcag gaattatcta tcgtccgtcg
    17221 attactcgat atccctttca atcaacaatg agtcatctcc ttccggagca gacattgggc
    17281 tgtattgcca cggattatgg tagctctcca tcgcctgata gatcacctgg gccagctcat
    17341 tattactgga cgggtcatag cacagcaaat attcggtatc aggtagcggc ggtaagccat
    17401 ctactccact cagcacacgc aggtccggac tcatcatctc gaccggcctt gccgtcacgc
    17461 caagaccggc tttcactgcc gcacgaacgg ccggaagcgt cgatgcgaca taagccagtc
    17521 gccagggaat atctgcttta ttaagcgtcg ccagcaccat atcgcgaaac ggacttggat
    17581 catccagcaa cacaagaggg atcggctcac ctttttgcaa tacgtattcc gctgcgcagt
    17641 accagtgtgt tggcgaggta cgcaggttca atgccttaaa ggtagaagga cgatgggtgg
    17701 ttaccatcaa atccacttcc tgggattcca gcatttctgc catgtaggca ttacgtttaa
    17761 cgcggacatc cagcgctagt ttcggataaa ccgaactcac gcgatttaac aggaaaggta
    17821 agatcgtatc ggcagattca tctgaagcac cgatagttaa cacgccctga agattactga
    17881 acattaatga tgagcaggcc tcatcattaa aacgcaggat tttcctggcg taaccaagaa
    17941 gttgaatgcc atgttcagtt aacagtttgt tgcgaccgtg acgagcgaac agttctttcc
    18001 caacgagttg ttccagacgc tgcatttgct gacttacggc ggactgagta cgacacacag
    18061 cggcagctgc ggcagcaaaa gtgttcagat cggcaacagc aacaaatgtt ctcagcagat
    18121 cgaggtcgag gttaattatc ggacgatttg cacttatcat atattatcac ttactggcgg
    18181 ctcatactga gctggttaat gctgtgcaca cacaaacaag caattccatt tgtaatgtgc
    18241 ctccctggca gtttcatccg gaacccggac gaaagtaaaa atgcatatga gttgcactaa
    18301 aaaagtgact cacattgttc cgttataatg cctgaagtag atcacagaat atatcttcag
    18361 ggatcgcata tctattaagt tactcactct tttctattta tgacatgcgc gtgtttgtat
    18421 aaatgtaaat gtgagtcctt gttccactct cgtacagcat cgctggtcat acgcgaacac
    18481 gtacaaacag caatggtgag gcggcatcaa gagcagggat ccgtcattta tgcaagcatt
    18541 ttaccccaaa aaccttttat ttataagggt cattgcg
//
LOCUS       DADSYM010000100        18366 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2415
            SAMN08039579-rid11723173.denovo.100, whole genome shotgun sequence.
ACCESSION   DADSYM010000100 DADSYM010000000
VERSION     DADSYM010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039579
            Sequence Read Archive: SRR6362533
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18366)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18366)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980162.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 03:39:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,484
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 4,347
            CDSs (with protein)               :: 4,347
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 43 of 85
            Pseudo Genes (incomplete)         :: 37 of 85
            Pseudo Genes (internal stop)      :: 19 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18366
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039579-rid11723173.denovo.100"
                     /strain="LGE2415"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8302 N 95.9628 W"
                     /collection_date="2015-08-24"
     gene            48..149
                     /locus_tag="J1Y83_002941"
     CDS             48..149
                     /locus_tag="J1Y83_002941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA7631072.1"
                     /translation="MRTYNPNSLLHSQMQKCTCVFLHPASDLCGGEA"
     gene            146..601
                     /gene="ybcN"
                     /locus_tag="J1Y83_002942"
     CDS             146..601
                     /gene="ybcN"
                     /locus_tag="J1Y83_002942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA base-flipping protein YbcN"
                     /protein_id="HBA7631073.1"
                     /translation="MNLPQDGIKLHRGNFTAIGQQIQPYLEDGKCFRMVLKPWREKRS
                     LSQNALSHMWYSEISEYLISRGKTFATPAWVKDALKHTYLGYETKDLVDVVTGDITTI
                     QSLRHTSDLDTGEMYVFLCKVEAWAMNIGCHLTIPQSCEFQQLRDKQEA"
     gene            601..771
                     /locus_tag="J1Y83_002943"
     CDS             601..771
                     /locus_tag="J1Y83_002943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA7631074.1"
                     /translation="MATPLIRVMNGHIYRVPNRRKRKPELKPSEIPTLLGYTASLVDK
                     KWLRLAARRNHG"
     gene            764..1054
                     /locus_tag="J1Y83_002944"
     CDS             764..1054
                     /locus_tag="J1Y83_002944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1364 domain-containing protein"
                     /protein_id="HBA7631075.1"
                     /translation="MADLRKAARGRECQVRIPGVCNGNPETSVLAHIRLAGLCGTGIK
                     PPDLIATIACSACHDEIDRRTHFVDAEYAKECALEGMARTQVIWLKEGVIKA"
     gene            1051..1407
                     /locus_tag="J1Y83_002945"
     CDS             1051..1407
                     /locus_tag="J1Y83_002945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HBA7631076.1"
                     /translation="MNTYHITLPWPPSNNRYYRHNRGRTHISAEGQAYRDNVARIIKG
                     SMLDIGLAMPVKIRIECHMPDRRDLDNLQKAAFDALTKAGFWLDDELVVDYRVVKMPV
                     TKGGKLELTITELGNE"
     gene            1404..1544
                     /locus_tag="J1Y83_002946"
     CDS             1404..1544
                     /locus_tag="J1Y83_002946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlcG family protein"
                     /protein_id="HBA7631077.1"
                     /translation="MMFESYMAERLRRRWVRLRLYRFPGSVLTDYRILKNYAKTLKGA
                     AA"
     gene            1541..2230
                     /locus_tag="J1Y83_002947"
     CDS             1541..2230
                     /locus_tag="J1Y83_002947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HBA7631078.1"
                     /translation="MNTQYLQYVREQLIVATADLSGATKGQLEAWLEHAQFDTGTYKR
                     KKLRILDEVTGRMITLDNPPISGKQSYAKGSSIALVSQVEFSTSSWRRAVLSLEEHQK
                     AWLLWSYSESVRWEHQVTITQWAWSEFKTLLGTRKIAGKTLERLKKLIWLAAQDVKNE
                     LAGRKTYEYQELASLVGVTSKNWSETFTERWVAMKHIFLQLDSEALLLVTRTRSKQKT
                     TFSQQSIAKLD"
     gene            complement(2452..3255)
                     /locus_tag="J1Y83_002948"
     CDS             complement(2452..3255)
                     /locus_tag="J1Y83_002948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3800 domain-containing protein"
                     /protein_id="HBA7631079.1"
                     /translation="MTEKDNIGKLIWHIACDESGIDGQRFYGFGSLWMKYQRRGDFCQ
                     LIRELRSKHGFFEEIKWQKAHSKRYSEFYLELIDLFFRVPWLAFHCIVVEKSIVNKTF
                     HNGDYDLARRKHFTNLITTKISSVISAHPERDSYFRIEVDPIASRYKKADEELNVIAN
                     NILNRKFGRKGIISSVVTKDSKASENIQLADFFLGAVMCAYQGKASSEAKIRVSNYVA
                     SYLGWDHLQYDTWHTERKFNIWYFYDKTRGPRDIETQNVSLKYPLPQKK"
     gene            3484..3789
                     /locus_tag="J1Y83_002949"
     CDS             3484..3789
                     /locus_tag="J1Y83_002949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holin"
                     /protein_id="HBA7631080.1"
                     /translation="MHEKESLAGAFWLVLLIIAGWGGLVRYLIDVKQSKATWSWINAL
                     AQIVVSGFTGVIGGLISIESGFSIYMILATAGISGAMGSVALTYFWERLTGVKNAKS"
     gene            3776..4252
                     /locus_tag="J1Y83_002950"
     CDS             3776..4252
                     /locus_tag="J1Y83_002950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family protein"
                     /protein_id="HBA7631081.1"
                     /translation="MQNLNPQRKAFLDMLAWSEGTDNGRQPTRNRGYDVIVGGELFTD
                     YSDHPRKLVTLNPKLKSTAAGRYQLLSRWWDAYRKQLGLTDFSPKSQDAVALQQIKER
                     GALLMIDRGDIRQAIDCCSNIWASLPGAGYGQYEHKIGDLISRFKEAGGVVNEVEL"
     gene            4236..4628
                     /locus_tag="J1Y83_002951"
     CDS             4236..4628
                     /locus_tag="J1Y83_002951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000778467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2570 domain-containing protein"
                     /protein_id="HBA7631082.1"
                     /translation="MKLSYKLVIAAFLVTVIGSFIWSANHYYSKYQHEKKRADEAVQN
                     AKSATVITNNVLQSLQIVNTVLEASQHAKQQIALESQRTQTDIKTAVANDDCAARPVP
                     ARAAERLRRYADSLRTSSSGTATGESDH"
     gene            5089..5628
                     /locus_tag="J1Y83_002952"
     CDS             5089..5628
                     /locus_tag="J1Y83_002952"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA7631083.1"
                     /translation="MNADYIAYESLVATRNTAEWAFWSMVGTWFSGIATFMAVCLTLY
                     ISNRRPRPKLRGTVSLGGITGQNVSTFGVTINIANVGIQTATITSVTWTFGTKNSLLQ
                     ILGGGFGDDLPKKIEHGEYAFFFIQNDTNGDWARDMKNKIWEQGGNVRKLMLLVHLAT
                     GDTMKIKPAKNVIKMIEKS"
     gene            5736..6023
                     /locus_tag="J1Y83_002953"
     CDS             5736..6023
                     /locus_tag="J1Y83_002953"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA7631084.1"
                     /translation="MMTSLTVAVLALIVAIWHEINRFPATGKSLLSLQQEVRELRDEN
                     ERLASEVDSLKDELLEISNQIDRIKDPEYYALLDAGDGVGLYEIEKSRGQI"
     gene            6782..7327
                     /locus_tag="J1Y83_002954"
     CDS             6782..7327
                     /locus_tag="J1Y83_002954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein"
                     /protein_id="HBA7631085.1"
                     /translation="MEVNKKQLADIFGASIRTIQNWQEQGMPVLRGGGKGNEVLYDSA
                     AAIKWYAERDAEIENEKLRREVEELRQASETDLQPGTIEYERHRLTRAQADAQELKNA
                     RDSAEVVETAFCTFVLSRIAGEIASILDGIPLSVQRRFPELENRHVDFLKRDIIKAMN
                     KAAALDELIPGLLSEYIEQSG"
     gene            7302..9227
                     /locus_tag="J1Y83_002955"
     CDS             7302..9227
                     /locus_tag="J1Y83_002955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit family protein"
                     /protein_id="HBA7631086.1"
                     /translation="MNISNSQVNRLRHFVRAGLRSLFRPEPQTAVEWADANYYLPKES
                     AYQEGRWETLPFQRAIMNAMGSDYIREVNVVKSARVGYSKMLLGVYAYFIEHKQRNTL
                     IWLPTDGDAENFMKTHVEPTIRDIPSLLALAPWYGKKHRDNTLTMKRFTNGRGFWCLG
                     GKAAKNYREKSVDVAGYDELAAFDDDIEQEGSPTFLGDKRIEGSVWPKSIRGSTPKVR
                     GTCQIERAASESPHFMRFHVACPHCGEEQYLKFGDKETPFGLKWTPDDPSSVFYLCEH
                     NACVIRQQDLDFTDARYICEKTGIWTRDGILWFSSSGEEIEPPDSVTFHIWTAYSPFT
                     TWVQIVKDWMKTKGDTGKRKTFVNTTLGETWEAKIGERPDAEVMAERKEHYSAPVPDR
                     VAYLTAGIDSQLDRYEMRVWGWGPGEESWLIDRQIIMGRHDDEQTLLRVDEAINKTYI
                     RRNGAEMSVSRICWDTGGIDPTIVYERSKKHGLFRVIPIKGASVYGKPVASMPRKRNK
                     NGVYLTEIGTDTAKEQIYNRFTLTPEGDEPLPGAVHFPNNPDIFDLTEAQQLTAEEQV
                     EKWVDGRKKILWDSKKRRNEALDCFVYALAALRISISRWQLDLSALLASLQEEDGAAT
                     NKKTLADYARALSGEDE"
     gene            9224..9430
                     /locus_tag="J1Y83_002956"
     CDS             9224..9430
                     /locus_tag="J1Y83_002956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBA7631087.1"
                     /translation="MTRQEELAAARTALHDLMTGKRVATVQKDGRRVEFTATSVSDLK
                     KYIAELEVQTGMTQRRRGPAGFYV"
     gene            9427..11028
                     /locus_tag="J1Y83_002957"
     CDS             9427..11028
                     /locus_tag="J1Y83_002957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HBA7631088.1"
                     /translation="MKTPTIPTLLGPDGMTSLREYAGYHGGGSGFGGQLRAWNPPSES
                     VDAALLPNFTRGNARADDLVRNNGYAANAIQLHQDHIVGSFFRLSHRPSWRYLGIGEE
                     EARAFSREVEAAWKEFAEDDCCCIDVERKRTFTMMIREGVAMHAFNGELFVQATWDTS
                     SSRLFRTQFRMVSPKRISNPNNTGDSRNCRAGVQINDSGAALGYYVSEDGYPGWMPQK
                     WTWIPRVLPGGRASFIHVFEPVEDGQTRGANVFYSVMEQMKMLDTLQNTQLQSAIVKA
                     MYAATIESELDTQSAMDFILGANSQEQRERLTGWIGEIAAYYAAAPVRLGGAKVPHLM
                     PGDSLNLQTAQDTDNGYSVFEQSLLRYIAAGLGVSYEQLSRNYAQMSYSTARASANES
                     WAYFMGRRKFVASRQASQMFLCWLEEAIVRRVVTLPSKARFSFQEARSAWGNCDWIGS
                     GRMAIDGLKEVQEAVMLIEAGLSTYEKECAKRGDDYQEIFAQQVRETMERRAAGLKPP
                     AWAAAAFESGLRQSTEEEKSDSRAA"
     gene            11009..12340
                     /locus_tag="J1Y83_002958"
     CDS             11009..12340
                     /locus_tag="J1Y83_002958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000123221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HBA7631089.1"
                     /translation="MTAELRNLPHIASMAFNEPLMLEPAYARVFFCALAGQLGISRLT
                     DAVSGDSLTAPEAPATLALSGDDDGPRQARSYQVMNGIAVLPVSGTLVSRTRALQPYS
                     GMTGYNGIIARLQQAASDPMVDGILLDMDTPGGMVAGAFDCADIIARVRDIKPVWALA
                     NDMNCSAGQLLASAASRRLVTQTARTGSIGVMMAHSNYGAALEKQGVEITLIYSGSHK
                     VDGNPYSHLPDDVRETLQSRMDATRQMFAQKVSAYTGLSVQAVLDTEAAVYSGQEAID
                     AGLADELVNSTDAITVMRDALDARKSRLSGGRMTKETQSTTVSATASQADVTDVVPAT
                     EGENASAAQPDVNAQITAAVAAENSRIMGILNCEVAHGREEQARVLAETPGMTVETAR
                     RILAAAPQSAQARSDTALDRLMQGAPAPLAAGNLASDTNKELINTPEALPV"
     gene            12350..12682
                     /locus_tag="J1Y83_002959"
     CDS             12350..12682
                     /locus_tag="J1Y83_002959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head decoration protein"
                     /protein_id="HBA7631090.1"
                     /translation="MATKEEFNHYQPLGNSDPAHTAIAPGGLSAKTPAMTPLMLDGTT
                     RKLVVWDGTTDGAAVGILAVAADQTSATLTFYKSGSFRYEDVLWPEAASDETKKRTAF
                     AGTAISIV"
     gene            12738..13763
                     /locus_tag="J1Y83_002960"
     CDS             12738..13763
                     /locus_tag="J1Y83_002960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major capsid protein"
                     /protein_id="HBA7631091.1"
                     /translation="MSVYTTAELLASTQHHFKFDPLFLRLFFRETYPFTTEKVYLSQI
                     PGLVNMALYVSPIVSGEVIRSRGGSTSEFTPGYVKPKHEVNPQMTLRRLPDEDPQNLA
                     DPAYRRRRIIRQNMLDENLAIAQVEEMQAVSAVLKGKYTMTGEAFDPVEVDMGRSAAN
                     NITQSGGTEWSKRDKSTYDPTDDIEAYALNASGVVNIIVFDPKGWALFRSFKAVKEKL
                     DTRRGSHSELETAVKDLGKAVSYKGMYGDVAIVVYSGQYVENGVKKNFLPDNTMVLGN
                     TQARGLRTYGCIQDADAQREGINASARYPKNWVTTGDPAREFTMIQSAPLMLLADPDE
                     FVSVQLA"
     gene            13805..14200
                     /locus_tag="J1Y83_002961"
     CDS             13805..14200
                     /locus_tag="J1Y83_002961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein FI"
                     /protein_id="HBA7631092.1"
                     /translation="MTKDELIVRLRSLGEQLNRDVSLTGTKEELALRVAELEEELDDT
                     DDAAGQDTPLRPENALTGHENEVVSAQPDTMIDTAALVTVVALVTLHTDALHATRDEP
                     VAFVLPGTAFRVSAGMAAEMTERGLARMQ"
     gene            14212..14565
                     /locus_tag="J1Y83_002962"
     CDS             14212..14565
                     /locus_tag="J1Y83_002962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBA7631093.1"
                     /translation="MADFDNLFDAAIARADETIRGYMGTSATMTSGEQSGAVIRGVFD
                     DPENISYAGQGVRVEGSSPSLFVRTDDVRQLRRGDTLTIGEENFWIDRISTDDGGSCH
                     LWLGRGVPPAVNRRR"
     gene            14577..15155
                     /locus_tag="J1Y83_002963"
     CDS             14577..15155
                     /locus_tag="J1Y83_002963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBA7631094.1"
                     /translation="MAIKGLEQAVENLSRISKTVVPGASAMAINRVASSAISQSASQV
                     ARETRVRRKLVKERARLKRATVKNPQARIRVNRGDLPVIKLGNARVVLSRRRRRKKGQ
                     RSSLKGGCSVLVVGNRRIPGAFIQQLKNGRWHVMQRVAGKNRYPIDVVKIPMAVPLTT
                     AFKQNIERIRRERLPKELGYALQHQLRMVIKR"
     gene            15152..15547
                     /locus_tag="J1Y83_002964"
     CDS             15152..15547
                     /locus_tag="J1Y83_002964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBA7631095.1"
                     /translation="MKHTELRAAVLDALEKHDTGATFFDGRPAVFDEADFPAVAVYLT
                     GAEYTGEEMDSDTWQAELHIEVFLPAQVPDSELDSWMESRIYPVMSDIPALSDLITSM
                     VASGYDYRRDDDAGLWSSADLTYVITYEM"
     gene            15555..16295
                     /locus_tag="J1Y83_002965"
     CDS             15555..16295
                     /locus_tag="J1Y83_002965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBA7631096.1"
                     /translation="MPTPNPLVPVKGAGTTLWVYNGSGDPYANPLSDNDWSRLAKVKD
                     LTPGELTAESYDDSYLDDEDADWAATGQGQKSAGDTSFTLAWMPGEQGQQALLAWFNE
                     GDTRAYKIRFPNGTVDVFRGWVSSIGKAVTAKEVITRTVKVTNVGRPSMAEDRSTVTA
                     ATGMTVTPASSSVVKGQSTTLTVAFQPEGATDKSFRAVSADKTKATVSVSGMTITVKG
                     VAAGKVNIPVVSGNGELAAVAEINVTAS"
     gene            16311..16733
                     /locus_tag="J1Y83_002966"
     CDS             16311..16733
                     /locus_tag="J1Y83_002966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein G"
                     /protein_id="HBA7631097.1"
                     /translation="MFLKTESFEHNGVTVTLSELSALQRIEHLALMKRQAEQAESDSN
                     RKFTVEDAIRTGAFLVAMSLWHNHPQKTQMPSMNEAVKQIEQEVLTTWPTEAISHAEN
                     VVYRLSGMYEFVVNNAPEQTEDAGPAEPVSAGKCSTVS"
     gene            16715..17149
                     /locus_tag="J1Y83_002967"
     CDS             16715..17149
                     /locus_tag="J1Y83_002967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein T"
                     /protein_id="HBA7631098.1"
                     /translation="MFDGELSFALKLAREMGRPDWRAMLAGMSSTEYADWHRFYSTHY
                     FHDVLLDMHFSGLTYTVLSLFFSDPEMHPLDFSLLSRREADEEPEDDVLMQKAAGLAG
                     GVRFGPDGNEVIPASPDVADMTEDDVMLMTVSEGIAGGVRYG"
     gene            17142..>18366
                     /locus_tag="J1Y83_002968"
     CDS             17142..>18366
                     /locus_tag="J1Y83_002968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HBA7631099.1"
                     /translation="MAEPVGDLVVDLSLDAARFDEQMARVRRHFSGTESDAKKTAAVV
                     EQSMNRQALAAQKAGISVGQYKAAMRMLPAQFTDVATQLAGGQSPWLILLQQGGQVKD
                     SFGGMIPMFRGLAGAITLPMVGATSLAVATGALAYAWYQGNSTLSEFNKTLVLSGHQS
                     GLTADRMLVLSRAGQAAGLTFNQTSESLSALVKAGVSGEAQIASISQSVARFSSASGV
                     EVDKVAEAFGKLTTDPTSGLTAMARQFHNVTAEQIAYVAQLQRSGDEAGALQAANEAA
                     TKGFDDQTRRLKENMGTLETWADRTARAFKSMWDAVLDIGRPDTAQEMLIKAEAAFKK
                     ADDIWNLRKDDYFVNDEARARYWDDREKARLALEAARKKAEQQTQQDKNAQQQSDTEA
                     SRLKYTEEAQKAYERL"
BASE COUNT         4499 a         4433 c         5240 g         4194 t
ORIGIN      
        1 cggagcctga acaactccgg tgacttctgc gctaaacggg gacgtttatg cgcacataca
       61 atccaaactc tcttctccat tcacagatgc agaaatgcac ctgcgttttt ttgcatccag
      121 cgtctgacct ctgtggaggt gaagcgtgaa cctaccacaa gatggcatca aactgcatcg
      181 cggtaacttc accgctatcg gtcagcagat ccagccttat ctggaggacg gcaaatgctt
      241 tcgcatggtg cttaaaccgt ggcgtgagaa acgcagtctt tcccagaatg cactcagcca
      301 catgtggtac agcgaaatca gtgaatacct catcagcagg ggtaaaacgt tcgccactcc
      361 agcttgggta aaagatgctc tcaaacacac atatctcggt tatgaaacca aagacctggt
      421 tgatgtcgta accggtgata tcaccactat ccagtcgtta cgccatacct ccgatcttga
      481 taccggagag atgtatgtct tcctgtgtaa ggttgaagcc tgggcgatga atattggctg
      541 ccacctgact attccgcaga gctgcgagtt ccagcagctc cgcgacaaac aggaggcgta
      601 atggctacac cgcttattcg tgtcatgaac gggcacattt acagagtacc aaatcgtcgt
      661 aagcgtaaac ctgagctgaa gccttccgaa ataccaacac tgctcggata taccgccagc
      721 ctggttgata aaaaatggtt gcgactggca gcaaggagga atcatggctg atttgagaaa
      781 agcagcgcgt ggtcgggaat gccaggtaag aatccctggc gtatgtaatg gcaaccctga
      841 aacgtctgta ctggcacata tccggctggc tggattgtgc ggcaccggta ttaaaccgcc
      901 agaccttatt gccaccattg cctgttctgc ctgtcacgac gaaatcgacc gccgcacgca
      961 ttttgtcgat gctgagtacg caaaagaatg cgcgctggaa ggtatggcga gaacgcaggt
     1021 tatctggctg aaagaggggg taatcaaggc gtgaatactt accacatcac actaccctgg
     1081 ccgccgagca ataaccgcta ctacaggcat aatcgcgggc gcacgcacat cagcgcagaa
     1141 gggcaggcat accgtgataa cgtcgcccga atcattaaag gctcaatgct ggatatcggt
     1201 ctggctatgc ctgtgaaaat ccgcattgag tgccacatgc cggatcgccg tgacctggat
     1261 aatctgcaaa aagccgcttt tgacgcactc actaaagcag gtttctggct ggatgatgag
     1321 ctggtcgttg attaccgcgt tgtgaagatg cctgttacca aaggtgggaa gctggaactg
     1381 accatcactg aactggggaa tgaatgatgt ttgagtctta tatggcagag cgtcttcgcc
     1441 gccgctgggt gcgcctgcgc ttatatcgtt ttcctggttc tgttttgacc gattaccgga
     1501 tactgaagaa ttacgccaaa acactgaaag gagctgccgc atgaataccc aatatttaca
     1561 gtatgtccgc gagcaactca ttgtggctac cgctgatttg agcggagcaa cgaaaggaca
     1621 gcttgaagcc tggctggagc atgcacaatt tgatactggt acatacaaac gaaagaagct
     1681 gcgcattctg gatgaggtaa ctggcaggat gattacgctg gataatccgc cgatttccgg
     1741 taagcagtcg tacgcaaaag gttcatccat tgcactggta agccaggttg agttctcaac
     1801 ctcgtcatgg cgccgcgcgg ttctgtctct cgaagaacat cagaaagcgt ggttgctgtg
     1861 gagttacagc gaaagtgttc gctgggaaca tcaggtcacc ataacgcagt gggcatggag
     1921 cgagtttaag actctgttgg gtaccagaaa aattgcaggt aagacactgg aacgtttgaa
     1981 gaagttgatc tggctggcgg cacaggatgt gaagaacgag ctggcagggc gtaagaccta
     2041 tgaataccag gagctggcat cactggtggg agtgacatca aaaaactggt ctgagacatt
     2101 tactgaacgc tgggttgcaa tgaagcacat ttttctacag cttgatagcg aagctttatt
     2161 gcttgtaacg agaacacgtt caaaacaaaa gacgacattt tcacagcaaa gtattgcaaa
     2221 actggattga aaggcatata ttccgtgtaa atctgatatt ttgccaatgt tgtatgtgca
     2281 actgttcgta ggtgtcgtaa gggttgacta tccaatttcc aggatataga tttttctgca
     2341 ataaaggttt tgggaatgaa ggggcggctc ccaaaagata accgccaagt tggtaacttc
     2401 gactcgataa ttggtctggg actccaaccg tgtcggctga gaggtcgacg tctatttctt
     2461 ttggggaagt ggatacttta agctaacatt ttgagtttct atatctcgtg gccctcgagt
     2521 tttatcataa aaataccaga tattaaattt tctctcagtg tgccatgtat catattgcaa
     2581 atgatcccag cctaaatatg aagcaacata gtttgatact cttattttgg cttcagatga
     2641 agcttttcct tgatatgcgc acataaccgc acctaagaaa aaatcggcta gctgaatgtt
     2701 ttcagatgct ttagagtctt ttgtcactac actgctgatt atccctttac gaccaaattt
     2761 tctattcaat atgttattgg caataacatt tagttcttca tcggcctttt tataacgaga
     2821 tgcgatagga tcaacttcaa tcctgaagta actgtctctt tcagggtgtg cagaaattac
     2881 agagctaatt tttgtggtaa tgagatttgt gaagtgcttt ctccttgcca ggtcataatc
     2941 cccattatga aatgtcttgt ttactattga tttttcaacc acgatacaat gaaaagctag
     3001 ccaaggaact ctaaagaata agtcgatcag ctcaagataa aattcagagt atctttttga
     3061 atgggctttt tgccatttta tttcttcaaa aaaaccatgt ttgctacgta gttctctgat
     3121 tagttgacag aaatccccac ggcgttggta cttcatccac agactgccaa agccgtagaa
     3181 acgttgccca tcaatgccag attcatcgca agcaatatgc caaattaact taccaatgtt
     3241 atctttttct gtcatctgct ggtaatgctc ttagtcctta aagcatggta tttaaccaca
     3301 catgtaactc attgataaag atcgttttat gcaagttgca tggatcggct tttgaaggcc
     3361 attcgcttag ttataagatt ttttagttat tttgagttga ctgtttataa caatatttcc
     3421 catgtaagtt tttaatcatt agcgtcccgg cctttcgtcg gcggcgaaac attggctatt
     3481 catatgcacg aaaaagagag ccttgccgga gcgttctggc tcgttttgct gatcatcgca
     3541 ggttggggcg gtctggtccg ctatctgata gatgtgaagc aaagtaaagc aacgtggagc
     3601 tggataaatg ctctggctca gatagtggta tcgggattca ccggtgttat tggtggcctg
     3661 atcagtatcg aaagtggatt cagtatttac atgattctcg cgacagcggg gattagtggt
     3721 gcgatgggct cggttgcact gacgtacttc tgggaacgac tgacaggggt gaaaaatgca
     3781 aaatcttaat cctcagcgta aagctttcct cgatatgttg gcgtggtcag aaggaacgga
     3841 taacgggcga caaccgacac gtaaccgcgg ttatgacgtt attgtcggtg gcgaactctt
     3901 cactgattac tccgatcacc ctcgaaaact tgtcacgcta aacccgaaac tcaaatcaac
     3961 agccgcaggc cgttatcaac ttctttcacg ctggtgggat gcttaccgca agcagttagg
     4021 gctgacagac ttctctccta aaagccagga tgctgttgca ttgcagcaga ttaaggagcg
     4081 tggtgcttta ctaatgattg atcgcggtga catccgtcag gcaatcgact gttgcagcaa
     4141 tatctgggct tcgttacccg gagcaggtta cggtcagtat gaacataaaa tcggtgacct
     4201 gatttcccga tttaaagagg ctggtggggt ggtaaatgaa gttgagctat aagctggtta
     4261 tcgctgcttt tctcgttact gtcatcggtt ctttcatctg gtctgccaac cactactaca
     4321 gcaaatatca gcacgaaaag aaacgtgctg atgaggctgt acaaaatgcc aaatcggcaa
     4381 ctgtcattac caataacgtc ctgcaatcac tgcaaatcgt caatacagtt ctggaggcta
     4441 gccagcatgc aaaacagcag attgcactgg agtcacagag aacccagaca gatatcaaaa
     4501 cggctgttgc gaatgatgat tgcgctgccc gtcctgttcc tgctcgtgca gctgaacggc
     4561 tgcgccggta cgcggacagt ttacgtacca gttccagcgg taccgctacc ggcgaatctg
     4621 accactgaga ccccacaacc agttattcct gatccgctga cctatggggg cagtctggat
     4681 ttgaatgtca gtctgctttc cgcgctggcg acctgtaacc gagataaagc tgatattcga
     4741 aaaattgaag cagagaggat caagctatga agcagtaaag cggacggacc gtggcaagtg
     4801 gcgccaatgc agcagtcatg atgctgcccc gaatacccaa agttgcgggt agccagtctc
     4861 aggtaaaaag ctgagggcgt actggtgagg ggtaacggga agaagcgcac cggtactcca
     4921 ggctggtcgc cagtcagctt ccggttagtg gtgagggatt cacgagatac ccctgacaca
     4981 tggcctcaag ggaatgagcg cagctactgc gagagtgtgg ttggcattca cttagtgctt
     5041 gcgataatgc ttttagttac aatcacttca tcgataaatg aggtgaagat gaacgcagat
     5101 tatatagctt acgaatcatt ggttgccact agaaatacag cagaatgggc tttttggtca
     5161 atggtaggaa cgtggttttc aggcattgca acctttatgg cagtatgcct gactttatat
     5221 atttctaacc gtcgacctcg accgaagtta agaggaacag tatcgcttgg gggcatcaca
     5281 ggccaaaatg tgagcacgtt tggagttacg atcaacattg caaatgttgg cattcaaacc
     5341 gcaactataa cttcagtaac atggacgttc ggtacaaaaa actcactact tcagattctg
     5401 gggggtgggt ttggtgacga tttaccaaaa aaaattgagc atggcgaata tgcatttttc
     5461 tttattcaaa atgatactaa tggcgactgg gccagagata tgaaaaataa gatatgggaa
     5521 cagggcggca acgttcgcaa attaatgttg cttgtgcacc ttgctaccgg cgatacgatg
     5581 aaaattaagc cagccaaaaa tgtaataaaa atgatcgaaa aatcataaca tccacaggtg
     5641 tatcaccata gacagcctcc attctaatct caccatcaca ggatctcgta gagtgtctgt
     5701 gatggtgcta tatcctgtaa agtaaaggga gaccaatgat gacatcttta accgttgcgg
     5761 tattagcttt aatagttgca atctggcatg agataaaccg atttccagca accgggaaga
     5821 gcttactgtc tttacagcaa gaagttaggg agctgaggga tgaaaatgaa agattagcct
     5881 ctgaagtaga ttctttaaag gatgagttac ttgaaatttc aaatcagata gatcgaatta
     5941 aagaccctga atattatgca ttgcttgatg caggagatgg agtgggtctt tatgaaatag
     6001 aaaagtcaag aggtcaaatt taataaccgc ctttttggcg gtttattatt ccgtcatcat
     6061 gggcaggccc atcgtaactg cagtatctta tatacaccaa tgacatctct ggatggttgg
     6121 cagtcctgtt agttggcctg cggtggactc ggcctcgtca agcgtaagaa cacatacttt
     6181 ttggtatcgg ccagtagtaa agccggcgtg attttttggg ctgattaacg ggttgattga
     6241 tttgcatgag gctcgatgaa tccgcgtaaa tctggcatca ttttttctgc ctttcccgct
     6301 ggccgactta agcagattaa taacgaatcc atcggaatgg gttgctagcc aactcttgta
     6361 catcgcttcg ctatcaacat cctgtatttc actgctgaaa atagtaatgc ccatattttt
     6421 tccgtatatg actaactgat atgaatatgt atcaggacta ttttatggca aaaccagagt
     6481 agaaggccat tggggtccgg agtgttgatg tgaacaaatt aacccccccc tttaagtgta
     6541 attgatatgt attatcattt tcgggtcctt tccggcgatc cagaacgtta cggggcggaa
     6601 ggcgcgcggg ttttcgctat ttatgaaaat tttccggttt aaggcgtttc cgttcttctt
     6661 cgccgtaacc taatgttttt atttaaaacc ccccctgaaa agaaaggaaa cgacaggtgc
     6721 tgaaaacggg ctttttagcc tctgtcgttt cctttctctg tttttgtccg tggaatgaac
     6781 aatggaagtc aacaaaaagc agctggctga cattttcggt gcgagtatcc gtaccattca
     6841 gaactggcag gagcagggaa tgcccgttct gcgaggtggt gggaagggta atgaggtgct
     6901 ttatgactct gccgccgcca taaaatggta tgccgaaagg gatgctgaaa ttgagaacga
     6961 aaagctgcgc cgggaagttg aagaactgcg gcaggccagc gagacagatc tccagccagg
     7021 gactattgag tacgaacgcc atcgacttac gcgtgcgcag gccgacgcac aggagctgaa
     7081 aaatgccaga gactccgctg aagtggtgga aaccgcattc tgtactttcg tgctgtcgcg
     7141 gatcgcaggt gaaattgcca gtattctcga cgggatcccc ctgtcggtgc agcggcgttt
     7201 tccggaactg gaaaaccgac atgttgattt cctgaaacgg gatatcatca aagccatgaa
     7261 caaagcagcc gcgctggatg aactgatacc ggggttgctg agtgaatata tcgaacagtc
     7321 aggttaacag gctgcggcat tttgtccgcg ccgggcttcg ctcactgttc aggccggagc
     7381 cacagaccgc cgttgaatgg gcggatgcta attactatct cccgaaagaa tccgcatacc
     7441 aggaagggcg ctgggaaaca ctgccctttc agcgggccat catgaatgcg atgggcagcg
     7501 actacatccg cgaggtaaat gtggtgaagt ctgcccgtgt cggttattcc aaaatgctgt
     7561 tgggtgttta tgcctacttc atagagcata agcagcgcaa cacccttatc tggttgccga
     7621 cggatggtga tgccgagaac tttatgaaaa cccacgtcga gccgaccatc cgtgatattc
     7681 cgtcgctgct ggcgctggcc ccgtggtatg gcaaaaagca ccgggataac acgctcacca
     7741 tgaagcgttt caccaatggt cgtggcttct ggtgcctggg cggtaaagcg gcaaaaaact
     7801 accgtgaaaa gtcagtggat gtggcgggtt atgatgaact tgctgccttt gatgatgata
     7861 ttgaacagga aggctctccg acgttcctgg gtgacaagcg tattgaaggc tcggtctggc
     7921 caaagtccat ccgtggctcc acgcccaaag tgagaggcac ctgtcagatt gagcgtgcag
     7981 ccagtgaatc cccgcatttt atgcgttttc atgttgcctg cccgcactgc ggggaggagc
     8041 agtaccttaa atttggcgat aaagagacgc cgtttggcct caaatggacg ccggatgatc
     8101 cctccagcgt gttttatctc tgcgaacata atgcctgcgt catccgccag caggatctgg
     8161 acttcactga tgcccgttat atctgcgaaa agaccgggat ctggacccgt gatggcattc
     8221 tctggttttc gtcatccggt gaagagattg agccgccgga cagcgtgacc tttcacatct
     8281 ggacggcgta cagcccgttc accacctggg tgcagattgt caaagactgg atgaaaacga
     8341 aaggggatac gggaaaacgt aaaaccttcg tgaacaccac gctcggtgag acatgggaag
     8401 cgaaaatcgg tgaacgtccg gatgctgaag tgatggcaga gcggaaagag cattattcag
     8461 cgcccgttcc tgaccgtgtg gcttacctga ctgccggtat cgactcccag ctggatcgct
     8521 acgaaatgcg cgtatgggga tgggggccgg gtgaggaaag ctggctaatt gaccggcaga
     8581 ttattatggg ccgccacgat gatgaacaga cgctgctgcg tgtggatgag gccatcaata
     8641 aaacctatat ccgccggaat ggtgcagaaa tgtcggtatc ccgtatctgc tgggatactg
     8701 gcgggattga cccgaccatt gtgtatgaac gctcgaaaaa acatgggctg ttccgggtga
     8761 tccccattaa aggggcatcc gtctacggaa agccggtggc cagcatgcca cgtaagcgaa
     8821 acaaaaacgg ggtttacctt accgaaatcg gtacggatac cgcgaaagag cagatttata
     8881 accgcttcac actgacgccg gaaggggatg aaccgcttcc cggtgccgtt cacttcccga
     8941 ataacccgga tatttttgat ctgaccgaag cgcagcaact gactgctgaa gagcaggtcg
     9001 aaaaatgggt ggatggcagg aaaaaaatac tgtgggacag caaaaagcga cgcaatgagg
     9061 cactcgactg cttcgtttat gcgctggcgg cgctgcgcat cagtatttcc cgctggcagc
     9121 tggatctcag tgcactgctg gcgagcctgc aggaagagga tggtgcagca accaacaaga
     9181 aaacactggc agattacgcc cgtgccttat ccggagagga tgaatgacgc gacaggaaga
     9241 acttgccgct gcccgtacgg cactgcatga cctgatgaca ggtaaacggg tggcaacagt
     9301 acagaaagac ggacggcgag tggagtttac ggccacttcc gtgtctgacc tgaaaaaata
     9361 cattgcggag ctggaagtgc agaccggcat gacacagcga cgcaggggac ctgcaggatt
     9421 ttatgtatga aaacgcccac cattcccacc cttctggggc cggacggcat gacatcgctg
     9481 cgtgaatatg ccggttatca tggcggtggc agcggatttg gtgggcagtt gcgggcgtgg
     9541 aatccaccga gtgaaagtgt ggatgcagcc ctgctgccca actttacccg tggcaatgcc
     9601 cgcgcagacg atctggtacg caataacggc tatgctgcca acgccatcca gctgcatcag
     9661 gatcatatcg tcgggtcttt tttccggctc agtcatcgcc caagctggcg ctatctgggc
     9721 atcggggagg aagaagcccg tgccttttcc cgcgaggttg aagcggcatg gaaagagttt
     9781 gccgaggatg actgctgctg cattgacgtt gagcgaaaac gcacgtttac catgatgatt
     9841 cgggaaggtg tggccatgca cgcctttaac ggtgaactgt tcgttcaggc cacctgggat
     9901 accagttcgt cgcggctttt ccggacacag ttccggatgg tcagcccgaa gcgcatcagc
     9961 aacccgaaca ataccggcga tagccggaac tgccgtgccg gtgtgcagat taatgacagc
    10021 ggtgctgcgc tgggatatta cgtcagcgag gacgggtatc ctggctggat gccgcagaaa
    10081 tggacatgga taccccgtgt gttacccggc gggcgcgcct cgttcattca cgtttttgaa
    10141 cccgtggagg acgggcagac tcgcggtgca aatgtgtttt acagcgtgat ggagcagatg
    10201 aagatgctcg acacgctgca gaacacgcag ctgcagagcg ccattgtgaa ggcgatgtat
    10261 gccgccacca ttgagagtga gctggatacg cagtcagcga tggattttat tctgggcgcg
    10321 aacagtcagg agcagcgaga aaggctgacg ggctggattg gtgaaattgc cgcgtattac
    10381 gccgcagcgc cggtccggct gggaggcgca aaagtgccac acctgatgcc aggtgactca
    10441 ctgaacctgc agacggctca ggacacggat aacggctact ccgtgtttga gcagtcactg
    10501 ctgcggtata tcgctgccgg gctgggtgtc tcgtatgagc agctttcccg gaattacgcc
    10561 cagatgagct actccacggc acgggccagt gcgaacgagt cgtgggcgta ctttatgggg
    10621 cggcgaaaat tcgtcgcatc ccgtcaggcg agccagatgt ttctgtgctg gctggaagag
    10681 gccatcgttc gccgcgtggt gacgttacct tcaaaagcgc gcttcagttt tcaggaagcc
    10741 cgaagtgcct gggggaactg tgactggata ggctccggtc gtatggccat cgatggtctg
    10801 aaagaagtac aggaagcggt gatgctgata gaagccgggc tgagtaccta cgagaaagag
    10861 tgtgcaaaac gcggcgatga ctatcaggaa atttttgccc agcaggtccg tgaaacgatg
    10921 gagcgccgcg cggctggtct taaaccgccc gcctgggcgg ctgcggcatt tgaatccgga
    10981 ctgcgacaat caacagagga ggagaagagt gacagcagag ctgcgtaatc tcccgcatat
    11041 tgccagcatg gcttttaatg agccgctgat gcttgaaccc gcctatgcgc gggttttctt
    11101 ttgtgcgctt gcaggccagc ttgggatcag ccgcctgacg gatgcagtat ccggcgacag
    11161 cctgactgcc ccggaggcac ccgcgacgct ggcgttatcc ggtgatgatg acggaccacg
    11221 acaggcccgc agttatcagg tcatgaacgg gatcgccgtg ctgccggttt ccggcacgct
    11281 ggtcagccgg acgcgggcgc tgcagccgta ttcggggatg accggttaca acggcattat
    11341 cgcccgtctg caacaggctg ccagcgatcc gatggtggac ggcattctgc tcgatatgga
    11401 cacgccaggc ggaatggtgg cgggagcatt tgactgcgct gacatcatcg cccgtgtgcg
    11461 tgacataaaa ccggtatggg cgctggccaa cgacatgaac tgcagtgcag gtcagctgct
    11521 tgccagcgcc gcttcccggc gtctggtcac gcagaccgcc cggacaggct ccatcggcgt
    11581 catgatggct cacagtaatt acggtgctgc gctggagaaa cagggtgtgg aaatcacgct
    11641 gatttacagc ggcagccata aggtggatgg caacccctac agccatcttc cggatgacgt
    11701 ccgggagaca ctgcagtccc ggatggatgc aacccgtcag atgtttgcgc agaaggtgtc
    11761 ggcatatacc ggtctgtccg tgcaggccgt gctggatacc gaggctgcag tgtacagcgg
    11821 tcaggaggcc attgatgccg gactggctga tgaacttgtt aacagcaccg atgcgatcac
    11881 cgttatgcgt gatgcactgg atgcacgtaa atcccgactc tcaggagggc gaatgaccaa
    11941 agagactcaa tcaacaactg tttcagccac tgcttcgcag gctgacgtta ctgacgtggt
    12001 gccagcgacg gagggcgaaa acgccagcgc ggcgcaaccg gacgtgaacg cgcagatcac
    12061 cgcagcggtt gcggcagaaa acagccgcat tatggggatc ctcaactgtg aggtggctca
    12121 cggacgcgaa gaacaggcac gcgtgctggc agaaaccccc ggtatgaccg tggaaacggc
    12181 ccgccgcatt ctggccgcag caccacagag tgcacaggcg cgcagtgata ctgcgctgga
    12241 tcgtctgatg cagggtgcac cggcaccgct ggctgcaggt aacctggcat ctgataccaa
    12301 taaagaatta attaatacac ctgaagcttt accggtataa gaggcagtta tggcgacaaa
    12361 agaagagttt aaccattacc agccgctggg taacagtgat ccggctcata cagcaattgc
    12421 gcctggcgga ttgagtgcga aaacgcctgc aatgaccccg ctgatgctgg atggcactac
    12481 ccgtaagctg gttgtgtggg atggcacaac cgacggtgca gccgttggca ttctggcggt
    12541 tgctgctgac cagaccagcg ccacactgac gttctacaag tccggctcgt tccgttatga
    12601 ggatgtgctc tggccggagg ctgccagcga cgagacgaaa aaacggaccg cgtttgccgg
    12661 aacggcaatc agcatcgttt aatcttcccc ttcatcaaca aaggccgcct gtgcggcttt
    12721 ttttatggaa ataatttatg tctgtatata caactgcaga attactggca tcgacccagc
    12781 atcactttaa gttcgatccg ctgtttctgc gcctgttttt ccgtgaaacc tatcctttca
    12841 ccacggaaaa agtctatctc tcgcaaattc cgggactggt aaacatggcg ctgtacgttt
    12901 cgccgattgt ttccggtgag gttatccgct cccgtggcgg ctccacatct gaatttacgc
    12961 cgggttatgt caagccgaag catgaggtga atccgcagat gaccctgcgt cgcctgccgg
    13021 atgaagatcc gcagaacctg gctgacccgg cttaccgtcg tcgccgtatt attcggcaga
    13081 atatgctgga tgaaaatctg gcgattgccc aggtcgaaga gatgcaggca gtttctgccg
    13141 tgcttaaggg caaatacacc atgaccggtg aagcctttga tccggttgag gtggatatgg
    13201 gccgcagtgc ggcgaacaac atcacacagt ccggtggcac ggagtggagc aagcgtgaca
    13261 agtccacgta tgacccgacc gacgatatcg aagcctacgc gctgaacgcc agcggtgtgg
    13321 tgaatatcat cgtgtttgat ccgaaaggct gggcgctgtt ccgttccttc aaagccgtca
    13381 aggagaagct ggatacccgt cgtggctctc attccgagct ggagacagcg gtgaaagacc
    13441 tgggtaaagc ggtgtcctat aaggggatgt atggcgatgt ggccatcgtc gtgtattccg
    13501 gacagtacgt ggaaaacggc gtcaaaaaga acttcctgcc ggacaacacg atggtgctgg
    13561 ggaacactca ggcacgcggt ctgcgcacct atggctgcat tcaggatgcg gacgcacagc
    13621 gcgaaggcat taacgcctct gcccgttacc cgaaaaactg ggtgaccacc ggcgatccgg
    13681 cgcgtgagtt caccatgatt cagtcagcac cgctgatgct gctggctgac cctgatgagt
    13741 tcgtgtccgt acaactggcg taatcgtggc ccttcggggc cattttctct ctgtggagga
    13801 gtccatgacg aaagatgaac tgattgtccg tctccggtcg ctgggtgagc aactgaaccg
    13861 tgatgtcagc ctgacgggga cgaaagaaga actggcgctc cgtgtggcag agctggaaga
    13921 agagcttgat gacacggatg acgctgccgg tcaggatacc cctctcagac cggaaaatgc
    13981 gctgaccgga catgaaaatg aggtggtatc agcgcagccg gataccatga ttgatacggc
    14041 tgctctggtc acggtcgtgg cactggtgac gctgcatact gatgcacttc acgccacgcg
    14101 ggatgaacct gtggcatttg tgctgccggg aacggcgttt cgtgtctctg ccggtatggc
    14161 agccgaaatg acagaacgtg gcctggccag aatgcaataa cgggaggcgc tgtggctgat
    14221 ttcgataacc tgttcgatgc tgccattgcc cgcgccgatg aaacgatacg cgggtacatg
    14281 ggaacgtcag ccaccatgac atccggtgag cagtccggcg cagtaatacg tggtgttttt
    14341 gatgaccctg aaaatatcag ctatgccgga cagggcgtgc gcgttgaagg ctccagcccg
    14401 tccctgtttg tccggactga tgatgtgcgg cagctgcggc gcggcgacac gctgaccatc
    14461 ggtgaggaaa acttctggat agaccggatt tcgacggatg atggcggaag ctgtcatctc
    14521 tggcttgggc gtggcgtgcc gcctgccgtt aaccgtcgtc gctgaagggg ggatgtatgg
    14581 ccataaaagg tcttgagcag gccgttgaaa acctcagtcg tatcagcaaa acggtggtgc
    14641 ccggtgcgtc agcaatggcc attaaccgcg ttgcttcatc cgcgatatcg cagtctgcgt
    14701 cacaggttgc ccgtgagaca agggtacgcc ggaaactggt aaaggaaagg gccaggctga
    14761 aaagggccac ggtcaaaaat ccgcaggcca gaatcagggt taaccggggg gatttgcccg
    14821 taatcaagct gggtaacgcg cgggttgtcc tgtcccgacg caggcgtcgt aaaaaggggc
    14881 agcgttcatc cctgaaaggt ggctgcagcg tgcttgtggt gggaaaccgt cgtattcccg
    14941 gcgcgtttat tcagcaactg aaaaatggcc ggtggcatgt catgcagcgt gtggccggga
    15001 aaaaccgtta ccccattgat gtggtgaaaa tcccgatggc ggtgccgctg accacggcgt
    15061 ttaaacagaa tattgagcgg atacggcgtg aacgtcttcc gaaagagctg ggctatgcgc
    15121 tgcagcatca actgagaatg gtaataaagc gatgaaacat actgaactcc gtgcagccgt
    15181 actggatgca ctggagaagc atgacaccgg ggcgacgttt tttgatggtc gccccgctgt
    15241 ttttgatgag gcggattttc cggcagttgc cgtttatctc accggcgctg aatacacggg
    15301 cgaagagatg gacagcgata cctggcaggc ggagctgcat atcgaagttt tcctgcctgc
    15361 tcaggtgccg gattcagagc tggattcgtg gatggagtcc cggatttatc cggtgatgag
    15421 cgatattccg gcactgtcag atttgatcac cagtatggtg gccagtggct atgactaccg
    15481 gcgcgacgat gatgcgggcc tgtggagttc agccgatctg acttatgtca ttacctatga
    15541 aatgtgagga cgatatgcca acaccaaatc ctctggtacc ggtgaaaggg gccgggacca
    15601 cactgtgggt ttataacggg agcggcgacc cttacgcaaa cccgctttca gacaatgact
    15661 ggtcgcgtct ggcaaaggtt aaagacctga cgcccggcga actgaccgct gagtcctatg
    15721 acgacagtta tctcgatgat gaagatgcgg actgggccgc gaccggacag gggcagaaat
    15781 ccgctggaga taccagtttc acgctggcgt ggatgcccgg agagcagggg cagcaggcgc
    15841 tgctggcgtg gtttaatgaa ggcgataccc gtgcctataa aatccgcttc ccgaacggca
    15901 cggtcgatgt gttccgtggc tgggtcagca gtatcggtaa ggcggtgacg gcgaaggaag
    15961 tgatcacccg cacggtgaaa gtcaccaatg tgggacgtcc gtcgatggca gaagatcgca
    16021 gcacggtaac agcggcaacc ggtatgaccg tgacgcctgc cagctcctcg gtggtgaaag
    16081 ggcagagcac cacgctgacc gtggcattcc agccggaggg cgcaactgac aagagcttcc
    16141 gtgcggtgtc agcggataaa acaaaagcca ccgtgtcggt cagtggtatg accatcaccg
    16201 tgaaaggcgt tgctgcaggt aaggtcaaca ttccggtcgt atccggtaat ggtgaacttg
    16261 ctgcggttgc agaaatcaac gtcaccgcca gttaatccgg agagtcagcg atgttcctga
    16321 aaaccgaatc atttgaacat aacggtgtga ccgtcacgct ttctgaactg tcagccctgc
    16381 agcgcattga gcatctcgcc ctgatgaaac ggcaggcaga acaggcggag tcagacagca
    16441 accggaagtt tactgtggaa gacgccatca gaaccggcgc gtttctggtg gcgatgtccc
    16501 tgtggcataa ccatccgcag aagacgcaga tgccgtccat gaatgaagcc gttaaacaga
    16561 ttgagcagga agtgcttacc acctggccca cggaggcaat ttctcatgct gaaaacgtgg
    16621 tgtaccggct gtctggtatg tatgagtttg ttgtgaataa tgcccctgaa cagacagagg
    16681 acgccgggcc tgcagagcct gtttctgcgg gaaagtgttc gacggtgagc tgagttttgc
    16741 cctgaaactg gcgcgtgaga tggggcgacc cgactggcgt gccatgcttg ccgggatgtc
    16801 atccacggag tatgccgact ggcaccgctt ttacagtacc cattattttc atgatgttct
    16861 gctggatatg cacttttccg ggctgacgta caccgtgctc agcctgtttt tcagcgatcc
    16921 ggagatgcat ccgctggatt tcagtctgct gagccggcgt gaggctgacg aagagcctga
    16981 agatgatgtg ctgatgcaga aagcggcagg gcttgccgga ggtgtccgct ttggcccgga
    17041 cgggaatgaa gttatccccg cttccccgga tgtggcggac atgacggagg atgacgtaat
    17101 gctgatgaca gtatcagaag ggatcgcagg aggagtccgg tatggctgaa ccggtaggcg
    17161 atctggtcgt tgatttgagt ctggatgcgg ccagatttga cgagcagatg gccagagtca
    17221 ggcgtcattt ttccggtacg gaaagtgatg cgaaaaaaac agcggcagtc gttgaacagt
    17281 cgatgaaccg acaggcgctg gctgcacaga aagcggggat ttccgtcggg cagtataaag
    17341 ctgccatgcg tatgctgcct gcgcagttca ccgacgtggc cacgcagctt gcaggcgggc
    17401 aaagtccgtg gctgatcctg ctgcaacagg gtggtcaggt gaaggactcc ttcggcggga
    17461 tgatccccat gttcaggggg cttgccggtg cgatcaccct gccgatggtc ggggccacct
    17521 cgctggcagt ggcgaccggt gcgctggcgt atgcctggta tcagggcaac tcaaccctgt
    17581 ctgagttcaa caaaacgctg gtcctttccg gtcatcagtc aggtctgacg gcagatcgca
    17641 tgctggtcct gtccagagcc gggcaggcgg cagggctgac gtttaaccag accagcgagt
    17701 cactcagcgc actggttaag gcgggggtaa gcggtgaggc tcagattgcg tccatcagcc
    17761 agagtgtggc gcgtttctcc tctgcatccg gcgtggaggt ggacaaggtc gctgaagcct
    17821 tcgggaagct gaccacagac ccgacgtcgg ggctgacggc gatggcgcgc cagttccata
    17881 acgtgacggc ggagcagatt gcgtatgttg ctcagttgca gcgttccggc gatgaggccg
    17941 gggcattgca ggcggcgaac gaggccgcaa cgaaagggtt tgatgaccag acccgccgcc
    18001 tgaaagagaa catgggcacg ctggagacct gggcagacag gactgcacgg gcattcaaat
    18061 ccatgtggga tgcggtgctg gatattggtc gtcctgatac cgcgcaggag atgctgatta
    18121 aggcagaggc tgcgtttaag aaagcagacg acatctggaa tctgcgcaag gatgattatt
    18181 ttgttaacga tgaagcgcgg gcgcgttact gggatgatcg tgaaaaggcc cgtcttgcgc
    18241 ttgaagccgc ccgaaagaag gctgagcagc agactcaaca ggacaaaaat gcgcagcagc
    18301 agagcgatac cgaagcgtca cggctgaaat ataccgaaga ggcgcagaag gcttacgaac
    18361 ggctgc
//
LOCUS       DADSYM010000101        18239 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2415
            SAMN08039579-rid11723173.denovo.101, whole genome shotgun sequence.
ACCESSION   DADSYM010000101 DADSYM010000000
VERSION     DADSYM010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039579
            Sequence Read Archive: SRR6362533
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18239)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18239)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980162.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 03:39:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,484
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 4,347
            CDSs (with protein)               :: 4,347
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 43 of 85
            Pseudo Genes (incomplete)         :: 37 of 85
            Pseudo Genes (internal stop)      :: 19 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18239
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039579-rid11723173.denovo.101"
                     /strain="LGE2415"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8302 N 95.9628 W"
                     /collection_date="2015-08-24"
     gene            complement(113..859)
                     /gene="cutC"
                     /locus_tag="J1Y83_002969"
     CDS             complement(113..859)
                     /gene="cutC"
                     /locus_tag="J1Y83_002969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HBA7631100.1"
                     /translation="MALLEICCYSMECALTAQQNGADRVELCAAPKEGGLTPSLGVLK
                     SVRQRVTIPVHPIIRPRGGDFCYSDGEFAAILEDVRTVRELGFPGLVTGVLDVDGNVD
                     MPRMEKIMAAAGPLAVTFHRAFDMCANPLNTLNNLAELGITRVLTSGQKSDALQGLSK
                     IMELIAHRDAPIIMAGAGVRAENLHHFLDAGVLEVHSSAGAWQASPMRYRNQGLSMSS
                     DAHADEYSRYVVDGAAVAEMKGIIERHQAK"
     gene            complement(873..1439)
                     /locus_tag="J1Y83_002970"
     CDS             complement(873..1439)
                     /locus_tag="J1Y83_002970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HBA7631101.1"
                     /translation="MANWQSIDELQDIASDLPRFTHALDELSRRLGLDITPLTADHIS
                     LRCHQNVTAERWRRGFEQCGELLSENMINGRPICLFKLHEPVQVAHWQFSIVELPWPG
                     EKRYPHEGWEHIEIVLPGDPETLNARALALLSDEGLSLPGISVKTSSPKGEHERLPNP
                     TLAVTDGKTTIKFHPWSIEEIVASEQSA"
     gene            1655..3388
                     /gene="argS"
                     /locus_tag="J1Y83_002971"
     CDS             1655..3388
                     /gene="argS"
                     /locus_tag="J1Y83_002971"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001025321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HBA7631102.1"
                     /translation="MNIQALLSEKVRQAMIAAGAPADCEPQVRQSAKVQFGDYQANGM
                     MAVAKKLGMAPRQLAEQVLTHLDLNGIASKVEIAGPGFINIFLDPAFLAEHVQQALAS
                     DRLGVAMPEKQTIVVDYSAPNVAKEMHVGHLRSTIIGDAAVRTLEFLGHKVIRANHVG
                     DWGTQFGMLIAWLEKQQQENAGEMELADLEGFYRDAKKHYDEDEEFAERARNYVVKLQ
                     SGDEYFREMWRKLVDITMTQNQITYDRLNVTLTRDDVMGESLYNPMLPGIVADLKAKG
                     LAVESEGATVVFLDEFKNKEGEPMGVIIQKKDGGYLYTTTDIACAKYRYETLHADRVL
                     YYIDSRQHQHLMQAWAIVRKAGYVPESVPLEHHMFGMMLGKDGKPFKTRAGGTVKLAD
                     LLDEALERARRLVAEKNPDMPADELEKLANAVGIGAVKYADLSKNRTTDYIFDWDNML
                     AFEGNTAPYMQYAYTRVLSVFRKAEINEEQLAAAPVIIREDREAQLAARLLQFEETLT
                     VVAREGTPHVMCAYLYDLAGLFSGFYEHCPILSAENEEVRNSRLKLAQLTAKTLKLGL
                     DTLGIETVERM"
     gene            complement(3441..3833)
                     /locus_tag="J1Y83_002972"
     CDS             complement(3441..3833)
                     /locus_tag="J1Y83_002972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FlhE"
                     /protein_id="HBA7631103.1"
                     /translation="MRALLAILLFPLLVQAAGEGMWQASSVGVTLNHRGESMSSAPLS
                     TRQPASGLMTLVAWHYQLIGPTPAGLRVRLCSQSRCVELEGQSGTTVAFSGIPAAEPL
                     RFIWEVPGGGRLIPPLKIQRNEVIVNYR"
     gene            complement(3833..5911)
                     /gene="flhA"
                     /locus_tag="J1Y83_002973"
     CDS             complement(3833..5911)
                     /gene="flhA"
                     /locus_tag="J1Y83_002973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
                     /protein_id="HBA7631104.1"
                     /translation="MSNLAAMLRLPANLKSTQWQILAGPILILLILSMMVLPLPAFIL
                     DLLFTFNIALSIMVLLVAMFTQRTLEFAAFPTILLFTTLLRLALNVASTRIILMEGHT
                     GAAAAGKVVEAFGHFLVGGNFAIGIVVFVILVIINFMVITKGAGRIAEVGARFVLDGM
                     PGKQMAIDADLNAGLIGEDEAKKRRSEVTQEADFYGSMDGASKFVRGDAIAGILIMVI
                     NVVGGLLVGVLQHGMSMGHAAESYTLLTIGDGLVAQIPALVISTAAGVIVTRVSTDQD
                     VGEQMVNQLFSNPSVMLLSAAVLGLLGLVPGMPNLVFLLFTAGLLGLAWWIRGREQKS
                     PAEPKPVKMAENNTVVEATWNDVQLEDSLGMEVGYRLIPMVDFQQDGELLGRIRSIRK
                     KFAQEMGFLPPVVHIRDNMDLQPAGYRILMKGVEIGSGDAYPGRWLAINPGTAAGTLP
                     GEATVDPAFGLNAIWIESALKEQAQIQGYTVVEASTVVATHLNHLISQHAAELFGRQE
                     AQQLLDRVAQEMPKLTEDLVPGVVTLTTLHKVLQNLLDEKVPIRDMRTILETLAEHAP
                     IQSDPHELTAVVRVALGRAITQQWFPGKDEVHVIGLDTPLERLLLQALQGGGGLEPGL
                     ADRLLAQTQEALSRQEMLGAPPVLLVNHALRPLLSRFLRRSLPQLVVLSNLELSDNRH
                     IRMTATIGGK"
     gene            complement(5904..7052)
                     /gene="flhB"
                     /locus_tag="J1Y83_002974"
     CDS             complement(5904..7052)
                     /gene="flhB"
                     /locus_tag="J1Y83_002974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system protein
                     FlhB"
                     /protein_id="HBA7631105.1"
                     /translation="MSDESDDKTEAPTPHRLEKAREEGQIPRSRELTSLLILLVGVSV
                     IWFGGVSLARRLSGMLSAGLHFDHSIINDPNLILGQIILLIREAMLALLPLISGVVLV
                     ALISPVMLGGLVFSGKSLQPKFSKLNPLPGIKRMFSAQTGAELLKAILKTILVGSVTG
                     FFLWHHWPQMMRLMAESPITAMGNAMDLVGLCALLVVLGVIPMVGFDVFFQIFSHLKK
                     LRMSRQDIRDEFKQSEGDPHVKGRIRQMQRAAARRRMMADVPKADVIVNNPTHYSVAL
                     QYDENKMSAPKVVAKGAGLVALRIREIGAENNVPTLEAPPLARALYRHAEIGQQIPGQ
                     LYAAVAEVLAWVWQLKRWRLAGGQRPVQPTHLPVPEALDFINEKPTHE"
     gene            complement(7261..7905)
                     /gene="cheZ"
                     /locus_tag="J1Y83_002975"
     CDS             complement(7261..7905)
                     /gene="cheZ"
                     /locus_tag="J1Y83_002975"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase CheZ"
                     /protein_id="HBA7631106.1"
                     /translation="MMQPSIKPADEHSAGDIIARIGSLTRMLRDSLRELGLDQAIAEA
                     AEAIPDARDRLYYVVQMTAQAAERALNSVEASQPHQDQMEKSAKALTQRWDDWFADPI
                     DLADARELVTDTRQFLADVPAHTNFTNAQLLEIMMAQDFQDLTGQVIKRMMDVIQEIE
                     RQLLMVLLENIPEQESRPKRENQSLLNGPQVDTSKAGVVASQDQVDDLLDSLGF"
     gene            complement(7916..8305)
                     /gene="cheY"
                     /locus_tag="J1Y83_002976"
     CDS             complement(7916..8305)
                     /gene="cheY"
                     /locus_tag="J1Y83_002976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheY"
                     /protein_id="HBA7631107.1"
                     /translation="MADKELKFLVVDDFSTMRRIVRNLLKELGFNNVEEAEDGLDALN
                     KLQAGGYGFVISDWNMPNMDGLELLKTIRADGAMSALPVLMVTAEAKKENIIAAAQAG
                     ASGYVVKPFTAATLEEKLNKIFEKLGM"
     gene            complement(8320..9369)
                     /locus_tag="J1Y83_002977"
     CDS             complement(8320..9369)
                     /locus_tag="J1Y83_002977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000036372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis response regulator protein-glutamate
                     methylesterase"
                     /protein_id="HBA7631108.1"
                     /translation="MSKIRVLSVDDSALMRQIMTEIINSHSDMEMVATAPDPLVARDL
                     IKKFNPDVLTLDVEMPRMDGLDFLEKLMRLRPMPVVMVSSLTGKGSEVTLRALELGAI
                     DFVTKPQLGIREGMLAYSEMIAEKVRTAAKASLAAHKPLSVPTTLKAGPLLSSEKLIA
                     IGASTGGTEAIRHVLQPLPLSSPALLITQHMPPGFTRSFADRLNKLCQIGVKEAEDGE
                     RVLPGHAYIAPGDRHMELARSGANYQIKIHDGPAVNRHRPSVDVLFHSVAKQAGRNAV
                     GVILTGMGNDGAAGMLAMRQAGAWTLAQNEASCVVFGMPREAINMGGVCEVVDLSQVS
                     QQMLAKISAGQAIRI"
     gene            complement(9372..10232)
                     /gene="cheR"
                     /locus_tag="J1Y83_002978"
     CDS             complement(9372..10232)
                     /gene="cheR"
                     /locus_tag="J1Y83_002978"
                     /EC_number="2.1.1.80"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate O-methyltransferase CheR"
                     /protein_id="HBA7631109.1"
                     /translation="MTSSLPCGQMSLLLQMTERLALSDAHFRRISQLIYQRAGIVLAD
                     HKRDMVYNRLVRRLRALGLADFGHYLNLLESNQHSGEWQAFINSLTTNLTAFFREAHH
                     FPLLADHARRRSGEYRVWSAAASTGEEPYSIAMTLANTLGTAPGRWKVFASDIDTEVL
                     EKARSGIYRHEELKNLTPQQLQRYFMRGTGPHEGLVRVRQELANYVDFAPLNLLAKQY
                     TVPGPFDAIFCRNVMIYFDQTTQQEILRRFVSLLKPDGLLFAGHSENFSHLERRFTLR
                     GQTVYALSKD"
     gene            complement(10251..10477)
                     /locus_tag="J1Y83_002979"
                     /pseudo
     CDS             complement(10251..10477)
                     /locus_tag="J1Y83_002979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310622.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(10523..12184)
                     /gene="tar"
                     /locus_tag="J1Y83_002980"
     CDS             complement(10523..12184)
                     /gene="tar"
                     /locus_tag="J1Y83_002980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein II"
                     /protein_id="HBA7631110.1"
                     /translation="MINRIRVVTLLVMVLGVFALLQLISGSLFFSSLHHSQKSFVVSN
                     QLREQQGELTSTWDLMLQTRINLSRSAVRMMMDSSNKQSNAKVELLDSARKTLAQAAT
                     HYKKFKSMAPLPEMVATSRNIDEKYKNYHTALTELIDYLDYGNTGAYFAQPTQGMQNA
                     MGEAFAQYALSSEKLYRDIVTDNADDYRFAQWQLAVIALVVVLILLVAWYGIRRMLLT
                     PLAKIIAHIREIAGGNLANTLTIDGRSEMGDLAQSVSHMQRSLTDTVTHVRQGSDAIY
                     AGTREIAAGNTDLSSRTEQQASALEETAASMEQLTATVKQNADNARQASQLAQSASDT
                     AQHGGKVVDGVVKTMHEIADSSKKIADIISVIDGIAFQTNILALNAAVEAARAGEQGR
                     GFAVVAGEVRNLASRSAQAAKEIKALIEDSVSRVDTGSVLVESAGETMNNIVNAVTRV
                     TDIMGEIASASDEQSRGIDQVALAVSEMDRVTQQNASLVQESAAAAAALEEQASRLTQ
                     AVSAFRLAASPLTNKPQTPSRPASEQPPAQPRLRIAEQDPNWETF"
     gene            complement(12329..12832)
                     /gene="cheW"
                     /locus_tag="J1Y83_002981"
     CDS             complement(12329..12832)
                     /gene="cheW"
                     /locus_tag="J1Y83_002981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HBA7631111.1"
                     /translation="MTGMTNVTKLASEPSGQEFLVFTLGDEEYGIDILKVQEIRGYDQ
                     VTRIANTPAFIKGVTNLRGVIVPIVDLRIKFSQVDVDYNDNTVVIVLNLGQRVVGIVV
                     DGVSDVLSLTAEQIRPAPEFAVTLSTEYLTGLGALGDRMLILVNIEKLLNSEEMALLD
                     SAASEVA"
     gene            complement(12853..14817)
                     /gene="cheA"
                     /locus_tag="J1Y83_002982"
     CDS             complement(12853..14817)
                     /gene="cheA"
                     /locus_tag="J1Y83_002982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheA"
                     /protein_id="HBA7631112.1"
                     /translation="MSMDISDFYQTFFDEADELLADMEQHLLVLQPEAPDAEQLNAIF
                     RAAHSIKGGAGTFGFSVLQETTHLMENLLDEARRGEMQLNTDIINLFLETKDIMQEQL
                     DAYKQSQEPDAASFDYICQALRQLALEAKGETPSAVTRLSVVAKSEPQDEQSRSQSPR
                     RIILSRLKAGEVDLLEEELGHLTTLTDVVKGADSLSAILPGDIAEDDITAVLCFVIEA
                     DQITFETVDVSPKISTPPVLKLAAEQAPTGRVEREKTTRSSESTSIRVAVEKVDQLIN
                     LVGELVITQSMLAQRSSELDPVNHGDLITSMGQLQRNARDLQESVMSIRMMPMEYVFS
                     RYPRLVRDLAGKLGKQVELTLVGSSTELDKSLIERIIDPLTHLVRNSLDHGIELPEKR
                     LAAGKNSVGNLILSAEHQGGNICIEVTDDGAGLNRERILAKAASQGLTVSENMSDDEV
                     AMLIFAPGFSTAEQVTDVSGRGVGMDVVKRNIQEMGGHVEIQSKQGTGTTIRILLPLT
                     LAILDGMSVRVADEVFILPLNAVMESLQPREADLHPLAGGERVLEVRGEYLPIVELWK
                     VFNVAGAKTEATQGIVVILQSGSRRYALLVDQLIGQHQVVVKNLESNYRKVPGISAAT
                     ILGDGSVALIVDVSALQAINREQRMANTAA"
     gene            complement(14822..15748)
                     /gene="motB"
                     /locus_tag="J1Y83_002983"
     CDS             complement(14822..15748)
                     /gene="motB"
                     /locus_tag="J1Y83_002983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor protein MotB"
                     /protein_id="HBA7631113.1"
                     /translation="MKNQAHPIIVVKRRKAKSHGAAHGSWKIAYADFMTAMMAFFLVM
                     WLISISSPKELIQIAEYFRTPLATAVTGGDRISNSESPIPGGGDDYTQSQGEVNKQPN
                     IEELKKRMEQSRLRKLRGDLDQLIESDPKLRALRPHLKIDLVQEGLRIQIIDSQNRPM
                     FRTGSADVEPYMRDILRAIAPVLNGIPNRISLSGHTDDFPYASGEKGYSNWELSADRA
                     NASRRELMVGGLNGGKVLRVVGMAATMRLNDRGPDDAVNRRISLLVLNKHAEQAILHE
                     NAESQNEPVSALEKPEVAPQVSVPTMPSAEPR"
     gene            complement(15745..16632)
                     /gene="motA"
                     /locus_tag="J1Y83_002984"
     CDS             complement(15745..16632)
                     /gene="motA"
                     /locus_tag="J1Y83_002984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor stator protein MotA"
                     /protein_id="HBA7631114.1"
                     /translation="MLILLGYLVVLGTVFGGYLMTGGSLGALYQPAELVIIAGAGIGS
                     FIVGNNGKAIKGTLKALPLLFRRSKYTKAMYMDLLALLYRLMAKSRQMGMFSLERDIE
                     NPRESEIFASYPRILADSVMLEFIVDYLRLIISGHMNTFEIEALMDEEIETHESEAEV
                     PANSLALVGDSLPAFGIVAAVMGVVHALGSADRPAAELGALIAHAMVGTFLGILLAYG
                     FISPLASVLRQKSAETSKMMQCVKVTLLSNLNGYAPPIAVEFGRKTLYSSERPSFIEL
                     EEHVRAVKNPQQQTTTEEA"
     gene            complement(16759..17337)
                     /gene="flhC"
                     /locus_tag="J1Y83_002985"
     CDS             complement(16759..17337)
                     /gene="flhC"
                     /locus_tag="J1Y83_002985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar transcriptional regulator FlhC"
                     /protein_id="HBA7631115.1"
                     /translation="MSEKSIVQEARDIQLAMELITLGARLQMLESETQLSRGRLIKLY
                     KELRGSPPPKGMLPFSTDWFMTWEQNVHASMFCNAWQFLLKTGLCNGVDAVIKAYRLY
                     LEQCPQAEEGPLLALTRAWTLVRFVESGLLQLSSCNCCGGNFITHAHQPVGSFACSLC
                     QPPSRAVKRRKLSQNPADIIPQLLDEQRVQAV"
     gene            complement(17340..17690)
                     /gene="flhD"
                     /locus_tag="J1Y83_002986"
     CDS             complement(17340..17690)
                     /gene="flhD"
                     /locus_tag="J1Y83_002986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416406.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar transcriptional regulator FlhD"
                     /protein_id="HBA7631116.1"
                     /translation="MHTSELLKHIYDINLSYLLLAQRLIVQDKASAMFRLGINEEMAT
                     TLAALTLPQMVKLAETNQLVCHFRFDSHQTITQLTQDSRVDDLQQIHTGIMLSTRLLN
                     DVNQPEEALRKKRA"
BASE COUNT         4402 a         5052 c         4456 g         4329 t
ORIGIN      
        1 ggcaatcgtt cgttttttat actgctcagg gataaaaaaa ggcccctgtt gaaattgcag
       61 gggcctggta cgagcaagca tcatattggg cgacatgatg caacggtaaa aatcatttgg
      121 cctgatggcg ttcaatgatt cctttcattt cagcaaccgc cgccccgtct acgacataac
      181 gcgaatactc gtccgcgtgt gcatctgatg acatggacaa tccttgatta cgataacgca
      241 tcggtgaggc ttgccacgct cccgcggagc tatggacttc cagcactccg gcatcgagga
      301 agtggtgcag gttttccgca cggactcctg ctccggccat aatgattgga gcatcacgat
      361 gggcaataag ttccataatt tttgataaac cttgcagtgc gtctgatttt tgccctgatg
      421 tcagtacccg ggtaatgccc aattccgcaa gattattgag tgtatttaaa gggttagcgc
      481 acatatcgaa ggcgcgatga aaagtcactg ccagcggacc ggcagcagcc attatttttt
      541 ccattcgtgg catatcgaca ttcccgtcaa catcgagaac gcccgtcacc agtccaggaa
      601 aacctaattc gcggactgtg cgcacatctt caagaatggc ggcaaactca ccgtcgctgt
      661 agcaaaaatc accaccgcgt gggcggataa tcggatgcac tgggatcgtc acccgctggc
      721 gcacggattt cagtaccccc aacgacggcg ttaagccccc ctcttttgga gctgcgcata
      781 attcaactct gtcagcgccg ttttgctgcg ccgttagcgc gcattccatg ctgtaacagc
      841 aaatttccag taatgccatt tttgctcctt agttacgccg actgctcgct ggcaacgatc
      901 tcttcaatgg accacggatg aaacttaatg gttgttttac catcggtgac cgccagcgtc
      961 ggattcggta atcgttcatg ctcacctttt ggcgaactgg ttttaactga gatccccggc
     1021 aggctgagtc cttcatcaga aagcaatgcc agcgcacggg cattcagcgt ttctggatcc
     1081 cccggcagaa cgatttcaat atgttcccaa ccttcgtgcg ggtaacgttt ttccccgggc
     1141 cacggtagct ccacaatgga aaactgccag tgcgcaacct gtaccggttc atgcaattta
     1201 aacagacaaa tcggtctgcc attgatcata ttttctgaca aaagctcgcc gcactgttca
     1261 aacccgcgac gccagcgttc agcggtgacg ttttgatggc aacgcaagga aatgtgatcg
     1321 gcagtcagcg gagtgatatc caaccccaga cggcgggaaa gttcatctaa tgcgtgggta
     1381 aatcgcggta aatccgatgc aatatcctgc agctcgtcga tagattgcca gttcgccata
     1441 atcactcttc gtctttcatt aaaagcgtta atttaccctg ttgccctgcg ccaaccaacc
     1501 gctgatttcg cgccgctcct gatgcaatag tgaaaacggc aatacgcccc gcgcacgttg
     1561 ctgacgaaaa cagccatttg cagtatactc ccgccctaat ttctttaact ggtgcgggca
     1621 atttttgctc gcttcatcaa tgtaaggtat tccggtgaat attcaggctc ttctctcaga
     1681 aaaagtccgt caggccatga ttgcggcagg cgcgcctgcg gattgcgaac cgcaggttcg
     1741 tcagtcagca aaagttcagt tcggcgacta tcaggctaac ggcatgatgg cagttgctaa
     1801 aaaactgggt atggcaccgc gacaattagc agagcaggtg ctgactcatc tggatcttaa
     1861 cggtatcgcc agcaaagttg agatcgccgg tccaggcttt atcaacattt tccttgatcc
     1921 ggcattcctg gctgaacatg ttcagcaggc gctggcgtcc gatcgtctcg gtgttgctat
     1981 gccagaaaaa caaaccattg tggttgacta ctctgcgcca aacgtggcga aagagatgca
     2041 tgtcggtcac ctgcgctcta ccattattgg tgacgcagca gtgcgtactc tggagttcct
     2101 cggtcacaaa gtgattcgcg caaaccacgt cggcgactgg ggcactcagt tcggtatgct
     2161 gattgcatgg ctggaaaagc agcagcaaga aaacgccggt gaaatggagc tggctgacct
     2221 tgaaggtttc taccgcgatg cgaaaaagca ttacgacgag gatgaagagt ttgccgagcg
     2281 tgcacgtaac tacgtggtaa aactgcaaag cggtgacgaa tatttccgcg agatgtggcg
     2341 caaactggtc gacatcacca tgacgcagaa ccagatcacc tacgatcgtc tgaacgtgac
     2401 gttgacccgc gatgacgtca tgggtgaaag tctctacaac ccaatgctgc cgggtatcgt
     2461 ggcggacctg aaagccaaag gtctggcagt agaaagtgaa ggcgcgaccg ttgtattcct
     2521 tgatgagttc aaaaacaagg aaggcgaacc gatgggcgtt atcattcaga agaaagatgg
     2581 cggctatctc tacaccacca ctgatatcgc ctgtgcgaaa tatcgttatg aaacactgca
     2641 tgccgatcgc gtgctgtatt acatcgattc ccgtcagcat caacacctga tgcaggcatg
     2701 ggcgatcgtc cgtaaagcgg gctatgtacc ggaatccgta ccgctggaac accacatgtt
     2761 cggcatgatg ctgggcaaag atggtaaacc gttcaaaacc cgcgctggcg gtacggtgaa
     2821 actggccgat ctgctggatg aagccctgga gcgtgcacgt cgtctggtgg cagaaaagaa
     2881 cccggatatg ccagccgacg agctggaaaa actggctaac gcggttggta ttggtgcggt
     2941 gaaatatgcg gatctctcca aaaaccgcac cacggactac atcttcgact gggacaacat
     3001 gctggcgttt gagggtaata ccgcgccata catgcagtat gcttacacgc gtgtattgtc
     3061 cgtgttccgt aaagcagaaa ttaacgaaga gcaactggct gcagctccgg taatcatccg
     3121 tgaagatcgt gaagcgcaac tggcagctcg cctgctgcag tttgaagaaa ccctcactgt
     3181 ggttgcccgt gaaggcacgc cgcatgtgat gtgtgcttac ctgtacgatc tggccggtct
     3241 gttctctggc ttctacgagc actgcccgat cctcagcgca gaaaacgaag aagtgcgtaa
     3301 cagccgtctg aaactggcac aactgacggc gaagacgctg aagctgggtc tggatacgct
     3361 gggtattgag acagtagagc gtatgtaatc gcgttatacg gcaacagccg atgtcattgc
     3421 agacatcggc ttttttatca ttaacgataa tttacaatca cttcattccg ctgaatcttt
     3481 agcggcggaa ttaaccgccc accgccaggc acttcccaga taaagcgcaa cggttctgcc
     3541 gccggtatac cggaaaaggc cacggtggtt ccactctgcc cctctaattc gacacaacga
     3601 gattgtgaac acaagcgaac ccgcagtcct gcaggtgtcg gaccgataag ctgataatgc
     3661 cacgccacca gcgtcatcaa tcctgaagca ggttgtcgcg tagaaagagg cgctgacgac
     3721 atcgattcac cgcgatgatt tagcgtaacg cccacgctac tcgcctgcca catcccctcc
     3781 ccggcggctt gcaccagcag cggaaacaat aatattgcta ataaggctct cattatttgc
     3841 cgccaatcgt cgccgtcata cggatatgtc ggttatctga tagttccaga ttcgacagca
     3901 ccactaactg cggcaagctg cggcgcagga agcgagacaa taatggtcgc agcgcgtggt
     3961 tcaccaacag taccggcggc gcacccaaca tctcctgacg ggatagcgct tcctgagttt
     4021 gcgccaacaa gcgatccgcc agccctggct ccagtccccc cccgccctgc aatgcctgta
     4081 gcaacaaacg ttccagcggt gtatcgaggc caataacatg gacttcatct ttgccaggaa
     4141 accactgctg ggtaatcgca cgtcccaacg ccacgcgcac gacagcggtt aattcatgcg
     4201 gatcgctctg gatgggcgca tgttccgcca gcgtttcgag aatggtgcgc atatcgcgaa
     4261 tcggcacttt ttcatcgagc agattttgca gcactttatg cagtgtggta agcgtgacga
     4321 cgccaggaac gagatcttcc gtcagctttg gcatctcctg ggcgacgcga tccagcagct
     4381 gttgcgcctc ctgacgacca aacagctctg cggcatgctg gctaatgagg tggttaagat
     4441 gcgttgccac caccgtgctg gcctcaacca cggtgtaccc ctgaatctgc gcctgctctt
     4501 taagcgcact ttcaatccag atagcattca ggccaaatgc cggatcgacg gtcgcctcac
     4561 caggtaacgt cccggcagcg gttccagggt taatcgccaa ccaacgcccc ggataagcat
     4621 caccactgcc tatctccacg cctttcatca aaatgcgata gccggcaggt tgcagatcca
     4681 tattgtcgcg aatgtgtacc actggcggca gaaaccccat ctcctgggca aatttcttgc
     4741 ggatactgcg tatacggccc aacaactcac catcctgctg gaaatcgacc atcgggatca
     4801 gtcgataacc cacttccatt cccagagaat cttccagttg tacatcgttc cacgtcgctt
     4861 cgacaacggt attattctct gccattttta ccggttttgg ttcagcaggc gatttttgtt
     4921 cacgtccgcg gatccaccag gccagcccca gcaatccggc agtgaacagc aaaaatacca
     4981 ggttcggcat tccaggcacc aggccgagta aaccgagcac ggcggcgctt aataacataa
     5041 cgctcggatt actgaaaagc tgattcacca tctgctcgcc aacatcctga tcggtgctga
     5101 cacgtgtaac gatgacgccc gcggcggtag aaatcaccag tgccgggatt tgtgccacca
     5161 gaccgtcgcc aatggtcaat agcgtataac tttccgccgc gtgccccatg ctcatgccat
     5221 gttgcagcac accgaccagc aatccgccga caacgttaat gaccatgatg aggatcccgg
     5281 cgatggcatc gccgcgaaca aacttacttg ccccgtccat cgagccgtaa aaatcggctt
     5341 cctgagtcac ttcggagcgg cgttttttcg cctcatcttc accaatcaat ccggcgttaa
     5401 ggtcggcgtc aatcgccatc tgcttacccg gcataccatc gagaacaaag cgcgcgccca
     5461 cttccgcaat acgcccggca cctttggtga taaccataaa gttgatgatc acgagaatga
     5521 cgaacaccac gataccgata gcgaaattgc caccaacgag gaagtgaccg aacgcttcga
     5581 ccacctttcc agccgccgcc gcgccggtat gcccttccat caaaatgata cgggttgaag
     5641 ccacgttaag tgccagacgc aacagcgtgg taaacaacag gatggtcgga aacgcagcaa
     5701 actcaagcgt gcgctgggta aacatcgcca ccagcaacac catgatcgac aaggcaatat
     5761 tgaaggtaaa cagcaggtcg agtatgaatg cgggcagtgg cagtaccatc atcgacaaga
     5821 tcaacaggat caaaatcggt ccggcaagga tctgccattg tgtcgatttc aggtttgcag
     5881 gcaggcgcag catcgcggcc agattactca tgggtcggtt tctcgttaat aaaatccagg
     5941 gcttccggca ccggaagatg agtaggttgt acagggcgtt gtccaccagc cagacgccag
     6001 cgtttcagtt gccagaccca ggccagcact tccgccaccg cggcgtacag ttgacccggg
     6061 atttgttgac cgatctccgc atgtcgatac agcgcacgcg ccagcggcgg cgcttcaagc
     6121 gtcgggacgt tattttcagc gccaatttca cgaatgcgca acgcgaccag ccctgcacct
     6181 ttagcgacca ctttcggtgc gctcattttg ttttcgtcat actgcaacgc taccgaatag
     6241 tgggtcgggt tattgacaat gacatccgct ttcggcacat cggccatcat ccgacgccgt
     6301 gcggccgctc gctgcatctg acggatccgc cctttaacat gggggtcgcc ttcgctttgt
     6361 ttgaactcat cacgaatatc ctgccgtgac attcgcagct ttttcaggtg gctgaagatt
     6421 tggaaaaaga cgtcaaatcc caccattgga atgacaccaa gcaccaccag cagcgcgcat
     6481 agccctacca gatccatcgc attacccatg gcggtaatcg gagactcggc cattaagcgc
     6541 atcatctgcg gccagtgatg ccagagaaaa aaccccgtca cgctgccaac caggatggtt
     6601 ttcaaaattg ccttaagcaa ctccgcgcca gtctgtgccg agaacatccg tttaatgccc
     6661 ggtagcgggt tgagtttgga aaacttcggc tgcaaggatt ttccgctaaa taccagcccc
     6721 cccagcataa ccggagaaat aagcgccacc agcaccacgc cgctaatcag cggtaacagc
     6781 gccagcatgg cttctctgat cagcagaata atctgcccga ggatcagatt cgggtcattg
     6841 ataatactgt gatcaaaatg cagccctgcg gagagcatgc ccgacaatcg acgggccagc
     6901 gacacaccgc caaaccagat aacactaacg cccaccagca aaatcagcag tgaagtcagt
     6961 tcacgggaac gcgggatttg cccctcttcc cgcgcttttt ctagtcggtg aggtgtgggg
     7021 gcttctgttt tgtcgtcgct ctcgtcagac acgtcgccaa tcctggaaag agtattaagc
     7081 gtaaatgatg ccagagcgca aagcgttcaa tggtttgagt aaggggcaaa acaggcggga
     7141 tttagggctt ttgccacagc acatcaggca tggtgacatg tttgtcgggt acggcgtaaa
     7201 cgccttatcc aaccttgaag atctgatgtg acgcggccac gtcacatcag gcaatacaaa
     7261 tcaaaatcca agactatcca acaaatcgtc cacctgatcc tgactggcta ccacaccggc
     7321 tttgctggta tcgacctgag gtccattaag caaactctgg ttttcacgtt ttggacgcga
     7381 ctcctgttcc gggatgtttt ccaacagcac catcagcaac tgacgttcga tctcctgaat
     7441 gacatccatc atccgcttaa tgacctgtcc ggtgagatcc tgaaaatcct gcgccatcat
     7501 gatttccagc agttgcgcgt tagtaaagtt ggtatgtgcg gggacatctg ccagaaattg
     7561 ccgcgtgtct gttaccagtt cacgggcgtc ggcaaggtca atcggatcgg caaaccagtc
     7621 atcccaacgt tgggttaacg cttttgctga tttctccatt tgatcctgat gcggttgtga
     7681 cgcctcaaca ctgttcagcg cccgctccgc agcctgggcg gtcatctgca caacatagta
     7741 caaacgatcg cgcgcatcgg ggatggcttc cgctgcttcg gcaatggcct gatccagccc
     7801 cagttcccgc aaactgtcgc gcagcatacg cgtcaggctg ccgatgcgcg caatgatatc
     7861 gccagctgaa tgctcgtcag caggtttgat tgatggttgc atcatagtcg catcctcaca
     7921 tgcccagttt ctcaaagatt ttgttgagtt tttcctccag cgtcgcggcg gtaaatggtt
     7981 tcaccacata gccactggcc cccgcttgcg ccgcagcaat gatgttctct ttcttcgctt
     8041 ctgcagtcac cattaacact ggcaatgccg acatcgcgcc atccgcacga atcgttttca
     8101 gcaactccag gccatccata ttgggcatgt tccagtcgga gataacaaat ccataaccgc
     8161 ctgcctgcaa cttattgaga gcgtcgaggc catcttccgc ttcctcaaca ttattgaatc
     8221 ccagctcttt cagcaggtta cgcactatgc gtcgcatggt ggaaaagtca tccacaacca
     8281 aaaatttaag ttctttatcc gccatttcac actcctgatt taaatacgta tcgcctgtcc
     8341 ggcactaatt tttgccagca tttgctgact tacctggcta agatcaacca cttcgcagac
     8401 accacccata ttgatggcct cgcgcggcat gccgaacacc acgcaacttg cttcgttttg
     8461 cgcaagggtc catgcccccg cctgacgcat cgccaacatt cccgccgcgc cgtcgttgcc
     8521 cataccggtc aggattaccc caaccgcatt acgccccgcc tgtttggcca cagaatggaa
     8581 caacacatct accgatggcc gatggcgatt aaccgccggg ccatcgtgaa ttttgatttg
     8641 ataatttgcg ccactacgcg ccagctccat atgccgatcg cccggcgcga tataggcatg
     8701 tcccggcaag acgcgctctc cgtcttcggc ttctttaacc ccgatctggc aaagcttatt
     8761 aagtctgtcg gcaaaagagc gggtgaaacc gggcggcata tgctgggtaa ttaacagtgc
     8821 cggactggaa agcggcaacg gttgcagtac gtgacgaatt gcctcagttc cacccgttga
     8881 agcaccaatc gcaatcagtt tttcagaact caacaacggc cccgccttca gcgttgtcgg
     8941 taccgacaat ggcttatgtg ctgcaaggct cgcctttgct gccgtacgca ccttttcagc
     9001 aatcatttcg ctatacgcca gcattccttc gcgaataccc agttgcggtt tggtgacaaa
     9061 atctatcgcc cccagctcca gcgcgcgcag cgtaacttct gaccctttgc cggtcaggga
     9121 agaaaccatc acaacgggca ttggacgcaa acgcattaat ttttcgagga aatccagtcc
     9181 gtccatccgc ggcatttcaa catccagcgt caatacatcg ggattgaatt tcttaatcaa
     9241 atcacgcgca accagcggat caggggcagt cgccaccatt tccatgtcgc tatggctgtt
     9301 gatgatttct gtcatgatct ggcgcatcag tgccgaatca tcgacagata acaccctgat
     9361 tttgctcatc gttaatcctt acttagcgca tacaccgtct gaccacgcag cgtgaagcgg
     9421 cgctcaaggt ggctaaagtt ttcagagtga cccgcaaaca gcaatccgtc gggtttaagg
     9481 agcgaaacaa agcggcgcaa aatctcctgc tgggtagttt gatcgaagta gatcatgacg
     9541 ttacgacaga agatcgcatc aaacggcccc ggcacggtgt actgtttcgc cagcaaattc
     9601 agcggggcaa aatcaacata gttcgccagc tcctgacgca cgcgtaccag cccttcatgc
     9661 ggtcccgtcc ctcgcatgaa ataccgttgc agttgctgcg gcgtcaggtt tttcaactct
     9721 tcatggcgat aaataccgct tctggctttt tccagcactt cggtgtcgat atcgctggca
     9781 aacactttcc agcgtccggg cgcggtgccc aatgtattag ccagcgtcat cgctatgctg
     9841 tacggctctt cgccggttga agccgccgcg ctccatacgc gatactcgcc agaacggcga
     9901 cgtgcgtgat ccgcgagcag aggaaaatgg tgtgcctcac ggaaaaatgc cgtcagattc
     9961 gtggtcagcg aattgataaa cgcctgccac tcaccgctgt gctgattaga ttccagcaag
    10021 ttcaggtaat gaccgaaatc cgccagtccc agcgcacgca aacgacgaac cagtcggttg
    10081 taaaccatgt cgcgtttatg gtcagccaga acgatcccgg ctcgttgata gatcaattga
    10141 cttatccgcc gaaaatgcgc gtcggaaagc gccaggcgct cggtcatctg taacaataaa
    10201 gacatttgcc cacagggcag agatgaagtc atagcacctt ctcaatcact tcaggatacc
    10261 actggcgcaa tttgtaactg cgccgactca tgtcgtgcta cttcatgttc ttcaagggta
    10321 aataccgcca cacgcgacga aagatggtcg gcctgattca gtcgttgcaa atcgtcgcga
    10381 aatgcccaaa agacatgccg ttactgccaa tctgcaagat cccgcagaga atcaaaatta
    10441 aaaacagcgt ggtcgaaatt cgaatacgat taaacatcaa cgctccccat caggcggcaa
    10501 tgaccgcttt agtaaatact cgtcaaaatg tttcccagtt tggatcttgt tcagcaattc
    10561 gcagtcgtgg ctgtgccggt ggttgctcac tggcaggacg ggatggtgtt tgcggtttat
    10621 tggtgagtgg gctggctgcc agacggaacg cggaaaccgc ttgcgttaaa cgactcgcct
    10681 gttcttccag cgcagctgcg gcggcagctg attcctgcac cagcgatgcg ttctgttgcg
    10741 tgacgcgatc catttccgaa accgccaatg cgacttgatc gatgccacgg ctctgttcat
    10801 ccgatgctga tgcaatctcg cccataatgt cagtcacgcg agtgacagca ttgacgatat
    10861 tgttcattgt ttccccagcg ctttcgacca gcaccgaacc ggtatcaacg cgcgagacgg
    10921 agtcttcaat gagggctttg atctcttttg ccgcctgtgc actgcgactg gcaagattac
    10981 gcacttcacc cgccaccacg gcaaaaccac ggccctgttc acccgctcgc gcggcttcaa
    11041 cagcggcatt cagcgcgagg atattagtct ggaaggcaat accatcgata acgctgataa
    11101 tgtcggcaat tttcttcgaa ctatcggcga tctcatgcat cgttttcact acgccatcca
    11161 ccactttgcc gccgtgctgg gcggtgtcgg aggcactttg cgccagttgc gaggcctggc
    11221 gggcgttatc ggcgttttgc ttcactgtcg cggtgagctg ctccatgctg gcggcagttt
    11281 cttccagcgc ggatgcctgc tgttcagtac gggaggaaag atcggtgttg cccgccgcaa
    11341 tttcacgggt accggcatag atggcatctg aaccttggcg cacatgagtg acggtgtcag
    11401 tcaaagagcg ttgcatatgt gaaacgctct gcgccaggtc gcccatttca ctgcgcccgt
    11461 caatggtcag ggtattcgcc aggttaccac cggcaatttc gcgaatatgc gcaataattt
    11521 ttgccagcgg tgtaagcaac atacggcgaa tgccgtacca cgccaccagc agaatcaata
    11581 ccaccaccag cgcgataacc gccagttgcc actgggcaaa tcggtaatca tctgcgttgt
    11641 cagtgacgat atcgcgatac agtttttcac tgctgagggc gtactgagca aacgcttcgc
    11701 ccattgcatt ttgcattccc tgggttggct gagcgaaata agctccagta ttgccataat
    11761 caagataatc aatcagttca gttaacgctg tgtgatagtt tttatatttt tcatcaatat
    11821 tacgactggt agcgaccatt tcaggtaacg gtgccatgct tttgaatttt ttataatgag
    11881 tcgcggcctg ggccaatgtt ttcctggcac tatcgagcaa ctcaactttg gcattacttt
    11941 gcttattgga tgaatccatc atcatccgca ccgctgaacg actcaggtta atgcgcgttt
    12001 gtagcattaa atcccatgtt gacgtcagct cgccctgctg ttcccgtaat tgattggaaa
    12061 ccacaaagct cttctggcta tggtgaaggg aagaaaaaaa cagactgccg gaaataagct
    12121 gtaacagtgc gaataccccc agcaccatta ccaacagcgt gactacgcgg atacggttaa
    12181 tcataaggca ccttcctgaa aacaagttga tctcgttatc ggcaaggagg ggggaaactt
    12241 tattgctgat gccacccgcc gcgaaattga aataaaaaac ccgatgcgca gatcatcggg
    12301 ttcatttcaa ttgaggaaat cgggagaatt acgccacttc tgacgccgcg ctatctaaca
    12361 gcgccatctc ttcgctgttc agcagttttt cgatgttcac cagaatcaac atgcggtcgc
    12421 ccagtgcgcc cagtcctgtg agatattcgg ttgaaagcgt cacggcaaat tccggtgccg
    12481 gacgaatttg ctccgccgtc aatgaaagca cgtctgagac gccgtcaacc acgatgccga
    12541 ccacccgctg tccgagattc aggacgataa ctaccgtgtt gtcgttatag tccacatcca
    12601 cctggctgaa cttaattcgt aagtcaacaa tcggcacaat aacgccgcgc agattcgtga
    12661 cgcctttgat aaacgctggc gtgttcgcaa tccgtgtcac ctgatcgtag ccacggatct
    12721 cctgcacttt caggatatca ataccgtact cttcatcacc aagggtaaat accagaaatt
    12781 cctggcctga cggctcgctg gccagctttg ttacattcgt cataccggtc atattgttac
    12841 ctttttactc attcaggcgg cggtgttcgc catacgttgt tcgcggttta tcgcctgcaa
    12901 ggcggagaca tcaacaatca gtgccacgct gccgtcgcca agaatggtcg cagcagaaat
    12961 gccggggact ttgcgatagt tactttcaag gtttttaacc acaacctggt gttgaccaat
    13021 taattgatcc accagcaagg cgtagcggcg actgccactt tgtaagatca ccacaattcc
    13081 ctgggtagct tcggttttcg cgcccgcgac gttgaacact ttccacagtt cgacgatggg
    13141 cagatattca ccccgcactt ccagcacccg ctcgccgcca gccagtggat gcagatcggc
    13201 ttcacggggt tgcagtgatt ccatgacagc attcagcggc agaatgaaaa cttcatccgc
    13261 aacgcgtacg gacatgccgt caagaattgc cagcgtcagc ggcagtaaaa tgcggatcgt
    13321 agtgccagta ccctgcttcg actggatctc gacatgaccg cccatctcct ggatattacg
    13381 tttaacgacg tccatgccga cgccacgccc ggagacgtcg gtgacctgct ccgccgtgga
    13441 gaagccaggg gcaaatatca gcattgcaac ttcgtcgtcg ctcatgtttt cgctgacggt
    13501 cagaccttgc gaggccgctt ttgccagaat tcgttcacgg tttagccccg ccccatcgtc
    13561 ggtcacttca atgcaaatgt tgccgccctg atgttcggca gacagaatta aatttccgac
    13621 gctgttttta cctgcagcga gccgtttttc tggcagttca ataccgtgat cgaggctatt
    13681 gcgtaccagg tgggtcagcg ggtcgataat gcgttcaatc aggctcttgt cgagttcagt
    13741 agaactgccc accagcgtca gttctacctg cttgccgagt tttcccgcca gatcacgcac
    13801 cagccgggga tagcgactaa agacatattc catcggcatc atgcgaatcg acatcactga
    13861 ttcctgcaaa tcacgggcgt tacgttgtaa ctgccccatg ctggtaatca aatcaccatg
    13921 attaaccggg tccagttcgc tggaacgctg ggcaagcatg gactgggtga tgaccagctc
    13981 accgacgagg ttaattaatt gatcaacctt ttctaccgct acacggatgc tggtggattc
    14041 actgctgcgc gtcgtttttt cccgctccac gcgaccggtt ggcgcttgtt cggctgccag
    14101 tttaagcact ggtggggtgg atatttttgg cgagacgtct actgtttcaa aggtaatctg
    14161 atcggcttca atcacaaaac agagtaccgc tgtgatgtca tcttcggcga tgtcgcccgg
    14221 taatattgcc gagagagaat ccgccccttt caccacgtca gttaacgttg tcagatgccc
    14281 caactcttct tccagcaggt cgacttcgcc ggccttcagg cgcgaaagga taattcgtcg
    14341 cggtgactga ctacgactct gctcatcttg cggttcactt ttggcaacca cacttaatcg
    14401 ggtcactgca gatggcgttt cgcctttcgc ttctaatgcc agttgacgca aggcctggca
    14461 gatataatcg aagctggcgg catccggctc ttgcgactgt ttataagcgt cgagctgttc
    14521 ttgcatgatg tccttcgttt ccaaaaacag attgataatg tcggtgttga gttgcatctc
    14581 acctcgtctg gcttcatcga gcaggttttc catcagatgc gtggtttcct gcaaaacgct
    14641 gaagccaaag gttcctgccc ctcctttgat cgagtgggca gcccgaaaga tggcattcaa
    14701 ctgttcggca tctggcgctt ccggctgcaa aaccagcaga tgctgctcca tatcagccaa
    14761 cagttcgtcc gcttcatcaa aaaatgtctg ataaaaatcg cttatatcca tgctcacgct
    14821 gtcacctcgg ttcggctgat ggcattgtgg gaacactgac ctgtggtgca acctcaggtt
    14881 tttccagggc gcttactggc tcattctggc tttcggcgtt ttcatgcaaa atggcctgtt
    14941 cggcgtgttt gttcagtacc agcaggctga tgcgacggtt gacggcatca tcaggtccgc
    15001 gatcgtttaa gcgcatcgtt gccgccatgc cgacgacacg taacactttg ccgccattca
    15061 acccgccaac catcagttcg cggcgggatg cattggcccg atcggcagaa agctcccagt
    15121 tgctatatcc tttctcacca ctggcgtaag ggaaatcatc ggtatgaccc gaaaggctaa
    15181 tacggttggg aataccattc agaacgggcg cgatggcgcg cagaatgtcg cgcatatagg
    15241 gttcgacgtc ggcactgccg gttctgaaca tcgggcgatt ctggctatcg atgatctgaa
    15301 tacgtagacc ttcctggacc agatcgattt tgagatgtgg acgtaacgcc cgcagtttcg
    15361 gatcggactc tatcaattgg tcgagatcgc cacgcaattt ccgcaatcga ctttgctcca
    15421 tgcgtttttt cagctcttcg atgttcggct gcttattcac ttccccctgg ctttgggtgt
    15481 aatcatcacc accgccggga attgggcttt cactattaga aatgcgatcg ccgcccgtaa
    15541 ccgctgtcgc cagcggagtc cggaagtact ccgcaatctg aatcagctct tttggactgg
    15601 agatagagat cagccacatc accagaaaaa aggccatcat tgcggtcata aagtcggcat
    15661 aagcaatctt ccacgatcca tgtgctgccc cgtggctttt ggctttgcgt cgtttgacga
    15721 caataatcgg atgcgcttga ttcttcatgc ttcctcggtt gtcgtctgtt gttgcggatt
    15781 tttcaccgca cgcacatgct cttccagctc aataaacgac ggacgttcgc tggaatagag
    15841 cgttttgcga ccaaactcaa cggcgatagg cggtgcgtaa ccgttcagat ttgaaagcag
    15901 agtgactttg acgcactgca tcattttgct ggtttcggcg cttttctgac gtaaaacact
    15961 cgctaatggg gaaataaatc cgtaagccaa taaaataccg aggaaagtcc ccaccatcgc
    16021 atgagcgata agcgccccca gctcggcggc aggacgatcg gctgaaccta acgcgtgaac
    16081 gacccccatt acagccgcaa caataccaaa cgccggaagt gagtccccga ccagcgccag
    16141 actgttcgcc gggacttctg cctcgctttc gtgcgtctca atctcttcat ccatcagagc
    16201 ttcgatttcg aaggtgttca tgtgaccact gataatcagg cgcagataat cgacgataaa
    16261 ttcaagcatg acgctatccg caaggatgcg tgggtagctg gcgaagatct cgctctcacg
    16321 gggattttca atatcgcgtt ccagcgaaaa catccccatc tgccgcgatt tcgccatcaa
    16381 ccgataaagc agagccagca gatccatata cattgctttg gtgtatttgg agcgacgaaa
    16441 cagcaacggc agcgccttca gcgtgccttt aatcgctttg ccattattgc cgacgataaa
    16501 cgacccaatc cctgcaccgg caataatcac cagttcagcg ggttgataga gtgctccaag
    16561 gcttccaccg gtcatcaaat aaccgccgaa aactgtaccg agaacaacca ggtaacctaa
    16621 taagataagc acgacatcat ccttccactg ttgaccatga caggatgttc agtcgtcagg
    16681 cgttaacacg cgataggggc aaaaaaaagc agcggtacgt cgttaccgct gctggaatgt
    16741 ttcgccacac cgtatcagtt aaacagcctg tactctctgt tcatccagca gttgtgggat
    16801 aatatcggca ggattctggg aaagtttacg tctttttact gcccgggatg gcggctgaca
    16861 taagctgcag gcaaagctgc caacaggctg gtgagcgtgg gtaataaaat tgccgccgca
    16921 gcagttgcag ctggaaagtt gcagtaatcc actttcaaca aaccgcacca atgtccaggc
    16981 acgggttaat gccagcagcg gtccttcttc tgcttgcggg cactgttcaa ggtataaacg
    17041 gtaggctttg atcaccgcat cgacgccatt acacaagcca gttttcagta aaaactgcca
    17101 tgcattacag aacatcgaag catgaacgtt ttgttcccag gtcataaacc agtcggttga
    17161 gaatggcagc atgcctttcg gcggtgggct tccgcgcagt tctttataga gttttatcag
    17221 acgtccgcga cttaactgtg tttcgctttc cagcatctgc aaacgagcgc ccagggtgat
    17281 caattccatt gccagctgaa tatcccgcgc ttcctgaaca atgctttttt cactcatgat
    17341 caggcccttt tcttgcgcag cgcttcttca ggctgattaa catcattcag caagcgtgtt
    17401 gagagcatga tgccggtatg aatttgctgg agatcgtcaa cgcgggaatc ttgcgtcaac
    17461 tgagtaatcg tctggtggct gtcaaaacgg aagtgacaaa ccagttgatt ggtttctgcc
    17521 agcttaacca tttgcggaag agtcagtgcc gctaacgttg tcgccatttc ttcatttatg
    17581 ccgagacgaa acatagcgga cgctttgtcc tgaacaatca gacgctgtgc aagtagtaaa
    17641 tatgacaagt tgatgtcata aatgtgtttc agcaactcgg aggtatgcat tattcccacc
    17701 cagaataacc aactttattt ttatgcggtc tcaccgcacc ccgtgatgtc gccgggaagc
    17761 cccggtaaaa aataattagc attagaatag ttgcgataag ctgcaataag cagacccacc
    17821 tttttggttt aataagtcct tacaaataga agggggtctt tacacttatc taagattttt
    17881 cctaaatcga cgcaactgta ctcgtcacta cacgcacata caacggaggg gggctgcgat
    17941 tttcaataat gtgtgatgca gatcacacaa aacgctcaat tacttaacat aaatgtataa
    18001 gtcatacttt tgttttgggt gtatttcaat ctgttaaaaa ttttttcgct acactggcaa
    18061 gcaaaaatga aacaggaata atcgaaatgg gatgttgcgc acagttaaaa taactcaccg
    18121 taaataatca tctgctataa ataatcgctt taatgcaata ccagataagc catttttaaa
    18181 caaacactta ccgcacaaca aactaattaa ctacaaagaa aggcaacaat caacctaat
//
LOCUS       DADSYM010000102        18178 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2415
            SAMN08039579-rid11723173.denovo.102, whole genome shotgun sequence.
ACCESSION   DADSYM010000102 DADSYM010000000
VERSION     DADSYM010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039579
            Sequence Read Archive: SRR6362533
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18178)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18178)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980162.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 03:39:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,484
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 4,347
            CDSs (with protein)               :: 4,347
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 43 of 85
            Pseudo Genes (incomplete)         :: 37 of 85
            Pseudo Genes (internal stop)      :: 19 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18178
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039579-rid11723173.denovo.102"
                     /strain="LGE2415"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8302 N 95.9628 W"
                     /collection_date="2015-08-24"
     gene            complement(<1..2039)
                     /locus_tag="J1Y83_002987"
     CDS             complement(<1..2039)
                     /locus_tag="J1Y83_002987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HBA7631117.1"
                     /translation="MSQPVGDLVIDLSLDAVRFDEQMSRVRRHFSGLDTDARKTASAV
                     EQGLSRQALAAQKAGISVGQYKAAMRTLPAQFTDIATQLAGGQNPWLILLQQGGQVKD
                     SFGGMIPMFRGLAGAITLPMVGVTSLAVATGALAYAWYQGDSTLSAFNKTLVLSGNQS
                     GLTADRMLTLSRAGQAAGLTFNQASESLAALVNAGVRGGEQFDAINQSVARFASASGV
                     EVDKVAEAFGKLTTDSTSGLIAMARQFRNVTAEQIAYVAQLQRSGDEAGALQAANDIA
                     TKGFDEQTRRLKENMGTLETWADKTGKAFKSMWDAILDIGRPESSADMLASAQKAFDE
                     ADKKWQWYQSRSQRRGKTSSFRANLQGAWNDRENARLGLAAATLQSDMEKAGELAARD
                     RAERDASQLKYTGEAQKGYERLLTPLEKYTARQEELNKALKDGKILQADYNTLMAAAK
                     KDYEATLKKPKQSGVKVSAGERQEDSAHAALLTLQAELRTLEKHAGANEKISQQRRDL
                     WKAESQFAVLEEAAQRRQLSAQEKSLLAHKDETLEYKRQLAALGDKVTYQEHLNALAQ
                     QADKFAQQQRAKRAAIDAKNRGLTDRQAAWEATEQRLKEQYGDNSLALNNVMSEQKKT
                     WAAEDQLRGSWMAGLKSGWSEWEESATDSMSQVKSAATQTFDGIAQNMAAMLTGS"
     gene            complement(2020..2433)
                     /locus_tag="J1Y83_002988"
     CDS             complement(2020..2433)
                     /locus_tag="J1Y83_002988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein T"
                     /protein_id="HBA7631118.1"
                     /translation="MGRPDWRAMLAGMTSTEYADWRHFYRMHYFHDTQLDMHFSGLTY
                     AVLSLFFCDPDMHPSDFSLLVPRHEEEQVERPDEDKMLMQKAAGLAGGVRFGGDGGRD
                     ILSSADVADVMVDDAALMMASAGISGGVRYVPAGW"
     gene            complement(2460..2891)
                     /locus_tag="J1Y83_002989"
     CDS             complement(2460..2891)
                     /locus_tag="J1Y83_002989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein G"
                     /protein_id="HBA7631119.1"
                     /translation="MFLKTEQFEYNGVSVTLSELSALQRIEHLALLKRRAEQAESSGN
                     LQVSVEDLVRTGAFLVAMSLWHNHPQKTGSPSMNEAVMQIEQEVLTTWPADAIARAED
                     VVLRLSGMSGPVHVDTDITEVAKNNALTDDDFSAGKSSTAS"
     gene            complement(2905..3657)
                     /locus_tag="J1Y83_002990"
     CDS             complement(2905..3657)
                     /locus_tag="J1Y83_002990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBA7631120.1"
                     /translation="MPTPNPLAPVKGAGTTLWVYTGKGDAYANPLSDDDWQRLAKVKD
                     LTPGEMTAESYDDNYLDDEDADWSATGQGQKSAGDTSFTLAWKPGEEGQKGLIGWFES
                     GDVRAYKIRFPNGTVDVFRGWVSSIGKAVTAKEVITRTVKVTNVGKPSVAEERSEITP
                     ATAIKVTPTSGTVEKGKTTTLTVSFEPESATDKTFRAVSADPSTGTIAVKDMVITVTG
                     VKAGKVSIPVISGNGQFATVAEVTVTEAGAAG"
     gene            complement(3665..4060)
                     /locus_tag="J1Y83_002991"
     CDS             complement(3665..4060)
                     /locus_tag="J1Y83_002991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBA7631121.1"
                     /translation="MKHTDIRAAVLDALELHEHGATLFDGRPVVFDEEDFPAVAVYLT
                     DAEYTGEELDADTWRATLHIEVFLPAQVPDSELDSWMESRIYPAMSAIPALAGMITTM
                     VQQGYDYRRDDDMALWSSADLTYSITYEM"
     gene            complement(4057..4590)
                     /locus_tag="J1Y83_002992"
     CDS             complement(4057..4590)
                     /locus_tag="J1Y83_002992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBA7631122.1"
                     /translation="MAIKGLDQAIENLSRVRKNAIPAASAMAINRVATTAINQSSSQV
                     ARETRVSRKLVKERSRLKRATVRNPNARIIVNRGDLPVIKLGIRMPGRRPDSILKAGQ
                     HRYQRAFIQRLKNGRWHVMQRVAGKNRYPIDVVKIPMAAPLKQAFDENVDRIRRERLP
                     GELAYALKQQLRIAIKR"
     gene            complement(4605..4958)
                     /locus_tag="J1Y83_002993"
     CDS             complement(4605..4958)
                     /locus_tag="J1Y83_002993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001204533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBA7631123.1"
                     /translation="MRDFQNAFDAALAGVDSTIVEVMGLCAQFTSGAQCGSEVQGVFD
                     DPESLGFAGSGVRIEGSCPSLFVRTDTVRAVRRGDTLTINGETFWVDRVSPDDGGSCY
                     LWLNRGQPPAVNRRR"
     gene            complement(4951..5334)
                     /locus_tag="J1Y83_002994"
     CDS             complement(4951..5334)
                     /locus_tag="J1Y83_002994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001373367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein FI"
                     /protein_id="HBA7631124.1"
                     /translation="MATKEENQKRLRQLAGLLGREADMSGSAADIAQRVSEWEEELAA
                     SREGIMPGDESGPEQNHTDDGEQLHNTDATDDVKAVRVRQCLHVMGYCPETGRPVELT
                     YRGMRVLVPSPLATAMIQHGTAEHA"
     gene            complement(5386..6414)
                     /locus_tag="J1Y83_002995"
     CDS             complement(5386..6414)
                     /locus_tag="J1Y83_002995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major capsid protein"
                     /protein_id="HBA7631125.1"
                     /translation="MGLFTTRQLLGYTEQKVKFRALFLELFFRRTVNFHTEEVMLDKI
                     TGKTPVAAYVSPVVEGKVLRHRGGETRVLRPGYVKPKHEFNYQQAVERLPGEDPAQLN
                     DPAYRRLRIITDNLKQEEHAIVQVEEMQAVNAVLYGKYTMEGEQFDTVEVDFGRSEGN
                     NIEQADGKKWSEQDRDTFDPTHDIDLYCDQASGLVNIAIMDGTVWRLLNGFKLFREKL
                     DTRRGSNSQLETAVKDLGAVVSFKGYYGDLAIVVAKTSYVAEDGTEKRYLPEGTLVLG
                     NTAADGIRCYGAIQDAQALSEGVVASSRYPKHWLTVGDPAREFTMTQSAPLMVLPDPD
                     EFVVVQVK"
     gene            complement(6472..6819)
                     /locus_tag="J1Y83_002996"
     CDS             complement(6472..6819)
                     /locus_tag="J1Y83_002996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head decoration protein"
                     /protein_id="HBA7631126.1"
                     /translation="MVTKTITEQRAEVRIFAGNDPAHTATGSSGISSATPALTPLMLD
                     EATGKLVVWDGQKAGSAVGILVLPLEGTETVLTYYKSGTFATEAIRWPDSVDEHKKAN
                     AFAGSALSHAALP"
     gene            complement(6856..8361)
                     /locus_tag="J1Y83_002997"
     CDS             complement(6856..8361)
                     /locus_tag="J1Y83_002997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HBA7631127.1"
                     /translation="MRRNLSHIIAAAFNEPLLLEPAYARVFFCALGREMGAASLSVPQ
                     QQVQLDAPGMLAETDEYMAGGKRPARVYRVVNGIAVLPVTGTLVHRLGGMRPFSGMTG
                     YDGIVACLQQAMADSQVQGVLLDIDSPGGQAAGAFDCADMIYRLRQQKPVWALCNDTA
                     CSAAMLLASACSRRLVTQTSRIGSIGVMMSHVSYAGHLAQAGVDITLIYSGAHKVDGN
                     QFEALPAEVRQDMQQRIDAARRMFAEKVAMFTGLSVDAVTGTEAAVFEGQSGIDAGLA
                     DELVNASDAISVMATALNSNVRGGTMPQLTATEAAAQENQRVMGILTCQEAKGREQLA
                     TMLAGQQGMSVEQARAILAAAAPQQPVASTQSEADRIMACEEANGREQLAATLAAMPE
                     MTVEKARPILAASPQADAGPSLRDQIMALDEAKGAEAQAEQLAACPGMTVESARAVLA
                     AGSGKAEPVSASTTALFERIMANHSPAAVQGGVPQTSADGDADVKMLMAMP"
     gene            complement(8351..9943)
                     /locus_tag="J1Y83_002998"
     CDS             complement(8351..9943)
                     /locus_tag="J1Y83_002998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HBA7631128.1"
                     /translation="MKRTPVLIDVNGVPLRESLSYNGGGAGFGGQMAEWLPPAQSADA
                     ALLPALRLGNARADDLVRNNGIAANAVALHKDHIVGHMFLISYRPNWRWLGMRETAAK
                     SFVDEVEAAWSEYAEGMSGEIDVEGKRTFTEFIREGVGVHVFNGEIFVQPVWDTETTQ
                     LFRTRFKAVSPKRVDTPGHGMGNRFLRAGVEVDRYGRAVAYHICEDDFPFSGSGRWER
                     IPRELPTGRPAMLHIFEPVEDGQTRGANQFYSVMERLKMLDSLQATQLQSAIVKAMYA
                     ATIESELDTEKAFEYIAGAPQGQKDNPLINILEKFSSWYDTNNVTLGGVRIPHLFPGD
                     DLKLQTAQDSDNGFSALEQALLRYIAAGLGVSYEQLSRDYSKVSYSSARASANESWRY
                     FMGRRKFIAARLATQMFSCWLEEALLRGIIRPPRARFDFYQARSAWSRAEWIGAGRMA
                     IDGLKEVQESVMRIEAGLSTYEKELALMGEDYQDIFRQQVRESAERQKAGLSRPVWIA
                     QAYQQQIAESRRPEEETTPRET"
     gene            complement(9940..10146)
                     /locus_tag="J1Y83_002999"
     CDS             complement(9940..10146)
                     /locus_tag="J1Y83_002999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBA7631129.1"
                     /translation="MVTVAELQALRQARLDLLTGKRVVSVQKDGRRIEYTAASLDELN
                     RAINDAESVLGTTRRRRRPLGVRL"
     gene            complement(10130..12058)
                     /locus_tag="J1Y83_003000"
     CDS             complement(10130..12058)
                     /locus_tag="J1Y83_003000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit family protein"
                     /protein_id="HBA7631130.1"
                     /translation="MNISEQQLNNMMSAVTTALQPLIRALPVTPVEWADQNYYLPKES
                     SYGEGEWKTLPFQIAIMNSMGNDQIRTVNLIKSARVGYTKMLLGVVGYFIEHKSRNSL
                     LFQPTDSAAEDFMKSHVEATIRNVPCLKDLSPWLGRKHRDNTLTLKRFSSGVGFWCLG
                     GAAAKNYREKSVDVVCYDELSSFEPDVEKEGSPTLLGDKRIEGSVWPKSIRGSTPKIK
                     GSCQIEKAANESAHFMRFYVPCPHCGEEQYLKFGDDASPFGLKWEKNKPESVFYLCEH
                     HGCVIHQSELDQSNGRWICENTGMLTRDGLMFFSARGDEIPPPRSITFHIWTAYSPFT
                     TWVQIVYDWLDALKDPNGLKTFVNTTLGETWEEAVGEKLDHQVLMDKVVRYTAAVPAR
                     VVYLTAGIDSQRNRFEMYVWGWAPGEEAFLVDKIIIMGRPDEEETLLRVDAAINKKYR
                     HADGTEMTISRVCWDTGGIDGEIVYQRSKKHGVFRVLPVKGASVYGKPVITMPKTRNQ
                     RGVYLCEVGTDTAKEILYARMKADPTPVDEATSYAIRFPDDPEIFSQTEAQQLVAEEL
                     VEKWEKGKMRLLWDNKKRRNEALDCLVYAYAALRVSVQRWQLDLAVLAKSREEETTRP
                     TLKELAAKLSGGVNGYSR"
     gene            complement(12030..12539)
                     /locus_tag="J1Y83_003001"
     CDS             complement(12030..12539)
                     /locus_tag="J1Y83_003001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase"
                     /protein_id="HBA7631131.1"
                     /translation="MATQTEVARHLSLTDRQLRRLQKLPGAPVSNKRGQLDLDAWRDF
                     YISYLRRSKNDVPDGDSEEDYEEKLLIARWELTAEQAVTQQLKNEVSKGKLIDTGFCI
                     FALSKLAMALSSTLDSIPLSMQRQFPDLTPRHLDHLKTLIAKGANQCARAGDKLPDLL
                     DEYIRATTE"
     gene            12934..13128
                     /locus_tag="J1Y83_003002"
     CDS             12934..13128
                     /locus_tag="J1Y83_003002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001307652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3950 domain-containing protein"
                     /protein_id="HBA7631132.1"
                     /translation="MSTKNRTRRTTTRNIRFPNQMIEQINIALEQKGSGNFSAWVIEA
                     CRRRLTSEKRAYTSIQSDDG"
     gene            complement(13316..13933)
                     /locus_tag="J1Y83_003003"
     CDS             complement(13316..13933)
                     /locus_tag="J1Y83_003003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000881338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA7631133.1"
                     /translation="MLASLGGLVSYAAKVHFKEAGNAMNELIANHDFDFRQLVTAAEG
                     QPVTDTFQIARAFGKRHQHVIRAIKCLRCSEEFSTTHFWAVEKINDLGIFDKKQIYYR
                     MDFSGFVMLVMGFNGAKADAVKEAYINAFNWMSAELRKYSESYEAERNAVMLEYMKEK
                     DVASMSGRLLNRWGRTKKPQLLAKLERLERQGQFLLPGFDKGIQA"
     gene            complement(14083..14520)
                     /locus_tag="J1Y83_003004"
     CDS             complement(14083..14520)
                     /locus_tag="J1Y83_003004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009453323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HBA7631134.1"
                     /translation="MSRVTVIISALVICIIACLSWAVNLYRDNAIAYKEQRDKATSII
                     ADMQKRQRDVAELDARYTKELADANATIESLRADVSAGRKRLQVSATCAKSTTGASSM
                     GDGESPGLTADAELNYYRLRGGIDKITAQVNYLQEYIRTQCLK"
     gene            complement(14517..15014)
                     /locus_tag="J1Y83_003005"
     CDS             complement(14517..15014)
                     /locus_tag="J1Y83_003005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HBA7631135.1"
                     /translation="MSPSLRKAVAAAIGGGAVAIASVLITGPGGNDGLEGVSYIPYKD
                     IVGVWTVCHGHTGKDIMPGKTYTEAECKALLNKDLATVARQINPYINVDIPETTRGAL
                     YSFVYNVGAGNFRTSTLLRKINQGDIKSACDQLRRWTYAGGKQWKGLMTRREIEREVC
                     LWGQQ"
     gene            complement(15014..15220)
                     /locus_tag="J1Y83_003006"
     CDS             complement(15014..15220)
                     /locus_tag="J1Y83_003006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holin"
                     /protein_id="HBA7631136.1"
                     /translation="MEKITTGVSYTTSAVGTGYWLLQLLDKVSPSQWVAIGVLGSLLF
                     GLLTYLTNLYFKIKEDKRKAARGE"
     gene            complement(15791..17518)
                     /locus_tag="J1Y83_003007"
     CDS             complement(15791..17518)
                     /locus_tag="J1Y83_003007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000023217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1737 domain-containing protein"
                     /protein_id="HBA7631137.1"
                     /translation="MSIKHYDVVRAASPSDLAEKLTHKLKEGWQPYGGPVAITPYTLM
                     QAVAIEGEPQVGPSSEPDWYYVIVLAGQSNAMAYGEGLPLPDSYDAPDPRIKQLARRS
                     TVTPGGAACRYNDIIPADHCLHDVQDMSTLNHPRADLSKGQYGCVGQGLHIAKKLLPY
                     IPNNAGILLVPCCRGGSAFTQGAEGTFSESTGASQDSARWGVGKPLYQDLISRTKAAL
                     QKNPKNVLLAVCWMQGEFDMSAATHAQQPALFTAMLTQFRADLSVFNAQCHGGSAADV
                     PWVCGDTTYYWKNTYATQYDTVYGGYKNRESEGVYFVPFMTDGNGVNTATNAPAEDPD
                     IPASGYYGAASRTNGNQVSSNRPTHFSSWARRSIIPDRLATAILNAAGRTSAFISGKA
                     PEIKPSPGGNTPSGPSADTSVRTISLLPAAGEAAAQGWSIKDGGIQLSDGVFKITKQS
                     NKTWSLTHPVDDAITLLTQGGRLTCKFRLSGALTNNQFGLGIYLYTDAPVPDGVAMTG
                     TGNPFLMSYFTQTTDGRVNLMHHRKAGNTKLGEFGDYGNDWQTLELVFTAGSATVTPK
                     LNGVAGPAF"
     gene            17495..17736
                     /locus_tag="J1Y83_003008"
                     /pseudo
     CDS             17495..17736
                     /locus_tag="J1Y83_003008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_303550.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(17817..17975)
                     /locus_tag="J1Y83_003009"
     CDS             complement(17817..17975)
                     /locus_tag="J1Y83_003009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000499449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3927 domain-containing protein"
                     /protein_id="HBA7631138.1"
                     /translation="MFYKLCLLAVVILLLVMVMMDFTSRIMLVLTDGALVCGIVVLLW
                     PMMKEQNE"
BASE COUNT         4121 a         5470 c         4267 g         4320 t
ORIGIN      
        1 tcactgccgg tcagcatcgc cgccatattc tgtgcaatac catcaaaggt ctgcgtggct
       61 gcacttttaa cctgcgacat actgtccgtg gcgctctctt cccactcact ccagccggac
      121 ttcaggcctg ccatccagct cccgcgaagc tggtcttcag ccgcccaggt ctttttctgc
      181 tctgacatga cgttattcag cgccagagaa ttatcgccat actgttcctt caggcgctgt
      241 tccgtggctt cccacgctgc ctgccggtca gtcagccccc ggttttttgc atcaatggcg
      301 gcccgttttg cccgttgctg ctgtgcgaat ttatccgcct gctgtgccag cgcgttcagg
      361 tgctcctgat aggtgacctt gtcgccaagt gcagccagct ggcgtttgta ctccagcgtc
      421 tcatctttat gcgccagcag ggatttctcc tgtgcagaca gctggcgacg ttgcgccgcc
      481 tcctccagta ccgcgaactg actctccgcc ttccacaaat cccggcgctg ctggctgatt
      541 ttctcattcg ctccggcatg cttctccagc gtccggagtt ctgcctgaag cgtcagcagg
      601 gcggcatgag cactgtcttc ctgacgctct cctgcagaca ccttcacgcc ggactgcttc
      661 ggctttttca gtgtcgcttc atagtccttt ttcgccgccg ccatcagcgt gttgtaatct
      721 gcctgcagga tttttccgtc tttcagggcc ttattcagtt cttcctgacg ggcggtatat
      781 ttctccagcg gcgtcagcag acgctcatac cccttctgcg cctctccggt atacttcagc
      841 tgtgatgcgt cccgttcggc ccggtccctg gcggccagtt caccggcttt ttccatatcc
      901 gactgcagcg tggccgctgc cagccccagc cgggcatttt cccggtcatt ccatgcgccc
      961 tgaaggttgg cccggaaaga agaggtttta ccgcggcgct ggctccggct ctggtaccac
     1021 tgccattttt tatccgcctc atcaaatgcc ttctgcgcac tggcgagcat atccgctgag
     1081 gactcaggac gaccgatatc cagaatggca tcccacatcg atttgaatgc cttcccggtt
     1141 ttatccgccc aggtctccag tgtccccatg ttttctttca ggcgacgggt ctgctcatca
     1201 aagcctttcg tggcgatatc gttcgccgcc tgtaaggccc cggcctcatc accggaacgc
     1261 tgcagctgcg caacatacgc aatctgctct gccgtcacgt tacggaactg gcgcgccatc
     1321 gcaatcagcc ccgacgtaga gtcggtggtc agttttccga aagcctctgc aaccttgtcc
     1381 acctccacac cggatgcaga agcaaaacgc gcgacactct ggttgatggc atcaaactgt
     1441 tcaccaccac gcacaccggc attcaccagg gctgccagtg actcactcgc ctggttaaac
     1501 gtcagccctg cggcctgtcc ggctctggag agcgtcagca tgcgatcggc agtcagtccg
     1561 gactgattac cggaaagaac cagggtttta ttaaacgctg aaagcgtgga atccccctgg
     1621 taccaggcgt acgccagcgc acctgtcgcc accgccagcg aggtgacccc gaccatcggc
     1681 agggtgatcg caccggcaag ccccctgaac atggggatca tcccgccgaa ggagtccttc
     1741 acctgaccgc cctgttgcag caggatcagc cagggattct gaccaccggc aagctgcgtg
     1801 gcgatatccg taaactgtgc gggcagggtt cgcatggccg ctttatactg cccgacggaa
     1861 atcccggctt tttgtgcagc cagcgcctgg cggctcaggc cctgttcaac agcactggcg
     1921 gtttttctgg cgtcggtatc cagacctgaa aaatgacgcc ttacccggct catctgctca
     1981 tcgaaacgga ccgcatccag actaaggtca ataacaagat caccaaccgg ctgggacata
     2041 tctcacacct ccggaaatcc ccgctgaagc catcattaat gcggcatcat ccaccatgac
     2101 atccgccaca tccgcagacg ataaaatatc gcgccctccg tccccaccga accggacgcc
     2161 tccggcaagt cctgccgctt tctgcatcag cattttgtcc tcatccggcc tctccacctg
     2221 ctcttcctca tgccggggga caagcagact gaaatcagag ggatgcatat ccggatcgca
     2281 aaaaaacagg ctgagtacag cgtacgtcag cccggaaaaa tgcatatcca gctgggtatc
     2341 gtgaaaataa tgcatgcggt aaaaatgtcg ccagtcggca tattcggtgg atgtcatccc
     2401 ggcaagcatg gcgcgccagt caggcctccc catctcacgc gccagtctga gggcaaaatt
     2461 cagctcgccg tcgaagactt tcccgcagaa aaatcatcat cagtcagcgc gttatttttc
     2521 gccacttcgg taatatccgt atccacatga acaggcccgc tcatcccgga cagacgcaac
     2581 accacatctt ccgcccgggc aatggcatca gcaggccagg tggtcaggac ttcctgctca
     2641 atctgcatca cagcctcatt cattgacggt gaccccgttt tctgcggatg gttatgccac
     2701 agggacatcg ccaccagaaa cgcgccggtt ctgacgagat cttccacgct tacctgcagg
     2761 ttgccgctgg attctgcctg ttctgcacgc cgtttcagga gggcaagatg ctcaatacgc
     2821 tgcagcgcag acaattcgga aagcgtgaca gacacaccgt tatattcaaa ttgttctgtt
     2881 ttcagaaaca tgtatgacct ccgtttaccc tgcagcgcct gcttcagtaa cggtgacttc
     2941 agctaccgtg gcaaactgac cattaccgga aatcacgggg atactcactt ttccagcctt
     3001 aacccccgtc acagtgatca ccatatcttt cacagcaatg gttcccgttg acggatcggc
     3061 ggaaaccgct ctgaacgtct tgtcggttgc actttccggc tcaaaagaaa cagtcagggt
     3121 ggttgttttt cctttttcca cggtaccgga tgtcggtgtc accttaattg cagtggccgg
     3181 cgtaatttcg ctgcgttctt ccgccacgga aggtttaccc acgttggtca ctttcaccgt
     3241 acgggtgatc acttccttcg ccgttacggc cttaccgata ctgctgaccc agccacggaa
     3301 cacatccacc gtgccgttcg ggaaacggat tttataggcc cgcacatccc cgctttcaaa
     3361 ccagcctata agccctttct gaccttcttc tcccggtttc caggccagcg taaaactggt
     3421 atctcctgca gacttctgcc cctgcccggt cgcgctccag tccgcgtctt catcatccag
     3481 gtagttatca tcgtaggatt ctgccgtcat ctcgcccggc gtcagatcct tcaccttagc
     3541 cagtcgctgc cagtcatcat ctgacaacgg gtttgcataa gcatcaccct tgccggtgta
     3601 aacccacaga gtggtaccgg caccttttac cggcgccagg ggatttggtg ttggcatatc
     3661 gtcctcacat ctcgtatgta atggaataag tcaaatctgc agagctccat aacgccatat
     3721 cgtcatcacg acgatagtca tagccctgct gaaccatcgt ggtaatcatc cctgccagtg
     3781 cagggatcgc ggacatcgcc ggataaatcc gggactccat ccacgaatcc agctctgaat
     3841 ccggcacctg agcaggcagg aaaacttcaa tatgcagtgt ggcccgccag gtatccgcat
     3901 ccagctcttc accggtatac tctgcatccg tcagataaac cgcgaccgcg ggaaaatcct
     3961 cttcgtcaaa aacaacgggg cgaccatcaa acagcgtcgc cccgtgttca tgcagctcca
     4021 gtgcatccag cactgcagca cggatatcag tatgtttcat cgttttatcg caatcctcag
     4081 ttgttgtttc agcgcatatg ccagttctcc gggcaggcgt tcacgccgga tacggtcaac
     4141 gttttcatca aacgcctgtt tcagtggggc cgccatcggg attttcacca catcaatggg
     4201 gtaacggttt ttcccggcca cacgctgcat gacatgccag cgaccatttt ttaatcgctg
     4261 aataaatgcc cgctgataac gatgctgacc ggctttgagt atgctgtccg gacgacgccc
     4321 cggcatcctg atccccagct taatcaccgg gagatcaccg cggttaacga taattctggc
     4381 attcggattt ctgaccgtgg cccgtttcag tctggaccgt tcctttacca gtttccggct
     4441 caccctggtc tcccgggcaa cctgtgacga agactgatta atcgccgttg tggccacgcg
     4501 gttaatggcc attgctgaag ccgccggaat ggcgttttta cgaacccggc tcagattttc
     4561 aatcgcctga tcaagccctt ttatcgccat aattcaccct gcgtttatcg tcgccggtta
     4621 actgccggtg gttgcccacg gttgagccag agataacaac tgcccccgtc atccggagaa
     4681 acacgatcca cccagaatgt ctcaccatta atggtcagcg tgtcaccacg ccgcacagca
     4741 cgaaccgtat ccgtccgtac aaataatgac gggcagcttc cttcaatacg gaccccgcta
     4801 ccggcaaacc ccagcgactc cggatcgtca aaaaccccct gaacttcgct gccacactgt
     4861 gcccccgagg tgaactgcgc acagagcccc atcacttcaa caatcgtgct gtccaccccg
     4921 gcaagggctg catcaaaggc attctgaaaa tcacgcatgc tcagccgttc cgtgctgtat
     4981 catggctgtc gccagtggtg atggcaccag aacacgcatg ccccggtacg tcagttcaac
     5041 gggacggcct gtctccgggc aataccccat cacatgcagg cattgccgca cacggaccgc
     5101 tttaacatca tccgtagcat cagtgttgtg caactgctca ccatcgtctg tgtgattttg
     5161 ctcaggcccg ctctcatcac caggcataat gccctcccgg gaagcagcaa gctcctcttc
     5221 ccactcagac acacgttgcg caatatccgc agcactcccc gacatatccg cctcgcgccc
     5281 cagcaggcca gccagttgac gaagacgttt ctgattttct tcttttgttg ccatatcagc
     5341 cccctgtgaa aaaagacacg ggggcatttc gcccccgctc acggattatt tcacctgcac
     5401 caccacaaac tcatccggat ccggcagcac catcagcggt gcggactgcg tcatggtaaa
     5461 ttcacgggcc ggatcgccca cggtcagcca gtgtttcggg taacgggaag aagccaccac
     5521 accttcggac aacgcctgcg catcctgaat ggcaccgtaa caacggatcc catctgcagc
     5581 cgtatttccc aggaccagcg tgccctccgg cagataacgt ttttcggtac cgtcctctgc
     5641 cacataagac gttttcgcca ccacaatggc cagatcgccg taataccctt tgaaagacac
     5701 caccgccccc aggtctttca ctgccgtttc gagttgtgaa tttgagccgc gacgggtatc
     5761 cagtttttct cggaacagct taaaaccatt cagcagacgc cagacagtac cgtccataat
     5821 ggcaatattc acaagaccgc tggcctgatc gcagtagagg tcaatatcat gcgtcggatc
     5881 aaacgtatca cggtcctgct cagaccattt tttaccgtcg gcctgctcaa tgttatttcc
     5941 ttcagagcgt ccaaaatcca cctcgacagt atcaaactgc tccccttcca tggtgtattt
     6001 gccatacagc acggcattca ccgcctgcat ttcttccacc tggacaatgg catgctcttc
     6061 ctgtttgagg ttatcggtga tgatacgcag acgacggtag gccgggtcgt tcagttgagc
     6121 cggatcttca ccgggaagac gctcaaccgc ctgctggtaa ttaaattcgt gtttcggctt
     6181 gacgtagccc ggacgtaaca cgcgggtttc accaccgcga tgacgcagca cttttccttc
     6241 aacaaccggg gagacatagg ccgccaccgg cgtttttccg gtaattttgt ccagcatcac
     6301 ctcttcggta tggaaattca ccgtacggcg gaaaaacagc tccagaaaca gcgcacgaaa
     6361 tttcactttt tgttcggtat aaccgagtaa ctggcgggtc gtaaacaatc ccataaatca
     6421 gttcctttca ttcagaaatc agtcaggcca ccgcggtggc ctgataacgt gttacggcag
     6481 cgccgcgtga ctcagggcac tgccggcaaa ggcatttgcc tttttgtgtt catccacact
     6541 gtcaggccag cggattgcct ccgtcgcaaa ggtccccgac ttgtaatacg tcagtaccgt
     6601 ctctgtgcct tcaagcggca gtaccagtat gccaaccgca ctaccggctt tctgtccgtc
     6661 ccagaccacc agtttcccgg tagcttcatc cagcatcagg ggcgtcagtg ccggtgttgc
     6721 cgaggaaatc ccgctgctgc ctgtggcggt atgagccgga tcattaccgg caaaaatacg
     6781 tacttccgca cgctgttcag tgatggtttt cgttaccata ttgtaaaaac ctcctgttga
     6841 tggtcagcac tgacttcatg gcatagccat gagcattttc acgtccgcat caccgtctgc
     6901 tgacgtctgt ggcacgccac cctgtaccgc tgccggtgaa tggttcgcca tgatgcgttc
     6961 aaacagggcg gttgtggatg cagagaccgg ttctgcctta cctgatcccg cagccagcac
     7021 agcccgggcg ctctccacag tcattcccgg gcaggcagcc agctgttcag cctgcgcctc
     7081 agcccctttt gcctcatcca gtgccatgat ctgatcacgg agtgagggtc cggcatccgc
     7141 ctgcggtgaa gcagccagga tcgggcgggc tttttccacc gtcatctccg gcatcgccgc
     7201 cagcgttgcc gccagttgtt cacgaccgtt cgcttcttca cacgccataa tgcgatcggc
     7261 ttcactctgc gtggatgcca ccggctgctg cggtgccgcc gcggccagaa tcgcccgggc
     7321 ctgttcaacg ctcatgccct gttgtcctgc cagcatcgtg gcaagctgtt cacgtccttt
     7381 cgcttcctgg catgtcagga tccccataac tcgctggttc tcctgtgcgg cggcttccgt
     7441 tgcagttaat tgcggcatag tgcctcctct gacattactg ttcagcgccg tggccatcac
     7501 actgatggca tccgacgcat tgactaattc atccgccagc ccggcatcaa tgccggactg
     7561 accttcaaaa acggcggcct ctgttcccgt gacggcatca acagacaggc cggtaaacat
     7621 cgccactttt tcggcaaaca tccggcgcgc cgcatcaatc cgctgctgca tgtcctggcg
     7681 aacctctgcc ggcaacgctt caaactgatt gccatccacc ttgtgcgccc ctgagtaaat
     7741 cagcgtgata tccacaccgg cctgcgccag atgaccggca tagctgacat ggctcatcat
     7801 cacgccaatg gagccgatac gggatgtctg ggtaaccagc cgtcgggagc aggccgacgc
     7861 cagcagcatg gctgcagaac aggccgtgtc attgcacagt gcccagaccg gcttctgctg
     7921 acggaggcgg taaatcatgt cagcgcagtc aaacgcgccg gcggcctgcc cgcccggact
     7981 gtcaatatcc agcagtacgc cctgcacctg gctatccgcc attgcctgct gaagacaggc
     8041 gacaatgccg tcatagcctg tcattccgga aaatggccgc atacccccca gccggtgcac
     8101 cagcgtgccg gtcactggca gtacagcaat accgttcacc acccggtaaa cacgggccgg
     8161 tcgtttacct ccggccatgt actcgtccgt ttcagccagc attccgggag catcaagctg
     8221 tacctgctgc tgtggtaccg aaagacttgc tgcccccatc tcgcgcccga gcgcgcaaaa
     8281 gaaaacccgc gcataggcgg gctccagaag cagcggttca ttgaatgctg cggcaataat
     8341 gtgtgaaaga ttacgtctca cgtggtgttg tctcctcttc cggcctgcga ctctccgcta
     8401 tctgctgctg atacgcctgc gctatccaca ccggacgtga gagtccggct ttttgccgct
     8461 cagcagattc cctgacctgc tggcggaaaa tgtcctgata atcctcgccc atcagcgcca
     8521 gctctttctc atacgtgctc agtccggcct caatgcgcat cactgattcc tggacttcct
     8581 tgagcccgtc aatggccatt cttccggcac caatccactc tgcccgtgac caggctgatc
     8641 gcgcctgata aaaatcaaaa cgtgcccgtg gcggacgaat aatcccccga agaagtgcct
     8701 cttccagcca gcaggaaaac atctgcgtgg ccagccgggc cgcaataaat tttcgccgcc
     8761 ccataaaata gcgccacgac tcattggcgg aggcgcgggc acttgaataa ctgaccttcg
     8821 agtaatcacg ggacaactgt tcgtaggaaa cgccaagacc ggcggcgata taccgcagca
     8881 gcgcctgttc aagcgccgaa aatccattgt ctgaatcctg cgcagtctgc agtttcagat
     8941 catccccggg gaaaaggtgc ggaattctga caccgcccag cgtcacgtta ttcgtgtcat
     9001 accagctgga gaacttctcc agaatattaa taagcggatt atccttctgc ccctgcggcg
     9061 caccggcgat atattcaaag gccttttcgg tatcaagttc actttcaatt gtcgctgcat
     9121 acatggcttt cacaatggcc gactgaagct gtgttgcctg cagggaatcg agcatcttca
     9181 gccgttccat gacgctgtaa aactgattag ccccacgggt ctgcccgtcc tccaccggct
     9241 cgaaaatatg cagcatggcc ggacgcccgg tgggaagttc acgcgggatc cgttcccatc
     9301 gaccactacc agagaacgga aaatcatcct cacagatatg gtacgcaacg gcacggccat
     9361 atcgatcgac ctccacaccg gcccgtagaa agcggttccc cataccgtgt ccaggcgtgt
     9421 ccacccgttt cggactcacg gctttaaaac gcgtacggaa taactgcgtg gtttccgtat
     9481 cccagaccgg ctgcacaaag atttcgccgt taaagacatg aacgcccaca ccttcacgga
     9541 taaattccgt aaacgtgcgt ttcccttcca cgtcgatctc gccagacatc ccttctgcgt
     9601 attccgacca ggccgcctcc acctcatcga caaaactttt tgccgcggtc tcccgcatcc
     9661 ccagccagcg ccagttcgga cggtagctga tcagaaacat atgcccgaca atgtgatcct
     9721 tatgcagagc caccgcattg gccgctattc cgttattgcg caccagatca tctgcccggg
     9781 cattccccag acgcaacgcg ggcagcaggg ccgcatcggc actctgcgcc ggtggcaacc
     9841 actcagccat ttgcccgcca aatcctgcac cgcccccgtt gtagctgaga ctctcacgaa
     9901 gcggaacgcc gttcacatca atcaggacag gcgttcgttt cataacctca ctcccagcgg
     9961 acgacggcga cgccgggttg tccccagtac cgactccgca tcattgatcg cccggttaag
    10021 ctcatccaga gaagccgccg tatattcaat tctgcgacca tctttctgga cagacaccac
    10081 ccgtttaccg gttaataaat caaggcgcgc ctgacgcagc gcctgcagtt cagcgactgt
    10141 aaccattcac tcctccggac agcttcgctg ccagttcttt aagggttggc cgggtcgtct
    10201 cttcttcccg ggattttgcc agtacagcca gatcaagctg ccagcgttgc acggacacac
    10261 gtaatgccgc gtaggcatac accaggcagt ccagcgcttc gttacgccgc tttttgttat
    10321 cccacagcag acgcatcttt cctttttccc acttctccac aagctcttcc gcgaccagtt
    10381 gctgcgcctc tgtctgcgaa aaaatctccg gatcatcagg aaaacggatg gcatacgacg
    10441 tggcttcatc cacaggcgtg ggatcggctt tcatacgggc atagagaatt tcttttgcgg
    10501 tgtccgttcc cacttcacac agatacacgc cccgctgatt gcgggttttt ggcatggtga
    10561 tcaccggctt gccatagaca gatgcgcctt ttaccggcag cacccggaaa acaccgtgtt
    10621 tttttgacct ctgataaacg atttcgccat cgatcccccc ggtgtcccag cagacacggg
    10681 aaatagtcat ttcggttccg tctgcatggc ggtatttttt gttgatcgcc gcatccacac
    10741 gtaacagcgt ctcttcctca tcgggacgcc ccataatgat gattttatcc accagaaagg
    10801 cttcctctcc cggagcccat ccccagacat acatctcaaa acggtttcgc tgcgagtcaa
    10861 tgcccgccgt cagataaacc acccgggcag gcaccgccgc cgtgtaacgc acaaccttat
    10921 ccatcagcac ctggtgatcg agtttttcgc ccacggcctc ttcccaggtc tcgcccagcg
    10981 tggtgttcac aaaggttttc aggccgttgg gatctttcag ggcatccagc cagtcataga
    11041 caatctgtac ccaggtggtg aacggactgt acgccgtcca gatatggaaa gtgatggagc
    11101 gcggcggcgg aatttcatca ccccgggcgc tgaaaaacat caggccgtca cgggtcaaca
    11161 tgcccgtgtt ttcacagatc caccgcccgt tactctggtc aagctcagac tgatggatca
    11221 cacagccatg atgctcacaa aggtagaaaa cactttctgg cttattcttc tcccacttaa
    11281 gaccgaaagg cgaggcatca tcaccaaatt tcagatactg ctcctcccca cagtgcggac
    11341 agggcacata aaaacgcatg aaatgtgccg actcgttagc ggctttttcg atctggcagg
    11401 agcctttgat tttaggcgtc gagccgcgaa tggattttgg ccatacagag ccctcaatac
    11461 gtttatcccc cagcagggtt ggcgacccct ctttttcaac atccggctcg aacgaggaaa
    11521 gttcgtcata gcagaccacg tccacggatt tttcacggta gtttttggcg gcagcgccgc
    11581 ccaggcacca gaaaccgacg cccgatgaaa agcgtttcag cgtgagagta ttgtcacgat
    11641 gtttacgacc cagccatggg gaaaggtctt tcaggcatgg cacgttccga atcgtcgcct
    11701 ccacgtgaga cttcataaaa tcttcagcgg cagaatccgt gggctgaaaa agcagactgt
    11761 ttcgggattt atgctcaata aaatacccga ccacccccag caacatcttt gtatagccaa
    11821 cacgggcaga tttaatcaga ttaacagtgc ggatctgatc attccccatg ctgttcatga
    11881 tggcgatctg gaatggcagc gttttccatt cgccctcacc atatgaagat tctttaggca
    11941 gataataatt ttgatcagcc cattcaactg gcgtcaccgg caatgccctt atcaggggct
    12001 gtaatgcagt tgtgacagcg ctcatcatat tattcagttg ttgctctgat atattcatca
    12061 agtaaatccg gtaatttatc ccctgcccgc gcacactgat ttgccccctt agcaataagg
    12121 gttttcagat ggtcaagatg gcgcggtgtt aaatcaggaa actgtcgctg catggataaa
    12181 gggatggaat caagcgtact ggataacgcc attgccagct tgctgagggc aaaaatacag
    12241 aacccggtgt caatcagttt tccttttgac acctcatttt ttaactgctg tgtaacagcc
    12301 tgttctgctg tcagttccca tctggcaata agcaatttct cttcatagtc ttcttcgcta
    12361 tcgccatcag gcacatcgtt tttacttctt ctcagatacg atatgtaaaa atcgcgccag
    12421 gcatccagat ccagttgccc tcgcttattc gagaccgggg cacccggtaa tttctgcaat
    12481 ctgcgaagct ggcgatcggt cagactcaaa tgcctggcaa cttcagtctg cgtagccact
    12541 cctcacctcg caaaaactct tacttcacaa tcagcacaaa accggtcatg tccggtttac
    12601 atgtccgttt tttgcacatg tccggttcac ggacagcctg tttttatatt tttcatatag
    12661 ttaacttgca gagaaaccgg acatggatcc cggaaaattt tcataaatag cgaaaacccg
    12721 cgaggtcgcc gccccgtaac ctgtcggatc gccggaaagg acccgcaaaa tgataataat
    12781 tatcatctac atgtcacaac gtgcatctac gccatcaaac cacgtcaaat aatcaattat
    12841 gacgcaggta tcgtattaat tgatctgcgt caacttaacg taaaaacaac ttcagacaat
    12901 acaaatcagc gacactgaat acggggcaac ctcatgtcaa cgaagaacag aacccgcaga
    12961 acaacaaccc gcaacatccg ctttcctaac caaatgattg aacaaattaa catcgctctt
    13021 gagcaaaaag ggtctgggaa tttctcagcc tgggtcattg aagcctgccg tcggagacta
    13081 acgtcagaaa agagagcata tacatcaatc caaagtgatg atggatgaac atcccggttt
    13141 cttccaccat cgcaccggaa aagcgactat gagggtaacc ctgcgtctgt cagcacagta
    13201 aaacccggtg tgcatcgttt ttgattattc ccgcacactc acgcagaagg aattccccgt
    13261 cgggctacgg tcatggttaa tgcgggaata cggcgacgat acagcgcata atgtgtcagg
    13321 cttgaatacc tttatcgaat cccggtaata aaaactgtcc ctgtctctcc agacgttcca
    13381 gctttgcaag caattgaggt tttttcgttc tcccccagcg attgagcaga cggcctgaca
    13441 tgctggcgac atccttctct ttcatgtact ccagcattac ggcgttacgt tctgcttcat
    13501 aactttcgct gtacttacgg agttctgctg acatccagtt aaacgcattg atataggctt
    13561 ctttaacagc atcggctttt gccccgttaa atcccataac cagcataacg aagccactaa
    13621 agtccatgcg gtagtaaatc tgtttcttgt caaaaatacc taagtcattg attttctcga
    13681 cggcccaaaa atgggttgtc gagaattcct cagaacatct caaacattta atagccctaa
    13741 tcacatgctg atggcgttta ccaaatgccc tggcaatctg gaaggtgtca gttaccggtt
    13801 gaccttctgc tgcggtaact aactggcgaa agtcgaagtc atgattcgca attaattcat
    13861 tcatggcgtt gcctgcttct ttgaaatgaa cctttgccgc ataggaaacc agcccaccga
    13921 ggctcgccag cactaactgg tatcctcaaa ggcccattcc aaagggtcag gttcggtgtt
    13981 tattgtgcgc tgcggtgcgc ggtgaaatac cggtacaaaa atgccccgca tctgcgaggc
    14041 attttcctga aagtcacttg ttaaatttca gtgaaattaa aattatttta agcactgcgt
    14101 cctgatgtat tcctgcaggt agttaacctg cgcggttatc ttgtcgattc cacctcggag
    14161 acggtaataa ttgagttcag catctgctgt aagtcctggg ctttctccat cgcccatgct
    14221 gctggctccg gtcgttgact ttgcacaggt ggcggagact tgcaggcgct tacgcccagc
    14281 agaaacatca gcacggagac tttcgatagt cgcattagca tcagcaagct cctttgtgta
    14341 tctggcgtca agttctgcta catcacgttg ccgcttctgc atatcagcga tgatggatgt
    14401 ggctttatcg cgctgctctt tgtaggcgat ggcgttatca cggtaaagat taacagccca
    14461 tgacaggcag gcgataatgc agataaccag agcggagata ataacggtta ccctgctcat
    14521 tgttgccccc acaaacagac ttcacgctca atctcgcggc gagtcatcag ccctttccat
    14581 tgcttaccgc cagcgtatgt ccagcgccgt agctgatcac atgcgctttt gatatcgccc
    14641 tggtttattt tgcgaagaag cgtcgatgtt ctgaaattgc cagcgcccac gttgtaaacg
    14701 aacgagtaaa gagcgccgcg cgttgtttcc ggtatatcga cgttgatgta cgggttaatt
    14761 tgtctggcga ccgtggcaag gtctttattc aggagggctt tgcattctgc ttcggtatac
    14821 gttttaccgg gcatgatgtc ttttccggtg tgtccgtgac atacagtcca tacgccaacg
    14881 atatctttgt atggtatgta gctgacacct tccagaccat cgttaccacc tggaccagtg
    14941 atgagcacag acgctatggc aacagcccca ccaccaatag cagctgcaac agccttgcgt
    15001 aatgacggcg acattattca cctctcgcag ccttacgctt atcttcttta atcttgaaat
    15061 aaagatttgt cagatacgtc agcaggccaa acagcagact tcccagcaca cctattgcca
    15121 cccactggga cggagagact ttgtccagca gctgcagtaa ccagtatccc gtccccaccg
    15181 ctgacgtggt gtatgacaca cctgttgtga ttttttccat ctgatgtatg tctccgtcac
    15241 cgccgacaga aaatgaaagt aaagaaaaac aaaaaagccg ccagtgtcac ccactgacgg
    15301 ccaacgccgg gagccgtgat tatggcattc aggctctgct aaaaatgcca gataacattc
    15361 cggcctcccc tgattcaggt tataaatgac acaatatctt gacaacatcc gtcactgtct
    15421 gtcagaaaat gtactgccat atagaagcaa catgtgaagt acatctatcc ttttgagcca
    15481 gcacctctcc accgaaagtc agtgctggct gtttttttcc ttaataaggc atctgtaact
    15541 gaaacaatcc gcatattgat aatatattga caggcatcat tgctgtctgt gaaaaataag
    15601 tctctacaaa catataaggc cttttagcca gcgtcttctt tttcaggtca gtcgctggct
    15661 ttttttatta tgctgccggt gcatttatct ccagcatcag actttctatc tcaacgccat
    15721 acgctgcatt ttttgtaaca tccgtcagcg tcagcgcatt cagtcccagt gtcagactgt
    15781 cttttataac ctagaatgcc gggccagcca ctccattcag tttcggagta accgtggcac
    15841 tgccggcggt gaacaccagc tccagcgtct gccagtcgtt accgtaatcg ccgaactccc
    15901 ccagcttcgt gtttccggct ttcctgtgat gcatcagatt cactctgccg tcagtggtct
    15961 gagtgaagta cgacatcagg aacggattac cggtacccgt catcgccaca ccatcaggaa
    16021 cgggagcatc cgtatacaga taaatcccca gcccgaactg attgttggtc agtgcgcctg
    16081 acaggcggaa cttacaggtc agtctgccgc cctgtgtcag cagggtaatt gcgtcatcca
    16141 ccggatgcgt cagggaccag gttttattgc tctgcttggt gatcttaaat acaccatctg
    16201 acaactgaat tccgccatcc ttaatgctcc agccctgcgc agcagcctct ccggctgccg
    16261 gcagcaggga gattgtgcga acggacgtat ctgcagacgg acccgatggc gtgttgccgc
    16321 cgggcgaggg tttgatttcc ggtgccttac cactgatgaa ggctgaggtg cgcccggctg
    16381 cgttcagaat agcggttgcc agacgatccg gaataatgct cctgcgcgcc catgaactga
    16441 aatgtgtcgg gcggtttgat gatacctggt ttccattcgt tctcgatgcc gcaccgtaat
    16501 atcctgatgc cggaatatcc ggatcttctg ccggcgcgtt agtggcggta ttgacgccgt
    16561 taccatctgt catgaagggc acaaaataaa cgccctcact ctccctgttt ttatacccgc
    16621 cgtacacggt gtcgtactgg gtagcgtatg tatttttcca gtaatacgtc gtgtcaccac
    16681 aaacccacgg cacatctgca gcactgccac catggcactg cgcgttaaac acggagaggt
    16741 cagcacgaaa ctgtgtcagc atggctgtaa acagcgcagg ttgctgtgcg tgggtggcgg
    16801 cgctcatgtc aaactcaccc tgcatccagc agacggccag cagaacgttt ttgggatttt
    16861 tctgcaatgc cgcttttgtg cgggaaatca gatcctgata taacggcttg cccacccccc
    16921 agcgtgccga atcctgactg gcccccgtgg actcgctgaa tgtcccctcc gcgccctggg
    16981 taaatgccga accaccacga cagcatggta ccagcaggat ccccgcgtta ttcgggatat
    17041 acgggagcag ttttttggca atatgtaaac cctgaccgac acagccgtac tgccctttgc
    17101 tcaggtcagc cctcggatga ttcagcgtac tcatatcctg cacatcatgc agacagtggt
    17161 cagccggaat aatatcgtta tatctgcagg cagccccgcc cggcgtcact gtactgcggc
    17221 gcgccagctg tttaatgcgc ggatccggag catcgtatga atccggcagc ggaagccctt
    17281 caccgtaagc catggcattg gactgcccgg ccagtacgat gacgtagtac caatccggct
    17341 cagatgaagg gccgacctgt ggctctcctt caatagccac cgcctgcatc agtgtgtacg
    17401 gcgtaatggc aaccggtccg ccgtatggct gccagccctc tttcagtttg tgtgtcagct
    17461 tttccgcaag gtctgacggc gacgccgccc tgacaacatc gtaatgttta atcgacatcg
    17521 aatttctccc gtgtagagga acagagttaa aaagccggaa gcggaatcaa atcacaggat
    17581 gaccatctgc cagtggcagg tcataaaaaa aaggctgcgc aatgcgcagc cagaacacac
    17641 aaggaaaatg ataaaaggaa taacactagt gatgtacgca tggcgcctcc cgctaagttc
    17701 tgcaatgatc aaacagaact cgctacgtgc ccttaaaact cgatcattta gcccctccaa
    17761 ggaggattca ccatgcggtt gattttttaa taaacagtaa acaaaaaagt caagaattat
    17821 tcattctgtt ctttcatcat cggccacagc aataccacaa tgccgcagac cagagcgcca
    17881 tcagtcagta ccaacattat cctgctggtg aaatccatca tcaccatcac taaaagcagg
    17941 atcacaacag caagcagaca cagtttataa aacaatgttc agaaaacgca ttcagcatgc
    18001 ctaaggttct attcctacga atagccaact tgcaacttaa aatattattt atgcagccaa
    18061 ttaaattctg gtccttacaa tatcaacctg aagattctta tcttgtgctg attgataaat
    18121 gacaaacctt ttactacctg cattgaaaga agtagacaaa accagacaat tatcataa
//
LOCUS       DADSYM010000103        18126 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2415
            SAMN08039579-rid11723173.denovo.103, whole genome shotgun sequence.
ACCESSION   DADSYM010000103 DADSYM010000000
VERSION     DADSYM010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039579
            Sequence Read Archive: SRR6362533
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18126)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18126)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980162.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 03:39:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,484
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 4,347
            CDSs (with protein)               :: 4,347
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 43 of 85
            Pseudo Genes (incomplete)         :: 37 of 85
            Pseudo Genes (internal stop)      :: 19 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18126
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039579-rid11723173.denovo.103"
                     /strain="LGE2415"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8302 N 95.9628 W"
                     /collection_date="2015-08-24"
     gene            complement(440..1030)
                     /locus_tag="J1Y83_003010"
     CDS             complement(440..1030)
                     /locus_tag="J1Y83_003010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001260226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA7631139.1"
                     /translation="MRTPSRYIFRLPSHEINPFRATLLLILLICAVLAGVSWLILSFV
                     RTGNTFIFWLILFIGYLIAIAKQEKIKLIEKRQIMADKRQGLSICQFARQFSPHTVDT
                     WVIRAVWNTLQGNGYIDYPLPLKASDKLDDDLDLVNDADELEELVEDIAARCGRDLRG
                     IEDNPFLPITTVGSLVSVLNAQPMTQERRSLLFTRS"
     gene            complement(1080..2276)
                     /gene="hemY"
                     /locus_tag="J1Y83_003011"
     CDS             complement(1080..2276)
                     /gene="hemY"
                     /locus_tag="J1Y83_003011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protoheme IX biogenesis protein HemY"
                     /protein_id="HBA7631140.1"
                     /translation="MLKVLLLFVLLIAGIVVGPMIAGHQGYVLIQTDNYNIETSVTGL
                     AIILILAMVVLFAIEWLLRRIFRTGAHTRGWFVGRKRRRARKQTEQALLKLAEGDYQQ
                     VEKLMAKNADHAEQPVVNYLLAAEAAQQRGDEARANQHLERAAELAGNDTIPVEITRV
                     RLQLARNENHAARHGVDKLLEVTPRHPEVLRLAEQAYIRTGAWSSLLDIIPSMAKAHV
                     GDEEHRAMLEQQAWIGLMDQARADNGSEGLRNWWKNQSRKTRHQVALQVAMAEHLIEC
                     DDHDTAQQIIIDGLKRQYDDRLLLPIPRLKTNNPEQLEKVLRQQIKNVGDRPLLWSTL
                     GQSLMKHGEWQEASLAFRAALKQRPDAYDYAWLADALDRLHKPEEAAAMRRDGLMLTL
                     QNNPPQ"
     gene            complement(2279..3472)
                     /gene="hemX"
                     /locus_tag="J1Y83_003012"
     CDS             complement(2279..3472)
                     /gene="hemX"
                     /locus_tag="J1Y83_003012"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HBA7631141.1"
                     /translation="MTEQEKTSAVVEETREAVDTTSQPVATEKKSKNNTALILSAVAI
                     AIALAAGVGLYGWGKQQAVNQTATSDALANQLTALQKAQESQKAELEGIIKQQAVQLE
                     QANRQQETLAKQLDEVQQKVATISGSDAKTWLLAQADFLVKLAGRKLWSDQDVTTAAA
                     LLKSADASLADMNDPSLITVRRAITDDIASLSAVSQVDYDGIILKLNQLSNQVDNLRL
                     ADNDSDGSPMDSDGEELSSSISEWRINLQKSWQNFMDNFITIRRRDDTAVPLLAPNQD
                     IYLRENIRSRLLVAAQAVPRHQEETYRQALENVSTWVRAYYDTDDATTKAFLDEVDQL
                     SQQNISMDLPETLQSQAMLEKLMQTRVRNLLAQPAAGTTEAKPAPAPAPAPQADAPAA
                     APQGE"
     gene            complement(3494..4234)
                     /gene="hemD"
                     /locus_tag="J1Y83_003013"
     CDS             complement(3494..4234)
                     /gene="hemD"
                     /locus_tag="J1Y83_003013"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HBA7631142.1"
                     /translation="MSILVTRPSPAGEELVSRLRTLGQVAWHFPLIEFSPGRQLPQLA
                     DQLAALGESDLLFALSQHAVAFAQSQLHQQDRKWPRLPDYFAIGRTTALALHTVSGQK
                     ILYPQDREISEVLLQLPELQNIAGKRALILRGNGGRELIRDSLTARGAEVTFCECYQR
                     CAIHYDGAEEAMRWQSREVTTVVVTSGEMLQQLWSLIPQWYREHWLLRCRLLVVSERL
                     AKLARELGWQDIKVADNADNDALLRALQ"
     gene            complement(4231..5172)
                     /gene="hemC"
                     /locus_tag="J1Y83_003014"
     CDS             complement(4231..5172)
                     /gene="hemC"
                     /locus_tag="J1Y83_003014"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HBA7631143.1"
                     /translation="MLDNVLRIATRQSPLALWQAHYVKDKLMASHPGLVVELVPMVTR
                     GDVILDTPLAKVGGKGLFVKELEVALLENRADIAVHSMKDVPVEFPQGLGLVTICERE
                     DPRDAFVSNTYDSLDALPAGSIVGTSSLRRQCQLAERRPDLIIRSLRGNVGTRLSKLD
                     NGEYDAIILAVAGLKRLGLESRIRAALPPEISLPAVGQGAVGIECRLDDARTRELLAA
                     LNHHETALRVTAERAMNTRLEGGCQVPIGSYAELIDGEIWLRALVGAPDGSQIIRGER
                     RGAPQDAEQMGISLAEELLNNGAREILAEVYNGDAPA"
     gene            5559..8105
                     /gene="cyaA"
                     /locus_tag="J1Y83_003015"
     CDS             5559..8105
                     /gene="cyaA"
                     /locus_tag="J1Y83_003015"
                     /EC_number="4.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate cyclase"
                     /protein_id="HBA7631144.1"
                     /translation="MYLYIETLKQRLDAINQLRVDRALAAMGPAFQQVYSLLPTLLHY
                     HHPLMPGYLDGNVPKGICLYTPDETQRHYLNELELYRGMSVQDPPKGELPITGVYTMG
                     STSSVGQSCSSDLDIWVCHQSWLDSEERQLLQRKCSLLESWAASLGVEVSFFLIDENR
                     FRHNESGSLGGEDCGSTQHILLLDEFYRTAVRLAGKRILWNMVPCDEEEHYDDYVMTL
                     YAQGVLTPNEWLDLGGLSSLSAEEYFGASLWQLYKSIDSPYKAVLKTLLLEAYSWEYP
                     NPRLLAKDIKQRLHDGEIVSFGLDPYCMMLERVTEYLTAIEDFTRLDLVRRCFYLKVC
                     EKLSRERACVGWRREVLSQLVKEWEWDDSRLAMLDNRANWKIDQVREAHNELLDAMMQ
                     SYRNLIRFARRNNLSVSASPQDIGVLTRKLYAAFEALPGKVTLVNPQISPDLSEPNLT
                     FIYVPPGRANRSGWYLYNRAPTIESIISHQPLEYNRYLNKLVAWAWFNGLLTSRTRLY
                     IKGNGIVDLPKLQEMVADVSHHFPLRLPAPTPKALYSPCEIRHLAIIVNLEYDPTAAF
                     RNQVVHFDFRKLDVFSFGENQNCLVGSVDLLYRNSWNEVRTLHFNGEQSMIEALKTIL
                     GKMHQDAAPPDSVEVFCYSQHLRGLIRTRVQQLVSECIELRLSSTRQETGRFKALRVS
                     GQTWGLFFERLNVSVQKLENAIEFYGAISHNKLHGLSVQVETNHVKLPAVVDGFASEG
                     IIQFFFEETQDENGFNIYILDESNRVEVYHHCEGSKEELVRDVSRFYSSSHDRFTYGS
                     SFINFNLPQFYQIVKVDGREQVIPFRTKSIGNMPPANQDHDTPLLQQYFS"
     gene            complement(8145..8465)
                     /gene="cyaY"
                     /locus_tag="J1Y83_003016"
     CDS             complement(8145..8465)
                     /gene="cyaY"
                     /locus_tag="J1Y83_003016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000999950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron donor protein CyaY"
                     /protein_id="HBA7631145.1"
                     /translation="MNDSEFHRLADQLWLTIEEHLDDWDGDSDIDCEINGGVLTITFE
                     NGSKIIINRQEPLHQVWLATKQGGYHFDMKGDEWICDRSGETFWDLLEQAATQQAGET
                     VSFR"
     gene            complement(8513..8776)
                     /locus_tag="J1Y83_003017"
     CDS             complement(8513..8776)
                     /locus_tag="J1Y83_003017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000580503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA7631146.1"
                     /translation="MIGTSRQDGFMHYRNVVCLLSCSLFLSSAWGCRLDEPEHNIYQK
                     QGKGVVYLRPYEKTNLSLYRDIIIPVTDESCRNAAADGQTLKP"
     gene            8935..9138
                     /locus_tag="J1Y83_003018"
     CDS             8935..9138
                     /locus_tag="J1Y83_003018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HBA7631147.1"
                     /translation="MKNVFKALTVLLTLFSLTGCGLKGPLYFPPADKNAPPPTKPVET
                     QTQSTVPDKNDRATGDGPSQVNY"
     gene            9175..9999
                     /gene="dapF"
                     /locus_tag="J1Y83_003019"
     CDS             9175..9999
                     /gene="dapF"
                     /locus_tag="J1Y83_003019"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HBA7631148.1"
                     /translation="MQFSKMHGLGNDFMVVDAVTQNVFFSPELIRRLADRHLGVGFDQ
                     LLVVEPPYDPELDFHYRIFNADGSEVAQCGNGARCFARFVRLKGLTNKRDIRVSTANG
                     RMVLTVTDDDLVRVNMGEPNFEPSAVPFRANKAEKTYIMRAAEQTILCGVVSMGNPHC
                     VIQVDDVDTAAVETLGPVLESHERFPERANIGFMQVVKREHIRLRVYERGAGETQACG
                     SGACAAVAVGIQQGLLAEEVRVELPGGRLDIAWKGPGHPLYMTGPAVHVYDGFIHL"
     gene            9996..10703
                     /locus_tag="J1Y83_003020"
     CDS             9996..10703
                     /locus_tag="J1Y83_003020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF484 domain-containing protein"
                     /protein_id="HBA7631149.1"
                     /translation="MKQPGEELQDTLTELDDRAVVDYLIKNPEFFIRNARAVEAIRVP
                     HPVRGTVSLVEWHMARARNHIHVLEENMALLMEQAIANEGLFYRLLYLQRSLTAASSL
                     DDMLMRFHRWARDLGLAGASLRLFPDRWRLGAPSNHTHLALSRQSFEPLRIQRLGQEQ
                     HYLGPLNGPELLVVLPEAKAVGSVAMSMLGSDADLGVVLFTSRDASHYQQGQGTQLLH
                     EIALMLPELLARWIERV"
     gene            10700..11596
                     /gene="xerC"
                     /locus_tag="J1Y83_003021"
     CDS             10700..11596
                     /gene="xerC"
                     /locus_tag="J1Y83_003021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HBA7631150.1"
                     /translation="MTDLHTDVERYLRYLSVERQLSPITLLNYQRQLEAIINFASENG
                     LQSWQQCDVTMVRNFAVRSRRKGLGAASLALRLSALRSFFDWLVSQNELKANPAKGVS
                     APKAPRHLPKNIDVDDMNRLLDIDINDPLAVRDRAMLEVMYGAGLRLSELVGLDIKHL
                     DLESGEVWVMGKGSKERRLPIGRNAVAWIEHWLDLRDLFGSEDDALFLSKLGKRISAR
                     NVQKRFAEWGIKQGLNNHVHPHKLRHSFATHMLESSGDLRGVQELLGHANLSTTQIYT
                     HLDFQHLASVYDAAHPRAKRGK"
     gene            11596..12312
                     /gene="yigB"
                     /locus_tag="J1Y83_003022"
     CDS             11596..12312
                     /gene="yigB"
                     /locus_tag="J1Y83_003022"
                     /EC_number="3.1.3.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-amino-6-(5-phospho-D-ribitylamino)uracil
                     phosphatase YigB"
                     /protein_id="HBA7631151.1"
                     /translation="MRFYRPLGRISALTFDLDDTLYDNRPVILRTEREALTFVQNYHP
                     ALRSFQNEDLQRLRQAVREAEPEIYHDVTRWRFRSIEQAMLDAGLSAEEASAGAHAAM
                     INFAKWRSRIDVPQQTHDTLKQLAKKWPLVAITNGNAQPELFGLGHYFEFVLRAGPHG
                     RSKPFSDMYFLAAEKLNVPIGEILHVGDDLTTDVGGAIRSGMQACWIRPENGDLMQTW
                     DSRLLPHLEISRLASLTSLI"
     gene            12396..14558
                     /gene="uvrD"
                     /locus_tag="J1Y83_003023"
                     /gene_synonym="mutU"
                     /gene_synonym="recL"
     CDS             12396..14558
                     /gene="uvrD"
                     /locus_tag="J1Y83_003023"
                     /gene_synonym="mutU"
                     /gene_synonym="recL"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase II"
                     /protein_id="HBA7631152.1"
                     /translation="MDVSYLLDSLNDKQREAVAAPRSNLLVLAGAGSGKTRVLVHRIA
                     WLMSVENCSPYSIMAVTFTNKAAAEMRHRIGQLMGTSQGGMWVGTFHGLAHRLLRAHH
                     MDANLPQDFQILDSEDQLRLLKRLIKAMNLDEKQWPPRQAMWYINSQKDEGLRPHHIQ
                     SYGNPVEQTWQKVYQAYQEACDRAGLVDFAELLLRAHELWLNKPHILQHYRERFTNIL
                     VDEFQDTNNIQYAWIRLLAGDTGKVMIVGDDDQSIYGWRGAQVENIQRFLNDFPGAET
                     IRLEQNYRSTSNILSAANALIENNNGRLGKKLWTDGADGEPISLYCAFNELDEARFVV
                     NRIKTWQDNGGALAECAILYRSNAQSRVLEEALLQASMPYRIYGGMRFFERQEIKDAL
                     SYLRLIANRNDDAAFERVVNTPTRGIGDRTLDVVRQTSRDRQLTLWQACRELLQEKAL
                     AGRAASALQRFMELIDALAQETADMPLHVQTDRVIKDSGLRTMYEQEKGEKGQTRIEN
                     LEELVTATRQFSYNEEDEDLMPLQAFLSHAALEAGEGQADTWQDAVQLMTLHSAKGLE
                     FPQVFIVGMEEGMFPSQMSLDEGGRLEEERRLAYVGVTRAMQKLTLTYAETRRLYGKE
                     VYHRPSRFIGELPEECVEEVRLRATVSRPVSHQRMGTPMVENDSGYKLGQRVRHAKFG
                     EGTIVNMEGSGEHSRLQVAFQGQGIKWLVAAYARLETV"
     gene            complement(14603..15505)
                     /locus_tag="J1Y83_003024"
     CDS             complement(14603..15505)
                     /locus_tag="J1Y83_003024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosyl-l-methionine hydroxide
                     adenosyltransferase family protein"
                     /protein_id="HBA7631153.1"
                     /translation="MKKTLATLFLLTCLGSSSAYADNALILQTDFSLKDGAVSAMKGV
                     AFGVDHNLKIFDLTHEIPPYNIWEGAYRLYQTASYWPQGSVFVSVVDPGVGTDRKSVV
                     LKTKNGQYFVSPDNGTLTLVAESLGIESVRQIDEKTNRLKGSEKSYTFHGRDVYAYTG
                     ARLASGAITFEQVGPELPAKVVELSYQKAKATKGEVKGNIPILDIQYGNVWSNISDEL
                     LNQAGIKLNDTLCVTISEGSQQKYAGKMPYVASFGDVPEGQPMVYLNSLLNVSVALNM
                     DNFAQKHQVASGADWNIDVKKCAK"
     gene            complement(15588..16352)
                     /locus_tag="J1Y83_003025"
     CDS             complement(15588..16352)
                     /locus_tag="J1Y83_003025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiester glycosidase family protein"
                     /protein_id="HBA7631154.1"
                     /translation="MAHRLFIGKGMITLNLKRIFLTLTLLPLFAVAADDCAPSDPTLT
                     VQAYTVNPQTERVKMYWQKANGEAWGTLHALLADINSQGQVQMAMNGGIYDESYAPLG
                     LYIENGQQKVALNLASGEGNFFIRPGGVFYVAGDKVGIVRLDAFKTSKEIQFAVQSGP
                     LLMENGVINPRIHPNVASRKIRNGVGINKHGNAVFLLSQQATNFYDFACYAKAKLNVE
                     QLLYLDGKISHMYMKGEAIPWQRYPFVTMISVEQKG"
     gene            16722..17672
                     /gene="corA"
                     /locus_tag="J1Y83_003026"
     CDS             16722..17672
                     /gene="corA"
                     /locus_tag="J1Y83_003026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium/cobalt transporter CorA"
                     /protein_id="HBA7631155.1"
                     /translation="MLSAFQLENNRLTRLEVEESQPLVNAVWIDLVEPDDDERLRVQS
                     ELGQSLATRPELEDIEASARFFEDDDGLHIHSFFFFEDAEDHAGNSTVAFTIRDGRLF
                     TLRERELPAFRLYRMRARSQSMVDGNAYELLLDLFETKIEQLADEIENIYSDLEQLSR
                     VIMEGHQGDEYDEALSTLAELEDIGWKVRLCLMDTQRALNFLVRKARLPGGQLEQARE
                     ILRDIESLLPHNESLFQKVNFLMQAAMGFINIEQNRIIKIFSVVSVVFLPPTLVASSY
                     GMNFEFMPELKWSFGYPGAIIFMILAGLAPYLYFKRKNWL"
     gene            complement(17708..>18126)
                     /locus_tag="J1Y83_003027"
     CDS             complement(17708..>18126)
                     /locus_tag="J1Y83_003027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000823788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF943 family protein"
                     /protein_id="HBA7631156.1"
                     /translation="QILYTYSDAGNSVFLVVDHLPWTDSDKINWYLKHQDEIKSQNPL
                     PEGSWHTWYVIDIGNGFTDYKKYIEGPYEDLYCFPTIKSNDNCIVKNYLMVINEYPYR
                     NTHFFIADNNEYQLTQENKIERVFNPHDFEKDNFQQ"
BASE COUNT         4193 a         4695 c         4764 g         4474 t
ORIGIN      
        1 cggtgatgtt atatcgcgtt gattattgat gctgttttta gttttaacgg caattaatat
       61 atatgttatt aattgaatga cttttattat tcattatata tatgtgtaga attgtgcgca
      121 ggagaaatat tcactcagga agttattact caggaagtaa agaggattac agaattatct
      181 cataacaagt gttaagggat gttatttccc ggttctctgt ggcataataa acgagtagat
      241 gctcattcca tctcttatgt tcgccttagt gcctcataaa ctccggaatg acgcagagcc
      301 gtttacggtg cttatcgtcc actgacagat gtcgcttatg cctcatcaga caccatggac
      361 acaacgttga gtgaagcacc cacttgttgt catacagacc tgttttaacg cctgctccgt
      421 aataagagca ggcgtttttt tatgaccgag taaacaacag gctacgtcgc tcttgggtca
      481 tcggttgtgc gtttaataca cttacaagag aacctaccgt ggtgatggga agaaacggat
      541 tatcttctat tccgcgtaga tcgcgcccac aacgagcggc aatatcttcg accagctctt
      601 ctaactcgtc tgcatcgttt actaaatcca gatcatcgtc cagtttatca ctggctttca
      661 gtggtaatgg ataatcgata tagccgtttc cttgtaaggt attccacaca gcccgaatca
      721 cccatgtatc tacggtgtgg ggggaaaatt ggcgtgcgaa ctgacaaatg gaaagtccct
      781 gacgtttatc agccataatc tggcgcttct cgatgagctt gattttttcc tgcttcgcaa
      841 tggctatcag atacccaata aaaagtatta accagaagat gaatgtattg cctgtacgta
      901 cgaaggacag aatcagccaa cttacgccag ccagaacggc acatatcaac aaaatcagca
      961 agagcgttgc ccgaaaagga ttaatttcat gtgatggtaa gcggaaaata tagcgggacg
     1021 gtgtgcgcat gtttgacatc cttgtaaaaa aatatcgccc ctgaacgagg cgatatagat
     1081 tactgcggtg ggttgttttg caacgttaac atcaaaccgt cgcgacgcat cgctgcggct
     1141 tcttccggct tgtgcagtct gtccagcgcg tcggcaagcc atgcgtaatc gtaggcgtcc
     1201 ggacgttgtt tcagcgctgc gcggaaggcg agcgatgctt cctgccattc tccgtgtttc
     1261 atcagcgact gacccagtgt gctccacaac agcgggcgat cgcccacgtt tttgatctgt
     1321 tggcgcagca ctttttccag ctgttccggg ttattggttt tcagacgcgg gatcggcagc
     1381 agcaggcgat cgtcatactg gcgtttcagg ccatcgatga taatttgctg ggcagtatca
     1441 tgatcgtcac actcaataag atgttccgcc attgccacct gcaaggccac ctgatgacgc
     1501 gttttccggc tttggttttt ccaccagtta cgcaaacctt cactaccgtt atcggcacgc
     1561 gcctgatcca tcaggccaat ccatgcctgt tgttccagca ttgcacgatg ttcttcatca
     1621 ccaacatggg ctttcgccat tgatgggatg atatccagca gcgaactcca tgcaccagtg
     1681 cggatatacg cctgttccgc cagacgtaat acttccggat ggcgtggcgt aacttccagc
     1741 agcttatcca cgccgtgacg tgctgcatgg ttttcattac gggccagttg cagacgtaca
     1801 cgggtgattt ctaccggaat ggtgtcattg ccggccagct ccgctgcgcg ttccagatgt
     1861 tggttggcgc gtgcttcatc accacgttgt tgcgcggctt cggctgccag tagatagttc
     1921 accaccggtt gttcagcgtg atcggcattt ttcgccatca gcttttcaac ttgctgataa
     1981 tcgccttccg ccagtttcag cagcgcctgt tcggtctgct tacgtgcacg gcgacgctta
     2041 cgtccgacaa accacccacg ggtgtgcgcg cctgtgcgga agattcgccg cagtagccac
     2101 tcaatggcaa acagcactac catcgccaga atcaaaataa tcgcgaggcc cgtgacgcta
     2161 gtttcaatat tgtagttgtc ggtctggatc agcacgtaac cctgatggcc ggcaatcatc
     2221 gggccaacca cgatccccgc aatcagcaac acaaagagca ataacacttt tagcatggtt
     2281 attctccttg cggcgcggct gccggagcat cagcttgcgg tgcaggtgca ggtgcaggtg
     2341 caggtttagc ttccgttgtc cctgctgccg gttgtgccag caggttacgc acgcgggtct
     2401 gcatcaattt ttccagcatc gcctggcttt gcagggtttc cggaagatcc atcgagatat
     2461 tttgctggct taactggtcc acctcgtcga ggaacgcttt ggtggtggca tcatcagtat
     2521 cgtagtaagc acgtacccag gtggagacgt tctccagcgc ctggcgataa gtctcttcct
     2581 ggtgacgcgg tacagcttgt gctgcgacca gcaggcgaga gcgaatattt tcgcgcagat
     2641 agatatcctg atttggcgct aacagcggta cggcggtgtc atcacgacgg cgaatcgtaa
     2701 tgaagttgtc cataaagttc tgccagcttt tttgcagatt gatacgccat tcgctgatgg
     2761 aactggaaag ctcttcaccg tcggaatcca tcggcgaacc atcgctgtca ttatcggcca
     2821 gacgcagatt atctacctga tttgaaagct gattaagctt aaggatgata ccgtcataat
     2881 ccacctgcga taccgcagaa aggctggcga tatcatcggt aattgcccgg cgaacggtaa
     2941 tcagactcgg gtcattcata tccgccaggc tggcgtctgc acttttcagc aacgctgcag
     3001 cggtcgtgac gtcctgatcg ctccacagct tccgtccggc gagtttcacc agaaaatcgg
     3061 cctgggccag cagccaggtt ttagcatcgc tgccggaaat ggtggcgacc ttttgttgga
     3121 cttcatccag ctgttttgcc agcgtttctt gctgacgatt cgcctgctca agctgtacag
     3181 cttgttgctt gataatgcct tccagctcgg ctttttggct ctcctgggct ttttgcagtg
     3241 ccgtaagttg gttagccagg gcatcgctgg tggcagtctg attgacggcc tgttgtttac
     3301 cccagccata caaaccgacg cccgccgcca gagcaatagc gatagccacc gcgctgagaa
     3361 tcaatgcggt attgttctta ctcttttttt ctgttgcgac aggttgtgac gtggtgtcca
     3421 cggcctccct ggtctcttca accacggcgg aggttttttc ttgttccgtc attatggctt
     3481 cctgttatga gagttattgt aatgcccgta aaagcgcatc gttgtcagcg ttatcggcga
     3541 ccttaatgtc ttgccagccc agttcccggg cgagtttcgc caaacgctca ctgacgacca
     3601 atagtcgaca gcgtagtaac cagtgctcac gataccattg tgggatcagc gaccagagtt
     3661 gctgcaacat ttcaccgctg gtaacaacga ccgtcgtcac ctcgcgggat tgccagcgca
     3721 tcgcttcttc tgcaccatcg taatggattg cgcatcgttg ataacattca caaaaagtga
     3781 cctcagcacc gcgcgccgtc agggaatccc taattagctc acgaccgcca ttgccacgta
     3841 atatcagcgc acgtttgccc gcaatatttt gtaattcagg taattgtagc aagacttcgc
     3901 tgatttcccg atcctgcggg tagagaatct tctgtccgct tacggtatgt agtgccagcg
     3961 cggtggtgcg tccaatggcg aaataatcag gtagtcgggg ccatttacga tcttgctgat
     4021 gcagctgtga ttgggcaaaa gcaaccgcgt gttgcgagag ggcaaacaac agatcgctct
     4081 cccccagcgc cgccagttga tcagcaagtt gtggtaattg tcgacccgga gaaaactcaa
     4141 tcagcggaaa atgccaggcc acctgcccca gtgtgcgcag acggctcact aactcttctc
     4201 ctgcgggaga cggacgggtg accaggatac tcatgccggg gcgtctccgt tatagacttc
     4261 agcgaggatc tcgcgcgcgc cgttattcag tagctcttct gccagcgaaa tccccatttg
     4321 ttcggcatct tgcggcgcac cgcggcgttc accgcgaata atctgcgaac cgtccggcgc
     4381 gccaaccaac gcacgcagcc agatttcgcc atcaataagc tcggcgtagc taccaattgg
     4441 cacctgacat ccgccttcga gacgggtatt catggcgcgt tcggcggtaa cgcgcagtgc
     4501 agtttcgtgg tgattcagcg cggcgagcag ctcgcgagtg cgtgcatcat caaggcggca
     4561 ttcaatgccc accgcacctt gccctaccgc cggaagagaa atctcgggtg gcaacgcggc
     4621 gcgaatgcgg gactccagac ctaaacgttt tagtccggct acggcaagaa tgatggcatc
     4681 atattcgccg ttatccagtt tgctcagacg agtgccgacg ttgccgcgca gggagcggat
     4741 aatcagatcc gggcggcgtt cagccagttg gcactggcga cgtaaactgg acgtcccgac
     4801 gatactgcct gccggtagcg catccagact gtcataggtg ttggatacaa aggcatcgcg
     4861 gggatcttca cgctcgcaaa tagtgaccag ccccagacct tgcgggaatt caaccggcac
     4921 atctttcatt gaatgtacgg cgatatcggc gcgattttcg aggagcgcga cttccagctc
     4981 tttcacaaat aagccttttc cgcctacttt cgccagcggc gtatcaagaa tcacatcgcc
     5041 gcgcgtcacc atcggtacca gttcaacgac caggcccgga tggctcgcca tcaacttgtc
     5101 tttgacatag tgtgcctgcc agagtgcaag tgggctttgg cgtgtggcaa ttcttaaaac
     5161 attgtctaac atgcttgtta ccgtcattat catccgtggt ccatcctaac atccttgcca
     5221 gagcgatgtc agtgttgtgg tgaaacgtag acgcctgcgc aaaccgtaaa atgaggtctg
     5281 gcagtggatc ctgataggcg ctttgagcct tcgtaataag gaatttacag agaataaacg
     5341 gtgctacact tgtatgtagc gcatctttct ttacggtcaa tcagcaaggt gttaaattga
     5401 tcacgtttta gaccattttt tcgtcgtgaa actaaaaaaa ccaggcgcga aaagtggtaa
     5461 cggttacctt tgacatacga aatatcccga atgccgcgtg ttaccgttga tgttggcgga
     5521 atcacagtca tgacgggtag caaatcaggc gatacgtctt gtacctctat attgagactc
     5581 tgaaacagag actggatgcc ataaatcaat tgcgtgtgga tcgcgcgctt gctgctatgg
     5641 ggcctgcatt ccaacaggtc tacagtctac tgccgacatt gttgcactat caccatccgc
     5701 taatgccggg ttaccttgat ggtaacgttc ccaaaggcat ttgcctttac acgcctgatg
     5761 aaactcaacg ccactacctg aacgagcttg aactgtatcg tggaatgtca gtacaggacc
     5821 caccgaaagg tgagcttcca attactggtg tatacaccat gggcagcacc tcgtccgtag
     5881 ggcaaagttg ttcctctgac ctggatatct gggtctgtca tcaatcctgg ctcgatagcg
     5941 aagagcgcca gttgctacaa cgtaaatgta gtctgctgga aagctgggcc gcctcgctgg
     6001 gtgtggaagt cagtttcttc ctgattgatg aaaaccgttt ccgtcataat gaaagcggca
     6061 gcctgggggg cgaagattgt ggctccaccc agcatatact gctgcttgac gaattttatc
     6121 gtaccgccgt gcgtctcgcc ggtaagcgta ttctgtggaa tatggtgccg tgcgacgaag
     6181 aagagcatta cgacgactat gtgatgacgc tttacgcgca gggcgtgctg acgccaaatg
     6241 aatggctgga tctcggtggc ttaagctcgc tttctgctga agagtacttt ggtgccagcc
     6301 tttggcagct ctacaagagt atcgattccc catacaaagc ggtgctgaaa acactgctgc
     6361 tggaagccta ctcctgggaa tacccgaacc cacgtctgct ggcgaaagat atcaaacagc
     6421 gtttgcacga cggcgagatt gtatcgtttg gcctcgatcc atactgcatg atgctggagc
     6481 gtgttactga atatctgacg gctattgaag atttcacccg tctggattta gttcgccgct
     6541 gcttctattt aaaagtgtgt gaaaaactta gccgtgaacg cgcctgcgta ggctggcgtc
     6601 gtgaagtgtt aagccagttg gtgaaagagt gggaatggga cgattctcgt ctggcaatgc
     6661 ttgataaccg cgctaactgg aagatagatc aggtgcgtga ggcgcacaac gagctgctcg
     6721 acgcaatgat gcagagctac cgtaatctga tccgctttgc gcgtcgcaat aaccttagcg
     6781 tctccgccag cccgcaggat attggcgtac tgacgcgtaa actgtatgcc gcgtttgaag
     6841 cgttaccggg taaagtgacg ctggtaaacc cgcagatttc gcccgatctc tcggaaccta
     6901 atctgacctt tatttatgtg ccgccgggcc gcgccaatcg ttccggttgg tatctgtata
     6961 accgtgcgcc aacgattgag tcgatcatca gccatcaacc gctggaatat aaccgttatc
     7021 ttaataagct agtggcctgg gcgtggttta acggcctgct gacctcgcgc acccgtttgt
     7081 atattaaagg taacggcatt gtcgatttgc ctaagttgca ggagatggtc gccgacgtgt
     7141 cgcaccattt cccgctgcgc ttacctgcac cgacaccgaa ggcgctctac agcccgtgtg
     7201 agatccgcca tctggcgatt atcgttaacc tagaatatga cccgacagct gcgttccgca
     7261 atcaggtggt gcattttgat ttccgtaagc tggacgtctt cagctttggc gagaatcaaa
     7321 attgcctggt aggtagcgtt gacctgctgt accgcaactc gtggaacgaa gtgcgtacgc
     7381 tgcacttcaa cggcgagcaa tcgatgatcg aagccctgaa aactattctc ggcaaaatgc
     7441 atcaggacgc cgcaccgcca gatagcgtgg aagtcttctg ttatagccag catctgcgcg
     7501 gcttaatccg tactcgcgtg cagcaactgg tttctgagtg tattgaattg cgtctttcca
     7561 gcacccgcca ggaaaccggg cgtttcaagg cgctgcgcgt ttctggtcaa acctggggct
     7621 tgttcttcga acgcctgaat gtatcggtac agaaactgga aaacgccatc gagttttatg
     7681 gcgctatttc gcataacaaa ctgcacggcc tgtcagtgca ggttgaaacc aatcacgtca
     7741 aattaccggc ggtagtggac ggctttgcca gcgaagggat catccagttc ttcttcgaag
     7801 aaacgcaaga cgagaatggc tttaatatct acattctcga cgaaagcaac cgggttgagg
     7861 tgtatcacca ctgcgaaggc agcaaagagg agctggtgcg tgacgtcagt cgcttctact
     7921 cgtcatcgca cgaccgcttc acctacggct caagcttcat caacttcaat ctgccgcagt
     7981 tctatcagat tgtgaaggtt gatggtcgtg aacaggtgat tccgttccgc acaaaatcta
     8041 tcggtaacat gccgcctgcc aatcaggatc acgacacgcc gctattacag cagtattttt
     8101 cgtgatgagc gtgccggaaa gcgaggctta tccggcatgc aatcttagcg gaaactgact
     8161 gtttcacccg cctgctgcgt cgccgcctgt tccagcaaat cccagaaggt ttcgccgctg
     8221 cgatcgcaaa tccactcatc gcctttcata tcaaaatggt agccgccctg tttggttgcc
     8281 agccatacct ggtgcagcgg ttcctggcgg ttgataatga ttttgctccc gttttcaaag
     8341 gtaatggtca gtacaccgcc gttgatttcg cagtcgatat cgctgtcgcc atcccagtcg
     8401 tccagatgtt cttcaatggt cagccacagt tgatcagcta ggcgatgaaa ttcactgtcg
     8461 ttcattgttg tatcctgttt ttaagtgatg gcggcagtat agcggcatgg ggtcagggct
     8521 tcaaagtttg cccgtctgct gctgcgttcc ggcatgattc atccgtcacc ggaataataa
     8581 tatctctata tagcgaaaga ttggtctttt cataagggcg caaatacacc acgccttttc
     8641 cctgtttctg gtaaatatta tgttccggct catcaagccg acatccccat gccgatgata
     8701 agaacaacga acaagacaat aaacaaacca cattgcgata gtgcataaag ccatcctggc
     8761 gcgaggtgcc gatcacgaaa ctaccagcaa aacataaatc gccacgagta agcgttatac
     8821 tcgcagcatt tcctcacttt tcagacttca taaagagtcg ctaaatgctt gcttttacgc
     8881 cttctcctgc gatgatagaa agcagaaagc gatgaacttt acaggcaatc cataatgaaa
     8941 aacgtgttta aggcactcac tgtattactt actctcttca gcctgacggg ctgcggtctg
     9001 aaaggtccgc tctatttccc gcctgcagat aaaaacgcac cgccgccgac caaaccggta
     9061 gagacgcaaa cgcaatccac ggtgccggat aaaaacgacc gcgccactgg cgatggtcca
     9121 tcccaggtga attactaaaa gtcagtttct gtacccgcgt gattggagta aatgatgcag
     9181 ttctcgaaaa tgcatggcct tggcaacgat tttatggtcg tcgacgcggt aacgcagaat
     9241 gtcttttttt caccggagct gattcgtcgc ctggcggatc ggcacctggg ggtagggttt
     9301 gaccaattgc tggtggttga gccgccgtat gatcctgaac tggattttca ctatcgcatt
     9361 ttcaatgctg atggcagtga agtggcgcag tgcggcaacg gcgcgcgctg ctttgcccgt
     9421 tttgtgcgcc tgaaagggct gaccaataag cgcgatatcc gtgtcagcac cgccaacggg
     9481 cggatggttc tgaccgtcac cgatgatgat ctggtccgcg tcaatatggg cgaacccaac
     9541 ttcgaacctt ccgccgtgcc gtttcgcgct aacaaagcgg aaaagaccta tattatgcgc
     9601 gccgccgagc agacaatctt atgcggcgtg gtgtcgatgg gaaatccgca ttgcgtgatt
     9661 caggtcgatg atgtcgatac cgcggcggta gaaacgcttg gtcctgttct ggaaagccac
     9721 gaacgtttcc cggagcgcgc caatatcggt tttatgcaag tggttaagcg cgagcatatt
     9781 cgtttacgcg tttatgagcg tggggcagga gaaacccagg cctgcggcag cggcgcgtgt
     9841 gcggcggttg cagtagggat ccagcaaggt ttgctggccg aagaagtacg cgtggaactc
     9901 cccggcggtc gtcttgatat cgcctggaaa ggtccgggtc acccgttata tatgactggc
     9961 ccggcggtac atgtctacga cggatttatt catctatgaa gcaaccaggg gaagaactgc
    10021 aggatacact cacggagctt gatgaccggg cggttgtcga ttatctgatt aaaaatcctg
    10081 agttttttat ccgcaatgcg cgcgcagtag aagcgatacg tgtgccgcat ccggtacgcg
    10141 gcaccgtttc gttggtcgag tggcacatgg cccgcgcacg taatcatatt catgttctgg
    10201 aagagaacat ggcgttgttg atggaacagg ctattgccaa cgaagggctg ttttatcgtc
    10261 tgctctacct gcagcgcagt ctcaccgccg ccagcagtct cgatgatatg ctgatgcgct
    10321 ttcaccgctg ggcgcgcgat ctcggtctgg cgggcgcgag tctgcgcctg tttccagatc
    10381 gctggcgttt aggtgcgccg tcgaaccaca ctcatctggc attaagccgt cagtctttcg
    10441 aaccgctgcg tattcagcgt ctggggcagg aacagcacta tctggggcca cttaacggac
    10501 cggagcttct ggtggtgcta ccggaagcga aagcggtggg atcggtggcg atgtcgatgc
    10561 tgggaagcga tgctgatttg ggtgttgtgc tgtttaccag tcgcgatgcc agtcactatc
    10621 aacaagggca gggaacgcag ttacttcatg aaattgcgct gatgttgccg gagcttctgg
    10681 cgcgttggat tgaacgcgta tgaccgattt acacaccgat gtagaacgct acctacgtta
    10741 tctgagcgtg gagcgtcagc ttagcccgat aaccctgctt aactatcagc gtcagcttga
    10801 ggcgatcatc aattttgcca gtgaaaacgg cctgcaaagc tggcagcaat gtgatgtgac
    10861 gatggtgcgc aatttcgctg tacgcagccg ccgtaaaggg ctgggagctg caagtctggc
    10921 gttacggctt tctgcgctac gtagcttttt tgactggctg gtcagccaga acgaactcaa
    10981 agccaacccg gcgaaaggtg tttcggcacc gaaagcgccg cgtcatctgc cgaaaaacat
    11041 cgacgtcgat gatatgaatc ggctgctgga tattgatatc aacgatcccc tcgctgtacg
    11101 cgaccgtgca atgctggaag tgatgtacgg cgcgggtctg cgtctttccg agctggtggg
    11161 gctggatatc aaacacctcg acctggagtc tggtgaagtg tgggtgatgg gaaaaggcag
    11221 caaagagcgc cgcctgccga ttggtcgcaa cgccgtggcg tggattgagc actggcttga
    11281 tttgcgcgac ctgtttggta gcgaagacga cgcgcttttt ctgtcgaaac tgggcaagcg
    11341 tatctccgcg cgtaatgtgc agaaacgctt tgccgaatgg ggcatcaaac aagggctgaa
    11401 taatcacgtt cacccgcata aattacgtca ctcgttcgct acgcatatgc tggagtcgag
    11461 cggcgatctt cgtggtgtgc aggagctgct gggtcatgcc aacctctcca ccacgcaaat
    11521 ctatactcat cttgattttc aacaccttgc ctcggtgtac gatgcggcgc atccacgcgc
    11581 caaacggggg aaataatgcg tttttaccgg cctttggggc gcatctcggc gctcaccttt
    11641 gacctggatg atacccttta cgataaccgt ccggtgattt tgcgcaccga gcgagaggcg
    11701 cttacctttg tgcaaaatta tcatccggcg ctgcgcagct tccagaatga agatctgcaa
    11761 cgcctgcgcc aggcggtacg ggaagcggaa cccgagattt atcacgacgt gacgcgctgg
    11821 cgttttcgtt cgattgaaca agcgatgctc gacgccgggc tgagtgccga agaagccagt
    11881 gcaggcgcac atgcagcaat gatcaacttt gccaaatggc gcagccggat cgacgtcccg
    11941 cagcaaaccc acgacacctt aaaacagctg gcgaagaaat ggccgctggt ggcgatcacc
    12001 aacggtaacg cccagccgga actgtttggt ttggggcatt attttgagtt tgtgctgcgc
    12061 gctggcccgc acgggcgctc aaaaccgttc agcgatatgt acttcctggc ggcggaaaaa
    12121 ctcaacgtgc cgatcggcga gatcttacat gtcggggacg atctcaccac tgacgtgggt
    12181 ggggcaattc gcagcggaat gcaggcctgt tggatcagac cggaaaatgg cgatctgatg
    12241 caaacctggg acagccgttt actgccgcat ctggaaattt cccggttggc atctctgacc
    12301 tcgctgatat aatcagcaaa tctgtatata tacccagctt tttggcggag ggcgttgcgc
    12361 ttctccgccc aacctatttt tacgcggcgg tgccaatgga cgtttcttac ctgctcgaca
    12421 gccttaatga caaacagcgc gaagcggtgg ccgcgccacg cagcaacctt ctggtgctgg
    12481 cgggcgcggg cagtggtaag acgcgcgtac tggtgcatcg tatcgcctgg ctgatgagcg
    12541 tggaaaactg ctcgccgtat tcgattatgg cggtgacgtt taccaacaaa gcggcggcgg
    12601 agatgcgcca tcgtatcggg cagctgatgg gcaccagcca gggcggcatg tgggtcggca
    12661 ccttccacgg gctggcgcac cgcctgctgc gtgcgcacca catggacgcc aacctgccgc
    12721 aggatttcca gatcctcgac agcgaggacc agctacgcct gctcaaacgc ctaatcaagg
    12781 cgatgaacct cgacgagaag cagtggccgc cgcgccaggc aatgtggtac atcaacagcc
    12841 agaaagatga aggcctgcgt ccacatcata ttcaaagcta cggtaatccg gtggagcaga
    12901 cctggcagaa ggtgtatcag gcgtatcagg aagcgtgtga ccgcgcgggt ctggttgatt
    12961 ttgctgaatt gctgcttcgt gcccacgaat tatggctcaa caaaccgcat atccttcaac
    13021 attaccgcga acgtttcacc aatattctgg tggatgaatt ccaggacact aacaacattc
    13081 agtacgcgtg gatccgactg ctggcaggcg atactggcaa agtgatgatc gttggcgatg
    13141 acgaccagtc aatctacggc tggcgcgggg cgcaggtcga gaatattcag cgtttcctca
    13201 atgatttccc cggtgctgaa actatccgtc tggagcagaa ctaccgctct accagcaata
    13261 ttctgagcgc cgctaacgcc ctgattgaaa acaataacgg gcgtctgggt aaaaaactgt
    13321 ggaccgatgg cgcggacggt gagcctattt ccctctattg cgcttttaac gaactcgacg
    13381 aagcgcgttt tgtggttaac cgcatcaaaa cctggcagga caacggcggg gcgctcgctg
    13441 agtgcgccat tctctaccgc agcaacgccc agtcgcgtgt gctggaagag gcgttattgc
    13501 aggcgagtat gccgtatcgt atttacggcg ggatgcgctt cttcgaacgc caggaaatca
    13561 aagatgcgct ctcgtatctg cgcctgattg ccaaccgcaa cgacgacgcg gcctttgagc
    13621 gtgtggtgaa tacgccaacg cggggtattg gtgaccggac gctggacgtg gtacgtcaga
    13681 catcgcgaga tcgccagtta acactctggc aggcatgtcg tgaactgttg caggaaaaag
    13741 ccctcgccgg acgtgctgcc agcgccttac agcggtttat ggagctgatc gacgccttag
    13801 cgcaggaaac tgccgatatg ccgctgcatg tacagactga ccgggtaatt aaagactccg
    13861 gcctgcgcac catgtacgag caggagaagg gcgaaaaagg tcagacgcgt atcgaaaact
    13921 tagaggaact ggtgacggca acgcgccagt tcagctacaa cgaagaagac gaagatttaa
    13981 tgccgctgca ggcattcctc tcccatgcgg cgctggaagc gggcgagggg caggcggata
    14041 cctggcagga cgcggtgcag ttgatgacgc tacactcggc gaaaggtctg gaattcccgc
    14101 aggtgtttat cgttggtatg gaagagggca tgttcccaag ccagatgtcg ctggatgaag
    14161 gcgggcgtct ggaagaagaa cgccgtctgg cctacgttgg cgtaacccgc gcgatgcaga
    14221 aactgacgct gacctacgcg gaaacccgcc gtctatatgg caaagaggtt taccatcgcc
    14281 cgtcgcgctt tatcggtgag ttgccggaag agtgtgtgga agaggtgcgc ctgcgcgcca
    14341 cggtaagccg cccggtcagc catcaacgga tggggacgcc gatggtcgag aacgacagcg
    14401 gctacaagct cggccagcgc gtacgccacg ctaagtttgg tgaaggcacc atcgtcaata
    14461 tggaaggcag cggtgagcat agccgtttgc aggtggcgtt ccagggccag ggtattaaat
    14521 ggctggtggc ggcttacgcc cggttggaga cggtgtgaag ttgcggaatg cggtgcgcca
    14581 taccgcattc cgcatcagca ccttacttag cgcacttctt cacatcaata ttccagtcag
    14641 caccagatgc aacctgatgt ttctgcgcga aattatccat attcagcgcc acggaaacat
    14701 tcaataagct gttcagatac accatcggct ggccttccgg cacatcgccg aagcttgcga
    14761 catacggcat tttccctgcg tatttttgct gggaaccttc ggaaatggtc acacacaacg
    14821 tgtcgttgag tttgattcct gcctgattca gcaactcgtc gctgatattg ctccagacgt
    14881 tgccgtactg aatatcgaga atcgggatat tgcccttcac ttcacctttt gttgctttgg
    14941 ctttctgata agaaagttcg acaacttttg caggaagctc tggccccacc tgctcgaacg
    15001 tgatcgcccc ggaagccaga cgtgcaccgg tgtaggcata cacgtcacga ccgtggaagg
    15061 tatatgactt ttcggaacct ttcagacggt tggttttttc atcgatttgc cggacgctct
    15121 caatacccag cgattccgcc accaacgtaa gcgtaccgtt atctggtgaa acgaagtact
    15181 gaccgttttt ggttttcagc actaccgatt tacggtctgt accaacacct ggatcgacca
    15241 cgctaacaaa caccgaacct tgcggccagt aactggcagt ctgatacagg cggtaagcgc
    15301 cttcccagat gttatatggt ggaatttcgt gtgttaaatc aaatatttta agattatggt
    15361 caacgccgaa agcgacccct ttcatcgccg agacggcgcc atctttcagg ctaaaatccg
    15421 tttgtaaaat caaagcgtta tccgcataag cggatgatga ccccagacag gttaataaaa
    15481 ataatgttgc gagcgttttt ttcatttttg tttcccttca tttatctgat gattttccag
    15541 ctgtgtagcc agccccagtt tgtctggcct cctcgagtgt acaatgttta acctttctgc
    15601 tcgacggaaa tcatggtgac aaaaggatag cgttgccacg ggattgcctc gcctttcata
    15661 tacatatgtg aaattttgcc atcaagataa agcagctgct caacgttcag tttcgctttg
    15721 gcataacagg caaaatcata aaaatttgtc gcctgctggc tcaacaaaaa cacggcgttc
    15781 ccatgtttat taatcccaac accgttacga attttacgtg aggcgacgtt gggatgaata
    15841 cgcggattaa ttacaccgtt ttccatcaac aatggccctg actgcaccgc aaactgaatc
    15901 tctttactgg ttttaaaggc atccagacga acgatgccga ctttatctcc cgcgacataa
    15961 aacactccgc caggacgaat aaagaaattc ccttcacctg aagcgagatt taacgccacc
    16021 ttctgctgac cgttttcgat gtacaaaccg agcggcgcat agctttcatc atagatgccg
    16081 ccgttcatcg ccatctgcac ctgaccctga ctattaatat ccgccagcag agcatgtaac
    16141 gttccccacg cttcgccatt ggctttttgc cagtacattt tcacccgctc tgtttgagga
    16201 ttaacggtat acgcctgtac ggtcagcgtc ggatctgagg gtgcgcaatc atcagcggca
    16261 acagcaaaca agggaagtaa ggtgagggtg agaaaaatcc gtttgagatt caaggtgatc
    16321 attcctttac caatgaatag ccgatgcgcc attataggcc ctgaatgtgg gattttttta
    16381 tcctgttagc gaccttgacg agtaccaaaa agcgcgaagt tcaactattg ttctgtggtg
    16441 ttctgttgcg tgttgacggc aaaattttgc tggcgtaaca tgcgcgcacg atcactctaa
    16501 gaggacattc gccttggaca cacccagtag atactggctc attatcctgt catccaggat
    16561 caactcctaa ggctatccct ttttgctgat agccttagcg gttgtcagcg acctcaattt
    16621 ttcccgtcgc gctgagtcag gctgtttaat ggtctgaaac ccaatttgtt tctgtgtgcc
    16681 caccgaactg tccgatattt taagcattgg gagtcccggt catgctgagc gcatttcaac
    16741 tggaaaataa ccgactgacc cggctggaag tcgaagagtc acaacccctt gtaaatgcag
    16801 tatggattga tcttgtcgaa ccggacgacg acgagcgact gcgcgtacaa tctgaacttg
    16861 gccagagcct ggcaacccgc ccggaactgg aagacatcga agcatcggca cgtttctttg
    16921 aagacgatga cggcctgcat attcactcct tcttcttctt tgaagatgcg gaagatcacg
    16981 ccggtaactc cactgtggca tttaccatcc gtgatggtcg tctgtttact ctgcgtgagc
    17041 gtgaactgcc cgcttttcgt ctgtatcgta tgcgtgcccg tagccagtca atggtggacg
    17101 gtaacgccta cgagctgctg ctggatctgt tcgaaaccaa aatcgaacag ttggcagatg
    17161 aaattgaaaa tatctacagc gacctggagc agttgagccg ggtaattatg gaagggcatc
    17221 agggcgatga gtacgacgag gcgctctcca cgctggcgga actggaagat atcggctgga
    17281 aagttcgcct gtgtctgatg gatacccagc gcgcgctcaa cttcctggta cgtaaagcac
    17341 gcttaccggg tgggcaactg gagcaggcgc gtgaaatcct gcgagatatc gaatccctgc
    17401 tgccgcataa cgaatctcta ttccagaagg tgaacttcct gatgcaggcg gcgatgggct
    17461 ttatcaatat cgagcagaac cgcatcatca aaatcttctc ggtggtatcc gtggtgttcc
    17521 tgccaccgac gctggtcgct tccagctacg ggatgaactt tgagtttatg ccggaactga
    17581 agtggagctt cggctaccca ggcgcgatta tctttatgat cctcgcgggc ctggcaccgt
    17641 atctgtactt taagcggaag aactggttgt aatataaatg ccgggtaaag attgttcttt
    17701 atccggctta ttgctgaaaa ttgtcttttt caaagtcatg cgggttgaac actcgctcta
    17761 tcttattttc ctgggttagt tgatactcat tgttatcagc aataaaaaaa tgagtattac
    17821 ggtaaggata ttcattaata accattaaat agttttttac gatgcaatta tcattgctct
    17881 taatcgtcgg gaaacagtag agatcttcat acggcccttc gatatatttt ttatagtcag
    17941 tgaagccatt accgatatca ataacatacc aggtatgcca actaccttct ggtaggggat
    18001 tttgactttt aatttcatcc tgatgcttca gataccaatt tattttatcg ctatcagtcc
    18061 atggcagatg atcaacgacc agaaagactg aattgccagc atcagagtat gtatacaaaa
    18121 tctgta
//
LOCUS       DADSYM010000104        17657 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2415
            SAMN08039579-rid11723173.denovo.104, whole genome shotgun sequence.
ACCESSION   DADSYM010000104 DADSYM010000000
VERSION     DADSYM010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039579
            Sequence Read Archive: SRR6362533
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17657)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17657)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980162.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 03:39:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,484
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 4,347
            CDSs (with protein)               :: 4,347
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 43 of 85
            Pseudo Genes (incomplete)         :: 37 of 85
            Pseudo Genes (internal stop)      :: 19 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17657
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039579-rid11723173.denovo.104"
                     /strain="LGE2415"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8302 N 95.9628 W"
                     /collection_date="2015-08-24"
     gene            <1..74
                     /gene="tnaC"
                     /locus_tag="J1Y83_003028"
     CDS             <1..74
                     /gene="tnaC"
                     /locus_tag="J1Y83_003028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tryptophanase leader peptide"
                     /protein_id="HBA7631157.1"
                     /translation="NILHICVTSKWFNIDNKIVDHRP"
     gene            295..1710
                     /gene="tnaA"
                     /locus_tag="J1Y83_003029"
     CDS             295..1710
                     /gene="tnaA"
                     /locus_tag="J1Y83_003029"
                     /EC_number="4.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709490.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophanase"
                     /protein_id="HBA7631158.1"
                     /translation="MENFKHLPEPFRIRVIEPVKRTTRAYREEAIIKSGMNPFLLDSE
                     DVFIDLLTDSGTGAVTQSMQAAMMRGDEAYSGSRSYYALAESVKNIFGYQYTIPTHQG
                     RGAEQIYIPVLIKKREQEKGLDRSKMVAFSNYFFDTTQGHSQINGCTVRNVYIKEAFD
                     TGVRYDFKGNFDLEGLERGIEEVGPNNVPYIVATITSNSAGGQPVSLANLKAMYSIAK
                     KYDIPVVMDSARFAENAYFIKQREAEYKDWTIEQITRETYKYADMLAMSAKKDAMVPM
                     GGLLCMKDDSFFDVYTECRTLCVVQEGFPTYGGLEGGAMERLAVGLYDGMNLDWLAYR
                     IAQVQYLVDGLEEIGVVCQQAGGHAAFVDAGKLLPHIPADQFPAQALACELYKVAGIR
                     AVEIGSFLLGRDPKTGKQLPCPAELLRLTIPRATYTQTHMDFIIEAFKHVKENAANIK
                     GLTFTYEPKVLRHFTAKLKEV"
     gene            1802..3049
                     /gene="tnaB"
                     /locus_tag="J1Y83_003030"
     CDS             1802..3049
                     /gene="tnaB"
                     /locus_tag="J1Y83_003030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low affinity tryptophan permease TnaB"
                     /protein_id="HBA7631159.1"
                     /translation="MTDQSEKKHSAFWGVMVIAGTVIGGGMFALPVDLAGAWFFWGAF
                     ILIIAWFSMLHSGLLLLEANLNYPVGSSFNTITKDLIGNTWNIISGITVAFVLYILTY
                     AYISANGAIISETISMNLGYHANPRIVGICTAIFVASVLWISSLAASRITSLFLGLKI
                     ISFVIVFGSFFFQVDYSILRDSTSTTAGTSYFPYIFMALPVCLASFGFHGNIPSLIIC
                     YGKRKDKLIKSVVFGSLLALVIYLFWLYCTMGNIPRESFKAIISSGGNVDSLVKSFLG
                     TKQHGIIEFCLLVFSNLAVASSFFGVTLGLFDYLADLFKIDNSHGGRFKTVLLTFLPP
                     ALLYLIFPNGFIYGIGGAGLCATIWAVIIPAVLAIKARKKFPNQMFTVWGGNLIPAIV
                     ILFGITVILCWFGNVFNVLPKFG"
     gene            3181..4356
                     /gene="mdtL"
                     /locus_tag="J1Y83_003031"
     CDS             3181..4356
                     /gene="mdtL"
                     /locus_tag="J1Y83_003031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtL"
                     /protein_id="HBA7631160.1"
                     /translation="MSRFLICSFALVLLYPAGIDMYLVGLPRIAADLNASEAQLHIAF
                     SVYLAGMAAAMLFAGKVADRSGRKPVAIPGAALFIIASVFCSLAETSALFLAGRFLQG
                     LGAGCCYVVAFAILRDTLDDRRRAKVLSLLNGITCIIPVLAPVLGHLIMLKFPWQSLF
                     WTMATMGIAVLMLSLFILKETRPAAPAASDKPRENSESLLNRFFLSRVVITTLSVSVI
                     LTFVNTSPVLLMEIMGFERGEYATIMALTAGVSMTVSFSTPFALGIFKPRTLMITSQV
                     LFLAAGITLAVSPSHAVSLFGITLICAGFSVGFGVAMSQALGPFSLRAGVASSTLGIA
                     QVCGSSLWIWLAAVVGIGAWNMLIGILIACSIVSLLLIMFVAPGRPVAAHEEIHHHA"
     gene            4331..5290
                     /gene="yidZ"
                     /locus_tag="J1Y83_003032"
     CDS             4331..5290
                     /gene="yidZ"
                     /locus_tag="J1Y83_003032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator YidZ"
                     /protein_id="HBA7631161.1"
                     /translation="MKKSITTLDLNLLLCLQLLMQERSVTKAAKRMSVTPSAVSKSLA
                     KLRMWFDDPLFVNSPLGLSPTPLMVSMEQNLAEWMQMSNQLLDKPHHQTPRGLKFELA
                     AESPLMMIMLNALSKRIYQRYPQATIKLRNWDYDSLDAITRGEVDIGFSGRESHPRSR
                     ELLSSLPLAIDYEVLFSDVPCVWLRKDHPALHEAWNLDTFLRYPHISICWEQSDTWAL
                     DNVLQELGRERTIAMSLPEFEQSLFMAAQPDNLLLATAPRYCQYYNQLHQLPLVALPL
                     PFDESQQKKLEVPFTLLWHKRNSRNPKIVWLRETIKNLYASMA"
     gene            5447..6196
                     /locus_tag="J1Y83_003033"
     CDS             5447..6196
                     /locus_tag="J1Y83_003033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA7631162.1"
                     /translation="MATHFARGILTEGHLISVRLPSQCHQEARNIPPHRQSRFLASRG
                     LLAELMFMLYGIGELPEIVTLPKGKPVFSDKNLPSFSISYAGNMVGVALTTEGECGLD
                     MELQRATRGFHSPHAPDNHTFSSNESLWISKQNDPNEARAQLITLRRSVLKLTGDVLN
                     DDPRDLQLLPIAGRLKCAHVNHVEALCDAEDVLVWSVAVTPTIEKLSVWELDGKHGWK
                     SLPDIHSRANNPTSRMMRFAQLSTVKAFSPN"
     gene            6218..6784
                     /gene="yieF"
                     /locus_tag="J1Y83_003034"
     CDS             6218..6784
                     /gene="yieF"
                     /locus_tag="J1Y83_003034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent chromate oxidoreductase YieF"
                     /protein_id="HBA7631163.1"
                     /translation="MSEKLQVVTLLGSLRKGSFNGMVARTLPKFAPASMEINALPSIA
                     DIPLYDADVQQEDGFPATVEALAEQIRQADGVVIVTPEYNYSVPGGLKNAIDWLSRLP
                     DQPLAGKPVLIQTSSMGVIGGARCQYHLRQILVFLDAMVMNKPEFMGGVIQNKVDPQT
                     GEVIDQGTLDHLTGQLTAFGEFIQRVKI"
     gene            complement(6838..8175)
                     /locus_tag="J1Y83_003035"
     CDS             complement(6838..8175)
                     /locus_tag="J1Y83_003035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HBA7631164.1"
                     /translation="MSQQHTTQASGQGMLERVFKLREHGTTARTEVIAGFTTFLTMVY
                     IVFVNPQILGVAGMDTSAVFVTTCLIAAFGSIMMGLFANLPVALAPAMGLNAFFAFVV
                     VQAMGLPWQVGMGAIFWGAIGLLLLTIFRVRYWMIANIPVSLRVGITSGIGLFIGMMG
                     LKNAGVIVANPETLVSIGNLTSHSVLLGILGFFIIAILASRNIHAAVLVSIVVTTLLG
                     WILGDVHYNGIVSAPPSVMTVVGHVDLAGSFNLGLAGVIFSFMLVNLFDSSGTLIGVT
                     DKAGLADEKGKFPRMKQALYVDSISSVTGSFIGTSSVTAYIESSSGVSVGGRTGLTAV
                     VVGLLFLLVIFLSPLAGMVPGYAAAGALIYVGVLMTSSLARVNWQDLTESVPAFITAV
                     MMPFSFSITEGIALGFISYCVMKIGTGRLRDLSPCVIIVALLFILKIVFIDAH"
     gene            8341..9006
                     /gene="yieH"
                     /locus_tag="J1Y83_003036"
     CDS             8341..9006
                     /gene="yieH"
                     /locus_tag="J1Y83_003036"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconate phosphatase"
                     /protein_id="HBA7631165.1"
                     /translation="MSQIEAVFFDCDGTLVDSEVICSRAYVTMFREFGIHVDLEEIFT
                     RFKGVKLYEIIDIISKEQGVTMVQADAEHVYRAEVARLFDTELEDIAGANELLASINV
                     PMCVVSNGPVSKMQHSLGKLNMLHYFPEKLFSGYDIQRWKPDPALMFHAAKAMNVNVE
                     NCILVDDSSAGAQSGIDAGMEVFYFCADPHNKPIVHPKVTTFTHLSQLPELWKARGWD
                     ITA"
     gene            9073..9546
                     /locus_tag="J1Y83_003037"
     CDS             9073..9546
                     /locus_tag="J1Y83_003037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA7631166.1"
                     /translation="MSVSRRVIHHGLYFAVLGPLIGVLFLVLYIFFAKEPLILLVIIQ
                     VLPLFLLMSITTGAIPAMLTGVMVACLPEKIGSQKRYRCLVGGIGGVVITEIYCAVIV
                     HIKDMASSALFENILSGENLVVRIIPALLAGVLMSRIITRLPGLDISCPETDSLS"
     gene            complement(9628..10350)
                     /locus_tag="J1Y83_003038"
     CDS             complement(9628..10350)
                     /locus_tag="J1Y83_003038"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HBA7631167.1"
                     /translation="MKLIITEDYQEMSRVAAHHLLGYMSKTRRVNLAITAGSTPKGMY
                     EYLITLVKGKPWYDNCYFYNFDEIPFRGKEGEGVTITNLRNLFFTPAGIKEENIQKLT
                     IDNYREHDQKLAREGGLDLVVLGLGVDGHFCGNLPNTTHFHEQTVEFPIQGEMVDIVA
                     HGELGGDFSLVPDSYVTMGPKSIMAAKNLLIIVSGAGKAQALKNVLQGPVTEDVPASV
                     LQLHPSLMVIADKAAAAELALG"
     gene            complement(10365..11533)
                     /locus_tag="J1Y83_003039"
                     /pseudo
     CDS             complement(10365..11533)
                     /locus_tag="J1Y83_003039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709476.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
     gene            complement(11560..13176)
                     /locus_tag="J1Y83_003040"
     CDS             complement(11560..13176)
                     /locus_tag="J1Y83_003040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate porin"
                     /protein_id="HBA7631168.1"
                     /translation="MFRRNIITSAILLMAPLAFSAQSLAESLTVEQRLELLEKALRET
                     QSELKKYKDEEKKKYTPATVNRSVSTNDQGYAANPFPTSRAAKPDAVLVKNEEKNASE
                     TGSIYSSMTLKDFSKFVKDEIGFSYNGYYRSGWGTASHGSPKSWAIGSLGRFGNEYSG
                     WFDLQLKQRVYNENGKRVDAIVMMDGNVGQQYSTGWFGDNAGGENFMQFSDMYVTTKG
                     FLPFAPEADFWVGKHGAPKIEIQMLDWKTQRTDAAAGVGLENWKVGPGKIDIALVRED
                     VDDYDRSLQNKQQINTNTIDLRYKDIPLWDKATLMVSGRYVSANESASEKDNQDNNGY
                     YDWKDTWMFGTSLTQKFDKGGFNEFSFLVANNSIASNFGRYAGASPFTTFNGRYYGDH
                     TGGTAVRLTSQGEAYIGDHFIVANAIVYSFGNDIYSYETGAHSDFESIRAVVRPAYIW
                     DQYNQTGVELGYFTQQNKDANSNKYNESGYKTTLFHTFKVNTSMLTSRPEIRFYATYI
                     KALENELDGFTFEDNKDDQFAVGAQAEIWW"
     gene            complement(13262..14656)
                     /locus_tag="J1Y83_003041"
     CDS             complement(13262..14656)
                     /locus_tag="J1Y83_003041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HBA7631169.1"
                     /translation="MKAFPETFLWGGATAANQVEGAWQEDGKGITTSDLQPHGVMGKM
                     EPRILGKENIKDVAIDFYHRYPEDIALFAEMGFTCLRISIAWARIFPQGDEAEPNEAG
                     LAFYERLFDEMAQAGIKPLVTLSHYEMPYGLVKNYGGWANRAVIDHFEHYARTVFTRY
                     QHKVALWLTFNEINMSLHAPFTGVGLAEESGEAEVYQAIHHQLVASARAVKACHSLIP
                     EAKIGNMLLGGLVYPLTCQPQDMLQAMEENRRWMFFGDVQARGQYPGYMQRFFRDHNI
                     TIEMTESDAEDLKHTVDFISFSYYMTGCVSHDESINKNAQGNILNMIPNPHLKSSEWG
                     WQIDPVGLRVLLNTLWDRYQKPLFIVENGLGAKDSVEADGSIQDDYRIAYLNDHLVQV
                     NEAIADGVDIMGYTSWGPIDLVSASHSQMSKRYGFIYVDRDDNGEGSLTRTRKKSFGW
                     YAEVIKTRGLSLKK"
     gene            complement(14675..16549)
                     /gene="bglF"
                     /locus_tag="J1Y83_003042"
     CDS             complement(14675..16549)
                     /gene="bglF"
                     /locus_tag="J1Y83_003042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS beta-glucoside transporter subunit IIABC"
                     /protein_id="HBA7631170.1"
                     /translation="MTELARTIVAGVGGADNIVSLMHCATRLRFKLKDESKAQAEVLK
                     KTPGIIMVVESGGQFQVVIGNHVADVFLAVNSVAGLGEKAQQAPENDDKDNLLNRFVY
                     VISGIFTPLIGLMAATGILKGMLALALTFQWATEQSGTYLILFSASDALFWFFPIILG
                     YTAGKRFGGNPFTAMVIGGALVHPLILTAFENGQKVDALGLDFLGIPVTLLNYSSSVI
                     PIIFSAWLCSILERRLNAWLPSAIKNFFTPLLCLMVITPITFLLVGPLSTWISELIAA
                     GYLWLYQAVPAFAGAVMGGFWQIFVMFGLHWGLIPLIINNFTVLGYDTMIPLLMPAIM
                     AQVGAALGVFLCERDAQKKVVAGSAALTGLFGITEPAVYGVNLPRKYPFVIACISGAL
                     GATIIGYAQTKVYSFGLPGIFTFMQTIPSTGIDFTVWASVIGGVIAIGCAFVGTVMFH
                     FITAKSQSAQVAQQEKTPEVITPEQGGICSPMTGEIVPLIHVADTTFASGLLGKGIAI
                     LPSVGEVRSPVAGRIASLFATLHAIGIESDDGVEILIHVGIDTVKLDGKFFSAHVNVG
                     DKVNTGDRLISFDIPAIREAGFDLTTPVLISNSDDFTDVLPHGTAQINAGEPLLSIIR
                     "
     gene            complement(16683..17519)
                     /gene="bglG"
                     /locus_tag="J1Y83_003043"
     CDS             complement(16683..17519)
                     /gene="bglG"
                     /locus_tag="J1Y83_003043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional antiterminator BglG"
                     /protein_id="HBA7631171.1"
                     /translation="MNMQITKILNNNVVVVIDDQQREKVVMGRGIGFQKRPGERINSS
                     GIEKEYALSSHELNGRLSELLSHMPLEVMATCDRIISLAQERLGKLQDSIYISLTDHC
                     QFAIKRFQQNVLLPNPLLWDIQRLYPKEFQLGEEALTIIDKRLGVQLPKDEVGFIAMH
                     LVSAQMSGNMEDVAGVTQLMREMLQLIKFQFSLNYQEESLSYQRLVTHLKFLSWRIIE
                     HASINDSDESLQQAVKQNYPQAWQCAERIAIFIGLQYQRKISPAEIMFLAINIERVRK
                     EH"
BASE COUNT         4486 a         4443 c         4108 g         4620 t
ORIGIN      
        1 tgaatatctt acatatatgt gtgacctcaa aatggtttaa tattgacaac aagattgtcg
       61 atcaccgccc ttgatttgcc cttctgtagc catcaccaga gccaaaccga ttagattcaa
      121 tgtgatctat ttgtttgcta tatcttaatt ttgcctttta caaaggccat ctctcgttta
      181 tttacttgtt ttagtaaatg atggtgcttg catatatatc tggcgaatta atggatactg
      241 caaatgtaat attcacaggg ttcactgtaa ttaaaataaa tgaaggatta tgtaatggaa
      301 aactttaaac atctccctga accgttccgc attcgtgtta ttgagccagt aaaacgtacc
      361 actcgcgctt atcgtgaaga ggcaattatt aaatccggta tgaacccgtt cctgctggat
      421 agcgaagatg tgtttatcga tttactgacc gacagcggca ccggggcggt gacgcagagt
      481 atgcaggccg cgatgatgcg cggcgacgaa gcttacagcg gcagccgcag ctactacgcg
      541 ttagccgagt cagtgaaaaa tatctttggt tatcaataca ctattccgac tcaccagggc
      601 cgtggtgcag agcaaatcta tattccggta ctgattaaaa aacgcgagca ggaaaaaggc
      661 ctggatcgca gcaaaatggt ggcgttctct aactatttct ttgataccac gcagggccat
      721 agccagatta acggctgtac cgtgcgtaac gtctatatca aagaagcctt cgatactggc
      781 gtgcgttacg actttaaagg caactttgac cttgagggat tagaacgcgg tattgaagaa
      841 gttggcccga ataacgtgcc gtatatcgtt gcaaccatca ccagtaactc cgcaggtggt
      901 cagccggttt cactggcaaa cttaaaagcg atgtacagca tcgcgaagaa atacgatatt
      961 ccggtggtca tggactccgc acgctttgcc gaaaacgcct atttcatcaa gcagcgtgaa
     1021 gcagaataca aagactggac catcgagcag atcacccgcg aaacctacaa atatgccgat
     1081 atgctggcga tgtccgccaa gaaagatgcg atggtgccga tgggcggcct gctgtgcatg
     1141 aaagacgaca gcttctttga tgtgtatacc gaatgcagaa ccctttgcgt ggtgcaggaa
     1201 ggcttcccga catatggcgg cctggaaggc ggcgctatgg agcgtctggc ggtaggtctg
     1261 tatgacggca tgaatctgga ctggctggct tatcgtatcg cgcaggtgca gtatctggtc
     1321 gatggtctgg aagagattgg cgttgtctgt cagcaggcgg gcggtcacgc ggcattcgtt
     1381 gatgcaggta aactgttgcc gcatatcccg gcagatcagt tcccggcaca ggcgctggcc
     1441 tgcgagctgt ataaagtcgc cggtatccgt gcggtagaaa ttggctcttt cctgttaggc
     1501 cgcgatccga aaaccggtaa acaactgcca tgcccggctg aactgctgcg tttaaccatt
     1561 ccgcgcgcaa catatactca aacacatatg gacttcatta ttgaagcctt taaacatgtg
     1621 aaagagaacg cggcgaatat taaagggtta acctttacct acgaaccgaa agtattgcgt
     1681 cacttcaccg caaaactgaa agaagtttaa ttaaaacact cagagtggct ataaggatgt
     1741 tagccactct attaccctac atcctcaata acaaaaatag ccttcctcta aaggtggcat
     1801 catgactgat caatcagaaa aaaaacactc tgcattttgg ggtgttatgg ttatagccgg
     1861 tacagtaatt ggcggtggga tgtttgcttt acctgttgac cttgccggag cctggttttt
     1921 ttggggcgct tttattctta ttatcgcctg gttttcaatg ttacattcag ggctattatt
     1981 attagaagca aacttaaatt atcccgtcgg atccagtttt aacaccatca ccaaagattt
     2041 aatcggtaac acctggaaca ttatcagcgg tattaccgtt gccttcgttc tctatatcct
     2101 cacttatgcc tatatctctg ctaatggtgc gatcattagt gaaacgatat caatgaattt
     2161 gggctatcac gctaatccac gtattgtcgg gatctgcact gccattttcg ttgccagcgt
     2221 attgtggata agttcgttag ccgccagtcg tattacctca ttgttccttg ggctgaagat
     2281 tatctccttt gtaattgttt ttggttcttt cttcttccag gtcgattact ccattctgcg
     2341 cgattccacc agcaccactg cggggacgtc ttacttcccg tatatcttta tggctttgcc
     2401 ggtgtgtctg gcgtcatttg gtttccacgg caatattccc agcctgatta tttgctatgg
     2461 aaaacgcaaa gataagctca tcaaaagcgt ggtcttcggt tcgctgctgg cgctggtgat
     2521 ttatctcttc tggctctatt gcacgatggg gaatatcccg cgagaaagct ttaaggcgat
     2581 aatctcctca ggcggcaacg ttgattcgct ggtgaaatcg ttcctcggca ccaaacagca
     2641 cgggattatc gagttttgcc tgctggtgtt ctccaactta gctgttgcca gttcgttctt
     2701 tggtgtcacg ctggggttgt tcgattatct ggcggatctg tttaagattg ataactccca
     2761 cggcgggcgt ttcaaaaccg tgctgttaac cttcctgcca cccgcgttgt tgtatctgat
     2821 cttcccgaac ggctttattt acgggatcgg cggtgccgga ctgtgcgcca ccatctgggc
     2881 ggtaataatt cctgctgtac tggcaatcaa agcccgcaag aagtttccca atcagatgtt
     2941 cacggtctgg ggcggaaatc ttattccggc gattgtcatt ctctttggca taaccgtaat
     3001 tttatgctgg ttcggtaacg tctttaacgt gttacctaaa tttggctaaa tccttcagga
     3061 agccagccat tcgctggctt cttgcctgtc aggaaatcac ttatgtccaa atggcaactc
     3121 gcctgattct ccttcaccac gtatgctttg cgtcacctta ctatcaggac gctttagccc
     3181 atgtcccgct ttttgatttg tagttttgcc ctggttttac tttatcccgc agggattgat
     3241 atgtacctcg ttggtttacc gcgcatcgcc gccgatctca atgccagcga agcgcagttg
     3301 catattgcgt tctccgttta tctggcgggg atggcggctg cgatgttatt tgccggtaaa
     3361 gtggccgatc gttcagggag aaagcctgtc gccatacccg gcgcggcgct atttattatc
     3421 gcctcggtgt tctgttcact ggctgaaacc agcgcgttat ttcttgcagg gcgatttcta
     3481 cagggactgg gcgcgggctg ttgttacgta gtggcgttcg ccattttgcg cgacacgctg
     3541 gatgatcgac gtcgggctaa agtgctgtcg ttactcaacg gcattacctg catcattccg
     3601 gtgttagcgc cggtgctcgg acacctgatt atgcttaaat tcccttggca gagtctgttc
     3661 tggacgatgg caacgatggg catcgcggta ctgatgttgt ctttgtttat tttaaaagaa
     3721 acgcgccctg cggcccccgc agcttcggac aaaccacgag aaaatagcga gtcactgctt
     3781 aaccggtttt tcctcagccg tgttgttatc accaccctca gcgtttcggt gatcctcacc
     3841 ttcgtcaaca cgtcgccagt attgctgatg gaaatcatgg gttttgagcg cggagaatac
     3901 gccaccatta tggcgttgac cgctggcgtc agcatgaccg tttcattctc cacgccgttt
     3961 gcgctgggaa tttttaagcc acgtacgttg atgatcacct cacaggtgtt attcctggcg
     4021 gcggggatca cccttgccgt ttcaccttcc catgcggttt cgctgtttgg tatcacgctg
     4081 atttgcgccg gtttctcggt aggttttggt gtggcgatga gtcaggcgtt agggccgttt
     4141 tcattacgcg cgggcgtagc cagctcgacc ttaggtattg cgcaagtttg cggttcgtca
     4201 ctgtggattt ggctggcggc ggtggttggt atcggcgcat ggaatatgct gatcgggatt
     4261 ctgattgcct gtagcatagt gagcctgttg ctgattatgt tcgtcgcgcc tggacgcccc
     4321 gttgccgctc atgaagaaat ccatcaccac gcttgatctc aatttgctgc tctgtctgca
     4381 actgctgatg caggagcgca gcgtgaccaa agcggcgaag cggatgagcg tgacgccttc
     4441 ggcggtgagt aagtcgctgg caaaacttag aatgtggttt gatgacccgc tctttgtgaa
     4501 ctcaccgctg ggcctgtctc ccacaccgct aatggtcagc atggagcaaa atctggcgga
     4561 atggatgcaa atgagcaatc agttgctgga taaaccgcac caccagacac cgcgcggtct
     4621 taagttcgaa ctggcggcgg aatcgccgct gatgatgatc atgcttaatg cgctgtcgaa
     4681 acggatctac caacgatacc cgcaggcgac catcaaatta cgtaactggg attacgattc
     4741 cttagatgcc attactcgtg gtgaagtgga tattggtttt tccggtcgcg aaagtcatcc
     4801 ccgttcgcgg gagctgttaa gctcgctacc gttagccatt gattatgaag tgctgtttag
     4861 tgatgtgccc tgcgtctggt tacgcaaaga tcatccggca ctgcatgaag catggaatct
     4921 ggacaccttt ttacgttatc cgcatatcag catttgctgg gaacagagcg atacctgggc
     4981 gctggacaat gtgttacagg agctggggcg cgaacgcacg attgctatga gcctgccgga
     5041 attcgagcag tcactgttta tggcagcaca gcccgacaat ctgctactgg cgaccgcgcc
     5101 gcgctactgc cagtactaca atcaactcca ccaactgccg ttggttgctc ttcctctccc
     5161 gtttgatgaa agccagcaaa aaaagctgga agtccctttt acccttctgt ggcataaacg
     5221 gaacagccgt aatccgaaga tcgtctggtt acgggaaacc attaaaaatc tttacgcgtc
     5281 gatggcataa ccgaatcgta tgaaacgcga cccaatttca caatgaaatg taaaaaagtt
     5341 gtaataaact tatctgaatc gaacttttag ccgctttagt ctgtccatca ttccagtaaa
     5401 tgattactct tgtattcata atggaccatt aagcatggag cgaaaaatgg cgactcactt
     5461 tgcccgaggg attttaacgg aaggacatct gatttctgtt cgtctcccct cccagtgtca
     5521 tcaagaagcc cgaaacattc ctcctcatcg tcaaagccgt tttctggcgt ccagaggttt
     5581 actcgcagaa ctgatgttca tgctgtatgg cattggcgaa ttgccggaaa tcgtcaccct
     5641 gccgaaagga aaaccggttt tcagcgataa aaatttgcct tcgttttcta tttcctatgc
     5701 cggaaatatg gtcggcgtgg cgttaacaac cgaaggtgaa tgtggcctcg atatggaact
     5761 acagcgtgcg acacgcgggt ttcatagccc acacgcgccc gataaccaca ccttttccag
     5821 caatgaatcg ctatggatca gtaaacaaaa cgatcctaac gaagcgcggg cgcagctcat
     5881 cacgctgcgc cgaagcgtgc taaaactaac cggtgatgtt ttgaatgacg atccgcgcga
     5941 tctgcaacta ctgcccattg ccggacgact gaaatgtgct catgtaaatc atgtagaagc
     6001 gttatgcgac gcggaggacg tgctggtgtg gtccgtggcg gtcacgccca cgattgaaaa
     6061 gctcagtgtc tgggagttag atggcaaaca cggctggaaa agcctgcccg atattcacag
     6121 ccgcgccaac aatcctacca gccggatgat gcgttttgcc cagctctcta ccgtgaaggc
     6181 tttttcgcca aattgataga caaccacagg agtcatcatg tctgaaaaat tgcaggtggt
     6241 tacgttactg gggagcctgc gcaaaggctc atttaatggc atggttgcac gtaccctgcc
     6301 gaaatttgct ccggcgagca tggaaatcaa tgcgttacct tccattgccg acattcccct
     6361 gtatgacgct gacgtacagc aggaagacgg ttttccagca acggttgaag ctctggcgga
     6421 acagatccgt caggctgacg gtgtggtgat cgtcacgccg gaatataact actcggtacc
     6481 gggtgggctg aaaaatgcca tcgactggct ttcccgcctg ccggatcaac cgctggctgg
     6541 taaaccggta ttgattcaga ccagctcaat gggcgtgatt ggcggcgcgc gctgtcagta
     6601 tcacctgcgc cagattctgg ttttcctcga tgcaatggtg atgaacaagc cggaatttat
     6661 gggcggcgtg attcagaaca aagttgatcc gcaaaccggg gaagtgattg atcagggtac
     6721 gctggaccac ctgaccgggc aattgaccgc atttggtgag tttattcagc gagttaagat
     6781 ctaaataaaa aacccgccag caatcatgca tggcgggttt ttaacgcgct atcgatttta
     6841 gtgagcgtcg ataaagacaa tcttcaggat aaacagcagc gcaacgatga ttacgcacgg
     6901 gctaaggtca cgcagacgtc cggtaccaat cttcatcacg cagtaggaga taaagcccag
     6961 cgcgatacct tcagtaatcg agaagctgaa cggcatcatc acagcggtaa taaacgccgg
     7021 aacagattca gtaagatcct gccagttcac gcgagcaaga cttgaggtca tcaacacgcc
     7081 aacgtaaatc agcgcaccag ctgcagcgta gcctggcacc atccccgcca gcggcgacag
     7141 gaagataacc agcaggaaca gcagaccaac aaccactgcc gtcagaccgg tacgaccgcc
     7201 aacggaaacg ccggaagaag attcaatata cgccgtaaca gaagaagtac cgataaacga
     7261 accggtcacc gaagagatac tgtcgacata cagcgcctgc ttcatgcgcg ggaatttacc
     7321 tttctcatcc gccagtcctg ctttatcggt cacgccaatc agtgtaccgg aggagtcaaa
     7381 caggttgacc agcatgaaag agaaaatcac ccctgccagc ccgaggttaa acgacccggc
     7441 taaatctaca tgacccacga cagtcattac gctcggcggc gcagaaacga tgccattgta
     7501 gtggacatca cccagtatcc agcccagcag cgtcgtcacc acgatggaaa ccagcaccgc
     7561 cgcgtgaatg ttgcgtgagg ccagaatagc aatgatgaag aagccgagga tccccagcag
     7621 tacgctgtga gaagtcagat taccgatgct caccagcgtt tccgggttag cgacaatcac
     7681 acctgcattc ttcagtccca tcatgccaat aaacaggccg ataccgctgg taatgcccac
     7741 acgcagactc accggaatgt tggctatcat ccagtagcga acgcggaaaa tcgtcagtaa
     7801 cagcagacca atcgcgcccc agaagattgc gcccatcccg acctgccacg gcaagcccat
     7861 cgcctgcaca accacaaaag cgaagaacgc attcaggccc attgccggtg ccagtgcaac
     7921 tggcaggtta gcaaacagcc ccatcataat actgccgaat gcagcaatca gacaggtcgt
     7981 gacgaagacg gcgctggtat ccatgccagc aacgccaaga atttgcgggt taacaaaaac
     8041 gatgtaaacc atcgtcagga aggtggtaaa accggcgatc acttcggtcc gtgccgtcgt
     8101 gccatgttcg cgcagtttaa acacgcgttc cagcatcccc tggccagaag cctgggttgt
     8161 gtgttgttga ctcattatct atttccgaac aaggagggaa aatccgtcgc tatcgtatac
     8221 caaaatgaga caatatggac ggctgaatat aacttttttc attgtatttg cttatacggc
     8281 aacgattgcg tcatgcgaat tggcatgacg aaaacgttaa actaacgata aaggaaagac
     8341 atgtcccaga tagaagcggt atttttcgac tgcgacggta cgctggtcga tagcgaggtc
     8401 atttgctctc gtgcctatgt cacgatgttc agggaatttg gcattcacgt tgacctggaa
     8461 gagattttta cccgttttaa aggcgtaaaa ctctacgaaa tcatcgacat catcagtaaa
     8521 gaacagggcg tgacgatggt ccaggccgat gccgagcacg tttaccgcgc agaagtagca
     8581 cgtctgtttg ataccgaact tgaggacatc gcaggagcga acgaattgct ggcaagcatt
     8641 aacgtgccga tgtgcgtcgt ctctaacggt ccggtcagca aaatgcagca ttcgcttggt
     8701 aagctcaaca tgctgcacta cttcccggaa aaactgttca gcggctatga tatccagcgt
     8761 tggaagcccg atccggcatt aatgttccat gcggcaaaag cgatgaatgt gaacgttgaa
     8821 aactgcattc tggtcgatga ctccagcgcc ggtgcgcaat cagggataga tgccggaatg
     8881 gaagtgtttt acttctgcgc tgacccgcac aataaaccga tcgttcaccc gaaagtcacc
     8941 acctttaccc atctttcgca attaccggaa ctgtggaagg cgcgtggttg ggatattacg
     9001 gcatagttat tcacactccc ttcacttact ccacttaaat tggcacgcaa aggtaagtaa
     9061 agggagtttg atatgtctgt ttcacgtcgg gtaattcatc acggactgta ttttgcggtt
     9121 ttagggccgt taattggtgt tctgtttctt gtcctctaca tattcttcgc aaaagaaccg
     9181 ctgattcttt tggtaataat acaggtactt ccattgtttc tcttaatgtc gataactacg
     9241 ggagctattc ctgcaatgtt aactggcgta atggtcgcct gtctgccgga aaagatcggg
     9301 tcacagaaac gttatcgttg tctggttggc ggcataggtg gagtcgttat caccgagatc
     9361 tattgcgctg ttatcgtaca tattaaggac atggcttcct cagcgttatt tgaaaacatt
     9421 ctttctggtg aaaatctcgt tgtccgcatc atcccagcat tgctggcagg cgtgttgatg
     9481 agcagaatca ttacacgtct acccggattg gatatttcat gtcctgaaac agactcttta
     9541 agctaagcgg aatgttttat ctatttgtca ttaaatgttg gagaacaggc cggagcgtaa
     9601 ttcacacatc cggccttatt tttaagctta accgagcgct aattctgcgg cagcggcttt
     9661 atcagcgatt accatcagcg aaggatgcag ctgcaacacg gaagctggaa catcttcggt
     9721 aaccggacct tgcagaacat ttttcagtgc ctgcgcttta cctgcgccac tgacaattat
     9781 cagcaagttt ttcgccgcca tgatgctttt cggccccatc gtcacgtagc tgtcaggcac
     9841 taatgaaaag tcgccgccca actcgccatg cgccacaata tcaaccatct ctccctggat
     9901 cgggaactcc acggtttgct catggaaatg ggtcgtattt ggcagattgc cacaaaaatg
     9961 accatctaca cctaatccca acaccaccaa atccaggccg ccttcacgcg ccagtttctg
    10021 atcatgctcg cggtagttat caatagtgag cttctggata ttctcttctt tgatccccgc
    10081 aggggtgaaa aacagattac gcagattggt aatcgttacg ccttctccct ctttgccgcg
    10141 aaatggaatt tcatcgaaat tatagaaata gcagttatcg taccagggct tacctttaac
    10201 cagagtgatg agatattcgt acatgccttt gggcgtgctc ccggcggtaa ttgccaggtt
    10261 aacacgacgc gtcttcgaca tataaccaag cagatggtgt gccgcgacac ggctcatttc
    10321 ctggtaatct tcggtaatga ttaatttcat cttaatgcct tatattattt gaataatttc
    10381 tgtacgaagg ctgcataggc aggccgccag acatccattt catggttcag acccggatat
    10441 tcctggtaat caaacttaat ttttttctgc tcaagctcag ttttcagccc ggcgatatcc
    10501 ttgccggtta caacatcttt atcccccaca accacagtaa aattacgtag ttgctggtta
    10561 atagctgccg gatcgttcag ccgggcagcg accccttcat ctggtacggt cgtcgtggta
    10621 acaccactga atgtggccag ccagccaaag ctttccagat gattcattcc cgataccagt
    10681 gcctgatacc caccttgtga aagccctgcc agtgcgcgac catcggcatc tttacggaca
    10741 ttaaaacgct tgctaatcag cgggataata tcgttcatca gttcgcgatc tgccgcttta
    10801 gcatttaacg gataaaagac tttacgtctc tcctgaggaa cgaaatcttc gggaataatg
    10861 cccttcgcat cggtttcggt atccgggatc accaccagca tcggtttaat tttcccttca
    10921 gccagcaggt tatccatgat ttgcgggata cgcccctgat caatagcgga acgtccggta
    10981 tcaccaaagc cgtgatagaa atagagcact ggcaaaggct cgcctatgcc ggtgtatcct
    11041 ggcggggtcc agacatacat ctgacgttcg gattgcaatg cgttggagtg gtaagttatg
    11101 gcgatcaaat caccatgcac aacagaacgt gtatccagat aactgcccgg taccagaatc
    11161 atactggagt taacctgacg ctgaggctta gtcattgccg tgcctgtatc aatgctgcgt
    11221 acaccatcaa cattgaaaaa atactcgtac agattgcctt tcaggacagg cgtgcgccac
    11281 gaccagactc ccgcttcatc tttgtcatcg ggtgaatatt gtccggaacc ggaacaccca
    11341 ccactacgga gacatttttt gccccagggg caaagtagcg aaaggtcacg ctgttatcgg
    11401 tattgacctg agtcacatac tgtttcaccg gaatagtggg tgccgggctg gctggcatat
    11461 ccgctgctat ggcccattga gcagaaatac cgctggcaat agccagcgtt aaagcagcaa
    11521 tttttatatt catcgtaata ttccttttat taagttccat taccaccaga tttcagcctg
    11581 ggcaccgaca gcaaactggt cgtctttatt gtcttcaaag gtgaagccat ccagttcgtt
    11641 ttccagagct ttgatatatg tagcgtagaa acgaatttcc gggcgcgagg tcaacatact
    11701 ggtattgact ttaaaggtat ggaagagcgt ggttttataa ccagactcat tatatttatt
    11761 gctattcgca tctttgtttt gctgggtgaa atagcccagt tcaacacctg tctggttata
    11821 ttggtcccaa atataggccg ggcgaacaac cgcacgaata gattcgaaat cagaatgtgc
    11881 gcctgtttcg tagctataaa tatcgttacc gaaggagtaa acaatcgcgt tagccacaat
    11941 gaaatgatcg ccgatataag cttcgccctg cgaagtcaga cgaactgcag ttccgcctgt
    12001 gtgatcacca taataacgac cattaaaggt agtaaatgga cttgcaccag cataacgacc
    12061 aaagttactg gcgatagagt tattcgcaac caggaaggag aattcgttga agccaccctt
    12121 atcaaatttc tgcgttaaag atgtaccaaa catccaggta tctttccagt cataataccc
    12181 gttattatcc tgattatctt tttccgatgc gctttcattt gctgagacat aacgaccgct
    12241 caccattaag gtggctttat cccataacgg gatatcttta tagcgtaaat caatggtatt
    12301 ggtattaatc tgctgtttgt tttgcaggct gcgatcgtaa tcatcgacat cttcacgaac
    12361 cagcgcgata tcaattttac ccggaccgac tttccagttt tccagaccta cacctgcggc
    12421 agcatcggta cgctgcgttt tccagtcaag catctggatt tcaattttcg gcgcaccgtg
    12481 tttacccacc cagaaatcag cctctggcgc aaagggcagg aaacctttgg tggtaacata
    12541 catatcagag aactgcataa agttctcgcc accggcgtta tcgccaaacc agccagtaga
    12601 gtactgctga cccacgttac catccatcat cacgatggca tcaacccgtt tgccgttttc
    12661 gttgtagaca cgttgtttta actgcaaatc aaaccagccg gagtattcgt taccaaagcg
    12721 gcccagagaa ccaattgccc atgatttagg tgaaccatga gaggctgttc cccaaccaga
    12781 acggtagtag ccgttgtaac taaagccgat ttcatctttc acaaatttac tgaaatcttt
    12841 cagagtcatg gaagaataaa tcgagcctgt ctcactggca tttttctctt catttttaac
    12901 cagtacagca tcaggctttg cggcacgact ggtcgggaat ggattggcgg catacccttg
    12961 atcattcgta cttacgctac gattcaccgt cgctggcgta tactttttct tctcttcatc
    13021 tttatatttt ttcagttcgc tttgcgtttc tcttaacgcc ttttctaata gctcaaggcg
    13081 ttgttccact gttaatgatt cagccaatga ttgtgcggaa aaggctaacg gtgccattaa
    13141 taagatggca gaggtaataa tatttcgtct aaacataata tccctttatg gtgcaaagaa
    13201 agaattaacg catcgcatcc agactgttct gaatgcgacg atatttaagg tgctttattg
    13261 gttatttttt taatgatagc ccccgagttt tgatcacttc tgcataccat ccgaagcttt
    13321 tcttgcgcgt tcttgtgagg cttccttcac cattatcatc acgatccaca taaataaagc
    13381 cgtagcgctt agacatttgt gaatgagatg cactgactaa atcaattggc ccccaactgg
    13441 tgtaccccat aatatccaca ccatcggcaa tcgcttcatt tacctgtacc aggtgatcgt
    13501 ttaaataggc aattcgataa tcgtcctgta tcgaaccatc cgcttcaacg ctgtcttttg
    13561 cgcctaatcc gttctcgaca ataaataacg gtttttgata acgatcccaa agtgtattta
    13621 acagaacccg taatccaacc ggatcaattt gccaccccca ctctgaactt ttcagatgcg
    13681 gattggggat catattcagt atgttgccct gcgcattttt attaatgctt tcgtcgtggg
    13741 aaacacaacc ggtcatgtaa taactaaaag agatgaaatc tacggtatgt tttaaatctt
    13801 ctgcgtcact ttcagtcatc tcaatggtga tattgtggtc gcggaagaaa cgctgcatat
    13861 agccgggata ctgaccacgc gcctgaacat caccaaagaa catccagcgc cggttctctt
    13921 ccatggcctg caacatatcc tgtggctggc aggtgagggg gtaaaccagc ccaccgagaa
    13981 gcatattgcc aattttcgct tcggggatca ggctatgaca ggctttaact gcccgcgcac
    14041 tggcaaccag ttgatgatgg atcgcctgat aaacttccgc ctcgccactc tcttctgcca
    14101 gccccacgcc cgtgaatggc gcgtgtaatg acatgttgat ttcattaaat gtcagccata
    14161 acgcaacttt atgttggtag cgggtaaaga ccgtgcgtgc gtaatgctcg aagtgatcga
    14221 tgaccgcccg attagcccaa ccgccgtagt ttttcaccag cccatatggc atttcgtaat
    14281 gggataacgt taccagcggc ttgatccccg cctgcgccat ttcatcaaac agccgctcgt
    14341 aaaacgctaa ccccgcttca ttcggttcgg cttcatcgcc ctgaggaaaa attcgcgccc
    14401 aggcaatgga aatacgcaga caggtgaagc ccatctcggc aaataacgcg atatcttccg
    14461 ggtaacggtg ataaaaatcg atggcgacat ctttgatatt ctctttcccc aggatgcgcg
    14521 gttccatttt tcccattacg ccatgaggct gtaaatctga ggtcgtgatc cctttgccat
    14581 cttcctgcca ggcgccttcc acctgattgg cagctgttgc accaccccaa agaaatgttt
    14641 ctggaaatgc tttcataatt aactcctttt atcgttagcg aatgatggat aacagcggct
    14701 caccagcgtt tatctgcgcc gtgccgtggg gtaatacgtc cgtaaaatca tcgctattac
    14761 tgattaatac cggcgtcgtc agatcaaatc cggcctcgcg aatagcaggg atatcaaaag
    14821 aaatcagccg atcgcctgta ttgaccttgt cacccacatt gacgtgagcg gaaaagaatt
    14881 tgccgtccag ttttacggtg tcgataccga catgaatcag gatctccacg ccatcatctg
    14941 actcaatgcc gatggcatgt aatgtggcga acaacgaagc aattcgaccc gccaccggag
    15001 aacgcacttc accaacagag ggcagaatgg caataccttt acccaacagg ccgctggcaa
    15061 acgtggtatc agcgacgtga atgagcggca caatctctcc cgtcatcggt gaacagatac
    15121 cgccctgctc aggtgtaata acctctggtg ttttctcttg ctgggcaact tgcgctgact
    15181 gactcttagc ggtgatgaaa tgaaacatca ccgtaccgac aaatgcgcaa ccgatggcaa
    15241 tgacaccgcc aataacgctg gcccatacgg tgaaatcaat ccccgttgac gggatggttt
    15301 gcatgaaggt gaaaatacct ggcaaaccaa aggagtaaac tttcgtttgc gcgtagccaa
    15361 tgatggtggc ccccaatgcc ccacttatgc aggcgataac gaaggggtac ttacgcggca
    15421 gattgacgcc atataccgct ggttcggtga taccaaacaa acctgtcaac gccgctgatc
    15481 ccgccaccac ttttttctgt gcatcgcgtt cgcagaggaa gacgccgagc gccgccccga
    15541 cttgcgccat aatggcgggc attaacagcg ggatcatggt gtcgtagccc agcacggtga
    15601 agttattgat aattaaaggt atcaaacccc aatgcagacc gaacatcaca aagatttgcc
    15661 agaagccgcc cattaccgcg cctgcaaatg caggaaccgc ctgataaagc cagagataac
    15721 cggcagcaat cagttcactt atccaggttg atagcggccc caccagcaga aatgtgatgg
    15781 gcgtgatgac catcagacat agcaagggtg tgaagaaatt tttgattgcc gaaggtaacc
    15841 acgcattaag tcggcgttcc agaatgctgc acaaccaggc agaaaaaata atgggaataa
    15901 ccgatgacga gtaattcaac aatgtgaccg gaatacccag gaaatccagc cccagcgcat
    15961 ccactttttg cccgttctcg aaggcagtca gaattaatgg atgcactaac gctccgccaa
    16021 tcaccatggc agtaaatgga ttgccgccga agcgtttccc tgcggtgtag cccaggatta
    16081 tcgggaagaa ccaaaacaag gcatcactgg cgctaaataa aattaaataa gtaccacttt
    16141 gttcggtcgc ccactgaaaa gtgagtgcca gagccaacat acctttcagg atcccggttg
    16201 ccgccatcaa accgatcaga ggcgtaaaaa tacctgaaat aacataaaca aagcggttta
    16261 acagattatc tttatcatca ttttccggtg cctgttgcgc tttttcgcca aggcctgcca
    16321 cgctgttaac cgccaggaag acatcggcca catggttacc tatgacaacc tgaaactggc
    16381 caccgctttc caccaccata ataataccgg gggtcttttt cagtacctca gcttgcgctt
    16441 tgctttcatc ctttaactta aaacgtaatc gcgttgcaca atgcatcaga ctcacaatgt
    16501 tatctgcgcc cccgactcct gcgactattg ttctggctaa ctccgtcata acttgccctc
    16561 taccgctttg cggcaaaact ccaaaaaaaa acctgaaaaa aacggcctga cgtgaatcaa
    16621 gcaatttttt tcaggttttg cccgcttagt gcggtaacaa tcctttattc agtaataata
    16681 tttcagtgtt ctttgcgcac gcgctctata tttatggcta aaaacataat ctctgcaggt
    16741 gagattttac gttgatactg caaaccaata aaaatagcga tccgctccgc acattgccat
    16801 gcttgcgggt aattttgctt tactgcttgt tgtaatgatt catcactatc gttaatcgat
    16861 gcatgttcaa taatacgcca ggataaaaat ttcagatgcg taaccagtcg ctgataactc
    16921 aagctttctt cctggtaatt aaggctgaac tgaaatttta ttaattgcag catttctcgc
    16981 attaattgcg tgacacctgc aacatcctcc atatttccgc tcatttgggc actgaccaga
    17041 tgcatggcaa taaagcccac ttcatctttc ggtaactgca cgcccaaccg tttatcaata
    17101 atggttaacg cttcttcccc tagctggaac tctttcgggt aaagtcgctg gatatcccac
    17161 agcagcgggt tgggcagtag cacgttttgc tgaaagcgtt taatcgcaaa ctggcaatgg
    17221 tcagttagcg agatataaat actgtcctgc aactttccca gacgctcctg cgccagagag
    17281 ataatacgat cacaggttgc catcacctca agaggcatat gacttaagag ttcgcttaat
    17341 cgcccgttca gttcatgact gctcaaggca tactcttttt ctattccact tgagttaatt
    17401 ctttcgcctg ggcgtttttg aaagccaatt ccgcgcccca tgacgacttt ttcccgctgt
    17461 tgatcatcaa taaccaccac aacattattg ttgagaattt tggtgatttg catgttcata
    17521 gcaaggacct tttttataaa caaaaaaacc cgacttcacc agtattctct ggttatgtca
    17581 ggttttgcct gcgaatgcag taacaatcca gtcattaatt aatggttttt ataacgaaca
    17641 tccaggttcg aaaatta
//
LOCUS       DADSYM010000105        17374 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2415
            SAMN08039579-rid11723173.denovo.105, whole genome shotgun sequence.
ACCESSION   DADSYM010000105 DADSYM010000000
VERSION     DADSYM010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039579
            Sequence Read Archive: SRR6362533
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17374)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17374)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980162.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 03:39:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,484
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 4,347
            CDSs (with protein)               :: 4,347
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 43 of 85
            Pseudo Genes (incomplete)         :: 37 of 85
            Pseudo Genes (internal stop)      :: 19 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17374
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039579-rid11723173.denovo.105"
                     /strain="LGE2415"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8302 N 95.9628 W"
                     /collection_date="2015-08-24"
     gene            31..549
                     /locus_tag="J1Y83_003044"
     CDS             31..549
                     /locus_tag="J1Y83_003044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HBA7631172.1"
                     /translation="MMKFKKCILPVAMLASFTLAGCQSNADDHAADVYQTDQLNTKQE
                     TKTVNIISILPAKVAVDNSQNKRNAQAFGALIGAVAGGVIGHNVGSGSNSGTTAGAVG
                     GGAVGAAAGSMVNDKTLVEGVSLTYKEGTKVYTSTQVGKECQFTTGLAVVITTTYNET
                     RIQPNTKCPEKS"
     gene            565..1104
                     /locus_tag="J1Y83_003045"
     CDS             565..1104
                     /locus_tag="J1Y83_003045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA7631173.1"
                     /translation="MKKVFLCAILASLSYPAIASSLQDQLSAVAEAEQQGKNEEQRQH
                     DEWVAERNREIQQEKQRRANAQAAANKRAATAAANKKARQDKLDAEATADKKRDQSYE
                     DELRSLEIQKQKLALAKEEARVKRENEFIDQELKHKAAQTDVVQSEADANRNMTEGGR
                     DLMKSVGKAEENKSDSWFN"
     gene            complement(1197..2774)
                     /gene="guaA"
                     /locus_tag="J1Y83_003046"
     CDS             complement(1197..2774)
                     /gene="guaA"
                     /locus_tag="J1Y83_003046"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HBA7631174.1"
                     /translation="MTENIHKHRILILDFGSQYTQLVARRVRELGVYCELWAWDVTEA
                     QIRDFNPSGIILSGGPESTTEENSPRAPQYVFEAGVPVFGVCYGMQTMAMQLGGHVEA
                     SNEREFGYAQVEVVNDSALVRGIEDALTADGKPLLDVWMSHGDKVTAIPSDFVTVAST
                     ESCPFAIMANEEKRFYGVQFHPEVTHTRQGMRMLERFVRDICQCEALWTPAKIIDDAV
                     ARIREQVGDDKVILGLSGGVDSSVTAMLLHRAIGKNLTCVFVDNGLLRLNEAEQVLDM
                     FGDHFGLNIVHVPAEDRFLSALAGENDPEAKRKIIGRVFVEVFDEEALKLEDVKWLAQ
                     GTIYPDVIESAASATGKAHVIKSHHNVGGLPKEMKMGLVEPLKELFKDEVRKIGLELG
                     LPYDMLYRHPFPGPGLGVRVLGEVKKEYCDLLRRADAIFIEELRKADLYDKVSQAFTV
                     FLPVRSVGVMGDGRKYDWVVSLRAVETIDFMTAHWAHLPYDFLGRVSNRIINEVNGIS
                     RVVYDISGKPPATIEWE"
     gene            complement(2843..4309)
                     /gene="guaB"
                     /locus_tag="J1Y83_003047"
     CDS             complement(2843..4309)
                     /gene="guaB"
                     /locus_tag="J1Y83_003047"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004159092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HBA7631175.1"
                     /translation="MLRIAKEALTFDDVLLVPAHSTVLPNTADLSTQLTKTIRLNIPM
                     LSAAMDTVTEARLAIALAQEGGIGFIHKNMSIERQAEEVRRVKKHESGVVTDPQTVLP
                     TTTLREVKELTERNGFAGYPVVTEENELVGIITGRDVRFVTDLSQPVSVYMTPKERLV
                     TVREGEAREVVLAKMHEKRVEKALVVDDEFHLIGMITVKDFQKAERKPNACKDEQGRL
                     RVGAAVGAGAGNEERVDALVAAGVDVLLIDSSHGHSEGVLQRIRETRAKYPDLQIIGG
                     NVATAAGARALAEAGCSAVKVGIGPGSICTTRIVTGVGVPQITAVADAVEALEGTGIP
                     VIADGGIRFSGDIAKAIAAGASAVMVGSMLAGTEESPGEIELYQGRSYKSYRGMGSLG
                     AMSKGSSDRYFQSDNAADKLVPEGIEGRVAYKGRLKEIIHQQMGGLRSCMGLTGCGTI
                     DELRTKAEFVRISGAGIQESHVHDVTITKESPNYRLGS"
     gene            4471..5841
                     /gene="xseA"
                     /locus_tag="J1Y83_003048"
     CDS             4471..5841
                     /gene="xseA"
                     /locus_tag="J1Y83_003048"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000937953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HBA7631176.1"
                     /translation="MLPSQSPAIFTVSRLNQTVRLLLEHEMGQVWISGEISNFTQPAS
                     GHWYFTLKDDTAQVRCAMFRNSNRRVTFRPQHGQQVLVRANITLYEPRGDYQIIVESM
                     QPAGEGLLQQKYEQLKAKLQAEGLFDLQYKKSLPSPAHCVGVITSKTGAALHDILHVL
                     KRRDPSLPVIIYPTAVQGDDAPGQIVRAIELANQRNECDVLIVGRGGGSLEDLWSFND
                     ERVARAIFASRIPVVSAVGHETDVTIADFVADLRAPTPSAAAEVVSRNQQELLRQVQS
                     ARQRLEMAMDYYLANRTRRFTQIHHRLQQQHPQLRLARQQTMLERLQKRMSFALENQL
                     KRAGQQQQRLTQRLNQQNPQPRIHRAQTRIQQLEYRLAETLRAQLSATRERFGNAVTH
                     LEAVSPLSTLARGYSVTSAADGAVLKQVKQVKAGDTLTTRLNDGVVVSVVSDVAKTRK
                     PRKKNS"
     gene            complement(5880..13922)
                     /locus_tag="J1Y83_003049"
     CDS             complement(5880..13922)
                     /locus_tag="J1Y83_003049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001535498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RatA-like protein"
                     /protein_id="HBA7631177.1"
                     /translation="MSLTLNKILVLCALLISAMLPGWSWAESAWQDSSDTVGEFNGTV
                     PTADSASIPVYQGSVFLDPAKTHDVAFTAKPSEFSADVSVSKLLVTNPQDREGDIIAT
                     PRWENQTPPAISLVWADAATPGTLLDPQPVADRSFCAQGLAGRSLVAWAQPDPQQTMP
                     LLYLLTSTGYPYESVLTLADQKVTLKIAPAQGDLISVSAAGYDESSGAAKMTVGGSIT
                     LTVTTKDCVGNVVGNIPFVIKRKDAENRQGVVNNTAPVKLGTTELTTTATEYRGTTDA
                     NGVATVTVTQANGPGVKTPLVASLAGIAQASETAVIFTVLTSPDVPQATMWGHMPDTL
                     KARDYTFSRPKLAAEVDNEGGTVNDHNETWSTFTWSGADKHCDILPGMRQFGALATVV
                     PTSVQDVAGWPMQGNFYWSSLAGMSGQHHAADVSNRSEAQKPDDTTFIVSCVDKEAPD
                     VEPKLVLTPGSYDSTIKAMKVKVGEEASLRLTITDSKNNDQPLAYYYFSLHLDDGINR
                     KNQTDAAWETHPVQIDGGSNVRKVDAHTYEGITDANGEATLTLTQPGGVGVKTHITAR
                     MRSDFTASDEKDVIFTVITSPDTDKARMWGHMLGIIEANNIFKRPRLADETDNELGSV
                     RENNEDWALFDQNSSMQAECGLGHIPSQSSLHSLFAAHPANAIGTEYGWPTLQKAYLS
                     AVEETSHASVNLATGNIDTYSGFKQNYLSCSGNEMVAKIAATTDRDVSAGSRAQAKVG
                     DTITMTVRTFNALNNAPVPYTAFTITKDMGKNRQGQTTGFDDPTRGAIEMNGTLYGTS
                     QPSLVYAGTTDAQGFATVEIKQSQGVGLSTPLNIVPVNSYIPNTVNYNVIFTTLTSPD
                     AVGAQMWGHMDETITVDALTFARPRLAAEVFSPDGTLTENNEVWSRVSQANASSTSKG
                     GCGANMLPRRSQLSALYDANNGDGVQTVHGWPTQRQPYWSSSPADQVPHYYTIALNDG
                     ARTVGGSTAVYVSCLTTANNPASSITLEVVDPAQWNAAANAAKLKKGETLQVKVTVKD
                     AQGNPLGDIPFTLKRGDGYTRSEEKHVAGSGDALVVVNGGLADETSLNNTAAAYSAIT
                     GSDGTKILTITRPDTHGTKTSLTATLYSDTTKKATLDTIFTVVTSPDSDKAKMWGHMP
                     ETVTAADGAVFKRPLLLKELSSTSGRTAIAEENEDWAQFTQTQAISTSSNGCGSEYVP
                     SQAGLESLYEANRGNAMKTVQGWPVASSYLSSTTGSSSLEQRDFKAVNLSSGTSSIIP
                     SATKELLTCQTTPIVKASQIVLEAADPAKFDSTNNVVKAKKGEEIVVRVTTKDSQGNP
                     VGNTAFTLKRNTSVNRANVSITTSIASLAVTDAWGNTQNDFLSTTLVIYGVTGADGTT
                     TFTLKQDQTTGLKTELTAALDSSSSTKSTLPVVFTVLTSPDSPKAKFWGHMAETATGD
                     DGLIYRRPLLRDENSATTSIGTLVEEGEAWSTFPSGQANDTSINGCGAEYVPTDNELR
                     AIYAHQGSSALHDAIGWPVSRFYISNTVADTFTQTFTYDVVSLKTGDETQMPSSGGAL
                     LSCRTTPVAVASQIIVEANDTAQFVKVDDTLSALKVKKGEDAVIRVVTKNAQGNSVPN
                     VPFILRREGSKNRQYAEMINKSITVINAAGASARMNSSSSLLYGVTGADGTTSFTVKQ
                     DDSMGLVTNMYAQLYQPTIESNKLPVMFTVITSPDTPLASYWGHMPETFTTRSGIAFK
                     RPLLTAEHPAGQSTLANNESWLSLNTAAKNDVSKSDCGEPYQPLLSEFQELYSEHPNG
                     AIGTDLGLPLTNTWWAYDKIAYANVWYDQSINLSNGSSSRALSNTVAFVSCLVNPHAV
                     ADSIEMTSTALDAEKTASNDGRPSATAAKGTAIRMTVIVRDSAGNLLPGANFNLIRGT
                     ALDRAKNRLDSTYDDLTIVPVTPAGVNMSLYNNGAQALLTTGSDGKATFDVTQNETYG
                     LATPLTATLMRDTTKSATMDVIFTVITSPNSPKAKYWGHMPDTFTSRAGVTFKRPLLA
                     AEATLGSSVSNNNESWSYLFYTNKVTPDCPVEYQPRLNELQGLYNDHPGGTILTDLGL
                     PITAGSGNWWTYEMSTTDALTWYYGVINLKTGQSTTTINGYALMLCLTQPHSAPASLT
                     LSSTAYDEGRTASNGGTPTSSVKKGEMLPIVVTIKDANGNPVGGEGVTLKRVQAKSRS
                     GISVSSNTVDDLILDEVTPTSARISFNQNTSAWSGFTGSDGTITFNVTQNNTVGLVTP
                     FTASLARNPQVTANQDLIFTVVTSPDSAKANYWGHMPATLTAVNGAVFERPKLWSELT
                     STSGVGKINNNNEDWPYFTPTQKSDASVSPCEVARQPLFNDLSSLSARYPNNTFVTET
                     GWPAYYTWWAEDKSADGKDQSVDLRNGTLYTGSTKSFQPCLANARSTVSSVTLTSTAF
                     DADSQAAKVKKGEAMSVTVTVKDSAGNTVPNVEFTLKRGEASPRNAGATLYGNVVAMD
                     DLIVQPLSGSAITLSESGNTISGMTGADGTASFTLRQDNTPGYKTPLTVTLANYASAT
                     DTLDAIFTVPTSPNVSSAHFWGHMADTVVVNGKSLHRPLLTTELPSGANPVSSPIINY
                     ENWASAHIIDASKWDIARQCGSIENAPTYNELELLHTVFNSLGWPSSPSFPYLSSQQC
                     GMDEGTGAQDCSITLINKPGLVTCFQ"
     gene            complement(14043..15020)
                     /locus_tag="J1Y83_003050"
     CDS             complement(14043..15020)
                     /locus_tag="J1Y83_003050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019841502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA7631178.1"
                     /translation="MKQDKRRGLTRIALALALAGYCVAPVALAEDSAWVDSGETNIFQ
                     GTIPWLYSEGGSATTDADRVTLTSDLKGARPQGSETDKRLYSGDKLTVSWEIGDTEGD
                     VDLGGLGDNAKTIDTIRWMSYKDAQGGDPKELATKVTSYTLTDADRGRYIGIEITPTT
                     QTGTPNVGTALHLYDVSTASGGGSDSDNVAPGPVVNQNLKVAIFVDGTSINLINGSTP
                     IELGKTYVAKLYSDENKNGKFDAGTDADVTANYDFRWVLSGSSQQLGTSGGIVNSSFD
                     NNNLVIPATNDEARTNLNGPARDGKEALSIPTNGDGVQGYKLHIIYKHK"
     gene            complement(15075..17252)
                     /gene="sinH"
                     /locus_tag="J1Y83_003051"
     CDS             complement(15075..17252)
                     /gene="sinH"
                     /locus_tag="J1Y83_003051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001317981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intimin-like inverse autotransporter SinH"
                     /protein_id="HBA7631179.1"
                     /translation="MLRWKRCIILTFISGAAFAAPEINVKQNESLPDLGSQAAQQDEQ
                     TNKGKSLKERGADYVINSATQGFENLTPEALESQARSYLQSQITSTAQSYIEDTLSPY
                     GKVRSNLSIGQGGDLDGSSIDYFVPWYDNQTTVYFSQFSAQRKEDRTIGNIGLGVRYN
                     FDKYLLGGNIFYDYDFTRGHRRLGLGAEAWTDYLKFSGNYYHPLSDWKDSEDFDFYEE
                     RPARGWDIRAEAWLPAYPQLGGKIVFEQYYGNEVALFGTDSLEKDPFAVTLGVKYQPV
                     PLIVVGTDFKAGTGDNTDLSVNATLNYQFGVPLKDQLDPDKVSAAHSLMGSRHDFVER
                     NNFIVLEYKEKDPLYVTLWLKADVTNEHPECVIKDTPEEAVGLEKCKWTINALINHHY
                     KIVAASWQAKNNAARTLVMPVIKENTLTEGNNNHWNLVLPAWQYSSDQAEQEKLNTWR
                     VRLALEDEKGNRQNSGVVEITVQQDRKIELIVNNIANVPEENNHSHEASAQADGVDGV
                     VMDLDVTDSFGDNTDRNGGALPEDNLTPQLYDAQDKRVTLTNKPCSTDNPCVFIAKQD
                     KEKGTVTLSSTLPGTYRWKAKAAPYDDSNYVDVTFLGAEIGGLNAFIYRVGAAKPSNL
                     IGKDKEPLPLNNIYRFVLWRDNNKDGVFQQVEKLTDEEMAQYDYKWEFTGKSINGEVG
                     AQANTSNEDIVIPATNREAAQTYGAQAGDGLQGYGLRVLYTKK"
BASE COUNT         4101 a         4600 c         4325 g         4348 t
ORIGIN      
        1 tatttttcgt aatgtgtata acaagtaata gtgatgaaat ttaaaaaatg tattctgcct
       61 gtggcaatgt tagcgtcatt cactctggca ggatgccagt caaatgctga tgatcatgcc
      121 gccgatgttt atcaaaccga tcaactgaat accaaacaag aaactaaaac cgttaatatt
      181 atttccattc ttcccgcaaa agttgccgta gacaactccc aaaataaacg aaatgcacaa
      241 gccttcggcg cgcttattgg tgcagtcgct ggcggtgtaa tcggccacaa cgtggggtct
      301 ggcagcaatt ccggaacgac ggcaggtgca gttggcggcg gagctgttgg cgcggcagcg
      361 ggttctatgg tgaatgataa aaccttagtg gaaggtgttt ctctaacgta taaggaaggc
      421 accaaagtgt atacctccac ccaggtgggt aaagagtgcc agtttacgac aggtttagcc
      481 gttgttatta ccacgacgta taacgaaacg cgtattcagc caaataccaa atgtcctgaa
      541 aagagctaat aatcaggagg agtcatgaag aaagtttttc tttgcgccat cttagcctcc
      601 ttaagctatc cggctatcgc ctcatcattg caggatcaac tctcggctgt cgcagaagcg
      661 gaacagcaag gtaaaaatga agagcaaagg cagcatgacg aatgggtcgc ggagcgcaac
      721 agggaaatcc agcaagagaa gcaacgtcgc gcaaacgccc aggccgcggc taataaaaga
      781 gcggcaacgg cagcagcgaa taagaaagct cgtcaggata aactggacgc cgaagccact
      841 gcggacaaaa aacgcgatca aagttatgaa gatgagctac gcagcttaga gattcagaaa
      901 caaaaactgg cgctggcgaa agaagaagcc cgcgttaagc gagaaaacga atttatcgat
      961 caggaactga agcacaaagc tgcgcaaacc gatgtggtgc aatctgaagc tgacgcaaac
     1021 aggaatatga ctgaaggcgg tcgcgatctg atgaaaagcg tgggcaaagc agaagagaat
     1081 aaatcagata gctggtttaa ttaatcgatg ttagtaactt caatcctatg attcgtgagt
     1141 ataaaaaacc ctctgtagta acagagggtt ttgttcattc atagtgcaga gtcaaatcat
     1201 tcccactcaa tagtcgctgg tggcttgccg ctgatgtcat acaccacgcg ggaaatacca
     1261 ttcacttcat tgataatgcg gttggaaacg cgaccgagga aatcgtacgg cagatgtgcc
     1321 cagtgtgcgg tcataaagtc gatggtttcg acagcacgca gagagacaac ccagtcatac
     1381 ttacgaccat cgcccattac gccaacggaa cgtactggca ggaagacggt gaacgcctgg
     1441 ctgactttgt cgtacaggtc cgctttacgc agttcttcaa tgaagatggc gtcagcacgg
     1501 cgcagcaggt cacagtactc tttcttcact tcacccagaa cacgaacgcc gaggcctggt
     1561 cccgggaacg ggtgacggta gagcatgtcg tacggcaggc ccagctccag accaatctta
     1621 cgcacttcgt ctttgaacag ctctttcagc ggttcaacca gccccatctt catctctttc
     1681 ggcaggccgc ccacgttgtg gtgagatttg atgacgtgcg ctttaccggt tgcagacgcc
     1741 gcagattcga taacgtcagg gtagatggtg ccctgcgcca gccacttcac gtcttccagt
     1801 ttcagcgctt cttcatcgaa tacttcaacg aaaacgcgac cgatgatttt acgttttgct
     1861 tccggatcat tttcgccagc cagcgctgac aagaagcgat cttctgccgg tacgtgaaca
     1921 atattcagac caaagtgatc gccaaacata tccagaacct gctctgcttc gttgagacgc
     1981 agcaggccgt tgtcgacgaa tacgcaagtc aggtttttac cgatagcgcg gtgcaacagc
     2041 attgcggtta cggaggaatc cacgccacca gagaggccga ggatgacttt atcgtcgcct
     2101 acctgctcgc ggatgcgagc tacagcgtcg tcgataattt tcgctggcgt ccacagggct
     2161 tcacactggc agatatcacg cacaaaacgc tccagcatgc gcataccctg gcgggtgtga
     2221 gtcacttccg ggtggaactg tacgccatag aagcgttttt cttcgttggc catgatggca
     2281 aacgggcagc tttcggtgct ggctacagtg acgaagtcgg acgggatagc ggtaacttta
     2341 tcgccgtggc tcatccagac gtccagcagc ggtttaccat ctgcggtcag cgcatcttcg
     2401 ataccgcgaa ccagtgcgct gtcgtttacg acttcaacct gcgcgtagcc aaattcacgt
     2461 tcattagaag cctcaacgtg accgcccaac tgcatcgcca tggtctgcat accatagcaa
     2521 acgccgaata ccggtacgcc tgcttcaaag acatactgcg gcgcacgcgg gctgttttct
     2581 tcagtagtac tttccgggcc gccggaaaga ataatgccgc ttggattgaa gtcacgaatt
     2641 tgtgcttctg tcacatccca cgcccacagt tcgcagtaaa cccccagttc acgcacgcgg
     2701 cgcgcaacca gttgagtata ctgggaaccg aagtccagaa tgaggatgcg atgcttatga
     2761 atgttttccg tcattgacgc ttattccgag gcaagtgaaa cagataatat aaatcgcccg
     2821 acatgaagtc gggcgaagag aatcaggagc ccagacggta gttcggggac tctttagtaa
     2881 tggtcacgtc gtgaacgtgg ctttcctgaa tgcccgcacc gctgatacgt acaaactccg
     2941 ctttagtacg cagttcgtcg atagtaccac agccggtcag gcccatacag gagcgcaggc
     3001 cgcccatctg ctggtgaatg atctctttca ggcgaccttt ataggctacg cggccttcga
     3061 taccttctgg caccagtttg tcggcagcgt tgtcgctctg gaagtaacgg tcagaagaac
     3121 ctttggacat cgcgcccagg gaacccatac cacggtaaga tttgtaagaa cggccctggt
     3181 agagttcgat ttcacccgga gattcttcag tacctgccag catggaacct accatcaccg
     3241 cgcttgcgcc agcggcgata gctttagcga tatcgcctga gaaacggata ccgccgtcag
     3301 cgataaccgg aatgccggta ccttccaggg cttctactgc atcagcaacg gcggtgatct
     3361 gcggaacacc gacaccagtc acgatacgcg tcgtacagat agaaccaggg ccgataccca
     3421 ctttaacggc actgcaaccg gcttctgcca gcgcgcgggc acctgcagca gttgccacgt
     3481 tgccgccgat gatttgcagg tcaggatatt tagcgcgagt ttcacggata cgctgcagaa
     3541 caccttcaga gtgaccgtga gaggagtcga tcagcagaac gtcaacgcct gcggcaacca
     3601 gcgcatcaac acgctcttcg ttaccagcgc ccgcgccaac cgctgcacct acacgcagac
     3661 ggccttgttc gtctttacac gcgttcggtt tacgttccgc tttctggaag tccttcacag
     3721 tgatcatgcc gatcaggtgg aattcatcat caaccaccag cgctttttca acgcgttttt
     3781 cgtgcatttt tgccagcacc acttcacggg cttcgccttc acgcacggtt accagacgct
     3841 ctttcggcgt catgtaaacg ctaaccggct ggctcaagtc ggtcacaaaa cgcacgtcac
     3901 gaccggtgat aatgccgacc agttcgtttt cttcggtaac aaccgggtag ccagcaaagc
     3961 cgttacgctc ggtcagttct ttcacttcac gcagcgtcgt ggttggcagt acggtctgcg
     4021 gatcggttac cacgccagat tcgtgttttt tcacacggcg aacttcttct gcctggcgtt
     4081 caatggacat gtttttgtgg ataaagccga taccgccttc ctgagccaga gcaatagcca
     4141 ggcgcgcttc cgttacggta tccattgctg cggaaagcat agggatattc agacgaatag
     4201 ttttcgtcag ctgagtgctg aggtcagcag tattcggcag aacggtagaa tgagcaggaa
     4261 cgaggagaac gtcgtcaaac gtcagagctt ctttagcgat acgtagcatg ggcaatatct
     4321 cgaccagagt ggttaataaa tattgccgcg gcattataca gagcgtaacc gattgcatct
     4381 accccttttt gcaaaaaatg cttgctatcc ccggagggcg ggttactatc gactgaataa
     4441 cctgctgatt tagaatttga tctcgctcac atgttacctt ctcaatcccc tgcaattttt
     4501 accgttagcc gcctgaatca aacggttcgt ctgctgcttg agcatgagat ggggcaggtg
     4561 tggatcagcg gcgaaatctc taattttaca caaccagcct ccggtcactg gtactttacg
     4621 cttaaagacg ataccgcgca ggtgcgctgt gcgatgttcc gaaacagcaa ccgccgggtc
     4681 accttccgcc cacagcacgg gcaacaggtt ttagttcgcg ccaatattac actctacgag
     4741 ccgcgcggcg attatcaaat tatcgtcgaa agtatgcagc cggccggtga aggattgctg
     4801 caacagaagt acgaacagct taaagcgaag ttgcaggctg aaggtttgtt cgatctgcaa
     4861 tacaaaaaat cactcccctc ccctgcgcat tgcgttggtg tgatcacctc aaaaaccggt
     4921 gctgcgctac atgatatttt gcatgtgtta aaacgtcgcg atccttctct gccggtgatc
     4981 atctacccca ccgccgtaca gggggatgat gcgccggggc aaattgttcg cgccattgaa
     5041 ctggcgaatc agcgcaacga gtgtgacgta ttaatcgtcg ggcgcggcgg cggttcgctg
     5101 gaagatttat ggagttttaa cgacgaacgc gtagcgcggg cgatttttgc cagccgcatt
     5161 ccggtagtga gtgccgtcgg gcatgaaacc gatgtgacaa ttgctgactt tgttgctgat
     5221 ctacgtgcgc caacgccgtc agccgccgct gaagtagtga gccgaaatca gcaagagtta
     5281 ctgcgtcagg tgcaatcggc ccgtcaacgg ctggagatgg cgatggatta ttatctcgcc
     5341 aaccgcacgc gtcgttttac gcagatccat catcgcttac agcagcagca tccacagctc
     5401 cggctggcac gccagcaaac catgcttgag cgcctgcaaa agcggatgag ctttgcgctg
     5461 gaaaatcagc ttaagcgtgc cgggcaacag cagcagcgat taacacagag gctaaaccag
     5521 caaaatccgc agccgcgtat tcaccgcgca cagacgcgaa ttcaacaact ggaatatcgc
     5581 ctggcagaaa cgttgcgcgc gcaactgagc gcaacgcgag aacggtttgg taatgcagta
     5641 acgcacctgg aagcagttag cccgctttcg acgctggcgc gtggttatag cgtcaccagc
     5701 gccgcagatg gcgcggtgtt aaagcaggtc aaacaggtaa aagcgggcga tacgctgacc
     5761 acgcgtttga atgatggcgt ggtggtaagc gtagtgagcg acgtggcgaa aacccgcaaa
     5821 ccacgcaaaa aaaacagcta attcgtagct cccaggccac gccagatgcg ccatgctggt
     5881 tactggaagc aggtcaccag acccggttta tttattagcg tgatactaca atcctgtgct
     5941 ccagtacctt cgtccatccc acactgttga gaggacaaat aaggaaaact gggtgaagac
     6001 ggccatccca gtgaattaaa aacagtatgc aacaactcta attcattgta tgtcggtgca
     6061 ttttctatgc tgccacactg ccttgcgata tcccatttac tggcatcaat gatatgtgca
     6121 gaagcccagt tttcgtagtt aataatcggt gaggatacgg gattagcccc ggacggcaat
     6181 tctgtcgtta acaacggtcg atgtagcgat ttaccattca ccactaccgt gtctgccata
     6241 tgtccccaaa agtgcgccga ggaaacattt gggctggttg gtaccgtaaa aatagcgtcc
     6301 agcgtgtctg tcgcggaagc gtaattagcc agagtaaccg tcagtggcgt tttataacca
     6361 ggggtattat cctggcggag cgtaaaactt gccgtcccat ccgcgcctgt catgccacta
     6421 atggtattac cgctttcact cagcgtaata gccgaaccag ataacggctg aacgatgaga
     6481 tcgtccatcg ccactacatt gccatacaac gttgccccag cgttacgcgg cgatgcctca
     6541 ccacgtttca aagtaaattc aacattcggc accgtattcc cggcgctgtc tttcaccgtc
     6601 acggtaacgg acatggcttc gccttttttc actttcgccg cctggctgtc agcatcaaac
     6661 gctgttgagg tcagtgtgac gctggaaacg gttgaacgcg cgttagccag gcaaggctga
     6721 aatgattttg ttgaacccgt atacagcgtg ccatttctca aatcaaccga ctggtcttta
     6781 ccatcggcgg atttatcctc tgcccaccag gtgtaatacg caggccagcc agtttcagtg
     6841 acaaacgtgt tattcggata acgcgcagac aacgaactta aatcattgaa tagtggctgc
     6901 cttgctactt cacaagggct cacactcgca tctgatttct gtgttggggt aaagtacggc
     6961 cagtcttcat tattgttatt gatcttgccg acgccagaag tcgatgtcag ttcactccac
     7021 aatttcgggc gttcaaatac cgcaccattc actgccgtca aggttgctgg catatgcccc
     7081 cagtagttag ccttcgcaga atccgggctg gtcactaccg tgaatatcag gtcctgatta
     7141 gccgtaacct gcggatttcg cgccaatgaa gctgtaaacg gcgttaccag accgacggtg
     7201 ttattttggg taacgttgaa cgttatcgtg ccatcactgc cggtgaagcc gctccatgcc
     7261 gacgtattct ggttgaagga gatacgcgcc gatgttgggg tgacttcatc taaaatgaga
     7321 tcatctacgg tattggagga tacagagatc cctgaacggc ttttcgcctg cacgcgcttg
     7381 agcgtaacac cttcgccgcc aacaggatta ccattggcat ctttgatcgt caccacgatg
     7441 ggaagcatct cacctttttt cacagatgac gtgggggtac caccattgct ggcagttcgg
     7501 ccctcatcat aggcagtcga tgacagcgta aggcttgctg gtgccgaatg tggctgcgtc
     7561 agacagagca ttaaagcgta tccatttata gtcgtcgtcg attgtcccgt tttcaaatta
     7621 atgacgccgt aataccacgt caacgcgtca gtggtgctca tttcataagt ccaccaattt
     7681 ccacttccgg cagttatagg caaccccaaa tctgtcagta ttgtcccgcc tggatgatca
     7741 ttatatagcc cctgtaactc attgagacgc ggctgatact caaccgggca atccggcgta
     7801 actttattgg tataaaaaag gtatgaccac gactcattat tattacttac cgaggacccc
     7861 aaggtcgctt ctgctgccag tagcggtcgt ttaaaggtca cccccgccct actggtaaac
     7921 gtgtcgggca tatgacccca gtattttgct ttcggtgagt tcggactggt tatcacagtg
     7981 aaaataacat ccatcgtggc actttttgtg gtgtcgcgca tcaatgttgc agtgagcgga
     8041 gttgccagcc cataagtctc gttttgcgtc acatcaaaag tcgctttacc atcacttcct
     8101 gtcgtcagaa gtgcctgtgc cccattatta tagagtgaca tgtttactcc ggcaggggtg
     8161 accggcacga tagtaagatc atcatacgta gaatctagcc tgttttttgc acgatccaac
     8221 gcagtcccgc gtatgagatt aaagtttgca cctggcaaca gatttcctgc gctatcccgc
     8281 acaatcaccg tcatccggat cgccgtacct ttcgccgccg ttgcactagg gcgaccatcg
     8341 ttgctggccg ttttctccgc atccagtgcc gtggaggtca tctctataga atctgccact
     8401 gcatgagggt ttaccagaca ggacacaaat gccacggtgt ttgataatgc cctggagctt
     8461 gatccattcg ataaattaat agactgatcg taccagacgt tcgcgtaggc aattttatcg
     8521 taagcccacc aggtgttagt cagcggaagg cctaaatcgg tccctatcgc accattagga
     8581 tgttcactgt ataactcttg aaattcgctt aataacggtt gataaggttc gccgcagtct
     8641 gatttgctga catcattttt tgctgccgta ttcaaagaaa gccatgattc attattagcc
     8701 agggttgact gccccgctgg atgctcggca gtaagcaatg gccgcttaaa ggcaattccc
     8761 gaccgggtag taaacgtttc aggcatatgc ccccagtaac ttgccagtgg cgtatccggg
     8821 ctggttatga cggtaaacat gaccggtaat ttgttggatt ctatcgtcgg ctgatacaac
     8881 tgggcgtaca tattggttac caggcccata gaatcatctt gttttaccgt aaaggaggta
     8941 gtcccgtccg cgccagtcac cccataaagc aggcttgaag aactattcat cctcgcagaa
     9001 gcgcctgccg cgttaattac cgtaatcgac ttatttatca tctcggcata ttgacggttc
     9061 ttcgagcctt cacgtctgag aataaagggg acattgggca cgctatttcc ctgcgcattt
     9121 ttagtcacca cgcgaatcac cgcgtcttcg ccttttttca ctttcaacgc actcaaggtg
     9181 tcgtcaactt tgacaaattg cgccgtatca ttcgcctcaa caatgatctg actggcaacg
     9241 gcgacaggcg tggtccggca gctcagtaat gcaccaccag atgagggcat ctgcgtttca
     9301 tctcctgtct tcagcgaaac gacatcatac gtaaaggttt gagtgaacgt atctgcaacg
     9361 gtattcgaga tatagaacct cgacactggc cagccaatag catcatgtaa tgcgctactc
     9421 ccctggtgcg cataaatggc cctaagttca ttgtcagttg gaacatactc cgcgccacaa
     9481 ccatttatcg aggtatcgtt agcctggccc gacgggaacg tagaccaggc ctccccctct
     9541 tccaccaggg taccgatact ggtggtagca ctattctcgt ccctcaacaa aggccttcga
     9601 taaataagac cgtcatcgcc tgtcgccgtt tcagccatat gcccccagaa tttggctttt
     9661 ggcgaatccg ggctggtaag caccgtaaag acaacgggaa gcgtcgattt agtgctgctg
     9721 gaggaatcca acgctgccgt gagttctgtt tttagcccgg tagtctggtc ttgcttcaat
     9781 gtgaaagtgg tggtgccatc agcccccgtc acgccataaa taaccagggt cgttgaaaga
     9841 aaatcatttt gagtatttcc ccaggcatcc gttaccgcca gcgaagcaat cgacgtcgta
     9901 attgacacat tggccctgtt cacgctggtg tttcgtttga gggtaaatgc ggtatttccc
     9961 accgggttac cctgcgaatc tttagtggtc acgcgaacca cgatttcttc cccttttttc
    10021 gctttgacaa cattattcgt actgtcaaat ttcgcggggt ccgcagcctc aagaacgatt
    10081 tggcttgcct taacgatcgg cgtcgtctgg caggtaagca gttcttttgt ggcagatgga
    10141 attatcgaac tggtgcctga actgagattg acggccttga agtcacgctg ttccaggcta
    10201 ctactacctg tcgtgctgga aaggtaactt gaggcgacgg gccagccctg aaccgttttc
    10261 atcgcattgc cgcggttggc ttcatacaat gactccagcc cggcctggga gggaacatat
    10321 tcactaccac aaccattaga ggatgtgctt atagcctggg tttgggtaaa ttgcgcccag
    10381 tcttcattct cttctgcaat cgctgttcgc cccgacgtac tggataactc tttcaacaat
    10441 aatgggcgtt taaagaccgc tccatctgca gccgtcactg tttcaggcat atgcccccac
    10501 attttcgcct tatcgctgtc cggactggtt acaacggtaa aaatggtatc tagggtcgct
    10561 tttttcgttg tatctgaata gagcgtagcg gtcagcgacg ttttcgtgcc atgggtatcc
    10621 gggcgggtaa tagtgagaat tttagttccg tcgctgccgg ttatggcgct gtatgctgca
    10681 gcggtattat taagcgaagt ttcgtccgcc aggccaccat tgaccacaac cagcgcatca
    10741 ccgctaccag caacgtgttt ttcctctgag cgggtataac catcgccacg tttcagggta
    10801 aagggtatat cacccaatgg attgccctgc gcatctttta ccgtcacctt tacctgtagc
    10861 gtttcgcctt ttttcagctt cgccgcattt gcagccgcgt tccactgcgc cggatccacc
    10921 acttccagcg taatgctact ggcaggattg ttagccgtcg tcaggcaact gacataaacc
    10981 gccgtgctac cgccaacggt ccgcgcccca tcgttgaggg ctatggtgta ataatgtggt
    11041 acctgatctg cgggcgaact gctccagtag ggctgacgct gcgttggcca gccgtgtact
    11101 gtctgtacac cgtcaccgtt attggcatca tagagcgcgc taagctgaga acggcgtggg
    11161 agcatattgg cgccacagcc acctttactg gtgctggagg cgttggcctg gctgactcgc
    11221 gaccagactt cgttattttc cgttaatgtg ccgtccggac tgaacacttc cgcagccagt
    11281 cggggtcggg caaaggttaa agcatcgacg gtaatcgtct catccatatg cccccacatc
    11341 tgcgcgccaa ccgcatccgg actggtgagc gtagtgaaaa tcacattgta attaacagta
    11401 ttgggaatat aggagttcac aggcacaata tttagcggcg tcgataagcc cacaccttgc
    11461 gattgcttaa tttccacagt cgcaaaaccc tgcgcatccg tagtaccggc atagactaaa
    11521 gacggttgcg atgttccgta gagtgtgcca ttcatctcga ttgcgccgcg ggttggatca
    11581 tcaaaacctg tagtctgccc ctgacgattt ttccccatat ctttagtgat ggtgaacgcg
    11641 gtatacggca cgggtgcatt attcaaggca ttaaaggtgc gtaccgtcat ggtgatcgtg
    11701 tcacccactt tggcctgcgc tctgctgcct gcggaaacat cacgatcggt ggtcgcggca
    11761 atcttggcga ccatctcatt gcccgaacaa gagagatagt tttgcttaaa gccggaatag
    11821 gtgtcgatgt tgcccgtggc caggtttacc gacgcgtgcg acgtctcttc caccgcacta
    11881 agatacgctt tttgcaacgt tggccagcca tattcggtgc cgatggcatt ggctggatga
    11941 gcggcaaaca aactgtgcaa tgaactttgg ctcggaatat gacctaaacc acactcagcc
    12001 tgcatactgc tattctgatc aaacagcgcc cagtcttcgt tattttcccg caccgatccc
    12061 agttcgttgt cggtttcatc cgccagcctt gggcgtttaa agatattgtt cgcttcaata
    12121 atgcccaaca tatggcccca catccgcgct ttgtcggtat cggggctggt gatcaccgta
    12181 aaaataacgt ctttctcatc cgaggcagta aaatcactgc gcattctcgc ggtgatgtga
    12241 gttttcaccc ccacgccgcc tggctgggtc agcgtcagag tggcctctcc gttagcatca
    12301 gtgattcctt cataggtatg ggcgtcaact ttgcgaacgt tactgccgcc atcaatttgt
    12361 accggatgcg tctcccacgc ggcgtcagtt tgattcttac ggttaatgcc gtcatccaga
    12421 tgcagcgaaa agtagtagta agccagcggc tgatcgttgt tcttgctatc ggtaatcgtc
    12481 agacgtaaag aagcctcttc ccccaccttt actttcatcg cttttatcgt cgaatcatag
    12541 ctgcccggcg tcagtaccag cttcggttca acatcgggcg cttctttatc gacacagctc
    12601 acaataaacg tcgtatcgtc cggtttttgc gcttcgctgc ggttcgaaac atccgccgca
    12661 tggtgctggc cgctcatccc cgccagtgat gaccagtaga aattaccctg catcggccaa
    12721 ccagccacat cctgcactga cgttggcacc accgtcgcca gcgcaccgaa ctgacgcatt
    12781 ccgggcaaaa tgtcgcaatg tttatccgcg ccgctccagg taaaggtgga ccaggtttca
    12841 ttgtgatcgt taaccgtacc gccttcatta tcgacttccg ccgccagttt tggtcgacta
    12901 aaagtgtaat cacgggcctt cagcgtatcg ggcatatgac cccacattgt agcctgcgga
    12961 acatccgggc tggtgaggac ggtaaagatc actgccgttt cactggcctg agcaataccc
    13021 gcgaggctcg cgaccaacgg cgtttttaca ccagggccgt tggcctgggt gaccgtaacc
    13081 gtggcaacac cattcgcatc ggtagtacca cgatattccg tcgcggtggt ggttaattcg
    13141 gtcgtgccca gttttacagg cgcagtattg ttcaccacac cctggcggtt ttcagcatct
    13201 ttgcgtttga taacaaaagg aatattcccc acaacattac cgacgcaatc tttggtggtg
    13261 accgttaacg tgatactgcc cccaaccgtc attttcgccg cacctgagga ttcgtcatag
    13321 cccgcagcgc tgacggaaat aaggtcaccc tgcgcgggcg caatcttcag cgtcactttc
    13381 tgatcggcga gcgtcagaac actttcatag ggatagcccg tcgaggtgag taaatataac
    13441 agcggcatgg tctgctgggg atcaggctgc gcccaggcga ccagcgaacg cccggcaagt
    13501 ccctgtgcac aaaacgagcg atctgcgact ggctgtgggt ccagtaaggt gcccggcgtc
    13561 gcggcatccg cccaaactaa agatattgcc ggtggcgttt ggttttccca acgtggggtg
    13621 gcaatgatat cgccttctct gtcttgcgga ttggtgacga gcaatttaga gacgctaaca
    13681 tccgcgctga attcactcgg tttagcggta aacgctacat catgcgtctt tgcaggatcg
    13741 agaaagacac tgccctggta gacgggtatg ctggcgctgt cggcagtagg cacggtaccg
    13801 ttaaattcgc cgaccgtatc tgaagagtct tgccatgcag attctgccca tgaccaccca
    13861 ggcagcattg cgcttatcag caatgcgcat aacacaagga ttttattcag ggtcagagac
    13921 atgacagaaa ctccaccggt tgttaaacgg acgcttgtcg catcaacata ttttgcttaa
    13981 tccgggatta agcaaaatat gcggaaacag cattcccttg cgggaatgct gctgagtacg
    14041 cgttatttgt gtttgtaaat aatgtgaagt ttgtaaccct gtaccccgtc gccgttggtc
    14101 gggatggaaa gtgcctcttt tccatcgcgc gcagggccgt taaggttggt tctggcttcg
    14161 tcgttggtcg cagggatgac caaattgtta ttatcgaagc ttgagttaac gatgccaccc
    14221 gaagtgccaa gctgttggct gctgccagaa agtacccaac ggaagtcata attggcggtg
    14281 acgtcagcat cggtacccgc atcaaacttg ccatttttgt tctcatccga gtacagtttg
    14341 gccacgtagg ttttgccaag ttcgattggc gtgctaccgt tgataaggtt gatactggta
    14401 ccatcaacaa agatggcgac tttcaggttc tggttaacca ccggccccgg tgcaacgtta
    14461 tcgctgtcgc ttccgccgcc gctggcagta gaaacgtcat aaagatgcag cgcagtcccg
    14521 acgtttggcg taccggtctg cgtggttggc gtaatttcaa taccgatata gcgaccacgg
    14581 tcggcatcgg taagagtgta actggtcacc ttcgttgcca gctcttttgg atcccccccc
    14641 tgcgcatcct tgtagctcat ccagcggata gtatcgatag ttttcgcgtt atcgcccaat
    14701 cctccaagat ccacgtcgcc ttcggtatca ccaatttccc aacttacggt taatttatca
    14761 cccgagtaaa gacgcttgtc cgtttcactg ccttgcgggc gagcgccttt tagatcagaa
    14821 gtcaacgtta cacggtcggc atctgtcgta gcacttcccc cttccgaata gagccacgga
    14881 atggtcccct ggaaaatatt ggtttcgccg ctgtcgaccc aggcactgtc ttcagccagc
    14941 gccacaggtg ccacacaata acctgccagt gccagcgcta atgcgatccg ggtcagaccg
    15001 cgtcttttat cctgcttcat tgatgctctc cttaatgaac cttcagcgac tggccgactg
    15061 gccttacgcc aatcttattt tttggtatac agcacgcgta aaccgtatcc ctgcaagcca
    15121 tctcccgctt gtgcgccata ggtttgcgcc gcttcacggt tagtggccgg aatcacaatg
    15181 tcctcattgg aagtgttcgc ctgtgcaccc acttcaccat tgatgctctt gccggtaaat
    15241 tcccacttgt agtcgtactg cgccatctct tcgtcagtca gtttttcgac ctgctggaag
    15301 acaccgtctt tattgttgtc gcgccataaa acaaatcgat aaatgttgtt aagcggcaac
    15361 ggttctttat ctttacctat caggttgctg ggtttagccg cccccactcg atagataaaa
    15421 gcatttagcc caccaatttc tgccccgagg aaagtgacat ccacatagtt actgtcatcg
    15481 tagggcgcgg cttttgcttt ccagcgatag gtgccgggta aggtactgga gagggtgaca
    15541 gtgccctttt ctttatcttg tttagcgata aaaacgcagg ggttgtcggt cgagcaaggc
    15601 ttgttggtta acgtcactcg tttgtcctgc gcgtcgtaaa gctgcggcgt gaggttatct
    15661 tccggcagcg caccgccatt gcggtcggtg ttatcgccaa agctgtcggt tacatcgaga
    15721 tccatcacca caccatcaac gccatctgcc tgagcgctgg cttcgtggct gtggttgttc
    15781 tcttctggca cgttcgcgat gttattaaca atcaactcta ttttacggtc ctgctgaacg
    15841 gtgatttcca ccacgccaga gttctgtcgg ttgccctttt catcttccag cgccagacgt
    15901 actcgccagg tattgagttt ttcttgttcg gcttgatcgg aactgtactg ccaggcaggc
    15961 agcaccaggt tccagtggtt attgttaccc tctgtcagag tattctcttt gataaccggc
    16021 atcaccagcg tgcgggcagc attgtttttc gcctgccagg aggcagcaac gattttgtaa
    16081 tgatgattaa tgagtgcgtt aatggtccac ttacattttt ccagaccgac ggcttcttcg
    16141 ggcgtatctt taatcacgca ctcgggatgt tcattcgtga cgtcggcttt cagccacaaa
    16201 gtgacataga ggggatcttt ctctttgtat tccagaacaa taaagttgtt acgttcaaca
    16261 aagtcatgac ggctacccat gagcgagtga gccgcgctca ctttgtcagg atcaagctgg
    16321 tctttgagcg ggacgccaaa ctggtagttc agcgttgcgt taacagaaag atcggtatta
    16381 tcgccagtgc cagctttgaa atctgtaccg acaacaatga gcggaacggg ctggtatttc
    16441 acacccagcg tgacagcgaa gggatctttc tccagactat cggtaccaaa aagcgcgact
    16501 tcattcccat aatattgttc gaagacaatt tttccgccaa gctgcgggta agcgggtagc
    16561 caggcttcag cgcgaatatc ccagccgcgc gcagggcgtt cttcataaaa gtcgaaatct
    16621 tcagagtctt tccagtcaga aagtgggtga taatagttgc ctgagaattt taaataatcc
    16681 gtccaggctt cggcgcctaa acctaaacgg cgatgtccac gggtaaagtc ataatcataa
    16741 aatatatttc cacccaatag atatttatca aaattatacc ttaccccaag gccaatattc
    16801 ccgatcgtac gatcttcttt tcgttgcgca gaaaattggc tgaaataaac agtggtttga
    16861 ttatcgtacc agggaacaaa ataatcgatg gaactgccat ccagatcgcc gccctgacca
    16921 atggagaggt tcgaacggac cttaccgtag ggagagagtg tgtcttcaat ataagattgt
    16981 gcggttgagg tgatttgact ttgcagatag cttctggcct gagattccag cgcctcaggg
    17041 gtcaagtttt caaacccttg cgtggcggag ttgatgacgt aatcggctcc gcgctctttc
    17101 agcgatttac ccttgttggt ttgttcatcc tgttgtgctg cctggctacc taaatcaggt
    17161 aacgattcgt tttgcttaac atttatctct ggcgccgcga aagcagcacc agagataaat
    17221 gttagaataa tacagcgttt ccatcgcagc atgcactttc cctgtgacaa tgatacagtc
    17281 aaaaataagc tttaatattg tatccctgat tattcagaaa ggcatgtcat aaagagtaaa
    17341 tatattctta attatttaaa ttagtgattt cact
//
LOCUS       DADSYM010000106        16831 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2415
            SAMN08039579-rid11723173.denovo.106, whole genome shotgun sequence.
ACCESSION   DADSYM010000106 DADSYM010000000
VERSION     DADSYM010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039579
            Sequence Read Archive: SRR6362533
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16831)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16831)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980162.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 03:39:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,484
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 4,347
            CDSs (with protein)               :: 4,347
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 43 of 85
            Pseudo Genes (incomplete)         :: 37 of 85
            Pseudo Genes (internal stop)      :: 19 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16831
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039579-rid11723173.denovo.106"
                     /strain="LGE2415"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8302 N 95.9628 W"
                     /collection_date="2015-08-24"
     gene            77..1309
                     /gene="rbsK"
                     /locus_tag="J1Y83_003052"
     CDS             77..1309
                     /gene="rbsK"
                     /locus_tag="J1Y83_003052"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000285497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HBA7631180.1"
                     /translation="MYRDTRREHIVRLLRNKDECSVSDLANYFSVTKETIRTDLAWLQ
                     KRGVVSRHHGGVSLKKHLMQNALSQQEYVDMSLLIKQQQQGIGYLTSLDDKGRRMVGK
                     VCVLGSFNVDIVAKVYRFPNEGETLVARETTMGPGGKGANQALAAHRAGAQIHFACKI
                     GRDQFNHFARNHIESIGIDSFTFYESELSTTGCAVIYVNDEGENMIAMSPGANHELSD
                     NDIIQLSHFIAESDVFIVQMENNLAATQLALKCAQKMQVTTILNPAPWSSDVATLLPF
                     VDIVTPNETEASAMSGMVIDNISDAVKAAKHIYNAGQCSVIITMGKRGALIYDGQQCA
                     HIPAFSAVAVDTTGAGDAFNGALAAALAKGESLVKAAWYASAFASLAVEKEGAANMPD
                     ISLVDARIQQQNVAIQTF"
     gene            1335..2258
                     /locus_tag="J1Y83_003053"
     CDS             1335..2258
                     /locus_tag="J1Y83_003053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000848329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HBA7631181.1"
                     /translation="MKTIEGIIPVMLTPFTADNKIDYLSLKRLIDWYIENGADALFAV
                     CQSSEMQYLTLEERVELASFVVEYSQQRVPVIASGHISDDLEQQIQELNAMAATGIDA
                     LVLVTNHLDPHNEGTTVFLRTLDALLMSLPENMALGLYECPAPYRRLLTDEELLYCAQ
                     SGRFQILKDVSCDLPTVTRRVQLTQHTPMTIVNANAAIAWEAMLAGSKGFCGVFTNFH
                     PDLYHWLWLEGKQHPEEAKQLAWFLSLSAVSENIGYPKNAKIFHQQRGTFDCVHCRVT
                     ADDVLKKYWGLEMVLKQIADAADFQRQQLKY"
     gene            2313..3265
                     /locus_tag="J1Y83_003054"
                     /pseudo
     CDS             2313..3265
                     /locus_tag="J1Y83_003054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000831650.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
     gene            3314..3751
                     /locus_tag="J1Y83_003055"
     CDS             3314..3751
                     /locus_tag="J1Y83_003055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001220300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter TctB
                     family protein"
                     /protein_id="HBA7631182.1"
                     /translation="MRIIPVTPLFLLIFAMFIFGWSITQYGGISQYGAGFMPCIISGL
                     LLFLAAIDVAIDIKHRHRNKVSLTLDEVKALLLVITVVLLFVLLLDILGFVICSFLLL
                     FILMKIRGVRLLHSVIVSISASLIIWFIFARVLLVAFPEGILF"
     gene            3761..5248
                     /locus_tag="J1Y83_003056"
     CDS             3761..5248
                     /locus_tag="J1Y83_003056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000350780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter permease"
                     /protein_id="HBA7631183.1"
                     /translation="MDILIQALTYINLNAILAIIAATAFGLFVGAIPGLTATMAVALM
                     VPFTFFLDPIPALALMIAVGASSIYAGDIPGALLRIPGTPASAAYVADSNELVKQGKV
                     NRVLGLGLVSSVIGGVIGSLILMLAAPVLAKFALKFSSYEYTWLSLLGLTCATLVVGN
                     QPLKGMVTLCLGLLLACIGYDQISGVPRFTFGQISLLQGVNFIPAMIGLFAISGAIEY
                     YRDKISQVSGAVPQTQTGINLFKGMGQVIWSRKLNLGRSSIIGTLIGALPGAGADIAA
                     WISYAISKKFSRQQELYGHGSEEAIVDSSSSNNASLAGSWIPSLVFGIPGDSAAAIII
                     GVLYMKDMNPGPTLFLFQADKLYAVFILFLIANIALLPLATIAVSFIKRIIWIDKAIL
                     YPIILIFSIVGAFAIDNSGASVVVMLVMGVLGYWLQRKEYPVSPIILGMILGPMLEKN
                     LLSSMIKSNGEWLAFVERPVSMALAACFFLVVALQGRNIYRSFSR"
     gene            5432..6196
                     /locus_tag="J1Y83_003057"
     CDS             5432..6196
                     /locus_tag="J1Y83_003057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HBA7631184.1"
                     /translation="MQALLEHFITQSTVYSLMAVVLVAFLESLALVGLILPGTVLMAG
                     LGALIGSGELSFWHAWLAGIVGCLLGDWISFWLGWRFKKPLHRWSFLKKNKALLDKTE
                     HALHQHSMFTILVGRFVGPTRPLVPMVAGMLDLPVAKFITPNIIGCLLWPPFYFLPGI
                     LAGAAIDIPAGMQSGEFKWLLLATAVFLWVGGWLCWRLWRSGKATDRLSHYLSRGRLL
                     WLTPLISAIGVVALVVLIRHPLMPVYIDILRKVVGV"
     gene            complement(6310..7008)
                     /gene="thiQ"
                     /locus_tag="J1Y83_003058"
     CDS             complement(6310..7008)
                     /gene="thiQ"
                     /locus_tag="J1Y83_003058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter ATP-binding protein
                     ThiQ"
                     /protein_id="HBA7631185.1"
                     /translation="MLKLTDITWLYHHLPMRFSLTVERGEQLAILGPSGAGKSTLLNL
                     IAGFLTPASGLLTIDGVDHTTTPPSRRPVSMLFQENNLFSHLTVAQNIGLGLNPGLKL
                     NAAQQKKMHAIAHQMGIDNLMARLPGELSGGQRQRVALARCLVREQPILLLDEPFSAL
                     DPALRQEMLTLVSTSCQQQKMTLLMVSHSVEDAARIATRSVVVADGRIAWQGKTDELL
                     SGKASASALLGIKG"
     gene            complement(6992..8602)
                     /gene="thiP"
                     /locus_tag="J1Y83_003059"
     CDS             complement(6992..8602)
                     /gene="thiP"
                     /locus_tag="J1Y83_003059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine/thiamine pyrophosphate ABC transporter
                     permease ThiP"
                     /protein_id="HBA7631186.1"
                     /translation="MATRRQPLIPGWLIPGVSAATLVVAVALAAFLALWWNAPQGDWV
                     AVWQDSYLWHVVRFSFWQAFLSAQLSVVPAIFLARALYRRRFPGRQMLLRLCAMTLIL
                     PVLVAVFGILSVYGRQGWLASLWQSLGLEWTFSPYGLQGILLAHVFFNLPMASRLLLQ
                     ALENIPGEQRQLAAQLGMRGCHFFRFVEWPWLRRQIPPVAALIFMLCFASFATVLSLG
                     GGPQATTIELAIYQALSYDYDPARAAMLALIQMVCCLGLVLLSQRLSKAIAPGTTLLQ
                     GWRDPDDRLHSRICDTALIVLALLLLLPPLLAVIVDGVNRQLPEVLAQPVLWQALWTS
                     LRIALAAGVLCVVLTMMLLWSSRELRARQKMLAGQALEMSGMLILAMPGIVLATGFFL
                     LLNNTIGLPQSADGIVIFTNALMAIPYALKVLENPMRDITARYSMLCQSLGIEGWSRL
                     KVVELRALKRPLAQALAFACVLSIGDFGVVALFGNDDFRTLPFYLYQQIGSYRSQDGA
                     VTALILLLLCFLLFTVIEKIPGRNVKTD"
     gene            complement(8578..9561)
                     /gene="thiB"
                     /locus_tag="J1Y83_003060"
     CDS             complement(8578..9561)
                     /gene="thiB"
                     /locus_tag="J1Y83_003060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter substrate binding
                     subunit"
                     /protein_id="HBA7631187.1"
                     /translation="MLKKCLPLLLLCTAPVFAKPVLTVYTYDSFAADWGPGPVVKKAF
                     EADCNCELKLVALEDGVSLLNRLRMEGKNSKADVVLGLDNNLLDAASKTGLFAKSGVA
                     AEAVNVPGGWNNDTFVPFDYGYFAFVYDKNKLKNPPQSLKELVESDQNWRVIYEDPRT
                     STPGLGLLLWMQKVYGDNAPQAWQKLAKKTVTVTKGWSEAYGLFLKGESDLVLSYTTS
                     PAYHILEEKKDNYAATNFSEGHYLQVEVAARTAASKQPELAQKFLQFMVSPAFQNAIP
                     TGNWMYPVANVTLPAGFEQLTKPATTLEFTPAEVAAQRQAWISEWQRAVSR"
     regulatory      complement(9592..9691)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(9725..11380)
                     /locus_tag="J1Y83_003061"
     CDS             complement(9725..11380)
                     /locus_tag="J1Y83_003061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator SgrR"
                     /protein_id="HBA7631188.1"
                     /translation="MPSARLQQQFIRLWQCCEGKSQETTLNELAALLSCSRRHMRTLL
                     NTMQDRGWLTWEAEVGRGKRSRLTFLYTGLALQQQRAEDLLEQDRIDQLVQLVGDKAT
                     VRQMLVSHLGRSFRQGRHILRVLYYRPLRNLLPGSALRRSETHIARQIFSSLTRINEE
                     NGELEADIAHHWQQISPLHWRFFLRPGVHFHHGRELEMDDVISSLKRINTLPLYSHIA
                     DIVSPTPWTLDIHLTQPDRWLPLLLGQVPAMILPREWETLSNFASHPIGTGPYAVIRN
                     STNQLKIQAFDDFFGYRALIDEVNVWVLPEIADEPAGGLMLKGPQGEEKEIESRLEEG
                     CYYLLFDSRTHRGANQQVRDWVSYVLSPTNLVYFAEEQYQQLWFPAYGLLPRWHHARP
                     THCEKPAGLENLTLTFYQDHIEHRVIAGIMQQILASHQVTLKIKEISYDQWHEGEIES
                     DIWLNSANFTLPLDFSLFAHLCEVPLLQHCIPIDWQADAARWRNGEMNLANWCQQLVA
                     SKAMVPLIHHWLIIQGQRSMRGLRMNTLGWFDFKSAWFAPPDP"
     gene            11469..11600
                     /gene="sgrT"
                     /locus_tag="J1Y83_003062"
     CDS             11469..11600
                     /gene="sgrT"
                     /locus_tag="J1Y83_003062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001248776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake inhibitor SgrT"
                     /protein_id="HBA7631189.1"
                     /translation="MRQFYQHYFTATAKLCWLRWLSVPQRLTMLEELIQWEGSHSDY"
     gene            complement(11708..12313)
                     /gene="leuD"
                     /locus_tag="J1Y83_003063"
     CDS             complement(11708..12313)
                     /gene="leuD"
                     /locus_tag="J1Y83_003063"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HBA7631190.1"
                     /translation="MAEKFIKHTGLVVPLDAANVDTDAIIPKQFLQKVTRTGFGAHLF
                     NDWRFLDEKGQQPNPDFVLNFPQYQWASILLARENFGCGSSREHAPWALTDYGFKVVI
                     APSFADIFYGNSFNNQLLPVKLSDAEVDELFALVKANPGIHFDVDLEAQEVKAGEKTY
                     RFTIDAFRRHCMMNGLDSIGLTLQHDDAIASYEEKQPAFMR"
     gene            complement(12324..13724)
                     /gene="leuC"
                     /locus_tag="J1Y83_003064"
     CDS             complement(12324..13724)
                     /gene="leuC"
                     /locus_tag="J1Y83_003064"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HBA7631191.1"
                     /translation="MAKTLYEKLFDAHVVYEAENETPLLYIDRHLVHEVTSPQAFDGL
                     RAHGRPVRQPGKTFATMDHNVSTQTKDINACGEMARIQMQELIKNCKEFGVELYDLNH
                     PYQGIVHVMGPEQGVTLPGMTIVCGDSHTATHGAFGALAFGIGTSEVEHVLATQTLKQ
                     GRAKTMKIEVQGKAAPGITAKDIVLAIIGKTGSAGGTGHVVEFCGEAIRDLSMEGRMT
                     LCNMAIEMGAKAGLVAPDETTFNYVKGRLHAPKGKDFDDAVAYWKTLQTDEGATFDTV
                     VTLQAEEISPQVTWGTNPGQVISVNDNIPDPASFADPVERASAEKALAYMGLKPGIPL
                     TEVAIDKVFIGSCTNSRIEDLRAAAEIAKGRKVAPGVQALVVPGSGPVKAQAEAEGLD
                     KIFIEAGFEWRLPGCSMCLAMNNDRLNPGERCASTSNRNFEGRQGRGGRTHLVSPAMA
                     AAAAVTGHFADIRNIK"
     gene            complement(13727..14818)
                     /gene="leuB"
                     /locus_tag="J1Y83_003065"
     CDS             complement(13727..14818)
                     /gene="leuB"
                     /locus_tag="J1Y83_003065"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HBA7631192.1"
                     /translation="MSKNYHIAVLPGDGIGPEVMTQALKVLDAVRNRFAMRITTSHYD
                     VGGAAIDNHGQPLPPATVEGCEQADAVLFGSVGGPKWEHLPPDQQPERGALLPLRKHF
                     KLFSNLRPAKLYQGLEAFCPLRADIAANGFDILCVRELTGGIYFGQPKGREGSGQYEK
                     AFDTEVYHRFEIERIARIAFESARKRRHKVTSIDKANVLQSSILWREIVNEIATEYPD
                     VELAHMYIDNATMQLIKDPSQFDVLLCSNLFGDILSDECAMITGSMGMLPSASLNEQG
                     FGLYEPAGGSAPDIAGKNIANPIAQILSLALLLRYSLDADDAASAIERAINRALEEGI
                     RTGDLARGAAAVSTDEMGDIIARYVAEGV"
     gene            complement(14818..16389)
                     /gene="leuA"
                     /locus_tag="J1Y83_003066"
     CDS             complement(14818..16389)
                     /gene="leuA"
                     /locus_tag="J1Y83_003066"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016831521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HBA7631193.1"
                     /translation="MSQQVIIFDTTLRDGEQALQASLSVKEKLQIALALERMGVDVME
                     VGFPVSSPGDFESVQTIARQVKNSRVCALARCVEKDIDVAAESLKVAEAFRIHTFIAT
                     SPMHIATKLRSTLDEVIERAIYMVKRARNYTDDVEFSCEDAGRTPIADLARVVEAAIN
                     AGATTINIPDTVGYTMPFEFAGIISGLYERVPNIDKAIISVHTHDDLGLAVGNSLAAV
                     HAGARQVEGAMNGIGERAGNCSLEEVIMAIKVRKDILNVHTAINHQEIWRTSQLVSQI
                     CNMPIPANKAIVGSGAFAHSSGIHQDGVLKNRENYEIMTPESIGLNQIQLNLTSRSGR
                     AAVKHRMDEMGYKESEYNLDNLYDAFLKLADKKGQVFDYDLEALAFIGKQQEEPEHFR
                     LDYFSVQSGSNDIATAAVKLACGEEVKAEAANGNGPVDAVYQAINRITDYNVELVKYS
                     LTAKGHGKDALGQVDIVANYNGRRFHGVGLATDIVESSAKAMVHVLNNIWRAAEVEKE
                     LQRKAQHNENNKETV"
     gene            complement(16481..16567)
                     /gene="leuL"
                     /locus_tag="J1Y83_003067"
     CDS             complement(16481..16567)
                     /gene="leuL"
                     /locus_tag="J1Y83_003067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leu operon leader peptide"
                     /protein_id="HBA7631194.1"
                     /translation="MTHIVRFIGLLLLNASSLRGRRVSGIQH"
BASE COUNT         3924 a         4178 c         4435 g         4294 t
ORIGIN      
        1 ttgcctgttt ttttcttgat aaaaatcccg ataacaattt cttttttagt gataataaaa
       61 tcaaaaagga agacttatgt atcgggatac ccgtcgtgaa catattgtcc ggttacttcg
      121 caacaaggat gagtgtagtg ttagcgattt agctaactat ttttctgtta ccaaagagac
      181 gattcggaca gatttagcgt ggttgcaaaa gcgtggtgta gtgagtcgtc atcatggagg
      241 cgtctcattg aaaaaacatt taatgcaaaa tgcgctttcg caacaagagt atgtcgatat
      301 gagtttgctt atcaagcaac aacagcaggg tattggctat ttaacctcgc ttgatgataa
      361 aggacgccga atggtaggta aagtttgtgt gttaggatca tttaacgtcg atattgtggc
      421 gaaagtgtat cgcttcccga atgaaggcga aacgttagtg gccagggaaa ctaccatggg
      481 gccggggggt aaaggcgcga atcaggcact agccgctcat cgggcaggtg cgcaaataca
      541 cttcgcctgt aagattggtc gcgatcagtt taatcatttt gcccgcaatc atattgagtc
      601 cattggtatt gattcattta ctttttatga gtctgaacta tctaccaccg gttgtgcggt
      661 catttatgta aatgatgaag gtgaaaacat gattgctatg tctccagggg caaatcatga
      721 attaagtgat aacgatatta ttcaactttc tcactttata gccgagtccg atgtattcat
      781 tgtacagatg gaaaataatc ttgccgctac tcaactggca ttaaaatgtg ctcaaaaaat
      841 gcaggtgaca acgattttga atcctgcacc gtggtccagt gatgtcgcca cattattacc
      901 ttttgttgat attgtgacgc caaatgaaac cgaagcatcg gcgatgtcag gtatggtaat
      961 tgacaatatt tctgatgctg tgaaggctgc gaagcatatt tataatgccg ggcaatgttc
     1021 agtaattatt acgatgggaa aacgaggtgc attaatttat gacgggcaac aatgtgcgca
     1081 tattccggca ttttcggcag tcgctgtaga tacaacaggt gcgggtgatg cgtttaatgg
     1141 cgcgctcgcc gccgcactgg ctaagggaga atcacttgtt aaagcagcct ggtatgccag
     1201 cgctttcgct tcgctggcag tagaaaaaga aggggcagcc aatatgcctg atatctctct
     1261 ggtggatgcc agaattcagc agcagaatgt tgccattcag actttctgat gtttcactga
     1321 acgaaggatg aaatatgaaa acaattgaag ggattattcc ggtaatgcta acgcctttta
     1381 cggcggataa taaaattgat tacctgagtc ttaaacggct aattgactgg tatatcgaaa
     1441 acggtgccga cgcgttattt gccgtgtgtc agtccagtga aatgcagtat ttaaccttag
     1501 aagagcgcgt ggagctggcg agctttgtgg ttgaatatag ccaacaacga gtaccggtta
     1561 ttgcgtccgg gcatatttct gatgatctgg aacagcaaat tcaggagctc aatgctatgg
     1621 cggcaacggg cattgatgcg ctggtgttag tgacgaatca tcttgatcca cataacgaag
     1681 gcaccacggt attcctccgt acgcttgatg cgctattgat gtcattaccc gaaaatatgg
     1741 cgctgggtct gtatgaatgt ccggccccgt atcgccgctt attaaccgat gaggaactgc
     1801 tttactgcgc acaaagcggg cgttttcaaa tcttaaaaga cgtcagttgt gatctaccta
     1861 cagtgacgcg acgggtgcaa ttaactcagc acacgccaat gacgattgtt aatgctaatg
     1921 ctgccattgc ctgggaggcg atgcttgcag gttcgaaagg tttttgtggc gtctttacca
     1981 actttcatcc tgacttgtat cactggcttt ggcttgaagg aaaacaacac cctgaagagg
     2041 caaagcaact cgcctggttt ttatcgctga gtgccgtcag tgaaaatatt ggctatccga
     2101 aaaatgccaa aatatttcat cagcagcgag gaacatttga ctgtgttcat tgccgggtaa
     2161 cggctgacga tgtgctgaaa aaatattggg ggctggagat ggtgctaaaa caaattgctg
     2221 atgccgcaga tttccagcgt caacaactca aatattaata tccgacagca cttgctgtta
     2281 ttaaaaaata attaagacag gagtctacaa atatgaaacg aaccctgatg agttgtgccg
     2341 ttgcttcact gctggtttca ggttccgcgc tggctgcatg gccagaaaaa ccaatccaaa
     2401 tcattgtccc ctgggcgcgg gaggaaatac cgatactgtt gcacgcctgg tggcagaagg
     2461 gctgcaaaaa gagttgaaag tgaatgtcaa cgtggtgaat cgcacgggag gtgctggggt
     2521 ggttggccat gatgccattg cgaaagctaa agcggatggc tataccctgg gaattgcgac
     2581 ggtagaaatc accatgatgc accatcaggg gatgacggcg ttaacgtatc agaactacac
     2641 gccaattaca cggctggcag tgaatctggg aggcgtgcaa gtcgcagcaa attcgcagtt
     2701 cgaaaatatc gtccagatga ccgactacat caaggcgaat ccaggtaagt taaaagcatc
     2761 gggtagcggg ttaaattccg gctggcatct aaacttgata ggtatgctcg acagtatggg
     2821 gcttccgcct tctgccgtga cctgggtgcc ttctgaaggt tccagttctg cactgatgga
     2881 actggtttcc ggtggtatag attttacaac gtcatcaccg ggcgaagcga aaagtatggt
     2941 cgatgccaaa atggtgcgta atttagcgac catggcagaa aaatcggcag ggctttataa
     3001 agatcttcca gtattccaac aagcaactgg ctataaatat catttggcga catggaatgc
     3061 cttagtggtt ccggcgggta ctcctgaaga ggtctcacaa aaattaactg ccgctatgca
     3121 ggcagtgttt gcgcagggga aattacaaga attcgctacc aaacagggtt ttgaagttta
     3181 cccgctgtat ggcaaagaga tgacgacatt tatggcagat gaagatgcca gatacggaaa
     3241 attaataacc aaaatcaaag gttaatcaga cagggcggtg aatatatcgc cgccaaatta
     3301 cagggatagc attatgcgca taataccggt aacgccgtta tttctgctga tttttgccat
     3361 gtttattttt ggctggagca taacgcaata tggaggcata tcgcagtatg gcgctggatt
     3421 tatgccttgc atcatcagtg gattgttgtt gtttttagcg gctattgatg tagcgataga
     3481 tattaaacac cggcaccgta acaaagtttc tttaacgctt gatgaagtga aagcactttt
     3541 actggttatt acagtggtac tgctgtttgt attattgtta gatattctcg gttttgtaat
     3601 ttgctcattt ttgttgcttt ttattttaat gaaaatacga ggtgtcagac ttttgcattc
     3661 cgttatcgtt tcgatatcgg catctcttat tatctggttt atttttgcca gagtgttgtt
     3721 ggtcgccttt cctgaaggaa tcttgtttta aggatgcaat atggatattt tgattcaggc
     3781 actaacgtat attaatttga atgccattct ggccattatt gctgctacgg cctttgggtt
     3841 atttgtggga gccattccag gattgaccgc aacgatggcg gttgcgctaa tggtaccttt
     3901 taccttcttt cttgatccta ttcccgctct ggcattaatg attgccgttg gtgcgtcatc
     3961 catttatgcc ggagatatcc ccggcgcact tttgcgtata ccagggacac ctgcttcggc
     4021 ggcttatgtt gctgattcaa atgaactggt aaagcaaggt aaggtgaatc gcgtattggg
     4081 acttgggctg gtgagttcgg tcattggtgg cgtgattgga tcacttattt tgatgttggc
     4141 tgcgcctgta ctggcgaaat ttgcccttaa atttagttct tatgaataca catggctttc
     4201 tcttttaggc ttaacctgcg cgactctggt tgtggggaat caaccactga aaggcatggt
     4261 gaccttatgt ttgggattat tactggcgtg tatcggttat gatcaaatat ccggggtccc
     4321 acgttttacg tttgggcaaa tcagcctgtt acaaggggtg aactttattc ctgcgatgat
     4381 cggtctgttc gcaatatccg gcgcaattga atattatcgt gacaaaatta gccaggtaag
     4441 tggtgctgtg ccacaaacac aaacggggat taatttattc aagggaatgg ggcaggtgat
     4501 ttggtcacga aaattaaatc ttggccgtag cagtattatc ggtacgctta tcggtgcatt
     4561 acccggcgct ggagcagata ttgctgcgtg gataagttac gccatttcaa aaaaattctc
     4621 tcgccagcag gagttatatg ggcatggttc tgaggaggcg attgttgatt cctcgagcag
     4681 taacaatgcc agtctggcgg ggagttggat cccttcatta gttttcggta ttccgggcga
     4741 ttctgccgcc gcaattatca tcggcgtgct ctatatgaag gatatgaatc caggaccgac
     4801 attatttctg ttccaggcag ataaactcta tgccgtgttt atcttgtttc tgatcgccaa
     4861 tattgcattg ctaccgttag ccacaattgc tgtcagtttt atcaaacgca tcatctggat
     4921 tgataaagcg attctgtacc cgattattct gattttcagt attgtcggtg cgtttgctat
     4981 cgataactcg ggtgcctctg tcgttgtcat gttggtgatg ggggttcttg gatactggtt
     5041 acaacgtaag gaatacccgg tttcgcccat tattttgggg atgattcttg ggccaatgtt
     5101 ggaaaaaaac ttattatctt caatgattaa gtctaatggc gaatggctgg cgtttgttga
     5161 gcgcccggta tctatggcac tggctgcctg tttctttttg gttgtcgcgt tgcaaggacg
     5221 caatatctac cgttcattta gccgttaatc cgctgttttc agaccatcca tgttgtggat
     5281 ggtctgcctt cccctaagta aaatcctgcg tgactgctaa atttatactc taaatgattc
     5341 gcgttgcagg agtatattca tacaaagaat agattgacct gaataccacc acttcgcaca
     5401 atccacgacg aattcatcaa ccgggcgcat tatgcaagca ttgctggaac actttattac
     5461 ccaatccacc gtgtattcat tgatggcggt ggtgttggtg gcctttctgg agtcgctggc
     5521 gctggtcggt ttgattctac ccggtacggt gctgatggcg gggctgggtg cgctgattgg
     5581 cagtggcgag ttaagtttct ggcacgcctg gctggcaggg atcgtcggtt gcctgctggg
     5641 cgactggatc tctttctggc tgggctggcg ttttaaaaag ccattgcatc gctggtcatt
     5701 tctgaagaaa aacaaagcat tactcgataa aactgaacat gcgttgcatc aacacagcat
     5761 gttcaccatt ctggtcggtc gctttgttgg cccgacgcgt ccgctggtgc caatggtggc
     5821 gggaatgctg gatctgccgg tggcaaaatt cattacgccg aatattatcg gctgcctgct
     5881 gtggccaccg ttttacttcc tgccagggat tctggcgggt gcggcgatcg atattcctgc
     5941 cgggatgcag agcggtgagt ttaaatggtt gctgctggca acagcggtgt ttttgtgggt
     6001 tggaggctgg ctgtgctggc ggttatggcg cagcggtaaa gcgactgacc gtttgagtca
     6061 ttatttgtcc cgcggtcgtt tgttgtggct gacgccgttg atttctgcca tcggcgtggt
     6121 ggcgctggtg gtgttaattc gccacccgtt gatgccggtg tatatcgata ttttgcgtaa
     6181 agtggttggg gtttagggga tagccttgtg cgggttgcct gatgcgacgc ttgccgcgtc
     6241 ttatcaggcc tgcaaaacgc actacccgta ggtcggataa ggcgttcacg ccgcatccga
     6301 cagtgcatat taacctttaa ttcccaacag cgccgaagca ctcgccttac cgctcaacaa
     6361 ctcatcggtc ttaccctgcc aggcgatacg cccgtcggcg accaccaccg agcgcgtggc
     6421 gatccgcgcc gcatcttcca cactgtgcga caccatcaag agcgtcattt tttgctgttg
     6481 gcagctcgtg ctcaccagcg tcagcatctc ctgacgtagc gccggatcga gtgcggaaaa
     6541 cggttcatcg agcaataaaa tgggctgttc gcgtaccaga caacgcgcta acgccacgcg
     6601 ctgtcgctga ccgccggaaa gctcgccagg taaccgcgcc attaaattat caatccccat
     6661 ctggtgggca atcgcgtgca ttttcttctg ctgcgccgca tttagtttca accccggatt
     6721 tagccccagc ccgatgtttt gcgcgaccgt cagatggctg aataggttgt tctcctgaaa
     6781 cagcatcgac accggacggc gtgacggcgg cgttgtggtg tgatctacgc cgtcgatagt
     6841 cagcaaaccg ctggctggcg tcagaaaacc ggcgatcaaa tttagcaggg tgcttttacc
     6901 cgcgccgctt ggcccgagga tcgccaattg ctcgccgcgt tccaccgtta agctaaaacg
     6961 catcggcaaa tggtggtaaa gccaggtgat atcagtcagt tttaacattt cgccccggta
     7021 ttttttcaat cacggtaaac agcagaaaac agagcagcag cagaattaac gcggtgaccg
     7081 cgccgtcctg gctgcgatag gagccaattt gctggtagag ataaaacggc agggtacgga
     7141 aatcatcgtt accgaacaat gccaccacgc caaaatcacc aatcgacagc acacaagcaa
     7201 aggccagcgc ctgcgccagt ggacgtttca gggcgcgcag ctccaccact tttaagcgtg
     7261 accagccttc aatgcccagc gactgacaca acatgctgta gcgggcggtg atatcgcgca
     7321 tcgggttttc cagtactttc agcgcataag ggattgccat taacgcattg gtgaaaatca
     7381 caatgccgtc agcagattgt ggcaggccga tagtgttgtt gagcagtaaa aagaagcccg
     7441 tagccagcac aatccccggc atggcgagga tcaacatccc gctcatctcc agcgcctgac
     7501 ccgccagcat tttctgccgc gcccgaagtt cgcgactgct ccatagcagc atcatggtca
     7561 gcactacgca caatacgcct gccgccagcg caatacgcaa cgaggtccac agcgcctgcc
     7621 acagcaccgg ttgtgccagc acttccggca actggcgatt taccccatcg acgatcaccg
     7681 ccagtaacgg tggcagcaac agcagcagcg ccagcacaat taacgccgtg tcgcaaatgc
     7741 gactatgcag acgatcgtcc gggtcgcgcc agccttgcag cagtgtggta ccaggcgcaa
     7801 tggccttact caatcgctga ctcaacagca ccagcccgag gcagcacacc atctggatca
     7861 gcgccagcat tgccgcgcgg gcaggatcgt agtcgtaact cagcgcctga taaattgcca
     7921 gctcgatagt ggtcgcctgc ggaccgccgc ccagcgatag cacggtggcg aagctggcga
     7981 aacagagcat aaagattagt gcggcaaccg gcgggatttg tcgccgtaac cacggccatt
     8041 cgacgaagcg gaagaagtga cagccgcgca tcccaagctg ggcggcaagc tgacgttgtt
     8101 cgccgggaat gttttccagt gcctggagta ataaacggct agccatcggc agattaaaaa
     8161 acacatgggc cagcaaaatg ccttgcaggc cgtagggcga aaaggtccac tccagaccaa
     8221 gtgattgcca gagcgaggcc agccagccct ggcgaccata gacgctaaga atgccgaaaa
     8281 cggcaaccag caccgggagg atcaaggtca ttgcgcacag acgcaacagc atctgtcgcc
     8341 caggaaagcg cctgcgatag agggcgcggg cgaggaatat tgcgggtacg acagagagct
     8401 gcgccgagag aaacgcctgc cagaaggaga agcgcaccac atgccacagg tagctgtcct
     8461 gccagactgc cacccagtca ccctgcggcg cgttccacca cagggcgaga aacgccgcca
     8521 gtgcaaccgc taccaccagc gtggcggcgc ttacgcctgg aattaaccag ccgggaatta
     8581 acggctgacg gcgcgttgcc attcgctaat ccatgcctga cgttgtgccg ccacttcggc
     8641 gggtgtgaac tctagcgtgg tggctggttt ggtcaattgt tcaaatcctg caggcagagt
     8701 gacgtttgct accggataca tccagttgcc ggttgggatc gcattctgaa aagccggaga
     8761 aaccataaac tggaggaatt tttgcgccag ctctggctgc ttgctggcag cggtgcgggc
     8821 ggcaacttcc acctgcagat agtgaccttc gctaaagttt gtggcggcgt agttatcttt
     8881 cttctcttcg agaatgtgat aagccggaga ggtggtgtaa ctcagtacca gatcgctttc
     8941 gccttttaaa aacaggccgt aggcttcgct ccagcctttg gtgaccgtga cagttttctt
     9001 cgccagtttc tgccaggctt gcggggcgtt atcgccatag actttttgca tccatagcaa
     9061 cagacccaga cctggtgtac tggtacgcgg atcttcataa atcacccgcc agttttgatc
     9121 gctctcaacc aattctttca ggctttgcgg cggatttttc agtttgttct tgtcatagac
     9181 gaaggcgaaa tagccgtaat caaacggtac gaaagtgtca ttattccagc cgccaggaac
     9241 gttaacggct tccgctgcca caccgctttt agcaaacagc cctgttttgc tggcggcgtc
     9301 taacagattg ttatccagcc ccagcaccac atcggctttg ctgtttttgc cttccatccg
     9361 tagacggttg agcagcgaaa cgccatcttc cagcgccacc agtttcagtt cgcaattaca
     9421 gtctgcttca aaggcttttt tgactaccgg accaggtccc cagtcagcgg caaaggaatc
     9481 gtaggtataa acagtcagaa caggtttagc gaaaacaggc gctgtgcaca gcaacagcag
     9541 gggaagacat ttttttaaca ctttgcacct cacaaaagag tggcaaagga cttgagaagg
     9601 agcctcaaat cccttcgccg gcgttatccg gatcaggttc gacgggtatt ttctcagcgc
     9661 acgcggctgc gtggcacccc gttgagaacg gcgttagtgt agtgattttg ttatcaacca
     9721 gcaatcatgg atccggtggt gcaaaccacg ctgatttaaa atcgaaccag ccgagggtgt
     9781 tcatgcgcag gccgcgcata ctacgttgcc cctgaatgat cagccagtgg tggataagtg
     9841 gcaccattgc tttgctggcg accagttgct ggcaccagtt cgccaggttc atttcgccat
     9901 tgcgccagcg ggcggcgtcg gcttgccagt cgatcggaat acagtgttgg agcagcggca
     9961 cctcgcacaa gtgcgcgaac agcgaaaaat ccagcggcag cgtaaagttg gcgctgttaa
    10021 gccagatatc gctctcgatc tctccttcat gccactgatc gtagctgatc tctttgattt
    10081 tcagcgtgac ctggtgactt gccagaatct gctgcatgat cccggcaatc acccgatgtt
    10141 caatatgatc ctgataaaag gtcagggtaa ggttttccag ccccgcgggt ttttcgcaat
    10201 gtgtcgggcg ggcatggtgc caacggggga gcagtccata agccgggaac cacagttgct
    10261 ggtactgttc ctcagcgaaa tagaccagat tcgtcggaga aagcacatag cttacccagt
    10321 ctctgacttg ctgattcgcc cctcgatggg tgcggctgtc gaacagtaaa tagtagcaac
    10381 cttcctccag gcggctttca atctcttttt cctcgccctg tggacctttt agcatcagcc
    10441 ctccggctgg ctcgtcggca atttccggca gaacccagac gttaacttcg tcgattaatg
    10501 cccggtaacc gaagaagtca tcgaatgcct gaattttcag ttgattggtg ctgttgcgga
    10561 tcaccgcata cggaccggtg ccgatgggat ggctggcaaa gttactgagg gtttcccatt
    10621 cgcgcggcag gatcattgcc ggaacttgcc ccagcagtaa cggcaaccag cggtccggtt
    10681 gcgtgagatg gatatccagc gtccagggcg tcggcgacac aatgtcagca atatgcgaat
    10741 agagcggaag cgtattgatt cgttttaaag aggagatcac atcgtccatt tccagttcac
    10801 gaccatggtg aaaatggaca cctggacgca aaaagaaacg ccagtgaagc ggtgatattt
    10861 gctgccagtg gtgggcgatg tctgcttcca gttccccatt ttcctcattt atgcgcgtta
    10921 gcgaactgaa gatttgccgg gcgatatggg tttcagaacg gcgcaatgcg ctgccaggta
    10981 gcagattacg caacggacga tagtagagca cgcgcaggat gtgccgcccc tggcggaagc
    11041 tgcggcccag atgagaaacc agcatttgcc gcacagtcgc tttgtcgcca accaactgca
    11101 ctagttggtc gatacgatcc tgctccagca ggtcttccgc ccgctgttgc tgaagcgcca
    11161 gcccggtata gaggaatgtc aggcgtgaac gtttaccgcg cccgacttcc gcttcccacg
    11221 tcagccagcc gcgatcctgc atggtgttga gcagggtgcg catatgacga cgcgagcaac
    11281 tcagtaacgc tgccagttcg ttgagcgtgg tctcctgaga tttaccctcg cagcattgcc
    11341 acaggcggat gaactgttgt tgcagacgag cagatggcat aaaaggggaa ctcctgcgca
    11401 aaagacagca attttatttt ccctatatta agtcaataat tcctaacgat gaagcaagga
    11461 ggtgccccat gcgtcagttt tatcagcact attttaccgc gacagcgaag ttgtgctggt
    11521 tgcgttggtt aagcgtccca caacgattaa ccatgcttga agagctgata cagtgggagg
    11581 ggagtcattc tgactactga tttgcggaca tcatgtgtga ctgagtattg gtgttaatcg
    11641 ccacgccagc agtgattatc tgctggcttt tttctttttg gcgattgagt gccggacggt
    11701 gtacaaacta acgcataaac gccggttgct tctcttcata agacgcaata gcatcgtcgt
    11761 gctgcagagt caggccgatg ctgtccagac cgttcatcat gcagtggcgg cggaaggcat
    11821 cgatggtaaa gcgataagtt ttctctcccg ctttcacctc ttgcgcttcc aggtctacat
    11881 caaaatgaat ccccggattg gctttcacca gcgcaaacag ctcatccact tctgcatcgc
    11941 ttaatttcac cggcagcagc tggttgttaa agctattgcc gtagaagatg tcagcaaaac
    12001 tcggcgcaat caccacctta aaaccgtaat cggtcaatgc ccacggcgcg tgctcacgcg
    12061 aggaaccgca gccgaagttt tctcgtgcca gcaaaatgga agcccactga tactgcggga
    12121 agttcagcac gaagtccggg tttggctgtt ggcctttttc atccagaaaa cgccagtcgt
    12181 taaacagatg cgcgccaaaa cccgtacggg tcactttctg caaaaactgt ttcgggatga
    12241 ttgcatcggt atcgacattg gcggcatcca acggaaccac caggcctgtg tgtttgataa
    12301 atttctctgc catggtgtgc tccttattta atgttgcgaa tgtcggcaaa atgaccggtc
    12361 actgccgcgg cggcagccat tgccgggctg accagatgcg tgcgcccgcc gcgcccctgg
    12421 cggccttcaa agttacggtt gctggtggag gcgcaacgtt cgcccggatt caggcggtcg
    12481 ttgttcatcg ccagacacat tgaacaacct ggcaagcgcc attcaaaacc agcttcgata
    12541 aaaattttgt ccagaccttc cgcttccgcc tgggctttaa ccggaccgga gccgggaacc
    12601 accagcgctt gtacgcctgg cgcgactttc cggcctttgg cgatttccgc tgccgcgcgt
    12661 aaatcttcaa tacgcgagtt agtacaggaa ccaataaaca ctttgtcgat agccacttct
    12721 gtcagcggaa tacccggttt cagccccata taggccagcg ctttttctgc cgacgcgcgt
    12781 tcaaccggat cggcaaacga agccggatcg ggaatattgt cgttcacaga aatcacctgg
    12841 cctggattgg tgccccaggt gacctgcggc gaaatctctt ctgcttgcag agtgacaacg
    12901 gtatcgaaag ttgctccttc gtcggtttgc agggttttcc agtaggcaac ggcgtcgtcg
    12961 aaatctttgc ctttcggcgc atgcagacgg cctttaacat agttaaaggt ggtttcgtcc
    13021 ggtgcaacca gaccggcttt tgcgcccatt tcgattgcca tattgcacag ggtcatacga
    13081 ccttccatgc ttaaatcacg gattgcttcg ccgcaaaatt ccaccacatg cccggtaccg
    13141 cctgcgctgc cggttttacc aataattgcc agcacgatat cttttgcggt aatgcccggc
    13201 gcggctttgc cctggacttc aattttcatg gtcttcgccc ggccctgttt cagggtttgc
    13261 gttgccagta cgtgttcaac ttcggaagtg ccgataccaa aggccagtgc gccaaacgcg
    13321 ccgtgggtgg cggtatgtga gtcgccgcag acaatggtca tccccggcaa ggtcacgccc
    13381 tgttccggcc ccattacgtg gacgatcccc tgatacgggt gattcaggtc atacagctcg
    13441 acgccaaatt ctttgcagtt tttgatcagc tcctgcatct ggatgcgcgc catttcaccg
    13501 caggcattaa tgtctttcgt ctgggtagag acgttgtgat ccatggtggc gaaggttttg
    13561 cccggctgac gtaccgggcg accgtgggcg cgcagaccat cgaacgcctg cggtgaggtc
    13621 acttcatgca ccaggtggcg gtcgatatat aacagcgggg tttcgttttc ggcttcgtac
    13681 actacgtgag cgtcgaataa tttttcgtat aacgtcttag ccatgattac accccttctg
    13741 ccacatagcg ggcaatgata tcgcccattt catcggtact aacggcggca gcgccacggg
    13801 ctaaatcccc ggtgcgaatg ccttcttcta atgcgcggtt aatggcgcgt tcaatggcgg
    13861 aagccgcatc atcggcatcc aggctgtaac gcagcagcag cgccagcgac agaatttgtg
    13921 caatcgggtt ggcgatattt ttacctgcga tatcgggtgc cgagccgccc gccggttcat
    13981 acagaccaaa accttgctcg ttcaggctgg cggaaggcaa catccccatt gagccagtga
    14041 tcattgcgca ctcatcagac agaatgtcgc caaacaggtt ggagcacagc aagacgtcaa
    14101 actgtgatgg atctttaatc agctgcatgg tggcgttgtc gatgtacata tgcgccagtt
    14161 cgacgtccgg gtattccgtg gcgatctcgt taacgatctc ccgccataaa atagaggatt
    14221 gcagcacgtt ggctttgtcg attgaggtga ctttgtggcg acgcttgcgg gctgattcaa
    14281 atgcgatgcg ggcaatacgc tcgatctcaa aacggtggta cacctcggta tcaaacgctt
    14341 tttcatattg tccgctacct tcgcggcctt ttggctgacc gaaatagatg ccgccggtca
    14401 gttcgcgcac acacaggatg tcaaagccgt ttgcggcaat gtcagcacgc agcggacaga
    14461 atgcttccag cccctgatac agttttgccg gacgcagatt gctgaataat ttgaagtgct
    14521 tacgcaaagg cagcagtgcg ccgcgttctg gttgctggtc tggcggcaaa tgttcccact
    14581 tcggaccacc taccgagcca aacagcacgg catcggcttg ctcacaacct tcaaccgtcg
    14641 caggcggcag cggctgcccg tggttatcaa tggctgcgcc gcctacatcg taatggctgg
    14701 tggtgatacg catcgcaaat cggttgcgca cggcatccag cactttcagc gcctgggtca
    14761 tcacttccgg accaataccg tcccccggca atacggcaat atggtaattc ttcgacatca
    14821 cacggtttcc ttgttgtttt cgttgtgttg agctttgcgt tgcaactctt tttcgacttc
    14881 tgcggcacgc cagatattgt tcagtacgtg caccatggct ttggcggagg actcgacaat
    14941 atcggtggcc aggccgacgc cgtggaagcg gcgaccgttg tagttggcga caatatccac
    15001 ctgacccagc gcatctttac cgtggccttt ggcggtcagg ctgtatttca ccagttcgac
    15061 gttatagtca gtgatgcggt ttatcgcctg gtagacggca tcgaccggac cgttaccgtt
    15121 ggcggcttct gctttgactt cttcgccaca ggccagtttg acggcggcag tggcaatatc
    15181 gttagaacct gactgcacgc tgaagtaatc cagacggaaa tgctccggct cttcttgctg
    15241 cttaccgatg aaggccagcg cctccagatc gtaatcaaac acctggcctt ttttgtccgc
    15301 cagcttcagg aaagcgtcgt acaggttgtc taaattatat tcactttctt tatatcccat
    15361 ctcatccatg cgatgtttca ccgccgcacg ccccgaacga gaggtcagat tcagctggat
    15421 ttggttcaga ccaatagatt ctggtgtcat gatttcgtag ttttcgcggt ttttcagtac
    15481 accatcctgg tggataccgg aggagtgtgc gaatgcgccg ctgccaacaa tggctttgtt
    15541 tgccgggatc ggcatattac aaatctggct aactaactgg ctggtgcgcc atatctcctg
    15601 gtgattgatg gcggtgtgga cgttgagaat atccttacga actttgatcg ccatgatgac
    15661 ttcttcaagg gaacagttac cggcacgctc gccgatcccg ttcattgcgc cttctacctg
    15721 ccgtgctccg gcatgtaccg ccgccagtga gttgccgacc gccaggccca aatcgtcgtg
    15781 ggtatgtaca gaaataatgg ctttgtcgat gttgggtacg cgttcataca ggccgctgat
    15841 gattccggcg aactcaaatg gcatggtgta gcccacggtg tccggaatgt tgatggtggt
    15901 ggcaccggca ttaatcgccg cttcgaccac tcgcgccaga tcggcaattg gggtgcgtcc
    15961 ggcatcttcg caagaaaatt caacatcatc ggtgtaatta cgggcgcgtt tcaccatata
    16021 gatagcgcgt tcgatcactt cgtccagcgt gctgcgcagc ttggtggcga tgtgcattgg
    16081 cgaagtggca ataaaggtat gaatacggaa ggcttcggcg actttcaggg attcggctgc
    16141 cacgtcaata tctttttcca cgcagcgagc taacgcgcat acacggctgt ttttaacctg
    16201 gcgagcgatg gtctgcactg attcaaaatc acccggcgaa gagacgggga aaccgacttc
    16261 catcacgtca acacccatac gctcaagggc cagcgcaatt tgcagttttt ctttcacact
    16321 caagcttgcc tgtaacgcct gttcaccgtc gcgcaatgtg gtatcgaaaa taatgacttg
    16381 ctggctcatg gtttgggtcc ttgtctcttt ttgagcgcct cgcttcgggc ataaaaaaac
    16441 ccgcgcaatg gcgcgggttt ttgtttgact gcgtgctggc ttaatgctgg atgccgctca
    16501 ctcgtctacc gcgcaaagaa gatgcgttta gtagtagtag accgataaag cgaacgatgt
    16561 gagtcattaa atcagctcca gatgaatgcg atatgctttt agagttactg gatacaaaaa
    16621 cggatgtcaa ccctgaagca ataaaaacgt cacgccattg tgagttcggc atccgtaaaa
    16681 ttagctaatt gtgctgcggt ggttaaaaca agcgatatta atttctgctt aaccgccgat
    16741 gcttttcatc ggttgacata tttcagaata attttttgca tctaatcaac gagggaaagg
    16801 gggataaaat gcacgcgtta caaaacctat c
//
LOCUS       DADSYM010000107        16647 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2415
            SAMN08039579-rid11723173.denovo.107, whole genome shotgun sequence.
ACCESSION   DADSYM010000107 DADSYM010000000
VERSION     DADSYM010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039579
            Sequence Read Archive: SRR6362533
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16647)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16647)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980162.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 03:39:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,484
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 4,347
            CDSs (with protein)               :: 4,347
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 43 of 85
            Pseudo Genes (incomplete)         :: 37 of 85
            Pseudo Genes (internal stop)      :: 19 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16647
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039579-rid11723173.denovo.107"
                     /strain="LGE2415"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8302 N 95.9628 W"
                     /collection_date="2015-08-24"
     gene            104..466
                     /locus_tag="J1Y83_003068"
     CDS             104..466
                     /locus_tag="J1Y83_003068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA7631195.1"
                     /translation="MNEFEKIFNEMNLDRALLPILFRSNRSTVWKYLSGDSTAPASAM
                     SLIMLLQLIQKRNPDLLAEWLTLSDFTIPPEVYLDQPDYWKGWVYTQHKVNKNVLEYL
                     KKHYPDEDQKSMGKGREE"
     gene            complement(569..1027)
                     /locus_tag="J1Y83_003069"
     CDS             complement(569..1027)
                     /locus_tag="J1Y83_003069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HBA7631196.1"
                     /translation="MMELMVVHPHIFWLSLGGLLLAAEMLGGNGYLLWSGVAAVITGL
                     VVWLLPLGWEWQGVMFAVLTLLAAWLWWKWLSRRVREQKHSDSHLNQRGQQLIGRRFV
                     LESPLVNGRGHMRVGDSSWPVSASEDLGAGTHVEVIAIEGITLIIRAVIA"
     gene            complement(1024..1941)
                     /locus_tag="J1Y83_003070"
     CDS             complement(1024..1941)
                     /locus_tag="J1Y83_003070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH/Band 7/PHB domain protein"
                     /protein_id="HBA7631197.1"
                     /translation="MLIFIPILIFVALVIVGAGVKIVPQGYQWTVERFGRYTKTLQPG
                     LSLVVPFMDRIGRKINMMEQVLDIPSQEVISKDNANVTIDAVCFIQVIDAPRAAYEVS
                     NLELAIINLTMTNIRTVLGSMELDEMLSQRDSINSRLLHIVDEATNPWGIKVTRIEIR
                     DVRPPAELISSMNAQMKAERTKRAYILEAEGIRQAEILKAEGEKQSQILKAEGERQSA
                     FLQAEARERSAEAEARATKMVSEAIASGDIQAVNYFVAQKYTEALQQIGSSSNSKVVM
                     MPLEASSLMGSIAGIAELVKDSANKRTQP"
     gene            2087..2764
                     /gene="fetA"
                     /locus_tag="J1Y83_003071"
     CDS             2087..2764
                     /gene="fetA"
                     /locus_tag="J1Y83_003071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter ATP-binding protein FetA"
                     /protein_id="HBA7631198.1"
                     /translation="MQENSPLLQLQNVGYLAGDTKILNNINFSLCAGEFKLITGPSGC
                     GKSTLLKIVASLISPTSGTLLFEGEDVSTLKPEIYRQQVSYCAQTPTLFGDTVYDNLI
                     FPWQIRNQQPDPAIFLDFLERFALPDSILTKNIAELSGGEKQRISLIRNLQFMPKVLL
                     LDEITSALDESNKHNVNEMIHRYVREQNIAVLWVTHDKDEINHADKVITLQPHAGEMQ
                     EARYELA"
     gene            2751..3530
                     /gene="fetB"
                     /locus_tag="J1Y83_003072"
     CDS             2751..3530
                     /gene="fetB"
                     /locus_tag="J1Y83_003072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron export ABC transporter permease subunit
                     FetB"
                     /protein_id="HBA7631199.1"
                     /translation="MNSHNITNESLALALMLVVVAILISHKEKLALEKDILWSVGRAI
                     IQLIIVGYVLKYIFSVDDACLTLLMVLFICFNAAWNAQKRSKYIAKAFISSFIAITVG
                     AGITLAVLILSGSIEFIPMQVIPIAGMIAGNAMVAVGLCYNNLGQRVISEQQQIQEKL
                     SLGATPKQASAILIRDSIRAALIPTVDSAKTVGLVSLPGMMSGLIFAGIDPVKAIKYQ
                     IMVTFMLLSTASLSTIIACYLTYRKFYNSRHQLVVTQLKKK"
     gene            complement(3593..4447)
                     /gene="cnoX"
                     /locus_tag="J1Y83_003073"
     CDS             complement(3593..4447)
                     /gene="cnoX"
                     /locus_tag="J1Y83_003073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperedoxin"
                     /protein_id="HBA7631200.1"
                     /translation="MSVENIVNINESNLQQVLEQSMTTPVLFYFWSERSQHCLQLTPI
                     LENLAAQYNGQFILAKLDCDAEQMIAAQFGLRAIPTVYLFQNGQPVDGFQGPQPEEAI
                     RSLLDKVLPREEELKAQQAMQLMQEGNYTDALPLLKDAWQLSNQNGEIGLLLAETLIA
                     LNRSEDAEAVLKTIPLQDQDTRYQGLVAQIELLKQAADTPEIQQLQQQVAENPEDAAL
                     ATQLALQLHQVGRNEEALELLFGHLRKDLTAADGQTRKTFQEILAALGTGDALASKYR
                     RQLYALLY"
     gene            complement(4508..5317)
                     /gene="ybbO"
                     /locus_tag="J1Y83_003074"
     CDS             complement(4508..5317)
                     /gene="ybbO"
                     /locus_tag="J1Y83_003074"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(+)-dependent aldehyde reductase"
                     /protein_id="HBA7631201.1"
                     /translation="MTHKATEILTGKVMQKSVLITGCSSGIGLESALELKRQGFHVLA
                     GCRKPDDVERMNNMGFTGVLIDLDLPESVDRAADEVIALTDNCLYGIFNNAGFGMYGP
                     LSTISRAQMEQQFSANFFGAHQLTMRLLPAMLPHGEGRIVMTSSVMGLISTPGRGAYA
                     ASKYALEAWSDALRMELRHSGIKVSLIEPGPIRTRFTDNVNQTQSDKPVENPGIAARF
                     TLGPEAVVDKVRHAFISEKPKMRYPVTLVTWAVMVLKRLLPGRVMDKILQG"
     gene            complement(5307..5930)
                     /gene="tesA"
                     /locus_tag="J1Y83_003075"
     CDS             complement(5307..5930)
                     /gene="tesA"
                     /locus_tag="J1Y83_003075"
                     /EC_number="3.1.1.5"
                     /EC_number="3.1.2.14"
                     /EC_number="3.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005225520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional acyl-CoA thioesterase I/protease
                     I/lysophospholipase L1"
                     /protein_id="HBA7631202.1"
                     /translation="MNFNNVFRWHLPFLFLVLLTFRAAAADTLLILGDSLSAGYRMSA
                     SAAWPALLNDKWQSKTSVVNASISGDTSQQGLARLPALLKQHQPRWVLVELGGNDGLR
                     GFQPQQTEQTLRQILQDVKAANAEPLLMQIRLPANYGRRYNEAFSAIYPKLAKEFDVP
                     LLPFFMEEVYLKPQWMQDDGIHPNRDAQPFIADWMAKQLQLLVNHDS"
     gene            5901..6587
                     /locus_tag="J1Y83_003076"
     CDS             5901..6587
                     /locus_tag="J1Y83_003076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001110574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBA7631203.1"
                     /translation="MPAENIVEVHHLKKSVGQGEHELSILTGVELVVKRGETIALVGE
                     SGSGKSTLLAILAGLDDGSSGEVRLVGQPLHNMDEEARAKLRAKHVGFVFQSFMLIPT
                     LNALENVELPALLRGESSAESRNGAKALLEQLGLGKRLDHLPAQLSGGEQQRVALARA
                     FNGRPDVLFADEPTGNLDRQTGDKIADLLFSLNREHGTTLIMVTHDLQLAARCDRCLR
                     LVNGQLQEET"
     gene            6584..8998
                     /locus_tag="J1Y83_003077"
     CDS             6584..8998
                     /locus_tag="J1Y83_003077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBA7631204.1"
                     /translation="MIARWFWREWRSPSLLIVWLALSLAVACVLALGNISDRMEKGLS
                     QQSREFMAGDRALRSSREVPQAWLEEAQKRGLKVGKQLTFATMTFAGDTPQLANVKAV
                     DDIYPMYGELQTNPPGLKPQAGSVLLAPRLMALLNLKTGDTIDVGDATLRIAGEVIQE
                     PDSGFNPFQMAPRLMMNLADVDKTGAVQPGSRVTWRYKFGGSENQLDGYEKWLLPQLK
                     PEQRWYGLEQDEGALGRSMERSQQFLLLSALLTLLLAVAAVAVAMNHYCRSRYDLVAI
                     LKTLGAGRAQLRKLIVGQWLMVLALSAVTGGAIGLLFENVLMVLLKPVLPAALPPASL
                     WPWLWALGTMTVISLLVGLRPYRLLLATQPLRVLRNDVVANVWPLKFYLPIVSVVVVL
                     LLAGLMGGSMLLWAVLAGAVVLAFLCGVLGWMLLNVLRRMTLKSLPLRLAVSRLLRQP
                     WSTLSQLSAFSLSFMLLALLLVLRGDLLDRWQQQLPPESPNYFLINIATEQVTPLKTF
                     LAEHQIVPESFYPVVRARLTAINGKPTEGNEDEALNRELNLTWQNTRPDHNPIVAGNW
                     PPKADEVSMEEGLAKRLNVALGDTVTFMGDTQEFRAKVTSLRKVDWESLRPNFYFIFP
                     EGALDGQPQSWLTSFRWENGNGMLTQLNRQFPTISLLDIGAILKQVGQVLEQVSRALE
                     VMVVLVTACGMLLLLAQVQVGMRQRHQELVVWRTLGAGKKLLRTTLWCEFAMLGFVSG
                     LVAAIGAETALAVLQSKVFDFPWEPDWRLWIVLPCSGALLLSLCGGWLGARLLKGKAL
                     FRQFAG"
     gene            complement(9239..10333)
                     /gene="mnmH"
                     /locus_tag="J1Y83_003078"
     CDS             complement(9239..10333)
                     /gene="mnmH"
                     /locus_tag="J1Y83_003078"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-selenouridine(34) synthase MnmH"
                     /protein_id="HBA7631205.1"
                     /translation="MQERHTEQDYRALLIADTPIIDVRAPIEFEQGAMPAAINLPLMN
                     NDERAAVGICYKQQGSDAALALGHKLVAGEIRQQRMDAWRVACLQNPHGILCCARGGQ
                     RSHIVQRWLHDAGIDYPLVEGGYKALRQTAIQATIELAQKPIVLIGGCTGSGKTLLVQ
                     QQPNGVDLEGLARHRGSAFGRTLQPQLSQASFENLLAAKMLKTDAHQDLRLWVLEDES
                     RMIGSNHLPECLRERMTQATIAVVEDPFEIRLERLNEEYFLRMHHDFTHAYGDEQGWQ
                     EYCEYLHHGLSAIKRRLGLQRYNELAARLDAALTTQLTTGSTDGHLAWLVPLLEEYYD
                     PMYRYQLEKKAEKVVFRGEWAEVAEWVKAQ"
     gene            complement(10402..11328)
                     /gene="allS"
                     /locus_tag="J1Y83_003079"
     CDS             complement(10402..11328)
                     /gene="allS"
                     /locus_tag="J1Y83_003079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator AllS"
                     /protein_id="HBA7631206.1"
                     /translation="MFDPETLRTFIAVAETGSFSKAAERLCKTTATISYRIKLLEENT
                     GVALFFRTTRSVTLTAAGEHLLCQARDWLSWLESMPSELQQVNDGVERQVNIVINNLL
                     YNPQAVARLLAWLNERYPFTQFHISRQIYMGVWDSLLYEGFSLAIGVTGTEALANTFS
                     LDPLGSVQWRFVMAADHPLANVEEPLTEAQLRRFPAVNIEDSARTLTKRVAWRLPGQK
                     EIIVPDMETKIAAHLAGVGIGFLPKSLCQSMIDNQQLVSRVIPTMRPPSPLSLAWRKF
                     GSGKAVEDIVTLFTQRRPEISGFLEIFGNPRS"
     gene            11558..12040
                     /gene="allA"
                     /locus_tag="J1Y83_003080"
     CDS             11558..12040
                     /gene="allA"
                     /locus_tag="J1Y83_003080"
                     /EC_number="4.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ureidoglycolate lyase"
                     /protein_id="HBA7631207.1"
                     /translation="MKLQVLPLSQEAFSAYGDVIETQQRDFFHINNGLVERYHDLALV
                     EILEQDRTLISINRAQPANLPLTIHELERHPLGTQAFIPMKGEVFVVVVALGDDKPDL
                     STLRAFITNGEQGVNYHRNVWHHPLFAWQRVTDFLTIDRGGSDNCDVESIPEQELCFA
                     "
     gene            12118..12933
                     /gene="allR"
                     /locus_tag="J1Y83_003081"
     CDS             12118..12933
                     /gene="allR"
                     /locus_tag="J1Y83_003081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000141283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor AllR"
                     /protein_id="HBA7631208.1"
                     /translation="MTEVRRRGRPGQAEPVAQKGAQALERGIAILQYLEKSGGSSSVS
                     DISLNLDLPLSTTFRLLKVLQAADFVYQDSQLGWWHIGLGVFNVGAAYIHNRDALSVA
                     GPFMRRLMLLSGETVNVAIRNGNEAVLIGQLECKSMVRMCAPLGSRLPLHASGAGKAL
                     LYPLAEEELMSIILQTGLQQFTPTTLVDMPTLLKDLEQARELGYTVDKEEHVVGLNCI
                     ASAIYDDVGSVVAAISISGPSSRLTEDRFVSQGELVRDTARDISTALGLKAHL"
     gene            13023..14804
                     /gene="gcl"
                     /locus_tag="J1Y83_003082"
     CDS             13023..14804
                     /gene="gcl"
                     /locus_tag="J1Y83_003082"
                     /EC_number="4.1.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate carboligase"
                     /protein_id="HBA7631209.1"
                     /translation="MAKMRAVDAAMYVLEKEGITTAFGVPGAAINPFYSAMRKHGGIR
                     HILARHVEGASHMAEGYTRATAGNIGVCLGTSGPAGTDMITALYSASADSIPILCITG
                     QAPRARLHKEDFQAVDIEAIAKPVSKMAVTVREAALVPRVLQQAFHLMRSGRPGPVLV
                     DLPFDVQVAEIEFDPDMYEPLPVYKPAASRMQIEKAVEMLIQAERPVIVAGGGVINAD
                     AAALLQQFAELTSVPVIPTLMGWGCIPDDHELMAGMVGLQTAHRYGNATLLASDMVFG
                     IGNRFANRHTGSVEKYTEGRKIVHIDIEPTQIGRVLCPDLGIVSDAKAALTLLVEVAQ
                     EMQKAGRLPCRKEWVADCQQRKRTLLRKTHFDNVPVKPQRVYEEMNKAFGRDVCYVTT
                     IGLSQIAAAQMLHVFKDRHWINCGQAGPLGWTIPAALGVCAADPERNVVAISGDFDFQ
                     FLIEELAVGAQFNIPYIHVLVNNAYLGLIRQSQRAFDMDYCVQLAFENINSSEVNGYG
                     VDHVKVAEGLGCKAIRVFKPEDIAPAFEQAKVLMAQYRVPVVVEVILERVTNISMGSE
                     LDNVMEFEDIADNAADAPTETCFMHYE"
     gene            14817..15593
                     /gene="hyi"
                     /locus_tag="J1Y83_003083"
     CDS             14817..15593
                     /gene="hyi"
                     /locus_tag="J1Y83_003083"
                     /EC_number="5.3.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxypyruvate isomerase"
                     /protein_id="HBA7631210.1"
                     /translation="MLRFSANLSMLFGEYDFLARFEKAAQCGFRGVEFMFPYDYDIEE
                     LKQVLASNKLEHTLHNLPAGDWAAGERGIACIPGREEEFRDGVAAAIRYARALGNKKI
                     NCLVGKTPTGFSSEQIHTTLVENLRYAANMLMKEDILLLIEPINHFDIPGFHLTGTRQ
                     ALKLIDDVGCCNLKIQYDIYHMQRMEGELTNTMTQWADKIGHLQIADNPHRGEPGTGE
                     INYEYLFTVIENSDYNGWVGCEYKPQTTTEAGLRWMDPYR"
     gene            15693..16571
                     /gene="glxR"
                     /locus_tag="J1Y83_003084"
     CDS             15693..16571
                     /gene="glxR"
                     /locus_tag="J1Y83_003084"
                     /EC_number="1.1.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-3-oxopropionate reductase"
                     /protein_id="HBA7631211.1"
                     /translation="MKLGFIGLGIMGTPMAINLARAGHQLHVTTIGPVADELLSLGAV
                     SVETARQVTDASDIIFIMVPDTPQVEDVLFGENGCTKASLKGKTIVDMSSISPIETKR
                     FARQVNELGGDYLDAPVSGGEIGAREGTLSIMVGGDEAVFECVKPLFELLGKNITLVG
                     GNGDGQTCKVANQIIVALNIEAVSEALLFASKAGADPVRVRQALMGGFASSRILEVHG
                     ERMIKRTFNPGFKIALHQKDLNLALQSAKALALNLPNTATCQELFNTCAANGGSQLDH
                     SALVQALELMANHKLA"
BASE COUNT         3887 a         4181 c         4497 g         4082 t
ORIGIN      
        1 ctgtttctgg actaaacgtg aagcggttcc tgatgtgtgc taaaatgcgt ttctttgata
       61 aatctcaatt tttaagaggt ttacggcagg caggaaaatt taaatgaatg aatttgagaa
      121 gatttttaat gaaatgaatc tggaccgggc attgctaccg atcctttttc gttcgaaccg
      181 gtcgacagtg tggaagtatt taagcggcga tagtacggct cctgcctctg ccatgtcgct
      241 gattatgctg ttgcagctga tccagaaacg aaaccccgat ctgttagccg aatggttaac
      301 gctttctgat ttcaccattc cgccagaagt ttacctggac caacctgatt actggaaagg
      361 gtgggtgtat acccaacaca aggtcaataa aaacgtcctg gaatatctaa aaaaacacta
      421 tccggatgaa gaccaaaaaa gtatgggtaa aggccgggaa gagtaatttt gcctctactc
      481 ggctatatgc cggatgcggc gcaagcgcct tatctggcct acgattcggg catattttgt
      541 aggcatgata agacgcgcca gcgtcgcatc aggcgatgac cgcacggata atcagcgtta
      601 ttccttctat cgcaatcacc tcaacatgcg taccagcgcc gagatcctcg ctggcgctga
      661 caggccatga actgtcaccg acgcgcatat gaccacgccc gttgaccagc ggagattcca
      721 gaacaaaacg tcggccaatc aactgctgcc cgcgctggtt taaatgactg tcgctgtgct
      781 tttgttcgcg cacccgccgc gacaaccatt tccaccacag ccaggcggcg agcagcgtca
      841 ggacggcaaa cattacccct tgccactccc aacccagcgg cagcaaccag accaccagac
      901 cggtaatcac tgctgccacg ccgctccaca acagataacc attgccgccc aacatctcgg
      961 ctgccagcag caaaccgccg agacttagcc agaaaatatg tggatgaacg accattaact
     1021 ccatcatggc tgagtccgtt tgttggcgct gtctttcacc agctcagcaa tcccggcaat
     1081 cgaccccatc aggctgctgg cctctaatgg catcatcacc actttgctgt tactggacga
     1141 accgatctgc tgtaacgctt cggtgtattt ctgtgcgacg aagtagttca ccgcctgaat
     1201 atcaccggag gcgatggctt cagacaccat tttggtggcg cgggcttctg cttcagcgga
     1261 acgttcacgc gcttcagcct gtaaaaacgc cgactgacgt tcgccttccg ctttcaggat
     1321 ttgtgactgt ttttcacctt cggctttgag gatttccgcc tgacggatcc cttccgcttc
     1381 aaggatgtaa gcgcgtttgg tacgttccgc tttcatctgc gcgttcattg aagagataag
     1441 ctctgcgggc gggcgcacat cacgaatttc gatacgggtg actttaatcc cccacgggtt
     1501 ggtggcctca tcgacaatat gcagcagacg cgagttaatg ctgtcgcgct gagagagcat
     1561 ttcatcaagt tccattgaac ccagcacggt acggatgtta gtcatggtca ggttgatgat
     1621 cgccagctcc agattgctga cttcataagc cgcgcgcggc gcgtcaatca cctgaataaa
     1681 gcacacggcg tcgatggtaa cgttggcgtt atctttcgag ataacttcct gggaagggat
     1741 gtcgagcact tgctccatca tattgatctt gcgaccaatg cgatccataa acggcaccac
     1801 caggcttaga cctggctgta acgttttggt atagcgacca aagcgttcta ccgtccattg
     1861 atagccctgc ggcacgattt tgacgcccgc gccgacaatg accagcgcga caaaaatgag
     1921 aatcgggata aagataagca tcggaaaaac ctcctgttgt accgtccata atcagcaaaa
     1981 ttgctgcttg attaaacaaa ttatacctga ttactgaaag agagttcccc cttattcctg
     2041 cgaaggataa actgttttta gtaaaaatca gaaaaaggga acagcgatgc aggaaaatag
     2101 tcctttgctt cagctacaaa acgtaggata tctggcgggt gatacgaaga ttcttaataa
     2161 catcaatttt tcgctgtgtg ctggcgaatt taagttaatt accggtcctt ctggttgtgg
     2221 caaaagtaca ctgctaaaaa tagttgcttc attgatcagc ccaaccagcg gaacgttact
     2281 gtttgaaggt gaggatgtca gcacactaaa gccagaaatc taccgtcagc aagtctctta
     2341 ctgcgcccag acgccgacgc tgtttggcga tacggtatac gataacctga tttttccctg
     2401 gcagatccgt aaccagcagc ctgacccggc catttttctc gattttcttg aacgcttcgc
     2461 tttaccggac agcattttga cgaagaatat cgccgagcta tctggtggtg aaaaacaacg
     2521 catctcattg attcgtaacc tgcaatttat gccgaaggtt ttattgctcg acgaaatcac
     2581 cagtgcgctg gatgaaagta ataaacataa cgttaatgag atgatccacc gttatgtacg
     2641 cgagcaaaat attgctgtgc tgtgggtgac acacgataaa gacgaaatta atcatgcgga
     2701 taaagtgatt acactgcaac cgcatgctgg agaaatgcag gaagcacgct atgaactcgc
     2761 ataatattac taacgaatca ttagcactgg cattgatgct ggtggtggtg gcaatcttaa
     2821 ttagccataa agaaaaactg gcgctggaga aagatattct ctggagcgtc gggcgcgcga
     2881 taatccagct gattattgtc ggctatgtgc tgaagtatat tttcagcgtg gatgatgcct
     2941 gtctgacatt attgatggtg ttatttatct gctttaatgc ggcgtggaac gcgcaaaaac
     3001 gcagtaaata tattgctaaa gcttttatct catcgtttat tgccattacg gtcggagcgg
     3061 gaattaccct ggcggtactg atcctctccg ggtcgattga atttatcccg atgcaggtga
     3121 tccctatcgc cgggatgata gccggcaacg ccatggtagc ggtggggttg tgttacaaca
     3181 atttagggca aagggtcatt agcgaacagc aacagattca ggagaaactg agtcttggcg
     3241 cgacgccgaa acaggcttca gcgatattga ttcgcgacag tattcgcgcg gctttaattc
     3301 cgacggtcga ttcagcaaaa acggtaggct tagtgagttt accaggaatg atgtccgggc
     3361 tgatatttgc cgggattgat ccggtgaagg cgattaaata tcagattatg gtgaccttta
     3421 tgctgctctc aaccgccagc ctgtctacca ttattgcctg ctatttaacc tatcgtaagt
     3481 tttacaattc gcgccaccag ttggtggtga cgcaattgaa gaagaaatga tgcgatgccg
     3541 gacacgcagc atcgcatccg gcgttgtggt tcatgtgccg atcaacaaaa catcaataca
     3601 acaacgcata cagctggcgg cgatacttcg acgccagtgc atcacccgta cccagcgcag
     3661 cgaggatctc ctggaatgtt ttacgtgtct gaccatctgc ggcggtgaga tctttgcgca
     3721 gatgcccaaa cagtaactcc agcgcctctt cattgcgccc aacctggtgc agttgcagtg
     3781 ccagttgcgt tgccagtgcg gcatcttctg gattctctgc cacctgctgt tgcagctgtt
     3841 gaatttccgg cgtatctgcc gcctgcttca gcagttcgat ttgcgctacc agcccctggt
     3901 agcgggtgtc ctgatcctgt aacggaatgg ttttcagcac ggcttctgca tcttcagaac
     3961 ggttcagcgc aatcagcgtt tctgccagca gcaggccgat ttccccgttc tgattcgaca
     4021 actgccaggc gtctttcagc aatggcaggg cgtcggtgta attgccttcc tgcatcagct
     4081 gcatcgcctg ctgcgctttc agctcttctt cgcgcggcag caccttatcc agcagggagc
     4141 ggatcgcctc ttccggttgc ggcccctgga agccgtctac cggttgcccg ttctggaaca
     4201 gatacacggt cggaatcgcg cgcagaccaa actgggcggc aatcatctgc tccgcgtcgc
     4261 agtccagctt cgccagaata aattgcccgt tgtactgcgc cgcaaggttt tccagaattg
     4321 gggttaactg caaacagtgc tggctacgtt cagaccaaaa atagaacagc accggagtgg
     4381 tcatcgactg ttcaagaacc tgttgcaggt tagattcgtt aatgttgaca atattttcta
     4441 cggacatgga gtcgctctct gttgtcgata ttttctttga catgggggct taagcgcgcg
     4501 cttcaactca cccctgcaat attttgtcca tcacgcgccc cggcagcagg cgcttaagca
     4561 ccattactgc ccaggtcacc agcgttaccg gatagcgcat cttcggcttc tcgctaataa
     4621 aagcatggcg tactttgtcg accaccgctt ccggccccaa cgtaaagcgg gcggcgatgc
     4681 cgggattttc gaccggttta tcgctttgtg tctggttgac gttgtcggtg aagcgagtac
     4741 gaatgggacc gggttcgatc aggctaactt taattccgct gtggcgtagc tccatgcgca
     4801 gcgcatccga ccacgcctcc agcgcatatt tactggccgc gtaagcgcca cgacccggcg
     4861 tggagattaa tcccattacc gatgaggtca tcacaatacg cccttcaccg tgcggtaaca
     4921 tcgcgggtaa caggcgcatg gtgagctggt gtgcgccgaa aaagttggca gaaaactgct
     4981 gttccatctg cgcgcggctg atagtggaaa gggggccata catgccgaat ccggcattgt
     5041 taaagatccc atacagacaa ttatcggtca gggcgatcac ttcgtcggct gcgcgatcaa
     5101 cactttctgg caaatccaga tcgatcaaca cgccggtgaa tcccatattg ttcatgcgct
     5161 caacatcatc cggtttccgg caaccggcca gcacatgaaa accctggcgt tttaattcga
     5221 gcgcgctttc caggccaatt ccactggaac atccggtaat taagaccgat ttttgcataa
     5281 ctttacctgt caggatctcc gttgctttat gagtcatgat ttactaaaag ctgcaactgc
     5341 ttcgccatcc agtcggcaat aaacggctgg gcgtcgcggt tgggatgaat accgtcatcc
     5401 tgcatccatt gtggcttgag gtagacctct tccataaaaa agggcagcag cggaacatca
     5461 aactctttgg cgagtttggg gtaaatggcg ctaaaggctt cattataacg gcgaccatag
     5521 tttgcaggca gacgtatttg cattaacaat ggttcagcgt tggcggcttt gacatcctgc
     5581 aaaatctggc gcagcgtttg ctcggtttgt tgtggctgaa aaccacgcaa accgtcattg
     5641 ccgcccagtt caaccagcac ccagcgcggc tgatgctgtt tcagcagagc cggaaggcgc
     5701 gccagccctt gttgcgaggt gtcaccgctg atgctggcat tgactaccga cgttttactc
     5761 tgccacttat cattcaacaa ggcaggccag gccgcgctgg cagacattcg atacccggcg
     5821 ctcaggctat cacccagaat caataacgtg tctgctgcgg cggcacggaa ggttaacagg
     5881 accaggaaca ggaagggcaa atgccagcgg aaaacattgt tgaagttcat catcttaaga
     5941 agtccgtcgg tcagggggag catgaactct ccatcctcac cggagttgag ctggttgtca
     6001 aacgtggcga gaccatcgca ctggtgggcg agtcgggatc gggtaagtca accttgctgg
     6061 cgatcctcgc cgggctggat gacggcagca gtggcgaagt gagactggtg ggacaaccgc
     6121 tacataatat ggacgaagaa gcgcgggcaa agttgcgcgc gaagcacgta ggctttgttt
     6181 ttcagtcatt tatgttaatt cctaccctta acgcgctgga aaacgtcgag cttccggcgc
     6241 tgctgcgcgg tgagagtagc gcagaaagtc gtaacggggc gaaagcgttg ctcgaacagt
     6301 taggactggg taaacgtctg gatcatcttc cggcacagct ttccggcggc gaacagcaac
     6361 gagtggcgct ggcgcgagct tttaatggtc ggcctgatgt gctgtttgcc gacgaaccca
     6421 ccggcaatct tgaccgccag acgggcgata aaattgccga cctgctgttt tccctcaacc
     6481 gtgagcatgg caccacgttg attatggtga cccatgacct gcaactggcg gcacgctgtg
     6541 accgctgctt acggctggtg aacgggcagt tgcaggagga aacatgattg cacgttggtt
     6601 ctggcgcgaa tggcgctcgc cgtcgctatt aattgtctgg ctggcgttaa gcctggcggt
     6661 ggcctgcgtg ctggcgctgg gcaatatcag cgatcgcatg gagaaaggtt taagccagca
     6721 aagccgtgag tttatggcgg gcgatagggc gttgcgcagt tcgcgcgaag tgccgcaagc
     6781 gtggctggag gaagcgcaaa agcgcggcct gaaagtcggc aagcagctga ctttcgccac
     6841 catgaccttt gcaggcgaca caccgcagct ggcgaacgtc aaagcggtgg atgatatcta
     6901 cccgatgtat ggcgagctac aaaccaatcc ccctggcctg aaaccgcagg cgggcagcgt
     6961 attgctggcc ccacgcctga tggcgctcct taacctgaaa acgggcgaca ccatcgacgt
     7021 gggcgatgct accttgcgga ttgccggaga agtgattcag gaacctgatt ccggttttaa
     7081 ccccttccag atggctccgc gtctgatgat gaatctggcg gatgtcgata aaaccggagc
     7141 tgtgcagccg gggagtcggg tcacctggcg ctataaattc ggcggcagcg agaaccagct
     7201 cgacggctat gagaaatggc tgttacccca gcttaaaccc gaacaacgct ggtacggtct
     7261 ggaacaggac gaaggcgcgc tggggcgatc gatggaacgc tcgcaacagt tcctgctgct
     7321 ttcggcgctt ctgaccttgc tgctggcagt ggcagcggtg gcggtggcga tgaatcatta
     7381 ctgtcgcagt cgctacgatc tggtggcgat cctcaaaacg ctgggggcag ggcgagcgca
     7441 actgcgtaag ctaatcgtcg gtcagtggtt gatggtgctg gcgctttcag ccgttaccgg
     7501 cggggcgata ggcctgttgt tcgaaaacgt gttgatggtg ctgctcaagc ccgttctgcc
     7561 tgccgcacta ccgcctgcca gcctctggcc gtggctgtgg gcgctcggca ccatgacagt
     7621 catctcgctg ctggtgggcc tgcgaccgta ccgcttgttg ctggcaacgc agcctttacg
     7681 cgtattacgt aatgatgtgg tcgcgaacgt ctggccgctc aagttttatc tgccgattgt
     7741 cagtgtggtg gttgtgctgc tgcttgccgg gttaatgggc ggcagtatgc tgctttgggc
     7801 ggtgctggcg ggcgcggtag tactggcttt tctgtgcggt gttctgggct ggatgctgct
     7861 gaatgtactt cgccgcatga cgctgaaatc cctgcctctg cgcctggcgg ttagccgcct
     7921 gttacgtcag ccgtggtcaa cgttaagcca gctttcggca ttttcgctct cctttatgct
     7981 gctggcgctg ttgctggtgt tgcgtggcga tctgctcgac cgctggcaac agcagctacc
     8041 gccagaaagc ccgaactact ttttaatcaa catcgccaca gaacaggtta cgccgctaaa
     8101 aacgttcctc gcggaacatc aaatagtccc ggaatcgttt tatccggtgg tgcgggcgcg
     8161 actgacggcg attaacggca agccgacaga aggtaatgaa gatgaggcgc ttaaccgcga
     8221 actcaatctt acctggcaaa atacgcggcc cgatcataat ccgattgtcg ccggtaactg
     8281 gccaccaaaa gccgatgaag tgtcgatgga agaggggctg gcgaaacgct taaacgttgc
     8341 cctcggcgat accgtgactt ttatgggcga tacccaggag tttcgcgcca aagtaaccag
     8401 cctgcgcaaa gtggactggg aaagtctgcg gcctaatttc tattttattt tccctgaagg
     8461 ggcattagac gggcaaccgc agagctggct taccagtttc cgctgggaga atggcaacgg
     8521 catgttgacg caactcaacc gccagttccc gaccattagc ctgttagaca ttggcgcgat
     8581 tttaaaacag gtcggtcagg tgctggagca ggtaagtcgg gcgctggaag tgatggtggt
     8641 gctggtcacc gcctgcggca tgttgctgtt gctggcgcag gtgcaggtgg gaatgcgtca
     8701 gcgtcatcag gagctggtgg tgtggcgcac acttggtgcg gggaaaaaac tgctgcgcac
     8761 gacattgtgg tgtgagttcg ccatgctcgg gtttgtatcc ggcctggtgg ccgcaattgg
     8821 tgcggaaacg gcactggcgg tactgcaatc gaaagtgttt gatttcccgt gggagccaga
     8881 ctggcgattg tggattgttc tgccgtgcag cggtgcgcta ctgctgtcgc tttgcggcgg
     8941 ctggctgggt gcgcgactgc ttaagggtaa ggcgctgttc aggcagtttg cggggtgatg
     9001 acaggtgtta gattgcctga tgcgctacgc ttatcaggcc tacgaagatc gtgcaatata
     9061 ttgagttttc gaaattttgt aggccggata aggcgttcac gccgcatccg gcagttgtgc
     9121 gccgatgcca gatgcgacgc aggcgcgtct tatcatgcct acgaaacagc actcacctgt
     9181 aggctggata aggcgttcac gccgcatccg gcatgaacag cgtgcacaga atcccccatc
     9241 actgcgcctt aacccattcc gccacttccg cccactcacc gcgaaagaca actttttccg
     9301 cttttttctc aagctgatag cgatacatcg ggtcgtaata ttcttcaagt aacggcacca
     9361 gccaggccag atgaccgtcg gtgctgccgg tggtgagttg cgttgtcagc gccgcatcca
     9421 gccttgcagc cagttcgtta tagcgttgta gtcccagccg acgtttaatc gccgaaagcc
     9481 cgtgatgcag gtattcgcaa tactcctgcc agccctgttc gtcgccataa gcgtgggtaa
     9541 aatcatgatg catacgcaag aaatactctt cgttcaggcg ttcaagacgg atctcaaacg
     9601 gatcttctac caccgcaatc gtcgcctgag tcattcgttc gcgcagacat tccggcaggt
     9661 gattcgaacc gatcatccgg ctttcatctt ccagcaccca caggcgcaaa tcctgatggg
     9721 cgtcggtttt tagcattttg gcagccagca ggttttcaaa actcgcctgg ctaagttgtg
     9781 gttgtaacgt gcgaccaaaa gcagaaccgc gatggcgcgc cagcccttcc agatcaacac
     9841 cgttcggctg ttgttgtact aacagcgttt tgccgctgcc ggtacaaccg ccaatcagca
     9901 ctatcggttt ttgtgccagt tcaatggttg cctgaatcgc ggtctggcgc agtgccttat
     9961 aaccgccttc caccagcgga taatcaatcc ccgcgtcatg cagccagcgt tgcacaatat
    10021 gtgagcgctg accgccacgg gcgcagcaga gaataccgtg cggattttgc aggcacgcta
    10081 cccgccaggc gtccatgcgc tgctgacgaa tttcacccgc caccagttta tgccccagcg
    10141 ccagcgccgc gtctgaccct tgctgtttat agcagatgcc aacggcggcg cgttcatcgt
    10201 tattcattaa cggcaggttg atagcggcgg gcattgcgcc ttgctcaaac tcaatggggg
    10261 cgcgaacatc aataatgggc gtatcagcaa tcagcagggc acgatagtcc tgttccgtgt
    10321 gtctctcttg catagtaaaa agtgaacctc aaatcagctt gcgcgctatt ttacgcgcca
    10381 acgcgcaagg aaacttgatt tttaactgcg tgggttgccg aaaatttcta aaaatccgct
    10441 gatttccggc ctgcgctggg taaacaaggt cacaatatct tctaccgctt tgccgctgcc
    10501 aaatttgcgc catgccagac tcaatggcga aggagggcgc atcgttggga ttacccggct
    10561 gaccagttgt tgattatcga tcattgactg gcaaagtgat tttggcaaaa aaccaatgcc
    10621 aacgcccgcc agatgggcgg cgattttcgt ttccatatcg ggaacaataa tctctttttg
    10681 ccccggcaat cgccaggcga cgcgtttggt taaggtgcgg gcgctgtctt caatattgac
    10741 cgccggaaag cgccgcaact gtgcttctgt tagcggctct tcaacgttcg ccagcggatg
    10801 atccgccgcc atgacaaagc gccattgcac cgatcctaag ggatcaagac taaaggtatt
    10861 tgccagcgcc tcggttcccg tgacgccgat agccagtgaa aaaccttcgt acaatagcga
    10921 gtcccagacg cccatataga tttgtcggga gatgtgaaat tgggtaaacg ggtaacgttc
    10981 atttagccac gccagcagcc tggcaacggc ctgagggtta tagagcaggt tgttgatgac
    11041 gatattcacc tggcgttcca cgccatcatt cacctgttgt aactcgctgg gcatactttc
    11101 cagccagctc agccagtctc tggcctgaca aagtagatgc tcgccagccg ccgtcaacgt
    11161 cacgctgcga gtcgtgcgga aaaacagcgc cactccggta ttctcttcca gaagtttaat
    11221 gcgataactg atcgtcgccg tggttttaca taatcgttct gccgcttttg aaaaacttcc
    11281 tgtttcagca accgcaatga aagtccgcaa ggtttctgga tcgaacatct tcaggtatcc
    11341 ccttttaaat cagcaagttg cgtgattttc ttatcctctg atttatcagt atttttacat
    11401 gataaccctg ttcaatttgt ggactaaatc tagttttgga aaaatattcc aacttttgtg
    11461 ttgatgttgt tctcttaagg ttttagattg cctgttattg aaaccaagct gaccggtcgg
    11521 cgctggttga acggaattat gttacaagga caaaaagatg aaacttcagg tattaccgtt
    11581 aagtcaggaa gcctttagtg cttatggcga cgtaatcgaa acgcagcaac gggatttttt
    11641 ccatattaac aatggcctgg tggagcgcta tcacgatttg gcgctggttg agattcttga
    11701 gcaagaccgt acgcttatca gcattaaccg cgcgcaaccg gcgaatctgc cgctgaccat
    11761 tcacgaactc gaacgtcatc cgttgggcac tcaggccttt atcccgatga aaggtgaagt
    11821 gtttgtggta gtcgtggcgt taggtgacga caaaccagac ctgtcaaccc tgcgggcgtt
    11881 tattaccaac ggcgaacagg gagtgaatta ccatcgtaac gtctggcatc acccactttt
    11941 cgcctggcag cgcgtcaccg attttctgac catcgatcgc ggcggcagtg acaactgtga
    12001 tgttgaaagt attcctgaac aggaactctg ttttgcgtga cgcctgcaac cgacttgcat
    12061 aagataaacg aattgttcat tgtttatgct catttgtagg tcggagttaa cgtaggtatg
    12121 acggaagtta gacggcgcgg caggccagga caggcggagc ctgtagcaca gaagggcgca
    12181 caggcgttag agcggggaat tgcgattctg caatatctgg aaaaaagtgg gggaagttcg
    12241 tcggttagcg atatctctct caatctggat ttgccgctct ccacgacctt tcgcttgctg
    12301 aaggttttac aggcagcgga ttttgtctat caggacagtc agttaggctg gtggcatata
    12361 ggattaggtg tctttaacgt cggtgcagcg tacatccata accgcgatgc cctctccgtc
    12421 gccgggccgt ttatgcgccg cctgatgtta ctttccggcg aaacggtcaa tgtcgcgatc
    12481 cgtaacggca atgaagcggt attaattggt cagttagagt gtaaatcgat ggtcagaatg
    12541 tgtgcgccgc tgggcagtcg tctgccgctg catgcttccg gtgcgggcaa ggcgctgctt
    12601 tatccgctgg cggaagaaga gttgatgagc atcattctgc aaaccggttt gcagcagttt
    12661 acgccaacga cgcttgtgga tatgcccacc ttgctgaagg acctggaaca agcgcgtgaa
    12721 ctgggctata ccgtagataa agaagagcat gttgtcggcc tgaattgcat agcttcagca
    12781 atttacgatg atgtaggtag tgttgttgcc gctatctcca tctccgggcc ttcatcaaga
    12841 ctgacagaag atcgttttgt cagtcagggt gagctggtca gagacaccgc ccgcgatatc
    12901 agcacggcgt tgggactgaa agcacatcta tagtgtctgt cgtatcccgc tctgcggagc
    12961 gggttttttt gacaaaattt gaaagttgga aaatttttcc aattaataga ggtaggaata
    13021 aaatggcaaa aatgagagcc gttgacgcgg caatgtatgt gctggagaaa gaaggtatca
    13081 ccaccgcctt cggcgttccg ggagctgcaa tcaatccgtt ctactcagcg atgcgtaagc
    13141 acggcggtat tcgtcacatt ctggcgcgtc atgtggaagg tgcttcgcac atggcggaag
    13201 gttatacccg cgcaacggca gggaatatcg gcgtatgtct ggggacttcc ggtcctgcgg
    13261 gcacggacat gatcaccgcg ctctattccg catctgctga ttccattcct attctgtgta
    13321 ttaccggcca ggctccgcgc gcccgtctgc ataaagaaga tttccaggcc gttgatattg
    13381 aagcaattgc taaaccggtc agcaaaatgg cggttacagt tcgtgaagcg gcgctggtgc
    13441 ctcgtgtgct gcaacaggcg tttcacctga tgcgttctgg tcgcccgggt ccggtactgg
    13501 tggatttacc gttcgacgta caggttgcgg aaatcgagtt tgatcctgat atgtacgaac
    13561 cgctgccggt ctacaaacct gctgccagcc gtatgcagat cgaaaaagct gtagaaatgt
    13621 taatccaggc cgaacgtccg gtgattgttg ccggaggcgg ggtaatcaat gctgacgcag
    13681 ctgcactgtt acaacagttt gctgaactga ccagcgttcc ggtgatccca acgctgatgg
    13741 gctggggctg tatcccggac gatcatgaac tgatggccgg gatggtgggc ctgcaaaccg
    13801 cgcatcgtta cggtaatgca acgttgctgg cgtccgacat ggtgtttggt atcggtaacc
    13861 gttttgctaa ccgtcatacc ggttcggtag agaaatacac cgaagggcgc aaaatcgttc
    13921 atatcgatat tgagccgacg caaattggcc gcgtactgtg tccggatctt ggcattgtct
    13981 ctgatgctaa agcggcgctg acgctgctgg ttgaagtggc gcaggagatg caaaaagccg
    14041 gtcgtctgcc gtgtcgtaaa gagtgggtcg ccgactgcca gcagcgcaaa cgcactttgc
    14101 tgcgcaaaac ccacttcgac aacgtgccgg tgaaaccgca gcgcgtgtat gaagagatga
    14161 acaaagcctt tggccgcgat gtttgttatg tcaccaccat tggcctgtca caaatcgctg
    14221 cggcacaaat gctgcatgtc tttaaagacc gccactggat caactgtggg caggcaggcc
    14281 cgctgggctg gacgattccg gcggcgctgg gggtttgtgc cgctgatcca gagcgcaatg
    14341 tggtggcgat ttccggtgac ttcgacttcc agttcctgat tgaagaatta gccgttggcg
    14401 cacagttcaa cattccgtac atccatgtac tggtcaataa cgcctatctg ggtctgattc
    14461 gccagtcgca acgtgccttt gatatggatt actgcgtaca gctcgcgttc gaaaatatta
    14521 actccagcga agtgaacggt tatggcgtcg accacgtaaa agtagcggaa ggtttaggtt
    14581 gtaaagcaat tcgcgttttc aaaccggaag atattgcacc agcctttgaa caggcaaaag
    14641 tcttaatggc gcaatatcgg gtaccggtag tcgtggaagt tattctcgag cgtgtgacca
    14701 atatttcgat gggcagtgaa ctggataacg tcatggaatt tgaagatatc gccgataacg
    14761 cagcggatgc accgactgaa acctgtttca tgcactatga ataagggaga taaataatgt
    14821 tacgtttctc tgctaattta tcgatgttat ttggtgaata tgattttctc gcccgttttg
    14881 agaaagctgc gcagtgtggt tttcgcggcg ttgaatttat gttcccttat gactacgaca
    14941 ttgaagaatt aaaacaggtg ctggcgagta ataaactcga gcatacgctg cacaatttac
    15001 cagcgggtga ctgggcggcg ggcgagcggg gtattgcctg tattcctggc cgtgaagaag
    15061 agtttcggga tggcgtagca gcagcgattc gttatgctcg tgctctgggt aataaaaaaa
    15121 ttaactgtct ggtcggtaaa acgccgactg gtttcagcag tgaacagatt cacacaacgc
    15181 ttgtagaaaa cctgcgttat gctgcgaata tgctgatgaa agaagatatt ttattactga
    15241 ttgaacctat taaccatttt gatattcctg gtttccatct caccggaacc cggcaggcac
    15301 tgaaactgat tgacgatgtt ggttgctgca atttgaaaat tcagtatgac atttatcaca
    15361 tgcagcggat ggaaggtgaa ttaactaata ccatgactca gtgggccgat aaaattggtc
    15421 acctgcaaat tgctgataat ccgcatcgcg gcgaaccggg aaccggagaa attaattacg
    15481 aatatctctt tacggtaatt gaaaattctg actacaacgg ttgggttggc tgtgaatata
    15541 aaccccaaac caccacggaa gccggtttac gctggatgga tccgtaccgt taaaacgtaa
    15601 cgctattcag acaatgcttt tttaggccgc taagttggca ggggatcgtg ttgtctgaat
    15661 tcaggaaaag cgaaatttaa aagaggttaa ttatgaaact gggatttatt ggcttaggca
    15721 ttatgggtac accgatggcc attaatctgg cacgtgctgg tcatcaactg catgtcacaa
    15781 ccattggtcc ggttgctgat gaattactgt cactgggcgc cgttagtgtt gaaactgcgc
    15841 gccaggtaac agatgcatcg gacatcattt ttattatggt gccggatacg ccacaggttg
    15901 aagacgttct gtttggtgaa aatggttgta ccaaagcctc gctgaagggc aaaaccattg
    15961 ttgatatgag ctccatttcc ccgattgaaa ctaaacgttt cgctcgtcag gtgaatgaac
    16021 tgggcggcga ttatctcgat gcgccagtct ccggcggtga aatcggcgcg cgtgaaggga
    16081 cgttatcgat tatggttggc ggtgatgaag cagtatttga atgtgtaaaa ccgctgtttg
    16141 aactgctcgg taaaaatatc actctcgtgg gcggtaacgg cgacggtcaa acctgcaaag
    16201 tggcaaatca gattatcgtg gcgctcaata ttgaagcggt ttctgaagcc ttgttgtttg
    16261 cttcaaaagc cggtgcggac ccggtacgtg tgcgccaggc gctgatgggc ggctttgctt
    16321 cctcacgtat tctggaagtt cacggtgagc gcatgattaa acgcaccttt aatccaggtt
    16381 tcaaaattgc gctgcaccag aaagatctca acctggcact gcaaagtgcg aaagcactgg
    16441 cgctgaacct gccaaacacc gcgacctgcc aggaattgtt taatacctgt gcggcaaacg
    16501 gtggcagcca gttggatcac tctgcattag tgcaggcgct ggaattaatg gctaaccata
    16561 aactggcctg atacccgcaa taaaaatggc cgatatcaga aaatgaatcg gccagcaata
    16621 ttaaaaaaag aaagcagcca aagatgt
//
LOCUS       DADSYM010000108        16628 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2415
            SAMN08039579-rid11723173.denovo.108, whole genome shotgun sequence.
ACCESSION   DADSYM010000108 DADSYM010000000
VERSION     DADSYM010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039579
            Sequence Read Archive: SRR6362533
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16628)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16628)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980162.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 03:39:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,484
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 4,347
            CDSs (with protein)               :: 4,347
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 43 of 85
            Pseudo Genes (incomplete)         :: 37 of 85
            Pseudo Genes (internal stop)      :: 19 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16628
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039579-rid11723173.denovo.108"
                     /strain="LGE2415"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8302 N 95.9628 W"
                     /collection_date="2015-08-24"
     gene            complement(193..2625)
                     /locus_tag="J1Y83_003085"
     CDS             complement(193..2625)
                     /locus_tag="J1Y83_003085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000110794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aspartate kinase/homoserine
                     dehydrogenase II"
                     /protein_id="HBA7631212.1"
                     /translation="MSVIAQAGAKGRQLHKFGGSSLADVKCYLRVAGIMAEYSQPDDM
                     MVVSAAGSTTNQLINWLKLSQTDRLSAHQVQQTLRRYQCDLISGLLPAEEADSLISAF
                     ISDLERLAALLDSGINDAVYAEVVGHGEVWSARLMSAVLNQQGLPAAWLDAREFLRAE
                     RAAQPQVDEGLSYPLLQQLLVQHPGKRLVVTGFISRNNAGETVLLGRNGSDYSATQIG
                     ALAGVSRVTIWSDVAGVYSADPRKVKDACLLPLLRLDEASELARLAAPVLHARTLQPV
                     SGSEIDLQLRCSYTPDQGSTRIERVLASGTGARIVTSHDDVCLIEFQVPTSQDFKLAH
                     KEIDQILKRAQVRPLAVGVHNDRQLLQFCYTSEVADSALKILDEAGLPGELRLRQGLA
                     LVAMVGAGVTRNPLHCHRFWQQLKGQPVEFTWQSDDGISLVAVLRTGPTESLIQGLHQ
                     SVFRAEKRIGLVLFGKGNIGSRWLELFAREQSTLSARTGFEFVLAGVVDSRRSLLSYD
                     GLDASRALAFFNDEAVEQDEESLFLWMRAHPYDDLVVLDVTASQQLADQYLDFASHGF
                     HVISANKLAGASDSNKYRQIHDAFEKTGRHWLYNATVGAGLPINHTVRDLIDSGDTIL
                     SISGIFSGTLSWLFLQFDGSVPFTELVDQAWQQGLTEPDPRDDLSGKDVMRKLVILAR
                     EAGYNIEPDQVRVESLVPTHCEGGSIDHFFENGDELNEQMVQRLEAAREMGLVLRYVA
                     RFDANGKARVGVEAVREDHPLASLLPCDNVFAIESRWYRDNPLVIRGPGAGRDVTAGA
                     IQSDINRLAQLL"
     gene            complement(2628..3788)
                     /gene="metB"
                     /locus_tag="J1Y83_003086"
     CDS             complement(2628..3788)
                     /gene="metB"
                     /locus_tag="J1Y83_003086"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HBA7631213.1"
                     /translation="MTRKQATIAVRSGLNDDEQYGCVVPPIHLSSTYNFTGFNEPRAH
                     DYSRRGNPTRDVVQRALAELEGGAGAVLTNTGMSAIHLVTTVFLKPGDLLVAPHDCYG
                     GSYRLFDSLAKRGCYRVLFVDQGDEQALRVALAEKPKLVLVESPSNPLLRVVDIAKIC
                     HLAREVGAVSVVDNTFLSPALQNPLALGADLVLHSCTKYLNGHSDVVAGVVIAKDPDV
                     VTELAWWANNIGVTGGAFDSYLLLRGLRTLVPRMELAQRNAQAIVKYLQTQPLVKKLY
                     HPSLPENQGHEIAARQQKGFGAMLSFELDGDEHTLRRFLGGLSLFTLAESLGGVESLI
                     SHAATMTHAGMAPEARAAAGISETLLRISTGIEDGEDLIADLENGFRAANKG"
     gene            4065..4382
                     /gene="metJ"
                     /locus_tag="J1Y83_003087"
     CDS             4065..4382
                     /gene="metJ"
                     /locus_tag="J1Y83_003087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015855437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="met regulon transcriptional regulator MetJ"
                     /protein_id="HBA7631214.1"
                     /translation="MAEWSGEYISPYAEHGKKSEQVKKITVSIPLKVLKILTDERTRR
                     QVNNLRHATNSELLCEAFLHAFTGQPLPDDADLRKERSDEIPEAAKEIMREMGINPET
                     WEY"
     gene            4442..5050
                     /locus_tag="J1Y83_003088"
     CDS             4442..5050
                     /locus_tag="J1Y83_003088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiX family permuted papain-like enzyme"
                     /protein_id="HBA7631215.1"
                     /translation="MKIRLLILSLLVSVPAFAWQPQTGDIIFQISRSSQSKAIQLATH
                     SDYSHTGMLVIRNKKPYVFEAVGPVKYTPLKQWIAHGEKGKYVVRRVEGGLSVEQQQK
                     LAQTAKRYLGKPYDSSFSWSDDRQYCSEVVWKVYQNALGMRVGEQQKLKEFDLSNPLV
                     QAKLKERYGKNIPLEETVVSPQAVFDAPQLTTVAKEWPLFSW"
     gene            complement(5111..5323)
                     /gene="rpmE"
                     /locus_tag="J1Y83_003089"
     CDS             complement(5111..5323)
                     /gene="rpmE"
                     /locus_tag="J1Y83_003089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HBA7631216.1"
                     /translation="MKKDIHPKYEEITASCSCGNVMKIRSTVGHDLNLDVCSKCHPFF
                     TGKQRDVATGGRVDRFNKRFNIPGSK"
     gene            5526..7724
                     /gene="priA"
                     /locus_tag="J1Y83_003090"
     CDS             5526..7724
                     /gene="priA"
                     /locus_tag="J1Y83_003090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HBA7631217.1"
                     /translation="MPVAHVALPVPLPRTFDYLLPEGMTVKAGCRVRVPFGKQQERIG
                     VVVSVSDVSELPLNELKAVVEVLDVEPVFTHSVWRLLLWAADYYHHPIGDVLFHALPI
                     LLRQGRPAANAPMWYWFATEQGQAVDLNSLKRSPKQQQALAALRQGKIWRDQVATLEF
                     NDAALQALRKKGLCDLASETPEFSDWRTNYAVSGERLRLNTEQATAVGAIHSAADTFS
                     AWLLAGVTGSGKTEVYLSVLENVLAQGKQALVMVPEIGLTPQTIARFRERFNAPVEVL
                     HSSLNDSERLSAWLKAKNGEAAIVIGTRSALFTPFKNLGVIVIDEEHDSSYKQQEGWR
                     YHARDLAVYRAHSEQIPIILGSATPALETLCNVQQKKYRLLRLTRRAGNARPAIQHVL
                     DLKGQKVQAGLAPALITRMRQHLQADNQVILFLNRRGFAPVLLCHDCGWIAECPRCDH
                     YYTLHQAQQHLRCHHCDSQRPVPRQCPSCGSTHLVPVGLGTEQLEQTLAPLFPGVPIS
                     RIDRDTTSRKGALEQQLAEVHRGGARILIGTQMLAKGHHFPDVTLVALLDVDGALFSA
                     DFRSAERFAQLYTQVAGRAGRAGKQGEVVLQTHHPEHPLLQTLLYKGYDAFAEQALAE
                     RRMMHLPPWTSHVIVRAEDHNNQHAPLFLQQLRNLILSSPLADDKLWVLGPVPALAPK
                     RGGRWRWQILLQHPSRVRLQHIISGTLALINTIPDSRKVKWVLDVDPIEG"
     gene            7880..8905
                     /gene="cytR"
                     /locus_tag="J1Y83_003091"
     CDS             7880..8905
                     /gene="cytR"
                     /locus_tag="J1Y83_003091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CytR"
                     /protein_id="HBA7631218.1"
                     /translation="MKAKKQETAATMKDVALKAKVSTATVSRALMNPDKVSQATRNRV
                     EKAAREVGYLPQPMGRNVKRNESRTILVIVPDICDPFFSEIIRGIEVTAANHGYLVLI
                     GDCAHQNQQEKTFIDLIITKQIDGMLLLGSRLPFDASIEEQRNLPPMVMANEFAPELE
                     LPTVHIDNLTAAFDAVNYLYEQGHNRIGCIAGPEEMPLCHYRLQGYVQALRRCGIMVD
                     PQYIARGDFTFEAGSKAMQQLLDLPQPPTAVFCHSDVMALGALSQAKRQGLKVPEDLS
                     IIGFDNIDLTQFCDPPLTTIAQPRYEIGREAMLLLLDQMQGQHVGSGSRLMDCELIIR
                     GSTRALP"
     gene            8997..9956
                     /gene="ftsN"
                     /locus_tag="J1Y83_003092"
     CDS             8997..9956
                     /gene="ftsN"
                     /locus_tag="J1Y83_003092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsN"
                     /protein_id="HBA7631219.1"
                     /translation="MAQRDYVRRSQPAPSRRKKSTSRKKQRNLPAVSPAMVAIAAAVL
                     VTFIGGLYFITHHKKEESETLQSQKVTGNGLPPKPEERWRYIKELESRQPGVRAPTEP
                     SAGGEVKTPEQLTPEQRQLLEQMQADMRQQPTQLVEVPWNEQTPEQRQQTLQRQRQAQ
                     QLAEQQRLAQQSRTTEQSWQQQTRTSQAAPVQAQPRQSKPASTQQPYQDLLQTPAHTT
                     AQSKPQQAAPVARAADAPKPTAEKKDERRWMVQCGSFRGAEQAETVRAQLAFEGFDSK
                     ITTNNGWNRVVIGPVKGKENADSTLNRLKMAGHTNCIRLAAGG"
     gene            10049..10579
                     /gene="hslV"
                     /locus_tag="J1Y83_003093"
     CDS             10049..10579
                     /gene="hslV"
                     /locus_tag="J1Y83_003093"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008457544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease subunit HslV"
                     /protein_id="HBA7631220.1"
                     /translation="MTTIVSVRRNGHVVIAGDGQATLGNTVMKGNVKKVRRLYNDKVI
                     AGFAGGTADAFTLFELFERKLEMHQGHLVKAAVELAKDWRTDRMLRKLEALLAVADET
                     ASLIITGNGDVVQPENDLIAIGSGGPYAQAAARALLENTELSAREIAEKALDIAGDIC
                     IYTNHFHTIEELSYKA"
     gene            10589..11920
                     /gene="hslU"
                     /locus_tag="J1Y83_003094"
     CDS             10589..11920
                     /gene="hslU"
                     /locus_tag="J1Y83_003094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019841241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HslU--HslV peptidase ATPase subunit"
                     /protein_id="HBA7631221.1"
                     /translation="MSEMTPREIVSELDKHIIGQDNAKRSVAIALRNRWRRMQLNEEL
                     RHEVTPKNILMIGPTGVGKTEIARRLAKLANAPFIKVEATKFTEVGYVGKEVDSIIRD
                     LTDAAVKMVRVQAIEKNRYRAEELAEERILDVLIPPAKNNWGQTEQQQEPSAARQAFR
                     KKLREGQLDDKEIEIDLAAAPMGVEIMAPPGMEEMTSQLQSMFQNLGGQKQKARKLKI
                     KDAMKLLIEEEAAKLVNPEELKQDAIDAVEQHGIVFIDEIDKICKRGESSGPDVSREG
                     VQRDLLPLVEGCTVSTKHGMVKTDHILFIASGAFQIAKPSDLIPELQGRLPIRVELQA
                     LTTSDFERILTEPNASITVQYKALMATEGVNIEFTDSGIKRIAEAAWQVNESTENIGA
                     RRLHTVLERLMEEISYDASDLSGQTITIDADYVSKHLDALVADEDLSRFIL"
     gene            11987..12913
                     /gene="menA"
                     /locus_tag="J1Y83_003095"
     CDS             11987..12913
                     /gene="menA"
                     /locus_tag="J1Y83_003095"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HBA7631222.1"
                     /translation="MTEQQISRTQAWLESLRPKTLPLAFAAIIVGTALAWWQGHFDPL
                     VALLALITAGLLQILSNLANDYGDAVKGSDKPDRIGPLRGMQKGVITQQEMKRALIIT
                     VVLICLSGLALVAVACHTLADFVGFLILGGLSIIAAITYTVGNRPYGYIGLGDISVLV
                     FFGWLSVMGSWYLQAHTLIPALILPATACGLLATAVLNINNLRDINSDRENGKNTLVV
                     RLGAVNARRYHACLLMGSLVCLALFNLFSLHSLWGWLFLLAAPLLVKQARYVMREMDP
                     VAMRPMLERTVKGALLTNLLFVLGIFLSQWAA"
     gene            13006..13491
                     /gene="rraA"
                     /locus_tag="J1Y83_003096"
     CDS             13006..13491
                     /gene="rraA"
                     /locus_tag="J1Y83_003096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001392924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E activity regulator RraA"
                     /protein_id="HBA7631223.1"
                     /translation="MKYDTSELCDIYQEDVNVVEPLFSNFGGRASFGGQIITVKCFED
                     NGLLYDLLEQNGRGRVLVVDGGGSVRRALVDAELARLAVQNEWEGLVIYGAVRQVDDL
                     EELDIGIQAMAAIPVGAAGEGIGESDVRVNFGGVTFFSGDHLYADNTGIILSEDPLDI
                     E"
     gene            complement(13576..13821)
                     /gene="zapB"
                     /locus_tag="J1Y83_003097"
     CDS             complement(13576..13821)
                     /gene="zapB"
                     /locus_tag="J1Y83_003097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring assembly protein ZapB"
                     /protein_id="HBA7631224.1"
                     /translation="MTMSLEVFEKLEAKVQQAIDTITLLQMEIEELKEKNNSLSQEVQ
                     NAQHQREELERENNHLKEQQNGWQERLQALLGRMEEV"
     gene            14246..15091
                     /gene="glpF"
                     /locus_tag="J1Y83_003098"
     CDS             14246..15091
                     /gene="glpF"
                     /locus_tag="J1Y83_003098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol uptake facilitator protein GlpF"
                     /protein_id="HBA7631225.1"
                     /translation="MSQTSTLKGQCIAEFLGTGLLIFFGVGCVAALKVAGASFGQWEI
                     SVIWGLGVAMAIYLTAGVSGAHLNPAVTIALWLFACFDKRKVIPFIVSQVAGAFCAAA
                     LVYGLYYNLFFDFEQTHHIVRGSVESVDLAGTFSTYPNPHINFVQAFAVEMVITAILM
                     GLILALTDDGNGVPRGPLAPLLIGLLIAVIGASMGPLTGFAMNPARDFGPKVFAWLAG
                     WGNVAFTGGRDIPYFLVPLFGPIVGAIVGAFAYRKLIGRHLPCDICVVEEKETTTPSE
                     QKASL"
     gene            15114..16622
                     /gene="glpK"
                     /locus_tag="J1Y83_003099"
     CDS             15114..16622
                     /gene="glpK"
                     /locus_tag="J1Y83_003099"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HBA7631226.1"
                     /translation="MTEKKYIVALDQGTTSSRAVVMDHDANIISVSQREFEQIYPKPG
                     WVEHDPMEIWATQSSTLVEVLAKADISSDQIAAIGITNQRETTIVWEKETGKPIYNAI
                     VWQCRRTAEICEHLKRDGLEDYIRSNTGLVIDPYFSGTKVKWILDHVEGSRERARRGE
                     LLFGTVDTWLIWKMTQGRVHVTDYTNASRTMLFNIHTLDWDDKMLEVLDIPREMLPEV
                     RRSSEVYGQTNIGGKGGTRIPISGIAGDQQAALFGQLCVKEGMAKNTYGTGCFMLMNT
                     GEKAVKSENGLLTTIACGPTGEVNYALEGAVFMAGASIQWLRDEMKLINDAYDSEYFA
                     TKVQNTNGVYVVPAFTGLGAPYWDPYARGAIFGLTRGVNANHIIRATLESIAYQTRDV
                     LEAMQADSGIRLHALRVDGGAVANNFLMQFQSDILGTRVERPEVREVTALGAAYLAGL
                     AVGFWQNLDELQEKAVIEREFRPGIETTERNYRYAGWKKAVKRAMAWEEHDE"
BASE COUNT         3944 a         4433 c         4451 g         3800 t
ORIGIN      
        1 tacttaatgt cattttgtaa ttgtcattaa taacttcacc attcacactg tgaattcatc
       61 gctcaacaca aaagccccct tattaccggg gcactgacca aaggcaaaat gacaatcaat
      121 caccacctcc gcaggaggtg gtttagaggt gacaacaaaa gctggcaact gccagctttt
      181 gttcaataat cactacaaca actgcgccag acggttaata tccgactgaa tcgccccggc
      241 ggtgacgtcg cgccctgcac ctggcccgcg gatcaccagc gggttgtcgc gataccagcg
      301 gctttcgatg gcaaagacgt tatcgcatgg cagtagtgat gccagcggat ggtcttcacg
      361 caccgcttcc acgcccactc gcgctttgcc gttggcatcg aaacgcgcca cgtagcgcaa
      421 caccagcccc atttcacggg ccgcttccag tcgttgtacc atctgctcat taagttcatc
      481 gccattttca aagaaatggt cgatgcttcc accttcgcaa tgggttggca ccagtgattc
      541 aacacgcacc tggtccggtt caatgttgta acccgcttca cgcgccagaa tcaccagctt
      601 gcgcatcacg tctttgccgg agaggtcatc acgcgggtca ggttcagtta agccctgctg
      661 ccacgcctga tccaccagct cggtaaacgg cacgctaccg tcgaattgca ggaacagcca
      721 ggagagtgtg ccggagaaga tcccgctgat cgacaaaata gtatcgccgc tgtcgatcag
      781 atcgcgcacg gtgtggttga tcggcaagcc tgcgccgacg gtggcgttgt acagccagtg
      841 acgcccggtt ttttcgaagg cgtcgtggat ctggcgatat ttattgctgt cgctcgctcc
      901 tgccagtttg ttggcactga tgacgtggaa accgtggctg gcgaaatcaa gatactggtc
      961 ggcaagctgc tggctggcgg taacgtccag caccactaaa tcatcatacg gatgggcgcg
     1021 catccacagg aacagcgact cttcatcctg ctcgaccgct tcatcgttga agaaggctaa
     1081 cgcgcggctg gcgtccagcc cgtcatagct caacaggctg cggcggctgt ccaccacgcc
     1141 tgccagcaca aattcaaagc cggtacgcgc cgaaagcgtg ctctgctcac gggcgaacag
     1201 ttccagccag cgggaaccga tattgccctt gccgaacagt accaggccga tgcgtttttc
     1261 tgcgcggaag acggactgat gcagcccctg aatcaggctt tcggtcggcc cggtgcgcag
     1321 tactgccacc aggctgatgc cgtcatcgga ctgccaggta aattcgaccg gctggccttt
     1381 cagttgctgc cagaagcggt ggcaatgcag cgggttacgg gttacgcccg ccccaaccat
     1441 cgccaccagc gccagcccct gacgcaggcg cagttcgcca ggtaatcctg cttcgtcgag
     1501 gattttcagc gcactatcgg ccacttccga ggtgtagcaa aattgcagca actggcgatc
     1561 gttatgcacg ccaactgcca gtggacgcac ctgcgcgcgt ttcaggatct ggtcgatctc
     1621 tttatgcgcc agtttgaaat cctggctggt gggcacctga aactcaatca agcagacatc
     1681 atcgtggctt gtgacaatac gcgcaccagt accggaggcc agcacgcgtt caatgcgcgt
     1741 ggaaccttga tccggcgtgt agctacagcg cagctgcagg tcgatttcgc tgccagaaac
     1801 tggctgtaaa gtacgggcgt gaagaacagg agccgccaga cgcgccagtt cgctggcctc
     1861 atccagacgc agcaatggca gcagacaggc atctttcact ttacgcgggt cggcactgta
     1921 taccccggcg acgtcgctcc agatggttac acgagaaaca cccgccagcg caccgatttg
     1981 tgtcgcggaa tagtcggaac cgttacgccc aagcagcacc gtttcaccgg cgttgttacg
     2041 gctgataaac ccggtcacca ccagacgttt gcctggatgt tgtaccagta gctgttgcag
     2101 caacgggtaa gaaagccctt catcaacctg cggttgcgca gcgcgttcag cgcgtaaaaa
     2161 ctcgcgagca tcaagccagg ccgctggcag cccttgttga ttaagtaccg cagacatcag
     2221 gcgcgccgac cacacttcgc cgtggcccac cacttccgca tacaccgcgt cgttaatacc
     2281 gctgtcgagc agcgccgcca ggcgctcaag atcgctgata aaggcactaa tgaggctatc
     2341 ggcttcttcg gcgggtaaca ggccgctaat cagatcgcac tgatagcgac gcagcgtttg
     2401 ttgaacctga tgcgcagaga gacgatcggt ctggcttagt ttcaaccagt taatcaactg
     2461 gttagtggtg ctaccggcgg cggaaaccac catcatatcg tcaggctgag agtactccgc
     2521 cataatgccc gcgacacgca aataacactt cacatcagcc agactactgc caccaaattt
     2581 atgcagctga cgacctttcg cccctgcctg cgcaatcaca ctcattttta ccccttgttt
     2641 gcagcccgga agccattttc caggtcggca attaaatctt cgccatcttc aataccggtg
     2701 gagatacgca gcagcgtctc tgagatcccg gcggcagcac gcgcttctgg tgccatgcct
     2761 gcatgtgtca tggttgcggc gtgagagatt aaactttcca ctccccctaa tgattccgcc
     2821 agcgtaaaca acgacagccc gcctaggaaa cgacgcagcg tgtgctcatc gccatccagt
     2881 tcaaaactca acattgcgcc aaagcctttt tgctggcgcg cggcaatttc atgcccctga
     2941 ttttccggca gcgacgggtg atacagtttt ttcaccaacg gctgggtttg cagatatttc
     3001 acaatcgcct gcgcgttgcg ctgggccagc tccatacgcg gcaccagcgt tcgcaacccg
     3061 cgcagcagca gatagctgtc aaacgcgccg cccgtcacgc caatattgtt tgcccaccag
     3121 gcaagttcag tgacaacgtc cgggtcttta gcaatcacta cgccggctac cacgtctgag
     3181 tgaccgttca gatatttcgt acatgaatgc aacaccagat cggcacctaa tgccagcgga
     3241 ttttgtaatg ccgggcttaa gaaggtgtta tccaccacgc tcaccgcccc gacttccctt
     3301 gccagatggc agattttcgc aatatccacg acgcgtaaca atggattact tgggctttct
     3361 accagtacca gtttgggttt ttctgccagc gctacccgta atgcctgttc atcgccttga
     3421 tcaacaaaca acacgcgata gcaaccgcgt ttcgccagac tgtcgaacag gcgatagctg
     3481 ccgccatagc agtcgtgcgg cgcaaccagc agatcgcccg gtttcaaaaa gacggtcgtt
     3541 accaggtgaa tcgcggacat gccggtatta gtaagtacgg caccagcacc gccttccagt
     3601 tctgccagcg cacgctgaac cacatcgcgc gttgggttgc cgcgccgcga gtaatcatgc
     3661 gcgcgcggtt cattaaatcc ggtaaagtta taggtgctgg aaagatggat gggagggaca
     3721 acgcaaccat actgttcgtc gtcatttaac ccgctacgca ctgcgatggt ggcctgttta
     3781 cgcgtcatgt gatgaagttc cctgggcttg gtcggtgaaa tgtcaggcac cagagtaaac
     3841 attgtgttaa tggacgtcaa tacatctgga catctaaact tctttgcgta tagattgagc
     3901 aaatcccaaa tagccgttaa aattatatgc attatcatgc cgacaggtgc attacacgat
     3961 gtcacggtag cgcctgtacg gtaaactatg cgggtttacg gtcagtaccc acataaattg
     4021 tgtggtctgg tctcaattta ttgacgaaga ggattaagta tctcatggct gaatggagcg
     4081 gcgaatatat cagcccatac gctgagcacg gcaagaagag tgaacaagtc aaaaaaatta
     4141 cggtttccat tcctcttaag gtgttaaaaa tcctcaccga tgaacgcacg cgtcgtcagg
     4201 tgaacaacct gcgtcacgcg accaacagcg agctgctgtg cgaagcgttt ctgcatgcct
     4261 ttaccgggca acctttgccg gatgatgccg acctgcgtaa agagcgcagc gacgaaattc
     4321 cggaggcggc aaaagagatc atgcgtgaga tggggattaa cccggagacg tgggaatact
     4381 aaagcgaaaa aaagcggcgc ggagttaacc agccgctttt ttcatacatg gacgtttaac
     4441 gatgaaaatc aggctactga tcctcagcct gctggtttct gtgcctgcct ttgcctggca
     4501 accacaaacc ggcgacatca tctttcagat ctctcgctca tcgcaaagta aagcgatcca
     4561 actggcgacc cattccgatt acagccacac cggtatgctg gtgatacgca acaaaaagcc
     4621 ctacgttttt gaagcggtcg gcccggtaaa atacacccca ctcaagcagt ggatcgccca
     4681 tggtgaaaag ggcaaatacg ttgttcgccg cgttgaaggc ggactgagtg ttgaacaaca
     4741 gcaaaaactg gcgcaaacgg caaaacgtta tctcggtaaa ccgtatgact ccagcttctc
     4801 gtggagcgac gatcgccagt actgttcgga agtggtgtgg aaggtctacc agaatgcgct
     4861 gggaatgcgt gtgggcgagc agcagaagct gaaagagttc gatcttagta acccgctagt
     4921 tcaggcaaag ctcaaagaac gctacggcaa aaatattccg cttgaggaaa cggtagtttc
     4981 accacaagcc gttttcgacg cgccacaact caccacggtc gccaaagaat ggccgctgtt
     5041 ttcctggtaa tacgaccaaa attcaggcac aaaaaaagcg ccgtgcggcg cttttttcgg
     5101 aaatccggtc ttatttgctg cccgggatgt tgaaacgctt gttgaagcgg tcaacacggc
     5161 caccggtagc aacatcacgc tgtttgccag tgaagaacgg gtggcacttg ctgcacacgt
     5221 cgaggttcag gtcatgacca acggtggagc ggattttcat tacgttaccg caagagcagc
     5281 tagcagtaat ttcttcgtat ttcgggtgaa tatctttttt catgggaaaa cctcggttta
     5341 aggccgcgtc gctcttccag ccctaacgcc agacaccacg cgatgttaaa agtatagctt
     5401 caatacgatc atttcgtacg aaggcgcgaa atcatacaga aattaaccag cgtatgcaaa
     5461 ctgatccgca ctcttctacg gcaatgtgta tactaaccca ccgaatttca agtcaggatg
     5521 atgctatgcc cgttgcccac gttgccttgc ccgttccgct tcctcgtacc tttgactatc
     5581 tgctgccaga aggcatgacg gttaaagccg ggtgtcgcgt gcgcgtgccg tttggcaaac
     5641 agcaggagcg catcggggtt gtggtgtcag ttagcgatgt cagcgagctg ccgcttaacg
     5701 agctgaaagc ggtagttgaa gtgctggacg ttgagccggt gtttactcac tccgtctggc
     5761 gattactgct ctgggcggca gattactatc atcatccgat tggcgatgtg ctgtttcatg
     5821 ccttgccgat tttactacgc caggggcggc ctgcggcgaa cgcgccgatg tggtactggt
     5881 ttgccactga acaaggccag gcggtggatc tgaacagcct gaaacgctcg ccaaagcaac
     5941 aacaggcact ggcggcattg cggcaaggca aaatctggcg cgaccaagtt gccacgcttg
     6001 aatttaatga tgccgcgtta caggcgctgc gcaaaaaagg tctgtgtgat ttagcaagtg
     6061 aaacaccaga gtttagcgac tggcgaacga actatgccgt ttctggtgag cggttgcgat
     6121 tgaataccga acaggccacc gccgttggcg caattcatag cgcggcagat actttttctg
     6181 cctggctgct ggcgggcgtt accggttccg gtaaaacgga agtttatctc agcgtactgg
     6241 aaaacgtgct cgctcagggc aaacaggcgc tggtgatggt gccggaaatc ggcctgacac
     6301 cgcaaactat cgcccgtttt cgtgaacgtt ttaatgcccc cgtagaagtt ctgcattcca
     6361 gcctgaacga cagcgagcgt ctttcggcgt ggttgaaagc gaaaaatggt gaggcggcga
     6421 ttgtgatcgg cacccgctcc gcgctgttta cgccgtttaa aaatctcggc gtgattgtca
     6481 ttgatgaaga gcacgacagt tcctacaagc agcaggaagg ctggcgctat catgcccgcg
     6541 acctggcggt ttatcgtgcg cacagcgagc aaatcccgat tattcttggc tccgcaacac
     6601 ccgcgctgga aacgttatgc aacgtccagc agaaaaaata ccgcctgctg cgcctgacgc
     6661 gtcgggcggg gaatgcgcgt ccggcaattc aacacgtgct ggatttaaaa ggtcagaagg
     6721 tgcaggcggg tctggctccg gcgttaatca cccgtatgcg ccagcattta caggccgaca
     6781 accaggttat cctctttctt aaccgccgtg gctttgcgcc tgtcttgctg tgccacgact
     6841 gcggctggat tgccgaatgc ccacgttgtg atcactacta cacgctgcat caggcgcaac
     6901 agcatttgcg ctgtcaccac tgtgacagcc aacgcccggt gccgcgccag tgcccttcct
     6961 gcggttccac gcacctggtg ccggtggggc tgggcaccga acagcttgaa cagacgcttg
     7021 cgccgctgtt ccctggtgtg cccatttctc gtatcgaccg cgataccacc agccgcaaag
     7081 gggcgctgga acaacaactg gcggaagtac atcgcggcgg tgcgaggatt ttgatcggca
     7141 cgcaaatgct ggcgaaaggt caccactttc cggatgtgac gctggtggca ttactggacg
     7201 tagacggcgc gctgttttct gccgattttc gctcggcaga gcgtttcgcc cagctttaca
     7261 cccaagtcgc cggtcgtgcc gggcgtgcgg gtaaacaggg tgaagtggtg ctgcaaacgc
     7321 accatccgga acatcctctg ttgcaaacat tgctgtataa aggctatgac gcctttgcag
     7381 aacaggcgct tgccgagcgg cgaatgatgc atttgccgcc gtggaccagt catgtgattg
     7441 tgcgcgccga agatcataac aatcaacatg caccactgtt cctgcaacag ttgcgaaatc
     7501 tcatcctctc cagcccactg gcggatgaca agctctgggt actcggcccg gttccggcac
     7561 tggcccctaa gcgtggtggc cgctggcgct ggcagatatt gttgcagcac ccttcccgcg
     7621 tgcgcttgca acacatcatt agtggtacgc tggcgctcat caatacaata ccggattccc
     7681 gtaaggtgaa atgggtgctg gatgtcgatc ccatcgaggg ctaatgcgca aacgatgcga
     7741 ggcggatcga aaaattcaat attcatcaca cttttcatga aaattctgta accgttttca
     7801 cgcgctatct gctaaaaatg ttgccgatgt gaagtaaaca tggaagtagt acgcctgccg
     7861 tgccaggcga ggagtgagtg tgaaagcgaa gaagcaggaa actgccgcga ccatgaaaga
     7921 cgttgccctc aaggcaaaag tctctacagc gaccgtctcc cgagcattaa tgaatcccga
     7981 taaagtctcc caggccaccc gtaatcgggt tgaaaaagcg gcccgggaag tgggttattt
     8041 accgcagcct atgggacgca acgtcaagcg taatgaatcc cgtaccattc tggtgattgt
     8101 cccggatatc tgcgatccct tctttagcga aattattcgc ggtatcgaag ttacggcggc
     8161 aaatcacgga tatctggtgc tgattggcga ctgtgcgcat caaaatcagc aggaaaaaac
     8221 ctttatcgat ttgatcatca ccaagcaaat tgatggcatg ttgttgctgg gttcaaggct
     8281 gccgtttgat gccagcatcg aggaacagcg taatctgccg ccgatggtga tggcgaacga
     8341 atttgcaccg gaactggagc tgcctaccgt tcatatcgac aatctgaccg ccgcatttga
     8401 tgcagtaaat tatttatatg agcaagggca taatcggatt ggctgtatag ccggtcccga
     8461 agagatgccg ctgtgtcact accgcctgca aggctatgtc caggcgctgc gtcgctgcgg
     8521 cattatggtt gatccgcaat acatcgcccg tggcgacttc accttcgaag ccggaagcaa
     8581 agcaatgcaa cagctgcttg atcttccaca accgcccact gctgtcttct gccatagtga
     8641 tgtgatggcg ctcggcgcac tttcgcaggc aaaacgccag gggctaaaag tcccggaaga
     8701 cctttccata atcggttttg ataacatcga cctgacgcaa ttttgtgatc cgccgctgac
     8761 aaccatcgcg cagccgcgtt acgaaatcgg tcgggaagct atgctgttat tgcttgatca
     8821 aatgcagggg caacacgttg gcagtggctc tcgtttaatg gactgcgaac ttatcatccg
     8881 gggatcaaca cgcgcgttac cttaaagtaa acctttaaga ctgccgtgac tggtcaaagg
     8941 cccgccgctt aagtaacatg gcgggctgac gaacgaataa atacagcgaa acgatagtgg
     9001 cacaacgaga ttatgtacgc cgcagccaac cggcaccttc gcggcgaaaa aagagcacct
     9061 cacggaaaaa gcaacgaaat ctgcctgcgg tttctcccgc tatggtcgct attgctgccg
     9121 ccgttcttgt gacctttatc ggtggtctgt acttcattac gcatcacaag aaagaagagt
     9181 ccgagacgct gcaaagccag aaagtgaccg gaaacggact accaccaaaa ccagaagaac
     9241 gctggcgcta cattaaagag ctggaaagtc gccagccggg tgtacgtgcg cccacagaac
     9301 cttctgccgg tggtgaagtg aaaacgccag agcaactgac accagaacaa cgccagcttc
     9361 ttgagcaaat gcaggctgat atgcgccagc agccaacgca gctggttgaa gttccgtgga
     9421 atgaacagac gccggaacag cgtcagcaaa cgctacagcg ccagcgtcag gcgcagcaat
     9481 tagcggagca gcaacgcctg gctcagcagt cgcgtacgac tgaacaaagc tggcagcagc
     9541 agacgcgtac gtcgcaagcc gcgccggtac aggcgcaacc gcgccagtct aaaccggctt
     9601 ctactcagca gccgtaccag gatctgctgc aaacccctgc gcacacgacc gcgcagtcga
     9661 agccgcagca agctgcgccg gttgctcgtg ccgctgacgc gccaaaaccg acggcggaga
     9721 aaaaagacga acgccgctgg atggtgcagt gcggttcgtt cagaggcgcg gaacaggcag
     9781 agacggtacg tgctcagctg gcgttcgaag gctttgactc aaaaattacc accaacaatg
     9841 gctggaatcg tgtggtcatt ggcccggtga aaggcaaaga gaacgcagac agcaccctca
     9901 atcgcttgaa gatggcgggt catacaaact gcattcggct cgccgccggg ggttgaaacc
     9961 ctcaaaatcc cccccatcta taattgcatt atgccccgta cttttgtacg gggtttgtac
    10021 tctgtattcg taaccaaggg gtcagctcgt gacaactata gtaagcgtac gccgtaacgg
    10081 ccatgtggtc atcgctggtg atggtcaggc cacgttgggc aataccgtaa tgaaaggcaa
    10141 cgtgaaaaag gtccgccgtc tgtacaacga caaagtcatc gcgggctttg cgggcggtac
    10201 tgcggatgct tttacgctgt tcgaactgtt tgaacgtaaa ctggaaatgc atcagggcca
    10261 tctggtcaaa gccgccgttg agctggcgaa agactggcgt accgatcgca tgctgcgcaa
    10321 acttgaagca ctgctggcag tcgcggatga aactgcatcg cttatcatca ccggtaacgg
    10381 tgacgtggtg cagccagaaa acgatcttat tgctatcggc tccggcggcc cttacgccca
    10441 ggctgcggcg cgcgcgctgt tagaaaacac tgaacttagc gcccgtgaaa ttgctgaaaa
    10501 ggcgttggat attgcaggcg acatttgcat ctataccaac catttccaca ccatcgaaga
    10561 attaagctac aaagcgtaag gatctcccat gtctgaaatg accccacgcg aaatcgtcag
    10621 cgaactggat aagcacatca tcggccagga caacgccaag cgttccgtgg cgattgctct
    10681 gcgtaaccgc tggcgtcgca tgcagctcaa cgaagagctg cgccatgaag tgaccccgaa
    10741 aaatatcctg atgatcggcc cgaccggtgt cggtaaaact gaaatcgccc gtcgcctggc
    10801 taagctggcg aatgcgccgt tcatcaaagt tgaagcgacc aaattcaccg aagtgggcta
    10861 tgtcggtaag gaagtggatt ctattattcg cgatctgacc gatgccgccg tgaaaatggt
    10921 acgcgtccag gctatcgaga aaaaccgtta tcgcgctgaa gaactggcag aagaacgtat
    10981 tctcgacgta ctgatcccac cggctaaaaa taactgggga cagaccgaac agcagcagga
    11041 accgtccgct gctcgtcagg cattccgcaa aaaactgcgt gaaggccagc ttgatgacaa
    11101 agaaatcgag atcgatcttg ccgcagcacc gatgggcgtt gaaattatgg ctcctccggg
    11161 catggaagag atgaccagcc agctgcagtc catgttccag aacctgggcg gtcagaaaca
    11221 aaaagcgcgt aagctgaaaa tcaaagacgc catgaagctg ctgattgaag aagaagcggc
    11281 gaaactggtg aacccggaag agctgaagca agatgctatc gacgctgttg agcagcacgg
    11341 gatcgtgttt atcgacgaaa tcgacaaaat ctgtaagcgc ggcgagtctt ccggtccgga
    11401 tgtttctcgt gaaggcgttc agcgtgacct gctgccgctg gtagaaggtt gcactgtttc
    11461 caccaaacat gggatggtca aaactgacca cattctgttt atcgcttctg gcgcgttcca
    11521 gattgcgaaa ccgtctgacc tgatcccgga actgcaaggt cgtctgccaa tccgcgttga
    11581 gctgcaggca ctgaccacca gcgacttcga gcgtattctg accgagccga atgcctctat
    11641 cactgtgcag tacaaagcac tgatggcaac tgaaggcgta aatatcgagt ttaccgactc
    11701 cggtattaag cgcatcgcgg aagcggcatg gcaggtgaac gaatctaccg aaaacatcgg
    11761 tgctcgtcgt ttacataccg ttctggagcg tttaatggaa gagatttcct acgacgccag
    11821 cgatttaagc ggtcaaacta tcactattga cgcagattat gtgagcaaac atctggatgc
    11881 gttggtggca gatgaagatc tgagccgttt tatcctataa tcgcgttcaa tcattttcat
    11941 cattgtttga tggggctgaa aggccccatt tttattggcg cgtattatga ctgaacaaca
    12001 aattagccga actcaggcgt ggctggaaag tttacgacct aaaaccctcc ccctcgcctt
    12061 tgctgcaatt atcgtcggga cggcgctggc atggtggcaa ggtcacttcg atccactggt
    12121 cgccctgctg gcattgatta ccgccgggct attacagatc ctttctaacc tcgccaatga
    12181 ttacggcgat gcggtaaaag gcagcgataa acctgaccgc attgggccgc tacgcggcat
    12241 gcaaaaaggg gtcattaccc agcaagagat gaaacgggcg ctcattatta ccgttgtgct
    12301 catctgtctt tccgggctgg cactggttgc agtggcgtgc catacgctgg ccgattttgt
    12361 cggtttcctg attcttggcg ggttgtcgat cattgccgct atcacctaca ccgtgggcaa
    12421 tcgtccttat ggttatatcg gtttgggtga tatttccgta ctggttttct ttggctggtt
    12481 gagcgtcatg gggagctggt atttacaggc tcatacattg attccggcac tgatccttcc
    12541 ggcaaccgca tgcggcctgc tggcaacggc agtactgaac attaataacc tgcgtgatat
    12601 caatagcgac cgcgaaaacg gcaaaaacac gctggtggtg cgcttaggtg cagtaaacgc
    12661 gcgtcgttat catgcctgcc tgctgatggg ctcactggtg tgtctggcgc tgtttaatct
    12721 cttttcgctg catagcctgt ggggctggct gttcctgctg gcggcaccgt tgctggtgaa
    12781 gcaagctcgt tatgtgatgc gcgaaatgga cccggtggcg atgcgaccaa tgctggaacg
    12841 cactgtcaaa ggagcgttac tgactaacct gctgtttgtt ttagggatat tcctaagcca
    12901 gtgggcagca taactgacaa atatcaatta acaattgatg attttgccaa cagcccacat
    12961 agcgcgatat actgaaaatt ctcgcagcaa ctgaatgtta agcctatgaa atacgatact
    13021 tccgagcttt gtgacatcta tcaagaagat gttaacgtcg tggaaccgct gttctccaac
    13081 tttggcggac gggcgtcgtt tggcggacaa ataatcacgg taaaatgttt cgaggacaac
    13141 gggttgctgt acgatctgct cgaacagaat ggccgtggtc gtgttcttgt cgtcgatggc
    13201 ggaggttctg ttcgtcgcgc actggtcgat gctgaactgg cgcgtctggc agtacaaaat
    13261 gaatgggaag gtctggtcat ttacggcgcg gtgcgtcagg tagatgacct ggaagagttg
    13321 gatatcggca tccaggcgat ggcggcaatt ccggttggtg ccgctggcga aggcattggc
    13381 gaaagcgatg tccgcgtcaa ttttggcggt gtcaccttct tctccggcga ccatctttat
    13441 gccgacaata ccgggattat tctttcagaa gatccgctgg atattgaatg ataaaaaagg
    13501 caccgcaagg tgcctttttt ctgcgttacc tgttggccta cacagtaaag aaattacgcg
    13561 aaagatgaag tgtaatcaga cctcttccat gcgacccagc agggcctgta gacgttcctg
    13621 ccagccgttc tgctgttctt tcagatggtt gttctcacgc tccagctctt cgcgctgatg
    13681 ctgggcattt tgaacttcct gcgacagtga gttgtttttt tctttcagct cttcgatttc
    13741 catctgcaac agagtgatgg tatcaatcgc ctgctgtact tttgcttcca gtttctcaaa
    13801 cacttctaat gacattgtca tacctctcct gaattgcaag gcgttgatgg ataaaaatcc
    13861 tcgtcccgat taccggtgac gccttaataa atacgagcgc actttagtta gctccgattg
    13921 tatgaagccg cgccatcgct gtccagcggc acgccttgca gattacggtt tgccacactt
    13981 ttcatccttc tcctggtgac ataatccaca ccaatcgaaa atgttaataa atttgttgcg
    14041 cgaatgatct aacaaacatg catcatgtac aatcagatgg aataaatggc gcgataacgc
    14101 tcattttatg acgaagcaca cacattttaa gttcgatatt tctcgttttt gctcgttaac
    14161 gataagttta cagcatgcct acaagcatcg tggaggtccg tgactttcac gcatacaaca
    14221 aacattaact cttcaggatc cgattatgag tcaaacatca accttgaaag gccagtgcat
    14281 tgctgaattc ctcggtaccg ggttgttgat ttttttcggt gtgggttgcg ttgcagcact
    14341 aaaagtcgct ggtgcgtctt ttggtcagtg ggaaatcagt gtcatttggg gactgggggt
    14401 ggcaatggcc atctacctga ccgcaggggt ttccggcgcg catcttaatc ccgctgttac
    14461 cattgcattg tggctgtttg cctgtttcga caagcgcaaa gttattcctt ttatcgtttc
    14521 acaagttgcc ggcgctttct gtgctgcggc tttagtttac gggctttact acaatttatt
    14581 tttcgacttc gagcagactc atcacattgt tcgcggcagc gttgaaagtg ttgatctggc
    14641 tggcactttc tctacttacc ctaatcctca tatcaatttt gtgcaggctt tcgcagttga
    14701 gatggtgatt accgctattc tgatggggct gatcctggcg ttaacggacg atggcaacgg
    14761 tgtaccacgc ggccctttgg ctcccttgct gattggtcta ctgattgcgg tcattggcgc
    14821 atctatgggc ccattgacgg gctttgccat gaacccagcg cgtgacttcg gtccgaaagt
    14881 ctttgcctgg ctggcgggct ggggcaatgt cgcctttacc ggcggcagag acattcctta
    14941 cttcctggtg ccgctttttg gccctatcgt tggcgcgatt gtaggtgcat ttgcctaccg
    15001 caaactgatt ggtcgccatt tgccttgcga tatctgtgtt gtggaagaaa aggagaccac
    15061 aactccttca gaacaaaaag cttcgctgta atatgactac gggacaatta aacatgactg
    15121 aaaaaaaata tatcgttgcg ctcgaccagg gcaccaccag ctcccgcgcg gtcgtaatgg
    15181 atcacgatgc caatatcatt agcgtgtcgc agcgcgaatt tgagcaaatc tacccaaaac
    15241 caggctgggt agaacacgac ccaatggaaa tctgggccac ccaaagctcc acgctggtag
    15301 aagtgctggc gaaagccgat atcagttccg atcagattgc agctatcggt attacgaacc
    15361 agcgtgaaac cactattgtc tgggaaaaag aaaccggcaa gcctatctat aacgccattg
    15421 tctggcagtg ccgtcgtacc gcagaaatct gcgagcattt aaaacgtgac ggtttagaag
    15481 attatatccg cagcaatacc ggtctggtga ttgacccgta tttctctggc accaaagtga
    15541 agtggatcct cgaccatgtg gaaggctctc gcgagcgtgc gcgtcgtggt gaattgctgt
    15601 ttggtacggt tgatacgtgg cttatctgga aaatgactca gggccgtgtc catgtgaccg
    15661 attacaccaa cgcctctcgt accatgttgt tcaacatcca taccctggac tgggacgaca
    15721 aaatgctgga ggtgctggat attccgcgtg agatgctgcc agaagtgcgt cgttcttccg
    15781 aagtatacgg tcagactaac attggcggca aaggcggcac gcgtattcca atctccggga
    15841 tcgccggtga ccagcaggcc gcgctgtttg gtcagttgtg cgtgaaagaa gggatggcga
    15901 agaataccta cggcactggc tgctttatgc tgatgaacac cggcgagaaa gcggtgaaat
    15961 cagaaaacgg cctgctgacc accatcgcct gcggcccgac tggcgaagtg aactatgcgc
    16021 tggaaggtgc agtgtttatg gcgggcgcgt ccattcagtg gctgcgcgat gagatgaagt
    16081 tgattaacga cgcctacgat tccgaatatt tcgccaccaa agtgcaaaac accaatggtg
    16141 tgtatgtggt tccggccttt accgggctgg gtgcgccata ctgggatccg tatgcacgcg
    16201 gggcgatttt cggtctgact cgtggggtga acgctaacca cattatacgc gcgacgctgg
    16261 agtctattgc ttatcagacg cgtgacgtgc tggaagcaat gcaggccgac tccggtattc
    16321 gtctgcacgc gctgcgcgtg gacggcggtg cggtggcgaa caatttcctg atgcagttcc
    16381 agtccgatat tctcggtacg cgcgttgagc gcccggaagt gcgcgaagtc accgctttgg
    16441 gtgcggccta tcttgctggt ctggcggttg gcttctggca gaatcttgac gagctgcaag
    16501 agaaagcggt gattgaacgc gagttccgtc ctggcatcga aaccactgag cgtaattacc
    16561 gttacgcagg ctggaaaaaa gcggtcaaac gcgcgatggc atgggaagaa cacgacgagt
    16621 aataatga
//
LOCUS       DADSYM010000109        16450 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2415
            SAMN08039579-rid11723173.denovo.109, whole genome shotgun sequence.
ACCESSION   DADSYM010000109 DADSYM010000000
VERSION     DADSYM010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039579
            Sequence Read Archive: SRR6362533
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16450)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16450)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980162.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 03:39:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,484
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 4,347
            CDSs (with protein)               :: 4,347
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 43 of 85
            Pseudo Genes (incomplete)         :: 37 of 85
            Pseudo Genes (internal stop)      :: 19 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16450
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039579-rid11723173.denovo.109"
                     /strain="LGE2415"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8302 N 95.9628 W"
                     /collection_date="2015-08-24"
     gene            complement(83..163)
                     /gene="shoB"
                     /locus_tag="J1Y83_003100"
     CDS             complement(83..163)
                     /gene="shoB"
                     /locus_tag="J1Y83_003100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin ShoB"
                     /protein_id="HBA7631227.1"
                     /translation="MTDCRYLIKRVIKIIIAVLQLLLLFL"
     gene            complement(584..964)
                     /locus_tag="J1Y83_003101"
     CDS             complement(584..964)
                     /locus_tag="J1Y83_003101"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HBA7631228.1"
                     /translation="MAILGLGTDIVEIARIEAVIARSGERLARRVLSDNEWEIWKTHH
                     QPVRFLAKRFAVKEAAAKAFGTGIRNGLAFNQFEVFNDELGKPRLRLWGEALKLAEKL
                     GVVNMHVTLADERHYACATVIIES"
     gene            complement(964..1695)
                     /gene="pdxJ"
                     /locus_tag="J1Y83_003102"
     CDS             complement(964..1695)
                     /gene="pdxJ"
                     /locus_tag="J1Y83_003102"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HBA7631229.1"
                     /translation="MAELLLGVNIDHIATLRNARGTAYPDPVQAAFIAEQAGADGITV
                     HLREDRRHITDRDVRILRQTLDTRMNLEMAVTEEMLAIAVETKPHFCCLVPEKRQEVT
                     TEGGLDVAGQRDKMRDACKRLAEAGIQVSLFIDADEEQIKAAAEVGAPFIEIHTGCYA
                     DAKTDAEQAQELARIAKAATFAASLGLKVNAGHGLTYHNVKAIAAIPEMHELNIGHAI
                     IGRAVMTGLKDAVAEMKRLMLEARG"
     gene            complement(1707..2435)
                     /gene="recO"
                     /locus_tag="J1Y83_003103"
     CDS             complement(1707..2435)
                     /gene="recO"
                     /locus_tag="J1Y83_003103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HBA7631230.1"
                     /translation="MEGWQRAFVLHSRPWSETSLMLDVFTEESGRVRLVAKGARSKRS
                     TLKGALQPFTPLLLRFGGRGEVKTLRSAEAVSLALPLSGITLYSGLYINELLSRVLEY
                     ETRFSELFFDYLHCIQSLAGATGTPEPALRRFELALLGHLGYGVNFTHCAGSGEPVDD
                     TMTYRYREEKGFIASVVIDNKTFTGRQLKALNAREFPDADTLRAAKRFTRMALKPYLG
                     GKPLKSRELFRQFMPKRTVKTHYE"
     gene            complement(2447..3352)
                     /gene="era"
                     /locus_tag="J1Y83_003104"
     CDS             complement(2447..3352)
                     /gene="era"
                     /locus_tag="J1Y83_003104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HBA7631231.1"
                     /translation="MSIDKSYCGFVAIVGRPNVGKSTLLNKLLGQKISITSRKAQTTR
                     HRIVGIHTEGAYQAIYVDTPGLHMEEKRAINRLMNKAASSSIGDVELVIFVVEGTRWT
                     PDDEMVLNKLRDGKAPVILAVNKVDNVQEKADLLPHLQFLASQMNFLDIVPISAETGL
                     NVDTIAAIVRKHLPEATHHFPEDYITDRSQRFMASEIIREKLMRFLGAELPYSVTVEI
                     ERFVSNERGGYDINGLILVEREGQKKMVIGNKGAKIKTIGIEARKDMQEMFEAPVHLE
                     LWVKVKSGWADDERALRSLGYVDDL"
     gene            complement(3349..4029)
                     /gene="rnc"
                     /locus_tag="J1Y83_003105"
     CDS             complement(3349..4029)
                     /gene="rnc"
                     /locus_tag="J1Y83_003105"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003848606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HBA7631232.1"
                     /translation="MNPIVINRLQRKLGYTFNHQELLQQALTHRSASSKHNERLEFLG
                     DSILSYVIANALYHRFPRVDEGDMSRMRATLVRGNTLAELAREFELGECLRLGPGELK
                     SGGFRRESILADTVEALIGGVFLDSDIQTVEKLILNWYQTRLDEISPGDKQKDPKTRL
                     QEYLQGRHLPLPTYLVVQVRGEAHDQEFTIHCQVSGLSEPVVGTGSSRRKAEQAAAEQ
                     ALKKLELE"
     gene            complement(4301..5275)
                     /gene="lepB"
                     /locus_tag="J1Y83_003106"
     CDS             complement(4301..5275)
                     /gene="lepB"
                     /locus_tag="J1Y83_003106"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HBA7631233.1"
                     /translation="MANMFALILVIATLVTGILWCVDKFFFAPKRRERQAAAQAAAGD
                     SLDKATLKKVAPKPGWLETGASVFPVLAIVLIVRSFIYEPFQIPSGSMMPTLLIGDFI
                     LVEKFAYGIKDPIYQKTLIETGHPKRGDIVVFKYPEDPKLDYIKRAVGLPGDKVTYDP
                     VSKELTIQPGCSSGQACENALPVTYSNVEPSDFVQTFSRRNGGEATSGFFEVPKNETK
                     ENGIRLSERKETLGDVTHRILTVPIAQDQVGMYYQQPGQQLATWIVPPGQYFMMGDNR
                     DNSADSRYWGFVPEANLVGRATAIWMSFDKQEGEWPTGVRLSRIGGIH"
     gene            complement(5291..7090)
                     /gene="lepA"
                     /locus_tag="J1Y83_003107"
     CDS             complement(5291..7090)
                     /gene="lepA"
                     /locus_tag="J1Y83_003107"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010434076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HBA7631234.1"
                     /translation="MKNIRNFSIIAHIDHGKSTLSDRIIQICGGLSDREMEAQVLDSM
                     DLERERGITIKAQSVTLDYKASDGETYQLNFIDTPGHVDFSYEVSRSLAACEGALLVV
                     DAGQGVEAQTLANCYTAMEMDLEVVPVLNKIDLPAADPERVAEEIEDIVGIDATDAVR
                     CSAKTGVGVQDVLERLVRDIPPPEGDPEGPLQALIIDSWFDNYLGVVSLIRIKNGTLR
                     KGDKVKVMSTGQTYNADRLGIFTPKQVDRTELKCGEVGWLVCAIKDIHGAPVGDTLTL
                     ARNPAEKALPGFKKVKPQVYAGLFPVSSDDYEAFRDALGKLSLNDASLFYEPESSSAL
                     GFGFRCGFLGLLHMEIIQERLEREYDLDLITTAPTVVYEVETTSREVIYVDSPSKLPA
                     VNNIYELREPIAECHMLLPQAYLGNVITLCVEKRGVQTNMVYHGNQVALTYEIPMAEV
                     VLDFFDRLKSTSRGYASLDYNFKRFQASDMVRVDVLINGERVDALALITHRGNSQNRG
                     RELVEKMKDLIPRQQFDIAIQAAIGTHIIARSTVKQLRKNVLAKCYGGDISRKKKLLQ
                     KQKEGKKRMKQIGNVELPQEAFLAILHVGKDNK"
     gene            complement(7288..7767)
                     /gene="rseC"
                     /locus_tag="J1Y83_003108"
     CDS             complement(7288..7767)
                     /gene="rseC"
                     /locus_tag="J1Y83_003108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SoxR-reducing system protein RseC"
                     /protein_id="HBA7631235.1"
                     /translation="MIKEWATVVSWENGQALVSCDVKASCSSCASRAGCGSRVLNKLG
                     PQTTHTIVVPCDEPLVPGQKVELGIAEGSLLSSALLVYMSPLVGLFLIASLFQLLFAS
                     DVAALCGAILGGIGGFLIARGYSRKFAARAEWQPIILSVALPPGLVRFETSSEDASQ"
     gene            complement(7764..8720)
                     /gene="rseB"
                     /locus_tag="J1Y83_003109"
     CDS             complement(7764..8720)
                     /gene="rseB"
                     /locus_tag="J1Y83_003109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-E factor regulatory protein RseB"
                     /protein_id="HBA7631236.1"
                     /translation="MKQLWFAMSLVTGSLLFSANASATPASGALLQQMNLASQSLNYE
                     LSFISINKQGVESLRYRHARLDNRPLAQLLQMDGPRREVVQRGNEISYFEPGLEPFTL
                     NGDYIVDSLPSLIYTDFKRLSPYYDFISVGRTRIADRLCEVIRVVARDGTRYSYIVWM
                     DTESKLPMRVDLLDRDGETLEQFRVIAFNVNQDIGSSMQTLAKANLPPLLSVPVGEKA
                     KFSWTPTWLPQGFSEVSSSRRPLPTMDNMPIESRLYSDGLFSFSVNVNRATPSSTDQM
                     LRTGRRTVSTSVRDNAEITIVGELPPQTAKRIAENIKFGAAQ"
     gene            complement(8720..9370)
                     /gene="rseA"
                     /locus_tag="J1Y83_003110"
     CDS             complement(8720..9370)
                     /gene="rseA"
                     /locus_tag="J1Y83_003110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma-E factor RseA"
                     /protein_id="HBA7631237.1"
                     /translation="MQKEQLSALMDGETLDSELLNELAHNPEMQKTWESYHLIRDSMR
                     GDTPEVLHFDISSRVMAAIEEEPVRQPATLIPEAQPAPHQWQKMPFWQKVRPWAAQLT
                     QMGVAACVSLAVIVGVQHYNGQSETSQQPETPVFNTLPMMGKASPVSLGVPSEATANN
                     GQQQQVQEQRRRINAMLQDYELQRRLHSEQLQFEQAQTQQAAVQVPGIQTLGTQSQ"
     gene            complement(9403..9978)
                     /gene="rpoE"
                     /locus_tag="J1Y83_003111"
     CDS             complement(9403..9978)
                     /gene="rpoE"
                     /locus_tag="J1Y83_003111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005070454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoE"
                     /protein_id="HBA7631238.1"
                     /translation="MSEQLTDQVLVERVQKGDQKAFNLLVVRYQHKVASLVSRYVPSG
                     DVPDVVQEAFIKAYRALDSFRGDSAFYTWLYRIAVNTAKNYLVAQGRRPPSSDVDAIE
                     AENFESGGALKEISNPENLMLSEELRQIVFRTIESLPEDLRMAITLRELDGLSYEEIA
                     AIMDCPVGTVRSRIFRAREAIDNKVQPLIRR"
     gene            complement(9975..10130)
                     /gene="rseD"
                     /locus_tag="J1Y83_003112"
     CDS             complement(9975..10130)
                     /gene="rseD"
                     /locus_tag="J1Y83_003112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rpoE leader peptide RseD"
                     /protein_id="HBA7631239.1"
                     /translation="MIRLQHDKQKQMRYGTLQKRDTLTLCLLKLQLMEWRFDSAWKFG
                     LGRLYLG"
     gene            10386..12008
                     /gene="nadB"
                     /locus_tag="J1Y83_003113"
     CDS             10386..12008
                     /gene="nadB"
                     /locus_tag="J1Y83_003113"
                     /EC_number="1.4.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
                     /protein_id="HBA7631240.1"
                     /translation="MNTLPEHSCDVLIIGSGAAGLSLALRLADQHQVIVLSKGPVTEG
                     STFYAQGGIAAVFDETDSIDSHVEDTLIAGAGICDRHAVEFVASNARSCVQWLIDQGV
                     LFDTHIQPNGEESYHLTREGGHSHRRILHAADATGREVETTLVGKAQNHPNIRVLERS
                     NAVDLIVSDKIGLPGTRRVVGAWVWNRNKETVETCHAKAVVLATGGASKVYQYTTNPD
                     ISSGDGIAMAWRAGCRIANLEFNQFHPTALYHPQARNFLLTEALRGEGAYLKRPDGTR
                     FMPDFDERGELAPRDIVARAIDHEMKRLGADCMFLDISHKPADFIRQHFPMIYEKLLG
                     LGIDLTKEPVPIVPAAHYTCGGVMVDDHGRTDVEGLYAIGEVSYTGLHGANRMASNSL
                     LECLVYGWSAAEDISRRIPYAHGVSTLPPWDESRVENPDERVVIQHNWHELRLFMWDY
                     VGIVRTTKRLERALRRITMLQQEIDEYYAHFRVSNNLLELRNLVQVAELIVRCAMMRK
                     ESRGLHFTLDYPELLTHSGPSILSAGNHYINR"
     gene            complement(11993..12730)
                     /gene="trmN"
                     /locus_tag="J1Y83_003114"
     CDS             complement(11993..12730)
                     /gene="trmN"
                     /locus_tag="J1Y83_003114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417070.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA(1)(Val)
                     (adenine(37)-N(6))-methyltransferase TrmN"
                     /protein_id="HBA7631241.1"
                     /translation="MSQSTSVFRRNGFTFKQFFVAHDRCAMKVGTDGILLGAWAPVAG
                     VKRCLDIGAGSGLLALMLAQRTDDSVMIDAVELESEAAAQAQENINQSPWAERINVHT
                     ADILQWITQQTVRFDLIISNPPYYQQGVECATPQREQARYTTTLDHPSLLTCAAECIT
                     EEGFFCVVLPEQIGNGFTELALSMGWHLRLRTDVAENEARLPHRVLLAFSPQAGECFS
                     DRLVIRGPDQNYSEAYTALTQAFYLFM"
     gene            12862..14196
                     /gene="srmB"
                     /locus_tag="J1Y83_003115"
     CDS             12862..14196
                     /gene="srmB"
                     /locus_tag="J1Y83_003115"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase SrmB"
                     /protein_id="HBA7631242.1"
                     /translation="MTVTTFSELELDESLLEALQDKGFTRPTAIQAAAIPPALDGRDV
                     LGSAPTGTGKTAAYLLPALQHLLDFPRKKSGPPRILILTPTRELAMQVADHARELAKH
                     THLDIATITGGVAYMNHAEVFSENQDIVVATTGRLLQYIKEENFDCRAVETLILDEAD
                     RMLDMGFAQDIEHIAGETRWRKQTLLFSATLEGDAIQDFAERLLEDPVEVSANPSTRE
                     RKKIHQWYYRADDLEHKTALLVHLLKQPEATRSIVFVRKRERVHELANWLREAGINNC
                     YLEGEMVQGKRNEAIKRLTEGRVNVLVATDVAARGIDIPDVSHVFNFDMPRSGDTYLH
                     RIGRTARAGRKGTAISLVEAHDHLLLGKVGRYIEEPIKARVIDELRPKTRAPSEKQTG
                     KPSKKVLAKRAEKKKAKEKEKPRVKKRHRDTKNIGKRRKPSGTGVPPQTTEE"
     gene            complement(14229..15110)
                     /locus_tag="J1Y83_003116"
     CDS             complement(14229..15110)
                     /locus_tag="J1Y83_003116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBA7631243.1"
                     /translation="MDLRRFITLKTVVEEGSFLRASQKLCCTQSTVTFHIQQLEQEFS
                     VQLFEKIGRRMCLTREGKKLLPHIYELTRVMDTLREAAKKESDPDGELRVISGETLLS
                     YRMPQVLQRFRQRAPKVRLSLQSLNCYVIRDALLNDEADVGVFYRVGNDDALNRRELG
                     EQPLALVASPQIADVDFTEPGRHNACSFIINEPQCVFRQIFESTLRQRRITVENTIEL
                     ISIESIKRCVAANIGVSYLPRFAVVKELECGELIELPFGEQSQTITAMCAHHAGKAVS
                     PAMHTFIQCVEECFLPG"
     gene            15213..15800
                     /gene="eamB"
                     /locus_tag="J1Y83_003117"
     CDS             15213..15800
                     /gene="eamB"
                     /locus_tag="J1Y83_003117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/O-acetylserine transporter"
                     /protein_id="HBA7631244.1"
                     /translation="MTPTLLSAFWTYTLITAMTPGPNNILALSSATSHGFRQSTRVLA
                     GMSLGFLIVMLLCAGISFSLTVIDPAAVHLLSWAGAAYIVWLAWKIATSPTKEDGLQT
                     KPISFWASFALQFVNVKIILYGVTALSTFVLPQTQALSWVVGVSVLLAMIGTFGNVCW
                     ALAGHLFQRLFRQYGRQLNIVLALLLVYCAVRIFY"
     gene            complement(15856..16239)
                     /gene="grcA"
                     /locus_tag="J1Y83_003118"
     CDS             complement(15856..16239)
                     /gene="grcA"
                     /locus_tag="J1Y83_003118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autonomous glycyl radical cofactor GrcA"
                     /protein_id="HBA7631245.1"
                     /translation="MITGIQITKAANDDLLNSFWLLDSEKGEARCIVAKAGFAEDEVV
                     AVSKLGDIEYREVPVEVKPEVRVEGGQHLNVNVLRRETLEDAVKHPEKYPQLTIRVSG
                     YAVRFNSLTPEQQRDVIARTFTESL"
BASE COUNT         3990 a         4364 c         4030 g         4066 t
ORIGIN      
        1 tatctgccca gacacttatt tatagttatt aaaggcgcgt ccgattggtt caccggacgc
       61 accttaagta cgtttccttg tgttataaga acaaaaggag cagctgtaaa acagcaatga
      121 tgattttgat gacccgttta atcaggtatc ggcaatcagt cattcgtttt tccttaaaca
      181 aggaatgcag ccgttcagat tagcccttac atctccccaa aactgaacgt gcgagttatt
      241 ggaatcgagt gctgcactcc acaccagagc tttgacgaca ccactcgttt caatggggga
      301 attcagtggc atggtgtaaa gcacagcaaa attgccaata acgaaaccga ttttaatgta
      361 cttatcaaaa agcggtcacg ttaatctttt catcaatgtc gatttcttcg taaaggctcg
      421 cgttttttat gtcagagatt gcaagttgct tgtacaggtc aacgaaattg atccataatc
      481 attttcgttg gaatcgtgtg ctgcacaaaa ttaaagttaa aaagtaaaac ccccgttcct
      541 taccagttcg ggggttttac tttataaaga gaacggtatt attttaactt tcaataatta
      601 ccgtggcaca agcatagtgg cgctcatcgg caagcgttac atgcatattt acaacgccga
      661 gcttttccgc cagttttaat gcctcacccc atagccgtag ccgtggtttg ccgagctcat
      721 cattgaatac ttcaaactga ttaaacgcca gaccattgcg gatccctgtg ccaaatgctt
      781 ttgccgccgc ttctttcaca gcaaaacgct tcgccagaaa acgcactggc tggtggtgcg
      841 ttttccagat ttcccattcg ttatcgctta atacgcggcg tgccaggcgt tcaccggatc
      901 gggcgatcac cgcttctatg cgagcgatct ccacaatatc cgtacctaaa cctaatattg
      961 ccattagcca cgcgcttcca gcatcagacg cttcatttct gccaccgcat ctttcagtcc
     1021 ggtcatcact gcacgaccaa taatggcatg accgatattc agttcatgca tctcagggat
     1081 ggcagcaata gctttcacgt tgtgataggt cagaccgtga ccagcgttaa ctttcagacc
     1141 gagactggcg gcaaaggtcg cggctttggc gatacgcgcc agctcttgcg cctgttcggc
     1201 gtcagttttg gcatcagcat agcaacctgt gtggatctcg ataaacggtg cgccaacctc
     1261 tgccgcagct ttgatctgct cttcatcggc gtcgataaac agagaaacct ggatcccggc
     1321 ctccgccaga cgtttacagg catcgcgcat tttgtcacgc tgccctgcga cgtccaggcc
     1381 gccttcggtt gttacttcct gacgcttttc cggtaccaga cagcaaaaat gtggcttcgt
     1441 ctcaacggcg atcgccagca tctcttcggt caccgccatc tccagattca tgcgggtatc
     1501 cagcgtctga cgcaggatgc gcacatcgcg gtcggtaatg tggcggcgat cttcacgtaa
     1561 atgcacggta atgccgtctg ctcccgcctg ctcggcaata aacgccgcct gcaccggatc
     1621 cgggtaagca gtaccgcgtg cgttacgcag cgttgcaata tggtcaatgt tgacgcctaa
     1681 cagtaattca gccatgacaa tcctcatcat tcataatgtg ttttcaccgt tcgcttaggc
     1741 ataaactgcc ggaacagttc cctgctcttt aatggtttac cgccaagata cggcttaagc
     1801 gccatgcggg taaagcgttt cgcggcgcgc agtgtgtctg cgtcaggaaa ttcccgcgcg
     1861 tttaacgctt ttaactgcct tccggtgaac gttttattgt cgataacgac gcttgcgata
     1921 aacccttttt cttcgcgata acgatacgtc atggtgtcat ctaccggctc gccgctaccc
     1981 gcacaatggg taaaattgac gccatagccc agatgcccga gcagtgccag ttcaaagcgg
     2041 cgcagcgcgg gttctggcgt accagtggca cctgcaagtg actgaatgca gtgcaagtaa
     2101 tcgaaaaaga gttcagagaa gcgcgtctcg tattccagta cgcgggagag aagttcgttg
     2161 atgtacagac cgctgtaaag cgtgataccg cttaatggca gcgccagcga gacggcttca
     2221 gcactgcgca gcgtttttac ttcgccacgc ccgccgaaac gtagcaagag aggggtgaaa
     2281 ggctgtaatg cacctttcag agtagagcgt ttagagcgtg cgcctttggc aaccagacgc
     2341 acgcgacccg attcctccgt gaagacgtcc agcatcaggc tggtttcgct ccacgggcga
     2401 ctatgcagga caaatgcgcg ctgccagcct tccatcggag ttactcttaa agatcgtcaa
     2461 cgtaaccgag actgcgcagt gcgcgttcgt cgtcggccca accggatttc acttttaccc
     2521 acagctcaag gtgaacaggc gcttcgaaca tttcctgcat gtctttacgc gcttcaatcc
     2581 cgatggtttt gatcttggcc cctttgttgc caataaccat cttcttctgc ccttcacgct
     2641 caacgagaat caaaccgttg atgtcataac cgccgcgttc gttagagacg aaacgttcga
     2701 tctccacggt cacggagtac ggcagttcag cgccgagaaa acgcatcagt ttttcgcgga
     2761 tgatttcaga cgccataaaa cgctgtgagc gatcggtgat gtaatcttcc gggaagtgat
     2821 gagtcgcttc aggcagatgc ttacgcacga ttgccgcgat agtgtcgaca ttcagcccgg
     2881 tttcggcaga gatcggcacg atatcgagga agttcatctg gcttgccagg aactgtaagt
     2941 gtggcagcag atcggctttt tcctgcacgt tgtccacttt gttcaccgcg aggattaccg
     3001 gcgctttgcc atcacgcagc ttgttgagca ccatttcatc gtccggcgtc cagcgggtgc
     3061 cttctacgac aaaaatcacc agctcaacgt cgccaatgga gctgctcgcc gctttgttca
     3121 tcagacggtt aatagcgcgt ttttcttcca tatgcaggcc tggggtatca acgtagatcg
     3181 cctgatacgc gccttcagta tggatcccca caatgcggtg acgagttgtt tgcgccttgc
     3241 gggaggtaat ggagatcttc tgccccagca gtttgttcaa caatgtggat ttgccaacgt
     3301 tcggacgtcc gacgatagca acaaatccgc agtaactttt atcgatgctc attccagctc
     3361 cagttttttc aacgcctgtt cggcggcagc ctgctcagcc ttacgacggc ttgaacctgt
     3421 gccaaccacc ggttcactca ggccgctgac ctggcagtgg atagtaaatt cctgatcgtg
     3481 cgcttcgcca cgtacctgga ctaccagata agttggcagc ggcagatggc gaccctgcaa
     3541 atattcctgc agacgcgttt tcggatcttt ttgtttatcg cctgggctaa tttcgtccag
     3601 acgagtttga taccagttga ggattaattt ctcgacggtt tgaatatcac tgtcgaggaa
     3661 tacgccacca attaatgctt cgacggtgtc ggcgagaatt gactcacgac gaaatccacc
     3721 gcttttaagt tcacctggcc ctaaacgtaa gcactcacct aactcaaatt cgcgcgccag
     3781 ttcagccagc gtattaccac ggaccagcgt ggcgcgcatc cggctcatat cgccttcatc
     3841 cacacgaggg aaacggtgat aaagcgcatt ggcgataacg tagctcagaa tagagtcacc
     3901 taaaaattct aaacgctcgt tatgtttact gctggcacta cgatgagtta atgcctgctg
     3961 caacagttcc tgatgattaa aagtgtagcc cagcttccgt tgaagccgat taattacgat
     4021 ggggttcatg cgataccaat aaataaatgc gtcaacaatt cagcacacga aacagacctg
     4081 atatacatgg ctcggctaac tgcttcactg cagtttcagt ttaccggtat atggggacca
     4141 acgctgtttc gtgtgccgtg gcaacctgga ggtgccaacc ttaaacttcg ggggaatatt
     4201 ctatacacaa cgacggggga tgtcgttagc cacgggagat ttatctcata aataattcac
     4261 gttgtcgcca taacggcgac aacgtgaacg aagatggcta ttaatggata ccgccaatgc
     4321 gacttaagcg cacaccagtc ggccattcgc cttcttgctt atcgaagctc atccagatag
     4381 ccgttgcccg accgaccaga ttcgcttcag gcacaaagcc ccagtaacgg ctgtccgcgc
     4441 tgttgtcgcg gttgtcgccc atcatgaagt attgtcccgg cggaacaatc caggttgcca
     4501 gttgttgccc tggctgctgg taatacatcc ccacctggtc ctgcgcaatc ggcactgtca
     4561 ggatgcggtg cgtcacatca cccaatgtct ctttacgctc ggaaagacga attccatttt
     4621 ctttggtttc gtttttcggc acttcaaaga atccgctggt cgcttcccca ccattacggc
     4681 gtgagaaggt ctgaacgaaa tcgctcggtt ccacgttgga gtaggtgacc gggagcgcgt
     4741 tttcgcacgc ctggccggaa ctgcatcccg gttgaatcgt cagctctttt gagaccggat
     4801 cataagtgac tttatcgccc ggcaaaccca ccgcgcgctt gatgtaatca agctttggat
     4861 cttccggata tttaaagacc acgatatcgc cgcgtttcgg atgaccggtt tcgatcagcg
     4921 ttttctggta aataggatct ttaatgccat aagcaaactt ctctaccaga ataaaatcac
     4981 cgattaagag agtcggcatc atcgaacctg acgggatctg gaacggttca taaataaacg
     5041 aacgcacaat caatacgata gccagtaccg gaaaaacaga agcaccggtt tccagccagc
     5101 caggcttcgg cgcaaccttt ttcagcgttg ctttatccag tgagtcaccg gcagccgcct
     5161 gcgccgctgc ctgacgttcc cgccgtttag gtgcgaaaaa gaatttatcc acgcaccata
     5221 aaatgcccgt caccagtgtg gcaatcacca gaatcagggc aaacatattc gccatgccaa
     5281 ctcctaaggg ttatttgttg tctttgccga cgtgcagaat ggcgaggaac gcttcctgcg
     5341 gcagctcgac gttaccgatc tgcttcatgc gtttcttacc ttctttctgc ttctgcagca
     5401 gctttttctt acggctgata tcgccgccat aacatttagc cagtacgttt ttacgcagct
     5461 gtttcacggt ggatcgcgca ataatgtgcg taccaatcgc tgcctgaatg gcgatatcaa
     5521 actgctggcg tgggatcaga tctttcatct tctccaccag ctcgcgaccg cggttttgcg
     5581 aattaccacg gtgggtgatc agcgccaacg catcaacacg ttcaccgttg attaatacgt
     5641 ctacacgtac catgtcggac gcctggaagc gtttgaagtt gtaatccaga gacgcataac
     5701 cacgcgaggt agatttcagg cgatcgaaga agtcgagcac cacttccgcc atcgggatct
     5761 cgtacgtcag cgccacctga ttaccgtggt aaaccatatt ggtctgcacg ccgcgttttt
     5821 ctacgcacag cgtaataacg ttgccgagat atgcctgcgg tagcagcatg tgacactctg
     5881 caatcggctc gcgcagttcg taaatgttat ttaccgctgg cagcttggat gggctgtcga
     5941 cgtagataac ttctcttgac gtggtttcaa cttcatacac tacagtcggc gcagtggtga
     6001 tcagatccag atcgtattca cgttccagac gttcctggat gatctccatg tgcagcaggc
     6061 caaggaagcc gcagcggaaa ccaaagccca acgcgctgga gctttccggc tcatagaaca
     6121 gtgaggcatc gttcaggctg agtttaccca gcgcgtcacg gaaggcttca tagtcgtcgg
     6181 aacttaccgg gaacagacca gcatatacct gaggtttgac tttcttaaag ccaggcagcg
     6241 ccttttctgc cggattacgc gccagtgtta aggtatcgcc tactggagcg ccgtggatat
     6301 ctttaatcgc acataccagc cagcctactt cgccacattt cagttcagtg cggtcaacct
     6361 gcttcggcgt gaagatgccc agacgatcgg cgttataggt ctgcccggtg ctcatgactt
     6421 tcactttgtc gcccttacgc agggtgccgt ttttaatacg gataagtgaa acaacgccca
     6481 gatagttgtc gaaccaggag tcgataatta gtgcctgcaa cgggccttcc ggatcgcctt
     6541 ccggcggcgg aatgtcgcgc acaagacgtt cgagaacgtc ttgcacgcca acgccggttt
     6601 tcgctgaaca gcgcaccgcg tcggtggcgt cgatacctac gatatcttca atttcttccg
     6661 ccacgcgttc aggatcggct gccggcaggt caatcttgtt cagtaccggc accacttcga
     6721 gatccatttc catggcggtg tagcagtttg ccagggtttg cgcttctacg ccctgcccgg
     6781 cgtcgaccac cagcaatgca ccttcacagg cagccagcga acgggaaact tcataggaga
     6841 agtctacgtg gcccggggtg tcgataaagt taagctgata ggtttcgcca tcagacgctt
     6901 tgtagtccag cgtcacgctt tgcgctttga tggtaatgcc acgctcacgc tcaagatcca
     6961 tggaatcgag aacctgcgcc tccatttcac ggtcagacag gccaccgcag atctggataa
     7021 tacggtcaga cagcgtcgat ttaccgtggt caatgtgagc tatgatcgaa aagttacgta
     7081 tattcttcat aaagtatgat tattgtgcct taatgcccgg ttaaccaggc ttttagaagt
     7141 cgctgttctg agcttaacgt ctgtattaat agaaacgccg cattctacac tacaacattg
     7201 aggcgaggaa atgttcatac cgtatggatt gtggtatctg gaaacgtcct cgcatttgtt
     7261 atgcaaaatg caacaaagcc agtgaaatca ctggctcgcg tcttccgaag atgtttcaaa
     7321 tcgcacaagg ccaggcggca atgccacgct taagatgatc ggttgccatt ctgcccgggc
     7381 agcaaactta cgcgaatagc cgcgcgcaat caggaacccg ccaataccgc cgaggatcgc
     7441 accacataat gctgcgacgt cagaagcaaa gagtagctga aatagcgaag cgatgaggaa
     7501 taatcccacc agcggcgaca tataaaccag taatgcggag ctaagaaggc tgccttcggc
     7561 gatccccaat tccacttttt gccccggcac taacggctca tcacagggta cgacaatggt
     7621 atgcgtggtt tgcgggccaa gtttattcaa cacgcggcta ccgcaaccgg cgcgtgaagc
     7681 gcagctgctg catgaggctt taacatcgca actgaccagc gcctgcccgt tttcccagga
     7741 gacgacggta gcccactctt tgatcattgc gctgccccga acttaatatt ctcggcaatg
     7801 cgtttcgccg tttgcggcgg cagttcacca acaatggtga tttcggcgtt atcacgtacg
     7861 cttgtactga cggttctgcg cccggtgcgc aacatctgat cggtgctcga tggcgtagcg
     7921 cggttaacgt ttaccgagaa gctgaataat ccgtcggaat agagacgtga ttcgataggc
     7981 atgttgtcca tcgtcggtag cggacgtcga ctactggaaa cttcgctaaa accctgtggc
     8041 agccaggttg gcgtccagct gaatttagct ttttcaccga caggaacaga aagcaacggc
     8101 ggcaaatttg ccttcgccag cgtctgcata ctgctgccga tatcctgatt gacgttaaaa
     8161 gcaatcacgc gaaattgttc cagcgtttca ccatcgcgat caaggagatc aacccgcatc
     8221 ggtaatttcg attcggtgtc catccacacg atgtagctgt agcgtgtacc atctcgggca
     8281 accacgcgaa tgacttcgca aagacgatca gcaatacgcg tgcgcccgac ggagataaag
     8341 tcgtagtaag gagaaaggcg tttgaaatcg gtatagatga gcgatggcag agaatcaaca
     8401 atgtaatcgc cattaagcgt gaacggttca agtcccggtt caaaatagct gatttcattg
     8461 ccgcgctgta ccacttcccg gcgcgggcca tccatttgca acaattgtgc aagaggacgg
     8521 ttatcgaggc gtgcatgtcg ataacgcaga gactcaacac cctgtttatt gatgctgatg
     8581 aatgacagct cgtaattcag tgactgactg gccaggttca tctgctgtaa taacgccccg
     8641 gacgcgggag tggccgaggc gttagcagag aataacaggc tacctgtcac taatgacatg
     8701 gcaaaccaaa gttgcttcat tactgcgatt gcgttcctaa agtttgaatt cctggcacct
     8761 gtacagcggc ttgctgagtt tgtgcctgct caaactgaag ctgttcagag tggagtcggc
     8821 gttgcagttc gtaatcctgc aacattgcat taatgcgacg acgctgctcc tgtacctgct
     8881 gctgttgacc attatttgcg gtcgcttcag aaggtactcc cagactaacc gggctggctt
     8941 tacccatcat cggcagtgta ttaaatactg gcgtttcggg ctgctgggac gtttcagatt
     9001 gcccattata gtgctggacg ccaacgataa ctgcaagcga tacgcatgct gctacgccca
     9061 tttgggtaag ctgtgccgcc cacggacgta ctttctgcca gaatggcatt ttctgccatt
     9121 gatgcggcgc aggctgggct tccgggatca atgtcgccgg ttgacgtact ggctcttctt
     9181 caatggcggc catcacgcgt gaagagatat cgaaatggag cacctcggga gtatcacccc
     9241 gcattgagtc acggatcaag tgatagcttt cccaggtttt ctgcatttct gggttatgag
     9301 ccagttcgtt aagcagctca ctatccagcg tttcgccatc cattaaagcg gaaagttgtt
     9361 ctttctgcat gcctaatacc cttatccagt atcccgctat cgtcaacgcc tgataagcgg
     9421 ttgaactttg ttatcaatag cttccctcgc tcggaagata cgtgaacgca ccgtacctac
     9481 cggacaatcc atgatagcgg ctatctcttc atagctcagg ccatccagct cccgcaaggt
     9541 tattgccatg cgtaaatctt ccgggaggga ctcaatagtt cggaaaacta tctgtctcag
     9601 ttcttctgac aacattaagt tctcagggtt cgaaatttct ttcaacgcgc cgccactttc
     9661 gaagttttca gcttcaatgg catccacatc actggaaggt ggacgacgcc cctgagcaac
     9721 caggtaattt ttcgctgtat ttacagcaat ccgatacagc catgtataaa aagcgctatc
     9781 tccccggaac gaatccagcg cacgataggc tttaataaaa gcttcttgta ccacatcggg
     9841 aacatcaccc gacggcacat agcgggaaac cagactcgcc actttatgct gatagcgcac
     9901 taccagtaag ttaaaggctt tctgatctcc cttctggacc cgttcaacca ggacctggtc
     9961 cgttaactgc tcgctcatcc gaggtaaagt ctccccaaac caaatttcca cgcgctatcg
    10021 aaacgccact ccattagctg caatttgagc aagcaaaggg ttagagtgtc tcgtttttgt
    10081 aaagttccgt aacgcatctg tttttgtttg tcatgctgta gacggatcat tatctatcat
    10141 tataagtcta cagaatctga acatcgcatt atctgtgtag aaatgcccat ttaactgcct
    10201 gaagagtaac ccaacggcct ttttatttca ccacctaatc ctccaccagc cagtaacttc
    10261 tcgttttctc accgccctga ggcagcgtgt ttagtcactg taacaaatat taaaatagca
    10321 ggtgtttatt cgcacaacat gatgttatgc tgaccaaacc gtgtttagta aattaaacaa
    10381 agaaaatgaa tactctccct gaacattcat gtgacgtgtt gattatcggt agcggcgcag
    10441 ccggactttc actggcgctg cgcctggctg accagcatca ggtcatcgtt ctaagtaaag
    10501 gcccggtaac agaaggttca acattttatg cccagggcgg tattgccgcc gtgtttgatg
    10561 aaactgacag cattgactcg catgtggaag acacattgat tgccggggct ggtatttgcg
    10621 atcgccatgc agttgaattt gtcgccagta atgcacgatc ctgtgtgcaa tggctaatcg
    10681 accagggggt gttgtttgat acccacattc agccgaatgg cgaagaaagc taccatctga
    10741 ctcgtgaagg tggacatagt caccgtcgta ttcttcatgc cgccgacgcc accggtagag
    10801 aagtagaaac cacgctggtg ggcaaggcgc agaaccatcc gaatattcgc gtgctggagc
    10861 gcagcaacgc ggttgatctg attgtttctg acaaaattgg cctgccgggc acgcgacggg
    10921 ttgttggcgc gtgggtatgg aaccgaaata aagaaacggt tgaaacctgc cacgcaaaag
    10981 cagtggtact ggcaaccggc ggtgcttcaa aagtttatca gtacaccacc aatccggata
    11041 tttcttctgg cgatggcatt gctatggcgt ggcgcgcagg ctgccggatt gccaatctcg
    11101 aatttaatca gttccaccct accgcgctgt atcacccaca ggcacgcaat ttcctgttaa
    11161 cggaagcact gcgcggcgaa ggcgcttatc tcaagcgccc ggatggcacg cgttttatgc
    11221 ccgattttga tgagcgtggc gaactggctc cgcgcgatat tgtcgcccgc gccattgacc
    11281 atgagatgaa acgcctcggc gcggattgta tgttcctcga catcagccat aagcccgccg
    11341 attttattcg ccagcatttc ccgatgattt atgaaaaatt gctcgggctg gggatagatc
    11401 tcacgaaaga acccgtgccg attgtgcctg ctgcacatta tacctgcggt ggtgtaatgg
    11461 ttgatgatca tgggcgtacg gacgtcgagg gtttgtatgc cattggcgaa gtgagttata
    11521 ccggcttaca cggcgctaac cgcatggcct cgaattcatt gctggagtgt ctggtctatg
    11581 gctggtcggc ggcagaagat atcagcagac gtatacctta tgcccacggc gtcagtacgt
    11641 taccgccgtg ggatgaaagc cgcgttgaga accctgacga acgggtagta attcagcata
    11701 actggcacga gctacgtctg tttatgtggg attacgttgg cattgtgcgc acaacgaagc
    11761 gcctggaacg cgccctgcgg cggataacca tgctccaaca agaaatagac gaatattacg
    11821 cccatttccg cgtctcaaat aatttgctgg agctgcgtaa tctggtacag gttgccgagt
    11881 tgattgttcg ctgtgcgatg atgcgcaagg agagccgtgg cctgcatttc acgctggatt
    11941 atccggaact gctcacgcat tccggtccat cgatcctttc tgccggcaat cattacataa
    12001 acagataaaa agcctgggtc agcgccgtat acgcttcgga atagttctgg tctggcccac
    12061 gaatgactaa gcgatcgcta aagcattctc ccgcctgcgg ggagaatgcc agcagcaccc
    12121 gatgcggcag tcgcgcttcg ttttccgcca catccgtccg caaacgtaaa tgccagccca
    12181 tacttaatgc cagttcagta aaaccattac caatctgctc tggcagcacc acgcagaaaa
    12241 atccctcttc ggtaatgcac tccgccgcac aagtcagcaa cgatgggtga tcaagcgtag
    12301 tggtatagcg agcctgttcc cgttgaggtg tcgcgcactc tactccctgc tgatagtaag
    12361 gtgggttgct gatgattaaa tcgaagcgta ctgtctgctg tgtgatccac tgcagaatat
    12421 ccgccgtatg gacgttaatc cgctctgccc acggggactg gttgatattt tcctgcgctt
    12481 gcgcggcagc ttcactttcc agttcaactg catcaatcat cacgctgtca tctgttcgct
    12541 gcgccagcat taatgccaac aacccgctac ccgcgccgat atcaaggcaa cgttttaccc
    12601 cagccacagg tgcccatgcg cccagcaaaa tgccatccgt tcccactttc atcgcacagc
    12661 gatcgtgagc aacaaaaaac tgtttaaaag taaatccatt acgacgaaac acggatgtag
    12721 actgtgacat gaaaataaaa ccttgcagga aaaacggcga tagcaccggg tgagaacaat
    12781 acccgagaag cgatatccat acaaacagat gaagattgca gccgtaacgt ctataatcag
    12841 cgccccacac agaggtagaa catgactgta acgacttttt ccgaacttga actcgacgaa
    12901 agcctgctgg aagccctcca ggataaaggt ttcactcgcc cgaccgccat tcaggctgcc
    12961 gccattccgc ctgcgctcga tggccgtgat gtactcgggt ctgcgccgac aggcaccggt
    13021 aaaacggcgg cgtatctgct gccagcgttg cagcacctgc tcgatttccc acgtaagaaa
    13081 tccggtccgc cgcgtatttt gatccttacc ccgactcgcg aactggcgat gcaggtggcc
    13141 gatcatgccc gcgaactggc gaaacatacg catctggata ttgccaccat caccggcggc
    13201 gtggcctata tgaaccacgc agaagtgttc agcgaaaacc aggacattgt ggtcgccacg
    13261 accggacgtc tgctgcaata cataaaagaa gagaacttcg attgccgcgc ggttgaaacg
    13321 ctgatcctcg acgaagcaga ccgtatgctg gatatgggct tcgcccagga tatcgaacat
    13381 attgctggcg aaacgcgctg gcgtaaacag accctgctct tttcggcaac gctggaaggc
    13441 gatgcgattc aggattttgc cgagcgtctg ctggaagatc cggtggaagt ttctgcgaat
    13501 ccgtccaccc gtgagcgcaa aaaaattcat cagtggtatt accgcgccga tgatcttgag
    13561 cataaaaccg cgttgctggt gcatctgtta aaacagccgg aagcgacccg ctcaattgtg
    13621 tttgtgcgta agcgtgagcg tgtgcatgag ctggcaaact ggctgcgcga agcaggcatc
    13681 aacaactgct atctcgaagg tgagatggta caaggcaagc gtaacgaagc gattaaacgc
    13741 ttgaccgaag gccgcgtaaa cgtactggtt gctaccgatg ttgccgcgcg cggtatcgac
    13801 attcctgacg tcagccacgt ctttaacttc gatatgccgc gcagtggcga tacttatttg
    13861 caccgtatcg gacgtaccgc gcgcgccggt cgtaaaggca ccgcgatttc gctggtggaa
    13921 gcccatgacc atctgctgct gggcaaagtt ggtcgctata ttgaagagcc aattaaagct
    13981 cgcgtaattg acgagttacg cccgaaaacg cgtgcgccaa gcgagaagca gaccggcaag
    14041 ccatcgaaga aagtactggc aaaacgcgcc gagaagaaaa aagctaaaga gaaagagaag
    14101 ccgcgggtga aaaaacgcca tcgcgacacc aaaaatattg gtaagcgccg taaaccaagc
    14161 ggaacgggcg tgccaccgca aacgacagaa gagtaatctc aatgccgggt ttaagcccgg
    14221 cattgttttt atcccggcaa aaaacactct tcaacacact gaataaaagt gtgcatcgcc
    14281 gggctaaccg cttttccggc atggtgagcg cacattgccg taatggtctg cgactgttcg
    14341 ccaaaaggca attcaattaa ttctccgcac tccaactctt ttaccaccgc aaaacgcggc
    14401 aggtaactga ctccgatatt tgccgcgaca caacgcttga tgctttcgat acttataagc
    14461 tcaatggtgt tttccaccgt gatccgccgc tggcgcagcg tgctctcaaa tatctgccgg
    14521 aagacacatt gtggttcgtt gataataaag ctacaggcgt tatgtcttcc cggctcagta
    14581 aaatcgacat ctgcaatttg cggtgaagcc accagcgcca gcggttgttc acccaactct
    14641 cgtcggttca gagcatcatc attccctacg cgataaaaaa ccccgacatc ggcctcatca
    14701 ttcagtagcg catcacggat cacgtaacag ttcagcgact gcaacgataa acgcactttt
    14761 ggagcgcgtt gccgaaaacg ctgcaacacc tgcggcattc gataagagag caacgtttcg
    14821 cctgaaataa cgcgtagttc cccgtccgga tccgactctt ttttagcagc ctcacgaagc
    14881 gtatccatca ctcgggtaag ttcatagatg tgcggcagca gcttttttcc ttcgcgggta
    14941 aggcacattc gtcgaccaat tttctcaaac aattgtaccg agaactcctg ctcaagctgc
    15001 tgaatatgaa aagtcaccgt cgattgtgtg cagcacaatt tttgcgaagc tcgcaaaaag
    15061 gaaccctctt ccaccacggt tttaagtgta ataaaacggc gcagatccat aaccccaaac
    15121 ctatcgaaaa tatcgaattt agaatgtaaa aacattcatt tttttaaatg ttccgtgtcg
    15181 ggtactgtct accaaaacag aggagataac aagtgacacc gaccctttta agtgcttttt
    15241 ggacttacac cctgattacc gccatgacgc caggaccgaa caatattctc gcccttagct
    15301 ctgctacgtc gcatggattt cgtcaaagta cccgtgtgct ggcagggatg agtctgggat
    15361 ttttgattgt gatgttactg tgtgcgggca tttcattttc actgacagtg attgacccgg
    15421 cagcggtaca ccttttgagt tgggcggggg cggcatatat tgtctggctg gcgtggaaaa
    15481 tcgccaccag cccaacaaag gaagacggac ttcagacaaa accaatcagt ttttgggcca
    15541 gctttgcttt gcagtttgtg aacgtcaaaa tcattttgta cggtgttacg gcactgtcga
    15601 cgtttgttct gccgcaaaca caggcgctaa gctgggtagt tggcgtcagc gttttgctgg
    15661 cgatgattgg gacgtttggc aatgtgtgct gggcgctggc ggggcatctg tttcagcgat
    15721 tgtttcgcca gtatggtcgc cagttaaata tcgtgcttgc cctgttgctg gtctattgcg
    15781 cggtacgcat tttctattaa cgaaaaaaag cggaagaggt cgccctcttc cgcttagtaa
    15841 cttgctactt aagccttaca ggctttcagt aaaggtacga gcgataacgt cgcgctgctg
    15901 ttccggagtc agagagttaa agcgaactgc ataaccggat acacggatgg tcagctgcgg
    15961 atatttttcc ggatgcttaa ctgcatcttc cagagtttca cgacgcagaa cgttaacgtt
    16021 caggtgttga ccaccttcaa cgcgaacttc tggtttcact tctactggaa cttcacggta
    16081 ttcaatgtca cccagtttgc ttactgcaac cacttcgtct tctgcaaaac ctgctttcgc
    16141 aacgatgcaa cgcgcttcgc ctttttcgct gtccagcaac cagaaagagt tcagcagatc
    16201 gtcgttagcg gctttagtaa tctggatacc tgtaatcatg tgatgcctcc ccggcaaaat
    16261 tatttgattt gttcagcctg tcgcggccaa ttggtaaaac cattgttgct tgagtgtata
    16321 tatactcctc aaacaccctt gattctttga tttaaatcaa taaaaaccat atatcaagtg
    16381 tggtcacaaa tggattttat tgttttacat caacttatgc agatgtgaaa ttttaccaat
    16441 ttacattttt
//
LOCUS       DADSYM010000110        16413 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2415
            SAMN08039579-rid11723173.denovo.110, whole genome shotgun sequence.
ACCESSION   DADSYM010000110 DADSYM010000000
VERSION     DADSYM010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039579
            Sequence Read Archive: SRR6362533
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16413)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16413)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980162.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 03:39:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,484
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 4,347
            CDSs (with protein)               :: 4,347
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 43 of 85
            Pseudo Genes (incomplete)         :: 37 of 85
            Pseudo Genes (internal stop)      :: 19 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16413
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039579-rid11723173.denovo.110"
                     /strain="LGE2415"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8302 N 95.9628 W"
                     /collection_date="2015-08-24"
     gene            <1..893
                     /locus_tag="J1Y83_003119"
     CDS             <1..893
                     /locus_tag="J1Y83_003119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBA7631246.1"
                     /translation="NIELRHLRYFVAVAEELHFGRAAARLNISQPPLSQQIQALEQQI
                     GARLLARTNRSVLLTEAGKQFLVDSRQILSMVDDAAARAERLHQGEAGELRIGFTSSA
                     PFIRAVSDTLSLFRRDYPDVHLQTREMNTREQIAPLIEGTLDMGLLRNTALPETLEHA
                     VIVHEPLMAMIPHDHPLANNPSVTLAELAKEPFVFFDPHVGTGLYDDILGLMRRYNLT
                     PVITQEVGEAMTIIGLVSAGLGVSILPASFKRVQLNEMRWVPIAEEDAVSEMWLVWPK
                     HHEQSPAARNFRIHLLNALR"
     gene            1028..2248
                     /gene="mlc"
                     /locus_tag="J1Y83_003120"
                     /gene_synonym="dgsA"
     CDS             1028..2248
                     /gene="mlc"
                     /locus_tag="J1Y83_003120"
                     /gene_synonym="dgsA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor Mlc"
                     /protein_id="HBA7631247.1"
                     /translation="MVAENQPGHIDQIKQTNAGAVYRLIDQLGPVSRIDLSRLAQLAP
                     ASITKIVREMLEAHLVQELEIKEAGNRGRPAVGLVVETEAWHYLSLRISRGEIFLALR
                     DLSSKLVVEESQELALKDDSPLLERIISHIDQFFIRHQKKLERLTSIAITLPGIIDTE
                     NGIVHRMPFYEDVKEMPLGEALEQHTGVPVYIQHDISAWTMAEALFGASRGARDVIQV
                     VIDHNVGAGVITDGHLLHAGSSSLVEIGHTQVDPYGKRCYCGNHGCLETIASVDSILE
                     LAQLRLNQSMSSMLHGQPLTVDSLCQAALRGDLLAKDIITGVGAHVGRILAIMVNLFN
                     PQKILIGSPLSKAADILFPVISDSIRQQALPAYSQHISVESTQFSNQGTMAGAALVKD
                     AMYNGSLLIRLLQG"
     gene            2373..3068
                     /gene="bioD"
                     /locus_tag="J1Y83_003121"
     CDS             2373..3068
                     /gene="bioD"
                     /locus_tag="J1Y83_003121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310326.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent dethiobiotin synthetase BioD"
                     /protein_id="HBA7631248.1"
                     /translation="MLKRFFITGTDTSVGKTVVSRALLQALASQGKTVAGYKPVAKGS
                     KETPEGLRNKDALVLQSVSTIELPYEAVNPIALSEEESSVAHSCPINYTLISNGLANL
                     TDKVDHVVVEGTGGWRSLMNDLRPLSEWVVQEQLPVLMVVGIQEGCINHALLTAQAIA
                     NDGLPLIGWVANRINPGLAHYAEIIDVLGKKLPAPLIGELPYLPRAEQRELGQYIRLA
                     MLRSVLAVDRVTV"
     gene            complement(3021..4313)
                     /gene="clcB"
                     /locus_tag="J1Y83_003122"
     CDS             complement(3021..4313)
                     /gene="clcB"
                     /locus_tag="J1Y83_003122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001296088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated ClC-type chloride channel ClcB"
                     /protein_id="HBA7631249.1"
                     /translation="MHHLHIYPDLRTMFRRLLIATVVGILAAFAVAGFRHAMLLLEWL
                     FLNNDSGSLVNAATNLSPWRRLLTPALGGLAAGLLLVGWQKFTQQRPHAPTDYMEALQ
                     TDGQFDYAASLVKSLASLLVVTSGSAIGREGAMILLAALAASCFAQRFTPRQEWKLWI
                     ACGAAAGMAAAYRAPLAGSLFIAEVLFGTMMLASLGPVIISAVVALLVSNLINHSDAL
                     LYSVQLSVTVQARDYALIISTGVLAGLCGPLLLTLMNACHRGFVSLKLAPPWQLALGG
                     LIVGLLSLFTPAVWGNGYSTVQSFLTAPPLLMIIAGIFLCKLCAVLASSGSGAPGGVF
                     TPTLFIGLAIGMLYGRSLGLWLPDGEEITLLLGLTGMATLLAATTHAPIMSTLMICEM
                     TGEYQLLPGLLIACVIASVISRTLHRDSIYRQHTAKHS"
     gene            complement(4474..5088)
                     /gene="dmsD"
                     /locus_tag="J1Y83_003123"
     CDS             complement(4474..5088)
                     /gene="dmsD"
                     /locus_tag="J1Y83_003123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707489.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tat proofreading chaperone DmsD"
                     /protein_id="HBA7631250.1"
                     /translation="MTHFSQQDNFSVAARVLGALFYYAPESAEAAPLVAVLTRDGWET
                     QWPLPEASLAPLVTAFQTQSEETHAQAWQRLFVGPWALPSPPWGSVWLDRESVLFGDS
                     TLALRQWMREKGIQFEMKQNEPEDHFGSLLLMAAWLAENGRQTECEELLAWHLFPWST
                     RFLDVFIEKAEHPFYRALGELARLTLAQWQSQLLIPVAVKPLFR"
     gene            complement(5131..5316)
                     /locus_tag="J1Y83_003124"
                     /pseudo
     CDS             complement(5131..5316)
                     /locus_tag="J1Y83_003124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310323.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="dimethyl sulfoxide reductase anchor subunit"
     gene            complement(5345..5650)
                     /locus_tag="J1Y83_003125"
     CDS             complement(5345..5650)
                     /locus_tag="J1Y83_003125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1161 domain-containing protein"
                     /protein_id="HBA7631251.1"
                     /translation="MKLSTCCAALLFALASPAVLAAPGSCERIQSDISQRIINNGVPE
                     SSFTLSIVPNDQVDQPDSQVVGHCANDTHKILYTRTTSGNVSAPAQSTQDGAPAEPQ"
     gene            5758..6468
                     /locus_tag="J1Y83_003126"
     CDS             5758..6468
                     /locus_tag="J1Y83_003126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416102.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnfC family lipoprotein"
                     /protein_id="HBA7631252.1"
                     /translation="MKYKLLPCLLAILLTGCDRTEVTLSFTPEMASFSNEFDFDPLRG
                     PVKDFTQTLMDEQGEVTKRVSGTLSEEGCFDSLELLDLENNTLVALVLDANYYRDAET
                     LEKRVRLQGKCQLAELPSAGVSWETDDNGFVIKASSKQMQMEYRYDDQGYPLGKTTTS
                     NDKTLSVSATPSTDPIKKLDYTAVTLLNNQRVGNVKQSCEYDNHANPVDCQLIIVDEG
                     VKPAVERVYTIKNTIDYY"
     gene            complement(6471..7031)
                     /gene="speG"
                     /locus_tag="J1Y83_003127"
     CDS             complement(6471..7031)
                     /gene="speG"
                     /locus_tag="J1Y83_003127"
                     /EC_number="2.3.1.57"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine N1-acetyltransferase"
                     /protein_id="HBA7631253.1"
                     /translation="MPSAHSVKLRPLEREDLRYVHQLDNNASVMRYWFEEPYEAFVEL
                     SDLYDKHIHDQSERRFVVECDGEKAGLVELVEINHVHRRAEFQIIISPEYQGKGLATR
                     AAKLAMDYGFTVLNLYKLYLIVDKENEKAIHIYRKLGFTVEGELMHEFFINGQYRNAI
                     RMCIFQHQYLAEHKTPGQTLLKPTAQ"
     gene            complement(7066..7407)
                     /locus_tag="J1Y83_003128"
     CDS             complement(7066..7407)
                     /locus_tag="J1Y83_003128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1283 family protein"
                     /protein_id="HBA7631254.1"
                     /translation="MKITLSKRIGLLAFLLPCALALSTTVHAETNKLVIESGDSAQSR
                     QRAAMEKEQWNDTRNLRQKVNKRTEKEWDKADAAFDNRDKCEQSANINAYWEPNTLRC
                     LDRRTGRVIIP"
     gene            7542..7868
                     /locus_tag="J1Y83_003129"
     CDS             7542..7868
                     /locus_tag="J1Y83_003129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnfA family protein"
                     /protein_id="HBA7631255.1"
                     /translation="MIKTTLLFFATALCEIIGCFLPWLWLKRNASIWLLLPAGISLAL
                     FVWLLTLHPAASGRVYAAYGGVYVCTALMWLRVVDGVKLTLYDWTGALIALCGMLIIV
                     AGWGRT"
     gene            8074..9288
                     /gene="rspA"
                     /locus_tag="J1Y83_003130"
     CDS             8074..9288
                     /gene="rspA"
                     /locus_tag="J1Y83_003130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001625315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="starvation-sensing protein RspA"
                     /protein_id="HBA7631256.1"
                     /translation="MKIVKAEVFVTCPGRNFVTLKITTEDGITGLGDATLNGRELSVA
                     SYLQDHLCPQLIGRDAHRIEDIWQVFYKGAYWRRGPVTMSAISAVDMALWDIKAKAAN
                     MPLYQLLGGASREGVMVYCHTTGHSIDEALDDYARHQELGFKAIRVQCGIPGMKTTYG
                     MSKGKGLAYEPATKGQWPEEQLWSTEKYLDFMPKLFDAVRNKFGFDEHLLHDMHHRLT
                     PIEAARFGKSIEDYRMFWMEDPTPAENQECFRLIRQHTVTPIAVGEVFNSIWDCKQLI
                     EEQLIDYIRTTLTHAGGITGMRRIADFASLYQVRTGSHGPSDLSPVCMAAALHFDLWV
                     PNFGVQEYMGYSEQMLEVFPHNWTFDNGYMHPGEKPGLGIEFDEKLAAKYPYEPAYLP
                     VARLEDGTLWNW"
     gene            9300..10319
                     /locus_tag="J1Y83_003131"
     CDS             9300..10319
                     /locus_tag="J1Y83_003131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn-dependent oxidoreductase"
                     /protein_id="HBA7631257.1"
                     /translation="MKSILIEKPNQLSIIEREIPTPSAGEVRVKVKLAGICGSDSHIY
                     RGHNPFAKYPRVIGHEFFGVIDAVGEGVESARVGERVAVDPVVSCGHCYPCSIGKPNV
                     CTTLAVLGVHADGGFSEYAVVPAKNAWKIPEAVADQYAVMIEPFTIAANVTGHGQPTE
                     NDTVLVYGAGPIGLTIVQVLKGVYNVKNVIVADRIDERLEKAKESGADWAINNSQTPL
                     GESFAEKGIKPTLIIDAACHPSILKEAVTLASPAARIVLMGFSSEPSEVIQQGITGKE
                     LSIFSSRLNANKFPVVIDWLSKGLIKPEKLITHTFDFQHVADAISLFEQDQKHCCKVL
                     LTFSE"
     gene            10377..10487
                     /locus_tag="J1Y83_003132"
     CDS             10377..10487
                     /locus_tag="J1Y83_003132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MHS family MFS transporter"
                     /protein_id="HBA7631258.1"
                     /translation="MTIEKHERSTKDLVKAAVSGWLGTALEFMDFKSHAC"
     gene            complement(10507..11787)
                     /locus_tag="J1Y83_003133"
     CDS             complement(10507..11787)
                     /locus_tag="J1Y83_003133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001686441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HBA7631259.1"
                     /translation="MKYPTGVENHGGKLRIWFVYKGVRVRENLGVPDTAKNRRVAGEL
                     RSSVCYAIKTGVFDYAKQFPSSRNLEKFGEARQDLTIKELAEKFLALKETEVAKTSLN
                     TYRAVIKNILSIIGEKNLASSINKEKLLEVRKELLTGYQIPKSNYIVTQPGRSAVTVN
                     NYMTNLNAVFQFGVDNGYLADNPFKGISPLKESRTIPDPLSREEFIRLIDACRNQQAK
                     NLWCVSVYTGVRPGELCALGWEDIDLKNGTMMIRRNLAKDRFTVPKTQAGTNRVIHLI
                     KPAIDALRSQMTLTRLSKEHIIDVHLREYGRTEKQKCTFVFQPEVSARVKNYGDHFTV
                     DSIRQMWDAAIKRAGLRHRKSYQSRHTYACWSLTAGANPAFIANQMGHADAQMVFQVY
                     GKWMSENNNAQVALLNTQLIEFAPTMPHNEAMKN"
     gene            complement(11822..12058)
                     /locus_tag="J1Y83_003134"
     CDS             complement(11822..12058)
                     /locus_tag="J1Y83_003134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310314.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase family protein"
                     /protein_id="HBA7631260.1"
                     /translation="MIVSPGKWVSEEQLIALKGIKKGTLKKAREKSFMEGREYKHVAH
                     DGMPWDNSPCFYNLEEIDRWIERQASARPRRHLT"
     gene            complement(12146..14617)
                     /locus_tag="J1Y83_003135"
     CDS             complement(12146..14617)
                     /locus_tag="J1Y83_003135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease"
                     /protein_id="HBA7631261.1"
                     /translation="MSKVFICAAIPDELATREEGAVAVATAIEAGDERRARAKFHWQF
                     LEHYPAAQDCAYKFIVCEDKPGIPRPALDSWDAEYMQENRWDEESASFVPVETESDPM
                     NVTFDKLAPEVQNAVMVKFDTCENITVDMVISAQELLQEDMATFDGHIVEALMKMPEV
                     NAMYPELKLHAIGWVKHKCIPGAKWPEIQAEMRIWKKRREGERKETGKYTSVVDLARA
                     RANQQYTENSTGKISPVVAAIHREYKQTWKTLDDELAYALWPGDVDAGNIDGSIHRWA
                     KKEVIDNDREDWKRISASMRKQPDALRYDRQTIFGLVRERPIDIHKDPVALNKYICEY
                     LTTKGVFENEETDLSTVDVLQSSETQTDAVETEVSDIPKNETAPEAEPSVEREGPFYF
                     LFADKDGEKYGRANKLSGLDKALAAGATEITKEEYFARKNGTYTGLPQNVDTAEDSEQ
                     PEPIKVTADEVNKIMQAANISQPDADKLLAASRGEFVEGISDPNDPKWVKGIQTRDSV
                     NQNQHESERNYQKAEQNSPNALQNEPETKQPEPVAQQEVEKVCTACGQTGGGNCPDCG
                     AVMGDATYQETFDEEYQVEVQEDDPEEMEGAEHPHKENTGGNQHHNSDNETGETADHP
                     IKVNGHHEITSTSRTCDHLMIDLETMGKNPDAPIISIGAIFFDPQTGDMGPEFSKTID
                     LETAGGVIDRDTIKWWLKQSREAQSAIMTDEIPLDDALLQLREFIDENSGEFFVQVWG
                     NGANFDNTILRRSYERQGIPCPWRYYNDRDVRTIVELGKAIDFDARTAIPFEGERHNA
                     LDDARYQAKYVSVIWQKLIPSQADS"
     gene            complement(14711..14902)
                     /locus_tag="J1Y83_003136"
     CDS             complement(14711..14902)
                     /locus_tag="J1Y83_003136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1482 family protein"
                     /protein_id="HBA7631262.1"
                     /translation="MNSAFALVLTVFLVSGVPVDIAVSVHRTMQECMTAATEQKIPGN
                     CYPVDKVIHQDNIEIPVGL"
     gene            complement(14899..15087)
                     /gene="dicB"
                     /locus_tag="J1Y83_003137"
                     /gene_synonym="ftsT"
     CDS             complement(14899..15087)
                     /gene="dicB"
                     /locus_tag="J1Y83_003137"
                     /gene_synonym="ftsT"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division inhibition protein DicB"
                     /protein_id="HBA7631263.1"
                     /translation="MKTLLPNVNTSEGCFEIGVTISNPVFTEDAINKRKQERELLNKI
                     CIVSMLARLRLMPKGCAQ"
     gene            complement(15574..16149)
                     /locus_tag="J1Y83_003138"
     CDS             complement(15574..16149)
                     /locus_tag="J1Y83_003138"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA7631264.1"
                     /translation="MDFDTIMEKAYEEYFEGLAEGEEALSFSEFKQALRIRMCSHNDA
                     EHKYEKQNQTAENFVLEPGETLFKIPVTCPICGFTSEELDDSCNNQETTKYVEDDTEC
                     ARRTIISTSPNSRTNKSHLERVINPLPQTNKKDAGGQKTQWNNGISVKKRRSIKDKQR
                     SANNCKRFIPVINFHVLKRIYWWLRHCLMNP"
     gene            complement(16151..16306)
                     /locus_tag="J1Y83_003139"
     CDS             complement(16151..16306)
                     /locus_tag="J1Y83_003139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1391 domain-containing protein"
                     /protein_id="HBA7631265.1"
                     /translation="MDTIDLGNSESLVCGVFPNQDGTFTAMTYTKSKTFKTENGARRW
                     LERNSGE"
BASE COUNT         4067 a         3905 c         4013 g         4428 t
ORIGIN      
        1 tgaatattga acttcgtcat ctgcgttact ttgtcgctgt tgcagaagag ctgcatttcg
       61 ggcgtgccgc tgcccgcctg aatatttcgc aaccacctct aagtcagcag attcaggcgc
      121 tggagcaaca aattggtgcc cgactgctgg cacggaccaa tcgcagtgta ttgctgacgg
      181 aagcaggaaa acagtttctt gtagatagtc ggcaaatcct gtctatggtg gatgatgctg
      241 ccgctcgcgc tgaaaggctg catcagggcg aagcggggga gttgcgcatt ggttttactt
      301 cgtccgctcc ttttattcgg gcggtgtccg atacgttatc gctgtttcgc cgtgattatc
      361 ccgatgtcca tttacaaacc cgcgaaatga acactcgcga gcaaatcgct ccgctcattg
      421 aaggaacgct ggatatggga ttgctgcgta acacagcgtt accggagacg cttgaacacg
      481 cagtcatcgt ccatgaaccg cttatggcga tgatcccgca cgatcatccc ctggcaaata
      541 acccgagtgt aacgctggct gaactggcga aagaaccctt tgtctttttt gatccgcacg
      601 tcggaacagg gctgtatgac gatattctcg ggctgatgag acgttacaat ttgacgcccg
      661 tcatcactca ggaggtgggt gaggcaatga ccatcatcgg tctggtttcc gctggtctgg
      721 gtgtttcaat tttgcctgcg tcatttaaac gtgttcagct caacgaaatg cgctgggtgc
      781 cgattgctga agaggatgcg gtttctgaaa tgtggttggt ctggccgaaa catcatgaac
      841 aaagtccggc tgcgcgtaac tttcgtattc atctgctgaa tgctctcagg tgagggaaat
      901 ttcagcgaaa aagcccgaaa aatgtgctgt taatcacatg cctaagtaaa aatttgacga
      961 cacgtattga agtgcttcac catagcctac agattatttc ggagcgcgaa aatataggga
     1021 gtatgcggtg gttgctgaaa atcagcctgg gcacattgat caaataaagc agaccaacgc
     1081 gggcgcggtt tatcgcctga ttgatcagct tggtccagtc tcgcgtatcg atctttcccg
     1141 tctggcgcaa ctggctcctg ccagtatcac taaaattgtc cgtgagatgc tcgaagcaca
     1201 cttggtgcaa gagctggaaa tcaaagaagc ggggaaccgt ggccgtccgg cagtggggct
     1261 ggtggttgaa actgaagcct ggcactatct ttctctgcgc attagtcgcg gggagatttt
     1321 ccttgctttg cgcgatctga gcagcaaact ggtagtggag gagtcgcagg aactggcgtt
     1381 aaaagatgac tcgccattgc tggagcgtat catttcccat atcgatcagt tttttatccg
     1441 ccaccagaaa aaacttgagc gtctgacttc gattgccata accttgccgg gaattattga
     1501 tacggaaaat ggtattgtac atcgcatgcc gttctacgag gatgtaaaag agatgccgct
     1561 cggcgaggcg ctggagcagc ataccggcgt accggtttat attcagcatg atatcagcgc
     1621 atggacgatg gctgaggcct tgtttggtgc ctcacgcggg gcgcgcgatg tgattcaggt
     1681 ggttatcgat cacaacgtgg gggcgggcgt cattactgat ggtcatctgc tacacgcagg
     1741 cagcagtagc ctcgtggaaa taggccacac acaggtcgac ccgtatggga aacgctgtta
     1801 ttgcgggaat cacggttgcc tcgaaaccat cgccagtgtg gacagtattc ttgagctggc
     1861 acagctgcgt ctcaatcaat ccatgagctc gatgttacat ggacaaccgt taaccgtgga
     1921 ctcattgtgt caggcggcat tgcgcggcga tctactggca aaagacatca ttaccggggt
     1981 aggcgcgcat gtcggtcgca ttcttgccat catggtgaat ttatttaacc cacaaaaaat
     2041 actgattggc tcaccgttaa gtaaagcggc agatatcctc ttcccagtca tctcggacag
     2101 catccgtcag caggcccttc ctgcgtatag tcagcacatt agcgttgaga gtactcagtt
     2161 ttctaaccag ggcacgatgg caggcgctgc gctggtaaaa gacgcgatgt ataacggttc
     2221 tttgttgatt cgtctgttgc agggttaaca ttttttaact gttctaccaa aatttgcgct
     2281 atctcaattt gggccaggaa agcataactt agactttcaa ggttaattat tttcctggtt
     2341 tatatttgtg aagcataacg gtggagttag tgatgctgaa gcgtttcttt attaccggta
     2401 cagacacttc tgtagggaaa acggtggttt cccgcgcatt gctacaagcg ttagcctccc
     2461 agggaaaaac ggttgcggga tataaacccg tagcgaaggg gagcaaagag acacccgaag
     2521 ggctgcgtaa taaagatgcc ctggtgttgc agagtgtttc aaccatcgaa ctgccttatg
     2581 aagcagttaa tcctatcgcg ttaagcgaag aagaaagtag cgtggcgcac agttgcccaa
     2641 tcaattacac cctcatttca aacggcctgg caaacctgac cgataaagtc gatcatgtcg
     2701 tggtagaagg gactggcggc tggcgcagtc tgatgaatga tttgcgtccg ctctctgaat
     2761 gggtggtgca ggaacaactg ccggtgttga tggttgtcgg tattcaggaa ggttgcatta
     2821 accatgcact gctaacagct caggcgattg ccaacgacgg gctgccgctc attggctggg
     2881 tggctaaccg aatcaaccca ggactggcgc attatgcgga aatcattgat gtgctcggta
     2941 aaaaacttcc ggcaccgctc attggtgaac tgccttatct gccgcgcgcc gaacagcgtg
     3001 aactggggca atacatccgc ttagctatgc ttcgcagtgt gctggcggta gatagagtca
     3061 cggtgtaacg tccgcgaaat taccgacgca attacgcagg caatcaataa accggggagt
     3121 agctgatact ccccggtcat ttcacatatc atcaacgtcg acataatcgg cgcgtgcgtt
     3181 gttgccgcca acagtgtcgc catccctgtc aatccgagta aaagcgtaat ttcttcgcca
     3241 tcaggtaacc ataatcccag gctacgacca tacaacatgc caatggcaag accgataaat
     3301 agcgtcggtg taaagacacc accgggcgct ccagaaccac tactcgccag cacggcacac
     3361 agtttacaga ggaagatccc ggcaatgatc attaacagtg gcggagcggt taaaaaggat
     3421 tgtacggtgc tatagccgtt gccccacact gcaggtgtga acagggaaag cagacccacg
     3481 atcaacccgc ccagtgccag ttgccagggc ggcgcaagct tgagactcac aaatccacga
     3541 tgacaggcgt tcattaacgt taacaacagt ggtccgcaca gacctgccag cacacctgta
     3601 ctgataatca gcgcatagtc acgagcctga accgtcactg agagttgtac actgtagagt
     3661 aacgcgtcgc tatgattaat cagattgcta accagcaacg ccacgacggc ggaaataatc
     3721 accgggccga gagaggccaa catcatagtg ccaaacagca cttcggctat aaataaactg
     3781 ccagcaagcg gggcgcgata ggccgcagcc atccccgccg cagctccaca ggcgatccat
     3841 aatttccact cctggcgtgg cgtaaaacgt tgcgcaaaac aggaggcggc aagggcagct
     3901 aaaagaatca tcgcaccttc gcgaccaatt gcactgccgc tggttactac cagcagagag
     3961 gcaagggatt taaccaggct tgctgcgtaa tcaaactgcc catcggtttg caacgcttcc
     4021 atgtaatcgg tcggcgcatg agggcgttgc tgggtaaatt tctgccagcc caccagcaac
     4081 aaacccgccg ccagtccgcc gagcgccgga gttagcagcc gtcgccaggg ggaaagattt
     4141 gtcgctgcat tgaccagact gcccgagtca ttattgagga acaaccactc cagtagcagc
     4201 atcgcatgac gaaacccggc aacggcaaag gccgcgagaa taccgacgac tgttgcgata
     4261 agcagacggc ggaacatagt gcgcagatca ggatagatgt gtagatgatg catgagcaga
     4321 aatgggcaat gaaacacggg cgactatttt gcggcaaaag aggccagccg caaaagcagt
     4381 gcgtgcttta agagataaaa aaaacgtgac actgtaacct attaattgcc gaattgcgtg
     4441 taatcgtcac catccggcaa taatacggtg atcctatcga aacagcggtt taaccgcgac
     4501 aggaatcaac agttgtgatt gccattgcgc cagcgttaag cgagcgagtt cacccagtgc
     4561 acggtagaaa gggtgttctg ctttttcgat aaaaacgtca agaaaacgtg ttgaccacgg
     4621 aaaaaggtgc catgccagca attcttcgca ttctgtctgg cgaccattct ccgccaacca
     4681 cgcagccatc agcagcagtg aaccaaaatg atcttccggt tcgttttgct tcatttcgaa
     4741 ctgaatgcct ttctcgcgca tccactgacg aagtgccaat gttgaatcgc caaacagcac
     4801 agattcgcga tccagccaga ccgatcccca cggcggagac ggcagtgccc acgggccgac
     4861 aaacaaacgc tgccaggcct gggcgtgagt ctcttcactc tgtgtctgaa aagccgtcac
     4921 cagcggcgct aatgacgctt ctggtagagg ccactgagtt tcccagccgt cacgggtgag
     4981 taccgcaacc agaggtgcgg cttctgcact ctctggagcg taataaaaca gcgcgcccaa
     5041 cacgcgcgcc gcaacagaaa aattatcttg ctgtgaaaaa tgggtcattc ctaacatcct
     5101 gaaatgtgcg ggttgccccg caccaatttg ttaacctgca atagccattc ctaccgtcat
     5161 gtgcaggcca taaaagagca cacgcccgat catctcaccg ccaagaatga ggattaaccc
     5221 cagaacaagt ccggcaacgt gaggttcacg gcgacgaatg agcggacaaa gccagcatcc
     5281 aagacctgcg cataacaata ccacacgcca gacctgtcgt atccggcgtg cagtcgataa
     5341 tgtattactg cggttcggca ggcgcgccat cctgggtaga ctgcgcggga gcagagacgt
     5401 taccgctggt ggtgcgggtg tagagaattt tatgcgtatc attagcgcaa tgcccgacga
     5461 cctgggaatc aggctgatca acctggtcat tgggtacaat acttaacgtg aagctgcttt
     5521 cgggtacgcc attattgata atgcgctgtg atatatcgct ctgtatgcgc tcacaggatc
     5581 ccggcgcggc gagtaccgcg ggtgaggcga gggcgaacag aagcgcggca cagcaggttg
     5641 agagtttcat cataagctcc ttacgcgaag ataacttctt taagcatagc atttaacgtg
     5701 taaagtactg tatttgctac tatgattgag aatcatctct actctcaggt gactgttgtg
     5761 aaatacaaat tactgccatg cttactcgcg atattactca caggatgtga ccgcacagag
     5821 gtaacacttt catttacccc ggagatggcc agtttctcta atgaattcga ttttgatccg
     5881 ctgcgtggtc ccgtaaaaga tttcactcag acattaatgg atgagcaagg tgaagtgacg
     5941 aaacgtgttt cggggacttt gtcggaagaa ggctgttttg attctctcga attactggat
     6001 ctggaaaata ataccttggt tgccctggta ctggacgcca attattaccg tgatgccgag
     6061 acgctggaga aaagagttcg tttgcaggga aaatgccagc tagcagaatt accttctgcc
     6121 ggggtgagtt gggaaaccga tgataatggt ttcgtgatta aagccagcag caaacagatg
     6181 cagatggaat atcgctatga tgatcagggg tatccgctgg gtaaaaccac aacaagtaac
     6241 gataaaacat tatctgtcag cgccacgcca tcaacggacc cgatcaaaaa attagattac
     6301 acagcggtta ctttactgaa taatcaacgg gttggtaatg taaaacagag ctgtgagtat
     6361 gacaatcacg ccaatccggt ggactgtcag ctaatcattg ttgatgaagg agtaaaaccc
     6421 gccgtcgaac gggtttacac catcaaaaat acgatcgatt attattaata ctattgtgcg
     6481 gtcggcttca ggagagtctg acccggtgtt ttgtgctctg ccagatactg atgctggaat
     6541 atacacatgc gaatggcatt acgatattga ccattaataa agaactcatg catcaattca
     6601 ccttcaaccg taaagccaag cttgcggtaa atgtgaatcg ctttttcatt ctctttatca
     6661 acgatcagat acagcttata gagattgaga acggtgaagc catagtccat tgctaatttg
     6721 gcggcacggg ttgccagacc tttcccctga tactccgggg agataattat ctgaaattct
     6781 gcgcggcgat gaacatggtt aatttccacc agctccacca gaccggcttt ttcgccgtca
     6841 cattccacca caaagcgccg ttcgctctga tcgtgaatat gcttatcata cagatcagag
     6901 agttcaacaa aggcttcgta gggttcctca aaccagtaac gcatcacact ggcgttattg
     6961 tcgagttgat gtacatagcg taaatcttca cgctccagcg ggcgtagctt aacactgtgg
     7021 gcgcttggca taacgtgtcc ttacattcct taaatcaata acaggttagg ggataataac
     7081 gcggccagtt cgacggtcca ggcagcgcaa agtatttggc tcccagtagg cattgatgtt
     7141 ggcgctttgc tcacatttat cgcggttatc aaaagcagcg tcggctttat cccactcttt
     7201 ttcagtgcgt ttattcactt tctggcgcag attacgcgtg tcattccatt gctctttttc
     7261 catagcggcg cgctggcggc tttgtgcact gtcgccagac tcaatcacca gtttgttagt
     7321 ttcggcatga acagttgtgc tcaatgccag tgcgcaaggc agcagaaaag cgagcaggcc
     7381 gattcgtttg ctgagagtga ttttcataat tcattccctg tatgaatgat taaagctgat
     7441 tctacaccat ccactgcgaa cgcaaaacgt tcaaggaggg tgtttatatt gatgatatta
     7501 tgtcgcccta taactatacg tgatgtcaat aagtgacaaa gatgattaaa acaacgttac
     7561 tattttttgc tactgcgctg tgtgaaatta ttggatgctt tttgccctgg ctgtggttga
     7621 agcgaaacgc cagtatctgg ctgttgcttc cggcggggat ttcactggcg ctgtttgtct
     7681 ggttgttaac gttgcatcct gcggcgagtg ggcgtgttta cgcggcttat ggtggcgttt
     7741 atgtctgcac ggctttgatg tggctgcgcg ttgtggatgg cgtgaaactg actctttatg
     7801 actggacggg ggcgttaatt gcgctttgcg gcatgttgat cattgttgcg ggctgggggc
     7861 gcacgtagga acataaatcc attttatcaa taagatacga ggaagtgtca gctgacaaaa
     7921 ggtattctat ttcatctttt gtcaaccatt cacggcgcaa atatacgcct ttttttgtga
     7981 tcactccggc tttttttgat cttcatactt gtatggtagt agctcagttg cgtagatttc
     8041 atgcatcacg acaagcgatg caaggaatcg aacatgaaga tcgtaaaggc tgaagttttt
     8101 gttacctgtc cggggcgtaa tttcgtcaca ttaaaaatca ccactgagga cggtattacg
     8161 ggccttgggg atgccaccct aaatggacgt gagctttccg tggcctctta tttgcaggat
     8221 cacctttgtc cgcagcttat tggtcgcgat gcacaccgta tcgaagatat ctggcaggtt
     8281 ttctataaag gtgcttactg gcgtcgcggt ccggttacga tgtcggccat ttcagcggtt
     8341 gatatggcgc tgtgggatat taaagccaaa gctgccaaca tgccgcttta ccagttactc
     8401 ggcggcgcgt ctcgtgaagg ggtgatggtt tattgccata ccaccggtca cagtattgat
     8461 gaagctctgg atgattatgc ccgtcatcag gagctgggat tcaaagccat ccgcgtgcag
     8521 tgcggaatcc ctggtatgaa aaccacctac ggcatgtcga aaggtaaagg tctggcttat
     8581 gaacccgcaa ccaaaggaca gtggccggaa gagcagctgt ggtcgacgga gaaatacctc
     8641 gatttcatgc cgaaattgtt tgacgcggta cgtaacaagt ttggttttga tgaacatctg
     8701 ctgcatgaca tgcaccatcg cttaacgcct attgaagcgg cgcgctttgg taagagcatt
     8761 gaagattatc gcatgttctg gatggaagac ccgacgcctg cggaaaacca ggaatgcttc
     8821 cgtctcattc gccaacatac cgtcacaccc atcgcggtgg gtgaagtctt caacagcatc
     8881 tgggactgca aacaactgat tgaagagcaa ctcatcgatt atatccgcac cacgctgacc
     8941 catgcaggcg gaattaccgg tatgcgccgg attgccgatt ttgcttcgct gtatcaggta
     9001 cgtactggct cacacggtcc ttccgatttg tcaccagtct gcatggctgc ggcgctgcac
     9061 tttgatctgt gggtccccaa tttcggtgtc caggaataca tgggttattc cgaacaaatg
     9121 ctcgaagtct tcccgcacaa ctggactttc gataacggct atatgcatcc gggagaaaaa
     9181 ccgggtcttg gcatcgaatt cgatgaaaag ctggcggcga aatatcccta tgaacctgct
     9241 tatctgccag tcgcacgtct ggaagatggc acgctgtgga actggtaagg agtaaggtaa
     9301 tgaaaagcat tttaattgaa aaaccgaatc aactgtcgat tatcgaacgt gaaataccca
     9361 ccccgtcagc gggtgaagta cgagtaaaag tgaaacttgc cggaatttgt ggttcagata
     9421 gccatattta ccgtgggcat aatccttttg cgaaatatcc gcgcgtcatt ggtcatgaat
     9481 tctttggcgt cattgatgca gtgggtgaag gcgtggaaag cgccagagtc ggtgaacgcg
     9541 ttgctgtcga tccggtggtc agctgtgggc attgctatcc gtgctctata ggtaagccga
     9601 acgtttgtac gacacttgct gtattaggtg tgcacgctga cggtggtttc agtgaatatg
     9661 ctgtggttcc ggcaaaaaat gcgtggaaaa ttcctgaagc tgtggccgat caatatgcgg
     9721 tgatgatcga accttttacc attgcggcta acgttaccgg tcatggtcaa ccgactgaaa
     9781 atgataccgt tctggtttac ggtgccggcc caatcggcct gacgatcgtt caggtattaa
     9841 aaggcgtcta taacgttaaa aatgtgattg ttgccgatcg cattgatgaa cgactggaaa
     9901 aagcgaaaga gagcggggca gactgggcga ttaataacag ccagacaccg cttggcgaga
     9961 gtttcgctga aaaaggcatc aagccgactt taattatcga tgccgcttgt catccttcaa
    10021 tcttgaaaga ggccgtaacg ctggcttctc cagcggcacg tattgtattg atgggcttct
    10081 ccagtgaacc gtctgaagtg attcagcaag gaattaccgg aaaagaactc tctattttct
    10141 cttcacgctt aaatgcaaat aaattcccgg tcgttatcga ctggttaagt aaagggttaa
    10201 ttaaaccaga aaaactaatt acccatacgt ttgatttcca gcatgttgcg gatgccatta
    10261 gtttatttga acaggatcaa aaacattgct gcaaagtctt actcactttt tctgaataat
    10321 accaataacg gcgagtaagt agtacgcatc ttacctcttt tttagagata accattatga
    10381 caatagaaaa acacgaaaga agcactaagg atttggtgaa agcagcagta tcgggatggc
    10441 tgggcactgc gcttgaattt atggatttca agagtcatgc gtgttaacta tttgataaat
    10501 attaaattaa tttttcattg cttcgttatg gggcatggtt ggggcaaact cgattaactg
    10561 tgtatttaac aaagctacct gtgcattatt gttttcagac atccattttc cgtatacctg
    10621 aaataccatt tgcgcatctg catggcccat ctggtttgct ataaatgccg ggttagcacc
    10681 agctgtcagc gaccagcagg cataagtatg tctcgactga tatgattttc gatggcggag
    10741 tccggcacgt tttatcgctg cgtcccacat ctgccttatt gagtcaacgg taaaatggtc
    10801 accataattt tttactctcg ctgacacttc aggttgaaaa acaaaggtgc atttttgttt
    10861 ttctgttctg ccatactctc tgaggtgaac atcaatgata tgctctttgc tcagtctcgt
    10921 taatgtcatc tgactccgga gagcgtcgat tgctggctta ataagatgaa tgacccgatt
    10981 ggttcccgcc tgtgtttttg gtaccgtgaa acggtctttt gctaaatttc tcctgatcat
    11041 cattgttcca tttttcagat ctatgtcctc ccatccaagt gcacacagct caccagggcg
    11101 aacgccagta taaacagaaa cacaccataa attttttgct tgctgatttc tgcacgcatc
    11161 gataagacgg ataaattcct cccgcgaaag aggatccgga atggttcttg attcctttaa
    11221 tggcgagatc cccttaaacg gattatctgc caggtaaccg ttatcaacac caaactggaa
    11281 cacggcgtta agatttgtca tgtaattatt tacagttaca gccgatctcc ctggttgtgt
    11341 aacaatatag ttacttttgg ggatctggta tccagtcagt agctctttac gaacctccag
    11401 taatttttct ttattaatcg atgaggcaag atttttttca ccgattatgc tcaggatatt
    11461 tttgatgacg gcacggtatg tgttgagtga tgttttggcg acttcagttt ctttcagtgc
    11521 cagaaatttt tcagccagtt cttttatggt taaatcttgt cgggcctcac caaatttttc
    11581 cagattgcgt gaggagggaa actgttttgc atagtcgaaa acaccagttt ttattgcgta
    11641 acaaacagag gagcgtagtt cacctgcaac gcgcctgttt tttgctgtgt caggaacccc
    11701 cagattttcc ctgactctta cgcctttata aacaaaccag atacgtaatt tccctccatg
    11761 gttttccacg cctgtcggat atttcatttc aacttctctc attagttagt gtggctttta
    11821 gttaagtaag atgacgtctt ggtctcgctg atgcctggcg ctcaatccag cgatcaattt
    11881 cttccaggtt gtaaaagcat ggactgttat cccatggcat accgtcatga gcgacatgct
    11941 tatattccct tccttccata aacgattttt cccgggcctt ttttaacgta ccttttttta
    12001 ttcctttcag cgcaattaac tgctcttcgg atacccattt gccgggagag acaatcatga
    12061 ttacttcgct catcgatttc tttatctctt acatcagacg agcgccggtt gcagaatacc
    12121 agtcacaacc ggcgacagtt gaacattaag aatcagcctg actcgggatc agtttttgcc
    12181 agataactga aacgtatttt gcctggtaac gggcgtcatc aagtgcatta tggcgctcac
    12241 cttcgaatgg aatagccgtt ctggcatcga agtctatggc tttccccagc tcaacgattg
    12301 tgcgtacatc gcgatcgttg tagtaacgcc acgggcaggg gatcccctgc cgttcgtatg
    12361 aacggcgcaa aatcgtgttg tcgaagttgg ctccatttcc ccagacctga acaaaaaatt
    12421 caccggagtt ttcgtcgata aattcccgca attgtaacag tgcatcatct aacgggattt
    12481 catcggtcat aatggcagat tgcgcttcgc gtgattgctt aagccaccat ttaatggtgt
    12541 cccgatcaat gactccgcca gcagtttcca gatcgatagt cttactaaat tccggtccca
    12601 tatctccggt ttgcggatcg aaaaatattg cacctattga gatgatcggg gcatcaggat
    12661 tttttcccat ggtttcaagg tcgatcatta gatggtcaca cgtcctgctg gtggatgtga
    12721 tttcgtgatg accgttcacc ttaattgggt gatctgccgt ctcgccagtt tcattatcgc
    12781 tattgtgatg ctgattgccg ccagtgttct ccttgtgtgg atgttcagcg ccttccattt
    12841 cctccggatc atcttcctga acttcaacct gatactcttc atcgaatgtt tcttggtatg
    12901 ttgcgtcgcc catcaccgcg ccacaatcag ggcagttgcc gccgccggtc tgaccgcagg
    12961 cggtgcagac tttttccact tcctgttgcg ccactggttc aggctgtttc gtttctggct
    13021 cgttttgtaa cgcatttgga ctgttttgtt ccgctttttg gtagttccgt tccgattcat
    13081 gctggttctg gttcacagaa tcgcgggtct ggatcccctt aacccatttc ggatcattcg
    13141 ggtcactaat cccttcaaca aattcaccac gtgatgcagc aagcaactta tcggcgtcag
    13201 gctggctgat attggctgcc tgcataattt tgtttacttc gtcagcggta acttttatcg
    13261 gctctggttg ttctgaatct tcagcggtat ctacattttg cggtaagccc gtgtatgtgc
    13321 cattttttcg ggcaaaatat tcttcttttg tgatttcagt ggcgccagca gccagtgcct
    13381 tatccagacc agaaagtttg tttgcgcgac cgtatttttc tccgtcctta tctgcgaaga
    13441 ggaaatagaa cggcccctca cgctctacag atggttcagc ttccggcgcg gtttcatttt
    13501 ttgggatatc agatacctca gtttccactg catcagtttg tgtttctgat gactggagaa
    13561 catcaacagt gctcaggtct gtttcttcat tctcaaacac gccctttgtc gtcaggtatt
    13621 cgcagatata tttgttcagt gctacgggat ctttgtgaat gtcgatcgga cgctcacgga
    13681 caaggccaaa aatagtctgg cggtcgtagc gaagggcatc aggctgtttg cgcattgatg
    13741 ccgagatacg cttccagtct tcgcggtcgt tgtcgataac ttcttttttt gcccagcgat
    13801 ggatgctgcc gtcaatgttt ccggcatcca catcaccagg ccagagagcg taggccagtt
    13861 cgtcatccag tgttttccat gtctgcttgt attcgcgatg aatggcagca acgaccgggc
    13921 tgatttttcc tgttgaattt tcagtgtact gttgattggc tctggcgcgg gcgagatcaa
    13981 caacagacgt gtattttccg gtttccttgc gttcaccttc gcgacgtttt ttccagatgc
    14041 gcatctctgc ctgaatttcg ggccatttag caccaggaat acatttatgc ttaacccacc
    14101 caatggcgtg caacttaagc tccggataca tggcgttaac ttctggcatt ttcatcaacg
    14161 cttcaacgat atgtccgtcg aatgttgcca tgtcttcctg caacaattcc tgtgcgctaa
    14221 taaccatatc aacggtgatg ttttcacatg tgtcgaactt aaccatgaca gcgttctgta
    14281 cttcaggggc cagcttgtca aaagtgacgt tcatcggatc ggattcagtc tcaaccggga
    14341 caaaagaagc agactcctca tcccagcggt tttcctgcat atattcagca tcccatgaat
    14401 cgagggcagg gcggggtatg ccaggtttat cctcgcagac aataaattta taagcgcagt
    14461 cctgagcagc cggataatgt tccaggaatt gccagtgaaa ttttgctcga gcacggcgtt
    14521 cgtcgccagc ttcaatggct gtggctacag ccacagcgcc ttcttccctt gttgccagtt
    14581 cgtcaggaat agcggcgcaa ataaagactt tactcatttt gttttaacct catgacagat
    14641 ttaaggatga acaaatccct gccattgctg gcatataaga atgaaaccgg atatttatta
    14701 cggaactgtt ttaaagacct accgggattt cgatattatc ctggtgaata actttatcga
    14761 ccgggtaaca gttaccggga attttctgtt cggttgctgc agtcatacac tcctgcattg
    14821 tcctgtgaac actgactgca atatcaactg gcactccgga aacaagaaaa actgtcagaa
    14881 caagcgcaaa tgctgaattc attgtgcaca tccttttggc atcagacgta aacgagccag
    14941 cattgaaaca atgcatattt tatttaatag ctcccgttct tgttttctct tgttaatggc
    15001 atcttcagta aatacagggt tactgatagt gacaccaatt tcaaaacaac cttcagacgt
    15061 attaacgttt ggtaataacg ttttcattat cgcgtcctca acaatgaatt ttgtgatgca
    15121 gtgcctggtg cctccaggtg acgttaacca gttaacaatt aacgtcggat acagagaatc
    15181 cacccataac actgtttttg gttttaactg ttccgcgtgc gcttagccgc attcaccgca
    15241 tcacaaaatt cactttaaaa acggcggcag agcagtcacg gagtaaaact gataccgcca
    15301 aacgtcacca gaaaattgat aacagagggc gttgcagcgg ggttgtcact taagcgtatg
    15361 gtcaacctga caactcggtg tcctcaacgg ggaaggaata accccgccat acttaccgcc
    15421 gcgccatttt gcgggttgcc acaaccggaa gcgcacggtc gaattaaatt taacgacacc
    15481 gtacagtgag acgaacttcg ccgtgcgctt tcgtgttgtg tgcctgcttt taaccacgtc
    15541 aggcgaggtg gtatccttaa aatcaccaca gttttaagga ttcattaagc aatgtcgcaa
    15601 ccaccaataa atccgcttaa gaacatgaaa attgattact ggtataaagc gcttacagtt
    15661 gttggcgctg cgttgtttgt ctttaatgga acgtcttttt ttgacagata tcccgttgtt
    15721 ccattgggtt ttttgtcctc cggcatcttt tttattggtt tgggggagtg gattaatcac
    15781 cctctcaagg tgagatttat tggtcctgga gtttggactc gtggatataa tcgttcttcg
    15841 tgcgcactcg gtatcatctt cgacatactt ggttgtttcc tgattgttac aggagtcgtc
    15901 aagttcttct gatgtaaaac cgcaaatggg gcacgtaacg ggaattttga aaagcgtttc
    15961 tccgggttcc agaacaaaat tttctgcggt ctgattttgc ttctcatatt tgtgctccgc
    16021 gtcattgtga gagcacattc ttattctgag tgcctgttta aactcactga agctgagagc
    16081 ttcttcgcct tcggcaaggc cttcgaagta ttcttcgtaa gccttttcca tgattgtgtc
    16141 gaaatccata tcactcacct gagtttcttt ccagccagcg acgggcacca ttttcggttt
    16201 taaacgtttt gcttttggta tacgtcatcg cggtgaacgt gccgtcctgg ttggggaaca
    16261 cgccacatac cagagattcg ctgttgccaa gatcgatagt atccatgctg acctcatttc
    16321 cccttaacgc cggggtcgcg gaactgtttg ctgagaacac cgtgcggtgt cttgatggaa
    16381 agtaatttag aataacctaa catgagaggc aag
//
LOCUS       DADSYM010000111        16182 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2415
            SAMN08039579-rid11723173.denovo.111, whole genome shotgun sequence.
ACCESSION   DADSYM010000111 DADSYM010000000
VERSION     DADSYM010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039579
            Sequence Read Archive: SRR6362533
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16182)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16182)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980162.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 03:39:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,484
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 4,347
            CDSs (with protein)               :: 4,347
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 43 of 85
            Pseudo Genes (incomplete)         :: 37 of 85
            Pseudo Genes (internal stop)      :: 19 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16182
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039579-rid11723173.denovo.111"
                     /strain="LGE2415"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8302 N 95.9628 W"
                     /collection_date="2015-08-24"
     gene            <1..1364
                     /gene="dhaR"
                     /locus_tag="J1Y83_003140"
     CDS             <1..1364
                     /gene="dhaR"
                     /locus_tag="J1Y83_003140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000351361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PTS-dependent dihydroxyacetone kinase operon
                     transcriptional regulator DhaR"
                     /protein_id="HBA7631266.1"
                     /translation="TSYSCATEIANYIYIENEQKLINKVLCQHNAVIECMDDGFICWN
                     SHSLITMVNSQAQTLLNIDKESLIGQNIRKGFVFPPILNEAITQRNKLSQKQIVLECR
                     GEFIELMVTLRPLSDGSFLLFLHPLDKIRKIAQQQISTNANFTFDSLHAASGGMKQVL
                     LIARRAIKSISPILINGEEGVGKLSLAMAIHNESEQRDGPFISVDCQMLSPENILHEL
                     LGSDVGPSPSKFELAHNGTLYLDKVEYLSGEVQSVLLKVLKTGLVTRSDSHRLIPVRF
                     RLITCTSSSLREYVQQGAFSRQLYYEISMNEIEIPPLRKRREDLKQMIDDIIDKYQER
                     TRKKMTITPDANSVLLEYRWPGNISEFKNRMEKVFINCNRLVLGLENIPLDIRQNNSS
                     GDDDIPHLTSLAELEMQAIEHTCRVCEWNLTKAAEVLKIGRTTLWRKLKIYNLYPNVE
                     HAD"
     gene            1651..3021
                     /gene="ibeA"
                     /locus_tag="J1Y83_003141"
     CDS             1651..3021
                     /gene="ibeA"
                     /locus_tag="J1Y83_003141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000397940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative intracellular survival FAD-dependent
                     oxidoreductase IbeA"
                     /protein_id="HBA7631267.1"
                     /translation="MEFYLEPARNIPVLATTEVLVVGGGPSGIAAAMSAAREGAATML
                     IERFGCFGGMMTTAGVESIAWWRHENTVESGGLAREIEETAKSMGASSPEPQSNSQAI
                     NAERFKLVADAMLEQAGVRRVLHITAVDVIKQGNNLLGVITESKSGRQAILANVIIDC
                     TGDADIAWFAGAPFTKREREELMCMTTVFSCANINKNAFMQNIKSTEPKYGDWGADEE
                     NKNWSYDVHESCRDMFSPYLGKVFAKGKSAGIIPKDVTLGGSWSTVTEYGDANYLNVV
                     SIPAVDCTDVFDLTRAEIEGRKQAMQAIEALRQFQPGFEQAQLKNFGMTVGTRESRHI
                     LGRTQLTENDICNEGRHADSIGVFPEFIDGNGHLKLPLEANYFQIPYGVMIPQQVENL
                     LVCGRAIDADNFAYATIRNMGCCIVTGEGAGTAAAIAIKNNTTVSQVDIQTVQERLQQ
                     NGVKVF"
     gene            3036..4439
                     /gene="nhaC"
                     /locus_tag="J1Y83_003142"
     CDS             3036..4439
                     /gene="nhaC"
                     /locus_tag="J1Y83_003142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001208425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HBA7631268.1"
                     /translation="MREKPSFYVALTPIIFMMIVLVVGIGVMGWPASVCLLISAAFSC
                     IIAMAKLKYTWDEIQGFIIDKISAVMAPVLIVIFVGFMVATWSYAGTLPMLVYYGMLL
                     VAPAWLYAIAFFLNAVLSYVSGASWGSVASIGVALMGIGSGLHADLPILAAAIVTGAY
                     FGDKLSPLSDTTNLTSAVTKTKLYDLIKYLLWTTIPSTIISLLFFTWLGMIHTSTGSI
                     SPESMQMLVQLKELYKFGLLPLLPMLIVLCFTFLRLPTVPALLSSAFVAVLVGWLYQG
                     FPLAEGIKATMSGFRLTMITDSTTLLPSVKELLEQGGLNNQGGFLSFIICAMMFAGIL
                     TGTGMMDVTLQSAANKIKSAFGAILASGVLAIIINLLTGSDGLNKIVISELMMKKFED
                     LNLSPLVLARTLEDFGTMSAPIIPWSAAGLYMATTLGVPTFSYLPYCVFCFCSMIFAL
                     IYASTGFRLLRFNEAKA"
     gene            complement(4526..5437)
                     /gene="hypT"
                     /locus_tag="J1Y83_003143"
                     /gene_synonym="qseD"
     CDS             complement(4526..5437)
                     /gene="hypT"
                     /locus_tag="J1Y83_003143"
                     /gene_synonym="qseD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypochlorite stress DNA-binding transcriptional
                     regulator HypT"
                     /protein_id="HBA7631269.1"
                     /translation="MDDCGAVLHNIETKWLYDFLTLEKCRNFSQAAVSRNVSQPAFSR
                     RIRALEQAIGVELFNRQVTPLQLSEQGKIFHSQIRHLLQQLESNLAELRGGSDYAQRK
                     IKIAAAHSLSLGLLPSIISQMPPLFTWAIEAIDVDEAVDKLREGQSDCIFSFHDEDLL
                     EAPFDHIRLFESQLFPVCASDEHGEALFNLAQPHFPLLNYSRNSYMGRLINRTLTRHS
                     ELSFSTFFVSSMSELLKQIALDGCGIAWLPEYAIQQEIRSGQLVVLNRDELVIPIQAY
                     AYRMNTRMNPVAERFWRELRELEIVLS"
     gene            complement(5502..6674)
                     /gene="iadA"
                     /locus_tag="J1Y83_003144"
     CDS             complement(5502..6674)
                     /gene="iadA"
                     /locus_tag="J1Y83_003144"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HBA7631270.1"
                     /translation="MIDYSAAGFTLLQGAHLYAPEDRGICDVLIANGKIIAVASNIPS
                     DIVPDCTVVDLSGQILCPGFIDQHVHLIGGGGEAGPTTRTPEVALSRLTEAGVTSVVG
                     LLGTDSISRHPESLLAKTRALNEEGISAWMLTGAYHVPSRTITGSVEKDVAIIDRVIG
                     VKCAISDHRSAAPDVYHLANMAAESRVGGLLGGKPGVTVFHMGDSKKALQPVYDLLEN
                     CDVPISKLLPTHVNRNVPLFEQALEFARKGGTIDITSSIDEPVAPAEGIARAVQAGIP
                     LARVTLSSDGNGSQPFFDDEGNLTHIGVAGFETLLETVQVLVKDYDFSISDALRPLTS
                     SVAGFLNLTGKGEILPGNDADLLVMTPELRIEQVYARGKLMVKDGKACVKGTFETA"
     gene            complement(6687..7148)
                     /locus_tag="J1Y83_003145"
     CDS             complement(6687..7148)
                     /locus_tag="J1Y83_003145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA7631271.1"
                     /translation="MTTQVRKNVMDMFIDGARRGFTIATTNLLPNVVMAFVIIQALKI
                     TGLLDWVGHICEPVMALWGLPGEAATVLLAALMSMGGAVGVAASLATAGALTGHDVTV
                     LLPAMYLMGNPVQNVGRCLGTAEVNAKYYPHIITVCVINALLSIWVMQLIV"
     gene            complement(7145..7828)
                     /locus_tag="J1Y83_003146"
     CDS             complement(7145..7828)
                     /locus_tag="J1Y83_003146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418750.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjiH family protein"
                     /protein_id="HBA7631272.1"
                     /translation="MTQQGDAVAGELATEKVGVKGYLAFFLTIIFFSGVFSGTDSWWR
                     VFDFSVLNGSFGQLPGANGATTSFRGAGGTGAKDGFLFALELAPSVILSLGIISITDG
                     LGGLRAAQQLMTPVLKPLLGIPGICSLALIANLQNTDAAAGMTKELAQEGEITERDKV
                     IFAAYQTSGSAIITNYFSSGVAVFAFLGTSVIVPLAVILVFKFVGANILRVWLNFEER
                     RNPTQGAQA"
     gene            8078..8632
                     /gene="kptA"
                     /locus_tag="J1Y83_003147"
     CDS             8078..8632
                     /gene="kptA"
                     /locus_tag="J1Y83_003147"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418751.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2'-phosphotransferase"
                     /protein_id="HBA7631273.1"
                     /translation="MAKYNDKELAETSKFLSFVLRHKPEAIGIVLDREGWADIDKLIL
                     CAQKAGKRLTRALLDTVVATSDKKRFSYSSDGTCIRAVQGHSTSQVVIAFIEKTPPQF
                     LYHGTASRFLEAIKKQGLIAGERHYVHLSADEATARKVGARHGSPVILTVKAQEMAKR
                     GLPFWQAENGVWLTSTVAVEFLEL"
     gene            complement(8645..9823)
                     /locus_tag="J1Y83_003148"
     CDS             complement(8645..9823)
                     /locus_tag="J1Y83_003148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBA7631274.1"
                     /translation="MPSSTHPVERFSFSTALFGMLVLTLGMGLGRFLYTPMLPVMMAE
                     GSFSFSQLSWIASGNYAGYLAGSLLFSFGAFHQPSRLRPFLLASALASGLLILAMAWL
                     LPFILVLLIRVLAGVASAGMLIFGSTLIMQHTRHPFVLAALFSGVGIGIALGNEYVLA
                     GLHFDLSSQTLWQGAGALSGMMLIALTLLMPSKKHAITPMPLAKTEQQIMSWWLLAIL
                     YGLAGFGYIIVATYLPLMAKDAGSPLLTAHLWTLVGLSIVPGCFGWLWAAKRWGALPC
                     LTANLLVQAISVLLTLASDSPLLLIISSIGFGSTFMGTTSLVMTIARQLSVPGNLNLL
                     GFVTLIYGIGQILGPALTSMLGNGTSTLAGATLCGAAALFIAALISTVQLFKLQVVTS
                     "
     gene            complement(9891..10751)
                     /locus_tag="J1Y83_003149"
     CDS             complement(9891..10751)
                     /locus_tag="J1Y83_003149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418753.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjiK family protein"
                     /protein_id="HBA7631275.1"
                     /translation="MTKSISLSKRIFVIVILFVIVAVCTFFVQSCARKSNHAASFQNY
                     HATIDGKEIAGITNNISSLTWSAQSNTLFSTINKPAAIVEMTTNGDLIRTIPLDFVKD
                     LETIEYIGDNQFVISDERDYAIYVISLTPNSEVKILKKIKIPLQESPTNCGFEGLAYS
                     RQDHTFWFFKEKNPIEVYKVNGLLSSNELHISKDKALQRQFTLDDVSGAEFNQQKNTL
                     LVLSHESRALQEVTLVGEVIGEMSLTKGSRGLSHNIKQAEGVAMDASGNLYIVSEPNR
                     FYRFTPQSSH"
     gene            complement(10816..11073)
                     /locus_tag="J1Y83_003150"
     CDS             complement(10816..11073)
                     /locus_tag="J1Y83_003150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3343 domain-containing protein"
                     /protein_id="HBA7631276.1"
                     /translation="MKEYLFLFHSTVGVIQTRKALQAAGMTFRVSDIPRDLRGGCGLC
                     IWLTCPPGEEIQWVIPGHTESVYCQQDGGWRCIAHYGISPR"
     gene            complement(11070..11837)
                     /locus_tag="J1Y83_003151"
     CDS             complement(11070..11837)
                     /locus_tag="J1Y83_003151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001298028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA7631277.1"
                     /translation="MTYSIGIDSGSTATKGILLADGVITRRFLVPTPFRPATAITEAW
                     ETLREGLETTPFLTLTGYGRQLVDFADKQVTEISCHGLGARFLEPATRAVIDIGGQDS
                     KVIQLDDDGNLCDFLMNDKCAAGTGRFLEVISRTLGTSVEQLDSITENVIPHAITSMC
                     TVFAESEVISLRSAGVAPEAILAGVINAMARRSANFIARLSCEAPILFTGGVSHCQRF
                     THMLESHLGMPVQTHPDAQFAGAIGAAVIGQRQRKRA"
     gene            complement(11847..12998)
                     /locus_tag="J1Y83_003152"
     CDS             complement(11847..12998)
                     /locus_tag="J1Y83_003152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418755.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacyl-CoA dehydratase subunit D"
                     /protein_id="HBA7631278.1"
                     /translation="MSLVTDLPAIFDQFSEARQKGFLTVMDLKERGIPLVGTYCTFMP
                     QEIPMAAGAVVVSLCSTSDETIEEAEKDLPRNLCPLIKSSYGFGKTDKCPYFYFSDLV
                     VGETTCDGKKKMYEYMAEFKPVHVMQLPNSVKDDASRALWKAEMLRLQKTVEERFGHE
                     ISEDALRDAIALKNRERRALANFYHLGQLNPPALSGSDILKVVYGATFRFDKEALINE
                     LDAMTARVRQQWEEGQRLGPRPRILITGCPIGGAAEKVVRAIEENGGWVVGYENCTGA
                     KATEQCVAETGDVYDALADKYLAIGCSCVSPNDQRLQMLSQMVEEYQVDGVVDVILQA
                     CHTYAVESLAIKRHVRQQHNIPYIAIETDYSTSDVGQLSTRVAAFIEML"
     gene            complement(13114..14394)
                     /locus_tag="J1Y83_003153"
     CDS             complement(13114..14394)
                     /locus_tag="J1Y83_003153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001741264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF445 domain-containing protein"
                     /protein_id="HBA7631279.1"
                     /translation="MNKLIELRRAKMLALSLLLIAAATFVVTLFLPPNFWVSGVKAIA
                     EAAMVGALADWFAVVALFRRVPIPIISRHTAIIPRNKDRIGENLGQFVQEKFLDTQSL
                     VALIRRHEPALLIGNWFSQPENARRVGQHLLQIMSGFLELTDDARIQRLLKRAVHRAI
                     DKVDLSGTSALILESMTRNDRHQVLLDTLIAQLIALLQRDKSRKFIAQQIVRWLESEH
                     PLKAKILPTEWLGEHSAELVSDAVNSLLDDISRDRAHQIRHAFDRATFALIDKLKNDP
                     EMAARADAVKSYLKEDEAFNRYLSELWGDLREWLKADINSEDSRVKERIARAGQWFGE
                     TLIADDALRASLNGHLEQAAHRVAPEFSAFLTRHISDTVKSWDARDMSRQIELNIGKD
                     LQFIRVNGTLVGGCIGLILYLLSQLPALFPLGNL"
     gene            14579..15499
                     /locus_tag="J1Y83_003154"
     CDS             14579..15499
                     /locus_tag="J1Y83_003154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001678496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HBA7631280.1"
                     /translation="MTNFTTSTPHDALFKSFLTHSDTARDFMEIHLPKDLRELCDLDS
                     LKLESASFVDEKLRALHSDILWSVKTREGDGYIYVVIEHQSREDIHMAFRLMRYSMAV
                     MQRHIEHDKRRQLPLVIPMLFYHGSRSPYPWSLCWLDEFADPTTARKLYTAAFPLVDI
                     TVVPDDEIVQHRRVALLELIQKHIRQRDLMGLIDQLAVLLVTGCANDSQITALLNYIL
                     LAGDEARFKEFISELTRRMPQHRERIMTIAERIHNDGYIKGEQRILRLFLQNGVDPEW
                     IQKITGLSAEQIQALEQPLPDREHDSWLES"
     gene            complement(15629..15799)
                     /locus_tag="J1Y83_003155"
     CDS             complement(15629..15799)
                     /locus_tag="J1Y83_003155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000199357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBA7631281.1"
                     /translation="MTRYQHLATLLAERIEQGLYRHGEKLPSVRSLSQEHGVSTVQQA
                     YQTLETMNLITP"
     gene            15977..16141
                     /locus_tag="J1Y83_003156"
                     /pseudo
     CDS             15977..16141
                     /locus_tag="J1Y83_003156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008786105.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1127 domain-containing protein"
BASE COUNT         4255 a         3850 c         4047 g         4030 t
ORIGIN      
        1 aaacatcata ttcatgtgca acagaaatcg ctaattatat ctatattgag aatgaacaaa
       61 agttaataaa caaagtactt tgccagcata atgctgtgat tgaatgtatg gatgacggtt
      121 ttatttgttg gaatagtcat tctttaatta cgatggttaa ttctcaagca caaacactac
      181 tgaatattga taaagaaagt ttaattggtc agaacatccg aaaaggattc gtatttccgc
      241 cgattttgaa tgaggcaatt acacaacgaa acaaactatc gcaaaagcaa attgtcctcg
      301 aatgccgtgg cgaatttatt gaacttatgg ttacccttcg cccgttaagc gatggttcgt
      361 ttttgctttt tcttcatcca ttagacaaaa tcaggaaaat agcccaacag caaataagca
      421 ctaatgcaaa ttttaccttt gacagtttac atgcggcttc aggtggtatg aagcaggtat
      481 tacttatcgc tcgccgggca attaaatcca tctctccgat tttgatcaat ggcgaagaag
      541 gtgtgggaaa attgagtttg gcgatggcaa tacataatga gagcgagcaa cgtgatgggc
      601 catttatttc tgtagattgt cagatgctat caccagaaaa tatcttacac gaacttcttg
      661 gctctgatgt tggcccttcg ccatcgaaat ttgaactggc tcataatggc accttatatc
      721 tggataaagt cgaatatcta tcaggggaag ttcagagtgt tttattgaaa gtattgaaaa
      781 cggggcttgt tactcgctca gacagccatc gtttgatccc cgtacgcttt cgcctgatta
      841 catgtaccag tagttcttta cgtgagtacg tgcaacaagg ggcttttagc cgacagctat
      901 attatgagat ctccatgaat gaaattgaaa ttccgccatt gcgcaaacgt cgtgaagatc
      961 tcaagcaaat gattgacgat attattgata agtatcagga gcgcactcga aaaaaaatga
     1021 caatcacgcc tgacgcaaat tcagttctgc ttgagtaccg ttggcctggg aacatctccg
     1081 agttcaaaaa tcgaatggag aaggtattta ttaactgcaa tcgacttgtc ctcgggttag
     1141 agaatattcc tctggatatc cgacaaaata acagtagtgg cgacgatgat atccctcatc
     1201 ttacttcact ggcagaattg gagatgcaag ctattgagca tacatgtcgt gtctgcgaat
     1261 ggaatctaac taaagcagct gaagtattaa aaattggtcg tacaacatta tggcgcaaac
     1321 ttaaaatcta taatctctat ccaaatgttg agcatgcaga ttgatgcttt tcaaattgaa
     1381 ataattttaa tgtcgcagtg tttcattttg gtgcagtgct ttaatggtat tgaaatattg
     1441 tgatgtgctc cataaatgcc aatgtggtta ttagccaggg agacggaaaa tgtgtttaca
     1501 ctcctgaaat aataaaaaac aggcaaagag agatgatctc cttgtatgtc tgtattagca
     1561 tgatgttgct tgttaataat taaaaatatc aacgcgctta tataagcgcg ggggattgtt
     1621 ttactcaatt attgaatacg gagataaagt atggaatttt atctggaacc cgctcgtaat
     1681 atacctgtgc tggcaaccac ggaagtgtta gttgttggtg gtggtccatc gggtattgcc
     1741 gcagcaatga gtgccgctcg tgaaggcgca gctactatgc tgattgaacg tttcggttgt
     1801 tttggcggaa tgatgacaac ggctggcgtc gagtcaattg cctggtggcg tcatgaaaat
     1861 acggtagagt caggtggact ggcacgcgaa atagaagaaa cggcaaaatc aatgggggcg
     1921 tccagccctg agccgcaatc gaatagtcag gctattaacg ccgagcgttt caaactggtt
     1981 gcggatgcaa tgcttgaaca ggcaggtgtg cgccgcgtac tacacattac cgccgttgat
     2041 gttatcaagc agggcaataa tttactcggc gtaataacag agagtaaatc tggtcgtcag
     2101 gctattttgg caaatgtcat tattgactgt actggcgatg ctgatattgc atggtttgcc
     2161 ggagcaccat ttactaagcg tgaacgcgaa gagctaatgt gtatgacaac cgtttttagt
     2221 tgcgcaaata taaataaaaa tgcgttcatg caaaatatta agagcacgga acctaaatat
     2281 ggagactggg gggcggatga agaaaataaa aactggtctt atgatgttca tgaatcttgt
     2341 cgcgatatgt ttagccctta tctgggtaaa gtctttgcga aaggaaagtc ggcaggaatt
     2401 attccaaaag atgtgacgtt aggcggttcc tggagtacgg tcaccgagta tggtgatgcg
     2461 aattatttga atgttgttag catccccgcc gttgattgta cagatgtttt tgacctgacg
     2521 cgtgccgaaa tagagggccg caaacaagcc atgcaggcga tagaagcgtt gcgtcaattc
     2581 cagccaggat ttgaacaggc acaattaaaa aacttcggta tgacggtggg aacaagggaa
     2641 tcaagacata ttctcgggcg aacacaatta actgaaaatg atatttgtaa tgagggacgt
     2701 catgcggatt caataggggt attccctgag tttatagatg gaaatggtca tcttaaatta
     2761 cctcttgaag cgaactattt tcaaatccct tatggcgtaa tgattccgca gcaagttgaa
     2821 aacctgttgg tttgcggacg ggcaatcgat gcagataatt tcgcctacgc gacaatccgt
     2881 aatatggggt gttgtattgt cactggagaa ggtgcaggga ctgccgctgc tattgccatt
     2941 aaaaataaca ctaccgtttc acaggtagat attcagacgg tacaggaacg cttacagcaa
     3001 aatggcgtaa aagtctttta acagggaata aagttatgag agaaaaaccc agtttttatg
     3061 tcgcgcttac accgatcatt tttatgatga tcgtgttggt tgttggcatc ggtgttatgg
     3121 gctggcctgc cagcgtctgt ttattaattt cagcggcgtt ttcctgcatt attgcaatgg
     3181 ctaagctgaa atatacctgg gatgaaatcc aggggtttat tatcgataag atttcggcgg
     3241 tgatggcacc agtacttatc gttattttcg ttggctttat ggtcgcgaca tggagctatg
     3301 caggtacact gccgatgctg gtttattacg gtatgttact ggttgcgccc gcatggcttt
     3361 atgccatagc gttttttctg aacgcagtat tgtcctatgt ttccggggca tcatggggat
     3421 ctgtcgcttc tattggtgtt gcattaatgg gcatcggttc cggcttacat gctgatcttc
     3481 caattctggc ggcagccatt gttaccgggg cttattttgg cgacaaactt tcaccgttaa
     3541 gcgataccac taacctgact tcagcagtaa ctaaaactaa actttatgat ctgattaaat
     3601 atctactctg gacgaccatc ccatcaacga ttattagcct gctgtttttt acctggctgg
     3661 gaatgattca tacttctacg ggctctattt caccagaaag catgcagatg ctggtacagc
     3721 tcaaagagtt atataagttt ggtctgttac cgctactgcc tatgcttatt gttctgtgct
     3781 ttaccttttt gcgtttgcct acagtaccgg cattattaag cagtgcgttc gtggccgtat
     3841 tggttggatg gctgtatcaa ggtttcccgc tggccgaagg tattaaggcc acaatgagtg
     3901 gtttccgtct gacgatgatc actgattcaa cgacgctgtt accttctgtg aaagaactgc
     3961 ttgaacaagg cggcctgaat aatcagggcg gattcctgtc atttattatc tgcgccatga
     4021 tgtttgctgg catcctgacg ggaacaggaa tgatggatgt cacattacaa agtgcggcaa
     4081 acaaaataaa aagtgccttt ggcgctattc tggcaagcgg tgttttagcg atcatcatta
     4141 acttacttac cggttctgat ggtttgaata agattgttat atctgaattg atgatgaaga
     4201 aattcgaaga tcttaattta tcaccgttag tcttagcccg aacgctggaa gattttggca
     4261 ccatgagcgc gccaattatt ccgtggtcag ctgccggatt gtatatggcg acaacactag
     4321 gcgtgccgac gtttagttac ttgccgtact gtgtattctg tttctgctcg atgatatttg
     4381 cgttgatcta tgcttctaca ggtttccgtt tactgcgctt caacgaagca aaggcctgag
     4441 ttattcaaga taataaatgc gtttgccgga tggattaatc tccatccggc gttaatgtca
     4501 aaattccatg ttttgcagaa taatgtcagc taagcacaat ctccagctcg cgcagttcac
     4561 gccagaagcg ttcggcaact ggattcatgc gggtattcat ccggtatgca taggcctgaa
     4621 tcgggattac cagttcgtcc cgattcagta caacgagctg cccgctgcga atttcttgtt
     4681 gtatggcgta ctccggtagc caggcaattc cacatccgtc gagggcaatc tgctttaaaa
     4741 gctcgctcat cgaagagaca aaaaaggtgc tgaaactcaa ctcactgtgg cgcgtcagag
     4801 tgcgattaat caatcgtccc atgtaggagt tgcggctgta attcagtaac ggaaagtgtg
     4861 gctgagcgag gttaaaaagt gcttctccgt gttcgtcact ggcgcagacg gggaacaatt
     4921 gagattcaaa taagcggatg tggtcaaacg gcgcttccag caaatcttcg tcgtgaaagg
     4981 aaaaaataca gtcactttgc ccttcacgca gtttatcgac cgcttcatcg acatcaatgg
     5041 cttcaattgc ccaggtaaag agcggcggca tctggctgat aatggacggt aacagaccga
     5101 gggaaagaga gtgtgcagcg gctatcttga ttttacgttg cgcgtaatcg ctaccgccac
     5161 gcagctctgc cagattgctc tctaactgtt gcaacagatg gcggatctgc gaatggaaga
     5221 ttttcccttg ttctgagagt tgcagcggcg ttacctggcg gttaaacaat tcaacaccaa
     5281 tcgcctgttc cagcgcacgg atgcggcggc tgaatgctgg ttgcgagacg ttgcgactga
     5341 ctgccgcctg ggaaaaattg cgacattttt ccagggtcag aaaatcataa agccatttgg
     5401 tttcaatgtt atgcaaaacc gcaccacagt catccattac gttatactct gtcagccgcc
     5461 cttgcgggca ttctacgtcc attcgggcgg ctgacaaccg tttatgccgt ttcaaacgtt
     5521 cctttcacgc aggctttgcc gtctttgacc atcagtttgc cgcgagcgta tacctgctca
     5581 atgcgcaatt caggcgtcat gaccagtaag tctgcgtcat tgcctggcag aatttcgcct
     5641 ttcccggtca ggttaagaaa accggctacg ctactggtga gcgggcgcag ggcatcgctg
     5701 atactgaaat catagtcttt gaccagcacc tgcacggttt ccagcaatgt ttcaaaacct
     5761 gcaacaccga tatgggttaa atttccttcg tcatcgaaga acggctggct accgttgccg
     5821 tcggagctga gagtgacgcg tgccagcgga atacccgcct gaacggcgcg ggcaatacct
     5881 tcggcagggg cgaccggttc gtcaatgctg ctggtgatgt cgatggtgcc gcctttgcgc
     5941 gcgaactcca gcgcctgctc aaacaacgga acgttgcggt taacgtgggt cggcagcagc
     6001 ttgctgatcg gcacatcgca gttttccagc aggtcataga caggctgtaa cgcctttttg
     6061 ctgtcaccca tgtggaacac ggtgacgcca ggtttaccgc cgagcaaacc gccaacgcgg
     6121 gattccgccg ccatattagc caggtgataa acgtccggtg cggcagaacg gtgatcagag
     6181 atggcgcatt tcacaccaat cacgcgatcg ataatcgcca cgtctttttc cacggaaccc
     6241 gtaatagtgc gggaagggac atgataagcg ccggtcagca tccaggcgct gatgccttct
     6301 tcattgagcg cacgggtctt ggcgagcagg gattccgggt ggcgagagat agagtcggtg
     6361 cccagcaggc caaccactga cgtgacgccc gcttccgtca gacgacttaa cgccacttct
     6421 ggcgtgcgtg tcgtgggacc tgcttcgccg ccaccaccaa tcaaatggac gtgttgatca
     6481 ataaaacctg ggcagaggat ctgcccgctg agatcgacaa ccgtgcagtc cggtacaatg
     6541 tcagaaggga tgttgctggc aacggcaata attttgccgt tagcgatgag gacatcgcaa
     6601 attccccgat cttccggcgc atacaaatgc gctccctgca gcagggtaaa accggctgcg
     6661 gaataatcaa tcatggtaac tccttgttaa acaataagct gcataaccca gatcgacagc
     6721 aatgcgttaa tcacgcagac cgtaatgata tgcggataat atttggcatt cacttcggct
     6781 gtccccaggc agcgaccgac attttgcact gggttgccca ttaaatacat cgcgggcagt
     6841 aaaacggtga catcgtggcc ggttaatgcg ccagcggttg ccagactggc ggcaacaccg
     6901 actgcgccgc ccatactcat taatgcggct aaaaggacgg ttgccgcttc accgggtaat
     6961 ccccacagcg ccattacggg ttcacagata tgtcccaccc agtcgagcag gccagtaatt
     7021 ttcagcgcct ggatgatgac aaaggccatc accacgttcg gcagcaggtt ggtggtggcg
     7081 atagtaaagc cacggcgtgc gccatcgata aacatatcca tgacattttt gcggacctga
     7141 gttgtcatgc ttgcgctcct tgcgttgggt tgcgacgttc ttcgaagtta agccagacgc
     7201 gcaaaatgtt ggctccgaca aatttaaaca ccaggatgac ggctaaaggt acgattacgg
     7261 acgtgcccag aaaggcaaac accgcgacgc cggaagagaa gtagttggtg atgatggcgc
     7321 taccgctggt ttgataggcg gcgaatatca ctttatcacg ttcggtaatt tccccttcct
     7381 gcgccagttc tttggtcata cctgcggcgg catcggtgtt ttgcaggtta gcaatcagcg
     7441 ccagtgaaca gatgcctgga atgcccaaca acggttttag cacgggcgtc attaactgct
     7501 gcgcggcgcg caatccgcca agaccatcgg taatcgagat aattcccaga gagagaatca
     7561 ctgacggagc cagttccagc gcgaagagaa agccatcttt agctccagtg ccacctgcgc
     7621 cgcgaaacga ggtggtagcg ccattcgcac caggcagttg cccaaacgat ccgttcagaa
     7681 cggaaaagtc aaaaacgcgc caccaactgt ctgtcccgga aaaaactccg gaaaaaaaga
     7741 taatcgtgag aaaaaaggcc agataccctt taacacccac tttttctgtc gccagctccc
     7801 cagctacagc atccccttgt tgtgtcataa caatccccat tgtttttttg tgtatatgtg
     7861 ttgcttgagc caggaagatt cctggagctg aaaaacctta tcagtcatgt gggtatgggg
     7921 aaaatgcaag tatgtttctt gctatgcgtt ttttgcatag cggaaaaatg acgctgtgtg
     7981 gcaaaggttc tgcgcaggat tgagttgcag aaaattcata aacccagtga caatgacatc
     8041 agtaaataaa aacagaaaat tcagggacga aaacgcgatg gcaaaataca acgacaaaga
     8101 gctggcagag acgagcaagt ttttaagttt tgtcctccga cataaaccag aggcgattgg
     8161 catcgtgctg gaccgtgaag gatgggcgga tatcgataag ctcatacttt gtgcccaaaa
     8221 agcgggcaag cggttaaccc gcgcattact ggatactgtg gtcgccacca gcgataaaaa
     8281 gcgctttagt tattccagtg atggtacgtg tattcgcgcc gttcaggggc attcgacttc
     8341 gcaggtcgtc attgccttca tcgaaaaaac accgccacaa tttctctacc acggtacggc
     8401 aagccgtttc cttgaggcga taaaaaaaca ggggctgatt gcaggggagc gccattacgt
     8461 gcatctctct gctgatgaag caacggcccg caaagtcggg gcaagacacg gttcgccggt
     8521 tatcttaacc gtcaaagcac aggaaatggc gaaacgaggg ctccctttct ggcaagcgga
     8581 aaacggggtc tggctgacat caacggttgc ggttgagttt cttgagttgt aagggaaact
     8641 tccctcatga agtgaccact tgtagcttaa ataattgcac tgtgctgata agcgcagcaa
     8701 taaacaacgc cgccgcgccg cagagagtgg cgccggcaag cgtcgatgtt ccattgccaa
     8761 gcatactggt cagcgccggg ccaagaattt gcccaatgcc gtaaatgagc gtcacaaagc
     8821 ctaaaagatt gagatttccc ggtacgctca gttggcgggc gatagtcatc accagagatg
     8881 tcgtgcccat aaaggtactg ccaaaaccaa tactgctgat gataagcaag agaggcgagt
     8941 cgctggcgag agtaagcaac acactaatag cctgaaccag caaattcgcc gtcaggcagg
     9001 gcaaagcccc ccaacgtttt gctgcccata gccagccaaa gcaaccaggc acaattgata
     9061 agccgactaa cgtccagaga tgggcggtta acaatggtga gcctgcatct ttcgccatga
     9121 gcggcaggta agtcgcgacg atgatataac caaatcccgc caggccgtac agaatagcca
     9181 gtaaccacca gctcattatc tgttgctccg tttttgccaa tggcattggt gtgatggcgt
     9241 gttttttcga gggcattaaa agcgtaagtg caatcagcat catgccagaa agcgccccgg
     9301 cgccttgcca taacgtttgc gaggagaggt caaaatgcag gcctgccaga acatattcat
     9361 tgcccagtgc gatgccaatg ccaacgccag aaaacaaagc tgccagcaca aaaggatggc
     9421 gcgtgtgctg cataatcagc gtcgaaccaa aaatcagcat tccggcgctg gcgacacccg
     9481 ccaggacgcg aatcaataac accagaataa atggcagcaa ccatgccatt gcgaggatca
     9541 acaacccgct cgccagggca gaagccaaca gaaatgggcg caggcgcgat ggctggtgaa
     9601 atgcgccaaa cgaaaatagc agactgccag ccagataccc cgcatagttg ccgctggcaa
     9661 tccacgagag ctggctaaat gagaacgatc cttccgccat catgacgggc aacataggcg
     9721 tataaaggaa gcggcctaaa cccataccta aggttagaac cagcatcccg aaaagcgcgg
     9781 tgctgaaaga aaagcgttct acgggatgcg tggatgaagg catgtttaac cttgtttgtc
     9841 atttttgttg ttaacaagtt aataacaaaa aggtaaaaca tgccagcctg ttaatgcgat
     9901 gattgagggg taaagcgata aaaacgattt ggttcgctaa cgatataaag attaccagag
     9961 gcatccatcg caaccccttc ggcctgtttg atgttatgcg acaaacctcg actgcctttg
    10021 gttagtgaca tttcaccgat aacctcgcca accagcgtta cttcctggag tgcgcgagat
    10081 tcatgagaca gcaccagaag cgtatttttt tgttggttaa attctgcgcc ggacacatca
    10141 tctaaagtaa attgccgttg tagtgccttg tctttgctga tgtgcaattc attgctactt
    10201 aatagcccgt taactttgta gacctcaatc gggtttttct ctttaaaaaa ccagaaagtg
    10261 tgatcctggc gagaataggc caatccttca aaaccgcaat tggttgggga ttcctgcagc
    10321 gggattttta tttttttgag gatttttact tccgagtttg gggtcagtga aatcacataa
    10381 atggcgtaat cgcgctcatc gctaatgaca aactgattat cgccaatgta ttcgatcgtc
    10441 tcgagatcct taacaaaatc cagaggaatt gtgcggatca aatcaccatt ggtggtcatt
    10501 tcaacaatgg cggcaggttt gttgatagtg ctaaaaagag tattgctttg tgccgaccag
    10561 gtcagcgaag agatattatt cgttatgcct gcaatttctt tgccgtcaat ggtggcgtgg
    10621 taattctgaa atgatgctgc atgattgctt ttacgggcgc aagattgcac gaagaaagta
    10681 cacactgcca caatgacaaa aagaatcaca ataacgaaga tgcgtttact taacgaaata
    10741 ctttttgtca cggtaatccc tttaaggcaa atcaataaga tatcgagaag tatacgtagc
    10801 gatcttaatg aatccttaac gcggagaaat cccgtaatgt gcaatgcagc gccagccacc
    10861 atcctgctga caataaactg attccgtatg cccagggatc acccattgaa tttcctcgcc
    10921 aggcggacag gtcagccaaa tgcataaccc gcagccgccg cgtaaatcac ggggaatatc
    10981 gctgacgcga aaagtcatgc ccgctgcctg caacgccttg cgggtttgta tgacgccgac
    11041 cgtggagtga aataaaaaca gatactcttt catgcgcgtt ttctctgccg ctgaccgatc
    11101 accgccgcgc caattgcacc agcgaattgc gcatcagggt gagtttgtac cggcattccc
    11161 agatggcttt ccagcatgtg ggtaaacctt tgacagtggc taacgccacc agtaaacaga
    11221 atcggcgctt cacaggagag acgagcaatg aaattggcgc tccgccgcgc catcgcgtta
    11281 atcactccgg cgagaatcgc ttctggcgct acgcccgcag agcgcaggct gatcacttct
    11341 gattcagcaa acactgtgca catactcgtg atggcgtgcg gtatgacatt ttcggtaatg
    11401 ctgtcgagtt gctcgacgct ggtgccaagc gtgcgcgaga tcacctccag gaaacgcccg
    11461 gtgcccgccg cgcatttgtc attcatcagg aaatcgcaca ggttaccgtc atcatcaagc
    11521 tgaatcactt tgctgtcctg accaccgatg tcgattaccg cgcgcgttgc tggctcaaga
    11581 aaccgtgcgc ccagcccgtg acaggagatt tccgtcactt gtttatcggc aaaatccacc
    11641 agttgccgtc cgtagccggt gagcgtcaga aacggcgttg tctctaaccc ttcgcgcaga
    11701 gtttcccagg cttcagtaat tgctgttgcc gggcgaaagg gtgttggaac gaggaaacgg
    11761 cgcgtaatca cgccgtcagc cagtaagatc cctttggtgg cggttgagcc ggaatcaatg
    11821 ccaatcgaat atgtcactgc cactccttac agcatctcaa taaaggccgc gacacgggtg
    11881 ctgagctgcc cgacatccga ggtggagtag tctgtttcaa tagcgatata aggaatgttg
    11941 tgctgctggc gcacatgacg tttaatcgcc agcgattcca ccgcgtaggt atggcacgcc
    12001 tgcaaaatca catcaactac gccatcgacc tgatattctt caaccatctg gctgagcatt
    12061 tgcaggcgct gatcgttcgg cgaaacacag gagcagccaa tcgccagata tttatccgcc
    12121 agcgcgtcgt acacatcacc cgtttccgcc acgcattgct cggtggcttt cgccccggtg
    12181 cagttttcat aaccgacaac ccagccgcca ttctcttcaa tcgcgcgcac cactttctct
    12241 gctgcgccgc caattgggca gccggtgatt aaaatacgtg ggcgcgggcc cagtcgctgg
    12301 ccttcttccc actgctgacg aacgcgggcg gtcattgcat ccagttcatt gatcaacgcc
    12361 tctttatcga accggaaggt tgcgccgtag actactttca gaatgtcgct gccgctaagc
    12421 gccggaggat ttaactgccc caaatgataa aagttggcca gtgcgcgacg ttcgcggttt
    12481 ttcagcgcaa tggcatcgcg cagagcatct tcgctaatct cgtgcccaaa acgttcttct
    12541 accgtttttt gcaagcgcag catctcggct ttccataatg cacgcgaggc atcgtcctta
    12601 acgctgtttg gcagttgcat cacatgaacg ggcttaaact ccgccatgta ttcatacatt
    12661 ttctttttgc cgtcgcaggt ggtttcaccg accaccagat cagaaaagta gaagtagggg
    12721 catttatcgg ttttgccgaa gccgtagctg cttttaatca gcgggcagag gttgcgcggc
    12781 agatctttct cggcttcttc aatggtttca tcagaggtgg aacatagcga aaccacaacc
    12841 gcaccggctg ccatcgggat ctcttgcggc ataaaggtgc agtaggtgcc aaccagcgga
    12901 atgccgcgct ccttgagatc catgacggtg agaaagcctt tctggcgagc ttcagagaac
    12961 tgatcgaaaa tggcgggtag atcggtaaca agtgacatga ttttccttcc ccgtaccacg
    13021 ggagataatg aaaagaggcc acattattac cattctttta gtgtggttta tttgatctct
    13081 ctcgcggtta gccacttagt ttttcatgga tttctataaa ttgccgaggg ggaacaaggc
    13141 cgggagctgc gacagcaaat acaaaataag tccaatacaa ccgccaacca gcgtaccgtt
    13201 gacacggata aactggagat ctttgccgat atttaactcg atttgccgcg acatatcccg
    13261 tgcgtcccag ctttttaccg tatcgctaat gtggcgagtc aggaatgcgg aaaactcagg
    13321 cgcaacgcgg tgtgcggctt gttccagatg accatttaac gacgcccgca aggcatcatc
    13381 ggcaattaac gtttcgccaa accattgacc cgcccgcgcg atacgttctt tcacacgaga
    13441 atcttcactg ttgatatccg ctttcagcca ttcccgtaaa tccccccaca attcactgag
    13501 atagcggtta aaagcttcat cttctttcag atagcttttt acggcatcgg ctcgcgctgc
    13561 catttccgga tcgtttttca gcttgtcgat cagggcaaaa gtggcgcgat caaacgcatg
    13621 gcggatctga tgcgcacgat cgcggctgat atcatcaagc aaagaattca ccgcgtcaga
    13681 aaccaactcc gcgctatgtt cgcccagcca ttcagtgggc aaaattttgg ctttcagtgg
    13741 atgctcgctc tccagccagc gaacaatttg ctgggcgata aacttgcgcg atttatcgcg
    13801 ctggagaagg gcgatcaact gtgcgatcag cgtatcgagc agcacctgat gacgatcgtt
    13861 tctggtcatg ctctccagaa tcagtgcgct ggtgccggaa agatcgacct tatcgatcgc
    13921 ccggtggacc gcgcgcttaa gcaggcgctg aatacgcgca tcatcggtca gttcgagaaa
    13981 accgctcatg atctgtaaca gatgctgacc aacgcggcgg gcgttctccg gctgactaaa
    14041 ccagttgcca atcaacaacg ccggttcgtg acgtcgaatc aatgccacca gcgattgggt
    14101 atcgagaaat ttttcctgca cgaactggcc gagattttcg ccaatccggt ctttattacg
    14161 cgggataatc gccgtatgac gagaaatgat cggaatcggc acgcggcgaa acagcgccac
    14221 caccgcaaac cagtccgcca gcgcgccgac catcgccgct tcagcaatcg ccttcacacc
    14281 gctcacccaa aaattgggcg gcaaaaacag cgtaacgaca aaggtagcag cggcgataag
    14341 cagtaaagag agcgccaaca ttttggcgcg tctgagttca atgagtttat tcatatcgta
    14401 agaatagagc ctgacggtaa gaaaacgcaa aaagcgagat atcttccata aaaatgtatc
    14461 gtttgcagaa gaataaaaat agcctggaaa ccgcctcggg gaataggaaa tagccaggtg
    14521 tgaatggctt tgttagtcgc taaagttggg ccatcttatc tacaggtgac ggatcgccat
    14581 gacaaacttt acgaccagca cgccgcatga cgcattattt aaatcctttc tcacacactc
    14641 tgacaccgcg cgggatttca tggagatcca cttacccaaa gatttacgtg aactgtgcga
    14701 tctcgatagc ttaaaactgg aatccgccag ctttgtcgat gaaaaattgc gggcgctaca
    14761 ttccgatatt ctgtggtcgg taaagacccg tgaaggcgat ggctatattt atgtagtgat
    14821 tgaacatcag agccgtgagg atattcatat ggcctttcgc ctaatgcgat attccatggc
    14881 ggtgatgcag cgccatatcg aacatgataa acgccggcag ctaccgctgg tcatcccgat
    14941 gctgttttat cacggtagcc gtagtcctta tccctggtcc ctgtgctggc tggacgaatt
    15001 tgccgacccg actaccgcac ggaagcttta taccgccgcg ttcccgctgg tggatatcac
    15061 tgtcgtgcca gacgacgaga ttgtgcagca tcgcagagtc gccctgctgg agttgatcca
    15121 aaagcatatt cgccagcgcg atctaatggg gcttattgat caactggcag tattactggt
    15181 tacagggtgc gctaatgaca gccagataac cgcgctgtta aattacattt tactggctgg
    15241 cgatgaagcg cgttttaaag agtttatcag tgaacttacc cgtcgaatgc cacaacacag
    15301 ggagcgaata atgacgattg cagagcgaat tcataatgat ggatatataa aaggggaaca
    15361 gcgcattctt cgattgtttt tgcagaatgg ggtagatcct gaatggatac aaaagattac
    15421 cggactttcg gcagagcaaa tacaggcatt agagcagccc ttgcctgacc gtgagcacga
    15481 ttcatggctc gagagctaat atggaactat cgctatgtgc ctccagcata tccagcacct
    15541 gatcccactg ggtaatttcc accggacgcg tcatcgacgg tactggcggc tgggctttac
    15601 gttgcgcgac aaaataaccc gaacgcggct acggagtgat gaggttcatc gtctccagcg
    15661 tctgatacgc ctgctgcacg gtgctgacgc cgtgctcctg actcaagcta cgcaccgacg
    15721 gcaatttctc cccgtgacga tacagccctt gctcaatccg ctcggcaagc agagttgcca
    15781 gatgttgata acgcgtcatg ctgtattcct tatttggacc atacagagag aaaaaccagt
    15841 acagatgagg ctaaaaaacc ggatgcagat gctgttttaa cctgtctgta tgttaaataa
    15901 aactaatatt tgcatctgta ttgtgaactg ccagaagacg gatgatgaag cctctgaaag
    15961 atgaggaagg cagcagatgg aatttcacta aaacaaagcc aaagcgccgt ttatcggttt
    16021 cgtacaactc tggcaagcgg tgaggagctg gtggcggcaa atgcaggccc gacgggtgtt
    16081 acagcagatg agtgatgagc ggttgaggga tattgggtta cgcagggagg atgtggagtg
    16141 atagaggatg ctgagaataa atcaggatat taacttttta ta
//
LOCUS       DADSYM010000112        15912 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2415
            SAMN08039579-rid11723173.denovo.112, whole genome shotgun sequence.
ACCESSION   DADSYM010000112 DADSYM010000000
VERSION     DADSYM010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039579
            Sequence Read Archive: SRR6362533
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15912)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15912)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980162.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 03:39:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,484
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 4,347
            CDSs (with protein)               :: 4,347
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 43 of 85
            Pseudo Genes (incomplete)         :: 37 of 85
            Pseudo Genes (internal stop)      :: 19 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15912
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039579-rid11723173.denovo.112"
                     /strain="LGE2415"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8302 N 95.9628 W"
                     /collection_date="2015-08-24"
     gene            complement(<1..1328)
                     /gene="uidB"
                     /locus_tag="J1Y83_003157"
     CDS             complement(<1..1328)
                     /gene="uidB"
                     /locus_tag="J1Y83_003157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronide transporter"
                     /protein_id="HBA7631282.1"
                     /translation="MNQQLSWRTIVGYSLGDVANNFAFAMGALFLLSYYTDVAGVGAA
                     AAGTMLLLVRVFDAFADVFAGRVVDSVNTRWGKFRPFLLFGTAPLMIFSVLVFWVPTD
                     WSHSSKVVYAYLTYMGLGLCYSLVNIPYGSLATAMTQQPQSRARLGAARGIAASLTFV
                     CLAFLIGPSIKNSSPEEMVSVYHFWTIVLAIAGMVLYFICFKSTRENVVRIVAQPSLK
                     ISLQTLKRNRPLFMLCIGALCVLISTFAVSASSLFYVRYVLNDTGLFTVLVLVQNLVG
                     TVASAPLVPGMVARIGKKNTFLIGALLGTCGYLLFFWVSVWSLPVALVALAIASIGQG
                     VTMTVMWALEADTVEYGEYLTGVRIEGLTYSLFSFTRKCGQAIGGSIPAFILGLSGYI
                     ANQAQTPEVIMGIRTSIALVPCGFMLLAFVIIWFYPLTDKKFKEIVVEID"
     gene            complement(1325..3136)
                     /gene="uidA"
                     /locus_tag="J1Y83_003158"
     CDS             complement(1325..3136)
                     /gene="uidA"
                     /locus_tag="J1Y83_003158"
                     /EC_number="3.2.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucuronidase"
                     /protein_id="HBA7631283.1"
                     /translation="MLRPVETPTREIKKLDGLWAFSLDRENCGIDQRWWESALQESRA
                     IAVPGSFNDQFADADIRNYVGNVWYQREVFIPKGWAGQRIVLRFDAVTHYGKVWVNNQ
                     EVMEHQGGYTPFEADVTPYVIAGKSVRITVCVNNELNWQTIPPGMVITDENGKKKQSY
                     FHDFFNYAGIHRSVMLYTTPNTWVDDITVVTHVAQDCNHASVDWQVRANGDVSVELRD
                     ADQQVVATGQGTSGTLQVVNPHLWQPGEGYLYELCVTAKSQTECDIYPLRVGIRSVAV
                     KGEQFLINHKPFYFTGFGRHEDADLRGKGFDNVLMVHDHALMDWIGANSYRTSHYPYA
                     EEMLDWADEHGIVVIDETAAVGFNLSLGIGFEAGNKPKELYSEEAVNGETQQAHLQAI
                     KELIARDKNHPSVVMWSIANEPDTRPQGAREYFAPLAEATRKLDPTRPITCVNVMFCD
                     AHTDTISDLFDVLCLNRYYGWYVQSGDLETAEKVLEKELLAWQEKLHQPIIITEYGVD
                     TLAGLHSMYTDMWSEEYQCAWLDMYHRVFDRVSAVVGEQVWNFADFATSQGILRVGGN
                     KKGIFTRDRKPKSAAFLLQKRWTGMNFGEKPQQGGKQ"
     gene            complement(3526..4116)
                     /locus_tag="J1Y83_003159"
     CDS             complement(3526..4116)
                     /locus_tag="J1Y83_003159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBA7631284.1"
                     /translation="MMDNMQTEAQPTRTRILNAAREIFSENGFHSASMKAICKSCAIS
                     PGTLYHHFISKEALIQAIILQDQERALARFREPIEGIHFVDYMVESIVSLTHEAFGQR
                     ALVVEIMAEGMRNPQVAAMLKNKHMTITEFVAQRMRDAQQKGEISPDINTAMTSRLLL
                     DLTYGVLADIEAEDLAREASFAQGLRAMIGGILTAS"
     gene            complement(4345..5112)
                     /gene="hdhA"
                     /locus_tag="J1Y83_003160"
     CDS             complement(4345..5112)
                     /gene="hdhA"
                     /locus_tag="J1Y83_003160"
                     /EC_number="1.1.1.159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-alpha-hydroxysteroid dehydrogenase"
                     /protein_id="HBA7631285.1"
                     /translation="MFNSDNLRLDGKCAIITGAGAGIGKEIAITFATAGASVVVSDIN
                     ADAANHVVDEIQQLGGQAFACRCDITSEQELSALADFAVSKLGKVDILVNNAGGGGPK
                     PFDMPMADFRRAYELNVFSFFHLSQLVAPEMEKNGGGVILTITSMAAENKNINMTSYA
                     SSKAAASHLVRNMAFDLGEKNIRVNGIAPGAILTDALKSVITPEIEQKMLQHTPIRRL
                     GQPQDIANAALFLCSPAASWVSGQILTVSGGGVQELN"
     gene            complement(5224..6252)
                     /locus_tag="J1Y83_003161"
     CDS             complement(5224..6252)
                     /locus_tag="J1Y83_003161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mal regulon transcriptional regulator MalI"
                     /protein_id="HBA7631286.1"
                     /translation="MATAKKITIHDVALAAGVSVSTVSLVLSGKGRISTATGERVNAA
                     IEELGFVRNRQASALRGGQSGVIGLIVRDLSAPFYAELTAGLTEALEAQGRMVFLLHG
                     GKDGEQLAQRFSLLLNQGVDGVVIAGAAGSSDDLRRIAEEKAIPVIFASRASYLDDVD
                     TVRPDNMQAAQLLTEHLIRNGHQRIAWLGGQSSSLTRAERVGGYCATLLKFGLPFHSD
                     WVLECTSSQKQAAEAITALLRHNPTISAVVCYNETIAMGAWFGLLKAGRQSGESGVDR
                     YFEQQVSLAAFTDATPTTLDDIPVTWASTPARELGTTLADRMMQKITHEETHSRNLII
                     PARLIAAK"
     gene            6427..8019
                     /locus_tag="J1Y83_003162"
     CDS             6427..8019
                     /locus_tag="J1Y83_003162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS maltose transporter subunit IICB"
                     /protein_id="HBA7631287.1"
                     /translation="MTAKTAPRVTLWEFFQQLGKTFMLPVALLSFCGIMLGIGSSLSS
                     HDVITLIPVLGNPVLQAIFTWMSKIGSFAFSFLPVMFCIAIPLGLARENKGVAAFAGF
                     VGYAVMNLAVNFWLTNKGILPTTDAAVLKANNIQSILGIQSIDTGILGAVIAGIIVWM
                     LHERFHNIRLPDALAFFGGTRFVPIISSLVMGLVGLVIPLVWPIFAMGISGLGHMINS
                     AGDFGPMLFGTGERLLLPFGLHHILVALIRFTDAGGTQEVCGQTVSGALTIFQAQLSC
                     PTTHGFSESATRFLSQGKMPAFLGGLPGAALAMYHCARPENRHKIKGLLISGLIACVV
                     GGTTEPLEFLFLFVAPVLYVIHALLTGLGFTVMSVLGVTIGNTDGNIIDFVVFGILHG
                     LSTKWYMVPVVAAIWFVVYYIIFRFAITRFNLKTPGRDSEVASSIEKAVAGAPGKSGY
                     NVPAILEALGGADNIVSLDNCITRLRLSVKDMSLVNVQALKDNRAIGVVQLNQHNLQV
                     VIGPQVQSVKDEMAGLMHTVQA"
     gene            8029..9201
                     /gene="malY"
                     /locus_tag="J1Y83_003163"
     CDS             8029..9201
                     /gene="malY"
                     /locus_tag="J1Y83_003163"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional maltose regulon transcriptional
                     repressor/cystathionine beta-lyase MalY"
                     /protein_id="HBA7631288.1"
                     /translation="MFDFSKVVDRHGTWCTQWDYVADRFGTADLLPFTISDMDFATAP
                     CIIEALNQRLIHGVFGYSRWKNDEFLAAIAHWFSTQHYTAIDPQSVVYGPSVIYMVSE
                     LIRQWSETGEGVVIHTPAYDAFYKAIEGNQRTVMPVALEKQPDGWFCDMGKLEAVLAK
                     PECKILLLCSPQNPTGKVWTCDELEIMADLCERHGVRVISDEIHMDMVWGEQPHIPWS
                     NVARGDWALLTSGSKSFNIPALTGAYGIIENSSSRDAYVSALKGRDGLSSPSVLALTA
                     HIAAYQQGAPWLDALRVYLKDNLTYIAVKMNAAFPELNWQIPQSTYLAWLDLRPLNID
                     DNALQKALIEQEKVAIMPGYTYGEEGRGFVRLNAGCPRSKLEKGVAGLINAIRAVR"
     gene            9305..10306
                     /gene="add"
                     /locus_tag="J1Y83_003164"
     CDS             9305..10306
                     /gene="add"
                     /locus_tag="J1Y83_003164"
                     /EC_number="3.5.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HBA7631289.1"
                     /translation="MIDTTLPLTDIHRHLDGNIRPQTILELGRQYNISLPAQSLETLI
                     PHVQVIANEPDLVSFLTKLDWGVKVLASLDACRRVAFENIEDAARNGLHYVELRFSPG
                     YMAMAHQLPVSGVVEAVIDGVREGCRTFGVQAKLIGIMSRTFGEAACQQELEAFLAHR
                     DQITALDLAGDELGFPGSLFLSHFNRARDSGWHITVHAGEAAGPESIWQAIRELGAER
                     IGHGVKAIEDRALMDFLAEQQIGIESCLTSNIQTSTVADLAAHPLKTFLEHGIRASIN
                     TDDPGVQGVDIIHEYTVAAPAAGLSREQIRQAQINGLEMAFLSAEEKRALREKVAAK"
     gene            complement(10342..11382)
                     /locus_tag="J1Y83_003165"
     CDS             complement(10342..11382)
                     /locus_tag="J1Y83_003165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310359.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HBA7631290.1"
                     /translation="MSDNIRVGLIGYGYASKTFHAPLIAGTPGLELAVISSSDETKVK
                     ADWPTVTVVSEPKHLFNDPNIDLIVIPTPNDTHFPLAKAALEAGKHVVVDKPFTVTLS
                     QARELEALAKSLGRVLSVFHNRRWDSDFLTLKGLLAEGVLGEIAYFESHFDRFRPQVR
                     DRWREQGGPGSGIWYDLAPHLLDQAITLFGLPVSMTVDLAQLRPGAQSTDYFHAILSY
                     PQRRVILHGTMLAAAESARYIVHGSRGSYVKYGLDPQEERLKNGERLPQEDWGYDMRD
                     GVLTRVEGEERVEETLLTVPGNYPAYYAAIRDALNGDGENPVPASQAIQVMELIELGI
                     ESAKHRATLCLA"
     gene            11625..11750
                     /gene="blr"
                     /locus_tag="J1Y83_003166"
     CDS             11625..11750
                     /gene="blr"
                     /locus_tag="J1Y83_003166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division septum protein Blr"
                     /protein_id="HBA7631291.1"
                     /translation="MNRLIELTGWIVLVVSVILLGVASHIDNYQPPEQSTTVQHK"
     gene            12023..12238
                     /gene="ydgT"
                     /locus_tag="J1Y83_003167"
     CDS             12023..12238
                     /gene="ydgT"
                     /locus_tag="J1Y83_003167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription modulator YdgT"
                     /protein_id="HBA7631292.1"
                     /translation="MTVQDYLLKFRKISSLESLEKLYDHLNYTLTDDQELINMYRAAD
                     HRRAELVSGGRLFDLGQVPKSVWHYVQ"
     gene            12324..12764
                     /locus_tag="J1Y83_003168"
     CDS             12324..12764
                     /locus_tag="J1Y83_003168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707526.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2569 domain-containing protein"
                     /protein_id="HBA7631293.1"
                     /translation="MTTTTPQRIGGWLLGPLAWLLVALLSTTLALLLYTAALSSPQTF
                     QTLGGQALTTQILWGVSFITAIAMWYYTLWLTIAFFKRRRCVPKHYIIWLLISVLLAV
                     KAFAFSPVEDGIAVRQLLFTLLATALIVPYFKRSSRVKATFVNP"
     gene            12841..13422
                     /gene="rsxA"
                     /locus_tag="J1Y83_003169"
     CDS             12841..13422
                     /gene="rsxA"
                     /locus_tag="J1Y83_003169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017456621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxA"
                     /protein_id="HBA7631294.1"
                     /translation="MTDYLLLFVGTVLVNNFVLVKFLGLCPFMGVSKKLETAMGMGLA
                     TTFVMTLASICAWLIDTWILIPLNLIYLRTLAFILVIAVVVQFTEMVVRKTSPVLYRL
                     LGIFLPLITTNCAVLGVALLNINLGHNFLQSALYGFSAAVGFSLVMVLFAAIRERLAV
                     ADVPAPFRGNAIALITAGLMSLAFMGFSGLVKL"
     gene            13422..14000
                     /gene="rsxB"
                     /locus_tag="J1Y83_003170"
     CDS             13422..14000
                     /gene="rsxB"
                     /locus_tag="J1Y83_003170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxB"
                     /protein_id="HBA7631295.1"
                     /translation="MNAIWIAVAAVSLLGLAFGAILGYASRRFAVEDDPVVEKIDEIL
                     PQSQCGQCGYPGCRPYAEAISCNGEKINRCAPGGEAVMLKIAELLNVEPQPLDGEAPE
                     LTPARMVAVIDENNCIGCTKCIQACPVDAIVGATRAMHTVMSDLCTGCNLCVDPCPTH
                     CISLQPVAETPDSWKWDLNTIPVRIIPVEHHA"
     gene            13993..>15912
                     /gene="rsxC"
                     /locus_tag="J1Y83_003171"
     CDS             13993..>15912
                     /gene="rsxC"
                     /locus_tag="J1Y83_003171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxC"
                     /protein_id="HBA7631296.1"
                     /translation="MLKLFSAFRKNKIWDFNGGIHPPEMKTQSNGTPLRQVPLAQRFV
                     IPLKQHIGAEGELCVSVGDKVLRGQPLTRGRGKMLPVHAPTSGTVTAIAPHSTAHPSA
                     LAELSVIIDADGEDCWIPRDGWADYRSRSREELIERIHQFGVAGLGGAGFPTGVKLQG
                     GGDKIETLIINAAECEPYITADDRLMQDCAAQVVEGIRILAHILQPREILIGIEDNKP
                     QAISMLRAVLADSHDISLRVIPTKYPSGGAKQLTYILTGKQVPHGGRSSDIGVLMQNV
                     GTAYAVKRAVIDGEPITERVVTLTGEAIARPGNVWARLGTPVRHLLNDAGFCPSADQM
                     VIMGGPLMGFTLPWLDVPVVKITNCLLAPSANELGEPQEEQSCIRCSACADACPADLL
                     PQQLYWFSKGQQHDKATTHNIADCIECGACAWVCPSNIPLVQYFRQEKAEIAAIRQEE
                     KRAAEAKARFEARQARLEREKAARLERHKSAAVQPAAKDKDAIAAALARVKEKQAQAT
                     QPIVIKAGERPDNSAIIAAREARKAQARAKQAELQQTNDAATVADPRKTAVEAAIARA
                     KARKLEQQQANAEPEQQVDPRKAAVEAAIARAKARKLEQQQANAEPEEQIDPRKAAVE
                     AAIARAKARKLEQQQA"
BASE COUNT         3791 a         4170 c         3956 g         3995 t
ORIGIN      
        1 ttatcaattt caacaacgat ttctttgaat tttttatccg tgagcggata aaaccagata
       61 atgacgaatg ccagtagcat aaatccgcaa ggtactaagg caattgatgt gcggatgccc
      121 ataataactt ccggcgtttg cgcctgattg gcgatatatc cgcttaatcc caaaataaag
      181 gcaggaattg aacctccgat tgcctgaccg catttacggg taaatgagaa tagtgaatag
      241 gtgagccctt caattcgcac gccggtcagg tattcaccgt attctacggt atcagcttcc
      301 agtgcccaca tcacggtcat ggtaacgccc tggccaattg atgcgatggc caacgcaacc
      361 aacgccaccg gcagtgacca gacggaaacc cagaagaaca gcagataacc gcaggttccc
      421 agcaaagcgc caatcaggaa ggtattcttt ttaccgatcc tcgcgaccat ccccggcacc
      481 agcggtgccg atgccacagt accgaccagg ttttgcacca gtaccagcac agtgaacagc
      541 ccggtatcat ttaacacata gcgcacgtag aacaacgacg aggcgctgac cgcgaaggtc
      601 gaaatcagca cacacagcgc accgatgcac aacataaaca gcgggcgatt ccgtttcagg
      661 gtttgcagac tgatcttcaa tgacggctgc gccacgatac gtaccacatt ctcacgcgtc
      721 gatttgaagc agatgaagta aagcaccatt ccggcaatcg ccagcacaat tgtccagaaa
      781 tgatataccg acaccatctc ttccgggctg gagttcttaa tgctcggtcc tatcagaaat
      841 gccaggcaga caaaagtcaa tgaagcggca atcccgcgag ccgcgcccag acgggcgcgg
      901 gattgtggtt gttgggtcat cgcggtagca agtgaaccat aaggaatatt caccaggctg
      961 tagcaaagcc cgagtcccat gtaggtcaaa tatgcataca caactttgct gctatggctc
     1021 cagtcggtcg gcacccagaa taccagcacg ctgaagatca ttaacggcgc agtaccgaag
     1081 agtaaaaacg ggcggaattt tccccatcgg gtattcacac tgtccaccac tcgtccggca
     1141 aagacatcgg cgaaggcatc gaatacccgc accagtaaca gcatggtgcc cgccgcagcg
     1201 gcaccgacgc cagcgacgtc ggtgtagtaa ctcaacagga agagcgcccc cattgcgaag
     1261 gcgaagttat tggcgacgtc accgaggctg tagccgacga tggtgcgcca ggagagttgt
     1321 tgattcattg tttgcctccc tgctgcggtt tttcaccgaa gttcatgccg gtccagcgtt
     1381 tttgcagcag aaaagccgcc gacttcggtt tgcggtcgcg ggtgaagatc cctttcttgt
     1441 taccgccaac gcgcaatatg ccttgcgagg tcgcaaaatc ggcgaaattc catacctgct
     1501 caccgacgac agcgctgacg cgatcaaaga cgcggtgata catatccagc catgcacact
     1561 gatactcttc actccacatg tcggtgtaca ttgagtgcag gccggctaac gtatccacac
     1621 cgtattcggt gatgataatc ggctgatgca gtttctcctg ccaggccaga agttcttttt
     1681 ccagaacctt ctctgccgtt tccaaatcgc cgctttggac ataccaaccg taataacggt
     1741 tcaggcacag cacatcaaag agatcgctga tggtatcggt gtgagcgtcg cagaacatta
     1801 cattgacaca ggtgatcgga cgcgtcgggt cgagtttacg cgttgcttcc gccagtggcg
     1861 cgaaatattc ccgtgcacct tgcggacggg tatccggttc gttggcaata ctccacatca
     1921 ccacgcttgg gtggtttttg tcacgcgcaa tcagctcttt aatcgcctgt aagtgcgcct
     1981 gctgggtttc cccgttgact gcctcttcgc tgtacagttc tttcggcttg ttgcccgctt
     2041 cgaaaccaat gcctaaagag aggttaaagc cgacagctgc agtttcatca atcaccacga
     2101 tgccatgctc atctgcccag tcgagcatct cttcagcgta agggtaatgc gaggtacggt
     2161 aggagttggc cccaatccag tccattaatg cgtgatcgtg caccatcaac acgttatcga
     2221 atcctttgcc gcgcaaatcc gcatcttcat gacgaccaaa gccagtaaag tagaacggtt
     2281 tgtggttgat caggaactgt tcgcccttca ctgccactga ccggataccg acgcgcagcg
     2341 ggtagatatc acactctgtc tggcttttgg ctgtgacgca cagttcatag agataacctt
     2401 cacccggttg ccagaggtgc ggattcacta cttgcaaagt cccgctggtg ccttgtccag
     2461 ttgcaaccac ctgttgatcc gcatcacgca gttcaacgct gacatcacca tttgccctca
     2521 cctgccagtc aacagatgcg tggttacagt cttgcgcgac atgcgtcacc acggtgatat
     2581 cgtccaccca ggtattcggc gtggtgtaga gcattacgct gcgatggatc ccggcgtagt
     2641 taaagaaatc atgaaagtaa gactgctttt tcttgccgtt ttcgtcggta atcaccattc
     2701 ccggcgggat agtctgccag ttcagttcgt tgttcacaca aacagtgata cgtacacttt
     2761 tcccggcaat aacatacgga gtgacatcgg cttcaaatgg cgtatagccg ccctgatgct
     2821 ccatcacttc ctgattattt acccacactt tgccgtaatg agtgaccgca tcgaaacgca
     2881 gtacaatacg ctggcccgcc caacctttcg gtataaagac ttcgcgctga taccagacgt
     2941 tgcccacata attacgaata tctgcatcgg cgaactgatc gttaaaactg cccggcacag
     3001 caattgcccg gctttcttgt aacgcgcttt cccaccaacg ctgatcaatt ccacagtttt
     3061 cgcgatccag actgaatgcc cacaggccgt cgagtttttt gatttcacgg gttggggttt
     3121 ctacaggacg taacataagg gactcctcat taagataaaa atactggtca acctttaatc
     3181 tgattagtta accaattctg tgttgcacac agttatctgt gagaaaccca tggaagagaa
     3241 atggaaataa aaagaactga caccgcgatc aaaaaaccaa aaactgcaat acccacagcg
     3301 tgatattgat cgcattaatc tttaaaacag cgttgacaga agccacaaaa taagcaatcg
     3361 aacgttcgtt cgaacatatt gggagcgata aaccctcatg atatattgct acgatgaata
     3421 tttaggtggg attctccgat ccctgggagg atgatttttg cacagagaaa ttacgatatg
     3481 aaacgcgggc tcagttgtca ccagcccgcg gaaagagaga gaggatcagg atgcggttaa
     3541 tataccgcca atcatcgcgc gtaatccctg agcaaacgac gcttcacgcg ccagatcttc
     3601 cgcttcgata tcggccagta caccgtaggt cagatccagc agtaaacgtg aagtcattgc
     3661 cgtgttgata tctgggctta tctcgccttt ttgctgggca tcacgcatcc gctgagcaac
     3721 aaattccgtg atcgtcatat gcttattttt aagcatggcg gcgacctgtg ggttacgcat
     3781 cccttccgcc ataatttcga ccaccagcgc ccgttgccca aaggcctcat gggtgagaga
     3841 gacaatagac tcgaccatat agtcaacgaa atgaatccct tcaatcggtt cccggaaacg
     3901 ggccagcgcc ctctcctggt cctgtaagat aatcgcctga atcaaggctt ctttggagat
     3961 gaaatggtga tagagcgtcc cgggactaat ggcgcaggat ttacagatgg ctttcatcga
     4021 ggcactgtga aatccatttt ctgaaaaaat ctctctggca gcattgagga tccgggtccg
     4081 tgtcggttgt gcttcagtct gcatgttatc catcattaaa ccattatagt tagcatgaat
     4141 aactagttta ccgcgttgtc aggtgagcgc ctatcattgc tgttgaatag tgcgaaggca
     4201 cactcaattc gtaagctttt tatccagata ttggttaacc aatagccgtg atttggctgc
     4261 cagttgttta gcagagacgt tgatgagttg cacacgctat cccggtaaac aacacatacg
     4321 gctgtttacc ggttcgttag tgtattaatt gagctcctgt accccaccac cggagacggt
     4381 gagaatttgt ccgcttaccc agctggctgc aggcgagcaa aggaacagcg ccgcgttagc
     4441 tatatcttgc ggttggccca gacgtctgat tggtgtgtgt tgcaacattt tctgttcaat
     4501 ttctggtgta ataacggatt tcagggcatc ggttaatatt gcccccggtg caatgccatt
     4561 cacccgaata tttttttcac ccaggtcaaa cgccatattt ctgaccagat gactggccgc
     4621 agctttagat gatgcatagg aggtcatgtt tatattttta ttttctgccg ccatagaagt
     4681 aatggtcaga ataacgccac cgccattttt ttccatttct ggcgcaacaa gttgtgacag
     4741 atggaaaaaa gaaaatacat tcagttcata agcgcgacga aaatctgcca ttggcatatc
     4801 aaacggttta ggcccaccgc caccggcgtt gttaaccagg atatcaactt tacccaactt
     4861 actgacggca aagtctgcca gtgcagagag ttcctgctcg gaagtaatat cgcagcggca
     4921 ggcaaatgcc tgaccaccca gttgttgaat ttcatctaca acatgattag ctgcatcggc
     4981 gttaatatca ctgaccacca cagatgcgcc agctgtcgcg aatgtaatgg cgatttcttt
     5041 accaatacct gcacccgcac ctgtaatgat ggcgcatttt ccgtcgagtc tcaggttgtc
     5101 agaattaaac acataaacct cctgtgaact tcagtaaaaa taaaaggcac cgcctgtgtg
     5161 ccacattttt tatcattaac cttgaggaaa ggacactacc aggtatagca gataaagaga
     5221 ggattatttc gccgcaatga gccgggcggg aataataaga ttgcgtgaat gcgtctcttc
     5281 atgggtgatt ttttgcatca tgcgatccgc aagtgtggta ccaagttccc gcgctggcgt
     5341 gctggcccag gtaacgggta tatcatcaag tgtggttggt gtcgcatcgg taaatgccgc
     5401 cagcgaaacc tgttgctcaa agtaacgatc gactccgctt tccccgcttt gcctccctgc
     5461 tttcagcaaa ccaaaccatg cccccatcgc aatagtttcg ttatagcaaa ccacggcgct
     5521 gatggtcggg ttatgacgta aaagcgccgt gatagcttcc gcggcttgct tctggctgga
     5581 agtgcactcc agcacccaat cgctgtgaaa cggcaggcca aattttagta gagtcgcaca
     5641 atagcccccc acccgttctg cccgggttaa tgaggaactt tgccctccca gccaggcgat
     5701 acgctgatgc ccattgcgaa tgagatgctc cgtcaacaac tgtgcagcct gcatgttgtc
     5761 cgggcgaacc gtatcaacgt catcaagata actggcacgg gaagcgaaaa tcaccgggat
     5821 agctttttct tctgctatcc gtcgcaggtc atcgctgctt cctgcagccc cggcaatgac
     5881 cacaccatcg acaccctgat tcagtaatag tgaaaaccgc tgtgccagct gctcaccgtc
     5941 tttaccgccg tgaagcaaaa aaaccatccg tccctgcgct tccagagctt ccgtcaatcc
     6001 ggccgtcaat tcggcgtaaa acggcgcaga taaatcacgg acgatcaaac caatgacgcc
     6061 gctttgcccg ccgcgcagcg ccgacgcctg acgattgcgc acaaatccca gctcttcaat
     6121 ggcggcgtta acgcgttctc ctgtggcggt agagattcgc cctttgccac ttagcaccag
     6181 cgaaacggta cttaccgaca cgcccgcagc cagcgcaaca tcatgaatgg ttattttttt
     6241 ggcggtagcc atgcagatga cctactccct gattatgtga cagataaaac gttttacctt
     6301 ttatttatct tatacccgct attatcgttg cgtaatgtga tttatacctc actaaaattt
     6361 gataaaacgt tttatcttct cgcgcaattt actgaatcca gattgttctc tacgaggagt
     6421 cgttttatga cggcgaaaac agcaccgaga gtcacgctgt gggagttctt ccagcagtta
     6481 ggcaaaacct tcatgttacc cgtggcatta ttgtcgttct gcggcattat gctcggcatt
     6541 ggtagttctc ttagcagtca tgatgtcatc accctgatcc cggtcctggg caaccccgtg
     6601 ttgcaggcta tctttacctg gatgagtaag attggctcgt ttgcttttag tttcctgcca
     6661 gtcatgttct gtatcgccat cccgctgggt ctggcacgcg aaaacaaagg cgtagcggca
     6721 ttcgctggct tcgtcggtta tgcggtaatg aacctcgcgg taaacttctg gttgaccaat
     6781 aaaggcattc tgccaaccac ggacgccgcg gttctgaaag ccaataacat ccagagcatt
     6841 cttgggatcc agtcgatcga caccgggatc ctcggtgcgg tgatcgccgg tattatcgtc
     6901 tggatgctgc atgagcgatt ccataatatc cgcctgccag atgcgctggc attcttcggc
     6961 ggtacgcgct tcgtaccaat catctcctcg ctggtgatgg gtcttgtcgg cctggtgatt
     7021 ccattagtct ggccaatttt cgccatgggt attagcggct taggccatat gatcaacagc
     7081 gcgggtgatt tcggaccgat gctgtttggt accggtgaac gtctgctgtt gccgtttggt
     7141 ctgcatcaca ttctggtggc attaattcgc tttaccgacg caggcggcac gcaggaagtc
     7201 tgcggtcaaa ccgtcagcgg tgcactgacc atcttccagg cgcaattgag ttgcccgacc
     7261 actcacggtt tttctgaaag cgccacgcgt ttcctttcgc aaggcaaaat gcctgcgttt
     7321 ctcggcggtc tgccaggtgc agcgttagct atgtatcact gcgcgcgccc ggaaaatcgc
     7381 cataaaatta aaggtctgct gatttctggc ctgatcgcct gtgtcgttgg cggcacgacc
     7441 gaaccgctgg aattcctttt cctgttcgta gcgccagttc tgtatgtcat ccacgcgctg
     7501 ttaaccggcc tcggcttcac tgtcatgtct gtgctcggcg tcaccatcgg taataccgac
     7561 ggcaatatca tcgactttgt ggtgttcggt attttgcatg gtctgtcaac caagtggtac
     7621 atggtgccag tagtggcggc aatctggttt gtcgtttact acatcatctt ccgtttcgct
     7681 atcacccgct tcaatctcaa aaccccgggg cgcgatagtg aagttgccag ttcaatcgaa
     7741 aaagccgttg ccggtgcgcc gggtaaatca ggttacaacg ttccggcaat cctcgaagcc
     7801 ttaggcggtg ccgacaatat tgtcagcctc gataactgca ttacgcgtct gcgtttgtct
     7861 gtaaaagata tgtcgcttgt taatgtgcag gcactgaagg acaatcgggc aattggcgta
     7921 gtacaactta atcaacataa cctgcaggtt gttatcgggc cacaagttca gtcagtaaaa
     7981 gatgaaatgg ccggtctgat gcatactgtc caggcataag gataagatat gttcgatttt
     8041 tcaaaggtcg tggatcgtca tggcacatgg tgtacgcagt gggattatgt cgctgaccgt
     8101 ttcggcactg ctgacctgtt accgttcacg atttcagaca tggattttgc cactgccccc
     8161 tgcattatcg aggcgctgaa tcagcgcctg atacacggcg tgtttggcta cagccgctgg
     8221 aaaaacgatg agtttctcgc ggctattgcc cactggtttt ccacccagca ttacaccgcc
     8281 atcgatcctc agtcggtggt gtatggccct tctgttatct atatggtttc agaactgatt
     8341 cgtcagtggt ctgaaacagg tgaaggcgtg gtgatccaca cacccgccta tgacgcattt
     8401 tacaaggcca ttgaaggtaa ccagcgcaca gtaatgcccg ttgctttaga gaagcagcct
     8461 gacggctggt tttgcgatat gggcaagctg gaagccgtgt tggcgaaacc agaatgtaaa
     8521 attctgctcc tgtgtagccc acagaatcct accgggaaag tgtggacgtg cgatgagctg
     8581 gagatcatgg ctgacctgtg cgagcgtcat ggtgtgcggg ttatttccga tgaaatccat
     8641 atggatatgg tttggggcga gcagccgcat attccctgga gtaatgtggc gcgcggagac
     8701 tgggcgttgc taacgtcggg atcaaaaagt ttcaatattc ccgccctgac cggtgcttac
     8761 gggattatag aaaatagcag tagccgcgat gcctatgtat cggcactgaa aggacgtgat
     8821 gggctttctt ccccttcggt actggcgtta actgctcata tcgccgccta tcagcaaggc
     8881 gcgccgtggc tggatgcctt acgcgtctat ctgaaagata acctgacgta tatcgcagtt
     8941 aaaatgaacg ctgcgtttcc tgaactcaac tggcagatcc cgcaatccac ttatctggcc
     9001 tggcttgatc tacgtccgtt gaatattgac gacaacgcgt tgcaaaaagc gcttatcgag
     9061 caagaaaagg tcgcgatcat gccgggatat acctacggtg aagaaggtcg tggttttgtc
     9121 cgactcaatg ccggctgccc acgttcgaaa ctggaaaaag gtgtggctgg attaattaac
     9181 gccatccgcg ctgttcgtta accccaattg cgcaacgtaa aaaatcgttg cgcaatcgtg
     9241 gatttttacc ctgctttgtt tttataatgg tgcgcacttt tatatccaga aaaagagtgc
     9301 gaccatgatt gataccaccc tgccattaac tgatatccat cgccaccttg atggcaacat
     9361 tcgtccccag accattcttg aacttggccg ccaatataat atctcgcttc ctgcacaatc
     9421 cctggaaaca ctgattcccc acgttcaggt cattgccaac gaacccgatc tggtgagctt
     9481 tctgactaaa cttgactggg gcgttaaagt tctcgcctct cttgatgcct gccgccgcgt
     9541 ggcatttgaa aacattgaag atgcagcccg taacggcctg cactatgtcg agctgcgttt
     9601 ttcaccaggc tacatggcaa tggcacatca gctgcctgta tcgggtgttg tcgaagcggt
     9661 gatcgatggc gtacgtgaag gttgccgcac ctttggtgtg caggcgaagc ttatcggtat
     9721 tatgagccgg accttcggcg aagccgcctg tcagcaagag ctggaggcct ttttagccca
     9781 ccgtgaccag attaccgcac ttgatttagc cggtgatgaa cttggtttcc cgggaagtct
     9841 gttcctttct catttcaacc gcgcgcgtga ttcgggctgg catattaccg tccatgcagg
     9901 cgaagctgcc ggaccggaaa gcatctggca ggcgattcgt gaactggggg cggagcgtat
     9961 tggacatggc gtaaaagcca ttgaagatcg ggcgctgatg gattttctcg ccgagcaaca
    10021 aattggtatt gaatcctgtc tgacctccaa tattcagacc agcaccgtgg cggatctggc
    10081 tgcacatccg ctgaaaacgt tccttgagca tggcattcgt gccagcatta acactgacga
    10141 tccaggcgtg cagggagtgg atatcattca cgaatatacc gttgccgcgc cagctgctgg
    10201 gttatcccgc gagcaaatcc gccaggcaca gattaatggt ctggaaatgg ctttcctcag
    10261 cgcagaggaa aaacgcgcac tgcgagaaaa agtcgccgcg aagtaacaaa aatggatggt
    10321 gctaattgca ccatcccctt ttcacgcaag gcacaaagtc gcgcgatgtt tggcggattc
    10381 gatgcccagc tcaatcaact ccattacctg gattgcctga cttgccggaa ccggattttc
    10441 accatcgcca tttaacgcat cacgaatagc cgcatagtaa gccggataat tccccggcac
    10501 tgtcaacagc gtttcttcga cacgttcctc accttccacg cgggtaagta cgccatcgcg
    10561 catatcgtag ccccagtctt cctgcggcag acgctcgcca tttttcagac gttcttcctg
    10621 tggatcgagg ccatatttca cataactgcc tcgggatcca tgcacgatat accgtgctga
    10681 ctcggctgct gccagcatgg tgccgtgtaa aatcactcgc cgctgcggat aggacaagat
    10741 agcgtggaaa taatcggtcg actgcgctcc gggccgtaac tgtgccaaat ctaccgtcat
    10801 gctaaccggt aaaccaaata acgtaatggc ctgatcaaga aggtgcggtg ctaaatcgta
    10861 ccagataccg ctgcctgggc cgccctgttc acgccaacga tcgcgcacct gcgggcggaa
    10921 gcggtcaaaa tgggactcaa agtaagcaat ttcacccagc acgccttccg cgagcaaacc
    10981 ttttagcgtc aggaaatcgc tatcccagcg acggttatgg aatacagaca gcactcgccc
    11041 caagcttttt gccagcgctt ccagctctcg tgcttgtgac agtgtcacgg taaagggttt
    11101 atcaacgacc acatgtttac ccgcctcaag cgccgctttg gctaacggaa aatgggtatc
    11161 gttgggtgta ggaatgacaa tcaggtctat gttgggatcg ttaaacagat gcttcggctc
    11221 agagacaacc gtaaccgttg gccagtcagc ttttactttt gtttcatcgc tgctggagat
    11281 taccgccagc tccagcccgg gcgtgcccgc aatcaggggt gcatggaagg ttttgctcgc
    11341 ataaccatac ccaatcaacc caacacggat gttgtcgctc atgtcattgc ctctcattga
    11401 agtatgatgg ctatttgaca ctatccttta ccctcgctca acagtttaat aacctgccag
    11461 caataaggga tgttgtttaa cttaagtcaa aaaaatagcg atttttccaa cgacaaaagc
    11521 taaatattgc aaaaacctca gtaaaaatct tgctggagct attattgcta agtaacattt
    11581 accccctgaa gttaatggat caatcaacag agatgtgggc tgtaatgaat cgtcttattg
    11641 aattaacagg ttggatcgtt cttgtcgttt cagtcattct tcttggcgtg gcgagtcaca
    11701 ttgacaacta tcagccacct gaacagagta ctacagtaca acacaagtaa gctctgcact
    11761 tgttgagcga catgctgccc gtccgggtgc atgttttcac ttgtcggata ttaaatcagg
    11821 aatttattat cttgttcgat gttgttgatg attgtcaggg atagtaaagc gcgttactct
    11881 ttccagagca gtttttcatc cctgaatatt tgccatcagc acattcttat tattgtaata
    11941 acacaagcgt tcacttttgg tgaaatttta tcatcactaa taatacctac ccctttggta
    12001 ttaattattt tatggacctt ttatgactgt tcaggactac ttattaaaat ttcgcaaaat
    12061 cagttcactc gaaagtctgg aaaaactcta cgaccatctt aattacaccc tgacggacga
    12121 tcaggaactg atcaatatgt atcgtgctgc cgatcaccgt cgcgcagagc tggtttctgg
    12181 cgggcgtttg tttgacctcg gccaggtacc gaagtccgtc tggcactatg tccagtaaag
    12241 aaagtagcga tcggttataa aagccttata ataacgcccc taaaattaga tttgtctgtt
    12301 tgcgtcgcta ttctggagaa cctatgacca ccacaacgcc acaacgtatt ggaggctggt
    12361 tgcttggccc gttggcctgg cttttagtcg ctctgttgag tacgacgctg gcgttgttgc
    12421 tgtataccgc tgcgttatct tctccgcaaa catttcaaac gctcggcgga caagcactga
    12481 cgacacaaat cttatggggc gtttctttta ttaccgctat cgccatgtgg tattacacac
    12541 tgtggttgac catcgctttc tttaaacgtc ggcgttgtgt tcctaaacac tatattatct
    12601 ggctgcttat ttccgtattg ctggcagtaa aagcgttcgc tttttcaccc gtagaagatg
    12661 gcatagccgt tcgccagttg ctgtttactt tgttggcaac cgcattgatc gtcccctatt
    12721 tcaaacgctc gtcgcgggtg aaagcgacgt ttgtgaatcc gtaataacct tacagttaac
    12781 ctgttgtcgc ctgctctgga ttaacggata ataggcggct tttttatttc aggccgaaaa
    12841 atgactgact acctgttact gtttgtcgga actgtactgg tcaataactt tgtactggtc
    12901 aagtttctcg gtctctgtcc gtttatgggg gtttccaaaa agctggaaac cgcgatgggc
    12961 atggggctgg caacaacgtt tgtaatgacg ctggcgtcta tttgcgcctg gcttatcgat
    13021 acgtggattt tgatcccact taatctgatt tacctgcgca ccctggcgtt tattctggtg
    13081 attgctgtgg ttgtgcagtt caccgaaatg gtggtgcgca aaaccagccc ggtactttac
    13141 cgtctgctgg ggattttttt gccgcttatc accaccaact gtgccgtgct cggcgtggcg
    13201 ttgctgaata tcaatctcgg gcacaatttc ttgcagtcag cgctgtacgg tttttccgcc
    13261 gctgtcggtt tctcgctggt gatggtgctc ttcgccgcca tccgcgaacg ccttgctgtg
    13321 gctgatgtcc cggcaccttt tcgcggtaat gccattgcgt taattaccgc aggtcttatg
    13381 tctctggcct ttatgggctt tagtggtttg gtgaagttgt aatgaatgct atctggattg
    13441 ccgttgccgc cgtgagcctg ctgggcctgg cgtttggcgc cattctgggt tatgcctccc
    13501 gccgttttgc ggtggaagac gatccggtcg ttgagaaaat tgacgaaatc ttaccgcaga
    13561 gccagtgtgg tcagtgcggt tatcccggct gtcgccccta cgcggaagcc atcagctgta
    13621 acggtgaaaa aatcaaccgt tgcgccccag gtggcgaagc tgtgatgcta aaaattgccg
    13681 agttgcttaa tgtcgagccg cagccgctgg atggcgaagc gccagagcta acgccagcgc
    13741 ggatggtggc ggttattgat gaaaataact gtattggctg cactaaatgt attcaggcgt
    13801 gtccggtaga cgccatcgtt ggcgctaccc gcgccatgca tacggtaatg agtgatctct
    13861 gtacgggctg caatttatgt gttgatccgt gcccgacgca ctgcatctcg ttgcaaccgg
    13921 tcgcagaaac tcctgactcc tggaaatggg atctgaacac cattcccgtg cgtatcattc
    13981 ccgtggaaca ccatgcttaa gttattctct gcattcagaa aaaataaaat ctgggatttc
    14041 aacggcggca tccatccacc ggagatgaaa acccagtcca acggtacgcc cctgcgccag
    14101 gtaccactgg cgcagcgttt tgtcattcca ctgaaacaac atattggcgc tgaaggtgag
    14161 ttgtgcgtta gcgtcggcga taaagtattg cgcggccagc cgcttacccg tggtcgcggc
    14221 aaaatgctgc cggttcacgc gcccacctcg ggtaccgtta cggctattgc gccccactct
    14281 acggctcatc cttcagcttt agctgaatta agtgtgatta ttgatgccga tggcgaagac
    14341 tgctggatcc cgcgcgacgg ctgggccgat tatcgctctc gcagtcgcga agagttaatc
    14401 gagcgcattc atcagtttgg tgttgccggg ctgggtggtg cgggatttcc gacaggcgtt
    14461 aaattgcagg gcggcggaga taagattgaa acgttgatta tcaacgctgc tgagtgcgag
    14521 ccgtacatta ccgccgatga ccgtttgatg caggattgtg cggctcaggt cgtagagggt
    14581 atccgcatcc ttgcgcatat tctgcagcca cgcgaaattc ttatcggtat tgaagataac
    14641 aaaccgcagg cgatttccat gctgcgcgcg gtgctggcag actctcacga tatttctctg
    14701 cgggtgattc ccaccaaata tccttctggc ggtgctaaac aattaaccta cattctgacc
    14761 gggaagcagg ttccacatgg cgggcgttca tccgatatcg gcgtattaat gcaaaacgtc
    14821 ggcaccgctt atgcagtgaa acgtgccgtt attgatggcg agccgattac cgagcgtgtc
    14881 gtgaccctga ctggcgaagc aatcgctcgc ccgggcaacg tctgggcacg gttggggacg
    14941 ccagtgcgtc atttattgaa tgacgccgga ttctgcccct ctgccgacca aatggtgatt
    15001 atgggcggcc cgctaatggg ctttaccttg ccgtggctgg atgtcccggt cgtaaagatt
    15061 accaactgtc tgttggctcc ctctgccaat gaacttggcg aaccacagga agaacaaagt
    15121 tgcatccgtt gtagcgcctg tgctgacgcc tgccctgcgg atcttttgcc gcaacagctg
    15181 tactggttca gcaaaggtca gcaacacgat aaagccacca cgcataacat tgccgactgc
    15241 attgaatgtg gagcttgcgc gtgggtttgc ccgagcaata tccccctggt gcaatatttc
    15301 cgtcaggaaa aagctgaaat tgcggctatt cgtcaggaag aaaagcgcgc cgcagaagcc
    15361 aaagcgcgtt tcgaagcgcg ccaggctcgt ctggagcgcg aaaaagcggc tcgccttgaa
    15421 cgacataaga gcgcagccgt tcaacctgca gccaaagata aagatgcgat tgctgccgcc
    15481 ctggcgcggg tgaaagagaa acaggcccag gctacacagc ctattgtgat taaagcgggc
    15541 gaacgcccgg ataacagtgc aattattgca gcacgggaag cccgtaaagc gcaagccaga
    15601 gcgaaacagg cagaactgca gcaaactaac gacgctgcaa ccgttgctga tccacgtaaa
    15661 actgccgttg aagcggctat tgcccgcgcc aaagcacgca agctggaaca gcaacaagcc
    15721 aatgcggagc cagaacaaca ggtcgatccg cgcaaagccg ccgtcgaagc cgctatcgcc
    15781 cgtgccaaag cacgcaagct ggaacagcaa caggccaatg cggagccaga agaacagatt
    15841 gatccgcgca aagccgccgt cgaagcggct attgcccgtg ccaaagcacg caagctggaa
    15901 cagcaacagg ct
//
LOCUS       DADSYM010000113        15882 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2415
            SAMN08039579-rid11723173.denovo.114, whole genome shotgun sequence.
ACCESSION   DADSYM010000113 DADSYM010000000
VERSION     DADSYM010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039579
            Sequence Read Archive: SRR6362533
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15882)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15882)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980162.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 03:39:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,484
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 4,347
            CDSs (with protein)               :: 4,347
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 43 of 85
            Pseudo Genes (incomplete)         :: 37 of 85
            Pseudo Genes (internal stop)      :: 19 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15882
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039579-rid11723173.denovo.114"
                     /strain="LGE2415"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8302 N 95.9628 W"
                     /collection_date="2015-08-24"
     gene            177..791
                     /locus_tag="J1Y83_003172"
     CDS             177..791
                     /locus_tag="J1Y83_003172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000772910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus Gp2 family protein"
                     /protein_id="HBA7631297.1"
                     /translation="MKLNERYGKPNAHYMKRLEEITRYAMDDYARILAFRVDLLLPVD
                     NSHKGYDSAVITRFIASLKAQIAAYLQRRRRDGKRVYPCKLRYAWVREFGELNGNKHY
                     HMLLLVNREVFRNTMLNTENSAQPRGLLVGMVARAWAVALRRTGDMANKVPFHLPGVS
                     YELDRRNGTNTDFFRSYIYHISYLAKEYTKNHDDGERNFGCSQG"
     gene            909..1127
                     /locus_tag="J1Y83_003173"
     CDS             909..1127
                     /locus_tag="J1Y83_003173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001064742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AlpA family phage regulatory protein"
                     /protein_id="HBA7631298.1"
                     /translation="MNNQPNTDTDLMNDKLVTMAFITTFTGLTDKWFYKLISEGKFPK
                     PIKLGRSSRWRESEVKRWLTERIAESRY"
     gene            complement(1347..2132)
                     /locus_tag="J1Y83_003174"
     CDS             complement(1347..2132)
                     /locus_tag="J1Y83_003174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000766854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA7631299.1"
                     /translation="MKLIIIPALFVSLFAFHPAYAVGTYDLQCMLDNGEQMTLSHVSN
                     TVYISFETPGGDSEESGSVIKLDIPSGEAKQTLAANPGAGTASFTLHGENEDIEGAVA
                     VNYSEYDGTGDAYYTAMNSMGQETSTVSCKPGTIKVSRSLLQNGINGVGSQQANKPDP
                     SQQQQAQQSTTPPFKVQFGSSVSNEGWNTRYGVIQLTITDDNVVLKSIRVNRGNCKME
                     SVGNRTLPAKYKFGDVATFKYMKCDRIIEADIVTDTGSWTFNS"
     gene            complement(2144..3157)
                     /locus_tag="J1Y83_003175"
     CDS             complement(2144..3157)
                     /locus_tag="J1Y83_003175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001683509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase"
                     /protein_id="HBA7631300.1"
                     /translation="MDDPVKGEGRMITDASGVTRREGGILGMWGNIFPERNGSNEQKG
                     DSTPHIPDIRDILDIFPQPLREKLLNKISEAIDYEPVIGVMGKTGAGKSSVCNALFKG
                     EVCAVSDVEACTREVQELRIRFGKRSLKIIDIPGVGENARRDKEYEDLYRNLLPSLDL
                     ILWVIKGDDRAFSADEHFYNNVLLPAGGGERVLFILNQVDKIEPFREWDTHLHQPSPA
                     QRINIEKKEAYITERFGFTHYPVTPVSADEGYNILRLVETIIRALPDRAKSSTASQFK
                     DEFKTEEVKTEAKNGFGSVISGILDDVIDSFPLPKPVKELTRKGKDKLIEWAGNAWDH
                     FFG"
     gene            complement(3196..3912)
                     /locus_tag="J1Y83_003176"
     CDS             complement(3196..3912)
                     /locus_tag="J1Y83_003176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001383792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WYL domain-containing protein"
                     /protein_id="HBA7631301.1"
                     /translation="MIFWENRTVPTMKTQHDHHDRLSVRLSVIISRLLAGESLELKVL
                     ADEFGVSERTLQRDFQQRLIHLDLEHRSGSYRLIRQTGREHLPNTFAFIRNSGISGII
                     PAQNRKLIWLIASNSGVSPCLIGHGALSSPVTQTACFLELAEAIRDYRAVTLLVNGKS
                     YEAVAPRRLIFQSKNWFLVATHTRNLQVFRMEDVNAVTVLDTSFRRKPELDALTASEE
                     FISALPHFRFISDVIQTFRE"
     gene            4550..5092
                     /locus_tag="J1Y83_003177"
     CDS             4550..5092
                     /locus_tag="J1Y83_003177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000338492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA7631302.1"
                     /translation="MDAEKIQSDIDALLTYMNDDMENLGETIKTVTFGFETGQNDRIA
                     IDELLHWREEYLFKIINICHSRSLLRKDYGDFEHVSLTEEGQSRAISIKHGKNRSYEL
                     GASMQIATLNVHGPAQVGNSNTQNIHNVFNQLLQQIEKSSASDEEKQEAKKRLAKFLE
                     HPLVSSIVGGLAGSLPGLMG"
     gene            5391..6365
                     /locus_tag="J1Y83_003178"
     CDS             5391..6365
                     /locus_tag="J1Y83_003178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001552312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA7631303.1"
                     /translation="MSRTKVLFAYCHELDRCLSIDEARTEFFSKKPQDRQRFTFSCSD
                     RNCNVTISGVNYHVKAEDGEKFKVAHFRSPHPHNLGCEWMQFSEEANQPRQASETEAD
                     YTERKARQKLNDFINCFDPCTDETDSGQKKTGQLTSLSQVNHLVKDNDLSNDKSHKWS
                     RYTRTNQLQRLVDTWQEAKKTLTYDEFRTLKLRVTNYGKVYFHEYITHIQKGLKNQYE
                     GVIFGGGSLVKRYGRGFLIHFFDKHNDKPIRLYIDKNLMEKGRFSHYIDEILSTENVR
                     YFRVFLLNPEVSEKKDRTGKPVINLEISSLRQLAIYYELNSSEDNDFV"
     gene            6403..7056
                     /locus_tag="J1Y83_003179"
     CDS             6403..7056
                     /locus_tag="J1Y83_003179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015227171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HBA7631304.1"
                     /translation="MAPGYVYILINPSMPGLIKIGRTLRDSRMRARELFSTGVPTPFQ
                     VAFELFAEQHEALEATVHNELNDFRVNVAREFFRYPLDKAVALLIQLPPLPNSTEQYV
                     AEDITQRLREKYPLYIRPEIVAVRIVQMPGGVWLKITTEKEIAGYLVDQIIQRTDLAF
                     IVDTDEAFFRSEDDVRLNANKLVNDYDIYSIVMTTDLFHDYACQQITRAHHAQRNFV"
     gene            complement(7087..7976)
                     /locus_tag="J1Y83_003180"
                     /pseudo
     CDS             complement(7087..7976)
                     /locus_tag="J1Y83_003180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418771.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
     gene            complement(8135..8293)
                     /locus_tag="J1Y83_003181"
                     /pseudo
     CDS             complement(8135..8293)
                     /locus_tag="J1Y83_003181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009310009.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
     gene            complement(8437..11352)
                     /locus_tag="J1Y83_003182"
     CDS             complement(8437..11352)
                     /locus_tag="J1Y83_003182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020324812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HBA7631305.1"
                     /translation="MATQSEYELETQLVEQLVGMHYERVNVTDETSMKANLRKQIEIH
                     NRLESSPLTDGEFNRIFLHLTKGNEVIDRAKILRDRYQLLRDDGTEKWLSFINKEEWC
                     QNEFQVTNQVKQFNAEQNTRKTRFDVTLLINGLPLVQIELKRRGLGLKEAFNQIDRYH
                     RDAFWVGSGLFQYVQIFIISNGVDTKYYANNRANHFEQTFFWTDEKNNPLKALEKFTD
                     AFLKVCHIAKMITHYTVLNETRKCLMVMRPYQFYACEAMIRHVKENLHKQGRPENGYI
                     WHTTGSGKTLTSFKASQVIMEMPEVDRVIFVVDRRDLDYQTAKEFNGFAKDSVDTTNN
                     TSTLIKHLKQSSTKLTLTTIQKLNNAITHEGYKAQLAHLRKERIVFIFDECHRSQFGD
                     THKAIVNFFENAQLFGFTGTPIFADNVNITNGVKQTTELLFDKCLHKYVIVDAIRDEN
                     VLRFAVEYVGTYKRKESSNEIDIDVEDIDTKEVMESDIRLGKVTDYILAHHNAKTRNR
                     EFTAMFCVGSVDMLITYYKLFKEKQAALQAADANYKPLKIATIFTYAANEADKSDKEA
                     VNGLLDEEDISLPQGSKVDTSSRDHLDSFIKDYNELYGTSYSANDSKSFYNYYKNIAQ
                     RTKEKGIDILLVVNMFLTGFDSPALNTLYVDKNLRFHGLVQAFSRTNRILNEKKSHGN
                     IVCFRNLKKATDDAIALFSNKDASSVVLVKPFDEYFGDYQDAVDRLLTLTPTVDSVDD
                     LPDEEAQLAFVTAFRDVMRLKNILESFADFDDAAKPLSDQTLADFTSKYLDIHDKVKK
                     HSSKEKVSILDDVDFEVSLIHRDEINVGYILRLLAGIQAMPLKEQQEQKKKIMDIVDS
                     DVTLRSKRELIDNFIEKSLLHASHDEDITQVFEAYVEQEKDKALATLCSEERLDSIKL
                     AGLLDNYLFTDVMPREDEVAETLTWTPKILERKSVRARVYERVRDVIDTFINGMRGF"
     gene            complement(11411..12565)
                     /locus_tag="J1Y83_003183"
     CDS             complement(11411..12565)
                     /locus_tag="J1Y83_003183"
                     /inference="COORDINATES: protein motif:HMM:NF013579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA7631306.1"
                     /translation="MMVPKLRFYRFLNNDEYKWQSKKITDISKTFIGLVTTMTSSYVD
                     NGVKLIRNSDIKENYVEKSKLIKLDISFAEQYGSRKLKSGDVVTVHTGDIGVSAVIDE
                     ELDGCLGFATLNTRPNPTIINSKYLCQYFNSPYYIKYALSMATGDGRNNYNLKDFNNA
                     IIPVPCIDEQTKIADFLFSVDEKIALLNKQYNLLSQYKKGMMQKIFSQELLFKDENGE
                     EFSEWNRKFIGNILTIGSGRDYKHLNAGNVPVFGTGGFMTMVDQYLFEGETVCIGRKG
                     TIDKPFYYNGKIWTVDTLFYTHEFKGAHPKFVYQLFLTINWKVYNEASGVPSLSKNTI
                     EKIEVNIPCLSEQNKISSFLSTLDDKIAAKKAELDKLKTWKQGLLQQMFV"
     gene            complement(12562..14169)
                     /locus_tag="J1Y83_003184"
     CDS             complement(12562..14169)
                     /locus_tag="J1Y83_003184"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013364708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HBA7631307.1"
                     /translation="MSPQIEAQNLSELQGRLWNIADTLRGKMNADEFRDYCLGFIFYK
                     YLSEKFVAYANKILAEDDIQYNELTVEHPAYQDIVDAVKEDSIQTLGYFLPPTDLFHT
                     MAERVAKDPKGTGTGFILEDLATTLRSIEQSTLGTDSADDFSNLFEDLDLGSSKLGNT
                     AKAKNELIGKVVTELDKLSFNLSEASSDILGDAYEYLIGQFASGAGKKAGEFYTPQPV
                     STLLAKIVTTHKLKLKNVYDPTCGSGSLLLRVKREASSVGKIYGQEMNRTTYNLARMN
                     MILHGVHYADFEIIQEDTLEHPQHTHLKFDAIVANPPFSAKWSASPLFMNDDRFAQYG
                     KLAPSSKADMAFLQHMFHHLEDDGTMAVVLPHGVLFRGAAEGHIRQFMIEKLNCIDAV
                     IGLPANIFYGTSIPTCVLVLRKCRKHNDSILFIDASNDFEKVKTQNRLLPEHIDKIAD
                     TYNDWKAIEKYSHIATLEEIRNNDYNLNIPRYVDTFEAEEEIDLNAVAQEIRDLEGKV
                     AEIDKTIAGFCKELGIDAPFALAEENK"
     gene            complement(14189..14764)
                     /locus_tag="J1Y83_003185"
     CDS             complement(14189..14764)
                     /locus_tag="J1Y83_003185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HBA7631308.1"
                     /translation="MGKIVYSDIANFIPGVNLSRIEPEESGKVYRFYTAQLHQIDDWQ
                     PEVIVTDKEQVVKSTSLLKVTRENDVIINLANAKAVLVKQDHAGRILGNNFVKVELDV
                     SRVSPLFWIWHFNESKAVAQQKSSALQGSTVPKLTLQMVRQFELDLPGLKKQQAIGEL
                     YLHLRMKDYYEYNLMKLKQQYYFAKIENLSK"
     gene            complement(14811..15821)
                     /locus_tag="J1Y83_003186"
     CDS             complement(14811..15821)
                     /locus_tag="J1Y83_003186"
                     /inference="COORDINATES: protein motif:HMM:NF027588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4917 family protein"
                     /protein_id="HBA7631309.1"
                     /translation="MVTIKQWAEIEAAYNDTLLLGNGASIAVNSDFAYRSLCQKLTEL
                     SSDGQLTALFELFDTQDFELILHRLWIATQVNQQLGVPENATTEGYKLLRNLLIQCVS
                     QNHPEHHEISDVLVKIRSFIGKFKTVLSLNYDLIVYWASLLEDTTTGKLKDCFINRLF
                     NEQLDWSEFRDLHWKDREKGYKTTSLVFYPHGSLILAQDFSGQESKLHTNPRGEGNYL
                     QQISQQWSSATVTPVFVSEGTSKQKLSAIARSRYLDTVYREVLPEKKESIVIYGWSMG
                     ENDSHILDALKKSRPSRVAVSVYGTDTSGCNAIEGKLKAVFGEALLVEFFDSESANCW
                     CH"
BASE COUNT         4371 a         3661 c         3374 g         4476 t
ORIGIN      
        1 cctttcttta tgaatcaaca ttgccggtat taatattatg tttatcactc attcattaga
       61 ttcagcatat agagggatga caccagagac agggaaaagt aatccagatc ggcatagttc
      121 agtgaaaagt tactacagca cttatggata tgtaaccaac cacaaggaac tgaagcatga
      181 aactgaatga acgatatggc aagcctaatg cacactatat gaaacgtctg gaagagatta
      241 cccgttatgc gatggatgat tacgccagaa tactggcttt cagagtggat ttattactgc
      301 cagtggataa tagccataaa gggtatgact cagcggttat tacccgtttt atagcctcac
      361 tgaaagcgca gatagccgca tatttacaac ggcgcaggag agacggtaaa cgtgtttatc
      421 cctgtaagct gcgatatgca tgggtaagag aatttggtga actgaatggc aacaagcatt
      481 atcacatgct gttgttggtt aatcgggaag tattcagaaa caccatgctg aatacggaga
      541 attcagcgca acctcgcgga ttgctggtgg gaatggtggc gcgggcatgg gctgtggcgc
      601 ttaggcgtac cggagatatg gcaaataaag tcccctttca tttaccaggc gtctcttatg
      661 aacttgatcg cagaaatggc acaaatacgg actttttccg cagctacata taccacatca
      721 gttatctggc gaaggagtac accaaaaacc acgatgacgg tgaacgtaac tttggttgta
      781 gtcagggata agtaatttat taccggagaa gtgattgtaa tggcggagta tgtaacatca
      841 tcttaaatca aagggttagt gttgctgctc ctgtctttta tcaacgtaaa gacagaagga
      901 caatcgtcat gaacaatcaa ccgaataccg atactgacct gatgaatgac aaactggtga
      961 cgatggcatt tattaccacg tttactggcc tgaccgacaa atggttttat aaactgatta
     1021 gtgaaggcaa gtttccaaag cctatcaaac tggggcgtag ttcccgctgg cgggaaagtg
     1081 aagttaaacg ctggctgacg gaacgtattg cagaatcccg ctattaatcc ccctgacact
     1141 atcccaacac actttacccg taaacattaa ttcagcacaa agctatatca gtaaacgcct
     1201 gacagggtcg ttgctgtgca tctatcgacg cgtagcttca ttaaacccgg atgaaatacg
     1261 tttcgtttaa cattgtcggg aaataattaa ccggggaata gcctgcttat ttagcaggaa
     1321 ttccccggat gctgctacag gtaaaattag gaattaaatg tccagctacc cgtatcggtc
     1381 acaatgtcag cttcgatgat acggtcacac ttcatatatt tgaacgtagc cacatcaccg
     1441 aatttatatt tggctggcag ggttctgtta ccgacgctct ccattttaca gttccctctg
     1501 tttacccgaa tggatttcag caccacgttg tcatcagtga tagtgagctg aataacacca
     1561 tatctggtat tccagccttc attgctgacg gagctgccga actgaacttt aaacggtggt
     1621 gtcgtagatt gctgtgcctg ctgttgctga gagggatcag gtttattagc ctgctggcta
     1681 ccaacgccat taatgccatt ctgaaggagg ctcctggaaa ccttaatggt acccggtttg
     1741 catgatactg tgctggtttc ctgccccatt gaattcatgg cggtgtaata agcatcacct
     1801 gtaccatcat attctgagta attgactgca accgcgcctt cgatatcttc attttctcca
     1861 tgtaatgtga aagaagcggt tcctgctccc ggattagccg ccagagtctg tttcgcttcg
     1921 ccggaaggaa tatccagttt aataacagag ccgctctctt cggagtcacc acctggcgtt
     1981 tcaaaactga tataaaccgt attgcttacg tggctgagtg tcatttgctc gccgttatca
     2041 agcatacatt gcaggtcata ggtcccgact gcataagcag ggtggaatgc aaataaagat
     2101 acaaacaatg caggtataat gattagtttc attaagtact tccctatcca aaaaaatggt
     2161 cccaggcatt accagcccac tcgataagtt tgtctttccc ctttcgggta agctctttga
     2221 caggctttgg taacggaaaa gaatcaataa cgtcgtcgag tatcccggag attacgctgc
     2281 cgaagccgtt ttttgcttcc gtcttgacct cttcggtttt gaactcatcc ttaaactgcg
     2341 aagccgtgct acttttagcc cgatcaggca aagcccgaat aatagtctct accagacgaa
     2401 gaatgttgta gccctcatcg gcggagacag gtgtcaccgg atagtgcgta aaaccaaatc
     2461 gttcagtaat gtaggcttct ttcttttcga tgttgatgcg ctgtgcagga gatggctgat
     2521 gcagatgagt atcccactcg cggaatggct caattttatc aacctggttg aggatgaata
     2581 aaacgcgctc acccccacca gcagggagca ggacattgtt gtagaaatgc tcatccgcag
     2641 aaaaggcccg atcgtcacct tttattaccc acaggatgag atccagggag ggaagcaaat
     2701 tgcggtataa atcttcatac tccttatccc gccgggcatt ctcaccgaca ccaggtatat
     2761 caatgatctt tagcgaacgt ttgccgaagc gtatacgcaa ttcctgaact tcgcgagtac
     2821 atgcctcaac gtcgctaact gcacaaacct cccctttaaa cagcgcatta catacgctgc
     2881 ttttccccgc gccagttttt cccatgacac caatcacggg ttcatagtcg atggcttcac
     2941 tgattttgtt caacagcttt tcccgcagtg gctgagggaa aatatcaaga atgtccctga
     3001 tgtcgggtat gtgcggtgtg ctatcacctt tttgctcgtt gctaccatta cgttctggaa
     3061 atatattgcc ccacattccc aaaatcccgc cttcccttct ggtgacgcca ctggcatcag
     3121 taatcatccg gccttcgccc tttaccgggt catccatacg aggtctcctt tttaatttga
     3181 aataaacgtt gtctgttact ctctgaaggt ctgaattacg tcacttatga aacgaaagtg
     3241 agggagggct gaaataaact cttcactggc agtcagagcg tcaagttcag gctttctgcg
     3301 aaaagaggta tccagaacgg ttacggcgtt aacgtcttcc atccgaaaca cctgtaaatt
     3361 tctggtgtgg gtagcaacaa gaaaccagtt tttggactga aatatcagac ggcggggagc
     3421 tactgcttcg tagcttttac cgttaactaa taatgtaacg gcgcggtagt cacggatagc
     3481 ttccgccagt tccagaaaac aggcagtctg cgtgacggga gaagataatg ccccatgccc
     3541 aataagacac ggcgatacgc cggaattgct ggcaatcaac cagatcaact tgcggttctg
     3601 agccggaatg attcctgaaa ttcctgaatt acgtataaac gcaaatgtat tgggtaagtg
     3661 ctcccggcct gtttggcgga taagtcggta acttccgctg cgatgctcaa gatccagatg
     3721 gatcaggcgt tgctgaaaat cccgctgtag ggtgcgttct gagacaccaa attcatcagc
     3781 taatacttta agctccagag attcaccagc cagtaagcgg ctgataatga ctgatagcct
     3841 gacagacagg cggtcatgat gatcatgctg tgttttcatg gtggggacag tcctgttctc
     3901 ccaaaatatc atggtattga tacgggagat tatgccgtac aggtacgaca cgttatgtcg
     3961 tgccaaaggg gagttgattg aaaaaatagt ggggtaactg cagttatcta atactctgaa
     4021 atataagtta attgattaac tctaaggcaa ggtcatcatc acagaaacga cacaacctgt
     4081 cgcgttgcgc attgtaagaa agagggtatc tgatgcttac gaaaatgatt tagttctgaa
     4141 aatagttgca aatgaatcaa tacattcttt ttttttacaa aaagttggaa cgcataagcc
     4201 aattgacaat tatcaatgac aaatccttct agcgcacttc gttgatttaa tatagattta
     4261 ggatatccaa tctattcaca cataacgatt tgctccgctg aactaaccga gcaacattgc
     4321 aaaaaaatat accacgccga caactaagaa aagtactcgc ccgtataaag tatcaagtaa
     4381 atcaatgcga tgcagaataa acaccggccc ttaatgtgat agaaatcaca ttccttcagc
     4441 cctgcattcc ggcataatca tatcgggagg ttaaactgct cacgccatat ccctttttag
     4501 ggggatatct atgaataccg tttcaatcca ctaataaata aggtaataaa tggacgcaga
     4561 gaaaattcag tcagatatag atgctctttt gacatacatg aacgatgaca tggagaatct
     4621 tggtgaaaca ataaaaaccg ttacatttgg attcgaaaca ggccagaacg accgcattgc
     4681 tatagacgag ttgcttcact ggcgggaaga gtatttgttc aaaatcatca atatttgcca
     4741 ttcccgctct ctattacgta aggactatgg agattttgag catgtatcct tgacagaaga
     4801 gggtcagagt cgtgctatat caatcaaaca cggaaaaaat cgctcttacg agttaggagc
     4861 cagtatgcaa atcgcaacgt taaatgttca tgggcctgca caggttggta acagtaatac
     4921 gcaaaacatt cacaatgtat ttaatcaatt acttcagcag atcgagaaat cttccgcttc
     4981 tgatgaagaa aaacaggaag ccaaaaaacg cctggcgaaa tttttggaac atcctttggt
     5041 tagttctatt gtcggcggtc ttgctggttc tcttcctggt ctaatgggat gatggaagat
     5101 cttaaaaaaa cgatccgact gcgctgtgca ttctgccgtt cggaacagtt cgccttacct
     5161 tacgagagtt actccccccc ccaagtcata gctttgtcgt ttgtgccaac tgcgggaggg
     5221 aaaacgacgt cacatctctg ctcatcgttg ctaaatcgaa agggatggaa atagctgaag
     5281 agtgtgctga tgaactagtt gaacagatga cgaaagatct gtttaaaact tttaaaaaca
     5341 gtaaattcat aaagataaag taactgttac aaaagttaca tggaggacat atgtcaagga
     5401 ctaaagtact ttttgcttat tgccatgagt tagatcggtg tttatcgatt gatgaagcaa
     5461 gaacagaatt cttttcaaaa aaaccgcaag acagacaacg attcactttc tcctgcagcg
     5521 atcgcaactg caatgtcacc attagtggtg ttaattacca tgtcaaagcg gaagatggtg
     5581 aaaaattcaa agttgcacat tttcgttctc cacatcccca taatctggga tgtgaatgga
     5641 tgcagttttc tgaggaagct aaccagcccc gtcaggctag tgaaacagag gctgactaca
     5701 cagaaagaaa ggcaaggcag aagctaaatg attttatcaa ttgcttcgat ccttgtactg
     5761 atgagacaga ttctgggcaa aaaaaaactg gacaacttac atcactttcg caggtaaacc
     5821 atctcgtcaa agacaatgat ttgtctaacg ataaatccca caagtggagc cgttatacac
     5881 gaactaacca gctacagcgc ctagtagaca cctggcagga agccaagaaa actcttacat
     5941 acgacgagtt cagaacatta aaactaagag tcacaaacta cgggaaggtt tatttccatg
     6001 aatacataac gcatattcag aaagggctaa aaaaccaata tgaaggcgtc attttcggcg
     6061 gtggttcttt ggttaaacgc tatggtcgtg gctttctcat tcactttttc gataaacata
     6121 acgataaacc aattcgtctg tatatcgata agaaccttat ggagaaaggg cgattcagtc
     6181 attatattga tgaaatatta tcaacagaga atgtgagata ttttcgtgtc tttttgttaa
     6241 acccagaagt ttccgaaaaa aaagaccgaa ctggcaagcc agttatcaac cttgagatat
     6301 caagcctgcg acaattggcg atttactacg aactcaactc ttctgaagat aatgattttg
     6361 tttgacagta gcatgctaaa tttataaaaa ggagaagtat ttatggcccc tggatatgta
     6421 tacatattaa tcaacccaag catgccaggt ttaattaaaa taggtcgaac gctacgcgac
     6481 tcacgcatgc gtgcgcgtga actcttctcc accggagtgc caacaccgtt tcaagttgct
     6541 tttgagctgt ttgctgaaca acatgaagct ctcgaggcta cggtacataa tgaattgaat
     6601 gattttcgag ttaatgtggc acgcgagttt ttccgctacc cactcgacaa ggccgttgca
     6661 ttactaattc agctgccccc cctcccaaac tccactgaac agtatgtggc cgaagatata
     6721 acacaacggc ttagagaaaa atatccttta tatatacgtc cagaaattgt agcggtaagg
     6781 attgttcaaa tgcccggagg tgtctggcta aagataacga ctgagaaaga aatcgctgga
     6841 tacctcgtag atcagataat ccaacgaaca gatctagcat tcattgttga cacagacgag
     6901 gcattctttc ggtcagaaga tgatgtccgt ttaaatgcga ataaactggt aaatgattac
     6961 gatatttact ctatcgtcat gactacggac ctctttcatg attacgcctg ccaacaaata
     7021 acacgcgcgc atcatgccca gagaaatttc gtctgatgcg acaggcgtaa cgatacccca
     7081 aattcattac tcacagtaat ccatgtccag tttgggcact ttaactaaac gagaggaaac
     7141 accaacctcg ttttcaatca tcaagtgaac taaacgttcg ccatcaacta gaaccattcc
     7201 ctcaacggaa tgagcaaaat cccgcgcctg agaagtaaag ccggaagtag taataaatac
     7261 tccacgttta gccttttgtc cggccagcgc accatagaac gcctgaagtt cagggcgacc
     7321 aactgtgttc tgccagcgtt tagcctgcac gtaaactttt tccagtccca atttatcaag
     7381 tgaaataatg ccatcgatac taccatctcc tgctccacct acgcgctgaa gatcatcccg
     7441 atgtccacca tatcccaaac gatgaagtac atccaaaacg ataacctcaa aacgcgctgg
     7501 agaaacctgc aacaagtttt ccagtaactc ttttaacgct tgctcaagac gatcgtcagg
     7561 gctgcttttt gccagctctt cctggttcgg tgctgttggt cttggatcaa gatcgacagc
     7621 atctggctgc tgttttaact tcacattcat gaaatccaag gccaagtggt tcacttcctc
     7681 ttcggtgagt gccagggaat ggacctgtac ccatttaacc ccttctggcg ttaagcacca
     7741 tttccctctg gataaacttt gcgataatcc agctctcttc agacgatcat gtgcccaaca
     7801 gcccgatttt tataaactaa ctgaccgctg gctatcactt ctgctcgctg attatcatca
     7861 agattaagag catctgccgc agcttcatgc gcatcgcgat caggtactcc atcaggttta
     7921 cctgccagaa aacggagaac tggttcaata aatttatcgt aggtaggaac ggccatataa
     7981 acatccctga taatgttttt attagatcgg ttgcgtcaaa cctatcaact aaagtctgtt
     8041 tagttactta gcaattgagt gtacttccaa ctggcgagga gatgaacaca tacctcaatg
     8101 attttactaa ccaagataaa gtctcatctc cacatcataa atcagaaaat atcaccccag
     8161 ccatctcatc caaccgagct ttgactactt cccactcggg cataacttgt cccatcagac
     8221 ggtaaaaacg ctcgctatgg ttgtgttcgg caatatgaca cagctcatgc agcatcacgt
     8281 aattaataca ctcatgggtg attaagtgtc agacttccac cgagttaaca tatgtctaat
     8341 tgttccgaaa tgcttgacaa gcatagctgt taactcattg atttttattt ataaaaaagt
     8401 aaattacagg gccacctatg agttggccct tgcatattaa aacccgcgca tcccattgat
     8461 aaacgtatca attacatccc gaacacgttc gtaaacccga gctcgaacgc ttttgcgttc
     8521 cagaatcttt ggtgtccaag tcagcgtttc cgccacctca tcctctctcg gcatcacgtc
     8581 ggtaaagagg tagttatcca gcaagccagc cagtttgatg gaatcgagtc gctcttcgct
     8641 gcacaaggtt gccagcgcct tatctttttc ctgttcgaca taggcttcaa acacctgagt
     8701 gatatcctcg tcatgcgagg catgcagtag gcttttttcg ataaagttat caataagctc
     8761 acgtttacta cgcagcgtga cgtcgctgtc gacaatatcc atgatctttt tcttctgctc
     8821 ctgctgctct tttagcggca tagcctgaat gcccgccagc agacgcagaa tgtagccgac
     8881 gttaatttcg tcgcgatgaa tcagcgagac ttcaaaatcc acatcgtcaa gaatcgagac
     8941 tttctctttg ctggagtgct ttttcacctt gtcatggata tcgaggtatt tactggtaaa
     9001 atctgccagc gtctggtctg aaagcggttt agccgcatcg tcaaaatcgg caaagctttc
     9061 gaggatgttt ttcaaacgca tcacatcgcg gaatgccgtt acaaacgcca gttgtgcctc
     9121 ttcatccggt aaatcgtcca cgctatccac cgttggcgtc agcgttaaca agcgatcgac
     9181 agcgtcctga taatccccga agtactcgtc aaagggtttg accagtacca cagaagaagc
     9241 atctttgttc gagaacaagg caatagcatc atcggtagct ttcttgagat tgcggaagca
     9301 gacgatattc ccgtggcttt tcttctcgtt gagaatgcgg ttggtgcggg aaaacgcctg
     9361 caccaggccg tgaaaacgga ggtttttatc gacgtacagc gtgttcagcg ccgggctgtc
     9421 gaagccggtg aggaacatat tcaccaccag cagaatatcg atgcctttct ctttggtgcg
     9481 ctgagcaata tttttgtagt aattgtagaa gctctttgaa tcattggccg agtagctggt
     9541 gccatacagc tcgttataat ccttgatgaa actatccaga tgatcgcgac tcgaggtatc
     9601 aactttcgag ccctgcggca gactaatatc ctcttcatcc agcagaccgt ttaccgcctc
     9661 tttatcactt ttgtccgctt cattggcggc ataagtgaag atggtggcaa tttttaatgg
     9721 cttgtagttg gcatccgccg cctgcagtgc ggcctgcttt tctttaaaga gtttgtagta
     9781 ggtgatcagc atatcgactg acccgacgca gaacatcgcg gtgaactcgc gattgcgggt
     9841 cttggcgtta tggtgggcaa ggatgtagtc ggtcactttg cccaaccgga tgtcgctttc
     9901 catcacctct ttggtgtcga tatcctccac atcgatatcg atctcattgc tgctttcttt
     9961 gcgcttgtaa gtaccgacat attccacggc aaagcgcaga acgttttcat cccggatggc
    10021 gtcgacaatc acgtatttat gcaggcattt atcgaacaac agttcggtag tctgtttgac
    10081 accattggtg atattgacgt tatcggcaaa gattggcgtc ccggtaaaac caaacagctg
    10141 tgcgttctcg aagaagttca cgatcgcttt atgcgtgtca ccgaactggc tgcggtggca
    10201 ttcatcgaag ataaagacga tgcgctcttt gcgcaggtgt gcgagctgag ctttataccc
    10261 ttcatgagtg atagcgttat tcagtttctg aatagtagtc agcgtcagtt tggtgctgct
    10321 ttgcttgaga tgttttatca gcgtactggt gttgttggtg gtatccacgc tgtctttcgc
    10381 aaaaccgttg aactctttcg ccgtctgata atccagatcc cggcgatcaa ccacaaaaat
    10441 gacccgatcc acttccggca tttccatgat cacctggctg gctttgaacg aggtcagcgt
    10501 tttaccggag ccagtggtgt gccagatata accgttctcc ggacgtccct gtttgtggag
    10561 attctccttc acatggcgga tcatcgcctc acaggcataa aactggtaag ggcgcatcac
    10621 catcaggcac ttacgggttt cattcagcac cgtgtagtgg gtgatcattt tggcgatgtg
    10681 acagactttc agaaatgcgt cggtgaattt ctccagcgct ttgagcgggt tattcttttc
    10741 atcggtccag aagaaggtct gctcgaagtg gttggcgcgg ttgttggcgt aatatttggt
    10801 gtccacgccg ttgctgataa taaagatctg cacatactgg aacaggccgc tgcctaccca
    10861 gaaggcatcc cgatggtaac gatcgatctg gttaaaggcc tctttcagcc ccaggccgcg
    10921 acgcttaagt tcgatttgca ccagcggcag gccgttgatc agtagcgtga cgtcgaagcg
    10981 ggttttacgc gtgttttgct cggcgttgaa ctgcttcacc tggttcgtca cctgaaactc
    11041 gttctggcac cactcctctt tattgataaa gctgagccac ttctcggtgc cgtcatcgcg
    11101 caacaattgg tagcgatcgc gaaggatttt agcgcgatcg ataacttcat tacctttggt
    11161 caggtgcaaa aaaatacggt taaattcacc gtcagttagc ggagaagatt ccagccggtt
    11221 atggatttca atctgctttc gcaggttggc tttcatactg gtttcatcgg tgacattcac
    11281 ccgctcgtag tgcatgccaa ccagttgttc caccagctgc gtttctaact cgtattcgct
    11341 ttgcgttgcc atcagtatgt agtgcctttc gttattgtgt tggcccggag tcggtcgggc
    11401 cggataattt ttatacaaac atttgctgga gcaggccctg cttccaggtt tttaacttgt
    11461 ccagttctgc ttttttagcg gcgattttat cgtcaagggt ggagagaaag ctggaaattt
    11521 tgttttgctc agataaacaa ggaatattaa cctcaatctt ttctatagta tttttagaaa
    11581 ggcttggtac tccggatgcc tcgttataaa ctttccagtt tattgttaaa aacaattgat
    11641 acacaaattt tgggtgcgcg cctttaaatt catgagtata aaataaagta tctacagtcc
    11701 aaattttccc attgtagtaa aatggcttat caatagtccc ttttcggcca atgcataccg
    11761 tttccccttc aaaaagatac tgatcaacca ttgtcataaa accaccagta ccaaaaacag
    11821 ggacattacc agcattcaaa tgtttataat ctcgaccgct accaatcgtt agaatattgc
    11881 ctatgaattt tctattccac tcagaaaact cctccccatt ctcatcctta aaaagtaatt
    11941 cttgactaaa aatcttctgc atcatgcctt ttttgtactg ggacagcaag ttgtactgct
    12001 tgttcagtaa cgcgattttt tcgtcaacag agaataggaa gtcggcgatc ttagtttgtt
    12061 catcaataca aggaactgga attattgcat tattgaaatc ttttaaatta taattgtttc
    12121 taccatcacc tgtcgccatg cttaaagcat atttaatata atagggtgaa ttaaaatact
    12181 gacatagata ttttgagtta atgatggttg gattgggtcg agtattcaag gttgcaaatc
    12241 cgaggcatcc gtcaagctcc tcatcaatta ctgcagatac accaatatcg ccagtgtgga
    12301 ctgtaacaac atcacctgac tttaattttc tactcccgta ttgttcggca aaacttatat
    12361 ctaacttgat aagttttgat ttttcgacat aattttcttt tatatcggag ttacgaatca
    12421 atttgacacc attatctaca tagctagatg tcatagtagt tactaaaccg ataaacgttt
    12481 ttgatatatc agtgatcttt ttcgactgcc atttatattc atcattattt aaaaatcgat
    12541 agaatctcag cttaggcacc atcacttatt ttcctccgcc agcgcaaacg gcgcatcaat
    12601 tcccagctcc ttacagaacc cagcaatggt cttatcaatc tccgccactt tcccttccag
    12661 atcgcgaatt tcctgcgcca cagcgttgag atcaatttct tcttcagctt caaaagtatc
    12721 gacatagcgc gggatattga ggttgtaatc gttgttacgg atctcttcca gcgttgcgat
    12781 atgactgtat ttctcaatcg ctttccagtc gttataagta tcggcaatct tatcgatatg
    12841 ttccggcagc aggcggttct gggttttcac cttctcaaag tcattgctgg cgtcgataaa
    12901 cagaatgctg tcgttatgct tgcggcattt acgtagcaca agaacgcagg tcggaatgct
    12961 ggtgccgtag aagatattgg caggcaggcc gatcaccgca tcaatgcagt tcagcttctc
    13021 aatcataaac tgacgaatat ggccttccgc tgcgccacgg aataaaaccc catgaggcag
    13081 aaccactgcc atagtgccgt catcttccag gtgatgaaac atatgctgta aaaacgccat
    13141 gtctgctttg ctggacggag ccagtttgcc gtactgggca aagcggtcat cgttcataaa
    13201 cagcgggctg gcgctccact tggcggagaa cggtgggtta gcaacgatgg cgtcaaattt
    13261 cagatgcgta tgctgcggat gctccagcgt atcttcctga atgatctcaa aatccgcata
    13321 gtgaaccccg tgtaggatca tgttcatacg cgccaggtta taggtagtac ggttcatctc
    13381 ctggccgtag attttaccca cagatgaggc ttcacgtttc acgcgcagta acaaagagcc
    13441 agagccacag gtcgggtcat acacgttttt cagcttcagc ttatgagtgg tgacgatttt
    13501 agccagcagg gtggaaaccg gctgcggggt atagaactcc ccggctttct tacctgcgcc
    13561 tgaagcgaat tggccgatca ggtactcata ggcatccccc agaatgtcgg aactcgcttc
    13621 gctcaggtta aagcttagtt tgtcgagttc agtgacgact ttaccgatca gctcattttt
    13681 agctttcgca gtgttgccaa gcttgcttga acctaaatca agatcttcaa acaggttgct
    13741 gaagtcatct gcagagtcgg tacccagcgt gctctgttca atgctgcgta gggtagtggc
    13801 gaggtcttcc agaataaagc cagtacctgt gcctttgggg tctttagcca cacgttctgc
    13861 catagtgtgg aacaaatccg ttggcggcaa gaaatagcct aaggtctgga tactgtcctc
    13921 ttttaccgca tcaacgatat cctgatacgc tggatgttcc acagtcagtt cattgtactg
    13981 aatgtcatct tccgccagaa ttttgttcgc atacgcgaca aatttttctg acaggtattt
    14041 gtagaagata aaacctaggc agtagtcgcg gaattcatcg gcattcattt taccgcgcag
    14101 ggtatcggca atattccaca ggcggccttg cagttcactt aaattttgcg cttcaatctg
    14161 aggagacata ataaacctta atcattgatt attttgataa attttctatt ttggcaaaat
    14221 aatattgttg ctttaatttc atcaagttgt actcgtagta atctttcatc ctgagatgaa
    14281 gatacagctc acctatcgct tgttgctttt ttagtccagg taaatcgagt tcaaattgac
    14341 gaaccatttg cagagttaac ttaggtacag ttgacccctg tagagcagat gatttttgtt
    14401 gggctacagc tttgctctca ttgaaatgcc aaatccaaaa taatggagag actctgctta
    14461 catccaactc aaccttaaca aagttattgc ccaatattcg ccctgcatga tcctgcttaa
    14521 caagcacagc tttagcattc gctaagttaa taatgacatc attttctctt gtcactttta
    14581 gtaacgatgt actcttaacc acctgttctt tatcagtaac tatcacttct ggttgccagt
    14641 catcgatctg atgcaactgc gctgtataaa agcgatatac tttacctgac tcttccggct
    14701 ctatgcgtga caggtttact ccggggataa aatttgcaat gtcgctatag actatttttc
    14761 ccatttttat cctcgtataa tagggggcga cgcccccttc tttcgcttat ttaatggcac
    14821 cagcagttag cgctttcact gtcgaaaaac tcgacaagca aagcttcacc aaacaccgct
    14881 tttagtttcc cttcaattgc attacagcca gaggtatcag tgccatacac tgaaaccgca
    14941 acccttgatg gacgactttt tttcagtgca tcgagtatat ggctgtcatt ttcccccata
    15001 ctccatccat agatcacgat actttccttt ttctctggta gtacttcacg gtagacagtg
    15061 tcaagataac gactacgagc tatagcactt agtttttgct tacttgtccc ttcagaaaca
    15121 aaaactggtg taactgttgc agaactccat tgctgagaaa tttgctggag ataatttcct
    15181 tcaccacgag gattagtatg taatttggac tcttgaccac tgaaatcttg ggccaaaatc
    15241 aaagaaccat gtgggtagaa gactaagctt gtcgtcttgt aacctttttc acgatccttc
    15301 caatgtaagt ctctaaactc actccaatct aactgttcat taaacaaacg gttgatgaag
    15361 caatccttta attttccggt agttgtatcc tcaagcaaag atgcccagta cacaataaga
    15421 tcataattca gagataaaac cgtcttgaat ttgcctataa aagaacgaat tttaactaac
    15481 acatccgata tctcatgatg ctcaggatga ttttggctca cacattgaat caacagattt
    15541 ctaagtaact tatacccctc agtcgttgca ttttcaggca caccgagctg ttgatttact
    15601 tgagttgcaa tccatagacg atgcagaatc aactcaaaat cttgcgtatc aaatagttcg
    15661 aacaaggctg tcagctgccc atcagaactg agctcggtca acttctgaca taagctacga
    15721 taggcaaaat cgctattaac agcgatactt gcaccattcc ccaaaagtaa agtatcatta
    15781 taagcggcct caatctcggc ccattgttta attgttacca tgcttcaacc ctcattttag
    15841 atttgtgggt attccgccaa gaactcaccc actaatgttt aa
//
LOCUS       DADSYM010000114        15870 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2415
            SAMN08039579-rid11723173.denovo.115, whole genome shotgun sequence.
ACCESSION   DADSYM010000114 DADSYM010000000
VERSION     DADSYM010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039579
            Sequence Read Archive: SRR6362533
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15870)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15870)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980162.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 03:39:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,484
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 4,347
            CDSs (with protein)               :: 4,347
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 43 of 85
            Pseudo Genes (incomplete)         :: 37 of 85
            Pseudo Genes (internal stop)      :: 19 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15870
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039579-rid11723173.denovo.115"
                     /strain="LGE2415"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8302 N 95.9628 W"
                     /collection_date="2015-08-24"
     gene            complement(227..1555)
                     /gene="dsdA"
                     /locus_tag="J1Y83_003187"
     CDS             complement(227..1555)
                     /gene="dsdA"
                     /locus_tag="J1Y83_003187"
                     /EC_number="4.3.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine ammonia-lyase"
                     /protein_id="HBA7631310.1"
                     /translation="MENAKMNSLIAQYPLVKDLVALQETTWFNPGTTSLAEGLPYVGL
                     TEQDVQDAHARLSRFAPYLAKAFPETAATGGIIESELVAIPAMQKRLEKEYQQPISGQ
                     LLLKKDSHLPISGSIKARGGIYEVLAHAEKLALEAGLLTLEDDYSKLLSPEFKQFFSQ
                     YSIAVGSTGNLGLSIGIMSARIGFKVTVHMSADARAWKKAKLRSHGVTVVEYEQDYGV
                     AVEEGRKAAQSDPNCFFIDDENSRTLFLGYSVAGQRLKAQFAQQGRIVDADNPLFVYL
                     PCGVGGGPGGVAFGLKLAFGDHVHCFFAEPTHSPCMLLGVHTGLHDQISVQDIGIDNL
                     TAADGLAVGRASGFVGRAMERLLDGFYTLSDQTMYDMLSWLAQEEGIRLEPSALAGMA
                     GPQRVCASVSYQQMHGFSAEQLRNATHLVWATGGGMVPEEEMNQYLAKGR"
     gene            complement(1573..1797)
                     /locus_tag="J1Y83_003188"
                     /pseudo
     CDS             complement(1573..1797)
                     /locus_tag="J1Y83_003188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416866.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine transporter DsdX"
     gene            1958..3016
                     /locus_tag="J1Y83_003189"
     CDS             1958..3016
                     /locus_tag="J1Y83_003189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBA7631311.1"
                     /translation="MDLASDVCFCYSARFTANGLGMASLKDVARLAGVSMMTVSRVMH
                     NAESVRPATRDRVLQAIQTLNYVPDLSARKMRAQGRKPSTLAVLAQDTATTPFSVDIL
                     LAIEQTASEFGWNSFLINIFSEDDAARAARQLLAHRPDGIIYTTMGLRHITLPESLYG
                     ENIVLANCVADDPALPSYIPDDYTAQYESTLHLLAAGYRQPLCFWLPESALATGYRRQ
                     GFEQAWRDAGRDLAEVKQFHMATGDDHYTDLESLLNAHFQSGKPDFDVLICGNDRAAF
                     VAYQVLLAKGVRIPQDVAVMGFDNLVGVGHLFLPPLTTIQLPHDIIGREAALHIIEGR
                     EGGRETRIPCPLLIRCST"
     gene            complement(3024..4457)
                     /locus_tag="J1Y83_003190"
     CDS             complement(3024..4457)
                     /locus_tag="J1Y83_003190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 32 protein"
                     /protein_id="HBA7631312.1"
                     /translation="MTQSRLHAAQNALAKLHERRGNTFYPHFHLAPPAGWMNDPNGLI
                     WFNDRYHAFYQHHPMSEHWGPMHWGHATSDDMIHWQHEPIALAPGDENDKDGCFSGSA
                     VDDNGVLSLIYTGHVWLDGAGNDDAIREVQCLATSLDGIHFEKQGVILTPPEGIMHFR
                     DPKVWREADTWWMVVGAKDPGNTGQILLYRGSSLREWTFDRVLAHADAGESYMWECPD
                     FFSLGDQHYLMFSPQGMNAEGYSYRNRFQSGVIPGMWSPGRLFAQSGHFTELDNGHDF
                     YAPQSFLAKDGRRIVIGWMDMWESPMPSKREGWAGCMTLARELSESNGKLLQRPVHEA
                     ESLRQQHQSISPRTISNKYVLQKNAQAVEIQLQWALKNSDAEHYGLQLGTGMRLYIDN
                     QSERLVLWRYYPHEHLDGYRSIPLPQGDMLALRIFIDTSSVEVFINDGEAVMSSRIYP
                     QPEKRELSLYASHGVAVLQHGALWQLG"
     gene            4673..5596
                     /locus_tag="J1Y83_003191"
     CDS             4673..5596
                     /locus_tag="J1Y83_003191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001274886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoimidazole riboside kinase"
                     /protein_id="HBA7631313.1"
                     /translation="MSAKVWVLGDAVVDLLPESDGRLLPCPGGAPANVAVGVARLGGT
                     SGFIGRVGDDPFGALMQRTLLTEGVDITYLKQDEWHRTSTVLVDLNDQGERSFTFMVR
                     PSADLFLETTDLPCWRHGEWLHLCSIALSAEPSRTSAFTAMTAIRHAGGFVSFDPNIR
                     EDLWQDEHLLRLCLRQALQLADVVKLSEEEWRLISGKTQNDRDICALAKEYEIAMLLV
                     TKGAEGVVVCYRGQVHHFAGMSVNCVDSTGAGDAFVAGLLTGLSSTGLSTDEREMRRI
                     IDLAQRCGALAVTAKGAMTALPCRQELESEK"
     gene            5658..6905
                     /locus_tag="J1Y83_003192"
     CDS             5658..6905
                     /locus_tag="J1Y83_003192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBA7631314.1"
                     /translation="MALNIPFRNAYYRFASSYSFLFFISWSLWWSLYAIWLKGHLGLT
                     GTELGTLYSVNQFTSILFMMFYGIVQDKLGLKKPLIWCMSFILVLTGPFMIYVYEPLL
                     QSNFSVGLILGALFFGLGYLAGCGLLDSFTEKMARNFHFEYGTARAWGSFGYAIGAFF
                     AGIFFSISPHINFWLVSLFGAVFMMINMRFKDKDHQCIAADAGGVKKEDFIAVFKDRN
                     FWVFVIFIVGTWSFYNIFDQQLFPVFYAGLFESHDVGTRLYGYLNSFQVVLEALCMAI
                     IPFFVNRVGPKNALLIGVVIMALRILSCALFVNPWIISLVKLLHAIEVPLCVISVFKY
                     SVANFDKRLSSTIFLIGFQIASSLGIVLLSTPTGILFDHAGYQTVFFAISGIVCLMLL
                     FGIFFLSKKREQIVMETPVPSAI"
     gene            complement(6985..7137)
                     /locus_tag="J1Y83_003193"
     CDS             complement(6985..7137)
                     /locus_tag="J1Y83_003193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001316510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA7631315.1"
                     /translation="MHSLMNSQARDTRDRSLNDDNVLEISEPRIRSTFERLYQNMNEA
                     FGLGDY"
     gene            complement(7347..7421)
                     /locus_tag="J1Y83_003194"
     tRNA            complement(7347..7421)
                     /locus_tag="J1Y83_003194"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7386..7388),aa:Arg,seq:cct)
     gene            complement(7497..8429)
                     /locus_tag="J1Y83_003195"
     CDS             complement(7497..8429)
                     /locus_tag="J1Y83_003195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HBA7631316.1"
                     /translation="MDNDKIDQHSDEIEVESEEKERGKKIEIDEDRLPSRAMAIHEHI
                     RQDGEKELERDAMALLWSAIAAGLSMGASLLAKGIFHVELEGVPGSFLLENLGYTFGF
                     IIVIMARQQLFTENTVTAVLPVMQKPTMSNVGLLMRLWGIVLLGNILGTGIAAWAFEY
                     MPIFNEETRDAFVKIGMDVMKNTPSEMFANAIISGWLIATMVWMFPAAGAAKIVVIIL
                     MTWLIALGDTTHIVVGSVEILYLVFNGTLHWSDFIWPFALPTLAGNICGGTFIFALMS
                     HAQIRNDMSNKRKVEARQKAERAENIKKNDKNPA"
     gene            8723..9478
                     /gene="mlaA"
                     /locus_tag="J1Y83_003196"
     CDS             8723..9478
                     /gene="mlaA"
                     /locus_tag="J1Y83_003196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid-binding lipoprotein MlaA"
                     /protein_id="HBA7631317.1"
                     /translation="MKLRLSALALGTTLLVGCASSGTDQQGRSDPLEGFNRTMYNFNF
                     NVLDPYIVRPVAVAWRDYVPQPARNGLSNFTGNLEEPAVMVNYFLQGDPYQGMVHFTR
                     FFLNTILGMGGFIDVAGMANPKLQRTEPHRFGSTLGHYGVGYGPYVQLPFYGSFTLRD
                     DGGDMADSLYPVLSWLTWPMSVGKWTLEGIETRAQLLDSDGLLRQSSDPYIMVREAYF
                     QRHDFIANGGELKPQENPNAQAIQDDLKDIDSE"
     gene            complement(9540..10880)
                     /gene="fadL"
                     /locus_tag="J1Y83_003197"
     CDS             complement(9540..10880)
                     /gene="fadL"
                     /locus_tag="J1Y83_003197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416846.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid transporter FadL"
                     /protein_id="HBA7631318.1"
                     /translation="MSQKTLFTKSALAVAVALISTQAWSAGFQLNEFSSSGLGRAYSG
                     EGAIADDAGNVSRNPALITMFDRPTFSAGAVYIDPDVNITGHSDLTGQSANAKNIAPT
                     AWVPNLHFVAPINDQFGWGASITSNYGLATEFTDNYAAGIYGGKTDLQTINLNLSGAY
                     RLNNSWSFGLGFDAVYAKAKIERYAGSLGPLLSQQLIAQGVPASLTNGINTPDSQIAH
                     LKGDEWGFGWNAGILYELDKDNRWGLTYRSEVKIDFDGDYKSSINPNLNNILGNMGLP
                     YGTMGATQNGSLTLNLPEMWEISGYNRVAPQWAIHYSMAYTSWSQFQELKAKGDSGQT
                     LFYKDEGFRDAYRIALGTTYYYDKNWTFRTGIAYDDSPVPADKRSISIPDQDRLWLSA
                     GLTYAFNEDASIDVGASYMHGQKVKFTEGEGAAAYSFESEGKAWLFGTNFNYAF"
     gene            11252..11536
                     /locus_tag="J1Y83_003198"
     CDS             11252..11536
                     /locus_tag="J1Y83_003198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF406 family protein"
                     /protein_id="HBA7631319.1"
                     /translation="MSKCSADETPVCCCMDVGTIMDNSDCTASYSRVFANRAEAEQTL
                     AALTEKARSVESEPCKITPTFTEESDGVRLDIDFTFACEAEMLIFQLGLR"
     gene            11716..13026
                     /gene="fadI"
                     /locus_tag="J1Y83_003199"
     CDS             11716..13026
                     /gene="fadI"
                     /locus_tag="J1Y83_003199"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase FadI"
                     /protein_id="HBA7631320.1"
                     /translation="MGQVLPLVTRQGDRIAIVSGLRTPFARQATAFHGIPAVDLGKMV
                     VGELLARTEIPAEVIEQLVFGQVVQMPEAPNIAREIVLGTGMNVHTDAYSVSRACATS
                     FQAVANVAESLMAGTIRAGIAGGADSSSVLPIGVSKKLARVLVDVNKARTMSQRLKLF
                     SRLRLRDLMPVPPAVAEYSTGLRMGDTAEQMAKTYGITREQQDALAHRSHQRAAQAWS
                     EGKLKEEVMTAFIPPYKQPLVEDNNIRGNSSLADYAKLRPAFDRKHGTVTAANSTPLT
                     DGAAAVILMTESRAKELGLVPLGYLRSYAFTAIDVWQDMLLGPAWSTPLALERAGLTM
                     SDLTLIDMHEAFAAQTLVNIQLLGSERFARDVLGRAHATGEVDESKFNVLGGSIAYGH
                     PFAATGARMITQTLHELRRRGGGFGLVTACAAGGLGAAMILEAE"
     gene            13026..15170
                     /gene="fadJ"
                     /locus_tag="J1Y83_003200"
     CDS             13026..15170
                     /gene="fadJ"
                     /locus_tag="J1Y83_003200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid oxidation complex subunit alpha FadJ"
                     /protein_id="HBA7631321.1"
                     /translation="MEMASAFTLNVRLDNIAIITIDVPGEKMNTLKAEFASQVRAIIK
                     QLRENKELRGVVFVSAKPDNFIAGADINMIGNCKTAQEAEVLARQGQQLMAEIHALPI
                     PVIAAIHGACLGGGLELALACHGRVCTDDPKTVLGLPEVQLGLLPGSGGTQRLPRLIG
                     VSTALEMILTGKQLRAKQAVKLGLVDDVVPHSILLEAAVELAKQDRPSSRPLPVRERI
                     LAGPLGRALLFKMVGKKTEHKTQGNYPATERILEVVETGLAQGTSSGYDAEARAFGEL
                     AMTPQSQALRSIFFASTDVKKDPGSDAPPAPLNSVGILGGGLMGGGIAYVTACKAGLP
                     VRIKDINPQGINHALKYSWDQLEGKVRRRHLKASERDKQLALISGTTDYCGFAHRDLI
                     IEAVFENLELKQQMVAEVEQNCATHTIFASNTSSLPIGDIAAHAVRPEQVIGLHFFSP
                     VEKMPLVEIIPHASTSAQTIATTVKLAKKQGKTPIVVRDKAGFYVNRILAPYINEAIR
                     MLTEGERIEHIDAALVKFGFPVGPIQLLDEVGIDTGTKIMPVLEAAYGERFSAPANVV
                     SSILNDDRKGRKNGRGFYLYGQKGRKSKKQVDPAIYPLIGAQGQGRLSAPQVAERCVM
                     LMLNEAVRCLDEQVIRSVRDGDIGSVFGIGFPPFLGGPFRYIDSLGAGEVVAIMQRLA
                     TQYGSRFTPCDRLVEMSERGESFWKTTATDLQ"
     gene            15373..15858
                     /gene="sixA"
                     /locus_tag="J1Y83_003201"
     CDS             15373..15858
                     /gene="sixA"
                     /locus_tag="J1Y83_003201"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohistidine phosphatase SixA"
                     /protein_id="HBA7631322.1"
                     /translation="MQVFIMRHGDAALDAASDSVRPLTTNGCDESRLMANWLKGQKVE
                     IERVLVSPFLRAEQTLEEVGDCLNLPSSAEVLPELTPCGDVGLVGAYLQALTNEGVAS
                     VLVISHLPLVGYLVAELCPGETPPMFTTSAIASVTLDESGKGQFNWQMSPCNLKMAKA
                     I"
BASE COUNT         3835 a         3783 c         4067 g         4185 t
ORIGIN      
        1 agcagtcact ttctatttct gcaaatgaga ttttccgtat ggcggctgtt gcttttatct
       61 tacttacggt tttggtttgg tttgcgaaac cgccgtttac agcgaaaggc gttgggtgat
      121 ggaaaaagag gaggctatag caggtaaaac ggcagaatct taagtctgcg tcatccgcag
      181 catcgctcac ccagggaaag gatggcgatg ctgcgttgaa acgttattaa cggccttttg
      241 ccagatattg attcatctct tcttccggca ccattccacc tcccgtcgcc cacaccagat
      301 gagtggcatt acgcagttgt tctgcgctga aaccgtgcat ctgttggtaa cttactgatg
      361 cgcacacgcg ctgaggcccg gccatacccg ccagtgccga tggttcaaga cgaatacctt
      421 cttcctgcgc cagccagcta agcatgtcat acatggtttg atcgctaagg gtatagaagc
      481 catccagtag acgctccatt gcccgcccga caaagcctga tgcgcgacca actgcaaggc
      541 catccgctgc ggtaaggttg tcgatgccaa tatcctgaac agaaatctga tcgtgtaatc
      601 cggtatggac gcctaataac atacacgggg agtgtgttgg ttcggcaaaa aagcagtgaa
      661 catgatcgcc aaacgccagt ttaagtccga atgcgacgcc accaggacca ccgccaacac
      721 cacacggcag atagacaaac agagggttat cagcatcgac gatgcgacct tgctgagcaa
      781 attgcgcctt aagacgctgg cctgcgacgg aatatccaag gaacaacgtg cgggaatttt
      841 cgtcatcaat aaagaaacag ttcgggtcag actgcgcggc tttacgtcct tcctcgacgg
      901 caacaccata atcttgctcg tattccacga cagtaacgcc atgactgcgc agtttcgctt
      961 ttttccaggc gcgggcatca gcagacatat gcaccgttac tttaaagccg atgcgggcgc
     1021 tcataatgcc gattgataac cccagatttc cggttgatcc cacagcaata ctgtattggc
     1081 taaagaactg tttaaactcc ggggaaagca gtttgctgta gtcatcttca agcgtcagca
     1141 accccgcttc cagagccagt ttttctgcat gtgccaggac ttcataaatc ccgccgcgtg
     1201 cttttatgga gccggaaata ggcaaatggc tatctttttt cagtaacagt tgcccactga
     1261 tcggttgttg atattccttt tccagccgtt tttgcatagc cggaatggca accagttctg
     1321 attcaataat ccccccagtg gcagccgttt caggaaatgc ttttgccaga tagggtgcaa
     1381 aacgagataa gcgcgcatgg gcgtcctgaa catcctgttc ggtcaggcca acataaggta
     1441 aaccttcagc caatgaggtc gtgccaggat taaaccaggt ggtttcttga agagcaacca
     1501 gatccttgac caacggatac tgggcgatga gcgagttcat tttagcgttt tccatagtac
     1561 gtctctttgc gcttagatga taaatgacag caggaatgtg cccgccagag cgatgactga
     1621 agcgataaat gtcgctgtcg tatagtattt aaatgtttca ttgagcgtag cgccgcaata
     1681 ttgcttcacc agccagaaaa gcgagtccgt aacgatcgtg cagccaatcg caccggaacc
     1741 gatagcaatc gcaataattt ccgggctgat gtcgggatac agcggcagca caatgatcca
     1801 tatttatatc ttcttctcca ggttggcatg ttttacctac ttgcctacat ggtgactcgt
     1861 tggcgagaac agaggcgaaa ttgggtacaa ttgtccggga attattgtta ctttttattg
     1921 tattgccgtg tcgggtgata gggcgtcgag aatatcgttg gatctcgcca gtgacgtctg
     1981 tttctgctac agtgcccgtt ttacggcaaa cggcttgggt atggcttcat taaaggatgt
     2041 cgcacgcctg gcgggagtgt cgatgatgac agtctcccgg gtgatgcata atgcagaatc
     2101 tgtgcgtcct gcaacgcgtg accgcgtatt gcaggcaatc cagaccctga attatgttcc
     2161 tgatctttcc gcccgtaaaa tgcgcgctca aggacgtaag ccgtcgactc tcgccgtgct
     2221 ggcacaggac actgctacca ctcctttctc tgttgatatt ctgcttgcca ttgagcaaac
     2281 cgccagcgag ttcggctgga atagtttttt aatcaatatt ttttctgaag atgacgctgc
     2341 ccgcgcggca cgtcagctgc ttgcccaccg tccggatggc attatctata ctacaatggg
     2401 gctgcgacat atcacgctgc ctgagtctct gtatggtgaa aatattgtat tggcgaactg
     2461 tgtggcggat gacccagcgt tacccagtta tatccctgat gattacactg cacaatatga
     2521 atcaacactg catttgctcg cggcgggcta tcgtcaaccg ttatgcttct ggctaccgga
     2581 aagtgcgttg gcaacagggt atcgtcggca gggatttgag caggcctggc gtgatgctgg
     2641 acgagatctg gctgaggtga aacaatttca catggcaaca ggtgatgatc actacaccga
     2701 tctcgaaagt ttactcaatg cccacttcca atctggcaaa ccagattttg atgttctgat
     2761 atgtggtaac gatcgcgcag cctttgtggc ttatcaggtt cttctggcga agggggtacg
     2821 tatcccgcag gatgtcgccg taatgggctt tgataatctg gttggcgtcg ggcatctgtt
     2881 tttaccgccg ctgaccacaa ttcagcttcc acatgacatt atcgggcggg aagctgcatt
     2941 gcatattatt gaaggtcgtg aagggggaag agagacgcgg atcccttgcc cgctgttgat
     3001 ccgttgttcc acctgatatt atgttaaccc agttgccaga gtgctccatg ttgcagcaca
     3061 gccactccgt gggaggcata gagcgacagt tcccgttttt ctggctgcgg atatattcgg
     3121 ctactcatta ccgcctcccc gtcgttaata aatacttcca cggatgatgt atcgataaat
     3181 atccttaggg cgagcatgtc accctgcggg aggggaatac tacggtagcc gtctaaatgc
     3241 tcgtgtgggt aataccgcca caaaacaagc cgctcagatt ggttatcaat atacagccgc
     3301 attcctgtgc cgagttgtaa tccgtaatgt tcggcatcac tgttcttcag cgcccactgc
     3361 aactgaatct caactgcttg cgcgtttttc tgcaaaacat atttattgct gattgtgcgg
     3421 ggagagatag attgatgctg ctggcgtaac gactcagctt catgtaccgg gcgttgtagg
     3481 agtttgccat tgctctctga tagctcgcgc gccagcgtca tgcagcctgc ccagccttca
     3541 cgttttgagg gcattggcga ttcccacata tccatccagc cgataacaat acgccgacca
     3601 tccttcgcta aaaagctttg tggtgcataa aagtcatgtc cgttatcaag ttcagtaaaa
     3661 tgcccggatt gtgcaaaaag tcgtcctggc gaccacattc cgggtattac gccactttga
     3721 aagcgatttc gataactgta tccctcggca ttcattccct gcggggaaaa catcagataa
     3781 tgctgatcgc caaggctgaa aaagtccgga cattcccaca tatagctttc acccgcatca
     3841 gcgtgggcca gtacgcgatc gaaagtccat tcacgcaatg aactgccgcg ataaagcagt
     3901 atctgccccg tgttacctgg gtctttcgcc ccgactacca tccaccatgt gtcggcttca
     3961 cgccacactt taggatcgcg gaagtgcatg attccttctg gtggagtgag gatcacaccc
     4021 tgtttctcga aatgaatacc atccagactg gtagccagac attgtacttc gcgaattgca
     4081 tcgtcattac ctgcaccatc gagccagacg tgtccggtgt agataagtga gaggacacca
     4141 ttgtcatcga cagcactacc tgaaaaacac ccgtctttgt cattctcgtc tcctggcgct
     4201 agcgcaatag gctcatgctg ccagtggatc atatcgtcgc tggtggcatg tccccagtgc
     4261 attggccccc agtgttcgct catcgggtga tgttgataaa acgcgtgata acgatcgtta
     4321 aaccagatca ggccgtttgg atcgttcatc cacccggcag gaggcgcgag gtgaaaatgg
     4381 ggatagaaag tgttacctcg gcgctcgtga agttttgcta gtgcgttttg cgccgcatgt
     4441 aatcgagatt gcgtcatttt tatcatcctg gttaagcaaa ttgggtgaat tgttaacgtt
     4501 aacttttata aaaataaagt cccttacttt cataaatgcg atgaatatca caaatgttaa
     4561 cgttaactat gacgttttgt gatcgaatat gcatgtttta ttaaatccat gacgattttg
     4621 cgaaaaagag gtttatcact atgcgtaagt cagatgaatt taagggaaaa aaatgtcagc
     4681 caaagtgtgg gttttagggg atgcggtcgt agatctcttg ccagaatcag acgggcggct
     4741 actgccttgt cctggcggtg cgccagctaa cgttgcggtg ggagtcgcca gattaggcgg
     4801 aacaagtgga tttataggtc gggtcggtga tgatcctttt ggtgcgttaa tgcaaagaac
     4861 gctgctaact gagggtgtcg atatcacgta tctgaagcaa gatgaatggc accggacatc
     4921 cacggtgctt gtcgatctga acgatcaagg agaacgttca tttacgttta tggtccgccc
     4981 cagtgccgat ctttttttag agacgacaga cttgccctgc tggcgacatg gcgaatggtt
     5041 acatctctgt tcaattgcgt tgtctgccga gccttcgcgt accagcgcat ttactgcgat
     5101 gacggcgatc cggcatgccg gaggttttgt cagcttcgat cccaatattc gtgaagatct
     5161 atggcaagac gagcatttgc tccgcttgtg tttgcggcag gcgctacaac tggcggatgt
     5221 cgtcaagctc tcggaagaag aatggcgact tatcagtgga aaaacacaga acgatcggga
     5281 tatatgcgcc ctggcaaaag agtatgagat cgccatgctg ttggtgacta aaggtgcaga
     5341 aggggtggtg gtctgttatc gaggacaagt tcaccatttt gctggaatgt ctgtgaattg
     5401 tgtcgatagc acgggggcgg gagatgcgtt cgttgccggg ttactcacag gtctgtcctc
     5461 tacggggtta tctacagatg agagagaaat gcgacgaatt atcgatctcg ctcaacgttg
     5521 cggagcgctt gcagtaacag cgaaaggggc aatgacagcg ctgccatgtc gacaagaact
     5581 ggaaagtgag aagtaaacgg cgaggccgct cttatctcta aataggacat gaatttttta
     5641 acgacaggca ggtaattatg gcactgaata ttccattcag aaatgcgtac tatcgttttg
     5701 catccagtta ctcatttctc ttttttattt cctggtcgct gtggtggtcg ttatacgcta
     5761 tttggctgaa aggacatcta ggattaacag ggacggaatt aggtacactt tattcggtca
     5821 accagtttac cagcattcta tttatgatgt tctacggcat cgttcaggat aaactcggtc
     5881 tgaagaaacc gctcatctgg tgtatgagtt tcattctggt cttgaccgga ccgtttatga
     5941 tttacgttta tgaaccgtta ctgcaaagca atttttctgt aggtctaatt ctgggggcgc
     6001 tcttttttgg cctggggtat ctggcgggat gcggtttgct tgacagcttc accgaaaaaa
     6061 tggcgcgaaa ttttcatttc gaatatggaa cagcgcgcgc ctggggatct tttggctatg
     6121 ctattggcgc gttctttgcc ggtatatttt ttagtatcag tccccatatc aacttctggt
     6181 tggtctcgct atttggcgct gtatttatga tgatcaacat gcgttttaaa gataaggatc
     6241 accagtgcat agcggcggat gcgggagggg taaaaaaaga ggattttatc gcagttttca
     6301 aggatcgaaa cttctgggtt tttgtcatat ttattgtggg gacgtggtct ttctataaca
     6361 tttttgatca acaactcttt cctgtctttt atgcaggttt attcgaatca cacgatgtag
     6421 gaacgcgcct gtatggttat ctcaactcat tccaggtggt actcgaagcg ctgtgcatgg
     6481 cgattattcc tttctttgtg aatcgggtag ggccaaaaaa tgcattactt atcggtgttg
     6541 tgattatggc gttgcgtatc ctttcctgcg cgctgttcgt taacccctgg attatttcat
     6601 tagtgaagct gttacatgcc attgaggttc cactttgtgt catatccgtc ttcaaataca
     6661 gcgtggcaaa ctttgataag cgcctgtcgt cgacgatctt tctgattggt tttcaaattg
     6721 ccagttcgct tgggattgtg ctgctttcaa cgccgactgg gatactcttt gaccacgcag
     6781 gctaccagac agttttcttc gcaatttcgg gtattgtctg cctgatgttg ctatttggca
     6841 ttttcttcct gagtaaaaaa cgcgagcaaa tagttatgga aacgcctgta ccttcagcaa
     6901 tatagacgta aactttttcc ggttgttgtc gatatctcca tatccctcaa ccggaaaata
     6961 ataatacaaa aatgcttagc cctgctaata atcgcctaat ccaaacgcct cattcatgtt
     7021 ctggtacagt cgctcaaatg tactccggat acgcggttcg ctgatttcca ggacattgtc
     7081 gtcattcagc gacctgtccc gtgtatcacg ggcctgcgaa ttcatcaagg aatgcattgc
     7141 agagtgaagt atcgagtcac gccatatttc gctatcagga ttctgtatga tggttacatc
     7201 gcccggccca gggctgttta gtcatcagcg ctttctgaca gtgctgagat ttcaacctgt
     7261 tgcagtaaaa atgagtagat atagggcaag tgtgctgcca aacccatctt ttacggggtg
     7321 aaggtagatt tggtttgaag ggtatctggt gtcccctgca ggaatcgaac ctgcaattag
     7381 cccttaggag gggctcgtta tatccattta actaagagga caatgcggca tgagtatacc
     7441 cgctaatgga ctacggggta agtacacagc cgctcgattg cttaaaccct cgccatttat
     7501 gccgggtttt tatcattttt cttaatgttt tccgcacgtt ctgctttttg acgtgcttct
     7561 actttgcgct tgttgctcat gtcgttacga atctgtgcat gactcattaa cgcgaagata
     7621 aaggtgccgc cgcagatgtt ccccgctaaa gtaggtagtg caaagggcca gatgaaatcg
     7681 ctccagtgca gcgtgccgtt aaacaccaga tagaggattt caacagaacc gacaacgatg
     7741 tgggtggtgt cacccagggc aataagccag gtcatcaata taatcaccac aatctttgcc
     7801 gcacccgctg caggaaacat ccataccata gtggcgatca gccagccgga aatgatcgcg
     7861 ttggcaaaca tctcgctggg ggtgttcttc atcacatcca tgccgatttt gacaaatgca
     7921 tcgcgagttt cttcattgaa gataggcata tattcaaatg cccacgcagc aatacctgtc
     7981 ccgagaatat tacccagcag cacgatgccc cataaccgca taagtaagcc gacgttgctc
     8041 attgtcggtt tttgcatgac gggtagtacc gcagttacgg tgttttcggt aaataattgc
     8101 tggcgggcca ttatgacgat aataaaacca aaggtataac cgagattctc cagcaagaag
     8161 ctgcccggca cgccttccag ttcgacatga aatatccctt ttgccagtag cgaagcgccc
     8221 atcgacaggc ccgccgcaat ggctgaccac agtagcgcca ttgcgtcgcg ttccagctct
     8281 ttttcaccat cctggcggat atgctcatga attgccatcg cccgggaggg gagtcggtct
     8341 tcatctattt ctattttttt accgcgctct ttttcttcgc tctcaacttc aatttcgtcg
     8401 ctgtgttgat caattttgtc gttgtccatg gtctcttctc ttgaattagc acgtatagct
     8461 aaagcgtagc ggcttttttg ctcgcaactg gcgggagtta ctctgaaaat gtagaaaagg
     8521 ccgcgtttgc ctttttctgt ttctattgaa tcaagtagcc tacagggcgg cgattaccag
     8581 gctatgatca aatcagcaaa tcagggcgtc tggacatcag ttgacgtgct gttacaatcg
     8641 cccacaccta aacaggcgga tacggtatcg ttccgtcatg gatggcaaac tgcataagcc
     8701 ataaaaaaac agggagacat ttatgaagct tcgcctgtcg gcgcttgctc tgggaactac
     8761 gcttctggtg gggtgtgcga gttccggtac agatcagcaa gggcgttctg acccgttaga
     8821 agggtttaac cgcactatgt acaacttcaa cttcaatgta ttagacccgt atattgttcg
     8881 accggtcgct gtcgcctggc gtgattatgt tccgcaacct gcgcgtaacg gtttgagcaa
     8941 ctttaccggc aaccttgaag aacctgcggt gatggttaac tacttcttgc agggcgaccc
     9001 ttatcagggg atggtccact ttacccgctt tttcctgaat accattttgg gaatgggcgg
     9061 ctttattgat gttgcaggga tggcaaaccc gaaactgcaa cggactgaac ctcaccgctt
     9121 cggtagtacg cttggtcatt atggtgtggg ttatgggccg tacgttcagt taccgttcta
     9181 cggtagcttc acgctgcgtg atgacggtgg tgatatggcg gatagtcttt acccggttct
     9241 ttcctggctg acctggccga tgtctgtggg taaatggacg cttgaaggga tagaaactcg
     9301 tgcgcagttg ttggattccg acggtctgct gcgtcagtcg tccgatcctt atattatggt
     9361 gcgtgaagcg tacttccagc gtcatgattt catcgctaat ggcggtgaac tcaaaccgca
     9421 ggaaaatccg aacgcgcaag cgattcagga tgatttaaaa gatatcgatt ctgaataaga
     9481 aacaaacaaa aaaggtgagt cgcaaaactc accttttttc ttattaagct aacgcgttat
     9541 cagaacgcgt agttaaagtt agtaccgaac agccaggctt taccttcaga ctcgaaggaa
     9601 taagctgccg ctccttcacc ttcggtgaat ttcactttct gaccatgcat ataagatgcg
     9661 ccaacatcaa tggatgcatc ttcattaaat gcgtaggtta acccagcact taaccacaaa
     9721 cgatcctggt caggaatgga aatagaacgt ttatctgccg gaaccgggct gtcgtcataa
     9781 gcgataccgg tacggaaggt ccagttttta tcataatagt aggttgtacc caacgcgata
     9841 cgatacgcgt cccggaatcc ttcatcttta tagaacagag tctgaccact atcgcctttc
     9901 gctttcaatt cctggaactg gctccaactg gtataggcca tgctgtagtg aattgcccac
     9961 tgcggcgcaa cacggttata accagaaatc tcccacatct caggcaggtt cagcgttaag
    10021 gatccatttt gggttgcccc catcgtgcca tacggtaagc ccatgttacc caaaatattg
    10081 ttcagatttg ggttaatgga gcttttgtaa tccccatcga agtcgatttt aacttctgaa
    10141 cgatatgtca gcccccaacg gttatcttta tccagttcat aaagaatacc cgcgttccag
    10201 ccaaagcccc attcgtcacc tttcaaatga gcaatttgag agtccggagt attaatacca
    10261 ttggttaatg aagcgggtac accctgggca atcaactgtt gagacaacaa tggccccagc
    10321 gaaccggcgt aacgctcaat ttttgccttg gcataaactg catcaaaacc aagaccaaaa
    10381 ctccagctat tgttaaggcg ataagccccg ctaaggttaa ggttgattgt ttgcagatcg
    10441 gttttaccgc catagatacc agcagcgtag ttgtcggtaa attccgtagc caggccatag
    10501 ttggaagtta ttgacgctcc ccaaccaaac tgatcgttaa taggcgcaac aaagtgcagg
    10561 ttaggtaccc atgctgtcgg tgcgatattt ttagcatttg cactctgacc ggttaaatca
    10621 gaatgtccgg taatatttac atccggatca atataaaccg cacccgcaga aaatgtcggg
    10681 cggtcaaaca tggtaatcaa tgcggggtta cggctaacgt tacctgcatc atcggcaatt
    10741 gcaccttccc ctgaataagc ccggcccagg ccagaggaag aaaattcgtt taactgaaag
    10801 cctgccgacc aggcctgggt ggagataagt gccactgcga ctgcgagagc agactttgta
    10861 aacagggttt tctggctcat gaccataacc tcaatgattt atttttatac aaactatgtt
    10921 acgtgctgta acaggagcgc gaagtgtagg gtctgaggta catcttagaa atcagaccag
    10981 tggcgagagt ataggtcgga ccagctggaa tgttgcaagt atgtttctat cttttttcag
    11041 atatgatcta agaaacgcga tccagctggc aaaattggga ggtggcgcac tcatggattc
    11101 gggtgaattt tgttttagat catttttaag tgtgatttcg gtcacttatc cgattttgca
    11161 gaattaacaa ctggagcagc actttccgga agcgtaaaat atccacaacg tttatctggc
    11221 accttgggtg cacatacaga ggaaatcaac gatgagtaaa tgtagtgctg atgaaacccc
    11281 ggtttgctgc tgtatggatg ttggcaccat tatggacaac tccgactgca ccgcgtctta
    11341 cagccgtgtg tttgcaaacc gcgcagaagc agaacaaacg ctggcggcgc tgactgaaaa
    11401 agcccgtagc gtggaatccg aaccgtgcaa aatcaccccg actttcactg aagagtctga
    11461 cggtgtgcgc ctggatatcg atttcacttt tgcctgcgaa gctgaaatgc tgatcttcca
    11521 gctgggtctg cgttaattcc tctcgggtac atacgcccct gtcagttgct ggcaggggcg
    11581 ttttcttttc tcttcttctt ttctctctgt gtcatagctc ccactttccc ttttctccgc
    11641 ttggctaaat gtaaaaaaat ggttaagact atgatcaggt cagaccactt tatttatttt
    11701 ttacagggga gtgttatggg tcaggtttta ccgctggtta cccgccaggg cgatcgtatc
    11761 gccattgtta gcggtttacg tacgcctttt gcccgacagg cgacggcttt tcatggcatt
    11821 cccgcggttg atttagggaa gatggtggta ggcgaactgc tggcacgcac cgagatcccc
    11881 gctgaagtga ttgaacaact ggtcttcggt caggtcgttc agatgcctga agcccccaac
    11941 attgcgcgtg aaattgttct cggtactgga atgaatgtgc ataccgatgc ttacagcgtt
    12001 agccgcgctt gcgctaccag tttccaggcg gttgcaaacg tcgcagaaag cctgatggcg
    12061 ggaactattc gagcggggat tgccggtggg gcagattcct cttccgtatt gccaattggc
    12121 gtcagtaaaa aactggcgcg cgtgctggtt gatgtcaaca aagcccgcac aatgagccag
    12181 cgcctgaaac ttttttctcg cttacgtttg cgtgacttaa tgccggtgcc tccggcagtg
    12241 gcggaatact ccactggatt acggatgggg gacaccgcag agcaaatggc gaaaacctac
    12301 ggcatcaccc gagaacagca agatgcactg gcgcaccgtt cgcatcagcg tgccgctcag
    12361 gcgtggtcag aaggtaaact caaagaagag gtgatgactg cctttatccc cccttataaa
    12421 caaccgcttg tcgaagacaa caatattcgc ggtaattcct ctcttgctga ttacgcaaag
    12481 ctgcgtccgg cgtttgatcg taaacacggg acggtaacgg cggcgaacag tacgccgctg
    12541 accgatggcg cggcagcggt gatcctgatg actgaatccc gggcgaaaga attagggctg
    12601 gtaccgctgg ggtatctgcg cagctacgca tttactgcga tagatgtctg gcaggacatg
    12661 ttgctcggtc cagcctggtc aacaccgctg gcgctggaac gtgccggatt gacgatgagc
    12721 gatctgacat tgatcgatat gcacgaagcc tttgccgccc agacgctggt caatattcag
    12781 ttgcttggca gcgaacgttt tgctcgtgac gtactggggc gtgcacatgc cactggcgaa
    12841 gtggacgaga gcaaatttaa cgtgcttggc ggttcgattg cttatgggca tcccttcgca
    12901 gcgaccggcg cgcggatgat tacccagaca ttgcatgaac ttcgccgtcg tggcggtgga
    12961 tttggtttag tgaccgcctg tgctgcgggt ggacttggcg cagcaatgat tctggaggcg
    13021 gaataatgga aatggcatca gcgtttactc ttaatgttcg tctggacaac attgctatca
    13081 tcaccatcga cgtaccgggt gagaaaatga ataccctgaa ggcagaattt gcctcgcagg
    13141 tacgcgccat tattaagcaa ctccgtgaaa acaaagagtt gcgtggcgtg gtgtttgtct
    13201 ccgcgaaacc agacaacttc attgctggcg cagacatcaa catgatcggc aactgcaaaa
    13261 cggcgcaaga agcggaagta ctggcgcggc aggggcaaca gttaatggcg gagattcatg
    13321 ctttgcccat tccggttatc gcggctattc atggcgcttg cctgggcggc gggctggagt
    13381 tggcgctggc gtgccacggt cgtgtttgta ctgacgatcc taaaacggtg ctcggtttgc
    13441 ctgaagtaca acttggattg ttacccggtt caggcggtac ccagcgttta ccgcgcctga
    13501 taggtgtcag cacagcatta gagatgatcc tcaccggaaa acaacttcgg gcgaaacagg
    13561 cggtaaagct ggggttggtg gatgacgtcg ttccgcactc catattgctg gaagccgctg
    13621 ttgagctggc aaagcaggat cgcccatctt cacgccctct acctgtacgc gagcgtattc
    13681 tggcggggcc gttaggtcgt gcgctgctgt tcaaaatggt cggtaagaaa acagaacaca
    13741 aaacgcaagg taactatccg gcgacagaac gcatcctgga ggttgtcgaa acgggattag
    13801 cgcagggcac cagcagcggc tatgacgctg aagcacgggc gtttggcgaa ctggcgatga
    13861 cgccgcaatc gcaggcgctg cgtagtatct tttttgccag tacggacgtg aagaaagatc
    13921 ccggcagtga tgcgccgcct gcgccattaa acagcgtggg gattctaggt ggtggcttga
    13981 tgggcggcgg tattgcttat gtcactgctt gtaaagcggg gcttccggtc agaattaaag
    14041 atatcaaccc gcagggcata aatcatgcgc tgaagtacag ttgggatcag cttgagggca
    14101 aagttcgccg tcgtcatctc aaagccagcg aacgtgacaa acagctggca ttaatctccg
    14161 gaacgacgga ctattgcggc tttgcccatc gcgatctgat tattgaagcg gtgtttgaaa
    14221 atctcgaatt aaaacaacag atggtggcgg aagtggagca aaattgcgct actcatacca
    14281 tctttgcttc gaacacgtca tctttaccga ttggcgatat cgccgctcac gccgtgcgac
    14341 ctgagcaagt tattggcctg catttcttca gtccggtgga aaaaatgccg ctggtggaga
    14401 ttattcctca tgcgagtaca tcggcgcaaa ccatcgccac cacggtaaaa ctggcaaaaa
    14461 aacagggcaa aacgccaatt gtcgtgcgtg acaaagccgg gttttacgtc aatcgcatct
    14521 tagcgcctta catcaatgaa gctatccgca tgttgaccga aggcgagcgt attgagcata
    14581 tcgatgccgc gctagtgaaa tttggttttc cggtaggccc aatccaactt ttggatgagg
    14641 taggaatcga caccgggact aaaattatgc ctgtactgga agccgcttat ggagaacgtt
    14701 ttagcgcgcc tgcaaatgtt gtttcttcaa ttttgaacga cgatcgcaaa ggcagaaaaa
    14761 atggccgggg tttctatctt tatggtcaga aagggcgtaa aagcaaaaaa caggtcgatc
    14821 ccgccattta cccgctcatt ggcgcacaag ggcaggggcg actctccgca ccgcaggttg
    14881 ctgaacggtg tgtgatgttg atgctgaatg aagcagtacg ttgtctggac gagcaggtta
    14941 tccgtagcgt gcgtgacggg gatataggct cggtatttgg cattggtttt ccgccatttc
    15001 tcggtggacc gttccgctat atcgattctc tcggcgcagg cgaagtggtt gcaataatgc
    15061 aacgactggc cacgcagtat ggttcccgtt ttaccccttg cgatcgttta gttgagatga
    15121 gcgagcgtgg ggaaagtttt tggaaaacaa ctgcaactga cctgcaataa gaaggtcaaa
    15181 gttatatgaa tccgcgctga atggcggagt gttgctcaaa atgtaaacgc atattgacta
    15241 tacttacgcc attgaggtaa aaaacagcgt ttcattcggt gaatggataa ggcacaatgc
    15301 cggccaccgt tttctttctc tggtttcaga tgaaagaaaa cgggcgaatc tggttaacaa
    15361 aagcggtgca atatgcaagt ttttatcatg cgtcacggcg acgcagccct cgatgccgcc
    15421 agtgattcgg ttcgtcctct gaccactaac ggttgtgacg aatctcgcct gatggcgaac
    15481 tggctgaaag gtcaaaaagt ggaaatcgaa cgcgttctgg tgagcccgtt cctgcgagcc
    15541 gagcaaacac tggaagaagt aggggattgt ctgaacctgc cttccagtgc agaagttctg
    15601 ccggagttaa cgccctgcgg cgatgttggt ctggtcggtg cctatttgca ggcgttgacc
    15661 aatgaaggtg ttgcttcggt actggtgatc tcccacttac cgttagtcgg gtatctggtt
    15721 gccgagttat gtccgggcga aacgccgcct atgtttacca cttcagcgat tgccagcgta
    15781 acgcttgatg aaagcggaaa aggccaattc aactggcaaa tgagcccttg taatttgaag
    15841 atggcaaaag caatttaagc aatcaatgcc
//
LOCUS       DADSYM010000115        15693 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2415
            SAMN08039579-rid11723173.denovo.116, whole genome shotgun sequence.
ACCESSION   DADSYM010000115 DADSYM010000000
VERSION     DADSYM010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039579
            Sequence Read Archive: SRR6362533
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15693)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15693)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980162.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 03:39:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,484
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 4,347
            CDSs (with protein)               :: 4,347
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 43 of 85
            Pseudo Genes (incomplete)         :: 37 of 85
            Pseudo Genes (internal stop)      :: 19 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15693
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039579-rid11723173.denovo.116"
                     /strain="LGE2415"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8302 N 95.9628 W"
                     /collection_date="2015-08-24"
     gene            16..855
                     /locus_tag="J1Y83_003202"
     CDS             16..855
                     /locus_tag="J1Y83_003202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001470439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBA7631323.1"
                     /translation="MKSGSTTQASVMLALSQSAVSAALTDLEGQLGVQLFDRVGKRLV
                     VNEHGRLLYPRALALLEQAVEIEQLFREDNGAIRIYASSTIGNYILPAVIARYRHDYP
                     QLPIELSVGNSQDVMQAVLDFRVDIGFIEGPCHSTEIISEPWLEDELVVFAAPTSPLA
                     RGPVTLEQLAAAPWILRERGSGTREIVDYLLLSHLPKFEMAMELGNSEAIKHAVRHGL
                     GISCLSRRVIEDQLQAGTLSEVAVPLPRLMRTLWRIHHRQKHLSNALRRFLDYCDPAN
                     VPR"
     gene            1060..2529
                     /gene="lysP"
                     /locus_tag="J1Y83_003203"
     CDS             1060..2529
                     /gene="lysP"
                     /locus_tag="J1Y83_003203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine-specific permease"
                     /protein_id="HBA7631324.1"
                     /translation="MGSETKTTEAPGLRRELKARHLTMIAIGGSIGTGLFVASGATIS
                     QAGPGGALLSYMLIGLMVYFLMTSLGELAAYMPVSGSFATYGQNYVEEGFGFALGWNY
                     WYNWAVTIAVDLVAAQLVMSWWFPDTPGWIWSALFLGVIFLLNYISVRGFGEAEYWFS
                     LIKVTTVIVFIIVGVLMIIGIFKGAQPAGWSNWTIGEAPFAGGFAAMIGVAMIVGFSF
                     QGTELIGIAAGESEDPAKNIPRAVRQVFWRILLFYVFAILIISLIIPYTDPSLLRNDV
                     KDISVSPFTLVFQHAGLLSAAAVMNAVILTAVLSAGNSGMYASTRMLYTLACDGKAPR
                     IFAKLSRGGVPRNALYATTVIAGLCFLTSMFGNQTVYLWLLNTSGMTGFIAWLGIAIS
                     HYRFRRGYVLQGHDINDLPYRSGFFPLGPIFAFILCLIITLGQNYEAFLKDTIDWGGV
                     AATYIGIPLFLIIWFGYKLIKGTHFVRYSEMKFPQNDKK"
     gene            2821..4812
                     /gene="cirA"
                     /locus_tag="J1Y83_003204"
     CDS             2821..4812
                     /gene="cirA"
                     /locus_tag="J1Y83_003204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catecholate siderophore receptor CirA"
                     /protein_id="HBA7631325.1"
                     /translation="MFRLNPFVRVGLCLSAISCAWPVLAVDDDGETMVVTASSVEQNL
                     KDAPASISVITQEDLQRKPVQNLKDVLKEVPGVQLTNEGDNRKGVSIRGLDSSYTLIL
                     VDGKRVNSRNAVFRHNDFDLNWIPVDSIERIEVVRGPMSSLYGSDALGGVVNIITKKI
                     GQKWSGTVTVDTTVQEHRDRGDTYNGQFFTSGPLIDGVLGMKAYGSLAKREKDDPQNS
                     TTTDTGETPRIEGFSSRDGNVEFAWTPNQNHDFTAGYGFDRQDRDSDSLDKNRLERQN
                     YSVSHNGRWDYGTSELKYYGEKVENKNPGNSSPITSESNTVDGKYTLPLTAINQFLTV
                     GGEWRHDKLSDAVNLTGGTSSKTSASQYALFVEDEWRIFEPLALTTGVRMDDHETYGE
                     HWSPRAYLVYNATDTVTVKGGWATAFKAPSLLQLSPDWTSNSCRGACKIVGSPDLKPE
                     TSESWELGLYYMGEEGWLEGVESSVTVFRNDVKDRISISRTSDVNAAPGYQNFVGFET
                     GANGRRIPVFSYYNVNKARIQGVETELKIPFNDEWKLSINYTYNDGRDVSNGENKPLS
                     DLPFHTANGTLDWKPLALEDWSFYVSGHYTGQKRADSATAKTPGGYTIWNTGAAWQVT
                     KDVKLRAGVLNLGDKDLSRDDYSYNEDGRRYFMAVDYRF"
     gene            complement(4844..5680)
                     /gene="yeiG"
                     /locus_tag="J1Y83_003205"
     CDS             complement(4844..5680)
                     /gene="yeiG"
                     /locus_tag="J1Y83_003205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-formylglutathione hydrolase"
                     /protein_id="HBA7631326.1"
                     /translation="MEMLEEHRCFEGWQQRWRHDSSTLNCPMTFSIFLPPPRDHTPPP
                     VLYWLSGLTCNDENFTTKAGAQRVAAELGIVLVMPDTSPRGEQVANDDGYDLGQGAGF
                     YLNATQPPWATHYRMYDYLRDELPALVQSRFNVSDRCAISGHSMGGHGALIMALKNPG
                     KYTSVSAFAPIVNPCSVPWGIKAFSTYLGEDKNAWLEWDSCALMYASNAQDAIPTLID
                     QGDNDQFLADQLQPAVLAEAARQKAWPMTLRIQPGYDHSYYFIASFIEDHLRFHAQYL
                     LK"
     gene            5938..6606
                     /gene="folE"
                     /locus_tag="J1Y83_003206"
     CDS             5938..6606
                     /gene="folE"
                     /locus_tag="J1Y83_003206"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HBA7631327.1"
                     /translation="MPSLSKEAALVHEALVARGLETPLRPPVHEMDNETRKSLIAGHM
                     TEIMQLLNLDLADDSLMETPHRIAKMYVDEIFSGLDYANFPKITLIENKMKVDEMVTV
                     RDITLTSTCEHHFVTIDGKATVAYIPKDSVIGLSKINRIVQFFAQRPQVQERLTQQIL
                     IALQTLLGTNNVAVSIDAVHYCVKARGIRDATSATTTTSLGGLFKSSQNTRHEFLRAV
                     RHHN"
     gene            6623..7780
                     /locus_tag="J1Y83_003207"
     CDS             6623..7780
                     /locus_tag="J1Y83_003207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF418 family protein"
                     /protein_id="HBA7631328.1"
                     /translation="MERNVTLDFVRGVAVLGILLLNISAFGLPKAAYLNPAWYGAITP
                     QDAWTWAFLDLIGQVKFLTLFALLFGAGLQMLLPRGRRWIQSRLTLLVLLGFIHGLLF
                     WDGDILLAYGLVGLICWRLVRDAPSVKSLFNTGVMLYLVGLGVLLLLGLISDSQTSRA
                     WTPDASSILYEKHWKLHGGVEAIGNRADGVGNSLLALGAQYGWQLAGMMLIGAALMRS
                     GWLKGQFSLRHYRRTGFVLVAIGVIINLPAIALQWRLDWAYRWCAFLLQMPRELSAPF
                     QAIGYASLFYGFWPQLSRFKLVLAIACVGRMALTNYLLQTLICTTLFYHLGLFMQFDR
                     LELLAFVIPVWLANIFFSVIWLRYFRQGPVEWLWRQLTLRAAGPTISKTSR"
     gene            7922..8962
                     /gene="galS"
                     /locus_tag="J1Y83_003208"
     CDS             7922..8962
                     /gene="galS"
                     /locus_tag="J1Y83_003208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708050.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator GalS"
                     /protein_id="HBA7631329.1"
                     /translation="MITIRDVARQAGVSVATVSRVLNNSTLVSADTREAVMKAVSELD
                     YRPNANAQALATQVSDTIGVVVMDVSDAFFGALVKAVDLVAQQHQKYVLIGNSYHEAE
                     KERHAIEVLIRQRCNALIVHSKALSDDELAQFMDNIPGMVLINRVVPGYAHRCVCLDN
                     LSGARMATRMLLNNGHQRIGYLSSSHGIEDDAMRKAGWMSALKEQDIIPPESWIGTGT
                     PDMPGGEAAMVELLGRNLQLTAVFAYNDNMAAGALTALKDNGIAIPLHLSIIGFDDIP
                     IARYTDPQLTTVRYPIASMAKLATELALQGAADNIDPRASHCFMPTLVRRHSVATRQN
                     AAAITNSTNQAM"
     gene            9242..10240
                     /gene="mglB"
                     /locus_tag="J1Y83_003209"
     CDS             9242..10240
                     /gene="mglB"
                     /locus_tag="J1Y83_003209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/glucose ABC transporter
                     substrate-binding protein MglB"
                     /protein_id="HBA7631330.1"
                     /translation="MNKKVLTLSAVMASMLFGAAAHAADTRIGVTIYKYDDNFMSVVR
                     KAIEQDAKAAPDVQLLMNDSQNDQSKQNDQIDVLLAKGVKALAINLVDPAAAGTVIEK
                     ARGQNVPVVFFNKEPSRKALDSYDKAYYVGTDSKESGIIQGDLIAKHWAANQGWDLNK
                     DGQIQFVLLKGEPGHPDAEARTTYVIKELNDKGIKTEQLQLDTAMWDTAQAKDKMDAW
                     LSGPNANKIEVVIANNDAMAMGAVEALKAHNKSSIPVFGVDALPEALALVKSGALAGT
                     VLNDANNQAKATFDLAKNLADGKGAADGTNWKIDNKVVRVPYVGVDKDNLAEFSKK"
     gene            10301..11821
                     /gene="mglA"
                     /locus_tag="J1Y83_003210"
     CDS             10301..11821
                     /gene="mglA"
                     /locus_tag="J1Y83_003210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/methyl galactoside ABC transporter
                     ATP-binding protein MglA"
                     /protein_id="HBA7631331.1"
                     /translation="MVSSTTPSSGEYLLEMSGINKSFPGVKALDNVNLKVRPHSIHAL
                     MGENGAGKSTLLKCLFGIYQKDSGTILFQGKEIDFHSAKEALENGISMVHQELNLVLQ
                     RSVMDNMWLGRYPTKGMFVDQDKMYRETKAIFDELDIDIDPRARVGTLSVSQMQMIEI
                     AKAFSYNAKIVIMDEPTSSLTEKEVNHLFTIIRKLKERGCGIVYISHKMEEIFQLCDE
                     VTVLRDGQWIATEPLAGLTMDKIIAMMVGRSLNQRFPDKENKPGEVILEVRNLTSLRQ
                     PSIRDVSFDLHKGEILGIAGLVGAKRTDIVETLFGIREKSAGTITLHGKKINNHNANE
                     AINHGFALVTEERRSTGIYAYLDIGFNSLISNIRNYKNKVGLLDNSRMKSDTQWVIDS
                     MRVKTPGHRTQIGSLSGGNQQKVIIGRWLLTQPEILMLDEPTRGIDVGAKFEIYQLIA
                     ELAKKGKGIIIISSEMPELLGITDRILVMSNGLVSGIVDTKTTTQSEILRLASLHL"
     gene            11837..12847
                     /gene="mglC"
                     /locus_tag="J1Y83_003211"
     CDS             11837..12847
                     /gene="mglC"
                     /locus_tag="J1Y83_003211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/methyl galactoside ABC transporter
                     permease MglC"
                     /protein_id="HBA7631332.1"
                     /translation="MSALNKKSFLTYLKEGGIYVVLLVLLAIIIFQDPTFLSLLNLSN
                     ILTQSSVRIIIALGVAGLIVTQGTDLSAGRQVGLAAVVAATLLQSMDNANKVFPEMAT
                     MPIALVILIVCAIGAVIGLINGLIIAYLNVTPFITTLGTMIIVYGINSLYYDFVGASP
                     ISGFDSGFSTFAQGFIALGSFRLSYITFYALIAVVFVWVLWNKTRFGKNIFAIGGNPE
                     AAKVSGVNVGLNLLMIYALSGVFYAFGGMLEAGRIGSATNNLGFMYELDAIAACVVGG
                     VSFSGGVGTVIGVVTGVIIFTVINYGLTYIGVNPYWQYIIKGAIIIFAVALDSLKYAR
                     KK"
     gene            complement(13090..14325)
                     /gene="preA"
                     /locus_tag="J1Y83_003212"
     CDS             complement(13090..14325)
                     /gene="preA"
                     /locus_tag="J1Y83_003212"
                     /EC_number="1.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent dihydropyrimidine dehydrogenase
                     subunit PreA"
                     /protein_id="HBA7631333.1"
                     /translation="MLTKDLSITFCGVKFPNPFCLSSSPVGNCYEMCAKAYDTGWGGV
                     VFKTIGFFIANEVSPRFDHLVKEDTGFIGFKNMEQIAEHPLEENLAALRRLKEDYPDK
                     VLIASIMGENEQQWEELARLVQEAGADMIECNFSCPQMTSHAMGSDVGQSPELVEKYC
                     RAVKRGSTLPMLAKMTPNIGDMCEVALAAKRGGADGIAAINTVKSITNIDLNQKIGMP
                     IVNGKSSISGYSGKAVKPIALRFIQQMRTHPELRDFPISGIGGIETWEDAAEFLLLGA
                     ATLQVTTGIMQYGYRIVEDMASGLSHYLADQGFDSLQEMVGLANHNIVPAEDLDRSYI
                     VYPHINLDKCVGCGRCYISCYDGGHQAMEWSEKTRTPHCNTEKCVGCLLCGHVCPVGC
                     IDLGEVKFKKGEKEHPVTL"
     gene            complement(14319..15557)
                     /locus_tag="J1Y83_003213"
     CDS             complement(14319..15557)
                     /locus_tag="J1Y83_003213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HBA7631334.1"
                     /translation="MPQQNYLDELTPAFTPLLAIKEASRCLLCHDAPCSQACPAQTDP
                     GKFIRSIYFRNFKGAAETIRENNALGAVCARVCPTEKLCQSGCTRAGVDAPIDIGRLQ
                     RFVTDFEQQTGMEIYQPGTKTLGKVAIIGAGPAGLQASVTLTNQGYDVTIYEKEAQPG
                     GWLRNGIPQFRLPQSVLDAEIARIEKMGVTIKCNNEIGKTLTLEQLKAENRAVLVTVG
                     LSSGSGLSLFEHSDVEIAVDFLQRARQAQGDISIPQSALIIGGGDVAMDVASTLKVLG
                     CQAVTCVAREELDEFPASEKEFTSARELGVSIIDGFTPVAVEGNKVTFKHVRLPGELT
                     MAADKIILAVGQHARLDAFAELEPQRNTIETQHYQTRDPQVFAAGDIVEGDKTVVYAV
                     KTGKEAAGAIHHYLEGACSC"
BASE COUNT         3762 a         3836 c         4107 g         3988 t
ORIGIN      
        1 gtttttgcag aagtattgaa aagtggatca accacccagg cgtcggtgat gctggcgttg
       61 tcgcaatcag cagtgagcgc agccttgacc gacctggaag ggcagcttgg cgttcaactg
      121 tttgatcgcg tggggaaaag actggttgtt aatgaacacg ggcggctgct ctatccgcga
      181 gcgttggcac tgcttgaaca ggcggttgaa atcgaacaac tgtttcgcga agacaacggc
      241 gcgattcgta tctatgccag tagtaccatc ggtaactaca ttctgcctgc agttatcgcc
      301 cgttatcgcc atgattatcc gcagttgccg attgaactta gcgttgggaa tagccaggac
      361 gtgatgcaag cggtgctgga tttccgcgtt gatattggct ttattgaagg accgtgccac
      421 agcactgaaa tcatttctga accgtggctg gaagacgagc tggtggtttt cgccgcgccg
      481 acttcgccgt tggctcgtgg tccggtcacc ttagaacagc tggccgctgc gccgtggatc
      541 ctgcgtgaac gcggttccgg cacgcgggag attgtcgatt atctgttgct atcacattta
      601 ccgaagtttg agatggcgat ggaattaggt aactccgagg caatcaaaca tgcggtgcgt
      661 catgggttgg gaattagttg cctgtcgcga cgggtgattg aagatcaatt gcaggcaggc
      721 acattaagtg aagttgcggt ccctctgccg cgcctgatgc gtacgttgtg gcgtatacat
      781 catcggcaaa aacacctttc caacgcgcta cggcgctttc tggactattg cgatcccgcg
      841 aatgtaccgc gttaaggtgc tgtacaagaa catgctggtg ctgtgtcgat ttcgtgacgc
      901 agcgccctca gcatgcattc gccagaaaag agattggctg ctttacttat aatccctggg
      961 cgatcatgaa ggtgtcttat aaccgtgtat ttctgccgga aggattgcca atcgtctgct
     1021 acaatcgcgc ctcattttta agatggatag catttttgta tgggttccga aactaaaact
     1081 acagaagcgc cgggcttacg ccgtgaattg aaggcgcgtc acctgacgat gattgccatt
     1141 ggcggttcca tcggtacagg tctttttgtt gcctctggcg caacgatttc tcaggcaggt
     1201 ccgggcgggg cattgctctc gtatatgctg attggcctga tggtttactt cctgatgacc
     1261 agtctcggtg aactggctgc atatatgccg gtttccggtt cgtttgccac ttacggtcag
     1321 aactatgttg aagaaggctt tggcttcgcg ctgggctgga actactggta caactgggcg
     1381 gtgactattg ccgttgacct ggttgcagca cagctggtca tgagctggtg gttcccggat
     1441 acaccgggct ggatctggag tgcgttgttc ctcggcgtta tcttcctgtt gaactacatc
     1501 tctgttcgcg gctttggtga agcggaatac tggttctcac tgatcaaagt cacgacagtt
     1561 attgtcttta tcatcgttgg cgtgctgatg attatcggta tcttcaaagg cgcgcagcct
     1621 gcaggctgga gcaactggac aatcggcgaa gcgccgtttg ctggtggttt tgcggcgatg
     1681 atcggcgtag ctatgattgt cggcttctct ttccagggaa ccgagctgat cggtattgct
     1741 gcaggcgagt ccgaagatcc ggcgaaaaac attccacgcg cggtacgtca ggtattctgg
     1801 cgaatcctgt tgttctatgt gttcgcgatc ctgattatca gcctgattat tccgtatacc
     1861 gatccgagcc tgctgcgtaa cgatgttaaa gacatcagcg tcagtccgtt caccctggtg
     1921 ttccagcacg cgggtctgct ctctgcggcg gcggtgatga acgcagttat tctgacggcg
     1981 gtgctgtcag caggtaactc cggtatgtat gcgtctactc gtatgctgta taccctggcg
     2041 tgtgacggta aagcgccgcg cattttcgct aaactgtcgc gtggtggcgt gccgcgtaat
     2101 gcgctgtatg cgacgacggt gattgccggt ttgtgcttcc tgacctccat gtttggcaac
     2161 cagacggtat acctgtggct gctgaacacc tccgggatga cgggttttat cgcctggctg
     2221 gggattgcca ttagccacta tcgtttccgt cgcgggtacg tattgcaggg acacgacatt
     2281 aacgatctgc cgtaccgttc cggtttcttc ccactggggc cgatcttcgc attcattctg
     2341 tgtctgatta tcactttggg ccagaactac gaagcgttcc tgaaagatac cattgactgg
     2401 ggcggcgtag cggcaacgta tattggtatc ccgctgttcc tgattatttg gttcggctac
     2461 aagctgatta aaggaactca cttcgtacgc tacagcgaaa tgaagttccc gcagaacgat
     2521 aagaaataag tttcctccct tccttgctaa gccctctcaa ccgagagggc tttttcaatt
     2581 ccatttccct gacaaatcat gcggatataa aatttaacat ttggattgat aattgttatc
     2641 gtttgcatta tcgttacgcc gcaatcaaaa aaggctgaca aatcagaggc tgttccggct
     2701 ttctgggatg atcacctgca tacaaataag tccaccgcga tgctgccgta cgcaagggga
     2761 cgtaaagaag atgtgagcga tacccatttt attttcgtag ttacctcatg gagatatgga
     2821 atgtttaggt tgaacccttt cgtacgggtc gggctgtgtt tgtccgctat ttcttgtgca
     2881 tggcctgtgt tagcggtcga tgatgatggc gaaacgatgg ttgtcactgc atcttccgtt
     2941 gaacaaaacc ttaaagatgc tcccgccagt atcagcgtca ttacccagga agacctgcag
     3001 cgaaaaccgg tacagaatct gaaggatgtc ctcaaagaag tgcctggcgt acaactgacg
     3061 aacgaagggg ataaccgtaa gggcgtaagt attcgtggtc tggacagcag ctacaccctg
     3121 attcttgtcg acggtaaacg cgttaactcc cgcaatgccg tcttccgcca caatgatttc
     3181 gatctgaact ggatcccggt cgattccatc gaacgtattg aagtggtccg tggcccgatg
     3241 tcgtcgctgt acggttccga tgcgctcggc ggtgtagtga atatcatcac caaaaaaatc
     3301 ggtcagaaat ggtcgggcac cgttaccgtc gataccaccg ttcaggaaca tcgcgatcgc
     3361 ggtgatacct ataacggtca attctttacc agcggaccat taattgacgg cgtgctggga
     3421 atgaaagctt acggcagcct ggcaaaacgt gaaaaggatg acccgcaaaa ctcaacgacc
     3481 accgataccg gagaaacgcc gcgtattgaa ggattctcca gccgcgacgg caatgtcgaa
     3541 tttgcctgga caccgaatca aaatcacgat tttactgccg gatacggttt cgaccgtcag
     3601 gatcgtgatt ccgactcgct ggacaaaaac cgcctggaac gccagaacta ctccgtcagc
     3661 cataatgggc gttgggatta cggcaccagc gaactgaaat actacggtga gaaagtcgag
     3721 aacaaaaacc ctggcaacag cagcccgata acttccgaaa gcaatacggt cgacggcaaa
     3781 tacacgttgc cgctgacggc gattaatcag tttctcacgg ttggcggtga atggcgtcac
     3841 gacaaactta gcgatgcggt gaacctgacc gggggaacca gctccaaaac gtctgccagc
     3901 cagtacgcgc tgtttgtgga agatgaatgg cggatcttcg agccgctggc gctgacgacc
     3961 ggcgtgcgta tggacgatca cgaaacctac ggtgaacact ggagtccgcg tgcctacctg
     4021 gtttataacg ccaccgacac cgtaacggtg aaagggggct gggcgacggc atttaaagcc
     4081 ccttctctgt tgcaacttag ccctgactgg acgagcaatt cctgccgtgg cgcatgtaag
     4141 attgtgggta gcccggatct gaaaccagaa accagcgaaa gttgggagct ggggctttac
     4201 tacatgggtg aagaaggctg gctggaaggg gttgaatcca gcgttaccgt tttccgtaac
     4261 gatgtgaaag atcgtatcag cattagccgt acgtctgacg tcaatgctgc accgggctac
     4321 caaaactttg tcggttttga gacgggcgct aacggacggc gcataccggt atttagctac
     4381 tacaacgtta acaaagctcg tattcagggc gtggaaaccg aactgaaaat tccgttcaac
     4441 gatgaatgga aactgtcgat caactacacc tacaacgatg gtcgtgatgt cagcaacggc
     4501 gaaaacaaac cgctatccga tctgccgttc catactgcta acggtacgct ggactggaaa
     4561 ccgctggcgc tggaagactg gtcattctat gtttctggtc actataccgg gcagaaacgc
     4621 gccgacagcg cgacggctaa aacaccgggc ggttatacca tctggaatac cggcgcggcc
     4681 tggcaggtga ctaaagacgt caaactgcgc gcaggcgtgc tgaaccttgg cgacaaggat
     4741 ctcagtcgtg acgactacag ctataacgaa gacggacgtc gttactttat ggcagtggat
     4801 tatcgcttct gatgagaaga tgcccggcga accgggcgga ctttcacttc agtaaatact
     4861 gcgcatggaa gcgcaggtga tcctctataa aagaggcgat gaagtagtaa ctgtgatcat
     4921 atcccggctg aatacgcagc gtcatcggcc acgctttctg gcgcgcggct tctgccagta
     4981 ctgcaggttg cagttggtca gcaagaaact gatcattatc accctgatca ataagcgtcg
     5041 ggattgcatc ctgcgcgtta ctggcataca tcaacgcgca actgtcccat tccagccatg
     5101 catttttgtc ctcacctaaa taagtgctaa acgctttgat tccccacggg acgctgcacg
     5161 gattcacaat tggcgcaaag gcggaaacgc tggtgtattt acccggattt ttcagcgcca
     5221 taatcagcgc accgtgacca cccatggaat gaccgctaat agcgcagcgg tcgctgacat
     5281 taaatcgcga ctgaaccagc gccggtaatt catcgcgcag ataatcatac atccggtaat
     5341 gcgtcgccca cggtggttgc gtggcgttaa gataaaaccc cgcgccctgg cctaaatcgt
     5401 agccatcgtc gttggcaacc tgttcgccgc gcgggctggt gtctggcatc accagtacaa
     5461 tccccagttc cgccgccacg cgctgggcac ccgccttggt ggtgaagttc tcgtcattgc
     5521 aggttaatcc ggaaagccag tacagcactg gtggcggggt gtgatcacga ggtggtggga
     5581 gaaagatact gaacgtcatc gggcagttta aggtactgga gtcgtgtcgc cagcgttgct
     5641 gccagccttc aaaacagcgg tgctcttcga gcatttccat gcgtggctcc ttgttgtgtt
     5701 gtttgcaatt tcttcatcat accgtttatt caatgtgctg tgagtaactt tcacttccgt
     5761 atttgcataa cgatgtttta acatctgctg atgaaaggca gcggcaatta caataattat
     5821 cgctgtgaat actggattat gtgcgccgcc tcacgcacaa taatcagact gtaaatcagc
     5881 ttaataactt tgcccccacg cagggcggag gcgtcacacc tgcaggagaa atcataaatg
     5941 ccatcactca gtaaagaagc ggccctggtt catgaagcgt tagttgcgcg aggactggaa
     6001 acaccgctgc gcccgcccgt gcatgaaatg gataacgaaa cgcgcaaaag ccttattgct
     6061 ggtcatatga ccgaaatcat gcagctgctg aatctcgacc tggctgatga cagtttgatg
     6121 gaaacgccgc atcgcatcgc taaaatgtat gtcgatgaaa ttttctccgg tctggattac
     6181 gccaatttcc cgaaaatcac cctcattgaa aacaaaatga aggtcgatga aatggtcacc
     6241 gtgcgcgata tcactctgac cagcacctgt gaacaccatt ttgttaccat cgatggcaaa
     6301 gcgacggtgg cctatatccc gaaagattcg gtcatcggtc tgtcaaaaat taaccgtatt
     6361 gtgcagttct ttgcccagcg tccgcaggta caggaacgtc tgacgcagca aattctcatt
     6421 gcgctacaaa cgctgctggg caccaataat gtggctgtct ctatcgacgc ggtgcattac
     6481 tgcgtgaagg cgcgtggcat ccgcgatgca accagtgcca cgacaacgac ctctcttggt
     6541 ggattgttca aatccagtca gaatacgcgc cacgagtttc tgcgcgctgt gcgtcatcac
     6601 aactgataaa aaggcaggaa ccgtggagcg caacgtcacg ctcgattttg ttcgcggcgt
     6661 cgccgttctg gggatcctgc tattaaacat cagcgccttt gggctaccaa aggcggctta
     6721 tctcaatccc gcctggtacg gcgctattac gccacaggat gcatggacct gggcatttct
     6781 cgatctcatc ggccaggtga aattcctcac gctttttgcg ctgttgtttg gtgcgggcct
     6841 gcaaatgttg ctgccccgtg gcagacgatg gatccagtcg cggttaacgc tgttagtctt
     6901 gctgggcttt attcacggtt tattgttctg ggacggcgat attctgctgg cttacgggct
     6961 ggtgggctta atctgctggc ggctggtgcg cgatgcgcca tcggtaaaaa gcctgtttaa
     7021 taccggcgtc atgctttatc tggtggggct tggcgttttg ctgttattgg ggttgatttc
     7081 cgatagccag accagccgcg cctggacacc tgatgcatcg tctattttgt atgaaaagca
     7141 ctggaaactt cacggtggtg ttgaagcgat cggtaatcgt gccgatggtg ttggcaacag
     7201 tttactggca ctgggcgcac agtatggctg gcaactggca gggatgatgc tcattggtgc
     7261 cgcattgatg cgcagtggct ggctgaaagg gcagttcagc ttacgtcact atcgtcgtac
     7321 tggttttgtg ctggtggcga ttggggtgat cattaacctt cctgccatcg ccctgcaatg
     7381 gcgactggac tgggcgtatc gttggtgcgc cttcttactt cagatgccgc gggaactgag
     7441 tgcgccgttt caggcgattg gctatgcgtc gctgttttat ggcttctggc cgcaattaag
     7501 ccgctttaag ctggtgctgg caatcgcctg tgtcggacgg atggcactga ccaactatct
     7561 attgcaaacg ctgatttgta ctacgctttt ttaccacctc ggtttgttta tgcagtttga
     7621 ccgtctggag ctgctggcgt ttgttattcc ggtatggttg gcgaatatct tcttctctgt
     7681 tatctggctg cgttacttcc gccaggggcc ggtggaatgg ctctggcgtc agttaacttt
     7741 gcgtgctgcc ggaccgacaa tatctaaaac atcaagataa cgatctggat cacattcgtt
     7801 aacaaaacgg ctgtaaccgt ttccattgct gtgactcgat tcacgaagtc ctgtattcag
     7861 tgctgacaaa atagccgcca gcaagcagtc atttactgca atctcataac aggtagtgaa
     7921 tatgatcacc attcgtgatg tagcgcgtca ggctggcgtc tctgtggcaa cggtttcccg
     7981 ggtgctcaat aacagcacgc tggtcagtgc cgatacgcgt gaagcagtaa tgaaagccgt
     8041 gagtgagctg gattatcggc caaacgccaa tgcccaggcg ctggcaactc aggttagcga
     8101 taccattggc gtggtggtga tggacgtttc tgatgcgttt ttcggcgcgc tggtaaaagc
     8161 ggtggatctg gtcgcccagc agcatcagaa atacgtgcta atcggcaata gctatcatga
     8221 agcggaaaaa gagcgtcacg ccattgaggt gttaattcgc cagcgttgta atgcgttgat
     8281 tgttcactca aaagcattga gtgacgatga actggcacaa tttatggata acattcccgg
     8341 tatggtgtta atcaaccgcg ttgtgccggg gtacgcccat cgttgcgttt gcctggataa
     8401 tctcagcggt gcccgaatgg cgacgcgcat gttgctgaat aacggtcatc aacgtattgg
     8461 ttatctttct tccagccacg gcattgaaga tgacgccatg cgtaaagcag gctggatgag
     8521 tgcgttgaaa gagcaggata ttattccgcc ggaaagctgg attggcactg gtacgccgga
     8581 catgccgggc ggtgaggcgg cgatggttga actgctgggg cgcaatctac aactaaccgc
     8641 tgtatttgct tataacgaca atatggccgc tggcgcactg acagcattaa aagataatgg
     8701 cattgcgatt ccgttacatc tctcaatcat tggtttcgat gatattccca tcgcccgtta
     8761 caccgacccg caattaacga ctgtgcgtta tcccattgct tcaatggcta aattagctac
     8821 cgaactggcc ttgcaggggg cagcagacaa tattgatcct cgtgccagcc actgttttat
     8881 gccgacgtta gtgcgtcgcc attctgtcgc aacacgccag aatgcggcgg cgatcactaa
     8941 ctcaacaaat caggcgatgt aaccgctttc aatctgtgag tgatttcaca gtatcttaac
     9001 aatgtgatgg ctatgattgc accgttttaa cgttgtaacc cgtatgtaac agtgaataat
     9061 cactttcacc gaggtaatag cgtcataaca acaattaaag ccgttttctg gagcgttacc
     9121 gggcatggaa gaacgaattt taaaaagtga gcttcggcgt tcagtaacac ttcattaact
     9181 ctactgcccc gccgagcatt tatctcaagc actaccctgc ataagaaaaa ccggagatac
     9241 catgaataag aaggtgttaa ccctgtctgc tgtgatggcc agcatgttat tcggtgccgc
     9301 tgcacacgct gctgatactc gcattggtgt aacaatctat aagtacgacg ataactttat
     9361 gtctgtagtg cgcaaggcta ttgagcaaga tgcgaaagcc gcgccagatg ttcagctgct
     9421 gatgaatgat tctcagaatg accagtccaa gcagaacgat cagatcgacg tattgctggc
     9481 gaaaggggtg aaggcactgg caatcaacct ggttgacccg gcagctgcgg gtacggtgat
     9541 tgagaaagcg cgtgggcaaa acgtgccggt ggttttcttc aacaaagaac cgtctcgtaa
     9601 ggcgctggat agctacgaca aagcctacta cgttggcact gactccaaag agtccggcat
     9661 tattcagggc gatttgattg ctaaacactg ggcggcgaat cagggttggg atctgaacaa
     9721 agacggtcag attcaattcg tactgctgaa aggtgagccg gggcatccgg atgcagaagc
     9781 acgtaccact tacgtgatta aagaattgaa cgacaaaggc attaaaactg aacagttaca
     9841 gttagatacc gctatgtggg ataccgctca ggcgaaagat aagatggacg cctggctgtc
     9901 tggcccgaac gccaacaaaa tcgaggtggt tatcgccaac aacgacgcga tggcaatggg
     9961 cgcggtagaa gcactgaaag cacacaacaa gtccagcatt ccggtgtttg gcgtcgatgc
    10021 gctgccagaa gcgctggcgc tggtgaaatc cggtgcactg gcgggcaccg tactgaacga
    10081 tgctaacaac caggcgaaag cgacctttga tctggcgaaa aacctggccg atggcaaagg
    10141 tgcggctgat ggcaccaact ggaaaatcga caacaaagtg gtccgcgtac cttatgttgg
    10201 cgtagataaa gacaacctgg ctgaattcag caagaaataa gactgattgg gcgcagtcgt
    10261 ttactgcgcc cttattgtat ggccaacaag gtaaaattat atggtcagct caacgactcc
    10321 gtcttccggg gaatacttgt tggaaatgag cggtatcaac aagtcttttc ctggtgttaa
    10381 ggcacttgat aacgttaatt taaaagtccg accacattct atccatgcat taatggggga
    10441 aaacggtgca ggaaaatcga cattattaaa atgcctgttt ggtatttatc aaaaagactc
    10501 cggcaccatt ttattccagg gtaaagagat cgatttccat tccgccaaag aagcactgga
    10561 aaatggtatt tcgatggtac accaggagtt aaacctggta ttacaacgtt cggtgatgga
    10621 caacatgtgg ctggggcgat atcccaccaa aggcatgttt gtcgatcagg acaaaatgta
    10681 ccgcgaaacc aaagcgattt ttgatgaact ggatattgat atcgatccgc gtgcacgcgt
    10741 cggcacatta tccgtttcgc aaatgcagat gatcgaaatt gccaaagcgt tttcctataa
    10801 cgcgaaaatt gtgattatgg atgaaccgac ttcttcgtta accgaaaaag aggtcaatca
    10861 tctgttcact attattcgta aattaaaaga gcgcggctgc ggtattgttt atatctcgca
    10921 taaaatggaa gaaatcttcc agttatgtga tgaagttacc gtattgcgcg acggtcagtg
    10981 gatcgctacc gaaccgctgg cagggctgac gatggacaag atcatcgcca tgatggttgg
    11041 gcgttctctt aaccagcgtt tccctgacaa agaaaacaag ccgggcgaag tgattctgga
    11101 agtgcgtaac ctgacgtcgc tgcgccagcc gtcgattcgc gatgtctcgt ttgatctgca
    11161 taaaggggag attctcggta tagccggtct ggtgggagcg aaacgtaccg atattgttga
    11221 gacgttattt ggtattcgcg agaaatcggc tggcaccatt acgttgcacg gcaaaaagat
    11281 caataaccat aatgccaacg aagccataaa ccacggattt gcactggtaa ctgaagagcg
    11341 ccgctcaacg ggaatttatg cctatctgga tattggcttt aactcgttaa tttccaatat
    11401 ccgcaactat aaaaataaag ttggtttact ggataactcg cggatgaaaa gcgataccca
    11461 gtgggtgatt gattcgatgc gggtaaaaac accggggcat cggacgcaaa ttggttcgct
    11521 ctccggtggt aatcagcaaa aagtgattat tggtcgctgg ctgttaacgc aaccagaaat
    11581 attaatgctc gatgaaccga cgcgcggtat tgatgttggg gctaagtttg agatttatca
    11641 gttaattgcc gaactggcga agaaaggcaa ggggattatt attatctcct ccgaaatgcc
    11701 agagttgtta gggataacag accgtattct ggttatgagc aatggtctcg tttccggaat
    11761 tgtcgataca aaaacaacaa cgcaaagcga aattctgcgt cttgcgtctt tgcaccttta
    11821 agattagggg cttcccatga gtgcattaaa taagaaaagt tttcttactt acctgaaaga
    11881 gggcggtatt tacgtcgttc ttttagtttt gctggcgatt attattttcc aggacccaac
    11941 atttttaagt ctgttgaact taagtaatat tctcacccag tcatcggtgc gtattattat
    12001 cgcgcttggt gtggcagggt taattgtcac ccaggggact gacctttccg caggtcgcca
    12061 ggtggggctg gcggcagtag tagcagcgac gttattgcag tccatggata acgccaacaa
    12121 agtgttcccg gaaatggcga cgatgccgat tgcgctggtt attctgattg tctgtgccat
    12181 tggtgcggtg atcggtttga tcaacggctt gattatcgcc tatctcaacg tgacgccgtt
    12241 cattaccacg ctcggtacga tgattatcgt ctacggcatt aactcgctct attacgactt
    12301 tgtcggggcg tcgccaattt ccggttttga cagtggtttc tccacttttg cccaaggctt
    12361 tattgcgctg gggagtttcc gtctctctta catcaccttc tatgcgttga ttgcggtggt
    12421 gtttgtctgg gtgttatgga acaaaacccg cttcggtaag aacatttttg ccattggcgg
    12481 taacccggaa gcggcgaaag tatctggtgt caacgtcggc ctgaacctgc tgatgattta
    12541 cgcgttgtct ggtgtgttct atgccttcgg cgggatgtta gaagccggac gtatcggctc
    12601 agccaccaac aacctcggct ttatgtatga gctggatgct atcgcggcgt gcgtggtagg
    12661 cggcgtatcg ttcagcggcg gtgtggggac ggtaattggt gtggtgaccg gggtaattat
    12721 ttttaccgtc attaactatg gcctgacgta tatcggcgta aacccgtact ggcagtacat
    12781 catcaaaggg gcgattatta tcttcgccgt ggcgctggat tcactgaaat acgcgcgtaa
    12841 gaagtgatgt gaatgtagtt cgcatccggt cagaaatgca cataacatca tgagttgtat
    12901 ggttttgtat gccggataag gcgtttacgc cgcatccggc gacaggaacc aaatgccgga
    12961 tgcaacgctg gcgcgtttta tccggcctac aatgagcaca acatactgat ttatattgtt
    13021 ttgtatgccg ggtaaggcgt tcacgccgca tccggcgaca ggaactaaat gccggatgcg
    13081 tcgctggttt tacaacgtta ccgggtgttc tttctcgcct ttcttaaact tcacttcccc
    13141 aagatcaata caacctaccg ggcagacatg accacaaagc agacaaccca cgcatttctc
    13201 ggtattacaa tgcggtgtgc gggttttctc gctccattcc atcgcctggt gaccgccgtc
    13261 gtaacaggaa atataacagc gtccacagcc aacacattta tcaagattga tatggggata
    13321 gacaatataa ctgcggtcta aatcttccgc cgggacaata ttgtgattcg ccagacctac
    13381 catctcctgc aacgaatcaa acccttgatc ggcgagataa tgcgacaacc cgcttgccat
    13441 atcttccact atccgatacc cgtactgcat aatgccggtg gtcacctgca acgtcgctgc
    13501 accgagcaat aaaaactccg ccgcatcttc ccaggtttca atgccgccga taccgctgat
    13561 cgggaaatcg cgcagttctg gatgggtgcg catttgctga ataaagcgta gggcgatcgg
    13621 ttttaccgct ttaccggaat atccggaaat actcgattta ccgttaacga tcggcatacc
    13681 aattttctga ttaagatcga tattggtgat ggatttaacg gtgttaatcg ccgcaatgcc
    13741 atctgcgccg ccgcgcttcg ccgccagcgc cacttcgcac atatcaccaa tattcggcgt
    13801 catcttcgcc agcattggca gcgtggaacc acgttttact gcccgacaat atttttctac
    13861 cagctccggg ctttgcccga catcgctacc catcgcatga gaagtcattt gcggacagga
    13921 gaagttacac tcgatcatat ccgcgccagc ttcttgcacc agacgcgcca gctcttccca
    13981 ttgctgctca ttttcgccca tgattgaagc gatcaatact ttgtccggat aatcttcttt
    14041 cagccgacgc agggcggcca gattctcttc caacggatgt tcagcaatct gctccatatt
    14101 tttgaagccg ataaaaccgg tatcttcttt caccagatga tcaaaacgcg gcgagacttc
    14161 gttggcgata aaaaaaccga tcgttttaaa caccacgccg ccccaacctg tgtcgtaggc
    14221 tttggcacac atctcatagc agttgcctac cggcgaagaa gaaaggcaga acgggttggg
    14281 aaacttcacg ccgcaaaaag taatcgaaag atctttcgtt aacatgagca agctccctct
    14341 aaatagtgat gaatcgcccc ggcggcttct ttcccggttt tcactgcata gaccacggtt
    14401 ttgtcaccct caacaatatc gccagcagca aagacttgcg ggtcgcgggt ctggtaatgt
    14461 tgcgtttcga tggtgttacg ctgcggctct aactccgcaa aggcatccag tctggcatgc
    14521 tgaccgacgg cgagaataat tttatctgcc gccatcgtta gttcacccgg taaacgaaca
    14581 tgcttaaagg tgactttatt gccttccacg gctactggtg taaatccatc gatgatcgaa
    14641 acgcccagct ctctggcgct ggtaaactct ttttcgcttg ccggaaactc atctaactct
    14701 tcacgcgcta cgcaggttac cgcctgacag ccgagaactt tcagcgtgct ggctacgtcc
    14761 atcgcgacat caccgccgcc gataattaat gcgctttgtg gaatgctgat atcgccttgc
    14821 gcctgtcgtg cacgttgcag gaagtcgacg gcaatctcaa cgtcgctatg ctcaaacagc
    14881 gataacccgg aaccgctcga taaccctacg gtgaccagta ccgcgcggtt ttccgctttc
    14941 agctgctcca gggtaagtgt tttaccgatt tcgttattgc acttaatggt cacgcccatt
    15001 ttttctatac gggcgatttc tgcatccagc accgattgcg gtaaccggaa ttgcggaatg
    15061 ccgttacgca accagccacc gggttgtgct tctttctcat agatcgtgac gtcataaccc
    15121 tggtttgtca gcgtcacact ggcctgcaat ccggcaggac ctgcgccaat aatggcgact
    15181 ttgccgagcg ttttagtacc gggctgataa atttccatcc cagtttgttg ttcaaaatca
    15241 gtaacaaaac gctgtaaacg accgatatca atgggagcat caacaccggc acgtgtacaa
    15301 ccgctttggc ataatttctc cgtcgggcac actctggcac aaacggcacc gagggcatta
    15361 ttttcgcgaa ttgtctcggc agcgccttta aaattacgaa agtagattga gcgaataaat
    15421 ttccccggat cggtctgtgc cgggcaagcc tgactacagg gagcgtcatg acataataaa
    15481 cagcgagagg cttctttaat cgccagtaaa ggcgtaaaag ccggagtgag ttcatccaga
    15541 taattttgtt gcggcatgtg atcttcctta atgctatgag taattaaggc gaacgctaaa
    15601 taaatattgc gtgctgatat taaggctatg cgcgtgggga aatttaaact gtgaaaggat
    15661 tcacaaatag ctgcggttca gaatgttata tcc
//
LOCUS       DADSYM010000116        15670 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2415
            SAMN08039579-rid11723173.denovo.117, whole genome shotgun sequence.
ACCESSION   DADSYM010000116 DADSYM010000000
VERSION     DADSYM010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039579
            Sequence Read Archive: SRR6362533
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15670)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15670)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980162.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 03:39:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,484
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 4,347
            CDSs (with protein)               :: 4,347
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 43 of 85
            Pseudo Genes (incomplete)         :: 37 of 85
            Pseudo Genes (internal stop)      :: 19 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15670
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039579-rid11723173.denovo.117"
                     /strain="LGE2415"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8302 N 95.9628 W"
                     /collection_date="2015-08-24"
     gene            279..845
                     /gene="yqaB"
                     /locus_tag="J1Y83_003214"
     CDS             279..845
                     /gene="yqaB"
                     /locus_tag="J1Y83_003214"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-1-phosphate/6-phosphogluconate
                     phosphatase"
                     /protein_id="HBA7631335.1"
                     /translation="MYERYAGLIFDMDGTILDTEPTHRKAWREVLGHYGLQYDVQAMI
                     ALNGSPTWRIAQAIIELNQADLDPHALAREKTEAVRSMLLDSVEPLPLVEVVKSWHGR
                     RPMAVGTGSESAIAEALLAHLGLRRYFDAVVAADHVKHHKPAPDTFLLCAQRMGVQPT
                     QCVVFEDADFGIQAARAAGMDAVDVRLL"
     gene            842..1270
                     /locus_tag="J1Y83_003215"
     CDS             842..1270
                     /locus_tag="J1Y83_003215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HBA7631336.1"
                     /translation="MSEALSLFSLFASSFLSATLLPGNSEVVLVAMLLSGVSHPWVLV
                     LTATMGNSLGGLTNVILGRFFPLRKTSRWQEKATGWLKRYGAVTLLLSWMPVVGDLLC
                     LLAGWMRISWGPVIFFLCLGKALRYVAVAAATVQGMMWWH"
     gene            1343..2899
                     /gene="gshA"
                     /locus_tag="J1Y83_003216"
     CDS             1343..2899
                     /gene="gshA"
                     /locus_tag="J1Y83_003216"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HBA7631337.1"
                     /translation="MIPDVSQALAWLEKHPQALKGIQRGLERETLRVNADGTLATTGH
                     PEALGSALTHKWITTDFAEALLEFITPVDGDIEHMLTFMRDLHRYTARNMGDERMWPL
                     SMPCYIAEGQDIELAQYGTSNTGRFKTLYREGLKNRYGALMQTISGVHYNFSLPMAFW
                     QAKCGDISGADAKEKISAGYFRVIRNYYRFGWVIPYLFGASPAICSSFLQGKPTSLPF
                     EKTECGMYYLPYATSLRLSDLGYTNKSQSNLGITFNDLYEYVAGLKQAIKTPSEEYAK
                     IGIEKDGKRLQINSNVLQIENELYAPIRPKRVTRSGESPSDALLRGGIEYIEVRSLDI
                     NPFSPIGVDEQQVRFLDLFMVWCALADAPEMSSSELACTRVNWNRVILEGRKPGLTLG
                     IGCETAQFPLPQVGKDLFRDLKRVAQTLDSINGGEAYQKVCDELVACFDNPDLTFSAR
                     ILRSMIDTGIGGTGKAFAEAYRNLLREEPLEILREEDFVAEREASERRQQEMEAADTE
                     PFAVWLEKHA"
     gene            3049..3564
                     /gene="luxS"
                     /locus_tag="J1Y83_003217"
     CDS             3049..3564
                     /gene="luxS"
                     /locus_tag="J1Y83_003217"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008458247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HBA7631338.1"
                     /translation="MPLLDSFTVDHTRMEAPAVRVAKTMNTPHGDAITVFDLRFCVPN
                     KEVMPERGIHTLEHLFAGFMRNHLNGNGVEIIDISPMGCRTGFYMSLIGTPDEQRVAD
                     AWKAAMEDVLKVQDQNQIPELNVYQCGTYQMHSLQEAQDIARNILERDVRINSNEELA
                     LPKEKLQELHI"
     gene            3760..4218
                     /gene="tnpA"
                     /locus_tag="J1Y83_003218"
     CDS             3760..4218
                     /gene="tnpA"
                     /locus_tag="J1Y83_003218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HBA7631339.1"
                     /translation="MGNEKSLAHTRWNCKYHIVFAPKYRRQVFYREKRRAIGCILRKL
                     CEWKSVRILEAECCADHIHMLVEIPPKMSVSGFMGYLKGKSSLMPYEQFGDLKFKYRN
                     REFWCRGYYVDTVGKNTAKIQDYIKHQLEEDKMGEQLSIPYPGSPFTGRK"
     gene            complement(4327..5439)
                     /locus_tag="J1Y83_003219"
     CDS             complement(4327..5439)
                     /locus_tag="J1Y83_003219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001605992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBA7631340.1"
                     /translation="MIWQLTDDKRWSALRQRFSWVEEMHHTPQDPEHHGEGDVGVHTE
                     MVLNALITLPEFQQLPAQQQEVLWAAALLHDVEKRSTTVQENGRIQSPGHARRGELTA
                     RQILWRDIPTPFVLREQIVALVRLHGLPLWLLERPEPERLLLTAAMRIDTRLLALLAR
                     ADLLGRQSPDQQSMLERIDLFELFCHEQQCWGKMRPFVSDSARWHYLTHEQSSPDFVP
                     WEAEPFEVILLCGLPGMGKDRYINEQCQGMDVISLDDMRRRINASPDDKTATGRIVQQ
                     AKEEARVFLRQKKPFIWNATNITRQLRSQLISLFTAYGARVKIVYLEVPWAQWKQQNA
                     RREYAVPEAVMMRMASRLEVPQLDEAHSVEYRVIER"
     gene            complement(5436..6143)
                     /locus_tag="J1Y83_003220"
     CDS             complement(5436..6143)
                     /locus_tag="J1Y83_003220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001613653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA ligase family protein"
                     /protein_id="HBA7631341.1"
                     /translation="MNTQRKYGRTWHYPFSPGTTSDDRINTDYWRDLQAITQLVHTEK
                     LDGENNCLNRYGVFARSHAAPTQSAWTYKIRQRWQLLKNDLGDLELFGENLYAVHSIE
                     YRALEQDFYLFAVRCQDMWLSWEEVQFYAALFDFPCVPEISGPQPGNDEKSWQRDFLA
                     LTNARGTFDPWDTQTCQPCTLEGIVSRNHDAFSVADFSHNVFKYVRKNHVKTTVHWKR
                     HWQRARMAHEFVYGEQS"
     gene            complement(6401..7939)
                     /gene="emrB"
                     /locus_tag="J1Y83_003221"
     CDS             complement(6401..7939)
                     /gene="emrB"
                     /locus_tag="J1Y83_003221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter permease
                     subunit EmrB"
                     /protein_id="HBA7631342.1"
                     /translation="MQQQKPLEGAQLVIMTIALSLATFMQVLDSTIANVAIPTIAGNL
                     GSSLSQGTWVITSFGVANAISIPLTGWLAKRVGEVKLFLWSTIAFAIASWACGVSSSL
                     NMLIFFRVIQGIVAGPLIPLSQSLLLNNYPPAKRSIALALWSMTVIVAPICGPILGGY
                     ISDNYHWGWIFFINVPIGVAVVLMTLQTLRGRETRTERRRIDAVGLALLVIGIGSLQI
                     MLDRGKELDWFSSQEIIILTVVAVVAICFLIVWELTDDNPIVDLSLFKSRNFTIGCLC
                     ISLAYMLYFGAIVLLPQLLQEVYGYTATWAGLASAPVGIIPVILSPIIGRFAHKLDMR
                     RLVTFSFIMYAVCFYWRAYTFEPGMDFGASAWPQFIQGFAVACFFMPLTTITLSGLPP
                     ERLAAASSLSNFTRTLAGSIGTSITTTMWTNRESLHHAQLTESVNPFNPNAQAMYSQL
                     EGLGMTQQQASGWIAQQITNQGLIISANEIFWMSAGIFLVLLGLVWFAKPPFGAGGGG
                     GGAH"
     gene            complement(7956..9128)
                     /gene="emrA"
                     /locus_tag="J1Y83_003222"
     CDS             complement(7956..9128)
                     /gene="emrA"
                     /locus_tag="J1Y83_003222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter periplasmic
                     adaptor subunit EmrA"
                     /protein_id="HBA7631343.1"
                     /translation="MSANAETQTPQQPVKKSGKRKRLLLLLTLLFIIIAVAIGIYWFL
                     VLRHFEETDDAYVAGNQIQIMSQVSGSVTKVWADNTDFVKEGDVLVTLDPTDARQAFE
                     KAKTALASSVRQTHQLMINSKQLQANIEVQKIALAKAQSDYNRRVPLGNANLIGREEL
                     QHARDAVTSAQAQLDVAIQQYNANQAMILGTKLEDQPAVQQAATEVRNAWLALERTRI
                     VSPMTGYVSRRAVQPGAQISPTTPLMAVVPATNMWVDANFKETQIANMRIGQPVTITT
                     DIYGDDVKYTGKVVGLDMGTGSAFSLLPAQNATGNWIKVVQRLPVRIELDQKQLEQYP
                     LRIGLSTLVSVNTTNRDGQVLANKVRSTPVAVSTAREISLAPVNKLIDDIVKANAG"
     gene            complement(9255..9785)
                     /gene="emrR"
                     /locus_tag="J1Y83_003223"
     CDS             complement(9255..9785)
                     /gene="emrR"
                     /locus_tag="J1Y83_003223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter EmrAB
                     transcriptional repressor EmrR"
                     /protein_id="HBA7631344.1"
                     /translation="MDSSFTPIEQMLKFRASRHEDFPYQEILLTRLCMHMQSKLLENR
                     NKMLKAQGINETLFMALITLESQENHSIQPSELSCALGSSRTNATRIADELEKRGWIE
                     RRESDNDRRCLHLQLTEKGHEFLREVLPPQHNCLHQLWSALSTTEKDQLEQITRKLLS
                     RLDQMEQDGVVLEAMS"
     gene            9756..9857
                     /locus_tag="J1Y83_003224"
     CDS             9756..9857
                     /locus_tag="J1Y83_003224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005228386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA7631345.1"
                     /translation="MFNGRKRTIHLGMTSLISADIVTTVTNKVNVLF"
     gene            complement(9876..10211)
                     /gene="ygaH"
                     /locus_tag="J1Y83_003225"
     CDS             complement(9876..10211)
                     /gene="ygaH"
                     /locus_tag="J1Y83_003225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-valine transporter subunit YgaH"
                     /protein_id="HBA7631346.1"
                     /translation="MSYEVLLLGLLVGAANYCFRYLPLRLRVGNARPTKRGAIGILLD
                     TIGIASICALLVVSTAPEVMHDTRRFVPTLVGFAVLGASFYKTRSIIIPTLLSALAYG
                     LAWKVMAII"
     gene            complement(10201..10938)
                     /locus_tag="J1Y83_003226"
     CDS             complement(10201..10938)
                     /locus_tag="J1Y83_003226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HBA7631347.1"
                     /translation="MESPTPQPAPGSATFMEGCKDSLPIVISYIPVAFAFGLNATRLG
                     FSPLESVFFSCIIYAGASQFVITAMLAAGSSLWVAALTVMAMDVRHVLYGPSLRSRII
                     QRLQKSKTALWAFGLTDEVFAAATAKLVRNNRRWSENWMIGIAFSSWSSWVFGTVIGA
                     FSGSGLLQGYPAVEAALGFMLPALFMSFLLASFQRKQSLCVTAALVGALAGVTLFSIP
                     VAILAGIVCGCLTALIQAFWQGAPDEL"
     gene            complement(11062..12246)
                     /locus_tag="J1Y83_003227"
     CDS             complement(11062..12246)
                     /locus_tag="J1Y83_003227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBA7631348.1"
                     /translation="MTKPNHELSPALIVLMSIATGLAVASNYYAQPLLDTIARNFSLS
                     ASSAGFIVTAAQLGYAAGLLFLVPLGDMFERRRLIVSMTLLAAGGMLITASSQSLAMM
                     ILGTALTGLFSVVAQILVPLAATLASPDKRGKVVGTIMSGLLLGILLARTVAGLLANL
                     GGWRTVFWVASMLMALMALALWRGLPQMKSETHLNYPQLLGSVFSMFISNKILRTRAL
                     LGCLTFANFSILWTSMAFLLAAPPFNYSDGVIGLFGLAGAAGALGARPAGGFADKGKS
                     HHTTTFGLLLLLLSWLAIWFGHTSVLALIIGILVLDLTVQGVHITNQTVIYRIHPDAR
                     NRLTAGYMTSYFIGGAAGSLISASAWQHGGWAGVCLAGATIALVNLLVWWRGFHRQEA
                     AN"
     gene            complement(12438..13430)
                     /gene="proX"
                     /locus_tag="J1Y83_003228"
     CDS             complement(12438..13430)
                     /gene="proX"
                     /locus_tag="J1Y83_003228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     substrate-binding protein ProX"
                     /protein_id="HBA7631349.1"
                     /translation="MRHSVLFATAFATLISTQTFAADLPGKGITVNPVQSTITEETFQ
                     TLLVSRALEKLGYTVNKPSEVDYNVGYTSLASGDATFTAVNWTPLHDNMYEAAGGDKK
                     FYREGVFVNGAAQGYLIDKKTADQYKITNIAQLKDPKIAKLFDTNGDGKADLTGCNPG
                     WGCEGAINHQLAAYGLTNTVTHNQGNYAAMMADTISRYKEGKPVFYYTWTPYWVSNEL
                     KPGKDVVWLQVPFSALPGDKNADTKLPNGANYGFPVSTMHIVANKAWAEKNPAAAKLF
                     AIMQLPVADINAQNAIMHDGKASEGDIQGHVDGWIKAHQQQFDGWVNEALAAQK"
     gene            complement(13488..14552)
                     /gene="proW"
                     /locus_tag="J1Y83_003229"
     CDS             complement(13488..14552)
                     /gene="proW"
                     /locus_tag="J1Y83_003229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     permease ProW"
                     /protein_id="HBA7631350.1"
                     /translation="MADQNNPWDTTPAADSAAQSADAWGTPATAPTDGGGADWLTSTP
                     APNVEHFNILDPFHKTLIPLDSWVTEGIDWVVTHFRPVFQGVRVPVDYILNGFQQLLL
                     GMPAPVAIIVFALIAWQISGVGMGVATLVSLIAIGAIGAWSQAMVTLALVLTALLFCI
                     VIGLPLGIWLARSPRAAKIIRPLLDAMQTTPAFVYLVPIVMLFGIGNVPGVVVTIIFA
                     LPPIIRLTILGINQVPADLIEASRSFGASPSQMLFKVQLPLAMPTIMAGVNQTLMLAL
                     SMVVIASMIAVGGLGQMVLRGIGRLDMGLATVGGVGIVILAIILDRLTQAVGRDSRSR
                     GNRRWYTTGPVGLLTRPFIK"
     gene            complement(14545..>15670)
                     /gene="proV"
                     /locus_tag="J1Y83_003230"
     CDS             complement(14545..>15670)
                     /gene="proV"
                     /locus_tag="J1Y83_003230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein ProV"
                     /protein_id="HBA7631351.1"
                     /translation="QGLSKEQILEKTGLSLGVKDASLAIEEGEIFVIMGLSGSGKSTM
                     VRLLNRLIEPTRGQVLIDGMDIAKISDAELREVRRKKIAMVFQSFALMPHMTVLDNTA
                     FGMELAGINAEERREKALDALRQVGLENYAHSYPDELSGGMRQRVGLARALAINPDIL
                     LMDEAFSALDPLIRTEMQDELVKLQAKHQRTIVFISHDLDEAMRIGDRIAIMQNGEVV
                     QVGTPDEILNNPANDYVRTFFRGVDISQVFSAKDIARRTPNGLIRKTPGFGPRSALKL
                     LQDEDREYGYVIERGNKYVGAVSIDSLKAALTQQQGLDAALIDAPLAVDAQTPLSELL
                     SHVGQAPCAVPVVDEDQQYVGIISKGMLLRALDREGVNNG"
BASE COUNT         3896 a         3996 c         4165 g         3613 t
ORIGIN      
        1 ttctccgtaa ccttcagcaa tgaaggtatc gaagaggtag cggtattaac cgcaggcacc
       61 agggaggata acgttgcgcc agcagcggcc cgaagggcga acgccagtga gtcatcctcc
      121 cggatgcacc atcttctcca aaaaatttcc ttcaaaaata tctcatttct aaataactac
      181 tttatccatt tcctgaattt tcttctcctt ctccatcagc gacaaaatca agcaattacg
      241 ctaaagtagc gtcatatttt tctacttgcg agaagaccat gtacgagcgt tatgcaggtt
      301 taatttttga tatggatggc accatcctgg ataccgagcc tacgcaccgt aaagcgtggc
      361 gcgaagtatt agggcactac ggtcttcagt acgatgttca ggcgatgatt gcgcttaatg
      421 gctcgcccac ctggcgtatt gctcaggcga ttattgagct gaatcaggcc gatctcgacc
      481 cgcatgcgtt agcgcgtgaa aaaacagaag cagtaagaag tatgctgctg gatagtgtcg
      541 agccgcttcc tcttgttgaa gtggtgaaaa gctggcatgg tcgtcgccca atggctgtag
      601 gaacggggag tgaaagcgcc atcgctgagg cattgctggc acacctggga ttacgccgtt
      661 attttgacgc cgtcgtcgct gccgatcacg tcaaacacca taaacccgcg ccagacacat
      721 ttttgttgtg cgcgcagcgt atgggcgtgc aaccgacgca gtgtgtggtc tttgaagatg
      781 ccgatttcgg tattcaggcg gcccgtgcag caggcatgga cgccgtggat gttcgcttgc
      841 tgtgagtgaa gcgttatcgc ttttctcgtt gtttgccagt agttttctca gcgctacact
      901 attacccggc aactccgaag tcgtgctggt ggcaatgttg ctttccggag tgagtcatcc
      961 ctgggtttta gttttaacag caacaatggg taatagcctt ggagggttaa ctaacgttat
     1021 ccttgggcgt ttctttccat tgcgtaaaac atcgcgctgg caagagaaag ctaccggctg
     1081 gctgaaacgc tatggtgcag tcacgctatt attaagctgg atgcccgtgg ttggcgattt
     1141 actgtgtctg ttagcgggat ggatgcgcat ctcgtgggga ccggtaatct tttttttgtg
     1201 ccttggtaaa gcgttacgct atgttgcagt tgcagcagct accgttcagg gcatgatgtg
     1261 gtggcactaa ttgtaggcct gcacatatgg tcaccattac agttatgcta attaaaacga
     1321 ttttgacagg cgggaggtca atttgatccc ggacgtatca caggcgctgg cctggctgga
     1381 aaaacatcct caggcgttaa aggggataca gcgtgggctg gagcgcgaaa ctttgcgtgt
     1441 taatgctgat ggcacactgg caacaacagg tcatcctgaa gcattaggtt ctgcactgac
     1501 ccataaatgg attactaccg attttgcgga agcattgctg gaatttatta caccagtgga
     1561 tggtgatatt gaacatatgc tgacctttat gcgcgatctg catcgctata cggcgcgcaa
     1621 tatgggcgat gagcggatgt ggccgttaag tatgccatgc tatatcgcag aaggtcagga
     1681 catcgaactg gcacagtacg gcacttctaa caccggtcgc tttaaaacgc tgtaccgtga
     1741 agggctgaaa aatcgctacg gcgcgctgat gcaaaccatt tccggcgtgc actataattt
     1801 ctctttgcca atggcattct ggcaagcgaa gtgcggtgat atctcgggcg ctgatgccaa
     1861 agagaaaatt tctgcgggct atttccgcgt tatccgcaat tactatcgtt tcggttgggt
     1921 cattccttat ctgtttggtg cgtctccagc gatttgttct tctttcttgc aaggaaaacc
     1981 aacgtcgctg ccgtttgaga aaaccgagtg cggtatgtat tacctgccgt atgcgacctc
     2041 tcttcgtttg agcgatctcg gctataccaa taaatcgcaa agcaatcttg gtatcacctt
     2101 caatgatctc tacgagtacg tggcgggcct taaacaggca atcaaaacgc catcggaaga
     2161 gtacgcgaag attggtattg agaaagacgg taagcggctg caaatcaaca gcaacgtgct
     2221 gcagattgaa aacgaactgt acgcgccgat tcgtccaaaa cgcgttaccc gcagcggcga
     2281 gtcgccttct gatgcgctgt tacgtggcgg cattgaatat attgaagtcc gctcgctgga
     2341 catcaacccg ttctcgccga ttggtgtaga tgaacaacag gtgcgattcc tcgacctgtt
     2401 tatggtctgg tgtgcgctgg ctgatgcacc ggaaatgagc agtagcgaac ttgcctgtac
     2461 acgcgttaac tggaaccggg tgatcctcga aggtcgcaaa ccgggcctga cgctgggtat
     2521 cggctgcgaa accgcgcagt tcccgttacc gcaggtgggt aaagatctgt tccgcgatct
     2581 gaaacgcgtc gcgcaaacgc tggatagcat taacggcggc gaagcgtatc agaaagtgtg
     2641 tgatgaactg gtcgcctgct tcgataatcc tgatctgact ttctctgccc gtatcttaag
     2701 gtctatgatt gatactggta ttggcggaac aggcaaagcg tttgctgaag cgtaccgtaa
     2761 tctgctgcgt gaagagccgc tggaaattct gcgcgaagag gattttgtag ccgagcgcga
     2821 ggcgtctgaa cgccgtcagc aggaaatgga agccgctgat accgaaccgt ttgcggtgtg
     2881 gctggaaaag cacgcctgac agaaaagaaa aaggccactc gtgagtggcc aaaatttcat
     2941 ctctgaattc agggatgatg ataacaaatg cgcgtctttc atatactcag actcgcctgg
     3001 gaacaaagag ttcagaaaat tttaaaaaaa attaccggag gtggctaaat gccgttgtta
     3061 gatagcttca cagtcgatca tacccggatg gaagcgcctg cagttcgggt ggcgaaaaca
     3121 atgaacaccc cgcatggcga cgcaatcacc gtgttcgatc tgcgcttctg cgtgccgaac
     3181 aaagaagtga tgccagaaag agggatccat accctagagc acctgtttgc tggttttatg
     3241 cgtaaccatc ttaacggtaa tggcgtagag attatcgata tctcgccaat gggctgccgc
     3301 accggttttt atatgagtct gattggtacg ccagatgagc agcgtgtcgc tgatgcctgg
     3361 aaagcggcaa tggaagacgt gctgaaagtg caggatcaga atcagatccc ggaactgaac
     3421 gtctaccagt gtggcactta ccagatgcac tcgttgcagg aagcgcagga tattgcgcgt
     3481 aacattctgg aacgtgacgt gcgcatcaac agcaacgaag aactggcgct gccgaaagag
     3541 aagttgcagg aactgcacat ttagtcagtg gaacaaaaga agccgggcag gtgacaccac
     3601 cgcccgattt tttgttgtgc acagaaaacc cccagctagg ctgggggttc cggaaagctt
     3661 tcagctttga gccagttatt aaaacccctt ttgatttgtt aaaacacctt gcggtctggc
     3721 aactgcaagt gtcaaacaag aaatcaaaag ggggtcccaa tggggaacga aaagagctta
     3781 gcgcacaccc gatggaactg taaatatcac atagtatttg cgccaaaata ccgaagacag
     3841 gtgttctaca gagagaagcg tagagcaata ggctgtattt tgagaaagct gtgtgagtgg
     3901 aaaagtgtac ggattctgga agctgaatgc tgtgcagatc atatccatat gcttgtggag
     3961 atcccgccca aaatgagcgt atcaggcttt atgggatatc tgaaagggaa aagcagtctg
     4021 atgccttacg agcagtttgg tgatttgaaa ttcaaataca ggaacaggga gttctggtgc
     4081 agagggtatt acgtcgatac ggtgggtaag aacacggcga agatacagga ttacataaag
     4141 caccagcttg aagaggataa aatgggagag cagttatcga ttccctatcc gggcagcccg
     4201 tttacgggcc gtaagtaacg aagttggatg caaatgtcag atcgtgtgcg cccgttaggg
     4261 cgcggctggt aagagagcct taaaggcgca tttgaaaaac ctccggctat gccggaggat
     4321 atttatttac ctttcaatca cccgatactc cacgctatgt gcttcatcaa gttgaggaac
     4381 ttccagcctt gaggccatcc gcatcatgac cgcttcagga actgcatact cgcgtctggc
     4441 attttgctgc ttccattgcg cccatggcac ttccagataa acgattttta ctcgtgcgcc
     4501 gtaagccgta aacagactga tgagctggct gcgtagctgg cgggtaatgt tcgttgcatt
     4561 ccagatgaac ggctttttct ggcgtaaaaa aacgcgcgcc tcttcttttg cctgttgcac
     4621 aatacgcccg gtagcggttt tgtcgtccgg actggcattg attcgccgcc gcatatcatc
     4681 aaggctgata acgtccattc cctggcactg ttcattgata tagcgatctt tgcccatacc
     4741 cggtaagcca cacagcaaaa tgacctcaaa aggttcagcc tcccagggaa caaaatcagg
     4801 agaactttgc tcatgggtca ggtaatgcca gcgtgcggaa tcagaaacga acgggcgcat
     4861 ttttccccag cattgttgct catggcaaaa cagctcgaac agatcgatgc gttccagcat
     4921 tgattgctga tccgggcttt ggcgaccaag cagatcggca cgagcaagca aagcgagcag
     4981 acgcgtgtcg atgcgcattg cagccgtgag caacaaacgc tccggttccg ggcgttccag
     5041 taaccatagc ggtaacccat gcagacgcac cagtgcaaca atttgctcgc gcagcacgaa
     5101 cggcgttggg atatcgcgcc ataaaatttg tcgggccgtc agttcgcccc gacgagcatg
     5161 accaggagac tgaatacgtc cgttttcctg cacggtagtg ctgcgctttt caacatcatg
     5221 caataacgcc gccgcccata aaacttcctg ctgttgagcg gggagttgct ggaattctgg
     5281 cagagtaatg agggcgttaa gcaccatttc ggtatgtacg ccgacgtcgc cttcaccatg
     5341 atgctcagga tcttgtggcg tgtgatgcat ctcctcaacc cagctaaaac gctgacgtag
     5401 cgccgaccag cgtttatcgt cggtaagttg ccaaatcatg actgttctcc ataaacgaac
     5461 tcatgggcca tccgcgcccg ttgccagtgg cgtttccagt gcacagtggt tttgacatga
     5521 tttttgcgca catacttgaa gacgttatgc gaaaagtcag cgacagaaaa cgcatcatga
     5581 tttcggctga caatgccttc cagcgtacag ggttgacagg tttgcgtatc ccagggatcg
     5641 aatgtgccgc gcgcattggt cagcgccaga aaatcacgtt gccagctttt ttcatcattc
     5701 cccggctgcg ggccggagat ttcgggaacg caggggaaat caaacagagc ggcgtaaaac
     5761 tgcacctctt cccagctcag ccacatatcc tggcagcgaa cggcaaacag gtaaaagtcc
     5821 tgttccagcg cgcggtattc aatggaatga acggcgtaga gattttcacc aaacagttcc
     5881 agatcgccga gatcgttttt cagcagttgc caacgctggc gaattttata agtccatgcc
     5941 gactgcgtag gcgcagcatg ggaacgggca aacacgccgt agcggttaag gcagttattt
     6001 tcgccgtcga gtttttctgt gtgcaccagt tgcgtgatgg cctgtaaatc ccgccagtaa
     6061 tcggtgttaa tgcggtcatc gctggtggtg ccaggggaga aaggataatg ccaggttctc
     6121 ccgtatttcc gttgggtatt cataataatc cattattttg tgtgtaataa atgtacgccc
     6181 gcgagggtgc gagttatggg gttaaaagaa aaatgtgagt atcacccgcg aaagcgaggg
     6241 cgaaggaaaa agagaattaa caacctgagc gaatgctcag aaccggttaa tggtgaatgg
     6301 aagagtttgg ggcggcgaaa agatacaccg aatgagtgga ttaaacaacc gtttacagta
     6361 tgagcaaata aaaagggagc cgcagctccc tttgacaacc ttagtgcgca ccgcctccgc
     6421 cgccacctgc gccaaagggc ggtttagcaa accacaccag ccccagcaag acgaggaata
     6481 tcccggctga catccagaag atctcattgg cggaaataat cagcccctga ttggtgatct
     6541 gctgggcaat ccagcctgat gcctgctgtt gcgtcatacc aagcccttcc agttgactgt
     6601 acatcgcctg agcattcggg ttgaacgggt ttaccgactc agtcaactgt gcatggtgca
     6661 gtgactcgcg gttggtccac atggtcgtgg ttatcgacgt gccgatagac cccgccagcg
     6721 ttcgcgtaaa gttggagagg ctcgatgccg ccgccagtcg ttccggtggc aaaccggaca
     6781 gcgtaatggt ggtcagcggc ataaagaagc aggccaccgc aaaaccctgg ataaactgtg
     6841 gccaggccga cgcgccaaaa tccatacccg gttcaaaggt ataggcacgc cagtagaagc
     6901 agacggcata cataataaag ctgaaggtta ccagtcggcg catatccagt ttatgcgcaa
     6961 agcggccgat aatcggcgac aggatcaccg gaataatccc taccggcgca gaggccagcc
     7021 ctgcccacgt cgccgtgtaa ccgtagacct cctgcaacaa ctgcggcagc agaacaatcg
     7081 cgccgaagta gagcatatac gcgaggctga tacacaagca gccgatggtg aagttgcgcg
     7141 acttaaacaa cgacagatcg actatcgggt tatcgtcggt cagctcccag acaatcagga
     7201 agcagatagc caccaccgcc accacggtga ggatgataat ttcctgtgat gagaaccagt
     7261 ccagctcttt accgcggtcg agcataatct gcaggctgcc gataccaata accagcagtg
     7321 ccagccccac ggcatcaatc cgccgccgtt cggtgcgggt ttcacgtccg cgcagagttt
     7381 gcagggtcat caacaccacc gccacgccaa tcggcacgtt gatgaagaat atccagcccc
     7441 agtggtaatt atcactgata taaccgccga ggatcgggcc gcaaattggc gcaacaatca
     7501 ccgtcatcga ccacaacgcc agcgcgatcg agcgtttggc tggcgggtag ttattcagca
     7561 atagactttg tgaaagcggg atcagcggcc cggcgacaat cccctgaatc acgcggaaga
     7621 agatcagcat attcaggctg ctggagacac cacacgccca cgacgcaata gcaaaggcga
     7681 tggttgacca gaggaacaat ttcacttccc cgacgcgctt tgccagccag ccggtaagcg
     7741 ggatcgagat ggcattcgcc accccgaaag aagtgatcac ccacgttccc tgactgagtg
     7801 atgagcccag attcccggcg atagtgggga tcgccacgtt agcaatggtg gagtccagca
     7861 cctgcatgaa tgtcgccagt gacagcgcaa tcgtcataat gaccagttgt gcgccttcca
     7921 gcggtttttg ctgttgcatc acacgcacct ctggattagc cagcgttagc ttttacgata
     7981 tcgtcgatca gtttattgac aggtgccagg ctgatttcac gcgcggtgct taccgcgacc
     8041 ggtgtggaac gtactttatt tgccagtacc tgaccgtcac ggttagtggt attgacactc
     8101 accagcgtgg ataaaccgat acgcagcggg tattgctcca gctgtttctg gtccagttca
     8161 atacgcacag gcagacgctg aacgactttg atccagttac cggtggcgtt ttgcgccgga
     8221 agcagtgaga acgcgctacc tgtgcccata tccagaccaa ccactttacc ggtgtatttc
     8281 acatcatcgc cgtaaatatc ggtggtgatg gtgaccggct gaccgatacg catattggca
     8341 atctgcgtct ctttaaagtt ggcatccacc cacatattgg tggctggaac gaccgccatc
     8401 agcggcgtcg ttgggctaat ttgcgcccca ggctgtaccg cgcggcggga gacataaccg
     8461 gtcatcggac tgacaatacg ggtacgctcc agcgccagcc aggcgttacg tacttcggtg
     8521 gcagcctgtt gtacggcagg ctgatcttcc agtttcgtcc ccagaatcat cgcctgattg
     8581 gcattgtatt gttgaatagc gacgtccagt tgcgcctggg cactggtgac ggcgtcgcgg
     8641 gcgtgttgca gctcttcgcg accaatcagg ttggcattgc ccagcggcac acggcggttg
     8701 tagtcgcttt gtgctttcgc gagggcgatt ttttgcacct caatattcgc ctgcaactgt
     8761 ttgctgttaa tcatcagctg gtgggtttgg cgaacgctgg aagccagtgc agttttggct
     8821 ttttcaaatg cctggcgagc atctgtcggg tcgagagtga ccagcacgtc gccttctttt
     8881 acaaaatcgg tgttatcggc ccagactttc gtcacgctgc cagacacctg agacataatt
     8941 tgaatctgat tccctgccac gtatgcgtca tcggtttctt cgaaatgacg cagtaccaaa
     9001 aaccaataaa tccctatcgc tacggcaata attataaaga gcaaggtgag aaggaggagc
     9061 agacgcttac gtttgccgct cttctttacc ggttgctgcg gggtttgagt ctccgcattt
     9121 gcgctcatat tgttctccac gatcttctta actcatcggc tgagccgacc tgcttgccaa
     9181 aaaggccagc atgttacgat gctggccggt tattttttct tttataaatc tggatttttg
     9241 agcgagatga cgcgttagct catcgcttcg agaaccacac cgtcctgttc catctggtcg
     9301 agacgggaga gcaatttgcg ggtgatttgc tcgagctggt ctttttctgt tgtgctgagc
     9361 gcggaccaga gttgatgcag gcagttatgc tgcggcggta aaacctcgcg caaaaactcg
     9421 tgaccttttt ccgttaattg cagatgcagg cagcggcgat cgttatcgct ttcacgacgt
     9481 tcgatccaac cgcgtttttc cagttcatcg gcaatacgcg tcgcgttggt acgggatgat
     9541 ccaagagcac aactcaattc agaaggctga atactgtggt tttcctgaga ctccagcgta
     9601 atcaacgcca taaacaacgt ctcgttaatc ccctgagcct tcagcatttt attgcggttc
     9661 tccagcagct tgctttgcat gtgcatgcaa agacgagtca gaaggatctc ctgataagga
     9721 aaatcttcgt ggcggctggc gcgaaatttt agcatttgtt caatgggcgt aaacgaacta
     9781 tccatttggg tatgacctca ttaatttcag ccgatatagt aacgacagtg acaaataaag
     9841 taaatgtatt gttttagaaa aatgattctt gtgggttata taatcgccat cactttccag
     9901 gcgagcccgt aggccagcgc actaagcagt gttgggatga taatgctgcg tgttttatag
     9961 aaactggcac ccagtaccgc gaagccgacc agcgtgggca cgaaacggcg tgtatcgtgc
    10021 atcacttctg gtgcggtaga gacaaccagc agagcgcata tcgaggcgat gccaatggtg
    10081 tcgagcaata tgcctatcgc gccacgtttg gttgggcggg cattacccac acgcaggcgc
    10141 agcggcaaat agcggaagca ataattcgcc gcgccaacca gtaacccaag cagcagaacc
    10201 tcatagctca tcgggcgctc cttgccagaa tgcctggatt aacgcagtga ggcagccaca
    10261 gacaatgcct gccagaatgg cgacgggaat agaaaatagc gttacgcctg caagggcacc
    10321 aactaacgct gcggtaacgc aaagagattg tttgcgctgg aaagaggcga gcaggaaact
    10381 cataaagagt gccggaagca taaaacctaa tgctgcttca acggcgggat aaccttgcag
    10441 caagccgctg ccggagaatg cccctattac cgtaccaaat acccacgatg accatgaact
    10501 gaaggcaatg ccgatcatcc agttttcgct ccagcggcga ttattgcgta ccagttttgc
    10561 ggttgcggcg gcaaaaacct catccgtcag gccaaacgcc cacagggcgg ttttcgattt
    10621 ttgcagacgc tgaataatac ggctacgcag tgacgggcca tacaaaacat ggcgaacatc
    10681 cattgccatg acggtcagtg cagcaaccca caaactactc ccggctgcca gcatcgcggt
    10741 aatgacgaac tggctcgcgc ctgcataaat gatgcaggag aaaaaaacgc tttcgagagg
    10801 agagaatccc agacgggtcg cattcagacc gaacgcaaag gccaccggaa tataactaat
    10861 aacaatcggt aaactgtctt tgcatccttc catgaaggtc gccgaaccag gagcaggctg
    10921 tggagtaggg ctttccatag agtgtacgct taacgattgt taatattata aatagactga
    10981 atgaatatct taaccttatc agactgatgg gcttcttaac acccttataa gtgtaaagcc
    11041 acgaaaacgg ttgctgattg cttaatttgc ggcttcctga cgatgaaaac ctcgccacca
    11101 gaccagtaag ttaaccaggg caatcgtcgc gccagccaga cacacgccag cccaaccgcc
    11161 atgttgccag gctgaggctg aaattagcga accggcggca ccgccaataa agtagctggt
    11221 catgtaacct gcggtcaggc gattacgggc atcagggtgt attcgataaa ttaccgtctg
    11281 gttagtgata tgcacgccct gcacggtgag gtccagcacc aggatgccga taatcagcgc
    11341 cagtacggaa gtgtgaccaa accagattgc cagccatgaa agtaacagca gcagcagacc
    11401 gaaagttgtg gtgtggtgcg atttgccctt atcggcaaaa ccgcccgccg gacgagcgcc
    11461 caacgctccg gcagctcccg caagcccaaa cagaccaatt acaccatcgc tgtagttaaa
    11521 aggtggagcg gcaagcaaaa aggccattga ggtccagaga atgctgaagt tggcaaaggt
    11581 caggcagccc agcaacgcgc gggtgcgcag gatcttattg ctgataaaca tgctgaaaac
    11641 ggaacccaac aactgtgggt agttgaggtg ggtttctgat ttcatttgtg gcagaccacg
    11701 ccataatgcc agcgccatca gtgccattaa catcgaagca acccaaaaga cggtgcgcca
    11761 gccgccgaga ttcgccagca atccggcaac ggtccgtgcg agtaagatcc ccaatagcag
    11821 accgctcata atggtgccaa ctactttgcc gcgtttgtcc ggtgaagcca gcgtcgctgc
    11881 cagtggaacc agaatttgtg ccacgactga aaataaaccg gttaatgctg taccgaggat
    11941 catcatcgcc agcgactgac tgctggcagt gattaacatg ccgcctgctg ccagtaaggt
    12001 catcgagaca atcaggcggc ggcgttcaaa catatcaccg aggggaacaa gaaacagcag
    12061 acctgcggca tagcccaact gcgcggcggt aacaataaag cctgccgaac tggcggaaag
    12121 ggaaaagtta cgcgcgatgg tgtcgagcaa tggctgggcg taatagttac tggctaccgc
    12181 cagaccggtg gcgatagaca tcagcacgat cagcgccggg ctaagctcat gattaggttt
    12241 agtcattatt tttgttcatc aggtgatgat taagctggaa atcaataata gcggatgttg
    12301 atgaatatgg gatgaaagat agattgttga aatgtgcggt gtggtttgta ggtatgataa
    12361 ggcgcgccag cgtcgcatca ggcattgtgt acagagcggc ggatgaggca aaaacgcctt
    12421 atccgcccga ataaaaatta cttctgcgct gccagcgctt cattcaccca gccatcgaac
    12481 tgctgctggt gggctttgat ccaaccatca acgtggccct gaatatcgcc ttctgaggct
    12541 ttgccgtcat gcataatggc gttctgggcg ttaatatctg ccactggtaa ctgcataatg
    12601 gcaaacagtt tcgctgctgc cgggtttttc tcggcccagg ctttgttggc aacgatatgc
    12661 atggtactga ccgggaagcc gtaattcgcg ccattcggca gtttggtatc ggcattttta
    12721 tcgcccggca gtgcggagaa cggcacctgc aaccagacca catctttccc cggtttcagt
    12781 tcgttactca cccagtacgg cgtccaggtg tagtaaaaca ccggcttacc ctctttgtaa
    12841 cgactgatgg tgtcagccat catcgctgcg tagttcccct gattatgcgt cacggtattg
    12901 gtcagtccat acgcggcaag ctggtggttg atcgcacctt cgcagcccca gcctgggtta
    12961 cagccggtta aatccgcttt accgtcgccg ttggtatcga acagtttggc gatcttcgga
    13021 tctttaagtt gcgcgatgtt ggtaatttta tactggtcgg cagttttctt atcgatcagg
    13081 taaccctgtg ccgcgccgtt aacaaatact ccttcacgat aaaatttctt atcgccaccg
    13141 gcagcttcgt acatgttgtc atgcagtggc gtccagttca ctgcggtgaa ggttgcatcg
    13201 ccggaagcaa gtgaggtgta gccaacgttg taatccactt cgctgggttt attgacggta
    13261 taacctaatt tctccagcgc acgactgacc agcagtgtct ggaaggtttc ttcggtgatg
    13321 gtgctttgaa ccggattaac agtaatgcct ttgcccggca ggtcggcagc aaaagtttgt
    13381 gtagagataa gcgtggcaaa cgctgtcgca aaaagtacgc tatgtcgcat tgttattcct
    13441 ttttttggca gggtgataac gcccggcgtc accgggcaag tgcagagtta cttaatgaat
    13501 gggcgggtca gcagaccgac agggccagtg gtgtaccagc gacggttgcc acgactgcgt
    13561 gagtcgcgcc caacggcctg cgtcaggcgg tcgagtataa tggcgaggat cacaatcccg
    13621 acgccgccaa cggtggcaag ccccatatcc agacgaccga taccgcgaag taccatctga
    13681 cccagcccgc cgacggcaat catcgaggcg atgaccacca tagaaagggc cagcatcagc
    13741 gtctggttaa cgcccgccat aatggttggc atcgccagcg gtaactgaac tttgaacagc
    13801 atttggctcg ggctggcacc gaatgagcgc gaggcttcaa tcagatccgc cggaacctgg
    13861 ttaattccca gaatcgtcag acggataatc ggcggcagcg caaagatgat cgtcaccacc
    13921 acgcccggca cgttgccgat accaaacagc atgacgattg gcaccagata aacaaacgcg
    13981 ggcgtggtct gcatggcatc aagcagtgga cgaataattt tcgccgctct cggacttctc
    14041 gccagccaga tccccaacgg caaacctatg acgatacaga acagcagggc tgttaacacc
    14101 agcgccaggg taaccattgc ctgtgaccag gcaccgattg cgccgatggc aatcagcgaa
    14161 accagcgtcg ccacgcccat tccgaccccg gaaatctgcc aggcgatgag agcgaaaacg
    14221 ataatcgcca ccggcgcggg catacccagc agcaattgct ggaaaccgtt gaggatgtaa
    14281 tcaaccggaa cgcgcacgcc ctgaaagacg ggacgaaaat gggtaacgac ccagtcgatc
    14341 ccttcagtga cccaactgtc gagcgggatc agcgttttat ggaacggatc gagaatatta
    14401 aaatgctcga cgtttggcgc aggcgtactg gtcagccagt cagcgccacc gccgtcagtc
    14461 ggtgcagtcg ccggtgtacc ccaggcgtct gcggattgtg cagcactgtc cgccgctggc
    14521 gtggtatccc acggattatt ttgatcagcc attatttacc ccctcacgat ctaaagcgcg
    14581 cagtagcatt cctttcgaaa tgatgccgac atactgttgg tcctcgtcga ccacgggcac
    14641 cgcacagggt gcctgtccga catgagagag caattcgcta agaggcgttt gtgcatcgac
    14701 cgctaacggc gcatcaatca gcgccgcatc aagaccttgc tgctgcgtta atgcggcttt
    14761 aagcgaatcg atagacacag cgccgacata cttattaccg cgttcgatga cgtagccata
    14821 ttcgcgatct tcatcctgca ataatttcag tgccgaacgc gggccgaagc cgggggtttt
    14881 acgaattaag ccgttcggtg tacgacgggc aatatctttc gcactgaaca cctggctaat
    14941 atcgacgcca cggaagaagg tacgaacata atcattcgcc ggattattaa gaatttcatc
    15001 cggtgtacca acttgcacca cttcaccatt ttgcataatg gcaattcggt caccaatacg
    15061 catagcttca tcgagatcgt gggaaataaa gacaatggtg cgctgatgtt tcgcctgtaa
    15121 ttttaccagc tcatcctgca tctcggtgcg aattaatgga tcgagtgctg agaaggcttc
    15181 gtccattaat aatatatccg gattaatcgc taacgcgcgg gctaatccca cacgttgacg
    15241 catcccgcca gagagttcat ccgggtagct gtgggcataa ttttccagcc cgacctgacg
    15301 cagtgcatca agggcttttt cccggcgttc ttcggcatta attccggcca gttccatacc
    15361 gaacgcagta ttgtccagca cggtcatatg cggcattaag gcaaaggact ggaagaccat
    15421 cgcaatcttt tttctgcgca cctcacggag ttcggcgtcg gatattttgg caatatccat
    15481 accatcaatc agcacttgcc cgcgggtggg ttcaatcagg cgattgagaa ggcgtaccat
    15541 tgtggattta cccgaaccgg ataatcccat gatgacaaat atctcgcctt cttcaatggc
    15601 cagactggcg tctttaacgc caagcgatag cccagttttt tccagaattt gttcttttga
    15661 aagtccttgt
//
LOCUS       DADSYM010000117        15604 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2415
            SAMN08039579-rid11723173.denovo.118, whole genome shotgun sequence.
ACCESSION   DADSYM010000117 DADSYM010000000
VERSION     DADSYM010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039579
            Sequence Read Archive: SRR6362533
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15604)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15604)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980162.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 03:39:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,484
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 4,347
            CDSs (with protein)               :: 4,347
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 43 of 85
            Pseudo Genes (incomplete)         :: 37 of 85
            Pseudo Genes (internal stop)      :: 19 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15604
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039579-rid11723173.denovo.118"
                     /strain="LGE2415"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8302 N 95.9628 W"
                     /collection_date="2015-08-24"
     gene            130..513
                     /gene="iraD"
                     /locus_tag="J1Y83_003231"
     CDS             130..513
                     /gene="iraD"
                     /locus_tag="J1Y83_003231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001695400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraD"
                     /protein_id="HBA7631352.1"
                     /translation="MMRQSVQTVLPESTGHNTLSLRDSVCRDLFQLFSSPHSPLPILL
                     VSGMPEWQGHNQSDKLLQSWYCRQLRSALLFHEPRIAALQVNLKAVYCHELVISLEMM
                     LYHDDEPLTFDLVWQKGSWHRTMPQ"
     gene            complement(561..2942)
                     /gene="ptsP"
                     /locus_tag="J1Y83_003232"
     CDS             complement(561..2942)
                     /gene="ptsP"
                     /locus_tag="J1Y83_003232"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016237692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HBA7631353.1"
                     /translation="MVAIVIVSHSLRLAQGIEELALQMSGGDVPLAIAAGIDDPQNPI
                     GTDAIAIMSAIESVWSPDGVLVLMDMGSALLSTEMALELLSEEQRSAIYLLAAPVVEG
                     AMSAVTSAAAGLSVTEIIAEVDLALCAKQQQLMPSSTAGEVIPAISPVNHCDEWQTFC
                     WTIRNPHGIHARPAASILKVSAQYSANIIVIKGDKRASTRSLNELAMLGVRCGDEIIF
                     QAEGTDASDALKAIETLAKNHFGEAHLLASSEIPLTETPVVSAPVDGAISGLSVQNGI
                     AIGPVKWFTCERPEITQRTVDSPQEELSRIESAIDIVVCELADKAAGPEGDIFAAHKM
                     MLEDPEITRQLQQRLAKGKQAEFAWLEVMQALAEQYCQAETLYLREREADIRDLTRQV
                     LNQLCGVSEQHFITTAPCILLANDLLPSQITSLNKAHILGICLHNGGTTSHTAILARA
                     MGIPAIAKAAITPQNVRDNDTVILDGETGRLWLQPDEVTRLDLLQRAEAWRQQRDRQL
                     ADAMLPAVTQGGRNISVLANIGDLQDIDAALSHGAEGVGLLRTEFLFHESATLPDEEE
                     QFRVYCSVARVFGDKPVTIRTLDIGGDKPLPSYPLPTEDNPFLGLRGIRLCLAHPQIF
                     IPQLRALIRAGKEYPTLQIMLPMVSTLEEVRTVKTLIHAQAKLLGLTAENLPALGIMI
                     EVPAAVMIADKLASEVDFFSIGTNDLTQYIMAADRGNSTVAKLVDYCNDAVINAIAMV
                     CQAGRNNEIPVSMCGEMAGDIQQTARLLTMGIDKLSASPSRLPALKAAIRTSH"
     gene            complement(2946..3578)
                     /gene="dhaL"
                     /locus_tag="J1Y83_003233"
     CDS             complement(2946..3578)
                     /gene="dhaL"
                     /locus_tag="J1Y83_003233"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000701650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase ADP-binding subunit
                     DhaL"
                     /protein_id="HBA7631354.1"
                     /translation="MKIQNKHVIAWLESCAAHLTEQQDFLTALDRDIGDADHGLNMNR
                     GFSAVKATLPDIERQHIGNILKNTGMKLLSSVGGASGPLYGTLFIRASAAVGARTELS
                     LEEWLACLEEGIAGVIARGKAEQGDKTLCDVWVPVLHEAKKNLQAGMSPSLLLNTMVQ
                     DAATAVDNTINMQAKKGRASYLGARSVGHQDPGATSSWLMIKAMQEGFAG"
     gene            complement(3591..4661)
                     /gene="dhaK"
                     /locus_tag="J1Y83_003234"
     CDS             complement(3591..4661)
                     /gene="dhaK"
                     /locus_tag="J1Y83_003234"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001312417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HBA7631355.1"
                     /translation="MTMKKLINQIDSVVTEQMEGLIATWPHLQANYAPRYVWCKQTDN
                     AVALISGGGSGHEPLHAGFVGMGMLTGACPGEIFTSPTPDQMIECAKAVDNGSGVLFF
                     IKNYTGDILNFETAVEMLHEEGIAVGTVIIDDDVAVKDSLYTAGRRGVAGTVFVEKIV
                     GAAALQGYNLGQCEQLGKDVNNATRSFGIALSACTVPAAGKPSFELADNEIEFGVGIH
                     GEPGIERRTLQDLNTLIDSVIAQLLDNTPWRRTLRHWDRHAGGWIDASSMNESFDQNA
                     EYIVLINGLGSTPESELYGVARVFMCAAQRQGIKISRQLVGNYCTSLDMAGFSISLLK
                     CTPEFLQLWDAPVNTPALRWGC"
     gene            4981..6063
                     /locus_tag="J1Y83_003235"
     CDS             4981..6063
                     /locus_tag="J1Y83_003235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005356517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol dehydrogenase"
                     /protein_id="HBA7631356.1"
                     /translation="MDKIIISPAKYIQGNGSLDNIATYAAILGTEPLIIADEFVTGLV
                     GDRVSQSFAKENITADFDIFCGECSQNEISRIREKFNQRKYNVVIGIGGGKTLDTAKA
                     VAYYQKIPVVVVPTIASTDAPTSSLAVIYTPDGQFSEYLFFPKNPDMVIMDTGVISAA
                     PVRLLVAGMGDALSTWFEARANQASGKATMAGGASTLAALAIARLCYTTLLEDGYKAK
                     VAVEQGVSTKAVENIIEANTYLSGIGFESSGLAAAHAIHNGMTQLEECHHCYHGEKVA
                     FGVLVQLVLENAPQEEIETVLNFCHSVGLPTNLHMLGVKEINKDKLRAVAKAATAEGE
                     TIHNMPFVVTAQDVLCAILTAHHLGL"
     gene            6130..7398
                     /locus_tag="J1Y83_003236"
     CDS             6130..7398
                     /locus_tag="J1Y83_003236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016249535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBA7631357.1"
                     /translation="MTSNWKRWITLALCGIAGSAIYKLPYLRETYYDAMQQATGATNA
                     ELGFLMTAYGLVNFLLYLPGGWAADRFSARKLMTFSLISTGISGFYYATFPSYTMICL
                     LHALWAVTTVFTFWAVCVRIIRTLGTSEEQGRLYGYWFLGKGLTSIVLGFLSVPVFAK
                     FGEGVDGLRATIIFYSVVTILAGVLAWFVCQDETHSEDKANFRLADMAFVLKMPTVWL
                     AGVVTFCMWSIYIGFGMVTPYLTQILHMGESEVAVASILRAYVLFAMGGLIGGQLADR
                     CASRTRFMIYAFIGMIVFTTVYFFLPGESRYVTIALANMVALGVFIYSANAVFFSIID
                     EVRIPAKVTGTAAGLISLLTYFPEIYCYTMVGNMVDRKPGIAGYQDVFLFMLVCAFIG
                     LIAALVLQRVNRKSKNQVNEITENNADCAS"
     gene            7416..8780
                     /locus_tag="J1Y83_003237"
     CDS             7416..8780
                     /locus_tag="J1Y83_003237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000228116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HBA7631358.1"
                     /translation="MVDMINESARQTPVIAQTDVLVIGGGPAGLTAAIAAGRLGARTM
                     IVERYGSLGGVLTQVGVESFAWYRHPGTEDCEGICREYEGRARALGFTRPEPQSISEV
                     IDTEGFKVVADQMITEAGVEPLYHSWVVDVIKEGDTLCGVIVENKSGRGAILAKRIVD
                     CTGDADIVARAGAPWTKRGKDQLMGVTVMFSCAGVDVARFNRFVAEELKPTYADWGKN
                     WTIQTTGKEDPMFSPYMEDIFTRAQQDGVIPGDAQAIAGTWSTFSESGEAFQMNMVYA
                     FGFDCTDVFDLTKAEIAGRQQALWAIDALRHYVPGFENVRLRNFGATLGTRESRLIEG
                     EIRIADDYVLNQGRCSDSVGIFPEFIDGSGYLILPTTGRFFQIPYGCLVPQKVENLLV
                     AGRCISAGVVAHTSMRNMMCCAVTGEAAGTAAVVSLQQNCTVRQVAIPDLQNTLQQQG
                     VRLA"
     gene            9081..10622
                     /locus_tag="J1Y83_003238"
     CDS             9081..10622
                     /locus_tag="J1Y83_003238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001528251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine transporter CniT"
                     /protein_id="HBA7631359.1"
                     /translation="MQYLKKRFSLIELNVFIPAILFIAVIILCLTIYPQDTSRYINKI
                     HHFLTWEMGGIFLVMTFLVVLCCLWLAFSRYGDILLGQSGEKPDFSLLTWLGLIFTSG
                     TGGSLLYLASVEWIWIIQQPPFGATAGSAQAARWASAYGMFHWGPSAWAWYLICAVPI
                     GWFMHVKKTNSLKVSDLCRGCLGARADGFCGHCVNFFYMFGLLGGAVTSLALGTPMIS
                     AVFCHVFHLDPAGQFINVMVIFIWTLVPLFILFFGLKKGVAWASNWNIRADILMLLAI
                     LICGPTAFILNQSIDGLGLMLQNFVAMSLSTDAIGRSGFPQMWTVFYFSWWVVYAIPF
                     GLFIARISKGRTIRQLIVCGTLAGSLGCMVFYMVLANFGLSLQTTHVIDFVPILNEQG
                     RGVVVSRLLEQLPASQVFLVAFGAIALISYITGHCTVGYALGFATQKRADSESEPAFW
                     NVAFWLIMTGIVAITLYLLDAQSLQPLQTVSILAGLPLCGVVFILLKSFLTQLAAEEK
                     TARDE"
     gene            complement(10678..11820)
                     /locus_tag="J1Y83_003239"
     CDS             complement(10678..11820)
                     /locus_tag="J1Y83_003239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001523874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HBA7631360.1"
                     /translation="MKIVISPDSFKECLPAWKVAEALAIGWRKVLPGSQLVCLPVADG
                     GEGTLETLIHATDGTFYTKKVTGPLGESIHAQYGILGNQTTAVIELAQASGLELVSPV
                     QRSPLYTTSFGTGELILAALEHNIDTVILCLGGSATNDGGIGLMSALGASFTDAEGLS
                     VSVNGMGLAAIHHIDLQHLDPRLKNVKFIAACDVTNPLTGDNGATRVFGQQKGASADD
                     LEQLEQGMKNYARCIYLCCGKEVDTIPGSGAAGGVGAALMAFLDARLQPGISLVLEAI
                     QYTQHLKYAALAIVGEGKLDSQSLNGKAPVGAAKVAQMMGVPVIAIAGYIDDQLDLNE
                     LRQCGIEACFSVVNGPCDLPTALSQGENNLIRLGENLAGYFRAILS"
     gene            complement(11895..12782)
                     /locus_tag="J1Y83_003240"
     CDS             complement(11895..12782)
                     /locus_tag="J1Y83_003240"
                     /EC_number="1.1.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001020468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-3-oxopropionate reductase"
                     /protein_id="HBA7631361.1"
                     /translation="MNIGFIGTGIMGKPMAYNLQQAGHTLYFSAHFEPAPQELIGERG
                     IVCSTPREVAQECEVIITMLPDTPHVEDVLFHPNYGVIHGLSHGKIVIDMSSISPVAT
                     KAFAQRIIAAGAEYIDAPVSGGEVGAKAGTLSIMVGGCEEVYLQIKPILELMGKNITL
                     VGNVGDGQTCKVANQIIVALNIEAVAEALLFASKSGADPARVREALMGGFASSRVLEV
                     HGERMIKGTFEPGFRISLHQKDLNLALENARLLNTPLPNTATTQQLFSACAALGGKEW
                     DHSALIRALETQANFTIRK"
     gene            complement(12800..13582)
                     /gene="hyi"
                     /locus_tag="J1Y83_003241"
     CDS             complement(12800..13582)
                     /gene="hyi"
                     /locus_tag="J1Y83_003241"
                     /EC_number="5.3.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001682525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxypyruvate isomerase"
                     /protein_id="HBA7631362.1"
                     /translation="MAKFAANLSMLFTEHPFIERFAQASHAGFHGVEYLFPYDFSTDE
                     LASQLHQHNLTQVLFNLPAGNWQEGERGIACHPGRAKEFQEGVCRAIEYAQALNCSQV
                     NCLAGKHPGGYSHEQCHETLVENLRYAVDKLASHGIKLVLEAINTKDIPGFFVNNTRQ
                     ALNIIHDVNHPDFRYQYDIYHMQIMEGNIATTIKNNLNNIGHIQLADNPGRHEPGTGE
                     INYPWLLNYIDQIGYQGWIGCEYIPSTTTTESLRWLKNETQF"
     gene            complement(13595..15334)
                     /gene="gcl"
                     /locus_tag="J1Y83_003242"
     CDS             complement(13595..15334)
                     /gene="gcl"
                     /locus_tag="J1Y83_003242"
                     /EC_number="4.1.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001357513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate carboligase"
                     /protein_id="HBA7631363.1"
                     /translation="MARMRAIEAAVEILKKEGISVAFGVPGAAINPLYAAMKKSGGID
                     HILARHVEGASHMAEGYTRSQNGNIGVCIGTSGPAGTDMITGLYSASADSIPILCITG
                     QAPVGKLHKEDFQAVDIEAIATPVTKMARTILEAGQLPGIFQKAFWEMRSGRPGPVLL
                     DLPFDVQMTEIEFDIDLYQPLTPWQPQATRAQAVRALEMLNDAEKPVIIAGGGIINAE
                     ASELLREFVELTGVPVIQTLRGWGALSDDHPLMIGRMGCQAGHRYGNASYLASDFVFG
                     IGNRWANRHTGAIETYTEGRKFIHVDIEPAQIGRIFAPDLGIVSDAESALTLFIQVAR
                     DMKPRGELKDRSRWIAECAERKRTMLRRSDFDCNPIKPQRVYHEMNKVFGPETRYIST
                     IGLAQIAANQFLHVYRPRHWINACQAGPLGWTMPAALGAVKADPSVPVVAISGDYDFQ
                     FLIEELAVGAQFNLPYIHILLNNAYLGLIRQSQRAFDIDYCVQLSFENINAPEINGYG
                     VDHKAVVEGLGCKAIRVFASQDIAPALQEAQRLRDEFHVPVVVEIITERVTNIAMGPD
                     INKVTEFEEILDL"
BASE COUNT         3926 a         3578 c         3837 g         4263 t
ORIGIN      
        1 aaatccctta tgaaagtgcc agggacgcaa tgagcataaa ggaatgatta tggaatatga
       61 gcatcaaccc agtggtgtcc ggcagccagg gcaggataca gatgccacta aacgtcagga
      121 gtgcgcaaaa tgatgcgcca atcagttcag acggtattac ctgaaagtac aggacacaat
      181 acgttatcgc tgcgtgattc cgtatgccgg gatctctttc aactttttag ttctccccat
      241 tcaccgctac caatattact tgtttcaggc atgcctgagt ggcaagggca taatcagagc
      301 gacaagcttc tccagagttg gtattgccgc cagttacgtt ccgcgctttt atttcatgag
      361 ccacgtattg cggcattgca ggtcaatctg aaagcagtct attgccacga gttggtcatt
      421 agtctggaga tgatgctgta ccacgatgat gagccgctaa cgtttgacct ggtctggcag
      481 aaggggagct ggcatcgcac gatgccgcag taaatgacgg gaaacgcgat acgcgcaaga
      541 cgtatcgcgt taggtcacaa ttagtgggag gttctgattg ctgctttcaa ggccggaagc
      601 cgggacggac tggcactgag cttatcaatc cccatggtga gcagtcgtgc tgtttgctgg
      661 atatctccag ccatttcgcc gcacatactg accggaattt cattattacg tccggcctga
      721 cacaccatag caatagcgtt aatcaccgcg tcattacaat aatcgaccag tttcgcgacg
      781 gtgctatttc ctctgtctgc cgccataata tattgcgtca gatcgttggt gccgatggaa
      841 aagaaatcga cttcactggc taatttgtct gcaatcatga ctgcggcagg aacctcaatc
      901 ataattccca gcgccggaag attttctgca gtaagtccca gcagttttgc ctgagcatgt
      961 atcaatgttt tgacggtcct gacctcctcg agcgttgaaa ccatcggcag cataatttgc
     1021 aacgttgggt attctttccc ggccctgata agtgcgcgca attgaggaat aaatatttgt
     1081 ggatgcgcca gacacaaccg gatacctcga agtcccagga aggggttgtc ctctgtgggt
     1141 aaagggtatg aaggtagcgg tttatcacca ccaatgtcca gagtccggat cgtgactggt
     1201 ttgtcgccaa aaactcgggc gacagaacaa tacacgcgaa actgctcttc ttcatcgggt
     1261 aatgttgcag actcatggaa taaaaactcg gtacgcagta aacccacgcc ctcagcgccg
     1321 tgagatagtg cggcatcaat gtcttgcaga tcaccaatat tcgccagcac gctgatgtta
     1381 cgcccacctt gcgtgacagc gggcagcatg gcatcagcta actgacgatc cctttgttgc
     1441 cgccatgctt cggcgcgttg cagtaaatcc aggcgggtga cctcatccgg ctgtagccac
     1501 aaacggcctg tttcaccatc aagaatgacc gtgtcgttat cgcggacgtt ttgcggggta
     1561 atcgccgctt tcgcaatagc ggggattccc attgctctgg caaggatcgc tgtatgtgaa
     1621 gttgttccgc cgttatgaag acaaatgcca agaatgtgtg ctttattcag gctggttatc
     1681 tgtgatggca acagatcgtt ggctaacaaa atgcagggcg cggtagtgat aaagtgttgt
     1741 tcacttaccc cgcaaagctg gttcagtacc tgacgtgtaa ggtcacggat atctgcctcg
     1801 cgttcacgta aatacagtgt ttccgcctga caatattgct ctgctaatgc ctgcatgact
     1861 tctaaccatg cgaattccgc ctgtttgccc tttgccagac gttgctgtaa ttgccgggta
     1921 atctctggat cttcaagcat cattttatgg gcggcaaaaa tatcgccttc agggcctgcc
     1981 gctttatcgg caagttcaca aacaacaatg tcgatagcag actcgattcg cgacagctct
     2041 tcttgcggag aatcgacggt gcgctgcgta atttctggtc gttcgcaggt aaaccattta
     2101 acaggaccaa tagcgatacc attttgtact gacaagcctg atattgcgcc atcgacaggg
     2161 gcgctgacca ccggcgtttc cgttagcgga atctctgaag aagcgagcag atgagcttca
     2221 ccaaaatgat ttttcgccaa cgtttcaatt gccttgagag cgtcactggc atcggtgcct
     2281 tcggcctgga agatgatttc atcgccacag cggacgccca gcattgccag ttcgttgaga
     2341 ctgcgggtgc tggcgcgttt atcgcctttg atgacgataa tgtttgccga atattgtgct
     2401 gatactttca ggatgctggc tgccggacga gcatgaatgc cgtggggatt acgtatcgtc
     2461 cagcaaaaag tttgccattc atcgcaatga ttgactggtg atattgctgg aataacctcg
     2521 cctgcagttg aggatggcat gagctgctgc tgtttagcgc acagcgcaag atccacttca
     2581 gcgataatct ccgtcaccga aagccccgct gcggcagacg ttaccgctga catggcacct
     2641 tccactacag gggcggcgag caggtaaatt gcacttcttt gctcttcaga aagcagctcc
     2701 agcgccatct cggtactaag cagcgcactg cccatatcca tgagtaccag aacgccatcc
     2761 ggtgaccaga ccgattcaat cgcagacata atggcaatag catcagtgcc aatcggattt
     2821 tgcggatcat caatacctgc agcaatggcc agaggaacgt cccctccaga catctgtagt
     2881 gctaattcct caatcccctg ggcgagacgt agactatgag aaacaataac aatggcaacc
     2941 ataatttatc ccgcgaatcc ttcctgcata gccttaatca tcagccagga ggaggtcgcg
     3001 cctggatcct gatggcccac actgcgagcg ccgagataac tggcacgtcc ttttttcgcc
     3061 tgcatattga tggtgttatc gacggctgtt gccgcatctt gcaccatcgt attcagcaga
     3121 agggaaggcg acattccggc ttgcaggttc tttttggctt cgtggaggac tggaacccag
     3181 acatcacaca gtgttttatc cccttgttct gcttttcctc gtgcgataac gcctgcaatc
     3241 ccttcttcca gacacgccag ccactcttcc agacttaatt ctgttctggc tcccactgcc
     3301 gcagaagcgc gaataaataa tgtgccgtaa agaggaccac tggcaccgcc aacgctggaa
     3361 agtagtttca tgccagtatt tttaaggata ttgccaatat gctgacgttc aatgtcgggc
     3421 aacgtcgctt tcacggcact gaaaccacga ttcatattga ggccgtggtc ggcatcacca
     3481 atatctctgt caagtgcggt tagaaaatct tgttgttcag taagatgtgc agcacaactc
     3541 tccagccagg caataacatg tttgttttga attttcatat aatgtcctta ttaacatccc
     3601 caacgaagcg ctggcgtatt gactggggca tcccaaagtt gcaaaaactc tggggtacat
     3661 ttcagtaggc taatggaaaa tccggccata tctaatgatg tacaataatt accgaccaac
     3721 tggcggctaa ttttaattcc ctggcgctgt gcggcgcaca taaataccct ggctacacca
     3781 tataactctg attcaggcgt gctgccaagt ccgttaatta acacaatata ttcggcattt
     3841 tgatcgaatg actcattcat tgatgatgcg tcaatccatc cccccgcatg gcgatcccaa
     3901 tggcgtaatg tgcgtcgcca gggggtatta tccagtaatt gcgctatcac gctgtcgatt
     3961 aaagtgttca gatcttgcaa agtgcggcgt tcaatgccgg gttcaccgtg gatcccgaca
     4021 ccaaattcga tttcattatc agccagttca aatgagggtt ttcccgcggc gggaaccgta
     4081 caggctgaca gtgcgatgcc aaaagaacgg gtggcattgt taacatcttt gccaagttgc
     4141 tcgcactgcc ccaggttata tccctgaagt gcagccgcac cgacaatctt ttcaacaaaa
     4201 actgtacccg caacaccgcg acgtccggct gtatacaggc tatcttttac cgctacatca
     4261 tcatcaataa tgacggtccc aacggcgatg ccttcttcgt gtaacatctc gactgccgtt
     4321 tcgaaattca gaatatcgcc agtgtaattt ttgatgaaaa acagaacgcc agaaccatta
     4381 tcaaccgctt tggcacattc gatcatttgg tctggtgtgg gcgatgtgaa gatttcacca
     4441 ggacatgcac cggttaacat tcccataccg acaaaacctg catgcagtgg ttcatggcct
     4501 gagccgccgc ctgaaatcag cgccactgca ttatcggttt gtttgcacca cacatatcgg
     4561 ggtgcgtaat ttgcctgcag gtgtggccag gtggcgatga gtccttccat ttgttctgtg
     4621 acaacagaat caatctggtt aattaatttc ttcatagtca ttcctgttct gataaattac
     4681 gtaaatcgcg ctaaatatgc ttgcgagatt aattctccag ccgcaataaa acaaatcgca
     4741 cgataataag attcataatg aaacagttaa ttcattgtgt tgttccaaaa tgaaatggta
     4801 ccagcgtgga tattgatata ttgtgattaa ggcatgaaaa gtgtttttta ttatcatctg
     4861 cagatgatgt tttttacagc gcgatcataa aacataattt ttcgcgaatg aaatgtttgc
     4921 cgccactgga tgcaacgagt aggttaacag tgaagcaaaa caagttacgc gaggaatgat
     4981 atggataaga ttattataag tccggctaaa tatatccagg gaaatggttc tctggataat
     5041 attgctacat atgcggcgat tttgggaaca gaaccattaa ttattgcaga tgaatttgtg
     5101 accggtctgg tgggtgacag agtaagccag agctttgcaa aagaaaatat tactgcagat
     5161 tttgatatct tttgtggtga atgctcgcaa aatgaaattt ctcgaattcg tgagaaattt
     5221 aatcagcgta aatataatgt cgttattggt attggcggcg gtaaaacgtt ggatacggca
     5281 aaagccgtag cgtattacca aaaaattccg gtggtggtgg tgccgacaat tgcttccaca
     5341 gatgcgccaa ccagttctct ggcagtaatt tacaccccgg atggtcagtt tagcgaatat
     5401 ctgtttttcc cgaaaaaccc ggatatggtg attatggata ctggcgtcat ttcagccgcg
     5461 ccagtgcgtc tgctggtggc cggtatggga gatgcattgt caacctggtt tgaggcgaga
     5521 gccaatcagg cgtcaggaaa agccacaatg gcaggtgggg cgtcgacact ggcggcactg
     5581 gctattgccc gactctgtta taccacgcta ctggaagatg gctataaggc aaaagtcgcg
     5641 gttgaacaag gtgttagcac caaagcggta gagaatatta tcgaagctaa tacctatctt
     5701 agcggcattg gatttgaaag tagcggcctg gcggcagctc atgccattca taacggcatg
     5761 acgcaactgg aggaatgtca tcattgctac cacggtgaaa aagtggcatt tggcgtcctg
     5821 gtacagctcg tactggaaaa cgcaccacag gaggaaattg aaaccgtact caatttctgc
     5881 catagcgttg gcttgccgac aaatcttcat atgttaggcg tgaaggaaat taataaagat
     5941 aaattacggg cagtggctaa agccgcgact gctgaagggg aaactatcca taatatgcca
     6001 ttcgtagtaa cagcgcagga cgttctttgc gcaattttaa ctgcacatca tttgggactt
     6061 taaaaaataa tacggcctgt gcaaagcagg ccgttggtgt cattttaaat acttcattcg
     6121 ggaataaata tgaccagtaa ctggaaacgt tggatcacgc tggcgttatg tggtattgcc
     6181 ggaagtgcaa tatataaatt gccttattta cgggaaacct attacgacgc gatgcagcag
     6241 gctacaggag cgactaatgc tgaacttggt tttctaatga cagcctatgg tctggtgaac
     6301 tttttattat atctacctgg cggttgggct gccgatagat tttctgcacg taaattaatg
     6361 accttttcat taatttccac cgggataagc ggtttttatt atgcgacctt cccctcgtat
     6421 accatgatct gtttgttaca tgcactttgg gctgttacaa ccgtatttac gttttgggcc
     6481 gtgtgtgtgc gaattattcg taccctggga accagcgaag aacaaggtcg cttatacggt
     6541 tactggtttc tgggtaaggg attaacctcc attgtactgg gatttctttc cgtgccggta
     6601 tttgccaaat ttggcgaagg tgttgatggt ttacgggcga caattatttt ttattccgta
     6661 gtgactattc tggccggtgt tttagcctgg tttgtttgtc aggatgaaac gcacagcgaa
     6721 gataaagcga atttccgtct ggcagatatg gcattcgtgc tgaaaatgcc gacggtttgg
     6781 ctggcaggtg ttgtgacctt ttgtatgtgg tcaatttata tcggctttgg catggtcacg
     6841 ccgtatctca cgcagatcct gcatatgggc gaatcggaag tggcagttgc cagtattctt
     6901 cgcgcctatg tgttatttgc gatgggggga ttaattggcg gacaactggc cgaccgctgt
     6961 gcgtcacgca cgcgttttat gatttacgcc tttatcggca tgattgtctt tactaccgtt
     7021 tatttcttcc tgcccggtga gtcgcgctac gtcactattg cgctggcgaa tatggttgcg
     7081 ttgggcgtgt ttatttactc tgcgaatgcc gtgttcttct caattattga tgaggttcgg
     7141 atcccggcaa aagtcacagg gactgctgcg ggtctgatct ctttactgac ctatttcccg
     7201 gaaatctact gctacacgat ggtgggcaat atggtcgacc gcaaaccggg tattgccggt
     7261 tatcaggatg ttttcctgtt catgttggtt tgcgcattca tcggccttat cgccgcactg
     7321 gtcctccagc gtgtgaatcg taaaagtaaa aatcaagtca atgaaatcac tgaaaacaat
     7381 gcggattgcg catcctgaat ctgatggggg aaccaatggt ggacatgatt aatgaaagtg
     7441 cacggcaaac gccagtcatt gcacaaacgg acgttctggt tatcggtggc ggtccggcag
     7501 gattaacggc ggcgatagcg gcggggcggc taggtgccag aaccatgatt gttgagcgtt
     7561 acggctcact aggcggcgta ttgacgcagg ttggtgtgga aagttttgcc tggtatcgcc
     7621 atccggggac ggaagattgt gaagggattt gtcgtgaata tgaaggccgc gcgcgagcat
     7681 tgggcttcac gcgaccagaa cctcagtcaa ttagcgaagt tatagatact gaaggattta
     7741 aagttgttgc cgatcagatg ataacggaag ctggcgttga gccgttatat cactcctggg
     7801 ttgtggatgt gatcaaggaa ggggatacgt tatgcggtgt tattgtcgag aacaaatcag
     7861 gacgaggggc aattctggcg aaaagaatcg tcgattgcac gggggatgct gatattgtcg
     7921 ctcgtgcagg cgcgccctgg acgaaacggg gaaaggacca actgatgggc gtcaccgtga
     7981 tgttcagttg cgcaggtgtt gatgtggcac gctttaaccg ttttgttgcg gaagaactta
     8041 agccgaccta cgcggattgg ggcaaaaact ggacgattca aaccacgggt aaagaagacc
     8101 cgatgtttag cccgtatatg gaggatattt ttacccgcgc gcaacaggat ggtgtgattc
     8161 caggtgacgc ccaggcgatt gccggaacct ggtcgacctt ttctgaaagc ggtgaggctt
     8221 tccagatgaa tatggtgtac gcctttggtt ttgactgtac cgatgtcttc gatttaacca
     8281 aagctgagat tgccggaagg cagcaagcat tatgggcaat tgacgcacta cgccactatg
     8341 ttccgggctt tgaaaatgta cggttacgca attttggtgc cacgctgggg acgcgtgaat
     8401 cacggcttat tgagggggaa atacgtattg ctgatgatta cgtccttaat caggggcgtt
     8461 gttcggacag tgtagggatt ttcccggaat ttattgatgg ttccggttat ctcattttgc
     8521 caacgaccgg gcgtttcttt cagatccctt atggttgtct ggtgccgcaa aaagtggaga
     8581 accttttggt cgccggtcgc tgtatttccg caggcgtagt tgcacatact tctatgcgta
     8641 acatgatgtg ttgtgccgtt accggtgagg ccgcaggtac tgccgccgtg gtttcgctac
     8701 agcaaaattg caccgtgcgt caggttgcta tccctgattt gcaaaacacg ctgcaacagc
     8761 agggcgtacg tctggcataa caacaaggcg cagagagctg aaagcctgtc tgcgccttgc
     8821 aggagaaaga ccatgaggga tgatgacctg gagcacttaa gtgccaatta ctggataacc
     8881 acagttctct gcggcctctt tttggggata ttcctgttca tgctgcaaca gcatcagcgt
     8941 ggtttagctc tgtattgtat tgcggctctt tcacttattt gcgccttttt ttctttacgc
     9001 ctttcccgac aattgcaaca catgaaaaag gaacggcgaa atattcgacc tcattgaatg
     9061 aaaacattca ggagactgaa atgcaatatt taaaaaaacg cttttctttg attgaactga
     9121 atgtgtttat tccagcaata ttatttattg cggtaatcat tctgtgcttg acgatatatc
     9181 cgcaagatac cagccggtat ataaataaaa tacatcattt tttaacctgg gaaatgggcg
     9241 ggatttttct ggtcatgact tttctggttg tactttgttg tctgtggctg gccttctcac
     9301 gttatggcga tattttgtta ggtcagtcgg gagaaaaacc tgacttcagc ttgttaacct
     9361 ggcttggact tatttttact tctggaacgg ggggcagctt gttatatctg gcctctgtag
     9421 agtggatttg gatcattcag caaccgcctt ttggcgcaac agcaggaagc gctcaggctg
     9481 ctcgttgggc ctctgcttat ggcatgttcc attgggggcc gtccgcatgg gcatggtatt
     9541 tgatttgcgc cgttcctatt ggttggttta tgcatgttaa gaaaacaaac tcattaaagg
     9601 tcagtgattt atgtcgtggg tgtttgggag cgcgtgcgga tggtttttgc gggcattgtg
     9661 tgaatttttt ctacatgttt ggtttgctcg gcggcgcggt aacgtctctg gcgctgggaa
     9721 cgccgatgat ttctgccgta ttttgccatg tgttccatct ggatcctgcc gggcagttta
     9781 tcaatgtcat ggttattttt atctggacgc tagtaccatt attcattctc ttttttggac
     9841 ttaaaaaagg tgtggcatgg gccagtaact ggaatattcg tgctgacatt ttgatgctac
     9901 tggcaatact gatttgtgga ccaacggcat ttatacttaa tcaatcaatt gatggtctcg
     9961 gcctgatgct gcaaaatttt gtagcgatga gtttaagtac cgatgctatt ggtcgtagcg
    10021 gatttccaca gatgtggacc gtattttatt tttcatggtg ggtcgtgtat gccatcccat
    10081 tcggtttatt tatcgcccgt atttcaaaag gaaggacgat ccggcaattg attgtatgtg
    10141 gaactctggc aggttcattg ggatgcatgg ttttttacat ggtactggct aatttcggct
    10201 tatcgttgca gacaactcat gtcatcgatt ttgttcccat acttaacgaa caagggcgag
    10261 gcgttgtcgt ttctcgttta ctggagcagc tacccgcaag tcaggtgttt ttggttgctt
    10321 ttggggctat agcattaatt tcatatatta ccggacactg tactgtgggt tatgccctcg
    10381 gttttgcgac gcaaaaacgg gcagatagtg agagtgaacc ggcattctgg aacgtggcat
    10441 tttggttgat tatgactgga atcgtcgcca tcacactcta tcttcttgat gcgcagagtt
    10501 tgcagccgct acaaacggtc tctatcctgg ccggactacc gctttgcggc gtagtgttta
    10561 ttttattgaa gagttttttg acacagcttg cggctgaaga gaaaaccgcg agagatgaat
    10621 aatgatgttc aggaatgccc ggtaatttgc attatcgggc attttaccga acgggaatta
    10681 actcaggatc gcacgaaaat atcccgccag attttcccca agacgaatta aattattttc
    10741 tccctgactc agcgcggtgg gtaaatcaca aggaccattg acgacggaaa aacaggcttc
    10801 tattccacac tggcgtaact cattcagatc aagttgatca tcgatatacc cggcaattgc
    10861 gataaccgga acgcccatca tctgggcaac tttggctgcg cctacgggcg ctttgccatt
    10921 caggctctga ctatctaatt tcccttcacc gacaatggcc aatgctgcgt attttaaatg
    10981 ttgggtatat tgaatcgctt ccagcacgag cgaaataccc ggttgtaagc gagcatcgag
    11041 aaaagccatc aaggccgcgc caacgccgcc agccgcccca gaaccgggta tcgtatcgac
    11101 ttctttacca caacaaaggt agatgcaacg ggcatagttt ttcattccct gttccagttg
    11161 ctcaaggtcg tcagcactgg cccctttttg ttgaccgaaa acccgggtcg cgccgttatc
    11221 gccggttagt gggttggtga catcgcaggc tgcaataaat ttcacatttt tcaatcgtgg
    11281 atcgaggtgc tgtaagtcaa tgtggtgaat tgccgccagc cccatcccat taacagagac
    11341 tgatagccct tcggcgtctg taaacgatgc gccaagtgcc gacatcaacc caataccgcc
    11401 atcatttgta gcactgccac ccaggcatag aataacggta tcaatattgt gttccagggc
    11461 ggcgagaata agttcgccag tgccaaacga cgtcgtataa aggggagaac gctggacagg
    11521 agaaaccagt tccagccctg aagcttgtgc cagttctatc actgcggtgg tttggttgcc
    11581 taaaataccg tattgcgcat gtattgattc gccaagcggt ccggtgactt ttttagtgta
    11641 aaacgtaccg tcagtcgcat gaatgagtgt ttcgagcgtg ccttcgccgc cgtcagccac
    11701 gggcaaacac accagttggc tgccaggcag gaccttgcgc cagccgattg ccagcgcttc
    11761 ggcgactttc catgcaggaa ggcattcttt aaaggagtcg ggggagataa ctatcttcat
    11821 agcaggtctc tcaaaatagg ggaatggggc agggcaatga gtgaacgaag ggaaccggtg
    11881 gttcccttaa ttcattattt acggatagtg aaattggctt gtgtttcaag cgcccgaatt
    11941 aacgcagaat gatcccactc tttaccaccg agcgcggcgc aggcgctaaa taactgctgt
    12001 gtcgtcgccg tgttaggaag cggggtattc aacaggcggg cattctccag cgccagattc
    12061 agatcttttt gatgcagact aatacgaaaa ccgggttcga atgttccttt aatcatccgt
    12121 tcgccatgaa cttccagcac gcgagaggag gcaaaccctc ccatcagcgc ttcacgtacg
    12181 cgggcgggat cggcacctga tttactggca aagagcaggg cttcagccac cgcttcaata
    12241 ttgagtgcaa caataatttg gttagcgact ttgcacgttt gaccatcacc gacgttaccg
    12301 accagagtga tatttttacc catcaattcc agaatcggtt tgatctgcaa ataaacctct
    12361 tcacatccgc caaccataat ggaaagcgtc ccggctttcg cgccaacttc accgccagag
    12421 acgggcgcat cgatatattc cgcgcctgcc gcgataatac gttgtgcaaa tgctttggtg
    12481 gcaaccgggg aaattgaact catatcaatc acaattttcc cgtggcttaa accgtgtatt
    12541 acgccatagt tgggatgaaa aagaacatcc tcaacatgtg gggtatcagg gagcatggtg
    12601 atgatgacct cacactcctg cgcgacttca cgcggtgtag agcaaacaat gccacgttcg
    12661 ccaataagct cttgcggtgc aggttcaaaa tgcgctgaaa aataaagagt atgtccggct
    12721 tgttgtaaat tataagccat cggcttaccc attatgccgg tgccgataaa tccaatattc
    12781 atgataaata cctcagggat taaaattgag tctcgttttt tagccagcgt agactttcag
    12841 tcgttgtcgt agatggaata tattcgcaac caatccatcc ctgataacca atctggtcta
    12901 tataatttaa caaccacggg taattaattt ctcccgtccc tggttcatga cgcccagggt
    12961 tatcggctaa ttgaatatgt ccaatgttat tcaggttgtt tttgattgtt gtagcaatat
    13021 tcccttccat gatttgcata tgataaatat cgtattgata gcgaaaatca ggatgattta
    13081 catcatgaat aatattgaga gcttgtctgg tgttgttaac aaaaaaaccg ggaatatctt
    13141 ttgtattaat ggcttccaga actaacttta tgccatgaga agccaattta tctactgcat
    13201 agcgcaaatt ttcgaccagt gtttcatgac attgttcatg cgaataacca cctggatgtt
    13261 ttcctgcaag gcaattaact tgtgaacaat taagggcctg ggcatattct atcgctctgc
    13321 aaacaccctc ctgaaattct tttgcccgac caggatgaca tgcaataccc cgttcaccct
    13381 cttgccagtt acccgcagga agattaaaca aaacctgagt cagattatgt tgatgcagtt
    13441 gcgaagccag ctcgtcagtg gaaaagtcat agggaaaaag atattcaacg ccgtggaatc
    13501 ctgcgtgact tgcctgcgca aaacgttcaa taaatggatg ctcggtaaaa agcattgata
    13561 aattcgctgc aaactttgcc ataatcctgt ttccttataa atcgagaatt tcttcaaatt
    13621 ctgtgacttt attaatgtca ggtcccatag cgatattagt cacgcgttca gtaataattt
    13681 ctacaacaac aggtacgtga aattcgtcac gcaaacgctg ggcttcttgc agtgcaggcg
    13741 caatatcctg tgatgcgaat acgcgaatcg ctttacatcc taatccttca accacagctt
    13801 tatgatcaac gccataacca ttaatttctg gagcattaat attttcaaat gacagttgaa
    13861 cgcaataatc aatatcaaat gcacgttgtg attgacgaat caaaccaaga taagcgttgt
    13921 taagtaaaat atggatatac ggaagattaa attgtgcgcc gacggctaat tcttcaatca
    13981 agaattgaaa atcgtaatca ccggatattg ccacaacggg aacagatgga tcagctttta
    14041 ccgcgcccag tgctgcgggc attgtccagc ctagcggccc agcctgacag gcattaatcc
    14101 agtggcgtgg gcggtaaaca tgtaaaaatt ggtttgcggc gatttgcgcc agaccaattg
    14161 tcgaaatata gcgtgtctcc ggtccgaaaa ccttattcat ttcgtgatac acgcgctgcg
    14221 gtttaattgg attgcagtca aagtctgaac gacgaagcat tgtgcgttta cgctcggcac
    14281 attcggcaat ccagcgactg cggtctttca gttctccacg tggcttcata tcacgggcga
    14341 cctgaataaa tagcgttaag gcgctttcag catcagaaac aatgcccaga tccggcgcaa
    14401 atatccggcc aatttgtgca ggttcgatat caacatgaat gaatttgcgg ccttcggtgt
    14461 aggtctcaat ggctccggta tggcggtttg cccaacggtt accgatacca aaaacaaaat
    14521 ctgaggccag ataactggcg ttaccatagc gatgacccgc ctgacatccc atgcgaccaa
    14581 tcattagggg gtgatcgtct gataaagcgc cccaaccgcg taaggtttga atgacgggaa
    14641 cgccagttaa ctcgacaaac tcacgcaaca attcactcgc ttcggcgtta ataatgccac
    14701 cgccagcgat gatgacgggc ttttcggcgt catttagcat ttctaatgcg cgtactgcct
    14761 gggcccgtgt tgcctgaggt tgccaggggg ttagtggctg atagagatcg atatcaaact
    14821 caatttcggt catctgaaca tcaaaaggga gatctaaaag aaccgggcca gggcggcctg
    14881 aacgcatttc ccagaaggct ttctggaata tccccggcaa ctgacccgct tcgagaattg
    14941 tgcgagccat tttggttact ggcgtggcga tagcctcaat atcgacggcc tggaaatctt
    15001 ctttatgaag ttttcctacg ggagcttgac cagtaatgca gagaatagga atggaatccg
    15061 cagaggcaga atacagcccg gtaatcatat cagtgcccgc agggcctgat gtgccaatac
    15121 acacgccaat attgccattc tgactgcggg tgtaaccttc agccatatgc gaggcgccct
    15181 caacatgacg ggccagaata tgatcaatac cgcctgattt tttcatcgcg gcatagagtg
    15241 ggtttattgc tgcgccgggt acaccaaaag caacgctaat gccttctttt ttcagtattt
    15301 ctactgcagc ttcgattgca cgcatcctcg ccatacatta tttctcctga tatttacgct
    15361 gagttgaata gtcagtcaat tttggaattt aacgatgaaa tatctttgag gaaataaaag
    15421 ggcagaacaa atttcgctgc actgtttcac agtgaaacaa ctcgtatgcc tatgttgttt
    15481 cagtttgaat aattataaat atcaaacgca aagatttcag atgttgttgg cagttttaaa
    15541 atttatctaa tgtatcattc gttttttatt gctttaacgg ttattatttc taatgtatgt
    15601 gtga
//
LOCUS       DADSYM010000118        15540 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2415
            SAMN08039579-rid11723173.denovo.119, whole genome shotgun sequence.
ACCESSION   DADSYM010000118 DADSYM010000000
VERSION     DADSYM010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039579
            Sequence Read Archive: SRR6362533
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15540)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15540)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980162.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 03:39:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,484
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 4,347
            CDSs (with protein)               :: 4,347
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 43 of 85
            Pseudo Genes (incomplete)         :: 37 of 85
            Pseudo Genes (internal stop)      :: 19 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15540
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039579-rid11723173.denovo.119"
                     /strain="LGE2415"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8302 N 95.9628 W"
                     /collection_date="2015-08-24"
     gene            complement(2..283)
                     /locus_tag="J1Y83_003243"
     CDS             complement(2..283)
                     /locus_tag="J1Y83_003243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YehE family protein"
                     /protein_id="HBA7631364.1"
                     /translation="MNKYWLSGIIFLAYGLASPAFSSETATLTINGRISPPTCSMAMV
                     NGQPQQHCGQLTYNVDTRHQVSSPVKGVTTEVIAADSDSKRRIVLNRYD"
     gene            complement(546..1655)
                     /gene="apbC"
                     /locus_tag="J1Y83_003244"
     CDS             complement(546..1655)
                     /gene="apbC"
                     /locus_tag="J1Y83_003244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster carrier protein ApbC"
                     /protein_id="HBA7631365.1"
                     /translation="MNEQSQAKSPEALRAMVAGTLANFQHPTLKHNLTTLKALHHVAW
                     MDDTLHVELVMPFVWHSAFEELKEQCSAELLRITGAKAIDWKLSHNIATLKRVKNQPG
                     INGVKNIIAVSSGKGGVGKSSTAVNLALALAAEGAKVGILDADIYGPSIPTMLGAENQ
                     RPTSPDGTHMAPIMSHGLATNSIGYLVTDDNAMVWRGPMASKALMQMLQETLWPDLDY
                     LVLDMPPGTGDIQLTLAQNIPVTGAVVVTTPQDIALIDAKKGIVMFEKVEVPVLGIVE
                     NMSVHICSNCGHHEPIFGTGGAEKLAEKYHTQLLGQMPLHISLREDLDKGTPTVISRP
                     ESEFTAIYRQLADRVAAQLYWQGEVIPGEISFRAV"
     gene            1787..3820
                     /gene="metG"
                     /locus_tag="J1Y83_003245"
     CDS             1787..3820
                     /gene="metG"
                     /locus_tag="J1Y83_003245"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HBA7631366.1"
                     /translation="MTQVAKKILVTCALPYANGSIHLGHMLEHIQADVWVRYQRMRGH
                     EVNFICADDAHGTPIMLKAQQLGITPEQMIGEMSQEHQTDFAGFNISYDNYHSTHSEE
                     NRQLSELIYSRLKENGFIKNRTISQLYDPEKGMFLPDRFVKGTCPKCKSPDQYGDNCE
                     VCGATYSPTELIEPKSVVSGATPVMRDSEHFFFDLPSFSEMLQAWTRSGALQEQVANK
                     MQEWFESGLQQWDISRDAPYFGFEIPNAPGKYFYVWLDAPIGYMGSFKNLCDKRGDSV
                     SFDKYWKKDSTAELYHFIGKDIVYFHSLFWPAMLEGSNFRKPTNLFVHGYVTVNGAKM
                     SKSRGTFIKASTWLNHFDADSLRYYYTAKLSSRIDDIDLNLEDFVQRVNADIVNKVVN
                     LASRNAGFINKRFDGVLASELADPQLYKTFTDAAEVIGEAWESREFGKAIREIMALAD
                     LANRYVDEQAPWVVAKQEGRDADLQAICSMGINLFRVLMTYLKPVLPKLTERAEAFLN
                     TELTWDGIQQPLLGHKVNPFKALYNRIDMKQVEALVEASKEEVKATAAPVTGPLADDP
                     IQETITFDDFAKVDLRVALIENAEFVEGSDKLLRLTLDLGGEKRNVFSGIRSAYPDPQ
                     ALIGRHTIMVANLAPRKMRFGISEGMVMAAGPGGKDIFLLSPDAGAKPGHQVK"
     gene            3961..7764
                     /locus_tag="J1Y83_003246"
     CDS             3961..7764
                     /locus_tag="J1Y83_003246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016242518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WGR and DUF4132 domain-containing protein"
                     /protein_id="HBA7631367.1"
                     /translation="MRHFIYQDEKSHKFWAVEQQGNELHISWGKVGTNGQNQVKSFAD
                     AVAAEKAELKLIAEKTKKGYVEGNSANVHIPPVTKESIPSEVKTAVVSKNKHPWLADD
                     AAIPVTDDINRFAFPHRSRPREINYLHKDGQIWKRIANNTRAYDPDNNYRSYPENWQQ
                     AFAELQSRILDNQQTGSAQSDAALLWSFWNSYSEDELVDDLVIRCGLESAVEIALFAL
                     QLKYKPVKGAVTTIIPPGHKAESLPSWHQRLCHHLSLASEDEWQRCVNKVLTAIPTLS
                     PARQPFAALLLPERPDIANAMALRYADQNVPAMTWLRLVVTDDAALTAAEKYDFPPLY
                     RDFRAYLAMLLANNGVSGVSRILTEFVEEHSDNPTYLFERISETEDLVKWLWKTNHPD
                     AIQILILGVIGKKKHLEYLSKACQKHSAAAIAAYATLLAIHEDKEWHKALVKLITITP
                     ELVTDVIPRVNAKAAGILSECRTQSVAEECEYATADMLPELFVAPPWVINKKKNVIPV
                     FDLPVLPIPAVTDITPGITELISHTDISRFSEIAQYQASQQTLFTDLPPIKKERWETT
                     FIPLTPEQQILWRLGFKEWRRTGEEQYEKKIMPQSAVDALLRFDFPALKAEFAQYHNK
                     GSRHWQLYALCFLPTQHAISFLDQIINEEQFSGEREILAIFGDAAIPAFMKCLQRKPQ
                     QLWIFTLFLGVSELALPMAQRLQKKMSAEDARKWLANFPRHAAAGLLPVALGKKGKDR
                     DCARQALRLLVNLNQRETIEEIARGYNQPDVLAALATLFDSDPLEEYPAKIAPLPGFY
                     QFTLWRRPRLKSNNLPLPDDAMRHLGTMLSFPRDITAYAGLDIIRETFTRESLADFGW
                     DLYTAWTEAGAPAKENWAFTALGILGNDDTARKLTPLIRAWPGESQHKRAVSGLDVLA
                     DIGSDVALMLLNGIARKIKFKALQEHAREKINIVAENRGLTMAELEDRLAPDLGLDSS
                     GSLTLDFGPRQFTVGFDETLKPVVRDANGKVLKDLPKPNQSDDKTLATDAVNLFKQLK
                     KDVRAIASQQIDRLEQAMCQCRRWTAEQFRLFLVEHPLVRHLTRRLLWGVYNNENALI
                     TCFRVAEDSTYSDAQDELFTLPAGNIGIPHVLEISPESAAAFRQIYIDYELLPPFQQL
                     DRGCYHLADNERDTHELSRWDGRLCQAGRIVGLERRGWQRLEESGSVYAMRKSTPYGA
                     LELETEHFSLIYGETGYSDLLPVESVKITSPGERYSTQPSLTFSALDAITASELINDI
                     ESLFD"
     gene            7777..11565
                     /locus_tag="J1Y83_003247"
     CDS             7777..11565
                     /locus_tag="J1Y83_003247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001215568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WGR and DUF4132 domain-containing protein"
                     /protein_id="HBA7631368.1"
                     /translation="MKTYIYQDEKSHKFWAVEQQGNELNISWGKVGTNGQSQVKSFAD
                     AAAAEKAELKLIAEKTKKGYVEQAENAPSKHTRTVTKSLELSDFSTIIQEQPSVTPEI
                     YVADKNTDVILPWLAKDIAVVFPLEVVPTTLSHRRFPGDPVTPVDKLTQLRRLACGVS
                     QRDNKTATFDFSVCTLEWQNFIAQAIDLINSPKTTKLPSPVMAVLTALELHCTRYKER
                     EDVMDQIIQEGGLEYATDVIIHLQQISIEWDYVNNNIVFLSSGISPDYLQQYSSFELR
                     LRKHLSLAEESLWQKCAQKLIAAVPHIPEWRQPLIALLLPEKPEIAHEIAQRLLGRKK
                     LPSLEWLKIVATDEHILASLEKYHEPYAIFDDYYCGAIWSATVLQEQGVAALPRFAPY
                     AASDYCADVLRHINHPFALTLLIRVAGHTKRCHDRMTKACAAFPHAALAALAELLVQK
                     EENSWRIMLMTMLISQPTLAEQVIPWLSTPAVAVLKSCQQQLTQPSNHASADLLPAIV
                     VSPPWLSKKKKSPIPVLDLAPLNLESICTITDTEAKEFQTHWDWEPHKPGEGAKNFLY
                     SLGYRRWDFDTYKYIGASDSAIDAWEREDFATLIQMFKAHHAPYQGEWHLNSLPFLPM
                     QKAIKLWEFLSKEPHTAIKPVMLYLRLAGMSGFLHSFSRYPQEGFAVANYFAATELAP
                     AVARAFNKLKTLRQDASSWLLKYPQHAITGLLPAALGKAGEAQDNARAALRMLTENGH
                     QPLLQEIARRYNQPEVTDAMNALLALDPLDNHPTKIPTLPTFYQPSLWTRPLLKANAQ
                     SLPDSALLHLGEMLRFPQEEALYPGLLQVKDACTTDSLAEFAWDLFTAWQTAGAPSKE
                     SWAFTALGVLGNDDTARKLTPLIRAWPGESQHKRATVGLDILAAIGSDIALMQLNGIA
                     QKLKFKALQERAKEKIADIAESRELTVAELEDRLAPDLGLDDNGSLLLDFGPRQFTVS
                     FDETLKPFVRDVSGSRLKDLPKPNKSDDESQANDAVNRYKLLKKDARTVAAQQVARLE
                     SAMCLRRRWSPENFQLFLVEHPLVRHLTRRLIWGVYSAENQLLACFRVAEDNSYSTAD
                     DDLFTLPEGDISIGIPHVLEISPTDAAAFGQLFADYELLPPFRQLDRNSYALTEAERN
                     ASELTRWAGRKCPSGRVMGLANKGWIKGTPQDAGWIGWMINPLGRWSLIMEIDEGFAV
                     GMSPAELSAEQILSKLWLWEGKAESYGWGSNSTQEAQFSVLDTITASELINDIEALFE
                     "
     gene            11575..15207
                     /locus_tag="J1Y83_003248"
     CDS             11575..15207
                     /locus_tag="J1Y83_003248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4132 domain-containing protein"
                     /protein_id="HBA7631369.1"
                     /translation="MDKELPWLADNAQLELKYKKGKTPLSHRNWPGEPVPVITENIIQ
                     TLGDELLQKAEKKKNIVWRYENFSLEWQSAITQAINLIGEHKPSITARTMATLACIAQ
                     NDSQQLLDEIVQQEGLEYATEVVIARQFIVRCYESDPLVVTLQYQNEDYGYGYRSETY
                     NEFDLRLRKHLSLAEESCWQRCADKLIAALPGITKVRRPFIALNLPEKPEIANELVSL
                     ECSQTHFRSKEWLKVVADDSKAVKELARYWSQDIFSDREASYMSHENHFGYAACAALL
                     REQGLAAVPRLAMYAHKEDCGSLLVQINHPQVIRTLLLVADKNKPSLQRVAKYSKNFP
                     HATLAALAELLALKAPPARPGYPIIEDKKLPAQQKARDEYWHTLLQTLMASQPQLAEE
                     VMPWLSTQAQAVVNNYLSTSPKPVIDGTDNSQMPEILVSPPWRSKKKMTAPRLDLAPL
                     ELTPQVYWQPGERERLAATESARYFSTGSFTERMERKSGRVVLQELGFGDDVWLFLNY
                     ILPGKLDAARNSLIVQWHYYQGRVEEILHGWNSPEAQLAEQALRSGHVEALINIWEND
                     NYSRYRPEKSVWNLYLLAQLPREMALTFWLRINEKKHLFAGEDYFLSILGLDTLPGLL
                     LAFSHHPKETFPLILNFGATELALPVARVWRRFAAQRDLARQWILQWPEHTATALIPL
                     VFTKPSDNSEAALLALRLLYEQGHGELLQTVANRWQRTDVWPALEQLLKLGPIEIYPA
                     RIPKAPDFWHPAMWSRPRLIKNNQPVTDDALEIIGEMLRFTQGGRFYSGLEQLKTFCQ
                     PQTLAAFAWDLFTAWQQAGASAKDNWAFLALSLFGDESTARDLTTQILAWPQEGKSAR
                     AVSGLNILTLMNNDMALIQLHHISQRAKSRSLRDNAAEFLQVVAENRGLSQEELADRL
                     VPTLGLDDPQALSFDFGLRQFTVRFDENLNPVIYDQQNVRQKSVPRLRADDDQLKAPE
                     ALARLKGLKKDATQVSKNLLPRLEAALRITRRWSLADFHSLFVNHPFTRLVTQRLIWG
                     VYPANEPRRLLNAFRVAAEGEFCNEQDEPIDLPADALIGIAHPLEMAAEMRSEFAQLF
                     ADYEIMPPFRQLARRTVLLTPDESASNSLTRWEGKSATVGQLMGMRYKGWESGYEDAF
                     VYDLGAYRLVLKFSPGFNHYNVDSKALMSFHSLRVYRDNKSVTFAELDVFDLSEALSA
                     PDVIFH"
     gene            15268..>15540
                     /locus_tag="J1Y83_003249"
     CDS             15268..>15540
                     /locus_tag="J1Y83_003249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA7631370.1"
                     /translation="MIVQKELVAIYDYEIPAPEDPFSFRLEIHKFSELFTGSVYRLER
                     FRLHPTFNDADSLINDALIYIRDDFIDERKLRGESHETVIAIFNCEL"
BASE COUNT         4132 a         3924 c         3766 g         3718 t
ORIGIN      
        1 attaatcgta gcgatttaac acgattcgac gtttgctatc gctatctgca gcaatcactt
       61 ccgtcgttac acctttaact ggcgaagaaa cctgatgacg ggtatcaaca ttatacgtta
      121 actgaccaca atgctgttga ggttgaccat taaccatagc catactgcat gttgggggac
      181 tgatcctacc attaatcgtt aatgtagcag tttctgaaga gaacgcggga gaagccaatc
      241 cataggcaag aaaaataatt cctgacagcc agtacttatt cattgaacgt tatccctgta
      301 gtaaaggtta tgcctggaaa gaatgagtgc ataacaaact atagctgtac atccactaca
      361 cagccacgaa ggatgataat gaagcatcgc cggtacgatc aatcgacttt gtagtgtttc
      421 ggacgaaggt ccgcagaata ttcgcagcat taaataagtg ttcattaatg ttgcaaaaac
      481 aaatacaccg catcaatgta acatctctgc ggtgtatgaa gaataatcag aatggttaat
      541 acgcgttaga ccgcgcggaa ggaaatctcg cctggaatga cttcaccctg ccagtagagc
      601 tgagctgcaa cgcggtcagc cagctggcgg tagatagcgg taaattcgct ctcaggacgg
      661 ctgataaccg ttggcgtgcc tttatcgaga tcctcacgta aggagatgtg taacggcatc
      721 tgccccagca gctgcgtgtg gtatttctcg gccagtttct ctgcaccacc ggtaccgaaa
      781 atcggttcat ggtgaccaca gttactgcaa atatgcacac tcatgttctc gacaataccg
      841 agcaccggaa cttcgacttt ctcgaacatc acaatgcctt ttttcgcatc gatcaacgcg
      901 atgtcctgcg gggtggtgac cacgactgca ccggttactg gaatgttttg tgccaatgtc
      961 aattgaatgt caccggtacc tggcggcata tcaagcacca gatagtcgag atccggccac
     1021 aaggtttcct gcaacatttg cattaacgcc ttgctcgcca ttgggccacg ccacaccatc
     1081 gcattgtcgt ctgtcaccag ataaccaatt gaattagttg ccaggccatg agacataatc
     1141 ggtgccatat gggtaccgtc cggcgaagtt ggacgctgat tttctgcgcc cagcatggta
     1201 gggattgatg ggccgtagat atcggcgtcc agaatgccga ctttcgcccc ttccgccgcc
     1261 agtgccaacg ccaggtttac cgccgtggag gatttaccca cgccaccttt accggaactg
     1321 accgcgataa tgttcttcac gccgttaatt cccggctgat tcttcacgcg tttcagagtg
     1381 gctatgttat gcgacagttt ccagtcgata gctttcgctc cggtgatgcg cagcagttcg
     1441 gcgctacatt gctcttttaa ctcttcgaac gcgctatgcc agacgaatgg cataaccagt
     1501 tccacatgca gcgtgtcgtc catccaggcg acatggtgca acgctttcag cgttgtgagg
     1561 ttatgcttca gggttgggtg ctgaaaattg gccagtgtcc cggcgaccat tgcgcgcaag
     1621 gcttctggtg atttggcctg ggattgttcg ttcatcccga ctcctttttt gtatagataa
     1681 accatcagct gatagtttac ctgaagaata tagagaagta cttacttaac attttcccat
     1741 ttggtactat ctaagccctt ttcactatta agaagtaatg cctactatga ctcaagtcgc
     1801 gaagaaaatt ctggtgacgt gcgcactgcc gtacgctaac ggctcaatcc acctcggcca
     1861 tatgctggag cacatccagg ctgatgtctg ggtccgttac cagcgaatgc gcggccacga
     1921 ggtcaacttc atctgcgccg acgatgccca cggcacgccg atcatgctta aagctcagca
     1981 gcttggtatc accccggagc agatgattgg cgaaatgagt caggaacatc agactgattt
     2041 cgcaggcttt aacatcagct atgacaacta tcactcgacg cacagcgaag agaatcgcca
     2101 gttgtcagaa cttatctact ctcgtctgaa agaaaacggt tttattaaaa accgcaccat
     2161 ctctcagctg tacgatccgg aaaaaggcat gttcctgccg gaccgttttg taaaaggtac
     2221 ctgcccgaaa tgtaaatccc cggatcaata cggcgataac tgcgaagtct gcggcgcgac
     2281 ctacagcccg actgaactga tcgagccgaa atcggtggtt tctggtgcta ccccggtaat
     2341 gcgtgattct gaacacttct tctttgatct gccctctttc agcgaaatgc tgcaggcatg
     2401 gacccgcagc ggtgcgttgc aggagcaggt ggcaaataaa atgcaggagt ggtttgaatc
     2461 tggcctgcaa cagtgggata tctcccgcga cgcgccttat ttcggttttg aaattccgaa
     2521 cgcgccgggc aaatatttct atgtctggct ggacgcaccg attggctata tgggctcttt
     2581 caagaatctg tgtgacaagc gcggcgacag cgtaagcttc gataaatact ggaagaaaga
     2641 ttccactgcc gagctgtatc acttcatcgg taaagatatc gtttacttcc acagcctgtt
     2701 ctggcctgcc atgctggaag gcagcaactt ccgcaagccg accaacctgt ttgttcacgg
     2761 ctatgtgacg gtgaacggcg cgaaaatgtc caagtctcgc ggcaccttta ttaaagccag
     2821 cacctggctg aatcattttg acgctgacag cctgcgttac tactacactg cgaaactctc
     2881 ttcgcgcatt gatgatatcg atctcaacct ggaggatttc gttcagcgag tgaatgccga
     2941 tatcgtgaac aaagtggtta acctggcgtc ccgtaacgct ggctttatca ataagcgttt
     3001 tgacggtgtg ctggcaagcg agctggctga cccgcagttg tacaaaacct tcactgatgc
     3061 cgctgaagtg attggtgaag cgtgggaaag ccgcgaattt ggtaaagcta tccgtgaaat
     3121 catggcactg gctgacctgg ctaaccgcta tgtcgacgag caggctccgt gggtggtggc
     3181 gaagcaggaa ggtcgcgatg ccgatctgca ggcgatttgc tcaatgggca tcaacctgtt
     3241 ccgcgtgctg atgacttacc tgaaaccggt actgccgaaa ctgactgaac gtgcagaagc
     3301 attcctgaat acagaactga cctgggatgg tatccagcaa ccgctgctgg gccataaagt
     3361 gaatccgttc aaggcactgt ataaccgtat cgatatgaaa caggtggaag cactggtgga
     3421 agcatccaaa gaagaagtga aagccacagc cgcaccggta actggcccac tggctgacga
     3481 cccgattcag gaaaccatca cctttgacga cttcgccaaa gttgacctgc gcgtggcgtt
     3541 gattgaaaac gcagagtttg tagaaggttc tgacaaactg ctgcgcctga cgctggatct
     3601 cggcggtgaa aaacgcaatg tcttctccgg cattcgttct gcttacccgg atccgcaagc
     3661 actaattggt cgtcacacca ttatggtggc taacctggca ccacgtaaaa tgcgcttcgg
     3721 tatctccgaa ggcatggtaa tggctgctgg ccccggcggg aaggatatct tcctgttaag
     3781 cccggatgcc ggtgctaaac cgggtcatca ggtgaaataa tcccccttta aggcgctgca
     3841 ttgacagcgc cttttcttta taaattccta aagttgtttt cttgcgattt tgtctctctc
     3901 taacccgcat aaatactggt agcatctgca ttcaactgga taaaattaca gggatgcaga
     3961 atgagacact ttatctatca ggacgaaaaa tcacataaat tctgggcggt ggagcagcaa
     4021 ggcaacgagt tacatatcag ttggggcaaa gtcggcacta acgggcaaaa ccaggtaaaa
     4081 agctttgctg atgctgtggc agcggaaaaa gcggagctga agctaattgc ggagaaaacg
     4141 aaaaaggggt atgtggaagg caattcggca aacgtgcata tcccgccagt cacaaaagaa
     4201 agtatccctt ctgaagttaa aactgctgtt gtaagtaaaa acaaacaccc atggctggca
     4261 gatgatgcag ccattccggt aaccgacgat attaatcgat ttgcttttcc acatcgctct
     4321 cgccccagag agatcaatta tctgcacaaa gatggtcaaa tatggaagcg catcgcgaat
     4381 aacaccaggg catatgaccc cgacaataat taccgctctt atcctgaaaa ttggcagcaa
     4441 gcgtttgccg agttacaaag tcgaattctg gacaatcaac aaacagggag tgcgcaatct
     4501 gatgcggcat tgctctggag tttttggaac tcttactctg aagatgaact ggtcgatgac
     4561 ttagtcattc gctgtggtct ggaaagtgcc gttgaaatcg ccctttttgc tcttcaactc
     4621 aaatataaac cagtaaaagg cgcagtcacc accatcattc cacctggtca caaagcagaa
     4681 tcgctaccga gttggcatca acgtctatgt catcatcttt cactcgcttc agaagatgag
     4741 tggcaacgct gtgtgaataa agtgcttact gctattccga cgctatcccc agcacggcaa
     4801 ccttttgcgg cgttactgct ccctgaacgc ccggatattg ccaatgctat ggctctacgt
     4861 tacgcagacc aaaacgttcc ggcgatgacc tggttaagac tggtagtaac cgatgatgcc
     4921 gctctgactg cagcagaaaa atacgacttc ccaccgttat atcgtgattt tcgcgcttat
     4981 ctggcaatgt tgctggcaaa taatggggtg agcggtgtaa gccggatact tactgagttt
     5041 gtggaagagc attctgacaa tcccacttat ctcttcgagc gcatcagcga gactgaagat
     5101 cttgtcaaat ggttatggaa aacgaatcac ccggatgcga ttcaaattct gatcctcggt
     5161 gtaattggca agaaaaagca cctggaatac ttaagcaaag cctgtcaaaa acattccgct
     5221 gcggctattg ccgcttatgc aactttgctg gcaatacatg aagataaaga gtggcataaa
     5281 gcgcttgtca aattgattac catcacgcca gagttagtca ctgacgtcat tcccagggtt
     5341 aatgctaaag cggcaggaat tttatctgaa tgtcgtactc agtcagtcgc tgaagaatgt
     5401 gaatacgcca ctgcggatat gttgccggag ctgtttgtcg ctccaccgtg ggtaataaat
     5461 aagaaaaaga atgtaatacc cgtatttgat ttgccggtac ttcccatccc tgccgtcact
     5521 gatatcacac caggaattac agagttaatc agtcatactg acatatccag attcagtgaa
     5581 atcgcgcaat atcaggcatc acagcaaacg ttatttaccg acctgccacc gataaaaaaa
     5641 gaacgctggg aaacgacttt catcccttta acaccagaac agcaaatcct ttggcgactg
     5701 gggtttaagg aatggcgtag aaccggagaa gaacaatacg aaaaaaaaat aatgccacaa
     5761 agcgcagttg atgccctgtt acgtttcgat ttccccgcgc tgaaagcaga gtttgcgcaa
     5821 taccataaca aaggaagcag acactggcag ttgtacgcgc tctgcttcct acccactcag
     5881 catgctattt cttttcttga tcaaataatt aatgaagagc aattctccgg tgagagagaa
     5941 attctggcta tttttggtga tgcggccatt cctgcgttta tgaaatgtct gcaacgtaag
     6001 ccacaacagt tgtggatatt caccctcttc ctgggcgtca gcgaattagc attaccaatg
     6061 gcgcagcgat tacagaaaaa aatgtctgct gaagatgccc gaaaatggct ggctaacttc
     6121 ccgcgccatg ccgccgccgg gctacttcct gtggccctgg gcaaaaaagg aaaagatcgt
     6181 gattgtgccc gccaggcgct ccgcctgctg gtgaatttaa accaacgaga aacgattgag
     6241 gaaatcgccc gggggtataa ccaaccagat gttttagctg cgctggcaac attgttcgat
     6301 agcgatccac tggaagaata tcctgcaaaa atcgccccat tgcccggctt ttatcagttc
     6361 accttatggc gcagaccacg gcttaaaagt aacaacctgc ctctaccaga tgacgctatg
     6421 cgccacctgg gcactatgct gagctttcct cgcgacatta ccgcctacgc tgggctggat
     6481 atcattagag aaaccttcac ccgcgaatca ctggctgact ttggctggga tctgtatacc
     6541 gcctggacag aagctggcgc acccgcaaaa gaaaactggg catttactgc gcttgggatt
     6601 ttaggcaatg acgacaccgc gcgtaaatta accccgctta tccgtgcctg gcctggggaa
     6661 tcccagcata aacgggcggt gtccgggctg gatgtattag ctgatattgg cagcgatgtt
     6721 gcgctaatgc tgcttaatgg catcgcgcga aaaattaaat tcaaagcatt acaggaacat
     6781 gcccgcgaga aaatcaacat agttgctgaa aaccgtgggc tgactatggc tgaactggaa
     6841 gaccgcctgg ctccagattt agggcttgat agcagtggct cgctgacact tgatttcggc
     6901 ccacgtcaat tcaccgttgg ttttgacgaa accttaaaac ctgtagtgcg cgatgcaaac
     6961 ggcaaagtcc tgaaagattt acccaaacca aatcagagcg atgataaaac actggcaact
     7021 gacgccgtta acctctttaa acaattgaag aaagatgtgc gcgccattgc cagccagcag
     7081 attgatcgtc tggagcaggc tatgtgccag tgcagacgct ggacggcaga gcagttccgc
     7141 ctgtttctgg tggagcatcc gctggtacgt cacttaaccc gacggctgtt atggggggtt
     7201 tataacaatg aaaacgccct tatcacctgt tttcgtgtgg cagaagacag cacttacagc
     7261 gatgcgcagg atgagctatt cacgctgcca gcaggaaaca tcggtattcc gcatgtgctg
     7321 gaaatctccc ctgaatccgc cgctgcattc aggcaaatat acattgatta cgaactgctt
     7381 ccccctttcc aacagcttga tcgtggttgt taccaccttg cggataacga gcgtgatacc
     7441 catgaattgt cacgctggga cggacgtctc tgccaggccg gacgcattgt cgggctggaa
     7501 cgcagaggct ggcaacgtct ggaagaaagc ggcagcgtct atgcgatgcg caaatctacc
     7561 ccttatggcg ctctcgaact tgaaacagaa catttttcat taatttatgg cgaaacgggg
     7621 tatagcgacc tgctacctgt tgaaagcgta aaaatcactt caccagggga acgctacagt
     7681 acacagccct cgctcacgtt ctccgcgctt gatgccatca ccgccagcga actcattaac
     7741 gacatcgaat cactttttga ttaaaaggac tttgcaatga aaacctatat ttatcaggac
     7801 gaaaaatcac ataaattctg ggcggtggag cagcagggaa acgaactgaa tatcagttgg
     7861 ggcaaagtcg gcaccaacgg gcaaagtcag gtaaaaagct ttgcagatgc tgcggcggcg
     7921 gaaaaagcgg aactcaagct gattgcggag aaaacgaaaa aggggtatgt ggaacaagcg
     7981 gagaatgctc catcgaaaca tactcgaacg gtaacgaaat ctctcgaatt atctgacttt
     8041 tctaccatca ttcaggaaca gccctcagtt acaccagaaa tctacgtggc tgacaaaaat
     8101 acagacgtta ttttaccgtg gctggcaaaa gatattgctg tcgtttttcc gcttgaagtc
     8161 gtacccacca ccttaagcca tcgccgcttt cccggagatc ctgttacacc tgtagataaa
     8221 ttaacccagt tacgtcgctt agcctgtggc gtgtcgcaac gggataacaa aacagctaca
     8281 tttgacttca gcgtctgtac tttagagtgg caaaacttca tcgctcaggc gatcgatctg
     8341 attaacagcc cgaaaacgac aaaattacca tcaccggtaa tggctgtact cacggcactt
     8401 gaactgcact gcacgagata taaagagcgt gaggatgtta tggatcagat tattcaggaa
     8461 ggcggtctgg aatatgctac tgatgtaata atacatcttc aacaaatatc tattgaatgg
     8521 gattatgtca acaataatat tgttttcctg tcgtctggca tttcacctga ttatttgcag
     8581 caatattcca gctttgaatt acgcctacgt aaacatttat cactggcgga agagtctctc
     8641 tggcaaaaat gtgcacaaaa acttattgcc gcagttccac atattccaga atggcggcaa
     8701 ccattaattg ctttgttatt acccgaaaaa ccagaaattg cacatgaaat tgcccagcgt
     8761 ctactggggc gaaagaaatt accctcgctt gagtggctaa aaatagtggc gactgatgag
     8821 cacattcttg cctcattaga aaaatatcac gagccatatg ccatttttga tgattactat
     8881 tgtggtgcga tatggtcagc caccgtatta caggagcaag gtgttgcagc cctgccccga
     8941 tttgctccct atgccgcaag tgactactgc gccgatgtgt tgcgtcatat caatcatccg
     9001 ttcgcattaa cactgcttat acgtgtagcc gggcatacta aacgctgtca cgatcgaatg
     9061 acgaaagcct gcgctgcgtt cccacacgca gcactggcag cattggcgga acttctagtg
     9121 caaaaagaag agaatagttg gcgcattatg ctaatgacga tgcttatctc gcaaccgaca
     9181 ctagcagaac aggtcattcc ctggctttcg acacccgcag ttgcagtgct gaaatcatgc
     9241 cagcaacaac tgacacagcc ctcaaaccat gccagcgccg atctactgcc ggccatagta
     9301 gtctccccac catggctttc gaaaaagaaa aaatcgccga ttccggtgct ggatttagca
     9361 ccgctcaacc tcgaatccat atgcacaata accgacacag aggctaaaga gtttcaaact
     9421 cattgggact gggaaccgca taagccaggt gaaggtgcta aaaatttcct ttactcgctg
     9481 gggtatcgcc gctgggattt tgacacatat aaatatatcg gcgcttctga ctcggcaatt
     9541 gacgcatggg aacgtgagga cttcgctaca ctcatccaaa tgtttaaagc ccaccatgca
     9601 ccgtatcagg gggagtggca tctcaactca cttccctttt tacctatgca aaaagcgatt
     9661 aagctatggg aatttctcag caaagagccg cataccgcga taaaaccagt catgctttat
     9721 ttgcgactgg caggtatgag tggttttcta cactcgtttt cacgctaccc tcaagaaggt
     9781 tttgcggttg ctaattattt tgccgcaacg gaactggcac ctgccgtcgc ccgcgccttc
     9841 aacaaactca aaacattacg acaagacgcc agtagctggc tgctgaaata cccgcaacac
     9901 gccattaccg gcctgctgcc tgcagcgctc ggcaaagccg gtgaagcaca ggataacgcc
     9961 cgcgctgcct tgcgtatgct caccgaaaac ggtcatcagc cattgctgca agaaatcgcc
    10021 cggcgttata accagccgga agtaacagac gcgatgaacg ctctgcttgc gctcgatccc
    10081 ttagataatc acccgacaaa aatccccact cttccgacct tttatcagcc atcgctctgg
    10141 acgcgcccgt tattaaaagc aaacgcccaa tcactgcctg atagcgcact cctccacctc
    10201 ggtgaaatgc tccgcttccc tcaggaagag gcactgtatc caggattatt gcaggtgaaa
    10261 gatgcctgta ccaccgactc actggctgaa tttgcctggg atctgtttac cgcctggcag
    10321 accgctggcg cgccgtcgaa agagagttgg gcgttcactg cgttaggcgt tctcggtaac
    10381 gatgacaccg cccgcaaact gacgccatta atccgtgcct ggcctggcga atctcagcat
    10441 aaacgtgcca ccgttgggtt ggatatcctc gccgctatcg gtagtgatat cgccctgatg
    10501 cagcttaacg gcatcgccca gaaactgaaa ttcaaagcat tacaggagcg ggcgaaagag
    10561 aaaattgccg acattgccga aagtcgcgaa ctgacggtag cggagcttga agatcggtta
    10621 gcaccggatc tcggtctgga tgataacggt tcgctgctgc tggattttgg cccacgccag
    10681 ttcaccgtca gctttgatga aaccttaaaa ccgtttgtgc gcgatgtttc cggcagccgc
    10741 ctgaaagact tacctaaacc caataaaagc gatgatgaat cacaggcgaa cgatgcggtt
    10801 aaccgctaca aattgctgaa aaaagatgcg cgtaccgtcg ccgcccagca ggtagcaagg
    10861 ctggaatccg ccatgtgcct gcgtcgccgc tggtcaccgg aaaactttca gctcttcctg
    10921 gttgagcatc cgctggtccg ccacttaacc cgccgtctga tttggggcgt ttatagcgcc
    10981 gaaaaccagc tactggcttg ctttcgcgta gcggaagata acagctacag caccgctgac
    11041 gatgatcttt tcaccctgcc ggaaggcgat atctctattg gtattcctca cgttctggaa
    11101 atatcaccga cggatgctgc cgccttcggg cagctttttg ccgactacga actgttacca
    11161 ccgttccgcc agcttgatcg taacagttac gcgctgacag aagccgagcg caatgccagc
    11221 gaactgacac gctgggcagg cagaaaatgc ccgagtggtc gggtaatggg gctggcgaat
    11281 aaaggctgga taaagggcac cccgcaggat gctggctgga tcggctggat gatcaacccc
    11341 ctgggccgct ggtcgttaat catggaaatc gatgaaggtt ttgcggtagg catgtcgcca
    11401 gccgaactga gtgccgagca aatcttaagc aagctgtggc tatgggaagg caaagcagaa
    11461 agctatggct gggggagtaa ttcaacacag gaagcgcagt tctccgtact cgataccatc
    11521 accgccagcg agctaattaa cgatattgaa gccctgtttg aataaggaaa gagcatggac
    11581 aaggaattac cgtggctggc ggataacgcc caactggaac ttaaatataa aaaaggcaaa
    11641 acaccactca gccatcgcaa ttggccaggc gaaccggtac ctgttatcac tgaaaacatc
    11701 attcagacac tgggtgatga attgctacaa aaagcagaga agaaaaaaaa cattgtctgg
    11761 cgttatgaaa acttttcact ggagtggcag tccgccatca cgcaggccat caacttgatc
    11821 ggcgaacaca aaccctcaat cacggcccgg acaatggcga cgctagcctg tatcgcgcaa
    11881 aatgatagcc aacagttgct cgacgaaatc gtccaacaag aggggctgga atatgcaact
    11941 gaggtggtga ttgcacgcca gtttattgta cggtgttatg agagtgatcc tctggtagta
    12001 acattgcaat atcagaacga ggactatggc tatggttatc gctcagaaac ctataacgaa
    12061 ttcgatctcc gattgcgtaa gcatctctct ctggcagagg aaagctgctg gcagcgttgc
    12121 gccgacaaac tcattgccgc actgccagga ataaccaaag ttcgccgccc ttttattgcg
    12181 ctgaacctcc cggaaaaacc cgaaatcgcc aacgagctgg taagccttga atgttcgcaa
    12241 actcattttc gctcaaaaga gtggttaaaa gttgttgctg atgactctaa ggcggtgaaa
    12301 gaactcgcac gttactggag ccaggatata tttagcgatc gagaagccag ctacatgtcg
    12361 catgaaaacc acttcggtta cgcggcctgc gctgcccttt tgcgcgaaca aggactggca
    12421 gccgttccgc gcctcgcgat gtatgcacat aaagaagatt gcggcagtct gctggtacaa
    12481 attaaccatc cgcaagtcat ccgcaccttg ctactggtgg ctgataaaaa caaacccagc
    12541 ctgcaacgtg tggctaaata cagtaaaaac ttcccccatg cgacgctcgc cgcactggca
    12601 gaactgctgg cgttaaaagc cccccctgcg cgccctggtt atccaatcat cgaagacaaa
    12661 aagctgcctg cacagcaaaa agcacgtgat gaatactggc atacgctgtt acaaacgctg
    12721 atggcatcgc agccacaact ggcagaagag gtgatgccgt ggttaagtac tcaagcccag
    12781 gcagtggtga ataattattt atcgacatcg ccaaaaccgg ttattgatgg caccgataac
    12841 agccaaatgc ctgaaatcct cgtttcacca ccgtggcgta gtaagaaaaa aatgacagct
    12901 ccacgtcttg atttggcacc gctcgaatta actccgcaag tttactggca accaggcgaa
    12961 cgagagagac ttgccgccac agagtctgcc cgttatttca gcacgggatc ttttacggaa
    13021 cgcatggaac gaaaaagtgg tcgagttgta ctacaggaac tgggttttgg ggatgatgta
    13081 tggctgtttt tgaattatat actccccgga aaactggatg ctgcacgcaa ttcactcatt
    13141 gttcagtggc attactacca ggggcgagtt gaagagatcc tgcatggctg gaactccccg
    13201 gaagcacaat tagcagaaca ggcgcttcgc agcggtcacg tagaagcgtt aattaacata
    13261 tgggaaaatg acaactactc acgttatcgt ccggaaaaga gtgtctggaa cctgtattta
    13321 ttggcacagt tgccgcgtga gatggctttg actttctggc tgcgtatcaa tgagaaaaag
    13381 catctgttcg cgggtgagga ctattttctc agtatcctcg ggttggatac attacctggc
    13441 ctgttattgg ctttttcaca tcatccaaaa gaaacatttc cgttaatttt aaatttcggc
    13501 gcaacagaac tggccctgcc cgttgcccgc gtctggcgac gtttcgcggc gcagcgtgat
    13561 ctggctcgcc agtggatttt acaatggccg gaacatacgg ctactgcact tatccctctt
    13621 gtctttacaa aacccagcga taatagcgaa gctgcattac ttgccctgcg tttactgtat
    13681 gaacaagggc atggcgaatt actgcaaacc gtggcaaacc gctggcagcg tacagatgta
    13741 tggcctgccc tggagcaatt acttaaactg ggtccaatag aaatttaccc ggcacgtatt
    13801 ccaaaagctc ctgatttctg gcatccggca atgtggtcca ggccgcgcct tatcaagaat
    13861 aatcagcctg ttaccgatga cgctctggaa attatcggcg aaatgctgcg ctttacccag
    13921 ggcggacgtt tttatagcgg actggaacag ctgaaaacgt tttgccagcc gcaaacgctg
    13981 gcagcttttg cctgggatct tttcaccgcg tggcaacaag ctggtgcctc cgcaaaagac
    14041 aactgggcat ttctggcgtt aagtctcttt ggtgacgaaa gcacggctcg ggatctgacg
    14101 acacagatcc tcgcctggcc acaagaaggc aaatctgccc gtgctgtcag tggcctgaac
    14161 atccttaccc tgatgaataa tgatatggcg ctgatacagc tgcaccatat atcccaacgg
    14221 gcgaaatccc gctccttacg tgataacgcg gcggaatttc ttcaggtggt cgcagaaaat
    14281 cgcgggctaa gccaggaaga gttagcggac agattagtcc caacattggg ccttgatgat
    14341 ccgcaggcgt tgagttttga ttttggcctc cggcagttta ccgttcgctt tgatgaaaac
    14401 ctcaacccgg ttatctacga tcaacaaaac gttcgccaga aaagtgttcc ccggttgcgc
    14461 gccgatgacg atcaactgaa agcgcccgag gcactggccc gactaaaagg gctaaaaaaa
    14521 gatgccactc aggtgagcaa aaacctgctc ccacgtcttg aagcagccct acgtatcacc
    14581 cgccgctggt cgcttgcaga ttttcattct ctgtttgtta atcatccctt tacccgcctg
    14641 gttacccagc gattaatatg gggggtttat ccggcaaatg aaccgcgtcg tttactcaac
    14701 gcctttcgtg tggccgcaga gggggagttc tgcaatgagc aagatgagcc aattgacctg
    14761 cctgccgatg ctctgattgg cattgcccac ccgttagaaa tggcagcaga aatgcgcagt
    14821 gaatttgcac agctttttgc cgattacgaa attatgccgc cttttcgcca gttggcgcgg
    14881 cgcacggtgc tgctcacacc tgacgagtca gccagtaaca gcctgactcg ctgggaaggt
    14941 aaatccgcta ccgttgggca acttatggga atgcgataca aaggctggga gtcaggctat
    15001 gaggacgcat ttgtctatga cttaggtgcg taccggctgg tccttaagtt ttcacccggt
    15061 tttaaccact acaatgttga tagcaaagcg ctaatgagct tccattctct tcgggtttac
    15121 cgtgacaata aatccgtcac ttttgccgaa cttgatgtgt ttgatttgag tgaagcgtta
    15181 agcgcacccg acgttatttt ccattaaaac acagcgggca gtgatgcaac tgcccgttat
    15241 cgacacgact tacctccggg aaaagcgatg atcgtacaaa aagagctggt cgctatttac
    15301 gattatgaga ttcctgctcc tgaagatccg ttttccttca gacttgagat ccataaattc
    15361 tctgaattat ttacaggttc cgtctatcga ctggaacgat tccggctaca tccaacattt
    15421 aatgatgccg actcgctaat aaatgatgcg ttgatttata taagagatga ttttattgat
    15481 gagcggaaat tacgaggtga atcacatgaa acagtaatag caatttttaa ttgtgaacta
//
LOCUS       DADSYM010000119        15440 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2415
            SAMN08039579-rid11723173.denovo.120, whole genome shotgun sequence.
ACCESSION   DADSYM010000119 DADSYM010000000
VERSION     DADSYM010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039579
            Sequence Read Archive: SRR6362533
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15440)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15440)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980162.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 03:39:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,484
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 4,347
            CDSs (with protein)               :: 4,347
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 43 of 85
            Pseudo Genes (incomplete)         :: 37 of 85
            Pseudo Genes (internal stop)      :: 19 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15440
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039579-rid11723173.denovo.120"
                     /strain="LGE2415"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8302 N 95.9628 W"
                     /collection_date="2015-08-24"
     gene            69..1460
                     /gene="xanP"
                     /locus_tag="J1Y83_003250"
     CDS             69..1460
                     /gene="xanP"
                     /locus_tag="J1Y83_003250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine/proton symporter XanP"
                     /protein_id="HBA7631371.1"
                     /translation="MSVSTLESENAQPVAQTQNSELIYRLEDRPPLPQTLFAACQHLL
                     AMFVAVITPALLICQALGLPAQDTQHIISMSLFASGVASIIQIKAWGPVGSGLLSIQG
                     TSFNFVAPLIMGGTALKTGGADVPTMMAALFGTLMLASCTEMVISRVLHLARRIITPL
                     VSGVVVMIIGLSLIQVGLTSIGGGYAAMSDNTFGAPKNLLLAGVVLALIILLNRQRNP
                     YLRVASLVIAMAAGYALAWFMGMLPESNEPMTQELIMVPTPLYYGLGIEWSLLLPLML
                     VFMITSLETIGDITATSDVSEQPVSGPLYMKRLKGGVLANGLNSFVSAVFNTFPNSCF
                     GQNNGVIQLTGVASRYVGFVVALMLIVLGLFPAVSGFVQHIPEPVLGGATLVMFGTIA
                     ASGVRIVSREPLNRRAILIIALSLAVGLGVSQQPLILQFAPEWLKNLLSSGIAAGGIT
                     AIVLNLIFPPEKQ"
     gene            1581..3290
                     /locus_tag="J1Y83_003251"
     CDS             1581..3290
                     /locus_tag="J1Y83_003251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HBA7631372.1"
                     /translation="MKFIGKLLLYILIALLVVIAGLYFLLQTRWGAEHISAWVSENSD
                     YHLAFGAMDHRFSAPSHIVLENVTFGRDGQPATLVAKSVDIALSSRQLTEPRHVDTIL
                     LENGTLNLTDQTAPLPFKADRLQLRDMAFNSPNSEWKLSAQRVNGGVVPWSPEAGKVL
                     GTKAQIQFSAGSLSLNDVPATNVLIEGSIDNDRVTLTNLGADIARGTLTGNAQRNADG
                     SWQVENLRMADIRLQSEKSLTDFFAPLRSVPSLQIGRLEVIDARLQGPDWAVADLDLS
                     LRNMTFSKDDWQTQEGKLSMNASEFIYGSLHLFDPIINAEFSPQGVALRQFTSRWEGG
                     MVRTSGNWQRDGKTLILDDAAIAGLEYTLPKNWQQLWMETTPGWLNSLQLKRFSASRN
                     LIIDIDPDFPWQVTALDGYGANLTLVTDHKWGVWSGSANLNAAAATFNRVDVRRPSLA
                     LTANSSTVNISELSAFTEKGILEATASVSQTPQRQTHISLNGRGVPVNILQHWGWPEL
                     PLTGDGNIQLTASGDIQVNVPLKPTVSGQLHAVNAAKQQVTQTMNAGVVSSGEVTSTE
                     PVQ"
     gene            complement(3334..4014)
                     /locus_tag="J1Y83_003252"
     CDS             complement(3334..4014)
                     /locus_tag="J1Y83_003252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016235022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lyxose/D-mannose family sugar isomerase"
                     /protein_id="HBA7631373.1"
                     /translation="MKRSQINYVIDKAHAIAQTFRVCLPEFAYFTVDDWLQRERDNWQ
                     EVVDLQLGWDITDFGRGDFNQTGLTLLTMRNGALGSAAYPKPYAEKMLQIQQDQQTPW
                     HFHTHKMEDILNRGGGDLCMQLAWANEANLCDDQRVITVSVDGQRRTMKPGETLVLKP
                     GQGICLPPRLYHRFWAEKAFVLGWEISMVNDDQHDNYFLEPGGRFPAIEEDESVKWLL
                     CSEYGILR"
     gene            complement(3995..4927)
                     /locus_tag="J1Y83_003253"
     CDS             complement(3995..4927)
                     /locus_tag="J1Y83_003253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016238590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HBA7631374.1"
                     /translation="MHYLGLDIGGTKIAAVVMDAHGWEIRRYRCPTQKSTYQQFVSCV
                     VALIEQIRRDVQRPMLTGIALPGSISPLTGLIKNANIQVINGHALQADLQQLLGQPVV
                     IANDGNCFALSEACDGAGQDYDVVFGITLGTGCGGGIAIKQRPFIGAWGNAAECGHIT
                     LPGYTEQEDGPSVSCYCGKHNCVESFVSGSGFSERYQQMTGNLLTPAAIVTLAQRGDA
                     CAMQQVARFRQQLARTLATIVNVVDPGVIVIGGGLSNVELLITDLNAEVAPLVFTDQF
                     TTPIVKAQHGDSSGMRGAAWLAMRNGEANETFTN"
     gene            complement(4975..5835)
                     /locus_tag="J1Y83_003254"
     CDS             complement(4975..5835)
                     /locus_tag="J1Y83_003254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006687642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HBA7631375.1"
                     /translation="MLVSMKELLQPTRQHGFAIGAFNVADDCFLRAVVDEAEATNTPA
                     IIAIHPSEHDFVGDAFFAHVREITQRSPVPFVLHLDHGASVEHVLRAIRCGFTSVMID
                     GSLLPYEENVALTAEVVRLAHAVGVSVEGELGTIGQTGTSVEGGVSQVTYTDPAQAAD
                     FIARTGADTLAVAIGTAHGIYPKGMQPKLQMDILRDIAGRIDIPLVLHGGSANPDAEI
                     AESVTLGVGKINISSDMKYAYFQKVREILAKESWWDPNVIYPDAINAAREVILHKMKL
                     FGSLGKASLY"
     gene            complement(5916..6767)
                     /locus_tag="J1Y83_003255"
     CDS             complement(5916..6767)
                     /locus_tag="J1Y83_003255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007370550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase family
                     protein"
                     /protein_id="HBA7631376.1"
                     /translation="MFADMKSMVMKAWHEHYALLAINCMNLESAHAAIRVAEKNRAPI
                     ILNLYQGHLAHFPAPVAAAVVKTLAESASVPVTLALDHGKNPDCIRQAFRAGFSGLMI
                     DASAFPLEENVRQTRAVVELAASAQLCVEGELGHLADAPRYDQAANADLMTQPTDVEP
                     FIAQTGIDLLAVSVGTAHGMYAPGVVPAIDFQRLAEIFHCSSVPLALHGGSGTPFDQL
                     QLCTTLGVAKINVGAAIFERGKSALLHTLCHDISIELVDALKAMELAFEEAITPYLQA
                     SGAIGKA"
     gene            complement(6779..7870)
                     /locus_tag="J1Y83_003256"
     CDS             complement(6779..7870)
                     /locus_tag="J1Y83_003256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009112672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HBA7631377.1"
                     /translation="MKDNKIPVSQEIKKHLLTGISWMIPLIVAAGICIALGQVLGGTD
                     VGEKTGTIPWMLNQIGGWGMGLIVPLISAAIAYSIADRPGFAPGLIVGFLCGQIHTGF
                     IGGMLGGFLVGYTILLLKRYIRLPQSMQGLMPIMVLPVLSTIIGGLLMMTLIGKPIAW
                     LQEALIHLLESMQGGSRFLMGAILGAMATFDFGGPVNKTMSLFSDGLLVSGVYGPEAV
                     KFVGSIIPPFGITLSFLLTRHKYTRAEREALKAAFPMGICMITEGVIPIAARDLLRVV
                     GSCVVASAVAGGLIMVWGVESPVPHGGMFVVPLFTHPLLFCLALGIGTVICGVMLSLW
                     KKPVTERDEEFDELSDQKLKDEEITFTLE"
     gene            complement(7895..8209)
                     /locus_tag="J1Y83_003257"
     CDS             complement(7895..8209)
                     /locus_tag="J1Y83_003257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001028073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HBA7631378.1"
                     /translation="MNIVCVAACTAGIAHTYIAREKLIKGAKALGHTIKVETQGTIGT
                     ENELQAADISAADVVILAVDVKIKGEERFTNKRIVRVKTEIVIKSPVQFLEKVEKSLG
                     NK"
     gene            complement(8227..8697)
                     /locus_tag="J1Y83_003258"
     CDS             complement(8227..8697)
                     /locus_tag="J1Y83_003258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017801221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIA"
                     /protein_id="HBA7631379.1"
                     /translation="MDITKILNTNRVILDMQATNKAEAIEELTNLLKKDGAIDCRETF
                     IKDVWQREAEGSTGFENHIAIPHGKSSAVINTTLAIGRTRQDIPWETLDGSSVRCIIL
                     FAVRLEDQNTTHIRLLSQVAGALADDDIIEQLLVETSPQKIIDLFSQYAQSDVC"
     gene            complement(8724..10277)
                     /locus_tag="J1Y83_003259"
     CDS             complement(8724..10277)
                     /locus_tag="J1Y83_003259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016237594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HBA7631380.1"
                     /translation="MQMITSRQNRLLRFLLPRREYTTIVTIAGYLNVSEKTIQRDLRL
                     LEQWLGQWRINVEKRAGAGVMLSAENIADLLHLDHLLGAECEEIDGVMNNARRVKIAS
                     QLLSETPNETSISKLSERYFISGASIVNDLRVIESWLAPLGLSLIRSPSGTHIEGSEG
                     QVRQAMALLINGIINHNEPQGVVYSRLDPGSYKALVHYFGEEEVLFVQSLLLDMENEL
                     SWSLGEPYYVNIFTHILIMMYRNTHGNALSREEDQTRQYDENIFNVASQMIHKIEQRI
                     AHTLPDDEVWFIYQYIISSGVAIDGQKDVSIISHMQASNEARLITWRLITVFSDIVDC
                     DFSEDSALYDGLLVHIKPLINRLNYRIHIRNPLLEDIKAELADVWRLTQYVVNQVFKT
                     WGENAVSEDEVGYLTVHFQAAMERQIARKRVLLVCSTGIGTSHLLKSRILRAFPEWTI
                     VDVISAANLSQVLPDNIELIISTINLPTVTMPVAYVTAFFNDADIKRVTEMVITEKLH
                     HATSRVVEI"
     gene            complement(10572..12890)
                     /gene="yicI"
                     /locus_tag="J1Y83_003260"
     CDS             complement(10572..12890)
                     /gene="yicI"
                     /locus_tag="J1Y83_003260"
                     /EC_number="3.2.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-xylosidase"
                     /protein_id="HBA7631381.1"
                     /translation="MKISDGNWLIQPGLNLIHPLQVFEVEQQGNEMVVYAAPRDVRER
                     TWQLDTPLFTLRFFSPQEGIVGVRIEHFQGALNNGPHYPLNILQDVKVTIENTERYAE
                     FKSGNLSARVSKGEFWSLDFLRNGERITGSQVKNNGYVQDTNNQRNYMFERLDLGVGE
                     TVYGLGERFTALVRNGQTVETWNRDGGTSTEQAYKNIPFYMTNRGYGVLVNHPQCVSF
                     EVGSEKVSKVQFSVESEYLEYFVIDGPTPKAVLDRYTRFTGRPALPPAWSFGLWLTTS
                     FTTNYDEATVNSFIDGMAERNLPLHVFHFDCFWMKAFQWCDFEWDPLTFPDPEGMIRR
                     LKAKGLKICVWINPYIGQKSPVFKELQEKGYLLKRPDGSLWQWDKWQPGLAIYDFTNP
                     DACKWYADKLKGLVAMGVDCFKTDFGERIPTDVQWFDGSDPQKMHNHYAYIYNELVWN
                     VLKDTVGEEEAVLFARSASVGAQKFPVHWGGDCYANYESMAESLRGGLSIGLSGFGFW
                     SHDIGGFENTAPAHVYKRWCAFGLLSSHSRLHGSKSYRVPWAYDDESCDVVRFFTQLK
                     CRMMPYLYREAARANARGTPMMRAMMMEFPDDPACDYLDRQYMLGDNVMVAPVFTEAG
                     DVQFYLPEGRWTHLWHNDELDGSRWHKQQHSFLSLPVYVRDSALLALGNNDQRPDYAW
                     HEGTAFHLFNLQDGHEAVCEVPDADGSVIFTLKAARTGNTITVIGTGEAKNWTLCLRN
                     IVKVNGLQGGSQAESEQGLVVTPQGNALTITL"
     gene            complement(12900..14282)
                     /locus_tag="J1Y83_003261"
     CDS             complement(12900..14282)
                     /locus_tag="J1Y83_003261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418114.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside-pentoside-hexuronide family
                     transporter"
                     /protein_id="HBA7631382.1"
                     /translation="MKSEVLSVKEKIGYGMGDAASHIIFDNVMLYMMFFYTDIFGIPA
                     GFVGTMFLVARALDAISDPCMGLLADRTRSRWGKFRPWVLFGALPFGIVCVLAYSTPD
                     LSMNGKMIYAAITYTLLTLLYTIVNIPYCALGGVITNDPTQRISLQSWRFVLATAGGM
                     LSTVLMMPLVNLIGGDNKPLGFQGGIAVLSVVAFMMLAFCFFTTKERVEAPPTTTSMR
                     DDLRDIWQNDQWRIVGFLTIFNILAVCVRGGAMMYYVTWILGTPEVFVAFLTTYCVGN
                     LIGSALAKPLTDWKCKVTIFWWTNALLAVISLAMFFVPMQASITMFVFIFVIGVLHQL
                     VTPIQWVMMSDTVDYGEWCNGKRLTGISFAGTLFVLKLGLAFGGALIGWMLAYGGYDA
                     AEKAQNSVTISIIIALFTIVPAICYLLSAIIAKRYYSLTTHNLKTVMEQLAQGKRRCQ
                     QQFTSQEVQN"
     gene            14575..14669
                     /locus_tag="J1Y83_003262"
     tRNA            14575..14669
                     /locus_tag="J1Y83_003262"
                     /product="tRNA-Sec"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:14609..14611,aa:Sec,seq:tca)
     gene            14978..15285
                     /locus_tag="J1Y83_003263"
                     /pseudo
     CDS             14978..15285
                     /locus_tag="J1Y83_003263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611997.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT         3941 a         4077 c         3626 g         3796 t
ORIGIN      
        1 tggcaaacgt ttgcttttct ctttcggtca gctaaaatgc ccgctttgct accacgggat
       61 tgttttcgat gtctgtttcc accctcgagt cagaaaatgc gcaaccggtt gcgcagactc
      121 aaaacagcga actgatttac cgtcttgaag atcgtccgcc gcttcctcaa accctgtttg
      181 ccgcctgtca gcatttgctg gcgatgttcg ttgcggtgat cacgccagcg ctattaatct
      241 gccaggcgct gggtttaccg gcacaagaca cgcaacacat tattagtatg tcgctgtttg
      301 cctccggtgt ggcatcgatt attcaaatta aggcctgggg tccggttggc tccgggttgt
      361 tgtctattca gggcaccagc ttcaactttg ttgctccgct gattatgggc ggcacagcgc
      421 tgaagaccgg tggtgctgat gttcctacca tgatggcagc tttgttcggc acgttgatgc
      481 tggcaagttg taccgagatg gtgatctccc gcgttctgca tctggcgcgc cgcattatta
      541 cgccgctggt ttctggcgtt gtggtgatga ttatcggcct gtcgctaatt caggttgggc
      601 tgacctccat tggcggcggt tacgcagcca tgagcgataa caccttcggc gcaccgaaaa
      661 atctgctgct ggcaggcgtg gtcttagcct taattatcct gcttaaccgt caacgtaacc
      721 cttacttacg cgtggcctca ctggtgattg cgatggcggc cggatatgcg ctggcgtggt
      781 ttatgggcat gttgccagaa agcaacgaac cgatgacaca agaactgatt atggtgccaa
      841 cgccgctcta ttatggtctt ggcattgaat ggagtctgct gctgccgctg atgctggtct
      901 ttatgatcac ttcgctggaa accattggcg atatcacggc gacctctgac gtttccgaac
      961 agccagtttc cggtccgctg tacatgaaac gtctgaaagg cggcgtgctg gcaaacggcc
     1021 tgaactcgtt tgtctcggcg gtatttaata ccttcccgaa ctcctgcttc gggcagaaca
     1081 acggggtgat ccagttaacc ggtgttgcca gccgctatgt cggttttgtc gtcgcgctga
     1141 tgttgatcgt gctgggtctg ttcccggcag tgagcggttt tgtacaacac attccagaac
     1201 cggttctggg cggcgcaacg cttgtaatgt ttggcaccat cgccgcctcc ggtgtgcgta
     1261 tcgtttctcg tgagccgctg aaccgccggg cgattctgat tatcgcgctg tcgctggcgg
     1321 tcggtctggg cgtgtctcag cagccgctga ttttgcagtt tgctcctgaa tggctgaaaa
     1381 acctgctctc ctccgggatt gccgcgggcg gtattactgc catcgtgctg aatctgattt
     1441 tcccaccaga aaaacagtaa ttcactggcg gcggtaagat aattaccgcc gcagtctcac
     1501 ctctttcacc attcccgcct tgaggattgc acgtaaatcg tgcataactc ccttatgtgc
     1561 atttcacggg atggaagacc atgaaattta ttgggaagct gcttctctac attctcatcg
     1621 ctctgttagt ggtgatcgct ggcctctatt ttcttctgca aactcgctgg ggagcagaac
     1681 atatcagcgc atgggtttcc gagaatagcg actatcatct ggccttcggg gcgatggatc
     1741 accgtttttc cgcgccatct catatcgtgc tggagaacgt cacgtttggc cgtgatggcc
     1801 agcccgcgac cctggtagca aaaagtgtcg acattgcgct aagcagtcgg caactgaccg
     1861 aaccacgcca tgtcgatacc atcctgctgg aaaacgggac gctgaatctc accgaccaga
     1921 ccgcgccgct accgttcaaa gccgatcgtc tgcaactgcg tgatatggcg tttaatagcc
     1981 cgaatagcga atggaaactg agcgcgcagc gggtaaatgg cggcgtggtt ccgtggtcac
     2041 cagaagccgg taaagtgctg ggtacgaagg cacagattca gtttagtgcc ggatcgcttt
     2101 cgctcaatga tgttcctgcc accaatgtac tgattgaagg cagtattgat aacgatcgcg
     2161 ttacgctgac taacctgggt gccgacatcg cccgcgggac attaaccgga aacgcgcagc
     2221 gtaacgctga cggcagctgg caagtggaaa acctgcgcat ggcggatatc cgtctacaaa
     2281 gcgaaaaatc gctaaccgac ttctttgcac cattacgctc tgtcccgtcg ttgcagattg
     2341 gtcgcctgga agtcatcgat gctcgtttgc aaggtccgga ctgggcggtg gccgacctcg
     2401 atctcagctt gcgcaatatg accttcagta aagatgactg gcagacacag gaaggtaaac
     2461 tgtcgatgaa cgccagcgag tttatttatg gctcgctgca tttatttgat ccgattataa
     2521 acgcggaatt ttccccgcag ggcgtagcac tgcgccagtt caccagccgc tgggaagggg
     2581 gtatggtcag aacgtcagga aactggcaac gcgacgggaa aacgctgatc ctcgatgatg
     2641 cggcaattgc cggactggaa tataccttgc ccaaaaactg gcaacagttg tggatggaaa
     2701 cgacacccgg ttggttaaac agcctgcaac tgaagagatt tagcgccagc cgcaatctga
     2761 tcattgatat cgaccctgac ttcccgtggc aggtcaccgc gctcgatggt tacggtgcca
     2821 atctgacact ggttaccgat cataaatggg gcgtctggag tggctcggcg aatctgaatg
     2881 ccgccgccgc aacgtttaat cgtgttgatg tccgtcgtcc gtcgctggcg ctaactgcca
     2941 acagcagcac ggtgaatatc agcgaactga gtgcatttac tgaaaaaggc attctggaag
     3001 ccaccgccag tgtttcacaa acgccacaac gtcagaccca tatcagcctg aatggacgcg
     3061 gtgtgccggt gaatattttg caacattggg gatggcctga attaccgttg actggtgacg
     3121 gcaatattca gcttaccgcc agtggcgata ttcaggtcaa tgtcccgctg aaacctacgg
     3181 ttagcgggca attgcatgcc gtgaatgccg caaagcagca agtgactcaa accatgaatg
     3241 cgggcgtagt ttccagtggt gaagttacgt cgacggagcc ggtgcagtaa aaataaggac
     3301 gctagcggat atcagcgcta gcgttccccg ttattagcgc aaaattccat actcactgca
     3361 caataaccat ttcaccgatt catcttcttc tatcgcagga aaacgtcctc ccggctcaag
     3421 aaaataatta tcgtgttggt catcatttac catcgagatt tcccagccga gaacaaacgc
     3481 tttttccgcc cagaagcgat gatacagacg cggtggcagg caaatgccct gccccggctt
     3541 tagcaccagc gtttcaccag gtttcatggt acgccgctga ccatcaacac tcacggtaat
     3601 gactcgttga tcatcacaga gattggcttc gttggcccag gccaactgca tgcataagtc
     3661 tccaccgccc cgattgagga tatcttccat tttgtgggtg tgaaaatgcc agggagtctg
     3721 ctgatcttgt tggatctgca acattttctc agcatacggt tttggatatg ccgccgaccc
     3781 cagcgcgccg ttgcgcatcg ttagcagtgt tagtcccgtt tggttgaaat caccacgacc
     3841 aaaatcggtg atatcccaac cgagctgtaa gtcgaccact tcttgccagt tatcccgttc
     3901 ccgctgcagc cagtcatcca ccgtaaaata ggcgaactct ggcaaacaaa cgcggaaggt
     3961 ctgcgcaatg gcatgtgctt tatcaataac ataattaatt tgtgaacgtt tcattggctt
     4021 ctccgttgcg catagcaagc caggcagcgc cacgcattcc gctactgtcg ccgtgctgtg
     4081 cttttacaat gggagtggtg aattgatcgg tgaaaaccag tggagcgact tctgcgttca
     4141 gatcggtgat aagcagttcc acattcgaaa gcccgccgcc gatcacaatc acgccagggt
     4201 caacaacgtt aacgatggtt gccagcgtgc gggcaagctg ttggcgaaaa cgtgccacct
     4261 gctgcatggc acaagcatca ccacgttgtg ccagggtgac aatcgccgca ggagtgagca
     4321 agttaccagt catctgttga tagcgttcac taaaaccgct gccggaaaca aacgactcca
     4381 cgcagttgtg tttgccgcaa tagcaactga ctgatggacc atcttcctgc tccgtatagc
     4441 ctggcaacgt gatatgaccg cattcggcag catttcccca ggcccctata aacggtcgtt
     4501 gcttgatggc aatgccaccg ccgcagcccg taccaagcgt aataccaaat accacgtcat
     4561 aatcttgccc ggcaccgtcg caagcttctg atagcgcgaa acagttacca tcattggcta
     4621 tcaccaccgg ttgcccaagc aattgctgca aatcagcctg taacgcatga ccgttaatca
     4681 cctgaatatt cgcgttttta atcaggccag tgagtggcga gatactgccg ggtaaggcga
     4741 tccctgtcag catcggtcgt tgaacgtccc gcctaatctg ctcgataagc gccacaacgc
     4801 atgagacaaa ttgttgatat gtcgactttt gcgtcgggca gcggtaacgg cgaatctccc
     4861 agccatgcgc atccatgaca acggcggcga ttttggtccc accaatatcc aaccccagat
     4921 agtgcatatc tcgttccttg ttgcgtaaaa ggcgggtcac cccgcccttc tggcttagta
     4981 cagtgatgct ttaccgagcg aaccgaacag tttcattttg tggagaatga cttcccgcgc
     5041 cgcgttaatc gcatccggat agatgacgtt cgggtcccac cagctctctt tagcgagaat
     5101 ttcacgcact ttctggaaat aggcgtattt catatcgctg gagatattga ttttgccgac
     5161 gccgagcgta actgattcag caatctccgc gtccgggttt gccgaaccac cgtgtagcac
     5221 cagcggaata tcaatacgtc cggcaatgtc gcgcaaaatg tccatttgca gcttcggctg
     5281 catccctttt ggatagatgc catgcgctgt accaatcgcc acagccagtg tgtcggcacc
     5341 tgtacgagca ataaaatcgg cggcctgcgc cggatctgtg taggtcactt gtgaaacacc
     5401 accttctacc gacgttcctg tttgtccgat ggttcccagt tcaccctcaa ccgatacgcc
     5461 aaccgcgtga gcgaggcgaa ccacttctgc ggtcagcgcc acgttttctt catacggcag
     5521 cagcgaaccg tcgatcatta ctgaggtaaa tccgcagcga atcgcacgca aaacatgctc
     5581 tacagaggca ccatgatcga ggtgcagtac aaacgggacc ggactacgct gggtaatttc
     5641 gcgaacgtga gcgaaaaagg catcgcccac aaaatcatgt tcgctgggat ggatggcaat
     5701 aatcgccgga gtatttgtcg cttctgcttc atccacgact gcgcgaagga aacagtcatc
     5761 cgccacatta aatgcgccaa tggcaaagcc gtgctggcgg gtaggttgca gtaattcttt
     5821 catcgaaact aacatgttaa atctccagtt tcagcgtgta gtccgccctg ccggtgcgtt
     5881 attgcactgg cataaaggcc atgacagaaa atgaatcagg ctttgccgat agcgccgctc
     5941 gcctgtaaat acggtgtaat cgcttcttcg aaggccagtt ccatagcttt cagcgcgtcc
     6001 accagttcaa tggatatatc gtgacagagg gtatgcagga gcgcggattt cccccgttca
     6061 aaaatggccg cccccacgtt gatttttgct acccccagcg tcgtacagag ctggagctga
     6121 tcgaacggcg taccgctacc gccatgtaat gccagaggca cgctcgagca gtggaaaatc
     6181 tccgccagac gctgaaaatc aatagcaggt acaacaccag gcgcgtacat gccgtgcgcc
     6241 gtgcctaccg agacggccag tagatcaatg cctgtctgcg cgataaacgg ttccacatca
     6301 gtgggttgcg tcatcaggtc tgcattggcg gcctggtcgt aacgcggcgc atctgccaga
     6361 tggcccagtt cgccttccac gcagagctgc gcgctggcgg cgagttccac caccgccctg
     6421 gtctggcgca cgttttcctc caggggaaaa gcagaagcat caatcattaa tccgctgaaa
     6481 ccagcacgaa acgcctgacg aatacagtcc ggatttttgc catgatcaag cgccagagtg
     6541 accggtactg aggcagattc cgcgagcgtt ttaaccactg ccgccgcgac gggtgcagga
     6601 aaatgcgcca gatgcccctg atagagattg agaatgatcg gagcgcgatt cttctccgcc
     6661 acgcggatag ctgcgtgagc actttccaga ttcatacagt tgatcgccag caaggcgtaa
     6721 tgttcatgcc aggctttcat caccatactt ttcatatcgg caaacatagg gcctccggtt
     6781 attcgagggt gaaagtgatc tcttcatctt tcagcttttg atcgctcagt tcatcaaact
     6841 cttcgtcgcg ttccgttacc ggtttcttcc agagtgaaag catcacgcca caaatcaccg
     6901 tgcctatacc cagcgccagg caaaacagca gaggatgggt gaacagaggc acaacaaaca
     6961 tgccgccatg cggaaccgga ctctccacgc cccacaccat gattaagccg cccgcgaccg
     7021 ccgatgccac cacacaagat cccactactc gcagcaggtc acgcgcggcg atcggaatga
     7081 ctccttcagt gatcatgcag atccccatcg gaaacgcggc tttcagcgcc tcgcgctccg
     7141 cacgggtgta tttatggcgt gtcagtaaga acgaaagtgt gatgccaaac ggagggatga
     7201 ttgagccgac aaatttcacg gcttctggtc cgtagacgcc actgaccagc aagccatcgg
     7261 aaaacagcga catggttttg ttgaccggac caccgaaatc gaaggttgcc atcgcgccaa
     7321 gtatggcccc catcaggaag cgcgatccac cctgcatcga ctcgagcaaa tgaatcagtg
     7381 cttcttgtaa ccaggcgatg ggtttaccaa tcagcgtcat cattaacagc ccaccgataa
     7441 tggtgcttaa taccggcaac accatgattg gcatgagtcc ttgcatcgac tgcggcaggc
     7501 ggatatagcg tttaagtagc aaaatggtgt agcccaccag gaaaccaccc aacataccgc
     7561 caataaatcc ggtatggatt tgcccgcaga gaaagccgac aatcaggccc ggggcaaatc
     7621 cgggacgatc ggcaatagaa taggcaattg ccgcgctaat cagcggcaca atcagcccca
     7681 ttccccagcc gccaatctga ttaagcatcc acgggattgt tcctgttttt tcaccaacat
     7741 ctgtaccgcc aagaacttgc cccaacgcaa tacatatccc ggcggccaca ataaggggta
     7801 tcatccacga gatgccggtt aataaatgtt ttttaatttc ctgtgaaacc ggaattttat
     7861 tgtctttcat attaagatcc ctccagtata aggatcattt gtttcccagt gatttttcga
     7921 ccttttcaag aaattgaacg ggcgatttaa tgacgatctc tgtctttact cgcacaatac
     7981 gtttattggt gaagcgttct tctcctttta ttttcacatc tacagcaaga atcacgacgt
     8041 cagcagcaga gatatctgcc gcctgtaatt cattttctgt accaatggtt ccctgggttt
     8101 caactttaat ggtatggccc agagcttttg ctcctttgat taacttttca cgagcaatat
     8161 aggtatgagc aatacccgcc gtacaggcgg caacacagac aatattcatg acatactccc
     8221 tgttatttag cagacatcgg attgtgcata ctgactaaag agatcaatga ttttttgcgg
     8281 actcgtctca actaacaatt gttctatgat gtcatcatcc gccagcgcgc ccgcgacctg
     8341 ggaaagtaaa cggatatggg tggtattttg atcttcaaga cgtaccgcaa ataaaataat
     8401 acagcgcacg ctactgccat ccagcgtttc ccagggaata tcctggcggg tacggccaat
     8461 tgccagcgta gtattaatca cagctgaaga tttaccatgc gggatcgcaa tatgattttc
     8521 aaaaccagtc gagccttccg cttcgcgctg ccagacatct ttaataaagg tttcccgaca
     8581 atcgatcgca ccatcttttt tgagaagatt agttaattct tcaatcgcct ccgctttatt
     8641 cgtcgcttgc atatctaaaa taacgcgatt cgtattcagg atttttgtta tgtccattcg
     8701 tcatacctca taaaattctg acattaaatt tcaacgaccc gagacgtcgc atgatgtaat
     8761 ttttccgtaa tcaccatttc agtgacccgc ttaatatcgg catcattaaa aaaagcggta
     8821 acataagcga ccggcatagt gactgtaggc aaattaatgg tcgaaataat cagttcgata
     8881 ttgtcaggca aaacttgtga taaatttgct gctgaaataa catcaacaat cgtccattca
     8941 ggaaatgctc gcagaatacg gcttttcagt agatgcgaag ttccgattcc ggttgaacag
     9001 accagtaata cacgtttacg ggcaatttgc cgctccatcg cagcctgaaa atgaacggtc
     9061 aggtaaccca cttcatcctc gctcactgca ttctcacccc aggttttaaa tacctgattc
     9121 accacatatt gtgtcaaccg ccagacatcc gctaattctg ctttaatatc ttccagcaat
     9181 ggattacgga tatgaatgcg ataatttaga cggttaatca gcggcttaat atgcaccaat
     9241 aagccatcat ataatgcgct gtcttcacta aaatcgcagt ccacgatgtc actgaatacc
     9301 gtaattaaac gccaggtaat cagacgcgct tcattgctgg cctgcatatg tgaaataatg
     9361 ctaacatctt tttgtccatc aatcgccaca cctgatgaaa tgatatattg ataaataaac
     9421 cagacttcat catcgggcaa tgtatgtgca attcgttgtt ctatcttatg aatcatctga
     9481 ctggcaacat taaagatatt ttcatcatat tgcctggttt gatcttcttc tcttgataac
     9541 gcattcccgt gcgtgttgcg atacatcata ataaggatgt gagtaaaaat gttaacgtaa
     9601 taaggttctc ccaaagacca acttaattca ttttccatat ccagtaacaa tgactggaca
     9661 aataacacct cttcttctcc aaaataatgg actaatgctt tataacttcc gggatccaga
     9721 cgtgaataca cgacaccttg tggctcatta tggttaataa tgccgttaat cagtaatgcc
     9781 attgcctgtc ggacttgccc ttcactacct tcaatatgcg taccacttgg gctgcggatc
     9841 aacgataacc ccaacggcgc aagccaggac tcaattactc tcaggtcatt aacaatagag
     9901 gctccgctga taaagtagcg ttctgacaat ttactgatcg acgtttcatt cggtgtttca
     9961 cttaataact gcgacgctat tttaacgcgc ctggcattat tcattacacc atcaatctct
    10021 tcacattctg ctcccaacaa atgatcaaga tgcagcaaat cagcaatatt ctccgcgctt
    10081 aacatcacac ccgcgccagc acgcttctca acatttattc tccattgccc cagccattgc
    10141 tcaagtaaac gtaaatcacg ttgaatggtt ttttccgaaa catttaaata gccggcaatc
    10201 gtaacaatag tcgtatattc ccttcgaggt aaaaggaatc gtaataatct gttttgtcgc
    10261 gaggtaatca tttgcatttt ttacccttat cacagattaa gaagccccat gaggaagagc
    10321 ttcagttgcc atctctgagg gtaaatataa agcagaataa tttcacaaca atactaaaat
    10381 ctgtccgtaa tccggacagt gataaaacaa agcaaggcgg ttaagggata tttttcagca
    10441 cagatcacat tttcgcgatg aataatcgtg atcatgctcg caaatacgat ggaagaaata
    10501 tttattacca gatgtgagat tatttaccgg atacgacaca aaaccattcg tatccggcat
    10561 tcttcaatag attacaacgt aattgtcagc gcattccctt gaggagtcac caccagtccc
    10621 tgctcacttt cagcctgcga accgccttgc agaccattta ctttcacaat attgcgcaag
    10681 cacagtgtcc agttcttcgc ctcgcccgta ccaatcacag taatcgtgtt gccagtacgt
    10741 gcggctttta aagtaaagat caccgaaccg tcagcatcgg gcacttcaca gacggcttca
    10801 tgcccgtctt gcagattaaa gaggtggaat gctgtacctt cgtgccacgc gtaatcggga
    10861 cgttgatcgt tgttgcccag cgccagtagg gcgctatcac gcacataaac gggcagactc
    10921 aggaagctgt gctgctgttt atgccagcga ctaccgtcga gttcatcgtt gtgccacagg
    10981 tgtgtccagc gaccttccgg caggtagaac tgcacatcgc ccgcttcagt gaacacaggc
    11041 gcaaccatca cgttgtcgcc taacatgtat tgacggtcaa ggtaatcaca agccggatcg
    11101 tccgggaact ccatcatcat ggcccgcatc atcggcgtac cccgggcgtt tgcacgtgca
    11161 gcttcacgat acagatacgg catcatgcgg catttcagtt gcgtgaagaa gcgcaccaca
    11221 tcacaggact catcatcgta ggcccacggc acacgataag atttgctgcc atgtaaacgg
    11281 ctatggctgg aaagcaaacc aaacgcgcac cagcgtttgt aaacgtgcgc cggagcggta
    11341 ttttcaaagc cgccgatatc gtggctccag aagccaaaac ctgaaaggcc aatagacaaa
    11401 ccaccgcgca ggctttccgc cattgattcg tagttagcgt aacagtcgcc accccagtgt
    11461 accgggaatt tctgcgcacc gacggaggcc gagcgggcaa acaagacggc ttcttcctca
    11521 ccaacggtgt ccttgagcac gttccatacc agttcgttgt agatgtacgc ataatggtta
    11581 tgcattttct gcggatcgga accatcaaac cattgaacat cggttggaat acgttcgcca
    11641 aagtcggtct taaagcaatc aacgcccatc gcgaccagac ctttcagttt gtcggcgtac
    11701 catttgcagg catccggatt ggtaaagtca taaatcgcca gacctggctg ccatttatcc
    11761 cactgccaca gcgaaccgtc cgggcgtttg agtaaataac ctttctcttg taactcttta
    11821 aagacggggg atttttgccc aatatagggg ttaatccaga cgcagatttt cagccctttc
    11881 gctttcagac ggcggatcat cccttccggg tccgggaaag tcagcgggtc ccactcaaaa
    11941 tcgcaccact ggaaggcttt catccagaaa cagtcaaagt ggaaaacatg cagtggcaga
    12001 ttgcgttccg ccataccatc gataaagctg tttaccgtcg cttcgtcgta gttggtggta
    12061 aatgaagtgg ttagccacag gccgaaggac cacgcgggcg gcagcgccgg acgaccagta
    12121 aaacgggtat aacgatcaag taccgctttc ggcgtcgggc cgtcgataac aaagtattcg
    12181 agatattcac tctcaacgct gaactgcact ttggagactt tctccgatcc cacttcaaaa
    12241 gagacgcatt gaggatgatt gaccagtacc ccataaccac ggttagtcat atagaacgga
    12301 atatttttat acgcctgttc agtacttgtg ccgccgtccc ggttccaggt ctctaccgtc
    12361 tggccattgc gcaccagggc agtaaagcgc tctcccagac cgtaaactgt ttcgccaacg
    12421 ccaagatcca gccgctcaaa catataattt cgttgattat tcgtgtcctg cacgtagcca
    12481 ttatttttca cctgactacc ggtaatacgt tcgccgttgc gcagaaaatc cagtgaccag
    12541 aactcacctt tgctgacacg cgcgcttaag ttgccacttt taaactcagc gtaacgttct
    12601 gtgttttcga ttgtgacctt cacgtcctgc aaaatattga gcggataatg aggaccgtta
    12661 ttcagcgccc cctgaaaatg ctcaatccgt acaccgacaa taccttcctg tggggagaaa
    12721 aagcgcagcg taaataaagg agtatcaagc tgccaggtac gttcacgcac atcacgaggg
    12781 gcagcataga ccaccatttc attaccctgc tgttcaacct cgaacacctg aagcgggtga
    12841 atcaaattga ggccaggttg aatcaaccag tttccgtcgc taattttcat tgccgttcct
    12901 tagttctgca cttcttgaga ggtgaattgt tgctggcaac ggcgtttgcc ctgcgccagc
    12961 tgttccataa cggttttcag attgtgcgtc gtgagcgagt agtagcgttt agcgataatc
    13021 gcgctcagca agtaacagat cgccggaaca atcgtgaata gcgcgataat gatgctaatt
    13081 gtgacgctgt tctgcgcttt ttctgccgca tcatatccgc cataagccag catccagccg
    13141 ataagggcac caccgaaggc caaccccagt ttgagcacaa acagcgtgcc agcaaaactg
    13201 atcccggtca ggcgtttacc attgcaccac tcgccgtagt cgacggtatc ggacatcatt
    13261 acccactgga taggtgtcac cagttgatgc aacacaccaa tcacgaagat gaagacaaac
    13321 atggtgatgc tggcctgcat aggaacaaag aacatcgcga ggctaatcac tgccagcagg
    13381 gcgttcgtcc accagaagat agtgacttta catttccagt cggtcagcgg tttcgccagt
    13441 gcggaaccaa tcaggttacc cacgcaataa gtggtgagaa aagcgacaaa cacttccggc
    13501 gtacccaaaa tccatgtgac gtaatacatc atcgccccac cgcgtacgca caccgccagg
    13561 atattgaaaa ttgtcaggaa accgacaata cgccactggt cgttttgcca gatatcacgt
    13621 aaatcatccc gcatagacgt cgttgtaggt ggtgcttcaa cgcgttcttt agtggtgaag
    13681 aaacagaatg ccagcatcat aaatgccacc acggaaagga ccgcgatacc gccctggaaa
    13741 ccgagtggtt tattatcacc accaattaaa ttaaccagtg gcatcatcag aacagtagag
    13801 agcatgcctc ccgcggtcgc cagcacaaaa cgccaggatt gcagcgagat acgctgagtc
    13861 gggtcattgg tgattacacc acccaatgcg cagtaaggga tattgacgat ggtataaagt
    13921 aaggtaagta gggtataagt aattgctgca taaatcattt ttccgttcat actgagatct
    13981 ggcgtgctat aggccagtac acagacgatc ccgaatggca gtgcgccaaa cagcacccac
    14041 ggccgaaatt taccccagcg agagcgcgtt cgatcggcca acaaccccat gcaaggatcg
    14101 gaaatcgcat ccagtgcacg agcgaccaaa aacatggttc cgacaaatcc ggcaggaatg
    14161 ccaaaaatat cggtataaaa gaacatcata tataacatta cgttatcgaa aataatgtgg
    14221 ctggcggcgt ctcccatgcc ataaccaatt ttctctttaa cggacaacac ttcactcttc
    14281 atctgctttt ccttgatgct ataggggtgc tgagaccgat aacgcatttt gtaaaccatc
    14341 cctgcgagag cgaatattcc gatatctggt tattaattta tgattcttgt tttatgtgat
    14401 cgtggtagcg ttaatttcgc tcatatatca ttgtaaaata tgagttttat atgaactata
    14461 atgttttcgt gataatacgc tgcgtgtatt aggcggaaaa aactgatctg ggggatgtag
    14521 aaactcaagg aagtagctat aatgcgcccc gcctccatgt agcaatcgag gcgcggaaga
    14581 tcgtcgtctc cggtgaggcg gctggacttc aaatccagtt ggggccgcca gcggtcccgg
    14641 gcaggttcga ctcctgtgat cttccgccaa aatgcctctt ctgaactctc ccgacgttaa
    14701 caaaacccct tctaatccct tatatatcag gctttctttg tgtccagaga tcaatcgact
    14761 caacctgtat caagttgctt ttggggatac tttagggggt attatttcct cttgaaatat
    14821 tagcctccca taataggaaa aaggtaatac ataacaatgg tttttaacag catctttgtt
    14881 atacaagggg gcattgagga tatcaggaaa aatctcaaaa tcggtgcgga caggaaacta
    14941 cgtgggcagc aaaaacgtat ggtaacgccg ggaaatgcaa cgcccaccag tcgggctaat
    15001 ccaccactcc aatcgtggtt cgcagtactg cacacacgat taccgggcta tacaggagcg
    15061 gtttggtctg aaaacatcca tgccgcgtaa aggtaaccgt tacgacaaca cctcaatgaa
    15121 aaacttatag taaacgctga agaatgaaac tatcgtttta ataaccggga tgaagccatc
    15181 taagtaatac ggaaatacat tgagattttc tacaaccatc agcgccgcca ctcccgtctg
    15241 gggaatatct ccctggtagc cttcaggaaa aatataacca gataactgcg caaaaaaaga
    15301 acaaatggtg gtgtccgcta ttgccagtat acctgagttt actgcctttc agattatcac
    15361 aatcaatagc gaacagactt ttgagctctg tgcgctttta acaaaacgtc tctttctcca
    15421 ctttcaccgt ataataatac
//
LOCUS       DADSYM010000120        15275 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2415
            SAMN08039579-rid11723173.denovo.121, whole genome shotgun sequence.
ACCESSION   DADSYM010000120 DADSYM010000000
VERSION     DADSYM010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039579
            Sequence Read Archive: SRR6362533
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15275)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15275)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980162.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 03:39:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,484
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 4,347
            CDSs (with protein)               :: 4,347
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 43 of 85
            Pseudo Genes (incomplete)         :: 37 of 85
            Pseudo Genes (internal stop)      :: 19 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15275
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039579-rid11723173.denovo.121"
                     /strain="LGE2415"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8302 N 95.9628 W"
                     /collection_date="2015-08-24"
     gene            85..579
                     /locus_tag="J1Y83_003264"
     CDS             85..579
                     /locus_tag="J1Y83_003264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1441 family protein"
                     /protein_id="HBA7631383.1"
                     /translation="MDRELKNLTLNISQLAALSGVHRQTAAARLQNLPVAGGHESNLK
                     LYRVVDIVSAFLALPPPVAEGEMDAHERKAWYQSERERLKFEQETAQLIPASDVRREF
                     AIWAKAVVQVLETLPDILERDCGLQPAAVSRVQSIIDDLRDQIALRVTEAGADDEEEL
                     QQEE"
     gene            579..2681
                     /locus_tag="J1Y83_003265"
     CDS             579..2681
                     /locus_tag="J1Y83_003265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit family protein"
                     /protein_id="HBA7631384.1"
                     /translation="MLNQETAKAARTDSGYILRAPRRMRVADAVAQYMRVPMGAGNSV
                     PWDPLVAPYVIEPMNCLASREYDAVIFVGPARTGKTIGLIDGWVIYNVICDPADMLII
                     QMTEEKAREHSKKRLARTFRVSPEVVSRLSPNKNDNNVYDRTFLAGNYLKIGWPSVNI
                     MSSSDYKCVALTDYDRFPEDIDGEGDAFSLASKRTTTFMSSGMTLVESSPGRDVKDVK
                     WRRTSPHEAPPTTGILSLYNRGDRRRWYWPCPHCGEYFQPCGDVVAGFRDIADPVLAS
                     EAAYIQCPSCSGRILPEQKRELNGRGVWLRDGESINADGSRYGDPRRSRIASFWMEGP
                     AAAYQTLSQLVYKLLTAEQEYETTGSEETLKTVINTDWGLPYLPRASMEQRKSELLEQ
                     RAEPVPSRSVPDGVNFLVATVDVQAGRHRRFVVQVTGYGSRGERWIIDRYNITQSLRG
                     DSDGESQRIDPVSYPEDWDVLLTDVFHKSWPLASDPSQQMRLMAMAVDSGGEDGVTDN
                     AYKFWRRCRRDGLGKRIYLFKGDSIRRAKLITRTFPDNTGRTGRRAQAAGDVPLWLLQ
                     TDALKDRVNNALWRDSPGPGYVHFPDWLGSWFYDELTYEERSSDGKWSKPGRGANEAF
                     DLMVYAEALVILHGYEKIRWPDAPEWASRETWLECVPDSTEPSPSPEPVPTPVKKQKR
                     KKTVTDDVNPWLTSGGWL"
     gene            2678..2890
                     /locus_tag="J1Y83_003266"
     CDS             2678..2890
                     /locus_tag="J1Y83_003266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001072975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA7631385.1"
                     /translation="MNQNDIEAMIQRYTEAEMAVLDGKSVTFNGQQMTMENLSEIRQG
                     RQEWERRLAALITRRRGHPGYRLARF"
     gene            2890..4398
                     /locus_tag="J1Y83_003267"
     CDS             2890..4398
                     /locus_tag="J1Y83_003267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HBA7631386.1"
                     /translation="MAILDDVIGVFSPGWKAARLRSRAVIQAYEAVKTTRTHKARREN
                     RTADQLSQYGAVSLREQARYLDNNHDLVIGVFDKLEERVVGKNGIIVEPHPVLRNGAI
                     ARDLAAEIRTRWSEWSVSPEVTGQFTRPMLERLMLRTWLRDGEVFAQMVSGRINSLTP
                     SAGVHFWLEALEPDFIPMTSDESNRLNQGVFVDDWGRPEKYLVYKSRPVSGRQMETKE
                     VDAERMLHLKFVRRLHQMRGTSLLSGVLIRLSALKEYEDSELTAARIAAALGMYIRKG
                     DGQSYETDGNDSKENERELTIQPGIIYDDLKPGEEIGMVKSDRPNPNLETFRNGQLRA
                     VAAGSRLSFSSTARNYNGTYSAQRQELVESTDGYLILQDWFIGAVTRPMYRAWLKQAV
                     VSGVIRLPRDLDRSSLYTAVYSGPVMPWIDPVKEAEAWKIQIRGGAATESDWVRAGGR
                     NPDDVKRRRKAEIDENRKLDLVFDTDPASDKGGSSAATKRQEPQHTDDQSEE"
     gene            4343..6195
                     /locus_tag="J1Y83_003268"
                     /pseudo
     CDS             4343..6195
                     /locus_tag="J1Y83_003268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308856.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease ClpP"
     gene            6282..6605
                     /locus_tag="J1Y83_003269"
     CDS             6282..6605
                     /locus_tag="J1Y83_003269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308857.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2190 family protein"
                     /protein_id="HBA7631387.1"
                     /translation="MAKNFVEEGKTVAIVASAAISSGDLVQVGDVFAVALTDIPQGET
                     GDGITEGVFMLPKLKTDDMKTGKKVYLKSGKVQLTNSGSDPLVGVVWADAGTSAEEVP
                     VKLNV"
     gene            6598..6873
                     /locus_tag="J1Y83_003270"
     CDS             6598..6873
                     /locus_tag="J1Y83_003270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA breaking-rejoining protein"
                     /protein_id="HBA7631388.1"
                     /translation="MFDPFSRLAARMDAITVRKMGKTASINDVDMTVIPGETLAELNA
                     LSGPAVSLVVFSSGYRPRRGDRVVYDGQQWTVTRHERFNGKPMIFIE"
     gene            6885..7463
                     /locus_tag="J1Y83_003271"
     CDS             6885..7463
                     /locus_tag="J1Y83_003271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBA7631389.1"
                     /translation="MKGLENAIRNLNSLDTRMVPQASAWAINRVAQKAVSVATRQVAG
                     NTVAGDNQVKGIPLKLVRQRVRVFKASPSGKMTARIRVNRGNLPAIKLGTARVRLARR
                     GGKLQYRGSVLKVGKYLFRDAFIQQLANGRWHVMRRIDGKNRYPIDVVKIPLSGPLTQ
                     AFEDARDHIIAAEMPKQLGYALKQQLRLWLTR"
     gene            7460..7861
                     /locus_tag="J1Y83_003272"
     CDS             7460..7861
                     /locus_tag="J1Y83_003272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBA7631390.1"
                     /translation="MNRHTQIRQAVLARLREQCGDSATFFDGLPAFIDAQELPAVAVW
                     LSDAQYTGKMTDEDDWQAVLHIAVFIRAQAPDSELDMWMESTIFPALNDIPALSGLID
                     TLIPLGFNYQRDNEMATWAMAEITYQITYTN"
     gene            7872..8615
                     /locus_tag="J1Y83_003273"
     CDS             7872..8615
                     /locus_tag="J1Y83_003273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBA7631391.1"
                     /translation="MTTPNPLAKTKGAGTTFWMYTGNGDAFANPLSDTDWLRLAMVKD
                     LQPGEMTADAEDDTYLDDEDADWKTTTQGQKSVGDTSATLAWRPGDSGQKKLVQLFDS
                     GEVCAFRIKYPNGTVDVFRGWLSSLGKTIASKDVMTRTVKISGVGRPYLAEEGTETVS
                     VTGLTVSPASASVKVGATTTLTFTVKPDGASDKAISVHSSDPQTATVTLNGLVATVKG
                     VKQGSVSIVGMTADGNFVAVTAVTVSAAG"
     gene            8676..9062
                     /locus_tag="J1Y83_003274"
     CDS             8676..9062
                     /locus_tag="J1Y83_003274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein G"
                     /protein_id="HBA7631392.1"
                     /translation="MFLKQDTFNYEKQSVVLSELSGLQRIEYLTFVQQRTAKFDAGEG
                     ELPEAERQIAFLRMGMDINAWLVSRSLWNADQSKDVETLCASVITTWSYDALGAGAEM
                     VLSLSGMGAIDNAVDDEHEALTPEKS"
     gene            9071..9400
                     /locus_tag="J1Y83_003275"
     CDS             9071..9400
                     /locus_tag="J1Y83_003275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein T"
                     /protein_id="HBA7631393.1"
                     /translation="MQFVMRLAREFRRADWRRMLSEMSATELGEWGDYFRMQSFSDVW
                     MDAQFASLKALIVRMVSGSSDAAVADFSLLPEENGIPERTDEELMHLGEGISGGVRYG
                     PDSQPGH"
     gene            9372..12437
                     /locus_tag="J1Y83_003276"
     CDS             9372..12437
                     /locus_tag="J1Y83_003276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HBA7631394.1"
                     /translation="MDQIANLVIDLGIDAAEFKNEIPRIKNLLNGAASDAERSSARMQ
                     RFMERQTQAARQTTQAASSAATAASVHAQTVEKNAQAHERMAREVEKTRQRMEALSQK
                     MREEQAQAMALAEAQDKAAAAFYRQIDSVKQASAGLQELQRIQQQIRQARNSGGIGQQ
                     DYLALISEVTAKTRVLTQAEEEATRQKVAFIRQLKEQATRLNLSSSELLRAKAAQLGV
                     SSAAEVYIRKMEQAGKATHSLGLKSAAARQEIGVLIGELARGNLGALRGSGITLANRA
                     GWIDTLMSPKGMMPGGVIGGIAAAVYGLGKAWYDGQKEGEEFNRQLSLTGHYAGVTAG
                     QLWTLSRAISGNGITQHAAAGALAQVVGSGAFRGNDIGMVARAAAQMERSVGQSVSDT
                     INQFKRLKDDPVNAAKALDNELHFLTATQLEQIRVLGEQGRSSDAARIAMSALAEETG
                     RRTADIDNNLNALGSTLKYLSDLWSRFWDAAMNIGREDSLDEQIAALQEKVSRAKRLP
                     WTASSSQVEYDQQRLNDLQEKKRQKDLQDAKEQAERNYQEQQKRRNAENAALNRMNET
                     EAARHQREIARINAMQYADQAVRDAAIQRENERYEKALASGKKKTRETRNDEATRLLL
                     QYSQQQAQVEGQIAAARQSAGIATERMTEAHKQLLALQQRISDLDGKKLTADEKSVLA
                     RKDELIQALTLLDVKQQELQKQTALNELKKKTIQLTSQLAEEERAQRQQHDLDIATVG
                     MGDQQRQRYQVQLSLRQKYQQQLEQLRRDSEQKGTYNTDDYRKAEQALTESLNRQLNE
                     NRRYWQQLEVVQGNWKNGVLRAFQDFTVDADNTAETAEQVFSSAFSNMGNGLATFVTT
                     GKLNFKSFTSSVLSDMAKILAQATMMKSIKGIGSVLGFDLSSLSLNANGGIYQSADLS
                     RYSGTVVNRPTFFAFAKGAGVMGEAGPEAILPLRRGADGKLGVVADIGGSGMAMFSPQ
                     YNIEINNDGTNGQIGPAALKAVYDLGKKAAADFMQQQARDGGRLSGAYR"
     gene            12437..12766
                     /locus_tag="J1Y83_003277"
     CDS             12437..12766
                     /locus_tag="J1Y83_003277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBA7631395.1"
                     /translation="METFHWKVRPDMNVVSEPKVVTVKLGDGYEQRRAAGLNNQLSTY
                     SVTIRVRKCEHPSLKAFLERHGGVRAFQWTPPYDWKPIRVVCRKWSASVGALWVTITA
                     DFEQVVA"
     gene            12776..13474
                     /locus_tag="J1Y83_003278"
     CDS             12776..13474
                     /locus_tag="J1Y83_003278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HBA7631396.1"
                     /translation="MQDIPQETHHETTRLTQSAQVVLWEIDLTEVGGERYFFCNEQNE
                     KGEPVTWQGRQYQAYPIQGTGFELNGKGSAARPTLTVSNLHGMVTGMAEDLQSLVGGT
                     VVRRKVYARFLDAVNFVNGNSDADPEQEVISRWRIEQCSELSAVSASFVLSTPTETDG
                     AVFPGRIMLANTCTWTYRGGECGYHGPAVADEYDQPTSDITKDKCSKCLSGCKFRNNV
                     GNFGGFLSINKLSQ"
     gene            13480..14223
                     /locus_tag="J1Y83_003279"
     CDS             13480..14223
                     /locus_tag="J1Y83_003279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBA7631397.1"
                     /translation="MTETESAILAHARRCAPAESCGFVVRTPEGERYFPCVNISGEPE
                     AYFRMSPDDWLQATLHGEIVALVHSHPGGLPWLSEADRRLQVQSDLPWWLVCRGAIHK
                     FRCVPHLTGRRFEHGVTDCYTLFRDAYHLAGTELPDFHREDDWWRHGQNLYLDNLEAT
                     GLYQVPLSAAQPGDVLLCCFGSSVPNHAAIYCGDGELLHHIPEQLSKRERYTDKWQRR
                     THSLWRHRAWHASAFTGICNDLAAASTFV"
     gene            14160..14768
                     /locus_tag="J1Y83_003280"
     CDS             14160..14768
                     /locus_tag="J1Y83_003280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019843089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HBA7631398.1"
                     /translation="MARICLYGDLQRFGRRIDLRVKTGAEAIRALATQLPAFRQKLSD
                     GWYQVRIAGRDAGETELSARLNEPLANGAVIHIVPRLAGAKSGGVFQVVLGAALIAVA
                     WWNPVGWLGAAAVSGMYAAGASMILGGVAQMLAPKARTPTAASTDNGKQNTYFSSLDN
                     MVAQGNVLPVLYGEMRVGSRVVSQEISTADEGDGGQVVVIGR"
     gene            14829..>15275
                     /locus_tag="J1Y83_003281"
     CDS             14829..>15275
                     /locus_tag="J1Y83_003281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host specificity protein J"
                     /protein_id="HBA7631399.1"
                     /translation="MGKGSSKGHTPREAKDNLKSTQLLSVIDAISEGPVEGPVEGLKS
                     VLLNSTPVLDNEGNTNISGVTVVFRAGEQEQTPPEGFESSGSETVLGTEVKYDTPITR
                     TITSANIDRLRFTFGVQALVETTSKGDRNPSEVRLLVQIQRNGGWVT"
BASE COUNT         3628 a         3569 c         4720 g         3358 t
ORIGIN      
        1 attttgattg ttttttgttc atcactaaca ccgtttgcct aaagtcgttc gcaagatgca
       61 tgtttaaaac attctggagc gggtatggat cgagagttaa aaaatctgac gctgaatatc
      121 agtcaattgg cggcactgtc aggtgtacat cgccagactg ctgcggcaag gctgcaaaat
      181 ctacccgttg caggggggca tgaaagcaac ctcaagcttt atcgggtggt tgatattgtg
      241 tcggcatttc tggcattacc accgccggtt gcagaaggcg aaatggacgc acatgagcgc
      301 aaagcctggt atcagtctga acgtgagcgt cttaagttcg aacaggaaac ggcacaactc
      361 attccggcca gtgatgtcag acgggagttt gccatctggg caaaagcggt cgtgcaggtg
      421 ctggagacat taccggatat tcttgaacgt gactgcggtc tgcagcctgc cgctgtgagc
      481 cgtgttcagt ccattattga tgatctgcgc gatcagatag ccctgcgggt gactgaagca
      541 ggtgcggatg atgaggagga attacagcag gaggagtaat gctgaatcag gaaaccgcaa
      601 aggcagcacg aaccgattca ggttatatcc ttcgcgcacc gagacgaatg cgggttgctg
      661 atgccgttgc tcagtatatg cgggtgccca tgggggcagg gaactcagtc ccgtgggatc
      721 cgctggtggc accgtatgtt attgagccta tgaactgcct ggcctcgcgt gaatacgacg
      781 cagtgatatt tgttggcccg gcacgaaccg gcaagactat cggcctgatt gacggctggg
      841 tgatttacaa cgtgatttgc gatcctgctg atatgctgat cattcagatg acggaggaaa
      901 aagcccgcga acactccaaa aaacgactcg ccagaacgtt tcgcgtcagc ccggaagtgg
      961 tcagtcgcct gagtccgaac aaaaatgaca acaacgttta tgacagaaca ttccttgctg
     1021 gcaactacct gaaaatcggc tggccgtcag tcaatatcat gtcttcatca gattataaat
     1081 gcgtggcgct gacggattat gaccgttttc cggaagatat tgatggtgag ggggatgcct
     1141 tctctcttgc ctcaaaacgt accaccacat ttatgtccag tggtatgacg cttgtggaga
     1201 gttcccccgg cagggatgtg aaggatgtga aatggcgacg gacttcaccg catgaggctc
     1261 caccaaccac ggggatcctg tcgctctata accgtggcga tcgccgtcgc tggtactggc
     1321 cctgtccaca ctgtggtgag tattttcagc cctgcggcga tgtggttgct ggtttccgtg
     1381 atattgccga tcctgtgctg gcaagtgagg cggcttatat tcagtgtcct tcctgttcag
     1441 gacggatttt gcctgaacaa aaacgcgagc tgaacggacg tggggtctgg ttacgggatg
     1501 gtgaatccat caatgcagat ggcagtcgtt atggtgatcc ccggcgttca cgtattgcgt
     1561 cattctggat ggagggtccg gcagctgctt atcagacact ctcgcaactc gtttacaaac
     1621 tgcttactgc agaacaggaa tacgagacaa ccggaagtga agagacactc aagacggtta
     1681 tcaataccga ctggggatta ccttatcttc cccgcgccag catggagcaa cgaaaaagtg
     1741 aattgcttga gcagcgggca gagccagttc cttcccgcag tgtgccggat ggcgttaatt
     1801 tccttgtggc gacagtggat gtgcaggcgg gacgtcatcg ccgttttgtg gttcaggtaa
     1861 cgggctatgg cagccgtggc gaacgctgga ttattgatcg ttacaacatc acgcagtcat
     1921 tgcgcggtga cagcgacggg gagagccagc gaattgatcc ggtcagctat ccggaagact
     1981 gggatgtcct gctgacggat gtttttcata aaagctggcc gctggcctcc gacccttctc
     2041 aacaaatgcg actgatggca atggcggtgg actccggcgg tgaagacggg gtcactgata
     2101 atgcttataa attctggcgt cgttgccgtc gtgatggcct tggtaaacgt atttacctgt
     2161 ttaagggcga cagcatccgg cgagcaaaac tgatcacccg tacattccct gataacaccg
     2221 gacgaacggg ccgacgggcg caggccgcag gtgatgtgcc gctctggctt cttcagacgg
     2281 atgccctgaa agaccgggtg aataacgcgt tatggcgtga ctcgccaggt cccggctatg
     2341 tgcatttccc tgactggctg gggagctggt tttacgacga actgacgtat gaagagcgga
     2401 gcagtgacgg gaaatggagt aagccgggtc gcggtgccaa cgaagctttt gacctgatgg
     2461 tgtatgccga ggctctggtc attctgcatg gatacgaaaa gatccgctgg ccggatgcac
     2521 cggagtgggc gagccgggaa acctggctgg agtgtgtccc ggacagtacc gaaccgtcac
     2581 cctcaccgga accggtaccc acgcctgtta aaaaacaaaa acggaagaaa acagtaactg
     2641 acgatgttaa cccctggctg acttccggag gatggttatg aatcagaatg atattgaagc
     2701 catgattcag cgttatacgg aagctgaaat ggcggtgctg gacggaaaat ccgtcacttt
     2761 taatggtcag cagatgacca tggaaaactt atctgagatc cggcaggggc ggcaggagtg
     2821 ggagcgccgc cttgcggctc tgattacacg acgacggggg catcccgggt accggctggc
     2881 gaggttctga tggcaattct tgatgatgtg attggcgttt tttcaccagg atggaaagcg
     2941 gcaaggctgc gttcccgtgc ggtgatccag gcttatgagg ccgtaaaaac gacgcggaca
     3001 cacaaagccc gacgggagaa ccgaactgcc gaccagttaa gccagtacgg ggccgtgtcg
     3061 ttacgtgagc aggctcgtta ccttgataac aaccacgatc tggtcattgg tgtatttgac
     3121 aagctggaag aacgggtggt ggggaaaaac gggattattg tcgagccaca tccggtatta
     3181 cgcaatgggg ccattgcccg tgatctggca gcggagatac gtacccgatg gagtgaatgg
     3241 tctgtcagtc cggaggtcac cgggcagttt acccgtccga tgctggaacg tctgatgctg
     3301 cgtacctggc tgcgcgatgg tgaggtgttt gcccagatgg tttccgggcg cataaacagc
     3361 ctgacgcctt ctgccggtgt tcatttctgg ctggaggcgc tcgagccgga ctttattccc
     3421 atgaccagtg atgagagcaa caggctgaat cagggcgtgt ttgttgatga ctgggggcgt
     3481 cccgaaaaat atctggtgta taaaagccgt cccgtatccg ggcggcagat ggaaaccaaa
     3541 gaagtggatg cagagcgaat gctgcatctt aaatttgttc gccgtctgca ccagatgcgc
     3601 gggacgtctt tgttgtccgg tgtgctgatc cgcctcagtg ctctgaaaga gtatgaagat
     3661 tctgagctga ctgcagcaag gatcgccgct gctctgggga tgtacatccg gaaaggcgac
     3721 ggacagagct atgaaacgga tggtaatgac agcaaggaga atgaacgcga gcttaccatt
     3781 cagccaggca ttatttacga cgatctgaaa cccggcgaag aaatcggaat ggtgaagtcg
     3841 gatcgcccca atcctaacct tgaaactttt cgtaatggtc agttgcgtgc cgtggctgcg
     3901 ggcagtcgtc tgagtttttc cagtacagcg cgcaactata acggcactta cagcgcccag
     3961 cgtcaggagc tggttgaatc tactgatggc tacctgatcc tgcaggactg gtttattggt
     4021 gccgtcaccc gcccgatgta ccgtgcctgg ctgaaacagg ctgtggtatc cggtgttatc
     4081 aggctacccc gcgatctgga ccgatcttca ctgtataccg cggtgtattc cggaccggtg
     4141 atgccgtgga ttgaccctgt taaggaggct gaggcctgga aaattcagat tcgtggtgga
     4201 gcggcgacag aatcagactg ggtacgtgca ggtggtcgta atccagatga tgtcaaacgt
     4261 cggcgcaagg ccgaaattga tgaaaaccgc aagctggatc tggtatttga taccgatccg
     4321 gccagtgata aaggaggcag cagtgccgca acgaaacgac aggagccgca gcacaccgac
     4381 gaccagtccg aagaataatt cctggttcag gatgcaggca ggtcaccaga gtgacgcgga
     4441 tatttatatt tatgacgaga ttggtttctg gggtgttacg gcgaagcagt ttatcagtga
     4501 tctgaatgca ctgggcgata tcacccacat taatctccat attaattcac cgggtggcga
     4561 tgtctttgaa ggcatcgcca tttttaatgc actgaaaaca catggtgcgt ccattaccgt
     4621 ttatgtcgac ggtgtggcgg cgtcaatggc gtcggtcatt gcgatggtgg gaaacccggt
     4681 cattatgccg gaaaactcct tcatgatgat tcataaacca tttggcttta cgggcggtga
     4741 tgcggaggac atgcgcacct atgccgacct gctcgataaa gttgaggcgg ttctgttacc
     4801 cgcttatgca cagaaaaccg ggaaaaccac cgatgaaatt gctgccatgc tggcggatga
     4861 gacctggatg tccggtgccg aatgtctggc acagggattt gctgatcagg tgacaccagc
     4921 cgttaaggca atggcatgta ttcagtcaaa acgtacagag gaatttaaaa aaatgatctg
     4981 tttgccatgt ttggcgggcg ttatcagtcg ttgcaggctc agtgtcttgc cgatcctgaa
     5041 tgttcgctgg agcaggcccg cgaaaagctg ttgaacgaga tggggcgcga gtccacgcca
     5101 tccaataaaa ataccccggc tcatatttat gccggtaacg gtaattttgt gggggacggg
     5161 atccgccagg cgctgatggc gcgcgccggg tttgaaaaaa ctgaacgtga taatgtctac
     5221 aacgggatga ccctgcgtga atatgcccgt atgtcactga ctgaacgtgg tattggtgtt
     5281 tccagttata acccgatgca gatggtcggt gcggcgttca cacacagtac gtctgacttc
     5341 ggtaatattc tgctggatgt tgcgaacaaa gccattctgc agggctggga agatgcccct
     5401 gaaacctatg aacagtggac gcggaaaggt cagttgtctg attttaaaat tgcccatcgt
     5461 gtgggtatgg ggggcttcag tgctctgcgt caggtgcgtg aaggggcaga atataaatac
     5521 gtcaccaccg gagataaaca ggccactatt gcactggcga cctatggcga gctgttcagt
     5581 atcacccgtc aggccattat caatgatgat ctgaatatgc tgaccgatgt cccgatgaag
     5641 ctgggccgtg cggcgaaatc cactattgcc gatctggttt atgccattct gacgtctaac
     5701 ccgaaaatct ccacagataa tgtaagtctg ttcgataaag cgaaacatgc aaacgtactg
     5761 gagagcgcgg caatggacgt ggcatcgctg gataaagccc gccagttgat gcgcgttcag
     5821 aaagagggtg agcgtcatct gaatattcgt cctgcgttcg tactggtacc gacggcgatg
     5881 gagtctgtcg ctaaccaggt cattcgctcc tcaagtgtca agggggctga cattaacgcc
     5941 ggtattatta acccggtgaa agattttgcg accgttattg cagagcctcg tcttgatgat
     6001 aacagccaga ccaccttcta cctggctgcg tccaaaggct ccgatacgat tgaagtggct
     6061 tatctcaacg gtgtggatac gccatatatt gatcagatgg agggcttcag tgtggatggc
     6121 gtgacaacga aagtgcgtat tgacgctggt gttgcgccag ttgatcaccg cggcctggtg
     6181 aaatgtacgg cgtaaacgtc gcagacaaca actctgatgg cccgtaaggg ctttttttgt
     6241 acctgaaatc agcccctgaa cggggctgtg cggagacagt tatggcaaag aattttgtag
     6301 aagaaggaaa aacggtggcg attgttgcca gtgcagccat cagcagcgga gatctggtgc
     6361 aggtgggtga tgtttttgcg gtggcgctga ccgatattcc acagggagaa acaggcgacg
     6421 gcataaccga aggtgtgttt atgctgccta agctgaaaac ggatgacatg aaaacgggta
     6481 agaaggttta tctgaagtca ggaaaagttc agctgactaa cagcggctct gatccgctgg
     6541 tcggggttgt atgggcagat gccggaacca gtgcagaaga agtgccggta aaactcaatg
     6601 tttgatccct tttcccggct ggcagcgcgt atggatgcga tcacggtcag aaagatggga
     6661 aagacagcct cgattaatga tgtcgatatg actgtgatcc cgggagaaac actggcagag
     6721 ctgaatgctc tgtccggacc tgcggtctct ctggtggtgt tttcttcggg ataccgccca
     6781 cggcgcgggg atcgcgttgt ttatgacgga caacaatgga cggtcacacg gcatgaacgt
     6841 tttaacggta agccaatgat ctttattgag taaagaggtg tgggatgaag gggcttgaga
     6901 atgccatccg caatctgaac agccttgata cccgtatggt gccacaggcc agcgcatggg
     6961 cgataaaccg tgtggcacag aaagcggtct cggttgccac ccggcaggtt gccgggaata
     7021 ccgttgcggg agataaccag gtgaaaggga tccccctgaa actggtacgt cagcgtgtcc
     7081 gggtgtttaa agccagtccg tcaggaaaaa tgacggccag gatccgcgtt aaccggggca
     7141 atctgcccgc cattaagctg gggacagccc gggtcagact ggcccggcgt ggtggaaaac
     7201 tgcagtaccg tggcagtgtg ctgaaggtgg gtaaatatct tttccgggat gcgtttattc
     7261 agcaactggc gaatggtcgc tggcatgtga tgcgacgtat tgatggcaaa aatcgttacc
     7321 ccattgatgt ggtgaaaatc ccgctgtccg gaccgctgac acaggcattt gaagatgccc
     7381 gcgaccacat cattgctgcg gaaatgccga aacagctggg gtatgcactg aaacaacaac
     7441 tgaggttatg gctgacccga tgaaccgaca tacacaaatc cgccaggccg tactggcacg
     7501 ccttcgggaa cagtgtggag acagcgccac gttttttgac gggcttccgg catttattga
     7561 tgcgcaggaa ctgcctgccg tggcggtgtg gctgagtgat gctcagtaca ccggaaaaat
     7621 gacggatgaa gatgactggc aggctgttct gcatattgcc gtcttcatcc gggcacaggc
     7681 accggattca gagctggata tgtggatgga gagcaccatt ttccctgccc tgaatgatat
     7741 accggcactt tccggactca tcgacaccct gatcccactc ggttttaact atcaacgtga
     7801 taatgagatg gccacctggg cgatggcgga aatcacgtac cagatcacgt acacgaatta
     7861 aggaggtggt aatgaccaca ccaaatccac tggcaaagac gaaaggtgcg gggacgacgt
     7921 tctggatgta taccggcaac ggcgatgcgt ttgcgaaccc tttgtcggac actgactggc
     7981 tgcgtcttgc gatggtgaag gatctgcaac ctggcgaaat gaccgctgat gcagaagatg
     8041 acacttatct cgatgatgaa gatgcagact ggaaaacgac aacccagggg cagaaatccg
     8101 ttggtgatac ttcggcgacg ctggcctggc gtccgggtga cagcgggcag aaaaaactgg
     8161 ttcagttgtt cgactccggt gaagtctgcg cgtttcgtat caaatatccc aacggcactg
     8221 ttgatgtttt ccgtggctgg ctgagctcac tgggtaaaac cattgcctca aaagacgtga
     8281 tgacccgcac tgtgaaaatc agcggtgtgg ggcgtccgta tctggcagag gaaggcactg
     8341 aaaccgtgag cgttaccggg ctgacagtat caccggcgtc tgccagtgta aaagtgggag
     8401 caaccaccac gctgaccttt acagtaaaac ctgacggagc cagtgacaaa gcgatcagtg
     8461 tgcattcgtc agatccacag actgccacgg ttaccctgaa cggacttgtg gccacggtaa
     8521 aaggcgtgaa gcagggcagt gtcagcattg tgggcatgac cgctgacggg aattttgtgg
     8581 ctgtgactgc ggtgactgtc agcgcagcag gttaacagga cgatactcat catttgcccc
     8641 ggttatgcgg ggcttttttg caggtggaga acatgatgtt tctgaaacag gacacgttta
     8701 attatgaaaa acagtccgtg gtgctcagtg agctgtccgg gctgcagaga attgaatatc
     8761 tgacgtttgt tcagcagcga acggcaaagt ttgatgccgg ggagggagaa ctgccggagg
     8821 ctgaacgaca gattgctttt ctgcggatgg ggatggatat caatgcctgg ctggtttccc
     8881 gctcactgtg gaatgcggat cagtctaagg atgtagagac gctttgcgca tccgttatta
     8941 caacatggtc gtatgatgcc ctgggtgcgg gggcggagat ggttctgtcg ctgagcggta
     9001 tgggggccat tgataatgcc gtggatgatg agcatgaggc gctgacgccg gaaaagtcct
     9061 gacgcgggaa atgcagtttg tcatgcggct tgcccgggag ttccggcggg cagactggcg
     9121 gcggatgctg tcggaaatgt cggccactga gcttggtgag tggggcgatt atttccggat
     9181 gcagagcttc agtgatgtgt ggatggatgc gcagtttgcc tcgctgaagg cattgatcgt
     9241 gagaatggtg tccggcagca gtgatgctgc ggtggctgat ttcagccttt taccggaaga
     9301 gaacgggata ccggagcgaa cggacgaaga actgatgcat cttggggaag gtatttccgg
     9361 aggtgtgcgt tatggaccag atagccaacc tggtcattga tttggggatt gatgcggcag
     9421 agtttaaaaa tgaaattccc cgtatcaaaa accttctgaa tggtgcagcc agcgatgcag
     9481 aacggtcttc tgcccgtatg cagcgtttta tggagcgtca gactcaggcc gcccggcaga
     9541 caacgcaggc agcttcttcg gctgcaacag ccgcatccgt ccatgcgcag acggtggaga
     9601 agaacgcaca ggctcatgaa cgcatggccc gcgaggtgga gaaaacccgc cagcgcatgg
     9661 aggcactgag ccagaaaatg cgcgaggaac aggcgcaggc catggctctg gcggaggctc
     9721 aggataaagc ggctgctgcg ttttatcgtc agattgacag tgtgaaacag gccagtgcgg
     9781 ggctgcagga attacagcgt attcagcagc agatccgaca ggccagaaac agtggcggga
     9841 ttggtcagca ggattatctg gcgctgattt ctgaggttac tgcgaaaacc cgtgttctta
     9901 cacaggctga ggaagaggct acccgacaga aagtggcgtt tatccgtcag cttaaagagc
     9961 aggcaacccg cctgaatctt tcatcttctg agttgcttcg tgctaaggca gcccagctgg
    10021 gggtaagcag tgctgcagaa gtgtatatcc gcaaaatgga gcaggcagga aaagccacgc
    10081 attcgctggg tctgaaaagt gcagcagccc gccaggagat aggcgttctg ataggtgaac
    10141 tggcccgcgg caatttaggg gcgctgaggg gatccgggat aacgctggct aaccgtgccg
    10201 gatggataga cacactgatg tcaccgaaag gcatgatgcc aggcggggtt attggcggta
    10261 ttgccgcggc cgtctatggt ctgggtaaag cctggtatga cggtcagaag gagggggaag
    10321 aatttaaccg ccagttgtcg ctgacggggc attatgccgg agtcactgcc gggcagctgt
    10381 ggacgctcag tcgtgctatt tccgggaatg gtatcacgca acatgctgca gccggtgcgc
    10441 tggctcaggt ggtggggagt ggtgcatttc gtggaaacga tatcggtatg gtggcgagag
    10501 ctgccgcaca gatggagcga tcggttggcc agtcggtcag cgataccata aatcagttta
    10561 agcggctgaa ggatgatcct gtaaatgccg cgaaggctct ggacaatgag ctgcattttc
    10621 ttactgccac tcagcttgag cagatacgcg tccttgggga gcaggggcgg tccagtgatg
    10681 ctgcacggat agccatgtct gcactggcag aggaaaccgg tcggcgtact gcggatattg
    10741 ataataacct caatgcgctt ggcagtacgc tgaagtatct gtctgatttg tggagtcgtt
    10801 tctgggatgc ggccatgaat attggtcgtg aagactcgct ggatgaacag attgccgctt
    10861 tacaggagaa agtgtcgcgg gcgaaaagac tcccctggac ggcatcatct tctcaggttg
    10921 aatacgatca gcagcgtctt aacgatcttc aggagaaaaa acgccagaag gatttgcagg
    10981 atgcaaaaga gcaggcagag cggaattatc aggagcaaca gaaacgccgt aatgctgaaa
    11041 atgctgcact gaaccggatg aatgaaacgg aagcagcacg acatcagcgt gaaattgcgc
    11101 gtattaatgc catgcagtac gccgatcagg ctgtcaggga tgcggcgata caacgtgaaa
    11161 atgaacgtta cgagaaagcc ctggcatccg gtaagaaaaa aacacgcgaa acccgtaatg
    11221 atgaggccac ccggttattg ctgcagtaca gtcagcaaca ggcacaggtg gaaggacaga
    11281 ttgctgctgc cagacagtca gcaggcattg ccacggaaag gatgacagaa gcgcataaac
    11341 agcttctggc tctgcagcag cgcatcagcg acctggacgg gaaaaaactg acggcagatg
    11401 aaaagagtgt gctggcccgt aaagatgaac tgattcaggc actgacgctg ctggatgtaa
    11461 aacagcagga gcttcagaaa cagacggcac tcaacgagct gaagaaaaaa acaattcagc
    11521 tgaccagtca actggctgaa gaagagcgcg ctcagcgtca gcaacatgac ctggatatcg
    11581 ccacggtggg tatgggtgat cagcagcgac agcgatatca ggtacaactg agtcttcgcc
    11641 agaaatacca gcaacagctg gagcagttga ggcgggatag tgagcagaaa gggacatata
    11701 acacggatga ctacagaaag gccgagcagg cgctgacgga gagcctgaac cgacaactga
    11761 atgagaatcg ccgttactgg caacagcttg aagttgtgca gggtaactgg aaaaacggag
    11821 tcctgcgtgc atttcaggat tttaccgtgg atgcagataa tacggcagaa acagcagaac
    11881 aggtgttctc gtcagccttc agcaacatgg gaaatggcct ggcaactttt gtcactaccg
    11941 gcaaactcaa tttcaaatcc ttcacctctt ctgtgctgtc agatatggcg aaaatcctgg
    12001 cgcaggcaac catgatgaaa tcgataaaag ggattggcag tgtactggga tttgatctca
    12061 gcagcctttc cctgaatgcc aatgggggga tttatcagtc tgctgatttg agtcgttaca
    12121 gtggcacggt ggttaaccgt ccgacgtttt ttgcttttgc aaaaggcgcg ggtgtgatgg
    12181 gggaagcggg acctgaagcc attctgccat tgcgtcgtgg tgctgacggt aagctggggg
    12241 ttgtggcgga tattgggggt tcaggtatgg cgatgttttc cccgcagtac aacatcgaga
    12301 tcaataacga tggcacgaac gggcagatag gtccggctgc cctgaaggcg gtttatgacc
    12361 tcgggaaaaa agcggcagcg gactttatgc aacagcaggc ccgtgatggt ggtcggttaa
    12421 gtggagcata tcggtaatgg agacgtttca ctggaaagtg cgcccggata tgaatgtggt
    12481 atcagagccg aaagtggtga cagtgaagct gggcgatggt tatgaacagc gtcgtgcggc
    12541 gggactgaat aaccagttgt cgacttacag cgtgacgata cgtgttcgta aatgtgaaca
    12601 cccatcttta aaagcctttc tggaacggca cggtggcgtc cgcgcatttc agtggacgcc
    12661 accttatgac tggaagccga tcagggtggt ttgtcgtaaa tggtcggcaa gcgtgggggc
    12721 gctgtgggta accataacgg cagattttga acaggtcgtg gcataggagg ctctgatgca
    12781 ggatattcca caggaaacac atcatgagac gacacgcctc actcagtcag cccaggtggt
    12841 gctctgggaa atcgatctga cagaggtcgg tggtgaacgt tattttttct gtaatgagca
    12901 gaacgaaaaa ggtgagccgg ttacctggca ggggcggcag tatcaggcat accccattca
    12961 ggggacggga tttgaactga acggcaaggg cagtgctgcc cgtccgacac tgacggtttc
    13021 taacctgcac ggcatggtca cggggatggc ggaagacctg cagagtctgg tcggcggaac
    13081 ggtggtcagg cgtaaggttt acgcccgttt tctggatgcg gtgaacttcg tcaacggaaa
    13141 cagcgacgcc gatccggagc aggaggtgat cagccgctgg cgcatcgagc agtgcagcga
    13201 actgagtgcg gtcagtgcct cctttgtgtt gtccacaccg acggaaacgg acggcgctgt
    13261 ttttccgggg cgcatcatgc tggccaacac ctgcacctgg acctatcgcg gcggtgagtg
    13321 cggttatcac ggtccggcgg tcgcggatga atatgaccag ccgacgtccg atatcacgaa
    13381 agataaatgc agcaaatgcc tgagtggctg taagtttcgc aataacgtcg gcaactttgg
    13441 cggcttcctt tccattaaca aactttcgca gtaaatccca tgacagagac agaatcagcg
    13501 attctggcgc acgcccggcg atgtgcgcca gcggagtcgt gtggcttcgt ggtgagaacg
    13561 ccggaagggg aaagatattt tccctgcgtg aatatctccg gtgagccgga ggcgtatttc
    13621 cggatgtcgc cggatgactg gctgcaggca acactgcacg gtgagattgt ggcgctggtc
    13681 cacagccacc ccggtggtct gccctggctg agtgaggccg accggcggct gcaggtgcag
    13741 agtgatttgc cgtggtggct ggtctgccgg ggggcgattc acaagttccg ctgtgtgccg
    13801 catcttaccg ggcggcgctt tgagcacggg gtgacggact gttacacgct gttccgggat
    13861 gcttaccatc tggcggggac tgaactgccg gattttcacc gcgaggatga ctggtggcgt
    13921 cacggtcaga atctctatct ggataatctg gaggccacag ggctgtatca ggtgccgttg
    13981 tcagcggcgc agccgggcga tgtgctgctg tgctgttttg gttcatcggt gccgaatcat
    14041 gccgccattt actgtggtga cggcgagctg ctgcaccata ttcctgaaca actgagcaaa
    14101 cgagagaggt ataccgacaa atggcagcga cgcacacact ccctctggcg tcaccgggca
    14161 tggcacgcat ctgcctttac ggggatttgc aacgatttgg ccgccgcatc gaccttcgtg
    14221 tgaaaacggg ggccgaagcc atccgggcgc tggccacaca gctccctgcg tttcgtcaga
    14281 aactgagcga cggctggtat caggtgcgta ttgccgggcg tgatgcaggt gaaaccgaat
    14341 tgtctgcccg tcttaatgag ccgctggcaa atggtgccgt gatccacatc gtgccgcgtc
    14401 tggcgggagc taaaagtggc ggtgtgtttc aggtggtgct gggggcggcg ctgattgcgg
    14461 tggcatggtg gaaccctgtg ggctggctgg gtgccgcggc tgtatcgggc atgtatgcgg
    14521 caggggccag tatgatcctg ggcggagtgg cgcagatgct ggcaccgaaa gccaggacgc
    14581 ccacggcagc aagtacagat aacggcaaac agaacaccta tttctcctca ctggataaca
    14641 tggttgccca gggcaatgtt ctgcccgttc tgtacggtga aatgcgcgtg gggtcgcggg
    14701 tggtctctca ggagatcagc acggcagacg aaggggatgg tggtcaggtt gtggtgattg
    14761 gtcgctgatg aaaaacgttt tatgtgaaac cgcctccggg cggttttgtc gtttatggag
    14821 cgtgaggaat gggtaaaggc agcagtaagg ggcatactcc gcgcgaagcg aaggacaacc
    14881 tgaagtccac gcagttgctg agtgtgatcg atgccatcag cgaagggccg gttgaaggtc
    14941 cggtggaggg attaaaaagc gtgctgctga acagtacgcc ggtgctggac aatgagggga
    15001 ataccaatat ctccggtgtc acggtggtgt tccgggcagg tgagcaggag cagactccgc
    15061 cggaggggtt tgaatcctcc ggttccgaga cggtgctggg tacggaagtg aaatatgaca
    15121 cgccgatcac ccgcaccatc acgtcggcaa acatcgaccg tctgcgcttt accttcggtg
    15181 tgcaggcact ggtggaaacc acctcaaagg gtgaccggaa tccgtcggaa gttcgcctgc
    15241 tggttcagat acagcgtaat ggtggctggg tgacg
//
LOCUS       DADSYM010000121        15027 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2415
            SAMN08039579-rid11723173.denovo.122, whole genome shotgun sequence.
ACCESSION   DADSYM010000121 DADSYM010000000
VERSION     DADSYM010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039579
            Sequence Read Archive: SRR6362533
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15027)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15027)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980162.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 03:39:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,484
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 4,347
            CDSs (with protein)               :: 4,347
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 43 of 85
            Pseudo Genes (incomplete)         :: 37 of 85
            Pseudo Genes (internal stop)      :: 19 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15027
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039579-rid11723173.denovo.122"
                     /strain="LGE2415"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8302 N 95.9628 W"
                     /collection_date="2015-08-24"
     gene            47..409
                     /locus_tag="J1Y83_003282"
     CDS             47..409
                     /locus_tag="J1Y83_003282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311492.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2799 domain-containing protein"
                     /protein_id="HBA7631400.1"
                     /translation="MKKFIAPLLALLVSGCQIDPYTHAPTLTSTDWYDVGMEDAISGS
                     AIKDDDAFSDSQADRGLYLKGYAEGQKKTCQTDFTYARGLSGKSFPASCNNVENASQL
                     HEVWRKGADENASTIRLN"
     gene            557..1075
                     /locus_tag="J1Y83_003283"
     CDS             557..1075
                     /locus_tag="J1Y83_003283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiR family protein"
                     /protein_id="HBA7631401.1"
                     /translation="MRFSHRLFLLLILLLTGAPILAQEPSDVAKNVRMMVSGIVSYTR
                     WPGLSGPPKLCIFSSSRFSTALQENAATSLPYLPVIIHTQQEAIISGCNGFYFGNESP
                     TFQMELTEQYPSKALLLIAEQNTECIIGSAFCLIIHNNDVRFAVNLDALSRSGVKVNP
                     DVLMLARKKNDG"
     gene            1065..2291
                     /gene="dgcN"
                     /locus_tag="J1Y83_003284"
     CDS             1065..2291
                     /gene="dgcN"
                     /locus_tag="J1Y83_003284"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcN"
                     /protein_id="HBA7631402.1"
                     /translation="MMDNDNSLHKRPTFKRALRNISMTSIFITMTLIWLLLSVTSVLT
                     LKQYAQKNLALTAATMTYSLEAAVVFADGPAATETLAALSQQGQFSTAEVRDKQQNIL
                     ASWHYTHKEPGDTFSNFISHWLFPAPIIQPIRHNGETIGEVRLTARDSSISHFIWFSL
                     AVLTGCILLASGIAITLTRHLHNGLVEALKNITDVVHDVRSNRNFSRRVSEERIAEFH
                     RFALDFNSLLDEMEEWQLRLQAKNAQLLRTALHDPLTGLANRAAFRSGINTLMNNSDA
                     RKTSALLFLDGDNFKYINDTWGHATGDRVLIEIAKRLAEFGGLRHKAYRLGGDEFAMV
                     LYDVQSESEVQQICSALTQIFNLPFDLHNGHQTTMTLSIGYAMTIEHASAENLQELAD
                     HNMYQAKHQRAEKLVR"
     gene            2307..2789
                     /locus_tag="J1Y83_003285"
     CDS             2307..2789
                     /locus_tag="J1Y83_003285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HBA7631403.1"
                     /translation="MIKHLLAPLIFTSLILTGCQSPQGKFTPEQVAAMQSYGFTESAG
                     DWSLGLSDAILFAKNDYKLLPESQQQIQTMAAKLASTGLTHARMDGHTDNYGEDSYNE
                     VLSLKRANVVADAWAMGGQIPRSNLTTQGLGKKYPIASNKTAQGRAENRRVAVVITAP
                     "
     gene            complement(2866..3213)
                     /gene="rplS"
                     /locus_tag="J1Y83_003286"
     CDS             complement(2866..3213)
                     /gene="rplS"
                     /locus_tag="J1Y83_003286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012768745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HBA7631404.1"
                     /translation="MSNIIKQLEQEQMKQDVPSFRPGDTVEVKVWVVEGSKKRLQAFE
                     GVVIAIRNRGLHSAFTVRKISNGEGVERVFQTHSPVVDSISVKRRGAVRKAKLYYLRE
                     RTGKAARIKERLN"
     gene            complement(3255..4022)
                     /gene="trmD"
                     /locus_tag="J1Y83_003287"
     CDS             complement(3255..4022)
                     /gene="trmD"
                     /locus_tag="J1Y83_003287"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HBA7631405.1"
                     /translation="MWIGIISLFPEMFRAITDYGVTGRAVKNGLLSIQSWSPRDFTHD
                     RHRTVDDRPYGGGPGMLMMVQPLRDAIHAAKAAAGEGAKVIYLSPQGRKLDQAGVSEL
                     ATNQKLILVCGRYEGIDERVIQTEIDEEWSIGDYVLSGGELPAMTLIDSVSRFIPGVL
                     GHEASATEDSFAEGLLDCPHYTRPEVLEGMEVPPVLLSGNHAEIRRWRLKQSLGRTWL
                     RRPELLENLALTEEQARLLAEFKTEHAQQQHKHDGMA"
     gene            complement(4053..4601)
                     /gene="rimM"
                     /locus_tag="J1Y83_003288"
     CDS             complement(4053..4601)
                     /gene="rimM"
                     /locus_tag="J1Y83_003288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000043333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HBA7631406.1"
                     /translation="MSKQLTAQAPVDPIVLGKMGSSYGIRGWLRVFSSTEDAESIFDY
                     QPWFIQKAGQWQQVQLESWKHHNQDMIIKLKGVDDRDAANLLTNCEIVVDSSQLPQLE
                     EGDYYWKDLMGCQVVTTEGYDLGKVVDMMETGSNDVLVIKANLKDAFGIKERLVPFLD
                     GQVIKKVDLTTRSIEVDWDPGF"
     gene            complement(4620..4868)
                     /gene="rpsP"
                     /locus_tag="J1Y83_003289"
     CDS             complement(4620..4868)
                     /gene="rpsP"
                     /locus_tag="J1Y83_003289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HBA7631407.1"
                     /translation="MVTIRLARHGAKKRPFYQVVVADSRNARNGRFIERVGFFNPIAS
                     EKEEGTRLDLDRIAHWVGQGATISDRVAALIKEVNKAA"
     gene            complement(5005..6366)
                     /gene="ffh"
                     /locus_tag="J1Y83_003290"
     CDS             complement(5005..6366)
                     /gene="ffh"
                     /locus_tag="J1Y83_003290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HBA7631408.1"
                     /translation="MFDNLTDRLSRTLRNISGRGRLTEDNVKDTLREVRMALLEADVA
                     LPVVREFINRVKEKAVGHEVNKSLTPGQEFVKIVRNELVAAMGEENQTLNLAAQPPAV
                     VLMAGLQGAGKTTSVGKLGKFLREKHKKKVLVVSADVYRPAAIKQLETLAEQVGVDFF
                     PSDVGQKPVDIVNAALKEAKLKFYDVLLVDTAGRLHVDEAMMDEIKQVHASINPVETL
                     FVVDAMTGQDAANTAKAFNEALPLTGVVLTKVDGDARGGAALSIRHITGKPIKFLGVG
                     EKTEALEPFHPDRIASRILGMGDVLSLIEDIESKVDRAQAEKLASKLKKGDGFDLNDF
                     LEQLRQMKNMGGMASLMGKLPGMGLIPDNVKSQMDDKVLVRMEAIINSMTMKERAKPE
                     IIKGSRKRRIAAGCGMQVQDVNRLLKQFDDMQRMMKKMKKGGMAKMMRSMKGMMPPGF
                     PGR"
     gene            6458..7324
                     /locus_tag="J1Y83_003291"
     CDS             6458..7324
                     /locus_tag="J1Y83_003291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311501.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YpjD"
                     /protein_id="HBA7631409.1"
                     /translation="MQRLEQRSPDAILLLFLIAQTVDITMPVFALLALVAYSVSLALI
                     VPGLLQKNGGWRRMAIISAVIALVCHAIALEARILPDGDSGQNLSLLNVGSLVSLMIC
                     TVMTIVASRNRGWLLLPIVYAFALINLALATFMPNEYITHLEATPGMLVHIGLSLFSY
                     ATLIIAALYALQLAWIDYQLKNKKLAFNQEMPPLMSIERKMFHITQIGVVLLTLTLCT
                     GLFYMHNLFSMENIDKAVLSIVAWFVYIVLLWGHYHEGWRGRRVVWFNVAGAVILTLA
                     YFGSRIFQQLIS"
     gene            7345..8631
                     /locus_tag="J1Y83_003292"
     CDS             7345..8631
                     /locus_tag="J1Y83_003292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026171.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HBA7631410.1"
                     /translation="MEHISTTTLIIILIIMVVISAYFSGSETGMMTLNRYRLRHMAKQ
                     GNRSAKRVEKLLRKPDRLISLVLIGNNLVNILASALGTIVGMRLYGDAGVAIATGVLT
                     FVVLVFAEVLPKTIAALYPEKVAYPSSFLLAPLQVLMMPLVWLLNAITRILMRMMGIK
                     TDIVVSGSLSKEELRTIVHESRSQISRRNQDMLLSVLDLEKMTVDDIMVPRSEIIGID
                     INDDWKSILRQLSHSPHGRIVLYRDSLDDAISMLRVREAWRLMSEKKEFTKETMLRAA
                     DEIYFVPEGTPLSTQLVKFQRNKKKVGLVVNEYGDIQGLVTVEDILEEIVGDFTTSMS
                     PTLAEEVTPQNDGSVIIDGTANVREINKAFNWHLPEDDARTVNGVILEALEEIPVAGT
                     RVRIGEYDIDILDVQDNMIKQVKVFPVKPLRESVAE"
     gene            complement(8686..9279)
                     /gene="grpE"
                     /locus_tag="J1Y83_003293"
     CDS             complement(8686..9279)
                     /gene="grpE"
                     /locus_tag="J1Y83_003293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HBA7631411.1"
                     /translation="MSSKEQKTPEGQAPEEIIMDQHEEIEAVEPEASAEQVDPRDEKI
                     ANLEAQLAEAQTRERDGILRVKAEMENLRRRTELDIEKAHKFALEKFINELLPVIDSL
                     DRALEVADKANPDMSAMVEGIELTLKSMLDVVRKFGVEVIAETNVPLDPNVHQAIAMV
                     ESDDVAPGNVLGIMQKGYTLNGRTIRAAMVTVAKAKA"
     gene            9402..10280
                     /gene="nadK"
                     /locus_tag="J1Y83_003294"
     CDS             9402..10280
                     /gene="nadK"
                     /locus_tag="J1Y83_003294"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) kinase"
                     /protein_id="HBA7631412.1"
                     /translation="MNNHFKCIGIVGHPRHPTALTTHEMLYRWLCTKGYEVIVEQQIA
                     HELQLKNVKTGTLAEIGQQADLAVVVGGDGNMLGAARTLARYDIKVIGINRGNLGFLT
                     DLDPDNAQQQLADVLEGHYISEKRFLLEAQVCQQDCQKRISTAINEVVLHPGKVAHMI
                     EFEVYIDEIFAFSQRSDGLIISTPTGSTAYSLSAGGPILTPSLDAITLVPMFPHTLSA
                     RPLVINSSSTIRLRFSHRRNDLEISCDSQIALPIQEGEDVLIRRCDYHLNLIHPKDYS
                     YFNTLSTKLGWSKKLF"
     gene            10366..12027
                     /gene="recN"
                     /locus_tag="J1Y83_003295"
     CDS             10366..12027
                     /gene="recN"
                     /locus_tag="J1Y83_003295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HBA7631413.1"
                     /translation="MLAQLTISNFAIVRELEIDFHSGMTVITGETGAGKSIAIDALGL
                     CLGGRAEADMVRTGAARADLCARFSLKDTPAALRWLEENQLEDGHECLLRRVISSDGR
                     SRGFINGTAVPLSQLRELGQLLIQIHGQHAHQLLTKPEHQKFLLDGYANETSQLQEMT
                     ARYHLWHQSCRDLAHHQQLSQERAARAELLQYQLKELNEFNPQPGEFEQIDEEYKRLA
                     NSGQLLTTSQNALALMADGEDANLQSQLYTAKQLVSELIGMDSKLSGVLDMLEEATIQ
                     IAEASDELRHYCDRLDLDPNRLFELEQRISKQISLARKHHVSPEALPQYYQSLLEEQQ
                     QLDDQADSQETLALAVTKHHQQALETARALHQQRQHYANELAQLITDSMHALSMPHGQ
                     FTIDVKFDEHHLGADGADRIEFRVTTNPGQPMQPIAKVASGGELSRIALAIQVITARK
                     METPALIFDEVDVGISGPTAAVVGKLLRQLGESTQVMCVTHLPQVAGCGHQHYFVSKE
                     TDGAMTETHMQSLDKKARLQELARLLGGSEVTRNTLANAKELLAA"
     gene            12176..12517
                     /gene="bamE"
                     /locus_tag="J1Y83_003296"
     CDS             12176..12517
                     /gene="bamE"
                     /locus_tag="J1Y83_003296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417107.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamE"
                     /protein_id="HBA7631414.1"
                     /translation="MRCKTLTAAAAVLLMLTAGCSTLERVVYRPDINQGNYLTANDVS
                     KIRVGMTQQQVAYALGTPLMSDPFGTNTWFYVFRQQPGHEGVTQQTLTLTFNSSGVLT
                     NIDNKPALSGN"
     gene            complement(12579..12869)
                     /locus_tag="J1Y83_003297"
     CDS             complement(12579..12869)
                     /locus_tag="J1Y83_003297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417108.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfH family protein"
                     /protein_id="HBA7631415.1"
                     /translation="MPGKIAVEVAYALPEKQYLQRVTLQEGATVEEAIRASGLLELRT
                     DIDLTKNKVGIYSRPAKLSDIVHDGDRVEIYRPLIADPKELRRQRAEKSANK"
     gene            complement(12859..13335)
                     /gene="ratA"
                     /locus_tag="J1Y83_003298"
     CDS             complement(12859..13335)
                     /gene="ratA"
                     /locus_tag="J1Y83_003298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin RatA"
                     /protein_id="HBA7631416.1"
                     /translation="MILFVGFLLMEIVMPQISRTALVPYSAEQMYQLVNDVQSYPQFL
                     PGCTGSRILESTPGQMTAAVDVSKAGISKTFTTRNQLTSNQSILMSLVDGPFKKLIGG
                     WKFTPLSQDACRIEFHLDFEFTNKLIELAFGRVFKELAANMVQAFTVRAKEVYSAR"
     gene            13467..13949
                     /gene="smpB"
                     /locus_tag="J1Y83_003299"
     CDS             13467..13949
                     /gene="smpB"
                     /locus_tag="J1Y83_003299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HBA7631417.1"
                     /translation="MTKKKAHKPGSATIALNKRARHEYFIEEEFEAGLALQGWEVKSL
                     RAGKANISDSYVLLRDGEAFLFGANITPMAVASTHVVCDPTRTRKLLLNQRELDSLYG
                     RVNREGYTVVALSLYWKNAWCKVKIGVAKGKKQHDKRSDIKEREWQVDKARIMKNAHR
                     "
     gene            14163..14525
                     /gene="ssrA"
                     /locus_tag="J1Y83_003300"
     tmRNA           14163..14525
                     /gene="ssrA"
                     /locus_tag="J1Y83_003300"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
BASE COUNT         3859 a         3914 c         3510 g         3744 t
ORIGIN      
        1 agctttaaaa acaatccgct atgctttgaa aaaggagaaa gaaacgatga aaaaatttat
       61 cgcccccttg ttggctttac tggttagcgg atgtcagatt gatccttata ctcacgcgcc
      121 aaccttgacc agcaccgact ggtatgatgt tggtatggaa gatgcgatat cgggcagcgc
      181 cattaaagat gacgatgcat ttagcgattc acaggcggat cgcggtctat accttaaagg
      241 atatgccgaa gggcaaaaga aaacttgcca gaccgatttt acttatgccc ggggactttc
      301 tggtaaaagc tttcctgcca gctgcaataa tgttgaaaat gccagccaac tgcatgaagt
      361 atggcgaaaa ggggctgatg aaaatgccag cacaatacga ttaaattaat taccccccaa
      421 aatcaattaa gcatgagatt ttactcatta tgttttattt ctcacattga tgacgaccgc
      481 atgaaaggat gatattttaa tgagcattct gaaaaaatat cgatgcattt cgagcgaaga
      541 tggtgaggat ccctgaatgc gtttttctca ccgactgttc ttactgctga ttctcctgct
      601 gacgggagca ccaatactcg ctcaggaacc ttctgacgtt gcgaaaaacg tgcgtatgat
      661 ggtttccggt attgtcagtt acacccgctg gcctgggcta tcagggccac caaagttatg
      721 tatcttttca tcttcacgtt ttagtaccgc cttacaagaa aatgctgcta catctttacc
      781 ttatctgcct gtcatcattc atactcaaca agaggcgata atttcaggct gcaatggttt
      841 ttattttggc aacgagtcac cgacattcca gatggaatta accgaacaat atccgtcaaa
      901 ggcgttatta ttaattgccg aacaaaatac cgagtgcatt attggtagcg ccttttgtct
      961 gatcatccac aataatgacg tcagatttgc cgtaaacctg gatgccttat cgcgtagcgg
     1021 tgtaaaggtc aacccggatg tcttaatgct cgcacggaag aaaaatgatg gataacgata
     1081 attctcttca taagcgcccc acgtttaaaa gagcattacg caacatcagc atgaccagca
     1141 tatttatcac tatgacgctg atctggctgc tgctttccgt gacgtcagtg ctaaccctga
     1201 aacaatacgc gcaaaaaaac ctggcactga cagcagcaac aatgacttac agtctggaag
     1261 cagctgtcgt ttttgccgat ggccctgcag caactgaaac actggcagcg ctgagccagc
     1321 aaggccaatt ttcaactgca gaagtgcgtg ataagcagca aaatattctg gcatcctggc
     1381 attacaccca taaggaacca ggcgatactt tcagcaattt cataagccac tggctcttcc
     1441 ctgcccccat cattcagccg attcgccaca atggtgaaac cattggcgaa gtacgcttaa
     1501 ctgctcgcga cagttcaatc agccatttta tctggttttc gctcgctgtc ctgaccggtt
     1561 gtattctgct ggcatcaggc atcgcaatta ccctcacccg ccatttgcac aatggcctgg
     1621 tggaagcgct gaaaaatatc accgatgtcg tacatgatgt gcgttctaac cgcaattttt
     1681 cccgacgagt ttcggaagaa cgtatcgctg agtttcaccg cttcgctctc gacttcaaca
     1741 gtctgctgga tgaaatggaa gagtggcagc ttcgtttaca ggccaaaaat gcgcagcttc
     1801 tacgtaccgc gctacatgac ccattaaccg ggctggctaa ccgcgcagcg tttcgtagcg
     1861 gcatcaacac gttgatgaac aattccgatg cccgaaaaac gtcggcgtta ctatttcttg
     1921 atggcgataa tttcaaatat atcaatgata cctggggtca tgcgacgggc gatagagtct
     1981 tgattgaaat agcaaaacgg ttagctgaat ttggcgggct gcgacataaa gcataccgcc
     2041 tgggcggcga tgaattcgct atggtgctct atgatgtaca gtcggaatct gaagtgcagc
     2101 agatatgctc agcactgaca caaattttta atctcccgtt tgatcttcat aatggccatc
     2161 agactaccat gacattaagc attggttacg cgatgactat tgagcacgcc tctgcggaaa
     2221 atttacaaga gcttgccgat cacaatatgt atcaggccaa acaccagcgt gccgaaaagc
     2281 tggtgagata acaaggatat atcgatatga taaagcacct gctagcaccc ctgattttca
     2341 cctcgctaat acttactggc tgccagtccc ctcagggaaa gtttactcct gagcaagtcg
     2401 ccgctatgca atcttatgga tttactgaat ccgccggcga ctggtcgctg ggcttatcag
     2461 atgccattct gttcgcaaaa aatgactaca aattgctccc ggaaagccag caacaaatcc
     2521 aaaccatggc agccaaattg gcctcgacag ggctaacaca tgcccgtatg gatggacaca
     2581 ccgacaacta tggtgaagac agttacaacg aagtcttatc attaaaacgg gcgaatgtcg
     2641 tggccgatgc atgggctatg ggtggacaaa ttccgcgcag caatctcacc acacagggtt
     2701 taggaaaaaa atatcccata gccagtaaca agaccgccca gggccgcgcc gagaaccgcc
     2761 gcgtcgcagt ggtgattaca gccccttaaa acacgagcaa acagacaaaa aaagggccag
     2821 ccaattggcc agcccttctt aacaggatgt cgcttaagcg aaatcttagt taagacgctc
     2881 tttgatacga gcagccttac cagtacgctc acgcaggtag tacagtttag ctttacgaac
     2941 agcaccacga cgtttgacag aaatgctgtc aactaccgga gagtgagtct ggaagacacg
     3001 ctcaacgcct tcgccgttgg aaattttacg aacagtgaat gcagagtgca gaccgcggtt
     3061 acgaatagcg ataaccacgc cctcgaatgc ctgcagacgt tttttggaac cttcaacaac
     3121 ccatactttc acttccacgg tatcacccgg acggaaggaa ggtacgtcct gcttcatctg
     3181 ctcttgttca agttgcttaa taatgttgct cataatttaa tctcttatcc tgggtaaact
     3241 gatatctcgg gggcttacgc catcccatca tgtttatgtt gctgttgtgc gtgttccgtt
     3301 ttgaactccg ccagcaacct tgcttgctct tcagtcagag ccaggttttc cagaagttca
     3361 ggtcttctaa gccaggtacg gcccagcgac tgtttcaaac gccagcgacg tatctcggca
     3421 tggttgcccg acagtaacac tggcggaact tccatccctt ctaacacctc aggccgcgta
     3481 tagtgcgggc aatccagcaa tccttcagca aaggaatctt ccgttgccga ggcttcatgt
     3541 cccagtactc ccggaataaa ccgggaaacg gagtcaatca gcgtcattgc tggtaactca
     3601 ccaccactga gaacgtaatc gccgattgac cattcttcgt caatttcggt ttggatcacg
     3661 cgctcatcta taccttcgta gcgaccacac accagaatca atttttgatt cgttgccagt
     3721 tcgctgacgc ccgcttgatc aagcttgcgt ccctgtggtg acagataaat cacctttgcg
     3781 ccttcacccg ccgcggcttt tgctgcatga atggcgtccc gcaagggttg caccatcatt
     3841 aacatccccg gtccgccgcc gtaaggacga tcgtccacgg tacggtgccg gtcatgcgtg
     3901 aagtcgcgag gactccagct ctggatgctc agcaggccat ttttaactgc ccggccagtt
     3961 accccgtaat cggtaattgc gcggaacatt tcaggaaaca ggctaattat gccaatccac
     4021 atagcgccgt cttttaccgt ttatccggtg gtttaaaaac caggatccca atctacttcg
     4081 attgaacgtg tagtgagatc gactttcttg ataacctgcc catcgaggaa cggtacgaga
     4141 cgttccttga taccaaacgc atctttcagg tttgccttaa tgacgagaac gtcattagat
     4201 ccggtttcca tcatatctac gactttaccg agatcgtagc cttcagtggt tactacctgg
     4261 cagcccatca ggtctttcca gtagtagtcc ccctcttcaa gctgaggcaa ctgcgatgaa
     4321 tccacgacaa tttcacaatt cgtcagcagg ttcgccgcat cacgatcgtc aacgcctttc
     4381 agcttgatga tcatgtcctg attgtggtgc ttccagcttt ccagctggac ttgctgccac
     4441 tgacccgcct tctggataaa ccagggctga tagtcaaaaa tgctttcggc gtcttcggtg
     4501 gaagaaaaca ctctgagcca cccacgaata ccgtaagacg accccatttt tcccaaaacg
     4561 atgggatcaa caggtgcttg cgcggtgagt tgtttgctca tcatgaccac cgtgacagat
     4621 taagctgctt tgtttacttc tttgatcagc gcagcaacgc gatcagaaat agttgcgccc
     4681 tggccaaccc agtgagcgat gcgatccaga tccaggcgag tgccttcttc tttttcgcta
     4741 gcgattgggt tgaagaaacc aacgcgctcg atgaagcgac cgttgcgtgc attacggctg
     4801 tcagcgacaa caacctggta gaacggacgc tttttagcgc cgtgacgtgc taaacgaata
     4861 gttaccataa catcctcttg tgtgaataaa acaaccggac cccatcgagg aacggagtcc
     4921 ggtgtcatat taaaagcccg aaaattttac tgatttctgg agaaaatgca atcagcagtt
     4981 aataactctg tcgcataagg tcatttagcg accaggaaat cccggcggca tcatcccttt
     5041 catgcttctc atcatcttcg ccatcccgcc cttcttcatt ttcttcatca tgcgctgcat
     5101 gtcgtcgaac tgtttcagaa gacggttaac gtcctgcacc tgcataccgc aaccggcagc
     5161 gatacgacgc ttacgcgaac ctttgatgat ttctggctta gcgcgctctt tcatcgtcat
     5221 cgagttgatg atggcttcca tacgcaccag cactttatcg tccatctgtg acttgacgtt
     5281 atccgggatc agccccatgc ccggcagctt gcccatcagg ctagccatgc cgcccatatt
     5341 tttcatctgg cgcagctgct caagaaagtc gttgagatcg aagccgtcgc cttttttcag
     5401 cttgctggct aatttctctg cctgcgcgcg gtcaaccttg ctttcgatat cttcgatcag
     5461 tgacagtacg tcgcccatgc cgagaatacg ggaggcaata cggtccggat ggaatggctc
     5521 cagcgcctcg gttttctcgc caacaccgag gaacttgatc ggtttgccag tgatgtgacg
     5581 aatagagagc gccgcaccgc cgcgggcatc gccgtccact ttggtcaaca ctacaccggt
     5641 aagcggtaac gcttcattga atgcttttgc cgtattggcc gcatcctgac cggtcatggc
     5701 atcgaccaca aacagggttt caaccggatt aatcgacgca tggacttgtt tgatctcgtc
     5761 catcatcgct tcgtcaacgt gcagacgacc ggcagtatcc accagcagca cgtcgtagaa
     5821 tttcagtttg gcttctttca gcgccgcgtt aacgatatct accggcttct gaccaacatc
     5881 agaagggaag aaatcaacgc ccacctgctc tgccagcgtc tcaagctgtt tgattgccgc
     5941 cgggcgatag acgtcggcag aaaccaccag tactttcttc ttgtgcttct cgcgcaggaa
     6001 cttaccaagt ttaccaacgc tggttgtttt accggcacct tgcaggcccg ccatcagcac
     6061 gaccgcaggc ggttgcgcag ccaggttcag ggtctggttc tcttcgccca tcgccgcaac
     6121 cagttcgtta cggacaattt tgacgaactc ctgccccggc gtcaggctct tattaacttc
     6181 atgaccaacc gctttctctt ttacgcgatt gataaactca cgcactaccg gcagagcgac
     6241 gtccgcctcc agcagcgcca tgcgcacttc gcgcagcgta tcttttacgt tgtcttcagt
     6301 gaggcgtcca cggccactga tattgcgcag cgtgcgcgac aaacgatcgg ttaaattatc
     6361 aaacattgtc tctcgcctgg ggtggaaacg gttggccgca atcgcgacac atcatcagta
     6421 ttttgccgca gtataacatg aaggcgtctt tgttgttatg caacggttgg agcagcgttc
     6481 acctgacgct atactgcttc tctttcttat tgctcaaact gtcgacatta ctatgcccgt
     6541 ttttgctctg ctcgcgcttg tcgcctactc cgtcagtctt gcgctgattg ttcccggtct
     6601 gctgcaaaaa aacggcggct ggcggcgcat ggctattatt tctgcggtca tcgcgctggt
     6661 ctgccacgca atcgctctgg aagcccgcat cctgcccgac ggtgatagcg gacaaaacct
     6721 cagcctgctg aacgttggtt cattggtcag tttgatgatc tgtacggtaa tgaccattgt
     6781 ggcttctcgc aatcgtggct ggttgttgct acccattgtc tatgcctttg cgcttatcaa
     6841 cctggcgctg gcgaccttca tgcccaatga atacatcacc catctggaag caacgccggg
     6901 gatgctggtg cacattggct tatcgctttt ttcctatgcc acgctaatta tcgctgccct
     6961 gtacgcgctg caactggcgt ggattgatta ccaactgaag aacaagaagc tggcgtttaa
     7021 ccaggaaatg cctccattga tgagtatcga gcgtaaaatg ttccatatca cgcagattgg
     7081 cgtggtgctg ctaacgctca ctctttgcac tggcctgttc tacatgcaca acttatttag
     7141 catggaaaat atcgacaagg ccgtgctctc tatcgtggcg tggtttgtct atattgtgct
     7201 gctgtgggga cattatcatg aaggatggcg tggacgccgc gtcgtctggt ttaacgttgc
     7261 gggtgcagtc attctgacac tggcctactt cggcagccga atttttcagc agttaatcag
     7321 ctaaacccag aaaaggagtt tcccctggaa cacatttcta ctactacgtt gatcattatt
     7381 ctgatcatca tggtggtcat ttcagcctat ttttccggtt cagaaactgg aatgatgaca
     7441 ctcaaccgct atcgcctgag acacatggcg aagcaaggta atcgctcggc aaaacgcgtc
     7501 gaaaaattac tgcgcaagcc agaccgcctg ataagcctgg tgttaatcgg caataatctg
     7561 gtcaatatcc ttgcttccgc actcggcact attgtcggga tgcgcttgta tggcgatgct
     7621 ggcgtggcaa ttgcgactgg cgtgctgacc ttcgtagtgc tggtttttgc tgaagtattg
     7681 ccgaaaacca ttgccgcact atacccggaa aaagtcgcct atccgagtag ttttctactg
     7741 gcgccgctac aagtcctgat gatgccgcta gtctggttac taaatgccat cacccgaatt
     7801 ttgatgcgta tgatgggtat taaaaccgat atcgtagtca gcggctcttt gagtaaagaa
     7861 gagttgcgca ccatcgtgca cgaatcacgc tcacaaattt cccgtcgcaa tcaggatatg
     7921 ctgctgtcgg tgctcgatct ggaaaagatg accgtcgatg acatcatggt gccgcgcagt
     7981 gaaattatcg gtattgatat caacgatgac tggaaatcga ttctgcgcca actctcccac
     8041 tcacctcacg ggcgcatcgt gctctaccgt gattcgctgg acgacgccat cagtatgctg
     8101 cgtgtgcgtg aagcctggcg actcatgtcg gagaaaaaag agttcaccaa agaaaccatg
     8161 ctacgcgccg cggacgagat ctattttgtc ccggagggta cgccgctcag cacacagttg
     8221 gtgaagtttc agcgcaacaa aaagaaagtc ggactggtcg tcaacgagta tggagacatt
     8281 cagggactgg tgacggtcga agatattctc gaagagattg tcggcgattt caccacgtcg
     8341 atgtcgccaa cacttgccga agaggtcacg ccgcaaaacg acggttcggt gattattgat
     8401 ggcactgcca acgtgcgaga aattaacaaa gcctttaact ggcatctacc ggaagatgat
     8461 gctcgtactg ttaacggcgt cattctggaa gcgctggagg agatcccggt agcaggcacc
     8521 cgcgtgcgta taggcgagta cgatatcgat attctcgacg tacaggacaa tatgattaag
     8581 caggtaaaag tttttcctgt aaaaccgctg cgcgagagtg tggcggagta acgaaaacgg
     8641 cccggcattc gcatgctggg ccgtgattta ttacgaaagc agaaattaag cttttgcttt
     8701 cgctacagtc accatcgccg cacgaatcgt acgaccgttc agcgtataac ctttctgcat
     8761 aatgcccagt acgttacctg gcgcaacgtc atcagattcc accattgcga tggcctgatg
     8821 cacatttggg tccagtggga cgttggtttc ggcgatcact tcaacgccaa acttacgcac
     8881 aacatccagc atcgacttca gcgtcagctc aataccttca accatcgcgg acatatccgg
     8941 gttagcttta tcagccactt ccagcgcacg atccaggcta tcaatcaccg gcagcaattc
     9001 gttgatgaat ttctccagcg cgaatttgtg ggctttttcg atatccagtt cagtacgacg
     9061 acgcaggttt tccatttcgg cttttacgcg caaaatgccg tcacgttcac gggtctgggc
     9121 ttcagccagc tgagcttcga gattcgcaat tttttcatcg cgcggatcca cctgctcagc
     9181 agaagcttct ggctcaactg cctcaatctc ttcgtgctga tccatgataa tttcttccgg
     9241 ggcttgcccc tcaggcgttt tctgttcttt actactcatg aatttctccg cgtttttttc
     9301 gcattcatct cgctaacttc gcttattatg gggatcagtt tcagggtttc aagggaagca
     9361 ctcacattgt catcaatctt cgcaacaagg acctcggaaa aatgaataat catttcaagt
     9421 gtattggcat tgtgggacac ccacggcacc ccactgcact gacaacacat gaaatgctct
     9481 accgctggct gtgcacaaaa ggttacgagg tcatcgttga gcaacaaatc gctcacgaac
     9541 tgcaactgaa gaatgtgaaa actggcacgc tcgcggagat tgggcaacag gctgatctcg
     9601 cggttgtcgt tggcggcgac ggtaatatgc tgggcgcggc gcgcacgctc gcccgctacg
     9661 atattaaagt tattggaatc aaccgtggca acctgggttt cctgactgac cttgatcccg
     9721 ataacgccca gcaacaatta gccgatgtgc tggaaggcca ctacatcagc gagaaacgtt
     9781 ttttgctgga agcgcaagtc tgtcagcaag attgccagaa acgcatcagc acggcgatta
     9841 acgaagtggt acttcatcct ggcaaagtag cgcatatgat tgagttcgaa gtgtatatcg
     9901 acgagatctt tgcgttttcg cagcgttccg atggactgat tatttcgacg ccaacaggct
     9961 ccaccgcata ttccctctct gcgggcggtc ctattctgac accctctctg gatgcgatta
    10021 ccctggtgcc catgttcccg catacgttgt cagcacgacc actggtcata aacagcagca
    10081 gcacgatccg tctgcgtttt tcgcatcgcc gtaacgacct ggaaatcagt tgcgacagcc
    10141 agatagcact gccgattcag gaaggtgaag atgtcctgat tcgtcgctgt gattaccatc
    10201 tgaatctgat tcatccgaaa gattacagtt atttcaatac attaagcacc aagctcggct
    10261 ggtcaaaaaa attattctaa ttttacgcca gcctctttac tgtatataaa accagtttat
    10321 actgtacaca ataacagtaa tggtttttca tacaggaaaa caactatgtt ggcacaactg
    10381 accatcagca actttgctat cgttcgtgag cttgagattg attttcatag cggcatgacc
    10441 gtaataactg gcgagaccgg cgcgggtaaa tctattgcaa tagatgccct cggcctttgt
    10501 ctcggtggtc gcgctgaagc cgacatggtg cgtaccggcg ctgctcgtgc cgacctgtgc
    10561 gcccgttttt ctctgaaaga tacgccagcg gccctgcgtt ggctggaaga aaaccagctt
    10621 gaagacgggc atgaatgttt gcttcgtcgc gtaatcagca gcgatggtcg ctcccgtggt
    10681 ttcatcaacg gtacagctgt tcctctgtca caactgcgcg aactgggtca gttgctgatt
    10741 cagatccatg gtcagcacgc tcatcagtta ctcaccaaac ccgagcacca aaaattcctg
    10801 cttgatggct atgccaatga aacctctcag ctccaggaaa tgaccgcacg ttatcatttg
    10861 tggcatcaaa gctgccgtga cctcgcgcat catcaacagt taagtcagga acgcgccgct
    10921 cgtgcggagc tgctgcaata ccaattaaaa gaacttaacg aatttaatcc gcagcctgga
    10981 gagtttgagc aaatcgacga agagtacaaa cgtctggcga acagcggtca attgctgacc
    11041 accagccaga atgcattggc attaatggct gacggtgaag acgcaaacct gcaaagtcaa
    11101 ctttacacgg ctaaacaact ggtgagcgaa ttgattggca tggacagcaa actgtccggc
    11161 gtacttgata tgctggaaga ggctaccatc cagattgctg aagccagtga tgaactgcgc
    11221 cactactgcg atcgtctgga tctcgatccc aaccgactgt ttgaacttga acagcgcatc
    11281 tcaaaacaga tttcgctggc acgtaaacat cacgtcagcc ctgaggcatt gccacagtat
    11341 taccagtcgc tactggaaga acagcagcaa ctggacgatc aggccgactc acaagaaacg
    11401 ctcgcgctgg cggtaacgaa acatcatcag caggcactgg aaacggcgcg cgcgttacac
    11461 cagcaacgcc agcattatgc aaatgagttg gcacagctga tcaccgatag tatgcatgca
    11521 ctctctatgc cacatgggca gtttacgatc gatgttaaat ttgacgagca tcacctgggc
    11581 gctgacggcg ctgatcgtat tgagtttcgg gtaaccacca acccaggtca gccaatgcag
    11641 cctattgcca aagtcgcatc cggtggtgag ttgtcccgca tcgcattggc aatccaggtc
    11701 attaccgcgc gtaaaatgga aaccccggca ctgatttttg atgaagtgga tgtagggatt
    11761 agcggcccaa cagcggcagt tgtcggcaaa ctgctacgtc aacttggcga atcaactcag
    11821 gtgatgtgtg ttacccacct gccacaagtc gcgggatgtg gtcatcaaca ctattttgtc
    11881 agcaaagaaa ctgatggtgc gatgacagaa acgcatatgc aatccctgga taaaaaagcg
    11941 cggttacaag agttggcgcg cctgcttggt ggcagtgaag tcacacgtaa cacactggcg
    12001 aatgcgaaag aactgcttgc ggcgtaaact tttttcctgc ttcacggtca gagtaaacag
    12061 caaaacgccg taagaccgga aagcaaaagg ttttaaagtg atgaaaggtc tattatcatc
    12121 ggcatattac agatgagcca cgtactgctc gggcccgaaa aggaatcaaa tcactatgcg
    12181 ctgtaaaacg ctgactgctg cagcagcagt actattgatg ttgaccgcag gctgttccac
    12241 tctggagcga gtggtttacc gtcctgacat caaccagggg aactatctga ccgctaacga
    12301 cgtatccaaa atacgtgttg gcatgacgca acaacaagtt gcgtacgcat tgggtacacc
    12361 gctgatgtcc gatccatttg gtacaaatac ctggttctat gtcttccgcc agcaacctgg
    12421 tcatgaaggt gtaacgcagc aaacgctgac gctgaccttt aacagtagcg gtgtgttgac
    12481 caacattgat aacaaacctg cgctgagtgg taactaataa taaagttgct ctcaaagacg
    12541 ttaaaaaagg tgctcaatga gcaccttttt tctgtctgtt atttattcgc tgatttttct
    12601 gctcgttgcc tgcgaagctc tttcggatcg gcaatgagag gacgataaat ctccacccga
    12661 tcgccatcat gcacaatatc gctgagtttt gccggacggc tgtaaatgcc gactttgttt
    12721 ttagttaaat cgatatcggt acgcaattcc agcaaaccac tggcgcgaat agcttcttca
    12781 accgtcgcgc cctcctgcaa cgtcacgcgc tgcaggtact gcttctcagg tagcgcataa
    12841 gccacctcaa cggcaatttt acctggcact gtagacctct tttgcacgaa cagtaaaagc
    12901 ctgcaccata ttagctgcca gctctttaaa cacacgacca aaggcgagtt caatcaactt
    12961 attggtaaac tcaaagtcga gatgaaattc aatacggcac gcgtcctggc tcaacggcgt
    13021 aaacttccaa ccgccgatca atttcttaaa cggtccatca accagactca tgagaatact
    13081 ttgattactg gtcaactggt tgcgggttgt aaacgttttg ctgatcccag ccttagacac
    13141 atctaccgca gcagtcatct gcccaggagt ggactccaga atccgacttc cggtacaacc
    13201 cggcaaaaac tgaggataag actgaacgtc attcactaac tgatacattt gctccgcgct
    13261 gtagggcacc agtgcggtcc ggctaatctg aggcataaca atttccatca acaaaaatcc
    13321 aacaaataat atcatttatc ctgctgtaaa aaaaacgcta tcccggcgct gggtaacatc
    13381 gggttcatgc taagatagag ccttgtcccc cgcaggattg atatggggtg ttttcgattt
    13441 cagattaccg atgattcacg acgcttatga cgaagaaaaa agcacataaa cctggttcag
    13501 cgaccatcgc gcttaacaag cgcgcccgtc acgaatactt tatcgaagaa gagttcgaag
    13561 cgggacttgc cctgcaaggc tgggaggtta aatccctgcg tgcaggaaaa gccaatatca
    13621 gcgacagcta cgtccttctg cgtgacggag aggcatttct gtttggcgct aacatcacgc
    13681 caatggctgt ggcctccacg catgtggtgt gcgatcctac ccgtacccgc aagttacttc
    13741 tcaaccagcg cgaactggac tcattgtacg gtcgcgtcaa tcgagaaggc tataccgtag
    13801 tggcgctctc cctgtactgg aaaaatgcct ggtgcaaagt gaaaatcggc gtcgccaaag
    13861 gtaaaaaaca gcacgataaa cgttcagata tcaaagaacg cgaatggcag gtggataaag
    13921 cgcgtatcat gaaaaacgcc catcgttaaa cctatataag cgatactaat caaatcctca
    13981 ccgcgcctcc ctatccggtg gcgcgaatga acatcttatc gactgtcaaa tctgacacag
    14041 atgtagccat cccattgctt aatcgaaaaa aatcatacta tgcaggcgct aattccagga
    14101 tgataacgcc ttttgggcta gtcatgctgc tcataaatct ggtatactta ccgttacaca
    14161 ttggggctga ttctggattc gacgggattt gcgaaaccca aggtgcatgc cgaggggcgg
    14221 ttggcctcgt aaaaagccgc aaaaaatagt cgcaaacgac gaaaactacg ctttagcagc
    14281 ttaataacct gcttagagcc ctctctccct agcctccgct cttaggacgg ggatcaagag
    14341 aggtcaaacc caaaagagat cgcgtggaag ccctgcctgg ggttgaagcg ttaaaactta
    14401 atcaggctag tttgttagtg gcgtgtccgt ccgcagctgg caagcgaatg taaagactga
    14461 ctaagcatgt agtaccgagg atgtaggaat ttcggacgcg ggttcaactc ccgccagctc
    14521 caccaatcat gattggacgg tgtaaggact acaccaacaa aaacaggaag ttataagtct
    14581 cagcagaaca ccgaccagac ggtgaggaga caaaaaagga tacgcaacgg agtcgcgact
    14641 ccctatgaca ttaaagcccg catcagcggg cttttttatt ggtcctgacc cgccctgaat
    14701 agaattcatc caattacagt ccagacatat acagtatccc tcagctcagt atcagcttct
    14761 cgacaaatgg tcactgttat ttacccgttg ccagaaagag aataatttgc ccccatagcc
    14821 agccttcccg gaatctgtat attcattaat attgttatcg attccatcaa gtaacatagg
    14881 ttggatgcga ttgatcttac tcacattagt cgacacggaa ctcagtaagt tgaactctga
    14941 aaaagcagtt taaatagcat ttaaacaaat gccaccagtt cgaaaaagag tataagcaca
    15001 cttaatatat tgttttaatt attcatt
//
LOCUS       DADSYM010000122        14990 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2415
            SAMN08039579-rid11723173.denovo.123, whole genome shotgun sequence.
ACCESSION   DADSYM010000122 DADSYM010000000
VERSION     DADSYM010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039579
            Sequence Read Archive: SRR6362533
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14990)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14990)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980162.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 03:39:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,484
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 4,347
            CDSs (with protein)               :: 4,347
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 43 of 85
            Pseudo Genes (incomplete)         :: 37 of 85
            Pseudo Genes (internal stop)      :: 19 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14990
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039579-rid11723173.denovo.123"
                     /strain="LGE2415"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8302 N 95.9628 W"
                     /collection_date="2015-08-24"
     gene            complement(21..2300)
                     /gene="maeB"
                     /locus_tag="J1Y83_003301"
     CDS             complement(21..2300)
                     /gene="maeB"
                     /locus_tag="J1Y83_003301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxaloacetate-decarboxylating
                     malate dehydrogenase"
                     /protein_id="HBA7631418.1"
                     /translation="MDDQLKQSALDFHEFPVPGKIQVSPTKPLATQRDLALAYSPGVA
                     APCLEIEKDPLKAYKYTARGNLVAVISNGTAVLGLGNIGALAGKPVMEGKGVLFKKFA
                     GIDVFDIEVDELDPDKFIEVVAALEPTFGGINLEDIKAPECFYIEQKLRERMNIPVFH
                     DDQHGTAIISTAAILNGLRVVEKNISDVRMVVSGAGAAAIACMNLLVALGLQKHNIVV
                     CDSKGVIYQGREPNMAETKAAYAVVDDGKRTLDDVIEGADIFLGCSGPKVLTQEMVKK
                     MARAPMILALANPEPEILPPLAKEVRPDAIICTGRSDYPNQVNNVLCFPFIFRGALDV
                     GATAINEEMKLAAVRAIAELAHAEQSEVVASAYGDQDLSFGPEYIIPKPFDPRLIVKI
                     APAVAKAAMESGVATRPIADFDVYIDKLTEFVYKTNLFMKPIFSQARKAPKRVVLPEG
                     EEARVLHATQELVTLGLAKPILIGRPNVIEMRIQKLGLQIKAGVDFEIVNNESDPRFK
                     EYWTEYFQIMKRRGVTQEQAQRALISNPTVIGAIMVQRGEADAMICGTVGDYHEHFSV
                     VKNVFGYRDGVHTAGAMNALLLPSGNTFIADTYVNDEPDAEELAEITLMAAETVRRFG
                     IEPRVALLSHSNFGSSDCPSSSKMRQALELVRERAPELMIDGEMHGDAALVEAIRNDR
                     MPDSPLKGSANILVMPNMEAARISYNLLRVSSSEGVTVGPVLMGVAKPVHVLTPIASV
                     RRIVNMVALAVVEAQTQPL"
     gene            2589..3539
                     /locus_tag="J1Y83_003302"
     CDS             2589..3539
                     /locus_tag="J1Y83_003302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase A"
                     /protein_id="HBA7631419.1"
                     /translation="MNELDGIKQFTTVVADSGDIESIRHYHPQDATTNPSLLLKAAGL
                     SQYEHLIDDAIAWGKKNGKTQEQQVVAACDKLAVNFGAEILKIVPGRVSTEVDARLSF
                     DKEKSIEKARHLVDLYQQQGVEKSRILIKLASTWEGIRAAEELEKEGINCNLTLLFSF
                     AQARACAEAGVFLISPFVGRIYDWYQARKPMDPYVVEEDPGVKSVRNIYDYYKQHHYE
                     TIVMGASFRRTEQILALTGCDRLTIAPNLLKELQEKVSPVVRKLIPPSQTFPRPAPMS
                     EAEFRWEHNQDAMAVEKLSEGIRLFAVDQRKLEDLLAAKL"
     gene            3559..5562
                     /gene="tkt"
                     /locus_tag="J1Y83_003303"
     CDS             3559..5562
                     /gene="tkt"
                     /locus_tag="J1Y83_003303"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HBA7631420.1"
                     /translation="MSRKDLANAIRALSMDAVQKANSGHPGAPMGMADIAEVLWNDFL
                     KHNPTDPTWYDRDRFILSNGHASMLLYSLLHLTGYDLPLEELKNFRQLHSKTPGHPEI
                     GYTPGVETTTGPLGQGLANAVGLAIAERTLAAQFNQPDHEIVDHFTYVFMGDGCLMEG
                     ISHEVCSLAGTLGLGKLIGFYDHNGISIDGETEGWFTDDTAKRFEAYHWHVVHEIDGH
                     DPQAVKEAILEAQSVKDKPSLIICRTVIGFGSPNKAGKEEAHGAPLGEEEVALARQKL
                     GWHHPPFEIPKEIYHAWDAREKGEKAQQSWNEKFAAYKKAHPQLAEEFTRRMSGGLPK
                     DWEKTTQKYINELQANPAKIATRKASQNTLNAYGPMLPELLGGSADLAPSNLTIWKGS
                     VSLKEDPAGNYIHYGVREFGMTAIANGIAHHGGFVPYTATFLMFVEYARNAARMAALM
                     KARQIMVYTHDSIGLGEDGPTHQAVEQLASLRLTPNFSTWRPCDQVEAAVGWKLAVER
                     HNGPTALILSRQNLAQVERTPDQVKEIARGGYVLKDSGGKPDIILIATGSEMEITLQA
                     AEKLAGEGRNVRVVSLPSTDIFDAQDEEYRESVLPSNVSARVAVEAGIADYWYKYVGL
                     KGAIVGMTGYGESAPADKLFPFFGFTAENIVAKAHKVLGVKGA"
     gene            complement(5639..6682)
                     /locus_tag="J1Y83_003304"
     CDS             complement(5639..6682)
                     /locus_tag="J1Y83_003304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1176 domain-containing protein"
                     /protein_id="HBA7631421.1"
                     /translation="MRYRIFLLFFFVLLPMSLVWAAPAQRAFSDWQVTCNNQNFCVAR
                     NTGDHNGLVMTLSRSAGAHTDAVLRIERGGLKSPDASEGEIAPRLLLDGEPLALSGDK
                     WRISPWLLVTDDTATITAFLQMIQEGRAITLRDGNQTISLSGLKAALLFIDAQQKRVG
                     SETAWIKKGDEPPLSVPPAPALKEVAVVNPTPTPLSREERNDLLDYGNWRMNGLRCSL
                     DPLRREVNVTALTDDKALMMISCEAGAYNTIDLAWIVSRKKPLASRPVRLRLPFNNGQ
                     ETNELELMNATFDEKSRELVTLAKGRGLSDCGIQARWRFDGQRFRLVRYAAEPTCDNW
                     HGPDAWPTLWITR"
     gene            complement(6808..7383)
                     /gene="nudK"
                     /locus_tag="J1Y83_003305"
     CDS             complement(6808..7383)
                     /gene="nudK"
                     /locus_tag="J1Y83_003305"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose pyrophosphatase NudK"
                     /protein_id="HBA7631422.1"
                     /translation="MTQQITLVKDKILSDNYFTLHNITYDLTRKDGEVIRHKREVYDR
                     GNGATILLYNAKKKTVVLIRQFRVATWVNGNESGQLIETCAGLLDNDEPEVCIRKEAI
                     EETGYEVGEVRKLFELYMSPGGVTELIHFFIAEYSDSQRANAGGGVEDEDIEVLELPF
                     SQALEMIKTGEIRDGKTVLLLNYLQMSHLMD"
     gene            complement(7451..9430)
                     /gene="aegA"
                     /locus_tag="J1Y83_003306"
     CDS             complement(7451..9430)
                     /gene="aegA"
                     /locus_tag="J1Y83_003306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent uric acid utilization protein
                     AegA"
                     /protein_id="HBA7631423.1"
                     /translation="MNRFIMANSQQCLGCHACEIACVMAHNDEQHVLSQHHFHPRITV
                     IKHQQQRSAVTCHHCEDAPCARSCPNGAISHVDDSIQVNQQKCIGCKSCVVACPFGTM
                     QIVLTPVAVGKVKATAHKCDLCAGRENGPACVENCPADALQLVTDAALSGMAKSRRLR
                     TVRQEQQPWHASTAAQEMPVMSKVEQMQATPARGEPDKLAIEARKTGFDEIYLPFRAD
                     QAQREASRCLKCGEHSVCEWTCPLHNHIPQWIELVKAGNIDAAVELSHQTNTLPEITG
                     RVCPQDRLCEGACTIRDEHGAVTIGNIERYISDQALAKGWRPDLSHVTKVDKRVAIIG
                     AGPAGLACADVLTRNGVAVTVYDRHPEIGGLLTFGIPSFKLDKSLLARRREIFSAMGI
                     HFELNCEVGKDVSLNSLLEQYDAVFVGVGTYRSMKAGLPNEDAPGVYDALPFLIANTK
                     QVMGLEELPEEPFINTAGLNVVVLGGGDTAMDCVRTALRHGASNVTCAYRRDEANMPG
                     SKKEVKNAREEGANFEFNVQPVALELNEQGHVCGIRFLRTRLGEPDAQGRRRPVPVEG
                     SEFVMPADAVIMAFGFNPHGMPWLESHGVTVDKWGRIIADVESQYRYQTTNPKIFAGG
                     DAVRGADLVVTAMAEGRHAAQGIIDWLGVKSVKSH"
     gene            9636..11336
                     /gene="narQ"
                     /locus_tag="J1Y83_003307"
     CDS             9636..11336
                     /gene="narQ"
                     /locus_tag="J1Y83_003307"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite two-component system sensor
                     histidine kinase NarQ"
                     /protein_id="HBA7631424.1"
                     /translation="MIVKRPVSASLARAFFYIVLLSILSTGIALLTLASSLRDAEAIN
                     IAGSLRMQSYRLGYDLQSGSPQLNAHRQLFQQALHSPVLTNLNVWYVPEAVKTRYAHL
                     NANWLEMNNRLSKGDLPWYQANINNYVNQIDLFVLALQHYAERKMLLVVAISLAGGIG
                     IFTLVFFTLRRIRHQVVAPLNQLVTASQRIEHGQFDSPPLDTSLPNELGLLAKTFNQM
                     SSELHKLYRSLEASVEEKTRDLHEAKRRLEVLYQCSQALNTSQIDVHCFRHILQIVRD
                     NEAAEYLELNVGDNWRISEGQPNPELPMQILPVTMQETVYGELHWQNSHVSSSEPLLN
                     SVSSMLGRGLYFNQAQKHFQQLLLMEERATIARELHDSLAQVLSYLRIQLTLLKRSIP
                     EDNATAQSIMADFSQALNDAYRQLRELLTTFRLTLQQADLPSALREMLDTLQNQTSAK
                     LTLDCRLPTLALDAQMQVHLLQIIREAVLNAMKHANASEIAVSCVTAPDGNHTVYIRD
                     NGIGIGEPKEPEGHYGLNIMRERAERLGGTLTFSQPSGGGTLVSISFRSAEGEESQLM
                     "
     gene            11500..14613
                     /gene="acrD"
                     /locus_tag="J1Y83_003308"
     CDS             11500..14613
                     /gene="acrD"
                     /locus_tag="J1Y83_003308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease AcrD"
                     /protein_id="HBA7631425.1"
                     /translation="MANFFIDRPIFAWVLAILLCLTGTLAIFSLPVEQYPDLAPPNVR
                     VTANYPGASAQTLENTVTQVIEQNMTGLDNLMYMSSQSSGTGQASVTLSFKAGTDPDE
                     AVQQVQNQLQSAMRKLPQAVQNQGVTVRKTGDTNILTIAFVSTDGSMDKQDIADYVAS
                     NIQDPLSRVNGVGDIDAYGSQYSMRIWLDPAKLNSFQMTAKDVTDAIESQNAQIAVGQ
                     LGGTPSVDKQALNATINAQSLLQTPEQFRDITLRVNQDGSEVRLGDVATVEMGAEKYD
                     YLSRFNGKPASGLGVKLASGANEMATAELVLNRLDELAQYFPHGLEYKVAYETTSFVK
                     ASIEDVVKTLLEAIALVFLVMYLFLQNFRATLIPTIAVPVVLMGTFSVLYAFGYSVNT
                     LTMFAMVLAIGLLVDDAIVVVENVERIMSEEGLTPREATRKSMGQIQGALVGIAMVLS
                     AVFVPMAFFGGTTGAIYRQFSITIVAAMVLSVLVAMILTPALCATLLKPLKKGEHHGQ
                     KGFFAWFNQMFNRNAERYEKGVAKILHRSLRWIVIYVLLLGGMVFLFLRLPTSFLPLE
                     DRGMFTTSVQLPSGSTQQQTLKVVEQIEKYYFTHEKDNIMSVFATVGSGPGGNGQNVA
                     RMFIRLKDWSERDSKTGTSFAIIERATKAFNKIKEARVIASSPPAISGLGSSAGFDME
                     LQDHAGAGHDALMAARNQLLALAAENPELTRVRHNGLDDSPQLQIDIDQRKAQALGVA
                     IDDINDTLQTAWGSSYVNDFMDRGRVKKVYVQAAAPYRMLPDDINLWYVRNKDGGMVP
                     FSAFATSRWETGSPRLERYNGYSAVEIVGEAAPGVSTGTAMDIMESLVKQLPNGFGLE
                     WTAMSYQERLSGAQAPALYAISLLVVFLCLAALYESWSVPFSVMLVVPLGVIGALLAT
                     WMRGLENDVYFQVGLLTVIGLSAKNAILIVEFANEMNQKGHDLFEATLHACRQRLRPI
                     LMTSLAFIFGVLPMATSTGAGSGGQHAVGTGVMGGMISATILAIYFVPLFFVLVRRRF
                     PLKPRPE"
     gene            complement(14712..14771)
                     /gene="ypfM"
                     /locus_tag="J1Y83_003309"
     CDS             complement(14712..14771)
                     /gene="ypfM"
                     /locus_tag="J1Y83_003309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YpfM"
                     /protein_id="HBA7631426.1"
                     /translation="MIERELGNWKDFIEVMLRK"
BASE COUNT         3512 a         3982 c         3909 g         3587 t
ORIGIN      
        1 gcgtgagagt taaaaaagaa ttacagcggt tgggtttgcg cttcaaccac ggccagcgcc
       61 accatgttga cgatacgacg taccgatgcg atcggcgtta acacgtgaac cggtttcgcc
      121 acccccatca gcaccgggcc gacagtcaca ccttccgagc tggaaacacg cagtaagttg
      181 taactaatgc gggcagcttc catgttcggc atcaccagaa tattagcgga acctttcaaa
      241 gggctgtccg gcatacggtc gttgcgaatc gcttccacca gcgctgcatc gccgtgcatt
      301 tcaccatcaa tcatcagttc tggcgcacgt tccctgacca gttccagcgc ctgacgcatt
      361 ttgctcgacg acgggcagtc agaagaacca aagttggagt gcgacaacag agccacacgc
      421 ggctcaatac caaaacgacg aacagtttct gccgccatca aggtgatctc cgccagctct
      481 tctgcatcag gctcatcatt gacgtaggta tcggcaataa aggtgttacc actcggcaac
      541 agcagcgcgt tcattgcacc tgcggtgtga acgccatcgc gataaccaaa gacatttttc
      601 accacgctaa agtgttcatg ataatcgccc accgtaccgc aaatcattgc atcggcttcg
      661 ccacgctgaa ccatgatcgc gccgatcact gtcgggttac tgatcagcgc acgctgcgcc
      721 tgctcctgag tgacaccgcg acgcttcatg atctggaagt attcggtcca gtactcttta
      781 aagcgcggat cggattcgtt attgacgatc tcaaaatcaa cgcccgcctt gatctgcaag
      841 cccagtttct gaatgcgcat ttcgatcacg ttcggacgac caataaggat cggtttcgcc
      901 agtcccagcg ttaccagttc ctgagtggca tgcagaacgc gcgcctcttc cccttccggc
      961 agaactacgc gcttcggcgc tttgcgagcc tgggagaaaa tcggcttcat aaacaggtta
     1021 gttttgtaaa cgaactccgt cagcttgtcg atgtagacgt cgaaatcagc aatcggacgc
     1081 gtcgccacgc ccgactccat cgccgctttc gccaccgcag gagcgatctt aacgatcaag
     1141 cgcggatcaa acggttttgg aatgatgtat tccggaccaa agctcagatc ctgatcgcca
     1201 tacgctgaag ccaccacttc actctgttcc gcatgggcca gttctgcaat cgcgcgtacc
     1261 gccgccagtt tcatctcttc gttgatggcg gttgcgccaa cgtccagcgc gccacggaag
     1321 ataaacggga agcaaaggac gttgttcacc tggttcgggt agtcggaacg gccggtacag
     1381 atgatggcat ccggacgcac ttctttcgcc agcggcggca gaatttccgg ttccgggttc
     1441 gccagcgcca ggatcattgg cgcacgggcc attttcttca ccatttcctg ggtcagcact
     1501 ttcgggccgg aacagccgag gaaaatatcc gcgccttcaa tcacatcatc gagggtacgt
     1561 ttaccgtcat ccaccaccgc gtatgcggct ttggtttccg ccatgtttgg ctcacggccc
     1621 tgatagataa cgccttttga atcgcaaacc acgatgttat gtttttgcag acccagcgct
     1681 accagcaggt tcatacaggc gattgctgcg gcacccgcgc cagaaaccac catccgcacg
     1741 tcggagatgt ttttctccac cacgcgcaag ccgttgagga tggcggcagt gctgataatt
     1801 gccgtgccgt gctgatcgtc gtggaatacc ggaatattca tccgctcgcg cagtttctgt
     1861 tcaatataga aacattctgg cgctttaatg tcttcgaggt tgatgccgcc aaaggtcggt
     1921 tcgagcgcag cgacgacttc aataaatttg tccgggtcga gttcatcaac ttctatgtca
     1981 aatacatcaa tcccggcgaa tttcttaaac agaacgccct tgccttccat caccggtttg
     2041 cctgccagcg cgccaatgtt gcctaacccc agcaccgccg taccgttaga gatcaccgct
     2101 accaggttac cgcgcgcggt atatttgtag gcttttaacg ggtctttttc gatttcaaga
     2161 caaggtgcgg caacgcctgg tgagtaggcc agcgccagat cgcgctgcgt tgccagaggc
     2221 ttggttggag aaacctggat tttccctgga actggaaatt catggaaatc aagtgcactt
     2281 tgttttaact ggtcatccat ttggttgttc ctttcacgta acgttcacaa ataaagtgtg
     2341 tgggcaacag cccctgtcca caacgtggcg cacattatta ccctgccgga gtctacagac
     2401 tttgagcaag tccaaactct caccattaat ataatgtttt ggtaataatc ctataacact
     2461 gatgttacct gcttaatcca gcaataccat gcctgtctgc tatgcttttt tgatgcgttt
     2521 agcgaaattt ctcagaagtg tgaattaacg cactcatcta acactttact tttcaaggag
     2581 tatttcctat gaacgagtta gacggcatca aacagttcac cactgtcgtg gcagacagcg
     2641 gcgatattga gtccattcgc cattatcatc cccaggatgc caccaccaat ccttcgctgt
     2701 tactcaaggc tgccggatta tcacaatatg agcatttaat agacgatgct atcgcctggg
     2761 gtaaaaaaaa tggcaagacc caggaacaac aggtggtcgc agcgtgtgac aaactggcgg
     2821 tcaatttcgg tgctgaaatt ctcaaaatcg tacccggtcg cgtgtcaaca gaagttgatg
     2881 cacgcctctc ttttgataaa gaaaagagta ttgagaaggc gcgccatctg gtggacttgt
     2941 atcagcaaca aggcgttgag aaatcgcgca ttctgatcaa gctggcttcg acctgggaag
     3001 gaattcgcgc ggcagaagag ctggaaaaag aaggtatcaa ctgcaacctg acgctgctgt
     3061 tttcttttgc acaggcgcgg gcctgtgcag aagcaggcgt ttttctgatt tcgccgtttg
     3121 tcgggcgtat ttatgactgg tatcaggcac gcaagccgat ggacccgtat gtggtggaag
     3181 aagatccagg cgttaaatca gtacgcaata tctacgacta ctataagcaa caccactatg
     3241 aaaccattgt gatgggcgcg agcttccgtc gcaccgaaca aatcctcgcc ttaaccggct
     3301 gcgaccgact gactatcgca ccgaatttgc tgaaggagct acaggaaaaa gtttcgccag
     3361 tggtacgtaa attaatccca ccttctcaga cgttcccacg cccagctccc atgagcgaag
     3421 cggaattccg ttgggagcac aatcaggatg cgatggcggt agaaaaactg tctgaaggca
     3481 ttcgtctgtt cgccgttgat caacgcaaac tggaagatct tcttgccgcc aaactataaa
     3541 ccagccacgg agtgttatat gtcccgaaaa gaccttgcca atgcgattcg cgcactcagt
     3601 atggatgcgg tacaaaaagc caattctggt catcccggcg cgccgatggg catggctgat
     3661 attgccgaag tgctgtggaa cgattttctt aaacataacc ccaccgaccc aacctggtat
     3721 gaccgtgacc gctttattct ttccaacggt cacgcgtcga tgctgctcta cagtttgctg
     3781 catctgaccg gttacgacct gccactggaa gaactgaaga acttccgtca gttgcattcg
     3841 aaaacccctg gtcacccgga aatcggctat acgccaggtg ttgaaaccac caccggtcct
     3901 cttggacaag gtttggcgaa cgccgtcggg ctggcgatag cagagcgtac gctggcggcg
     3961 cagtttaatc agccggatca tgaaattgtc gatcacttca cctatgtgtt tatgggcgac
     4021 ggctgcctga tggaaggtat ctctcacgaa gtctgttcgc tggcgggcac gctggggctg
     4081 ggcaagctga ttggttttta cgatcacaac ggtatttcca tcgacggtga aaccgaaggc
     4141 tggtttaccg acgatacggc aaaacgtttt gaagcctatc actggcatgt ggtccacgag
     4201 atcgacggcc acgatccgca ggcggtgaag gaagcgatcc ttgaagcgca aagcgtgaaa
     4261 gataagccgt cgctgattat ctgccgtacg gtgattggct ttggttcgcc gaataaagca
     4321 ggtaaggaag aggcgcacgg cgcaccgctg ggggaagaag aagtagctct ggcacggcaa
     4381 aaactgggct ggcaccatcc gccatttgag atccctaaag agatttatca cgcctgggat
     4441 gcccgtgaaa aaggcgaaaa agcgcagcag agctggaatg agaagtttgc cgcctataaa
     4501 aaggctcatc cgcaactggc agaagagttt acccgtcgaa tgagcggcgg tttgccgaag
     4561 gactgggaga aaacgactca gaaatatatc aatgagttgc aggcgaatcc ggcgaaaatc
     4621 gctacccgta aggcttcgca aaatacgctt aacgcttacg ggccgatgct gcctgagttg
     4681 ctcggcggtt cggcggatct ggctcccagc aacctgacca tctggaaagg ttctgtttcg
     4741 ctgaaggaag atccggcggg caactacatt cactacgggg tgcgtgaatt tggtatgacc
     4801 gctatcgcca acggcatcgc gcaccacggc ggctttgtgc cgtataccgc gacgttcctg
     4861 atgtttgttg aatacgcccg taacgccgcg cggatggcgg cactgatgaa agcgcggcag
     4921 attatggttt atacccacga ctcaattggt ctgggcgaag atggtccgac gcaccaggct
     4981 gttgagcaac tggctagtct gcgcttaacg ccaaatttca gcacctggcg accgtgcgat
     5041 caggtggaag cggcggttgg ctggaagctg gcggttgagc gccacaacgg accgacagca
     5101 ctgatcctct caaggcagaa tctggcacag gtagaacgaa caccggatca ggttaaagag
     5161 attgctcgtg gcggttatgt gctgaaagac agcggcggta agccagatat tattctgatt
     5221 gccaccggtt cagagatgga aatcaccctg caagcggcag agaaattagc gggagaaggt
     5281 cgcaatgttc gcgtggtttc cctgccctcg accgatattt tcgatgccca ggatgaggaa
     5341 tatcgcgagt cggtactgcc ttcaaatgtt tctgcccgcg tggcagtaga agcgggtatt
     5401 gccgattact ggtacaaata tgttggtctg aaaggggcaa ttgtcgggat gacaggttat
     5461 ggggaatctg ctccggcgga taagctgttc ccgttctttg gctttaccgc cgagaatatt
     5521 gtggcaaaag cgcataaggt actgggagtg aaaggtgcct gatggtgatc gccggatgct
     5581 gattgtcgga tgtgacgctg gtgcgtctta tccggtctac atgtccccgc cattttgttt
     5641 aacgggtgat ccacaacgtg ggccaggcgt ctggcccatg ccagttatcg caggtgggtt
     5701 ctgcggcgta acgcaccagg cgaaaacgct gaccgtcaaa gcgccagcgc gcctgaatgc
     5761 cacaatcgct taatccgcgc ccttttgcta aggtcaccag ttcacgcgat ttttcatcga
     5821 atgttgcgtt catcagttcc agttcattgg tctcctgacc gttgttgaac ggcaaacgca
     5881 accgtaccgg gcgcgaggcc agcggctttt tacgcgacac aatccatgcc aaatcaatgg
     5941 tgttataggc ccctgcctca cagctaatca tcatcagcgc tttatcatcg gtcagcgcag
     6001 tgacattcac ctcacgacgt aacggatcaa gcgaacagcg cagaccattc atccgccagt
     6061 ttccataatc cagcaaatcg ttacgttctt cgcgtgagag tggcgtcggc gttgggttaa
     6121 ccaccgcgac ctctttcagc gcaggcgctg gcggtacgct gagcggcggt tcgtcccctt
     6181 tctttatcca cgcggtctca ctgccaacgc gcttttgctg ggcatcaata aacaacaacg
     6241 ccgcttttaa gccactcaga gaaatagtct gattgccgtc gcgtaaggtg atcgccctcc
     6301 cttcctgaat catctgcaaa aacgcggtga tggttgccgt atcatcggtt actaataacc
     6361 atggtgaaat ccgccacttg tcaccactta acgccaacgg ctcaccatct aacagcagcc
     6421 gtggcgctat ctccccttct gacgcatccg gcgacttcaa cccgccgcgc tcaatacgta
     6481 aaacggcatc ggtatgcgcc ccggcgctgc ggctcagggt catcaccagt ccattatgat
     6541 cgcccgtatt gcgcgctaca cagaaatttt gattattaca ggtgacctgc cagtcggaaa
     6601 acgcccgttg cgctggtgcc gcccacacca aagacatcgg caacaaaacg aaaaaaaaga
     6661 gaaggaaaat gcgatatcgc atggacggta tgaccccaga agttcaaaca aacagcacaa
     6721 gtcgtatctt cgttgcgcag gtagcgcggc tcaatcggat ttgtcggatt gagccaataa
     6781 taaacaacga aatattattg ttatttttca gtccattaaa tgtgacattt gcaaatagtt
     6841 aagcaataac accgtcttac cgtcacgtat ctcgccggtt ttaatcatct ccagcgcctg
     6901 gctgaacggc agctcaagca cttcaatatc ttcatcttcg acaccaccac cggcgttagc
     6961 gcgctggctg tcactgtatt cggcgataaa aaagtggatt agctcagtca caccgcctgg
     7021 cgacatatac agttcaaata atttgcggac ttcgccaact tcatagcccg tctcttcaat
     7081 cgcttccttg cgaatgcaca cttccggttc gtcgttatcc agcagcccgg cgcaggtttc
     7141 aatcagttgc ccgctttcat tgccattaac ccaggtagcg acgcggaact ggcgaatcag
     7201 aaccacggtc tttttcttcg cgttgtacag gaggatcgtc gcaccattac cgcgatcgta
     7261 aacttcacgt ttatggcgga taacttcgcc atctttgcgc gtgagatcgt aagtaatgtt
     7321 gtgcagggtg aaatagttat cggagagaat tttgtcttta acgagggtga tttgttgcgt
     7381 cataccgact ccacagcgcg aaatgaacaa ttatcttacg ctgtgaagtc gggtttgtct
     7441 gcgcaggcta tcagtgagat ttgactgatt ttacgcccag ccagtcaatg atcccctgtg
     7501 ccgcatgacg tccttctgcc attgcggtaa ccaccagatc cgcaccacgc acggcgtcac
     7561 caccggcgaa gattttcgga ttcgtggtct ggtaacggta ctggctttcc acatccgcga
     7621 tgatgcggcc ccatttgtct accgttacac cgtgcgactc cagccacggc atcccgtgcg
     7681 gattgaagcc aaacgccata atcaccgcat cggctggcat gacaaattca ctgccttcca
     7741 ccggcactgg acgccgacgt ccctgggcgt ccggctcgcc aagacgcgtg cgcaggaaac
     7801 gaatcccgca gacgtgacct tgttcattca gttcaagcgc caccggctgg acgttaaatt
     7861 cgaagttggc cccctcttca cgggcgttct tcacctcttt cttcgagcct ggcatgttag
     7921 cttcatcacg acgataagcg caggtgacgt tactcgcgcc gtggcgcagc gcagtacgca
     7981 cacagtccat cgcggtgtcg ccgccgccca gtaccacgac gttaagtccg gcagtattga
     8041 taaacggctc ttccggtagc tcttcgagtc ccatcacctg tttagtgttg gcgatgagga
     8101 acggcagcgc gtcataaacg cccggcgcat cttcattggg taaacccgct ttcatggaac
     8161 ggtaagtgcc tacgccaacg aagaccgcgt cgtattgttc caaaagcgaa ttcaaagaaa
     8221 catctttacc cacttcacaa ttgagttcga agtgaatccc catcgcgctg aagatttccc
     8281 gacggcgtgc cagcagggat ttatccagtt tgaaagaagg aatgccgaaa gtgagcaagc
     8341 caccgatttc tggatggcga tcgtacaccg tcaccgccac gccattgcgg gtgagaacat
     8401 ctgcacaggc cagccctgcc ggacccgcac cgataatcgc cacccgcttg tccactttgg
     8461 tgacatggct taagtcagga cgccaacctt tcgccaacgc ctgatctgaa atgtagcgtt
     8521 caatgttacc gatagttact gcgccgtgct catcgcgaat agtacaggca ccttcacaca
     8581 aacggtcttg cgggcaaacg cgtccggtaa tttccggcag ggtgttggtc tggtgagaaa
     8641 gctcgacggc ggcgtcgatg tttccggctt tcaccagttc aatccactgc ggtatatggt
     8701 tatgcagcgg gcaggtccat tcacaaacgc tgtgctcgcc gcacttaagg cagcgcgagg
     8761 cttcccgttg tgcctgatcg gcacggaatg gcagataaat ttcatcaaaa ccggttttgc
     8821 gcgcttcaat cgccagttta tctggctcgc cacgcgcggg cgttgcctgc atttgctcga
     8881 ctttactcat taccggcatt tcttgcgccg cggtactggc atgccacggt tgttgttcct
     8941 gacgtacggt gcgcaagcgg cgggatttcg ccatgccgga gagtgcggcg tcagtgacca
     9001 gttgcagcgc gtccgccggg caattttcga cacaggcagg accgttttcg cgccctgcac
     9061 aaaggtcgca tttatgcgcc gtggctttta cttttcctac cgcaacgggt gtcagaacga
     9121 tttgcatcgt accaaatgga caggccacca cgcaggattt acagccaata cacttttgct
     9181 gattgacctg aatgctgtca tcaacgtggc tgattgcgcc attagggcag ctacgggcgc
     9241 agggcgcatc ttcacaatgg tgacaggtca ctgcactacg ttgctgctga tgtttgataa
     9301 ccgtaattcg gggatgaaaa tggtgttggc tcaggacatg ttgctcatca ttgtgagcca
     9361 tgacgcaggc gatttcacaa gcatgacaac ccagacattg ctgactgttg gccataataa
     9421 aacgattcat aacgaccttc ttttttggtt gtaaaaacct tattctttat atgagtgttg
     9481 ttattacccg acttacaggg gaatcggcaa tgttcatgtg cccagaataa gtaactattt
     9541 cgctataaac tgtggcagat caaataatcc catcactgac taaatttcgt ttcagcgaac
     9601 tggaacatta atgatttttt gtggagaaga cgcgtgtgat tgttaaacga cccgtctcgg
     9661 ccagtctggc ccgggccttt ttttacattg tgctgctgtc gattctttcc acgggtatcg
     9721 ctctgctaac gctggcgagc agtttacgcg acgctgaggc tatcaatatt gccggatcgc
     9781 tgcgtatgca gagttaccgc ctgggctacg acttgcaaag tggcagtcca caactcaatg
     9841 cacatcgcca gttatttcag caggcactgc attcaccggt attaaccaat ctcaacgtct
     9901 ggtatgtgcc agaggcagta aaaacccgct atgcgcatct gaatgccaac tggctggaga
     9961 tgaataatcg gctcagcaag ggcgacttgc cgtggtatca ggccaatatt aataattatg
    10021 ttaatcagat agacctgttc gtgctggctt tacagcacta cgccgaacgc aaaatgctgc
    10081 tggtggtggc gatctccctg gctggcggca tcggtatttt cacgctggtc ttttttactt
    10141 tgcgccgcat acgccatcag gtggttgccc cgctgaatca gctggttacc gccagtcagc
    10201 gtattgaaca cgggcagttc gactcgccgc cgctggatac cagcctgccg aatgagcttg
    10261 gtctgcttgc aaaaaccttt aaccagatgt cgagcgagct gcataaattg taccgttcgc
    10321 tggaggcgtc agtagaagaa aagacccgcg atctccacga ggccaagcgt cgtctggagg
    10381 tgttgtatca gtgttcgcag gcgctaaaca ccagccagat tgatgtgcat tgtttccgcc
    10441 atattttgca gattgttcgc gacaatgaag cggctgaata tctggagtta aatgtcggtg
    10501 acaactggcg gattagcgaa gggcagccaa acccggaatt gccgatgcag attttaccgg
    10561 tgacaatgca agagacggtt tacggcgaac tgcactggca aaatagtcac gtttcatcat
    10621 cagaaccgct gcttaacagc gtttcgtcga tgctgggacg cggtttgtac tttaatcagg
    10681 cgcagaagca ttttcagcag ttattgttga tggaagaacg tgcgaccatc gcccgcgaat
    10741 tgcacgactc gctggctcag gtactttctt acttacgtat ccagttgacg ttactgaagc
    10801 gttcgatacc ggaagataac gccaccgcac aaagtatcat ggccgatttt tcccaggcgt
    10861 tgaatgatgc ttatcggcag ttacgcgagc tgttaactac tttccgcctg acgctgcagc
    10921 aggcggatct cccctccgcg ttgagggaaa tgctggatac gttacaaaat caaaccagcg
    10981 ccaaactgac cctcgactgc cgtctgccaa ccctggcgct ggatgcgcaa atgcaggtgc
    11041 atttgttgca aattattcgc gaagcggtgc tgaatgcgat gaagcacgcc aacgccagcg
    11101 aaatcgccgt tagctgcgtc accgcgccgg atggcaatca cacagtctat attcgcgaca
    11161 acgggattgg catcggtgaa ccgaaagaac ccgaaggcca ttatggtctg aatatcatgc
    11221 gcgaacgcgc ggaaagattg ggtgggacgc tgactttttc acaaccttcc ggcggcggca
    11281 cgttagtaag tattagcttt cgctctgcgg agggtgagga aagtcagctg atgtaatgcc
    11341 tcttattgac caaagaatag tggcacttaa ggttcagtat aaaagggcat gataatttac
    11401 attaactcct ttttttctcc acgattggct cgtaccttgc cgctacagtg aagcaagtca
    11461 agcctacaac gatacgcaga aacacgaggt cctcttttaa tggcgaattt ctttattgat
    11521 cgccccattt ttgcctgggt gctggcaatc ctgttgtgtc tgacaggtac cctggcgatt
    11581 ttttcattgc ccgttgaaca ataccccgat ctcgcgccac cgaatgtgcg agtgaccgct
    11641 aactatcccg gcgcatcggc ccagacgctg gaaaataccg tgacccaggt tatcgagcaa
    11701 aatatgaccg gcctcgataa tctgatgtat atgtcatctc agagcagcgg caccggtcag
    11761 gcatctgtca cgttaagttt taaagcaggc accgatccgg acgaagccgt gcagcaagta
    11821 caaaaccagc tacaatcagc catgcgaaag ttaccgcagg cggtgcaaaa tcagggcgtg
    11881 acggtgcgta aaaccggcga taccaacatt ctgaccattg ccttcgtctc taccgatggt
    11941 tcgatggata aacaggatat tgccgattat gttgccagta atattcagga cccgttaagc
    12001 cgcgtgaatg gcgtcgggga tatcgatgcc tatggttcgc aatattccat gcgtatctgg
    12061 ctggacccgg cgaaactcaa cagtttccag atgacggcta aagatgtcac cgatgccatt
    12121 gaatcacaga acgcgcagat tgcggttggg caacttggtg gtacgccttc cgtcgataag
    12181 caggcgctca acgccaccat taacgcccag tcactgctgc aaacaccaga acagttccgc
    12241 gatatcacct tgcgggtgaa tcaggacggc tcagaggtaa ggctgggcga tgtcgccacc
    12301 gtcgaaatgg gggcggagaa atacgattat cttagccgct tcaatggtaa gccagcctcc
    12361 gggctggggg taaaactggc ctccggcgcg aacgaaatgg cgacagcgga gctggtgctc
    12421 aatcgtctcg acgagctggc gcagtatttt ccacacggac tggaatacaa ggtggcgtat
    12481 gaaaccacct cgtttgttaa agcctccatt gaagacgtgg tgaaaacgct tctggaagct
    12541 atcgccctgg ttttcctcgt tatgtatctg ttcctgcaaa acttccgcgc cacgctgata
    12601 cccactatcg ccgtgccagt ggtgttgatg ggaaccttct ccgtgcttta cgccttcggt
    12661 tacagtgtca acaccttaac catgttcgcg atggtgctgg cgatcggtct gctggtggat
    12721 gatgccatcg tggtggtgga aaacgtcgaa cgtattatga gtgaggaagg actcactcct
    12781 cgcgaagcca cacgtaaatc gatggggcag atccaggggg cactggtcgg gattgcgatg
    12841 gtgctttcgg cggtatttgt gccaatggcc ttcttcggcg gcaccaccgg tgccatctat
    12901 cgccagttct ctattaccat tgttgcggcg atggtgctgt cagtactggt agcgatgatc
    12961 ctcactcctg ctctgtgtgc cacgctactt aagccactga aaaaaggtga gcatcacggg
    13021 caaaaaggct tttttgcctg gtttaaccag atgtttaacc gcaacgccga acgctacgaa
    13081 aaaggggtgg cgaaaattct ccaccgtagc ctgcgctgga ttgtgattta tgtcctgctg
    13141 cttggcggca tggtgttcct gttcctgcgt ttgccgacgt cgttcttacc gctggaagac
    13201 cgtggcatgt ttactacctc ggtacagttg cccagcggtt caacccagca acagaccctg
    13261 aaagtcgttg agcaaatcga gaaatattac ttcacccatg aaaaagacaa catcatgtcg
    13321 gtgtttgcca ccgtgggttc tggccctggg ggtaacgggc aaaacgtggc gcgaatgttt
    13381 atccgcctga aagactggag cgaacgcgac agtaagaccg gcacctcgtt tgccattatc
    13441 gagcgtgcaa cgaaggcgtt taacaagatt aaagaagctc gcgttatcgc cagcagcccg
    13501 ccagcaatta gcggtcttgg tagttctgcg ggttttgata tggagttgca ggaccacgct
    13561 ggagcgggtc acgatgcgct gatggcagca cgaaaccagt tgctggcgct ggcagcggaa
    13621 aacccggagc taacccgtgt gcgccataac ggcctcgacg acagtccgca gttgcagatt
    13681 gatatcgacc agcgtaaagc tcaggcgctg ggcgttgcta tcgacgatat taacgacaca
    13741 ctgcaaaccg cctggggttc gagctatgtg aatgacttta tggatcgcgg tcgcgtgaag
    13801 aaagtctatg tgcaggcagc tgcgccgtat cgcatgctgc cagatgacat caatctctgg
    13861 tatgtccgaa ataaagatgg cggcatggtg cccttctctg ctttcgcgac ctcacgctgg
    13921 gagacaggct cgccgcgtct ggaacgctat aacggttatt ctgcggttga gattgttggg
    13981 gaagccgcac cgggggtcag taccggtacg gcgatggata ttatggaatc gttagtgaag
    14041 cagctgccaa acggctttgg tctggagtgg acggcgatgt cgtatcagga gcggctttcc
    14101 ggcgcgcagg ctccggcgct gtacgccatt tccttgctgg tggtattcct gtgtctggcg
    14161 gcattgtatg aaagctggtc ggtgccgttc tcggtaatgc tggtcgtgcc gctgggggtt
    14221 atcggcgcgc tgctggcaac ctggatgcgc gggctggaaa acgatgttta cttccaggtg
    14281 ggcctgttaa cggtcattgg tttatcggcg aaaaacgcca tcctgatcgt cgaatttgct
    14341 aacgagatga accaaaaagg ccacgacctg tttgaagcga cgctccacgc ctgccgtcag
    14401 cgtttacgcc cgattctgat gacctcgctg gcatttatct tcggcgtatt gccaatggca
    14461 accagcacag gtgccggttc cggtggtcag catgcggtgg gtactggcgt aatgggcggg
    14521 atgatttcgg ccactattct ggctatttac ttcgtgccgc tgttctttgt gctggtgcgc
    14581 cgccgcttcc cgctgaagcc gcgcccggaa taagcaataa aaaaggcgac atgccaatgt
    14641 gtcgcccttt tcaactttcc gattaagaac ctgctcagcg ggttcttgct gtttgtactt
    14701 tgtctcagga attacttacg aagcataact tcgataaagt ctttccagtt ccccagttca
    14761 cgttcaatca taacaacctc tcttataatt atgggtattc tacggaaaca atataccgtg
    14821 gtgaagctaa tttactcgat tgctgcgatg actacctccg ggggacaaac cttatgtaaa
    14881 tactatggtc ctgcggtgat gatttgtatg tgatacaccg caacatttcg agatattcat
    14941 acgacatcta atactgattt aattctggtt aaaatacaga cagataacaa
//
LOCUS       DADSYM010000123        14421 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2415
            SAMN08039579-rid11723173.denovo.124, whole genome shotgun sequence.
ACCESSION   DADSYM010000123 DADSYM010000000
VERSION     DADSYM010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039579
            Sequence Read Archive: SRR6362533
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14421)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14421)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980162.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 03:39:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,484
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 4,347
            CDSs (with protein)               :: 4,347
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 43 of 85
            Pseudo Genes (incomplete)         :: 37 of 85
            Pseudo Genes (internal stop)      :: 19 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14421
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039579-rid11723173.denovo.124"
                     /strain="LGE2415"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8302 N 95.9628 W"
                     /collection_date="2015-08-24"
     gene            complement(248..1897)
                     /gene="pgi"
                     /locus_tag="J1Y83_003310"
     CDS             complement(248..1897)
                     /gene="pgi"
                     /locus_tag="J1Y83_003310"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HBA7631427.1"
                     /translation="MKNINPTQTAAWQALQKHFDEMKDVTIADLFAKDGDRFSKFSAT
                     FDDQMLVDYSKNRITEETLAKLQDLAKECDLAGAIKSMFSGEKINRTENRAVLHVALR
                     NRSNTPILVDGKDVMPEVNAVLEKMKTFSEAIISGEWKGYTGKAITDVVNIGIGGSDL
                     GPYMVTEALRPYKNHLNMHFVSNVDGTHIAEVLKKVNPETTLFLVASKTFTTQETMTN
                     AHSARDWFLKAAGDEKHVAKHFAALSTNAKAVGEFGIDTANMFEFWDWVGGRYSLWSA
                     IGLSIVLSIGFDNFVELLSGAHAMDKHFSTTPAEKNLPVLLALIGIWYNNFFGAETEA
                     ILPYDQYMHRFAAYFQQGNMESNGKYVDRNGKVVDYQTGPIIWGEPGTNGQHAFYQLI
                     HQGTKMVPCDFIAPAITHNPLSDHHQKLLSNFFAQTEALAFGKSREVVEQEYRDQGKD
                     PATLDYVVPFKVFEGNRPTNSILLREITPFSLGALIALYEHKIFTQGVILNIFTFDQW
                     GVELGKQLANRILPELKDDKEISSHDSSTNGLINRYKAWRG"
     gene            2422..3771
                     /gene="lysC"
                     /locus_tag="J1Y83_003311"
     CDS             2422..3771
                     /gene="lysC"
                     /locus_tag="J1Y83_003311"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine-sensitive aspartokinase 3"
                     /protein_id="HBA7631428.1"
                     /translation="MSEIVVSKFGGTSVADFDAMNRSADIVLSDANVRLVVLSASAGI
                     TNLLVALAEGLEPGERFEKLDAIRNIQFAILERLRYPNVIREEIERLLENITVLAEAA
                     ALATSPALTDELVSHGELMSTLLFVEILRERDIQAQWFDVRKVMRTNDRFGRAEPDVA
                     ALAELAALQLLPRLNEGLVITQGFIGSENKGRTTTLGRGGSDYTAALLAEALHASRVD
                     IWTDVPGIYTTDPRVVSAAKRIDEIAFAEAAEMATFGAKVLHPATLLPAVRSDIPVFV
                     GSSKDPRAGGTLVCNKTENPPLFRALALRRNQTLLTLHSLNMLHSRGFLAEVFGILAR
                     HNISVDLITTSEVSVALTLDTTGSTSTGDTLLTQSLLMELSALCRVEVEEGLALVALI
                     GNDLSKACGVGKEVFGVLEPFNIRMICYGASSHNLCFLVPGEDAEQVVQKLHFNLFE"
     gene            4024..4296
                     /locus_tag="J1Y83_003312"
     CDS             4024..4296
                     /locus_tag="J1Y83_003312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3811 domain-containing protein"
                     /protein_id="HBA7631429.1"
                     /translation="MALPRITQKEMTEREQRELKTLLDRARIAHGRVLTNSETNSIKK
                     EYIDKLMVEREAEAKKARQLKKKQAYKPDPEASFSWSANTSTRGRR"
     gene            complement(4297..5169)
                     /gene="rluF"
                     /locus_tag="J1Y83_003313"
     CDS             complement(4297..5169)
                     /gene="rluF"
                     /locus_tag="J1Y83_003313"
                     /EC_number="5.4.99.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2604) synthase RluF"
                     /protein_id="HBA7631430.1"
                     /translation="MLPDSSVRLNKYISESGICSRREADRYIEQGNVFLNGKRATIGD
                     QVKSGDVVKVNGQLIEPRESEDLVLIALNKPVGIVSTTEDGERDNIVDFVNHSKRVFP
                     IGRLDKDSQGLIFLTNHGDLVNKILRAGNDHEKEYLVTVDKPITDEFIRGMGAGVPIL
                     GTVTKKCKVKKEAPFVFRITLVQGLNRQIRRMCEHFGYEVKKLERTRIMNVSLSGIPL
                     GEWRDLTDDELIDLFKLIENSSSEAKPKAKAKPKTVGIKRPVVKMEKTAEKGGRPASN
                     GKRFTSPGRKKKGR"
     gene            5517..6464
                     /locus_tag="J1Y83_003314"
     CDS             5517..6464
                     /locus_tag="J1Y83_003314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding transcriptional regulator"
                     /protein_id="HBA7631431.1"
                     /translation="MENSDDIRLIVKIAQLYYEQDMTQAQIARELGIYRTTISRLLKR
                     GRDQGIVTIAINYDYNENLWLEQQVKQKFGLKDVVVVSGNDEDEDTQLAMMGLHGAQL
                     LDRLLEPGDIVGFSWGRAVSALVENLPQAGQSRQLICVPIIGGPSGKLESRYHVNTLT
                     YSAAAKLKGESHLADFPALLDNPLIRNGIMQSQHFKTISAYWDNLDVALVGIGSPAIR
                     DGANWHAFYGGEESDDLNARQVAGDICSRFFDIHCAMVETNMSEKTLSIEMNKLKQAR
                     YSIGIAMSEEKYSGIVGALRGKYINCLVTNSSTAELLLK"
     gene            6534..7340
                     /locus_tag="J1Y83_003315"
     CDS             6534..7340
                     /locus_tag="J1Y83_003315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HBA7631432.1"
                     /translation="MQTWLNLQDKIIIVTGGASGIGLAIVDELLAQGANVQMVDIHCG
                     DGQYESHKGYQFWPTDISSAKEINHTVAEIIQRFGRIDGLVNNAGVNFPRLLVDEKAP
                     AGQYELNEAAFEKMVNINQKGVFLMSQAVARQMVKQHDGVIVNVSSESGLEGSEGQSC
                     YAATKAALNSFTRSWSKELGKHGIRVVGIAPGILEKTGLRTPEYEEALAWTRNITVEQ
                     LREGYTKNAIPIGRAGRLAEVADFVCYLLSERASYITGVTTNIAGGKTRG"
     gene            7350..7757
                     /locus_tag="J1Y83_003316"
     CDS             7350..7757
                     /locus_tag="J1Y83_003316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sorbose IIA component"
                     /protein_id="HBA7631433.1"
                     /translation="MVNAIFCAHGKLACAMLESVQMVYGDARVEAVEFVPGENAGDIV
                     AKLEKLVSIHNQDEWLIAVDLQCGSPWNAAAMLAMRNPRLRVISGLSLPLALELVDNQ
                     DSMNIDELCEHLTQIAKQTCVVWRQVATAEEDF"
     gene            7757..8251
                     /locus_tag="J1Y83_003317"
     CDS             7757..8251
                     /locus_tag="J1Y83_003317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
                     /protein_id="HBA7631434.1"
                     /translation="MNITLARIDDRLIHGQVTTVWSKVANAQRIIICNDEVYNDEVRR
                     TLLRQAAPPGMKVNVVNIEKAVAVYHNPQYQDETVFYLFTRPQDALAMVRQGVKIGTL
                     NIGGMAWRPGKKQLTKAVSLDDDDINAFHELNNLGIILDLRVVASDPSINIIDKINEQ
                     LIAN"
     gene            8317..9114
                     /locus_tag="J1Y83_003318"
     CDS             8317..9114
                     /locus_tag="J1Y83_003318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HBA7631435.1"
                     /translation="MEISTLQIIAIFLFSCIAGMGSVLDEFQTHRPLIACTVIGLILG
                     DLKTGIMLGGTLELIALGWMNVGAAQSPDSALASIISAILVIVGQQSIATGIAIALPV
                     AAAGQVLTVFARTITVVFQHAADKAAEEARFRTLDILHVSALGVQALRVAIPALIVSL
                     FVSADMVSNMLSAIPEFVTRGLQIAGGFIVVVGYAMVLRMMGVKYLMPFFFLGFLAGG
                     YLDLSLLAFGGVGVIMALLYIQLNPQWRKAEPHPQTTTITALDQLDD"
     gene            9125..9949
                     /locus_tag="J1Y83_003319"
     CDS             9125..9949
                     /locus_tag="J1Y83_003319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HBA7631436.1"
                     /translation="MEQRKITRSDLVSMFLRSNLQQASFNFERIHGLGFCYDMIPAIK
                     RLYPLKEDQVAALRRHLVFFNTTPAVCGPVIGVTAAMEEARANGAEIDDGTINGIKVG
                     LMGPLAGVGDPLVWGTLRPITAALGASLALSGNILGPLLFFFIFNAVRLAMKWYGLQL
                     GFRKGVNIVSDMGGNLLQKLTEGASILGLFVMGVLVTKWTSINVPLVVSQTPAADGAT
                     VTITVQNILDQLCPGLLALGLTLLMVRLLNKKINPVWLIFALFGLGIIGNALGFLS"
     gene            10001..11230
                     /locus_tag="J1Y83_003320"
     CDS             10001..11230
                     /locus_tag="J1Y83_003320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HBA7631437.1"
                     /translation="MKTTALRLYGKRDLRLETFDLPEMQEDEILATVVTDSLCLSSWK
                     EANLGENHKKVPDDVATNPIIIGHEFCGDILAVGKKWQHKFQPGQRYVIQANLQLPDR
                     PDCPGYSFPWVGGEATHVVIPNEVMEQDCLLAYDGETYFEGSLVEPLSCVIGAFNANY
                     HLQEGSYNHTMGIRPQGRTLILGGTGPMGLLAIDYALHGPVNPSLLVITDTDNDKLSY
                     ARKHYPSEPQTLIHYLNATDAAFDTLMALSGGHGFDDIFVFVPNEGLVTLASSLLATD
                     GCLNFFAGPQDKHFSAPINFYDVHYAFTHYVGTSGGNTDDMRAAVKLIEEKKVQAAKV
                     VTHILGLNAAGETTLELPAVGGGKKLVYTGKYLPLTSLTQIQDQALAAILARHQGIWS
                     GEAEQYLLAHAEAISHD"
     gene            11612..12301
                     /gene="pepE"
                     /locus_tag="J1Y83_003321"
     CDS             11612..12301
                     /gene="pepE"
                     /locus_tag="J1Y83_003321"
                     /EC_number="3.4.13.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepE"
                     /protein_id="HBA7631438.1"
                     /translation="MELLLLSNSTLPGKAWLEHALPLIAEQLQGRRSAVFIPFAGVTQ
                     TWDDYTAKTAAVLAPLGVSVTGIHSVVDPVAAIENAEIVIVGGGNTFQLLKQCRERGL
                     LAPITDVVKRGALYIGWSAGANLACPTIRTTNDMPIVDPQGFDALNLFPLQINPHFTN
                     ALPEGHKGETREQRIRELLVVAPELTIIGLPEGNWITVSKGHATLGGPNTTYVFKAGE
                     EAVPLEAGHRF"
     gene            complement(12391..14022)
                     /locus_tag="J1Y83_003322"
     CDS             complement(12391..14022)
                     /locus_tag="J1Y83_003322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HBA7631439.1"
                     /translation="MLTLLHLLSAVALLVWGTHIVRTGVMRVFGARLRTVLSRSVEKK
                     PLAFCAGIGVTALVQSSNATTMLVTSFVAQDLVALAPALVIVLGADVGTALMARILTF
                     DLSWLSPLLIFIGVIFFLGRKQSRAGQLGRVGIGLGLILLALELIVQAVTPITQANGV
                     QVIFASLTGDILLDALIGAMFAIISYSSLAAVLLTATLTAAGIISFPVALCLVIGANL
                     GSGLLAMLNNSAANAAARRVALGSLLFKLVGSLIILPFVHLLAETMGKLPLPKAELVI
                     YFHVFYNLVRCLVMLPFVDPMARFCKTIIRDEPELDTQLRPKHLDVSALDTPTLALAN
                     AARETLRIGDAMEQMMEGLNKVMHGEPRQEKELRKLADDINVLYTAIKLYLARMPKEE
                     LAEEESRRWAEIIEMSLNLEQASDIVERMGSEIADKSLAARRAFSLDGLKELDALYEQ
                     LLSNLKLAMSVFFSGDVTSARRLRRSKHRFRILNRRYSHAHVDRLHQQNVQSIETSSL
                     HLGLLGDMQRLNSLFCSVAYSVLEQPDEDEGRDEY"
BASE COUNT         3520 a         3628 c         3757 g         3516 t
ORIGIN      
        1 aggattaacc tcacggtatg attttcgtta aatgacagac aagaggattt atctgataaa
       61 acaatgccga cttatagtcg gcattgtttt taaggctatt taatcaatcg tactatgtat
      121 taattgccga atacgatgta catatcggca tcgacctgta ggcctgataa gacgcgaccg
      181 cgtcgcatca ggcatcggtt gccggatgcg gcgtgaacgc cttatccggc ctacatatcg
      241 acaatgatta accgcgccac gctttatagc ggttaatcag accattggtc gagctatcgt
      301 ggctgctgat ttctttgtca tctttcagct ctggcagaat acggttcgcc agttgtttac
      361 ccagctccac gccccactgg tcgaaggtga agatgttcag gatcacgccc tgagtaaaga
      421 ttttgtgctc atacagcgca atcaacgccc ccaggctgaa cggagtgatt tcacgcagca
      481 ggatggagtt ggtcgggcgg ttaccttcga acactttgaa cggcaccacg tagtcaagcg
      541 ttgccggatc tttaccctga tcgcgatatt cctgctcaac cacttcgcgg gatttaccaa
      601 acgccagcgc ttcggtctga gcaaagaagt tagacagcag tttctggtga tgatcagaga
      661 gcgggttatg ggtgatagcc ggagcgatga aatcgcacgg taccattttg gttccctggt
      721 ggatcagctg atagaacgcg tgctgaccgt tcgtgccagg ttcaccccag ataatcgggc
      781 cagtctggta atccacaacc ttaccgttac ggtcaacata cttaccgttg gattccatat
      841 tgccctgctg gaagtacgcc gcgaaacggt gcatatactg gtcatacggc agaatcgctt
      901 cagtttccgc gccaaagaaa ttgttgtacc agatgccaat cagcgccagc agtacaggca
      961 ggtttttctc ggcaggcgtg gtggagaaat gcttgtccat cgcgtgtgcg ccagaaagca
     1021 gttcaacgaa gttatcaaag ccgatggaga gaacaatcga caggccaatc gctgaccaca
     1081 aagagtaacg accgccgacc cagtcccaga attcgaacat gttggcagta tcaataccaa
     1141 actcgccaac ggctttggcg ttggttgaga gcgcagcgaa gtgtttcgca acgtgtttct
     1201 catcacctgc cgctttcagg aaccagtcac gcgcgctatg ggcgttggtc atggtttcct
     1261 gagtggtgaa ggttttagat gctaccagga acagcgtggt ttccgggttt acttttttca
     1321 gcacttccgc gatgtgagtc ccatcgacgt tagaaacaaa gtgcatgttc aggtggtttt
     1381 tgtatggacg cagtgcttca gtcaccatgt acgggccgag gtcagaaccg ccgatcccga
     1441 tgttcactac gtcagtgatt gccttgccgg tatagccttt ccactcaccg gaaataatcg
     1501 cttccgagaa ggttttcatc ttctccagca ctgcgttgac ttccggcata acatctttgc
     1561 catcaaccag aatcggggta ttgctacggt tacgcagcgc tacgtgcagt acggcgcggt
     1621 tttcagtgcg gttgatcttc tcgccagaga acatcgactt aatcgcgccc gccagatcgc
     1681 actctttcgc cagatcttgt aatttcgcca gcgtctcttc agtgatgcgg tttttggagt
     1741 aatccaccag catctgatcg tcgaaggttg cggagaactt agaaaaacga tcgccgtctt
     1801 tagcaaaaag atcggcgatc gtaacgtctt tcatttcatc gaagtgtttc tgtagtgcct
     1861 gccaggcagc ggtctgcgtt ggattgatgt ttttcattag caatactctt ctgattttga
     1921 gaattgtgac tttggaagat tgtagcgcca gtcacagaaa aatgtgatgg ttttagtgcc
     1981 gttagcgtaa tgttgagtgt aaacccttag cgcagcgaag catttattag ctgaactact
     2041 gaccgccagg agtggatgaa aaatccgcat gaccccatcg ttgacaaccg ccccgctcac
     2101 cctttattta taaatgtact acctgcgcta gcgcaggcca gaagaggcgc gttgcccaag
     2161 taacggtgtt ggaggagcca gtcctgtgat aacacctgag ggggtgcatc gccgaggtga
     2221 ttgaacggct ggctacgttc atcatcggct acaggggctg aatcccctgg gttgtcacca
     2281 gaagcgttcg cagtcgggcg tttcgcaagt ggtggagcac ttctgggtga aaatagtagc
     2341 gaagtatcgc tctgcgccca cccgtcttcc gctcttccct tgtgccaagg ctgaaaatgg
     2401 atcccctgac acgaggtagt tatgtctgaa attgttgtct ccaaatttgg cggtaccagc
     2461 gtagctgatt ttgacgccat gaaccgcagc gctgatattg tgctttctga tgccaacgta
     2521 cgtttagttg tcctctcggc ttctgctggt atcactaatc tactggtcgc tttagctgaa
     2581 ggactggaac ctggcgagcg attcgaaaaa ctcgacgcaa tccgcaatat ccagtttgcc
     2641 attctggaac gtctgcgtta cccgaacgtt atccgtgaag agattgaacg tctgctggag
     2701 aatattactg ttctggcaga agcggcggcg ctggcaacgt ctccggccct gacagatgaa
     2761 ctggtcagcc acggcgagct gatgtcgacc ctgctgtttg tcgagatcct gcgcgaacgc
     2821 gatattcagg cacagtggtt tgatgtacgt aaagtgatgc gtaccaacga ccgatttggt
     2881 cgtgcagagc cagatgtagc cgcgctggcg gaactggccg cgctgcagct gctcccacgc
     2941 ctcaatgaag gcttagtgat cacccaggga tttatcggca gcgaaaataa aggtcgtaca
     3001 acgacgcttg gccgtggagg cagcgattat acggcagcct tgctggcgga ggctttacac
     3061 gcatctcgtg ttgatatctg gaccgacgtc ccgggcatct acaccaccga tccacgcgtg
     3121 gtttccgcag caaaacgcat tgatgaaatc gcgtttgccg aagcggcaga gatggcaact
     3181 tttggtgcaa aagtactgca tccggcaacg ttgctacccg cagtacgcag cgatatcccg
     3241 gtctttgtcg gctccagcaa agacccacgc gcaggtggta cgctggtgtg caataaaact
     3301 gaaaatccgc cgctgttccg cgcgctggcg cttcgtcgca atcagactct gctcactttg
     3361 cacagcctga atatgctgca ttctcgcggt ttcctcgcgg aagttttcgg catcctcgcg
     3421 cggcataata tttcggtaga cttaatcacc acgtcagaag tgagcgtggc attaaccctt
     3481 gataccaccg gttcaacctc cactggcgat acgttgctga cgcaatctct gctgatggag
     3541 ctgtctgcac tgtgccgggt ggaagtggaa gagggtctgg cgctggtcgc gttgattggc
     3601 aatgacctgt caaaagcctg cggcgttggc aaagaggtat tcggcgtact ggaaccgttc
     3661 aacattcgca tgatttgtta cggcgcatcc agccataacc tgtgcttcct ggtgcccggc
     3721 gaagatgccg agcaggtggt gcagaagctg cattttaatt tatttgagta aacacagtat
     3781 ggcccggaag acattatttc gggccgtatt gtttttcttg tcattatgcc cataaataaa
     3841 cgagcctgta ctctgttaac cagcgtcttt atcggagaat aattgccttt aattttttat
     3901 ccgcatctct aactaattat cgaaagagat aaatagttaa gagaaggcaa aatgaatatt
     3961 atcagttctg ctcgcaaacg aattcccgta cactgaactc tctggctcac tgagggcacc
     4021 atcatggcac tcccccgtat tacccaaaaa gagatgactg aacgcgagca gcgtgaactt
     4081 aaaacactgc tggatcgggc gcggattgcg catggtcgcg tactgaccaa ctccgaaact
     4141 aacagcatta agaaagagta catcgataaa ttaatggtcg agcgtgaggc tgaagcgaaa
     4201 aaagcccgcc aattgaagaa aaagcaggct tataaaccgg atcctgaggc gtcatttagc
     4261 tggtcagcaa atacgtcgac gcgcggcagg cgctgattaa cgccccttct ttttacgccc
     4321 cggcgaggta aaacgcttac cgttggacgc cgggcgaccg cctttttccg ccgttttttc
     4381 catcttaacg actggacgtt tgatgcctac cgttttcggt ttggccttcg ctttaggttt
     4441 tgcctcggaa gaggaatttt caatgagctt aaagagatcg attaactcat cgtcggttaa
     4501 atcgcgccac tcacccagcg gaatgccgct taagctgacg ttcataatgc gcgtacgttc
     4561 cagctttttc acttcgtaac caaagtgctc gcacatgcga cgaatctgac ggttcagccc
     4621 ctgcaccagg gtaatgcgga agacaaacgg tgcttctttt ttgactttgc actttttagt
     4681 tactgtcccg aggatcggta ctcccgcccc cataccacga ataaattcat cggtaatcgg
     4741 tttatcaacc gtcaccagat actctttctc atgatcgtta cccgcacgga ggatcttatt
     4801 caccagatcg ccgtgattgg tgaggaaaat cagcccctgg gaatctttat ccaggcggcc
     4861 aatcgggaac acgcgtttgc tgtggttaac gaaatcgaca atgttatcgc gctctccatc
     4921 ttcggtggtg ctgacaatac cgacgggctt gttcagggcg ataagtacca aatcttcgga
     4981 ttcccgaggc tcaatcaact gaccgtttac tttcacaacg tcgccggatt tcacctgatc
     5041 gccaatggtg gctcgcttgc cattaaggaa cacattgcct tgctcgatat agcgatccgc
     5101 ttcgcggcgt gagcaaattc cgctttcgct gatgtattta tttaaacgga ctgatgagtc
     5161 gggcagcata aattctcctg taaaacgcgg aatataccgt acctcttagc ggggaaaaac
     5221 agggttatgc ggcaacgtcg aaattaaatg tgatctgcca cgccttttat cacaaaagtg
     5281 tctccggaag tacgatgcaa atttgcctgg caaaataaga ataatcaaca aattacgaat
     5341 cgctgcacac ctgtgctgat gactactacg cagagaaaca aatgtgcaag agaaagcgct
     5401 atttcccccc atgcaaagag tgaaaaatcg ctttgaaagg ccatcatgat ggggttatat
     5461 cagatcaagc ggtgagaaag cgtatttgca caaatgtgca gaaccaggag gcggcaatgg
     5521 aaaacagtga cgatatccgt ttgattgtga agattgccca actctattac gaacaggata
     5581 tgacgcaggc gcaaattgcg cgcgaactgg gtatttaccg caccaccatc agccgcttgc
     5641 ttaaacgagg ccgcgatcag ggaattgtca ccatcgccat caactatgac tacaacgaaa
     5701 atctctggct ggagcagcaa gtgaagcaaa agtttggcct gaaagacgtt gtggtggtgt
     5761 caggaaatga tgaggatgaa gatactcaac tggcgatgat ggggttacac ggcgcgcaac
     5821 tgctggatcg cttgctggaa cctggcgata ttgtcggttt ttcctggggc cgcgcggtga
     5881 gcgcactggt tgaaaacttg ccgcaggcgg ggcaatcgcg gcagttaatt tgcgtgccga
     5941 ttattggtgg cccgtccggt aaactcgaaa gccgctatca cgtaaacaca ttaacctaca
     6001 gcgcggcagc gaagctgaaa ggggaatcgc atctcgcgga ttttccggct ctgctggata
     6061 acccattaat tcgtaatggg atcatgcagt ctcagcactt taaaaccatc tctgcctact
     6121 gggataatct ggatgtcgcc ctggtgggaa ttggctcacc ggccattcgc gacggcgcta
     6181 actggcatgc gttttatggt ggtgaagaga gtgacgacct gaatgcccgc caggttgctg
     6241 gcgatatttg ctcgcgcttt tttgatattc actgcgcaat ggttgaaacg aatatgagcg
     6301 aaaaaacact ctctatcgaa atgaataaat taaagcaggc acggtattcc attggcattg
     6361 ccatgagcga agaaaaatac agcggaattg ttggtgcact gcgtggaaaa tatattaatt
     6421 gtctggtaac gaatagcagc acagctgaac tattactgaa ataaaaatct tcccgatgtt
     6481 taccgtaata aaaattacgg cgggtcccta ctatctaaaa tcaggagtct gttatgcaaa
     6541 cgtggttaaa tttgcaggat aaaatcatta ttgtcaccgg tggcgcatcc ggtattggtc
     6601 tggcgattgt ggatgaatta ttagcacaag gcgcgaatgt acagatggtc gatattcact
     6661 gtggcgatgg tcagtatgaa agccataaag gttatcagtt ctggccgacc gatatttcca
     6721 gcgccaaaga gataaatcat acggtagcag aaattatcca gcgttttggt cgcatcgacg
     6781 gtctggttaa caacgccggg gtcaatttcc cgcgtctgct ggtcgatgag aaagcgcctg
     6841 ccgggcagta tgaactcaac gaagctgcat tcgaaaaaat ggtcaatatc aaccagaaag
     6901 gcgtttttct gatgtcgcag gcggtggcgc gacagatggt caaacaacat gatggcgtga
     6961 ttgtgaatgt ttcctcagaa agtgggctgg aaggctcaga aggccaaagc tgttacgccg
     7021 cgaccaaagc cgcgctcaat agcttcacgc gctcctggtc gaaagaactg ggtaagcacg
     7081 gtatccgtgt ggtcggtatc gcgccgggga ttctggaaaa aacaggactg cgcacgccgg
     7141 aatatgaaga agcgctggcg tggacgcgca atatcaccgt cgagcagctg cgtgaaggct
     7201 ataccaaaaa cgccattcct attgggcgcg ccggaagatt agcagaagtg gctgattttg
     7261 tttgttatct gctgtctgaa cgcgccagct atatcaccgg agtaaccact aacattgcag
     7321 gcggcaaaac gcgcggctaa ggaggcttta tggtcaatgc cattttttgc gcccacggca
     7381 aattagcctg cgccatgctg gaatcggttc agatggtgta cggcgatgcc agggtcgagg
     7441 ccgttgaatt tgtgcccggc gagaacgccg gagacattgt ggcaaagctg gagaagttag
     7501 taagcattca caatcaagat gagtggctaa tcgccgtgga tttacagtgc ggcagcccgt
     7561 ggaatgcggc agcgatgctt gcgatgcgca atcctcgtct gcgggtaatc agcggccttt
     7621 ctctgccgct ggcgctcgaa ctggttgata accaggacag catgaatatt gatgaactgt
     7681 gtgaacacct gacgcaaatc gcaaagcaaa cctgcgtcgt ctggagacag gtggcaacag
     7741 cggaggagga tttctgatga acattacgct cgcccgcatt gatgaccgcc tgatccacgg
     7801 ccaggtcact accgtctggt ctaaagtggc taacgcccag cgcattatta tctgcaatga
     7861 cgaagtttat aacgatgaag ttcgccggac attattgcgt caggctgcgc caccgggaat
     7921 gaaggttaac gtggtcaata ttgaaaaagc cgtcgcggtt tatcacaacc cgcaatatca
     7981 ggacgaaacc gttttttatc ttttcactcg cccacaggat gccttagcga tggttcgcca
     8041 gggggtaaaa ataggcaccc tgaatattgg cggcatggcc tggcggcccg gtaaaaaaca
     8101 gttaaccaaa gcggtttcat tagatgatga tgatattaac gcatttcatg agttaaataa
     8161 tcttggcatc atactggatt tacgcgtcgt agcgtcagat ccctcaatca atattatcga
     8221 caaaataaac gaacagttaa tcgcaaatta aataatagcg cctgtgtaca tatgctttaa
     8281 caggcaggga tgacgacact taaaaggtga caaatcatgg aaataagtac cctacaaatc
     8341 attgctatat ttcttttttc ctgtattgcc ggaatgggca gcgtgctgga tgaatttcag
     8401 actcatcgcc cgttaattgc ctgtacggtg attggtttaa ttctcggtga tttaaaaacc
     8461 ggaattatgc tcggtggtac gctggaattg atagctctcg gctggatgaa cgtcggcgcg
     8521 gcgcaatctc cggattctgc actcgccagc ataatctccg ccattctggt tatcgttggt
     8581 cagcaaagca tcgccaccgg aatcgccatc gcgttgcctg tggctgcggc aggccaggtg
     8641 ctgacggtgt ttgcccgtac catcaccgta gtgttccagc acgcggcgga taaagcagcg
     8701 gaagaagcac ggtttcgcac tctcgatatt ctgcatgtct ccgcgcttgg cgtgcaggca
     8761 ctgcgcgttg ctattccggc actgattgtc tcgctgttcg tcagcgctga tatggtgagc
     8821 aatatgctga gcgccattcc cgaatttgtg acccgtggac tgcagattgc tggcggtttt
     8881 atcgtggtgg tcggttacgc catggtgctt cgcatgatgg gcgtgaaata tttgatgcct
     8941 ttctttttcc tcggtttcct cgcaggtggc tacctcgatc tcagtctgct ggcgttcggt
     9001 ggcgtcggcg tgatcatggc cctgctctac atccagttaa atccacagtg gcgtaaagct
     9061 gaaccacatc cccagaccac cactatcacc gcccttgacc aacttgatga ttaacggagc
     9121 catcatggaa cagagaaaaa ttacacgcag cgatctggtg agcatgtttc tgcgctccaa
     9181 cctgcaacag gcatccttta actttgaacg tattcacggg ctgggttttt gctacgacat
     9241 gatccccgcc atcaaacgac tttacccatt aaaagaggat caggttgcgg cgctcaggcg
     9301 acacctggtg ttcttcaata ccacgccagc cgtatgtggc ccggtcatcg gcgtcactgc
     9361 tgccatggaa gaggcgcggg ccaacggcgc ggaaattgat gacggtacca ttaacggcat
     9421 caaagtcggt ctgatgggac cattggcagg agttggcgat ccactggtct ggggaacgct
     9481 gcgcccgatt accgccgcgc tcggcgcatc tctggcactt tcgggcaaca ttctcggccc
     9541 cctgctgttc ttctttattt tcaacgcggt gcgtctggcg atgaagtggt atggcctaca
     9601 gctcggcttt cgcaaagggg tgaatatcgt cagcgatatg ggcggtaatt tgctgcaaaa
     9661 actcaccgaa ggcgcgtcga ttctcgggct gtttgtgatg ggcgtgctgg tgaccaaatg
     9721 gacgtcaatc aacgtaccgt tggtggtttc acaaacgcct gccgccgatg gtgccaccgt
     9781 caccataacc gtgcagaaca tcctcgacca actttgccct ggtttgctgg cgctcggtct
     9841 gacgctgctg atggttcgtc tgctcaacaa aaaaattaac ccggtatggc tgattttcgc
     9901 cctgtttggt ttagggatta tcggtaatgc gctgggcttc ctgtcctgat tcttcgcccc
     9961 ggcgcaaccg tcggggccat cgttcaacat gaggtggttt atgaaaacaa cagctctgcg
    10021 tctttatggc aaacgtgacc tgcgtctgga aacctttgac cttcctgaaa tgcaggagga
    10081 tgaaatcctc gcaacggtgg tcactgacag cctgtgcctc tcttcctgga aagaggccaa
    10141 tctgggtgaa aaccataaaa aagtacccga cgatgtggcg accaatccca tcatcatcgg
    10201 ccacgaattt tgcggcgata ttctggccgt gggtaaaaag tggcagcaca aattccagcc
    10261 gggtcagcgt tatgtgattc aggccaacct gcaactcccc gaccgcccgg attgccccgg
    10321 ctactccttc ccgtgggtag gcggcgaagc gacgcatgtg gttattccca acgaggtcat
    10381 ggaacaagat tgcctgctgg catacgacgg cgaaacctat tttgaaggct cgctggttga
    10441 accgctttcc tgcgtgattg gcgcgttcaa cgccaactat catcttcagg aaggtagtta
    10501 taaccacacg atggggattc gcccgcaagg gcgcacgctg atcctcggcg gcaccggccc
    10561 gatggggctg ctggcgattg attatgcgct acatggaccc gttaaccctt cgctgctcgt
    10621 cattaccgat accgacaacg acaaattgag ttatgcgcgc aagcactatc catcagaacc
    10681 gcaaacactg attcattatc tcaatgccac cgatgcagca tttgatacgt taatggcact
    10741 gagtggcggt cacggcttcg atgatatttt cgtctttgtg cctaatgaag gactggtgac
    10801 tctcgcctct tccttgctgg cgacagatgg ttgtctgaat ttcttcgccg gaccgcagga
    10861 taagcacttc agcgcgccaa ttaatttcta cgatgtgcat tatgcattta cccactacgt
    10921 gggcacatca ggcggcaata ccgacgacat gcgcgcagcg gtcaaattga ttgaagagaa
    10981 aaaagtgcag gccgcaaaag tggtaacaca tattcttggg ctgaatgccg cgggcgaaac
    11041 cacgcttgaa ttgcctgccg tcggcggcgg caaaaagctg gtgtataccg ggaaatacct
    11101 gccgctgacg tcactcacgc agattcagga tcaagcactg gcggcgattc tggcgcgtca
    11161 tcaggggatc tggtccggtg aggcggagca atacctgctc gcccatgcag aggcaatttc
    11221 ccatgattaa tcgcgctact taactctgca tgtcgctggc tggtcgccgc ggcaactttg
    11281 cgacctgatg tgcggctgat tgctgaagcg gcacaatgtg cacgagaagg ataaaaaaac
    11341 atcagcgcct taagaggtgc tcaaattggt ggcaaagtgc acattgttta tgccggatgc
    11401 ggaatgaagc cttattcggc ctacataatc ttgaaaattc aatatattgc agttatctgg
    11461 taggccaggt aagcgtagcg catcaggcaa tcctgtgttt gtcatcagtc tcagcgcctg
    11521 tgcaatatgt gacatcgtga gccatgtcag taagtggtta aattcgcagt ggtagcattc
    11581 cttccgtgtt ttcttactaa ccggagaagt aatggaactg cttttattga gtaactcgac
    11641 gctgccgggt aaagcctggc tggaacacgc gctgccgctg attgctgaac agttgcaggg
    11701 tcgccgttct gcggtattta ttcctttcgc tggcgtaacg cagacctggg atgattacac
    11761 agcgaaaacg gctgcggttc tcgctccgct gggtgtttct gtcaccggta ttcatagcgt
    11821 tgtcgatccc gttgccgcga ttgaaaatgc ggaaattgtg attgtcggcg gcggtaatac
    11881 tttccagttg ctgaaacagt gccgcgagcg cgggctgctg gcaccaatta ctgacgtggt
    11941 gaaacgtggc gcgctgtata ttggctggag cgcaggcgct aaccttgctt gcccaaccat
    12001 tcgtaccacc aacgatatgc cgattgtcga tccgcaaggt ttcgatgcgc taaatctgtt
    12061 cccgctgcaa atcaacccgc acttcaccaa cgcgctgccg gaaggccata aaggtgaaac
    12121 ccgtgagcag cgtattcgcg aactgctggt cgtcgcgcca gaactgacga ttattggtct
    12181 gccggaaggt aactggatca cagtaagtaa aggtcacgct acgctgggtg gcccgaacac
    12241 cacttatgta tttaaggctg gtgaagaagc ggtgccgctg gaagcgggtc accgttttta
    12301 agtgaattgc cggatacatg atccggcatc ttgttcatag tgcctgatgc gacgctttac
    12361 gcgtcttatc aggccttcag ggtcacggta ttaatactcg tcccgtcctt catcttcatc
    12421 cggctgttcc agcacactgt aagccaccga acaaaacagc gagttcagac gctgcatatc
    12481 tcccagtaag cctaaatgta gcgaactggt ttcaatactc tgcacgtttt gctggtgcag
    12541 gcgatcgacg tgggcgtggg aatagcggcg attaagaatg cgaaaacgat gtttgctgcg
    12601 gcgcaaacga cgggcgctgg taacatcgcc agagaagaaa accgacattg ccagttttaa
    12661 attactgagc aattgctcat agagcgcatc cagttccttc aagccatcaa gcgaaaatgc
    12721 tcgccttgct gccagcgatt tgtcggcaat ttcactcccc atgcgctcga cgatatcgga
    12781 ggcctgttca aggttgagcg acatttcgat gatctccgcc cagcggcgcg actcttcttc
    12841 tgccagttcc tcttttggca tccgcgccag atacagctta atggcggtat agagaacgtt
    12901 gatgtcatca gccagcttac gcagctcttt ctcctgccgt ggctcgccgt gcatcacttt
    12961 attcaaccct tccatcatct gttccatggc gtcgccaatg cgcagggttt cgcgggcagc
    13021 gttcgccaga gcaagcgtgg gcgtatccag cgcgctgaca tccagatgtt taggccgtag
    13081 ctgggtatcc agttccggtt catcgcgaat aatcgttttg caaaaccgtg ccatcgggtc
    13141 aacgaatggc aacatgacca ggcagcgtac aaggttgtag aagacgtgga aatagatcac
    13201 caattccgct tttggcaatg gcaacttccc cattgtctct gccagcaaat gaacaaacgg
    13261 caggataatc aggctaccca ccagcttaaa cagcaggcta cccagcgcga cacggcgggc
    13321 tgcggcattg gcggcactgt tgttgagcat cgccagcagg ccggaaccga ggtttgcacc
    13381 aatcaccaga caaagcgcca ctgggaaaga gataatgcct gcggcggtca gagtcgcggt
    13441 cagcagtacc gcggcaaggc tggagtagct gataatggcg aacatcgcac caatcagcgc
    13501 atccagcaaa atgtcgccag tcagcgaggc aaagatcacc tgaacgccgt ttgcctgagt
    13561 gatcggcgtt acggcctgca caatcaactc cagcgccagt aaaatcagcc caagaccaat
    13621 accgacgcgg cccagctgcc cggcgcgtga ctgtttgcgt ccgaggaaaa aaatcacgcc
    13681 gataaaaata agtaacggtg acagccagga taaatcgaag gtgagaatac gcgccatcag
    13741 cgccgtcccg acatctgcac ccagcacaat caccagcgca ggcgcgagag caaccagatc
    13801 ctgagcgacg aacgaggtca ccagcatggt ggtggcatta ctgctctgca ccagtgcggt
    13861 aacgccgatc cccgcgcaaa aggcgagcgg ctttttttcg acgctccggc taaggacagt
    13921 acgcagacgc gcgccgaaga cgcgcattac gcctgttcga acaatatgag tcccccagac
    13981 cagcagggcg acggcagaaa gcaggtgaag caaagttagc acggaatcag gttctccttt
    14041 ttgttatgca ttttcctgtt aagatttttc taatcacgtt gtttttatcc aggcatttct
    14101 cttgtatgta ctttcagtat aagagattta tacaagaaag catttaaaaa ggcatttata
    14161 taaaaatcca cctgtatctg ttgctatttc tgtcacactt tcccctgtcg tttacaccag
    14221 ataatcgttg tcacttttgt gacctctgag aagataaaca acgaaatatt taagaccaga
    14281 ttctgtgaat gaaaataatt aagtaacaac ggtattacgg agattgagca gcttacggta
    14341 gcaaaacgca taacttcctg aatcgccaaa aaagagaaac taaataattt ttgtcttaaa
    14401 aaacattgcg cagaaaagaa t
//
LOCUS       DADSYM010000124        13910 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2415
            SAMN08039579-rid11723173.denovo.125, whole genome shotgun sequence.
ACCESSION   DADSYM010000124 DADSYM010000000
VERSION     DADSYM010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039579
            Sequence Read Archive: SRR6362533
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13910)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13910)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980162.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 03:39:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,484
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 4,347
            CDSs (with protein)               :: 4,347
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 43 of 85
            Pseudo Genes (incomplete)         :: 37 of 85
            Pseudo Genes (internal stop)      :: 19 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13910
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039579-rid11723173.denovo.125"
                     /strain="LGE2415"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8302 N 95.9628 W"
                     /collection_date="2015-08-24"
     gene            complement(2..1846)
                     /gene="selB"
                     /locus_tag="J1Y83_003323"
     CDS             complement(2..1846)
                     /gene="selB"
                     /locus_tag="J1Y83_003323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenocysteine-specific translation elongation
                     factor"
                     /protein_id="HBA7631440.1"
                     /translation="MIIATAGHVDHGKTTLLQAITGVNADRLPEEKKRGMTIDLGYAY
                     WPQPDGRVPGFIDVPGHEKFLSNMLAGVGGIDHALLVVACDDGVMAQTREHLAILQLT
                     GNPMLTVALTKADRVDEARVDEVERQVKEVLREYGFAEAKLFITAATEGRGIDALREH
                     LLQLPEREHASQHSFRLAIDRAFTVKGAGLVVTGTALSGEVKVGDSLWLTGVNKPMRV
                     RALHAQNQPTETAHAGQRIALNIAGDAEKEQINRGDWLLADVPPEPFTRVIVELQTHT
                     PLTQWQPLHIHHAASHVTGRVSLLEDNLAELVFDTPLWLADNDRLVLRDISARNTLAG
                     ARVVMLNPPRRGKRKPEYLQWLASLARAQSDADALSVHLERGAVNLADFAWARQLNGE
                     GMRELLQQPGYIQAGYSLLNAPVAARWQRKILDTLATYHEQHRDEPGPGRERLRRMAL
                     PMEDEALVLLLIEKMRESGDIHSHHGWLHLPDHKAGFSAEQQAIWQKAEPLFGDEPWW
                     VRDLAKETGTDEQAMRLTLRQAAQQGIITAIVKDRYYRNDRIVEFANMIRDLDQECGS
                     TCAADFRDRLGVGRKLAIQILEYFDRIGFTRRRGNDHLLRDALLFPEK"
     gene            complement(1843..3234)
                     /gene="selA"
                     /locus_tag="J1Y83_003324"
     CDS             complement(1843..3234)
                     /gene="selA"
                     /locus_tag="J1Y83_003324"
                     /EC_number="2.9.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-seryl-tRNA(Sec) selenium transferase"
                     /protein_id="HBA7631441.1"
                     /translation="MTTETRSLYSQLPAIDRLLRDSSFLSLRDTYGHTRVVELLRQML
                     DEAREVIRDSQTLPAWCENWAQEVDARLTKEAQSALRPVINLTGTVLHTNLGRALQAE
                     AAVEAVTKAMRSPVTLEYDLDDAGRGHRDRALAQLLCRITGAEDACIVNNNAAAVLLM
                     LAATASGKEVVVSRGELVEIGGAFRIPDVMRQAGCTLHEVGTTNRTHAKDYRQAVNEN
                     TALLMKVHTSNYSIQGFTKAIDEAELVALGKELDVPVVTDLGSGSLVDLSQYGLPKEP
                     MPQELIAAGVSLVSFSGDKLLGGPQAGIIVGKKEMIARLQSHPLKRALRADKMTLAAL
                     EATLRLYLHPEALSEKLPTLRLLTRSAEVIQIQAQRLQAPLAAHYGAEFAVQVMPCLS
                     QIGSGSLPVDRLPSAALTFTPHDGRGSHLESLAARWRELPVPVIGRIYDGRLWLDLRC
                     LEDEQRFLEMLLK"
     gene            complement(3332..3940)
                     /locus_tag="J1Y83_003325"
     CDS             complement(3332..3940)
                     /locus_tag="J1Y83_003325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase"
                     /protein_id="HBA7631442.1"
                     /translation="MKLVGSYTSPFVRKLSILLLEKGITFEFINELPYNADNGVAQFN
                     PLGKVPVLVTEEGECWFDSPIIAEYIELMNVAPAMLPRDPLESLRVRKIEALADGIMD
                     AGLVSVREQARPAAQQSEDELLRQREKINRSLDVLEGYLVDGTLKTDTVNLATIAIAC
                     AVGYLNFRRVAPGWCVDRPHLVKLVENLFSRESFARTEPPKA"
     gene            complement(4011..5147)
                     /locus_tag="J1Y83_003326"
     CDS             complement(4011..5147)
                     /locus_tag="J1Y83_003326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HBA7631443.1"
                     /translation="MDLLIVLTYVALAWAVFKIFRIPVNQWTLATAALGGVFLVSGLI
                     LLMNYNHPYTFTAQKAVIAIPITPQVTGIVTEVTDKNNQLIQKGEVLFKLDPVRYQAR
                     VDRLQADLMTATHNIKTLRAQLTEAQANTTQVSAERDRLFKNYQRYLKGSQAAVNPFS
                     ERDIDDARQNFLAQDALVKGSVAEQAQIQSQLDSMVNGEQSQIVSLRAQLTEAKYNLE
                     QTVIRAPSNGYVTQVLIRPGTYAAALPLRPVMVFIPEQKRQIVAQFRQNSLLRLKPGD
                     DAEVVFNALPGQVFHGKLTSILPVVPGGSYQAQGVLQSLTVVPGTDGVLGTIELDPNA
                     DIDALPDGIYAQVAVYSDHFSHVSVMRKVLLRMTSWMHYLYLDH"
     gene            complement(5150..5512)
                     /locus_tag="J1Y83_003327"
     CDS             complement(5150..5512)
                     /locus_tag="J1Y83_003327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3302 domain-containing protein"
                     /protein_id="HBA7631444.1"
                     /translation="MFLNYFALGVLIFVFLVIFYGIIAIHDIPYLIAKKRNHPHADAI
                     HTAGWVSLFTLHVIWPFLWIWATLYQPERGWGMQSHVTPQEKTTDAEIAALSDRISRL
                     EHQLAAEKKTDYSTFPEI"
     gene            6049..7962
                     /locus_tag="J1Y83_003328"
     CDS             6049..7962
                     /locus_tag="J1Y83_003328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICBA"
                     /protein_id="HBA7631445.1"
                     /translation="MSSDIKIKVQSFGRFLSNMVMPNIGAFIAWGIITALFIPTGWLP
                     NETLAKLVGPMITYLLPLLIGYTGGKLVGGERGGVVGAITTMGVIVGADMPMFLGSMI
                     AGPLGGWCIKHFDRWVDGKIKSGFEMLVNNFSAGIIGMILAILAFLGIGPVVEALSKM
                     LAAGVNFMVVHDMLPLASIFVEPAKILFLNNAINHGIFSPLGIQQSHELGKSIFFLIE
                     ANPGPGMGVLLAYMFFGRGSAKQSAGGAAIIHFLGGIHEIYFPYVLMNPRLILAVILG
                     GMTGVFTLTILGGGLVSPASPGSILAVLAMTPKGAYFANIAGVCAAMAVSFVVSAILL
                     KTSKVKEEDDIEAAARRVQEMKAESKGASPLSAGDVTNDLSHVRKIIVACDAGMGSSA
                     MGAGVLRKKIQDAGLSQISVTNSAINNLPPDVDLVITHRDLTERAMRQVPQAQHISLT
                     NFLDSGLYTSLTERLVAAQRHTENEVKVKDSLKDSFDDSSANLFKLGAENIFLGRKAA
                     TKEEAIRFAGEQLVKGGYVEPEYVQAMLDREKLTPTYLGESIAVPHGTVEAKDRVLKT
                     GVVFCQYPEGVRFGEEEDDIARLVIGIAARNNEHIQVITSLTNALDDESVIERLAHTT
                     SVDEVLELLAGRK"
     gene            8281..9429
                     /gene="mtlD"
                     /locus_tag="J1Y83_003329"
     CDS             8281..9429
                     /gene="mtlD"
                     /locus_tag="J1Y83_003329"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HBA7631446.1"
                     /translation="MKALHFGAGNIGRGFIGKLLADAGIQLTFADVNQVVLDALNARH
                     SYQVHVVGETEQVDTVSGVDAVSSIGDDVVDLIAQVDVVTTAVGPVVLERIAPAIAKG
                     LVKRKEQGNESPLNIIACENMVRGTTQLKGHVMNALPEDAKAWVEEHVGFVDSAVDRI
                     VPPSASATNDPLEVTVETFSEWIVDKTQFKGTLPNIPGMELTDNLMAFVERKLFTLNT
                     GHAITAYLGKLAGHQTIRDAILDEKIRAVVKGAMEESGTVLIKRYGFDADKHAAYIQK
                     ILGRFENPYLKDDVERVGRQPLRKLSAGDRLIKPLLGTLEYGLPHKNLIEGIAAAMHF
                     RSEDDPQAQELAALIADKGPQAALAEISGLDANSEVVSEAVTAYKAMQ"
     gene            9429..10016
                     /gene="mtlR"
                     /locus_tag="J1Y83_003330"
     CDS             9429..10016
                     /gene="mtlR"
                     /locus_tag="J1Y83_003330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor MtlR"
                     /protein_id="HBA7631447.1"
                     /translation="MVDQAQDTLRPNNRLSDMQATMEQTQAFENRVLERLNAGKTVRS
                     FLITAVELLTEAVNLLVLQVFRKDDYAVKYAVEPLLDGDGPLGDLSVRLKLIYGLGVI
                     NRQEYEDAELLMALREELNHDGNEYAFTDDEILGPFGELHCVAALPPPPQFEPADSSL
                     YAMQIQRYQQAVRSTMVLSLTELISKISLKKAFQK"
     gene            complement(10027..10236)
                     /locus_tag="J1Y83_003331"
     CDS             complement(10027..10236)
                     /locus_tag="J1Y83_003331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA7631448.1"
                     /translation="MGKLGENVPLLIDKAVDFMASSQAFREYLKKLPPRNAIPSGIPD
                     ESVPLYLQRLEYYRQLYRPKQVEEK"
     gene            10520..10882
                     /locus_tag="J1Y83_003332"
     CDS             10520..10882
                     /locus_tag="J1Y83_003332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibL family ribosome-associated protein"
                     /protein_id="HBA7631449.1"
                     /translation="MKEVEKNEIKRLSDRLDAIRHQQADLSLVEAADKYAELEKEKAT
                     LEAEIARLREVHSQKLSKEAQKLMKMPFQRAITKKEQADMGKLKKSVRGLVVVHPMTA
                     LGREMGLQEMTGFSKTAF"
     gene            11441..12124
                     /locus_tag="J1Y83_003333"
     CDS             11441..12124
                     /locus_tag="J1Y83_003333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001049680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3251 domain-containing protein"
                     /protein_id="HBA7631450.1"
                     /translation="MNLKKTLLSVLMILQLCLLVGCDYIEKASKVDDLVTQQELQKSK
                     IEALEKQQELDKRKIEHFEKQQTTIINSTKTLAGVVKAVKDKQDEFVFTEFNPAQTQY
                     FILNNGSVGLAGKILSIDAVENGSVIRISLVNLLSVPVSNMGFYATWGGEKPTDINAL
                     AKWQQLLFSTSMNSSLKLLPGQWQDINLTLKGVSPNNLKYLKLAINMANIQFDRLQPA
                     ESPQRKNKK"
     gene            12168..>13910
                     /locus_tag="J1Y83_003334"
     CDS             12168..>13910
                     /locus_tag="J1Y83_003334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001297990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adhesin"
                     /protein_id="HBA7631451.1"
                     /translation="MNKIFKVIWNPATGSYSVASETAKSRGKKSGRSKLLISALIVTS
                     AIFTPGAYAGLSLDGGDIFESTPLTNYWLAIGQGSVATTNNGGTDGVAMAIGLKAKAL
                     GAGSTAFGYDAEASGDRAIAFGQLTEASGNRTIAMGSGATATGDHSLALGGATKTLGM
                     YSVAIGRDATTDSDYALSMGHMAKANGLYSLAMGAGSATSNDNAIAIGKKTQAQGVNS
                     IALGNASQASGYSSLAIGELSETGAENAIALGKLSNASKINSIALGSNSTASGEGSVA
                     LGENSFAGGINSLALGSQSNANGDNAVALGVGSVAAQDNTVSVGNSTTQRKITNMAAG
                     QIRNGSTEAINGSQLYGLSDSVAARLGGGAGVNEDGSINAPSYKLKSNIYNNVGDALL
                     GIDNDTLHWDKTNKAFSASYLAKNADGSLKERSDQNKIINVAKGTISATSTDVVNGSQ
                     LYDLQQDALLWNGTAFSAAHGSDTTSKITNVAKGTVSATSTDVVNGSQLYDLQQDALL
                     WNGTAFSAAHGTEATSKITNVEDGTISDTSKDAVNGSQLKETKDDVATNTANIADNTA
                     DIATNTTNIADNTAD"
BASE COUNT         3578 a         3627 c         3466 g         3239 t
ORIGIN      
        1 cttatttttc cggaaataat aatgcgtcgc gcaataaatg atcatttcca cgacgacgcg
       61 taaagccaat acggtcaaaa tattccagaa tctgaattgc cagcttgcgg cctacgccta
      121 agcgatcgcg gaaatccgcc gcgcaggttg aaccgcattc ctgatcgaga tcgcggatca
      181 tattggcaaa ttcgacaatc cgatcgttac ggtaataacg atctttgacg atcgcagtaa
      241 ttatcccttg ttgcgccgcc tggcgtagcg tcaggcgcat tgcctgctcg tcggttcccg
      301 tctcttttgc caggtcacgc acccaccatg gctcgtcacc aaacagcggc tctgcttttt
      361 gccagatggc ctgctgctct gcactgaagc ccgctttatg atccggcaga tgcagccagc
      421 cgtgatggct gtggatgtcg ccgctttcgc gcatcttttc aatcagcaac agtaccagcg
      481 cttcatcttc cattggcaac gccatacgtc gcagacgttc gcgcccagga ccaggttcat
      541 cgcgatgttg ctcatgataa gtcgctaatg tgtcgagaat tttccgctgc cagcgggcgg
      601 caaccggcgc attcaacaag ctataaccag cctgaatata gccaggctgt tgcagcaatt
      661 cgcgcatccc ttcgccgttg agctgccgtg cccaggcgaa atccgcaagg ttaaccgcgc
      721 cgcgttccag atgaacagat aacgcatcgg catcgctctg cgcccgtgca agagatgcca
      781 gccattgcag atattccggc ttacgcttac cgcgacgcgg cgggttaagc atcaccacac
      841 gcgcccccgc cagcgtgttg cgggccgaga tatcgcgcag cactaagcga tcgttatccg
      901 ccagccacag cggcgtgtcg aataccagct cggcaaggtt atcttccagc agcgaaacgc
      961 gtcccgtgac gtggctggcg gcgtggtgaa tatgcagcgg ctgccactgg gtcagcggtg
     1021 tatgggtttg aagctcgaca atcacccgtg tgaacggctc cggcggcaca tcggcaagca
     1081 accagtcgcc acggttaatc tgctcttttt ccgcatcacc cgcgatgtta agcgcgatac
     1141 gctgcccggc atgggcagtt tctgttggct gattttgcgc atgcagcgca cgcacgcgca
     1201 tcggtttatt cacaccagtc agccagagtg aatcgcctac tttcacttcc ccgcttaacg
     1261 ccgtaccggt gacgaccagc ccggcacctt ttacggtaaa tgcgcggtcg atcgcgaggc
     1321 ggaaactatg ttggctggcg tgttcgcgtt ctggtaactg aagcagatgc tcgcgcaggg
     1381 catcaattcc ccgaccttcg gttgctgcgg tgataaacag ttttgcctca gcaaaaccgt
     1441 attcccgcag cacctccttt acctggcgtt caacctcatc aacacgcgct tcgtccacgc
     1501 gatcggcttt ggtcagcgcc actgtcagca tcgggttacc ggtcagctgc aaaatcgcca
     1561 gatgctcacg ggtctgtgcc atcacaccgt catcgcacgc caccaccaac agcgcgtgat
     1621 cgataccacc aacgcccgcc agcatgttgg aaagaaactt ttcatgaccg ggtacgtcga
     1681 taaaaccagg cacgcgacca tccggctgcg gccagtaggc atagccgaga tcgatggtca
     1741 tgccgcgctt tttttcttcc ggcagacggt cggcatttac gcccgtaatc gcctgcaaca
     1801 aggttgtttt gccgtggtca acgtgtccgg cagtcgcaat aatcatttca acaacatctc
     1861 caaaaaacgt tgctcatctt caaggcagcg taaatccagc cacaatcgtc cgtcataaat
     1921 acgaccaatc accggcactg gcaattcacg ccagcgggcg gctaatgact caaggtggct
     1981 accgcgtcca tcatgggggg taaacgttaa tgccgcgctc ggcaggcgat caaccggcag
     2041 cgaaccactg ccgatctgcg aaagacatgg cataacctgt accgcaaact ccgcgccgta
     2101 atgtgcggca aggggagcct gtaaacgttg tgcctggatt tgaatgacct ctgcgctgcg
     2161 ggtaagcagg cgcagggtcg gtaatttttc actcagagct tcagggtgta aataaagacg
     2221 caacgtggct tccagcgccg cgagggtcat tttatccgcg cgtaatgcac gcttcagcgg
     2281 gtggctttgc aggcgggcga tcatctcttt tttaccaaca ataattcctg cctgcggccc
     2341 gcctaacagc ttgtcgccgg agaagctcac cagactgacg cccgccgcaa tcaactcctg
     2401 cggcattggc tctttcggca aaccgtactg gctaagatcg accagcgagc cactgcctaa
     2461 atcagtcacc actggaacat ccagttcttt gccgagcgcc accagttccg cttcatctat
     2521 cgctttggtg aacccctgaa tgctgtagtt actggtatgt actttcatca acagtgcggt
     2581 attttcattc accgcctgac gataatcctt cgcgtgcgta cggttggtgg tccctacttc
     2641 gtgtagggtg catcctgcct gacgcataac atcgggaata cgaaacgcgc cgccaatctc
     2701 caccagttcg ccgcgagaaa ccaccacctc tttcccgctg gcagtggccg ccaacatcaa
     2761 taacaccgcc gccgcattgt tattaacgat acaggcatct tccgcccccg taatacggca
     2821 cagcagctgc gccagcgccc gatcgcgatg tccgcgtccg gcgtcgtcca gatcgtactc
     2881 gagggtcact ggcgaacgca tagccttcgt aacggcttcc accgcggctt ccgcctgtaa
     2941 agctcgccca aggttggtat gcagcacggt tcccgtcagg ttgatcaccg gacgcagcgc
     3001 gctttgcgcc tctttcgtca accgggcatc gacttcttgc gcccagtttt cacaccacgc
     3061 gggcagcgtc tggctgtcac gaatcacttc tcgcgcttcg tcaagcatct gacgcagcaa
     3121 ttccaccacg cgggtgtgac cataagtatc acgcaaagaa aggaaggagc tatcgcgcaa
     3181 taagcgatca atagccggaa gttgactata gagggaacgc gtttcggttg tcataggaaa
     3241 cctggctgat caaggccctc tcacacggag aagggcgttt aacataacca cggattgtaa
     3301 cgtgagatgg gtcaggagga catatcgcgc atcaagcctt tggcggttcg gtgcgagcaa
     3361 aactttcgcg gctaaacagg ttttcgacca gtttgactaa gtgtgggcgg tcgacacacc
     3421 agcctggcgc aacgcggcgg aaattgagat atccgacggc acaggcaatg gcgatagtcg
     3481 ccagattgac cgtatcggtt ttgagtgtgc catcgaccag atatccttcc agcacatcca
     3541 gactgcggtt gattttctca cgctgacgta acaattcatc ttcagactgc tgcgccgccg
     3601 gacgcgcctg ttcacgcacc gataccagcc cggcatccat aatgccatcc gccagtgcct
     3661 caattttgcg cacccgcaac gactccagcg gatcgcgcgg caacatcgcc ggagcaacat
     3721 tcattaattc aatatattca gcgatgatcg gcgaatcaaa ccagcattcg ccctcttcgg
     3781 tcaccagcac cggcactttc cctaacgggt taaattgcgc cacgccattg tccgcgttat
     3841 agggcagttc atttataaat tcgaaagtaa tgcccttttc taacaacaga atagaaagtt
     3901 tgcgtacaaa cgggctggtg tagctaccga cgagtttcat gccgagtcct ttgtgcgagg
     3961 aaaaatatca gtatggctca ggctcgcgca aaacacaggt gatacaagtc tcaatgatcc
     4021 aaataaagat aatgcatcca actggtcatt cttagcagca ctttccgcat caccgaaaca
     4081 tggctgaaat ggtcggagta aactgccacc tgggcgtaga tgccgtcggg taaggcatcg
     4141 atatcagcgt tagggtccag ttcaatggtt cccagcacac cgtccgtgcc gggcacgacc
     4201 gttaatgatt gcaatacccc ctgcgcctga taagaaccgc ctggcacgac aggtaaaatg
     4261 ctggtcagtt tgccgtggaa cacctgccca ggtagcgcgt taaacaccac ttccgcatca
     4321 tcgccgggtt tcagacgtaa cagcgagttt tgccgaaatt gggcgacaat ttgccgtttt
     4381 tgctcgggga tgaagaccat caccggacgc agcggcaagg cagctgcgta tgtacctggg
     4441 cggatcagta cctgagtgac gtagccattg ctcggtgcgc gaatgacggt ctgctcaagg
     4501 ttatattttg cttcagtaag ttgcgctctt aagctcacaa tctgcgattg ctcgccgtta
     4561 accatactgt cgagctggct ctggatctgc gcctgctccg ccaccgagcc tttcaccagc
     4621 gcatcctgcg cgaggaaatt ttgccgcgca tcgtcgatgt cacgttccga gaacggattc
     4681 accgccgcct ggctgccttt caggtaacgt tgataatttt taaacagacg gtcgcgctcc
     4741 gctgaaacct gggtggtgtt ggcctgcgct tcagtgagct gcgcgcgcag cgtctttata
     4801 ttatgcgtcg ccgtcatcag gtctgcctga agcctgtcaa ctcgcgcctg gtaacgaacc
     4861 gggtcgagct taaaaagcac ctcgcccttc tgaataagct gattattttt gtcggtgact
     4921 tcagtaacaa ttcccgtcac ctgtggcgtg atggggatcg ctatcactgc cttttgcgcg
     4981 gtaaaagtgt aagggtggtt gtagttcatc aacaaaatca aaccactcac cagaaacacg
     5041 cctcccagcg ccgccgtcgc cagcgtccac tgattcaccg gaatgcggaa gattttaaag
     5101 accgcccacg ccagcgccac gtaagttaaa acaatcaata gatccattat tagatctccg
     5161 ggaacgtgga atagtcagtc tttttctcgg cggcgagttg gtgctccagc cgggaaatcc
     5221 ggtcagaaag tgcggcgatt tccgcgtcag tcgttttctc ttgcggcgta acatgcgact
     5281 gcatgcccca gccgcgctcc ggttgataga gcgttgccca gatccacagg aacggccaga
     5341 taacatgcag agtaaacagg ctcacccagc ccgccgtatg aatagcgtcg gcatggggat
     5401 ggttgcgctt tttggcaatc agataaggga tgtcatgtat cgcgatgatt ccataaaaaa
     5461 tcaccagaaa gacgaagatc agcactccca acgcgaaata gtttagaaac atatctgcct
     5521 cggattcacg tttatcagtg ttgttttttg gctggcagcc agaagggagt caggctgata
     5581 ttttgacaat aatccgggtt cgcgattctc gccataacac caaagaataa ttttaagagg
     5641 tgatgagttg cttagttaca taacgatttc atgacgaagg cataacatgc tgtagatcac
     5701 atcaggtgaa cgccgtaaga aaatatcttg tgattcagat cacaaagatt caacaaacca
     5761 tcaaaataaa aatgtgacat aactcacatt taaatgccat ttttagcgaa aatcgccgcc
     5821 ttgttgcttt tttacacaag cattttgtga tgaacatcac gtcaattacc tctctacccc
     5881 ctacatttat gtgactgata tcacacaaaa ggccgccgac tggacagtta accgattcag
     5941 tgccagattt cgcagtatct acaaggtccg gctacctctg ccgccacatt aacaaaaaac
     6001 ctcgggcttc cagcctgcgc gacagcaaac ataagaaggg gtgtttttat gtcatccgat
     6061 attaagatca aagtgcaaag ctttggtcgt ttcctcagca acatggtgat gccaaatatc
     6121 ggcgcgttta tcgcgtgggg tatcatcacc gcgttattta ttccaacagg gtggttaccg
     6181 aacgaaacgc tggcgaagct ggtcgggccg atgatcactt atctcctgcc gctgctgatc
     6241 ggttataccg gtggtaagct ggttggcggc gaacgtggcg gcgtagtcgg tgccatcacc
     6301 accatgggcg ttatcgtcgg cgcagatatg ccgatgttcc tcggctctat gattgcaggt
     6361 ccgctgggcg gctggtgcat taagcacttc gaccgctggg tagacggtaa gatcaaatcc
     6421 ggttttgaaa tgctggtgaa taacttctcc gcaggcatca tcgggatgat cctcgctatt
     6481 ctggcatttc tcggcattgg cccggttgtt gaagccctgt ctaaaatgct ggctgcgggc
     6541 gttaacttca tggttgtcca tgacatgctg ccgctggcgt ctatctttgt tgaaccggcg
     6601 aaaatcctgt tcctcaacaa cgccattaac cacggtatct tctcgccgct gggtattcag
     6661 cagtcccatg aactgggtaa atccatcttc ttcctgattg aagctaaccc aggtccgggt
     6721 atgggcgtgc tgctggcgta catgttcttt ggtcgtggta gcgctaaaca gtctgcgggc
     6781 ggtgcggcaa tcatccactt cctggggggt atccacgaaa tctacttccc gtatgtgctg
     6841 atgaatccgc gtctgattct ggccgttatc ctcggcggta tgactggcgt gttcacgctg
     6901 actatcctgg gcggtggtct ggtttctccg gcatctccgg gttctatcct tgctgtactg
     6961 gcgatgacgc caaaaggtgc ttacttcgct aacatcgcgg gtgtgtgtgc ggcgatggca
     7021 gtctccttcg ttgtctctgc cattctgctg aaaaccagca aagtgaaaga agaagatgat
     7081 atcgaagctg ctgcacgtcg cgttcaggaa atgaaagctg agtctaaagg cgcatctccg
     7141 ctgtctgcgg gcgatgtgac taacgacctg agccacgtcc gtaaaatcat cgttgcctgt
     7201 gatgccggta tgggttccag tgcgatgggt gcaggcgtgc tgcgtaagaa aattcaggat
     7261 gcaggtctgt cgcagatttc ggtcactaac agcgcgatca acaacctgcc gccagatgtg
     7321 gacctggtca tcactcaccg tgacctgacc gaacgcgcta tgcgccaggt tccgcaggca
     7381 cagcatattt cgctgaccaa cttcctcgac agcggcctgt acaccagcct gaccgaacgt
     7441 ctggttgctg cccagcgcca tactgaaaac gaagtgaaag tgaaagacag tctgaaagac
     7501 agctttgacg attccagtgc taatctgttc aaactgggcg cggagaacat cttcctcggt
     7561 cgcaaagcgg ccaccaaaga agaagcgatt cgttttgctg gcgagcagct ggtgaaaggc
     7621 ggttacgttg agccggaata cgttcaggcg atgctggatc gtgaaaaact gaccccgact
     7681 tatctgggtg agtctatcgc ggtgccacac ggtacggttg aagcgaaaga tcgcgtactg
     7741 aaaacgggcg tggtgttctg ccagtacccg gaaggcgtgc gcttcggtga agaagaagat
     7801 gacattgccc gtctggtgat tggtattgct gcccgtaaca acgagcacat tcaggtgatc
     7861 accagcctga ccaatgcact ggatgatgag tccgtcatcg agcgtctggc acacaccacc
     7921 agcgtggatg aagtgctgga attgctggca ggtcgtaagt aatccattcc caccctctcc
     7981 gcatgaagaa ggtggggcta attgcctgat gcgcttcgct tatcaggcct acaacataaa
     8041 gcacaatttg ttttgtcggt cggataaggt gtttacgccg catccggcag tcggtgcaca
     8101 atgcctgatg cgacgctgac gcgtcttatc aggcctacag gtcagtgcca aacacgtagg
     8161 ccggataagg cgtttacgcc gcatccggca aaaagcccct ctcctcacgg agagggtttg
     8221 ggtgagggaa aagcctcacc ccagccctct cgggtaaaaa cattgatgaa ggttaatact
     8281 atgaaagcat tacattttgg cgcaggtaat atcggtcgtg gctttatcgg taaactgctg
     8341 gcggacgcgg gtatccaact gacgtttgcc gatgtaaatc aggtggtgct tgatgccctg
     8401 aatgcccgtc atagctatca ggttcatgtg gttggtgaaa ccgagcaggt agataccgtt
     8461 tctggcgtcg atgccgtcag cagcattggc gatgatgtcg ttgatctgat tgctcaggtc
     8521 gatgtagtca ctaccgccgt tggccctgtc gtactggaac gtattgctcc ggccatcgcc
     8581 aaagggctgg tgaaacgtaa agaacaaggt aatgaatccc cactgaacat catcgcctgt
     8641 gaaaacatgg tccgcggcac cacgcagctg aaaggccatg tgatgaacgc cctgccggaa
     8701 gatgccaaag cgtgggtaga agaacacgtt ggctttgtcg attccgccgt tgaccgcatc
     8761 gtaccgcctt cggcttcggc aactaacgat ccgctggaag tgacagtaga aaccttcagc
     8821 gaatggattg tcgataaaac gcagttcaaa ggcacgctgc caaacatccc tggcatggaa
     8881 ttaaccgaca atctgatggc atttgtcgaa cgtaaactct tcaccctgaa tacaggtcat
     8941 gctataaccg cgtacctcgg aaaactggcc ggtcatcaga ccattcgtga cgcaattctc
     9001 gacgagaaaa tccgcgcggt ggtaaaaggg gcaatggaag aaagcggcac ggtactgatc
     9061 aagcgctacg gctttgatgc tgacaaacat gcggcataca tccagaaaat cctcggtcgt
     9121 ttcgagaacc cgtacctgaa agatgatgta gaacgcgtag gccgtcagcc gctgcgtaaa
     9181 ctgagtgctg gcgaccgtct gatcaagcca ctgctcggaa ccctggaata tggtctgcca
     9241 cataaaaacc tgattgaagg tattgccgct gcaatgcact tccgcagtga agatgacccg
     9301 caggctcagg aactggcagc gctgattgcc gacaaaggcc cacaagccgc gctggcagag
     9361 atttccggtc ttgatgccaa cagcgaggtt gtatccgagg cggtaaccgc ttataaagca
     9421 atgcaataat ggtggaccag gcgcaggata ccctgcgccc gaataacaga ttgtcagata
     9481 tgcaggcaac aatggaacaa acccaggcct ttgaaaaccg tgtgcttgag cgtctgaatg
     9541 ctggcaaaac cgtgcgaagc tttctgatca ccgccgtcga gcttttgacc gaggcggtaa
     9601 atcttctggt gcttcaggta ttccgcaaag acgattacgc ggtgaagtat gctgtagaac
     9661 cgttactcga cggcgatggt ccgctgggcg atctttctgt gcgtttaaaa ctcatttacg
     9721 ggttgggcgt cattaaccgc caggaatacg aagatgcgga actgctgatg gcattgcgtg
     9781 aagagctaaa tcacgacggc aacgagtacg cttttaccga cgacgaaata cttggaccct
     9841 ttggtgaact gcattgcgtg gcggcgttac caccgccgcc acagtttgaa cctgcagact
     9901 ccagtttgta tgcaatgcaa attcagcgtt accaacaggc tgtgcgatca acaatggtcc
     9961 tttcactgac agagctgatt tccaaaatca gcttaaaaaa agcctttcaa aagtaagtac
    10021 tttcgcttac ttctcctcta cctgtttcgg acgataaagc tgacgataat actccagacg
    10081 ttgtagatat aacggcacgc tttcatcagg tattccggac ggaatcgcgt tacggggagg
    10141 tagttttttc agatactccc ggaacgcctg gctggatgcc atgaaatcta cggctttatc
    10201 gataagaagc ggaacgtttt cacctaattt gcccatgatg atatctccgt attaccccgc
    10261 gccgggaatg cgcggccgcc aattttagtt tagatcccgg tatcaatgac attaaggaaa
    10321 acgtgctcaa tcctcaataa tcacctgctt ctgttgacta tcttttttct ttaaatttct
    10381 taactaatca atgtattggg tgatttttta acgcataaat ggttacaatc tggaactatt
    10441 acaatgccat cagttgtgaa caaagcacct ggtcgcgcat actagaggct ataaatttat
    10501 ctctatcaga agtcatcaca tgaaagaagt cgaaaaaaac gaaatcaaac gtctcagcga
    10561 tcgtctggac gccatccgtc accagcaggc cgatctatcg ctggttgaag ccgcagacaa
    10621 atatgccgaa ctggaaaaag agaaagccac tctggaagca gaaattgctc gcctgcgcga
    10681 agttcacagc cagaaactca gcaaagaagc gcagaaactg atgaagatgc cgttccagcg
    10741 tgcaatcacc aaaaaagagc aggctgatat gggcaaactg aaaaaaagtg ttcgcggact
    10801 ggtggttgtg cacccaatga ccgcactggg ccgcgaaatg ggcctgcaag agatgacagg
    10861 gttctcaaag actgcgtttt aagttccttt ctcgtcgctg taatcacagc ctctcaccat
    10921 ttactcgctc ctgctggcac atgcaggagc ctgtttcaga gatcgaatct ttataacgtt
    10981 cttaacatta tatattcaac tccacctaca cttctgagcg atcgtagcag gctaaattct
    11041 gaaaatcagg tacacattaa agaatgatgt atcaatatca acaatgaatt gccgcatatc
    11101 aatgctcgaa acataattaa ttatttaaat ttttcaatca gcttcgcaaa ttccacaata
    11161 aaacatcact caactgaata tttatctttc cctgccctgt gcctttatcc ggtgaaacat
    11221 ttgacatcga ttgacaccga tcattaaaat ccgcgcgatt aatattattg gctacaaact
    11281 gaaatcgttt gacatttaat aaaatattcg cacaaccaat taatacaaaa actctctaac
    11341 aaatttattt tgcggatatg ccccactcca atcattcata aaaaaaatga ttgtgccgta
    11401 attatcaact gtatcgggat tatgaaaagg aattcatcct atgaacctaa agaaaacact
    11461 gttaagcgtg ttaatgatat tgcaactttg cttattggta gggtgtgact atattgaaaa
    11521 agcgagtaag gtcgacgatc tcgttacaca gcaagagtta caaaaaagca aaattgaggc
    11581 gcttgaaaaa caacaagaac tcgacaagcg caagatagaa cactttgaaa aacaacaaac
    11641 taccatcata aacagtacca aaacgctcgc tggcgtggtg aaggcagtta aagacaaaca
    11701 ggacgaattt gtctttacag aatttaaccc ggcacaaacc caatacttca ttttaaataa
    11761 cggttctgtt ggtttggcag ggaaaatact gtctattgac gcagtagaaa acggcagcgt
    11821 tattcgtatt tcactggtta acttattaag tgttcccgta tcaaatatgg gtttctacgc
    11881 gacatggggg ggagaaaaac ccaccgacat caacgcatta gcaaaatggc agcaattgct
    11941 atttagtacc tcaatgaact cctccctgaa attattacca ggtcaatggc aagacattaa
    12001 tttgacgcta aaaggtgtct cgcccaacaa cctcaaatat ctgaaattag ccatcaacat
    12061 ggcaaatatt cagttcgacc gtcttcaacc tgctgaatct ccacagcgga aaaacaaaaa
    12121 ataaatgcag cttctgcgct tatatcaagg aatagagagc atcaataatg aacaaaatat
    12181 ttaaagttat ctggaatccg gcgacaggca gttacagcgt cgccagcgaa acggcgaaga
    12241 gccgtggtaa aaaatcaggg cgcagtaagc tgttaatttc tgcattaatt gtcaccagtg
    12301 caatttttac tccaggagca tacgcaggac tctctctcga cggcggtgac attttcgaat
    12361 cgactccatt gaccaattac tggcttgcta ttggtcaagg gtctgtagca accaccaata
    12421 atgggggaac tgacggagtt gctatggcaa taggattgaa agccaaagca ctcggggcgg
    12481 gaagtacagc atttggctat gatgctgaag caagcggaga tcgagcaata gcgttcggac
    12541 aattaacaga agcatcaggc aatagaacaa tagcaatggg tagtggagca actgccactg
    12601 gtgaccattc tctggcgcta gggggggcaa ctaagactct aggtatgtat tccgtggcaa
    12661 taggccggga tgctactaca gatagcgact acgcgctttc tatggggcat atggcgaagg
    12721 cgaatggact atacagcctg gcaatgggtg caggaagtgc tacttcgaac gataacgcca
    12781 tcgcgattgg gaaaaaaacg caagcccagg gagtgaattc tatagccctg ggtaatgcaa
    12841 gccaggcatc gggctactcc agccttgcaa taggtgagtt aagtgagacc ggtgctgaaa
    12901 atgctattgc actgggaaaa ctcagtaacg caagtaaaat caattcaatc gccctgggta
    12961 gtaacagcac tgcatctggt gagggctccg ttgcgctggg agagaatagt tttgctggag
    13021 gcataaacag tcttgctttg ggtagtcaat ccaatgcaaa cggtgataac gcagttgccc
    13081 tcggtgtagg atctgtcgca gcgcaagaca ataccgtttc ggtagggaac agcacaacac
    13141 aacgcaaaat taccaatatg gctgccggtc aaataagaaa tggcagtact gaggccatta
    13201 acggttcaca actttatgga ctaagtgact cagtcgcagc ccgactcggg ggtggtgctg
    13261 gcgtaaatga agatggaagt ataaatgcgc caagctacaa gctgaaaagc aacatctaca
    13321 ataatgtggg tgatgcgctt ttaggaatcg acaatgacac tctgcactgg gacaaaacca
    13381 ataaagcatt tagtgccagc tatctcgcca aaaacgctga tggctcccta aaggagcgct
    13441 cagaccaaaa caaaatcatt aatgttgcaa agggtactat ttccgccacc agcaccgatg
    13501 tagtaaacgg ctctcaattg tacgacctgc agcaggatgc cctgttgtgg aacggcactg
    13561 cattcagcgc cgcacacggt agtgatacca ccagcaaaat cactaatgtt gctaaaggta
    13621 cggtttctgc aaccagcacc gatgtagtaa acggctctca attgtacgac ctgcagcagg
    13681 atgcgctgtt gtggaacggc acagcattca gtgccgcaca cggcaccgaa gccaccagca
    13741 aaatcactaa tgttgaagat ggtactattt cagacaccag taaagatgcg gttaacggtt
    13801 cccagttgaa agaaaccaaa gacgatgtgg ccaccaacac agccaatatc gccgataaca
    13861 ctgctgatat tgcaaccaac actaccaata tcgccgataa cactgctgat
//
LOCUS       DADSYM010000125        13814 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2415
            SAMN08039579-rid11723173.denovo.126, whole genome shotgun sequence.
ACCESSION   DADSYM010000125 DADSYM010000000
VERSION     DADSYM010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039579
            Sequence Read Archive: SRR6362533
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13814)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13814)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980162.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 03:39:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,484
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 4,347
            CDSs (with protein)               :: 4,347
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 43 of 85
            Pseudo Genes (incomplete)         :: 37 of 85
            Pseudo Genes (internal stop)      :: 19 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13814
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039579-rid11723173.denovo.126"
                     /strain="LGE2415"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8302 N 95.9628 W"
                     /collection_date="2015-08-24"
     gene            complement(102..854)
                     /gene="yecC"
                     /locus_tag="J1Y83_003335"
     CDS             complement(102..854)
                     /gene="yecC"
                     /locus_tag="J1Y83_003335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine ABC transporter ATP-binding protein
                     YecC"
                     /protein_id="HBA7631452.1"
                     /translation="MSAIEVKNLVKKFHGQTVLHGIDLEVKPGEVVAIIGPSGSGKTT
                     LLRSINLLEQPEAGTITVGDITIDTARSLSQQKSLIRQLRQHVGFVFQNFNLFPHRTV
                     LENIIEGPVIVKGEPKEEATARARELLAKVGLAGKETSYPRRLSGGQQQRVAIARALA
                     MRPEVILFDEPTSALDPELVGEVLNTIRQLAQEKRTMVIVTHEMSFARDVADRAIFMD
                     QGRIVEQGVAKALFADPQQPRTRQFLEKFLLQ"
     gene            complement(851..1519)
                     /gene="tcyL"
                     /locus_tag="J1Y83_003336"
     CDS             complement(851..1519)
                     /gene="tcyL"
                     /locus_tag="J1Y83_003336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine ABC transporter permease"
                     /protein_id="HBA7631453.1"
                     /translation="MQESIQLVIDSLPFLLKGAGYTLQLSIGGMFFGLLLGFILALMR
                     LSPIWPVRWLARFYISIFRGTPLIAQLFMIYYGLPQFGIELDPIPSAMIGLSLNTAAY
                     AAETLRAAISSIDKGQWEAAASIGMTPWQTMRRAILPQAARVALPPLSNSFISLVKDT
                     SLAATIQVPELFRQAQLITSRTLEVFTMYLAASLIYWIMATVLSTLQNHFENQLNRQE
                     REPK"
     gene            complement(1534..2520)
                     /gene="dcyD"
                     /locus_tag="J1Y83_003337"
     CDS             complement(1534..2520)
                     /gene="dcyD"
                     /locus_tag="J1Y83_003337"
                     /EC_number="4.4.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-cysteine desulfhydrase"
                     /protein_id="HBA7631454.1"
                     /translation="MPLHNLTRFPRLEFIGAPTPLEYLPRFSDYLGREIFIKRDDVTP
                     MAMGGNKLRKLEFLAADALREGADTLITAGAIQSNHVRQTAAVAAKLGLHCVALLENP
                     IGTTAENYLTNGNRLLLDLFNTQIEMCDALTDPNAQLEELATRVEAQGFRPYVIPVGG
                     SNALGALGYVESALEIAQQCEGAVNISSVVVASGSAGTHAGLAVGLEQLMPESELIGV
                     TVSRSVADQLPKVVNLQQAIAKELELTASAEILLWDDYFAPGYGVPNDEGMEAVKLLA
                     RLEGILLDPVYTGKAMAGLIDGISQKRFKDEGPILFIHTGGAPALFAYHPHV"
     gene            complement(2625..3425)
                     /gene="tcyJ"
                     /locus_tag="J1Y83_003338"
     CDS             complement(2625..3425)
                     /gene="tcyJ"
                     /locus_tag="J1Y83_003338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine ABC transporter substrate-binding
                     protein"
                     /protein_id="HBA7631455.1"
                     /translation="MKLAHLGRQALMGVMAVALVAGMSVKSFAEEGLLNKVKERGTLL
                     VGLEGTYPPFSFQGDDGKLTGFEVEFAQQLAKHLGVEASLKPTKWDGMLASLDSKRID
                     VVINQVTISDERKKKYDFSTPYTISGIQALVKKGNEGTIKTADDLKGKKVGVGLGTNY
                     EEWLRQNVQGVDVRTYDDDPTKYQDLRVGRIDAILVDRLAALDLVKKTNDTLAVTGEA
                     FSRQESGVALRKGNEDLLKAVNDAIAEMQKDGTLQALSEKWFGADVTK"
     gene            complement(3513..4064)
                     /gene="fliZ"
                     /locus_tag="J1Y83_003339"
     CDS             complement(3513..4064)
                     /gene="fliZ"
                     /locus_tag="J1Y83_003339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310687.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulatory protein FliZ"
                     /protein_id="HBA7631456.1"
                     /translation="MMVQHLKRRPLSRYLKDFKHSQTHCAHCRKLLDRITLVRDGKIV
                     NKIEISRLDTLLDENGWQVEQQSWAALCRFCGDLHCKTQSDFFDIIGFKQFLFEQTEM
                     SPGTVREYVVRLRRLGNHLHEQNISLDQLQDGFLDEILAPWLPSTSTNNYRIALRKYQ
                     HYQRQTCTGLVQKSSSQPASDIY"
     gene            complement(4110..4829)
                     /locus_tag="J1Y83_003340"
     CDS             complement(4110..4829)
                     /locus_tag="J1Y83_003340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor FliA"
                     /protein_id="HBA7631457.1"
                     /translation="MNSLYTAEGVMDKHSLWQRYVPLVRHEALRLQVRLPASVELDDL
                     LQAGGIGLLNAVERYDALQGTAFTTYAVQRIRGAMLDELRSRDWVPRSVRRNAREVAQ
                     AIGQLEQELGRNATETEVAERLGIDIADYRQMLLDTNNSQLFSYDEWREEHGDSIELV
                     TDDHQRENPLQQLLDSNLRQRVMEAIETLPEREKLVLTLYYQEELNLKEIGAVLEVGE
                     SRVSQLHSQAIKRLRTKLGKL"
     gene            complement(5149..6795)
                     /locus_tag="J1Y83_003341"
     CDS             complement(5149..6795)
                     /locus_tag="J1Y83_003341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000079739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliC/FljB family flagellin"
                     /protein_id="HBA7631458.1"
                     /translation="MAQVINTNSLSLITQNNINKNQSALSSSIERLSSGLRINSAKDD
                     AAGQAIANRFTSNIKGLTQAARNANDGISVAQTTEGALSEINNNLQRIRELTVQASTG
                     TNSDSDLDSIQDEIKSRLDEIDRVSGQTQFNGVNVLAKDGSMKIQVGANDGQTITIDL
                     KKIDSDTLGLNGFNVNGKGETANTAATLKDMSGFTAAAAPGGTVGVTQYTDKSAVASS
                     VDILNAVAGADGNKVTTSADVGFGTPAAAVTYTYNKDTNSYSAASDDISSANLAAFLN
                     PQAGDTTKATVTIGGKDQDVNIDKSGNLTAADDGAVLYMDATGNLTKNNAGGDTQATL
                     AKLATATGAKAATIQTDKGTFTSDGTAFDGASMSIDANTFANAVKNDTYTATVGAKTY
                     SVTTGSAAADTAYMSNGVLSDTPPTYYAQADGSITTTEDAAAGKLVYKGSDGKLTTDT
                     TSKAESTSDPLAALDDAISQIDKFRSSLGAVQNRLDSAVTNLNNTTTNLSEAQSRIQD
                     ADYATEVSNMSKAQIIQQAGNSVLAKANQVPQQVLSLLQG"
     gene            7061..8467
                     /gene="fliD"
                     /locus_tag="J1Y83_003342"
     CDS             7061..8467
                     /gene="fliD"
                     /locus_tag="J1Y83_003342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar filament capping protein FliD"
                     /protein_id="HBA7631459.1"
                     /translation="MASISSLGVGSGLDLSSILDSLTAAQKATLTPISNQQSSFTAKL
                     SAYGTLKSALTTFQTANTALSKADLFSATSTTSSTTAFSATTAGNAIAGKYTISVTHL
                     AQAQTLTTRTTRDDTKTAIATSDSKLTIQQGGDKDPISIDISAANSSLSGIRDAINNA
                     KAGVSASIINVGNGEYRLSVTSNDTGLDNAMTLSVSGDDALQSFMGYDASASSNGMEV
                     SVAAQNAQLTVNNVAIENSSNTISDALENITLNLNDVTTGNQTLTITQDTSKAQTAIK
                     DWVNAYNSLIDTFSSLTKYTAVDAGADSQNSSNGALLGDSTLRTIQTQLKSMLSNTVS
                     SSNYKTLAQIGITTDPSDGKLELDADKLTAALKKDASGVGALIVGDGKKTGITTTIGS
                     NLTSWLSTTGIIKAATDGVSKTLNKLTKDYNAASDRIDAQVARYKEQFTQLDVLMTSL
                     NSTSSYLTQQFENNSNSK"
     gene            8492..8902
                     /gene="fliS"
                     /locus_tag="J1Y83_003343"
     CDS             8492..8902
                     /gene="fliS"
                     /locus_tag="J1Y83_003343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FliS"
                     /protein_id="HBA7631460.1"
                     /translation="MYAAKGTQAYAQIGVESAVMSASQQQLVTMLFDGVLSALVRARL
                     FMQDNNQPGKGVSLSKAINIIENGLRVSLDEESKDELTQNLIALYSYMVRRLLQANLR
                     NDVSAVEEVEALMRNIADAWKESLLSPSLIQDPV"
     gene            8902..9267
                     /gene="fliT"
                     /locus_tag="J1Y83_003344"
     CDS             8902..9267
                     /gene="fliT"
                     /locus_tag="J1Y83_003344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulatory protein FliT"
                     /protein_id="HBA7631461.1"
                     /translation="MNNAPHLYFAWQQLVEKSQLMLRLATEEQWDELIASEMAYVNAV
                     QEIAHLTEEVAPSTTMQEQLRPMLHLILDNESKVKQLLQIRMDELAKLVGQSSVQKSV
                     LSAYGDQGGFVLAPQDNLF"
     gene            9345..10832
                     /gene="amyA"
                     /locus_tag="J1Y83_003345"
     CDS             9345..10832
                     /gene="amyA"
                     /locus_tag="J1Y83_003345"
                     /EC_number="3.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HBA7631462.1"
                     /translation="MRNPTLLQCFHWYYPEGGKLWPELAERADGFNDIGINMVWLPPA
                     YKGASGGYSVGYDSYDLFDLGEFDQKGSIPTKYGDKAQLLAAIDALKRNDIAVLLDVV
                     VNHKMGADEKEAIRVQRVNADDRTQIDEEIIECEGWTRYTFPARAGQYSQFIWDFKCF
                     SGIDHIENPDEDGIFKIVNDYTGEGWNDQVDDELGNFDYLMGENIDFRNHAVTEEIKY
                     WARWVMEQTQCDGFRLDAVKHIPAWFYKEWIEHVQEVAPKPLFIVAEYWSHEVDKLQT
                     YIDQVEGKTMLFDAPLQMKFHEASRMGRDYDMTQIFTGTLVEADPFHAVTLVANHDTQ
                     PLQALEAPVEPWFKPLAYALILLRENGVPSVFYPDLYGAHYEDVGGDGQTYPIDMPII
                     EQLDELILARQRFAHGVQTLFFDHPNCIAFSRSGTDEYPGCVVVMSNGDDGEKTIHLG
                     ENYGNKTWRDFLGNRQESVVTDENGEATFFCNGGSVSVWVIEEVI"
     gene            complement(10866..11279)
                     /locus_tag="J1Y83_003346"
     CDS             complement(10866..11279)
                     /locus_tag="J1Y83_003346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310694.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HBA7631463.1"
                     /translation="MKKLAIAGALMLLAGCAEVENYNNVVKTPAPDWLTGYWQTKGPQ
                     RALVSPEAIGSLIVTKEGDTLDCRQWQRVIAVPGKLTLMSDDLTNVTVKRELYEVERD
                     GNTIEYDGMTMERVDRPTAECAAALDKAPLPTPLP"
     gene            11466..12671
                     /gene="yedE"
                     /locus_tag="J1Y83_003347"
     CDS             11466..12671
                     /gene="yedE"
                     /locus_tag="J1Y83_003347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium metabolism membrane protein YedE/FdhT"
                     /protein_id="HBA7631464.1"
                     /translation="MSWQQFKHAWLIKFWAPIPAVIAAGILSTYYFGITGTFWAVTGE
                     FTRWGGQLLQLFGVHAEEWGYFKIIHLEGSPLTRIDGMMILGMFGGCFAAALWANNVK
                     LRMPRSRIRIMQAIIGGIIAGFGARLAMGCNLAAFFTGIPQFSLHAWFFAIATAIGSW
                     FGARFTLLPIFRIPVKMQKVSAASPLTQKPDQARRRFRLGMLVFFGMLGWALLTAMNQ
                     PKLGLAMLFGVGFGLLIERAQICFTSAFRDMWITGRTHMAKAIIIGMAVSAIGIFSYV
                     QLGVEPKIMWAGPNAVIGGLLFGFGIVLAGGCETGWMYRAVEGQVHYWWVGLGNVIGS
                     TILAYYWDDFAPALATDWDKINLLKTFGPMGGLLVTYLLLFAALMLIIGWEKRFFRRA
                     APQIAKEIA"
     gene            12668..12901
                     /gene="yedF"
                     /locus_tag="J1Y83_003348"
     CDS             12668..12901
                     /gene="yedF"
                     /locus_tag="J1Y83_003348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase-like selenium metabolism
                     protein YedF"
                     /protein_id="HBA7631465.1"
                     /translation="MKNIVPDYRLDMVGEPCPYPAVATLEAMPQLKKGEILEVVSDCP
                     QSINNIPLDARNHGYTVLDIQQDGPTIRYLIQK"
     gene            13010..13678
                     /locus_tag="J1Y83_003349"
     CDS             13010..13678
                     /locus_tag="J1Y83_003349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
                     /protein_id="HBA7631466.1"
                     /translation="MCGRFAQSQTREDYLALLAEDIERDIPYDPEPIGRYNVAPGTKV
                     LLLSERDEHLHLDPVFWGYAPGWWDKPPLINARVETAATSRMFKPLWQHGRAICFADG
                     WFEWKKEGDKKQPYFIYRADGQPVFIAAIGSTPFERGDEAEGFLIVTAAADQGLVDIH
                     DRRPLVLSPEAAREWMRQEISGKEASEIAASGCVPANQFTWHPVSCAVGNVKNQGAEL
                     IQPV"
BASE COUNT         3370 a         3574 c         3421 g         3449 t
ORIGIN      
        1 ttatgttatt aatgagttat gctgatttgt taagcagttt tatcacgttt gaaatggcgt
       61 cccgccccga ctggtgaatc gtcggggctg attttttctt attattgcag cagaaacttc
      121 tccaggaact ggcgggtgcg aggctgctga ggatcagcaa ataacgcttt tgctaccccc
      181 tgctcgacta tccgcccttg gtccataaaa atcgcccggt ccgcaacatc ccgggcaaag
      241 ctcatttcgt gcgtcacaat caccatcgtg cgcttttcct gcgccagttg acgaatggtg
      301 ttcaggactt cgcccaccag ctctggatcc agcgctgacg ttggctcatc aaacaaaatc
      361 acttcaggac gcattgccag agcacgcgca atcgcaacgc gctgctgttg accgccagac
      421 aaacgacgtg gataactggt ttctttaccg gccagcccaa ctttcgccag cagctcgcga
      481 gcgcgcgccg tggcctcttc tttcggttcg cctttgacga tcaccggccc ttcaataatg
      541 ttttccagca ccgtacgatg cggaaacaga ttaaagttct ggaagacaaa cccgacgtgc
      601 tgacgcaact ggcgaatcag cgatttttgc tgacttaatg aacgtgctgt atcaatagtg
      661 atatcgccaa cggtgatggt tcccgcttcc ggttgttcca gcagatttat gctgcgtagc
      721 aacgtggttt tgccggaacc actcggaccg ataatagcca ctacttcgcc aggcttcacc
      781 tcaaggtcga taccatgcag caccgtctga ccgtggaatt ttttcaccag gttcttaact
      841 tcaatggcac tcatttgggt tccctctcct ggcgattaag ttgattctca aaatggttct
      901 gcaacgtcga taacactgtc gccatgatcc agtagatgag cgaagccgcc aggtacatgg
      961 tgaacacctc cagcgtgcgc gaggtaatca actgcgcctg acggaacagc tccggcacct
     1021 ggattgtcgc ggccagcgac gtatctttta ccaggctgat aaagctgttc gacagcggcg
     1081 gcaacgccac ccgcgccgcc tgtggcaaaa tcgcgcgacg catggtctgc catggcgtca
     1141 taccaatact ggctgcagct tcccactgac ctttatcaat agaagaaatg gcggcgcgca
     1201 gcgtttcagc agcataggcg gcagtgttca gcgacagacc aatcattgct gacggaatag
     1261 gatctaattc gataccaaac tgtggcagtc cgtaatagat cataaacagt tgtgcgatga
     1321 gcggcgtacc acgaaaaata gagatataaa aacgcgccag ccagcgcacg ggccagattg
     1381 gcgacaggcg catcagcgcg agaataaacc cgagcagtaa gccaaaaaac atgccgccga
     1441 tactgagttg cagcgtatac ccagcccctt tcagcaggaa cggcaaggaa tcaataacca
     1501 gttgtatact ttcttgcatg agcgttcccg gagctaaacg tggggatgat aggcgaacag
     1561 cgcaggcgca ccgccggtat gaataaacaa aatcggccct tcatctttga agcgtttctg
     1621 actgataccg tcaatcagcc ccgccatcgc ttttccggta tacacaggat caagcagaat
     1681 gccttccagc cgcgccagca atttcactgc ttccatgcct tcgtcgttcg gcacgccgta
     1741 gccaggtgca aaatagtcat cccagagtaa aatttccgct gatgcggtca gctccagttc
     1801 tttcgcaatc gcctgttgga ggttaaccac tttcggcaat tgatcggcaa cagaacgcga
     1861 cacagtcacg ccaatcagtt cgctttcagg catcagctgt tccagcccaa cagccagtcc
     1921 ggcgtgagtt ccggcactgc ccgatgcgac caccaccgac gaaatattaa ccgccccttc
     1981 acactgttgc gcgatttcca gcgcactctc cacataaccc agcgcgccca aagcattaga
     2041 accgccaacc ggaatgacat acgggcgaaa gccttgtgct tcgactcgcg tcgccagctc
     2101 ttccagttgg gcattgggat cggtcagtgc gtcgcacatt tcaatctggg tattgaacag
     2161 atccagcaac aaacgattgc cgttggttaa atagttttcc gcggttgtac caataggatt
     2221 ttccagcagc gccacgcagt gcagaccgag tttcgcagcg actgcggcag tctggcgcac
     2281 atggttagac tggatcgccc cggcagtaat cagcgtatcg gcaccttcac gcagagcatc
     2341 tgccgcgaga aattccagct tacgtaattt attgccgccc attgccatgg gagtgacgtc
     2401 atcccgtttg atgaaaattt cccgtccaag ataatcagaa aagcgcggca gatattcgag
     2461 cggcgttggc gcgccgataa actccagccg tggaaaacgg gttaaattat gcagtggcat
     2521 aacagcctcc gatgtgtgtt gttgtgattt tcttattatg cacgctgaaa acgcgtaaat
     2581 aaaaaaggcg ctagtgaaag cgcccttttt tgtcattatg ctgattattt ggtcacatca
     2641 gcaccaaacc atttttcgga aagggcttgc aaagtgccat ctttttgcat ttccgcaatt
     2701 gcatcattca ccgctttcag caggtcttca tttcctttac gcagcgccac gccagactcc
     2761 tgacgggaga atgcttcacc ggttactgcc agcgtatcgt tggttttttt caccagatcc
     2821 agcgccgcca gacgatcaac gaggatcgca tcgatacggc ctacgcgcag atcctgatat
     2881 ttggtcgggt catcatcata ggtacgcaca tcgacgccct gaacattctg acgcagccac
     2941 tcttcatagt tggtgcccag accgacaccc acttttttgc ctttcagatc atcagccgtt
     3001 ttaatggtgc cttcgttgcc ttttttcacc agcgcctgaa taccagaaat ggtgtacggg
     3061 gtagagaaat cgtatttttt cttgcgctcg tcagaaatgg tgacctggtt aatcaccaca
     3121 tcaatgcgtt tagagtccag cgacgccagc ataccgtccc atttggtcgg ttttagtgac
     3181 gcttcaacgc caagatgttt tgccagctgt tgggcaaatt ccacttcaaa accggttaat
     3241 ttgccgtcat ccccctgaaa actgaacggc ggataagttc cttccagccc taccagcagc
     3301 gtgccgcgct ctttgacttt attaagcaga ccttcttctg caaaactttt aacgctcata
     3361 cccgcaacca gcgccacggc catcacaccc atcaatgcct gacgtcccag atgtgctaat
     3421 ttcatattta ccccgaatgt tgttatgtct gtttgcagtg tagagccatt gtttgtaaac
     3481 acaaaaacaa ctccgctaca tcttattctt atttaatata tatcagaagc aggttggctg
     3541 gaggatttct gcacaagtcc ggtacacgtt tgacgctgat agtgttgata cttccgtaac
     3601 gcgatgcggt aattgttggt gctggtggag ggcagccacg gggcgaggat ttcatcaagg
     3661 aaaccgtcct gcagctggtc gagggaaata ttttgctcgt gtagatgatt ccccaaacgg
     3721 cgcagacgaa cgacatattc acgcaccgta ccagggctca tttcagtttg ctcaaaaaga
     3781 aattgcttaa agccgataat atcgaaaaaa tcactctgcg ttttgcaatg taaatcaccg
     3841 caaaatcggc acaatgccgc ccatgattgt tgttccactt gccagccatt ttcatcaagc
     3901 agcgtgtcca ggcgggaaat ctcgatttta ttcactattt tgccgtcgcg aactaaggta
     3961 atgcgatcga gtaatttacg gcaatgcgcg caatgggtct ggctgtgttt aaagtcttta
     4021 agatagcggc ttaatggccg tcttttcagg tgctgcacca tcattaagaa ctcctggtaa
     4081 tcaaaattaa agtgcggcat ttactgacgt tataacttac ccagtttagt gcgtaaccgt
     4141 ttaatagcct ggctgtgtaa ctgactgacc cgcgattccc cgacctccag caccgcacca
     4201 atctctttga gattcagctc ttcctgataa tagagggtta ataccagttt ttcgcgctcc
     4261 ggcaacgttt cgatggcttc catcacccgc tggcgcagat tactgtccag cagttgttgt
     4321 agcgggtttt ctcgctgatg atcatcagta accagttcga tgctatcgcc gtgctcttcg
     4381 cgccactcat cgtaggagaa gagctggctg ttattggtgt cgagcaacat ttggcgataa
     4441 tcggcaatat cgatccctaa acgttccgct acctcagttt ccgtggcgtt gcggccaagt
     4501 tcctgctcca gttgccctat tgcctgtgcc acttcacgcg cgttgcgtcg cacgctgcgc
     4561 ggcacccagt cacggctgcg aagttcatcc agcatagcgc cacggatacg ctgcactgcg
     4621 taagttgtaa atgccgttcc ttgtagggcg tcatagcgtt cgacggcatt aagtaaccca
     4681 atgccgcccg cctgtagcag atcgtcaagt tccacgctcg cgggcagtcg aacctgcagg
     4741 cgcaatgctt cgtgacgcac cagcgggaca taacgctgcc acagcgagtg tttatccatt
     4801 acaccttcag cggtatagag tgaattcacg ataaacagcc ctgcgttata tgagttatcg
     4861 gcatgattat ccgtttctgc agggttttta atcggacgat tagtgggtga aatgaggggt
     4921 tatttggggg ttaccggtaa attgcgggca gaaaaaaccc cgccgttggc ggggaagcac
     4981 gttgctgaca aattaccatt catgttgccg gatgcggcgt aaacgcctta tccggcctac
     5041 aaaaatgtgc aaattcaata aattgcaatt ccccttgtag gcctgataag cgcagcgcat
     5101 caggcaattt ggcgttgccg tcagtctcag ttaatcaggt tacgacgatt aaccctgcag
     5161 cagagacaga acctgctgcg gtacctggtt ggcttttgcc agtacggagt taccggcctg
     5221 ctggataatc tgcgctttcg acatgttgga cacttcggtc gcatagtcgg cgtcctgaat
     5281 acgggactgc gcttcagaca ggttggtagt ggtgttgttc aggttggtca ctgcggaatc
     5341 cagacggttt tgcaccgcac ccagggagga gcggaatttg tcgatctggc tgatagcgtc
     5401 gtcaagagct gccagcggat ctgatgttga ttctgctttg ctagtcgtat ccgttgttaa
     5461 cttaccatcg gaacctttgt agaccagttt accggcagcc gcatcctcag tagttgtgat
     5521 acttccatca gcttgtgcat agtaagttgg cggagtatca ctgagaaccc cattgctcat
     5581 ataagcggtg tctgcagcag cagaacctgt tgttacgcta taagtcttag cacctacagt
     5641 ggcagtataa gtgtcatttt ttactgcatt tgcaaatgta ttggcatcaa tggacattga
     5701 tgcaccatca aacgctgtac cgtcactggt gaatgttcct ttatcagttt ggatggtcgc
     5761 ggctttagca ccagtagcag tagcaagttt agccaaagta gcttgtgtat caccaccagc
     5821 attattttta gttaagttac cggtagcatc catataaagt actgcgccat catcagcagc
     5881 agttaaatta ccggatttat cgatgtttac atcttgatct ttgccaccaa ttgtaactgt
     5941 agctttagtc gtatctccgg cctgaggatt gaggaaagca gccaggttag cgctggaaat
     6001 atcatcagaa gcggcggaat atgaattagt gtctttattg taggtatagg ttacagcagc
     6061 ggctggtgta ccaaaaccaa catcggcgct agttgtaact ttatttccat ctgcgccagc
     6121 aacagcattt agaatatcta cgctacttgc tacagccgat ttgtcagtat attgagttac
     6181 accaacagtt ccccctggtg ccgccgcagc tgtgaatcca gacatatctt tcagggttgc
     6241 tgccgtatta gccgtttccc ctttgccgtt cacattaaac ccattcagcc ccagcgtatc
     6301 agagtcaatt ttcttcagat caatagtgat agtctggccg tcattcgcac caacctgaat
     6361 tttcatcgaa ccgtcttttg ccagtacgtt cacgccgttg aactgggtct gaccggatac
     6421 gcggtcaatt tcgtcgagac gggatttgat ttcgtcctga atggagtcca gatccgaatc
     6481 agagttagtc ccggtagaag cctgaaccgt cagttcacga atacgctgta agttgttgtt
     6541 aatttcggac agcgcgcctt cagtggtctg tgcaacagaa ataccgtcgt tggcgttacg
     6601 tgcagcctga gtcaggcctt taatgttaga agtaaaacgg ttagcaatcg cctgacccgc
     6661 ggcgtcatcc ttcgcgctgt taatacgcaa gccagaagac agacgctcga tagaactcga
     6721 cagcgcagac tggttcttgt tgatattatt ttgagtgatc agcgagaggc tgttggtatt
     6781 aatgacttgt gccatgattc gttatcctat attgcaagtc gttgattacg ttttgggttt
     6841 ccacccgtcg gctcaatcgc cgtcaaccct gttatcgtct gtcgtaaaac aacctttaga
     6901 atttttttca caaacagcca ttttttgtta gtcgacgaaa tactcttttc tctgcccctt
     6961 attcccgcta ttaaaaaaaa caattaaacg taaactttgc gcaattcagg ccgataaccc
     7021 cggtattcgt tttacgtgtc gaaagataaa aggaaatcgc atggcaagta tttcatcgct
     7081 gggagtcggg tcaggtctgg atttaagttc aatccttgat agcctcaccg ccgcgcaaaa
     7141 agcgacgcta acccctattt caaatcagca atcgtcgttt accgctaaac ttagcgccta
     7201 cggtacgctg aaaagcgcgc tgacgacttt ccagaccgcc aatactgcat tgtctaaagc
     7261 cgatcttttt tccgctacca gcaccaccag cagcaccacc gcgttcagtg ccaccaccgc
     7321 gggtaacgcc atcgccggga aatacaccat cagcgtcacc catctggcgc aggcgcaaac
     7381 cctgaccacg cgcaccacca gagacgatac gaaaacggcg atcgccacca gcgacagcaa
     7441 actcaccatt caacaaggcg gcgacaaaga tccgatttcc attgatatca gcgcggctaa
     7501 ctcgtcttta agcgggatcc gtgatgccat caacaacgca aaagcaggcg taagcgcaag
     7561 catcattaac gtgggtaacg gtgaatatcg tctgtcagtc acatcaaatg acaccggcct
     7621 tgataatgcg atgacactct cggtcagcgg tgatgatgcg ctacaaagtt ttatgggcta
     7681 tgacgccagt gccagcagca acggcatgga ggtctcggtc gccgcccaga atgcacagct
     7741 gacggtcaat aacgtcgcca ttgagaacag cagcaacacc atcagcgacg cgctggaaaa
     7801 catcaccctg aacctgaacg atgtcaccac aggcaaccag acgctgacca tcactcagga
     7861 cacctccaaa gcgcaaacgg cgattaaaga ctgggtgaat gcctataact cgctaataga
     7921 taccttcagc agcctgacca aatacaccgc cgtagatgcg ggagctgata gccagaattc
     7981 tagcaatggc gcactgctcg gcgactccac gctgcggacg attcagacgc agttgaaatc
     8041 gatgctgagt aataccgtca gttcttccaa ctataaaacg ctggcgcaga ttggtatcac
     8101 gaccgatccc agcgatggca aactggaact ggatgccgac aaactcaccg ctgcattgaa
     8161 aaaagatgcc agcggcgtag gtgcattaat tgttggcgat ggtaaaaaaa ccggcatcac
     8221 gaccaccatc ggcagcaacc tgaccagttg gctttcgaca acgggcatta ttaaagccgc
     8281 taccgatggc gttagtaaga ccctgaataa gttaactaaa gactacaacg ccgccagcga
     8341 tcgcattgat gcgcaggtcg cgcgctataa agaacaattt acccaactgg acgttttaat
     8401 gacctcgtta aacagcacca gcagctactt aacgcagcag ttcgaaaaca acagtaattc
     8461 caagtaatca atattcatcg ggagacaggt catgtacgcg gcaaaaggca cccaggccta
     8521 tgcacaaatt ggcgtcgaaa gcgccgtaat gagcgccagc caacagcagt tggtcaccat
     8581 gctatttgat ggagtgctga gcgcactggt tagagcgcgc ctgtttatgc aggacaacaa
     8641 tcagccaggc aaaggtgtct ctttgtcaaa agcgatcaac atcattgaga acggactgcg
     8701 ggtgagtctt gatgaagaga gcaaagacga attaacccaa aacttaattg ctctttatag
     8761 ctatatggtc aggcgtttgc tgcaagccaa tttacgcaac gatgtctccg cagtcgaaga
     8821 ggtggaagca ttaatgcgta atattgccga tgcctggaaa gagtctttac tctccccttc
     8881 tttgattcag gacccagtct gatgaacaat gcaccgcatt tgtatttcgc ctggcaacaa
     8941 ctcgtcgaaa aaagccagct catgttacgc ctggcaacgg aagaacaatg ggacgaactc
     9001 atcgccagcg aaatggcgta tgtgaatgcg gtgcaggaga tcgcacacct gactgaagag
     9061 gttgccccgt ccactacgat gcaggagcag ctccgcccga tgctgcacct gattctcgac
     9121 aacgaaagca aggtaaagca gttattacag attcggatgg atgaactggc gaaactggtc
     9181 ggtcagtcat cggtgcaaaa atcggtgtta agtgcctatg gcgatcaggg cggctttgtg
     9241 ctggctccgc aggataacct cttttgaatc ttaatgagcc gatggctcgc gaataatccg
     9301 attacggcta cgcttctaat attccccttg aatggagtcg aagaatgcgt aatcccacgc
     9361 tgttacaatg ttttcactgg tattacccgg aaggcggtaa gctctggcct gaactggccg
     9421 agcgcgcaga cggttttaat gatattggta tcaatatggt ctggttgccg cccgcctata
     9481 aaggcgcatc gggcgggtac tcggtcggct acgactccta tgatttattt gatttaggcg
     9541 agtttgatca gaaaggcagc atccctacta aatatggcga taaagcacaa ctgctggccg
     9601 ccattgacgc cctgaaacgt aatgacattg cggtgctgct ggacgtggta gtcaaccaca
     9661 aaatgggcgc ggatgaaaaa gaagctattc gcgtgcaacg tgtaaatgct gatgaccgta
     9721 cgcaaattga cgaagaaatc attgagtgcg aaggctggac gcgttacacc ttccccgcgc
     9781 gtgccgggca atactcgcag tttatctggg atttcaaatg ttttagcggt atcgaccata
     9841 tcgaaaaccc tgatgaagat ggcatattta aaattgttaa cgactacacc ggcgaaggct
     9901 ggaacgatca ggttgatgat gaactcggta atttcgatta tctgatgggc gagaatatcg
     9961 attttcgcaa tcatgccgtg acggaagaga ttaaatactg ggcgcgctgg gtgatggaac
    10021 aaacacaatg cgacggtttt cgtcttgatg cggtcaaaca tattccggcg tggttttata
    10081 aagagtggat cgaacacgta caagaagttg cgccaaagcc gctgtttatt gtggctgaat
    10141 actggtcgca tgaagtcgat aaactgcaaa cgtatattga tcaggtggaa ggcaaaacca
    10201 tgctgttcga tgcaccgctg cagatgaaat tccatgaagc atcgcgcatg gggcgcgact
    10261 acgacatgac gcagattttc acgggtacat tagtggaagc cgatcctttc cacgccgtga
    10321 cgctcgttgc caatcacgac acccaaccgt tgcaagccct cgaagcgccg gtcgaaccgt
    10381 ggtttaaacc gctggcgtat gccttaattt tgttgcggga aaatggcgtt ccttcggtgt
    10441 tctatccgga cctctacggt gcgcattacg aagatgtcgg tggtgacggg caaacctatc
    10501 cgatagatat gccaataatc gaacagcttg atgagttaat tctcgcccgt cagcgtttcg
    10561 cccacggtgt acagacgtta tttttcgacc atccgaactg cattgccttt agccgcagtg
    10621 gcaccgacga atatcccggc tgcgtggtgg tcatgtcgaa cggggatgat ggcgaaaaaa
    10681 ccattcatct gggagagaat tacggcaata aaacctggcg tgattttctg gggaatcggc
    10741 aagagagcgt agtgaccgac gaaaacggcg aagcgacctt cttttgcaac ggcggcagcg
    10801 tcagcgtgtg ggttatcgaa gaggtgattt gaatccatcc ccggcggcaa gccggggaga
    10861 tttcattacg gcagtggcgt cggtaacggc gctttatcca gcgcggcggc acactctgcc
    10921 gtcgggcgat ccacacgctc catcgtcata ccatcgtact caatggtgtt accgtcacgc
    10981 tcaacctcgt acagctcacg ttttacggta acgttagtga gatcatcaga catcaatgtc
    11041 aacttacccg gcacggcaat cacccgctgc cactgacggc aatccagggt atcgccctct
    11101 ttagtgacaa tgaggctgcc tatcgcttcc ggactgacca acgcacgttg cggccctttg
    11161 gtctgccagt aacccgtcag ccagtccggc gcgggggttt tcacgacatt gttatagttt
    11221 tcgacttcgg cacagcccgc cagcaacatg agcgcacctg caattgcgag ttttttcatc
    11281 attcgtcctg catgagaaga aaagaggatt gtggcattaa agccctgatg tcgccagcct
    11341 ttccaggttc agagagaaat ttgatctaac cattattttt gtgaattatt accccgaaaa
    11401 taaattttca tggtgtatga ttcgcgactc ttgtttcaaa ccttctgagt tcagaggcta
    11461 cattcatgtc atggcagcaa ttcaaacacg cctggttgat taaattctgg gcacccatcc
    11521 ctgcggtcat cgcggcgggt attctctcca cttactattt tggcatcact ggcacctttt
    11581 gggcagtcac gggtgaattt acccgttggg gcggtcagct tttgcagctg tttggcgtcc
    11641 atgctgaaga gtggggttat tttaaaatta tccatctgga aggatcgcca ttaacccgca
    11701 tcgacggaat gatgatcctc ggtatgtttg gcggctgttt tgccgcagcg ctgtgggcca
    11761 acaatgtcaa actgcgcatg ccgcgcagcc gtatccgcat tatgcaggcc atcattggcg
    11821 gtattatcgc cggttttggc gcacgtctgg caatgggctg taacctggcg gcgttcttta
    11881 ccggaattcc acagttctcg ctgcacgcct ggttctttgc catcgccact gccattggtt
    11941 catggtttgg cgcgcgcttt accctgctgc ccatcttccg tattcccgtg aaaatgcaga
    12001 aagtttctgc tgcctcaccg ttgacgcaaa aaccggatca ggcgcggcgt cgttttcgtc
    12061 tcgggatgct ggtctttttc ggcatgctgg gctgggcgct gctgacggcg atgaaccagc
    12121 ccaaactggg gctggcaatg ctgtttggcg tcggctttgg tttactgatt gaacgtgcgc
    12181 aaatctgctt tacttcggcg ttccgcgata tgtggatcac cggacgtacc catatggcga
    12241 aagcaatcat tatcggtatg gcggtaagtg ccatcgggat cttcagttac gtacagttag
    12301 gcgttgaacc caaaatcatg tgggcgggac caaacgcggt aattggtggt ttactgtttg
    12361 gttttggcat cgtgctggca ggcggctgcg aaaccggctg gatgtaccgc gcggtagagg
    12421 gccaggtaca ctactggtgg gtcggtctgg gcaacgtgat cggctcaacg attctggcgt
    12481 actactggga tgatttcgct ccggcgctgg ccaccgactg ggacaaaatc aacctgctga
    12541 aaacctttgg cccgatgggc ggcctgctgg tgacatattt gctgttgttt gcagctctaa
    12601 tgttgattat cggctgggaa aaacgcttct tccgccgtgc ggcaccgcag attgctaagg
    12661 agatcgcatg aaaaatatcg ttcctgatta ccgtcttgat atggtgggtg aaccctgccc
    12721 ttatccggca gtcgcaaccc ttgaggcgat gccgcagtta aaaaaagggg agatcctgga
    12781 agtggtgagc gactgtccgc agtcgatcaa taatattcca ctggatgcgc gtaatcacgg
    12841 ctatacggtg ctggatattc agcaagacgg gccgaccatt cgttatttaa ttcagaagta
    12901 atccctcatt cccgctgtgg cccacagcgg gaacatcgtt acctctctat cacatagatt
    12961 ttacccgccc cctctcctat tcttttcagt gtgacctcag gaggtttcta tgtgtggacg
    13021 ctttgcccaa tcccaaacgc gtgaagatta ccttgcgctt ctcgcggaag atattgaacg
    13081 cgatattccc tacgatcccg aacccattgg cagatacaac gtcgcgccgg gaaccaaagt
    13141 tcttctgctc agtgaacgtg atgaacacct tcatctggat ccggttttct ggggatatgc
    13201 tcccggatgg tgggataaac cgccgctgat taacgcccgc gtagaaactg ccgcaaccag
    13261 tcgtatgttt aaaccgctct ggcaacatgg tcgggcaatc tgttttgccg atggctggtt
    13321 tgagtggaaa aaagaaggcg acaaaaaaca gccttatttt atctatcgcg ctgatggaca
    13381 acctgttttt atagccgcga taggtagcac accgtttgag cgtggtgacg aagccgaagg
    13441 atttttaatt gtcactgcag cagcagacca gggtctggta gatattcatg accgccgccc
    13501 gctggtactg tcgccagaag ctgcacggga atggatgcgg caagagatta gcggtaaaga
    13561 agcctctgaa attgctgcca gtggctgtgt tccagcaaac cagttcacct ggcacccggt
    13621 atcgtgcgcg gtgggtaatg ttaaaaacca gggcgcggag ttaattcaac ctgtttgata
    13681 agcctggaga ttatctacac ctagatggac atcattgtac tatgccccgt acatgcccca
    13741 tcacatcctc aacatcaaag tattagagcc ttgcaaacca ttatgcgtct cagtttgtct
    13801 catctatctt acgc
//
LOCUS       DADSYM010000126        13798 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2415
            SAMN08039579-rid11723173.denovo.127, whole genome shotgun sequence.
ACCESSION   DADSYM010000126 DADSYM010000000
VERSION     DADSYM010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039579
            Sequence Read Archive: SRR6362533
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13798)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13798)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980162.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 03:39:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,484
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 4,347
            CDSs (with protein)               :: 4,347
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 43 of 85
            Pseudo Genes (incomplete)         :: 37 of 85
            Pseudo Genes (internal stop)      :: 19 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13798
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039579-rid11723173.denovo.127"
                     /strain="LGE2415"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8302 N 95.9628 W"
                     /collection_date="2015-08-24"
     gene            complement(2..94)
                     /locus_tag="J1Y83_003350"
     tRNA            complement(2..94)
                     /locus_tag="J1Y83_003350"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(58..60),aa:Ser,seq:gct)
     gene            complement(410..595)
                     /gene="csrA"
                     /locus_tag="J1Y83_003351"
     CDS             complement(410..595)
                     /gene="csrA"
                     /locus_tag="J1Y83_003351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon storage regulator CsrA"
                     /protein_id="HBA7631467.1"
                     /translation="MLILTRRVGETLMIGDEVTVTVLGVKGNQVRIGVNAPKEVSVHR
                     EEIYQRIQAEKSQQSSY"
     gene            complement(830..3460)
                     /gene="alaS"
                     /locus_tag="J1Y83_003352"
     CDS             complement(830..3460)
                     /gene="alaS"
                     /locus_tag="J1Y83_003352"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HBA7631468.1"
                     /translation="MSKSTAEIRQAFLDFFHSKGHQVVASSSLVPHNDPTLLFTNAGM
                     NQFKDVFLGLDKRNYSRATTSQRCVRAGGKHNDLENVGYTARHHTFFEMLGNFSFGDY
                     FKHDAIQFAWELLTSEKWFALPKERLWVTVYESDDEAYEIWEKEVGIPRERIIRIGDN
                     KGAPYASDNFWQMGDTGPCGPCTEIFYDHGDHIWGGPPGSPEEDGDRYIEIWNIVFMQ
                     FNRQADGTMEPLPKPSVDTGMGLERIAAVLQHVNSNYDIDLFRTLIQAVAKVTGATDL
                     SNKSLRVIADHIRSCAFLIADGVMPSNENRGYVLRRIIRRAVRHGNMLGAKETFFYKL
                     VGPLIDVMGSAGEDLKRQQAQVEQVLKTEEEQFARTLERGLALLDEELAKLSGDTLDG
                     ETAFRLYDTYGFPVDLTADVCRERNIKVDEAGFEAAMEEQRRRAREASGFGADYNAMI
                     RVDSASEFKGYDHLELNGKVTALFVDGKAVDAINAGQEAVVVLDQTPFYAESGGQVGD
                     KGELKGANFSFAVEDTQKYGQAIGHIGKLAAGSLKVGDAVQADVDEARRARIRLNHSA
                     THLMHAALRQVLGTHVSQKGSLVNDKVLRFDFSHNEAMKPEEIRAVEDLVNAQIRRNL
                     PIETNIMDLEAAKAKGAMALFGEKYDERVRVLSMGDFSTELCGGTHASRTGDIGLFRI
                     ISESGTAAGVRRIEAVTGEGAIATVHADSDRLSEVAHLLKGDSNNLADKVRSVLERTR
                     QLEKELQQLKEQAAAQESANLSSKAIDVNGVKLLVSELSGVEPKMLRTMVDDLKNQLG
                     STIIVLATVAEGKVSLIAGVSKDVTDRVKAGELIGMVAQQVGGKGGGRPDMAQAGGTD
                     AAALPAALASVKGWVSAKLQ"
     gene            complement(3589..4089)
                     /gene="recX"
                     /locus_tag="J1Y83_003353"
     CDS             complement(3589..4089)
                     /gene="recX"
                     /locus_tag="J1Y83_003353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HBA7631469.1"
                     /translation="MTESTSRRPAYARLLDRAVRILAVRDHSEQELRRKLAAPIMGKN
                     GPEEIDATAEDYERVIAWCHEHGYLDDSRFVARFIASRSRKGYGPARIRQELNQKGIS
                     REATEKAMRECDIDWCALARDQATRKYGEPLPTVFSEKVKIQRFLLYRGYLMEDIQDI
                     WRNFAD"
     gene            complement(4158..5219)
                     /gene="recA"
                     /locus_tag="J1Y83_003354"
     CDS             complement(4158..5219)
                     /gene="recA"
                     /locus_tag="J1Y83_003354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HBA7631470.1"
                     /translation="MAIDENKQKALAAALGQIEKQFGKGSIMRLGEDRSMDVETISTG
                     SLSLDIALGAGGLPMGRIVEIYGPESSGKTTLTLQVIAAAQREGKTCAFIDAEHALDP
                     IYARKLGVDIDNLLCSQPDTGEQALEICDALARSGAVDVIVVDSVAALTPKAEIEGEI
                     GDSHMGLAARMMSQAMRKLAGNLKQSNTLLIFINQIRMKIGVMFGNPETTTGGNALKF
                     YASVRLDIRRIGAVKEGENVVGSETRVKVVKNKIAAPFKQAEFQILYGEGINFYGELV
                     DLGVKEKLIEKAGAWYSYKGEKIGQGKANATAWLKDNPETAKEIEKKVRELLLSNPNS
                     TPDFSVDDSEGVAETNEDF"
     gene            complement(5299..5796)
                     /gene="pncC"
                     /locus_tag="J1Y83_003355"
     CDS             complement(5299..5796)
                     /gene="pncC"
                     /locus_tag="J1Y83_003355"
                     /EC_number="3.5.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide-nucleotide amidase"
                     /protein_id="HBA7631471.1"
                     /translation="MTDSELMQLSEQVGQALKVRGATVTTAESCTGGWVAKVITDIAG
                     SSAWFERGFVTYSNEAKAQMIGVREETLAQHGAVSEPVVVEMAIGALKAARADYAVSI
                     SGIAGPDGGSEEKPVGTVWFAFATARGEGITRRECFSGDRDAVRRQATAYALQTLWQQ
                     FLQNT"
     gene            complement(5941..7026)
                     /gene="mltB"
                     /locus_tag="J1Y83_003356"
     CDS             complement(5941..7026)
                     /gene="mltB"
                     /locus_tag="J1Y83_003356"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic murein transglycosylase B"
                     /protein_id="HBA7631472.1"
                     /translation="MFKRRYVTLLPLFVLLAACSSKPKPTETETTTGTPSGGFLLEPQ
                     HNVMQMGGDFANNPNAQQFIDKMVNKHGFDRQQLQEILSQAKRLDSVLRLMDNQAPTT
                     SVKPPSGPNGAWLRYRKKFITPDNVQNGVIFWNQYEDALNRAWQVYGVPPEIIVGIIG
                     VETRWGRVMGKTRILDALATLSFNYPRRAEYFSGELETFLLMARDEQDDPLNLKGSFA
                     GAMGYGQFMPSSYKQYAVDFSGDGHINLWDPVDAIGSVANYFKAHGWVKGDQVAVMAN
                     GQAPGLPNGFKTRYSISQLAAAGLTPQQPLGNHQQASLLRLDVGTGYQYWYGLPNFYT
                     ITRYNHSTHYAMAVWQLGQAVALARVQ"
     gene            7282..7845
                     /gene="srlA"
                     /locus_tag="J1Y83_003357"
                     /gene_synonym="gutA"
     CDS             7282..7845
                     /gene="srlA"
                     /locus_tag="J1Y83_003357"
                     /gene_synonym="gutA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
                     /protein_id="HBA7631473.1"
                     /translation="MIETITHGAEWFIGLFQKGGEVFTGMVTGILPLLISLLVIMNAL
                     INFIGQHRIERFAQRCAGNPVSRYLLLPCIGTFVFCNPMTLSLGRFMPEKYKPSYYAA
                     ASYSCHSMNGLFPHINPGELFVYLGIASGLTTLNLPLGPLAVSYLLVGLVTNFFRGWV
                     TDLTTAIFEKKMGIQLEQKVRLAGATS"
     gene            7842..8801
                     /gene="srlE"
                     /locus_tag="J1Y83_003358"
                     /gene_synonym="gutE"
     CDS             7842..8801
                     /gene="srlE"
                     /locus_tag="J1Y83_003358"
                     /gene_synonym="gutE"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HBA7631474.1"
                     /translation="MTRIRIEKGTGGWGGPLELEATPGKKIVYITAGTRPAIVDKLAQ
                     LTGWQAIDGFKEGEPAEAEIGVAVIDCGGTLRCGIYPKRRIPTINIHSTGKSGPLAQY
                     IVEDIYVSGVKEENITVVGEATPQPSSVGRDYDTSKKITEQSDGLLAKVGMGMGSAVA
                     VLFQSGRDTIDTVLKTILPFMAFVSALIGIIMASGLGDWIAHGLAPLASHPLGLVMLA
                     LICSFPLLSPFLGPGAVIAQVIGVLIGVQIGLGNIPPHLALPALFAINAQAACDFIPV
                     GLSLAEARQDTVRVGVPSVLVSRFLTGAPTVLIAWFVSGFIYQ"
     gene            8812..9183
                     /gene="srlB"
                     /locus_tag="J1Y83_003359"
                     /gene_synonym="gutB"
     CDS             8812..9183
                     /gene="srlB"
                     /locus_tag="J1Y83_003359"
                     /gene_synonym="gutB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HBA7631475.1"
                     /translation="MTVIYQTTITRIGASATDALSDQMLITFREGAPADLEEYCFIHC
                     HGELKGALHPGLQFTLGRHRYPVTAVGSVAEDNLRELGHVTLRFDGLSEAEFPGTVHV
                     AGPVPDDIAPGSVLKFESVKE"
     gene            9187..9966
                     /gene="srlD"
                     /locus_tag="J1Y83_003360"
     CDS             9187..9966
                     /gene="srlD"
                     /locus_tag="J1Y83_003360"
                     /EC_number="1.1.1.140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sorbitol-6-phosphate dehydrogenase"
                     /protein_id="HBA7631476.1"
                     /translation="MNQVAVVIGGGQTLGAFLCHGLAAEGYRVAVVDIQSDKAANVAQ
                     EINAEYGEGTAYGFGADATSEQSVLALSRGVDEIFGRVDLLVYSAGIAKAAFISDFQL
                     GDFDRSLQVNLVGYFLCAREFSRLMIRDGIQGRIIQINSKSGKVGSKHNSGYSAAKFG
                     GVGLTQSLALDLAEYGITVHSLMLGNLLKSPMFQSLLPQYATKLGIKPEQVEQYYIDK
                     VPLKRGCDYQDVLNMLLFYASPKASYCTGQSINVTGGQVMF"
     gene            10072..10431
                     /gene="gutM"
                     /locus_tag="J1Y83_003361"
     CDS             10072..10431
                     /gene="gutM"
                     /locus_tag="J1Y83_003361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GutM"
                     /protein_id="HBA7631477.1"
                     /translation="MVSALITVAVIAWCAQLALGGWQISRFNRAFDTLCQQGRVGVGR
                     SSGRFKPRVVVAIALDDQQRIVDTLFMKGLTVFARPQKIPAITGMHVGDLQPDVIFPH
                     DPLSQNALSLALKLKRG"
     gene            10498..11271
                     /gene="srlR"
                     /locus_tag="J1Y83_003362"
                     /gene_synonym="gutR"
     CDS             10498..11271
                     /gene="srlR"
                     /locus_tag="J1Y83_003362"
                     /gene_synonym="gutR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucitol operon DNA-binding transcriptional
                     repressor SrlR"
                     /protein_id="HBA7631478.1"
                     /translation="MKPRQRQAAILEYLQKQGKCSVEELAQYFDTTGTTIRKDLVILE
                     HAGTVIRTYGGVVLNKEESDPPIDHKTLINTHKKELIAEAAVGFIHDGDSIILDAGST
                     VLQMVPLLSRFNNITVMTNSLHIVNALSELDNEQTILMPGGTFRKKSASFHGQLAENA
                     FEHFTFDKLFMGTDGIDLNAGVTTFNEVYTVSKAMCNAAREVILMADSSKFGRKSPNV
                     VCSLESVDKLITDAGIDPAFRQALEEKGIDVIITGESNE"
     gene            11264..12229
                     /gene="gutQ"
                     /locus_tag="J1Y83_003363"
     CDS             11264..12229
                     /gene="gutQ"
                     /locus_tag="J1Y83_003363"
                     /EC_number="5.3.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311591.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-5-phosphate isomerase GutQ"
                     /protein_id="HBA7631479.1"
                     /translation="MSEALLNAGRQTLMLELQEASRLPERLGDDFVRAANIILHCEGK
                     VVVSGIGKSGHIGKKIAATLASTGTPAFFVHPAEALHGDLGMIESRDVMLFISYSGGA
                     KELDLIIPRLEDKSIALLAMTGKPTSPLGLAAKAVLDISVEREACPMHLAPTSSTVNT
                     LMMGDALAMAVMQARGFNEEDFARSHPAGALGARLLNKVHHLMRRDDAIPQVALTASV
                     MDAMLELSRTGLGLVAVCDDQRLVKGVFTDGDLRRWLVGGGALTTPVNEAMTVGGTTL
                     QSQSRAIDAKEILMKRKITAAPVVDENGKLTGAINLQDFYQAGII"
     gene            complement(12226..13740)
                     /gene="norR"
                     /locus_tag="J1Y83_003364"
     CDS             complement(12226..13740)
                     /gene="norR"
                     /locus_tag="J1Y83_003364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide reductase transcriptional regulator
                     NorR"
                     /protein_id="HBA7631480.1"
                     /translation="MSFSVDVLANIAIELQRGIGHQDRFQRLITTLRQVLECDASALL
                     RYDSRQFIPLAIDGLAKDVLGRRFALEGHPRLEAIARAGDVVRFPADSELPDPYDGLI
                     PGQESLKVHACVGLPLFAGQNLIGALTLDGMQPDQFDVFSDEELRLIAALAAGALSNA
                     LLIEQLESQNMLPGEAAPFEAVKQTQMIGLSPGMTQLKKEIEIVAASDLNVLISGETG
                     TGKELVAKAIHEASPRAVNPLVYLNCAALPESVAESELFGHVKGAFTGAISNRSGKFE
                     MADNGTLFLDEIGELSLALQAKLLRVLQYGDIQRVGDDRSLRVDVRVLAATNRDLREE
                     VLAGRFRADLFHRLSVFPLSVPPLRERGDDVILLAGYFCEQCRLRLGLSRVVLSAGAR
                     NLLQHYNFPGNVRELEHAIHRAVVLSRATRSGDEVILEAQHFAFPEVTLPPPEAAAVP
                     VVKQNLREATEAFQRETIRQALAQNHHNWAACARMLETDVANLHRLAKRLGLKD"
BASE COUNT         3197 a         3827 c         3460 g         3314 t
ORIGIN      
        1 atggcggtga ggcggggatt cgaaccccgg atgcagcttt tgaccgcata ctcccttagc
       61 aggggagcgc cttcagcctc tcggccacct caccacacgc ctcttacgag tgcttcgaag
      121 aacttgttta tcgctcatcg tcgctgcgtg gcgcacatat tactttctga gacttataag
      181 tcaaacaatt tttcccacac ttttatcgtt tgcacacttc acgctcaatt agtctgtaaa
      241 aacgtcaaaa agagtgtttt atcaacagaa aaacggaggt ctgacagata gcagtaatgc
      301 aaaaaaatgg agacttaagt tgaatgaacg ggagtaaagc gaaaagacaa tggagtgaaa
      361 gagaaatttt gagggtgcgt ctcaccgata aagatgagac gcggaaagat tagtaactgg
      421 actgctggga tttttcagcc tggatacgct ggtagatctc ttcacggtga acagaaactt
      481 ccttcggggc atttacgcca atacgtacct ggttgccctt tacccctaaa actgtcacgg
      541 tgacctcatc cccaatcatg agggtctcac caactcgacg agtcagaatc agcattcttt
      601 gctccttgaa agattaaaag agtcgggtct ctctgtatcc cggcattatc catcatataa
      661 cgccaaaaag taagcaatga caaacacatt acatctaagc agtcatggca ttacattctg
      721 ttaaacctaa gtttagccga tatacacaac ttcaacctga ctttatcgtt gtcgatagcg
      781 ttaatgcgaa tgccgtgaag cgagtccacg gcattgcctg acgcttatat tattgcaatt
      841 tcgcgctgac ccagcctttc acactggcta acgctgcagg taaggccgca gcatccgtac
      901 caccggcttg cgccatgtca ggacgtccac cacccttgcc gcccacctgc tgagcgacca
      961 taccaatcag ttcccctgct ttcacacgat ctgtgacgtc cttagatacg cctgcaatca
     1021 gagaaacctt accttcggct accgttgcca gcacgataat tgtcgacccc agctgatttt
     1081 ttaaatcgtc aaccatggta cgcaacattt tcggctcaac accgctaagc tcgctaacca
     1141 acagcttaac accattaaca tcaattgcct tactggaaag atttgcgctc tcctgtgcgg
     1201 cagcttgttc tttaagctgt tgtaactctt tttccagctg acgcgtacgt tccagtactg
     1261 aacgcacttt atcagccaga ttattgctat cgcctttcag cagatgcgcg acttcgctta
     1321 agcgatcact gtctgcatga acggtggcga tagcgccttc tccggttacc gcttcgatac
     1381 gacgaacgcc tgcagcagta cccgattcag agatgatgcg gaacagacca atatcaccag
     1441 tgcggctggc gtgagtaccg ccacacagct cggtggagaa atcgcccatg ctcagcacgc
     1501 gtacgcgctc atcatacttc tcgccaaaca gcgccatagc acctttcgct ttcgccgctt
     1561 cgagatccat gatgttggtt tcgatcggca agttacggcg aatctgtgcg ttcaccaggt
     1621 cttcgaccgc acgaatctct tctggtttca tcgcttcgtt gtgtgagaag tcgaagcgca
     1681 gcactttgtc gttaaccagt gaacctttct gcgatacatg agtacccaga acctggcgca
     1741 gcgcagcgtg catcaggtgc gttgcggagt gattcagacg aatacgggcg cgacgagcct
     1801 catcaacatc agcctgcaca gcgtcgccca ctttcagaga acccgcagca agtttaccga
     1861 tgtggccaat cgcctggccg tatttctgcg tatcttccac cgcgaaggag aagttagcgc
     1921 ctttcagttc gcctttatca ccaacctgac cgccggattc cgcatagaat ggcgtttgat
     1981 ccagcacgac cacagcttcc tggcctgcat tgatggcatc aaccgcttta ccatcaacaa
     2041 acagcgcggt cactttgccg ttcagttcca gatggtcata gcctttaaat tcagatgcac
     2101 tgtcaacacg gatcattgcg ttgtaatcgg caccaaagcc gctggcttcg cgcgcacgac
     2161 gacgctgctc ttccattgct gcttcaaaac cagcttcgtc aactttgatg ttgcgctcac
     2221 gacaaacatc agccgtcagg tcaaccggga agccgtaggt gtcgtacagg cggaaagcag
     2281 tttcaccatc cagcgtatca ccagaaagtt ttgccagctc ttcatccagc agcgccagac
     2341 cgcgctccag agtacgggca aactgctctt cttcagtctt cagcacctgc tcaacctgcg
     2401 cctgctggcg tttcaggtct tcacccgcag agcccataac gtcgatcagc ggaccaacca
     2461 gtttgtagaa gaaggtttct ttcgcgccga gcatattgcc gtgacgcact gcgcgacgaa
     2521 tgatacgacg cagtacataa ccacggtttt cgttggacgg cattacgcca tccgcgatca
     2581 ggaacgcaca agaacgaatg tggtcagcga ttacgcgcag cgatttattg ctcagatcgg
     2641 ttgcgccagt gactttcgct accgcctgga tcagcgtgcg gaacaggtcg atgtcatagt
     2701 tagagttaac gtgttgcagc accgcagcaa tacgctccag acccataccg gtatctacag
     2761 acggcttcgg cagcggttcc atcgtgccat cggcctggcg gttgaactgc atgaagacga
     2821 tgttccagat ctcaatgtag cggtcgccgt cttcttccgg acttcccgga gggccacccc
     2881 aaatgtggtc gccgtgatcg tagaagattt cggtgcacgg gccgcacgga ccagtgtcac
     2941 ccatctgcca gaagttgtca gatgcgtatg gcgcaccttt gttatcgccg atacgaataa
     3001 tacgttcgcg cgggatccct acttcttttt cccagatttc gtaggcttcg tcgtcgcttt
     3061 catagacggt aacccacaga cgctctttcg gcagggcaaa ccatttttcg ctggtcagca
     3121 gttcccatgc aaactgaatg gcatcgtgtt tgaaatagtc gccgaagctg aagttgccca
     3181 gcatttcgaa gaaggtatga tgacgcgcgg tgtaaccgac gttttccagg tcgttgtgtt
     3241 taccacccgc acgcacgcag cgttgggaag tggtagcgcg ggaataatta cgcttgtcga
     3301 gcccaaggaa cacatccttg aactggttca tcccggcgtt ggtaaacaac aaagttgggt
     3361 cgttatgggg taccagggag ctgctggcaa ctacctgatg tcccttacta tggaaaaagt
     3421 cgagaaacgc ctgacggatc tcagcggtgc tcttgctcat aattatcctg aaatcaagct
     3481 aacgaaatat cgctaccagc cgttgcgtgt cgttaaccgc ccggctggta actgaaaagt
     3541 gggaataaga taagttttct tgactgggaa gtaaaatccc gtatgcgttc agtcggcaaa
     3601 atttcgccaa atatcctgga tatcttccat cagatagcca cgatagagca gaaaacgctg
     3661 gatcttaact ttttctgaaa agacagttgg caaaggttcg ccatattttc gcgtcgcctg
     3721 atcgcgcgcc agtgcgcacc agtcgatgtc acattcacgc atcgcttttt ctgtcgcttc
     3781 gcgggaaata cctttctgat tcagttcctg acgaatacgc gcaggtccat aacctttgcg
     3841 gctacggctg gcgataaagc gcgcaacaaa tcggctgtca tcaagatagc catgttcatg
     3901 gcaccaggca ataacgcgct cgtaatcttc tgccgtggca tcaatctctt ccgggccatt
     3961 tttgcccata atcggtgccg cgagtttacg tcgcagttct tgctcactgt gatcgcgcac
     4021 cgccagaatg cgtaccgcac gatccaacag gcgagcatat gccgggcgac gggatgttga
     4081 ttctgtcatg gcatatcctt acaacttaaa aaagcaaaag ggccgcagat gcgacccttg
     4141 tgtatcaaac aagacgatta aaaatcttcg ttagtttctg ctacgccttc gctgtcatct
     4201 acagagaaat ccggcgttga gttcgggttg ctcagcagca actcacgtac tttcttctcg
     4261 atctctttcg cggtttccgg gttatctttc agccaggcag tcgcattcgc tttaccctga
     4321 ccgatcttct cacctttgta gctgtaccac gcgcctgctt tctcgataag cttctctttc
     4381 acgcccaggt caaccagctc gccgtagaag ttgatacctt cgccgtagag gatctggaat
     4441 tcagcctgtt taaacggcgc agcgattttg ttcttcacca ctttcacgcg ggtttcgcta
     4501 cccaccacgt tttcgccctc tttcaccgcg ccgatacgac ggatgtcgag acgaacagag
     4561 gcgtagaatt tcagcgcgtt accaccggta gtggtttccg ggttaccgaa catcacacca
     4621 attttcatac ggatctggtt gatgaagatc agcagcgtgt tggactgctt caggttaccc
     4681 gccagcttac gcatcgcctg gctcatcata cgtgccgcaa ggcccatgtg agagtcgccg
     4741 atttcgcctt cgatttccgc tttcggcgtc agtgccgcca cggagtcaac gacgataacg
     4801 tctactgcgc cagaacgtgc cagggcatca cagatttcca gtgcctgctc gccggtatcc
     4861 ggctgggagc acagcaggtt gtcaatatcg acgcccagtt tacgtgcata gattgggtcc
     4921 agcgcgtgtt cagcatcgat aaacgcacag gttttacctt cgcgctgcgc tgcggcgatc
     4981 acctgcaacg tcagcgtggt tttaccggaa gattctggtc cgtagatttc gacgatacgg
     5041 cccatcggca gaccacctgc cccaagcgcg atatccagtg aaagcgaacc ggtagagatg
     5101 gtttccacat ccatggaacg gtcttcaccc aggcgcatga tggagccttt accaaattgt
     5161 ttctcaatct ggcccagtgc tgccgccaac gctttctgtt tgttttcgtc gatagccatt
     5221 tttactcctg tcatgccggg taataccgga tagtcaatat gttctgttga agcaattata
     5281 ctgtatgctc atacagtatc aagtgttttg tagaaattgt tgccacaagg tctgcaatgc
     5341 atacgcagta gcctgacgac gcaccgcatc acggtcaccg ctgaagcatt cccggcgggt
     5401 aataccttcg ccgcgggcgg tggcaaaagc aaaccagacg gtgccgacag gcttctcttc
     5461 actgccgcca tccggcccgg cgataccact aatagacacg gcataatcag cacgagccgc
     5521 tttcagtgcg cctatcgcca tttccaccac gacaggctcg ctcaccgcgc catgctgcgc
     5581 cagcgtctct tcgcgtacac cgatcatttg cgctttcgct tcgttactgt aggtgacaaa
     5641 tccgcgttca aaccaggcgg agctaccggc aatatcggta atcactttcg ctacccaacc
     5701 gccggtacaa gactcagcag ttgttacggt tgcgccacgg actttcagcg cctgcccaac
     5761 ctgttcgctt aactgcatca gttcactgtc agtcatcaca acctctgtca gtcataaatt
     5821 tatgccggac aagatagcac tttcgtcagg aagatggtgt tggcgttgca attttacgag
     5881 gaggattcag aaaaaagctg attagccaga gggaagctca cgcccccctc tggcaaatag
     5941 ttactgcacg cgcgccagcg ccacggcttg tcctaactgc cagaccgcca ttgcgtaatg
     6001 ggtgctgtgg ttataacggg taatggtgta gaagttcggc agaccgtacc agtactggta
     6061 accggtaccg acatccagac gcagcaggct ggcttgttga tggttgccca gcggctgctg
     6121 tggcgttaaa cctgccgcgg caagctgcga aatgctgtac ctggttttga agccatttgg
     6181 caagcctggc gcctgaccgt ttgccattac cgcgacctga tcgcctttca cccagccgtg
     6241 cgctttgaaa taattcgcca cgctaccgat cgcatcaacc ggatcccaca ggttgatatg
     6301 cccgtcgccg ctgaaatcta ccgcgtattg tttgtaagac gacggcataa actgtccgta
     6361 gcccatcgcc ccggcaaagg aacctttcag attgagcgga tcgtcctgct cgtcgcgcgc
     6421 catcagcagg aaggtttcca gctcgccaga aaaatactcc gcgcggcgtg ggtagttaaa
     6481 tgacagcgtt gccagcgcat cgaggatgcg agttttcccc atcacgcgcc cccagcgggt
     6541 ttcaacgccg ataatcccga cgataatttc cggcggtacg ccatacacct gccacgcgcg
     6601 attcaacgca tcttcatact gattccagaa aatcacacca ttctgcacgt tgtccggcgt
     6661 aataaatttt ttgcgataac gcagccatgc gccgttcgga cctgatggcg gtttcaccga
     6721 tgtggttggt gcctggttat ccatcagccg cagtaccgaa tccagacgtt tcgcctgaga
     6781 gagaatttcc tgcaactgct gacgatcgaa accgtgtttg ttcaccattt tgtcgatgaa
     6841 ctgctgggca ttcgggttat tggcgaagtc accgcccatc tgcatcacat tgtgctgcgg
     6901 ctcaagcagg aagccgccag acggcgttcc ggtggtcgtt tcagtctcag taggttttgg
     6961 cttgctgcta caggcggcaa gcaacacaaa aaggggaagc aatgttacat aacgacgctt
     7021 gaacatgagg ggtccattta acagattcaa ccaggggcaa gtatggtaaa gcatcacgcc
     7081 ccgcacaagg aagcggtagt cactgcccga tacggacttt acataactca actcattctc
     7141 ctcgctatcc ttttattcaa actttcaaat taaaatattt atctttcatt ttgcgatcaa
     7201 aataacactt ttaaatcttt cgatctgatt agattaggtt gccgtttggt aataaaacaa
     7261 taaatactga aggagagaac aatgatagaa accattactc atggcgcaga gtggttcatc
     7321 gggctattcc agaaaggcgg agaagtgttt accgggatgg taaccggcat tcttccgctg
     7381 ttaattagcc tgctggttat catgaacgca ttgattaatt ttatcggtca gcatcgtatt
     7441 gaacgatttg ctcaacgttg cgccggtaac cctgtttccc gttatctgct gctaccgtgc
     7501 attggtacgt ttgtcttttg taacccgatg accttaagtc ttggtcgctt tatgccggaa
     7561 aagtacaaac ccagctatta cgcggcggcc tcttatagct gccactcaat gaatggcctc
     7621 ttcccccata tcaaccctgg cgaactgttt gtttatctcg gcattgccag cggtctgaca
     7681 acactgaatc tgccacttgg cccactggcg gtgagctatc tgctggttgg tctggtcacc
     7741 aacttcttcc gcggctgggt aaccgatctg accaccgcca ttttcgagaa aaagatgggc
     7801 attcaacttg aacaaaaagt tcgtctggca ggagcaacat catgacacgc attcggatcg
     7861 aaaaaggaac gggtggctgg ggcggcccgc ttgagctgga agccacgccg ggcaaaaaaa
     7921 tcgtctatat caccgccggt acccgacctg cgattgttga caaactggca cagcttactg
     7981 gctggcaagc tatcgacgga tttaaagaag gtgaacccgc ggaggcggaa attggtgtcg
     8041 cggtaatcga ctgtggcggc acattacgct gcggcatcta tccgaaacgg cgtattccca
     8101 ccattaatat ccactcgacg ggcaagtccg gcccgctggc gcagtacatt gtggaagata
     8161 tttatgtctc tggcgtaaaa gaagaaaaca tcactgtagt gggtgaggcg acaccacaac
     8221 cctcttccgt gggccgtgac tatgacacca gcaagaaaat caccgaacaa agcgatggtt
     8281 tactggcgaa ggtgggaatg ggtatgggtt ctgccgttgc cgtgctgttc cagtctggcc
     8341 gtgacaccat cgacactgta ttaaaaacca ttctgccgtt tatggcgttc gtctcggcgc
     8401 ttatcggaat cattatggct tctggccttg gtgactggat tgcccacggt cttgctccgc
     8461 tggcgagcca tccactgggt ctggtcatgc tggcgctcat ctgctccttc ccgctgcttt
     8521 cacctttcct cggcccaggc gcagttatcg cacaggttat cggcgtattg attggcgtgc
     8581 agattggtct cggcaatatt ccgccgcatc tggctttacc tgcactgttt gccatcaacg
     8641 cgcaggcggc ctgcgacttc atcccggtcg gtttgtcgct ggcggaagct cgtcaggaca
     8701 cggttcgcgt cggtgtccct tctgttctgg tgagccgctt tttaaccggc gcgccaactg
     8761 tactgatcgc ctggtttgtc tccggtttta tctatcaata gaggctgaaa catgaccgtt
     8821 atttatcaga ccaccatcac ccgtattggc gcgagtgcca ctgacgccct cagcgaccag
     8881 atgctcatca cctttcgtga aggcgcgcct gcggatctcg aagagtattg cttcattcat
     8941 tgccacggcg agttaaaagg tgcactccat cccggtttgc aatttacgct cgggcggcat
     9001 cgctatccgg tgaccgccgt tggcagcgtg gcggaagaca accttcgcga actgggtcat
     9061 gtcaccctgc gcttcgatgg tttaagcgaa gcggaatttc cgggcactgt ccatgtggca
     9121 ggccctgtac ccgacgatat cgcgccggga tcggttttga agtttgaatc tgttaaggag
     9181 taaaaaatga atcaggttgc cgttgtcatc ggtggtgggc aaaccttagg cgcgttcctg
     9241 tgccacggtc tggctgccga ggggtatcgc gtggcggttg tcgatattca gagcgacaaa
     9301 gccgcaaatg tggcacaaga aattaacgcc gaatatggtg aaggaacagc gtacggtttt
     9361 ggtgctgacg ccaccagcga gcaaagcgtt ctggcgcttt ctcgtggggt agatgaaatc
     9421 tttggtcgcg tggatttgct ggtctacagc gccggaatag caaaagcagc ctttatcagc
     9481 gacttccagc tcggcgattt tgaccgttcg ctacaggtga atctggtggg ttatttcctg
     9541 tgtgcccgtg aattttcgcg tttgatgatc cgcgacggga ttcagggtcg cattattcag
     9601 atcaactcga aatccggcaa agtgggcagc aaacacaact ctggctacag cgcagcgaaa
     9661 tttggtggcg tcgggctgac tcaatcactg gcgctggatc tggcggagta cggcattacg
     9721 gtgcattcgc tgatgctcgg taacctgctg aaatcgccta tgttccagtc actgttgcca
     9781 caatacgcga ccaagctggg tatcaaaccg gagcaagtcg agcagtatta catcgataaa
     9841 gtaccgctca aacgcggctg cgattatcag gatgtgctga atatgctgct gttctacgcc
     9901 agtcctaagg cgtcgtactg cactggtcag tcgatcaatg tcaccggcgg tcaggtgatg
     9961 ttctgatcaa cagcggagat cttttaagga tctccgcgcg actaatagaa tgcctgatgc
    10021 gctacgctta tcaggcctac aggacttccg ccactacatt acggagaagt tatggtatcc
    10081 gcactcatca ccgtcgccgt tatcgcctgg tgtgcgcaac tggccttagg cggttggcaa
    10141 atttctcgtt ttaaccgtgc cttcgacacg ctatgccagc aagggagggt tggcgtgggc
    10201 cgttccagcg ggcgctttaa accgcgggtc gtggtcgcta ttgcgctgga cgatcagcag
    10261 cgcatcgtcg acaccttgtt tatgaaagga ctgaccgtct tcgcccgacc gcaaaaaatt
    10321 cccgcaatta ccggcatgca tgtgggtgat ttacagcccg atgtgatctt tccccatgat
    10381 ccactatcac agaatgctct atcattggcg cttaaactga aacgtggata atttcgttgt
    10441 gaatgttact tgcttgcgaa gttatcattt tgaaacctaa atcaggtaat cacgcccatg
    10501 aaacctcgtc agcgtcaggc cgccattctg gagtatctac aaaagcaggg taaatgctcg
    10561 gttgaagaat tggcgcaata ctttgacacc acaggcacaa ccattcgcaa agatctggtc
    10621 attctggaac atgccggaac cgtcattcgt acttatggcg gcgtggtgtt gaacaaagag
    10681 gaatccgatc cgcctatcga tcataaaacg cttatcaaca cccacaaaaa agagctgatt
    10741 gcagaagctg ccgttggttt tattcatgat ggcgactcaa taattcttga tgctggcagt
    10801 accgtgttgc agatggtgcc tctgctatcg cgctttaata acatcacggt gatgaccaac
    10861 agcctgcaca tcgtcaatgc gctatccgaa ctggataacg aacaaactat cctgatgcca
    10921 ggcggaacat ttcgcaaaaa atcggcctca tttcacgggc aactggcaga gaatgccttc
    10981 gagcatttca ccttcgataa attgtttatg ggcaccgacg gcatcgatct caatgcgggc
    11041 gtaaccacct ttaacgaggt ctataccgtc agtaaggcga tgtgtaatgc cgcgcgcgaa
    11101 gtgattttga tggctgactc atcgaagttt ggtcgtaaaa gccccaacgt ggtttgtagt
    11161 cttgaaagcg tcgataagct gattaccgac gcaggtatcg atccagcgtt tcgtcaggcg
    11221 ctggaagaaa aagggatcga cgtgatcata accggagaga gcaatgagtg aagcactact
    11281 gaacgcggga cgtcagacgt taatgctgga gttgcaggaa gcaagccgtt taccagaacg
    11341 tctgggcgat gattttgttc gcgccgccaa tatcatcctg cactgcgaag gcaaagtggt
    11401 ggtttcggga attggcaaat cgggccacat tggtaagaaa atcgccgcaa cgcttgccag
    11461 caccggcact cctgcctttt ttgtccatcc ggcagaggca ttgcacggcg acctggggat
    11521 gatcgaaagc cgcgatgtga tgctgtttat ctcttactcc ggtggcgcga aggaactgga
    11581 tctgattatt ccgcgtctgg aagataaatc tatcgcgctg ctggcgatga ccggcaaacc
    11641 gacgtcaccg ctgggcctgg cggcgaaagc ggtgctggat atctccgtag aacgcgaagc
    11701 ctgtccgatg caccttgcgc cgacctccag caccgtgaat accctgatga tgggcgacgc
    11761 gctggcgatg gcagtcatgc aggcgcgtgg ttttaatgaa gaagattttg cccgctccca
    11821 cccagccggg gcactgggcg ctcgcttgct gaacaaagtg catcatctta tgcgtcgtga
    11881 cgatgccatc ccgcaggtgg cgttaaccgc cagcgtgatg gacgcgatgc tggaacttag
    11941 ccgcaccggt ttaggtctgg tggcagtgtg cgatgatcaa cgactggtga aaggcgtctt
    12001 taccgacggt gatttacgtc gctggctggt tggcggcggc gcactcacca cgccagtcaa
    12061 tgaagcgatg acggtcggcg gcaccacgtt gcaatcgcaa agccgtgcca tcgacgccaa
    12121 agaaatcctg atgaaacgca aaatcaccgc cgcaccggta gtggatgaaa acggcaaact
    12181 caccggcgca attaacctgc aggatttcta tcaggccggg attatttaat ccttcagccc
    12241 cagacgtttc gccagccgat gcaggttggc gacgtcggtt tccagcatcc gcgcacaggc
    12301 ggcccagttg tgatgatttt gcgccagtgc ctgacgaatg gtttcacgct ggaacgcttc
    12361 tgtcgcttca cgcagatttt gcttaacaac gggcaccgcc gccgcttctg gcggcggcaa
    12421 cgtcacctca ggaaaagcaa aatgttgcgc ctcaagaatc acttcatcgc cgctgcgggt
    12481 ggctctcgac aatactaccg cccgatgaat agcatgttcc agttcgcgca cgtttcccgg
    12541 aaaattgtag tgttgcagta aatttcgcgc tccggcactt aataccacgc gggagagccc
    12601 cagccgcaaa cgacactgct cgcagaaata ccccgccagc agaatgacat catcgccccg
    12661 ctcacgcagc ggcggcactg aaagtggaaa cacgctcagg cggtggaaca agtcagcgcg
    12721 aaatcgccct gccagtacct cttcgcgtaa gtcacggtta gtcgccgcca gcacgcgcac
    12781 atcgacccgc aaactgcggt catcgccaac gcgttgaata tcgccatact gcaacaccct
    12841 cagcagcttg gcctgcaatg ccaacgacaa ctcgccgatc tcatcgagaa acagcgtgcc
    12901 gttatccgcc atttcaaact tcccgctgcg gttactgata gcaccagtaa acgccccttt
    12961 cacatgcccg aacaactcac tttccgccac actttccggc agtgcggcac agttgagata
    13021 gaccagcgga ttcaccgccc gtggcgaggc ttcatgaatc gctttcgcca ccagctcctt
    13081 accggttccc gtctcaccgc taattaaaac gttgagatcg gatgccgcca caatctcaat
    13141 ctcttttttc aattgcgtca tgcctggcga cagaccgatc atctgcgtct gtttcaccgc
    13201 ttcaaacggc gcggcttcgc ctggcagcat attctggctt tccagttgtt caatcagtaa
    13261 cgcattgctt aacgctcccg ccgccagcgc agcaatcagg cgtaactctt cgtcgctgaa
    13321 aacatcgaac tgatcgggct gcatcccgtc gagcgtcaag gcaccgatca ggttttgccc
    13381 ggcaaacaac ggcaaaccaa cgcaggcgtg aaccttcaga ctctcttgcc ctggaatcag
    13441 accgtcataa ggatcgggca attcgctgtc tgcgggaaag cgcaccacat cacccgcgcg
    13501 ggcaatcgct tccagccgtg gatgcccttc cagcgcaaag cgtctaccga gtacatcctt
    13561 cgccagcccg tcgatggcaa gcggaataaa ctgccgcgag tcgtaacgta gcaacgcaga
    13621 cgcatcgcac tccagcacct gacgcagcgt ggtgatcagg cgctgaaaac gatcctgatg
    13681 accaatccca cgctgcaatt cgatggcgat attcgccagc acatcaacgg aaaaactcat
    13741 ctttgcctca ctgtcaattt gactatagat attgtcatat cgaccatttt attgatag
//
LOCUS       DADSYM010000127        13794 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2415
            SAMN08039579-rid11723173.denovo.128, whole genome shotgun sequence.
ACCESSION   DADSYM010000127 DADSYM010000000
VERSION     DADSYM010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039579
            Sequence Read Archive: SRR6362533
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13794)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13794)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980162.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 03:39:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,484
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 4,347
            CDSs (with protein)               :: 4,347
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 43 of 85
            Pseudo Genes (incomplete)         :: 37 of 85
            Pseudo Genes (internal stop)      :: 19 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13794
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039579-rid11723173.denovo.128"
                     /strain="LGE2415"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8302 N 95.9628 W"
                     /collection_date="2015-08-24"
     gene            complement(353..778)
                     /gene="arsC"
                     /locus_tag="J1Y83_003365"
     CDS             complement(353..778)
                     /gene="arsC"
                     /locus_tag="J1Y83_003365"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-dependent arsenate reductase"
                     /protein_id="HBA7631481.1"
                     /translation="MSNITIYHNPACGTSRNTLEMIRNSGTEPTIIYYLETPPTRDEL
                     VKLIADMGITVRALLRKNVEPYEKLGLAEDKFTHDQLIDFMLQHPILINRPIVVTPLG
                     TRLCRPSEVVLEILPDAQKGAFTKEDGEKVVDEAGNRLK"
     gene            1312..1395
                     /locus_tag="J1Y83_003366"
     CDS             1312..1395
                     /locus_tag="J1Y83_003366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165328.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Damage inducible protein"
                     /protein_id="HBA7631482.1"
                     /translation="MIDKAIIVLGALIALLELIRFLLQLLN"
     gene            complement(1449..2801)
                     /gene="gorA"
                     /locus_tag="J1Y83_003367"
     CDS             complement(1449..2801)
                     /gene="gorA"
                     /locus_tag="J1Y83_003367"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003861215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HBA7631483.1"
                     /translation="MTKHYDYIAIGGGSGGIASINRAAMYGQKCALIEAKELGGTCVN
                     VGCVPKKVMWHAAQIREAIHMYGPDYGFDTTINKFNWETLIASRTAYIDRIHTSYENV
                     LGKNNVDVIKGFARFVDAKTIEVNGETITADHILIATGGRPSHPDIPGVEYGIDSDGF
                     FALPALPERVAVVGAGYIAVELAGVINGLGAKTHLFVRKHAPLRSFDPMISETLVEVM
                     NAEGPQLHTNAIPKAVVKNADGSLTLELEDGRSETVDCLIWAIGREPANDNINLEAAG
                     VKTNEKGYIVVDKYQNTNVEGIYAVGDNTGAVELTPVAVAAGRRLSERLFNNKPDEHL
                     DYSNIPTVVFSHPPIGTVGLTEPQAREQYGDDQVKVYKSSFTAMYTAVTTHRQPCRMK
                     LVCVGPEEKIVGIHGIGFGMDEMLQGFAVALKMGATKKDFDNTVAIHPTAAEEFVTMR
                     "
     gene            complement(2873..3715)
                     /locus_tag="J1Y83_003368"
     CDS             complement(2873..3715)
                     /locus_tag="J1Y83_003368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2030)-N(6))-methyltransferase
                     RlmJ"
                     /protein_id="HBA7631484.1"
                     /translation="MLSYRHSFHAGNHADVLKHTVQSLIIESLKEKDKPFLYLDTHAG
                     AGRYQLGSEHAERTGEYLEGIARIWQQDDLPAELEAYINVVKHFNRSGQLRYYPGSPL
                     IARQLLREQDSLQLTELHPSDYPLLRSEFQKDSRARVEKADGFQQLKAKLPPVSRRGL
                     ILIDPPYEMKTDYQAVVSGIAEGYKRFATGTYALWYPVVLRQQIKRMIHDLEATGIRK
                     ILQIELAVLPDSDRRGMTASGMIVINPPWKLEQQMNNVLPWLHSKLVPAGTGHATVSW
                     IVPE"
     gene            3918..5960
                     /gene="prlC"
                     /locus_tag="J1Y83_003369"
     CDS             3918..5960
                     /gene="prlC"
                     /locus_tag="J1Y83_003369"
                     /EC_number="3.4.24.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptidase A"
                     /protein_id="HBA7631485.1"
                     /translation="MTNPLLTPFELPPFSKILPEHVVPAVTKALNDCRENVERVVAQG
                     APYTWENLCQPLAEVDDVLGRIFSPVSHLNSVKNSPELREAYEQTLPLLSEYSTWVGQ
                     HEGLYKAYRDLRDGDHYATLNTAQKKAVDNALRDFELSGIGLPKEKQQRYGEIATRLS
                     ELGNQYSNNVLDATMGWTKLVTDEAELAGMPESALAAAKAQAEAKELEGYLLTLDIPS
                     YLPVMTYCDNQALREEMYRAYSTRASDQGPNAGKWDNSKVMEEILALRHELAQLLGFE
                     NYAFKSLATKMAENPQQVLDFLTDLAKRARPQGEKELAQLRAFAKAEFGVDELQPWDI
                     AYYSEKQKQHLYSISDEQLRPYFPENKAVNGLFEVVKRIYGITAKERKDVDVWHPDVR
                     FFELYDENDELRGSFYLDLYARENKRGGAWMDDCVGQMRKADGSLQKPVAYLTCNFNR
                     PVNGKPALFTHDEVITLFHEFGHGLHHMLTRIETAGVSGISGVPWDAVELPSQFMENW
                     CWEPEALAFISGHYETGEPLPKELLDKMLAAKNYQAALFILRQLEFGLFDFRLHAEFR
                     PDQGAKILETLAEIKKLVAVVPSPSWGRFPHAFSHIFAGGYAAGYYSYLWADVLAADA
                     FSRFEEEGIFNRETGQSFLDNILSRGGSEEPMELFKRFRGREPQLDAMLEHYGIKG"
     gene            5968..6720
                     /gene="rsmJ"
                     /locus_tag="J1Y83_003370"
     CDS             5968..6720
                     /gene="rsmJ"
                     /locus_tag="J1Y83_003370"
                     /EC_number="2.1.1.242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709277.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(1516)-N(2))-methyltransferase
                     RsmJ"
                     /protein_id="HBA7631486.1"
                     /translation="MKICLIDETGAGDGALSVLAARWGLEHDEDNLMALVLTPEHLEL
                     RKRDEPKLGGIFVDFVGGAMAHRRKFGGGRGEAVAKAVGIKGDYLPDVVDATAGLGRD
                     AFVLASVGCRVRMLERNPVVAALLDDGLARGYADAEIGGWLQERLQLIHASSLTALSD
                     ITPRPQVVYLDPMFPHKQKSALVKKEMRVFQSLVGPDLDADGLLEPARLLATKRVVVK
                     RPDYAPPLANVATPNAVVTKGHRFDIYAGTPV"
     gene            complement(6769..8238)
                     /gene="dtpB"
                     /locus_tag="J1Y83_003371"
     CDS             complement(6769..8238)
                     /gene="dtpB"
                     /locus_tag="J1Y83_003371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpB"
                     /protein_id="HBA7631487.1"
                     /translation="MNTTTPMGMLQQPRPFFMIFFVELWERFGYYGVQGVLAVFFVKQ
                     LGFSQEQAFVTFGAFAALVYGLISIGGYVGDHLLGTKRTIVLGALVLAIGYFMTGMSL
                     LKPDLIFIALGTIAVGNGLFKANPASLLSKCYPPKDPRLDGAFTLFYMSINIGSLIAL
                     SLAPVIADKFGYSVTYNLCGAGLIIALLVYIACRGMVKDIGSEPDFRPMSFSKLLYVL
                     LGSVVMIFVCAWLMHNVEVANLVLIVLSIIVTIIFFRQAFKLDKTGRNKMFVAFVLML
                     EAVVFYILYAQMPTSLNFFAINNVHHEILGFSINPVSFQALNPFWVVLASPILAGIYT
                     HLGNKGKDLSMPMKFTLGMFMCSLGFLTAAAAGMWFADAQGLTSPWFIVLVYLFQSLG
                     ELFISALGLAMIAALVPQHLMGFILGMWFLTQAAAFLLGGYVATFTAVPDNITDPLET
                     LPVYTNVFGKIGLVTLGVAVVMLLMVPWLKRMIATPESH"
     gene            complement(8555..8989)
                     /gene="uspA"
                     /locus_tag="J1Y83_003372"
     CDS             complement(8555..8989)
                     /gene="uspA"
                     /locus_tag="J1Y83_003372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspA"
                     /protein_id="HBA7631488.1"
                     /translation="MAYKHILIAVDLSPESKVLVEKAVSMARPYNAKVSLIHVDVNYS
                     DLYTGLIDVNLGDMQKRISEETHHALTELSTNAGYPITETLSGSGDLGQVLVDAIKKY
                     DMDLVVCGHHQDFWSKLMSSARQLINTVHVDMLIVPLRDEEE"
     gene            9380..9715
                     /gene="uspB"
                     /locus_tag="J1Y83_003373"
     CDS             9380..9715
                     /gene="uspB"
                     /locus_tag="J1Y83_003373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspB"
                     /protein_id="HBA7631489.1"
                     /translation="MISTVALFWALCVVCIVNMARYFSSLRALLVVLRNCDPLLYQYV
                     DGGGFFTSHGQPNKQVRLVWYIYAQRYRDHHDDEFIRRCERVRRQFILTSALCGLVVV
                     SLIALMIWH"
     gene            complement(9786..11285)
                     /gene="pitA"
                     /locus_tag="J1Y83_003374"
     CDS             complement(9786..11285)
                     /gene="pitA"
                     /locus_tag="J1Y83_003374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter PitA"
                     /protein_id="HBA7631490.1"
                     /translation="MLHLFAGLDLHTGLLLLLALAFVLFYEAINGFHDTANAVATVIY
                     TRAMRSQLAVVMAAVFNFLGVLLGGLSVAYAIVHMLPTDLLLNMGSSHGLAMVFSMLL
                     AAIIWNLGTWYFGLPASSSHTLIGAIIGIGLTNALMTGTSVVDALNIPKVLSIFGSLI
                     VSPIVGLVFAGGLIFLLRRYWSGTKKRARIHLTPAEREKKDGKKKPPFWTRIALILSA
                     IGVAFSHGANDGQKGIGLVMLVLIGVAPAGFVVNMNATGYEITRTRDAINNVEAYFEQ
                     HPALLKQATGADQLVPAPEAGATQPAEFHCHPSNTINALNRLKGMLTTDVESYDKLSL
                     DQRSQMRRIMLCVSDTIDKVVKMPGVSADDQRLLKKLKSDMLSTIEYAPVWIIMAVAL
                     ALGIGTMIGWRRVATTIGEKIGKKGMTYAQGMSAQMTAAVSIGLASYTGMPVSTTHVL
                     SSSVAGTMVVDGGGLQRKTVTSILMAWVFTLPAAVLLSGGLYWLSLQFL"
     gene            11517..12710
                     /locus_tag="J1Y83_003375"
     CDS             11517..12710
                     /locus_tag="J1Y83_003375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001683372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HBA7631491.1"
                     /translation="MERFDAIIIGAGAAGMFCSALAGQAGRRVLLIDNGKKPGRKILM
                     SGGGRCNFTNLYVEPGAYLSQNPHFCKSALARFTQWDFIDLVNKHGIAWHEKTLGQLF
                     CDDSAQQIVDMLVDECEKGNVAFRLRSEVLSVAKDDTGFTLELNGMTVGCEKLVIATG
                     GLSMPGLGASPFGYKIAEQFGLNVLPTRAGLVPFTLHKPLLEELQVLAGVAVPSVITA
                     ENGIVFRENLLFTHRGLSGPAVLQISSYWQPGEFVSINLLPDVDLETFLNEQRNAHPN
                     QSLKNTLAVHLPKRLVERLQQLGQIPDVSLKQLNVRDQQILISTLTDWRVQPNGTEGY
                     RTAEVTLGGVDTNELSSRTMEARKVPGLYFIGEVMDVTGWLGGYNFQWAWSSAWACAQ
                     DLVAE"
     gene            complement(12797..>13794)
                     /locus_tag="J1Y83_003376"
     CDS             complement(12797..>13794)
                     /locus_tag="J1Y83_003376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417948.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2776 domain-containing protein"
                     /protein_id="HBA7631492.1"
                     /translation="CIALGEFVLTGSGQSEYFVAGHVLISLSAICLALFTTAFIIISQ
                     LTHGMNKFYNRLFPVIGYAGSATTMIWGWSLLASNNVMADEFVAGHVIFGVGMIAACV
                     STVAASSGHFLLIPKNASGSKSDGTPLQAYSSTIGNCLIAVPVLLTLFGFIWSVILLR
                     SADITPHYVAGHVLMGLTAICACLIGLVATIVHQTRNTFSVKEHWLWCYWVILLGSLT
                     IIFGIYVLISSDASARLAPGIILICLGMICYSIFSKVWLLALVWRRTCSLANRIPMIP
                     VFTCLFCLFLAAFLAEIAQTDMAYFIPSRVLVGLGAVCFTLFSIVSILEAGSAKK"
BASE COUNT         3516 a         3480 c         3631 g         3167 t
ORIGIN      
        1 ttttgtgatg ttttagatat gtaataataa aatgggttaa cgtatgttga aattatttat
       61 tgtgtaaacc actgatagat cataatccag tacatcttta cagtgctgca ctgtatcgct
      121 gataactaag cagctcactg atacaccatg ctggacgcga taattggaat ctcacgcagg
      181 gagtactgtg taaatagcaa aattgcctga tgcgcttcga ctgtcaggcc tacaaataac
      241 atacaattca tcgaattatc ataacgttgt aggtcggata agacgcgcca gcgtcgcatc
      301 cgacataaat aaagcgcatt tttctaacaa cctgtggggg gatatagccg ctttatttca
      361 ggcgattacc cgcctcatca accactttct cgccatcttc cttggtgaac gcgccttttt
      421 gcgcatctgg cagaatttcc agcacgactt ctgaagggcg gcacaggcga gttcccagcg
      481 gcgtcaccac aatcgggcga ttaataagaa tcgggtgctg aagcataaag tcgattaact
      541 gatcgtgagt aaatttatct tctgcaaggc ccagtttctc atacggctcg acgtttttac
      601 gcagcaacgc gcgtacggta atccccatat cggcaatgag ttttaccagc tcatcgcgcg
      661 tcggtggtgt ttccagataa tagataatgg tcggttctgt accgctgttg cggatcatct
      721 ccagcgtatt acgcgacgta ccgcaggccg ggttgtgata aatggtaatg ttgctcatat
      781 cagtatctca ttacgatgtg aaagagaggt gtagcgccag cgccaccaga gttacaaacc
      841 aaagcggaca agtcaaaaac acatatggtt taacgaatat gtaagtgtag agaaaggtgt
      901 aggtgtgaat taagcgttgg gcattcattt gaaaggaggt ctgaatcagg acgtaaaaaa
      961 accgacttcg cgtcggtttt tttactgttc agccctgagt tggtggctct ggctggagtg
     1021 agatagccat catctaacgc atcaatgcta ataccatact gaaactattg caaggacatg
     1081 ctggttttat aacccgcaag tactgtatga ttatccagtt agctctgaga cattttcact
     1141 ctggcaatgc gcataaacgc tttcaaagtc ctggtctgaa gtacgggtgg tgccgttaac
     1201 tgatgctctg gccggagtga gatagtcgtc atctaacaat gagacatgcg ccgtgacagg
     1261 caatggatga gtaagcggat gcattctcac tccatcgcat ggagaaaacg ggtgattgat
     1321 aaagcaatca tcgttcttgg ggcgttaatt gcgctgctgg aactgatccg ctttctgctt
     1381 cagcttctga actgatagcg gaaacgtagc taagggccaa gagcacacta ctcttagccc
     1441 tttaacattt aacgcattgt cacgaactct tctgccgctg tcgggtgaat ggcaaccgtg
     1501 ttgtcgaagt cttttttggt tgcacccatc ttcagcgcca ccgcgaagcc ctgcaacatt
     1561 tcgtccatac caaaaccaat gccgtgaata ccgacaatct tctcttccgg tccaacgcat
     1621 accagtttca tgcggcacgg ctggcggtga gtggtgacgg cggtatacat cgcggtgaaa
     1681 gaggatttat acactttcac ctgatcgtcg ccgtactgct cgcgcgcctg cggctccgtt
     1741 aaaccaacag taccaatcgg cggatggctg aagaccacgg tcggaatgtt gctgtaatcc
     1801 agatgctcat ccggcttgtt attaaacagg cgttcagaga gacggcgacc tgctgcaaca
     1861 gccaccggtg tcagttccac tgcacccgtg ttatcgccca ccgcgtaaat accttcaacg
     1921 ttggtgtttt gatatttatc gacgacgata taaccttttt cgttagtttt aacgccagcg
     1981 gcttccaggt tgatgttgtc attggcaggc tcgcgaccaa tcgcccaaat caggcaatcc
     2041 accgtttcac tgcgaccatc ttccagctcc agcgtcaggc taccatcggc atttttcact
     2101 accgctttcg ggatggcgtt ggtgtgcaac tgcgggcctt cggcgttcat cacttcaacc
     2161 agcgtttcag agatcatcgg gtcgaagctg cgcagcggcg catgtttacg cacaaacaga
     2221 tgcgtcttcg caccgaggcc gttaatcacg cccgccagtt caacggcgat gtaacccgcg
     2281 ccaacaaccg ctacacgctc tggcaaagcc ggaagggcga agaagccatc agaatcaata
     2341 ccgtattcca cgcctggaat atccgggtgg ctcggacgac caccggtggc gatcagaata
     2401 tgatcggccg tgatggtttc gccgttgact tcaatggttt tggcatcaac gaagcgggca
     2461 aagcctttga ttacatcaac gttattttta ccgagcacgt tctcatagga agtatgaata
     2521 cggtcgatat aggcggtacg gctggcgatc aacgtttccc agttgaattt attgatagtg
     2581 gtatcaaaac cataatccgg gccgtacata tggatcgctt cacggatttg cgccgcgtgc
     2641 cacatcactt ttttcggcac acagccaaca tttacgcagg tgccgcccag ctctttggct
     2701 tcaatcagtg cgcatttctg gccgtacata gccgcgcggt tgatggaggc gataccgccg
     2761 ctgccgccgc cgatggcgat gtaatcatag tgtttagtca tgacatattg tccttatcgt
     2821 tgattaccgc gattgtagcg cgagacgtaa taggtgccag caatggctgc aattactctg
     2881 gcacgatcca gcttacggtg gcgtgcccgg tgcctgccgg aaccagtttg ctgtgcagcc
     2941 acggcagcac gttattcatc tgctgttcca gcttccacgg cgggttaatc acaatcatgc
     3001 cagaagcggt catgccacgg cgatcgctgt ctggcagcac cgccagttca atttgcagaa
     3061 ttttgcgaat gccggtcgct tccaggtcgt ggatcatgcg cttaatttgc tggcgcagca
     3121 ccactggata ccacaatgcg taagtgccag tggcgaaacg tttgtaacct tctgctatcc
     3181 cgctgaccac cgcctgatag tcggttttca tttcatacgg cgggtcgata aggattaaac
     3241 cacggcggga aaccggcggc agcttggcct taagctgctg gaaaccgtcg gctttttcga
     3301 cgcgcgcacg gctgtctttc tgaaattcag aacgcagcaa cgggtaatcg ctcgggtgca
     3361 gttcggtcag ttgcaggctg tcctgttcac gcagtagctg gcgagcaatc aacggcgaac
     3421 cggggtaata acgcaactgc ccgctacggt tgaagtgttt taccacattg atgtacgcct
     3481 ccagctccgc aggcaaatcg tcctgctgcc agatacgggc gatgccttcg agatattcgc
     3541 cggtacgctc ggcatgttcg ctgcctaact gataacgccc ggcccctgcg tgggtgtcga
     3601 gatagagaaa cggtttatct ttctctttca gcgactcaat gatcaggctc tgaacggtat
     3661 gtttaaggac gtcggcgtgg ttgccagcgt gaaagctgtg gcgataactg agcatgggta
     3721 aaggtgttcc ggtaagtaaa aatcggcccg tattcggggc gcacaaaagc ctatcaggac
     3781 agtataccga aaagaggccg ccgccgcgaa agcgtaacgt ttctcattga aattcactac
     3841 acttaacccc atgctacaaa cattatgtaa agcgcctgtt gagcgcttcc ttaacctctt
     3901 taaccaggac tgcgctaatg acgaatccgt tactgactcc ctttgaattg cctccgtttt
     3961 ctaaaattct cccggaacat gtcgttccag ccgtgactaa ggcgctgaac gactgccgcg
     4021 aaaatgtgga gcgcgtagta gcgcaagggg caccgtacac ctgggaaaat ctctgccagc
     4081 cgttggcgga agtggacgat gtgctggggc gtatcttctc cccggtcagc catctgaact
     4141 cggtgaaaaa tagcccggaa ctgcgtgaag cgtacgaaca aaccctgccg ctgctgtcag
     4201 aatacagcac ctgggtaggg caacatgaag ggctgtataa ggcatatcgc gacctgcgcg
     4261 atggcgatca ttacgccacg ctgaacacgg cgcagaaaaa agcggttgat aacgcactgc
     4321 gcgacttcga actctctggc attggtctgc cgaaagagaa acagcagcgt tacggcgaaa
     4381 ttgctacccg tctttctgaa ctgggcaacc agtacagcaa caacgtcctc gatgcgacga
     4441 tgggctggac caaactcgtt accgacgaag cggagctggc ggggatgcca gaaagcgcgc
     4501 tggctgcggc aaaagcccag gctgaagcga aagagctgga aggttatttg ctgacgctgg
     4561 atatcccaag ctacctgccg gtaatgacct actgcgacaa ccaggctctg cgcgaagaga
     4621 tgtatcgcgc ttatagcact cgtgcttccg atcaaggtcc gaacgccggt aagtgggata
     4681 acagcaaggt gatggaagag atcctcgcgc tgcgtcacga actggcgcaa ctgctgggct
     4741 ttgaaaacta cgccttcaaa tcacttgcca ctaaaatggc agaaaacccg cagcaggtgc
     4801 tggatttctt aaccgatctg gcaaaacgcg cgcgtccgca aggcgaaaaa gagctggcgc
     4861 aactgcgcgc cttcgccaaa gccgaatttg gcgtcgatga gttacaaccg tgggatattg
     4921 cttactacag cgaaaaacag aaacagcacc tctacagcat cagcgatgaa caactgcgtc
     4981 cgtacttccc ggaaaacaaa gcggttaacg gcctgtttga agtggtgaaa cgtatttacg
     5041 gcatcaccgc taaagagcgt aaagatgttg atgtctggca tccggatgta cgtttcttcg
     5101 agctgtatga cgagaacgac gaactgcgcg gcagcttcta cctcgacctg tatgcccgtg
     5161 aaaacaaacg cggcggggcg tggatggatg actgcgtagg ccagatgcgt aaagccgacg
     5221 gttcgctgca aaaaccggtc gcgtatctga cctgcaactt caaccgcccg gtaaatggta
     5281 aaccggcgct gtttacccat gacgaagtga tcaccctgtt ccacgagttc ggtcacggcc
     5341 tgcaccatat gctgacccgc attgaaaccg ctggtgtttc cgggatcagt ggggtgccgt
     5401 gggatgcggt cgaactgccg agccagttta tggaaaactg gtgctgggag ccggaggcgc
     5461 tggcgtttat ctccggtcac tatgaaaccg gcgagccgct gccgaaggag ctgctggata
     5521 aaatgctggc agcgaaaaat taccaggcgg cgctgtttat tctgcgccag ctggagttcg
     5581 gcctgttcga tttccgcctc catgccgagt tccgtccgga tcagggggcg aaaatcctcg
     5641 aaactctggc agaaatcaag aaactggttg ccgtggtacc gtctccgtca tggggccgtt
     5701 tcccgcatgc tttcagccac atttttgccg gtggttatgc ggcaggttac tacagctacc
     5761 tgtgggccga tgtgctggcg gcagatgcct tctcgcgttt tgaggaagag ggcattttca
     5821 accgtgaaac tgggcagtcg ttcctcgaca acattctgag ccgtggcggt tcagaagagc
     5881 cgatggagct gttcaaacgc ttccgtggtc gtgaaccaca gctggatgcg atgctggagc
     5941 attacggcat taagggctga tcattcagtg aaaatctgct taattgatga aacaggcgcc
     6001 ggagacggcg ccttatctgt tctggcggcc cgctgggggc tggaacacga tgaagacaac
     6061 ctgatggcgc tggtgttaac gccggagcat ctggaactgc gcaagcgtga tgagccgaaa
     6121 cttggcggca tctttgttga ttttgttggc ggagcgatgg cgcaccgacg caaattcggc
     6181 ggcggacgtg gtgaagcggt ggcaaaagca gtgggcatta aaggcgatta tttgccggat
     6241 gtggtggacg ccaccgcagg attagggcgc gatgcctttg tgctggcttc ggtcggctgc
     6301 cgcgtgcgga tgctggagcg taatccggtg gttgccgcgt tgctcgacga tggtctggcg
     6361 cgtggttatg cggatgcgga aatcggcggc tggttacagg aacgtttgca gttgattcac
     6421 gcctccagtc tgacggcgct gagcgatatc accccgcgtc cgcaggtagt ttatcttgac
     6481 ccaatgttcc cgcataagca gaaaagcgcg ctggtgaaaa aagagatgcg cgtgtttcag
     6541 tcgctggtgg ggccggatct tgatgccgat ggattgttgg agcctgcgcg gttgctggca
     6601 accaaacgcg tggtggtgaa gcgtccggac tatgcgccgc cgctggcgaa tgtcgccacg
     6661 ccaaacgcgg tagtcactaa agggcatcgc tttgatattt atgccggaac gccggtgtaa
     6721 aagtaaagca gggtagcgtt atcgctaccc tgctttctgc aagaataatt aatggctttc
     6781 cggcgtcgca atcatgcgtt tcagccacgg caccatcaat agcatcacta cggcaacgcc
     6841 cagcgtaacc aggccaatct taccaaacac gttggtatag acgggcaacg tctcaagtgg
     6901 atcggtaatg ttgtctggta ctgcggtaaa tgttgccaca tagccgccca gcaagaacgc
     6961 ggcagcctgc gtcaggaacc acatcccgag aataaagccc atcaaatgct gcggcaccag
     7021 ggcagcaatc atcgccaggc caagggcgct aataaacagt tcacctaagc tctggaataa
     7081 gtacaccagc acgataaacc atggcgatgt cagcccctgg gcatccgcaa accacattcc
     7141 agccgctgca gccgtcaaaa agcccagtga gcacataaac atgccgagag taaacttcat
     7201 tggcatcgag agatctttgc ctttgttacc cagatgcgtg taaatgcctg ccagtattgg
     7261 gctggcgagt accacccaga acgggttaag cgcctggaag ctgaccgggt tgatggaaaa
     7321 gccgagaatt tcatgatgca cgttgttgat ggcaaagaag ttcagcgacg ttggcatctg
     7381 ggcgtagaga atgtaaaaca ccaccgcttc gagcatcagg acaaaggcga caaacatttt
     7441 attgcgcccg gttttatcca gcttgaatgc ctgacgaaag aagatgatgg tgacgatgat
     7501 ggagagaaca atcagcacca gattggcgac ttctacgttg tgcatcagcc aggcgcagac
     7561 gaagatcatc accacgctgc caagtaatac gtacaacagt ttactgaagc tcatcggccg
     7621 gaagtcgggt tcagaaccaa tgtctttcac cattccacga caggcgatgt aaaccagtaa
     7681 tgcgataatt aaccctgcac cgcacaggtt gtaggtgact gaatagccga atttatcagc
     7741 gatcacaggg gccagcgata acgctatcaa cgagccgata ttgatcgaca tatagaacag
     7801 ggtgaatgcg ccatcaagcc gcggatcttt cggcgggtag cacttcgaaa gcaagctggc
     7861 tgggttagct ttaaacaggc cgttaccgac agcgatagtc cccagggcga tgaaaatcag
     7921 gtcaggctta agtagcgaca tgccggtcat gaagtagcca atcgccagca caagtgctcc
     7981 gagaacaatg gtgcgtttgg ttcccagcag gtggtcgcca acatagccac caatggaaat
     8041 gaggccatag accagcgcag caaaagcacc aaaagtgaca aaagcctgct cttgcgagaa
     8101 cccaagctgt ttaacgaaga aaaccgccag tacgccctgc acgccgtagt agccgaatcg
     8161 ctcccataac tcgacaaaaa agatcatgaa aaatgggcga ggttgctgca gcatccccat
     8221 gggtgttgtt gtattcatat ttattaacct tccaatacca tcctgaaaag tatgaacgcg
     8281 taagggataa cgcgcgaaaa agtttacatc tgtcagagat tcttaatatt gatgcggcga
     8341 atagattaat acactattac aacagaaaat aaccagctga tcacactaat gatagaaaat
     8401 atgatggtgt taaatgttgt attggcaggg gttttgattg agtacgcaga ccgcaggcca
     8461 gataaggcgt ttacgctgca tcaggtaaca aggcgcgcca aaaagcaaaa cgcccgcata
     8521 tgcgggcgtt caggaacacg tcgtggaggg aagattattc ttcttcgtcg cgcagcggaa
     8581 caatcagcat atcaacgtga acggtgttga tcagctgacg tgcggaagac atcagtttgc
     8641 tccagaagtc ctggtggtga ccacaaacca ccagatccat atcgtatttc ttgattgcat
     8701 cgaccagtac ctggcccaga tcgccgctgc cgctcagggt ttcagtgatt gggtagcctg
     8761 cattggttga aagctcggtc agcgcatgat gtgtctcttc agagatgcgt ttctgcatat
     8821 cacccagatt cacatcgata agcccggtgt ataggtcaga gtagtttaca tctacgtgga
     8881 tcagagaaac tttcgcattg taggggcgag ccatagagac tgctttctct accagaactt
     8941 tgctttccgg ggagaggtcg accgcgatga gaatgtgttt ataagccata gtgttactcc
     9001 ttccataaag ttgtcgatga ctggccagct agcgtttctt gtgccgttac gtcacccgcg
     9061 tcctgcgaac tacgtgcgcg gggctaaccc agccgtaaag attcagtgtt aagaccatcc
     9121 ttaccttata gcgacccgga tgatccgtca atccgccttg cttactcgtt agataaacaa
     9181 attttcgaca tgaatatatt gatagtggtt aaccttctgg aaaaaaaaca acctgatctc
     9241 ctacactatc tatagagccg ctcggatgtt ggtcaccaca aaagcggttt ctccaggacc
     9301 tggtgttaac tgattggcag cgtatcggga gcggtagccc cggggtggaa ctccgcgggc
     9361 aggtcgccgg ggaggaggta tgataagcac cgtcgcatta ttttgggctt tatgtgtcgt
     9421 ttgcattgtt aacatggcgc gctatttctc atcactacgc gcgttgttag tggtactgcg
     9481 taactgcgat ccattgctct atcaatatgt tgatggaggg ggctttttta cctcacatgg
     9541 ccaacccaac aaacaggtgc gtctcgtttg gtatatctat gcccaacgtt atcgcgatca
     9601 tcacgatgat gagtttattc gccgctgtga gcgggtgcgt cggcagttta ttctgaccag
     9661 cgcattgtgt ggtctggtgg tggtcagcct gattgcattg atgatttggc attaagcact
     9721 tcagcaataa tgacgcataa aaaaagcggg ccagtaaact gacccgctcg ttttggtgcg
     9781 tacgattaca ggaactgcaa ggagagccag tacagcccgc cggaaagcag taccgcagcc
     9841 ggaagggtaa acacccaggc catcagaata ctggttacgg ttttacgctg taagccgccg
     9901 ccatctacca ccatcgtccc cgcgacagaa gaggagagta cgtgagtagt ggaaaccggc
     9961 atcccggtat aacttgccag gccgatagac actgccgccg tcatctgggc agacatcccc
    10021 tgagcgtagg tcatgccttt cttaccgatt ttctcaccga tagtcgttgc cacacgacgc
    10081 cagccaatca tcgtaccgat acctaacgcc agcgcgaccg ccatgatgat ccacaccggc
    10141 gcatactcga tggtgctaag catgtcggac ttcagtttct tcaacaggcg ctgatcgtca
    10201 gcactcacac caggcatctt caccactttg tcgatagtgt cagaaacgca cagcataatg
    10261 cggcgcatct ggctacgttg atcaagcgac agcttgtcgt agctttccac atcggtagtc
    10321 aacatgcctt tcaggcggtt gagcgcgtta atggtattcg acggatggca atggaactcc
    10381 gcgggttgcg ttgcgccagc ttccggagcc ggtactaact gatcagcacc ggtcgcttgt
    10441 ttcagcagtg caggatgctg ctcaaagtaa gcttcgacgt tgttgatggc atcacgggta
    10501 cgggtgattt cgtagccagt ggcattcatg ttcaccacga agcctgctgg tgcgacgcca
    10561 atcaatacca acataaccag accaatgcct ttctgaccat cgttcgcgcc gtgcgaaaac
    10621 gccacgccga tagcggaaag gatcagcgca atacgcgtcc agaacggcgg ctttttcttg
    10681 ccgtctttct tttcacgctc cgctggggtc aggtggatac gggcgcgttt cttggtgccg
    10741 ctccagtagc gacgcagcaa gaaaatcaga ccgccagcaa acaccaggcc gacaataggg
    10801 gaaacgatca gggaaccgaa aatacttaat actttcggga tattgagtgc atccaccact
    10861 gacgtcccgg tcatcaacgc attggttaaa ccaatcccga tgatcgcgcc aatcagcgta
    10921 tgagagctgg atgcaggtaa accaaagtac caggtaccca ggttccagat aatcgccgcc
    10981 agcaacatag agaacaccat ggcaaggcca tgagacgatc ccatattaag cagcagatcc
    11041 gtcggcagca tatgcacaat ggcataggca acactcagac cacccagcaa aacacccaaa
    11101 aagttgaaca ccgccgccat aaccacggcg agctgagaac gcatcgcgcg ggtatagata
    11161 acggttgcca cggcgttggc tgtgtcatgg aaaccattga tggcttcgta gaacagcaca
    11221 aaagccagtg caagcaataa taacagcccg gtatgcaaat ccaggccagc aaacaaatgt
    11281 agcataggac gttacgccat tttgaggaca tgaacgcggc gcattatcag tgactttcgc
    11341 ggcgcgggca aagtgaaata tagacttttt ttgatttgcc tcctgtatgg attacactca
    11401 aaaaataatt atcttatata attcaggcaa atacttcctt ttagtaatat tgatgctggt
    11461 gcgaccactg aggaatcttt acaattcacg cccgtttttt cttagaggag cgcaacgtgg
    11521 aaaggtttga tgccattatt ataggcgctg gtgcggcagg tatgttctgt tctgcgctgg
    11581 caggtcaggc aggacgccgg gttctgctga tcgataatgg taaaaaacca gggcgcaaaa
    11641 tcctcatgtc tggcggtggg cgctgcaact ttaccaacct ttatgtcgaa ccaggcgctt
    11701 atctgagcca gaatccgcat ttttgtaagt ctgcactcgc gcgttttacc cagtgggatt
    11761 tcattgatct ggtcaataaa cacggcatcg cctggcacga gaaaacgtta gggcagctct
    11821 tctgcgatga ctccgcgcag cagattgtcg acatgctggt ggatgagtgc gagaagggca
    11881 atgtggcctt cagattgcgt agcgaagtgc tgagcgtggc gaaggatgat acaggcttca
    11941 cgcttgaact gaacggcatg actgtcggtt gcgaaaagct ggtcattgcg accggtggac
    12001 tgtcaatgcc ggggctgggt gcatcgccgt ttggttataa gattgccgaa caattcggcc
    12061 tcaacgtgct gccgacccgt gcgggtctgg tgccattcac tctgcataaa ccgttgctcg
    12121 aggagttaca ggtgctggcg ggcgtggcgg tgccttccgt gattaccgct gaaaacggta
    12181 tcgttttccg tgagaactta ctcttcaccc accgcggctt atctggaccg gcggtgttgc
    12241 agatttccag ctactggcaa ccgggtgaat ttgtcagcat caatctgcta ccggatgtgg
    12301 acctcgaaac cttcctgaat gagcagcgta atgcacatcc gaatcaaagc ctgaaaaaca
    12361 cgctggcggt tcatctgccg aagcggttgg ttgaacggtt acaacaactc gggcaaatcc
    12421 cggatgtctc gctaaaacag ctcaacgtgc gtgaccaaca gatactgatt agcacattga
    12481 ctgactggcg cgtacaaccc aacggcactg aaggctatcg cactgccgaa gtgacgctcg
    12541 gcggcgtgga caccaacgaa ctctcttcac ggacgatgga agcgcgcaaa gtgcctgggc
    12601 tgtacttcat cggcgaagtg atggacgtca ccggctggct ggggggctat aacttccagt
    12661 gggcgtggtc gagtgcatgg gcttgtgcgc aggatttggt ggcagaataa gaagcaaaat
    12721 gcgctctcga taccagaggg attaacctga ataaaaccag ataaggcgtc caacgcctta
    12781 tctgaagcct tttttattat tttttcgctg aacccgcttc taatatagaa acgatggaaa
    12841 acagtgtaaa acaaactgca cctaagccaa ccagaactcg cgaaggaata aagtaagcca
    12901 tatcggtttg cgcaatttca gccaggaacg cagcaaggaa cagacaaaac agacaggtaa
    12961 ataccggaat catgggtatt ctgttggcta acgaacaggt gcgtcgccat accagcgcta
    13021 acaaccacac tttggagaaa atactgtagc agatcatccc caggcaaatg agaataatgc
    13081 cgggggccag gcgggcgctt gcatctgaac tgattaatac gtagatacca aaaataatgg
    13141 taagtgagcc aagcaaaata acccaatagc accacagcca atgttctttc actgagaacg
    13201 tattccgcgt ttgatggaca atagtggcga caaggccaat aagacaagcg caaattgccg
    13261 ttaaccccat tagtacgtga cctgcaacat aatgtggtgt gatatctgca ctacgtaaca
    13321 gtatgactga ccaaatgaaa ccaaagagag taagcagaac gggaacggca attaagcaat
    13381 taccgattgt tgaagaatag gcctgcaatg gcgtaccgtc actcttactg cccgaggcgt
    13441 ttttggggat caacagaaaa tgtccggaag atgccgctac ggttgataca caagcggcaa
    13501 tcatgccaac gccgaaaata acatgaccgg cgacaaattc atctgccatc acattattgc
    13561 ttgccaacag tgaccatccc caaatcatag ttgtagcaga accagcataa ccaataacag
    13621 gaaataacct gttgtagaat ttattcatgc cgtgagttaa ctgagatata ataataaatg
    13681 cggtagtgaa caatgccaga catatcgcag aaagagaaat cagaacatgc cctgcaacga
    13741 aatattcact ttgcccagaa ccggttaata caaactcacc taatgcgatg cata
//
LOCUS       DADSYM010000128        13791 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2415
            SAMN08039579-rid11723173.denovo.129, whole genome shotgun sequence.
ACCESSION   DADSYM010000128 DADSYM010000000
VERSION     DADSYM010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039579
            Sequence Read Archive: SRR6362533
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13791)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13791)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980162.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 03:39:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,484
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 4,347
            CDSs (with protein)               :: 4,347
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 43 of 85
            Pseudo Genes (incomplete)         :: 37 of 85
            Pseudo Genes (internal stop)      :: 19 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13791
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039579-rid11723173.denovo.129"
                     /strain="LGE2415"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8302 N 95.9628 W"
                     /collection_date="2015-08-24"
     gene            <1..916
                     /locus_tag="J1Y83_003377"
     CDS             <1..916
                     /locus_tag="J1Y83_003377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308823.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBA7631493.1"
                     /translation="LAESSSFNNAAKLLNITQPALTRRIKKMEEDLHIQLFERTTRKV
                     TLTKAGKRLLPEARELIKKFDETLFNIRDMNAYHRGMVTLACIPTAVFYFLPLAIGKF
                     NELYPNIKVRILEQGTNNCMESVLCNESDFGINMNNVTNSSIDFTPLVNEPFVLACRR
                     DHPLAKKQLVEWQELVGYKMIGVRSSSGNRLLIEQQLADKPWKLDWFYEVRHLSTSLG
                     LVEAGLGISALPGLAMPHAPYSSIIGIPLVEPVIRRTLGIIRRKDAVLSPAAERFFAL
                     LINLWTDDKDNLWTNIVQRQRHALQEIG"
     gene            complement(957..1952)
                     /gene="pgl"
                     /locus_tag="J1Y83_003378"
     CDS             complement(957..1952)
                     /gene="pgl"
                     /locus_tag="J1Y83_003378"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HBA7631494.1"
                     /translation="MKQTVYIASPESQQIHVWNLNHEGALTLTQVVDVPGQVQPMVVS
                     PDKRYLYVGVRPEFRVLAYRIAPDDGALTFAAESALPGSPTHISTDHLGQFVFVGSYN
                     AGNVSVTRLEDGLPVGVVDVVEGLDGCHSANISPDNRTLWVPALKQDRICLFTVSDDG
                     HLVAQDPAEVTTVEGAGPRHMVFHPNEQYAYCVNELNSSVDVWELKDPHGNIECVQTL
                     DMMPENFSDTRWAADIHITPDGRHLYACDRTASLITVFSVSEDGSVLSKEGFQPTETQ
                     PRGFNVDHSGKYLIAAGQKSHHISVYEIVGEQGLLHEKGRYAVGQGPMWVVVNAH"
     gene            2107..2925
                     /locus_tag="J1Y83_003379"
     CDS             2107..2925
                     /locus_tag="J1Y83_003379"
                     /EC_number="3.1.3.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphatase"
                     /protein_id="HBA7631495.1"
                     /translation="MTTRVIALDLDGTLLTPKKTLLPSSIEALARAREAGYRLIIVTG
                     RHHVAIHPFYQALALDTPAICCNGTYLYDYHAKTVLEADPMPVNKALQLIEMLNEHHI
                     HGLMYVDDAMVYEHPTGHVIRTSNWAQTLPPEQRPTFTQVASLAETAQQVNAVWKFAL
                     THDDLPQLQNFGKHVEHELGLECEWSWHDQVDIARGGNSKGKRLTKWVEAQGWSMENV
                     VAFGDNFNDISMLEAAGTGVAMGNADDAVKARANIVIGDNTTDSIAQFIYSHLI"
     gene            complement(2926..3984)
                     /gene="modC"
                     /locus_tag="J1Y83_003380"
     CDS             complement(2926..3984)
                     /gene="modC"
                     /locus_tag="J1Y83_003380"
                     /EC_number="7.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum ABC transporter ATP-binding protein
                     ModC"
                     /protein_id="HBA7631496.1"
                     /translation="MLELNFSQTLGNHCLTINETLPANGITAIFGVSGAGKTSLINAI
                     SGLTRPQKGRIVLNGRVLNDADKGICLTPEKRRVGYVFQDARLFPHYKVRGNLRYGMA
                     KSMVNQFDKLVALLGIEPLLDRLPGSLSGGEKQRVAIGRALLTAPELLLLDEPLASLD
                     IPRKRELLPYLQRLTREINIPMLYVSHSLDEILHLADRVMVLENGQVKAFGALEEVWG
                     SSVMNPWLPKEQQSSILKVTVLEHHPHYAMTALALGDQHLWVNKLDEPLQAALRIRIQ
                     ASDVSLVLQPPQQTSIRNVLRAKVVNSYDDNGQVEVELEVGGKTLWARISPWARDELA
                     IKPGLWLYAQIKSVSITA"
     gene            complement(3987..4676)
                     /gene="modB"
                     /locus_tag="J1Y83_003381"
     CDS             complement(3987..4676)
                     /gene="modB"
                     /locus_tag="J1Y83_003381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter permease subunit"
                     /protein_id="HBA7631497.1"
                     /translation="MILTDPEWQAVLLSLKVSSLAVLFSLPFGIFFAWLLVRCTFPGK
                     ALLDSVLHLPLVLPPVVVGYLLLVSMGRRGFIGERLYDWFGLSFAFSWRGAVLAAAVM
                     SFPLMVRAIRLALEGVDIKLEQAARTLGAGRWRVFFTITLPLTLPGIIVGTVLAFARS
                     LGEFGATITFVSNIPGETRTIPSAMYTLIQTPGGESGAARLCIISIALAMISLLISEW
                     LARISRERAGR"
     gene            complement(4676..5449)
                     /gene="modA"
                     /locus_tag="J1Y83_003382"
     CDS             complement(4676..5449)
                     /gene="modA"
                     /locus_tag="J1Y83_003382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HBA7631498.1"
                     /translation="MARKWLNLFTGAALSFAVAGNALADEGKITVFAAASLTNAMQDI
                     ATQYKKEKGVDVVSSFASSSTLARQIEAGAPADLFISADQKWMDYAVDKKAIDTASRQ
                     TLLGNSLVVVAPKASEQKDFTIDSKTNWTSLLNGGRLAVGDPEHVPAGIYAKEALQKL
                     GAWDTLSPKLAPAEDVRGALALVERNEAPLGIVYGSDAVASKGVKVVATFPEDSHKKV
                     EYPVAVVEGHNNATVKAFYDYLKGPQAAEIFKRYGFTTK"
     gene            complement(5615..5764)
                     /locus_tag="J1Y83_003383"
     CDS             complement(5615..5764)
                     /locus_tag="J1Y83_003383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux pump-associated protein, AcrZ
                     family"
                     /protein_id="HBA7631499.1"
                     /translation="MLELLKSLVFAVIMVPVVMAIILGLIYGLGEVFNIFSGVGKKDQ
                     PGQNH"
     gene            5893..6681
                     /gene="modE"
                     /locus_tag="J1Y83_003384"
     CDS             5893..6681
                     /gene="modE"
                     /locus_tag="J1Y83_003384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum-dependent transcriptional regulator"
                     /protein_id="HBA7631500.1"
                     /translation="MQAEILLTLKLQQKLFADPRRISLLKHIALSGSISQGAKDAGIS
                     YKSAWDAINEMNQLSEHILVERATGGKGGGGAVLTRYGQRLIQLYDLLAQIQQKAFDV
                     LSDDDALPLNSLLAAISRFSLQTSARNQWFGTITARDHDDVQQHVDVLLADGKTRLKV
                     AITTQSGARLGLDEGKEVLILLKAPWVGITQDEAVAQNADNQLPGIISHIERGAEQCE
                     VLMALPDGQTLCATVPVNEATSLQQGQNVTAYFNADSVIIATLC"
     gene            6749..8221
                     /gene="modF"
                     /locus_tag="J1Y83_003385"
     CDS             6749..8221
                     /gene="modF"
                     /locus_tag="J1Y83_003385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter ATP-binding protein
                     ModF"
                     /protein_id="HBA7631501.1"
                     /translation="MSSLQILQGTFRLSDTKTLQLPQLTLNAGDSWAFVGSNGSGKSA
                     LARAQAGELPLLKGERQSQFSHITRLSFEQLQKLVSDEWQRNNTDMLGPGEDDTGRTT
                     AEIIQDEVKDAPRCMQLAQQFGITALLDRRFKYLSTGETRKTLLCQALMSEPDLLILD
                     EPFDGLDVASRQQLAELLASLHQSGITLVLVLNRFDEIPEFVQFAGVLADCTLAETGA
                     KEELLQQALVAQLAHSELLVGVQMPEPDEPSARHALPANEPRIVLNNGVVSYNDRPIL
                     NNLSWQVNPGEHWQIVGPNGAGKSTLLSLITGDHPQGYSNDLTLFGRRRGSGETIWDI
                     KKHIGYVSSSLHLDYRVSTTVRNVILSGYFDSIGIYQAVSDRQQKLVQQWLDILGIDK
                     RTADAPFHSLSWGQQRLALIVRALVKHPTLLILDEPLQGLDPLNRQLIRRFVDVLISE
                     GETQLLFVSHHAEDAPACITHRLEFVPDGDFYRYALTKIN"
     gene            8483..9499
                     /gene="galE"
                     /locus_tag="J1Y83_003386"
     CDS             8483..9499
                     /gene="galE"
                     /locus_tag="J1Y83_003386"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706482.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HBA7631502.1"
                     /translation="MRVLVTGGSGYIGSHTCVQLLQNGHDVIILDNLCNSKRSVLPVI
                     ERLGGKHPTFVEGDIRNEALMTEILHDHAIDTVIHFAGLKAVGESVQKPLEYYDNNVN
                     GTLRLISAMRAANVKNFIFSSSATVYGDQPQIPYVESFPTGTPQSPYGKSKLMVEQIL
                     TDLQKAQPDWSIALLRYFNPVGAHPSGDMGEDPQGIPNNLMPYIAQVAVGRRDSLAIF
                     GNDYPTEDGTGVRDYIHVMDLADGHVVAMEKLANKPGVHIYNLGAGVGSSVLDVVNAF
                     SKACGKPVNYHFAPRREGDLPAYWADASKADRELNWRVTRTLDEMAQDTWHWQSRHPQ
                     GYPD"
     gene            9509..10555
                     /gene="galT"
                     /locus_tag="J1Y83_003387"
     CDS             9509..10555
                     /gene="galT"
                     /locus_tag="J1Y83_003387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-phosphate uridylyltransferase"
                     /protein_id="HBA7631503.1"
                     /translation="MTQFNPVDHPHRRYNPLTGQWILVSPHRAKRPWQGAQETPAKQV
                     LPAHDPDCFLCAGNVRVTGDKNPDYTGTYVFTNDFAALMSDTPDAPESNDPLMRCQSA
                     RGTSRVICFSPDHSKTLPELSVAALTEIVKTWQEQTAELGKTYPWVQVFENKGAAMGC
                     SNPHPHGQIWANSFLPNEAEREDRLQKEYFAEQKSPMLVDYVQRELADSSRTVVETEH
                     WLAVVPYWAAWPFETLLLPKAHVLRITDLTDAQRSDLALALKKLTSRYDNLFQCSFPY
                     SMGWHGAPFNGEENQHWQLHAHFYPPLLRSATVRKFMVGYEMLAETQRDLTAEQAAER
                     LRAVSDIHFRESGV"
     gene            10559..11707
                     /gene="galK"
                     /locus_tag="J1Y83_003388"
     CDS             10559..11707
                     /gene="galK"
                     /locus_tag="J1Y83_003388"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001713229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HBA7631504.1"
                     /translation="MSLKEKTQSLFANAFGYPATHTIQAPGRVNLIGEHTDYNDGFVL
                     PCAIDYQTVISCAPRDDRKVRVMAADYENQLDEFSLDAPIVAHENYQWANYVRGVVKH
                     LQLRNNSFGGVDMVISGNVPQGAGLSSSASLEVAVGTVLQQLYHLPLDGAQIALNGQE
                     AENQFVGCNCGIMDQLISALGKKDHALLIDCRSLGTKAVSMPKGVAVVIINSNFKRTL
                     VGSEYNTRREQCETGARFFQQPALRDVTIEEFNAVAHELDPIVAKRVRHILTENARTV
                     EAASALEQGDLKRMGELMAESHASMRDDFEITVPQIDTLVEIVKAVIGDKGGVRMTGG
                     GFGGCIVALIPEELVPAVQQAVAEQYEAKTGIKETFYVCKPSQGAGQC"
     gene            11701..12741
                     /gene="galM"
                     /locus_tag="J1Y83_003389"
     CDS             11701..12741
                     /gene="galM"
                     /locus_tag="J1Y83_003389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-epimerase"
                     /protein_id="HBA7631505.1"
                     /translation="MLNETPALAPDGQPYRLLTLRNNAGMVVTLMDWGATLLSARIPL
                     SDGSVREALLGCASPECYQDQAAFLGASIGRYANRIANSRYTFDGETVTLSPSQGVNQ
                     LHGGPEGFDKRRWQIVNQNDRQVLFALSSDDGDQGFPGNLGATVQYRLTDDNRISITY
                     RATVDKPCPVNMTNHVYFNLDGEQSDVRNHKLQILAEEYLPVDEGGIPHDGLKSVAGT
                     SFDFRNAKIIASEFLADDDQRKVKGYDHAFLLQAKGDGKKVAAHVWSADEKLQLKVYT
                     TAPALQFYSGNFLGGTPSRGTEPYADWQGLALESEFLPDSPNHPEWPQPDCFLRPGEE
                     YSSLTEYQFIAQ"
     gene            12944..13696
                     /gene="gpmA"
                     /locus_tag="J1Y83_003390"
     CDS             12944..13696
                     /gene="gpmA"
                     /locus_tag="J1Y83_003390"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000301556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HBA7631506.1"
                     /translation="MAVTKLVLVRHGESQWNKENRFTGWYDVDLSEKGVSEAKAAGKL
                     LKEEGYSFDFAYTSVLKRAIHTLWNVLDELDQAWLPVEKSWKLNERHYGALQGLNKAE
                     TAEKYGDEQVKQWRRGFAVTPPELTKDDERYPGHDPRYAKLSEKELPLTESLALTIDR
                     VIPYWNETILPRMKSGERVIIAAHGNSLRALVKYLDNMSEEEILELNIPTGVPLVYEF
                     DENFKPLKRYYLGNADEIAAKAAAVANQGKAK"
BASE COUNT         3480 a         3627 c         3499 g         3185 t
ORIGIN      
        1 actggcagag tccagttcat ttaacaatgc tgctaaatta ctcaatatta cgcaacctgc
       61 attaacgcga agaataaaaa agatggagga agatttacac attcaactat ttgagcgtac
      121 aactcgcaag gttacgttaa caaaagcagg aaaaaggttg ctcccggagg cgcgggaatt
      181 aataaaaaaa ttcgatgaaa cactttttaa tattcgtgat atgaatgctt atcatcgtgg
      241 tatggtgacg ttagcatgta ttccgaccgc agtattttat tttttaccat tggcaatcgg
      301 taaatttaat gagctgtatc ccaatattaa agtgcggatt ctggaacaag gcacgaataa
      361 ttgcatggaa tcggtgctgt gtaacgaatc agactttggc ataaacatga acaacgtcac
      421 aaattcatcc attgatttta ccccactggt caacgagccg ttcgtgctgg cctgtcggcg
      481 tgaccatcca ttggccaaaa agcagcttgt agaatggcag gagttagtgg gttacaaaat
      541 gattggcgta cgctcctcca gcggcaaccg actattgata gaacaacagc tagccgacaa
      601 gccctggaag ctggactggt tttacgaagt gcgtcatctt tcgacgtcat tagggctggt
      661 ggaggcggga ctggggattt cggcgcttcc tggtctggca atgccccatg cgccctattc
      721 ttcgattatt ggtatcccac tggtggaacc cgttatacgt cggacattgg ggattattcg
      781 ccgcaaagat gccgtgcttt ctccggcagc tgaacgcttt tttgccttgc tgattaattt
      841 gtggactgac gataaagaca atttgtggac caatatcgtc caacgccaga gacatgcgtt
      901 acaggagata ggttaataca ccggcgcagg agagactgcg ccggttaaat cagcggttag
      961 tgcgcgttga ccactaccca cattggcccc tgcccaaccg catagcggcc tttttcatgc
     1021 agtagcccct gctcgccaac aatttcgtat accgagatgt ggtgagattt ttgcccggcg
     1081 gcaatcagat acttgccgct gtgatcaaca ttgaagccgc gcggctgggt ttccgttggc
     1141 tggaagcctt ctttactcaa cacgctgcca tcttccgaaa cgctgaaaac ggtaatcaga
     1201 ctggcggtac ggtcgcaggc gtataaatga cgaccatccg gggtgatatg aatatcagcc
     1261 gcccaacggg tgtcggagaa gttttccggc atcatatcca gcgtctggac acattcgata
     1321 ttgccgtgcg gatctttcag ttcccagaca tccactgagc tgtttaactc attgacgcaa
     1381 tacgcatatt gttcgtttgg atggaatacc atatgacgcg ggccggcccc ttcaacggta
     1441 gtcacttccg cagggtcctg cgctaccaga tgaccatcat cgctgaccgt aaacaggcaa
     1501 atgcgatcct gctttaatgc cggaacccac aacgtacggt tgtccggtga gatattggcg
     1561 gaatggcaac cgtccagccc ctcgaccaca tcgacgacgc ccactggcag gccatcttcc
     1621 agacgcgtta cgctcacgtt acccgcatta taagaaccga caaagacaaa ctgccccagg
     1681 tgatcggtgg aaatatgcgt cgggctaccc ggcagcgcag actctgcagc aaaggtcagc
     1741 gcgccatcgt ccggtgcaat acgatacgcc agaacacgaa actccggacg gacaccaacg
     1801 tagaggtaac gtttgtccgg gctgaccacc atcggctgca cctgccccgg cacatcgaca
     1861 acctgtgtca gcgtcagtgc gccttcatga ttcaggttcc agacgtgaat ttgctggctc
     1921 tcagggctgg cgatataaac tgtttgcttc atgaatgctc ctttgcattt agcaactgac
     1981 tgtaacagct aaaattagtc gcttttggcg gtgaatgctg aattaaaacg cagaattttg
     2041 tccgatagtg gtatcggtgt accattcgcg cagacgaaaa ttcttaacct taatctggaa
     2101 tacgccatga ccacacgcgt gattgctctc gacttagacg gcaccttatt gaccccgaaa
     2161 aagaccctgc ttccttcatc gatagaagcc ctggcccgtg ctcgcgaagc aggctaccga
     2221 ttaatcatcg tcacaggtcg ccatcacgtc gctattcatc ctttttatca ggcgctggcg
     2281 ctggatacac ctgctatttg ctgtaacggc acctatttgt atgattatca tgcaaaaacc
     2341 gtactagaag cggacccaat gcctgttaac aaagccctgc aactcattga gatgctgaat
     2401 gaacaccaca ttcacggtct gatgtatgtg gatgatgcga tggtttatga gcacccgacc
     2461 ggacatgtca ttcgcacgtc taactgggcg caaaccctgc cgccggaaca gcgtccgact
     2521 ttcacacaag tcgcttctct ggctgaaacg gcgcaacaag ttaacgccgt atggaagttc
     2581 gccctgacgc acgatgacct gccgcaattg cagaattttg gtaagcatgt cgaacatgaa
     2641 ctgggactgg agtgtgaatg gtcctggcac gatcaggttg atattgcacg cggcggcaac
     2701 agcaaaggta aacgtttgac gaaatgggtt gaggcgcaag gctggtcgat ggaaaacgtc
     2761 gtggccttcg gcgataactt caatgatatt agtatgctgg aagccgctgg tacaggcgtg
     2821 gcgatgggca acgccgatga cgcagtaaaa gcgcgcgcca acattgtgat tggtgataac
     2881 accaccgaca gcattgccca gttcatttat agccacctga tttaatcagg cggttatcga
     2941 cacactttta atttgcgcat acagccacag gccaggtttg atcgccagtt catccctggc
     3001 ccacgggctg atacgcgccc acagcgtttt accgccgact tccagctcca cttccacctg
     3061 gccgttgtcg tcataactat taacaacttt tgcccgcaat acgttacgaa tgctggtttg
     3121 ctgtggcggt tgtaaaacca gcgagacatc ggaagcctga atgcggatgc gtagcgcagc
     3181 ttgcagcggt tcgtccagct tattgaccca caaatgctga tcgcccagcg ccagcgcggt
     3241 catcgcgtaa tgcggatgat gctcaagtac tgtcactttc agaatgctac tttgttgctc
     3301 tttcggtagc cacggattca tcacgctact accccagact tcttccagcg cgccaaaggc
     3361 tttcacctga ccgttttcca gtaccatcac tctgtctgcc agatggagga tctcatccag
     3421 cgaatggctg acatacaaca tcggaatgtt gatttcccgc gtcagacgtt gcagataagg
     3481 caacagttcg cgtttacgcg gaatatccag tgacgccagc ggttcatcca gcaacagcaa
     3541 ttccggtgct gtcagcaaag cccgaccaat cgccacgcgc tgtttttcgc ctccggacag
     3601 gctgcctggt aaacgatcaa gcaacggttc aatgcctaaa agcgccacca gcttatcgaa
     3661 ctgattgacc atgcttttcg ccatgccgta gcgcaaattg ccacgcactt tataatgcgg
     3721 aaacagccgc gcatcctgaa acacatagcc tacgcgacgc ttttctggcg tcagacagat
     3781 acctttatcg gcgtcattta gtacccgacc attgaggaca atccgccctt tttgcggacg
     3841 tgtcagccca ctgatggcgt taatcagcga ggtttttccg gcaccagaga caccaaaaat
     3901 agcggtgatg ccattggcgg gcagcgtttc attaatcgtc aggcaatgat tgcccaacgt
     3961 ctgggaaaaa ttcagttcca gcatgattag cgccccgccc gttcacggct gattctggcc
     4021 agccattctg aaatcaacag ggagatcatc gccagcgcaa tagaaataat acacagtctc
     4081 gccgcgccac tttccccgcc aggggtctgg atcagggtat acatggcaga aggaatggtt
     4141 cgcgtttcac caggaatgtt cgacacaaag gtgatggttg cgccaaattc accgagcgaa
     4201 cgagcgaatg ccagcaccgt accaacaata attcccggta aggtcagcgg taacgtgata
     4261 gtaaagaaaa cgcgccagcg cccggccccc agtgttcttg cggcctgctc cagtttgata
     4321 tcaacacctt ccagcgccag acgaatcgcc cgcaccatca gcggaaaaga catgacagcg
     4381 gcagcgagaa ccgcaccgcg ccagctaaac gcgaaagaaa gaccaaacca gtcatacaga
     4441 cgttcaccga taaatccgcg ccgtcccatc gaaactaata ataggtaacc gacgaccacg
     4501 ggcggtaaca ccagcggtag atgcagtacg ctgtcgagca gagctttgcc cggaaacgtg
     4561 caacgtacca gtaaccaggc aaaaaagatc ccaaacggca ggctaaacag cacagccagg
     4621 gaagaaactt tcaggcttaa taaaactgcc tgccattctg gatcggtcag tatcattact
     4681 tggttgtaaa tccgtaacgt ttaaagattt ccgcagcctg cggccccttc agataatcgt
     4741 aaaatgcttt cactgtcgca ttgttatgcc cttccacaac agcaaccgga tattccactt
     4801 ttttatgtga atcttccggg aaggtggcaa ccacttttac ccctttgctg gcaactgcgt
     4861 cagaaccgta aacaataccc agcggcgctt cattgcgttc gaccaacgcc agcgcgccac
     4921 gaacatcttc cgccggagcc agtttcgggg agagcgtatc ccatgcgccc agtttttgca
     4981 gtgcttcttt cgcataaatg ccagcaggga catgttccgg atcaccaacc gcgaggcgac
     5041 cgccattcag cagcgaagtc cagttggttt tgctgtcgat ggtgaaatct ttctgctcgc
     5101 tggctttcgg tgctacaacg accaggctat tgccgagcag tgtctggcgc gaagccgtat
     5161 cgatcgcttt tttatcaacc gcataatcca tccatttctg atcggcagaa ataaatagat
     5221 ccgcaggcgc acccgcttca atctgacggg ccaaagtaga tgacgaagcg aaagaagaaa
     5281 ccacatccac gcctttctct tttttatact gcgtagcaat gtcctgcatt gcgttagtca
     5341 gtgatgcggc ggcgaacacc gtgattttcc cttcatctgc cagtgcattg ccagcaacag
     5401 cgaaagagag tgccgccccg gtaaacaagt tcaaccattt acgagccatt ggtaacccct
     5461 taatgtaacg ttatgtaggc gtcaatataa cggcaagttc ggaattttcc cagcagttat
     5521 tgctaacctt tgtgaggtag atgagaaaaa atatcggcag gaaaagcagg aggttgagaa
     5581 aaagaaatgc ccgactaagc gggcattcag ggattcaatg attttgtccg ggctggtctt
     5641 ttttaccaac accagaaaag atgttgaata cttcaccaag accgtaaatc agacccagga
     5701 tgatggccat cacgacaggt accatgatta cggcgaatac cagacttttt aataactcta
     5761 acatggtcaa ctccagatat agtcatgaga ctattctaac cgctaagcaa agaaaagcac
     5821 tccccttttg tgcggtcagc tttgcgtggc gttcgttttc cgtcacaata agactttttc
     5881 caggatattg ttatgcaggc cgaaatcctt ctcaccctta agctccaaca aaaattattc
     5941 gccgacccgc gccgcatttc gctactaaaa cacattgcgc tttccggttc cattagccag
     6001 ggagcgaaag atgccggtat cagctataaa agcgcctggg atgccattaa cgagatgaat
     6061 cagttaagtg agcatattct ggttgagcgc gcaacaggcg gtaaaggtgg aggcggcgca
     6121 gtactgaccc gctatggtca gcgactgatt cagctctatg acttactggc gcaaatccag
     6181 caaaaagcct ttgatgtgtt aagtgacgat gacgccctgc cgctgaacag cctgctggcc
     6241 gcgatctcac gtttttcact gcaaaccagc gcccgtaacc aatggtttgg caccatcacc
     6301 gcccgcgatc atgatgacgt tcaacagcat gttgatgtct tactggctga cggaaaaaca
     6361 cgcctgaaag tcgcaattac cacacaaagc ggcgcgcgtc tggggctgga tgaaggcaaa
     6421 gaagtgttga tattgctaaa agcgccgtgg gtaggcatta ctcaggacga agcggtcgca
     6481 caaaacgccg acaaccaatt accgggtatt attagccata ttgagcgcgg cgcagagcag
     6541 tgcgaagtat taatggcgct acccgacggg caaacactgt gcgccacagt gccggtaaat
     6601 gaagcgactt ctcttcagca aggacagaat gtcacggcct actttaatgc cgacagcgtg
     6661 attatcgcca cgctgtgcta agcgtgttga caatttgtta tgaaacacgt atccctgtca
     6721 gtaatcgctg cacaaaatgg gatataaaat gtcatcgttg caaattttgc aaggcacgtt
     6781 tcgtcttagc gacacaaaaa cgctgcaatt gcctcagcta acgttaaacg cgggtgatag
     6841 ttgggcgttt gtcggttcga atggaagcgg gaaatcggcg ctggcccgcg cgcaggcggg
     6901 ggaacttccg cttttgaaag gtgaacggca aagccagttt tcccacatca ctcgtctctc
     6961 cttcgagcaa ttgcaaaagc tcgtcagcga cgaatggcag cggaataaca ccgatatgct
     7021 cggccctggc gaagatgaca ccgggcgtac cacggctgaa atcattcagg atgaagtaaa
     7081 ggatgcaccg cgttgcatgc aactggcgca gcagttcggt attaccgccc tcctcgaccg
     7141 acgctttaaa tacctttcca ctggcgagac gcgaaaaacc ctgctgtgtc aggcgctgat
     7201 gtcggagcct gacttgttga ttcttgatga gccgttcgat ggcctggatg ttgcctcacg
     7261 tcagcagctg gctgagctac tcgcctcgtt acatcagtcc ggtattactc tggtactggt
     7321 gctcaatcgc ttcgatgaga tcccggaatt tgtccagttt gctggcgtgc tggcggattg
     7381 cacgttagcg gaaactggcg ctaaagaaga actgctccaa caagcactcg ttgcgcaact
     7441 ggcgcatagc gaactgcttg taggtgtgca aatgccggag ccagatgaac cttcagcacg
     7501 tcacgcctta cccgccaacg aaccgcgcat tgtgctgaac aatggcgtgg tttcttataa
     7561 cgatcgcccc attcttaata accttagctg gcaggtgaat ccaggcgaac actggcaaat
     7621 tgtcgggcca aatggcgcgg gaaaatcgac gttattaagc ctgattactg gcgatcatcc
     7681 gcaaggttac agcaacgatt tgacgctttt cggacgacgt cgcggcagcg gcgaaaccat
     7741 ctgggatatc aaaaagcata tcggttacgt cagcagtagt ttgcatctgg attaccgggt
     7801 cagcactact gtgcgtaatg tgattctttc tggctatttt gattcgattg gcatttatca
     7861 ggccgtttcg gatcgccagc aaaaactggt gcagcagtgg ctggatattc tcggcattga
     7921 taaacgcacg gctgacgctc ctttccatag tctttcctgg ggacagcagc gtctggcgct
     7981 gatcgtccgt gcgctagtga aacatccgac gttgcttatt ctcgatgaac cactacaggg
     8041 gcttgatccg ctcaatcgcc agcttatccg ccgttttgtt gatgtgctga ttagcgaagg
     8101 tgaaacgcaa ttgttgtttg tttcgcacca cgctgaagat gcgcctgcct gtattaccca
     8161 tcgtcttgag ttcgtgccgg acggtgactt ttatcgctat gcgctgacaa aaataaactg
     8221 agtcggtagt gctgaccgtg ccggaggcgg cgtaaacgtg ttatccggcc aacggtttga
     8281 cgcatgcagg catgaaaccg cgtctttttt tcagataaaa agcgcaatca ttcataaacc
     8341 ctctgtttta taatcactta atcgcgcata aaaaacggct aaattcttgt gtaaacgatt
     8401 ccactaattt attccatgtc acacttttcg catctttgtt atgctatggt tatttcatac
     8461 cataagccta atggagcgaa ttatgagagt tctggttacc ggtggtagcg gttacattgg
     8521 aagtcatacc tgtgtgcaat tactgcaaaa cggtcatgat gtcatcattc ttgataacct
     8581 ctgtaacagt aagcgcagcg tactgcctgt tatcgagcgt ttaggcggca aacatccaac
     8641 gtttgttgaa ggcgatatcc gtaacgaagc gttgatgacc gagatcctgc acgatcacgc
     8701 tatcgacacc gtgatccact tcgccgggct gaaagccgtt ggcgaatcgg tacaaaaacc
     8761 gcttgaatat tacgacaaca atgtcaacgg tactctgcgc ctgattagcg ccatgcgcgc
     8821 cgctaacgtc aaaaacttta tctttagctc ctccgccacc gtttatggcg atcaacccca
     8881 aattccatat gttgaaagct tcccgaccgg cacaccgcaa agcccttacg gcaaaagcaa
     8941 gctgatggtg gaacagatcc tcaccgatct gcaaaaagcc cagccggact ggagcattgc
     9001 cctgctgcgc tacttcaacc cagttggcgc gcatccgtcg ggcgatatgg gcgaagatcc
     9061 gcaaggcatt ccgaataacc tgatgccata catcgcccag gttgctgtag gccgtcgcga
     9121 ctcgctggcg atttttggta acgattatcc gaccgaagac ggtactggcg tgcgcgatta
     9181 catccacgta atggatctgg cggacggtca cgtcgtggcg atggaaaaac tggcgaacaa
     9241 gccaggcgta cacatctaca acctcggcgc tggcgtgggc agcagcgtgc tggatgtggt
     9301 taatgccttc agcaaagctt gcggcaaacc ggttaactat cattttgcac cgcgtcgcga
     9361 gggcgacctt ccggcctact gggcggacgc cagcaaagcc gaccgtgaac tgaactggcg
     9421 cgtaacgcgc acactcgatg aaatggcgca ggacacctgg cactggcagt cacgccatcc
     9481 acagggatat cccgattaag gaacgaccat gacgcaattt aatcccgttg atcatccaca
     9541 tcgccgctac aacccgctca ccgggcaatg gattctggtt tcaccgcacc gcgctaagcg
     9601 cccctggcag ggggcgcagg aaacgccagc caaacaggtg ttacctgcgc acgatccaga
     9661 ttgcttcctc tgcgcaggta atgtgcgggt gacaggcgat aaaaaccccg attacaccgg
     9721 cacttacgtt ttcactaatg actttgcggc cttgatgtct gacacgccag atgcgccaga
     9781 aagcaacgat ccgctaatgc gttgccagag cgcgcgcggt accagccggg tgatctgctt
     9841 ttcaccggat cacagtaaaa cgttgccaga actgagcgtt gcggcattga cggaaatcgt
     9901 caaaacctgg caggagcaaa ccgcagagct gggaaaaacg tacccgtggg tacaggtctt
     9961 tgaaaacaaa ggcgcggcga tgggctgctc taacccgcat ccgcacggac agatttgggc
    10021 aaatagcttc ctgcctaacg aagctgagcg cgaagaccgc ctgcaaaaag aatattttgc
    10081 cgaacagaaa tcaccaatgc tggtggatta tgttcagcgc gagctggcag acagtagccg
    10141 taccgttgtc gaaaccgaac actggttagc cgttgtacct tactgggctg cctggccgtt
    10201 cgaaacgcta ctgctgccca aagcccacgt tttgcggatc accgatttga ccgacgccca
    10261 gcgcagcgat ttggcactgg cgttgaaaaa gctaaccagt cgttatgaca acctcttcca
    10321 gtgctccttc ccctactcta tgggctggca cggcgcgcca tttaatggcg aagagaatca
    10381 acactggcag ctgcacgcgc acttttatcc gcctctgttg cgctccgcca ccgtacgtaa
    10441 atttatggtt ggttatgaaa tgctggcaga aacccagcga gacctgaccg cagaacaggc
    10501 agcagagcgt ttgcgcgcag tcagcgatat ccattttcgc gaatccggag tgtaagaaat
    10561 gagtctgaaa gaaaaaacac aatctctgtt tgccaacgca tttggctacc ctgccactca
    10621 caccattcag gcgcctggcc gcgtgaattt gattggtgaa cacaccgact acaacgacgg
    10681 tttcgttctg ccctgcgcga ttgattatca aaccgtgatc agctgtgcgc cacgcgatga
    10741 ccgtaaagtt cgcgtaatgg cagccgatta tgaaaatcag ctcgacgagt tttccctcga
    10801 tgcgcccatt gtcgcgcatg aaaactatca atgggcgaac tacgttcgtg gcgtggtgaa
    10861 acatctgcaa ctgcgtaaca acagcttcgg cggtgtggac atggtgatca gcggcaatgt
    10921 gccgcagggt gcagggttaa gttcttccgc ttcactggaa gtcgcggtcg gaaccgtatt
    10981 gcagcagctt tatcatctgc cgctggacgg cgcacaaatc gcgcttaacg gtcaggaagc
    11041 agaaaaccag tttgttggct gtaactgcgg gatcatggat cagctaattt ccgcactcgg
    11101 caagaaagat catgccttgc tgattgactg ccgctcactg gggaccaaag cagtttccat
    11161 gcccaaaggt gtggctgtcg tcattatcaa cagtaacttc aaacgtaccc tggttggcag
    11221 cgaatacaac acccgtcgtg aacagtgcga aaccggtgcg cgtttcttcc agcagccagc
    11281 cctgcgcgat gtcaccattg aagagttcaa cgctgttgcg catgaactgg acccaatcgt
    11341 ggcgaaacgc gtgcgtcata tcctgactga aaacgcccgc accgttgaag ctgccagcgc
    11401 actggagcaa ggcgacctga aacgtatggg cgagttgatg gcggagtctc atgcctctat
    11461 gcgcgatgat ttcgaaatca ccgtaccgca aattgacact ctggtagaaa tcgtcaaagc
    11521 tgtgattggc gacaaaggtg gcgtacgcat gaccggcggc ggatttggtg gctgtatcgt
    11581 cgcgttgatc ccggaagaac tggtgcctgc cgtacagcaa gctgtcgctg aacaatatga
    11641 agcaaaaaca ggtattaaag agacttttta cgtttgtaaa ccatcacaag gagcaggaca
    11701 gtgctgaacg aaactcccgc actggcaccc gatggtcagc cgtaccgact gttaactttg
    11761 cgtaacaacg cagggatggt agtcacgctg atggactggg gtgcgacttt actttccgcc
    11821 cgtattccgc tttccgatgg cagcgtccgc gaggcgctgc ttggctgcgc cagcccggaa
    11881 tgctatcagg atcaggccgc atttctgggg gcctctattg gtcgttatgc caaccgcatc
    11941 gccaatagcc gttatacctt tgacggtgaa accgtgacgc tttcaccaag tcagggcgtc
    12001 aaccaattgc atggcggccc ggaagggttc gacaaacgtc gctggcagat tgtgaaccag
    12061 aacgatcgtc aggtgctgtt tgccctgagt tcagatgatg gcgatcaggg tttcccgggt
    12121 aatctcggcg cgacggtgca atatcgtctg accgacgata accgtatctc cattacttat
    12181 cgcgccacag ttgataaacc ttgcccggtg aatatgacta atcacgtcta tttcaatctt
    12241 gacggcgagc agtctgacgt gcgcaatcac aagttgcaga ttctggcgga agaatatctg
    12301 ccggttgatg aaggcggcat tccgcacgac ggcctgaaat ctgtcgccgg aacctctttt
    12361 gatttccgca acgccaaaat catcgccagt gagtttcttg ccgacgacga tcagcgcaaa
    12421 gtgaaaggtt acgatcacgc gttcttgtta caggccaaag gcgatggcaa gaaagtggcg
    12481 gcgcatgtct ggtcagcaga tgaaaaattg cagctgaagg tctacaccac cgctccggct
    12541 ctgcaattct actccggcaa cttcctcggc ggcacaccgt cgcggggaac cgaaccttac
    12601 gccgactggc aagggctggc tctggaaagc gaatttctgc cggacagccc gaaccaccct
    12661 gaatggccgc aaccagactg cttcctgcgt cctggcgaag agtattccag cctgacggaa
    12721 tatcagttta ttgctcagta atgttaagcc ctcgtcatga gggctttatc ttcatattgt
    12781 tcaaatcacc agcaaacacc gacatatttg caactcaata ttcacaacaa ccttacactg
    12841 cgacactatt ttcgctatgg ttatgcgtaa gcattgctgt tgcttcgtca cggcaatata
    12901 atgagaatta ttatcattaa aagatgattt gaggagtaag tatatggctg taactaagct
    12961 ggttctggtt cgtcatggcg aaagtcagtg gaacaaagaa aaccgtttca ccggttggta
    13021 cgacgtggat ctgtctgaga aaggcgtaag cgaagcaaaa gcagcaggta agctgctgaa
    13081 agaggaaggc tacagctttg actttgctta cacctctgtg ctgaaacgcg ctattcatac
    13141 cctgtggaat gtgctggacg aactggatca ggcatggctg cccgttgaga aatcctggaa
    13201 actgaacgaa cgtcactacg gtgcgttgca gggtctgaac aaagcggaaa ccgctgaaaa
    13261 gtatggcgac gagcaggtga aacagtggcg tcgtggtttt gcggtgactc cgccggaact
    13321 gactaaagat gatgagcgtt atccgggtca cgatccgcgt tacgcgaaac tgagcgagaa
    13381 agaactgccg ctgacggaaa gcctggcgct gaccattgac cgcgtgatcc cttactggaa
    13441 tgaaaccatt ctgccgcgta tgaagagcgg tgagcgcgtc atcatcgccg cacacggtaa
    13501 ctcgttacgt gcgctggtga aatatctcga taacatgagc gaagaagaga ttcttgagct
    13561 gaatatcccg accggcgtgc cgctggttta tgagtttgac gagaacttca aaccgctgaa
    13621 acgctattat ctgggtaatg ctgacgagat cgcggcgaaa gcagcggcgg ttgctaacca
    13681 gggtaaagcg aagtaaacgt cattcgttta aaaatgagaa agccgaccgc aaaatgagtc
    13741 ggctttttta tttttcgttg ctaccaatgg agcacatcgc ctgatgcgac g
//
LOCUS       DADSYM010000129        13761 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2415
            SAMN08039579-rid11723173.denovo.130, whole genome shotgun sequence.
ACCESSION   DADSYM010000129 DADSYM010000000
VERSION     DADSYM010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039579
            Sequence Read Archive: SRR6362533
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13761)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13761)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980162.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 03:39:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,484
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 4,347
            CDSs (with protein)               :: 4,347
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 43 of 85
            Pseudo Genes (incomplete)         :: 37 of 85
            Pseudo Genes (internal stop)      :: 19 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13761
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039579-rid11723173.denovo.130"
                     /strain="LGE2415"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8302 N 95.9628 W"
                     /collection_date="2015-08-24"
     gene            complement(<1..1364)
                     /gene="emrY"
                     /locus_tag="J1Y83_003391"
     CDS             complement(<1..1364)
                     /gene="emrY"
                     /locus_tag="J1Y83_003391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter permease
                     subunit EmrY"
                     /protein_id="HBA7631507.1"
                     /translation="MAITKSTPAPLTGGTLWCVTIALSLATFMQMLDSTISNVAIPTI
                     SGFLGASTDEGTWVITSFGVANAIAIPVTGRLAQRIGELRLFLLSVTFFSLSSLMCSL
                     STNLDVLIFFRVVQGLMAGPLIPLSQSLLLRNYPPEKRTFALALWSMTVIIAPICGPI
                     LGGYICDNFSWGWIFLINVPMGIIVLTLCLTLLKGRETETSPVKMNLPGLTLLVLGVG
                     CLQIMLDKGRDLDWFNSSTIIILTVVSVISLISLVIWESTSENPILDLSLFKSRNFTI
                     GIVSITCAYLFYSGAIVLMPQLLQETMGYNAIWAGLAYAPIGIMPLLISPLIGRYGNK
                     IDMRLLVTFSFLMYAVCYYWRSVTFMPTIDFTGIILPQFFQGFAVACFFLPLTTISFS
                     GLPDNKFANASSMSNFFRTLSGSVGTSLTMTLWGRRESLHHSQLTATIDQFNPVFNSS
                     SQIM"
     gene            complement(1364..2527)
                     /gene="emrK"
                     /locus_tag="J1Y83_003392"
     CDS             complement(1364..2527)
                     /gene="emrK"
                     /locus_tag="J1Y83_003392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter periplasmic
                     adaptor subunit EmrK"
                     /protein_id="HBA7631508.1"
                     /translation="MEQINSNKKHSDRRKYFALLAVVLFIAFSGAYAYWSMELKDMIS
                     TDDAYVTGNADPISAQVSGSVTVVNHKDTNYVRQGDILVSLDKTDATIALNKAKNNLA
                     NIVRQTNKLYLQDKQYSAEVASARIQYQQSLEDYNRRVPLAKQGVISKEALEHTKDTL
                     ISSKAALNAAIQAYKANKALVMNTPLNRQPQVIEAADATKEAWLALKRTDIKSPVTGY
                     IAQRSVQVGETVSPGQSLMAVVPARQMWVNANFKETQLTDVRIGQSVNIISDLYGENV
                     VFHGRVTGINMGTGNAFSLLPAQNATGNWIKIVQRVPVEVSLDPKELMEHPLRIGLSM
                     TATIDTKNEDIAEMPDLASTVTSMPAYTSKALVIDTSPIEKEISNIISHNGQL"
     gene            2943..3557
                     /gene="evgA"
                     /locus_tag="J1Y83_003393"
     CDS             2943..3557
                     /gene="evgA"
                     /locus_tag="J1Y83_003393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-sensing system DNA-binding response
                     regulator EvgA"
                     /protein_id="HBA7631509.1"
                     /translation="MNAIIIDDHPLAIAAIRNLLIKNDIEILAELTEGGSAVQRVETL
                     KPDIVIIDVDIPGVNGIQVLETLRKRQYSGIIIIVSAKNDHFYGKHCADAGANGFVSK
                     KEGMNNIIAAIEAAKNGYCYFPFSLNRFVGSLTSDQQKLDSLSKQEISVMRYILDGKD
                     NNDIAEKMFISNKTVSTYKSRLMEKLECKSLMDLYTFAQRNKIG"
     gene            3562..7155
                     /gene="evgS"
                     /locus_tag="J1Y83_003394"
     CDS             3562..7155
                     /gene="evgS"
                     /locus_tag="J1Y83_003394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-sensing system histidine kinase EvgS"
                     /protein_id="HBA7631510.1"
                     /translation="MKFLPYIFLLCCGLWSTISFADGDYIEYRGISSNNRVTLDPLRL
                     SNKELRWLASKKNLVIAVHKSQTATLLHTDSQQRIRGINADYLNLLKRALNIKLTLRE
                     YADHQKAMDALEDGEVDIVLSHLVASPPLNDDIAATNPLIITFPALVTTLHDSMRPLT
                     SSKPVNIARVANYPPDEVIHQSFPKATIISFTNLYQALASVSAGQNDYFIGSNIITSS
                     MISRYFTHSLNVVKYYNSPRQYNFLLTRKDSIVLNEVLNRFVDALTNEVRYEVSQNWL
                     DTGNLAFLNKPLELTEHEKQWIKQHPDLKVLENPYSPPYSMTDETGSVRGVMGDILNI
                     ITLQTGLNFSPITVSHNIHAGTQLNPGGWDILPAAIYSEDRENNVSFAEVFITTPYVF
                     VMQKAPDSEQTLKKGMKVAIPYYYELHSQLKEMYPEVEWIKVDNASAAFHKVKEGELD
                     ALVATQLNSRYMIDHYYPNELYHFLIPGVQNASLSFAFPRGEPELKDIINKALNAIPP
                     SEVLRLTEKWIKMPNVTIDTWDLYSEQFYIVTTLSVLLVGSSLLWGFYLLRSVRRRKV
                     IQGDLENQISFRKALSDSLPNPTYVVNWQGNVISHNSAFEHYFTADYYKNAMLPLENS
                     ESPFKDVFSNTHEVTAETKENRTIYTQVFEIDNGIEKRCINHWHTLCNLPASEHAVYI
                     CGWQDITETRDLIHALEVERNKAINATVAKSQFLATMSHEIRTPISSIMGFLELLSGS
                     GLSKEQRVEAISLAYATGQSLLGLIGEILDVDKIESGNYQLQPQWVDIPTLVQNTCHS
                     FGAIAASKSIALSCSSTLPERYLVKIDPQAFKQVLSNLLSNALKFTTEGAVKITTSLG
                     HIDDNHAVIKMTIMDSGSGLSQEEQQQLFKRYSQTSAGRQQTGSGLGLMICKELIKNM
                     QGDLSLESHPGIGTIFTITIPVEITQQVAAVEAKAEQPITLPEKLSILIADDHPTNRL
                     LLKRQLNLLGYDVDEATDGVQALHKVSMQHYDLLITDVNMPNMDGFELTRKLREQNSS
                     LPIWGLTANAQANEREKGLNCGMNLCLFKPLTLDVLKTHLSQLHQVAHIAPQYRHLDI
                     EALKNNTANDLQLMQEILMTFQHETHKDLPAAFHALEAGDNRTFHQCIHRIHGAANIL
                     NLQKLINISHQLEITPVSDDSKPEILQLLNSVKEHIAELDQEITVFCQQND"
     gene            complement(7211..8356)
                     /gene="yfdE"
                     /locus_tag="J1Y83_003395"
     CDS             complement(7211..8356)
                     /gene="yfdE"
                     /locus_tag="J1Y83_003395"
                     /EC_number="2.8.3.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416872.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA:oxalate CoA-transferase"
                     /protein_id="HBA7631511.1"
                     /translation="MTNNECKGPFEGLLVIDMTHVLNGPFGTQLLCNMGARVIKVEPP
                     GHGDDTRTFGPYVDGQSLYYSFINHGKESVVLDLKNDHDKSIFINMLKQADVLAENFR
                     PGTMEKLGFSWERLQEINPRLIYASSSGFGHTGPLKDAPAYDTIIQAMSGIMMETGYP
                     DAPPVRVGTSLADLCGGVYLFSGIVSALYGREKSQRGAHVDIAMFDATLSFLEHGLMA
                     YIATGKSPQRLGNRHPYMAPFDVFDTQDKPITICCGNDKLFSALCQALELTELVNDPR
                     FSSNILRVHNQAILKEYIERTLKTQVAEVWLARIHEVGVPVAPLLSVAEAINLPQTQA
                     RNMLIEAGGIMMPGNPIKISGCADPHVMPGAATLDQHGEQIRQEFSS"
     gene            complement(8430..9374)
                     /gene="yfdV"
                     /locus_tag="J1Y83_003396"
     CDS             complement(8430..9374)
                     /gene="yfdV"
                     /locus_tag="J1Y83_003396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter YfdV"
                     /protein_id="HBA7631512.1"
                     /translation="MLTFFIGDLLPIIVIMLLGYFSGRRETFSEDQARAFNKLVLNYA
                     LPAALFVSITRANREMIFADTRLTLVSLVVIVGCFFFSWFGCYKFFKRTHAEAAVCAL
                     IAGSPTIGFLGFAVLDPIYGDSVSTGLVVAIISIIVNAITIPIGLYLLNPSSGADGKK
                     NSNLSALISAAKEPVVWAPVLATILVLVGVRIPAAWDPTFNLIAKANSGVAVFAAGLT
                     LAAHKFEFSAEIAYNTFLKLILMPLALLLVGMACHLNSEHLQMMVLAGALPPAFSGII
                     IASRFNVYTRTGTASLAVSVLGFVVTAPLWIYVSRLVS"
     gene            complement(9444..11138)
                     /gene="oxc"
                     /locus_tag="J1Y83_003397"
     CDS             complement(9444..11138)
                     /gene="oxc"
                     /locus_tag="J1Y83_003397"
                     /EC_number="4.1.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxalyl-CoA decarboxylase"
                     /protein_id="HBA7631513.1"
                     /translation="MSDQLQMTDGMHIIVEALKQNNIDTIYGVVGIPVTDMARHAQAE
                     GIRYIGFRHEQSAGYAAAASGFLTQKPGICLTVSAPGFLNGLTALANATVNGFPMIMI
                     SGSSDRAIVDLQQGDYEELDQMNAAKPYAKAAFRVNQPQDLGIALARAIRVSVSGRPG
                     GVYLDLPANVLAATMEKDEALTTIVKVENPSPALLPCPKSVTSAISLLAKAERPLIIL
                     GKGAAYSQSDEQLREFIESAQIPFLPMSMAKGILEDTHPLSAAAARSFALANADVVML
                     VGARLNWLLAHGKKGWAADTQFIQLDIEPQEIDSNRPIAVPVVGDIASSMQGMLAELK
                     QNTFTTPLVWRDILNIHKQQNAQKMHEKLSTDTQPLNYFNALSAVRDVLRENQDIYLV
                     NEGANTLDNARNIIDMYKPRRRLDCGTWGVMGIGMGYAIGASVTSGSPVVAIEGDSAF
                     GFSGMEIETICRYNLPVTIVIFNNGGIYRGDGVDLSGAGAPSPTDLLHHARYDKLMDA
                     FRGVGYNVTTTDELRHALTTGIQSRKPTIINVVIDPAAGTESGHITKLNPKQVAGN"
     gene            complement(11192..12442)
                     /gene="frc"
                     /locus_tag="J1Y83_003398"
     CDS             complement(11192..12442)
                     /gene="frc"
                     /locus_tag="J1Y83_003398"
                     /EC_number="2.8.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formyl-CoA transferase"
                     /protein_id="HBA7631514.1"
                     /translation="MSTPLQGIKVLDFTGVQSGPSCTQMLAWFGADVIKIERPGVGDV
                     TRHQLRDIPDIDALYFTMLNSNKRSIELNTKTAEGKEVMEKLIREADILVENFHPGAI
                     DHMGFTWEHIQEINPRLIFGSIKGFDECSPYVNVKAYENVAQAAGGAASTTGFWDGPP
                     LVSAAALGDSNTGMHLLIGLLAALLHREKTGRGQRVTMSMQDAVLNLCRVKLRDQQRL
                     DKLGYLEEYPQYPNGTFGDAVPRGGNAGGGGQPGWILKCKGWETDPNAYIYFTIQEQN
                     WENTCKAIGKPEWITDPAYSTAHARQPHIFDIFAEIEKYTVTIDKHEAVAYLTQFDIP
                     CAPVLSMKEISLDPSLRQSGSVVEVEQPLRGKYLTVGCPMKFSAFTPDIKAAPLLGEH
                     TAAVLQELGYSDDEIAAMKQNHAI"
     gene            complement(12954..13586)
                     /locus_tag="J1Y83_003399"
     CDS             complement(12954..13586)
                     /locus_tag="J1Y83_003399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfdX family protein"
                     /protein_id="HBA7631515.1"
                     /translation="MKRLIMATMVTAVLASSTVWAADNAPVAAQQQTQQVKQTQKTAA
                     AERISEQGLYAMRDVQVARLALFHGDPEKAKELTNEASALLSDDSTEWAKFAKPGKKT
                     NVNDDQYIVINASVGISESYVATPEKEAAIKIANEKMAKGDKKGAMEELRLAGVGVME
                     NQYLMPLKQTRNALADAQKLLDKKQYYEANLALKGAEDGIIVDSEALFVN"
BASE COUNT         4065 a         3083 c         2792 g         3821 t
ORIGIN      
        1 tccataattt gtgacgatga attaaacacg gggttaaatt gatcgatggt tgctgtcaac
       61 tgactatggt gtaacgattc gcggcgtccc cacagtgtca ttgtcaacga cgtaccaact
      121 gatcctgaca aggtgcgaaa aaaattactc atactcgagg cattggcaaa tttattatct
      181 ggcaagcctg aaaacgaaat cgttgttaag ggtaaaaaga aacaggcaac ggcgaatccc
      241 tgaaaaaact gtggcaaaat gatgcctgta aaatcaatcg ttggcataaa tgtcacagaa
      301 cgccagtaat agcaaaccgc atacatcaaa aaactaaatg tcaccaacaa ccgcatatca
      361 attttgttgc cataacgtcc tatcaaaggt gatattaata gcggcatgat accgatgggc
      421 gcataggcaa gtccggccca tatcgcatta taccccatcg tttcctggag taactgcggc
      481 ataagaacta tcgctccaga gtaaaataaa tacgcgcatg tgatactcac aataccaatg
      541 gtgaaattac gggacttaaa caaactgagg tcaagaatcg gattctctga ggtcgactcc
      601 caaatgacta aagaaatcag agaaataact gatactactg ttagtattat tattgtactc
      661 gagttgaacc aatccagatc gcgcccttta tcaagcataa tttgcaagca accaacaccg
      721 agcactaaca gggtcagtcc tggtagattc attttgaccg gtgaagtctc agtttctctt
      781 cctttaagta aggttaagca taatgtcagg acgataatcc ccatagggac attgattaaa
      841 aatatccaac cccagctaaa gttatcgcaa atatagccgc ccaatatcgg cccacatatc
      901 ggagcgataa ttacggtcat tgaccataat gccagagcaa atgttctttt ttctggtgga
      961 taattcctta ataataaact ctgtgacagt ggaattaacg gccccgccat taacccctga
     1021 acgactctaa aaaatatcag cacatcaaga ttggtcgata aactacacat taatgaagac
     1081 agcgaaaaaa aggttactga aagtaaaaat aaccttaatt cgcctattct ttgtgccagc
     1141 ctgccggtaa cagggatcgc aatggcattt gctacgccaa acgaggtgat aacccaggtg
     1201 ccttcgtctg ttgatgctcc cagaaagcca gatattgtcg gtattgcgac gttagaaata
     1261 gtggaatcca acatttgcat aaacgtcgct aatgacaatg caatagtgac gcaccataac
     1321 gtcccaccgg ttaatggtgc aggagttgat ttagtgattg ccattaaagt tgtccattat
     1381 gcgaaataat gttgctaatt tctttttcta tcggactggt atcgataact aaagccttac
     1441 tggtataagc aggcatggac gtcacggttg aagccagatc gggcatctcg gcaatgtctt
     1501 cattcttcgt atcaatagta gcagtcatcg ataaaccaat gcgcaaaggg tgttccatga
     1561 gttcttttgg atccagagaa acttcaaccg gtacacgctg aacgattttg atccagttcc
     1621 ctgtcgcatt ttgtgcaggt aataaggaga acgcattgcc ggttcccata ttgatccctg
     1681 tcacccgacc atgaaacaca acattttcac cataaagatc gctgataata ttgaccgatt
     1741 gaccaatccg tacatccgtg agttgtgttt ctttaaagtt ggcattaacc cacatttgac
     1801 gcgccggtac gacagccatt aacgattgtc cgggactcac tgtttcgccg acctgaacac
     1861 ttctctgggc aatataaccg gtaaccggac tcttaatatc cgtacgttta agcgccaacc
     1921 aggcttcttt ggttgcatct gcagcttcaa tgacttgtgg ctgacggttt aatggtgtgt
     1981 tcattactaa agctttattc gctttataag cctggatagc agcattcaat gccgctttgc
     2041 tacttattaa cgtatctttg gtatgctcca gcgcttcttt tgaaataacc ccttgcttcg
     2101 ctaaaggcac acgacggtta taatcttcta aagattgttg atactgaata cgtgctgaag
     2161 cgacttcggc actgtattgt ttatcctgta agtatagttt attcgtttgc cgaacaatat
     2221 ttgccagatt atttttagct ttattgagtg cgatagtggc atcagtttta tccagtgaaa
     2281 ctaaaatgtc accttgtcga acgtagttcg tatctttatg attaacgaca gtgacactgc
     2341 ctgagacttg tgcagaaatt ggatctgcat ttcccgtgac ataggcgtca tctgtactaa
     2401 tcatgtcttt taattccatt gaccaatagg cataggcacc tgaaaacgca ataaataaaa
     2461 ctaccgctaa caaagcaaaa tattttcttc tgtcagaatg ttttttattt gaattaatct
     2521 gttccactat tatctctcat ttcgcataga tgaaatttat gaattgaatg aaaagagtat
     2581 tcattcctca tatcaaacac agtgcatgtt ttgcacatta tcccaggaac gttggagggc
     2641 taatttaaat gaagtgcaaa aaaaacaaca gaggactaaa ccgcggcttt tgcaatacaa
     2701 ttcttacgcc tgtaggatta gtgagaaaac ttatagtgct cacttgaaac tataaatcat
     2761 cggtatcatc cctgatttta ttgttgacat tttatttatg ccgactattt atatggtata
     2821 cttgtcgaat tatcttaaag gaagctcaaa ttttcttatt tttattgaga aaatgagatg
     2881 atgccttatg tctgtattac tacagggaga agggaaatgc ttcattgcaa agggaataat
     2941 ctatgaacgc aataattatt gatgaccatc ctcttgctat cgcagcaatt cgtaatttat
     3001 tgatcaaaaa cgatattgaa atcttagcag agttgactga aggcggaagc gccgttcagc
     3061 gggtggaaac acttaagcct gatatcgtca tcattgatgt cgatatcccc ggagttaacg
     3121 gtatccaggt gttagaaacg ctgagaaagc gccaatatag cggaattatt attattgtct
     3181 ccgctaaaaa tgaccatttt tacggaaaac attgtgctga tgctggcgct aatggtttcg
     3241 tgagtaaaaa agaaggcatg aacaatatca ttgcagctat tgaagctgca aaaaatggct
     3301 actgctattt ccccttctct ctcaaccggt ttgttggaag tttaacgtcc gaccagcaaa
     3361 aactcgactc cttatcgaaa caagaaatta gtgtcatgcg gtatattctt gatggcaagg
     3421 ataataatga cattgctgaa aaaatgttca tcagcaacaa aactgtcagc acttataaaa
     3481 gtcgcttgat ggaaaaatta gaatgcaaat cactgatgga tctttacaca ttcgcacaac
     3541 gtaacaaaat cggctaacca catgaagttt ttaccctata tttttcttct ctgttgtggt
     3601 ctttggtcga ccataagttt cgcagacgga gattacatcg aatatcgtgg catcagtagt
     3661 aacaaccgtg tcacacttga tccactacgt ctgagcaaca aggaattacg ttggctcgcg
     3721 agcaaaaaaa atcttgtgat tgcagtacac aagtcccaaa cagctacgtt gttgcatacc
     3781 gactcgcagc aacggattcg tggtattaat gctgattatt taaatctttt aaaaagagcg
     3841 ttaaatatca aattaacact ccgggaatac gcagatcatc aaaaagcaat ggacgcgctg
     3901 gaagacggtg aagttgatat agtgttatca catttagttg cttcgccgcc tcttaatgat
     3961 gacattgctg caactaatcc tctgataatt acctttccgg cgctggtcac tacccttcac
     4021 gattcaatgc gaccgcttac ctcatcaaaa ccagtaaata ttgctcgagt agcaaattat
     4081 cccccagacg aggtaattca tcaatcattt ccaaaagcaa caattatctc ttttacaaat
     4141 ttatatcagg cattagcatc cgtctcagcc ggacagaatg attactttat tggtagtaac
     4201 atcattacca gcagtatgat ttcccgttat tttactcact ccttaaatgt agtgaagtat
     4261 tataactcac cgcgtcaata taacttttta ttaaccagaa aagattcaat cgttcttaat
     4321 gaagtactca atcgatttgt tgatgcttta acaaatgaag ttcgctatga agtatcacaa
     4381 aattggcttg atacaggaaa cctggccttt ctgaacaaac cattagaact cactgaacat
     4441 gaaaaacagt ggattaagca gcatcccgat ttaaaggtgc tggaaaatcc ttactcgcca
     4501 ccctattcta tgacagatga aactggctca gttcgaggcg tgatgggtga tattcttaat
     4561 attattacct tgcaaacagg tttaaatttt tctccgatca ccgtttcaca caatattcat
     4621 gctgggacac agcttaaccc cggtggatgg gatatattgc ccgccgctat ttatagtgaa
     4681 gatcgagaaa ataatgtttc atttgctgaa gtcttcataa caacacctta cgtttttgtc
     4741 atgcaaaaag cgcctgacag tgaacaaaca ttaaaaaaag gaatgaaagt tgccattcca
     4801 tattattatg agcttcattc gcaattaaaa gagatgtatc cggaggtaga gtggataaaa
     4861 gtcgataacg ccagcgctgc atttcacaag gtcaaggaag gtgaacttga tgctctggtc
     4921 gcgacacagt taaattcgcg ttacatgatc gaccattact atcctaatga actttatcat
     4981 tttcttattc ccggcgttca gaatgcatca ctttcgttcg cttttcctcg cggagaaccg
     5041 gaacttaagg atattattaa taaagcactg aatgcaattc ccccaagcga agttctgcgc
     5101 ctgaccgaaa aatggattaa aatgcccaat gtgaccattg acacatggga cctctatagc
     5161 gagcaatttt atattgttac gacattatcc gttttattag ttggcagtag ccttttatgg
     5221 ggattctacc tgttacgctc agttcgtcgt cgtaaagtta ttcagggtga tttagaaaac
     5281 caaatatcat tccggaaagc gctctcggat tccttaccga atccaactta tgttgtaaac
     5341 tggcaaggta atgtcattag tcacaatagt gcatttgaac attatttcac tgctgattac
     5401 tataaaaatg caatgttacc attagaaaat agtgaatccc cctttaaaga tgttttttct
     5461 aatacacatg aagtcacagc agaaacgaaa gaaaaccgaa caatatacac acaggtattt
     5521 gaaattgata atggcatcga gaaaagatgc attaatcact ggcatacctt atgtaatctg
     5581 ccagcaagcg aacatgctgt ttatatttgt ggttggcaag atattaccga gacgcgtgat
     5641 ttaattcatg cactcgaagt agagagaaat aaagcgatca atgcaactgt cgcaaaaagc
     5701 cagtttctgg caacaatgag tcacgaaata agaacaccaa taagctccat tatgggcttc
     5761 ctggaactac tgtcgggttc tggtcttagc aaggaacaac gggtggaggc gatttcactt
     5821 gcctacgcca ccggacaatc actcctcggc ttaattggtg aaatccttga tgtcgacaaa
     5881 attgaatcgg gtaactatca acttcaacca caatgggtcg atatccctac tttagtccag
     5941 aacacttgtc actctttcgg tgcgattgct gcaagcaaat cgatcgcatt aagttgcagc
     6001 agtacgcttc ctgaacgcta cctggttaag atcgaccctc aggcatttaa gcaggtctta
     6061 tctaatttac tgagtaatgc tctcaaattt actaccgagg gggcagtaaa aattacgacc
     6121 tccctgggtc acattgatga caaccacgct gttatcaaaa tgacgattat ggattctgga
     6181 agtggattat ctcaggaaga acaacaacaa ctgtttaaac gctatagcca aacaagtgca
     6241 ggtcgtcagc aaacaggttc tggtttaggc ttaatgatct gcaaagaatt aattaaaaac
     6301 atgcagggcg atttgtcatt agaaagtcat ccaggcatag gaacgatatt tacgatcaca
     6361 atcccggtag aaattaccca acaagtggcg gctgtcgagg caaaagcaga acaacctatc
     6421 acactacctg aaaagttgag catattaatc gcggatgatc atccgaccaa caggctatta
     6481 ctcaaacgcc agctaaatct attaggatat gatgttgatg aagccactga tggtgtgcaa
     6541 gcgctacaca aagtcagtat gcaacattat gatctgctta ttactgacgt taatatgccg
     6601 aatatggatg gttttgagtt gactcgcaaa ctccgtgagc aaaattcttc cttacccatc
     6661 tgggggctta cagccaacgc acaggctaac gaacgtgaaa aagggttaaa ttgcggcatg
     6721 aacttatgtt tgttcaaacc gttgactctg gatgtactga aaacacattt aagtcagtta
     6781 caccaggttg cgcatattgc acctcagtat cgccaccttg atatcgaggc cctgaagaat
     6841 aatacggcga atgatctaca actgatgcag gagattctca tgactttcca gcatgaaacg
     6901 cataaagatc tacccgctgc gtttcatgca ctagaagctg gcgataacag aactttccat
     6961 cagtgtattc atcgcatcca cggtgcggct aacatcctga atttgcaaaa gttgattaat
     7021 attagccacc agttagaaat aacacctgtt tcagatgaca gtaagcctga aattcttcag
     7081 ttgctgaact ctgtaaaaga acacattgca gagctggacc aggagattac tgttttctgt
     7141 cagcaaaatg actaaatagc ggctcccaca atgttcaaat gtgggagcta tttaccagca
     7201 catcttacat ttatgatgag aactcctggc gaatttgttc cccatgctgg tcgagcgttg
     7261 ccgctcccgg cataacatgc gggtccgcgc agccgctgat ttttatcgga ttacccggca
     7321 tcattattcc cccggcttca atcaacatat ttctcgcctg agtttgtggt aaattaatgg
     7381 cctcagccac acttaataac ggcgcgacgg gcacaccaac ttcatgtatt ctggctaacc
     7441 aaacttcagc tacctgcgtt tttaacgtcc gctcaatata ttctttaaga atagcctggt
     7501 tatgtacgcg taaaatattg ctgctaaatc ggggatcatt aaccagttcc gtaagctcca
     7561 gtgcctggca taacgcagaa aaaagcttgt cattaccaca acaaatcgta atcggcttat
     7621 cctgagtatc gaaaacatca aaaggtgcca tgtagggatg gcgatttcct aaacgttgtg
     7681 gtgacttccc agtcgcgata tatgccatca gtccatgctc cagaaaactc agagtggcat
     7741 caaacatcgc tatatcgaca tgcgcccctc tctggctctt ttcgcggcca taaagtgcac
     7801 tcactattcc actgaataaa taaacaccgc cacagagatc cgcaagagag gtaccaacgc
     7861 gcactggcgg agcatcaggg tatcctgttt ccatcattat cccgctcatt gcctgaatga
     7921 tggtatcgta ggcaggagca tcttttagcg gaccggtatg tccgaaacct gacgatgaag
     7981 catatataag gcgcgggttg atttcttgta acctttccca tgaaaacccg agtttttcca
     8041 ttgtacctgg acgaaaattc tcagctaata catcagcttg tttaagcata tttataaata
     8101 tacttttatc gtgatcattc tttaaatcaa gaaccacact ctctttgcca tgattaataa
     8161 aactgtaata gagtgactgt ccatccacat agggaccaaa tgtgcgggta tcatcaccat
     8221 gacccggcgg ctcaacttta attacccttg cacccatatt acaaagaagt tgagttccga
     8281 aaggcccatt aaggacatgc gtcatatcga taactaataa gccttcaaac ggccctttgc
     8341 attcattatt tgtcatttgc ttatgccttg aaaaaagccg aaggtgaaaa gacacttcgg
     8401 catttatgta aaatcaataa catgattaat tatgaaacca gtcgactgac ataaatccac
     8461 aaaggagccg tgacgacaaa acccagtacg ctcacagcca atgacgctgt accagtgcgg
     8521 gtgtagacat taaaccggct ggcaataatg attccggaga atgccggcgg taatgcgcct
     8581 gccagtacca tcatctgcaa atgttcgcta ttcaaatgac atgccatacc gacgagaagc
     8641 agcgccagtg gcatcagaat cagcttcagg aaagtgttat aagcaatttc ggcactgaac
     8701 tcgaatttat gtgcagccag agtcaacccc gcagcgaata ccgctacccc tgagttagcc
     8761 ttcgcaatca gattaaaggt tgggtcccat gctgccggaa ttcttacccc aaccaatacc
     8821 aggatcgttg ccagaacagg agcccatact actggctcct ttgccgcaga aattaatgcg
     8881 ctcagattac tattcttctt accatccgct cctgaggaag gattcagcaa atacagacca
     8941 ataggaatag taattgcgtt aacaataata gaaataattg ctaccactaa acctgttgat
     9001 acggaatcac cataaatagg atcgagaact gcaaacccca ggaatccaat ggtaggtgaa
     9061 cctgcaatta atgcacatac tgctgcttct gcatgggtac gtttaaaaaa tttgtagcaa
     9121 ccgaaccagg agaaaaagaa acacccgaca ataaccacaa gtgatacaag ggtcaggcga
     9181 gtgtccgcaa aaatcatttc cctgtttgcc ctggtaatag aaacaaatag agccgcagga
     9241 agagcgtagt ttaataccag tttattaaat gcccgagctt gatcctctga aaaagtttct
     9301 cgtctgccgc taaaataacc caacagcatg attacgataa taggcaataa atcgccaata
     9361 aaaaatgtta gcataatagt atttccgata agggtcccct taccatcgca taataagttg
     9421 gcaagaggag acgatgtcag tatttaatta ccagcgactt gttttgggtt aagtttggta
     9481 atatggccac tttcagttcc tgctgctggg tcgatgacca cattaataat ggtcggtttg
     9541 cgcgactgaa ttccggtggt taaagcatga cgaagttcat ctgtcgtggt gacgttatag
     9601 ccaacgccac gaaacgcatc cattaatttg tcataccttg catggtgcaa cagatccgtt
     9661 ggcgatggtg caccagcgcc gctgaggtca acaccgtctc ctctgtagat gccgccatta
     9721 ttaaaaataa cgatcgtcac cggcaggtta tatcgacaaa tcgtttcaat ctccatccca
     9781 ctgaaaccaa aagcactatc accttcaatg gcgacaaccg gagaaccaga ggtcacgcta
     9841 gcaccgatgg catagcccat accgatgccc atgacacccc aggtaccaca atccagacga
     9901 cgacgtggtt tatacatatc aataatattt cgtgcattat ccagggtatt tgccccttca
     9961 ttaactaaat aaatatcctg gttctcgcgc aaaacatcgc gcaccgcact caatgcatta
    10021 aagtaattta atggttgggt atctgtactt aacttttcat gcattttttg tgcattttgc
    10081 tgcttgtgga tatttaaaat atcgcgccac accagtggag tcgtgaatgt gttttgtttc
    10141 aattctgcca gcataccttg catactggat gcaatatcgc caacgactgg cacagcaatg
    10201 gggcggttgc tgtcaatttc ctgcggttca atatccagtt gaataaactg tgtatctgcc
    10261 gcccatcctt ttttaccgtg tgccagtaac caattcagtc gtgcaccaac aagcatgaca
    10321 acgtcagcat ttgccagggc aaacgaacgc gcagctgccg cagaaagtgg atgcgtatct
    10381 tcgaggatcc ctttcgccat agacattggc aggaatggaa tctgggcact ttcaataaat
    10441 tcacgaagct gttcatcaga ttgtgaatac gccgcgcctt tgccaaggat aattaatggt
    10501 cgttcagctt ttgctaaaag cgaaattgcg ctagtgactg acttcgggca tggcaataat
    10561 gctggcgacg gattttcaac tttaacaatc gtggttaacg cttcgtcttt ttccatcgtc
    10621 gcggccagga catttgctgg caaatcaaga taaacaccgc caggacgacc cgatactgag
    10681 acccggatag cgcgtgccaa tgcaatgcca agatcctgcg gctgattaac gcgaaatgct
    10741 gctttggcat acggttttgc cgcattcatt tggtccagct cttcataatc accttgctgg
    10801 agatcgacga tcgcgcggtc gctggagccg ctaatcatga tcatcggaaa accatttacc
    10861 gttgcattgg ccaatgcggt caaaccattg aggaatcctg gcgcagacac tgtcaggcag
    10921 atccctggtt tttgggtaag aaaaccgctt gctgcagcgg catagcctgc cgactgctca
    10981 tgacgaaaac caatataacg aatgccttcc gcctgggcat ggcgcgccat atccgtcaca
    11041 ggaataccta caacaccata aatagtgtca atattattct gttttaatgc ttcaacgatg
    11101 atatgcatac catctgtcat ttgaagttga tctgacatga ttaataacct cgaaattttt
    11161 ataaattggg agccagaagc ccccgttaat atcagatggc gtggttttgc ttcattgcag
    11221 caatttcatc gtcgctataa cccagctcct gcaatacagc agcggtatgt tcacctaata
    11281 gcggcgcagc tttaatatcc ggcgtaaagg cagagaattt cattggacaa ccaactgtca
    11341 gatattttcc acgcaacggt tgttccactt cgacaacact gccactttgg cgcaaagagg
    11401 gatcaagtga aatttctttc atacttaaaa ccggtgcaca aggaatatca aactgagtca
    11461 aataggccac cgcttcatgt ttatcaatag tgacagtgta tttttcgatt tcagcaaaaa
    11521 tatcgaaaat atgtggctgt cgggcatggg ctgtactgta tgccggatcg gtaatccatt
    11581 ctggtttgcc gatggcttta caggtgtttt cccagttttg ctcctgaata gtgaaataaa
    11641 tataggcgtt aggatcggtt tcccagcctt tacatttcag gatccagcca ggctgaccgc
    11701 caccacccgc attaccaccg cggggaactg catcaccaaa tgtgccattc ggatactgcg
    11761 ggtattcttc cagatacccc aacttatcga gacgctgctg gtcgcgtaat ttcacgcggc
    11821 aaaggttcaa cacggcatcc tgcattgaca tggtgactcg ttgcccacgc cccgtttttt
    11881 cgcgatgcag caaagcagca agtaaaccga tcagcaaatg cattcctgtg ttgctgtcac
    11941 ccaacgctgc agcgcttacc agcggcggac cgtcccaaaa acctgtagta gatgccgcgc
    12001 cacccgctgc ctgagcaacg ttttcatagg cttttacatt cacataaggc gaacactcat
    12061 caaacccttt gatcgaacca aaaatcagac gtggattgat ttcttgaata tgctcccagg
    12121 tgaagcccat atgatcaatg gcccctggat gaaagttctc gactaagata tcagcttcgc
    12181 ggatcagctt ttccattacc tctttgcctt ccgctgtttt ggtatttaat tcaatagaac
    12241 gtttgttact gttaagcatg gtgaagtaaa gcgcatcgat atcaggaata tctcgcagct
    12301 ggtggcgcgt tacgtcacca acgccgggac gttcaatttt aatgacgtca gcgccaaacc
    12361 aggccagcat ttgagtacaa gatgggccag attgcacacc ggtgaaatcg agaactttaa
    12421 ttccttgaag tggagttgac atagcaatac ctattagtta attaaatatg tgttaattta
    12481 gcgtttattt aaggcataac gcctctataa ataatctgca aaatatcgtc gtagctgcac
    12541 tcgatatttt taaattcctg tatcaagtgc tgaaaatcat ttcatcgcta cttccaattc
    12601 ctttctgcat ggtaagtagt gaggacgaaa agaagaatat aataaatatt ggttcgcggt
    12661 aatcagagag agccgcgaca tttgtgatgt tattcttaca actgtaagaa gtgtcagtga
    12721 tgtacaagta agggtatgat tacactattt aaagcgtacc ataaaatata acataataaa
    12781 tgatttgtga taatatggga ttattgcggt ttactttgag gaagataaaa tgcgagttta
    12841 tagaaccgcg agcgccaaat aattttatga ccacaacctg tcgtattgtg gtcataaaaa
    12901 attgtcggct ggagggagtt aaatccttta cctcaatata ctgcctgtca gcgttagttc
    12961 acaaacagtg cttcgctatc gacgatgatt ccatcttccg cacctttaag cgccaggttt
    13021 gcctcgtagt attgcttttt atcaagtagt ttctgcgcat cagcaagtgc attacgagtc
    13081 tgtttgagcg gcatcagata ttggttttcc attacgccaa caccggctag gcgaagctct
    13141 tccattgcgc cctttttatc ccctttcgcc attttttcgt tggcaatttt tatcgcggct
    13201 tctttttcgg gtgtcgctac ataactctca gaaattccga cagatgcatt aatgacaata
    13261 tattggtcat cattgacatt ggtcttttta cctggctttg cgaactttgc ccattcggta
    13321 ctatcatcag acagcaaagc ggaagcttca ttggtcagtt cttttgcctt ttccggatcg
    13381 ccatgaaaca gagccaggcg cgctacctgc acgtcacgca tcgcatataa accttgttca
    13441 gagatacgct ctgctgctgc cgttttctgg gtttgcttaa cttgctgagt ttgctgttgt
    13501 gccgctaccg gtgcattatc agcagcccat acagttgaag atgccagaac tgctgtgacc
    13561 atcgtagcca taattaaacg tttcatactt acctcattct ttaacagttt tagaaaacgc
    13621 cgccatcata ttgggtgagc atgaaatgag aaatcagaga gcaacggaca ttttgaaagg
    13681 gttatcttac aactgtagtt attatcgcaa agccgctttt agtcttacag ttgtaggaat
    13741 ctgcttcctg tcgcagagaa c
//
LOCUS       DADSYM010000130        13735 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2415
            SAMN08039579-rid11723173.denovo.131, whole genome shotgun sequence.
ACCESSION   DADSYM010000130 DADSYM010000000
VERSION     DADSYM010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039579
            Sequence Read Archive: SRR6362533
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13735)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13735)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980162.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 03:39:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,484
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 4,347
            CDSs (with protein)               :: 4,347
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 43 of 85
            Pseudo Genes (incomplete)         :: 37 of 85
            Pseudo Genes (internal stop)      :: 19 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13735
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039579-rid11723173.denovo.131"
                     /strain="LGE2415"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8302 N 95.9628 W"
                     /collection_date="2015-08-24"
     gene            complement(<1..248)
                     /locus_tag="J1Y83_003400"
     CDS             complement(<1..248)
                     /locus_tag="J1Y83_003400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA7631516.1"
                     /translation="MSENDIIIRTHYESPLRMHIDSDILTPSSEPINQFARQLITLLD
                     TSYLSSMLSYCVTQEFTAICRKISQNCYSTALFIINFAT"
     gene            772..2700
                     /gene="thrS"
                     /locus_tag="J1Y83_003401"
     CDS             772..2700
                     /gene="thrS"
                     /locus_tag="J1Y83_003401"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010245687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HBA7631517.1"
                     /translation="MPVITLPDGSQRHYDHAVSPMDVALDIGPGLAKACIAGRVNGEL
                     VDACDLIENDAQLSIITAKDEEGLEIIRHSCAHLLGHAIKQLWPHTKMAIGPVIDNGF
                     YYDVDLDRTLTQEDVEALEKRMHELAEKNYDVIKKKVSWHEARETFANRGESYKVSIL
                     DENIAHDDKPGLYFHEEYVDMCRGPHVPNMRFCHHFKLMKTAGAYWRGDSNNKMLQRI
                     YGTAWADKKALNAYLQRLEEAAKRDHRKIGKQLDLYHMQEEAPGMVFWHNDGWTIFRE
                     LEVFVRSKLKEYQYQEVKGPFMMDRVLWEKTGHWDNYKDAMFTTSSENREYCIKPMNC
                     PGHVQIFNQGLKSYRDLPLRMAEFGSCHRNEPSGSLHGLMRVRGFTQDDAHIFCTEEQ
                     IRDEVNGCIRLVYDMYSTFGFEKIVVKLSTRPEKRIGSDEMWDRAEADLAVALEENNI
                     PFEYQLGEGAFYGPKIEFTLYDCLDRAWQCGTVQLDFSLPSRLSASYVGEDNERKVPV
                     MIHRAILGSMERFIGILTEEFAGFFPTWLAPVQVVIMNITDSQSEYVNELTQKLSNAG
                     IRVKADLRNEKIGFKIREHTLRRVPYMLVCGDKEVESGKVAVRTRRGKDLGSMDVNEV
                     IEKLQQEIRSRSLKQLEE"
     gene            2704..3246
                     /gene="infC"
                     /locus_tag="J1Y83_003402"
     CDS             2704..3246
                     /gene="infC"
                     /locus_tag="J1Y83_003402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HBA7631518.1"
                     /translation="MKGGKRVQTARPNRINGEIRAQEVRLTGLEGEQLGIVSLREALE
                     KAEEAGVDLVEISPNAEPPVCRIMDYGKFLYEKSKSSKEQKKKQKVIQVKEIKFRPGT
                     DEGDYQVKLRSLIRFLEEGDKAKITLRFRGREMAHQQIGMEVLNRVKDDLQELAVVES
                     FPTKIEGRQMIMVLAPKKKQ"
     gene            3343..3540
                     /gene="rpmI"
                     /locus_tag="J1Y83_003403"
     CDS             3343..3540
                     /gene="rpmI"
                     /locus_tag="J1Y83_003403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006708231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HBA7631519.1"
                     /translation="MPKIKTVRGAAKRFKKTGKGGFKHKHANLRHILTKKATKRKRHL
                     RPKAMVSKGDLGLVIACLPYA"
     gene            3593..3949
                     /gene="rplT"
                     /locus_tag="J1Y83_003404"
     CDS             3593..3949
                     /gene="rplT"
                     /locus_tag="J1Y83_003404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016169860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HBA7631520.1"
                     /translation="MARVKRGVIARARHKKILKQAKGYYGARSRVYRVAFQAVIKAGQ
                     YAYRDRRQRKRQFRQLWIARINAAARQNGISYSKFINGLKKASVEIDRKILADIAVFD
                     KVAFTALVEKAKAALA"
     gene            4072..4116
                     /locus_tag="J1Y83_003405"
     CDS             4072..4116
                     /locus_tag="J1Y83_003405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanyl--tRNA ligase operon leader peptide"
                     /protein_id="HBA7631521.1"
                     /translation="MNAAIFRFFFYFST"
     gene            4400..5383
                     /gene="pheS"
                     /locus_tag="J1Y83_003406"
     CDS             4400..5383
                     /gene="pheS"
                     /locus_tag="J1Y83_003406"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004851795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HBA7631522.1"
                     /translation="MSHLAELVASAKAAISQASDVAALDNVRVEYLGKKGHLTLQMTT
                     LRELPPEERPAAGAVINEAKEQVQQALNTRKAELESAALNARLAAETIDVSLPGRRIE
                     NGGLHPVTRTIDRIESFFGELGFTVATGPEIEDDYHNFDALNIPGHHPARADHDTFWF
                     DATRLLRTQTSGVQIRTMKAQQPPIRIIAPGRVYRNDYDQTHTPMFHQMEGLIVDTNI
                     SFTNLKGTLHDFLRNFFEEDLQIRFRPSYFPFTEPSAEVDVMGKNGKWLEVLGCGMVH
                     PNVLRNVGIDPEVYSGFAFGMGMERLTMLRYGVTDLRSFFENDLRFLKQFK"
     gene            5398..7785
                     /gene="pheT"
                     /locus_tag="J1Y83_003407"
     CDS             5398..7785
                     /gene="pheT"
                     /locus_tag="J1Y83_003407"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012905611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HBA7631523.1"
                     /translation="MKFSELWLREWVNPAIDSDALANQITMAGLEVDGVEPVAGSFHG
                     VVVGEVVECAQHPNADKLRVTKVNVGGDRLLDIVCGAPNCRQGLRVAVATIGAVLPGD
                     FKIKAAKLRGEPSEGMLCSFSELGISDDHNGIIELPADAPIGTDIREYLKLDDNTIEI
                     SVTPNRADCLGIIGVARDVAVLNQLPLVEPEIVPVGATIDDTLPIAVEAPDACPRYLG
                     RVVKGINVKAPTPLWMKEKLRRCGIRSIDAVVDVTNYVLLELGQPMHAFDKDRIEGGI
                     VVRMAKEGETLVLLDGTEAKLNADTLVIADHNKALAMGGIFGGEHSGVNDETQNVLLE
                     CAFFSPLSITGRARRHGLHTDASHRYERGVDPALQHKAMERATRLLIDICGGEAGPVI
                     DITNEATLPKRATITLRRSKLDRLIGHHIADEQVTDILRRLGCEVTEGKDEWQAVAPS
                     WRFDMEIEEDLVEEVARVYGYNNIPDEPVQASLIMGTHREADLSLKRVKTLLNDKGYQ
                     EVITYSFVDPKVQQMIHPGVEALLLPSPISVEMSAMRLSLWTGLLATVVYNQNRQQNR
                     VRIFESGLRFVPDTQAPLGIRQDLMLAGVICGNRYEEHWNLAKETVDFYDLKGDLESV
                     LDLTGKLNEVEFRAEANPALHPGQSAAIYLKGERIGFVGVVHPELERKLDLNGRTLVF
                     ELEWNKLADRVVPQAREISRFPANRRDIAVVVAENVPAADILSECKKVGVNQVVGVNL
                     FDVYRGKGVAEGYKSLAISLILQDTSRTLEEEEIAATVAKCVEALKERFQASLRD"
     gene            7790..8089
                     /gene="ihfA"
                     /locus_tag="J1Y83_003408"
     CDS             7790..8089
                     /gene="ihfA"
                     /locus_tag="J1Y83_003408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit alpha"
                     /protein_id="HBA7631524.1"
                     /translation="MALTKAEMSEYLFDKLGLSKRDAKELVELFFEEIRRALENGEQV
                     KLSGFGNFDLRDKNQRPGRNPKTGEDIPITARRVVTFRPGQKLKSRVENASPKDE"
     gene            8190..9170
                     /gene="btuC"
                     /locus_tag="J1Y83_003409"
     CDS             8190..9170
                     /gene="btuC"
                     /locus_tag="J1Y83_003409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter permease BtuC"
                     /protein_id="HBA7631525.1"
                     /translation="MLTLARQQQRQNIRWLLCLSVLMLLALLLSLCAGEQWILPGDWF
                     SPRGELFVWQIRLPRTLAVLLVGAALAISGAVMQALFENPLAEPGLLGVSNGAGVGLI
                     AAVLLGQGQLPNWALGLCAIAGALIITLILLRFARRHLSTSRLLLAGVALGIICSALM
                     TWAIYFSTSVDLRQLMYWMMGGFGGVDWRQSWLMLALIPVLLWICCQSRPMNILALGE
                     ISARQLGLPLWFWRNVLVAATGWMVGVSVALAGAIGFIGLVIPHILRLCGLTDHRVLL
                     PGCALAGASALLLADIVARLALAAAELPIGVVTATLGAPVFIWLLLKAGR"
     gene            9232..9783
                     /gene="btuE"
                     /locus_tag="J1Y83_003410"
     CDS             9232..9783
                     /gene="btuE"
                     /locus_tag="J1Y83_003410"
                     /EC_number="1.11.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional thioredoxin/glutathione peroxidase"
                     /protein_id="HBA7631526.1"
                     /translation="MQDSILMTVVKDIDGEVTTLEKYAGNVLLIVNVASKCGLTPQYE
                     QLENIQKAWADRGFVVLGFPCNQFLEQEPGSDEEIKTYCTTTWGVTFPMFSKIEVNGE
                     GRHPLYQKLIAAAPTAVAPEESGFYARMVSKGRAPLYPDDILWNFEKFLVGRDGLVIQ
                     RFSPDMTPEDPIVMESIKLALAK"
     gene            9783..10532
                     /gene="btuD"
                     /locus_tag="J1Y83_003411"
     CDS             9783..10532
                     /gene="btuD"
                     /locus_tag="J1Y83_003411"
                     /EC_number="7.6.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter ATP-binding protein
                     BtuD"
                     /protein_id="HBA7631527.1"
                     /translation="MSIVMQLQDVAESTRLGPLSGEVRAGEILHLVGPNGAGKSTLLA
                     RMAGMTSGKGSIQFAGQPLEAWSATKLALHRAYLSQQQTPPFAMPVWHYLTLHQHDKT
                     RTELLNDVAGALALDDKLGRSTNQLSGGEWQRVRLAAVVLQITPQANPAGQLLLLDEP
                     MNSLDVAQQSALDKILSALCQQGLAIVMSSHDLNHTLRHAHRAWLLKGGKMLASGRRE
                     EVLTPANLAQAYGMNFRRLDIEGHRMLISTI"
     gene            10610..11074
                     /locus_tag="J1Y83_003412"
     CDS             10610..11074
                     /locus_tag="J1Y83_003412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase"
                     /protein_id="HBA7631528.1"
                     /translation="MRFCLILITALFLAGCSHHKAPPPNARLSDSITVIAGLNDQLQS
                     WHGTPYRYGGMTRRGVDCSGFVVVTMRDRFDLQLPRETKQQASIGTQIDKDELLPGDL
                     VFFKTGSGQNGLHVGIYDTNNQFIHASTSKGVMRSSLDNVYWQKNFWQARRI"
     gene            11321..12034
                     /gene="ydiV"
                     /locus_tag="J1Y83_003413"
     CDS             11321..12034
                     /gene="ydiV"
                     /locus_tag="J1Y83_003413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-FlhDC factor"
                     /protein_id="HBA7631529.1"
                     /translation="MKIFLENLYHSDCYFLPIRDNQQDLVGVELITHFSSEDGTVRIP
                     TSRVIAQLTEEQHWQLFSEQLELLKSCQHFFIQHKLFAWLNLTPQVATLLLDRDNFAG
                     ELLKYPFIELLINENYPHFNEGKDNRDLLSLSQMYPLVLGNLGAGNSTMKAVFDGLFT
                     RVMLDKSFIQQQITHRSFEPFIRAIQAQISPCCNCIIAGGIDTPEILAQIIPFDFHAL
                     QGCLWPAVPINQITTLVQR"
     gene            12097..13533
                     /locus_tag="J1Y83_003414"
     CDS             12097..13533
                     /locus_tag="J1Y83_003414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiU family protein"
                     /protein_id="HBA7631530.1"
                     /translation="MTLSFITRWCDELPETYTALSPTPLNNARLIWHNTELANTLSIP
                     SSLFKNGAGVWGGETLLPGMSPLAQVYSGHQFGVWAGQLGDGRGILLGEQLLADGTTM
                     DWHLKGAGLTPYSRMGDGRAVLRSTIRESLASEAMHYLGIPTTRALSIVTSDSPVYRE
                     TVEPGAMLMRVAPSHLRFGHFEHFYYRREPEKVRQLADFAIRHYWSHLDDEEDKYRLW
                     FTDVVARTASLIAQWQTVGFAHGVMNTDNMSLLGLTLDYGPFGFLDDYEPGFICNHSD
                     HQGRYSFDNQPAVALWNLQRLAQTLSPFVAVDGLNEALDSYQQVLLTHYGQRMRQKLG
                     FMTEQKEDNALLNELFSLMARERSDYTRTFRMLSLTEQHSAASLLRDEFIDRAAFDDW
                     FARYRVRLQQDEVTDSERQQLMQSVNPALVLRNWLAQRAIEASEKGDMTELHRLHEAL
                     RNPFSDRDDDYVSRPPDWGKRLEVSCSS"
     gene            complement(13537..13728)
                     /gene="hemP"
                     /locus_tag="J1Y83_003415"
     CDS             complement(13537..13728)
                     /gene="hemP"
                     /locus_tag="J1Y83_003415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemin uptake protein HemP"
                     /protein_id="HBA7631531.1"
                     /translation="MRYTDSRKLTPETDASHKTASPQPIRRITSQTLLGPDGKLIIDH
                     DGQEYLLRKTQAGKLLLTK"
BASE COUNT         3442 a         3191 c         3675 g         3427 t
ORIGIN      
        1 gtggcaaagt taatgataaa aagggcagtg gaataacaat tttgtgaaat ttttcgacag
       61 attgcggtaa attcctgagt aacacagtat gacagcatcg aacttaaata agaggtatca
      121 agtagggtga tgagctggcg cgcaaattga ttaataggct ctgatgaagg cgttagtatg
      181 tcgctatcga tgtgcattct aagaggagac tcataatgag ttctgataat gatatcgttt
      241 tcactcataa tctattcgca ttatatatgt ctggaggttt tgagtatgat gattaattat
      301 tattgccaga acgaaatgcg cacgaaaaat tgctataata attgtgtcac ttttatcatt
      361 gcgatatgac cacttcgtca ctgaatctca tgatcgtgta aataccaccg ccaaataacg
      421 caacgaactt tgctggattc agtttatgct gctgtaaatc cgctcgcagt aaacctttca
      481 gaagctctgt gatgttatca gtggttcttt aagcgttttg ctggtgtact cactacaaac
      541 gaattgcgaa tcgaatcaat gtgaaacgga aaggtacaat ctcccttttt ttagtgttaa
      601 catcgttcaa ccgggtggaa ggtgagaggt taatgtcttt catttgtgta cctgattgcg
      661 aaccaattta gcatttgttg gctagatggt ttcgcaatga actgttaata aacaaatttt
      721 tctttgtatg tgatctttcg tgtgggtcac cactgcaaat aaggatataa aatgcctgtt
      781 ataactcttc ctgatggcag ccaacgccat tacgaccacg ctgtaagccc catggatgtt
      841 gcgctggaca ttggtccagg tctggcgaaa gcctgtatcg cagggcgcgt taatggcgaa
      901 ctggttgatg cttgcgatct gattgaaaac gacgcacaac tgtcgatcat tactgccaaa
      961 gacgaagaag gtctggagat cattcgtcac tcctgtgcgc acctgttagg gcacgcgatt
     1021 aaacaacttt ggccgcatac caaaatggca atcggcccgg ttattgacaa cggtttttat
     1081 tacgacgttg atcttgaccg cacgttaacc caggaagatg tcgaagcact cgagaagcgg
     1141 atgcatgagc ttgctgagaa aaactacgac gtcattaaga agaaagtcag ctggcatgaa
     1201 gcgcgtgaaa ctttcgccaa ccgtggggag agctacaaag tctccattct tgacgaaaac
     1261 atcgcccatg atgacaagcc aggtctgtac ttccatgaag aatatgtcga tatgtgccgc
     1321 ggtccgcacg taccgaacat gcgtttctgc catcatttca aactaatgaa aacggcaggg
     1381 gcttactggc gtggcgacag caataacaaa atgttgcaac gtatttacgg tacggcgtgg
     1441 gcagacaaaa aagcgcttaa cgcttacctg caacgcctgg aagaagccgc gaaacgcgac
     1501 caccgtaaaa tcggtaaaca gctcgacctg taccatatgc aggaagaagc gccgggtatg
     1561 gtattctggc acaacgacgg ctggaccatc ttccgtgaac tggaagtgtt tgttcgttct
     1621 aaactgaaag agtaccagta tcaggaagtt aaaggtccgt tcatgatgga ccgtgtcctg
     1681 tgggaaaaaa ccggtcactg ggacaactac aaagatgcaa tgttcaccac gtcttctgag
     1741 aaccgtgaat actgcattaa gccgatgaac tgcccggggc acgtacaaat tttcaaccag
     1801 gggctgaagt cttatcgcga tctgccgctg cgtatggccg agtttggtag ctgccaccgt
     1861 aacgagccgt caggttcgct gcatggcctg atgcgcgtgc gtggttttac ccaggatgac
     1921 gcgcatatct tctgtactga agaacaaatt cgcgatgaag ttaacggatg tatccgttta
     1981 gtctatgata tgtacagcac ttttggcttc gagaagatcg tcgtcaaact ctccactcgt
     2041 cctgaaaaac gtattggcag cgacgaaatg tgggatcgtg ctgaggcgga cctggcggtt
     2101 gcgctggaag aaaacaacat cccgtttgaa tatcaactgg gtgaaggcgc tttctacggt
     2161 ccgaaaattg aatttaccct gtatgactgc ctcgatcgtg catggcagtg cggtacagta
     2221 cagctggact tctctttgcc ttctcgtctg agcgcttctt atgtaggcga agacaatgaa
     2281 cgtaaagtac cggtaatgat tcaccgcgca attctggggt cgatggaacg tttcatcggt
     2341 atcctgaccg aagaatttgc tggtttcttc ccgacctggc ttgcgccagt tcaggttgtt
     2401 atcatgaata ttaccgattc tcagtctgaa tacgttaacg aactgacgca aaaactatca
     2461 aatgcgggca ttcgtgttaa agcagacttg agaaatgaga agattggctt taaaatccgc
     2521 gagcacactt tgcgtcgcgt cccatatatg ctggtctgtg gtgataaaga ggtggaatca
     2581 ggcaaagttg ccgttcgcac ccgccgtggt aaagacctgg gaagcatgga cgtaaatgaa
     2641 gtgatcgaga agctgcaaca agagattcgc agccgcagtc ttaaacaatt ggaggaataa
     2701 ggtattaaag gcggaaaacg agttcaaacg gcgcgcccta accgtatcaa tggcgaaatt
     2761 cgcgcccagg aagttcgctt aacaggtctg gaaggcgagc agcttggtat tgtgagtctg
     2821 agagaagctc tggagaaagc agaagaagcc ggagtagact tagtcgagat cagccctaac
     2881 gccgagccgc cggtttgtcg tataatggat tacggcaaat tcctctatga aaagagcaag
     2941 tcttctaagg aacagaagaa aaagcaaaaa gttatccagg ttaaggaaat taaattccgt
     3001 cctggtacag atgaaggcga ctatcaggta aaactccgca gcctgattcg ctttctcgaa
     3061 gagggtgata aagccaaaat cacgctgcgt ttccgcggtc gtgagatggc gcaccagcaa
     3121 atcggtatgg aagtgcttaa tcgcgtgaaa gacgatttgc aagaactggc agtggtcgaa
     3181 tccttcccaa cgaagatcga aggccgccag atgatcatgg tgctcgctcc taagaagaaa
     3241 cagtaaggcc ttcaagtagc actgtctgtg gagcctttgg gcttcacagg cattgttcgc
     3301 ctttgtttcg tttattaaca atgcgaagtg gaagttatta aaatgccaaa aattaagacc
     3361 gtacgcggtg ctgctaagcg cttcaaaaaa accggtaaag gtggttttaa gcacaagcac
     3421 gctaacctgc gtcacattct gaccaaaaaa gcgaccaaac gtaaacgtca cctgcgtccg
     3481 aaagccatgg tttccaaagg cgatctgggc ctggtaatcg cgtgcctgcc gtacgcataa
     3541 gccgttaacg tttttaactt tttaattaga atatagatac aggagagcac atatggctcg
     3601 cgtaaaacgt ggtgttattg cacgcgcacg tcacaagaaa attttgaaac aagctaaagg
     3661 ctactacggt gcgcgttctc gcgtataccg cgttgccttc caggctgtta tcaaagctgg
     3721 tcagtatgct taccgcgacc gtcgtcaacg taagcgtcag ttccgtcaac tgtggattgc
     3781 gcgtatcaac gcagcagcac gtcagaacgg tatttcttac agcaaattca tcaatggcct
     3841 gaaaaaagcc tctgttgaaa tcgaccgtaa gatcctggct gatatcgcag tattcgacaa
     3901 agtagcgttc accgctctgg ttgaaaaagc gaaagcagct ctggcataag ccagttgaaa
     3961 gagggagcta gtctccctct tttcgtttca acgccatcaa aacattgact tttatcgccg
     4021 tagccttttc aataaaggtc ttttgaagag taccaaaagg taacgcaagc aatgaatgct
     4081 gctattttcc gcttcttttt ttactttagc acctgactcc aggaggctag cgcgtgagaa
     4141 gagaaacgga aaacagcgcc tgaaagcctc ccagtggagg ctttttttgt atgcgcgttt
     4201 tgaaaaataa aaagcctgtc tcatcaggcg taattgtcgc agccagtctg gacacggcca
     4261 gcgcggagaa accggagcgt acttaagtac gtgaggattt cgagcactgc ccgggaccaa
     4321 aatggcaagt aaaatagcct gatgggatgg gctctaagtc caacgaacca gtgtcaccac
     4381 tgacacaatg aggaaaaaca tgtcacatct cgcagaactg gttgccagtg cgaaggcggc
     4441 cattagccag gcgtcagatg ttgccgcgtt agataatgtg cgcgtcgaat atttgggtaa
     4501 aaaagggcac ttaacccttc agatgacgac cctgcgtgag ctgccgccag aagagcgtcc
     4561 ggcagctggt gcggttatca acgaagcgaa agagcaggtt cagcaggcgc tgaatacgcg
     4621 taaagcggaa ctggaaagcg cagcactgaa tgcgcgtctg gcggcggaaa cgattgatgt
     4681 ctccctgccg ggtcgtcgca ttgaaaacgg cgggctgcat ccggtgaccc gtaccatcga
     4741 ccgtatcgaa agtttcttcg gtgagcttgg ctttaccgtg gcaaccgggc cggaaatcga
     4801 agacgattat cataacttcg atgctctgaa cattcctggt caccacccgg cgcgcgctga
     4861 ccacgacact ttttggtttg acgctacccg cctgctgcgt acccagacct ctggcgtaca
     4921 gatccgcacc atgaaagccc agcagccacc gattcgtatc atcgcgcctg gccgcgttta
     4981 tcgtaacgac tacgaccaga ctcacacgcc gatgttccat cagatggaag gtctgattgt
     5041 tgataccaac atcagcttta ccaacctgaa aggcacgctg cacgacttcc tgcgtaactt
     5101 ctttgaggaa gatttgcaga tccgcttccg tccttcctac ttcccgttta ccgaaccttc
     5161 tgcggaagtg gacgtcatgg ggaaaaacgg taaatggctg gaagtactgg gctgcgggat
     5221 ggtgcatccg aacgtgctgc gtaacgttgg catcgacccg gaagtttact ctggtttcgc
     5281 cttcgggatg gggatggagc gtctgaccat gttgcgttac ggcgtcaccg acctgcgttc
     5341 attcttcgaa aacgatctgc gtttcctcaa acagtttaaa taaggcagga atagattatg
     5401 aaattcagtg aactgtggtt acgcgaatgg gtgaacccgg cgattgatag cgacgcgctg
     5461 gcaaatcaaa tcacgatggc gggcctggaa gttgatggtg tagaaccggt tgccggtagc
     5521 ttccacggcg tggtcgttgg tgaagtggtt gagtgtgcgc agcatccgaa cgctgacaaa
     5581 ctgcgtgtga caaaagtgaa tgtcggcggc gatcgcctgc tggacatcgt ctgcggtgcg
     5641 ccaaactgcc gtcagggcct gcgtgtggcg gtagcgacca ttggtgctgt tctgccgggt
     5701 gatttcaaaa ttaaagccgc gaaactgcgt ggcgaaccgt ctgaagggat gctgtgctcc
     5761 ttctctgagc tgggaatttc tgacgatcat aacggcatta tcgagctacc tgcggatgcg
     5821 ccgattggca ccgacatccg cgaatacctg aaactcgatg acaacaccat cgaaatcagc
     5881 gtgacaccaa accgtgccga ctgcttaggt atcattggtg ttgcgcgcga cgttgccgtg
     5941 ttgaaccagc tgccgctggt tgaaccggaa atcgttccgg ttggtgcgac catcgacgac
     6001 acgctgccga ttgcagtaga agcgccggat gcctgcccgc gctatcttgg ccgtgttgta
     6061 aaaggcatta acgttaaagc gccaactcct ctgtggatga aagaaaaact gcgtcgttgc
     6121 gggatccgtt ctatcgatgc agttgttgac gtcaccaact atgtgctgct cgaactgggc
     6181 cagccgatgc acgctttcga taaagatcgc attgaaggcg gcattgtggt gcggatggca
     6241 aaagagggtg aaacgctggt attgctcgat ggtactgaag cgaagctgaa tgctgacacc
     6301 ctggtcatcg ccgaccacaa caaggcgctg gcgatgggcg gcatctttgg tggcgaacac
     6361 tctggcgtga atgacgaaac gcaaaacgtt ctgctggaat gcgctttctt cagcccgctg
     6421 tctatcaccg gtcgtgctcg tcgtcatggc ctgcatactg atgcgtctca ccgttatgag
     6481 cgtggcgttg atccggcact gcagcacaaa gcgatggaac gtgcgacccg tctgctgatc
     6541 gacatctgcg gtggtgaagc gggtccggta attgatatca ctaacgaagc aacgctgccg
     6601 aagcgtgcaa ccatcacttt acgtcgtagc aaactggatc gcctgatcgg ccatcatatt
     6661 gctgatgagc aggtaacaga cattctgcgt cgtctcggct gcgaagtgac cgaaggcaaa
     6721 gacgagtggc aggcagttgc gccgagctgg cgtttcgaca tggagattga agaagatctg
     6781 gtcgaagaag tcgcgcgtgt ttacggctac aacaacatcc cggatgagcc ggtacaggca
     6841 agcctgatta tggggactca ccgtgaagct gacctgtcgc tcaagcgcgt aaaaacgctg
     6901 ctcaacgaca aaggctatca ggaagtgatc acctacagct tcgttgatcc gaaagtgcag
     6961 cagatgatcc atccaggcgt tgaagcctta ctgctgccaa gcccgatctc tgttgaaatg
     7021 tcagcaatgc gtctttctct gtggaccggc ctgctggcaa ccgtggtgta caaccagaac
     7081 cgtcagcaga atcgtgtacg cattttcgaa agcggtctgc gtttcgtgcc agatactcag
     7141 gcaccgttgg gtattcgtca ggatctgatg ttagccggtg tgatttgcgg taaccgttac
     7201 gaagagcact ggaacctggc aaaagagacc gttgatttct atgatttgaa aggcgatctt
     7261 gaatccgttc tcgacctgac cggtaaactg aatgaggttg agttccgtgc agaagcgaat
     7321 ccggcactgc atccggggca atccgcagcg atttatctga aaggtgaacg tattggtttt
     7381 gttggggttg ttcatcctga actggaacgt aaactggatc ttaacggtcg cactctggtg
     7441 ttcgaactgg agtggaacaa gctcgcagac cgcgtggtgc ctcaggcgcg cgagatttct
     7501 cgcttcccgg cgaaccgtcg tgacatcgcg gtggtggtcg cagaaaacgt tcccgcagcg
     7561 gatattttat ccgaatgtaa gaaagttggc gtaaatcagg tagttggcgt aaacttattt
     7621 gacgtgtacc gcggtaaggg tgttgcggag gggtataaga gcctcgccat aagcctgatc
     7681 ctgcaagata ccagccgtac actcgaagaa gaggagattg ccgctaccgt cgccaaatgt
     7741 gtagaggcat taaaagagcg attccaggca tcattgaggg attgaaccta tggcgcttac
     7801 aaaagctgaa atgtcagaat atctgtttga taagcttggg cttagcaagc gggatgccaa
     7861 agaactggtt gaactgtttt tcgaagagat ccgtcgcgct ctggaaaacg gcgaacaggt
     7921 gaaactctct ggttttggta acttcgatct gcgtgataag aatcaacgcc cgggacgtaa
     7981 cccgaaaacg ggcgaggata ttcccattac agcacggcgc gtggtgacct tcagacccgg
     8041 gcagaagtta aaaagccggg tcgaaaacgc ttcgcccaaa gacgagtaat ctgatctaac
     8101 taaaaaggcc gctctgcggc cttttttctt ttcactgtcg aagagtcacc gtaaaatcaa
     8161 cgccatgaca cttcagcaga acggatacca tgctgacact tgcccgccaa caacagcgac
     8221 aaaatattcg ctggttatta tgcctgtcag ttttgatgct gctggcgctt ctcttaagcc
     8281 tttgcgccgg tgaacaatgg attttgccag gtgactggtt ttctcctcgt ggcgaactgt
     8341 ttgtctggca aattcgcctg ccacgtacgc tggctgtatt gctggttggt gcggcgctgg
     8401 ctatatccgg cgctgtaatg caggcgttgt ttgaaaatcc tctggcagaa cctggactac
     8461 ttggcgtctc aaatggcgca ggcgtggggc ttatcgccgc ggtattgctt gggcaagggc
     8521 aactccccaa ctgggcgcta gggctgtgtg cgattgcggg cgcacttatc atcactttaa
     8581 tactcttacg tttcgcccgt cgtcatcttt cgaccagtcg gttattgctg gctggcgttg
     8641 cattagggat tatctgtagc gcactaatga cgtgggctat ctacttttcc acctctgttg
     8701 atttacgtca gctgatgtac tggatgatgg gcggttttgg cggcgtagac tggcggcaaa
     8761 gctggctgat gctggcattg atccccgtgt tgttgtggat ctgttgtcag tccaggccga
     8821 tgaatatatt agcacttggc gagatctcgg cgcggcaact gggtttaccc ctgtggttct
     8881 ggcgcaatgt gctggtggca gcgaccggct ggatggttgg cgtcagtgtg gcgctggcgg
     8941 gtgctatcgg ctttattggt ctggtgatcc cacatattct ccggctgtgt ggtttaaccg
     9001 atcatcgcgt attacttccc ggctgcgcgc tggcaggggc gagcgcattg ctgctggccg
     9061 atattgtagc gcgcctggca ttagctgccg cagagctgcc aattggcgtg gtcaccgcaa
     9121 cgttgggtgc gccggtgttt atctggttat tgttaaaagc aggacgttag ccgcaaaaga
     9181 cggtctatga ttaaaagcta agatttttac tgaccacacc caggagaaac gatgcaagat
     9241 tccattctga tgaccgtagt gaaagatatc gacggtgaag tgaccacgct ggagaagtac
     9301 gccggtaatg tgctgttgat tgtcaatgtc gcctcaaagt gtggcttaac gccgcaatat
     9361 gagcagttgg agaatattca gaaagcctgg gctgatcgtg gttttgtggt gctgggattc
     9421 ccgtgcaacc agtttctgga gcaagaaccg ggtagcgatg aagagattaa aacttactgc
     9481 accaccacat ggggggtgac gttcccgatg ttcagtaaga ttgaagttaa tggcgaagga
     9541 cgccatccgc tgtatcaaaa attgattgcc gcagcgccga ctgcagtcgc gccggaagag
     9601 agcggattct atgcccgtat ggtcagcaaa ggtcgtgcac cgctgtaccc tgatgatatt
     9661 ttatggaatt ttgagaaatt tttagttggc agggacggat tggtcatcca gcgtttttcc
     9721 ccggatatga cgccggaaga tcccattgtg atggaaagca ttaaactggc gttggcaaaa
     9781 taatgtctat tgtgatgcag ttacaggatg ttgcggaatc tacccgcctg gggccgcttt
     9841 ctggcgaggt tcgggctggg gagatcctgc acctggtggg gccgaatggc gcgggtaaga
     9901 gtaccttact ggcgcgaatg gccggaatga ccagcggtaa gggaagcatt cagttcgcgg
     9961 ggcaaccact ggaagcatgg tctgcaacaa agctcgcgct gcatcgcgcc tatctttcac
    10021 aacagcagac gccgccgttt gcaatgccgg tctggcacta cctgacactg catcagcacg
    10081 ataaaacgcg taccgaacta ctgaatgatg tcgcaggggc gctggctctt gatgacaaac
    10141 tcggacgtag caccaatcaa ctttccggcg gtgaatggca acgcgtacgt cttgctgcgg
    10201 tggtgctgca aatcacacca caagccaatc ccgcaggcca attgctgctt cttgatgagc
    10261 cgatgaacag tcttgatgtt gcgcaacaaa gtgccttaga caaaattctg agcgcgctgt
    10321 gtcagcaagg actggcgatt gtgatgagca gtcacgatct caaccacaca ttgcgtcatg
    10381 cgcatcgggc gtggttgcta aaaggtggaa aaatgctggc cagtggacgc agagaagagg
    10441 tgctcacgcc ggcgaatctg gcgcaggcct atgggatgaa ttttcgccgt ctggatatcg
    10501 aaggtcacag aatgctgatt tcgaccatct gatttcatta gtatcttgaa aatcgggtaa
    10561 ttacgacgct aaattaatgc aagaataaaa aacagaggaa ccgtacggaa tgcgtttctg
    10621 ccttattttg atcacagcac tgtttctggc cgggtgtagc caccataaag caccgccgcc
    10681 aaatgccaga ctttctgatt cgattaccgt tattgccggt ttgaacgacc agctacaaag
    10741 ctggcatggc acgccgtatc gttatggtgg catgacgcgg cgcggtgtgg actgttcggg
    10801 atttgtggtt gtgacgatgc gcgatcgttt cgatttgcag ctgccccgtg aaaccaaaca
    10861 acaagcctct attggcacgc aaattgataa agacgagttg ctgccaggtg acctggtctt
    10921 tttcaaaacg ggttccggac aaaatggttt gcatgtaggt atttacgata ccaacaacca
    10981 atttatccac gcctctacca gcaagggagt gatgcgttcc tcacttgata atgtctattg
    11041 gcagaaaaat ttctggcagg cgagacgaat ctagtatgtc gaaaaatgga cggcgatgat
    11101 acatcgccgt ccatttactc ctgaatacag tagtattttg cagacaaaaa ttccgtaaat
    11161 ggattcttcg tttataatca ggataatcta aaaaaaggac ccctgaaaaa gtggttaaca
    11221 tggaacaaat gaaagttact ccttctgtaa ttgttatgtc ttatggcctc atccctttta
    11281 gccggatact gaaaaacatc cttcgagagg gacggttacc atgaagattt ttttggagaa
    11341 tctttatcat tctgattgct atttcctgcc gataagagat aaccagcaag accttgtcgg
    11401 cgttgagtta attacccatt tctccagtga agatggtacg gttcgtatcc cgacgagtcg
    11461 ggttatagcg caacttaccg aggagcaaca ttggcaatta ttttctgaac agttggaatt
    11521 actaaaatca tgccaacact tttttattca acataaactc tttgcctggc ttaatttaac
    11581 gccacaagtg gctacattat tattagacag ggataatttc gcaggcgaac tattaaaata
    11641 tccgtttatt gaactgttga ttaatgagaa ttacccgcat ttcaatgaag gaaaagataa
    11701 tcgcgatctt ttatccttat cgcaaatgta tccccttgtg ttaggtaatt tgggagcagg
    11761 taatagcaca atgaaagcgg tttttgatgg tcttttcacc cgcgtcatgc tggataaaag
    11821 ttttattcag caacaaatca cgcatcgttc cttcgaaccg tttattcgtg cgatccaggc
    11881 gcaaatttcc ccttgttgca attgcatcat cgcggggggc attgatacgc cagaaatatt
    11941 ggcacagatt atcccctttg atttccatgc tctccaggga tgcctgtggc ctgcggttcc
    12001 tataaatcag ataacgacgt tggttcagcg ataaccctcc tgtttgctgg tgtttaggac
    12061 gagagtaacc gtctacacta tcaaacagga ggatctatga ccctgtcttt tattacccgc
    12121 tggtgcgatg aattgccaga aacctataca gcactttccc ctacgccttt aaataatgcc
    12181 cggctgattt ggcataatac cgaactcgct aacacgctga gtattccatc gtcgctgttt
    12241 aaaaatggcg caggtgtctg gggcggcgaa accctactgc ctggtatgtc accactggcg
    12301 caggtttaca gtggtcatca gttcggcgtc tgggcgggcc aactgggtga tgggcgcggc
    12361 attttactcg gcgaacaact gcttgctgat ggcactacaa tggactggca tctgaaaggt
    12421 gctggcctga cgccttattc gcgaatgggt gatggacggg cggttttacg ttcgacgata
    12481 cgagaaagtc tcgccagtga ggcgatgcat tatctgggca ttccgacgac ccgcgcgtta
    12541 agtatcgtca ccagcgattc gccagtgtat cgggaaacgg tggagccagg cgcgatgctg
    12601 atgcgtgtgg caccaagtca tctgcgcttt ggtcatttcg aacattttta ctatcgccgc
    12661 gagccggaaa aggttcgtca gttagctgac tttgccattc gtcattactg gtcacatctt
    12721 gatgatgagg aggacaaata ccgtctctgg tttaccgatg ttgtcgcacg taccgcctcg
    12781 ttaattgccc aatggcagac ggtcggcttt gctcatgggg tgatgaatac cgacaacatg
    12841 tcgctgctgg ggctgacgct tgattacggg ccgtttggtt ttcttgatga ttacgaaccc
    12901 ggttttattt gtaatcactc ggatcatcaa gggcgttaca gctttgataa tcaacctgcc
    12961 gtcgcgttgt ggaatttaca gcgtctggcg cagacattgt cgccatttgt tgccgtagat
    13021 ggcctgaatg aggccctgga cagctatcag caggttttgt tgacgcatta tggacaacgg
    13081 atgcggcaga aactgggctt catgacggag caaaaagagg ataacgcgct actgaatgaa
    13141 ttattcagtc tgatggcgcg agagcgcagc gattataccc gcacattccg catgctgagt
    13201 ctgaccgagc agcacagcgc ggcgtcactg ctacgtgatg agtttatcga tcgtgcggca
    13261 tttgatgact ggtttgcccg ttatcgggta cgtttgcaac aagacgaggt taccgatagt
    13321 gagcgtcagc agctgatgca aagcgttaac ccagctctgg tgttgcgcaa ctggttggcg
    13381 caacgggcga ttgaagcgtc agaaaagggt gatatgacgg aattgcaccg cctgcatgag
    13441 gcgttgcgaa atcctttcag cgacagagat gatgactacg tcagtcgtcc acctgactgg
    13501 ggtaaacggc tggaagtcag ctgctcgagt taaaggttac ttggtcaaca gcagcttgcc
    13561 agcctgagtt ttacggagca gatattcttg cccgtcatga tcgataatca gtttgccatc
    13621 cggacctaac agtgtctggc tggtaattcg ccggatgggc tgcggggaag cggtcttgtg
    13681 actggcatcc gtttcaggcg tgagttttct gctatccgta taacgcataa aaatt
//
LOCUS       DADSYM010000131        13715 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2415
            SAMN08039579-rid11723173.denovo.132, whole genome shotgun sequence.
ACCESSION   DADSYM010000131 DADSYM010000000
VERSION     DADSYM010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039579
            Sequence Read Archive: SRR6362533
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13715)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13715)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980162.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 03:39:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,484
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 4,347
            CDSs (with protein)               :: 4,347
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 43 of 85
            Pseudo Genes (incomplete)         :: 37 of 85
            Pseudo Genes (internal stop)      :: 19 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13715
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039579-rid11723173.denovo.132"
                     /strain="LGE2415"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8302 N 95.9628 W"
                     /collection_date="2015-08-24"
     gene            19..1473
                     /locus_tag="J1Y83_003416"
     CDS             19..1473
                     /locus_tag="J1Y83_003416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative allantoin permease"
                     /protein_id="HBA7631532.1"
                     /translation="MEHQRKLFQQRGYSEDLLPKTQSQRTWKTFNYFTLWMGSVHNVP
                     NYVMVGGFFILGLSTFSIMLAIILSAFFIAAVMVLNGAAGSKYGVPFAMILRASYGVR
                     GALFPGLLRGGIAAIMWFGLQCYAGSLACLILIGKIWPGFLTLGGDFTLLGLSLPGLI
                     TFLLFWLVNVGIGFGGGKVLNKFTAILNPCIYIVFGGMAIWAISLVGIGPIFDYIPGG
                     IQKAENSGFLFLVVINAVVAVWAAPAVSASDFTQNAHSFREQALGQTLGLVVAYILFA
                     VAGVCIIAGASIHYGADTWNVLDIVQRWDSLFASFFAVLVILMTTISTNATGNIIPAG
                     YQIAAIAPTKLTYKNGVLIASIISLLICPWKLMENQDSIYLFLDIIGGMLGPVIGVMM
                     AHYFVVMRGQINLDELYTAPGDYKYYDNGFNLTAFSVTLVAVILSLGGKFIPFMEPLS
                     RVSWFVGVIVAFAAYALLKKRTTAEKTGEQKTIG"
     gene            1533..2894
                     /gene="allB"
                     /locus_tag="J1Y83_003417"
     CDS             1533..2894
                     /gene="allB"
                     /locus_tag="J1Y83_003417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="allantoinase AllB"
                     /protein_id="HBA7631533.1"
                     /translation="MSFDLIIKNGTVILENEARVVDIAVKDGKIAAIGQDLGDAKDVM
                     DASGLVVSPGMVDAHTHISEPGRSHWEGYETGTRAAAKGGITTMIEMPLNQLPATVDR
                     ASIELKFDAAKGKLTIDAAQLGGLVSYNIDRLHELDEVGVVGFKCFVATCGDRGIDND
                     FRDVNDWQFFKGAQKLGELGQPVLVHCENALICDALGEEAKREGRVTAHDYVASRPVF
                     TEVEAIRRVLYLAKVAGCRLHVCHVSSPEGVEEVTRARQEGQDVTCESCPHYFVLDTD
                     QFEEIGTLAKCSPPIRDLENQKGMWEKLFNGEIDCLVSDHSPCPPEMKAGNIMKAWGG
                     IAGLQSCMDVMFDEAVQKRGMSLPMFGKLMATNAADIFGLQQKGRIAPGKDADFVFIQ
                     PNSSYVLTNDDLEYRHKVSPYVGRTIGARITKTILRGDVIYDIEQGFPVAPKGQFILK
                     HQQ"
     gene            2950..4251
                     /locus_tag="J1Y83_003418"
     CDS             2950..4251
                     /locus_tag="J1Y83_003418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308602.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil/xanthine transporter"
                     /protein_id="HBA7631534.1"
                     /translation="MFNFAVGRESLLSGFQWFFFIFCNTVVVPPTLLSAFQLPQSSLL
                     TLTQYAFLATALACFAQAFCGHRRAIMEGPGGLWWGTILTITLGEASRGTPINDIATS
                     LAVGIALSGVLTMLIGFSGLGHRLARLFTPSVMVLFMLMLGAQLTTIFFKGMLGLPFG
                     IADPNFKIQLPPFALSVAVMCLVLAMIIFLPQRFARYGLLVGTITGWLLWYFCFPSSH
                     SLSGELHWQWFPLGSGGALSPGIILTAVITGLVNISNTYGAIRGTDVFYPQQGAGNTR
                     YRRSFVATGFMTLITVPLAVIPFSPFVSSIGLLTQTGDYTRRSFIYGSVICLLVALVP
                     ALTRLFCSIPLPVSSAVMLVSYLPLLFSALVFSQQITFTARNIYRLALPLFVGIFLMA
                     LPPVYLQDLPLTLRPLLSNGLLVGILLAVLMDNLIPWERIE"
     gene            4273..5418
                     /gene="glxK"
                     /locus_tag="J1Y83_003419"
     CDS             4273..5418
                     /gene="glxK"
                     /locus_tag="J1Y83_003419"
                     /EC_number="2.7.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate 3-kinase"
                     /protein_id="HBA7631535.1"
                     /translation="MKIVIAPDSFKESLSAEKCCQAIKAGFSTIFPDAHYICLPIADG
                     GEGTVEAMVAATDGNIVKLEVCGPMGEKVNAFYGITGDGKTAVIEMAAASGLMLVAPE
                     KRNPLLASSFGTGELIRHALDNGIRHIILGIGGSATVDGGMGMAQALGVRFLDADGQP
                     LAANGGNLARVASIEMNECDPRLANCHIEVACDVDNPLVGARGAAVVFGPQKGATPEM
                     VEELEQGLQNYARVLQQLTEINVCQMAGGGAAGGMGIAAAVFLNADIKPGIEIVLNAV
                     NLAQAVQGAALVITGEGRIDSQTAGGKAPLGVASVAKQFNVPVIGIAGVLGDGVEVVH
                     QYGIDAVFSILPRLAPLAEVLASGETNLFNSARNIACAIKIGQGIKN"
     gene            complement(5547..6332)
                     /gene="allE"
                     /locus_tag="J1Y83_003420"
     CDS             complement(5547..6332)
                     /gene="allE"
                     /locus_tag="J1Y83_003420"
                     /EC_number="3.5.3.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(S)-ureidoglycine aminohydrolase"
                     /protein_id="HBA7631536.1"
                     /translation="MGYLNNVTGYREDLLANRAIVKHGNFALLTPDGLVKNIIPGFEN
                     CDATILSTPKLGASFVDYLVTLHQNGGNQQGFGGEGIETFLYVISGNITAKAEGKTFT
                     LSEGGYLYCPPGSLMTFVNAQAEDSQIFLYKRRYIPVEGHAPWLVSGNASELERIHYE
                     GMDDVILLDFLPKELGFDMNMHILSFAPGASHGYIETHVQEHGAYILSGQGVYNLDNN
                     WIPVKKGDYIFMGAYSLQAGYGVGRGEAFSYIYSKDCNRDVEI"
     gene            complement(6343..7578)
                     /gene="allC"
                     /locus_tag="J1Y83_003421"
     CDS             complement(6343..7578)
                     /gene="allC"
                     /locus_tag="J1Y83_003421"
                     /EC_number="3.5.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="allantoate deiminase"
                     /protein_id="HBA7631537.1"
                     /translation="MITHFRQAIEETLPWLSSFGADPAGGMTRLLYSPEWLETQQQFK
                     KRMAASGLETRFDEVGNLYGRLSGTEYPQEVVLSGSHIDTVVNGGNLDGQFGALAAWL
                     AIDWLKTQYGAPLRTVEVVAMAEEEGSRFPYVFWGSKNIFGLANPDDVRNICDAKGNS
                     FVDAMKACGFTLPNAPLTPRQDIKAFVELHIEQGCVLESNGQSIGVVNAIVGQRRYTV
                     TLNGESNHAGTTPMGYRRDTVYAFSRICHQSVEKAKKMGDPLVLTFGKVEPRPNTVNV
                     VPGKTTFTIDCRHTDATVLRDFTQQLENDMRAICDEMDIGIDIDLWMDEEPVPMNKEL
                     VATLTELCESEKLNYRVMHSGAGHDAQIFAPRVPTCMIFIPSINGISHNPAERTNITD
                     LAEGVKTLALMLYQLAWQK"
     gene            complement(7600..8649)
                     /gene="allD"
                     /locus_tag="J1Y83_003422"
     CDS             complement(7600..8649)
                     /gene="allD"
                     /locus_tag="J1Y83_003422"
                     /EC_number="1.1.1.154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ureidoglycolate dehydrogenase"
                     /protein_id="HBA7631538.1"
                     /translation="MKISRETLHQLIENKLCQAGLKREHAATVAEVLVYADARGIHSH
                     GAVRVEYYAERISKGGTNREPEFRLEETGPCSAILHADNAAGQVAAKMGMEHAIKTAQ
                     QKGVAVVGISRMGHSGAISYFVQQAARAGLIGISLCQSDPMVVPFGGAEIYYGTNPLA
                     FAAPGEGDEILTFDMATTVQAWGKVLDARSRNMSIPDTWAVDKNGAPTTDPFAVHALL
                     PAAGPKGYGLMMMIDVLSGVLLGLPFGRQVSSMYDDLHAGRNLGQLHIVINPNFFSSS
                     ELFRQHLSQTMRELNTITPAPGFNQVYYPGQDQDIKQRKAAVEGIEIVDDIYQYLISD
                     ALYNTSYETKNPFAQ"
     gene            8966..10633
                     /locus_tag="J1Y83_003423"
     CDS             8966..10633
                     /locus_tag="J1Y83_003423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase FdrA"
                     /protein_id="HBA7631539.1"
                     /translation="MIHAFIKKGCFQDSVSLMIISRKLSESENVDDVSVMMGTPANKA
                     LLDTTGFWHDDFNNATPNDICVAIRSEAADAGIAQAVMQQLEEALKQLAQGSGSSQAL
                     TQVRRWDSASQKLPDANLALISVAGEYAAELANQALDRNLNVMMFSDNVTLEDEIQLK
                     TRAREKGLLVMGPDCGTSMIAATPLAFANVMPEGNIGVIGASGTGIQELCSQIALAGE
                     GITHAIGLGGRDLSREVGGISALTALEMLSADEKSEVLAFVSKPPAETVRLKIVNAMK
                     ATGKPTVALFLGYTPAVARDENVWFASSLDEAARLACLLSRVTARRNAIAPVSSGFIC
                     GLYTGGTLAAEAAGLLAGHLGVEADDTHQHGMMLDADGHQILDLGDDFYTVGRPHPMI
                     DPTLRNLLIADLGAKPQVRVLLLDVVIGFGATADPAASLVSAWQKACAARSDNQPLYA
                     IATVTGTERDPQCRSQQIATLEDAGIAVVSSLPEATLLAAALIHPLSSATQQHTPSLL
                     ENVAVINIGLRSFALELQSASKPVVHYQWSPVAGGNKKLARLLERLQ"
     gene            10643..11902
                     /locus_tag="J1Y83_003424"
     CDS             10643..11902
                     /locus_tag="J1Y83_003424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009029994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1116 domain-containing protein"
                     /protein_id="HBA7631540.1"
                     /translation="MFTSVAQANAAVIEQIRRARPHWLDVQPASSLISELNKGKTLLH
                     AGPPMRWQEMTGPMKGACVGACLFEGWAKDEAQALAILEQGEVNFIPCHHVNAVGPMG
                     GITSASMPMLVVENVTDGNLAYCNLNEGIGKVMRFGAYGEDVLTRHRWMRDVLMPVLS
                     AALGRMERGIDLTAMMAQGITMGDEFHQRNIASSALLMRALAPQIARLDHDKQHIAEV
                     MDFLSVTDQFFLNLAMAYCKAAMDAGAMIRAGSIVTAMTRNGNMFGIRVSGLGERWFT
                     APVNTPQGLFFTGFSQEQANPDMGDSAITETFGIGGAAMIAAPGVTRFVGAGGMEAAR
                     AVSEEMAEIYLERNMQLQIPGWDFQGACLGLDIRRVVETGITPLINTGIAHKEAGIGQ
                     IGAGTVRAPLACFEQALEALAESMGIG"
     gene            11913..12728
                     /locus_tag="J1Y83_003425"
     CDS             11913..12728
                     /locus_tag="J1Y83_003425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2877 domain-containing protein"
                     /protein_id="HBA7631541.1"
                     /translation="MTIIHPLLASRSAPNYRQSWRLAGVWRRAINLMTESGELLTLHR
                     QGSGFGPGGWVLRRAQFDALCGGLCGNERPQVVAQGIRLGRFTVKQPQRYCLLRITPP
                     AHPQALAVAWMQRAEETGLFGPLALAASDPLPAELRQFRHCFQAALNGVKTDWRHWLG
                     KGPGLTPSHDDTLSGMLLAAWYYGALDARSGRPFFACSDNLQLVTTAVSVSYLRYAAQ
                     GYFASPLLHFVHALSCPKRTAAAIDSLLALGHTSGADTLLGFWLGQQLLQGKP"
     gene            12725..13618
                     /locus_tag="J1Y83_003426"
     CDS             12725..13618
                     /locus_tag="J1Y83_003426"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HBA7631542.1"
                     /translation="MKTLVVALGGNALLQRGEALTAENQYRNIASAVPALARLARSYR
                     LAIVHGNGPQVGLLALQNLAWKEVEPYPLDVLVAESQGMIGYMLAQSLSAQPQMPPVT
                     TVLTRIEVSPDDPAFLQPEKFIGPVYQPEEQEALEAAYGWQMKRDGKYLRRVVASPQP
                     RKILDSEAIELLLKEGHVVICSGGGGVPVTEDGAGSEAVIDKDLAAALLAEQINADGL
                     VILTDADAVYENWGTPQQRAIRHATPDELAPFAKADGSMGPKVTAVSGYVRSRGKPAW
                     IGALSRIEETLAGEAGTCISL"
BASE COUNT         3070 a         3343 c         3833 g         3469 t
ORIGIN      
        1 tgaaattaag agggatttat ggaacatcag agaaaactat tccagcaacg cggctatagc
       61 gaagatctac tgccgaaaac gcaaagtcag cggacctgga aaacatttaa ctattttacc
      121 ttatggatgg gttcggttca taacgttccc aattatgtga tggttggcgg cttttttatt
      181 ctcggcttgt ctacctttag tattatgctg gcaattatcc tcagtgcctt tttcattgcc
      241 gcggtaatgg tattaaacgg tgctgcgggc agtaaatacg gcgtaccgtt tgccatgatc
      301 ctgcgtgctt cttacggcgt acgtggtgca ctgtttcccg gattattaag aggcggtatt
      361 gctgccatta tgtggtttgg cctgcaatgt tacgcggggt cactggcctg cttgattctg
      421 attggcaaaa tctggccggg atttttgact ctcggtggcg atttcacgct gttaggcctt
      481 tctctaccgg gattaattac ttttttactc ttctggctgg ttaacgttgg tattggtttt
      541 ggcggcggaa aagttttaaa taaattcact gccattctta acccgtgcat ctatatcgtt
      601 ttcggcggta tggcgatttg ggcgatttcg ctggtcggga tcggtccaat ctttgactac
      661 attccgggcg gtattcagaa agcagaaaac agtggcttcc tgtttctggt ggtgataaac
      721 gcggtggttg cggtctgggc ggcaccggcg gtgagcgcat ccgactttac gcaaaacgcc
      781 cactcgtttc gtgagcaggc gctggggcaa acgctgggtt tagttgtggc ctatattctg
      841 tttgcggtcg ccggggtatg tattattgcc ggagccagta ttcattacgg cgctgacacc
      901 tggaacgtgc tggatattgt tcagcgttgg gacagcctgt tcgcctcgtt ctttgcggta
      961 ctggttattc tgatgacgac tatctctact aacgccaccg gtaatattat tccggctggt
     1021 tatcagattg ccgccattgc accgacgaaa ctgacctata aaaacggcgt actgattgcc
     1081 agtattatca gcctgctgat ctgcccgtgg aaattaatgg aaaatcagga cagtatttat
     1141 ctgttcctcg atattatcgg cggaatgctt ggtccggtaa ttggtgtgat gatggcgcat
     1201 tattttgtgg tgatgcgtgg gcaaattaat cttgatgaac tgtacaccgc acctggcgat
     1261 tataaatatt acgataacgg ttttaacctc accgcgtttt cagtaactct ggtggccgtt
     1321 attttatctc ttggcggtaa gtttattccc tttatggaac cgttatcacg tgtttcatgg
     1381 tttgtcggcg tcatcgtcgc ctttgcggcc tacgccttat taaagaaacg tacaacagca
     1441 gaaaaaacag gagaacaaaa aaccataggt taattaatca cgatattgaa cattgagtta
     1501 aaaaccaatc tgtattttac aaggagtttg ttatgtcttt tgatttaatc attaaaaacg
     1561 gcaccgttat tttagaaaac gaagctcgcg ttgtagatat cgccgttaaa gacggaaaaa
     1621 ttgctgctat cggccaggat ctgggcgatg caaaagacgt tatggatgcg tctggtctgg
     1681 tggtttcgcc gggcatggtt gatgcgcaca cccatatttc tgaaccgggt cgcagccact
     1741 gggaaggtta tgaaaccggt actcgcgcag cagcaaaagg tggtatcacc accatgatcg
     1801 aaatgccgct caaccagctg cctgcaacgg ttgaccgcgc ctcaattgaa ctgaagttcg
     1861 atgccgctaa aggaaagctg actatcgatg cggcacaact cggtggcctg gtgtcttaca
     1921 acattgaccg tctgcatgag ttggatgaag tgggtgttgt cggtttcaaa tgcttcgttg
     1981 cgacctgtgg cgatcgcggt atcgacaacg acttccgtga cgtcaatgac tggcaattct
     2041 tcaaaggcgc gcagaagctg ggtgaactag gccagccggt gctggtgcac tgcgaaaacg
     2101 cgctgatctg tgacgcactg ggcgaagaag cgaagcgtga aggtcgcgta actgcccatg
     2161 actatgtggc ttcgcgtccg gtatttaccg aagtggaagc gattcgccgc gtactgtacc
     2221 tggcgaaagt cgctggttgc cgtctgcacg tttgccacgt cagcagcccg gaaggtgttg
     2281 aggaagtgac tcgtgcacgt caggaaggtc aggatgttac ttgtgaatcc tgcccgcatt
     2341 actttgtgct ggataccgat cagttcgaag aaatcggtac tctggcgaag tgttcaccgc
     2401 cgatccgcga tctggaaaac cagaaaggca tgtgggaaaa actgtttaac ggtgaaatag
     2461 actgcctggt ttccgaccac tctccatgcc cgccggaaat gaaagccggt aacatcatga
     2521 aagcgtgggg cggtatcgct ggtctgcaaa gctgcatgga cgtgatgttc gatgaagcgg
     2581 tacagaaacg cggaatgtct ctgccaatgt tcggcaaatt aatggcgact aacgcagcag
     2641 atattttcgg tctgcagcaa aaaggccgta tcgccccagg aaaagatgcc gacttcgtct
     2701 tcattcagcc gaatagcagc tatgttctta ccaatgacga tctggaatat cgccacaaag
     2761 tcagcccgta tgttggccgt actattggcg cgcgtatcac gaaaaccatc ttacgtggtg
     2821 atgtgattta cgatatcgaa cagggcttcc ctgttgcgcc gaaaggtcaa tttatcctta
     2881 aacatcagca gtaatctggc cctgcaatgc ccgtccttgt ggcgggcatt ctccggttaa
     2941 ggtgtgttta tgttcaattt tgcagtgggc cgcgaaagcc tgttatcagg atttcagtgg
     3001 tttttcttta ttttttgcaa cacggttgtg gttcctccta cgctactttc tgcttttcag
     3061 ttgccgcaaa gtagcctgct tacgctcacg caatatgctt ttcttgctac cgcactggcc
     3121 tgcttcgctc aggcgttctg cggtcatcgt cgcgctatta tggaggggcc gggcggcctg
     3181 tggtggggaa ccatcctgac tatcaccctt ggcgaagcat cgcgcgggac accgatcaac
     3241 gatatcgcta ccagtctggc ggtggggatt gcgctctccg gcgtgctgac gatgttgatt
     3301 ggttttagcg gattaggcca tcgcctggca cggttattta cgccgtcggt gatggtcttg
     3361 tttatgttga tgctgggtgc gcaactgacc actatctttt tcaaaggtat gctcgggctg
     3421 ccgtttggca tagccgaccc gaattttaaa attcaattac cgccgttcgc gctctcggtg
     3481 gcggtgatgt gcctggtact ggcgatgatt attttcctgc cacaacgttt tgcccgttac
     3541 ggcctgctgg tcggtacgat aaccggctgg ttgttgtggt acttttgctt tccttcttcg
     3601 cattcgctct ccggtgagtt gcactggcag tggttcccgc tcggcagtgg cggtgctttg
     3661 tcgccgggga ttattctgac ggcggtgatt acaggtctgg taaatatcag caatacctac
     3721 ggtgcgattc ggggcacgga tgttttttat ccgcagcagg gcgcggggaa tacgcgttat
     3781 cgtcgcagct ttgtggcgac cggatttatg acactgataa ccgtgccgct ggcggtaatt
     3841 ccattttcac cgtttgtttc atccattggt ttattaaccc agactggcga ttacacgcgg
     3901 cgttcgttta tttatggcag cgttatttgc ctgctggtgg cgctggttcc ggcactcacg
     3961 cgactgtttt gcagtatccc tttacccgtg agtagtgcgg tcatgctggt ttcttatctg
     4021 cctttactct tttccgcgct ggtgtttagc cagcaaataa cgtttaccgc tcgcaatatt
     4081 tatcgactcg cattgccgtt atttgtcggc atatttttaa tggcattacc gcctgtgtat
     4141 ctgcaagacc ttccattaac gcttcgtcct ctgctcagta acggcttact ggtcggaatt
     4201 ttactggctg ttcttatgga taatcttatt ccgtgggaac gcatcgaata atttgttgaa
     4261 aaaggattga taatgaagat tgtcattgcg ccagactctt ttaaagagag cttaagtgca
     4321 gaaaaatgtt gtcaggcaat taaagccggg ttttcgacca tatttcccga tgcgcactat
     4381 atctgtttgc cgatagcgga tggcggcgaa gggacggtgg aggcgatggt cgccgcgacg
     4441 gacggtaaca tcgtgaagct tgaagtctgc gggccgatgg gcgaaaaagt gaatgctttt
     4501 tatggcatta ccggcgacgg gaaaacggcg gtgattgaga tggcagcagc aagtggcctg
     4561 atgctggtcg cgcctgaaaa gcgtaatccg ttgctggcct ccagttttgg tacgggggag
     4621 ttaattcgcc atgcgctgga taatggcatt cgccatatta ttctcggcat tggcggcagt
     4681 gcgacggtcg acggcggtat gggcatggcg caggcgctcg gtgtgcgttt ccttgatgcc
     4741 gacggtcagc cgctggcggc taacggcggc aatttagcgc gcgtggcaag cattgagatg
     4801 aatgaatgcg atccgcgtct ggcgaactgt catattgaag tagcatgtga cgttgataac
     4861 ccgctggtag gggcacgcgg cgcggcggtg gtgtttggcc cgcaaaaagg ggcaacgccg
     4921 gagatggtcg aagagctgga acaggggctg caaaattacg cccgtgtttt acaacagcta
     4981 actgaaatca acgtctgcca gatggcgggt ggtggcgcgg cgggcggtat gggcattgcg
     5041 gcggcggtat tcctcaatgc ggatattaaa ccgggtattg agattgtgtt gaatgcggtc
     5101 aatcttgcgc aggcagtgca gggtgcagca ctggtgatca ccggggaagg gcgcatcgac
     5161 tcgcaaacgg caggcggtaa agcgccgctg ggcgtggcgt cggtggcgaa gcagtttaat
     5221 gtcccggtga ttgggattgc tggcgtattg ggtgatggcg tggaagtggt gcaccagtac
     5281 ggcattgatg cggtattcag cattttgcca cgtctggctc ctttagccga agtgctcgcc
     5341 agcggtgaaa ccaatctctt caacagtgcg cgaaatattg cctgcgccat taaaataggt
     5401 cagggaatta aaaactgact attaccttta aagcggatgc gatttatatc gcatcttttt
     5461 ttacctgaat tgactacaaa aaaagccacg gaataaaccg tggcaaaatc caacatagct
     5521 aaaaataatc aggcgagtgg tatgacttaa atctctacgt cgcggttgca atctttcgag
     5581 taaatatagc tgaacgcttc accacgccct acgccataac cagcctgtaa agaataagcg
     5641 cccataaaga tgtaatcgcc ttttttcacc gggatccagt tattgtcgag gttataaacc
     5701 ccctgaccgg aaagaatata ggcaccgtgt tcctgaacgt gggtttcgat ataaccgtgg
     5761 ctggcacctg gtgcaaaaga gaggatatgc atgttcatat caaaacctaa ctctttgggc
     5821 agaaaatcca gcagaataac atcgtccatg ccttcataat gaatgcgttc cagttcgctg
     5881 gcattgccag aaaccagcca cggcgcatga ccttctaccg gaatatagcg acgcttatat
     5941 aaaaagattt ggctgtcttc ggcctgggcg ttaacaaacg tcattaagga gcctggtggg
     6001 caataaagat agccaccttc acttaaggta aatgttttcc cttcggcttt ggcagtgata
     6061 tttccagaga tcacatacag gaacgtttca atgccttcgc caccgaagcc ctgttggttg
     6121 ccgccgtttt gatgcagtgt gaccagataa tcaacaaaag aggcacccag ctttggcgtg
     6181 gagaggattg tcgcgtcaca attttcaaag cccggaataa tattttttac cagaccgtct
     6241 ggtgttaaca gtgcgaaatt accgtgttta acaatcgcac ggttagccag taaatcttcg
     6301 cggtaaccgg tgacgttatt taaatatccc atttataact ccttatttct gccaggcaag
     6361 ttgataaagc atgagtgcca acgttttgac cccttcggca aggtcggtaa tattggtgcg
     6421 ttccgccggg ttatggctga tcccgttgat gctgggaatg aaaatcatgc aggtcggcac
     6481 gcgaggcgcg aaaatttgcg cgtcgtgccc ggcaccactg tgcatcaccc ggtaattcag
     6541 tttttcgctt tcacacaatt ctgtcagggt ggcgaccagc tccttattca tcggcacggg
     6601 ttcttcgtcc atccataaat cgatatcaat accaatatcc atttcatcgc aaatcgcccg
     6661 catgtcgttt tctaactgtt gggtgaaatc gcgcagcacg gtagcatcgg tatgacgaca
     6721 atcaatggtg aacgtggttt tacccggcac tacattcacc gtattcgggc gcggctctac
     6781 tttgccaaag gtcagaacca gcgggtcgcc catctttttc gctttttcga cagactgatg
     6841 gcaaatgcga ctgaaagcgt aaaccgtatc acgacgataa cccatcggcg tggtgcctgc
     6901 atggtttgat tcgccattca gcgtcaccgt ataacgacgc tgcccgacaa ttgcattcac
     6961 cacgccaatt gattgcccat tactttccag cacacagccc tgttcgatat gcagttcgac
     7021 aaaggcttta atatcctgac gcggagttag tggggcgttc ggcagagtaa atccgcaagc
     7081 cttcatcgca tcgacaaaac tatttccttt ggcatcacag atattccgca cgtcgtcagg
     7141 attcgccagc ccaaaaatat ttttactgcc ccagaaaaca tacgggaagc ggctgccttc
     7201 ttcttctgcc atcgccacca cttcgaccgt acgtaacggc gcgccgtatt gcgttttcag
     7261 ccagtcaata gccagccacg ccgccagcgc accgaattgc ccgtcgaggt taccgccgtt
     7321 aaccacggta tcgatatgcg aaccgctcag aaccacttcc tgtggatatt cggtgccact
     7381 caggcgaccg tataaattcc ccacttcatc gaaacgtgtt tccagcccgc ttgctgccat
     7441 tcttttttta aactgctgct gggtttccag ccattccggc gaataaagta aacgggtcat
     7501 cccacccgct gggtcagcgc caaaagagga aagccagggc agcgtttctt ctatagcttg
     7561 acggaaatgt gtaatcataa gaaagtcctg tctcaataat tattgcgcaa agggattttt
     7621 cgtttcgtat gacgtgttat aaagcgcgtc ggaaattaaa tactggtaaa tatcatcaac
     7681 aatttcgatg ccttcgacgg cggctttgcg ttgtttaata tcctgatcct gtccgggata
     7741 ataaacctga ttaaaaccgg gcgcgggggt aatggtattt aattcgcgca tggtctggct
     7801 aagatgttga cggaataatt cgctggagga gaaaaagttc gggttaataa ctatatgtaa
     7861 ttgccccaaa ttacgcccgg cgtgtaaatc gtcatacatc gaactaacct gtcgcccgaa
     7921 cggtaagccg agcaagacgc ctgagaggac gtcaatcatc atcatcaggc catacccttt
     7981 cggcccagcg gcggggagca gagcatgtac cgcgaacgga tcggttgttg gtgcaccgtt
     8041 tttatcgact gcccaggtat ccgggataga catattgcgc gagcgggcgt cgagcacttt
     8101 tccccatgcc tgtacggtag tcgccatatc aaaggtaagg atctcgtcgc cttctcccgg
     8161 cgcggcaaag gccagtgggt tagtgccgta gtaaatttcc gcgccgccaa acggcaccac
     8221 cattggatcg gactggcaca gcgaaatgcc aattaatccg gcgcgggctg cctgctgcac
     8281 aaaataagag attgcgccgc tgtgacccat ccggctgata ccgaccaccg caacgccttt
     8341 ttgctgggcg gttttgatgg catgttccat acccattttc gctgcgacct gtccggcggc
     8401 attgtcggca tgtaaaattg ccgagcacgg tccggtttcc tcaagacgaa actccggttc
     8461 gcggttggtg ccgccttttg aaatgcgttc ggcgtagtat tcaacgcgca ccgcgccatg
     8521 agagtggatc cctctggcat cggcgtaaac caatacttca gccacggttg cagcgtgctc
     8581 acgttttaac ccagcctggc agagtttatt ctcgattagc tggtggagtg tttcccgact
     8641 gattttcatc tgtcttcctt tttaacgacg gtgtgaagca tgactgcaat taacatacag
     8701 ggaaaatatc tggattatgt gatccagaca ggcaaaaaaa tatagttaga atttatttga
     8761 taatcttcgt gcttttaacc tggtttttaa aacaatcatg cttattaaaa aataacgcgt
     8821 aatagtctgg cggttatttg aattcttttg ttaataatcc acgtgtgata ttcatcacct
     8881 tatttactcg ttgtcatcga taccgtaatc gccacattaa cactgctcgt gcaattgcca
     8941 tgggtgcaat ttttaaggag ttgttatgat ccacgccttt attaaaaaag ggtgttttca
     9001 ggattcggtc agtttaatga ttatttcacg aaaactcagc gaatcagaaa atgttgatga
     9061 tgtttccgta atgatgggta cgcccgccaa taaagcgtta ttagatacta caggtttctg
     9121 gcatgacgat tttaataacg ccacgccgaa cgatatttgc gtggcaattc gtagcgaagc
     9181 ggcggatgcg gggatcgcgc aggcggttat gcagcagctt gaagaggcgc taaaacagct
     9241 ggcgcagggt tcaggcagca gccaggcgtt gacgcaggtg cgtcgctggg acagtgccag
     9301 tcagaaatta cccgatgcca atctggcgct gatttcagtg gctggtgagt atgcggcgga
     9361 gctggcgaac caggcgctgg atcgcaacct caacgtgatg atgttttccg ataacgtcac
     9421 gctggaagat gaaatccaac ttaaaacccg cgcgcgggaa aaaggcttgc tggtgatggg
     9481 gccagactgc ggtacgtcga tgattgccgc cacgccgctg gcttttgcta acgtgatgcc
     9541 ggaaggcaac attggcgtca taggcgcttc cggtaccggg attcaggagc tgtgctcaca
     9601 gattgcactg gcaggggagg gaattactca cgcgattggt cttggcgggc gcgatctcag
     9661 ccgtgaagtg ggcggcatca gtgcgctaac ggcgctggaa atgctcagtg cagacgagaa
     9721 aagcgaagtg ctggcatttg tttcaaaacc acctgccgaa actgtgcgtc tgaaaattgt
     9781 taatgccatg aaagcaactg gcaaaccgac ggtggcgctg tttttaggtt ataccccggc
     9841 ggtggcccgc gacgagaatg tctggtttgc ctcctcgctg gatgaggccg ctcgtctggc
     9901 ttgtctgctt tcacgcgtca ctgcgcgacg taacgcgata gcgcctgtca gcagcggatt
     9961 tatttgcggt ttgtataccg gcggtacgct ggctgctgaa gcggcgggat tacttgccgg
    10021 acaccttggc gtggaagccg acgataccca tcaacatggc atgatgctgg acgccgacgg
    10081 acatcagatc ctcgacctgg gcgatgattt ttacaccgtc gggcgtcccc atccgatgat
    10141 cgacccgacc ttacgcaacc tgttaattgc cgatctcggc gctaaaccgc aagtgcgcgt
    10201 gttgctgctt gatgtcgtga ttggcttcgg tgcgaccgcc gatcctgccg cctcgctggt
    10261 gagcgcctgg caaaaagcct gtgccgcgcg ttcagacaat caaccactgt atgccattgc
    10321 cacggtgaca ggcactgaac gtgacccgca atgccgctcg cagcaaatcg ccacgctgga
    10381 agatgcgggg atcgcggtag tgagttcgct accggaagcc accttgctgg cagcggcgtt
    10441 aattcatccg ctctcgtcag ccacacagca acacacaccg tcattactgg aaaacgtcgc
    10501 cgtgattaac atcggattac gcagctttgc gctggagcta caaagcgcca gcaaaccggt
    10561 tgtgcattac caatggtcgc cagtcgccgg cggcaataaa aaactggctc gtttattaga
    10621 acgtttgcaa taaggggttc ccatgtttac atcagtggcg caagccaatg ctgcggttat
    10681 cgaacaaatt cgtcgcgctc gtccacactg gctggatgtg caaccggctt cttcacttat
    10741 cagcgaacta aacaagggca aaacactgct tcacgccggg ccgccaatgc gctggcagga
    10801 gatgaccgga cccatgaaag gggcgtgcgt gggcgcatgt ctgttcgaag gctgggcgaa
    10861 agatgaagcg caggcgctgg caatactgga gcagggggaa gtgaacttca ttccttgtca
    10921 ccatgtaaat gccgtcgggc caatgggcgg tattacttct gccagtatgc cgatgctggt
    10981 ggtcgagaac gtgaccgacg gcaacctggc gtactgcaac ctcaacgaag gtatcggcaa
    11041 agtgatgcgt tttggcgctt acggcgaaga tgtcctgact cgccatcgct ggatgcgcga
    11101 tgtgttaatg ccagtattaa gcgcggcgct ggggcgcatg gagcgcggta tcgatctcac
    11161 ggcaatgatg gcgcagggca ttaccatggg cgatgagttc catcaacgca atattgcttc
    11221 ctctgcactg ttaatgcgtg cgctggcccc acaaattgct cgcctcgatc atgataaaca
    11281 gcacattgcg gaagtgatgg atttcctcag cgtgaccgat cagttcttcc tcaatctcgc
    11341 gatggcctac tgcaaggcgg cgatggatgc aggcgcgatg atccgcgcag gcagcatcgt
    11401 tacggctatg acccgcaacg gcaatatgtt cgggattcgg gtaagcgggc tgggtgaacg
    11461 ctggtttact gcgcctgtaa acactccgca aggtctgttt ttcaccggct tctcgcagga
    11521 gcaggcgaac ccggatatgg gcgatagcgc gattaccgaa acctttggta ttggaggtgc
    11581 ggcaatgatc gcagcgcctg gcgtaacgcg ctttgtcggt gcgggtggca tggaagcggc
    11641 cagagcggta tctgaagaga tggcggaaat ttaccttgaa cgcaatatgc agttgcagat
    11701 ccccggctgg gatttccagg gggcgtgcct ggggctggac attcgtcgcg tggtagaaac
    11761 cggcattacg ccactcatca ataccggtat cgcccataaa gaggcgggga tcgggcagat
    11821 tggcgcaggc accgtgcggg caccgctggc gtgctttgaa caggcgctgg aagcactggc
    11881 tgaaagcatg gggattggtt gaggaacgca caatgacgat catccatcct ctacttgcca
    11941 gccgtagcgc accgaattat cgccagtcct ggcggttagc gggagtgtgg cggcgggcga
    12001 ttaacctgat gacggaaagc ggcgaactgt taacgctgca tcgtcagggg agtggtttcg
    12061 gccccggcgg atgggtgctt cgccgcgcgc aattcgatgc gttatgcggt ggattatgcg
    12121 gcaatgaacg accacaggtt gtggcacaag ggattcgcct cgggcgtttc acggttaaac
    12181 agccacagcg ttattgtttg ctacgtatta cgccgcctgc gcatcctcaa gcgcttgccg
    12241 ttgcatggat gcaacgcgcg gaggaaaccg ggcttttcgg gccactggca ttggcggcaa
    12301 gcgatccgct gcctgctgaa ttacgccagt ttcgtcactg ttttcaggcc gcgctcaatg
    12361 gcgttaagac cgactggcgg cactggctgg gtaaaggccc cggattaacg ccaagtcatg
    12421 atgacacgct gagcggaatg ctgctggcgg cctggtatta tggcgcttta gatgcgcgct
    12481 ccggtcgccc gttttttgcc tgttccgaca atcttcaact cgttaccact gcggtgagcg
    12541 tcagttattt acgttatgcc gcgcaaggat atttcgcctc gccactcctg cactttgttc
    12601 atgctctgag ttgcccgaaa cgtaccgcag cggcgatcga ttcgctgctg gcgctggggc
    12661 atacgtcagg ggccgatacg ctgctggggt tctggcttgg ccaacaatta ttacaaggaa
    12721 aaccatgaaa acactggttg tggctcttgg gggcaacgcc ttactccagc gcggtgaggc
    12781 gctgacggca gaaaatcaat atcgcaatat tgccagtgct gtgcccgcgc tggcgcgcct
    12841 ggcccgttct tatcggctgg cgattgttca cggcaacggg ccgcaggtgg ggctgctggc
    12901 gttacagaat ctggcgtgga aagaggtaga accgtatccg ctggatgtac tggtggcgga
    12961 aagccagggg atgattggct atatgctggc gcagagcttg agcgcacagc cgcagatgcc
    13021 gcctgtcacg acggtgctga cgcgcattga ggtttcgcct gatgatccgg cgtttttgca
    13081 gccagagaag tttattggtc cggtttatca gccagaagaa caagaggcac tggaagcggc
    13141 ttacggctgg cagatgaaac gtgatggtaa atatttacgc cgggtggtgg cgtctccgca
    13201 accgcgtaaa attctcgaca gcgaagccat cgagttgttg ctcaaagagg ggcatgtggt
    13261 gatttgcagt ggcggcggcg gtgtgcctgt gacggaagac ggggcaggga gtgaagcggt
    13321 gattgataaa gatcttgccg ctgcgttgct cgccgagcag attaatgccg atggactggt
    13381 gatcctcacc gatgctgatg cggtatatga aaactgggga acgccgcagc aacgtgccat
    13441 tcgccatgcc acaccggatg agttagcgcc atttgccaaa gccgatggtt cgatggggcc
    13501 gaaggtaacg gcggtgagtg gctatgtcag aagccgtggt aaacccgcgt ggattggggc
    13561 gttatcgcgg attgaagaga cgctggcggg cgaagcgggg acttgtattt cgctgtagtg
    13621 atgggcacca ggcaattggc acatctagcc tgatgcgatg ctggcgcgtc ttatcaggtc
    13681 tacaggcggt gtcggaacgg taggccggat aaggc
//
LOCUS       DADSYM010000132        13600 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2415
            SAMN08039579-rid11723173.denovo.133, whole genome shotgun sequence.
ACCESSION   DADSYM010000132 DADSYM010000000
VERSION     DADSYM010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039579
            Sequence Read Archive: SRR6362533
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13600)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13600)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980162.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 03:39:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,484
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 4,347
            CDSs (with protein)               :: 4,347
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 43 of 85
            Pseudo Genes (incomplete)         :: 37 of 85
            Pseudo Genes (internal stop)      :: 19 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13600
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039579-rid11723173.denovo.133"
                     /strain="LGE2415"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8302 N 95.9628 W"
                     /collection_date="2015-08-24"
     gene            <1..1330
                     /gene="chbC"
                     /locus_tag="J1Y83_003427"
     CDS             <1..1330
                     /gene="chbC"
                     /locus_tag="J1Y83_003427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIC"
                     /protein_id="HBA7631543.1"
                     /translation="VLLPFAVKIGKQPHVNAIKNGFIRLMPLTLAGAMFVLINNVFLS
                     FGEGAFFYSLGIRLDASTIETLNGLKGIGGNVYNGTLGIMSLMAPFFIGMALAEERKV
                     DALAAGLLSVAAFMTVTPYSVGEAYAVGANWLGGANIISGIIIGLVVAEMFTFIVHRN
                     WVIKLPDSVPTSVSRSFSALIPGFIILSVMGIIAWALNTWGTNFHQIIMDTISTPLAS
                     LGSVVGWAYVIFVPLLWFFGIHGALALTALDNGIMTPWALENIATYQQYGSVEAALAA
                     GKTFHIWAKPMLDSFIFLGGSGATLGLILAIFIASRRADYRQVAKLALPSGIFQINEP
                     ILFGLPIIMNPVMFIPFVLVQPILAAITLAAYYMGIIPPVTNIAPWTMPTGLGAFFNT
                     NGSVAALLVALFNLGIATLIYLPFVVVANKAQNAIDKEESEEDIANALKF"
     gene            1381..1731
                     /gene="chbA"
                     /locus_tag="J1Y83_003428"
     CDS             1381..1731
                     /gene="chbA"
                     /locus_tag="J1Y83_003428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIA"
                     /protein_id="HBA7631544.1"
                     /translation="MMDLNNIPDTQTEAEELEEVVMGLIINSGQARSLAYAALKQAKQ
                     GDFAAAKAMMDQSRMALNEAHLVQTKLIEGDAGEGKMKVSLVLVHAQDHLMTSMLARE
                     LITELIELHEKLKA"
     gene            1742..2581
                     /gene="chbR"
                     /locus_tag="J1Y83_003429"
     CDS             1742..2581
                     /gene="chbR"
                     /locus_tag="J1Y83_003429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310468.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ChbR"
                     /protein_id="HBA7631545.1"
                     /translation="MQPVINAPEIATAREQQLFNGKNFHVFIYNKTESISGLHQHDYY
                     EFTLVLTGRYFQEINGKRVLLERGDFVFIPLGSHHQSFYEFGATRILNVGISKRFFEQ
                     HYLPLLPYCFVASQVYRTNNAFLTYVETVISSLNFRETGLEEFVEMVTFYVINRLRHY
                     REEQVIDDIPQWLKSTVEKMHDKEQFSESALENMVTLSAKSQEYLTRATQRYYGKTPM
                     QIIYEIRINFAKKQLEMTNYSVTDIAFEAGYSSPSLFIKTFKKLTSFTPKSYRKKLTE
                     FNQ"
     gene            2684..4036
                     /locus_tag="J1Y83_003430"
     CDS             2684..4036
                     /locus_tag="J1Y83_003430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HBA7631546.1"
                     /translation="MSQKLKVVTIGGGSSYTPELLEGFIKRYHELPVSELWLVDVEGG
                     KAKLDIIFDLCQRMIDNAGVPMKLYKTLDRREALKDADFVTTQLRVGQLPARELDERI
                     PLSHGYLGQETNGAGGLFKGLRTIPVIFDIVKDVEELCPNAWVINFTNPAGMVTEAVY
                     RHTGFKRFIGVCNIPIGMKMFIRDVLMLKDSDDLSIDLFGLNHMVFIKDVLVNGKSRF
                     AELLDGVASGQLKASGVKNIFDLPFSEGLIRSMNLLPCSYLLYYFKQKEMLAIEMGEY
                     YKGGARAQVVQKVEKQLFELYKNPELNVKPKELEQRGGAYYSDAACEVINAIYNDKQA
                     EHYVNIPHHGHIDNIPADWAVEMTCTLGRDGATPHPRITHFDDKVMGLIHTIKGFEIA
                     ASNAALSGEFNDVLLALNLSPLVHSDRDAELLAREMILAHEKWLPNFADCIAELKKAH
                     "
     gene            4049..4807
                     /gene="chbG"
                     /locus_tag="J1Y83_003431"
     CDS             4049..4807
                     /gene="chbG"
                     /locus_tag="J1Y83_003431"
                     /EC_number="3.5.1.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chitin disaccharide deacetylase"
                     /protein_id="HBA7631547.1"
                     /translation="MERLLIVNADDFGLSKGQNYGIIEACRNGIVTSTTALVNGQAID
                     HAVQLSRDEPSLAIGMHFVLTMGKPLTAMPGLTRDGVLGKWIWQLAEEDALPLEEITQ
                     ELASQYLRFIELFGRKPTHLDSHHHVHMFPQIFPIVAKFVAEEGIALRIDRQPLSNDG
                     DLPANLRSSQGFSSAFYGEEISEALFLQVLDDSSHRGERSLEVMCHPAFVDNTIRQSA
                     YCFPRLTELHVLTSASLKYAIAERGYRLGSYHDV"
     gene            complement(4854..7115)
                     /gene="katE"
                     /locus_tag="J1Y83_003432"
     CDS             complement(4854..7115)
                     /gene="katE"
                     /locus_tag="J1Y83_003432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catalase HPII"
                     /protein_id="HBA7631548.1"
                     /translation="MSQHNEKNPHQHQSPLHDSSEAKPGMDSLAPEDGSHRPAAEPTP
                     PGAQPTAPGSLKAPDTRNEKLNSLEDVRKGSENYALTTNQGVRIADDQNSLRAGSRGP
                     TLLEDFILREKITHFDHERIPERIVHARGSAAHGYFQPYKSLSDITKADFLSDTNKIT
                     PVFVRFSTVQGGAGSADTVRDIRGFATKFYTEEGIFDLVGNNTPIFFIQDAHKFPDFV
                     HAVKPEPHWAIPQGQSAHDTFWDYVSLQPETLHNVMWAMSDRGIPRSYRTMEGFGIHT
                     FRLINAEGKATFVRFHWKPLAGKASLVWDEAQKLTGRDPDFHRRELWEAIEAGDFPEY
                     ELGFQLIPEEDEFKFDFDLLDPTKLIPEELVPVQRVGKMVLNRNPDNFFAENEQVAFH
                     PGHIVPGLDFTNDPLLQGRLFSYTDTQISRLGGPNFHEIPINRPTCSYHNFQRDGMHR
                     MGIDTNPANYEPNSINDNWPRETPPGPKRGGFESYQERVEGNKVRERSPSFGEYYSHP
                     RLFWLSQTPFEQRHIVDGFSFELSKVVRPYIRERVVDQLAHIDLTLAQAVAKNLGIEL
                     TDDQLNITPPPDVNGLKKDPSLSLYAIPDGDVKGRVVAILLNDEVRSADLLAILKALK
                     AKGVHAKLLYSRMGEVSADDGTVLSIAATFAGAPSLTVDAVIVPCGNIADIANNGDAN
                     YYLMEAYKHLKPIALAGDARKFKATIKVADQGEEGIAEADCASGSFMDELLTLMTAHR
                     VWSRIPKIDKIPA"
     gene            7298..7561
                     /gene="cedA"
                     /locus_tag="J1Y83_003433"
     CDS             7298..7561
                     /gene="cedA"
                     /locus_tag="J1Y83_003433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division activator CedA"
                     /protein_id="HBA7631549.1"
                     /translation="MRLVKPVMKKPLRQQNRQIISYVPRTEPAPPEHAIKMDSFRDVW
                     MLRGKYVAFVLMGESFLRSPAFTVPESAQRWANQIRQENEVEE"
     gene            complement(7608..8999)
                     /locus_tag="J1Y83_003434"
     CDS             complement(7608..8999)
                     /locus_tag="J1Y83_003434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine transporter"
                     /protein_id="HBA7631550.1"
                     /translation="MNFPLIANIVVFVVLLFALAQTRHKQWSLAKKVLVGLVMGVVFG
                     LALHTIYGSDSQVLKDSVQWFNIVGNGYVQLLQMIVMPLVFASILSAVARLHNASQLG
                     KISFLTIGTLLFTTLIAALVGVLVTNLFGLTAEGLVQGGAETARLNAIESNYVGKVSD
                     LSVPQLVLSFIPKNPFADLTGANPTSIISVVIFAAFLGVAALKLLKDDAPKGERVLTA
                     IDTLQSWVMKLVRLVMQLTPYGVLALMTKVVAGSNLQDIIKLGSFVVASYLGLLIMFA
                     VHGLLLGINGVSPLKYFRKVWPVLTFAFTSRSSAASIPLNVEAQTRRLGVPESIASFA
                     ASFGATIGQNGCAGLYPAMLAVMVAPTVGINPLDPMWIATLVGIVTVSSAGVAGVGGG
                     ATFAALIVLPAMGLPVTLVALLISVEPLIDMGRTALNVSGSMTAGTLTSQWLKQTDKA
                     ILDSEDDAELAHR"
     gene            complement(9132..9722)
                     /locus_tag="J1Y83_003435"
     CDS             complement(9132..9722)
                     /locus_tag="J1Y83_003435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310461.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HBA7631551.1"
                     /translation="MTAEGHLLFSIACAVFAKNAELTPVLAQGDWWHIVPSAILTCLL
                     PDIDHPKSFLGQRLKWISKPIARAFGHRGFTHSLLAVFALLATFYLKVPESWFIPADA
                     LQGMVLGYLSHILADMLTPAGVPLLWPCRWRFRLPILVPQKGNQLERFICMALFVWSV
                     WMPHSLPENSAVRWSSQMINTLQIQFHRLIKHQVEY"
     gene            complement(9886..10554)
                     /gene="hxpB"
                     /locus_tag="J1Y83_003436"
     CDS             complement(9886..10554)
                     /gene="hxpB"
                     /locus_tag="J1Y83_003436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexitol phosphatase HxpB"
                     /protein_id="HBA7631552.1"
                     /translation="MSTPRQILAAIFDMDGLLIDSEPLWDRAELDVMASLGVDISRRN
                     ELPDTLGLRIDMVVDLWYARQPWNGPSRQEVVEQVIARAISLIEETRPLLPGVREAVA
                     LCKEQGLLVGLASASPLHMLEKVLTMFDLRDSFDALASAEKLPYSKPHPQVYLDCAAK
                     LGVDPLTCVALEDSVNGMIASKAARMRSIVVPAPEAQNDPRFVLANVKLSSLTELTAK
                     DLLG"
     gene            10701..11237
                     /locus_tag="J1Y83_003437"
     CDS             10701..11237
                     /locus_tag="J1Y83_003437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA7631553.1"
                     /translation="MTYQQAGRIAVLKRILGWVIFIPALISTLISLLKFMNTRQENQE
                     GINAVMLDFTHVMIDMMQANTPFLNLFWYNSPTPNFNGGVNVMFWVIFILIFVGLALQ
                     DSGARMSRQARFLREGVEDQLILEKAKGEEGLTREQIESRIVVPHHTIFLQFFSLYIL
                     PVICIAAGYVFFSLLGFI"
     gene            complement(11278..12138)
                     /locus_tag="J1Y83_003438"
     CDS             complement(11278..12138)
                     /locus_tag="J1Y83_003438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HBA7631554.1"
                     /translation="MWQAISRLLSEQLGEGEIELRNELPGGEVHAAWHLRYAGRDFFV
                     KCDERELLPGFTAEADQLELLSRSKTVTVPKVWAVGADRDYSFLVMDYLPPRPLDAHN
                     AFILGQQIARLHQWSDQPQFGLDFDNALSTTPQPNTWQRRWSTFFAEQRIGWQLELAA
                     EKGIAFGNIDAIVEHIQQRLASHQPQPSLLHGDLWSGNCALGPDGPYIFDPACYWGDR
                     ECDLAMLPLHTEQPPQIYDGYQSVSPLPADFLERQPVYQLYTLLNRARLFGGQHLVIA
                     QQSLDRLLAA"
     gene            complement(12244..12534)
                     /gene="ghoS"
                     /locus_tag="J1Y83_003439"
     CDS             complement(12244..12534)
                     /gene="ghoS"
                     /locus_tag="J1Y83_003439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type V toxin-antitoxin system endoribonuclease
                     antitoxin GhoS"
                     /protein_id="HBA7631555.1"
                     /translation="MASGDLVRYVITVMLHEDTLTEINELNNYLTRDGFLLTMTDDDG
                     NIHELGTNTFGLISTQSEEEIRELVSGLTHSATGKDPEITITTWEEWNSNRK"
     gene            complement(12635..13564)
                     /gene="pfkB"
                     /locus_tag="J1Y83_003440"
     CDS             complement(12635..13564)
                     /gene="pfkB"
                     /locus_tag="J1Y83_003440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416237.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase II"
                     /protein_id="HBA7631556.1"
                     /translation="MVRIYTLTLAPSLDSATITPQIYPEGKLRCTAPVFEPGGGGINV
                     ARAIAHLGGTATAIFPAGGATGEHLVSLLADENVPVATVEAKDWTRQNLHVHVEASGE
                     QYRFVMPGAALNEDEFRQLEEQVLEIESGAILVISGSLPPGVKLEKLTQLISAAQKQG
                     IRCIVDSSGEALSAALAIGNIELVKPNQKELSALVNRELTQPDDVRKAAQEIVNSGKA
                     KRVVVSLGPQGALGVDSENCIQVVPPPVKSQSTVGAGDSMVGAMTLKLAENASLEEMV
                     RFGVAAGSAATLNQGTRLCSHDDTQKIYAYLSR"
BASE COUNT         3418 a         3211 c         3474 g         3497 t
ORIGIN      
        1 ggtactcctc ccttttgcag ttaaaatagg aaagcagcca cacgttaatg caatcaaaaa
       61 tggctttatt cgcttaatgc cgttaaccct tgcgggggcc atgtttgtat taattaacaa
      121 cgtttttcta agctttgggg agggggcgtt tttttattcc ttaggtattc gcctggatgc
      181 ctcaaccatt gaaacactta atggtctgaa aggtattggc ggcaacgtat ataacggaac
      241 attagggata atgtctttaa tggccccgtt ctttattggc atggcgctgg cagaagagcg
      301 taaagtcgat gcgctggcgg ccgggttgtt atccgttgca gcatttatga ccgtcacccc
      361 atatagtgtc ggtgaggcct atgcggttgg cgcaaactgg ttaggtgggg cgaatatcat
      421 ctccgggatt attattggcc tggtggtggc agaaatgttt acctttattg ttcatcgtaa
      481 ctgggtcatt aagttacctg atagcgttcc gacatcggtt tcgcgctctt tctcagcatt
      541 aattcccggt tttattattc tttccgtgat ggggattatt gcctgggcgt tgaatacctg
      601 gggcaccaac ttccatcaga tcattatgga taccatctca accccactgg catcgttggg
      661 tagcgtggta ggctgggcct atgtgatctt tgttccactg ctctggttct tcggtattca
      721 tggtgcgctg gcgctgaccg cactggacaa cggcattatg acgccgtggg cgctggaaaa
      781 tatcgcgacc tatcagcaat atggttccgt cgaagcggcg ctggcagccg gtaagacctt
      841 ccatatctgg gccaagccga tgctggactc ctttattttc cttggtggca gtggtgcgac
      901 tttaggcctg atcctggcta tctttatcgc ttctcgccgt gctgattatc gtcaggtggc
      961 aaaactggcg ctgccgtccg gcatcttcca gattaacgaa ccgattctgt ttggtctgcc
     1021 aattatcatg aacccggtga tgtttatccc gtttgtactg gtacaaccga ttctggcggc
     1081 aatcaccctg gcagcgtact acatgggcat tattccaccg gtgaccaata ttgcaccgtg
     1141 gaccatgcca accggtctgg gagccttctt taacaccaac ggtagcgtcg ccgcattgct
     1201 ggtcgcactc ttcaatcttg gtatcgcaac gttaatttat ctgccctttg ttgtggtcgc
     1261 taacaaagca caaaacgcga ttgataaaga agagagcgaa gaagatatcg ccaacgccct
     1321 gaaattttaa ttgatgccgg tacgggtaat cgtgccggta agaatagaga ggaacgatgt
     1381 atgatggatc tcaataatat tcccgatacg caaacggaag ctgaagagct ggaagaagtg
     1441 gtgatggggc ttatcatcaa ctccggacaa gcgcgtagcc tggcgtatgc ggcactgaaa
     1501 caggcgaagc agggcgattt tgccgcagca aaagcgatga tggatcagtc acggatggca
     1561 ttgaatgaag cgcatctggt acagacgaaa ctgattgaag gcgatgctgg cgaaggtaag
     1621 atgaaagtga gtctggtgct ggtccacgct caggatcatt taatgacgtc catgcttgcg
     1681 cgtgaactga ttactgaatt aattgagctt catgaaaaac tgaaggcata aggagtcgat
     1741 gatgcagcca gtgattaacg cgccggaaat tgccactgcc cgagaacagc agttgtttaa
     1801 tggcaaaaac ttccatgtgt ttatctacaa taaaactgag agtatcagcg gactgcatca
     1861 gcacgactat tatgaattta ctctggtatt aaccgggcgt tatttccagg agattaacgg
     1921 taagcgcgtg ttactggaac ggggcgattt tgtttttatt ccgttaggtt cgcaccatca
     1981 aagtttttat gaatttggtg ccacgcgtat attgaacgtt gggatcagta aacgcttttt
     2041 tgagcagcat tacctgccgt tgttgcctta ttgctttgta gcttcgcagg tataccggac
     2101 caataacgct tttctcacct atgtggaaac agtgatttct tcattgaatt tccgcgaaac
     2161 agggctggaa gagtttgttg agatggttac tttttatgtc attaaccgtt tacgtcatta
     2221 ccgcgaagaa caggtgattg atgatatacc gcagtggctg aaaagtacgg tagaaaagat
     2281 gcatgataaa gagcagttta gtgaatcggc gctggagaat atggtgacgt tgtcagccaa
     2341 atcacaggaa tatttgacgc gagcgactca acgatactat ggtaaaacgc caatgcagat
     2401 tatttatgaa atccgtatta attttgccaa aaaacaactg gaaatgacca actattcagt
     2461 gacggatatt gcgtttgagg ccggttatag tagcccgagc ttgtttatta aaacgtttaa
     2521 gaaattaaca tcctttacgc ctaagagcta tcgtaagaaa ttgactgaat ttaatcagta
     2581 agtcgttata cctgacaatt cacatatctg tcctgttgct ggattattta tgtccggggg
     2641 cagatatgcc agatatcagt attctgtact gaagggagaa attatgagcc agaaattaaa
     2701 agtcgtcact attggtggcg ggagcagcta taccccggag ttactggaag gatttattaa
     2761 gcgttatcac gaattgccgg tcagcgaatt atggctggtg gatgtcgaag gtggtaaagc
     2821 gaaactggat attatttttg atctctgcca acggatgatt gataacgctg gcgtcccgat
     2881 gaagctttat aaaacgctgg atcgccgcga agcattgaaa gatgctgatt tcgttactac
     2941 ccaactgcgc gttggccaat taccggcgcg ggaactggat gaacgtattc cattaagtca
     3001 tggttatctt ggtcaggaaa ctaacggcgc gggcggtctg tttaaaggtc tgcgtaccat
     3061 tccggtgatt tttgacatcg taaaagatgt cgaagaacta tgtccgaatg catgggtgat
     3121 taacttcact aacccggcgg gaatggtcac tgaagccgtt tatcgtcata ccggatttaa
     3181 acgctttatc ggcgtgtgta atattccgat cggcatgaag atgtttattc gcgatgttct
     3241 gatgctgaaa gacagcgatg atttatctat cgatctgttc ggcctcaacc atatggtgtt
     3301 cattaaggat gtgctggtaa atggcaaatc acgctttgcc gaattgcttg atggtgtggc
     3361 atccgggcag ttaaaagcat ctggcgttaa aaatattttc gatctgccat ttagcgaagg
     3421 cttaattcgt tctatgaatc tgttgccgtg ttcttatttg ctttattact tcaagcaaaa
     3481 agagatgctg gctattgaaa tgggcgaata ctacaaaggc ggcgcacgag cgcaggtagt
     3541 acagaaagtc gagaaacaac tttttgagct atataaaaac ccggagttga acgttaagcc
     3601 gaaagaactg gaacagcgcg gtggggctta ttattctgat gctgcgtgcg aagtgatcaa
     3661 cgctatctat aacgacaagc aagcagaaca ttacgttaat atcccgcatc atgggcatat
     3721 tgataatatt ccggcagact gggcggtaga aatgacctgt acgctggggc gcgatggcgc
     3781 gacgccacat ccgcgcatta cgcatttcga tgataaagtg atggggctga ttcacaccat
     3841 caaaggcttc gagattgctg ccagcaacgc cgcacttagt ggagaattta acgatgtctt
     3901 actggcgcta aaccttagtc cgttggtgca ttccgatcgc gatgctgagc tgctggcacg
     3961 cgagatgatt ctggcgcacg agaaatggct gccaaatttt gccgactgca tcgcagagct
     4021 taaaaaagca cattaaccga ggctgattat ggaacgctta ctgattgtta atgccgatga
     4081 ttttggctta agcaaaggtc agaactacgg cattatcgag gcctgtcgca atgggattgt
     4141 cacgtcgacg acggcgctgg tgaatgggca ggctattgac catgcggtgc agttgagccg
     4201 tgatgaaccg agtctggcca tagggatgca ctttgtcctt actatgggca agcctctgac
     4261 agctatgccg gggttaaccc gcgatggtgt gctgggaaaa tggatctggc agttggcaga
     4321 agaagatgct ttaccgctgg aagaaattac tcaggagctc gccagtcagt atttgcgttt
     4381 cattgagcta tttggacgca aacctacgca tcttgatagc catcatcatg tgcatatgtt
     4441 cccgcagatt ttcccgattg tggcaaagtt tgtggctgaa gaggggattg cgttgcgcat
     4501 cgaccgtcaa ccgctatcta acgatggtga tttaccggct aatctgcgca gttcgcaggg
     4561 attcagtagc gcgttttatg gtgaagagat tagcgaggca ttgttcctgc aagtgcttga
     4621 tgattccagc catcgtgggg aacgctcgtt ggaagttatg tgccatccgg cgtttgtcga
     4681 taatacgatt cgccagagtg cttattgctt ccctcggtta acggaactgc atgtgctgac
     4741 ttcagcgtcg ttgaaatatg cgattgctga gcgtggttat cggttgggta gttatcatga
     4801 tgtgtaagta aggtggtaag gtcactcccc agggggcgtg accttatcca tatttacgcc
     4861 gggattttgt caatcttagg aatgcgtgac cacacgcggt gtgctgtcat cagcgttaac
     4921 agttcatcca taaaactacc agaggcgcag tcagcttccg caatcccttc ttcaccctgg
     4981 tcagcgacct tgattgttgc tttaaacttg cgcgcgtctc ctgccagcgc aatcggttta
     5041 aggtgtttgt aggcttccat cagatagtag ttggcatcgc cgttgttagc gatatccgca
     5101 atattgccgc aagggacaat gactgcatcg accgtcagcg aaggcgcacc ggcaaaggta
     5161 gcagctatag acaacaccgt accgtcatcc gcactcactt cccccattcg ggagtagagc
     5221 agtttggcat gaacgccttt ggccttcagt gccttgagaa tggccagaag gtctgccgat
     5281 ctcacttcat cattaagcaa aattgctacc acgcgacctt tcacatcacc gtcaggaatg
     5341 gcgtacagac ttaaggatgg atcctttttc agaccgttga cgtccggagg tggggtgata
     5401 ttcagctggt catcagtcag ttcgataccg agatttttcg ccaccgcctg ggccagagtg
     5461 agatcaatat gcgccagctg gtcaacaacg cgctcacgaa tatacggacg aactactttg
     5521 cttaactcaa aactgaaacc atcgacaata tggcgctgtt cgaatggcgt ctgacttagc
     5581 cagaacagac gcggatggga ataatattcg ccaaacgatg gactgcgctc gcgaacttta
     5641 ttgccttcca cgcgttcctg gtatgattca aaaccgccgc gtttcggccc cggcggtgtt
     5701 tcgcgcggcc agttatcgtt gatcgagttc ggttcgtaat tcgccgggtt agtgtcgatc
     5761 cccatacgat gcatgccgtc acgctggaaa ttatggtaag agcaggtcgg gcggttaatc
     5821 ggaatttcat ggaaattcgg cccaccaaga cgactgattt gtgtatcggt ataagagaac
     5881 aaacgcccct gcaataacgg gtcgttggtg aaatccagac cggggacaat atgccctgga
     5941 tggaaagcca cctgttcgtt ttcagcaaag aagttatccg gattacgatt gagcaccatt
     6001 ttgccgacac gctgaacggg caccaattct tccgggataa gtttggttgg atcgagaaga
     6061 tcgaagtcga acttgaattc gtcttcttca ggaatcaact ggaagcccag ttcgtattca
     6121 ggaaaatcgc ctgcttcaat cgcttcccac aactcgcggc ggtggaagtc cgggtcacgt
     6181 ccggttaatt tttgtgcttc atcccaaacg agtgaggctt tacctgccag tggtttccag
     6241 tggaaacgta cgaacgttgc cttcccttcg gcattaatca ggcggaaggt atgaataccg
     6301 aagccttcca tagtgcggta actgcgcggg ataccgcgat ccgacattgc ccacatcacg
     6361 ttgtgcagag tttcaggttg cagagaaaca taatcccaga aagtatcgtg ggcgctttgc
     6421 ccttgtggaa ttgcccagtg cggttctggt tttaccgcat gaacaaaatc ggggaattta
     6481 tgtgcatcct ggataaagaa gattggcgtg ttattgccaa cgaggtcaaa aataccctct
     6541 tcagtataga acttggtggc aaagccacgg atatcacgta cggtatcggc agagccagcg
     6601 ccgccctgaa cggtagagaa cctaacaaat actggggtga ttttgttcgt atctgagagg
     6661 aaatccgctt tggtgatatc gcttaagctt ttatatggct ggaaataacc gtgagctgct
     6721 gatccgcgtg catgaacaat acgttccggg atgcgctcat ggtcaaagtg agtgattttc
     6781 tcgcgcagaa taaaatcttc cagcagagtt ggaccacggc taccggcacg cagtgagttt
     6841 tgatcgtcgg cgatgcgcac gccctgatta gtggtcagcg cataattttc actgcctttg
     6901 cgtacgtctt ccagagaatt aagtttttcg ttacgcgtat caggggcttt caggctccct
     6961 ggggcggtag gttgtgcacc aggcggtgtt ggttcagccg ctggacgatg agatccgtct
     7021 tcaggtgcca gtgagtccat ccccggtttc gcttcgctgg aatcgtgtag tggtgactgg
     7081 tgctgatgtg ggttcttttc gttatgttgc gacattgaac tcgtctcctt aaattattac
     7141 tgaaagggcc gctgttttgg tcaaaaaacc ctctaactat agaccaccag ccagatcccg
     7201 cttcggaaac tattctggaa acggtgttat gaaatacacg caaagcggga cgtacagggg
     7261 ctaaatcggc taaactacag tttttctgat ttttaatatg cgtttagtga agccagtaat
     7321 gaagaaaccg ctccgtcagc aaaaccgcca gattattagc tatgtcccac gcacggaacc
     7381 cgcgccgcca gaacatgcga taaagatgga ttcgtttcga gatgtctgga tgctgcgtgg
     7441 caaatatgtt gcgtttgtac tgatggggga gtcctttctg cgctcaccgg cgtttactgt
     7501 gcctgaatcc gcccagcgtt gggcaaatca gattcgtcag gaaaatgagg tcgaagaata
     7561 aaaaaacacc tgtcgggatg acaggcgttt aacgtaacaa atcaggatta acggtgtgcc
     7621 agttcggcgt cgtcttcgct atccagaatg gctttatcgg tttgcttcag ccactggctg
     7681 gtcagcgtgc cagcggtcat cgagccacta acgtttagcg ccgtacgacc catgtcgata
     7741 agcggttcaa cggagattaa caatgccacc agcgttaccg gcaggcccat cgcaggcagc
     7801 acaatcagtg cggcgaaagt tgcaccacca ccgacaccgg caacgcctgc ggaactaacg
     7861 gtgacaatac cgaccagcgt cgcaatccac atcgggtcca gtgggttaat gcccaccgta
     7921 ggcgcaacca tcaccgccag cattgccgga tacaaaccgg cacaaccgtt ctgaccaatg
     7981 gtcgcaccga aagaggcggc gaaactggcg atggattcag gaacacccag acgacgcgtt
     8041 tgtgcttcca cattcagtgg gatagacgca gcactggaac ggctggtaaa ggcaaacgtc
     8101 agtactggcc ataccttacg gaagtacttc agcgggctca cgccattaat gcccagcaga
     8161 aggccatgca ctgcaaacat aatcagcaga ccgaggtagg acgcaacaac gaaacttccc
     8221 agtttgatga tgtcttgcag gttagaacct gcaaccactt tggtcatcag cgccagaaca
     8281 ccgtaagggg tcaactgcat gaccaggcga accagtttca tcacccagct ttgcagggta
     8341 tcgatagcgg ttaagacgcg ttcacctttc ggcgcatcat ccttcagcag tttcagcgca
     8401 gctacgccga ggaatgcggc aaaaattacc acgctgataa ttgacgtcgg attggctccg
     8461 gtcagatcgg caaacgggtt tttcgggata aaggacaaga ccagctgcgg aacgctcagg
     8521 tcagagactt taccaacata gttactttcg atggcgttca gacgtgcagt ttctgcacca
     8581 ccctgaacca gaccttcagc cgtcaaacca aacaggttgg tgaccagcac accgaccagc
     8641 gctgcaatca gcgtggtaaa caaaagcgta ccgatggtca gaaaactgat tttgcctaac
     8701 tgagatgcgt tatgcagacg ggcaaccgcg ctcagaatag aggcgaagac tagcggcata
     8761 acgatcattt gcagcagttg aacatagccg ttaccaacaa tgttaaacca ctgtacagaa
     8821 tctttaagta cctggctgtc agaaccataa atggtatgca gggcaaggcc aaaaaccaca
     8881 cccatcacaa gacccaccag cacttttttc gccagactcc actgtttgtg gcgggtttga
     8941 gccagcgcaa acagcagtac aacgaacacc acgatgttcg caattaatgg aaagttcatc
     9001 cccgttctcc tgatcttatt atttctatca ctgcgtaaca gtcatagtgt tggcgcaaga
     9061 ttaacagaac tgtgagctgg ctcttatatt caaatggaat tgcttattcc agaaaggcga
     9121 tttttgcctt tttagtattc aacctgatgc ttaataagcc gatgaaactg gatctgtaag
     9181 gtattgatca tttgcgatga ccaacgaaca gcgctgtttt cgggtaatga atggggcatc
     9241 cataccgacc agacaaataa tgccatgcag ataaaacgtt ccagttggtt gcccttttgg
     9301 ggaaccagga taggcaagcg gaaacgccag cggcatggcc agagcagggg aacaccggcg
     9361 ggtgtcagca tatcggcaag tatgtggctc aaataaccca gcaccatacc ctgcaacgca
     9421 tcagccggaa tgaaccagct ttcaggaact ttaaggtaaa aggttgccag cagcgcaaat
     9481 accgccagca gactgtgggt aaaaccacga tgcccaaaag cgcgggcgat cggttttgat
     9541 atccacttta atcgctgccc aagaaacgac tttgggtgat cgatgtccgg taacaaacac
     9601 gtcaggattg cggaagggac aatatgccac cagtcaccct gtgccagcac gggcgtcagc
     9661 tcggcatttt tggcaaatac cgcacaagca atagaaaaga gaaggtgacc ttccgccgtc
     9721 atgataaaac tcactaaact gtcgatacgt acagtatagg gtttttatac agtgcacgga
     9781 agtggtgacg gtgtaactct ttctcttaaa ttggcgatta atccgtcagt tgaatgcaaa
     9841 ttcagataaa gaaccagggg cagtgtgatc actgccccct ggtcattaac cgagaaggtc
     9901 ttttgcggtg agttctgtca gcgatgagag tttgacgttt gctaatacaa aacgtggatc
     9961 attttgcgct tctggcgcag gaacgacgat ggaacgcatg cgggctgctt tagaggcgat
    10021 catgccattt accgaatctt ccagtgccac gcaggtcaga gggtcaaccc ccagttttgc
    10081 tgcgcagtcg agatatactt gcggatgcgg cttgctgtaa ggcaattttt cagccgaggc
    10141 gagggcatcg aaactgtcgc gtaaatcaaa catggtcaat actttttcca gcatatgcag
    10201 cggtgacgcg gaggccagcc ccaccaataa accttgttct ttgcataacg caacggcttc
    10261 acgcacacct ggcaataatg gacgtgtctc ttcaatcagt gaaatggcac gggcaataac
    10321 ttgttctact acttcctgac ggcttggccc attccatggt tgccgggcgt accaaagatc
    10381 gaccaccata tcgatgcgta aacctaaggt gtccggcagc tcgttacgac gggagatatc
    10441 cacccccagg cttgccatca catccagttc ggctcgatcc cataaaggtt ctgagtcgat
    10501 aagtaatcca tccatatcaa aaattgcagc aagaatctga cgcggggttg acattacaac
    10561 ctctccttta gagcattata tgtaaggtat taagccgtgc ttatgattaa gcgcctatca
    10621 tatcgccttt aaagaatggg cgaaaacaga tatctacagg taaactaagt gaaacgacgc
    10681 gtcttaatca aggagaactc atgacgtatc aacaagctgg acgcattgct gttttgaaaa
    10741 ggattttggg ttgggtgatt tttatacctg cattgatctc tacattgatt tctttgctga
    10801 aatttatgaa tacccggcag gaaaaccagg aaggcattaa tgcggtcatg ctcgacttta
    10861 ctcatgtcat gatcgatatg atgcaggcga atacgccttt tttgaatctg ttctggtaca
    10921 actctccgac gcccaatttt aatggtggcg tgaacgttat gttctgggtg attttcatcc
    10981 tgatttttgt cggactggcg ttgcaggatt ccggagcccg gatgagtcgc caggcgcgct
    11041 tcttgcggga aggtgttgaa gatcaactca ttctggaaaa agccaaaggg gaagaggggc
    11101 taacgcgtga acaaattgag tcacgtattg ttgttccgca ccatactatt ttcctgcagt
    11161 ttttctcgct gtatatcctg ccggttattt gtattgctgc aggttatgtg ttcttttctc
    11221 tgcttgggtt tatttaatca aaaggccgca cggagcggcc attatcctca acccatatca
    11281 tgctgctaat aatctatcca gtgactgctg agcaataacc aaatgctgac cgccaaataa
    11341 ccttgcacga tttaacagcg tgtagagttg gtaaaccggt tgacgttcaa ggaagtcggc
    11401 aggtagcggg gatactgact gatagccgtc atagatttgt ggcggctgtt cagtatgcag
    11461 cggtaacatc gccaggtcgc actctcggtc accccagtaa caggccgggt cgaaaatgta
    11521 cgggccatcc ggacccagcg cacagttgcc ggaccataaa tcgccgtgca acagagaagg
    11581 ctgcggttga tgcgaggcga gacgctgctg aatatgctcg acgatggcat cgatattgcc
    11641 gaaagcgatc cctttttctg ccgccagttc cagctgccag ccaatccgtt gttcagcaaa
    11701 aaaagttgac cagcgacgtt gccaggtgtt gggctgtgga gttgtagaga gcgcgttatc
    11761 gaaatcgagg ccaaattgtg gttggtcgct ccattgatgt aaacgcgcaa tttgctgacc
    11821 aagaataaat gcattatgcg catccagcgg gcgaggtggg agataatcca tcaccagaaa
    11881 actgtagtca cggtcagcgc caactgccca aaccttaggc acggtgacgg ttttactacg
    11941 cgacagtaac tccagttggt cggcttcggc ggtaaaaccg ggaagcagtt ccctttcatc
    12001 acatttgacg aaaaagtcac gtcctgcata gcgcaaatgc catgcggcat ggacttctcc
    12061 gccaggcagt tcattacgca gttcgatttc gccttcacct aactgctcgc tcaaaagacg
    12121 actgattgcc tgccacatga tgtctctccc atgttgtctg ccagatcata aggttagcgc
    12181 aagaatgcgg tgtaaaaaca cgagctaacg cacaccacag actgattatt gcccaaaaac
    12241 catttatttt ctgttgctat tccattcctc ccaggtcgtg atggtgattt caggatcttt
    12301 gccggttgca ctgtgggtaa gccccgaaac cagttctcta atttcttctt cactttgggt
    12361 actgataagt ccaaaagtgt tagtccccag ctcatggata ttgccatcat catccgtcat
    12421 ggtgagcaaa aaaccgtcgc gagtcaggta attattcaac tcgttaattt cagtcaatgt
    12481 atcctcatgc aacatgacgg ttattacata acggacaaga tcgccactgg ccatagttca
    12541 cccctttgtt atcacatgac ttttgagcat agtcggagaa acgcgttgcc gacaggttgg
    12601 tggtgattcc cccaatgctg ggggaatgtt tttgttagcg ggaaaggtac gcgtaaattt
    12661 tttgcgtatc gtcatgggag cacaggcgtg ttccctggtt gagtgtggct gcactccccg
    12721 cagctacgcc aaaacggacc atctcttcaa gagaggcatt ttctgccagt ttaagtgtca
    12781 tcgcgccgac catgctgtca ccagcgccaa cggtactctg gcttttcacc ggtggaggca
    12841 ccacctgaat gcagttttca ctatcaacgc ccagcgcccc ttgtggaccc agggaaacga
    12901 caacccgttt ggccttgccg ctattaacga tttcctgcgc ggctttgcgg acatcgtctg
    12961 gctgggtgag ttcgcgattt accagcgcac tgagttcttt ttggttaggc ttaaccaact
    13021 cgatgttacc aatcgccagt gctgcactta acgcttcgcc agaactgtcg acgatgcagc
    13081 ggatcccttg tttttgcgca gcggaaatca gttgggttaa tttttccagc ttcacacctg
    13141 gcggcaggct tccgcttatg accaggatgg ccccggattc aatttccaga acttgctctt
    13201 cgagctggcg aaactcatct tcatttaatg ccgcgcctgg cataacaaaa cgatactgct
    13261 caccgcttgc ttccacatgt acgtgtaaat tctgccgggt ccagtctttg gcttctacag
    13321 tagcgacggg gacattttca tccgccaaca gtgaaaccag atgttcgccg gtcgcgccac
    13381 ccgccgggaa gatcgctgtg gcagtgcctc ccagatgggc aatggcgcgg gcgacattga
    13441 tgccgccgcc accgggttcg aacaccggtg cggtacagcg cagttttcct tcgggataaa
    13501 tttgcggggt aattgttgcg ctatcaagag agggcgcaag tgtcaacgta tagatacgta
    13561 ccatcatttc ctcctatagg ctgatttcag tctggcaccg
//
LOCUS       DADSYM010000133        13548 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2415
            SAMN08039579-rid11723173.denovo.134, whole genome shotgun sequence.
ACCESSION   DADSYM010000133 DADSYM010000000
VERSION     DADSYM010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039579
            Sequence Read Archive: SRR6362533
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13548)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13548)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980162.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 03:39:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,484
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 4,347
            CDSs (with protein)               :: 4,347
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 43 of 85
            Pseudo Genes (incomplete)         :: 37 of 85
            Pseudo Genes (internal stop)      :: 19 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13548
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039579-rid11723173.denovo.134"
                     /strain="LGE2415"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8302 N 95.9628 W"
                     /collection_date="2015-08-24"
     gene            complement(158..979)
                     /gene="bacA"
                     /locus_tag="J1Y83_003441"
     CDS             complement(158..979)
                     /gene="bacA"
                     /locus_tag="J1Y83_003441"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HBA7631557.1"
                     /translation="MSDMHSLLIAAILGVVEGLTEFLPVSSTGHMIIVGHLLGFEGDT
                     AKTFEVVIQLGSILAVVVMFWRRLFGLIGIHFGRPLQHEGESKGRLTLIHILLGMIPA
                     VVLGLLFHDTIKSLFNPINVMYALVVGGLLLIAAECLKPKEPRAPGLDDMTYRQAFMI
                     GCFQCLALWPGFSRSGATISGGMLMGVSRYAASEFSFLLAVPMMMGATALDLYKSWGF
                     LTTGDIPMFAVGFITAFVVALIAIKTFLQLIKRISFIPFAIYRFIVAAAVYVVFF"
     gene            complement(1070..1438)
                     /gene="folB"
                     /locus_tag="J1Y83_003442"
     CDS             complement(1070..1438)
                     /gene="folB"
                     /locus_tag="J1Y83_003442"
                     /EC_number="4.1.2.25"
                     /EC_number="5.1.99.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional dihydroneopterin
                     aldolase/7,8-dihydroneopterin epimerase"
                     /protein_id="HBA7631558.1"
                     /translation="MDIVFIEQLSVITTIGVYDWEQTIEQKLVFDIEMAWDNRKAAKS
                     DDVADCLSYADIAETVVSHVEGARFALVERVAEEVAELLLARFNSPWVRIKLSKPGAV
                     ARAANVGVIIERGNNLKENN"
     gene            1543..2160
                     /gene="plsY"
                     /locus_tag="J1Y83_003443"
     CDS             1543..2160
                     /gene="plsY"
                     /locus_tag="J1Y83_003443"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HBA7631559.1"
                     /translation="MSAIAPGMILIAYLCGSISSAILVCRLCGLPDPRTSGSGNPGAT
                     NVLRIGGKGAAVAVLIFDVLKGMLPVWGAYELGVSPFWLGLIAIAACLGHIWPVFFGF
                     KGGKGVATAFGAIAPIGWDLTGVMAGTWLLTVLLSGYSSLGAIVSALIAPFYVWWFKP
                     QFTFPVSMLSCLILLRHHDNIQRLWRRQETKIWTKFKRKREKDPE"
     gene            complement(2173..3105)
                     /gene="ttdR"
                     /locus_tag="J1Y83_003444"
                     /gene_synonym="dan"
     CDS             complement(2173..3105)
                     /gene="ttdR"
                     /locus_tag="J1Y83_003444"
                     /gene_synonym="dan"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator TtdR"
                     /protein_id="HBA7631560.1"
                     /translation="MLNSWPLAKDLQVLVEIVHSGSFSAAAATLGQTPAFVTKRIQIL
                     ENTLATTLLNRSARGVALTESGQRCYEHALEILTQYQRLVDDVTQIKTRPEGMIRIGC
                     SFGFGRSHIAPAITELMRNYPELQVHFELFDRQIDLVQDNIDLDIRINDAIPDYYIAH
                     LLTKNKRILCAAPEYLQKYPQPQSLQELSRHDCLVTKERDMTHGIWALGNGQEKKSVK
                     VSGHLSSNSGEIVLQWALEGKGIMLRSEWDVLPFLESGKLVRVLPEYAQSANIWAVYR
                     EPLYRSMKLRVCVEFLAAWCQQRLGKPDEGYQVM"
     gene            3312..4223
                     /gene="ttdA"
                     /locus_tag="J1Y83_003445"
     CDS             3312..4223
                     /gene="ttdA"
                     /locus_tag="J1Y83_003445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L(+)-tartrate dehydratase subunit alpha"
                     /protein_id="HBA7631561.1"
                     /translation="MMSESNKQQAVNKLTEIVANFTAMISTRMPDDVVDKLKQLKDAE
                     TSSMGKIIYHTMFDNMQKAIDLNRPACQDTGEIMFFVKVGSRFPLLGELQSILKQAVE
                     EATVKAPLRHNAVEIFDEVNTGKNTGSGVPWVTWDIVPDGDDAEIEVYMAGGGCTLPG
                     RSKVLMPSEGYEGVVKFVFENISTLAVNACPPVLVGVGIATSVETAAVLSRKAILRPI
                     GSRHPNPKAAELEVRLEEGLNRLGIGPQGLTGNSSVMGVHIESAARHPSTIGVAVSTG
                     CWAHRRGTLRVHADLTFENLSHTRSAL"
     gene            4220..4825
                     /gene="ttdB"
                     /locus_tag="J1Y83_003446"
     CDS             4220..4825
                     /gene="ttdB"
                     /locus_tag="J1Y83_003446"
                     /EC_number="4.2.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L(+)-tartrate dehydratase subunit beta"
                     /protein_id="HBA7631562.1"
                     /translation="MKKILTTPIKAEDLQDIRVGDVIYLTGTLVTCRDVCHRRLIELK
                     RPIPYDLNGKAIFHAGPIVRKNGDKWEMVSVGPTTSMRMESFEREFIEQTGVKLVVGK
                     GGMGPLTEEGCQKFKALHVIFPAGCAVLAATQVEEIEEVHWTELGMPESLWVCRVKEF
                     GPLIVSIDTHGNNLIAENKKLFAERRDPIVEEICEHVHYIK"
     gene            4873..6336
                     /locus_tag="J1Y83_003447"
     CDS             4873..6336
                     /locus_tag="J1Y83_003447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anion permease"
                     /protein_id="HBA7631563.1"
                     /translation="MKPSTEWWRYLAPLAVIAIIALLPLPAGLESHTWLYFAVFTGVI
                     VGLILEPVPGAVVAMVGISIIAILSPWLLFSPEQLAQPGFKFTAKSLSWAVSGFSNSV
                     IWLIFAAFMFGTGYEKTGLGRRIALILVKKMGHRTLFLGYAVMFSELILAPVTPSNSA
                     RGAGIIYPIIRNLPPLYQSQPNDSSSRSIGSYIMWMGIVADCVTSAIFLTAMAPNLLL
                     IGLMKSASHATLSWGDWFLGMLPLSILLVLLVPWLAYVLYPPVLKSGDQVPRWAETEL
                     QAMGPLCSREKRMLGLMVGALVLWIFGGDYIDAAMVGYSVVALMLLLRIISWDDIVSN
                     KAAWNVFFWLASLITLATGLNNTGFISWFGKLLAGSLSGYSPTIVMVALIVVFYLLRY
                     FFASATAYTSALAPMMIAAALAMPEIPLPVFCLMVGAAIGLGSILTPYATGPSPIYYG
                     SGYLPTADYWRLGAIFGLIFLVLLVITGLLWMPVVLL"
     gene            complement(6379..7392)
                     /gene="tsaD"
                     /locus_tag="J1Y83_003448"
     CDS             complement(6379..7392)
                     /gene="tsaD"
                     /locus_tag="J1Y83_003448"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001264383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HBA7631564.1"
                     /translation="MRVLGIETSCDETGIAIYDDEKGLLANQLYSQVKLHADYGGVVP
                     ELASRDHVRKTVPLIQEALKESGLTAKDIDAVAYTAGPGLVGALLVGATVGRSLAFAW
                     DVPAIPVHHMEGHLLAPMLEDNPPEFPFVALLVSGGHTQLISVTGIGQYELLGESIDD
                     AAGEAFDKTAKLLGLDYPGGPLLSKMAAQGTAGRFVFPRPMTDRPGLDFSFSGLKTFA
                     ANTIRDNGTDDQTRADIARAFEDAVVDTLMIKCKRALDQTGFKRLVMAGGVSANRTLR
                     AKLAEMMKKRRGEVFYARPEFCTDNGAMIAYAGMVRFKAGATADLGVSVRPRWPLAEL
                     PTA"
     gene            7630..7845
                     /gene="rpsU"
                     /locus_tag="J1Y83_003449"
     CDS             7630..7845
                     /gene="rpsU"
                     /locus_tag="J1Y83_003449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HBA7631565.1"
                     /translation="MPVIKVRENEPFDVALRRFKRSCEKAGVLAEVRRREFYEKPTTE
                     RKRAKASAVKRHAKKLARENARRTRLY"
     gene            7956..9701
                     /gene="dnaG"
                     /locus_tag="J1Y83_003450"
     CDS             7956..9701
                     /gene="dnaG"
                     /locus_tag="J1Y83_003450"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HBA7631566.1"
                     /translation="MAGRIPRVFINDLLARTDIVDLIDARVKLKKQGKNFHACCPFHN
                     EKTPSFTVNGEKQFYHCFGCGAHGNAIDFLMNYDKLEFVETVEELAAMHNLEVPFEAG
                     SGPSQIERHQRQTLYQLMDGLNTFYQQSLQQPVATSARQYLEKRGLSHEVIARFAIGF
                     APPGWDNVLKRFGGNPENRQSLIDAGMLVTNDQGRSYDRFRERVMFPIRDKRGRVIGF
                     GGRVLGNDTPKYLNSPETDIFHKGRQLYGLYEAQQDNAEPNRLLVVEGYMDVVALAQY
                     GINYAVASLGTSTTADHIQLLFRATNNVICCYDGDRAGRDAAWRALETALPYMTDGRQ
                     LRFMFLPDGEDPDTLVRKEGKEAFEARMEQAMPLSAFLFNSLMPQVDLSTPDGRARLS
                     TLALPLISQVPGETLRIYLRQELGNKLGILDDSQLERLMPKAAESGVSRPVPQLKRTT
                     MRILIGLLVQNPELATLVPPLENLDENKLPGLGLFRELVNTCLSQPGLTTGQLLEHYR
                     GTNNAATLEKLSMWDDIADRNIAEQTFTDSLNHMFDSLLELRQEELIARERTHGLSNE
                     ERLELWTLNQELAKK"
     gene            9896..11737
                     /gene="rpoD"
                     /locus_tag="J1Y83_003451"
     CDS             9896..11737
                     /gene="rpoD"
                     /locus_tag="J1Y83_003451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HBA7631567.1"
                     /translation="MEQNPQSQLKLLVTRGKEQGYLTYAEVNDHLPEDIVDSDQIEDI
                     IQMINDMGIQVMEEAPDADDLMLAENTADEDAAEAAAQVLSSVESEIGRTTDPVRMYM
                     REMGTVELLTREGEIDIAKRIEDGINQVQCSVAEYPEAITYLLEQYDRVEAEEARLSD
                     LITGFVDPNAEEDLAPTATHVGSELSQEDLDDDEDEDEEDGDDDTADDDNSIDPELAR
                     EKFAELRAQYIVTRDTIKAKGRSHAAAQEEILKLSEVFKQFRLVPKQFDYLVNSMRVM
                     MDRVRTQERLIMKLCVEQCKMPKKNFITLFTGNETSDTWFNAAIAMNKPWSEKLHDVS
                     EEVHRALQKLQQIEEETGLTIEQVKDINRRMSIGEAKARRAKKEMVEANLRLVISIAK
                     KYTNRGLQFLDLIQEGNIGLMKAVDKFEYRRGYKFSTYATWWIRQAITRSIADQARTI
                     RIPVHMIETINKLNRISRQMLQEMGREPTPEELAERMLMPEDKIRKVLKIAKEPISME
                     TPIGDDEDSHLGDFIEDTTLELPLDSATTESLRAATHDVLAGLTAREAKVLRMRFGID
                     MNTDHTLEEVGKQFDVTRERIRQIEAKALRKLRHPSRSEVLRSFLDD"
     gene            complement(11815..12321)
                     /gene="mug"
                     /locus_tag="J1Y83_003452"
     CDS             complement(11815..12321)
                     /gene="mug"
                     /locus_tag="J1Y83_003452"
                     /EC_number="3.2.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="G/U mismatch-specific DNA glycosylase"
                     /protein_id="HBA7631568.1"
                     /translation="MVEDILAPGLRVVFCGINPGLSSAGTGFPFAHPANRFWKVIYQA
                     GFTDRQLKPQEAQHLLDYRCGVTKLVDRPTVQANEVSKQELHAGGRKLIEKIEDYQPQ
                     ALAILGKQAYEQGFSQRGAQWGKQTLTIGSTQIWVLPNPSGLSRVSLEKLVEAYRELD
                     QALVVRGR"
     gene            12446..12521
                     /locus_tag="J1Y83_003453"
     tRNA            12446..12521
                     /locus_tag="J1Y83_003453"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:12479..12481,aa:Ile,seq:cat)
     gene            complement(12575..13339)
                     /gene="nfeF"
                     /locus_tag="J1Y83_003454"
     CDS             complement(12575..13339)
                     /gene="nfeF"
                     /locus_tag="J1Y83_003454"
                     /EC_number="1.16.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent ferric chelate reductase"
                     /protein_id="HBA7631569.1"
                     /translation="MNNTPRYPQRVRNDLRFRELTVLRVERISAGFQRIVLGGEALDG
                     FTSRGFDDHSKLFFPQPGTHFVPPTVTEEGIVWPEGPRPPSRDYTPLYDELRHELAID
                     FFIHDGGVASGWAMQAKPGDKLTVAGPRGSLVVPEDYAYQLYVCDESGMPALRHRLET
                     LSKLAVKPQVSALVSVRDNACQDYLAHLDGFNIEWLAHDEQAVDARLAQMQIPADDYF
                     IWITGEGKVVKNLSRRFEAEQYDPQRVRAAAYWHAK"
BASE COUNT         3288 a         3662 c         3463 g         3135 t
ORIGIN      
        1 gcgtcttatc cggctacatg tcaacgacag ttgtaggcct gataagacgc gcgagcgtcg
       61 catcaggcgt tgattgccgg atgcggcgtg aacgccttat ccggcctacg caatctgata
      121 agacgcgtca gcatcatatc cgacactgtt cttcacatca aaagaacaca acgtacaccg
      181 cagccgccac aataaagcga taaatggcga acgggataaa cgaaatgcgc ttaatcaatt
      241 gcaggaaggt tttaatcgct atcagcgcca ccacaaaagc ggtgataaac ccaacggcaa
      301 acatcgggat atcgccggta gtcaggaagc cccagctttt gtagagatcg agcgccgttg
      361 cgcccatcat catcggcacc gccagcagga acgagaactc ggaagcagcg taacggctca
      421 cccccatcag catcccacct gaaatggtcg ccccggaacg ggagaaaccc ggccacagcg
      481 ccagacactg gaaacagcca atcataaacg cctgacgata ggtcatatca tcaagacccg
      541 gcgcacgcgg ctctttcggc ttcaggcatt cggcggcaat cagcaacaga ccgccaacga
      601 ccagtgcata catgacattt atcgggttaa acaatgattt aatcgtgtcg tggaacaaca
      661 gccccagtac caccgccgga atcatcccca gcaagatgtg gatcagcgtt aaacgacctt
      721 tgctttctcc ttcgtgctgc aacgggcgac caaagtggat gccaatcagg ccaaacagac
      781 gccgccagaa catcactact accgccagaa ttgatcctaa ctggatcacg acttcaaagg
      841 ttttcgccgt gtcgccctca aaccccaaca agtgaccgac aataatcata tggcccgtgc
      901 tggataccgg cagaaattct gtcaatcctt cgaccacacc caatattgcc gctatcagca
      961 gcgagtgcat atcgctcatc aataaacccc taaattataa aaatgtaacg cttgtccgaa
     1021 ctactgcagt atgaccaggt tataatcgtt tggtttaaca gctgtaaaat taattatttt
     1081 ctttcagatt attgccacgc tcaatgatta cgccaacatt cgccgcccgc gccactgcgc
     1141 ctggcttgct gagtttgata cgcacccacg gcgagttgaa gcgtgctaac agcagctccg
     1201 ccacctcttc agccacgcgt tccaccagcg caaaacgcgc cccctcgacg tggctgacca
     1261 ccgtttctgc aatgtcagcg taactgaggc aatccgccac atcatcactt ttcgccgctt
     1321 tacggttatc ccacgccatt tcgatatcga acactaactt ctgttcgatg gtctgttccc
     1381 agtcgtaaac accaatagtg gtgattaccg aaagttgctc tataaataca atatccatca
     1441 cgtcctgcct gctttttggc taaccggata ccacttccgg cgaaatgtgc gtattatcca
     1501 cagattcatc gttgaacacg aattttcaaa acggaacagc ttatgagtgc aatcgcgcct
     1561 ggaatgatcc tcatcgcgta cctctgcggc tccatttcca gtgccattct ggtttgccgc
     1621 ttgtgtgggt tgcccgatcc gcgaaccagc ggctccggca atccaggcgc aaccaatgtg
     1681 ttacgtatcg gtggcaaggg agcagccgta gcagtactga ttttcgacgt tctgaaagga
     1741 atgttgcccg tctggggcgc gtatgaatta ggtgtcagcc ccttctggct aggcttaatt
     1801 gccatcgccg cctgtcttgg acacatctgg cccgttttct tcggttttaa aggaggaaaa
     1861 ggcgttgcta ccgcttttgg tgccatcgca ccaattggct gggatctcac tggagtaatg
     1921 gcgggaacct ggttactgac cgtgctattg agtggttact cgtcgctggg agcgattgtc
     1981 agtgcactga ttgctccgtt ttatgtctgg tggtttaagc cacaattcac cttcccggtt
     2041 tcgatgctct cttgcctgat cctgctgcgt catcatgaca acatccagcg tctgtggcgt
     2101 cgtcaggaga caaaaatctg gacgaaattc aaaagaaagc gcgaaaagga tcccgagtga
     2161 tttctggtgg atctacatga cctgatagcc ttcgtcgggc ttgcccagcc gttgctggca
     2221 ccatgccgcc agaaattcca cacagacacg taatttcatg ctgcgataga gcggctcccg
     2281 gtaaacagcc cagatattgg cgctttgtgc atactccggc aatactcgca ccagtttgcc
     2341 actctccaga aacggcaaca catcccactc ggaacgcagc ataatccctt tgccctccag
     2401 cgcccattgc agcacaatct cgccgctatt ggaggaaaga tgtccgctta ccttcaccga
     2461 ttttttctcc tgaccgttcc ccaacgccca tattccgtgg gtcatatcgc gctctttggt
     2521 caccaggcag tcatgacgac ttaattcctg taaggattgt ggctgcggat atttttgcag
     2581 atactcaggt gctgcgcata atattctttt attttttgtc aaaagatgcg caatataata
     2641 atcaggaatt gcgtcattaa tacgaatatc cagatcaata ttatcctgca ctaaatcaat
     2701 ttgtcgatcg aacaattcaa aatgcacctg taactcagga taattgcgca tcagttcggt
     2761 aatagctggc gcaatatgac tgcgcccaaa accaaaactg caaccaatgc ggatcatccc
     2821 ctccgggcgc gttttgattt gcgtgacgtc atcgaccagt cgctgatatt gggtgaggat
     2881 ttccagcgca tgttcgtaac aacgctggcc actttcagtc agcgccacgc cgcgagcgga
     2941 gcggttcagc agagttgttg ccagcgtatt ttcaaggatt tggatccgct tggtgacaaa
     3001 tgcaggcgtt tgcccaagcg tcgccgctgc agcactaaaa ctcccgctat ggacgatttc
     3061 caccagtacc tgcaagtctt tggctaaagg ccagctattt agcattgtta aatatccgca
     3121 gttttgataa acgcagtgta tttcgcaaaa cattaccatt ccctgctctg gcgcaatatt
     3181 cgttcttatt ttcgcgttga ttcacgaact gttaattaca tattcacggt ggcaaaaaat
     3241 ataaaaccac atttttagtg gtagtttgtg gcggtgaatt tttccagaca aatacataaa
     3301 ctggagttgc catgatgagc gaaagtaata agcaacaggc agtgaataag ttgacggaga
     3361 ttgtcgctaa ctttaccgcc atgatttcta cccgaatgcc tgatgacgtg gtggataaac
     3421 taaaacagct aaaggatgcc gaaacatcgt cgatgggaaa aatcatctac cacacgatgt
     3481 tcgataacat gcaaaaagcg atcgacctga atcgtcctgc ctgtcaggac accggcgaaa
     3541 tcatgttttt tgttaaggtc ggttcccgtt tcccactgct tggcgagctg caaagcatac
     3601 tcaaacaagc cgtggaagag gcaaccgtca aagcgccact gcgtcacaat gcggtagaaa
     3661 tttttgacga agtaaacacc ggcaaaaata ccggcagcgg cgtaccgtgg gtcacctggg
     3721 atatcgtccc cgacggtgac gatgcggaaa tcgaagttta catggcaggc ggcggctgca
     3781 cgctacctgg ccgatcgaaa gtgttaatgc cgtcagaagg ctacgaaggc gtagtgaaat
     3841 tcgtcttcga aaatatctcc accctcgccg taaacgcctg tccgccggta ctggtgggcg
     3901 ttggcatcgc tacctcggtg gaaaccgccg cagtgctctc gcgtaaagcc attttgcgcc
     3961 cgattggcag ccgccatccc aatccaaaag cggcagagct ggaagtacgc ctggaagaag
     4021 gactcaaccg tctggggatt ggtccacaag ggctgactgg caacagttca gtgatgggcg
     4081 tacatatcga atctgccgct cgccatccgt caaccatcgg cgttgctgtt tctaccggct
     4141 gctgggcgca tcgtcgcggc acactgcgag ttcatgccga tctcaccttt gaaaatctgt
     4201 ctcatacccg gagcgcgtta tgaaaaagat cctgacaacc ccgatcaaag ctgaagatct
     4261 gcaagatatt cgcgtcggcg atgtgatcta cctgaccggt acgctggtga cctgccgtga
     4321 cgtttgtcac cgccgattga tcgaactgaa acgtccgatc ccttacgatc tcaacggcaa
     4381 agcgattttc cacgctggcc ccatcgtgcg caaaaacggc gacaaatggg agatggtgtc
     4441 cgtcggcccg acaaccagta tgcgtatgga aagttttgaa cgtgaattta tcgagcagac
     4501 cggcgtgaaa ctggtggtcg gcaaaggcgg aatggggccg ctgaccgaag aaggctgcca
     4561 gaaattcaag gcgctgcatg tgattttccc ggctggctgt gcggtactgg cggcaactca
     4621 agtggaagag attgaagagg tacactggac agaactcggg atgcctgagt cgctgtgggt
     4681 ctgccgggtc aaagagtttg gcccgctgat tgtctctata gatacccacg gcaacaacct
     4741 gatagccgaa aacaaaaagc tgttcgccga acgccgcgat cccatcgtgg aagagatctg
     4801 cgagcacgtc cactacatca aataaccctc acggagaggc tcgcccctct cctttttcgc
     4861 aggcataaca cgatgaaacc ttccactgaa tggtggcgat accttgcgcc gctggcggtc
     4921 atcgccatta ttgctctact tccgcttccc gcagggctgg agagtcatac ctggctctac
     4981 tttgccgttt ttactggcgt gatcgttgga ctgatcctcg aacccgtgcc gggtgccgtg
     5041 gtggcgatgg tgggtatatc catcatcgcc attctttcgc cgtggctgct gtttagcccg
     5101 gagcagctcg cccagccagg ttttaagttc accgccaaat ccctctcctg ggcggtttct
     5161 ggtttttcta attcagttat ctggcttatt ttcgccgcct ttatgtttgg tacgggctat
     5221 gaaaaaaccg ggcttggacg tcgcatcgcg ctgattctgg tgaaaaaaat ggggcatcgc
     5281 acgctgtttc tcggctatgc agtgatgttc tccgagctga tactcgcgcc tgtaacaccg
     5341 tccaactcgg cgcgtggtgc ggggattatc tatcccatca tccgtaacct gccaccgctc
     5401 tatcaatcac aaccaaacga cagcagttcg cgcagcattg gctcgtacat catgtggatg
     5461 gggattgttg ccgactgcgt gaccagcgcc attttcctga cggcgatggc tccaaatttg
     5521 ctgttaattg gactgatgaa aagcgcatct cacgccacgc tgagctgggg cgactggttc
     5581 ctcgggatgt tgccgctcag cattttactg gttctgctgg ttccctggct ggcttacgtg
     5641 ctgtacccgc cggtactgaa gtctggcgat caggtgccgc gctgggcaga gacggaactg
     5701 caggcaatgg gcccgctctg ttcgcgtgaa aaacggatgc tggggctgat ggtaggcgca
     5761 ctggtgctgt ggatttttgg cggtgattat atcgatgccg cgatggtcgg ttacagcgtg
     5821 gtggcgctga tgctgcttct gcgcattatc agttgggacg acattgtcag taataaagcg
     5881 gcgtggaacg ttttcttctg gctggcctcg cttatcaccc tcgctaccgg actcaacaac
     5941 accggtttta ttagctggtt tggcaaactg ttagcaggca gcttaagcgg ttattcgcca
     6001 acgatagtga tggtggcgtt gattgtggtc ttttatctgc tgcgctactt ttttgccagc
     6061 gccacagcgt atacctccgc tctcgcaccg atgatgattg ccgccgcgct ggcgatgccg
     6121 gaaatcccgc tgccggtgtt ctgcctgatg gttggtgcgg caattggtct ggggagcatt
     6181 cttacaccat acgccaccgg acccagtccg atttactacg gcagtggtta tctgccaacg
     6241 gcggattact ggcgactggg ggcgattttt gggctgatat tcctcgtatt gctggtgatt
     6301 accggcttac tgtggatgcc cgtggtgttg ctttaagtga ggacggtgcc ggaagttgct
     6361 ctccggcacc taatagtttt acgcagtcgg taactccgcc agcggccagc gcggacgcac
     6421 actcacgccg agatccgccg tcgcgcctgc tttaaaccgc accattccgg cataggcgat
     6481 catcgcaccg ttatcagtgc aaaattccgg acgcgcgtag aacacttcgc cacgacgctt
     6541 tttcatcatt tccgccagtt tcgcccgcag tgtgcggtta gcactcacgc cgcccgccat
     6601 taccagacgc ttgaagcccg tctgatccag tgctcgctta cacttaatca tcaacgtatc
     6661 gaccactgca tcttcaaagg cgcgggcgat atcggcacgc gtctgatcgt cagtgccgtt
     6721 gtcacgtatg gtatttgccg cgaaggtttt cagaccagaa aagctgaaat ccagccccgg
     6781 acggtcggtc atcgggcgcg ggaagacaaa gcgcccggca gtaccctgag ccgccatttt
     6841 cgacagtaat ggcccgccag gataatccag ccccagcagc ttcgcggttt tatcaaacgc
     6901 ttcaccagcc gcatcatcga tagactctcc gagcagctcg tactgaccaa tgccagtcac
     6961 gctgattaac tgcgtatgac cgccggaaac cagcagcgcg acaaacggaa attccggtgg
     7021 gttatcttcc agcatcggcg ctaacagatg tccttccata tgatgtaccg ggattgccgg
     7081 aacgtcccag gcaaacgcca gagaacgccc cacggtcgcg ccaaccagca gtgcgccgac
     7141 taatcctggg cctgcggtat aggccacagc atcaatgtct tttgccgtta aaccagactc
     7201 ctttagcgcc tcctggatca acggtacggt tttacgcacg tgatcgcggg aggccagttc
     7261 aggcacgacg ccgccgtagt cagcgtgcaa tttcacctga ctatacaatt ggttggctaa
     7321 caaacctttt tcatcgtcgt aaatggcgat gccggtttca tcgcaggaag tttcaatacc
     7381 cagtacacgc atgacttgtt ttacctcgct ttattaccgc gcagtgtagg accaatgcgg
     7441 gttgatgtaa aactttgttc gcccctggag aaagcctcgt gtatactcct cacccttata
     7501 aaagtccctt tcaaaaaagg ccgcggtgct ttacaaagca gcagcaattg cagtaaaatt
     7561 ccgcaccatt ttgaaataag ctggcgttga tgccagcggc aaaccgaatt aatcaaaggt
     7621 gagaggcaca tgccggtaat taaagtacgt gaaaacgagc cgttcgacgt agctctgcgt
     7681 cgcttcaagc gttcctgcga aaaagcaggt gttctggcgg aagttcgtcg tcgtgagttc
     7741 tatgaaaaac cgactaccga acgtaagcgc gctaaagctt ctgcagtgaa acgtcacgcg
     7801 aagaaactgg ctcgcgaaaa cgcacgccgc actcgtctgt actaattccc cgagagcgtt
     7861 gctctccgat cagaccgagt tgtagttgta aggccgtgct tccgaaagga atgcgcggct
     7921 tattttcgtt tatgaattgc taaaaatcgg ggcctatggc tggacgaatc ccacgcgtat
     7981 tcattaatga tctgctggca cgcactgaca tcgtcgatct gatcgatgcc cgtgtgaagc
     8041 tgaaaaagca gggcaagaat ttccacgcgt gttgtccatt ccacaacgag aaaaccccgt
     8101 cattcaccgt taacggtgag aaacagtttt accactgctt tggatgtggc gcgcatggca
     8161 acgcgatcga cttcctgatg aactacgaca agctcgagtt cgtcgaaacg gtcgaagagc
     8221 tggcagcaat gcacaatctt gaagtgccat ttgaagcagg cagcggcccc agccagatcg
     8281 aacgccatca gcggcaaacg ctttatcagt tgatggacgg tctgaatacg ttttaccaac
     8341 aatctttaca acaacctgtt gccacgtctg cgcgccagta tctggaaaaa cgcggattaa
     8401 gccacgaggt tatcgcccgc tttgcgattg gttttgcgcc ccccggctgg gacaacgtcc
     8461 tgaagcggtt tggcggcaat ccagaaaatc gccagtcatt gattgatgcg ggcatgttgg
     8521 tcactaacga tcaggggcgc agttacgatc gtttccgcga gcgggtgatg ttccccattc
     8581 gcgataaacg cggtcgggtg attggttttg gcgggcgcgt gctgggcaac gatactccca
     8641 aatacctgaa ctcgccggaa acggacattt tccataaagg ccgccagctt tacggtcttt
     8701 atgaagcgca gcaggataac gctgaaccca atcgtctgct tgtggtcgaa ggctatatgg
     8761 acgtggtggc gctggcgcaa tacggcatta attacgccgt tgcgtcgtta ggtacgtcaa
     8821 ccaccgccga tcacatacaa ctgttgttcc gcgcgaccaa caatgtcatt tgctgttatg
     8881 acggcgaccg tgcaggccgc gatgccgcct ggcgagcgct ggaaacggcg ctgccttaca
     8941 tgacagacgg tcgtcagcta cgctttatgt ttttgcctga tggcgaagat cctgacacgc
     9001 tggtacgaaa agaaggtaaa gaagcgtttg aagcgcggat ggagcaggcg atgccactct
     9061 ccgcatttct gtttaacagt ctgatgccgc aagttgatct gagtacccct gacgggcgcg
     9121 cacgtttgag tacgctggca ctgccactga tatcgcaagt gccgggcgaa acgctgcgaa
     9181 tatatctacg tcaggaatta ggcaacaaat taggcatact tgatgacagc cagcttgaac
     9241 gattaatgcc aaaagcggca gagagcggcg tttctcgccc tgttccgcag ctaaaacgca
     9301 cgaccatgcg tatacttata gggttgctgg tgcaaaatcc agaattagcg acgttggtcc
     9361 cgccgcttga gaatctggat gaaaataagc tccctgggct tggcttattc agagaactgg
     9421 tcaacacttg tctctcccag ccaggtctga ccaccgggca acttttagag cactatcgtg
     9481 gtacaaataa tgctgccacc cttgaaaaac tgtcgatgtg ggacgatata gcagatagga
     9541 atattgctga gcaaaccttc accgactcac tcaaccatat gtttgattcg ctgcttgaac
     9601 tgcgccagga agagttaatc gctcgtgagc gcacgcatgg tttaagcaac gaagaacgcc
     9661 tggagctctg gacattaaac caggagctgg cgaaaaagtg atttaacggc ttaagtgccg
     9721 aagagcgatc gggaagcccc cgacagccgc actgagaggc agcggcaaat atataagtac
     9781 gccctcgtaa ttatcgttgg cggtaaacaa ccgttggatt tcagcgttaa cagctgaagg
     9841 acatcgggtc aatcgcccaa caccaacctc atgaaataag tgtggatacc gtcttatgga
     9901 gcaaaacccg cagtcacagc tgaaacttct tgtcacccgt ggtaaggagc aaggctatct
     9961 gacctatgcc gaggtcaatg accatctgcc ggaagatatc gtcgattcag atcagatcga
    10021 agacatcatc caaatgatca acgacatggg cattcaggtg atggaagaag caccggatgc
    10081 cgatgatctg atgctggctg aaaacaccgc ggacgaagat gctgccgaag ccgccgcgca
    10141 ggtgctttcc agcgtggaat ctgaaatcgg gcgcacgact gacccggtac gcatgtacat
    10201 gcgtgaaatg ggcaccgttg aactgttgac ccgcgaaggc gaaattgaca tcgctaagcg
    10261 tattgaagac gggatcaacc aggttcaatg ctccgttgct gaatatccgg aagcgatcac
    10321 ctatctgctg gaacagtacg atcgtgttga agcagaagaa gcgcgtctgt ccgatctgat
    10381 caccggcttt gttgacccga acgcagaaga agatctggca cctaccgcca ctcacgtcgg
    10441 ttctgagctt tcccaggaag atctggacga tgacgaagat gaagacgaag aagatggtga
    10501 cgacgatacc gccgatgatg acaacagcat cgacccggaa ctggctcgcg aaaaatttgc
    10561 ggaactgcgc gctcagtaca ttgtaacgcg tgacaccatc aaagcgaaag gccgcagtca
    10621 cgctgccgct caggaagaga tcctgaaact gtctgaagta ttcaaacagt tccgcctggt
    10681 gccgaagcag tttgactacc tggtcaacag catgcgcgtc atgatggacc gcgttcgtac
    10741 gcaagaacgt ctgatcatga agctctgcgt tgagcagtgc aaaatgccga agaaaaactt
    10801 cattaccctg tttaccggca acgaaaccag cgatacctgg ttcaacgcgg caattgcgat
    10861 gaacaagccg tggtcggaaa aactgcacga tgtctctgaa gaagtgcatc gcgccctgca
    10921 gaaactgcag cagattgaag aagaaaccgg cctgaccatc gagcaggtta aagatatcaa
    10981 ccgtcgtatg tccatcggtg aagcgaaagc ccgccgtgcg aagaaagaga tggttgaagc
    11041 gaacttgcgt ctggttattt ctatcgctaa gaaatacacc aaccgtggct tgcagttcct
    11101 tgacctgatt caggaaggta acatcggtct gatgaaagcg gttgataaat tcgaataccg
    11161 ccgtggttac aagttctcca cctacgcaac ctggtggatc cgtcaggcga tcacccgttc
    11221 tatcgcggat caggcgcgca ccatccgtat tccggtgcat atgattgaga ctatcaacaa
    11281 gctcaaccgt atttctcgcc agatgctgca agagatggga cgtgaaccaa cgccggaaga
    11341 actggctgaa cgcatgctga tgccggaaga caagatccgc aaagtgctga agatcgccaa
    11401 agagccaatc tccatggaaa cgccgatcgg tgatgatgaa gattcgcatc tgggggattt
    11461 catcgaggat accaccctcg agctgccgct ggattctgcg accaccgaaa gcctgcgtgc
    11521 ggcaacgcac gacgtgctgg ctggcctgac cgcgcgtgaa gcgaaagttc tgcgtatgcg
    11581 tttcggtatc gatatgaaca ccgaccacac gctggaagaa gtgggtaaac agttcgacgt
    11641 tacccgcgaa cgtatccgtc agatcgaagc gaaggcgctg cgcaaactgc gtcacccgag
    11701 ccgttctgaa gtgctgcgta gcttcctgga cgattaatcg gtaggccgga tcaggtgtta
    11761 cgccgcaccc ggcactagtc cctccgcaca aacgccacct tttcggtggc gtttttatcg
    11821 cccacgcacc accagcgcct ggtccagctc gcgatacgct tcaaccagtt tctccagtga
    11881 aacgcgactt aaaccgctgg gatttggcag gacccaaatc tgcgtcgaac caatggtgag
    11941 agtttgtttc ccccactgtg caccgcgctg gctgaatccc tgttcatatg cttgtttgcc
    12001 cagaatcgcc aacgcctgcg gctgataatc ttctattttt tcaattagct tacgcccgcc
    12061 tgcgtgcagc tcctgctttg acacttcatt ggcttgcacc gttggacggt ctaccagttt
    12121 ggtgacgcca caacgataat ccagcagatg ctgtgcctcc tgcggcttca actgacggtc
    12181 ggtaaacccg gcctgatata tcaccttcca gaagcggttt gccggatgag caaagggaaa
    12241 acccgttccg gcggatgaaa gaccagggtt gataccgcaa aacacgaccc gtaaccctgg
    12301 agccaaaata tcctcaacca tatttactcc tttctaacat caccaggaaa agtataacgg
    12361 attgaaaata cattgtttat aaaaacagca ggcgcgctgt aatggctgga ttgcgacacg
    12421 gagttacttt ataatccgct accatggccc cttagctcag tggttagagc aggcgactca
    12481 taatcgcttg gtcgctggtt caagtccagc aggggccacc agacatagca aaggctgacg
    12541 agaaatcgtc agcctttttc tttttatata tcagttactt tgcgtgccag taagccgccg
    12601 cgcgtacccg ctgtgggtca tactgttccg cttcaaagcg acggcttaaa ttcttaacga
    12661 ctttgccttc gccggttatc cagatgaagt aatcatcggc agggatttgc atctgcgcca
    12721 gacgcgcatc taccgcctgc tcatcatgtg ccagccattc gatattaaaa ccatcaaggt
    12781 gcgcgagata atcctgacag gcgttatccc gcacgctaac cagcgcacta acttgcggtt
    12841 taacggcaag tttgctcaac gtttccaggc ggtggcgcag tgcgggcatt ccggattcat
    12901 cgcagacata cagctgatac gcgtaatcct ctggcaccac cagcgaaccg cgcggacctg
    12961 ccaccgtaag tttatcgccc ggtttcgcct gcatcgccca gccgctggcg accccaccgt
    13021 cgtgaataaa gaaatcgatc gccagttcat ggcgtagttc gtcatacagc ggcgtatagt
    13081 cacgcgacgg tgggcgtggt ccttccggcc agacgatgcc ctcttccgtt accgttggcg
    13141 gcacaaagtg agtgcctggt tgaggaaaga agagcttgct gtgatcgtca aagccgcgcg
    13201 atgtaaaacc gtccagcgcc tcgccgccaa ggacaatgcg ctgaaaaccg gcgctgatgc
    13261 gctcaacgcg tagcacagtc agttcacgga agcgcagatc attgcgaacg cgctgtgggt
    13321 agcggggggt gttattcatt gttatcgcct tcgtgatggt aatcagatat atctaaataa
    13381 attccacaaa tgataatgat tgttaatcat gataaatgca agtgatttgt agcactgata
    13441 tatctgtagt ctgataagac gcgcaacctc ttatcaagca tcattgctca atgccggatg
    13501 cggcgtgaac gccttatccg gcctacatgt tgagtaaacc tgcgttta
//
LOCUS       DADSYM010000134        13207 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2415
            SAMN08039579-rid11723173.denovo.135, whole genome shotgun sequence.
ACCESSION   DADSYM010000134 DADSYM010000000
VERSION     DADSYM010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039579
            Sequence Read Archive: SRR6362533
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13207)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13207)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980162.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 03:39:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,484
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 4,347
            CDSs (with protein)               :: 4,347
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 43 of 85
            Pseudo Genes (incomplete)         :: 37 of 85
            Pseudo Genes (internal stop)      :: 19 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13207
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039579-rid11723173.denovo.135"
                     /strain="LGE2415"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8302 N 95.9628 W"
                     /collection_date="2015-08-24"
     gene            134..1633
                     /gene="pitB"
                     /locus_tag="J1Y83_003455"
     CDS             134..1633
                     /gene="pitB"
                     /locus_tag="J1Y83_003455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter PitB"
                     /protein_id="HBA7631570.1"
                     /translation="MLNLFVGLDIYTGLLLLLALAFVLFYEAINGFHDTANAVATVIY
                     TRAMQPQLAVVMAAFFNFFGVLLGGLSVAYAIVHMLPTDLLLNMGSTHGLAMVFSMLL
                     AAIIWNLGTWFFGLPASSSHTLIGAIIGIGLTNALLTGSSVMDALNLREVTKIFSSLI
                     VSPIVGLVIAGGLIFLLRRYWSGTKKRDRIHRIPEDRKKKKGKRKPPFWTRIALIVSA
                     AGVAFSHGANDGQKGIGLVMLVLVGIAPAGFVVNMNASGYEITRTRDAVTNFEHYLQQ
                     HPELPQKLVTMEPPLPATSTDGTQVTEFHCHPANTFDAIARVKTMLPGNMESYEPLSV
                     SQRSQLRRIMLCISDTSAKLAKLPGVSKEDQNLLKKLRSDMLSTIEYAPVWIIMAVAL
                     ALGIGTMIGWRRVAMTIGEKIGKRGMTYAQGMAAQMTAAVSIGLASYIGMPVSTTHVL
                     SSAVAGTMVVDGGGLQRKTVTSILMAWVFTLPAAIFLSGGLYWMALQLI"
     gene            complement(1682..2374)
                     /locus_tag="J1Y83_003456"
     CDS             complement(1682..2374)
                     /locus_tag="J1Y83_003456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate kinase"
                     /protein_id="HBA7631571.1"
                     /translation="MQSIIPPLIAVIGSDGSGKSTVCEHLITVVEKYGAAERVHLGKQ
                     AGNVGRAVTKLPLMGKSLHKTIERNQVKTAKKLPGPVPALVITAFVARRLLRFRHMLA
                     CRRRGLIVLTDRYPQDQIPGAYDGTVFPPNVEGGRFVSWLASQERKAFHWMASHKPDL
                     VIKLNVDLEVACARKPDHKRESLARKIAITPQLTFGGAQLVDIDANQPLEKVLVDAEK
                     AITDFMTARGYH"
     gene            2549..3262
                     /locus_tag="J1Y83_003457"
     CDS             2549..3262
                     /locus_tag="J1Y83_003457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate kinase"
                     /protein_id="HBA7631572.1"
                     /translation="MNQRNMSIINSTPVRVIAIVGCDGSGKSTLTASLVNELASRMPT
                     EHIYLGQSSGRIGEWISQLPVIGAPFGRYLRSKAAHVHEKPSTPPGNITALVIYLLSC
                     WRAYKFRKMLCKNQQGYLLITDRYPQVEVPGFRFDGPQLAKTTGGNGWIKMLRQRELK
                     LYQWMASYLPVLLIRLGIDEQTAFARKPDHQLAALQEKIAVTPQLTFNGARILELDGR
                     QPADEILQASLRAIHAALS"
     gene            3294..4052
                     /locus_tag="J1Y83_003458"
     CDS             3294..4052
                     /locus_tag="J1Y83_003458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA7631573.1"
                     /translation="MDALQTQTVKSTTAPQPNYIPGLIAVVGCDGTGKSTLTNDLVKS
                     LQQHWQTERRYLGLLSGEDGNKIKRLPLVGVWLERRLAAKSSKTQSMKTKSPALWAAV
                     IMYCFSLRRMANLRKVQRLAQSGVLVVSDRFPQAEISGFYYDGPGIGVERATGKISMF
                     LAQRERRLYQKMAQYRPELIIRLGIDIDTAISRKPDHDYAELQDKIGVMSTIGYNGTK
                     ILEIDSRAPYSEVLEQAQKAVSLVAIVSDRRSLT"
     gene            4098..5447
                     /locus_tag="J1Y83_003459"
     CDS             4098..5447
                     /locus_tag="J1Y83_003459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HBA7631574.1"
                     /translation="MAGFNIKHWFADGAFRTIIRNSAWLGSSNVVSALLGLLALSCAG
                     KGMTPAMFGVLVIVQSYAKSISDFIKFQTWQLVVQYGTPALTNNNPQQFRNVVSFSFS
                     LDIVSGAVAIVGGIALLPFLSHSLGLDDQSFWLAALYCTLIPSMASSTPTGILRAVDR
                     FDLIAVQQATKPFLRAAGSVVAWYFDFGFAGFVIAWYVSNLVGGTMYWWFAARELRRR
                     NIHNAFKLNLFESARHIKGAWSFVWSTNIAHSIWSARNSCSTVLVGIVLGPAAAGLFK
                     IAMTFFDAAGTPAGLLGKSFYPEVMRLDPRTIRPWLLGVKSGLLAGGIGILVALAVLI
                     VGKPLISLVFGVKYLEAYDLIQVMLGAIVISMLGFPQESLLLMAGKQRAFLVAQTIAS
                     IGYIVLLFMFCHLFGVLGAAFAYFGGQCLDVVLSLIPTLKAFFQRHSLLYNSTGEKS"
     gene            5447..6283
                     /locus_tag="J1Y83_003460"
     CDS             5447..6283
                     /locus_tag="J1Y83_003460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000984967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA7631575.1"
                     /translation="MMRKYFPLEASERLFVAIEEDDVVDAQVSLPPTIALSCTTEIIH
                     DNYALCLKFWLDGVNRQELLRLIRKQAKGDELTTDERKQFKYMRARYKHLRFAQRLYL
                     KKHQAGFLFGKTTVFLGHFQDGFRNGKKNIVSFYGNLLRVYLSSPVWWLVNYSLRHGQ
                     LETVNGFIAYRQKQMYILKEIIAKPQLTGREFHDVRKIISQQVSYYDTLRSLDPENKE
                     ALLISRFLAAINGLMGDKHDDMVADDMENRRSYDAPVALDSDIRQRLELLISRFPLYP
                     EQ"
     gene            6283..6843
                     /locus_tag="J1Y83_003461"
     CDS             6283..6843
                     /locus_tag="J1Y83_003461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtfJ family protein"
                     /protein_id="HBA7631576.1"
                     /translation="MPSRWIFALIIALLVPGAAAHNFVTGKTVTPVYIQEGGELLLNS
                     DDEIHYQKWNSTQLTGKVRIIQYIAGRKSAKKKNSLLIKAVEAANFSQDRFQPTTIVN
                     TDDAIFGTGYFVVGKIEKNKRRYPWAQFVIDGNGLGRVAWQLPEQSSTILVLNKAGRI
                     QWAKDGSLTPEEVDHVIALAQKLINE"
     gene            6874..7944
                     /gene="lptF"
                     /locus_tag="J1Y83_003462"
     CDS             6874..7944
                     /gene="lptF"
                     /locus_tag="J1Y83_003462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HBA7631577.1"
                     /translation="MKLVEHYIMRGTRRLVLIIVGFLIFIFASYSAQRYLTEAANGTL
                     ALDVVLDIVFYKVLIALEMLLPVGLYASVGVMLGQMYTDSEITAISAAGGSPGRLYKA
                     VLYLAIPLSIFVTLLSMYGRPWAYAQIYQLEQQSQSELDVRQLRAKKFNTNDNGRMIL
                     SQTVDQDNNRLTDALIYTSTANRTRIFRARSVDVVDPSPEKPTVMLHNGTAYLLDHQG
                     RDDNEQIYRNLQLHLNPLDQSPNVKRKAKSVTELARSVFPADHAELQWRQSRGLTALL
                     MALLAISLSRVKPRQGRFSTLLPLTLLFVAIFYGGDVCRTLVANGAIPLIPGLWLVPG
                     LMLMGLLMLVARDFSLLQKFSR"
     gene            7941..9020
                     /gene="lptG"
                     /locus_tag="J1Y83_003463"
     CDS             7941..9020
                     /gene="lptG"
                     /locus_tag="J1Y83_003463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HBA7631578.1"
                     /translation="MNVFSRYLIRHLFLGFAAAAGLLLPLFTTFNLINELDDVSPGGY
                     RWTQAVLVVLMTLPRTLVELSPFIALLGGIVGLGQLSKNSELTAIRSTGFSIFRIALV
                     ALVAGILWTVSLGAIDEWGASPLQQQALQIKSTATALGEDDDITGNMLWARRGNEFVT
                     VKSLNEQGQPVGVEIFHYRDDLSLESYIYARSATIEDDKTWILHGVNHKKWLNGKETL
                     EKSDYLAWQSTFTSMNLEELSMPGNTFSVRQLNHYIHYLQETGQPSSEYRLALWEKLG
                     QSILTLAMILLAVPFTFSAPRSPGMGSRLAVGVIVGLLTWISYQIMVNLGLLFALSAP
                     VTALGLPVAFVLVALSLVYWHDRQH"
     gene            complement(9055..10227)
                     /locus_tag="J1Y83_003464"
     CDS             complement(9055..10227)
                     /locus_tag="J1Y83_003464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HBA7631579.1"
                     /translation="MGLYDKYARLAGERLQFSDNGLTPFGTCIDEVYSATEGRIGNKK
                     VILAGTNNYLGLTFNHDAIREGQAALAAQGTGTTGSRMANGSYAPHLALEKEIAEFFN
                     RPTAIVFSTGYTANLGVISALADHNAVVLLDADSHASIYDACSLGGAEIIRFRHNDVN
                     DLERRMVRLGERAKEAIIIVEGIYSMLGDVAPLAEIVDIKHRLGGYLIVDEAHSFGVL
                     GATGRGLAEAVGVEDDVDIIVGTFSKSLASIGGFAVGSEAMEVLRYGSRPYIFTASPS
                     PSCIATVRSSLRTIASQPELRQKLMDNANHLYDGLQKLGYELSSHISPVVPVIIGSKE
                     EGLRIWRKLISLGVYVNLILPPAAPAGITLLRCSVNAAHSREQIDAIIQAFATLKQ"
     gene            complement(10227..10475)
                     /locus_tag="J1Y83_003465"
     CDS             complement(10227..10475)
                     /locus_tag="J1Y83_003465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HBA7631580.1"
                     /translation="MVNREIVMDYILSCLQDLVENGVEIKPDSDLVNDLGLESIKVMD
                     LLMMLEDRFDISIPINILLDVKTPAQLMETLLPWLENK"
     gene            complement(10507..11421)
                     /locus_tag="J1Y83_003466"
     CDS             complement(10507..11421)
                     /locus_tag="J1Y83_003466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001481799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HBA7631581.1"
                     /translation="MNQTVAVTGATGFIGKYIIDNLLARGFHVRALTRTARAHVNDNL
                     TWVRGSLEDTHSLSELVAGASAVVHCAGQVRGHKEEIFTRCNVDGSLRLMQAAKESGF
                     CQRFLFISSLAARHPELSWYANSKHVAEQRLTAMADEITLGVFRPTAVYGPGDKELKP
                     LFDWMLRGLLPRLGTPETLLSFLHVTDFAQAVGQWLSAETVQTQTYELCDGVAGGYDW
                     QRVQQLVADVRCGSVRMVGIPQPLLTCLADISTALSRLAGKEPMLTRSKIRELTHADW
                     SASNNRISEDINWFPGISLEHALRNGLF"
     gene            complement(11418..13151)
                     /locus_tag="J1Y83_003467"
     CDS             complement(11418..13151)
                     /locus_tag="J1Y83_003467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000262917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acyl-AMP ligase"
                     /protein_id="HBA7631582.1"
                     /translation="MVYMSNKIFTHSLPMRYADFPTLVDALDYAALSSAGMNFYDRRC
                     QLEDQLEYQTLKARAEAGAKRLLSLNLKKGDRVALIAETNSGFVEAFFACQYAGLVAV
                     PLAIPMGVGQRDSWSAKLQGLLASCQPAAIITGDEWLPLVNAATHNNNPELHVLSHAW
                     FKALPEADVVLQRPVSNDIAYLQYTSGSTRFPRGVIITHHEVMANLRAISHDGIKLRP
                     GDRCVSWLPFYHDMGLVGFLLTPVATQLSVDYLRTQDFAMRPLQWLKLISKNRGTVSV
                     APPFGYELCQRRVNEKDLAELDLSCWRVAGIGAEPISAEQLHQFAECFRQVNFDDKTF
                     MPCYGLAENALAVSFSDEACGVVVNEVDRDILEYQGKAVAPGAETRAVSTFVNCGKAL
                     PEHGIEIRNEADIPVAERVVGHICISGPSLMSGYFGDQVSQDEIAATGWLDTGDLGYL
                     LDGYLYVTGRIKDLIIIRGRNIWPQDIEYIAEQEPEIHSGDAIAFVTAQEKIILQIQC
                     RISDEARRGQLIHALAARIQSEFGVTADIDLLPPHSIPRTSSGKPARAEAKKRYQKAY
                     AASLHVQESLA"
BASE COUNT         3177 a         3258 c         3424 g         3348 t
ORIGIN      
        1 tgaaggctta ttgtctgata cacaagaaat aacacgcttt ttatcgttaa aaaatgatat
       61 ttcactttgc ccacgccgtg aaagtcactg ataatgcctc cgttcgtaaa ttcaaaatgg
      121 cgtaatctaa tatatgctaa atttatttgt tggccttgat atatatacgg ggcttttgtt
      181 attacttgct ctggcatttg tgttgttcta tgaagcaatc aatggttttc atgacacggc
      241 gaatgcggtg gcaaccgtta tttatacccg tgccatgcaa ccacagcttg ctgtggtcat
      301 ggcggcattt tttaactttt ttggcgtatt gcttggcggg cttagcgttg cctatgccat
      361 cgtccatatg ttgccaaccg atttgttgct gaatatgggg tcaactcacg gcctggcgat
      421 ggtcttttcc atgctgctgg cggcgattat ctggaacctg ggaacgtggt tctttggttt
      481 accggcctcc agttcgcaca ccttgattgg tgcgattatc ggcatcggtt taaccaacgc
      541 gctgttaacc ggctcatcgg tgatggatgc gttaaacctg cgtgaagtga ccaagatttt
      601 ctcctcgctg attgtctccc caattgtcgg tctggtcatt gcgggggggc tgattttcct
      661 gctgcgtcgc tactggagcg ggacgaaaaa gcgtgaccgt attcaccgca ttccggaaga
      721 tcgcaaaaag aaaaaaggca aacgtaagcc gccattctgg acgcgtattg ccctcattgt
      781 ttccgctgcg ggcgtggcgt tttcgcacgg cgcgaacgac gggcagaaag gaattggtct
      841 ggtgatgctg gtgctggtgg ggattgctcc cgccgggttt gtcgtcaata tgaacgcgtc
      901 cggctatgaa attacccgta cccgcgatgc cgtcaccaac ttcgaacact acctgcaaca
      961 gcatcctgaa ctgccgcaga agttggttac gatggaacct ccattgcctg cgacatcgac
     1021 tgatggcaca caagtaacgg agtttcactg tcatccggca aatacctttg atgcgattgc
     1081 gcgcgttaaa acgatgctgc caggtaatat ggaaagttac gagccgttaa gcgtgagtca
     1141 gcgcagccag ctgcgccgca ttatgctgtg catctctgat acctccgcga agctggcgaa
     1201 actgccaggc gtcagtaaag aagaccagaa cctgctgaaa aaactgcgca gcgatatgtt
     1261 aagcaccatt gagtatgcac cggtatggat catcatggcg gtagcactgg cgctcggcat
     1321 cggcaccatg attggctggc gtcgtgtcgc gatgaccatc ggtgagaaaa ttggtaagcg
     1381 tggcatgacg tatgcgcaag gcatggcggc acaaatgacg gcggcagtgt ctatcggcct
     1441 tgccagttat attgggatgc ccgtctccac aacacacgta ctctcgtctg cagtagcagg
     1501 gacgatggtg gtggacggcg gtgggttaca gcgtaaaacg gtaaccagca tcctgatggc
     1561 gtgggtgttt actttaccgg cggcgatttt tctttctggt gggttgtact ggatggcatt
     1621 gcagttgatt taaccttcct gaaaatgccc ggtcctggcg atcgggcatt tccatttttc
     1681 actagtgata accacgcgcg gtcataaaat ccgtaatcgc tttttctgca tcaaccagca
     1741 ccttttccag tggctgattg gcgtcgatat caaccagttg tgcaccacca aaggttaact
     1801 gtggcgttat ggcaatcttc ctcgccagcg attcccgttt atggtcgggt ttacgcgcgc
     1861 aggcaacttc aaggtcaaca ttgagtttga tgaccagatc aggcttgtgg ctcgccatcc
     1921 agtgaaacgc tttacgttcc tgacttgcca gccatgagac aaaacgacca ccttcaacgt
     1981 taggcggaaa caccgtacca tcataagcgc caggaatctg gtcctgcgga taacggtcgg
     2041 ttaaaacaat taacccgcga cgacgacagg caagcatatg acgaaagcgc agtaagcggc
     2101 gggcgacaaa cgctgtaatt accagcgccg gaactggtcc aggcaatttt tttgctgttt
     2161 ttacctgatt tcgttcaatt gttttatgta aggattttcc catcaacggt aattttgtca
     2221 ctgcacgacc gacatttccg gcctgttttc ctaaatgaac cctttcggca gcaccatatt
     2281 tttcgacaac ggtaataaga tgttcacaca ccgttgactt gcctgaacca tcgctaccaa
     2341 taacggcaat taatggaggt attattgact gcatgaatat atccttaaat ttaaatatcc
     2401 atccaaaata tttatttggt taatatgttt ttgatgaaag cgtaattcag gtcaatgtca
     2461 caattaacca ttgtcacaat aaggttgaac ggatattctg agcacggacc acgtaatatt
     2521 caatacatta tttgctgccg ggtttttcgt gaatcaacgt aatatgtcaa taattaattc
     2581 cacgccagtg cgtgttattg ccattgtagg atgtgatggt tcaggtaaat cgaccctcac
     2641 ggcaagcctg gtaaatgaac tggcatcaag aatgccaaca gaacacattt atctcgggca
     2701 atcgtccggg cgaattggcg aatggatttc acagctacct gttattggcg caccttttgg
     2761 gcgttatttg cgaagtaaag cggcacatgt gcacgaaaag ccctcaacac cgcctggcaa
     2821 tattactgcg ctggttatct atctgctttc ctgctggcgg gcgtacaagt ttcgcaaaat
     2881 gttgtgtaaa aaccagcaag gctatctgct catcaccgac cgctacccgc aggttgaagt
     2941 gccgggtttc cgctttgatg gcccgcaatt ggcaaaaacc acgggcggta acggttggat
     3001 aaaaatgtta aggcagcgtg agctgaagct gtaccaatgg atggcgtctt atttgcctgt
     3061 attgttgatt cgtcttggca ttgatgaaca aaccgcgttt gcgcgtaaac ctgaccacca
     3121 actggcagcg ttacaggaaa aaatcgccgt tacgccgcaa ctgacattca atggcgccag
     3181 gatccttgaa ctggatggtc ggcaaccagc cgatgaaatt ctgcaagcgt cactacgcgc
     3241 aattcacgcc gcgctttcct gatcaccaaa atggataggg gatgattaaa cgaatggatg
     3301 cactacaaac acaaactgtt aagagtacaa ccgcgccgca gcccaactac attccggggc
     3361 tgattgcggt ggtcgggtgt gatggcaccg gtaaatccac actgacaaat gacctggtga
     3421 aatcgctgca acaacactgg caaaccgagc ggcgctattt ggggctgctc tccggcgaag
     3481 acggcaacaa aatcaaacga ttgccgttgg ttggtgtctg gctcgaacgg cgactggcgg
     3541 ccaaatcctc gaaaacccaa agcatgaaaa ccaaatctcc ggcgctatgg gcggcggtga
     3601 ttatgtactg cttctcgctg cgaagaatgg cgaatctgcg caaggttcag cgactggcgc
     3661 aaagtggcgt tctggtggtc agcgatcgct tcccgcaggc tgaaatttcg ggcttttatt
     3721 atgacggacc ggggattggt gtcgaacgtg cgaccgggaa aatcagcatg tttctggcgc
     3781 agcgtgaacg gcgtttatac caaaaaatgg cgcaataccg tcctgaatta atcattcgct
     3841 tggggattga tattgatact gccatctccc gcaagcctga ccatgactat gccgagctgc
     3901 aggacaaaat cggtgttatg tcgacgattg gctataacgg cacaaaaatt cttgagattg
     3961 attccagagc gccttacagc gaggtgctgg aacaggcaca gaaggcggtt tctctggttg
     4021 ccatcgtttc tgaccgccga agtttaactt aaggctggcg ggatactttt tactatcaac
     4081 gataaggtca ggctgaattg gcgggtttta acatcaaaca ttggtttgca gatggcgcgt
     4141 ttcgcaccat tattcgcaat agcgcctggt taggctccag taatgttgtg agcgctttgc
     4201 tggggctgtt ggcgctctcg tgtgccggta aagggatgac gcccgccatg tttggcgtac
     4261 tggtaattgt gcaatcgtac gccaaatcga tcagcgattt tattaagttt cagacatggc
     4321 aactggtggt tcagtacgga acgccggcct tgaccaacaa taatccgcag caatttcgca
     4381 atgttgtctc attttccttc tcgctggata tcgtcagcgg cgcggtggcg attgtcggcg
     4441 gcattgcctt actgccattc ctttcccatt cattaggtct ggatgaccaa agcttttggc
     4501 tggcggcgct ctattgcacg ctcattcctt caatggcttc ctccacgccg accggcattc
     4561 tgcgtgcggt agatcgcttt gatttgattg ctgtacagca ggcgacgaaa ccttttctgc
     4621 gcgcagcggg gagcgtcgta gcctggtatt ttgactttgg ttttgcgggt tttgttattg
     4681 cctggtacgt gtcgaatctg gttggcggca ccatgtactg gtggtttgcc gcgcgcgaat
     4741 tacgccgcag aaatatccat aacgccttca aattgaatct gtttgagtct gcccgacaca
     4801 ttaaaggcgc gtggagtttt gtctggtcaa ccaacattgc ccactccatc tggtcggcgc
     4861 gtaactcgtg cagcacagtg ttggtgggga tcgtgttagg acccgctgct gccgggttat
     4921 ttaaaatcgc catgacattc ttcgatgccg ccggaacgcc agcgggttta ctgggcaaaa
     4981 gtttttatcc agaggtgatg cgtttagatc cacgcaccat cagaccgtgg ttgttaggcg
     5041 tgaagtccgg tttactggcg ggcggaatcg gtattctggt ggcgctcgcg gtgttgatcg
     5101 tcggcaagcc gctcatttcg ctggtgtttg gcgttaagta tctcgaagcg tatgacctga
     5161 ttcaggtgat gctgggcgca attgtgattt cgatgctggg cttcccacag gagtcattgc
     5221 tattgatggc gggaaaacag cgcgcttttc tcgtggcgca aaccatcgcc tcaatcggct
     5281 acatcgtatt gctgttcatg ttctgtcatc tgtttggcgt gctgggggct gcatttgctt
     5341 acttcggtgg gcaatgtctg gatgtggtgc tctcgctaat tccgacctta aaggcatttt
     5401 ttcagcgcca ttccttgctt tataactcaa ccggagagaa atcctgatga tgagaaaata
     5461 tttcccgctt gaggcgagcg agcgtctatt tgttgcgatt gaagaagatg atgttgttga
     5521 tgcacaggtc agtttgccgc cgactatcgc actaagttgt accaccgaaa tcatccatga
     5581 taattacgcg ttatgcctga aattctggct ggatggtgtt aatcgtcagg agttactgcg
     5641 tttaatccgc aaacaggcga aaggcgatga gctgacgaca gacgagcgca aacagtttaa
     5701 atacatgcga gcgcgctaca agcatctgcg ttttgcgcaa cggctgtatt taaagaagca
     5761 tcaggccgga tttttattcg gcaaaacgac cgtttttctt gggcattttc aggatggctt
     5821 tcgtaacggt aagaaaaata ttgtgtcgtt ttacggtaac ctgttacgtg tttatttgag
     5881 ctcccctgtc tggtggctgg tgaactattc attgcgccat ggccagttag aaaccgtgaa
     5941 cggttttatc gcctaccgcc agaagcaaat gtacatcctg aaagagatta ttgctaaacc
     6001 gcaactaact ggcagggaat ttcatgatgt gcgaaaaatc atcagccagc aggtttctta
     6061 ttacgacacc ttaagatcgc ttgatcctga aaataaagag gcgttactga tttcccgttt
     6121 tctcgccgcc attaatggtc tgatggggga taagcacgac gacatggtgg cggatgatat
     6181 ggagaaccgc cggtcatatg atgcaccggt ggcgctggat agcgatattc gccagcgttt
     6241 agaactgctt atttcgcgtt tcccactata tccggagcaa taatgccatc tcgttggatt
     6301 ttcgctctga tcatcgcact cttagtgcca ggcgcggcgg cacacaattt cgtcactggc
     6361 aaaacggtta ctcctgttta tatccaggaa ggcggcgagc tgcttctgaa tagcgacgac
     6421 gaaattcact accaaaagtg gaacagcaca caattgacgg gaaaagtccg cattattcag
     6481 tatatcgccg gacgtaagtc tgcgaagaaa aagaactcac tgttaattaa agcggttgaa
     6541 gcggcaaact tttcgcagga tcgttttcag cccaccacta ttgtgaacac cgatgatgcc
     6601 atctttggta ctggctattt tgtcgttggc aaaatcgaaa aaaataaacg ccgctatccg
     6661 tgggcgcaat tcgttattga cggcaatggc ctgggacgcg ttgcctggca attgccggag
     6721 cagagttcaa ccattctggt attgaataaa gccgggcgaa ttcagtgggc aaaggacggc
     6781 agcctgacac cggaagaggt agatcatgtc attgcgctgg cgcagaaact gatcaatgaa
     6841 taaaaaattc aaaggattga gggcgttagc gagatgaagc tggttgaaca ctacatcatg
     6901 cgtggtacgc gccgtctggt gctgattatc gtcggttttc tgatctttat tttcgccagc
     6961 tactccgcac agcgttatct gaccgaagcg gcaaatggga cattagctct tgatgttgtg
     7021 ctggatatcg ttttctacaa agtgctgatt gcactggaga tgctgttacc tgttggactg
     7081 tatgcgtcag ttggcgtgat gctagggcaa atgtataccg actcggaaat taccgctatc
     7141 tccgcggcgg ggggcagtcc gggacgtttg tacaaagccg tcctttatct ggcgataccg
     7201 ctaagtattt ttgtcaccct tctgtcgatg tatggtcgtc cctgggctta tgcgcagatt
     7261 tatcaactgg agcaacaatc gcagtcggag ctggatgttc gtcagttgcg ggcaaagaaa
     7321 tttaacacta acgataacgg acgaatgatc ctttcgcaga cggttgatca ggataataat
     7381 cgcctgactg acgcgctgat ttatacttct actgccaatc gaacccgcat tttccgcgcc
     7441 cgttcggttg atgtggttga cccatcaccc gagaaaccga ccgttatgtt gcataacggt
     7501 accgcctatc ttctcgatca tcaggggcgt gacgacaacg aacagatcta ccgtaatctg
     7561 caattgcatc tgaatccgct ggatcaaagc cctaacgtca aacgcaaagc aaaatcggtc
     7621 acggagctgg cgcgctccgt ctttccagcc gatcatgccg aactgcaatg gcgacaaagc
     7681 cgtggcctga ccgcattgtt gatggcgctg ctggccattt cattaagtcg ggtaaaaccg
     7741 cggcaggggc gattttcaac gctactgcca ctgacgttgc tgtttgttgc cattttttat
     7801 ggcggcgacg tctgccgtac gctggtggct aacggcgcga ttcccctcat tcctggtttg
     7861 tggttagtac ccggactcat gctaatgggc ctgctgatgc tggtcgcacg cgacttctct
     7921 ttgctgcaga aattttcccg atgaatgttt tcagtcgcta tttaatccgt cacctctttc
     7981 tcggttttgc cgccgccgca gggctattac tgccgctttt taccaccttc aacctgatta
     8041 acgaactgga tgatgtcagc ccgggcggtt atcgctggac ccaggcggtg ctggtggtgc
     8101 tgatgacctt accgcgcaca ctggtcgaac tttcgccgtt tatcgcctta ttgggaggta
     8161 ttgtcggtct cgggcagcta tcgaaaaaca gtgagcttac cgccattcgc agcacggggt
     8221 tttctatctt ccgtattgca ctggtggcgc tggttgccgg gatactgtgg actgtttcgt
     8281 taggcgcgat agatgagtgg ggggcgtcgc cattgcagca acaggcgctg caaatcaaat
     8341 cgaccgccac cgcgttgggg gaggacgatg acattaccgg caatatgctt tgggccaggc
     8401 gcggtaatga atttgtgacg gtgaaatcgc tgaacgagca gggccagccg gtgggcgtgg
     8461 agatttttca ttaccgcgat gatctctcgc tcgaatccta catttatgcg cgcagtgcca
     8521 ccattgaaga tgacaaaacg tggatcctgc atggtgtgaa tcataaaaaa tggctgaatg
     8581 gcaaagaaac gctggaaaaa tctgattatc ttgcctggca atcgaccttc accagtatga
     8641 atcttgaaga gttatcgatg ccggggaata ctttttctgt ccgtcagctt aatcattaca
     8701 tccattattt gcaggaaacc gggcaaccca gcagcgaata ccgccttgca ctgtgggaaa
     8761 aactggggca atccatcttg actctggcga tgattttgct ggctgtgccg tttaccttta
     8821 gtgccccgcg ctcgccaggg atgggtagcc gtctcgctgt aggcgtcatc gttggtttac
     8881 tcacctggat cagctatcaa atcatggtca atctgggatt gctatttgcg ttgagcgcac
     8941 ctgttaccgc gctcggttta ccggtagcgt ttgtgctggt ggcgttgagc ctggtgtatt
     9001 ggcatgacag acaacattaa acccgccgtg gcgcaacacc acggcgggtt cacattactg
     9061 cttcagagta gcaaatgcct gaatgatggc atcgatttgc tcacgactgt gggcagcgtt
     9121 tacgctacag cgcagcagcg tgatgccagc aggcgcggca ggtggcagga taagattcac
     9181 ataaacgccc agcgaaataa gcttgcgcca gatgcgtaaa ccttcctctt tcgaaccgat
     9241 tattactggc actaccgggc taatatgtga acttaactcg taacccagtt tttgcagacc
     9301 atcatacaga tggttggcgt tatccatcag cttttggcgc agctctggtt gtgaggcaat
     9361 ggttcttaac gacgagcgca cggtcgcaat gcaggacgga gaaggagagg cggtaaaaat
     9421 atacgggcgg ctgccgtagc gcagcacttc catcgcttcc gaaccgacag caaaaccgcc
     9481 aatcgaggcc agacttttac tgaaggtgcc aacgataata tccacatcat cttcaacgcc
     9541 gacagcttcg gccagtccgc gccccgtagc gcctaacacg ccaaacgaat gtgcttcatc
     9601 gacaatcaaa taaccaccga gacgatgttt gatatcaaca atttctgcca gcggcgcaac
     9661 gtcaccgagc atactgtaga tgccttcaac gataataatc gcctctttcg cccgttcacc
     9721 gaggcggacc atgcgccgtt ccagatcgtt gacgtcatta tggcggaaac gaataatctc
     9781 cgcgccgccg agagaacaag cgtcataaat actggcgtgg ctgtcggcat ccagtaacac
     9841 cacggcgtta tggtcggcaa gtgcgctgat gacacctaaa ttagcggtat aaccggttga
     9901 aaaaacgata gcggtagggc gattgaaaaa ctcggcaatc tctttctcta acgccagatg
     9961 cggtgcataa ctgccgtttg ccatacgaga accggtggtg ccggtgcctt gggccgctaa
    10021 cgctgcctga ccttcgcgga tagcatcatg attaaaggtt aaaccgagat aattattggt
    10081 cccggccaga atcacttttt tattgccgat ccggccttcg gtggcggaat acacttcatc
    10141 aatacaggtg ccaaatggtg ttaacccgtt gtcactaaat tgcaggcgct cgccagcaag
    10201 gcgcgcatat ttatcgtata gccccattat ttattctcca gccatggaag tagagtttcc
    10261 attaattgcg caggggtttt gacatccagc agaatattaa taggaatgga aatatcgaat
    10321 ctatcttcca gcatcattaa gagatccatc actttgatcg actccaggcc aagatcgttg
    10381 acgagatcgc tgtcgggctt aatttccaca ccgttttcga ccaaatcctg taaacaggaa
    10441 aggatgtaat ccattactat ttcacgattt accatagaaa cgttaacaca cctttaaaca
    10501 agacaatcaa aatagcccgt tgcgtaatgc gtgttccagg ctaatcccgg gaaaccaatt
    10561 aatatcttca gaaatacggt tattacttgc cgaccagtcg gcatgggtta attcacggat
    10621 tttcgaacga gtcagcatcg gttctttacc cgccagacgg ctcaacgcgg tactgatatc
    10681 cgcaaggcag gtgagtagcg gctgaggaat accgaccatc cgcacggaac cacagcggac
    10741 atcggcaaca agctgttgga cacgttgcca gtcatagcct ccggcgacgc catcgcataa
    10801 ttcataggtt tgcgtctgta cagtttcggc gcttaaccac tgacccactg cttgcgcaaa
    10861 atcggtgacg tgaagaaaag agagcagtgt ctctggcgtg ccaagtcgtg gcagcagacc
    10921 gcgcagcatc cagtcaaaca gcggttttaa ctctttatcg ccgggaccat acacggctgt
    10981 cgggcgaaaa acgcccaacg taatttcgtc agccattgca gtcagccgtt gttcggcgac
    11041 gtgtttggaa tttgcgtacc aggagagttc gggatggcgc gccgccagcg aagagataaa
    11101 cagaaaacgt tggcaaaagc cgctctcttt tgccgcttgc atcaggcgca ggctgccgtc
    11161 aacgttgcag cgggtaaaaa tctcttcttt gtgcccgcgc acttgcccgg cgcaatggac
    11221 caccgcgctg gctccggcaa ccagttcgct aagcgaatgt gtatcttcca gcgaaccgcg
    11281 cacccaagtg agattatcat tgacgtgagc gcgggcagta cgcgtcaatg cgcgaacgtg
    11341 aaagccgcgg gcgagcaggt tatcaataat atatttaccg ataaacccgg tagcgcccgt
    11401 caccgcgaca gtttgattca tgccagggat tcctgcacat gaagactggc agcataagcc
    11461 ttctgataac gttttttcgc ttccgcacgg gcaggcttgc cggaggatgt tcggggaata
    11521 ctgtggggcg gcaacagatc gatatccgcg gtaacgccaa attcgctttg gatccgagct
    11581 gccagcgcgt gaataagctg cccgcgacgt gcttcgtcgc tgatccgaca ctggatctgc
    11641 aaaatgattt tttcctgggc ggtaacaaaa gcaatcgcat cgccagaatg aatttccggc
    11701 tcctgttccg caatgtattc aatatcctgc ggccagatat tacggccacg aataataatc
    11761 agatctttaa tgcgtccggt gacatacaga taaccgtcca gcagataacc aaggtcgcca
    11821 gtgtctaacc agcccgtcgc ggcaatctcg tcttgcgaaa cctggtcgcc aaagtacccg
    11881 ctcatcaggc tggggccgga gatgcaaata tggcctacca cacgttccgc gacaggtata
    11941 tctgcttcat tgcggatttc aatgccatgt tccggcaacg ctttaccgca gttgacgaaa
    12001 gtcgatacgg cgcgtgtttc agcacccggc gcgacggctt taccctggta ttcgaggatg
    12061 tcgcgatcca cttcgttaac cacaaccccg caggcttcat cagagaagct gacagccagc
    12121 gcattttctg ccagtccgta gcacggcatg aaagttttat cgtcaaagtt aacctgacgg
    12181 aaacattcag cgaattgatg gagttgttct gcggatatgg gttctgcgcc aataccagcg
    12241 acgcgccagc aggaaagatc cagttcagcg agatcttttt cattcacgcg gcgctggcac
    12301 aattcatagc caaacggcgg tgcaacggaa acggtgccac gatttttact gatcaattta
    12361 agccattgca gaggacgcat ggcaaaatcc tgagtgcgca aataatctac tgaaagctgc
    12421 gtggcgacgg gggtcaggag aaagccgacc agtcccatat catggtagaa aggcagccag
    12481 gagacgcagc ggtcgccagg gcgtaattta atcccatcgt ggcttatagc acgtagatta
    12541 gccatcactt cgtgatgggt gataatgacg ccacggggaa aacgggtgct gccggaggtg
    12601 tactggaggt aggcgatatc gtttgaaact ggacgctgga gcacaacatc ggcttccggt
    12661 aatgccttaa accaggcgtg gcttaaaaca tgtaattcgg ggttgttgtt atgcgtcgcg
    12721 gcattgacca gtggcaacca ctcatcacca gtgataatgg ctgcgggctg gcaacttgcc
    12781 agtaaaccct gcagtttggc gctccaggaa tcccgctgac ctacgcccat tggaatcgcc
    12841 aacgggacgg cgactaagcc ggcatactgg caggcaaaaa aagcctctac aaacccgcta
    12901 ttcgtttcgg caatcagcgc cacgcgatcg ccttttttca ggttcagcga taacaacctt
    12961 ttcgcaccag cttcggcacg cgcttttaac gtctgatatt ccagttgatc ttcaagttgg
    13021 caacgtctgt cataaaaatt cattccggcg ctactcagag cggcgtagtc caaagcatca
    13081 accagcgttg gaaaatcggc atagcgcata ggtagggaat gcgtaaagat tttattagac
    13141 atatacacca cgaaaaaatt ggatatcaaa aataaaatga caataatgcg gtggattata
    13201 taggcat
//
LOCUS       DADSYM010000135        12776 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2415
            SAMN08039579-rid11723173.denovo.136, whole genome shotgun sequence.
ACCESSION   DADSYM010000135 DADSYM010000000
VERSION     DADSYM010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039579
            Sequence Read Archive: SRR6362533
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12776)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12776)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980162.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 03:39:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,484
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 4,347
            CDSs (with protein)               :: 4,347
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 43 of 85
            Pseudo Genes (incomplete)         :: 37 of 85
            Pseudo Genes (internal stop)      :: 19 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12776
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039579-rid11723173.denovo.136"
                     /strain="LGE2415"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8302 N 95.9628 W"
                     /collection_date="2015-08-24"
     gene            173..1687
                     /locus_tag="J1Y83_003468"
     CDS             173..1687
                     /locus_tag="J1Y83_003468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-carnitine/gamma-butyrobetaine antiporter"
                     /protein_id="HBA7631583.1"
                     /translation="MKNEKRKTGIEPKVFFPPLIIVGILCWLTVRDLDAANVVINAVF
                     SYVTNVWGWAFEWYMVVMLFGWFWLVFGPYAKKRLGNEPPEFSTASWIFMMFASCTSA
                     AVLFWGSIEIYYYISTPPFGLEPNSTGAKELGLAYSLFHWGPLPWATYSFLSVAFAYF
                     FFVRKMEVIRPSSTLVPLVGEKHAKGLFGTIVDNFYLVALIFAMGTSLGLATPLVTEC
                     MQWLFGIPHTLQLDAIIITCWIILNAICVACGLQKGVRIASDVRSYLSFLMLGWVFIV
                     SGASFIMNYFTDSVGMLLMYLPRMLFYTDPIAKGGFPQGWTVFYWAWWVIYAIQMSIF
                     LARISRGRTVRELCFGMVLGLTASTWILWTVLGSNTLLLIDKNIINIPNLIEQYGVAR
                     AIIETWAALPLSTATMWGFFILCFIATVTLVNACSYTLAMSTCREVRDGEEPPLLVRI
                     GWSVLVGIIGIVLLALGGLKPIQTAIIAGGCPLFFVNIMVTLSFIKDAKQNWKD"
     gene            1718..2860
                     /gene="caiA"
                     /locus_tag="J1Y83_003469"
     CDS             1718..2860
                     /gene="caiA"
                     /locus_tag="J1Y83_003469"
                     /EC_number="1.3.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetainyl-CoA dehydrogenase"
                     /protein_id="HBA7631584.1"
                     /translation="MDFNLNDEQELFVAGIRELMASENWEAYFAECDRDSVYPERFVK
                     ALADMGIDSLLIPEEHGGLDAGFVTLAAVWMELGRLGAPTYVLYQLPGGFNTFLREGT
                     QEQIDKIMAFRGTGKQMWNSAITEPGAGSDVGSLKTTYTRRNGKIYLNGSKCFITSSA
                     YTPYIVVMARDGASPDKPVYTEWFVDMSKPGIKVTKLEKLGLRMDSCCEITFDDVELD
                     EKDMFGREGNGFNRVKEEFDHERFLVALTNYGTAMCAFEDAARYANQRVQFGEAIGRF
                     QLIQEKFAHMAIKLNSMKNMLYEAAWKADNGTITSGDAAMCKYFCANAAFEVVDSAMQ
                     VLGGVGIAGNHRISRFWRDLRVDRVSGGSDEMQILTLGRAVLKQYR"
     gene            2989..4206
                     /gene="caiB"
                     /locus_tag="J1Y83_003470"
     CDS             2989..4206
                     /gene="caiB"
                     /locus_tag="J1Y83_003470"
                     /EC_number="2.8.3.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-carnitine CoA-transferase"
                     /protein_id="HBA7631585.1"
                     /translation="MDHLPMPKFGPLAGLRVVFSGIEIAGPFAGQMFAEWGAEVIWIE
                     NVAWADTIRVQPNYPQLSRRNLHALSLNIFKDEGREAFLKLMETTDIFIEASKGPAFA
                     RRGITDEVLWQHNPKLVIAHLSGFGQYGTEEYTNLPAYNTIAQAFSGYLIQNGDVDQP
                     MPAFPYTADYFSGLTATTAALAALHKVRETGKGESIDIAMYEVMLRMGQYFMMDYFNG
                     GEMCPRMTKGKDPYYAGCGLYKCADGYIVMELVGITQIAECFKDIGLAHLLGTPEIPE
                     GTQLIHRIECPYGPLVEEKLDAWLAAHTIAEVKERFAELNIACAKVLTVPELESNPQY
                     VARESITQWQTMDGRTCKGPNIMPKFKNNPGQIWRGMPSHGMDTAAILKNIGYSENDI
                     QELVSKGLAKVED"
     gene            4279..5832
                     /gene="caiC"
                     /locus_tag="J1Y83_003471"
     CDS             4279..5832
                     /gene="caiC"
                     /locus_tag="J1Y83_003471"
                     /EC_number="6.2.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetaine/carnitine-CoA ligase"
                     /protein_id="HBA7631586.1"
                     /translation="MDIIGGQHLRQMWDDLADVYGHKTALICESSSGVVNRYSYLELN
                     QEINRTANLFYVLGIRKGDKVALHLDNCPEFIFCWFGLAKIGAIMVPINARLLREEST
                     WILQNSQACLLVTSAQFYPMYQQIQQEDASQLRHICLTDMVLPADDGVSSFTQRKNQQ
                     PATLCYAPPLSTDDTAEILFTSGTTSRPKGVVITHYNLRFAGYYSAWQCALRDDDVYL
                     TVMPAFHIDCQCTAAMAAFSAGATFVLVEKYSARAFWGQVQKYHATITECIPMMIRTL
                     MVQPPSANDQQHRLREVMFYLNLSEQEKDAFCERFGVRLLTSYGMTETIVGIIGDRPG
                     DKRRWPSIGRAGFCYEAEIRDDHNRPLPAGEIGEICIKGVPGKTIFKEYFLNPKATAK
                     VLEADGWLHTGDTGYRDEEGFFYFVDRRCNMIKRGGENVSCVELENIIATHPKIQDIV
                     VVGIKDSIRDEAIKAFVVLNEGETLSEEEFFRFCEQNMAKFKVPSYLEIRKDLPRNCS
                     GKIIRKNLK"
     gene            5941..6726
                     /gene="caiD"
                     /locus_tag="J1Y83_003472"
     CDS             5941..6726
                     /gene="caiD"
                     /locus_tag="J1Y83_003472"
                     /EC_number="4.2.1.149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetainyl-CoA hydratase"
                     /protein_id="HBA7631587.1"
                     /translation="MSESLHLTRNGSILEITLDRPKANAIDAKTSFEMGEVFLNFRDD
                     PQLRVAIITGAGEKFFSAGWDLKAAAEGEAPDADFGPGGFAGLTEIFNLDKPVIAAVN
                     GYAFGGGFELALAADFIVCADNASFALPEAKLGIVPDSGGVLRLPKILPPAIVNEMVM
                     TGRRMGAEEALRWGVVNRVVSQAELMDNARELAQQLVNSAPLAIAALKEIYRTTSEMP
                     VEEAYRYIRSGVLKHYPSVLHSEDAIEGPLAFAEKRDPVWKGR"
     gene            6732..7322
                     /gene="caiE"
                     /locus_tag="J1Y83_003473"
     CDS             6732..7322
                     /gene="caiE"
                     /locus_tag="J1Y83_003473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308065.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine operon protein CaiE"
                     /protein_id="HBA7631588.1"
                     /translation="MSYYAFEGLIPVVHPTAFVHPSAVLIGDVIVGAGVYIGPLASLR
                     GDYGRLIVQAGANIQDGCIMHGYCDTDTIVGENGHIGHGAILHGCVIGRDALVGMNSV
                     IMDGAVIGEESIVAAMSFVKAGFRGEKRQLLMGTPARAVRSVSDDELHWKRLNTKEYQ
                     DLVGRCHAALHETQPLRQMEENRPRLQGTTDVTPKR"
     gene            complement(7441..7836)
                     /gene="caiF"
                     /locus_tag="J1Y83_003474"
     CDS             complement(7441..7836)
                     /gene="caiF"
                     /locus_tag="J1Y83_003474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414576.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine metabolism transcriptional regulator
                     CaiF"
                     /protein_id="HBA7631589.1"
                     /translation="MCEGCVEKPLYLLIAEWMMAENRWVIAREISIHFDIEHSKAVNT
                     LTYILSEVTEISCEVKMIPNKLEGRGCQCQRLVKVVDIDEQIYARLRNNSRDKLVGVR
                     KTPRIPAVPLTELNREQKWQMMLSKSMRR"
     gene            complement(8097..11318)
                     /gene="carB"
                     /locus_tag="J1Y83_003475"
     CDS             complement(8097..11318)
                     /gene="carB"
                     /locus_tag="J1Y83_003475"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HBA7631590.1"
                     /translation="MPKRTDIKSILILGAGPIVIGQACEFDYSGAQACKALREEGYRV
                     ILVNSNPATIMTDPEMADATYIEPIHWEVVRKIIEKERPDAVLPTMGGQTALNCALEL
                     ERQGVLEEFGVTMIGATADAIDKAEDRRRFDVAMKKIGLETARSGIAHSMEEALAVAA
                     EVGFPCIIRPSFTMGGSGGGIAYNREEFEEICARGLDLSPTKELLIDESLIGWKEYEM
                     EVVRDKNDNCIIVCSIENFDAMGIHTGDSITVAPAQTLTDKEYQIMRNASMAVLREIG
                     VETGGSNVQFAVNPKNGRLIVIEMNPRVSRSSALASKATGFPIAKVAAKLAVGYTLDE
                     LMNDITGGRTPASFEPSIDYVVTKIPRFNFEKFAGANDRLTTQMKSVGEVMAIGRTQQ
                     ESLQKALRGLEVGATGFDPKVSLDDPEALTKIRRELKDAGAERIWYIADAFRAGLSVD
                     GVFNLTNIDRWFLVQIEELVRLEEKVAEVGITGLHAEFLRQLKRKGFADARLAKLAGV
                     REAEIRKLRDQYDLHPVYKRVDTCAAEFATDTAYMYSTYEEECEANPSTDREKIMVLG
                     GGPNRIGQGIEFDYCCVHASLALREDGYETIMVNCNPETVSTDYDTSDRLYFEPVTLE
                     DVLEIVRIEKPKGVIVQYGGQTPLKLARALEAAGVPVIGTSPDAIDRAEDRERFQHAV
                     ERLKLKQPANATVTTIEMAVEKAKEIGYPLVVRPSYVLGGRAMEIVYDEADLRRYFQT
                     AVSVSNDAPVLLDHFLDDAVEVDVDAICDGEMVLIGGIMEHIEQAGVHSGDSACSLPA
                     YTLSQEIQDVMRQQVQKLAFELQVRGLMNVQFAVKNNEVYLIEVNPRAARTVPFVSKA
                     TGVPLAKVAARVMAGKSLAEQGVTKEVIPPYYSVKEVVLPFNKFPGVDPLLGPEMRST
                     GEVMGVGRTFAEAFAKAQLGSNSTMKKHGRALLSVREGDKERVVDLAAKLLKQGFELD
                     ATHGTAIVLGEAGINPRLVNKVHEGRPHIQDRIKNGEYTYIINTTSGRRAIEDSRVIR
                     RSALQYKVHYDTTLNGGFATAMALNADATEKVISVQEMHAQIK"
     gene            complement(11336..12484)
                     /gene="carA"
                     /locus_tag="J1Y83_003476"
     CDS             complement(11336..12484)
                     /gene="carA"
                     /locus_tag="J1Y83_003476"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HBA7631591.1"
                     /translation="MIKSALLVLEDGTQFHGRAIGATGSAVGEVVFNTSMTGYQEILT
                     DPSYSRQIVTLTYPHIGNVGTNDADEESSQVHAQGLVIRDLPLIASNFRNTEDLSSYL
                     KRHNIVAIAGIDTRKLTRLLREKGAQNGCIIAGDNPDAALALEKARAFPGLNGMDLAK
                     EVTTAEPYSWTQGSWTLTGGLPEAKKEDELPYHVVAYDFGAKRNILRMLVDRGCRLTI
                     VPAQTSAEDVLKMNPDGIFLSNGPGDPAPCDYAITAIQKFLETDIPVFGICLGHQLLA
                     LASGAKTVKMKFGHHGGNHPVKDVEKNVVMITAQNHGFAVDEATLPANLRVTHKSLFD
                     GTLQGIHRTDKPAFSFQGHPEASPGPHDAAPLFDHFIALIEQYRKTAK"
BASE COUNT         3022 a         3309 c         3377 g         3068 t
ORIGIN      
        1 ttcacccgaa acaaaaatgt gataacaatc acagaatgca gcttattgaa tacccattat
       61 gagttagcca ttaacgcgtc cacgaggtta ataataatta tattaaatgt taacaaaaat
      121 aaaacaaacg ggaacgcaaa ataaatattc gttttcacag tggaattaac tcatgaagaa
      181 tgaaaagaga aaaacgggaa tagaaccgaa ggttttcttt ccgccgttaa taatcgtcgg
      241 catactttgt tggcttacag tcagagatct ggatgcggcg aatgtcgtta ttaatgctgt
      301 attcagttac gtcaccaatg tatggggatg ggcatttgaa tggtatatgg tggtgatgct
      361 tttcggctgg ttctggctgg tgtttggccc gtatgccaaa aagcgtttag gtaacgaacc
      421 accagaattt agtaccgcca gttggatctt tatgatgttc gcctcctgta cgtctgctgc
      481 cgtactgttc tggggatcga ttgagatcta ctactacatc tccaccccgc cgtttggctt
      541 agaaccgaac tcaacaggcg cgaaagagtt ggggctggct tacagcttgt tccactgggg
      601 acctctgccg tgggccactt acagcttcct ttcagtcgcc ttcgcttact tcttctttgt
      661 ccgcaaaatg gaagtgattc gccccagctc caccctggtg ccgctggtag gtgaaaaaca
      721 cgccaaaggg ttgttcggca ctatcgtcga caacttctat ctcgtcgcct tgatcttcgc
      781 gatgggtacc agtctgggcc ttgccacgcc gctggtgacc gagtgtatgc aatggttgtt
      841 tggcattccg cataccctgc aactggacgc tatcatcatt acctgctgga ttatcctcaa
      901 cgccatttgc gtcgcttgcg gtctgcaaaa aggggtacgt atcgccagtg acgtgcgtag
      961 ttacctgagc ttcctgatgc tgggttgggt gttcatcgtc agcggtgcca gcttcatcat
     1021 gaactacttc accgattcgg tggggatgtt gctgatgtat ctgccgcgca tgttgttcta
     1081 taccgatccc atcgctaaag gcggcttccc gcagggctgg accgtgttct actgggcatg
     1141 gtgggtgatt tacgctatcc agatgagtat cttcctcgcc cgcatctccc gtggtcgtac
     1201 cgtgcgtgaa ctgtgcttcg gcatggtgct gggactgaca gcgtcaacct ggatcctgtg
     1261 gactgtactc ggtagtaaca ctctgctgtt gatagataaa aacattatca acattccaaa
     1321 tctgatcgaa cagtacggtg tggcgcgcgc catcatcgaa acctgggccg ctctgccact
     1381 cagcaccgcc accatgtggg gcttcttcat cctctgcttt attgccaccg ttacgctggt
     1441 taacgcctgc tcttataccc tggcgatgtc cacttgccgc gaagtgcgtg acggcgaaga
     1501 acccccgctg ctggtgcgca ttggttggtc agttctggtt ggcattatcg gcattgttct
     1561 gctggcgctc ggcggcctga aaccgattca aaccgccatt atcgccggag gatgcccgct
     1621 gttcttcgtc aacatcatgg tgacgctctc ctttattaaa gacgcgaaac agaactggaa
     1681 agattaatta acccccaaaa tatcaagagg ttgaaagatg gattttaatt taaatgatga
     1741 gcaggaactg tttgtcgccg gtatccgcga actgatggcc agcgaaaact gggaggccta
     1801 ttttgccgag tgcgaccgtg acagcgtcta cccggaacgt tttgtcaaag ccctggcgga
     1861 tatgggtatc gacagtctgc tgatcccgga agaacacggt ggtctggacg cggggtttgt
     1921 tactctcgcc gccgtgtgga tggaactggg tcgtctggga gcgccaactt acgtgctgta
     1981 tcagttaccg ggcgggttta acaccttcct gcgtgaaggt acgcaagagc agatcgacaa
     2041 gatcatggct ttccgcggca ccggtaagca gatgtggaac tcagcaatta ccgaaccggg
     2101 cgcaggctcc gacgtgggta gcctgaaaac gacttatacc cgtagaaatg gtaagattta
     2161 tcttaatggt agtaagtgtt ttattaccag tagcgcctac accccgtaca tcgtggtgat
     2221 ggcgcgcgac ggggcttctc cggacaaacc tgtctacacc gaatggtttg ttgatatgag
     2281 caaaccgggc atcaaagtga ccaaacttga aaagctcggt ctgcgtatgg atagctgctg
     2341 tgaaatcact tttgacgacg tggaactgga cgagaaagac atgttcggtc gggaaggtaa
     2401 cggctttaac cgcgtcaaag aagagttcga ccatgaacgt ttcctggtag ccctgaccaa
     2461 ctacggtacg gcgatgtgcg cctttgaaga tgcggcgcgc tacgccaacc agcgtgtgca
     2521 gtttggcgag gctattggtc gtttccagtt gattcaggaa aaattcgccc acatggcgat
     2581 caaattaaac tccatgaaaa acatgctgta tgaagcagcg tggaaagcag acaacggcac
     2641 catcacctct ggcgatgcag cgatgtgcaa atacttctgc gccaatgcgg catttgaagt
     2701 ggtggatagc gcaatgcagg tgctgggcgg tgtcgggatt gcgggcaacc accgcatcag
     2761 ccgcttctgg cgtgacctgc gtgtagaccg cgtttccgga ggatctgacg aaatgcagat
     2821 cctgacgctg ggtcgcgcgg tgctgaagca ataccgctaa gtccgtgcgc tgcctgatgc
     2881 gacgctgacg cgtcttatca ggcctacggg agcacgaatg taggccggat aaagcgttta
     2941 cgccgcatcc ggcagtcatg cgctacataa atttttcagg agctaataat ggatcatcta
     3001 cccatgccga aattcgggcc gttggccgga ttgcgcgttg tcttctccgg tatcgaaatc
     3061 gccggaccgt ttgccgggca aatgttcgca gaatgggggg cggaagttat ctggatcgag
     3121 aacgtcgcct gggccgacac cattcgcgtt caaccgaact acccgcagct ctcccgccgc
     3181 aatttgcatg cgctgtcgtt aaatattttc aaagatgaag ggcgcgaagc gtttctgaaa
     3241 ttaatggaaa ccaccgatat cttcatcgaa gccagtaaag gtccggcctt tgcccgtcgt
     3301 ggcattaccg atgaagtcct gtggcagcac aacccaaaac tggttatcgc tcatctgtcc
     3361 ggttttggtc agtacggcac cgaggagtac accaatcttc cggcctataa cactatcgcc
     3421 caggccttca gtggttacct gattcagaac ggtgatgttg accagccaat gcctgccttc
     3481 ccgtataccg ccgattactt ttctggcctg actgccacca cggcggcgct ggcagcactg
     3541 cataaagtgc gtgaaaccgg caaaggcgaa agtatcgaca tcgccatgta tgaagtgatg
     3601 ctgcgtatgg gccagtactt catgatggat tacttcaacg gcggcgaaat gtgcccgcgc
     3661 atgaccaaag gtaaagatcc ctactacgcc ggttgcggtc tgtataaatg cgccgacggc
     3721 tacatcgtga tggaactggt gggcattact caaattgcag agtgctttaa agatattggc
     3781 ctcgcacatc tgctcggtac gccggaaatc ccggaaggca ctcagcttat ccaccgtatc
     3841 gaatgccctt acggcccact ggtagaagag aaactcgatg cctggctggc ggcacatacc
     3901 atcgcagaag ttaaagaacg ctttgccgaa ctgaatatcg cctgtgccaa agtactgacc
     3961 gtaccggaac tggaaagcaa tccacagtat gtggctcgcg aatcaatcac tcagtggcaa
     4021 acgatggatg gtcgcacctg caaagggccg aatatcatgc cgaaattcaa aaataacccc
     4081 ggacaaatct ggcgcggaat gccctcacat ggcatggaca cggctgctat tttgaaaaat
     4141 atcggctaca gcgaaaacga cattcaggag ttggtcagca aaggtctggc caaagttgag
     4201 gactaaacgc gtggcgtcag tcagcttgcc tgactagcgc ccgctcaata acaaacgaac
     4261 aaaatggata gaggtgcaat ggatatcatt ggcggacaac atctacgtca aatgtgggac
     4321 gatctggcgg acgtttacgg tcataaaacg gcgctgattt gtgaatccag cagcggagtc
     4381 gttaaccggt atagttatct tgagttaaat caggagatta accgcacggc aaacctgttt
     4441 tatgtgctgg ggattcgcaa aggcgacaag gttgctctac atctcgacaa ctgcccggaa
     4501 tttatctttt gctggttcgg gttggcaaaa attggcgcga ttatggtgcc gattaacgcc
     4561 cgcctgttgc gcgaagaaag cacgtggatc ctgcaaaata gccaggcgtg cctgctggtg
     4621 accagtgcgc aattttatcc tatgtatcaa cagattcagc aggaagatgc ctctcagttg
     4681 cggcacattt gcctgacaga tatggtactt cccgctgatg atggcgtgag ttcgtttact
     4741 caacggaaaa atcaacaacc tgccaccttg tgctatgcac cgccgctatc gactgacgat
     4801 acggcggaaa ttctcttcac ctccggcacc acctcccgac cgaaaggggt ggtgataact
     4861 cattacaacc tgcgcttcgc tggatattac tccgcctggc agtgtgcact gcgtgacgat
     4921 gacgtctacc tgacggtaat gcctgcgttt catatcgatt gccagtgtac tgcggcgatg
     4981 gcggcgtttt ctgccggggc cacctttgtg ctggtcgaga aatacagcgc ccgcgccttc
     5041 tggggacagg tgcagaagta ccacgccacc attaccgaat gtattccgat gatgatccgt
     5101 acgttgatgg tgcagccgcc ttcagcgaac gatcagcaac accgcctgcg ggaagtgatg
     5161 ttttatctca acctgtcgga gcaggaaaaa gatgcgtttt gtgaacgctt cggcgttcgc
     5221 ttgctgacgt cttatgggat gacggaaacc attgttggca ttatcggcga tcgccctggc
     5281 gataaacgac gctggccgtc gattggtcgg gcggggtttt gctacgaagc ggagatccgc
     5341 gacgatcaca atcgcccgct cccggcaggt gaaatcggtg aaatctgtat taaaggcgta
     5401 ccagggaaaa ccatcttcaa agagtatttt ctcaacccga aagccactgc gaaagtgctg
     5461 gaagccgatg gctggctaca taccggcgat accggatacc gcgacgaaga gggctttttt
     5521 tatttcgtcg atcgccgctg caatatgatc aaacgcggtg gcgagaatgt ctcctgcgtg
     5581 gagctcgaaa atatcatcgc cacccatccc aaaattcagg atatcgtggt tgtgggtatt
     5641 aaagattcga ttcgcgatga agccatcaaa gcatttgtgg tgctgaatga aggtgaaaca
     5701 ttgagcgaag aggaattttt ccgcttctgc gaacaaaata tggcgaaatt taaagtgccc
     5761 tcttatctgg agatcagaaa agatctgcca cgtaattgct cggggaaaat aattagaaag
     5821 aatctgaaat aaatgaaacg gcagggaact accctgtcag ctaataatca cgctctaaaa
     5881 caatatgcat tttttgcagg gatgttgtcg tccctgaaaa agcaaaaatg gagaaaagga
     5941 atgagtgaat cattacatct gacccgcaat ggatcaattc tggaaattac ccttgatcgt
     6001 ccaaaagcga atgctattga tgcaaaaacc agctttgaaa tgggcgaagt atttctaaat
     6061 ttccgtgacg atccgcaatt acgtgtcgcc attattaccg gtgccggaga gaaattcttt
     6121 tccgcgggct gggatttaaa agcggcagca gaaggcgaag caccggatgc tgactttggc
     6181 ccgggaggtt ttgcgggatt aaccgaaatt ttcaatctcg acaaaccggt catcgccgct
     6241 gtgaacggct atgcctttgg cggcggcttt gaactggcgc tggcggcaga ttttattgtt
     6301 tgtgccgata acgccagctt cgccctaccg gaagccaaac tgggcatcgt tcctgacagc
     6361 ggcggcgtgc tgcgtctgcc gaagatcctg ccgcctgcca tcgtcaatga aatggtgatg
     6421 accggcagac gaatgggcgc agaagaggcg ctgcgttggg gggtagtcaa ccgcgtggtt
     6481 agccaggcgg aactgatgga taacgcccgc gaactggcgc agcagctggt taacagcgct
     6541 ccgctggcga ttgcggcgct gaaagagatc taccgcacca ccagtgaaat gccggtagaa
     6601 gaagcgtatc gctatattcg cagcggcgtg ttgaaacact atccatcggt tctgcattcg
     6661 gaggatgcca ttgaagggcc gctggcgttt gccgagaagc gcgatccagt gtggaaagga
     6721 cgttaacaac cgtgagctat tacgcctttg aggggttaat tccggttgtt cacccgacgg
     6781 cgtttgtcca tcccagtgca gtcttgattg gcgatgtgat tgtgggagcc ggtgtttaca
     6841 tcggcccact cgcctcactg cgtggtgact acgggcggtt gatcgtgcaa gcgggagcca
     6901 atattcagga tggctgcatt atgcatggct actgcgacac tgacaccatc gttggggaaa
     6961 acggtcatat cgggcacgga gcgatcctgc atggttgtgt gattggtcgc gatgcactgg
     7021 tcgggatgaa cagcgtgatt atggatggcg cggtcattgg cgaagagagc attgttgccg
     7081 ctatgagctt tgtcaaagcg ggctttcgcg gcgagaaacg ccagttgttg atgggtacgc
     7141 cagcccgcgc tgtacgcagc gtgagtgacg acgagttaca ctggaaacgg ttgaatacca
     7201 aagagtatca ggatcttgtc gggcgctgtc atgcagcgtt acatgaaacg cagccactga
     7261 ggcaaatgga ggaaaatcgc ccccgtttgc aggggacgac ggatgtgacg ccgaaacggt
     7321 aacatcgtcg gatgcggcat aaacgcctta tccgacctgg ctgtaaagca accgtaggcc
     7381 ggataagatg cgccagcatc gcatccagcg accaggtcgc acggtatcaa cgaaatgaca
     7441 ttaacgacgc atactctttg acaacatcat ctgccacttc tgctcgcggt taagttccgt
     7501 gagcggaacg gcaggaatac gcggcgtctt tcttacgccg actaatttat cccggctgtt
     7561 attgcgcagg cgcgcgtaaa tttgctcatc gatatcaacc acttttacca gtcgctggca
     7621 ctggcatccc cgcccttcca gcttattagg gatcatctta acttcgcagc ttatttctgt
     7681 gacttccgac agaatataag tcagggtatt aaccgccttg ctgtgttcaa tatcgaaatg
     7741 aatagagatc tctcttgcta tcacccaccg gttttcagcc atcatccatt cggcgattaa
     7801 caaatagagt ggtttttcaa cacatccttc acacatacga actccagttt cacaaattaa
     7861 aagactggct atgactcgtt ttgcagcaat taaacaaggc catattaaaa ttgtggatat
     7921 atcgatagta tgcaacttca ccgtcgataa gtgtgagcta aatcctgctt atgtcatcaa
     7981 aaatatcgat tccccaaaag atttacttaa tgggcaaaaa aaacggtcct catcagagaa
     8041 ccgtattagt tattcgatca aattagcgga tgaaaaatat ccgccatgac acgctattat
     8101 ttgatctgcg cgtgcatttc ctgcaccgaa attacttttt cggtcgcatc ggcattcagc
     8161 gccatcgcgg tggcaaagcc gccgttcagg gtggtgtcgt aatgcacttt atattgcagc
     8221 gcactgcgac ggatcacgcg ggagtcttca atcgcacgac gacctgaggt ggtattgatg
     8281 atgtaggtat attcgccatt cttaatacgg tcctgaatgt gcggacggcc ttcatgcacc
     8341 ttgttcacca gacgcggatt gatacccgct tcgcccagca caatcgccgt gccgtgagtc
     8401 gcatccagct cgaagccctg tttcagcagt tttgccgcca ggtccaccac gcgttcttta
     8461 tcgccttcgc gcacggaaag cagcgcacga ccgtgtttct tcatggtgga gttgctgccc
     8521 agctgcgctt tggcaaacgc ttcagcgaag gtgcggccca cgcccattac ttccccggta
     8581 gagcgcattt ctggccctaa cagcgggtca acgcccggga atttattgaa cggcagcacc
     8641 acttctttca ccgagtagta cggcgggata acttctttgg ttacgccctg ctcagccagc
     8701 gatttgccag ccatcacacg cgccgccact tttgccagcg gtacgccggt ggctttggag
     8761 acgaacggaa cggtacgcgc cgcacgcggg ttaacttcaa tcaggtagac ttcgttgttt
     8821 ttcaccgcaa actgcacgtt catcaggccg cgcacctgca attcgaaggc cagtttctgc
     8881 acctgctggc gcatcacatc ctgaatttcc tgacttaagg tgtaggctgg cagcgaacat
     8941 gcggagtcac cggagtgcac gcccgcctgc tcaatatgct ccatgatgcc gccaatcagt
     9001 accatttcgc cgtcgcagat ggcatccacg tcaacttcta ctgcgtcatc aaggaagtgg
     9061 tccagcaaca ctggcgcatc gttagatacg ctgaccgctg tctggaagta gcgacgcagg
     9121 tcagcttcgt catagacgat ttccatcgcc cgaccgccga gaacgtaaga cggacgcacc
     9181 accagcgggt agccaatctc tttcgctttc tcaaccgcca tttcgatggt ggtaacggtg
     9241 gcgttcgccg gttgtttcag tttcagacgc tcaaccgcat gctggaagcg ttcacggtct
     9301 tccgcacggt cgatggcatc cgggctggta ccgataaccg gtacgccagc agcttccagc
     9361 gcgcgcgcca gtttcagcgg ggtctgaccg ccgtactgga cgataacgcc tttcggcttc
     9421 tcaatacgca caatttccag cacgtcttcc agcgttaccg gttcgaagta gagacggtca
     9481 gaggtgtcgt agtcggtgga gacggtttcc gggttacagt taaccataat ggtttcataa
     9541 ccatcttcgc gcagcgccag cgaggcgtgt acacagcagt agtcgaattc gataccctga
     9601 ccgatacggt tcgggccgcc gccgagcacc atgatttttt cacgatcggt ggacggattc
     9661 gcttcgcact cttcttcata agtggagtac atgtaagcgg tgtcggtggc gaactctgcc
     9721 gcacaagtat ccacgcgctt gtaaaccggg tgcagatcat actggtcgcg caacttgcgg
     9781 atttccgctt cgcgcacgcc cgccagttta gccagacgcg catcggcaaa gcctttgcgt
     9841 ttcaactggc gcaggaattc agcgtgcagg ccagtgatgc ccacttctgc gactttctct
     9901 tccagacgca ccagctcttc aatctgtacc aggaaccagc ggtcaatgtt ggtcaaatta
     9961 aacacgccgt ctacagacag gcccgcgcgg aaggcatcag cgatatacca gatacgctct
    10021 gcgccagcat ctttcagttc gcggcggatt ttagtcagcg cttccgggtc gtcgagactc
    10081 actttcggat cgaagccagt tgcgcccact tccaggccgc gcagcgcttt ttgcagggat
    10141 tcctgctgcg tgcgaccaat cgccatcact tcgccaaccg atttcatctg agtggtcaga
    10201 cggtcgttag caccggcgaa tttctcgaag ttgaagcgag gaattttagt aaccacatag
    10261 tcgatggacg gctcgaagga ggccggagta cgcccaccgg tgatgtcgtt catcagctcg
    10321 tcgagggtgt aacccaccgc cagtttcgcc gccactttgg caatcgggaa accagtggct
    10381 ttcgacgcca gcgcagagga gcgagacacg cgtgggttca tttcgataac aatcaggcga
    10441 ccgtttttcg ggttcaccgc aaactggacg ttggaaccac cggtttcaac gccgatttca
    10501 cgcagtaccg ccatcgaggc gttacgcatg atttgatatt ctttgtcagt cagcgtttgg
    10561 gctggcgcga cagtgatgga gtcaccggtg tggatgccca tcgcatcaaa gttttcgata
    10621 gagcagacga tgatgcagtt gtcgttttta tcacgcacca cttccatctc gtactctttc
    10681 cagccgatca gcgactcatc aatcagcagc tctttagtcg gagaaagatc cagaccgcgg
    10741 gcgcaaattt cttcaaattc ttcacggtta taagcgatac cgccaccgct accgcccatg
    10801 gtaaaggatg ggcgaataat gcacgggaag ccaacttcag cggcaaccgc cagcgcttct
    10861 tccattgaat gagcgatacc ggaacgcgca gtttccagac cgattttctt catcgctacg
    10921 tcgaaacggc ggcggtcttc ggctttatca atcgcatcgg cagtggcacc aatcatggtg
    10981 acgccgaact cttccagcac gccctgacgc tcaagctcca gcgcacagtt cagcgccgtc
    11041 tgccccccca tggttggcag caccgcgtcc ggacgctctt tttcaataat cttgcgtacc
    11101 acttcccagt gaatcggctc gatgtaggtg gcatcagcca tttccgggtc ggtcatgatg
    11161 gtcgccgggt tggagttcac cagaatgacg cggtaaccct cttcacgcag ggctttacac
    11221 gcttgcgcgc cagagtagtc aaactcacac gcctgaccga taacaatcgg gcccgcaccc
    11281 agaatcagga tactttttat atctgtacgt tttggcatgg ctcttttact cctgattact
    11341 tagcggtttt acggtactgc tcaattaacg cgataaagtg gtcaaacaac ggcgcggcgt
    11401 cgtgtggacc agggctggct tccgggtgtc cctggaagct gaacgccggt ttatcggtac
    11461 gatgaatgcc ctgtaacgta ccgtcgaaca gggatttatg tgtgacacgc aggtttgcag
    11521 gtaatgttgc ttcatccacc gcaaaaccgt ggttctgggc ggtgatcatc accacatttt
    11581 tctcaacatc tttaaccgga tggttgccgc cgtggtgacc aaatttcatt ttgacagtct
    11641 tcgcaccgct cgccagcgcc agcagctgat gaccgaggca gatgccgaat accggaatat
    11701 cagtttcgag gaatttctgg atagcggtaa tggcgtaatc gcacggggcc gggtcgccag
    11761 gaccgttgga gaggaagatg ccgtccggat tcattttcag cacatcttcc gcagaagttt
    11821 gcgccggaac gatggtcagg cgacagcctc tatccaccag catacgcagg atgttgcgct
    11881 tggcaccgaa atcgtaggcc acgacgtggt acggcagctc gtcttctttt ttcgcttctg
    11941 gcaggccgcc ggtcagtgtc cagctccctt gtgtccagct atagggttct gcggtggtta
    12001 cttcttttgc cagatccatg ccgttcagac ctgggaacgc gcgggctttt tctaatgcca
    12061 gcgccgcatc cgggttatcg cctgcgataa tgcagccatt ctgagcacct ttctcacgca
    12121 gtaaacgcgt cagcttacgg gtatcgatac cggcaatcgc cacgatgtta tggcgcttca
    12181 ggtaagaaga gaggtcttcg gtattacgga agttgctggc aatcagcggc aggtcgcgaa
    12241 tcaccagacc ttgggcatga acctgagaag attcttcatc ggcgtcatta gtgccgacat
    12301 tgccaatatg gggataagta agagtaacga tttgacgaga ataggaagga tcagtgagga
    12361 tttcttgata accggtcatt gaagtattga aaacgacttc cccaaccgcc gaacctgttg
    12421 cccctatggc ccgaccgtga aactgggttc cgtcttccag aaccaatagc gctgacttaa
    12481 tcaaaacacc ctccagagaa tattcactca ctttatttgc atattaattc acaatgatga
    12541 cgtaaatcaa tgcaaatctg cttaccgacg aatttctggc aaacggcggc attctggaga
    12601 tatgggagct aaaagtcaac ttaatggcgg gattttttat attattttgt ggctcatgca
    12661 tgttttaaag gcattaagcg acaaaaagat catataacat gttctggaaa acgcttgcgc
    12721 agcaagaaga ttgcatgaaa tcaaaatgag cagaaaaaag tggaccaaat ggtcaa
//
LOCUS       DADSYM010000136        12612 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2415
            SAMN08039579-rid11723173.denovo.137, whole genome shotgun sequence.
ACCESSION   DADSYM010000136 DADSYM010000000
VERSION     DADSYM010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039579
            Sequence Read Archive: SRR6362533
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12612)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12612)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980162.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 03:39:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,484
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 4,347
            CDSs (with protein)               :: 4,347
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 43 of 85
            Pseudo Genes (incomplete)         :: 37 of 85
            Pseudo Genes (internal stop)      :: 19 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12612
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039579-rid11723173.denovo.137"
                     /strain="LGE2415"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8302 N 95.9628 W"
                     /collection_date="2015-08-24"
     gene            complement(128..901)
                     /gene="uxuR"
                     /locus_tag="J1Y83_003477"
     CDS             complement(128..901)
                     /gene="uxuR"
                     /locus_tag="J1Y83_003477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Uxu operon transcriptional regulator"
                     /protein_id="HBA7631592.1"
                     /translation="MKSATSAQRPYQEVGAMIRDLIIKTPYNPGERLPPEREIAEMLD
                     VTRTVVREALIMLEIKGLVEVRRGAGIYVLDSSGSHNTDSPEANVCNDAGPFELLQAR
                     QLLESNIAEFAALQATREDIVKMRQALQLEERELASSAPGSSESGDMQFHLAIAEATH
                     NSMLVELFRQSWQWRENNPMWIQLHSHLDDSLYRKEWLGDHKQILAALIKKDARAAKL
                     AMWQHLENVKQRLLEFSNVDDIYFDGYLFDSWPLDKVDA"
     gene            complement(1115..2575)
                     /locus_tag="J1Y83_003478"
     CDS             complement(1115..2575)
                     /locus_tag="J1Y83_003478"
                     /EC_number="1.1.1.57"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructuronate reductase"
                     /protein_id="HBA7631593.1"
                     /translation="MTTIVDSNLPVARPSWDHSRLESRIVHLGCGAFHRAHQALYTHH
                     LLESTDSDWGICEVNLMPGNDRVLIENLKKQQLLYTVAEKGAESTELKIIGSMKEALH
                     PEIDGCEGILNAMARPQTAIVSLTVTEKGYCADAASGQLDLNNPLIKHDLENPTAPKS
                     AIGYIVEALRLRREKGLKAFTVMSCDNVRENGHVAKVAVLGLAQARDPQLAAWIEENV
                     TFPCTMVDRIVPAATPETLQEIADQLGVYDPCAIACEPFRQWVIEDNFVNGRPDWDKV
                     GAQFVADVVPFEMMKLRMLNGSHSFLAYLGYLGGYETIADTMTNPAYRKAAFALMMQE
                     QAPTLSMPEGTDLNAYATLLIERFSNPSLRHRTWQIAMDGSQKLPQRLLDPVRLHLQN
                     GGSWRHLALGVAGWMRYTQGVDEQGNAIDVVDPMLAEFQKINAQYQGADRVKALLGLS
                     GIFADDLPQNADFVGAVTAAYQQLCERGARECVAAL"
     gene            complement(2656..3840)
                     /gene="uxuA"
                     /locus_tag="J1Y83_003479"
     CDS             complement(2656..3840)
                     /gene="uxuA"
                     /locus_tag="J1Y83_003479"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HBA7631594.1"
                     /translation="MEQTWRWYGPNDPVSLADVRQAGATGVVTALHHIPNGEVWSVEE
                     ILKRKAIVEDAGLVWSVVESVPIHEDIKTHTGNYEQWIANYQQTLRNLAQCGIRTVCY
                     NFMPVLDWTRTDLEYVLPDGSKALRFDQIEFAAFEMHILKRPGAEADYTEEEIAQAAE
                     RFATMSDEDKARLTRNIIAGLPGAEEGYTLDQFRKHLELYKDIDKAKLRENFAVFLKA
                     IIPVAEEVGVRMAVHPDDPPRPILGLPRIVSTIEDMQWMVDTVNSMANGFTMCTGSYG
                     VRADNDLVDMIKQFGPRIYFTHLRSTMREDNPKTFHEAAHLNGDVDMYEVVKAIVEEE
                     HRRKAEGKEDLIPMRPDHGHQMLDDLKKKTNPGYSAIGRLKGLAEVRGVELAIQRAFF
                     SR"
     gene            4180..5523
                     /gene="gntP"
                     /locus_tag="J1Y83_003480"
     CDS             4180..5523
                     /gene="gntP"
                     /locus_tag="J1Y83_003480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate permease GntP"
                     /protein_id="HBA7631595.1"
                     /translation="MHVLNILWVVFGIGLMLVLNLKFKINSMVALLVAALSVGMLAGM
                     DLMSLLHTMKAGFGNTLGELAIIVVFGAVIGKLMVDSGAAHQIAHTLLARLGLRYVQI
                     SVIIIGLIFGLAMFYEVAFIMLAPLVIVIAAEAKIPFLKLAIPAVAAATTAHSLFPPQ
                     PGPVALVNAYGADMGMVYIYGVLVTIPSVICAGLILPKFLGNLERPTPSFLKADQPVD
                     MNNLPSFGVSILVPLIPAIIMISTTIANIWLVKDTPAWEVVNFIGSSPIAMFIAMVVA
                     FVLFGTARGHDMQWVMNAFESAVKSIAMVILIIGAGGVLKQTIIDTGIGDTIGMLMSH
                     GNISPYIMAWLITVLIRLATGQGVVSAMTAAGIISAAILDPATGQLVGVNPALLVLAT
                     AAGSNTLTHINDASFWLFKGYFDLSVKDTLKTWGLLELVNSVVGLIIVLIISMVA"
     gene            complement(5768..6670)
                     /gene="fimH"
                     /locus_tag="J1Y83_003481"
     CDS             complement(5768..6670)
                     /gene="fimH"
                     /locus_tag="J1Y83_003481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria D-mannose specific adhesin FimH"
                     /protein_id="HBA7631596.1"
                     /translation="MKRVITLFAVLLMGWSVNAWSFACKTANGTAIPIGGGSANVYVN
                     LAPAVNVGQNLVVDLSTQIFCHNDYPETITDYVTLQRGSAYGGVLSSFSGTVKYSGSS
                     YPFPTTSETPRVVYNSRTDKPWPVALYLTPVSSAGGVAIKAGSLIAVLILRQTNNYNS
                     DDFQFVWNIYANNDVVVPTGGCDVSARDVTVTLPDYPGSVPIPLTVYCAKSQNLGYYL
                     SGTTADAGNSIFTNTASFSPAQGVGVQLTRNGTIIPANNTVSLGAVGTSAVSLGLTAN
                     YARTGGQVTAGNVQSIIGVTFVYQ"
     gene            complement(6690..7193)
                     /gene="fimG"
                     /locus_tag="J1Y83_003482"
     CDS             complement(6690..7193)
                     /gene="fimG"
                     /locus_tag="J1Y83_003482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria minor subunit FimG"
                     /protein_id="HBA7631597.1"
                     /translation="MKWRKRGYLLAAMLAFASATIQAADVTITVNGKVVAKPCTVSTT
                     NATVDLGDLYSFSLMSAGAASAWHDVALELTNCPVGTSRVTASFSGAADSTGYYKNQG
                     TAQNIQLELQDDSGNTLNTGATKTVQVDDSSQSAHFPLQVRALTVNGGATQGTIQAVI
                     SITYTYS"
     gene            complement(7206..7736)
                     /gene="fimF"
                     /locus_tag="J1Y83_003483"
     CDS             complement(7206..7736)
                     /gene="fimF"
                     /locus_tag="J1Y83_003483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria minor subunit FimF"
                     /protein_id="HBA7631598.1"
                     /translation="MRNKPFYLLCAFLWLAVSHALAADSTITIRGYVRDNGCSVAAES
                     TNFTVDLMENAAKQFNNIGATTPVVPFRILLSPCGNAVSAVKVGFTGVADSHNANLLA
                     LENTVSAAAGLGIQLLNEQQNEIPLNAPSSAISWTTLTPGKPNTLNFYARLMATQVPV
                     TAGHINATATFTLEYQ"
     gene            complement(7746..10382)
                     /gene="fimD"
                     /locus_tag="J1Y83_003484"
     CDS             complement(7746..10382)
                     /gene="fimD"
                     /locus_tag="J1Y83_003484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis usher protein FimD"
                     /protein_id="HBA7631599.1"
                     /translation="MSYLNLRLYQRNTQCLHIRKHRLAGFFVRLFVACAFAAQAPLSS
                     AELYFNPRFLADDPQAVADLSRFENGQELPPGTYRVDIYLNNGYMATRDVTFNTGDSE
                     QGIVPCLTRAQLASMGLNTASVSGMNLLADDACVPLTSMIHDATAHLDVGQQRLNLTI
                     PQAFMSNRARGYIPPELWDPGINAGLLNYNFSGNSVQNRIGGNSHYAYLNLQSGLNIG
                     AWRLRDNTTWSYNSSDSSSGSKNKWQHINTWLERDIIPLRSRLTLGDGYTQGDIFDGI
                     NFRGAQLASDDNMLPDSQRGFAPVIHGIARGTAQVTIKQNGYDIYNSTVPPGPFTIND
                     IYAAGNSGDLQVTIKEADGSTQIFTVPYSSVPLLQREGHTRYSITAGEYRSGNAQQEK
                     PRFFQSTLLHGLPAGWTIYGGTQLADRYRAFNFGIGKNMGALGALSVDMTQANSTLPD
                     DSQHDGQSVRFLYNKSLNESGTNIQLVGYRYSTSGYFNFADTTYSRMNGYNIETQDGV
                     IQVKPKFTDYYNLAYNKRGKLQLTVTQQLGRTSTLYLSGSHQTYWGTSNVDEQFQAGL
                     NTAFEDINWTLSYSLTKNAWQKGRDQMLALNVNIPFSHWLRSDSKSQWRHASASYSMS
                     HDLNGRMTNLAGVYGTLLGDNNLSYSVQTGYAGGGDGNSGSTGYATLNYRGGYGNANI
                     GYSHSDDIKQLYYGVSGGVLAHANGVTLGQPLNDTVVLVKAPGAKDAKVENQTGVRTD
                     WRGYAVLPYATEYRENRVALDTNTLADNVDLDNAVANVVPTRGAIVRAEFKARVGIKL
                     LMTLTHNNKPLPFGAMVTSESSQSSGIVADNGQVYLSGMPLAGKVQVKWGEEENAHCV
                     ANYQLPPESQQQLLTQLSAECR"
     gene            complement(10448..11173)
                     /gene="fimC"
                     /locus_tag="J1Y83_003485"
     CDS             complement(10448..11173)
                     /gene="fimC"
                     /locus_tag="J1Y83_003485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418736.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria chaperone FimC"
                     /protein_id="HBA7631600.1"
                     /translation="MSNKNVNVRKSQEITFCLLAGILMFMAMMVAGRAEAGVALGATR
                     VIYPAGQKQVQLAVTNNDENSTYLIQSWVENADGVKDGRFIVTPPLFAMKGKKENTLR
                     ILDATNNQLPQDRESLFWMNVKAIPSMDKSKLTENTLQLAIISRIKLYYRPAKLALPP
                     DQAAEKLRFRRSANSLTLINPTPYYLTVTELNAGTRVLENALVPPMGESAVKLPSDAG
                     SNITYRTINDYGALTPKMTGVME"
     gene            complement(11210..11749)
                     /locus_tag="J1Y83_003486"
     CDS             complement(11210..11749)
                     /locus_tag="J1Y83_003486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418735.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HBA7631601.1"
                     /translation="MKRKRLFLLASLLPMFALAGNKWNTTLPGGNMQFQGVIIAETCR
                     IEAGDKQMTVNMGQISSNRFHAVGEDSSPVPFVIHLRECSTVVSERVGVAFHGVADGK
                     NPDVLSVGEGPGIATNIGVALFDDEGNLVPINRPPVNWKRLYSGSTSLHFIAKYRATG
                     RRVTGGIANAQAWFSLTYQ"
     gene            complement(11814..12368)
                     /locus_tag="J1Y83_003487"
     CDS             complement(11814..12368)
                     /locus_tag="J1Y83_003487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HBA7631602.1"
                     /translation="MKIKTLAIVVLSALSLSSTAALADTTPTTVNGGTVHFKGEVVNA
                     ACAVDAGSVDQTVQLGQVRTATLKQAGATSSAVGFNIQLNDCDTSVATKAAVAFLGTA
                     IDSAHPKVLALQSSAAGSATNVGVQILDRTGNELTLDGATFSEQTTLNNGTNTIPFQA
                     RYYATGAATPGAANADATFKVQYQ"
BASE COUNT         2991 a         3322 c         3086 g         3213 t
ORIGIN      
        1 ttattccaaa acaatatatc gtacgtaatg attaatttac gcataatata ttctgcagaa
       61 cataattaac tattcaggga acaatacata gtgcaaagcg gttaaacttt gcaccagaaa
      121 tgagggatca ggcgtcgact ttatccagcg gccatgaatc aaacagatag ccatcgaaat
      181 agatgtcatc aacattagag aactccagca agcgctgctt cacattctcc agatgctgcc
      241 acattgccag cttcgccgct cgggcatctt ttttgattaa cgcggcgagg atctgtttgt
      301 gatcgcccaa ccactctttg cgatacaggc tgtcatccag gtggctgtgc aactggatcc
      361 acattggatt gttttcccgc cactgccagg actgacggaa taactccacc agcatgctgt
      421 tatgcgttgc ttcggcaata gcgagatgga actgcatgtc accgctttcg ctgctgcccg
      481 gcgcgctgga agccagttca cgctcttcca gttgcaatgc ctgacgcatt ttgacgatat
      541 cttcgcgggt agcctgcagg gcggcaaact cggcgatgtt actttccagt aactgacgcg
      601 cctgtaacag ctcaaaagga cccgcatcat tgcaaacgtt ggcctccgga ctgtctgtgt
      661 tgtggctgcc tgagctatca agaacataaa taccggcacc ccggcgcact tccaccagcc
      721 ctttgatctc cagcatgatc agcgcttcac gtaccaccgt ccgcgtgaca tcaagcattt
      781 ctgcaatttc acgctccggc ggcagccgtt cgccaggatt gtacggcgtc tttatgatca
      841 gatcgcggat catcgccccg acttcctggt aaggtctttg cgcagaggtg gcagatttca
      901 tggtgttgtg tccgttaggt gcggttaatc aaattttgcc ggatgcggcg tgaatgcctt
      961 atccggccta caacgggcaa ttttacggca cccgttaatc tcaaactata ccattagcac
     1021 tggatgttgg cacgtacagg tgggcaaaaa aacgaaaact ggtcaaccaa atattagttt
     1081 gattgaccag taagtctgta gggtaatcag ttagttacag cgcagccaca cactcgcgcg
     1141 caccgcgctc acacagctgc tgatatgccg ccgtcacagc gccaacaaag tcggcgttct
     1201 gcggcagatc atcggcaaag ataccgctca ggcccagcag tgctttcacg cggtctgcac
     1261 cctgatactg cgcgttgatc ttctggaact ccgccagcat cgggtcaacc acgtcaatgg
     1321 cattaccctg ctcatccacg ccctgggtgt agcgcatcca gcccgccacg cccagcgcca
     1381 ggtgacgcca gctaccgccg ttttgcaggt gcagacgcac cgggtccagc agacgctgcg
     1441 gtaacttctg gctgccgtcc attgcaatct gccaggtacg gtgacgcaga gacgggttgc
     1501 tgaaacgctc gatcagcagc gtcgcatagg cgttcaggtc cgtaccttcc ggcatcgaca
     1561 gcgttggcgc ttgttcctgc atcatcaggg caaaggccgc tttgcgataa gccgggttag
     1621 tcatggtgtc ggcaatagtt tcatagccgc cgaggtaacc gaggtacgcc aggaaagagt
     1681 ggctaccgtt cagcatacgc agcttcatca tttcgaacgg tacaacatcc gcaacaaact
     1741 gcgcgccgac tttatcccag tccggacgac cattaacgaa gttatcttca attacccact
     1801 gacggaacgg ttcgcaggca atggcgcacg ggtcgtaaac acccagctgg tcagcaattt
     1861 cctgtaaggt ttctggcgtc gccgccggaa cgatgcggtc aaccatggtg cacggaaagg
     1921 tgacgttttc ttcaatccat gccgccagtt gcgggtcacg cgcctgagcc agcccaagaa
     1981 cggcgacctt cgccacatga ccgttttcac gcacgttatc gcaggacatc accgtaaacg
     2041 ctttcagccc tttttcacga cgcagccgca gggcttctac gatgtaacca atcgcagact
     2101 tcggcgcagt cgggttttcg agatcgtgct tgatcagcgg gttattgaga tccaactgac
     2161 cgcttgccgc atcggcacag tagccttttt ccgtgaccgt cagggagaca atcgccgtct
     2221 gcggacgcgc catcgcgttg agaatacctt cgcagccgtc gatttccgga tgcagcgctt
     2281 ctttcatcga accgataatt ttcagctcgg tgctttctgc gcctttttcc gctacggtgt
     2341 acagcagttg ctgttttttc aggttttcga tcagcacgcg gtcgttgcct ggcatcaggt
     2401 taacttcgca gatgccccag tcgctgtcgg tgctttccag cagatgatgg gtatacagcg
     2461 cctggtgcgc gcggtgaaac gccccgcaac cgagatgcac aatgcgtgat tccagacgag
     2521 aatgatccca tgacgggcgg gcaaccggca gattgctgtc aacaatagta gtcatcgtaa
     2581 actccagaat ccgtcttcag agagtgacgg caaggaagaa ggaaccggca cggcgcgcag
     2641 ccatgccggt ggatattaac ggctaaagaa agcgcgctgg atcgccagtt cgacaccgcg
     2701 aacttcggcc aggcctttca gacgaccaat tgcggagtaa cctgggttgg ttttcttctt
     2761 caggtcgtcc agcatctgat gaccgtggtc cggacgcatc gggatcaggt cttctttgcc
     2821 ttccgcttta cgacggtgtt cttcttcaac aatcgctttc accacttcgt acatatcaac
     2881 gtcaccgttc aggtgcgctg cttcgtggaa ggttttcggg ttatcttcac gcatggtgga
     2941 gcgcagatgg gtgaagtaaa tacgcggacc gaactgcttg atcatatcaa ccagatcgtt
     3001 gtcagcacgc acgccgtagg aaccggtgca catggtgaaa ccgttcgcca tgctgtttac
     3061 ggtatcaacc atccactgca tatcttcaat ggtggaaaca atgcgcggca ggccgaggat
     3121 cgggcgcggc ggatcgtccg ggtgaacagc catacggacg ccaacttctt cggcaaccgg
     3181 aataatcgct ttcaggaaga cggcgaagtt ttcgcgcagt ttggctttgt cgatatcttt
     3241 gtatagctcc aggtgcttac ggaactggtc gagggtatac ccttcttccg cacctggcag
     3301 accggcaata atgttacggg tcagacgcgc tttgtcttcg tcgctcatag tggcgaagcg
     3361 ttcagcggcc tgagcaattt cttcttcggt gtaatccgct tccgcgcctg gacgcttcag
     3421 gatatgcatt tcgaatgcag cgaattcgat ctggtcgaag cgcagagctt tggagccgtc
     3481 tggcagcacg tattcgaggt ctgtacgagt ccagtcgagc accggcatga agttgtagca
     3541 cacggtgcga atgccgcact gcgccaggtt gcgcagggtc tgctgatagt tagcaatcca
     3601 ctgctcatag ttgccagtgt gggttttgat atcttcgtga attggtacgc tttctacgac
     3661 agaccatacc aggcctgcgt cttcaacgat cgccttgcgt ttgaggatct cttcaacgga
     3721 ccatacttcg ccgttcggga tatggtgcag cgcggtaacc acgccagttg cgcccgcctg
     3781 acggacatca gctaaagaaa ccggatcgtt tgggccgtac cagcgccagg tctgttccat
     3841 gatttttcct ctttcacata gtgggttcaa cagcgaagag acgcggaacc ccggagttgt
     3901 ttaggtatcc ggatgaacaa cccggccaca gccgtagcgt cgcatcggat gagatggcgt
     3961 ataagttcta ccttttggta agacatgtca acccgaattc taaaattggt taaccacatc
     4021 acaagaattt cactctcctg gttgttgctg caaattgcag caaagggtaa tcgatggtta
     4081 ttgatcagag gatgtgacat tcatcgcaac aatggttgac caatttacat aacatgtcgc
     4141 ccaaaataac aacggttcaa ccaggtaagg taaaacgtta tgcatgtgct taacattctc
     4201 tgggtggtat tcggcattgg cctgatgctg gtactgaatt tgaagttcaa aatcaattca
     4261 atggtggcct tgttggtggc ggcgctgtcc gtcgggatgc tggcgggcat ggatttgatg
     4321 tcgctgctgc acaccatgaa agcgggcttc ggcaacacgc tgggggaact ggctatcatc
     4381 gtggtgttcg gtgcggtcat cggtaaattg atggtcgact ccggcgcggc tcaccagata
     4441 gcgcatacgc tgctggcgcg tctcggtctg cgctatgttc agatatcagt gattatcatc
     4501 ggacttattt ttggtctggc gatgttctat gaagtggcct ttattatgct ggcgccgctg
     4561 gtgatcgtaa ttgccgccga agccaaaatc ccgttcctga aactggctat tccggcagta
     4621 gcggcggcca ccaccgcaca ttcactgttc cctccgcaac cgggtccggt ggcgctggtg
     4681 aatgcttacg gcgcagatat ggggatggtt tatatctatg gcgtcctggt gactattccc
     4741 agcgtaatct gtgcgggact gatcttgccg aaattcctcg gcaaccttga gcgcccgaca
     4801 ccgtcatttc tgaaagcgga tcaaccggtt gatatgaaca acctgccctc ttttggtgtc
     4861 tcgattctgg tgccgctgat cccggcgatc attatgatct ccaccaccat cgccaatatc
     4921 tggctggtaa aagatacccc tgcctgggaa gtggttaact ttatcggttc ctcgccgatt
     4981 gcaatgttta ttgcgatggt ggttgcattc gtactctttg gtaccgcgcg tggtcatgac
     5041 atgcagtggg tgatgaacgc ttttgaaagc gcggtgaaaa gcattgcaat ggtgattctg
     5101 atcatcggtg caggtggcgt gctgaagcag accatcatcg acaccggcat cggcgacacc
     5161 atcggcatgc tgatgtccca cggcaatatc tcgccctaca tcatggcatg gctgatcacc
     5221 gtactgattc gtctggcgac gggtcagggt gtggtttcag cgatgaccgc cgccgggatt
     5281 atcagtgcag caatccttga tccagcaact ggtcagctgg ttggcgtgaa tccggcgctg
     5341 ctggtactgg cgacggctgc gggttccaac accctcaccc acattaacga tgcctcattc
     5401 tggttgttca aaggttactt tgacctgtca gtaaaagaca cgttgaaaac ctggggtctg
     5461 ctggagctgg tcaactccgt ggttgggctg attattgtgc tgattattag catggtagcg
     5521 taaaagaaca ccagatcctg ccattggcag gctctgactg atgacaaagt ctaaaaatgt
     5581 gcccggccag tcccctcgcc cctccgggga gagggttagg gtgaggggaa cagaccagca
     5641 aagtgcgctt tgtttatgcc agatgcgacg ctggcgcatc tggcctacaa agggctaacg
     5701 tgcaggtttt gtaggtctga tagcgtagcg cctcagggac cagcattagc aatgtcctgt
     5761 gatttcttta ttgataaaca aaagtcacgc caataatcga ttgcacattc cctgcagtca
     5821 cctgccctcc ggtacgtgcg taatttgccg ttaatcccag acttaccgcc gaagtcccta
     5881 ctgctcctaa cgataccgtg ttattcgctg gaataatcgt accgttgcgc gtcaactgta
     5941 cgccgacgcc ctgcgcgggt gaaaacgacg cggtattggt gaaaatcgag ttgcccgcat
     6001 ctgcggttgt gccggagagg taatacccca ggttttggct tttcgcacaa taaacggtaa
     6061 gaggaatcgg cactgaacca gggtagtccg gcagagtaac ggtgacatca cgagcagaaa
     6121 catcgcagcc gccagtgggc accaccacat cattattggc gtaaatattc cacacaaact
     6181 gaaaatcatc gctgttatag ttgttggtct gtcgcaaaat aagcacggca attaatgaac
     6241 cagctttaat cgccaccccg cccgcactgc tcacaggcgt caaataaagc gccaccggcc
     6301 acggcttatc cgttctcgaa ttataaacaa cccgcggcgt ttcactggtg gtaggaaatg
     6361 gatagctact gccactatat tttacggtcc cggaaaaact agataacacg ccgccataag
     6421 ccgagcctcg ttgcagtgtg acataatctg taatggtttc cgggtaatcg ttatggcaaa
     6481 agatttgcgt cgaaagatcc acgaccaggt tttgccccac attcacggca ggcgcaaggt
     6541 ttacataaac attggcgctg ccaccgccaa tagggattgc ggtaccattg gcggttttac
     6601 aggcgaatga ccaggcattt accgaccagc ccatcagcag tacagcaaac agggtaataa
     6661 ctcgtttcat tacaatcatc tctttgggtt cagctgtagg tataggtgat gctaatcact
     6721 gcctgaatgg ttccctgagt ggctccgcca tttaccgtca atgctctgac ctgtaacggg
     6781 aagtgcgctg attgtgagga atcatccacc tgaactgttt tggttgcgcc agtattcaat
     6841 gtgttgccac tgtcatcctg tagctctaac tggatgtttt gcgcggtccc ctgattttta
     6901 taatatccgg tactgtcggc tgccccgctg aagctggcag tgaccctcga cgttcccacc
     6961 ggacaattag tcaactcaag cgcaacatca tgccaggccg atgccgcccc ggcagacatc
     7021 agactgaaag aataaagatc gccgagatca accgtggcat tggtggtgga aaccgtgcac
     7081 ggtttggcga cgaccttacc gttcaccgtg atggtgacat cggctgcctg tatcgtcgca
     7141 cttgcgaacg ccaacattgc cgccaataaa tacccacgtt tgcgccattt catgagcatc
     7201 tccagttact gatattcaag agtgaaggta gccgtggcat tgatatgccc cgcagtgaca
     7261 ggcacctgtg tcgccattag ccgggcgtaa aaattcaacg tatttggttt acccggcgtc
     7321 agggtcgtcc acgaaatcgc ggacgatggg gcattaaggg gtatctcatt ttgctgctca
     7381 ttcagaagct gtattcccag tcccgcagcc gctgacaccg tattttcaag tgcaagcagg
     7441 ttggcattgt ggctatctgc aacgccagta aacccaacct ttacggcaga aacagcgtta
     7501 ccacagggtg acagcaaaat acgaaatgga acgacaggag tcgtcgcgcc aatgttgtta
     7561 aattgcttcg ccgcgttttc catcaaatca acagtaaaat tggttgattc agcggccaca
     7621 ctacagccgt tatctctgac atagccgcgg atagtaatcg tgctatccgc agccaaagcg
     7681 tgactcaccg ccagccacaa aaaagcgcac agaagataaa aaggtttgtt tctcatcacg
     7741 cccccttaac gacattcagc tgatagctgg gttaataact gctgctgact ctctggtggc
     7801 agttgataat tggcgacaca gtgagcattt tcctcttctc cccatttcac ctgaactttt
     7861 cccgctaaag gcattccgct gaggtaaacc tgaccattat ccgcaacaat gccgctactc
     7921 tggctactct ctgatgtcac catcgcccca aacggcagcg gcttattatt gtgggttagc
     7981 gtcatgagca gttttatccc aacgcgcgct ttaaactctg ctcgcacgat cgccccacgg
     8041 gtgggaacaa cgttagcgac cgcattatct aaatcgacgt tatcagccag ggtattggta
     8101 tccagcgcca ctctgttttc cctatattca gtggcataag gcagcacggc ataaccccgc
     8161 cagtcggtac gcactcctgt ctggttttcg acttttgcat cttttgcgcc aggcgcttta
     8221 acaagcacta ccgtatcgtt taacggctgc cccagcgtta cgccattggc atgagccagt
     8281 accccaccgc tgactccgta atagagctgc ttaatatcat cgctatggct gtaaccgatg
     8341 ttggcattgc cgtagccacc gcgataattc agcgtggcgt agcctgtact tccgctatta
     8401 ccatcgcctc ccccggcata gccggtttgc acgctataac tgaggttgtt gtctcccagc
     8461 aacgtaccgt atacaccggc cagattggtc atccgaccgt tgagatcgtg tgacatgctg
     8521 tagctggcac tggcatgtcg ccactgagat ttactgtcag aacgcagcca gtggctgaaa
     8581 ggaatattga cgttaagagc taacatctga tcccgtcctt tttgccaggc gtttttcgtc
     8641 aggctatagc tgagcgtcca gttaatatct tcgaacgcag tatttaatcc agcctggaat
     8701 tgctcatcga cattactcgt tccccaataa gtttgatggc taccactcaa atacagtgtt
     8761 gatgtgcgcc cgagttgctg agtaacggtg agttgtaatt tcccgcgttt gttataagcg
     8821 agattgtaat agtcggtgaa tttcggctta acctgaataa ctccatcctg tgtttcgatg
     8881 ttgtagccat tcattcgact gtatgttgta tcagcgaaat taaaataccc actggtcgaa
     8941 taacggtaac ccactaactg aatattcgtg cctgactcat tgagcgattt gttataaaga
     9001 aaacgcaccg attgtccgtc atgctgactg tcatcgggaa gtgtggaatt agcctgcgtc
     9061 atatccacag acagagcgcc cagtgccccc atatttttcc cgataccgaa attaaaagca
     9121 cgataacgat ccgccagttg cgttccacca tatattgtcc agccagccgg aaggccgtgg
     9181 agtaatgtac tctggaaaaa gcggggtttt tcctgttgcg catttccact acggtattct
     9241 cctgccgtaa tggaataacg tgtatgccct tcacgttgca aaagcgggac tgacgaatag
     9301 ggtacggtaa aaatctgcgt gctgccgtca gcctctttaa tcgttacctg caagtcacca
     9361 ctattacctg cggcatagat atcgttgatg gtaaaaggcc ccggtggcac cgtactatta
     9421 taaatgtcat acccattttg tttaatagtg acctgtgcag taccacgagc aataccgtgg
     9481 atcaccgggg caaatcctct ttggctatcg ggtaacatat tgtcatctga ggccaattgt
     9541 gcgccgcgaa agttaatacc atcgaaaata tcgccctgag tataaccatc acccagcgtc
     9601 agccgggaac gtaacggtat tatgtctcgc tcaagccagg tattgatatg ctgccattta
     9661 tttttgctac ctgatgaact gtcgctactg ttataactcc aggtggtatt gtcgcgtaaa
     9721 cgccacgcac caatatttaa cccactctgt aggtttaaat atgcataatg gctgttaccc
     9781 ccaatccgat tctgtacact atttccgctg aaattataat tgagcaatcc ggcattaata
     9841 ccgggatccc ataactcagg aggaatataa ccacgcgcgc gattactcat aaatgcctga
     9901 gggatcgtca ggttgagtcg ctgctgacca acatctaaat gcgcagtagc gtcatggatc
     9961 attgaggtta atggcacgca ggcatcatcc gccagcagat tcataccgga gacagaagcc
    10021 gtattcagcc ccatactggc gagttgcgcg cgtgtcaggc agggaacaat cccttgttcg
    10081 ctgtcacccg tattaaatgt gacatcacgc gttgccatat aaccattatt caaatagata
    10141 tcaacacgat acgtccctgg cggtaattct tgcccatttt caaaacgcga taaatcagcc
    10201 acagcctggg gatcatccgc taaaaaacgc gggttaaaat agagttcggc agatgacaaa
    10261 ggtgcctgtg cggcaaaagc acaggcaaca aagagtcgga caaaaaaacc agccaaacga
    10321 tgcttacgaa tatgcaagca ttgtgtgttt cgctggtaaa gtcttaaatt cagatatgac
    10381 attattcctc cggtttaaat cacccggcag tcaattcttt ttattcaggc gaaaaaatcc
    10441 ccctgcgtta ttccattacg cccgtcattt tgggggtaag tgcgccataa tcatttattg
    10501 ttcggtaagt aatattgctt cctgcatcag aaggcaattt aaccgcgctt tcgcccattg
    10561 gaggcaccaa tgcattttca agaacccgag ttccggcatt caactctgtt accgtcaggt
    10621 aatagggtgt cgggttgatc agcgtcagag aattcgcgct acgacgaaat cttaattttt
    10681 ctgcggcctg atcgggtggc aacgctaatt tagccggacg atagtacagt ttaatgcggc
    10741 tgataattgc gagctgtagc gtattctcag tcaattttga tttatccatt gacggaatcg
    10801 ctttaacatt catccagaat aaactttccc gatcctgtgg caattggtta tttgttgcat
    10861 caagaatacg caaggtattc tctttttttc ccttcatcgc aaacagaggt ggcgtcacga
    10921 taaaacggcc atcctttaca ccatcggcat tttctaccca tgattgaatt aaataggtgc
    10981 tattttcatc attatttgtc acggcaagtt gcacttgttt ttgccctgcc ggataaatta
    11041 cgcgagtcgc acctaaggcc actcccgctt cagcgcgtcc ggcaaccatc attgccatga
    11101 acatcaggat acctgccagc aagcagaatg ttatttcctg cgatttcctt acattgacgt
    11161 ttttattact cactgtcctg ttcctgttat cacattatct gctgaacaat tactgatagg
    11221 ttaaagagaa ccaggcctgg gcattggcga tgccgccagt aacccgacgc ccggtagcac
    11281 gatatttggc gatgaaatgt agcgaagtag agcctgagta aagccgtttc cagtttactg
    11341 gaggacgatt aatcggtacg aggtttcctt catcatcaaa caacgctacg ccaatattgg
    11401 tggctatccc tggcccctct cccacggaaa gcacatccgg atttttacca tccgcgacac
    11461 cgtgaaacgc cacacccaca cgttcactca ccaccgtgct acattcccgt aaatgaataa
    11521 caaaaggcac cggtgagcta tcttccccta ccgcatgaaa ccggttactg ctgatttgcc
    11581 ccatattgac cgtcatttgt ttatcgccgg cttcaatccg gcaagtttcc gcaataatga
    11641 cgccctgaaa ttgcatattt ccgccgggca gcgtggtatt ccatttattt ccggccagag
    11701 caaacattgg cagcaacgac gctaatagaa ataatctctt ccttttcatc gttattttta
    11761 tcgcacaagg gtgggcatcc ctgcccgtaa tgacgtccct gaacctgggt aggttattga
    11821 tactgaacct tgaaggtcgc atccgcatta gcagcacctg gggttgcggc accggttgca
    11881 taataacgcg cctggaacgg aatggtgtta gtaccgttat tcagggttgt ttgctcactg
    11941 aatgtcgcac cgtccagcgt cagctcattg cctgttctgt ccagaatctg cacgccaaca
    12001 tttgttgcgc tacccgcagc tgaactctgc agagccagta ctttaggatg agcactgtca
    12061 atcgcagtcc ccaagaaggc aacagcggct tttgtggcaa cactggtatc gcaatcattc
    12121 agctgaatgt taaaaccgac agcagagctg gttgctccag cctgcttcaa agtggcggta
    12181 cgaacctggc ccaactgaac ggtttgatca acagagcctg catcaactgc gcaagcggcg
    12241 ttaacaactt cccctttaaa gtgaacggtc ccaccattta ccgtcgtggg tgtagtatca
    12301 gccagagccg ctgtagaact cagggacaga gccgacagaa caacaattgc cagagtttta
    12361 attttcatac tgctttcctt tcaaaaaact atttctaaat cgacatgggc agtcgttctg
    12421 tacactttgt tttgtcaacg agtttgactg ccaacactgc acagttttcc cccaaaagat
    12481 gaaacatttg gggccatttt gactcataga ggaaagcatc gcgcacaaac tttttcagtt
    12541 tatttgttgg cttaatgttc tatggttatc tttatttgca gttttttata ttatatgagg
    12601 tggtttttgg ag
//
LOCUS       DADSYM010000137        12572 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2415
            SAMN08039579-rid11723173.denovo.138, whole genome shotgun sequence.
ACCESSION   DADSYM010000137 DADSYM010000000
VERSION     DADSYM010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039579
            Sequence Read Archive: SRR6362533
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12572)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12572)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980162.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 03:39:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,484
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 4,347
            CDSs (with protein)               :: 4,347
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 43 of 85
            Pseudo Genes (incomplete)         :: 37 of 85
            Pseudo Genes (internal stop)      :: 19 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12572
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039579-rid11723173.denovo.138"
                     /strain="LGE2415"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8302 N 95.9628 W"
                     /collection_date="2015-08-24"
     gene            81..335
                     /gene="bssS"
                     /locus_tag="J1Y83_003488"
     CDS             81..335
                     /gene="bssS"
                     /locus_tag="J1Y83_003488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415578.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator BssS"
                     /protein_id="HBA7631603.1"
                     /translation="MEKNNEVIQTHPLVGWDISTVDSYDALMLRLHYQTPNKSEQEGT
                     EVGQTLWLTTDVARQFISILEAGIAKIESGDFQVNEYRRH"
     gene            450..1568
                     /gene="solA"
                     /locus_tag="J1Y83_003489"
     CDS             450..1568
                     /gene="solA"
                     /locus_tag="J1Y83_003489"
                     /EC_number="1.5.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-methyl-L-tryptophan oxidase"
                     /protein_id="HBA7631604.1"
                     /translation="MKYDLIIIGSGSVGAAAGYYATRAGLNVLMTDAHMPPHQHGSHH
                     GDTRLIRHAYGEGEKYVPLVLRAQMLWDELSRHNEDDPIFVRSGVINLGPADSAFLAN
                     VAHSAEQWQLNVEKLNAQGIMARWPEIRVPDNYIGLFETDSGFLRSELAIKTWIQLAK
                     EAGCAQLFNCPVTAIRHDDDGVTIETADGEYQAKKAIVCAGTWVKDLLPELPVQPVRK
                     VFAWYQADGRYSVKNKFPAFTGELPNGDQYYGFPAENDALKIGKHNGGQVIHSADERV
                     PFAEVVSDGSEAFPFLRNVLPGIGCCLYGAACTYDNSPDEDFIIDTLPGHDNTLLITG
                     LSGHGFKFASVLGEIAADFAQDKKSDFDLTPFRLSRFQ"
     gene            1616..1729
                     /locus_tag="J1Y83_003490"
     CDS             1616..1729
                     /locus_tag="J1Y83_003490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2770 domain-containing protein"
                     /protein_id="HBA7631605.1"
                     /translation="MRRLVNYLINNIREHLMLYLFLWGLLAIVDLIYVFYF"
     gene            1991..2557
                     /locus_tag="J1Y83_003491"
     CDS             1991..2557
                     /locus_tag="J1Y83_003491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
                     /protein_id="HBA7631606.1"
                     /translation="MSFTNTPERYGVISAALHWLSAIIVYGMFALGLWMVTLSYYDGW
                     YHQAPELHKSIGILLMMGLVIRVLWRVISPPPGPLPSYSPMTRLAAKAGHLALYLLLF
                     AIGISGYLISTADGKPISVFGWFDVPATLSDAGAQADFAGALHFWLAWSVVVLSVMHG
                     FMALKHHFIDKDDTLKRMLGKSSSDYGV"
     gene            2561..3136
                     /locus_tag="J1Y83_003492"
     CDS             2561..3136
                     /locus_tag="J1Y83_003492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HBA7631607.1"
                     /translation="MKKSLLGLTFASLMFSAGSAVAADYKIDKEGQHAFVNFRIQHLG
                     YSWLYGTFKDFDGTFTFDEKNPAADKVNVTINTTSVDTNHAERDKHLRSADFLNTAKY
                     PQATFTSTSVKKDGDELDITGDLTLNGVTKPVTLEAKLIGQGDDPWGGKRAGFEAEGK
                     IKLKDFNIKTDLGPASQEVDLIISVEGVQQK"
     gene            complement(3178..4230)
                     /locus_tag="J1Y83_003493"
     CDS             complement(3178..4230)
                     /locus_tag="J1Y83_003493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706977.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HBA7631608.1"
                     /translation="MPVLHNRISNDALKAKMLAESEPRTTISFYKYFHIADPKVTRDA
                     LYQLFTALNVFGRVYLAHEGINAQISVPASNVETFRAQLYAFDPALEGLRLNIALDDD
                     GKSFWVLRMKVRDRIVADGIDDPHFDASNVGEYLQAAEVNAMLDDPDALFIDMRNHYE
                     YEVGHFENALEIPADTFREQLPKAVEMMQAHKDKKIVMYCTGGIRCEKASAWMKHNGF
                     NKVWHIEGGIIEYARKARDQGLPVRFIGKNFVFDERMGERISDEIIAHCHQCGAPCDS
                     HTNCKNDGCHLLFIQCPVCAEKYKGCCSEICCEESALPPDEQRRRRAGRENGNKIFNK
                     SRGRLNTTLGIPDPTE"
     gene            4455..5375
                     /locus_tag="J1Y83_003494"
     CDS             4455..5375
                     /locus_tag="J1Y83_003494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LpxL/LpxP family Kdo(2)-lipid IV(A)
                     lauroyl/palmitoleoyl acyltransferasee"
                     /protein_id="HBA7631609.1"
                     /translation="MTNLPKFSTALLHPRYWLTWLGIGVLWLVVQLPYPVIYRLGCGL
                     GKLALRFMKRRAKIVHRNLELCFPEMSEQERRKMVVKNFESVGMGLMETGMAWFWPDR
                     RIARWTEVIGMEHIRDVQAQKRGILLVGIHFLTLELGARQFGMQEPGIGVYRPNDNPL
                     IDWLQTWGRLRSNKSMLDRKDLKGMIKALKKGEVVWYAPDHDYGPRSSVFVPLFAVEQ
                     AATTTGTWMLARMSGACLVPFVPRRKPDGKGYQLIILPPECSPPLDDAETTAAWMNKV
                     VEKCIMMALEQYMWLHRRFKTRPEGVPSRY"
     gene            5547..6773
                     /gene="mdtG"
                     /locus_tag="J1Y83_003495"
     CDS             5547..6773
                     /gene="mdtG"
                     /locus_tag="J1Y83_003495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtG"
                     /protein_id="HBA7631610.1"
                     /translation="MSPCENDTPINWKRNLIVAWLGCFLTGAAFSLVMPFLPLYVEQL
                     GVTGHSALNMWSGIVFSITFLFSAIASPFWGGLADRKGRKIMLLRSALGMGIVMVLMG
                     LAQNIWQFLILRALLGLLGGFVPNANALIATQVPRNKSGWALGTLSTGGVSGALLGPM
                     AGGLLADSYGLRPVFFITASVLILCFFVTLFCIREKFQPVSKKEMLHMREVVTSLKNP
                     KLVLSLFVTTLIIQVATGSIAPILTLYVRELAGNVSNVAFISGMIASVPGVAALLSAP
                     RLGKLGDRIGPEKILITALIFSVLLLIPMSYVQTPLQLGILRFLLGAADGALLPAVQT
                     LLVYNSSNQIAGRIFSYNQSFRDIGNVTGPLMGAAISANYGFRAVFLVTAGVVLFNAV
                     YSWNSLRRRRIPQISN"
     gene            6856..7230
                     /locus_tag="J1Y83_003496"
     CDS             6856..7230
                     /locus_tag="J1Y83_003496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309456.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secY/secA suppressor protein"
                     /protein_id="HBA7631611.1"
                     /translation="MTMYATLEEAIDAAREEFLADNPGIDAENANVQQFNAQKYVLQD
                     GDIMWQVEFFADEGEEGECLPMLSGEAAQSVFDGDYDEIEIRQEWQDENTLHEWDEGE
                     FQLEPPLDTEEGRTAADEWDER"
     gene            complement(7231..7458)
                     /locus_tag="J1Y83_003497"
     CDS             complement(7231..7458)
                     /locus_tag="J1Y83_003497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceK/YidQ family lipoprotein"
                     /protein_id="HBA7631612.1"
                     /translation="MRLIVMSIMVTLLSGCGSIISRTIPGQGHGNQYYPGVQWDVRDS
                     AWRYVTILDLPFSLVFDTLLLPIDIHHGPYE"
     gene            complement(7630..10173)
                     /gene="mdoH"
                     /locus_tag="J1Y83_003498"
     CDS             complement(7630..10173)
                     /gene="mdoH"
                     /locus_tag="J1Y83_003498"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis glucosyltransferase MdoH"
                     /protein_id="HBA7631613.1"
                     /translation="MNKTTEYIDAMPIAASEKAALPKTDIRAVHQALDAEHRTWARED
                     DSPQGSVKARLEQAWPDSLADGQLIKDDEGRDQLKAMPEVKRSSMFPDPWRTNPVGRF
                     WDRLRGRDVTPRYLARLTKEEQESEQKWRTVGTIRRYILLILTLAQTVVATWYMKTIL
                     PYQGWALINPMDMVGQDVWVSFMQLLPYMLQTGILILFAVLFCWVSAGFWTALMGFLQ
                     LLIGRDKYSISASTVGDEPLNPEHRTALIMPICNEDVNRVFAGLRATWESVKATGNAK
                     HFDVYILSDSYNPDICVAEQKAWMELIAEVGGEGQIFYRRRRRRVKRKSGNIDDFCRR
                     WGSQYSYMVVLDADSVMTGDCLCGLVRLMEANPNAGIIQSSPKASGMDTLYARCQQFA
                     TRVYGPLFTAGLHFWQLGESHYWGHNAIIRVKPFIEHCALAPLPGEGSFAGSILSHDF
                     VEAALMRRAGWGVWIAYDLPGSYEELPPNLLDELKRDRRWCHGNLMNFRLFLVKGMHP
                     VHRAVFLTGVMSYLSAPLWFMFLALSTALQVVHALTEPQYFLQPRQLFPVWPQWRPEL
                     AIALFASTMVLLFLPKLLSILLIWCKGTKEYGGFWRVTLSLLLEVLFSVLLAPVRMLF
                     HTVFVVSAFLGWEVVWNSPQRDDDSTSWGEAFKRHGSQLLLGLVWAVGMAWLDLRFLF
                     WLAPIVFSLILSPFVSVISSRATVGLRTKRWKLFLIPEEYSPPQVLVDTDRFLEMNRQ
                     RSLDDGFMHAVFNPSFNALATAMATARHRASKVLEIARDRHVEQALNETPEKLNRDRR
                     LVLLSDPVTMARLHFRVWNSPERYSSWVSYYEGIKLNPLALRKPDAASQ"
     gene            complement(10166..11701)
                     /gene="mdoG"
                     /locus_tag="J1Y83_003499"
     CDS             complement(10166..11701)
                     /gene="mdoG"
                     /locus_tag="J1Y83_003499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis protein MdoG"
                     /protein_id="HBA7631614.1"
                     /translation="MMKMRWLSAAVMLTLYTSSSWAFSIDDVAKQAQSLAGKGYEAPK
                     SNLPSVFRDMKYADYQQIQFNHDKAYWNNLKTPFKLEFYHQGMYFDTPVKINEVTATA
                     VKRIKYSPDYFTFGDVQHDKDTVKDLGFAGFKVLYPINSKDKNDEIVSMLGASYFRVI
                     GAGQVYGLSARGLAIDTALPSGEEFPRFKEFWIERPKPTDKRLTIYALLDSPRATGAY
                     KFVVMPGRDTVVDVQSKIYLRDKVGKLGVAPLTSMFLFGPNQPSPANNYRPELHDSNG
                     LSIHAGNGEWIWRPLNNPKHLAVSSFSMENPQGFGLLQRGRDFSRFEDLDDRYDLRPS
                     AWVTPKGEWGKGSVELVEIPTNDETNDNIVAYWTPDQLPEPGKEMNFKYTITFSRDED
                     KLHAPDNAWVQQTRRSTGDVKQSNLIRQPDGTIAFVVDFTGAEMKKLPEDTPVTAQTS
                     IGDNGEIVESTVRYNPVTKGWRLVMRVKVKDAKKTTEMRAALVNADQTLSETWSYQLP
                     ANE"
     gene            12095..>12572
                     /locus_tag="J1Y83_003500"
     CDS             12095..>12572
                     /locus_tag="J1Y83_003500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HBA7631615.1"
                     /translation="MNPVPAQREYFLDSIRAWLMLLGIPFHISLIYSSHTWHVNSAEP
                     SLWLTLFNDFIHSFRMQVFFVISGYFSYMLFLRYPLKKWWKVRVERVGIPMLTAIPLL
                     TLPQFIMLQYVKGKAESWPGLSLYDKYNTLAWELISHLWFLLVLVVMTTLCVWIFKR"
BASE COUNT         3130 a         3223 c         3054 g         3165 t
ORIGIN      
        1 tggtatactg aagttgcttc agataaaaac gcattgaacc tcgaataacg ttgtctagta
       61 acacgaatta gggggccatg atggaaaaaa ataatgaagt cattcagact catccgctcg
      121 tagggtggga catcagcacc gttgatagct atgatgcgct gatgttgcgt ttgcactacc
      181 agaccccaaa taagtccgag caggaaggga ctgaagttgg tcagacgctc tggttaacca
      241 ctgatgttgc cagacaattt atttcgatat tagaagcagg aatcgccaaa attgaatccg
      301 gtgatttcca ggtaaacgag tatcggcgtc attaacatga catgaccatc cacccaaaag
      361 gcacctcacc ggtgccttta ccatttctgg gttgtatatc tgcattcagt taattactct
      421 gcttaaacca tgagagcaga gagaaaaaaa tgaaatacga tctcatcatt attggcagcg
      481 gttccgtagg cgctgccgcc gggtattatg caacccgcgc cggtttaaac gtgctaatga
      541 ccgacgccca tatgccaccg catcaacacg gcagccacca cggcgatacg cgattaattc
      601 gccatgctta tggtgaaggt gaaaagtatg tcccgctggt cctccgcgcg caaatgctgt
      661 gggatgaact ttcccgccac aacgaagatg atcccatttt tgtacgctct ggtgtcatta
      721 acctcggtcc ggctgactcc gcatttctcg ccaacgtcgc ccacagcgcc gaacagtggc
      781 aactcaacgt tgaaaagctc aacgcgcaag ggattatggc ccgctggcca gaaatacgcg
      841 tcccggacaa ctacatcggt ttatttgaaa ctgattccgg ttttttgcgc agcgaactgg
      901 cgattaaaac ctggatccaa ctggcgaagg aagcgggctg tgcgcagctg ttcaactgcc
      961 cggtcaccgc aattcgtcat gacgatgatg gcgtaactat tgaaacggct gacggtgagt
     1021 atcaggcgaa aaaagcgatt gtctgcgcgg gaacatgggt aaaagaccta ctcccggagc
     1081 tgcctgtcca gcccgtacgc aaagtatttg cctggtatca ggccgatggc cgctatagcg
     1141 tgaagaataa attcccggca tttaccggtg aactgcccaa tggcgatcaa tattatggtt
     1201 ttccggcaga aaacgacgcg ttgaagattg gcaaacataa cggaggccag gttatccatt
     1261 cagcagatga acgtgttccg tttgcggaag tggtcagcga tggttcggaa gccttcccgt
     1321 tcttgcgcaa tgtgttgccg ggtatcggct gctgcctgta cggcgctgcc tgcacctatg
     1381 ataattcgcc tgacgaggat tttattatcg ataccttacc cggccacgat aataccctgc
     1441 ttattaccgg cctgagtggg cacggtttta aatttgcgtc agttttaggg gaaatagctg
     1501 ccgattttgc gcaagacaaa aaaagcgatt ttgatttgac gccattcagg ctttcccgct
     1561 tccaataatc atcattacgg cctccagaat gaggccgttt ttacaggggt atttaatgcg
     1621 tcggttggtg aactatctca ttaataatat tcgcgagcat ctgatgcttt atctttttct
     1681 ctggggacta ttagcaatag tggacctaat ctacgtgttt tatttttagt ttttccgcac
     1741 tgacataaac tgacaaatat ttaatctccc tttcatttag cgtgaaaaat tgaatgagat
     1801 tcttcctgcc tctacagcac tcactagacc ttccagatgc tgaacccact aaacttatga
     1861 agattctttc attattaatt caaaattatt gaaataaaaa taaatatcat tcaatttgtt
     1921 atttttgtgt tgcaattcat tctccgacac gccattttct ttctctcgca aattcgtttt
     1981 ggatgcactt atgtcattca caaatacccc tgaacgctat ggtgttatat ctgcggcctt
     2041 acattggcta agcgcaatta ttgtctatgg catgtttgct ttaggtctgt ggatggtcac
     2101 gctcagttat tacgatggct ggtaccacca ggcaccggaa ctgcataaaa gcatcggcat
     2161 tttgttaatg atggggctgg ttattcgcgt tctgtggcgt gtcatatctc caccgccggg
     2221 tccgctgccg agttattcgc ccatgactcg tcttgcagca aaagctggac atctcgcatt
     2281 gtacctattg ctgttcgcca tcggcatcag cggctatctg atctcaactg ccgatggtaa
     2341 accgatcagc gtttttggct ggtttgacgt ccccgcgacc ctctccgacg ctggcgcaca
     2401 ggctgacttt gccggtgccc tgcatttttg gctcgcgtgg agcgttgtag tactgtccgt
     2461 tatgcacgga tttatggccc tgaagcatca tttcatcgat aaagacgaca ctctgaagcg
     2521 catgctggga aagtcgtcat ctgactatgg agtataaaaa atgaaaaaaa gcctgcttgg
     2581 tttaaccttc gcttccttga tgttctctgc cggttcagcg gttgccgccg attacaaaat
     2641 tgacaaagaa ggtcagcacg cctttgttaa cttccgcatc cagcatcttg gctatagctg
     2701 gttatatggc acctttaaag atttcgacgg tacttttacc tttgatgaaa aaaatccagc
     2761 tgccgataaa gtgaatgtga caattaacac caccagcgtc gatactaatc acgccgaacg
     2821 cgataaacat cttcgcagtg cggatttcct caataccgca aaatatccac aggcaacatt
     2881 cacctccacc agcgtgaaga aagacggtga cgaactggat attaccggcg atctgacgct
     2941 gaatggtgta accaagcccg tcacactgga agcgaaatta attggtcagg gtgacgaccc
     3001 gtggggcggt aaacgtgcag gctttgaggc cgaaggcaaa attaagctca aagacttcaa
     3061 tatcaagaca gatttaggtc cagcgtctca ggaagtggat ctgattattt cagtggaagg
     3121 cgtacagcag aagtaagagt gaaatgttgc cggatggcac gcatccggca atgatattta
     3181 ttctgttgga tcaggaatgc ccagtgttgt attcagacgt ccacgagact tattaaagat
     3241 cttattgcca ttttcacgtc ctgcccgacg acgtcgctgt tcatctggcg gtaacgcgct
     3301 ttcttcgcag caaatctcac tacaacaacc tttgtatttt tccgcacata ctggacactg
     3361 aataaacagc agatggcagc catcattttt acagttggta tggctgtcgc acggcgcacc
     3421 gcactggtgg cagtgcgcga taatctcatc agatatacgt tcgcccatcc gctcgtcaaa
     3481 aacaaaattt ttgccaataa aacgcaccgg caagccctga tcgcgcgcct tacgggcgta
     3541 ttcaataatt ccgccctcga tatgccagac tttgttgaat ccgttatgtt tcatccaggc
     3601 actggccttt tcacaacgaa tgccgccggt gcagtacatg acgatttttt tatctttatg
     3661 tgcctgcatc atctcaactg cttttggcag ctgctcacgg aaggtatctg ccggtatttc
     3721 cagcgcgttt tcaaagtgcc ccacttcata ctcatagtgg ttacgcatgt cgataaacaa
     3781 tgcatcggga tcgtcaagca tggcgttcac ttccgctgct tgcagatact caccaacatt
     3841 gctggcatca aagtgaggat cgtcaatacc gtcggcaaca atgcgatcgc gtaccttcat
     3901 acgcagtacc cagaaggatt tcccgtcatc atccaacgcg atattcaggc gtaaaccctc
     3961 taaagccggg tcgaaggcat agagctgcgc gcgaaatgtt tcaacattgc tcgcaggtac
     4021 gctgatttgc gcgttaatgc cctcatgcgc cagatacact cgcccaaaaa cattcagcgc
     4081 ggtaaacagt tgatataaag cgtcacgggt caccttagga tcggcaatgt ggaaatactt
     4141 ataaaacgaa atggtggttc gcggttcgct ctcagccaac attttggctt ttaacgcgtc
     4201 gttggaaatg cggttgtgta acactggcat ggtgtacggt tcctgcgaga tgggaaagta
     4261 aaaatccgcg gcatgatata gcaattatcg ataattaaca tccacacatt ttacgctaca
     4321 ttttagcatt aaaaattatt tgttatttac aagcgcggca atttcgccca gtcttcagtc
     4381 acaaattttg gttgcggacg aaaaaatgcg acaatacata caattgcccg tataggttga
     4441 aaaacaggat tgatatgacg aatctaccca agttctccac cgcactgctt catccgcgtt
     4501 attggttaac ctggttgggt attggcgtac tttggttggt cgtgcaattg ccctacccgg
     4561 ttatctaccg cctcggttgt ggattaggaa aactggcgtt acgttttatg aaacgacgcg
     4621 caaaaattgt gcatcgcaac ctggaactgt gcttcccgga aatgagcgaa caagaacgcc
     4681 gtaaaatggt ggtgaagaat ttcgaatccg ttggcatggg cctgatggaa accggcatgg
     4741 cgtggttctg gccggaccgc cgaatcgccc gctggacgga agtgatcggc atggaacaca
     4801 ttcgtgacgt gcaggcgcaa aaacgcggca tcctgttagt tggcatccat tttctgacac
     4861 tggagctggg tgcgcggcag tttggtatgc aggaaccggg gattggcgtt taccgcccga
     4921 acgataatcc actgattgac tggctacaaa cctggggccg tttgcgctca aataaatcga
     4981 tgctcgaccg caaagattta aaaggcatga ttaaagccct gaaaaaaggc gaagtggtct
     5041 ggtacgcacc ggatcatgat tacggcccgc gctcaagcgt tttcgtcccg ttgtttgccg
     5101 ttgagcaggc tgcgaccacg accggaacct ggatgctggc acggatgtcc ggcgcatgtc
     5161 tggtgccctt cgttccacgc cgtaagccag atggcaaagg gtatcagttg attatactgc
     5221 cgccagagtg ttctccgccg ctggatgatg ccgaaactac cgccgcgtgg atgaacaaag
     5281 tggtcgaaaa atgcatcatg atggcactag agcagtatat gtggttacac cgtcgcttta
     5341 aaacacgccc ggaaggcgtt ccttcacgct attaaatctt ccatgctgga tgcttcagaa
     5401 tggcatccgg cattaccaca gaaaatcccc ctgatttagc gataaaagct ctctggattg
     5461 cgccccctgg aagtcgggcg cataattagt gtgcttatct tttcttctta tgttcaccgc
     5521 gcctggcgca ccaacagcgg attgctatgt caccctgtga aaatgacacc cctataaact
     5581 ggaaacgaaa cctgatcgtc gcctggctag gctgttttct taccggtgcc gccttcagcc
     5641 tggtaatgcc cttcttaccc ctctacgttg agcagcttgg cgttaccggc cactccgccc
     5701 tgaatatgtg gtccggtatt gtcttcagca ttacattttt attttcggcc atcgcctcac
     5761 cgttttgggg tggactcgcc gaccgtaaag gccgaaaaat catgctatta cgctctgctc
     5821 tcggcatggg catcgtgatg gtgttgatgg gactggcaca aaatatctgg cagtttttga
     5881 tcctgcgggc gcttcttggt ttacttggcg gatttgtccc caacgctaat gctcttatcg
     5941 ccacacaagt accgcgtaat aaaagcggct gggcgctggg tacgctctcc acaggcggcg
     6001 ttagcggcgc gttgctcggc ccaatggctg gcggtttgct cgccgatagc tacggcttac
     6061 gtccggtatt ctttattacc gccagtgtgc tcatactctg ctttttcgtc accctgtttt
     6121 gcatcagaga aaaattccag ccggtcagca aaaaagagat gctgcatatg cgggaagtgg
     6181 tgacatcact taaaaacccg aaactggtac tcagcctgtt tgtcaccacg ttaatcatcc
     6241 aggtggcgac gggctcaatt gcccccattc tgacgctgta tgtccgcgaa ctggcgggta
     6301 acgtcagtaa cgtcgccttt atcagtggca tgatcgcctc ggtgccaggc gtggcggctc
     6361 tgctaagtgc accacgactc ggcaaacttg gcgatcgaat tggccccgaa aagatcctga
     6421 ttacggcgct gatcttttct gtactgctgt tgatcccaat gtcttacgtt cagacgccat
     6481 tacaacttgg gattttacgt tttttgctcg gtgccgccga tggtgcacta ctccccgccg
     6541 tacagacact attagtttat aactcgagca accagatcgc cgggcgtatc ttcagctata
     6601 accaatcgtt tcgtgatatt ggcaacgtta ccggaccatt gatgggagca gcgatttcag
     6661 cgaactacgg tttcagggcg gtatttctcg tcaccgctgg cgtagtgtta ttcaacgcag
     6721 tctattcatg gaacagtcta cgtcgtcgtc gaatccccca gatatcgaac tgatttttcg
     6781 cctttcatac ttgcaaaagc ggagaatcag ctattctttt ccctgaaacc tcatcaactc
     6841 aaagggagaa tcgtgatgac catgtacgca acgcttgaag aagccattga cgctgcacgc
     6901 gaagaatttc ttgcagacaa ccccggcatc gacgccgaaa acgcgaatgt gcaacagttc
     6961 aatgcccaaa aatacgtttt gcaggacggc gacatcatgt ggcaagttga gttttttgcc
     7021 gacgaagggg aagaaggtga atgtttaccc atgcttagcg gtgaagctgc gcaaagtgtc
     7081 tttgacggcg actatgatga gatagagatc cgccaggagt ggcaagatga gaatacatta
     7141 catgaatggg acgaggggga atttcagctt gagccaccgc tggataccga ggaaggacgc
     7201 actgcagctg atgagtggga tgaacgttaa tcactcatac gggccatgat gtatgtcgat
     7261 cggcagcagt aaagtatcga aaaccaggga gaatggcaga tcaaggatcg tgacgtaacg
     7321 ccaggcggag tcacgcacgt cccattgcac accgggataa tattggttgc catgcccctg
     7381 ccccggtata gtgcgactaa tgatgctgcc acagccactc agcaaggtca ccattatgct
     7441 catcacaatt aaacgcattt gatacctctt ccgtaaaaaa taccggcata acgccggtat
     7501 ttttattggc tcgttgtttt tgccggatgc ggcgtgaacg ctttacccga cctacaaaat
     7561 cttgcaaaat caataaattg caggaacgat gtaggcctga taagcgtagc gcatcaggca
     7621 actacgtttt tattgcgaag ccgcatccgg tttacgtaat gccagtggat tgagctttat
     7681 cccttcgtaa taactcaccc atgaggaata tctctccggg gaattccaga cgcggaaatg
     7741 cagacgggcc atcgtcaccg gatcgcttag cagcaccagg cgacgatcgc gattcagctt
     7801 ctctggcgtc tcgttcagcg cctgttcaac gtggcggtca cgggcgattt ccagcacctt
     7861 gctggcgcgg tgacgcgcgg tcgccattgc ggttgccaga gcgttaaatg acgggttaaa
     7921 caccgcgtgc ataaaaccat catcaaggga gcgttgacga ttcatctcaa ggaaccgatc
     7981 ggtatcaacc agcacctgcg gcggtgaata ctcttccggg atcaggaaca gtttccagcg
     8041 tttggttcgc agaccaacgg tggcacggct ggaaatcacc gaaacaaacg gtgacaggat
     8101 caacgagaag acaatcggtg ccagccagaa cagaaaacgc agatccaacc acgccatccc
     8161 aacagcccac actaaaccca acagcagctg tgagccgtgg cgtttgaatg cttcgcccca
     8221 ggaagtggag tcatcatcac gctgcggtga attccacacc acttcccagc caaggaacgc
     8281 gctgacgacg aagactgtat ggaacaacat gcgtaccgga gccagcaaca cggaaaacag
     8341 cacttccagc agcaacgata atgtaacgcg ccagaagccg ccgtattctt tcgttccttt
     8401 gcaccagata agcaaaatgc tcaatagctt cggcaggaac aaaagcacca tggtcgaagc
     8461 aaaaagtgca atcgccagct caggacgcca ctgcggccat accgggaaca actgccgtgg
     8521 ttgcaggaag tattgcggtt cggtcaacgc atgtactacc tgcaatgcag tagagagcgc
     8581 gaggaacata aaccacagcg gagcggagag ataagacatc acgcccgtca ggaacaccgc
     8641 acggtgaacc gggtgcatac ccttcaccag gaacagacgg aagttcatca ggttaccgtg
     8701 gcaccagcgg cggtcacgtt ttagctcatc aagcaagtta ggcggcaatt cttcataaga
     8761 acccgggaga tcgtaagcaa tccagacccc ccaacctgca cggcgcatca acgccgcttc
     8821 cacgaagtca tgtgacagga ttgaaccggc aaaagaacct tcgcccggca gcggagccag
     8881 tgcacagtgc tcgataaacg gtttcacgcg gataatcgcg ttatgccccc agtagtgcga
     8941 ctcgccaagt tgccagaagt gcaaaccggc tgtaaacagt ggcccataca cgcgggtcgc
     9001 gaactgctga cagcgcgcat acagcgtatc cataccggac gctttcggcg acgactgaat
     9061 gatcccggcg ttcgggttgg cttccatcag gcgcaccagg ccgcacaaac aatcaccggt
     9121 cattaccgag tcagcatcca gcaccaccat gtagctgtac tggctgcccc agcgacggca
     9181 gaagtcatcg atattaccgc ttttacgctt cacgcgacgg cggcggcggc gatagaaaat
     9241 ctgaccttcg ccaccgactt cagcgataag ctccatccag gctttttgct ctgcgacgca
     9301 gatatccggg ttataactgt cactaagaat gtagacatca aaatgtttgg cattcccggt
     9361 ggcttttact gattcccacg ttgcacgcaa gccagcaaaa acacggttca cgtcttcgtt
     9421 acagataggc atgatcaacg ccgtgcgatg ctccgggttt aatggttcat cgccaactgt
     9481 tgacgcagat atactgtatt tatcgcgacc aataagcagt tgcaggaagc ccatcaacgc
     9541 cgtccagaat ccggcggaca cccaacagaa cagtaccgca aagaggatca ggataccggt
     9601 ttgcagcata taaggcagaa gctgcataaa ggaaacccac acatcctgac caaccatatc
     9661 cataggatta atcagcgccc acccctgata aggaagaatg gtcttcatat accaggtcgc
     9721 gacaacagtt tgcgcgagcg tcaggatcaa cagaatgtaa cggcggatgg taccgacggt
     9781 acgccacttt tgctcactct cctgctcttc tttggtcaaa cgagccagat agcgcggcgt
     9841 cacatcgcgt ccacgcaggc gatcccagaa acggcctacc gggttggtac gccacgggtc
     9901 gggaaacatc gaggagcgtt ttacttctgg catcgccttt agctgatcgc gcccttcgtc
     9961 gtctttaatt aactgtccat cagcaagtga atctggccag gcttgttcca gacgcgcctt
    10021 taccgagcct tgcggggagt catcctcccg cgcccaggtg cggtgttcgg catccagcgc
    10081 ctgatgaacg gcgcggatat cagtcttcgg caatgccgct ttctcgcttg cggcgatggg
    10141 cattgcgtca atgtactcgg ttgtcttatt cattggcagg taactggtag ctccaggttt
    10201 cactcaacgt ctgatcggca ttcaccagcg cagcacgcat ttcagtggtt ttcttggcat
    10261 ctttcacttt cacacgcatc accagacgcc aacctttggt aaccgggtta tagcgcaccg
    10321 tgctttcaac tatctcgcca ttatcaccaa tgctggtttg cgctgtgacc ggggtatcct
    10381 ctggcagttt tttcatctca gcgccggtaa aatcgaccac aaaggcgata gtaccgtcag
    10441 gctggcgaat caggttcgac tgcttcacat cccccgttga acgacgcgtt tgttgcaccc
    10501 atgcgttatc tggcgcatgc agtttgtctt catcacggct gaaggtgatg gtgtatttaa
    10561 agttcatctc tttacccggc tccggcagct gatccggcgt ccagtaagcg acgatgttat
    10621 cgttggtttc atcgttagtt ggaatttcca ccagctcaac gctgcctttg ccccactccc
    10681 ctttcggagt cacccatgcg cttggacgaa gatcgtaacg atcatcgaga tcttcaaagc
    10741 gggagaaatc acgaccacgc tgcaatagac cgaagccttg cgggttttcc atcgagaagc
    10801 tgctgaccgc taaatgtttc gggttattca acggacgcca gatccattca ccattaccag
    10861 catggataga cagaccgtta gagtcatgca actccggacg atagttattt gcaggcgacg
    10921 gttggttcgg cccaaacagg aacatactgg ttaacggtgc aacccccagt ttgccgactt
    10981 tatcgcgcag atagattttc gactgcacat ccacaaccgt gtcacgtcct ggcataacta
    11041 cgaatttgta agcacctgtc gcgcgcggcg agtcaagcaa tgcataaatg gttaaacgtt
    11101 tatcagtcgg ttttggacgc tcgatccaga actctttgaa gcgtggaaat tcttcacccg
    11161 atggcaaggc ggtatcaatt gccaggccgc gtgcagaaag gccataaacc tgacctgcac
    11221 caatcacgcg gaaatagctg gccccgagca tgctgacgat ttcatcgttt ttatctttgc
    11281 tgttgatcgg gtaaagcact ttgaaaccgg caaaaccaag gtcttttacc gtgtctttgt
    11341 catgctgaac atcgccgaaa gtgaaataat ctgggctgta tttgattcgt ttgactgcgg
    11401 tggcagtcac ttcatttatt ttgaccgggg tatcgaagta cataccctga tggtagaact
    11461 cgagtttgaa tggggtcttc agattgttcc agtacgcttt gtcatgatta aactggatct
    11521 gctgatagtc cgcgtatttc atatcgcgga aaacggaggg caagttgctt ttgggcgcct
    11581 catagccttt cccggctaag gattgagctt gctttgcgac atcatcaata ctgaaagccc
    11641 agcttgaaga tgtatacagg gttaacatta ctgcagcact caaccaacgc attttcatca
    11701 tttgtagttt atgtttcata attagtaagc acttccccct ttgtgtgctt atatcgatcc
    11761 gatctatttt aatggataac ctgccattcc gacaacagaa tcccggtatc gttcagcaca
    11821 ctggctcatg gattaagcac ctgattgcaa ccgttttcac tttccgtgct tctcctaaag
    11881 acagaagcat cgacttcgtg tagggtttgc cagcaaattt atttatcaac cgtcttacag
    11941 acgcttccat tctggttagt ggtagcgatg atgctatgca taaaaacctt gaagcctgcg
    12001 cctggcgcgg tcaggtcggt gtcgctatgg caacaagcct aaaggcataa cccgacaggg
    12061 ataagacgaa aaatccgaga ttagttaacc atatatgaat ccagtacccg cgcaacgtga
    12121 atatttcctc gactccatcc gcgcctggct gatgttgtta gggatccctt ttcatatttc
    12181 tttaatctat tcgagccata catggcatgt gaatagcgcc gaaccgtcat tgtggctgac
    12241 cctttttaat gacttcatcc actcgttccg catgcaggtg tttttcgtta tatccgggta
    12301 cttttcctac atgctttttt tacgctatcc cttgaaaaaa tggtggaaag tacgtgtcga
    12361 acgtgtaggg atcccgatgt taacagccat ccccttactg acattgccgc aatttattat
    12421 gctgcaatac gtcaaaggga aagcggaaag ttggcctggg ctgtcattgt atgacaaata
    12481 taatacgttg gcctgggaat taatatcaca cctgtggttt ttactggtgt tagtggtcat
    12541 gacgacgctg tgcgtatgga tatttaaacg ca
//
LOCUS       DADSYM010000138        12549 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2415
            SAMN08039579-rid11723173.denovo.139, whole genome shotgun sequence.
ACCESSION   DADSYM010000138 DADSYM010000000
VERSION     DADSYM010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039579
            Sequence Read Archive: SRR6362533
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12549)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12549)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980162.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 03:39:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,484
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 4,347
            CDSs (with protein)               :: 4,347
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 43 of 85
            Pseudo Genes (incomplete)         :: 37 of 85
            Pseudo Genes (internal stop)      :: 19 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12549
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039579-rid11723173.denovo.139"
                     /strain="LGE2415"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8302 N 95.9628 W"
                     /collection_date="2015-08-24"
     gene            complement(143..796)
                     /gene="ribB"
                     /locus_tag="J1Y83_003501"
     CDS             complement(143..796)
                     /gene="ribB"
                     /locus_tag="J1Y83_003501"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001077009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxy-2-butanone-4-phosphate synthase"
                     /protein_id="HBA7631616.1"
                     /translation="MNQTLLSSFGTPFERVENALAALREGRGVMVLDDEDRENEGDMI
                     FPAETMTVEQMALTIRHGSGIVCLCITDDRRKQLDLPMMVENNTSAYGTGFTVTIEAA
                     EGVTTGVSAADRITTVRAAIADGAKPSDLNRPGHVFPLRAQAGGVLTRGGHTEATIDL
                     MTLAGFKPAGVLCELTNDDGTMARAPECIEFANKHNMALVTIEDLVAYRQAHERKAS"
     regulatory      complement(900..1048)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            1170..1469
                     /locus_tag="J1Y83_003502"
     CDS             1170..1469
                     /locus_tag="J1Y83_003502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311957.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory factor UbiK family protein"
                     /protein_id="HBA7631617.1"
                     /translation="MIDPKKIEQIARQVHESMPKGIREFGEDVEKKIRQTLQAQLTRL
                     DLVSREEFDVQTQVLLRTREKLALLEQRISELEARNNPVAELQSPPAIPPVDKAE"
     gene            complement(1504..1704)
                     /gene="glgS"
                     /locus_tag="J1Y83_003503"
     CDS             complement(1504..1704)
                     /gene="glgS"
                     /locus_tag="J1Y83_003503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell surface composition regulator GlgS"
                     /protein_id="HBA7631618.1"
                     /translation="MDHSLNSLNNFDFLARSFARMHAEGRPVDILAVTGNMDEEHRTW
                     FCARYAWYCQQMMQTRELELEH"
     gene            1974..2603
                     /locus_tag="J1Y83_003504"
     CDS             1974..2603
                     /locus_tag="J1Y83_003504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqiJ family protein"
                     /protein_id="HBA7631619.1"
                     /translation="MILFADYNTPYLFAISFVLLIGLLEIFALICGHMLSGALDAHLD
                     HYDSITTAHISQALHYLNIGRLPALVVLCLLAGFFGLIGILLQHACVTLWQSPLPNLF
                     VVPVSLLFTIIAVHYTGKVVAPWIPRDHSSAITEEEYVGSMALITGHQATSGNPCEGK
                     LTDQFGQIHYLLLEPEEGKTFTKGDKVLIICRLSATRYLAENNPWPQIL"
     gene            2630..4291
                     /locus_tag="J1Y83_003505"
     CDS             2630..4291
                     /locus_tag="J1Y83_003505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin family protein"
                     /protein_id="HBA7631620.1"
                     /translation="MDDIVNSVPSWMFTAIIAVCILFIIGIIFARLYRRASAEQAFVR
                     TGLSGQKVVMSGGAIVMPIFHEIIPINMNTLKLEVSRSTIDSLITKDRMRVDVVVAFF
                     VRVKPSVEGIATAAQTLGQRTLSPEDLRMLVEDKFVDALRATAAQMTMHELQDTRENF
                     VQGVQNTVAEDLSKNGLELESVSLTNFNQTSKEHFNPNNAFDAEGLTKLTQETERRRR
                     ERNEVEQDVEVAVREKNRDALSRKLEIEQQEAFMTLEQEQQVKTRTAEQNAKIAAFEA
                     ERRREAEQTRILAERQIQETEIDREQAVRSRKVEAEREVRIKEIEQQQVTEIANQTKS
                     IAIAAKSEQQSQAEARANLALAEAVSAQQNVETTRQTAEADRAKQVALIAAAQDAETK
                     AVELTVRAKAEKEAAEMQAAAIVELAEATRKKGLAEAEAQRALNDAINVLSDEQTSLK
                     FKLALLQALPAVIEKSVEPMKSIDGIKIIQVDGLNRGGATGDANTGNVGGGNLAEQAL
                     SAALSYRTQAPLIDSLLNEIGVSGGSLAALTSSLSSTTPVEEKAE"
     gene            4531..4590
                     /locus_tag="J1Y83_003506"
     CDS             4531..4590
                     /locus_tag="J1Y83_003506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HBA7631621.1"
                     /translation="MMKLVIILIVLLLVSFAAY"
     gene            complement(4710..6143)
                     /gene="hldE"
                     /locus_tag="J1Y83_003507"
     CDS             complement(4710..6143)
                     /gene="hldE"
                     /locus_tag="J1Y83_003507"
                     /EC_number="2.7.1.167"
                     /EC_number="2.7.7.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     D-glycero-beta-D-manno-heptose-7-phosphate
                     kinase/D-glycero-beta-D-manno-heptose 1-phosphate
                     adenylyltransferase HldE"
                     /protein_id="HBA7631622.1"
                     /translation="MKVTLPEFERAGVMVVGDVMLDRYWYGPTSRISPEAPVPVVKVN
                     TIEERPGGAANVAMNIASLGANARLVGLTGIDDAARALSKSLADVNVKCDFVSVPTHP
                     TITKLRVLSRNQQLIRLDFEEGFEGVDPQPLHERINQALSSIGALVLSDYAKGALASV
                     QQMIQLARKAGVPVLIDPKGTDFERYRGATLLTPNLSEFEAVVGKCKTEEEIVERGMK
                     LIADYELSALLVTRSEQGMSLLQPGKAPLHMPTQAQEVYDVTGAGDTVIGVLAATLAA
                     GNSLEEACFFANAAAGVVVGKLGTSTVSPIELENAVRGRADTGFGVMTEEELKLAVAA
                     ARKRGEKVVMTNGVFDILHAGHVSYLANARKLGDRLIVAVNSDASTKRLKGDSRPVNP
                     LEQRMIVLGALEAVDWVVSFEEDTPQRLIAGILPDLLVKGGDYKPEEIAGSKEVWANG
                     GEVLVLNFEDGCSTTNIIKKIQQDKKG"
     gene            complement(6191..9031)
                     /gene="glnE"
                     /locus_tag="J1Y83_003508"
     CDS             complement(6191..9031)
                     /gene="glnE"
                     /locus_tag="J1Y83_003508"
                     /EC_number="2.7.7.42"
                     /EC_number="2.7.7.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional [glutamate--ammonia
                     ligase]-adenylyl-L-tyrosine
                     phosphorylase/[glutamate--ammonia-ligase]
                     adenylyltransferase"
                     /protein_id="HBA7631623.1"
                     /translation="MKPLSSSLQQYWQTVVERLPEPLAEESLSAQAKSVLTFSDFVQD
                     SVIAHPEWLTELESQPPQADEWQHYAAWLQEALSNVSDEAGLMRELRLFRRRIMVRIA
                     WAQTLALVTEESILQQLSHLAETLIVAARDWLYDACCREWGTPCNAQGEAQPLLILGM
                     GKLGGGELNFSSDIDLIFAWPEHGCTQGGRRELDNAQFFTRMGQRLIKVLDQPTQDGF
                     VYRVDMRLRPFGESGPLVLSFAALEDYYQEQGRDWERYAMVKARIMGDSDGVYANELR
                     AMLRPFVFRRYIDFSVIQSLRNMKGMIAREVRRRGLTDNIKLGAGGIREIEFIVQVFQ
                     LIRGGREPSLQSRALLPTLSAIAALHLLSENDAEQLRVAYLFLRRLENLLQSINDEQT
                     QTLPSDELTRARLAWAMDFADWPQLTGVLTAHMANVRRVFNELIGDDESETQEESLSE
                     QWRELWQDALQEDDTTPVLAHLSEDDRKQVLMLIADFRKELDKRTIGPRGRQVLDHLM
                     PHLLSDVCAREDAAVTLSRITALLVGIVTRTTYLELLSEFPAALKHLISLCAASPMIA
                     SQLARYPLLLDELLDPNTLYQPTATDAYRDELRQYLLRVPEDDEEQQLEALRQFKQAQ
                     LLRIAAADIAGTLPVMKVSDHLTWLAEAMIDAVVQQAWVQMVARYGKPNHLNDREGRG
                     FAVVGYGKLGGWELGYSSDLDLIFLHDCPMDTMTDGEREIDGRQFYLRLAQRIMHLFS
                     TRTSSGILYEVDARLRPSGAAGMLVTSAEAFADYQKNEAWTWEHQALVRARVVYGDPQ
                     LTAHFDAVRREIMTLPREGKTLQTEVREMREKMRAHLGNKHRDRFDIKADEGGITDIE
                     FITQYLVLRYAHEKPKLTRWSDNVRILELLAQNDIMEEQEAMALTRAYTTLRDELHHL
                     ALQELPGHVPEDCFTAERELVRASWQKWLVEE"
     gene            complement(9054..10355)
                     /locus_tag="J1Y83_003509"
     CDS             complement(9054..10355)
                     /locus_tag="J1Y83_003509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic triphosphatase"
                     /protein_id="HBA7631624.1"
                     /translation="MAQEIELKFIVNHSAVEALRDHLNTLDGEHHDPVQLLNIYYETP
                     DNWLRGHDMGLRIRGENGRYEMTMKVAGRVTGGLHQRPEYNVALSEPTLDLAQLPTEV
                     WPNGELPTDLASRVQPLFSTDFYREKWLVEVDGSRIEIALDQGEVKAGEFAEPICELE
                     LELLSGDMRAVLKLANQLVSQTGLRQGSLSKAARGYHLAQGNPAREIKPTTILHVAAK
                     ADVEQGLEAAFELALAQWQYHEELWVRGNDAAKEQVLAAIGLVRHTLMLFGGIVPRKA
                     STHLRDLLTQCEATIASAVSAVTAAYSTETAMAKLALTEWLVSKAWQPFLDAKAQSKI
                     SDSFKRFADIHLSRHAAELKSVFCQPLGDRYRDQLPRLTRDIDSILLLAGYYDPVVAQ
                     DWLENWQGLRHAIATGQRIEIEHFRNEANNQEPFWLHSGKR"
     gene            10597..11217
                     /locus_tag="J1Y83_003510"
     CDS             10597..11217
                     /locus_tag="J1Y83_003510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HBA7631625.1"
                     /translation="MPKLRLIGLTLLALSATAVSHAEETRYVSDELNTWVRSGPGDHY
                     RLVGTVNAGEEVTLLQTDANTNYAQVKDSSGRTAWIPLKQLSTEPSLRSRVPDLENQV
                     KTLTDKLTNIDNTWNQRTAEMQQKVAQSDSVINGLKEENQKLKNELIVAQKKVDAASV
                     QLDDKQRTIIMQWFMYGGGVLGLGLLLGLVLPHLIPSRKRKDRWMN"
     gene            11281..12519
                     /gene="cca"
                     /locus_tag="J1Y83_003511"
     CDS             11281..12519
                     /gene="cca"
                     /locus_tag="J1Y83_003511"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused tRNA nucleotidyltransferase/2',3'-cyclic
                     phosphodiesterase/2' nucleotidase/phosphatase Cca"
                     /protein_id="HBA7631626.1"
                     /translation="MKIYLVGGAVRDALLGLPVKDRDWVVVGSTSQEMLDAGYQQVGR
                     DFPVFLHPQTHEEYALARTERKSGSGYTGFTCYAAPDVTLEDDLKRRDLTINALAQDD
                     NGEIIDPYNGLSDLQNRLLRHVSPAFGEDPLRVLRVARFAARYAHLSFRIADETLALM
                     REMTHAGELEHLTPERVWKETENALTTRNPQVFFQVLRDCGALRVLFPEIDALFGVPA
                     PARWHPEIDTGIHTLMTLSMAAMLSPQVDVRFATLCHDLGKGLTPPELWPRHHGHGPA
                     GVKLVEQLCQRLRVPNEIRDLARLVAEFHDLIHTFPMLNPKTIVKLFDSIDAWRKPQR
                     VEQLALTSEADVRGRTGFESADYPQGRWLREAWEVAQSVPTKAVVEAGFKGVEIREEL
                     TRRRIAAVASWKEQRCPKPE"
BASE COUNT         3096 a         3429 c         3107 g         2917 t
ORIGIN      
        1 tgaaaaagaa tgcctgatat taatgaaatt ttctcataga aagagaagcg gaattaacat
       61 cttgcatagc agggaaaaat aaaaaccgaa gcctgctaca acttcggttt cttgattaag
      121 gcagtaaatt aagcagcggt tttcagctgg ctttacgctc atgtgcctga cggtatgcca
      181 ccaggtcttc aatagtcacg agcgccatat tgtgtttatt ggcaaactca atgcactctg
      241 gtgcacgcgc catcgtgcca tcgtcattag tcagctcaca cagcacacca gctggtttaa
      301 agcctgccag cgtcatcaga tcgatagtcg cttcagtatg accgccacgc gtcagtacac
      361 cacctgcctg cgcgcgaagt gggaaaacgt ggcccggacg attcagatct gaaggttttg
      421 cgccatcggc aatcgctgcg cgaacggtcg taatacggtc agcggcagaa acaccggtag
      481 tcacaccttc agctgcttca atggtcacgg taaaaccggt gccataggcg ctggtgttat
      541 tttctaccat cattggcaga tcgagttgtt tacggcgatc gtcagtaatg cacaggcaaa
      601 caataccact cccgtggcga atggtcagcg ccatctgctc aacggtcatg gtttctgccg
      661 ggaagatcat atcgccttcg ttttcacggt cttcatcatc aagcaccatc acaccgcgtc
      721 cttcacgcag cgcagccagt gcattttcaa cacgttcgaa aggcgtacca aaagaggaaa
      781 gtagcgtctg attcatggta aaaaaacctc actaaaatta tggttaccag aatcagggca
      841 gtcttaggag tggcggcata tagccaaaat aacgtgagcg ggtccatgcc cgacagaatc
      901 gttactctct cccatccgga ctctaaccgt cggccccgga attacaccgg atctgctgtc
      961 ctttgagttc gcacccaaag cgctcgcggg ctttcaactg agttgattta ccgccggtgg
     1021 ggaatttcgc cccgccctga gaataagcgg attcactata acgctaatga ttagcgccag
     1081 caacgcatag cttcacataa ttctggttta tgacttaccc ttatcgcact acaatggcac
     1141 tcaacaccta tcagtacagg gaaactacaa tgattgaccc gaaaaaaatt gagcaaatcg
     1201 ctcgccaggt tcacgaatca atgcctaaag gaatcaggga gttcggggaa gatgtggaga
     1261 aaaaaatccg ccaaacccta caagcgcagc tgacgcgtct cgatctggta agccgcgaag
     1321 aattcgacgt tcagacgcaa gtcctgttac ggactcgtga aaaactggcg ttactagagc
     1381 agcgcatcag cgaactggaa gcgcgcaata atccggtagc agaacttcaa tctcctccgg
     1441 ctatcccgcc agttgataaa gcagaataaa gcaccacaaa aacgggccgg acggcccgta
     1501 atatcagtgt tctaattcaa gttctctggt ttgcatcatc tgttggcaat accaggcata
     1561 acgtgcgcaa aaccaggttc tatgttcttc atccatgtta ccagtaacgg ccagaatatc
     1621 gaccgggcga ccttctgcgt gcattctggc aaaactacgc gcgagaaaat cgaaattatt
     1681 taaagaatta agactatgat ccataagcac atcctccggt tattgcatac cttttaaggc
     1741 catatgcttt ttctgatttc agtattcccc aatcagtgta gtgcctttaa acataacgtg
     1801 cgtaaatata ttgtccccga tcacactttt tagtgaaaaa taaaaaaacc atattaaaag
     1861 actttctctt ccagaacctc tctaacacgt gttattatta acaaagataa atgtaagcaa
     1921 ggaaactatt ccaaagtaaa tattgcgtca ctaaatggac attggagtga catatgattt
     1981 tattcgccga ctataacacc ccttatcttt tcgctatttc ctttgtactc ttgatcggcc
     2041 ttttagaaat ttttgcactt atctgcggtc acatgctctc cggtgctctt gatgcacatc
     2101 ttgaccatta tgattccata accacagctc atattagcca ggcacttcat tatctcaata
     2161 ttggaagatt accagctctc gtcgtcctct gcttactcgc tggttttttt ggtcttattg
     2221 gcatcctgtt gcaacatgcc tgcgttacgc tctggcaatc accactcccc aacctgttcg
     2281 tggttcctgt cagtttactg tttacaatta ttgcagtgca ttataccggt aaagtcgttg
     2341 caccctggat tccacgcgac catagttcag ctattacaga agaagaatat gttggtagca
     2401 tggcattaat taccggccat caagccactt caggtaaccc ctgcgaagga aaactcaccg
     2461 atcaatttgg ccaaatacat tatttgctac tggagcctga agaaggaaaa actttcacta
     2521 aaggagacaa agtgttaatt atttgccgac tttctgcaac gcgatatttg gcggaaaata
     2581 atccctggcc ccagatattg tgatttatta atcaaaagga aatttaataa tggatgatat
     2641 cgttaattct gtgccctcct ggatgtttac cgcgattatc gccgtatgca ttctgtttat
     2701 tattggaatt attttcgcca ggctctatcg tcgcgcttcg gcagagcaag catttgttcg
     2761 tactggttta agtgggcaaa aagtggtgat gagcggtggc gcaatcgtga tgccgatctt
     2821 tcatgaaata attcccatca atatgaatac cctgaagctg gaagtcagcc gttctactat
     2881 tgatagcctg attacgaaag atcgtatgcg tgttgatgtt gtagtggctt tctttgtgcg
     2941 ggtaaaaccg tcagtagaag ggattgccac cgccgcccag acgctggggc aacgcaccct
     3001 gtcgcctgaa gacttacgta tgctggtgga agataaattt gtcgatgccc tccgtgcaac
     3061 agcggcacaa atgaccatgc atgagttaca ggatacccgc gagaacttcg tgcagggagt
     3121 acaaaatacc gtagctgaag atctgtcgaa aaatggcctg gaactggaaa gcgtttcact
     3181 taccaacttt aaccagacct cgaaagaaca tttcaatccg aacaatgcct ttgacgccga
     3241 aggtttaacc aaactgactc aggagacgga gcgccgtcgt cgcgaacgta acgaagttga
     3301 acaggatgtg gaagtcgcag tgcgtgagaa aaaccgtgat gcactttcgc gcaagttgga
     3361 gattgaacaa caagaagcgt ttatgacgct tgagcaggag cagcaggtta aaacccgtac
     3421 cgctgagcag aatgcgaaaa ttgcggcttt tgaagctgaa cgtcgtcgtg aagcagagca
     3481 gacgcgaatt ctggctgaac gacagattca ggaaacggaa atcgaccgcg aacaggccgt
     3541 ccgctcaaga aaggttgagg ccgaacgtga agttcgcatt aaagagatcg aacagcagca
     3601 ggtcaccgaa atcgctaacc agacgaaatc gatcgctatt gccgccaaat cggaacaaca
     3661 gtcccaggca gaagcgcgtg ctaaccttgc actcgcagaa gcagtaagcg cccaacaaaa
     3721 cgtagaaacc actcgccaga ctgccgaagc cgatcgtgct aaacaagtgg cgttgatcgc
     3781 cgcagcgcag gatgcagaaa ccaaagcggt tgaactgacc gtgcgggcga aagcagaaaa
     3841 agaagccgca gaaatgcagg cggcggctat cgttgagtta gccgaagcta cacgtaaaaa
     3901 gggtctggcg gaagcagaag cacaacgtgc gctgaacgat gctatcaacg tactttctga
     3961 tgaacaaacc agccttaaat tcaaactggc attattgcag gcgctgcctg cggtaattga
     4021 gaaatccgtg gaaccaatga agtctatcga cggcatcaaa attattcagg tcgatgggct
     4081 gaatcgtggt ggcgctacgg gtgatgcaaa cacgggtaat gtggggggcg gaaacctggc
     4141 agaacaagcg ttatcagccg cactctctta ccgtacacag gcaccgctga ttgactcctt
     4201 gctcaatgaa attggcgttt caggcggctc actggcggca ttgacttcat ccttaagctc
     4261 aacaactccc gtcgaagaaa aagcagagta aaccctcttc tatgaataat ccctggccca
     4321 aaaagccggg gattttttat atctgcgttc cgctaaaagg tgcaaatgat caagctgttg
     4381 cagcattttg cgtgactgct cggggaaggc aaaactgcct ctgggaaagc gttgcgcggg
     4441 gtccggcgct catcaacaat cggggagtca gcaaggggct gaaacgggaa agcccctccc
     4501 gaggaagggg ccttgtataa ggaaagggtt atgatgaagc tcgtcatcat actgattgtg
     4561 ttgttactcg taagtttcgc agcttattaa cagccaatca gaggggggag aaatcctccc
     4621 tcacccttgt tcctttactt taggttgaaa aaaaatcagc gtcaataggc ccgcctgcta
     4681 caaagcgaga tctgtgaacc gctttccggt tagccttttt tatcctgttg gatcttcttg
     4741 atgatgttgg tcgtcgagca accgtcttca aagttgagca ccagcacttc gccaccgttg
     4801 gcccagactt ctttactccc ggcaatctct tctggtttat agtcgccgcc tttcaccagt
     4861 agatctggca agatcccggc gatcaagcgc tgcggcgtgt cctcttcaaa cgacaccacc
     4921 cagtcgaccg cttccagtgc gcccagcaca atcatacgct gttcgagcgg gttaacgggg
     4981 cgggaatccc ctttcagccg tttggtggag gcatcgctgt tcacagcgac aatcaaacgg
     5041 tcacccagtt tacgggcatt tgccagataa gagacgtgcc cagcgtgcag gatgtcaaag
     5101 acaccgttgg tcatcaccac tttttcaccg cgtttacgcg ctgccgctac agcaagcttc
     5161 agttcctctt cggtcatcac accaaagccg gtatctgcac gtccacgtac tgcattttcc
     5221 agctcgatcg gcgataccgt ggatgtcccc agtttgccga ccaccacgcc tgccgccgca
     5281 ttggcaaaga agcaggcttc ttccagcgaa ttacccgctg ccagcgttgc cgccaggaca
     5341 ccaatcaccg tgtcgcccgc accggtaacg tcatacactt cctgcgcctg ggttggcata
     5401 tgcagcggcg ctttacccgg ttgcagcagc gacataccct gttcggaacg ggtcactaac
     5461 agagccgaga gttcgtaatc ggcaatcagt ttcatgccgc gctcaacaat ctcttcttcg
     5521 gtcttacatt taccgacaac agcttcaaat tccgagagat tcggcgttaa cagcgtagcg
     5581 ccgcggtagc gctcaaaatc ggtacctttt ggatcaatca gcaccggaac acccgcttta
     5641 cgcgccagtt ggatcatctg ctgtacgctt gccagcgcac ctttggcgta gtcagaaagc
     5701 accagcgcgc caatcgaact cagcgcctga ttaatccgtt cgtgcagcgg ttgcggatca
     5761 acaccttcga aaccttcttc aaaatccaga cggatcagct gttggttgcg ggaaagtacc
     5821 cgtaatttgg tgatggtcgg atgcgtcggt acagaaacga agtcgcattt gacgttgacg
     5881 tcagccagag atttactcag cgcgcgcgct gcatcgtcaa tgcccgtcaa cccgaccagg
     5941 cgtgcattag caccgagaga agcgatattc atcgccacgt tagccgcgcc gcccggacgt
     6001 tcttcgatgg tattcacttt aaccacgggc accggcgctt ccggcgagat acggctggtg
     6061 ggaccgtacc agtaacgatc cagcatcaca tcaccaacca ccatcactcc tgcacgttca
     6121 aactctggca gcgttacttt cattcctgtc tcctgagaga ttcaaaattt gcgcgcgata
     6181 ataccatact tcattcttcc accagccact tctgccagct tgcccgcacc agttcacgct
     6241 ctgcggtgaa gcaatcctcc ggcacatggc ccggcaattc ctgtaatgcc aggtgatgaa
     6301 gttcatcgcg cagcgtcgtg taagcacggg tcagcgccat cgcttcctgt tcttccataa
     6361 tgtcgttttg cgccagcagt tccagaatac gcacgttgtc tgaccagcgc gttaacttcg
     6421 gtttttcatg agcgtagcgc aacaccagat attgggtaat aaattcgata tcggtaattc
     6481 cgccttcatc ggctttgata tcaaagcgat cgcgatgttt attgccgaga tgagcgcgca
     6541 ttttctcgcg catttcccgc acttccgttt gcagagtttt accttcacgc ggcagcgtca
     6601 taatctcgcg acgcaccgcg tcaaagtgcg cggtgagctg cggatcgccg tacactacac
     6661 gcgcacgcac cagcgcctga tgttcccacg tccaggcctc gtttttctga taatcggcaa
     6721 acgcttctgc ggatgtcacc agcattcccg ccgccccgga cggacgcaac cgtgcatcca
     6781 cctcatacaa aatgccggaa gaggttcgcg tactgaacag atgcataatg cgttgcgcca
     6841 gacgcagata aaactgtcgc ccgtcgattt cccgctcacc gtcagtcatc gtatccattg
     6901 ggcaatcatg gaggaagata aggtcaagat cggaactgta acctaactcc cagccgccca
     6961 gcttgccgta gccgaccacc gcaaaaccac ggccttcgcg atcgttgagg tgatttggct
     7021 taccgtagcg ggccaccatt tgaacccacg cctgctgaac aacggcatca atcatggctt
     7081 ccgccagcca ggttaagtga tcgctcactt tcattaccgg cagcgtaccg gcgatatccg
     7141 ctgccgcaat gcgtaacagc tgcgcctgtt tgaactgacg cagcgcctca agctgttgtt
     7201 cttcgtcatc ttccggcacg cgcagcaaat actggcgcag ttcatcacgg taggcatccg
     7261 tcgccgttgg ttggtagagg gtatttggat cgagcaattc atccagcaat aatggataac
     7321 gcgctagctg gctggcaatc atcggcgatg cagcacacag agaaatcaaa tgtttaagcg
     7381 ccgcagggaa ttcactaagc agctcaagat aagtggtgcg agtaacaatc cccaccagca
     7441 aggcggtaat gcgcgacagc gtgacagcag cgtcttcacg cgcacagaca tcacttagca
     7501 gatgtggcat cagatggtcg agcacctgac gtcctcgcgg cccgatggtg cgcttatcca
     7561 gctctttgcg aaaatcagca atcagcatta gcacctgttt gcgatcatcc tcgctaagat
     7621 gcgccagcac tggcgtggtg tcatcttcct gcaacgcatc ctgccacagc tcacgccact
     7681 gttccgacag cgactcttcc tgagtttcac tttcgtcgtc gccaatcaat tcattaaaca
     7741 cccggcgcac attggccata tgtgcggtca gcaccccggt caattgcggc cagtcagcaa
     7801 agtccatcgc ccacgccagc cgcgcacggg taagctcatc agaaggaagc gtctgggttt
     7861 gttcgtcgtt aatgctttgc agcaggtttt ccagacgccg caggaacaga tacgccactc
     7921 gcaattgttc agcatcgttt tcagaaagca gatgcagcgc ggcaatggcg ctgagcgttg
     7981 gcagtaaagc gcgcgattgc agcgacggtt cgcgcccgcc gcgaatgagc tggaacacct
     8041 ggacgataaa ttcaatttcg cgaatgccgc ctgcgccaag tttgatgttg tcggtcaaac
     8101 cgcgtcgacg cacttcacgg gcaatcattc ctttcatgtt gcgcagcgac tgaatcacgc
     8161 tgaaatcgat gtaacgacgg aaaacaaacg gacgtagcat cgcacgcaac tcgttagcat
     8221 agacgccgtc gctatccccc ataatccgcg ccttgaccat cgcgtaacgc tcccagtcgc
     8281 gcccctgctc ctggtaataa tcttccagcg ccgcaaaact cagcaccagc gggccacttt
     8341 caccaaacgg acgcagccgc atatccacgc gatagacgaa accatcctgc gttggttgat
     8401 ccagcacttt aatcagccgc tgccccatgc gggtaaaaaa ctgcgcgtta tccagttccc
     8461 ggcgtccgcc ctgggtacaa ccatgttccg gccaggcaaa aatcagatcg atatctgagg
     8521 agaaattcag ttccccaccg cccagcttac ccatgcctaa aatcagcagc ggttgcgctt
     8581 cgccctgcgc attgcacggc gttccccatt cgcggcagca ggcgtcatac agccagtcac
     8641 gcgccgcaac aatcagcgtc tccgccagat ggctgagctg ctgcaatatg ctctcttcag
     8701 taaccagtgc cagcgtttgc gcccaggcga tgcgcaccat aatgcgccgc cggaatagcc
     8761 gcagctcgcg cattaacccg gcttcgtcac tcacattact gagcgcctcc tgcaaccatg
     8821 cagcgtaatg ctgccattcg tcggcctgcg gcggttggct ttccagttcc gtcagccact
     8881 ctggatgcgc aatcacgctg tcctgcacaa aatcactgaa agtaagtact gacttcgcct
     8941 gtgcgctaag tgattcctcg gctaaaggct ctggcagccg ctcaacaacg gtctgccagt
     9001 actgctgtaa cgatgaagag agcggcttca taagcgattt tatccttgcc tgattaacgt
     9061 tttccgctgt gcaaccagaa cggttcctga ttgtttgcct cattacggaa atgttcaatt
     9121 tcaatgcgtt gcccggtcgc aatagcgtga cgcagcccct gccagttctc cagccagtct
     9181 tgcgcgacga caggatcgta gtaacccgcc agcaacagta ttgagtcaat atcacgcgtc
     9241 aggcgtggca attggtcacg gtagcgatcg cctaacggct ggcagaaaac gcttttcagt
     9301 tcagcggcat ggcgggaaag atggatatcg gcaaagcgtt tgaaggaatc gctgattttg
     9361 ctctgcgctt tggcatctaa aaacggctgc caggctttgc ttaccagcca ttcggtcaac
     9421 gccagcttcg ccattgccgt ttcggtagag taggccgccg ttacggcaga aaccgcagaa
     9481 gcaatggtcg cctcgcattg agtcagcaga tcacgtaagt gagtgctcgc tttacgcggc
     9541 acgataccgc cgaacagcat cagtgtatga cggaccaggc caatggctgc cagcacctgc
     9601 tctttcgccg catcgttgcc gcgtacccac agttcttcat gatactgcca ttgcgctaac
     9661 gccagctcga acgccgcttc cagcccctgt tccacatcag cttttgccgc aacatgcaaa
     9721 atggtggtcg gtttgatttc acgcgccgga ttgccctgcg ccagatgata gccacgcgct
     9781 gctttgctca ggctgccctg gcgtaatccg gtttgcgata ccagttggtt cgccagtttc
     9841 agcaccgcac gcatgtcgcc gctaagcagt tccagttcca gctcacagat aggttcagca
     9901 aattcacccg ctttcacctc accctgatcg agagcgattt caattcggct gccatcgacc
     9961 tctactagcc atttttcgcg ataaaaatcg gtgctaaaca gcggctgtac gcgggaagcg
    10021 agatcggtag gcaattcgcc gttcggccag acttccgtcg gtaactgcgc caggtcgagc
    10081 gtcggttcgc tcaacgccac gttatattcc gggcgctggt gtaagccgcc tgtcactctt
    10141 cctgcaactt tcatggtcat ctcatagcga ccgttttcgc cacgaatacg caagcccata
    10201 tcgtgcccac gcagccagtt atccggcgtt tcgtagtaaa tattcagcaa ctgcacgggg
    10261 tcatggtgct cgccgtccag cgtattgaga tggtcacgca gcgcctcaac ggcactgtga
    10321 ttaacaataa actttaattc gatttcctga gccatagcct ttacttatcg gttatgtcac
    10381 ataagtgacg atgaacggcg aacttaatgc gatctttttt gtcagtagat agtattttgc
    10441 gccaaattgc catgcaacgg gcaatttgac gggcgtaaaa gtttgaagca gtggcaagac
    10501 ctgacacaga ggattccgat tgatgtcgaa tgctttgcgt agcgacactg atgccactac
    10561 tatcgttcca ctttccatga caataacgac agcctgatgc caaaattacg cctgatcgga
    10621 ttaactttac tcgcacttag cgccactgcc gtctcacacg ctgaagaaac gcgctatgtt
    10681 tctgatgaac tgaatacctg ggtccgtagc ggtccgggag atcattatcg cctcgtgggc
    10741 acggttaacg ccggcgagga agtgacctta ttacaaactg acgccaacac caattatgcc
    10801 caggtgaaag acagctctgg ccgtaccgcc tggatcccgt tgaaacaact tagcacggag
    10861 ccaagcctgc gttcccgtgt gccagatctg gaaaatcagg tcaaaacgct gaccgataaa
    10921 ctcaccaata tcgataacac ctggaatcag cgcaccgcag aaatgcagca aaaagtggcg
    10981 cagagtgaca gcgtgatcaa cgggttaaaa gaagaaaatc agaaactgaa aaacgagctg
    11041 attgtcgcgc agaaaaaggt cgatgccgcc agcgtacagc tggatgacaa acagcgcacc
    11101 atcatcatgc agtggtttat gtatggcggt ggcgtgctgg ggcttggctt gctgctcggt
    11161 ctggtactgc cgcacctgat cccaagccgc aaacgcaaag atcgctggat gaactaaatc
    11221 gccttcttcg ttgcactgtc ttattattag gaattgtgta tttgacgaag ggatgatggc
    11281 gtgaagattt atctggtcgg tggtgctgtt cgggatgcat tgttagggct accggtcaaa
    11341 gacagagatt gggtggtggt cggcagtacg tcacaggaga tgctcgacgc gggctaccag
    11401 caggtaggcc gcgattttcc tgtgtttctg catccgcaaa cgcatgaaga gtatgcactg
    11461 gcacgtaccg aacggaaatc cggttccggt tacaccggtt ttacctgcta tgccgcaccg
    11521 gatgtcacgc tggaagatga tcttaagcgt cgcgatctga ccattaatgc gctggcccag
    11581 gacgataacg gcgagattat cgacccgtac aacggtctga gcgatctgca aaatcgtctg
    11641 ttgcgccatg tttcccccgc ttttggcgaa gatccgttac gcgtgttgcg cgtagcgcgt
    11701 tttgctgcgc gttatgccca cctcagtttc cgtattgccg atgaaactct ggcgctgatg
    11761 cgcgagatga cccatgcggg tgaactggaa cacctgacgc ctgaacgggt atggaaagag
    11821 acggaaaacg ccctcaccac ccgcaatccc caggtgttct tccaggtact gcgcgattgc
    11881 ggcgcactgc gcgttttatt cccggaaatt gacgccctgt ttggcgttcc ggcccctgcc
    11941 aggtggcatc cggaaatcga cacgggtatt cataccttaa tgacgctctc aatggcggcg
    12001 atgctgagtc cgcaggtcga tgtccgtttc gcaacgttat gccacgatct cggtaaagga
    12061 ctgacgccgc cagaactttg gccacgtcat catggtcatg gcccggcggg tgtgaagtta
    12121 gtggaacaac tatgccagcg tctgcgcgtg ccaaatgaaa ttcgcgattt agccagactg
    12181 gtagctgagt ttcacgatct catccacacc tttccaatgc tgaacccgaa aaccattgtt
    12241 aaattgtttg attccattga cgcctggcgt aaaccgcagc gtgtcgagca actggcgctg
    12301 accagcgagg ctgacgtgcg cggcagaacc ggttttgaat cagcggacta cccgcaaggc
    12361 cgctggttgc gcgaagcctg ggaagtggcg cagtcagtgc cgacaaaagc tgtcgttgaa
    12421 gcaggattta aaggtgtgga gattcgtgag gagctgaccc gacggcggat tgcggcggta
    12481 gccagctgga aggaacagcg ttgcccaaaa cctgagtgag tgttggatat tttgccggat
    12541 gcggcgtaa
//
LOCUS       DADSYM010000139        11978 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2415
            SAMN08039579-rid11723173.denovo.140, whole genome shotgun sequence.
ACCESSION   DADSYM010000139 DADSYM010000000
VERSION     DADSYM010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039579
            Sequence Read Archive: SRR6362533
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11978)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11978)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980162.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 03:39:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,484
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 4,347
            CDSs (with protein)               :: 4,347
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 43 of 85
            Pseudo Genes (incomplete)         :: 37 of 85
            Pseudo Genes (internal stop)      :: 19 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11978
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039579-rid11723173.denovo.140"
                     /strain="LGE2415"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8302 N 95.9628 W"
                     /collection_date="2015-08-24"
     gene            211..1182
                     /locus_tag="J1Y83_003512"
     CDS             211..1182
                     /locus_tag="J1Y83_003512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000034588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DctP family TRAP transporter solute-binding
                     subunit"
                     /protein_id="HBA7631627.1"
                     /translation="MSKIIAMLITVSTLFLPSIIQAKPISIKVAYENNPGEPLDVVMR
                     YWADLLNKKSNGEITLALYPSSQLGSKQDVTEQAMMGMNVITLSDVAFLADYEPDLGI
                     LFGPYLTDDPQKLFKIYESDWFKQKNESLKKKGIHVVMNNYLYGTRQIISKKPIRTVD
                     DLAGLKIRVPNNVMQIKAIQAMGATPTPMPLGEVYPALTQGVIDGVENPISVLQGQKL
                     YEQARYLTMVNYLTNTSVWIGGEAFFGTLSPEQLEMIHQTGYEAGVYSQKLTLERDAE
                     MLKTMQAAGVEVIYPDTGPFQKKAREVYSQFPEWTPGLYETIQQQLQ"
     gene            1246..1719
                     /locus_tag="J1Y83_003513"
     CDS             1246..1719
                     /locus_tag="J1Y83_003513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001235677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HBA7631628.1"
                     /translation="MRLFGKVAEFSAAFALFVLVVVTIGAVFMRYFIGQPLQWTEEMS
                     GMLMIWVVMLGGVVAERDRAHLTIPFLMEMLPGKLRRVIAVLVALLSIALLLYMAWLG
                     YRLAEMAQFKVTQILKVSWFWIDLAVPVGALATAIYTLYWLINDTKQSDSGDKMQ"
     gene            1716..2987
                     /locus_tag="J1Y83_003514"
     CDS             1716..2987
                     /locus_tag="J1Y83_003514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000118634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HBA7631629.1"
                     /translation="MSWLLIIPLMIVCLFLNIRVYLAMFIGIIAYFLFFSSVPIEIAV
                     QRLIAPTQSPSLLAIPFFILLGTLMSYTGIAERILHIANVLVGKMRGGLGVANILVST
                     MMGGVSASNLADAAMLSRMMVPEMERKGYNRAFAAAITAGGSLVTPIIPPGIALIIYG
                     LVADVSIGKMFIAGLVPGLLCAVVLMFTVYLVARKTNAKPSRESWPTGKEVVFSLGQA
                     WPALFLIFAVVGGIRANIFTPTEAGAVAVLIVLAIGFFIYREMRISHVVKALGETARA
                     TASVMLVIMASAALGWIFSMEHAGVAVANFVTSLTENKYMFLLIINILLLTLGMFLEG
                     NAILLILVPLLKPVVEHFGIDPVHFGIIMIFNLSIGAFTPPVGTVMLLVCNITQVSVG
                     NFFKQSLPLLAALLLMLMLVTYIPAISLFLV"
     gene            complement(3030..4703)
                     /gene="tsr"
                     /locus_tag="J1Y83_003515"
     CDS             complement(3030..4703)
                     /gene="tsr"
                     /locus_tag="J1Y83_003515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HBA7631630.1"
                     /translation="MLKRIKIVTSLLLVLAVFGLLQLTSGGLFFNALKNDKENFTVLQ
                     TIRQQQFTLNGSWVALLQTRNTLNRAGIRYMMDQNNIGSGSTVAELMQSASISLKQAE
                     KNWADYEALPRDPRQSTAAAAEIKRNYDIYHNALAELIQLLGAGKINEFFDQPTQGYQ
                     DGFEKQYVAYMEQNDRLYDIAVSDNNASYSQAMWILVGVMIVVLAVIFAVWFGIKASL
                     VAPMNRLIDSIRHIAGGDLVKPIEVDGSNEMGQLAESLRHMQGELMRTVGDVRNGANA
                     IYSGASEIATGNNDLSSRTEQQAASLEETAASMEQLTATVKQNAENARQASHLALSAS
                     ETAQRGGKVVDNVVQTMRDISTSSQKIADIISVIDGIAFQTNILALNAAVEAARAGEQ
                     GRGFAVVAGEVRNLAQRSAQAAREIKSLIEDSVGKVDVGSTLVESAGETMAEIVSAVT
                     RVTDIMGEIASASDEQSRGIDQVGLAVAEMDRVTQQNAALVEESAAAAAALEEQASRL
                     TEAVAVFRIQQQQQQQQQQRETSAVVKTVTPATPRKMAMADSEENWETF"
     gene            5081..7231
                     /gene="btsT"
                     /locus_tag="J1Y83_003516"
     CDS             5081..7231
                     /gene="btsT"
                     /locus_tag="J1Y83_003516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate/proton symporter BtsT"
                     /protein_id="HBA7631631.1"
                     /translation="MDTKKIFKHIPWVILGIIGAFCLAVVALRRGEHVSALWIVVASV
                     SVYLVAYRYYSLYIAQKVMKLDPTRATPAVINNDGLNYVPTNRYVLFGHHFAAIAGAG
                     PLVGPVLAAQMGYLPGTLWLLAGVVLAGAVQDFMVLFISSRRNGASLGEMIKEEMGPV
                     PGTIALFGCFLIMIIILAVLALIVVKALAESPWGVFTVCSTVPIALFMGIYMRFIRPG
                     RVGEVSVIGIVLLVASIYFGGVIAHDPYWGPALTFKDTTITFALIGYAFVSALLPVWL
                     ILAPRDYLATFLKIGVIVGLALGIVVLNPELKMPAMTQYIDGTGPLWKGALFPFLFIT
                     IACGAVSGFHALISSGTTPKLLANETDARFIGYGAMLMESFVAIMALVAASIIEPGLY
                     FAMNTPPAGLGITMPNLHEMGGENAPIIMAQLKDVTAHAAATVSSWGFVISPEQILQT
                     AKDIGEPSVLNRAGGAPTLAVGIAHVFHKVLPMADMGFWYHFGILFEALFILTALDAG
                     TRSGRFMLQDLLGNFIPFLKKTDSLVAGIIGTAGCVGLWGYLLYQGVVDPLGGVKSLW
                     PLFGISNQMLAAVALVLGTVVLIKMKRTQYIWVTVVPAVWLLICTTWALGLKLFSTNP
                     QMEGFFYMASQYKEKIANGTDLTAQQIANMNHIVVNNYTNAGLSILFLIVVYSIIFYG
                     FKTWLKVRNSDKRTDKETPYVPIPEGGVKISSHH"
     gene            7349..7552
                     /locus_tag="J1Y83_003517"
     CDS             7349..7552
                     /locus_tag="J1Y83_003517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbdD/YjiX family protein"
                     /protein_id="HBA7631632.1"
                     /translation="MFGNLGQAKKYLGQAAKMLIGIPDYDNYVEHMKTNHPDKPYMSY
                     EEFFRERQNARYGGDGKGGMRCC"
     gene            7563..8519
                     /gene="yjiA"
                     /locus_tag="J1Y83_003518"
     CDS             7563..8519
                     /gene="yjiA"
                     /locus_tag="J1Y83_003518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase"
                     /protein_id="HBA7631633.1"
                     /translation="MNPIAVTLLTGFLGAGKTTLLRHILNEQHGYKIAVIENEFGEVS
                     VDDQLIGDRATQIKTLTNGCICCSRSNELEDALLDLLDNLDKGNIQFDRLVIECTGMA
                     DPGPIIQTFFSHEILCQRYLLDGVVALVDAVHADEQMNQFTIAQSQVGYADRILLTKT
                     DVAGEAEKLRERLARINARAPVYTVTHGDIDLGLLFNTNGFMLEENVVSTKPRFHFIA
                     DKQNDISSIVVELDYPVDISEVSRVMENLLLESADKLLRYKGMLWIDGEPNRLLFQGV
                     QRLYSADWDRPWGDEKPHSTMVFIGIQLPEDEIRAAFAGLRK"
     gene            complement(8553..8855)
                     /locus_tag="J1Y83_003519"
     CDS             complement(8553..8855)
                     /locus_tag="J1Y83_003519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006686551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnA antitoxin family protein"
                     /protein_id="HBA7631634.1"
                     /translation="MSMVKHKRGTPSTLDVQHEAELKALANKSDEDIDYSDIPPSSDE
                     QWSNAERGKFYRPLKTQASVRIDADVMEWLKRPGKGYQTRLNAILREAMLRDQNKK"
     gene            complement(8994..9728)
                     /locus_tag="J1Y83_003520"
     CDS             complement(8994..9728)
                     /locus_tag="J1Y83_003520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000208603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell filamentation protein Fic"
                     /protein_id="HBA7631635.1"
                     /translation="MTTKLLDLSWNANAIPAMQIVGTRAALFTFKTHMAGFVWDAAQL
                     ENNPYTFVEVKTLLDGVTVGGHKVSDTEQVLNLADSAKKLIELVQFGRFDLDKKTFTL
                     LNGIIARNEALEWGVFRGEGEELHYNARVHLGELGTHFPPATEAGAPELNRIFAAGVA
                     QIKEMPPFEGALAMFLFGAYFQFFFDGNKRTSRHMMNGWLMRHGYNPISVPAARALEF
                     NSKMVRFYHSKDASEMMAFLVDCYQV"
     gene            complement(9772..11871)
                     /locus_tag="J1Y83_003521"
     CDS             complement(9772..11871)
                     /locus_tag="J1Y83_003521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001531297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF262 domain-containing protein"
                     /protein_id="HBA7631636.1"
                     /translation="MKATEARLLDFLKRSQQFVIPIYQRTYSWSEEQCRQLWDDVIRA
                     GQRDDIPAHFIGSVVYIEQGLYQVSGISPLLVIDGQQRLTTAMLLLEALSRHLGDDEV
                     MDGFSAMKLRNYYLLNPYESGERGFKLLLTQTDRESLLALIRQKPLAENCSLRVKENF
                     EFFHEQIGKLHGNFTALCQGLSKLLIVDVALNRGQDNPQLIFESMNSTGKALSQADLV
                     RNYVLMGLEPEHQSQLYDDYWRPMELAFGQKGYTDYFDSFMRNYLTVKTGEIPRIGDV
                     YEAFKRYARKPGVMDSGMEALVADIRTYADYFCAMALDSEKDKILKMAFQDLRELKVD
                     AAWPFLLVLYHDYKQGILSAADFLSATRLIESYIFRGAVCAIPTNSFTKTFATFYKVL
                     DKERYLESIAAHLLELPSYRRFPDDDEFQRELKTRDLYNFRNRSYWLRRFENYDRKER
                     VYVDTYTIEHIMPQNPNLSAKWRDDLGPEWKRIHKKWLHTLGNLTLTGYNSSYSDRPF
                     AKKRDIKGGFKESPLRLNTGLGQCEIWDRAAIQKRAERLAELAIDVWAIPKLSEDVLA
                     RYRTVVERGTGYVLTDFPQLEEGTHICVLFDSLSDAVMTLDAGVTREILKLYVAFKAE
                     TNFVDVVPQKSRLRLSLNMPFHELVDPKCMAKDVTDIGRWGNGDVEVGFSDLSELPYV
                     MGLIRQAFEKQMDNAMA"
BASE COUNT         2880 a         3116 c         2893 g         3089 t
ORIGIN      
        1 atgcattaca taactttaag atatacaatc aaaaaatgca ttaaaaaccg attaaaagca
       61 gcaaagtatt acccggtgca cgaatttatg atacatctgc agccattctg ctattcgctg
      121 actacttttc agtaatgact gctttttcat aaggtttcac tcccgacaac aatgttggag
      181 ttttatttcc ttagcatgga gtaagtaaag atgagcaaaa taatagcgat gctaattact
      241 gtcagtaccc tttttttacc gtcaataatc caggcaaaac ctatttcaat aaaagttgcc
      301 tatgaaaata accctggaga acccctggat gtggtcatgc gttactgggc agacctatta
      361 aataaaaaaa gtaatggcga aataacactt gcgctctatc ccagctccca attgggatca
      421 aaacaggatg tcaccgagca agccatgatg ggcatgaatg tcataacctt gagtgatgtg
      481 gcttttcttg ccgattatga acccgattta gggattttgt ttggaccgta tctgactgac
      541 gatccgcaaa aactgttcaa aatatatgaa agcgactggt ttaaacaaaa aaatgaaagt
      601 ctgaagaaaa agggaattca tgtggtaatg aataattatc tctatggcac tcgacagatt
      661 atttcgaaaa aacccattcg caccgttgac gatctcgcag gtttaaaaat tcgcgttcct
      721 aataacgtca tgcaaataaa agccattcag gcgatggggg caacaccaac tcccatgcca
      781 ttaggcgaag tttatcccgc attaacccag ggcgttattg acggcgtaga aaacccaatt
      841 tcagtgctgc aagggcaaaa actgtatgag caggcaagat atctgacaat ggttaactac
      901 ctgacaaata cctcagtgtg gataggtggt gaagccttct ttggcaccct ttcaccagaa
      961 caactggaga tgatccacca gaccggctat gaagcgggtg tctatagcca gaaactcact
     1021 cttgagcgtg atgccgaaat gttgaaaacc atgcaggcag ccggcgtcga agtgatctat
     1081 cccgatactg gaccattcca gaagaaagca cgcgaagttt actctcaatt cccggaatgg
     1141 acaccaggtc tgtacgaaac cattcaacag caactgcaat aagcattaat gctcagcgtc
     1201 ctgcctcatc tctcttaggc aggacacgca ctaacaggag gtttcatgcg tctttttggc
     1261 aaagtggcag aatttagtgc tgcctttgcg ctattcgttc tggttgtggt gacaattgga
     1321 gcggtattta tgcgttattt catcggccaa ccgcttcaat ggacagaaga aatgtcggga
     1381 atgctgatga tttgggtggt gatgttaggt ggagtggtgg cggaacgtga cagggcgcac
     1441 ctgaccattc ccttcctgat ggagatgctt ccaggcaaac tcaggcgtgt tattgcagta
     1501 ctggtcgccc ttctctccat cgcactgctg ctttatatgg cctggctggg ctaccgacta
     1561 gccgaaatgg cgcagtttaa agtgacacaa attctgaagg tttcatggtt ctggatcgat
     1621 ctggcggtcc ctgtcggcgc actggcaacg gctatttata ccctctactg gttaataaac
     1681 gatacaaaac aatccgatag cggagacaaa atgcaatgag ttggttattg attattccgc
     1741 taatgatcgt ctgtttgttt ctcaacattc gggtctatct ggcgatgttt attggtatca
     1801 tcgcttactt tttattcttc tccagcgtac cgattgaaat tgccgtgcaa agactaatcg
     1861 cgccaacgca aagcccatca ttgctggcaa ttccgttttt tatcttgctc ggcacactaa
     1921 tgagctacac cggcattgcc gaacgcatcc tgcacattgc caacgttctg gtggggaaaa
     1981 tgcgcggcgg actgggcgtg gcaaacattc tcgtcagcac catgatgggc ggcgtcagcg
     2041 cctctaacct tgctgacgcg gcgatgctgt cgcgcatgat ggtgccggag atggaacgta
     2101 aaggctacaa tcgcgcgttt gccgccgcca ttaccgctgg tggctcgtta gtcaccccga
     2161 tcataccgcc tggaatagcc ctgattattt acggtctggt tgccgatgtc tctatcggaa
     2221 aaatgtttat cgccggactg gtacctggtt tgctatgcgc agtcgtactg atgtttaccg
     2281 tctaccttgt cgcccgcaaa actaacgcta aaccctcgcg tgaaagctgg cctacgggca
     2341 aagaggtggt gttctcgctg ggacaggcct ggcctgcgct gttcctcatt tttgctgtgg
     2401 tgggtggtat tcgcgctaac attttcaccc caacagaagc aggtgcagta gcggtgctaa
     2461 tcgtgctggc gattggcttt tttatctatc gtgaaatgcg gatttctcat gtcgtcaaag
     2521 cattgggcga aacggcacgc gcaaccgcat cggtgatgct ggtaatcatg gccagcgccg
     2581 cattgggatg gattttctca atggaacatg ctggtgtcgc agtggcgaat tttgtgactt
     2641 cgctgaccga aaacaaatac atgttcctgt tgattatcaa catattgctg ctgacgctgg
     2701 ggatgtttct ggaaggaaac gccattttgc tgatcctggt accactatta aaaccggtgg
     2761 tcgaacactt tggcatcgat ccggtgcatt ttggcatcat catgatcttc aacctgtcga
     2821 ttggcgcatt cacgccaccg gtaggtaccg tgatgctgct ggtatgtaat atcacgcaag
     2881 tttcggtggg aaatttcttt aaacaatccc tgcccctact ggcggctcta ctgttaatgc
     2941 taatgctggt gacatatatc ccggcgattt cactgttcct ggtttaataa taaaaacgcc
     3001 ggatgaaata ctcatccggc atcattacgt taaaacgttt cccagttctc ctcgctatct
     3061 gccatggcca ttttacgcgg cgtagctggt gtcacggttt ttaccacagc cgatgtttca
     3121 cgctgctgtt gctgctgttg ctgctgttgc tgaatccgga acactgccac tgcttcggtc
     3181 aggcgactgg cctgctcttc cagcgcggcg gcggcagcgg cagattcttc caccagcgcg
     3241 gcgttttgtt gggttacccg gtccatctca gcaaccgcta agccaacctg atcgatacca
     3301 cggctctgct catcagaagc agaagcaatt tcgcccataa tgtccgtcac gcgggtcacg
     3361 gcactgacaa tctccgccat tgtttccccg gcgctttcga ccagcgtaga gccaacatcc
     3421 actttcccca ccgagtcttc aatcaggctt ttaatttcac gagcagcctg agcgctacgc
     3481 tgggccagat tacgcacttc tcccgcgacc accgcaaaac cgcgtccttg ctcacccgca
     3541 cgcgcagcct caaccgccgc gttcaaagcc agaatattgg tctggaaggc aatgccgtca
     3601 attacgctga taatatcggc gattttctgc gaactggtgg agatatcgcg catggtctgc
     3661 accacgttat ccaccacttt gccgccgcgt tgcgccgttt cggaagcact taacgccaga
     3721 tggctggcct ggcgcgcatt ttcggcattc tgtttcaccg ttgcggtcag ttgctccatg
     3781 ctggctgccg tctcttccag cgaagcggcc tgttgctcgg tgcgcgaaga gagatcgtta
     3841 ttgccggtgg cgatttcgct ggcaccgcta tagatggcat tggccccgtt gcgcacatca
     3901 ccgacggtac gcatcagctc tccctgcata tggcgcaaac tctctgccag ttgccccatc
     3961 tcattagagc catccacctc aatcggtttc accagatcgc cgcctgcaat atgacgaatg
     4021 ctgtcaatca ggcgattcat cggcgcgacc agcgaggctt taataccgaa ccagacggcg
     4081 aagatgaccg ccagtacgac gatcatcacg cctaccagaa tccacatcgc ctggctgtag
     4141 gaggcattgt tatcgctgac ggcgatatca tagagccgat cgttttgctc catgtaagcc
     4201 acatactgct tctcgaaacc gtcctgatat ccctgggtcg gctgatcaaa gaactcgttg
     4261 attttgcctg cacctaacag ttgtatcagc tccgccagcg cattgtgata aatatcgtaa
     4321 ttacgtttga tctctgccgc tgcggcggtg ctctgacgcg ggtcacgcgg caacgcttcg
     4381 taatccgccc agtttttttc cgcctgtttc agcgaaatac tggcactctg catcagctca
     4441 gcaacggttg aaccgctacc aatattgttc tgatccatca tgtagcggat acccgcgcgg
     4501 ttgagggtgt tacgcgtctg caacaacgca acccagctgc cattcagcgt gaattgctgc
     4561 tggcgaatgg tttgtaaaac agtgaaattt tctttgtcat tctttaaggc attaaagaac
     4621 agaccgcctg atgtcagttg taaaaggcca aaaacggcca aaaccagcag taggctggtc
     4681 acaattttga tacgttttaa catggtttct ctttcctgtg gaccgatgca agattttcgg
     4741 cctggaaagg aaaaacttta tgaaaatccc gcggagatga cgccgagtaa ttttattcac
     4801 ccccctgaat ttaaaaagcc attatttaca taaattattc gcaatataaa tatgtgatat
     4861 aaatcacata tttatgatca cttaaacgac gcctttgccg ctcaccgcca ttcctgaccg
     4921 cttacgtccc taaaataacc actcagttat ttaccttact ttacgcgcgc gtaactctgg
     4981 caacatcact gcaggatagc ggtcaattta cctcctcaaa cgcaacgcaa acctagaacg
     5041 gcttcggcca actattaatc aatacatgcc aggttttact atggatacta aaaagatatt
     5101 caagcacata ccctgggtga ttctcggaat catcggtgca ttctgcctcg cggtagttgc
     5161 attacgtcgg ggggagcacg tcagcgccct gtggatcgtg gtcgcctctg tgtcggtgta
     5221 tctggtggct tatcgctact acagtctgta catcgcccag aaggtgatga aactcgaccc
     5281 cacgcgcgcg acgcctgcgg ttattaacaa cgacggtctg aactacgttc cgaccaaccg
     5341 ttacgtgttg tttggtcacc acttcgccgc tatcgccggt gctggtccgc tggtgggtcc
     5401 agttctcgcc gcgcagatgg gctacctgcc tggcacgctg tggctgctgg cgggggtagt
     5461 gctggccggt gcggttcagg actttatggt gctgtttatc tcctctcgcc gtaatggcgc
     5521 atctcttggt gagatgatca aagaagagat ggggccagta ccggggacta tcgcgctgtt
     5581 tggctgtttc ttaatcatga tcatcatcct cgccgttctg gcgctgattg tggttaaagc
     5641 cctggccgaa agtccgtggg gtgtcttcac cgtttgctca accgtaccga ttgcgctgtt
     5701 tatgggtatc tacatgcgct ttattcgtcc ggggcgtgtg ggtgaagtct ctgttattgg
     5761 tatcgtgctg ctggttgcct ctatctactt cggtggcgtg attgctcacg atccgtactg
     5821 gggtccggca ctgaccttta aagacaccac cattaccttc gcgctgattg gctatgcgtt
     5881 tgtttccgca ctgctgccag tgtggctgat cctcgcacca cgcgactatc tggcaacctt
     5941 cctgaaaatc ggcgttatcg tcggcctggc gctgggtatc gtggtgttga acccggaact
     6001 gaaaatgcct gcgatgaccc agtacattga cggtactggc ccgctgtgga aaggcgctct
     6061 gttcccgttc ctgttcatca ctatcgcctg tggtgcggta tctggcttcc acgcgctgat
     6121 ctcttccggt acgacgccaa aactgctggc taacgaaacc gacgcgcgtt tcatcggcta
     6181 cggcgcaatg ctgatggagt ccttcgtggc gattatggcg ctggttgctg cgtccatcat
     6241 cgaaccgggt ctttacttcg cgatgaacac cccgcctgct ggccttggca tcaccatgcc
     6301 taacctgcat gaaatgggtg gcgagaacgc gccgatcatc atggcacagc tgaaagacgt
     6361 taccgcacac gcggcagcga ccgtcagctc ctggggcttc gtgatttcgc cagagcagat
     6421 cctgcaaacc gcgaaagaca ttggtgagcc ttctgtcctg aaccgtgcag gtggtgcacc
     6481 aacgctggcg gtaggtatcg ctcacgtgtt ccacaaagtg ctgccgatgg ctgacatggg
     6541 cttctggtat cacttcggta ttctgttcga agccctgttc atcctgaccg cgctggatgc
     6601 gggtacccgt tctggccgct ttatgctgca agacctgctg ggtaacttca tcccgttcct
     6661 gaaaaaaacc gactccctgg ttgccggtat catcggtact gcgggctgtg tgggtctgtg
     6721 gggctacctg ctgtatcagg gcgtggtcga tccgctgggc ggcgttaaga gcctgtggcc
     6781 gctgttcggt atctccaacc agatgctggc agccgtggcg ctggtactgg gcaccgttgt
     6841 actgattaag atgaagcgca cccaatacat ctgggtaact gttgttccgg ctgtatggct
     6901 gcttatctgc accacctggg cgctgggcct gaaactgttc agcaccaacc cgcagatgga
     6961 aggcttcttc tacatggcaa gccagtacaa agagaagatt gctaacggta ctgacctgac
     7021 ggcgcagcag attgccaaca tgaaccacat cgttgtgaac aactacacca acgcaggcct
     7081 gagtattctg ttcctgattg tggtgtacag catcatcttc tacggtttca aaacctggct
     7141 gaaggtgcgt aacagcgaca aacgtactga caaagaaaca ccgtacgttc caatcccgga
     7201 aggcggcgtg aagatctctt cgcaccacta atcggttgcc ttatccggct tacaaaatgt
     7261 ctatcgtagg ccggataaag cgtttacgcc gcatccggca aatccccagc ccggaccttc
     7321 cgggctttac gcatatctgg aaggcaaaat gtttggtaac ttaggacagg caaaaaaata
     7381 tctcggccag gcggcgaaga tgttgattgg cattccagac tacgacaact acgtcgagca
     7441 tatgaagacc aaccatcccg acaagccgta catgagctat gaagaattct tccgcgagcg
     7501 ccagaacgcg cgctacggcg gcgatggtaa aggcggtatg cgctgttgtt aatggagaga
     7561 gcatgaaccc gattgcagtt accctactca ccggtttttt aggcgcgggc aaaaccaccc
     7621 tgctgcgcca tattcttaac gaacagcacg gctacaagat tgccgtaatt gaaaacgaat
     7681 ttggcgaagt ctccgttgac gatcaattaa ttggcgaccg cgccacgcaa atcaaaacgc
     7741 tgaccaacgg ctgcatctgc tgttcgcgct ccaacgagct ggaggacgcg ctactcgacc
     7801 tgctggataa tctcgacaag ggcaatattc agttcgaccg tctggtgatt gaatgcaccg
     7861 gcatggccga tcccggcccg attattcaga cctttttctc ccatgagatt ttatgccagc
     7921 gttacctgct ggacggcgtg gttgcgctgg tggatgcggt acatgccgat gagcagatga
     7981 accagttcac catcgcccag tcacaggttg gctacgccga ccgtattctg ctgacgaaaa
     8041 ccgacgtcgc aggcgaagca gaaaaactgc gtgaacgcct ggcgcgcatc aacgcccgtg
     8101 caccggtcta caccgtcacc cacggcgaca tcgacctggg tctgctgttc aataccaacg
     8161 gttttatgct ggaagagaac gtcgtcagca ccaaaccgcg tttccacttt atcgcggata
     8221 aacaaaacga tatttcgtcg attgtggtgg aactggatta cccggtagat atcagcgaag
     8281 tttcccgcgt gatggaaaac ctgctgctgg aatcggcaga taaactgctg cgttacaaag
     8341 ggatgctatg gattgacggc gaacctaacc gcctgctgtt ccagggcgtc cagcgcctct
     8401 acagcgccga ctgggaccgc ccgtggggcg atgaaaaacc gcatagtacg atggtgttta
     8461 tcgggattca gttgccggaa gatgagattc gggcagcgtt tgcggggttg aggaaataat
     8521 tttggatgat ggccgccatg tggcggcctg aattattttt tgttctgatc gcgcagcatc
     8581 gcttcacgta aaattgcgtt caggcgagtt tgatagcctt tacctggtcg cttcaaccat
     8641 tccatcacat cagcatcaat acgcaccgaa gcctgagttt tcaatggccg ataaaactta
     8701 ccgcgttctg cgttagacca ttgctcatcg ctggaaggtg ggatgtcgct gtaatcaata
     8761 tcctcgtcgg atttgttcgc cagcgccttc agttcggcct catgctgcac atcgagtgta
     8821 gagggtgtcc ctcgtttatg tttaaccatg ctcatagcgg ctcctctctt tacgatctgc
     8881 ttttcgtgca ctgatgattc gaatgttgga aatagcatca cgttttagga aaagataggg
     8941 aagttgatat gaaggggctg gatggcagca ttgggacacc atccagcact tattcaaacc
     9001 tgatagcaat ccaccaaaaa tgccatcatt tcactggcat ctttgctgtg ataaaagcgc
     9061 accattttgc tgttgaactc cagcgcacga gctgcgggta cactaatggg attgtaaccg
     9121 tggcgcatta accaaccgtt catcatatgg cgactggtac gcttgttgcc atcaaagaaa
     9181 aactgaaaat aagcaccaaa taaaaacatt gccagcgccc cttcgaacgg tggcatctct
     9241 tttatttgtg ccacacctgc ggcgaatatt cgattcagtt caggtgcacc tgcttcagtg
     9301 gctggtggaa aatgagtgcc cagttcaccg agatgaacgc gcgcattgta atgtaactct
     9361 tcaccttcgc cgcgaaaaac gccccactcc agcgcctcat tacgcgcaat aatgccgtta
     9421 agtaacgtga acgttttctt atcaaggtcg aaacgcccga attgcacaag ttcaatcagt
     9481 ttttttgcgc tatctgccag gttcaacacc tgctcagtat cactgacctt atgcccgcca
     9541 acagtgacac catcgagcag cgttttaact tcaacaaagg tataggggtt attttccagt
     9601 tgcgcggcat cccagacaaa ccctgccata tgcgttttga aggtaaacaa ggctgcgcgc
     9661 gtaccgacaa tttgcatcgc cgggatggca tttgcattcc agctcaggtc cagtagtttg
     9721 gttgtcatta gaggatcctt gattgcatgg taaagaggat attattccat attaagccat
     9781 cgcgttatcc atctgctttt caaatgcctg acgaattaat cccatcacat aaggaagctc
     9841 agaaagatcg ctgaaaccta cttcaacatc gccattgccc caacggccta tatctgtgac
     9901 atctttagcc atacattttg ggtcaaccag ttcatggaat ggcatattta gtgataaacg
     9961 taagcgactt ttttgcggca ccacatcaac aaaattcgtc tccgctttga aggcaacata
    10021 gagcttcaat atttctcgcg taaccccagc gtccagcgtc ataacggcat cagaaaggct
    10081 gtcaaaaagt acgcagatgt gcgtaccttc ttcaagttgc gggaaatcag taaggacata
    10141 tcccgttcct cgttcaacga cggtgcgata acgagccaac acgtcttccg aaagtttggg
    10201 gatcgcccag acgtctattg ccagctcggc cagacgttcc gctctctttt gtatcgctgc
    10261 acgatcccat atttcacatt gccctaaccc cgtgttcagg cgtaatggac tttccttaaa
    10321 gccacctttt atatctcgct tcttcgcaaa tggacggtcg ctgtagcttg agttatagcc
    10381 agttaacgtt aaattaccca acgtatggag ccacttttta tggatacgct tccattccgg
    10441 accaaggtca tcgcgccact tcgccgataa attagggttc tgcggcatga tatgttcaat
    10501 cgtataagtg tcaacataga cccgttcttt acgatcgtag ttttcaaatc tgcgtagcca
    10561 atagctgcga ttacggaagt tgtagagatc gcgagttttc agttcacgtt gaaattcatc
    10621 atcatccggg aaacgacggt aggaaggcag ttccagcaaa tgcgccgcaa tgctctccag
    10681 ataacgctct ttatccagca ctttgtaaaa cgtggcgaaa gttttagtaa atgagttggt
    10741 gggaatggca cagactgcgc cccggaagat atacgactca attaaacgcg tggcacttaa
    10801 aaaatccgcg gcagaaagaa ttccttgttt atagtcgtga taaagcacca acaggaacgg
    10861 ccaggcggca tcgactttca gctcacgtag atcctgaaac gccatcttta atattttgtc
    10921 tttttcgcta tccagcgcca tcgcacaaaa ataatccgca taggtacgaa tatccgcaac
    10981 cagcgcctcc atcccgctat ccatcactcc tggttttcgc gcatagcgtt taaaggcttc
    11041 atagacatca ccaattcgcg gaatctcacc tgtttttacc gtcaggtaat tacgcataaa
    11101 actgtcgaaa tagtcagtgt atcccttctg accaaatgcc agctccatgg gccgccagta
    11161 atcatcgtac agttggcttt gatgttctgg ctccagcccc atcagaacat agttgcgaac
    11221 cagatccgcc tgactcagcg cttttcctgt tgagttcatg ctttcaaaga ttagttgcgg
    11281 attatcctga ccgcgattca aggcgacatc cacaatcagc agtttggaaa ggccctgaca
    11341 taacgcggtg aaattgccgt ggagtttgcc tatttgctca tggaaaaact caaaattctc
    11401 tttcacgcgt agagagcaat tttctgccag cggtttttgt ctaatcaatg ccagcaggct
    11461 ctctctgtcc gtttgcgtaa gcagtaactt aaaaccacgc tcgcccgatt catacggatt
    11521 gagcagataa tagtttcgta atttcatggc ggagaaacca tccatcacct cgtcatcgcc
    11581 aaggtgtctg gacaacgctt ccagcaataa catcgccgtt gtcaggcgtt gctgaccgtc
    11641 aatcaccagt aagggagaga tccccgaaac ctgatatagg ccctgttcga tatacaccac
    11701 cgagccgata aaatgcgccg ggatatcatc acgctgcccg gcacgtatta catcgtccca
    11761 gagctggcgg cactgttcct ctgaccatga ataagtacgc tgataaatgg gaataacgaa
    11821 ttgctgagag cgttttagaa aatcgagtaa tcgggcttcg gtggctttca tgaatgactc
    11881 ctgtcaaatt gtgctggcaa acgacgtcat tatgctgcga ataaaaaata aaagttagcg
    11941 tctttgtttc tgcaaaaata ccattccagg ataaatca
//
LOCUS       DADSYM010000140        11886 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2415
            SAMN08039579-rid11723173.denovo.141, whole genome shotgun sequence.
ACCESSION   DADSYM010000140 DADSYM010000000
VERSION     DADSYM010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039579
            Sequence Read Archive: SRR6362533
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11886)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11886)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980162.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 03:39:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,484
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 4,347
            CDSs (with protein)               :: 4,347
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 43 of 85
            Pseudo Genes (incomplete)         :: 37 of 85
            Pseudo Genes (internal stop)      :: 19 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11886
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039579-rid11723173.denovo.141"
                     /strain="LGE2415"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8302 N 95.9628 W"
                     /collection_date="2015-08-24"
     gene            146..763
                     /locus_tag="J1Y83_003522"
     CDS             146..763
                     /locus_tag="J1Y83_003522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1287 domain-containing protein"
                     /protein_id="HBA7631637.1"
                     /translation="MKASLALFSLLTVFTSYSLKSPAVPPTVVQIQANTNLAIADGAR
                     QQIGSTLFYDPAYVQLTYPGGDVPQERGVCSDVVIRALRSQKVDLQKLVHEDMAKNFA
                     AYPQKWQLKRPDSNIDHRRVPNLETWFIRHDKTRPTSKNPSDYQAGDIVSWRLDNGLA
                     HIGVVSDGFARDGTPLVIHNIGAGAQEEDVLFSWRMVGHYRYFAK"
     gene            complement(790..1695)
                     /gene="yijE"
                     /locus_tag="J1Y83_003523"
     CDS             complement(790..1695)
                     /gene="yijE"
                     /locus_tag="J1Y83_003523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418378.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine transporter YijE"
                     /protein_id="HBA7631638.1"
                     /translation="MSAAGKSNPLAISGLVVLTLIWSYSWIFMKQVTSYIGAFDFTAL
                     RCIFGALVLFIVLLLRGRGMRPTPFKYTLAIALLQTCGMVGLAQWALVSGGAGKVAIL
                     SYTMPFWVVIFAALFLGERLRRGQYFAILIAAFGLFLVLQPWQLDFSSMKSAMLAILS
                     GVSWGASAIVAKRLYARHPRVDLLSLTSWQMLYAALVMSVVALLVPQREIDWQPTVFW
                     ALAYSAILATALAWSLWLFVLKNLPASIASLSTLAVPVCGVLFSWWLLGENPGAVEGS
                     GIVLIVLALALVSRKKKEAVSVKRI"
     gene            complement(1789..3969)
                     /gene="katG"
                     /locus_tag="J1Y83_003524"
     CDS             complement(1789..3969)
                     /gene="katG"
                     /locus_tag="J1Y83_003524"
                     /EC_number="1.11.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catalase/peroxidase HPI"
                     /protein_id="HBA7631639.1"
                     /translation="MSTSDDIHNTTATGKCPFHQGGHDQSAGGGTTTRDWWPNQLRVD
                     LLNQHSNRSNPLGEDFDYRKEFSKLDYYGLKKDLKALLTESQPWWPADWGSYAGLFIR
                     MAWHGAGTYRSIDGRGGAGRGQQRFAPLNSWPDNVSLDKARRLLWPIKQKYGQKISWA
                     DLFILAGNVALENSGFRTFGFGAGREDVWEPDLDVNWGDEKAWLTHRHPEALAKAPLG
                     ATEMGLIYVNPEGPDHSGEPLSAAAAIRATFGNMGMNDEETVALIAGGHTLGKTHGAG
                     PTSNVGPDPEAAPIEEQGLGWASTYGSGVGADAITSGLEVVWTQTPTQWSNYFFENLF
                     KYEWVQTRSPAGAIQFEAVDAPEIIPDPFDPSKKRKPTMLVTDLTLRFDPEFEKISRR
                     FLNDPQAFNEAFARAWFKLTHRDMGPKSRYIGPEVPKEDLIWQDPLPQPIYNPTEQDI
                     IDLKFAIADSGLSVSELVSVAWASASTFRGGDKRGGANGARLALMPQRDWDVNAAAVR
                     ALPVLEKIQKESGKASLADIIVLAGVVGVEKAASAAGLSIHVPFAPGRVDARQDQTDI
                     EMFELLEPIADGFRNYRARLDVSTTESLLIDKAQQLTLTAPEMTALVGGMRVLGANFD
                     GSKNGVFTDRVGVLSNDFFVNLLDMRYEWKATDESKELFEGRDRETGEVKYTASRADL
                     VFGSNSVLRAVAEVYASSDAHEKFVKDFVAAWVKVMNLDRFDLL"
     gene            complement(4298..5188)
                     /gene="metF"
                     /locus_tag="J1Y83_003525"
     CDS             complement(4298..5188)
                     /gene="metF"
                     /locus_tag="J1Y83_003525"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HBA7631640.1"
                     /translation="MSFFHASQRDALNQSLAEVQGQINVSFEFFPPRTSEMEQTLWNS
                     IDRLSSLKPKFVSVTYGANSGERDRTHSIIKGIKDRTGLEAAPHLTCIDATPDELRII
                     ARDYWNNGIRHIVALRGDLPPGSGKPEMYASDLVTLLKEVADFDISVAAYPEVHPEAK
                     SAQADLLNLRRKVDAGANRAITQFFFDVESYLRFRDRCVSAGIDVEIIPGILPVSNFK
                     QAKKFADMTNVRIPAWMAQMFDGLDDDAETRKLVGANIAMDMVKILSREGVKDFHFYT
                     LNRAEMSYAICHTLGVRPGL"
     gene            complement(5570..7123)
                     /locus_tag="J1Y83_003526"
     CDS             complement(5570..7123)
                     /locus_tag="J1Y83_003526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional metallophosphatase/5'-nucleotidase"
                     /protein_id="HBA7631641.1"
                     /translation="MKIKILAAGIALTLPFWACAKDVTIIYTNDLHAHVEPYKVPWIA
                     DGKRDIGGWANITTLVKQEKAKNKATWFFDAGDYFTGPYISSLTKGKAIIDIMNTMPF
                     DAVTIGNHEFDHGWDNTLLQLSQAKFPIVQGNVFYQNSSKSFWDKPYTIIEKDGVKIG
                     VIGLHGVFAFNDTVSAATRVGIEACDEIKWLQRYIDELKGKVDLTVALIHEGVPARQS
                     SMGGTDVRRALDKDIQTASQVKGLDILITGHAHVGTPEPIKVGNTLILSTDSGGIDVG
                     KLVLDYKEKPHNFTVKNFELKTIYADEWKPDQQTKQVIDGWNKKLDEVVQQTVAQSPV
                     ELKRAYGESASLGNLAADALLAAAGKNTQLALTNSGGIRNEIPAGAITMGGVISTFPF
                     PNELVTMELTGKQLRSLMEHGASLSNGVLQVSKGLEMKYDSSKPVGQRVITLTLNGKP
                     IEDATVYHIATQSFLADGGDGFTAFTEGKARNTTGGYYVYHAVVDYFKAGNTITDEQL
                     NGMRVKDIK"
     gene            7256..8380
                     /locus_tag="J1Y83_003527"
     CDS             7256..8380
                     /locus_tag="J1Y83_003527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001765453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytoplasmic protein"
                     /protein_id="HBA7631642.1"
                     /translation="MSTLFTINACKTFGCRNLGLASSEDYSWPDYKLGYPALHCRACG
                     SYPPLFDEQQFRDWLSVHLSTWASEKGHFCPVCYGTDMICYGHNPQGSQRIQCRNCKK
                     VWTPKQYQKEITHPQAIETVQLFIPFQGASAVQKLYVLVSLDATRGNILHLSTNYTQH
                     QTGDSLRYSYKGNTEPTMHHRDIVQKVDMREAQFLRRSQFDEIQYGSAVLKRNGKGAI
                     LRPVITAHGHFRILKIRYPDVKTHIISHECFLRGAIITAWADQFRQQQGELWFVEEEI
                     SDSNADTPWHFKGTTYHGWWQNQWQRWEQGNNCKMVCLLTGACLERGANVSLATSRCF
                     ITWLTDQHDFTQSALLSAGRVTQMLTSLALKYNESLTPSC"
     gene            8613..10163
                     /locus_tag="J1Y83_003528"
     CDS             8613..10163
                     /locus_tag="J1Y83_003528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001540354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional metallophosphatase/5'-nucleotidase"
                     /protein_id="HBA7631643.1"
                     /translation="MKTVFTTLSVIFGVIFSHSVLAQDVTIYYTNDIHAHVNPAKIIA
                     VDKNRLIGGMANIAGIVNEAKKKSKDVFFFDAGDYFTGPYISTLTKGEAIIDIMNTMP
                     FDAVSVGNHEFDHGVPNMVSQLSKAEFPILLGNIYYTDTNKPVWDHPWTIIEKDGLKI
                     GVIGLHGAFAFYDTVAAKAREGVEARDEIKYLNKALAELKGKVDITVLLIHEGVPARQ
                     SSFGSKDVERLLQADIETAKKVNGVDVLITGHAHVGTPQPIKVNNTLIVSTDAYGTNI
                     GKLVLDYNPKTKKIDSYNGELITIFADQFKPDTIVQNTIDKWSAKLNKITQEVVGHSP
                     VVLTREYGSSSSTGNLILDAMMEKTPDAVAGFQNSGGMRADFPKGDITLGDVISTFPF
                     NNDLIEMDLTGRDLKSLMTHATNLTNGVLQVSKSVAVVYDSKKPLNQRLISFTINGKP
                     VEDNQTYRIATHSFCASGGDGFEAFLNGKNVKTIPGTTSAESIIDYFKNHKPVTPDLT
                     KRVMDVAK"
     gene            10204..11823
                     /locus_tag="J1Y83_003529"
     CDS             10204..11823
                     /locus_tag="J1Y83_003529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001357039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional metallophosphatase/5'-nucleotidase"
                     /protein_id="HBA7631644.1"
                     /translation="MKTAQRTLLAATLLTIFSTSVMAQDVTIIYTNDLHAHVDSYKVP
                     YVADGKRDIGGFANISTLVKQEKAKNKATFYFDAGDYFTGPYISSLTKGEAIIDIMNT
                     MPFDAVSIGNHEFDHGWDNALRQLSKANFPVLLGNVYHKESEKPFWNKPYTILEKDGI
                     KIGIVGLHGVFAFNDTVSELSLQGLDNDNNNRFDKSSATLKNQGIEARDEVKYLQHYI
                     DELRDKVDLTVALVHEGVPARQSSIGNTDVRRALDKDIQTASKVKGLDILITGHAHVG
                     TPEPIKVGNTLILSTDSGGIDIGKLILDVNPTARTHKVKSFELKTVYADEWIPDPTTQ
                     KVINGWNKKLADLVRQPVGESSIALTRAYGESSQLGNLFTDAMLVAAPTAQIALINSG
                     SLRADINAGTITFGDITSTFPFKNELTEMDLSGKDLRNLLEHGASLTNGILQMSKGAE
                     MRYTLQKPVGQRIVSFKINGEEIVDTNIYHVATTTFLALGGDGFLAFKEGKNVQVRAG
                     NNMSNVVIDYFKKGHKITPAQVNEMRVEVSK"
BASE COUNT         3210 a         2948 c         2743 g         2985 t
ORIGIN      
        1 acgccgcatc cggcacgctg tgccagtaat ctgaaacttc caccttgttc gggtgttttt
       61 tatccgcttc tggacatttt ctccacagaa caatcgttag cccggaaggt cgaatctgtg
      121 actcttattt tcacggacga aacagatgaa agcttcactg gcgttattca gtcttctgac
      181 ggtatttacc agttactcct taaaatctcc cgccgttcca ccaaccgtgg tacaaatcca
      241 ggccaacacc aatcttgcca tcgcagacgg cgccagacag caaatcggca gcacgctatt
      301 ttacgatccg gcgtatgtgc agcttaccta tcctggcggt gatgtcccgc aagaacgcgg
      361 cgtatgttcc gatgtggtga tccgtgcatt gcgcagccag aaggtcgatt tgcagaaact
      421 ggtgcacgaa gatatggcga agaattttgc tgcgtacccg caaaaatggc agttaaagcg
      481 cccggacagc aacatcgatc accgtcgggt gcctaatctg gaaacctggt ttatccgcca
      541 cgataaaaca cgccccacca gcaagaaccc cagtgactat caagcgggcg atattgtctc
      601 ctggcgatta gataacgggc tggcacatat tggggtggtg tcagatggct tcgcccgcga
      661 cggtacgccg ctggtgatac ataacattgg cgcaggcgcg caggaagaag atgtactgtt
      721 tagctggcgg atggtggggc actatcggta ttttgctaaa taagagatct ccccacatga
      781 agaaaaaatt cagatccttt ttacactgac ggcttctttt ttcttacggc tcaccagcgc
      841 cagtgccagt acaatcagca caataccgct accttcaact gcccccggat tctcaccgag
      901 cagccaccag gaaaagagta cgccgcaaac gggaacggcc agtgtgctta agctggcaat
      961 actggctggc aagtttttca atacaaacaa ccataagctc cacgccagcg ctgtcgccag
     1021 aatcgcactg taggccagcg cccagaacac ggtgggttgc cagtcaattt cacgttgcgg
     1081 caccagtaaa gcgaccacgc tcatcaccag cgccgcatac agcatctgcc aggatgttag
     1141 cgacaataaa tccacgcgcg gatggcgggc atacagacgt ttagcaacaa tcgcgctcgc
     1201 cccccaactc acaccggaga ggattgccag catggcactt ttcatcgaag agaaatcgag
     1261 ttgccacggc tgcaacacca aaaataagcc gaaagcggca atcagtatcg cgaaatattg
     1321 cccacgtcgc aggcgttcac cgagaaacaa cgcggcgaaa atcaccaccc agaacggcat
     1381 ggtatagctc aggattgcca ccttccccgc tccgccgctg accagcgccc actgtgccag
     1441 gccaaccatc ccgcaggttt gtaacagggc aatggctaag gtgtatttaa acggcgtcgg
     1501 gcgcattccg cgaccacgta ataaaaggac gatgaataaa acgagagcgc cgaaaatgca
     1561 gcgtaaggcg gtgaagtcga aggcaccgat gtagctggtg acttgcttca tgaaaatcca
     1621 gctataactc cagataagtg tgagcacaac caggccactg attgccagtg ggttgctctt
     1681 tcctgcggca gacatgagag atccagtgtg tagttttcct gtggacacta tacgccggta
     1741 aagaaacttc agcgactgca tgaaaggcag tcgctgaaga aggtcagatt acagtaggtc
     1801 gaaacggtcg aggttcatca ctttcaccca cgctgccacg aagtctttaa caaacttctc
     1861 gtgggcatcg ctgctggcgt aaacttccgc caccgcacgc aagacagagt tagaaccaaa
     1921 gaccagatcc gcacggctgg ctgtgtattt cacttcgcca gtttcgcggt cacggccttc
     1981 gaacagttct ttcgattcgt cggtcgcttt ccactcgtaa cgcatatcca gcaagttcac
     2041 gaagaagtca ttgctcaata caccaacgcg gtcagtgaag acaccgtttt tgctgccatc
     2101 gaagttggca cccagtacgc gcatcccgcc caccagcgca gtcatttccg gcgcggtcag
     2161 cgtcagttgc tgtgctttat caatcaacag tgactcggtg gtggaaacgt ccagacgagc
     2221 gcggtagtta cggaaaccgt cagcaattgg ctccagcagc tcaaacattt caatgtcagt
     2281 ctgatcctga cgcgcatcaa cgcggcccgg cgcaaacggt acatgaatgc tcaaacctgc
     2341 ggcgcttgcg gctttctcaa cgccaaccac acctgccagc acgatgatat ctgccagcga
     2401 ggctttacca gactctttct gaattttctc cagaactggc agagcacgaa cggctgcggc
     2461 gttcacatcc cagtcacgct gcggcattaa tgccagacgc gcaccgttgg caccaccacg
     2521 tttgtcgcca ccacggaagg tagaagcaga cgcccaggcc accgatacca gctcactaac
     2581 agacagacca gaatccgcaa tcgcgaattt cagatcgata atgtcctgct cggtcgggtt
     2641 gtagatcggc tgcggcagcg gatcttgcca gatcagatct tctttcggta cttccggccc
     2701 gatgtaacga gatttcggcc ccatatccct gtgcgtcagt ttgaaccagg cacgagcaaa
     2761 ggcttcgttg aacgcctgcg gatcgttgag gaaacgacga gagatcttct cgaactcagg
     2821 atcaaaacgc agcgtcaggt cggtcaccag catcgtcggt ttacgtttct tcgacggatc
     2881 aaacggatcc gggataattt ccggtgcgtc taccgcttca aactggattg caccagccgg
     2941 gctgcgggtc tgtacccact catacttgaa caggttctcg aagaaatagt tgctccactg
     3001 ggtcggcgtc tgggtccaga ccacttccag accagaggta atggcatctg cgccaacgcc
     3061 gctgccgtaa gtgctcgccc agcctaaacc ttgttcttca atcggtgcag cttctggatc
     3121 aggacctacg tttgatgtcg gaccagcacc gtgggtttta cccagcgtat gaccacccgc
     3181 aatcagcgcc acggtttctt cgtcattcat gcccatgttg ccgaaggtcg cgcggatagc
     3241 tgctgctgcc gaaagtggtt cgccgctgtg atccgggcct tccgggttaa cgtaaatcag
     3301 gcccatctcg gttgcaccca gcggtgcttt cgccagcgct tccggatgac ggtgagtcag
     3361 ccaggctttt tcatcacccc agttaacatc cagatccggt tcccagacgt cttcacgacc
     3421 ggcaccaaaa ccgaaggtac ggaagccgga gttttccagc gccacgttac ccgcgaggat
     3481 aaacaggtcg gcccaggata ttttctgacc atatttctgt ttgattggcc acaacaggcg
     3541 acgcgcttta tcgaggctta cgttatccgg ccaggagttc agcggtgcaa aacgttgctg
     3601 accacgaccc gcgccgccgc gaccgtcgat tgaacggtaa gtacccgcgc cgtgccaggc
     3661 catacgaata aacagaccgg cgtagctgcc ccagtcggct ggccaccacg gttgagattc
     3721 tgtcagcagg gctttcagat cttttttcag gccgtagtaa tctaatttgc tgaattcttt
     3781 gcggtagtca aagtcctcac ccagcgggtt agaacgatta gaatgttggt ttaacaggtc
     3841 aacacggagt tgatttggcc accagtcgcg cgtggttgtg cccccacctg cgctctggtc
     3901 gtgaccgccc tgatggaacg ggcatttgcc ggtggctgtc gtgttatgga tatcgtctga
     3961 cgtgctcatc aatgtgctcc cctctacagt gttaccgtta cgatacacag cgttagagag
     4021 aagttataat tgaattaatc cacggatgcg atagttgaga tcttacatat tgttggttaa
     4081 agagatgtag atcaaattga tcgtaattaa gactttttga ttatgtccga tttaggataa
     4141 atgctttata aaaaaaggtt ttagatttgt atccttcaag taatgaggga gaaaacaagg
     4201 ctgataacct tattttcccg ccctcatttc gaggcagcat tttgtgctct gtttaaaatt
     4261 tgtgatcact gtgtgatttt cacaaaaggc acactattta taaaccaggt cgaacaccca
     4321 gtgtatggca aatcgcgtaa ctcatttcag cgcggttaag cgtatagaag tggaaatctt
     4381 tcactccttc acggcttaaa atcttcacca tatccatggc aatattcgcg cccaccagct
     4441 tgcgggtttc agcatcgtca tccaggccgt cgaacatttg tgccatccac gccggaatac
     4501 gcacgttggt catgtcggca aatttcttcg cctgtttaaa gttagatacc ggcaaaatgc
     4561 ccggaatgat ttccacatca atgcctgccg agacgcagcg atcgcgaaaa cgcaggtagc
     4621 tttcgacatc gaagaagaac tgagtaatgg cgcggttggc tccggcatcc acttttcgtc
     4681 tcaggttgag taaatcggcc tgggcacttt tcgcttctgg atgtacttcc ggatacgccg
     4741 ccacggagat atcgaaatct gccacttctt ttaacagcgt caccaggtca gaagcataca
     4801 tttctggctt accacttccc ggcggcaggt cgccacgcag cgccacgata tggcgaatac
     4861 cgttattcca gtagtcgcgt gcaatgatcc gcagctcgtc gggcgtcgca tcaatacagg
     4921 taagatgcgg tgccgcttcc aggccggtgc gatctttaat gcctttaata atgctgtgcg
     4981 tacggtcgcg ctcgccggag tttgcgccat aggtcaccga tacaaacttc ggtttcaggc
     5041 tgctaaggcg atcgatggag ttccacaggg tctgctccat ttcactggta cgcggcggga
     5101 aaaattcgaa cgaaacgtta atctgcccct ggacttctgc cagactctga ttcagggcat
     5161 cccgctggct ggcgtgaaaa aagctcatac cttacctcat caatcgctta tcgttatatg
     5221 ttgtgttgtg cacatctatc cgtttagacg tccagatgta aagatgaagg aaaagccgaa
     5281 gggcgtcaac cgaaaaataa ccaatgatgg tgaggaaaaa tcagggaaga tgaaaaaaac
     5341 tcatcttaat tagaggcgat aaaagatttc tctatagcgc aatccacctc gcacgttttg
     5401 actcatattc tgacaacaca gcgacacctg ggctaattct aaaaacaacg tcctgccacc
     5461 ggatggtgat gctaaggcat ctgatttggc ctgcgccctc gcgtaggccg aatacaggat
     5521 ctgcgcaatc aacgggtaac aaacagttga gtcaattcag tgcgcacttt tatttgatat
     5581 ccttcacgcg cataccgttg agctgttcat ccgtaatggt gttgcccgct ttgaagtaat
     5641 caaccaccgc gtgataaacg taataaccgc ccgttgtgtt acgcgctttc ccttcggtaa
     5701 aggcggtaaa accatctcca ccatcagcaa ggaaactctg agtagcaatg tgataaaccg
     5761 tcgcatcttc aatgggtttg ccattcagag tcagtgtgat tacccgctga ccaaccggct
     5821 tgctgctgtc gtacttcatt tccaggcctt tcgatacctg taaaacgcca ttgcttaaac
     5881 ttgcgccgtg ttccatcaga ctgcgtaatt gtttacccgt gagttccatc gtcaccagtt
     5941 cgttggggaa cgggaacgta ctgatgacgc cgcccatcgt gatcgcccct gcggggatct
     6001 cattgcggat accaccagag tttgttaaag ccagttgggt atttttaccc gctgctgcca
     6061 gtaaagcgtc agccgccagg ttcccgaggg aagccgattc accataggca cgttttaatt
     6121 caaccggcga ttgcgccacc gtttgttgca ccacttcatc aagcttttta ttccagccgt
     6181 cgatcacctg tttcgtttgc tgatcgggct tccactcgtc agcgtaaatg gttttaagct
     6241 cgaagttttt caccgtaaaa ttatgcggct tctctttgta gtcgagaacc agtttgccca
     6301 catcaatccc gccgctgtca gtggagagga tgagcgtatt gccgacttta atcggttccg
     6361 gcgtacccac atgtgcatgt ccggtgatca aaatatccag ccctttcacc tgacttgccg
     6421 tctgaatatc tttatccaac gcgcgacgca catctgtgcc tcccatactg gactggcggg
     6481 ctggaacacc ttcgtggatc agggcgacgg tcaaatcaac cttgccttta agttcatcga
     6541 tataacgttg tagccattta atttcatcac acgcctcaat ccccactcgc gtggcagcag
     6601 atactgtgtc attaaaggca aatacaccgt gcaaacctat aacgcctatt ttcacaccgt
     6661 ctttttcgat gatggtatag ggcttatccc agaatgattt actgctgttc tgataaaaaa
     6721 cattgccctg cacaatcggg aattttgcct gactcaactg taataatgtg ttgtcccagc
     6781 cgtggtcaaa ttcatggttg cctatcgtga cggcatcaaa cggcatagta ttcataatat
     6841 cgataatagc cttacctttg gtcaggctgc tgatatatgg cccggtaaaa tagtcgcccg
     6901 catcaaaaaa ccatgtcgct ttattttttg ctttttcttg cttaaccagc gtagtgatat
     6961 ttgcccagcc gccaatatcc cgtttaccgt cagcgatcca cggcacttta taaggttcta
     7021 catgcgcatg gaggtcgttg gtataaataa tagtgacatc tttagcgcaa gcccagaacg
     7081 gcagtgttaa tgcgataccc gcagcaagta ttttaatttt cataaacaat ccctttcagt
     7141 gagataaagg attgctacat tacgttctga aagacttatt tatgtgagag taatcacatc
     7201 cgcaggggat agttgccgga caaaatcaac gaccttgtga aatcgttggt gatatatgtc
     7261 gactctgttt acgattaatg cctgtaaaac gttcggttgc cgaaatttgg gattggcctc
     7321 atcggaagat tacagttggc cagactataa actgggttat ccggcactgc actgtcgggc
     7381 atgtggcagt tacccgccgt tgtttgacga gcaacaattt cgtgactggt tgtcagttca
     7441 cttatctacg tgggcctcgg agaagggtca tttctgcccg gtatgttatg gcacagacat
     7501 gatttgttat ggtcacaatc cacagggttc gcaacgcatt caatgccgta actgcaaaaa
     7561 agtctggacg cctaaacagt atcagaaaga aattacgcat ccccaggcca ttgaaaccgt
     7621 tcaactgttc ataccctttc aaggggccag cgcagtacaa aaactgtatg ttttagtcag
     7681 tcttgacgcc actcgtggca acattcttca tctctccacg aattatacgc aacatcagac
     7741 aggagacagt ctgcggtaca gttacaaggg caacacagaa ccaactatgc accatcgcga
     7801 tatcgtgcag aaggtagata tgcgtgaagc gcaattcttg cgccggagcc agttcgatga
     7861 aattcagtat ggcagtgctg tactcaagcg caacggtaag ggagccatac tacgcccggt
     7921 tatcacggca cacgggcatt tcagaatact gaaaatccgc tatccagatg tcaaaacgca
     7981 tattatttca cacgaatgtt ttctaagagg cgcaattatt acagcctggg cagatcagtt
     8041 ccgccaacaa caaggtgaac tttggttcgt agaagaagaa atcagtgaca gtaacgctga
     8101 tacgccctgg cattttaagg gaacgacata ccatggttgg tggcaaaatc agtggcagcg
     8161 ctgggagcag gggaataact gcaagatggt ctgcctactc acgggagcct gcttagaaag
     8221 gggcgcaaat gtttctctgg caaccagtcg ttgttttatc acatggctga cagaccaaca
     8281 cgactttacc caaagcgcgt tattatccgc aggtcgcgta acgcaaatgc tgacctcact
     8341 ggcgttaaaa tacaatgaat cgctcactcc atcttgttag tcaatacact cgacaaacga
     8401 ccataaatta agagagatca ctacctttca tttaattaaa tatataaatg tatgaccgtg
     8461 aaatataatc atggtcaatg tttaattaat aacattattt gttactaaga tcacacaata
     8521 cttttctgaa ctatgcttac ataaaataag acacgatgtg tcttttattt aaataattca
     8581 acaaatgtaa caacccctat ccaggaggga tgatgaaaac tgtatttaca acattatcag
     8641 tcatattcgg cgtgatattt tctcacagtg ttctagctca ggacgttact atttattata
     8701 cgaatgacat tcacgcacat gtcaatccag caaaaatcat cgctgttgat aagaacagac
     8761 ttataggtgg tatggctaat attgcaggca tcgttaatga agcgaagaaa aaaagcaaag
     8821 atgtgttttt ctttgatgca ggcgactatt ttaccgggcc gtatatcagc accctgacca
     8881 aaggtgaagc tattattgat atcatgaaca ctatgccctt tgatgcggtg tctgttggta
     8941 atcacgagtt cgatcacggt gtgcccaata tggtgtctca gttatcaaaa gcagaattcc
     9001 ccattctgct gggaaatatc tactacaccg atacgaataa accagtttgg gatcatccgt
     9061 ggaccattat cgaaaaagat ggtttaaaga ttggtgtgat tggattgcat ggcgcattcg
     9121 ctttctatga tacagtcgcg gcaaaagcac gtgaaggcgt agaagccaga gacgaaatta
     9181 aatatttaaa taaagcactt gcagaattaa aaggtaaagt cgacataacc gttttgctaa
     9241 ttcacgaagg tgtcccggcg cgtcaatcaa gctttggtag taaagatgtg gagcggctac
     9301 tacaggcaga tattgagaca gctaaaaaag tgaatggtgt tgatgtctta attaccggtc
     9361 acgcacatgt cggaacaccg caaccaataa aagttaataa cacattaatt gtatctaccg
     9421 atgcatacgg caccaacatc ggaaaacttg tgcttgatta caatccgaaa acgaagaaaa
     9481 ttgatagtta taatggtgag ttaatcacca tctttgcaga tcaatttaaa cctgacacca
     9541 tcgttcaaaa tactattgat aaatggagtg caaagcttaa caaaataaca caggaagttg
     9601 tcggccattc tcccgtagtt ttaacacgtg agtatggtag ttcctcttct accggcaacc
     9661 ttattcttga tgcaatgatg gaaaaaacac ctgatgccgt tgccggattt caaaatagcg
     9721 gtgggatgcg agctgatttt cctaaaggcg atatcacact gggagatgtt attagcacat
     9781 tcccctttaa taatgacctc atcgagatgg atttgacggg ccgcgatctc aaatcattga
     9841 tgacgcatgc aaccaatcta actaacggtg tgttacaagt ttctaaaagt gttgcggttg
     9901 tctatgacag caaaaaacca ctcaaccaac ggttaatctc tttcaccatt aacggcaaac
     9961 ccgtggaaga taatcaaaca tatcgtattg ccacgcactc cttttgtgcc agtggtggtg
    10021 acggttttga agcatttttg aatggaaaaa atgtgaagac gataccggga acaacctcgg
    10081 cggaatctat catcgattat ttcaaaaatc ataaacctgt caccccagac ttaactaaac
    10141 gagtcatgga cgtcgccaaa taaaatctca tttatcagtc aatttattta acaaggaatg
    10201 cgaatgaaaa cagcgcagcg tactttactt gccgcaacat tgctgactat tttttcgacc
    10261 tcagttatgg cgcaggatgt cacgattatt tataccaatg atcttcacgc tcatgtggat
    10321 tcctataaag taccgtatgt cgcagacgga aaacgcgaca ttggtggctt tgccaatata
    10381 tccacgttag taaaacaaga gaaagccaaa aataaagcca ccttttattt tgatgctgga
    10441 gactatttca ccgggccata tatcagtagc ctgacaaagg gcgaagcaat aattgatatt
    10501 atgaatacca tgccttttga cgcagtatca atcggtaatc atgagtttga ccatggctgg
    10561 gataatgccc tccgacaatt aagcaaagcg aatttccctg ttttattagg caatgtttat
    10621 cataaagaga gtgagaaacc cttctggaac aagccctaca ccatcctgga aaaagatggc
    10681 atcaagattg gtattgttgg gctacacggc gtatttgcat ttaatgacac ggtctcggag
    10741 ctctctctcc agggactcga taatgacaat aataaccgat ttgataaatc ttccgcgacc
    10801 ctcaagaatc aggggattga agcacgtgat gaagtgaagt atttgcagca ttatatcgac
    10861 gagttacgtg ataaggttga cctcaccgtg gcgctggttc atgaaggcgt accggcgcgt
    10921 cagtccagca ttggcaatac agatgttaga cgtgcgctgg ataaagatat tcaaaccgca
    10981 agcaaggtga aaggattgga tattctgatt accgggcatg cccacgtcgg aacgccagaa
    11041 ccaatcaaag tcggcaacac cttaatcctt tcgactgaca gtggcggtat cgatattggt
    11101 aaattaatcc ttgatgtcaa ccctacagct cggactcata aagtgaagag ctttgagctg
    11161 aaaacggttt atgccgatga atggataccc gatccaacca cacaaaaagt tatcaatggc
    11221 tggaataaaa aactggcgga tcttgtacgc caaccagtcg gtgaatcatc gattgcatta
    11281 actcgtgcct acggtgagtc atcgcaactt ggcaacctgt ttaccgatgc aatgcttgtc
    11341 gctgcaccga ctgcacaaat tgcgttgata aactccggca gtttacgtgc tgatataaat
    11401 gcaggcacga ttacctttgg tgacattacc agtacattcc ccttcaagaa tgaacttact
    11461 gaaatggatc tcagcggtaa ggatctgcgt aacctactgg agcacggggc atcgcttacc
    11521 aatggcatcc tgcaaatgtc aaaaggcgca gaaatgcgct ataccctgca gaaaccagta
    11581 ggtcagcgca ttgtatcttt taaaattaat ggtgaagaga tcgttgatac gaatatttac
    11641 catgttgcga ctacgacttt ccttgcactt gggggcgatg gttttctggc attcaaagaa
    11701 gggaaaaatg tccaggtccg cgccggaaat aacatgtcca atgtcgtaat cgattatttt
    11761 aagaaaggcc acaagattac tcctgcacag gtaaatgaaa tgcgggtaga agtaagcaaa
    11821 taacaaaaac ttataatacc attcattcac tggatgacat tatttctctc taaatcaaca
    11881 atatat
//
LOCUS       DADSYM010000141        11835 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2415
            SAMN08039579-rid11723173.denovo.142, whole genome shotgun sequence.
ACCESSION   DADSYM010000141 DADSYM010000000
VERSION     DADSYM010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039579
            Sequence Read Archive: SRR6362533
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11835)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11835)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980162.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 03:39:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,484
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 4,347
            CDSs (with protein)               :: 4,347
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 43 of 85
            Pseudo Genes (incomplete)         :: 37 of 85
            Pseudo Genes (internal stop)      :: 19 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11835
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039579-rid11723173.denovo.142"
                     /strain="LGE2415"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8302 N 95.9628 W"
                     /collection_date="2015-08-24"
     gene            <1..587
                     /locus_tag="J1Y83_003530"
     CDS             <1..587
                     /locus_tag="J1Y83_003530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YebB family permuted papain-like enzyme"
                     /protein_id="HBA7631645.1"
                     /translation="AEYEIGDIVFTCIGAALFGQISAASNCWSNHVGIIIGHNGEDFL
                     VAESRVPLSTITTLSRFIKRSANQRYAIKRLDAGLTEQQKQRIVEQVPSRLRKLYHTG
                     FKYESSRQFCSKFVFDIYKEALCIPVGEIETFGELLNSNPNAKLTFWKFWFLGSIPWE
                     RKTVTPASLWHHPGLVLIHAVGVETPQPELTEAV"
     gene            complement(589..1110)
                     /gene="ruvC"
                     /locus_tag="J1Y83_003531"
     CDS             complement(589..1110)
                     /gene="ruvC"
                     /locus_tag="J1Y83_003531"
                     /EC_number="3.1.22.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005068993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HBA7631646.1"
                     /translation="MAIILGIDPGSRVTGYGVIRQVGRQLSYLGSGCIRTKVDDLPSR
                     LKLIYAGVTEIITQFQPDYFAIEQVFMAKNADSALKLGQARGVAIVAAVNQELPVFEY
                     AARQVKQTVVGIGSAEKSQVQHMVRTLLKLPANPQADAADALAIAITHCHVSQNAMQM
                     SESRLNLARGRLR"
     gene            complement(1145..1885)
                     /locus_tag="J1Y83_003532"
     CDS             complement(1145..1885)
                     /locus_tag="J1Y83_003532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000907250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBA7631647.1"
                     /translation="MAGHSKWANTRHRKAAQDAKRGKIFTKIIRELVTAAKLGGGDPD
                     ANPRLRAAIDKALSNNMTRDTLNRAIARGVGGDDDANMETIIYEGYGPGGTAIMIECL
                     SDNRNRTVAEVRHAFSKCGGNLGTDGSVAYLFSKKGVISFEKGDEDTIMEAALEAGAE
                     DVVTYDDGAIDVYTAWEEMGKVRDALEAAGLKADSAEVSMIPSTKADMDAETAPKLMR
                     LIDMLEDCDDVQEVYHNGEISDEVAATL"
     gene            complement(1914..2366)
                     /gene="nudB"
                     /locus_tag="J1Y83_003533"
     CDS             complement(1914..2366)
                     /gene="nudB"
                     /locus_tag="J1Y83_003533"
                     /EC_number="3.6.1.67"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate diphosphatase"
                     /protein_id="HBA7631648.1"
                     /translation="MTDMAYKRPVSILVVIYAQDTKRVLMLQRRDDPDFWQSVTGSVE
                     EGETAPQAAMREVKEEVTIDVVAEQLTLIDCQRTVEFEIFSHLRHRYAPGVTRNTESW
                     FCLALPHERQIVFTEHLAYKWLDASAAAALTKSWSNRQAIEQFVINAA"
     gene            complement(2359..4131)
                     /gene="aspS"
                     /locus_tag="J1Y83_003534"
     CDS             complement(2359..4131)
                     /gene="aspS"
                     /locus_tag="J1Y83_003534"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HBA7631649.1"
                     /translation="MRTEYCGQLRLSHVGQQVTLCGWVNRRRDLGSLIFIDMRDREGI
                     VQVFFDPDRADALKLASELRNEFCIQVTGTVRARDEKNINRDMATGEIEVLASSLTII
                     NRADVLPLDSNHVNTEEARLKYRYLDLRRPEMAQRLKTRAKITSLVRRFMDDHGFLDI
                     ETPMLTKATPEGARDYLVPSRVHKGKFYALPQSPQLFKQLLMMSGFDRYYQIVKCFRD
                     EDLRADRQPEFTQIDVETSFMTAPQVREVMEALVRHLWLEVKGVDLGDFPVMTFAEAE
                     RRYGSDKPDLRNPMELTDVADLLKSVEFAVFAGPANDPKGRVAALRVPGGASLTRKQI
                     DEYGNFVKIYGAKGLAYIKVNERAKGLEGINSPVAKFLNAEIIEAILERTGAQDGDMI
                     FFGADNKKIVADAMGALRLKVGKDLGLTDESKWAPLWVIDFPMFEDDGEGGLTAMHHP
                     FTSPKDMTAAELKAAPENAVANAYDMVINGYEVGGGSVRIHNGDMQQTVFGILGINEE
                     EQREKFGFLLDALKYGTPPHAGLAFGLDRLTMLLTGTDNIRDVIAFPKTTAAACLMTE
                     APSFANPTALAELSIQVVKKAENN"
     gene            4441..5007
                     /locus_tag="J1Y83_003535"
     CDS             4441..5007
                     /locus_tag="J1Y83_003535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HBA7631650.1"
                     /translation="MLELNAKTTALVVIDLQEGILPFAGGPHTADEVVNRAGKLAAKF
                     RANGQPVFLVRVGWSADYAEALKQPVDAPSPAKVLPENWWQHPAALGATDSDIEIIKR
                     QWGAFYGTDLELQLRRRGIDTIVLCGISTNIGVESTARNAWELGFNLVIAEDACSAAS
                     AEQHNNSINHIYPRIARVRSVEEILHAL"
     gene            5004..5822
                     /locus_tag="J1Y83_003536"
     CDS             5004..5822
                     /locus_tag="J1Y83_003536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HBA7631651.1"
                     /translation="MIYIGLPQWSHPKWVRLGITSLEEYARHFNCVEGNTTLYALPKP
                     EVVLRWREQTTDDFRFCFKFPATISHQAALRHCDDLVTEFLTRMSPLAPRIGQYWLQL
                     PATFGPRELPALWHFLDSLPGEFNYGVEVRHPQFFAKGEEEQTINRGLHQRGVNRVIL
                     DSRPIHAARPHSEAIRDAQRKKPKVPVHAVLTAKNPLIRFIGSDDMTQNRELFQVWLQ
                     KLAQWHQTTTPYLFLHTPDIAQAPELVHTLWEDLRKTLPEIGAVPAIPQQSSLF"
     gene            5875..6270
                     /locus_tag="J1Y83_003537"
     CDS             5875..6270
                     /locus_tag="J1Y83_003537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310606.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA7631652.1"
                     /translation="MVSALYAVLSALLLMKFSFDVVRLRMQYRVAYGDGGFSELQSAI
                     RIHGNAVEYIPIAIVLMLFMEMNGAETWMVHICGIVLLAGRLMHYYGFHHRLFRWRRS
                     GMSATWCALLLMVLANLWYMPWELVFSLR"
     gene            6311..7054
                     /gene="cmoA"
                     /locus_tag="J1Y83_003538"
     CDS             6311..7054
                     /gene="cmoA"
                     /locus_tag="J1Y83_003538"
                     /EC_number="2.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy-S-adenosyl-L-methionine synthase CmoA"
                     /protein_id="HBA7631653.1"
                     /translation="MSHRDTLFSAPIARLGDWTFDERVAEVFPDMIQRSVPGYSNIIS
                     MIGMLAERFVQPGTQVYDLGCSLGAATLSVRRNIHHDNCKIIAIDNSPAMIERCRRHI
                     DSYKAPTPVDVIEGDIRDIAIENASMVVLNFTLQFLEPSERQALLDKIYQGLNPGGAL
                     VLSEKFSFEDAKVGELLFNMHHDFKRANGYSELEISQKRSMLENVMLTDSVETHKARL
                     HKAGFEHSELWFQCFNFGSLVALKAEDAA"
     gene            7051..8022
                     /gene="cmoB"
                     /locus_tag="J1Y83_003539"
     CDS             7051..8022
                     /gene="cmoB"
                     /locus_tag="J1Y83_003539"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-methoxyuridine(34)/uridine 5-oxyacetic
                     acid(34) synthase CmoB"
                     /protein_id="HBA7631654.1"
                     /translation="MIDFGNFYSLIAKNHLSHWLETLPAQIANWQREQQHGLFKQWSN
                     AVEFLPEIKPYRLDLLHSVTAESEEPLSAGQIKRIETLMRNLMPWRKGPFSLYGVNID
                     TEWRSDWKWDRVLPHLSDLTGRTILDVGCGSGYHMWRMIGAGAHLAVGIDPTQLFLCQ
                     FEAVRKLLGNDQRAHLLPLGIEQLPALKAFDTVFSMGVLYHRRSPLEHLWQLKDQLVN
                     EGELVLETLVIDGDENTVLVPGDRYAQMRNVYFIPSALALKNWLKKCGFVDIRIADVS
                     VTTTEEQRRTEWMVTESLADFLDPHDPGKTVEGYPAPKRAVLIARKP"
     gene            complement(8187..10616)
                     /locus_tag="J1Y83_003540"
     CDS             complement(8187..10616)
                     /locus_tag="J1Y83_003540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310609.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin guanine dinucleotide-containing
                     S/N-oxide reductase"
                     /protein_id="HBA7631655.1"
                     /translation="MTLTRREFIKHSGIAAGTLVVTSAAPLPAWAEEKGGKILTAGRW
                     GAMNVEVKDGKIVSSTGALAKTIPNSLQSTAADQVHTTARIQHPMVRKSYLDNPLQPV
                     KGRGEDTYVQVSWEQALKLIHEQHDRIRKANGPSAIFAGSYGWRSSGVLHKAQTLLQR
                     YMNLAGGYSGHSGDYSTGAAQVIMPHVVGSVEVYEQQTSWPLILENSQVVVLWGMNPL
                     NTLKIAWSSTDEQGLEYFHQLKKSGKPVIAIDPIRSETIEFFGDNATWIAPNMGTDVA
                     LMLGIAHTLMTQGKHDKVFLEKYTTGYPQFEEYLTGKSDNTPKSAAWAAEITGVPEAQ
                     IVKLAELMAANRTMLMAGWGIQRQQYGEQKHWMLVTLAAMLGQIGTPGGGFGFSYHYS
                     NGGNPTRVGGVLPEMSAAIAGQASEAADDGGMTAIPVARIVDALENPGGKYQHNGKEQ
                     TYPNIKMIWWAGGGNFTHHQDTNRLIKAWQKPEMIVVSECYWTAAAKHADIVLPITTS
                     FERNDLTMTGDYSNQHIVPMKQAVAAQFEARNDFDVFADLAELLKPGGKEIYTEGKDE
                     MAWLKFFYDAAQKGARAQRVTMPMFNAFWQQNKLIEMRRSEKNEQYIRYGDFRADPVK
                     NALGTPSGKIEIYSRTLEKFGYKDCPAHPTWLAPDEWKGTADEKQLQLLTAHPAHRLH
                     SQLNYAELRKKYAVADREPITIHTEDAARFGIANGDLVRVWNKRGQILTGAVVTDGIK
                     KGVVCVHEGAWPDLENGLCKNGSANVLTADISSSQLANACAGNSALVYIEKYTGNALK
                     LTAFDQPAVQA"
     gene            complement(10641..11738)
                     /locus_tag="J1Y83_003541"
     CDS             complement(10641..11738)
                     /locus_tag="J1Y83_003541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome C"
                     /protein_id="HBA7631656.1"
                     /translation="MRGKKRIGLLFLLIAVVVGGGLLLAQKALHKTSDTAFCLSCHSM
                     SKPFEEYQGTVHFSNQKGIRAECADCHIPKSGMDYLFAKLKASKDIYHEFVSGKIDSD
                     DKFEAHRQEMAETVWKELKATDSATCRSCHSFDAMDIASQSESAQKMHNKAQKDGETC
                     IDCHKGIAHFPPEIKMDDNAAHELESQAATSVTNGAHIYPFKTSRIGELATVTPGTDL
                     TVVDASGKQPIVRLQGYQMQGSENTLYLAAGQRLALATLSEEGIKALTVNGEWQADEY
                     GNQWRQASLQGALIDPALADRKPLWQYAEKLDDTYCAGCHAPIAADHYTVNTWPSIAK
                     GMGARTSMSENELDILTRYFQYNAKDITEKQ"
BASE COUNT         2694 a         3145 c         2907 g         3089 t
ORIGIN      
        1 ctgctgaata tgaaattggt gatatcgtct ttacatgtat aggtgctgcc ttatttggtc
       61 aaatatcagc tgcatcaaat tgctggagta atcacgtcgg gatcattatc ggtcataacg
      121 gtgaagactt tctggttgca gaaagccgtg ttcccctctc aaccatcact acgctatccc
      181 gttttattaa acgctctgct aatcaacgct atgctataaa gcgattagac gccggactaa
      241 cagaacaaca aaaacaacga attgttgaac aggttccttc ccggctacgc aaactttacc
      301 acaccggttt taaatacgaa tcttcgcgcc agttctgttc aaaatttgtt tttgatattt
      361 ataaagaggc gctatgtatt ccggtgggtg aaatagagac gtttggagaa ttgttaaata
      421 gcaatccgaa tgcaaaactc actttctgga aattctggtt cttaggttct attccgtggg
      481 agcgtaaaac cgtcacgcca gccagtttgt ggcatcatcc gggtttggtg ttgattcacg
      541 cggtgggagt tgaaacgcct cagcctgaac tgaccgaggc ggtataactt aacgcagtcg
      601 ccctctcgcc aggttcagtc gcgattcgct catttgcatc gcattctgac taacgtggca
      661 gtgggtgatg gcaatcgcca gcgcatcggc ggcatccgcc tgtggattag cgggcagttt
      721 cagcaaggtg cggaccatat gctgcacctg gcttttttcg gcactaccaa tacctaccac
      781 tgtttgcttt acctgacgtg ccgcatattc aaataccggc aattcctgat tcaccgccgc
      841 cacaatcgcc acgccgcgcg cctgccccag tttcagggct gagtcagcgt tcttcgccat
      901 aaagacctgt tcaatggcga aataatcagg ctggaattgg gtgatgattt ccgtcacgcc
      961 cgcatagatg agcttcagac gagacggtaa atcatccact ttggtgcgta tgcatccgct
     1021 acccaggtag gacagttgcc tgcctacctg gcggatgacg ccatagccgg tcacgcgcga
     1081 acccgggtca atgccgagaa taatagccat cacgcgtctc cgttttgctg tttagcaggc
     1141 ctcatcagag agtcgctgca acctcatcag agatttcacc gttatggtaa acttcctgca
     1201 cgtcgtcgca atcttccagc atatcgatca gacgcatcag tttcggtgcg gtttctgcat
     1261 ccatatcagc tttggtggac gggatcatgg aaacttccgc gctgtctgct ttcagacctg
     1321 ccgcttccag agcgtcgcgt actttgccca tttcttccca tgcagtgtag acatcaatcg
     1381 cgccgtcatc ataggtcaca acgtcttcag caccggcttc cagggctgct tccatgatgg
     1441 tgtcttcatc gcctttctcg aaggagatca cgcctttttt gctgaacaaa taagctacgg
     1501 aaccatcagt accgaggtta ccgccacatt tgctgaatgc atgacgcact tcagcaacgg
     1561 tacggttgcg gttgtcagac agacattcaa tcatgattgc cgtgccgcca ggaccgtaac
     1621 cttcgtagat gatggtttcc atgtttgcat catcatcacc gcccacacct cgtgcaattg
     1681 cgcggttcag agtgtcacgg gtcatgttgt tagacagtgc tttatcaatt gctgcacgca
     1741 aacgcgggtt agcgtccgga tcaccaccgc ccagcttagc cgcggttacc agctcacgaa
     1801 tgattttagt gaagatttta ccgcgcttag catcctgcgc agctttacga tgtctggtgt
     1861 tggcccattt actatgacct gccataaaaa tatctccaga tagccctgcc tgttcaggca
     1921 gcgttaatta caaactgttc aatcgcctgc cggttgctcc aggacttagt gagcgccgcc
     1981 gcagcagacg catcaagcca cttgtaagcc agatgttcag tgaaaacgat ctggcgctcg
     2041 tgcgggagcg caagacagaa ccatgattcc gtattacgcg tcacgcccgg cgcatagcga
     2101 tgacgtaaat gtgaaaaaat ttcaaactct accgtgcgct gacagtcaat taaggtcagt
     2161 tgttcagcga caacatcaat ggtgacctct tcctttactt cgcgcatggc agcttgcggc
     2221 gcggtttccc cctcttccac gctgccggtt accgactgcc agaaatcggg atcgtcacgc
     2281 cgctgcaaca tcagcacccg tttcgtatct tgtgcgtaga tgaccactaa gatcgaaacg
     2341 ggacgcttat aagccatatc agttattctc agccttcttc acaacctgaa tgctcagctc
     2401 agccagtgca gtcgggttag caaagctcgg tgcttcagtc atcagacacg ctgccgccgt
     2461 ggttttcggg aaggcgataa cgtcacgtat attgtcggtg ccggtcagca gcatggtcag
     2521 acggtcaaga ccgaatgcca gacctgcgtg cggcggagta ccgtatttca gggcgtcgag
     2581 caggaagccg aatttctcgc gctgttcctc ttcgttgata cccagaatac caaacaccgt
     2641 ctgctgcata tcaccattat ggatacgcac agaaccaccg cccacttcgt aaccattgat
     2701 gaccatatcg taagcgttcg ccaccgcatt ttccggtgca gctttcagtt ctgctgccgt
     2761 catgtctttc ggtgaggtga acggatggtg cattgccgtc aggccgcctt caccgtcgtc
     2821 ttcaaacatc gggaagtcga taacccacag cggtgcccat ttgctttcgt cggtcagacc
     2881 aaggtcttta cccactttca ggcgcagtgc ccccatcgcg tcggcaacaa ttttcttgtt
     2941 gtcggcaccg aagaaaatca tatcgccatc ttgcgcgcca gtacgctcca ggatggcttc
     3001 aatgatttct gcattgagga acttcgctac cgggctgttg ataccttcca gacctttcgc
     3061 gcgttcgtta actttgatgt aagccagacc tttcgcgccg tagattttaa cgaagttacc
     3121 gtattcgtcg atctgcttac gggtcaacga tgcgccgccc ggaacacgca gagcggcaac
     3181 acggcctttc ggatcgttcg ccggacctgc aaatactgca aactcaacag atttcagcag
     3241 atcggcaacg tcggtcagtt ccatcgggtt acgcagatcc ggtttatcag aaccataacg
     3301 gcgttctgct tctgcaaagg tcattaccgg gaaatcgccc agatccacgc ccttcacttc
     3361 cagccacaga tgacgcacca gcgcttccat cacttcacgc acttgcggcg cggtcatgaa
     3421 agaagtttcc acatcgatct gagtaaattc aggctgacgg tcagcacgca ggtcttcgtc
     3481 acggaagcat ttaacgatct gatagtagcg gtcaaagccg gacatcatca gtagctgttt
     3541 gaacaactgc ggggattgcg gcagcgcgta gaatttacct ttgtgcacac gagaaggcac
     3601 caggtagtca cgcgcgcctt ccggcgtggc tttggtcagc atcggagttt cgatgtcgag
     3661 gaagccgtgg tcatccataa aacggcgcac caggctggtg attttagcgc gggttttcag
     3721 gcgctgagcc atttccgggc gacgcaggtc gaggtagcgg tatttcagac gcgcttcttc
     3781 ggtgttgacg tggttagagt caagcggcag aacatctgca cggttgatga tagtcagcga
     3841 ggacgccagt acttcgattt cgccagtcgc catatcgcgg ttaatatttt tttcgtcacg
     3901 cgcacgtacg gtgcccgtga cctgaatgca gaactcatta cgcagttcag aggccagctt
     3961 taacgcgtcc gcacgatccg gatcgaaaaa tacctgcacg ataccttcgc ggtcgcgcat
     4021 atcgatgaag atcaggctac caagatcacg acgacggttg acccaaccac acagagtcac
     4081 ctgctgcccc acgtgggaca aacggagctg tccacaatat tctgtacgca tgagatatcc
     4141 cttaacttag ctgccggcgg atgccccctg ctgcgcaggt gaccaagtcg cagcgttagc
     4201 tgtatgtcac aactgaatga aaaaaggcgg ctattatact ggaaattctg ccgcaccgta
     4261 agagcctggc ccgcgctgga acgcctcgtt accactttat atcgggcctg aaatcagact
     4321 ctacgccagt ttgctataaa ggtgttgccc gaactcataa aaattaacaa aatttgtcgt
     4381 tccgccatcg gctaatcgca ttaaggtgag aggcacgatt ttgttttatc aggagtcatc
     4441 atgcttgaac ttaatgctaa aaccaccgcg ctggtggtga ttgatttaca agaaggcatc
     4501 ttgccttttg ccggaggtcc acatactgcc gatgaggtgg ttaatcgggc agggaagctg
     4561 gcggcgaaat ttcgcgcgaa cggtcagccc gtgtttctgg tgcgcgttgg ctggtctgcc
     4621 gattacgccg aagcattaaa acagccggtt gatgccccct cccccgcaaa agtgttgccc
     4681 gaaaactggt ggcaacatcc tgctgcatta ggtgcaaccg acagcgatat cgaaatcatc
     4741 aaacgtcaat ggggtgcgtt ttacggtacg gatctggagt tgcaattacg ccgccggggt
     4801 atcgatacaa tagtgttatg tgggatctcg accaatatcg gtgttgaatc caccgcccgc
     4861 aatgcctggg aactcggttt taatctggtg attgccgaag atgcctgtag cgccgctagc
     4921 gccgagcagc acaataacag cattaatcat atctacccgc gcatcgcccg tgtgcgtagc
     4981 gttgaagaga tcctccacgc gttatgattt acatcggttt gccacaatgg tcgcatccta
     5041 aatgggtgcg gttggggatc accagccttg aagagtatgc ccgccacttt aactgtgtgg
     5101 agggtaacac cacgctttat gccctgccga aacccgaggt tgtcctgcgc tggcgtgagc
     5161 agaccacaga tgacttccgc ttctgtttta agtttccggc gactatttcg catcaggcag
     5221 cattacggca ttgcgatgat ttagtgactg aatttttgac ccgcatgtca ccgttggctc
     5281 cgcgcattgg acaatactgg ctgcaactgc ctgccacatt cggcccacgg gagctgcctg
     5341 cgctttggca ttttctcgac tctcttcccg gcgaatttaa ttatggtgtg gaagtccgcc
     5401 atccacagtt tttcgccaaa ggggaagagg aacaaacgat taatcgcggt ttacatcagc
     5461 gcggcgttaa tcgggtgatt ttagacagcc gcccgattca tgcagcacgt ccacacagtg
     5521 aagctattcg cgacgctcaa cgaaaaaaac ctaaagtgcc ggtacatgct gtactgacgg
     5581 cgaaaaatcc actgatccgt tttatcggta gtgatgatat gacgcaaaac cgggaattat
     5641 ttcaggtctg gttacaaaaa ttagcgcagt ggcatcagac cactacgcct tatctttttt
     5701 tacatacgcc agatattgcc caggccccgg aactggtaca taccctgtgg gaagacttac
     5761 gtaaaacgct tccagagatc ggagcagttc cggctattcc acagcaatct tctcttttct
     5821 gaatttgcca cctatcatag acaggtgcca tcggccattt taaagggagt ttgtatggta
     5881 agcgcgctgt atgccgtttt aagtgcgttg ttattaatga agttctcttt tgatgtcgtt
     5941 cgcctgcgaa tgcagtaccg cgttgcctat ggcgacggcg gttttagcga actgcaaagc
     6001 gctattcgca ttcatggtaa cgcggtggaa tatattccta tcgcgattgt gttgatgctg
     6061 tttatggaaa tgaatggcgc agaaacctgg atggtgcata tttgcggcat cgttttgctt
     6121 gctggtcgtc tgatgcatta ttacggtttt catcaccgtc tgttccgctg gcgacgttct
     6181 ggcatgagcg cgacctggtg tgcgctgttg ctgatggtgc tggcgaatct ttggtatatg
     6241 ccctgggagt tggttttctc cctgcgttag cgcacaatac gccactttct ttttcccgga
     6301 tttttacgtt atgtctcacc gcgacacgct attttctgcc cctatcgcca gactgggcga
     6361 ctggaccttt gatgaacggg tagctgaagt cttcccggat atgatccagc gttcagttcc
     6421 cggctattcc aatattattt ccatgattgg tatgttagcc gagcgcttcg ttcaacctgg
     6481 tacgcaggtt tacgatctgg gttgttctct gggcgcggcg acgctctcgg tgcgtcgcaa
     6541 cattcatcat gataattgca aaattattgc catcgacaac tccccggcga tgattgaacg
     6601 ctgccgtcgt catattgact cctataaagc tcctacgcca gtagacgtta ttgaaggtga
     6661 tattcgcgat atcgccattg aaaacgcatc gatggtggtg ctgaatttta ccctgcaatt
     6721 cctggaacct tccgagcgcc aggcgttact ggataaaatt tatcaagggc tgaacccggg
     6781 cggtgcgctg gtgctttcgg aaaaattcag tttcgaagat gccaaagttg gtgaattgct
     6841 gttcaacatg caccacgact ttaaacgtgc caacggttac agcgaactgg agatcagcca
     6901 gaaacgcagc atgctggaaa acgtgatgct gaccgattcc gtggaaaccc ataaagcacg
     6961 cctgcataaa gccggttttg agcatagcga gctgtggttc cagtgcttta actttggttc
     7021 actggtggca ttaaaagcag aggacgctgc atgatcgact ttggtaactt ttattctctg
     7081 attgccaaaa atcatctttc acactggctc gaaacgctgc ccgcgcagat tgctaactgg
     7141 cagcgcgagc agcagcacgg gctgtttaag cagtggtcca acgcggtgga atttctgcct
     7201 gaaattaaac cgtatcgtct tgatttactg catagcgtga cggcagaaag cgaagaacca
     7261 ctgagcgccg ggcaaattaa acgcattgaa acgctgatgc gcaacctgat gccgtggcgc
     7321 aaagggccgt tctcactgta tggcgtcaac atcgataccg aatggcgttc cgattggaaa
     7381 tgggatcgcg ttctgcccca tctttctgat ttaaccgggc gcaccattct tgatgttggc
     7441 tgcggcagcg gttatcacat gtggcgcatg attggcgcag gcgcgcacct cgcggtgggg
     7501 atcgacccta cgcaactgtt tctgtgccag tttgaagcgg tgcgtaaact actgggcaac
     7561 gatcagcgcg cacatttgtt accgttaggt attgaacaac ttccggcact gaaagccttt
     7621 gataccgtct tttcgatggg cgtgctttat catcgtcgtt caccgctgga gcatctctgg
     7681 cagttaaaag accaactggt gaatgaaggc gaactggtgc tggaaacgct ggttattgat
     7741 ggcgatgaga acacggtgct ggtgccgggc gatcgttacg cacaaatgcg taatgtctat
     7801 ttcattcctt ccgcgctggc gctgaaaaac tggctgaaga agtgtggttt tgttgatatt
     7861 cgcattgcag atgtgagcgt taccaccaca gaagagcagc gacgcaccga atggatggtc
     7921 accgagtctc tggccgattt tctcgacccg catgatccgg gtaagacagt ggaaggttat
     7981 cctgcgccta aacgcgcggt gctgattgcg cgcaagccgt aaaggtctgg taatactgcc
     8041 ggatgcggcg tgaacgcctt atccggccta caaagtcttg ctaattcaat atattgcagg
     8101 ggctatgtag gcctgataag catagcgcat caggcagttt tgcgtttgca taacctcagc
     8161 gcccgtttcc gggcgctttt cacgtcttat gcctgaacag ctggctgatc aaacgccgtt
     8221 aacttcagcg cattgcccgt atatttttcg atatacacca gcgcagagtt accggcacag
     8281 gcattcgcca gctgcgagct ggagatatcc gccgttaaca cgttcgcact accgttttta
     8341 cacaagccat tttccagatc cggccatgca ccttcatgca cgcataccac gccttttttg
     8401 atcccgtcag tcaccacagc gcctgtcagg atctgaccgc gcttgttcca gacacgcacc
     8461 agatcgccat tcgcaatacc aaagcgagca gcatcttcgg tgtgaatagt gattggttca
     8521 cgatcggcaa ccgcatattt tttacgcagt tccgcatagt taagctgact gtgtaaacgg
     8581 tgtgccggat gtgcggtcag aagctgcaac tgcttctcgt cggcggtacc cttccactca
     8641 tcaggcgcaa gccaggttgg gtgtgccggg caatccttat agccaaattt ttccagcgtt
     8701 ctggagtaaa tctcaatttt gccgcttggt gtacccagcg catttttcac cggatcggcg
     8761 cggaaatcac cataacgaat gtactgttcg ttcttctcgc tgcggcgcat ttcgatcagt
     8821 ttattttgct gccagaaggc attaaacatc ggcatagtca cgcgttgcgc acgggcacct
     8881 ttctgagcgg catcatagaa aaatttcagc cacgccattt catctttacc ttcggtatag
     8941 atctcttttc cgccaggttt gagtaattca gcaagatcgg caaacacgtc aaaatcgttg
     9001 cgcgcttcaa attgcgcagc gacagcctgc ttcatcggca caatatgctg gttgctgtaa
     9061 tcaccggtca tcgtcaggtc attgcgctca aacgatgtgg tgatcggtaa tacgatatct
     9121 gcgtgtttgg ctgctgcggt ccagtagcat tcagaaacga cgatcatctc cggtttctgc
     9181 catgctttaa tcagacgatt agtatcctgg tgatgggtaa agttaccgcc acccgcccac
     9241 cagatcattt tgatatttgg gtacgtttgc tctttaccgt tatgctgata tttgccgcca
     9301 ggattttcca gcgcatcgac gatacgggca acaggaatcg ccgtcattcc gccatcatca
     9361 gcggcctcgc tggcctgtcc ggcaatcgcc gcagacattt ctggtagcac gccgccaaca
     9421 cgcgtcgggt tgccaccgtt ggagtaatga taagagaaac caaacccgcc gcccggtgta
     9481 ccaatctgcc ctaacatcgc cgccagcgtc accagcatcc agtgtttctg ttcaccatat
     9541 tgctggcgct gaattcccca gcctgccatc agcattgtgc gatttgccgc catcagttca
     9601 gcgagtttga ctatctgcgc ttccggtacg ccagtaattt ccgccgccca ggccgcgctc
     9661 ttcggcgtat tatcgctctt acctgtcaga tactcttcaa actgcggata cccggtagtg
     9721 tatttttcaa ggaacacttt atcgtgtttg ccttgtgtca tcagggtatg cgcaatccct
     9781 aacatcagtg ccacatcggt gcccatattc ggtgcgatcc aggtggcatt atcgccaaag
     9841 aattcgatgg tttcggagcg gataggatca atggcaatca ctggtttgcc agattttttc
     9901 agctgatgga agtattccag cccttgctca tcggtactgc tccaggcaat tttgagcgtg
     9961 ttcagcgggt tcatccccca cagcaccacc acctggctgt tttccagaat aagcggccag
    10021 gaggtctgct gttcatacac ctctacagaa ccgaccacat gcggcatgat cacctgtgcc
    10081 gctccggttg aataatcgcc gctatgcccg gaataaccgc ccgccaggtt catataacgt
    10141 tgcagtaagg tttgcgcttt atgcaacacg ccagaagaac gccagccata agatccggca
    10201 aaaatggccg atggtccgtt agctttgcga atgcgatcgt gttgttcatg aatcagtttt
    10261 aatgcttgtt cccagctcac ctgtacatag gtatcttcgc cacgaccttt caccggttgc
    10321 agtggattat cgagatagct ttttctcacc atcggatgct gaatacgcgc cgtggtgtgt
    10381 acctgatccg ccgccgtaga ctgtaaggag ttcggtatgg ttttcgccag cgcgcctgtc
    10441 gaagaaacaa tcttgccgtc cttcacttct acgttcatcg ctccccaacg gcccgcagtg
    10501 agaattttgc cgcccttctc ttctgcccat gcgggtagcg gtgcagccga tgtcaccacc
    10561 agcgtccctg ccgcaatacc gctatgttta ataaattcac gtcttgttaa tgtcataact
    10621 tcctccctga tcaacgagga tcactgtttc tcggtaatat ctttggcgtt gtactggaag
    10681 taccgcgtca aaatgtccag ttcgttttcg ctcatgctgg ttcgcgcacc cattcctttg
    10741 gcaatggacg gccatgtatt gacggtgtaa tggtcggcgg caataggggc atggcagcca
    10801 gcgcaatagg tatcgtcaag tttttcagcg tattgccata gcggtttacg gtccgctaat
    10861 gcgggatcga taagcgcccc ctgcaaagac gcctgacgcc attgattgcc gtattcgtca
    10921 gcctgccatt ccccgtttac cgttagcgcc ttgatacctt cttcacttaa tgtggcgagc
    10981 gccagccgtt ggcctgccgc caggtagagc gtgttttcac tgccctgcat ttgataaccc
    11041 tgcaaccgaa caattggctg tttgccactg gcatcaacga cggtaagatc ggtaccagga
    11101 gtcacggtag ccagttcgcc tatgcgagaa gttttgaaag gataaatatg tgcgccatta
    11161 gtaactgaag tagcggcctg actttccagc tcatgcgccg cgttgtcatc catttttatt
    11221 tctggcggaa aatgggcaat gcctttatgg caatcgatac aggtttcgcc gtccttttgc
    11281 gctttattat gcattttctg cgcagattca ctttgcgagg caatatccat ggcatcaaaa
    11341 gaatggcaac tacggcaggt tgcagagtca gttgctttta attctttcca tactgtttcg
    11401 gccatttcct ggcgatgagc ttcgaactta tcgtcactgt ctattttgcc gctaacaaat
    11461 tcatgataaa tatctttaga tgcttttaat ttagcaaata aataatccat ccctgacttt
    11521 ggaatatggc aatcggcaca ttccgcacgt atccctttct ggtttgaaaa gtggacagtt
    11581 ccctgatatt cctcaaaagg tttactcatc gagtggcagg aaaggcaaaa tgcggtatcc
    11641 gacgttttat gtaaggcttt ttgcgccagt aataacccgc caccaaccac aaccgctatc
    11701 agcaaaaaca ataacccaat gcgttttttc cctcgcatag attcctctaa ttaaattcca
    11761 ggacaccgat accattgtat aaataaatac acaacgaatt gactacttgc ttaattcggc
    11821 acaacactgc ctgaa
//
LOCUS       DADSYM010000142        11726 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2415
            SAMN08039579-rid11723173.denovo.143, whole genome shotgun sequence.
ACCESSION   DADSYM010000142 DADSYM010000000
VERSION     DADSYM010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039579
            Sequence Read Archive: SRR6362533
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11726)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11726)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980162.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 03:39:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,484
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 4,347
            CDSs (with protein)               :: 4,347
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 43 of 85
            Pseudo Genes (incomplete)         :: 37 of 85
            Pseudo Genes (internal stop)      :: 19 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11726
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039579-rid11723173.denovo.143"
                     /strain="LGE2415"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8302 N 95.9628 W"
                     /collection_date="2015-08-24"
     gene            complement(55..1395)
                     /gene="lamB"
                     /locus_tag="J1Y83_003542"
     CDS             complement(55..1395)
                     /gene="lamB"
                     /locus_tag="J1Y83_003542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltoporin LamB"
                     /protein_id="HBA7631657.1"
                     /translation="MMITLRKLPLAVAVAAGVMSAQAMAVDFHGYARSGIGWTGSGGE
                     QQCFQTTGAQSKYRLGNECETYAELKLGQEVWKEGDKSFYFDTNVAYSVAQQNDWEAT
                     DPAFREANVQGKNLIEWLPGSTIWAGKRFYQRHDVHMIDFYYWDISGPGAGLENIDVG
                     FGKLSLAATRSSEAGGSSSFASNNIYDYTNETANDVFDVRLAQMEINPGGTLELGVDY
                     GRANLRDNYRLVDGASKDGWLFTAEHTQSVLKGFNKFVVQYATDSMTSQGKGLSQGSG
                     VAFDNEKFAYNINNNGHMLRILDHGAISMGDNWDMMYVGMYQDINWDNDNGTKWWTVG
                     IRPMYKWTPIMSTVMEIGYDNVESQRTGDKNNQYKITLAQQWQAGDSIWSRPAIRVFA
                     TYAKWDEKWGYDYTGSSSTNPYYGKAVSADFNGGSFGRGDSDEWTFGAQMEIWW"
     gene            complement(1467..2582)
                     /gene="malK"
                     /locus_tag="J1Y83_003543"
     CDS             complement(1467..2582)
                     /gene="malK"
                     /locus_tag="J1Y83_003543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011915502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose/maltodextrin ABC transporter ATP-binding
                     protein MalK"
                     /protein_id="HBA7631658.1"
                     /translation="MASVQLQNVTKAWGEVVVSKDINLDIHEGEFVVFVGPSGCGKST
                     LLRMIAGLETITSGDLFIGEKRMNDTPPAERGVGMVFQSYALYPHLSVAENMSFGLKL
                     AGAKKEVINQRVNQVAEVLQLAHLLDRKPKALSGGQRQRVAIGRTLVAEPSVFLLDEP
                     LSNLDAALRVQMRIEISRLHKRLGRTMIYVTHDQVEAMTLADKIVVLDAGRVAQVGKP
                     LELYHYPADRFVAGFIGSPKMNFLPVKVTATAIDQVQVELPMPNRQQVWLPVESRDVQ
                     VGANMSLGIRPEHLLPSDIADVILEGEVQVVEQLGNETQIHIQIPSIRQNLVYRQNDV
                     VLVEEGATFAIGLPPERCHLFREDGTACRRLHKEPGV"
     gene            2947..4137
                     /gene="malE"
                     /locus_tag="J1Y83_003544"
     CDS             2947..4137
                     /gene="malE"
                     /locus_tag="J1Y83_003544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000695396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose/maltodextrin ABC transporter
                     substrate-binding protein MalE"
                     /protein_id="HBA7631659.1"
                     /translation="MKIKTGARILALSALTTMMFSASALAKIEEGKLVIWINGDKGYN
                     GLAEVGKKFEKDTGIKVTVEHPDKLEEKFPQVAATGDGPDIIFWAHDRFGGYAQSGLL
                     AEITPDKAFQDKLYPFTWDAVRYNGKLIAYPIAVEALSLIYNKDLLPNPPKTWEEIPA
                     LDKELKAKGKSALMFNLQEPYFTWPLIAADGGYAFKYENGKYDIKDVGVDNAGAKAGL
                     TFLVDLIKNKHMNADTDYSIAEAAFNKGETAMTINGPWAWSNIDTSKVNYGVTVLPTF
                     KGQPSKPFVGVLSAGINAASPNKELAKEFLENYLLTDEGLEAVNKDKPLGAVALKSYQ
                     DELAKDPRIAATMDNAQKGEIMPNIPQMSAFWYAVRTAVINAASGRQTVDAALKDAQT
                     RITK"
     gene            4291..5835
                     /gene="malF"
                     /locus_tag="J1Y83_003545"
     CDS             4291..5835
                     /gene="malF"
                     /locus_tag="J1Y83_003545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose ABC transporter permease MalF"
                     /protein_id="HBA7631660.1"
                     /translation="MDVIKKKHWWQSDALKWSVLGLLGLLVGYLVVLMYAQGEYLFAI
                     TTLILSSAGLYIFANRKAYAWRYVYPGMAGMGLFVLFPLVCTIAIAFTNYSSTNQLTF
                     ERAQEVLLDRSWQAGKIYNFGLYPAGDEWQLALSDGETGKNYLSDAFKFGGEQKLQLK
                     ESATQPEGERANLRVITQNRQTLSDITAILPDGNKVMMSSLRQFSGTQPLYTLDGDGT
                     LTNNQSGVKYRPNDQIGFYQSITADGNWGDEKLSPGYTVTTGWKNFTRVFTDEGIQKP
                     FLAIFVWTVVFSLITVFLTVAVGMVLACLVQWEALRGKAVYRVLLILPYAVPSFISIL
                     IFKGLFNQSFGEINMMLSALFGVKPAWFSDPTTARTMLIIVNTWLGYPYMMILCMGLL
                     KAIPDDLYEASAMDGAGPFQNFFKITLPLLIKPLTPLMIASFAFNFNNFVLIQLLTNG
                     GPDRLGTTTPAGYTDLLVNYTYRIAFEGGGGQDFGLAAAIATLIFLLVGALAIVNLKA
                     TRMKFD"
     gene            5850..6740
                     /gene="malG"
                     /locus_tag="J1Y83_003546"
     CDS             5850..6740
                     /gene="malG"
                     /locus_tag="J1Y83_003546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001252048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose ABC transporter permease MalG"
                     /protein_id="HBA7631661.1"
                     /translation="MAMVQPKSQKARLFITHLLLLLFIAAIMFPLLMVVAISLRQGNF
                     ATGSLIPEQISWDHWKLALGFSVEQADGRITPPPFPVLLWLWNSVKVAGISAIGIVAL
                     STTCAYAFARMRFPGKATLLKGMLIFQMFPAVLSLVALYALFDRLGEYIPFIGLNTHG
                     GVIFAYLGGIALHVWTIKGYFETIDSSLEEAAALDGATPWQAFRLVLLPLSVPILAVV
                     FILSFIAAITEVPVASLLLRDVNSYTLAVGMQQYLNPQNYLWGDFAAAAVMSALPITI
                     VFLLAQRWLVNGLTAGGVKG"
     gene            complement(6834..7244)
                     /gene="psiE"
                     /locus_tag="J1Y83_003547"
     CDS             complement(6834..7244)
                     /gene="psiE"
                     /locus_tag="J1Y83_003547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate-starvation-inducible protein PsiE"
                     /protein_id="HBA7631662.1"
                     /translation="MTSLSRPRVEFISTILQTVLNLGLLCLGLILVVFLGKETVHLAD
                     VLFAPEQTSKYELVEGLVVYFLYFEFIALIVKYFQSGFHFPLRYFVYIGITAIVRLII
                     VDHKSPLDVLIYSAAILLLVITLWLCNSKRLKRE"
     gene            7459..7737
                     /locus_tag="J1Y83_003548"
     CDS             7459..7737
                     /locus_tag="J1Y83_003548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA7631663.1"
                     /translation="MKRNLIKEVKMKPYFAALMLSVSVLPAYAGPLGTADKADLPQSN
                     VSSPMMAQSLRQPDLQPISTDRKTECFRLYTPDRKPGVNCVPDGSTGH"
     gene            complement(7784..9880)
                     /locus_tag="J1Y83_003549"
     CDS             complement(7784..9880)
                     /locus_tag="J1Y83_003549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbH domain-containing protein"
                     /protein_id="HBA7631664.1"
                     /translation="MKKRHLLSLLALGISTACYGETYPAPIGPSQSDFGGVGLLQTPT
                     ARMAREGELSLNYRDNDQYRYYSASVQLFPWLETTLRYTDVRTRQYSSVEAFSGDQTY
                     KDKAFDLKLRLWEESYWLPQVAVGARDIGGTGLFDAEYLVASKAWGPFDFTLGLGWGY
                     LGTSGNVKNPLCSASDKYCYRDNSYKQAGSIDGSQMFHGPASLFGGVEYQTPWQPLRL
                     KLEYEGNNYQQDFAGKLEQKSKFNIGAIYRVTDWADVNLSYERGNTFMFGVTLRTNFN
                     DLRPSYNDNARPQYQPQPQDAILQHSVVANQLTLLKYNAGLADPQIQTKGDTLYVTGE
                     QVKYRDPREGIIRANRIVMNDLPDGIKTIRITENRLNMPQVTTETDVASLKNHLAGEP
                     LGHETKLVQKRVEPVVPKSTEQGWYIDKSRFDFHIDPVLNQSVGGPENFYMYQLGVMG
                     TADLWLTDHLLTTGSLFANLANNYDKFNYTNPPQDSHLPRVRTHVREYVQNDVYVNNL
                     QANYFQHLGNGFYGQVYGGYLETMFGGVGAEVLYRPLDSNWAFGLDANYVKQRDWRSA
                     KDMMKFTDYSVKTGHLTAYWTPSFAQDVLVKASVGQYLAGDKGGTLEIAKRFDSGVVV
                     GGYATITNVSKEEYGEGDFTKGVYVSVPLDLFSSGPTRSRAAIGWTPLTRDGGQQLGR
                     KFQLYDMTSDRSVNFR"
     gene            complement(9880..10617)
                     /locus_tag="J1Y83_003550"
     CDS             complement(9880..10617)
                     /locus_tag="J1Y83_003550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule biosynthesis GfcC family protein"
                     /protein_id="HBA7631665.1"
                     /translation="MIKQTIVALLLSAGASSVFAAGTVKVFSNGSSEAKTLTGAEHLI
                     DLVGQPRLANSWWPGAVISEELATAAALRQQQALLTRLAELAADSSADDAAAINALRQ
                     QIQALKVTGRQKINLDPDIVRVAERGNPPLQGNYTLWVGTPPSTVTLFGLISRPGKQS
                     FTPGRDVASYLSDQSLLSGADRSYAWVVYPDGRTQKAPVAYWNKRHVEPMPGSIIYVG
                     LADSVWSETPDALNADILQTLTQRIPQ"
     gene            complement(10614..11252)
                     /locus_tag="J1Y83_003551"
     CDS             complement(10614..11252)
                     /locus_tag="J1Y83_003551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418451.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbF family lipoprotein"
                     /protein_id="HBA7631666.1"
                     /translation="MKRPALILICLLLQACSATTKELGNSLWDSLFGTPGVQLTDDDI
                     QNMPYASQYMQLNGGPQLFVVLAFAEDGQQKWVTQDQATLVTQHGRLVKTLLGGDNLI
                     EVNNLATDPLIKPAQIIDGATWTRTMGWTEYQQVRYATARSVFKWDGIDTVKVGSDET
                     PVRVLDEEVSTDQARWHNRYWIDSEGQIRQSEQYLGADYFPVKTTLIKAAKQ"
     gene            complement(11367..11609)
                     /locus_tag="J1Y83_003552"
     CDS             complement(11367..11609)
                     /locus_tag="J1Y83_003552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA7631667.1"
                     /translation="MKKVLYGIFAISALAATSAWAAPVQVGEAAGSAATSVSAGSSSA
                     TSVSTVSSAVGVALAATGGGDGSNTGTTTTTTTSTQ"
BASE COUNT         2719 a         3157 c         3040 g         2810 t
ORIGIN      
        1 aggcattggt tgccggatgc ggcgcaagca ccttatccgg cccaggtttt gctattacca
       61 ccagatttcc atctgggcac cgaaggtcca ctcgtcgctg tcgccacgac cgaagctacc
      121 gccgttgaaa tcagccgaca cagccttgcc gtagtatggg ttggttgaag agctaccggt
      181 gtagtcataa ccccatttct catcccactt ggcgtaggtt gcgaagacac gaatagccgg
      241 gcgtgaccag atgctgtcgc cagcctgcca ttgttgtgca agggtaattt tgtactgatt
      301 gttcttgtcg ccggtgcgct gggattcgac gttgtcgtag ccgatttcca tcacggtgct
      361 catgattggc gtccacttgt acatcgggcg aataccgacg gtccaccact tggtgccgtt
      421 gtcgttatcc cagttgatat cctggtacat acccacgtac atcatgtccc agttgtcgcc
      481 catggagatc gcaccgtggt cgaggatacg cagcatgtga ccgttgttat tgatattgta
      541 ggcaaatttt tcgttatcaa acgcgacgcc agaaccctgc gacagacctt taccctgcga
      601 ggtcatcgag tcagtagcgt actgaacaac aaacttgtta aaacccttca ggacactctg
      661 agtatgttca gcagtgaata accagccgtc tttcgatgcg ccatcaacca gacgatagtt
      721 atcacgcagg ttggcacgac cgtagtcgac acccagttct aatgtgccgc ccgggttgat
      781 ttccatctgc gctaaacgca catcgaaaac gtcgttcgcg gtttcgttgg tatagtcata
      841 aatattgttg ctggcgaaag aggaagaacc acccgcttca gaggagcggg ttgctgccag
      901 agagagttta ccgaagccaa catcgatgtt ttccagaccg gcaccaggac cagaaatatc
      961 ccagtagtag aagtcgatca tatgaacgtc atgacgttgg tagaagcgct tacctgccca
     1021 gatggtggag cctggcagcc attcgatcag gtttttaccc tgcacgtttg cttcacggaa
     1081 ggccggatcg gtagcttccc agtcattctg ttgcgcgacg gaataggcca cgttagtgtc
     1141 gaaatagaag ctcttatcgc cctctttcca cacttcctga cccaatttta attcagcata
     1201 agtttcacat tcgttgccaa gacggtattt actttgagca ccggtagtct ggaaacactg
     1261 ttgttcgccg ccgctacctg tccagccaat accggaacgt gcatagccgt ggaaatcaac
     1321 agccattgcc tgagcagaca ttacgcccgc tgcgacggca accgccagag gaagtttgcg
     1381 cagagtaatc atcattctat ctcctgagtc attgcttttc ttttttcaca tcacctgtga
     1441 caggctttgt gtgttttgtg gggtgcttaa acgcccggct ccttatgcag tcgacgacat
     1501 gcagtgccat cctcacggaa cagatggcaa cgctctggcg gcaggccgat agcgaatgtg
     1561 gcaccttctt ctaccaacac cacgtcgttc tggcggtaca ccaggttttg acgaatggaa
     1621 gggatctgga tatggatttg ggtttcgttg ccgagttgct cgacgacctg aacttcaccc
     1681 tcaaggatga cgtcagcgat atcactcggc agtagatgtt ccgggcgaat acccagtgac
     1741 atattggctc caacctggac atcacggctt tcgaccggta gccagacttg ctgacgattc
     1801 ggcatcggca gctccacctg cacttgatcg attgcggtgg cggtcacttt tactggcagg
     1861 aagttcatct ttggcgaacc gataaatccg gcgacaaaac ggtctgccgg atagtgatac
     1921 agctccagcg gtttcccaac ctgcgccacg cgaccagcgt ccagcaccac gattttgtcg
     1981 gccagcgtca tcgcttcgac ctgatcgtgg gtgacgtaaa tcattgtgcg gcccaggcgt
     2041 ttatgcagac gggagatttc gatacgcatt tgcacacgca gtgcagcatc gaggttggag
     2101 agcggttcat cgagcaaaaa tacgcttggc tcggccacca gcgtacggcc aatcgccaca
     2161 cgctgacgct gaccaccgga gagcgctttc ggtttgcgat ccagcaaatg cgccagttgc
     2221 agcacttccg caacctggtt aactcgctgg ttaatcactt cttttttcgc accagccagt
     2281 ttcaggccaa atgacatgtt ttctgctact gacaggtggg gatagagcgc gtaagactga
     2341 aacaccatac caacgccgcg ttctgcaggc ggagtgtcat tcatccgctt ctcaccgatg
     2401 aacaggtcgc cgctggtgat cgtctcaagc ccggcaatca tgcgcagtaa agtcgattta
     2461 ccgcagccag acggtccgac aaacaccacg aattcacctt catggatatc gagattgata
     2521 tctttcgata ccacgacctc gccccaggct ttcgttacat tttgcagctg tacgctcgcc
     2581 atgcccttct ccctttgtaa caacctgtca tcgacagcaa cattcatgat gggctgacta
     2641 tgcgtcatca ggagatggct taaatcctcc accccctggc ttttttatgg gggaggaggc
     2701 gggaggatga gaacgcagct tctgtgaact aaaccgaggt catgtaagga atttcgtgat
     2761 gttgcttgca aaaatcatgg cgattttatg tgcgcatctc cacattaccg ccaattctgt
     2821 aacagagatc acacaaagca acgatggggc gtaggggcaa ggaggatgga aagaggttgc
     2881 cgtataaaga aactagagtc cgtttaggtg ttttcacgag cacttcacca acaaggacca
     2941 tagattatga aaataaaaac aggtgcacgc atcctcgcat tatccgcatt aacgacgatg
     3001 atgttttccg cctcggctct cgccaaaatc gaagaaggta aactggtaat ctggattaac
     3061 ggcgataaag gctataacgg tctcgctgaa gtcggtaaga aattcgagaa agataccgga
     3121 attaaagtca ccgttgagca tccggataaa ctggaagaga aattccctca ggttgcggca
     3181 actggcgatg gccctgacat tatcttctgg gcgcacgacc gctttggtgg ctacgctcaa
     3241 tctggcctgt tggctgaaat caccccggac aaagcgttcc aggacaagct gtatccgttt
     3301 acctgggatg ccgtacgtta caacggcaag ctgattgctt acccgatcgc tgtcgaagcg
     3361 ttatcactga tttataacaa agatctgctg ccgaacccgc caaaaacctg ggaagagatc
     3421 ccggcgctgg ataaagaact gaaagcgaaa ggtaagagcg cgctgatgtt caacctgcaa
     3481 gaaccatact tcacctggcc gctaattgcg gctgacgggg gttatgcgtt caagtatgaa
     3541 aatggcaagt acgacattaa agacgtaggc gtggataacg ctggcgcgaa agcgggtcta
     3601 accttcctgg ttgatctgat taaaaacaaa cacatgaatg cagacaccga ttactccatc
     3661 gcagaagctg cctttaataa aggcgaaaca gcgatgacta tcaacggccc gtgggcatgg
     3721 tccaacatcg acaccagtaa agtgaattat ggtgtaacgg tactgccgac cttcaaaggt
     3781 caaccatcta aaccgttcgt tggcgtgctg agcgcaggta ttaacgccgc cagtccgaac
     3841 aaagagctgg cgaaagagtt cctcgaaaac tatctgctga ctgacgaagg tctggaagcg
     3901 gtcaataaag acaaaccgct gggtgcagta gcgctgaagt cctatcagga tgaacttgcg
     3961 aaagatccac gtattgccgc caccatggat aacgctcaga aaggtgaaat tatgccgaac
     4021 atcccgcaga tgtccgcttt ctggtatgcc gtgcgtactg cggtgatcaa cgccgccagc
     4081 ggtcgtcaaa ctgtggatgc tgcgctgaaa gatgcacaga ctcgtatcac caagtaatgc
     4141 tgtgaaatgc cggatgcggc gtaaacgcct tatccggcct acgaaattga aacgtatgta
     4201 ggcctgataa gacgcgtcag cgtcgcatca ggcagttgtt gtcggataag gtatgaaagc
     4261 cttatccgtc ctggaatgag gaagaacccc atggatgtca ttaaaaagaa acattggtgg
     4321 caaagcgacg cgctgaaatg gtcagtgtta ggtctgctcg gcctgctggt gggttacctt
     4381 gttgttttaa tgtacgcaca aggggaatac ctgttcgcca ttaccacgct gatattgagc
     4441 tcagcggggc tgtatatttt cgccaatcgt aaagcctacg cctggcgcta tgtttacccg
     4501 ggaatggctg gaatgggatt attcgtcctc ttccctctgg tctgcaccat cgccattgcc
     4561 ttcaccaact acagcagcac taaccagctg acttttgaac gtgcgcagga agtgttgcta
     4621 gatcgctcct ggcaagcagg caaaatctat aattttggtc tttatccggc gggcgatgag
     4681 tggcaactgg cgctcagcga cggcgaaact ggcaaaaatt acctctccga cgctttcaaa
     4741 tttggcggcg agcaaaaact gcaattgaaa gaaagcgcca cgcagcctga aggcgagcgc
     4801 gcgaatctgc gcgtgattac ccagaatcgt cagacactga gtgacattac cgccattctg
     4861 ccggatggca acaaagtgat gatgagctcc ctgcgccagt tttctggcac gcagccgctc
     4921 tacacactcg acggtgacgg cacgctgacc aataatcaga gtggcgtaaa atatcgtcca
     4981 aacgaccaga ttggttttta ccagtccatc actgccgacg gtaactgggg tgatgaaaag
     5041 ctaagccccg gttacaccgt gaccaccggc tggaaaaact ttacccgcgt ctttaccgac
     5101 gaaggcattc agaaaccgtt cctcgccatt ttcgtctgga ccgtggtgtt ctcgctgatc
     5161 actgtctttt taacggtggc ggtcggcatg gttctggcgt gtctggtgca gtgggaagcg
     5221 ttgcgcggca aagcggtcta tcgcgtcctg cttattctgc cctacgcggt gccatcgttc
     5281 atttcaatct tgattttcaa agggttgttt aaccagagtt ttggtgaaat caacatgatg
     5341 ttgagcgcgc tgtttggcgt gaagcccgcc tggttcagcg atccgaccac cgcccgcacg
     5401 atgctgatta tcgtcaacac ctggctgggt tatccgtaca tgatgatcct ctgcatgggc
     5461 ttgctgaaag cgattccgga cgatttgtat gaagcctcag caatggatgg tgcaggtcca
     5521 ttccagaact tctttaagat tacgctgccg ctgctgatta aaccgctgac gccgctgatg
     5581 atcgccagct ttgcatttaa ctttaacaac ttcgtgctga ttcaactgtt aaccaacggc
     5641 ggcccggatc gtcttggcac gaccacgcca gccggttata ccgacctgct tgttaactac
     5701 acctaccgta tcgcttttga aggcggcggg ggtcaggact tcggtctggc agcagcaatt
     5761 gccacgctga tcttcctgct ggtgggcgcg ctggcgatag tgaacctgaa agccacgcga
     5821 atgaagtttg attaagggag ataacaaaaa tggcaatggt ccaaccgaaa tcgcaaaaag
     5881 cacgtttgtt tattactcac ctgctactgc tactttttat cgcggcaatt atgttcccgc
     5941 tgctgatggt cgtcgccatc tcgctgcgtc aggggaactt cgcgaccggt agtctgatcc
     6001 cggagcaaat ctcctgggat cactggaaac tggcgttagg ttttagcgtg gaacaggctg
     6061 atggtcgcat tacgccaccg ccattcccgg tactgctgtg gctgtggaac tcggtaaagg
     6121 tcgccgggat ttccgcgatt ggcattgtgg cgctctccac cacctgcgcc tacgctttcg
     6181 cccgtatgcg ctttccaggc aaagcgacgc tgctgaaagg aatgctgatt ttccagatgt
     6241 tcccggcagt actttcactg gtcgcgttgt atgcgttgtt tgatcgtctg ggtgagtaca
     6301 ttccattcat tggcctgaat actcacggcg gcgtaatttt cgcgtatctg ggcgggattg
     6361 cgctgcatgt ctggaccatc aaaggttatt tcgaaaccat cgacagttcg ctggaagaag
     6421 cggcggcgct ggatggtgcg acaccgtggc aggccttccg ccttgtcctg ttgccgctgt
     6481 cagtaccgat tctggcggtt gtgttcatcc tgtcgtttat cgctgccatt actgaagttc
     6541 cggtcgcgtc gctattactg cgtgacgtaa acagctacac cctggccgtg gggatgcagc
     6601 aatacctcaa cccgcaaaac tacctgtggg gtgactttgc cgccgctgcc gtgatgtctg
     6661 cattaccgat caccatcgtc ttcttgctgg ctcaacgctg gctggtcaac ggcctgacgg
     6721 caggtggtgt gaaaggttaa agatgttgtt ctgccaatgt tatgccgctg caccctcaac
     6781 ttacgttatc ccaacttgtg actgttattc ggcgctccac ggagcgcctt tttttactcc
     6841 cgcttcagcc gtttcgaatt acacagccat agggtgatca ccagtaacag gatcgctgcc
     6901 gagtagatca acacatccag tggcgattta tgatcgacga tgatcaagcg cacaatggcg
     6961 gtgatcccaa tatagacaaa gtaacgtagc gggaagtgaa aaccggactg aaagtacttc
     7021 acaatcagcg cgataaattc gaaatagaga aagtaaacca ccagcccttc taccagctca
     7081 tatttactgg tttgttctgg cgcgaacagc acatcagcca gatgcaccgt ttctttgccg
     7141 aggaagacaa ccaaaatcag gccgaggcac aacaggccaa gattgagtac ggtctgcaaa
     7201 atggtggaga taaactccac gcgcggacga gagagtgacg tcatagcagt acctccttca
     7261 cagtggcgag cttcctgtat aacgcaaaaa tgtgacggag atctatattt tttgtgtatg
     7321 ttttgttcag ctcaattaag tgggcatctc ccatcaacgt cgcatctggc aaaacacaca
     7381 gcccaaaaaa atcacccatt caggtgattc ccagccacca gcaatgcgcg aaaaatcgac
     7441 aatgttcctc tggaacaaat gaaacgtaac ctgataaagg aggtaaaaat gaagccttat
     7501 tttgctgctt tgatgttatc agtctctgtt cttcctgctt atgctggtcc gctcggtact
     7561 gctgataaag ccgacctacc gcaaagcaat gtctccagcc caatgatggc ccagtcgctc
     7621 aggcaaccag acctgcaacc gatatcgacg gacaggaaga cagaatgttt tcgcctttac
     7681 acaccagatc gcaaaccggg ggtgaactgc gttccggatg gctcgacagg ccattaagaa
     7741 tgaaggtgcc agtgagtgcg aaaactggca cctttcccat gacttagcgg aaattgacgc
     7801 tacggtcgct ggtcatatca tacaactgga acttacgccc aagttgctga ccaccgtcac
     7861 gcgtcagcgg cgtccagcca atcgccgcac ggctgcgtgt cgggccagac gagaagagat
     7921 ccagcggtac agagacatac acgcctttgg tgaagtcccc ttcgccgtac tcctctttcg
     7981 aaacattcgt gatcgtggca tagcccccca ctaccacgcc gctatcaaag cgtttggcga
     8041 tctccagcgt gccgccttta tccccagcca gatactgccc gacgctcgct ttcactaaca
     8101 cgtcctgggc gaaagatggc gtccagtacg cggtcagatg tccggttttc acgctgtaat
     8161 cggtgaattt catcatatct tttgcgctac gccagtcgcg ctgtttaacg tagttggcat
     8221 ccagaccaaa cgcccagttg ctatccagcg ggcgatacaa cacttctgcc cccacgccgc
     8281 caaacatggt ttcgagataa cctccgtaga cctgaccgta gaagccgttg cccagatgct
     8341 ggaagtagtt ggcttgcagg ttattcacat agacatcatt ctgcacatac tcgcgcacat
     8401 gggtacgcac gcgcggtaag tgcgagtcct gcggtggatt ggtgtagtta aacttgtcgt
     8461 agttgttggc aaggttagca aacaggctgc cggtggtcag cagatggtcc gtcagccaca
     8521 aatccgccgt tcccatcacg cccagctgat acatgtaaaa gttttccggg ccaccgaccg
     8581 actggttcag caccggatcg atatggaaat caaagcgtga tttgtcgata taccagccct
     8641 gctcggtgga cttcggaacc actggctcga cgcgtttttg caccagcttc gtttcgtggc
     8701 ccaacggctc tccggcgaga tgatttttca ggctggcgac atcggtttcc gtcgtcacct
     8761 gcggcatgtt aaggcgattt tccgtaatgc ggatcgtttt gatcccatcc ggtagatcgt
     8821 tcatcacgat ccgattagca cggatgatcc cttcgcgcgg atcacgatat ttcacctgct
     8881 cgccggtgac atacagcgta tcgcctttcg tctggatctg cggatcggcc agtccggcgt
     8941 tgtatttcag caacgttaac tgattcgcca ccaccgagtg ctgcaaaatg gcatcctgtg
     9001 gctgcggttg atattgcggg cgggcgttat cgttgtagga cgggcgcaga tcgttaaagt
     9061 tggtgcgcag cgtgacgcca aacataaagg tgttgccacg ttcatagcta aggttaacgt
     9121 cggcccaatc ggtaacgcga taaatcgccc cgatgttaaa cttacttttt tgctccagct
     9181 tcccggcaaa atcctgctga taattattac cttcatactc cagtttcagg cgcagcggtt
     9241 gccagggcgt ctggtattcc acgccgccaa acagtgaggc cgggccgtgg aacatctgac
     9301 tgccgtcgat agatcccgct tgtttgtagc tgttatcgcg atagcaatat ttatcactgg
     9361 ctgaacagag cggatttttc acattaccgc tggtgcccag atagccccat cccaggccga
     9421 gcgtaaaatc gaacggcccc caggctttgc tggcaacaag atattccgca tcaaacagcc
     9481 ccgtaccgcc gatatcccgc gcaccaaccg ccacttgcgg cagccaataa ctctcttccc
     9541 acaaacgcag tttgagatcg aaggctttat ctttatacgt ttgatcgcca gagaacgctt
     9601 cgacgctgct gtactgccgg gtgcgcacgt cggtgtagcg cagcgttgtt tccagccacg
     9661 ggaagagttg cactgaagct gagtaataac ggtactgatc gttatcgcga tagttcaggc
     9721 tcagctcccc ttcccgcgcc atgcgcgcgg tgggcgtttg taataatcct acgccaccga
     9781 aatccgattg cgacggacca atgggcgcag gatatgtttc gccgtagcaa gctgtgctaa
     9841 tgcccagcgc cagtaagcta agcagatgtc tttttttcat tattggggta tccgctgcgt
     9901 cagagtctga agaatgtcgg cgttaagggc atcaggtgtc tcactccaga cggagtccgc
     9961 gaggccaaca taaataatgc tgccgggcat cggctctacg tgacgcttgt tccaataagc
    10021 caccggcgct ttttgcgtgc gtccgtccgg gtaaaccacc cacgcgtagc tgcgatccgc
    10081 accgctgagc aggctctggt cagagagata gctcgccacg tcgcgacctg gagtgaatga
    10141 ctgcttgcca ggacgactga taagcccgaa caacgtgacc gtggacggcg gcgttccgac
    10201 ccacagcgtg tagttgccct gcaacggcgg gttaccgcgt tcggcaacgc ggacgatatc
    10261 aggatcaaga ttgatttttt gcctgcccgt caccttcaac gcctgaattt gctggcgtaa
    10321 ggcgttaatc gcggcggcat cgtcggcgct ggaatctgca gccagttctg ccagccgtgt
    10381 cagcaacgcc tgctgctgac gcaatgcagc cgccgtggcc agctcttcgc taatcaccgc
    10441 tccgggccac cagctgtttg ccagccgcgg ttggcctacc agatcgatta aatgctctgc
    10501 gcccgtcagc gttttggcct cactgctgcc attgctgaac accttgacgg ttcccgccgc
    10561 gaagactgat gacgcccccg cactcaaaag caacgcgaca atagtttgtt taatcattgt
    10621 tttgccgcct tgatgagggt ggttttcacc gggaagtaat ccgcgccgag atactgttcc
    10681 gactggcgaa tttgcccttc gctgtcgatc cagtagcggt tatgccagcg cgcctggtcg
    10741 gtggagactt cttcgtccag cacgcgaacc ggggtttcat cgctgccgac tttgacggta
    10801 tcgatgccat cccatttgaa gactgagcgt gcggtggcgt agcgtacctg ctggtactcg
    10861 gtccagccca tcgtgcgcgt ccaggttgca ccatcaatga tttgtgcggg tttaatcagc
    10921 gggtcagtgg caaggttatt cacttcaatc aggttgtcgc caccaagcaa agtcttcacc
    10981 agacgaccat gttgtgtgac aagggtggcc tgatcctgag tgacccattt ttgttgtccg
    11041 tcttcagcga aggccagcac cacaaataac tgcggcccgc cattaagctg catgtactgg
    11101 ctggcgtagg gcatattttg aatatcatcg tccgtcagct gtacgcctgg cgtaccgaac
    11161 agactgtccc acagtgaatt gcccagctct ttagtcgtgg ctgaacaggc ctgtaatagc
    11221 aggcagataa gaatgagtgc tggtcgcttc acgactcttc tccagagggg cagaataacc
    11281 acaccggagt gtggttatgg ttaatcccct tcatacttca agcttcagat gcgatgactc
    11341 gaattatttt ggggatacat actttattac tgggtactgg tggtcgtggt tgtggtggtc
    11401 ccggtattag aaccatcacc gccaccggtt gccgcgagcg cgacacccac cgccgagctt
    11461 acggtgctga cgctggtcgc ggaggaacta cccgccgaaa ccgacgttgc tgccgatcct
    11521 gccgcttcgc ccacctgtac aggtgcagcc cacgcagaag tcgccgcaag cgcagatatg
    11581 gcaaaaatgc catacagaac ttttttcata acaatttccc ttcattgaat gaatggagat
    11641 ttacccaaaa catttcgggc gggatcgagt atacacaact aaagcatgcg aattacggcg
    11701 cggggaatta gtgatggagt tttagg
//
LOCUS       DADSYM010000143        11352 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2415
            SAMN08039579-rid11723173.denovo.144, whole genome shotgun sequence.
ACCESSION   DADSYM010000143 DADSYM010000000
VERSION     DADSYM010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039579
            Sequence Read Archive: SRR6362533
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11352)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11352)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980162.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 03:39:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,484
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 4,347
            CDSs (with protein)               :: 4,347
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 43 of 85
            Pseudo Genes (incomplete)         :: 37 of 85
            Pseudo Genes (internal stop)      :: 19 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11352
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039579-rid11723173.denovo.144"
                     /strain="LGE2415"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8302 N 95.9628 W"
                     /collection_date="2015-08-24"
     gene            63..1118
                     /gene="phoE"
                     /locus_tag="J1Y83_003553"
     CDS             63..1118
                     /gene="phoE"
                     /locus_tag="J1Y83_003553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoporin PhoE"
                     /protein_id="HBA7631668.1"
                     /translation="MKKSTLALVVMGIVASVSVQAAEIYNKDGNKLDVYGKVKAMHYM
                     SDNDSKDGDQSYIRFGFKGETQINDQLTGYGRWEAEFAGNKAESDTAQQKTRLAFAGL
                     KYKDLGSFDYGRNLGALYDVEAWTDMFPEFGGDSSAQTDNFMTKRASGLATYRNTDFF
                     GVIDGLNLTLQYQGKNENRDVKKQNGDGFGTSLTYDFGGSDFAISGAYTNSDRTNEQN
                     LQSRGTGKRAEAWATGLKYDANNIYLATFYSETRKMTPISGGFANKTQNFEAVAQYQF
                     DFGLRPSLGYVLSKGKDIEGIGDEDLVNYVDVGATYYFNKNMSAFVDYKINQLDSDNK
                     LNINNDDIVAVGMTYQF"
     gene            complement(1228..1629)
                     /locus_tag="J1Y83_003554"
     CDS             complement(1228..1629)
                     /locus_tag="J1Y83_003554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-binding protein Crl"
                     /protein_id="HBA7631669.1"
                     /translation="MTLPSGHPKSRLIKKFTALGPYIREGKCEDNRFFFDCLAVCVNV
                     KPAPEVREFWGWWMELEAQESRFTYSYQFGLFDKAGDWTSVQIKDAEVVERLEHTLRE
                     FHEKLRELLATLNLKLEPADDFRDEPVKLTA"
     gene            complement(1687..2931)
                     /gene="frsA"
                     /locus_tag="J1Y83_003555"
     CDS             complement(1687..2931)
                     /gene="frsA"
                     /locus_tag="J1Y83_003555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase FrsA"
                     /protein_id="HBA7631670.1"
                     /translation="MTQANLSETLFKPRFKHPETSTLVRRFSHGAQLPVQSALDGKTI
                     PHWYRMINRLMWIWRGIDPREILDVQARIVMSDAERTDDDLYDTVIGYRGGNWIYEWA
                     TQAMVWQQKACAEEDPQLSGRHWLHAATLYNIAAYPHLKGDDLAEQAQALSNRAYEEA
                     AQRLPGTMRQMEFTVPGGAPITGFLHMPKGDGPFPTVLMCGGLDAMQTDYYSLYERYF
                     APRGIAMLTIDMPSVGFSSKWKLTQDSSLLHQHVLKALPNVPWVDHTRVAAFGFRFGA
                     NVAVRLAYLESPRLKVVACLGPVVHTLLSDFKCQQQVPEMYLDVLASRLGMHDASDEA
                     LRVELNRYSLKVQGLLGRRCPTPMLSGYWKNDPFSPEEDSRLITSSSADGKLLEIPFN
                     PVYRNFDKGLQEITDWIEKRLC"
     gene            complement(3023..3481)
                     /gene="gpt"
                     /locus_tag="J1Y83_003556"
     CDS             complement(3023..3481)
                     /gene="gpt"
                     /locus_tag="J1Y83_003556"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HBA7631671.1"
                     /translation="MSEKYIVTWDMLQIHARKLASRLMPSEQWKGIIAVSRGGLVPGA
                     LLARELGIRHVDTVCISSYDHDNQRELKVLKRAEGDGEGFIVIDDLVDTGGTAVAIRE
                     MYPKAHFVTIFAKPAGRPLVDDYVVDIPQNTWIEQPWDMGVVFVPPISGR"
     gene            3742..5199
                     /gene="pepD"
                     /locus_tag="J1Y83_003557"
     CDS             3742..5199
                     /gene="pepD"
                     /locus_tag="J1Y83_003557"
                     /EC_number="3.4.13.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008783788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytosol nonspecific dipeptidase"
                     /protein_id="HBA7631672.1"
                     /translation="MSELSQLSPQPLWDIFAKICSIPHPSYHEEQLAEYIVGWAKEKG
                     FHVERDQVGNILIRKPATAGMENRKPVVLQAHLDMVPQKNNDTVHDFTKDPIQPYIDG
                     EWVKARGTTLGADNGIGMASALAVLADENVVHGPLEVLLTMTEEAGMDGAFGLQSNWL
                     QADILINTDSEEEGEIYMGCAGGIDFTSNLHLDREAVPAGFETFKLTLKGLKGGHSGG
                     EIHVGLGNANKLLVRFLAGHAEELDLRLIDFNGGTLRNAIPREAFATIAVAADKVDAL
                     KSLVDTYQEILKNELVEKEKNLALLLDSVANDKAALIAKSRDTFIRLLNATPNGVIRN
                     SDVAKGVVETSLNVGVVTMTDNNVEIHCLIRSLIDSGKDYVVSMLDSLGKLAGAKTEA
                     KGAYPGWQPDANSPVMHLVRETYQRLFNKTPNIQIIHAGLECGLFKKPYPEMDMVSIG
                     PTITGPHSPDEQVHIESVGHYWTLLTELLKEIPAK"
     gene            complement(5256..5870)
                     /locus_tag="J1Y83_003558"
     CDS             complement(5256..5870)
                     /locus_tag="J1Y83_003558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000602105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor H"
                     /protein_id="HBA7631673.1"
                     /translation="MILLQLSSAQGPEECCLAVKKALDRLIKEATRQDVAVTVLETET
                     GRYSDTLRSALISLDGDNAWALSESWCGTIQWICQSPYRPHHGRKNWFLGIGRFTADE
                     QEQSDAIRYETLRSSGPGGQHVNKTDSAVRATHLASGISVKVQSERSQHANKRLARLL
                     IAWKLEQQQQENSAALKSQRRMFHHQIERGNPRRTFTGMAFIEG"
     gene            complement(5867..7018)
                     /locus_tag="J1Y83_003559"
     CDS             complement(5867..7018)
                     /locus_tag="J1Y83_003559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015912462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA ligase RtcB family protein"
                     /protein_id="HBA7631674.1"
                     /translation="MEWYMGKYIRPLSDAVFTIASDDLWIESLAIQQLHTTANLPNMQ
                     RVVGMPDLHPGRGYPIGAAFFSVGRFYPALVGNDIGCGMALWQTDILARKYNADKFEK
                     RLSDLDDVAEESWLEENLPSAFAQHPWRNSLGSIGGGNHFAELQQIDQIIDAELFALA
                     GLDAQHLQLLVHSGSRGLGQSILQRHIASFSHHGLPEGSDDALRYIAEHDDALAFARI
                     NRQLIALRIMQQVKATGSPVLDVAHNFVSACQIGDQQGWLHRKGATPDDNGLVIIPGS
                     RGDYSWLVKPVANEKTLHSLAHGAGRKWGRTECKGRLAAKYTATQLSRTELGSRVICR
                     DKQLIFEEAPQAYKSAESVVQCLVLAGLIIPVARLRPVLTLKNSGGKKG"
     gene            complement(7196..7648)
                     /locus_tag="J1Y83_003560"
     CDS             complement(7196..7648)
                     /locus_tag="J1Y83_003560"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBA7631675.1"
                     /translation="MNNIQIRNYQPGDFQQLCAIFLRAVTMTASQHYSPQQIAAWAQI
                     DESRWKEKLAKSQVRVAVINAQPVGFITRIGHYIDMLFVEPEYTRRGVASALLKPLIK
                     SESELTVDASITAKPFFERYGFQTVKQQRVECRGTWFTNFYMRYKPQH"
     gene            complement(7645..8700)
                     /gene="dinB"
                     /locus_tag="J1Y83_003561"
     CDS             complement(7645..8700)
                     /gene="dinB"
                     /locus_tag="J1Y83_003561"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HBA7631676.1"
                     /translation="MRKIIHVDMDCFFAAVEMRDNPALRDIPIAIGGSRERRGVISTA
                     NYPARKFGVRSAMPTGMALKLCPHLTLLPGRFDAYKEASNHIREIFSRYTSRIEPLSL
                     DEAYLDVTDSVHCHGSATLIAQEIRQTIFNELQLTASAGVAPVKFLAKIASDMNKPNG
                     QFVITPAEVPAFLQTLPLAKIPGVGKVSAAKLEAMGLRTCGDVQKCDLVTLLKRFGKF
                     GRILWERSQGIDERDVNSERLRKSVGVERTMAEDIHHWSECEAIIERLYPELERRLAK
                     VKPDLLIARQGVKLKFDDFQQTTQEHVWPRLNKSDLIATARKTWDERRGGRGVRLVGL
                     HVTLLDPQMERQLVLGL"
     gene            complement(8771..9559)
                     /gene="lafU"
                     /locus_tag="J1Y83_003562"
     CDS             complement(8771..9559)
                     /gene="lafU"
                     /locus_tag="J1Y83_003562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011076207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative lateral flagellar export/assembly
                     protein LafU"
                     /protein_id="HBA7631677.1"
                     /translation="MVTTIKLIVNSVSKSERESIIAALHGQSIFNGGGLSPLNKISPS
                     HLPKPATVAVPEETEKKARDVNEKTALLKKKSATELGELATSINTIARDAHMEANLEM
                     EIVPQGLRVLIKDDQNRNMFERGSAKIMPFFKTLLVELAPVFDSLDNKIIITGHTDAM
                     AYKNNIYNNWNLSGDRALSARRVLEEAGMPENKVMQVSAMADQMLLDAKNPQSAGNRR
                     IEIMVLTKSASDTLYQYFGQHGDKVVQPLVQKLDKQQVLSQRAR"
     gene            9501..11240
                     /gene="flhA"
                     /locus_tag="J1Y83_003563"
     CDS             9501..11240
                     /gene="flhA"
                     /locus_tag="J1Y83_003563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system protein
                     FlhA"
                     /protein_id="HBA7631678.1"
                     /translation="MLSRSDLLTLLTINFIVVTKGAERISEVSARFTLDAMPGKQMAI
                     DADLNAGLINQAQAQTRRKDVASEADFYGAMDGASKFVRGDAIAGMMILAINLIGGVC
                     IGIFKYNLSADAAFQQYVLMTIGDGLVAQIPSLLLSTAAAIIVTRVSDNGDIAHDVRN
                     QLLASPSVLYTATGIMFVLAVVPGMPHFPFLMFSALLGFTGWRMSKRPQAAEAEEKSL
                     ETLTRTMTETSEQQVSWETIPLIEPISLSLGYKLVALVDKAQGNPLTQRIRGVRQVIS
                     DGNGVLLPEIRIRENFRLKPSQYAIFINGIKADEADIPADKLMALPSSETYGEIDGVL
                     GNDPAYGMPVTWIQPAQKAKALNMGYQVIDSASVIATHVNKIVRSYIPDLFNYDDITQ
                     LHNRLSSMAPRLAEDLSAALNYSQLLKVYRALLTEGVSLRDIVTIATVLVASSAVTKD
                     HILLAADVRLALRRSITHPFVRKQELTVYTLNNELENLLTNVVNQAQQAGKVMLDSVP
                     VDPNMLNQFQSTMPQVKEQMKAAGKDPVLLVPPQLRPLLARYARLFAPGLHVLSYNEV
                     PDELELKIMGALM"
BASE COUNT         2759 a         2962 c         2896 g         2735 t
ORIGIN      
        1 tcctgttttc aacggaactc tcccgctgaa tattcgcgcg ttaattaaaa tcaggaatga
       61 aaatgaaaaa gagcactctg gcattagtgg tgatgggcat tgtggcatct gtatccgtac
      121 aggccgcaga aatatataac aaagacggta ataaactgga tgtctatggc aaagttaaag
      181 ccatgcatta tatgagtgat aatgacagta aagatggcga ccagagttat atccgttttg
      241 gttttaaagg cgaaacacaa attaacgatc aactgactgg ttatggtcgt tgggaagctg
      301 agtttgccgg taataaagcg gagagtgata ctgcacagca aaaaacgcgt ctcgcttttg
      361 ccgggttgaa atataaagat ttgggttctt tcgattatgg tcgtaacctg ggcgcgttgt
      421 atgacgtgga agcctggacc gatatgttcc cggaatttgg tggcgactcc tcggcgcaga
      481 ccgacaactt tatgaccaaa cgcgccagcg gtctggcgac gtatcggaac accgacttct
      541 tcggcgttat cgatggcctg aacttaaccc tgcaatatca agggaaaaac gaaaaccgcg
      601 acgttaaaaa gcaaaacggc gatggcttcg gcacgtcatt gacatatgac tttggtggca
      661 gcgatttcgc catcagtgga gcctatacca actctgatcg caccaacgag cagaacctgc
      721 aaagccgtgg cacgggcaag cgtgcagaag catgggcaac aggtctgaaa tacgatgcca
      781 ataatattta tctggcaact ttttattctg aaacgcgtaa aatgacgcca atctctggcg
      841 gttttgccaa taagacgcag aactttgaag cggtcgctca ataccagttt gactttggtc
      901 tgcgtccatc gctgggttat gtcttatcga aagggaaaga tattgagggc atcggtgatg
      961 aagatctggt caattatgtc gatgttggtg ctacgtatta tttcaacaaa aatatgtcag
     1021 cgtttgttga ttataaaatc aaccaactgg atagcgataa caaattgaat attaataatg
     1081 atgatattgt cgcggttggg atgacgtatc agttttaatg aatattgccg gatgcggcgt
     1141 gaacgcctta tccggcctac gaaaactacg aaaaccaata tttcaggccg gaagagatgc
     1201 tctaacatcg catccggcac atttcattca cgccgttaac ttcaccggtt catcacgaaa
     1261 atcatctgcc ggttccagtt tcagattcag cgtcgccagc agctcacgca gcttctcgtg
     1321 aaattcacgc agggtgtgct ccagtcgttc aaccacttcg gcatctttta tctgaacact
     1381 cgtccagtcg cctgctttat cgaacagacc aaactggtaa ctgtaggtaa aacgggattc
     1441 ctgcgcttcc agttccatcc accagcccca gaactcacgc acttccggtg ccggtttcac
     1501 gttgacgcat acagccagac aatcgaaaaa gaatcgatta tcttcgcact taccttcacg
     1561 aatatacggg cctagtgcgg taaatttttt gatcaatctg ctcttcgggt gtccactcgg
     1621 taacgtcatt gcaatctcct gttgtgatgc aactgtttta ccaaattggt aaaatttagc
     1681 aaatttttaa cacaaacgtt tttcgatcca gtctgtgatt tcctgaaggc ctttgtcaaa
     1741 attccgatac actgggttaa atgggatctc taataattta ccgtcagcag atgatgaggt
     1801 gattaagcgt gagtcctctt ccgggctgaa cggatcgttc ttccagtagc ctgataacat
     1861 tggcgttggg cagcgacgtc ccagcaatcc ttgcactttt aatgaatagc gattcaactc
     1921 cactcgcaac gcttcgtcgg aagcatcatg catccccaaa cgactggcca gaacgtcaag
     1981 atacatttcc ggcacctgtt gttggcactt aaaatcactc aacagggtat gaactaccgg
     2041 accaagacag gcaaccactt tcagacgcgg cgattcaagg tatgccagac gcacggcaac
     2101 gttagcgccg aaacggaaac caaaggccgc gacgcgagtg tgatccaccc acggtacgtt
     2161 aggcagcgcc tttaaaacgt gctgatgcaa caggctggag tcctgggtga gcttccattt
     2221 tgaagaaaag cccaccgacg gcatatcaat agtcagcata gcaatgccgc gcggcgcaaa
     2281 ataacgttca tacaggctgt aatagtccgt ctgcatcgca tccagaccac cacacattaa
     2341 tactgtcggg aacgggccat cgcctttcgg catatgcaaa aagccggtga tgggcgcacc
     2401 gccgggtacg gtaaactcca tctgccgcat cgtgcccggc agacgctgag cggcctcttc
     2461 ataggcgcgg tttgacaaag cctgcgcttg ctcggccagg tcatctcctt tcagatgagg
     2521 ataggcagca atgttgtaca acgtagccgc atgcagccag tgtcgaccgc tgagttgcgg
     2581 atcttcttcc gcacaggctt tttgttgcca caccatcgcc tgggtagccc actcataaat
     2641 ccagttgccg ccgcggtagc caatcaccgt atcgtataaa tcatcgtcgg tacgttcggc
     2701 atcgctcatc acaatacgcg cctggacatc gaggatttca cgtgggtcaa tgccgcgcca
     2761 gatccacatc agacggttaa tcatgcgata ccagtgaggg atggttttac catcaagggc
     2821 cgactgcaca ggcagttgtg cgccgtgact aaagcggcgg actagcgtcg aggtttcagg
     2881 atgtttaaag cggggtttga acagggtttc gctcaggttt gcctgtgtca tggatgcagc
     2941 ctccagaata cttactggaa actattgtaa cccgcctgaa gttaaaaaga acaacgcccg
     3001 gcagtgccag gcgttgaaaa gattagcgac cggagattgg cgggacgaat acgacgccca
     3061 tatcccacgg ctgttcgatc caggtatttt gcggaatatc aacaacataa tcatcaacca
     3121 gcggacgacc ggccggtttt gcgaagatgg tgacaaagtg cgcttttgga tacatttcac
     3181 gaatcgcaac cgcagtacca ccggtatcta ccaggtcatc aataacgatg aagccttcgc
     3241 catcgccttc tgcgcgtttc agcactttaa gctcgcgctg gttgtcgtga tcgtagctgg
     3301 aaatacaaac ggtatcgaca tgacgaatac ccagttcacg cgccagtaac gcacccggta
     3361 ccagaccgcc acggcttacg gcaataatgc ctttccattg ttcagaaggc atcagtcggc
     3421 ttgcgagttt acgtgcatgg atctgcaaca tgtcccaggt gacgatgtat ttttcgctca
     3481 tgtgaagtgt cccagcctgt ttatctacgg cttaaaaagt gttcgagggg aaaataggtt
     3541 gcgcgagatt atagagatct gacgcactaa aaaccagtat ttcacatgag tccgcgtctt
     3601 tttacgcact gcctctccct gacgcgggat aaagtggtat tctcaaacat atctcgcaag
     3661 cctgtcttgt gttgacaaca ttttctgcta accctgtgac ctgcaatact gttttgcggg
     3721 tgatcgacaa ggagacttaa cgtgtctgaa ctgtctcaat tatctccaca gccgctgtgg
     3781 gatatttttg ccaaaatctg ttctattcct cacccgtcct atcatgaaga gcaactcgct
     3841 gaatacattg ttggttgggc aaaagagaaa ggtttccatg tcgaacgcga tcaggtaggt
     3901 aatatcctga ttcgtaaacc tgctaccgca ggtatggaaa atcgtaaacc ggtcgtcttg
     3961 caggcccacc tcgatatggt gccgcaaaaa aacaacgaca ccgtgcatga cttcactaaa
     4021 gatcctatcc agccttatat tgatggcgaa tgggttaaag cacgcggcac cacgctgggt
     4081 gcggataacg gcattggtat ggcctctgca ctggcggttc tggctgacga aaacgtggtt
     4141 cacggcccgc tggaagtgct gctgaccatg accgaagaag ccggtatgga cggtgctttt
     4201 ggcttacaga gcaactggtt gcaggctgat attctgatta acaccgactc cgaagaagaa
     4261 ggtgaaatct acatgggttg tgcgggaggt atcgacttca cctccaacct gcatttagat
     4321 cgtgaagcgg ttccagctgg ttttgaaacc ttcaagttaa ccttaaaagg tctgaaaggc
     4381 ggtcactccg gcggtgaaat ccacgttggc ctgggtaatg ccaacaaact gttggtgcgc
     4441 ttcctggcgg gtcatgcaga agaactggac ctgcgtctta ttgatttcaa cggcggcaca
     4501 ctgcgtaacg ccatcccgcg tgaagccttt gcgaccattg ctgtcgcagc tgataaagtc
     4561 gacgccctga aatctctggt ggatacctat caggagatcc tgaaaaacga actggtagag
     4621 aaagagaaaa atctggcatt gctgctggac tctgttgcga acgataaagc tgccctgatt
     4681 gcgaaatctc gcgatacctt tattcgtctg ctgaacgcca ccccgaacgg tgtgatccgc
     4741 aactccgacg tggcaaaagg tgtggtcgaa acctccctga acgtcggtgt ggtgaccatg
     4801 accgacaata acgtagaaat tcactgcctg atccgttctc tgatcgacag cggtaaagac
     4861 tacgtagtga gcatgctgga ttcgctgggt aaactggcag gtgcgaaaac cgaagcaaaa
     4921 ggcgcatatc ctggctggca gccggacgct aactctccgg taatgcatct ggtacgtgaa
     4981 acctatcagc gtctgttcaa caaaacgccg aacatccaga ttatccacgc gggcctcgaa
     5041 tgtggtctgt tcaaaaaacc gtatccggaa atggacatgg tgtctatcgg gccaaccatc
     5101 accggtccac actccccgga tgagcaagtt catatcgaaa gcgttggtca ttactggacg
     5161 cttctgactg aactgctgaa agaaattccg gcgaagtaat tatttgattt gctgccggat
     5221 ggcgtttaat cgccttccgg cagtttcatc cttcattatc cttcgataaa agccatccct
     5281 gtaaacgttc gtcgcgggtt gccacgttca atctggtgat ggaacattcg ccgctgcgat
     5341 ttcagcgccg cgctattttc ctgttgctgc tgctccagct tccaggcgat aagcaatcgc
     5401 gccagccgtt tgttggcatg ctgactacgc tctgactgaa ccttcacgct aattccggat
     5461 gccagatgcg tggcgcgtac cgccgagtcg gttttattga catgttgacc gcccggcccc
     5521 gacgaacgca gcgtctcata ccggatcgca tcggattgct cctgctcatc agcggtaaaa
     5581 cgcccaatgc ccagaaacca gtttttgcgc ccatgatgtg gccgatacgg actctgacaa
     5641 atccactgaa tagtgccgca ccagctttcg cttaaagccc atgcgttatc gccatccaga
     5701 gaaatcagcg ccgaacgcag tgtgtcagag tagcgacccg tttctgtttc cagcaccgtt
     5761 accgcgacgt cctgtcgggt agcttcttta atcagcctgt ccagtgcttt tttcactgcg
     5821 agacaacatt cttccggccc ctgagcagag gagagttgta gcaagatcat ccttttttcc
     5881 ctccactgtt tttgagcgtc agcaccgggc gcagtcgcgc gacaggaata attaacccag
     5941 ccagcaccag acattgcacc acgctttcag ccgatttata ggcttgtggc gcttcttcaa
     6001 agatgagttg tttatcgcga caaattaccc gactgccaag ttcagtccgc gagagttgcg
     6061 tcgccgtgta tttcgctgcc agacgccctt tacactcggt gcgcccccat ttacgccccg
     6121 ccccatgcgc cagcgagtgg agcgtttttt cgttcgcaac aggtttaacc agccaggagt
     6181 aatcgccgcg tgaaccggga ataatcacca gaccgttgtc atccggcgtg gcgcctttac
     6241 gatgcaacca gccctgttga tcgccgattt ggcacgcgct aacaaagtta tgcgccacat
     6301 ccagaaccgg acttccggtg gccttaactt gttgcattat gcgcaaagcg atcagctggc
     6361 gattaatacg cgcaaacgcc agcgcatcgt catgttccgc aatataccgt agcgcgtcgt
     6421 cactgccttc aggcaaacca tgatgcgaaa acgaggcaat atgccgctgt aaaatagact
     6481 gccccaatcc cctcgagccg ctatgaacca gcagttgcag atgctgcgca tccagaccag
     6541 ccagtgcaaa cagttccgcg tcgataattt gatcaatttg ttgcagttct gcgaagtggt
     6601 taccgccacc gatggagcca agcgagttgc gccacggatg ctgtgcaaat gctgacggca
     6661 ggttttcctc cagccagctt tcttcagcaa cgtcatccag atctgataat cgcttttcaa
     6721 acttatcggc gttgtatttg cgagcgagaa tatctgtttg ccatagcgcc atgccgcagc
     6781 cgatatcatt gccgaccagt gccgggtaaa aacgaccaac agagaagaac gctgcgccaa
     6841 tcgggtagcc gcgtccggga tgtaaatctg gcatccccac aacgcgctgc atgttgggta
     6901 aatttgccgt ggtgtgtaat tgttggatcg ctaaactctc gatccacagg tcatcagatg
     6961 cgatggtaaa taccgcatcg gataagggac gaatatattt gcccatgtac cattccattt
     7021 atgaataaaa atcaggaaat ggcagacgaa tccagggcaa gaaaaaagaa ttaatcctgg
     7081 aaaggtctgc aaagagtcat tgataaagga tttgtcatga tttacctcct tgcagtttgt
     7141 ggaataagaa aacgcgcaca tactagacgg gcgttatttt cattgcaagc tggatttaat
     7201 gttgcggttt atatcgcata taaaaattag taaaccacgt tccccggcat tcaacgcgct
     7261 gctgcttaac tgtctgaaaa ccataacgtt caaaaaaggg ttttgcggtt atgctggcgt
     7321 ccaccgtaag ttcggattca gacttaatca atggttttaa caaagcgctg gcaactccgc
     7381 gtcgggtgta ttccggttca acaaataaca tatcgatata atgtccaatg cgggtaataa
     7441 aaccaaccgg ttgtgcatta ataactgcaa cccgcacttg tgatttcgcg agtttctcct
     7501 tccagcgaga ttcgtcaatc tgcgcccagg cggcaatttg ctgtggagaa tagtgctgac
     7561 tcgccgtcat cgtaacagct cgaaggaaaa tagcgcatag ttgctgaaaa tcaccaggct
     7621 gatagtttct tatttgtatg ttattcataa tcccagcacc agttgtcttt ccatttgcgg
     7681 gtccagcaac gtcacatgca gccccaccag tcgcacaccg cgcccgccgc ggcgttcatc
     7741 ccaggtttta cgcgcggtgg cgattagatc agatttattc agccgcggcc agacgtgttc
     7801 ctgggtggtt tgctgaaaat cgtcgaactt taatttcacc ccctggcggg caatcagcaa
     7861 atcaggtttt acctttgcca aacgacgctc cagttcaggg taaagccgct cgatgatcgc
     7921 ttcacattca gaccagtgat gaatatcttc cgccatcgta cgttctacac cgacggattt
     7981 tcgcaacctt tcgctgttaa cgtcgcgctc gtcaatgccc tgactacgct cccacaaaat
     8041 gcggccaaat ttgccaaaac gtttgagcag agtcaccaga tcacactttt gcacatcacc
     8101 gcaggttcgt agccccatcg cttccagttt agccgctgag actttgccga cgccgggaat
     8161 ttttgccagc ggtaaggttt gcagaaatgc cggaacctca gccggagtaa tcacaaactg
     8221 accgttgggt ttattcatgt cggaggcgat tttggcgaga aacttgaccg gcgcaacgcc
     8281 cgcagacgcc gtcagttgca gctcgttgaa gatcgtctgg cggatttcct gggcgatgag
     8341 ggtcgcagaa ccgtggcaat ggacgctatc ggtgacatcg agataggctt catccagtga
     8401 taacggttca atgcgcgagg tatagcgcga gaaaatttcg cggatatgat ttgaggcttc
     8461 tttgtaggcg tcaaagcgcc ctggaagcaa ggtgagatgc gggcataatt tgagcgccat
     8521 ccctgtcggc atagcgctac gtacgccaaa tttacgcgcg ggataattgg cggtgctgat
     8581 taccccccgt cgttcgcggc tgccgccaat agcaataggg atatcgcgca gggcgggatt
     8641 gtcgcgcatc tccaccgctg cgaaaaagca gtccatatcc acatgaatga ttttacgcat
     8701 tgctcacctc tcaacactgg taaagtatac agtgatttca gggtttgaga aatgcgtaaa
     8761 gattcagcat ttaacgcgct cgctgcgaaa gcacctgctg cttatccagc ttttgcacca
     8821 gcggctgcac cactttatcc ccatgctgac caaagtattg atacagcgta tcggacgcac
     8881 ttttggtcag caccataatc tcaatgcgcc ggttgcccgc gctttgcgga tttttggcat
     8941 ccagcagcat ctggtccgcc attgcgctta cctgcatcac tttattttcc ggcattccgg
     9001 cctcttccag cacccgacga gccgaaagcg cgcgatcgcc cgaaaggttc cagttgttgt
     9061 agatattgtt tttgtaggcc atcgcatcgg tatgcccggt aataataatt ttgttatcga
     9121 gcgagtcgaa cactggcgca agctccacca gcaaagtttt aaagaacggc ataatcttcg
     9181 cgctgccgcg ttcaaacata ttgcggttct ggtcgtcttt aatcagcacg cgtaatccct
     9241 ggggaacaat ctccatctcc agattcgctt ccatatgcgc atcacgggcg atggtattga
     9301 tactggtcgc cagctcgcca agctcggtgg cggatttctt cttcaacagc gccgttttct
     9361 cgttgacgtc gcgcgctttt ttctccgtct cttccggcac cgccacggtg gcaggttttg
     9421 gcagatgaga cgggctgatt ttatttaacg gcgacaaccc accaccgtta aaaatcgact
     9481 gcccgtgcag cgcggcgata atgctttcac gctcggattt actgacgctg ttgacaatca
     9541 actttatcgt cgtaaccaag ggggccgagc gtatttccga ggtttctgcc cgctttaccc
     9601 tggacgcgat gcccggcaaa cagatggcga ttgacgccga ccttaacgcc gggttgatca
     9661 atcaggcgca ggcgcagacc cggcgtaaag atgtcgccag cgaggccgat ttctacggcg
     9721 caatggacgg ggcatcaaaa tttgtgcgcg gggacgccat cgccgggatg atgattctgg
     9781 cgatcaacct gatcggcggc gtctgtatcg gcatcttcaa atacaacctg agcgccgacg
     9841 ccgccttcca gcaatatgtg ctgatgacta tcggcgatgg gctggtggcg caaatccctt
     9901 ccctgctgct ctccaccgcg gcggcgatta tcgtcacccg cgtcagcgat aacggtgata
     9961 tcgcccatga cgtgcgtaat cagttgctgg caagcccgtc ggttctctac accgccactg
    10021 gaattatgtt cgtgctggcg gtggtgccgg gaatgccgca tttcccgttc ctgatgttca
    10081 gcgccctgct tggttttacc ggctggcgga tgagcaagcg cccgcaagcg gcggaggcgg
    10141 aagagaaaag cctcgaaacg ctaacccgta cgatgacaga aaccagcgag caacaggtca
    10201 gttgggaaac tattccgctg atcgaaccta tcagtttaag cctcggctac aaactggtgg
    10261 cgctggtgga taaagcgcag ggcaacccgc tcacccagcg tattcgcggc gtacgtcagg
    10321 tgatttccga cggcaacggc gtgctgctgc cggagatccg tattcgggaa aacttccgcc
    10381 tcaagcccag ccagtacgcc attttcatca acggcattaa ggctgatgaa gcggatattc
    10441 cggcagataa actgatggcc ctgccctcca gcgaaaccta cggcgagata gacggcgtgc
    10501 tggggaacga cccggcgtac gggatgccgg tcacctggat tcagcccgcg cagaaagcga
    10561 aggcgctgaa tatggggtat caggtgatcg acagcgccag cgtaattgcc acccatgtga
    10621 ataagattgt gcgcagctat attcctgatt tgtttaacta tgacgatatt actcagctgc
    10681 ataaccgttt gtcgtcgatg gcaccgcgac tggcggaaga tttgagcgcg gcgctcaatt
    10741 acagccagtt gctgaaagtg taccgggcgc tactgactga aggcgtttcc ctgcgcgata
    10801 tcgtcaccat cgccaccgtg ttggtcgcca gtagtgcggt gactaaagat catattctgc
    10861 tggcggccga tgtgcgcctg gcgctgcggc gcagcattac ccatccgttc gttcgcaagc
    10921 aggagctgac ggtgtatacg ctgaataatg agctggaaaa tctgctgacc aacgtggtga
    10981 atcaggcgca acaggccggc aaagtgatgc ttgatagcgt gccagtggat ccgaatatgc
    11041 tcaaccagtt ccagagcacg atgcctcagg tgaaggagca gatgaaagcg gcggggaaag
    11101 atccggtgct gctggtgccg ccgcagttgc gccctttgct ggcgcgctac gcaaggttgt
    11161 ttgcgccggg gctgcatgtg ctgtcgtata acgaagtgcc ggatgagctg gagttgaaga
    11221 taatgggggc gttgatgtag gcatccggca tcggtgcata ttgccggatg cggcgtgaac
    11281 gccttatccg gcctacgggt gagcacggtt ttgtcggcat gataagacgc gtggcgtcgc
    11341 atctggcatc gg
//
LOCUS       DADSYM010000144        11330 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2415
            SAMN08039579-rid11723173.denovo.145, whole genome shotgun sequence.
ACCESSION   DADSYM010000144 DADSYM010000000
VERSION     DADSYM010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039579
            Sequence Read Archive: SRR6362533
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11330)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11330)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980162.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 03:39:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,484
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 4,347
            CDSs (with protein)               :: 4,347
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 43 of 85
            Pseudo Genes (incomplete)         :: 37 of 85
            Pseudo Genes (internal stop)      :: 19 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11330
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039579-rid11723173.denovo.145"
                     /strain="LGE2415"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8302 N 95.9628 W"
                     /collection_date="2015-08-24"
     gene            complement(170..295)
                     /locus_tag="J1Y83_003564"
                     /pseudo
     CDS             complement(170..295)
                     /locus_tag="J1Y83_003564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000450676.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1627 domain-containing protein"
     gene            337..567
                     /locus_tag="J1Y83_003565"
                     /pseudo
     CDS             337..567
                     /locus_tag="J1Y83_003565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309956.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
     gene            619..1554
                     /gene="ttcA"
                     /locus_tag="J1Y83_003566"
     CDS             619..1554
                     /gene="ttcA"
                     /locus_tag="J1Y83_003566"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine(32) synthetase TtcA"
                     /protein_id="HBA7631679.1"
                     /translation="MQENQQITKKEQYNLNKLQKRLRRNVGEAIADFNMIEEGDRIMV
                     CLSGGKDSYTMLEILRNLQQSAPINFSLVAVNLDQKQPGFPEHVLPEYLETLGVEYKI
                     VEENTYGIVKEKIPEGKTTCSLCSRLRRGILYRTATELGATKIALGHHRDDILQTLFL
                     NMFYGGKMKGMPPKLMSDDGKHIVIRPLAYCREKDIQRFADAKAFPIIPCNLCGSQPN
                     LQRQVIADMLRDWDKRYPGRIETMFSAMQNVVPSHLCDTNLFDFKGITHGSEVVNGGD
                     LAFDREEIPLQPAGWQPEEDENQLDELRLNVVEVK"
     gene            complement(1683..3056)
                     /gene="dbpA"
                     /locus_tag="J1Y83_003567"
     CDS             complement(1683..3056)
                     /gene="dbpA"
                     /locus_tag="J1Y83_003567"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase DbpA"
                     /protein_id="HBA7631680.1"
                     /translation="MTAFSTLNVLPPAQLTNLNELGYLTMTPVQAAALPAILAGKDVR
                     VQAKTGSGKTAAFGLGLLQQIDASLFQTQALVLCPTRELADQVAGELRRLARFLSNTK
                     ILTLCGGQPFGMQRDSLQHAPHIIVATPGRLLDHLQKGTVSLDALNTLVMDEADRMLD
                     MGFSDAIDDVIRFAPASRQTLLFSATWPEAIAAISGRVQRDPLAIEIDSTDALPPIEQ
                     QFYETSSKGKIPLLQRLLSLHQPSSCVVFCNTKKDCQSVCDALNEVGQSALSLHGDLE
                     QRDRDQTLVRFANGSARVLVATDVAARGLDIKSLELVVNFELAWDPEVHVHRIGRTAR
                     AGNSGLAISFCAPEEAQRANIISDMLQIKLNWQTLPANSSIVPLEAEMATLCIDGGKK
                     AKMRPGDVLGALTGDIGLDGADIGKIAVHPAHVYVAVRQAVAHKAWKQLQGGKIKGKT
                     CRVRLLK"
     gene            complement(3534..4517)
                     /gene="zntB"
                     /locus_tag="J1Y83_003568"
     CDS             complement(3534..4517)
                     /gene="zntB"
                     /locus_tag="J1Y83_003568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZntB"
                     /protein_id="HBA7631681.1"
                     /translation="MEAIKGSDVNVPDAVFAWMLDGRGGVKPLENTDVIDEAHPCWLH
                     LNYVHHDSAQWLATTPLLPNNVRDALAGESTRPRVSRLGEGTLITLRCINGSTDERPD
                     QLVAMRVYMDGRLIVSTRQRKVLALDDVVSDLEEGTGPTDCGGWLVDVCDALTDHSSE
                     FIEQLHDKIIDLEDNLLDQQIPPRGFLALLRKQLIVMRRYMAPQRDVYARLASERLPW
                     MSDDQRRRMQDIADRLGRGLDEIDACIARTGVMADEIAQVMQENLARRTYTMSLMAMV
                     FLPSTFLTGLFGVNLGGIPGGGWQFGFSIFCILLVVLIGGVALWLHRSKWL"
     gene            4772..6004
                     /gene="dgcM"
                     /locus_tag="J1Y83_003569"
     CDS             4772..6004
                     /gene="dgcM"
                     /locus_tag="J1Y83_003569"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcM"
                     /protein_id="HBA7631682.1"
                     /translation="MITHNFNTLDLLTSPVWIVSPFEEQLIYANSAARLLMQDLTFSQ
                     LRTGPYSVSSQKELPKYLSDLQNQHDIIEILTVQRKEEETALSCRLVLRELTETEPVI
                     IFEGIEAPATLGLKASRSANYQRKKQGFYARFFLTNSAPMLLIDPSRDGQIVDANLAA
                     LNFYGYNHETMCQKHTWEINMLGRRVMPIMHEISHLPGGHKPLNFVHKLADGSTRHVQ
                     TYAGPIEIYGDKLMLCIVHDITEQKRLEEQLEHAAHHDAMTGLLNRRQFYHITEPGQM
                     QHLAIAQDYSLLLIDTDRFKHINDLYGHSKGDEVLCALARTLESCARKGDLVFRWGGE
                     EFVLLLPRTPLDTALSLAETIRVSVAKVSISGLPRFTVSIGVAHHEGNESIDELFKRV
                     DDALYRAKNDGRNRVLAA"
     gene            complement(6025..6588)
                     /gene="smrA"
                     /locus_tag="J1Y83_003570"
     CDS             complement(6025..6588)
                     /gene="smrA"
                     /locus_tag="J1Y83_003570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA endonuclease SmrA"
                     /protein_id="HBA7631683.1"
                     /translation="MNLDDKSLFLDAMEDVQPLKRATDVHWHPTRNQRAPQRIDTLQL
                     DNFLTTGFLDIIPLSQPLEFRREGLQHGVLDKLRSGKYPQQASLNLLRQPVEECRKMM
                     FSFIQQALADGLRNVLIIHGKGRDDKSHANIVRSYVARWLTEFDDVQAYCTALPHHGG
                     SGACYVALRKTAQAKQENWERHAKRSR"
     gene            complement(6783..7436)
                     /locus_tag="J1Y83_003571"
     CDS             complement(6783..7436)
                     /locus_tag="J1Y83_003571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000478423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HBA7631684.1"
                     /translation="MFLGQVRGLNAEEIGFAVCTTGIFQLFSVPFYFWLSKKVNLRWL
                     LMAGLGGFVFSMYLFTPITHEWRWQELLFPQAIRGISQQFAMAPIVTLTLGGIPKERL
                     KLASGVFNLTRNLGGAIGIALCGSILNNRTNFHFSRMGEKMVSVPHAVNDFISRSALF
                     FNRSGSDQTSEILASTKLLSQLMLREAQTMAFSDTFLLISGLLFIAFLLVPAMNKSS"
     gene            complement(7327..8283)
                     /locus_tag="J1Y83_003572"
     CDS             complement(7327..8283)
                     /locus_tag="J1Y83_003572"
                     /inference="COORDINATES: protein motif:HMM:TIGR00711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHA2 family efflux MFS transporter permease
                     subunit"
                     /protein_id="HBA7631685.1"
                     /translation="MRKHIAFASMCMGLFIAQLDIQIVSSSLNEIGGGLSAGKDEMAW
                     LQTSYLIAEIIVIPLSGWLSRVFSTRWLFTLSAGIFTLMSIACGLAWNIQIMIFFRAL
                     QGVAGASMIPLVFTTAFIYYQGKELGLAAAVVSALASLSPTLGPTLGGWITDNLDWRW
                     LFYINILPGIYLVLSIPFLVNFDKPDLSLLKVADYPSIILLAMTLGCLEYTLEEGARW
                     GWLDDNTILLTSVLALVSFILFAARTLKISNPIMDLHAFKDKNFTLGCFFFWRGWNIL
                     YCIFNTCVFGAGSRIKCRRDRFCRLHNRYFPVIFCSLLLLVK"
     gene            complement(8289..9317)
                     /locus_tag="J1Y83_003573"
     CDS             complement(8289..9317)
                     /locus_tag="J1Y83_003573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000587186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HBA7631686.1"
                     /translation="MIISKKQLIGVVAIGIILAGVVFFIWWVSKGRFIQTTDDAYIGG
                     NITTVASKVSGYISAIEVRDNQSVKKGDIILRLDDRDYRANVARLEAKIKSSKANLES
                     IQATIAMQQSIIQSASETWQAVKHEEQKRLRDTERYEKLAQSAAISQQIIDNARFDYQ
                     QVAAKERKAANDFLVEKQRLAVLSAQEENVRASIEEVQAALTQALLDLEYTLVRAPID
                     GIVANRSAHTCSWVEGGTSLVSLVPVSELWVDANYKENQIAGMKPGMKAEIRADILKG
                     EVFHGHIESLSPATGASFSLIPIENATGNFTKIVQRVPVRIAFDDAKELKQLLRPGLS
                     VTVSVDER"
     gene            9414..10229
                     /locus_tag="J1Y83_003574"
     CDS             9414..10229
                     /locus_tag="J1Y83_003574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707689.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HBA7631687.1"
                     /translation="MTITLQPKASPGHHIIGLDSEHLNGGTVPWHKHLYAQLLYPAEG
                     VVRIWAGESVWLVHASSALWLPPQMPHKFVATGNVLLKTVLVSEAESETLGKVCFMTG
                     ISPLLRELLIAINQLPPSQSTTDKQQLRFSALETLILQEIKMGVKMSLELPWPNDERL
                     QQLCENLLNNQGYLPTLDNLADKINVSSRTLMRLFVKETGLTFRHWVQQMHVISAVTL
                     LDDGYSLTKIAHRLGYASAESFGNMFKRRTGYSPGKFTRRLTMHDYAITRQMI"
     gene            10619..10762
                     /locus_tag="J1Y83_003575"
                     /pseudo
     CDS             10619..10762
                     /locus_tag="J1Y83_003575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415852.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            10773..11288
                     /gene="ogt"
                     /locus_tag="J1Y83_003576"
     CDS             10773..11288
                     /gene="ogt"
                     /locus_tag="J1Y83_003576"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HBA7631688.1"
                     /translation="MLRLLEEKIATPLGPLWVICDEQFRLRAVEWEEYSERMVQLLDI
                     HYRKESYERISATNPGGLSDKLREYFAGNLSIIDTLPTATGGTPFQREVWKTLRTIPC
                     GQVMHYGQLAEQLGRPGAARAVGAANGSNPISIVVPCHRVIGRNGTMTGYAGGVQRKE
                     WLLRHEGYLLL"
BASE COUNT         3103 a         2821 c         2545 g         2861 t
ORIGIN      
        1 aaacacattc atatctaatg taatatttcc atgcgcaaat ataaccacca accatactca
       61 tcatttatta cagagaatta ttttattcac cgaaaagttt cacaaaatat cccctgatag
      121 aaaactaaaa aacactgcat aatatcgcta ttctggcact cgcgaaaaat tatattctgc
      181 acctgttgta gcgttctatt tctccgcctg gcaacgaatg cacaatgtcc gccacagttt
      241 ctgttctaat ttcttcctgt gttaccgcat cctgtacggg tagcgcaaca ccagcatgct
      301 aagcaaggct ttcccatcag atttcactac gactatttgg gagcgagatg gaatgcagca
      361 gtaaaacgtg ctggtattcg ccgccgtaat ccgtaccata cgcgacatac ttttgcctgc
      421 tggctgttga cggcaggagc gaacccggca tttatagcca gccagatggg gcatgaaact
      481 gcgcagatgg tgtatgaaat ttacggtatg tggattgatg acatgaacga cgaacaggta
      541 gccatgttga atgcgcggtt atcgtagttg caaagtttgc cctcaatatg ccccatttag
      601 taccagagaa ctgaaataat gcaagaaaat caacaaatta caaagaaaga acaatacaac
      661 ctgaacaaat tacaaaaacg tctgcgtcgt aacgtgggcg aagccattgc tgacttcaat
      721 atgattgaag aaggcgatcg catcatggtt tgcctctccg ggggtaaaga cagctatacc
      781 atgctggaaa ttctgcgcaa tttgcagcaa agcgcgccaa tcaatttttc gttggtggct
      841 gttaacctcg atcaaaagca accgggcttc ccggaacacg ttctgcccga gtatcttgaa
      901 acgctgggcg ttgagtacaa gattgttgaa gagaacactt acggtatcgt gaaagagaag
      961 attccggagg gcaaaaccac ttgctcactg tgttctcgtc ttcgtcgcgg tatcctttat
     1021 cgcaccgcaa cggaactggg ggcgacgaag atcgcgctgg gtcaccatcg tgacgatatc
     1081 ctgcaaacgc tgttcttaaa tatgttctac ggtggcaaga tgaaaggtat gcctccgaaa
     1141 ctgatgagcg atgatggcaa acatatcgtg attcgtccgc tggcctactg ccgcgagaaa
     1201 gatattcagc gctttgccga tgcaaaagcg ttcccgatta ttccgtgcaa cctgtgcggt
     1261 tctcagccta acctgcaacg tcaggtaatt gctgacatgt tgcgtgactg ggataaacgt
     1321 tatccggggc gtatcgagac gatgttcagc gcgatgcaga atgtggtgcc gtcgcatctg
     1381 tgcgatacca acctgttcga tttcaaaggc attacccacg gttctgaagt ggttaacggg
     1441 ggtgatctgg cgtttgatcg cgaagagatc ccactacaac cagcgggctg gcagccagaa
     1501 gaagatgaaa atcagttgga tgagttacgg ctgaatgtgg ttgaagtgaa ataaccagga
     1561 tagcgcccga tacgcaagcg tatcgggcta ctcttatgga ggccggataa gacgcggcca
     1621 gcgtcgcatc cggcaatgcc taagaagatg tttactcttg caccctgcaa ttcaacattt
     1681 cattatttta ataaccgcac ccggcacgtt tttcctttaa tcttcccgcc ctgtaactgt
     1741 ttccatgctt tatgagcaac agcctgacgc accgcgacat aaacatgcgc cggatgcacg
     1801 gcgattttgc caatatctgc gccatcaagc ccgatgtctc ctgtcagcgc acctaataca
     1861 tcacccgggc gcattttggc ttttttcccg ccatcgatac acaacgttgc catttctgct
     1921 tccagcggca caatggaact attcgctggc agcgtttgcc agttaagttt tatctgcaac
     1981 atgtcagaaa tgatattggc ccgctgtgct tcttccgggg cacagaaact gatcgccaga
     2041 ccgctatttc ctgcacgagc tgtacgaccg atgcgatgta catgaacttc agggtcccac
     2101 gccagctcaa agttcaccac cagctcaagc gattttatat ccagaccacg cgcagcaaca
     2161 tcagtcgcga ccagtacacg ggcgctaccg ttagcaaaac gtaccagggt ctgatcgcga
     2221 tcgcgttgct ccagatcgcc gtgtaacgac aatgcacttt gccctacttc attcagcgcg
     2281 tcgcagacag actggcaatc ttttttggta ttgcaaaaca ccacgcaaga ggatggctga
     2341 tgcaagctta ataaccgttg caacagagga attttgcctt tgctggatgt ctcataaaat
     2401 tgttgttcaa taggtggcag agcatctgtt gagtcaattt caatcgctaa aggatcgcgt
     2461 tgcactcttc cgctgattgc ggcaatggct tccggccagg ttgccgaaaa cagaagtgtc
     2521 tgccgagatg ctggcgcaaa acggatgaca tcatcaatgg cgtcactaaa tcccatatcc
     2581 agcatgcggt cggcctcatc catcaccagc gtattcaacg catccagtga tacagtgcct
     2641 ttttgcaggt gatccagcaa acggcccggc gttgccacga taatatgcgg cgcatgttgt
     2701 aacgaatcac gctgcatacc gaacggttga ccaccgcaca acgtcaaaat tttggtattt
     2761 gacagaaaac gcgccagccg acgcaattca cctgccacct gatccgccag ttcacgcgta
     2821 ggacacagca ctaaagcctg ggtttgaaat agcgacgcat caatttgctg taacaagccg
     2881 aggccaaaag ccgccgtttt gccgctgccg gttttcgcct gcacgcgaac atcttttccg
     2941 gcaaggatcg ccggaagcgc ggcggcctgc accggcgtca tggttaaata acccaactca
     3001 ttaaggtttg tgagttgggc gggaggcaaa acattcaggg tagaaaaagc ggtcacaatc
     3061 tattctcgtg gtcatcgacg caaagtcagc aggcgcgtat cctcgcagat ctacgctcac
     3121 gatgcgacaa tttaatcggt tcttcatcgg gtggtgggtc aggcatgggt tgcgggcgag
     3181 ggatcggatc aggtactgga acaggatcgc caggaatcgg ttcagggaca ggaatttgca
     3241 aataaataag tgtcgtcata tttccctccg gtcattgggt ggactcttaa agggtagacg
     3301 ctgataaata acaggcaaaa aaaagccgac tcatcaaagt cggcgtcgta cgaatcaatt
     3361 gtgctatgca gtaattcaaa aaaggaagta agacaatatg gagcgcaacg cccatcgctt
     3421 gacgttgcat tcacctgcaa gagagatatt gccctgaatg ggtagagagt ttattgactt
     3481 cgctcaaact ttacggcgtt tttgtataca gacagccgga aaaattgctt ttgttacaac
     3541 catttactac gatgcaacca taaagcaaca ccaccaataa gaacaactaa cagaatacaa
     3601 aaaattgaaa atccaaattg ccatccgccg ccagggatcc caccaaggtt gacgccaaat
     3661 aaccctgtca gaaacgtact gggtaaaaag accattgcca tcaacgacat tgtataggta
     3721 cgacgagcta aattttcctg catcacctga gcgatttcat ccgccatcac gccagtccgt
     3781 gctatacagg cgtcgatttc gtcaagaccg cgcccaaggc gatcggcaat atcctgcatc
     3841 cgacggcgtt ggtcatcgct catccacggc aaacgttcac tggcaagacg agcataaaca
     3901 tcacgttgcg gtgccatata gcgacgcatc acaattaatt gtttgcgcag cagagccagg
     3961 aatccacgcg gtggaatttg ctgatcgaga agattatctt cgaggtcgat aattttatcg
     4021 tgcagctgct cgataaattc gctggaatga tcggtcaacg catcgcacac atcaaccagc
     4081 caccccccgc aatcggtcgg accagtgccc tcttccagat cgctcaccac atcgtccagc
     4141 gccagcactt tgcgttgtcg ggtcgaaaca attaatcgcc catccatgta tacacgcatg
     4201 gcaaccagct gatcggggcg ttcatcggtg ctgccgttta tacagcgcaa tgtaatcagc
     4261 gtgccttcac cgagacggct gactcggggc cgtgtgctct cgcccgccag cgcatcacgt
     4321 acgttattgg gaagcagcgg tgttgtcgcc agccattggg cgctatcatg gtgtacataa
     4381 ttaaggtgga gccagcaggg atgcgcttca tcaatcacat ctgtattttc cagcggttta
     4441 acgccgcctc taccatccag catccatgca aatactgcat ccgggacatt aacgtccgat
     4501 cccttaatcg cttccacagt gcctccatca tcaacgcatt attttgtagt ctagccttct
     4561 ggccctgtta cgcaacatct catcacccca ttaccctgaa atgattaata aaattctgtc
     4621 taaattgaat acaaaaagca aaatgctttt ccgtatacaa accgtgtgaa gtgttaaata
     4681 gcgtctatca ttatcagaat tatctgatca tatgacgtgg cttttttgcg atcggatagc
     4741 aacaaaaatt gataaaaata atgggatctc aatgattaca cacaacttca ataccctgga
     4801 cttactcacc agtcctgtct ggatcgtttc gccctttgag gaacagttaa tttatgccaa
     4861 cagcgcggcg cgactgttga tgcaagacct cacgtttagt cagctacgaa ccggtcccta
     4921 ttccgtctcc tcacaaaaag aactgccgaa atacctctcc gatctgcaaa atcaacacga
     4981 tattatcgaa atcctcaccg ttcagcgtaa agaagaggaa acagcattaa gctgtcggct
     5041 tgttttgcga gagctgacag aaacagaacc ggtgattatt ttcgaaggta tcgaagcacc
     5101 ggcaacgctg ggtttaaaag ccagtcgctc ggcaaattat cagcgcaaaa aacaaggttt
     5161 ttatgcgcgc ttttttctga ctaactctgc accaatgttg ttgattgacc cctcacgaga
     5221 tggccaaatc gtcgatgcta acctcgccgc gctcaatttc tatggttata accatgaaac
     5281 gatgtgccag aaacatacct gggaaattaa tatgctcggg cgtcgcgtca tgcctatcat
     5341 gcatgaaatc tcgcatttac ccggtggtca taaacctttg aattttgttc ataaactggc
     5401 ggatggttcg actcgtcatg tgcagaccta tgccgggccg attgaaattt atggcgacaa
     5461 gctcatgtta tgtattgtgc atgatattac tgagcaaaaa cggctggagg agcagctgga
     5521 acatgctgcc caccatgatg cgatgaccgg attactgaat cggcgacagt tttatcacat
     5581 taccgagcca ggccaaatgc agcacctcgc tatcgctcag gattacagct tgttgctcat
     5641 cgacaccgat cgttttaaac acattaacga tctctatggg cattctaaag gtgatgaggt
     5701 gttatgcgcc cttgcccgca ctctcgaaag ttgcgctcgc aaaggcgatt tggtgtttcg
     5761 ttggggaggc gaagagtttg tcttattgct accaagaacc ccactggata ccgcgctttc
     5821 gctggctgaa actatccgcg taagcgtggc aaaagtgagt atttcgggct taccacgctt
     5881 tactgtcagc attggtgtgg cgcatcacga aggaaatgaa agcatcgatg agctgtttaa
     5941 acgcgttgat gatgctttgt atcgagcgaa aaatgatgga cgcaaccgcg tgctggcggc
     6001 ataagccacg gatgcgtctc gtgatcaacg actgcgctta gcgtggcgct cccagttttc
     6061 ttgcttcgcc tgcgctgttt tacgtagtgc gacgtaacac gccccgctgc cgccatgatg
     6121 cggtagcgca gtgcaatatg cctgaacatc atcaaattcg gtcagccagc gcgccacata
     6181 gctgcggaca atattggcat gcgatttatc atcccgtcct ttgccatgaa taatcagcac
     6241 gttacgcaaa ccatccgcca gggcttgttg aatgaaactg aacatcattt tgcggcactc
     6301 ttccaccggc tggcgcaaaa gattcaggct cgcctgttgc ggatatttac cactgcgcag
     6361 cttatccagc accccatgtt gcaaaccttc ccgccgaaac tccagcggct gacttagcgg
     6421 gatgatgtcg agaaatccag tggtgaggaa attatcaagt tgcagcgtgt cgatacgctg
     6481 cggcgcacgt tggttacgcg ttggatgcca gtggacatct gtagcacgtt tcagcggctg
     6541 gacatcttcc atggcgtcaa gaaacagcga tttgtcgtca aggttcatgt aacctcctat
     6601 gcaccttcac acgtcgtgat tcagacgggt taattgttca tacttactgc atcgttccct
     6661 tcacagattg aaacaatact ctacttcata cctgtgagta tctgcgcagg cggggcattt
     6721 tatactagcc tgttagcagt atcgaacagt ttagcccgag tttaattcag cacaggctcc
     6781 gatcaggatg atttattcat agcaggaacc aacaaaaatg cgataaataa cagaccactg
     6841 attagcagaa aggtatccga aaatgccata gtttgcgcct ccctcagcat caattgactc
     6901 aataatttgg ttgaagccag tatttccgat gtctggtcac ttccactgcg attaaaaaac
     6961 aaagcacttc tggaaatgaa gtcattcacc gcatggggaa cagaaaccat tttttcaccc
     7021 attctcgaaa aatgaaaatt agttcgatta ttgagaatag agccacacag ggcaatacca
     7081 attgcgcccc caagattacg tgttaaatta aatacaccag aggctaactt cagccgttct
     7141 tttggaattc cccccagcgt taatgttacg atgggggcca tcgcaaactg ctgcgaaatc
     7201 cctctaattg cctgtggaaa cagtaactcc tgccatctcc attcgtgggt tataggggta
     7261 aaaagataca ttgaaaacac aaatccaccc agccccgcca tcaacagcca tcgcagattt
     7321 acttttttac ttaaccagaa gtaaaaggga acagaaaata actggaaaat acctgttgtg
     7381 caaacggcaa aacctatctc ttcggcattt aatccgcgaa cctgccccaa aaacacaggt
     7441 attaaatata cagtagagaa tattccaacc cctccagaaa aagaaacatc ccagtgtgaa
     7501 atttttatcc ttaaaagcat gaagatccat tatcggattg gatattttaa gtgtacgcgc
     7561 agcaaataaa ataaaactta ccaatgccag aactgatgtc agcaaaatag tattatcatc
     7621 cagccatccc cagcgcgctc cttcttcaag ggtatattcc agacatccca gtgtcatggc
     7681 caacaggata atactcggat aatcagcaac tttcagtaaa gagagatcag gtttatcaaa
     7741 attaaccaga aaaggaatac tcaaaactaa atatatgccc ggtaatatat tgatataaaa
     7801 tagccatcgc cagtcgaggt tatcagtgat ccagcccccc agagttggcc ctagcgtcgg
     7861 tgacagtgag gccagcgcac tgactaccgc agcagccaac cccaactctt taccctggta
     7921 ataaataaac gccgtcgtga aaactaaagg gatcatggat gcgccagcaa ctccctgtaa
     7981 agcacgaaaa aaaatcatga tctgaatatt ccatgcaagt ccacaagcga tactcattaa
     8041 agtaaatatt cccgcggata gagtaaaaag ccaacgagtg gaaaataccc gtgacagcca
     8101 cccagagagc ggaataacaa taatctcagc aattaaatag ctggtctgaa gccaggccat
     8161 ttcgtcttta cctgcggaca gaccaccacc tatttcattt agggatgacg atacaatttg
     8221 aatatcaagc tgagcaataa ataatcccat acacatactg gcgaaagcaa tatgtttacg
     8281 catagacatc atctctcatc aacactgacg gtgacggaca gtcccgggcg aagaagttgc
     8341 tttaattcct ttgcgtcgtc aaatgcaatc cgtaccggga cgcgctgaac aattttggtg
     8401 aaattgccag tggcattttc tatcggaatc aagctgaaac tggctcctgt cgctggggaa
     8461 aggctttcaa tgtgaccgtg aaacacttcc cctttgagaa tatcagcacg gatctcagcc
     8521 ttcattcctg gcttcattcc cgctatttgg ttttctttat aatttgcatc aacccacaac
     8581 tcactgactg gtactagtga aactaacgat gtgccgcctt ccacccaact gcatgtatgg
     8641 gcgctgcggt ttgcgacaat gccatcaatc ggcgcacgaa caagggtata ttccagatcc
     8701 agtagtgcct gagtcaaagc ggcctgaact tcttcgatac ttgcacgtac gttttcttcc
     8761 tgagcagata aaactgccag ccgttgcttc tccaccagga aatcgttcgc ggctttgcgc
     8821 tcctttgctg cgacctgctg gtagtcaaac ctggcgttat caataatttg ttgcgatatt
     8881 gccgctgatt gtgcgagttt ttcataacgt tcagtatctc gcaggcgctt ttgctcctca
     8941 tgttttactg cctgccatgt ttcactggca ctttgaatta tcgactgctg catcgcaata
     9001 gtggcttgaa tgctttcgag attggctttg ctacttttta tttttgcctc aagcctggcg
     9061 acatttgcac gataatcccg atcatcgaga cgcaggataa tatcgccttt ttttacactc
     9121 tgattatcgc gcacctctat agcagaaata taaccagaaa ctttacttgc caccgtggtg
     9181 atattaccac caatgtaagc atcatccgtc gtctgtataa atcttccttt gcttacccac
     9241 cagataaaaa agacaacgcc agccaaaata ataccgatcg ctaccacccc aataagctgt
     9301 tttttagaaa ttatcatcac tctttacacc gtatatatcc ggaaacttgc atacgtcata
     9361 tttaaaattt aacatggctt atttgatgat ttctgacaaa atacctactg taaatgacaa
     9421 tcacactgca acccaaagca tcaccagggc atcacattat cggactggat tcggaacatc
     9481 tgaatggagg cacagttccc tggcataaac atctctatgc ccagttgctt tacccggctg
     9541 aaggagtcgt tcgtatatgg gctggagaaa gcgtctggct agtgcatgcc agcagcgcgc
     9601 tttggctgcc cccacaaatg ccgcataagt ttgttgcgac aggcaacgta cttttaaaaa
     9661 ccgtactggt ttcagaagcg gaaagtgaaa cgctgggaaa agtttgcttc atgacgggta
     9721 tttcacccct gttaagggaa ttactaattg caattaacca acttccaccg tcgcaaagta
     9781 ccacagataa acaacagtta cgtttttcag ccctcgagac actgatactt caggaaataa
     9841 aaatgggggt gaaaatgtca cttgaactcc cttggccaaa tgatgagcgg ctacagcagc
     9901 tttgtgagaa tttgctaaac aaccaagggt atctgccaac actggataat ctagcggata
     9961 aaattaacgt cagtagccgt acactcatgc gcttatttgt taaagagacg ggattaacat
    10021 ttcgccactg ggttcagcaa atgcacgtta tttctgcagt gacgcttctt gatgatggat
    10081 attcgttgac caaaattgcc catcgtctgg gctatgcctc agctgaatcg ttcggcaata
    10141 tgtttaagcg tcgaacgggc tactcacccg gtaaatttac tcgtcgatta acgatgcatg
    10201 attatgcaat cacaagacaa atgatttaac ttatattttc gtgaaatctg tcactgaaga
    10261 aaattggcaa cggatgggta aaaccgttat aacactgtca ccggtcacca ggaccccagg
    10321 ccggatttaa gacgaggatg cactgctgtg tgtactgtag agtctggcgg atgtcgacag
    10381 gctctatttt tttattcatt ttttagccct gtaaataacc acattctcgc cgggattggg
    10441 gaatgagtga cgagatcagt ggtgtctggc ggctatcacg ccacagaatc aaataacaga
    10501 tagcctacgg taaaatttcg ctgactggaa ttatcccacc ctcgatccat gtgatggctt
    10561 gccctcattt cttcaggcag aaaaatcaaa aaatcgcttt atgctatcag actgcgacaa
    10621 accagacgcg aaactgggta cttactattc gttagtctta ccctatccac ttatcttttt
    10681 ggtggtatgg ctgctgatgt tgctggcgtg gtatcttgtt ggcttgccga caggtccggg
    10741 tatttaccca cgtttgtctt aagagagaac ggatgctgag attacttgaa gaaaaaattg
    10801 ccacgccact gggtccactg tgggtgattt gcgatgagca atttcgcctg cgggcggttg
    10861 aatgggaaga gtacagcgaa cgcatggtgc agctgctgga catccattat cgcaaagaaa
    10921 gctatgagcg catttctgcc accaacccag gtggtttaag cgacaagctt cgtgaatatt
    10981 ttgccggtaa tcttagcatt attgatacgc ttcccactgc cacggggggg acgccatttc
    11041 agcgcgaagt ctggaaaaca ctgcgcacta tcccctgcgg gcaggtaatg cattatggcc
    11101 aactggctga acaattaggc cgccctggcg cggcgcgtgc cgttggtgcg gcaaacggat
    11161 cgaatcccat cagcatcgtc gtaccttgcc atcgggttat tggccgaaac ggcaccatga
    11221 ccggatatgc aggcggagtt cagcgaaaag agtggttatt gcgccacgaa ggttatcttt
    11281 tgctgtaaac attaaacaat ttgtgccagc ttgttcacac ttttatgtaa
//
LOCUS       DADSYM010000145        11283 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2415
            SAMN08039579-rid11723173.denovo.146, whole genome shotgun sequence.
ACCESSION   DADSYM010000145 DADSYM010000000
VERSION     DADSYM010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039579
            Sequence Read Archive: SRR6362533
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11283)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11283)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980162.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 03:39:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,484
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 4,347
            CDSs (with protein)               :: 4,347
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 43 of 85
            Pseudo Genes (incomplete)         :: 37 of 85
            Pseudo Genes (internal stop)      :: 19 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11283
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039579-rid11723173.denovo.146"
                     /strain="LGE2415"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8302 N 95.9628 W"
                     /collection_date="2015-08-24"
     gene            191..295
                     /locus_tag="J1Y83_003577"
     CDS             191..295
                     /locus_tag="J1Y83_003577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001312672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2618 domain-containing protein"
                     /protein_id="HBA7631689.1"
                     /translation="MENNNRLMPHIRRTTHIMKFAHRNSFDFHFFNAR"
     gene            605..2803
                     /gene="speF"
                     /locus_tag="J1Y83_003578"
     CDS             605..2803
                     /gene="speF"
                     /locus_tag="J1Y83_003578"
                     /EC_number="4.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine decarboxylase SpeF"
                     /protein_id="HBA7631690.1"
                     /translation="MSELKIAVSRSCPDCFSTHRECVNIDKSNYIDVAAIILSVNDVE
                     RGKLDEIDATGYGIPVFIATENEERVPAEYLPRISGVFEHCESRKEFYGRQLETAASH
                     YETQLRPPFFRALVDYVNQGNSAFDCPGHQGGEFFRRHPAGNQFVEYFGEMLFRSDLC
                     NADVAMGDLLIHEGAPCIAQQHAAKVFNADKTYFVLNGTSSSNKVVLNALLTPGDLVL
                     FDRNNHKSNHHGALLQAGATPVYLETARNPYGFIGGIDAHCFEESYLRELITEVAPQR
                     AKEARPFRLAVIQLGTYDGTIYNARQVVDKIGHLCDYILFDSAWVGYEQFIPMMADCS
                     PLLLELNENDPGILVTQSVHKQQAGFSQTSQIHKKDSHIKGQQRYVPHKRMNNAFMMH
                     ASTSPFYPLFAALDINAKMHEGVSGRNMWMDCVVNGINARKLILDNCQHIRPFVPELV
                     DGKPWQSYETAQIAVDLRFFKFVPGEHWHSFEGYAENQYFVDPCKLLLTTPGIDARNG
                     EYEAFGVPATILANFLRENGVVPEKCDLNSILFLLTPAEDMAKLQQLVALLVRFEKLL
                     EADAPLAEVLPSIYKQHEERYAGYTLRQLCQEMHDLYARHNVKQLQKEMFRKEHFPRV
                     SMNPQEANYAYLRGEVELVRLPDAEGRIAAEGALPYPPGVLCVVPGEIWGGAVLRYFS
                     ALEEGINLLPGFAPELQGVYIEEHDGRKQVWCYVIKPRDAQSTLLKGEKL"
     gene            2800..4119
                     /gene="potE"
                     /locus_tag="J1Y83_003579"
     CDS             2800..4119
                     /gene="potE"
                     /locus_tag="J1Y83_003579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine-ornithine antiporter"
                     /protein_id="HBA7631691.1"
                     /translation="MSQAKSNKMGVVQLTILTMVNMMGSGIIMLPTKLAEVGTISIIS
                     WLVTAVGSMALAWAFAKCGMFSRKSGGMGGYAEYAFGKSGNFMANYTYGVSLLIANVA
                     IAISAVGYGTELLGASLSPVQIGLATIGVLWICTVANFGGARITGQISSITVWGVIIP
                     VVGLCIIGWFWFSPTLYVDSWNPHHAPFFSAVGSSIAMTLWAFLGLESACANTDVVEN
                     PERNVPIAVLGGTLGAAVIYIVSTNVIAGIVPNMELANSTAPFGLAFAQMFTPEVGKV
                     IMALMVMSCCGSLLGWQFTIAQVFKSSSDEGYFPKIFSRVTKVDAPVQGMLTIVIIQS
                     GLALMTISPSLNSQFNVLVNLAVVTNIIPYILSMAALVIIQKVANVPPSKAKVANFVA
                     FVGAMYSFYALYSSGEEAMLYGSIVTFLGWTLYGLVSPRFELKNKHG"
     gene            complement(4176..5816)
                     /gene="pgm"
                     /locus_tag="J1Y83_003580"
     CDS             complement(4176..5816)
                     /gene="pgm"
                     /locus_tag="J1Y83_003580"
                     /EC_number="5.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-glucose phosphate-specific
                     phosphoglucomutase"
                     /protein_id="HBA7631692.1"
                     /translation="MAIHNRAGQPAQQSDLINVAQLTAQYYVLKPEAGNAEHAVKFGT
                     SGHRGSAARHSFNEPHILAIAQAIAEERAKNGITGPCYVGKDTHALSEPAFISVLEVL
                     AANGVDVIVQENNGFTPTPAISNAILVHNKKGGPLADGIVITPSHNPPEDGGIKYNPP
                     NGGPADTNVTKVVEDRANALLADGLKGVKRISLDEAMASGHVKEQDLVQPFVEGLADI
                     VDMAAIQKAGLTLGVDPLGGSGIEYWKRIGEYYNLNLTIVNDQVDQTFRFMHLDKDGA
                     IRMDCSSECAMAGLLALRDKFDLAFANDPDYDRHGIVTPAGLMNPNHYLAVAINYLFQ
                     HRPQWGKDVAVGKTLVSSAMIDRVVNDLGRKLVEVPVGFKWFVDGLFDGSFGFGGEES
                     AGASFLRFDGTPWSTDKDGIIMCLLAAEITAVTGKNPQEHYNELAKRFGAPSYNRLQA
                     AATSAQKAALSKLSPEMVSASTLAGDPITARLTAAPGNGASIGGLKVMTDNGWFAARP
                     SGTEDAYKIYCESFLGEEHRKQIEKEAVEIVSEVLKNA"
     gene            complement(5842..6387)
                     /gene="seqA"
                     /locus_tag="J1Y83_003581"
     CDS             complement(5842..6387)
                     /gene="seqA"
                     /locus_tag="J1Y83_003581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation negative regulator SeqA"
                     /protein_id="HBA7631693.1"
                     /translation="MKTIEVDDELYSYIASHTKHIGESASDILRRMLKFSAASQPAAP
                     VTKEVRVASPAIVEAKPVKTIKDKVRAMRELLLSDEYAEQKRAVNRFMLLLSTLYSLD
                     AQAFAEATESLHGRTRVYFAADEQTLLKNGNQTKPKHVPGTPYWVITNTNTGRKCSMI
                     EHIMQSMQFPAELIEKVCGTI"
     gene            6572..7336
                     /gene="ybfF"
                     /locus_tag="J1Y83_003582"
     CDS             6572..7336
                     /gene="ybfF"
                     /locus_tag="J1Y83_003582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HBA7631694.1"
                     /translation="MKLNIRAQTAQNQHNNSPIVLVHGLFGSLDNLGVLARDLVNDHN
                     IIQVDMRNHGLSPRDPVMNYPAMAQDLVDTLDAQQIDKATFIGHSMGGKAVMALTALA
                     PDRIDKLVAIDIAPVDYHVRRHDEIFAAINAVSESDAQTRQQAAAIMRQHLNEEGVIQ
                     FLLKSFVDGEWRFNVPVLWDQYPHIVGWEKIPAWDHPALFIPGGNSPYVSEQYRDDLL
                     AQFPQARAHVIAGAGHWVHAEKPDAVLRAIRRYLND"
     gene            7407..7769
                     /gene="ybfE"
                     /locus_tag="J1Y83_003583"
     CDS             7407..7769
                     /gene="ybfE"
                     /locus_tag="J1Y83_003583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LexA regulated protein"
                     /protein_id="HBA7631695.1"
                     /translation="MYYGALSIRAGAWLIVSPEVTKIMAKEQTDRTTLDLFAHERRPG
                     RPKTNPLSRDEQLRINKRNQLKRDKVRGLKRVELKLNAEAVEALNELAESRNMSRSEL
                     IEEMLMLQLAALRSQGIV"
     gene            7909..8439
                     /gene="fldA"
                     /locus_tag="J1Y83_003584"
     CDS             7909..8439
                     /gene="fldA"
                     /locus_tag="J1Y83_003584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin FldA"
                     /protein_id="HBA7631696.1"
                     /translation="MAITGIFFGSDTGNTENIAKMIQKQLGKDVADVHDIAKSSKEDL
                     EAYDILLLGIPTWYYGEAQCDWDDFFPTLEEIDFNGKLVALFGCGDQEDYAEYFCDAL
                     GTIRDIIEPRGATIVGHWPTAGYHFEASKGLADDDHFVGLAIDEDRQPELTAERVEKW
                     VKQISEELHLDEILNA"
     gene            8649..8735
                     /gene="uof"
                     /locus_tag="J1Y83_003585"
     CDS             8649..8735
                     /gene="uof"
                     /locus_tag="J1Y83_003585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001491546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fur leader peptide"
                     /protein_id="HBA7631697.1"
                     /translation="MIRIISRANSVTSSNEVNRLVTGQIPHD"
     gene            8728..9174
                     /gene="fur"
                     /locus_tag="J1Y83_003586"
     CDS             8728..9174
                     /gene="fur"
                     /locus_tag="J1Y83_003586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric iron uptake transcriptional regulator"
                     /protein_id="HBA7631698.1"
                     /translation="MTDNNTALKKAGLKVTLPRLKILEVLQEPDNHHVSAEDLYKRLI
                     DMGEEIGLATVYRVLNQFDDAGIVTRHNFEGGKSVFELTQQHHHDHLICLDCGKVIEF
                     SDDSIEARQREIAAKHGIRLTNHSLYLYGHCAEGDCREDEHAHEGK"
     gene            complement(9258..9584)
                     /locus_tag="J1Y83_003587"
     CDS             complement(9258..9584)
                     /locus_tag="J1Y83_003587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA7631699.1"
                     /translation="MKKLILIAMMASGLVACAQSTAPQEDSRLKEAYSACINTAQGSP
                     EKIEACQSVLNVLKKEKQHQQFAEQESVRVLDYQQCIQATQTGNDQAVKADCDKVWQE
                     IRSNNK"
     gene            complement(9634..11040)
                     /gene="chiP"
                     /locus_tag="J1Y83_003588"
     CDS             complement(9634..11040)
                     /gene="chiP"
                     /locus_tag="J1Y83_003588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chitoporin"
                     /protein_id="HBA7631700.1"
                     /translation="MRAFSGKRSTLALAIAGVTAMSGFMAIPEARAEGFIDDSTLTGG
                     IYYWQRERDRKDVTDGDKYKTNLSHSTWNANLDFQSGYAADMFGLDIAVFTAIEMAEN
                     GDSSHPNEIAFSKSNKAYDEDWSGDKSGISLYKAAAKFKYGPVWARAGYIQPTGQTLL
                     APHWSFMPGTYQGAEAGANFDYGDAGALSFSYMWTNEYKAPWHLEMDEFYQNDKTTKV
                     DYLHSLGAKYDFKNNFILEAAFGQAEGYIDQYFAKASYKFDIAGSPLTTSYQFYGTRD
                     KVDDRSVNDLYDGTAWLQALTFGYRAADVVDLRLEGTWVKADGQQGYFLQRMTPTYAS
                     SNGRLDIWWDNRSDFNANGEKAVFFGAMYDLKNWNLPGFAIGASYVYAWDAKPATWQS
                     NPDAYYDKNRTIEESAYSLDAVYTIQDGRAKGTMFKLHFTEYDNHSDIPSWGGGYGNI
                     FQDERDVKFMVIAPFTIF"
BASE COUNT         2771 a         2803 c         2776 g         2933 t
ORIGIN      
        1 cttaatgaaa atacaaccca caaaattgca acatttaaac aacaaaacag catctaatat
       61 ggcgctattc atcattaatg atttattatt tgggatatga tcgttttttg ttgatcttct
      121 tcacagatta tagctatttc atggatagaa taaccctacc ttcaactgac acagaaaaag
      181 gtaaaggtaa atggaaaata ataaccgctt aatgcctcat ataaggcgga caacccatat
      241 catgaagttt gcccatcgta atagttttga ctttcatttc tttaatgccc gctagtcttc
      301 tgactaaagg gcaccccagc gtacaggtct ccctagcttt aagcattaca ggttaatacc
      361 tgtatccctc ggtgctcata tactgctaac ccttttaacc tcttaataat tcgagtcgca
      421 gcataggcaa cttgaagtat gacgagtata gccagttacc gggctggtct gggttattgc
      481 atctgcaaaa agcaaactac tgatttattt atcagcggtg gggttttgct tttttccggc
      541 gtgatcaatt tctcctttga gaaattgagg acctgctatt acctgaaata aagagatgaa
      601 caaaatgtca gaattaaaaa ttgccgttag ccgttcttgc ccggattgtt tttccactca
      661 tcgtgaatgc gtgaatattg acaaaagtaa ttatattgac gttgccgcca ttattttatc
      721 agtcaatgat gttgaacgtg gaaaactcga tgaaatagac gctactggct atggcattcc
      781 tgtttttatt gcaacggaaa atgaagaacg tgtaccagca gaatatctgc cacgtatttc
      841 tggtgtcttc gagcattgcg aatcacggaa agagttttat ggacgtcagt tagaaaccgc
      901 tgccagccat tatgaaacgc aactgcgccc gcccttcttc cgcgcactgg tcgattatgt
      961 caatcagggc aatagcgctt ttgactgccc gggtcatcag ggaggtgagt ttttccgtcg
     1021 ccatccggca ggcaatcagt tcgtggaata ttttggcgaa atgctcttcc gctccgactt
     1081 gtgtaatgcc gacgttgcaa tgggcgatct actgattcat gaaggtgctc catgcattgc
     1141 acagcaacat gcggctaaag tgtttaatgc cgataaaacg tactttgttt taaacggcac
     1201 ctcctcctcc aacaaagtgg ttttaaacgc cctgctgaca ccgggtgatc tggtgctgtt
     1261 tgaccgcaat aaccataagt ctaaccatca cggggccttg ctacaggctg gtgcaacacc
     1321 ggtttatctg gaaacagcac gtaacccgta tggttttatc ggtggcattg atgcgcactg
     1381 cttcgaagaa agttatctgc gtgaactgat tacagaagtt gcaccgcaac gggcaaaaga
     1441 agcgcgtccg ttccgcctcg ctgtgattca gttaggcacc tacgacggta ctatttataa
     1501 cgcccgccag gtggtggata aaatcggtca tctgtgtgac tacatcctgt ttgactcagc
     1561 atgggtcggc tatgaacagt ttattccgat gatggcggac tgttcgccgc tgctgctgga
     1621 gcttaatgag aacgatccgg gtattctggt tacgcaatct gtgcataaac aacaggctgg
     1681 tttttctcag acttcacaaa ttcataaaaa agacagccat atcaaagggc aacagcgtta
     1741 tgtaccgcac aaacgcatga acaacgcctt tatgatgcac gcctccacca gcccgttcta
     1801 tccgctgttt gccgcactgg atatcaacgc caaaatgcat gaaggtgtca gcggtcgtaa
     1861 tatgtggatg gattgtgtgg ttaacggtat taatgcccgc aaattgatcc tcgataactg
     1921 ccagcacatt cgcccgttcg ttcctgaact ggtggatggt aaaccctggc agtcgtatga
     1981 aacagcgcaa attgcggttg atctgcgctt ctttaaattt gtacccggtg agcactggca
     2041 ttcttttgaa ggctacgcag agaaccaata ctttgtcgat ccgtgcaaac tgttgctgac
     2101 aactccaggt attgatgcac gtaacggcga atatgaagcg ttcggtgtac ccgcaacgat
     2161 tcttgctaac ttcctgcgcg aaaatggcgt agtgccggaa aaatgcgatc ttaactccat
     2221 cctcttcctg ctgactccgg cagaagatat ggccaaactg cagcaacttg ttgccctgct
     2281 ggtacgcttc gaaaaactac tggaagctga tgcgcctctg gcggaagtac tgccttccat
     2341 ctacaaacag catgaagagc gctacgccgg ttataccctt cgtcagttgt gccaggaaat
     2401 gcatgatttg tatgcccgcc acaacgtgaa acaactgcaa aaagagatgt tccgtaagga
     2461 acacttccca cgcgtcagca tgaatccgca agaagccaac tacgcctact tacgcggtga
     2521 agtggagctg gttcgtctgc cggatgcaga aggccgtatc gctgccgaag gtgcgcttcc
     2581 ttatcctccg ggtgtgctgt gtgttgttcc tggtgaaatc tggggtggtg ctgttttgcg
     2641 ttacttcagc gctctggaag aaggcatcaa cctgctgcca ggttttgcac cggagctgca
     2701 gggtgtctat atcgaagaac atgatggtcg taagcaagtt tggtgctatg tcatcaagcc
     2761 tcgtgatgcg caaagcaccc tgttgaaagg ggaaaaatta tgagtcaggc gaaatcgaac
     2821 aaaatgggtg tcgttcagtt aaccatactg acgatggtca acatgatggg ctccggtatc
     2881 atcatgctgc cgacaaagct tgccgaagtc gggacaatct caattatctc ctggctggtg
     2941 acagccgtag gctcaatggc actggcatgg gcattcgcaa aatgcggtat gttcagccgt
     3001 aaatcaggag gtatgggcgg ttatgccgag tatgctttcg gtaaatccgg taactttatg
     3061 gcgaactata cctacggcgt ctcgctgctg attgctaacg tcgcgattgc tatttcggcg
     3121 gttggttacg gcaccgaact gctcggcgcg agtttgtcgc cagtgcagat tggtcttgcg
     3181 accatcgggg tgctgtggat ttgtaccgtg gctaactttg gtggtgcgcg cattaccggg
     3241 caaatcagta gcattaccgt gtggggggtc attattccgg tggtaggcct gtgcattatc
     3301 ggctggttct ggtttagccc gacactttac gttgattcct ggaatccgca tcatgcaccg
     3361 ttcttcagtg cggtaggttc ttccatcgct atgacgctgt gggcttttct tggtctggag
     3421 tctgcgtgtg cgaatactga tgtagtggaa aacccggaac gtaatgtgcc aatcgcggta
     3481 ctcggcggta cgttaggtgc ggcggtgatt tatatcgtct ccaccaacgt gattgccggg
     3541 attgtgccaa atatggagct ggcaaattca acggcaccat ttggtctggc cttcgcgcag
     3601 atgttcacgc cggaagtggg taaagtcatt atggcgctga tggtgatgtc ctgctgcggt
     3661 tcgctgcttg gctggcagtt caccattgcc caggtgttta aatcttcatc tgatgaaggc
     3721 tacttcccta aaattttctc ccgtgtaacc aaagtggatg caccggtgca gggaatgttg
     3781 accattgtga ttatccagag cgggttggcg ctgatgacga ttagcccgtc gctaaacagt
     3841 cagttcaacg tgctggttaa cctggccgtg gtgaccaata tcattccgta tattctgtca
     3901 atggcggcat tagtcattat tcagaaggtc gctaatgtgc ccccctcaaa agcgaaagtt
     3961 gcaaactttg ttgcttttgt tggcgcgatg tacagctttt atgcgctgta ctcatccggg
     4021 gaagaagcca tgctatacgg ttccatcgtg accttcctcg gttggacact gtatggtctg
     4081 gtctcaccac gctttgaact gaaaaataaa cacggttaat taagcaaagc aaaaaagggc
     4141 gatcttccga ccgccctttt tcttatttga tatgtttacg cgtttttcag aacttcgcta
     4201 acaatctcaa ccgcttcttt ctcaatctgc ttgcgatgtt cttcaccaag gaagctttcg
     4261 cagtagatct tatatgcgtc ttccgtgcct gacggacgcg cggcgaacca gccgttgtca
     4321 gtcatcactt tcagaccgcc gatagaagcg ccattacccg gtgccgccgt cagacgcgcg
     4381 gtgatcgggt cacctgccag ggtgctggcg ctcaccattt ccggagacag cttagacaac
     4441 gccgcttttt gtgcggaagt cgctgctgcc tgcaaacggt tatagctcgg cgcaccaaag
     4501 cgttttgcca gttcgttata gtgttcctgc gggttcttac cggtgacagc ggtgatttcc
     4561 gccgccagca gacacataat gataccgtct ttatcggtgg accacggcgt gccgtcgaaa
     4621 cgcaggaagg aagcgcctgc actctcttcg ccgccaaagc cgaagctgcc atcgaacaaa
     4681 ccatcaacaa accatttgaa acctaccggt acttctacca gcttacggcc caaatcattg
     4741 accacacggt cgatcatcgc agatgaaacc agcgttttac cgacagcaac atctttgccc
     4801 cactgcggac gatgctggaa caggtagttg atcgccaccg ccaggtagtg gttcggattc
     4861 atcaaacctg ccggagtgac gataccgtga cggtcataat ccgggtcgtt agcaaacgcc
     4921 agatcgaact tatcacgcag tgccagcagg cccgccatcg cacactcgga ggagcagtcc
     4981 atacggatcg caccgtcttt atcgaggtgc ataaagcgga aggtttgatc gacctgatcg
     5041 ttaacgatgg tcaggttgag gttgtaatac tcgccaatgc gcttccagta ttcgataccg
     5101 gaaccaccca gcggatcaac gcccagcgtc aggcccgctt tctgaatcgc cgccatatca
     5161 acgatatcgg ccagcccttc cacaaacggc tgcaccagat cctgctcttt cacatgaccg
     5221 gatgccatcg cttcgtcgag ggagatacgc ttcacgcctt tcaggccatc agccagcagt
     5281 gcgtttgctc tgtcttccac cactttagtg acgttggtat cagccgggcc accatttggc
     5341 ggattgtact tgataccacc atcttccggc gggttatggg acggtgtaat cacgataccg
     5401 tctgccagcg ggccaccttt tttattgtga accagaatgg cattggaaat ggcaggtgtc
     5461 ggagtgaagc cattgttttc ctgcacaatg acatcaacgc cgttcgctgc cagcacttcc
     5521 agtacggaaa tgaacgcagg ctcggacagg gcgtgagtat ctttacccac atagcaaggg
     5581 ccagtgatgc cgtttttcgc acgttcttca gcaattgcct gagcgatcgc cagaatgtgc
     5641 ggctcgttaa agctgtggcg cgctgcactg ccacggtgac cggaagtacc gaatttcacc
     5701 gcgtgctccg cattccctgc ttctggtttc agtacataat attgcgccgt cagttgggcg
     5761 acgttaatca aatcactctg ttgtgcaggt tggcctgcac gattgtggat tgccattgct
     5821 ttgtcctttg tctgcaacgt tttagatagt tccgcaaacc ttctcaatca attccgccgg
     5881 gaattgcatc gactgcatga tgtgttcgat catgctgcat ttacggccgg tgttggtgtt
     5941 ggtgatcacc caatacggcg tgcctggcac atgtttcggc ttggtctgat taccattttt
     6001 cagcagcgtt tgttcatctg ccgcaaagta aacgcgtgta cgaccgtgca acgattccgt
     6061 tgcttcggca aacgcctggg cgtcaagaga atatagtgta gacaacagca gcataaagcg
     6121 attgaccgct cgcttttgct ctgcgtattc gtccgaaagc agaagttcac gcattgcgcg
     6181 aaccttgtct ttaatcgttt tgaccggctt cgcttcgacg atagcaggtg acgcaacgcg
     6241 aacctctttc gtcaccggag cagcaggctg tgatgcggcg gaaaatttca acatacgccg
     6301 taaaatgtcg gatgcgctct cgccgatatg cttagtgtgg ctggcaatat agctgtagag
     6361 ttcatcatca acttcaatcg ttttcatctt aatccagtgc agtgtcttag ctgaatacaa
     6421 gtcgccagga gtatatgaat aaatcccggc agcggatagc gtcaagcctg gcttgcagaa
     6481 aaactcggaa taaaccttat ttgttgcagc cctgtggcac aatggtcaac ataataccct
     6541 aacccgacaa cgcgaaaaaa agaactttgc catgaaattg aatatccgcg cgcaaactgc
     6601 acaaaaccag cacaataatt ctcccatcgt tcttgtccat ggtctgtttg gcagcctcga
     6661 caaccttggc gtactggctc gcgatctggt aaacgatcac aatatcatcc aggttgatat
     6721 gcgtaaccac ggtctttcac cgagagatcc ggtaatgaat tacccggcga tggcgcagga
     6781 tcttgttgat accctggatg cacagcagat cgacaaagca acatttatcg gtcactccat
     6841 gggcggtaaa gcggtaatgg ctcttactgc actagccccc gatcgcattg acaagctggt
     6901 ggcgattgat attgcgccgg tcgattatca cgttcgccgt catgatgaga ttttcgcggc
     6961 catcaacgca gtcagtgaat cagacgcaca aacacgccag caagcagcgg caataatgcg
     7021 ccagcatctt aatgaagaag gggtgattca gtttctgttg aaatcttttg ttgacgggga
     7081 gtggcgcttt aacgtgccag tattgtggga tcagtatccg catattgtgg gttgggagaa
     7141 aatcccggca tgggatcacc ctgccctgtt tatccctggc ggcaattctc cgtatgttag
     7201 cgagcagtac cgtgatgatt tactggcaca atttccacag gctcgggcgc atgtgattgc
     7261 aggcgcgggt cactgggtcc atgctgaaaa accggatgcg gtattacgcg ctatccgtcg
     7321 ctatctcaat gattaacgca actgattaaa aacccagcgt ccgcgtcgct ttccgcgcgt
     7381 ggacgttggc gtgactcatc gcgctgatgt attatggcgc gctatccatc cgggcaggcg
     7441 cctggctgat tgtttccccc gaagtcacca agatcatggc caaagaacaa acggaccgta
     7501 cgacattaga tctgttcgcg cacgagcgtc gaccgggacg accgaaaact aatccgcttt
     7561 cgcgcgatga acagctgcgt attaataaac gcaaccaact aaaacgcgac aaagtacgtg
     7621 gccttaagcg tgtcgaactg aagctgaacg cggaagctgt cgaggcactg aacgagctgg
     7681 cggaatcgcg caatatgagc cgtagcgagc tgatcgaaga gatgttaatg ctgcaactgg
     7741 cggcgctgcg tagtcagggc atcgtttaaa tttccacttt catgtagcac agtgtgcagt
     7801 cctgctcgtt tgctgattcc gcaagactgc ctgttctgct atgattgcct ttatccgtgg
     7861 gcaattttcc acccccattt caataagttt caagaggtta tttcactcat ggctatcact
     7921 ggcatctttt tcggcagcga caccggtaat accgaaaata tcgcaaaaat gattcaaaaa
     7981 cagcttggta aagacgttgc cgatgtccat gacattgcaa aaagcagcaa agaagatctg
     8041 gaagcatatg acattctgct gctgggcatc ccaacctggt attacggcga agcgcagtgt
     8101 gactgggatg acttcttccc gactctcgaa gagattgatt ttaatggtaa actggttgcg
     8161 ctgtttggtt gtggcgacca ggaagattac gcagaatact tctgcgatgc attgggcacc
     8221 atccgcgaca tcattgaacc gcgcggcgca actatcgttg gtcactggcc aaccgcgggc
     8281 taccacttcg aagcgtcaaa aggtctggct gatgatgacc actttgtcgg tctggctatc
     8341 gacgaagatc gtcagccgga actgaccgct gaacgtgtag aaaaatgggt taaacaaatt
     8401 tctgaagagc tgcatctcga cgaaattctc aatgcctgat gtgatgcggc gtagactcat
     8461 gtctacgccg tattaataga taatgccaat caaaataatt gctacaaatt tgtaactttt
     8521 gctgttgtac ctgtacaatg tcccggtgtt caagtggcct tgccgttgta aatgtaagct
     8581 gtgccacgtt tttattaaca atatttgcca gggacttgtg gttttcattt aggcgtggca
     8641 attctataat gatacgcatt atctcaagag caaattctgt cacttcttct aatgaagtga
     8701 accgcttagt aacaggacag attccgcatg actgataaca ataccgccct aaagaaagct
     8761 ggcctgaaag taacgcttcc tcgcttgaaa atcctggaag ttcttcagga gccggacaac
     8821 catcacgtca gtgcggaaga tttatacaaa cgtctgatcg atatgggtga agaaattggt
     8881 ctggctacgg tatatcgcgt actgaaccag tttgacgacg ctggtatcgt cacccgccac
     8941 aattttgaag gcggtaaatc cgtatttgaa ctaacacagc aacatcacca cgatcacctg
     9001 atctgcctcg actgcggtaa ggttatcgaa tttagtgatg attccatcga agcgcgtcag
     9061 cgtgaaatcg ccgcaaaaca tggcattcgc ctgactaacc acagtctcta tctttacggt
     9121 cactgtgccg aaggcgattg ccgcgaagat gagcacgcgc acgaaggcaa ataagccagc
     9181 ctgaacgaga aaagccaacc tgcaggttgg cttttttata caaggaaaat aagaaaagcg
     9241 agagttacag ctctcactta tttgttatta ctgcgaattt cctgccagac cttatcacaa
     9301 tcggctttca ccgcctgatc atttccggtt tgcgttgcct gaatacactg ctgataatcc
     9361 agtacgcgga cactttcctg ctcggcaaac tgctgatgtt gtttctcttt cttcagcacg
     9421 tttaacacgc tctggcaggc ttcaattttt tccggcgaac cttgcgcggt gttgatacag
     9481 gcgctgtaag cctctttcag acgactgtct tcctgcggtg cggttgattg cgcacaagct
     9541 accagccccg atgccatcat ggcgatgaga atcagttttt tcatagcaaa ttcctcaaac
     9601 gtgcggcagg cacaaaacct gccgcgtcgg gcatcagaag atggtgaatg gtgcgattac
     9661 catgaatttc acgtcgcgct catcctggaa aatgttgccg taaccaccgc cccagcttgg
     9721 gatgtcggag tggttgtcat attcggtgaa atgcagtttg aacatcgtgc ccttggcacg
     9781 accgtcctga atggtgtaaa ccgcatccag gctgtaggca gactcttcaa tagtccggtt
     9841 cttgtcgtag tacgcatccg gattgctctg ccaggtcgca ggtttagcat cccatgcgta
     9901 aacgtaggaa gcgccgatgg cgaagcctgg aagattccag tttttcaggt catacatcgc
     9961 accgaagaag accgcttttt cgccgttggc gttgaagtcg gaacggttat cccaccagat
    10021 atccaggcga ccgttcgagg aagcgtaggt tggagtcata cgttgcagga agtatccttg
    10081 ctgaccgtca gccttaaccc aggtaccttc gaggcgcaaa tccactacgt cagccgcccg
    10141 gtaaccaaag gtcaacgcct gcagccaggc ggtgccgtca taaagatcgt tgacgctgcg
    10201 atcgtcaact ttatcgcgcg taccatagaa ctggtagctg gtggttaacg ggctaccggc
    10261 gatatcaaat ttgtagctgg ctttggcaaa atattgatcg atataccctt cggcctgacc
    10321 aaacgccgct tccagtatga agttattttt gaagtcgtac ttcgccccaa gggagtgtag
    10381 ataatcaact ttggtggttt tatcgttctg ataaaactca tccatttcca gatgccacgg
    10441 tgctttgtat tcgttggtcc acatgtagga gaaactcaac gcaccagcat cgccgtaatc
    10501 aaaattcgcc ccggcctccg ccccctgata agtacccggc ataaagctcc agtgtggcgc
    10561 taacagcgtc tgaccggttg gctgaatata acctgccctc gcccaaaccg gaccgtattt
    10621 aaatttggcc gctgctttat acaggcttat accgctttta tcgccggacc agtcttcgtc
    10681 ataggcttta ttactttttg aaaacgcgat ttcgttcggg tgagagctgt cgccgttttc
    10741 cgccatttca atcgccgtaa acacggcaat atcaaggccg aacatatcag cagcataacc
    10801 agactgaaaa tcgaggttgg cgttccaggt ggagtgagaa aggttggttt tgtatttatc
    10861 gccgtcggta acatctttac ggtcgcgttc acgctgccag taatagatac cgccggttaa
    10921 ggttgaatcg tcgatgaatc cttcggcgcg agcctccgga attgccataa agcccgacat
    10981 agctgtaaca ccggcgatag ccagcgccag cgtactacgt ttgccactaa acgcacgcat
    11041 gggttaatcc tctttgacgt ataaattgct gcaccaaagg tgaacagcga aaaaaataaa
    11101 aattaaaaaa gtagcgtgga gaaaagtctc gctgactaca ggaacagact atttttcgcg
    11161 acgcgaatta tcaatctctt tctgtaacca gaatgtaaga acatgagcaa ccgcacatat
    11221 tttgatgatt tttgttacaa ggcttaagtt tgcagttgaa aggcttgcag aagaataaaa
    11281 gcg
//
LOCUS       DADSYM010000146        11239 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2415
            SAMN08039579-rid11723173.denovo.147, whole genome shotgun sequence.
ACCESSION   DADSYM010000146 DADSYM010000000
VERSION     DADSYM010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039579
            Sequence Read Archive: SRR6362533
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11239)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11239)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980162.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 03:39:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,484
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 4,347
            CDSs (with protein)               :: 4,347
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 43 of 85
            Pseudo Genes (incomplete)         :: 37 of 85
            Pseudo Genes (internal stop)      :: 19 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11239
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039579-rid11723173.denovo.147"
                     /strain="LGE2415"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8302 N 95.9628 W"
                     /collection_date="2015-08-24"
     gene            <1..650
                     /gene="gspC"
                     /locus_tag="J1Y83_003589"
     CDS             <1..650
                     /gene="gspC"
                     /locus_tag="J1Y83_003589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type II secretion system protein GspC"
                     /protein_id="HBA7631701.1"
                     /translation="QSEFSLAALWRNKNHAGVKDANPVAVNQETPKLSIVLNGIVLTS
                     NDETSFVLINEGNEQKRYSLNEALESVPGTFIRKINKTSVVFETHGHYEKVTLHPGLP
                     DIIKQPDSENQNVLADYIIATPIRDGEQIYGLRLNPRKGLNAFTTSLLQPGDIALRIN
                     NLSLTYPDEVSQALSLLLTQQSAQFTIRRNGVPRLINVSVAELTGMNGQRNEGTQ"
     gene            634..2586
                     /gene="gspD"
                     /locus_tag="J1Y83_003590"
     CDS             634..2586
                     /gene="gspD"
                     /locus_tag="J1Y83_003590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417784.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system secretin GspD"
                     /protein_id="HBA7631702.1"
                     /translation="MKGLNKITCCLLAALLMPCAGHAENEQYGANFNNADIRQFVEIV
                     GQHLGKTILIDPSVQGTISVRSNDTFSQQEYYQFFLSILDLYGYSVITLDNGFLKVVR
                     SANVKTSPGMIADSSRPGVGDELVTRIVPLENVPARDLAPLLRQMMDAGSVGNVVHYE
                     PSNVLILTGRASTINKLIEVIKRVDVIGTEKQQIIHLEYASAEDLAEILNQLISESHG
                     KSQMPALLSAKIVADKRTNSLIISGPEKARQRITSLLKSLDVEESEEGNTRVYYLKYA
                     KATNLVEVLTGVSEKLKDEKGNSRKPSSTSAMDNVAITADEQTNSLVITADQSVQEKL
                     ATVIARLDIRRAQVLVEAIIVEVQDGNGLNLGVQWANKNVGAQQFTNTGLPVFNAAQG
                     VADYKKNGGITSANPAWDMFSAYNGMAAGFFNGDWGVLLTALASNNKNDILATPSIVT
                     LDNKLASFNVGQDVPVLSGSQTTSGDNVFNTVERKTVGTKLKVTPQVNEGDAVLLEIE
                     QEVSSVDSSSNSTLGPTFNTRTIQNAVLVKTGETVVLGGLLDDFSKEQVSKVPLLGDI
                     PLVGQLFRYTSTERTKRNLMVFIRPTIIRDDDVYRSLSKEKYTRYRQEQQLRIDGKSK
                     ALVGSEDLPVLNENTFNSHAPAPSSR"
     gene            2596..4077
                     /gene="gspE"
                     /locus_tag="J1Y83_003591"
     CDS             2596..4077
                     /gene="gspE"
                     /locus_tag="J1Y83_003591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system ATPase GspE"
                     /protein_id="HBA7631703.1"
                     /translation="MRIHSPYPASWALAQRIGYLYSEGEIIYLADTPFERLLDIQRQV
                     GQCQTMTSLSQADFEARLEAVFHQNTGESQQIAQDIDQSVDLLSLSEEMPANEDLLNE
                     DSAAPVIRLINAILSEAIKETASDIHIETYEKTMSIRFRIDGVLRTILQPNKKLAALL
                     ISRIKVMARLDIAEKRIPQDGRISLRIGRRNIDVRVSTLPSIYGERAVLRLLDKNSLQ
                     LSLNNLGMTAADKQDLENLIQLPHGIILVTGPTGSGKSTTLYAILSALNTPGRNILTV
                     EDPVEYELEGIGQTQVNTRVDMSFARGLRAILRQDPDVVMVGEIRDTETAQIAVQASL
                     TGHLVLSTLHTNSASGAVTRLRDMGVESFLLSSSLAGIIAQRLVRRLCPQCRQFTPVS
                     PQQAQMFKYHQLAVTTIGTPVGCPHCHQSGYQGRMAIHEMMVVTPELRAAIHENVDEQ
                     ALERLVRQQHNALIKNGLQKVIRGDTSWDEVMRVASATLENEA"
     gene            4074..5270
                     /gene="gspF"
                     /locus_tag="J1Y83_003592"
     CDS             4074..5270
                     /gene="gspF"
                     /locus_tag="J1Y83_003592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system inner membrane protein
                     GspF"
                     /protein_id="HBA7631704.1"
                     /translation="MNYRYRAMTQDGQKLQGIIDANDERQARLRLREEGLFLLDIRPQ
                     KSSGVKTRRPRISHSELTLFTRQLATLSAAALPLEESLAVIGQQSSNNRLADVLNQVR
                     SAILEGHPLSDALQHFPTLFDSLYRTLVKAGEKSGLLAPVLEKLADYNENRQKIRSKL
                     IQSLIYPCMLTTVAIVVVIILLTAVVPKITEQFVHMKQQLPLSTRILLGLSDTLQRTG
                     PTLLATVFIVAVGFWLWLKRGNNRHRFHAMLLRVALIGPLICAINSARYLRTLSILQS
                     SGVPLLDGMNLSTESLNNLEIRQRLANAAENVRQGNSIHLSLEQTAIFPPMMLYMVAS
                     GEKSGQLGTLMVRAADNQETLQQNRIALTLSIFEPALIITMALIVLFIVVSVLQPLLQ
                     LNSMIN"
     gene            5280..5717
                     /gene="gspG"
                     /locus_tag="J1Y83_003593"
     CDS             5280..5717
                     /gene="gspG"
                     /locus_tag="J1Y83_003593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system major pseudopilin GspG"
                     /protein_id="HBA7631705.1"
                     /translation="MRATDKQRGFTLLEIMVVIVIIGVLASLVVPNLMGNKEKADKQK
                     AVSDIVALENALDMYKLDNHRYPTTNQGLESLVEAPTLPPLAANYNKEGYIKRLPADP
                     WGNDYVLVNPGEHGAYDLLSAGPDGEMGTEDDITNWGLSKKKK"
     gene            5725..6234
                     /gene="gspH"
                     /locus_tag="J1Y83_003594"
     CDS             5725..6234
                     /gene="gspH"
                     /locus_tag="J1Y83_003594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspH"
                     /protein_id="HBA7631706.1"
                     /translation="MNQQRGFTLLEMMLVLALVAITASVVLFTYGREDAASTRARETA
                     ARFTAALELAIDRATLSGQPVGIHFSDSAWRIMVPGKTPSAWRWVPLQEDAADESKND
                     WGEELSIQLQPFKSDDSNQPQVVILADGQITPFSLLMANAGTGEPLLTLVSSGSWPLD
                     QTLARDTRP"
     gene            6231..6608
                     /gene="gspI"
                     /locus_tag="J1Y83_003595"
     CDS             6231..6608
                     /gene="gspI"
                     /locus_tag="J1Y83_003595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417789.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspI"
                     /protein_id="HBA7631707.1"
                     /translation="MNKQSGMTLLEVLLAMSIFTAVALTLMSSMQGQRTAIERMRNET
                     LALWIADNQLQSQDTFDEENTSSSGKELINGEEWNWRSDIHSSKDGTLLEHTITVTLP
                     SGQTTSLTRYQSINNKSGQAQDD"
     gene            6601..7188
                     /gene="gspJ"
                     /locus_tag="J1Y83_003596"
     CDS             6601..7188
                     /gene="gspJ"
                     /locus_tag="J1Y83_003596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspJ"
                     /protein_id="HBA7631708.1"
                     /translation="MINRQQGFTLLEVMAALAIFSMLSVLAFMIFSQVSELHQRSQKE
                     IQQFNQLQRTITILDNDLLQLVARRNRSTDKIMVLGEEAIFTTQSRDPLAPLSEAQTL
                     LTVHWYLRNHTLYRAVRTSVDGRKDQPAQAMLEHVESFLLESNSGESQELPLSVTLHL
                     KTQQYGALQRRFALPEQLAREESPAQTQAGNNNHE"
     gene            7181..8164
                     /gene="gspK"
                     /locus_tag="J1Y83_003597"
     CDS             7181..8164
                     /gene="gspK"
                     /locus_tag="J1Y83_003597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspK"
                     /protein_id="HBA7631709.1"
                     /translation="MNNEQRGVALLIVLMLLALMAALAADMTISFHGQLHRTRQVNHH
                     LQRQYDIELAEKLALASLTLDVKDNDRQTTLQQYWAQPQQLQLENGNTVKWQLRDAQH
                     CFNLNALAKISDDPLASPDFPAQVFSALLINAGIDRGNTDEIVQSIADYIDVDDSPRF
                     HGAEDNFYQSQTPPRHSANQMLFLTGELRQIKGITENIYQRLIPYVCVLPTSELSINL
                     NMLTENDIPLFRALFLNNITDADARVLLQKRPREGWLTTDAFLYWAQQDFSGVKPLVA
                     QVKGHLFPYSRYFTLSTESISDEQSQGWQSHIFFNRKQQSAQIYRRTLQLY"
     gene            8179..9342
                     /gene="gspL"
                     /locus_tag="J1Y83_003598"
     CDS             8179..9342
                     /gene="gspL"
                     /locus_tag="J1Y83_003598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspL"
                     /protein_id="HBA7631710.1"
                     /translation="MPESLMVIRSFSTLRKHWEWMTFSADSVSSVHTLTDDLPLESLA
                     DQPGAGNVHLLIPPEGLLYRSLTLPNAKYKLTAQTLQWLAEETLPDASQNWHWTVVDK
                     QNESVEVIGIQSEKLSRYLERLHTAGLNVTRVLPDGCYLPWEVDSWTLVNQQTSWLIR
                     SAAHAFNELDEHWLQHLANQFPPENMRCYGVAPHGVAAANPLIQHPEIPSLSLYSADI
                     AFQRYDMLHGVFRKQKTVSKSGKWLARLAVSCLVLAILSFVGSRGIALWQTLKIEDQL
                     QQQQQETWQRYFPQIKHTHNFRFYFKQQLAQQYPEAVPLLYHLQTLLLEHPELQLMEA
                     NYSQRQKSLTLKMSAKSEANIDRFCELTQSWLPMEKTEKDPVSGVWTVRNSGK"
     gene            9339..9800
                     /locus_tag="J1Y83_003599"
     CDS             9339..9800
                     /locus_tag="J1Y83_003599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417793.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein M"
                     /protein_id="HBA7631711.1"
                     /translation="MIKSWWAEKSTSEKQIVAALAVLSLGVFCWLAVIKPIDTYIEEH
                     QSHAQKIKKDIKWMQDQASTHGLLGHPALTQPIKNILLEEAKRENLTITLENGPDNTL
                     TINPVTAPLENVSRWLTTAQITYGIVIEDLQFTLAGNEEITLKHLSFREQQ"
     gene            9800..10477
                     /locus_tag="J1Y83_003600"
     CDS             9800..10477
                     /locus_tag="J1Y83_003600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HBA7631712.1"
                     /translation="MTMLLPLFILVGFIAGYFVNAMAYHLSPLEDKTALTFRQVLVHF
                     WQKKYAWHDTVPLILCVAAAIACALAPFTPIVTGALFLYFCFALTLSVIDFRTQLLPD
                     KLTLPLLWLGLVFNAQSGLIDLHDAVYGAVAGYGVLWCVYWGVWLVCHKEGLGYGDFK
                     LLAAAGAWCGWQTLPMILLIASLGGIGYAIVSQLLQRRTITTIAFGPWLAFGSMINLG
                     YLAWISY"
     gene            complement(10506..10982)
                     /gene="bfr"
                     /locus_tag="J1Y83_003601"
     CDS             complement(10506..10982)
                     /gene="bfr"
                     /locus_tag="J1Y83_003601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin"
                     /protein_id="HBA7631713.1"
                     /translation="MKGDTKVINYLNKLLGNELVAINQYFLHARMFKNWGLKRLNDVE
                     YHESIDEMKHADRYIERILFLEGLPNLQDLGKLNIGEDVEEMLRSDLALELDGAKNLR
                     EAIGYADSVHDYVSRDMMIEILRDEEGHIDWLETELDLIQKMGLQNYLQAQIREEG"
     gene            complement(11054..11224)
                     /gene="bfd"
                     /locus_tag="J1Y83_003602"
     CDS             complement(11054..11224)
                     /gene="bfd"
                     /locus_tag="J1Y83_003602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001450353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin-associated ferredoxin"
                     /protein_id="HBA7631714.1"
                     /translation="MSDKKIRQAVRQFSPHSFQQLKKFIPVGNQCGKCVRAAREVMED
                     ELMQLPEFKESA"
BASE COUNT         2928 a         2886 c         2687 g         2738 t
ORIGIN      
        1 ttcagtctga attcagcctt gccgctttat ggcgcaacaa aaatcatgct ggggtaaaag
       61 atgcaaatcc cgtggcagtt aatcaggaaa cacctaagct ctctatagtg cttaatggca
      121 ttgtactcac atcgaatgat gagacttctt ttgtcttaat taatgagggg aacgaacaaa
      181 agcgatactc cctcaacgaa gcccttgagt cagtgccggg aacgtttatt agaaaaataa
      241 ataaaaccag cgttgtgttt gaaactcacg ggcattacga aaaagtaact ctccatccag
      301 ggctacctga catcatcaag cagcctgatt cggaaaatca aaatgtactc gccgattata
      361 ttatcgccac acctatacgt gatggagaac agatatacgg tcttcgattg aatcctcgta
      421 agggtctcaa tgcatttacc accagcttgc tgcaacctgg ggatatcgcc ctcaggatca
      481 ataatctttc gctgacctac cctgatgagg tttcacaggc attaagttta ctgctaactc
      541 aacaaagtgc tcagtttaca attcgtcgca acggcgtacc ccgcttgata aatgtttccg
      601 tcgcggaact tacaggaatg aatggacagc gcaatgaagg gactcaataa aatcacctgc
      661 tgcttgctgg cagcactact catgccttgt gcaggacacg ctgagaacga acaatacggc
      721 gctaacttca ataacgccga tatccgccag ttcgtggaaa tagtgggtca gcatcttggc
      781 aaaacgatcc tgatcgaccc ttcggtacag ggaaccattt ccgtacgcag taatgatacg
      841 tttagccaac aggagtacta ccagttcttt ttaagtattc ttgatcttta cggttattcc
      901 gtgatcacac tggacaatgg ttttctgaaa gtggtgcgct cagctaatgt aaaaacatcg
      961 ccagggatga ttgctgacag ttctcgtcca ggcgtaggtg atgagttggt cacccgaatc
     1021 gtaccgcttg agaacgttcc tgctcgtgac ctggctcccc tgctccgcca gatgatggat
     1081 gcgggtagcg tcggtaatgt tgtgcattat gaaccctcca acgttcttat tctgaccggt
     1141 cgtgcctcca ccattaataa actgattgaa gtcataaagc gcgttgatgt catcggcaca
     1201 gagaagcagc aaattattca tctggaatat gcatcagcgg aagatctcgc cgagattctt
     1261 aatcaattaa tcagcgaaag ccacggtaaa agccagatgc ctgccctcct ttccgcgaag
     1321 attgtggcgg ataagcgaac caactctctt atcatcagcg ggccggaaaa agcacgccag
     1381 cgcataactt cattactgaa aagccttgat gtcgaagaga gcgaggaagg aaatacccgg
     1441 gtctattacc tgaaatatgc taaagccacg aatctggtgg aagtgctaac cggtgtttcc
     1501 gaaaagctga aagatgaaaa agggaattcg cgtaagcctt cttctacttc tgcgatggat
     1561 aatgtggcca ttaccgccga tgaacagacc aactcgttgg tcattaccgc tgaccagtcc
     1621 gtccaggaga aactcgccac ggtaattgcg cgtctggata ttcgccgtgc acaggtgctg
     1681 gttgaggcaa ttatcgtcga agttcaggat ggaaatggac tcaacctcgg cgtgcaatgg
     1741 gcgaataaaa atgttggcgc acagcaattt accaatacgg gattaccggt ttttaacgct
     1801 gcgcaaggtg tagctgatta taaaaagaat ggcgggatca ccagcgcgaa tcctgcctgg
     1861 gatatgttta gcgcctacaa tggcatggct gcaggcttct tcaatggcga ctggggcgta
     1921 ttgttgactg cgctggccag taacaataaa aatgacatcc tcgccacccc gagcatcgta
     1981 acgctggata ataaactcgc gtccttcaac gttggtcagg atgtgccggt gctatccggc
     2041 tctcagacca cttctgggga taacgtcttt aatactgtcg aacgcaaaac ggtgggcaca
     2101 aaactcaaag tcactccgca ggtcaatgaa ggcgacgcgg tactgctcga aatagagcag
     2161 gaggtttcca gcgttgactc ttcgtctaat tcgacgctcg gcccgacatt taacacccgt
     2221 actattcaaa acgccgtgct ggttaaaacc ggtgaaacgg tggtcctggg cggattgctg
     2281 gatgattttt ctaaagagca agtgtcaaag gttcctctgc ttggcgatat tcctttagtg
     2341 ggacaactct tccgctatac ctcgaccgag cgcactaaac gcaacctgat ggtatttatc
     2401 cgcccgacga ttatccgtga cgatgatgtt tatcgctcac tgtcaaaaga gaaatacacc
     2461 cgctaccgtc aggagcaaca gctgcgaatc gacgggaaat caaaagcgct ggttggctcg
     2521 gaagatttgc cggtgctgaa tgagaacacg ttcaacagtc acgcccctgc gccatcgtca
     2581 cggtgaggca ttcttatgag aattcactca ccgtaccccg ccagttgggc gctggcacaa
     2641 cgaattggtt atctctattc agagggcgag attatttatc tcgccgatac gccattcgaa
     2701 cggttactcg atattcaacg tcaagttggc cagtgccaga ccatgaccag cttgtcacag
     2761 gctgattttg aagcccgact ggaagcggtg ttccatcaga ataccggtga gtcgcaacag
     2821 attgcgcagg atatcgatca atccgtcgat cttctctcgc tttcggaaga gatgcccgca
     2881 aatgaagatc tcctgaatga agattcagcg gcaccggtta tccgcttgat caatgctatt
     2941 ttgagtgagg ccatcaaaga aaccgcctcc gatatccaca ttgaaaccta tgaaaaaaca
     3001 atgtcgatcc gttttcgcat cgacggcgtt ttgcggacaa ttttacagcc caacaaaaaa
     3061 ctggcggcac tgcttatctc ccgaattaag gtcatggctc gtcttgatat cgccgaaaaa
     3121 cgtattccac aggatggaag aatcagtttg cgtatcgggc gacgtaacat agatgtccgc
     3181 gtatccacac tgccatccat ctatggtgaa cgcgctgtgc tccgcctgct ggataaaaac
     3241 agcctccagc tttcattgaa caacctgggg atgacggcag cggataaaca ggatttagaa
     3301 aatctcattc agcttccgca cggtattatc ctggtaacag ggccgacagg ctccggtaaa
     3361 agcaccacgc tctacgccat tctttcggcg cttaataccc ctggccgtaa tattctgacg
     3421 gtagaagatc ccgtggaata tgaactggaa ggcataggac aaacgcaggt gaatacccgc
     3481 gtggatatgt ctttcgctcg cggcctgcgc gccatacttc gccaggaccc ggatgtcgtc
     3541 atggtggggg agattcgtga tacagaaacc gcgcaaattg cggttcaggc ctcgctcaca
     3601 ggccatctgg tactctcaac actccacact aacagtgcat caggcgcagt cacccggcta
     3661 cgcgacatgg gcgtcgaatc attcctgctt tcgtcttcac tggcagggat tatcgcgcaa
     3721 cgtctggttc gtcgtctgtg tccgcaatgc cgacaattca cgcccgtatc gccccagcaa
     3781 gcgcagatgt ttaaatatca tcagctcgcg gtgacgacaa ttggcactcc cgtaggctgc
     3841 cctcattgcc atcaatccgg ctaccagggg cgcatggcga tccacgaaat gatggtggtg
     3901 acgccggaat tgcgggccgc tattcatgaa aatgtggatg aacaagcact ggagcgactg
     3961 gtccggcaac aacacaatgc cttaatcaaa aatggcctgc aaaaagtgat acgcggtgac
     4021 acctcctggg atgaggttat gcgcgtcgcc agtgccacgc tggagaacga agcatgaatt
     4081 atcgctatcg cgccatgacc caggatggtc aaaaattgca ggggatcatt gatgctaacg
     4141 atgaacgtca ggcacgactg cggctgcgtg aagaagggct tttcctgttg gatattcgcc
     4201 cccaaaaaag ttcgggagta aaaacacgtc gcccgaggat cagccatagt gaactaacgc
     4261 ttttcacccg gcagttggca accttaagcg cagcggcatt acccctggaa gagagccttg
     4321 ccgtaattgg tcaacaaagc agtaataatc gactggctga cgtgttaaat caggtacgca
     4381 gcgccatcct tgaagggcat cccctttccg atgcgttaca acattttccc acgcttttcg
     4441 attcgctcta ccgtaccctg gtaaaagcgg gcgaaaagag cggtctgctg gccccggtgt
     4501 tggaaaagct ggctgattac aatgaaaacc ggcagaaaat ccgcagcaag ctcattcagt
     4561 cactgatcta cccctgcatg ctcactacgg tggcgattgt ggtcgtgatt attctcctca
     4621 ctgctgtcgt gcccaaaatt accgaacagt tcgtgcatat gaagcagcaa ctgccactga
     4681 gtacacgcat tcttttaggt ctgagcgaca cgttgcaacg taccggcccg acattattag
     4741 cgacagtatt tattgtcgct gtgggtttct ggctctggtt aaaacgcggc aataaccgcc
     4801 accgttttca tgccatgtta ctgcgcgttg cgctcatcgg cccgctgatt tgcgccatta
     4861 acagcgcacg ctatctccgc actttaagta ttttgcaatc cagcggcgtc cctctgctgg
     4921 atgggatgaa tttgtccacc gaaagcctca acaacctcga aattcgccag cgcctggcaa
     4981 atgcggcaga aaacgttcgc cagggtaaca gcattcatct ttcgctggaa caaaccgcaa
     5041 ttttcccgcc gatgatgctc tacatggtgg cctctggcga aaaaagcggg caactcggca
     5101 cgttaatggt cagagccgca gataaccagg agacactcca acaaaatcgg atcgccttaa
     5161 cgctctccat cttcgagcca gcactcatta ttacgatggc actgatcgtc ctgtttattg
     5221 tcgtgtcggt actccaacct cttcttcaac ttaactcaat gattaattaa ggaaatcata
     5281 tgcgcgcaac ggataagcaa cgcggtttta cattactgga aattatggtg gtaattgtca
     5341 tcattggcgt actcgccagc ctggtggttc ccaacctgat gggcaataaa gaaaaggcgg
     5401 ataaacaaaa agccgtcagc gatattgtgg cgctggaaaa cgccctggat atgtacaagc
     5461 tcgacaatca tcgctatcca accacaaatc aggggcttga atccttagta gaagcgccga
     5521 cactgccacc gctggccgca aactataaca aggaaggtta tatcaagcgt ctgcctgccg
     5581 atccctgggg caatgattat gtcctcgtta atcctggtga acatggtgca tacgatctgc
     5641 tttcagcagg acccgatggt gaaatgggaa ccgaggacga catcaccaac tggggtttga
     5701 gcaagaagaa aaagtaagtc agcgatgaat cagcaacgcg ggtttaccct gctggagatg
     5761 atgctggtgc tggcgctggt cgctatcacg gcaagtgtgg tgctcttcac atatggtcgg
     5821 gaggatgccg ccagcactcg tgcgcgcgaa acagcggcgc ggtttaccgc tgcacttgaa
     5881 ttagccatcg accgcgccac gctcagtggt cagcctgtcg gtatccattt ctctgattca
     5941 gcatggcgca ttatggtacc gggcaaaaca ccatcagcct ggcgctgggt tccattacaa
     6001 gaagatgccg cagacgagag caaaaatgac tggggtgaag aactctcaat tcaacttcag
     6061 ccatttaaat ctgatgacag caaccaaccg caggtggtga ttctggccga cggacaaatc
     6121 actcccttct ctttgttgat ggctaatgcc gggaccggtg agccgctcct gacgttagtc
     6181 agttcaggct catggcccct ggatcaaacg ctcgccagag atacccgccc atgaacaaac
     6241 aatcagggat gacactgctt gaagtcttac tggcgatgag tattttcacc gctgttgcgc
     6301 tgactttaat gagcagtatg caagggcaac gaactgccat cgagcggatg cgtaacgaga
     6361 cgctggcatt gtggatagcg gataaccagc ttcagtcaca agatacattc gacgaggaaa
     6421 acacgtccag ttctggcaaa gagttaataa atggcgaaga gtggaactgg cgcagcgata
     6481 tccactcaag caaagacggc acgcttctgg aacacaccat aacggtgaca ttacccagcg
     6541 gtcagacaac ctcgcttacg cgctatcaaa gcatcaataa taaatccggg caggcacaag
     6601 atgattaatc gccagcaggg tttcacattg ctggaagtca tggcggcgct ggcgattttc
     6661 tcaatgctta gcgtgctggc gtttatgatt ttttctcaag tttccgagtt gcatcaacgt
     6721 tcacagaaag aaatccagca gttcaatcag ctacagcgca ccatcaccat tcttgataac
     6781 gatctcctgc aactggtcgc ccggcggaat cggagtacgg acaaaatcat ggtgttgggt
     6841 gaagaagcga tttttaccac gcaaagtcgc gatccgctgg cccctctcag tgaagcacaa
     6901 accttactca ctgttcactg gtatctgcga aatcatacgc tctaccgtgc tgttcgtact
     6961 tctgtggatg gcaggaagga tcaacccgcc caggcgatgc tggaacatgt cgagagcttt
     7021 cttctggaaa gtaacagcgg agaaagccag gagcttcctc tctcagtcac gttacatctg
     7081 aagactcagc aatatggggc gttacagcgc cgttttgcat tacctgagca gttagcgaga
     7141 gaagagtcgc cagctcagac gcaggccggg aataataacc atgaataatg aacaacgcgg
     7201 cgtagcactg ctcattgtac tcatgttact ggcgctcatg gctgctctgg ctgcagacat
     7261 gacgatcagt tttcacggcc agcttcatcg aacccgccag gtaaaccatc atttgcaacg
     7321 gcaatatgat attgagctgg cggagaaact tgcgcttgcc agcctcaccc tggacgttaa
     7381 agataacgac agacaaacca cgttgcagca gtactgggca caaccgcaac agttgcagct
     7441 ggaaaatggc aatacagtga agtggcagtt acgcgatgcc cagcactgtt ttaatctcaa
     7501 tgcgttagca aaaatctctg acgacccgct ggcttcccct gatttccccg cccaggtatt
     7561 cagtgcgctg ctgattaacg ccggtatcga tcgcggaaac accgatgaga tcgtgcaatc
     7621 aatcgctgac tatattgatg tcgatgactc accacgcttt cacggcgcag aagataactt
     7681 ttaccaaagc caaacgccgc ctcgccatag cgccaatcag atgctttttc tgaccggtga
     7741 attgcgtcag ataaaaggca taaccgaaaa catctatcag cggcttatcc cgtatgtctg
     7801 cgttctcccg acctcggaac ttagcatcaa tcttaatatg ctaacggaga acgatattcc
     7861 gctcttcaga gcgttattcc tcaataacat cacggatgct gacgctcggg tcttattaca
     7921 aaaaaggccc agggaaggct ggctaaccac agatgcattt ctttactggg cgcagcagga
     7981 tttctccggc gtgaagcccc tggttgctca ggtgaaaggg catctctttc cctatagccg
     8041 ctattttacg ctgagtacgg aaagtatcag cgacgaacaa tcccagggat ggcaaagcca
     8101 tatttttttc aaccgtaaac agcagagcgc ccaaatctat cggcgtacgc tgcaactcta
     8161 ctaatcataa ggatcgtgat gcctgaatcg ttgatggtaa tacgttcttt ctcgacgctg
     8221 cgtaagcatt gggaatggat gacgttctcc gcagatagcg tatcttcggt acatacgctc
     8281 acagacgatc ttccgctgga gtcgctggcc gatcagcccg gtgccggaaa tgttcacctt
     8341 ttgatcccac ctgagggtct tttatatcgt tccctcacgc tgcccaacgc caaatacaaa
     8401 ctcaccgcgc aaacattaca gtggttggcg gaggagacat tacccgacgc cagccaaaat
     8461 tggcactgga cggttgtcga taaacaaaac gaaagtgtcg aggtgatagg cattcagtcg
     8521 gagaaactca gtcgttatct tgagcgttta catactgccg gactcaacgt gacgcgggtt
     8581 ttgccagatg gctgctatct tccctgggaa gtagatagct ggactctggt taaccagcaa
     8641 acaagctggc ttattcgcag tgccgcacat gccttcaacg aactggatga acactggtta
     8701 cagcatcttg ctaaccagtt tccgccagag aatatgcgtt gttatggtgt agcgcctcac
     8761 ggcgttgcag cggccaatcc acttatccag catcctgaaa taccatcact gagtttgtat
     8821 tctgccgata ttgcattcca gcgctatgac atgctgcatg gtgtttttcg taaacagaaa
     8881 actgtaagca aatcgggaaa atggctcgcc cgcctggcag taagctgtct cgttctcgcc
     8941 attctttctt ttgttggcag ccggggcatc gcgctctggc aaacgcttaa gattgaagat
     9001 caacttcagc aacaacagca agaaacctgg caacgttatt tcccgcagat caaacacacc
     9061 cacaactttc gtttttactt taagcagcaa ctcgctcagc aatatcctga agctgtaccg
     9121 ttgctctatc atttacaaac acttctgctt gaacaccctg aactgcagtt aatggaggcg
     9181 aactatagcc agaggcaaaa gtcgctgacc ttaaaaatga gtgctaagag tgaagccaat
     9241 atcgatcgtt tctgtgagtt aacacagtcc tggcttccga tggaaaaaac agaaaaagat
     9301 ccggtcagtg gtgtatggac agtaaggaac tcagggaaat gattaaatca tggtgggcag
     9361 aaaaatccac atcggaaaaa cagatcgttg cggcattggc ggttctctcg ctcggcgttt
     9421 tttgttggct tgcggtgata aaaccgatag atacctacat tgaagagcat cagtcccacg
     9481 cgcaaaaaat taagaaggac atcaaatgga tgcaggatca agcgagcacc catggtttac
     9541 tcggtcatcc agcgcttacg cagcccatca aaaatatcct tctcgaagaa gcaaaacgtg
     9601 agaatttgac tatcacgctg gaaaacggtc cggacaatac tctgacaatt aaccctgtca
     9661 cagctcccct cgaaaacgtc tcccgctggc tcactacggc acagataaca tacggtattg
     9721 ttattgaaga ccttcagttt actcttgccg gaaacgaaga gatcaccctc aagcatttgt
     9781 ccttcaggga gcaacaataa tgacaatgct actgccgctc ttcattctgg ttggttttat
     9841 tgcaggttat tttgttaatg ccatggccta tcacctctcg cctttggagg ataaaacggc
     9901 gttaacgttt cgccaggtat tggttcattt ctggcaaaaa aaatatgcct ggcatgatac
     9961 agtgcccctg atactttgcg ttgctgctgc tatcgcctgc gccctggcac ctttcacgcc
    10021 cattgtgact ggcgcactct ttctctattt ctgtttcgca ctcacgctca gtgttattga
    10081 ttttcgcact cagctcctgc ccgacaaact caccttaccg ctgctctggc ttggcttggt
    10141 atttaatgcg cagtctggat taattgattt acatgatgcg gtttatggcg cggtcgctgg
    10201 ctatggggtg ctgtggtgtg tttactgggg cgtctggtta gtttgtcaca aagagggatt
    10261 gggctacggt gatttcaagc tactggccgc tgcaggcgcg tggtgtggct ggcaaacgtt
    10321 gccaatgata ctgctgattg cctcgctggg tggcattggt tacgccatcg tttcacaact
    10381 tctgcaacgc cggactataa ccactatcgc attcggcccg tggcttgcgt tcggcagcat
    10441 gataaacctg ggatatctgg cctggatctc ttattaaccg ggagggttcg ccctcccgac
    10501 acggctcaac cttcttcgcg aatctgtgct tgcagataat tttgcagtcc catcttctga
    10561 atcagatcaa gttccgtttc cagccagtcg atatggcctt cttcatcgcg caaaatttct
    10621 atcatcatat cgcggctgac gtaatcatga acgctatcgg cataaccaat tgcctcacgc
    10681 aaattcttcg cgccatccag ctcaagtgcc agatcagaac gcagcatttc ctcaacatct
    10741 tcaccaatgt tcagtttgcc caggtcctgt aagtttggaa gaccttccag aaaaagaatg
    10801 cgctcaatat aacgatcggc gtgtttcatc tcatcaatgg attcatgata ctccacatca
    10861 ttgagacgtt tgagacccca gtttttaaac atccgggcat gaagaaagta ctgattgatt
    10921 gcgacaagct catttcccaa cagtttgttg agataattta taactttagt atcacctttc
    10981 attttttgac tccttcgctt ccactctttg aagcgtagaa cggcttacga gtaagtcaaa
    11041 aaacacactc cgtttatgcg gactccttaa actccggcag ttgcattaat tcatcctcca
    11101 tcacttcacg cgcggcccgg acacatttac cgcactgatt gccgaccgga atgaattttt
    11161 ttaattgctg gaacgagtgg ggggaaaact ggcgcacagc ctgacgaatt tttttatcgc
    11221 tgataccatt acaaagaca
//
LOCUS       DADSYM010000147        11191 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2415
            SAMN08039579-rid11723173.denovo.148, whole genome shotgun sequence.
ACCESSION   DADSYM010000147 DADSYM010000000
VERSION     DADSYM010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039579
            Sequence Read Archive: SRR6362533
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11191)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11191)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980162.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 03:39:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,484
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 4,347
            CDSs (with protein)               :: 4,347
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 43 of 85
            Pseudo Genes (incomplete)         :: 37 of 85
            Pseudo Genes (internal stop)      :: 19 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11191
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039579-rid11723173.denovo.148"
                     /strain="LGE2415"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8302 N 95.9628 W"
                     /collection_date="2015-08-24"
     gene            complement(59..295)
                     /locus_tag="J1Y83_003603"
     CDS             complement(59..295)
                     /locus_tag="J1Y83_003603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313355.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1435 domain-containing protein"
                     /protein_id="HBA7631715.1"
                     /translation="MLQRTLGSGWGVLLPGLLIAGLMYADLSPDQWRIVILMGLVLTP
                     VMLYHKQLRHYVLLPSCLALIAGIMLMIMNLNQG"
     gene            436..1224
                     /gene="fhuF"
                     /locus_tag="J1Y83_003604"
     CDS             436..1224
                     /gene="fhuF"
                     /locus_tag="J1Y83_003604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore-iron reductase FhuF"
                     /protein_id="HBA7631716.1"
                     /translation="MAYRSAPLYEDVIWRTHLQPQDAGLAQAVRATIAEHREHLLEFI
                     RLDEPAPLNAMTLAQWSSPNALSSLLAVYSDHIYRNQPLMIRENKPLISLWAQWYIGL
                     MVPPLMLALLTQEKALDVSPEHVHVEFHETGRAACFWVDVCEDKNATLHSPQQRMETL
                     ISQALVPVVQALEATGEINGKLIWSNTGYLINWYLTEMKQLLGEATVESLRYALFFEK
                     TLTTGEDNPLWRTVVLRDGLLVRRTCCQRYRLPDVQQCGDCTLK"
     gene            complement(1262..1939)
                     /gene="bglJ"
                     /locus_tag="J1Y83_003605"
     CDS             complement(1262..1939)
                     /gene="bglJ"
                     /locus_tag="J1Y83_003605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418786.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator BglJ"
                     /protein_id="HBA7631717.1"
                     /translation="MEHSRIKKRNVALIEKCVMSSIGIESLFRKFAGNPYKLHTYTSQ
                     ESFQDAMSRISFAAVIFSFSAMRSARREGLSCLTELAIKFPRTRRLVIADDDIEARLI
                     GSLSPSPLDGVLSKASTLEIFHQELFLSLNGVRQATDRLNNQWYINQSRTLSPTEREI
                     LRFMSRGYSMTQIAEQLKRNIKTIRAHKFNVMSKLGVSSDAGLLEAADILLCMRHCET
                     SNVLHPY"
     gene            complement(1897..2622)
                     /locus_tag="J1Y83_003606"
     CDS             complement(1897..2622)
                     /locus_tag="J1Y83_003606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBA7631718.1"
                     /translation="MLPGCCKNGIVISKIPVMQAGLKEVMRTHFPEYEIISSASAEDL
                     TLLQLRRSGLVIADLAGESEDPRSVCEHYYSLISQYREIHWVFMVSRSWYSQAVELLM
                     CPTATLLSDVEPIENLVKTVRSGNTHAERISAMLTSPAMTETHDFSYRSVILTLSERK
                     VLRLLGKGWGINQIASLLKKSNKTISAQKNSAMRRLAIHSNAEMYAWINSAQGARELN
                     LPSVYGDAAEWNTAELRREMSHS"
     gene            3242..4012
                     /locus_tag="J1Y83_003607"
     CDS             3242..4012
                     /locus_tag="J1Y83_003607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418784.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter ThrE family protein"
                     /protein_id="HBA7631719.1"
                     /translation="MQTEQQRAVTRLCIQCGLFLLQHGAESALVDELSSRLGRALGMD
                     SVESSISSNAIVLTTIKDGQCLTSTRKNHDRGINMHVVTEVQHIVILAEHHLLDYKGV
                     EKRFSQIQPLRYPRWLVALMVGLSCACFCKLNNGGWDGAVITFFASTAAMYIRQLLAQ
                     RHLHPQINFCLTAFAATTISGLLLQLPTFSNTPTIAMAASVLLLVPGFPLINAVADMF
                     KGHINTGLARWAIASLLTLATCVGVVMALTIWGLRGWV"
     gene            4003..4476
                     /locus_tag="J1Y83_003608"
     CDS             4003..4476
                     /locus_tag="J1Y83_003608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418783.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter"
                     /protein_id="HBA7631720.1"
                     /translation="MGVIEFLLALAQDMILAAIPAVGFAMVFNVPVRALRWCALLGAI
                     GHGSRMILMTSGLNIEWSTFMASMLVGTIGIQWSRWYLAHPKVFTVAAVIPMFPGISA
                     YTAMISAVKISQLGYSEPLMITLLTNFLTASSIVGALSIGLSIPGLWLYRKRPRV"
     gene            4584..5123
                     /gene="dnaT"
                     /locus_tag="J1Y83_003609"
     CDS             4584..5123
                     /gene="dnaT"
                     /locus_tag="J1Y83_003609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaT"
                     /protein_id="HBA7631721.1"
                     /translation="MSSRVLTPDVVGIDALVHDHQTVLAKAEGGVVAVFDNNAPAFYA
                     VTPARLAELLALEEKLARPGSDVALDDQLYQEPQTAPVAVPMGKFAMYPDWQPDADFI
                     RQAALWGVALREPVTAEELASFIAYWQAEGKVFHHVQWQQKLARSLQIGRASNGGLPK
                     RDVNTVSEPDSQIPPGFRG"
     gene            5126..5863
                     /gene="dnaC"
                     /locus_tag="J1Y83_003610"
     CDS             5126..5863
                     /gene="dnaC"
                     /locus_tag="J1Y83_003610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001731874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication protein DnaC"
                     /protein_id="HBA7631722.1"
                     /translation="MKNVGDLMQRLQKMMPAHIKPAFKTGEELLAWQKEQGAIRSAAL
                     ERENRAMKMQRTFNRSGIRPLHQNCSFENYRVECEGQMNALSKARQYVEEFDGNIASF
                     IFSGKPGTGKNHLAAAICNELLLRGKSVLIITVADIMSAMKDTFRNSGTSEEQLLNDL
                     SNVDLLVIDEIGVQTESKYEKVIINQIVDRRSSSKRPTGMLTNSNMEEMTKLLGERVM
                     DRMRLGNSLWVIFNWDSYRSRVTGKEY"
     gene            5912..6406
                     /locus_tag="J1Y83_003611"
     CDS             5912..6406
                     /locus_tag="J1Y83_003611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2501 domain-containing protein"
                     /protein_id="HBA7631723.1"
                     /translation="MMKTIKHLLCCAIAASALISTGVHAASWKDALSSAASELGNQNN
                     TTQEGGWSLASLTNLLSSGNQALSADNMNNAAGILQYCAKQKLASVTDAENIKNQVLE
                     KLGLNSEEQKEDTNYLDGIQGLLKTKDGQQLNLDNIGTTPLAEKVKTKACDLVLKQGL
                     NFIS"
     gene            6660..8951
                     /gene="opgB"
                     /locus_tag="J1Y83_003612"
     CDS             6660..8951
                     /gene="opgB"
                     /locus_tag="J1Y83_003612"
                     /EC_number="2.7.8.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418779.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerol--membrane-oligosaccharide
                     glycerophosphotransferase"
                     /protein_id="HBA7631724.1"
                     /translation="MSELLSFALFLASVLIYAWKAGRNTWWFAATLTVLGLFVVLNIT
                     LFASDYFTGDGINDAVLYTLTNSLTGAGVSKYILPGIGIVLGLAAVFGALGWILRRRR
                     HHPHHFGYSLLALLLALGSVDASPAFRQITELVKSQSRDGDPDFAAYYKEPSRTIPDP
                     KLNLVYIYGESLERTYFDNEAFPDLTPELGALKNEGLDFSHTQQLPGTDYTIAGMVAS
                     QCGIPLFAPFEGNASASVSSFFPQNICLGDILKNSGYQNYFVQGANLRFAGKDVFLKS
                     HGFDHLYGSEELKSVVADPHYRNDWGFYDDTVLDEAWKKFEELSRSGQRFSLFTLTVD
                     THHPDGFISRTCNRKKYDFDGKPNQSFSAVSCSQENIATFINKIKASPWFKDTVIVVS
                     SDHLAMNNTAWKYLNKQDRNNLFFVIRGDKPQQETLAVKRNTMDNGATVLDILGGDNY
                     LGLGRSSLSGQSMSEIFLNIKEKTLAWKPDIIRLWKFPKEMKEFTIDQQKNMIAFSGS
                     HFRLPLLLRVSDKRVEPLPESEYSAPLRFQLADFAPRDNFVWVDRCYKMAQLWAPELA
                     LSTDWCVSQGQLGGQQIVQHVDKAIWKGKTAFKDTVIDMARYKGNVDTLKIVDNDIRY
                     KADSFIFNVAGAPEEVKQFSGISRPESWGRWSNAQLGDEVKIEYKHPLPKKFDLVITA
                     KAYGNNASRPIPVRVGNEEQTLVLGNEVTTTTLHFDNPTDADTLVIVPPEPVSTNEGN
                     ILGHSPRKLGIGMVEIKVVEREG"
     gene            complement(9088..10110)
                     /locus_tag="J1Y83_003613"
     CDS             complement(9088..10110)
                     /locus_tag="J1Y83_003613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418778.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase family
                     protein"
                     /protein_id="HBA7631725.1"
                     /translation="MSTMKVLICQEIKNLVWEVRERPVPGDNEALIKIKTVGICGTDI
                     HAWAGNQPFFSYPRVLGHEICGEIIESGKNIHNFNIGQQVAVIPYISCQQCPACKSGR
                     TNCCEKISVIGVHQDGGFSEYLSVPVANILPADGIDPQAAALIEPFAISAHAVRRAAI
                     APSEQVLVVGAGPIGLGAAAIAKADGAQVVVADTSPARREHVATRLELPVLDPSAEDF
                     DAQLRAQFGGSLAQKVIDATGNQHAMNNTVNLIRHGGTVVFVGLFKGELQFSDPEFHK
                     KETTMMGSRNATPEDFAKVGRLMAEGKITADMMLTHRYPFATLAETYERDVINNRELI
                     KGVITF"
     gene            10249..11154
                     /locus_tag="J1Y83_003614"
     CDS             10249..11154
                     /locus_tag="J1Y83_003614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000091590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBA7631726.1"
                     /translation="MSRSQNLRHSVINQVIEDMARGNIPSPLPSQSGLAEMYNISRTT
                     VRHILQHLSACGVLTLVGKNYVIARKPEQNDGFDCITASLKEQNRLFEQAFFTMINLR
                     QLRAGESFSELQLARAASVSPVVVREYLLKFGRYNLIQSEKRGQWSMKQFDQSYAEQL
                     FELREMLETHSLQHFLNLPDDDPRWLQAKTMLERHRTLRDSIGSNFRMFSQLDRDFHA
                     MLLSAADNIFFNQSLEIISVIFHFHYQWDESDLKQRNIIAVDEHMTILSALICRSDLD
                     AITALRNHLDTAKQSMIRSIRQHHR"
BASE COUNT         2916 a         2861 c         2606 g         2808 t
ORIGIN      
        1 aagtcccccc cctcgcacca attatcttta cttcttttct tgtctcttca ctgttttatc
       61 acccctgatt caaattcatt atcatcagca tgatgccagc aataagcgcc aggcacgatg
      121 gtagcaaaac gtaatgccgt aactgtttgt gatacagcat caccggcgtc aataccaatc
      181 ccatcagaat gacaatccgc cattgatccg gcgataaatc cgcatacatc agccctgcaa
      241 tcagcaatcc cggcagcaac actccccagc cactgcccag cgtacgttgc aacatggttt
      301 catctctttc attgataatg ataactaata tcatatgatg atttttatca tttgcaagcc
      361 agagaaattc cttgctatcg ggtaaaccta tcgataggat tagcaataat tatcatttag
      421 attactatca cgattatggc ctatcgttcc gcaccgctct atgaagatgt tatctggcga
      481 acgcatctcc agccgcagga tgctgggctt gcacaagccg tgcgggcaac gatcgccgaa
      541 catcgtgaac atttgctgga gtttatccgc ctggatgaac ccgccccact taacgccatg
      601 acgctggcgc aatggtcatc accgaatgcg ctaagttctc tactcgcggt ctattccgat
      661 catatctacc gcaaccaacc tttgatgatc cgcgaaaaca aaccgcttat ctcactgtgg
      721 gcgcaatggt atatcggcct gatggtacca ccattaatgc tggcgctgct gactcaggaa
      781 aaagcattag atgtgtcgcc ggaacatgtc cacgttgagt ttcacgaaac cggacgcgcc
      841 gcctgtttct gggtcgatgt gtgtgaagat aaaaacgcaa cactacattc gccgcagcag
      901 cgaatggaaa cgttaatcag ccaggcgctg gttccggttg tgcaggcact agaagcaacg
      961 ggagagatta acggcaaact tatctggagc aataccggtt atttgatcaa ctggtatctg
     1021 actgagatga aacagctgct cggcgaggcg acggttgaat cgctgcgcta tgccctcttt
     1081 tttgagaaaa cgcttactac cggtgaagat aatccactct ggcgtaccgt ggtgctacgc
     1141 gacggcctgc tggtgcgccg cacttgctgc cagcgttatc gcttaccgga tgtgcagcaa
     1201 tgtggcgatt gtacgctgaa atgaaagcac tgccggggaa gtaaacccgg catcatgcgg
     1261 attaataagg atgcaacaca ttacttgttt cgcaatgccg catacataac agaatatctg
     1321 cggcctccaa caaccctgca tcagaactga cgcccagttt cgacatcaca ttaaatttat
     1381 gtgcacggat cgttttgata ttgcgtttaa gctgctcggc aatttgtgtc attgagtagc
     1441 cacgcgacat aaagcgcaat atttctctct ccgtcgggct taacgtccgg ctttggttaa
     1501 tgtaccactg attgttcagt cgatcggtcg cctgacgtac accatttaat gacaaaaaga
     1561 gttcctgatg aaaaatctcc agcgttgacg ctttacttaa tacaccgtcc agcggtgatg
     1621 gcgacaatga accaatcagc cgagcttcaa tatcatcatc cgcaataact aaacgccggg
     1681 tacgcggaaa cttaatcgcc agttcagtca ggcaggataa tccctctctg cgcgcacttc
     1741 tcatggcaga aaaagaaaaa atgaccgccg caaacgagat ccgcgacatg gcatcctgaa
     1801 atgactcctg actggtatag gtatggagct tataagggtt acccgcaaac tttctgaata
     1861 aactctcaat accgatacta ctcatgacgc atttttctat gagtgcgaca tttctcttct
     1921 taattcggct gtgttccatt ctgcggcatc tccataaaca gaaggcaagt taagttctct
     1981 tgcaccctgc gcgctattta tccatgcata catttcagcg ttgctgtgaa ttgccagtcg
     2041 acgcatcgca ctgttttttt gggcgctaat agttttatta cttttcttaa gcaatgaagc
     2101 tatctggttg atgccccatc ctttacctaa tagccgcagt accttgcgct ctgaaagagt
     2161 gagaatgacg gagcgatagc taaaatcatg agtttcagtc attgccgggg aggtcagcat
     2221 ggcgctgata cgctctgcgt gcgtattgcc ggaacgtacg gtcttgacca gattctcaat
     2281 gggttcaaca tcagacaata acgtcgccgt agggcacatg agcaattcta ctgcctggga
     2341 ataccaggag cgtgacacca tgaaaaccca gtgaatttcc cgatattgtg agattaaaga
     2401 gtaataatgc tcacaaacgg aacgtggatc ttcactttca ccggccaaat cagcaatgac
     2461 taatccggaa cgacgtaatt gtaataaggt aaggtcctcc gcagaggcgc tggatattat
     2521 ttcatattca gggaagtgag tcctcatgac ctcttttaac cctgcttgca taacaggtat
     2581 tttactgata acaattccat ttttgcagca ccctggcaac atatttcctc cacatccact
     2641 gtctaaaaat catgatggtt tattcaaggt attcattcct taaatattcc cagcaaatta
     2701 atagattaag cgaataaatt atccttagca ttcactttca tatcagcata ctcctgctgc
     2761 acaacaactc cactgcatgt gtcagaaggt tagcatcaaa tgataacctg gtgaaatagc
     2821 ctggagacat ttaaaatatc tacattaagt tgttagaaat acttaatgaa aaacagaaat
     2881 ctcattcatt taatttataa ttaaatgata tcccttcccg cagaatgcgg gaatcacaca
     2941 atcaatattt attcaataaa atctgatggt tttaacttgt taacattaaa aaacgcgccc
     3001 agctatctat tatcctgact cgtaaaggcc aaataacaaa ttcattatag tgataatatt
     3061 catgcgatag tttgtttacg gcgagagata attcgctttt tatcaccgag catctcccga
     3121 taaccaggat taatcagcgc aattttgatc ttaacctacc gcaaaacaac cgtgcttcat
     3181 gatacaaatg gattctcatt atctgaacaa tacgcagcac gtctatgaca aagggcgagt
     3241 tatgcaaact gagcaacagc gagccgtaac acggctttgt atccagtgtg gattatttct
     3301 tttgcaacat ggtgcggaaa gcgcgttggt tgatgagctt tcctcacgac tgggtcgggc
     3361 gttgggaatg gacagcgtcg aaagttctat ctcttcgaac gccatcgtgc tgacaaccat
     3421 taaagatggg caatgcctga catcgacacg taaaaatcac gatcgcggca ttaatatgca
     3481 tgtggtgact gaagtccagc acattgtaat tctggcggag catcatctgc tggattacaa
     3541 aggcgtagag aaacgattta gccaaattca gccattacgt tacccaagat ggctggtagc
     3601 cttaatggtt ggcctttcct gcgcctgttt ctgtaaactc aataacggtg gctgggatgg
     3661 tgccgtcatc accttctttg ccagtacggc cgcgatgtat atccgccagc tgctggcaca
     3721 acgtcatctt catccacaga tcaacttttg cctcaccgct ttcgccgcca ccaccatttc
     3781 cggattgctt ttgcaacttc ccactttcag caataccccc accattgcga tggccgccag
     3841 cgttctgctg ctggtgccgg gctttccgtt gataaatgcc gtcgccgata tgtttaaagg
     3901 ccacatcaat accggactgg cacgctgggc aatcgccagt ctgctgacac tggctacctg
     3961 tgtcggcgta gtaatggcac tgacgatttg ggggctacgc ggatgggtgt gatcgaattt
     4021 ctgttagcgt tggcgcagga tatgatcctc gccgccattc ctgcggtcgg ctttgcgatg
     4081 gtgttcaacg ttcccgttcg agcgttacgc tggtgtgcgc tgcttggtgc gataggtcat
     4141 ggttcccgaa tgatcttaat gaccagcggg ctgaatattg aatggtcaac ctttatggcc
     4201 tctatgctgg tcggcaccat tggtattcaa tggtcgcgct ggtatctggc gcatccgaaa
     4261 gtgtttaccg tggcggcggt tatccctatg ttccctggca tatcagctta taccgcaatg
     4321 atttcggcgg taaaaatcag ccagctaggt tacagcgaac cgttgatgat taccctgtta
     4381 accaactttc ttacagcttc atcgattgtt ggtgcgttat ccatcggtct ttccattcct
     4441 ggattatggt tgtaccgcaa gcgccctcgc gtataaaatt gcctcactta aagcacgcgg
     4501 atgagagaca gcctcctctc ctccgtgtgt tactataaaa gttatctccc ttctcgttca
     4561 tcgttccata tttgagaaac agtatgtctt ccagagtttt gaccccggac gtcgttggta
     4621 ttgacgccct ggtacacgat caccaaaccg ttctggcaaa agctgaaggc ggtgtggttg
     4681 ccgtatttga caacaacgcc ccggcgtttt atgccgtcac gcctgcacgc ctggctgaac
     4741 tgctggcgct agaagaaaag ctggcgcgtc cgggaagtga cgtggccctg gacgatcaac
     4801 tctatcagga accgcaaacc gctcccgttg ccgtacccat ggggaaattt gccatgtacc
     4861 cagactggca acccgatgcc gactttatcc gtcaggcggc attatggggc gtggcactaa
     4921 gagagccggt gaccgccgaa gaactggcgt cattcattgc ctactggcag gcggaaggca
     4981 aagtctttca ccatgtgcag tggcaacaaa aactggcgcg cagcctgcaa atcggtcgtg
     5041 ccagcaacgg cggactgccg aaacgagatg tgaatacggt cagtgaacct gacagccaaa
     5101 ttccaccagg attcagaggg taacgatgaa aaacgttggc gacctgatgc aacgcctgca
     5161 aaaaatgatg cctgcccata tcaaacctgc tttcaaaacg ggtgaagaac tactggcctg
     5221 gcagaaagaa caaggggcga tccgctccgc cgctctcgaa cgtgaaaatc gggcgatgaa
     5281 aatgcagcgc acctttaacc gctccggtat tcgtccactg catcagaact gctcctttga
     5341 gaactatcgc gttgagtgtg aagggcaaat gaatgcgtta agcaaagcgc gccagtatgt
     5401 cgaagagttt gacggcaaca tcgccagctt catcttttct ggcaagccag gaaccggcaa
     5461 aaaccatctg gcggcggcaa tctgcaacga gctgctgcta cgcggtaaat ccgtattgat
     5521 catcaccgtg gccgatatta tgtcggcgat gaaagatacc ttcaggaata gcggtaccag
     5581 cgaagaacaa ctgcttaacg atctgagtaa cgttgatctg ctggtgatcg atgagatcgg
     5641 cgtgcagacc gaatcgaaat acgaaaaagt gatcattaac cagatcgtcg atcgccgctc
     5701 ttcttccaaa cgcccaaccg ggatgctgac caacagtaat atggaagaga tgaccaagtt
     5761 gctgggcgaa cgcgtgatgg accgtatgcg cctgggtaac agtttgtggg tgatcttcaa
     5821 ctgggatagc taccgtagcc gtgtaacagg taaagagtat taatccccgt ttctggcggt
     5881 atactgcgac acaattcaac cttgagtaat gatgatgaaa actattaaac atcttctgtg
     5941 ctgtgccatt gccgccagcg cattaatttc caccggggtg catgctgcgt cctggaaaga
     6001 tgcgctctcc agcgccgcca gcgaacttgg caaccaaaac aacacgacac aggaaggcgg
     6061 ttggtcgctc gcgtcattaa ctaacttgct tagcagcggg aaccaggcct taagcgcaga
     6121 taacatgaac aacgccgcag gcattctgca atactgcgcg aagcaaaagc tggcttcggt
     6181 aaccgatgcc gaaaacatca agaatcaggt actggaaaaa ctgggcctga acagtgaaga
     6241 gcaaaaagaa gacaccaact atctggatgg cattcagggt ttgctgaaaa ccaaagatgg
     6301 tcagcaactc aatctggata atattggaac gactccgctg gcagaaaagg tgaaaaccaa
     6361 agcctgcgat ctggtgttaa agcaggggct gaacttcatt tcctgaaccg cgaaacgccg
     6421 cgttttagct gttacccggc tgacgcggct ctctcctgcc cttttccctg ctcctggacg
     6481 gttttacccc tgttacacgg gaagcgaccg cgatcaaaaa agtctgttta taaactaatt
     6541 cctaacaaca tcgcattttt ttgacactag aattgcagca atatggcgac gccattacat
     6601 tgtgcttctc aaaaaactta aacgttggct aaacggcccg gtttagtgag gatcgcccgt
     6661 tgtcagaact actctctttc gccctttttc tcgcctctgt gctgatttac gcatggaaag
     6721 cgggacgtaa cacctggtgg tttgcagcca cattaacggt actggggcta tttgtcgttt
     6781 taaatatcac cctgtttgcc agcgactatt ttactggcga tggtattaac gacgcggttc
     6841 tctatacctt aaccaacagc ctgaccggtg ctggtgtcag caaatacatc ctgcctggta
     6901 tcggcattgt gctggggctg gcggcggtgt tcggtgcgct gggctggatc ctgcgccgtc
     6961 gtcgccatca tccgcaccat tttggttaca gcctgctggc actcttactg gcgctgggtt
     7021 cagtggatgc cagcccggca tttcgtcaga taacggaact ggtgaaatcc cagtcacgcg
     7081 acggcgaccc ggactttgcg gcttattata aagagccgtc gagaactatc cctgacccga
     7141 aactcaacct ggtttatatc tacggcgaaa gtctcgagcg gacctatttt gataacgagg
     7201 ctttcccgga tctcacgcct gaactgggcg cgttgaaaaa tgaaggcctg gatttcagcc
     7261 acacgcagca gctgccggga acggattaca cgattgcggg catggtagct tctcagtgcg
     7321 gcataccgct gtttgcccca tttgaaggca acgcctccgc ctctgtctcc agcttcttcc
     7381 cgcagaacat ctgtctgggc gatattctga aaaactcggg ttatcagaac tatttcgtgc
     7441 agggcgcgaa tctgcgtttt gccggtaaag atgtgttcct gaaatcgcac ggcttcgacc
     7501 acttatacgg ctcagaagag ttgaaaagcg tggtggccga cccgcactat cgcaacgact
     7561 ggggattcta cgacgatacc gttctcgatg aagcgtggaa aaaatttgaa gagctttccc
     7621 gctcaggtca gcgattctca ctgtttaccc tgacagtcga tacccatcac ccggatggtt
     7681 ttatctcgcg cacctgtaac cgcaaaaaat atgattttga cggtaagccg aatcagtcat
     7741 tcagcgcggt aagttgcagc caggagaata ttgcgacgtt tatcaacaaa atcaaagcgt
     7801 caccgtggtt taaagatacc gtcatcgtcg tctcttctga ccatttagcg atgaacaata
     7861 cggcgtggaa ataccttaat aagcaggatc gcaataacct gttttttgtc atccgtggcg
     7921 acaagccgca gcaagagacg ctggcagtga agcgtaatac gatggataac ggcgcgacgg
     7981 tgctggacat tctcggtggc gataactatc tcggacttgg ccgtagcagt ttatccgggc
     8041 agtcgatgtc ggaaatcttc ctcaatatca aagagaaaac attggcgtgg aagccggata
     8101 tcatccgcct gtggaaattc cctaaagaga tgaaagagtt caccatcgac cagcagaaaa
     8161 acatgattgc cttctcaggt agccatttcc gtttgccgct gctgttgcgg gtttcagaca
     8221 aacgcgtgga accgctgccg gaaagcgaat actcagcacc gctgcgtttc cagctggcag
     8281 atttcgctcc acgcgacaat ttcgtctggg ttgaccgttg ctacaagatg gcacaactct
     8341 gggctccgga actggcactc tccaccgact ggtgtgtctc gcaagggcag cttggcggtc
     8401 agcaaattgt tcagcatgtt gacaaagcaa tatggaaggg caaaacggca tttaaagata
     8461 cggtcatcga catggcgcgt tacaaaggca atgtcgatac gctaaagatt gttgataacg
     8521 atattcgcta caaagccgac agtttcatct ttaacgtcgc tggtgcgcca gaagaggtga
     8581 aacagtttag cgggatttct cgtccagagt cgtggggccg ctggtccaac gcacagctgg
     8641 gcgatgaagt aaaaatcgag tacaagcatc cgctgccgaa gaaatttgac ctggtgatta
     8701 ccgccaaagc atacggcaat aacgccagcc gtcctattcc ggtacgcgta ggcaatgaag
     8761 aacaaaccct tgtgctgggc aatgaagtga ccaccaccac actgcatttc gataacccaa
     8821 ccgatgccga cacactggta attgtgccgc cggaacctgt ctcaaccaac gaagggaata
     8881 tcctcggaca ctcgccgcgt aagctcggga tcggcatggt ggaaattaaa gtggtagaac
     8941 gtgaagggta attcctgact gatgtgtagg tcgcataagg tgcgacctac acgccttctc
     9001 cggcttacaa cacattatta agaaacaaaa aaacagcgcc caacacttcc ccggatgctg
     9061 catggcaact tgaataatct cgccttatca gaaagtaatt acgcccttaa tcaactcacg
     9121 attgttaatc acatcgcgct cgtaggtttc tgccagcgtg gcgaacggat agcggtgggt
     9181 taacatcatg tcggcagtga ttttcccttc cgccatcaat cgaccgactt tagcgaaatc
     9241 ttccggcgtg gcgttgcggc tgcccatcat cgtcgtttct tttttatgga attccggatc
     9301 ggagaactgc aactcacctt taaacaggcc gacaaatacc accgtgccgc cgtgacgaat
     9361 cagatttacg gtgttattca tcgcatgttg attacctgtc gcgtcaatca ctttctgcgc
     9421 cagcgaacca ccaaactgcg cccgcagctg cgcgtcaaaa tcttcggctg acgggtccag
     9481 aacaggtaat tccagacgcg ttgccacatg ttcacggcgc gccggactgg tatccgccac
     9541 caccacctgc gcgccatcgg ctttagcgat tgccgccgcg cccagaccga ttggccccgc
     9601 cccgaccacc agcacctgct cgctgggtgc aatggctgca cgacgcaccg catgagcgct
     9661 aatagcgaaa ggttcaatca atgctgccgc ctgcgggtca ataccgtctg cgggcaaaat
     9721 gttcgccacc ggcacgctca aatactcact aaaaccgcca tcctgatgca cgccaatgac
     9781 tgaaattttt tcacagcaat tggtacgacc gcttttacac gccgggcatt gctgacaaga
     9841 aatatacgga attaccgcaa cttgctgtcc gatattaaaa ttgtgaatgt tttttcctga
     9901 ttcaataatc tccccacata tttcatggcc taaaacgcgt ggataactga aaaatggctg
     9961 attgcccgcc caggcatgaa tatctgtacc gcaaatcccg acagttttaa ttttaattaa
    10021 tgcttcatta tcgcctggca ccggtctttc ccggacttcc catactaaat tctttatttc
    10081 ctgacaaatt aatactttca ttgtggacat gattcaccgc tccttttatt ttatgacttt
    10141 cagatatcgt cgaatttata agagcgcctg aatttaccca taaaatatgc ggatgatcgc
    10201 gcataaaaat gttttaaatc ggcttttaat gccggactgg agggaagaat gagtcgttcg
    10261 cagaatttac gtcatagcgt cataaatcag gtgattgaag atatggcgag gggcaatatt
    10321 ccttctccgc tgccttcaca aagcggactg gcagaaatgt ataacatcag tcgcactacg
    10381 gtacgccaca tactccagca tttaagcgca tgtggcgtgc tcaccctggt ggggaaaaat
    10441 tatgttatcg cccgcaaacc cgaacagaac gatggatttg attgcatcac ggcctcgctg
    10501 aaagagcaaa accgtctttt cgagcaggca tttttcacca tgattaacct gcgccagtta
    10561 cgcgccggag aaagcttttc cgaacttcag ctggcgcgag cggcaagcgt cagtccggta
    10621 gtggtacgag aatatctttt gaaattcggt cgttataatc ttattcaaag cgaaaagcgc
    10681 ggccaatgga gcatgaagca gttcgatcaa tcctacgccg agcagctgtt tgagctacgc
    10741 gaaatgctcg aaacacattc cctgcaacac tttctcaacc ttcccgatga tgaccctcgc
    10801 tggctgcaag caaaaaccat gctcgaacgc caccggacgc tgcgcgacag cattggcagc
    10861 aactttcgaa tgttttctca attagaccgt gattttcatg ccatgctgtt atccgctgct
    10921 gacaacatct tttttaatca atcacttgaa attatatcgg tgatctttca ttttcactat
    10981 caatgggatg aaagcgatct caaacagcgc aatatcattg ctgttgatga acatatgacc
    11041 attctcagcg ccctgatttg ccgtagcgat ttagatgcaa taacggcgtt gcgaaaccat
    11101 ctggataccg ccaaacaatc aatgattcgt tccatcaggc aacatcaccg ataaattaag
    11161 tacagattaa aaacattgat tcttggttta t
//
LOCUS       DADSYM010000148        11145 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2415
            SAMN08039579-rid11723173.denovo.149, whole genome shotgun sequence.
ACCESSION   DADSYM010000148 DADSYM010000000
VERSION     DADSYM010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039579
            Sequence Read Archive: SRR6362533
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11145)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11145)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980162.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 03:39:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,484
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 4,347
            CDSs (with protein)               :: 4,347
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 43 of 85
            Pseudo Genes (incomplete)         :: 37 of 85
            Pseudo Genes (internal stop)      :: 19 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11145
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039579-rid11723173.denovo.149"
                     /strain="LGE2415"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8302 N 95.9628 W"
                     /collection_date="2015-08-24"
     gene            complement(15..836)
                     /gene="dapB"
                     /locus_tag="J1Y83_003615"
     CDS             complement(15..836)
                     /gene="dapB"
                     /locus_tag="J1Y83_003615"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HBA7631727.1"
                     /translation="MHDANIRVAIAGAGGRMGRQLIQAALALEGVQLGAALEREGSSL
                     LGSDAGELAGAGKTGVTVQSSLDAIKDDFDVFIDFTRPEGTLNHLAFCRQHGKGMVIG
                     TTGFDEAGKQAIRDAAADIAIVFAANFSVGVNVMLKLLEKAAKVMGDYTDIEIIEAHH
                     RHKVDAPSGTALAMGEAIAHALDKDLKDCAVYSREGHTGERVPGTIGFATVRAGDIVG
                     EHTAMFADIGERLEITHKASSRMTFANGAVRSALWLSGKESGLFDMRDVLDLNNL"
     gene            complement(997..1362)
                     /locus_tag="J1Y83_003616"
     CDS             complement(997..1362)
                     /locus_tag="J1Y83_003616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000220878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HBA7631728.1"
                     /translation="MTYGEAYLEGWKNIFNYEGVSNRFEFWSFMIGSGVICLLPLLCW
                     WLAVTINNDYGVFIFFALPASFILTLIFAIPAIALAVRRMHDIGYSGWWVTIVVLIPV
                     TGVILLILCCLPSKSQDQA"
     gene            complement(1385..2299)
                     /gene="rihC"
                     /locus_tag="J1Y83_003617"
     CDS             complement(1385..2299)
                     /gene="rihC"
                     /locus_tag="J1Y83_003617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001239167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside hydrolase RihC"
                     /protein_id="HBA7631729.1"
                     /translation="MRLPIFLDTDPGIDDAVAIAAAIFAPELDLQLMTTVAGNVSVEK
                     TTRNALQLLHFWNVDIPLAQGAAVPLVRAPRDAASVHGESGMAGYDFVEHNRQPLGIP
                     AFLAIRDALMRAPEPVTLVAIGPLTNIALLLSQCPECKPYIRRLVIMGGSAGRGNCTP
                     NAEFNIAADLEAAACVFRSGIEIVMCGLDVTNQAILTPDYLATLPELNRTGKMLHALF
                     SHYRSGSMQSGLRMHDLCAIAWLVRPELFTLKPCFVAVETQGEFTSGTTVVDIDGCLG
                     KPANVQVALDLDVKGFQQWVAEVLALAL"
     gene            complement(2365..3315)
                     /gene="ispH"
                     /locus_tag="J1Y83_003618"
                     /gene_synonym="lytB"
     CDS             complement(2365..3315)
                     /gene="ispH"
                     /locus_tag="J1Y83_003618"
                     /gene_synonym="lytB"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HBA7631730.1"
                     /translation="MQILLANPRGFCAGVDRAISIVENALAIYGAPIYVRHEVVHNRY
                     VVDSLRERGAIFIEQISEVPDGAILIFSAHGVSQAVRNEAKSRDLTVFDATCPLVTKV
                     HMEVARASRRGEESILIGHAGHPEVEGTMGQYSNPEGGMYLVESPDDVWKLTVKNEEK
                     LSFMTQTTLSVDDTSDVIDALRKRFPKIVGPRKDDICYATTNRQEAVRALAEQAEVVL
                     VVGSKNSSNSNRLAELAQRMGKRAFLIDDATDIQEEWVKEAKCVGVTAGASAPDILVQ
                     NVVARLQQLGGGEAIPLEGREENIVFEVPKELRVDIREVD"
     gene            complement(3317..3766)
                     /gene="fkpB"
                     /locus_tag="J1Y83_003619"
     CDS             complement(3317..3766)
                     /gene="fkpB"
                     /locus_tag="J1Y83_003619"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HBA7631731.1"
                     /translation="MSESVQSNSAVLVHFTLKLDDGTTAESTRNNGKPALFRLGDASL
                     SEGLEQHLLGLKVGDKTTFSLEPDAAFGVPSPDLIQYFSRREFMDAGEPEIGAIMLFT
                     AMDGSEMPGVIREINGDSITVDFNHPLAGQTVHFDIEVLEIDPALEA"
     gene            complement(3874..4368)
                     /gene="lspA"
                     /locus_tag="J1Y83_003620"
     CDS             complement(3874..4368)
                     /gene="lspA"
                     /locus_tag="J1Y83_003620"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein signal peptidase"
                     /protein_id="HBA7631732.1"
                     /translation="MSQSICSTGLRWLWLVVVVLIIDLGSKYLILQNFALGDTVPLFP
                     SLNLHYARNYGAAFSFLADSGGWQRWFFAGIAIGISVILAVMMYRSKATQKLNNIAYA
                     LIIGGALGNLFDRLWHGFVVDMIDFYVGDWHFATFNLADTAICVGAALIVLEGFLPSK
                     AKKQ"
     gene            complement(4368..7184)
                     /gene="ileS"
                     /locus_tag="J1Y83_003621"
     CDS             complement(4368..7184)
                     /gene="ileS"
                     /locus_tag="J1Y83_003621"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HBA7631733.1"
                     /translation="MSDYKSTLNLPETGFPMRGDLAKREPGMLARWTDDDLYGIIRAA
                     KKGKKTFILHDGPPYANGSIHIGHSVNKILKDIIIKSKGLSGYDSPYVPGWDCHGLPI
                     ELKVEQEYGKPGEKFTAAEFRAKCREYAATQVDGQRKDFIRLGVLGDWSHPYLTMDFK
                     TEANIIRALGKIIGNGHLHKGAKPVHWCVDCRSALAEAEVEYYDKTSPSIDVAFQAVD
                     QDALKTKFGVSNVNGPISLVIWTTTPWTLPANRAISIAPDFDYALVQIDGQAVILAKD
                     LVESVMQRIGVSDYTLLGTVKGAELELLRFTHPFMDFDVPAILGDHVTLDAGTGAVHT
                     APGHGPDDYVIGQKYGLETANPVGPDGTYLPGTYPTLDGVNVFKANDIVIALLQEKGA
                     LLHVEKTQHSYPCCWRHKTPIIFRATPQWFVSMDQKGLRAQSLKEIKGVQWIPDWGQA
                     RIESMVANRPDWCISRQRTWGVPMSLFVHKDTEELHPRTLELMEEVAKRVEVDGIQAW
                     WDLDAKEILGDEADQYVKVPDTLDVWFDSGSTHSSVVDVRPEFAGHAADMYLEGSDQH
                     RGWFMSSLMISTAMKGKAPYRQVLTHGFTVDGQGRKMSKSIGNTVSPQDVMNKLGADI
                     LRLWVASTDYTGEMAVSDEILKRAADSYRRIRNTARFLLANLNGFDPAKDMVKPEEMV
                     VLDRWAVGCAKAAQEDILKAYEAYDFHEVVQRLMRFCSVEMGSFYLDIIKDRQYTAKA
                     DSVARRSCQTALYHIAEALVRWMAPILSFTADEVWGYLPGEREKYVFTGEWYEGLFGL
                     ADSEAMNDAFWDELLKVRGEVNKVIEQARADKKVGGSLEAAVTLYAEPELAAKLTALG
                     DELRFVLLTSGATVADYNDAPADAQQSEVLKGLKVALSKAEGEKCPRCWHYTQDVGKV
                     AEHAEICGRCVSNVAGDGEKRKFA"
     gene            complement(7227..8168)
                     /gene="ribF"
                     /locus_tag="J1Y83_003622"
     CDS             complement(7227..8168)
                     /gene="ribF"
                     /locus_tag="J1Y83_003622"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HBA7631734.1"
                     /translation="MKLIRGIHNLSQAPQEGCVLTIGNFDGVHRGHRALLQGLQEEGR
                     KRNLPVMVMLFEPQPLELFATDKAPARLTRLREKLRYLAECGVDYVLCVRFDRRFAAL
                     TAQNFISDLLVKHLRVKFLAVGDDFRFGAGREGDFLLLQKAGMEYGFDITSTQTFCEG
                     GVRISSTAVRQALADDNLALAESLLGHPFAISGRVVHGDELGRTIGFPTANVPLRRQV
                     SPVKGVYAVEVLGLGEKPLPGVANIGTRPTVAGIRQQLEVHLLDVAMDLYGRHIQVVL
                     RKKIRNEQRFASLDELKAQIARDELTAREFFGLTKPA"
     gene            complement(8176..8394)
                     /locus_tag="J1Y83_003623"
     CDS             complement(8176..8394)
                     /locus_tag="J1Y83_003623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2575 domain-containing protein"
                     /protein_id="HBA7631735.1"
                     /translation="MCRHSLRSDGAGFYQLAGCEYSFSARKIAAGGQFLPMICAIAMK
                     SHFFLISVLNRRLTLTAVQGILGRFSLF"
     gene            8497..8760
                     /gene="rpsT"
                     /locus_tag="J1Y83_003624"
     CDS             8497..8760
                     /gene="rpsT"
                     /locus_tag="J1Y83_003624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006117633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HBA7631736.1"
                     /translation="MANIKSAKKRAIQSEKARKHNASRRSMMRTFIKKVYAAIEAGDK
                     AAAQKAFNEMQPIVDRQAAKGLIHKNKAARHKANLTAQINKLA"
     gene            complement(8856..9761)
                     /gene="nhaR"
                     /locus_tag="J1Y83_003625"
     CDS             complement(8856..9761)
                     /gene="nhaR"
                     /locus_tag="J1Y83_003625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional activator NhaR"
                     /protein_id="HBA7631737.1"
                     /translation="MSMSHINYNHLYYFWHVYKEGSVVGAAEALYLTPQTITGQIRAL
                     EERLQGKLFKRKGRGLEPSELGELVYRYADKMFTLSQEMLDIVNYRKESNLLFDVGVA
                     DALSKRLVSSVLDAAVVEDEQIHLRCFESTHEMLLEQLSQHKLDMIISDCPIDSTQQE
                     GLFSMKIGECGVSFWCTNPLPEKPFPACLEERRLLIPGRRSMLGRKLLNWFNSQGLNV
                     EILGEFDDAALMKAFGATHNAIFVAPSLYANDFYNDDSVVEIGRVENVMEEYHAIFAE
                     RMIQHPAVQRICNTDYSALFTPASK"
     gene            complement(9821..10987)
                     /gene="nhaA"
                     /locus_tag="J1Y83_003626"
     CDS             complement(9821..10987)
                     /gene="nhaA"
                     /locus_tag="J1Y83_003626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaA"
                     /protein_id="HBA7631738.1"
                     /translation="MKHLHRFFSSDASGGIILIIAAVLAMIMANSGATSGWYHDFLET
                     PVQLRVGTLEINKNMLLWINDALMAVFFLLVGLEVKRELMQGSLASLRQAAFPVIAAI
                     GGMIVPALLYLAFNYADPITREGWAIPAATDIAFALGVLALLGSRVPLALKIFLMALA
                     IIDDLGAIIIIALFYTNDLSMASLGVAAVAIAVLAVLNLCGVRRTGVYILVGVVLWTA
                     VLKSGVHATLAGVIVGFFIPLKEKHGRSPAKRLEHVLHPWVAYLILPLFAFANAGVSL
                     QGVTLEGLTSILPLGIIAGLLIGKPLGISLFCWLALRLKLAHLPEGTTYQQIMAVGIL
                     CGIGFTMSIFIASLAFGSVDPELINWAKLGILVGSISSAVIGYSWLRVRLRPSV"
BASE COUNT         2834 a         3099 c         2669 g         2543 t
ORIGIN      
        1 taaatatatt atggttacag attattgaga tcaagcacat ctcgcatatc aaaaagacca
       61 ctttccttac cactcaacca caaagccgat cttaccgcac cgttagcaaa tgtcatgcgg
      121 ctggatgcct tatgggtgat ctccagacgc tcgccaatat cggcaaacat cgcggtatgt
      181 tcgccaacga tgtcacctgc acgcacggtg gcaaaaccaa tggtgccagg cacacgttca
      241 ccggtatggc cttcacgact gtagaccgcg caatctttca gatctttatc aagagcgtgg
      301 gcgatcgcct ctcccattgc cagtgcggtg cctgacggcg catcaacttt atgtctatga
      361 tgtgcttcaa taatttcgat atcggtgtag tcccccatca ctttggctgc tttctccagc
      421 agtttaagca tgacgttaac gccaacgcta aaattggcgg caaagacaat cgcaatatcg
      481 gcggcggcgt cacgaatcgc ctgtttaccg gcttcgtcaa accccgtagt gccgatcacc
      541 atccctttgc catgctggcg acaaaaagcg agatggttca gcgtaccttc cggacgggta
      601 aaatcgataa acacatcaaa atcatctttt atcgcatcaa gactactttg cacagtaacg
      661 cctgttttcc cggctccggc cagttcgccc gcatcgctgc ccagtaaaga agatccttcg
      721 cgctccagcg ccgcgcccag ctgcacgcct tctaatgcca gcgccgcctg aatcaactgg
      781 cggcccatac ggcccccggc tcccgcgatg gcaacgcgga tgtttgcatc atgcatagct
      841 attctctttt gttaatttgc atagaccgtt ttcagagtaa ccagcgcatc gctacgccgc
      901 cagccaaaaa cgctgataat tagaaattaa tgataaacag ggagggatat cagtgaaagg
      961 catgacgcag aaagtgatgc gtcataccct ctgtcattat gcctggtctt gtgactttga
     1021 cggcaggcag cacagtataa gcagaattac gcccgtaaca gggatcaaca cgacaatggt
     1081 aacccaccac cctgaatatc cgatatcgtg catccttctg acggctaacg ctatcgccgg
     1141 tatggcaaaa atcaacgtta aaataaatga cgcgggcagc gcaaaaaaaa tgaaaacacc
     1201 atagtcattg ttaattgtta ccgctaacca ccaacataaa agcggaagca gacagataac
     1261 gccactacca atcataaacg accagaactc gaagcgattg gaaacacctt cataattaaa
     1321 gatgtttttc cacccttcca gatacgcttc tccatacgtc atagaacact tccttgtttt
     1381 attattagag cgccagggcc agcacctcag ccacccactg ctggaagcct ttcacatcca
     1441 gatccagcgc cacctgtaca ttggctggtt tgcccaggca accgtcgata tcaacaaccg
     1501 tcgtgcctga ggtaaattca ccctgagttt ctaccgcgac aaaacacggt ttgagagtga
     1561 acaattccgg gcgcaccagc caggcgatag cgcaaagatc gtgcatacgt agtccacttt
     1621 gcatactgcc gctacggtag tggctaaaca gggcgtgtag catttttccg gtgcgattca
     1681 attccggcaa agtagcgaga tagtcagggg ttaatattgc ctgattggtg acatccagac
     1741 cgcacatgac gatttcgatg ccgctacgga agacacaggc tgccgcttct agatcggcag
     1801 cgatattaaa ctcggcgttt ggtgtgcagt tgccgcgacc agcagaaccg cccatgatca
     1861 ccagacggcg aatatacggc ttacattccg ggcattgtga aagtaacagc gcaatattgg
     1921 ttaacgggcc gatggccacc agggtaacgg gctctggcgc acgcatcagg gcatcacgaa
     1981 ttgccagaaa cgccggtata cccagtggct gacggttgtg ttcaacaaag tcatagccag
     2041 ccattcccga ttcaccgtgt acagatgccg catcgcgcgg cgcacgaacc agtggtacgg
     2101 cagcgccctg ggcaagcgga atatccacat tccagaaatg cagcagttgc agggcgttgc
     2161 gggtggtttt ctctaccgaa acattacccg cgacagtggt catcagttgc agatcgagtt
     2221 cgggggcaaa aatcgcggcg gcaatggcaa cggcatcgtc aatgccgggg tctgtatcga
     2281 ggaagatagg taaacgcatg ttttctccat aaaaaatgcc ggtaacaaga ccggcatttt
     2341 cgcataactt aggctgctaa tgacttaatc gacttcacga atatcgacac gcagctcttt
     2401 cggcacttcg aaaacaatgt tttcttcacg accttccagc ggaatggctt cgccaccgcc
     2461 aagctgctgt aaacgtgcga ctacgttctg taccagaata tccggagctg atgcgccagc
     2521 agtcacgccg acgcatttcg cctctttcac ccactcttcc tggatatctg tcgcatcgtc
     2581 aatcaaaaac gcgcgcttgc ccatacgctg ggccagttcc gccagacggt tggagttgga
     2641 agagtttttc gaaccaacca ccagtactac ttccgcctgc tctgccaggg cgcgtactgc
     2701 ttcctgacgg ttagtcgtgg cgtagcagat gtcatctttg cgcggaccga caattttcgg
     2761 gaagcgttta cgcagcgcgt cgatcacatc agacgtgtca tccaccgaca gcgtggtttg
     2821 ggtcataaag gagagcttct cttcgttttt gaccgtcagt ttccacacat cgtctggcga
     2881 ttcgaccaga tacattcccc cttccgggtt gctgtactgg cccatcgtcc cttccacttc
     2941 cgggtgcccg gcgtgaccga tgagaataga ttcttcacca cgacggctgg cgcgggcgac
     3001 ttccatatgc actttggtca ccagcggaca ggtggcatca aacaccgtca aatcgcggct
     3061 tttcgcttcg ttacgtactg cctgagaaac accgtgtgcg gagaaaatca ggatcgcgcc
     3121 gtccggtact tcgctaatct gctcaataaa gatagcccca cgctcgcgca ggctatcgac
     3181 cacgtagcgg ttatgcacca cttcgtgacg gacatatatc ggtgcgccgt aaatggccag
     3241 cgcgttttca acaatgctga tagcgcggtc taccccggca caaaaaccac gcgggttggc
     3301 caacaggatc tgcatgttac gcctccagtg ccggatcgat ttccagcact tcaatatcaa
     3361 aatgaacggt ctgcccggcc agcggatggt tgaaatcaac ggtaatggag tcgccgttaa
     3421 tttcgcggat cacgccaggc atctcactgc catccattgc ggtaaaaagc atgattgcgc
     3481 caatttctgg ctcgcctgca tccataaatt cacggcggga gaagtactga atcaggtccg
     3541 gtgacggtac gccaaacgcc gcatctggct ccagcgagaa ggtggtttta tcgcccactt
     3601 tcagccccag caggtgttgc tccagccctt cagaaagaga agcatcaccc aggcggaaca
     3661 gcgccggttt accgttgttg cgggtagact cagcggtggt gccatcatcg agtttcagcg
     3721 tgaagtgcac caggacggcg ctattgctct gtacagattc agacatgcag gtttgctctt
     3781 atattttgag gatttttgcc ggatgcggcc tacgatttcg cagcaatctg taggccggat
     3841 aagatgcgtc agcatcgcat ccggcagggt ttattattgt tttttcgctt tagaaggtaa
     3901 aaaaccttcc agcacaatca gtgccgcacc gacacaaatg gcagtatcgg caaggttgaa
     3961 ggtggcgaag tgccagtcgc cgacatagaa gtcgatcata tcgacaacga agccgtgcca
     4021 caggcggtcg aacaggttgc ccagtgcgcc gccaataatc agcgcgtaag cgatattgtt
     4081 tagcttctgc gtggccttcg agcgatacat catcactgcc aggatcacgc taataccaat
     4141 cgcaataccg gcaaagaacc aacgctgcca gccgccgcta tcggcaagga aactaaacgc
     4201 tgcgccatag ttacgcgcat aatgcagatt aagcgacggg aacagcggga ccgtatcccc
     4261 cagagcaaag ttctggagga tcaggtattt gctgcccaga tcgataatca gcacgactac
     4321 caccagccac agccagcgta gccctgttga acagatcgat tgactcatca ggcaaactta
     4381 cgtttttcac cgtcaccggc gacgttgctg acacagcggc cgcagatttc tgcgtgttcc
     4441 gccaccttgc cgacatcctg ggtgtagtgc cagcagcgag ggcacttctc accttcggct
     4501 ttactcaacg cgactttcag ccctttgagg acttcgctct gctgggcgtc agcaggtgcg
     4561 tcgttatagt ctgcaacggt agcgccggag gtcaacagga caaatcgtaa ttcatcgccc
     4621 agcgcggtca gtttcgccgc cagttccggt tctgcataca aggttactgc cgcttccagc
     4681 gagccgccca ctttcttatc ggcacgcgct tgctcaatga ctttgttcac ttcgccacgc
     4741 actttcaaca gctcgtccca gaacgcatcg ttcattgctt cactgtctgc cagaccaaac
     4801 aggccttcgt accactcgcc ggtgaagacg tatttttcac gttcgcccgg caggtaaccc
     4861 cacacttcat cagcggtgaa ggagaggatt ggtgccatcc agcgaaccag cgcttctgcg
     4921 atgtgataca gcgctgtctg gcagctacga cgcgccacgc tgtccgcttt ggcggtatac
     4981 tgacggtctt tgatgatgtc gaggtagaag gaacccatct caacggagca gaagcgcatc
     5041 agacgctgta ccacttcgtg gaaatcgtat gcttcgtacg ccttgaggat gtcttcctgt
     5101 gccgctttcg cacaacctac ggcccagcga tccagtacca ccatctcttc cggtttcacc
     5161 atatcttttg ccggatcaaa accgttcagg tttgccagca ggaagcgcgc ggtgttacgg
     5221 atacgacgat agctgtcggc agcacgtttc aggatctcgt cagaaacggc catttcgcca
     5281 gtgtagtcgg ttgatgccac ccacagacgc agaatatccg cacccagttt gttcatcaca
     5341 tcctgcggcg aaacggtgtt accgatggat ttagacatct tgcgaccctg accatccacg
     5401 gtaaagccgt gagtcagtac ctgacgatat ggcgctttgc ccttcatcgc ggtagagatc
     5461 atcagggaag acatgaacca gccacggtgt tggtcagaac cttccagata catgtccgct
     5521 gcgtgaccgg caaattccgg acgcacatca acaacggaag agtgggtaga tccggagtca
     5581 aaccatacat ccagcgtatc cggtactttc acatactggt cagcttcgtc gccgaggatc
     5641 tctttcgcat cgagatccca ccacgcctga atgccgtcaa cttcaacgcg ttttgccact
     5701 tcttccatca gttcgagagt acgcggatgc agttcttctg tgtctttgtg cacgaacagt
     5761 gacatcggca cgccccaggt acgctgacga gagatacacc agtcaggacg gttagcaacc
     5821 atcgactcga tacgcgcctg gccccagtca gggatccact gcacgccttt gatctctttc
     5881 agtgactgcg cacgcagacc tttctgatcc atgctgacga accactgcgg cgtcgcgcgg
     5941 aagatgatcg gcgttttatg acgccagcag cacggatagc tgtgttgcgt tttctcaacg
     6001 tgcaacagtg cgcctttttc ctgcaacaac gcaatgacaa tatcgttcgc tttgaagacg
     6061 ttaacgccat ccagagtcgg gtaagtaccc ggcagataag tgccgtccgg gccaaccggg
     6121 ttagcggttt ccagaccata tttttgaccg atcacatagt cgtccggacc gtggcctggc
     6181 gcggtatgaa cggcaccggt accggcatcc agcgtaacgt ggtcgccgag aattgccgga
     6241 acatcgaagt ccataaacgg atgggtaaag cgcaacagtt ccagctcggc acctttcacc
     6301 gtgccaagaa gggtgtaatc gctaacgccg atacgctgca ttacgctttc aaccagatct
     6361 ttcgcgagga tcacggcctg accgtcgatt tgcaccagcg cataatcaaa atcaggtgca
     6421 atggagattg cgcggttagc aggcagcgtc cacggcgtgg tggtccatat aaccagcgaa
     6481 attgggccgt taacattgct tacgccaaat ttcgttttaa gcgcatcctg atcgaccgcc
     6541 tggaaagcga cgtcgatgga cggagaagtt ttgtcgtaat actcaacttc cgcttctgcc
     6601 agtgcagaac ggcagtcaac gcaccagtgc accggcttcg cgcctttgtg caggtgaccg
     6661 ttgccgatga ttttgcccag cgcgcggatg atgttggctt cagttttgaa gtccatggtc
     6721 aggtacgggt gcgaccagtc gcccagcacg cccagacgga taaagtcttt gcgctgaccg
     6781 tcaacctggg tcgcagcgta ttcgcggcac ttggcgcgga actcagcggc ggtgaatttc
     6841 tcccccggct taccgtattc ttgctctact ttcagttcga ttggcagacc atgacagtcc
     6901 cagccaggca catacggcga gtcatatcca gaaagccctt tggacttaat gataatgtct
     6961 ttcagaatct tgttaaccga gtgaccaata tgaatgctgc cattcgcata aggagggcca
     7021 tcatgcagaa tgaaggtttt tttgcctttt ttagctgcac gaatgatgcc gtacagatca
     7081 tcatcagtcc aacgcgccag cattcccggt tcgcgcttgg cgagatcgcc acgcatcggg
     7141 aaccctgttt ccggcaaatt cagggttgat ttatagtcac tcatcagatt ctcggttccg
     7201 tatttcggtt tgattacata acaggcttaa gccggttttg ttagcccaaa aaattcgcgg
     7261 gcggttaatt catcacgcgc aatctgcgct ttcagttcgt ccagcgatgc aaatcgctgc
     7321 tcattgcgta tttttttacg cagcactact tgtatatggc gaccataaag gtccattgca
     7381 acatctaaca aatgcacttc cagttgctgg cgaataccgg caaccgttgg gcgtgttccg
     7441 atgtttgcaa cgccgggtaa cggcttttcg ccaaggccca acacttctac cgcataaacc
     7501 cctttcaccg gggaaacctg acggcgtagc ggtacattcg ccgtcgggaa acctatagtg
     7561 cgccctaatt catcaccgtg gactacacgc ccggagatgg caaacgggtg ccccagtaaa
     7621 ctttctgcca gagccagatt gtcatccgcc agcgcctgac gcacggcggt gctgctgata
     7681 cgcacgccac cttcgcaaaa agtttgcgtg ctggtgatat cgaagccgta ttccatgccc
     7741 gctttctgta ataacaagaa atcgccttca cgaccagcgc caaagcggaa atcgtcacct
     7801 acggcaagaa attttacccg caagtgcttc accagaagat cactgatgaa gttttgcgcg
     7861 gttaacgccg caaaacgcct gtcaaaacgc acgcacagca cgtaatcaac accacactct
     7921 gcaagataac gcagtttttc ccgcagccgg gtgagccgtg ccggggcttt atcagtagca
     7981 aacagttcca gtggttgagg ttcaaaaagc atcaccatca ccggtaagtt gcgcttgcgc
     8041 ccttcttcct gcaagccctg taacagcgcg cgatgaccgc gatgcacgcc gtcgaaatta
     8101 ccaatagtca gcacacaccc ttcttgcggg gcctggctga gattatgtat gccgcgtatc
     8161 agcttcatgt ctggctcaaa acagtgaaaa tcgtccgagt ataccttgta cagcggtcaa
     8221 ggttaaccgg cgattgagta ccgaaatcag aaagaagtgg cttttcattg ctatggcgca
     8281 aatcatggga agaaactgac cgcctgctgc aatttttctc gcggaaaagc tgtattcaca
     8341 ccccgcaagc tggtagaatc ctgcgccatc actacgtaac gagtgccggc acattaacgg
     8401 cgcttatttg cacaaatcca ttgacaaaag aaggctaaaa gggcatattc ctcggccttt
     8461 gaattgtcca tatagaacac atttgggagt tggaccttgg ctaatatcaa atcagctaag
     8521 aagcgcgcca ttcagtctga aaaggctcgt aagcacaacg caagccgtcg ctctatgatg
     8581 cgtactttca tcaagaaagt atacgcagct atcgaagctg gcgacaaagc tgctgcacag
     8641 aaagcattta acgaaatgca accaatcgtg gaccgtcagg ctgctaaagg tctgatccac
     8701 aaaaacaaag ctgcacgtca taaggctaac ctgactgcac agatcaacaa actggcttaa
     8761 ttgcctgttg ttgtcagctt tgtaaaaaaa cccgctctgg cgggtttttt tatatccatc
     8821 tgcaggccaa aattcggcct gcaaagtaca aatgtttatt ttgaagctgg agtaaacagc
     8881 gcagaatagt ctgtattgca gatacgctgt actgcagggt gctgaatcat cctttcggca
     8941 aaaatcgcgt ggtactcttc catcacgttc tcaacacggc ctatctccac aaccgagtca
     9001 tcgttataga aatcattagc gtaaagcgaa ggtgcaacga aaatagcgtt atgcgtcgcc
     9061 ccaaaggctt tcatcaacgc agcatcatca aactcaccca aaatttcgac gttcaagccc
     9121 tgggagttaa accagtttaa taatttacgc cccaacattg agcgacgccc cggaataagt
     9181 aaacgacgct cttcaagaca ggcaggaaac ggcttttctg gtagtgggtt agtgcaccag
     9241 aaactgacac cacattcgcc aattttcatg gaaaacagcc cttcctgctg agtggaatcg
     9301 atcggacagt cagagatgat catatccagt ttatgctgac tcaactgctc taaaagcatc
     9361 tcgtgcgtcg attcgaaaca gcgtagatgg atctgctcgt cttccacaac tgcggcatcc
     9421 agaacactgc tgaccagacg tttggaaagt gcatctgcca caccaacatc aaacaataag
     9481 ttggactctt tgcgatagtt gacgatatcc agcatttcct ggcttaaggt gaacatttta
     9541 tcggcatagc gatagaccag ttcccccagt tcgctgggtt ccagaccacg tcctttacgc
     9601 ttaaatagtt tcccttgcag gcgctcttcc agcgcccgga tctgcccggt aatggtttgt
     9661 ggtgttaaat aaagcgcctc cgctgcgcca accacagaac cttctttgta gacatgccag
     9721 aagtaataca agtggttgta attgatatga gacatgctca tttctctccc tgataacaat
     9781 gaaaagggag ccgtttatgg ctccccagta catcgtcctg tcaaactgat ggacgtaaac
     9841 gaacgcgtaa ccagctatat ccaattaccg ccgaagaaat tgaaccgaca aggataccta
     9901 attttgccca gttaatcagt tctggatcta cgctaccaaa tgccaggctg gcaataaaga
     9961 tagacatagt aaaaccgata ccgcacagga taccaaccgc cataatttgc tggtaagtcg
    10021 ttccctctgg cagatgtgcc aatttcaaac gcagcgccaa ccagcagaac agactaatac
    10081 ccagtggctt gccaatcagc aaaccagcga tgatccctaa tggcagaatg gaggtcaaac
    10141 cttccagcgt gacaccttgc agtgaaacgc cagcattagc aaatgcaaac agcggcaaaa
    10201 tcagatacgc cacccatgga tgcaaaacat gctccagacg tttagccgga gagcgcccat
    10261 gcttctcttt caaaggaata aagaagccga caatgacgcc agccagggtt gcgtgaaccc
    10321 ccgatttcaa caccgctgtc cacagcacca cgccaaccag aatataaacg cccgtgcggc
    10381 gtacaccaca cagattcaat accgcgagta ccgcaattgc tacagccgcg acgccaagag
    10441 aggccatcga taagtcatta gtgtagaaca atgcgatgat aatgatggcc ccaagatcgt
    10501 cgataatagc cagagccatc aaaaagatct tcagcgctaa cggaacacga cttcccaaca
    10561 gcgccaacac accaagtgca aaggcaatgt cagtcgccgc cgggattgcc cagccttcgc
    10621 gggtaatcgg atcggcatag ttaaaagcca gatagagcaa tgccgggaca atcatcccgc
    10681 cgattgcggc aataacagga aatgccgcct ggcgcagact ggccagcgaa ccttgcatca
    10741 gctcgcgttt aacttccaga ccaaccaaca ggaaaaatac cgccatcaga gcgtcattga
    10801 tccatagcag catgttcttg ttgatctcaa gtgtcccaac ccggagctga accggcgtct
    10861 caagaaagtc gtgataccat ccactggttg caccgctgtt ggccataatc atcgctaata
    10921 cagcggcaat aatgagaata atgcctcccg aggcatcact gctaaagaat cgatgcagat
    10981 gtttcacttt ttatttctct tcaggtgaat agatcgtttt cacgatttta ccccgcacga
    11041 atcatcgtta aaatcagatt atagtgtagg aatagctcgc tttttacgat ctattaaaag
    11101 aattgcgcca tcagcgagag atgagttttt gatgcccgaa agcgg
//
LOCUS       DADSYM010000149        10914 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2415
            SAMN08039579-rid11723173.denovo.150, whole genome shotgun sequence.
ACCESSION   DADSYM010000149 DADSYM010000000
VERSION     DADSYM010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039579
            Sequence Read Archive: SRR6362533
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10914)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10914)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980162.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 03:39:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,484
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 4,347
            CDSs (with protein)               :: 4,347
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 43 of 85
            Pseudo Genes (incomplete)         :: 37 of 85
            Pseudo Genes (internal stop)      :: 19 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10914
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039579-rid11723173.denovo.150"
                     /strain="LGE2415"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8302 N 95.9628 W"
                     /collection_date="2015-08-24"
     gene            76..852
                     /gene="exuR"
                     /locus_tag="J1Y83_003627"
     CDS             76..852
                     /gene="exuR"
                     /locus_tag="J1Y83_003627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ExuR"
                     /protein_id="HBA7631739.1"
                     /translation="MEITEPRRLYQQLAADLKERIEQGVYLVGDKLPAERFIADEKNV
                     SRTVVREAIIMLEVEGYVEVRKGSGIHVVSNQPRHQQAADNNMEFANYGPFELLQARQ
                     LIESNIAEFAATQVTKQDIMKLMAIQEQARGEQCFRDSEWDLQFHIQVALATQNSALA
                     AIVEKMWTQRSHNPYWKKLHEHIDARTVDNWCDDHDQILKALIRKDPHAAKLAMWQHL
                     ENTKIMLFNETSDDFEFNADRYLFAENPVVHLDTATSGSK"
     gene            1197..1859
                     /locus_tag="J1Y83_003628"
     CDS             1197..1859
                     /locus_tag="J1Y83_003628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HBA7631740.1"
                     /translation="MELLTQLLQALWAQDFETLANPSMIGMLYFVLFVILFLENGLLP
                     AAFLPGDSLLVLVGVLIAKGAMGYPQTILLLTVAASLGCWVSYIQGRWLGNTRTVQNW
                     LSHLPAHYHQRAHHLFHKHGLSALLIGRFIAFVRTLLPTIAGLSGLNNARFQFFNWMS
                     GLLWVLILTTLGYMLGKTPVFLKYEDQLMSCLMLLPVVLLVFGLAGSLVVLWKKKYGN
                     RG"
     gene            1863..2246
                     /gene="mzrA"
                     /locus_tag="J1Y83_003629"
     CDS             1863..2246
                     /gene="mzrA"
                     /locus_tag="J1Y83_003629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EnvZ/OmpR regulon moderator MzrA"
                     /protein_id="HBA7631741.1"
                     /translation="MQIPRMSLRQLAWSGAVLLLVSTLLLAWSAVRQQESTLAIRAVH
                     QGTTMPDGFSIWHHLDAHGIPFKSITPKNDTLLITFDSSDQSAAAKAVLDRTLPHGYI
                     IAQQDNNSQAMQWLTRLRDNSHRFG"
     gene            2393..2761
                     /locus_tag="J1Y83_003630"
     CDS             2393..2761
                     /locus_tag="J1Y83_003630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1090 domain-containing protein"
                     /protein_id="HBA7631742.1"
                     /translation="MKYRIALAVSLFALSAGSYATTLCQEKEQNILKEISYAEKHQNQ
                     NRIDGLNKALSEVRANCSDSQLRADHQKKIAKQKDEVAERQQDLAEAKQKGDADKIAK
                     RERKLAEAQEELKKLKARDY"
     gene            2799..3104
                     /locus_tag="J1Y83_003631"
     CDS             2799..3104
                     /locus_tag="J1Y83_003631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF883 domain-containing protein"
                     /protein_id="HBA7631743.1"
                     /translation="MSKEHTTEHLRAELKSLSDTLEEVLSSSGEKSKEELSKIRSKAE
                     QALKQSRYRLGETGDAIAKQTRVAAARADEYVRENPWTGVGIGAAIGVVLGVLLSRR"
     gene            3107..3511
                     /locus_tag="J1Y83_003632"
     CDS             3107..3511
                     /locus_tag="J1Y83_003632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA7631744.1"
                     /translation="MADTHHAQGPGKSVLGIGQRIVSIMVEMVETRLRLAVVELEEEK
                     ANLFQLLLMLGLTMLFAAFGLMSLMVLVIWAVDPQYRLNAMIATTVVLLLLALIGGIW
                     TLRKSRKSTLLRHTRHELANDRQLLEEESREQ"
     gene            3501..3800
                     /locus_tag="J1Y83_003633"
     CDS             3501..3800
                     /locus_tag="J1Y83_003633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA7631745.1"
                     /translation="MSSKVERERRKAQLLSQIQQQRLDLSASRREWLEATGAYDRRWN
                     MLLSLRSWALVGSSVMAIWTIRHPNMLVRWARRGFGVWSAWRLVKTTLKQQQLRG"
     gene            3897..4379
                     /locus_tag="J1Y83_003634"
     CDS             3897..4379
                     /locus_tag="J1Y83_003634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HBA7631746.1"
                     /translation="MILSIDSNDANSAPLHKKTISSLSGAVESMMKKLEDVGVLVARI
                     LMPILFITAGWGKITGYAGTQQYMEAMGVPGFMLPLVILLEFGGGLAILFGFLTRTTA
                     LFTAGFTLLTAFLFHSNFAEGVNSLMFMKNLTISGGFLLLAITGPGAYSIDRLLNKKW
                     "
     gene            4449..5435
                     /locus_tag="J1Y83_003635"
     CDS             4449..5435
                     /locus_tag="J1Y83_003635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HBA7631747.1"
                     /translation="MGQLIDGVWHDTWYDTKSTGGKFQRSASAFRNWLTADGAPGPTG
                     KGGFAAEKDRYHLYVSLACPWAHRTLIMRKLKGLEPFISVSVVNPLMLENGWTFDDSF
                     PGATGDTLYQHEFLYQLYLHADPHYSGRVTVPVLWDKKNHTIVSNESAEIIRMFNTAF
                     DALGAKAGDYYPPALQPKIDELNGWIYDTVNNGVYKAGFATSQQAYDEAVAKVFESLA
                     RLEQILGQHRYLTGNQLTEADIRLWTTLVRFDPVYVTHFKCDKHRISDYLNLYGFLRD
                     IYQMPGIAETVNFDHIRNHYFRSHKTINPTGIISIGPWQDLDEPHGRDVRFG"
     gene            5718..6086
                     /locus_tag="J1Y83_003636"
     CDS             5718..6086
                     /locus_tag="J1Y83_003636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HBA7631748.1"
                     /translation="MDWYLKVLKNYVGFRGRARRKEYWMFILVNIIFTFVLGLLDKML
                     GWQRAGGEGILTTIYGILVFLPWWAVQFRRLHDTDRSAWWALLFLIPFIGWLIIIVFN
                     CQAGTPGENRFGPDPKLEQE"
     gene            complement(6120..7016)
                     /locus_tag="J1Y83_003637"
     CDS             complement(6120..7016)
                     /locus_tag="J1Y83_003637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBA7631749.1"
                     /translation="MAKERALTLEALRVMDAIDRRGSFAAAADELGRVPSALSYTMQK
                     LEEELDVVLFDRSGHRTKFTNVGRMLLERGRVLLEAADKLTTDAEALARGWETHLTIV
                     TEALVPTPAFFPLIDKLAAKANTQLAIITEVLAGAWERLEQGRADIVIAPDMHFRSSS
                     EINSRKLYTLMNVYVAAPDHPIHQEPEPLSEVTRVKYRGIAVADTARERPVLTVQLLD
                     KQPRLTVSTIEDKRQALLAGLGVATMPYPMVEKDIAEGRLRVVSPESTSEIDIIMAWR
                     RDSMGEAKSWCLREIPKLFSGK"
     gene            7121..7822
                     /locus_tag="J1Y83_003638"
     CDS             7121..7822
                     /locus_tag="J1Y83_003638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HBA7631750.1"
                     /translation="MITTRTARQCGQADYGWLQARYTFSFGHYFDPKLLGYASLRVLN
                     QEVLAPGAAFQPRTYPKVDILNVILDGEAEYRDSEGNHVQASAGEVLLLSTQPGVSYS
                     EHNLSKDKPLTRMQLWLDACPQRENPLIQKLALNMSKQQLIASPEGGMGSLQLRQQVW
                     LHHIVLDKGESANFQLHGPRAYLQSIHGKFHALTHHEEKAALTCGDGAFIRDEANITL
                     VADSPLRALLIDLPV"
     gene            7845..8009
                     /locus_tag="J1Y83_003639"
     CDS             7845..8009
                     /locus_tag="J1Y83_003639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA7631751.1"
                     /translation="MSKKSAKKRQPVKPVVAKEPARTAKNFGYEEMLSELEAIVADAE
                     TRLAEDEATA"
     gene            complement(8070..9380)
                     /locus_tag="J1Y83_003640"
     CDS             complement(8070..9380)
                     /locus_tag="J1Y83_003640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine dehydratase subunit alpha family protein"
                     /protein_id="HBA7631752.1"
                     /translation="MFDSTLNPLWQRYILAVQEEVKPALGCTEPISLALAAAVAAAEL
                     EGPVERVEAWVSPNLMKNGLGVTVPGTGMVGLPIAAALGALGGNANAGLEVLKDATAQ
                     AISDAKALLAAGKVSVKIQEPCDEILFSRAKVWNGEKWACVTIVGGHTNIVHIETHNG
                     VVFTQQACVTEGEQESPLTVLSRTTLAEILKFVNEVPFAAIRFILDSAKLNCALSQEG
                     LSGNWGLHIGATLEKQCARGLLAKDLSSSIVIRTSAASDARMGGATLPAMSNSGSGNQ
                     GITATMPVVVVAEHFGADDERLARALMLSHLSAIYIHNQLPRLSALCAATTAAMGAAA
                     GMAWLVDGRYETISMAISSMIGDVSGMICDGASNSCAMKVSTSASAAWKAVLMALDDT
                     AVTGNEGIVAHDVEQSIANLCALASHSMQQTDRQIIEIMASKAR"
     gene            complement(9408..10739)
                     /locus_tag="J1Y83_003641"
     CDS             complement(9408..10739)
                     /locus_tag="J1Y83_003641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026203.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAAAP family serine/threonine permease"
                     /protein_id="HBA7631753.1"
                     /translation="MEIASNKGVIADASTPAGRAGMSESEWREAIKFDSTDTGWVIMS
                     IGMAIGAGIVFLPVQVGLMGLWVFLLSSVIGYPAMYLFQRLFINTLAESPECKDYPSV
                     ISGYLGKNWGILLGALYFVMLVIWMFVYSTAITNDSASYLHTFGVTEGLLSDSPFYGL
                     VLICILVAISSRGEKLLFKISTGMVLTKLLVVAALGVSMVGMWHLYNVGSLPPLGLLV
                     KNAIITLPFTLTSILFIQTLSPMVISYRSREKSIEVARHKALRAMNIAFGILFVTVFF
                     YAVSFTLAMGHDEAVKAYEQNISALAIAAQFISGDGAAWVKVVSVILNIFAVMTAFFG
                     VYLGFREATQGIVMNILRRKIPAEKINENLVQRGIMIFAILLAWSAIVLNAPVLSFTS
                     ICSPIFGMVGCLIPAWLVYKVPALHKYKGMSLYLIIVTGLLLCVSPFLAFS"
BASE COUNT         2744 a         2879 c         2698 g         2593 t
ORIGIN      
        1 tatattttaa caatggcata cgcatgcata aagtggtatg ataactttgt ccgcattcca
       61 ctcctggaga cgcatatgga aatcactgaa ccacgccgtt tgtatcaaca acttgccgct
      121 gatctgaaag agcgcatcga acagggcgtc tatctggtgg gtgataaact gcctgctgag
      181 cgctttattg ccgatgaaaa aaacgtcagc cgcaccgtgg ttcgtgaagc catcatcatg
      241 ctggaagttg aaggctatgt cgaagtgcgt aaaggttcag gtattcatgt ggtttccaac
      301 cagcctcgcc atcagcaggc ggctgacaat aatatggaat tcgccaatta cggtccgttt
      361 gagttgctcc aggctcgcca gctcatcgaa agtaatattg ccgagttcgc ggcaactcag
      421 gtcacaaaac aggacatcat gaaattgatg gccattcagg agcaggcacg cggcgaacaa
      481 tgcttccgtg attccgagtg ggatttgcag ttccatattc aggtagccct ggcgacgcag
      541 aactccgccc tggcggctat cgttgaaaaa atgtggaccc agcgtagtca taacccgtac
      601 tggaaaaaac tgcacgaaca cattgatgcc cgtaccgtcg ataactggtg tgatgaccac
      661 gatcaaatcc tcaaggcgct gattcgcaaa gatcctcatg ctgctaagct ggcaatgtgg
      721 cagcacctgg aaaacaccaa gatcatgtta tttaacgaaa ccagcgacga tttcgagttc
      781 aatgccgacc gctatctgtt cgccgaaaac ccggtggtgc atctcgatac agccaccagc
      841 ggcagtaaat gaaattcctg tccgacaggc gctacagtgc gcctgtttgt ctcgtcggta
      901 agcgaaaagt ataaagtgtc agcctgtgta aatcctctcg cctccctccc ctgcattcag
      961 caaaatcaga ctccacggac atgtaatttt gataacgaac aacgttgacc tttgttacaa
     1021 ttagactcaa tttgaattta tgcttttgaa tgctttctta tctcacgatt taacagggaa
     1081 tagttcaggc tgtgttgatg tatcaaaccc gcagaacata ccaagacagc aataacattg
     1141 cggtagtgca tcatttaaaa ccagcgtggc gttaaccgat tcaccaggaa taatgaatgg
     1201 aacttttgac ccaattgctg caagccctgt gggcgcagga ttttgaaacc ctggccaatc
     1261 cgtcgatgat tggcatgttg tattttgtct tgtttgtcat tttgttcctt gaaaacggct
     1321 tgcttccggc ggccttttta ccgggcgaca gtttactggt actggtcggc gtattgattg
     1381 cgaaaggcgc gatgggctat ccgcaaacga ttctgctgct gaccgttgcc gccagcctcg
     1441 gttgctgggt cagctatatt caggggcgat ggctgggcaa tacccgcacc gtacaaaact
     1501 ggctatctca tttacccgcg cattatcatc aacgcgcaca ccatcttttt cataaacacg
     1561 gtttatcggc gctgttaatt ggtcgcttta ttgcgtttgt cagaacactg ctgccgacga
     1621 ttgccgggtt atcagggctg aataacgcgc gctttcagtt cttcaactgg atgagtggtc
     1681 tgctgtgggt gttgatcctg acaactctgg gttacatgct cggcaaaacg ccggtatttt
     1741 taaagtacga ggaccagctg atgtcatgcc tgatgctgct cccggtggtg ctgctggtgt
     1801 ttggcctggc aggttctctg gtcgtgttat ggaaaaagaa atatggaaat cgggggtaag
     1861 ggatgcaaat acctcgcatg tcacttcgcc agctagcctg gtccggcgct gttttacttc
     1921 tggtcagcac actattgctg gcctggtccg cggttcgcca gcaagagtct acgctggcga
     1981 ttcgtgccgt tcatcaaggg acaacgatgc cagacggttt ttcaatctgg catcaccttg
     2041 acgctcacgg cattcctttc aaaagtatca cccccaaaaa cgacactctg ttaattacct
     2101 ttgactccag cgaccagagc gccgccgcaa aagcggtcct cgacagaaca ttgccccatg
     2161 gctacatcat tgcgcagcag gacaataaca gtcaggctat gcagtggctg acccggttgc
     2221 gggataactc tcatcgcttc ggataacttc ctgttatccg aaacagatca ctcactttgg
     2281 tgatttcacc gtaactgtct atgattaatg aggcggtggt taacacgacc gcttcgttgt
     2341 ctggacaatc gttcctttgt aataggtccg aatcacaatg gaaggttcaa gaatgaaata
     2401 ccgcatcgct ttagctgttt ctctctttgc tcttagtgcc ggtagttatg ccactaccct
     2461 gtgtcaggaa aaggagcaaa atatccttaa ggagatcagc tatgccgaaa aacaccaaaa
     2521 ccagaatcgt attgacggtc tgaataaagc cctgagtgaa gtccgggcca actgttcaga
     2581 tagccagctg cgtgccgatc atcagaagaa aatcgcaaag cagaaagatg aggtggcgga
     2641 acgccagcaa gatttagccg aggcgaagca aaaaggcgat gcagataaga ttgccaaacg
     2701 cgaacggaaa ctggcagaag cgcaggaaga gctgaaaaag ctgaaagcgc gcgactacta
     2761 actcacaata gtcactactt actcacctgg agaaaactat gtcgaaagaa cacactacgg
     2821 aacatctgcg tgctgagttg aaatcccttt ccgatacgct ggaagaggtg cttagctcat
     2881 ctggcgagaa gtcaaaagaa gagttgagta agattcgtag caaagcggag caggcactga
     2941 aacagagccg ttatcgcctg ggtgaaaccg gtgatgccat tgccaaacaa acccgtgtcg
     3001 cggcggcgcg tgccgatgag tatgtgcgtg aaaatccgtg gacgggcgtg ggcattggcg
     3061 cagcaatcgg tgtagtgctc ggcgttctgc tgtcgcgtcg ttaattatgg cggacactca
     3121 tcacgcgcaa gggcccggta aaagcgttct gggcatcggg cagcgaattg tttccatcat
     3181 ggttgaaatg gtggagacac gtctgcggct ggcggtggtg gagctggaag aggaaaaagc
     3241 gaatctcttt caacttttac tgatgctggg cctgacgatg cttttcgctg catttggtct
     3301 tatgagcctg atggtgctag ttatctgggc ggttgatccg caatatcgcc tgaatgcgat
     3361 gattgccacc accgtggtgt tgctgctact ggcactgatt ggcggcatct ggacgttacg
     3421 taaatcgcgt aagtctacgt tgctgcgcca cacgcgccat gagctggcaa acgatcggca
     3481 gctgctcgag gaggagtccc gtgagcagta aagtcgaacg tgaacgacgt aaggcgcaac
     3541 tgcttagcca gatccagcaa caacggctgg atctttccgc cagtcgtcgt gaatggctgg
     3601 aggcaacagg cgcttacgat cgtcgctgga atatgctgtt gagtctgcgc tcctgggcgc
     3661 tggttggcag tagcgtgatg gctatctgga cgattcgcca tcctaatatg ctggtccgct
     3721 gggccagacg cggttttggt gtatggagcg cctggcgtct ggtgaaaaca accctcaagc
     3781 aacaacagct tcgcggttaa cttcctctct ggccggagaa attccggcct tatccctcaa
     3841 attttttgaa aatatttgac agttttcctt gctaacaatc acgcttcgtc acgtttatga
     3901 ttctctccat cgacagcaat gacgctaata gcgcgccctt gcacaaaaaa acaatcagca
     3961 gcctgagcgg cgcagtggag agtatgatga aaaaattaga agatgttggt gtactggtag
     4021 cgcgcatttt aatgccgatt ctgtttatta ccgcaggctg gggaaaaatt actggttacg
     4081 cgggtaccca acaatatatg gaagcgatgg gcgtcccggg ttttatgctg ccgctggtga
     4141 ttctgcttga gtttggtggt ggtctggcaa tcctgttcgg tttcctgact cgcaccacag
     4201 ccctgtttac tgcgggcttt acgctgctga cggcattttt atttcacagc aactttgctg
     4261 aaggcgtcaa ctcgctgatg ttcatgaaaa acctgacaat ttctggcgga ttcctgctgc
     4321 tggcaattac cggtccgggc gcgtatagca tcgaccgcct gctgaataaa aagtggtaag
     4381 cacgcgctat acttaacgat aaaaaacgag gaggaagctc ctcgtttttg ctattggagg
     4441 agagaaatat gggtcaactg attgacggcg tctggcatga cacctggtac gataccaaat
     4501 ccaccggcgg taaatttcaa cgttcagctt ccgcatttcg taactggctc actgccgatg
     4561 gcgcgccagg ccccactggc aaaggcggtt ttgccgcaga gaaagatcgt tatcatctct
     4621 atgtttcact cgcctgcccg tgggcacacc gcacgctgat catgcgtaag ctcaaaggac
     4681 tggaaccgtt tatttccgtt tccgtagtga acccgctgat gctggaaaac ggctggacct
     4741 ttgatgacag ttttccggga gcaaccggcg acacgctcta tcaacatgaa tttctgtatc
     4801 agctttatct ccacgccgat ccacactaca gcggacgagt gactgttccc gtgctgtggg
     4861 acaaaaaaaa ccacaccatc gtcagcaacg aatcagcaga aatcatacgc atgttcaata
     4921 ccgcgtttga tgcgctgggt gcgaaagctg gagattacta cccaccagcc ctgcaaccga
     4981 aaattgacga acttaacggc tggatttacg acaccgttaa caatggcgtg tataaagccg
     5041 gttttgccac cagccagcaa gcctacgacg aggcggtggc gaaagtgttt gaatcgctgg
     5101 cgcgactgga acagatttta ggtcagcacc gttacctgac cggcaaccag ctaaccgaag
     5161 ccgatattcg cctgtggacc acgctggtgc gttttgatcc agtgtatgtg acccacttca
     5221 agtgtgataa gcaccgcatc agcgattacc tgaatctgta tggcttcctg cgcgatatct
     5281 accagatgcc gggaattgcc gaaacagtca atttcgatca catccgtaat cattacttcc
     5341 gcagccataa gaccatcaac cctacgggga ttatttcaat tggtccgtgg caggatctcg
     5401 atgaaccgca tggacgagat gttcgcttcg gttaaaaaaa ggccccttac gaggtgcctc
     5461 tgataattag ctaagtacgt gttgttcata tcggatgcgg cgtaaacgcc ttatcaaacc
     5521 tacaaaacat ttatattcaa tatgttgcaa tgaccgcgca ggccagataa gcgtagcgca
     5581 tctggcaatt ttgcgcttgc ccttatcctt cacagcgcct tattaattca tcgcgataat
     5641 atttactgcc atttcattca cgtctattct taatttgctg ctttaaaaac aagtgattga
     5701 gcaaaattga ggcaaaaatg gactggtatc tgaaagtact aaaaaattat gtcggtttcc
     5761 ggggtcgtgc gcggcgcaaa gagtactgga tgtttattct ggtcaacatc atctttacgt
     5821 tcgtgctggg gctactggat aaaatgttag gctggcaacg tgccggtggc gaaggcatcc
     5881 tgacgacaat ctacggtatt ctggtgtttt taccatggtg ggcagttcag ttccgccgcc
     5941 tgcacgacac cgaccgctcg gcgtggtggg cactgctatt cttaatcccg ttcatcggct
     6001 ggcttattat catcgtcttt aactgccagg cgggtacgcc aggcgaaaac cgctttggac
     6061 cagatccgaa actggaacaa gaataataat taagcacctt gtaaaaggtg cttaatcgat
     6121 tattttccgc taaaaagttt gggaatttcc cgcagacacc aggatttcgc ctcccccata
     6181 ctgtcacgac gccaggccat aataatatcg atctcgctgg tcgattccgg gctgacgaca
     6241 cgcaaccgcc cttccgcaat atctttttcg accatcggat acggcatcgt cgccacgcca
     6301 agccccgcca gtaatgcctg acgtttatct tcaattgtgc tcaccgttaa gcgcggctgt
     6361 ttgtccagca actgtacggt caacaccggg cgctcacgag cggtatccgc caccgcaatt
     6421 ccacgatatt tcacgcgcgt cacttcagat aacggttccg gctcctgatg aatcgggtga
     6481 tccggcgcgg caacatagac gttcattaag gtatagagtt tgcgcgagtt gatctccgac
     6541 gaggaacgaa aatgcatatc cggcgcgata acgatatccg cccgcccctg ctccagccgt
     6601 tcccacgccc ccgccagcac ttcggtgatg attgccagtt gggtattggc ttttgccgcc
     6661 agtttgtcga ttaacgggaa aaaggcaggt gtcggtacca gcgcctcggt cacaatggtg
     6721 agatgcgttt cccaaccacg cgcgagggct tccgcatcgg tagtcagttt atctgcggct
     6781 tccagcaaaa cgcgccctcg ttccagcaac atcctcccga cattggtgaa tttggtgcga
     6841 tggcccgagc ggtcaaacag caccacatcc agctcttctt ccagtttttg catggtgtag
     6901 ctaagtgcgg aaggcacgcg tcccagctca tccgccgccg ccgcaaaact gccccggcga
     6961 tcgatcgcat ccataacccg tagtgcttcc agcgttaatg ccctttcttt ggccatttcg
     7021 ttctcattca ggaaatttga acataccggg cagaatatct ggctaacaat gtagcgtcca
     7081 gccccttacc ataaaaggaa gtaaagagag gtcaagaatt atgattacta cccgaactgc
     7141 caggcagtgt ggacaagcag actacggatg gttgcaggcc cggtatactt tttcctttgg
     7201 acactacttc gacccgaaat tgttaggcta tgcctccctg cgcgtgctta accaggaagt
     7261 gctggcccca ggtgccgcct ttcagccgcg aacctatccc aaagtcgata ttttaaatgt
     7321 gattctggat ggagaagctg agtatcgcga cagcgaaggc aatcatgttc aggccagcgc
     7381 cggtgaggtg ttgctgctct ctacccagcc gggtgtcagc tatagcgaac acaatctcag
     7441 caaagacaaa ccgttaacgc gaatgcagct ttggctggat gcctgcccgc agcgagagaa
     7501 tccgctgatt caaaagctgg cgcttaatat gagcaagcag caattaatcg cctcgccaga
     7561 aggtgggatg ggaagcctgc aattgcgcca gcaagtgtgg ctgcaccata tcgtgctcga
     7621 caaaggcgaa agtgcgaatt tccagttgca tgggccacgc gcgtatttgc aatcgattca
     7681 cgggaaattt catgcgctta cgcatcatga agagaaagcg gcgctaacct gcggtgatgg
     7741 ggcgtttatt cgtgacgagg ctaacattac gctggttgcc gattccccac tgcgcgcttt
     7801 gctgatagat ttgcctgtct agttgttttt acagggagat gatgatgagt aaaaaatcgg
     7861 ccaaaaagcg ccagccagtg aagcccgtgg tggcgaaaga acctgctcgt accgccaaaa
     7921 attttggcta tgaagagatg ttgagcgagc tggaagccat cgtcgcggat gctgaaacgc
     7981 gtttagccga agatgaagct accgcgtaaa gacaagttgc cggatgcggc gtaaacgcct
     8041 tatccggcac aatacccaaa acctggcgtt tatctggcct tgctcgccat aatctcgata
     8101 atctgccgat ccgtttgctg catcgaatgg cttgctaacg cacacaggtt ggcaatcgac
     8161 tgctcaacat catgcgccac aatcccttca ttaccggtta cggcggtatc atccagcgcc
     8221 attaacaccg ctttccacgc agccgaagca ctggtcgaaa ccttcatcgc gcagctgttc
     8281 gatgcaccat cgcaaatcat gccgctgaca tcgccgatca tactgctgat cgccatcgag
     8341 atggtttcat aacgcccatc caccagccat gccatcccgg cagcggcccc cattgctgcg
     8401 gtcgttgcgg cacacagcgc agacaaacgc ggtaactggt tatggatgta aatcgcgctc
     8461 aaatgcgaaa gcatcagcgc acgcgccagc cgttcatcat ccgctccgaa gtgttcagct
     8521 accaccacca cgggcattgt tgcggtgatc ccctggttac ccgagccgga gttactcatt
     8581 gccggaagcg tagcgccgcc catacgcgca tcggatgccg cgctggtacg aatcacaatg
     8641 gatgaagaga gatctttcgc cagcaaaccg cgcgcgcact gtttttccag cgtcgcacca
     8701 atatgcagcc cccagttacc gctcaaacct tcctgcgata acgcacaatt taacttcgca
     8761 gaatcgagaa taaagcggat cgccgcaaac gggacttcat taacgaactt caggatctca
     8821 gccagcgtcg ttctggaaag caccgtcagc ggcgactctt gctcgccctc tgtcacacac
     8881 gcctgctggg taaacaccac accattgtgc gtctcaatat gcacaatgtt ggtatgcccg
     8941 ccgacgatgg tgacacacgc ccacttctca ccgttccaga ctttggcgcg tgagaagagg
     9001 atttcatcgc aaggttcctg gatcttaact gagactttcc ccgccgccag cagtgctttg
     9061 gcatcggaaa ttgcctgcgc agttgcgtct ttcagcactt ccagcccggc gttagcattt
     9121 ccacctaacg ctcccagcgc cgccgcaatt ggcagcccca ccattcctgt accaggaacg
     9181 gtgacaccca gaccgttctt catcagattt ggcgaaaccc aggcttctac acgttcaacc
     9241 ggaccttcca gttcagccgc agcaaccgcc gccgccagcg ccagtgaaat cggttcagta
     9301 caccccagcg ccggttttac ttcctcctga acggcgagga tgtaacgctg ccataacgga
     9361 tttaaagtcg aatcaaacat attaaaaacc ttaaaatttc aggtaaatca agaaaatgcc
     9421 aggaacggag aaacacaaag caacaaacca gtgacgataa tcaggtacag agacatccct
     9481 ttgtatttgt gcaatgccgg tactttgtaa accagccacg ccgggatcag gcaccctacc
     9541 atgccgaaaa tcgggctaca gatagaggtg aagctcaaca ccggggcgtt cagtacgatg
     9601 gcgctccagg ccagcaaaat ggcgaaaatc attatgccgc gctgaacgag attttcgtta
     9661 atcttctcgg caggtatctt gcgacgcagg atgttcatta cgatcccttg cgttgcttcg
     9721 cgaaagccta aatagacgcc aaagaatgca gtcattacag caaagatatt gagaataacg
     9781 ctgaccactt tcacccacgc tgcgccgtca ccgctaataa actgtgcggc aatcgccagc
     9841 gcggaaatat tctgctcgta ggcttttacc gcttcgtcat gtcccatcgc cagcgtgaat
     9901 gacacggcgt agaaaaagac ggtgacaaac aaaatgccaa acgcgatatt cattgcccgc
     9961 aatgctttat gccgcgccac ttcaattgat ttttcccgcg aacgataaga gatcaccatc
    10021 gggcttaacg tctggataaa cagaatcgac gtcagggtaa acggcagcgt aataatggcg
    10081 tttttcacca gcagccccag cggcggtagc gaaccgacgt tatacagatg ccacattccc
    10141 accatcgaca cgcccagcgc cgcgaccacc agcagcttgg tcagcaccat gccggtcgaa
    10201 attttgaata acaatttctc gccgcgtgag gagatcgcca ccagaatgca aatcagtacc
    10261 agaccataaa agggactgtc tgacagcaac ccttccgtca cgccgaaggt atgcaggtag
    10321 gaagcactat cgttggtgat ggcggtggaa tagacgaaca tccaaatcac cagcattacg
    10381 aaatagagtg cacctaacag gatgccccag tttttaccta aataaccgct gatgacgctc
    10441 gggtaatctt tacactccgg tgattctgcc agcgtattaa taaacaaccg ctgaaacaga
    10501 tacattgccg ggtaaccaat caccgatgag agcaaaaata cccacaatcc catcaaaccg
    10561 acctgcaccg ggagaaaaac aatccccgcg ccaatcgcca tcccgatact cataatcacc
    10621 cagccggtgt cagtactgtc gaatttgatc gcttctcgcc actcgctctc actcattcca
    10681 gcacgacccg ccggggtcga agcgtctgca atgacgcctt tattcgatgc aatttccata
    10741 atttctcgct caatattttg tagggcttat tatttttttc cgagccgcat caaggcgata
    10801 tgcggtctac gtttttgcag gcgagttatt agaataaaga aatgatacgc gtgagatcag
    10861 agaaaatctt cacaatcaag acttttaaat agcaattgtg ggtaataatt ttct
//
LOCUS       DADSYM010000150        10825 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2415
            SAMN08039579-rid11723173.denovo.151, whole genome shotgun sequence.
ACCESSION   DADSYM010000150 DADSYM010000000
VERSION     DADSYM010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039579
            Sequence Read Archive: SRR6362533
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10825)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10825)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980162.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 03:39:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,484
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 4,347
            CDSs (with protein)               :: 4,347
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 43 of 85
            Pseudo Genes (incomplete)         :: 37 of 85
            Pseudo Genes (internal stop)      :: 19 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10825
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039579-rid11723173.denovo.151"
                     /strain="LGE2415"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8302 N 95.9628 W"
                     /collection_date="2015-08-24"
     gene            67..828
                     /locus_tag="J1Y83_003642"
     CDS             67..828
                     /locus_tag="J1Y83_003642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HBA7631754.1"
                     /translation="MRICSDQPCIVLLTEKDVWIRVNGKEPISLKANHMALLNCENNI
                     IDVSSLNNTLVAHISHDIIKDYLRFLNKDLSQIPVWQRSATPILTLPCLTPDVFRVAA
                     QHSMMPAETESEKERTRALLFTVLSRFLDSKKFLSLMMYMLRNCVSDSVYQIIESDIH
                     KDWNLSMVASCLCLSPSLLKKKLKSENTSYSQIITTCRMRYAVNELMMDGKNISQVSQ
                     SCGYNSTSYFISVFKDFYGMTPLHYVSQHRERTVA"
     gene            965..2302
                     /gene="adiC"
                     /locus_tag="J1Y83_003643"
     CDS             965..2302
                     /gene="adiC"
                     /locus_tag="J1Y83_003643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine/agmatine antiporter"
                     /protein_id="HBA7631755.1"
                     /translation="MSSDADAHKVGLIPVTLMVSGNIMGSGVFLLPANLASTGGIAIY
                     GWLVTIIGALGLSMVYAKMSFLDPSPGGSYAYARRCFGPFLGYQTNVLYWLACWIGNI
                     AMVVIGVGYLSYFFPILKDPLVLTITCVVVLWIFVLLNIVGPKMITRVQAVATVLALI
                     PIVGIAVFGWFWFRGETYMAAWNVSGLGTFGAIQSTLNVTLWSFIGVESASVAAGVVK
                     NPKRNVPIATIGGVLIAAVCYVLSTTAIMGMIPNAALRVSASPFGDAARMALGDTAGA
                     IVSFCAAAGCLGSLGGWTLLAGQTAKAAADDGLFPPIFARVNKAGTPVAGLIIVGILM
                     TIFQLSSISPNATKEFGLVSSVSVIFTLVPYLYTCAALLLLGHGHFGKARPAYLAVTT
                     IAFLYCIWAVVGSGAKEVMWSFVTLMVITAMYALNYNRLHKNPYPLDAPISKD"
     gene            2406..4049
                     /gene="eptA"
                     /locus_tag="J1Y83_003644"
     CDS             2406..4049
                     /gene="eptA"
                     /locus_tag="J1Y83_003644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418538.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase EptA"
                     /protein_id="HBA7631756.1"
                     /translation="MLKRLLKRPSLNLFSWLLLATFYISVCLNIAFFKQVLQVLPLDS
                     LHNVLVFLSMPVVAFSVINIVLTLGSFLWLNRPLACLFILVGAAAQYFIMTYGIVIDR
                     SMITNIIDTTPAESYALMTPRMLLTLGLSGVLAAIIACWIKIKPTTSRLRSVLFRGAN
                     ILISVLLILLVAALFYKDYASLFRNNKELVKSLSPSNSIVASWSWYSHQRSANLPLIR
                     IGEDAHRNPLMQNGKRKNLTILIVGETSRAENFSLNGYPRETNPRLAKDNVVYFPNTA
                     SCGTATAVSVPCMFSDMPREHYKEELAQHQEGVLDIIQRAGINVLWNDNDGGCKGVCD
                     RVPHQNVTALNLPGQCINGECYDEVLFHGLEDYINNLQGDGVIVLHTIGSHGPTYYNR
                     YPPQFRKFTPTCDTNEIQTCTQEQLVNTYDNTLVYVDYIVDKAINLLKEHQDKFTTSL
                     VYLSDHGESLGENGIYLHGLPYAIAPDSQKQVPMLLWLSEDYQKRYQVDQNCLQKQAQ
                     TQHYSQDNLFSTLLGLTGVETKYYQAADDILQTCRRVSE"
     gene            4046..4714
                     /gene="basR"
                     /locus_tag="J1Y83_003645"
     CDS             4046..4714
                     /gene="basR"
                     /locus_tag="J1Y83_003645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BasR"
                     /protein_id="HBA7631757.1"
                     /translation="MKILIVEDDTLLLQGLILAAQTEGYACDGVSTARMAEQSLEAGH
                     YSLVVLDLGLPDEDGLHFLARIRQKKYTLPVLILTARDTLTDKIAGLDVGADDYLVKP
                     FALEELHARIRALLRRHNNQGESELNVGNLTLNMGRRQVWMSGEELILTPKEYALLSR
                     LMLKAGSPVHREILYNDIYNWDNEPSTNTLEVHIHNLRDKVGKARIRTVRGFGYMLVA
                     NEEN"
     gene            4715..5815
                     /gene="pmrB"
                     /locus_tag="J1Y83_003646"
     CDS             4715..5815
                     /gene="pmrB"
                     /locus_tag="J1Y83_003646"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     PmrB"
                     /protein_id="HBA7631758.1"
                     /translation="MNLMRFLRRPISLRQRLILTIGAILLVFELISVFWLWHESTEQI
                     QLFEQALRDNRNNDRHIMREIREAVASLIVPGVFMVSLTLFICYQAVRRITRPLAELQ
                     KELEARTADNLTPIAIHSATLEIDAVVSALNDLVSRLTSTLDNERLFTADVAHELRTP
                     LAGVRLHLELLAKTHHIDVAPLVARLDQMMESVSQLLQLARAGQSFSSGNYQHVKLLE
                     DVILPSYDELSTMLDQRQQTLLLPESAADITVQGDATLLRMLLRNLVENAHRYSPQGS
                     NIMIKLQEDGGAVMAVEDEGPGIDESKCGELSKAFVRMDSRYGGIGLGLSIVSRITQL
                     HHGQFFLQNRQETSGTRAWIRLKKDQYVANQI"
     gene            complement(5791..5880)
                     /gene="pmrR"
                     /locus_tag="J1Y83_003647"
     CDS             complement(5791..5880)
                     /gene="pmrR"
                     /locus_tag="J1Y83_003647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003826741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LpxT activity modulator PmrR"
                     /protein_id="HBA7631759.1"
                     /translation="MKNRVYESLTTVFSVLVVSSFLYIWFATY"
     gene            complement(5992..7494)
                     /gene="proP"
                     /locus_tag="J1Y83_003648"
     CDS             complement(5992..7494)
                     /gene="proP"
                     /locus_tag="J1Y83_003648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline transporter ProP"
                     /protein_id="HBA7631760.1"
                     /translation="MLKRKKVKPITLRDVTIIDDGKLRKAITAASLGNAMEWFDFGVY
                     GFVAYALGKVFFPGADPSVQMVAALATFSVPFLIRPLGGLFFGMLGDKYGRQKILAIT
                     IVIMSISTFCIGLIPSYDTIGIWAPILLLICKMAQGFSVGGEYTGASIFVAEYSPDRK
                     RGFMGSWLDFGSIAGFVLGAGVVVLISTIVGEENFLDWGWRIPFFIALPLGIIGLYLR
                     HALEETPAFQQHVDKLEQGDREGLQDGPKVSFKEIATKHWRSLLTCIGLVIATNVTYY
                     MLLTYMPSYLSHNLHYSEDHGVLIIIAIMIGMLFVQPVMGLLSDRFGRRPFVLLGSVA
                     LFVLAIPAFILINSNVIGLIFAGLLMLAVILNCFTGVMASTLPAMFPTHIRYSALAAA
                     FNISVLVAGLTPTLAAWLVESSQNLMMPAYYLMVVAVVGLITGVTMKETANRPLKGAT
                     PAASDIQEAKEILVEHYDNIEQKIDDIDHEIADLQAKRTRLVQQHPRIDE"
     gene            complement(7758..8636)
                     /locus_tag="J1Y83_003649"
     CDS             complement(7758..8636)
                     /locus_tag="J1Y83_003649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA7631761.1"
                     /translation="MTKTLLDGPGRVLESVHPRFLVDLAQGDDARLPQAHQQQFRERL
                     MQELLARVQLQTWTNGGMLNAPLSLRLTLVEKLASMLDPGHLALTQIAQHLALLQKMD
                     HRQHSAFPELPQQIVALYEWFSARCRWKEKALTQRGLLVQAGEQSEQIFTRWRAGAYN
                     AWSLPGRCFIVLEELRWGAFGDACRLGSPQAVALLLGDLRVKATQHLAESINAAPTTR
                     HYYHQWFASSTVSTGGDHADFLSWLGKWSTADKQPVCWSVTQRWRTVALGMPRLCSAQ
                     RLAGAMVEEIFSVNLV"
     gene            complement(8633..>10825)
                     /gene="crfC"
                     /locus_tag="J1Y83_003650"
     CDS             complement(8633..>10825)
                     /gene="crfC"
                     /locus_tag="J1Y83_003650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="clamp-binding protein CrfC"
                     /protein_id="HBA7631762.1"
                     /translation="AERLLQLSRQQLQLLEKMPLSVPGDDAPQLALPWSQPNIAERHA
                     MLNNELRKISRLEMVLAIVGTMKAGKSTTINAIVGTEVLPNRNRPMTALPTLIRHTPG
                     QKEPVLHFSHVAPIDCLIQKLQQRLRDCDIKHLTDVLEIDKDMRALMQRIENGVAFEK
                     YYLGAQPIFHCLKSLNDLVRLAKALDVDFPFSAYAAIEHIPVIEVEFVHLAGLESYPG
                     QLTLLDTPGPNEAGQPHLQKMLNQQLARASAVLAVLDYTQLKSISDEEVREAILAVGQ
                     SVPLYVLVNKFDQQDRNSDDADQVRALISGTLMKGCITPQQIFPVSSMWGYLANRARH
                     ELANNGKLPAPEQQRWVEDFAHAALGRRWRHADLADLEHIRHAADQLWEDSLFAQPIQ
                     ALLHAAYANASLYALRSAAHKLLNYAQQAREYLDFRAHGLNVACEQLRQNIHQVEESL
                     QLLQLNQAQVSGEIKHEIELALTCANHFLRQQQDAVNAQLAALFQDDSGSLSEMRTRC
                     ETLLQTAQNTISRDFMLRFAELESTLCRVLTDVIRPIEQQVKMELSESGFRPGFHFPV
                     FHGAVPHFNTRQLFSEVISRQAATDEQSTRLGVVRETFSRWLNQPDWGRGNEKSPTET
                     VDYSALQRALSAEVDLYCQQMAKVLAEQVDESVTAGMNTFFAEFASCLTELQTCLRES
                     LALRQQNESVVRLMQQQLQQTVMTHGWIYTDAQLLRDDIQTLFTAERY"
BASE COUNT         2721 a         2858 c         2648 g         2598 t
ORIGIN      
        1 accatttccc ggtatcgctt cgctttagcg agaactggtc ttttattcgc actcaggagt
       61 acatgtatga ggatttgcag cgaccaacct tgtattgttt tattgactga aaaagatgtc
      121 tggataaggg tgaatgggaa agaacctatt agccttaaag ctaaccacat ggcgttatta
      181 aattgtgaaa ataatattat cgacgtctcc tctcttaata acactttggt tgctcatatt
      241 agtcacgaca tcatcaaaga ttacctccgg tttctgaata aagatctctc gcaaatacca
      301 gtatggcaac gtagcgctac gcccatcctc accctgccat gcctgacgcc agacgtcttt
      361 cgcgttgccg cgcagcatag catgatgccc gctgaaactg agtcagaaaa ggaacgaaca
      421 cgtgcattat tattcactgt gctatcccgt tttctcgaca gtaaaaaatt cctttcacta
      481 atgatgtata tgttacgtaa ttgtgtaagt gacagcgttt atcaaattat tgaaagcgat
      541 atccacaaag actggaatct tagtatggta gccagttgtt tatgtcttag cccaagtctg
      601 ttaaagaaaa agctgaaaag cgaaaacacc agttatagcc aaataatcac cacctgccgc
      661 atgcgttatg ccgtaaatga attaatgatg gacggtaaaa atatctctca ggtatcacag
      721 tcctgcggct acaacagtac gtcgtacttt atttctgtct ttaaagactt ctacggtatg
      781 acgccgctgc attatgttag tcagcacaga gaacgcactg tcgcctgatt tttaacctta
      841 acgaagagct atattaataa cggcatcagc gataacccgg ccgataataa ttcaactatc
      901 gaatgcaggc gtatgatatg acgtaattta ttgtcacgaa gctcgccttc gcaggagttt
      961 aattatgtct tcggatgctg atgctcacaa agtgggctta atccccgtca ccctgatggt
     1021 gtcggggaat attatggggt caggtgtttt tctgttacct gcaaacctgg cctccactgg
     1081 cgggattgcc atttatggat ggttggtgac gattatcggt gcgctggggc tctcgatggt
     1141 atacgccaaa atgtcgttcc tcgacccaag tcctggtggt tcttacgctt acgcccgccg
     1201 ctgctttggc ccgtttctcg gttatcaaac caacgtcctc tactggctgg cctgctggat
     1261 cggcaatatc gccatggtgg tcattggcgt aggatattta agttacttct tcccgattct
     1321 gaaagaccca ttggtattaa ccatcacctg cgtcgtggtg ctgtggatct tcgtcctgct
     1381 gaacattgtc ggtccgaaaa tgatcacccg cgtacaggcc gttgctaccg tgctggcgct
     1441 gatccccatc gtcgggattg ccgtatttgg ctggttctgg ttccgtggtg aaacctatat
     1501 ggcggcatgg aacgtcagcg gcctgggcac cttcggtgcg attcaaagta cccttaacgt
     1561 tacgctgtgg tcgttcatcg gtgtggaaag tgcctccgtt gccgcaggtg tggtgaaaaa
     1621 cccgaaacgc aacgtcccta tcgccaccat tggcggggta ttgattgctg ccgtttgcta
     1681 tgtactttct accaccgcga ttatggggat gatccctaat gccgcactgc gcgtttctgc
     1741 ttcgccattc ggtgatgccg cacggatggc gttgggtgac accgccgggg ccattgtttc
     1801 cttctgcgca gctgcgggtt gcttaggttc actgggcggc tggacgttgc tggcgggtca
     1861 aacggcgaaa gccgctgccg atgacggact gttcccaccg atttttgccc gtgtaaataa
     1921 agcgggcacg ccagtggcgg ggctgattat cgtcggtatt ttgatgacca tcttccagct
     1981 cagtagcatt tcgccaaacg cgactaaaga attcggtctg gtttcttccg tgtcggtcat
     2041 ctttacactg gtgccatatc tttacacctg tgcggcgtta ctactgctcg gccacggtca
     2101 ctttggtaaa gcacgcccgg catacctggc agttactacc attgccttcc tctactgcat
     2161 ctgggccgta gtagggtccg gcgcgaaaga ggttatgtgg tcatttgtca ctctgatggt
     2221 aatcaccgcc atgtatgcgc tgaattacaa ccggctgcat aaaaacccgt atcccttaga
     2281 tgcaccaata agcaaagatt aattcccctt aatcaagcaa acataaaagc caaccttaag
     2341 aacttaaggt tggcttaatt ttgctttgcg agcatatgcg cactttgttc gatggaaaca
     2401 ccgtgatgtt gaagcgccta ctaaaaagac cctctttaaa tttattctcc tggttattac
     2461 ttgctacttt ttatatctca gtctgcctga atatcgcctt ttttaaacag gtgctacagg
     2521 tgttaccgct ggattcacta cataacgtgc tggttttctt gtcgatgccg gtcgtcgcat
     2581 tcagcgtgat taatattgtc ctgacactag gctctttctt atggcttaat cgtccactcg
     2641 cctgcctgtt tattctggtc ggtgcggctg cacaatattt catcatgacc tatggcatcg
     2701 tcatcgaccg ttcaatgatc accaatatca ttgatactac accggcggaa agttatgcgt
     2761 tgatgacgcc acgaatgtta ttaacgctgg ggttaagtgg cgtgttagcc gcgattattg
     2821 cctgctggat caaaatcaaa cccaccacct cacgcctgcg cagcgttctt ttccgcgggg
     2881 ccaacatcct gatttctgtg ctgctgattc tgctggttgc cgcactgttc tacaaagact
     2941 atgcctcgct gtttcgcaac aacaaagagc tggtgaaatc cttaagcccg tccaacagca
     3001 ttgttgctag ctggtcgtgg tactctcacc agcggtcagc caacctgcca ctgatacgca
     3061 ttggtgaaga tgctcaccgc aacccattaa tgcaaaatgg gaaacgcaaa aacctgacca
     3121 ttttgatcgt cggagaaacg tcgcgcgcag agaatttctc gctgaatggt tatccacgcg
     3181 aaaccaatcc tcgcctggca aaagataacg tggtctattt ccctaatacc gcatcttgtg
     3241 gcacagcaac agccgtctca gtgccgtgca tgttctcgga tatgccgcgt gagcactaca
     3301 aagaagagct ggcacagcac caggaaggcg tgctggatat cattcagcga gcgggcatca
     3361 acgtgctgtg gaatgacaac gatggcggct gtaaaggcgt ttgcgatcgc gtacctcacc
     3421 agaacgtcac cgcgctgaac ctgcctggtc agtgcatcaa cggcgaatgc tatgatgaag
     3481 tgctgttcca cgggctggaa gattacatca ataacctgca gggtgatggc gtgattgtct
     3541 tacacaccat cggcagccac ggcccgacct attacaaccg ctatccgccg cagttcagga
     3601 aatttacccc aacctgcgac actaacgaga tccagacctg tacccaagag caactggtga
     3661 acacttacga caacacgctg gtttacgtcg actatattgt tgataaagcg attaatctgc
     3721 tgaaagaaca tcaggataaa tttaccacca gcctggttta tctttctgac cacggtgaat
     3781 cgttaggtga aaatggcatc tatctgcacg gtctgcctta tgccatcgcc ccggatagcc
     3841 aaaaacaggt gccgatgctg ctgtggctat cggaggatta tcaaaaacgg tatcaggttg
     3901 accagaactg cctgcaaaaa caggcgcaaa cgcaacacta ttcacaagac aatttattct
     3961 caaccttatt gggcctgact ggcgttgaga cgaagtatta ccaggctgcg gatgatattc
     4021 tgcaaacttg caggagagtg agtgaatgaa aattctgatt gttgaagacg atacgctgtt
     4081 attgcaggga ttgattctgg cggcgcaaac cgaaggctac gcgtgcgatg gcgtgagcac
     4141 cgcgcggatg gcggaacaaa gccttgaggc cggtcattac agcctggtgg tactggattt
     4201 aggcttaccc gatgaagatg ggctgcattt tcttgcccgt atccggcaga aaaaatacac
     4261 tctgccggtg ttaattctca ccgcccgcga tacccttacc gacaaaatcg ccgggctgga
     4321 tgtcggggcc gacgattatc tggtgaagcc ttttgcgctg gaagagttgc acgcccgtat
     4381 ccgcgccctg ctacgacgcc ataataatca gggcgaaagt gagctgaacg ttggcaacct
     4441 gacgctgaat atgggtcgcc gccaggtatg gatgagcgga gaagaactga ttctgacgcc
     4501 caaagaatat gctctgctgt cgcgcctgat gcttaaagca ggcagcccgg tgcatcggga
     4561 aattctctac aacgatatct acaactggga caacgagccc tcgaccaaca ccctggaagt
     4621 gcatatccat aacctgcgcg acaaagtggg caaagcccgt atccgcaccg tgcgcggatt
     4681 tggctatatg ctggtcgcaa atgaggaaaa ctaattgaat ctgatgcgtt ttctgcgccg
     4741 accaatatcg ctgcgccaac ggctgatatt gaccatcggg gccattttgt tggtgtttga
     4801 gctgatcagc gtcttctggc tatggcatga aagtaccgag cagattcagc tgtttgagca
     4861 ggcgctacgc gacaatcgca acaacgatcg ccacatcatg cgtgagatcc gcgaggcggt
     4921 cgccagcctg attgtccccg gcgtctttat ggttagcctg acgctattta tctgctatca
     4981 ggcggtacgc cgcatcaccc gaccgctggc ggagctgcaa aaagagctgg aagcgcgcac
     5041 cgccgacaac ttaacgccca ttgccattca tagcgccacc ctcgaaatcg atgcggtggt
     5101 ttcggcatta aacgatctgg tcagtcgcct gaccagcacg ctggataacg aaaggttgtt
     5161 taccgctgac gtcgcgcacg aactgcgaac gccactggcg ggggtgcgtt tgcatctgga
     5221 actgctggcg aaaacgcatc acattgatgt agcaccgtta gtggcacgac ttgatcagat
     5281 gatggagagc gtctcccagc tgctgcaact ggcgcgtgcc ggacagtcat tttcttccgg
     5341 taattatcaa catgtaaaac tgctggaaga tgtcattctc ccctcgtatg acgaacttag
     5401 taccatgctc gaccagcgcc agcaaaccct gctattgcca gagagcgccg ccgacatcac
     5461 cgttcagggt gatgcaaccc tgctgcggat gttattgcga aacctggtag aaaacgccca
     5521 tcgttatagc ccacaaggca gcaacattat gattaagctg caagaagatg gcggagcggt
     5581 catggccgtt gaagatgaag gaccgggtat tgatgagagt aaatgcgggg agttgagtaa
     5641 agcgtttgta cgaatggaca gccgttatgg cgggattggt ctggggttaa gtattgtcag
     5701 ccgcattaca cagttgcatc acgggcagtt tttcctgcaa aaccggcaag agacttccgg
     5761 cacgcgggcc tggatacggc tgaaaaaaga tcagtacgtg gcaaaccaga tataaagaaa
     5821 gctgctgacc accagcacgc tgaacacggt agttaaactt tcataaacac ggtttttcat
     5881 tgcactctcc tccgttaacc tggaggagag tatgcgcgtc agagattaag tgaaccttaa
     5941 gagttcaatt gacctgatta gacagcgtca catcaggcca tccgtttcag cttattcatc
     6001 aattcgcgga tgttgctgca ccaggcgggt acgtttcgcc tgcaaatcgg caatctcgtg
     6061 gtcaatatca tcgattttct gctcgatatt atcgtaatgc tcgacgagaa tttccttcgc
     6121 ttcctgtata tctgacgccg ctggtgtcgc acctttcaac ggtcgattcg ccgtctcttt
     6181 catggttacg ccggtgatta aaccaaccac cgccactacc atcaggtaat aggcaggcat
     6241 catcagattc tgcgagcttt cgaccagcca ggccgccagc gttggcgtca gaccggcaac
     6301 cagcaccgaa atattaaatg ccgccgccag cgcgctgtaa cggatatgcg tcgggaacat
     6361 cgcaggcaag gtagaagcca taacgcccgt aaagcagtta aggatcaccg ccagcatcaa
     6421 taagccggca aaaatcaggc cgatgacgtt actgttaatc agaataaacg ccgggatcgc
     6481 caacacaaac agtgcaacac taccaagaag cacaaacgga cgacggccaa aacggtcact
     6541 cagcaagccc atcaccggtt gaacaaacag cataccgatc ataatggcga taataatcag
     6601 caccccgtgg tcttcggagt aatgcaggtt atgcgacaaa taactcggca tataggtcag
     6661 caacatgtag taagtcacgt tggtagcaat taccagacca atacatgtca acaggctgcg
     6721 ccagtgtttg gtggcaatct ctttaaacga gactttcggg ccatcctgca aaccttcgcg
     6781 gtcgccctgt tccagtttat cgacatgctg ctggaacgcc ggagtctctt ccagcgcatg
     6841 gcgcaggtaa agcccgataa tccctaacgg cagagcgata aagaacggaa tacgccagcc
     6901 ccaatcgagg aagttctctt cgccgacaat ggtcgaaatt aacaccacta cgccagctcc
     6961 cagcacaaac ccagcaatag aaccaaagtc cagccagctg cccataaagc cacgtttacg
     7021 gtcaggggag tattccgcaa caaatattga cgccccggta tattcaccgc cgaccgagaa
     7081 accttgtgcc atcttacaga tcaacagcag aatcggtgcc caaataccaa tcgtgtcgta
     7141 ggacggtatt aagccaatac agaacgtact gatcgacata atcacaatag tgatagcgag
     7201 gatcttctgg cgaccatatt tatcgcccaa cataccaaag aagagcccgc caagcggtcg
     7261 aatcagaaag ggaacggaga aagtggcgag tgcagcaacc atctgcacgc tggggtcagc
     7321 ccccgggaaa aaaactttac ctaatgcgta agcaacaaaa ccataaacac caaaatcgaa
     7381 ccattccatt gcattaccca gtgatgctgc ggtaatggct ttacgcagtt taccgtcatc
     7441 aataatggtg acatcacgaa gggtaatcgg ttttactttt ttccttttca gcatagcttt
     7501 cctcgcagag ttggcccatt tccgcaaacg ccagaaaaat cagcgcgacg gccgcaaaag
     7561 gggagcggga attgcaggat gcaatctctg taacaagcgt agcgggtttg cttacaccct
     7621 ccggttaagg aatgtacaat caaaccagtt gacgcctgaa ttgggcagtt aatgaccttt
     7681 ttaccctacc agtgtttaaa tttgtgatca acttcacaca tgggtttcgt ttaggactca
     7741 ttggtgtaac tgattattta caccaaattt acagagaaga tttcctcgac cattgccccc
     7801 gccagacgct gcgctgaaca gagtcgtggc atccccagtg cgacagttcg ccagcgttgg
     7861 gtcactgacc agcaaacggg ttgtttatct gccgtagacc actttcccag ccaacttaaa
     7921 aaatcagcat gatccccgcc cgtcgaaacg gtcgaagagg caaaccactg atggtaatag
     7981 tgacgcgtgg tcggggccgc attgatactc tctgccaaat gttgtgtcgc tttcacgcgc
     8041 aaatcaccca gcaacaacgc caccgcttgc gggcttccca gacggcaggc atcgccaaat
     8101 gcaccccagc gcaactcctc cagaacgata aaacagcgcc caggcaacga ccaggcgtta
     8161 tacgccccag cacgccagcg ggtaaaaatt tgctcgctct gttcacccgc ctgcaccaat
     8221 aggcctcgtt gcgttaacgc cttttccttc cagcgacaac gggctgaaaa ccactcatac
     8281 aaggcgacaa tttgctgggg gagctccggg aaagcagagt gctggcggtg atccattttt
     8341 tgcagcagcg ccagatgctg cgcgatctgc gtcagcgcca gatgaccggg atccagcatc
     8401 gacgccagtt tctccaccaa tgtcagacgc agactaagcg gcgcatttaa catgccgccg
     8461 ttcgtccatg tctgaagctg cacgcgcgca agaagttcct gcatcagtcg ttcacgaaac
     8521 tgctgctgat gggcttgtgg aaggcgggca tcatcgccct gcgccagatc cactaaaaag
     8581 cgcggatgaa ccgactccag cacgcgaccg gggccgtcca gtaacgtctt ggtcaatatc
     8641 gttctgccgt gaaaagtgtt tgaatatcat cgcgtaacag ctgagcgtcg gtgtaaatcc
     8701 agccgtgagt catcacagtc tgctgcaatt gctgttgcat cagcctgacc accgattcat
     8761 tttgttgacg cagagccaga ctttcgcgta aacacgtctg taattccgtc aaacatgaag
     8821 cgaactcagc gaaaaaagta ttcatgcctg ccgtaacaga ttcatcgacc tgctctgcca
     8881 gaactttagc catttgttgg caataaagat cgacttctgc gcttaatgct cgttgcaacg
     8941 cactgtaatc aaccgtctct gtcggggatt tctcatttcc ccgtccccag tcgggctgat
     9001 tcaaccagcg cgaaaaagtc tcacgcacaa cgcctaaacg cgtgctctgc tcgtccgttg
     9061 ctgcctggcg cgaaatgact tcactgaaca gctggcgagt gttgaagtgg ggaactgcgc
     9121 cgtgaaaaac aggaaaatga aacccaggac gaaaccctga ctcactcaat tccattttga
     9181 cttgttgctc aatggggcga ataacatcgg ttaacacccg gcaaagagtg gattcaagct
     9241 cggcaaaacg cagcataaag tcgcgactga tggtgttctg cgccgtctgt aacagtgtct
     9301 cacagcgggt acgcatctcg cttaatgacc ccgaatcatc ctgaaacaac gcggctaact
     9361 gcgcattcac cgcatcttgc tgttgacgca gaaagtggtt ggcgcaggtc agggccaact
     9421 cgatttcatg tttaatctcg ccgctcacct gcgcctgatt gagttgcaat agctgcaaac
     9481 tttcttcgac ctgatggata ttttgccgca attgttcaca agcgacgttt aacccgtgcg
     9541 cacgaaaatc caggtattcc cgcgcctgct gcgcgtaatt caacagttta tgcgcggcag
     9601 atcgcagagc atacaacgag gcgttagcgt aagcggcatg aagcaacgcc tgaattggct
     9661 gggcgaacag cgaatcttcc cacaactgat cggcagcatg gcgaatatgt tcgaggtccg
     9721 ccagatcggc atgacgccag cgcctgccga gcgcggcatg ggcaaaatct tccacccagc
     9781 gttgttgctc tggcgcgggt aacttaccgt tgttggctaa ctcatggcgc gcccgattcg
     9841 ccaggtagcc ccacatcgac gacaccggaa atatctgctg tggcgtaata cagcctttca
     9901 tcagcgttcc ggaaatcagt gcccgcacct ggtcggcgtc gtcactgtta cgatcctgtt
     9961 gatcgaactt attgaccagc acatacaacg gcaccgattg ccccaccgcc aaaattgcct
    10021 cacggacctc ttcatcggag atcgatttca gttgcgtata atccagcacc gccagtaccg
    10081 ccgaggcgcg tgccagctgc tggttaagca ttttttgcag atgcggttgc ccggcttcat
    10141 ttggcccggg ggtatccagt aacgtcaact gaccgggata actctccagc cccgccagat
    10201 ggacaaactc cacttcaatc acgggaatat gctcaatggc ggcgtaagca gaaaaaggaa
    10261 aatcgacgtc cagcgccttc gccagtcgca ccaaatcatt caaacttttc agacaatgaa
    10321 aaataggctg ggcacccaga taatattttt cgaaagcgac gccattttcg atccgctgca
    10381 taagcgcacg catatcttta tctatttcca gcacatcggt cagatgctta atatcgcaat
    10441 cacgcaggcg ctgttgtaat ttttgaatta aacaatcgat tggcgcgaca tgtgaaaaat
    10501 gcagtaccgg ttctttttgc ccgggcgtat ggcgaataag cgttggcagc gcagtcattg
    10561 ggcgattgcg attaggcaga acctccgtac caacaatggc attaatggtg gttgatttcc
    10621 ctgctttcat agtaccgaca atcgccagca ccatttccag tcgggaaatt ttacgcaact
    10681 cattattcag catcgcgtga cgttcggcga tattaggctg actccagggt aaagccagtt
    10741 gtggcgcatc gtctccgggt acagagagag gcattttttc cagtaactgc aactgttggc
    10801 gagaaagctg taacaggcgt tcagc
//
LOCUS       DADSYM010000151        10740 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2415
            SAMN08039579-rid11723173.denovo.152, whole genome shotgun sequence.
ACCESSION   DADSYM010000151 DADSYM010000000
VERSION     DADSYM010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039579
            Sequence Read Archive: SRR6362533
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10740)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10740)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980162.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 03:39:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,484
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 4,347
            CDSs (with protein)               :: 4,347
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 43 of 85
            Pseudo Genes (incomplete)         :: 37 of 85
            Pseudo Genes (internal stop)      :: 19 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10740
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039579-rid11723173.denovo.152"
                     /strain="LGE2415"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8302 N 95.9628 W"
                     /collection_date="2015-08-24"
     gene            151..1104
                     /locus_tag="J1Y83_003651"
     CDS             151..1104
                     /locus_tag="J1Y83_003651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001571919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HBA7631763.1"
                     /translation="MAKKAGQIVGRITAQIDTLHRERYGEDTGHFGMIDAIDDPQVFA
                     ALFGAAEAWLKSQGASKISGPFSLNINQESGLLIEGFDTPPCAMMPHGKPWYAAHIEQ
                     LGYHKGIDLLAWWMQRTDLTFSPALKKLMDQVRKKVTIHCINRQRFAEEMQILREIFN
                     SGWQHNWGFVPFTEHEFATMGDQLKYLVPDDMIYIAEIDSAPCAFIVGLPNINEAIAD
                     LNGSLFPFGWAKLLWRLKVSGVRTARVPLMGVRDEYQFSRIGPVIALLLIEALRDPFA
                     RRKIDALEMSWILETNTGMNNMLERIGAEPYKRYRLYEKQI"
     gene            complement(1111..1875)
                     /gene="glcC"
                     /locus_tag="J1Y83_003652"
     CDS             complement(1111..1875)
                     /gene="glcC"
                     /locus_tag="J1Y83_003652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001305094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GlcC"
                     /protein_id="HBA7631764.1"
                     /translation="MKDERRPICEVVAESIERLIIDGVLKVGQPLPSERRLCEKLGFS
                     RSALREGLTVLRGRGIIETAQGRDSRVARLNRVQDTSPLIHLFSTQPRTLYDLLDVRA
                     LLEGESARLAATLGTQADFVVITRCYEKMLAASENNKEISLIEHAQLDHAFHLAICQA
                     SHNQVLVFTLQSLTDLMFNSVFASVNNLYHRPQQKKQIDRQHARIYNAVLQRLPHVAQ
                     RAARDHVRTVKKNLHDIELEGHHLIRSAVPLEMKQG"
     gene            2126..3625
                     /gene="glcD"
                     /locus_tag="J1Y83_003653"
     CDS             2126..3625
                     /gene="glcD"
                     /locus_tag="J1Y83_003653"
                     /EC_number="1.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcD"
                     /protein_id="HBA7631765.1"
                     /translation="MSILYEERLDGALPEVDRTSVLMALREHVPRLEILHTDEEIIPY
                     ECDGLSAYRTRPLLVVLPKQMEQVTAILAVCHRLRVPVVTRGAGTGLSGGALPLEKGV
                     LLVMARFKEILDINPIGRRARVQPGVRNLAISQAVAPHNLYYAPDPSSQIACSIGGNV
                     AENAGGVHCLKYGLTVHNLLKIEVQTLDGEALTLGSDALDSPGFDLLALFTGSEGMLG
                     VTTEVTVKLLPKPPVARVLLASFDSVEKAGLAVGDIIANGIIPGGLEMMDNLSIRAAE
                     DFIHAGYPVDAEAILLCELDGVESDVQEDCERVNDILLNAGATDVRLAQDEAERVRFW
                     AGRKNAFPAVGRISPDYYCMDGTIPRRALPGVLEGIARLSQQYDLRVANVFHAGDGNM
                     HPLILFDANEPGEFARAEELGGKILELCVEVGGSISGEHGIGREKINQMCAQFNSDEI
                     TTFHAVKAAFDPDGLLNPGKNIPTLHRCAEFGAMHVHHGHLPFPELERF"
     gene            3625..4677
                     /gene="glcE"
                     /locus_tag="J1Y83_003654"
     CDS             3625..4677
                     /gene="glcE"
                     /locus_tag="J1Y83_003654"
                     /EC_number="1.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcE"
                     /protein_id="HBA7631766.1"
                     /translation="MLRECDYSQALLEQVNQAISDKTPLVIQGSNSKAFLGRPVTGQT
                     LDVRCHRGIVNYDPTELVITARAGTPLVAIEAALESAGQMLPCEPPHYGEEATWGGMV
                     ACGLAGPRRPWSGSVRDFVLGTRIITGTGKHLRFGGEVMKNVAGYDLSRLMAGSYGCL
                     GVLTEISMKVLPRPRASLSLRREISLQEAMNEIAQWQLQPLPVSGLCYFDNALWIRLE
                     GGEGSVKAARELLGGEEVAGQFWQQLREQQLPFFSLPGTLWRISLPSDAPMMDLPGEQ
                     LIDWGGALRWLKSTADDNQIHRIARNAGGHATRFSAGNGGFAPLPAPLFRYHQQLKQQ
                     LDPCGVFNPGRMYAEL"
     gene            4688..5911
                     /gene="glcF"
                     /locus_tag="J1Y83_003655"
     CDS             4688..5911
                     /gene="glcF"
                     /locus_tag="J1Y83_003655"
                     /EC_number="1.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcF"
                     /protein_id="HBA7631767.1"
                     /translation="MQTRLTEEMRQNARALEADSILRACVHCGFCTATCPTYQLLGDE
                     LDGPRGRIYLIKQVLEGNEVTLKTQEHLDRCLTCRNCETTCPSGVRYHNLLDIGRDIV
                     EQKVKRPLPERMLREGLRQVVPRPAVFRALTQVGLVLRPFLPEQVRAKLPAETVKAKP
                     RPPLRHKRRVLMLEGCAQPTLSPNTNAATARVLDRLGISVMPANEAGCCGAVDYHLNA
                     QEKGLARARNNIDAWWPAIEAGAEAILQTASGCGAFVKEYGQMLKNDALYADKARQVS
                     ELAVDLVELLREEPLEKLAIRGDKKLAFHCPCTLQHAQKLNGEVEKVLLRLGFTLMDV
                     PDSHLCCGSAGTYALTHPDLARQLRDNKMNALESGKPEMIVTANIGCQTHLASAGRTS
                     VRHWIEIVEQALEKE"
     gene            5916..6320
                     /locus_tag="J1Y83_003656"
     CDS             5916..6320
                     /locus_tag="J1Y83_003656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein GlcG"
                     /protein_id="HBA7631768.1"
                     /translation="MKTKVILSQQMASAIIAAGQEEAQKNNWSVSIAVADDGGHLLAL
                     SRMDDCAPIAAYISQEKARTAALGRRETKGYEEMVNNGRTAFVTAPLLTSLEGGVPVV
                     VDGQIIGAVGVSGLTGAQDAQVAKAAAAVLAK"
     gene            6342..8513
                     /gene="glcB"
                     /locus_tag="J1Y83_003657"
     CDS             6342..8513
                     /gene="glcB"
                     /locus_tag="J1Y83_003657"
                     /EC_number="2.3.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase G"
                     /protein_id="HBA7631769.1"
                     /translation="MSQTITQGRLRIDANFKRFVDEEVLPGVELDAAAFWHNVDEIVH
                     DLAPENRQLLAERDRIQAALDEWHRSNPGPVKDKAAYKSFLRELGYLVPQPDHVTVET
                     TGIDSEITSQAGPQLVVPAMNARYALNAANARWGSLYDALYGSDIIPQEGAMVSGYDP
                     QRGEQVIAWVRRFLDESLPLENGSYQDVVAFKVVDKQLRIQLKNGKETTLRTPAQFVG
                     YRGDTTALTCILLKNNGLHIELQIDANGRIGKDDPAHINDVIVEAAISTILDCEDSVA
                     AVDAEDKILLYRNLLGLMQGTLQEKMEKNGRQIVRKLNDDRQYTAADGSEISLHGRSL
                     LFIRNVGHLMTIPVIWDSEGNEIPEGILDGVMTGAIALYDLKVQKNSRTGSVYIVKPK
                     MHGPQEVAFANKLFSRVETMLGMAPNTLKMGIMDEERRTSLNLRSCIAQARNRVAFIN
                     TGFLDRTGDEMHSVMEAGPMLRKNQMKSTPWIKAYERNNVLSGLFCGLRGKAQIGKGM
                     WAMPDLMADMYSQKGDQLRAGANTAWVPSPTAATLHALHYHQTNVQSVQANIAQTEFN
                     AEFEPLLDDLLTIPVAENANWSAQEIQQELDNNVQGILGYVVRWVEQGIGCSKVPDIH
                     NVALMEDRATLRISSQHIANWLRHGILTKDQVQASLENMAKVVDQQNAGDPAYRPMAG
                     NFANSCAFKAACDLIFLGVKQPNGYTEPLLHAWRLREKENH"
     gene            8869..10551
                     /gene="glcA"
                     /locus_tag="J1Y83_003658"
     CDS             8869..10551
                     /gene="glcA"
                     /locus_tag="J1Y83_003658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate permease GlcA"
                     /protein_id="HBA7631770.1"
                     /translation="MVTWTQMYMPMGGLGLSALVALIPIIFFFVALAVLRLKGHVAGA
                     ITLILSILIAIFAFKMPIDMAFAAAGYGFIYGLWPIAWIIVAAVFLYKLTVASGQFDI
                     IRSSVISITDDQRLQVLLIGFSFGALLEGAAGFGAPVAITGALLVGLGFKPLYAAGLC
                     LIANTAPVAFGALGVPILVAGQVTGIDPFDIGAMAGRQLPFLSVLVPFWLVAMMDGWK
                     GVKETWPAALVAGGSFAVTQFFTSNYIGPELPDITSALVSIVSLSLFLKVWRPKNTET
                     AISMGQSAGAMVVNKPSSGGPVPSEYRLGQIIRAWSPFLILTVLVTIWTMKPFKALFA
                     PGGAFYSLVINFQIPHLHQQVLKAAPIVAQPTPMDAVFKFDPLSAGGTAIFIAAIISI
                     FILGVGIKKGIGVFAETLISLKWPILSIGMVLAFAFVTNYSGMSTTLALVLAGTGVMF
                     PFFSPFLGWLGVFLTGSDTSSNALFGSLQSTTAQQINVSDTLLVAANTSGGVTGKMIS
                     PQSIAVACAATGMVGRESELFRYTVKHSLIFASVIGIITLLQAYVFTGMLVS"
BASE COUNT         2489 a         2695 c         3036 g         2520 t
ORIGIN      
        1 attaataaaa aagaccttaa ggcatttatc gcttttccgt catcacttta tcccgatgat
       61 ccaaactgga tacccccttt attcattgaa cgcagcgagc atttgtctgc gaagaatcag
      121 gggacggacc atatcatctg gcaggcgtgg gtggcaaaaa aagcggggca gatagttggg
      181 cgtattaccg cgcaaataga taccttgcac cgtgagcgtt acggtgaaga taccggtcat
      241 ttcggaatga ttgatgccat tgatgatccg caggtttttg ccgcgttgtt tggcgcagcg
      301 gaggcgtggt tgaagtcaca aggtgcaagt aagatcagcg ggcctttcag cctgaatatc
      361 aatcaggaaa gcggattact gattgaaggt tttgacacac ctccctgtgc gatgatgcca
      421 cacggcaaac cgtggtatgc cgcgcatatt gaacaactgg gttatcacaa aggcattgat
      481 ttactggcgt ggtggatgca gcgaaccgat ctcactttct ctccggcgct aaaaaaactg
      541 atggatcagg tgcgcaaaaa agtgaccatt cactgcatca atcgccagcg gtttgccgaa
      601 gagatgcaga tcctgcgtga gattttcaat tctggctggc agcacaactg gggatttgtg
      661 ccgtttaccg aacatgaatt cgcgaccatg ggcgatcaac ttaaatatct ggtgccggac
      721 gatatgatct acatcgctga gattgattct gcaccctgcg cgtttattgt cggcttgccg
      781 aacatcaacg aggcgattgc cgatctgaac ggatcgctct ttcccttcgg ctgggcaaaa
      841 ttgctgtggc gtttgaaagt cagcggtgtg cgaactgcgc gagtgccgct gatgggcgtg
      901 cgggacgagt atcagttcag ccgcatcggc ccggtgattg cgctgttatt gattgaagcc
      961 ttacgcgatc cgtttgcccg ccggaagatc gatgcgctgg aaatgtcatg gatcctcgaa
     1021 accaatacag gcatgaataa catgctggaa cgcattgggg cggagccgta caagcgttac
     1081 cgattgtatg aaaagcagat ttgatgacag tcacccctgc ttcatctcca gcggcaccgc
     1141 cgagcgaatc aaatggtggc cttccagctc gatatcgtgg agattctttt tcacggtccg
     1201 cacatgatcg cgtgctgcgc gctgggcaac gtgcggcagc cgctgcaaca ccgcgttgta
     1261 gatccgcgca tgctggcgat cgatctgctt tttttgctgt ggtcgatggt agagattatt
     1321 tacgctggca aacaccgaat taaacatcag gtcggtcaat gattgcagcg taaacaccag
     1381 cacctgattg tgagaagcct gacaaatggc gagatggaaa gcgtgatcca actgcgcatg
     1441 ttcgatcagc gaaatctctt tgttgttctc actggcggcg agcatttttt cataacagcg
     1501 ggttatcaca acaaaatcag cctgggttcc cagtgttgcc gccagccttg ccgattcgcc
     1561 ctccagtaat gcgcgaacgt cgagcagatc gtacagcgtt cgcggctgcg tactgaacag
     1621 atggatcagc gggctggtgt cctgcacccg attaagccgt gcgacacgag aatcgcgacc
     1681 ctgtgccgtt tcaataatcc cgcgcccgcg cagcacggtc agcccttcac gcagtgcgga
     1741 gcgtgagaag ccgagctttt cacacagtcg acgttccgag ggaagcggct gaccgacctt
     1801 cagtacgccg tcgataatta accgttcgat actttctgca accacttcgc aaatagggcg
     1861 acgttcatct ttcattcccg gacctcgtgc acaggtagga ccaatttttc tgttcttaac
     1921 tcgcaaaaca cgcacatcac gtaagtgatt atgttacatc aatttaacat taagttaacc
     1981 aaagcaaggt cacagagctg gaaaaaaaat ggtctgaccg gtagctaaag agatagacga
     2041 aaacgaaaag cccgcttaat aactgttcac agaagcagcg cgaaaaaatc agctgccaca
     2101 caacacaaca aagcgaaccc tactcatgag catcttgtac gaagagcgtc ttgatggcgc
     2161 tttacccgaa gtcgaccgca catcggtact gatggcactg cgtgagcatg tccctagact
     2221 ggagatcctg cataccgatg aggagatcat tccttacgag tgtgacgggt tgagcgcgta
     2281 tcgcacgcgt ccattactgg ttgttctgcc taagcagatg gaacaggtga cggcgattct
     2341 ggctgtctgc caccgcctgc gtgtgccggt ggtgacccgt ggcgcaggca ccgggctttc
     2401 tggtggcgcg ctgccgctgg aaaaaggtgt gttgttggtg atggcgcgct ttaaagagat
     2461 cctcgacatt aaccccattg gtcgccgcgc gcgcgtgcag ccgggcgtgc gtaacctggc
     2521 gatctcccag gccgttgcgc cgcataatct ctattacgcg ccggatccgt cctcacaaat
     2581 cgcctgttcg attggcggca atgtggcgga aaatgccggt ggcgttcact gcctgaaata
     2641 tggtctaacc gtacataacc tgctgaaaat tgaagtgcaa acgctggatg gcgaggcgct
     2701 gacgctggga tcggacgcgc tggattcgcc tggttttgac ctgctggcgc tgtttaccgg
     2761 atcggaaggt atgctcggcg tgaccaccga agtaacggtg aagctgttgc cgaaaccgcc
     2821 cgtggcgcgg gttctgttag ccagctttga ctcggtagaa aaagccggac ttgcggttgg
     2881 tgacatcatc gccaatggca ttatccccgg cgggctggag atgatggata acctgtcgat
     2941 tcgcgcggcg gaagatttta ttcatgccgg ttatcccgtc gatgccgaag cgattttgtt
     3001 atgcgaactg gacggcgtgg agtctgacgt acaggaagac tgcgagcggg ttaacgacat
     3061 cttgttgaac gcgggtgcga ctgacgtccg tctggcacag gacgaagcag agcgcgtacg
     3121 tttctgggcc ggtcgcaaaa atgcgttccc ggcggtagga cgtatctccc cggattacta
     3181 ctgcatggat ggcaccatcc cgcgccgcgc cctgcctggc gtactggaag gcattgcccg
     3241 tttatcgcag caatatgatt tacgcgttgc caacgtcttt catgccggag acggcaacat
     3301 gcacccgtta atccttttcg atgccaacga acccggtgaa tttgcccgcg cggaagaact
     3361 gggcgggaag atcctcgaac tctgcgttga agttggcggc agcatcagtg gggaacatgg
     3421 catcgggcgc gaaaaaatca atcaaatgtg cgcccagttc aacagcgatg aaatcacgac
     3481 cttccatgcg gtcaaggcgg cgtttgaccc cgatggtttg ctgaacccag gaaaaaacat
     3541 tcccacgcta caccgctgtg ctgaatttgg tgccatgcat gtgcatcacg gtcatttacc
     3601 ttttcctgaa ctggagcgtt tctgatgcta cgcgagtgtg attacagcca ggcgctgctg
     3661 gagcaggtga atcaggcgat tagcgataaa acgccgctgg tgattcaggg cagcaatagc
     3721 aaagcctttt taggtcgccc tgtcaccggg caaacgctgg atgtgcgttg tcatcgcggc
     3781 attgttaatt acgacccgac cgagctggtg ataaccgcgc gtgccggaac gccgctggtg
     3841 gcgattgaag cagcgctgga gagcgccggg caaatgctcc cctgcgagcc gccgcattat
     3901 ggtgaagaag ccacctgggg cgggatggtc gcttgcgggc tggcggggcc gcgtcgcccg
     3961 tggagtggtt cggtccgcga ttttgtcctc ggcacgcgca tcattaccgg cactggaaaa
     4021 catctgcgtt ttggtggcga agtgatgaaa aacgttgccg gatacgatct ctcacggtta
     4081 atggccggaa gctacggttg ccttggcgtg ctcactgaaa tctcgatgaa agtgttaccg
     4141 cgaccgcgcg cctccctgag cctgcgtcgg gaaattagcc tgcaagaagc catgaatgaa
     4201 atcgcccagt ggcaactcca gccattaccc gttagtggct tatgttactt cgacaatgcg
     4261 ttgtggatcc gccttgaggg cggcgaagga tcggtaaaag cagcgcgtga actgctgggt
     4321 ggcgaagagg ttgcaggtca gttctggcag caattgcgtg aacaacaact gccgttcttc
     4381 tcgttaccag gtaccttatg gcgcatttca ttacccagtg atgcgccgat gatggattta
     4441 cccggcgagc aactgatcga ctggggcggg gcgttacgct ggctgaaatc gacagccgat
     4501 gataatcaaa tccatcgcat cgcccgcaac gcgggcggtc atgcgacccg ctttagtgcc
     4561 ggaaacggtg gctttgcccc gctaccggca cctttattcc gctatcacca gcagcttaaa
     4621 cagcagctcg atccttgcgg cgtgtttaac cccggtcgca tgtacgcgga actttgagga
     4681 gcaggctatg caaacccgat taacagaaga gatgcggcag aacgcgcgcg cgctggaagc
     4741 cgacagcatc ctgcgcgcct gtgttcactg cggattttgt accgcaacct gcccaaccta
     4801 tcagcttctg ggcgatgaac tggacgggcc gcgcgggcgc atctatctga ttaaacaggt
     4861 gctggaaggc aacgaagtca cgcttaaaac acaggagcat ctcgatcgct gtcttacttg
     4921 ccgcaattgt gaaactacct gtccttctgg tgtgcgttat cacaatttgc tggatatcgg
     4981 gcgtgatatt gtcgagcaga aagtgaaacg cccactgccg gagcgaatgc tgcgcgaagg
     5041 attgcgccag gtagtgccgc gtccggcggt cttccgtgcg ctgacgcagg tagggctggt
     5101 gctgcgaccg tttttaccgg aacaggtcag agcaaaactg cctgctgaaa ccgtgaaagc
     5161 taaaccgcgt ccaccactgc gccataagcg tcgggtttta atgctggaag gctgcgccca
     5221 gcctacgctt tcgcctaaca ccaacgcggc aactgcgcga gtgctggatc gtctggggat
     5281 cagcgtcatg ccagctaacg aagcaggctg ttgtggcgcg gtggactatc atcttaatgc
     5341 gcaagagaaa gggctggcgc gggcgcgcaa taatattgat gcctggtggc ccgcgattga
     5401 agcaggtgcc gaggcaattt tgcaaaccgc cagcggctgc ggcgcgtttg tcaaagagta
     5461 tgggcagatg ctgaaaaacg atgcgttgta tgccgataaa gcgcgtcagg tcagtgagct
     5521 ggcggtcgat ttagtcgaac ttctgcgcga ggaaccgctg gaaaaactgg caattcgcgg
     5581 cgataaaaaa ctggccttcc actgcccgtg taccctacaa catgcgcaaa agctgaacgg
     5641 cgaagtggaa aaagtgttgc ttcgtcttgg atttacctta atggacgttc ccgacagcca
     5701 tctgtgctgc gggtcggcgg gaacatatgc gttaacgcat cccgatctgg cgcgccagct
     5761 gcgggataac aaaatgaatg cgctggaaag cggcaaaccg gaaatgatcg tcaccgccaa
     5821 cattggttgc cagacgcatc tggcgagcgc cggtcgtacc tctgtgcgtc actggattga
     5881 aattgtagag caagcccttg aaaaggaata acaaaatgaa aactaaagtc attcttagcc
     5941 agcaaatggc gagtgcaatt attgccgcag gtcaggaaga ggcgcagaaa aataactggt
     6001 ctgtttccat tgctgttgcc gatgacggcg gtcatctgct ggcgttaagt cgcatggacg
     6061 attgcgcgcc gattgcggct tacatctccc aggagaaagc gcgtaccgcc gcgctggggc
     6121 gtcgtgaaac taagggctat gaagagatgg tgaacaacgg acgtaccgcg ttcgtgactg
     6181 cgccgttact gacgtcgctg gaaggtggcg taccggttgt tgtggatggg caaattattg
     6241 gtgccgtggg cgtttctggt ttaaccggag cacaggatgc gcaggtcgcg aaagcggcag
     6301 cagcggtgtt ggcgaaataa gcgaaaacga ggagataaac aatgagtcaa accataaccc
     6361 agggccgttt acgcattgac gccaatttta aacgttttgt ggatgaagaa gttttgcctg
     6421 gcgtggaact ggatgctgcc gcgttctggc acaatgttga tgagatcgtt cacgatctgg
     6481 cgccagagaa tcgtcagttg ctggcagagc gcgatcgcat tcaggcggcg cttgatgagt
     6541 ggcatcgcag caatccgggg ccggtaaaag ataaagcggc ctataaatct ttcctgcgtg
     6601 aactgggcta cctggtgcca cagcctgacc acgttacggt ggaaaccacg ggcatcgaca
     6661 gcgaaatcac cagtcaggcg gggccacagc tggtggttcc ggcgatgaac gcccgctacg
     6721 cgctgaacgc ggcgaacgct cgctggggct cactgtacga tgcgttatac ggcagcgaca
     6781 tcatcccgca ggaaggggcg atggtcagcg gctacgatcc gcagcgtggt gagcaggtta
     6841 tcgcctgggt tcggcgtttc ctcgatgaat cactaccgct ggaaaacggc agctatcagg
     6901 atgtggtggc gtttaaggtg gtcgataaac aattacgcat ccagttgaaa aatggtaaag
     6961 aaaccacgtt acgtactcca gcacagtttg tcggttaccg tggcgatact actgcgctga
     7021 cctgcatttt gctgaaaaat aacggcctgc atattgagct acaaatcgat gccaacgggc
     7081 ggattggcaa agacgatccg gcgcacatca acgatgttat cgtcgaagcg gccatcagta
     7141 ccattctcga ctgcgaagat tcggtcgcgg cggttgatgc ggaagataaa atcctgctgt
     7201 atcgcaacct gctgggcctg atgcagggga ctctgcaaga gaaaatggag aagaacggtc
     7261 ggcaaatcgt gcgtaaactg aatgacgatc gtcagtacac tgccgccgat ggatctgaaa
     7321 tttctctgca cggacgctcg ttgctgttta tccgcaacgt gggtcatttg atgaccattc
     7381 ctgtgatttg ggacagcgaa ggcaatgaaa tcccggaagg cattcttgat ggcgtcatga
     7441 ctggcgcgat tgccctctat gatttaaaag tgcagaaaaa ctcgcgcact ggcagcgtct
     7501 atattgtgaa accgaaaatg cacggtccgc aggaagtggc gttcgccaac aaactgtttt
     7561 cccgcgttga gacaatgctc ggtatggcac cgaataccct gaaaatgggc attatggatg
     7621 aagaacgccg gacctcgctg aacttgcgta gctgtatcgc tcaggcgcgc aaccgcgtgg
     7681 cgttcatcaa taccggtttc ctcgaccgta ccggcgatga aatgcattcg gtgatggaag
     7741 cgggcccgat gctgcgtaaa aaccagatga aatcgacgcc gtggatcaaa gcctacgaac
     7801 gtaataacgt gctttccggt ctgttctgtg ggctacgtgg aaaagcgcaa atcggtaaag
     7861 gcatgtgggc aatgccggac ctgatggcag acatgtacag ccagaagggc gaccaattgc
     7921 gtgccggggc aaacacggcc tgggttccgt cgccaactgc cgctacgctc catgcgctgc
     7981 actaccacca aaccaacgta cagagcgtgc aagccaacat tgcccagacc gagttcaatg
     8041 ctgaatttga accgctgctg gacgatctgc tgacgattcc ggttgctgaa aacgctaact
     8101 ggtcggcgca agagatccaa caagagctgg ataacaacgt gcaggggatt ctggggtacg
     8161 tggtgcgttg ggtggagcag gggattggtt gttcaaaagt gccggatatt cacaacgtgg
     8221 cgttgatgga agaccgcgca acgctgcgta tttccagcca gcatatcgcc aactggttac
     8281 gtcacggtat tctgaccaaa gatcaggtgc aggcgtcgct ggagaatatg gcgaaagtgg
     8341 ttgatcagca aaacgctggc gatccggctt atcgtccgat ggcagggaat ttcgctaact
     8401 cgtgtgcttt taaagctgcc tgcgatttga tcttcctcgg cgtgaaacag ccaaacggct
     8461 ataccgaacc gttattacac gcatggcgtt tacgcgaaaa agaaaatcat taatagcgtc
     8521 aaaaaaagcc ccggcaagat ttattgtcgg ggctatgata gtaactgcgg tagtacattt
     8581 atcccggaaa taaccggagc gattatcagg ggttaacaat attaaatacc cgtagcgcat
     8641 cactagcggg attaaaacaa caatatggac atcttaacga tactctgcgc ctgcgtgtgc
     8701 ggaacagaaa cccttacccg atatacttgt tatgccagag gtagcaattg cgttagcggc
     8761 gtactgttgc tgtaaagtat aacgcctata ttttacagtt cagtcctctt ttgcttatta
     8821 caaccttaag tatatcaagc atataaagat aataagagac tgaacaatat ggttacctgg
     8881 acccaaatgt atatgccgat gggaggactg gggctatccg ctctggtcgc cctgatccca
     8941 ataatattct tcttcgttgc actcgcggta ttacgtctga aaggacatgt cgccggagca
     9001 ataaccctta tattatctat tctgattgcg atattcgcct ttaaaatgcc gattgatatg
     9061 gcatttgctg ctgcgggcta tggctttatt tatggtttat ggcctattgc gtggattatt
     9121 gtcgcggcgg tattcctgta taaattaacc gttgccagcg gccagttcga tattatccgc
     9181 agctcggtta tctccatcac cgacgatcag cgtttgcagg tgttactgat tggtttctcc
     9241 tttggtgcgc tgctggaagg ggcggctggc tttggcgcac cggtggcgat taccggtgct
     9301 ctactggtgg gcctgggctt caaaccgcta tacgcggcgg ggctgtgtct gattgccaac
     9361 actgcgccgg tggcgtttgg tgcgttgggt gtgccaattc tggtcgccgg tcaggtaacg
     9421 ggaatcgatc cgttcgacat tggcgcaatg gcgggacgtc agttaccgtt cctgtcggtt
     9481 cttgtgccgt tctggctggt agcaatgatg gacggctgga aaggggtgaa agagacgtgg
     9541 ccagcggcgc tggttgcagg gggaagcttc gctgtcactc agttctttac ctctaactat
     9601 attggtccgg aactgccgga tattacttcg gcgctggtga gtatcgtctc actttcttta
     9661 tttcttaaag tgtggcggcc aaaaaatacc gaaacggcaa tcagtatggg acaatccgca
     9721 ggtgcgatgg tggtgaataa accgtcttct ggcggtcccg tgccttcaga atatcgtctg
     9781 gggcaaatca ttcgtgcgtg gtcaccgttt ttaatcttaa cggtgttggt caccatctgg
     9841 accatgaagc cgtttaaagc gttatttgct ccgggcggcg cgttttattc actggtgatt
     9901 aatttccaga tccctcattt gcatcaacaa gtcttgaaag cggcccccat tgtcgcccag
     9961 ccgacgccaa tggacgcggt gtttaaattc gaccccctct ctgctggtgg caccgctatt
    10021 tttattgcgg cgattatctc gatcttcatc ctcggcgtgg ggatcaagaa aggtattggc
    10081 gtctttgccg aaacgctaat tagtctgaag tggccgatat tgtcgattgg catggtgctg
    10141 gcatttgcct tcgtgaccaa ctattccggc atgtccacca cgttggcgct ggtactggct
    10201 ggtacaggcg tgatgttccc gttcttctca ccgttcctcg gctggctggg cgtattcctt
    10261 accggctctg acacttcttc aaacgccctg tttggttcac tgcaatcgac cacggcgcag
    10321 caaatcaacg tctctgacac cctgctggtg gcagcaaaca ccagcggcgg cgtaactggc
    10381 aagatgatct ccccgcaatc tatcgccgtg gcctgcgccg cgacgggcat ggtgggtcga
    10441 gaatctgaac tgttccgcta caccgtgaag cacagtctga tttttgccag cgttatcggc
    10501 attatcacgc tgttgcaggc gtatgtgttt accgggatgt tagtttcgta agtcaataaa
    10561 gccggatgat cgttaatatc atccggcttc gtttacatta ttctgtctta ttaatggaaa
    10621 attatttcct gctttattat ccatgcggca ataatgtttt gcgttcagat aacatgctaa
    10681 ataattaaga gcgtgagaac atctattttt tatcacgcaa aacaagtctg tattaattat
//
LOCUS       DADSYM010000152        10626 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2415
            SAMN08039579-rid11723173.denovo.153, whole genome shotgun sequence.
ACCESSION   DADSYM010000152 DADSYM010000000
VERSION     DADSYM010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039579
            Sequence Read Archive: SRR6362533
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10626)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10626)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980162.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 03:39:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,484
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 4,347
            CDSs (with protein)               :: 4,347
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 43 of 85
            Pseudo Genes (incomplete)         :: 37 of 85
            Pseudo Genes (internal stop)      :: 19 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10626
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039579-rid11723173.denovo.153"
                     /strain="LGE2415"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8302 N 95.9628 W"
                     /collection_date="2015-08-24"
     gene            95..1204
                     /gene="anmK"
                     /locus_tag="J1Y83_003659"
     CDS             95..1204
                     /gene="anmK"
                     /locus_tag="J1Y83_003659"
                     /EC_number="2.7.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HBA7631771.1"
                     /translation="MKSGRFIGVMSGTSLDGVDVVLATIDEHRVAQLASLSWPIPVSL
                     KQAVLDICQGQQLTLSQFGQLDTQLGRLFADAVNALLKEQNLQARDIVAIGCHGQTVW
                     HEPTGVAPHTLQIGDNNQIVARTGITVVGDFRRRDIALGGQGAPLVPAFHHALLAHPT
                     ERRMVLNIGGIANLSLLIPGQPVGGYDTGPGNMLMDAWIWRQAGKPYDKDAEWARAGK
                     VILPLLQNMLCDPYFSQPAPKSTGREYFNYGWLERHLRHFPGVDPRDVQATLAELTAV
                     TISEQVLLSGGCERLMVCGGGSRNPLLMARLAALLPGTEVTTTDAVGISGDDMEALAF
                     AWLAWRTLAGLPGNLPSVTGASQETVLGAIFPANP"
     gene            1302..1631
                     /gene="mliC"
                     /locus_tag="J1Y83_003660"
     CDS             1302..1631
                     /gene="mliC"
                     /locus_tag="J1Y83_003660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416156.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C-type lysozyme inhibitor"
                     /protein_id="HBA7631772.1"
                     /translation="MSMKKLLLICLPVLLTGCSAFNQLVERMQTDTLEYQCDEKPLTV
                     KLNNPRQEVSFVYDNQLLHLKQGISASGARYTDGIYVFWSKGEEATVYKRDRIVLNNC
                     QLQNPQR"
     gene            1690..2346
                     /gene="pdxH"
                     /locus_tag="J1Y83_003661"
     CDS             1690..2346
                     /gene="pdxH"
                     /locus_tag="J1Y83_003661"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase"
                     /protein_id="HBA7631773.1"
                     /translation="MSDNDELQQIAHLRREYTKGGLRRRDLPADPLTLFERWLSQACE
                     AKLADPTAMVVATVDEHGQPYQRIVLLKHYDEKGMVFYTNLGSRKAHQIENNPRVSLL
                     FPWHTLERQVMVIGKAERLSTLEVMKYFHSRPRDSQIGAWVSKQSSRISARGILESKF
                     LELKQKFQQGEVPLPSFWGGFRVSLEQIEFWQGGEHRLHDRFLYQRENDAWKIDRLAP
                     "
     gene            2475..3749
                     /gene="tyrS"
                     /locus_tag="J1Y83_003662"
     CDS             2475..3749
                     /gene="tyrS"
                     /locus_tag="J1Y83_003662"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HBA7631774.1"
                     /translation="MASSNLIKQLQERGLVAQVTDEEALAERLAQGPIALYCGFDPTA
                     DSLHLGHLVPLLCLKRFQQAGHKPVALVGGATGLIGDPSFKAAERKLNTEETVQEWVD
                     KIRKQVAPFLDFDCGENSAIAANNYDWFGNMNVLTFLRDIGKHFSVNQMINKEAVKQR
                     LNREDQGISFTEFSYNLLQGYDFACLNKQYGVVLQIGGSDQWGNITSGIDLTRRLHQN
                     QVFGLTVPLITKADGTKFGKTEGGAVWLDPKKTSPYKFYQFWINTADADVYRFLKFFT
                     FMSIEEINALEEEDKNSGKAPRAQYVLAEQVTRLVHGEEGLQAAKRITECLFSGSLSA
                     LSEADFEQLAQDGVPMVEMEKGADLMQALVDSELQPSRGQARKTIASNAITINGEKQS
                     DPEYFFKEEDRLFGRFTLLRRGKKNYCLICWK"
     gene            3811..4671
                     /gene="pdxY"
                     /locus_tag="J1Y83_003663"
     CDS             3811..4671
                     /gene="pdxY"
                     /locus_tag="J1Y83_003663"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310372.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal kinase PdxY"
                     /protein_id="HBA7631775.1"
                     /translation="MKNILAIQSHVVYGHAGNSAAEFPMRRLGANVWPLNTVQFSNHT
                     QYGKWTGCVMPPSHLTEIVQGIAAIDKLHTCDAVLSGYLGSAEQGEHILGIVRQVKAA
                     NPQAKYFCDPVMGHPEKGCIVAPGVAEFHVRHGLPASDIIAPNLVELEILCEHPVNNV
                     EEAVLAARELIAQGPQIVLVKHLARAGYSRDRFEMLLVTADEAWHISRPLVDFGMRQP
                     VGVGDVTSGLLLVKLLQGATLQEALEHVTAAVYEIMVTTKAMQEYELQVVAAQDRIAN
                     PEHYFSATKL"
     gene            complement(4715..5320)
                     /gene="gstA"
                     /locus_tag="J1Y83_003664"
     CDS             complement(4715..5320)
                     /gene="gstA"
                     /locus_tag="J1Y83_003664"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione transferase GstA"
                     /protein_id="HBA7631776.1"
                     /translation="MKLFYKPGACSLASHITLRESGKDFTLVSVDLMKKRLENGDNYF
                     AVNPKGQVPALLLDDGTLLTEGVAIMQYLADSVPDRQLLAPVNSISRYKTIEWLNYIA
                     TELHKGFTPLFRPDTPEEYKSTVRAQLEKKLQYVNEALKDEHWICGQRFTIADAYLFT
                     VLRWAYAVKLNLEGLEHIAAFMQRMAERPEVQDALSAEGLK"
     gene            complement(5425..6927)
                     /gene="dtpA"
                     /locus_tag="J1Y83_003665"
     CDS             complement(5425..6927)
                     /gene="dtpA"
                     /locus_tag="J1Y83_003665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpA"
                     /protein_id="HBA7631777.1"
                     /translation="MSTANQKPTESVSLNAFKQPKAFYLIFSIELWERFGYYGLQGIM
                     AVYLVKQLGMSEADSITLFSSFSALVYGLVAIGGWLGDKVLGTKRVIMLGAIVLAIGY
                     ALVAWSGHDAGIVYMGMAAIAVGNGLFKANPSSLLSTCYEKNDPRLDGAFTMYYMSVN
                     IGSFFSMIATPWLAAKYGWSVAFALSVVGLLITIVNFAFCQRWVKQYGSKPDFEPINY
                     RNLLLTIIGVVALIAIATWLLHNQEVARMALGVVAFGIVVIFGKEAFAMKGAARRKMI
                     VAFILMLEAIIFFVLYSQMPTSLNFFAIRNVEHTILGLAVEPEQYQALNPFWIIIGSP
                     ILAAIYNKMGDTLPMPTKFAIGMVMCSGAFLILPLGAKFASDAGIVSVSWLVASYGLQ
                     SIGELMISGLGLAMVAQLVPQRLMGFIMGSWFLTTAGANLIGGYVAGMMAVPDNVTDP
                     LMSLEVYGRVFLQIGVATAVIAVLMLFTAPKLHRMTQDDAADKAAKAAVA"
     gene            complement(7538..8173)
                     /gene="nth"
                     /locus_tag="J1Y83_003666"
     CDS             complement(7538..8173)
                     /gene="nth"
                     /locus_tag="J1Y83_003666"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HBA7631778.1"
                     /translation="MNKAKRLEILTRLRENNPHPTTELNFSSPFELLIAVLLSAQATD
                     VSVNKATAKLYPVANTPAAMLELGVEGVKTYIKTIGLYNSKAENIIKTCRILLEQHNG
                     EVPEDRAALEALPGVGRKTANVVLNTAFGWPTIAVDTHIFRVCNRTQFAPGKNVEQVE
                     EKLLKVVPAEFKVDCHHWLILHGRYTCIARKPRCGSCIIEDLCEYKEKVDI"
     gene            complement(8173..8868)
                     /locus_tag="J1Y83_003667"
     CDS             complement(8173..8868)
                     /locus_tag="J1Y83_003667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit E"
                     /protein_id="HBA7631779.1"
                     /translation="MSEIKDVIVQGLWKNNSALVQLLGLCPLLAVTSTATNALGLGLA
                     TTLVLTLTNLTISTLRHWTPAEIRIPIYVMIIASVVSAVQMLINAYAFGLYQSLGIFI
                     PLIVTNCIVVGRAEAFAAKKGPALSALDGFSIGMGATCAMFVLGSLREIIGNGTLFDG
                     ADALLGSWAKVLRVEIFHTDSPFLLAMLPPGAFIGLGLMLAGKYLIDEKMKKRRTEAA
                     AERALPNGETGNV"
     gene            complement(8872..9492)
                     /gene="rsxG"
                     /locus_tag="J1Y83_003668"
     CDS             complement(8872..9492)
                     /gene="rsxG"
                     /locus_tag="J1Y83_003668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxG"
                     /protein_id="HBA7631780.1"
                     /translation="MLKTIRKHGITLALFAAGSTGLTAAINQMTKTTIAEQASLQQKA
                     LFDQVLPAERYNNALAQSCYLVTAPELGKGEHRVYIAKQDDKPVAAVLEATAPDGYSG
                     AIQLLVGADFNGTVLGTRVTEHHETPGLGDKIELRLSDWITHFAGKKISGADDANWAV
                     KKDGGDFDQFTGATITPRAVVNAVKRAGLYAQTLPAQLSQLPACGE"
     gene            complement(9496..10554)
                     /gene="rsxD"
                     /locus_tag="J1Y83_003669"
     CDS             complement(9496..10554)
                     /gene="rsxD"
                     /locus_tag="J1Y83_003669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxD"
                     /protein_id="HBA7631781.1"
                     /translation="MVFRIASSPYTHNQRQTSRIMLLVLLAAVPGIAAQLWFFGWGTL
                     VQILLASVSALLAEALVLKLRKQSVAATLKDNSALLTGLLLAVSIPPLAPWWMVVLGT
                     VFAVIIAKQLYGGLGQNPFNPAMIGYVVLLISFPVQMTSWLPPHEIAVNIPGFIDAIQ
                     VIFSGHTTSGGDMNTLRLGIDGISQATPLDTFKTSVRAGHSVEEIMQYPIYSGILAGA
                     GWQWVNLAWLAGGVWLLWQKAIRWHIPLSFLVTLALCATLGWLFSPDTLAAPQIHLLS
                     GATMLGAFFILTDPVTASTTNRGRLIFGALAGLLVWMIRSFGGYPDGVAFAVLLANIT
                     VPLIDYYTRPRVYGHRKG"
BASE COUNT         2707 a         2652 c         2722 g         2545 t
ORIGIN      
        1 actaccaacg gtaaaaattg gtcattccgc atggtattca cgaaaagttt atgtttgagt
       61 ggtgcgcaaa cgtcagattg aaaaaggatg aactatgaaa tcgggccgct ttattggcgt
      121 tatgtcaggc accagccttg atggtgttga tgttgtgttg gcgacaattg atgaacaccg
      181 ggtcgcacag ctggcaagtt tgagctggcc gatcccggtg tctctgaaac aggctgtact
      241 ggatatttgc cagggccagc agcttacact ttcgcagttt ggacagcttg atactcaact
      301 tgggcgactt tttgctgatg cggtcaatgc cttgcttaag gaacaaaacc tgcaggcgag
      361 agatatagtt gcgatcggct gccacggtca aaccgtctgg cacgaaccga cgggcgtggc
      421 accacacact ttacagattg gcgataacaa tcaaattgtg gcgcgcaccg gaattacggt
      481 tgtcggtgat tttcgccgtc gcgatattgc cttgggagga caaggtgcac cgctggtacc
      541 tgcgttccat catgcgctgc tggctcaccc aaccgagcga cgaatggtgc tcaatattgg
      601 cggcatcgcc aatctgtcac tgctcattcc tggccagccg gttgggggct acgataccgg
      661 tcctggtaac atgctgatgg atgcctggat ctggcgtcag gccggtaaac cttacgataa
      721 agatgccgag tgggcacggg cgggtaaagt tattctccca ctgctgcaaa atatgctctg
      781 cgacccatat ttctcgcaac ccgcaccgaa aagcaccgga cgcgaatact ttaactacgg
      841 ctggctggag cgccatttgc gccattttcc gggggttgat ccccgtgatg tgcaggccac
      901 actggcagaa ctcaccgccg tgaccatttc tgaacaagtt ttgttgagcg gtggctgcga
      961 acgattgatg gtatgtggtg gaggtagtcg taatccgcta ctcatggcgc gtctggcggc
     1021 attactgcca ggcacagaag tcaccaccac cgatgccgtt ggcattagtg gcgatgacat
     1081 ggaagcattg gcgttcgcct ggcttgcttg gcgaacgctg gcgggattac caggaaatct
     1141 gccttccgtc actggcgcaa gccaggagac ggtactgggg gctattttcc ccgctaaccc
     1201 gtgatatcag agttaactga atttttctta ccgcgtctgc cgttacactt ggaacgttag
     1261 ggagggcgta ttgccctcca gaccaggaaa gtcttcggga tatgtctatg aaaaaactgt
     1321 tactgatctg tttgccggtg cttctcaccg gctgtagcgc gttcaatcaa ctggttgagc
     1381 gcatgcaaac cgatacgctg gaataccagt gtgatgaaaa accgttgacg gtcaaactga
     1441 ataatccgcg ccaggaagtc agttttgttt acgataatca actactgcat ctcaaacagg
     1501 gcatttcagc gtctggtgca cgttacaccg acggaatcta tgttttctgg tcgaaaggcg
     1561 aggaagcgac tgtctataaa cgcgaccgca tcgtcttgaa taactgtcag ttacaaaatc
     1621 cgcagcgttg agatttttcc aggggcggcg cacaatagcg tcacccactg acaatccgta
     1681 aagaaaacca tgtctgataa cgacgaattg cagcaaatcg cgcatctgcg ccgtgaatac
     1741 accaaaggcg ggttacgccg ccgcgatcta cccgccgatc cattaaccct ttttgaacgt
     1801 tggctctctc aggcttgtga agccaaactg gctgacccta ccgcgatggt ggtcgctacc
     1861 gtggatgaac atggtcagcc ttatcagcgc atcgttttac tcaaacatta cgacgaaaaa
     1921 ggcatggtgt tttacaccaa cctcggcagc cgtaaagcac atcaaatcga aaataatccg
     1981 cgcgttagcc tgctgttccc gtggcatacc cttgaacgcc aggtgatggt gatcggtaaa
     2041 gcggaacgac tttcgactct cgaagtgatg aaatattttc atagccgccc gcgtgatagc
     2101 cagattggtg catgggtttc gaagcagtcc agtcgcattt ctgcccgcgg tatccttgaa
     2161 agtaaattcc tggagctgaa gcagaagttt caacagggcg aagtgccatt gccaagtttt
     2221 tggggtggtt ttcgcgtcag ccttgaacag attgagttct ggcagggcgg tgagcatcgc
     2281 ctgcatgacc gctttttgta ccagcgtgaa aatgatgcgt ggaagattga tcgtcttgca
     2341 ccctgaaaag atgcaaaaat cttgctttaa tcgctggtac tcctgattct ggcactttat
     2401 tctatgtctc tttcgcatct ggcgaaaagt cgtgtaccgg caaaggtgca gtcgttatat
     2461 acatggagat tttgatggca agcagtaact tgattaaaca attgcaagag cgggggctgg
     2521 tagcccaggt gacggacgag gaagcgttag cagagcgact ggcgcaaggc ccgatcgcgc
     2581 tctattgcgg cttcgatcct accgctgaca gcttgcattt ggggcatctt gttccattgt
     2641 tatgcctgaa acgcttccag caggcgggcc acaagccggt tgcgctggta ggcggcgcga
     2701 cgggtctgat tggcgacccg agcttcaaag ctgccgagcg taagctgaac actgaagaaa
     2761 ctgttcagga gtgggtggac aaaatccgca agcaggttgc cccgttcctc gatttcgact
     2821 gtggagaaaa ctctgctatc gcggcgaaca actatgactg gttcggcaat atgaatgtgc
     2881 tgaccttcct gcgcgatatt ggcaaacact tctccgttaa ccagatgatc aacaaagaag
     2941 cggttaagca gcgtctcaac cgtgaagatc aggggatttc gttcactgag ttttcctaca
     3001 acctgttgca gggttatgac ttcgcctgtc tgaacaaaca gtacggtgtg gtgctgcaaa
     3061 tcggtggttc tgaccagtgg ggtaacatca cttctggtat cgacctgacc cgtcgtctgc
     3121 atcagaatca ggtgtttggc ttgaccgttc cgttgatcac caaagcagac ggcaccaaat
     3181 ttggtaaaac tgaaggcggc gcagtctggt tggacccgaa gaaaaccagc ccgtacaaat
     3241 tctaccagtt ctggatcaac actgcggatg ccgacgttta ccgcttcctg aaattcttca
     3301 ccttcatgag cattgaagag atcaacgccc tggaagaaga agataaaaac agcggtaaag
     3361 caccgcgcgc ccagtatgtg cttgcagaac aggtgacacg tctggtgcat ggtgaagaag
     3421 gtttgcaggc ggcaaaacgt attaccgaat gcctgttcag cggttccttg agtgcgctga
     3481 gtgaagcgga cttcgaacag ctggcgcagg acggcgtacc gatggttgag atggaaaagg
     3541 gcgcagacct gatgcaggcg ctggtcgatt ctgaactgca accttcccgt ggtcaggcgc
     3601 gtaaaactat cgcctccaat gccatcacca ttaacggtga aaaacagtcc gatcctgaat
     3661 atttctttaa agaagaagat cgtctgtttg gtcgttttac cttgctgcgt cgcggcaaaa
     3721 agaattactg tctgatttgc tggaaataat gcattaagtg gaaaggggga gtgagaaatc
     3781 actccccctg gtttttatac agggaacatg atgaaaaata ttctcgctat ccagtctcac
     3841 gttgtttatg gtcatgcggg taacagtgcg gcagagtttc cgatgcgccg cctgggcgcg
     3901 aacgtctggc cgctgaacac cgttcaattt tctaatcaca cccaatacgg caaatggact
     3961 ggctgtgtga tgccacccag ccatttaacc gaaattgtgc aaggcattgc cgccattgat
     4021 aaattacaca cctgtgatgc cgtattaagt ggctatctgg gatcggcgga gcagggtgaa
     4081 catatcctcg gtatcgttcg tcaggtgaaa gcagcgaatc cacaggcgaa atatttttgc
     4141 gatccggtaa tgggtcatcc ggaaaaaggc tgtatcgttg caccgggtgt cgcagagttt
     4201 catgtgcggc acggtttgcc tgccagcgat atcattgcgc cgaatctggt tgagctggaa
     4261 atactctgtg agcatccggt aaataacgtc gaagaagcgg ttctggcagc gcgcgaactc
     4321 attgcgcaag gaccacaaat tgtgttggtt aaacacctgg cgcgagctgg ctacagccgt
     4381 gaccgttttg aaatgctgct agtcaccgcc gatgaagcct ggcatatcag ccgtccgctg
     4441 gtggattttg gtatgcgcca gccggtaggt gttggtgatg tgacgagcgg cttactgctg
     4501 gtgaaactgc ttcagggggc aacgctgcag gaggccctgg aacatgtgac cgctgcagta
     4561 tacgaaatca tggtgaccac aaaagcaatg caggaatatg agctgcaagt ggttgctgct
     4621 caggatcgta ttgccaatcc agaacattat ttcagcgcaa caaagctctg aaatatctca
     4681 tttcggccca caacgacctg tgggccgatg gctattactt taagccttcc gctgacagcg
     4741 cgtcttgtac ttccggacgt tcagccatac gttgcataaa tgccgcaatg tgctctaacc
     4801 cttccagatt cagtttcacc gcgtatgccc agcgcagaac cgtaaacaga taggcatcag
     4861 caattgtaaa tcgttgcccg cagatccaat gctcatcctt cagtgcctcg ttcacatatt
     4921 gcagcttctt ctccagctgc gcgcgaactg tcgatttgta ctcttccggt gtatcagggc
     4981 gaaacagagg tgtgaaacct ttatgcagct cggtggcgat gtaattcagc cattcgatgg
     5041 ttttatagcg ggaaatactg tttaccggtg ccagcaactg gcggtcgggg acgctgtcgg
     5101 caagatactg cataatcgct acgccttccg tcagcaaagt accatcgtcc agcagcaatg
     5161 caggtacttg tcccttaggg ttaacggcaa agtaattgtc accgttttcg agacgttttt
     5221 tcattaaatc cacactgacg agcgtaaaat cctttccgct ctcacgcagg gtgatatggg
     5281 aagcgagaga gcaggcgccc ggtttgtaga acaatttcat cggtaactcc ttttgtctgt
     5341 ggtttaaagt atggtagtgc gttgctgaac aaaaaaaagc cgctaacgaa aagttagcgg
     5401 cttgtaaaag agtttccctg aattttacgc tacggctgct ttcgccgctt tgtctgcagc
     5461 gtcatcctgc gtcatgcggt gcagtttcgg cgcggtaaac agcatcagta ctgcaataac
     5521 ggcagtagcg acaccaatct gcaagaatac gcgaccatag acttccagtg acatcagcgg
     5581 atcggtaacg ttatccggca cagccatcat acctgcaaca taaccaccaa tcaggtttgc
     5641 accggcagtg gtcaggaacc agctacccat aatgaagccc atcagacgct gcggaacgag
     5701 ttgtgcaacc attgccagac ccaggccaga gatcattagt tccccgatac tctgcaggcc
     5761 atagctcgcg accagccagc ttacagacac gataccagca tcagacgcga atttcgcacc
     5821 cagcggcaga atcaggaacg caccagaaca catcaccatg ccgatagcaa acttggttgg
     5881 cattggcagg gtatcgccca tcttgttata gatagcggcc agaatcgggc taccaatgat
     5941 gatccagaac gggttcagtg cctgatactg ctcaggttct acggccaggc ccagaatggt
     6001 gtgttcaacg ttacgaatcg caaagaagtt cagtgacgtt ggcatctggc tataaagcac
     6061 gaagaagata atggcttcga gcatcaggat gaaggcaacg atcattttac gacgcgcagc
     6121 acctttcatc gcgaaggctt ctttaccgaa gataaccacg ataccgaagg caacaacgcc
     6181 cagcgccata cgcgcaactt cctgattgtg cagcagccag gtggcgatag cgatcagtgc
     6241 cacaacacca ataatggtca gcagcaggtt acggtagttg ataggctcga agtctggttt
     6301 tgaaccgtac tgtttaaccc aacgttggca gaaggcgaag ttaacgatag tgatcagcag
     6361 gcctacaacg ctcaacgcaa acgcaacact ccagccgtat ttcgcggcca gccacggcgt
     6421 agcaatcata gagaagaaag agccgatgtt gacggacatg tagtacatgg tgaatgcacc
     6481 gtccagacgc gggtcgtttt tctcatagca tgtagaaagc agagaagacg ggttagcttt
     6541 aaacaggccg ttaccgaccg caatagccgc catacccata taaacgatac cggcgtcgtg
     6601 accagaccag gcaaccagag cataaccaat cgccagcaca atagcgccga gcataattac
     6661 gcgtttagta cccagtacct tgtcacctaa ccagccgccg atagcgacca gaccataaac
     6721 cagggcacta aaggaagaga aaagggtgat tgaatccgct tcagacatac ccagttgttt
     6781 aaccaggtaa acagccataa ttccttgtag gccgtaataa ccaaaacgtt cccataactc
     6841 aatcgagaag atgagataga acgccttcgg ttgtttaaaa gcgttcaaac tgacgctttc
     6901 agttggtttt tggtttgcag tggacacttt tacctctttt tttacatccc atattaacgg
     6961 gggtgctttc tcgcgttatg tctaaaaaac atacgcgcaa ttgttatagt gggaggggaa
     7021 acggcaggta atgttcacta tcttgcactt tcagacaagt cctttgtaat aatctgttac
     7081 atataactga ggcggggaat tcaaccattc cgggaaatgt tatccagcgt taaatttttc
     7141 aaaaaacgta acggcagatt ttttcactac cctgagaggg tgatgatggc ttaatggcga
     7201 tatgttctac tatttggcgt ttttcctagt gagatagtcc agtttctgaa aaatagccag
     7261 tgtaatgttt tgtaggtcaa tatttgttgc gtgtgtaaat ttaaatgatt ttaacctgat
     7321 tcttgcaatg tttttacatt gccacagcat cgtgatcttg atcacgaatg aatagaaaat
     7381 ttcgctatta aaaaaacaat taacctgcat ttttggttat ctgcaaacgg attacctgat
     7441 gataacgcta agcaatggca tcattgacgt taaaaagatg ttttaaaaga ggataaagaa
     7501 aggttatgaa tggggtaatc ggtgttaccc ctgatcttta gatgtcaact ttctctttgt
     7561 attcacaaag atcttcaata atacaagagc cacagcgagg cttgcgggca atgcaggtat
     7621 aacgcccgtg caggatcaac caatggtggc agtcgacttt aaactctgcc ggaaccactt
     7681 tcagtagctt ttcttctacc tgttcgacgt ttttccccgg cgcaaattgt gtacgattac
     7741 aaacgcggaa aatgtgcgtg tcgacagcaa tagtcggcca gccgaatgca gtgtttaata
     7801 cgacgttggc tgttttacga cctacaccag gcagggcttc aagcgcagca cgatcttccg
     7861 gaacctcgcc attatgttgt tccagtaata tacggcaggt tttgatgata ttttctgctt
     7921 tgctgttata aaggccaatc gttttgatat aggttttcac cccttcaacg cccagttcaa
     7981 gcatcgctgc aggcgtatta gccaccgggt agagtttcgc cgtcgcctta ttaacactga
     8041 catcggtcgc ctgagcggaa agcagtacgg caatcagcaa ttcaaaaggc gaactgaaat
     8101 taagctcggt ggtgggatga ggattgttct cacgcaggcg agtgaggatc tccaggcgtt
     8161 ttgctttgtt catcagacat tccctgtttc accgtttggc aatgcacgtt cggcagctgc
     8221 ttcagtacgg cgctttttca ttttttcatc aatcaggtat tttcctgcca gcatcagtcc
     8281 caggccaata aatgcacctg gtggcagcat cgccagcagg aaaggggagt cggtgtggaa
     8341 aatctccacg cgtaatactt tcgcccagct acctaacagc gcatctgcac cgtcaaacaa
     8401 cgtgccattg ccgataattt cgcgtagtga acccagcacg aacatggcgc aggttgcgcc
     8461 cataccaatt gaaaagccgt ccagtgccga aagcgccgga ccttttttgg cggcgaaggc
     8521 ttcagcgcgg cccacaacga tacagttagt gacaatcagc ggaataaaaa tccctaatga
     8581 ttgatacagg ccaaaggcgt aggcgttgat cagcatctgt acagcgctga ccaccgaggc
     8641 gatgatcatc acgtaaatgg gaatgcggat ctcggcgggc gtccagtgac gcagcgtcga
     8701 aatggtcagg ttggttagcg tcagtaccag cgtagtcgca agtcctaaac ccagagcgtt
     8761 agtggcagtg gacgtgaccg ccaacagcgg acaaaggccg agcaactgga ccagcgcaga
     8821 gttgtttttc cacaacccct gaacaataac gtctttaatt tcgctcacgt tttattctcc
     8881 acaggcagga agttgagaaa gttgtgccgg taacgtctga gcgtacaatc cggcgcgttt
     8941 taccgcatta accaccgcgc ggggagtaat cgtcgcgccg gtgaactggt cgaaatcacc
     9001 accatctttc ttcaccgccc agttcgcatc atccgcacca ctgatttttt tacccgcaaa
     9061 atgggtgatc cagtcagaaa ggcgcagttc gattttatcg ccaagccctg gcgtttcgtg
     9121 gtgctcagtc acgcgcgtgc caagtaccgt gccgttaaaa tcggctccca ccagcagctg
     9181 aatcgcaccg gaatagccat ctggcgcggt tgcttccaga acggcagcta ccggtttgtc
     9241 atcctgtttg gcgatgtaaa cccgatgctc acctttacct aactctggcg cagttaccag
     9301 atagcaactc tgtgccagcg cattgttata gcgttcagct ggcagcacct gatcaaataa
     9361 cgccttttgt tgcagactgg cctgttcagc aattgtcgtt ttggtcatct ggttgatggc
     9421 cgcagttaac cctgttgaac ccgctgcaaa gagcgccaac gtaatgccgt gttttcggat
     9481 agttttcagc atggtttacc ctttgcgatg gccgtagacg cgcggacgcg tgtagtaatc
     9541 gatcagagga accgtgatgt tcgccagcag gacggcaaaa gcaacgccgt caggatagcc
     9601 gccgaaactg cggatcatcc agactaataa gcccgccagc gcgccgaaaa taagacgacc
     9661 acgattggtc gtagaagcgg taaccgggtc agtcaaaata aagaatgcgc cgagcatggt
     9721 cgcaccagac agcagatgaa tttgtggtgc tgccagagta tctggtgaga acaaccagcc
     9781 caacgttgcg cataacgcca gcgttactaa gaagctgagg ggaatatgcc agcgaatcgc
     9841 tttctgccat agcaaccaca cgccgccagc cagccaggcg agatttaccc attgccaacc
     9901 agcgcccgcc agaataccgc tgtagatcgg atattgcata atctcttcaa ccgaatgacc
     9961 ggcacggaca gaggttttaa atgtatccag cggtgtcgcc tgactaatgc catcaatacc
    10021 taagcgtagc gtgttcatat caccaccact ggtggtatga ccactgaaaa taacctgtat
    10081 ggcgtcgata aaaccaggga tattgaccgc aatttcatgt ggcggtaacc aactggtcat
    10141 ctgcaccggg aaggagatca gtaggaccac ataaccaatc attgccggat taaacgggtt
    10201 ttgccccaga ccgccataca actgtttagc gatgatcacc gcaaacaccg tacccagcac
    10261 gaccatccac catggcgcga gagggggaat acttaccgcc agcaataagc ctgtcagcaa
    10321 tgctgagtta tctttcaacg ttgcagctac cgactgcttg cgtagtttga ggacgagagc
    10381 ttcggccaga agagcactga ccgacgccaa caggatctga acgagagtac cccaaccaaa
    10441 aaaccacagt tgcgctgcga ttcctggcac ggctgcgagc aataccaaca gcataatgcg
    10501 cgatgtctgg cgctggttat gggtataagg ggagctagct attctgaata ccatttagtc
    10561 ctcgtttaca accttttgct gggcggcttt tttggcctga acgcgggcaa tagccgcagc
    10621 aactgc
//
LOCUS       DADSYM010000153        10605 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2415
            SAMN08039579-rid11723173.denovo.154, whole genome shotgun sequence.
ACCESSION   DADSYM010000153 DADSYM010000000
VERSION     DADSYM010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039579
            Sequence Read Archive: SRR6362533
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10605)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10605)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980162.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 03:39:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,484
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 4,347
            CDSs (with protein)               :: 4,347
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 43 of 85
            Pseudo Genes (incomplete)         :: 37 of 85
            Pseudo Genes (internal stop)      :: 19 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10605
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039579-rid11723173.denovo.154"
                     /strain="LGE2415"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8302 N 95.9628 W"
                     /collection_date="2015-08-24"
     gene            201..2342
                     /locus_tag="J1Y83_003670"
     CDS             201..2342
                     /locus_tag="J1Y83_003670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent siderophore receptor"
                     /protein_id="HBA7631782.1"
                     /translation="MAMFTPSFSGLKGRALFSLLFAAPMIHATDSVTTKDGETITVTA
                     DANTATEATDGYQPLSTSTATLTDMPMLDIPQVVNTVSDQVLENQNATTLDEALYNVS
                     NVVQTNTLGGTQDAFVRRGFGANRDGSIMTNGLRTVLPRSFNAATERVEVLKGPASTL
                     YGILDPGGLINVVTKRPEKTFHGSVSATSSSFGGGTGQLDITGPIEGTQLAYRLTGEV
                     QDEDYWRNFGKERSTFIAPSLTWFGDNATVTMLYSHRDYKTPFDRGTIFDLTTKQPVN
                     VDRKIRFDEPFNITDGQSDLAQLNAEYHLNSQWTARFDYSYSQDKYSDNQARVTAYDA
                     TTGTLTRRVDATQGSTQRMHATRADLQGNVDIAGFYNEILGGVSYEYYDLLRTDMIRC
                     KKAKDFNIYNPVYGNTSKCTTVSASDSDQTIKQESYSAYAQDALYLTDNWIAVAGIRY
                     QYYTQYAGKGRPFNVNTDSRDEQWTPKLGLVYKLTPSVSLFANYSQTFMPQSSIASYI
                     GDLPPESSNAYEVGAKFELFDGITADIALFDIHKRNVLYTESVGDETIAKTAGRVRSR
                     GVEVDLAGALTENINIIASYGYTDAKVLEDPDYAGKPLPNVPRHTGSLFLTYDIHNMP
                     GNNTLTFGGGGHGVSRRSATNGADYYLPGYFVADAFAAYKMKLQYPVTLQLNVKNLFD
                     KTYYTSSIATNNLGNQIGDPREVQFTVKMEF"
     gene            complement(2406..4298)
                     /gene="parE"
                     /locus_tag="J1Y83_003671"
     CDS             complement(2406..4298)
                     /gene="parE"
                     /locus_tag="J1Y83_003671"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HBA7631783.1"
                     /translation="MTQTYNADAIEVLTGLEPVRRRPGMYTDTTRPNHLGQEVIDNSV
                     DEALAGHAKRVDVILHADQSLEVIDDGRGMPVDIHPEEGVPAVELILCRLHAGGKFSN
                     KNYQFSGGLHGVGISVVNALSKRVEVNVRRDGQIYNIAFENGEKVQDLQVVGTCGKRN
                     TGTSVHFWPDETFFDSPRFSVSRLTHVLKAKAVLCPGVEITFKDEINNTEQRWCYQDG
                     LNDYLAEAVNGLPTLPEKPFIGNFAGDTEAVDWALLWLPEGGELLTESYVNLIPTMQG
                     GTHVNGLRQGLLDAMREFCEYRNILPRGVKLSAEDIWDRCAYVLSVKMQDPQFAGQTK
                     ERLSSRQCAAFVSGVVKDAFILWLNQNVQAAELLAEMAISSAQRRMRAAKKVVRKKLT
                     SGPALPGKLADCTAQDLNRTELFLVEGDSAGGSAKQARDREYQAIMPLKGKILNTWEV
                     SSDEVLASQEVHDISVAIGIDPDSDDLSQLRYGKICILADADSDGLHIATLLCALFVK
                     HFRALVKHGHVYVALPPLYRIDLGKEVYYALTEEEKEGVLEQLKRKKGKPNVQRFKGL
                     GEMNPMQLRETTLDPNTRRLVQLTIDDEDDQRTDAMMDMLLAKKRSEDRRNWLQEKGD
                     MAEIEV"
     gene            complement(4327..4908)
                     /gene="yqiA"
                     /locus_tag="J1Y83_003672"
     CDS             complement(4327..4908)
                     /gene="yqiA"
                     /locus_tag="J1Y83_003672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase YqiA"
                     /protein_id="HBA7631784.1"
                     /translation="MSTLLYLHGFNSSPRSAKASLLKNWLAEHHPDVEMIIPQLPPYP
                     SDAAELLESIVLEHGGDSLGIVGSSLGGYYATWLSQCFMLPAVVVNPAVRPFELLTDY
                     LGQNENPYTGQQYVLESRHIYDLKVMQIDPLEAPDLIWLLQQTGDEVLDYRQAVAYYA
                     SCRQTVIEGGNHAFTGFEDYFNPIVDFLGLHHL"
     gene            complement(4908..5735)
                     /gene="cpdA"
                     /locus_tag="J1Y83_003673"
     CDS             complement(4908..5735)
                     /gene="cpdA"
                     /locus_tag="J1Y83_003673"
                     /EC_number="3.1.4.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3',5'-cyclic-AMP phosphodiesterase"
                     /protein_id="HBA7631785.1"
                     /translation="MESLLTLPLAGEARVRILQITDTHLFAQKHEALLGVNTWESYQA
                     VLEAIRPHQHEFDLIVATGDLAQDQSSAAYQHFAEGIASFRAPCVWLPGNHDFQPAMY
                     SALQDAGISPAKRVFIGEQWQILLLDSQVFGVPHGELSEFQLEWLERKLADAPGRHTL
                     LLLHHHPLPAGCSWLDQHSLRNAGELDTVLAKFPHVKYLLCGHIHQELDLDWNGRRLL
                     ATPSTCVQFKPHCSNFTLDTIAPGWRTLELHADGTLTTEVHRLADARFQPDTASEGY"
     gene            complement(5760..6182)
                     /locus_tag="J1Y83_003674"
     CDS             complement(5760..6182)
                     /locus_tag="J1Y83_003674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1249 family protein"
                     /protein_id="HBA7631786.1"
                     /translation="MKRYTPDFPEMMRLCEMNFSQLRRLLPRNDAPGETVSYQVANAQ
                     YRLTIVESTRYTTLVTIEQTAPAISYWSLPSMTVRLYHDAMVAEVCSSQQIFRFKARY
                     DYPNKKLHQRDEKHQINQFLADWLRYCLAHGAMAIPVY"
     gene            complement(6183..6812)
                     /gene="nudF"
                     /locus_tag="J1Y83_003675"
     CDS             complement(6183..6812)
                     /gene="nudF"
                     /locus_tag="J1Y83_003675"
                     /EC_number="3.6.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribose diphosphatase"
                     /protein_id="HBA7631787.1"
                     /translation="MLKPDNLPVTFGKNDVEIIARETLYRGFFSLDLYRFRHRLFNGQ
                     MSHEVRREIFERGHAAVLLPFDPVRDEVVLIEQIRIAAYDTSKTPWLLEMVAGMIEEG
                     ESVEDVARREAIEEAGLIVKRIKPVLSFLASPGGTSERSSIMVGEVDATTASGIHGLA
                     DENEDIRVHVVSREQAYQWVEEGKIDNAASVIALQWLQLHHQALKNEWA"
     gene            7017..8498
                     /gene="tolC"
                     /locus_tag="J1Y83_003676"
     CDS             7017..8498
                     /gene="tolC"
                     /locus_tag="J1Y83_003676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane channel protein TolC"
                     /protein_id="HBA7631788.1"
                     /translation="MKKLLPILIGLSLSGFSSLSQAENLMQVYQQARLSNPELRKSAA
                     DRDAAFEKINEARSPLLPQLGLGADYTYSNGYRDANGINSNATSASLQLTQSIFDMSK
                     WRALTLQEKAAGIQDVTYQTDQQTLILNTATAYFNVLNAIDVLSYTQAQKEAIYRQLD
                     QTTQRFNVGLVAITDVQNARAQYDTVLANEVTARNNLDNAVEQLRQITGNYYPELAAL
                     NVENFKTDKPQPVNTLLKEAEKRNLSLLQARLSQDLAREQIRQAQDGHLPTLDLTAST
                     GISDTSYSGSKTRGAAGTQYDDSNMGQNKVGLSFSLPIYQGGMVNSQVKQAQYNFVGA
                     SEQLESAHRSVVQTVRSSFNNINASISSINAYKQAVVSAQSSLDAMEAGYSVGTRTIV
                     DVLDATTTLYNAKQELANARYNYLINQLNIKSALGTLNEQDLLALNNALSKPVSTNPE
                     NVAPQTPEQNAIADGYAPDSPAPVVQQTSARTTTSNGHNPFRN"
     gene            8646..9317
                     /locus_tag="J1Y83_003677"
     CDS             8646..9317
                     /locus_tag="J1Y83_003677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417509.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1190 family protein"
                     /protein_id="HBA7631789.1"
                     /translation="MKRTKSIRHASFRKNWSARHLTPVALAVATVFMLAGCEKSDETV
                     SLYQNADDCSAANPGKSAECTTAYNNALKEAERTAPKYATREDCVAEFGEGQCQQAPA
                     QAGMAPENQAQAQQSSGSFWMPLMAGYMMGRLMGGGAGFAQQPLFSSKNPASPAYGKY
                     TDATGKNYGAAQPGRTMTVPKTAMAPKPATTTTVTRGGFGESVAKQSTMQRSATGTSS
                     RSMGG"
     gene            9323..10483
                     /locus_tag="J1Y83_003678"
     CDS             9323..10483
                     /locus_tag="J1Y83_003678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionylspermidine synthase family protein"
                     /protein_id="HBA7631790.1"
                     /translation="MERVSITERPDWREKAHEYGFNFHTMYGEPYWCEDAYYKLTLAQ
                     VEKLEEVTAELHQMCLKVVEKVIASDELMTKFRIPKHTWSFVRQSWLTHQPSLYSRLD
                     LAWDGTGEPKLLENNADTPTSLYEAAFFQWIWLEDQLNAGNLPEGSDQFNSLQEKLID
                     RFVELREQYGFQLLHLTCCRDTVEDRGTIQYLQDCATEAEIATEFLYIDDIGLGEKGQ
                     FTDLQDQVISNLFKLYPWEFMLREMFSTKLEDAGVRWLEPAWKSIISNKALLPLLWEM
                     FPNHPNLLPAYFAEDDHPQMEKYVVKPIFSREGANVSIIENGKTIAAAEGPYGEEGMI
                     VQQFHPLPKFGDSYMLIGSWLVNDQPAGIGIREDRALITQDMSRFYPHIFVE"
BASE COUNT         2691 a         2803 c         2597 g         2514 t
ORIGIN      
        1 gcaataaaat tgtataaacc tttcataaaa acacatgtag caattttttg ttatttctca
       61 tttactacta cattactgcg tgattgaatg attctcaatt acatatccga tggcgaaata
      121 acaaacggat agtgcttagt gagcaataag aaacaatttt cactcgcgac aatccgcgag
      181 tttttgggat aacagactaa atggctatgt tcacaccttc attctcagga ctcaaaggtc
      241 gggcgctctt ttcactgctt tttgcggcac cgatgattca tgcaacagac tctgtaacga
      301 ccaaagatgg cgaaacaatc actgttacag cagatgcaaa taccgcaact gaggcaaccg
      361 atggttatca acctctgagc acctctacgg cgacattaac cgatatgccg atgctggata
      421 tcccgcaggt ggtcaatacg gttagcgatc aggttctgga aaaccagaat gcgacaacgc
      481 tggatgaggc gctttataac gtcagtaacg tggtacagac caatacatta ggcgggactc
      541 aggatgcttt tgtacgccgt ggatttggcg caaaccggga tggctccatc atgaccaacg
      601 gtctgcgaac cgtacttcct cgtagtttca acgccgcaac agagcgtgtg gaagtgctaa
      661 aaggcccggc ctccacgctg tatggcattc tcgatcctgg cggactgatt aacgtcgtga
      721 ccaagcgccc ggaaaaaaca ttccatggtt cggtttcagc cacctcctcc agttttggtg
      781 gcggcactgg gcaacttgat atcacaggtc ccattgaagg cactcagctg gcgtatcgtc
      841 ttaccgggga agtgcaggat gaagattact ggcgaaactt cggtaaagag cgcagtacat
      901 ttattgcccc gtcactcacc tggtttggtg ataatgcaac agtaaccatg ctctattccc
      961 atcgggacta taaaactccg ttcgatcgtg gaacgatttt cgaccttacg acgaaacagc
     1021 ccgtaaacgt tgatcgaaaa atacgttttg acgaaccgtt taatattaca gatggtcagt
     1081 ccgatctggc gcaactcaac gcagaatatc atctcaatag ccagtggaca gcgcgctttg
     1141 attacagcta cagccaggat aaatacagcg ataatcaggc gcgtgttacc gcgtatgatg
     1201 caacgacagg aacactgaca cggcgtgttg atgcaactca gggatctacc cagcgtatgc
     1261 atgctactcg tgcggatctg caagggaatg ttgatattgc cggattctat aatgagattc
     1321 tgggtggggt gtcatatgaa tattatgatc ttctgcgtac agatatgatt cgctgtaaaa
     1381 aagctaaaga tttcaatata tacaaccctg tttatggtaa taccagcaaa tgtacaacgg
     1441 tttcggcgtc ggacagtgat cagacgatca aacaggagag ctactcagcg tatgcacagg
     1501 acgcactcta tctgaccgat aactggattg ccgtcgccgg gatccgctat cagtattaca
     1561 cgcagtatgc gggtaaaggc cgtcctttta atgtcaatac tgacagccgc gatgaacaat
     1621 ggactcccaa actggggttg gtctacaaac tgacgccatc ggtatcctta tttgccaatt
     1681 attcgcaaac atttatgcca cagtcgtcaa ttgccagcta cattggcgat cttccaccag
     1741 aatcatctaa tgcttacgaa gtaggggcaa aattcgagct attcgatggt atcaccgcag
     1801 atattgcgct gtttgatatc cataaacgta atgtgttgta taccgaaagt gttggtgatg
     1861 aaaccattgc caaaacggca ggccgtgttc gttcaagagg ggtagaagtc gaccttgcgg
     1921 gagcattaac tgaaaacatt aatatcattg ccagctacgg ctataccgat gctaaggttc
     1981 tggaagatcc tgattatgca ggtaaaccat tgccgaatgt tcctcgtcat accggttcgc
     2041 tattcctgac ctatgacatt cataacatgc ctggcaataa cacactgacg tttggcggtg
     2101 gtggacatgg cgtaagccgt cgttcggcaa ccaatggggc tgactattat ctgccaggct
     2161 attttgttgc cgatgccttc gccgcataca aaatgaaatt gcagtatccg gtcacactgc
     2221 aattaaacgt caaaaacctg tttgataaaa cgtattacac ctcttccatc gccacaaata
     2281 atctgggcaa ccagattggc gatccgcgtg aagtgcaatt cacggtgaaa atggaatttt
     2341 gatcaaaaaa gcccggtata cacttgtata ccgggctatg ttttattctt ctggcgggag
     2401 gattatcaga cctcaatctc cgccatgtcg cctttttctt gcaaccagtt gcggcgatct
     2461 tccgagcgtt tcttcgccag cagcatatcc atcatcgcgt cagtacgctg atcgtcttca
     2521 tcatcgatag tcaactgcac cagacggcga gtgttcggat caagcgtggt ttcgcgcaat
     2581 tgcatcgggt tcatttcccc gagaccttta aaacgctgga cgtttggctt gcctttcttg
     2641 cgttttaatt gctcaagtac gccctctttc tcttcttccg tcagcgcgta ataaacctct
     2701 ttcccgaggt caatacggta gagcggtggc agtgcaacgt aaacgtgacc gtgtttcacc
     2761 aatgcgcgga agtgttttac aaacaaagcg cagagcagcg tggcaatgtg cagaccatcg
     2821 gagtcagcat ccgccaggat acagatcttg ccgtaacgaa gctggctcaa atcgtcgctg
     2881 tcaggatcga taccgatagc taccgaaata tcgtgcactt cctgcgaagc cagcacttcg
     2941 tcggaagaga cttcccaggt attaaggatc ttacctttca gtggcatgat cgcctgatat
     3001 tcgcgatcgc gcgcctgctt ggcagatccg cctgcggagt caccttccac aaggaatagt
     3061 tcggtacggt taagatcctg cgcggtacag tcagccaatt taccaggcag cgccgggccg
     3121 ctggttagct ttttacgcac cactttttta gccgcacgca tacgacgctg ggcgctggaa
     3181 atcgccatct ctgccaacag ctccgccgcc tgaacgttct ggttcagcca caggatgaag
     3241 gcatctttca ccacgcccga aacgaatgcc gcacactgac gcgaagagag acgttctttg
     3301 gtctgcccgg caaactgcgg atcctgcatt tttactgaca gcacataggc gcagcgatcc
     3361 cagatatctt ccgccgacag ctttacaccg cgcggcagaa tgttgcggta ttcacagaac
     3421 tcacgcatcg cgtccagcag gccctgacgc aggccattaa catgggtacc gccctgcatc
     3481 gttgggatca ggttgacgta gctttcggtc agcagttcac cgccttccgg cagccacagt
     3541 agcgcccagt ccaccgcttc agtatcgcca gcgaaattac cgataaacgg tttttctggc
     3601 agcgtcggta aaccgtttac cgcttccgcc aggtaatcat tcagaccgtc ctgatagcac
     3661 cagcgctgtt cggtgttgtt gatctcatct ttaaaagtga tctcaacgcc cgggcacagt
     3721 accgctttgg ctttcagcac atgcgtcagg cgtgaaacag aaaaacgcgg gctgtcaaag
     3781 aaggtttcat ccggccagaa gtgcacgctg gtaccggtat tgcgtttacc gcaagtgccg
     3841 acaacctgta aatcttgcac cttttcgcca ttttcaaagg cgatgttata gatctgacca
     3901 tcgcggcgta cgttaacttc tacgcgcttc gacagggcgt taactaccga aatccccacg
     3961 ccatgcaggc cgccagagaa ctggtaattt ttgttagaga atttaccgcc cgcgtgcaga
     4021 cggcaaagaa tcagttcaac cgccggtacc ccctcttccg ggtgaatatc taccggcatc
     4081 ccgcgcccat cgtcaatcac ttctaacgac tggtcggcat gtaagattac atccacgcgt
     4141 tttgcgtggc ccgccagtgc ttcatcgaca ctgttatcaa taacttcttg ccccaaatgg
     4201 ttagggcgag tggtatcggt atacatcccc ggacggcggc gaaccggctc aagcccggtg
     4261 agtacctcaa tggcatcagc gttataagtt tgcgtcatgg tttaagttag caattcgagt
     4321 tgatcgtcag agatggtgca gaccaagaaa atcgacgatc gggttgaaat aatcttcgaa
     4381 gcccgtgaat gcgtggttgc cgccttctat gacagtctgg cggcaggaag cgtagtacgc
     4441 caccgcctgg cggtaatcca gcacttcatc tcccgtctgt tgcagcagcc agatcaaatc
     4501 cggcgcttcc agcgggtcaa tctgcatgac tttgagatcg taaatatggc gtgactctag
     4561 cacatattgc tgcccggtgt aggggttctc gttctgaccg agatagtccg tcagcagttc
     4621 aaacgggcgc accgccgggt tgaccaccac tgcgggcagc ataaaacatt gcgacaacca
     4681 ggtggcgtaa tatcccccca gtgacgaacc gacaataccc agcgaatcgc cgccatgttc
     4741 caggacaatg gattccagca gttctgccgc gtcagaagga tacggcggca actgcggaat
     4801 gatcatctca acgtcagggt gatgttccgc cagccagttt tttaacaagc tcgcttttgc
     4861 agagcgcggc gagctgttga aaccgtgtaa ataaagaagc gtagacatca gtagccttct
     4921 gaagcggtat caggttggaa gcgtgcgtcc gccaggcgat gcacctcggt ggtcagcgtg
     4981 ccatcagcat gtaactcgag agtacgccag ccgggcgcga tggtgtccag cgtaaagtta
     5041 gaacagtgcg gcttaaactg cacacaggtc gacggcgttg ccagcaagcg gcgaccgttc
     5101 cagtcgagat ccagctcctg atgaatgtga ccgcacagca aatatttgac gtgcggaaac
     5161 ttcgccagca cggtatccag ttcgcccgcg ttacgcaggc tgtgttgatc aagccaacta
     5221 caacctgcag gtagcggatg atgatgcagc agcagcaacg tatggcgtcc tggcgcatcg
     5281 gccagtttac gttccagcca ctcaagctga aactcgctca gctcaccgtg cggcacgcca
     5341 aacacctggc tatccagcaa caggatttgc cattgctcac caataaacac gcgcttcgcc
     5401 ggggagatac ccgcatcctg taacgcactg tacatcgcgg gctggaaatc gtggttgccc
     5461 ggcagccaga cgcagggcgc acgaaaactt gcgatgcctt cagcgaaatg ctgataggcc
     5521 gcagaggatt gatcctgcgc taaatcacct gtcgcgacaa tcaggtcgaa ttcgtgctgg
     5581 tgtggccgaa tcgcctccag caccgcctgg tagctttccc aggtgtttac ccctaacagg
     5641 gcttcgtgct tttgtgcaaa caggtgagtg tcggtaattt gtaaaatcct gactctggcc
     5701 tcaccagcca gaggaagggt taacaggctt tccaaatggt gtccttaggt ttcacgacgc
     5761 taataaaccg gaatcgccat cgctccatgt gctaaacagt atcgcaacca gtccgctaaa
     5821 aactgattaa tttgatgctt ttcgtcgcgt tgatgcaact ttttattagg ataatcatac
     5881 cgcgctttga agcgaaaaat ctgctggctt gaacacactt cagccaccat cgcgtcatga
     5941 tacagacgca ccgtcattga cggaaggctc cagtaactga tcgcgggcgc agtctgttct
     6001 attgtcacca gggtagtgta tcgggtcgat tccacaatcg tcagccgata ttgtgcgttt
     6061 gccacctgat agcttacagt ttcgccgggt gcgtcattgc gcggtaacaa acggcgcaat
     6121 tgtgaaaagt tcatctcgca caggcgcatc atttcaggaa agtcaggtgt gtaacgcttc
     6181 atttatgccc actcattttt taacgcttga tgatgcagct gcagccattg caaagcgatg
     6241 accgacgctg cgttgtcgat tttcccctct tctacccact ggtatgcctg ttcccggctt
     6301 accacatgaa cgcgaatatc ttcgttttca tcagccagac cgtgaatacc gcttgcggtc
     6361 gtggcgtcca cttcgcccac cataattgac gaacgctcac tggtgccccc cgggcttgcc
     6421 aggaaactta acaccggttt gatccgtttg actatcagtc ccgcctcttc aatcgcttcg
     6481 cgacgggcaa catcttccac actttcaccc tcttcaatca tcccggcaac catctccagt
     6541 agccaggggg ttttgctggt gtcgtacgcg gcaatccgaa tctgctcaat cagcacaact
     6601 tcatcacgca ctgggtcaaa gggtagcaag actgcggcgt gaccgcgctc aaaaatttcc
     6661 cgccgtacct catgactcat ttgcccgttg aatagacgat gacgaaatct ataaagatct
     6721 aatgaaaaaa agccgcgata aagtgtttct cgtgcaataa tttctacatc gtttttgcca
     6781 aatgtaacgg gcaggttgtc tggcttaagc attgttaatg tcctggcact aatagtgaat
     6841 taaatgtgaa tttcagcgac gtttgactgc cgtttgagca gtcatgtgtt aaattgaggc
     6901 acattaacgc cctatggcac gtaacgccaa ccttttgcgg tagcggcttc tgctagaatc
     6961 cgcaataatt ttacagtttg atcgcgctaa atactgcttc accacaagga atgcaaatga
     7021 agaaattgct ccccattctt atcggcctga gcctctctgg gttcagttcg ttgagccagg
     7081 ccgagaacct gatgcaagtt tatcagcaag cacgccttag taacccggaa ttgcgtaagt
     7141 ctgccgccga tcgtgatgct gcctttgaaa aaattaatga agcgcgcagt ccattactgc
     7201 cacagctagg tttaggtgca gattacacct atagcaacgg ctaccgcgac gcgaacggca
     7261 tcaactctaa cgcgaccagt gcgtccctgc agttaactca atccattttt gatatgtcga
     7321 aatggcgtgc gttaacgctg caggaaaaag cagctgggat tcaggacgtc acgtatcaga
     7381 ccgatcaaca aaccttgatc ctcaacaccg cgaccgctta tttcaacgtg ttgaatgcga
     7441 ttgacgttct ttcctataca caggcgcaaa aagaagcgat ctaccgtcaa ttagatcaaa
     7501 ccacccaacg ttttaacgtg ggcctggtag cgatcaccga cgtgcagaac gcccgcgcac
     7561 agtacgatac cgtgctggcg aacgaagtga ccgcacgtaa taaccttgat aacgcggtag
     7621 agcagctgcg ccagatcacc ggtaactact atccggaact ggcggcgctg aatgtcgaaa
     7681 actttaaaac cgacaaacca cagccggtta acacgctgct gaaagaagcc gaaaaacgca
     7741 acctgtcgtt gttacaggcg cgcttgagcc aggacctggc gcgcgagcaa attcgccagg
     7801 cgcaggatgg tcacttaccg actctggatt tgacggcctc taccggaatt tctgacacct
     7861 cttacagcgg ttcaaaaacc cgtggtgccg ctggtaccca gtatgacgat agcaatatgg
     7921 gccagaacaa agttggcctg agcttctcgc tgccgattta tcagggcgga atggttaact
     7981 cgcaggtgaa acaggcccag tacaactttg ttggtgccag cgagcaactg gaaagcgcgc
     8041 atcgtagcgt cgtacaaacc gtacgttcct ccttcaacaa cattaatgct tctatcagta
     8101 gcattaacgc ctacaaacaa gccgtagttt ccgctcaaag ttcattagac gcgatggagg
     8161 cgggttactc ggtcggtacg cgtaccattg ttgatgtgtt ggatgcgacc accacgctgt
     8221 acaacgctaa gcaagagctg gcaaatgcgc gttataacta cctgattaac cagttgaata
     8281 ttaagtcagc tctgggtacg ttgaacgagc aggatctgct ggcactgaac aatgcgctga
     8341 gcaaaccggt ttccactaat ccggaaaacg ttgccccgca aacgccggaa cagaatgcta
     8401 ttgctgatgg ttatgcgcct gatagcccgg caccagtcgt tcagcaaaca tccgcacgca
     8461 ctaccaccag taacggtcat aaccctttcc gtaactgatg acgacgacgg ggcttcggcc
     8521 ccgtctgaac gtaaggcaac gtaaagatac gggttatctg ccgcattctt cccccttctc
     8581 gcttcaattt cgaccagcca tcctctattc tgatgggtat ttaccactgg tcccggaaga
     8641 caaaaatgaa acggacaaaa tccatacgcc acgcatcgtt ccgcaaaaac tggagcgcac
     8701 gccatctgac accagtcgct ctcgcggttg ccactgtttt tatgctggcg ggctgtgaaa
     8761 agagtgatga aacagtgtct ctctatcaaa atgctgacga ctgttcagct gcaaacccag
     8821 gcaaaagcgc cgaatgtacc accgcgtaca acaatgcgct gaaagaagcc gaacgtactg
     8881 cgccgaaata cgccacccgt gaagactgtg ttgctgaatt tggtgaaggt cagtgccagc
     8941 aggcaccagc ccaggctggc atggcaccag aaaaccaggc gcaggcccag caatccagcg
     9001 ggagtttctg gatgccgctg atggccggtt acatgatggg acgtctgatg ggcggcggcg
     9061 cgggatttgc acagcagccg ctgttctcct cgaaaaaccc ggccagcccg gcttacggta
     9121 aatacaccga cgcgaccggt aaaaactatg gcgcagccca gccaggccgc accatgaccg
     9181 taccgaagac ggcaatggca ccaaaaccgg cgaccactac tactgttacc cgtggcggtt
     9241 ttggtgaatc tgttgccaaa caaagcacta tgcagcgtag cgcaaccggc acctcttctc
     9301 gttcaatggg tggctgatac cgatggaaag agtcagtatt accgagcgcc cggactggcg
     9361 cgaaaaagcc cacgaatacg gtttcaattt tcacaccatg tacggcgagc cgtactggtg
     9421 tgaagatgct tactacaagt tgaccctcgc ccaggttgaa aagctggaag aagtcaccgc
     9481 cgaactgcac cagatgtgcc tgaaagtggt ggaaaaagtg atcgccagcg atgagctgat
     9541 gaccaaattc cgcattccaa aacacacctg gagttttgtg cgccagtcat ggctgacgca
     9601 ccagccatcg ctttattcgc gtcttgatct ggcatgggat ggtactggcg aacctaaact
     9661 tctggaaaat aacgccgata cgccaacgtc actatacgag gcggcgttct ttcagtggat
     9721 ctggctggaa gatcagctta acgccggtaa cttgccggag ggcagcgacc agtttaacag
     9781 tttgcaagaa aagctgatcg atcgcttcgt tgagctgcgt gaacagtatg gcttccagtt
     9841 gctgcatctc acctgctgtc gcgacacggt ggaagatcgc ggaaccattc agtatttgca
     9901 ggactgcgcg acggaagctg aaatcgctac cgagttcctc tacatcgacg atatcgggtt
     9961 aggtgaaaaa ggtcagttca cggatttaca ggatcaggtg atttccaacc tgttcaagct
    10021 gtatccgtgg gaatttatgt tgcgtgagat gttctccacc aagctggagg atgcaggcgt
    10081 acgctggctg gaaccggcgt ggaagagcat tatctccaac aaagcgcttc taccgctact
    10141 gtgggagatg ttcccgaatc acccgaacct gctgcccgct tattttgcgg aagatgatca
    10201 tccgcaaatg gaaaaatatg tggttaaacc gatcttctcc cgtgaaggcg caaacgtgtc
    10261 gatcattgag aacggcaaaa ccattgcagc agcggaaggt ccgtatggcg aagaagggat
    10321 gattgttcag caattccacc cgttaccgaa attcggcgac agctatatgc tgattggtag
    10381 ctggctggtg aacgatcaac ctgccggaat tggcattcgt gaagaccgtg cattgatcac
    10441 ccaggatatg tctcggtttt atccgcatat ttttgttgag taatacagct taatattgcc
    10501 ccgataagcg gggcaatctc ttataatcca ccattcataa tttccatcaa tatataaaca
    10561 catacatatc caacctttat ttaacattaa tactcaattt aatgt
//
LOCUS       DADSYM010000154        10391 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2415
            SAMN08039579-rid11723173.denovo.155, whole genome shotgun sequence.
ACCESSION   DADSYM010000154 DADSYM010000000
VERSION     DADSYM010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039579
            Sequence Read Archive: SRR6362533
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10391)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10391)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980162.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 03:39:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,484
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 4,347
            CDSs (with protein)               :: 4,347
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 43 of 85
            Pseudo Genes (incomplete)         :: 37 of 85
            Pseudo Genes (internal stop)      :: 19 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10391
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039579-rid11723173.denovo.155"
                     /strain="LGE2415"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8302 N 95.9628 W"
                     /collection_date="2015-08-24"
     gene            153..962
                     /gene="pduF"
                     /locus_tag="J1Y83_003679"
     CDS             153..962
                     /gene="pduF"
                     /locus_tag="J1Y83_003679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004016432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propanediol diffusion facilitator PduF"
                     /protein_id="HBA7631791.1"
                     /translation="MNDSLKAQCVAEFLGTGLFLFFGIGCLCALKVAGASLGLWEICI
                     IWGLGISLAVYLTAGISGAHLNPAVTIALWLFASFPARKVLPFCVAQLAGAFGGAVLA
                     YSLYSSLFTDFESAHNIVRGSAESLQLASIFSTYPAAAINVWQAALVEVVITSMLMGL
                     IMALTDDGNGVSKGPLAPLLIGILVAVIGASTGPLTGFAMNPARDFGPKIFTWFAGWG
                     EIAMTGGRNIPYFIVPIIAPIIGACVGAAIYRYLIAKNLPVNSVTPKEISE"
     gene            1165..2078
                     /gene="pocR"
                     /locus_tag="J1Y83_003680"
                     /pseudo
     CDS             1165..2078
                     /gene="pocR"
                     /locus_tag="J1Y83_003680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460981.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory protein PocR"
     regulatory      2170..2345
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            2563..3000
                     /locus_tag="J1Y83_003681"
     CDS             2563..3000
                     /locus_tag="J1Y83_003681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001524956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin biosynthesis protein"
                     /protein_id="HBA7631792.1"
                     /translation="MKTIKPESIALFCLLSGDVDLAKHLAEILPITCLVGEDWQQDFQ
                     PFDGGPVQSLSDAYSDYSVLLLIAAESEISVPEWLAHSEGTTVILIAEHNARLLNGPA
                     GGAVALIHYLNEICHARETAVNDAFCRTLTRGSLAIPQCHLLS"
     gene            3134..3679
                     /gene="cobU"
                     /locus_tag="J1Y83_003682"
     CDS             3134..3679
                     /gene="cobU"
                     /locus_tag="J1Y83_003682"
                     /EC_number="2.7.1.156"
                     /EC_number="2.7.7.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional adenosylcobinamide
                     kinase/adenosylcobinamide-phosphate guanylyltransferase"
                     /protein_id="HBA7631793.1"
                     /translation="MMILVTGGARSGKSRHAEALIGDSSQVLYIATSQILDDEMAARI
                     EHHRQGRPEHWRTVERWQHLDELIHADINPNEAVLLECVTTMVTNLLFDYGGDKDPDE
                     WDYQAMEQAINAEIQSLIAACQRCPAKVVLVTNEVGMGIVPESRLARHFRDIAGRVNQ
                     QLAAAANEVWLVVSGIGVKIK"
     gene            3676..4419
                     /gene="cobS"
                     /locus_tag="J1Y83_003683"
     CDS             3676..4419
                     /gene="cobS"
                     /locus_tag="J1Y83_003683"
                     /EC_number="2.7.8.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide-GDP ribazoletransferase"
                     /protein_id="HBA7631794.1"
                     /translation="MSKLFWAMLSFITRLPVPRRWSQGLDFEHYSRGIITFPLIGLLL
                     GAISGLVFMVLQAWCGVPLAALFSVLVLALMTGGFHLDGLADTCDGVFSARSRDRMLE
                     IMRDSRLGTHGGLALIFVVLAKILVLSELTLRGEPILASLAAACAVSRGTAALLMYRH
                     RYAREEGLGNVFIGKIDGRQTCVTLGLAAIFAAVLLPGMHGVAAMVVTMVVIFILGQL
                     LKRTLGGQTGDTLGAAIELGELVFLLALL"
     gene            4431..5510
                     /gene="cobT"
                     /locus_tag="J1Y83_003684"
     CDS             4431..5510
                     /gene="cobT"
                     /locus_tag="J1Y83_003684"
                     /EC_number="2.4.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide--dimethylbenzimidazole
                     phosphoribosyltransferase"
                     /protein_id="HBA7631795.1"
                     /translation="MQILADLLNTIPAIDPAAMSRAQRHIDGLLKPVGSLGKLEALAI
                     QLAGMPGLNGIPHVGKKAILVMCADHGVWEEGVAISPKEVTAIQAENMTRGTTGVCVL
                     AAQAGANVHVIDVGIDTAEPIPGLINMRVARGSGNIASAPAMSRRQAEKLLLDVICYT
                     RELAKNGVTLFGVGELGMANTTPAAAIVSTITGRDPEEVVGIGANLPTDKLANKIDVV
                     RRAITLNQPNPRDGVDVLAKVGGFDLVGIAGVMLGAASCGLPVLLDGFLSYAAALAAC
                     QMSPAIKPYLIPSHLSAEKGARIALSHLGLEPYLNMDMRLGEGSGAALAMPIIEAACA
                     IYNNMGELAASNIVLPGNTTSDLNS"
     gene            5572..6498
                     /gene="ldtA"
                     /locus_tag="J1Y83_003685"
     CDS             5572..6498
                     /gene="ldtA"
                     /locus_tag="J1Y83_003685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001773987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HBA7631796.1"
                     /translation="MMRRVNILCSFALLFASQNSLAVTYPLPPEGSRLVGQSLTVTVP
                     DHNTQPLETFAAQYGQGLSNMLEANPGADVFLPKSGSQLTIPQQLILPATVRKGIVVN
                     VAEMRLYYYPPDSNTVEVFPIGIGQAGRETPRNWVTTVERKQEAPTWTPTPNTRREYA
                     KRGESLPAFVPAGPDNPMGLYAIYIGRLYAIHGTNANFGIGLRVSQGCIRLRNDDIKY
                     LFDNVPVGTRVQVIDQPVKYTMEPDGSKWLEVHEPLSRNRAEYESDRKVPLPVTPSLR
                     AFINGQEVDVNRANAALQRRSGKPVNINRNGF"
     gene            6672..7637
                     /locus_tag="J1Y83_003686"
     CDS             6672..7637
                     /locus_tag="J1Y83_003686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001524950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA7631797.1"
                     /translation="MTKQNKNSRWQGYDPRYIYSLVVRRYFADMKTKIEIAEELGVSR
                     FKVARLIDEAIEQDYVRFIFPKQQAMDEEIANNLRKKFHLEDAIVLSVAESWTTQEEL
                     NHKLGEVTAEYLMQSLHEGMKVGIAWGRVLSSTVSKLSKLPPLDVVQLSGVHPGIEFS
                     QGPIDLIHKIAAISQGKAHPMYVPMWVDDEELAARLAGDPAVLDTQQYYSQLDVVITG
                     IGDWKSGSSSLCKIFPDTWCEALFQQDIAADVCISLVSREGKILHSPIERLGFGISTD
                     QLQKAKKVIGVAGGEEKYEGILASLKSGLLNVLITDFDTAIKLLD"
     gene            complement(7689..8147)
                     /locus_tag="J1Y83_003687"
     CDS             complement(7689..8147)
                     /locus_tag="J1Y83_003687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001683480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBA7631798.1"
                     /translation="MKFEILVNDVDNSISDYQTAITFAGTQLLEKGYITAEYIDACLE
                     REKSYPTGLMMANGQGIAIPHADYTLVNVNSISIVRFANDVTFGQMEDADLTVDCSIM
                     FNLAFATSDQHMSILRRLFTLFQDNAFVETCRNLPATRVAKYVEDTLAAS"
     gene            complement(8158..8442)
                     /locus_tag="J1Y83_003688"
     CDS             complement(8158..8442)
                     /locus_tag="J1Y83_003688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014172369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBA7631799.1"
                     /translation="MKKILVVCGNGIASSSIMVSVLQDFLKEEKIEAQVDKSSLMACT
                     SDTFNSYDLVVSSTKLDNPAITTRVIVGAGLLTGLGEDEIFAAVKEEMLK"
     gene            complement(8466..9836)
                     /locus_tag="J1Y83_003689"
     CDS             complement(8466..9836)
                     /locus_tag="J1Y83_003689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001564759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HBA7631800.1"
                     /translation="MELITQFINDLGNFIFIPVIFLVLMKILGRPLSECISSAIKVGI
                     GFIALTMTIKLMLEKMQPAVTGLAEATGSSLSAIDVGGAATAVMGFGSSMGAIIIPLC
                     VAVNIAMLVARLTDCVNVDVFNLHQNASMGAIVGVYSGSFLYGALTAALFHVWALIAA
                     DLGAKNNEKFFNLPKGVAISHPVANTYLLFAYPFNWIYDRIPGFRNLNVTAETIQKRF
                     GILGDPTMVGFIIGILLGFCGYGWKSPYHTIIASLQLGMYLAAVMLLLPRMTSIMMEG
                     LVPLSNVARKKLVKRFPDRQITVGMDTALIVGHPSVIAPALLLIPVIVILAVILPGNR
                     VMPLGDLSQFVFFIACMVPVFNGNIIRTWVTSIILFGGGLYIASWMAPATNEVFQKFG
                     TNPDASVMYSSLNPSANPFTGLFAALSHVGIIGYVMAGILLLSIGYLIKQKSRRQIET
                     DLEKAL"
     gene            complement(9840..10376)
                     /locus_tag="J1Y83_003690"
     CDS             complement(9840..10376)
                     /locus_tag="J1Y83_003690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000850105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RpiB/LacA/LacB family sugar-phosphate isomerase"
                     /protein_id="HBA7631801.1"
                     /translation="MKTKIAIASDDVGFARKEEIKKYLIEEKNMEIVYDPVICKEQGV
                     NNFARLADEMAGIIQRDECRLGIYICGTGIGFTCQINKHWGIRAVAVTNPYSAKRARL
                     SNNAQVIGLGCRVNDLEYSKMIVDAWLDNPFDFTTARENSKKNLLEAERNDNALLVKP
                     ADVRWNMGFRPDDEKSEG"
BASE COUNT         2651 a         2233 c         2650 g         2857 t
ORIGIN      
        1 taatttgtta tgttagcacg atttttacac gagtctgtca tgcatgttgg caatattttc
       61 tgcagagcaa ttttttatag ttgcgctcgg tatacacaac aatcagcgca tagttggctg
      121 ataaagaaaa actccgtaaa gaaggtgtca ctatgaatga ttcactcaag gcgcaatgcg
      181 ttgccgagtt tttgggcacc ggactgttcc tcttttttgg cattggttgt ttgtgcgccc
      241 tgaaggttgc cggggcaagt ctgggactgt gggaaatttg tatcatttgg ggattaggga
      301 tttcgcttgc tgtttacctt acggcaggta tttccggcgc tcatctcaac ccggcagtta
      361 ccattgctct gtggctgttt gcctctttcc cagcccgtaa agtgctgcca ttttgtgtgg
      421 cgcaattagc gggtgccttt ggtggtgcgg ttctggcata ttctctctac agcagtttgt
      481 ttaccgattt tgaatctgct cacaatatag tgcgtggtag cgcagaaagt ttacaactcg
      541 ccagtatctt cagtacttat ccagcagcgg cgattaatgt atggcaagct gcgttggtcg
      601 aagtggtcat aacgtccatg ttaatggggt tgattatggc gttgaccgat gacggtaatg
      661 gcgtatccaa aggaccgctt gcgcctttac tcattggtat tctggttgct gttattggtg
      721 cttctaccgg accattaacc ggttttgcca tgaatccagc gcgtgatttt ggacctaaaa
      781 ttttcacctg gtttgcgggt tggggggaaa ttgcgatgac tggtggacgc aatattcctt
      841 atttcattgt acccattatt gcaccgatca ttggggcatg tgttggtgcg gcgatttatc
      901 gctatctcat tgcaaagaat ttacctgtca atagtgtaac gcctaaagaa atcagcgaat
      961 aacgaaataa tctggcagtt ataaacgttt atttttttac tgtttgtgat ctgctttccg
     1021 aacttattaa aaagtctccc gcttcacgga ggcttttgtt attgtccttt gtgcttctcg
     1081 tcagtaaatc atcaaggatg atgtgaagat taaatgtaaa acaattgttt tgctataaca
     1141 ataaattaac tgggaggttt tatcatgatt tctgcggcga cacttaattc agaactcatt
     1201 aataaaatcg cgcaggattt tgcgcaggcg accagccttg cggttgtcgt ggtcaatatt
     1261 cacggcgatg aaatttccga gctgtttaac ttcactccat tttgtcagat gatgcggcaa
     1321 caccctcagc tgagcactcg atgtcgcatg aacgatcgtt gtggggtctg gaagcctcta
     1381 aatccgatca gccttgtatt tatcgctgtc atgccggatt aaccgatttc tctattccac
     1441 tggtaatcgc cgggcattta gttggttttg tattgtgcgg gcaagttcgt ctgcgtaatg
     1501 atgatgtcga tttggtcgat attctgaatg tcgatgattg ctggcaggca gatcccgaat
     1561 tacttaatga atttcgcaaa gtccctgaga tggattactc aagagttatg gcctctgcgg
     1621 atttgttgaa gttgattgta gaaaattgcc taaaaaaaca actcaatttt gtggttatta
     1681 aagagaacaa atctcagtca gattcgttgc gccagactcg tgcgcctggt ccccacgaca
     1741 ataaaatgaa aaaagcttta cgttatattg atgcgcattt atcagacgat ttgcgtctgg
     1801 aagatgtcgc ctcgcacgtt tatctaagcc cttattattt cagtaagtta tttaagaaat
     1861 accatgggat cgggtttaat gcgtgggtaa accaacaacg gatggccagc gctcgtgagt
     1921 tactgtgcca tagtgactgg agcatcgcca gcattgcgcg taatttaggt ttttcgcaaa
     1981 caagttattt ttgcaaagtt ttccgtcaga cttatcaggt tacgccgcag gtgtatcgac
     2041 agcaagtgag tgataatgcg gcgatggaaa cggtatagag taaatgcccc aaaaaaagag
     2101 gacggtttaa ataaactctg gaatgaataa ttaacgttat ttccctgtta tgggaaaagt
     2161 gaaatcagct cataacatac gctgacaggt gtggcacatt tgtgcctggg aagagggtga
     2221 aaatccctcg cagcccccgc tgctgtaatg ctgacaaacc cgaaaatgcc actgatcgcg
     2281 agattgggaa ggtcgggaga ggatgacgct aagccagaag accagccagt cagtcaatac
     2341 caacaacttc ggtgggaagt gggttgcccg gaagcgtccg gtagagtaca ggcgcgtctt
     2401 tataccccta ccaccctgaa aggatcaggg tatggcggca tcatttagtt cttttatcgc
     2461 agtgccgaca ggcaccaggg ctgcacttaa ggtgtggcaa tcttgtttca tggcttatca
     2521 accagaccgc cattacagga aattaagtgg agggcgaggg gcatgaaaac gataaaaccc
     2581 gaatcgatag ctcttttttg cctcttatct ggagatgtgg atctggcgaa acatctggcg
     2641 gaaatattgc caataacctg tttggttggt gaggactggc agcaagattt ccagcctttt
     2701 gacggtgggc ctgtgcaatc gttatcagat gcgtatagcg attattcagt gctgctattg
     2761 atagcagctg aaagcgagat atccgtacca gaatggctcg ctcattctga gggaacgacg
     2821 gtaattttga ttgcagaaca caatgcccgt ttgctgaatg gtccggctgg tggggctgta
     2881 gcattaatcc attatctgaa tgagatctgt catgccagag aaaccgctgt caatgacgca
     2941 ttttgtcgga cgttaacacg aggaagtctg gctattccgc aatgccatct tctttcgtaa
     3001 tggcttgaga atgctggatt tttaattata ttacgcgcag tattaatcgc gtaaatgttt
     3061 tgtggtcagc aacggtgggg gatccattgt tctgttcaat gaataccatc atacatttga
     3121 atcaggagtc ggtatgatga ttttggtgac ggggggcgca cggagcggga agagtcgcca
     3181 tgcagaggcg cttattgggg actcttcaca ggttctgtat atcgctacct cgcaaatcct
     3241 tgatgatgag atggctgcac ggatagaaca tcatcggcaa ggccgcccgg agcactggcg
     3301 cacagtggag cgctggcaac atcttgatga attaattcat gcagacatta acccgaatga
     3361 ggctgtgttg ctggaatgcg ttaccacaat ggtgactaat ctgttgtttg attatggcgg
     3421 cgataaagac cctgatgaat gggattatca ggcgatggaa caggcgatta atgctgagat
     3481 tcagtcgttg attgctgcct gccaacgttg ccccgcaaag gttgtattag tgactaacga
     3541 agtgggaatg gggattgtgc cggagagtcg tctggcacga cattttcgtg atattgccgg
     3601 gcgggtaaat cagcagttgg ccgctgcggc aaatgaagta tggctggtgg tttcgggtat
     3661 tggagtaaaa atcaaatgag taaattattt tgggcgatgc tctcatttat tacgcgtctg
     3721 cctgtaccgc gtcgctggtc ccagggactg gatttcgagc attattctcg cggtattatt
     3781 acttttcctt tgattggatt attgcttggc gcgattagcg ggctggtctt catggtgctg
     3841 caggcatggt gcggcgtacc actggcggca ctgtttagcg tacttgtgct ggcgctgatg
     3901 accgggggat tccacctgga cggcctcgct gatacctgtg atggcgtatt ttctgcacgt
     3961 agccgcgatc gcatgttgga aatcatgcgt gatagccgtt taggcaccca cggcggtctg
     4021 gcattgattt ttgtggtact ggcaaagatt ctggtgttaa gcgagttgac tctgcgtggc
     4081 gaaccgatcc ttgcatcgct ggcggcagca tgtgcggtca gccgcgggac tgccgcatta
     4141 ttgatgtatc gtcatcgtta tgcgcgggaa gaagggcttg gcaatgtgtt cattggcaaa
     4201 attgatgggc gacaaacctg cgtcacactc ggcttagccg ctatttttgc tgccgtatta
     4261 ttgccgggta tgcatggtgt ggctgctatg gtggtgacga tggtggtgat tttcatcctc
     4321 ggtcagcttc tcaaacgtac gctgggcggg caaacgggcg atacgctggg agcagccata
     4381 gagcttggtg aactggtttt cctgctggca ctgctataaa gagacgtgac atgcaaatac
     4441 ttgccgattt actgaatacg atcccagcca tcgatcctgc cgctatgtcg cgtgcacaac
     4501 ggcatattga cgggttactc aaacctgttg gtagcctggg aaagctggag gcgcttgcca
     4561 tacaactggc aggaatgccg gggttgaatg gcataccgca tgtgggcaaa aaagcgatac
     4621 tggttatgtg tgccgatcac ggcgtctggg aggaaggggt cgctatttct ccaaaagaag
     4681 tgacagccat acaggctgaa aatatgaccc gcggaacaac cggcgtgtgt gtgctggcag
     4741 cacaagcggg cgctaacgtc cacgtaattg atgttggtat tgatactgct gagcctatcc
     4801 ccgggcttat caacatgcgt gtcgcacgag gtagcggcaa tattgcttca gctccggcaa
     4861 tgagtcgccg tcaggctgaa aagttgcttt tggacgtcat atgttatacg cgggagctgg
     4921 caaaaaacgg tgtcacgctg tttggtgtag gtgaactggg gatggcaaac acgacaccgg
     4981 cagcggcaat agtcagcaca atcactggcc gggatcctga agaagtggtt gggattggcg
     5041 caaacctgcc gacagataaa ctggctaata aaattgatgt tgtgcgtcgg gcgattacgt
     5101 tgaatcaacc aaatcctcgg gatggtgttg atgtcctggc aaaagtgggt ggatttgatt
     5161 tggtcggaat agctggagtg atgttaggtg ctgcttcctg cggtttaccc gtgttgctgg
     5221 atggatttct ttcttatgct gctgcgctcg cagcctgcca gatgtctcct gcaatcaaac
     5281 cgtatctcat tccttctcac ttgtcggcag aaaaaggcgc gcgtatagcg ctgtcgcatt
     5341 tggggctgga gccttatctc aatatggata tgcgtttagg tgaggggagt ggtgcagctc
     5401 tggcgatgcc catcatcgaa gctgcttgtg cgatatacaa caatatgggc gaacttgctg
     5461 ccagtaatat tgttctaccg gggaatacga cttctgattt gaacagctaa aaacgaaaag
     5521 tctgataaaa gctatactta tcggatagta ttcgcgactt aggaaaggga catgatgcgt
     5581 cgtgtaaata ttctttgctc atttgctctg ctttttgcca gccaaaatag cctggcggta
     5641 acttatccat tacctccaga gggtagccgt ttagtggggc agtcgcttac tgtaactgtt
     5701 cctgatcaca atactcagcc gctggagact tttgccgcac aatacgggca agggttaagt
     5761 aatatgctgg aagcgaatcc gggcgctgat gtttttttgc cgaagtctgg ctcacaactc
     5821 accattccgc agcaactgat tttgcccgcc actgtccgta aagggattgt ggttaacgtc
     5881 gctgaaatgc gtctttatta ctatccgcca gacagtaata cagtggaagt ctttcctatt
     5941 ggtatcggcc aggctgggcg agaaaccccg cgtaactggg tgactaccgt tgaacgtaaa
     6001 caagaagcac caacctggac gccaacgccg aacactcggc gcgaatatgc gaaacgaggg
     6061 gagagtttgc ccgcatttgt tcctgcgggg cccgataacc ccatggggct gtacgcgatt
     6121 tatattggca ggttgtatgc catccacggt accaatgcca attttggtat tgggctccgg
     6181 gtaagtcagg gctgtattcg tctgcgcaat gacgatatca aatatctgtt tgataatgtt
     6241 cctgtaggca ctcgtgtaca agttattgat cagccagtga aatacacaat ggaaccggat
     6301 ggttcaaagt ggctggaagt tcatgagccg ctatcgcgca atcgtgcaga atatgagtct
     6361 gaccgaaaag tgccattgcc ggtaacccca tctttgcggg cgtttatcaa cgggcaagaa
     6421 gttgatgtaa atcgcgcaaa tgctgcgttg caacgtcgat cgggaaagcc tgtgaatatc
     6481 aaccgtaacg gattttgaca ttcaaaacgt ctggtttgtc ggtctgtgta agcattccgt
     6541 catccagaac aggttgtttc gaatcataca aatgataatt ttcttctcat atgcgcatct
     6601 cttatgagca taaacgttat actcttctga tttatacatg tttgcggatg acatgatcaa
     6661 tggaaaagag aatgacgaaa cagaataaaa acagccgttg gcagggttac gatcctcgtt
     6721 acatatactc gctcgtggtc agacgctatt ttgcggatat gaaaaccaag attgagattg
     6781 ctgaggagct tggcgtttcc cgctttaaag ttgccagatt gatcgatgag gcgatcgaac
     6841 aggattacgt gaggtttatc ttccctaagc agcaagcgat ggatgaagaa atcgccaata
     6901 atctacggaa aaaatttcat ctggaagatg caattgttct ttcagttgct gaatcatgga
     6961 cgacgcaaga agaactcaat cacaaattgg gtgaagtcac cgctgaatat cttatgcaat
     7021 ctcttcatga aggtatgaaa gtggggatcg cctggggacg tgtattatca agtacggtca
     7081 gtaagttgag caagctgcct cctttagacg ttgtgcagtt atctggcgta catccgggga
     7141 ttgagtttag tcaagggcca atagatctta ttcataagat cgctgccatt tcgcagggaa
     7201 aagcgcaccc aatgtacgtg ccgatgtggg tcgatgacga agagcttgct gccagactgg
     7261 caggtgatcc agcggtatta gatacacagc aatattactc gcagttggat gtggttatca
     7321 ccgggatagg tgactggaaa tcaggttctt caagcttgtg taaaatattt ccggatacct
     7381 ggtgcgaagc tttgtttcaa caagatatcg ctgcggatgt gtgtatctcg ttggtcagca
     7441 gggaagggaa gattcttcat agccctattg aacgtctggg atttggcatt tcgacggatc
     7501 aactacaaaa agccaaaaaa gtgattggtg tcgctggagg ggaggaaaaa tatgaaggca
     7561 ttcttgcttc gctgaaatct ggacttttaa acgtcttaat tactgatttt gatacggcta
     7621 ttaaacttct ggattaatag gcgtatcagt gaagaaaggg cagtaccgct gattctgccc
     7681 ttttaatttc aggatgctgc gagagtatcc tctacatatt tagctacgcg cgttgcaggt
     7741 aaattgcggc aagtctcaac aaaggcgtta tcctgaaaca gtgtgaataa acgacgcaat
     7801 atcgacatat gttgatcact ggtggcaaat gccaggttaa acataatgct acaatcaact
     7861 gttaaatcag catcttccat ttgcccgaaa gtgacatcat tggcgaaacg aacaatactg
     7921 atactgttta catttaccag cgtgtaatcg gcatggggaa tggcgatacc ctgaccgttt
     7981 gccatcatta gccctgtcgg atagcttttt tcacgttcca ggcaggcgtc aatgtattct
     8041 gcagtgatgt agcctttttc caataattgc gtaccagcaa aggtgatcgc agtttgatag
     8101 tcactgatgc tgttatcaac atcattcacc agaatttcaa atttcattgc acacctgtta
     8161 tttcaacatc tcttctttaa ctgcagcgaa aatctcatct tcgcccagac cagtcagtaa
     8221 tccagcgcca acgatgacac gagtagtaat ggccgggtta tcgagtttgg tcgatgaaac
     8281 taccaggtca tagctgttga aggtatcgct ggtgcatgcc attaacgaag atttatcgac
     8341 ttgcgcttca attttctctt ctttcaggaa gtcctgcagt acagagacca tgatggaaga
     8401 tgaggcaatt ccgtttccgc aaactactaa gatttttttc atttgattat tcctctgtaa
     8461 aagatttaaa gcgctttttc taaatccgtt tcaatctggc gacgtgattt ttgtttaatt
     8521 aagtatccaa tagataacaa aaggatacct gccatcacat agccaatgat tccaacatgg
     8581 ctcagggcgg caaacagacc agtaaatgga ttcgcagacg ggttaagcga agagtacatc
     8641 acgctggcat ccgggtttgt accaaacttc tggaagactt cgttggtagc cggtgccatc
     8701 cacgatgcaa tatacagacc accaccgaac aaaatgatcg aggtcaccca ggtgcgaata
     8761 atgttgccat tgaaaacagg caccatgcag gcaatgaaaa acacaaactg agagaggtca
     8821 cccagtggca taacgcggtt gccgggcaag atcactgcga ggatcacaat caccgggatc
     8881 agtaataatg ccggggcgat aactgacgga tgacccacaa tcagggcagt gtccattcca
     8941 acagtgattt gacgatccgg gaaacgtttg accagttttt tgcgtgccac gttggaaagc
     9001 ggaacaagcc cttccatcat gatagaggtc atacgtggca acagaagcat gactgcagcc
     9061 agatacatcc ctaactgtag gctggcaatt atggtgtggt agggggattt ccagccataa
     9121 ccacaaaagc ccaacaaaat accaataata aaaccaacca tagttggatc gcccagaatg
     9181 ccaaaccgtt tttgaatagt ttcggcggtc acattcagat tacggaagcc tgggatgcga
     9241 tcataaatcc agttgaatgg ataagcgaaa agtaagtagg tattggcaac cgggtgagag
     9301 atcgcaacac cttttggcag gttaaagaat ttttcgttat ttttagcacc gagatcggca
     9361 gcgatcagcg cccagacatg gaatagcgcg gcggtcaatg cgccatacag gaagctacca
     9421 gaatagacgc caacaattgc ccccattgac gcattttgat gaaggttgaa aacatcaacg
     9481 ttaacacagt cagtcaggcg ggcgaccagc attgcaatat ttaccgcaac acagagggga
     9541 ataatgatag cgcccatgct ggagccaaat cccatcactg cagtcgctgc gccaccaaca
     9601 tcgatggcac tgagcgagga acctgttgct tctgctaatc cggtgactgc cggttgcatt
     9661 ttttccagca tcagtttgat ggtcatggtt agcgcaatga aaccaatgcc gactttgatg
     9721 gcggaggaga tacattctga aagaggacgg ccaagtatct tcatcagtac cagaaagatg
     9781 accgggataa atataaaatt tcccagatcg tttataaatt gcgtgatcag ttccatacat
     9841 taaccctcag atttttcatc atctggtcgg aaacccatat tccagcgtac atcagcgggc
     9901 ttaaccagca atgcattatc gttacgttca gcttccagta agttcttttt agaattttct
     9961 ctggcggtgg tgaagtcgaa tgggttatcc aaccatgcat cgacaatcat tttgctgtat
    10021 tcgagatcgt tgacccggca acccagtcca ataacttgtg cgttattgct gagtcgcgcc
    10081 cgctttgcgg agtaggggtt agtaaccgca acggctctta tgccccagtg tttgttgatc
    10141 tgacaagtaa agccaatgcc agtcccacaa atgtaaattc ccagacggca ctcatcgcgt
    10201 tgaataatgc ctgccatttc gtcggccaga cgcgcgaagt tattgacacc ttgttcctta
    10261 catatgacgg gatcgtacac gatctccatg tttttctctt ctatgaggta tttttttatc
    10321 tcctctttac gtgcaaaacc tacatcatcg cttgcgatag caatcttcgt tttcatttca
    10381 ttttgcctgt c
//
LOCUS       DADSYM010000155        10245 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2415
            SAMN08039579-rid11723173.denovo.156, whole genome shotgun sequence.
ACCESSION   DADSYM010000155 DADSYM010000000
VERSION     DADSYM010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039579
            Sequence Read Archive: SRR6362533
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10245)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10245)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980162.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 03:39:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,484
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 4,347
            CDSs (with protein)               :: 4,347
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 43 of 85
            Pseudo Genes (incomplete)         :: 37 of 85
            Pseudo Genes (internal stop)      :: 19 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10245
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039579-rid11723173.denovo.156"
                     /strain="LGE2415"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8302 N 95.9628 W"
                     /collection_date="2015-08-24"
     gene            79..1473
                     /locus_tag="J1Y83_003691"
     CDS             79..1473
                     /locus_tag="J1Y83_003691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar porter family MFS transporter"
                     /protein_id="HBA7631802.1"
                     /translation="MPDAKKQGRSNKAMTFFVCFLAALAGLLFGLDIGVIAGALPFIA
                     DEFQITSHTQEWVVSSMMFGAAVGAVGSGWLSFKLGRKKSLMIGAILFVAGSLFSAAA
                     PNVEVLILSRVLLGLAVGVASYTAPLYLSEIAPEKIRGSMISMYQLMITIGILGAYLS
                     DTAFSYTGAWRWMLGVIIIPAILLLIGVFFLPDSPRWFAAKRRFVDAERVLLRLRDTS
                     AEAKRELDEIRESLQVKQSGWALFKENSNFRRAVFLGVLLQVMQQFTGMNVIMYYAPK
                     IFELAGYTNTTEQMWGTVIVGLTNVLATFIAIGLVDRWGRKPTLTLGFLVMAAGMGVL
                     GTMMHIGIHSPSAQYFAIAMLLMFIVGFAMSAGPLIWVLCSEIQPLKGRDFGITCSTA
                     TNWIANMIVGATFLTMLNTLGNANTFWVYAALNVLFILLTLWLVPETKHVSLEHIERN
                     LMKGRKLREIGAHD"
     gene            1550..2047
                     /locus_tag="J1Y83_003692"
     CDS             1550..2047
                     /locus_tag="J1Y83_003692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family zinc-dependent metalloprotease"
                     /protein_id="HBA7631803.1"
                     /translation="MKTSRLPIAIQQAVMRRLREKLAQANLKLGRNYPEPKLSYTQRG
                     TSAGTAWLESYEIRLNPVLLLENSEAFIEEVVPHELAHLLVWKHFGRVAPHGKEWKWM
                     MESVLGVPARRTHQFELQSVRRNTFPYRCKCQEHQLTVRRHNRVVRGEAVYRCVHCGE
                     QLVAK"
     gene            2142..2849
                     /gene="endA"
                     /locus_tag="J1Y83_003693"
     CDS             2142..2849
                     /gene="endA"
                     /locus_tag="J1Y83_003693"
                     /EC_number="3.1.21.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease I"
                     /protein_id="HBA7631804.1"
                     /translation="MYRYLSIAAVVLSAAFSGPALAEGINSFSQAKAAAVKVHADAPG
                     TFYCGCKINWQGKKGVVDLQSCGYQVRKNENRASRVEWEHVVPAWQFGHQRQCWQDGG
                     RKNCAKDPVYRKMESDMHNLQPSVGEVNGDRGNFMYSQWNGGEGQYGQCAMKVDFKEK
                     VAEPPARARGAIARTYFYMRDQYNLTLSRQQTQLFNAWDKMYPVTDWECERDERIAKV
                     QGNHNPYVQRACQARKS"
     gene            2929..3660
                     /gene="rsmE"
                     /locus_tag="J1Y83_003694"
     CDS             2929..3660
                     /gene="rsmE"
                     /locus_tag="J1Y83_003694"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311849.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HBA7631805.1"
                     /translation="MRIPRIYHPEPLTSHSHIALCEDAANHIGRVLRMGPGQALQLFD
                     GSNQVFDAEITSASKKSVEVKVLEGQIDDRESPLHIHLGQVMSRGEKMEFTIQKSIEL
                     GVSLITPLFSERCGVKLDSERLNKKLQQWQKIAIAACEQCGRNRVPEIRPAMDLEAWC
                     AEQDEGLKLNLHPRASNSINTLPLPVERVRLLIGPEGGLSADEIAMTARYQFTDILLG
                     PRVLRTETTALTAITALQVRFGDLG"
     gene            3673..4623
                     /gene="gshB"
                     /locus_tag="J1Y83_003695"
     CDS             3673..4623
                     /gene="gshB"
                     /locus_tag="J1Y83_003695"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione synthase"
                     /protein_id="HBA7631806.1"
                     /translation="MIKLGIVMDPIANINIKKDSSFAMLLEAQRRGYELHYMEMADLY
                     LINGEARARTRTLSVEQNYDKWYEFTGEQDLPLADLDVILMRKDPPFDTEFIYATYIL
                     ERAEDKGTLIVNKPQSLRDCNEKLFTAWFSDLTPETLVTRNKAQLKAFWEKHSDIILK
                     PLDGMGGASIFRVKEGDPNLGVIAETLTEHGTRYCMAQNYLPAIKDGDKRVLVVDGEP
                     VPYCLARIPQGGETRGNLAAGGRGEPRPLTESDWKIARQIGPTLKEKGLIFVGLDIIG
                     DRLTEINVTSPTCIREIEAEFPVSITGMLMDAIEARLQQQ"
     gene            4660..5295
                     /locus_tag="J1Y83_003696"
     CDS             4660..5295
                     /locus_tag="J1Y83_003696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708713.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HBA7631807.1"
                     /translation="MTAPFFPRILGAVAFLNQETEPLTMNLQHHFLIAMPALQDPIFR
                     RSVVYICEHNTNGAMGIIVNKPLENLKIEGILEKLKITPEPRDESIRLDKPVMLGGPL
                     AEDRGFILHTPPSNFASSIRISDNTVMTTSRDVLETLGTDKQPSDVLVALGYASWEKG
                     QLEQEILDNAWLTAPADLNILFKTPIADRWREAAKLIGVDILTMPGVAGHA"
     gene            5295..5711
                     /gene="ruvX"
                     /locus_tag="J1Y83_003697"
     CDS             5295..5711
                     /gene="ruvX"
                     /locus_tag="J1Y83_003697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HBA7631808.1"
                     /translation="MSGTLLAFDFGTKSIGVAVGQRITGTARPLPAIKAQDGTPDWNI
                     IERLLKEWQPDEIIVGLPLNMDGTEQPLTARARKFANRIHGRFGVEVKLHDERLSTVE
                     ARSGLFEQGGYRALNKGKVDSASAVIILESYFEQGY"
     gene            complement(5885..6865)
                     /locus_tag="J1Y83_003698"
     CDS             complement(5885..6865)
                     /locus_tag="J1Y83_003698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HBA7631809.1"
                     /translation="MNMEEIVALSVKHNVSDLHLCSAWPARWRIRGRMEAAPFDAPDV
                     EELLREWLDDDQRAILLENGQLDFAVSLAENQRLRGSAFAQRQGISLALRLLPSHCPQ
                     LEQLGAPPVLPELLKSENGLILVTGATGSGKSTTLAAMVGYLNQHADAHILTLEDPVE
                     YLYTSQRCLIQQREIGLHCMTFASGLRAALREDPDVILLGELRDSETIRLALTAAETG
                     HLVLATLHTRGAAQAVERLVDSFPAQEKDPVRNQLAGSLRAVLSQKLEVDKQEGRVAL
                     FELLINTPAVGNLIREGKTHQLPHVIQTGQQVGMITFQQSYQQRVKEGRL"
     gene            6883..7587
                     /gene="yggS"
                     /locus_tag="J1Y83_003699"
     CDS             6883..7587
                     /gene="yggS"
                     /locus_tag="J1Y83_003699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate homeostasis protein"
                     /protein_id="HBA7631810.1"
                     /translation="MNDIAHNLAQVRDKISAAATRCGRSPEEITLLAVSKTKPASAIA
                     EAIDAGQRQFGENYVQEGVDKIRHFQELGVTGLEWHFIGPLQSNKSRLVAEHFDWCHT
                     IDRLRIATRLNDQRPAELPPLNVLIQINISDENSKSGIQLAELDELAAAVAELPRLRL
                     RGLMAIPAPESEYVRQFEVARQMAVAFAGLKTRYPHIDTLSLGMSDDMEAAIAAGSTM
                     VRIGTAIFGARDYSKK"
     gene            7605..8171
                     /gene="yggT"
                     /locus_tag="J1Y83_003700"
     CDS             7605..8171
                     /gene="yggT"
                     /locus_tag="J1Y83_003700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotic shock tolerance protein YggT"
                     /protein_id="HBA7631811.1"
                     /translation="MNTLTFLLSTVIELYTMVLLLRIWMQWAHCDFYNPFSQFVVKVT
                     QPIIGPLRRVIPAMGPIDSASLLVAYILSFIKAIVLFKVVTFLPIIWIAGLLILLKTI
                     GLLIFWVLLVMAIMSWVSQGRSPIEYVLIQLADPLLRPIRRLLPAMGGIDFSPMILVL
                     LLYVINMGVAEVLQATGNMLLPGLWMAL"
     gene            8168..8458
                     /locus_tag="J1Y83_003701"
     CDS             8168..8458
                     /locus_tag="J1Y83_003701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggU family protein"
                     /protein_id="HBA7631812.1"
                     /translation="MSAVTVNDDGLVLRLYIQPKASRDSIVGLHGDEVKVAITAPPVD
                     GQANSHLVKFLGKQFRVAKSQVVIEKGELGRHKQIKIINPQQIPPEIAALIN"
     gene            8466..9059
                     /locus_tag="J1Y83_003702"
     CDS             8466..9059
                     /locus_tag="J1Y83_003702"
                     /EC_number="3.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XTP/dITP diphosphatase"
                     /protein_id="HBA7631813.1"
                     /translation="MQKVVLATGNAGKVRELASLLSDFGLDIVAQTDLGVDSAEETGL
                     TFIENAILKARHAAKVTGLPAIADDSGLAVDALGGAPGIYSARYSGEDATDQKNLQKL
                     LETMKDVPDDQRQARFHCVLVYLRHAEDPTPLVCHGSWPGVITREPAGTGGFGYDPIF
                     FVPSEGKTAAELTREEKSAISHRGQALKLLLDALRNG"
     gene            9052..10188
                     /gene="hemW"
                     /locus_tag="J1Y83_003703"
     CDS             9052..10188
                     /gene="hemW"
                     /locus_tag="J1Y83_003703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HBA7631814.1"
                     /translation="MVKLPPLSLYIHIPWCVQKCPYCDFNSHALKGEVPHDDYVQHLL
                     NDLNNDVAYAQGREVKTIFIGGGTPSLLSGPAMQTLLDGVRARLPLAADAEITMEANP
                     GTVEADRFVDYQRAGVNRISIGVQSFSEEKLKRLGRIHGPQEAKRAANLASGLGLRSF
                     NLDLMHGLPDQSLEEALGDLRQAIELNPPHLSWYQLTIEPNTLFGSRPPVLPDDDALW
                     DIFEQGHQLLTAAGYQQYETSAYSKPGYQCQHNLNYWRFGDYIGIGCGAHGKVTFPDG
                     RILRTTKTRHPRGFMQGRYLESQRDVEAADKPFEFFMNRFRLLEPAPRVEFSQYTGLS
                     EEVIRPQLDEAIAQGYLTECADYWQITEHGKLFLNSLLELFLAE"
BASE COUNT         2401 a         2691 c         2701 g         2452 t
ORIGIN      
        1 gtgatttgct tcacatcttt ttacgtcgta ctcacctatc ttaattcaca ataaaaaata
       61 accatattgg agggcatcat gcctgacgct aaaaaacagg ggcggtcaaa caaggcaatg
      121 acgtttttcg tctgcttcct tgccgctctg gcgggattac tctttggcct ggatatcggt
      181 gtaattgctg gcgcactgcc gtttattgca gatgaattcc agattacttc gcacacgcaa
      241 gaatgggtcg taagctccat gatgttcggt gcggcagtcg gtgcggtggg cagcggctgg
      301 ctctccttta aactcgggcg caaaaagagc ctgatgatcg gcgcaatcct gtttgttgcc
      361 ggttcactgt tctctgcggc tgcgccaaac gttgaagtac tgattctttc ccgcgttctg
      421 ctggggctgg cggtgggtgt ggcctcttat accgcaccgc tgtacctctc tgaaattgcg
      481 ccggaaaaaa ttcgcggcag tatgatctcg atgtatcagt tgatgatcac tatcgggatc
      541 ctcggcgctt atctttctga taccgccttc agctacaccg gtgcatggcg ctggatgctg
      601 ggtgtgatta tcatcccggc aattttgctg ctgattggtg tcttcttcct gccagacagc
      661 ccacgttggt ttgccgccaa acgccgtttt gttgatgccg aacgcgtgct gctacgcctg
      721 cgtgacacca gcgcggaagc gaaacgcgaa ctggatgaaa tccgtgaaag tttgcaggtt
      781 aaacagagtg gctgggcgct gtttaaagag aatagcaact tccgccgcgc ggtgttcctt
      841 ggcgtactgt tgcaggtaat gcagcaattc accgggatga acgtcatcat gtattacgcg
      901 ccgaaaatct tcgaactggc gggttatacc aacaccaccg agcaaatgtg ggggacagtg
      961 attgtcggcc tgaccaacgt acttgccacc tttatcgcaa tcggccttgt tgaccgctgg
     1021 ggacgtaaac caacgctaac gctgggcttc ctggtgatgg ctgctggtat gggcgtactc
     1081 ggtacaatga tgcatatcgg tatccactct ccgtcggcgc agtatttcgc catcgccatg
     1141 ctgctgatgt ttattgtcgg ttttgccatg agtgccggtc cgctgatttg ggtactgtgc
     1201 tccgaaattc agccgctgaa aggccgcgat tttggcatca cctgctccac tgccaccaac
     1261 tggattgcca acatgatcgt tggcgcaacg ttcctgacca tgctcaacac gctgggtaac
     1321 gccaacacct tctgggtgta tgctgctctg aacgtactgt ttatcctgct gacattgtgg
     1381 ctggtaccgg aaaccaaaca cgtttcgctg gaacatattg aacgtaatct gatgaaaggt
     1441 cgtaaactgc gcgaaatcgg cgctcacgat taatctcctc aggcttcctc ccatcatgga
     1501 ggaagccacc tcttgcagtc atcttttctt cgctctatcc tctgccgcta tgaaaacatc
     1561 ccgtctccct atcgccatcc aacaggccgt tatgcgtcgc ctgcgggaaa aactcgccca
     1621 ggccaacctg aagctagggc gtaactaccc ggagccaaaa ctctcttaca cccagcgcgg
     1681 aacctctgcc ggaacagcct ggctggaaag ctatgaaatt cgcctcaatc ccgttttgct
     1741 gttggaaaac agtgaagcct ttattgaaga agtggtgccg cacgaactgg cacatttgct
     1801 ggtatggaaa catttcggcc gcgtagcgcc acatggcaaa gagtggaagt ggatgatgga
     1861 aagcgtgctg ggcgttcccg cccgccgtac gcatcagttc gaactgcaat ccgtgcgccg
     1921 caacaccttc ccctaccgct gcaagtgcca ggagcatcag cttaccgtac gccgtcataa
     1981 tcgcgtagtt cgtggcgaag ccgtctatcg ctgtgttcac tgcggcgaac agctggttgc
     2041 gaaataacca cctgaactat caggaacttt cctgatctgg ctgattgcat accaaaacaa
     2101 ctttcgctac gttgctggct cgttttaaca cggagtaagt gatgtaccgt tatttgtcta
     2161 ttgctgcggt ggtactgagc gcagcatttt ccggcccggc gttggccgaa ggtatcaata
     2221 gtttttctca agcgaaagcc gcggcggtaa aagtccacgc tgacgcgccc ggtacgtttt
     2281 attgcggatg taaaattaac tggcagggca aaaaaggcgt tgttgatctg caatcgtgcg
     2341 gctatcaggt gcgaaaaaat gaaaaccgtg ccagccgcgt agagtgggaa cacgtcgttc
     2401 ctgcctggca attcggtcac cagcgccagt gctggcagga cggtggacgt aaaaattgcg
     2461 ccaaagatcc ggtctatcgc aagatggaaa gcgatatgca taacctgcag ccgtcagtcg
     2521 gtgaggtgaa tggcgatcgc ggcaacttta tgtacagcca gtggaatggc ggtgaaggcc
     2581 agtacggtca atgcgccatg aaggtcgatt tcaaagaaaa agttgccgaa ccaccagcgc
     2641 gtgcacgcgg tgccattgcg cgcacctact tctatatgcg tgaccaatac aacctgacac
     2701 tctctcgtca gcaaacgcag ctgttcaacg catgggacaa gatgtatcca gttaccgact
     2761 gggagtgcga gcgtgatgaa cgcatcgcga aggtgcaggg caatcataac ccgtatgtgc
     2821 aacgcgcttg ccaggcgcga aagagctaac ctacactagc gggattcttt ttgttaaccc
     2881 ctaccccacg cgtacaaccg cgtggggaga cgacgcggat ttttaactat gcgtatcccc
     2941 cgcatttatc atcctgaacc actgaccagc cattctcaca tcgcgctttg cgaagatgcc
     3001 gccaaccata tcgggcgcgt actgcgtatg gggccgggac aggcgctgca attgtttgac
     3061 ggcagcaacc aggtctttga cgccgaaatt accagcgcca gcaaaaaaag cgtggaagtg
     3121 aaggtgctgg aaggccagat cgacgatcgc gaatctccgc tgcatattca cctcggtcag
     3181 gtgatgtcgc gtggtgaaaa aatggaattt actatccaga aatcgatcga actcggtgta
     3241 agcctcatta cgccactttt ttctgagcgc tgcggcgtta aactggatag tgaacgtctg
     3301 aacaagaagc tccagcagtg gcagaagatt gcaattgctg cctgtgagca gtgtggccgt
     3361 aaccgggtgc cggaaatccg tccagcgatg gatctggaag cctggtgtgc agagcaggat
     3421 gaaggactga aactgaatct tcacccgcgc gccagtaaca gcatcaatac gttgccgtta
     3481 ccggtcgaac gcgtccgcct gctgattggc ccggaaggcg gtttatcggc agatgaaatt
     3541 gccatgactg cccgctatca atttactgat atcctgttgg gacctcgcgt tttgcgtact
     3601 gagacaactg cgctcaccgc cattaccgcg ctacaagtgc gttttggcga tttgggctaa
     3661 cggagaagaa taatgatcaa gctcggcata gtgatggacc ccatcgcaaa catcaacatc
     3721 aagaaagatt ccagtttcgc tatgttgctg gaagcacagc gtcgtggtta cgaacttcac
     3781 tatatggaga tggccgatct ctatttgatc aatggtgagg ctcgcgctcg tacccgtact
     3841 ttgagcgtcg aacagaacta cgataaatgg tacgagttca cgggtgagca ggatctgccg
     3901 ctggccgatc tcgatgtgat cctgatgcgt aaagacccgc cgtttgatac cgagtttatc
     3961 tacgcgacct atattctgga acgtgccgaa gataaaggga cgctgatcgt taacaagccg
     4021 cagagcctgc gcgattgtaa cgaaaaactg ttcactgcct ggttctctga cttaacgcca
     4081 gaaacgctgg tcacacgcaa taaagcgcag ctaaaagctt tctgggaaaa acacagcgac
     4141 atcattctta agccgctgga cggtatgggc ggcgcgtcga ttttccgcgt gaaagaaggt
     4201 gatccaaacc tcggcgtgat tgccgaaacc ctgactgagc atggcactcg ctactgcatg
     4261 gcgcaaaatt acctgccagc cattaaagat ggcgacaaac gcgtgctggt agtggatggc
     4321 gagccggtgc cgtattgcct ggcgcgtatt ccgcaggggg gcgaaacccg tggcaatctg
     4381 gctgccggtg gtcgcggtga acctcgtccg ctgacggaaa gtgactggaa aatcgcccgt
     4441 cagattgggc cgacgctgaa agaaaaaggg ctgatttttg ttggtctgga tatcatcggc
     4501 gaccgtctga ctgaaattaa cgtcaccagc ccaacctgta ttcgtgagat tgaagcagag
     4561 tttccggtgt cgattaccgg aatgttaatg gatgccatcg aagcacgttt acagcagcag
     4621 taacccacct tagcgagaag gatctcgttg agactctgag tgacagcgcc cttctttcca
     4681 cgcatactgg gcgctgttgc ttttttgaac caggaaacag aacctctgac aatgaattta
     4741 cagcatcact ttcttattgc catgcctgct ctccaggatc cgattttccg tcgttccgtg
     4801 gtctacattt gcgaacataa taccaatggt gcaatgggga tcatcgtcaa caagccgctg
     4861 gaaaatctca aaattgaagg gattctggaa aaactgaaga tcacgccgga gccgcgtgat
     4921 gaatcaatcc gtctggataa accggttatg ctcggcggtc cgctggctga agatcgcggg
     4981 tttattttgc atactccgcc ctccaatttt gcttccagca ttcgcatttc agacaacaca
     5041 gtgatgacca cttcccgcga tgtgctggaa acgctcggca ccgataaaca accgtctgac
     5101 gtattggtgg ctctgggtta tgcctcctgg gagaaaggtc aactggaaca agaaattctc
     5161 gataacgcgt ggctaacggc cccggcagat ctgaatattc tgttcaaaac gccgattgcg
     5221 gaccgctggc gcgaggcggc aaaactgatt ggtgtggata ttctcaccat gcctggtgtg
     5281 gcaggacacg cctgatgagt ggaaccttac tcgccttcga cttcggcacc aaaagcattg
     5341 gcgtagcggt cggccaacgc attaccggca ccgctcgccc tttgcctgca attaaagcgc
     5401 aggacggtac gccggactgg aacattatcg agcgtttact gaaagagtgg cagccggacg
     5461 aaatcatcgt cggtttgccg ctgaatatgg acggcaccga acagccgtta accgccagag
     5521 cgcgtaaatt tgccaaccgt attcatggtc gtttcggtgt tgaagtaaag ctccatgacg
     5581 agcgtcttag caccgtggag gcacgttccg gtctgtttga acagggcggc tatcgggcgc
     5641 tcaacaaagg caaagtcgac tctgcctctg cggttattat tctcgaaagc tatttcgagc
     5701 agggatatta aggcgattta ctagcgcaca gatttctgac aatgtgctcg ttgtttcatg
     5761 ttggatgcgg cgtaaacgcc ttatccaacc tacagtgagt gcgaattcaa agttacgctt
     5821 cccttgtagg cccgataagc ttgcgcatcg ggcatattta caattcgtat cagacacagc
     5881 aacgtcacaa gcgcccttct ttcacccgct gctgataact ctgctgaaac gttatcatcc
     5941 ccacctgctg cccggtttga ataacatgcg gtaactggtg ggttttccct tcacgaatca
     6001 aattccccac cgcgggtgtg ttaatcagta attcaaacag cgccacgcgt ccttcctgtt
     6061 tatccacttc cagtttttgt gacaacactg cccgtaaact acctgccagt tgattacgca
     6121 cgggatcttt ttcctgcgca ggaaatgaat ccaccagtcg ctcaactgcc tgcgccgcac
     6181 cacgcgtatg taatgttgca agcaccaaat gcccggtttc tgccgccgtc agcgccagac
     6241 ggattgtctc gctgtcacgc agctctccga gcaaaatcac atcaggatct tcccgcaatg
     6301 cagcccgcaa tcccgatgcg aaagtcatac agtgcaaacc aatctcccgc tgctggatca
     6361 aacatcgctg actggtatag agatattcca caggatcttc cagcgtcaga atatgcgcat
     6421 cggcatgttg attgagatag ccaaccatcg ccgccagcgt ggtagatttg ccgctccccg
     6481 tcgcccccgt caccagaatc aggccattct cgctcttgag taattccggc aataccggtg
     6541 gtgcgccaag ctgttcgagc tgcgggcagt gcgaaggtaa cagccgtaac gccagagaaa
     6601 taccttgccg ttgtgcgaac gcactgccgc gcaatcgctg gttttccgcc agcgacacag
     6661 caaaatccag ctgaccattc tccagcaata ttgcccgctg atcgtcatcc agccactccc
     6721 gcagtagctc ttcgacgtcc ggcgcgtcaa acggcgcagc ttccattctc ccgcgaatac
     6781 gccatcgtgc gggccaggcg ctgcacaggt gtagatccga gacgttatgc tttacactaa
     6841 gggccacaat ttcttccata ttcatactaa gatcctcgga aaatgaacga tattgcgcat
     6901 aacctggcac aggtccggga caaaatctca gcggctgcaa cgcgttgcgg ccgttctcca
     6961 gaagaaatta cgctgcttgc agtcagtaaa acaaaacctg cgagcgccat cgcagaagcc
     7021 attgatgccg ggcagcgtca atttggtgaa aactacgttc aggaaggggt agataaaatt
     7081 cgccactttc aggaactggg cgtaacagga ttagaatggc attttattgg cccgttgcag
     7141 tctaataaaa gccgcctggt ggcagagcat ttcgactggt gtcataccat cgaccgtttg
     7201 cgcatcgcta cccgcctgaa cgaccagcgc ccggcagaac ttccccctct taacgtactg
     7261 attcaaatta acattagtga tgaaaacagt aagtccggga ttcaactggc tgaactggac
     7321 gagctggcag ctgcggtcgc tgaactaccg cgtttacgtc tgcgcgggct gatggcaatc
     7381 cctgcgcctg agtcagaata tgtaaggcag tttgaagttg cacgccaaat ggctgtagca
     7441 tttgccggac tgaaaacgcg ctacccgcat atcgacacgc tctctctggg aatgtcggac
     7501 gatatggaag ccgccattgc ggcaggtagc acgatggttc gtatcggcac tgcaattttt
     7561 ggtgcgcgtg attactctaa aaaataagga attaaaggaa cgccatgaat acgttgactt
     7621 tcctgctttc aacggtcatt gagctgtata ccatggtgct gttattacgc atctggatgc
     7681 agtgggctca ttgtgatttt tacaacccct tctcacagtt tgtagtgaag gtaacgcagc
     7741 cgattatcgg gccactgcgc cgcgttattc cggcaatggg ccccattgat agcgcctcgc
     7801 tgctggttgc ctatattctc agtttcatta aagccatcgt gctgtttaaa gtggtgacct
     7861 tcctgccaat catttggatt gccggtttat tgattctgct gaaaaccatc ggcctgctga
     7921 ttttctgggt cctgctggtg atggcgatta tgagctgggt aagccagggt cgtagcccga
     7981 ttgaatacgt gctgattcag ctggccgatc cgctgctgcg cccgattcgc cgcttgctac
     8041 cggcaatggg tgggattgat ttctcgccga tgatcctcgt tctgctgctg tatgtcatca
     8101 atatgggtgt cgcagaagtc ttgcaggcga ccggaaatat gctgctgccg gggctgtgga
     8161 tggcgttatg agtgccgtaa cagttaatga tgacggtctg gttttacggc tctatattca
     8221 gccgaaagcc agccgtgatt ctattgtcgg tttacatggc gacgaggtaa aagtcgccat
     8281 taccgcgccg ccggttgacg gccaggccaa cagccatctg gtgaagtttc tcggtaagca
     8341 attccgggtt gccaaaagcc aggtggtaat tgaaaaaggc gaacttggcc gccacaaaca
     8401 aattaaaatc attaatccgc aacaaatccc gccagaaatc gcggcgttaa ttaattaggt
     8461 atcctatgca aaaagttgtc ctcgcaaccg gcaatgccgg taaagtgcgt gagctggcgt
     8521 cgctgcttag cgacttcggt cttgatatcg tggcccaaac agacctcggc gtagattccg
     8581 ctgaagaaac cggcctgacc tttatcgaaa acgcgattct gaaagcgcgc catgcggcaa
     8641 aagtgaccgg tttaccggca attgccgacg actccggtct ggcggtagat gcgctgggtg
     8701 gcgcgccggg tatttactcc gcgcgttatt ccggtgaaga cgcgaccgat caaaagaatc
     8761 tgcaaaaact gctggaaaca atgaaagacg taccggacga ccaacgtcag gcgcgtttcc
     8821 actgcgtgct ggtatatctg cgtcacgcgg aagatcccac tccgctggtg tgccacggta
     8881 gctggccggg cgtgattact cgtgaaccag cgggcactgg tggctttggt tatgatccaa
     8941 tcttcttcgt accttccgaa gggaaaactg ctgccgaact gacccgcgaa gaaaagagcg
     9001 ccatttccca ccgtggtcag gcgttgaaac tgctgctgga cgctttacgt aatggttaaa
     9061 ttaccgccgc tgagtctcta cattcacatc ccgtggtgcg tgcagaaatg cccgtactgc
     9121 gatttcaact ctcacgcgtt gaaaggagaa gtgccgcacg acgattatgt tcagcatctg
     9181 cttaacgatc tgaacaacga tgtggcttac gctcagggcc gtgaagtaaa gacaatcttt
     9241 attggcggtg gtacgccgag cctgctttcc ggcccggcga tgcaaacgct gctggacggc
     9301 gtacgtgcgc gtttgccgct ggcagcggat gcagaaatta cgatggaagc gaatcctggt
     9361 acggttgaag ccgatcgctt tgttgattat cagcgtgctg gtgtgaaccg catctccatt
     9421 ggcgtacaga gttttagcga agaaaagctg aaacgacttg ggcgcattca tggcccgcaa
     9481 gaagcgaaac gagcggcaaa tctggcgagc ggtttagggt tacgtagctt taacctcgat
     9541 ttgatgcatg ggctgccgga tcaatcactg gaagaggcgc taggcgatct gcgccaggct
     9601 attgaactga atccgccgca tctttcctgg tatcaactga ccatcgaacc taatacgctg
     9661 tttggctcgc gcccaccggt gctgccggac gacgatgcgt tgtgggatat attcgaacag
     9721 gggcatcagt tattaaccgc agcgggttat cagcaatatg aaacttccgc ttactccaaa
     9781 ccaggttatc agtgccagca caatctcaac tactggcgct ttggtgacta catcggtatt
     9841 ggctgcggcg cgcatggcaa agtgaccttc ccggatgggc gcattctgcg taccaccaaa
     9901 acgcgtcatc cgcgtggttt tatgcagggg cggtatctgg aaagccagcg agatgtcgaa
     9961 gccgcagata aaccatttga gttctttatg aatcgcttcc gtttgctgga acctgcgccg
    10021 cgcgtggagt ttagccagta tactggcctt tcagaagagg ttattcgccc tcagttagac
    10081 gaggctattg ctcagggtta tctcacagaa tgtgcggatt actggcagat aaccgagcac
    10141 ggtaaattat tcttaaactc acttcttgaa ttattcctcg cagagtaatc attaatgcgc
    10201 ctgcgggcgc attaatcact tcttttataa atcgcccgct aatta
//
LOCUS       DADSYM010000156        10193 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2415
            SAMN08039579-rid11723173.denovo.157, whole genome shotgun sequence.
ACCESSION   DADSYM010000156 DADSYM010000000
VERSION     DADSYM010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039579
            Sequence Read Archive: SRR6362533
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10193)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10193)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980162.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 03:39:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,484
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 4,347
            CDSs (with protein)               :: 4,347
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 43 of 85
            Pseudo Genes (incomplete)         :: 37 of 85
            Pseudo Genes (internal stop)      :: 19 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10193
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039579-rid11723173.denovo.157"
                     /strain="LGE2415"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8302 N 95.9628 W"
                     /collection_date="2015-08-24"
     gene            complement(211..531)
                     /locus_tag="J1Y83_003704"
     CDS             complement(211..531)
                     /locus_tag="J1Y83_003704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000590027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA7631815.1"
                     /translation="MIKIDEIDFFAFEGCSESEVTELLGKKPTGYNDWIINIKTGVEW
                     VFYDGRYWLDFRKSKEGIRCISCSILYHDKSKGRIDVLFSEGIADRGLKNTGGVLIQK
                     RPQL"
     gene            complement(591..4274)
                     /gene="metH"
                     /locus_tag="J1Y83_003705"
     CDS             complement(591..4274)
                     /gene="metH"
                     /locus_tag="J1Y83_003705"
                     /EC_number="2.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000096005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HBA7631816.1"
                     /translation="MSSKVEQLRAQLNERILVLDGGMGTMIQSYRLNEADFRGERFAD
                     WPCDLKGNNDLLVLSKPEVIAAIHNAYFEAGADIIETNTFNSTTIAMADYQMESLSAE
                     INFAAAKLARACADEWTARTPEKPRYVAGVLGPTNRTASISPDVNDPAFRNITFDQLV
                     AAYRESTKALVEGGADLILIETVFDTLNAKAAVFAVKTEFEALGVELPIMISGTITDA
                     SGRTLSGQTTEAFYNSLRHAEALTFGLNCALGPDELRQYVQELSRIAECYVTAHPNAG
                     LPNAFGEYDLDADTMAKQIREWAEAGFLNIVGGCCGTTPQHIAAMSRAVEGLAPRKLP
                     EIPVACRLSGLEPLNIGEDSLFVNVGERTNVTGSAKFKRLIKEEKYSEALDVARQQVE
                     NGAQIIDINMDEGMLDAEAAMVRFLNLIAGEPDIARVPIMIDSSKWDVIEKGLKCIQG
                     KGIVNSISMKEGVDAFIHHAKLLRRYGAAVVVMAFDEQGQADTRARKIEICRRAYKIL
                     TEEVGFPAEDIIFDPNIFAVATGIEEHNNYAQDFIGACEDIKRELPHALISGGVSNVS
                     FSFRGNDPVREAIHAVFLYYAIRNGMDMGIVNAGQLAIYDDLPTELRDAVEDVILNRR
                     DDGTERLLELAEKYRGSKTDDTANTQQAEWRSWEVNKRLEYSLVKGITEFIEQDTEEA
                     RQQATRPIEVIEGPLMDGMNVVGDLFGEGKMFLPQVVKSARVMKQAVAYLEPFIEASK
                     EQGKTNGKMVIATVKGDVHDIGKNIVGVVLQCNNYEIVDLGVMVPAEKILRTAKEVNA
                     DLIGLSGLITPSLDEMVNVAKEMERQGFTIPLLIGGATTSKAHTAVKIEQNYSGPTVY
                     VQNASRTVGVVAALLSDTQRDDFVARTRKEYETVRIQHGRKKPRTPPVTLEAARDNDF
                     AFDWQAYTPPVAHRLGVQEVEASIETLRNYIDWTPFFMTWSLAGKYPRILEDEVVGVE
                     AQRLFKDANDMLDKLSAEKMLNPRGVVGLFPANRVGDDIEIYRDETRTHVINVSHHLR
                     QQTEKTGFANYCLADFVAPKVSGKADYIGAFAVTGGLEEDALADAFEAQHDDYNKIMV
                     KALADRLAEAFAEYLHERVRKVYWGYAPNENLSNEELIRENYQGIRPAPGYPACPEHT
                     EKATIWELLEVEKHTGMKLTESFAMWPGASVSGWYFSHPDSKYYAVAQIQRDQVEDYA
                     RRKGMSVTEVERWLAPNLGYDAE"
     gene            4474..5298
                     /gene="iclR"
                     /locus_tag="J1Y83_003706"
     CDS             4474..5298
                     /gene="iclR"
                     /locus_tag="J1Y83_003706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate bypass operon transcriptional
                     repressor IclR"
                     /protein_id="HBA7631817.1"
                     /translation="MVAPIPAKRGRKPAVATAPATGQVQSLTRGLKLLEWIAESNGSV
                     ALTELAQQAGLPNSTTHRLLTTMQQQGFVRQVGELGHWAIGAHAFMVGSSFLQSRNLL
                     AIVHPILRNLMEESGETVNMAVLDQSNHEAIIIDQVQCTHLMRMSAPIGGKLPMHASG
                     AGKAFLAQLSEEQVTKLLHRKGLHAYTHATLVSPVHLKEDLAQTRKRGYSFDDEEHAL
                     GLRCLAACIFDEHREPFAAISISGPISRITDDRVTEFGAMVIKAAKEVTLAYGGMR"
     gene            complement(5315..7039)
                     /gene="aceK"
                     /locus_tag="J1Y83_003707"
     CDS             complement(5315..7039)
                     /gene="aceK"
                     /locus_tag="J1Y83_003707"
                     /EC_number="2.7.11.5"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001137229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional isocitrate dehydrogenase
                     kinase/phosphatase"
                     /protein_id="HBA7631818.1"
                     /translation="MPRGLELLIAQTILQGFDAQYGRFLEVTSGAQQRFEQADWHAVQ
                     QAMKNRIHLYDHHVGLVVEQLRCITNGQSTDAAFLLRVKEHYTRLLPDYPRFEIAESF
                     FNSVYCRLFDHRSLTPERLFIFSSQPERRFRTIPRPLAKDFHPDHGWESLLMRVISDL
                     PLRLRWQNKSRDIHYIVRHLTETLGTDNLAESHLQVANELFYRNKAAWLVGKLITPSG
                     TLPFLLPIHQTDDGELFIDTCLTTTAEASIVFGFARSYFMVYAPLPAALVEWLREILP
                     GKTTAELYMAIGCQKHAKTESYREYLVYLQGCNEQFIEAPGIRGMVMLVFTLPGFDRV
                     FKVIKDRLAPQKEMSAAHVRACYQLVKEHDRVGRMADTQEFENFVLEKRHISPALMEL
                     LLQEAAEKITDLGEQIVIRHLYIERRMVPLNIWLEQVEGQQLRDAIEEYGNAIRQLAA
                     ANIFPGDMLFKNFGVTRHGRVVFYDYDEICYMTEVNFRDIPPPRYPEDELASEPWYSV
                     SPGDVFPEEFRHWLCADPRIGPLFEEMHADLFRADYWRALQNRIREGHVEDVYAYRRR
                     QRFSVRFV"
     gene            complement(7125..8429)
                     /gene="aceA"
                     /locus_tag="J1Y83_003708"
     CDS             complement(7125..8429)
                     /gene="aceA"
                     /locus_tag="J1Y83_003708"
                     /EC_number="4.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase"
                     /protein_id="HBA7631819.1"
                     /translation="MKTRTQQIEELQKEWTQPRWEGITRPYSAEDVVKLRGSVNPECT
                     LAQLGAAKMWRLLHGESKKGYINSLGALTGGQALQQAKAGIEAVYLSGWQVAADANLA
                     ASMYPDQSLYPANSVPAVVERINNTFRRADQIQWSAGIEPGDPRYVDYFLPIVADAEA
                     GFGGVLNAFELMKAMIEAGAAAVHFEDQLASVKKCGHMGGKVLVPTQEAIQKLVAARL
                     AADVTGVPTLLVARTDADAADLITSDCDPYDSEFITGERTSEGFFRTHAGIEQAISRG
                     LAYAPYADLVWCETSTPDLELARRFAEAIHAKYPGKLLAYNCSPSFNWQKNLDDKTIA
                     SFQQQLSDMGYKFQFITLAGIHSMWFNMFDLANAYAQGEGMKHYVEKVQQPEFAAAKD
                     GYTFVSHQQEVGTGYFDKVTTIIQGGTSSVTALTGSTEESQF"
     gene            complement(8459..10060)
                     /gene="aceB"
                     /locus_tag="J1Y83_003709"
     CDS             complement(8459..10060)
                     /gene="aceB"
                     /locus_tag="J1Y83_003709"
                     /EC_number="2.3.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase A"
                     /protein_id="HBA7631820.1"
                     /translation="MTEQATTIDELAFTRPYGEQEKQILTAEAVEFLTELVTHFTPQR
                     NKLLAARIQQQQDIDNGMLPDFISETASIRDADWKIRGIPADLEDRRVEITGPVERKM
                     VINALNANVKVFMADFEDSLAPDWNKVIDGQINLRDAVNGTISYSNEAGKIYQLKPNP
                     AVLICRVRGLHLPEKHVTWRGETIPGSLFDFALYFFHNYQALLAKGSGPYFYLPKTQS
                     WQEAAWWSEVFSYAEDRFNLPRGTIKATLLIETLPAVFQMDEILHALRDHIVGLNCGR
                     WDYIFSYIKTLKNYPDRVLPDRQAVTMDKPFLNAYSRLLIKTCHKRGAFAMGGMAAFI
                     PSKDEERNNQVLDKVKADKSLEANNGHDGTWIAHPGLADTAMAVFNDILGSRKNQLEV
                     MREQDAPITADQLLAPCDGERTEEGMRANIRVAVQYIEAWISGNGCVPIYGLMEDAAT
                     AEISRTSIWQWIHHQKTLSNGKPVTKALFRQMLGEEMKVIASELGEERFSHGRFDDAA
                     RLMEQITTSDELIDFLTLPGYHLLA"
BASE COUNT         2355 a         2818 c         2549 g         2471 t
ORIGIN      
        1 aaaaccgcct tcaacaccga tatccttaag taaagaaagt gaatgaacga tatcggaata
       61 aaaagggata cgatcattac caagcgagta gaacagatat gtggctatcg cgataacatc
      121 cataacaccc caaaaaagaa gaatgtattt tttatattga cgttgcataa agtaaatcct
      181 ttttacgaaa acaaatatta ccaggaatta ttataattgc gggcgttttt ggattaacac
      241 tccacccgta ttttttaaac ctctgtcagc tataccttca ctaaacaaga catcgatacg
      301 ccctttactt ttatcgtgat acagaatact gcatgagata caccttatac cctctttgga
      361 tttacgaaaa tctaaccaat aacgaccatc ataaaatacc cactccacac ctgtttttat
      421 attaattatc caatcattat aacctgttgg ttttttccct aatagttcag taacttcgga
      481 ctctgaacat ccttcgaaag caaaaaaatc tatttcatca atttttatca tgttcaataa
      541 tccattattt atgacattaa aaaccggggc aaataccccg gtgttatcac ttactcggca
      601 tcatacccca gattcggtgc caaccaacgc tcgacttcgg taacgctcat tcctttacgg
      661 cgggcatagt cttcaacctg atcgcgctga atttgcgcca ccgcgtaata cttgctgtct
      721 ggatgactga agtaccaacc cgaaaccgat gcacccggcc acatggcgaa ggattccgtg
      781 agtttcatac cggtatgttt ttccacttcc agcagctccc agattgtggc tttttccgta
      841 tgttccgggc aggcagggta gcccggtgcc ggacggatgc cctggtagtt ttcgcggatc
      901 agctcttcgt tgctgaggtt ctcgttcggc gcatagcccc agtagacttt acgcacacgc
      961 tcatggagat actccgcaaa ggcttccgcc aggcggtcgg caagcgcttt caccatgatt
     1021 ttgttgtaat catcatgctg tgcttcaaag gcatcagcca gtgcgtcctc ttccagcccg
     1081 ccagtcacgg caaatgcgcc gatgtaatct gctttgccgg aaaccttcgg cgcaacgaag
     1141 tcagcgagac agtagttagc gaagccggtt ttttcggtct gttggcgtag atggtggctg
     1201 acattaatca catgggtacg cgtttcgtca cggtagattt caatgtcatc gcccacacgg
     1261 tttgccggga acaggcccac cacaccacgc gggttcagca ttttttcggc gcttaattta
     1321 tccagcatgt cgttggcgtc tttaaacagc cgctgcgcct caacacctac cacttcatct
     1381 tccagaatgc gcggatactt cccggccagc gaccaggtca taaagaacgg cgtccagtcg
     1441 atgtaattac gcagcgtttc gatgctggct tcgacttcct gcacaccgag acggtgcgcc
     1501 accggcggcg tgtaagcctg ccagtcaaaa gcgaagtcgt tatcacgcgc cgcctccagc
     1561 gtgaccggtg gtgtgcgtgg tttctttcgt ccgtgctgaa tacgcacggt ttcgtactcc
     1621 ttgcgggtac gagcaacgaa atcatcgcgc tgagtgtcgg aaagcaacgc cgccaccaca
     1681 ccaacggtgc gtgatgcgtt ctgcacatac accgtcgggc cgctgtagtt ctgctcgatt
     1741 ttcaccgccg tgtgcgcttt tgaggtggtc gcgccgccaa tcaatagcgg aatggtgaag
     1801 ccctgacgct ccatctcttt cgccacgtta accatctcgt ccagcgacgg cgtgatcagc
     1861 cccgaaaggc caatcaaatc agcattcact tctttagcgg tacggagaat tttttccgca
     1921 ggcaccatca cgccgagatc gacaatttcg tagttgttac attgcagcac cacgccaacg
     1981 atatttttac cgatgtcgtg gacatcgcct ttcacggtgg cgatcaccat cttgccgttg
     2041 gttttaccct gctctttgct ggcttcaata aacggttcga gatacgccac cgcctgcttc
     2101 attacgcgcg ccgatttcac cacctgcggc aggaacattt taccctcgcc aaacaggtcg
     2161 ccgaccacgt tcatgccgtc catcaacggg ccttcaatca cttcaatcgg acgcgtagcc
     2221 tgctggcggg cttcttcggt atcctgctcg ataaactcgg taatgccttt gaccagcgag
     2281 tattccagac gtttattcac ttcccacgag cgccactccg cctgctgggt gttagcggtg
     2341 tcgtcggttt tgctgccgcg atatttctcg gcaagctcca gtaaacgctc ggtgccatcg
     2401 tcgcgacggt tgaggatcac atcctccacc gcgtcgcgta gttcggtagg caggtcgtcg
     2461 taaatcgcca gttgcccggc gttgacgatc cccatatcca tgccattgcg aatagcgtag
     2521 tagaggaaca ccgcgtgaat ggcttcgcgc accggatcgt tgccacgaaa cgagaacgac
     2581 acgttagaaa cgccgccgga aatcagtgcg tgcggcagtt cacgtttgat gtcttcacac
     2641 gcgccgataa agtcctgtgc gtaattgttg tgctcttcaa tgccagttgc gaccgcgaag
     2701 atgttcgggt cgaagatgat atcttcagcc gggaagccaa cctcttcggt gaggattttg
     2761 tacgcccgac ggcaaatctc gattttccgt gcgcgggtat cggcctggcc ctgttcgtca
     2821 aaagccatta ccacgactgc cgcaccgtag cgacgcaaca gtttcgcgtg atggataaag
     2881 gcgtcgacgc cctctttcat cgagatagag ttaacaatgc ctttgccctg gatacacttc
     2941 agaccttttt caataacgtc ccattttgag gagtcgatca ttatcggcac gcgagcgata
     3001 tccggttcac cggcaatcag attgagaaaa cgcaccatcg ccgcttcggc gtcgagcatc
     3061 ccttcatcca tgttgatatc gataatctgc gcaccgtttt ccacctgctg acgcgccaca
     3121 tccagcgcct cgctgtattt ctcttctttg atcagtcgct tgaacttagc ggaaccggtg
     3181 acgttggtgc gttcacccac gttcacaaac aggctatctt cgccaatgtt cagcggctcc
     3241 aggccggaca aacggcaggc tacgggaatt tccggtagtt tgcgcggcgc taatccttct
     3301 actgcacgac tcatcgctgc aatatgttgt ggcgtggtgc cgcagcagcc gccgacgata
     3361 ttgagaaagc ctgcttctgc ccattcacgt atctgttttg ccatcgtgtc ggcgtcgaga
     3421 tcgtattcac caaaggcgtt gggcagcccg gcgttcgggt gggcggtgac gtagcattcc
     3481 gcaatacgcg acagttcctg tacgtactgg cgcagttcgt ctggccccag cgcacagttc
     3541 aggccaaagg tcagcgcttc ggcgtggcgc agggagttgt aaaacgcttc agtagtctgc
     3601 ccggagagcg tgcgcccgga ggcgtcggtg atggtgccgg agatcataat cggcagttca
     3661 acgcccagcg cttcaaactc cgttttcacc gcaaagaccg ccgctttggc gttaagggtg
     3721 tcgaaaacgg tttcaatcag gatcaggtcc gcgccacctt ccaccagcgc tttggtggat
     3781 tctcgataag ccgccaccag ctggtcaaaa gtgatgttgc ggaatgccgg atcgttgacg
     3841 tccggagaaa tagacgccgt gcggttggtc gggccgagaa caccggcaac gtagcgcggt
     3901 ttctccggcg tgcgggcagt ccactcgtcg gcacaggcgc gcgccagttt cgccgctgca
     3961 aagttgattt ccgccgacag ggattccatc tggtaatccg ccatcgcaat ggtcgtggag
     4021 ttgaaggtgt tggtttcgat gatatccgca cccgcttcaa agtaggcgtt gtggatagcg
     4081 gcgatcactt ccggtttgct gagcaccagc aggtcgttgt tgcctttgag atcgcacggc
     4141 cagtcggcaa aacgctcgcc gcgaaaatcg gcctcgttca gtcgataact ctggatcatg
     4201 gtgcccatac cgccgtccag caccagaata cgttcattta actgcgcacg cagttgttcc
     4261 actttgctgc tcacacttgc tcccgacacg cttaacccag atttaataaa aattcaacaa
     4321 accatactgg cataaacgca tctgtggtaa aagcgaccac cacgcaacat gagatttgtt
     4381 caacattaac tcatcggatc tgttcagcaa ctattgcatt cgctgataat aaaaatgaaa
     4441 atgatttcca cgatacagaa aaaggagact gtcatggtcg cacccattcc cgcaaaacgc
     4501 ggcagaaaac ccgccgttgc caccgcacca gcgactggac aggttcagtc tttaacgcgt
     4561 ggcctgaaat tactggagtg gattgccgaa tccaatggca gtgtggcact cacggaactg
     4621 gcgcaacaag ccgggttacc caattccacg acccaccgcc tgctaaccac gatgcagcaa
     4681 cagggctttg tccgtcaggt cggcgaactg ggtcactggg caatcggcgc acacgccttt
     4741 atggtcggca gcagctttct ccagagccgt aatttgttag cgattgttca ccctatcctg
     4801 cgcaatctaa tggaagagtc tggcgaaacg gtcaatatgg cggttcttga tcaaagcaat
     4861 cacgaagcga ttattatcga ccaggtacag tgtactcacc tgatgcgtat gtccgcgcct
     4921 atcggcggta aattgccgat gcacgcttcc ggtgcgggta aagcctttct ggcccaactg
     4981 agcgaagaac aggtgacgaa gctgctgcac cgcaaagggt tacatgccta tacccacgcg
     5041 acgctggtgt ctcctgtgca tttaaaagaa gatctcgccc aaacgcgcaa acggggttat
     5101 tcatttgacg atgaggaaca tgcgctgggg ctgcgttgcc ttgcagcgtg tattttcgat
     5161 gagcaccgtg aaccgtttgc cgcaatttct atttccggac cgatttcacg tattaccgat
     5221 gaccgcgtga ccgagtttgg cgcgatggtg attaaagcgg cgaaggaagt gacgctggcg
     5281 tacggtggaa tgcgctgact ttttcgcctc tgatttatac gaatcgaacg ctaaaccgct
     5341 gcctgcgccg atacgcataa acatcttcca catgcccttc acgaatgcgg ttttgtagtg
     5401 cgcgccagta atcagcgcgg aacaggtcgg cgtgcatctc ttcaaacagc ggaccaatac
     5461 gcgggtcggc acatagccag tggcgaaact cttccgggaa aacatcgccc ggcgagacgc
     5521 tgtaccacgg ttcgctggca agttcgtcct ccgggtagcg cggcggcggg atgtcgcgga
     5581 aattcacttc cgtcatgtag caaatttcat cgtaatcata aaacaccacg cgcccgtgac
     5641 gggtgacgcc aaagttttta aataacatat ccccagggaa gatgttggca gcggcaagct
     5701 ggcgaatggc attgccgtac tcttcaatag catcacgcaa ttgctgcccc tcaacttgct
     5761 caagccagat attaagcggc accatccgcc gttcgatgta gagatggcga atcacaatct
     5821 gttcgccgag atcggtgatt ttttccgctg cttcctggag cagtaattcc attaatgccg
     5881 gggaaatatg ccgcttctcc agcacaaagt tttcaaactc ctgggtgtcc gccattcggc
     5941 ccacgcgatc atgctctttc accagttggt agcaggcacg aacgtgagcg gcagacatct
     6001 ctttctgcgg cgctaacctg tctttgatga ctttgaatac ccgatcaaaa cccggcaacg
     6061 taaacaccaa catcaccatg ccacgaatac ccggcgcttc aatgaactgc tcattacagc
     6121 cctgtagata aacgagatat tcgcggtagc tttcggtttt agcgtgcttc tggcagccga
     6181 tagccatata caattcagcg gtggttttac ctggcagaat ttcccgtagc cactcgacca
     6241 gcgctgcggg cagcggtgca taaaccataa aataagaacg cgcaaagcca aaaacaatgc
     6301 tcgcttcggc ggtcgtcgtc aggcaggtat caataaataa ctcgccgtcg tccgtctggt
     6361 ggatcggcaa taaaaatggc aacgtgccgg aaggcgttat tagtttgccc accagccagg
     6421 ctgccttatt gcggtaaaac agttcgttcg ccacctgtaa atgactttcc gcgaggttgt
     6481 ctgtccccag cgtttccgtc agatggcgaa cgatgtaatg aatgtcacgg cttttattct
     6541 gccaacgcag acgcagcggc agatcgctga taacgcgcat cagcagagat tcccagccgt
     6601 gatcggggtg aaagtctttc gccagtgggc gtggaatggt acgaaagcgg cgctctggct
     6661 gggagctaaa gataaaaagc cgctcgggag taagcgagcg gtggtcaaat aaccgacagt
     6721 acacggagtt aaaaaagctc tccgcaatct cgaagcgcgg gtaatccggc aacagccgcg
     6781 tgtaatgctc tttgacgcgc agtaaaaatg ccgcgtccgt gctttggccg ttggtaatgc
     6841 agcgcagttg ctccaccacc agacccacgt gatgatcgta aagatggata cggtttttca
     6901 tcgcctgctg gacagcatgc cagtcggcct gttcgaaacg ctgctgcgcc ccggaagtca
     6961 cttcgaggaa tcgaccatac tgagcgtcga agccttgcaa aatggtttga gcaatcagta
     7021 attccaggcc acgcggcatt tagcgccctc atcaggagca gagaattgcc ggatgcggcg
     7081 taaatgcctt atccggccta cagtcagcaa cgtttgtagg ttgcttagaa ctgcgattct
     7141 tcagtggagc cggtcagcgc ggtgactgaa gacgtgccgc cctgaataat ggtcgtcact
     7201 ttatcgaaat aacctgtacc cacttcctgc tggtgagata cgaaggtata gccatctttc
     7261 gcagcggcaa attccggctg ctgcactttc tcaacgtaat gcttcatgcc ttcgccctgg
     7321 gcataggcat ttgccaggtc gaacatgtta aaccacatgc tgtggatgcc tgccaaggtg
     7381 atgaactgga acttgtagcc catatccgac agctgctgct ggaagctggc aatagttttg
     7441 tcgtcgagat ttttctgcca gttgaaggac ggcgagcagt tataagccag cagtttgccc
     7501 ggatatttcg cgtggatagc ttccgcaaag cgacgcgcca gttccagatc cggcgtggag
     7561 gtttcacacc aaaccaggtc agcatacggc gcatacgcca ggccacggct gattgcttgc
     7621 tcaatgcctg catgagtacg gaagaagcct tcactggtac gctcgccggt aataaattcg
     7681 ctgtcatacg ggtcgcaatc ggaggtaatc agatccgccg catcagcatc ggtacgggca
     7741 accagaaggg ttggaacgcc cgtcacgtca gctgccagac gcgccgcgac cagtttctga
     7801 atagcttcct gagttggcac taaaactttg ccgcccatgt gaccgcattt cttcactgat
     7861 gccagctgat cttcgaagtg aactgccgct gcaccggctt caatcatcgc tttcatcagt
     7921 tcaaaggcat tcaggacacc gccaaaaccg gcttccgcat cagcaacgat cggcaggaag
     7981 taatcgacat agcgcggatc gcccggctca atgcccgcgg accattggat ctgatccgca
     8041 cggcggaagg tattgttgat ccgctccacc accgctggca ccgagtttgc cggatagagc
     8101 gactgatccg gatacatgct ggccgccagg ttagcgtccg ccgctacctg ccatcccgac
     8161 agatagaccg cttcaatacc cgctttcgcc tgttgcagcg cctgaccgcc agtcagtgca
     8221 ccgaggctgt tgatgtagcc ttttttcgac tcgccgtgca gcagacgcca cattttcgcc
     8281 gcgcccagtt gcgccagcgt gcattcagga ttgactgaac cgcgtaattt caccacatct
     8341 tccgcgctgt acgggcgagt aatgccttcc caacgcggtt gagtccactc tttctgtaat
     8401 tcttcaattt gttgtgtacg ggttttcatg tgcagatgct ccatagttat gtggtggttt
     8461 acgctaacag gtggtagcct ggcagggtca ggaaatcaat taactcatcg gaagtggtga
     8521 tctgttccat caagcgtgcg gcatcgtcaa aacgcccatg ggagaaacgt tcttcgccca
     8581 gttcgctggc aatgactttc atctcttcgc ccagcatctg gcggaacaag gctttagtca
     8641 ccggtttgcc attgctcaac gttttttgat gatggatcca ctgccagatt gaggtacggg
     8701 aaatttcagc cgtcgccgca tcttccatca ggccataaat cggcacacag ccattgccag
     8761 agatccacgc ttcgatgtac tgcacagcca cgcgaatgtt ggcgcgcata ccttcttcgg
     8821 tgcgttcacc atcacaaggt gccagcagct gatcggcagt aatcggcgcg tcttgttcgc
     8881 gcatcacctc aagctggttt ttgcgggagc cgagaatatc gttgaatacc gccattgccg
     8941 tatcagcaag gcccgggtga gcgatccatg tgccatcgtg accgttattg gcttccagcg
     9001 atttatccgc ttttactttg tcgagcacct ggttattgcg ctcttcatct ttgctcggaa
     9061 taaacgccgc catgccgccc atcgcaaaag caccgcgttt atggcaggtt ttaatcaaca
     9121 ggcgtgagta agcattcagg aagggtttat ccatcgtcac tgcctgtctg tctggcagga
     9181 cgcgatcggg atagtttttc aatgttttga tataactgaa gatgtaatcc caacgaccgc
     9241 agttcagacc aacaatatgg tcacgcagcg cgtgaaggat ttcatccatc tggaacacgg
     9301 cgggcagcgt ttcaatcagc aacgtcgcct tgatggtgcc gcgcggcaga ttaaagcgat
     9361 cttctgcata gctgaagact tcgctccacc aggccgcttc ctgccaggac tgggttttcg
     9421 gcagatagaa atagggaccg ctgccctttg ccaacagtgc ctgatagttg tggaagaaat
     9481 agagcgcaaa atcaaacagg ctaccgggga ttgtctcacc acgccaggtg acatgttttt
     9541 ccggcaagtg cagaccgcgt acccgacaaa tcaaaaccgc tggattgggc ttgagctgat
     9601 aaattttgcc tgcttcattg ctatagctga tggtgccgtt aaccgcatcg cgcaggttaa
     9661 tttgcccatc gatcactttg ttccagtctg gtgccagtga atcttcgaaa tcggccataa
     9721 agactttcac attggcgttg agcgcgttga tcaccatctt gcgctctaca ggtccggtaa
     9781 tctctacgcg acgatcttct aagtccgcag gaatcccgcg aattttccag tcagcatcac
     9841 gaatggaagc tgtttccgaa ataaaatcag gcaacattcc gttatcgata tcctgctgct
     9901 gctgaatacg cgctgccaga agtttattgc gttgtggcgt aaaatgcgtc accagctcag
     9961 tcagaaattc taccgcttcg gcagtaagaa tttgcttctc ctgctcgcca tacggccttg
    10021 taaaagccag ttcatcgatt gttgttgcct gttcagtcat cgtgcagctc ctcgtcatgg
    10081 atccgaaaac ttccccactg tgaacgaagg atcgttgtgc gcttttcgtt cagcacaact
    10141 aagactactc atttaaaatg caaaatcaaa aacaatttcc attttaatta aaa
//
LOCUS       DADSYM010000157         9957 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2415
            SAMN08039579-rid11723173.denovo.159, whole genome shotgun sequence.
ACCESSION   DADSYM010000157 DADSYM010000000
VERSION     DADSYM010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039579
            Sequence Read Archive: SRR6362533
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9957)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9957)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980162.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 03:39:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,484
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 4,347
            CDSs (with protein)               :: 4,347
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 43 of 85
            Pseudo Genes (incomplete)         :: 37 of 85
            Pseudo Genes (internal stop)      :: 19 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9957
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039579-rid11723173.denovo.159"
                     /strain="LGE2415"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8302 N 95.9628 W"
                     /collection_date="2015-08-24"
     gene            138..1916
                     /locus_tag="J1Y83_003710"
     CDS             138..1916
                     /locus_tag="J1Y83_003710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent transcriptional regulator"
                     /protein_id="HBA7631821.1"
                     /translation="MELATTQSVLMQIQPTIQRFARMLASVLQLEVEIVDENLCRVAG
                     TGAYGKFLGRQLSGNSRLLRHVLETKTEKVVTQSRFDPLCEGCDSKENCREKAFLGTP
                     VILQDRCVGVISLIAVTHEQQEHISDNLREFSDYVRHISTIFVSKLLEDQGPGDNISK
                     IFATMIDNMDQGVLVVDADNRVQFVNQTALKTLGVVQNNIIGKPVRFRPLTFESNFTH
                     GHMQHIVSWDDKSELIIGQLHNIQGRQLFLMAFHQSHTSFSVANAPDEPHIEQLVGEC
                     RVMRQLKRLISRIAPSPSSVMVVGESGTGKEVVARAIHKLSGRRNKPFIAINCAAIPE
                     QLLESELFGYVKGAFTGASANGKTGLIQAANTGTLFLDEIGDMPLMLQAKLLRAIEAR
                     EILPIGASSPIQVDIRIISATNQNLAQFIAEGKFREDLFYRLNVIPITLPPLRERQED
                     IELLVHYFLHLHTRRLGSVYPGIAPDVVEILRKHRWPGNLRELSNLMEYLVNVVPSGE
                     VIDSTLLPPNLLNNGTTEQSDVTEVSEAHLSLDDAGGTALEEMEKQMIREALSRHNSK
                     KEVADELGIGIATLYRKIKKYELLNT"
     gene            complement(1956..2435)
                     /gene="xdhC"
                     /locus_tag="J1Y83_003711"
     CDS             complement(1956..2435)
                     /gene="xdhC"
                     /locus_tag="J1Y83_003711"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase iron sulfur-binding
                     subunit XdhC"
                     /protein_id="HBA7631822.1"
                     /translation="MNHSETITIECTINGMPFQLHAAPGTPLSELLREQGLLSVKQGC
                     CVGECGACTVLVDGTAIDSCLFLAAWAEGKEIRTLEGEAKGGKLSHVQLAYAKSGAVQ
                     CGFCTPGLIMATTAMLAKPREKPLTITEIRRGLAGNLCRCTGYQMIVNTVLDCEKTK"
     gene            complement(2432..3310)
                     /gene="xdhB"
                     /locus_tag="J1Y83_003712"
     CDS             complement(2432..3310)
                     /gene="xdhB"
                     /locus_tag="J1Y83_003712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase FAD-binding subunit XdhB"
                     /protein_id="HBA7631823.1"
                     /translation="MFDFASYHRATTLADAITLLADNPQAKLLAGGTDVLIQLHHHND
                     RYRHIVDIHNLAELRGITQAEDGALRIGSATTFTQLIEDPVIQRNLPALCAAAASIAG
                     PQIRNVATYGGNICNGATSADSATPTLIYDAKLELHSPRGVRFVPINGFHTGPGKVSL
                     EHDEILVAFHFPPQPKEHAGSAHFKYAMRDAMDISTIGCAAHCRLDNGNFSELRLAFG
                     VAAPTPIRCQHAEQTAQNAPLNLQTLEAISESVLQDVAPRSSWRASKEFRLHLIQTMT
                     KKVISEAVAAAGGKLQ"
     gene            complement(3321..5618)
                     /gene="xdhA"
                     /locus_tag="J1Y83_003713"
     CDS             complement(3321..5618)
                     /gene="xdhA"
                     /locus_tag="J1Y83_003713"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase molybdenum-binding
                     subunit XdhA"
                     /protein_id="HBA7631824.1"
                     /translation="MEAREATATGESCMRVDAIAKVTGRARYTDDYVMAGMCYAKYVR
                     SPIAHGYAVSINDEQARSLPGVLAIFTWEDVPEIPFATAGHAWTLDENKRDTADRALL
                     TRHVRHHGDAVAIVVARDELTAEKAAQLVSIEWQELPVITSPEAALAEDAAPIHNGGN
                     LLKQSTMSTGNVQQTIDAADYQVQGHYQTPVIQHCHMESVTSLAWMEDDSRITIVSST
                     QIPHIVRRVVGQALDIPWSCVRVIKPFIGGGFGNKQDVLEEPMAAFLTSKLGGIPVKV
                     SLSREECFLATRTRHAFTIDGQMGVNRDGTLKGYSLDVLSNTGAYASHGHSIASAGGN
                     KVAYLYPRCAYAYSSKTCYTNLPSAGAMRGYGAPQVVFAVESMLDDAATALGIDPVEI
                     RLRNAAREGDANPLTGKRIYSAGLPECLEKGRKIFEWEKRRAECQNQQGNLRRGVGVA
                     CFSYTSNTWPVGVEIAGARLLMNQDGTINVQSGATEIGQGADTVFSQMVAETVGVPVS
                     DVRVISTQDTDVTPFDPGAFASRQSYVAAPALHSAALLLKEKIIAHAAVMLHQSAMNL
                     TLIKGHIVLIERPEEPLMSLKDLAMDAFYHPERGGQLSAESSIKTTTNPPAFGCTFVD
                     LTVDIALCKVTINRILNVHDSGHILNPLLAEGQVHGGMGMGIGWALFEEMIIDAKSGV
                     VRNPNLLDYKMPTMPDLPQLESAFVEINEPQSAYGHKSLGEPPIIPVAAAIRNAVKMA
                     TGVAINTLPLTPKRLYEEFHLAGLI"
     gene            6032..6787
                     /locus_tag="J1Y83_003714"
     CDS             6032..6787
                     /locus_tag="J1Y83_003714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311765.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HBA7631825.1"
                     /translation="MSAGRLNKKSLGIVMLLSVGLLLAGCSGSKSSDTGTYSGSVYTV
                     KRGDTLYRISRTTGTSVKELARLNGISPPYTIEVGQKLKLGGAKSSSSTRKSTAKSTT
                     KTASVTPSSAVPKSSWPPVGQRCWLWPTTGKVIMPYSTADGGNKGIDISAPRGTPIYA
                     AGAGKVVYVGNQLRGYGNLIMIKHSEDYITAYAHNDTMLVNNGQSVKAGQKIATMGST
                     DAASVRLHFQIRYRATAIDPLRYLPPQGSKPKC"
     gene            6866..6939
                     /locus_tag="J1Y83_003715"
     tRNA            6866..6939
                     /locus_tag="J1Y83_003715"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6898..6900,aa:Gly,seq:ccc)
     gene            7451..8011
                     /locus_tag="J1Y83_003716"
     CDS             7451..8011
                     /locus_tag="J1Y83_003716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001558205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA7631826.1"
                     /translation="MGWSSLVRITIGTFLLAGLSFSTYAKNCKKGIPCGNSCIAVGKT
                     CRIGSSTSSAYKNYSYSYPSSSLHSSIPNSSSHSPSAKVEGKNTASTAKTYLCQYAIA
                     AVQNGRIGSLRVLGSAEVTLHVDSFKANRLNGTYLLSPKLKPDGKLMTADDKSKVYAY
                     DSLLNNFAISDRVARTTEQWDHCRQK"
     gene            8027..8662
                     /locus_tag="J1Y83_003717"
     CDS             8027..8662
                     /locus_tag="J1Y83_003717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000534365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA7631827.1"
                     /translation="MGSELISGLVGALIGGLFTLWGTLIDGRRQDRASFNEAKDKQKS
                     VLIGIKAEVETLLELYKARIYDSLENYEGGKPFESIFPLTQNYFSFYESNSFALTSVN
                     EETMKAIVICYGSARSLVDTFRMNNQVLEDLAKLQTIFHETQKDIHRFNLEQTYHIAT
                     QYGEGLKQIHGEVMSRIAICLDKINVNLDELEKPEQVTRLKTVLRALAFIK"
     gene            8685..9104
                     /locus_tag="J1Y83_003718"
     CDS             8685..9104
                     /locus_tag="J1Y83_003718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000017243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA7631828.1"
                     /translation="MSGVLASTAAAVGIIDKAVGIAKKLADDGGELDKATLKLELANL
                     MSELASVKMEVITTQALLFEAEQKNKQLEEQLKDKHTFTFKDGLYWKEGDETAYCPKC
                     FEVDKVQTHMVFSRAVGQYSASWYCINCRNSIYPKSR"
     gene            complement(9319..9897)
                     /locus_tag="J1Y83_003719"
     CDS             complement(9319..9897)
                     /locus_tag="J1Y83_003719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003863463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HBA7631829.1"
                     /translation="MKGISQALKNVDEVKTVAAELQRNSKTNLFYCLWVFMYESGLRV
                     SDVLKLKYQDVEGQSHLKIKQEKTGNVVRPKVTPAMLSVVAQRKAEQTKMPVATDFIF
                     SAGTLRSKGEAVTRNTVFTEFGKMGRRAGFSNVGCHTPRKSRGRVMFDAGVAIETIAH
                     FLGQTDPRSTMYYIGYGQDVQDDLAEEFSLEW"
BASE COUNT         2568 a         2282 c         2442 g         2665 t
ORIGIN      
        1 tccataattt tattagaaag tgataaaaat cttatgtttt tatcaaagcc aatttcgcct
       61 gagttaatat catcatgatc tgtgaaccat caacgtcttc aatgatcgac ttttaagtaa
      121 gtctggaagg taaagctatg gagcttgcga ctacgcagtc agtattgatg caaattcaac
      181 cgacaattca gcgttttgcc agaatgcttg ccagcgtttt gcagcttgag gttgagatcg
      241 ttgatgaaaa cttgtgtcgc gtcgccggaa cgggcgcgta tgggaagttt cttggccgcc
      301 agttgagcgg caactcacgc ctgctccgcc acgtcctgga aacgaaaact gaaaaagttg
      361 tgacacagtc tcgcttcgat cccctttgtg aaggttgcga tagtaaagaa aattgccgcg
      421 aaaaagcatt tctgggtacg cctgtcattt tacaggatcg ttgtgttggg gtgataagtt
      481 tgattgccgt tacccacgag caacaagaac atatcagtga taatttacgc gaattttccg
      541 attacgttcg ccatatatcc accatttttg tttctaaact tctggaggat cagggaccag
      601 gagataacat cagtaaaata ttcgcgacca tgatcgataa tatggatcag ggcgtgttag
      661 tggttgatgc tgataatcgg gttcagtttg ttaatcagac agctttaaaa acactcggtg
      721 ttgtacaaaa taatattatt ggaaaacctg ttcgtttcag accattaaca tttgagagta
      781 attttaccca tggtcatatg caacatattg tttcgtggga tgataaaagt gaattaatca
      841 ttggtcagtt gcataacatc cagggccgac aactattttt aatggcgttt caccaatcgc
      901 ataccagttt ttctgtagca aatgcacctg atgaaccgca tattgaacaa ttggttggcg
      961 agtgccgtgt tatgcggcaa ttaaaacgac tcattagccg tattgcaccc agcccatcca
     1021 gcgttatggt ggttggtgaa agcggcacgg gtaaagaagt cgtcgcccgt gcaatccata
     1081 agttgagcgg aagacggaat aaacccttta ttgctatcaa ctgtgccgcg attccggaac
     1141 agcttctgga gagcgaactg ttcggttatg ttaaaggcgc atttactggc gcttctgcca
     1201 acggtaaaac agggttgatt caggcggcga atacgggcac gctgtttctc gatgaaattg
     1261 gtgatatgcc attaatgttg caggctaaat tactgcgcgc tattgaggcg cgtgaaattc
     1321 tgccgattgg tgccagtagc ccaatacaag tcgacatccg catcatttct gcaactaatc
     1381 agaatttggc ccagttcatt gccgaaggta aattccgcga agatctcttc taccgactta
     1441 atgttatccc gataactctt ccaccgctgc gtgaacgtca ggaagatatt gaattactgg
     1501 tgcattactt tttacatctg catacccgtc gtctgggatc ggtttatcct ggcattgctc
     1561 ctgatgtcgt cgaaatattg cgtaagcatc gttggcccgg aaacctgcgc gagttaagca
     1621 atttgatgga atatctggtt aacgttgttc cttcaggtga agttatcgat agcacgctat
     1681 tgccgccaaa tctgctgaat aatggcacaa cggagcaaag tgatgtaaca gaggtcagtg
     1741 aggcgcacct gtcactcgat gatgcgggcg gcacggcgct ggaggagatg gaaaagcaaa
     1801 tgatccgcga ggcgctttca cgtcataaca gcaagaagga agttgctgat gaactgggca
     1861 tcggcattgc tacgctctat cgcaagatta agaaatatga gttgttaaac acataacctc
     1921 tgagaatccg gcctgaattc aggccggata tccttttact tcgttttctc gcaatccaga
     1981 actgtattta caatcatctg ataccccgtg cagcgacaaa gatttcccgc cagtccacga
     2041 cgaatttccg taatggttaa tggtttttcg cgtggttttg ccagcatcgc cgtggtagcc
     2101 ataatcaggc ccggcgtaca aaacccgcat tgcactgcgc cggatttcgc ataagccagt
     2161 tggacatgag aaagttttcc acctttcgct tcaccttcca gcgtgcggat ctcttttcct
     2221 tcagcccagg ccgcaaggaa taagcaactg tctattgctg tgccgtcgac cagcaccgta
     2281 caggcaccgc attcgcctac gcagcaccct tgtttgacac ttagcagtcc ttgttcgcgg
     2341 agtaattccg agagcggcgt gcctggtgcg gcgtgaagct gaaaaggcat cccgttaatg
     2401 gtgcattcga tggtaattgt ttcgctgtga ttcattgcaa tttccccccc gccgcggcga
     2461 cggcttcgct aatcactttt ttggtcatcg tctggatgag atgcagacga aactctttac
     2521 tggcccgcca tgaagaacgc ggggcgacat cttgcaggac tgattcgctg atggcttcca
     2581 gcgtttgcag gtttaatggc gcattttgtg cagtctgttc ggcatgttgg cagcgaatcg
     2641 gcgttggcgc ggcaacacca aatgccaggc gtaattcgct gaaattgccg ttatccagtc
     2701 ggcaatgtgc ggcgcagcca attgttgaaa tatccattgc gtcgcgcatg gcatatttaa
     2761 aatgtgcgct gcccgcgtgt tctttcggct gtggcggaaa atgaaaggcg acaaggattt
     2821 cgtcatgctc aagagacact ttgcccggcc cggtgtgaaa gccattaatc gggacgaaac
     2881 gaacaccgcg tggggagtgg agctccagtt tcgcgtcata aattagcgtt ggcgtggcag
     2941 aatctgcgct ggtggcaccg ttgcaaatat ttccgccgta ggtggcgaca ttacggatct
     3001 gcggcccggc gattgatgca gccgcagcac ataacgccgg gagattgcgt tggattacgg
     3061 gatcttcaat gagctgagta aatgttgtcg cggagccgat tcgcagcgcg ccatcttccg
     3121 cctgtgttat tccccgcagc tctgccagat tgtggatatc aacaatatgg cgatagcggt
     3181 cattgtgatg gtgaagctgt atcagtacgt cagtgccacc ggcaagcaat ttggcctgcg
     3241 gattgtcagc cagcagggtg atggcatcgg caagggtggt tgcgcgatgg taagaagcaa
     3301 aatcaaacat gatgttatcc tcaaatcaat cctgccagat ggaattcttc atataatcgt
     3361 tttggcgtta gcggcagtgt attgattgca acaccggtag ccatcttcac cgcgttacga
     3421 atagcagcgg ctacaggaat tatggggggc tcacccagtg acttatgtcc gtatgcggat
     3481 tgcggctcat tgatttcgac gaacgcgctt tccagttgtg gcagatccgg catggtcggc
     3541 attttgtaat ccagcagatt ggggttacgg accacgccgc ttttcgcatc gatgatcatc
     3601 tcttcaaata gcgcccagcc aatgcccatt cccattccgc cgtgtacctg accttctgcc
     3661 agcagcggat taagaatatg gcccgaatca tgaacgttga ggatgcggtt gatggtgact
     3721 ttgcacagtg caatatcgac cgtcagatca acaaaggtac agccaaacgc cggtgggtta
     3781 gtggtggttt tgatggagct ttcggcagag agctgcccgc cgcgttcagg gtggtagaaa
     3841 gcgtccatcg ccaaatcttt taacgacatt aacggttctt ccggtctttc aatcagcacg
     3901 atgtggcctt ttatcagggt cagattcatc gctgactgat gtagcatgac tgcggcgtga
     3961 gcgatgattt tctcttttaa taacagtgcc gcactgtgca gcgcaggcgc ggcaacatag
     4021 ctctgacgtg aggcaaatgc gccggggtcg aatggtgtaa cgtcggtgtc ttgtgtcgaa
     4081 attacgcgga catcgctgac cggaactccc acggtttctg ccaccatttg cgagaacacg
     4141 gtgtcggcac cctggccgat ttccgtcgcg ccgctttgca cgttgatggt tccatcctga
     4201 ttcatcaaca ggcgcgcgcc tgctatttct acgccgacag gccaggtgtt agaggtgtag
     4261 ctaaaacagg cgacgccaac gccacgacgt aaattgcctt gctggttctg gcactctgca
     4321 cggcgttttt cccattcaaa gattttccgg cctttttcaa gacactccgg caaccctgcg
     4381 ctgtaaatac gttttcccgt gagcggatta gcatctcctt cgcgggcggc gttgcgtaaa
     4441 cgaatttcaa caggatcaat acctaacgct gtcgcggcat catcaagcat agactcaacg
     4501 gcaaatacga cttgtggcgc gccataacca cgcatcgcac cagccgaggg gaggttggta
     4561 tagcaggtct ttgaactgta agcgtaggca caacgaggat aaaggtaagc gactttattc
     4621 cccccagcag aagcaatgga gtgcccgtga gatgcataag cgccggtgtt agacagaaca
     4681 tccagactat aacctttcaa tgttccgtcg cggttcacgc ccatttgccc gtcaatagta
     4741 aaagcgtggc gggtacgggt tgcgaggaaa cactcttcac ggctaaggga aactttcacc
     4801 ggaatgccgc caagtttgct ggtcaggaat gccgccattg gctcttccag tacatcctgt
     4861 ttattaccaa aaccgccacc gataaacggt ttgatgactc gtacgcatga ccagggaata
     4921 tccagcgcct gaccaaccac gcggcgaaca atgtgcggga tctgggtgct ggaaacgatg
     4981 gtaattcgcg agtcatcctc catccatgcc agcgatgtca cgctttccat atgacaatgt
     5041 tgaataacgg gagtctgata gtgcccctgt acctggtagt cggcggcatc gattgtttgt
     5101 tggacattac ccgtcgacat cgtgctttgt ttcagtaaat tgccaccgtt atggattggt
     5161 gcagcgtctt ctgccagcgc cgcttctggc gaggtgataa cgggtaattc ttgccactca
     5221 atgctgacca attgcgccgc tttttctgcc gtgagttcat cgcgggccac gacgatggca
     5281 acggcgtcgc catgatgacg aacatggcga gttagcagtg cgcgatcggc ggtatcgcgc
     5341 ttgttttcgt caagtgtcca ggcatgccct gccgtggcga atgggatttc tggcacatct
     5401 tcccaggtaa aaatcgccag gacgcccggc aaactcctgg cttgttcatc attaatactt
     5461 acagcataac catgtgcgat agggctacgt acatatttcg cgtaacacat gcccgccata
     5521 acataatcgt cagtatatcg tgcccgcccg gtgaccttag caatggcatc gacgcgcatg
     5581 catgattcac ccgtagcggt tgcttcccgc gcttccatag aaatcccctc gatttgaaca
     5641 tttggttctt atggcaaaga tttagtctaa gcaggcaaga tttacgccag aaacgacgat
     5701 aagccttcgg ggaggaagga agtgtgatga cgttaaaaaa acacagatat gatacgaaac
     5761 aggactctga atagggacag tgatacgtat caatatgagt ggcgataaaa tcaatgtgat
     5821 aaattaggat tattacgcat caaggtggga aatagggtaa aagtcaggaa cttcgatacc
     5881 actacagcaa atattttgct gatgatactt ccctttgcca aatgaaatta acctctttct
     5941 ctattacatc gtttttccag aagcaagaga ttgggtatca agaggctggc tgctatgata
     6001 tggcgtcttg ttttttaagc gaggaaagat tttgagtgcg ggacgcctga ataaaaaatc
     6061 tctgggtatc gtgatgttgt tatcggttgg actgcttttg gcgggctgtt cgggtagcaa
     6121 atcatccgat acaggaacgt attccggctc cgtttacacc gtgaaacggg gggatacgct
     6181 atatcgtatt tcgcgcacca cgggaaccag cgtaaaagaa ctggcgcgac tgaacggcat
     6241 ttccccccct tacaccattg aagtaggtca gaaactaaaa ctgggtgggg cgaaaagtag
     6301 cagtagtaca cgtaaatcaa ccgccaaatc aacgaccaaa accgcatcgg ttacaccgtc
     6361 atcagcggta ccgaaatctt cctggccgcc agtagggcaa cgttgttggt tatggccaac
     6421 gacagggaaa gttatcatgc cgtattcgac agctgatggc ggcaataaag ggattgatat
     6481 ctcagctcca cggggtacac ctatttacgc cgcgggtgca ggaaaggtgg tgtatgtggg
     6541 caaccagctg cgtggctatg gtaatctcat catgattaaa cacagtgaag attacattac
     6601 ggcttacgct cataatgaca cgatgctggt aaataatggg caaagcgtga aggctgggca
     6661 aaaaatcgct accatgggga gcactgatgc ggcatctgtt cgcctgcatt tccagattcg
     6721 ttaccgtgca acggcaattg atccgctacg ttacttgccg cctcagggca gcaagccaaa
     6781 atgctgatgg cgaattaatc agcagtcagc agcgtggtac ttgctaagga gagcgtaagg
     6841 tttataatgc cttacgcatc tcgaagcggg cgtagttcaa tggtagaacg agagcttccc
     6901 aagctctata cgagggttcg attcccttcg cccgctccag aatctcccca ggtggagaag
     6961 acaaaaaaag tcttctctgt cagcaactta aacagttacc tatttaattt gcttaggagt
     7021 tctaccattg tgatccgccc ttagtaactg tcacgttaag ttacacggtg agatgacagt
     7081 ataacctaac gttggcggca gttgcacctc tcacaccgcg atagcacaac gtgaagataa
     7141 agcccatcct ggcattgtaa tgttatcaac agactgaaca cacgcattta agcccgatgt
     7201 gcggccttgt ggctgcgtaa acaggtttat ggtgatattg gtccttgcaa accatatgca
     7261 agctctatca gcgtttcagc cacgctccca acgggtacac ggttatcctg ataaggtgtg
     7321 atctttctga caatctgatg aagtcgctac gctccgcttg ttgctgtatc accaacgggg
     7381 ctttatggtt cctattccct taatttaggt atggtatcat gtagggaaaa actaaaaaga
     7441 agggggggtt atgggttggt cttcgctggt taggataacc attggtacat ttctattagc
     7501 tgggttgtcg ttctctacat acgcgaagaa ctgcaagaaa ggtataccat gcggtaattc
     7561 gtgcattgcg gttggaaaaa cgtgcaggat tggatcaagc acctcttctg cgtataaaaa
     7621 ttattcttac tcttacccat catcttctct tcatagttca atccctaact cctcttcaca
     7681 ttccccatca gcgaaggttg agggaaaaaa cacagcttca actgcgaaaa cctatctctg
     7741 tcagtacgct attgcagcag tacagaatgg tagaataggt tctttacgtg tgctgggaag
     7801 tgctgaagta acgttgcatg ttgatagctt caaagctaac cgtttaaacg gtacatactt
     7861 gctttcgcca aagttgaaac ctgatggtaa gttgatgaca gcagatgata aaagcaaagt
     7921 gtacgcttat gattcgttgc tgaataactt tgcaatcagt gatcgagttg caaggactac
     7981 tgagcaatgg gatcattgcc gtcaaaaatg attgttaggg ggcaacatgg gaagtgaact
     8041 gatttctggt ttagttggtg ccttgatagg gggattgttc accctgtggg gtactctgat
     8101 cgatggacgt aggcaagaca gagcttcatt taatgaagca aaggataagc aaaagagtgt
     8161 cttgattgga ataaaagcag aagtcgaaac gcttctggaa ctttataaag ccagaattta
     8221 cgattctctt gaaaattatg aaggtggtaa accttttgaa agcatctttc cgttgactca
     8281 aaattatttc tcattctatg aaagcaactc ttttgcatta acttctgtta atgaagagac
     8341 aatgaaggca attgttattt gttatggctc agcaaggagt ctggttgata ctttcagaat
     8401 gaataatcag gttttagagg acttagctaa acttcaaacc atatttcatg aaacgcagaa
     8461 agatatccat cgttttaatt tagaacagac ttaccatatt gccactcaat atggcgaagg
     8521 tttgaagcag attcatggcg aggttatgtc acgtatagcc atctgtttag ataaaattaa
     8581 tgttaatttg gatgaacttg aaaagcctga gcaggtaacg cggttaaaaa ccgttcttag
     8641 ggctttagct tttataaagt gaacttgtta aaattagggg caatatgtct ggagtgttgg
     8701 caagtacagc ggcggctgta ggtatcattg acaaagctgt aggtattgca aagaagctcg
     8761 ctgatgatgg cggcgaactt gataaagcaa cgcttaaact tgagcttgct aacctcatga
     8821 gcgaacttgc aagtgtgaag atggaagtaa tcacaaccca agcacttctt tttgaggctg
     8881 aacagaaaaa taagcagctt gaagagcagc taaaggataa gcacaccttc actttcaaag
     8941 atggacttta ctggaaagaa ggcgatgaaa cagcctactg tcccaaatgc tttgaagttg
     9001 ataaggtgca aacacacatg gttttttcgc gtgccgttgg tcaatactcc gcgagttggt
     9061 attgcattaa ttgcagaaac agcatttatc caaaatcccg ttaaaatcgt tattctttgg
     9121 cggaagtgtc aaatgttttt gcataagggc atttcccctt agttacgtaa gtgaacagat
     9181 aattgctgtt ctgatacgtg cacagcgttt ttccattctc cgtctgttct tcctgtgggt
     9241 tgaccattgt tatctgtccg gcattcgata ggacaggcaa tagcgccaac gtcagcacaa
     9301 ataagctttt aatttggatc accattctaa agaaaactcc tctgctaaat cgtcctgcac
     9361 atcctgacca tacccgatgt aatacatagt agagcgcgga tctgtctgtc ccaggaaatg
     9421 ggctattgtc tctatagcta cccctgcatc aaacatcaca cggccccgac ttttacgtgg
     9481 cgtatggcat cccacattag agaatccggc tctacgtccc atcttcccaa attcagtgaa
     9541 taccgtgtta cgggttacgg cttcgccttt actgcgtaat gtgccagcac tgaaaataaa
     9601 atctgtagct acaggcatct tcgtttgctc tgccttgcgt tgtgccacca ctgacaacat
     9661 ggcgggggta acttttggcc ttactacgtt tccggttttc tcctgcttta tcttcaaatg
     9721 agattgcccc tcaacatcct gatattttag cttgagtacg tcagagacac gcaacccgct
     9781 ttcatacata aacacccata agcaataaaa caggttggtt ttactattgc gctgtagctc
     9841 tgctgctact gtttttacct cgtctacatt cttgagggct tggctaatac ctttcatctt
     9901 tatactctcc ttattcgttt caatgaaagt agttttgatc ataaaatgat cattttc
//
LOCUS       DADSYM010000158         9871 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2415
            SAMN08039579-rid11723173.denovo.160, whole genome shotgun sequence.
ACCESSION   DADSYM010000158 DADSYM010000000
VERSION     DADSYM010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039579
            Sequence Read Archive: SRR6362533
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9871)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9871)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980162.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 03:39:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,484
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 4,347
            CDSs (with protein)               :: 4,347
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 43 of 85
            Pseudo Genes (incomplete)         :: 37 of 85
            Pseudo Genes (internal stop)      :: 19 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9871
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039579-rid11723173.denovo.160"
                     /strain="LGE2415"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8302 N 95.9628 W"
                     /collection_date="2015-08-24"
     gene            complement(221..2794)
                     /gene="clpB"
                     /locus_tag="J1Y83_003720"
     CDS             complement(221..2794)
                     /gene="clpB"
                     /locus_tag="J1Y83_003720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002461515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HBA7631830.1"
                     /translation="MRLDRLTNKFQLALADAQSLALGHDNQFIEPLHLMSALLNQEGG
                     SVSPLLTSAGINAGQLRTDINQALNRLPQVEGTGGDVQPSQDLVRVLNLCDKLAQKRG
                     DNFISSELFVLAALESRGTLADILKAAGATTANITQAIEQMRGGESVNDQGAEDQRQA
                     LKKYTIDLTERAEQGKLDPVIGRDEEIRRTIQVLQRRTKNNPVLIGEPGVGKTAIVEG
                     LAQRIINGEVPEGLKGRRVLALDMGALVAGAKYRGEFEERLKGVLNDLAKQEGNVILF
                     IDELHTMVGAGKADGAMDAGNMLKPALARGELHCVGATTLDEYRQYIEKDAALERRFQ
                     KVFVAEPSVEDTIAILRGLKERYELHHHVQITDPAIVAAATLSHRYIADRQLPDKAID
                     LIDEAASSIRMQIDSKPEELDRLDRRIIQLKLEQQALMKESDEASKKRLDMLNEELSD
                     KERQYSELEEEWKAEKASLSGTQTIKAELEQAKIAIEQARRVGDLARMSELQYGKIPE
                     LEKQLEAATQLEGKTMRLLRNKVTDAEIAEVLARWTGIPVSRMMESEREKLLRMEQEL
                     HHRVIGQNEAVDAVSNAIRRSRAGLADPNRPIGSFLFLGPTGVGKTELCKALANFMFD
                     SDEAMVRIDMSEFMEKHSVSRLVGAPPGYVGYEEGGYLTEAVRRRPYSVILLDEVEKA
                     HPDVFNILLQVLDDGRLTDGQGRTVDFRNTVVIMTSNLGSDLIQERFGELDYAHMKEL
                     VLGVVSHNFRPEFINRIDEVVVFHPLGEQHIASIAQIQLKRLYKRLEERGYEIHISDE
                     ALKLLSENGYDPVYGARPLKRAIQQQIENPLAQQILSGELVPGKVIRLEVNEDRIVAV
                     Q"
     gene            complement(2924..3655)
                     /gene="pgeF"
                     /locus_tag="J1Y83_003721"
     CDS             complement(2924..3655)
                     /gene="pgeF"
                     /locus_tag="J1Y83_003721"
                     /EC_number="1.10.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphenol oxidase"
                     /protein_id="HBA7631831.1"
                     /translation="MSKLIVPQWPLPKGVAACSSTRIGGVSLPPYDSLNLGAHCGDNP
                     DHVEENRKRLFAAGNLPSKPVWLEQVHGKDVLKLTGEPYASKRADASYSNTPGTVCAV
                     MTADCLPVLFCNRAGTEVAAAHAGWRGLCAGVLEETVSCFADNPENILAWLGPAIGPR
                     AFEVGAEIREAFMAVDAKASTAFIQHGDKYLADIYQLARQRLASVGVEQIFGGDRCTY
                     TENETFFSYRRDKTTGRMASFIWLI"
     gene            complement(3652..4632)
                     /gene="rluD"
                     /locus_tag="J1Y83_003722"
     CDS             complement(3652..4632)
                     /gene="rluD"
                     /locus_tag="J1Y83_003722"
                     /EC_number="5.4.99.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017383977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(1911/1915/1917) synthase
                     RluD"
                     /protein_id="HBA7631832.1"
                     /translation="MAQRVQLTATVSENQLGQRLDQALAEMFPDYSRSRIKEWILDQR
                     VLVNGKVCDKPKEKVLGGEQVAINAEIEEEARFEPQDIPLDIVYEDEDIIVINKPRDL
                     VVHPGAGNPDGTVLNALLHYYPPIADVPRAGIVHRLDKDTTGLMVVAKTVPAQTRLVE
                     SLQRREITREYEAVAIGHMTAGGTVDEPISRHPTKRTHMAVHPMGKPAVTHYRIMEHF
                     RVHTRLRLRLETGRTHQIRVHMAHITHPLVGDPVYGGRPRPPKGASEAFISTLRKFDR
                     QALHATMLRLYHPISGIEMEWHAPIPQDMVELIEVMRADFEEHKDEVDWL"
     gene            4767..5504
                     /gene="bamD"
                     /locus_tag="J1Y83_003723"
     CDS             4767..5504
                     /gene="bamD"
                     /locus_tag="J1Y83_003723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HBA7631833.1"
                     /translation="MTRMKYLVAATTLSLFLAGCSGSKEEVPDNPPNEIYATAQQKLQ
                     DGNWRQAITQLEALDNRYPFGPYSQQVQLDLIYAYYKNADLPLAQAAIDRFIRLNPTH
                     PNIDYVMYMRGLTNMALDDSALQGFFGVDRSDRDPQHARAAFSDFSKLVRGYPNSQYT
                     TDATKRLVFLKDRLAKYEYSVAEYYTERGAWVAVVNRVEGMLRDYPDTQATRDALPLM
                     ENAYRQMQMNAQAEKVAKIIAANSSNT"
     gene            5774..6115
                     /gene="raiA"
                     /locus_tag="J1Y83_003724"
     CDS             5774..6115
                     /gene="raiA"
                     /locus_tag="J1Y83_003724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HBA7631834.1"
                     /translation="MTMNITSKQMEITPAIRQHVADRLAKLEKWQTHLINPHIILSKE
                     PQGFVADATINTPNGVLVASGKHEDMYTAINELINKLERQLNKLQHKGEARRAATSVK
                     DANFVEEVEEE"
     gene            6219..6266
                     /locus_tag="J1Y83_003725"
     CDS             6219..6266
                     /locus_tag="J1Y83_003725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA7631835.1"
                     /translation="MKHTPFFFAFFFTFP"
     gene            6365..7525
                     /gene="pheA"
                     /locus_tag="J1Y83_003726"
     CDS             6365..7525
                     /gene="pheA"
                     /locus_tag="J1Y83_003726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional chorismate mutase/prephenate
                     dehydratase"
                     /protein_id="HBA7631836.1"
                     /translation="MTSENPLLALREKISALDEKLLALLAERRELAVEVGKAKLLSHR
                     PVRDIDRERDLLERLITLGKAHHLDAHYITRLFQLIIEDSVLTQQALLQQHLNKINPH
                     SARIAFLGPKGSYSHLAARQYAARHFEQFIESGCAKFADIFNQVETGQADYAVVPIEN
                     TSSGAINDVYDLLQHTSLSIVGEMTLTIDHCLLVSGTTDLSAINTVYSHPQPFQQCSK
                     FLNRYPHWKIEYTESTSAAMEKVAQAKSPHVAALGSEAGGTLYGLQVLERIEANQRQN
                     FTRFVVLARKAINVSDQVPAKTTLLMATGQQAGALVEALLVLRNHSLIMTRLESRPIH
                     GNPWEEMFYLDIQANLESAEMQKALKELGEITRSMKVLGCYPSENVVPVDPT"
     gene            complement(7568..8689)
                     /gene="tyrA"
                     /locus_tag="J1Y83_003727"
     CDS             complement(7568..8689)
                     /gene="tyrA"
                     /locus_tag="J1Y83_003727"
                     /EC_number="1.3.1.12"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional chorismate mutase/prephenate
                     dehydrogenase"
                     /protein_id="HBA7631837.1"
                     /translation="MVAELTALRDQIDEVDKALLNLLAKRLELVAEVGEVKSRFGLPI
                     YVPEREASMLASRRAEAEALGVPPDLIEDVLRRVMRESYSSENDKGFKTLCPSLRPVV
                     IVGGGGQMGRLFEKMLTLSGYQVRILEQHDWDRAADIVADAGMVIVSVPIHVTEQVIG
                     KLPPLPKDCILVDLASVKNGPLQAMLAAHDGPVLGLHPMFGPDSGSLAKQVVVWCDGR
                     KPEAYQWFLEQIQVWGARLHRISAVEHDQNMAFIQALRHFATFAYGLHLAEENVQLEQ
                     LLALSSPIYRLELAMVGRLFAQDPQLYADIIMSSERNLALIKRYYKRFGEAIELLEQG
                     DKQAFIDSFRKVEHWFGDYAQRFQSESRVLLRQANDNRQ"
     gene            complement(8700..9770)
                     /gene="aroF"
                     /locus_tag="J1Y83_003728"
     CDS             complement(8700..9770)
                     /gene="aroF"
                     /locus_tag="J1Y83_003728"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroF"
                     /protein_id="HBA7631838.1"
                     /translation="MQKDALNNVHITDEQVLMTPEQLKAAFPLSLQQEAQIADSRKTI
                     SDIIAGRDPRLLVVCGPCSIHDPETALEYARRFKALAAEVSDSLYLVMRVYFEKPRTT
                     VGWKGLINDPHMDGSFDVEAGLQIARKLLLELVNMGLPLATEALDPNSPQYLGDLFSW
                     SAIGARTTESQTHREMASGLSMPVGFKNGTDGSLATAINAMRAAAQPHRFVGINQAGQ
                     VALLQTQGNPDGHVILRGGKAPNYSPADVAQCEKEMEQAGLRPSLMVDCSHGNSNKDY
                     RRQPAVAESVVAQIKDGNRSIIGLMIESNIHEGNQSSEQPRSEMKYGVSVTDACISWE
                     MTDALLREIHQDLNGQLTARVA"
BASE COUNT         2506 a         2629 c         2317 g         2419 t
ORIGIN      
        1 ctttcctctg ttgagattct caggagaacc ccgctgaccc ggcgacgtgt ttgccgttgt
       61 tccgtgtcag tggtggcgca ttatagggag ttattccggc ctgacaagca aaaaatataa
      121 aaactttatt gttcgctcat ttttcaggca aaacatcttt aatatagtct tttccgtcta
      181 acttatagac aaaaacgagc cccgaaggct cgttttatca ttactggacg gcgacgatcc
      241 ggtcttcatt aacttccagg cgaataactt tacccggaac caattcacca gacagtattt
      301 gctgtgccag cgggttttcg atctgctgct ggatagcacg tttcagagga cgtgcaccat
      361 agaccggatc gtaaccgttc tcgctcagca gtttcagcgc ctcgtcagaa atgtggattt
      421 cataaccacg ttcttccaga cgtttgtaca gacgtttcaa ctgaatctgc gcaatcgacg
      481 caatgtgttg ttcacccagc ggatggaaga cgacgacttc atcgatacgg ttaatgaatt
      541 ccggacggaa gttatggctt accacaccga gcaccagctc tttcatgtgc gcataatcca
      601 gttcaccaaa acgttcctga atcagatcgg aaccgaggtt agaggtcata atgacgaccg
      661 tattacggaa gtcgaccgtt ctcccctgcc cgtcagtcag acgcccatca tccagtacct
      721 gcaacagaat gttgaagaca tccggatgcg ctttttccac ttcatccagc aggatgacgg
      781 aatacggacg acgacgcacc gcttcggtca ggtagccacc ttcttcataa ccgacatatc
      841 ccggaggcgc accaaccaga cgagacaccg agtgtttctc cataaactcg gacatatcga
      901 tacggaccat cgcctcgtcg ctatcaaaca taaagttcgc cagcgcctta caaagctctg
      961 ttttccccac acccgttggg ccgaggaaca ggaatgaacc aatcgggcga tttggatccg
     1021 ccagccccgc acggctacga cgaatagcgt tagataccgc atcaaccgct tcgttctgac
     1081 caataacgcg atggtgcagt tcttgctcca tacgcagcag tttttcgcgc tcgctttcca
     1141 tcatgcgaga aaccggaatt cccgtccaac gcgccagcac ttcagcaatt tcggcatcgg
     1201 tcactttatt acgcaacaga cgcatagttt tgccttcgag ctgcgttgcg gcttccagtt
     1261 gcttttccag ttccgggatt ttgccgtatt gcagttcaga catccgcgcc aggtcaccca
     1321 cacggcgagc ctgttcaata gcgattttcg cctgttccag ttccgcttta atggtctgcg
     1381 taccagaaag cgaggctttc tctgctttcc actcttcttc taactcggag tactgacgtt
     1441 ctttgtcgct cagttcttcg ttgagcatat ccaggcgttt tttactggct tcatcagact
     1501 ctttcattaa cgcctgttgt tccagtttga gctggatgat acgacgatcg agtcggtcga
     1561 gttcttctgg ttttgagtca atctgcatac gaatgctgga tgctgcttca tcgatcaggt
     1621 cgatggcttt atccggcagc tgacggtcag caatgtagcg atgagacaac gtcgctgctg
     1681 caacaattgc cgggtcagta atttgcacat ggtggtgcag ctcgtaacgt tctttcaggc
     1741 cacgcagaat agcaatggta tcttcaacag aaggctcagc aacaaacact ttctggaaac
     1801 gacgttccag cgcagcatct ttttcgatgt actggcgata ttcgtcaagc gtcgtggcac
     1861 ctacgcagtg caattcacca cgcgccagtg caggtttcag catgtttccg gcgtccattg
     1921 cgccatcggc tttacccgcg ccgaccatgg tatgtaattc gtcgataaac aggatgacgt
     1981 tgccttcctg tttagcaaga tcgttaagta cgccttttaa acgttcttca aactcaccgc
     2041 gatatttcgc cccagccacc agcgcgccca tatccagcgc cagtacgcgg cggcctttca
     2101 acccttcggg cacttcgccg ttgataatac gctgcgccag accttcaacg atggcagttt
     2161 taccgacgcc gggttcacca atcaataccg ggttattttt agtacgacgt tgcagcacct
     2221 gaatggtacg gcgaatttct tcatcacgac caatcaccgg atcgagtttg ccttgttcgg
     2281 ctcgttcggt aaggtcgatg gtatattttt tcaaagcctg acgttggtct tcagcacctt
     2341 gatcgttcac gctttcacct ccacgcattt gttcaatcgc ttgagtaatg ttggcggtgg
     2401 tcgcccctgc tgctttcagg atgtcggcca gcgtgccgcg agactcaagt gccgccagaa
     2461 cgaacagttc tgacgagata aagttatcac cacgtttttg cgccagcttg tcgcaaagat
     2521 taagaacgcg caccagatcc tgtgatggct ggacatcacc accagtacct tcaacctgcg
     2581 gtaaacgatt taatgcctga ttgatatctg tgcgcaactg gccagcattt atgccagcgg
     2641 atgttaataa aggactaacc gaaccccctt cctgattcag cagggcgctc attaaatgaa
     2701 gtggttcgat gaattggttg tcgtgcccga gtgcaagtga ttgggcatcg gcaagagcaa
     2761 gctggaattt attagtaaga cgatccagac gcataactcc tcccataacg gatcaaagtt
     2821 gctactggag attaaatgag gtcatccctc aattattcaa ggttattgac cagattaatg
     2881 tgaaaagaaa atcacgcgta ccggatcgtc ttgattcttt aggttatatc agccaaatga
     2941 aacttgccat acgaccggtg gtcttgtcgc gacgataaga gaagaaagtc tcattttccg
     3001 tatatgtaca acggtcgccg ccgaaaattt gttcaacacc aacgctcgcc agacgctgcc
     3061 gggcaagctg ataaatatcc gccagatact tatcaccatg ctgaatgaaa gctgtactcg
     3121 ctttggcgtc tactgccata aacgcctcgc gaatctccgc ccccacttca aacgcgcgtg
     3181 gacctattgc cggccctaac caggcgagaa tattttccgg attatcagca aaacaggaaa
     3241 ccgtctcttc cagcacgcct gcgcacagtc cacgccagcc agcatgagcg gcggcgactt
     3301 ccgttcccgc tcgattgcaa aacagcacag ggaggcaatc ggcagtcatc actgcgcaaa
     3361 ccgtgccggg cgtattgcta taagaggcat ctgctcgttt tgaggcataa ggttcaccag
     3421 taagcttaag cacatctttg ccgtgtacct gctcaagcca gaccggttta gaaggcaaat
     3481 tgcccgcagc aaaaagtcgc ttgcgattct cctcaacgtg atccgggtta tcaccacaat
     3541 gagcgccaag gttgagagag tcgtaaggcg gcaagctcac gccgccgata cgggtagagc
     3601 tacaggccgc aacacctttt ggcagcggcc actgcgggac aatcagctta ctcataacca
     3661 gtccacttca tccttatgct cttcgaaatc ggcgcgcatc acctcaatca gctccaccat
     3721 atcttgtgga ataggcgcat gccattccat ttcgataccg gagatcgggt gataaagacg
     3781 cagcatggtt gcatgaagcg cctggcggtc aaacttacgc agcgtggaga taaacgcttc
     3841 cgaagcacct tttggcggac gcggacggcc accataaacc ggatcaccca ccagcggatg
     3901 agtgatatgg gccatatgca cgcggatctg gtgcgtacgt ccagtttcca gacgcaaccg
     3961 cagacgcgtg tgcacacgga agtgttccat gatgcgatag tgagtgaccg ccggtttacc
     4021 catcggatgc accgccatgt gagtacgttt ggtcgggtgg cggctgattg gttcatctac
     4081 tgtaccgcca gcggtcatat gaccaattgc caccgcttca tactcacgag taatttcacg
     4141 ccgttgcaaa gattcgacta aacgcgtctg agccggaacg gtttttgcca caaccatcag
     4201 gccggtggtg tctttatcca gacgatggac gatgcccgca cgcggtacat cggcaatggg
     4261 cggatagtaa tgaagcaacg cattcagtac cgtaccatcc gggttacccg cgccaggatg
     4321 taccaccagg tcgcgtggtt tattaatgac gatgatatct tcatcttcat agacgatatc
     4381 cagcgggata tcctgcggtt caaaacgcgc ttcttcttca atctcagcgt tgatggccac
     4441 ctgctcgcca cccaatactt tttctttcgg cttatcacac actttgccgt taaccagcac
     4501 gcgctggtcg aggatccatt cttttattcg cgaacgtgaa taatccggga acatttcggc
     4561 caaagcctga tctaagcgtt gaccgagttg gttttcggac accgttgcag tgagctgtac
     4621 tcgttgtgcc atatatactg cttcttcgtt tatcgttggg gttttacggc tttgccgttt
     4681 aatatagtgt gctattgtag ctggtcttaa ccgggagcag gaacagagaa tctcccgtat
     4741 tacattttga ggaaagtcaa aacgtcatga cgcgcatgaa atatctggtg gcagccacca
     4801 cactaagcct gtttttggcg ggttgctcgg ggtcaaagga agaagtacct gataatccgc
     4861 caaatgaaat ttacgcgact gcacaacaaa agctgcagga cggtaactgg agacaggcaa
     4921 taacgcaact ggaagcgtta gataatcgct atccgtttgg tccgtattcg cagcaggtgc
     4981 agctggatct catctacgcc tactataaaa acgccgattt gccgttagca caggctgcca
     5041 tcgatcgttt tattcgcctt aacccgaccc atccgaatat cgattatgtc atgtacatgc
     5101 gtggcctgac caatatggcg ctggatgaca gtgcgctgca agggttcttt ggcgttgacc
     5161 gtagcgatcg cgatcctcaa catgcacgag ctgcgtttag tgacttttcc aaactggtgc
     5221 gcggctatcc gaacagtcag tacaccaccg atgccaccaa acgtctggta ttcctgaaag
     5281 atcgtctggc gaaatatgaa tactccgtgg ccgagtatta tacagaacgt ggtgcatggg
     5341 ttgctgtcgt taaccgcgta gaaggcatgt tgcgcgacta tccggatacc caggctacgc
     5401 gtgatgcgct gccgctgatg gaaaatgcat accgtcagat gcagatgaat gcgcaagctg
     5461 aaaaagtagc gaaaatcatc gccgcaaaca gcagcaatac ataacagaaa cctgaaacac
     5521 aaaacggcag ccttgagctg ccgttttttt attctgttag ttgtgaaact gaagcgattt
     5581 agtcactatc gatctcatca aatatggctc gctttgagat attcctcaag taaaaaaaca
     5641 cctcttcctg cgatttctca caaaaaagat tcgttgacaa aaagtgacaa aattatgaga
     5701 tttccatcac acattttgac atcaggaacg ttatgctgaa ttcaccaaga cgggaagaca
     5761 agaggtaaaa tttatgacaa tgaacattac cagcaaacaa atggaaatta ctccggccat
     5821 ccgccaacat gtcgcagacc gtctcgccaa actggaaaaa tggcaaacac atctgattaa
     5881 tccacatatc attctgtcca aagagccaca agggtttgtt gctgacgcca caatcaatac
     5941 acctaacggc gttctggttg ccagtggtaa acatgaagat atgtacaccg caattaacga
     6001 attgatcaac aagctggaac ggcagctcaa taaactgcag cacaaaggcg aagcacgtcg
     6061 tgccgcaaca tcggtgaaag acgccaactt cgtcgaagaa gttgaagaag agtagtcctt
     6121 tatattgagt gtatcgccaa cgcgccttcg ggcgcgtttt ttgttgacag cgtgaaaaca
     6181 gtacgggtac tgtactaaag tcacttaagg aaacaaacat gaaacacaca ccgtttttct
     6241 tcgcattctt ttttaccttc ccctgaatgg gaggcgtttc gtcgtgtgaa acagaatgcg
     6301 aagacgaaca ataaggcctc ccaaatcggg gggccttttt tattgataac aaaaaggcaa
     6361 cactatgaca tcggaaaacc cgttactggc gctgcgagag aaaatcagcg cgctggatga
     6421 aaaattatta gcattactgg cagagcgacg cgaactggcc gtcgaggtgg gaaaagccaa
     6481 actgctctcg catcgcccgg tacgtgatat tgatcgtgaa cgcgatttgc tggaaagatt
     6541 aattacgctc ggtaaagcgc accatctgga cgcccattac attactcgcc tgttccagct
     6601 catcattgaa gattccgtat taactcagca ggctttgctc cagcaacatc tcaataaaat
     6661 taatccgcac tcagcacgca tcgcttttct cggccccaaa ggttcttatt cccatcttgc
     6721 ggcgcgccag tatgctgccc gtcactttga gcaattcatt gaaagtggct gcgccaaatt
     6781 tgccgatatt tttaatcagg tggaaaccgg ccaggccgac tatgccgtcg taccgattga
     6841 aaataccagc tccggtgcca taaacgacgt ttacgatctg ctgcaacata ccagtttgtc
     6901 gattgttggc gagatgacgt taactatcga ccattgtttg ttggtctccg gcactactga
     6961 tttatccgcc atcaatacgg tctacagcca tccgcagcca ttccagcaat gcagcaaatt
     7021 ccttaatcgt tatccgcact ggaagattga atataccgaa agtacgtctg cggcaatgga
     7081 aaaggttgca caggcaaaat caccgcatgt tgctgcgttg ggaagcgaag ctggcggcac
     7141 tttgtacggt ttgcaggtac tggagcgtat tgaagcgaat cagcgacaaa acttcacccg
     7201 atttgtggtg ttggcacgta aagccattaa cgtttctgac caggttccgg cgaaaacgac
     7261 gttgttaatg gcgaccggac aacaagctgg tgcactggtt gaagcgttgc tggtactgcg
     7321 caatcacagt ctgattatga cccgtctgga atcacgtccg attcacggta atccgtggga
     7381 agagatgttt tatctggata ttcaggccaa tcttgaatca gcggaaatgc aaaaagcatt
     7441 gaaagagtta ggggaaatca cccgttcgat gaaggtattg ggctgttacc caagtgagaa
     7501 cgtagtgcct gttgatccaa cctgatgaaa aagtgccgga tgatgtgaat catccggcac
     7561 tggattatta ctggcgattg tcattcgcct gacgcaataa cacgcggctt tcactctgaa
     7621 aacgctgtgc gtaatcgccg aaccagtgct ccaccttgcg gaaactgtca ataaacgcct
     7681 gcttatcgcc ctgctccagc agctcaatcg cctcgccgaa acgcttatag taacgtttga
     7741 ttaacgccag attacgctct gacgacataa taatgtcggc ataaagctgc ggatcctgag
     7801 caaacagtcg cccgaccatc gccaactcaa ggcggtaaat cggcgaagag agcgccagta
     7861 gttgctcaag ctgaacattt tcttctgcca ggtgcagccc ataagcaaaa gtagcaaagt
     7921 ggcgcagagc ctgaataaac gccatattct gatcgtgctc gacagcgcta atacgatgca
     7981 accgagcgcc ccagacctga atttgctcca gaaaccattg gtatgcttcc ggcttacgtc
     8041 catcacacca gaccacaact tgctttgcca ggctaccgct gtccgggccg aacatcgggt
     8101 gtaaccccag caccgggcca tcgtgcgccg ccagcatggc ctgtaatggt ccatttttca
     8161 ccgatgccag atcaaccaga atacaatctt tcggtaaagg cggtaatttg ccgataactt
     8221 gctcagtaac gtggatgggc acactaacaa tcaccattcc ggcatcggca acaatatcag
     8281 ccgctcgatc ccagtcatgt tgctccagaa tccgcacctg ataacccgag agtgtcagca
     8341 tcttctcgaa cagacgtccc atctgaccgc caccaccgac gataaccacc gggcgcagtg
     8401 acggacaaag cgttttaaat cctttgtcgt tttcactgga gtaagattca cgcatcaccc
     8461 gacgcaaaac atcctcaatc agatctggcg gtacacccag agcttccgcc tctgcacgac
     8521 gcgaggccaa catagatgcc tcgcgctccg gaacataaat aggcagtcca aagcggcttt
     8581 tcacctcgcc cacttcagca accagttcca gacgcttcgc taataaattc agcagcgctt
     8641 tatcgacttc atcaatttga tcgcgtaatg cggtcaactc agcaaccata ataaacctct
     8701 taagccacgc gagccgtcag ctgcccgttc agatcctgat gaatttcacg cagcaaggca
     8761 tcggtcattt cccagctaat gcaggcatcg gttacggata caccgtattt catttcactg
     8821 cgcggttgct cggaagactg attgccctcg tggatattac tttcgatcat cagaccaata
     8881 attgagcgat tcccatcttt gatttgagca accacggatt ctgccaccgc aggctgacgg
     8941 cgataatctt tattggaatt accgtggctg caatctacca tcagagacgg gcgcagtccc
     9001 gcctgttcca tctctttttc acattgcgca acatccgcag ggctatagtt tggcgcttta
     9061 ccaccgcgca ggatcacatg cccgtccgga ttcccctgag tttgtagcaa cgcaacctgc
     9121 cctgcctggt taatgccaac aaaacggtgc ggctgggcgg cggcacgcat agcgttaatt
     9181 gccgttgcca gactgccatc ggtgccgttt ttaaaaccaa ccggcatgga aagcccggag
     9241 gccatttcac ggtgagtttg cgattccgtt gtacgagcac caattgctga ccagctaaac
     9301 agatcgccca ggtattgcgg gctattcgga tctaacgctt ccgtcgccag tggcagtccc
     9361 atattcacca gctcaagcag caatttacgc gcgatctgca gcccggcttc tacatcaaaa
     9421 gagccatcca tatggggatc gttaattaac cctttccagc cgacagtggt acggggtttt
     9481 tcaaaataga cgcgcattac cagatagagg ctatcgctga cctctgcggc aagggcttta
     9541 aatcgacgag catattccag agcagtttcc ggatcatgaa tggaacaagg accacatact
     9601 accagcagac gaggatcgcg cccggcgata atatctgaaa tggttttacg cgagtcagca
     9661 atctgggctt cttgttgcag gcttaatgga aaagcggcct tcagttgttc cggagtcatt
     9721 aaaacctgtt cgtcggtaat atgtacgtta ttcagcgcgt ctttctgcat gatggcgatc
     9781 ctgtttatgc tcgtttgcga tagttgatcc tcagcgagga tgacgtaacg ataacacata
     9841 aagtaaagtt ttcaatccat atttcgtaca c
//
LOCUS       DADSYM010000159         9853 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2415
            SAMN08039579-rid11723173.denovo.161, whole genome shotgun sequence.
ACCESSION   DADSYM010000159 DADSYM010000000
VERSION     DADSYM010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039579
            Sequence Read Archive: SRR6362533
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9853)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9853)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980162.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 03:39:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,484
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 4,347
            CDSs (with protein)               :: 4,347
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 43 of 85
            Pseudo Genes (incomplete)         :: 37 of 85
            Pseudo Genes (internal stop)      :: 19 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9853
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039579-rid11723173.denovo.161"
                     /strain="LGE2415"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8302 N 95.9628 W"
                     /collection_date="2015-08-24"
     gene            207..1301
                     /locus_tag="J1Y83_003729"
     CDS             207..1301
                     /locus_tag="J1Y83_003729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase A"
                     /protein_id="HBA7631839.1"
                     /translation="MEKLRVGIVFGGKSAEHEVSLQSAKNIVDAIDKSRFDVVLLGID
                     KQGQWHVSDASNYLLNADDPAHIALRPSATSLAQVPGKHEHQLIDAQNGQPLPTVDVI
                     FPIVHGTLGEDGSLQGMLRVANLPFVGSDVLASAACMDKDVTKRLLRDAGLNIAPFIT
                     LTRANRHNISFAEVESKLGLPLFVKPANQGSSVGVSKVTSEEQYTIAVDLAFEFDHKV
                     IVEQGIKGREIECAVLGNDNPQASTCGEIVLTSDFYAYDTKYIDEDGAKVVVPAAIAP
                     EINDKIRAIAVQAYQTLGCAGMARVDVFLTPENEVVINEINTLPGFTNISMYPKLWQA
                     SGLGYTDLITRLIELALERHAADNALKTTM"
     gene            complement(1325..1537)
                     /locus_tag="J1Y83_003730"
     CDS             complement(1325..1537)
                     /locus_tag="J1Y83_003730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414914.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2754 domain-containing protein"
                     /protein_id="HBA7631840.1"
                     /translation="MNLPVKIRRDWHYYAFAIGLIFILNGVVGLLGFEAKGWQTYAVG
                     LVTWVISFWLAGLIIRRRDEETENAQ"
     gene            1797..2105
                     /locus_tag="J1Y83_003731"
     CDS             1797..2105
                     /locus_tag="J1Y83_003731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2755 family protein"
                     /protein_id="HBA7631841.1"
                     /translation="MADFTLSKSLFSGKYRNASSTPGNIAYALFVLFCFWAGAQLLNL
                     LVHAPGVYERLMQVQETGRPRVEIGLGVGTIFGLIPFLVGCLIFAVVALWLHWRHRRQ
                     "
     gene            complement(2164..3258)
                     /gene="yaiW"
                     /locus_tag="J1Y83_003732"
     CDS             complement(2164..3258)
                     /gene="yaiW"
                     /locus_tag="J1Y83_003732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="surface-exposed outer membrane lipoprotein YaiW"
                     /protein_id="HBA7631842.1"
                     /translation="MSRVNPLSSLSLLAVLVLAGCSSQAPQPLKKGEKAIDVASVVRQ
                     KMPASVKDRDAWAKDLATTFESQGLAPTLENVCSVLAVAQQESNYQADPAVPGLSKIA
                     WQEIDRRAERMHIPAFLVHTALKIKSPNGKSYSERLDSVRTEKQLSAIFDDLISMVPM
                     GQTLFGSLNPVRTGGPMQVSIAFAEQHTKGYPWKMDGTVRQEVFSRRGGLWFGTYHLL
                     NYPASYSAPIYRFADFNAGWYASRNAAFQNAVSKASGVKLALDGDLIRYDSKEPGKTE
                     LATRKLAGKLGMSDSEIRRQLEKGDSFSFEETALYKKVYQLAEAKTGKSLPREMLPGI
                     QLESPKITRNLTTAWFAKRVDERRARCMKQ"
     gene            complement(3271..4491)
                     /gene="sbmA"
                     /locus_tag="J1Y83_003733"
     CDS             complement(3271..4491)
                     /gene="sbmA"
                     /locus_tag="J1Y83_003733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide antibiotic transporter SbmA"
                     /protein_id="HBA7631843.1"
                     /translation="MFKSFFPKPGTFFLSAFVWALIAVIFWQAGGGDWVARITGASGQ
                     IPISAARFWSLDFLIFYAYYIVCVGLFALFWFIYSPHRWQYWSILGTALIIFVTWFLV
                     EVGVAVNAWYAPFYDLIQTALSSPHKVTIEQFYREVGVFLGIALIAVVISVLNNFFVS
                     HYVFRWRTAMNEYYMANWQQLRHIEGAAQRVQEDTMRFASTLENMGVSFINAIMTLIA
                     FLPVLVTLSAHVPELPIVGHIPYGLVIAAIVWSLMGTGLLAVVGIKLPGLEFKNQRVE
                     AAYRKELVYGEDDATRATPPTVRELFSAVRKNYFRLYFHYMYFNIARILYLQVDNVFG
                     LFLLFPSIVAGTITLGLMTQITNVFGQVRGAFQYLINSWTTLVELMSIYKRLRSFEHE
                     LDGDKIQEVTHTLS"
     gene            4843..6000
                     /gene="ampH"
                     /locus_tag="J1Y83_003734"
     CDS             4843..6000
                     /gene="ampH"
                     /locus_tag="J1Y83_003734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine-
                     carboxypeptidase/endopeptidase AmpH"
                     /protein_id="HBA7631844.1"
                     /translation="MKRSLLFSAVLCAASLTSVHAAQPITEPEFASDIVDRYADHIFY
                     GSGATGMALVVIDGNQRVFRSYGETRPGNNVRPQLDSVIRIASLTKLMTSEMLVKLLD
                     QGTVKLNDPLSKYAPPGARVPTYNGTPITLVNLATHTSALPREQPGGAAHRPVFVWPT
                     REQRWKYLSTAKLKAAPGSQAAYSNLAFDLLADALANASGKPYTQLFEEQITRPLGMK
                     DTTYTPSPDQCRRLMVAERGASPCNNTLAAIGSGGVYSTPGDMMRWMQQYLSSDFYQR
                     SNQADRMQTLIYQRAQFTKVIGMDVPGKADALGLGWVYMAPKEGRPGIIQKTGGGGGF
                     ITYMAMIPQKNIGAFVVVTRSPLTRFKNMSDGINDLVTELSGNKPLVIPAS"
     gene            complement(6001..6624)
                     /gene="iprA"
                     /locus_tag="J1Y83_003735"
     CDS             complement(6001..6624)
                     /gene="iprA"
                     /locus_tag="J1Y83_003735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogen peroxide resistance inhibitor IprA"
                     /protein_id="HBA7631845.1"
                     /translation="MLSVVKPLQEFGKLDKCLSRNGTRFEFNNEKQVIFSSDVNSEDT
                     FVILEGVISLRREENVLIGITQAPYIMGLADGLMKNDIPYKLISEGNCTGYHLPAKQT
                     ITLIEQNQLWRDAFYWLAWQNRILELRDVQLIGHNSYEQIRATLLSMIDWNEELRSRI
                     GVMNYIHQRTRISRSVVAEVLAALRKGGYIEMNKGKLVAINRLPSEY"
     gene            complement(6710..9658)
                     /gene="ehaB"
                     /locus_tag="J1Y83_003736"
     CDS             complement(6710..9658)
                     /gene="ehaB"
                     /locus_tag="J1Y83_003736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter adhesin EhaB"
                     /protein_id="HBA7631846.1"
                     /translation="MHSWKKKLVVSQLALACTLAITSQANAATNDISGQTYNTFHHYN
                     DATYADDVYYDGYVGWNNYAADSYYNGDIYPVINNATVNGVISTYYLDDGISTNTNAN
                     SLTIKNSTIHGMIYSECMTTDCADRADDYYHDRLALTVDNSTIDDNYEHYTYNGTYNN
                     AADTHVVNVFNIGTAITLDQEVDLSISNNSHVAGITLTQGYEWEDIDDNTVSTGVNSS
                     EVFNNTITVKDSTVTSGSWTDEGTTGWFGNTGNASDYNGDGWNADDVALAVIAHPYAD
                     NAMQTTATFDNSTLMGDVFFSSNFDENFFPQGRDSYRDADGDVDTNGWDGTDRLDLTL
                     NNGSKWVGAAMSAHQIGVDTDDDGVNDSYTYGINTEATATLIDIAANSLWPSSTVGVE
                     NSDSEYSEFDHIIGNEVYQSGLFNVTLNTGSQWDTTKTSLIDTLSINSGSTVNVADST
                     LISDSISLTGLSALNINEDGHVATDSLTVDNSTVTISDEVSAGWAVGDAALYANNIKV
                     TNDGILDVGNTAANALQVDTLNLTSTTDTSGNIHAGVFNIESNRFVLDADLTNDRTND
                     TTKSNYGYGLIAMNSDGHLTINGNGDNDNTASIEAGQNEVDNNGDHVAAATGNYKVRI
                     DNATGAGSIADYNGNELIYVNDKNSNATFSAANKADLGAYTYQAEQRGNTVVLQQMEL
                     TDYANMTLSIPSANTNIWNLEQDTVGTRLTNSRHGLADNGGAWVSYFGGNFNGDNGTI
                     NYDQDVNGIMVGVDTKIDGNNAKWIVGAAAGFAKGDMNDRSGQVDQDSQTAYIYSSAH
                     FANNVFVDGSLSYSHFNNDLSATMSNGTYVDGSTNSDAWGFGLKAGYDFKLGDAGYVT
                     PYGSVSGLFQSGDDYQLSNDMKVDGQSYDSMRYELGVDAGYTFTYSEDQALTPYFKLA
                     YVYDDSNNDNDVNGDSIDNGTEGSAVRVGLGTQFSFTKNFSAYTDANYLGGGDVDQDW
                     SANVGVKYTW"
BASE COUNT         2541 a         2445 c         2341 g         2526 t
ORIGIN      
        1 acatggtcat ttaagccagg aataaattag cttcccgttc atcatttttg tgatcttgca
       61 cacatctttg agataacgca ctttcttcac cagtacgttg ctcgctttgt cagcagaaga
      121 ttgcaacatt ctgtcagaag gccgcgaatc ccccgttttg tcgtggtaat gtatgcactt
      181 tgtgttagac gggataggtt tttaagatgg aaaaactgcg ggtaggaatc gtttttggtg
      241 gtaaatcagc ggaacatgaa gtgtctctgc aatcggcaaa aaacattgtc gatgccattg
      301 ataaaagtcg cttcgatgtt gtgctgctgg gcattgataa acaagggcaa tggcacgtca
      361 gcgatgccag caattatctg ctaaatgcag acgatcctgc ccatattgcg ttgcgccctt
      421 cggcgaccag ccttgcgcag gtgccaggta aacatgagca tcagcttatc gacgcacaaa
      481 acggtcagcc gttgccgacg gtggatgtca ttttccctat tgtccacggt acgctgggcg
      541 aagatggttc cttgcaggga atgctgcggg tcgccaattt accgtttgta ggttctgatg
      601 ttctggcttc agcggcctgt atggataaag atgtcaccaa acgtctgctg cgcgatgccg
      661 ggctgaacat tgcgccattt attaccctga cgcgcgctaa tcgtcacaac atcagttttg
      721 ccgaagtgga gtctaaactg gggttaccgc tgtttgtaaa accggctaat cagggctctt
      781 ctgttggtgt cagcaaagta accagtgaag aacagtacac aattgccgtc gatctggcgt
      841 tcgagttcga tcataaagtg atcgttgagc aagggatcaa aggtcgtgaa atcgaatgcg
      901 cagttcttgg caacgataac ccgcaagcaa gcacctgtgg cgagatcgta ctcaccagcg
      961 atttctatgc ctacgacacc aagtacattg acgaagatgg cgcgaaagtg gtagttccgg
     1021 cagccattgc gccagaaatc aacgataaga tccgggcgat tgccgttcag gcttatcaga
     1081 cgttgggatg cgcaggcatg gcgcgtgtag acgtgttttt aaccccagag aacgaagtgg
     1141 tgatcaacga gatcaacacc ctgcctggct tcaccaacat cagtatgtat ccgaagctgt
     1201 ggcaagccag cggtctgggt tacaccgacc tgatcacgcg tttgattgag ctggcgctgg
     1261 agcgtcacgc tgcggataac gcactgaaaa ccacaatgta atattcaaac gcccggtaat
     1321 attcttattg ggcgttttca gtttcttcat cgcgacgacg aataatcaac cccgccagcc
     1381 agaaactaat cacccatgtc accagaccga cggcataggt ctgccaacct tttgcttcaa
     1441 atcccagtaa ccccaccacg ccattaagaa tgaatataag gccaatggcg aacgcatagt
     1501 agtgccagtc acggcggatt tttacaggca gattcatggc agctccgtta acgcaaaaca
     1561 acatccttgc atattctgta acaactgtct gtggtgtaga caacactttc gcaattgtta
     1621 ataaatttaa cttaaggttg ctattttgtg actcacaacg aagattcacg cttcgggaat
     1681 attcccaaca tcaatttacc atgattctga tattgacaat ctaaatgacc tgtcagactt
     1741 gccataagcc gctggtatca caccagaaga gaagcagatg acttccggag gtttttatgg
     1801 ctgatttcac cctgtcaaaa tcgctgttta gcggaaaata tcgcaatgcc tcttcaacgc
     1861 ctggcaatat tgcctatgcg ttgtttgtgc tgttttgctt ttgggctggg gcacaattgc
     1921 tgaacctgct agtgcatgcg cccggcgtct atgagcgttt aatgcaggtc caggaaacag
     1981 gtcgcccacg agtggaaatt ggtttaggtg tcggcaccat ttttgggctg atcccgtttt
     2041 tagtaggctg cctcattttt gcagtagtgg cgctatggct gcactggcga catcgccgcc
     2101 agtaagattt acgttgccag atgcggccta aaacgcctta tctggcgtaa cattcctgtt
     2161 tgatcactgt ttcatacaac gcgcccgccg ttcgtctacg cgcttcgcaa accaggccgt
     2221 agtcaggttg cgggtgattt tcgggctttc cagttgaatg ccaggcaaca tttcgcgggg
     2281 tagtgattta cccgttttcg cttcggcaag ttgataaact ttcttgtaca gcgccgtctc
     2341 ctcaaaagag aagctgtccc ctttctctaa ctggcggcga atttcactgt cgctcatccc
     2401 cagttttccc gccagtttgc gcgttgccag ttcggttttc ccgggctctt tactgtcgta
     2461 acgaattaaa tcaccgtcca gtgccagctt cacgccgctg gccttactga ccgcgttctg
     2521 aaacgcggca ttacggctgg cgtaccagcc agcgttaaaa tcagcaaaac ggtatatcgg
     2581 cgcgctatag ctggcgggat aattcagtaa atggtaagta ccaaaccaca acccgccgcg
     2641 acgactgaag acttcctgac ggactgtacc gtccattttc cacggatacc ctttggtatg
     2701 ctgttcggca aaagcaatgc tgacctgcat cggcccacct gtgcgcaccg gattgagcga
     2761 accaaacagc gtctgcccca ttggcaccat gctgatcaga tcgtcaaaaa tcgcgctcaa
     2821 ttgcttttct gtacgcaccg aatctaaccg ttcgctatag cttttgccgt ttggcgattt
     2881 gatttttagc gcggtatgca ccagaaaagc aggaatgtgc atccgttcag cacgacggtc
     2941 aatctcttgc catgcgatct tgcttaagcc cggaacggcc ggatctgcct gataatttga
     3001 ctcctgctgc gccaccgcca gcaccgagca gacgttttcc agcgtcggtg ccagcccctg
     3061 actttcaaag gtggtggcga gatctttcgc ccaggcatcg cggtctttca cgctcgcagg
     3121 catcttctgc cgcacgacgc tcgccacatc tatcgctttt tcgccctttt tcaacggttg
     3181 cggagcctga ctactacacc ccgctaaaac cagaaccgcc aacaatgaca gcgaagatag
     3241 aggattgacg cgcgacatta cttctccttt ttagctcaag gtatgggtta cttcctgaat
     3301 tttgtcacca tccagctcat gttcaaagct gcgcagacgt ttgtagatag acatcaactc
     3361 aaccagtgtg gtccatgagt taatcaggta ctggaaagca ccgcgaacct gaccaaaaac
     3421 gttagtaatc tgcgtcatca ggccgagtgt aatcgtaccg gcaacaattg acggaaacag
     3481 caagaacaaa ccgaaaacgt tatcgacctg caaatagaga atgcgggcga tattgaaata
     3541 catatagtga aaatagaggc ggaaatagtt tttccgtaca gcgctaaaaa gctcgcgtac
     3601 cgtaggcggc gtcgcgcgcg tggcatcgtc ttcaccataa accagctctt tacggtaggc
     3661 agcctctaca cgctggtttt taaactccag ccccggcagt ttgatcccta ccactgccag
     3721 taatccggtc cccatcagcg accagacgat agcggcaatc accagaccat acggaatgtg
     3781 cccgacaatc ggcagctccg gcacatgtgc ggagagcgtt accagcaccg gcaggaaggc
     3841 gatcaacgtc atgatagcgt tgataaagct gacgcccata ttctccagcg ttgaagcaaa
     3901 acgcatggtg tcttcctgca cacgctgtgc ggcaccttcg atatgacgca gttgttgcca
     3961 gttcgccatg taatattcgt tcatcgccgt acgccagcgg aacacgtagt gactgacaaa
     4021 gaagttgttc agcacactga tcaccaccgc gatcagcgca atccccagaa agacgcccac
     4081 ttcgcggtaa aattgttcga tggtgacttt atgcggcgaa cttagcgcgg tttgaatcag
     4141 atcatagaac ggcgcatacc aggcgttgac ggcgaccccg acttccacca aaaaccaggt
     4201 gacgaagatg atcagtgcag taccgagtat tgaccaatat tgccaacgat gcgggctata
     4261 gataaaccag aacaatgcaa aaagtcctac gcaaacaatg tagtaagcgt aaaaaatcag
     4321 gaaatccaac gaccagaaac gcgcggcgct aatcgggatc tgcccggaag ctccggtgat
     4381 acgcgccacc cagtcacccc cacctgcttg ccagaagata acggcaatca atgcccaaac
     4441 aaaggccgag agaaaaaacg ttcccggctt tgggaaaaaa gacttaaaca tatgcactcc
     4501 tgctaacttc ttatcgtttt aacaactgct gtgtattaac cgcatgaccg ctacaaagag
     4561 tcagccaaat gaaaccgccc attgtgaccc atagcaaggc gcttagttcg ccagcctgag
     4621 cataagaatt gtttcgacgg atttcacaaa ccaaagattg tttacataag cggattaaca
     4681 cgcgcaggat cacaaatgca ggcattggtg agcaggaaaa agatcgtgtt tacgcgccgt
     4741 tacgttttta acactattat tgcactaatt cgccctttgc aatccatcaa tgagtagtat
     4801 aaatacgctc agttaccttc attcaatcta tggacaccac cgttgaaacg tagtctgctt
     4861 ttttctgccg tgctgtgtgc ggcgtcattg acctccgtcc atgcggctca gccgatcact
     4921 gaaccggagt ttgcctctga tattgtcgat cgttatgccg atcatatttt ttacggcagc
     4981 ggtgccacgg ggatggcgct ggttgttatc gacggtaacc agcgcgtctt tcgcagttat
     5041 ggcgaaacgc gacctggtaa taacgttcgc ccgcagctgg attccgtcat acgtattgct
     5101 tccctcacca agttaatgac cagtgagatg ctggtgaaat tgctcgacca ggggaccgtg
     5161 aagttaaacg atccgctaag taaatatgct ccgccaggcg cacgcgtacc gacctacaat
     5221 gggacgccca ttacgctggt gaatctggca acccatacca gcgccctgcc ccgtgaacag
     5281 cccggtggcg cggcacatcg tccggtattt gtctggccaa cgcgcgagca acgctggaaa
     5341 tacctttcca ctgccaaact aaaagccgcg ccgggcagcc aggccgcgta ctccaacctc
     5401 gcattcgatc tgctggcaga tgcactggcg aatgcgtccg gcaagcctta tacccagttg
     5461 tttgaagagc agattacccg tccgttgggg atgaaagaca ccacctacac cccctcaccg
     5521 gatcagtgcc gccgtttaat ggttgctgag cgcggtgcca gtccgtgcaa taacacgctg
     5581 gcggcaattg gcagcggcgg cgtctattcc acgccaggcg atatgatgcg ctggatgcag
     5641 cagtatttgt catctgattt ttatcagcgc agtaaccagg ctgatcgcat gcagacgttg
     5701 atttatcagc gcgcgcagtt tacaaaagtg attggcatgg atgtccccgg caaagcggat
     5761 gcgcttggtt taggctgggt atatatggcc ccgaaagagg gtcgtccggg gattattcag
     5821 aagacaggtg gtggtggcgg tttcattacc tatatggcga tgatcccgca gaagaatatt
     5881 ggcgcgtttg tggtggtcac tcgttcgccg ttgacgcgct ttaaaaatat gagcgatggc
     5941 atcaatgacc tggtgaccga gctaagcggg aataaaccgt tggttatccc cgcatcctga
     6001 ttaatactct gaaggcaaac ggttgatagc gaccagtttg cctttattca tttcgatata
     6061 gccgccttta cgcaaagcag cgagaacttc cgcaacgaca gaacgcgata tacgtgtacg
     6121 ttgatggata taattcatca cgccaatacg tgatcgcaat tcttcattcc agtcaatcat
     6181 tgataataac gtcgcgcgga tttgttcgta ggaattatgc ccaatgagct gaacgtcgcg
     6241 taattccaga attctatttt gccaggctaa ccagtaaaaa gcgtctcgcc agagttgatt
     6301 ttgttcaata agagtaatgg tttgtttggc tggcaaatga tatcccgtac agtttccttc
     6361 tgatattaat ttgtatggta tatcgttttt cattaaacca tcagccagcc ccataatata
     6421 aggagcctgt gtaataccga taagtacgtt ttcttctcta cgcaaagaga taacgccctc
     6481 taaaataacg aaagtatctt cgctattgac atcactggaa aatataactt gcttttcatt
     6541 attaaactcg aagcgcgtac cgtttctgga caaacattta tcgagcttac caaattcctg
     6601 aagaggttta actacagata acatttgcgc gtcctttgca gtaatgcccg tcaaatcctt
     6661 gacgggcatt atttagatta aattacctat ttcttcggag tgaagaatat taccaggtat
     6721 atttaacacc cacattcgcg gaccagtctt gatctacgtc accaccaccg aggtagttag
     6781 catcggtata ggcgctgaag ttcttggtga agctgaactg agtacccaga ccaacacgta
     6841 ccgcagaccc ttcagtaccg ttatcgatgg aatcaccgtt cacatcgtta tcgttgttag
     6901 agtcgtcgta gacgtaagcc agtttgaagt acggagtcag agcctggtct tcgctgtagg
     6961 tgaaggtata acctgcatct acacccagtt cataacgcat gctgtcgtaa gactgaccat
     7021 caactttcat gtcgttgctc agctggtaat catcaccaga ctggaacaga ccagaaacgc
     7081 tgccgtaagg agtcacgtaa cctgcatcac ccagtttgaa gtcgtaaccg gctttcaaac
     7141 cgaagcccca agcgtcggag ttggtgctgc cgtcaacgta agtaccgttg ctcatggttg
     7201 cagacaggtc gttgttgaag tgagagtagc tcaagctacc gtcaacaaag acgttgttcg
     7261 cgaagtgagc agaagagtag atgtaggcag tctggctgtc ttgatccacc tgaccagaac
     7321 ggtcattcat atcaccttta gcgaagcctg cagccgcacc gacgatccac ttagcgttgt
     7381 taccgtcaat tttggtatca acaccgacca tgatgccgtt aacatcctga tcatagttga
     7441 tggtgccgtt gtcgccgttg aagttaccac cgaagtagct tacccatgcg ccgccgttat
     7501 cagccagacc atgacgagag ttggtcagac gagtaccaac ggtgtcttgt tccaggttcc
     7561 agatattggt gttcgcagac gggatgctca gcgtcatgtt agcgtagtcg gtcagctcca
     7621 tctgttgcag aacaacggtg ttaccacgtt gctcagcctg ataggtgtat gcacccaggt
     7681 cagctttgtt agcagcagag aaggtcgcgt tgctgttttt gtcgttgacg tagatcagct
     7741 cgttgccgtt gtagtcagcg atagaaccag caccagtagc gttgtcgata cgaactttgt
     7801 agttaccggt tgcagctgca acatggtcac cgttgttatc aacttcgttc tgaccagctt
     7861 cgatagaagc agtgttgtcg ttatcgccgt taccgttaat ggtcaggtga ccatcagagt
     7921 tcattgcgat taagccataa ccgtagtttg acttggtagt atcgttggta cggtcgttgg
     7981 tcaggtctgc atcaagtacg aagcggttgc tttcgatgtt gaacacacct gcgtgaatgt
     8041 taccactggt atcagtagtg ctggtcaggt tcagagtatc aacctgcaga gcattcgccg
     8101 cagtgttacc tacatccagg ataccgtcgt tagtcacttt gatgttgtta gcatacagag
     8161 cagcatcacc tacagcccag ccagcagaaa cttcatcaga aatggttacg gtactgttgt
     8221 cgacagtcag tgaatcagtt gcaacatgac catcttcgtt gatgttcagc gcagaaagac
     8281 cagtcagaga gatagagtca gaaatcagcg ttgaatcagc aacattaaca gttgaaccac
     8341 tgttgatgct cagagtatca atcagagaag ttttggtggt atcccactgt gaaccggtgt
     8401 tcagggtcac attgaacaga ccgctctggt aaacttcgtt accgatgatg tgatcaaatt
     8461 cgctgtattc agagtcgctg ttttctacac caacagttga tgaaggccac aggctgttag
     8521 ctgcgatgtc gatcagagtt gcagtggctt cagtattgat tccatatgta taggagtcat
     8581 ttacaccatc gtcatcagta tcaacaccaa tctgatgcgc agacattgcc gcaccgaccc
     8641 acttgctgcc attgttcagg gtcagatcca ggcgatcagt accatcccaa ccgttagtat
     8701 ctacatcacc gtcagcatca cgatagctat cacgaccttg tgggaagaag ttctcatcaa
     8761 aattacttga gaagaataca tcgcccatca gtgtggaatt gtcgaatgtt gcagtggttt
     8821 gcatggcgtt atcagcatat ggatgtgcaa taacagctaa tgcaacatca tcagcattcc
     8881 acccatcacc attgtaatca ctagcattac cagtattacc aaaccaacca gtagtacctt
     8941 catcagtcca tgaaccagag gtcacggtag aatctttaac agtgatggtg ttattaaata
     9001 cttcgctgct gtttacacca gtgctgacag tgttgtcgtc gatatcttcc cactcataac
     9061 cctgagtcag cgtaatacct gctacatgag agttattaga gatggacaga tcaacttcct
     9121 gatccagagt gatggcagta ccaatgttaa atacatttac aacatgagtg tcagcggcat
     9181 tattgtaggt gccgttatat gtgtagtgtt cgtagttgtc atcgatcgtt gaattatcaa
     9241 cggtcagcgc cagacgatca tggtagtagt catccgcacg atcagcacaa tcagttgtca
     9301 tgcactcgga ataaatcatg ccgtgaatag tgctgttttt gattgtcagg ctattggcgt
     9361 tggtattagt agaaatacca tcgtccagat agtaggtaga aatcacgccg ttaacggtag
     9421 cgttattaat gaccggatag atatcgccgt tgtaataact atcagcggca tagttattcc
     9481 agcctacata accatcatag taaacgtcat cagcataggt ggcgtcgttg tagtgatgga
     9541 aagtattgta agtttgacca gaaatatcgt tggtcgctgc attagcctga gaggtgatag
     9601 ccagagtgca agccaatgct aattgtgata ctacaagttt ctttttccag gagtgcattt
     9661 gtcatccctc ctcagggacg taataagttg atccatcaat tatcaatgca tagaaaacat
     9721 attatgtttc gcgtttacga ttatgcggcg ttatagagga atggttcaat tattctttcc
     9781 cctaagtccg attgcggaca tatataaagc acgtgaacac acacaaagaa agaaataaca
     9841 aaaacacaat att
//
LOCUS       DADSYM010000160         9599 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2415
            SAMN08039579-rid11723173.denovo.162, whole genome shotgun sequence.
ACCESSION   DADSYM010000160 DADSYM010000000
VERSION     DADSYM010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039579
            Sequence Read Archive: SRR6362533
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9599)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9599)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980162.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 03:39:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,484
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 4,347
            CDSs (with protein)               :: 4,347
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 43 of 85
            Pseudo Genes (incomplete)         :: 37 of 85
            Pseudo Genes (internal stop)      :: 19 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9599
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039579-rid11723173.denovo.162"
                     /strain="LGE2415"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8302 N 95.9628 W"
                     /collection_date="2015-08-24"
     gene            205..1362
                     /gene="acrE"
                     /locus_tag="J1Y83_003737"
     CDS             205..1362
                     /gene="acrE"
                     /locus_tag="J1Y83_003737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter periplasmic
                     adaptor subunit AcrE"
                     /protein_id="HBA7631847.1"
                     /translation="MTKHARFFLLPSFILISAALIAGCNDKGEEKAHVGEPQVTVHIV
                     KTAPLEVKTELPGRTNAYRIAEVRPQVSGIVLNRNFTEGSDVQAGQSLYQIDPATYQA
                     SYDSAKGELAKSEAAAAIAHLTVKRYVPLVGTKYISQQEYDQAIADARQADATVIAAK
                     ATVESARINLAYTKVTAPISGRIGKSTVTEGALVTNGQTTELATVQQLDPIYVDVTQS
                     SNDFMRLKQSVEQGNLHKENATSNVELVMENGQTYPQKGTLQFSDVTVDESTGSITLR
                     AVFPNPQHTLLPGMFVRARIDEGVQPDAILIPQQGVSRTPRGDATVLIVNNKSQIEAR
                     PVVASQAIGDKWLISEGLKSGDQVIVSGLQKARPGEQVKATTDTPADTASK"
     gene            1374..4478
                     /gene="acrF"
                     /locus_tag="J1Y83_003738"
     CDS             1374..4478
                     /gene="acrF"
                     /locus_tag="J1Y83_003738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit AcrF"
                     /protein_id="HBA7631848.1"
                     /translation="MANFFIRRPIFAWVLAIILMMAGALAILQLPVAQYPTIAPPAVS
                     VSANYPGADAQTVQDTVTQVIEQNMNGIDNLMYMSSTSDSAGSVTITLTFQSGTDPDI
                     AQVQVQNKLQLATPLLPQEVQQQGISVEKSSSSYLMVAGFVSDNPGTTQDDISDYVAS
                     NVKDTLSRLNGVGDVQLFGAQYAMRIWLDADLLNKYKLTPVDVINQLKVQNDQIAAGQ
                     LGGTPALPGQQLNASIIAQTRLKNPEEFGKVTLRVNSDGSVVRLKDVARVELGGENYN
                     VIARINGKPAAGLGIKLATGANALDTAKAIKAKLAELQPFFPQGMKVLYPYDTTPFVQ
                     LSIQEVVKTLFEAIMLVFLVMYLFLQNMRATLIPTIAVPVVLLGTFAILAAFGYSINT
                     LTMFGMVLAIGLLVDDAIVVVENVERVMMEDKLPPREATEKSMSQIQGALVGIAMVLS
                     AVFIPMAFFGGSTGAIYRQFSITIVSAMALSVLVALILTPALCATLLKPVSAEHHENK
                     GGFFGWFNTTFDHSVNHYTNSVGKILGSTGRYLLIYALIVAGMVVLFLRLPSSFLPEE
                     DQGVFLTMIQLPAGATQERTQKVLDQVTDYYLKNEKANVESVFTVNGFSFSGQAQNAG
                     MAFVSLKPWEERSGDENSAEAVIHRAKMELGKIRDGFVIPFNMPAIVELGTATGFDFE
                     LIDQAGLGHDALTQARNQLLGMAAQHPASLVSVRPNGLEDTAQFKLEVDQEKAQALGV
                     SLSDINQTISTALGGTYVNDFIDRGRVKKVYVQADAKFRMLPEDVDKLYVRSANGEMV
                     PFSAFTTSHWVYGSPRLERYNGLPSMEIQGEAAPGTSSGDAMALMENLASKLPAGIGY
                     DWTGMSYQERLSGNQAPALVAISFVVVFLCLAALYESWSIPVSVMLVVPLGIVGVLLA
                     ATLFNQKNDVYFMVGLLTTIGLSAKNAILIVEFAKDLMEKEGKGVVEATLMAVRMRLR
                     PILMTSLAFILGVLPLAISNGAGSGAQNAVGIGVMGGMVSATLLAIFFVPVFFVVIRR
                     CFKG"
     gene            4731..4952
                     /locus_tag="J1Y83_003739"
     CDS             4731..4952
                     /locus_tag="J1Y83_003739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA7631849.1"
                     /translation="MKRLIPVTLLTALLAGCAHDSPCVPVYDDQGRLVHTNTCMKGTT
                     QDNWETAGAIAGGAAAVAGLTMGIIALSK"
     gene            5383..6408
                     /locus_tag="J1Y83_003740"
     CDS             5383..6408
                     /locus_tag="J1Y83_003740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HBA7631850.1"
                     /translation="MKKMMIATLAAASVLLAVANQAHAGATLDAVQKKGFVQCGISDG
                     LPGFSYADADGKFSGIDVDVCRGVAAAVFGDDTKVKYTPLTAKERFTALQSGEVDLLS
                     RNTTWTSSRDAGMGMAFTGVTYYDGIGFLTHDKAGLKSAKELDGATVCIQAGTDTELN
                     VADYFKANNMKYTPVTFDRSDESAKALESGRCDTLASDQSQLYALRIKLSNPAEWIVL
                     PEVISKEPLGPVVRRGDDEWFSIVRWTLFAMLNAEEMGINSQNVDEKAANPATPDMAH
                     LLGKEGDYGKDLKLDNKWAFNIIKQVGNYSEIFERNVGSESPLKIKRGQNNLWNNGGI
                     QYAPPVR"
     gene            6476..7657
                     /locus_tag="J1Y83_003741"
     CDS             6476..7657
                     /locus_tag="J1Y83_003741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HBA7631851.1"
                     /translation="MSHRRSTVKGSLSFANPTVRAWLFQILAVVAVVGIIGWLFHNTV
                     TNLSNRGITSGFAFLDRGAGFGIVQHLIDYQQGDTYGRVFIVGLLNTLLVSALCIVFA
                     SVLGFFIGLARLSDNWLLRKLSTIYIEIFRNIPPLLQIFFWYFAVLRNLPGPRQAVSA
                     FDLAFLSNRGLYIPSPQLGDGFIAFILAVVMAIVLSVGLFRFNKTYQIKTGQLRRTWP
                     FAAVLIIGLPLLAQWLFGAALLWDVPALRGFNFRGGMVLIPELAALTLALSVYTSAFI
                     AEIIRAGIQAVPYGQHEAARSLGLPNPVTLRQVIIPQALRVIIPPLTSQYLNIVKNSS
                     LAAAIGYPDMVSLFAGTVLNQTGQAIETIAMTMSVYLIISLTISLLMNIYNRRIAIVE
                     R"
     gene            7667..8770
                     /locus_tag="J1Y83_003742"
     CDS             7667..8770
                     /locus_tag="J1Y83_003742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709065.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HBA7631852.1"
                     /translation="MTKVLLSHPPRLASHNSSRAMVWVRKNLFSSWSNSLLTIGCIWL
                     MWELIPPLLNWVFLQANWVGSTRADCTKSGACWVFIHERFGQFMYGLYPHDQRWRINL
                     ALLIGLVSIAPMFWKILPHRSRYIAVWAVIYPLVVWWLMYGGFLGLDRVETRQWGGLT
                     LTLIIASVGIAGALPWGILLALGRRSHMPIVRILSVIFIEFWRGVPLITVLFMSSVML
                     PLFMAEGTSIDKLIRALVGVILFQSAYVAEVVRGGLQALPKGQYEAAESLALGYWKTQ
                     GLVILPQALKLVIPGLVNTIIALFKDTSLVIIIGLFDLFSSVQQATVDPAWLGMSTEG
                     YVFAALIYWIFCFSMSRYSQHLEKRFNTGRTPH"
     gene            8778..9536
                     /locus_tag="J1Y83_003743"
     CDS             8778..9536
                     /locus_tag="J1Y83_003743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HBA7631853.1"
                     /translation="MSQILLQPANAMITLENVNKWYGQFHVLKNINLIVQPGERIVLC
                     GPSGSGKSTTIRCINHLEEHQQGRIVVDGIELNEDIRNIERVRQEVGMVFQHFNLFPH
                     LTVLQNCTLAPIWVRKMPKKEAEALAMHYLERVRIAEHAQKFPGQISGGQQQRVAIAR
                     SLCMKPKIMLFDEPTSALDPEMVKEVLDTMIGLAQSGMTMLCVTHEMGFARTVADRVI
                     FMDRGEIVEQAAPDEFFAHPKSERTRAFLSQVIH"
BASE COUNT         2290 a         2277 c         2465 g         2567 t
ORIGIN      
        1 acttctttaa atccgcgtca aaataaaaca ataggatatt aatctttttt tgtgtttatg
       61 tgccttgagt cgccagtatt cataactatt ccttacatcg acgaatgata atttgtagga
      121 tagcgaactg tatttttctt tctgcgagtt aacgcattgc ctttttgggc aaataacgcg
      181 cttttggttt tttgaggaat agtaatgacg aaacatgcca ggtttttcct cctgccctcc
      241 tttattctga tctccgcggc tttaatcgcc ggttgtaacg ataagggaga agagaaagct
      301 cacgtcggtg aaccgcaggt taccgttcat attgtgaaaa cggccccgtt agaagttaag
      361 actgaattac caggccgcac caatgcttac cgtatagccg aagttcgccc acaggttagc
      421 gggatcgtac tgaatcgcaa tttcactgaa ggcagcgatg tgcaagcagg ccagtccctg
      481 taccagatcg atcccgcgac ctatcaggca agttatgaca gcgcgaaagg cgaactagcg
      541 aaaagtgaag ccgccgccgc catcgcgcat ttgacggtaa aacgttacgt tccgctcgtg
      601 ggtacgaaat acatcagtca gcaggagtac gaccaggcca ttgctgatgc tcgccaggcc
      661 gatgccaccg tgattgccgc aaaagccaca gttgaaagcg ctcgcatcaa tctcgcttat
      721 accaaagtga ctgcgcctat tagcggacgt atcggcaaat cgactgtgac cgaaggcgct
      781 cttgtcacta atgggcaaac gactgaactg gcgactgtcc agcagctcga tcctatctac
      841 gttgatgtga cccaatccag caacgatttt atgaggctga agcaatccgt agagcaagga
      901 aatttgcata aggaaaacgc caccagcaac gtagagttgg tcatggagaa cggtcaaacc
      961 tatccccaga aaggtacgct gcaattctcc gatgtgaccg ttgatgaaag caccggctcc
     1021 ataaccctac gtgctgtctt ccctaacccg caacatacgc ttttgccggg tatgtttgtg
     1081 cgcgcacgga ttgatgaagg cgtccaacct gacgccattc ttatcccgca acaaggcgtt
     1141 agccgcacac cacgtggtga tgcaaccgtg ctgattgtta acaataaaag tcagattgaa
     1201 gcgcgccctg tcgttgccag tcaggcgatt ggcgataaat ggttgattag cgaaggtctg
     1261 aaatctggcg atcaagtcat tgtcagcggc ctgcaaaaag cgcgtccggg agagcaggtt
     1321 aaagccacta ccgatacccc cgcagatact gcatcgaagt aaggcaatct gacatggcaa
     1381 acttttttat tcgacgaccg atatttgcat gggtgctggc cattattctg atgatggcgg
     1441 gcgcactggc gatcctacaa ttgcccgtcg cccagtatcc aaccattgcg ccgcctgcgg
     1501 tttctgtttc ggcaaactat ccgggtgctg atgcgcagac cgtgcaggat acagtgacgc
     1561 aggttatcga acagaatatg aacggtatcg ataacctgat gtatatgtcc tccaccagcg
     1621 attccgccgg tagcgtgaca attaccctta ccttccagtc cgggaccgat cctgatatcg
     1681 cgcaagtaca ggtgcagaac aaactccagc tcgccacgcc gttgctgccg caggaggttc
     1741 agcagcaggg gatcagtgtt gaaaagtcca gtagcagcta tttgatggtg gcgggctttg
     1801 tctctgataa ccctggcacc acacaggacg atatctcgga ctatgtggcc tctaacgtta
     1861 aagatacgct tagccgtctg aatggcgtcg gtgacgtaca gcttttcggc gcacagtatg
     1921 cgatgcgtat ctggctggac gccgatctgc taaacaaata taaactgaca ccggttgatg
     1981 tgattaacca gttgaaggta cagaacgatc agatcgctgc cggacagttg ggcggaacgc
     2041 cagcgttacc agggcaacag ttgaacgcct cgattattgc tcagacgcgg ttaaaaaatc
     2101 cggaagaatt cggcaaagtg accctgcgcg taaacagtga cggctcggtg gtgcgtctga
     2161 aagatgtcgc acgggttgaa cttggcggtg aaaactataa cgttatcgct cgcatcaacg
     2221 gaaaaccggc ggcgggcctg gggattaagc tggcaaccgg cgcgaatgct ctcgataccg
     2281 cgaaagccat taaggcaaaa ctggcggaat tacagccatt cttcccgcag ggaatgaagg
     2341 ttctttaccc ttatgacacc acgcctttcg tccagctttc tatccaggaa gtggtgaaaa
     2401 cgctgttcga agccattatg ctggtattcc tggtgatgta tctgttcttg cagaacatgc
     2461 gggcaacatt gatacccacc attgctgtac ctgtcgtgtt gttagggaca tttgccatac
     2521 tcgccgcttt tggttactcc atcaatacgc taacgatgtt tgggatggta cttgccatcg
     2581 gacttcttgt tgatgatgcg atagtcgttg tggagaacgt cgagcgcgta atgatggagg
     2641 ataaactccc ccccagagaa gcgacggaaa aatcgatgtc gcaaatccag ggcgcgctgg
     2701 taggtatcgc gatggtactg tcggcggtat ttattccgat ggcattcttc ggtggctcaa
     2761 caggggcaat ttatcgacag ttctctatca ctattgtttc agcaatggcg ctttcagtac
     2821 tggttgcgct gattctcacc cccgcgttat gtgcaacatt gctgaaacca gtctctgccg
     2881 agcatcacga aaataaggga ggattcttcg gctggtttaa caccactttc gatcatagcg
     2941 ttaatcacta caccaatagc gttggcaaaa tcctcggttc cacagggcga tatttactga
     3001 tctatgcgct gattgttgca ggaatggtgg tgttgttttt acgtcttccg tcttccttct
     3061 tacctgaaga ggatcagggt gtctttctga ccatgattca gttacccgct ggcgcgacgc
     3121 aagagcggac gcaaaaagtg ttggatcaag tgacggatta ctatctgaag aacgagaaag
     3181 cgaacgttga aagtgtcttt acagttaacg gctttagctt cagcggccag gcacaaaacg
     3241 ccggtatggc cttcgtcagt ctgaaaccgt gggaagagcg tagtggtgac gaaaacagtg
     3301 cggaagcagt aatccatcgt gccaaaatgg aattgggcaa gatccgcgac ggttttgtca
     3361 ttccatttaa tatgccagcc attgttgaac taggcacggc aacgggtttc gactttgagt
     3421 taattgatca ggctgggctg ggtcacgatg ccctgaccca ggcccgtaac cagttgcttg
     3481 gcatggcggc gcaacatcct gccagcttag tcagcgtgcg ccctaatggc ctggaagaca
     3541 ccgcgcagtt taaactggaa gttgaccagg aaaaggcgca ggcattaggt gtttcacttt
     3601 ctgacatcaa tcagaccatt tcaacggcgt tgggtgggac ttacgttaac gacttcatcg
     3661 accgtggtcg cgtgaaaaag gtgtatgttc aggcggatgc caaattccgt atgctgccag
     3721 aagatgtcga taaactttat gtccgcagcg ccaacggcga aatggtgcca ttctctgcct
     3781 ttaccacttc acattgggtg tatggctctc cgcgactgga acgctacaac ggtctgccgt
     3841 caatggagat tcagggggaa gccgcgccag gaaccagttc cggcgatgct atggcgttga
     3901 tggaaaacct tgcgtcaaaa ttaccggcgg gcattggtta tgactggacg ggtatgtcat
     3961 atcaggaacg tttatcggga aaccaggctc ccgctctggt agcaatttcc tttgtggttg
     4021 ttttcctgtg ccttgctgca ctctatgaaa gctggtcaat tcctgtctcg gttatgttgg
     4081 tcgtgccgtt agggattgtc ggcgtgctac tggcggcaac actctttaat caaaaaaatg
     4141 acgtctactt tatggtgggc ttgctaacga caattggctt gtcggccaaa aacgctattt
     4201 tgattgttga gttcgctaaa gatctcatgg agaaagaggg taaaggtgtt gttgaagcga
     4261 cactaatggc agtacgtatg cgtctgcgtc ctatcctgat gacctctctc gcctttattc
     4321 tcggcgtatt accgctggct atcagtaacg gggccggcag tggcgcgcaa aacgcagtcg
     4381 gtatcggggt aatgggcgga atggtctctg caacgttgct ggcaatcttc ttcgtgccag
     4441 tgttctttgt ggtgatccgt cgatgcttta aaggataaat cagaaacata aaggcgcttt
     4501 caggcgcctt ttttattttc agtgaacccc ataaaaatta ataagatatt cttcttctca
     4561 ctctttaaaa actttctata gttcccgctc ccttcactat ttttacaatt cacataaatt
     4621 gtcattattc attccgcaag atttataacc tccagtgata aaatggcatt cagctcgtta
     4681 ataagagagt taacttatta agcgttagcg tttattactg aggtaacacc atgaaaagat
     4741 taattcctgt gacattgctc accgcattgc tggcgggctg tgctcacgat tcgccctgcg
     4801 ttccggttta tgacgatcag gggcgtctgg ttcacaccaa tacctgtatg aaaggcacga
     4861 ctcaggataa ctgggaaacg gctggggcta ttgccggcgg ggcagcagct gttgctggcc
     4921 tgacgatggg gatcatcgct ctgtcgaaat aactaaacag ggcgcggagc tccgcgctct
     4981 ttttaacgat acagtatctt tccataagat aaaatattgc tatattctgc tgggtaattc
     5041 cctgctgatt tagcatgtta tatcacgtta tttcgctgct ttcgaaataa cgcatcaatt
     5101 taactttcac acatcccacc agaaatatac tcacgctaat aatcattatt ctgcttagta
     5161 aaaaacgcgt aaatattttg cgccaaaatg tggcgcatgt ttcattttcg caccattgcg
     5221 gggcgctgtt tttattattt cctgtctaca ctcagcttat tgcgacgcga aatcgtgcgc
     5281 aaaagctggc actacttttg cttataagag gatggccaca gacaggtaaa agacgtttcc
     5341 caaacgtcct ataacgataa ttttcgcctc acaggaagca ttatgaaaaa gatgatgata
     5401 gccacactgg ctgccgccag cgtgctgctt gccgttgcaa atcaggcgca tgctggcgcg
     5461 acgcttgatg ccgttcagaa aaaaggtttt gtgcaatgcg ggatcagtga tggattacct
     5521 gggttctctt atgccgatgc tgacggtaag ttttcaggta ttgatgtaga tgtttgtcgt
     5581 ggtgttgccg ctgctgtatt cggtgacgat acgaaagtga aatatacccc gctcactgca
     5641 aaagaacgct tcaccgcttt acagtcaggg gaggtggatt tgctctcccg taatacgacc
     5701 tggacttcat ctcgcgatgc cgggatggga atggcattta ctggcgtcac ttattacgac
     5761 ggcattggct tcctgacgca cgataaagcg gggctaaaaa gcgcgaaaga actggatggc
     5821 gctaccgtct gtattcaggc ggggactgat accgaactca acgtcgccga ttacttcaag
     5881 gcaaataata tgaagtacac gccagtaacc ttcgatcgct cagacgaatc agcgaaagct
     5941 ctggaatctg gccgctgtga tactctggcc tcggatcaat cacaactgta tgccctacgc
     6001 atcaaattaa gcaacccagc tgaatggatt gtcttaccgg aagttatctc taaagaacca
     6061 cttgggccgg tagttcgtcg tggcgatgat gaatggttct cgattgtacg ctggacgctt
     6121 ttcgccatgc tgaatgctga agagatgggc atcaattccc agaacgtcga tgaaaaagcg
     6181 gctaatccag caacgcctga tatggcacat ctgctgggta aagaaggcga ttacggcaag
     6241 gatctgaagc tggataataa atgggccttt aacatcatca aacaggtggg taactactcg
     6301 gaaattttcg agcgtaacgt aggttcagaa agcccgctga aaattaaacg tgggcaaaat
     6361 aatctctgga ataacggcgg tattcagtac gcgccgcccg tgcgttaagt cggtgtgagg
     6421 taacgagcgt cgctacggcg gcgctctctt cagcgttatg ctttccgagg tttctatgtc
     6481 tcatcgccgc tcaaccgtta aaggctcact ctcttttgcc aaccctacgg ttcgcgcctg
     6541 gttattccag atccttgccg ttgttgctgt tgtcggcatt attggttggt tatttcacaa
     6601 cactgtaacg aatctcagta atcgcggcat aacatcagga tttgcttttc ttgaccgcgg
     6661 ggccggtttt ggcatcgttc agcatttgat tgattatcag caaggcgaca cttacggacg
     6721 cgtttttatt gtcggcctgc tcaatacgct actggtttct gcattgtgta ttgtgttcgc
     6781 ttctgttttg ggcttcttta tcggtctggc gagactttcg gataactggc tgctacgaaa
     6841 gctttccaca atttatattg agatcttccg taatattccg ccattgctgc aaatcttctt
     6901 ctggtacttt gccgtgttgc gcaatttgcc cggaccacga caggcagtga gcgcgtttga
     6961 tctcgccttt ttgagcaatc gtgggcttta tattccgtcg ccgcagctgg gagacggatt
     7021 tattgcgttt atcctcgctg ttgttatggc tatagtcctt tcagttgggc tattccgctt
     7081 taataaaaca taccagataa agaccggaca actgcgccgc acctggccat tcgccgctgt
     7141 gttgatcatt ggtttgcctt tactggcgca atggcttttt ggcgcagcac tactctggga
     7201 tgtcccagcc cttcgaggct ttaatttccg cggcgggatg gttttaattc ctgaactggc
     7261 agccttaacg ctggcgcttt cggtttatac ttctgcattt atcgccgaaa ttatccgcgc
     7321 agggatccag gcagtgcctt atggtcaaca tgaagcagct cggtcactgg gattacccaa
     7381 cccggtcacg ctacgccagg tcattattcc ccaggcattg cgggtgatta ttccaccgtt
     7441 aaccagccag tatctcaaca tcgtcaagaa ctcctctctt gccgccgcta ttggctatcc
     7501 cgatatggtt tcgctgtttg ctggcaccgt gctgaaccaa acggggcaag ccatcgagac
     7561 gatagccatg accatgtcgg tctatctgat tatcagcctg actatctcgc tgctgatgaa
     7621 tatctataac cgccgcatcg cgatcgttga acgctaagga gtcatgatga caaaagtatt
     7681 gctgtctcat cccccgcgcc tggcgagcca taactcaagc cgcgcgatgg tgtgggtgcg
     7741 aaaaaatctg ttctccagct ggagcaatag cctgctgact attggctgca tatggttgat
     7801 gtgggaactg atcccaccgt tgctgaactg ggtatttttg caggctaact gggttggctc
     7861 aacgcgtgcc gactgcacaa aatccggtgc ctgttgggtc ttcatccacg aacgatttgg
     7921 tcagtttatg tatggacttt acccacacga ccaacgctgg cgaattaacc tggcattact
     7981 gattggcctt gtttctatcg caccaatgtt ctggaaaata cttccgcatc gcagtcgcta
     8041 tattgcggtc tgggcggtga tttacccact ggttgtctgg tggctgatgt atggtgggtt
     8101 tcttggtctt gatcgggttg aaacccggca atggggcggg ttgacgctaa ctttaattat
     8161 tgcatcagtt gggattgcgg gggcgctacc gtgggggatc ttactggcgt taggtcgccg
     8221 ctcccatatg ccgattgtgc gtatcttatc ggtcattttt atcgagttct ggcgcggcgt
     8281 accgctgatt accgtattgt ttatgtcttc ggtcatgctg ccgttgttta tggcagaagg
     8341 caccagtatc gacaaactga tccgcgcgct ggttggcgtg atcctgtttc agtcagcata
     8401 tgttgcggaa gtcgtgcgag gcggattgca ggcgctgcct aaagggcaat atgaagcggc
     8461 agagtcgctg gcattgggtt actggaaaac tcaggggctg gttattctgc cacaggcgtt
     8521 gaagctggta attcctgggc tggtaaatac catcatcgca ctcttcaaag ataccagtct
     8581 ggtgatcatc atcgggttgt tcgatctttt cagtagcgtt cagcaggcaa ccgttgatcc
     8641 cgcctggttg ggtatgtcga cggaagggta tgttttcgcc gcgctgatct actggatctt
     8701 ctgtttcagc atgtcacgtt atagccaaca tctggaaaaa cgttttaaca ccgggcgtac
     8761 accgcattga ggacactatg agccaaattt tactgcaacc tgctaacgcg atgattacgc
     8821 tggaaaacgt caataaatgg tatggacaat tccatgtttt gaaaaatata aatttaatcg
     8881 tgcaaccggg cgaacggatc gttctgtgtg gcccttcagg ttccggtaaa tcgacaacca
     8941 ttcgttgtat taatcatctg gaagaacatc aacagggacg gatcgtggta gatggcatcg
     9001 aacttaatga agatatccgc aatattgagc gcgtcaggca ggaagtggga atggtctttc
     9061 agcatttcaa tctcttccct catctgaccg tgttgcagaa ctgtaccctg gcaccgattt
     9121 gggtacgcaa gatgccaaag aaagaggctg aagctctggc gatgcattac cttgagcggg
     9181 tgagaattgc cgaacatgcc cagaagtttc ccggacagat ttctggtggt cagcagcaac
     9241 gtgttgccat agcacgttcg ctgtgtatga agccgaaaat tatgttgttt gatgagccaa
     9301 cgtcggcgct cgatcctgag atggtgaaag aggtgctgga tacgatgatt gggctggcgc
     9361 agtcgggtat gacaatgttg tgtgtaacac atgagatggg gtttgcacga accgtcgctg
     9421 accgggtgat ttttatggat cgtggggaaa tagtggagca agctgcgcct gatgaatttt
     9481 ttgcgcatcc taaatcagag cgtacgaggg catttttatc gcaggtaatc cattaattga
     9541 atgttagttc gaaaagcaaa aaggccatcc tttcggatgg cctttcgctt gatttgatg
//
LOCUS       DADSYM010000161         9571 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2415
            SAMN08039579-rid11723173.denovo.163, whole genome shotgun sequence.
ACCESSION   DADSYM010000161 DADSYM010000000
VERSION     DADSYM010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039579
            Sequence Read Archive: SRR6362533
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9571)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9571)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980162.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 03:39:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,484
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 4,347
            CDSs (with protein)               :: 4,347
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 43 of 85
            Pseudo Genes (incomplete)         :: 37 of 85
            Pseudo Genes (internal stop)      :: 19 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9571
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039579-rid11723173.denovo.163"
                     /strain="LGE2415"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8302 N 95.9628 W"
                     /collection_date="2015-08-24"
     gene            31..984
                     /gene="ompT"
                     /locus_tag="J1Y83_003744"
     CDS             31..984
                     /gene="ompT"
                     /locus_tag="J1Y83_003744"
                     /EC_number="3.4.23.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="omptin family outer membrane protease OmpT"
                     /protein_id="HBA7631854.1"
                     /translation="MRAKLLGIVLTTPIAISSFASTETLSFTPDNINADISLGTLSGK
                     TKERVYLAEEGGRKVSQLDWKFNNAAIIKGAINWDLMPQISIGAAGWTTLGSRGGNMV
                     DRDWMDSSNPGTWTDESRHPDTQLNYANEFDLNIKGWLLNEPNYRLGLMAGYQESRYS
                     FTARGGSYIYSSEEGFRDDIGSFPNGERAIGYKQRFKMPYIGLTGSYRYEDFELGGTF
                     KYSGWVEASDNDEHYDPGKRITYRSKVKDQNYYSVAVNAGYYVTPNAKVYVEGSWNRV
                     TNKKGNTSLYDHNDNTSDYSKNGAGIENYNFITTAGLKYTF"
     gene            1216..1884
                     /locus_tag="J1Y83_003745"
     CDS             1216..1884
                     /locus_tag="J1Y83_003745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HBA7631855.1"
                     /translation="MVKLMKKNTDDGAKIYTPLTLKLYDWWVLGVSNRLAWGCPTKEH
                     LLPHFLEHLGNNHLDIGVGTGFYLTHVPESSLISLMDLNEASLNAASTRAGESKIKHK
                     ISHDVFDPYPTALHGQFDSISMFYLLHCLPGNISTKSCVIRNAAQALTDDGTLYGATI
                     LGDGVVHNSFGQKLMRIYNQKGIFSNTKDSEEGLTHILSEHFENVKTKVKGTVVMFSA
                     SGKK"
     gene            complement(1939..2243)
                     /locus_tag="J1Y83_003746"
                     /pseudo
     CDS             complement(1939..2243)
                     /locus_tag="J1Y83_003746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000259980.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
     gene            2301..2600
                     /locus_tag="J1Y83_003747"
     CDS             2301..2600
                     /locus_tag="J1Y83_003747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001707862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage integrase family protein"
                     /protein_id="HBA7631856.1"
                     /translation="MRWNGTRDLQKNQAKRLIDESPEPLKSVVKFALVTVLRKSNIIN
                     LEWQQIDMQRQVARVNPEDSKSNCAIGVALNDTASKVLRDQTGKHHKCGCTYQGG"
     gene            2620..2928
                     /locus_tag="J1Y83_003748"
     CDS             2620..2928
                     /locus_tag="J1Y83_003748"
                     /inference="COORDINATES: protein motif:HMM:NF012798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HBA7631857.1"
                     /translation="MPALRKMRIDSKTAWLSACRRAGIENFRFHDLRHTRASWLIPSG
                     VPLSVLQEMGGWESREMVRRYAHLAPNHLTEHARKIDDIFGDNVPLWNYRSNKEGVTD
                     "
     gene            complement(2936..3012)
                     /locus_tag="J1Y83_003749"
     tRNA            complement(2936..3012)
                     /locus_tag="J1Y83_003749"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2976..2978),aa:Arg,seq:tct)
     gene            3283..4149
                     /gene="folD"
                     /locus_tag="J1Y83_003750"
     CDS             3283..4149
                     /gene="folD"
                     /locus_tag="J1Y83_003750"
                     /EC_number="1.5.1.5"
                     /EC_number="3.5.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HBA7631858.1"
                     /translation="MAAKIIDGKTIAQQVRSEVAQKVQARIAAGLRAPGLAVVLVGSN
                     PASQIYVASKRKACEEVGFVSRSYDLPETTSEAELLELIDALNADNTIDGILVQLPLP
                     AGIDNVKVLERIHPDKDVDGFHPYNVGRLCQRAPRLRPCTPRGIVTLLERYNIDTFGL
                     NAVVIGASNIVGRPMSMELLLAGCTTTVTHRFTKNLRHHVENADLLIVAVGKPGFIPG
                     DWIKEGAIVIDVGINRLENGKVVGDVVFEDAAKRASYITPVPGGVGPMTVATLIENTL
                     QACVEYHDPQGE"
     gene            4151..4363
                     /gene="ybcJ"
                     /locus_tag="J1Y83_003751"
     CDS             4151..4363
                     /gene="ybcJ"
                     /locus_tag="J1Y83_003751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308617.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated protein YbcJ"
                     /protein_id="HBA7631859.1"
                     /translation="MATFSLGKHPHVELCDLLKLEGWSESGAQAKIAIAEGQVKVDGA
                     VETRKRCKIVAGQTVSFAGHSVQVVA"
     gene            4471..4992
                     /locus_tag="J1Y83_003752"
     CDS             4471..4992
                     /locus_tag="J1Y83_003752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HBA7631860.1"
                     /translation="MPTVITHAAVPLCIGLGLGSKAIPPRLLFAGIVLAMLPDADVLS
                     FKFGVAYGNVFGHRGFTHSLVFAFVVPLLCVLIGRRWFRAGLIRCWLFLTVSLLSHSL
                     LDSVTTGGKGVGWLWPWSDERFFAPWQVIKVAPFALSRYTTPYGHQVIISELMWVWLP
                     GMLLMGMLWWRRR"
     gene            complement(5028..6413)
                     /gene="cysS"
                     /locus_tag="J1Y83_003753"
     CDS             complement(5028..6413)
                     /gene="cysS"
                     /locus_tag="J1Y83_003753"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HBA7631861.1"
                     /translation="MLKIFNTLTRQKEEFKPIHAGEVGMYVCGITVYDLCHIGHGRTF
                     VAFDVVARYLRFLGYKLKYVRNITDIDDKIIKRANENGESFVALVDRMIAEMHKDFDA
                     LNILRPDMEPRATHHIAEIIELTEQLIAKGHAYVADNGDVMFDVPTDPTYGVLSRQDL
                     DQLQAGARVDVVDDKRNPMDFVLWKMSKEGEPSWPSPWGAGRPGWHIECSAMNCKQLG
                     NHFDIHGGGSDLMFPHHENEIAQSTCAHDGQYVNYWMHSGMVMVDREKMSKSLGNFFT
                     VRDVLKYYDAETVRYFLMSGHYRSQLNYSEENLKQARAALERLYTALRGTDKTVAPAG
                     GEAFEARFIEAMDDDFNTPEAYSVLFDMAREVNRLKAEDMAAANAMASHLRKLSAVLG
                     LLEQEPEAFLQSGAQADDSEVAEIEALIQQRLDARKAKDWAAADAARDRLNEMGIVLE
                     DGPQGTTWRRK"
     gene            6587..7081
                     /gene="ppiB"
                     /locus_tag="J1Y83_003754"
     CDS             6587..7081
                     /gene="ppiB"
                     /locus_tag="J1Y83_003754"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase B"
                     /protein_id="HBA7631862.1"
                     /translation="MVTFHTNHGDIVIKTFDDKAPETVKNFLDYCREGFYNNTIFHRV
                     INGFMIQGGGFEPGMKQKTTKDPIKNEANNGLKNTRGTLAMARTQAPHSATAQFFINV
                     VDNDFLNFSGESLQGWGYCVFAEVVEGMDVVDKIKGVATGRSGMHQDVPKEDVIIESV
                     TVSE"
     gene            7084..7806
                     /gene="lpxH"
                     /locus_tag="J1Y83_003755"
     CDS             7084..7806
                     /gene="lpxH"
                     /locus_tag="J1Y83_003755"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HBA7631863.1"
                     /translation="MATLFIADLHLCAEEPAITAGFLRFLAGEARKADALYILGDLFE
                     AWIGDDDPNPLHRQMAAAIKAVSDSGVPCYFIHGNRDFLLGKRFARESGMTLLPEEKV
                     LELYGRRVLIMHGDTLCTDDAGYQAFRAKVHKPWLQTLFLALPLFVRKRIAARMRANS
                     KEANSSKSLAIMDVNQNAVVSAMEKHQVQWLIHGHTHRPAVHELIANQQPAFRVVLGA
                     WHTEGSMVKVTADDVELIHFPF"
     gene            7924..8433
                     /gene="purE"
                     /locus_tag="J1Y83_003756"
     CDS             7924..8433
                     /gene="purE"
                     /locus_tag="J1Y83_003756"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HBA7631864.1"
                     /translation="MSSRNNPARVAIVMGSKSDWATMQFAAEIFEILNVPHHVEVVSA
                     HRTPDKLFSFAESAEENGYQVIIAGAGGAAHLPGMIAAKTLVPVLGVPVQSAALSGVD
                     SLYSIVQMPRGIPVGTLAIGKAGAANAALLAAQILATHDKELHQRLNEWRKAQTDEVL
                     ENPDPRGAA"
     gene            8430..9497
                     /gene="purK"
                     /locus_tag="J1Y83_003757"
     CDS             8430..9497
                     /gene="purK"
                     /locus_tag="J1Y83_003757"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HBA7631865.1"
                     /translation="MKQVCVLGNGQLGRMLRQAGEPLGIAVWPVGLDAEPAAVPFQQS
                     VITAEIERWPETALTRELARHPAFVNRDVFPIIADRLTQKQLFDKLHLPTAPWQLLAE
                     RSEWPAVFDRLGELAIVKRRTGGYDGRGQWRLRAAETEQLPAECYGECIVEQGINFSG
                     EVSLVGARGFDGSTVFYPLTHNLHQDGILRTSVAFPQANAQQQAQAEEMLSAIMQELG
                     YVGVMAMECFVTPQGLLINELAPRVHNSGHWTQNGASISQFELHLRAITDLPLPQPVV
                     NSPSVMINLIGSDVNYDWLKLPLVHLHWYDKEVRPGRKVGHLNLTDSDTSRLTATLEA
                     LIPLLPPEYASGVMWAQSKFS"
BASE COUNT         2370 a         2428 c         2456 g         2317 t
ORIGIN      
        1 atcattaaaa cgattgaatg gagacctttt atgcgggcga aacttctggg aatagtcctg
       61 acaaccccta ttgcgatcag ctcttttgct tctaccgaga ctttatcgtt tactcctgac
      121 aacataaatg cggacattag tcttggaact ctgagcggaa aaacaaaaga gcgtgtttat
      181 ctagccgaag aaggaggccg aaaagtcagt caacttgact ggaaattcaa taacgctgca
      241 attattaaag gtgcaattaa ttgggatttg atgccccaga tatctatcgg ggctgctggc
      301 tggacaactc tcggtagccg aggtggcaat atggtcgatc gggactggat ggattccagt
      361 aaccccggaa cctggacgga tgaaagtaga caccctgata cacaactcaa ttatgccaac
      421 gaatttgatc tgaatatcaa aggctggctc ctcaacgaac ccaattaccg cctgggactc
      481 atggccggat atcaggaaag ccgttatagc tttacagcca gagggggttc ctatatctac
      541 agttctgagg agggattcag agatgatatc ggctccttcc cgaatggaga aagagcaatc
      601 ggctacaaac aacgttttaa aatgccctac attggcttga ctggaagtta tcgttatgaa
      661 gattttgagc taggtggcac atttaaatac agcggctggg tggaagcatc tgataacgat
      721 gagcactatg acccaggaaa aagaatcact tatcgcagta aagtcaaaga ccaaaattac
      781 tattctgttg cagtcaatgc aggttattac gtaacaccta acgcaaaagt ttatgttgaa
      841 ggctcatgga atcgggttac gaataaaaaa ggtaatactt cactttatga tcacaatgat
      901 aacacttctg actacagcaa aaatggtgca ggcatagaaa actataactt catcactact
      961 gctggtctta agtacacctt ttaacaacgt taaatagatt ttctccccgg tggtattgta
     1021 tagccccggg gaatttgttt accgttaaaa gcaaacaata tcccctaatc ttgtaagcat
     1081 ttaacctgcg tcctggcgtg taacctctta ccgcaacaca agactcaata tattcaaatt
     1141 cgttatgccg tggcatcatt taaccccttg taattcatcg ctataattga tttaattcac
     1201 aaataaaact ataacatggt gaaactaatg aaaaaaaaca cagatgatgg ggctaaaatt
     1261 tacacaccac ttaccctaaa gctttatgac tggtgggttt tgggtgtatc aaatcggctt
     1321 gcatggggat gtcctacaaa ggaacacctt cttccacact ttctggaaca tttaggtaac
     1381 aaccatctgg atattggcgt tggaactggg ttttacctta ctcacgtacc tgagagtagt
     1441 ctgatatctt taatggattt gaacgaagct agcctgaacg cggcatcgac aagggctggg
     1501 gaatcaaaaa ttaaacataa aattagccat gatgtttttg atccttatcc cacggcgtta
     1561 catggtcaat ttgattccat ttccatgttt taccttcttc actgcctgcc tggaaatata
     1621 tctacaaaaa gctgtgtaat acgcaatgcg gcgcaggcct taactgacga tggaactcta
     1681 tacggagcca caattcttgg tgacggcgtt gtgcacaata gcttcggtca aaaactgatg
     1741 cgcatttaca atcagaaagg catcttttca aacacaaaag attccgaaga aggcttaaca
     1801 catatactct cagagcattt cgagaatgtt aaaaccaagg ttaaaggtac tgtagtaatg
     1861 ttttccgctt cagggaaaaa atagcatcca gccgcagcac gttcttgcgc acgacgtgct
     1921 gcggcataat cccaatgatt actctctgac agggttcaca ggccactcaa tatcaggggc
     1981 agttgatgta tcaactcggt tcagcaacac ccgatacatc ttccaggctt ccagcaacga
     2041 ggtttcgtcc tccatatcct ttatccatga tgtgccgttc cacttctgaa actcccctgc
     2101 cggggatacc aggtgacatt ttccggtaac ggaccgagtt cagaaataaa taatgcgtcc
     2161 cctgatgcca cgtcataaac cgttttcccc cgatggtctt caacgagatg ccacgatgcc
     2221 tcatcactgt tgaaaacaac cacgaagcca gccggaatat ctggcggtgc aatatcagta
     2281 ctgtttgcag gcaaacctgt atgaggtgga atggcacaag agacttacaa aagaatcagg
     2341 ccaaacgcct gattgatgag agccccgaac cactgaaatc tgtcgtcaaa tttgcgctgg
     2401 taactgttct gagaaagtcg aacatcataa atctggagtg gcaacagatt gacatgcagc
     2461 gacaagttgc cagggtgaat ccagaagata gcaaatcaaa ctgcgccatt ggtgtggcgc
     2521 tgaacgatac cgcctctaaa gtgttgcgtg atcaaacagg caagcatcac aaatgtgggt
     2581 gtacatacca gggcggctaa gcgagcagat ggaacatcaa tgcctgcgct caggaagatg
     2641 cgcattgaca gcaagacagc atggctatca gcttgtcgtc gtgcaggaat tgaaaatttc
     2701 cgtttccatg acctcagaca taccagggca agctggttga ttccgtcagg cgtcccatta
     2761 tcagtgcttc aggaaatggg cggatgggag tccagagaaa tggttcgtag atatgctcat
     2821 cttgcgccta atcatttgac tgagcacgcg aggaaaatag acgacatttt tggtgataat
     2881 gtcccactct ggaattatag aagcaataaa gaaggcgtaa ctgattgaat tttaatggcg
     2941 cgccctgcag gattcgaacc tgcggcccac gacttagaag gtcgttgctc tatccaactg
     3001 agctaagggc gcattgatac cacggtgtgc tgtaatcgcg tgaattatac ggtcaaccct
     3061 tgctgagtca atggcttttg aaccagttgc tgaacaagtg tacgaccgca tctgatgtag
     3121 ctcactatgg cggcaaacca acgacggtta tcatgatagg tgtgaatcag ccaggtacag
     3181 aaggttttac ttttcagaaa acgaaaactg acagcgcgcc acgcttctga caaaataggc
     3241 gcatcccctt cgacctacgt aacagatgga atcctctctc tgatggcagc aaagattatt
     3301 gacggtaaaa cgattgcgca gcaggtgcgc tctgaagttg ctcaaaaagt tcaggcgcgt
     3361 attgcagccg gactgcgagc accaggactg gccgttgtgc tggtgggcag taaccctgca
     3421 tcgcaaattt atgtcgcaag caaacgcaag gcttgtgaag aagtcgggtt cgtctcccgc
     3481 tcttatgacc tcccggaaac caccagcgaa gcggagctgc tggagcttat cgatgcgctg
     3541 aatgccgaca acaccattga tggcattctg gttcaactgc cgttaccggc gggtattgat
     3601 aacgtcaaag tgctggaacg tattcatccg gacaaagacg tggacggttt ccatccttac
     3661 aacgtcggtc gtctgtgcca gcgcgcgcca cgtctgcgtc cctgtacccc gcgcggtatc
     3721 gtcacgctgc ttgagcgtta caacattgat accttcggcc ttaacgccgt ggtgattggc
     3781 gcatcgaata tcgtcggtcg cccaatgagc atggaactgc tgctggcagg ttgcaccacc
     3841 accgtgactc accgtttcac taaaaatctg cgtcaccacg tagaaaatgc cgatctactg
     3901 atcgttgccg tcggcaagcc gggctttatt cctggtgact ggatcaaaga aggcgcaatt
     3961 gtgattgatg tcggcatcaa ccgtctggaa aatggcaaag ttgtgggcga cgtcgtgttt
     4021 gaagacgcgg ctaaacgcgc ctcatacatt acgcctgttc cgggcggcgt tggcccgatg
     4081 acggttgcca cgctgattga aaacacgcta caggcgtgcg ttgaatatca tgatccacag
     4141 ggtgagtaac atggcgacat tttctttagg taaacatccg cacgttgagc tgtgcgactt
     4201 gctgaaactg gaaggctgga gcgaaagcgg cgcgcaggcg aaaatcgcga tagcagaagg
     4261 ccaggtgaaa gtcgacggtg cggttgaaac gcgcaaacgc tgcaaaattg tcgccggtca
     4321 gacagtgagt tttgcaggtc acagcgtaca ggttgttgcc tgatcccttt cccggcctgt
     4381 atcaacaggc cggttctctt ttcagacttt tgccctctac gccccgttca tccatgctga
     4441 tagcatcgac attttgccga agcgaccatc atgccaaccg ttattacgca cgccgccgtt
     4501 cccctatgca ttggtttagg gttgggttcg aaagccatcc caccgcgtct tttatttgcc
     4561 ggaatcgtcc tggcgatgtt gcctgatgcc gacgtcttgt cgtttaaatt tggcgttgct
     4621 tacggcaatg tttttggtca tcgcgggttt acccattcgc tggtgtttgc gtttgttgtc
     4681 ccgctgttat gtgtgctgat tgggcgtcga tggttcaggg ctgggctgat tcgctgctgg
     4741 ctgtttttaa ccgtctcatt gctatcgcac agcttgctgg attcggtaac cactggtggt
     4801 aaaggcgttg gctggctatg gccgtggtca gatgaacgct ttttcgctcc ctggcaggtg
     4861 attaaagtcg cgccgtttgc attgtctcgt tacaccacgc cgtacgggca tcaggtgatt
     4921 atttctgaat tgatgtgggt gtggttgccg gggatgctac tgatgggaat gttgtggtgg
     4981 cgcaggcgat aaccggatgc gaaaactcgc atccggcaat agcgcaatta cttacgacgc
     5041 caggtggtcc cttgcgggcc atcttccagc acgatcccca tctcgttaag acggtcacgc
     5101 gccgcatctg ccgccgccca gtctttcgct ttacgggcat ccagacgctg ttgaattaac
     5161 gcttcaatct cagccacttc gctgtcgtct gcctgcgcac cgctttgcag gaatgcttcc
     5221 ggttcttgct ccagcagacc caatacggca gaaagtttac gcaggtgaga cgccattgca
     5281 ttcgccgctg ccatatcttc tgctttcaga cggttcactt cacgcgccat atcaaacagc
     5341 acggaatagg cttccggggt gttgaaatcg tcgtccattg cttcaataaa gcgcgcttca
     5401 aacgcttcgc caccagcagg cgcaacggtt ttgtctgtgc cgcgcaaggc agtatagaga
     5461 cgttccagcg ccgcacgcgc ctgtttcagg ttctcttcgc tgtagttcag ctggctgcga
     5521 tagtgacccg acatcaggaa gtaacgcaca gtttccgcat cgtagtattt cagcacgtca
     5581 cgcacggtga agaagttacc cagcgattta gacatcttct cgcggtcaac catcaccata
     5641 ccggagtgca tccagtaatt cacatactgg ccatcgtgcg cacaggtgga ctgggcaatt
     5701 tcgttctcgt ggtgcgggaa catcaggtca gaaccgccgc cgtggatatc aaagtggtta
     5761 cccaactgtt tgcagttcat tgccgaacac tcaatgtgcc agccaggacg gcccgcgccc
     5821 cacggagacg gccagctcgg ttcgccctct ttcgacatct tccacagaac gaagtccatc
     5881 gggttacgtt tgtcatcgac cacgtcaacg cgcgcgcctg cctgcagctg atcgagatcc
     5941 tgacgcgaca gcacgccata tgtcggatcg gtcgggacgt cgaacatcac gtcgccgttg
     6001 tccgccacat aagcgtgacc tttagcgatc agttgttcag tgagttcaat aatttcggcg
     6061 atatggtgcg tcgcgcgcgg ctccatatcc gggcgcagaa tgttcaaagc atcaaaatct
     6121 ttgtgcattt cggcgatcat gcgatccacc agcgccacaa agctttcgcc attttcattg
     6181 gcgcgtttga tgattttgtc gtcgatatcg gtaatgttgc gcacatactt cagcttatag
     6241 ccgaggaaac gcagatagcg cgcaaccacg tcaaaggcaa caaaggtacg cccgtgaccg
     6301 atatgacaga gatcgtaaac ggtgattcca cacacgtaca tgccgacttc cccggcgtga
     6361 ataggcttaa attcctcttt ttggcgtgtc agagtattga agatttttag catcgaagat
     6421 tccgtttaga catgtgtggg taattgagtt gcgtataata cccatatttc ccgcgcgaat
     6481 cagcatacat tgcgtgatga tccgatcgtg cggttatgct ataacaccac cctatatatg
     6541 acccgaaccg ggttgaagca ccaatcaaac ggaacaggat gcaaaaatgg ttactttcca
     6601 caccaatcac ggcgatattg tcatcaaaac ttttgacgat aaagcacctg aaacagttaa
     6661 aaacttcctg gactactgcc gcgaaggttt ttacaacaac accattttcc accgtgtaat
     6721 caacggcttt atgattcagg gcggcggttt tgaacctggc atgaagcaaa aaaccaccaa
     6781 agacccgatc aaaaacgaag ccaacaacgg tctgaaaaat acccgtggta cgctggcaat
     6841 ggcccgtact caggcaccgc actctgcaac tgcacagttc ttcatcaacg tggttgataa
     6901 cgacttcctg aacttctctg gcgaaagcct gcaaggttgg ggctactgcg tgtttgctga
     6961 agtggttgaa ggcatggacg tggtagacaa aatcaaaggt gttgcaaccg gtcgtagcgg
     7021 tatgcaccag gacgtgccaa aagaagacgt tatcattgaa agcgtgaccg ttagcgagta
     7081 atcgtggcga cactctttat tgcagatctt catctctgcg cggaagaacc ggcgatcacc
     7141 gccggttttc tgcgtttttt agcgggggaa gcccgcaagg ccgacgcgct gtatattctt
     7201 ggcgatctgt ttgaagcgtg gattggcgac gacgatccca acccactcca tcgccagatg
     7261 gcggcagcga tcaaagcggt atccgattcc ggcgttccct gttatttcat tcatggcaac
     7321 cgtgattttc tgctcggcaa acgctttgcc cgtgaaagcg gcatgacgtt attgccggaa
     7381 gaaaaggtgc tcgaacttta tggtcgtcgg gtgttgatta tgcatggcga cacgctgtgc
     7441 accgatgacg cgggttatca ggcttttcgc gccaaagtcc acaaaccctg gctgcaaacg
     7501 ctattcctcg ccctgccgtt gtttgtgcgc aaacgcattg ccgcgcgaat gcgcgcgaac
     7561 agcaaagaag ccaacagcag taaatcgctg gcgatcatgg acgttaacca aaacgcggtg
     7621 gtcagtgcga tggaaaaaca tcaagtgcaa tggctgatcc acgggcatac ccatcgcccg
     7681 gcggtgcatg aacttatcgc caatcagcaa cctgcttttc gcgtggtact gggtgcctgg
     7741 catacagaag gttcgatggt gaaagtcacg gcggatgacg ttgagctgat tcatttcccg
     7801 ttttaaaaaa accgcaactt tgctgatttc acagccacgc aaccgttttc cttgcactct
     7861 ttccgtgcta ttctctgtgc cctctaaagc cgagagttgt gcaccacagg agttttaaga
     7921 cgcatgtctt cccgcaataa tccggcgcgt gtcgccatcg tgatggggtc caaaagcgac
     7981 tgggctacca tgcagttcgc cgccgaaatc ttcgaaatcc tgaatgtccc gcaccacgtt
     8041 gaagtggttt ctgctcaccg cacccccgat aaactgttca gcttcgccga aagcgccgaa
     8101 gagaacggtt atcaggtgat tattgcgggc gcaggcggcg cagcgcacct gcctggcatg
     8161 attgccgcca aaacgctggt gccggtgctg ggcgtgccag tacagagcgc cgcactgagc
     8221 ggtgtcgata gcctctactc catcgtacaa atgccgcgcg gcattccggt gggtacgctg
     8281 gcgattggta aagctggcgc ggcaaacgcg gcgttactgg cagcacaaat tcttgcgact
     8341 catgataaag aactgcacca gcgtctgaat gaatggcgca aagcacaaac tgatgaagtg
     8401 ctggaaaacc cggacccgcg aggtgcggca tgaaacaggt ttgcgtcctc gggaacgggc
     8461 agttaggccg tatgctgcgt caggcaggcg aaccgttagg cattgctgtc tggccggtcg
     8521 ggctggacgc tgaaccggcg gcggtgcctt ttcaacaaag cgtgattacc gctgagatcg
     8581 aacgctggcc ggaaaccgca ttaacccgcg agctggcacg ccatccggcc tttgtgaacc
     8641 gcgatgtgtt cccgattatt gccgaccgtc tgactcagaa gcagcttttc gataaactcc
     8701 acctgccgac cgcaccgtgg cagttacttg ccgaacgcag cgagtggcct gcggtgtttg
     8761 atcgtttagg tgagctggcg attgttaagc gccgcactgg tggctatgac ggacgcggtc
     8821 aatggcgttt acgcgccgct gaaaccgaac agttaccggc tgaatgctac ggcgaatgta
     8881 ttgtcgagca gggcataaac ttctctggtg aagtgtcgct ggttggcgcg cgcggttttg
     8941 atggcagcac cgtgttttat ccgctgacgc ataacctgca tcaggacggc attttgcgca
     9001 ccagcgtcgc tttcccgcag gccaacgcgc agcagcaggc gcaggccgaa gagatgctgt
     9061 cggcgattat gcaggagctg ggctatgtgg gcgtgatggc gatggagtgt tttgtcaccc
     9121 cgcaaggtct gctgatcaac gaactggctc cgcgtgtgca taacagcggt cactggacac
     9181 aaaacggtgc cagcatcagc cagtttgagc tgcatctgcg ggcgattacc gatctgccgt
     9241 taccgcaacc ggtagtgaat agtccgtcgg tgatgatcaa cctgattggt agcgatgtga
     9301 attatgactg gctgaaactg ccgctggtgc atctgcactg gtacgacaaa gaagtccgtc
     9361 cggggcgtaa agtgggacat ctgaatttga ccgacagcga cacatcgcgt ctgaccgcga
     9421 cgctggaagc cttaatcccg ctgctgccgc cagagtatgc cagcggcgtg atgtgggcgc
     9481 agagtaagtt cagttaattg atgctcattt tcttgccgca tgcggcgtga acgccttatc
     9541 cggcctaccg ttccaacacc gactgtaggc c
//
LOCUS       DADSYM010000162         9517 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2415
            SAMN08039579-rid11723173.denovo.164, whole genome shotgun sequence.
ACCESSION   DADSYM010000162 DADSYM010000000
VERSION     DADSYM010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039579
            Sequence Read Archive: SRR6362533
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9517)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9517)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980162.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 03:39:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,484
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 4,347
            CDSs (with protein)               :: 4,347
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 43 of 85
            Pseudo Genes (incomplete)         :: 37 of 85
            Pseudo Genes (internal stop)      :: 19 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9517
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039579-rid11723173.denovo.164"
                     /strain="LGE2415"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8302 N 95.9628 W"
                     /collection_date="2015-08-24"
     gene            complement(<1..620)
                     /gene="gloB"
                     /locus_tag="J1Y83_003758"
     CDS             complement(<1..620)
                     /gene="gloB"
                     /locus_tag="J1Y83_003758"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase"
                     /protein_id="HBA7631866.1"
                     /translation="MNLNSIPAFDDNYIWVLNDEAGRCLIVDPGDAEPVLNAISANNW
                     QPEAIFLTHHHHDHVGGVKELVEKFPQIVVYGPQETQDKGTTQVVKDGETAFVLGHEF
                     SVIATPGHTLGHICYFSKPYLFCGDTLFSGGCGRLFEGTPSQMYQSLKKLSALPDDTL
                     VCCAHEYTLSNMKFALSILPHDLSINDYYRKVKELRAKNQITLPVIL"
     gene            654..1376
                     /locus_tag="J1Y83_003759"
     CDS             654..1376
                     /locus_tag="J1Y83_003759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414749.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HBA7631867.1"
                     /translation="MKPARVPQTVVAPDCWGDLPWGELYRKALERQLNPWFTKMYGFH
                     LLKIGNLSAEINCEACAVSHQVNVSAQGMPVQVQADPLHLPFADKSVDVCLLAHTLPW
                     CTDPHRLLREADRVLIDDGWLVISGFNPISLMGLRKLVPVLRKTSPYNSRMFTLMRQL
                     DWLSLLNFEVLHASRFHVLPWNKHGGKLLNAHIPALGCLQLIVARKRTIPLTLNPMKQ
                     SKNKPRIRQAVGATRQCRKPQA"
     gene            complement(1373..1840)
                     /gene="rnhA"
                     /locus_tag="J1Y83_003760"
     CDS             complement(1373..1840)
                     /gene="rnhA"
                     /locus_tag="J1Y83_003760"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI"
                     /protein_id="HBA7631868.1"
                     /translation="MLKQVEIFTDGSCLGNPGPGGYGAILRYRGYEKTFSAGYTRTTN
                     NRMELMAAIVALEALKEHCEVILSTDSQYVRQGITQWIHNWKKRGWKTADKKPVKNVD
                     LWQRLDAALGQHQIKWEWVKGHAGHPENERCDELARAAAMNPTLEDTGYQVEV"
     gene            1905..2636
                     /gene="dnaQ"
                     /locus_tag="J1Y83_003761"
     CDS             1905..2636
                     /gene="dnaQ"
                     /locus_tag="J1Y83_003761"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit epsilon"
                     /protein_id="HBA7631869.1"
                     /translation="MSTAITRQIVLDTETTGMNQIGAHYEGHKIIEIGAVEVVNRRLT
                     GNNFHVYLKPDRLVDPEAFGVHGIADEFLLDKPTFAEVADEFMDYIRGAELVIHNATF
                     DIGFMDYEFSLLKRDIPKTNTFCKVTDSLAVARKMFPGKRNSLDALCARYEIDNSKRT
                     LHGALLDAQILAEVYLAMTGGQTSMAFAMEGETQQQQGETTIQRIVRQASKLRVVFAT
                     DEELAAHEARLDLVQKKGGSCLWRA"
     gene            2769..2845
                     /locus_tag="J1Y83_003762"
     tRNA            2769..2845
                     /locus_tag="J1Y83_003762"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2803..2805,aa:Asp,seq:gtc)
     gene            complement(2944..3714)
                     /gene="yafV"
                     /locus_tag="J1Y83_003763"
     CDS             complement(2944..3714)
                     /gene="yafV"
                     /locus_tag="J1Y83_003763"
                     /EC_number="3.5.1.111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutaramate amidase"
                     /protein_id="HBA7631870.1"
                     /translation="MPGLKITLLQQPLVWMDGPANLRHFDRQLEGITGRDVIVLPEMF
                     TSGFAMEAAASSLAQDDVVNWMTAKAQQCNALIAGSVALQTESGSVNRFLLVEPGGTV
                     HFYDKRHLFRMADEHLHYKAGNVRVIVEWRGWRILPLVCYDLRFPVWSRNLNDYDLAI
                     YVANWPAPRSLHWQALLTARAIENQAYVAGCNRVGSDGNGCHYRGDSRVINPQGEIIA
                     TADAHQATRIDAELSMMALREYREKFPAWRDADEFTFR"
     gene            3868..4341
                     /gene="ivy"
                     /locus_tag="J1Y83_003764"
     CDS             3868..4341
                     /gene="ivy"
                     /locus_tag="J1Y83_003764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C-lysozyme inhibitor"
                     /protein_id="HBA7631871.1"
                     /translation="MGRISPGGMMFKAITTVAALVIATSAMAQDDLTISSLAKGETTK
                     AAFNQMVQGHKLPAWVMKGGTYTPAQTVTLGDETYQVMSACKPHDCGSQRIAVMWSEK
                     SNQMTGLFSTIDEKTSQEKLTWLNVNDALSIDGKTVLFAALTGSLENHPDGFNFK"
     gene            complement(4384..6828)
                     /locus_tag="J1Y83_003765"
     CDS             complement(4384..6828)
                     /locus_tag="J1Y83_003765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414756.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HBA7631872.1"
                     /translation="MMILSILATVVLLGALFYHRVSLFISSLILLAWTAALGVAGLWS
                     AWVLVPLAIILVPFNFAPMRKSMISAPVFRGFRKVMPPMSRTEKEAIDAGTTWWEGDL
                     FQGKPDWKKLHNYPQPRLTAEEQAFLDGPVEEACRMANDFQITHELADLPPELWAYLK
                     EHRFFAMIIKKEYGGLEFSAYAQSRVLQKLSGVSGILAITVGVPNSLGPGELLQHYGT
                     DEQKNHYLPRLARGQEIPCFALTSPEAGSDAGAIPDTGIVCMGEWQGQQVLGMRLTWN
                     KRYITLAPIATVLGLAFKLSDPEKLLGAAEDLGITCALIPTTTPGVEIGRRHFPLNVP
                     FQNGPTRGKDVFVPIDYIIGGPKMAGQGWRMLVECLSVGRGITLPSNSTGGVKSVALA
                     TGAYAHIRRQFKISIGKMEGIEEPLARIAGNAYVMDAAASLITYGIMLGEKPAVLSAI
                     VKYHCTHRGQQSIIDAMDITGGKGIMLGQSNFLARAYQGAPIAITVEGANILTRSMMI
                     FGQGAIRCHPYVLEEMEAAKNNDVNAFDKLLFKHIGHVGSNKVRSFWLGLTRGLTSST
                     PTGDATKRYYQHLNRLSANLALLSDVSMAVLGGSLKRRERISARLGDILSQLYLASAV
                     LKRYDDEGRNEADLPLVHWGVQDALYQAEQAMDDLLQNFPNRVVAGLLNVVIFPTGRH
                     YLAPSDKLDHKVAKILQVPNATRSRIGRGQYLTPSEHNPIGLLEEALVDVIAADPIHQ
                     RICKELGKNLPFTRLDELAHNALAKGLIDKDEAAILVKAEESRLRSINVDDFDPEELA
                     TKPVKLPEKVRKVEAA"
     gene            7068..7646
                     /gene="lpcA"
                     /locus_tag="J1Y83_003766"
     CDS             7068..7646
                     /gene="lpcA"
                     /locus_tag="J1Y83_003766"
                     /EC_number="5.3.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-sedoheptulose 7-phosphate isomerase"
                     /protein_id="HBA7631873.1"
                     /translation="MYQDLIRNELNEAAETLANFLKDDANIHAIQRAAVLLADSFKAG
                     GKVLSCGNGGSHCDAMHFAEELTGRYRENRPGYPAIAISDVSHISCVGNDFGFNDIFS
                     RYVEAVGREGDVLLGISTSGNSANVIKAIAAAREKGMKVITLTGKDGGKMAGTADIEI
                     RVPHFGYADRIQEIHIKVIHILIQLIEKEMVK"
     gene            7851..8618
                     /locus_tag="J1Y83_003767"
     CDS             7851..8618
                     /locus_tag="J1Y83_003767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II glutamine amidotransferase"
                     /protein_id="HBA7631874.1"
                     /translation="MCELLGMSANVPTDICFSFTGLVQRGGGTGPHKDGWGITFYEGK
                     GCRTFKDPQPSFNSPIAKLVQDYPIKSCSVLAHIRQANRGEVALENTHPFTRELWGRN
                     WTYAHNGQLTGYKSLETGNFRPVGETDSEKAFCWLLHKLTQRYPRTPGNMAAVFKYIA
                     SLADELRQKGVFNMLLSDGRYVMAYCSTNLHWITRRAPFGVATLLDQDVEIDFSSQTT
                     PNDVVTVIATQPLTGNETWQKIMPGEWRLFCLGERVV"
     gene            complement(8589..9329)
                     /locus_tag="J1Y83_003768"
     CDS             complement(8589..9329)
                     /locus_tag="J1Y83_003768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein L,D-transpeptidase"
                     /protein_id="HBA7631875.1"
                     /translation="MRKIALILAMLLIPCVSFAGLLGSSSSTTPVSKEYKQQLMGSPV
                     YIQIFKEERTLDLYVKMGEQYQLLDSYKICKYSGGLGPKQRQGDFKSPEGFYSVQRNQ
                     LKPDSRYYKAINIGFPNAYDRAHGYEGKYLMIHGDCVSIGCYAMTNQGIDEIFQFVTG
                     ALVFGQPSVQVSIYPFRMTDANMKRHKYSNFKDFWEQLKPGYDYFEQTRKPPTVSVVN
                     GRYVVSKPLSHEVVQPQLASNYTLPEAK"
BASE COUNT         2343 a         2468 c         2398 g         2308 t
ORIGIN      
        1 agaattacgg gtagtgttat ttgatttttt gcccgtaact ccttaacttt acgataataa
       61 tcatttatgg acaaatcgtg cggaagaata ctcaaagcaa acttcatatt tgataaggta
      121 tattcatgag cacaacatac caatgtatcg tcaggtaacg cacttaactt tttaagtgat
      181 tgatacattt gtgatggtgt cccttcaaac aaccgaccac acccaccaga aaacagcgtg
      241 tcgccgcaaa atagataagg tttactgaag taacagatat gtcctaaagt gtgacccggt
      301 gtggcaatta cactaaattc atgccccaaa acgaaggcag tttcgccatc tttgactacc
      361 tgtgttgttc ccttatcttg tgtctcttgt ggtccataca ccacaatttg cggaaacttt
      421 tccaccagtt cttttacgcc gccaacgtga tcgtggtgat ggtgggtgag aaatatggcc
      481 tccggttgcc agttattggc ggaaatggcg tttaatactg gctctgcgtc cccgggatcg
      541 acaatcagac agcgacctgc ttcatcattc aaaacccaga tgtaattgtc atcaaaggcg
      601 ggaatactgt taagattcat agattacctc tcagtgtgaa acggaaggtt gtgatgaaac
      661 cggcaagagt ccctcaaact gtcgtggctc ctgattgctg gggcgatttg ccctggggag
      721 agctttatcg caaggcgctg gagcgtcagc tcaacccgtg gttcactaaa atgtatggtt
      781 ttcatctgct taagattggc aatttaagcg cagaaatcaa ttgcgaagcg tgcgcggttt
      841 ctcatcaagt gaatgtttct gcgcaaggaa tgcccgtcca ggtgcaggcg gacccacttc
      901 atcttccttt tgccgataaa tccgttgatg tttgtctact ggcacataca ttgccgtggt
      961 gcactgatcc gcatcgttta ttgcgtgaag ccgatcgggt actgattgat gatggctggc
     1021 tggtgattag tggcttcaat cccatcagtt taatgggatt acgcaaactt gtgccggtat
     1081 tgcgcaaaac ctcgccctat aacagccgga tgtttactct gatgcggcag ctggactggc
     1141 tctctttgtt gaattttgaa gtgctacacg ccagccgttt ccacgttctc ccgtggaaca
     1201 aacacggagg aaaactattg aatgcgcata ttcctgcgct tggttgttta caacttattg
     1261 ttgcccggaa acggactatt cctttaacgc taaatccgat gaaacagagc aagaataaac
     1321 cgcgaattcg ccaggcggtt ggtgccaccc ggcaatgtcg taaaccacag gcttaaactt
     1381 cgacttgata gcctgtatct tccagtgtgg gattcattgc cgcagcacga gccagttcat
     1441 cacagcgttc gttttccggg tgtccggcat ggcctttaac ccattcccat ttgatttgat
     1501 gctgccccag agcagcatca agacgttgcc agagatcgac attttttact ggttttttgt
     1561 ctgctgtttt ccagccacgt tttttccagt tatggatcca ctgggtgata ccctggcgga
     1621 catactggct gtcggtactc aaaatgactt cgcaatgttc ttttaacgcc tccagcgcga
     1681 caatagcggc catcaactcc atacggttgt tggtggtgcg ggtgtagcca gcgctaaagg
     1741 ttttctcgta tccgcgatag cgtaaaatag cgccgtaacc cccaggtcca ggattaccca
     1801 gacacgaacc atcggtgaaa atttctacct gtttaagcat ctctggtaga cttcctgtaa
     1861 ttgaatcgaa ctgtaaaacg acaagtctga cataaatgac cgctatgagc actgcaatta
     1921 cacgccagat cgttctcgat accgaaacca ccggtatgaa ccagattggt gcgcactatg
     1981 aaggccacaa gatcattgag attggtgccg ttgaagtggt gaaccgtcgc ctgacgggca
     2041 ataacttcca tgtttatctc aaacccgatc ggctggtgga tccggaagcc tttggcgtac
     2101 atggtattgc cgatgaattt ttgctcgata agcccacgtt tgccgaggta gccgatgagt
     2161 tcatggacta tatccgcgga gcggagctgg tgatccataa cgcaacgttc gatatcggct
     2221 ttatggacta cgagttttcg ttgcttaagc gcgatattcc gaagaccaat accttctgta
     2281 aggtcaccga tagccttgcg gtggcgagga aaatgtttcc cggtaaacgc aacagcctcg
     2341 atgcgttatg tgcccgctac gaaatagata acagtaaacg aacgctgcat ggggcattac
     2401 tcgatgccca gatccttgcg gaagtttatc tggcgatgac aggtggtcaa acgtcgatgg
     2461 cttttgcgat ggaaggagag acacaacagc aacaaggtga aacaacaatt cagcgcattg
     2521 tgcgtcaggc aagtaagtta cgcgttgttt ttgcgacaga tgaagagctt gcagcacatg
     2581 aagcccgtct cgatctggtg cagaagaagg gcggaagctg cctctggcgg gcataaatac
     2641 ctgtaaaagg cgctaaaaat agcgacttgg gcgatttttg cagcaaacga ttcaaaagat
     2701 gagaaaaacc gttgacgaag ggtgaggcaa tccgtaatat tcgcctcgtt cccaacggaa
     2761 cacaacgcgg agcggtagtt cagtcggtta gaatacctgc ctgtcacgca gggggtcgcg
     2821 ggttcgagtc ccgtccgttc cgccaatatt cagaaagccc gaatcattac tgattcgggc
     2881 tttcgtcgtt taagttccag agaaaaatag ctacgaaact tatctcgtag ccatattcac
     2941 cctctaacga aatgtgaatt catccgcatc ccgccatgcc ggaaactttt ctctatattc
     3001 ccgcaatgcc atcatcgaca gctccgcatc aatgcgcgtt gcctgatgcg cgtcggcagt
     3061 agcaataatc tcgccctgtg gattaatcac ccggctgtca ccgcgataat ggcagccgtt
     3121 gccatcgctg ccgacgcgat tgcatcccgc cacatacgcc tgattctcaa tcgcgcgggc
     3181 tgtcagcaat gcctgccagt gcagagagcg tggggcaggc cagttggcga cgtagatggc
     3241 gaggtcataa tcgttgagat tgcgcgacca caccggaaaa cgtaagtcgt agcacaccag
     3301 cggcaaaata cgccagccgc gccattccac aatcactcgc acattgcccg ctttataatg
     3361 cagatgctca tctgccattc ggaacagatg acgcttatca taaaaatgta ccgtgccgcc
     3421 cggctcaacc agcaaaaagc ggttaaccga accagactcc gtttgtaatg caacactgcc
     3481 tgcaatcagc gcattgcact gctgcgcttt ggctgtcatc cagttcacta cgtcatcttg
     3541 tgctagcgac gaagctgccg cttccatggc aaagccgctg gtaaacatct ccggtagaac
     3601 gatcacatcg cgcccggtaa taccttccag ttgacgatca aaatggcgca ggttggcagg
     3661 gccatccatc cacaccagtg gttgctgcaa aagcgtaatc ttcaaaccag gcacggtgta
     3721 caactccttt atgcgaaggg ttttataact ctaacacctt atcaggcagt tgccttagcg
     3781 cagaataaat tgataacaaa tgctgatatt ggaaatatct gatttgcaaa ttatcgtgtt
     3841 atcgccaggc tttagggagt taatcacatg ggcaggataa gcccgggagg aatgatgttt
     3901 aaggcaataa cgacagtcgc cgctctggtc atcgccacca gtgcaatggc gcaggatgat
     3961 ttaaccatta gcagtcttgc aaagggcgaa accaccaaag cggcgtttaa tcagatggtg
     4021 caagggcata agctgcctgc ctgggtgatg aaaggcggta cttatactcc cgcacaaacc
     4081 gtgacattgg gagatgagac gtatcaggtg atgagcgcgt gcaaaccgca tgactgtggc
     4141 tcgcaacgta tcgctgtgat gtggtccgag aaatctaatc agatgacggg gctgttctcg
     4201 accattgatg agaaaacgtc gcaagagaaa ctcacctggt tgaatgtgaa cgatgcgctt
     4261 tctattgatg gtaaaacggt gctgttcgcg gcgttgaccg gcagcctgga aaaccatccg
     4321 gatggcttta attttaaata attagccgat aaaaaaacgg agccgaaagg ctccgttatt
     4381 caattacgcg gcttcaactt tccgcacttt ctccggcaac tttaccggct tcgtcgccag
     4441 ctcttctgga tcaaagtcat caacgttaat actacgcaga cggctttctt cagctttcac
     4501 cagaatagcg gcttcatctt tatcaatcag ccccttcgcc agcgcgttgt gcgccagttc
     4561 atccagacgg gtaaacggca ggtttttacc caactcttta cagatccgct gatgaattgg
     4621 gtcggcggca atcacatcca ccagcgcctc ttccagtaaa ccaatcgggt tatgctcgct
     4681 cggggtcagg tactgaccgc gaccaatacg tgaacgggtg gcgttcggca cttgtaaaat
     4741 cttcgccact ttatgatcca gcttgtcaga cggtgccaga taatgacgtc cggtcgggaa
     4801 gatcaccaca ttcagcagcc cggcaaccac gcggttcggg aagttttgca gtaaatcgtc
     4861 catcgcctgt tctgcctgat acagcgcatc ctgtacgccc cagtgcacca gcggcagatc
     4921 ggcttcatta cggccttcgt cgtcataacg cttcagaacg gcagaggcga ggtaaagctg
     4981 gcttaaaata tcacccagac gtgccgagat acgctcacga cgtttcaggc tgccgcccag
     5041 caccgccatc gagacatcag aaagcagggc gaggttggcg ctcaggcggt tcaggtgctg
     5101 atagtagcgt ttagtggcat cgccggttgg tgtgctgctg gttaaaccgc gcgtcaggcc
     5161 cagccagaag ctgcgaactt tgttgctacc gacgtgaccg atatgtttaa acaggagttt
     5221 atcgaacgcg ttgacgtcat tgttcttcgc cgcttccatc tcttccagca cgtacggatg
     5281 gcaacgaatc gccccctgac cgaaaatcat catgctacgg gtcagaatat ttgccccttc
     5341 aacggtgatg gcaatcggcg cgccctgata agcacgcgcc aggaagttgc tctgcccgag
     5401 cataatgcct ttgccaccag tgatatccat cgcatcaata atcgactgct gcccgcggtg
     5461 ggtacagtga tacttaacga tagccgacag cacggcaggt ttttcaccga gcataatgcc
     5521 gtaggtaatc agcgatgccg cagcatccat tacataagcg tttccggcaa tacgcgccag
     5581 cggctcttca atcccttcca tcttaccgat agagattttg aactgacgac gaatgtgtgc
     5641 atacgcgccg gttgccagcg ctaccgattt cacgccgccg gttgagttgg aaggcagggt
     5701 gatgccgcgg cctaccgaga ggcactccac cagcatccgc cagccttgcc cggccatttt
     5761 cggcccgccg atgatgtaat cgatcggcac gaacacatct ttacctcgcg tcggtccgtt
     5821 ctggaacggt acgttcagcg ggaagtggcg acgaccaatt tccacgcccg gcgtggtggt
     5881 cgggatcagc gcacaggtaa tacctaaatc ttctgcagcg ccgagtaatt tctccgggtc
     5941 ggagagttta aacgccagac caagcacggt cgcaatcggt gccagcgtaa tgtagcgttt
     6001 gttccaggtc agacgcatcc ccagcacctg ctggccctgc cattcgccca tgcagacaat
     6061 cccggtatcc ggaatcgcgc ccgcatcgga acccgcttcg gggctggtca gtgcaaagca
     6121 agggatctcc tgaccacgcg ccagacgcgg cagatagtga tttttctgct cgtcagtgcc
     6181 gtaatgttgc agcagctcgc ccgggcctaa tgagttcggc acgccgacgg taattgccag
     6241 gatcccgctc acgccggaga gtttttgcag cacacgagat tgggcataag ccgagaactc
     6301 cagcccgccg tactcttttt tgatgatcat cgcgaagaaa cgatgctctt taaggtacgc
     6361 ccacaactcc ggcggcaggt ccgccagctc atgagtaatc tggaaatcat tcgccatccg
     6421 gcaggcttct tctaccggac cgtcgagaaa cgcttgctct tcagcggtca ggcgcggctg
     6481 cggatagtta tgcagctttt tccagtccgg cttgccctgg aacaagtcgc cctcccacca
     6541 ggtggtgccc gcatcaatcg cttctttctc agtgcgcgac atcggcggca tcaccttacg
     6601 gaaaccgcga aataccggtg cggaaatcat cgacttacgc ataggcgcaa agttaaatgg
     6661 cacgaggata atggccagag gcactagtac ccacgccgac cacagaccag caacgccgag
     6721 tgcggctgtc caggcgagca aaatcagact gctgataaat aagctcacgc ggtgatagaa
     6781 caacgcgccg agcaggacaa ccgtagcgag aatactcaaa atcatcataa cgaaaagccc
     6841 cttacttgta ggaggtctga ccacttgtga tgatatggtt gtagtggatg taaaaacatt
     6901 tagcaatgtg tttacaatat aattacaaca aagctcacat tgttgctgtt tttatccgca
     6961 cttcgggtca aaaagtcctg gtcatagcac ctgcccgtac ttctcgcttt tggcggtatc
     7021 cggtacactg cattttgtct attacattta tgctgaagga tatcctcatg taccaggatc
     7081 ttattcgtaa cgaactgaac gaagcggcgg aaacgctggc taacttttta aaagatgacg
     7141 ccaatattca cgccattcag cgcgcggcgg tcctgttagc agacagcttt aaagccggtg
     7201 gcaaagtgct ttcctgtggc aacggcggct cccattgcga cgccatgcac tttgccgaag
     7261 agttgaccgg tcgctaccgt gaaaaccgtc cgggctaccc ggcgattgcc atttctgacg
     7321 tcagccatat ttcctgcgtc ggtaatgatt ttggtttcaa tgatattttc tcccgttacg
     7381 ttgaagcggt aggtcgcgaa ggcgatgtac tgctggggat ctccacttcc ggtaactctg
     7441 caaacgtgat caaagcgatc gcggcggcgc gtgagaaggg gatgaaagtg atcaccctga
     7501 ccggtaaaga cggcggcaaa atggctggca cggcggatat tgaaattcgc gttccgcact
     7561 ttggttatgc cgaccgcatt caggagatcc acattaaagt gatccatatc ctgatccagt
     7621 taattgaaaa agagatggtt aagtaagtct ggcgtaggcc ggataaggcg tttacgccgc
     7681 atccggcatt tgtgctctga tgcctgatgc gacgctgacg cgtcttatca tgcctacaat
     7741 ctgcacccga accgtaggcc gaataatgcg ttcacgccac atccgatctg aaaattctta
     7801 aatcaatctt cgccgggggc catgcgctcc cgctgttgtg gaggttaccc atgtgcgaat
     7861 tgctcgggat gagcgccaac gtccctaccg atatctgctt tagtttcacc ggacttgtac
     7921 agcgtggtgg tggaaccggg ccacataaag atggctgggg cattaccttt tacgaaggta
     7981 aaggctgtcg cacatttaaa gatccgcaac ccagctttaa ttcccccatt gccaaacttg
     8041 tccaggacta cccgataaaa tcctgttcgg tgttggctca tattcgccag gctaatcggg
     8101 gcgaggtggc gctggaaaat actcacccgt ttacccgcga gttatggggg cgtaactgga
     8161 cttatgccca taatgggcaa ctgacgggct acaaatcact ggaaaccggc aacttccgcc
     8221 cggtcggtga aaccgatagc gaaaaagcct tttgctggct cctgcataaa ttaacgcagc
     8281 gttacccgcg cacaccgggc aacatggcgg cagtgtttaa atatatcgcc tcactggcgg
     8341 atgaactgcg gcagaagggc gttttcaaca tgctactttc ggatgggcgc tatgtaatgg
     8401 cgtattgctc gactaattta cactggatca cccgccgcgc gccgtttggc gtggcaacgt
     8461 tgctggatca ggatgtggaa atcgacttca gctcgcagac cacaccgaat gatgtggtca
     8521 cggtgattgc gacacagccg ctgacgggca atgaaacctg gcaaaagatt atgccaggtg
     8581 aatggcgctt attttgcctc ggggagcgtg tagtttgatg ccagttgtgg ctgcacaact
     8641 tcgtggctta acggcttgct gaccacgtaa cggccattga ccacagaaac ggtcggtggc
     8701 ttacgggttt gctcaaagta gtcgtagccc ggcttcagtt gctcccagaa gtccttaaag
     8761 ttggaatatt tatggcgctt catattggcg tcggtcatgc ggaacggata aatactcact
     8821 tgcacgctcg gctgaccaaa caccagcgca ccagtaacga actggaagat ctcatcaata
     8881 ccctgattgg tcatcgcgta gcagccgatg gaaacacaat cgccgtgaat catcaggtat
     8941 ttcccttcat aaccatgcgc acggtcatag gcatttggga atccaatatt aatcgctttg
     9001 tagtaacggc tgtctggttt taactgatta cgctggacgc tataaaaccc ttccgggctt
     9061 ttgaaatcgc cctgacgctg ttttggccct aagccgccgg aatatttaca gattttatag
     9121 ctgtcgagca gttgatattg ctcgcccatt ttgacgtaga gatcgagcgt acgttcttcc
     9181 ttgaagatct ggatgtagac aggggatccc atcaactgct gcttatactc tttgctcact
     9241 ggcgtggtcg agctactgct gcccagcaaa ccggcaaacg aaacgcacgg gatcaacaac
     9301 atcgcaagaa ttaatgcgat tttacgcata ctgcttattc cttgataaaa cggttacaca
     9361 cgccaggacg gcaaaatgaa tcccaaatcg gaatagtctg gatttggaag gctcacatta
     9421 tcaccaaaag agttttacgc aagcctgtcg gcgcagggtt tacaaattta gcacaggggc
     9481 agttttcaag agtccgaaaa gcgcatggat tgtagat
//
LOCUS       DADSYM010000163         9448 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2415
            SAMN08039579-rid11723173.denovo.165, whole genome shotgun sequence.
ACCESSION   DADSYM010000163 DADSYM010000000
VERSION     DADSYM010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039579
            Sequence Read Archive: SRR6362533
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9448)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9448)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980162.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 03:39:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,484
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 4,347
            CDSs (with protein)               :: 4,347
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 43 of 85
            Pseudo Genes (incomplete)         :: 37 of 85
            Pseudo Genes (internal stop)      :: 19 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9448
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039579-rid11723173.denovo.165"
                     /strain="LGE2415"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8302 N 95.9628 W"
                     /collection_date="2015-08-24"
     gene            30..824
                     /locus_tag="J1Y83_003769"
     CDS             30..824
                     /locus_tag="J1Y83_003769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001556839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBA7631876.1"
                     /translation="MKKPLVIISACQFTRLALESLIPTDRYIVRVYSNVTTEVEFVLK
                     TSCGYLLADIPSLPSGELVLLACLVRLRSDAGQWQVCLTGDSDWFDNAPAASLYNGFP
                     RIGTMLTASRYQNQIIRWLLRPLPGDMSDFWLLTARELSVLKILMQGMSFSEIARYEQ
                     RSSKTLHAIATRALMKLGLGTLSDFRLLYTGCGDSRVKRNIRLHARYVGQHSAHRLTQ
                     YVQNMVSGILYPEKVAVGESINSDAISGKVLSGQRKNISKKRPGLL"
     gene            978..1739
                     /locus_tag="J1Y83_003770"
     CDS             978..1739
                     /locus_tag="J1Y83_003770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA7631877.1"
                     /translation="MRFVLFCPSGIVPAQFAALSTGSVGNVTCIRTEEELRNKLRHRP
                     QNVVISAGRPAECAEMWFRFYRDHSFVVVLCVAPFFLPPDVSISGVLKNLRLLKPGMS
                     VERVISIANTSGGFSGLKHAEILPVMDSYSVFMKEVNNCTKTIVMSERFPEKQKKVLS
                     LLLAGHSWEYSAQFLKTGIRQIWLAEQSLKKRWGIPDSMSLREALLLSSNNFHGDGNA
                     LETTNAMTLREDGNTNRYSVVVNAGTQALSLHKYK"
     gene            1781..2863
                     /locus_tag="J1Y83_003771"
                     /pseudo
     CDS             1781..2863
                     /locus_tag="J1Y83_003771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308377.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
     gene            2885..4078
                     /locus_tag="J1Y83_003772"
     CDS             2885..4078
                     /locus_tag="J1Y83_003772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HBA7631878.1"
                     /translation="MTKATVNTGTFASQNGTLIVDASSENTLDISGKASGNLSVYSAG
                     SLDLINEQTAFISTGKDSTLKATGTTEGGLYQYDLTQGADGNFYFVKNTHKASNASSV
                     IQAMAAAPANVANLQADTLSARQDAVRLSENDKGGVWIQYFGGKQKHTTAGNASYDLD
                     VNGVMLGGDTRFMTEDGSWLAGVAMSSAKGDMTTMQSKGDTEGYSFHAYLSRQYNNGI
                     FIDTAAQFGHYSNTADVRLMNGGGTIKADFNTNGFGAMVKGGYTWKDGNGLFIQPYAK
                     LSALTLEGVDYQLNGVDVHSDSYNSVLGEAGTRVGYDFAVGNATVKPYLNLAALNEFS
                     DGNKVRLGDESVNASIDGAAFRVGAGVQADITKNMGAYASLDYTKGDDIENPLQGVVG
                     INVTW"
     gene            4263..4924
                     /locus_tag="J1Y83_003773"
                     /pseudo
     CDS             4263..4924
                     /locus_tag="J1Y83_003773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414844.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(5167..5862)
                     /locus_tag="J1Y83_003774"
     CDS             complement(5167..5862)
                     /locus_tag="J1Y83_003774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414842.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein C"
                     /protein_id="HBA7631879.1"
                     /translation="MDNRSEFLNNVAQALGRPLRLEPQAEDAPLNNYANERLTQLTQQ
                     QRCDAFIQFASDVMLTRCELTSEAKAAEAAIRLCKELGDQSVVISGDTRLEELGISER
                     LQQECNAVVWDPAKGIENISQAEQAKVGVVYAEYGLTESGGVVLFSAAGRGRSLSLLP
                     ESSLFILRKSTILPRVAQLAEKLHQKAQAGERMPSCINIISGPSSTADIELIKVVGVH
                     GPVKAVYLIIEDC"
     gene            complement(5855..7282)
                     /locus_tag="J1Y83_003775"
     CDS             complement(5855..7282)
                     /locus_tag="J1Y83_003775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster-binding protein"
                     /protein_id="HBA7631880.1"
                     /translation="MSIKTSNTDFKTRIRQQIEDPIMRKAVANAQQRIGANRQKMVDE
                     LGHWEEWRDRAAQIRDHVLSNLDAYLYQLSEKVTQNGGHVYFAKTKEDATRYILQVAQ
                     RKNARKVVKSKSMVTEEIGVNHVLQDAGIQVIETDLGEYILQLDQDPPSHVVVPAIHK
                     DRHQIRRVLHERLGYEGPETPEAMTLFIRQKIREDFLSAEIGITGCNFAVAETGSVCL
                     VTNEGNARMCTTLPKTHIAVMGMERIAPTFAEVDVLITMLARSAVGARLTGYNTWLTG
                     PREAGHVDGPEEFHLVIVDNGRSEVLASEFRDVLRCIRCGACMNTCPAYRHIGGHGYG
                     SIYPGPIGAVISPLLGGYKDFKDLPYACSLCTACDSVCPVRIPLSKLILRHRRVMAEK
                     GITAKAEQRAIKMFAYANSHPGLWKVGMMAGAHAASWFINGGKTPLKFGAISDWMEAR
                     DLPEADGESFRSWFKKHQAQEKKNG"
     gene            complement(7293..8012)
                     /locus_tag="J1Y83_003776"
     CDS             complement(7293..8012)
                     /locus_tag="J1Y83_003776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HBA7631881.1"
                     /translation="MNVNFFVTCIGDALKSRMARDSVLLLEKLGCRVNFPEKQGCCGQ
                     PAINSGYIKEAIPGMKNLIAALEDNDDPIISPAGSCTYAVKSYPTYLADEPEWASRAE
                     KVAARMQDLTSFIVNTLGVVDVGASLQGRAVYHPSCSLARKLGVKDEPLTLLKNVRGL
                     ELLTFAEQDTCCGFGGTFSVKMAEISGEMVKEKVAHLMEVRPEYLIGADVSCLLNISG
                     RLQREGQKVKVMHIAEVLMSR"
     gene            complement(8539..9393)
                     /locus_tag="J1Y83_003777"
     CDS             complement(8539..9393)
                     /locus_tag="J1Y83_003777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HBA7631882.1"
                     /translation="MDALSRLLMLNAPQGTIDKNCVLGSDWQLPHGAGELSVIRWHAL
                     TQGAAKLEMPTGEIFTLRPGNVVLLPQNSAHRLSHVDNESTCIVCGTLRLQHSARYFL
                     TSLPETLFVAPVNHSVEYNWLREAIPFLQQESRSAMPGVDALCSQICATFFTLAVREW
                     IAQVNTEKNILSLLLHPRLGAVIQQMMEMPGHAWTVESLASIAHMSRASFAQLFRDVS
                     GTTPLAVLTKLRLQIAAQMFSREMLPVVVIAESVGYASESSFHKAFVREFGCTPGEYR
                     ERVRQLAP"
BASE COUNT         2558 a         2261 c         2158 g         2471 t
ORIGIN      
        1 ttttgtagca atggcgtgga cgagtctgaa tgaagaagcc tctggttatt atcagtgcct
       61 gtcagttcac caggctggca ctggagtctc tcatcccaac ggacaggtat atcgtgcggg
      121 tgtacagcaa cgtcactaca gaggttgaat tcgttttaaa gacctcctgt ggctacctgt
      181 tagctgatat cccatcgctt ccttcgggag agttggttct tctggcttgt cttgtccgct
      241 tgcggagtga tgccgggcaa tggcaggtct gcctgaccgg tgactccgac tggttcgata
      301 atgctcctgc tgcaagtctg tataacggct tcccacgcat cggcaccatg ctgacagcga
      361 gtcgttatca gaatcaaatt atccgctggt tactacgtcc tttgcccggc gatatgagcg
      421 atttctggct cctgactgca cgggagttga gcgtgcttaa gattctgatg cagggcatga
      481 gtttcagtga gattgcccgt tatgaacaac gcagttcaaa gaccctgcat gcgattgcca
      541 cacgggcatt aatgaaactt ggactgggaa ctctgagcga tttcagactg ctctatacgg
      601 gatgcggtga cagcagagtg aaacggaata tacggctaca tgcaagatat gtcggacaac
      661 attccgctca tcgactcact cagtatgtgc agaacatggt atccggaatt ctttatccag
      721 agaaagttgc cgttggtgaa tcaataaatt ctgacgccat ttccggaaaa gtactttccg
      781 gacaaagaaa aaatatatct aaaaaaagac caggattgtt atgacacagc agcgttgtta
      841 ctacccccca ctgcggcgca tacggactcg ctacagtgta ggagccaaaa tatttctact
      901 gttctgggct gcaggattgg gaggcacgct gtacagcctg tacagcgtgc tggcgcactg
      961 acatctgtga gggaaggatg cgtttcgttc ttttctgtcc ctcaggaatt gtaccagcgc
     1021 agttcgcagc tctatcaacc ggttctgtcg gcaatgttac ctgcatcaga acagaagagg
     1081 agttacgcaa taaattgcgg cacaggccac agaatgtcgt gatatcggca gggcgacctg
     1141 ccgaatgcgc tgaaatgtgg ttccgatttt atcgggatca ctcattcgtg gtggtgttgt
     1201 gcgtcgcgcc cttttttctg ccaccggatg tgagtatttc tggcgttctc aaaaacctga
     1261 ggttactgaa accaggaatg tcagtagagc gtgtcatcag tattgcaaat acctctggtg
     1321 ggttttcagg gctgaaacat gcagaaattt tgccagttat ggacagttac tctgtcttta
     1381 tgaaagaggt gaataactgc acaaagacaa tcgtcatgtc agaacgcttc ccggaaaagc
     1441 aaaagaaggt gttgtcgttg cttcttgccg gacacagctg ggaatacagt gcgcagtttc
     1501 tcaaaacggg gataagacag atatggcttg ccgagcagtc gctaaaaaag cgttggggaa
     1561 taccggacag catgtcattg cgggaagccc tgctgctgag cagcaataat tttcacggtg
     1621 atggaaatgc attggaaaca acgaatgcca tgacattgcg tgaagacgga aataccaaca
     1681 ggtattccgt ggtggtaaat gcagggacgc aggctttatc tctgcataaa tataaataag
     1741 tgggaaattg aaattagtta ccagaatgga gaatttcttc atgaaaaaca gtaaagcatt
     1801 ttaccgcagc gcattagcaa cagctattgt tatggctctt tctgcaccag cattcgctgc
     1861 tgataacgcg gtatcaactg attcggttac gctgaataaa gataaaacga ctctggatca
     1921 agatgttgtt attaacaata cagcagacaa acagattaca gccgtaacaa ttaatgcggc
     1981 aaatgaagac ctgaatgtta cttttgctgg tcatgatatt actgctgaat caacggcaga
     2041 caaaaaattc gttgaaggtg taaaagttag tggtaacaaa aatgttgtga ttaatgctac
     2101 agactccacc atcacagctc aaggtgaagg cacctatgtc cggactgcaa tggtcatttc
     2161 ttcaactggc gatgttgttg ttaatggcgg taatttcgtt gcaaaaaatg aaaaaggtag
     2221 tgcgacaggg atatctctgg aagcgaccac gggaaataat ttaacgctca atggtacaac
     2281 cataaatgct caaggtaata agagttacag caacggctct acggcaattt ttgctcaaaa
     2341 gggtaatttg ttgcagggtt ttgacggtga tgcaaccgac aacatcaccc ttgctgactc
     2401 aaatattatt aatggcggga ttgaaacaat agttactgcc gggaataaga cgggaattca
     2461 tacagtcaac ctgaatatta aggatggctc agtaattggg gcggctaata ataaacaaac
     2521 aatttatgca tctgcttcgg cacaaggcgc aggttcagca acgcaaaatt taaatttgtc
     2581 tgttgctgat tcaaccatct actctgatgt cctggccctt tctgaaagcg agaattcagc
     2641 cggtaccaca acaaatgtaa atatgaacgt tgcccgctct tactgggaag gtaatgctta
     2701 tacgctcaat agcggcgata aagcgggtag tgatctggat ataaatcttt ccgatagttc
     2761 agtctggaaa ggcaaagttt caggggcagg agatgccagt gtatctctgc aaaacgggtc
     2821 tgtctggaat gttacgggtt cctcaactgt tgatgctctg gcagtaaaag acagtacggt
     2881 taatatcacg aaggctacag tcaatactgg cacgtttgct tctcaaaacg gcactctgat
     2941 tgttgatgcc tcttctgaaa acaccctgga tatcagcggt aaagcgagcg gtaacttgag
     3001 tgtttacagt gcgggttcat tggatcttat caatgaacaa acggcattta tttctaccgg
     3061 caaagacagc actctaaaag ccacaggcac aacggaaggt ggtctgtatc aatatgacct
     3121 gacacaggga gctgatggta acttttattt cgtaaaaaac acgcataaag catccaacgc
     3181 cagctccgtg attcaggcaa tggcagctgc tccggctaac gtcgctaatc tgcaggctga
     3241 cacgctctcc gcccgtcagg atgctgtccg tctgagcgaa aatgacaagg gtggcgtatg
     3301 gattcagtac tttggcggta aacagaaaca taccaccgcg ggaaatgcat cctatgacct
     3361 ggatgtaaat ggtgtaatgc tgggtggtga tacccgcttc atgactgaag atggtagctg
     3421 gctggccggt gtggcgatgt cttctgcgaa aggtgacatg actaccatgc agagcaaagg
     3481 tgacactgaa ggttacagct tccacgctta cctgagccgc cagtataaca acggtatctt
     3541 cattgatact gctgcacagt ttggtcacta cagcaacacg gcagatgttc gcctgatgaa
     3601 tggtggcggt accatcaaag ctgactttaa caccaatggt tttggtgcga tggttaaagg
     3661 cggttacaca tggaaagacg gtaatggcct gtttattcag ccatatgcca aactgtctgc
     3721 tctgactctg gaaggtgtgg attatcaact caacggcgtg gacgttcatt ctgacagcta
     3781 taactctgtg ctgggtgagg ccggtacgcg cgtgggttat gacttcgctg tgggcaacgc
     3841 gaccgttaaa ccttatctga atctggccgc actgaacgaa ttctctgatg gcaacaaagt
     3901 ccgtctgggt gatgagtctg tcaatgccag cattgacggt gcagcattcc gcgtgggtgc
     3961 aggtgtacag gctgatatca ccaaaaacat gggagcatat gctagccttg actacaccaa
     4021 aggtgacgac attgagaacc cgctacaggg tgtagttggt atcaatgtga cctggtaaga
     4081 ataaagcagt cggttcccct gtcgactgtt atacccccct gcaggcagca gtatgctgcc
     4141 tgcaggctat aaatatccag tattcatgat gctggctttt tgctgatatt taccctgaac
     4201 attcctgata cagactatta atacaatctt atctgcgaca gatttcattt tctggatata
     4261 ttatgaatga acaaatcaaa caggatatcg atctgattga gattttattt tatctgaaga
     4321 aaaagattcg tgttatcctt tttattatag ctatctgtat ggcaatggtg ctgttgtttc
     4381 tgtatatcaa taaagataat ataaaagtga cttacagtct aaaaataaac cagacaacac
     4441 ctggcatact tgttagctgt gatagcaata ataacttttc ctgtcagacc acaatgactg
     4501 aagatgttat tcagcgaatt actacatttt ttcagaccag cccatatgtc aagaacagag
     4561 aaataaagct ggaatggtca ggagataaga gagatttacc aactgctgaa gcggaaatat
     4621 ctcgcgtgca ggcgtctatc atcaaatggt atgcgtcaga ataccataat ggcaggcaag
     4681 ttctcgatga gatacaaacg ccttcagcaa ttaacagtga gctttataca aaaatgatat
     4741 acctgaccag gaactggtca ctgtatccga acggtgatgg ctgtgtcact ataagctcac
     4801 cagaaataaa taccctgctg ccatttgcct ggctctggga ttttttctaa gtattgtgat
     4861 ttctgtaatg ttttgccttg tcaaaaaaat ggtagatgaa taccaacaaa actctgggca
     4921 gtaataaaca gcattctaac agagagtgcc tgtataaaac tatagtgaca atgaccggga
     4981 agggagcccg tcttcaggaa tggattcctg tctttttcac tgtaaatatc ctggcattat
     5041 taatgtcagg atatttacag aagagcagca taaccatgac ggaacagggt taagtcaggt
     5101 tgtccttaac gctattcaca acctcgtatg ccgacttcag gtttatgtcc agactacata
     5161 tctctctcag caatcctcaa taatcagata caccgctttc accggaccat gaaccccgac
     5221 gactttgata agctcaatgt ccgccgttga actgggacca ctgatgatgt taatacatga
     5281 cggcattcgt tcaccggcct gcgctttctg atgcaatttt tctgcgagtt gcgctacacg
     5341 cggcaggatt gtgcttttac gcaggataaa aagagaagat tccgggagca ggctcaatga
     5401 acgcccgcgc ccggcggcgg aaaaaagaac cacgcctccc gattcggtta aaccatattc
     5461 agcatatacg acgcccactt tagcctgctc tgcttgtgat atgttctcaa tacctttcgc
     5521 cggatcccaa acaacggcat tgcattcctg ctgcaaacgt tcgctaatcc ccaattcctc
     5581 cagcctcgtg tcaccactaa tcacgaccga ctgatctccc agctctttac acagacgtat
     5641 tgcagcttct gccgccttcg cctcgctggt cagttcacag cgcgtcaaca taacatcgct
     5701 ggcaaactgg ataaacgcgt cacagcgctg ttgttgggta agttgggtaa gccgctcgtt
     5761 agcatagttg ttaagcggcg catcttctgc ttgcggttca agtcgcagcg ggcgacccag
     5821 tgcctgagca acgttattca aaaattcgct ccgattatcc attctttttc tcctgcgcct
     5881 gatgtttctt aaaccaacta cggaaactct ctccgtcagc ttcaggaaga tcgcgtgctt
     5941 ccatccagtc gctaatcgcg ccaaatttga gtggtgtttt gccgccattg ataaaccagc
     6001 ttgccgcatg agcaccagcc atcatcccaa ctttccacaa tcctggatga ctattggcat
     6061 aagcgaacat ttttatcgcc cgttgctctg cttttgcggt gatccctttt tcagccatca
     6121 cccgacgatg acgcaaaatc agttttgaca gcggaatacg caccggacac acgctgtcac
     6181 aagcggtgca taaagagcag gcgtagggta aatctttaaa atctttatag ccgccaagta
     6241 acggggaaat caccgcacca attggccctg gataaataga gccatatcca tgaccgccaa
     6301 tatggcgata tgccggacaa gtattcatac aagccccgca acgaatacag cgcagcacat
     6361 cccgaaattc agaggccagc acctcagaac gcccgttatc gacaataacc agatgaaact
     6421 cttcaggacc atcaacgtgc cccgcttcgc gcggtcctgt cagccaggtg ttgtatcccg
     6481 tcaaacgtgc accaacggca ctgcgcgcca gcatggtgat caatacatct acctctgcaa
     6541 acgtgggagc aatacgctcc attcccatca ctgcaatatg cgttttaggc agcgtggtac
     6601 acattcgcgc attaccttca ttggtcacca ggcataccga accggtctct gccaccgcga
     6661 aattacagcc ggtaatacct atttcagcac tgaggaaatc ttcgcggatt ttttgccgaa
     6721 tgaataaggt catcgcttca ggcgtttccg gcccctcata gcccagacgt tcgtgtagca
     6781 ctcgacggat ctgatggcga tctttatgaa ttgccgggac cacaacatga gagggcggat
     6841 cttgatccag ctggagaatg tattcaccca gatcggtttc aatcacctga atcccggcat
     6901 cctgcaacac atgattgaca ccaatctctt cggtcaccat cgatttagat ttcactacct
     6961 tccgggcatt tttgcgttgg gcaacctgta aaatgtagcg ggtggcgtct tctttggttt
     7021 ttgcaaaata gacgtgaccg ccgttttgtg tcactttttc tgagagctgg tacagataag
     7081 cgtcgagatt actcagaaca tgatcacgta tctgggcggc ccgatcgcgc cactcctccc
     7141 agtgccccaa ttcatcgacc attttttgcc gatttgcccc aatacgctgc tgcgcgtttg
     7201 ccaccgcttt acgcatgatc ggatcttcaa tttgctgacg gatgcgtgtc ttaaaatctg
     7261 tattactggt tttgatcgac atctttatat cctcagcggc tcatcagcac ttcagcaata
     7321 tgcatcactt tgactttctg cccttcccgt tgtaatcgtc cactgatgtt cagcaggcaa
     7381 ctcacgtcag caccaattaa atactcaggg cggacttcca tcaggtgcgc aaccttttct
     7441 ttcaccatct cgccggatat ttcggccatt ttgaccgaga acgtgccgcc aaatccgcag
     7501 caggtatcct gttcagcaaa ggtcaacagc tccagtccac gcacattttt cagcagcgta
     7561 agtggttcgt ccttcacccc cagcttacgg gccaggctac aagatgggtg atacaccgct
     7621 ctcccttgca aactggcacc tacatcgact acccctaatg tattaacaat aaaagaggtg
     7681 agatcctgca tacgcgcggc aaccttttcg gcacgtgatg cccattcagg ttcatccgcc
     7741 agatacgtcg ggtaactttt tacggcatag gtgcaagagc cagccggtga aataatggga
     7801 tcgtcgttat cctccagtgc ggcgatcaga tttttcatcc ctggaatcgc ttctttgata
     7861 taaccactat tgatcgcagg ctgaccgcag catccctgtt tctccgggaa atttacgcga
     7921 cagccgagtt tttccagtaa cagcacggag tctcgtgcca ttcttgattt cagggcgtca
     7981 ccaatacagg tgacaaagaa attgacattc acaactaata ctccattact tcatgcccat
     8041 ttatgcccga tggttccccc cttccggggg atcaatcatc aataaatatg gcgttctttt
     8101 ttaattggct agcaacatac cacccctcat ttgtatgaca aatgataatc tttcatctaa
     8161 acttcaaatg caataactaa ttttatttct ttcggcatcg ctcacatttt atagggaaat
     8221 cgacgatcat ttttggcata agaaatccat ttaaaaaggc tacatccctt atacctattc
     8281 agagctactt attcaggggt tattgagata tacactgatg gtgtacaaaa aaaatcaccg
     8341 acaataatca cgtatcaagt aatagtcggg attttattta attgaagaaa tttagtttgc
     8401 atttttattt aaaaactgag ctagagtcca tcaaaaaaat atataaaacc caaaaccacc
     8461 gttggaaata gaaatagttg aaaggctcac aactcccgga atgcggatta tctttgccct
     8521 gatttctggc ggttttattc agggtgcaag ctgtctcacc ctttcccgat attctcccgg
     8581 agtacaacca aactcgcgga caaacgcctt gtgaaaagat gattcgctgg catagcctac
     8641 tgactcagcg atcaccacaa cagggagcat ttcccgggaa aacatctggg ccgctatttg
     8701 tagacgcaac tttgttaata cagccagcgg cgtggttccg gaaacatcac ggaaaagctg
     8761 ggcaaaactt gcccgggaca tgtgggcgat gctggccagc gattcgacgg tccaggcgtg
     8821 tccaggcatt tccatcattt gctgtattac cgcaccaaga cgtggatgca gaagcaaact
     8881 gagaatgttt ttctctgtat taacctgtgc aatccattca cgcaccgcga gggtaaagaa
     8941 tgtagcgcag atctggctac acagagcatc cactcccggc attgccgatc tggattcctg
     9001 ttgtaaaaac gggatcgcct ccctcagcca gttatattcc acgctgtggt taaccggtgc
     9061 tacaaacagc gtttccggca aagacgttaa aaaataacgc gccgaatgtt gcagccgaag
     9121 agttccacag acaatacagg tcgattcatt atcgacatga ctcaggcgat gagctgaatt
     9181 ttgtggtagc aggaccacat ttcccgggcg taatgtaaaa atctctcccg tcggcatttc
     9241 cagcttcgcc gctccttgcg ttaacgcatg ccaacgaata accgacaatt ccccggcacc
     9301 atgtggaagt tgccagtcac ttcctaacac gcaattctta tcgatcgttc cttgtggagc
     9361 gttaagcatc aacaaacggc taagagcatc catatcagac tcctgagtgg ttttttgatt
     9421 acaaacacca acaatcttag tcaataat
//
LOCUS       DADSYM010000164         9351 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2415
            SAMN08039579-rid11723173.denovo.166, whole genome shotgun sequence.
ACCESSION   DADSYM010000164 DADSYM010000000
VERSION     DADSYM010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039579
            Sequence Read Archive: SRR6362533
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9351)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9351)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980162.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 03:39:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,484
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 4,347
            CDSs (with protein)               :: 4,347
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 43 of 85
            Pseudo Genes (incomplete)         :: 37 of 85
            Pseudo Genes (internal stop)      :: 19 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9351
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039579-rid11723173.denovo.166"
                     /strain="LGE2415"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8302 N 95.9628 W"
                     /collection_date="2015-08-24"
     gene            316..1614
                     /gene="kgtP"
                     /locus_tag="J1Y83_003778"
     CDS             316..1614
                     /gene="kgtP"
                     /locus_tag="J1Y83_003778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoglutarate permease"
                     /protein_id="HBA7631883.1"
                     /translation="MAESTVTADSKLTSSDTRRRIWAIVGASSGNLVEWFDFYVYSFC
                     SLYFAHIFFPSGNTTTQLLQTAGVFAAGFLMRPIGGWLFGRIADKHGRKKSMLLSVCM
                     MCFGSLVIACLPGYETIGTWAPALLLLARLFQGLSVGGEYGTSATYMSEVAVEGRKGF
                     YASFQYVTLIGGQLLALLVVVVLQHTMEDAVLREWGWRIPFALGAVLAVVALWLRRQL
                     DETSQQETRALKEAGSLKGLWRNRRAFIMVLGFTAAGSLCFYTFTTYMQKYLVNTAGM
                     HANVASGIMTAALFVFMLIQPLIGALSDKIGRRTSMLCFGSLATIFTVPILSALQNVS
                     SPYAAFGLVMCALLIVSFYTSISGILKAEMFPAQVRALGVGLSYAVANAIFGGSAEYV
                     ALSLKSIGMETAFFWYVTLMAVVAFLVSLMLHRKGKGMRL"
     gene            complement(1611..1955)
                     /locus_tag="J1Y83_003779"
     CDS             complement(1611..1955)
                     /locus_tag="J1Y83_003779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708437.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiM family lipoprotein"
                     /protein_id="HBA7631884.1"
                     /translation="MFFGGHVLRILFVCSLLLLSGCSHMANDSWSGQDKAQHFIASAM
                     LSAAGNEYSQHQGMSRDRSAMFGLMFSVSLGASKELWDSRPEGSGWSWKDLAWDVAGA
                     STGYTVWQLTRH"
     gene            complement(1980..3335)
                     /gene="pssA"
                     /locus_tag="J1Y83_003780"
     CDS             complement(1980..3335)
                     /gene="pssA"
                     /locus_tag="J1Y83_003780"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HBA7631885.1"
                     /translation="MLSKFKRNKHQQHLAQLPKISQSVDDVDFFYAPADFRETLLEKI
                     ASAKQRICIVALYLEQDDGGKGILNALYEAKRQRPELDVRVLVDWHRAQRGRIGAAAS
                     NTNADWYCRMAQENPGVDVPVYGVPINTREALGVLHFKGFIIDDSVLYSGASLNDVYL
                     HQHDKYRYDRYHLIRNRKMSDIMFEWVTQNIMNGRGVNRLDDVNRPKSPEIKNDIRLF
                     RQELRDAAYHFQGDADNDQLSVTPLVGLGKSSLLNKTIFHLMPCAEQKLTICTPYFNL
                     PAILVRNIIQLLREGKKVEIIVGDKTANDFYIPEDEPFKIIGALPYLYEINLRRFLSR
                     LQYYVNTDQLVVRLWKDDDNTYHLKGMWVDDKWMLITGNNLNPRAWRLDLENAILIHD
                     PQLELAPQREKELDLIREHTTIVKHYRDLQSIADYPVKVRKLIRRLRRIRIDRLISRI
                     L"
     gene            complement(3449..6109)
                     /gene="pat"
                     /locus_tag="J1Y83_003781"
     CDS             complement(3449..6109)
                     /gene="pat"
                     /locus_tag="J1Y83_003781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein lysine acetyltransferase"
                     /protein_id="HBA7631886.1"
                     /translation="MSQRGLEALLRPKSIAVIGASMKPNRAGYLMMRNLLAGGFNGPV
                     LPVTPAWKAVLGVLAWPDIASLPFTPDLAVLCTNASRNLALLEELGEKGCKTCIILSA
                     PASQHEDLRACALRHNMRLLGPNSLGLLAPWQGLNASFSPVPIKRGKLAFISQSAAVS
                     NTILDWAQQREMGFSYFIALGDSLDIDVDELLDYLARDSKTSAILLYLEQLSDARRFV
                     SAARSASRNKPILVIKSGRSPAAQRLLNTTAGMDPAWDAAIQRAGLLRVQDTHELFSA
                     VETLSHMRPLRGDRLMIISNGAAPAALALDALWSRNGKLATLSEETCQKLRDALPGHV
                     AISNPLDLRDDASSEHYVKTLDILLHSQDFDALMVIHSPSAAAPATESAQVLIEAVKH
                     HPRSKYVSLLTNWCGEHSSQEARRLFSEAGLPTYRTPEGTITAFMHMVEYRRNQKQLR
                     ETPALPSNLTSNTAEAHLLLQQAIAQGATSLDTHEVQPILQAYGMNTLPTWIASDSTE
                     AVHIAEQIGYPVALKLRSPDIPHKSEVQGVMLYLRTANEVQQAANAIFDRVKMAWPQA
                     RVHGLLVQSMANRAGAQELRVVVEHDPVFGPLIMLGEGGVEWRPEDQAVVALPPLNMN
                     LARYLVIQGIKSKKIRARSALRPLDVAGLSQLLVQVSNLIVDCPEIQRLDIHPLLASG
                     SEFTALDVTLDIAPFEGDNESRLAVRPYPHQLEEWVELKNGERCLFRPILPEDEPQLQ
                     QFISRVTKEDLYYRYFSEINEFTHEDLANMTQIDYDREMAFVAVRRIDQTEEILGVTR
                     AISDPDNIDAEFAVLVRSDLKGLGLGRRLMEKLITYTRDHGLQRLNGITMPNNRGMVA
                     LARKLGFNVDIQLEEGIVGLTLSLAQRKES"
     gene            complement(6141..6839)
                     /locus_tag="J1Y83_003782"
     CDS             complement(6141..6839)
                     /locus_tag="J1Y83_003782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311476.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DTW domain-containing protein"
                     /protein_id="HBA7631887.1"
                     /translation="MTENAVLQLRAERIARATRPFLARGNRVRRCQRCLLPEKLCLCS
                     TITPAQAKSRFCLLMFDTEPMKPSNTGRLIADILPDTVAFQWSRTEPSQDLLDLVQNP
                     DYQPMVVFPASYADEQREVIFTPPADKPPLFIMLDGTWPEARKMFRKSPYLDNLPVIS
                     VDLSRLSAYRLREAQAEGQYCTAEVAIALLDMAGDTRAATGLGEHFTRFKTRYLAGKT
                     QHPGSITAEQLESV"
     gene            complement(6908..7327)
                     /gene="trxC"
                     /locus_tag="J1Y83_003783"
     CDS             complement(6908..7327)
                     /gene="trxC"
                     /locus_tag="J1Y83_003783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxC"
                     /protein_id="HBA7631888.1"
                     /translation="MNTVCTHCQAINRIPDDRIEDAAKCGRCGHDLFDGEVINATGET
                     LDKLLKDDLPVVIDFWAPWCGPCRNFAPIFEDVAQERSGKVRFVKVNTEAERELSSRF
                     GIRSIPTIMIFKNGQVVDMLNGAVPKAPFDSWLNESL"
     gene            7534..8571
                     /locus_tag="J1Y83_003784"
     CDS             7534..8571
                     /locus_tag="J1Y83_003784"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA/rRNA methyltransferase"
                     /protein_id="HBA7631889.1"
                     /translation="MNDEMKGKSGKVKVMYVRSDDDSDKRTHNPRTGKGGGRPGKSRA
                     DGGRRPARDDKQSQHRDRKWEDSPWRTVSRAPGDETPEKADHGGISGKSFIDPEVLRR
                     QRAEETRVYGENACQALFQSRPEAIVRAWFIQSVTPRFKEALRWMAANRKAYHVVDEA
                     ELTKASGTEHHGGVCFLIKKRNGTTVQQWVSQAGAQDCVLALENESNPHNVGGMMRSC
                     AHFGVKGVVVQDAALLESGAAIRTAEGGAEHVQPITGDNIVNVLDDFRQAGYTVVTTS
                     SEQGKPLFKTSLPAKMVLVLGQEYEGLPDAARDPNDLRVKIDGTGNVAGLNISVATGV
                     LLGEWWRQNKA"
     gene            complement(8619..9308)
                     /gene="ung"
                     /locus_tag="J1Y83_003785"
     CDS             complement(8619..9308)
                     /gene="ung"
                     /locus_tag="J1Y83_003785"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HBA7631890.1"
                     /translation="MANELTWHDVLAEEKQQPYFLNTLQTVASERQSGVTIYPPQKDV
                     FNAFRFTELGDVKVVILGQDPYHGPGQAHGLAFSVRPGIATPPSLLNMYKELENTIPG
                     FTRPNHGYLESWARQGVLLLNTVLTVRAGQAHSHASLGWETFTDKVISLINQHREGVV
                     FLLWGSHAQKKGAIIDKQRHHVLKAPHPSPLSAHRGFFGCNHFVLANQWLEQRGETPI
                     DWMPVLPAESE"
BASE COUNT         2253 a         2388 c         2447 g         2263 t
ORIGIN      
        1 agcgaaaggc ccagtcggaa gactgggcct ttttgcatat gtgcggaata aatgccggat
       61 gcgacgctgg cgcgtcttat actcccacat aagccagatt caacagcgaa tacgtcttcc
      121 ccaaattgcc cacttccata cttcctcctt accagaaatc tatccttaag ctccttaata
      181 accattttcc tgctaactaa attcatggtt aaggttgcat aatgatatgc aacaaatgta
      241 taacattttt tcttaccaaa aaataaacaa aagcgaccga caaaagcatt ggattacggc
      301 aggagacata atggcatggc tgaaagtact gtaacggcag acagcaaact gacaagtagt
      361 gatactcgtc gccgcatttg ggcaattgtt ggggcctctt caggtaatct ggtcgagtgg
      421 ttcgatttct atgtctactc gttctgttca ctctacttcg cccacatctt cttcccttcc
      481 gggaacacga cgactcaact actgcaaaca gcaggtgttt ttgctgcagg atttctgatg
      541 cgcccaatag gcggttggct gtttggccgc atagccgata aacatggtcg caaaaaatcg
      601 atgctgttat cggtgtgtat gatgtgtttc ggctcactgg ttatcgcctg tttgccaggc
      661 tacgaaacaa tcggtacctg ggctccggca ttattgcttc tcgcgcgttt attccaggga
      721 ttatccgttg gcggagaata tggcaccagc gccacctata tgagtgaagt tgccgttgaa
      781 gggcgcaaag gtttttacgc atcatttcag tatgtgacgt tgatcggcgg gcaactgcta
      841 gctctactgg ttgtcgtggt tttacaacac accatggaag acgctgtact cagagagtgg
      901 ggatggcgta ttcctttcgc gttaggggct gtgttagctg ttgtggcgtt gtggttacgt
      961 cgtcagttag atgaaacttc acaacaagaa acacgcgctt taaaagaagc tggatctctg
     1021 aaaggattat ggcgcaatcg ccgtgcattc atcatggttc tcggttttac cgctgcgggc
     1081 tccctttgtt tctatacctt caccacttat atgcagaagt atctggtaaa tactgcggga
     1141 atgcatgcca acgtggcgag tggcattatg actgccgcat tgtttgtatt catgcttatt
     1201 caaccactta ttggcgcgct gtcggataag attggtcgcc gtacctcaat gttatgtttc
     1261 ggttctctgg caaccatttt taccgttcct attctctcag cattgcagaa cgtttcctcg
     1321 ccttatgccg cttttggtct ggtgatgtgc gccctgctga tagtgagttt ttatacttca
     1381 atcagtggaa tactgaaggc tgagatgttc ccggcacagg ttcgcgcatt aggcgttggt
     1441 ctgtcatatg cggtcgctaa tgctatattt ggtggttcgg cggagtacgt agcgttgtcg
     1501 ctgaaatcaa taggaatgga aacagccttc ttctggtatg tgaccttgat ggccgtggtg
     1561 gcgtttctgg tttctttgat gctacatcgc aaagggaagg ggatgcgtct ttagtgacgg
     1621 gtcagttgcc agacggtata gccggtgctt gcaccggcga catcccaggc caaatccttc
     1681 cagctccagc cgctcccttc ggggcggcta tcccaaagct cttttgacgc ccccaaactg
     1741 acagagaaca tcaatccaaa catggcactg cgatcccggc tcatcccctg atgctgtgaa
     1801 tattcatttc cggcggcgga aagcatcgcc gaggcgataa agtgttgagc tttatcctgc
     1861 ccgctccagc tatcgttagc catatggctg catccagaaa gtaacaatag cgaacagaca
     1921 aaaagaatac gcaaaacgtg gcctccaaaa aacaaacccc gtgcaggacg gggttgtgat
     1981 tacaggatgc ggctaattaa tcggtcgatg cggatacggc gcaaacggcg gatgagttta
     2041 cgaaccttca ccggataatc ggcaatactt tgcagatcgc ggtagtgctt aacgatggtg
     2101 gtatgctcgc ggatcagatc cagttctttc tctcgctgtg gcgcaagctc cagttgcgga
     2161 tcgtggatca aaatggcgtt ttccagatcc agacgccagg cgcgcgggtt caggttatta
     2221 ccggtgatca acatccactt atcatcaacc cacatccctt tcaggtgata ggtgttgtcg
     2281 tcatctttcc ataaccgaac tactaactgg tcagtgttga cgtaatactg taaacgactc
     2341 aggaaacgac gcagattgat ctcatagaga taaggcaatg cgccaattat cttgaaaggt
     2401 tcatcttccg gaatgtagaa gtcattcgcg gttttatcac caacaataat ttcgaccttt
     2461 ttcccttcgc gcagcaactg gatgatattg cgcacaagga ttgctggtag gttgaagtaa
     2521 ggtgtgcaga tggttagttt ctgctcggca caaggcataa gatggaaaat ggtcttgttc
     2581 aacagactcg atttccccag ccccactagc ggcgttacgg aaagctgatc gttgtcggca
     2641 tcgccctgga aatgataagc ggcatcacgc agctcctgtc ggaacaggcg aatgtcgttc
     2701 ttgatttccg ggctttttgg ccgattaaca tcatccagac gattaacgcc gcggccattc
     2761 ataatattct gtgtaaccca ttcaaacata atgtctgaca tcttacggtt acggatcaga
     2821 tgataacggt cgtagcgata tttatcgtgc tgatgcaggt aaacatcgtt caggctggca
     2881 ccgctataaa gtacgctatc gtcgatgata aagcctttaa agtgcagaac accaagggct
     2941 tcacgggtat tgattggcac gccataaacc ggaacatcta cgcccggatt ttcctgcgcc
     3001 atgcggcagt accagtcagc gttagtgtta gatgctgcag cgccaatgcg tccacgttgt
     3061 gcacgatgcc agtcgaccag cacccgcaca tccagttccg gacgctgcct tttagcctca
     3121 tacaacgcgt tcagaatgcc tttgccaccg tcatcctgtt cgagatacag ggcaacaatg
     3181 caaatgcgct gcttcgcgct ggctattttt tccagcaacg tctcccggaa gtcggcggga
     3241 gcgtaaaaga aatcgacatc atcaactgat tgagaaatct tgggtagttg ggcaaggtgt
     3301 tgttgatgtt tattacgctt aaattttgac aacatcacag tgcatttctt ctctgttcat
     3361 tgaagggtcc tctgtgcaat gcagacgaca taagcgggca ataataacac cagtcccgat
     3421 taagtggtca acatttccag taccttactc atgattcctt gcgctgggca agacttagcg
     3481 taagcccaac gatcccctct tcgagctgga tatcaacgtt aaacccgagc ttgcgggcca
     3541 gcgccaccat gccacgattg tttggcatcg taataccatt cagacgttgt agtccgtgat
     3601 ctcgcgtata ggtaatcaac ttttccatta agcgtcgacc taagcctaac cctttgagat
     3661 ccgagcgaac cagcacggca aattcggcat cgatgttatc agggtcggag atcgcacgtg
     3721 tgacgccgag gatctcttcc gtttgatcaa tacgtcgtac cgctacaaat gccatttccc
     3781 gatcgtagtc gatctgcgtc atgttggcta aatcttcatg ggtaaattcg ttgatctcgc
     3841 taaagtagcg gtaataaaga tcttctttgg tgactcgcga aatgaattgc tgaagctgtg
     3901 gctcatcttc tggcaaaatc gggcggaaca agcagcgttc accgtttttc aattctaccc
     3961 attcttccag ctgatgcgga taagggcgca cagccagccg gctctcgtta tcaccttcaa
     4021 acggcgcgat atccagcgtg acatccagcg cggtaaattc actgccagaa gccagcaaag
     4081 gatgaatatc cagacgctga atttccgggc aatcgacaat caagttggaa acctgcacca
     4141 gaagctggct caagcctgca acatccaatg ggcgtagcgc actgcgtgca cgaatctttt
     4201 tacttttgat cccctgaata accagatagc gggccaggtt catgttcagc ggcggcagtg
     4261 cgacgacggc ttgatcttca ggacgccact ccacaccgcc ttcacccagc atgattaacg
     4321 gcccgaaaac cggatcgtgc tcaaccacaa cccgcaactc ctgagcgcca gcacggttag
     4381 ccatactttg caccaacagg ccgtggaccc gcgcctgcgg ccaggccatt tttacgcgat
     4441 cgaaaatagc gtttgccgct tgctggactt cattggctgt acgtaggtag agcatgacgc
     4501 cctgaacttc cgatttatgt ggaatatccg gcgagcgcaa tttcagtgcc accggatagc
     4561 caatctgttc ggcaatatgt accgcttcgg tgctatcact ggcaatccag gtagggagag
     4621 tattcatacc atacgcttgc aggatgggct gaacttcatg ggtatcgagc gacgtagccc
     4681 cttgggcaat cgcctgttgc aacagaagat gcgcttctgc ggtattggag gtcagattgc
     4741 tgggcaacgc cggcgtttcg cgtagttgct tctgattacg ccggtactcg accatatgca
     4801 taaaagcagt gatggttcct tccggagtac ggtaggtcgg cagcccggct tcgctgaata
     4861 aacgtcgtgc ctcttgcgag gagtgctcgc cgcaccagtt cgtcagcaaa gagacatatt
     4921 tgctgcgggg atgatgcttt accgcttcaa ttaatacttg cgcgctttct gttgcgggag
     4981 cagcggcgct gggcgaatga ataaccatca gcgcatcaaa atcctggctg tggagcagaa
     5041 tgtccagcgt tttgacataa tgctcgctgc tggcgtcatc gcgcagatcg agcgggttag
     5101 agattgccac atgccctggc agtgcatcgc gcagtttctg gcaggtttct tcgcttagcg
     5161 ttgccagctt gccattgcgt gaccataagg catccagcgc cagcgcggca ggcgcagcac
     5221 cgttgctgat aatcatcagc cggtcgccac gcagcgggcg catatggctg agggtttcca
     5281 ccgctgaaaa cagctcgtga gtatcctgta cccgcaacaa accggcacgc tgaatagccg
     5341 catcccacgc cggatccatt cctgccgtgg tgttcaacag acgttgagcc gccgggctac
     5401 gtccgctttt aatcaccaga atcggtttgt tgcgagaggc actacgggcc gccgacacaa
     5461 agcgtcgcgc gtcgcttaac tgttcgagat agagcaggat ggcgctggtt ttactgtcgc
     5521 gggccagata gtcgagcaat tcatcaacgt cgatatccag gctgtcgccg agcgcaataa
     5581 agtaggaaaa gcccatctca cgctgttgcg cccagtcgag gatggtattg gagacggcag
     5641 ccgattgcga aataaacgcc agcttgccgc gtttaatcgg cacaggcgaa aagctggcat
     5701 tcagaccttg ccagggagcc agtaacccca gactgtttgg tcctagcagg cgcatgttat
     5761 ggcgcagggc gcaggcgcgg agatcttcat gttgcgatgc cggggcggaa agaataatgc
     5821 aggttttaca gcctttctcg ccgagctctt ccagaagagc aagattacgg ctggcattgg
     5881 tacataaaac cgcaaggtcg ggtgtaaagg gcaagctggc aatatccggc caggccaaca
     5941 cacccaacac cgctttccag gctggcgtca ccgggagtac cggtccgtta aagcctcccg
     6001 ccagcaggtt acgcatcatc aggtaacctg cgcgattggg tttcatcgat gcgccaatta
     6061 ccgcaatcga ttttggtcgc agtagtgctt ccagtcctcg ctgactcata ccggtctccc
     6121 gcagaagtga ccgaatgatt ttaaacgctt tctaactgtt ctgctgtgat gcttcccgga
     6181 tgttgcgttt ttcctgccag atagcgtgtt ttaaagcggg taaaatgctc gcctaaccct
     6241 gtcgccgccc tggtatcgcc ggccatatct aacagtgcga tggctacctc ggcagtacaa
     6301 tattggcctt cagcctgggc ttcacgcagg cgataggcag aaagccgaga aagatcgacg
     6361 gaaatcacgg gaagattatc cagatacgga cttttacgaa acatcttgcg agcttctggc
     6421 caggttccat cgagcatgat aaatagcgga ggcttatcgg caggtggtgt gaagatcact
     6481 tcccgttgct catcagcata cgaagcggga aagaccacca ttggctgata gtccgggttt
     6541 tgcaccagat ccagcaaatc ttgcgagggt tcggtacgcg accattgaaa cgcaacggta
     6601 tcaggcaaaa tatcagcaat cagacgcccg gtattactgg gcttcattgg ttcggtgtcg
     6661 aacatcagca aacagaagcg actttttgct tgtgctgggg taattgtcga acagagacat
     6721 aatttctctg gcaaaagaca gcgttggcag cgacgaacgc gattaccgcg ggcaagaaaa
     6781 ggacgtgttg cacgcgcaat acgctcggcg cgtaactgga gaacagcgtt ttcggtcata
     6841 agggagcgtc gaaaaaacgc cattgtcgca gaggagaaaa cggggcacaa gatgtgcccc
     6901 ggtaagatta aagagattcg ttcagccagc tgtcgaacgg cgctttcggt actgcgccat
     6961 taagcatgtc gacaacctga ccatttttga aaatcatgat cgtcggtata ctacgaattc
     7021 caaagcgact gctcaattca cgttcagctt cggtattcac tttcacaaag cgcactttac
     7081 cgctacgctc ttgcgcgaca tcttcaaaaa ttggtgcgaa attacggcag gggccgcacc
     7141 acggtgccca gaagtcgatc accacaggta gatcatcttt cagcaatttg tcgagcgttt
     7201 caccggtcgc attaatcacc tctccgtcaa acaagtcgtg accgcagcgt ccgcattttg
     7261 ccgcatcttc gatccgatcg tcgggaatgc gattgatggc ctgacaatgg gtacaaacgg
     7321 tattcataac taacctcggg atgagtaagg agacttatcg gcaacgacgc cggtatgttt
     7381 ctaatatgtt acatattatc ggggacattg ttccagacaa caatctgttt tattcaatgg
     7441 gtatgataga cacaggcttt ggaacgaaat catggcgaac cgcgcggact tcgggtaatc
     7501 tgcgcgcttc gcgcagcgct ggtggagaaa agcatgaacg atgaaatgaa aggtaaaagc
     7561 ggcaaggtca aagtgatgta tgtccgcagt gatgatgatt ctgataaacg tacccacaac
     7621 ccgcgtaccg ggaaaggggg cgggcgtcca ggaaaatctc gtgctgacgg tggccgtcgc
     7681 cccgcccgcg atgacaaaca gagtcagcac cgtgaccgca agtgggaaga ttcgccgtgg
     7741 cgcacggttt cccgcgcacc gggtgatgag acgccggaaa aggccgatca cggtggcatc
     7801 agtggtaaaa gttttattga tccggaagtt ttgcgtcgtc agcgtgcgga agaaacccgc
     7861 gtctacggcg aaaacgcctg tcaggcactg ttccagagcc gtccggaagc gattgttcgc
     7921 gcctggttta tccagagcgt aacgccacgt tttaaagaag ccttgcgctg gatggcggca
     7981 aaccgcaaag cgtaccatgt ggtggatgaa gcggaactga caaaagcgtc aggcacggaa
     8041 catcacggcg gcgtttgctt cctgatcaaa aagcgtaacg gtacaaccgt gcagcagtgg
     8101 gtaagccagg caggcgcgca ggattgtgta ctggccctgg aaaacgaatc taacccgcat
     8161 aacgtgggcg gcatgatgcg tagctgtgcg cacttcggcg tgaaaggtgt tgtggtgcag
     8221 gatgcagcac tgctggagtc tggggcggct atccgtaccg cagaaggcgg cgcagagcac
     8281 gttcagccga ttactggcga caacattgtt aacgtgctgg atgatttccg tcaggcgggt
     8341 tacaccgtag tgacaacttc cagcgagcag ggtaaaccgc tgttcaaaac cagtctgcca
     8401 gcgaaaatgg tactggtgct gggtcaggaa tatgaagggt taccggatgc cgcacgcgat
     8461 ccgaacgatc tgcgcgtgaa gattgatggt actggcaacg ttgccgggct gaatatctct
     8521 gtcgcaaccg gcgttcttct tggcgaatgg tggcgtcaga ataaagcctg ataaatcagc
     8581 cgggtggcaa ctctgccatc cggcattttc ccgcaaattt actcactctc tgccggtaat
     8641 actggcatcc agtcaatcgg cgtctcgcca cgttgttcca gccactgatt tgccagcaca
     8701 aaatggttgc agccaaagaa tccacgatgc gccgaaagcg gcgacggatg cggtgctttc
     8761 agtacatgat ggcgttgctt atctataatc gcccctttct tttgcgcatg tgatccccac
     8821 aacaaaaaca ccacgccttc gcgatgctga tttatgaggc tgatgacttt atcagtaaac
     8881 gtctcccagc cgaggctggc gtgggaatgc gcctgaccag cgcgaaccgt caagacagta
     8941 ttgagcagca gaacaccctg acgcgcccag ctttcaagat aaccatgatt agggcgggtg
     9001 aaaccgggaa tagtattttc cagctcttta tacatattca gcagtgacgg tggtgtcgca
     9061 atgccggggc gaacggaaaa tgccagacca tgcgcctgtc ctggtccgtg ataaggatcc
     9121 tgaccgagaa tcaccacttt aacgtcaccc aactccgtaa agcggaacgc gttaaagaca
     9181 tctttttgtg gtggatagat agtgacgccg gactgccgct cgctggcgac ggtctgaagg
     9241 gtattaagaa aatagggttg ctgcttctct tcagccagca catcatgcca ggttaattcg
     9301 ttagccatct cgctctcctg cgaatcttta atccgcctag cttaactgct t
//
LOCUS       DADSYM010000165         9289 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2415
            SAMN08039579-rid11723173.denovo.167, whole genome shotgun sequence.
ACCESSION   DADSYM010000165 DADSYM010000000
VERSION     DADSYM010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039579
            Sequence Read Archive: SRR6362533
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9289)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9289)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980162.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 03:39:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,484
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 4,347
            CDSs (with protein)               :: 4,347
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 43 of 85
            Pseudo Genes (incomplete)         :: 37 of 85
            Pseudo Genes (internal stop)      :: 19 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9289
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039579-rid11723173.denovo.167"
                     /strain="LGE2415"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8302 N 95.9628 W"
                     /collection_date="2015-08-24"
     gene            324..1664
                     /locus_tag="J1Y83_003786"
     CDS             324..1664
                     /locus_tag="J1Y83_003786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter"
                     /protein_id="HBA7631891.1"
                     /translation="MLFTIQLIIILICLFYGARKGGIALGLLGGIGLVILVFVFHLQP
                     GKPPVDVMLVIIAVVAASATLQASGGLDVMLQIAEKLLRRNPKYVSIVAPFVTCTLTI
                     LCGTGHVVYTILPIIYDVAIKNNIRPERPMAASSIGAQMGIIASPVSVAVVSLVAMLG
                     NVTFDGRHLEFLDLLAITIPSTLIGILAIGIFSWFRGKDLDKDEEFQKFISVPENREY
                     VYGDTATLLDKKLPKSNWLAMWIFLGAIAVVALLGADSDLRPSFGGKPLSMVLVIQMF
                     MLLTGALIIILTKTNPASISKNEVFRSGMIAIVAVYGIAWMAETMFGAHMSEIQGVLG
                     EMVKEYPWAYAIVLLLVSKFVNSQAAALAAIVPVALAIGVDPAYIVASAPACYGYYIL
                     PTYPSDLAAIQFDRSGTTHIGRFVINHSFILPGLIGVSVSCVFGWIFAAMYGFL"
     gene            1742..3388
                     /locus_tag="J1Y83_003787"
     CDS             1742..3388
                     /locus_tag="J1Y83_003787"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fumarate hydratase"
                     /protein_id="HBA7631892.1"
                     /translation="MSNKPFIYQAPFPMGKDNTEYYLLTSDYVSVADFDGETILKVEP
                     EALTLLAQQAFHDASFMLRPAHQKQVAAILHDPEASENDKYVALQFLRNSEIAAKGVL
                     PTCQDTGTAIIVGKKGQRVWTGGGDEEALSKGVYNTYIEDNLRYSQNAPLDMYKEVNT
                     GTNLPAQIDLYAVDGDEYKFLCVAKGGGSANKTYLYQETKALLTPGKLKNFLVEKMRT
                     LGTAACPPYHIAFVIGGTSAETNLKTVKLASAHYYDELPTEGNEHGQAFRDIQLEQEL
                     LEEAQKLGLGAQFGGKYFAHDIRVIRLPRHGASCPVGMGVSCSADRNIKAKINREGIW
                     IEKLEHNPGQYIPEELRQAGEGEAVKVDLNRPMKEILAQLSQYPVSTRLSLTGTIIVG
                     RDIAHAKLKELIDAGKELPQYIKDHPIYYAGPAKTPAGYPSGSLGPTTAGRMDSYVDL
                     LQSHGGSMIMLAKGNRSQQVTDACHKHGGFYLGSIGGPAAVLAQQSIKHLECVAYPEL
                     GMEAIWKIEVEDFPAFILVDDKGNDFFQQIVNKQCANCTK"
     gene            3511..4161
                     /locus_tag="J1Y83_003788"
     CDS             3511..4161
                     /locus_tag="J1Y83_003788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000198766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2238 domain-containing protein"
                     /protein_id="HBA7631893.1"
                     /translation="MTRTLKPLILNTGALALTLILIYTGISAHDKLTWLMEVTPVIIV
                     VPLLLATAKRYPLTPLLYTLIFFHAIILMVGGQYTYAKVPVGFEVQEWLGLSRNPYDK
                     LGHFFQGLVPALVAREILVRGMYVRGRKMVAFLVCCVALAISAMYELIEWWAALAMGQ
                     GADDFLGTQGDPWDTQSDMFCALLGALTTVIFLARFHCRQLRRFGLITRAPEAITP"
     gene            complement(4158..5513)
                     /gene="melA"
                     /locus_tag="J1Y83_003789"
     CDS             complement(4158..5513)
                     /gene="melA"
                     /locus_tag="J1Y83_003789"
                     /EC_number="3.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HBA7631894.1"
                     /translation="MMSAPKITFIGAGSTIFVKNILGDVFHREALKTAHIALMDIDPT
                     RLEESHIVVRKLMDSAGASGKITCHTQQKEALQDADFVVVAFQIGGYEPCTVTDFEVC
                     KRHGLEQTIADTLGPGGIMRALRTIPHLWQICEDMTEVCPDATMLNYVNPMAMNTWAM
                     YARYPHIKQVGLCHSVQGTAEELARDLNIDPATLRYRCAGINHMAFYLELERKTADGS
                     YVNLYPELLAAYDAGQAPKPNIHGNTRCQNIVRYEMFKKLGYFVTESSEHFAEYTPWF
                     IKPGREDLIERYKVPLDEYPKRCVEQLANWHKELEEYKNASRIDIKPSREYASTIMNA
                     IWTGEPSVIYGNVRNDGLIDNLPQGCCVEVACLVDANGIQPTKAGTLPSHLAALMQTN
                     INVQTLLTEAILTENRDRVYHAAMMDPHTAAVLGIDEIYALVDDLIAAHGDWLPGWLH
                     R"
     gene            5796..6704
                     /gene="melR"
                     /locus_tag="J1Y83_003790"
     CDS             5796..6704
                     /gene="melR"
                     /locus_tag="J1Y83_003790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator MelR"
                     /protein_id="HBA7631895.1"
                     /translation="MNTDTFMCSSDEKQTRSPLSLYSEYQRMEIEFRAPHIMPTSHWH
                     GQVEVNVPFDGDVEYLINNEKVNINQGHITLFWACTPHQLTDTGTCQSMAIFNLPMHL
                     FLSWPLDKDLINHVTHGMVIKSLATQQLSPFEVRRWQQELNSQNEQIRQLAIDEIGLM
                     LKRFSLSGWEPILVNKTSRTHKNSVSRHAQFYVSQMLGFIAENYDQALTINDVAEHVK
                     LNANYAMGIFQRVMQLTMKQYITAMRINHVRALLSDTDKSILDIALTAGFRSSSRFYS
                     TFGKYVGMSPQQYRKLSQQRRQTFPG"
     gene            6903..9170
                     /gene="adiA"
                     /locus_tag="J1Y83_003791"
     CDS             6903..9170
                     /gene="adiA"
                     /locus_tag="J1Y83_003791"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418541.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine decarboxylase"
                     /protein_id="HBA7631896.1"
                     /translation="MKVLIVESEFLHQDTWVGNAVERLADALSQQNVTVIKSTSFDDG
                     FAILSSNEAIDCLMFSYQMEHPDEHQNVRQLIGKLHERQQNVPVFLLGDREKALAAMD
                     RDLLELVDEFAWILEDTADFIAGRAVAAMTRYRRQLLPPLFSALMKYSDIHEYSWAAP
                     GHQGGVGFTKTPAGRFYHDYYGENLFRTDMGIERTSLGSLLDHTGAFGESERYAARVF
                     GADRSWSVVVGTSGSNRTIMQACMTDNDVVVVDRNCHKSIEQGLMLTGAKPVYMVPSR
                     NRYGIIGPIYPQEMQPETLQKKINESPLTKDKAGQKPSYCVVTNCTYDGVCYNAKEAQ
                     DLLEKTSDRLHFDEAWYGYARFNPIYADHYAMRGEPGDHNGPTVFATHSTHKLLNALS
                     QASYIHVREGRGAINFSRFNQAYMMHATTSPLYAICASNDVAVSMMDGNSGLSLTQEV
                     IDEAVDFRQAMARLYKEFTADGSWFFKPWNKEVVTDPQTGKTYHFADAPTKLLTTVQD
                     CWVMHPGESWHGFKDIPDNWSMLDPIKVSILAPGMGEDGELEETGVPAALVTAWLGRH
                     GIVPTRTTDFQIMFLFSMGVTRGKWGTLVNTLCSFKRHYDANTPLAQVMPELVEQYPD
                     TYANMGIHDLGDTMFAWLKENNPGARLNEAYSGLPVAEITPREAYNAIVDNNVELVSI
                     ENLPGRIAANSVIPYPPGIPMLLSGENFGDKNSPQVSYLRSLQSWDHHFPGFEHETEG
                     TEIIDGIYHVMCVKA"
BASE COUNT         2309 a         2492 c         2296 g         2192 t
ORIGIN      
        1 tataaaccta atgatacctg ccctgagaac aaaaaacaga ccgataaata tcaataagat
       61 aacagcaaac aaaacattaa catctgcgct gtacaaacta taaacccatc gccagagagt
      121 ctttctctct gaaaaagccg cttatcacag tgcataaatt tgccgctgct ttaatcagcc
      181 aatattcact gtgaggtatt tgctcaagcc ggtaacgacc aaacggatat ttagtcaggc
      241 tctgaaaaca gttcatacaa aacagaacgt gactgtgatc tattcagcaa aaatttaaat
      301 aggattatcg cgagggttca cacatgttat ttactatcca acttatcata atactgatat
      361 gtctgtttta tggtgccaga aaggggggta tcgcgctggg tttattaggc ggtatcggtc
      421 tggtcattct ggtcttcgtc ttccaccttc agccaggtaa accaccggtt gatgtcatgc
      481 tggttatcat tgcggtggtg gcggcatcgg cgaccttgca agcttcgggc ggtcttgatg
      541 tcatgctgca aattgccgag aagctgctgc gtcgcaaccc gaaatatgtc tcaattgtcg
      601 cgccgtttgt gacctgtacg ctgaccattc tttgcggtac gggtcatgtg gtttacacca
      661 ttctgccgat catctacgac gttgctatta agaacaacat ccgtccggaa cgtccgatgg
      721 cggcaagttc tatcggtgca cagatgggga ttatcgccag tccggtgtcg gttgcggtcg
      781 tatctttggt tgcgatgctg ggtaatgtca cctttgatgg tcgccatctt gagttcctcg
      841 acctgctggc aatcaccatt ccatcgacgt taatcggtat cctggcgatc ggtatcttca
      901 gctggttccg cggtaaagat ctggataaag acgaagagtt ccaaaaattc atctccgtac
      961 cggaaaaccg tgagtatgtt tacggtgata ccgcgacgct gctggataaa aaactgccga
     1021 aaagcaactg gctggcaatg tggatcttcc tcggggcaat cgctgtagtc gcccttcttg
     1081 gtgctgattc ggacctgcgt ccatccttcg gcggcaaacc gctgtcgatg gtactggtta
     1141 ttcagatgtt tatgctgctg accggggcgc tgattattat cctgaccaaa accaatcccg
     1201 cgtctatctc aaaaaacgaa gtcttccgtt ccggtatgat cgccatcgtg gcggtgtacg
     1261 gtatcgcatg gatggctgaa accatgttcg gtgcgcatat gtctgaaatt cagggcgtac
     1321 tgggtgaaat ggtgaaagag tatccgtggg cctatgccat tgttctgctg ctggtttcca
     1381 agtttgtaaa ctctcaggct gcggcgctgg cggcgattgt tccggtcgcg ctggcgatcg
     1441 gcgttgatcc ggcatacatc gtggcttcag caccggcttg ctacggttat tacatcctgc
     1501 cgacttatcc gagcgatctg gcagcgattc agtttgaccg ttccggcacc acccacatcg
     1561 gtcgcttcgt catcaaccac agctttattc tgccggggtt gattggtgtg agcgtatcgt
     1621 gcgtcttcgg ctggatcttc gccgcgatgt acgggttctt ataaatgcac tttgcgtgcc
     1681 gcccgtgact acgcggcacg ccattttcga ataacaaata cagagttaca ggctggaagc
     1741 tatgtcaaac aaacccttta tctaccaggc acctttcccg atggggaaag acaataccga
     1801 atactatcta ctcacttccg attacgttag cgttgccgac ttcgacggcg aaaccatcct
     1861 gaaagtggaa ccagaagccc tgaccctgct ggcgcagcaa gcctttcacg acgcttcttt
     1921 catgctccgc ccagcacacc agaaacaggt tgcggctatt cttcacgatc cagaagccag
     1981 cgaaaacgac aagtacgtgg cgctgcaatt cttaagaaac tccgaaatcg ccgccaaagg
     2041 cgtgctgccg acctgccagg ataccggcac cgcgatcatc gtcggtaaaa aaggtcagcg
     2101 cgtgtggacc ggtggcggtg atgaagaagc gctgtcgaaa ggcgtctata acacctatat
     2161 cgaagataac ctgcgctatt cacagaacgc gccgctggac atgtataaag aggtcaacac
     2221 cggcacgaac ctgcctgcgc aaatcgacct gtacgcggta gatggcgatg agtacaaatt
     2281 cctctgtgtc gctaaaggcg gcggctctgc caacaaaacg tatctctacc aggaaaccaa
     2341 agccctgctg acgcccggca aactgaaaaa cttcctcgtc gagaaaatgc gtaccctcgg
     2401 tactgcagcc tgcccgccgt accatatcgc gtttgtgatt ggcggtacgt ctgcggaaac
     2461 caacctgaaa accgtcaagt tagcaagcgc tcactattac gatgaactgc cgacggaagg
     2521 gaacgaacat ggtcaggcgt tccgcgatat ccagctggaa caggaactgc tggaagaggc
     2581 ccagaaactc ggtcttggcg cgcagtttgg cggtaaatac ttcgcgcacg acatccgagt
     2641 tatccgtctg ccacgtcacg gcgcatcctg cccggtaggc atgggagtct cctgctccgc
     2701 tgaccgtaac attaaagcga aaatcaaccg cgaaggtatc tggatcgaaa aactggaaca
     2761 caatccaggc cagtacattc cagaagaact gcgtcaggca ggtgaaggcg aagcagtgaa
     2821 agtcgacctt aaccgcccga tgaaagagat ccttgcccag ctttcgcaat acccggtatc
     2881 cacccgcttg tcgctcaccg gcaccattat cgtgggtcgc gatattgcac acgccaagct
     2941 gaaagagctg attgacgccg gtaaagaact gccgcagtac atcaaagatc acccgatcta
     3001 ctacgcgggt ccggcgaaaa cgcctgccgg ttatccatca ggttcacttg gcccaaccac
     3061 cgcaggtcgt atggactcct acgtggatct gctgcaatcc cacggcggca gcatgatcat
     3121 gctggcgaaa ggtaaccgca gtcagcaggt taccgacgcg tgccataaac acggcggctt
     3181 ctacctcggt agcatcggcg gtccggcggc ggtactggcg cagcagagca tcaagcatct
     3241 ggaatgcgtc gcttatccgg agctgggtat ggaagctatc tggaaaatcg aagtagaaga
     3301 tttcccggcg tttatcctgg tcgatgacaa aggtaacgac ttcttccagc agatcgtcaa
     3361 caaacagtgc gcgaactgca ctaagtaacc tcttcggccc agcgcctggc agcatgctgc
     3421 caggtgatcc ccctgggcca cctcttttgt gattgtaatt tcacgcttac tggtcaatag
     3481 tcagtatttt ccctgacttg cgaactcacc atgacccgca cactcaagcc gttaattctt
     3541 aacaccggcg cactggcgct aacgttaatc ctgatttata ccggcatttc ggcccatgac
     3601 aaactcacct ggctgatgga agtgacaccg gtgattattg tcgtgccgct actgcttgcc
     3661 accgctaaac gttatccgtt aacgccgctg ctctatacgc tcattttctt tcacgccatc
     3721 atcctgatgg tcggcgggca atacacctac gcgaaagtcc ccgttggttt tgaggtgcag
     3781 gaatggctgg ggttgagccg taatccgtat gacaaactgg ggcatttttt ccaggggctg
     3841 gtgcctgcac tggtggcacg agagatcctc gtgcgcggga tgtacgttcg cgggcgtaaa
     3901 atggtggcgt ttctggtctg ttgtgtcgcg ctggcgataa gcgcgatgta tgagttaatc
     3961 gagtggtggg cagcgctggc aatgggccag ggcgcagatg attttctcgg tactcaaggc
     4021 gacccgtggg atacgcaatc cgatatgttc tgcgcactac tgggcgcatt aacgacggtg
     4081 atattcctcg cccgctttca ttgccgccag ctacggcgct ttggcttaat caccagagcg
     4141 ccggaagcga ttacccctta acggtgcaac cagcctggca gccagtcgcc gtgggcggca
     4201 atcaggtcgt caacaagagc atatatttcg tcaatgccca gcacggcggc agtatgcggg
     4261 tccatcatcg cggcgtggta aacacggtcg cgattttccg taagaatagc ttctgtcagc
     4321 agcgtctgta cgttgatgtt ggtttgcatc agggcggcca gatgcgaagg tagcgtaccg
     4381 gctttggtcg gctgaatgcc attagcatca accagacagg ccacttccac gcaacatcct
     4441 tgtggcaggt tatcaatcaa gccatcgtta cggacattgc cataaatcac actcggctcg
     4501 ccagtccaga tagcgttcat gattgtgctg gcatattccc gtgacggttt aatatcaatc
     4561 cgggaggcgt ttttatactc ctccagctct ttatgccagt tcgccagctg ctcgacgcag
     4621 cgtttcgggt actcatccaa cggtacttta taacgctcaa tcaaatcctc acgacctggc
     4681 ttaataaacc acggtgtgta ctcagcaaaa tgttctgacg attctgtgac gaagtagccc
     4741 agctttttga acatttcgta acgcacaata ttctggcagc gagtattacc atgaatattt
     4801 ggcttcggtg cctgccctgc gtcataagcc gccagcaatt caggatagag attcacgtag
     4861 ctaccgtctg cggttttacg ctccagttcc aggtaaaacg ccatatggtt gatacctgcg
     4921 caacggtaac gcagcgtagc cggatcgata ttgaggtcac gcgccagctc ttccgccgtt
     4981 ccctgcaccg aatggcacag ccctacctgc ttgatatgcg gatagcgggc atacatcgcc
     5041 caggtattca tcgccattgg gttaacgtag ttgagcatgg tggcatcggg gcagacttcc
     5101 gtcatgtcct cgcaaatctg ccacaggtgc ggaatggtac gtagcgcacg cataatgccg
     5161 cccggcccca gcgtatcggc aatggtttgt tccagaccat gccgcttaca gacctcgaaa
     5221 tcagtcaccg tgcaaggttc ataaccgcca atctgaaatg ccaccaccac aaaatcagca
     5281 tcctgtaagg cttctttctg ttgggtgtgg caggtgattt tgccgctggc ccctgctgaa
     5341 tccatcagct tacgcaccac aatgtgcgac tcttccaggc gggtgggatc gatatccatc
     5401 agggcaatat gcgccgtttt cagcgcctca cgatggaaca catcaccaag aatattttta
     5461 acgaaaatcg tcgaaccagc gccgataaat gtaattttag gtgcagacat catgcagatc
     5521 tcctggcttg cttgaatgac ttcatcatgg caggcgatat cgcagaaaac ctccggtttc
     5581 ccggttgagc attcccaaaa actcagatcc taagtggcac atcaatagat ctgagtttat
     5641 gggaataaat tacctgaaaa gctgaataat cgaccccaat cttctcatcc gctctcctcg
     5701 cacctccagg gagagaaata aaaccgtgcg cctgttcgca gtcatcctcc ctcactcctg
     5761 ccataattct gatattccag gaaagagagt catccatgaa tacagatacg tttatgtgca
     5821 gcagcgacga aaagcagacc cgtagcccac tgtcgctgta ttcagaatac cagcggatgg
     5881 aaattgagtt tcgcgcaccg catatcatgc ccaccagcca ctggcatggt caggtcgaag
     5941 tgaatgtgcc tttcgatggc gatgtggaat acctgatcaa caatgaaaaa gtgaatatca
     6001 atcagggtca tatcacgctg ttctgggcct gtacaccgca ccaactaaca gataccggaa
     6061 cctgtcagag catggcgatt tttaatctgc cgatgcatct gtttctctcc tggccgctgg
     6121 ataaagacct gataaaccac gtcactcacg gcatggtgat caaatcactg gcgacacagc
     6181 aacttagccc gtttgaagtg cgccgctggc agcaggaatt aaacagtcag aacgagcaaa
     6241 ttcgccagct cgccattgat gaaattggcc tgatgctcaa gcgatttagc ctctctggct
     6301 gggaaccgat tctggtcaat aaaacctcgc gcacacacaa aaacagcgtc tcgcgccatg
     6361 cgcaatttta tgtcagccag atgctgggct ttattgccga aaactatgat caggcgctga
     6421 ccatcaacga tgtggctgag cacgtcaaac ttaacgccaa ctatgcaatg gggatattcc
     6481 agcgggtcat gcaattgacg atgaaacagt acattaccgc gatgcgcatc aaccacgttc
     6541 gcgcgttact gagcgatacc gataaaagta ttctcgatat tgccctgacg gcaggctttc
     6601 gttcaagtag tcgtttttac agcacgttcg gcaaatatgt cggcatgtca ccgcaacaat
     6661 accgcaaact tagccaacag cgccgccaga cgtttcccgg ctaaagacga tatcagtatc
     6721 agccaaaaaa atagtttagc cgtcgataat aagcagtgaa aataatttaa aataatcaca
     6781 taacatattg tttataaaaa caaatatttc aaaaatgttt gtttttcacg cgctttacag
     6841 cccgaaaagg ccggaagata cttgcccgca acgaagattc cttcataacc gggtaagcaa
     6901 tgatgaaagt attaattgtt gaaagcgagt ttctccatca agacacctgg gtcggtaacg
     6961 ccgttgagcg tctggctgat gctttaagcc agcaaaatgt taccgtgatt aaatccacct
     7021 cctttgatga tggttttgcc attctctctt caaacgaagc cattgactgc ctgatgttca
     7081 gctatcaaat ggaacatccg gacgaacatc aaaacgtcag acaattgatc ggtaagcttc
     7141 atgagcgcca acaaaacgtg ccggtcttcc tgttgggcga tcgggaaaaa gccctcgccg
     7201 caatggaccg ggatctgctg gagcttgtcg atgaattcgc ctggattctg gaagataccg
     7261 ccgactttat cgccggacgc gctgttgccg cgatgacccg ctaccgccgg cagctgttgc
     7321 cgccactgtt cagcgcgctg atgaaatata gcgacatcca tgaatattcc tgggcagcgc
     7381 caggccacca gggcggcgtt ggttttacca aaacacccgc cggacgtttc taccatgact
     7441 attatggtga aaatctgttc cgcaccgaca tgggcatcga acgcacttcc ctcggttctt
     7501 tgcttgacca tactggcgca tttggcgaaa gcgaaagata tgccgcacgc gtatttggtg
     7561 ccgatcgctc ctggtcggta gtcgtcggta cttccggctc taaccgcacc atcatgcagg
     7621 cttgcatgac cgataacgat gtagtggtcg ttgaccgtaa ctgccataaa tccatcgaac
     7681 aaggcttgat gctgacaggc gcgaaaccgg tctacatggt gccaagccgc aaccgctacg
     7741 gcattatcgg gccaatctat ccgcaggaaa tgcaacctga aaccttgcag aagaaaatca
     7801 atgaaagccc gctgactaaa gacaaagccg ggcaaaaacc gtcttactgc gtggtgacca
     7861 actgcaccta tgacggcgtg tgttataacg ctaaagaagc acaggatctg ctggaaaaaa
     7921 cctccgatcg tctgcacttt gacgaagcct ggtacggcta tgcacgtttc aacccgatct
     7981 atgccgatca ctatgccatg cgcggcgaac ctggcgatca caacggtcct accgttttcg
     8041 ccacccactc cacccacaaa ctgctgaatg cgctgtcaca ggcctcttat attcatgtac
     8101 gtgaaggtcg tggggcgatt aacttctccc gcttcaacca ggcctacatg atgcatgcca
     8161 ccacctcccc gctgtatgcc atctgcgcat ccaacgacgt ggcagtgtcg atgatggacg
     8221 gcaacagcgg cctgtcactg acccaggaag tgatcgacga agcggttgat ttccgtcagg
     8281 cgatggcgcg gctgtataaa gagttcaccg ctgacggtag ctggttcttc aaaccgtgga
     8341 acaaagaagt cgtcaccgac ccacaaaccg gcaaaaccta tcactttgct gacgcaccaa
     8401 ccaaactgct gaccaccgtt caggactgct gggtcatgca tccgggcgaa agctggcacg
     8461 gcttcaaaga tattccggat aactggagta tgctcgaccc gattaaagtc agcatccttg
     8521 ctccgggaat gggtgaagat ggtgaactgg aagaaaccgg tgttccggcg gcgctggtca
     8581 cagcctggct tggtcgccac ggcattgtgc ctacccgtac cactgacttc cagattatgt
     8641 tcctgttctc tatgggcgtg acccgtggga aatgggggac tctggttaac accctttgct
     8701 ccttcaaacg ccactatgat gccaacacac cgctggcgca ggtgatgccg gaacttgttg
     8761 aacaatatcc tgacacttac gcgaacatgg ggattcacga tctgggtgac accatgtttg
     8821 cctggctgaa agaaaacaac cctggcgcac ggttgaatga agcctattcc ggcctaccgg
     8881 tggcagaaat caccccgcgt gaagcgtaca acgcgattgt cgacaacaat gtcgaactgg
     8941 tatccattga aaatctgcca ggacgtatcg cggcaaactc agttatcccg tatccgccag
     9001 gaatcccgat gctgctgtct ggtgaaaact tcggcgataa aaacagtccg caagtaagtt
     9061 atttacgctc gctgcaatcc tgggaccatc atttccctgg atttgaacac gaaacagaag
     9121 ggacagaaat tattgacggt atttaccacg ttatgtgtgt gaaagcgtaa ccactattcc
     9181 gctgaaggcg taattgttaa ataacattac gccgcctggc ttgaggcctt ttaagtatgg
     9241 caacgttttc ataaaaattg ctgcaaacaa aaaatgtcat gctttttgc
//
LOCUS       DADSYM010000166         9249 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2415
            SAMN08039579-rid11723173.denovo.168, whole genome shotgun sequence.
ACCESSION   DADSYM010000166 DADSYM010000000
VERSION     DADSYM010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039579
            Sequence Read Archive: SRR6362533
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9249)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9249)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980162.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 03:39:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,484
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 4,347
            CDSs (with protein)               :: 4,347
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 43 of 85
            Pseudo Genes (incomplete)         :: 37 of 85
            Pseudo Genes (internal stop)      :: 19 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9249
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039579-rid11723173.denovo.168"
                     /strain="LGE2415"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8302 N 95.9628 W"
                     /collection_date="2015-08-24"
     gene            complement(163..954)
                     /gene="cpoB"
                     /locus_tag="J1Y83_003792"
     CDS             complement(163..954)
                     /gene="cpoB"
                     /locus_tag="J1Y83_003792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein CpoB"
                     /protein_id="HBA7631897.1"
                     /translation="MSSNFRHQLLSLSLLVGIAAPWAAFAQAPISSVGSGSVEDRVTQ
                     LERISNAHSQLLTQLQQQLSDNQSDIDSLRGQIQENQYQLNQVVERQKQILLQIDSLS
                     SGGAAAQSTSGDQSGVAASTTPTADAGTANAGAPVKSGDANTDYNAAIALVQDKSRQD
                     DAMVAFQNFIKNYPDSTYLPNANYWLGQLNYNKGKKDDAAYYFASVVKNYPKSPKAAD
                     AMFKVGVIMQDKGDTAKAKAVYQQVISKYPGTDGAKQAQKRLNAM"
     gene            complement(964..1485)
                     /gene="pal"
                     /locus_tag="J1Y83_003793"
     CDS             complement(964..1485)
                     /gene="pal"
                     /locus_tag="J1Y83_003793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-associated lipoprotein Pal"
                     /protein_id="HBA7631898.1"
                     /translation="MQLNKVLKGLMIALPVMAIAACSSNKNASNDGSEGMLGAGTGMD
                     ANGGNGNMSSEEQARLQMQQLQQNNIVYFDLDKYDIRSDFAQMLDAHANFLRSNPSYK
                     VTVEGHADERGTPEYNISLGERRANAVKMYLQGKGVSADQISIVSYGKEKPAVLGHDE
                     AAYAKNRRAVLVY"
     gene            complement(1520..2812)
                     /gene="tolB"
                     /locus_tag="J1Y83_003794"
     CDS             complement(1520..2812)
                     /gene="tolB"
                     /locus_tag="J1Y83_003794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001646851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system protein TolB"
                     /protein_id="HBA7631899.1"
                     /translation="MKQALRVAFGFLILWASVLHAEVRIVIDSGVDSGRPIGVVPFQW
                     AGPGAAPEDIGGIVAADLRNSGKFNPLDRARLPQQPGSAQEVQPAAWSALGIDAVVVG
                     QVTPNPDGSYNVAYQLVDTGGAPGTVLAQNSYKVNKQWLRYAGHTASDEVFEKLTGIK
                     GAFRTRIAYVVQTNGGQFPYELRVSDYDGYNQFVVHRSPQPLMSPAWSPDGSKLAYVT
                     FESGRSALVIQTLANGAVRQVASFPRHNGAPAFSPDGSKLAFALSKTGSLNLYVMDLA
                     SGQIRQVTDGRSNNTEPTWFPDSQNLAFTSDQAGRPQVYKVNINGGAPQRITWEGSQN
                     QDADVSSDGKFMVMVSSNGGQQHIAKQDLATGGVQVLSSTFLDETPSLAPNGTMVIYS
                     SSQGMGSVLNLVSTDGRFKARLPATDGQVKFPAWSPYL"
     gene            complement(2945..4210)
                     /gene="tolA"
                     /locus_tag="J1Y83_003795"
     CDS             complement(2945..4210)
                     /gene="tolA"
                     /locus_tag="J1Y83_003795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="HBA7631900.1"
                     /translation="MSKATEQNDKLKRAIIISAVLHVILFAALIWSSFDENIEASAGG
                     GGGSSIDAVMVDSGAVVEQYKRMQSQESSAKRSDEQRKMKEQQAAEELREKQAAEQER
                     LKQLEKERLAAQEQKKQAEEAAKQAELKQKQAEEAAAKAAADAKAKAEADAKAAEEAA
                     KKAAADAKKKAEAEAAKAAVEAQKKAEAAAAALKKKAEAAEAAAAEARKKAATEAAEK
                     AKAEAEKKAAAEKAAADKKAAAEKAAADKKAAEKAAAEKAAADKKAAAEKAAADKKAA
                     AAKAAAEKAAAAKAAAEADDIFGELSSGKNAPKTGGGAKGNNASPAGSGNTKNNGASG
                     ADINNYAGQIKSAIESKFYDASSYAGKTCTLRIKLAPDGMLLDIKPEGGDPALCQAAL
                     AAAKLAKIPKPPSQAVYEVFKNAPLDFKP"
     gene            complement(4275..4703)
                     /gene="tolR"
                     /locus_tag="J1Y83_003796"
     CDS             complement(4275..4703)
                     /gene="tolR"
                     /locus_tag="J1Y83_003796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015965300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin uptake protein TolR"
                     /protein_id="HBA7631901.1"
                     /translation="MARARGRGRRDLKSEINIVPLLDVLLVLLLIFMATAPIITQSVE
                     VDLPDATESQAVSSNDNPPVIVEVSGIGQYTVVVEKDRLERLPPEQVVAEVSSRFKAN
                     PKTVFLIGGAKDVPYDEIIKALNLLHSAGVKSVGLMTQPI"
     gene            complement(4707..5399)
                     /gene="tolQ"
                     /locus_tag="J1Y83_003797"
     CDS             complement(4707..5399)
                     /gene="tolQ"
                     /locus_tag="J1Y83_003797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system protein TolQ"
                     /protein_id="HBA7631902.1"
                     /translation="MTDMNILDLFLKASLLVKLIMLILIGFSIASWAIIIQRTRILNA
                     AAREAEAFEDKFWSGIELSRLYQESQGKRDNLSGSEQIFYSGFKEFVRLHRANSHAPE
                     AVVEGASRAMRISMNRELENLETHIPFLGTVGSISPYIGLFGTVWGIMHAFIALGAVK
                     QATLQMVAPGIAEALIATAIGLFAAIPAVMAYNRLNQRVNKLELNYDNFMEEFTAILH
                     RQAFTVSESNKG"
     gene            complement(5396..5800)
                     /gene="ybgC"
                     /locus_tag="J1Y83_003798"
     CDS             complement(5396..5800)
                     /gene="ybgC"
                     /locus_tag="J1Y83_003798"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system-associated acyl-CoA thioesterase"
                     /protein_id="HBA7631903.1"
                     /translation="MNTTLFRWPVRVYYEDTDAGGVVYHASYVAFYERARTEMLRHHH
                     FSQQALMAERVAFVVRKMTVEYYAPARLDDMLEIQTEITSMRGTSLVFTQRIVNAENT
                     LLNEAEVLVVCVDPLKMKPRALPKSIVAEFKQ"
     gene            complement(5950..6243)
                     /gene="ybgE"
                     /locus_tag="J1Y83_003799"
     CDS             complement(5950..6243)
                     /gene="ybgE"
                     /locus_tag="J1Y83_003799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyd operon protein YbgE"
                     /protein_id="HBA7631904.1"
                     /translation="MSKIIATLYAVMDKRPLRALSLVMALLLAGCMFWDPSRFAAKTS
                     ELEIWHGLLLMWAVCAGVIHGVGFRPQKVLWQGIFCPLLADIVLIVGLIFFFF"
     gene            complement(6243..6356)
                     /gene="cydX"
                     /locus_tag="J1Y83_003800"
     CDS             complement(6243..6356)
                     /gene="cydX"
                     /locus_tag="J1Y83_003800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-I oxidase subunit CydX"
                     /protein_id="HBA7631905.1"
                     /translation="MWYFAWILGTLLACSFGVITALALEHVESGKAGQEDI"
     gene            complement(6371..7510)
                     /gene="cydB"
                     /locus_tag="J1Y83_003801"
     CDS             complement(6371..7510)
                     /gene="cydB"
                     /locus_tag="J1Y83_003801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000568269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HBA7631906.1"
                     /translation="MIDYEVLRFIWWLLVGVLLIGFAVTDGFDMGVGMLTRFLGRNDT
                     ERRIMINSIAPHWDGNQVWLITAGGALFAAWPMVYAAAFSGFYVAMILVLASLFFRPV
                     GFDYRSKIEETRWRNMWDWGIFIGSFVPPLVIGVAFGNLLQGVPFNVDEYLRLYYTGN
                     FFQLLNPFGLLAGVVSVGMIITQGATYLQMRTVGELHLRTRATAQVAALVTLVCFALA
                     GVWVMYGIDGYVVKSTMDHYAASNPLNKEVVREAGAWLVNFNNTPILWAIPALGVVLP
                     LLTILTARMDKAAWAFVFSSLTLACIILTAGIAMFPFVMPSSTMMNASLTMWDATSSQ
                     LTLNVMTWVAVVLVPIILLYTAWCYWKMFGRITKEDIERNTHSLY"
     gene            complement(7526..9094)
                     /gene="cydA"
                     /locus_tag="J1Y83_003802"
     CDS             complement(7526..9094)
                     /gene="cydA"
                     /locus_tag="J1Y83_003802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HBA7631907.1"
                     /translation="MLDIVELSRLQFALTAMYHFLFVPLTLGMAFLLAIMETVYVLSG
                     KQIYKDMTKFWGKLFGINFALGVATGLTMEFQFGTNWSYYSHYVGDIFGAPLAIEGLM
                     AFFLESTFVGLFFFGWDRLGKVQHMCVTWLVALGSNLSALWILVANGWMQNPIASDFN
                     FETMRMEMVSFSELVLNPVAQVKFVHTVASGYVTGAMFILGISAWYMLKGRDFAFAKR
                     SFAIAASFGMAAVLSVIVLGDESGYEMGDVQKTKLAAIEAEWETQPAPAAFTLFGIPD
                     QEEETNKFAIQIPYALGIIATRSVDTPVIGLKELMVQHEERIRNGMKAYSLLEQLRSG
                     STDQAVRDQFNSMKKDLGYGLLLKRYTPNVADATEAQIQQATKDSIPRVAPLYFAFRI
                     MVACGFLLLAIIALSFWSVIRNRIGEKKWLLRAALYGIPLPWIAVEAGWFVAEYGRQP
                     WAIGEVLPTAVANSSLTAGDLIFSMVLICGLYTLFLVAELFLMFKFARLGPSSLKTGR
                     YHFEQSSTTTQPAR"
BASE COUNT         2264 a         2530 c         2211 g         2244 t
ORIGIN      
        1 tttttcgttg ctttcggttt gtttgatatc ccgtggcaac ggcggcatat attactgatt
       61 tcagacttga gcgcaacaaa aatttcgatg tagatcactc aactgcttat gattcgcacg
      121 acacgaccag aaataatgcg acttctggtc gtgtgttatg cattacatcg cgttcagacg
      181 tttttgcgcc tgtttagcgc catcagtacc agggtattta ctgataacct gctggtatac
      241 ggctttcgct tttgcggtgt cacctttgtc ctgcatgatg acgccgactt taaacatcgc
      301 atctgcagcc ttcggtgact tcggatagtt tttcactacc gaagcaaaat aatacgccgc
      361 atcatctttt ttacccttgt tgtagtttaa ctgacccagc caataattgg cgtttggcag
      421 gtaagttgaa tcagggtaat ttttgatgaa attctgaaat gccaccattg cgtcatcctg
      481 gcgggattta tcctgcacca gcgcaatagc tgcattgtaa tccgtgtttg catcaccgct
      541 tttcaccggc gcgccagcat tcgcagtacc agcatcagct gtcggcgtcg ttgatgccgc
      601 cacaccgctt tgatcgccgc tggttgattg cgccgctgca ccaccgctgc tgaggctgtc
      661 gatctgcaac aggatctgct tttgccgctc cacgacctga ttcagttgat actgattttc
      721 ctgaatctga ccacgcaggg aatcaatatc ggattgatta tcagagagtt gttgctggag
      781 ttgggttaaa agctggctgt gagcattaga aatacgctca agttgagtga cgcggtcttc
      841 gaccgagcct gagccgacac tactgattgg tgcctgagca aaagcggccc agggggccgc
      901 tataccaacc agtaacgaca gactcaatag ttgatgtctg aagttactgc tcatgcaatt
      961 ctcttagtaa accagtaccg cacgacggtt tttggcatac gccgcttcgt catgacccag
     1021 tactgcaggt ttttctttac cgtaagaaac gatggagatc tggtcagcag aaacgccttt
     1081 accctgcaga tacatcttaa cagcgttcgc acgacgttca cccagggaga tgttgtattc
     1141 cggagtacca cgttcgtccg cgtgaccttc tacggtgact ttgtaagacg ggttgctacg
     1201 caggaagttt gcgtgtgcat ccagcatttg agcgaagtca gaacggatat cgtacttgtc
     1261 cagatcgaag taaacgatgt tgttctgctg cagctgttgc atttgcagac gagcctgctc
     1321 ttcggaagac atgttgccgt tgccgccgtt cgcatccata ccagtgccgg cacccagcat
     1381 gccttcgctg ccgtcattgc tggcgttctt gttggaagaa catgccgcaa ttgccataac
     1441 aggcagagca atcatcagcc ctttcagcac tttgttcagt tgcatttcaa tgattccttt
     1501 actattcaat taattattat cacagatacg gcgaccaggc agggaatttg acctgtccat
     1561 cagttgccgg aagacgcgct ttgaaacgcc catctgtaga aaccaaattc agcacggatc
     1621 ccatcccctg agaagagctg tagattacca tagtgccgtt aggtgccaga cttggcgttt
     1681 catccaggaa cgtggacgac agaacttgta cgcctcccgt tgccagatct tgtttggcaa
     1741 tgtgctgctg cccaccgttg gagctgacca ttaccataaa tttaccgtcg ctgctgacat
     1801 ccgcatcctg gttctgcgaa ccttcccagg taatacgttg tggcgcaccg ccgttgatat
     1861 tcactttata aacctgcgga cgaccggcct ggtcagaagt gaatgccagg ttctggctgt
     1921 cagggaacca ggtcggttcg gtattgttac tgcgaccatc agtcacctgg cggatctgac
     1981 cagaagccaa atccattacg tacaggttca ggctaccggt tttcgacaag gcgaatgcca
     2041 gtttgctgcc gtctggcgag aatgcaggtg caccgttgtg acgcgggaat gaagccacct
     2101 gacgtacagc gccatttgcc agcgtctgaa taaccagcgc ggaacgaccg ctttcgaagg
     2161 tcacataagc cagtttagaa ccgtctggtg accacgccgg agacatcagc ggttgcggtg
     2221 aacggtgaac gacaaactgg ttgtaaccgt catagtcaga tacgcgcagt tcatacggga
     2281 actgaccgcc gttagtctga acaacgtagg caatacgggt acggaacgca cctttaatac
     2341 cggtcagttt ttcaaatact tcgtcactgg cggtatgacc agcataacgc agccactgct
     2401 tgttcacttt gtacgagttc tgagcaagta cagtacccgg tgcgccgcca gtgtcaacaa
     2461 gttgataagc aacattgtaa gaaccatccg gattcggagt gacctgaccg acaaccacag
     2521 cgtcaatgcc cagcgcggac catgcagctg gttgtacttc ctgagcacta cccggctgct
     2581 gtggcagacg agcacgatct aacggattaa atttaccgct gttacgcaag tcagcagcaa
     2641 cgatgccgcc aatatcttca ggtgccgcac caggccccgc ccactggaaa ggaacaacac
     2701 caataggacg accggaatct acaccgctgt cgatcacaat gcggacttca gcatgcagaa
     2761 ctgatgccca cagtatgaga aaaccaaatg ctactcgtaa tgcctgcttc atcatatctc
     2821 ccttatctgg accgatggcc cacgataatt tagcagaatg ttaacaaact aaaatacaca
     2881 aaactaccag aaccccgtgg caaccggtgc ctgatgttga ccgtcggaac agtcaacatc
     2941 gcgattacgg tttgaagtcc aatggcgcgt ttttgaacac ttcatatact gcctggcttg
     3001 gtggtttcgg gatcttcgca agtttagctg ctgccaacgc agcttgacaa agtgcgggat
     3061 cgccaccttc aggtttgata tccagtaaca taccatcggg tgccagtttt atgcgcagcg
     3121 tacaggtttt gcctgcatag gacgatgcgt catagaactt actttcgata gcagatttaa
     3181 tctgcccggc atagttattg atatcggccc ctgatgcgcc attgttttta gtattaccac
     3241 tcccggcagg cgaagcattg ttccctttcg cccctccccc cgttttcggt gcattcttac
     3301 cagagcttag ctcaccgaaa atatcatctg cctctgcggc agcttttgct gcagcggctt
     3361 tttcagctgc tgcttttgcc gctgccgctt ttttgtctgc ggcggctttt tctgccgctg
     3421 ctttcttatc agctgctgcc ttttcagcag ccgctttttc tgctgctttt ttgtcggctg
     3481 ccgctttctc tgctgccgct ttcttatcag ctgcagcctt ttcagcagcc gctttcttct
     3541 cagcttctgc cttggctttt tcagcagctt cggttgccgc tttctttctt gcttcagctg
     3601 cagctgcttc tgccgcttca gctttcttct tcagcgccgc agcagcagcc tcggcttttt
     3661 tctgcgcttc gactgcggct ttggcggctt ctgcttctgc ttttttcttc gcgtctgccg
     3721 ccgctttctt cgctgcttct tccgcagctt tagcatccgc ttcggccttc gctttagcat
     3781 ctgccgccgc tttcgctgcc gcctcttccg cttgcttctg ctttaactcg gcctgttttg
     3841 cggcttcttc agcctgcttt ttctgctcct gagccgctaa ccgctctttc tcaagttgct
     3901 tcaggcgttc ctgttcagcc gcttgtttct cacgcagttc ttcagcagcc tgctgctcct
     3961 tcatcttgcg ctgctcatca gaacgcttcg cgcttgattc ctggctttgc atacgtttgt
     4021 actgctcaac taccgcacct gaatcaacca tgacagcgtc gatggacgaa ccaccgccgc
     4081 ctccggctga agcttctata ttctcatcga acgaactcca gatcagcgcc gcaaataaga
     4141 tgacatgcag cactgctgaa ataattatcg cccgcttgag cttgtcgttt tgttcggttg
     4201 cctttgacac tctcggtttc caaaaactgt tcgcctgtta cccgctctct ttcaagcaag
     4261 ggaaacgcag atgtttagat aggctgcgtc attaaaccaa ccgatttcac acccgcacta
     4321 tgtaacaagt tcagtgcttt aattatttca tcgtaaggca catcttttgc gccaccgatc
     4381 agaaagaccg ttttcgggtt ggccttgaaa cggctggaca cttccgccac cacctgctct
     4441 ggtggtaaac gctccagacg atctttctca accaccacgg tgtactgacc aataccagac
     4501 acttcaacaa tcactggcgg attatcgtta ctgctcaccg cctgtgattc agtagcgtct
     4561 ggcagatcga cctccacgct ctgggtgatg atgggcgctg tcgccataaa gatcagcaac
     4621 agcaccagca gtacgtccag caacggtaca atgttgattt cggacttgag atcgcgacga
     4681 cctcgtccac gcgctctggc catggcttac cccttgttgc tctcgctaac ggtaaacgcc
     4741 tggcggtgca gaatcgcggt aaactcttcc ataaagttgt cgtaattcag ttccagtttg
     4801 tttacgcgct ggtttaggcg gttataagcc attaccgccg gaattgcggc gaacaaacca
     4861 attgccgtcg caatcaacgc ttctgcgata ccgggcgcaa ccatttgcag tgttgcttgt
     4921 tttaccgccc cgagggcgat aaaggcgtgc atgatccccc agaccgtacc aaacagacca
     4981 atatacgggc tgatggagcc aaccgtaccg aggaacggaa tgtgcgtttc cagattttca
     5041 agttcacgat tcatggaaat acgcatcgca cgcgacgccc cttccactac ggcttccggc
     5101 gcatggctat tggcacgatg caggcgcaca aactctttga agccgctgta aaagatttgt
     5161 tccgaaccgc tcagattatc ccgtttcccc tggctctctt gatagaggcg agagagttcg
     5221 attccagacc agaatttatc ttcaaacgct tcggcttcgc gcgccgctgc gttaagaata
     5281 cgggtccgct ggataataat ggcccaggat gcgattgaaa aaccaatcaa aatcaacatg
     5341 ataagtttaa ccagaaggct agccttcagg aacaaatcaa ggatattcat gtcagtcact
     5401 gcttaaactc cgcgacaata gacttgggaa gcgcacgagg cttcattttg agtgggtcaa
     5461 cgcaaacaac cagaacctct gcttcattca acaaagtatt ctcggcgttg acaatacgtt
     5521 gcgtgaaaac caaagaggtg ccacgcattg atgttatttc agtctgtatt tcgagcatat
     5581 cgtcgagccg cgcaggtgcg taatattcca ccgtcatttt acgtaccaca aaggcaacgc
     5641 gttcagccat cagcgcctgt tgactgaaat gatgatgacg cagcatctct gtgcgtgctc
     5701 tttcataaaa agcgacgtaa ctggcgtggt acaccacacc accggcatcg gtatcttcat
     5761 agtagacgcg aaccggccat cgaaacagcg ttgtattcac tttacatccc ggtaatgcaa
     5821 caaaagttag agcttttaaa cgctgctact atacgcgcgc gattgaggtt tgggaatgag
     5881 ggcaagttaa gggagcgtaa aaatttatgg gcctgactcg gcccataaat ttttaaagag
     5941 aattctgatt taaaagaaga agaaaatcag cccgacaatg agaacaatat cggcaagcaa
     6001 tgggcaaaaa atcccttgcc agagaacctt ctgcggacga aagcccacgc cgtgaatcac
     6061 accagcacag acggcccaca tcagcagtaa accatgccag atttccagtt cactggtctt
     6121 cgcggcgaaa cgtgatgggt cccaaaacat acatcctgct aacagaagtg ccatcaccaa
     6181 ggaaagcgcc cgcagggggc gcttgtccat taccgcatac aaggttgcga taatcttact
     6241 catcagatgt cttcttgacc ggctttgcct gattcgacgt gctcaagcgc cagcgcggtg
     6301 attaccccaa acgaacaggc aagaagcgtt cccagaatcc atgcgaaata ccacattttt
     6361 agctccttac ttagtacaga gagtgggtgt tacgttcaat atcttctttg gtgatacgac
     6421 cgaacatttt ccagtaacac caggcggtgt agagcagaat gatcggtacc agaaccaccg
     6481 caacccaggt catgacgtta agcgtcagct ggctggaagt tgcatcccac attgtcagac
     6541 ttgcgttcat catggtgctg gacggcatca caaacgggaa cattgcgata ccggcagtca
     6601 ggatgatgca ggccagcgtc agggaggaga acacaaacgc ccacgcggct ttatccatac
     6661 gtgcagtcag gatggtcagc agcggcagaa ccacacccag tgccggaata gcccacagaa
     6721 ttggcgtgtt gttgaagtta accagccatg cgccagcttc acgaaccact tctttattca
     6781 gcgggttaga ggctgcgtaa tggtccattg tcgatttcac gacataacca tcgataccgt
     6841 acatcaccca tacgccagcc agtgcgaagc agaccagcgt caccagtgcc gccacctgag
     6901 ccgttgcacg ggtacgcagg tgcaattcgc ccacggtacg catttgcaga taggttgcgc
     6961 cctgagtaat gatcatccct acgctcacca cacctgccag caggccgaac gggttaagca
     7021 gctggaagaa gttaccggtg tagtacagac gcagatattc atcaacgttg aatggtacgc
     7081 cctgcaacag gttaccgaac gctacaccaa tcaccagcgg cggaacgaag ctaccaatga
     7141 agatgcccca gtcccacatg ttacgccagc gggtttcttc aatcttggag cggtagtcaa
     7201 aaccgaccgg acggaagaac aaagacgcca gcacgaggat catcgccaca tagaagccgg
     7261 agaacgcagc ggcatagacc atcggccagg cagcaaacag tgcgccgcct gcggtgatca
     7321 gccaaacctg gttaccgtcc cagtgtggtg cgatggagtt aatcataatt cgacgctcgg
     7381 tgtcgttacg accgaggaaa cgggtgagca tgcccacccc catgtcgaaa ccgtcagtga
     7441 ctgcaaaacc aattagcaga acgccaacca gcagccacca gataaaacgc aatacttcat
     7501 aatcgatcat ttgacgactc ctgtcttagc gtgccggctg agtagtcgtg gaagactgct
     7561 caaagtgata gcgaccggtt ttcaggctgc ttgggccgag gcgtgcaaac ttgaacatta
     7621 agaacaattc tgccaccagg aacagggtat acaggccgca aatcagcacc atcgagaaga
     7681 tgagatcgcc tgcggtcagt gacgagttcg ccacagctgt cggcagcact tcaccgatag
     7741 cccacggttg gcggccatat tcagccacga accagcccgc ttctacagca atccacggca
     7801 gcggaatacc gtacagcgcg gcgcgcagca gccatttttt ctcgccaatg cggtttcgga
     7861 tgacactcca gaaagagagc gcgatgattg ccagcagcag gaagccacac gccaccatga
     7921 tacggaacgc aaagtacagc ggcgctacac gcggaatgga gtctttggtt gcctgttgaa
     7981 tctgagcttc agtcgcatca gccacgtttg gcgtatagcg tttcagcagc agaccgtaac
     8041 cgagatcttt cttcatgcta ttgaactggt cacgaacggc ctggtcagta gaaccagaac
     8101 gcagttgttc gagcagagag tacgccttca tcccgttacg aatgcgttct tcatgctgca
     8161 ccatcagttc tttcagaccg ataactggag tatccacgga acgcgttgca atgatgccca
     8221 gcgcgtaagg gatctggatc gcaaatttgt tcgtttcctc ttcctgatca ggaatgccga
     8281 acagagtaaa ggcagcaggt gcaggttgcg tttcccactc ggcttcgata gcagccagtt
     8341 tggttttctg cacgtcgccc atttcgtagc cggattcatc acccagaaca ataacagaca
     8401 gaacggcagc cataccgaag ctggcagcga tagcaaagga gcgtttagcg aaggcgaagt
     8461 cacgaccttt cagcatatac catgcgctga taccgaggat gaacattgcg ccagtcacat
     8521 aaccagaagc tacggtgtga acgaatttca cctgagcaac cgggttaagc accagctcgg
     8581 agaagctcac catctccata cgcatagttt caaagttgaa atcggacgcg attgggtttt
     8641 gcatccagcc gtttgcaacc agaatccaca gtgcggacag gtttgaaccg agcgccacca
     8701 gccaggtgac acacatatgc tgaactttac ccagacgatc ccaaccgaag aagaacagac
     8761 ctacaaaggt ggattcgagg aagaaggcca tcagaccttc gattgccagc ggcgcaccga
     8821 agatatcccc tacatagtgg gaatagtaag accagttagt cccgaactgg aactccatgg
     8881 tcagaccggt agccacaccc agagcaaagt tgataccaaa caacttgccc cagaacttgg
     8941 tcatatcttt ataaatctgt ttgccggaaa ggacgtagac cgtttccata atggccagca
     9001 ggaacgccat accgagcgtc agtggcacaa aaaggaagtg gtacatcgcg gtcaaggcaa
     9061 actgtaagcg cgacagttcg actatatcta acatcatgac tccttgctca tcgcatgaag
     9121 actccgagag tgaaccccgt tagaaagggt cacacgcatg ccccaataca aaattatttg
     9181 ctcgcctcta tcgttgccat ttcatcaaaa gaggaaaaga caatgataaa aaagttacaa
     9241 atgtgttaa
//
LOCUS       DADSYM010000167         9053 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2415
            SAMN08039579-rid11723173.denovo.169, whole genome shotgun sequence.
ACCESSION   DADSYM010000167 DADSYM010000000
VERSION     DADSYM010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039579
            Sequence Read Archive: SRR6362533
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9053)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9053)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980162.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 03:39:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,484
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 4,347
            CDSs (with protein)               :: 4,347
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 43 of 85
            Pseudo Genes (incomplete)         :: 37 of 85
            Pseudo Genes (internal stop)      :: 19 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9053
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039579-rid11723173.denovo.169"
                     /strain="LGE2415"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8302 N 95.9628 W"
                     /collection_date="2015-08-24"
     gene            147..4397
                     /gene="fdeC"
                     /locus_tag="J1Y83_003803"
     CDS             147..4397
                     /gene="fdeC"
                     /locus_tag="J1Y83_003803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000092542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intimin-like adhesin FdeC"
                     /protein_id="HBA7631908.1"
                     /translation="MSRYKTDNKQPRFRYSVLARCVAWANISVQVLFPLAVTFTPVMA
                     ARAQHAVQPRLSMENTTVTADNNVEKNVASLAANAGTFLSSQPDSDATRNFITGMATA
                     KANQEIQEWLGKYGTARVKLNVDKNFSLKDSSLEMLYPIYDTPTNMLFTQGAIHRTDD
                     RTQSNIGFGWRHFSENDWMAGVNTFIDHDLSRSHTRIGVGAEYWRDYLKLSANGYIRA
                     SGWKKSPDVEDYQERPANGWDIRAEGYLPAWPQLGASLMYEQYYGDEVGLFGKDKRQK
                     DPHAITAEVNYTPVPLLTLSAGHKQGKSGENDTRFGLEVNYRIGEPLEKQLDTDSIRE
                     RRMLAGSRYDLVERNNNIVLEYRKSEVIRIALPERIEGKGGQTVSLGLVVSKATHGLK
                     NVQWEAPSLLAAGGKITGQGNQWQVTLPAYQAGKDNYYAISAIAYDNKGNASKRVQTE
                     VVISGAGMSADRTALTLDGQSRIQMLANGNEQKPLVLSLRDAEGQPVTGMKDQIKTEL
                     TFKPAGNIVTRTLKATKSQAKPTLGEFTETEAGVYQSVFTTGTQSGEATITVSVDDMS
                     KTVTAELRATMMDVANSTLSANEPSGDVVADGQQAYTLTLTAVDSEGNPVTGEASRLR
                     LVPQDTNGVTVGAISEIKPGVYSATVSSTRAGNVVVRVFSEQYQLGTLQQTLKFVAGP
                     LDAAHSSITLNPDKPVVGGTVTAIWTAKDANDNPVTGLNPDAPSLSGAAAAGSTASGW
                     TDNGDGTWTAQISLGTTAGELDVMPKLNGQDAAANAAKVTVVADALSSNQSKVSVAED
                     HVKAGESTTVTLVAKDAHGNAISGLSLSASLTGTASEGATVSSWTEKGDGSYVATLTT
                     GGKTGELRVMPLFNGQPAATEAAQLTVIAGEMSSANSTLVADNKTPTVKTTTELTFTV
                     KDAYGNPVTGLKPDAPVFSGAASTGSERPSAGNWTEKGNGVYVSTLTLGSAAGQLSVM
                     PRVNGQNAVAQPLVLNVAGDASKAEIRDMTVKVNNQLANGQSANQITLTVVDSYGNPL
                     QGQDVTLTLPQGVTSKTGNTVTTNAAGKVDIELMSTVAGEHNISASVNGAQKTVTVKF
                     NADASTGQANLQVDTAVQKVANGKDAFTLTANVEDKNGNPVPGSLVTFNLPRGVKPLT
                     GDNVWVKANDEGKAELQVVSVTAGTYEITASAGNDQPSNAQSVTFVADKTTATISSIE
                     VIGNRAVADGKTKQTYKVTVTDANNNLLKDSEVTLTASPENLVLTPNGTATTNEQGQA
                     IFTATTTVAATYTLTAQVSQNNGQVSTKTAESKFVADDKNAVLAASPERVDSLVADGK
                     TTATLTVTLMSGVNPVGGTMWVDIEAPEGVTEADYQFLPSKNDHFASGKITRTFSTNK
                     PGTYTFTFNSLTYGGYEMKPVTVTINAVPADTEGAEEK"
     gene            complement(4522..5379)
                     /locus_tag="J1Y83_003804"
     CDS             complement(4522..5379)
                     /locus_tag="J1Y83_003804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308364.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBA7631909.1"
                     /translation="MIRQKILQQLLEWIECNLEHPISIEDIAQKSGYSRRNIQLLFRN
                     FMHVPLGEYIRKRRLCRAAILVRLSAKSMLDIALSLHFDSQQSFSREFKKLFGCSPRE
                     YRHRDYWDLANIFPSFLIRQQQKTECRLVNFPETPIFGNSFKYDIEVSNKLPDEEVKL
                     RRHHLVRCMKNFKTDIYFVSTFEPSTKSVDLLTVETFAGTVCKQTDSEMPKEWTINRG
                     LYASFRYEGEWEHYPEWARNLYLMELPARGLARVNGSDIERFYYNENFVEIDSNNIVC
                     EIFIPVRPV"
     gene            5627..6496
                     /locus_tag="J1Y83_003805"
     CDS             5627..6496
                     /locus_tag="J1Y83_003805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308365.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HBA7631910.1"
                     /translation="MEFSVLSNNLKMPMMGFGVFQVTDKDVCKQSVLNAIRTGYRLID
                     TAAVYGNEDAVGEAVREAISEGLCTREELFITSKLWVQDMLNQDIAAAGIEASLKKSG
                     LEYFDLYLLHQAMRDYFSAWRALEDAYEEGKLKAIGVSNFYPHVLANFCETVRVKPMV
                     NQVELHPYFAQPEALATMKYYNVQPEAWAPLGGGRHKPFENNLLQSIADAHQKSISQV
                     ILRWNIQRGVVVIPKSTHQQRIEENFAIWDFSLTEKEMAQISSLDLGYVRESVKHFNP
                     EFVRGCLAVKIHD"
     gene            complement(6656..7249)
                     /gene="rclC"
                     /locus_tag="J1Y83_003806"
     CDS             complement(6656..7249)
                     /gene="rclC"
                     /locus_tag="J1Y83_003806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414835.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="reactive chlorine species resistance protein
                     RclC"
                     /protein_id="HBA7631911.1"
                     /translation="MEKYLHLLSRGDKIGLTLIRLSIAIVFMWIGLLKFVPYEADSIT
                     PFVANSPVMSFFYEHPEDYKQYLTHEGEYKPEARAWQTANNTYGFSNGLGVVEVIIAL
                     LVLANPVNRWLGLLGGLMAFTTPLVTLSFLITTPEAWVPALGDAHHGFPYLSGAGRLV
                     LKDTLMLAGAVMIMADSARDILKQRSNESSSTLKTEY"
     gene            complement(7261..7497)
                     /locus_tag="J1Y83_003807"
     CDS             complement(7261..7497)
                     /locus_tag="J1Y83_003807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414837.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HBA7631912.1"
                     /translation="MFKKSVLFATLLSGVMAFSTNADDKIILKHISVSSVSASPTVLE
                     NAIADMARKYNASSWKVTSMRIDNNSTATAVLYK"
     gene            complement(7606..8931)
                     /locus_tag="J1Y83_003808"
     CDS             complement(7606..8931)
                     /locus_tag="J1Y83_003808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridine nucleotide-disulfide oxidoreductase"
                     /protein_id="HBA7631913.1"
                     /translation="MNKYQAVIIGFGKAGKTLAVTLAKAGWRVALIEQSNAMYGGTCI
                     NIGCIPTKTLVHDAQQHTDFVRAIQRKNEVVNFLRNKNFHNLADMPNIDVIDGQAEFI
                     NNHSLRVHRPGGNLEIHGEKIFINTGAQAVVPPIPGITTTPGVYDSTGLLNLKELPGH
                     LGILGGGYIGVEFASMFANFGSKVTILEAASLFLPREDRDIADNIATILRDQGVDIIL
                     NAHVERISHHENQVQVHSEHAQLAVDALLIASGRQPATASLHPENAGIAVNERGAIVV
                     DKQLHTTANNIWAMGDVTGGLQFTYISLDDYRIVRDELLGEGRRSTDDRKNVPYSVFM
                     TPPLSRVGMTEERARESGADIQVVTLPVAAIPRARVMNDTRGVLKAIVDNKTQRILGA
                     SLLCVDSHEMINIVKMVMDAGLPYSILRDQIFTHPSMSESLNDLFSLVK"
BASE COUNT         2487 a         2032 c         2229 g         2305 t
ORIGIN      
        1 attaggtggc ggtgtacttt cttacataaa ggtatttcct tttctgtgga aaaggaaacc
       61 gggaaatccc cggtttttct gacaagcaga cgccattatt tgtgtctgcc tatgttcgtt
      121 aattcgttca tcaggaaatt atctcaatgt cacgttataa aacagacaat aaacagccac
      181 gatttcgtta ttcagttctg gcccgctgcg tggcgtgggc aaatatctct gttcaggttc
      241 tttttccact cgcagtcacc tttacgccag taatggcagc acgtgcgcag catgcggttc
      301 agccacggtt gagcatggaa aatactacgg taactgctga taataacgtg gagaaaaatg
      361 tcgcgtcgct tgccgctaat gccgggacat ttttaagcag tcagccagat agcgatgcga
      421 cacgtaactt tattaccgga atggccaccg ctaaagctaa ccaggaaatt caggagtggc
      481 tcgggaaata cggtactgcg cgcgtcaaac tgaatgtcga taaaaatttc tcgctgaagg
      541 actcttcgct ggaaatgctt tatccgattt atgatacacc gacaaatatg ttgttcactc
      601 agggggcaat acatcgtacc gacgatcgta ctcagtcaaa tattggtttt ggctggcgtc
      661 atttttcaga aaatgactgg atggcggggg tgaatacttt tatcgatcat gatttatccc
      721 gtagtcatac ccgcattggt gttggtgcgg aatactggcg tgattatttg aaactgagcg
      781 ccaatggtta tatccgggct tctggctgga aaaaatcgcc ggatgttgag gattatcagg
      841 aacgcccggc gaatggctgg gatattcgtg ctgagggcta tttacctgcc tggccgcagc
      901 ttggcgcaag cctgatgtat gaacagtatt atggcgatga agtcgggctg tttggtaaag
      961 ataaacgcca gaaagatcca catgcgatta ccgctgaagt gaattacacg ccagtgcctc
     1021 ttctgaccct gagtgccggg cataagcagg gcaagagtgg tgagaatgac actcgctttg
     1081 gcctggaagt taattatcgg attggcgaac ctctggaaaa acaactcgat acagacagca
     1141 ttcgcgagcg tcgaatgctg gcaggcagcc gctatgacct ggttgagcgt aataacaata
     1201 tcgttcttga gtatcgcaaa tctgaagtga tccgtattgc tctgcctgag cgtattgaag
     1261 gcaagggcgg ccagacggtt tccctggggc ttgtggtcag caaagcaact cacggtctga
     1321 aaaatgtgca atgggaagcg ccgtctttgc tggccgcagg cggaaaaatt acggggcagg
     1381 gcaatcagtg gcaagtgacg ctcccggctt atcaggcagg caaagacaat tattatgcga
     1441 tttcagcgat tgcctacgat aacaaaggca atgcctcgaa acgtgtgcag acagaagtgg
     1501 ttattagcgg agctggtatg agcgccgatc gtacggcgtt aacgcttgac ggtcagagcc
     1561 gtattcaaat gcttgctaac ggtaatgagc aaaagccgtt ggtgctgtct ctgcgcgacg
     1621 ccgaggggca gccagtcacg ggcatgaaag atcagatcaa gactgaacta accttcaaac
     1681 cggctggaaa tattgtgact cgtaccctga aggccactaa atcacaggca aagccaacac
     1741 tgggtgagtt caccgaaact gaagcagggg tgtatcagtc tgtctttact accggaacgc
     1801 agtcaggtga ggcaacgatt actgttagcg ttgatgacat gagcaaaact gtcactgcag
     1861 aactgcgggc cacgatgatg gatgtggcaa actccaccct gagcgctaac gagccgtcag
     1921 gtgatgtggt tgctgatggt cagcaagcct acacgctgac actgacagcg gtggactccg
     1981 agggtaatcc ggtgacggga gaagccagcc gcctgcgact tgttccgcaa gacactaatg
     2041 gtgtaaccgt tggtgccatt tcggaaataa aaccaggggt ttacagcgcc acggtttctt
     2101 cgacccgtgc cggaaacgtt gttgtgcgtg tcttcagcga gcagtatcag ctgggcacat
     2161 tacaacaaac gctgaagttt gttgccgggc cgcttgatgc agcacattcg tccatcaccc
     2221 tgaatcctga taaaccggtg gttggcggta cagttacggc aatctggacg gcaaaagatg
     2281 ctaatgacaa ccctgtaact ggcctcaatc cggatgcacc gtcattatcg ggcgcagctg
     2341 ctgctggttc tacggcatca ggctggacgg ataatggcga cgggacctgg actgcgcaga
     2401 tttctctcgg cactacggcg ggtgaattag acgttatgcc gaagctcaat gggcaggacg
     2461 cggcagcaaa tgcggcaaaa gtaaccgtgg tggctgatgc attatcttca aaccagtcga
     2521 aagtctctgt cgcagaagat cacgtaaaag ccggtgaaag cacaaccgta acgctggtgg
     2581 cgaaagatgc gcatggcaac gctatcagtg gtctttcgtt gtcggcaagt ttgacgggga
     2641 ccgcctctga aggggcgacc gtttccagtt ggaccgaaaa aggtgacggt tcctatgttg
     2701 ctacgttaac tacaggcgga aagacgggcg agcttcgtgt catgccgctc ttcaacggcc
     2761 agcctgcagc caccgaagcc gcgcagctga ctgttattgc cggagagatg tcatcagcga
     2821 actctacgct tgttgcggac aataaaactc caacggttaa aacgacgacg gaactcacct
     2881 tcaccgtgaa ggatgcgtac gggaatccgg tcaccgggct gaagccagat gcaccagtgt
     2941 ttagtggtgc cgccagcacg gggagtgagc gtccttcagc aggaaactgg acagagaaag
     3001 gtaatggggt ctacgtgtcg accttaacgc tgggatctgc cgcgggtcag ttgtctgtga
     3061 tgccgcgagt gaacggccaa aatgccgttg ctcagccact ggtgctgaat gttgcaggtg
     3121 acgcatctaa ggctgagatt cgtgatatga cagtgaaggt taataaccaa ctggctaatg
     3181 gacagtctgc taaccagata accctgaccg tcgtggacag ctatggtaac ccgttgcagg
     3241 ggcaagacgt tacgctgact ttaccgcagg gtgtgaccag caagacgggg aatacagtaa
     3301 caaccaatgc ggcagggaaa gtggacattg agcttatgtc aacggttgcg ggagaacaca
     3361 atatttccgc ttcggtgaat ggtgctcaga agacggtcac ggtgaaattc aacgcggatg
     3421 ccagcaccgg tcaggcaaac ctgcaggtag acaccgccgt tcaaaaagtg gcaaacggca
     3481 aagatgcctt tacgctgacg gcgaacgttg aggataaaaa tggtaaccct gttccaggga
     3541 gcctggtgac ctttaatctg ccccggggtg tcaagccgct tacaggcgat aatgtgtggg
     3601 tgaaagccaa cgatgagggg aaagcggagt tgcaggtggt ttccgtgact gccggaacct
     3661 atgagatcac ggcgtcagca ggaaatgacc agccttcgaa tgcgcagtct gtaacgtttg
     3721 tggccgataa gactacggcg accatctcca gtattgaggt gattggcaac cgtgcagtgg
     3781 cggacggcaa aaccaaacag acgtataaag ttacggtgac tgatgccaat aacaacctgc
     3841 tgaaagatag cgaagtgacg ctgactgcca gcccggaaaa tttagttctg actcccaatg
     3901 ggacggcgac aacgaatgag caagggcagg ctattttcac cgccaccaca actgttgcgg
     3961 cgacatatac actcacggcg caagtgagtc agaacaacgg tcaggtatca acgaaaaccg
     4021 ccgaatctaa attcgtcgcg gatgataaaa acgcggtgct cgctgcatct ccagagcgtg
     4081 tagattctct ggtggcggac gggaagacta ctgcaacact gacggttact ctgatgtcgg
     4141 gtgtcaaccc cgtaggagga accatgtggg tcgacattga ggctccggaa ggggtgacag
     4201 aggcggatta tcagttcctg ccgtcgaaaa atgaccattt cgcgagcggg aaaatcacgc
     4261 gtacatttag taccaacaag ccaggtacat acacattcac attcaactct ttgacatatg
     4321 gagggtatga aatgaaacca gtgactgtga caattaacgc cgttcctgca gatactgaag
     4381 gcgctgagga gaaataaaaa cgacgatgta ataaataata atggcgttga catgcgcagc
     4441 gccattattt actgaatacc taccctggta ccgattagac ggtggcaggg tagggtaaat
     4501 cttatgtgat ggagttacag gctaaaccgg gcgaacgggg ataaaaattt cgcaaacaat
     4561 gttattgcta tcaatttcga cgaagttttc attgtaataa aatcgctcaa tatcactgcc
     4621 gttcactctg gctaaccccc tggcaggtaa ttccatcaga tagaggtttc gcgcccattc
     4681 agggtagtgc tcccactcac cttcatagcg gaaagaggca tacagtcctc ggttaattgt
     4741 ccactctttc ggcatctctg aatcggtttg tttgcatacc gtaccggcaa aagtttcaac
     4801 tgtgagcaaa tcgaccgatt ttgttgatgg ttcgaacgtg gagacgaaat agatatccgt
     4861 cttaaaattc ttcatacatc tgactaaatg atgacgtcgc agtttgactt cttcatccgg
     4921 taatttattc gacacttcaa tatcatattt aaatgaattg ccaaaaatag gtgtctcagg
     4981 aaagttgact aatctacact ccgtcttttg ctgttgacgt attaaaaaag aagggaagat
     5041 atttgcgaga tcccaataat cacggtggcg gtattcacgg ggagagcagc cgaataactt
     5101 tttaaattcg cggctgaatg actgctgtga atcaaaatgc aaagagagtg caatatcaag
     5161 catagatttc gcggagagcc ggacaagaat ggcggcacga caaagcctgc gtttgcgaat
     5221 gtattctccc aaaggcacat gcatgaaatt tcggaacaga agctggatgt tgcgtctgct
     5281 gtagccagat ttctgtgcga tatcttcgat tgaaataggg tgctcaagat tgcactcaat
     5341 ccactccagg agctgctgta gaatcttctg cctgatcatc acatactccg ttaattcttt
     5401 gtcatatcgc acattttcgt tatgtcctgg gccctaagtg tatcaaaaac agcaagtccc
     5461 aaggcaaaag cactgatagc gtctgcttca ttgccccaaa aatagagagg tttcagtcct
     5521 acattattaa tgaatttttt gcataagtga tatcaaaatc cacatactaa ttcgaggtta
     5581 cgttttaacg tagactcatt gctcatacct aatggaggga ctgacagtgg aattttccgt
     5641 attaagtaac aatctgaaaa tgccgatgat gggatttggt gtttttcagg ttaccgataa
     5701 agacgtgtgc aaacagtcag tgttgaatgc tatccgcacg ggttatcgac tcatcgacac
     5761 tgcagcggta tacggtaatg aagatgccgt cggtgaagcc gttcgtgaag ccatatctga
     5821 aggtttatgt acccgagaag agttatttat aacgtctaaa ttgtgggtgc aggatatgtt
     5881 gaatcaggat attgcagcag caggtattga agcatcatta aaaaaatcgg gactagaata
     5941 cttcgatctc tatttattgc accaggccat gcgcgattat tttagcgcgt ggcgtgcact
     6001 cgaagatgcc tatgaagagg gcaaattaaa agcgattggg gtttccaatt tctaccctca
     6061 tgttctggcg aacttttgtg aaacggtaag agttaaaccg atggtcaacc aggtcgagtt
     6121 gcatccctat tttgcccaac cagaggcgct ggcaaccatg aagtattata acgtgcagcc
     6181 tgaagcatgg gctccgttag gtggtggacg acataaaccc tttgaaaata atctgcttca
     6241 gagtattgca gatgcccatc aaaaatcgat ttctcaagtc attctgcgtt ggaatattca
     6301 acggggagtg gtcgttattc cgaaatcgac acatcaacag cgtatcgaag aaaattttgc
     6361 tatctgggat ttctcactga cagagaaaga aatggcacaa attagttcgc ttgatttggg
     6421 ttatgttagg gaatcggtaa aacattttaa tcctgagttt gttcgtggtt gtcttgctgt
     6481 aaaaatacat gattgatatt aatcacttat tttacttgag acgacaagaa tcttttaaca
     6541 ggggagtgat attgatcttc actctgtcat atctccggta atatggcgtc aggcttcatc
     6601 atcagaaagg gagcctgaca cctgaaaaag tgaacaacag acagtgttcg gattatcaat
     6661 attcagtttt taacgttgaa ctggattcat tactgcgttg tttaagaata tcccgcgccg
     6721 aatctgccat tatcatgaca gcacctgcca gcatcagagt atctttcaat accagacgac
     6781 cagcaccgga taaataaggg aaaccatgat gagcgtcacc caatgcgggt acccatgcct
     6841 ccggagtggt gattaaaaat gagagtgtta ccaacggtgt ggtaaatgcc atcagccctc
     6901 ccagtaaacc taaccagcga ttgacagggt tagccaaaac cagcagagca ataatcacct
     6961 ccacgacgcc aagaccgttg gaaaaaccat aggtattatt ggccgtttgc catgcccttg
     7021 cttctggttt gtattcgcct tcgtgagtca gatactgttt gtagtcttcc gggtgttcat
     7081 aaaagaacga cattactgga ctgtttgcga cgaatggtgt aatgctgtct gcctcgtaag
     7141 ggacaaactt taataaccca atccacataa aaacaatcgc aatactcaga cgaattaatg
     7201 tcaggccaat tttatctccc cgacttaaca ggtgcaggta tttttccatt gtgaacatcc
     7261 ttatttatac aatactgctg ttgcggttga attattatca attcgcatcg atgtaacttt
     7321 ccaggatgaa gcattatatt ttctggccat atcagcaatg gcattctcaa gaactgtcgg
     7381 tgatgctgat actgatgaga cgctgatatg tttcagaatt attttatcat ctgcattggt
     7441 ggaaaatgcc ataacgccag ataatagtgt tgcaaataaa acagattttt taaacatata
     7501 aaataccatc aatgaagtga ttaagaagtt ataaagtaac agggaattaa taagattctt
     7561 ttctctgaga cgccagaata tttgttctgg cgtctgattt tgagtttatt tgactaatga
     7621 aaatagatca ttgagtgatt cgctcatcga cggatgagta aatatctgat cgcgtaatat
     7681 gctataaggc agcccggcat ccatcaccat tttcactata ttgatcatct cgtgggagtc
     7741 aacacacagc agtgatgccc ctaatatacg ttgggtttta ttatcaacaa tcgcttttaa
     7801 taccccacga gtatcattca tcactctggc acgcggaatt gcagctacag gcaatgtcac
     7861 cacctgaata tcagcaccac tctctctggc ccgttcttct gtcataccaa ccctggacag
     7921 tggcggtgtc atgaatacgg aataaggcac atttttccga tcatcagtac tacgtctgcc
     7981 ttctcccagc aattcatcac gcacaatgcg gtaatcatcc agtgaaatgt aagtaaattg
     8041 cagcccgccg gtaacatctc ccatcgccca aatattgttt gcggtggtat gtaattgctt
     8101 gtcaacgaca attgccccgc gctcgtttac tgcgatacct gcattttctg gatgtaacga
     8161 agcggtagcc ggttgacgac cggaagctat taacagtgca tccaccgcca gttgggcgtg
     8221 ctcgctatgc acttgcactt gattttcatg gtgactgatt cgctccacat gggcattgag
     8281 gattatatcg acgccctgat cgcgtaaaat cgtcgcgata ttatcagcaa tatcccgatc
     8341 ttcccgaggc aaaaacagcg aagctgcctc taaaatggtt actttgctgc caaaattagc
     8401 gaacatagag gcgaactcaa cgccaatata tccgccgccc aaaataccta aatgcccagg
     8461 caattctttt agattaagta atccggtgct gtcatataca ccaggcgtgg tggtaattcc
     8521 aggaattggc ggaaccacgg cttgtgcacc ggtattaata aaaattttct cgccatgaat
     8581 ctccagattt cccccaggcc gatgaacacg caggctatga ttattgataa actccgcctg
     8641 gccgtcgatc acgtcgatat tgggcatatc cgcaagatta tgaaaattct tattacgtaa
     8701 aaaattaacc acttcatttt tacgctgtat ggcacggaca aaatctgtgt gctgctgtgc
     8761 gtcatgaacc aatgttttgg ttgggataca gccgatatta atacaggtcc cgccatacat
     8821 tgcatttgat tgttcgataa gagccacacg ccaacctgct tttgccagcg tgacggctaa
     8881 tgtttttcca gccttgccaa aaccaataat aactgcctga tatttattca tgatgatttc
     8941 ctgtcgaaac gcgttcattt gacatcatga ctatagacgc aaaagagaaa ataacatttc
     9001 ttcacaacgc ttgatatttt gtcttatcgt ctggtatttt ctttttctta atc
//
LOCUS       DADSYM010000168         9041 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2415
            SAMN08039579-rid11723173.denovo.170, whole genome shotgun sequence.
ACCESSION   DADSYM010000168 DADSYM010000000
VERSION     DADSYM010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039579
            Sequence Read Archive: SRR6362533
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9041)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9041)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980162.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 03:39:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,484
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 4,347
            CDSs (with protein)               :: 4,347
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 43 of 85
            Pseudo Genes (incomplete)         :: 37 of 85
            Pseudo Genes (internal stop)      :: 19 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9041
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039579-rid11723173.denovo.170"
                     /strain="LGE2415"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8302 N 95.9628 W"
                     /collection_date="2015-08-24"
     gene            complement(31..1395)
                     /gene="tdcG"
                     /locus_tag="J1Y83_003809"
     CDS             complement(31..1395)
                     /gene="tdcG"
                     /locus_tag="J1Y83_003809"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HBA7631914.1"
                     /translation="MISAFDIFKIGIGPSSSHTVGPMNAGKSFIDRLESSGLLTATSH
                     IVVDLYGSLSLTGKGHATDVAIIMGLAGNSPQDVVIDEIPAFIELVTRSGRLPVASGA
                     HIVDFPVAKSIIFHPEMLPRHENGMRITAWKGQEELLSKTYYSVGGGFIVEEEHFGLS
                     HDVETPVPYDFHSAGELLKMCDYNGLSISGLMMHNELALRSKAEIDAGFARIWQVMHD
                     GIERGMNTEGVLPGPLNVPRRAVALRRQLVSSDNISNDPMNVIDWINMYALAVSEENA
                     AGGRVVTAPTNGACGIIPAVLAYYDKFRRPVNERSIARYFLAAGAIGALYKMNASISG
                     AEVGCQGEIGVACSMAAAGLTELLGGSPAQVCNAAEIAMEHNLGLTCDPVAGQVQIPC
                     IERNAINAVKAVNAARMAMRRTSAPRVSLDKVIETMYETGKDMNDKYRETSRGGLAIK
                     VVCG"
     gene            complement(1467..1856)
                     /locus_tag="J1Y83_003810"
     CDS             complement(1467..1856)
                     /locus_tag="J1Y83_003810"
                     /EC_number="3.5.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312020.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enamine/imine deaminase"
                     /protein_id="HBA7631915.1"
                     /translation="MKKIIETQRAPGAIGPYVQGVDLGSMVFTSGQIPVCPQTGEIPA
                     DVQDQARLSLENVKAIVVAAGLSVGDIIKMTVFITDLNDFATINEVYKQFFDEHQATY
                     PTRSCVQVARLPKDVKLEIEAIAVRSA"
     gene            complement(1870..4164)
                     /gene="tdcE"
                     /locus_tag="J1Y83_003811"
     CDS             complement(1870..4164)
                     /gene="tdcE"
                     /locus_tag="J1Y83_003811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-ketobutyrate formate-lyase/pyruvate
                     formate-lyase"
                     /protein_id="HBA7631916.1"
                     /translation="MKVDIDTSDKLYADAWLGFKGTDWKNEINVRDFIQHNYTPYEGD
                     ESFLAEATPATTELWEKVMEGIRIENATHAPLDFDTNIATTITAHDAGYIDQPLEKIV
                     GLQTDAPLKRALHPFGGINMIRSSFHAYGREMDSEFEYIFTDLRKTHNQGVFDVYSPD
                     MLRCRKSGVLTGLPDGYGRGRIIGDYRRVALYGISYLVRERELQFADLQSRLEKGEDL
                     EATIRLREELAEHRRALLQIQEMAAKYGFDISRPAQNAQEAVQWLYFAYLAAVKSQNG
                     GAMSLGRTASFLDIYIERDFKAGVLNEQQAQELIDHFIMKIRMVRFLRTPEFDSLFSG
                     DPIWATEVIGGMGLDGRTLVTKNSFRYLHTLHTMGPAPEPNLTILWSEALPIAFKKYA
                     AQVSIVTSSLQYENDDLMRTDFNSDDYAIACCVSPMVIGKQMQFFGARANLAKTLLYA
                     INGGMDEKLKIQVGPKTAPLMDDVLDYDKVMDSLDHFMDWLAVQYISALNIIHYMHDK
                     YSYEASLMALHDRDVYRTMACGIAGLSVATDSLSAIKYARVKPIRDENGLAVDFEIDG
                     EYPQYGNNDERVDSIACDLVERFMKKIKALPTYRNAVPTQSILTITSNVVYGQKTGNT
                     PDGRRAGTPFAPGANPMHGRDRKGAVASLTSVAKLPFTYAKDGISYTFSIVPAALGKE
                     DPIRKTNLVGLLDGYFHHEADVEGGQHLNVNVMNREMLLDAIEHPEKYPNLTIRVSGY
                     AVRFNALTREQQQDVISRTFTQAL"
     gene            complement(4198..5406)
                     /gene="tdcD"
                     /locus_tag="J1Y83_003812"
     CDS             complement(4198..5406)
                     /gene="tdcD"
                     /locus_tag="J1Y83_003812"
                     /EC_number="2.7.2.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312022.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propionate kinase"
                     /protein_id="HBA7631917.1"
                     /translation="MNEFPVVLVINCGSSSIKFSVLNASDCEVLMSGIADGINSENAF
                     LSVNGGEPAPLAHHSYEGALKAIAFELEKRNLNDNVALIGHRIAHGGSIFTESAIITD
                     EVIDNIRRVSPLAPLHNYANLSGIESAQQLFPGVTQVAVFDTSFHQTMAPEAYLYGLP
                     WKYYEELGVRRYGFHGTSHRYVSQRAHSLLNLAEDDSGLVVAHLGNGASICAVRNGQS
                     VDTSMGMTPLEGLMMGTRSGDVDFGAMSWVASQTNQSLGDLERVVNKESGLLGISGLS
                     SDLRVLEKAWHEGHERAQLAIKTFVHRIARHIAGHAASLHRLDGIIFTGGIGENSSLI
                     RRLVMEHLAVLGVEIDTEMNNRSNSFGERIVSSENARVICAVIPTNEEKMIALDAIHL
                     GKVNAPAEFA"
     gene            complement(5432..6763)
                     /gene="tdcC"
                     /locus_tag="J1Y83_003813"
     CDS             complement(5432..6763)
                     /gene="tdcC"
                     /locus_tag="J1Y83_003813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine transporter TdcC"
                     /protein_id="HBA7631918.1"
                     /translation="MSTSDSIVSSQTKQSSWRKSDTTWTLGLFGTAIGAGVLFFPIRA
                     GFGGLIPILLMLVLAYPIAFYCHRALARLCLSGSNPSGNITETVEEHFGKTGGVVITF
                     LYFFAICPLLWIYGVTITNTFMTFWENQLGFAPLNRGFVALFLLLLMAFVIWFGKDLM
                     VKVMSYLVWPFIASLVLISLSLIPYWNSAVIDQVDLGSLSLTGHDGILITVWLGISIM
                     VFSFNFSPIVSSFVVSKREEYEKDFGRDFTERKCSQIISRASMLMVAVVMFFAFSCLF
                     TLSPANMAEAKAQNIPVLSYLANHFASMTGTKTTFAITLEYAASIIALVAIFKSFFGH
                     YLGTLEGLNGLILKFGYKGDKTKVSLGKLNTISMIFIMGSTWVVAYANPNILDLIEAM
                     GAPIIASLLCLLPMYAIRKAPSLAKYRGRLDNVFVTVIGLLTILNIVYKLF"
     gene            complement(6785..7774)
                     /gene="tdcB"
                     /locus_tag="J1Y83_003814"
     CDS             complement(6785..7774)
                     /gene="tdcB"
                     /locus_tag="J1Y83_003814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional threonine ammonia-lyase/L-serine
                     ammonia-lyase TdcB"
                     /protein_id="HBA7631919.1"
                     /translation="MHITYDLPVAIDDIIEAKQRLAGRIYKTGMPRSNYFSERCKGEI
                     FLKFENMQRTGSFKIRGAFNKLSSLTDAEKRKGVVACSAGNHAQGVSLSCAMLGIDGK
                     VVMPKGAPKSKVAATCDYSAEVVLHGDNFNDTIAKVSEIVEMEGRIFIPPYDDPKVIA
                     GQGTIGLEIMEDLYDVDNVIVPIGGGGLIAGIAVAIKSINPTIRVIGVQSENVHGMAA
                     SFHSGEITTHRTTGTLADGCDVSRPGNLTYEIVRELVDDIVLVSEDEIRNSMIALIQR
                     NKVVTEGAGALACAALLSGKLDQYIQNRKTVSIISGGNIDLSRVSQITGFVDA"
     gene            complement(7873..8811)
                     /gene="tdcA"
                     /locus_tag="J1Y83_003815"
     CDS             complement(7873..8811)
                     /gene="tdcA"
                     /locus_tag="J1Y83_003815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001683315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator TdcA"
                     /protein_id="HBA7631920.1"
                     /translation="MSTILLPKTQHLVVFQEVIRSGSIGSAAKELGLTQPAVSKIIND
                     IEDYFGVELVVRKNTGVTLTPAGQLLLSRSESITREMKNMVNEISGMSSEAVVEVSFG
                     FPSLIGFTFMSGMINKFKEVFPKAQVSMYEAQLSSFLPAIRDGRLDFAIGTLSAEMKL
                     QDLHVEPLFESEFVLVASKSRTCTGTTTLESLKNEQWVLPQTNMGYYSELLTTLQRNG
                     ISIENIVKTDSVVTIYNLVLNADFLTVIPCDMTSPFGSNQFITIPVEETLPVAQYAAV
                     WSKNYRIKKAASVLVELAKEYSSYNGCRRRQLIEVD"
BASE COUNT         2385 a         2347 c         2082 g         2227 t
ORIGIN      
        1 tgcacccaag gatgatagct gacagcaatg tcagccgcag accactttaa tagccagtcc
       61 tccgcgtgat gtttcgcggt atttatcgtt catatctttg ccggtttcat acatcgtctc
      121 gatcacttta tcgagtgaaa cacgcggtgc cgaggtgcgg cgcattgcca tccgcgcggc
      181 gtttactgct ttcacggcat taatggcatt acgttcaatg cacgggattt gtacctgtcc
      241 ggcaaccgga tcgcaggtca gcccaaggtt atgctccatc gcgatttccg ccgcattgca
      301 tacctgcgcc ggactgccgc ccagtagttc agttaaccct gccgccgcca ttgaacaggc
      361 tacaccaatc tccccctgac agccgacttc cgcgccagag atggaggcgt tcattttata
      421 cagcgcgcca atagcccccg ctgccagaaa atagcgggca attgaccgct cgtttaccgg
      481 acgacggaac ttatcgtaat aagccagcac tgccggaata atgccgcacg caccgttagt
      541 cggtgccgtt accacgcgcc cgccagctgc gttttcttca ctaaccgcta gcgcgtacat
      601 gttgatccag tcgatgacat tcatcggatc gttagagatg ttatcgctgg aaaccagttg
      661 acgacgcagc gctacggcac ggcgcggcac attgagcgga ccaggcagca cgccttcagt
      721 attcattcca cgttcaatac cgtcatgcat cacttgccag atacgggcaa accctgcgtc
      781 aatttccgct ttactgcgca gcgccagctc gttgtgcatc atcaggccag atatagacag
      841 gccgttgtaa tcacacattt tcagcagttc acctgctgag tggaaatcgt aaggtacggg
      901 tgtttcgaca tcgtgcgaca ggccgaagtg ttcttcttcg acaataaacc cgccgccgac
      961 agagtaatag gttttgctta atagttcttc ctgtcccttc caggcagtga tccgcattcc
     1021 gttctcatgg cgaggcaaca tttcgggatg gaagataatg ctctttgcta caggaaaatc
     1081 aacaatatgc gcgccagaag ccaccggcaa ccgcccgctg cgcgtcacta actctataaa
     1141 tgcagggatc tcatcaatga caacatcctg cggactgttt cctgccagtc ccatgatgat
     1201 ggcgacatcc gtggcatggc ctttgcccgt cagtgacaac gacccgtaca gatcgaccac
     1261 aatatggctc gtcgcggtta ataagccgct actttccagc cgatcaataa aactttttcc
     1321 ggcattcatt ggccccacag tatgcgaact ggagggacca atcccaattt tgaaaatatc
     1381 gaatgcacta atcatatcca caccctcgga ttgccgttca gtaaagtgga gcggaacgac
     1441 cttgcgaccg tcccgctcac gaggccttac gcactacgta ctgcgatggc ttcaatttcc
     1501 aacttcacat ctttcggcaa acgcgcgacc tgcacacagc tccgggtcgg gtaggtcgcc
     1561 tgatgttcat cgaagaactg cttatagact tcattgatgg tggcaaaatc attcagatcg
     1621 gtgatgaaca cggtcatctt gatgatatcg cccacactca gcccggcagc aaccacgatc
     1681 gctttgacgt tttcgaggct taaacgcgcc tgatcttgca catcagccgg gatctcaccg
     1741 gtctgtgggc aaaccggaat ttgcccggag gtgaagacca tgctgcctaa atcaacgccc
     1801 tgaacataag ggccgattgc gcctggggca cgttgcgttt cgataatctt tttcatacat
     1861 cctccggcgt cagagcgcct gggtaaaggt acgtgaaata acatcctgtt gctgttcacg
     1921 ggtcagtgcg ttaaagcgca cggcgtagcc agagacacgg attgtcaggt taggatattt
     1981 ttccgggtgc tcgatggcat ccagcagcat ttcccgattc attacgttga cgttgaggtg
     2041 ttgaccgcct tcgacatccg cttcgtggtg gaaataccca tccagcaggc cgacaaggtt
     2101 cgttttacgt atcggatctt ctttgcccag cgccgcaggg acgatggaga aggtgtacga
     2161 gatcccatct ttggcgtagg tgaacggcag tttcgccacc gacgtcaatg aggccacggc
     2221 gcctttgcga tcacgaccgt gcatcgggtt agcgcccggc gcgaacggtg ttccggcgcg
     2281 gcgaccgtcc ggcgtgttcc cggttttctg accgtacacc acgttagagg tgatagtcag
     2341 aatcgattgg gtcggcacag cattgcgata ggttggcaaa gctttaattt tcttcataaa
     2401 gcgttcaacc agatcgcagg caatgctgtc tacgcgctcg tcgttgttgc catactgcgg
     2461 atattcgccg tcaatctcga aatccaccgc caggccgttt tcatcacgga ttggtttcac
     2521 acgagcatat ttgatggcag acagggagtc cgttgccacc gacaggcccg cgatgccgca
     2581 cgccatggtg cgatagacat cacgatcatg tagcgccatc agcgaagctt cgtagctgta
     2641 cttgtcgtgc atgtagtgaa tgatattcag cgcgctgatg tactgtaccg ccagccagtc
     2701 catgaagtgg tcgaggctat ccatcacttt gtcgtagtcc agcacgtcat ccatcaacgg
     2761 agccgttttc gggccaacct gaatcttcag cttctcgtcc attccgccgt tgattgcgta
     2821 cagtagcgtt ttcgccaggt tagcgcgtgc tccaaagaac tgcatttgct taccaatcac
     2881 cattgggctg acgcaacagg caatcgcgta atcgtcgcta ttgaagtcag tacgcatcag
     2941 atcgtcgttt tcatactgca aagaggatgt gacgatagac acctgcgcgg catatttttt
     3001 gaaggcaatc ggcaaggctt ccgaccacag aatagtcagg ttaggttccg gtgcaggccc
     3061 catagtgtgc agggtgtgca aatagcggaa ggagtttttg gtcaccaggg ttcgaccgtc
     3121 cagccccatc ccgccgatca cttccgtcgc ccagatcggg tcgccggaga acagcgaatc
     3181 aaattccggc gtacgcaaga agcgcaccat acggatcttc atgatgaagt ggtcgatcag
     3241 ttcctgtgcc tgctgctcat tgagtacgcc tgctttaaag tcgcgctcaa tgtagatatc
     3301 gaggaacgat gccgtgcgcc ccagcgacat cgcgccgcca ttttgcgatt tcactgccgc
     3361 caggtaagcg aagtagagcc actgcaccgc ttcctgtgca ttctgcgccg gacgggagat
     3421 gtcaaaaccg tatttcgccg ccatttcttg aatctgcaac agcgcacgac gatgttccgc
     3481 cagctcctca cgcagacgga tcgtagcttc gagatcttcg cctttttcca gacgggactg
     3541 gagatcggca aactgcagtt cgcgttcacg taccagataa ctgatgccgt aaagcgccac
     3601 gcggcggtag tcaccgatga tacgcccacg accataacca tctggtaaac cggtcagtac
     3661 accagattta cggcagcgca gcatatccgg tgagtaaaca tcaaatacgc cctggttatg
     3721 ggttttacgc agatcggtaa agatgtattc aaattcgcta tccatttcac gaccatacgc
     3781 gtggaatgaa cttctaatca tattaatgcc gccgaacgga tgcagcgcac gctttaatgg
     3841 cgcatccgtt tgcagaccca ctattttttc cagcggctgg tcaatatatc ctgcatcatg
     3901 agcggtaatt gtggtggcaa tattggtatc gaaatccaat ggcgcgtgag tcgcattttc
     3961 gatacggatg ccttccatga ctttttccca caattccgtg gtggcaggcg tcgcttcggc
     4021 gagaaaagat tcatcgcctt cataaggtgt atagttatgt tgaataaaat cgcggacatt
     4081 aatttcgttt ttccagtccg tacctttaaa gccaagccat gcgtcggcgt acagcttatc
     4141 gctggtatca atatctacct tcatgaaaaa taatctctct acaatacttc aactaaatta
     4201 tgcaaattct gcgggcgcgt tcactttgcc taaatgaatg gcatccaaag caatcatttt
     4261 ttcttcgtta gtcggaatta cggcgcaaat gacacgcgca ttttcactgg aaacaattcg
     4321 ctctccaaaa gagttagagc gattattcat ttctgtatca atttctacgc ctaatacagc
     4381 taaatgttcc atgaccagac gacgaattaa acttgaattc tctcctattc cgccggtgaa
     4441 tataattcca tccaggcgat gtaatgaagc agcgtgtccg gcaatatgac gggcaattcg
     4501 gtgaacaaag gttttaattg ccagttgcgc gcgttcgtga ccttcatgcc aggctttttc
     4561 cagaacacgt aaatccgaag aaaggccgga aatacctaat aatcccgact ctttattcac
     4621 tacgcgttcc aggtccccca ggctctggtt ggtttggctg gcgacccagg acatcgcccc
     4681 gaagtcgaca tcaccactgc gggtacccat catcaagcct tccagcggcg tcattcccat
     4741 ggaagtgtca acactctgac cgttgcgaac cgcgcagatt gacgcgccat tgccaagatg
     4801 cgccacaacc aggccggagt catcttccgc cagattcagc agagaatgcg cgcgctggga
     4861 aacatagcgg tgcgacgtgc cgtggaaacc ataacgacgt acacctaact cttcataata
     4921 tttccacggc aggccgtata aataagcttc cggagccatc gtctggtgga aactggtatc
     4981 aaataccgcc acctgagtta cgcccgggaa taattgctgc gccgattcaa taccacttaa
     5041 attggcgtaa ttatgcaggg gtgccagtgg agaaacgcga cggatattat caatgacttc
     5101 atcggtaata atggcggact cggtaaaaat actgccgccg tgagcgatgc ggtggccaat
     5161 taaggccaca ttgtcattta aattccgttt ttccagttca aatgcaattg ccttcaatgc
     5221 accttcgtag ctgtggtgag ccagcggtgc tggctctccc ccatttacgg ataagaatgc
     5281 attttccgag ttaataccgt cggcaatacc tgacattaat acttcacagt cgctggcatt
     5341 gagtacggaa aacttaatcg aagacgaacc acagttaata accaaaacaa ccggaaattc
     5401 attcatctct tttctcatcc tgagttacgg attaaaacag tttgtatacg atgttcagga
     5461 tggtcagcag accaatcacg gtaacaaaca cgttatccag acgaccacgg tatttcgcca
     5521 gagacggcgc tttacggatg gcatacatcg gcaacaggca cagcagtgat gcgataatcg
     5581 gtgcgcccat ggcttcaatc aggtcgagga tgttcgggtt ggcgtaggca acaacccagg
     5641 tggagcccat aatgaaaatc atgctgatag tattcagttt acccagcgac actttggttt
     5701 tgtcaccttt ataaccgaac ttcagaatca aaccattcag cccttccagc gtccccagat
     5761 agtgaccgaa gaaagatttg aaaatagcca cgagtgcgat gatggaagcg gcatattcca
     5821 gtgtaatcgc aaacgttgtt ttggtaccgg tcatggacgc aaagtggtta gccagataag
     5881 aaagcactgg aatattctgc gctttcgctt cagccatatt cgccggagac agcgtaaaca
     5941 ggcagctaaa ggcaaagaac atcaccactg caaccatcag catactggcg cgagagatga
     6001 tttgtgaaca tttacgctcg gtgaagtcgc gaccaaaatc tttctcgtac tcttcacgtt
     6061 tagaaaccac gaaggaagag acgattggcg agaagttaaa ggagaaaacc atgatggaaa
     6121 tccccagcca gacggtgatc aggataccgt catgaccagt taacgacagc gaaccgaggt
     6181 caacctggtc gataactgca gagttccagt aagggatcag cgaaagagaa atcagcacca
     6241 ggctggcgat aaacggccat accagatagc tcatcacttt aaccatcaga tccttaccaa
     6301 accagatgac gaaagccatc agcagcaaca ggaacagcgc cacaaagccg cgattcagcg
     6361 gtgcaaagcc gagctggttt tcccagaacg tcataaaggt attggtaata gtaacgccat
     6421 aaatccacaa cagtgggcaa atcgcgaaga agtacaggaa cgtgataacc acgccgccag
     6481 ttttaccaaa atgctcttcc accgtttccg taatgttgcc ggaagggtta gagccagaaa
     6541 gacacagacg cgccagtgcc cggtggcaat aaaacgcgat ggggtatgct aataccaaca
     6601 tcagaagaat cgggatcagt ccgccaaaac ctgcgcggat agggaagaac agcaccccgg
     6661 cgccgattgc cgtaccaaac aagcctaacg tccatgtggt atctgattta cgccaggacg
     6721 attgttttgt ctggctggat acaatgctat ctgaagtact catatcctat cctcaacgaa
     6781 ttaattaagc gtcaacgaaa ccggtgattt gagagacgcg agaaagatcg atattgccgc
     6841 cggaaataat actgacggtt tttctgtttt gaatatattg gtctaattta ccgcttaata
     6901 atgcagcaca tgccagagcg cctgcgcctt cggtgacgac tttattgcgc tgaattaagg
     6961 caatcatact gtttctgatt tcgtcttcgc tgaccagcac gatgtcatcg actaattcac
     7021 gaacgatttc gtaagttaaa ttacccgggc gggagacatc acaaccatcc gccagggtgc
     7081 cggtagttcg gtgcgtagtt atttctccgg agtggaaaga agccgccatg ccgtgaacgt
     7141 tttcagactg tacgccaata acacggatgg tcgggttgat agatttaatt gccaccgcaa
     7201 tacctgcaat taaaccgccg ccgccaattg gcacaatcac gttatcgaca tcatagagat
     7261 cttccataat ttccagacca atcgttccct ggccagcaat cactttcgga tcatcgtaag
     7321 gtgggataaa aatacggcct tccatttcga caatttcgct cactttagca atagtgtcgt
     7381 tgaagttatc accatgcaaa acgacttctg cggagtagtc gcacgttgcc gcgactttgg
     7441 atttcggcgc accttttggc atcaccactt taccgtcaat acccagcatc gcgcaggaga
     7501 gcgaaacccc ttgcgcatgg ttgcccgcag agcaggccac tacaccttta cgtttttccg
     7561 catcagtcag tgaacttaat ttattaaatg cgccacgaat tttaaatgaa ccggtacgct
     7621 gcatgttttc gaatttgagg aatatttcac ctttgcaacg ttcactaaaa tagttggagc
     7681 gaggcatacc tgttttataa attcgcccag ccagtcgttg ttttgcttca atgatgtcat
     7741 caatagcaac cgggagatcg tatgtaatat gcattataaa acctcttagc ctgaattaaa
     7801 tatgtaggta accgtaaccg acacctgcaa aacagacagg tggattattt atgtttaaat
     7861 taaaacaaat aactaatcaa cttcaattaa ttgccttcgt ctacacccat tataagatga
     7921 atactctttg gctaattcca ccaaaaccga tgctgctttt ttaatacgat aatttttcga
     7981 ccataccgcg gcatattgtg ccacaggtaa tgtttcttca accggaatag taataaattg
     8041 attagaacca aaaggtgatg tcatatcaca aggaattaca gttaagaaat cagcattgag
     8101 aacaagatta taaattgtca cgactgagtc ggttttaacg atgttttcaa tactgatgcc
     8161 atttctttgt aacgtagtaa gtagttcgct gtagtacccc atattggttt gtggcaacac
     8221 ccactgttcg ttcttcaacg actccagcgt ggtggtgccg gtgcatgttc gggacttact
     8281 ggctaccagc acaaactcgg actcgaacag cggctcaaca tgtaaatcct gaagcttcat
     8341 ttctgcgctt aacgtaccaa tcgcaaaatc cagtcgaccg tcgcggattg ccggtaagaa
     8401 cgaagacagt tgcgcttcat acatagaaac ctgcgctttc gggaacacct ctttgaactt
     8461 gttgatcatc cctgacataa aagtaaaacc aatcaatgaa ggaaaaccaa atgagacttc
     8521 caccaccgcc tcagaagaca taccgcttat ctcattaacc atatttttca tttcacgggt
     8581 aatggattcg gaacgggaga gtaacaattg accggcaggt gttaatgtta caccggtatt
     8641 tttccgcacc actaactcca caccaaaata atcctcaata tcgttaatga ttttactgac
     8701 ggccggttga gttaacccta actcttttgc agccgagccg atagaaccac ttctaataac
     8761 ttcctgaaag actaccaggt gctgcgtttt cggaagaaga atagtgctca taacgaccta
     8821 cgttaattac ctcattgacg gcatgaagtg tatcaaaatg aaatgaacag gatatgtgcg
     8881 accactcaca aattaacttt caatactttc catggcatcg ttaccccaac caaatacctt
     8941 taaatatcaa caagttaaaa tatgaaaatc agcataaacc ctgatttttg tcaaaaaaaa
     9001 tgacggggat aaccatattt tatggcgata acatcattcg t
//
LOCUS       DADSYM010000169         8846 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2415
            SAMN08039579-rid11723173.denovo.171, whole genome shotgun sequence.
ACCESSION   DADSYM010000169 DADSYM010000000
VERSION     DADSYM010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039579
            Sequence Read Archive: SRR6362533
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8846)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8846)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980162.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 03:39:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,484
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 4,347
            CDSs (with protein)               :: 4,347
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 43 of 85
            Pseudo Genes (incomplete)         :: 37 of 85
            Pseudo Genes (internal stop)      :: 19 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8846
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039579-rid11723173.denovo.171"
                     /strain="LGE2415"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8302 N 95.9628 W"
                     /collection_date="2015-08-24"
     gene            92..1051
                     /locus_tag="J1Y83_003816"
     CDS             92..1051
                     /locus_tag="J1Y83_003816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A deacylase LpxR family protein"
                     /protein_id="HBA7631921.1"
                     /translation="MKKSVIAGVFIALSFTTCSAIANSLALSLANDDAGKFQPILNDI
                     YGNKHENRDDYSQGLFLGYSHDISDSSQLSLHIAQDIYSPSGSNKRHNTAVTGDRAFS
                     AYTHTGIEWNSLANDWIRYRLGTDIGVVGPDAGGQKVQNKAHEIIGAEKYHAWDDQIE
                     NRYGYTVKGMLSMTPSMDILGANVGLYPEVSAVTGNLFQYVAYGATIAIGNDKTFNSD
                     NGFGLLAPRGLMHMSDTSGFKYKIFAGMERRDVNRNYTLEGKTIQTKQTTVSLNKTVD
                     EYQVGATIGYAPVAFTLAFNKVTSEFKTGDDYSFINGAITFFF"
     gene            complement(1195..1266)
                     /locus_tag="J1Y83_003817"
                     /pseudo
     CDS             complement(1195..1266)
                     /locus_tag="J1Y83_003817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311528.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein Q"
     gene            complement(1263..1928)
                     /locus_tag="J1Y83_003818"
     CDS             complement(1263..1928)
                     /locus_tag="J1Y83_003818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein phosphatase"
                     /protein_id="HBA7631922.1"
                     /translation="MNIYERIDGSKYRNIWVAGDLHGCYTNLMNKLDTIGFDTKKDLL
                     ISVGDLVDRGTENVECLELITFPWFRAVRGNHEQMMIDGLSERGNVNHWLLNGGGWFF
                     NLDYDKETLAKVLAHKAEELPLIIELVSKDKKYVICHADYPCDKYEFGKPVDHQQVIW
                     NRERISNSQDGIVKEIKGADTFIFGHTPAVKPLKFANQMYIDTGAVFCGNLTLIQVQG
                     EGA"
     gene            complement(1925..2536)
                     /locus_tag="J1Y83_003819"
     CDS             complement(1925..2536)
                     /locus_tag="J1Y83_003819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein NinG"
                     /protein_id="HBA7631923.1"
                     /translation="MAKPARRRCKNDECREWFHPAFANQWWCSPECGTKIALERRSKE
                     REKAEKAAEKKRRREEQKQKDKLKIRKLALKPRSYWIKQAQQAVNAFIRERDRDLPCI
                     SCGTLTSAQWDAGHYRTTAAAPQLRFDERNIHKQCVVCNQHKSGNLVPYRVELISRIG
                     QEAVDEIESNHNRHRWTVEECKAIKAEYQQKLKDLRNSRSEAA"
     gene            complement(2529..2699)
                     /locus_tag="J1Y83_003820"
     CDS             complement(2529..2699)
                     /locus_tag="J1Y83_003820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000950944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein ninF"
                     /protein_id="HBA7631924.1"
                     /translation="MIDPNRSYEQESVERALTCANCGQKLHVLEVHVCEHCCAELMSD
                     SNSSMHEEEDDG"
     gene            complement(2696..2878)
                     /locus_tag="J1Y83_003821"
     CDS             complement(2696..2878)
                     /locus_tag="J1Y83_003821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016063117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NinE family protein"
                     /protein_id="HBA7631925.1"
                     /translation="MRRQRRSFTDIICENCKYLPTKRSRNKRKPIPKESDVKTFNYTA
                     HLWDIRWLRHRARNTR"
     gene            complement(2875..3402)
                     /locus_tag="J1Y83_003822"
     CDS             complement(2875..3402)
                     /locus_tag="J1Y83_003822"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage N-6-adenine-methyltransferase"
                     /protein_id="HBA7631926.1"
                     /translation="MTIKSNTPAHDKDCWQTPLWLFDALDIEFGFWLDSAASDKNALC
                     AHWLTEADDALNSEWISHGAIWNNPPYSNIRPWVEKAAEQCIQQRQTVVMLVPEDMSV
                     GWFSKALESVDEVRIITDGRINFIEPSTGLEKKGNSKGSMLLIWRPFISPRRMFTIVS
                     KAALMAIGQGVRRAA"
     gene            complement(3399..3839)
                     /locus_tag="J1Y83_003823"
     CDS             complement(3399..3839)
                     /locus_tag="J1Y83_003823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001513013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein NinB"
                     /protein_id="HBA7631927.1"
                     /translation="MKKLTFEIRSPAHQQNAIHAVQQILPDPTKPIVVTIQERNRSLD
                     QNRKLWACLGDVSRQVEWHGRWLDAESWKCVFTAALKQQDVVPNLAGNGFVVIGQPTS
                     RMRVSEFAELLELIQAFGTERGVKWSDEARLALEWKARWGDRAA"
     gene            complement(3913..4203)
                     /locus_tag="J1Y83_003824"
     CDS             complement(3913..4203)
                     /locus_tag="J1Y83_003824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein ren"
                     /protein_id="HBA7631928.1"
                     /translation="MTGKEAIIHYLGTHNSFCAPDVAALTGATVTSINQAAAKMARAG
                     LLVIEGKVWRTVYYRFATREEREGKMSTNLIFKECRQSAAMKRVLAVYGVKR"
     gene            complement(4200..4901)
                     /locus_tag="J1Y83_003825"
     CDS             complement(4200..4901)
                     /locus_tag="J1Y83_003825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Replication protein P"
                     /protein_id="HBA7631929.1"
                     /translation="MKNIAAQMVNFDREQMRRIANNMPEQYDEKPQVQQVAQIINGVF
                     SQLLATFPASLANRDQNELNEIRRQWVLAFRENGITTMEQVNAGMRVARRQNRPFLPS
                     PGQFVAWCREEASVIAGLPNVSELVDMVYEYCRKRGLYPDAESYPWKSNAHYWLVTNL
                     YQNMRANALTDAELRRKAADELSCMTARINRGEAIPEPVKQLPVMGGRPLNRAQALAK
                     IAEIKAKFGLKGASV"
     gene            complement(4898..5797)
                     /locus_tag="J1Y83_003826"
     CDS             complement(4898..5797)
                     /locus_tag="J1Y83_003826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HBA7631930.1"
                     /translation="MTNTAKILNFGRGNFAGQERNVADLDDGYARLSNMLLEAYSGAD
                     LTKRQFKVLLAILRKTYGWNKPMDRITDSQLSEITKLPVKRCNEAKLELVRMNIIKQQ
                     GGMFGPNKNISEWCIPQNEGKSPKTRDKTSLKLGDCYPSKQGNTKDTNTKENRKDYSS
                     ENSGESSDQPENDLSVVKPDAAIQSGSKWGTAEDLTAAEWMFDMVKTIAPSARKPNFA
                     GWANDIRLMRERDGRNHRDMCVLFRWACQDNFWSGNVLSPAKLRDKWTQLEINRNKQQ
                     AVVTASKPKLDLTNTDWIYGVDL"
     gene            complement(5830..6123)
                     /locus_tag="J1Y83_003827"
     CDS             complement(5830..6123)
                     /locus_tag="J1Y83_003827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA7631931.1"
                     /translation="MVRANKRNEALRIESALLNKIAMLGTEKTAEAVGVDKSQISRWK
                     RDWIPKFSMLLAVLEWGVVDDDMARLARQVASILTNKKRPAATERSEQIQMEF"
     gene            complement(6243..6458)
                     /locus_tag="J1Y83_003828"
     CDS             complement(6243..6458)
                     /locus_tag="J1Y83_003828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001194218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBA7631932.1"
                     /translation="MQTLSERLKKRRIALKMTQTELATKAGVKQQSIQLIEAGVTKRP
                     RFLFEIAMALNCDPVWLQYGTKRGKAA"
     gene            6562..7194
                     /locus_tag="J1Y83_003829"
     CDS             6562..7194
                     /locus_tag="J1Y83_003829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000028393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LexA family transcriptional regulator"
                     /protein_id="HBA7631933.1"
                     /translation="MSISSRVKSKRIQLGLNQAELAQKVGTTQQSIEQLENGKTKRPR
                     FLPELASALGVSVDWLLNGTSDSNVRFVGHVEPKGKYPLISMVRAGSWCEACEPYDIK
                     DIDEWYDSDVNLLGNGFWLKVEGDSMTSPVGQSIPEGHMVLVDTGREPVNGSLVVAKL
                     TDANEATFKKLVIDGGQKYLKGLNPSWPMTPINGNCKIIGVVVEARVKFV"
     gene            7191..7595
                     /locus_tag="J1Y83_003830"
     CDS             7191..7595
                     /locus_tag="J1Y83_003830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000618033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA7631934.1"
                     /translation="MIRIAALLSILLTTSANSECWIVTNLHGYGAMNGDRYEFTKDST
                     EDSVFNVTINGDKSSVYESVSGVYPEMKYTALSSNTMVGEYQSGGGITVETWSITTDK
                     KALYSKVMNIPGMQQLTSTKSFVGDVVGTCNQ"
     gene            7815..8270
                     /locus_tag="J1Y83_003831"
     CDS             7815..8270
                     /locus_tag="J1Y83_003831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001370778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HBA7631935.1"
                     /translation="MCDTKRSIGRKCESGLAANVPLRGVFVQDYDSHTQPKLTDRRIQ
                     MDAQTRRRERRAEKQAQWKAANPLLVGVSAKPVNRPILSLNRKPKSRVESALNPIDLT
                     VLAEYHEQIESNLQRIERKNHRVWYSKPGEFGITCQGRQKVKGESIPLA"
     gene            8267..>8846
                     /locus_tag="J1Y83_003832"
     CDS             8267..>8846
                     /locus_tag="J1Y83_003832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001271095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA7631936.1"
                     /translation="MRYVMKKIDYKSIPKPIDSASERKKHKKEAEKLANYISFIRNNA
                     HGDGDKKLLSDARTQAFGILRKQMQYRLHPGYIIEIRPTERQLFLLNSVFDFVNVVGD
                     LIDRSVDKDPDTNSFLLTNKEYLYGKFGINGWQKYVRFLRAFVDAYKNSDMIYTYLPG
                     GDNCTLSADNRIFIPVLGLGLINAVNEKDVIIV"
BASE COUNT         2274 a         2085 c         1956 g         2531 t
ORIGIN      
        1 tataaaacat catattgatt acgcaccttg tattacaaac tcatgtatgt aaaatacgcg
       61 caccattcaa aaaaaaggaa gacaataaca tatgaaaaaa agtgtcatcg ctggcgtctt
      121 tattgctctg tcatttacca cgtgttcagc tatcgcgaac agccttgcat tatcattagc
      181 aaatgatgat gcagggaagt ttcaaccaat acttaatgat atttatggca ataaacatga
      241 aaacagagat gattactcac aaggcttatt tctgggatat agccacgata tctcagactc
      301 gagccaatta tctctccata ttgcgcaaga tatttactct ccatcaggca gtaataaaag
      361 acacaacaca gctgtaactg gagacagagc ttttagtgca tacactcaca ctggtattga
      421 atggaactcc cttgcgaatg actggattcg ctatcgatta ggtactgaca taggtgttgt
      481 tggccccgac gcaggcggtc agaaagtaca aaataaagct catgagatta ttggggcaga
      541 aaaatatcat gcatgggatg atcaaataga gaatcgctac ggttatactg taaaagggat
      601 gctatccatg acaccaagta tggatatttt aggtgctaat gttggattat accctgaagt
      661 ttctgctgtt actggaaact tatttcaata tgtagcatat ggcgcaacca ttgccattgg
      721 taatgataaa accttcaatt cggataatgg ctttggtctg ctggctcccc gtggtttaat
      781 gcatatgtcc gatacaagcg gattcaaata caagattttt gcaggtatgg aaagacgaga
      841 tgtcaatcgc aactatactc tcgaaggaaa aacaatacag acgaaacaaa caacagtatc
      901 gctaaacaaa actgttgatg aatatcaagt tggcgcaaca attgggtatg cacctgtagc
      961 cttcacacta gcatttaata aagtaacatc agaattcaag acaggggatg actattcatt
     1021 tataaatgga gcaatcacct tcttttttta actgaattga attcaatcaa aataacataa
     1081 gtccaacaaa aacataaagt gcgaaatgaa tgccagctcc atttatttcg cactataaaa
     1141 gattaaaagt tgcaataaaa taataaaatg actcagttac gaaaaccaat aaactgtggc
     1201 cagtagtgag tcgctcatca tcgggctttt tggcgaatga aatttagcta cgctttcgag
     1261 tctcatgcgc cttctccctg tacctgaatc aatgtgaggt ttccgcagaa cactgcgcca
     1321 gtatcgatat acatctggtt ggcaaatttg agtggtttca ctgctggcgt atgaccaaag
     1381 atgaacgtgt ccgcgccttt gatttctttc acgatcccgt cttgtgagtt gctgattcgt
     1441 tcgcggttcc agattacctg ctgatgatca actggctttc caaactcgta tttatcacaa
     1501 ggataatcgg cgtggcaaat gacatatttt ttatctttgc tcaccagttc gatgattaac
     1561 ggaagttctt ctgctttatg ggcaagaact ttagccagag tctctttgtc gtaatcgaga
     1621 ttaaagaacc agccaccgcc attaagcagc cagtgattaa cgtttccacg ctctgataag
     1681 ccatcaatca tcatttgctc atggtttcca cgcacagctc tgaaccaggg gaatgtgatt
     1741 aattccagac attcgacgtt ctctgtaccg cgatcaacca aatcgccaac cgagatgagc
     1801 aggtcttttt tggtgtcgaa tccaatcgta tccagtttgt tcatcaggtt cgtgtagcat
     1861 ccgtgcaggt cgccagctac ccaaatattt cggtatttgc tgccatcaat tctttcgtag
     1921 atattcatgc agcctcactt ctgctgtttc gcaggtcttt gagtttctgt tggtactctg
     1981 ccttgatcgc cttgcactct tcgacagtcc agcgatggcg gttatggttt gattcgattt
     2041 cgtctactgc ttcctgcccg atgcggctaa tcagttcgac gcgatacgga acgagatttc
     2101 cgcttttgtg ctggttgcac accacgcatt gcttgtgaat attgcgttca tcaaatcgga
     2161 gttgaggtgc cgcagcagtt gtccggtaat gtccggcatc ccactgagcc gacgtgagcg
     2221 ttccgcacga gatacatggt aagtcgcggt ctctttctct gatgaaggcg tttacggctt
     2281 gttgggcttg tttaatccag taactgcggg gctttaaggc gagttttcga atcttaagtt
     2341 tatctttctg tttctgctcc tctcgtcgtc gtttcttctc tgctgctttt tccgcttttt
     2401 cgcgttcttt acttcgtcgt tcgagtgcta tcttggttcc acactctgga gagcaccacc
     2461 actgattagc gaatgcaggg tgaaaccatt cccgacattc atcgttttta catcgtcttc
     2521 gcgctggttt agccatcatc ttcttcctcg tgcatcgagc tattcgaatc gctcatcagc
     2581 tctgcacagc agtgctcaca cacgtgaact tccagcacat gcagcttctg accgcaatta
     2641 gcgcacgtta aagcccgctc gacgctttct tgttcgtaac ttcgatttgg gtcaatcacc
     2701 ttgtattcct cgcacgatgt cttagccacc ggatatccca caggtgagcc gtgtagttga
     2761 aggtttttac gtcagattct tttgggattg gcttgcgttt atttctggag cgtttcgttg
     2821 gaaggtattt gcagttttcg cagatgatgt cggtgaaact tcgtcgttgt cgcctcatgc
     2881 cgccctcctg acgccctgcc cgatcgccat caatgccgct ttggatacga tagtaaacat
     2941 ccgtcgagga ctaatgaacg gtcgccaaat cagcagcatg gagcctttgc tgtttccctt
     3001 cttctccagc cctgtcgatg gttcgataaa attaatccgt ccatcagtga taatacgaac
     3061 ttcgtcaaca ctctccagag ccttgctgaa ccatccgaca gacatatcct ctggcacaag
     3121 catcactacc gtctgtcgct gttgtatgca ctgctcagcg gctttttcca cccacggcct
     3181 gatattgctg tacggtgggt tattccagat tgcaccgtgg cttatccact cagaatttag
     3241 cgcgtcgtca gcctcagtta gccagtgagc gcacagagcg tttttgtcgc tcgctgccga
     3301 atccagccag aatccaaact caatatccag tgcatcaaaa agccaaagcg gcgtttgcca
     3361 gcagtccttg tcgtgtgctg gcgtatttga tttgatagtc atgcagcccg atctccccat
     3421 cgcgctttcc attcgagagc cagtcgcgct tcgtctgacc acttaacgcc acgctctgta
     3481 ccgaatgcct gtataagctc taatagctcc gcaaattcgc ttacacgcat cctgctggtt
     3541 ggctggccta ttaccacaaa gccattcccg gcaaggttag gaacaacatc ctgctgcttt
     3601 aatgctgcgg taaacacaca cttccagctt tctgcatcca gccagcgacc atgccattca
     3661 acctgacgag agacgtcacc taagcaggcc catagcttcc tgttttggtc taagctgcgg
     3721 ttgcgttcct gaatggttac tacgattggt ttggttgggt ctggaaggat ttgctgtact
     3781 gcgtgaatag cgttttgctg atgtgctgga gatcgaattt caaaggttag ttttttcatg
     3841 acttccctct cccccaaata aaaaggcctg cgattaccag caggcctgtt attagctcag
     3901 tgatgtagat ggtcatcttt taactccata taccgccaat acccgtttca tcgcggcact
     3961 ctggcgacac tccttaaaaa tcaggttcgt gctcatcttt ccttcccgtt cttccctagt
     4021 agcgaaccgg taatacaccg ttcgccagac cttaccttcg ataaccagaa gacctgcccg
     4081 tgccatttta gccgcggcct gatttatgct ggttactgtt gcgcctgtta acgcggcaac
     4141 gtccggcgca cagaagctat tatgcgtccc caggtaatga ataattgcct ctttgcccgt
     4201 catacacttg ctcctttcag cccaaactta gctttgattt ctgcgatctt cgccagagcc
     4261 tgtgcacgat ttagaggtct accgcccatg acaggaagtt gttttactgg ttcaggtata
     4321 gcctcaccac ggttaattcg tgcggtcata caggacagtt catcggcagc cttgcgccgt
     4381 aattccgcat cagtaagcgc attggcccgc atgttctgat acaggttggt aaccagccag
     4441 tagtgcgcgt ttgatttcca cggataagac tccgcatccg gatacaggcc tcgcttccgg
     4501 caatactcgt aaaccatatc aaccagctcg ctgacgtttg gcagtccggc gataacggat
     4561 gcttcttccc ggcaccatgc aacaaactgc ccgggtgatg gcagaaatgg tcgattctgc
     4621 cgacgggcta cgcgcattcc tgcgttaacc tgttccattg tggtgatccc gttttcccgg
     4681 aaagccagaa cccactggcg gcggatttcg ttcagttcgt tctggtcacg gttagccagg
     4741 ctcgccggga aagttgccag taactggctg aacacaccgt tgatgatctg cgctacctgc
     4801 tgtacctgag gcttttcgtc gtactgttcc ggcatgttgt tggcgatccg acgcatctgc
     4861 tcacggtcaa agttaaccat ctgtgcggcg atgtttttca taaatccacc ccgtaaatcc
     4921 agtctgtgtt tgtcaggtcg agttttggtt tgctggctgt cacgactgcc tgttgcttgt
     4981 tacggttgat ttcgagctgg gtccacttgt cgcggagttt ggccgggctc agcacattac
     5041 cggaccagaa gttgtcctgg caggcccagc ggaacagcac acacatatcg cggtggttac
     5101 gtccgtcacg ttcacgcatc aggcggatat cgttagccca ccctgcaaaa ttcggttttc
     5161 tggctgatgg cgcgatagtc ttcaccatgt caaacatcca ctctgcggcg gtcaggtctt
     5221 ctgctgtccc ccatttgctg ccgctctgaa ttgcagcatc aggtttcacc acagaaaggt
     5281 cgttttctgg ctggtcagag gattcgccag aattctctga cgaataatct tttctatttt
     5341 cttttgtatt agtgtctttt gtgttcccct gttttgaggg atagcaatcc cccaatttga
     5401 gggatgtttt atccctcgtt ttaggggatt ttccctcgtt ttgagggata caccattctg
     5461 agatgttttt atttggtcca aacatgccgc cttgctgctt gataatattc attctgacga
     5521 gttctaactt ggcttcattg caccgtttga caggtaactt tgtaatctcg ctaagttgag
     5581 aatcggtgat tctgtccatt ggtttattcc acccataggt tttacgcaga atggcaagca
     5641 gcactttaaa ctgtcgcttg gtcagatctg cgcctgaata agcctcaagc agcatatttg
     5701 atagtctggc gtaaccatca tcgagatctg ccacattacg ctcctgtccg gcaaagttac
     5761 ctctgccgaa gttgagtatt tttgctgtat ttgtcataat gactcctgtt gatagttcca
     5821 gtaataacct cagaattcca tctggatttg ttcagaacgc tcggttgccg ccgggcgttt
     5881 tttattggtg agaatcgaag caacttgtcg tgccaatcga gccatgtcgt cgtcgacgac
     5941 accccattca agaacagcaa gcagcattga gaactttgga atccaatccc tcttccacct
     6001 gctgatctgc gacttatcaa ctcccacagc ttccgctgtc ttctcagttc caagcattgc
     6061 gattttgtta agcaacgcac tctcgattcg tagagcctcg ttgcgtttgt ttgcacgaac
     6121 catatgtaag tatttcctta gataacaatt gattgaatgt atgcaaataa atgcatacac
     6181 cataggtgtg gtttattttg atgccctttt tcagggctgg gatgtgtaag agcgggaatg
     6241 tcttaagcgg ctttaccgcg tttagttccg tactgtaacc aaaccggatc acagttaagc
     6301 gccatagcaa tctcaaacaa gaagcgcggt cgcttggtta ctccagcttc aatcagttga
     6361 attgattgct gtttaacacc ggctttggtt gccagttcgg tttgcgtcat ttttaacgca
     6421 attcgcctct tcttgaggcg ttcagaaaga gtttgcatat cgcctccatc aacaaacttt
     6481 cttgtatttt catacaatgt atcttgtttg tcaaatacag tttttcttgt gaagattggg
     6541 ggtaaataac agaggtggct tatgagtatt tcttccaggg taaaaagcaa aagaattcag
     6601 cttggactta accaggctga acttgctcaa aaggtgggga ctacccagca gtctatagag
     6661 cagctcgaaa acggtaaaac taagcgacca cgctttttac cagaacttgc gtcagctctt
     6721 ggcgtaagtg ttgactggct gctcaatggc acctctgatt cgaatgttag atttgttggg
     6781 cacgttgagc ccaaagggaa atatccattg attagcatgg ttagagctgg ttcgtggtgt
     6841 gaagcttgtg aaccctacga tatcaaggac attgatgaat ggtatgacag tgacgttaac
     6901 ttattaggca atggattctg gctgaaggtt gaaggtgatt ccatgacctc acctgtaggt
     6961 caaagcatcc ctgaaggtca tatggtgtta gtagatactg gacgcgagcc agtgaatgga
     7021 agccttgttg tagccaaact gactgacgcg aacgaagcaa cattcaagaa actggtcata
     7081 gatggcggtc agaagtacct gaaaggcctg aatccttcat ggcctatgac tcctatcaac
     7141 gggaactgca agattatcgg tgttgtcgtg gaagcgaggg taaaattcgt atgatcagga
     7201 ttgcggcgct actctcaata ctcttaacta ccagcgccaa ttctgaatgc tggattgtca
     7261 caaacctgca cgggtacggg gcaatgaatg gcgatcgtta cgagtttaca aaagacagca
     7321 cggaagattc cgttttcaac gtaacaataa atggcgataa atcatcagtt tatgaatcag
     7381 tttctggcgt ctatccagag atgaaataca ctgctttgtc atcgaacact atggtaggag
     7441 aataccagtc tggaggaggc ataaccgttg aaacttggtc aatcactaca gacaaaaaag
     7501 ctctttactc caaagtaatg aacatcccag gtatgcaaca acttacatca accaaatcct
     7561 ttgttggtga tgtagtcgga acctgcaatc agtaatcccc acctcaatct cgataacaaa
     7621 aaaacaaact attttccgtt taaaaacaat ggagtttgtt tttcacgccc ctttttacaa
     7681 tatttcttgt ttacaacata caatcttcct tgtaatttta agccatcagc aggacgcact
     7741 gaccaccatt gaaggtgagg ctcttaaaaa ttaagccctg aagaagggca gcattcaaag
     7801 cagaaggctt tggtgtgtgt gatacgaaac gaagcattgg ccggaagtgc gaatccggat
     7861 tagctgccaa tgtgccattg cggggtgttt tcgttcagga ctacgactcc cacacacaac
     7921 caaagctaac tgacaggaga atccagatgg atgcacaaac acgccgccgc gaacgtcgcg
     7981 cagagaaaca ggctcaatgg aaagcagcaa atcccctgtt ggttggggta agcgctaaac
     8041 cagttaaccg ccctattctc tcgctgaatc gcaaaccgaa atcacgagta gaaagcgcac
     8101 tgaatccgat agaccttacg gtgctggctg aataccacga acagattgaa agcaacctgc
     8161 aacgtattga gcgcaagaat catcgagttt ggtatagcaa gccaggtgag ttcggtataa
     8221 cttgtcaagg aagacaaaag gttaaagggg aatccattcc attggcatga ggtacgtaat
     8281 gaagaagatt gattacaagt ccataccaaa accaatagac tcagcatcag agcgaaaaaa
     8341 acacaaaaaa gaggctgaaa aattagcaaa ttatatcagt tttattagaa acaatgctca
     8401 cggcgatggc gacaagaagt tgctttcaga tgctcggacc caagcgttcg gtatacttcg
     8461 taagcagatg caatatcgtc ttcatcctgg ctacataatt gaaattcgcc ctactgaaag
     8521 acaattgttc ttattaaact ctgtctttga ctttgtaaac gtagttggag atcttatcga
     8581 caggtctgta gataaggatc ctgatacaaa tagttttctt ctaacaaata aagaatactt
     8641 atatggtaaa ttcggtataa acggatggca aaaatatgta cgcttcttac gtgcattcgt
     8701 tgatgcgtat aaaaattccg acatgattta tacatatttg cctggtggtg ataattgtac
     8761 gctttcggca gacaatagaa tattcatacc tgtacttggt cttgggctga ttaatgcagt
     8821 aaatgaaaaa gatgttataa ttgtta
//
LOCUS       DADSYM010000170         8714 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2415
            SAMN08039579-rid11723173.denovo.172, whole genome shotgun sequence.
ACCESSION   DADSYM010000170 DADSYM010000000
VERSION     DADSYM010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039579
            Sequence Read Archive: SRR6362533
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8714)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8714)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980162.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 03:39:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,484
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 4,347
            CDSs (with protein)               :: 4,347
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 43 of 85
            Pseudo Genes (incomplete)         :: 37 of 85
            Pseudo Genes (internal stop)      :: 19 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8714
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039579-rid11723173.denovo.172"
                     /strain="LGE2415"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8302 N 95.9628 W"
                     /collection_date="2015-08-24"
     gene            complement(<1..3838)
                     /gene="entF"
                     /locus_tag="J1Y83_003833"
     CDS             complement(<1..3838)
                     /gene="entF"
                     /locus_tag="J1Y83_003833"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin non-ribosomal peptide synthetase
                     EntF"
                     /protein_id="HBA7631937.1"
                     /translation="MSQHLPLVAAQPGIWMAEKLSDLPSAWSVAHYVELTGEVDAPLL
                     ARAVVAGLAQADTLRMRFTEDNGEVWQWVDDAQTFELPEIIDLRTNIDPHGTARALMQ
                     ADLQQDLRVDSGKPLVFHQLIQVADNRWYWYQRYHHLLVDGFSFPAITRQIANIYCAL
                     LRGEPTPASPFTPFADVVEEYQQYRESEAWQRDAAFWAEQRRQLPPPASLSPAPLPGR
                     SASADILRLKLEFTNGEFRQLATQLSGVQRTDLALALAALWLGRLCNRMDYAAGFIFM
                     RRLGSAALTATGPVLNVLPLGIHIAAQETLPQLATRLAAQLKKMRRHQRYDAEQIVRD
                     SGRAAGEEPLFGPVLNIKVFDYQLDIPGVQAQTHTLATGPVNDLELALFPDEHGDLSI
                     EILANKQRYDEPTLIQHAERLKMLIAQFAADPSLLCGDVDIMLPGEYAQLAQINATQV
                     EIPETTLSALVAEQAAKTPDAPALADARYQFSYREMREQVVALANLLRERGVKPGDSV
                     AVALPRSVFLTLALHAIVEAGAAWLPLDTGYPDDRLKMMLEDARPSLLITTDDQLPRF
                     SDISNLTSLCYNAPLTPQGSAPLQLSQPHHTAYIIFTSGSTGRPKGVMVGQQAIVNRL
                     LWMQNHYPLTGEDVVAQKTPCSFDVSVWEFFWPFIAGAKLVMAEPEAHRDPLAMQHFF
                     ADYGVTTTHFVPSMLAAFVASLTPQTARQSCATLKQVFCSGEALPADLCREWQQLTGA
                     PLHNLYGPTEAAVDVSWYPAFGEELAEVRGSSVPIGYPVWNTGLRILDAMMHPVPPGV
                     AGDLYLTGIQLAQGYLGRPDLTASRFIADPFAPGERMYRTGDVARWLDNGAVEYLGRS
                     DDQLKIRGQRIELGEIDRVMQALPDVEQAVTHACVINQAAATGGDARQLVGYLVSQSG
                     LPLDTSALQAQLRETLPPHMVPVVLLQLPQLPLSANGKLDRKALPLPELKAQTPGRVP
                     KAGSETIIAAAFSSLLGCDVQDADADFFALGGHSLLAMKLAAQLSRQFARQVTPGQVM
                     VASTVAKLATIIDGEEDSTQRMGFETILPLREGNGPTLFCFHPASGFAWQFSVLSRYL
                     DPQWSIIGIQSPRPHGPMQTAASLDEVCEAHLATLLEQQPHGPYYLLGYSLGGTLAQG
                     IAARLRARGEQVAFLGLLDTWPPETQNWQEKEANGLDPEVLAEINREREAFLAAQQGS
                     TSTELFTTIEGNYADAVRLLTTAHSVPFDGKATLFVAERTLQEGMTPEQAWAPWIAEL
                     DIYRQDCAHVDIISPVA"
     gene            complement(3835..4053)
                     /locus_tag="J1Y83_003834"
     CDS             complement(3835..4053)
                     /locus_tag="J1Y83_003834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbtH family protein"
                     /protein_id="HBA7631938.1"
                     /translation="MAFSNPFDDPQGAFYILRNAQGQFSLWPQQCALPAGWDVVCQPQ
                     SQASCQQWLEAHWRTLTPANFTQLQEAQ"
     gene            complement(4056..5258)
                     /gene="fes"
                     /locus_tag="J1Y83_003835"
     CDS             complement(4056..5258)
                     /gene="fes"
                     /locus_tag="J1Y83_003835"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterochelin esterase"
                     /protein_id="HBA7631939.1"
                     /translation="MTALKVGSESWWQSKHGPEWQRLNDEMFEVTFWWRDPQGSEEYS
                     TIKRVWVYITGVTDHHQNSQPRSMQRIAGTDVWQWTTQLNANWRGSYCFIPTERDDIF
                     SAPSPDRLELREGWRKLLPQAIADPLNPQSWKGGRGHAVSALEMPQASLQPGWDCPQA
                     PETPAKEIIWKSERLKNSRRVWIFTTGDATAEERPLAVLLDGEFWAQSMPVWPALTSL
                     THRRQLPPAVYVLIDAIDTTHRAHELPCNADFWLAVQQELLPQVKAIAPFSDRADRTV
                     VAGQSFGGLSALYAGLHWPERFGCVLSQSGSYWWPHRGGHQEGMLLEQLNTGEVSAEG
                     LRIVLEAGVREPMIMQANQALYAQLHPLKESIFWRQVDGGHDALCWRGGLMQGLIDLW
                     QPLFHDRS"
     gene            5501..7741
                     /gene="fepA"
                     /locus_tag="J1Y83_003836"
     CDS             5501..7741
                     /gene="fepA"
                     /locus_tag="J1Y83_003836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore enterobactin receptor FepA"
                     /protein_id="HBA7631940.1"
                     /translation="MNKKIHSLALLVNLGIYGVAQAQEPTDTPVSHDDTIVVTAAEQN
                     LQAPGVSTITADEIRKNPVARDVSEIIRTMPGVNLTGNSTSGQRGNNRQIDIRGMGPE
                     NTLILIDGKPVSSRNSVRQGWRGERDTRGDTSWVPPEMIERIEVLRGPAAARYGNGAA
                     GGVVNIITKKGSGEWHGSWDAYFNAPEHKEEGATKRTNFSLTGPLGDEFSFRLYGNLD
                     KTQADAWDINQGHQSARAGTYATTLPAGREGVINKDINGVVRWDFAPLQSLELEAGYS
                     RQGNLYAGDTQNTNSDAYTRSKYGDETNRLYRQNYSLTWNGGWDNGVTTSNWVQYEHT
                     RNSRIPEGLAGGTEGKFNEKAAQDFVDIDLDDVMLHSEVNLPIDFLVNQTLTLGTEWN
                     QQRMKDLSSNTQALTGTNTGGAIDGVSATDRSPYSKAEIFSLFAENNMELTDSTIVTP
                     GLRFDHHSIVGNNWSPALNISQGLGDDFTLKMGIARAYKAPSLYQTNPNYILYSKGQG
                     CYASAGGCYLQGNDDLKAETSINKEIGLEFKRGGWLAGVTWFRNDYRNKIEAGYVAVG
                     QNAVGTDLYQWDNVPKAVVEGLEGSLNVPVSETVMWTNNITYMLKSENKTTGDRLSII
                     PEYTLNSTLSWQAREDLSMQTTFTWYGKQQPKKYNYKGQPAVGPETKEISPYSIVGLS
                     ATWDVTKNVSLTGGVDNLFDKRLWRAGNAQTTGDLAGANYIAGAGAYTYNEPGRTWYM
                     SINTHF"
     gene            7916..8536
                     /gene="entD"
                     /locus_tag="J1Y83_003837"
     CDS             7916..8536
                     /gene="entD"
                     /locus_tag="J1Y83_003837"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415115.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin synthase subunit EntD"
                     /protein_id="HBA7631941.1"
                     /translation="MKTTHTSLPFAGHTLHFVEFDPASFREQDLLWLPHYAQLQHAGR
                     KRKTEHLAGRIAAIYALREYGYKCVPAIGELRQPVWPAGVYGSISHCGTTALAVVSRQ
                     PIGIDIEEIFSAQTARELTDNIITPAEHERLAECGLTFSLALTLAFSAKESAFKASEI
                     QTDADFLDYQIISRNKQQVIIHRENEMFAVHWQIKEKIVITLCQHD"
BASE COUNT         2087 a         2404 c         2339 g         1884 t
ORIGIN      
        1 acgccaccgg agagataata tccacatgcg cacaatcctg acgatagata tccagctcag
       61 caatccacgg tgcccaggcc tgttcaggag tcataccttc ctgaagcgtg cgttcggcaa
      121 caaatagcgt cgctttacca tcaaacggta cgctatgagc tgtcgtcagc aggcgcacag
      181 catcagcgta gttgccttca atggtggtaa acaactccgt tgaagtactt ccctgctgtg
      241 ccgccaggaa ggcttcgcgc tcgcggttaa tctccgccag cacttccggg tccagaccat
      301 tggcttcttt ttcctgccag ttttgcgttt ctggcggcca ggtatccagc aagccaagaa
      361 atgccacctg ttcgccacgg gcacgcagtc gcgccgcaat gccctgcgcc agcgtaccgc
      421 caagtgaata acccagcagg taatatgggc cgtgcggttg ttgttcaagt aaggttgcca
      481 gatgcgcttc gcagacttca tccaggcttg ccgccgtctg catggggcca tgagggcgcg
      541 gcgactgaat gccgataatc gaccattgtg gatcgagata acgcgagagc acgctgaact
      601 gccaggcaaa accggatgcc ggatggaaac aaaacagcgt cgggccatta ccttcacgca
      661 acggcagaat ggtttcgaat cccatgcgct gggtgctgtc ttcttcacca tcaataatcg
      721 ttgccagttt ggcgacagtt gacgcgacca tcacctgccc cggcgtcacc tgacgagcaa
      781 actgtcgact taactgcgct gccagtttca ttgccagtag cgaatgaccg ccaagcgcaa
      841 agaaatcagc atcggcatcc tgcacgtcac aacccagcaa cgacgagaat gccgcggcga
      901 taatcgtttc actgcccgct ttcggcacac gccccggcgt ttgtgccttc agttcaggca
      961 acggtaaggc tttgcgatcc agcttgccgt tggcgctaag tggtaactgt ggaagttgca
     1021 gcagaaccac cggtaccata tgtggtggca atgtttctcg aagctgcgcc tgtaatgcgc
     1081 tggtatccaa cggcagacct gattgcgaca ccagatagcc caccaactga cgcgcatcac
     1141 caccagtggc tgccgcctgg ttaatcacac aggcgtgggt aacggcttgt tcgacatccg
     1201 gcagcgcctg catcacgcga tcaatttcgc ccagttcgat acgctgcccg cgaattttta
     1261 gctgatcgtc actgcgcccg aggtactcca ccgcgccgtt atccagccag cgggccacat
     1321 cgccggtacg gtacatccgt tcacctgggg caaaaggatc ggcaataaag cggctggcgg
     1381 tcagatcggg tcgtccaaga tacccctgcg ccagttgaat accggtgaga tagagatctc
     1441 ccgccacacc cggcggcacc ggatgcatca tcgcatcgag aatgcgcagg cccgtattcc
     1501 acaccggata accgatcggc acactgctgc cgcgcacctc tgccagttcc tcgccaaaag
     1561 ccggatacca actgacatct accgccgctt ccgtcgggcc atatagatta tgcaacggcg
     1621 cgccggttaa ctgttgccat tcgcggcata aatcagccgg taaggcttca ccactacaga
     1681 aaacctgttt caacgtcgcg caactctggc gagcggtttg cggcgtcagc gatgcaacaa
     1741 atgccgccag catcgacggc acaaagtgcg tggtcgttac gccataatca gcaaaaaaat
     1801 gctgcatagc gagcgggtcg cggtgcgctt ccggttcagc catcacaagt ttcgccccgg
     1861 caataaacgg ccagaaaaac tcccacaccg agacatcaaa actgcacggc gttttttggg
     1921 caacaacatc ttcgcctgta agcgggtaat ggttttgcat ccatagcagg cggttaacga
     1981 tagcctgctg accgaccatt acccctttcg gtctgccggt ggagccagag gtaaagatga
     2041 tataagccgt gtggtgcggt tgtgaaagct gcagcggcgc actgccctgc ggtgtcagcg
     2101 gggcgttata gcaaaggctg gttaaattgg agatatcgct aaagcgcggc agttgatcgt
     2161 cagtggtgat taacagcgac ggacgcgcat cttccagcat cattttcagg cgatcgtccg
     2221 gatagccggt atccaacggt agccaggccg caccagcttc aactatcgca tgtagcgcca
     2281 gggtcaaaaa gaccgagcgc ggtagagcca ccgccacgct gtcgcccggt ttaacgccgc
     2341 gctcacgcag cagattcgcc agcgccacca cctgctcgcg catttcccga tagctgaact
     2401 ggtaacgcgc atctgccagc gccggagcat ccggtgtttt tgccgcttgt tctgccacca
     2461 gcgcgctcag cgtggtttct ggaatctcaa cctgagtggc gttgatctgc gccagctgcg
     2521 catactcacc tggcagcata atatcgacat cgccgcacaa cagcgacgga tccgcggcga
     2581 actgcgcaat cagcattttc aggcgttcag cgtgttgaat taacgttggc tcatcgtaac
     2641 gctgtttatt ggcgaggatt tcaatactca aatcaccgtg ttcatccggg aacagggcca
     2701 gttcaaggtc attaaccgga ccggttgcca gggtatgggt ttgcgcctga acaccaggaa
     2761 tatccagttg gtaatcaaat accttgatat tgagtaccgg accgaacagc ggttcctctc
     2821 ctgccgctcg cccgctgtca cggacaattt gttcggcatc gtaacgttga tggcgacgca
     2881 tttttttcag ttgagctgcc agtcgggttg ccagttgcgg cagcgtttct tgcgccgcaa
     2941 tgtgaatacc caatggcaaa acgttgagca cgggtccggt agccgtcagc gccgccgagc
     3001 ccagtcgacg cataaagata aatccggcag cgtagtccat gcggttgcac aatcgcccca
     3061 gccacaaggc tgccaacgca agtgctaaat cggtacgctg cacacctgaa agttgcgtag
     3121 ccagctggcg gaattccccg ttggtaaatt ccagtttcag gcgcaaaata tctgccgaag
     3181 cgctgcgccc cggtaaaggt gccggagaaa gtgacgcggg cggtggtagt tgacgacgct
     3241 gttccgccca gaatgccgca tcacgctgcc aggcttcact ttcgcggtat tgctggtact
     3301 cttccacgac atcagcgaaa ggcgtaaacg gcgaagcagg cgttggttcg ccacgcagca
     3361 atgcgcagta aatgttggcg atctggcgtg taatggccgg gaaactgaag ccatcgacca
     3421 gcaaatggtg ataacgctga taccagtacc agcggttatc cgccacctgt atcagttgat
     3481 gaaagaccag tggtttacca ctatcgacgc gcagatcttg ttgcaaatcc gcctgcatta
     3541 atgcccgcgc agtaccgtgc ggatcaatgt tggttcgcag gtcgataatt tctggcagtt
     3601 cgaacgtctg cgcatcatcg acccactgcc agacttcgcc gttatcttcc gtaaaacgca
     3661 tccgcaacgt atctgcttgc gctagtccgg caaccaccgc gcgggccagt agtggcgcat
     3721 caacctctcc ggttaactca acgtaatgcg ccacgctcca ggcggagggt aaatccgaca
     3781 gtttttctgc catccagatg ccgggctgtg cggcgaccaa aggtaaatgc tggctcattg
     3841 tgcctcctgc aactgggtga aattcgccgg tgtcagagta cgccagtggg cttccagcca
     3901 ctgctggcag gacgcctgtg actgcggctg acacacaacg tcccagcctg ccggtaaggc
     3961 gcattgttgc ggccacagac tgaattgccc ctgcgcattg cgcaaaatgt aaaacgctcc
     4021 ctgtggatca tcgaagggat tactgaatgc catattcaac tcctgtcatg gaaaagtggt
     4081 tgccagaggt cgattagccc ctgcatcaag ccaccgcgcc aacaaagcgc atcatgtccg
     4141 ccgtcaacct gacgccagaa aatggattct tttagggggt gtaattgcgc atacagcgcc
     4201 tgattggcct gcatgatcat cggctcgcga acacccgctt ccagcacaat gcgcagacct
     4261 tcggcgctaa cttcaccagt atttagctgt tcaagtaaca tgccctcttg gtgcccgccc
     4321 cgatgcggcc accagtacga acctgactgg cttaatacac agccaaagcg ttcaggccag
     4381 tgcagtccgg catacagcgc ggaaagccca ccaaaactct gtccggcgac cacggtgcga
     4441 tcggcgcgat cgctaaaagg ggcaatagct ttcacctggg gtaataactc ttgctgtact
     4501 gccagccaga aatccgcatt acacggcagt tcgtgggcgc ggtgcgtggt gtcgatagcg
     4561 tcgatcaaca catacacggc gggcggaagt tgccgacgat gggtcagcga agtcagcgca
     4621 ggccagacgg gcatactctg cgcccaaaat tcgccatcaa gcaaaactgc cagcgggcgt
     4681 tcttctgctg ttgcatcgcc ggtggtaaaa atccatacac ggcgtgaatt tttcaaccgt
     4741 tcacttttcc agataatttc tttggcaggc gtttctggcg cttgcggaca atcccatccc
     4801 ggttgcagag acgcttgcgg catttcgagt gcagaaacag cgtgccctcg cccgcctttc
     4861 cagctttgtg gattcagcgg atcggctatc gcctggggta atagttttcg ccagccttcg
     4921 cgtaattcga ggcgatcggg ggatggtgca gaaaaaatgt catcgcgttc agtaggaata
     4981 aagcagtagc tgccacgcca gttggcattg agttgtgtcg tccactgcca aacatcggtt
     5041 cctgcaattc gctgcatcga ccggggctgg ctgttctgat ggtgatcggt cacgccagtg
     5101 atatagaccc atacgcgctt tatcgtcgag tattcttcag aaccctgggg atcacgccac
     5161 cagaaagtga cctcaaacat ttcgtcattc agacgttgcc attccgggcc atgtttcgac
     5221 tgccaccagc tctcacttcc tacttttaac gccgtcacac cataacccca tgtttactgt
     5281 gcaatttttc attgattgca gaaatatatt gataatatta ttgataacta tttgcatttg
     5341 caatagcgta atggcgcgcc gtgggaagcg cggacattaa ttaatcagct gcactgcgtg
     5401 tctttcagga tcaaaggttt tcgcggtagc gggatgcgtc gtgttgatga cgaccatgcc
     5461 cgacagttgc aattcgtggc aaaaatgcag gaataaaaca atgaacaaga agattcattc
     5521 cctggccttg ttggtcaatc tggggattta tggggtagca caggcacaag agccgaccga
     5581 tactcctgtt tcacatgacg atactattgt tgttaccgcc gccgagcaga acttgcaggc
     5641 gcccggcgtt tcgaccatca ccgcagatga aatccgcaaa aacccggttg cccgcgatgt
     5701 atcggagatt atccgtacca tgcctggcgt taacctgacc ggtaattcca ccagtggtca
     5761 gcgtggtaat aaccgccaga ttgatattcg cggcatgggt ccggaaaaca cgctgatttt
     5821 gattgacggc aagccggtaa gcagccgtaa ctctgtgcgt cagggctggc gtggcgagcg
     5881 cgatacccgt ggtgatacct cctgggtgcc gcctgaaatg attgaacgta ttgaagttct
     5941 gcgtggtccg gcagctgcgc gttatggcaa cggcgcggcg ggcggcgtgg ttaacatcat
     6001 taccaaaaaa ggcagtggtg agtggcacgg ctcctgggat gcttatttca atgcgccaga
     6061 acataaagag gaaggtgcca ccaaacgtac caacttcagc ctgaccggtc cgctgggcga
     6121 cgaattcagc ttccgcttgt atggcaacct cgacaaaacc caggctgacg cgtgggatat
     6181 caaccagggc catcagtccg cgcgtgccgg aacgtatgcc acgacgttac cagccgggcg
     6241 cgaaggggtg atcaacaaag atattaatgg cgtggtgcgc tgggacttcg cgcctctgca
     6301 gtcactcgaa ctggaagcgg gctacagccg ccagggtaac ctgtatgcgg gtgatacgca
     6361 gaacaccaac tctgacgctt acactcgctc gaaatatggc gatgaaacca accgcctgta
     6421 tcgccagaac tactcgctga cctggaacgg tggctgggat aacggcgtga ccaccagcaa
     6481 ctgggtgcag tacgaacaca cccgtaactc gcgtattccg gaaggtctgg cgggcggtac
     6541 cgaagggaaa tttaacgaaa aagcggcaca ggatttcgtc gatatcgatc ttgatgacgt
     6601 gatgctgcac agcgaagtta acctgccgat tgatttcctc gttaaccaaa cgctgacgct
     6661 gggtacggag tggaatcagc aacggatgaa ggacttaagt tccaacaccc aggcgctgac
     6721 cggaacgaat accggcggtg ctattgatgg cgtgagtgcc accgaccgta gcccatattc
     6781 aaaagcagaa attttctcgc tgtttgccga aaacaatatg gagctgactg acagtaccat
     6841 cgtaacgccg gggctgcgtt tcgatcatca cagtattgtc ggcaataact ggagcccggc
     6901 gctgaacata tcgcaaggtt taggcgatga cttcacgctg aaaatgggca tcgcccgcgc
     6961 ctataaagcg ccgagcctgt accagactaa cccaaactac attctctaca gtaaaggtca
     7021 gggctgctat gccagcgcgg gcggctgcta tctgcaaggt aatgatgacc tgaaagcaga
     7081 aaccagcatc aacaaggaga ttggtctgga gttcaaacgc ggcggctggc tggcgggcgt
     7141 gacctggttc cgtaacgatt atcgcaataa gattgaagcg ggctatgtgg ctgtagggca
     7201 aaacgcagtc ggcaccgatc tctatcagtg ggataacgta ccgaaagcgg tggttgaagg
     7261 tctggaagga tcgttaaacg taccggttag cgaaacggtg atgtggacca ataacatcac
     7321 ttatatgctg aagagtgaaa acaaaaccac gggcgaccgt ttgtcgatca tcccggaata
     7381 tacgttgaac tcaacgctga gctggcaggc acgggaagat ttgtcgatgc aaacgacctt
     7441 cacctggtac ggcaagcagc agccgaagaa gtacaactac aaaggtcagc cagcggttgg
     7501 accggaaacc aaagaaatca gtccgtacag cattgttggc ctgagcgcga cctgggatgt
     7561 gaccaagaat gtcagtctga ccggcggcgt ggacaacctg ttcgacaaac gtttgtggcg
     7621 tgcgggtaat gcccagacca cgggcgatct ggcaggagcc aactatatcg caggtgccgg
     7681 ggcgtatacc tataacgagc cgggacgtac gtggtatatg agcattaata ctcacttctg
     7741 atgctaacgt cagattgttg acaaagtgcg cgtagttcat gccggatgcg gcgtgaacgc
     7801 cttatccggc ctacaaaaat cttgccaatt caatatattg caggaccacg taggcctgat
     7861 aagcgtagcg catcaggcag ttttgcgttt gtcatcattc tctaatatgg ttgacatgaa
     7921 aactacgcat acctccctcc cctttgcagg acatacgctg cattttgttg agtttgatcc
     7981 ggcgagtttt cgcgagcagg atttactctg gctgccgcac tacgcacaac ttcaacacgc
     8041 tggacgtaaa cgtaaaacag agcatttagc cgggcggatc gctgcaattt atgcgctgcg
     8101 ggaatatggc tataaatgtg tgcctgcaat cggcgagcta cgccaacctg tgtggcctgc
     8161 gggggtatac ggcagcatta gtcactgtgg gactacggca ttagccgtgg tttcccgtca
     8221 accgattggc attgatattg aagagatttt ctctgcacaa accgcaagag aattgacaga
     8281 caacattatt acaccagcag aacacgagcg actcgcagaa tgcggtttaa ccttttctct
     8341 ggcgctgaca ctggcatttt ccgccaaaga gagcgcattt aaggcaagtg aaatccaaac
     8401 tgatgcagat tttctggact atcagataat tagccggaat aaacaacagg tcataattca
     8461 tcgtgagaat gagatgtttg ctgtgcactg gcagataaaa gaaaaaatag tcataacgct
     8521 gtgccaacac gattaattga caacatctgg tacgattcgc ccgcagccat ctctgaccgc
     8581 gggcgaaagt gtaaagcagg tgccttacca ccctgacctg acaaccggat atgcggggat
     8641 tgctccccgc acaacggcta cttcttcgat tcgtaagcga gaacagcctt aaactcaata
     8701 ttacgttcct ttac
//
LOCUS       DADSYM010000171         8578 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2415
            SAMN08039579-rid11723173.denovo.173, whole genome shotgun sequence.
ACCESSION   DADSYM010000171 DADSYM010000000
VERSION     DADSYM010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039579
            Sequence Read Archive: SRR6362533
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8578)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8578)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980162.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 03:39:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,484
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 4,347
            CDSs (with protein)               :: 4,347
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 43 of 85
            Pseudo Genes (incomplete)         :: 37 of 85
            Pseudo Genes (internal stop)      :: 19 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8578
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039579-rid11723173.denovo.173"
                     /strain="LGE2415"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8302 N 95.9628 W"
                     /collection_date="2015-08-24"
     gene            111..653
                     /gene="ecpR"
                     /locus_tag="J1Y83_003838"
                     /gene_synonym="matA"
     CDS             111..653
                     /gene="ecpR"
                     /locus_tag="J1Y83_003838"
                     /gene_synonym="matA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECP biosynthesis operon DNA-binding
                     transcriptional regulator EcpR"
                     /protein_id="HBA7631942.1"
                     /translation="MECQNRSDKYIWSPHDAYFYKGLSELIVDIDRLIYLSLEKIRKD
                     FVFINLNTDSLTEFINRDNEWLSAVKGKQVVLIAARKSEALANYWYYNSNIRGVVYAG
                     LSRDIRKELAYVINGRFLRKDIKKDKITDREMEIIRMTAQGMLPKSIARIENCSVKTV
                     YTHRRNAEAKLYSKLYKLVQ"
     gene            727..1314
                     /gene="ecpA"
                     /locus_tag="J1Y83_003839"
                     /gene_synonym="matB"
     CDS             727..1314
                     /gene="ecpA"
                     /locus_tag="J1Y83_003839"
                     /gene_synonym="matB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="common pilus major fimbrillin subunit EcpA"
                     /protein_id="HBA7631943.1"
                     /translation="MKKKVLAIALVTVFTGTGVAQAADVTAQAVATWSATAKKDTTSK
                     LVVTPLGSLAFQYAEGIKGFNSQKGLFDVAIEGDSTATAFKLTSRLITNTLTQLDTSG
                     STLNVGVDYNGAAVEKTGDTVMIDTANGVLGGNLSPLANGYNASNRTTAQDGFTFSII
                     SGTTNGTTAVTDYSTLPEGIWSGDVSVQFDATWTS"
     gene            1371..2039
                     /locus_tag="J1Y83_003840"
     CDS             1371..2039
                     /locus_tag="J1Y83_003840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone EcpB"
                     /protein_id="HBA7631944.1"
                     /translation="MKKHLLPLALLFSGISPAQALDVGDISSFMNSDSSTLSKTIQNS
                     TDSGRLINIRLERLSSPLDDGQVIAMDKPDELLLTPASLLLPAQASEVIRFFYKGPAD
                     EKERYYRIVWFDQALSDAQRDNANRSAVATASARIGTILVVAPRQANYHFQYANGSLT
                     NTGNATLRILAYGPCLKAANGKECKENYYLMPGKSRRFTRVDTADNKGRVALWQGDKF
                     IPVK"
     gene            2065..4590
                     /locus_tag="J1Y83_003841"
     CDS             2065..4590
                     /locus_tag="J1Y83_003841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA7631945.1"
                     /translation="MPLRRFSPGLKAQFAFGMVFLFVQPDASAADISAQQIGGVIIPQ
                     AFSQALQDGMSVPLYIHLAGSQGRQDDQRIGSAFIWLDDGQLRIRKIQLEESEDNASV
                     SEQTRQQLMTLANAPFNEALTIPLTDNAQLDLSLRQLLLQLVVKREALGTVLRSRSED
                     IGQSSVNTLSSNLSYNFGVYNNQLRNGGSNTSSYLSLNNVTALREHHVVLDGSLYGIG
                     SGQQDSELYKAMYERDFAGHRFAGGMLDTWNLQSLGPMTAISAGKIYGLSWGNQASST
                     IFDSSQSATPVIAFLPAAGEVHLTRDGRLLSVQNFTMGNHEVDTRGLPYGIYDVEVEV
                     IVNGRVISKRTQRVNKLFSRGRGVGAPLAWQIWGSSFHMDRWSENGKKTRPAKESWLA
                     GASTSGSLSTFSWAATGYGYDNQAVGETRLTLPLGGAINVNLQNMLASDSSWSNIASI
                     SATLPGGFSSLWVNQEKTRIGNQLRRSDADNRAIGGTLNLNSLWSKLGTFSISYNDDR
                     RYNSHYYTADYYQSVYSGTFGSLGLRAGIQRYNNGDSSANTGKYIALDLSLPLGNWFS
                     AGMTHQNGYTMANLSARKQFDEGTIRTVGANLSRAISGDTGDDKTLSGGAYAQFDARY
                     ASGTLNVNSAADGYINTNLTANGSVGWQGKNIAASGRTDGNAGVIFDTGLENDGQISA
                     KINGRIFPLNGKRNYLPLSPYGRYEVELQNSKNSLDSYDIVSGRKSHLTLYPGNVAVI
                     EPEVKQMVTVSGRIRAEDGTLLANARINNHIGRTRTDENGEFVMDVDKKYPTIDFRYS
                     GNKTCEVALELNQARGAVWVGDVVCSGLSSWAAVTQTGEENES"
     gene            4580..6223
                     /gene="ecpD"
                     /locus_tag="J1Y83_003842"
     CDS             4580..6223
                     /gene="ecpD"
                     /locus_tag="J1Y83_003842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial adhesin EcpD"
                     /protein_id="HBA7631946.1"
                     /translation="MRVNLLIAMIIFALIWPATALRAAVSKTTWADAPAREFVFVENN
                     SDDNFFVTPGGALDPRLTGANRWTGLKYNGSGTIYQQSLGYIDNGYNTGLYTNWKFDM
                     WLENSPVSSPLTGLRCINWYAGCNMTTSLILPQTTDASGFYGATVTSGGAKWMHGMLS
                     DAFYQYLQQMPVGSSFTMTINACQTSVNYDASSGARCKDQASGNWYVRNVTHTKAANL
                     RLINTHSLAEVFINSDGVPTLGEGNADCRTQTIGSRSGLSCKMVNYTLQTNGLSNTSI
                     HIFPAIANSSLASAVGAYDMQFSLNGSSWKPVSNTAYYYTFNEMKSADSIYVFFSSNF
                     FKQMVNLGISDINTKDLFNFRFQNTTSPESGWYEFSTSNTLIIKPRDFSISIISDEYT
                     QTPSREGYVGSGESALDFGYIVTTSGKTAADEVLIKVTGPAQVIGGRSYCVFSSDDGK
                     AKVPFPATLSFITRNGATKTYDAGCDDSWRDMTDALWLTTPWTDISGEVGQMDKTTVK
                     FSIPMDNAISLRTVDDNGWFGEVSASGEIHVQATWRNIN"
     gene            6192..6902
                     /locus_tag="J1Y83_003843"
     CDS             6192..6902
                     /locus_tag="J1Y83_003843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308346.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone EcpE"
                     /protein_id="HBA7631947.1"
                     /translation="MFRRRGVTLTKALLTVVCMLAAPLTQAISVGNLTFSLPSETDFV
                     SKRVVNNNKSARIYRIAISAIDSPGSSELRTRPVDGELLFAPRQLALQAGESEYFKFY
                     YHGPRDNRERYYRVSFREVPTRNLTKRSPTGGEVSTEPVVVMDTILVVRPRQVQFKWS
                     FDQVTGTVSNTGNTWFKLLIKPGCDSTEEEGDAWYLRPGDVVHQPELRQPGNHYLVYN
                     DKFIKISDSCPAKPPSAD"
     gene            7215..7544
                     /locus_tag="J1Y83_003844"
     CDS             7215..7544
                     /locus_tag="J1Y83_003844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HBA7631948.1"
                     /translation="MSNLNPCMTCGACCAFFRVSFYWAEADDAGGTIPARLTEQISPF
                     HRCMSGTNQKNPRCIALAGTPGKNACCTIYENRSSTCREFAMSGENGEVNEACNRARA
                     KYGLTPL"
     gene            complement(7556..7719)
                     /locus_tag="J1Y83_003845"
                     /pseudo
     CDS             complement(7556..7719)
                     /locus_tag="J1Y83_003845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001423098.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
     gene            complement(7793..8407)
                     /locus_tag="J1Y83_003846"
     CDS             complement(7793..8407)
                     /locus_tag="J1Y83_003846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YagU family protein"
                     /protein_id="HBA7631949.1"
                     /translation="MNIFEQTPPNRRRYGLAAFIGLIAGVVSAFVKWGAEVPLPPRSP
                     VDMFNAACGPESLIRAAGQIDCSRNFLNPPYIFLRDWLGLTDPNSAVYTFAGHVFNWV
                     GVTHIIFSIVFAVGYCVVAEVFPKIKLWQGLLAGALAQLFVHMISFPLMGLTPPLFDL
                     PWYENVSEIFGHLVWFWSIEIIRRDLRNRITHEPDPEIPLGSNR"
BASE COUNT         2291 a         2174 c         2159 g         1954 t
ORIGIN      
        1 atttacttcc gatttactaa agaaactgaa tgtacctgta aaaattacag gtttggaaag
       61 tagtgacatg gcaaaatgat tacagcaggg actatgaggt taaaaaccat atggaatgtc
      121 aaaaccgttc tgataaatac atctggtctc cccatgacgc ctacttctat aaaggactat
      181 ctgaactgat tgtggatatc gacagattaa tttatctatc gctggagaaa atcagaaaag
      241 atttcgtgtt tatcaatctc aatacggatt ctttaactga gtttataaac cgtgataatg
      301 agtggttatc cgcggtaaag gggaaacagg tcgtattgat tgcggccaga aagtcagaag
      361 ccttagcaaa ttattggtat tacaacagca atattagggg cgtggtatac gctggactga
      421 gtcgtgatat tagaaaagaa ctggcctatg tgattaatgg caggttcctg agaaaagata
      481 ttaagaaaga taaaatcact gaccgggaaa tggaaattat ccgcatgacg gctcagggaa
      541 tgctgcctaa atcgattgcc agaattgaaa attgtagtgt gaagacagtg tatacccatc
      601 ggcggaatgc agaggccaag ctgtactcaa aattatataa gttggttcag taaactccag
      661 gcaagttagt tttaaaaatg actcaccggg acttcatgtc ctcaattcaa ctcgggaaga
      721 aaagcaatga aaaaaaaggt tctggcaata gctctggtaa cggtgtttac cggtacaggt
      781 gtagcgcagg ctgctgacgt aacagctcag gctgtagcga cctggtcagc aacagccaaa
      841 aaagacacca ccagtaagct ggttgtgacg ccactcggta gcctggcgtt ccagtatgcc
      901 gaaggcatta aaggttttaa ctcacagaaa ggtctatttg acgtggctat cgagggtgac
      961 tcaacggcta ccgcctttaa actgacctca cgtcttatca ccaacacctt aacccagttg
     1021 gatacctcag gatccacact gaatgtgggc gtggattata acggcgcggc agtcgaaaaa
     1081 actggcgata ccgtgatgat cgataccgcc aacggcgtac tgggcggcaa ccttagccca
     1141 ctggctaacg gttacaatgc cagcaatcgt accaccgcac aggatggttt cactttctcc
     1201 atcatcagcg gcaccaccaa tggtaccacc gcagtaaccg attacagcac tctaccggaa
     1261 ggcatctgga gcggcgacgt tagcgtacag ttcgacgcta cctggaccag ttaatctctc
     1321 tgatgtacca gcaggggtac ccccctgctt tattcctgga cggaattgtt atgaaaaagc
     1381 accttctgcc tctcgctctg ctgttttccg gaatatcacc ggcccaggcg ctggatgtcg
     1441 gcgatatatc atcgtttatg aacagtgaca gcagcacgct aagcaaaacg atccaaaaca
     1501 gtaccgacag tggccgcctc atcaatatcc gtctcgaacg gctctcttca ccgcttgacg
     1561 acgggcaggt tatcgcaatg gacaagccgg atgagttgct actcactccc gccagcttgc
     1621 tgctacccgc ccaagccagc gaagtgatcc gcttcttcta taagggacca gcagatgaaa
     1681 aagagcgcta ctaccgcatt gtctggtttg atcaggccct cagtgatgcg cagcgcgata
     1741 atgccaaccg tagcgctgtg gccactgctt ccgcccgcat cggcaccatt ctggtcgtcg
     1801 caccccgcca ggcaaactac cactttcagt acgccaacgg ctccctgaca aatacaggaa
     1861 atgcgacgct gcggatcctc gcctacggcc cctgcctgaa agccgccaat ggtaaggagt
     1921 gtaaagagaa ttactacctg atgccgggca agtcgcgtcg ttttacccgc gtggacacgg
     1981 cggataacaa aggacgggtt gcactttggc agggtgataa gttcattccc gtgaaataga
     2041 tagctgtgca gatggataac gacaatgcct ttacgacggt tctccccagg actgaaagcc
     2101 cagtttgcct tcggcatggt ctttttgttc gttcagcccg atgccagcgc tgctgacata
     2161 agtgcgcagc aaataggtgg ggtgattatt ccgcaggcct tcagtcaggc gctccaggac
     2221 ggcatgagcg tcccgcttta tattcatctc gccggtagcc agggtcgcca ggacgatcag
     2281 cgaatcggca gcgcttttat ctggttggat gatggacagt tacgcatccg gaaaatacag
     2341 ctggaagaga gtgaagataa cgccagtgtc agcgaacaaa ctcgacagca actgatgact
     2401 ctggccaacg ccccgttcaa tgaggccctt accatccccc tgactgacaa cgcgcagttg
     2461 gatctcagct tgcgccaact gctgctgcag ctggtggtca agcgcgaagc gctgggcacc
     2521 gtactacgct cacgtagcga agacatcggg cagtccagtg ttaacaccct cagcagtaat
     2581 ctgagctata acttcggcgt ctataacaac cagttgcgta acggcgggag caacacctcc
     2641 agctatctgt cgctgaataa cgttactgcg ctgcgcgaac atcacgtggt gctcgacggc
     2701 tcactgtacg ggattggtag cggtcaacag gacagtgaat tatataaagc gatgtatgaa
     2761 cgcgattttg ctggtcaccg atttgccggt ggaatgctcg acacctggaa cttgcagtcc
     2821 ttagggccga tgaccgccat ttcagcaggg aagatttacg gcctttcctg gggaaaccag
     2881 gccagctcca ccatattcga cagcagccag tcggccacgc cagtgatcgc ctttttaccg
     2941 gcggcgggcg aagtacatct cacccgtgat gggcggctac taagcgttca gaacttcacc
     3001 atgggcaatc atgaagtgga tacccggggt ctaccgtacg gtatttacga tgtggaagtt
     3061 gaggtaatcg ttaacggtcg cgtgatcagc aaacgcaccc agcgggtcaa taagctgttt
     3121 agccgggggc gtggcgtcgg tgcaccgctg gcgtggcaga tatggggcag tagctttcat
     3181 atggatcgct ggtcggaaaa cgggaaaaag acgcgaccag ccaaagagag ttggctggca
     3241 ggtgcctcga cctccggctc actgagtacg tttagctggg cggcaacggg atatggatac
     3301 gataatcagg cggtgggtga aacccgtctg acgctgccgc ttgggggggc gatcaacgtt
     3361 aacctgcaaa acatgctggc cagtgacagc tcatggagca acatcgccag catcagcgcc
     3421 actctacctg gaggcttcag ttcgctgtgg gttaaccagg aaaaaacccg cattggcaat
     3481 caattgcgac gtagcgatgc cgacaaccgt gcaatcggcg gcacactcaa cctgaactca
     3541 ctgtggtcga agctgggtac gttcagcatc agctacaatg atgaccgccg ctacaacagc
     3601 cattattaca cggcagatta ctatcaaagt gtctacagcg gtacctttgg ttcgcttggc
     3661 ctgcgggccg gtattcagcg ctataacaac ggcgacagca gcgccaatac agggaaatat
     3721 atcgctctcg atctctcgct accactgggc aactggttta gcgcagggat gacccatcaa
     3781 aacggctaca ccatggcaaa cctgtcagca cgcaaacagt ttgatgaagg aaccattcgc
     3841 actgttggtg ccaatctgtc acgagccatc tccggcgata ccggtgatga caaaaccctc
     3901 agcggtgggg cgtatgcaca gttcgacgct cgttacgcca gcggaacgct gaacgtcaat
     3961 agcgcggcgg acggctacat caatactaat ttgaccgcca acggcagcgt cggctggcag
     4021 ggtaaaaaca tcgctgccag cgggcggacc gatggcaacg ctggggtgat attcgacact
     4081 ggcctggaga acgatggtca gatcagcgcc aaaatcaacg ggcggatttt cccgcttaac
     4141 ggcaagcgta actatctccc gctctctccc tatggaagat atgaggtgga gttgcagaac
     4201 agcaaaaact cactcgacag ttacgatatc gtcagcggtc gcaaaagtca tctgactctc
     4261 tatccaggca atgtcgctgt cattgagcca gaggtgaagc agatggttac cgtctccggt
     4321 cgtatccgtg cggaagacgg cacactgctg gctaacgcac ggattaacaa ccatatcggc
     4381 cgaacccgaa ccgatgaaaa cggcgagttt gtcatggacg tggataagaa ataccccact
     4441 atcgattttc gctacagtgg caataaaacc tgcgaagtgg cactggaact caaccaggcg
     4501 cgcggtgccg tctgggtcgg tgatgtggtc tgcagcggcc tctcatcgtg ggcggcggtg
     4561 acgcagacag gagaagagaa tgagagttaa cctactgata gcgatgataa tctttgcgct
     4621 aatctggcct gcaaccgcgc tcagagcggc agtgagcaaa acaacctggg cggatgctcc
     4681 ggcacgcgag tttgtgtttg tcgaaaacaa ctcagacgac aactttttcg tcactcctgg
     4741 cggggcgctg gatccgcgcc tgaccggtgc caaccgctgg accggtttaa aatacaatgg
     4801 ttcaggaacc atctatcagc aaagcctcgg ctacattgat aacggttaca acaccggcct
     4861 ttataccaac tggaagtttg atatgtggct ggaaaattca ccagtttcat ctcctttaac
     4921 tggcttgcgc tgcatcaact ggtacgcagg gtgtaatatg accaccagtc ttatcctgcc
     4981 gcaaaccacc gacgccagtg gattttatgg cgcgacggtg accagcggcg gcgcgaaatg
     5041 gatgcacggc atgttgtcag acgcgtttta ccagtatctg caacaaatgc ccgtcggcag
     5101 cagctttaca atgaccatca atgcctgcca gacctctgtg aactatgacg ccagtagcgg
     5161 cgcacgctgt aaggatcagg cctccggcaa ctggtatgtt cgcaacgtca cccatacgaa
     5221 agcagcaaat ctgcggttga taaataccca ctcgctggcg gaagtattta tcaacagcga
     5281 cggagtaccg actctgggcg aagggaacgc cgactgccgg acgcaaacca tcggcagccg
     5341 ttcaggatta agttgtaaga tggttaacta taccctgcaa acaaacggac tcagcaacac
     5401 ctcaatccat atattcccgg cgatcgccaa ctcgtcgtta gcctcggccg tcggggcgta
     5461 cgatatgcag ttcagtctga atggcagttc atggaaaccg gtgagcaata ctgcctatta
     5521 ctacaccttc aacgagatga agagcgcaga ctcgatctat gttttcttct cgagcaactt
     5581 ctttaaacag atggtgaacc tcgggatcag cgatatcaac accaaagatc tattcaactt
     5641 tcgctttcag aacaccacat caccggagtc tggctggtat gaattttcta cctccaacac
     5701 gctgattatc aaaccccgtg atttcagcat cagtattatc tccgatgaat atactcagac
     5761 accgtcgcgg gagggatatg tcggcagcgg cgagtcggca ctcgatttcg gctatatcgt
     5821 aaccaccagc ggtaaaacag ctgccgacga agtgctgatc aaggtgaccg gacccgcgca
     5881 ggtgattggc gggcgctcct attgtgtctt cagctccgat gacggtaagg ctaaagtacc
     5941 gttcccggcg acgctttcct ttattacccg caacggagct acaaaaacct acgatgccgg
     6001 gtgcgatgat agctggcggg atatgaccga tgcgctgtgg ttgaccacac cgtggactga
     6061 tatctctggc gaagtggggc agatggataa gaccacagtc aaattttcga ttccaatgga
     6121 taacgccatt tctctgcgta cggtagatga taacggctgg tttggcgaag tcagcgcttc
     6181 aggagagatt catgttcagg cgacgtggcg taacattaac taaggccctg ctgacagtgg
     6241 tctgtatgct ggcggcacct ttgacacagg cgatttcggt cggcaatctg actttttcac
     6301 tgccgtccga gactgacttt gtcagcaaac gtgtcgtgaa taacaacaaa agcgcgcgga
     6361 tataccgtat tgccatcagt gctattgata gcccgggcag cagtgaattg cgcacccgac
     6421 cggtggatgg tgaactactt ttcgcccccc gccagctggc gttgcaggct ggtgagagcg
     6481 agtattttaa attttactat catggtccac gggataaccg cgagcgctac taccgggtct
     6541 catttcgcga ggtccccact cgtaacctga caaaacgcag cccaaccggc ggtgaggtca
     6601 gcacggagcc ggtggtggtg atggatacca ttctggtagt acgaccacgt caggttcagt
     6661 ttaaatggtc cttcgatcag gtgaccggaa cggtaagtaa caccggcaac acgtggttta
     6721 agctactgat taaaccagga tgtgattcga ccgaagagga aggcgatgcc tggtatctac
     6781 gtccgggaga cgtggttcat cagcctgagt tacgtcagcc ggggaatcat tatctggtct
     6841 ataacgacaa attcattaag attagcgatt cctgtccggc taagccccct tcggcggact
     6901 aagacttatc catcagacga gggaagaatt ccacctcaga gactcaaaaa tcgtttaaat
     6961 gatgtggaag cgatcgtcaa tgtcacctgt aacgatgagc tttctcatat gcacaccaca
     7021 gtgttcagga gacacggaaa ggtaccaggc ggcctgctaa cgagtggttt cttcactaat
     7081 taggacgtaa aatcggtaac ggctggaaat cattcaatac tcacactatc gaaagtacac
     7141 cagccaaccg cgatacgtcc ctgcatacga cgtaccgcag ttctctttac gatttatagc
     7201 tgtactggtg aattatgagc aatctgaatc catgcatgac gtgtggtgcc tgttgtgcat
     7261 ttttccgcgt ctctttttac tgggccgaag ccgacgatgc tggcggtacc attcccgcca
     7321 ggctcactga acaaatatcc ccttttcacc gatgcatgag cggtactaat cagaaaaatc
     7381 cccgatgtat tgcccttgca ggaacaccgg gcaaaaatgc ctgctgcacg atatatgaaa
     7441 atcgatcgtc cacatgcaga gaattcgcca tgtctggtga gaacggagaa gtcaatgagg
     7501 cttgcaatcg tgcaagagct aaatacgggc taacaccgtt ataaatatac aataattaat
     7561 tgcattgccc cgccagctgt aatagcgggg cttcgttttt cagggacaac aaaacctgaa
     7621 acttcttcca ttttccagca acaatgcctc ttcttctgta gcgttctccg gatctacatc
     7681 atcctgaagt agcgcgatat gatcactgac tgtccgcatc agaatatgaa aagcaacgtt
     7741 tttaacctat aaaaatggcg ctatatttgc gccattttta tcattcaaag cattatctat
     7801 ttgagcctaa agggatctca gggtctggct catgggtaat tctgtttcgt aaatctctgc
     7861 gaataatttc aatagaccag aaccagacta aatgtccaaa aatttcagag acattctcat
     7921 accacggaag atcaaagaga ggtggcgtaa gtcccatgag agggaatgaa atcatatgaa
     7981 caaaaagttg tgctaaagca cctgccagta agccctgcca aagtttaatt ttcggaaata
     8041 cttcggcgac cacacagtaa ccaacggcaa aaactatcga aaagattatg tgcgttacac
     8101 caacccagtt aaagacatgc ccggcgaagg tataaacagc cgaatttgga tctgtcagtc
     8161 ccaaccaatc ccgaagaaaa atgtacggag gattgagaaa gttacgggag caatcaattt
     8221 ggcctgcagc cctgattaat gattccggac cacacgctgc attaaacata tcaaccggac
     8281 tacgtggtgg caatggaact tctgcccccc acttaacaaa tgcggaaaca acgccagcaa
     8341 tcagcccaat gaatgcagca agaccataac gtctgcggtt tggtggagtt tgttcaaata
     8401 tattcatatc taccctgctt gtaccattat gttatacacc tctttaggag tattcataaa
     8461 acaaggcaaa tgtaaagaac tgtattgttt tgtataacca catggttacc taatcgccaa
     8521 ttaatataag ctccattatt tctccatatt tttatgttag aagtgacaag gagaaatg
//
LOCUS       DADSYM010000172         8554 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2415
            SAMN08039579-rid11723173.denovo.174, whole genome shotgun sequence.
ACCESSION   DADSYM010000172 DADSYM010000000
VERSION     DADSYM010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039579
            Sequence Read Archive: SRR6362533
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8554)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8554)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980162.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 03:39:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,484
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 4,347
            CDSs (with protein)               :: 4,347
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 43 of 85
            Pseudo Genes (incomplete)         :: 37 of 85
            Pseudo Genes (internal stop)      :: 19 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8554
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039579-rid11723173.denovo.174"
                     /strain="LGE2415"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8302 N 95.9628 W"
                     /collection_date="2015-08-24"
     gene            239..2617
                     /gene="ppsA"
                     /locus_tag="J1Y83_003847"
     CDS             239..2617
                     /gene="ppsA"
                     /locus_tag="J1Y83_003847"
                     /EC_number="2.7.9.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000069371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate synthase"
                     /protein_id="HBA7631950.1"
                     /translation="MSNNGSSPLVLWYNQLGMNDVDRVGGKNASLGEMITNLSGMGVS
                     VPNGFATTADAFNQFLDQSGVNQRIYELLDKTDIDDVTQLAKAGAQIRQWIIDTPFQP
                     ELENAIREAYAQLSADDENASFAVRSSATAEDMPDASFAGQQETFLNVQGFDAVLVAV
                     KHVFASLFNDRAISYRVHQGYDHRGVALSAGVQRMVRSDLASSGVMFSIDTESGFDQV
                     VFITSAWGLGEMVVQGAVNPDEFYVHKPTLAANRPAIVRRTMGSKKIRMVYAPTQEHG
                     KQVKIEDVPQEQRDIFSLTNEEVQELAKQAVQIEKHYGRPMDIEWAKDGHTGKLFIVQ
                     ARPETVRSRGQVMERYTLHSQGKIIAEGRAIGHRIGAGPVKVIHDISEMNRIEPGDVL
                     VTDMTDPDWEPIMKKASAIVTNRGGRTCHAAIIARELGIPAVVGCGDATERMKDGENV
                     TVSCAEGDTGYVYAELLEFSVKSSSVETMPDLPLKVMMNVGNPDRAFDFACLPNEGVG
                     LARLEFIINRMIGVHPRALLEFDDQEPQLQNEIREMMKGFDSPREFYVGRLTEGIATL
                     GAAFYPKRVIVRLSDFKSNEYANLVGGERYEPDEENPMLGFRGAGRYVSDTFRDCFAL
                     ECEAVKRVRNDMGLTNVEIMIPFVRTVDQAKAVVEELARQGLKRGENGLKIIMMCEIP
                     SNALLAEQFLEYFDGFSIGSNDMTQLALGLDRDSGVVSELFDERNDAVKALLSMAIRA
                     AKKQGKYVGICGQGPSDHEDFAAWLMEEGIDSLSLNPDTVVQTWLSLAELKK"
     gene            complement(2674..4374)
                     /gene="fadK"
                     /locus_tag="J1Y83_003848"
     CDS             complement(2674..4374)
                     /gene="fadK"
                     /locus_tag="J1Y83_003848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416216.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="medium-chain fatty-acid--CoA ligase"
                     /protein_id="HBA7631951.1"
                     /translation="MHPTGPHLGPDVLFRESKMKVTLTFNEQRRAAYRQQGLWGDASL
                     ADYWQQTARAMPDKIAVVDNHGATYTYSALDHAASSLANWMLAKGIESGDRIAFQLPG
                     WCEFTVIYLACLKTGAVSVPLLPSWREAELVWVLNKCQAKMFFAPTLFKQTRPVDLIL
                     PLQNQLPQLQQIVGVDKLAPATSSLSLSQILADNTPLTTAITTHGDELAAVLFTSGTE
                     GLPKGVMLTHNNILASERAYCARLNLTWQDVFMMPAPLGHATGFLHGVTAPFLIGARS
                     VLLDIFTPDACLALLEQQRCTCMLGATPFVYDLLNLLEKQPADLSALRFFLCGGTTIP
                     KKVARECQQRGIKLLSVYGSTESSPHAVVNLDDPLPRFMHTDGYAAAGVEIKVVDDAR
                     KTLPPGCEGEEASRGPNVFMGYFDEPELTARALDEEGWYYSGDLCRMDEAGYIKITGR
                     KKDIIVRGGENISSREVEDILLQHPKIHDACVVAMPDERLGERSCAYVVLKAPHHSLS
                     LEEVVAFFSRKRVAKYKYPEHIVVIEKLPRTVSGKIQKFLLRKDIMRRLTQDVCEEIE
                     "
     gene            complement(4377..4670)
                     /locus_tag="J1Y83_003849"
     CDS             complement(4377..4670)
                     /locus_tag="J1Y83_003849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HBA7631952.1"
                     /translation="MSQNATVNVDIKLGVNKFHVDEGHPHIILAANPDINEFRKLMKA
                     CPAGLYKQDDAGNIHFDSAGCLECGTCRVLCGNTILEQWQYPAGTFGIEFRYG"
     gene            complement(4667..5956)
                     /locus_tag="J1Y83_003850"
     CDS             complement(4667..5956)
                     /locus_tag="J1Y83_003850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HBA7631953.1"
                     /translation="MSDDKFDAIVVGAGVAGSVAALVMARAGLDVLVIERGDSAGCKN
                     MTGGRLYAHTLEAIIPGFAASAPVERKVTREKISFLTEESAVTLDFHREQPDVPQHAS
                     YTVLRNRLDPWLMEQAEQAGAQFIPGVRVDALVREGNKVTGVQAGDDILEANVVILAD
                     GVNSMLGRSLGMVSASNPHHYAVGVKEVIGLTPEQINDRFNVTGEEGAAWLFAGSPSD
                     GLMGGGFLYTNKDSVSLGLVCGLSDIAHAQKSVPQMLEDFKQHPAIRPLISGGKLLEY
                     SAHMVPEGGLAMVPQLVNDGVMIVGDAAGFCLNLGFTVRGMDLAIASAQAAATTVIAA
                     KERADFSASSLAQYKRELEQSCVMRDMQHFRKIPALMENPRLFSQYPRMVADIMNDMF
                     TIDGKPNQPVRKMIMGHAKKIGLINLLKDGIKGATVL"
     gene            complement(6011..6949)
                     /locus_tag="J1Y83_003851"
     CDS             complement(6011..6949)
                     /locus_tag="J1Y83_003851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit alpha"
                     /protein_id="HBA7631954.1"
                     /translation="MSQLNSVWVFSDNPERYAELFGGAQQWGQQVYAIVQNTEQAQAV
                     MPYGPKCIYVLEQNDALQRTENYAESIAALLKDKHPAMLLLAATKRGKALAARLSVQL
                     NAALVNDATAVDIVDGHICAEHRMYGGLAFAQEKINSPLAIITLAPGVQEPCTSDISH
                     QCPTETVPYVAPRHEILCRERRAKAASSVDLSKAKRVVGVGRGLAAQDDLKMVHELAA
                     VLNAEVGCSRPIAEGENWMERERYIGVSGVLLKSDLYLTLGISGQIQHMVGGNGAKVI
                     VAINKDKNAPIFNYADYGLVGDIYKVVPALISQLSR"
     gene            complement(6969..7733)
                     /locus_tag="J1Y83_003852"
     CDS             complement(6969..7733)
                     /locus_tag="J1Y83_003852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein"
                     /protein_id="HBA7631955.1"
                     /translation="MKIITCFKLVPEEQDIVVTPEYTLNFDNADAKISQFDLNAIEAA
                     SQLATDDDEIAALTVGGSLLQNSKVRKDVLSRGPHSLYMVQDAQLEHALPLDTAKALA
                     TAVEKIGFDLLIFGEGSGDLYAQQVGLLVGEILQLPVINAVSAIQRQGNTLVIERTLE
                     DDVEVIELSVPAVLCVTSDINVPRIPSMKAILGAGKKPVNQWQASDIGWSQSAPLAEL
                     TGIRVPPQTERKHIILDNDSPEAIAELAEHLKKALN"
     gene            8049..>8554
                     /locus_tag="J1Y83_003853"
     CDS             8049..>8554
                     /locus_tag="J1Y83_003853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HBA7631956.1"
                     /translation="MYQRCFDNASETLFVAGKTPRLSRFAFSDDPKWESGHHVHDNET
                     ELIYVKKGVARFTIDSSLYVAHADDIVVIERGRLHAVASDVNDPATTCTCALYGFQFQ
                     GAEENQLLQPHSCPVIAAGQGKEVIKTLFNELSVILPQSKNSQTSSLWDAFAYTLAIL
                     YYENFKNA"
BASE COUNT         2045 a         2194 c         2210 g         2105 t
ORIGIN      
        1 cttaaatttt attttttatg aaaacagcat ttcattttta tggtttcgtt tataccgatg
       61 gtttatgtgg aaattgtcga agagagcaga tttgcgcaac gctgggatca gtcttaaaaa
      121 gtaaaaaaat atatttgctt gaacgattca ccgttttttt catccggtta aatatgcaaa
      181 gataaatgcg cagaaatgtg tttctcaaac cgttcattta tcacaaaagg attgttcgat
      241 gtccaacaat ggctcgtcac cgctggtgct ttggtataac caactcggca tgaatgatgt
      301 agacagggtt gggggcaaaa atgcctccct gggtgaaatg attactaacc tttccggaat
      361 gggtgtttcc gttccgaatg gtttcgccac aaccgccgac gcgtttaacc agtttctgga
      421 ccaaagcggc gtaaaccagc gcatttatga actgctggat aaaacggata ttgacgatgt
      481 tacccagctt gcgaaagcgg gcgcgcaaat ccgccagtgg attatcgaca ctcccttcca
      541 gcctgagctg gaaaacgcca tccgcgaagc ctatgcacag ctttccgccg atgacgaaaa
      601 cgcttcgttt gcggtgcgtt cctccgctac tgcagaagat atgccggacg cttcttttgc
      661 cggtcagcag gaaactttcc tcaacgttca gggttttgac gccgttctcg tggcagtgaa
      721 gcatgtattt gcttctctgt ttaacgatcg cgccatctct tatcgtgtgc accagggtta
      781 cgaccatcgt ggcgtggcgc tctccgccgg tgttcagcgg atggtgcgct ccgacctcgc
      841 atcttctggc gtgatgttct ccattgatac cgaatccggc tttgaccagg tggtgtttat
      901 cacttccgca tggggccttg gtgaaatggt cgtgcagggt gcggttaacc cggatgagtt
      961 ttatgtgcat aaaccgacac ttgcggcgaa tcgcccggct atcgtgcgcc gcaccatggg
     1021 gtcgaaaaaa atccgcatgg tttacgcgcc gacccaggag cacggcaagc aggttaaaat
     1081 cgaagacgta ccgcaggaac agcgtgacat cttctcgctg accaacgaag aagtgcagga
     1141 actggcaaaa caggccgtac aaattgagaa acactacggt cgcccgatgg atattgagtg
     1201 ggcgaaagat ggccacaccg gcaaactgtt cattgtgcag gcgcgtccgg aaaccgtgcg
     1261 ctcacgcggt caggtcatgg agcgttatac gctgcattca cagggtaaga ttatcgccga
     1321 aggccgtgct atcggtcatc gcatcggcgc gggtccggtg aaagtcatcc atgacatcag
     1381 cgaaatgaac cgcatcgaac ctggcgacgt gctggttact gacatgaccg acccggactg
     1441 ggaaccgatc atgaagaaag catctgccat cgtcaccaac cgtggcggtc gtacctgtca
     1501 cgcggcgatc atcgctcgtg aactgggcat tccggcggta gtgggctgtg gagatgcaac
     1561 agaacggatg aaagacggtg agaacgtcac tgtttcttgt gccgaaggtg ataccggtta
     1621 cgtctatgcg gagttgctgg aatttagcgt gaaaagctcc agcgtagaaa cgatgccgga
     1681 cctgccgttg aaggtgatga tgaacgtcgg taacccggac cgagctttcg acttcgcctg
     1741 tctgccgaac gaaggcgtgg gacttgcgcg tctggaattt atcatcaacc gtatgattgg
     1801 cgtccaccca cgcgcgctgc ttgaatttga cgatcaagaa ccgcagttgc aaaacgaaat
     1861 ccgcgagatg atgaaaggtt ttgattctcc gcgtgaattt tacgttggtc gtctgactga
     1921 agggatcgcg acgctgggtg ccgcgtttta tccgaagcgc gtcattgtcc gtctctctga
     1981 ttttaaatcg aacgaatatg ccaacctggt cggtggtgag cgttacgagc cagatgaaga
     2041 gaacccgatg ctcggcttcc gtggcgcggg ccgctatgtt tccgacacct tccgcgactg
     2101 cttcgcgctg gagtgtgaag cagtgaaacg tgtgcgcaac gacatggggc tgactaacgt
     2161 tgagatcatg atcccgttcg tgcgtaccgt tgatcaggcg aaagcggtgg ttgaggaact
     2221 ggcgcgtcag gggctgaaac gtggcgagaa cgggctgaaa atcatcatga tgtgtgaaat
     2281 tccgtccaac gccttgctgg cagagcagtt cctggaatat ttcgacggct tctcaattgg
     2341 ctcaaacgac atgacgcagc tggcgctcgg tctggatcgt gactccggcg tggtgtctga
     2401 actgttcgat gagcgcaacg atgcggtgaa agcactgctg tcgatggcga ttcgtgccgc
     2461 gaagaaacag ggcaaatatg tcgggatttg cggtcagggt ccatccgacc acgaagactt
     2521 tgctgcatgg ttgatggaag aggggatcga tagcctgtct ctgaacccgg acaccgtggt
     2581 gcaaacctgg ttaagcctgg ctgaactgaa gaaataaaat aaatacccgg cggcgttttg
     2641 tcgccgggtt tatgtgatcc ccgaagatga aacttattca atctcttcac agacatcctg
     2701 agttaatcgc cgcataatat ctttccttaa caaaaacttt tgtattttac ctgagacagt
     2761 gcgcggtagt ttttcgatta ccacgatatg ttcaggatat ttatattttg cgacccgttt
     2821 acggctaaaa aaagccacca cctcttccag cgataatgaa tgatgcggcg ctttcagcac
     2881 gacataagcg catgaacgtt cacctaaacg ttcatcgggc atcgcaacca cacaggcatc
     2941 gtgaatttta ggatgctgca ataaaatatc ttctacttca cggctgctaa tattttcgcc
     3001 gccgcggaca ataatatctt tcttgcgccc ggttattttt atatagccag cctcatccat
     3061 gcggcagaga tcgccgctgt aataccagcc ttcttcatcc agcgcacggg cagttaattc
     3121 aggttcatca aaatacccca taaacacatt ggggccacgc gaggcttctt caccttcgca
     3181 acctggcggt aaagtcttgc gtgcgtcatc gaccacttta atctctacac ctgcggcagc
     3241 gtaaccgtcg gtgtgcataa agcgcggcaa aggatcgtcg agattcacca ccgcatgcgg
     3301 cgaactttct gtggaaccat aaacacttaa taatttaatg ccgcgctgct ggcattctcg
     3361 cgccactttt ttggggattg tggtacctcc gcaaagaaag aagcgcagcg ctgaaaggtc
     3421 tgcgggttgt ttctctagta aattcaaaag atcatagaca aacggcgttg cgccgagcat
     3481 acaggtgcaa cgctgctgct caagcagcgc gagacacgca tcaggagtga aaatatctaa
     3541 caacacgctg cgagcaccaa tcaaaaaagg tgctgttacg ccatgcagaa agcccgttgc
     3601 gtgaccaagt ggcgcaggca tcataaagac atcctgccag gtcagattta gccgcgcgca
     3661 ataagcccgc tcactggcga gaatattgtt atgcgttagc atcacaccct ttggcagacc
     3721 ctcggttccg gaggtaaata gcacagcggc taattcatcg ccgtgggtcg ttatcgccgt
     3781 ggtcagaggg gtattgtcgg cgagaatctg acttaatgag agggaagagg tggcgggagc
     3841 cagtttgtcc acgccgacaa tttgttgtag ttgtggaagc tgattttgca gcggcaggat
     3901 taaatctacc ggacgcgttt gtttaaacaa cgtcggtgca aagaacattt ttgcctgaca
     3961 cttattgagc acccacacca gttctgcttc ccgccaggaa ggtaacagcg gcacggaaac
     4021 cgcaccggtt ttcaggcagg caagatagat aacggtaaat tcacaccagc ctggcagttg
     4081 aaatgcgatg cgatcgcctg attcaatacc cttcgctaac atccagtttg ccagactgct
     4141 cgcggcgtga tcgagcgcgc tataggtgta cgttgcacca tgattatcga ccacggcaat
     4201 tttgtctggc atcgcacgag cggtctgctg ccagtaatcg gccagcgaag catcgcccca
     4261 taacccttgc tgacgatacg ccgcacgacg ttgttcgttg aacgttaatg tcactttcat
     4321 tttggactct cgaaacagaa catcaggccc gagatgcggg cctgtgggat gcatgattag
     4381 ccgtagcgaa attcaatacc gaaggtgcct gcgggatatt gccactgttc gagaatagtg
     4441 ttgccgcaca gcacccgaca ggtgccgcac tccagacaac cggcggaatc gaaatgaatg
     4501 tttcctgcgt catcctgctt ataaagtccg gcagggcaag ctttcattaa tttacggaat
     4561 tcattgatat cgggatttgc cgccaaaatg atatgcgggt ggccctcatc aacatggaat
     4621 ttattgacgc ctaatttgat gtcgacgtta accgtagcgt tctggctcat agcacggttg
     4681 ctcccttaat gccatctttc agcaagttga tcagcccaat tttcttcgcg tgtcccatga
     4741 tcattttgcg taccggctgg tttggtttgc cgtcaatggt gaacatatcg ttcatgatgt
     4801 cggcgaccat tcgtgggtat tggctaaaca ggcgcgggtt ttccatcagc gccgggatct
     4861 tgcggaaatg ctgcatatcg cgcataacac aactttgttc cagctcacgt ttgtattgcg
     4921 ccagactgct ggcggagaaa tccgcgcgtt ctttggcggc gatcaccgtt gtggcggcag
     4981 cctgagctga tgcaatggct aaatccatgc cgcgaactgt aaaacccaaa ttcaggcaga
     5041 agcctgcggc gtcaccaacg atcatcacgc catcgttaac cagctgcggc accattgcca
     5101 gaccgccctc cggcaccata tgcgcggaat attcaagcag tttgccgccg ctaatcagcg
     5161 ggcgaatggc ggggtgttgt ttaaaatctt ccagcatttg cggcacgctt ttttgcgcat
     5221 gggcgatatc actcaatcca caaaccagcc ccaacgatac ggaatctttg ttggtataga
     5281 ggaatccgcc gcccatcagg ccgtcagaag gggaaccggc aaacagccag gcggcacctt
     5341 cctcgcccgt aacattaaag cgatcgttga tctgttctgg tgtcaggcct ataacctctt
     5401 taacaccaac agcgtaatga tgcggattgg aagcggaaac cattcccagc gagcggccaa
     5461 gcatcgagtt aacgccatca gccagaatca ccacattcgc ttcgagaata tcatccccgg
     5521 cctggacgcc agtgaccttg tttccttcac gaaccagcgc atcgacgcgt acgcccggga
     5581 taaactgcgc gcccgcctgc tcggcttgtt ccatcaacca cgggtccaga cgattacgca
     5641 atacggtata agacgcgtgt tgcggaacat ctggttgctc gcggtgaaaa tcgagggtaa
     5701 cggcgctttc ttcggttaag aaggagattt tctcgcgtgt gaccttgcgt tctaccggcg
     5761 ctgatgctgc aaagcctgga ataattgctt caagtgtgtg ggcataaaga cgcccgccgg
     5821 tcatgttttt acatccggca ctgtcgccgc gttctatcac caggacatcc agcccggctc
     5881 gcgccatgac cagtgcggca acgctaccgg caacgcccgc accgaccaca atggcatcaa
     5941 atttgtcatc cgacatgcgt tatgctccct tcccgctgcg tttacagcgg gagatgtggt
     6001 tgaaagggaa ttagcgactc aactggctaa tcagggcagg gacgactttg tagatatcgc
     6061 ccaccagacc gtagtcggca tagttgaaga ttggcgcatt tttatcttta ttaatggcga
     6121 caatcacttt tgcgccgttg ccgccaacca tatgctggat ctgcccggag atccccagcg
     6181 tcaggtagag atcggatttc agcaacacgc cggagacacc gatataacgt tcacgctcca
     6241 tccagttctc gccttcggca attggacgtg aacagccgac ttcagcattc agtaccgccg
     6301 ccagttcgtg gaccattttc aggtcatcct gcgccgccag accacgaccg acgccaacca
     6361 cacgttttgc tttgctcagg tccacgctgc ttgccgcttt cgcacggcgt tcgcgacaga
     6421 gaatttcatg acgcggggca acataaggta ccgtttctgt cgggcactga tgagagatat
     6481 cactggtgca cggctcctga acaccgggtg caagggtaat gatcgccagc gggctgttga
     6541 tcttttcctg agcgaacgct aacccgccat acatccggtg ttcggcgcaa atgtgaccat
     6601 cgacaatatc caccgccgtg gcatcgttca ccagcgccgc attcagttgc acacttaacc
     6661 gtgctgccag agctttacca cgtttcgtcg cggccaacag caacatagcg gggtgtttat
     6721 ctttcagcag ggcagcaatg ctttcggcgt aattttcagt acgttgcagc gcgtcgtttt
     6781 gctcaagaac ataaatgcat tttggcccat aaggcataac tgcctgcgcc tgctcggtat
     6841 tttgtacaat ggcatacacc tgttggcccc attgctgagc gccgccaaac agttcagcat
     6901 aacgttcagg attatcgcta aagacccaga cgctgtttaa ttgactcatc gtttcttctc
     6961 cgtaggcttc agttcagggc tttcttcaga tgttccgcca gctcggcaat ggcctccggc
     7021 gaatcgttat cgagaatgat gtgcttacgt tctgtttgcg gcggtacgcg aatgccggtc
     7081 agttcagcaa gtggtgcgct ctggctccag ccaatatcac ttgcctgcca ctgatttacc
     7141 ggttttttac ccgcgccgag aatggctttc atcgaaggaa tacgtggcac gttaatatcg
     7201 gaggtgacgc agagcacggc tggaacagag agttcaataa cttcaacatc atcttcaagc
     7261 gtgcgttcaa tcaccagtgt attgccctga cgctgaatgg cactcactgc attaatcacc
     7321 ggaagttgca gaatttctcc gaccagcaag ccaacctgct gggcataaag gtcgccggaa
     7381 ccttcaccaa agatcagtaa atcgaagccg atcttttcaa ctgctgtcgc cagcgccttt
     7441 gcggtatcga gaggcagtgc atgctcaagt tgtgcatcct gcaccatata caggctgtgc
     7501 ggcccgcggg atagcacgtc tttgcgcact ttcgagttct gcaacaaaga gccgccgacg
     7561 gtcagcgcgg ctatctcatc gtcatctgtt gcaagctggc ttgcagcttc aatggcattg
     7621 agatcgaact ggctgatttt ggcgtcggca ttgtcgaaat tcagggtgta ttctggagtg
     7681 acaacaatgt cctgttcttc aggcaccagc ttaaagcagg ttattatttt catgatttct
     7741 cctgtgagtc gcatacggaa ccatctccga tggaaggtaa aaatggtctg aaaatagtgt
     7801 gcgatagcgg tgcggggttt tcgacggagc aagctgtttt ccttgccaac aatcttgcaa
     7861 aaaaaatgcg atcgctaaaa caaaagtgca atttgcggca atatagttat tccatgaccg
     7921 tgcaatcttg cattcagtgt aaataatctt gtttagctct cgctaaacgc ggttgtataa
     7981 ccgcaataca cgccgtattt tatccggcat atattgtttt tgtttgctca tggaataggg
     8041 aggttgtcat gtatcaacgc tgttttgata atgccagtga aacgctgttt gtcgccggta
     8101 aaacgccacg gctttcacgt ttcgcattta gcgatgatcc aaaatgggag tctggacatc
     8161 acgttcatga caatgaaacc gagctgattt acgtcaagaa aggggtagca aggtttacca
     8221 tcgattcttc gttatatgtc gcgcatgccg atgacattgt ggtgatagaa cgtggcaggc
     8281 tgcatgcggt ggcctctgac gttaacgatc cggcaacgac gtgtacctgt gcgctgtacg
     8341 gctttcagtt tcagggggct gaggaaaatc agctactgca accgcattct tgcccggtaa
     8401 ttgccgcagg gcagggaaaa gaagtcatta aaaccttatt taatgagcta agtgtgattt
     8461 tgccgcaaag taaaaatagc caaacatctt cgttatggga cgcatttgcc tatacgttag
     8521 caattcttta ctacgaaaac tttaaaaatg ctta
//
LOCUS       DADSYM010000173         8212 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2415
            SAMN08039579-rid11723173.denovo.175, whole genome shotgun sequence.
ACCESSION   DADSYM010000173 DADSYM010000000
VERSION     DADSYM010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039579
            Sequence Read Archive: SRR6362533
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8212)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8212)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980162.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 03:39:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,484
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 4,347
            CDSs (with protein)               :: 4,347
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 43 of 85
            Pseudo Genes (incomplete)         :: 37 of 85
            Pseudo Genes (internal stop)      :: 19 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8212
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039579-rid11723173.denovo.175"
                     /strain="LGE2415"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8302 N 95.9628 W"
                     /collection_date="2015-08-24"
     gene            82..912
                     /locus_tag="J1Y83_003854"
     CDS             82..912
                     /locus_tag="J1Y83_003854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000843961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HBA7631957.1"
                     /translation="MKSVNVPRKSSNLLKRRLKIAEIVASQGEIKVDDLSAQLGVSGV
                     TIRGDLSYLEQQGYLKRSFGGAIATPLSSEPEAIQPIAPLSLAQQMEIARHCARLIRD
                     RDTLFLGHGTICRKIIPLLSEVKKLRLITNDPEHALLANQFIDGEIILAGSEMLRPDT
                     ALAGKQLEQALQHFTITHSILEISHLDADGTLNINVPRLAAAWQLCFDHAQNKTVIVA
                     ADPAPSTAAIGHLHQAENVIVSRSINEQYQQQLQSADFVILYTSNECFTWSNRERLQE
                     "
     gene            944..1879
                     /locus_tag="J1Y83_003855"
     CDS             944..1879
                     /locus_tag="J1Y83_003855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001370872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HBA7631958.1"
                     /translation="MGELLAEFLSRNPHQKFTQPGEFIGPFPSGAPAIFAAQVAKLSH
                     RAIFFGCVGNDDFARLIIERLRHEGVITDGIHVMNNAVTGTAFVSYQNPQQRDFVFNI
                     PNSACGLFTAEHIDKDLLKQCNHLHIVGSSLFSFRMIDVMRKAITTIKSAGGTVSFDP
                     NIRKEMLSIPEMAQALDYLIEYTDIFIPSESELPFFARHKNLSEEQIVSDLLHGGVKH
                     VAIKRAQRGASYYKLKNGTLHAQHVAGHDIEIIDPTGAGDCFGATFITLFLSGFPAHK
                     ALQYANASGALAVMRQGPMEGISSLADIEDFLQQH"
     gene            1972..2214
                     /locus_tag="J1Y83_003856"
     CDS             1972..2214
                     /locus_tag="J1Y83_003856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhdT family protein"
                     /protein_id="HBA7631959.1"
                     /translation="MDTRFVQAHKEARWALGLTLLYLAVWLVAAYLPSVAPGFTGFPR
                     WFEMACILTPLLFIGLCWAMVKFIYRDIPLEDDDAA"
     gene            2204..3655
                     /gene="panF"
                     /locus_tag="J1Y83_003857"
     CDS             2204..3655
                     /gene="panF"
                     /locus_tag="J1Y83_003857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312157.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/pantothenate symporter"
                     /protein_id="HBA7631960.1"
                     /translation="MQLEVILPLVAYLVVVFGISVYAMRKRSTGTFLNEYFLGSRSMG
                     GIVLAMTLTATYISASSFIGGPGAAYKYGLGWVLLAMIQLPAVWLSLGILGKKFAILA
                     RRYNAVTLNDMLFARYQSRLLVWLASLSLLVAFVGAMTVQFIGGARLLETAAGIPYET
                     GLLIFGISIALYTAFGGFRASVLNDTMQGLVMLIGTVVLLIGVVHAAGGLSNAVDTLQ
                     TIDPQLVTPQGADDILSPAFMTSFWVLVCFGVIGLPHTAVRCISYKDSKAVHRGIIIG
                     TIVVAILMFGMHLAGALGRAVIPDLTVPDLVIPTLMVKVLPPFAAGIFLAAPMAAIMS
                     TINAQLLQSSATIIKDLYLNIRPDQMQNETRLKRMSAVITLVLGALLLLAAWKPPEMI
                     IWLNLLAFGGLEAVFLWPLVLGLYWERANAKGALSAMIVGGVLYAVLATLNIQYLGFH
                     PIVPSLLLSLLAFLVGNRFGTSVPQATVLTTDK"
     gene            3667..4548
                     /gene="prmA"
                     /locus_tag="J1Y83_003858"
     CDS             3667..4548
                     /gene="prmA"
                     /locus_tag="J1Y83_003858"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HBA7631961.1"
                     /translation="MPWIQLKLNTTGANAEDLSDALMEAGAVSITFQDTHDTPVFEPL
                     PGETRLWGDTDVIGLFDAETDMNDVVAILENHPLLGAGFAHKIEQLEDKDWEREWMDN
                     FHPMRFGERLWICPSWRDVPDENAVNVMLDPGLAFGTGTHPTTSLCLQWLDSLDLTGK
                     TVIDFGCGSGILAIAALKLGAAKAIGIDIDPQAIQASRDNAERNGVSDRLELYLPKDQ
                     PEEMKADVVVANILAGPLRELAPLISVLPVSGGLLGLSGILASQAESVCEAYADSFAL
                     DPVVEKEEWCRITGRKN"
     gene            4877..5842
                     /gene="dusB"
                     /locus_tag="J1Y83_003859"
     CDS             4877..5842
                     /gene="dusB"
                     /locus_tag="J1Y83_003859"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HBA7631962.1"
                     /translation="MRIGQYQLRNRLIAAPMAGITDRPFRTLCYEMGAGLTVSEMMSS
                     NPQVWESDKSRLRMVHIDEPGIRTVQIAGSDPKEMADAARINVESGAQIIDINMGCPA
                     KKVNRKLAGSALLQYPDVVKSILTEVVNAVDVPVTLKIRTGWAPEHRNCEEIAQLAED
                     CGIQALTIHGRTRACLFNGEAEYDSIRAVKQKVSIPVIANGDITDPLKARAVLDYTGA
                     DALMIGRAAQGRPWIFREIQHYLDTGELLPPLPLAEVKRLLCAHVRELHDFYGPAKGY
                     RIARKHVSWYLQEHAPNDQFRRTFNAIEDASEQLEALEAYFENFA"
     gene            5868..6164
                     /gene="fis"
                     /locus_tag="J1Y83_003860"
     CDS             5868..6164
                     /gene="fis"
                     /locus_tag="J1Y83_003860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001465625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator Fis"
                     /protein_id="HBA7631963.1"
                     /translation="MFEQRVNSDVLTVSTVNSQDQVTQKPLRDSVKQALKNYFAQLNG
                     QDVNDLYELVLAEVEQPLLDMVMQYTRGNQTRAALMMGINRGTLRKKLKKYGMN"
     gene            6250..7134
                     /gene="yhdJ"
                     /locus_tag="J1Y83_003861"
     CDS             6250..7134
                     /gene="yhdJ"
                     /locus_tag="J1Y83_003861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417728.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine-specific DNA-methyltransferase"
                     /protein_id="HBA7631964.1"
                     /translation="MRTGCEPTRFGNEAKTIIQGDALTELKKLPAESVDLIFADPPYN
                     IGKNFDGLIEAWKEDLFIDWLFEVIAECHRVLKKQGSMYIMNSTENMPFIDLQCRKLF
                     TIKSRIVWSYDSSGVQAKKHYGSMYEPILMMVKDAKNYTFNGDAILVEAKTGSQRALI
                     NYRKNPPQPYNHQKVPGNVWDFPRVRYLMDEYENHPTQKPEALLKRIILASSNPGDIV
                     LDPFAGSFTTGAVAVASGRKFIGIEINSEYIKMGLRRLDVASHYSAEELAKVKKRKTG
                     NLSKRSRLSEVDPDLIAK"
     gene            7218..7397
                     /locus_tag="J1Y83_003862"
     CDS             7218..7397
                     /locus_tag="J1Y83_003862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2556 domain-containing protein"
                     /protein_id="HBA7631965.1"
                     /translation="MIRKYWWLVVFAVFVFLFDTLLMQWIELLATETDKCRNMNSVNP
                     LKLVNCDELNFQDRM"
     gene            complement(7400..8062)
                     /gene="acrS"
                     /locus_tag="J1Y83_003863"
     CDS             complement(7400..8062)
                     /gene="acrS"
                     /locus_tag="J1Y83_003863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter transcriptional
                     repressor AcrS"
                     /protein_id="HBA7631966.1"
                     /translation="MAKRTKAEALKTRQELIETAIAQFAQHGVSKTTLNDIADAANVT
                     RGAIYWHFENKTQLFNEMWLQQPSLRELIQDYLTAGLEHDPFQQLREKLIVGLQYIAK
                     IPRQQALLKILYHKCEFNDEMLAEGVIREKMGFNPQTLREVLQACQQQGCVANNLDLD
                     VVMIIIDGAFSGIVQNWLMNMACYDLYKQAPALVDNVLRMFMPDENITKLIHKTNELS
                     VI"
BASE COUNT         2040 a         1972 c         2058 g         2142 t
ORIGIN      
        1 tcttttcgaa ttgtcgatat gattgaagga taactttcaa agaaaaatac aaggggctat
       61 agtgttacgc attaaaggcc aatgaagagt gttaacgtgc cgagaaaatc gtctaaccta
      121 ctgaaacgca gattaaaaat tgcagaaatt gttgcctccc agggtgaaat caaagttgat
      181 gacttatctg cgcaacttgg tgtctctggc gtgacgatcc gcggcgatct cagttatctg
      241 gagcaacagg gatatttaaa gcgatcattt ggcggtgcta ttgcgacacc actctcctct
      301 gaaccagaag cgatacaacc gatcgcgcca ttgtctctgg cacaacaaat ggaaatagca
      361 cgacattgcg cacgattaat tcgcgatcgt gacacgctat ttcttggaca tgggacaatc
      421 tgccgtaaga tcattccatt gctcagcgaa gtgaaaaaac tgcgtctgat caccaacgat
      481 cccgaacatg cgctgctggc aaatcagttt attgatggcg aaataatact ggctggcagt
      541 gaaatgctgc gccccgacac cgcactggcc ggaaaacagc ttgaacaggc acttcagcat
      601 ttcaccatta cacatagcat tctggaaatc agccatctgg atgctgatgg tactttaaat
      661 atcaacgttc ccagactcgc cgctgcctgg cagctctgtt tcgatcatgc ccagaataag
      721 acggttatcg ttgccgccga tcctgcgcca tcaacggctg ctatcgggca tttgcatcag
      781 gcagaaaatg tgatcgtcag ccgcagcatt aacgaacaat atcagcaaca attgcagtcg
      841 gctgatttcg ttatccttta taccagtaat gagtgcttta cctggtccaa tcgcgaaagg
      901 cttcaggaat gagaggggtt ttgtgacaat aactatctgc gctatgggcg aattgctggc
      961 cgagtttttg tcccgcaacc cacatcaaaa attcactcag cctggggagt ttatcgggcc
     1021 atttcccagc ggtgcgccag caatttttgc tgctcaggtg gcaaaactgt cccatcgggc
     1081 catcttcttt ggatgtgttg gtaatgatga ttttgcccga ctcattatag agcgtctccg
     1141 tcatgaaggt gtcattaccg atgggatcca tgttatgaac aatgccgtca caggtacggc
     1201 gttcgtgagt tatcaaaatc cccagcagcg ggatttcgtc tttaatatcc ctaacagcgc
     1261 ctgcggtttg tttactgccg agcacattga taaggatctg cttaaacagt gtaaccatct
     1321 gcatattgtg ggctcatcgt tgttctcatt tcgcatgatc gatgtcatgc gtaaagcaat
     1381 aacgacgatc aaatcggctg gcggcaccgt ttctttcgat cccaatattc gcaaagagat
     1441 gctgagtatt cctgaaatgg cgcaggctct cgattatttg attgaatata cggatatttt
     1501 tatccccagc gaaagcgaac tccctttctt cgcgcgtcac aaaaatctgt cagaagaaca
     1561 gattgttagc gatcttctcc acggcggcgt aaaacatgtg gcgataaaac gcgcccagcg
     1621 tggggccagc tattacaagc ttaaaaacgg tacattacac gcccagcatg ttgcaggtca
     1681 cgatatcgaa attatcgatc caacgggtgc aggcgactgc tttggcgcaa cgtttatcac
     1741 tcttttctta tccggtttcc cggcacacaa ggcgctgcaa tatgcaaatg ccagcggcgc
     1801 gctcgccgta atgcggcaag gtccgatgga agggatatcc tcactggcag acattgaaga
     1861 ctttttgcag cagcactgaa cggcaatttt ctgtcagatc attttccctg cctgaagact
     1921 tgccgtagaa ttgcccgttt ctcgtatccc cccatgagga ggaccccttc cgtggacact
     1981 cgttttgttc aggcccataa agaggcgcgc tgggcgctgg ggctgaccct tttgtatctg
     2041 gcggtatggt tagtagccgc ttacttacct agcgttgccc ccggttttac cggctttccg
     2101 cgctggtttg agatggcctg catcttgacg ccgctgctgt ttattggact gtgctgggcg
     2161 atggtgaaat ttatctatcg cgatatccca ctggaggatg acgatgcagc ttgaagtaat
     2221 tctaccgctg gtcgcctatc tggtggtggt gttcggtatc tctgtttatg cgatgcgtaa
     2281 acggagcact ggcaccttcc ttaatgagta tttcctcggc agccgctcta tgggcggtat
     2341 tgtgctggcg atgacgctca ccgcgaccta tatcagtgcc agttcgttta tcggcgggcc
     2401 tggagctgct tataaatacg gcctgggctg ggtattgcta gcgatgattc agcttcctgc
     2461 agtctggctt tcgctcggta ttctcggcaa gaagttcgca attcttgcgc gccgctacaa
     2521 tgcggtgacg ctgaacgata tgctgtttgc ccgctaccag agtcgtcttc tggtatggct
     2581 ggcgagtttg agtttgctgg ttgcgttcgt tggtgcgatg accgtgcagt ttatcggcgg
     2641 tgcgcgcctg ctggaaaccg cggcgggtat tccttatgaa accgggctgc tgattttcgg
     2701 tatcagcatt gcgttgtata ccgcctttgg cggctttcgc gccagcgtgc tgaacgacac
     2761 catgcaaggg ctggtgatgc tgattggcac cgttgtgctg cttattggcg tggtacatgc
     2821 cgcaggtggt ttaagtaatg cggtagacac gctgcaaacc atcgatccgc aactggttac
     2881 gccacaaggc gctgacgata ttctgtcgcc tgcctttatg acgtcgttct gggtactggt
     2941 gtgctttggc gttataggcc tgccgcatac tgcggtgcgc tgtatctctt ataaagacag
     3001 caaagccgta catcggggga tcattatcgg tacgattgta gtcgcaatcc tgatgttcgg
     3061 tatgcatctg gccggagcgt taggtcgggc ggtgatcccc gatctcaccg taccggatct
     3121 ggtgatccca acgttaatgg taaaagtgct gccaccgttt gctgccggga tcttcctggc
     3181 tgcgcctatg gctgcgatca tgtcgacaat taacgcccaa ctgctgcaaa gttccgctac
     3241 gatcattaaa gatctctatc tgaatatccg tccggatcaa atgcaaaacg agacgcgtct
     3301 gaagcggatg tcggcggtga tcacgttggt tctcggtgcg ttgctgctgc ttgccgcctg
     3361 gaagccgcca gaaatgatca tctggctaaa tttgctggcc ttcggtgggc tggaagccgt
     3421 tttcctgtgg ccgctggtgc tgggtcttta ctgggaacgc gccaacgcca aaggcgcgct
     3481 aagtgcgatg atcgttggcg gcgtgctgta tgccgtactc gcgacgctga atattcagta
     3541 cctgggcttc caccctatcg tgccctcgtt actactaagt ttgctggctt tcctggtcgg
     3601 aaaccgtttc ggtacatccg tcccgcaagc taccgttttg actactgata aataaagagt
     3661 tttgccatgc cttggatcca actgaaactg aacaccaccg gcgcaaacgc ggaagatctt
     3721 agcgatgcgc tgatggaagc gggtgccgtt tctatcactt ttcaggatac ccacgatacg
     3781 ccagtatttg agccgctgcc gggcgaaacg cgcctgtggg gcgacaccga tgtgattggt
     3841 ctgttcgacg ctgaaaccga tatgaacgac gtggtggcga ttctggaaaa ccatccgctg
     3901 ctcggcgcgg gcttcgcgca taaaatcgaa caactggaag ataaagactg ggagcgcgaa
     3961 tggatggata atttccaccc gatgcgcttt ggtgaacggc tgtggatctg cccaagctgg
     4021 cgtgatgtgc cggacgaaaa cgccgtcaac gtgatgttag acccagggct ggcgtttggt
     4081 acgggtaccc atccaactac ctctctgtgc ctgcaatggc tcgacagcct cgatttaacc
     4141 ggtaaaacgg tcatcgactt tggctgtggt tccggcattc tggctatcgc ggcgctgaag
     4201 ctgggtgcag caaaagccat tggtattgat atcgacccgc aggcgattca ggccagccgc
     4261 gacaacgccg aacgtaatgg cgtttctgac cgtctggaac tctacctacc gaaagatcag
     4321 ccagaagaaa tgaaagccga cgtggtggtc gctaacatcc ttgcaggccc attacgtgaa
     4381 ctggcaccgt taatcagcgt cctgccggtt tcaggtggct tgctgggcct ttccggtatt
     4441 ctggcaagcc aggcagagag cgtttgtgaa gcttatgccg acagcttcgc tctggacccg
     4501 gtcgtggaaa aagaagagtg gtgccgcatt accggtcgta agaattaacc ttcgcatcgc
     4561 cgtagggtga cgcaaggcga agtgcgagca aggtcacaaa aggcacgtta atttgccgat
     4621 tatttacgca aatttgcgtg ccaaaatttt cattcataaa gaaaaattga gaacttactc
     4681 aaatttcttg gagtgtaaat tttagtcact attttctaat ataatgattt ttatgagtaa
     4741 ttatcgcgcc aagctcattt taaatgcaat tctttgatcc atctcagatg attggtcaaa
     4801 gtttggcctt tcatctcgtg caaaaaatgc gtaatatacg ccgccttgca gtcacagtat
     4861 ggtcatttct taactcatgc gcatcggaca atatcagctc agaaatcgcc tgatcgcagc
     4921 gcccatggct ggcattacag acagaccttt tcggacgttg tgctacgaga tgggagccgg
     4981 attgacagta tccgagatga tgtcttctaa cccacaggtt tgggaaagcg acaaatctcg
     5041 tttacggatg gtgcacattg atgaacccgg tattcgcacc gtgcaaattg ctggtagcga
     5101 tccgaaagaa atggcagatg cagcacgtat taacgtggaa agcggtgccc agattattga
     5161 tatcaatatg ggttgcccgg ctaaaaaagt gaatcgcaag ctcgcaggtt cagccctctt
     5221 gcagtacccg gatgtcgtta aatcgatcct taccgaggtc gtcaatgcag tggacgttcc
     5281 tgttaccctg aaaattcgca ctggctgggc accggaacac cgtaactgcg aagagattgc
     5341 ccaactggct gaagactgtg gcattcaggc tctgaccatt catggccgta cacgcgcctg
     5401 tttgttcaat ggagaagctg agtacgacag tattcgggca gttaagcaga aagtttccat
     5461 tccggttatc gcgaatggtg acattactga cccgcttaaa gccagagctg tgctcgacta
     5521 tacaggggcg gatgccctga tgataggccg cgcagctcag ggaagaccct ggatctttcg
     5581 ggaaatccag cattatctgg acactgggga gttgctgccc ccgctgcctt tggcagaggt
     5641 taagcgcttg ctttgcgcgc acgttcggga actgcatgac ttttatggtc cggcaaaagg
     5701 gtaccgaatt gcacgtaaac acgtttcctg gtatctccag gaacacgctc caaatgacca
     5761 gtttcggcgc acattcaacg ccattgagga tgccagcgaa cagctggagg cgttggaggc
     5821 atacttcgaa aattttgcgt aaacagaaat aaagagctga cagaactatg ttcgaacaac
     5881 gcgtaaattc tgacgtactg accgtttcta ccgttaactc tcaggatcag gtaacccaaa
     5941 aacccctgcg tgactcggtt aaacaggcac tgaagaacta ttttgctcaa ctgaatggtc
     6001 aggatgtgaa tgacctctat gagctggtac tggctgaagt agaacagccc ctgttggaca
     6061 tggtgatgca atacacccgt ggtaaccaga cccgtgctgc tctgatgatg ggcatcaacc
     6121 gtggtacgct gcgtaaaaaa ttgaaaaaat acggcatgaa ctaattcagg ttagctaaat
     6181 gcttgattaa aaaggcgcta ctcggcatgg ggaagcgcct tttttatagg tgtcacaaag
     6241 ggagtgacca tgagaacagg atgtgaaccg acccgatttg gtaatgaagc aaagaccatc
     6301 atccagggtg atgcattaac cgaacttaaa aagctccccg ccgaaagtgt cgatctgatc
     6361 tttgccgacc caccgtataa catcggtaaa aattttgatg gtctgatcga agcctggaaa
     6421 gaagatctgt ttatcgactg gctgtttgaa gtgattgcag agtgccatcg cgttctgaaa
     6481 aagcagggca gcatgtacat catgaatagc acggaaaaca tgccctttat cgatctccag
     6541 tgccgcaagc tttttaccat caaaagtcgc atcgtctggt catatgacag ttctggagtg
     6601 caggcgaaaa aacactacgg ctccatgtac gaacccatcc tgatgatggt gaaagacgca
     6661 aagaactaca cgttcaacgg tgatgctatt ctggtagaag ctaaaaccgg atcgcagcgc
     6721 gcgttgatca attatcgcaa aaatcctcca cagccttaca atcatcaaaa agtaccgggt
     6781 aacgtctggg attttccgcg cgtgcgttat ttgatggatg aatatgaaaa ccacccgacg
     6841 caaaaaccgg aagccttgct gaaacgcatt attctcgcct cttccaaccc aggcgatatc
     6901 gttctcgacc cgtttgccgg tagctttact accggtgccg tagccgtcgc cagcgggcga
     6961 aaattcattg gtattgagat caacagcgag tacatcaaaa tggggcttcg acggctggat
     7021 gtcgcgtcgc attactctgc ggaagaactg gcgaaagtga aaaaaagaaa gaccggcaac
     7081 ctgtcaaaac gaagccggtt aagcgaagtt gaccccgatc tcattgcaaa gtaaaggaag
     7141 tgtaagcctg tttttcagat tattcatttc gtgtatattt cctgccagac ttggttaaac
     7201 atgcacaggc tcaggtaatg attcgcaagt attggtggct cgtcgttttc gctgtcttcg
     7261 tttttctgtt tgatacttta ctgatgcagt ggattgaact gctggcaaca gaaacagaca
     7321 aatgccgcaa tatgaactca gttaatccac taaaactggt taactgtgac gaactgaatt
     7381 ttcaggacag aatgtgaatt tatatgacac ttaattcatt cgttttatga attaatttcg
     7441 ttatgttttc atctggcatg aacattctta atacgttatc gaccagtgcg ggtgcttgtt
     7501 tataaaggtc ataacacgcc atattcatta accagttttg aacaattccg ctgaaggcac
     7561 catcaataat aatcatcaca acatctaaat cgagattatt tgctacacaa ccttgttgct
     7621 gacacgcctg caatacttcg cggagagtct gcggattaaa gcccatcttt tcgcgtatca
     7681 ctccctcggc cagcatctca tcattaaatt cacatttgtg atataagatt ttcagcaacg
     7741 cctgctggcg gggaattttg gcaatatatt gcaaaccgac aatcaatttt tcacgcaatt
     7801 gctgaaacgg gtcatgctct aacccagcgg tcaagtaatc ctggattaac tcccgcaatg
     7861 aaggctgttg caaccacatc tcattaaaca gttgagtctt gttttcgaag tgccagtaga
     7921 tagcgccacg cgtaacgtta gcagcgtcgg caatgtcgtt gagcgtcgtc ttgcttacgc
     7981 catgctgcgc aaactgggcg atggcagttt caatcagttc ttgccgggtc ttcagggctt
     8041 cagctttggt tctttttgcc atgattaatt attcaggaaa taaatatatt caacacagga
     8101 tgagaaaata gcgaaggtta atctatcacc taatgtgtat ttatacgaga ggctaatatt
     8161 gcgttgctat aaatcgtcaa ataaatgata tgtattattt acctaaaata ca
//
LOCUS       DADSYM010000174         8122 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2415
            SAMN08039579-rid11723173.denovo.176, whole genome shotgun sequence.
ACCESSION   DADSYM010000174 DADSYM010000000
VERSION     DADSYM010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039579
            Sequence Read Archive: SRR6362533
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8122)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8122)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980162.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 03:39:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,484
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 4,347
            CDSs (with protein)               :: 4,347
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 43 of 85
            Pseudo Genes (incomplete)         :: 37 of 85
            Pseudo Genes (internal stop)      :: 19 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8122
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039579-rid11723173.denovo.176"
                     /strain="LGE2415"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8302 N 95.9628 W"
                     /collection_date="2015-08-24"
     gene            567..1016
                     /gene="alaE"
                     /locus_tag="J1Y83_003864"
     CDS             567..1016
                     /gene="alaE"
                     /locus_tag="J1Y83_003864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-alanine exporter AlaE"
                     /protein_id="HBA7631967.1"
                     /translation="MFSPQSRLRHAVADTFAMVVYCSVVNMCIEVFLSGMSFEQSFYS
                     RLVAIPVNILIAWPYGMYRDLFMRAARKVSPSGWIKNLADILAYVTFQSPVYVAILLV
                     VGADWHQIMAAVSSNIVVSMLMGAVYGYFLDYCRRLFKVSRYQQVKA"
     gene            complement(1053..1397)
                     /locus_tag="J1Y83_003865"
     CDS             complement(1053..1397)
                     /locus_tag="J1Y83_003865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgaC family protein"
                     /protein_id="HBA7631968.1"
                     /translation="MYLRPDEVARVLEKVGFTVDVVTQKAYGYRRGENYVYVNREARM
                     GRTALVIHPTLKERSSTLAEPASDIKTCDHYQQFPLYLAGERHEHYGIPHGFSSRVAL
                     ERYLNGLFGEAS"
     gene            1549..1878
                     /locus_tag="J1Y83_003866"
     CDS             1549..1878
                     /locus_tag="J1Y83_003866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311560.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF883 domain-containing protein"
                     /protein_id="HBA7631969.1"
                     /translation="MFNRPNRNDVDDGVQDIQNDVNQLADSLESVLKSWGSDAKGEAE
                     AARSKAQALLKETRARMHGRTRVQQAARDAVGCADSFVRERPWCSVGTAAAVGIFIGA
                     LLSMRKS"
     gene            complement(1935..3269)
                     /locus_tag="J1Y83_003867"
     CDS             complement(1935..3269)
                     /locus_tag="J1Y83_003867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HBA7631970.1"
                     /translation="MPRYQDIARQLKTAIEQGELKPGARLPSSRTWSQELGVSRSTVE
                     NAYAELVAQGWLIRRGQAGTFVSERIYPQQSTVQVVAFAGESQQPLPFQMGLPALDLF
                     PRELWARVMGRRLRTQTRFDLALGDVCGEAALREAIVDYLRVSRGIDCQPEQVFITHG
                     YAASIALILHALAKPGNGMWIEDPGFPLIRPIVTRHDVEILPVPVDDNGLDITSGIQN
                     YPDARFALITPAHQSPLGVALSLARRHQILEWADRSQAWIIEDDYDSEFRYHGKPLPA
                     LKSLDAPQRVIYAGTFSKALFPALRCAWLVVPVKQIAQFRHQASLAPCAVPVLWQNTL
                     ADFLREGHFWRHLKKMRQHYAQRRQWIEQALTQQGFQVVPQKGGIQMVIRMIGDDIAH
                     ARKANAAGLAVQALSDWRIRSSGEGGLLLSFTNIVNEGMARQVAQQLRKALS"
     gene            3357..3788
                     /locus_tag="J1Y83_003868"
     CDS             3357..3788
                     /locus_tag="J1Y83_003868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HBA7631971.1"
                     /translation="MTTLRQPYYELSPAVYNALVQAKTALENSTLDTTLMELVYLRVS
                     QINGCAFCLEMHSKALRKSGVPQHKLDALAGWRVSHHFDERERAALAWAESVTEIART
                     HAEDEVYQPLLEHFSAAEISDLTFAIGLMNCFNRLAVSMRM"
     gene            3997..4242
                     /gene="nrdH"
                     /locus_tag="J1Y83_003869"
     CDS             3997..4242
                     /gene="nrdH"
                     /locus_tag="J1Y83_003869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HBA7631972.1"
                     /translation="MRITIYTRNDCVQCHATKRAMENRGFDFEMINVDRVPEAAEALR
                     AQGFRQLPVVIAGDLSWSGFRPDMINRLHPAPHAASA"
     gene            4239..4649
                     /gene="nrdI"
                     /locus_tag="J1Y83_003870"
     CDS             4239..4649
                     /gene="nrdI"
                     /locus_tag="J1Y83_003870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HBA7631973.1"
                     /translation="MSQLVYFSSSSENTQRFIERLGLPAVRIPLNERERIQVDEPYIL
                     IVPSYGGGGTAGAVPRQVIRFLNDEHNRALLRGVIASGNRNFGEAYGRAGDVIARKCG
                     VPWLYRFELMGTQSDIENVRKGVTEFWQRQPQNA"
     gene            4622..6766
                     /gene="nrdE"
                     /locus_tag="J1Y83_003871"
     CDS             4622..6766
                     /gene="nrdE"
                     /locus_tag="J1Y83_003871"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HBA7631974.1"
                     /translation="MATTTAERLTQETMDYHALNAMLNLYDSAGRIQFDKDRQAVDAF
                     MTTHVRPNSVAFSSQQQRLNWLVNEGYYDESVLNRYSRDFVITLFAHAHASGFRFQTF
                     LGAWKFYTSYTLKTFDGKRYLEDFADRVTMVALTLAQGDETLATQLTDEMLSGRFQPA
                     TPTFLNCGKQQRGELVSCFLLRIEDNMESIGRAVNSALQLSKRGGGVAFLLSNLREAG
                     APIKRIENQSSGVIPVMKMLEDAFSYANQLGARQGAGAVYLHAHHPDILRFLDTKREN
                     ADEKIRIKTLSLGVVIPDITFHLAKENAQMALFSPYDVERVYGKPFADIAISEHYAEL
                     VTDERIRKKYLNARDFFQRLAEIQFESGYPYIMYEDTVNRANPIAGRINMSNLCSEIL
                     QVNSASEYDENLDYARTGHDISCNLGSLNIAHTMDSPDFARTVETAVRGLTAVSDMSH
                     IRSVPSIEAGNAASHAIGLGQMNLHGYLAREGIAYGSPEALDFTNLYFYTITWHALRT
                     SMLLARERGETFAGFKQSRYASGEYFSQYLQGNWQPKTAKVGELFARSGITLPTREMW
                     VQLRDDVMRYGIYNQNLQAVPPTGSISYINHATSSIHPIVAKVEIRKEGKTGRVYYPA
                     PFMTNENLALYQDAYEIGAEKIIDTYAEATRHVDQGLSLTLFFPDTATTRDINKAQIY
                     AWRKGIKTLYYIRLRQMALEGTEIEGCVSCAL"
     gene            6776..7735
                     /gene="nrdF"
                     /locus_tag="J1Y83_003872"
     CDS             6776..7735
                     /gene="nrdF"
                     /locus_tag="J1Y83_003872"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HBA7631975.1"
                     /translation="MKLSRISAINWNKISDDKDLEVWNRLTSNFWLPEKVPLSNDIPA
                     WQTLSVVEQQLTMRVFTGLTLLDTLQNVIGAPSLMPDALTPHEEAVLSNISFMEAVHA
                     RSYSSIFSTLCQTKDVDAAYAWSEENAPLQRKAQIIQQHYRGDDPLKKKIASVFLESF
                     LFYSGFWLPMYFSSRGKLTNTADLIRLIIRDEAVHGYYIGYKYQKNMEKISQAQREEL
                     KSFAFDLLLELYDNELQYTDELYAETPWADDVKAFLCYNANKALMNLGYEPLFPAEMA
                     EVNPAILAALSPNADENHDFFSGSGSSYVMGKAVETEDEDWNF"
BASE COUNT         2091 a         2049 c         1941 g         2041 t
ORIGIN      
        1 tgcacgagat tatttcagct gttattttct gaaactctca gaataatttc aagattatat
       61 attagccatt ttgatgagta atttcggtta ttggtgaaca gagctgctct ctctgctcga
      121 taattccgca agcctgacaa aaccaaagtg aataaaaatg caactcaacc gaataaaaga
      181 aaacatctat cactggtgct ttctgctgtc aatgtacatt atctggcata aaaaatgctg
      241 aaaatgaccc ggtcactaca tacaccgaat tctctacgct tatagcgtta cctcaccccc
      301 aaaccttcat gagtgaaaat aaaggtaaac acatatccaa ttgatttaat taaaaataaa
      361 tcataccggc atgtaaaaat ccacaatgta caaaaaacgc gccgttacgg aatattttat
      421 ctacaaaaac agattaaata aaaatttttc actaattgat tagtcatagc cagcgatata
      481 cgctatgcga aaatgcagat agcaatgaga tccactgctt tcatctccat taacatccca
      541 ttacgctttt attaaggagc attagcatgt tctcaccgca gtcacgcttg cgtcatgcag
      601 ttgcagatac gttcgcgatg gttgtttact gttctgtcgt gaacatgtgt attgaagttt
      661 tcctctccgg aatgagcttc gaacagtctt tttattccag attggtagcg attccggtga
      721 acatcttaat tgcatggcca tacggtatgt accgcgatct gtttatgcgc gcggcacgca
      781 aagttagccc gtcgggctgg ataaaaaatc tggcggatat cctggcttat gtgacgttcc
      841 agtcaccggt gtatgtggcg atcttgttag tggtgggcgc agactggcat cagattatgg
      901 cggcggtcag ttcaaacatc gttgtttcga tgttgatggg ggcggtttat ggctacttcc
      961 tcgattattg ccgccgactg tttaaagtca gccgttacca gcaggtaaaa gcctgaccct
     1021 tcttttcgcg actggcatcg ccagtcgcgt ctttaactgg cttcgccaaa taaaccattc
     1081 aaataacgtt caagcgcaac acgcgaacta aagccatgcg ggataccgta atgctcatga
     1141 cgttcacccg ctaaatagag cggaaattgc tggtaatgat cgcaagtttt aatatcggaa
     1201 gcgggttcag ccagtgttga actacgttct tttaatgttg gatgaatcac cagggcggta
     1261 cgtcccattc gcgcttcgcg attaacatag acataatttt ccccacggcg gtaaccatac
     1321 gctttctggg ttaccacatc gacagtaaaa ccgacttttt caagtacgcg cgccacctcg
     1381 tctggtcgta aatacatatt tgatcctcgt tattatcaac cgcgggccta ccttacctga
     1441 ttgcgcattc acaacgcttt cagaaaagtc cataaagccg cgatgaacct cagttaagaa
     1501 aattatggtc tacactgaaa attacatcga attctaatgg gggatcatat gtttaaccga
     1561 ccgaaccgca acgacgttga tgatggcgtg caggatatcc agaatgatgt caatcaatta
     1621 gctgacagtc tcgaatctgt attgaagtcc tggggcagcg acgccaaagg ggaagccgaa
     1681 gccgctcgta gtaaagcgca ggctctactg aaagaaacca gggcgcgaat gcatggtcgt
     1741 actcgcgtcc agcaagccgc gcgcgatgcc gttggctgcg cggattcttt tgttcgtgaa
     1801 agaccctggt gtagtgtggg tacagcggca gcggtgggta tttttatcgg cgcactgtta
     1861 agcatgcgca aatcgtagtg caaaaatgat aataaatacg cgtctttgac cccgaagcct
     1921 ggcttcgggg ttttttagct taaggcttta cgtaattgtt gtgctacctg tcgcgccata
     1981 ccttcgttaa cgatattagt aaacgaaagc agtaatccac cttccccact tgaacggata
     2041 cgccagtcgc taagtgcctg caccgcaagg cctgcagcat tggctttacg cgcatgggca
     2101 atatcatcgc ctatcattct gatcaccatc tggataccac ctttctgcgg cacaacctga
     2161 aatccttgct gcgtcaacgc ttgctcaatc cattgccgac gttgagcata atgctgacgc
     2221 attttcttca gatgccgcca gaaatgcccc tcgcgaagaa agtctgccag tgtgttctgc
     2281 catagaacag gtaccgcaca tggagccagt gacgcctggt ggcggaattg tgcaatttgc
     2341 ttcaccggca ccaccagcca cgcacagcgc aatgcaggaa atagcgcttt gctgaatgtt
     2401 ccggcataaa ttacccgctg cggtgcgtcg agacttttta acgccggtaa cggcttaccg
     2461 tgatagcgaa actcactgtc ataatcatct tcaataatcc atgcctgact acgatctgcc
     2521 cattccagta tctgatgcct gcgcgctaaa gagagcgcca cacccagcgg actttggtgt
     2581 gctggtgtta tcagggcaaa acgcgcatca ggataatttt gtattccgct tgtgatatcc
     2641 agtccattgt catcaaccgg cacaggcaaa atttccacat catggcgagt gacaatcggg
     2701 cgaatcagtg gaaagccggg atcttctatc cacatcccgt ttcccggttt cgccagcgcg
     2761 tgcagaatta aagctattga ggccgcataa ccgtgagtga taaagacctg ctctggctga
     2821 caatcaatcc cacgtgaaac gcgtaagtaa tcaactattg cctcgcgcaa cgccgcctca
     2881 ccgcagacat cgcctaacgc caaatcaaag cgcgtctggg tacgaaggcg acgccccatc
     2941 acccgcgccc acagctctcg cggaaagaga tcgagtgcgg gtaatcccat ttgaaatggc
     3001 aacggttgct gactttcacc ggcaaaagct acaacttgta cagtagattg ttgcggatat
     3061 atccgctcac tgacaaatgt gccagcctgt ccccgcctga tcagccatcc ttgcgccacc
     3121 agctcagcat acgcattttc gacagtggat cgagacaccc ccagctcctg cgaccaggta
     3181 cgacttgaag gcagccttgc gccgggtttc agttcccctt gctcaatagc cgtttttaac
     3241 tgacgggcga tatcctgata gcgcggcata tggtcttctt ttttaggtaa tttatggctc
     3301 tctttagcag tccattatag tgctacattg cgggcgttaa taagaggaga aactgtatga
     3361 ctacattacg tcagccttac tacgaactta gcccggcagt gtataacgca ctagtgcagg
     3421 ccaaaacggc actggaaaat agcacgctgg ataccacgtt gatggagtta gtttatttgc
     3481 gcgtctcgca aatcaacggc tgcgcatttt gtctggagat gcacagcaaa gcattgcgca
     3541 aatccggcgt gccacagcac aaactggacg ccctggcagg ttggcgcgta agccatcatt
     3601 ttgatgaacg cgagcgggcg gcgctggcat gggcggaatc ggtaaccgaa attgccagaa
     3661 cccatgcgga agacgaggtt tatcagcctt tgcttgagca tttcagcgca gcagagatca
     3721 gcgacttaac gtttgccatc gggctgatga attgttttaa ccgtctggcc gtttccatgc
     3781 ggatgtaact tcttgatgcc gggtatttgc ccggcaaaaa aacacaaaat cccctacccc
     3841 gttgagccat tcatcggtgg cgtaatcaat ctaaaattcc catatcttgt gtggttgaat
     3901 cttttttcaa ctacatctag tatctctgta tcaacagaga gacaacccga cgcgtatcat
     3961 cgcgccgtat cttcatttta aacggaaata cgaatcatgc gcattactat ttacactcgt
     4021 aacgattgcg tccagtgcca cgccaccaaa cgggcgatgg aaaaccgggg ctttgatttt
     4081 gaaatgatta atgtcgatcg cgttcctgaa gcggcagaag cgctgcgtgc tcagggcttt
     4141 cgtcagttgc cggttgtgat tgcaggcgat cttagctggt ctggtttccg cccggacatg
     4201 atcaatcgtc tgcatccagc gccacacgcg gccagtgcat gagccagctc gtctactttt
     4261 ccagcagctc cgaaaatacg cagcgtttta tcgaacgttt agggctgccc gcggtgcgca
     4321 tcccgctcaa tgagcgggaa cggattcagg tagacgagcc ttacatcctg atcgtgccct
     4381 cttacggcgg cggcggtacg gctggcgcgg tgccacgaca ggtcattcgc tttttaaacg
     4441 acgagcataa ccgggcgttg ctgcgcggcg ttattgcttc gggtaatcgc aactttggtg
     4501 aggcatatgg ccgcgccgga gatgtgattg cccggaaatg cggcgtgccg tggctgtacc
     4561 gctttgaact catgggtacg caaagcgata tcgaaaacgt tcgtaaagga gtaaccgaat
     4621 tttggcaacg acaaccgcag aacgcctgac gcaggaaacg atggattatc acgctctgaa
     4681 tgcgatgctt aacctttacg atagcgcagg tcgcattcag ttcgataaag atcgccaggc
     4741 agtagacgcc tttatgacga cgcatgtgcg tccgaacagt gtggccttca gtagccagca
     4801 gcagcgcctg aactggctgg ttaacgaagg ttactatgat gaaagcgtcc ttaatcgcta
     4861 ctctcgcgat tttgtcatta cgctgtttgc ccacgcgcac gccagcggtt ttcgtttcca
     4921 gacattcctc ggagcatgga agttttacac cagctatacg ttgaagacat tcgacggtaa
     4981 gcgttatctg gaagattttg ccgatcgcgt cacgatggtg gcgctgacgc tggcacaagg
     5041 cgatgaaaca ctggcgacgc agctcactga tgagatgctg tcaggacgct ttcagccagc
     5101 cacgccaaca ttcctcaact gcggtaagca gcagcgcggc gaactggttt cctgttttct
     5161 gctgcgtatt gaagacaata tggagtcgat tggccgggca gtgaattccg cactacaact
     5221 gtcgaaacgc ggcggcggcg tagcattttt gctgtcgaat ctgcgggaag cgggcgcgcc
     5281 aattaaacgt attgaaaatc aatcttctgg cgtgattccg gtgatgaaaa tgctggaaga
     5341 cgcattttct tatgccaacc agctcggcgc gcgtcagggt gctggcgcag tctatttaca
     5401 tgcccatcat cccgatattc tgcgttttct cgatacaaag cgggaaaatg ccgacgagaa
     5461 aatccgcatt aaaacactgt cgctgggcgt ggtgatcccg gatatcactt tccatctggc
     5521 aaaagagaat gcgcagatgg cactgttttc gccttatgac gttgagcggg tttatggcaa
     5581 gccgtttgct gatatcgcca tcagtgaaca ctatgccgaa ctggttaccg atgaacgcat
     5641 ccgcaaaaaa tacctcaacg cccgtgattt cttccagcga ctggcagaaa tccagtttga
     5701 gtccggctat ccctacatca tgtatgaaga cacggtaaac cgtgctaacc ctatcgccgg
     5761 gcgcataaat atgagcaatc tctgctcaga aattttgcag gttaacagcg cctcagagta
     5821 tgacgagaat ctcgactatg cccgcacagg ccatgatatt tcctgcaatc tagggtcgtt
     5881 gaatattgcc cacaccatgg attcccccga ctttgcccgc acggtagaga ctgccgtacg
     5941 cggtttaacg gcggtgtcag atatgagtca tatccgcagc gtgccgtcca tcgaagccgg
     6001 aaatgccgcc tcacacgcca tcggtctggg gcagatgaat ttacacggtt atctggcacg
     6061 agaaggcatc gcttatgggt cgccggaagc actggatttc accaatctct atttctatac
     6121 catcacctgg cacgcactgc gtacctcgat gttgctggcg cgcgaacgcg gtgaaacctt
     6181 cgccgggttc aaacagtcac gctatgccag tggtgaatat ttcagccaat atctgcaagg
     6241 taactggcag ccgaaaacgg cgaaagttgg cgaactgttt gcccgtagcg gtattacgtt
     6301 acctacccgt gagatgtggg tgcagctgcg cgacgacgtg atgcgctatg gcatatacaa
     6361 ccagaatctc caggcggtgc cgccaaccgg ttctatctct tatatcaacc atgccacgtc
     6421 gagtattcat ccgattgtgg cgaaagtaga gatacgcaaa gagggcaaaa caggacgcgt
     6481 ttactaccct gccccgttta tgactaacga aaatctggcg ctgtatcagg acgcttacga
     6541 aatcggcgca gaaaagatca tcgacaccta cgcggaagcg actcgccatg tcgatcaggg
     6601 gctatcgctg acgctgtttt tccccgatac cgccaccact cgcgatatca acaaagcgca
     6661 gatttacgcc tggcgcaagg gtatcaaaac gctctattac attcgcctgc gtcagatggc
     6721 gctggaaggc actgaaattg aaggctgcgt ctcctgtgca ctttaaggaa tatctatgaa
     6781 actctcacgt atcagcgcca tcaactggaa caagatatct gacgataaag atctggaggt
     6841 gtggaatcgc ctgaccagca atttctggct accagaaaag gtgccactgt cgaacgatat
     6901 acctgcatgg cagacattaa gtgtcgtaga acaacaacta acaatgcgcg tttttactgg
     6961 cctgacgctg ctcgacacgc tgcaaaatgt tatcggcgcg ccctcgctga tgcccgatgc
     7021 actcaccccc catgaagagg cggtattatc aaatatcagc tttatggaag cggttcatgc
     7081 ccgctcttac agttcaattt tctcgacgct gtgccagacc aaagatgtcg atgccgccta
     7141 cgcctggagt gaagaaaacg cgccgttgca gcgaaaagca cagattattc agcaacatta
     7201 tcgcggtgat gatccgctga aaaagaaaat cgccagtgtg tttcttgaat cttttttgtt
     7261 ctattccggt ttctggctgc cgatgtattt ttccagccgc ggaaagctaa ccaataccgc
     7321 agacctgatc cgtctgatta tccgcgatga agcagtccac ggttattaca tcggctataa
     7381 atatcagaaa aacatggaaa agatctccca ggcacaacgt gaagagttga agagtttcgc
     7441 cttcgatttg ctactggaac tttatgacaa cgagttgcaa tacaccgatg agttgtacgc
     7501 cgaaaccccg tgggctgacg atgtgaaagc gtttctctgt tacaacgcca ataaagcgtt
     7561 gatgaatctg ggctacgaac cgctatttcc cgcagaaatg gcggaagtga atccggcaat
     7621 cctcgccgcg ctttcgccga atgccgatga aaatcacgat ttcttttccg gttcaggctc
     7681 ttcatatgtg atgggaaaag cggtcgaaac agaagatgaa gactggaatt tctgaggggg
     7741 ttattttcaa aaatatcact acccgctgca gggaaataat tcccgccaaa tagctttata
     7801 tcacgtaaat aatttgtggt gatctacact gatactctgt tgcattattc gcctgaaacc
     7861 acaatattca ggcgtttttt tgctatcttt gacaaaaaat atcaactttt ctcgatttgc
     7921 tctcagccct tatatcatgg gaaattccgg cgatttgctc acatcaatat tcatgccaca
     7981 tttgccatca ggggttgcct cagattctca gtatgttagg gtagaaaaaa gtgactattt
     8041 ccattgggta atgtatcgac ataaacaaat aacaggaatc tttctattgc atggcaatta
     8101 aattagaaat taaaaatctt ta
//
LOCUS       DADSYM010000175         8102 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2415
            SAMN08039579-rid11723173.denovo.177, whole genome shotgun sequence.
ACCESSION   DADSYM010000175 DADSYM010000000
VERSION     DADSYM010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039579
            Sequence Read Archive: SRR6362533
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8102)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8102)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980162.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 03:39:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,484
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 4,347
            CDSs (with protein)               :: 4,347
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 43 of 85
            Pseudo Genes (incomplete)         :: 37 of 85
            Pseudo Genes (internal stop)      :: 19 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8102
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039579-rid11723173.denovo.177"
                     /strain="LGE2415"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8302 N 95.9628 W"
                     /collection_date="2015-08-24"
     gene            87..1064
                     /gene="csiD"
                     /locus_tag="J1Y83_003873"
     CDS             87..1064
                     /gene="csiD"
                     /locus_tag="J1Y83_003873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311547.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation induced protein CsiD"
                     /protein_id="HBA7631976.1"
                     /translation="MNALTAVQNNAVDSGQDYSGFTLIPSAQSPRLLELTFTEQTTKQ
                     FLEQVAEWPVQALEYKSFLRFRVGKILDDLCANQLQPLLLKTLLNRAEGALLINAVGI
                     DDVAQADEMVKLATAVAHLIGRSNFDAMSGQYYARFVVKNVDNSDSYLRQPHRVMELH
                     NDGTYVEEITDYVLMMKIDEQNMQGGNSLLLHLDDWEHLDHYFRHPLARRPMRFAAPP
                     SKNVSKDVFHPVFDVDQQGRPVMRYIDQFVQPKDFEEGVWLSELSDAIETSKGILSVP
                     VPVGKFLLINNLFWLHGRDRFTPHPDLRRELMRQRGYFAYATNHYQTHQ"
     gene            1084..2352
                     /gene="lhgO"
                     /locus_tag="J1Y83_003874"
     CDS             1084..2352
                     /gene="lhgO"
                     /locus_tag="J1Y83_003874"
                     /EC_number="1.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311548.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-2-hydroxyglutarate oxidase"
                     /protein_id="HBA7631977.1"
                     /translation="MYDFVIIGGGIIGMSTAMQLIDVYPDARIALLEKESGPAFHQTG
                     HNSGVIHAGVYYTPGSLKAQFCLAGNRATKAFCDQNGIRYDNCGKMLVATSELEMERM
                     RALWERTAANGIEREWLKAEELREREPNITGLGGIFVPSSGIVSYREVTAAMAKIFQA
                     RGGEIIYNAEVSALSEHKNGVVIRTRQGGEYEASTLISCSGLMADRLVKMLGLEPGFI
                     ICPFRGEYFRLAPEHNQIVNHLIYPIPDPAMPFLGVHLTRMIDGSVTVGPNAVLAFKR
                     EGYRKRDFSFSDTLEILGSSGIRRVLQNHLRSGLGEMKNSLCKSGYLRLVQKYCPRLS
                     LSDLQPWPAGVRAQAVSPDGKLIDDFLFVTTPRTIHTCNAPSPAATSAIPIGAHIVSK
                     VQTLLASQSNPGRTLRAARSVDALHAAFNQ"
     gene            2375..3823
                     /gene="gabD"
                     /locus_tag="J1Y83_003875"
     CDS             2375..3823
                     /gene="gabD"
                     /locus_tag="J1Y83_003875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent succinate-semialdehyde
                     dehydrogenase"
                     /protein_id="HBA7631978.1"
                     /translation="MKLNDSNLFRQKALINGEWLDANNGEVIDVTNPANGDKLGSVPK
                     MGADETRAAIDAANRALPAWRALTAKERANILRNWFNLMMEHQDDLARLMTLEQGKPL
                     AEAKGEISYAASFIEWFAEEGKRIYGDTIPGHQADKRLIVIKQPIGVTAAITPWNFPA
                     AMITRKAGPALAAGCTMVLKPASQTPFSALALAELAIRAGVPAGVFNVVTGSAGAVGN
                     ELTSNPLVRKLSFTGSTEIGRQLMEQCAKDIKKVSLELGGNAPFIVFDDADLDKAVEG
                     ALASKFRNAGQTCVCANRLYVQDGVYDRFAEKLQQAVSKLHIGDGLEKGVTIGPLIDE
                     KAVAKVEEHIADALEKGARVVCGGKAHERGGNFFQPTILVDVPANAKVSKEETFGPLA
                     PLFRFKDEADVIAQANDTEFGLAAYFYARDLSRVFRVGEALEYGIVGINTGIISNEVA
                     PFGGIKASGLGREGSKYGIEDYLEIKYMCIGL"
     gene            3837..5117
                     /gene="gabT"
                     /locus_tag="J1Y83_003876"
     CDS             3837..5117
                     /gene="gabT"
                     /locus_tag="J1Y83_003876"
                     /EC_number="2.6.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000097653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-aminobutyrate--2-oxoglutarate transaminase"
                     /protein_id="HBA7631979.1"
                     /translation="MSSNKELMQRRSQAIPRGVGQIHPIFADRAENCRVWDVEGREYL
                     DFAGGIAVLNTGHLHPKVVAAVEAQLKKLSHTCFQVLAYEPYLELCEIMNQKVPGDFA
                     KKTLLVTTGSEAVENAVKIARAATKRSGTIAFSGAYHGRTHYTLALTGKVNPYSAGMG
                     LMPGHVYRALYPCPLHGISEDDAIASIHRIFKNDAAPEDIAAIVIEPVQGEGGFYAAT
                     PAFMQRLRALCDEHGIMLIADEVQSGAGRTGTLFAMEQMGVAPDLTTFAKSIAGGFPL
                     AGVTGRAEVMDAVAPGGLGGTYAGNPIACVAALEVLKVFEQENLLQKANVLGQKLKDG
                     LLAIAEKHTEIGDVRGLGAMIAIELFEDGDHSKPDAKLTAEIVARARDKGLILLSCGP
                     YYNVLRILVPLTIEDAQIRQGLEIISQCFAEAKL"
     gene            5354..6754
                     /gene="gabP"
                     /locus_tag="J1Y83_003877"
     CDS             5354..6754
                     /gene="gabP"
                     /locus_tag="J1Y83_003877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GABA permease"
                     /protein_id="HBA7631980.1"
                     /translation="MGQSSQPHELGGGLKSRHVTMLSIAGVIGASLFVGSSVAIAEAG
                     PAVLLAYLFAGLLVVMIMRMLAEMAVATPDTGSFSTYADKAIGRWAGYTIGWLYWWFW
                     VLVIPLEANIAAMILHSWVPGIPIWLFSLVITLALTGSNLLSVKNYGEFEFWLALCKV
                     IAILAFIFLGAVAISGFYPYADVSGISRLWDSGGFMPNGFGAVLSAMLITMFSFMGAE
                     IVTIAAAESDTPEKHIVRATNSVIWRISIFYLCSIFVVVALIPWNMPGLKAVGSYRSV
                     LELLNIPHAKLIMDCVILLSVTSCLNSALYTASRMLYSLSRRGDAPAVMGKINRSKTP
                     YVAVLLSTGAAFLTVVVNYYAPAKVFKFLIDSSGAIALLVYLVIAVSQLRMRKILRAE
                     GSEIRLRMWLYPWLTWLVIGFITFVLVVMLFRPAQQLEVISTGLLAIGIICTVPIMAR
                     WKKLIMWQKTPIHNTR"
     gene            6775..7437
                     /gene="csiR"
                     /locus_tag="J1Y83_003878"
     CDS             6775..7437
                     /gene="csiR"
                     /locus_tag="J1Y83_003878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311552.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CsiR"
                     /protein_id="HBA7631981.1"
                     /translation="MTITSLDGYRWLKNDIIRGNFQPDEKLRMSLLTSRYALGVGPLR
                     EALSQLVAERLVTVVNQKGYRVASMSEQELLDIFDARANMEAMLVSLAIARGGDEWEA
                     DVLAKAHLLSKLEACDASEKMLDEWDLRHQAFHTAIVAGCGSHYLLQMRERLFDLAAR
                     YRFIWLRRTVLSVEMLEDKHDQHQTLTAAVLARDTARASELMRQHLLTPIPIIQQAMA
                     GN"
     gene            complement(7438..7887)
                     /gene="kbp"
                     /locus_tag="J1Y83_003879"
     CDS             complement(7438..7887)
                     /gene="kbp"
                     /locus_tag="J1Y83_003879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium binding protein Kbp"
                     /protein_id="HBA7631982.1"
                     /translation="MGLFNFVKDAGEKLWDAVTGQHDKDDQAKKVQEHLNKTGIPDAD
                     KVNIQIADGKATVTGDGLSQEAKEKILVAVGNISGIASVDDQVKTATPAAASQFYTVK
                     SGDTLSAISKQVYGNANLYNKIFEANKPMLKSPDKIYPGQVLRIPEE"
     gene            complement(7971..>8102)
                     /locus_tag="J1Y83_003880"
     CDS             complement(7971..>8102)
                     /locus_tag="J1Y83_003880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqaE/Pmp3 family membrane protein"
                     /protein_id="HBA7631983.1"
                     /translation="IILPPLGVLLGKGFGWAFIINILLTLLGYIPGLIHAFWVQTRD"
BASE COUNT         1804 a         2141 c         2303 g         1854 t
ORIGIN      
        1 ttttcagaaa tgtagatatt tttagattat ggctacgaaa tgagcatcgc catgtcaccc
       61 tacatctcat aagaggatcg cttctgatga atgcactgac cgccgtacaa aataacgctg
      121 tcgattcagg ccaggactat agcggattca ctctcatccc gtcggcgcaa tccccgcgtc
      181 tgctggaact caccttcacc gaacagacga ccaaacagtt tctcgaacag gttgccgaat
      241 ggcccgtaca ggcgctggag tacaaatctt ttctgcgttt tcgggtaggc aaaattctcg
      301 acgatctgtg tgcgaatcag ctgcaaccgc tgctgttgaa gaccctgtta aaccgcgctg
      361 aaggtgcgct gttgatcaat gcggtgggta ttgatgatgt cgcgcaggcg gatgagatgg
      421 tgaagctggc gacggcggtg gcgcatctga ttggtcgctc aaatttcgat gcgatgagcg
      481 gtcagtatta cgcgcgattc gtggtgaaaa atgtcgataa ctcagacagc tatctgcgtc
      541 agccgcaccg cgtaatggag ctacacaacg acggcaccta cgtcgaagag atcaccgact
      601 atgtgctgat gatgaaaatc gacgagcaga acatgcaggg tggaaattcg ttgctgctgc
      661 atctcgatga ctgggaacat ctggaccact atttccgcca cccgctggcg cgtcgcccga
      721 tgcgctttgc cgcgccgccg agcaaaaacg tcagcaaaga tgttttccat ccggtgttcg
      781 acgttgatca acagggtcgc ccggtgatgc gttatatcga ccagttcgtc cagccaaaag
      841 acttcgaaga aggcgtgtgg ctgagcgagc tttccgatgc cattgaaacc agcaaaggca
      901 ttctttctgt gcccgttccc gttggtaaat tcctgttgat taacaatcta ttctggctgc
      961 acggtcgcga ccgctttact ccgcacccgg atctgcgccg tgaactgatg cgtcagcgtg
     1021 gctatttcgc ttacgccact aaccactacc agacgcatca gtaagcgcaa aggaattgag
     1081 cggatgtatg attttgtgat tattggcggc ggcatcatcg gcatgtcgac cgccatgcaa
     1141 ctgattgatg tttatccgga tgcccgcatt gcgttgctgg aaaaagagtc cggcccggcc
     1201 tttcatcaga cgggccacaa cagcggcgtg atccatgccg gggtctatta cacgcccggt
     1261 agcctgaagg cacagttttg cctggcggga aaccgcgcca ccaaagcctt ttgcgatcaa
     1321 aacggcattc gctatgacaa ctgcggcaag atgctggtcg ccacctctga actcgaaatg
     1381 gaacggatgc gcgcgttgtg ggaacgcacg gcggcgaacg gtatcgagcg cgagtggtta
     1441 aaagccgagg aactgcgcga gcgcgaaccg aatatcaccg ggctcggcgg catttttgtg
     1501 ccgtccagcg gcattgtcag ctatcgcgaa gtgacggcgg cgatggcaaa aatcttccag
     1561 gccagaggcg gcgagattat ttataacgcc gaagtcagcg ccctcagtga gcataaaaac
     1621 ggcgtggtga tacgtacccg tcagggcggt gaatatgaag catcgacgct gattagctgt
     1681 tccgggctga tggctgaccg gctggtgaaa atgctcggcc tcgaaccggg ctttattatc
     1741 tgcccgttcc gtggcgagta tttccgcctt gcgccggagc ataaccagat tgttaaccac
     1801 ctgatttacc ccattcccga ccccgcgatg ccatttttgg gcgtgcatct cacccgcatg
     1861 atcgatggca gcgtgaccgt cgggccaaac gcggtgctgg cttttaaacg cgaaggctat
     1921 cgcaagcgcg atttctcgtt tagtgacacg ctggagattt taggctcgtc ggggattcgc
     1981 cgggtgctgc aaaaccatct acgctcagga ttgggcgaga tgaaaaactc gctgtgcaaa
     2041 agcggctatc tgcggctggt gcaaaagtat tgtccccggc tttcgttaag cgatctccag
     2101 ccctggcccg ccggtgtgcg ggcgcaggcg gtatcgccgg acggcaagct gattgacgat
     2161 tttctgtttg tcaccacccc gcgcacgatc cacacctgca atgcgccctc cccggcagcg
     2221 acatcagcaa ttcctattgg tgcgcatatt gtcagcaagg tacaaacgct gttggcaagc
     2281 cagagtaacc ccggacgcac gctgcgagcg gcacgtagtg tggatgcctt acacgccgca
     2341 tttaatcaat aacctttgaa aacaggacgt aacaatgaaa cttaacgaca gtaacttatt
     2401 ccgccagaag gcgttgatta atggggaatg gctggacgcc aacaatggcg aggtcatcga
     2461 cgtcaccaat ccggcgaacg gcgacaagct gggtagcgtg ccgaaaatgg gcgctgatga
     2521 aacccgcgcc gctatcgacg ccgccaaccg cgccctgccc gcctggcgtg cgctcaccgc
     2581 caaagaacgc gccaacattc tgcgcaactg gttcaatttg atgatggagc atcaggacga
     2641 tttagcgcgc ctgatgaccc tcgaacaggg caaaccactg gcggaagcga aaggcgaaat
     2701 cagctacgca gcctctttta ttgagtggtt tgccgaagaa ggcaaacgta tttatggcga
     2761 taccattccc ggtcatcagg ccgacaaacg cctgattgtt atcaaacagc cgattggcgt
     2821 taccgccgcc atcacgccgt ggaacttccc ggcggcgatg attacccgta aagccggtcc
     2881 ggcgctggcg gcaggctgca cgatggtgct gaaacctgcc agtcagacgc cgttctctgc
     2941 gctggcgctg gcggaactgg cgattcgcgc aggcgttccg gctggcgtat ttaacgtggt
     3001 caccggttcg gcgggcgcgg tcggtaacga actgaccagc aatccgctgg tgcgcaagct
     3061 gtcgtttacc ggctcgaccg aaattggccg ccagttaatg gaacagtgcg cgaaagacat
     3121 caagaaagtg tcgctggagc tgggcggcaa cgcgccgttt atcgtctttg acgatgccga
     3181 tctcgataaa gccgtggaag gcgcgctggc ttctaaattc cgcaacgccg ggcaaacctg
     3241 cgtctgtgcc aaccgtttat atgtgcagga cggcgtgtat gaccgctttg ccgaaaaatt
     3301 acagcaggcg gtgagcaaac tgcacatcgg cgacgggctg gagaaaggcg tcaccatcgg
     3361 gccgctgatt gatgaaaaag cggtagcaaa agtggaagag catattgccg atgcgctgga
     3421 gaaaggtgcg cgcgtggttt gcggcggtaa agcacatgaa cgcggcggca acttcttcca
     3481 gccgaccatt ctggtggacg ttccggccaa cgccaaagtg tcgaaagaag agacgttcgg
     3541 cccccttgcc ccgctattcc gctttaaaga tgaagccgat gtgattgcgc aggccaatga
     3601 caccgagttt ggccttgccg cctatttcta cgcccgtgat ttaagccgcg tcttccgcgt
     3661 gggcgaagcg ctggagtacg gcatcgtcgg catcaatacc ggcattattt ccaatgaagt
     3721 ggccccgttc ggcggcatta aagcctcggg tctgggtcgt gaaggttcga agtatggcat
     3781 cgaagattac ttagaaatca aatatatgtg catcggtctt taactggaga acgcgaatga
     3841 gcagcaataa agagttaatg cagcgccgca gtcaggcgat tccccgtggc gttgggcaaa
     3901 ttcacccgat tttcgctgac cgcgcggaaa actgccgggt gtgggacgtt gaaggccgtg
     3961 agtatcttga tttcgcgggc gggattgcgg tgctcaatac cgggcacctg catccgaagg
     4021 tggtggccgc ggtggaagcg cagttgaaaa aactgtcgca cacctgcttc caggtgctgg
     4081 cttacgagcc gtatctggag ctgtgcgaga ttatgaatca gaaggtgccg ggcgatttcg
     4141 ccaagaaaac gctgctggtt acgaccggtt ccgaagcggt ggaaaacgcg gtgaaaatcg
     4201 cccgcgccgc caccaaacgt agcggtacta tcgcctttag cggtgcgtat cacgggcgca
     4261 cgcactacac gctggcgctg accggtaagg tgaacccata ctctgcgggc atgggactca
     4321 tgccgggtca tgtttatcgc gcgctttatc cttgcccgct gcacggcata agcgaggatg
     4381 acgctatcgc cagcatccac cggatcttca aaaatgacgc cgcgccggaa gatatcgctg
     4441 ccatcgtgat tgagcccgtt cagggcgaag gcggtttcta cgccgcgacg ccagccttta
     4501 tgcagcgttt acgcgcgctg tgtgacgagc acgggatcat gctgattgcc gatgaagtgc
     4561 agagcggcgc agggcgtacc ggcacgctgt ttgcgatgga gcaaatgggc gtggcaccag
     4621 atctcaccac ctttgcgaaa tcgatcgcag gcggcttccc gctggcgggc gtcaccggtc
     4681 gcgccgaggt gatggacgcc gtcgcaccag gcgggctggg cggcacctat gcgggtaacc
     4741 cgattgcctg cgtggcggcg ctggaagtgt tgaaggtgtt cgagcaggaa aatctgctgc
     4801 aaaaagccaa cgttctgggg cagaagttga aagatggatt gctggcgatc gccgaaaaac
     4861 acaccgagat cggcgacgta cgcggcctgg gagcgatgat tgccatcgag ctgtttgaag
     4921 atggcgatca cagcaaaccg gacgcaaaac tcaccgccga gatcgtggca cgcgcccgcg
     4981 ataagggtct gattcttctc tcctgcggcc cgtattacaa cgtgctgcgc atccttgtac
     5041 cgctcaccat tgaagacgct cagatccgtc agggtctgga gatcatcagc cagtgttttg
     5101 ctgaggcgaa gctgtagcac cgcaccatgc cggaggcgac gctttgcggc ttgtccggcc
     5161 tacgagaatc caggtcggat aaggcgttta cgccgcatcc gacaatctgt acgtgaacag
     5221 gaataaatct atgtcggccg gataaggcgg agccgctctc cggcaaaaag aaccaataac
     5281 aattaaacgc gtgacccgac gcgggaaatg tcggggcgct ctcccaagtg acacactttc
     5341 gagaggattc aggatggggc aatcatcgca accacatgag ttaggcggcg ggctgaagtc
     5401 acgccacgtc accatgttgt ctattgccgg tgttatcggc gcaagtctgt ttgtcggttc
     5461 cagcgtcgcc atcgccgaag cgggcccggc ggtattactg gcctatctgt tcgccggatt
     5521 actggtggtt atgattatgc ggatgttggc ggaaatggcg gttgccacgc ccgataccgg
     5581 ttcgttttcc acctatgccg ataaagccat tggacgttgg gcgggctata ccatcggctg
     5641 gttgtactgg tggttttggg tactggttat cccactggaa gccaacatcg ccgccatgat
     5701 cctgcactcg tgggttccag gcattcccat ctggttattt tccctcgtca ttaccctcgc
     5761 cttaaccggc agtaatttat taagcgttaa aaactacggc gaatttgagt tctggctggc
     5821 gctgtgtaaa gtcatcgcta tcctggcctt tattttcctt ggtgcagtcg caattagcgg
     5881 tttttaccct tatgccgacg tgagcggtat ttcaagattg tgggatagcg gcggctttat
     5941 gcccaacggc ttcggtgcgg tattaagcgc gatgttgatc accatgttct cgtttatggg
     6001 cgcagaaatt gtcaccattg ccgccgcgga atccgacacg ccggaaaaac atattgtccg
     6061 cgccaccaac tcggttatct ggcgtatttc tatcttctat ttatgctcta tttttgtcgt
     6121 cgtggcatta ataccgtgga atatgccggg gctgaaagcc gttggatctt atcgctcggt
     6181 tctggagttg ctcaatattc cccatgcgaa attaatcatg gactgcgtga tattactttc
     6241 cgtaaccagt tgcctgaact cggcgctgta taccgcttcc agaatgctct actccttaag
     6301 ccgtcgcggt gatgcgcccg cggtaatggg taaaatcaac cgcagtaaaa ccccgtatgt
     6361 ggcggtatta ctctccaccg gcgcggcatt tttaacggtg gtggtgaatt attacgcgcc
     6421 tgcgaaagtg tttaaatttc tgatcgacag ctccggtgct atcgccctgc tggtttattt
     6481 agtcatcgcc gtttcacaat tgcggatgcg caaaattctg cgagcagaag gaagcgaaat
     6541 tcgcctgcgg atgtggcttt atccgtggct cacctggctg gtcatcggct ttattacctt
     6601 tgtgttggta gtgatgctat tccgcccggc gcaacagtta gaagtgatct ccaccggctt
     6661 attagcgatt gggattatct gtaccgtgcc aattatggct cgctggaaaa agctgataat
     6721 gtggcaaaaa acacccattc ataatacgcg ctgataatga tcaggagtca caccatgacc
     6781 attacctctc tggatggcta tcgctggctg aagaacgata ttattcgcgg caattttcag
     6841 ccggatgaaa aattacggat gagtttgctg acatcgcgtt atgcacttgg cgtcggaccg
     6901 ttgcgggaag cgctttcgca actggtggca gaacgactgg tcacggtggt gaatcaaaaa
     6961 gggtatcggg tggcgtctat gtcagagcag gagctgctcg atattttcga cgcccgcgcc
     7021 aatatggaag cgatgttagt gagtctggcg attgcccgcg gtggcgatga gtgggaggca
     7081 gacgttctcg caaaagcgca tctgctcagt aagctggagg cctgtgacgc cagtgagaaa
     7141 atgcttgatg agtgggatct tcgtcatcag gcgtttcata ctgcaattgt ggcgggctgt
     7201 ggttctcact atttgctgca aatgcgtgaa cggttgtttg atctggcggc gcgctatcga
     7261 tttatctggc tgcggcgaac agtgctttcg gtggaaatgc tggaggataa acacgatcag
     7321 caccagaccc tgactgcggc ggtactggcg cgagataccg cgcgcgccag tgagttaatg
     7381 cgccagcatt tactgacgcc aattcccatt atccagcagg cgatggctgg caattaacta
     7441 ctcttcagga atacgcaaca cttgccccgg gtaaatttta tccgggcttt ttagcatcgg
     7501 tttattcgct tcgaagattt tattgtacag attagcgtta ccgtagacct gtttggaaat
     7561 ggcactcaga gtgtcgccag acttaacggt ataaaactgg ctggcagcgg ctggtgtcgc
     7621 cgttttcacc tgatcatcga cactggcaat accggaaata ttccccaccg caacaaggat
     7681 tttctccttc gcctcctgac ttaagccgtc acccgtgacc gtcgctttgc cgtcggcgat
     7741 ttgaatgttc actttatcgg catcaggtat accggttttg ttcagatgct cctgcacctt
     7801 cttcgcctga tcgtctttat cgtgctgacc tgtaaccgcg tcccagagtt tttctccggc
     7861 atctttcaca aaattgaaca gacccatagc tacctcattc gttaacggaa acactcagaa
     7921 agtgtagcag aggaacgcgg cgcgttaacg ccgcgggggt gagcacaggt ttaatcgcgg
     7981 gtttgcaccc agaacgcgtg aatcaaaccg gggatatacc caagcagtgt cagcagaata
     8041 ttaataatga acgcccaacc gaacccttta ccgagcagca cgccgagcgg cggcagaatg
     8101 at
//
LOCUS       DADSYM010000176         8031 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2415
            SAMN08039579-rid11723173.denovo.178, whole genome shotgun sequence.
ACCESSION   DADSYM010000176 DADSYM010000000
VERSION     DADSYM010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039579
            Sequence Read Archive: SRR6362533
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8031)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8031)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980162.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 03:39:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,484
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 4,347
            CDSs (with protein)               :: 4,347
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 43 of 85
            Pseudo Genes (incomplete)         :: 37 of 85
            Pseudo Genes (internal stop)      :: 19 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8031
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039579-rid11723173.denovo.178"
                     /strain="LGE2415"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8302 N 95.9628 W"
                     /collection_date="2015-08-24"
     gene            32..1402
                     /locus_tag="J1Y83_003881"
     CDS             32..1402
                     /locus_tag="J1Y83_003881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706814.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anion permease"
                     /protein_id="HBA7631984.1"
                     /translation="MLAPAGLSELAWVLFGIYLAAIVGLVIKPFPEPVVLLIAVAASM
                     VVVGNLSGGEFKTTAVLSGYSSGTTWLVFSAFTLSAAFVTTGLGKRIAYLLIGKIGST
                     TLGLGYVTVFLDLVLAPATPSNTARAGGIVLPIINSVAVALGSEPEKSPRRVGHYLMM
                     SIYMVTKTTSYMFFTAMAGNILALKMINDILHLQISWGGWALAAGLPGIIMLLVTPLV
                     IYTMYPPEIKKVDNKTIAKAGLAELGPMKIREKMLLGVFVLALLGWIFSKSLGVDEST
                     VAIVVMATMLLLGIVTWEDVVKNKGGWNTLIWYGGIIGLSSLLSKVKFFEWLAEVFKN
                     NLAFDGHGNVAFFVIIFLSIIVRYFFASGSAYIVAMLPVFAMLANVSGAPLMLTALAL
                     LFSNSYGGMVTHYGGAAGPVIFGVGYNDIKSWWLVGAVLTILTFLVHITLGVWWWNML
                     IGWNML"
     gene            1585..3846
                     /locus_tag="J1Y83_003882"
     CDS             1585..3846
                     /locus_tag="J1Y83_003882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415292.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydratase"
                     /protein_id="HBA7631985.1"
                     /translation="MIKLSEKGVFLASNNEIIAEEHFTGEIKKEEAKKGTIAWSILSS
                     HNTSGNMDKLKIKFDSLASHDITFVGIVQTAKASGMERFPLPYVLTNCHNSLCAVGGT
                     INGDDHVFGLSAAQRYGGIFVPPHIAVIHQYMREMMAGGGKMILGSDSHTRYGALGTM
                     AVGEGGGELVKQLLNDTWDIDYPGVVAVHLTGKPAPYVGPQDVALAIIGAVFKNGYVK
                     NKVMEFVGPGVAALSTDFRNSVDVMTTETTCLSSVWQTDEEVHNWLALHGRGQDYCQL
                     NPQPMAYYDGCISVDLSAIKPMIALPFHPSNVYEIDTLNQNLTDILREIEIESERVAH
                     GKAKLSLLDKVENGRLKVQQGIIAGCSGGNYENVIAAANALRGQSCGNDTFSLAVYPS
                     SQPVFMDLAKKGVVADLIGAGAIIRTASCGPCFGAGDTPINNGLSIRHTTRNFPNREG
                     SKPANGQMSAVALMDARSIAATAANGGYLTSASELDCWDNVPEYAFDVTPYKNRVYQG
                     FVKGATQQPLIYGPNIKDWPELGALTDNIVLKVCSKILDEVTTTDELIPSGETSSYRS
                     NPIGLAEFTLSRRDPGYVGRSKATAELENQRLAGNVSEMADVFARIKQIAGQEHIDPL
                     QTEIGSMVYAVKPGDGSAREQAASCQRVIGGLANIAEEYATKRYRSNVINWGMLPLQM
                     AEAPTFEVGDYIYIPGIKAALDNPGTTFKGYVIHEDAPVTEITLYMESLTAEEREIIK
                     AGSLINFNKNRQM"
     gene            complement(3986..5269)
                     /locus_tag="J1Y83_003883"
     CDS             complement(3986..5269)
                     /locus_tag="J1Y83_003883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative acyl-CoA thioester hydrolase"
                     /protein_id="HBA7631986.1"
                     /translation="MNTFSVSRLALALAFGVTLTACSSTPPDQRPSDQTAPGTSSRPI
                     LSAKEAQNFDAQHYFASLTPGAAAWNPSPITLPAQPDFVVGPAGTQGVTHTTIQAAVD
                     AAIIKRTNKRQYIAVMPGEYQGTVYVPAAPGGITLYGTGEKPIDVKIGLSLDGGMSPA
                     DWRHDVNPRGKYMPGKPAWYMYDSCQSKRSDSIGVLCSAVFWSQNNGLQLQNLTIENT
                     LGDSVDAGNHPAVALRTDGDKVQINNVNILGRQNTFFVTNSGVQNRLETNRQPRTLVT
                     NSYIEGDVDIVSGRGAVVFDNTEFRVVNSRTQQEAYVFAPATLSNIYYGFLAVNSRFN
                     ASGDGVAQLGRSLDVDANTNGQVVIRDSAINEGFNTAKPWADAVISNRPFAGNTGSVD
                     DNDEVQRNLNDTNYNRMWEYNNRGVGSKVVAEAKK"
     gene            complement(5404..6474)
                     /locus_tag="J1Y83_003884"
     CDS             complement(5404..6474)
                     /locus_tag="J1Y83_003884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HBA7631987.1"
                     /translation="MGRRRSHERRDLPPNLYIRNNGYYCYRDPRTGKEFGLGRDRRIA
                     ITEAIQANIELFSGHKHKPLTARINSDNSVTLHSWLDRYEKILASRGIKQKTLINYMS
                     KIKAIRRGLPDAPLEDITTKEIAAMLNGYIDEGKAASAKLIRSTLSDAFREAIAEGHI
                     TTNPVAATRAAKSEVRRSRLTADEYLKIYQAAESSPCWLRLAMELAVVTGQRVGDLCE
                     MKWSDIVDGYLYVEQSKTGVKIAIPTTLHVDALGISMKETLDKCKEILGGETIIASTR
                     REPLSSGTVSRYFMRARKASGLSFEGDPPTFHELRSLSARLYEKQISDKFAQHLLGHK
                     SDTMASQYRDDRGREWDKIEIK"
     gene            complement(6452..6670)
                     /locus_tag="J1Y83_003885"
     CDS             complement(6452..6670)
                     /locus_tag="J1Y83_003885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001325331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HBA7631988.1"
                     /translation="MYLTLQEWNARQRRPRSLETVRRWVRECRIFPPPVKDGREYLFH
                     ESAVKVDLNRPVTGSLLKRIRNGKKAKS"
     gene            complement(6710..6877)
                     /locus_tag="J1Y83_003886"
     CDS             complement(6710..6877)
                     /locus_tag="J1Y83_003886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA7631989.1"
                     /translation="MHFRVTGEWNGEPFNRVIEAENINDCYDHWMIWAQIAHADITNI
                     RIEELKEHQAA"
     gene            7120..7722
                     /locus_tag="J1Y83_003887"
     CDS             7120..7722
                     /locus_tag="J1Y83_003887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA7631990.1"
                     /translation="MSYFLRKKWMVNLSGAGKILWTLNMKKDSYPYLICMTVSGLIFI
                     FLFFWWRADIYRVTFLNQSISHYYILFSMGIAFLLSLFWVKKGIVKQSGWKSLSAYLK
                     VYAGMCIFAGFFLIIPLTTLTYFLPGETSSYVAPYRYTSGSSKSCSGAEVDDPDLHEN
                     IRICYPYGNYEYDNIIYVEKKINTLGAVVTYAQTARDDTE"
     gene            complement(7933..>8031)
                     /locus_tag="J1Y83_003888"
     CDS             complement(7933..>8031)
                     /locus_tag="J1Y83_003888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA family transcriptional regulator"
                     /protein_id="HBA7631991.1"
                     /translation="IDERRRLAVQGCRTCASCQEDLELISKQRGSK"
BASE COUNT         1900 a         1894 c         1986 g         2251 t
ORIGIN      
        1 gatattagcg atcccatgta ttattggctt tatgctagct ccggcaggat taagcgaact
       61 ggcgtgggtg ctttttggta tttacctggc ggccattgtg gggctggtta tcaagccttt
      121 cccggaacct gtcgtactgt taattgccgt cgccgcatcg atggtagtgg tgggtaactt
      181 atcaggtggg gaatttaaaa ccaccgccgt attaagcggt tactcttccg gtaccacctg
      241 gctggtgttt tctgcgttta ctttaagcgc cgcgtttgta accacaggct taggtaaacg
      301 tattgcctat ctgctgattg gtaaaattgg tagcactacc ctgggtctgg gttacgttac
      361 ggtatttctc gatctggtac tggctccggc aacaccgtct aacaccgcgc gtgcgggcgg
      421 catcgtgtta ccgatcatca acagcgtggc agtggctttg ggatcagaac cggaaaaaag
      481 tccgcgtcgt gttggacatt acctgatgat gtccatttac atggtcacta aaaccaccag
      541 ctatatgttc tttaccgcaa tggcggggaa cattctggcg ctgaaaatga tcaacgacat
      601 tctgcacctg caaattagct ggggtggatg ggcgctagcc gccggattgc ctggcatcat
      661 tatgctgctg gtcaccccgc tggtgattta caccatgtat ccaccagaaa ttaagaaggt
      721 ggataacaaa accatcgcta aagcgggcct tgccgaactg ggaccgatga aaatccgcga
      781 aaaaatgctg ctcggtgtct ttgtgctggc gctgctgggc tggattttca gtaagtctct
      841 gggggttgat gaatccaccg tggcaatcgt tgttatggca actatgctgc tgctgggtat
      901 cgttacctgg gaagacgtgg ttaaaaataa aggcggctgg aataccttaa tctggtacgg
      961 cggtattatc ggcttaagct ccttattatc gaaagttaaa ttcttcgaat ggttagctga
     1021 agtctttaaa aataacctgg catttgatgg tcacggtaac gttgctttct tcgttattat
     1081 tttcctcagc atcatcgtgc gttatttctt cgcttctggt agtgcctata tcgttgccat
     1141 gttaccggta tttgccatgc tggcgaatgt ctccggcgca ccgttaatgt taaccgcgct
     1201 ggcactgttg ttctctaact cctatggcgg catggttact cactatggcg gtgcggcagg
     1261 tccggtcatc tttggcgtgg gttacaacga tattaaatcc tggtggttgg tcggtgcggt
     1321 actgacgata ttaaccttcc tggtgcatat cacccttggc gtgtggtggt ggaatatgct
     1381 gatcggctgg aacatgctgt aaatatactc gtcatacttc aagttgcatg tgctgcgtct
     1441 gcgttcgctc accccagtca cttacttatg taagctcctg gggattcact cgcttgtcgc
     1501 cttcctgcaa ctcgaattat ttagagtata tccatttatt atctttctgc gcacttcacg
     1561 gtgcgcagat atctggagca tttgatgatc aagttatctg aaaaaggcgt gtttctcgcc
     1621 agcaataacg aaataattgc cgaagaacat ttcaccggcg aaataaaaaa agaagaagca
     1681 aaaaaaggca ctattgcctg gtctattctc tcttctcata atacgtccgg aaatatggat
     1741 aaacttaaaa ttaagtttga ttcattagcc tctcacgata ttacctttgt tggtattgta
     1801 cagaccgcta aagcgtccgg tatggaacgt ttcccgctgc cgtatgtgct gaccaactgc
     1861 cataactcac tctgcgccgt cggcggcacc attaacggtg atgaccatgt ttttggttta
     1921 tcggcggcgc agcgttatgg cggtattttt gtgccaccgc atattgcggt catccatcaa
     1981 tatatgcgtg aaatgatggc aggcggcggc aaaatgatcc tcgggtcaga cagccatacc
     2041 cgttacggtg cgttagggac aatggcggtt ggcgagggcg gcggcgagtt ggtaaaacag
     2101 ctgcttaatg acacctggga tatcgactat ccgggcgtgg ttgcggtgca tctgaccgga
     2161 aaacctgcgc cgtatgtggg gccgcaggat gtggcgctgg ctatcatcgg tgctgtgttc
     2221 aaaaacggtt acgtcaaaaa caaagtgatg gagttcgtag gtcccggtgt tgcagcgctc
     2281 tctaccgatt tccgtaacag cgttgacgtg atgaccactg aaacgacctg tttaagttct
     2341 gtctggcaaa ccgatgaaga agtccataac tggctggcgc tgcacggtcg cggccaggat
     2401 tactgccaac ttaaccctca accaatggcg tactacgatg gctgcatcag cgttgattta
     2461 agcgccatca aaccaatgat tgcgctgcca ttccacccga gcaacgtgta tgaaatcgac
     2521 acgctgaacc agaacctgac cgacattctg cgtgagattg aaattgagtc cgaacgcgtg
     2581 gcgcacggta aagccaaact ctcgctgctg gataaagtgg aaaatggtcg cctgaaagtg
     2641 cagcagggga ttatcgcggg ctgttctggc ggtaactatg aaaacgtcat cgcggcggcg
     2701 aatgcgctgc gtggtcaatc ctgcggcaac gataccttct cgttggcggt ttacccgtca
     2761 tcacagccgg tgtttatgga tctcgccaaa aaaggtgtgg tcgcagattt gattggcgca
     2821 ggcgcaatca tcagaaccgc gtcctgcggc ccatgctttg gcgcgggcga tacgccaatc
     2881 aacaacggtt tgagtattcg ccacaccacg cgtaacttcc cgaaccgcga aggctctaag
     2941 ccagctaatg ggcagatgtc agcggtggcg ttgatggacg ctcgttctat cgctgcgact
     3001 gcggcaaacg gtggctattt aacctctgcc agcgaactcg attgctggga caacgtgccg
     3061 gagtacgcct tcgatgtaac gccgtataaa aaccgtgttt atcagggctt tgtgaaaggg
     3121 gcgacccagc aaccgctgat ttacggaccg aacattaaag actggccgga attgggtgcg
     3181 ctgactgaca atatcgtcct caaagtgtgc tcgaagatcc tcgacgaagt gaccaccacc
     3241 gacgaactga ttccttccgg tgaaacctct tcttatcgtt caaatccgat tggtctggcg
     3301 gagtttaccc tgtcacgccg cgatccaggt tatgttggca gaagtaaagc gactgctgag
     3361 ctggaaaatc agcgtctggc ggggaatgtc agcgagatgg cagatgtgtt tgcgcgcatt
     3421 aagcagattg ctggtcagga gcatattgat ccgctgcaaa ctgaaattgg cagtatggtc
     3481 tatgcggtga aaccaggcga tggttctgcg cgtgaacagg cggcgagttg tcagcgtgtg
     3541 attggcggtc tggcgaatat tgccgaggag tacgcgacta aacgctatcg ttctaacgtc
     3601 atcaactggg ggatgttacc gctgcagatg gcggaagcgc caacctttga agtgggggat
     3661 tacatttaca tccctggcat taaagcggcg ctggataatc ctggaacgac gtttaaaggt
     3721 tatgtgatcc atgaagatgc accggtaacg gaaatcacgc tgtatatgga aagtctgact
     3781 gccgaagaac gcgagattat caaggcgggt agtttgatta acttcaataa aaaccgtcag
     3841 atgtaaaaag cgccatgtga atgtaggtcg catccgcagt tattgtcgga tgcgatgccg
     3901 acgcatctta tccgaccgac aaatcgtatc gaactgtagg ccggataagg cgtttacgcc
     3961 gcatccggca catagttaac agctcctact tcttcgcctc tgcaaccact ttgctaccca
     4021 cgccgcggtt attgtattcc cacatgcggt tgtagttagt gtcattcaga ttgcgctgta
     4081 cttcgtcgtt atcatcaacg ctgccggtgt tacccgcaaa cggacgatta gagatcaccg
     4141 catcagccca cggtttggct gtgttaaaac cttcgttgat ggcgctatca cggatcacta
     4201 cctgaccgtt ggtattggca tcaacatcca gcgagcggcc cagttgcgcc acgccatcac
     4261 cggaagcatt gaaacggctg tttacggcga ggaaaccgta gtaaatgttg gacagcgtag
     4321 ccggtgcaaa cacatacgct tcttgctggg tacgggagtt caccacgcgg aattcggtgt
     4381 tatcgaacac cactgcgccg cgaccagaaa cgatatccac atccccttca atatagctgt
     4441 tggtcaccag cgtacgcggc tggcgattcg tttccagacg gttctgcaca ccgctgttgg
     4501 tgacaaagaa ggtgttctga cgaccgagaa tgttgacgtt attgatctgc actttgtcgc
     4561 catcagtacg cagtgccacc gccggatggt tacccgcatc tacgctatcg cccagcgtgt
     4621 tttcgatggt caggttttgc agttgcaggc cattgttttg tgaccagaag accgcagagc
     4681 agagaacacc gatactgtcg ctgcgtttac tctggcagct atcgtacata taccacgccg
     4741 gtttacctgg catatatttg ccgcgcgggt tgacgtcgtg acgccagtcg gcagggctca
     4801 tgccaccatc aagggaaagc ccaatcttca catcaatcgg tttttcacct gtaccgtaca
     4861 gagtaattcc acccggagcg gcagggacat acaccgttcc ctgatactca ccaggcatca
     4921 cggcaatata ctggcgcttg ttggtacgct tgataattgc cgcatctacc gccgcctgaa
     4981 tcgtggtatg cgttacacct tgagtacccg ccgggccgac aacaaagtca ggttgcgcag
     5041 gcagggtaat cggggaagga ttccacgctg ccgcacctgg tgtcagggat gcaaaatagt
     5101 gttgagcatc gaaattctgc gcttcttttg ccgacagaat cgggcgagaa gaggtaccag
     5161 gcgcggtttg atcagaagga cgttgatcgg gcggtgttga gctacaggcg gtcagcgtca
     5221 cgccaaaagc caatgccagc gccagacggg aaactgaaaa tgtgttcaca ggttgctccg
     5281 ggctatgaaa tagaaaaatg aatccgttga agcctgcttt tttatactaa gttggcatta
     5341 taaaaaaaca ttgcttatca atttgttgca acgaacaggt cactatcagt caaaataaaa
     5401 tcattatttg atttcaattt tgtcccactc cctgcctctg tcatcacgat actgtgatgc
     5461 catggtgtcc gacttatgcc cgagaagatg ttgagcaaac ttatcgctta tctgcttctc
     5521 atagagtctt gcagacaaac tgcgcaactc gtgaaaggta ggcggatccc cttcgaagga
     5581 aagacctgat gcttttcgtg cgcgcataaa ataccttgat actgtgccgg atgaaagcgg
     5641 ttcacgacga gtagatgcaa ttatggtttc tccgccaaga atctctttgc atttatcaag
     5701 tgtttccttc attgatatcc cgagagcatc aacatgcaat gttgtaggga tggcaatttt
     5761 tacgcctgtt ttgctttgtt cgacataaag atatccatct acgatatcag accacttcat
     5821 ttcgcataaa tcaccaactc gctgcccggt aacaacagcc agttccattg caagtctgag
     5881 ccaacatggt gatgattctg ctgcttgata aattttcagg tattcgtcag ccgtaagtct
     5941 tgatctcctt acctctgatt ttgctgcgcg agtggcagcg accgggtttg ttgttatatg
     6001 gccttcagct attgcctctc ggaatgcatc gctcagtgtt gatctgatta acttggctga
     6061 cgccgccttg ccctcgtcta tgtatccatt gagcattgcc gcaatttctt ttgtggtgat
     6121 gtcttcaagt ggagcatcag gcagccccct ccttattgct ttaattttgc tcatgtaatt
     6181 tatgagtgtc ttctgcttga ttcctctgct ggcgaggatt ttttcgtagc gatcaagcca
     6241 tgaatgtaac gtaacagaat tatcactgtt gattctcgct gtcagaggct tgtgtttgtg
     6301 tcctgaaaat aactcaatat tggcctgtat tgcttcagtg attgctatcc tcctgtctcg
     6361 gcctaaacca aactctttac ccgtccttgg gtccctgtag cagtaatatc cattgtttct
     6421 tatataaagg ttagggggta aatcccggcg ctcatgactt cgccttcttc ccatttctga
     6481 tcctcttcaa aaggctacct gttactggtc gatttaagtc aacctttacc gctgattcgt
     6541 ggaacagata ctctcttcca tccttaaccg gaggagggaa tatcctgcat tcgcgcaccc
     6601 atcgacgaac tgtttcaagg cttcttgggc gtcgctggcg tgcgttccac tcctgaagtg
     6661 tcaagtacat cgcaaagtct ccgcaattac acgcaagaaa aagccgccat caggcggctt
     6721 ggtgttcttt cagttcttca attcgaatat tggttatgtc tgcatgtgct atctgcgccc
     6781 atatcatcca gtggtcatag cagtcattga tgttctctgc ttcgataact ctgttgaatg
     6841 gttctccatt ccattcacct gtgactcgga agtgcattta tcatctccat aaaacaaaac
     6901 ccgccgtagc gagttcagat aaaagaaatc ctcgtcagtg cgaggatgct gttcattgct
     6961 gctatacact tttttgctct caacgtaagc ggtagctcat tctgttgggt tggtgcagtt
     7021 gcttttagga aatgctattt accccttaaa cgtcggctga aagagctaaa atccatgcaa
     7081 aaaatttacg caattttgtg tattattgtg cagtaagtaa tgagctattt tctgcgcaaa
     7141 aaatggatgg taaatttgtc cggggcagga aaaattttat ggacgctaaa catgaaaaaa
     7201 gattcgtatc cttatttgat ttgcatgaca gtttcaggcc tgatctttat tttccttttc
     7261 ttctggtggc gggcagatat ttacagggtc acgtttctta atcagagtat atcccactat
     7321 tacattctgt ttagcatggg aatagctttt ctgttatctc tgttttgggt taagaagggg
     7381 atagtaaaac aaagcggctg gaagagtctg tcagcatacc ttaaggttta tgcagggatg
     7441 tgcatatttg ctggattttt tctgattata ccccttacaa cactaactta ttttttgcct
     7501 ggagagacat cgtcttatgt tgcaccgtat cggtatactt ccggtagttc aaaaagttgt
     7561 tctggagctg aggtggatga ccccgatcta catgagaata ttcgcatttg ctatccgtat
     7621 ggcaattatg agtacgataa tattatctat gttgaaaaga aaattaatac attaggtgcg
     7681 gtagtaacat atgcacagac cgcgcgtgat gatactgaat gagatagtat atagcgggca
     7741 agttttagtt aatttatcga ggtaatataa tttacctcga ctcgtttatt ctggtattaa
     7801 tatttcgctt tacgatcgat ttttatctga tgatatcatg cggttttcat atactgactt
     7861 actgtctttt ctccgttagc gattttctcc tgctcagcga tgattttatc tttggcttct
     7921 agttaatttc gctcacttcg aacctctctg tttactgata agttccagat cctcctggca
     7981 acttgcacaa gtccgacaac cctgaacggc caggcgtctt cgctcatcta t
//
LOCUS       DADSYM010000177         7921 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2415
            SAMN08039579-rid11723173.denovo.179, whole genome shotgun sequence.
ACCESSION   DADSYM010000177 DADSYM010000000
VERSION     DADSYM010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039579
            Sequence Read Archive: SRR6362533
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7921)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7921)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980162.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 03:39:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,484
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 4,347
            CDSs (with protein)               :: 4,347
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 43 of 85
            Pseudo Genes (incomplete)         :: 37 of 85
            Pseudo Genes (internal stop)      :: 19 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7921
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039579-rid11723173.denovo.179"
                     /strain="LGE2415"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8302 N 95.9628 W"
                     /collection_date="2015-08-24"
     gene            complement(93..950)
                     /gene="murI"
                     /locus_tag="J1Y83_003889"
     CDS             complement(93..950)
                     /gene="murI"
                     /locus_tag="J1Y83_003889"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HBA7631992.1"
                     /translation="MATKLQDGNTPCLAATPSEPRPTVLVFDSGVGGLSVYDEIRHLL
                     PDLHYIYAFDNVAFPYGEKSEVFIVERVVEIVTAVQERYPLALAVVACNTASTVSLPA
                     LREKFDFPVVGVVPAIKPAARLTANGIVGLLATRGTVKRSYTHELIARFANECQIEML
                     GSAEMVELAEAKLHGEDVSLDALKRILRPWLRMKEPPDTVVLGCTHFPLLQEELLQVL
                     PEGTRLVDSGAAIARRTAWLLEHEAPDAKSADANIAFCMAMTPEAEQLLPVLQRYGFE
                     TLEKLAVLG"
     gene            complement(895..2739)
                     /gene="btuB"
                     /locus_tag="J1Y83_003890"
     CDS             complement(895..2739)
                     /gene="btuB"
                     /locus_tag="J1Y83_003890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent vitamin B12 receptor BtuB"
                     /protein_id="HBA7631993.1"
                     /translation="MIKKASLLTACSVTAFSAWAQDTSPDTLVVTANRFEQPRSTVLA
                     PTTVVTRQDIDRWQSTSVNDVLRRLPGVDITQNGGSGQLSSIFIRGTNASHVLVLIDG
                     VRLNLAGVSGSADLSQFPIALVQRVEYIRGPRSAVYGSDAIGGVVNIITTRDHPGTEI
                     SAGWGSNSYQNYDVSTQQQLGDKTRVTLLGDYAHTHGYDVVAYGNTGTQAQPDNDGFL
                     SKTLYGALEHNFTDVWSGFVRGYGYDNRTNYDAYYSPGLPLVDTRKLYSQSWDAGLRY
                     NGELIKSQLITSYSHSKDYNYDPHYGRYDSSATLDEMKQYTVQWANNIIIGHGNIGAG
                     VDWQKQSTAPGTAYVEDGYDQRNTGIYLTGLQQVGDFTFEGAGRSDDNSQFGRHGTWQ
                     TSAGWEFIEGYRFIASYGTSYKAPNLGQLYGSYGNPNLNPEKSKQWEGAFEGLTAGVN
                     WRISGYRNDVSDLIDYDDHTLKYYNEGKARIKGVEATANFDTGPLTHTVSYDYVDARN
                     AITDTPLLRRAKQQVKYQLDWQLYDFDWGITYQYLGTRYDKDYSSYPYQTVKMGGVSL
                     WDLAVAYPVTSHLTVRGKIANLFDKDYETVYGYQTAGREYTLSGSYTF"
     regulatory      complement(2804..2994)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            3108..4208
                     /gene="trmA"
                     /locus_tag="J1Y83_003891"
     CDS             3108..4208
                     /gene="trmA"
                     /locus_tag="J1Y83_003891"
                     /EC_number="2.1.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (uridine(54)-C5)-methyltransferase TrmA"
                     /protein_id="HBA7631994.1"
                     /translation="MTPEHLPTEQYEAQLAEKVVRLQSMMAPFSDLVPEVFRSPVSHY
                     RMRAEFRIWHDGDDLYHIIFDQQTKSRIRVDSFPAASELINQLMTAMIAGVRNNPILR
                     HKLFQIDYLTTLSNQAVVSLLYHKKLDDEWRQQAEALRDALRAQNLNVHLIGRATKTK
                     IALDQDYIDERLPIAGKEMIYRQVENSFTQPNAAMNIQMLEWALDVTKGSKGDLLELY
                     CGNGNFSLALARNFDRVLATEIAKPSVAAAQYNIAANHIDNVQIIRMAAEEFTQAMNG
                     VREFNRLQGIDLKSYQCETIFVDPPRSGLDSETEKMVQAYPRILYISCNPETLCKNLE
                     TLSQTHKVERLALFDQFPYTHHMECGVLLTAK"
     gene            complement(4248..4607)
                     /locus_tag="J1Y83_003892"
     CDS             complement(4248..4607)
                     /locus_tag="J1Y83_003892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YijD family membrane protein"
                     /protein_id="HBA7631995.1"
                     /translation="MKQANQDRGTLLLALVAGLSINGTFAALFSSIVPFSVFPIISLV
                     LTVYCLHQRYLNRTMPVGLPGLAAACFILGVLLYSTVVRAEYPDIGSNFFPAVLSVIM
                     VFWIGAKMRNRKQEVAE"
     gene            complement(4607..5257)
                     /gene="fabR"
                     /locus_tag="J1Y83_003893"
     CDS             complement(4607..5257)
                     /gene="fabR"
                     /locus_tag="J1Y83_003893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002460670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor FabR"
                     /protein_id="HBA7631996.1"
                     /translation="MMGVRAQQKEKTRRSLVEAAFSQLSAERSFASLSLREVAREAGI
                     APTSFYRHFRDVDELGLTMVDESGLMLRQLMRQARQRIAKGGSVIRTSVSTFMEFIGN
                     NPNAFRLLLRERSGTSAAFRAAVAREIQHFIAELADYLELENHMPRAFTEAQAEAMVT
                     IVFSAGAEALDVGVEQRRQLEERLVLQLRMISKGAYYWYRREQEKTTIIPGNVKDE"
     gene            complement(5361..6677)
                     /locus_tag="J1Y83_003894"
     CDS             complement(5361..6677)
                     /locus_tag="J1Y83_003894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBA7631997.1"
                     /translation="MTAIVNETVSVKKKPSGRRVIFASAFGNALEFFDFGVYNFFVVY
                     ISTLFFPPSADHNVALLLAFATFGVSFFMRPLGGIIVGAWADRFGRKPAMVFTIALMS
                     LGTLMIGIAPTYETAGYWGTATLVLARLIQGVAAGGEVGASMSLLVESAPANRRGFYS
                     SWSLATQGLATTFGGVVALGLSAWLPFATGSETVMAEWGWRVPFFIGVLLAPIGCWLR
                     LSLENDVPEPVRNKKAATSESAFSLLLQHKATIVNGVLLAIGSTVATYISLFYYGTWA
                     AKYLAMPQHYSHAAMLLAGVITFVGALLVGMLCDSVGRKKLILISRVMVLLCSWPSFW
                     LLVNYPSPGMLLTVVFVMVSFTTLGGVPVMLLISELLPKRIRALGFALVYSIGVAIFG
                     GFAQYFATQSIVLLDSLTAPAWYLGGGTLLSMLALLYVKEPAKELQ"
     gene            complement(6720..7886)
                     /locus_tag="J1Y83_003895"
     CDS             complement(6720..7886)
                     /locus_tag="J1Y83_003895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HBA7631998.1"
                     /translation="MIEHYIRGFEEDLREIRHQIHENPELGLQEFKTSALVAEKLRQW
                     GYEVEQGLATTGVVATLKVGDGEKSIGLRADMDALPIYENSGKPWASKHPGLMHACGH
                     DGHTTILLGAARYFAETRRFNGTLRLIFQPAEEMINGGEIMVKEGLFDRFPCDVIFGM
                     HNMPGLPVGKFFFQPGALMASMDQFHITVRGCGGHGAIPHKAIDPVLVAAHITTALQS
                     IVSRNVDPLEAAVITVGSIVAGEAANVIPDSAEMKISVRSLSRDTRQLLLTRIPALAQ
                     AQAASFGATAEVTHVNGTPVLVNDEEMARFAWQVACKTFGEDRAEFGIKPLMGSEDFS
                     FMLEAQPKGGFLLFGNGDVGEGSCMVHNPGYDFNDASLVPASSYWGALVEAWLQ"
BASE COUNT         2093 a         2158 c         1872 g         1798 t
ORIGIN      
        1 tccgtgtcag tggtggcgca ttatagggag ttattccggc ctgacaagag gaaatttaaa
       61 ataattttct gaccgcgcaa cattcaacca aatcagccta aaactgccag tttttcgagc
      121 gtttcgaagc cgtaacgctg taaaacgggc aataattgtt cagcttctgg cgtcattgcc
      181 atacaaaagg caatattcgc atcggcagat tttgcatccg gggcttcatg ttctaacagc
      241 caggccgttc ggcgagcaat cgctgcgcca gaatccacca gccgggttcc ctctggcagc
      301 acctgtaaca gttcttcttg tagtagaggg aaatgggtac accccagtac aacggtatct
      361 ggcggctctt tcattcttaa ccacgggcgt aggatacgtt ttagcgcatc cagagaaaca
      421 tcttcgccat gcagcttcgc ttcagccaac tcaaccatct ctgccgagcc cagcatttct
      481 atctggcatt cattagcgaa acgcgcgatc agctcatgag tataagaacg tttaacagtt
      541 ccgcgggttg ccagtaatcc gacaatgcca tttgccgtca gacgtgcagc aggtttaatc
      601 gccggcacga caccaacaac cgggaagtcg aacttttcgc gtaatgcagg aagtgaaacg
      661 gtactggcgg tgttgcaagc gaccacagcc agcgcaaggg gataacgttc ttgcactgcg
      721 gtgacaattt ccaccactcg ctcaacaata aacacttcgc ttttttcgcc atacgggaaa
      781 gcgacgttat cgaaagcata aatgtaatgg agatccggta agagatgccg gatctcgtca
      841 tagaccgaca acccaccgac gccggagtca aacaccagca cggtgggacg tggttcagaa
      901 ggtgtagctg ccagacaagg tgtattcccg tcctgcagtt tggtagccat agactgtctc
      961 gtaatctttg tcgaacaggt tggctatttt accacgaact gtcaggtgag aggtgaccgg
     1021 atacgcaacc gcaagatccc acaagctcac accgcccatt ttcactgttt gatacggata
     1081 agaggagtaa tccttatcat agcgagtgcc taaatactga taagtaatcc cccagtcgaa
     1141 gtcatacaac tgccagtcga gctggtattt cacctgctgt ttagcacggc gtaacaacgg
     1201 cgtgtcagta atcgcattgc gcgcatcaac ataatcataa ctcacagtat gcgtcagtgg
     1261 tccggtatca aaattagcgg tcgcctcgac acccttaatc cgcgctttcc cttcgttgta
     1321 atatttcagg gtgtgatcat cataatcgat caagtcactg acatcgttac gatatccgga
     1381 aatacgccag ttaaccccag cagttaagcc ttcaaacgcg ccttcccatt gtttgctttt
     1441 ctcaggattc agattcggat taccgtagga gccgtacagt tgcccaagat ttggtgcctt
     1501 ataagatgtt ccgtaggaag caataaagcg ataaccttcg atgaactccc aaccggcgct
     1561 ggtttgccag gtaccatgac gaccaaactg agaattatcg tcgctgcgtc ctgcgccttc
     1621 aaaagtaaaa tcgccgactt gttgcagccc agtcagatag atgccggtat tacgttgatc
     1681 atatccatcc tcaacataag ctgtgcccgg tgccgtgctc tgcttctgcc agtcaacacc
     1741 cgcaccaata ttaccgtggc caatgatgat gttgtttgcc cactggacgg tgtactgttt
     1801 catctcatcg agcgtcgcgg acgaatcata acgaccataa tgcggatcgt agttgtaatc
     1861 tttgctatgg ctgtagctgg taatcagttg tgatttaatc agttcgccgt tatagcgcag
     1921 tccggcatcc caactttggc tatagagttt acgggtatcg accagtggca agccgggaga
     1981 ataatatgcg tcataattgg tacgattatc atagccatag ccgcgcacaa agccgctcca
     2041 gacatcagta aaattatgct ccagcgcgcc ataaagcgtt ttacttaaaa aaccatcgtt
     2101 atctggctgc gcttgcgttc cggtattacc ataggcaaca acatcataac catgagtatg
     2161 ggcataatcg cccaacagcg ttacccgtgt cttatccccc agttgttgct gtgtagagac
     2221 atcatagttc tgataactat tgcttcccca ccctgctgaa atttccgttc cggggtgatc
     2281 gcgcgtcgtg atgatattca cgaccccgcc tattgcatct gaaccataaa cagcggagcg
     2341 cggcccacgg atatattcaa cacgctggac aagcgcaata gggaactgac taagatcagc
     2401 agaaccactc acccccgcca aattcaggcg gacgccatca attaacacca acacatgact
     2461 ggcatttgta ccgcgaataa aaatagatga gagctgacct gaaccgccgt tttgggtgat
     2521 atccacgccc ggaagacggc gcagcacatc attgaccgag gtcgactgcc agcggtcgat
     2581 atcctgacgc gtcacaacag tagttggcgc aagcacagtg ctgcgcggtt gttcaaaacg
     2641 gttagcagta acgacgagag tatccgggct ggtatcctgt gcccaagcgg aaaatgccgt
     2701 gacggaacac gccgtcagca gcgaagcttt tttaatcatt gtaaagcatc cacaatagaa
     2761 gaaggatgcc gcaggtttca tcaatattac gcgatgatga gaaccagatg cgacgttggc
     2821 cggcaggtct tcgggcttgg aggggtatct aagatactaa gagatgatga cttcccaccg
     2881 gatggcagtg tccgcataac gcaatcatcg cacctttcct taccgctgcg cgtcagctcc
     2941 agattcgcac tggattccct attaactcac aggaccggca agtggatgct acaggttgta
     3001 acaagttact gtccagacgt agctcacaaa taggaattca tcaagatctg gacatctgat
     3061 gagcaatccc tacaatcgcc gcgtacttta atttttcagg atacatcatg acccccgaac
     3121 accttccaac agaacagtat gaagcgcagt tagccgaaaa agtggtacgt ttgcaaagta
     3181 tgatggcacc gttttctgac ctggttccgg aagtgtttcg ctcgccggtc agtcattatc
     3241 ggatgcgtgc ggagttccgc atctggcacg atggcgatga cctgtaccac atcattttcg
     3301 atcaacaaac caaaagtcgt atccgtgtgg atagcttccc cgccgccagt gaacttatca
     3361 accagttgat gacggcgatg attgcgggtg tgcgtaataa tcccattctg cgccacaagt
     3421 tgttccagat tgattacctc actacgctaa gcaatcaagc ggttgtttcc ttgctgtacc
     3481 ataaaaagct ggatgatgag tggcgtcagc aggcggaagc cctgcgcgat gcactgcgcg
     3541 cgcagaatct gaatgtgcat ctgattggtc gggcaacgaa aaccaaaatc gcgctggatc
     3601 aggattacat cgacgaacgt ctgccgatcg cggggaaaga gatgatctac cgtcaggtag
     3661 aaaacagctt tacccaaccg aacgcggcga tgaatattca gatgctggaa tgggcgctgg
     3721 acgtaaccaa aggctcaaaa ggcgatttac tggagctata ctgcggcaac ggtaactttt
     3781 cattagcgct ggcacgcaat tttgatcggg tattagccac cgaaatcgcc aagccgtcag
     3841 tcgccgccgc gcaatacaac attgccgcta accatattga taacgtacaa attattcgta
     3901 tggcggcaga agaatttact caggcgatga atggtgtgcg cgagtttaac cgcctgcaag
     3961 gaatcgactt aaagagttat cagtgcgaaa ccatttttgt cgacccaccg cgcagcggtc
     4021 tggacagtga aaccgagaaa atggtgcagg cgtatccacg tattctgtat atctcctgca
     4081 atccggaaac gttatgcaag aatctggaaa cattaagcca gacgcacaag gtcgaacgtc
     4141 tggctctatt tgatcagttc ccctacacgc accatatgga gtgcggcgta ttactgaccg
     4201 cgaagtaaaa cccatgccgg atgcgcttgc atccggcatg ataccgatta ctcagcaact
     4261 tcctgcttac ggttacgcat cttcgcgcca atccagaaca ccataatgac ggacagtact
     4321 gccgggaaga agttagagcc gatatccgga tattccgcac gaactaccgt gctgtacagc
     4381 agtacgccga gaataaaaca agcagctgcc agacccggca agcctaccgg catagtgcga
     4441 ttaagataac gttgatgcag gcagtaaact gtcaacacca gggaaataat cgggaataca
     4501 gaaaatggta caatggagct aaacagcgct gcaaaagtac cattgatcga taagccagca
     4561 accaacgcta gcagcagcgt acctctatct tgatttgctt gtttcattac tcgtccttca
     4621 catttcccgg aataattgtg gttttctctt gttcacggcg ataccagtaa taagcccctt
     4681 tcgaaatcat tcgcagctgc agtaccagtc gctcttctaa ttgccggcgt tgctcgacgc
     4741 cgacatccaa cgcctcggcc cccgcactga agacaatcgt caccattgct tcggcttgcg
     4801 cttcagtaaa cgcacgcggc atatggtttt cgagttccag atagtccgca agttccgcaa
     4861 tgaagtgctg aatttcacgc gcaacggcgg cacgaaacgc agcggaggtg ccggagcgtt
     4921 cccgcaataa taaccggaag gcgttaggat tattaccgat gaactccata aatgtggaga
     4981 ccgaggtgcg gatcacactc ccgcctttgg cgatacgctg acgcgcctga cgcatgagtt
     5041 ggcgtagcat taaaccgctc tcatcaacca tggtcagacc cagttcgtct acgtcgcgga
     5101 aatgccgata aaaagaggtg ggagcaatgc ctgcttctcg tgccacttca cgcaaactca
     5161 ggctggcgaa gctgcgttca gcacttaatt ggctaaatgc ggcttccacc agcgaacggc
     5221 gggttttttc tttttgttgc gctcttacgc ccatcacgat gtctgaatcc ttgccaaaag
     5281 tactgctggc actataccag agaatgaaca tactggattg ctccagtgtt gtgaatgaac
     5341 ggtaacgtaa taaaacatat ttactgcaat tctttggccg gttcttttac gtacaacaac
     5401 gccagcatcg acagcaacgt gcctcccccc agataccacg ccggagccgt cagactatcc
     5461 agcaacacga tggattgcgt ggcgaaatat tgcgcaaaac cgccaaagat tgctacgcca
     5521 atgctataaa ccagcgcgaa acctaatgcc cgaatccgtt tcggcaacag ttcagagatc
     5581 aacaacatca ctggcacacc gccaagcgtg gtaaagctga ccatcacgaa aaccaccgtc
     5641 agcaacatgc cagggcttgg gtaattcacc aacagccaga atgacggcca gctacagaga
     5701 agcaccatca cacgggagat taaaatcagc tttttacgcc caaccgaatc acatagcatc
     5761 cccaccaaca gcgcacccac aaacgtaatt acccccgcca gtaacatcgc tgcatgagaa
     5821 taatgttgag gcatcgccag atacttcgcc gcccaggtgc cgtaataaaa gagggagata
     5881 taggttgcga ccgtgctacc aatagcgagt aaaacaccat taacgatagt cgccttatgt
     5941 tgcaggagca gagaaaacgc gctttcgctg gtagcagctt ttttattgcg cactggctca
     6001 ggtacgtcat tttccaggct cagacgcaac cagcaaccga taggtgctaa taacacgcca
     6061 ataaaaaacg gcacacgcca gccccattct gccattacgg tttctgaacc tgtggcgaac
     6121 ggtagccatg cgctcagacc taaagcaaca accccaccga aggtcgttgc cagcccttgg
     6181 gtcgccagtg accaactgct gtaaaagccg cgacgatttg ccggagctga ctccaccagt
     6241 agcgacattg aagccccgac ttcaccacct gccgcaacgc cctgaattag acgcgccagc
     6301 accagcgttg cagttcccca atagcccgcc gtttcatacg tcggtgcgat gccgatcatc
     6361 agtgtaccga ggctcatcag agcaatagtg aacaccatcg ccggtttacg cccgaagcga
     6421 tcggcccagg caccaacgat aatcccaccg agcggacgca taaaaaagct cacaccaaac
     6481 gttgcaaatg ccagtaacag cgcgacgtta tgatcggcac tgggtgggaa gaaaagcgta
     6541 ctgatataga ccacaaaaaa gttgtaaacg ccaaaatcaa aaaattcgag cgcattgcca
     6601 aacgccgagg caaagatcac ccgcctgccg ctgggctttt tcttcacact cacggtttca
     6661 tttacgatcg ccgtcattcc atgttccttg ccaaaaagtg ttgttgtgtg ttgttttatt
     6721 tattgcagcc aggcttcaac cagtgcgccc cagtagctgc ttgccgggac caggcttgca
     6781 tcgttaaagt cgtaaccggg gttatgcacc atgcaggaac cttccccgac atcgccatta
     6841 ccgaataaca ggaaaccgcc tttgggttgg gcttccagca tgaaagagaa atcttcactg
     6901 cccatcaggg gtttgatgcc aaactcagcc cgatcttcac caaatgtttt gcacgccacc
     6961 tgccaggcga agcgcgccat ctcctcgtca tttactaata ccggtgtacc gttaacatgc
     7021 gtaacctcag ccgtagcgcc aaagctggca gcctgggctt gtgcaagagc aggaatacgg
     7081 gtcagaagaa gttgtcgggt atcgcggcta agagagcgga cactgatttt catctcagca
     7141 ctgtcaggaa tgacgttagc cgcctcaccc gcaacaatgc tgccgacggt aattaccgcc
     7201 gcttccagcg gatcgacatt gcgcgacaca atgctttgta atgcggtggt gatatgtgcg
     7261 gcaaccagca ctggatcgat ggctttgtgc gggatcgcac cgtgtccgcc acaaccacga
     7321 accgtaatat ggaactgatc cattgacgcc atcagcgccc caggctggaa gaaaaacttg
     7381 ccgaccggca aacccggcat gttgtgcatc ccgaaaatga catcgcaggg gaaacgatca
     7441 aaaagcccct ctttaaccat gatctcaccg ccgttaatca tctcttccgc aggctggaag
     7501 ataaggcgta acgtgccgtt aaaacggcgg gtttcggcaa aataacgcgc agcccccagc
     7561 agtatggtgg tatgcccgtc atgcccgcag gcgtgcatta aaccaggatg tttactggcc
     7621 cagggcttgc cactattttc ataaatcggc aacgcatcca tatccgcccg caggccaata
     7681 cttttctctc cgtcgcccac ttttaacgtg gctaccacgc cggttgtcgc cagcccttgc
     7741 tcaacctcat agccccactg gcgcagcttt tccgcaacca aagcactggt tttaaattct
     7801 tgtaatccca gctccgggtt ttcgtggatc tgatggcgga tctcgcgaag gtcttcttca
     7861 aacccacgaa tatagtgttc gatcatcggc tattccttta ttcacttata aacaggctaa
     7921 t
//
LOCUS       DADSYM010000178         7849 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2415
            SAMN08039579-rid11723173.denovo.180, whole genome shotgun sequence.
ACCESSION   DADSYM010000178 DADSYM010000000
VERSION     DADSYM010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039579
            Sequence Read Archive: SRR6362533
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7849)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7849)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980162.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 03:39:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,484
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 4,347
            CDSs (with protein)               :: 4,347
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 43 of 85
            Pseudo Genes (incomplete)         :: 37 of 85
            Pseudo Genes (internal stop)      :: 19 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7849
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039579-rid11723173.denovo.180"
                     /strain="LGE2415"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8302 N 95.9628 W"
                     /collection_date="2015-08-24"
     gene            23..634
                     /locus_tag="J1Y83_003896"
     CDS             23..634
                     /locus_tag="J1Y83_003896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016245973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPASM domain-containing protein"
                     /protein_id="HBA7631999.1"
                     /translation="MQFIELLETGTPNIDFSGHSENTFRIIDFSVPPTAYGKFMSTIF
                     MRWVKNDVGEIFIRQFESFVSRFLGNGHTSCIFQESCKDNLVVESNGDIYECDHFVYP
                     QYKIGNINKSELKTMNSVQLTAQKKRISAKCQQCVYKPICNGGCPKHRITKVNNETVS
                     YFCEGYKILFSTMVPYMNAMVELAKNRVPLYHIMDVAKQMENN"
     gene            complement(600..725)
                     /locus_tag="J1Y83_003897"
     CDS             complement(600..725)
                     /locus_tag="J1Y83_003897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA7632000.1"
                     /translation="MKTKHPTGIYVGCLVKVIRRRLRMELRGSVINYSPFVLQHP"
     gene            925..2610
                     /locus_tag="J1Y83_003898"
     CDS             925..2610
                     /locus_tag="J1Y83_003898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
                     /protein_id="HBA7632001.1"
                     /translation="MITIPITFCMLIAKYLCLLKPFWLRKNNKTSVLLIIIILAMILG
                     VVKIQVWLNDWNNDFFNALSQKETDKLWQLVLWFPALLGIFVLISVNKTWLIKLLTIR
                     WREWLTDYYLNRWFADKNYYFTQIYGEHKNTDNPDQRIAEDILLLISKTLSLSFGFIQ
                     SLSMLITFTVILWQSAGTLSFTVGGTEWSIQGYMVYTVVLIVIGGTLFTHKVGKRIRP
                     LNVEKQRSEATFRTNLVQHNKQAELIALSNAESLQRQELSENFHTIKENWHRLMNRQR
                     WLDYWQNIYSRSLSVLPYFLLLPQFISGQINLGGLMKSRQAFMLVSNNLSWFIYKYDE
                     LAELAAVIDRLYEFHQLTEQRPTNKPKNCQHAVQVADASIRTPDNKIILENLNFHVSP
                     GKWLLLKGYSGAGKTTLLKTLSHCWPWFKGDISSPADSWYVSQTPLIKTGLLKEIICK
                     ALSLSVDDKSLSEVLHQVGLGKLAARIHDHDRWGDILSSGEKQRIALARLILRRPKWI
                     FLDETTSHLEEQEAIRLLRLVREKLPTSGVIMVTHQPGVWNLVDDICDISAVI"
     gene            2648..5020
                     /locus_tag="J1Y83_003899"
     CDS             2648..5020
                     /locus_tag="J1Y83_003899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HBA7632002.1"
                     /translation="MKRVLIPGVILCGADVAQAVDDKNMYMHVFEEMTVYAPVPVPVN
                     GNTHYTSESIERLPTGNGNISDLLRTNPAVRMDSTQSTSLNQGDIRPEKISIHGASPY
                     QNAYLIDGISATNNLNPANESDASSATNISGMSQGYYLDVSLLDNVTLYDSFVPVEFG
                     RFNGGVIDAKIKRFNADDSSVKLGYRTTRSDWLTSHIDENNKSAFNQGSSGSTYYSPD
                     FKKNFYTLSFNQELADNFGVTAGLSRRQSDITRADYVSNDGIVAGRAQYKNVIDTALS
                     KFTWFASDRFTHDLTLKYTGSSRDYNTSTFPESDREMGNKSYGLAWDMDTQLAWAKLR
                     TTVGWDHISDYTRHDHDIWYTELSCTYGDISGRCTRGGLGHISQAVDNYTFKTRLDWQ
                     KFAVGNVSHQPYFGAEYIYSDAWTERHNQSESYVINAAGKKTNHTIYHKGKGSLGIDN
                     YTLYMADRISWRNVSLMPGVRYDYDNYLSNHNISPRFMTEWDIFADQTSMITAGYNRY
                     YGGNILDMGLRDIRNSWTESVSGNKTLTRYQDLKTPYNDELAMGLQQKIGKNVIARAN
                     YVYREAHDQISKSSRTDSATKTTITEYNNDGKTKTHSFNLSFELAEPLHISQVDINPQ
                     IVFSYIKSKGNLSLNNGYEESNTGDNRVVYNGNLVSYDSVPVADFNNPLKISLNMDFT
                     HQPSGLVWANTLTWQEARKARIILGKTNAQYISEYSDYKQYVDEKLDSSLTWDTRLSW
                     TPQFLKQQNLTISADILNVLDSKTAVDTTNTGVATYASGRTFWLDVSMKF"
     gene            5065..>7849
                     /locus_tag="J1Y83_003900"
     CDS             5065..>7849
                     /locus_tag="J1Y83_003900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HBA7632003.1"
                     /translation="MEIIMRNLCFLLTLVATLLLPGRLIAAALPQDEKLITGQLDNGL
                     RYMIYPHAQPKDQVNLWLQIHTGSLQEEDNERGVAHFVEHMMFNGTKTWPGNKVIETF
                     ESMGLRFGRDVNAYTSYDETVYQVSLPTTQKQNLQQVMAIFSEWSNAATFEKLEVDAE
                     RGVITEEWRAHQDAKWRTSQARRPFLLANTRNLDREPIGLMDTVATVTPAQLRQFYQR
                     WYQPNNMTFIVVGDIDSKEALALIKDNLSKLPANKAAENRVWPTKAENHLRFNIINDK
                     ENRVNGIALYYRLPMVQVNDEQSFIEQAEWSMLVQLFNQRLQERIQSGELKTISGGTA
                     RSVKIAPDYQSLFFRVNARDDNMQDAANALMAELATIDQHGFSAEELDDVKSTRLTWL
                     KNAVDKQAERDLRMLTSRLASSSLNNTPFLSPEETYQLSKRLWQQITVQSLAEKWQQL
                     RKNQDAFWEQMVNNEVAAKKALSPAAILALEKEYANKKLAAYIFPGRNLSLTVDADPQ
                     AEISSKETLAENLTSLTFSNGARVILAKSAGEEQKLQIIAVSNKGDLSFPAQQKSLIA
                     LANKAVSGSGVGELSSSSLKRWSAENSVTMSSKVSGMNTLLSVSARTNNPEPGFQLIN
                     QRITHSTINDNIWASLQNAQIQALKTLDQRPAEKFAQQMYETRYADDRTKLPQENQIA
                     QFTAADALAADRQLFSSPADITFVIVGNVAEDKLVALITRYLGSIKHSNSPLAAGKPL
                     TRATDNASVTVKEQNEPVAQVSQWKRYNSRTPVNLATRMALDAFNVALAKDLRINIRE
                     QASGVYSVSSRLSVDPQAKDISHLLAFTCQPERHDELLTLANEVMVKRLAKGISEQEL
                     NEYQQNVQRSLDIQQRSVQQLANTIVNSLIQYDDPAAWTEQEQLLKQMTVENVNTAVK
                     QYLSHPVNTYTGVL"
BASE COUNT         2270 a         1730 c         1767 g         2082 t
ORIGIN      
        1 aaaatctatc ggcagtaaac atatgcaatt tatcgaattg ctcgaaaccg ggacgccgaa
       61 tattgatttc agtggtcata gtgagaacac attccgtatc attgattttt ctgtgcctcc
      121 cacggcttat ggcaagttta tgtcaaccat ttttatgcga tgggttaaaa acgatgtggg
      181 tgaaattttc atccgtcagt ttgaaagctt tgtcagccgt tttttgggga atgggcatac
      241 cagttgtatt ttccaggagt cctgcaagga taatctggtt gttgaaagta atggagacat
      301 ttacgaatgc gaccattttg tctatccaca gtacaaaatt ggaaacatta ataaatcgga
      361 actcaaaacg atgaacagtg tacaactgac agcgcaaaaa aaacggattt cagccaaatg
      421 tcagcaatgt gtatataaac ctatctgcaa tggtggttgc cctaagcatc gtattactaa
      481 agtaaacaat gagactgttt cctatttttg cgaaggttat aaaatccttt tttcaaccat
      541 ggtaccttat atgaacgcca tggtggagtt agctaagaac agagtaccgc tttaccacat
      601 tatggatgtt gcaaaacaaa tggagaataa ttaataacgc tcccccttag ctccattctt
      661 agcctccttc ttatgacctt aacaaggcac ccgacataaa ttcctgtcgg gtgtttcgtc
      721 ttcatcatct tcctttgcga aaatcctggc ttacgaggta tgcgtcaggg cattagcaat
      781 acccttgatt atcaatagta taaccataac cactgaaaat taaatatttc tttatatttc
      841 atattgttaa atgatactta aataaatttc atcagtatat cattgccata gataatcatt
      901 gggtggcaca atcaattttc gctaatgata accattccca ttacgttttg tatgttgata
      961 gcaaagtatc tctgtctgct gaagcccttt tggctgcgta agaataataa aacttcagtg
     1021 ttgttaatca ttattatcct cgccatgatc cttggcgtgg ttaaaattca ggtctggtta
     1081 aacgactgga ataatgattt tttcaatgcg ctaagccaga aagaaactga taagctttgg
     1141 cagcttgtcc tttggttccc tgccctgctg gggatctttg tattaatatc cgtaaacaaa
     1201 acatggttaa ttaaactact gactattcgc tggcgcgaat ggttaacaga ttactacctt
     1261 aatcgctggt ttgcagataa aaactactac ttcacgcaga tctacggcga gcataaaaat
     1321 acagataacc ctgaccaacg tatcgctgaa gacattctct tactgatcag caaaacgctc
     1381 agcctttcat ttggcttcat ccagtcactt agcatgctga tcacctttac cgttattctt
     1441 tggcaaagcg cgggtacgct ctcattcact gttggtggaa ctgaatggag tatccagggg
     1501 tatatggtct ataccgtcgt gctcattgtg atcggtggaa ctttatttac ccataaggtt
     1561 ggtaaacgta ttcgtccact taatgtggaa aaacaacgta gcgaagcgac atttcgaact
     1621 aatcttgtgc agcataataa gcaggcagaa ctgattgcgc taagcaatgc tgagtctcta
     1681 caacgccagg agttgagcga aaattttcat accattaaag agaactggca tcgtttaatg
     1741 aatcgtcagc ggtggcttga ttactggcag aatatttatt cgcgctcgct tagcgttctt
     1801 ccctactttc tgttattgcc gcagtttatt agcgggcaga tcaatctggg cggactgatg
     1861 aaatcgcgcc aggcatttat gctggtatcg aacaatttaa gctggtttat ttataaatat
     1921 gatgaacttg ctgaactggc tgcggttatc gatcgcttgt atgagttcca tcaactcacc
     1981 gaacagcgcc ctacgaataa gcctaaaaat tgtcaacatg cggtacaagt ggctgacgcg
     2041 agtattcgta ctcctgataa taagatcata ttagagaacc tgaactttca tgtttcgcca
     2101 ggcaaatggc tattgctgaa aggttactct ggtgcgggaa aaaccacact gcttaaaaca
     2161 ttatcccact gctggccgtg gtttaaaggt gatatttctt ctcctgctga cagttggtat
     2221 gtgtcacaaa caccgttaat caaaaccggc ttactgaaag agattatttg taaagcactt
     2281 tccctgtccg tagacgataa atcgttgagc gaagtactgc atcaggttgg tctggggaaa
     2341 ttggctgcgc gtattcatga tcacgatcgc tggggagata ttctttccag cggcgaaaaa
     2401 caacgtatcg ccctggctcg attaatttta cgacgtccga aatggatatt tcttgacgaa
     2461 actacctctc atcttgagga acaagaggca atccgcttac tgcgtttagt gcgagaaaaa
     2521 ctccccacaa gcggcgtcat tatggttaca catcaacccg gtgtctggaa cctggtcgat
     2581 gatatttgtg acattagcgc ggttatataa aaacaataaa agccctgctg tacggagata
     2641 taaataaatg aagcgagttc ttattcctgg cgtcatttta tgtggcgctg atgtggcgca
     2701 ggccgtcgat gacaaaaata tgtacatgca tgtttttgaa gagatgacgg tctatgctcc
     2761 tgtccctgta cccgtaaacg gcaacacgca ttacaccagt gaaagcatcg agcgtttacc
     2821 gaccgggaat ggcaatatca gcgatctgct gagaaccaac cctgcggtac gcatggattc
     2881 aacgcaaagt acctcattga accagggaga tattcgcccg gagaaaatct ctattcacgg
     2941 tgcgtcgccc taccagaatg cctatttgat tgacggtatt agtgcaacga ataacctgaa
     3001 cccagcgaat gagtccgatg ccagtagtgc aaccaatatt agcgggatgt cacaggggta
     3061 ttatcttgat gtcagcttac tggacaatgt gacgctttat gacagttttg tgccagttga
     3121 attcggtcgc ttcaatggcg gggtaattga tgcaaagatc aaacgcttca acgctgatga
     3181 tagcagcgtg aaactgggtt atcgcactac gcgttcggac tggttaacat cgcatatcga
     3241 tgagaataac aagagcgcat ttaatcaagg ttcttcagga agtacttatt actctccaga
     3301 ttttaaaaag aacttttata ccttgtcgtt taatcaggaa ctcgctgata actttggcgt
     3361 taccgctggt ttatcgcgcc gccagtctga tatcacccgc gcggattatg tttcgaatga
     3421 cggcattgtc gccggcaggg cacagtataa aaacgttatc gatactgcat tgagcaaatt
     3481 tacctggttt gccagcgacc gctttaccca cgatttaacc ttaaaatata ccggctccag
     3541 ccgtgattat aataccagca ccttcccgga gtctgatcgc gaaatgggta ataaatccta
     3601 tggtctggca tgggatatgg atacccagct cgcatgggcc aaactgcgta ccaccgttgg
     3661 ttgggatcat attagtgatt atacccgtca cgatcatgac atctggtaca ccgaactttc
     3721 atgtacatat ggtgatattt cagggcgttg cacccgtggc ggattaggac acatttccca
     3781 ggctgtagat aattacacct tcaaaacacg cctggactgg caaaaattcg ccgtgggtaa
     3841 tgtttcgcat caaccctact tcggcgcgga atacatctat tccgatgcat ggactgaacg
     3901 ccataaccag tctgaatcct atgtgattaa tgctgccgga aagaaaacta accatacaat
     3961 ttaccataaa ggtaaaggta gtctgggaat tgacaattac acgctgtata tggcggatcg
     4021 cattagctgg cgtaatgtgt cgttaatgcc cggcgtgcgg tatgactatg ataactatct
     4081 gtcaaaccac aatatctccc cgcgctttat gacggaatgg gatatttttg ctgatcaaac
     4141 ctcaatgatt actgcaggtt ataaccgtta ctatggcggg aatattcttg atatgggatt
     4201 acgtgatatc cgcaatagct ggacggaatc ggtatcaggc aataaaaccc tgacgcgtta
     4261 tcaggatttg aaaacgcctt ataacgatga actggcaatg ggattgcagc agaaaatcgg
     4321 taagaacgtt attgcgcgtg caaactatgt ttaccgtgaa gcgcatgatc aaatcagcaa
     4381 aagcagtcgt accgacagcg cgactaaaac caccattact gaatataaca acgatggcaa
     4441 aaccaaaaca cattcgttta acctcagttt tgaactggcc gaacccctgc atatcagcca
     4501 ggtagatatt aacccgcaaa ttgtctttag ctatatcaag agcaagggca acttgtcgtt
     4561 aaacaatggt tatgaggaga gcaataccgg tgataaccgg gtggtttata acggtaatct
     4621 ggtctcttac gatagcgttc cagtggcgga ttttaataat ccattaaaga tctccttaaa
     4681 catggatttc acgcatcaac cgagcgggtt ggtgtgggcg aatacgctga cctggcaaga
     4741 agcgcgtaaa gctcgcatta tcctgggtaa gacgaatgcg caatacatca gcgaatattc
     4801 agattacaag cagtatgttg acgagaaact ggatagcagc ctgacctggg acacccgctt
     4861 gtcctggacg ccacaatttc tgaaacaaca aaacctgacg atcagtgccg atattctcaa
     4921 tgtactggat agcaaaaccg ctgttgatac aacgaatact ggcgtggcga cctacgccag
     4981 tggccgtact ttctggcttg atgtcagcat gaaattttaa tagttcggat tttactttac
     5041 cgggtaaatt cgcccggttt tcgcatggag ataattatga gaaacctctg tttcttactg
     5101 acgttagtgg caactctgtt gctccccggg cgactgattg ccgccgcctt accgcaggat
     5161 gaaaagttaa ttaccgggca actggacaat ggcttgcgat atatgattta tccgcatgct
     5221 caaccaaagg atcaggtaaa tttatggctg caaattcata ccggttcatt gcaggaagaa
     5281 gacaatgagc gcggcgtggc tcattttgta gaacatatga tgtttaacgg cacaaaaaca
     5341 tggccgggta ataaagtcat cgaaacattt gagtcaatgg gcctgcgttt tggtcgcgat
     5401 gttaatgcct ataccagcta tgacgaaaca gtgtatcagg tgagtttgcc gactacgcag
     5461 aaacaaaatc tgcaacaagt gatggcaatc ttcagtgaat ggagtaatgc cgcaaccttt
     5521 gaaaaactcg aagtagacgc tgaacgcggc gtaattactg aggaatggcg tgcccatcag
     5581 gatgcgaaat ggcgcacctc tcaggcgcgc cgccctttcc tgctggcaaa tacccgtaat
     5641 ttagaccgtg aacctatcgg cctgatggat accgtcgcca cggtcacacc ggcacaattg
     5701 cgccaatttt atcaacgctg gtatcaacca aataatatga cctttattgt ggtcggcgat
     5761 atcgacagta aagaagcgct ggcgctgata aaggataatt taagtaagct tccggctaac
     5821 aaagcagctg aaaatcgcgt ctggccgaca aaagccgaaa accacctgcg ctttaatatc
     5881 atcaatgata aagaaaaccg ggtgaacggc atcgcactct attatcgcct gccaatggta
     5941 caagtgaacg atgagcaaag ctttatcgaa caagctgaat ggagcatgtt ggttcagctg
     6001 ttcaaccaac gtctgcagga acgcatacag tcgggcgagt tgaagactat ttctggcggc
     6061 actgcgcgca gcgtcaaaat tgcacccgat tatcagtcgc tatttttccg tgtaaatgca
     6121 cgagacgata atatgcagga tgctgcgaat gcattaatgg cagagttggc aaccattgat
     6181 cagcatggtt tttctgctga agaactcgat gatgttaaat ctacccgcct tacctggctg
     6241 aaaaatgcgg ttgataagca agctgagcgt gatttacgta tgctgaccag tcgcctggca
     6301 tccagctcat taaataatac gccgttcttg tcgccggaag agacatatca actttcgaaa
     6361 cgtctgtggc agcaaattac cgtgcaaagt ctggcggaaa aatggcagca gttaagaaag
     6421 aaccaggacg cattttggga gcaaatggta aacaatgaag ttgccgccaa aaaagcattg
     6481 tctcctgcgg ctatcctggc gctggaaaaa gagtacgcca acaaaaagct ggcggcttac
     6541 atcttcccag gcagaaattt atcgttaaca gtagacgctg acccacaggc ggaaattagc
     6601 agcaaagaaa cgctggctga gaatctgaca tcattaacat tttccaatgg tgccagggtt
     6661 attctggcaa aatccgcggg tgaagagcaa aagctacaaa ttattgccgt atctaataaa
     6721 ggcgatttaa gtttccctgc gcagcaaaaa tcacttatcg cgctggctaa taaagcagta
     6781 agcggaagcg gcgttggtga actctcctct tccagcctga aacgctggag tgcggaaaac
     6841 tcggtaacca tgagcagtaa agtcagtggc atgaatacgt tgctctccgt tagcgcgcgg
     6901 actaataacc ctgaacctgg tttccagttg attaaccagc gaatcaccca cagcacgatt
     6961 aacgacaata tttgggcatc gctgcaaaat gctcaaattc aggcgttgaa aacgctcgac
     7021 cagcgtccag cggagaaatt cgcccagcag atgtatgaga cgcgctatgc tgatgaccgc
     7081 acgaaattac cgcaagaaaa tcagattgca cagtttactg ccgcagatgc actggctgcc
     7141 gatcgccagt tgttttcatc tccagcggat atcacgtttg tcattgtcgg taatgtcgca
     7201 gaagacaaac tcgtggcgtt aattacgcgt tacttaggat ctatcaaaca ctctaattcg
     7261 ccattagccg caggtaaacc attaactcgc gcgacggaca acgcatcggt tactgtaaaa
     7321 gaacaaaatg aaccagtggc acaggtttca cagtggaagc gttataattc ccggactcct
     7381 gttaatctgg cgacgcgtat ggcgctcgat gcttttaacg tcgcactggc aaaagatcta
     7441 cgcattaata ttcgtgaaca ggcatctggg gtatacagcg tttcttctcg cctctcggtt
     7501 gatcctcagg ccaaagatat cagtcatttg ctggctttta cttgtcaacc agaacgacat
     7561 gatgaactgt taacgttagc gaatgaagtg atggttaagc gtctggccaa agggattagt
     7621 gagcaagaac tgaacgaata ccagcaaaac gttcagcgca gcctcgatat ccaacagcgt
     7681 agcgttcaac aattagcgaa taccattgta aatagtctta ttcaatatga cgatcctgca
     7741 gcatggactg agcaggagca attgttgaaa caaatgacgg tagagaatgt taacactgct
     7801 gttaaacaat atctttctca tccggtgaat acctataccg gagtattat
//
LOCUS       DADSYM010000179         7770 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2415
            SAMN08039579-rid11723173.denovo.181, whole genome shotgun sequence.
ACCESSION   DADSYM010000179 DADSYM010000000
VERSION     DADSYM010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039579
            Sequence Read Archive: SRR6362533
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7770)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7770)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980162.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 03:39:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,484
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 4,347
            CDSs (with protein)               :: 4,347
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 43 of 85
            Pseudo Genes (incomplete)         :: 37 of 85
            Pseudo Genes (internal stop)      :: 19 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7770
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039579-rid11723173.denovo.181"
                     /strain="LGE2415"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8302 N 95.9628 W"
                     /collection_date="2015-08-24"
     gene            152..880
                     /gene="gadW"
                     /locus_tag="J1Y83_003901"
     CDS             152..880
                     /gene="gadW"
                     /locus_tag="J1Y83_003901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadW"
                     /protein_id="HBA7632004.1"
                     /translation="MAHVCSVILVRRSFDIHHEQQKISLHNESILLLDKNLADDFAFC
                     SLDTRRLDIEELTVCHYLQNIRQLPRNLGLHSKDRLLINQSPPIQLVTAIFDSFNDPR
                     VNSPILSKMLYLSCLSMFSHKKELIPLLFNSISTVSGKVERLISFDIAKRWYLRDIAE
                     RMYTSESLIKKKLQDENTCFSKILLASRMSMARRLLELRQIPLHTIAEKCGYSSTSYF
                     INTFRQYYGVTPHQFSQHSPGTFS"
     gene            complement(1243..4356)
                     /locus_tag="J1Y83_003902"
     CDS             complement(1243..4356)
                     /locus_tag="J1Y83_003902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit"
                     /protein_id="HBA7632005.1"
                     /translation="MANYFIDRPVFAWVLAIIMMLAGGLAIMNLPVAQYPQIAPPTIT
                     ISATYPGADAQTVEDSVTQVIEQNMNGLDGLMYMSSTSDAAGNASITLTFETGTSPDI
                     AQVQVQNKLQLAMPSLPEAVQQQGISVDKSSSNILMVAAFISDNGSLNQYDIADYVAS
                     NIKDPLSRTAGVGSVQLFGSEYAMRIWLDPQKLNKYNLVPSDVISQIKVQNNQISGGQ
                     LGGMPQAADQQLNASIIVQTRLQTPEEFGKILLKVQQDGSQVLLRDVAHVELGAEDYS
                     TVARYNGKPAAGIAIKLATGANALDTSRAVKEELNRLSAYFPASLKTVYPYDTTPFIK
                     ISIQEVFKTLVEAIILVFLVMYLFLQNFRATIIPTIAVPVVILGTFAILSAVGFTINT
                     LTMFGMVLAIGLLVDDAIVVVENVERVIAEDKLPPKEATHKSMGQIQRALVGIAVVLS
                     AVFMPMAFMSGATGEIYRQFSITLISSMLLSVFVAMSLTPALCATILKAAPEGGHKPN
                     ALFARFNTLFEKSTQHYTDSTRSLLRCTGRYMVVYLLICAGMAVLFLRTPTSFLPEED
                     QGVFMTTAQLPSGATMVNTTKVLQQVTDYYLTKEKNNVQSVFTVGGFGFSGQGQNNGL
                     AFISLKPWSERVGEENSVTAIIQRAMIALSSINKAVVFPFNLPAVAELGTASGFDMEL
                     LDNGNLGHEKLTQARNELLSLAAQSPNQVIGVRPNGLEDTPMFKVNVNAAKAEAMGVA
                     LSDINQTISTAFGSSYVNDFLNQGRVKKVYVQAGTPFRMLPDNINQWYVRNASGTMAP
                     LSAYSSTEWTYGSPRLERYNGIPSMEILGEAAAGKSTGDAMKFMADLVAKLPAGVGYS
                     WTGLSYQEALSSNQAPALYAISLVVVFLALAALYESWSIPFSVMLVVPLGVVGALLAT
                     DLRGLSNDVYFQVGLLTTIGLSAKNAILIVEFAVEMMQKEGKTPVEAIIEAARMRLRP
                     ILMTSLAFILGVLPLVISHGAGSGAQNAVGTGVMGGMFAATVLAIYFVPVFFVVVEHL
                     FARFKKA"
     gene            complement(4381..5538)
                     /gene="mdtE"
                     /locus_tag="J1Y83_003903"
     CDS             complement(4381..5538)
                     /gene="mdtE"
                     /locus_tag="J1Y83_003903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter subunit MdtE"
                     /protein_id="HBA7632006.1"
                     /translation="MNRRRKLLIPLLFCGAMLTACDDKSAENAAAMTPEVGVVTLSPG
                     SVNVLSELPGRTVPYEVAEIRPQVGGIIIKRNFIEGDKVNQGDSLYQIDPAPLQADLN
                     SAKGTLAKALSTASNARITFNRQASLLKTNYVSRQDYDTARTQLNEAEANVTVAKAAV
                     EQATINLQYANVTSPITGVSGKSSVTVGALVTANQADSLVTVQRLDPIYVDLTQSVQD
                     FLRMKEEVASGQIKQVQGSTPVQLNLENGKRYGQTGTLKFSDPTVDETTGSVTLRAIF
                     PNPNGDLLPGMYVTALVDEGSRQNVLLVPQEGVTHNAQGKATALILDKDDVVQLREIE
                     ASKAIGDQWVVTSGLQAGDRVIVSGLQRIRPGIKARAISSSQENASTESKQ"
     gene            5598..5927
                     /locus_tag="J1Y83_003904"
     CDS             5598..5927
                     /locus_tag="J1Y83_003904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001205317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA7632007.1"
                     /translation="MRDLQTSGIVGLSASKMGCKHSDRHGEVNDKKNVAVLPTVSASM
                     RATKGSTGGYTYQGNKVVFVSPSIITGGIEISICIPRHASHQFQLLMSLLKIRCDTQG
                     DDVAHTS"
     gene            complement(5877..6404)
                     /gene="gadE"
                     /locus_tag="J1Y83_003905"
     CDS             complement(5877..6404)
                     /gene="gadE"
                     /locus_tag="J1Y83_003905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadE"
                     /protein_id="HBA7632008.1"
                     /translation="MIFLMTKDSFLLQGFWQLKDNHEMIKINSLSEIKKVGNKPFKVI
                     IDTYHNHILDEEAIKFLEKLDAERIIVLAPYHISKLKAKAPIYFVSRKESIKNLLEIT
                     YGKHLPHKNSQLCFSHNQFKIMQLILKNKNESNITSTLNISQQTLKIQKFNIMYKLKL
                     RRMSDIVTLGITSYF"
     gene            complement(7203..7766)
                     /gene="hdeD"
                     /locus_tag="J1Y83_003906"
     CDS             complement(7203..7766)
                     /gene="hdeD"
                     /locus_tag="J1Y83_003906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-resistance protein HdeD"
                     /protein_id="HBA7632009.1"
                     /translation="MDKATILKFDLEMLKKHRRAIQFIAVLLFIVGLLCISFPFVSGD
                     ILSTVVGALLICSGIALIVGLFSNRSHNFWPVLSGFLVAVAYLLIGYFFIRAPELGIF
                     AIAAFIAGLFCVAGVIRLMSWYRQRSMKGSWLQLVIGVLDIVIAWIFLGATPMVSVTL
                     VSTLVGIELIFSAASLFSFASLFVKKQ"
BASE COUNT         2042 a         1851 c         1833 g         2044 t
ORIGIN      
        1 tattatcaat taactgttat gcaaaactac tttgtggata aaatttggtc ctaccaaata
       61 tggcagtttt tgcacacaga aacagtctgg cgtcatttca ttagtatact gacattgaaa
      121 taatcgcagt aatgaaatat aagggatagt catggctcat gtctgctcgg tgatcctcgt
      181 tcgtcgttca ttcgatattc atcatgaaca gcaaaaaata tcgttgcata acgagagtat
      241 cctgctgctg gataaaaatt tggcagacga ttttgcgttt tgttcactgg atacgcgacg
      301 gctggatatc gaagagctga cagtttgcca ttacttacaa aatattcgtc agttgccacg
      361 caatttagga ttgcatagca aagaccgttt gttaattaac cagtcacccc ccatacagct
      421 ggtgacggcg atttttgata gtttcaatga cccccgggtc aattcgccga tactgagcaa
      481 aatgctctat ctttcctgtt tatcaatgtt ttctcataag aaagaactga tccccttact
      541 tttcaatagt atcagtactg tttcaggaaa agttgaacgc cttattagct ttgatatcgc
      601 taaacgttgg tatctacgcg atatcgcaga aagaatgtat accagcgaga gtctcatcaa
      661 aaaaaagttg caggatgaaa atacctgttt cagtaaaata ttactcgcct ccaggatgtc
      721 gatggccaga cgattactcg agttacgtca aatacctctg catactattg cggaaaaatg
      781 tggctatagc agtacgtcat actttataaa tacatttaga caatattatg gtgtaacgcc
      841 acatcagttt tcgcaacatt cgccgggtac cttttcctga catatttcgc atttgaatat
      901 tggtcaggat ctcacgtctg cttcatgtga aactctttcc tgatgatttc tgccgggcta
      961 ccggctagtt ctctttcgca gtttgccagg cggtataggt attttcagga ggaaaagact
     1021 cggcatgttt gggattatta agctaacaat tcatacgatt acgggaatgt gggtcagtat
     1081 tgtgctgttc aaattgatga cgaatggctg gagtggtttc tactttcagt gctgtgtact
     1141 aagcctggta ttcctgacgg ttagctggtt gttgagtggt gaatggttag caggaaagag
     1201 taaggctgaa ccctcacgtt caaccttact ctcatttaca cgttacgctt ttttaaagcg
     1261 ggcaaagaga tgttccacta caacgaaaaa gaccggaacg aagtaaattg ccagcactgt
     1321 tgcggcaaac atcccgccca ttacgccggt acctaccgcg ttttgcgcgc cagagccggc
     1381 accatgactg ataaccagcg gcagcacgcc gagaataaag gccagagaag tcatcaggat
     1441 tgggcgtaaa cgcatacgcg ctgcttcgat gattgcttct accggcgttt tcccttcttt
     1501 ctgcatcatc tcaacggcaa attcgacaat caggatggcg ttcttggcgg aaagcccgat
     1561 ggtggtcagc aaaccaacct ggaagtaaac gtcattactt aagccgcgca gatcggtggc
     1621 cagtaatgcg ccaacgacgc ctaacggaac aaccaacatc accgagaacg gaattgacca
     1681 gctttcataa agtgcggcga gggcgaggaa caccacgacc agtgaaatgg catatagcgc
     1741 gggagcctgg tttgaggata acgcttcctg atacgacagt ccggtccatg agtagccgac
     1801 gcctgccgga agttttgcga ccaggtctgc cataaatttc atggcgtcac cggtactttt
     1861 cccggcagcc gcttcaccta aaatctccat tgacgggatg ccgttgtagc gttccagtcg
     1921 cggtgaacca taggtccatt cggtagacga ataggcagaa agcggtgcca tcgtgccaga
     1981 ggcgttgcgt acataccatt ggttgatgtt atccggcaac atacggaacg gcgtgcctgc
     2041 ctggacatac acttttttca cccgcccctg gttgaggaag tcgttcacgt agctgctgcc
     2101 gaaggcggtg gaaattgtct ggttgatatc agacagcgcc acacccatag cctcggcttt
     2161 cgcagcgttg acgttcactt tgaacatcgg cgtatcttcc aggccgttcg ggcgtacccc
     2221 gatgacctga ttcggtgatt gcgctgccag tgataacagc tcgtttcgcg cctgggtcag
     2281 cttttcgtgc cccagattgc cgttgtccag cagttccata tcaaaacctg acgcggtacc
     2341 cagttcagcc accgcgggta agttgaacgg gaagacgacg gctttattga tactgcttag
     2401 cgcaatcatt gcccgctgaa taatcgcggt aaccgagttt tcctcgccga cacgttcaga
     2461 ccacggcttg aggctgataa acgccagacc gttgttttgc ccctgaccac tgaagccaaa
     2521 gccaccaacg gtaaacaccg actgaacatt atttttctct tttgtcagat aataatccgt
     2581 cacctgttgt agcactttcg tggtgttaac catggttgca ccggaaggta actgcgcggt
     2641 ggtcataaat accccctgat cctcttctgg taagaaagag gtcggcgtac gcaggaacag
     2701 caccgccatc ccggcgcaaa tcagcaggta gaccaccatg tagcgaccgg tacaacgcaa
     2761 cagcgagcgg gtgctgtcgg tatagtgctg agttgatttt tcaaacagcg tgttgaagcg
     2821 tgcgaacagg gcgttaggtt tgtgaccgcc ctccggcgcg gctttcagaa tggtggcgca
     2881 cagggcaggg gtcaggctca ttgctacaaa tactgaaagc agcatggagg agatcagcgt
     2941 gatggagaac tggcggtaga tctccccggt cgcaccgctc ataaaggcca tcggcataaa
     3001 cactgcggaa agaacaacgg caatcccgac cagcgcacgt tggatctgcc ccatcgattt
     3061 atgcgtcgct tccttcggcg gtagcttatc ttccgcaatg acacgctcga cgttctccac
     3121 caccacgatg gcgtcatcca ccagtaaccc tatcgccagc accatcccga acatagttaa
     3181 cgtgttgatg gtgaaaccga ccgccgacaa gatcgcaaac gtcccgagaa taaccaccgg
     3241 tacggcaatc gtcgggatga ttgtggcgcg gaaattctgc aaaaacagat acatgaccag
     3301 gaagacgagg atgatagcct caaccagcgt tttgaaaact tcctgaatag aaattttgat
     3361 aaacggtgtg gtgtcgtaag gataaaccgt cttcaggctt gcagggaaat aagccgataa
     3421 acggttcagt tcctctttca ccgctcgcga ggtatccagg gcgtttgctc cggtagccag
     3481 tttgatggcg atcccggcag caggtttgcc gttatagcgc gccacggtgg aataatcttc
     3541 cgccccaagt tcgacgtggg cgacatcacg cagcagtact tgcgaaccat cttgctgaac
     3601 tttcaacagg attttgccaa attcttccgg cgtttgcaga cgcgtctgca caatgatcga
     3661 ggcgttcagt tgctggtctg ccgcctgtgg catgccaccc agttgaccac cggaaatctg
     3721 gttgttttgc accttaatct gggaaataac atcggaaggt accaggttat atttattgag
     3781 tttttgcggg tccagccaga tacgcatggc gtactcggaa ccaaagagct gtacgctacc
     3841 aacgcccgcg gtacggctta gcgggtcttt gatattagac gctacatagt ccgcgatatc
     3901 gtactggttg aggctgccgt tatcagaaat aaatgccgct accatcagga tattactgct
     3961 cgacttatcg acgctaatcc cctgctgctg caccgcttca ggtaatgaag gcatagcgag
     4021 ttgcagttta ttttgcacct gaacctgtgc gatatcaggt gatgtccccg tctcgaaggt
     4081 cagagtgatg gaggcattac ctgccgcatc gctggttgaa gacatgtaca tcaggccatc
     4141 aagcccattc atattttgct caatcacctg agtgaccgag tcttctaccg tttgcgcatc
     4201 ggcacctgga taggtagcgc tgatggtaat ggtcggtggc gcaatctgcg gatactgcgc
     4261 aaccggtaag ttcatgatcg ccagaccgcc tgcaagcatc ataataatgg caagtaccca
     4321 ggcaaaaacc gggcgatcaa taaaatagtt agccatgaaa gtccccttaa gcctgcaacg
     4381 ttattgtttc gattcggtgc tggcgttttc ctggctggag gaaattgcac gtgctttgat
     4441 acccggacga atgcgttgca aaccggaaac gatcacccga tcgccagcct gcaagccaga
     4501 ggtgacaacc cactggtcgc cgatggcttt gctggcttcg atttcgcgta gctgcacgac
     4561 atcgtcttta tccagaatga gtgccgttgc tttaccctgg gcgttgtggg tgacgccttc
     4621 ctgcggcacc agtaatacat tctggcggct accttcatcc actaatgccg tgacatacat
     4681 gccaggcagc aagtcaccat ttgggttggg gaaaatcgcc cgtaacgtca cggagcccgt
     4741 ggtttcatcc accgtcgggt cggagaattt cagcgtgccg gtctggccgt aacgtttacc
     4801 attttccaga ttgagctgta ctggcgtact gccctgaacc tgtttgattt gcccactggc
     4861 gacctcttct ttcatgcgca agaaatcctg caccgactgc gtgagatcga cataaatcgg
     4921 gtccagacgt tgtacggtaa ccagcgaatc tgcctgatta gcggtaacga gtgcgccgac
     4981 ggtcaccgac gatttcccgc tgacgcccgt aatcggcgag gtgacattcg cgtattgcag
     5041 attgatcgtc gcctgttcaa cagccgcttt ggcgacggtg acattggctt ctgcttcatt
     5101 caactgggtg cgcgcggtgt cgtaatcctg acggctaaca tagttggtct tcagcaacga
     5161 tgcctggcgg ttaaaggtga tgcgggcatt gctggcggta gagagcgctt tcgccagcgt
     5221 gcctttggcg gagttcagat cggcctgtaa aggcgcagga tcaatctgat acagtgaatc
     5281 gccctggttc actttatcgc cttcgataaa gttgcgttta atgataatac cgcccacctg
     5341 gggacgtatc tcggcaactt cataaggaac ggttctaccg ggcaattcgc tcaacacatt
     5401 gaccgaaccg ggggagagtg tgacgacacc gacctcaggc gtcatggcgg cggcgttttc
     5461 cgccgatttg tcatcgcagg cggtgagcat cgcgccgcag aataacaacg gtattaacag
     5521 ttttcttctt ctgttcattt tagtccctga aaattcttga gaaataacgg ttctttaaat
     5581 tgcgcgcgag gagagatatg agggatttac agaccagcgg catagtgggg ttgtcagcca
     5641 gtaagatggg atgcaagcac agtgatcgac atggtgaggt caacgacaaa aaaaatgttg
     5701 ccgttctgcc aacagtttct gccagcatgc gggcaacgaa gggttctact ggtggataca
     5761 cataccaggg gaataaagtc gttttcgtct ctccttcaat aatcaccggg ggtattgaaa
     5821 tcagcatctg catccctcgt catgccagcc atcaatttca gttgcttatg tccttactaa
     5881 aaataagatg tgatacccag ggtgacgatg tcgctcatac gtcttaattt cagcttgtac
     5941 ataatgttga atttctgaat ctttaatgtt tgttgcgaaa tgttgagcgt tgacgtgata
     6001 ttgctttcat ttttgttttt cagaatcagt tgcataattt tgaactgatt atgtgaaaaa
     6061 catagttgtg aattcttatg gggcaagtgt ttaccataag taatctcaag aagatttttg
     6121 atactttctt tacggctgac aaaataaata ggcgctttag cttttagttt actgatgtga
     6181 taaggtgcca aaacaataat tctctcggca tctaatttct ccaggaattt aatcgcttct
     6241 tcatcaagga tatggttgtg ataggtgtca atgatcacct tgaagggttt attgcctact
     6301 tttttgatct ctgacaggga attgattttt atcatttcgt gattatcttt caactgccaa
     6361 aagccctgta aaagaaaaga atctttcgtc atgagaaaaa tcataacttg ctccttagcc
     6421 gttatcgttt cgaacatgtc atccttttca ttatttacat ccttgtccga atcgttgttc
     6481 aatatagtaa acgcctataa gaactttgcc cttcgtgaac taatacgtct attaacacta
     6541 atcaatactt cagatttatc catcacgcac tccatatatt ggtcctaagt aagattcctg
     6601 gttgttatca gtttgtaact agtaaacaaa aggttaactt ttgcggatta ctgcccaaga
     6661 ataagttatc aataaccagt ttgtgatctc tgaagaatat tactaaagtt aaaatgtaat
     6721 ccgatttaaa tatcgagtct ccttgtttcg acttaggctg gcaattggat tgccagcttt
     6781 cttttaacgc attgaaatta cctgattaaa taactcattc atgtcttcag gtttttatat
     6841 agcgtctgga gacacggata tttatgcaat gtaatcaacg ccagttgcaa aaaaatgaac
     6901 ccggattact tatttaaccg cccatagcat caacgcttac ggaactcttt gttcactttc
     6961 ccacagcact tttaaaacat taaacctgac cacggaaaat atcagccatg ataatgtttg
     7021 aaatggctaa tttgccatgg aacgaaaaaa atcagattaa attcagtagg ttgaaataat
     7081 caacagcagg caattgtttc aatgatagtg cgtaattgaa ctgaaacact gtatagctaa
     7141 gagagtatta actggatcat agtcacttaa tgtgactatg atccaggtgg aaatagggat
     7201 aattattgct ttttaacgaa caaactggcg aagctgaaca gactggcggc gctaaatatc
     7261 agttcaattc ccaccagtgt ggaaaccagc gttaccgaca ccatcggcgt agcaccaagg
     7321 aatatccagg caatgacgat atccagcaca ccaataacga gctgtagcca actgcctttc
     7381 attgaacgct gacgatacca actcatcagg cgaataaccc ctgccacaca gaacaaaccg
     7441 gcaataaatg ccgcaatggc aaaaatgccc agctctggtg cgcggatgaa gaaatagccg
     7501 atcaataaat aggcgactgc gacgaggaaa ccggataata ccggccagaa attatgactg
     7561 cggttgctga atagcccgac aataagcgca atgcccgagc agattaataa tgcacccact
     7621 actgtgctta aaatgtcgcc agagacgaac ggaaaactga tacacagcaa cccgacgata
     7681 aacagcagca cggcaataaa ctggattgct ctgcgatgtt ttttaagcat ctccagatca
     7741 aacttcaaaa ttgttgcctt atctatatat
//
LOCUS       DADSYM010000180         7730 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2415
            SAMN08039579-rid11723173.denovo.182, whole genome shotgun sequence.
ACCESSION   DADSYM010000180 DADSYM010000000
VERSION     DADSYM010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039579
            Sequence Read Archive: SRR6362533
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7730)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7730)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980162.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 03:39:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,484
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 4,347
            CDSs (with protein)               :: 4,347
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 43 of 85
            Pseudo Genes (incomplete)         :: 37 of 85
            Pseudo Genes (internal stop)      :: 19 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7730
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039579-rid11723173.denovo.182"
                     /strain="LGE2415"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8302 N 95.9628 W"
                     /collection_date="2015-08-24"
     gene            4..993
                     /gene="ldhA"
                     /locus_tag="J1Y83_003907"
     CDS             4..993
                     /gene="ldhA"
                     /locus_tag="J1Y83_003907"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HBA7632010.1"
                     /translation="MKLAVYSTKQYDKKYLQQVNESFGFELEFFDFLLTEKTAKTANG
                     CEAVCIFVNDDGSRPVLEELKKHGVKYIALRCAGFNNVDLDAAKELGLKVVRVPAYDP
                     EAVAEHAIGMMMTLNRRIHRAYQRTRDANFSLEGLTGFTMYGKTAGVIGTGKIGVAML
                     RILKGFGMRLLAFDPYPSAAALELGVEYVDLPTLFSESDVISLHCPLTPENYHLLNEA
                     AFDQMKNGVMIVNTSRGALIDSQAAIEALKNQKIGSLGMDVYENERDLFFEDKSNDVI
                     QDDVFRRLSACHNVLFTGHQAFLTAEALTSISQTTLQNLSNLEKGETCPNELV"
     gene            1104..1526
                     /gene="hslJ"
                     /locus_tag="J1Y83_003908"
     CDS             1104..1526
                     /gene="hslJ"
                     /locus_tag="J1Y83_003908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock protein HslJ"
                     /protein_id="HBA7632011.1"
                     /translation="MKKVAALVALSLLMAGCVSSDKIAVTPEQLQHHRFVLESVNGKP
                     VTSDKNPPEISFGEKMMISGSMCNRFSGEGKLSNGELTAKGLAMTRMMCVNPQLNELD
                     NTISEMLKEGAQVDLTANQLTLATAKQTLTYKLADLMN"
     gene            complement(1523..1789)
                     /locus_tag="J1Y83_003909"
     CDS             complement(1523..1789)
                     /locus_tag="J1Y83_003909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015964583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF333 domain-containing protein"
                     /protein_id="HBA7632012.1"
                     /translation="MRAAFWVGCAALLLSACSSEPVQQATAAHVAPGLKASMSSSGEA
                     NCAMIGGSLSVARQLDGTAIGMCALPNGKRCSEQSLAAGSCGSY"
     gene            2063..5587
                     /gene="nifJ"
                     /locus_tag="J1Y83_003910"
     CDS             2063..5587
                     /gene="nifJ"
                     /locus_tag="J1Y83_003910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HBA7632013.1"
                     /translation="MITIDGNGAVASVAFRTSEVIAIYPITPSSTMAEQADAWAGNGL
                     KNVWGDTPRVVEMQSEAGAIATVHGALQTGALSTSFTSSQGLLLMIPTLYKLAGELTP
                     FVLHVAARTVATHALSIFGDHSDVMAVRQTGCAMLCAANVQEAQDFALISHIATLKSR
                     VPFIHFFDGFRTSHEINKIVPLADDTILELMPQAEIDAHRARALNPEHPVIRGTSANP
                     DTYFQSREATNPWYNAVYDHVEQAMNDFAAATGRQYQPFEYYGHPQAERVIILMGSAI
                     GTSEEVVDELLTRGEKVGVLKVRLYRPFSAKHLLQALPGSVRNVAVLDRTKEPGAQAE
                     PLYLDVMTALAEAFNNGERETLPRVIGGRYGLSSKEFGPDCVLAVFAELNAAKPKARF
                     TVGIYDDVTNLSLPLPENTLPNSAKLEALFYGLGSDGSVSATKNNIKIIGNSTPWYAQ
                     GYFVYDSKKAGGLTVSHLRVSEQPIRSAYLISQADFVGCHQLQFIDKYQMAERLKPGG
                     IFLLNTPYSAAEVWSRLPQEVQAVLNQKKARFYVINAAKIARECGLAARINTVMQMAF
                     FHLTQILPGDSALAELQGAIAKSYSSKGQDLVERNWQALALARESVEEVPLQPVNPHS
                     ANRPPVVSDAAPDFVKTVTAAMLAGLGDALPVSALPPDGTWPMGTTRWEKRNIAEEIP
                     IWKEELCTQCNHCVAACPHSAIRAKVVPPEAMENAPASLHSLDVKSRDMRGQKYVLQV
                     APEDCTGCNLCVEVCPAKDRQNPEIKAINMMSRLEHVEEEKINYDFFLNLPEIDRSKL
                     ERIDIRTSQLITPLFEYSGACSGCGETPYIKLLTQLYGDRMLIANATGCSSIYGGNLP
                     STPYTTDANGRGPAWANSLFEDNAEFGLGFRLTVDQHRVRVLRLLDQFADKIPAELLT
                     ALKSDATPEVRREQVAALRQQLNDVAEAHELLRDADALVEKSIWLIGGDGWAYDIGFG
                     GLDHVLSLTENVNILVLDTQCYSNTGGQASKATPLGAVTKFGEHGKRKARKDLGVSMM
                     MYGHVYVAQISLGAQLNQTVKAIQEAEAYPGPSLIIAYSPCEEHGYDLALSHDQMRQL
                     TATGFWPLYRFDPRRADEGKLPLALDSRPPSEALEETLLHEQRFRRLNSQQPEVAEQL
                     WKDAAADLQKRYDFLAQMAGKAEKSNTD"
     gene            5953..7086
                     /gene="ompN"
                     /locus_tag="J1Y83_003911"
     CDS             5953..7086
                     /gene="ompN"
                     /locus_tag="J1Y83_003911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpN"
                     /protein_id="HBA7632014.1"
                     /translation="MKSKVLALLIPALLAAGAAHAAEVYNKDGNKLDLYGKVDGLHYF
                     SDNSAKDGDQSYARLGFKGETQINDQLTGYGQWEYNIQANNTESSTNQSWTRLAFAGL
                     KFADYGSFDYGRNYGVMYDIEGWTDMLPEFGGDSYTNADNFMTGRANGVATYRNTDFF
                     GLVNGLNFAVQYQGNNEGASNGQEGTNNGRDVRHENGDGWGLSTTYDLGMGFSAGAAY
                     TSSDRTNDQVNHTAAGGDKADAWTAGLKYDANNIYLATMYSETRNMTPFGDSDYAVAN
                     KTQNFEVTAQYQFDFGLRPAVSFLMSKGRDLHAAGGADNPAGVDDKDLVKYADVGATY
                     YFNKNMSTYVDYKINLLDEDDSFYAANGISTDDIVALGLVYQF"
     gene            7227..7661
                     /gene="uspF"
                     /locus_tag="J1Y83_003912"
     CDS             7227..7661
                     /gene="uspF"
                     /locus_tag="J1Y83_003912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310024.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspF"
                     /protein_id="HBA7632015.1"
                     /translation="MNRTILVPIDISDSELTQRVISHVEAEAKIDDAEVHFLTVIPSL
                     PYYASLGLAYSAELPAMDDLKAEAKSQLEEIIKKFKLPTDRVHVHVEEGSPKDRILEL
                     AKKIPAHMIIIASHRPDITTYLLGSNAAAVVRHAECSVLVVR"
BASE COUNT         1980 a         1927 c         1925 g         1898 t
ORIGIN      
        1 cttatgaaac tcgccgttta tagcacaaaa cagtacgaca agaagtacct gcaacaggtg
       61 aacgagtcct ttggctttga gctggaattt tttgactttc tgctgacgga aaaaaccgct
      121 aaaactgcca atggctgcga agcggtatgt attttcgtaa acgatgacgg cagccgcccg
      181 gtgctggaag agctgaaaaa gcacggcgtt aaatatatcg ccttgcgctg tgccggtttc
      241 aataacgtcg accttgacgc ggcaaaagaa ctggggctca aagtagtccg tgttccagcc
      301 tatgatccag aggccgttgc tgaacacgcc atcggtatga tgatgacgct gaaccgccgt
      361 attcaccgcg cgtatcagcg tacccgtgac gctaacttct ctctggaagg tctgaccggc
      421 tttactatgt atggcaaaac ggcaggcgtt atcggtaccg gtaaaatcgg tgtggcgatg
      481 ctgcgcattc tgaaaggttt tggtatgcgt ctgctggcgt tcgatccgta tccaagtgcg
      541 gcggcgctgg aactcggtgt ggagtatgtt gatctgccaa ccctgttctc tgaatcagac
      601 gttatctctc tgcactgccc gctgacaccg gaaaactacc atctgttgaa cgaagccgcc
      661 ttcgatcaga tgaaaaatgg cgtgatgatc gtcaatacca gtcgcggtgc attgattgat
      721 tctcaggcgg caattgaagc gctgaaaaat cagaaaattg gttcgttggg tatggacgtg
      781 tatgagaacg aacgcgatct gttctttgaa gataaatcca acgacgtgat ccaggatgac
      841 gtattccgtc gcttgtctgc ctgccacaac gtgctgttta ccgggcacca ggcattcctg
      901 acagcagaag ctctgaccag tatttctcag actacgctgc aaaacttaag caatctggaa
      961 aaaggcgaaa cctgcccgaa cgaactggtt taatcttgcc gctcccctgc attccagggg
     1021 agcttattca gataatcccc aaagaccttt catcctctat tcttaaaata gtcctgaatc
     1081 agaaacagta attgagaacc acaatgaaga aagtagccgc gcttgttgcg ctaagcctgc
     1141 tgatggcggg atgtgtaagt agtgacaaaa ttgctgtaac gccagaacag ctacagcatc
     1201 atcgctttgt gctggaaagc gtaaacggta agcccgtgac cagcgataaa aatccgccag
     1261 aaatcagctt tggtgaaaag atgatgattt ccggcagcat gtgtaaccgc tttagcggtg
     1321 aaggcaaact gtctaatggt gaactgaccg ccaaagggct ggcaatgacc cgtatgatgt
     1381 gcgttaaccc gcagcttaat gaactcgata acaccattag cgaaatgctg aaagaaggtg
     1441 cacaagtgga tctgaccgcg aaccagttaa cgctggcgac cgcgaaacag acattaactt
     1501 ataagctggc ggatttaatg aattaatagc tgccacagct cccggcggca agtgactgtt
     1561 cgctacagcg tttgccgttg ggtaatgcac acatcccaat cgccgtacca tccagttggc
     1621 gggcaacaga aagcgaaccg ccgatcattg cacaatttgc ttctccacta ctggacatcg
     1681 acgcttttaa acctggcgct acgtgcgccg cagtcgcctg ctgaacaggt tcactactac
     1741 acgccgacaa caataaagcg gcacacccta cccaaaacgc tgctcgcatc ttttcttcct
     1801 ctgatcttca agccaaacga caccgccata aataataggc agcacagagg gcgacgtcga
     1861 gagctgtcct gcgcgttgcc ccgcattttt acttttttat ggctattttt ttgccctctg
     1921 tttgatcaaa acattcatta cgctgatgtg ggggacacaa aagcgaaaat gcagaagaaa
     1981 gccatttgct aaaattgaac gattactact gggcgcgcag caatttcgtg cgcccctcat
     2041 ttgcgcaatg taagggtgtc atatgattac tattgacggt aatggcgcgg ttgcttcggt
     2101 ggcgtttcgc accagtgaag ttatcgccat ctaccctatt acccccagtt ccacgatggc
     2161 agaacaggct gatgcatggg ccggaaacgg cttgaaaaac gtttggggag acacaccacg
     2221 cgtggttgaa atgcagtcgg aagcgggtgc tatcgctacc gtgcatggtg ctttgcaaac
     2281 tggtgccctc tcaacatcgt ttacgtcatc gcagggtttg ctgctgatga tcccgacgct
     2341 gtacaaactg gcaggcgaac tcacaccgtt tgtcctgcat gttgcggcac gtaccgttgc
     2401 cacacatgca ctctctattt ttggcgatca ttccgacgtt atggcggtgc gccaaacggg
     2461 ttgcgcgatg ttgtgtgcag caaacgtcca ggaagcgcaa gactttgctc tcatttcgca
     2521 cattgcgaca ctgaaaagcc gcgtgccatt tattcatttc tttgatggtt tccgcacatc
     2581 ccacgaaatc aataaaattg tcccgctggc cgatgacacg attcttgaac tgatgccgca
     2641 ggcagaaatt gatgctcatc gcgcccgggc actcaacccg gaacatccgg tgatccgcgg
     2701 tacgtccgcc aatcctgaca cttatttcca gtctcgcgaa gccaccaacc catggtacaa
     2761 cgcggtctat gaccatgttg aacaggcgat gaatgatttc gctgccgcga caggtcgtca
     2821 gtatcagccg tttgagtatt acgggcatcc gcaagcggaa cgggtgatta tcctgatggg
     2881 ctctgccatt ggcaccagtg aagaagtggt tgatgaattg ctgacccgtg gcgaaaaagt
     2941 cggcgtattg aaagttcgct tgtaccgccc cttctccgcc aaacacttgc tgcaagctct
     3001 gccgggatcc gtgcgcaacg tggcggtact ggacagaacc aaagagcctg gtgcccaggc
     3061 agaaccgctc tatctggatg tgatgaccgc actggcagaa gcctttaata atggcgagcg
     3121 tgaaaccctg ccccgtgtca ttggtgggcg ctatggtctt tcatccaaag aatttggccc
     3181 ggactgtgta ctggcggtat ttgccgagct caacgcggct aaaccgaaag cgcgctttac
     3241 ggttggtatt tacgatgatg taaccaatct gtcactgccg ttgccggaaa acaccctacc
     3301 aaactcggcg aaactggaag ccttgtttta tggccttggt agtgatggca gcgtttccgc
     3361 gaccaaaaac aatatcaaga ttatcggaaa ttccacgccg tggtacgcac agggctattt
     3421 tgtttacgac tccaaaaagg cgggcggcct gacggtttct caccttcgcg tgagcgaaca
     3481 gccgattcgt tccgcttatc tcatttccca ggctgatttt gttggctgcc accagttgca
     3541 gtttatcgat aaatatcaga tggctgagcg tttaaaacct ggcggcattt tcctgctcaa
     3601 cacgccgtac agcgcagctg aagtgtggtc gcgcttgccg caggaagttc aggccgtgct
     3661 aaaccagaaa aaagcgcgct tctatgtgat taacgcggcg aaaatcgccc gtgaatgtgg
     3721 cctggcggcc cgtattaata ccgtcatgca gatggctttt ttccatctga cgcaaattct
     3781 gcctggcgat agtgccctcg cagaattgca gggtgcgatt gccaaaagtt acagtagcaa
     3841 aggccaggat ctggtggaac gcaactggca ggctctggcg ctggcgcgtg aatccgtaga
     3901 agaagttcca ctgcagccag ttaatccgca tagcgccaat cgaccgcccg tggtttccga
     3961 tgccgctcct gatttcgtga aaaccgtaac cgctgcgatg ctcgccgggc ttggcgacgc
     4021 cctccccgtt tcagcgctgc cgccagacgg cacctggccg atgggcacca cgcgctggga
     4081 aaaacgcaat atcgccgaag agatccccat ctggaaagag gaactctgta cccaatgtaa
     4141 ccactgtgtc gccgcttgcc cacactcagc tattcgcgca aaagtggtac cgcctgaagc
     4201 gatggaaaac gcccctgcca gcctgcattc gctggatgtg aaatcgcgtg atatgcgcgg
     4261 gcagaaatat gtcttgcagg tggctccgga agattgcacc ggttgtaacc tgtgcgtcga
     4321 agtttgcccg gcgaaagacc gtcagaatcc agagattaaa gccatcaata tgatgtcgcg
     4381 cctggaacat gtcgaagaag agaaaatcaa ttacgatttc ttcctcaacc tgccggaaat
     4441 cgaccgtagc aaactggaac gtattgatat tcgtacatcg cagctgatta caccgctgtt
     4501 tgaatattca ggtgcttgct ccggttgtgg cgagacgccg tatattaaat tgctgactca
     4561 gctctatggc gaccggatgt tgatcgctaa tgccactggc tgttcttcaa tatacggcgg
     4621 taacctgcct tcaacaccat ataccaccga tgccaatggt cgtggaccgg catgggcgaa
     4681 ctcgctgttt gaagataacg ccgaatttgg ccttggtttc cgcctgacgg tcgatcaaca
     4741 ccgtgtccgc gtgctgcgtc tgctcgatca atttgccgat aaaatcccgg cggaattact
     4801 gactgcgttg aaatcagacg ccacgccaga ggttcgtcgt gaacaggttg cagctttacg
     4861 ccagcaactc aacgatgttg ccgaagcaca tgaactgctg cgtgacgcag atgcactggt
     4921 ggaaaaatca atctggctga ttggtggcga tggctgggct tacgatatcg gctttggcgg
     4981 tctggatcat gtattgagtt tgacggaaaa cgtcaacatt ctggtgctgg atacgcaatg
     5041 ctattccaac accggtggtc aggcatcgaa agcgacaccg ctgggtgcag taactaagtt
     5101 tggcgagcat ggcaaacgta aagcgcgtaa agatcttggc gtcagtatga tgatgtacgg
     5161 tcatgtttat gtggcgcaga tttctctcgg cgcgcagctg aaccagacgg tgaaagcgat
     5221 tcaggaagcg gaagcgtatc cggggccatc gctgatcatc gcttatagcc cgtgtgaaga
     5281 gcatggttac gatctggcac tcagccacga tcagatgcgc caactcactg ctaccggctt
     5341 ctggccgtta tatcgcttcg atcctcgtcg tgccgatgaa ggcaaactgc cgctggcgct
     5401 ggattcacgc ccgccgtcag aagcactgga agaaacgtta cttcacgagc agcgtttccg
     5461 tcgtctgaat tcgcagcagc cagaagtggc agaacagtta tggaaagatg ctgcagctga
     5521 tttgcaaaag cgctatgact tcctggcaca aatggccgga aaagcggaaa aaagcaacac
     5581 cgattaaatg ctctggataa ggatcatcca attctaaaaa aaagcccgga cgactgttcg
     5641 ggcttgtctt cttttgtgtt ggaaaatcag tagcaatgca atggcccaac agaaaataaa
     5701 atctgtgaca ataaaggcat ataacccgcg cagaataacg tatagaaaat taaaagtata
     5761 atttttattt tgtatgtgtt atctccaata cattcaccag acttattatc atttctgaag
     5821 aattatttaa ttaaggtttt acttaaggcg taacaaatga tatttatcgg ctaactgaac
     5881 ttctccttta ggatgttttc actccccaaa atggggatga aaggcaaata aaataactaa
     5941 aggatttatt caatgaaaag caaagtactg gcactcttaa ttcctgccct gctcgctgca
     6001 ggtgctgcac atgcagccga agtttataat aaagacggca acaaattaga tctgtatggc
     6061 aaagttgatg gcctgcatta tttttctgat aattcagcga aagatggcga ccagagctat
     6121 gctcgtctgg gttttaaagg cgaaacccaa attaacgatc aactcactgg ctacggtcaa
     6181 tgggaataca atattcaggc aaacaacacc gaatcttcaa caaaccagtc atggacccgt
     6241 ctggcatttg ccggactgaa atttgcagat tacggttctt tcgattacgg acgtaattat
     6301 ggcgtaatgt acgacatcga aggctggact gatatgctac ctgaatttgg cggtgactct
     6361 tataccaatg cagacaactt tatgactggt cgtgccaatg gcgtcgcgac ttatcgtaat
     6421 actgatttct tcggtctggt aaatggtctg aacttcgcgg tgcagtatca aggtaacaac
     6481 gaaggagcca gtaatggtca ggaaggcacc aacaacggac gtgatgttcg ccatgaaaac
     6541 ggtgacggct ggggtctttc cacaacatat gatttaggca tgggctttag cgctggtgcg
     6601 gcatacacct cttctgaccg caccaatgac caggttaacc atactgcggc gggtggtgat
     6661 aaagcagacg cgtggactgc tgggctaaaa tacgatgcta acaatattta cctggcaacc
     6721 atgtattcag aaacgcgtaa catgaccccg tttggcgaca gcgattatgc agttgcgaac
     6781 aaaacccaga actttgaagt tactgcacag taccagtttg attttggtct gcgtccggca
     6841 gtctctttcc tgatgtctaa aggccgtgac ctgcacgctg ccggtggtgc agacaaccca
     6901 gcaggtgttg atgataaaga tctggttaaa tacgccgatg ttggcgcgac ttactatttc
     6961 aataaaaaca tgtccaccta tgttgactat aaaatcaacc tgttggatga agatgacagt
     7021 ttctacgctg ccaacggcat ctctaccgat gatattgtcg ctttaggtct ggtttatcag
     7081 ttctaaatcc ccctgcccgc tgttttggcg ggctttttct gcttattctt cctcctttga
     7141 tctaaattaa aaatgtgaac tccgtcatta cacaaaaagt gtcatctggc gttacacttt
     7201 atgcggatac taaaacagga ggttttatga acagaacgat tcttgtccct atcgatattt
     7261 ccgattcaga attaactcaa cgcgtgatta gccacgttga ggcagaggca aagattgatg
     7321 atgcagaggt tcatttcctg acggtaatac cttcgctgcc ctactatgcc tctctgggtt
     7381 tagcctattc cgcagaatta ccggcaatgg atgacctgaa agcggaagcc aaatcgcaac
     7441 tggaagagat cattaaaaaa tttaaactgc caaccgacag agtgcatgtc catgttgaag
     7501 aaggctcgcc caaagaccgc attctggaat tggcgaagaa gatccccgct catatgatca
     7561 tcattgcttc ccatcgaccg gatatcacca cttatctgct cggttctaac gccgcagctg
     7621 tagtgcgtca cgcagagtgc tccgtgctgg ttgtgcgctg acactaacgc ccgcacattg
     7681 ctgcgggctt tttgattcat ttcgcaaatg tgctgacatt ttcccctcta
//
LOCUS       DADSYM010000181         7662 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2415
            SAMN08039579-rid11723173.denovo.183, whole genome shotgun sequence.
ACCESSION   DADSYM010000181 DADSYM010000000
VERSION     DADSYM010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039579
            Sequence Read Archive: SRR6362533
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7662)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7662)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980162.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 03:39:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,484
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 4,347
            CDSs (with protein)               :: 4,347
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 43 of 85
            Pseudo Genes (incomplete)         :: 37 of 85
            Pseudo Genes (internal stop)      :: 19 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7662
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039579-rid11723173.denovo.183"
                     /strain="LGE2415"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8302 N 95.9628 W"
                     /collection_date="2015-08-24"
     gene            379..1662
                     /locus_tag="J1Y83_003913"
     CDS             379..1662
                     /locus_tag="J1Y83_003913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020232117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MHS family MFS transporter"
                     /protein_id="HBA7632016.1"
                     /translation="MDFQLYSLGAALVFHEIFFPESSTAMALILAMGTYGAGYVARIV
                     GAFIFGKMGDRIGRKKVLFITITMMGICTTLIGVLPTYAQIGVFAPILLVTLRIIQGL
                     GAGAEISGAGTMLAEYAPKGKRGIISSFVAMGTNCGTLSATAIWAFMFFILSKEELLA
                     WGWRIPFLASVVVMVFAIWLRMNLKESPVFEKVNDSNQPTAKPAPAGSMFQSKSFWLA
                     TGLRFGQAGNSGLIQTFLAGYLVQTLLFNKAIPTDALMISSILGFMTIPFLGWLSDKI
                     GRRIPYIIMNTSAIVLAWPMLSIIVDKSYAPSTIMVALIVIHNCAVLGLFALENITMA
                     EMFGCKNRFTRMAISKEIGGLIASGFGPILAGIFCTMTESWYPIAIMIMAYSVIGLIS
                     ALKMPEVKDRDLSALEDAAEDQPHVVRAAQPSRSL"
     gene            1751..3211
                     /locus_tag="J1Y83_003914"
     CDS             1751..3211
                     /locus_tag="J1Y83_003914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HBA7632017.1"
                     /translation="MGNNLLSAKATLPVYDRNNLAPRIVHLGFGAFHRAHQGVYADIL
                     ATEHFSDWGYYEVNLIGGEQQIADLQQQDNLYTVAEMSADAWTARVVGVVKKALHVQI
                     DGLETVLAAMCEPQIAIVSLTITEKGYFHSPATGQLMLDHPMVVADLQNPHQPKTATG
                     VIVEALARRKAAGLPAFTVMSCDNMPENGHVMRDVVTSYAQAVDVKLAQWIEENVTFP
                     STMVDRIVPAVTEDTLAKIEQLTGVRDPAGVACEPFRQWVIEDNFVAGRPEWEKAGAE
                     LVSDVLPYEEMKLRTLNGSHSFLAYLGYLAGYQHINDCMEDEHYRHAAYALMLQEQAP
                     TLKVQGVDLQDYANRLIERYSNPALRHRTWQIAMDGSQKLPQRMLDSVRWHLAHDSKF
                     DLLALGVAGWMRYVGGVDEQGNPIEISDPLLPVIQKAVQSSAEGTARVQSLLAIKAIF
                     GDDLPGNSLFTTKVTEAYLSLLAHGAKATVAKYSVK"
     gene            complement(3247..3450)
                     /gene="ydfZ"
                     /locus_tag="J1Y83_003915"
     CDS             complement(3247..3450)
                     /gene="ydfZ"
                     /locus_tag="J1Y83_003915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative selenium delivery protein YdfZ"
                     /protein_id="HBA7632018.1"
                     /translation="MTTYDRNRNAITTGSRVMVSGTGHTGKILSIDTEGLTAEQIRRG
                     KTVVVEGCEEKLAPLDLIRLGMN"
     gene            complement(3628..4314)
                     /locus_tag="J1Y83_003916"
     CDS             complement(3628..4314)
                     /locus_tag="J1Y83_003916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBA7632019.1"
                     /translation="MTVETQLNPTQPVNQQIYRILRRDIVHCLIAPGTPLSEKEVSVR
                     FNVSRQPVREAFIKLAENGLIQIRPQRGSYVNKISMAQVRNGSFIRQAIECAVARRAA
                     SMITESQCYQLEQNLHQQRIAIERKQLDDFFELDDNFHQLLTQIADCQLAWDTIENLK
                     ATVDRVRYMSFDHVSPPEMLLRQHLDIFSALQKRDGDAVERAMTQHLQEISESVRQIR
                     QENSDWFSEE"
     gene            complement(4403..5149)
                     /gene="ydfG"
                     /locus_tag="J1Y83_003917"
     CDS             complement(4403..5149)
                     /gene="ydfG"
                     /locus_tag="J1Y83_003917"
                     /EC_number="1.1.1.298"
                     /EC_number="1.1.1.381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional NADP-dependent 3-hydroxy acid
                     dehydrogenase/3-hydroxypropionate dehydrogenase YdfG"
                     /protein_id="HBA7632020.1"
                     /translation="MIVLVTGATAGFGECITRRFIQQGHKVIATGRRQERLQELKDEL
                     GDNLYIAQLDVRNRAAIEEMLASLPAEWCNIDILVNNAGLALGMEPAHKASVEDWETM
                     IDTNNKGLVYMTRAVLPGMVERNHGHIINIGSTAGSWPYAGGNVYGATKAFVRQFSLN
                     LRTDLHGTAVRVTDIEPGLVGGTEFSNVRFKGDDGKAEKTYQNTVALTPEDVSEAVWW
                     VSTLPAHVNINTLEMMPVTQSYAGLNVHRQ"
     gene            5286..7331
                     /gene="dcp"
                     /locus_tag="J1Y83_003918"
     CDS             5286..7331
                     /gene="dcp"
                     /locus_tag="J1Y83_003918"
                     /EC_number="3.4.15.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-dipeptidase Dcp"
                     /protein_id="HBA7632021.1"
                     /translation="MTTMNPFLVQSTLPYLAPHFDQIANHHYRPAFDEGIQQKRAEIA
                     AIALNPQTPDFNNTILALEQSGELLTRVTSVFFAMTAAHTNDELQRLDEQFSAELAEL
                     ANDIYLNGELFARVDAVWQRRESLGLDSESIRLVEVIHQRFVLAGAKLEQADKAKLKV
                     LNTEAATLTSQFNQRLLAANKSGGLVVNDIAQLAGMSEQEIALAAEAAREKGLDNNWL
                     IPLLNTTQQPALAELRDRATREKLFTAGWTRAEKNDANDTRTIIQRLVEIRAQQAKLL
                     GFPHYAAWKIADQMAKTPEAALNFMREIVPAARQRASDELASIQAIIDKQQGGFSAQP
                     WDWAFYAEQVRREKFDLDEAQLKPYFELNTVLNEGVFWTANQLFGIKFVERFDIPVYH
                     PDVRVWEIFDHNGVGLALFYGDFFARDSKSGGAWMGNFVEQSTLNETHPVIYNVCNYQ
                     KPAAGEPALLLWDDVITLFHEFGHTLHGLFARQRYATLSGTNTPRDFVEFPSQINEHW
                     ATHPQVFARYARHYQSRAAMPDELQQKMRNASLFNKGYEMSELLSAALLDMRWHCLEE
                     NEAMQDVDDFELRALVAENMDLPAIPPRYRSSYFAHIFGGGYAAGYYAYLWTQMLADD
                     GYQWFVEQGGLTRENGRRFREAILSRGNSEDLERLYRQWRGKAPQIMPMLQHRGLNV"
     gene            7423..7584
                     /locus_tag="J1Y83_003919"
     CDS             7423..7584
                     /locus_tag="J1Y83_003919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA7632022.1"
                     /translation="MRMPFRSRQQNYFFCIPFRYNNFQIQPDQMSNISGSLDKKSAPT
                     VVRVTHLQK"
BASE COUNT         1940 a         1761 c         1956 g         2005 t
ORIGIN      
        1 tttatctgaa gaagaaaaat atcaaggtga tatagcctat atgcctttga tgcggaggaa
       61 tgaatgtgat gggagtgatg tatctgaata gttgaaaaac cgcagacacg ccttatgcaa
      121 gaacgtgctg cggttggctg gtaaattttt cgatagtgcg agtattgaat gatttccagc
      181 cgttattgat tttacgcgta aatccatgaa aaaactactt atctgttggg gagtcttttt
      241 ggggcatata tgggacagaa ataggcccca gatagacact gagatcgaac ttaggatgct
      301 ttttaaaaaa tgcaactatc tgaaaaaacc tagaaaacgc caaggaaacc acaggatggg
      361 aaaaaacacc tgtgaattat ggatttccag ttatattcgc tcggcgcagc gttagtgttt
      421 catgaaatat tttttcctga atcatcaacg gcaatggcgt taattctggc aatgggaacc
      481 tatggtgcag gttatgtggc acgtattgtc ggagcattta ttttcggcaa aatgggcgac
      541 agaatagggc gtaaaaaagt gctctttatt accatcacca tgatggggat ctgtaccacc
      601 ttaattggtg tgttaccgac ctatgcacag attggtgttt ttgcccccat cttgctggtg
      661 acgctgcgta ttattcaggg gttgggtgca ggtgcggaaa tttccggtgc cggtacgatg
      721 ctggcggaat atgcgccaaa aggtaagcgc ggaattatct cctcatttgt tgctatgggg
      781 actaactgcg gaaccttgag cgcaacggca atctgggcct ttatgttctt cattctcagt
      841 aaagaggaac tgctggcgtg gggatggcgt atacctttcc tggcgagcgt tgtcgtgatg
      901 gtctttgcta tctggttgcg tatgaatctg aaagaaagcc cggtctttga gaaggttaac
      961 gacagcaacc aaccgacagc aaaacctgca cctgctggta gcatgttcca gagcaaatcc
     1021 ttctggctgg caacagggct gcgttttggt caggctggta actcaggttt aattcagact
     1081 ttccttgcag gctatttagt gcagacgtta ttgtttaaca aagcaattcc aacagatgca
     1141 ttgatgatca gttcgattct cggctttatg accattccgt tccttggttg gttatccgat
     1201 aaaataggtc gccggatccc gtatattatt atgaatacct ctgcgattgt gctggcgtgg
     1261 ccaatgcttt ctatcatcgt agataaaagc tatgccccga gcaccattat ggttgcactg
     1321 attgtgattc ataactgtgc ggtgctggga ttatttgctc tggaaaacat taccatggca
     1381 gaaatgttcg gctgtaaaaa ccgctttacc cggatggcta tttctaaaga aattggtggt
     1441 cttatcgctt ccggttttgg tcctatcctg gcggggattt tctgcaccat gacggaatcc
     1501 tggtatccga tcgccattat gatcatggca tattctgtga ttggtttaat ctctgcgctg
     1561 aaaatgccag aagtgaaaga ccgtgattta agtgcgctgg aagacgccgc agaagatcaa
     1621 ccacatgttg taagagctgc gcaaccgtcc agaagtctgt aaatcctcaa tcccttctct
     1681 taccggagag gggattttta ttcatataac aaaacatgta gcttgccata tttatattta
     1741 aggaattgtt atgggaaata atttgttatc agcaaaagcg acgctccctg tttatgatcg
     1801 taataacctg gccccaagaa ttgttcattt aggctttggt gcatttcacc gtgcgcatca
     1861 gggtgtgtat gccgatattc ttgctacgga acatttcagt gactggggat attatgaggt
     1921 caatttaatc ggcggcgaac agcaaattgc cgatttacaa cagcaagata atctttatac
     1981 cgttgcggaa atgtcggccg atgcgtggac ggctcgcgtc gttggcgtcg ttaaaaaagc
     2041 cttgcacgta cagattgatg gcttagaaac cgtgttggct gcgatgtgtg aaccgcaaat
     2101 cgcgattgtc tctctgacaa tcaccgaaaa agggtatttc cactctccgg cgaccggaca
     2161 gttaatgctc gatcacccga tggtcgttgc cgacttacaa aatccccacc agccgaaaac
     2221 tgcaacaggg gtgattgtcg aggcgctggc tcgccgtaaa gcggcaggac ttcccgcatt
     2281 taccgtcatg tcatgtgaca acatgccaga aaacggtcat gttatgcgtg acgttgtcac
     2341 ttcctacgcg caagctgttg atgtaaaact ggcacaatgg atcgaagaaa acgtgacttt
     2401 cccatcaaca atggtggacc gtattgtgcc cgcagtgaca gaggatacgc tggcgaaaat
     2461 cgaacaactt accggtgtgc gcgatcctgc tggcgttgcc tgtgaacctt tccgccagtg
     2521 ggtaatagaa gataactttg ttgccggacg tccggaatgg gaaaaagcgg gagccgaact
     2581 ggttagtgat gtgctgcctt atgaagagat gaagttgcgc acgctcaatg gcagtcattc
     2641 attcctggcg tatctggggt atcttgccgg atatcagcac attaatgact gcatggaaga
     2701 tgaacattat cgtcatgcag cgtatgcctt aatgttgcag gaacaagcgc cgacgctgaa
     2761 agtgcagggc gttgatttgc aagattacgc taaccgatta attgaacgct atagcaaccc
     2821 ggcgttacgt catcgaacct ggcagattgc gatggatggc agccagaaat tgccacagcg
     2881 gatgttggat tctgttcgct ggcatctggc gcatgacagc aagttcgatc tgctggcgct
     2941 gggcgtcgcg ggttggatgc gttatgtcgg tggtgttgat gaacagggaa atccgataga
     3001 aatcagtgac ccactgttac ctgttattca gaaggcagta caaagtagtg ctgaagggac
     3061 agcgcgcgtc cagtcattgc tggcaattaa ggcgatcttt ggtgatgatt taccaggcaa
     3121 tagtttgttt acgacaaaag taacggaagc gtacttgtct ttattagcgc atggcgcgaa
     3181 agcgaccgtg gcgaaatatt ccgtgaagta aaaaaagcaa tgccgccatc ggcggcattc
     3241 acacgcttag ttcatgccga gacgaatcag gtccagtggt gccagtttct cttcacaacc
     3301 ttcaacaact acggtttttc cgcggcggat ttgctcagcg gtcagacctt cagtatcaat
     3361 cgacaggatc ttgccagtgt gaccggtgcc gctaaccata acacggctgc cagtggtgat
     3421 tgcgttacgg ttacgatcgt aggtcgtcat aggtgttttc tcctttctga tttacagtcg
     3481 aagcccacgt aacgggcgca atataaatac tcctgtcgag tgaaatattt tttgtttttg
     3541 atcaagctca catctttttt gatgcatgca tgagccataa ggcggggcga atagactctc
     3601 accccggatg ggatgagagg aaatgaatta ctcttcgcta aaccagtcgc tgttttcctg
     3661 gcggatctgg cgcacagatt cgctgatttc ctgcaaatgt tgcgtcattg cacgttctac
     3721 cgcatcgcca tcacgttttt gcagggcaga gaaaatatca agatgctggc gtaacagcat
     3781 ttctggtgga gaaacgtggt cgaaactcat atagcgcacg cgatcaacgg tcgctttcag
     3841 gttctcaatg gtatcccacg ccagttgaca gtcggcaatc tgcgtcagga gttgatggaa
     3901 gttgtcatca agttcaaaaa aatcatccag ttgcttgcgc tcaatggcaa tgcgttgctg
     3961 gtgaagattt tgttccagtt ggtagcactg gctttcggta atcatgctcg ccgcccgacg
     4021 cgccaccgcg cactcaatgg cctgacggat aaaactgccg ttgcgcacct gggccatgga
     4081 aattttgttg acgtagctgc cacgctgcgg acgaatttga atcagaccgt tttccgccag
     4141 tttaataaag gcttcacgaa ccggctggcg tgacacattg aaacgaacag aaacttcttt
     4201 ttccgacaac ggtgtgcctg gtgcaatcag gcaatggaca atgtcgcggc gaagaatacg
     4261 ataaatctgc tgattgacag gctgtgtggg attaagttgc gtttcgacgg tcattcgttc
     4321 ttgcttgtga gtgagttaac tgcatgagtc taccactttt ttgtgacaag caataacccg
     4381 gcagttacgc cgggtataaa aattactgac ggtggacatt cagtccggca tagctttggg
     4441 taaccggcat catttccagg gtattgatat tgacgtgagc aggcagcgtt gacacccacc
     4501 agacggcttc gctgacatct tctggcgtca atgcaacggt attttgatag gttttttccg
     4561 ctttaccgtc atcgccttta aagcggacat tggaaaactc ggtgccaccc accagacccg
     4621 gttcgatgtc ggtgacgcgc accgccgtac catgcaggtc cgtacgcaga ttcaggctaa
     4681 actgacgaac aaacgctttc gtcgcaccgt aaacgttacc accggcatac ggccagctgc
     4741 ctgccgttga gccaatgtta ataatatgac catgattacg ttcaaccata cccggtaaga
     4801 cggcgcgcgt catatatacc aggcctttgt tgttggtatc aatcatcgtt tcccagtctt
     4861 caacgctggc tttatgcgca ggctccatgc ccaacgccag accggcatta tttaccagga
     4921 tatcaatatt gcaccactcg gcaggaagcg atgccagcat ctcttcaata gcggcgcggt
     4981 tgcgaacgtc cagttgggcg atatacagat tatctcccag ttcgtctttt aactcctgta
     5041 accgctcctg gcgacggcca gtggcgataa ctttatgccc ttgttgaata aaacgacgag
     5101 taatgcattc accaaaacct gccgttgctc cagttactaa aacgatcatc tcactgttcc
     5161 tcaacgcttt tgtgtgtcaa taacatagca cgtgatgctt aagagcgggt aatactggtt
     5221 tgcaagattg cactccgtta tcagtaagcc tacgctatgg aacacctcgc gttcaggaga
     5281 agaaaatgac aacaatgaat cctttccttg tgcaaagcac actgccgtat ctggctcccc
     5341 attttgatca aattgccaac catcactatc gcccggcatt cgatgaggga atacagcaaa
     5401 agcgggcaga aatagctgcc atcgcgctta acccgcaaac acctgatttc aacaatacta
     5461 ttctggcact ggaacaaagc ggagaattac ttacccgcgt taccagcgtc ttttttgcga
     5521 tgactgcggc gcataccaat gatgaattac agcgtcttga tgaacagttt tccgctgaac
     5581 tggcggaact tgcgaatgat atctatctga acggggaatt attcgcgcgg gtagatgctg
     5641 tctggcaacg ccgtgaatcc ctggggcttg atagtgaatc catccgcctg gtggaggtga
     5701 ttcatcaacg ttttgtcctt gccggagcca aacttgagca agctgataaa gcaaaattaa
     5761 aagtactgaa tacagaagct gcgaccctga ccagccagtt taaccagcgg ttactggcag
     5821 caaataaatc cggcggtctg gttgtgaacg atatcgcgca gctggcagga atgagtgaac
     5881 aagagattgc gctggcggca gaggcggctc gcgagaaagg tctggataac aactggctga
     5941 ttccgctgct gaataccacc cagcaaccag cgcttgctga actgcgcgat cgcgcgacgc
     6001 gtgaaaaact gtttactgcg ggctggacgc gagcggaaaa aaatgatgcc aatgataccc
     6061 gcactatcat tcaacgtctg gtggagatcc gcgcgcagca ggcgaaactg cttggttttc
     6121 ctcattatgc cgcatggaaa atcgccgatc agatggcaaa aacgccagaa gcagcgctca
     6181 actttatgcg ggaaattgtt ccagcggcgc gtcaacgtgc gagcgatgaa ttagcctcga
     6241 tacaggcgat tatcgataag cagcagggcg ggtttagcgc gcagccgtgg gactgggcat
     6301 tttatgccga gcaggtaaga cgtgagaaat tcgatctcga tgaagcgcag ctcaagcctt
     6361 atttcgaatt aaacacggtg ttgaatgaag gcgtattctg gactgcgaat cagctcttcg
     6421 gtattaagtt tgtcgaacgt tttgatattc ctgtctacca tcctgacgtt cgtgtgtggg
     6481 aaatttttga tcataatggc gtggggctgg cgttatttta cggtgatttc ttcgcccgtg
     6541 attcaaaaag cggcggcgca tggatgggca attttgttga gcaatcaacg ctgaatgaaa
     6601 cgcacccggt aatttataac gtctgcaatt atcagaaacc cgctgccggt gagcctgcgt
     6661 tgttactctg ggatgatgta ataaccttat tccatgaatt tggtcatacg ctgcacggcc
     6721 tttttgcccg ccagcgttat gccacgcttt ccggcaccaa cacgccgcgt gattttgtcg
     6781 aatttccgtc gcaaatcaac gaacactggg caacacatcc gcaggtattc gctcgctacg
     6841 cccggcatta tcagagcaga gccgcaatgc ctgacgaact tcaacagaaa atgcgtaatg
     6901 ccagcctgtt caataaaggg tatgagatga gcgaactgct tagcgccgca cttctcgata
     6961 tgcgctggca ttgtctggaa gaaaacgaag caatgcagga cgtcgatgat ttcgaattgc
     7021 gggcgctggt ggcggaaaat atggatcttc ctgctatacc gccacgctat cgcagcagtt
     7081 atttcgccca tatttttggt ggcggatatg ccgcaggtta ttacgcttat ctgtggacgc
     7141 aaatgttggc cgatgatggt taccagtggt ttgttgagca gggcggatta acgcgtgaaa
     7201 atgggcggcg ttttcgcgag gcgattcttt ccagaggtaa cagcgaagat ctggaacgcc
     7261 tgtatcgaca atggcgcggt aaggcacctc agattatgcc gatgctgcaa catcgtggct
     7321 tgaacgtata aacacaacca atggctggcc tggatttatc gccagaccag cctcgttata
     7381 gcagtaattg cagcagctgg gcgagggcaa acttcaccgc taatgcgaat gccgtttcgc
     7441 agtcgccagc aaaattactt tttttgtata cccttccgtt ataataattt tcaaattcag
     7501 ccggaccaga tgagcaacat tagcggcagt ctggataaga aaagcgcgcc gaccgtcgtt
     7561 cgggtcactc atttgcagaa ataaaaataa attagcctga tggcctgaac tactgggcca
     7621 ttagggtatt gggttatgct atttgtcatc cttttaattg cg
//
LOCUS       DADSYM010000182         7635 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2415
            SAMN08039579-rid11723173.denovo.184, whole genome shotgun sequence.
ACCESSION   DADSYM010000182 DADSYM010000000
VERSION     DADSYM010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039579
            Sequence Read Archive: SRR6362533
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7635)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7635)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980162.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 03:39:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,484
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 4,347
            CDSs (with protein)               :: 4,347
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 43 of 85
            Pseudo Genes (incomplete)         :: 37 of 85
            Pseudo Genes (internal stop)      :: 19 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7635
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039579-rid11723173.denovo.184"
                     /strain="LGE2415"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8302 N 95.9628 W"
                     /collection_date="2015-08-24"
     gene            118..453
                     /gene="eutS"
                     /locus_tag="J1Y83_003920"
     CDS             118..453
                     /gene="eutS"
                     /locus_tag="J1Y83_003920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutS"
                     /protein_id="HBA7632023.1"
                     /translation="MDKERIIQEFVPGKQVTLAHLIAHPGEELAKKIGVPDAGAIGIM
                     TLTPGETAMIAGDLALKAADVHIGFLDRFSGALVIYGSVGAVEEALSQTVSGLGRLLN
                     YTLCEMTKS"
     gene            466..945
                     /gene="eutP"
                     /locus_tag="J1Y83_003921"
     CDS             466..945
                     /gene="eutP"
                     /locus_tag="J1Y83_003921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization acetate kinase EutP"
                     /protein_id="HBA7632024.1"
                     /translation="MKRIAFVGTVGAGKTTLFNALQGNYTLARKTQAVEFNDNGDIDT
                     PGEYFSHPRWYHALITTLQDVDMLIYVHGANDPESRLPAGLLDIGVSKRQIAVISKTD
                     MPDADVAATRKLLLETGFEEPIFELNSHDPQSVQQLVDYLASLTKQEEAGEKTHHSE"
     gene            920..1621
                     /gene="eutQ"
                     /locus_tag="J1Y83_003922"
     CDS             920..1621
                     /gene="eutQ"
                     /locus_tag="J1Y83_003922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization acetate kinase EutQ"
                     /protein_id="HBA7632025.1"
                     /translation="MKKLITANDIREAHARGEQAMSVVLRASIITPEAREVADLLGFT
                     ITECDESTAVTTSVPASVPADKTESQRIRETIIAQLPEGQFTESLVAQLMEKVMKEKQ
                     SLEQGALQPSFKSVTGKGGIKVIDGSSVKFGRFDGAQPHCVGLTDLVTGDDGSSMAAG
                     FMQWENAFFPWTLNYDEIDMVLEGELHVRHEGETMIAKAGDVMFIPKGSSIEFGTTSS
                     VKFLYVAWPANWQSL"
     gene            1618..2421
                     /gene="eutT"
                     /locus_tag="J1Y83_003923"
     CDS             1618..2421
                     /gene="eutT"
                     /locus_tag="J1Y83_003923"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization cob(I)yrinic acid
                     a,c-diamide adenosyltransferase EutT"
                     /protein_id="HBA7632026.1"
                     /translation="MKDFITEAWLRANHTLSEGAEIHLPADSRLTPSARELLESRHLR
                     IKFIDEQGRLFVDDEQQQPQPVHGLTSSDEHPQACCELCRQPVAKKPDTLTHLSAEKM
                     VAKSDPRLGFRAVLDSTIALAVWLQIELAEPWQPWLADIRSRLGNIMRADALGEPLGC
                     QAIVGLSDEDLHRLSHQPLRYLDHDHLVPEASHGRDAALLNLLRTKVRETETVAAQVF
                     ITRSFEVLRPDILQALNRLSSTVYVMMILSVTKQPLTVKQIQQRLGETQ"
     gene            2418..3434
                     /gene="pta"
                     /locus_tag="J1Y83_003924"
     CDS             2418..3434
                     /gene="pta"
                     /locus_tag="J1Y83_003924"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HBA7632027.1"
                     /translation="MIIKRCRELALRAPARVVFPDALDQRVLKAAQYLHQQGLATPIL
                     VANPFELRQFALSHGVAMDGLQVIDPHGNLAMREEFAHRWLARAGEKTPPDALEKLTD
                     PLMFAAAMVSAGKADVCIAGNLSSTANVLRAGLRIIGLQPGCKTLSSIFLMLPQYSGP
                     ALGFADCSVVPQPTAAQLADIALASAETWRAITGEEPRVAMLSFSSNGSARHPCVANV
                     QQATEIVRERAPKLVVDGELQFDAAFVPEVAAQKAPASPLQGKANVMVFPSLEAGNIG
                     YKIAQRLGGYRAVGPLIQGLAAPMHDLSRGCSVQEIIELALVAAVPRQTEVNRESSLQ
                     TLVE"
     gene            3473..3766
                     /gene="eutM"
                     /locus_tag="J1Y83_003925"
     CDS             3473..3766
                     /gene="eutM"
                     /locus_tag="J1Y83_003925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutM"
                     /protein_id="HBA7632028.1"
                     /translation="MEALGMIETRGLVALIEASDAMVKAARVKLVGVKQIGGGLCTAM
                     VRGDVAACKAATDAGAAAAQRIGELVSVHVIPRPHGDLEEVFPIGLKGDSSNL"
     gene            3873..4160
                     /gene="eutN"
                     /locus_tag="J1Y83_003926"
     CDS             3873..4160
                     /gene="eutN"
                     /locus_tag="J1Y83_003926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutN"
                     /protein_id="HBA7632029.1"
                     /translation="MKLAVVTGQIVCTVRHHGLAHDKLLMVEMIDPQGNPDGQCAVAI
                     DNIGAGTGEWVLLVSGSSARQAHKSETSPVDLCVIGIVDEVVSGGQVIFHK"
     gene            4172..5575
                     /locus_tag="J1Y83_003927"
     CDS             4172..5575
                     /locus_tag="J1Y83_003927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase EutE"
                     /protein_id="HBA7632030.1"
                     /translation="MNQQDIEQVVKAVLLKMQSSDTPPAAVHEMGVFASLDDAVAAAK
                     VAQQGLKSVAMRQLAIAAIREAGEKHARDLAELAVSETGMGRVEDKFAKNVAQARGTP
                     GVECLSPQVLTGDNGLTLIENAPWGVVASVTPSTNPAATVINNAISLIAAGNSVIFAP
                     HPAAKKVSQRAITLLNQAIVAAGGPENLLVTVANPDIETAQRLFKFPGIGLLVVTGGE
                     AVVEAARKHTNKRLIAAGAGNPPVVVDETADLARAAQSIVKGASFDNNIICADEKVLI
                     VVDSVADELMRLMEGQHAVKLTAEQAQQLQPVLLKNIDERGKGTVSRDWVGRDAAKIA
                     AAIGLKVPEQTRLLFVETTAEHPFAVTELMMPVLPVVRVANVADAIALAVKLEGGCHH
                     TAAMHSRNIENMNQMANAIDTSIFVKNGPCIAGLGLGGEGWTTMTITTPTGEGVTSAR
                     TFVRLRRCVLVDAFRIV"
     gene            5586..6422
                     /gene="eutJ"
                     /locus_tag="J1Y83_003928"
     CDS             5586..6422
                     /gene="eutJ"
                     /locus_tag="J1Y83_003928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization protein EutJ"
                     /protein_id="HBA7632031.1"
                     /translation="MAHDEQWLTPRLQTAATLCNQTPAATESPLWLGVDLGTCDVVSM
                     VVDRDGQPVAVCLDWADVVRDGIVWDFFGAVTIVRRHLDTLEQQFGRRFSHAATSFPP
                     GTDPRISINVLESAGLEVSHVLDEPTAVADLLQLDNAGVVDIGGGTTGIAIVKKGKVT
                     YSADEATGGHHISLTLAGNRRISLEEAEQYKRGHGDEIWPAVKPVYEKMADIVARHIE
                     GQGITDLWLAGGSCMQPGVAALFCKQFPALQVHLPQHSLFMTPLAIASSGREKAEGIY
                     AK"
     gene            6412..7599
                     /gene="eutG"
                     /locus_tag="J1Y83_003929"
     CDS             6412..7599
                     /gene="eutG"
                     /locus_tag="J1Y83_003929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization ethanol dehydrogenase
                     EutG"
                     /protein_id="HBA7632032.1"
                     /translation="MQSELQTALFQAFDTLNLQRVKTFSVPPVTLCGPGAVSSCGQQA
                     QTRGLKHLFVMADSFLHQAGMTAGLTRSLAVKGIAMTLWPCPVGEPCITDVCAAVAQL
                     RESGCDGVIAFGGGSVLDAAKAVALLVTNPDSTLAEMSETSVLQPRLPLIAIPTTAGT
                     GSETTNVTVIIDAVSGRKQVLAHASLMPDVAILDAALTEGVPSHVTAMTGIDALTHAI
                     EAYSALNATPFTDSLAIGAIAMIGKSLPKAVGYGHDLVARESMLLASCMAGMAFSSAG
                     LGLCHAMAHQPGAALHIPHGLANAMLLPTVMEFNRMVCRERFSQVGRALRTKKSDDRD
                     AINAVSELIAEVGIGKRMGDVGATSAHYGAWAQAALEDICLRSNPRTASLEQIVGLYA
                     AAQ"
BASE COUNT         1709 a         1997 c         2319 g         1610 t
ORIGIN      
        1 aatatctctc cattcttagt gatctacctc accttttaaa cgcgcttgcc gaattttgtt
       61 atttactctg acgaaaaatt gtcacgatac acgaaagttt ttcacaggcg gcgactcatg
      121 gataaagaac gcatcattca ggaatttgtg cccggtaaac aggtcacact ggcgcatctc
      181 atcgcgcatc ccggcgagga actggcgaag aaaatcggcg ttccggatgc gggcgcaatc
      241 ggcattatga cgctaactcc cggagaaacg gcgatgatcg ccggtgattt agcgttgaaa
      301 gccgccgatg tgcatatcgg ttttctcgac cgttttagcg gcgcgctggt gatttatggt
      361 tcggtgggcg cagtagaaga agcgttatca caaaccgtca gtggtttagg acgcttatta
      421 aattatacgc tttgtgaaat gaccaaaagt taatatgggg cattcatgaa acgaattgcg
      481 tttgtgggta ccgtcggggc aggaaaaaca acgttattta atgcgttgca ggggaattat
      541 accctcgcaa gaaaaacaca ggccgtggaa tttaatgata atggcgatat tgatacgccg
      601 ggtgaatatt ttagccatcc ccgctggtat cacgccttaa ttaccacgct acaggatgtg
      661 gacatgctga tctatgtcca cggcgcgaat gacccggaaa gtcggttgcc tgccgggttg
      721 ctggatattg gcgtcagcaa acggcaaatc gccgtcatta gtaaaacgga catgccagat
      781 gctgacgtcg ccgcaacgcg aaagttattg cttgagacgg gcttcgaaga gccgattttc
      841 gagctaaaca gccacgaccc gcaaagcgtg cagcaactgg tggattatct ggcatctctc
      901 accaaacagg aggaagcagg tgaaaaaact catcacagcg aatgatattc gtgaagcaca
      961 cgcgcgcggc gaacaggcaa tgtccgtggt tctgcgcgcc agcattatta ccccggaagc
     1021 ccgtgaagtg gcggatctgt tgggttttac catcaccgaa tgtgatgaat cgactgcggt
     1081 aacgacgtcc gttcccgcta gcgtgccagc cgataaaacc gaaagccagc gcatccgtga
     1141 aaccattatc gcccaactgc cggaaggtca gtttaccgaa agcctggtcg cgcagctgat
     1201 ggaaaaagtg atgaaggaaa aacagtcgct ggaacagggt gcactgcagc cgagctttaa
     1261 atcggtgacc ggcaaaggcg gcatcaaagt tattgatggc agcagcgtca agtttggtcg
     1321 tttcgacggc gcacaaccgc actgcgtagg cttgactgat ctcgtgaccg gagacgacgg
     1381 cagcagtatg gccgctggtt tcatgcagtg ggagaacgcc ttctttccgt ggaccctgaa
     1441 ctacgacgaa atcgacatgg tgctggaagg ggagctgcat gttcgccacg aaggcgaaac
     1501 tatgatcgcc aaagcgggcg atgtgatgtt tatcccgaaa ggctccagca tcgaatttgg
     1561 tacgacatcc agcgtgaaat tcctgtatgt cgcctggccg gctaactggc aatccctatg
     1621 aaagatttca tcaccgaagc atggctaaga gcgaaccata cgctcagcga aggcgcagag
     1681 atccatctcc ctgcggacag tcgcctgacg ccctctgccc gggagttact ggaaagccgc
     1741 catctgcgca tcaagtttat tgacgagcag ggccgcctgt ttgttgacga tgaacagcag
     1801 cagccgcagc ccgttcacgg gctgaccagt agcgatgaac atccgcaggc gtgctgcgaa
     1861 ctgtgccgcc agccggtagc aaaaaagccg gatacgctga ctcacctgtc ggcggaaaaa
     1921 atggtcgcca aaagcgatcc gcgtctgggt tttcgcgctg ttctcgacag caccattgcg
     1981 ctggcagtgt ggctgcaaat tgaactggcg gaaccgtggc agccgtggct ggcggatatc
     2041 cgttcgcgtc ttggcaacat tatgcgtgcc gatgcgctgg gggaaccgtt gggctgtcag
     2101 gcgattgtcg ggctttctga cgaagatctg catcggcttt ctcaccagcc gctgcgttat
     2161 ctcgaccacg atcatctggt gccggaagcc agccacgggc gcgatgcggc gttgctgaat
     2221 ctgttacgca ccaaagtgcg ggaaaccgaa accgtagcgg cgcaggtctt tatcacccgc
     2281 agctttgaag tattacgccc ggacattttg caggcgctga accgcctttc cagcacggtc
     2341 tacgtgatga tgatcctgag cgtaacgaag cagccgctaa cggtgaagca gatccaacaa
     2401 cgactggggg aaacgcaatg attattaaac gttgtcgtga actggcgttg cgagcgcccg
     2461 ccagagtagt ttttccggat gcgttagatc aacgtgtgct gaaagctgcg caatatttac
     2521 atcaacaagg tctggcaacg cccattctgg tcgccaatcc gtttgaactt cgtcagtttg
     2581 cgctcagtca cggcgtggcg atggacgggc tacaggtgat agatccgcat ggcaacctcg
     2641 caatgcggga agaatttgct catcgctggc tggcccgcgc gggcgaaaaa acgccgccgg
     2701 atgcgctgga aaaacttacc gatccgctga tgttcgccgc cgcaatggtc agcgccggta
     2761 aagcggatgt ctgtatcgcg ggcaacctct cttccacggc gaatgtgctg cgtgccggat
     2821 tacgcattat cggcttgcag ccaggctgta aaacgctctc atccattttc ctgatgctgc
     2881 cacagtacag cggtccggcg ttgggctttg ccgattgcag cgtggtgccg cagccgacgg
     2941 cggcacaact ggcggatatc gcgcttgcca gcgccgaaac ctggcgcgcc atcaccggag
     3001 aagaaccgcg cgtggcgatg ctgtcgtttt ccagtaacgg tagtgcccgt cacccctgcg
     3061 ttgccaacgt ccagcaggcg acagaaatcg tccgtgagcg cgcaccaaag ctggttgtcg
     3121 atggcgagtt gcagtttgac gccgccttcg tgccggaagt ggcggcgcaa aaagcgcctg
     3181 ccagcccgtt acagggcaaa gctaatgtga tggtttttcc gtcgctggag gcaggaaata
     3241 ttggctacaa aatcgcacaa cgacttggcg gatatcgtgc cgtcgggcca ttgatacaag
     3301 gacttgccgc gccgatgcac gatctctctc gtggttgtag cgtgcaggaa attatcgaac
     3361 tggcgctggt ggcagctgtg ccgcgtcaga cagaagtgaa ccgcgaaagc agtttacaaa
     3421 cactggttga atgaacggtc ccgttctgga ccctgataaa gaggaaagca caatggaagc
     3481 attaggaatg atcgaaaccc ggggcctggt tgcactgatt gaggcttctg acgcgatggt
     3541 taaagctgcg cgcgtcaagc tggttggtgt taagcagatt ggcggcggcc tgtgtactgc
     3601 catggttcgt ggtgatgttg cagcctgtaa agccgcgacc gatgccggtg ccgcagcggc
     3661 acagcgtatc ggcgagctgg tatctgtaca cgttatcccg cgtccgcacg gcgacctgga
     3721 agaagtgttc ccgatcggcc tgaaaggcga tagcagcaac ctgtaatcac cctgtgtgac
     3781 gtcgattcgg gcgtcacgct ctctcctttt tcattttacc ttctgcggag ggtaggggga
     3841 gcttttacct gaaatacgcc acggaggcgg gtatgaaact ggcagtcgtc actggacaaa
     3901 ttgtttgtac cgtacgccat cacggactag cgcatgacaa attgctgatg gtggaaatga
     3961 ttgatccaca aggtaatcct gacgggcaat gcgccgtcgc catcgacaat attggcgcgg
     4021 gaaccgggga gtgggtgtta ctggtgagtg gcagttccgc ccgccaggcg cataaaagcg
     4081 aaacgtcacc ggtcgatctg tgcgtgattg gcattgtcga tgaggtggtg tctggcggtc
     4141 aggtcatttt ccacaaataa ggcagaacat catgaatcaa caggatattg aacaggtggt
     4201 gaaagcggta ctgctgaaaa tgcaaagcag tgacacgccg cccgccgccg ttcatgagat
     4261 gggcgttttc gcgtccctgg atgacgccgt tgcggcagcc aaagtcgccc agcaagggtt
     4321 aaaaagcgtg gcaatgcgcc agttagccat tgctgccatt cgtgaagcag gcgaaaaaca
     4381 cgccagagat ttagcggaac ttgccgtcag tgaaaccggc atggggcgcg ttgaagataa
     4441 atttgccaaa aacgtcgctc aggcgcgcgg cacaccaggc gttgagtgcc tctctccgca
     4501 agtgctgact ggcgacaacg gcctgaccct aattgaaaac gcaccctggg gcgtggtggc
     4561 ttcggtgacg ccttccacta acccggcggc aaccgtaatt aacaacgcca tcagcctgat
     4621 tgccgcgggc aacagcgtca tttttgcccc gcatccggcg gcgaaaaaag tctcccagcg
     4681 ggcgatcacg ctgctcaacc aggcgattgt tgccgcaggc gggccggaaa acttactggt
     4741 taccgtagca aatccggata tcgaaaccgc gcaacgcttg ttcaagtttc cgggtatcgg
     4801 cctgctggtg gtgaccggcg gcgaagcggt agtggaagcg gcgcgcaaac acaccaataa
     4861 acgcctgatt gcggcaggtg ccggtaaccc gccggtagtg gtggatgaaa ccgccgacct
     4921 cgcccgtgct gctcagtcca tcgtcaaagg cgcttctttc gataacaaca tcatttgtgc
     4981 cgacgaaaag gtactgattg ttgtggatag cgtagccgat gaactgatgc gtctgatgga
     5041 aggccagcat gcggtgaaac tgaccgcaga gcaggcgcag caactgcaac cggtgttgct
     5101 gaaaaatatc gacgagcgcg gaaaaggcac cgtcagccgt gactgggttg gtcgcgatgc
     5161 ggcgaaaatc gcggcggcaa tcggccttaa agttccggaa caaacgcgac tgctgtttgt
     5221 agaaaccacc gcagaacatc cgtttgccgt gaccgaactg atgatgccgg tgctgcccgt
     5281 cgtgcgcgtc gccaacgtgg cggatgccat tgcgctggcg gtgaaactgg aaggcggttg
     5341 ccaccacacg gcggcaatgc actcgcgcaa tatcgaaaac atgaaccaga tggcgaacgc
     5401 cattgatacc agcattttcg ttaagaacgg accgtgtatt gccgggctgg ggctgggcgg
     5461 ggaaggctgg accacgatga ccatcaccac gccaaccggt gaaggggtaa ccagcgcgcg
     5521 gacgtttgtc cgtctgcgtc gctgtgtatt agtcgatgcg tttcgcattg tttaaggagt
     5581 aaccgatggc gcacgatgaa caatggctca ccccacgtct gcaaacggcg gcaacgctgt
     5641 gtaaccagac gcctgccgcg acggaatctc cgctgtggct gggggtggat ctgggcactt
     5701 gcgatgtggt gtcgatggtt gtcgaccgcg acggtcagcc ggtggcggta tgcctcgact
     5761 gggccgatgt ggtccgcgac ggcatcgtct gggatttctt cggtgcggtc accattgttc
     5821 gtcgccatct cgacaccctt gagcagcaat tcggtcgccg ttttagccat gcggcgacct
     5881 catttcctcc tggtacggac ccgcgtattt ccatcaacgt gctggagtct gccggactgg
     5941 aagtgagcca tgtgctggat gagccaaccg cagttgcgga tctgctgcaa ctggacaacg
     6001 ccggtgtagt ggatatcggc ggtggcacta ctggtattgc catcgtgaaa aagggcaagg
     6061 tgacgtactc ggcggatgaa gcgaccggcg gacatcacat ctctctgact ctcgccggaa
     6121 atcgtcgtat ttcgctggaa gaggcagagc agtacaagcg cggtcacggt gacgagattt
     6181 ggcccgcggt gaaaccggtg tacgaaaaaa tggcggacat tgtcgcccgg catattgaag
     6241 ggcagggcat tactgattta tggctggcgg gcggttcctg tatgcaaccg ggcgtagcgg
     6301 cgctgttttg caaacaattt ccggcgttac aggtgcattt accgcagcac agtttgttta
     6361 tgaccccgct ggcaatcgcc agtagtgggc gagagaaagc ggaggggatc tatgcaaagt
     6421 gaattgcaaa cggcgctttt tcaggcgttc gataccctga atctgcaacg ggtaaaaaca
     6481 tttagcgttc caccggtgac gctttgcggt ccgggcgcgg tgagcagttg cgggcagcaa
     6541 gcgcaaacgc gtgggttgaa acatctgttc gtgatggcag acagcttttt gcatcaggca
     6601 gggatgaccg ccgggctgac acgtagcctg gccgttaaag gtatcgccat gacgctctgg
     6661 ccatgtccgg tgggcgaacc gtgcatcacc gacgtgtgtg cagctgtggc gcagttgcgt
     6721 gaatcaggtt gtgacggggt gatcgcgttt ggcggcggct cggtgctgga tgcggcgaaa
     6781 gccgtggcgt tgctggtgac gaacccggat agcacgctgg cagaaatgtc agaaaccagc
     6841 gttctgcaac cgcgcctgcc gttgattgcc attccaacga ccgccggaac cggctctgaa
     6901 acgactaacg tgacggtgat tatcgatgcg gtaagcgggc gcaaacaggt gttagcccat
     6961 gcctcgctga tgccggatgt ggcgattctc gacgccgcgt tgaccgaagg cgtgccgtcg
     7021 catgtcacgg cgatgactgg cattgatgcg ttaacccatg ccattgaagc gtacagcgcg
     7081 ctgaacgcta caccgtttac cgacagcctg gcgattggtg ccattgcgat gattggcaaa
     7141 tcgctgccga aagcggtggg ctacggtcac gaccttgtcg cccgcgagag catgttgctg
     7201 gcttcatgta tggcgggaat ggcgttttcc agcgccgggc ttgggttatg ccacgcgatg
     7261 gcgcatcagc caggggcggc gctgcatatt ccgcacggtc tggcgaacgc catgttgctg
     7321 ccaacggtga tggagttcaa ccggatggtc tgtcgtgaac gctttagtca ggttggtcgg
     7381 gcgctgcgaa ctaaaaaatc cgacgatcgt gacgctatta atgcggtaag tgagctgata
     7441 gcggaagttg ggattggtaa acgaatgggc gatgttggtg cgacatctgc gcattacggc
     7501 gcatgggcac aggccgcgct ggaagatatt tgtctgcgca gtaacccgcg taccgccagc
     7561 ctcgagcaga ttgtcggcct gtacgcagcg gcgcaataaa tgccggatgc gacgcttgcc
     7621 gcgtcttatc cggcc
//
LOCUS       DADSYM010000183         7543 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2415
            SAMN08039579-rid11723173.denovo.185, whole genome shotgun sequence.
ACCESSION   DADSYM010000183 DADSYM010000000
VERSION     DADSYM010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039579
            Sequence Read Archive: SRR6362533
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7543)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7543)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980162.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 03:39:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,484
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 4,347
            CDSs (with protein)               :: 4,347
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 43 of 85
            Pseudo Genes (incomplete)         :: 37 of 85
            Pseudo Genes (internal stop)      :: 19 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7543
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039579-rid11723173.denovo.185"
                     /strain="LGE2415"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8302 N 95.9628 W"
                     /collection_date="2015-08-24"
     gene            complement(546..1298)
                     /locus_tag="J1Y83_003930"
     CDS             complement(546..1298)
                     /locus_tag="J1Y83_003930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HBA7632033.1"
                     /translation="MNLEALPKYYSPKSPKLSDDAPATGSGGLTITDVMAAQGMVQSK
                     APLGFALFLAKVGVQDPQFAIEGLLNYAMAMDNPTLNKLSEEIRLQIIPYLVNFAFAD
                     YSRSAASKARCEYCAGTGFHNVLREVVKHSRSGESVIKEEWVKELCQHCHGKGEVSTA
                     CRGCKGKGIVLDEKRTRLHSTPVYKVCGRCNGNRFSRLPTTLARCHVQKLVPDLTDYQ
                     WYKGYADVIDKLVTKCWQEEAYAEVQLRKVTR"
     gene            complement(1312..2361)
                     /locus_tag="J1Y83_003931"
     CDS             complement(1312..2361)
                     /locus_tag="J1Y83_003931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416078.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HBA7632034.1"
                     /translation="MRVLLRPVLVPELGLVVVKPGRESMPVFHNTRVLVEPEPKSMRN
                     LPSGVVPAVRQPLVEDKTLLPFFSNARVIRAAGGAGALSDWLLRHIKSCQWPHGDYHH
                     SETVIHRYGTGAMVLCWHCDNQLRDQTSESLEQLAHQNLSAWMIDVIGHAISGTQECE
                     LSLAELSWWAVRNQVADALPEAVLRRSLGLPAEKNCSVYRESDIVPGEQTATSILKQR
                     TKNLAPLPHAHQQQNPSQEKTVVSITVDPESPESFMKLPKRHRWVNEKYTRWVKTQPC
                     ACCGMPADDPHHLIGHGQGGMGTKAHDLFVLPLCRKHHDELHANTVAFEEKYGSQLEL
                     IFRFIDRALAIGVLA"
     gene            complement(2363..2641)
                     /locus_tag="J1Y83_003932"
     CDS             complement(2363..2641)
                     /locus_tag="J1Y83_003932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA7632035.1"
                     /translation="MARNVKYYNSDNSPVLACTHERYSHAFKSEWFQHPPCTEEQAEW
                     IIQCYRRRGYEVKKALSLDYRHWIISVRLPYSERPPRPSRTFQQRIWR"
     gene            complement(2708..2959)
                     /locus_tag="J1Y83_003933"
     CDS             complement(2708..2959)
                     /locus_tag="J1Y83_003933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA7632036.1"
                     /translation="MMNIEELRKIFCEDGLYAVCVENGNLVSHYRIMCLRKNGAALIN
                     FVDGRVTDGFILREGEFVTSLQALKEIGIKAGFSAFAEE"
     gene            complement(3176..3388)
                     /locus_tag="J1Y83_003934"
     CDS             complement(3176..3388)
                     /locus_tag="J1Y83_003934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Hok family toxin"
                     /protein_id="HBA7632037.1"
                     /translation="MLDTCRLASYVPKGKEKQAMKQQKAMLIALIVICLTVIVTALVT
                     RKDLCEVRIRTGQTEVAVFTAYEPEE"
     gene            3433..3554
                     /locus_tag="J1Y83_003935"
                     /pseudo
     CDS             3433..3554
                     /locus_tag="J1Y83_003935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_303812.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(3947..4612)
                     /locus_tag="J1Y83_003936"
     CDS             complement(3947..4612)
                     /locus_tag="J1Y83_003936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012421537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HBA7632038.1"
                     /translation="MTVADFKRPKLELPNGAKKLLLHSCCAPCSGEVMEALQASGIDY
                     TIFFYNPNIHPQKEYLIRKDENIRFAKQHGVPFIDADYDTDNWFERAKGMEWEPERGI
                     RCTMCFDMRFERTALYAAENGFSVISSSLGISRWKNMQQVNDCGRRAVAYYPGMVYWD
                     YNWRKQGGSSRMIEISKREQFYQQEYCGCVYSLRDTNLHRKSQGRPLIKIGQLHYGKE
                     GQE"
     gene            complement(4666..4899)
                     /locus_tag="J1Y83_003937"
     CDS             complement(4666..4899)
                     /locus_tag="J1Y83_003937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001366387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HBA7632039.1"
                     /translation="MSTSATILDMCCGSRMLWFDKNDEWSIFSDIRKEEHSLCDGRHP
                     IINPDIIADFRALPWLSALHQKQTLPDLDICFR"
     gene            complement(4896..5318)
                     /locus_tag="J1Y83_003938"
     CDS             complement(4896..5318)
                     /locus_tag="J1Y83_003938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF977 family protein"
                     /protein_id="HBA7632040.1"
                     /translation="MAKVFTQEEREKIKGQVVELVRQSGRETLRQLEAKTGATRYLMS
                     VLARELVASGDVYNSGYGLFPSEQARKDWQNARKKLSRAKVKKPAVVDPDLIWSLPDG
                     EIRRYDSRLNIICRECRKSEVMQRVLTFYQGNYREVML"
     gene            complement(5359..6324)
                     /locus_tag="J1Y83_003939"
     CDS             complement(5359..6324)
                     /locus_tag="J1Y83_003939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001373617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA7632041.1"
                     /translation="MSLLFAERPLVINTQLAMKIGLNEAIVLQQLHYWLRDTNSGMEC
                     DGVRWIYNTTEQWLAQFPFWSESTLKRAFASLKTLGLLRCEKLNKSKRDMTNFYTINY
                     GSDLLDGGKLSESISSKCAAPSGQNDTMEEVKMKRSIGSKRSNVIGSKWPDDLTENTT
                     EITTENKNTFRPEASQPDPQTTEQDFLTRNSDAVVFSAKKRQWGSREDLACAQWIWGR
                     IVGLYEQAASDDGEIMRPKEPNWTAWANDVRTMRMLDGRSHRQICEMFGRVQRDPFWV
                     KNIMSPSKLREKWDELVIRLGRSPVQRCVNHISEPDTEIPPGFRG"
     gene            complement(6305..6826)
                     /locus_tag="J1Y83_003940"
     CDS             complement(6305..6826)
                     /locus_tag="J1Y83_003940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001578395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA7632042.1"
                     /translation="MKITPEQAREALDAWICRPGMTQEQATILITEAFWALKERPNID
                     VQRVTYEGGAVDQRALSVNRVKIFERWKAIDTRDKREKFTALVPAIMEAIRISDFRLY
                     REISDGKSITYMIAGLNKEYGDVVESGLLFADPAVVDRETDELIEKVIAFKLAYRQQY
                     QQKAGWNYEPSFC"
     gene            complement(6810..7037)
                     /locus_tag="J1Y83_003941"
     CDS             complement(6810..7037)
                     /locus_tag="J1Y83_003941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HBA7632043.1"
                     /translation="MLKVDAITFFGSKTKLANAAGVRLASVAAWGILVPEGRAMRLQE
                     ASGGELQYDPKVYDEYRKTTRAGRLNNENHS"
     gene            7118..7525
                     /locus_tag="J1Y83_003942"
     CDS             7118..7525
                     /locus_tag="J1Y83_003942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000381212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBA7632044.1"
                     /translation="MDTRTLGQRVLARRKELRLTQREAARLAGVAHVTISQWERDETQ
                     PVGKRLFALADALKCSPTWLMFGDEDKAPVPAQELHVETELTPNHKELIELFDALPSS
                     EQEALLSEMRARVENFNKLFEEMLKARKNKSIK"
BASE COUNT         1935 a         1980 c         1589 g         2039 t
ORIGIN      
        1 atttataatt ttcatcctac tgttttccct gcactgccaa cacaagtttc atcgcaacac
       61 tctgacaaga cacctataaa atcagtttcg ttatcacctt aattttcttc atgggtaaca
      121 aaccggtaat acatcgatag ccagatttcc ggcgtaagcc atttatttgc agcctaattg
      181 atgcgcgtta ctgttacctc ttccctgcat caggatggct tctccagatg atatggttaa
      241 tcacactgga acattcacgc ataaccgttt ccttcatatc ctgccgtttt agcatgtctt
      301 ttccccttct tgttaatatc aataactaac acaaatctaa aaaagccagc aatgtaaaaa
      361 aactgcattc acggcaacca taatggttac cgttctgcac aaagtttatg tccacaaaaa
      421 aaccactctg cgcggcttat tgatattttc cacaggtatg catataaggc tcatcattgg
      481 aaaactcttt gcacgtttcc ttagaaaata caatcgttat gtcgccatcg ttcggtgaaa
      541 atcatttatc tcgtcacttt tcttaattgc acctcagcat atgcttcttc ctgccagcac
      601 tttgtaacca gtttatcaat gacatctgca tatcctttgt accactgata atccgtcagg
      661 tctggtacca gcttctggac atgacaccgc gccagtgtcg ttggtaaacg actaaaccgg
      721 tttccattgc aacgcccaca aaccttataa acaggcgtgc tatgaagccg ggttcttttt
      781 tcatccagga caataccttt ccccttacac cctctgcacg ctgtgctgac ttctccctta
      841 ccatggcaat gctgacatag ttccttcacc cactcttcct tgataacaga ttccccactt
      901 ctggagtgtt tcaccacttc gcgcaataca ttatgaaatc cagtaccagc acaatactca
      961 cagcgagcct tacttgccgc agacctggaa taatcagcaa aggcaaaatt cacaaggtaa
     1021 gggatgatct gtaaccggat ttcttcactc aatttgttca atgtcggatt atccattgcc
     1081 atcgcgtaat tgagcagacc ttcaatcgca aactgaggat cctgaacacc aacttttgcc
     1141 aggaataagg caaaaccaag cggagctttc gactgcacca tcccctgcgc agccatcaca
     1201 tctgttattg ttaaaccacc cgagcctgtc gccggtgcgt catcactcaa ttttggagat
     1261 tttggggagt aatattttgg taaagcttca aggttcatgc tcgttctcca cttacgccag
     1321 tacgcctatt gccagcgcac gatcgataaa acgaaatatc agctccagct gggagccata
     1381 cttctcttca aatgccacgg tattcgcatg cagctcgtcg tgatgctttc tgcacaaagg
     1441 caacacaaag agatcatgtg ctttggttcc cattccgccc tgcccgtgac caatcagatg
     1501 atgcggatca tcggctggca taccgcagca agcacacggc tgtgtcttaa cccagcgtgt
     1561 gtacttttca ttaacccaac ggtgacgttt aggcagcttc atgaaagatt ccggagactc
     1621 tggatcaacg gtgatgctta ccaccgtctt ttcctgtgat gggttttgtt gctggtgggc
     1681 gtgaggcaac ggtgcaagat tttttgtgcg ctgtttcaat atgctggtgg cggtctgctc
     1741 tcccggtacg atgtcgcttt cgcggtacac cgagcagttt ttttccgctg gtaatcccag
     1801 cgagcgacgt aataccgctt ccggtagcgc gtccgccacc tgattgcgga ccgcccacca
     1861 ggataattca gccagagata attcacactc ctgcgtaccg cttattgcgt gaccgatgac
     1921 gtcaatcatc catgctgaca ggttttgatg agcaagttgc tcgagtgatt cggatgtctg
     1981 gtcacgcagc tggttgtcgc agtgccagca caacaccatt gcgccggtac cataacggtg
     2041 aataacggtt tcgctgtgat gataatcgcc gtgtggccac tggcaggatt taatatggcg
     2101 caacagccag tcagacaatg caccagcacc accagcagca cgaattaccc gtgcgttact
     2161 gaaaaacggc agcaatgttt tgtcttccac cagcggctgg cgaacggcag gaacgacccc
     2221 ggacggcaga ttacgcatgc ttttcggttc cggctccacc agtacccggg tattgtggaa
     2281 taccggcatg gattcacggc ccggcttaac gaccaccagc ccgagttccg gtaccagaac
     2341 aggtcgaagt aatacccgca cgttacctcc agatgcgttg ctggaatgta cgggaaggac
     2401 gcggtgggcg ttcggagtaa ggcaatctga ctgagattat ccagtgacgg tagtcgagac
     2461 taagggcttt cttaacctcg tatccgcgcc tgcggtaaca ctgaattatc cattcagcct
     2521 gctcttcagt gcatggaggg tgctggaacc attcagactt gaatgcgtga gaataccgct
     2581 cgtgcgtgca ggcaagaacg ggcgaattat cagaattgta atattttacg ttgcgtgcca
     2641 tcggttttct ccggtggcac ggtgttactc agcgggagtt cagccccgcg caagattgta
     2701 gatgagttta ttcttctgca aaagctgaaa agcctgcttt tattccgatc tctttcagtg
     2761 cctgtaatga agtgacaaac tcaccttcgc gcaagataaa tccgtctgtc actcgaccat
     2821 ccacaaaatt aattaacgca gccccattct ttcgcaaaca cataatgcgg taatgactaa
     2881 caagatttcc attttcaacg cacacagcat agaggccatc ttcacaaaaa attttacgca
     2941 gttcttcgat gttcatcatc agaatccttc cggataatta gctctcccct ttaagggacc
     3001 atccctctta tccctgcgcg ctacttaagt atttttgatt ctattccggc accgtccaga
     3061 acttcaaacg cgttgaaaat aaaaacaaaa acccgccgaa gcgggttaag tgcgggtgcg
     3121 ttgaggatgc ctgccacatc agaggtggcg agggatttct ccctcgccgg gtctcttact
     3181 cctcaggttc gtaagctgtg aagacagcga cctccgtctg gccggttcgg attcgtacct
     3241 cgcagaggtc tttcctcgtt accagtgccg tcactatgac ggttaaacaa atgacgatca
     3301 gggcgattaa catcgccttt tgctgcttca tagcctgctt ctccttgcct ttcggcacgt
     3361 aagaggctaa cctacatgtg tctagcatga aattggcctc agattaatgt taagcgtctt
     3421 gcaggacgcg taatgttaac tggggctttt ctctatctgc cgttggtgtt catgcccgag
     3481 gcagatagcc tcaagcaccc gcagccattc tacttaacta ccgttacctc gccaatatga
     3541 aatcaatcag taaggtgatc cataagaaca ataacaagac aataaattgc cattacagcc
     3601 acaatagcca gcgtgcattt gagaaccagc acataaatct cctgctcttg acgtatacag
     3661 gcactgataa atatgtgtct tcgtgctcgt tacttcaata cgtaagcgtc agttgcgcgt
     3721 aatcaaatca gacaaccgac tttaaaccag ggacaaacag caggtaaaat aatcaaacgt
     3781 tattttaagc catatcatgg cttcaagatt tcctctaatt tcaacaagat gagcaggtca
     3841 ttgcttgcac tgatgacctg ctcattaatc atgattattt gaaaattaag gtaggcgtac
     3901 tatatataga aacactgcta cgctccgttg ttggcataat ataaaatcat tcctgccctt
     3961 ctttaccgta gtggagttga ccaattttga ttagagggcg tccctgagat ttgcggtgta
     4021 gattggtatc gcgcagtgaa tacacgcagc cacaatattc ctgctgatag aattgttcgc
     4081 gcttgctgat ttcaatcatg cgggaagagc cgccctgctt tcgccagtta taatcccagt
     4141 acaccatgcc cggataatac gcgacggctc gccgtccaca gtcgttaacc tgctgcatat
     4201 ttttccagcg tgaaatgccc agtgagctac tgatcacact gaaaccattt tcagcggcat
     4261 acagcgcagt ccgctcaaaa cgcatgtcaa aacacatggt acaacggatc cctcgttcag
     4321 gctcccattc cattcctttg gctcgctcaa accagttgtc tgtgtcgtaa tcagcatcaa
     4381 taaatggcac gccgtgttgt ttagcaaagc gaatattctc atccttacga attaaatact
     4441 ctttctgagg atgaatgttc gggttgtaga aaaagatggt gtagtcgatt cccgaggcct
     4501 gaagcgcctc catcacttca ccggaacatg gagcacagca agagtgcagt aatagttttt
     4561 ttgccccgtt tgggagctcc aatttggggc gtttgaaatc agcaacagtc ataaatgtgt
     4621 ttatgaggtt catgaaaata gcaaaaagtg tagcatcaga acgggctatc ggaaacagat
     4681 gtctaaatct ggtaatgtct gcttttgatg caaagcagac aaccacggca gcgcacgaaa
     4741 atcagcgata atgtcgggat taataatcgg gtgacgtcca tcacatagcg aatgttcctc
     4801 tttcctgata tcgctaaata tcgaccattc gtcattctta tcgaaccaga gcatgcgact
     4861 gccacagcac atgtcgagaa tggtagccga tgtactcaca gcatcacctc ccgataattc
     4921 ccctgataaa acgtcagaac acgctgcata acttcgctct tccggcactc gcgacagatt
     4981 atgtttagac gactgtcgta gcggcgtatt tctccatcag gtaatgacca gataaggtcc
     5041 ggatcaacca cagcaggttt cttcaccttt gccctcgaga gttttttgcg ggcgttttgc
     5101 cagtccttac gcgcctgttc agacgggaat aacccgtagc cggagttgta tacatcgcca
     5161 ctggctacca gctctctggc aagaacgctc atcagatatc tagtcgcacc tgttttagct
     5221 tccagttgcc gtaacgtctc acgaccgctc tggcgcacga gttccaccac ctgcccttta
     5281 attttttccc gctcttcttg tgtaaaaact tttgccacaa gtcctcctta aaattacctc
     5341 atgacctgaa atcaacattt atcctctgaa accaggtgga atttctgtat ccggttcaga
     5401 aatatgatta acacaacgct gtacaggtga acgtcccagg cggatgacca gttcgtccca
     5461 tttttcgcgg agctttgacg ggctcatgat gttttttacc cagaatggat ctcgctgaac
     5521 ccgaccaaac atttcgcaaa tttgtctgtg gcttctgcca tccagcatcc gcattgtgcg
     5581 cacgtcattg gcccaggcag tccagttagg ctcttttggt cgcatgatct cgccatcatc
     5641 actggctgcc tgttcgtaga gacccacgat ccgcccccag atccactgcg cacacgccaa
     5701 atcctccctg ctaccccact ggcgtttttt cgcactaaac acaaccgcgt cggagttccg
     5761 ggttaaaaaa tcctgttcag tcgtctgcgg gtccggttgc gaagcttccg gacgaaaagt
     5821 gtttttattc tctgtagtaa tctctgttgt attctctgta agatcatcag gccattttga
     5881 cccgatgaca ttggatcgtt ttgaaccaat ggagcgtttc attttgacct cttccatcgt
     5941 gtcattttga cctgatggag cggcgcattt tgaactgatg gattcgctca atttgccacc
     6001 atctaaaaga tcgctcccgt agttgatcgt gtagaaattg gtcatgtcgc gctttgattt
     6061 attgagcttt tcgcaacgca aaagccccag cgttttcaga cttgcaaacg cgcgctttaa
     6121 cgttgactct gaccagaacg ggaactgcgc cagccattgt tccgttgtgt tgtaaatcca
     6181 gcgaacacca tcacattcca tgccggaatt ggtatctctc aaccagtaat gcagttgctg
     6241 caacacaatg gcttcgttta agccaatctt catcgcaagc tgtgtgttta taaccagtgg
     6301 gcgttcagca aaaagaaggc tcataattcc atccagcttt ttgttggtat tgctgtcgat
     6361 acgcaagctt gaaagcaatt actttttcta taagttcgtc agtttcacga tctacaacgg
     6421 caggatctgc aaaaagcagt ccggactcca ccacatcgcc atattcttta tttaacccag
     6481 cgatcatgta cgtaatgctc tttccgtcac taatttcacg atacaacctg aaatcactaa
     6541 tccggatagc ctccataatt gcaggcacta gcgctgtgaa cttttcacgc ttatccctgg
     6601 tgtcgatagc cttccagcgt tcaaatatct tcactcgatt aacgctaagc gctcgctgat
     6661 caaccgcgcc accttcatat gtgacacgct gaacatcgat gttcgggcgc tctttcaaag
     6721 cccagaatgc ttcagtgatt aatatcgtcg cctgctcctg tgtcattcct ggtcgacata
     6781 tccaggcatc cagagcctca cgagcctgtt caggagtgat tttcattgtt caaccgcccc
     6841 gcccgcgtcg tcttacgata ttcgtcataa actttgggat catactgaag ctccccgcca
     6901 gatgcctcct gtagacgcat cgcgcgacct tcaggaacca gtatccccca agcagcaaca
     6961 cttgccagtc tcactcctgc ggcattggca agctttgttt tgctgccaaa aaaagtaatt
     7021 gcgtcaactt taagcatcaa agccccctct tgttagacat ttctaacatt attgtgcgcg
     7081 ggatacctaa gtcaagaaaa attagaatta cctaactatg gatacaagaa ccctaggcca
     7141 gcgagttctg gcgcgacgaa aagaattacg cttaacacaa cgagaagctg cgcgcctcgc
     7201 tggagttgct cacgtcacaa tttcacaatg ggaaagagac gaaacccagc cagtcggaaa
     7261 acgattgttt gctttagcgg atgctctgaa gtgctcacct acatggctaa tgtttggtga
     7321 cgaagacaag gcaccagtgc ctgcacaaga acttcatgtg gaaacagagc taactcccaa
     7381 ccacaaagaa ttgatcgaat tattcgatgc tcttccatct tccgagcagg aagccttgct
     7441 gtctgaaatg cgcgcaagag tagaaaactt caacaaactc ttcgaagaaa tgcttaaagc
     7501 gcgtaaaaat aaatcaataa aataacattt ttttcaagtg att
//
LOCUS       DADSYM010000184         7532 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2415
            SAMN08039579-rid11723173.denovo.186, whole genome shotgun sequence.
ACCESSION   DADSYM010000184 DADSYM010000000
VERSION     DADSYM010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039579
            Sequence Read Archive: SRR6362533
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7532)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7532)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980162.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 03:39:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,484
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 4,347
            CDSs (with protein)               :: 4,347
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 43 of 85
            Pseudo Genes (incomplete)         :: 37 of 85
            Pseudo Genes (internal stop)      :: 19 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7532
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039579-rid11723173.denovo.186"
                     /strain="LGE2415"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8302 N 95.9628 W"
                     /collection_date="2015-08-24"
     gene            complement(3..1310)
                     /locus_tag="J1Y83_003943"
     CDS             complement(3..1310)
                     /locus_tag="J1Y83_003943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HBA7632045.1"
                     /translation="MTDPILASCILIAVFVVLLAMGSPIGICIVIASFSTMMLVLPFD
                     VAMFSTAQKMFSSLDSFALLAVPFFVLSGVIMNSGGIASRLVNFAKLFTGKLPGSLSY
                     TNIVGNMMFGAISGSAIAASTSIGGVMVPMSAREGYDRSFAAAVNIASAPTGMLIPPT
                     TAFILYALASGGTSIAALFAGGLVAGLLWGIGCMLVTLIVAKRHNYRVFFTVQKGMVL
                     KVSVEAIPSLMLIVIIVGGIVQGIFTAIEASAIAVVYTLLLTVVFYRTLKLKDLPSIL
                     LQTVVMTGVIMFLLATSSAMSFSMSITNIPTALSNMILGISANKLVILLVITVFLLII
                     GAFMDIGPAILIFTPILLPIMIKLGVDPIHFGIIMIYNLAIGTITPPVGSGLYVGASV
                     GKVKVEEVIKPLLPFYAVIIGVLLLITYIPGMTLFLPRLLGVM"
     gene            complement(1303..1818)
                     /locus_tag="J1Y83_003944"
     CDS             complement(1303..1818)
                     /locus_tag="J1Y83_003944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HBA7632046.1"
                     /translation="MNTFRKGLDLLLGSVCCLMLAIMVGIACWQVISRYILDVPSTLT
                     EEMLRFSLVWVSMLGMAFVVGKKQHISLTILLDKAPTTLRRWWEIILQIIFIAFSVWV
                     LIIGGLRISVVSALQVSPALGISMSKIYYALPVSGVLIIIYSLLNIIESLKDKSSTTD
                     ATLHSLEKSHD"
     gene            complement(1837..2820)
                     /locus_tag="J1Y83_003945"
     CDS             complement(1837..2820)
                     /locus_tag="J1Y83_003945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HBA7632047.1"
                     /translation="MERSLFIRTVMLTLLFTVSFFSRGETTLRLAYAENSQPVKDALH
                     FFGQQVEEKTGGEIKVAYFPDGQLGGERELVELIQANAVDITKVSSGLMESFSPLYGI
                     FSLPYLFSNADEYYKVMDNPKIMDPVYQSTAAQGFIGIGWYDSGARNFYMSKSPIKSI
                     EDLRGKKIRVMQSDTSIQTLKLLGASPIAMSQAEVYTSLQQGILDGAENNEFALTIAR
                     HGEVARYYTYDMHTRIPDVLLMSTATLAKLTPEQRTIVKDAIRASIEFEKAAWDKEIE
                     KTKQAAVKDFNVEFFEIDKKPFQDAVQPIYDNLKNKPQLYKLYQQIQSAKH"
     gene            3599..4738
                     /locus_tag="J1Y83_003946"
     CDS             3599..4738
                     /locus_tag="J1Y83_003946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 105 protein"
                     /protein_id="HBA7632048.1"
                     /translation="MKVWPVKHSPLLRQPERLITQNELQALIRKITNNLVNIKDEAGQ
                     FLLRLDDGRVIDTKGWDGWEWTHGVGLYGIYQYYQQTGDSVMCDIIENWFSDRFAEGA
                     TTKNVNTMAPFLTLAYRYEDTGRPEYLPWLDSWAEWAMHTMPRTNHNGMQHITLAEEN
                     DQQMWDDTLMMTVLPLAKIGKLLNRNEYVEEAVYQFLLHVQNLQDRTTGLWFHGWSYD
                     GCHNFANARWARGNSWLTIVIPDFLELLNLPENNSVRRYLVQVLHTQIEALAKCQDES
                     GLWHTLLDDPNSYLESSATAGFAYGILKAVRKHYVAPLYAEVAEKAIRGIVKNISPEG
                     ELLQTSFGTGMGANLDFYREIPLTSMPYGQAMAVLCLTEYLRKYF"
     gene            complement(4942..5844)
                     /locus_tag="J1Y83_003947"
     CDS             complement(4942..5844)
                     /locus_tag="J1Y83_003947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HBA7632049.1"
                     /translation="MGSTRKGMLNVLIAAVLWGSSGVCAQYIMEQSQMSSQFLTMTRL
                     IFAGLILLTLSFVHGDKIFSIINNHKDAISLLIFSVVGALTVQLTFLLTIEKSNAATA
                     TVLQFLSPTIIVAWFSLVRKSRPGILVFCAILTSLIGTFLLVTHGNPTSLSISPAALF
                     WGIASAFAAAFYTTYPSMLIARYGTLPVVGWSMLIGGLILLPFYAGQGTNFVVNGSLI
                     LAFFYLVVIGTSLTFSLYLKGAQLIGGPKASILSCAEPLSSALLSLLLLGITFTLPDW
                     LGTLLILSSVILISMDSRRRARAA"
     gene            5886..6092
                     /locus_tag="J1Y83_003948"
     CDS             5886..6092
                     /locus_tag="J1Y83_003948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312620.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA7632050.1"
                     /translation="MIAKMARMAQFAPRRFFLAQTGELNIFNKQQANHCNTQHPYRQP
                     EQRVVAPDFNHNFVNRAGDNLADA"
     gene            6284..6413
                     /locus_tag="J1Y83_003949"
                     /pseudo
     CDS             6284..6413
                     /locus_tag="J1Y83_003949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001198208.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system contractile sheath
                     small subunit"
     gene            complement(6863..>7532)
                     /locus_tag="J1Y83_003950"
     CDS             complement(6863..>7532)
                     /locus_tag="J1Y83_003950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YjiK family protein"
                     /protein_id="HBA7632051.1"
                     /translation="SSLTYNKEDDFLYATQNSPAQLLKITKSGDIMDRAPLPFISDAE
                     TIEHIQGNIFAAVDEKTSELFFFTVTKDMHISFRNKIQLEKFNKKNRGFEGLAWKADD
                     RMLFVAKERRPSKIFIYQLSPDLLSVKQATIPEALNDIRVNDISGLAFNNESLMILSD
                     ESRKLLKFNLTEMSFIEMLDLTKGNHSLTSDLPQPEGIVTLPDESIYVASEPDILAKF
                     VPNK"
BASE COUNT         2290 a         1521 c         1652 g         2069 t
ORIGIN      
        1 ctttacataa cgccgagaag gcgaggcagg aataacgtca ttcctggtat ataagttatc
       61 agtagtaata caccaataat aacggcataa aaggggagta acggcttgat tacttcttca
      121 acctttacct ttccgacgct ggcaccgacg tataaaccac tgcctactgg tggagtaatc
      181 gtaccgatag ccaggttata aatcatgata ataccaaaat gaatgggatc aacacctagt
      241 ttaatcataa ttggcaacag gattggcgta aatataagaa tggctgggcc aatatccata
      301 aatgcaccga taataagcaa aaagacggtg attaccagca ggataactaa tttattggca
      361 gaaattccta aaatcatgtt gcttaacgct gtcggaatat ttgtaatcga catagagaaa
      421 gacatcgctg aagacgttgc cagaaggaac ataatcactc cagtcatcac aactgtctgg
      481 agtaaaatag aaggaagatc ttttagtttc agtgtacgat aaaacaccac agtcagtagc
      541 agcgtataga caacagcaat ggccgaggct tcaatagccg taaaaattcc ctgcacgatg
      601 ccaccgacaa taataacaat taacatcagg cttggaattg cttcaaccga gaccttcagc
      661 accatccctt tttgtacggt aaaaaaaaca cgatagttgt gccgtttcgc tacaatcagc
      721 gttaccagca tacatcctat gccccaaagt aagccggcaa ctaaccctcc agcgaaaagt
      781 gcagcaatag atgtcccacc actggccaat gcataaagaa taaaagccgt cgtgggcgga
      841 attaacatgc ctgttggtgc agaggcaata tttaccgcag cagcaaaact acgatcgtaa
      901 ccttcacgag cactcattgg taccataacg ccaccaatag aagttgaagc ggcaatcgcg
      961 gacccggaaa tagcgccaaa catcatattg ccaacaatat tcgtgtagga aagagaaccg
     1021 ggtaatttcc ccgtaaacag cttggcaaaa tttaccaggc gtgaagcgat accaccacta
     1081 ttcataatca cgccagaaag aacgaaaaac ggtaccgcca ataaagcaaa actgtccagg
     1141 ctggagaaca ttttttgtgc ggtagaaaac attgcgacgt cgaaaggtaa taccagcatc
     1201 attgtactga aagaggcaat aacgatgcat atcccgattg gtgagcccat tgcgagtaaa
     1261 acaacaaaaa cggcaattaa tatgcaagaa gcaaggatag gatcagtcat gtgatttctc
     1321 taaagaatgt aaagtagcat ccgttgttga acttttatct tttagtgact caataatatt
     1381 taacaaacta tagataataa ttagcactcc agaaacaggg agtgcgtaat aaatcttgga
     1441 cattgaaatg cctagggcag gagatacctg taatgcggaa accaccgata tccttaagcc
     1501 tccgataatt aatacccaaa cagaaaaggc aataaaaata atctgtagaa taatctccca
     1561 ccacctgcgt aatgttgtgg gagctttatc cagtaatatt gttaaactaa tatgctgttt
     1621 tttcccaaca acgaaagcca ttcctagcat agatacccat accagtgaga atcgcagcat
     1681 ttcttctgta agggtactag gtacatccag gatgtaacgg ctaataactt gccagcaagc
     1741 aataccgacc ataatagcca gcattaaaca acaaacgctg ccgagaagta aatcaagtcc
     1801 ttttctgaaa gtattcatta tcatttctgg cctggattaa tgttttgctg attgtatctg
     1861 ctgatatagt ttataaagct gaggtttgtt cttaagattg tcataaatag gctgaaccgc
     1921 atcctggaaa ggttttttat caatctcgaa aaactcgacg ttaaagtctt ttactgctgc
     1981 ctgttttgtt ttttcaattt ctttatccca tgctgctttt tcaaattcga tggaagcgcg
     2041 gatggcatct ttaacaatgg tacgctgttc aggcgtcagt tttgctaatg ttgcagtgct
     2101 cattagtaaa acgtcaggaa tacgggtgtg catatcataa gtatagtaac gtgcaacctc
     2161 accatgacgg gcaatcgtta aagcaaattc gttattttct gctccatcaa gaataccttg
     2221 ctgaagggag gtataaactt cagcttgact cattgcaatg ggagatgctc ccaggagttt
     2281 aagtgtctga atagacgtgt cgctctgcat gacgcggatt ttttttccgc gtaaatcttc
     2341 aatacttttg attggtgatt tactcatata aaagttacgt gcgccggaat cataccaacc
     2401 gatacctata aatccttgcg ctgccgttga ctgatacact gggtccatta tttttgggtt
     2461 atccattact ttataatatt catcggcatt actaaaaaga tacggtaagg aaaaaatacc
     2521 gtacagagga gagaaacttt ccatcagccc tgatgaaact tttgtgatat ctactgcatt
     2581 ggcttgtatt agttcaacca gttcgcgctc accaccgagt tgaccatcag gaaagtaagc
     2641 aacttttatt tcacccccgg ttttctcttc tacttgttga ccgaaaaagt gcagagcatc
     2701 tttaactggc tgactgtttt cggcataagc cagtcggagt gttgtttcgc ctctggagaa
     2761 gaaagaaacc gtaaaaagca aagttagcat tactgtgcgt ataaacagag atcgttccat
     2821 gataattcct tgtaagtgta gtaattgaga tgaatataat tgagccgtgg aattataacc
     2881 ttttttattt tgccactttt gatgaaccct ggcattaatg agaaggtttg gaagtcacat
     2941 ttcacatttt tggccccacc cacctaatcg taggcacatg gctacatgag taagctttca
     3001 attaaaagtg attcatgacc aaaccagcac cagcgagaaa aagggcttga atgtcttata
     3061 tacgagcaac aaatgcggtg atttcatggg tgtggtaaaa taaatttgct gataactata
     3121 tgattagtaa agggattttt attgtgtctg agccgccatt ttcagatttt aacttttatt
     3181 cagatgttcg ctaatagggt ggtctacgac tgcctggtgc cggcgctaat gaagattttc
     3241 atgttgagta gtatgagctc ccctcatgag agaatcaaac accttcaatg attacgggga
     3301 tataaaacga tgttttttgt ttttttgcca tgttacaaag ataagtgtca ataataataa
     3361 ggtaatccgt gccagggtat tttattgtgg aaaggtaata ttgaatttta tgtgactaaa
     3421 atgttcgtgt ttagtgaaaa ttaatattta tgttatgaat gaaatgattc agatacatca
     3481 gaaaatatat acataacgta tctgttgggc gtaaatctaa ccatcttcag acctggtaat
     3541 gacgtaacga gatggctcaa catccgcgtt aattcgactt ataactgaga ttaataatat
     3601 gaaggtttgg cccgtaaaac atagtccatt attgcgtcaa ccagagcgtt taattacgca
     3661 gaatgagttg caggcactga tccgaaaaat aactaacaat ctggttaata taaaagatga
     3721 agcggggcaa tttcttctgc ggttagatga tggacgggtg attgatacaa aaggatggga
     3781 tggttgggaa tggacccacg gtgtgggttt atacggtatt tatcagtatt atcaacaaac
     3841 aggtgattct gtaatgtgcg atatcattga aaactggttt tcagatcgct ttgcagaagg
     3901 cgccacgacc aaaaatgtta atactatggc tccatttcta accctggctt atcgctacga
     3961 agacactggc agaccggagt atttgccctg gcttgatagt tgggctgagt gggctatgca
     4021 tactatgccg cgtaccaacc ataacggtat gcagcatatt accctggcgg aagagaacga
     4081 tcagcaaatg tgggatgaca cgttgatgat gaccgtcctg ccgctggcga aaatagggaa
     4141 attgctgaac cgtaatgaat atgttgaaga agctgtttat cagtttctgc tacacgtgca
     4201 aaatttacag gatagaacaa cgggcctgtg gtttcatggc tggagttacg atggatgcca
     4261 taactttgcc aatgccagat gggctcgtgg taacagctgg ctgacgattg tgatccctga
     4321 ctttttagaa ttgcttaatt tgccggaaaa caactcggta cgtcggtatc ttgttcaggt
     4381 attacatacg caaatagagg cgctggcaaa atgccaggat gagagcgggt tatggcatac
     4441 gttgctggac gatccaaatt catatctgga atcatcggct accgcaggtt ttgcctatgg
     4501 catcctgaag gcggtacgca agcattatgt tgcccccctg tatgccgagg tagccgagaa
     4561 ggcaattcgc gggatcgtga aaaatatttc tccggagggg gagcttttgc agacatcttt
     4621 cggtaccggg atgggagcaa atcttgattt ttatcgcgag atcccgctga catccatgcc
     4681 ctacggtcag gcaatggcag tgttgtgtct gacagaatat ctgcgtaaat atttttaatg
     4741 gacgtttcct gaccatacca ttctgaggtg gattgttaat ccgaagtcag gcaaaggtga
     4801 ccacaaggat ctttgcctgc gatactattt tttatcacag ccccggttta cctgcaccgc
     4861 aattgctcag gttacacagc cgaacgacaa cgagagtcgg taggctgact gaaaatacca
     4921 gattcagggg gtatatcggc gctatgccgc tctggcgcgg cggcgggaat ccattgaaat
     4981 caaaatcacc gatgacagaa tcagcagcgt tcccagccag tccggcaatg tgaaggtgat
     5041 ccccagcaac agcaaagaga gcagcgcgct acttaatggt tcagcacagc ttaaaatgct
     5101 ggcttttgga ccgccaatta attgtgctcc tttcaggtac agactaaatg tcagggacgt
     5161 accaatgacc accaaataaa aaaacgccag aatcaaactt ccgttaacga caaaatttgt
     5221 cccttgtccg gcataaaagg gaagcaagat cagaccgcca atgagcatac tccagccgac
     5281 gaccggtaac gtgccatagc gggcaatcag cattgaggga taggtggtat agaatgcagc
     5341 agcaaatgcc gaggcaatgc cccagaataa cgcggcagga gagatcgata atgacgtcgg
     5401 attaccgtgt gtcaccaata aaaaagtccc gatcagcgat gtcaaaatag cgcagaaaac
     5461 cagaatgccc gggcgggatt tacgcaccag tgagaaccag gcgacgataa tagtcggtga
     5521 gaggaattgc agcaccgttg ccgtggctgc gttcgatttt tcgatggtta gcaaaaaagt
     5581 gagctgtacg gttagcgcgc caaccacgga aaaaatcagt aggctaatgg catctttgtg
     5641 attgttaata atagaaaaga ttttatcacc atgaacaaat gatagcgtca gcagaatcaa
     5701 accggcgaat atcaaacgcg tcatagtcaa aaactgcgac gacatctggc tttgctccat
     5761 gatgtattgc gcgcagaccc ctgaacttcc ccacaacacg gcggcaatca gaacgttcag
     5821 catccccttt ctggtggaac ccatttttcc ctctgcatac tgttttgtcg tttatgtatg
     5881 caggcatgat agcaaaaatg gcgaggatgg ctcagtttgc gccacgccga tttttcttag
     5941 cccagactgg tgagctaaat atctttaata aacagcaagc aaatcattgc aatacccaac
     6001 atccctatcg ccagccagaa caacgtgtgg tagctccaga tttcaaccat aatttcgtca
     6061 acagagccgg cgataatcta gccgatgcgt gaggtatttg catataacgt tgttgcggaa
     6121 cctattctta tttatcggag tgccttgcca gtaatttact gacgaggatc ctcataatag
     6181 cgttacccat ctgttgcatt gagcctttac aggccgttat tctaaatgac caaaattaaa
     6241 tcagtgcata aggggaagta ttctgtagtg atgtctccct gagatgcagg ttccactgga
     6301 gaaaattatg agcagtttgt ttcagaatga aataaaggat aaatttaaaa ctggacttgc
     6361 atatgtgcgg gggccagaaa aaagcggatc ttccgctcaa aatgcaggta gcgaaggtgg
     6421 taaaaatgcg ccgggagaag cagaagattg tcccgcgatg tccgtttgag aaacacgagt
     6481 ttgattaaac gcgtgagcat gatgatatta gtagctgacc agagaaatta tatccgtcgt
     6541 aagcaataaa aatcagaagt gatgttatgg atatttttaa cctgaataac tccgtaagta
     6601 cggggttatt caggtttttg ctagcctgtg cttttctata ccagatgtgg tgtgaacact
     6661 ctattctgca tacaaattaa atatattgta ggatctaata taagtctgat aatcctaata
     6721 gactgagtaa gattctggtt gataatagct gatgtccttt tcagctatta atgtgtttat
     6781 ttagcgtgaa attatactcg tatttttata actttatgag attgccagat ttggtcgata
     6841 aataataagg ccagtgacag acttacttat tagggacaaa tttcgcgagg atatcaggtt
     6901 cactggcaac ataaatgctt tcatcaggca gagttacaat tccttcaggt tggggcaaat
     6961 cactggtcag agaatgattt ccttttgtca ggtcaagcat ctcaatgaaa gacatctctg
     7021 tcaggttaaa cttgagtaat ttacgcgatt catcggacag aatcatcaga ctttcattat
     7081 taaaggccag gccggatata tcgttaacac gtatatcatt tagtgcctct ggaatagttg
     7141 cttgctttac actcagcagg tctggagaaa gttgatatat aaatatttta cttgggcgtc
     7201 tttcttttgc gacaaacagc atccgatcat ctgctttcca tgccagccct tcaaaccctc
     7261 tattcttttt attgaatttt tctaactgaa ttttatttcg aaaggagata tgcatatctt
     7321 ttgtaactgt aaaaaagaat agttcagaag tcttttcgtc tactgcagca aaaatgttcc
     7381 cttgtatgtg ttcaattgtt tcagcatcag agataaaagg cagcggggct ctgtccatga
     7441 tgtcgccact tttggttatt ttaagtagct gtgcagggct attctgggtg gcatacagga
     7501 aatcatcctc tttattataa gtaagagatg at
//
LOCUS       DADSYM010000185         7342 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2415
            SAMN08039579-rid11723173.denovo.187, whole genome shotgun sequence.
ACCESSION   DADSYM010000185 DADSYM010000000
VERSION     DADSYM010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039579
            Sequence Read Archive: SRR6362533
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7342)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7342)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980162.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 03:39:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,484
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 4,347
            CDSs (with protein)               :: 4,347
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 43 of 85
            Pseudo Genes (incomplete)         :: 37 of 85
            Pseudo Genes (internal stop)      :: 19 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7342
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039579-rid11723173.denovo.187"
                     /strain="LGE2415"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8302 N 95.9628 W"
                     /collection_date="2015-08-24"
     gene            378..1292
                     /locus_tag="J1Y83_003951"
     CDS             378..1292
                     /locus_tag="J1Y83_003951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312409.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemin ABC transporter substrate-binding protein"
                     /protein_id="HBA7632052.1"
                     /translation="MPRIITRPFLFSPLTLCISAVVSAAKTMVKRKKLFTALLALSWT
                     FSVTAAERIVVAGGSLTELIYAMGAGKRVVGVDETTSYPPETAKLPHIGYWKQLSSEG
                     ILSLRPDSVITWQDAGPQIVLDQLRAQKVNVVTLPRVPATLEQMYANIRQLAKTLQVP
                     EQGEALVTQINQRLERVQQNVAAKKAPVKAMFILSAGGSAPQVAGKGSVADAILSLAG
                     AENVATHQQYKSYSAESLIAANPEVIVVTSQMVDGDINRLRSIAGITHTAAWKNQRII
                     TVDQNLILGMGPRIADVVESLHQQLWPQ"
     gene            1312..2649
                     /locus_tag="J1Y83_003952"
     CDS             1312..2649
                     /locus_tag="J1Y83_003952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme anaerobic degradation radical SAM
                     methyltransferase ChuW/HutW"
                     /protein_id="HBA7632053.1"
                     /translation="MNTNNTLDLTPHFALDGDQPFKDRRAMMPFRGAIPVAKEQLAQT
                     WQEMINQTVSPRKRLVYLHIPFCATHCTFCGFYQNRFNEDACAHYTDALIREIEMEAD
                     SVLHQSAPIHAVYFGGGTPSALSAHDLARIITTLREKLPLAPDCEITIEGRVLNFDAE
                     RIDACLDAGANRFSIGIQSFNSKIRKKMARTSDGPTAIAFMESLVKRDRAAVVCDLLF
                     GLPGQDAQTWGEDLAIARDIGLDGVDLYALNVLPNTPLGKAVENGRTTVPSPAERRDL
                     YLQGCDFMDDAGWRCISNSHWGRTTRERNLYNLLIKQGADCLAFGSGAGGSINGYSWM
                     NERNLQTWHESVAAGKKPLMMIMRNAERNAQWRHTLQSGIETARVPLDELTPHAEKLA
                     PLLAQWHQKGLSRDASTCLRLTNEGRFWASNILQSLNELIQVLNAPAIALEKP"
     gene            2662..3156
                     /gene="hutX"
                     /locus_tag="J1Y83_003953"
     CDS             2662..3156
                     /gene="hutX"
                     /locus_tag="J1Y83_003953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme utilization cystosolic carrier protein
                     HutX"
                     /protein_id="HBA7632054.1"
                     /translation="MSHVSLQEFLKTEPDGTLEAIAEQYNTTLLEVVKNLPSPTVVSG
                     DKFDTVWDTVCEWGNVTTLVHTTDVILEFSGELPSGFHRHGYFNLRGKHGMSGHIKAE
                     NCTHIALIERKFMGMDTASILFFNKEGSAMLKIFLGRDDHRQLLSEQVSAFHALAASL
                     KEHA"
     gene            3156..3779
                     /gene="chuY"
                     /locus_tag="J1Y83_003954"
     CDS             3156..3779
                     /gene="chuY"
                     /locus_tag="J1Y83_003954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobilin reductase"
                     /protein_id="HBA7632055.1"
                     /translation="MTPWLLFGAGGKGVGARTLELALAEQRPVVAVIRHADAATKLAQ
                     QGVQVFTGDACDASVVAAACRAAGPDALIISTMGGAQDYLAHRTVIDEAEKAGITRMI
                     LVTSLGCGDSWPFLSERAKAAFGQAVREKTLAESWLQTSQLDYAILRPGGLLDGAATG
                     KAQRIQNQECHGFVNRADVAAHIHELANAPALNQQVYSLIEPDLKPA"
     gene            3864..4820
                     /locus_tag="J1Y83_003955"
     CDS             3864..4820
                     /locus_tag="J1Y83_003955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HBA7632056.1"
                     /translation="MGLSLLLLALVLFGASQGALKISFDALFDEEYRDIWLNIRLPRV
                     LLAVLVGAALATAGVIMQGLFRNPMADPGLLGVSSGSALMVGVAIVLPFSFPVVLVLY
                     EQMVFAIAGSLVVCTIIFLITQRHRDGSMMQLLLAGIAINALCGAAIGILSYIGDEQQ
                     LRQLTLWMMGNLGQAQWPTLLVASSFILPAIIATTCLAGTLNLLQLGDEEAHYLGVNV
                     KRKRQQLLLVSSLLVGAAVSVSGIIGFIGLVIPHLIRMTTGANHRWLIPCSALAGACL
                     LLMADTLARTLVQPAEMPVGLLTSLLGGPYFMWLILRNRRIT"
     gene            4817..5587
                     /locus_tag="J1Y83_003956"
     CDS             4817..5587
                     /locus_tag="J1Y83_003956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC transporter ATP-binding protein"
                     /protein_id="HBA7632057.1"
                     /translation="MISAQNLVYSLQGRRLTDNVSLTFPGGEIVAILGPNGAGKSTLL
                     RQLTGYLQPDSGECRLFNKPLNEWSITELAKHRAVMRQNSHMAFPFSVQEVIQMGRHP
                     HRTGNQDNETAQIMALCDCQALANRNYRQLSGGEQQRVQLARLLVQLWEPTPSPKWLF
                     LDEPTSALDIHHQQHLFRLLRQLVHERQFNVCCVLHDLNLAARYADRVVLMQKGKVIA
                     NGKPQDVLTQQALTMLYGADITVLKDPANHSPLIVLDH"
     gene            complement(5639..6286)
                     /locus_tag="J1Y83_003957"
     CDS             complement(5639..6286)
                     /locus_tag="J1Y83_003957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HBA7632058.1"
                     /translation="MTAEFIIRLILAVIVCGAIGMERQMRGKGAGLRTHVLIGMGSAL
                     FMIVSKYGFADVLSLGHVGLDPSRIAAQVVTGVGFIGAGNILVRNQNIVGLTTAADIW
                     VTAAIGMVIGSGMYELGIYGSVMTLLVLEVFHQLTFRLMNKNYHLQLTLVNGNTVSML
                     DWFKQQKIKTDLVSLQENEDHEVVAIDIQLHATTSIEDLLRLLKGMAGVKGVSIS"
     gene            complement(6350..6676)
                     /gene="hdeB"
                     /locus_tag="J1Y83_003958"
     CDS             complement(6350..6676)
                     /gene="hdeB"
                     /locus_tag="J1Y83_003958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417966.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-activated periplasmic chaperone HdeB"
                     /protein_id="HBA7632059.1"
                     /translation="MNISSLRKAFIFMGAVAALSLVNAQSALAANESAKDMTCQEFID
                     LNPKAMTPVAWWMLHEETVYKGGDTVTLNETDLTQIPKVIEYCKKNPQKNLYTFKNQA
                     SNDLPN"
     gene            complement(6792..7124)
                     /gene="hdeA"
                     /locus_tag="J1Y83_003959"
     CDS             complement(6792..7124)
                     /gene="hdeA"
                     /locus_tag="J1Y83_003959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-activated periplasmic chaperone HdeA"
                     /protein_id="HBA7632060.1"
                     /translation="MKKVLGVILGGLLLLPVVSNAADAQKAADNKKPVNSWTCEDFLA
                     VDESFQPTAVGFAEALNNKDKPEDAVLDVQGIATVTPAIVQACTQDKKASFKDKVKGE
                     WDKIKKDM"
BASE COUNT         1869 a         1830 c         1755 g         1888 t
ORIGIN      
        1 atcgatcaat tttttatgat caagcttttt ttaaccgcca ttaacacaat aaacaggggg
       61 ttatgaaatc tatccagcat ccagaaaacc atcctgtgtt tatggctatc cccccacaac
      121 ctcatcacga aacgttgccg ttaacagtga taattttctc ttctctttgc tctgcaagcc
      181 gcgatatctc cgggttctca actttttccc gcgctgaaat ttaatcggta acaagaacat
      241 tagctgacga agcatcttga taaaaatcaa aaataaatac gcttgtaacg ccgtgcgtat
      301 tgaatcgacg gttgtatttc atatataact caaatgataa tcatgatcat tctcaatttg
      361 ataagagttc tcacattatg ccaaggatca tcaccaggcc gtttcttttt tccccactaa
      421 cgttatgtat ttcagccgtg gtctctgccg cgaagaccat ggtaaaacga aaaaaattgt
      481 ttacggcatt actggctctt agctggactt ttagcgtaac ggctgccgaa cgtatcgtgg
      541 tcgcaggagg atcgctgacg gagttaatct acgcgatggg cgctggcaaa cgcgtggttg
      601 gtgtcgatga aacgacatct tatccaccag aaaccgccaa actgccgcat attggttact
      661 ggaaacaact cagcagcgaa ggcattttgt cacttcgccc ggatagcgta attacctggc
      721 aggatgcagg accgcaaatt gtgctcgatc agctgcgggc gcaaaaagtc aacgtcgtca
      781 ctctgccgcg tgtaccagcc acccttgagc agatgtacgc caacattcgt caactagcaa
      841 aaacgttaca ggttcctgaa caaggcgagg cgctggtgac acagatcaac caacgcctgg
      901 agcgagtaca gcaaaacgtg gcggccaaaa aagccccggt taaagccatg tttattctct
      961 ccgcaggcgg aagtgcgcca caggttgccg gtaaaggcag cgtcgcggac gccattcttt
     1021 cgcttgccgg agcagaaaac gtcgctaccc accagcagta caaaagctac agcgcggaat
     1081 cgctgattgc ggctaaccct gaagtgattg tcgtcacctc acaaatggtc gatggcgata
     1141 ttaatcggct acgttctatt gccggaatta cccacactgc cgcatggaaa aaccagcgca
     1201 ttatcaccgt tgatcaaaac ctgattctgg gaatgggtcc gcgtattgct gatgtcgttg
     1261 agtctttaca ccagcagctt tggccgcaat aaatggattt aaatcagacg aatgaacaca
     1321 aataacaccc tggatctcac gccacatttt gctttagacg gtgatcagcc ctttaaagac
     1381 aggcgcgcca tgatgccgtt tcgtggggcc attccggttg ccaaagagca actggcacag
     1441 acctggcaag agatgatcaa tcaaacggta tcaccacgta agcgactggt ttacctacac
     1501 attccttttt gtgcgacaca ctgcacgttt tgtggttttt atcagaaccg ttttaatgaa
     1561 gatgcgtgtg ctcattatac cgacgccctg attcgtgaaa tagaaatgga agcagacagc
     1621 gtattgcatc agtcggcccc catccacgcg gtctatttcg gtggcggcac gccttccgcg
     1681 ctttcagcac acgatttggc cagaatcatc accacattac gagaaaagct gccactggcc
     1741 cctgattgtg aaatcactat cgaaggccgg gtactgaatt ttgacgctga gcgaatcgat
     1801 gcttgccttg atgcaggagc aaaccgcttc tcaattggta ttcagtcgtt taacagcaaa
     1861 atccgcaaga aaatggcccg cacctcggat ggcccaaccg ctattgcgtt tatggaaagc
     1921 ctggtcaaac gcgaccgtgc cgcggtggtc tgtgacctgc tgtttggtct gccgggtcag
     1981 gatgcgcaaa cctggggaga agatctggct attgcccgcg atatcggtct cgacggcgtc
     2041 gatctctatg cgctcaatgt cctgcccaat acaccgctgg gcaaagccgt ggaaaatggg
     2101 cgtactaccg tgccctctcc ggcagaacgt cgcgatcttt acctgcaagg atgtgatttt
     2161 atggacgatg ccggttggcg ctgcatcagt aacagccact ggggccgtac cacacgcgaa
     2221 cgcaatctct ataacctgct gattaaacaa ggtgccgatt gtctggcctt tggttccgga
     2281 gccggcggtt cgattaatgg gtactcctgg atgaacgaac gcaatcttca gacctggcat
     2341 gaatccgtcg cggcaggcaa aaaaccgctg atgatgatca tgcgtaacgc cgaacgtaat
     2401 gcgcaatggc gtcatacctt gcagtcgggc attgaaacgg cgcgtgtacc gctggacgaa
     2461 cttacgccac atgccgaaaa actcgcgccg ttactggccc agtggcacca aaaaggctta
     2521 agccgcgatg cgtcaacgtg tctgcggttg accaatgaag gtcgtttctg ggcaagcaac
     2581 attttgcagt ctcttaatga actgattcag gtacttaatg cgccagcaat tgcgcttgaa
     2641 aaaccataac aggagtgatt tatgagccat gtctcgttac aggaattttt gaaaacggag
     2701 ccagacggta cgctggaggc gatcgccgaa caatacaaca ccacgctgct ggaggtagtg
     2761 aaaaatctgc cctctccgac tgttgtgtca ggtgataaat tcgatactgt ctgggatacc
     2821 gtctgcgaat ggggcaacgt caccacgtta gtacatacta ccgatgtaat actcgaattt
     2881 agcggcgaac tgccttcggg tttccatcgc cacggctatt tcaacctacg cggaaagcac
     2941 ggcatgtccg ggcatatcaa agcagaaaac tgcacgcata ttgccttgat cgaacgtaaa
     3001 tttatgggca tggacaccgc ctctattctc tttttcaata aggaaggcag cgcaatgctg
     3061 aaaatcttcc tcggtaggga tgatcatcgt cagctcctga gcgaacaagt cagcgccttc
     3121 catgctctgg cagcatcgtt aaaggaacac gcctgatgac accgtggcta ctctttggcg
     3181 caggcggaaa aggtgtgggt gccagaacgc ttgaactggc gctggcggaa caacgtccgg
     3241 tcgttgctgt cattcgtcat gccgatgccg ccacgaaact ggcgcaacaa ggcgtacagg
     3301 tttttacagg cgacgcctgt gatgccagcg tagtcgcggc tgcttgccgc gcagctggcc
     3361 cggatgcgct tatcatctca acaatgggcg gagctcagga ttatctggcg cacagaacag
     3421 tgattgacga ggccgagaaa gcaggcatca cacgcatgat tctggtgacg tcactgggat
     3481 gcggcgatag ctggccgttt ttatcagagc gggcaaaggc tgcgtttggt caggcggtac
     3541 gggaaaaaac gctggcggaa agctggttgc aaaccagcca gcttgattat gccattctcc
     3601 gccccggcgg tctgcttgat ggtgcggcaa cgggcaaagc acaacggata caaaatcagg
     3661 agtgtcatgg ttttgttaac cgtgctgatg tcgccgcaca tatccatgaa ctggcaaatg
     3721 cgccagcact taaccagcag gtctacagtc tgattgaacc ggacctgaaa ccggcgtgat
     3781 cccccggcgc ggtatgtccg cgccgtaatg tcgtaacgga gttttgcgtg ctcaaggatt
     3841 cactctcttc cgccaggatc tttatggggc tatcactatt attgctagct ctggtgctgt
     3901 ttggtgccag tcagggagcg ttaaagatca gttttgatgc cctttttgat gaggaatacc
     3961 gcgatatctg gctcaatatt cgtctaccac gggttttgct ggcggtgctg gtaggtgcag
     4021 cgttggcaac cgcaggggta attatgcagg ggctttttcg caacccaatg gctgaccctg
     4081 gattacttgg cgtcagtagc ggttctgcat taatggttgg cgttgctatt gtgctgccct
     4141 tctccttccc agtggtgctg gtgctctatg agcaaatggt gtttgccatt gccggaagtt
     4201 tagtggtctg caccatcatt tttctcatca cgcagcgcca tcgcgatggc agcatgatgc
     4261 aattattact cgccggtatc gccatcaatg ccctgtgcgg cgcagcgatc ggcatcctga
     4321 gctatatcgg cgatgagcag cagctacgac aactcacatt gtggatgatg ggcaatcttg
     4381 gacaggcgca atggccgacg ttattggttg ccagttcatt catcctaccg gccattatcg
     4441 caacaacttg tctcgccgga acgctgaatt tactgcagct cggtgatgaa gaagcccact
     4501 acctcggcgt gaacgtgaag cgtaaacgcc agcaattact gttagtgagc tcactgctcg
     4561 ttggtgctgc cgtatcggta agcggcatta tcggctttat tggcctggtg atcccgcatc
     4621 tgattcgcat gactaccggg gcaaatcacc gctggctaat cccttgttcc gccctcgccg
     4681 gagcctgttt attgctgatg gcagacacgc ttgcccgcac gctggtacag ccagcagaaa
     4741 tgcccgtggg attattaacc agcctgcttg gtggccctta ttttatgtgg ttgattctgc
     4801 gcaaccggag gatcacatga tcagcgccca gaatctggtt tatagccttc aggggcggcg
     4861 tctgactgat aatgtctcgc tgacttttcc cggtggggaa attgttgcca tcctcggccc
     4921 taatggtgcg ggaaaatcta cgctgcttcg ccagctaacc ggctatcttc aacctgatag
     4981 cggcgaatgt cggttgttta ataagccatt aaatgaatgg tcgattactg aactggcgaa
     5041 acaccgggcc gtgatgcgtc aaaatagcca catggcgttt ccattcagcg tacaagaagt
     5101 tatccagatg gggcggcatc cgcatcgtac tggtaatcag gataatgaaa cagcacaaat
     5161 aatggcgtta tgtgactgtc aggcgttagc aaaccgcaac tatcggcaat tatccggtgg
     5221 tgaacaacag cgcgtgcaat tagcgcgcct gctggtgcaa ctctgggaac ctactccctc
     5281 accgaaatgg ttatttcttg atgaaccaac ttcggcgctg gatattcacc atcagcagca
     5341 cttgtttcgt ttattacgcc agttagttca cgaacggcag ttcaatgttt gctgcgtatt
     5401 gcacgatcta aatcttgccg cgcgttatgc agaccgtgtt gtattaatgc aaaaaggaaa
     5461 ggttatcgca aatggtaagc cgcaggacgt attgacacag caggcgctga caatgctcta
     5521 cggcgcggat attaccgtac ttaaagatcc tgccaaccat tcaccactga tcgtcctcga
     5581 tcattaaaac caacaggagt accgcactgg tactcctgcg aataaataac tatacagatt
     5641 agctaattga aacgcctttc acccctgcca taccttttaa taatcgcaac agatcttcta
     5701 tcgacgtcgt ggcatgaagc tggatatcaa tggcgaccac ttcatgatct tcattttcct
     5761 gcaacgaaac taaatccgtt tttatttttt gctgcttaaa ccagtcgagc attgataccg
     5821 tattaccgtt cactaatgtc agctgtaaat ggtaattttt gttcatcagg cggaaggtta
     5881 attgatggaa gacttccagc accagcaagg tcatcactga accataaata cccagttcgt
     5941 acataccgct gccaataacc ataccaatgg cggcggtcac ccagatatcc gctgccgtcg
     6001 tcagacctac aatattttgg ttacgaacga gaatgttacc tgcaccgata aacccgacgc
     6061 ccgtcacaac ctgagcagca atacggctgg gatccagtcc gacgtgacct aaagatagca
     6121 cgtcagcaaa accatatttc gaaacaatca taaacagggc gcttcccatg ccaattaata
     6181 catgtgtgcg taaccctgct cctttgccgc gcatttgcct ttccatgcca atagcgccac
     6241 agacaattac cgcaagtata agtcgtataa taaattctgc tgtcatataa aaatgcaaaa
     6301 aggagcagca agatggctca acttgctact cctttttact tgtaccttat taattcggca
     6361 agtcattaga tgcttgattt ttgaaggtat acaaattttt ctgcgggttt ttcttgcagt
     6421 attcgatcac tttaggaatt tgagtgagat cggtttcatt taaagtaacg gtatcgccac
     6481 ctttatatac tgtttcttca tgcagcatcc accacgcaac tggtgtcatt gcttttggat
     6541 tcagatcaat aaattcctgg caggtcatat ctttagcgga ttcattggct gccagcgcag
     6601 attgtgcgtt caccagtgac aaagccgcta cagcgcccat aaaaataaac gctttacgga
     6661 gagatgaaat gttcattttg taacccattc aatatagaga ttatatacaa acaggaggaa
     6721 tagctgcttt taaagaagat attccggcct gttcaatatg taaggaaata cgatgtaacg
     6781 cattcccgga attacatatc tttcttaatt ttgtcccatt cgcctttaac tttatcttta
     6841 aagctggctt ttttatcctg agtacaagcc tgaacgatag ctggggttac ggttgcaata
     6901 ccctgaacat ctaaaaccgc atcttctggt ttatctttgt tgttcagtgc ttcagcaaaa
     6961 ccaactgcag ttggctggaa ggattcgtcc acagccagga aatcttcaca ggtccaggag
     7021 ttgaccggtt ttttgttatc agctgctttt tgtgcatccg ctgcattgct cactactggc
     7081 agaagaagca gaccaccaag aataacgcct aatacttttt tcatcgtaat atcctcaact
     7141 ataaagtgaa agagccgtca cgaatcaatt tcgacactga ggttataacc tggttttctg
     7201 tatatgtcat gttgatggaa aatatcaaaa tcagatattt ttatttcaat acaatgagtt
     7261 acagacgcat cagatactgt aattaggaaa tttttattaa atcgactgca ttcttacacg
     7321 cgtttttggc atagattgat ag
//
LOCUS       DADSYM010000186         7036 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2415
            SAMN08039579-rid11723173.denovo.188, whole genome shotgun sequence.
ACCESSION   DADSYM010000186 DADSYM010000000
VERSION     DADSYM010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039579
            Sequence Read Archive: SRR6362533
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7036)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7036)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980162.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 03:39:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,484
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 4,347
            CDSs (with protein)               :: 4,347
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 43 of 85
            Pseudo Genes (incomplete)         :: 37 of 85
            Pseudo Genes (internal stop)      :: 19 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7036
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039579-rid11723173.denovo.188"
                     /strain="LGE2415"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8302 N 95.9628 W"
                     /collection_date="2015-08-24"
     gene            113..2458
                     /locus_tag="J1Y83_003960"
     CDS             113..2458
                     /locus_tag="J1Y83_003960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012907487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent DNA methyltransferase"
                     /protein_id="HBA7632061.1"
                     /translation="MSSTNFSQIAAFLWSVADLLRGDFKQSQYGRIILPFTLLRRLEC
                     VLETTKDAVIAEAQKVKAMKLPEEAQEKMILRATNGLTFFNASAMDLSKMGQNGIQDN
                     LENYIQSFSSDAREIFEHFKFSEFVGQLADANLLFKVVQIFAKADLYPEHVTNHDMGL
                     VFEELIRRFAESSNETAGEHFTPRDIVNLTTSLVFFDDDDALNKDGIIRTIYDPTAGT
                     GGFLSSGMEFVHQQNPNAVMRAFGQELNPESYAICKADMLIKGQDVSLIKLGNTLSND
                     QLPAEKFDYMLSNPPFGVDWKKIETDINDEHKLKGADGRFGPGLPRVSDGSLLFLLHL
                     ISKMRDAKSGGGRIGIILNGSPLFTGGAGSGESEMRRYILEADLLEGIIALPTDMFYN
                     TGIATYVWVLSNKKAPERKGKVQLIDGSNLCGKMRKSLGSKRNILGEEDIGLITRTFG
                     DFEPVATTTLAALGLEKAPEQKSSRGRQPATTKTEAAKTFASKVFHSTEFGYRRITVE
                     RPLRLSAQISDDAIATLRFAPKPFNAPMEQLYDAFAFHWQDGNYGDLTAVESEARAIL
                     KADFSELKEKQIKDLLDSKLWLAQRGLMEKARRIQAAMGMQAGGKDTVSNDFNQFQLT
                     LKEALRTAGVKLDAKENKQFIDAITRKNPDAEPVISKVLKEAAQPLYGAFEYHGKVVE
                     FESDGDLRDNENVPLNPAVSTSELIEGYFKAEALPHVADAWINADKRDAKDGDIGIVG
                     YEIPFNRHFYIYQPPRPLEEIDADLDAISAEIMKLLQEVHS"
     gene            2458..3741
                     /locus_tag="J1Y83_003961"
     CDS             2458..3741
                     /locus_tag="J1Y83_003961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HBA7632062.1"
                     /translation="MAGLNKYQAYPEYRDSGVEWLGEIPRTWQAYTGKRLFSSVRTPA
                     LSNDEQLAASQKYGVIPQTLMMQLNDSKVMLALKGTDSFRHVEKNNFVISLRSFEGGI
                     EHSNYQGCVSPAYTVLKSVNDISFSFYRYLLKSEPYISALQASTDSLRDGKSINYEQF
                     GAITLPSPSLPEQSIIAHFLDHETAKIDNLIEKQQLIELLKEKRQAVISHAVTKGLNP
                     DVPMKDSGVEWLDEIPEHWRICKLKNLAKICNGQDYKLVQAETGYPVIGSGGQFAWAN
                     TFLYDKPSVLLGRKGTIDKPLYIDKAFWTVDTMYYTEVFDCIIEKYLYYLALTIQFNR
                     YATNTALPSMTQEHLGNYSFSLSFNLSEQKLIINSIEKSLIKIDALTEKQLKQIELLK
                     ERRTALISAAVTGKIDLRNWTAPIAKTEAPTEASA"
     gene            3738..7001
                     /locus_tag="J1Y83_003962"
     CDS             3738..7001
                     /locus_tag="J1Y83_003962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HBA7632063.1"
                     /translation="MSNDITKELNLQNDIIAQMVEKGWIVGKGDTYDRERALYAQDTL
                     NFVQTTQPQEWEKFARVYPNDTERHFLDALVAQLKKADSNATDIQSRTRGTLGVLRHG
                     LKIRNARFSLCQFKPEHNLNPETLARYQQNICRIVPELVYSPYASNSVFAETGVKAKK
                     WRIDLVLFINGLPIVTLELKSEFKQAVQNAIAQYKKTRLPKDPATNKPEPLLTFKRGA
                     LVHFAVSQYDVFMTTKLAGDSTFFLPFNKGTKDGGAGNEIPEDTHRYATDYLWNEVLL
                     PDNLLKILASFVHLQIEEKEDWNGLKAKKESLIFPRYHQWDVVNKLLEAATVEGTGHK
                     YLIQHSAGSGKSNSIAWTAHQLSRLYDVNGEKQFHSVIVVTDRTVLDDQLQDTIWQFE
                     HADGVVGRINNREGDGSKSEKLANALENSQPIIIVTIQTFPFVLKAIENSVSLKQRKY
                     AVIADEAHSSQSGSTARQLKEVLMSEEMDDDLVLSSEDILDATIAARKGSSNLNYYAF
                     TATPKSKTLELFGRRPRPDEPASKTNKPEAFHVYSMRQAIEEGFILDVLKNYTNYKVA
                     YQLLQKLEDKDREVDSKRAKIKLNQWVILHDHNISQKVKVIVEHFRKHVMHLLGGQAK
                     AMVVTSSRKAAVRYKLAFDKYISENNYQKISAMVAFSGEVEFQEGDPNSPALLNQKFT
                     EINMNPGLKGRDMRKAFDSDDYQVMLVANKFQTGFDQPKLCAMYVDKALGGVECVQTL
                     SRLNRTYPGKAESGTFVLDFYNDPDEILGAFQPYFQTAELTDVSDPQQVFDLFEKLRA
                     TQIFQWHEVEQFCKAYFIKNKSNAAISNICKPAVERWKKRYLMAIDAYLTAKEMFERT
                     KKTGDTVLITNAENSFKACKQEKDRLEIFKKDLGSFVRFYEFMSQIVEYDDKDLEKLS
                     LFARHLRPLLHEQRLEEDEIDLSNVEMSHYRLSKIHEQDLRLQEETPDYTLDASNNIG
                     TAKAKDKKEEFLSQVLSRMNELFISDHLTDKDMISNAFAVYNKITENENVMTQIKNNP
                     PEQAILGDFLQAVDNAVMDSNDARQEFMLQYLSDPARAKSFAYLMFDMLTGKIALPT"
BASE COUNT         2021 a         1710 c         1718 g         1587 t
ORIGIN      
        1 taacgcattg aaaatgatca tgtttttgat catcacaacg atagtttgat ataacaaagc
       61 attcacttca cattgaatat ctccctcttt ttcagggcaa ggaatgcttt ttatgtcatc
      121 aactaatttc agccagattg cagccttcct gtggtctgtc gccgacctgt tacgcggcga
      181 ttttaaacaa tcacaatatg ggcgaattat tctccctttc actcttcttc gtcgtctgga
      241 atgcgttctc gaaacgacga aagacgcggt cattgccgag gctcaaaaag taaaagcaat
      301 gaaactgccg gaggaagccc aggagaaaat gatcctgcga gcgaccaatg gcctcacgtt
      361 cttcaatgcc tcagcgatgg atctcagcaa aatggggcaa aatggcatac aggacaacct
      421 ggaaaactac atccagtcgt tctcttcgga tgcccgcgaa attttcgaac acttcaagtt
      481 cagcgagttt gtcggacagc tggcggatgc gaacttactg ttcaaagtgg tgcaaatatt
      541 cgctaaagca gatttatacc cggagcacgt caccaaccac gatatggggc tggtatttga
      601 ggagctgatc cgacgttttg ccgagagttc taacgaaacc gcaggggagc actttacccc
      661 gcgtgatatc gtcaatctga ccacctcgct ggtgtttttc gatgatgatg acgcgctcaa
      721 taaagacggc atcatccgca ccatttacga cccaacagcg ggaaccggcg gctttctctc
      781 ctccggcatg gagttcgtcc accaacaaaa ccccaatgcc gtgatgcgcg cgtttggtca
      841 ggagttaaat ccggaatcct acgccatctg caaagccgac atgctgatta agggccagga
      901 tgtcagcctt atcaaactcg gcaacaccct gtccaacgat cagctccccg cagaaaaatt
      961 cgattacatg ctttctaatc cacctttcgg cgtggactgg aaaaagatag aaaccgacat
     1021 caacgacgag cacaaactca aaggtgctga tggtcgcttt ggccccggcc tgccccgagt
     1081 ctccgacggt tcgttgctgt ttctgctgca tctgatcagc aaaatgcgcg atgccaaaag
     1141 cggtggcgga cggatcggca tcattcttaa tggttcgccg ttattcaccg gcggcgcagg
     1201 cagcggtgaa agcgagatgc gccgctacat tctggaagcg gatttgctgg aaggcattat
     1261 cgccctgccc accgatatgt tttacaacac cggtatcgct acctatgttt gggtgctgtc
     1321 taacaaaaaa gcgccagaac gcaaaggtaa agtccagcta atcgacggca gcaacctgtg
     1381 cggcaagatg cgtaaatcgc tgggatcaaa acgtaatatt ctcggggagg aggatattgg
     1441 tttaatcacc cgcacgtttg gcgatttcga acccgttgcc accactacgc ttgcggcgtt
     1501 aggcctggag aaagcacccg aacaaaaatc cagccgggga cgccagcccg caacaaccaa
     1561 aaccgaggcg gcaaaaacct ttgccagcaa agtctttcac agcaccgagt ttggctatcg
     1621 tcgcatcacc gttgagcgcc cgttacgcct gtcggctcag ataagtgatg acgccattgc
     1681 cacattgcgc tttgcgccga agccgtttaa cgcgccgatg gagcaactgt atgacgcctt
     1741 tgccttccac tggcaggacg gtaattacgg cgacttaaca gcggtagaaa gtgaagcccg
     1801 cgccattctt aaagccgatt ttagcgagct gaaagagaag caaattaaag atctgctcga
     1861 cagcaaatta tggctggccc agcgcgggtt aatggaaaaa gcgcggcgta ttcaggcggc
     1921 gatgggtatg caggcgggcg gcaaagacac cgttagcaat gactttaatc agttccagtt
     1981 gacgctgaaa gaagcgctca gaaccgcagg cgtgaagctg gatgcgaaag agaacaagca
     2041 gtttatcgat gctatcaccc gcaaaaatcc ggatgccgag ccggttatca gtaaggtact
     2101 gaaagaagcg gcccaaccgc tgtacggcgc gtttgagtac cacggaaagg ttgtggagtt
     2161 tgagtcggat ggcgacctgc gcgataacga gaatgtgccg ctgaacccgg cggtatctac
     2221 cagcgagttg atcgaggggt acttcaaagc agaagcattg ccgcacgtcg cggacgcgtg
     2281 gattaacgcc gataagcgtg acgcgaaaga tggagatatc gggattgtgg ggtatgaaat
     2341 tccgtttaat cgtcattttt atatttatca gccgccaagg ccgctggagg agattgatgc
     2401 ggatctggat gcgataagtg ccgagattat gaaactgttg caggaggtac attcctgatg
     2461 gcggggttga ataagtatca ggcgtatccg gaatataggg attctggggt ggagtggttg
     2521 ggggagatac ctcgcacttg gcaggcgtac acaggaaaac gactatttag tagtgttaga
     2581 actccggctc tttctaatga tgaacaactt gctgccagtc aaaagtatgg cgttattcct
     2641 caaactttaa tgatgcaact taatgactct aaagtaatgt tggcattaaa aggtacagat
     2701 tcatttcggc atgtagaaaa aaataatttt gttataagtc tgcgtagctt tgaaggagga
     2761 attgagcaca gtaactatca aggttgcgtc tccccagcat acactgtgtt gaaaagtgtt
     2821 aatgacataa gcttttcttt ttatcgctat cttttaaaaa gtgagcctta tatatcagct
     2881 ttgcaagcgt caactgatag tttgagagat ggaaaatcga ttaattatga acaatttggt
     2941 gcaataacac tgccctcacc atctcttcca gagcagtcaa taattgctca ttttctcgac
     3001 cacgaaaccg caaaaatcga taacctaatc gagaaacaac aacttattga actactgaaa
     3061 gaaaaacgtc aggccgtgat tagccatgcg gtgaccaaag ggctaaatcc tgatgtgccg
     3121 atgaaagatt ctggggttga gtggttggac gaaatccctg aacactggag gatatgcaaa
     3181 ttaaaaaacc tagctaaaat atgtaatgga caggattata aactcgttca agcagaaacc
     3241 ggttatccag taataggctc cggggggcag tttgcatggg cgaatacttt tctatatgat
     3301 aaaccatcag tcctcttggg tagaaagggt acaattgata aaccattgta tatagacaag
     3361 gcattttgga ccgtcgatac aatgtattac acggaagtat ttgattgcat tatagagaaa
     3421 tatttatatt atttagcact gacaattcag tttaatcgtt atgcaactaa taccgcactg
     3481 cctagtatga ctcaagaaca cttaggtaat tactcatttt cattgtcatt caacctcagt
     3541 gagcaaaaac taataattaa tagcattgaa aaatcattaa ttaaaataga tgccctaacc
     3601 gaaaaacagt taaaacaaat cgaactcctc aaagaacgcc gcaccgccct tatctccgcc
     3661 gccgtcaccg gcaaaataga tctccgcaac tggaccgccc ccatcgccaa aacggaagca
     3721 cccacggagg ccagcgcatg agcaacgaca ttacaaaaga gctcaacctg caaaacgaca
     3781 tcatcgccca gatggtagaa aaaggctgga ttgtcggcaa aggtgacacc tacgacagag
     3841 agcgtgcact atacgcacaa gacaccttaa acttcgtaca aaccacccaa ccgcaggagt
     3901 gggagaaatt cgcccgcgtt taccccaacg acaccgagcg ccatttcctc gacgcgctgg
     3961 tcgcgcagct taaaaaagcc gacagcaacg caaccgatat ccagtcgcgc accaggggta
     4021 cgctcggcgt acttcgtcac ggcctgaaaa tccgcaatgc ccgtttttcg ctgtgtcagt
     4081 ttaaaccgga acataatctc aacccggaaa cgctggcgcg ctatcagcag aatatctgcc
     4141 gtattgtgcc ggaactggtc tacagcccgt atgcctcaaa tagcgtcttt gcagaaacgg
     4201 gcgtgaaagc caaaaaatgg cgcatcgacc tggtgctgtt tatcaacggc ctgccaattg
     4261 tcacgctgga gctgaaatcc gaattcaaac aggcggtgca gaacgccatt gcgcagtaca
     4321 aaaagacccg cctgccgaaa gatcccgcca ccaataaacc ggaaccgctg ctgaccttta
     4381 agcgcggcgc gctggtgcat tttgccgtca gccagtatga cgtctttatg accaccaaac
     4441 tcgcgggtga cagcaccttc ttcctgccgt ttaacaaagg caccaaagac ggaggcgcag
     4501 gcaacgagat ccccgaagat acccaccgct acgccaccga ttatctgtgg aatgaagtgc
     4561 tactgccgga caacttatta aaaatcctcg ccagttttgt ccatttgcag attgaagaaa
     4621 aagaggactg gaacgggctg aaagcgaaga aagagagcct gattttcccg cgttatcacc
     4681 agtgggatgt ggtcaacaaa ctgctggagg ccgcaacggt cgaaggcacc ggccataaat
     4741 acctgatcca gcacagcgcc ggttccggta aatcgaactc cattgcctgg accgcccacc
     4801 agctttctcg tctgtacgac gtgaacgggg aaaaacagtt ccactccgtt atcgtggtga
     4861 cggaccgcac ggtgctggac gaccagcttc aggacaccat ctggcagttc gaacatgccg
     4921 acggcgtggt cgggcgcatc aataacagag agggcgacgg ctctaaatcg gaaaagctcg
     4981 ccaacgcgct ggaaaactcg cagccgataa tcatcgtcac catccagaca ttcccgttcg
     5041 tgctgaaagc catcgaaaac agcgtcagcc tgaagcagcg taaatacgcg gtgattgccg
     5101 acgaagcaca ctcctcgcag agcggttcta ccgcccgtca gcttaaagaa gtgctgatga
     5161 gcgaagaaat ggatgatgac ttggtactgt cgtcagaaga tatcctggat gcgaccattg
     5221 ccgcccggaa aggcagcagt aacctgaact actacgcctt taccgccacg ccaaaatcca
     5281 aaaccctcga actttttggt cgccgtccac gtcccgatga accggcgtca aaaactaata
     5341 aacctgaggc cttccacgtc tactccatgc gccaggcaat cgaagaaggc tttatcctcg
     5401 acgtgctgaa aaactacacc aactacaaag tggcgtatca gttgctgcaa aagttggaag
     5461 ataaagaccg cgaagtcgat agcaaacggg cgaagataaa gctcaaccag tgggtgatac
     5521 tgcacgacca caatatctct cagaaggtga aggtgattgt ggagcacttc cgcaaacatg
     5581 tgatgcacct gcttggcggc caggccaaag cgatggtggt caccagttca cgcaaagccg
     5641 ctgtgcgcta caagctggcg tttgataaat acattagcga gaacaattac cagaaaatca
     5701 gcgcgatggt ggcgttttcc ggggaagttg aattccagga aggcgaccct aacagcccgg
     5761 cattgttaaa ccagaaattt actgaaataa acatgaaccc cggcctgaaa gggcgggata
     5821 tgcgcaaagc cttcgacagc gatgattatc aggtcatgct ggttgccaat aaattccaga
     5881 ccggttttga ccagccgaag ctgtgcgcga tgtacgtgga taaagccctt ggcggcgtcg
     5941 agtgcgtgca gacgctctcc cgcctgaatc gcacctatcc gggcaaagcg gaatcaggca
     6001 cttttgtcct cgatttttat aacgatccag acgagatcct cggcgcattc cagccctatt
     6061 tccagacggc ggaactgacc gatgtcagcg atccgcagca ggtattcgat ctgtttgaaa
     6121 aactgcgcgc cacgcagatt tttcagtggc atgaagtcga gcagttctgc aaggcgtact
     6181 tcatcaaaaa taaatcgaat gcggcgatca gtaatatctg taaaccggcg gtcgaacgct
     6241 ggaaaaagcg ttatctgatg gcaatagacg cctatttgac cgccaaagag atgttcgagc
     6301 gcactaaaaa aacgggcgat acggtgttaa tcaccaatgc ggaaaacagt tttaaagcct
     6361 gtaagcagga gaaagatcgt ctggagattt tcaagaaaga tctcggcagc tttgttcgct
     6421 tttatgagtt tatgtcgcaa atcgttgagt acgatgacaa ggatctggaa aagctaagcc
     6481 tgtttgcgcg ccatttacgc ccactgctgc atgagcaacg tctggaagag gatgaaattg
     6541 atctcagtaa tgtggagatg agtcactatc gtttatcgaa aatccatgag caagatttac
     6601 gcttacagga agaaacgcct gactatacgc tggatgcaag taataatatt ggtacggcaa
     6661 aagcgaaaga taaaaaagag gagtttttat ctcaggttct ttctcggatg aatgaattat
     6721 tcattagtga ccatttgacc gacaaagata tgatcagcaa tgcttttgcg gtgtataaca
     6781 aaataactga aaatgaaaat gttatgacac aaattaaaaa caatccgcca gaacaagcga
     6841 ttcttggtga tttcctccag gcagttgata atgctgtaat ggacagtaat gacgctcggc
     6901 aagaatttat gttgcaatat ttatccgatc cagctcgggc aaagagcttt gcttatctga
     6961 tgtttgatat gctaacgggg aaaattgctt taccaacata aaaatacgtt gatagccggg
     7021 gtcgcacttt ctaccc
//
LOCUS       DADSYM010000187         6999 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2415
            SAMN08039579-rid11723173.denovo.189, whole genome shotgun sequence.
ACCESSION   DADSYM010000187 DADSYM010000000
VERSION     DADSYM010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039579
            Sequence Read Archive: SRR6362533
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6999)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6999)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980162.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 03:39:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,484
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 4,347
            CDSs (with protein)               :: 4,347
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 43 of 85
            Pseudo Genes (incomplete)         :: 37 of 85
            Pseudo Genes (internal stop)      :: 19 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6999
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039579-rid11723173.denovo.189"
                     /strain="LGE2415"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8302 N 95.9628 W"
                     /collection_date="2015-08-24"
     gene            37..534
                     /gene="ftnA"
                     /locus_tag="J1Y83_003963"
     CDS             37..534
                     /gene="ftnA"
                     /locus_tag="J1Y83_003963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme ferritin"
                     /protein_id="HBA7632064.1"
                     /translation="MLKPEMIEKLNEQMNLELYSSLLYQQMSAWCSYHTFEGAAAFLR
                     RHAQEEMTHMQRLFDYLTDTGNLPRINTVESPFAEYSSLDELFQETYKHEQLITQKIN
                     ELAHAAMTNQDYPTFNFLQWYVSEQHEEEKLFKSIIDKLSLAGKSGEGLYFIDKELST
                     LDTQN"
     gene            complement(572..811)
                     /locus_tag="J1Y83_003964"
     CDS             complement(572..811)
                     /locus_tag="J1Y83_003964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YecH family protein"
                     /protein_id="HBA7632065.1"
                     /translation="MDSIHGHEVLNMMIESGEQYTHASLEAAIKARFGEQARFHTCSA
                     EGMTAGELVAFLAAKGKFIPSEEGFSTDQSKICRH"
     gene            1002..2213
                     /gene="tyrP"
                     /locus_tag="J1Y83_003965"
     CDS             1002..2213
                     /gene="tyrP"
                     /locus_tag="J1Y83_003965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine transporter TyrP"
                     /protein_id="HBA7632066.1"
                     /translation="MKNRTLGSVFIVAGTTIGAGMLAMPLAAAGVGFSVTLILLIGLW
                     ALMCYTALLLLEVYQHVPADTGLGTLAKRYLGRYGQWLTGFSMMFLMYALTAAYISGA
                     GELLASSISDWTGISMSATAGVLLFTFVAGGVVCVGTSLVDLFNRFLFSAKIIFLVVM
                     LVLLLPHIHKVNLLTLPLQQGLALSAIPVIFTSFGFHGSVPSIVSYMDGNIRKLRWVF
                     IIGSAIPLVAYIFWQLATLGSIDSTTFMGLLANHAGLNGLLQALREMVASPHVELAVH
                     LFADLALATSFLGVALGLFDYLADLFQRSNTVGGRLQTGAITFLPPLAFALFYPRGFV
                     MALGYAGVALAVLALIIPSLLTWQSRKHNPQAGYRVKGGRPALVVVFLCGIAVIGVQF
                     LIAAGLLPEVG"
     gene            complement(2276..2941)
                     /gene="yecA"
                     /locus_tag="J1Y83_003966"
     CDS             complement(2276..2941)
                     /gene="yecA"
                     /locus_tag="J1Y83_003966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein YecA"
                     /protein_id="HBA7632067.1"
                     /translation="MKTGPLNESELEWLDDILTKYNTDHAILDVAELDGLLTAVLSSP
                     QEIEPAQWLVAVWGGADYVPRWASEKEMTRFMNLAFQHMADTAERLNEFPEQFEPLFG
                     LREVDGSELTIVEEWCFGYMRGVALSDWSTLPDSLKPALDAIALHGTEENFERVEKMS
                     PEAFEESVDAIRLAALDLHAYWMAHPQEKAVQQPIKAEEKPGRNDPCPCGSGKKFKQC
                     CLH"
     gene            complement(3137..3223)
                     /locus_tag="J1Y83_003967"
     tRNA            complement(3137..3223)
                     /locus_tag="J1Y83_003967"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3187..3189),aa:Leu,seq:taa)
     gene            complement(3236..3309)
                     /locus_tag="J1Y83_003968"
     tRNA            complement(3236..3309)
                     /locus_tag="J1Y83_003968"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3275..3277),aa:Cys,seq:gca)
     gene            complement(3364..3439)
                     /locus_tag="J1Y83_003969"
     tRNA            complement(3364..3439)
                     /locus_tag="J1Y83_003969"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3404..3406),aa:Gly,seq:gcc)
     gene            complement(3591..4139)
                     /gene="pgsA"
                     /locus_tag="J1Y83_003970"
     CDS             complement(3591..4139)
                     /gene="pgsA"
                     /locus_tag="J1Y83_003970"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HBA7632068.1"
                     /translation="MQFNIPTLLTLFRVILIPFFVLVFYLPVTWSPFAAALIFCVAAV
                     TDWFDGFLARRWNQSTRFGAFLDPVADKVLVAIAMVLVTEHYHSWWVTLPAATMIARE
                     IIISALREWMAELGKRSSVAVSWIGKVKTTAQMVALAWLLWRPNIWVEYAGIALFFVA
                     AVLTLWSMLQYLSAARADLLDQ"
     gene            complement(4196..6028)
                     /gene="uvrC"
                     /locus_tag="J1Y83_003971"
     CDS             complement(4196..6028)
                     /gene="uvrC"
                     /locus_tag="J1Y83_003971"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HBA7632069.1"
                     /translation="MSDQFDAKAFLKTVTSQPGVYRMYDAGGTVIYVGKAKDLKKRLS
                     SYFRSNLASRKTEALVAQIQQIDVTVTHTETEALLLEHNYIKLYQPRYNVLLRDDKSY
                     PFIFLSGDTHPRLAMHRGAKHAKGEYFGPFPNGYAVRETLALLQKIFPIRQCENSVYR
                     NRSRPCLQYQIGRCLGPCVEGLVSEEEYAQQVEYVRLFLSGKDDQVLTQLISRMETAS
                     QNLEFEEAARIRDQIQAVRRVTEKQFVSNTGDDLDVIGVAFDAGMACVHVLFIRQGKV
                     LGSRSYFPKVPGGTELSEVVETFVGQFYLQGSQMRTLPGEILLDFNLSDKTLLADSLS
                     ELAGRKINVQTKPRGDRARYLKLARTNAATALTSKLSQQSTVHQRLTALASVLKLPEV
                     KRMECFDISHTMGEQTVASCVVFDANGPLRAEYRRYNITGITPGDDYAAMNQVLRRRY
                     GKAIDDSKIPDVILIDGGKGQLAQAKNVFAELDVSWDKNHPLLLGVAKGADRKAGLET
                     LFFEPEGEGFSLPPDSPALHVIQHIRDESHDHAIGGHRKKRAKVKNTSSLETIEGVGP
                     KRRQMLLKYMGGLQGLRNASVEEIAKVPGISQGLAEKIFWSLKH"
     gene            complement(6025..6681)
                     /gene="uvrY"
                     /locus_tag="J1Y83_003972"
     CDS             complement(6025..6681)
                     /gene="uvrY"
                     /locus_tag="J1Y83_003972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UvrY/SirA/GacA family response regulator
                     transcription factor"
                     /protein_id="HBA7632070.1"
                     /translation="MINVLLVDDHELVRAGIRRILEDIKGIKVVGEASCGEDAVKWCR
                     ANAVDVVLMDMSMPGIGGLEATRKIARSTADVKIIMLTVHTENPLPAKVMQAGAAGYL
                     SKGAAPQEVVSAIRSVYSGQRYIASDIAQQMALSQIEPEKTESPFASLSERELQIMLM
                     ITKGQKVNEISEQLNLSPKTVNSYRYRMFSKLNIHGDVELTHLAIRHGLCNAETLSSQ
                     "
BASE COUNT         1712 a         1816 c         1641 g         1830 t
ORIGIN      
        1 ctacagcaac aaatataacc tttgtggagc actatcatgc tgaaaccaga aatgattgaa
       61 aaacttaatg agcagatgaa cctggaactg tactcttcac tgctttatca gcaaatgagc
      121 gcctggtgca gctatcatac cttcgaaggt gctgccgcgt tcctgcgccg tcacgcccag
      181 gaagagatga cgcatatgca gcgtctgttt gattacctga ctgataccgg caacttaccg
      241 cgtattaata ccgttgaatc tccgtttgct gaatattcct cacttgatga attattccag
      301 gaaacctata aacacgaaca attaatcacg cagaaaatta acgaactggc tcatgctgca
      361 atgaccaatc aggactaccc aacatttaat ttcctgcaat ggtatgtttc tgagcagcat
      421 gaagaagaga aactgttcaa atcgattatt gataaattaa gccttgcagg caaaagcggc
      481 gaaggtctgt attttatcga caaagaactc tctaccctcg acacacaaaa ctaatgctct
      541 ccggcggcag gttttctgcc gccgtttcaa atcagtgacg gcaaatctta ctctgatcgg
      601 ttgaaaaacc ttcttctgaa ggtataaatt tgccttttgc tgccagaaac gccaccaact
      661 ctcccgcagt catcccttct gccgagcagg tgtgaaaacg tgcctgttca ccaaaacgcg
      721 ctttaattgc tgcttccaga ctggcatgcg tatattgctc gcctgattca atcatcatat
      781 ttaacacttc atgaccgtga atagagtcca tcgttcctcc tcaaaaaaag cctagcgtag
      841 cgattgccgc ttatgaagac tttgcgccag cgcagaactg aacgcttttt gttgtacatt
      901 tatatttaca ccatatgtaa cgtcggtttg acgaatcagc cgttatgcct taacctgcgc
      961 cgcagatatc actcataaag atcgtcagga cagaagaaag cgtgaaaaac agaactctgg
     1021 gaagtgtttt tatcgtggcg ggaaccacaa ttggcgcagg catgctggca atgccgctgg
     1081 ctgcggcagg tgttggtttt agcgtcacgt taatcttgtt gattgggctt tgggcgttga
     1141 tgtgctacac ggcgctatta ctgctggagg tgtaccagca tgtcccggca gataccggtc
     1201 tgggcacgct ggcaaaacgc tatctgggac gctacggtca gtggctgacg ggcttcagta
     1261 tgatgttttt aatgtatgct ctgactgcgg catacatcag cggtgccggt gaattgttgg
     1321 cctccagcat cagcgactgg acaggtattt caatgtcggc aaccgctggc gtgctgttgt
     1381 tcacttttgt tgccggtggc gtggtttgtg tcggaacttc gctggtcgat ttatttaacc
     1441 gttttctgtt cagcgccaaa attatttttc tggtggtgat gctggtattg ctgctaccgc
     1501 atattcacaa agtgaatctt ttaaccctgc cgttgcaaca gggactggca ctgtctgcaa
     1561 tcccggtgat ttttacgtcg tttggttttc acggtagcgt gccgagtatt gtcagctata
     1621 tggatggcaa cattcgtaag ctacgctggg tgtttataat cggtagtgcg atccccctgg
     1681 tggcatatat tttctggcag ctggcgaccc ttggcagcat tgattcaaca acctttatgg
     1741 gattgctggc taatcatgcc ggattaaacg ggctgttaca ggcgttacgc gaaatggtgg
     1801 cctctccgca tgttgagctg gcagtgcatt tatttgctga tttagccctc gccacgtcct
     1861 tcctcggcgt agcgttaggc ttatttgatt atctggcaga tttgtttcag cgttcaaata
     1921 ccgttggtgg acgattgcaa actggtgcaa ttacctttct gccgccactg gcgtttgcac
     1981 tgttttatcc acgaggattt gtgatggcgc tgggttacgc cggtgtggca ctggcggtac
     2041 tggcattgat tatcccttcg ctgttgacct ggcaaagcag gaagcacaat cctcaggcgg
     2101 gttaccgggt gaaaggtggt cgtccggcgc tggtggtggt gtttctctgt ggtattgctg
     2161 tgattggcgt gcaatttttg attgcggcag gcttgttacc agaagtgggg tgattagata
     2221 gcctcaaaat tccttattgg gtgccagaat taacgctgac acccaatgtg acctcttaat
     2281 gcaggcagca ctgcttaaat ttcttaccac taccgcacgg acaaggatcg ttacgccccg
     2341 gtttctcttc tgctttgatc ggttgctgaa cagctttttc ctgcggatgc gccatccagt
     2401 atgcatgtaa atcaagcgcc gccagccgaa tagcatctac gctctcttca aacgcttctg
     2461 gcgacatctt ctctacccgc tcgaagtttt cctcagtacc gtgcagcgca atcgcatcca
     2521 gcgctggttt taacgaatcg ggcaacgttg accagtcaga aagtgccacg ccccgcatat
     2581 agccaaagca ccactcctca acaatcgtca gctcgctgcc atcgacttct cgcaagccga
     2641 ataacggctc aaattgctcc gggaattcgt tcagacgctc tgcggtatcg gccatatgtt
     2701 gaaaagccag gttcataaag cgcgtcatct ctttctctga cgcccagcgc ggcacatagt
     2761 cagccccacc ccacacggca accagccact gtgccggttc aatctcttgc ggagaactca
     2821 acaccgccgt caataaaccg tccagctccg ccacatcaag aatggcgtgg tcagtgttgt
     2881 atttggtcag aatatcgtcc agccattcca actcactttc gtttaacggt cccgttttca
     2941 tacgcttttc cttgtggatc tcaactcgcc agcacctatc ttacatgccg gtccgtatca
     3001 gagatacttt ttgagtggct ttgccggtga ttaaaaatta aggagggtgt aacgacaagt
     3061 tgcaggcaca aaaaaaccac ccgaaggtgg tttcacgaca ctgcttattg ctttgatttt
     3121 attcttatct ttcccatggt acccggagcg ggacttgaac ccgcacagcg cgaacgccga
     3181 gggattttaa atcccttgtg tctaccgatt ccaccatccg ggctcgggaa gaaagtggag
     3241 gcgcgttccg gagtcgaacc ggactagacg gatttgcaat ccgctacata accgctttgt
     3301 taacgcgcca aattcttcag gcctttcagc cagacatccg cttgacgccg atgtctttta
     3361 aactggagcg ggaaacgaga ctcgaactcg cgaccccgac cttggcaagg tcgtgctcta
     3421 ccaactgagc tattcccgca ttcatcaagc aatcagttaa tcacttgatt ttattatcgt
     3481 ctggcaatca gtgccgccgt tcgatgcgtt gcattctact tacctggcgc gatgagtcaa
     3541 cgatattttt caccactttt gatcgtttgc tgaaaattgc gccgaaacga tcactgatca
     3601 agcaaatctg cacgcgcagc gctcaaatat tgcaacattg accacagagt cagtaccgca
     3661 gccacaaaga aaagtgcaat accggcgtac tcaacccaaa tattcggacg ccacagcagc
     3721 catgccaacg ccaccatctg ggcagtggtt ttcacttttc caatccagga gacagccacg
     3781 ctactgcgtt tacccaactc cgccatccat tcgcgtagcg cagaaataat aatttcacgg
     3841 gcgatcatcg ttgctgccgg taaggtcacc caccagctgt ggtaatgctc ggttaccagc
     3901 accatggcga tagccacgag aactttatct gcgacagggt caaggaaagc accaaaccgg
     3961 gtactctggt tccagcgacg tgccagaaaa ccatcgaacc agtcagtcac cgccgcgacg
     4021 cagaaaatga gcgcggcggc aaacggcgac caggtgacag gcagataaaa gaccaataca
     4081 aagaatggga taaggatgac acggaacagt gtaagcaacg tagggatatt aaattgcata
     4141 atgacgggta actatctgtt gtcagtaaga ttacccctat gttgctacag agacatcaat
     4201 gtttcaacga ccagaagatc ttttctgcca gaccttgcga aatacccggc acttttgcaa
     4261 tttcctcgac gctggcgtta cgtaaacctt gcaaaccacc catatatttc aacaacattt
     4321 gccgacgttt tggcccgacg ccttcaatgg tttccaggga actggtattt ttgaccttcg
     4381 cccgtttttt acggtgccca ccaatcgcgt gatcgtgtga ttcatcgcga atatgctgga
     4441 taacatgcag cgcgggagaa tctggcggca aactaaatcc ctcaccttcc ggctcaaaga
     4501 acagcgtttc cagcccagcc ttacgatctg ctcctttggc aacgccaagt agcagcggat
     4561 gatttttatc ccatgagaca tccagttcgg cgaagacatt tttcgcctgc gcaagctggc
     4621 ctttgccgcc gtcgataaga atcacatccg ggatcttact gtcgtcaatg gctttaccat
     4681 aacgccgacg cagcacctga ttcatcgccg cataatcatc gcccggcgtg atgccagtaa
     4741 tgttatagcg ccgatactcc gcacgcagcg ggccgttagc atcaaacacc acacaggaag
     4801 cgacggtttg ttcacccatg gtatggctga tgtcaaagca ctccatccgc ttcacttccg
     4861 gcaatttcaa cacgctggca agcgcggtca gccgctggtg aacggtagat tgctgcgaaa
     4921 gtttgctggt taaggccgtc gccgcattgg tgcgcgcgag tttcagataa cgcgctctat
     4981 cgccacgagg tttggtttga acattaatct tgcgtcccgc cagttctgaa agggaatcgg
     5041 cgagcagcgt tttatcgcta agattaaaat cgagcaggat ctcacccggt aaggtgcgca
     5101 tctggctgcc ttgtaaatag aactgaccta cgaaggtttc caccacctcg ctcagttccg
     5161 tacctccagg cactttcggg aaatagctgc ggctgccgag cactttgccc tgacgaatga
     5221 acaatacgtg gacacaagcc atacccgcat cgaacgccac tccaataacg tcaaggtcgt
     5281 cgccggtatt ggaaacaaac tgtttttcgg tgacacgtcg caccgcctga atttggtcgc
     5341 gaatccgcgc cgcttcttca aactccagat tctggcttgc agtttccata cgactaatga
     5401 gttgcgtaag cacctgatca tctttgccag acaaaaacaa gcgcacatac tcgacctgct
     5461 gagcgtattc ttcttcactc accagtcctt caacgcacgg ccccagacag cgccctatct
     5521 gatattgcag acacggacgc gagcgattgc gataaacact gttttcgcac tggcgaatgg
     5581 gaaaaatctt ttgcagtagc gccagtgttt cacgtacggc atagccattc gggaacgggc
     5641 cgaaatattc acctttggca tgcttcgctc cacgatgcat cgccagacgc gggtgggtat
     5701 cgccactcag gaagataaaa ggatatgatt tatcatcgcg tagcaaaacg ttgtaacgcg
     5761 gctgataaag tttgatgtag ttgtgttcca gtaacagcgc ttcggtttct gtatgagtaa
     5821 ccgttacatc aatttgctgg atctgggcga ccagcgcttc ggttttgcgc gaagcgaggt
     5881 tgctacggaa atagctggaa agccgttttt tcaggtcttt cgctttgccg acatagataa
     5941 ccgtaccacc agcatcgtac atgcgataaa cgcctggctg gctggttacg gtttttaaaa
     6001 acgcttttgc gtcaaactga tcactcactg acttgataat gtctccgcat tacacagacc
     6061 atggcgaatt gccaggtgag tcagctcaac atcgccatga atgtttagtt tactgaacat
     6121 acgatagcgg tagctgttca ccgttttcgg actgagattg agctgttctg agatctcatt
     6181 gaccttctgg cccttggtga tcatcagcat aatctgcaat tcacgttcag acaaactggc
     6241 aaatgggctt tctgtttttt ctggttcgat ctggcttaac gccatttgtt gagcgatatc
     6301 agaagcaatg taacgctgcc ctgaatagac agaacgaatc gcactcacga cttcctgtgg
     6361 agccgcgcct ttgctgaggt agcccgcagc tccggcctgc atgactttcg ctggtaaagg
     6421 gttttctgta tggacggtaa gcatgatgat tttgacatca gctgtggaac gcgcgatttt
     6481 acgcgtcgcc tcaagaccgc caatgcccgg catactcatg tccattagca ccacgtcaac
     6541 ggcatttgcc cggcaccact taacggcgtc ttcaccgcac gatgcctcac cgacgacttt
     6601 tatacccttt atatcttcca gaatgcgtcg tatccctgcg cgcaccagtt cgtggtcatc
     6661 aacaagtaga acgttgatca aaggaatatc tccagaaata gggataacgc tactgatagt
     6721 tagtcattcg tatattagcg gtttttattg caactttgaa acgttaaaaa tggtgggtat
     6781 tcgatttttc tctcgttttt gtcaattcga ttgtccacag ttatggtcac gcccgtcacc
     6841 aacggcttcc gggatcgttt cacagtaagc ccggcgatga aatgtgacaa aaattgactt
     6901 tattcagcaa aaatgaaaat cagcccgctg aatatgtaac attaattaac ccgaacaata
     6961 cgggcaaaaa acattcatga taaaaatatt tattggtca
//
LOCUS       DADSYM010000188         6998 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2415
            SAMN08039579-rid11723173.denovo.190, whole genome shotgun sequence.
ACCESSION   DADSYM010000188 DADSYM010000000
VERSION     DADSYM010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039579
            Sequence Read Archive: SRR6362533
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6998)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6998)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980162.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 03:39:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,484
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 4,347
            CDSs (with protein)               :: 4,347
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 43 of 85
            Pseudo Genes (incomplete)         :: 37 of 85
            Pseudo Genes (internal stop)      :: 19 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6998
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039579-rid11723173.denovo.190"
                     /strain="LGE2415"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8302 N 95.9628 W"
                     /collection_date="2015-08-24"
     gene            219..1277
                     /gene="citC"
                     /locus_tag="J1Y83_003973"
     CDS             219..1277
                     /gene="citC"
                     /locus_tag="J1Y83_003973"
                     /EC_number="6.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415151.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[citrate (pro-3S)-lyase] ligase"
                     /protein_id="HBA7632071.1"
                     /translation="MFGNDIFTRVKRSENKKMAEIAQFLHENDLSVDTTVEVFITVTR
                     DEKLIACGGIAGNIIKCVAISESVRGEGLALTLATELINLAYERHSTHLFIYTKTEYE
                     ALFRQCGFSTLTSVPGVMVLMENSATRLKRYAESLKKFRHPGNKIGCIVMNANPFTNG
                     HRYLIQQAAAQCDWLHLFLVKEDSSRFPYEDRLDLVLKGTADIPRLTVHRGSEYIISR
                     ATFPCYFIKEQSVINHCYTEIDLKIFRQYLAPALGVTHRFVGTEPFCRVTAQYNQDMR
                     YWLETPTISAPPIELVEIERLRYQEMPISASRVRQLLAKNDLTAIAPLVPAVTLHYLQ
                     NLLEHSRQDAAARQKTPA"
     gene            1292..1588
                     /gene="citD"
                     /locus_tag="J1Y83_003974"
     CDS             1292..1588
                     /gene="citD"
                     /locus_tag="J1Y83_003974"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase acyl carrier protein"
                     /protein_id="HBA7632072.1"
                     /translation="MKINQPAVAGTLESGDVMIRIAPLDTQDIDLQINSSVEKQFGDA
                     IRTTILDVLARYNVRGVQLNVDDKGALDCILRARLEALLARASGIPALPWEDCQ"
     gene            1585..2493
                     /gene="citE"
                     /locus_tag="J1Y83_003975"
     CDS             1585..2493
                     /gene="citE"
                     /locus_tag="J1Y83_003975"
                     /EC_number="4.1.3.34"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415149.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate (pro-3S)-lyase subunit beta"
                     /protein_id="HBA7632073.1"
                     /translation="MISASLQQRKTRTRRSMLFVPGANAAMVSNSFIYPADALMFDLE
                     DSVALREKDTARRMVYHALQHPLYRDIETIVRVNALDSEWGVNDLEAVVRGGADVVRL
                     PKTDTAQDVLDIEKEILRIEKACGREPGSTGLLAAIESPLGITRAVEIAHASERLIGI
                     ALGAEDYVRNLRTERSPEGTELLFARCSILQAARSAGIQAFDTVYSDANNEAGFLQEA
                     AHIKQLGFDGKSLINPRQIDLLHNLYAPTQKEVDHARRVVEAAEAAAREGLGVVSLNG
                     KMVDGPVIDRARLVLSRAELSGIREE"
     gene            2504..4036
                     /gene="citF"
                     /locus_tag="J1Y83_003976"
     CDS             2504..4036
                     /gene="citF"
                     /locus_tag="J1Y83_003976"
                     /EC_number="2.8.3.10"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase subunit alpha"
                     /protein_id="HBA7632074.1"
                     /translation="MTQKIEQSQRQERVAAWNRRAECDLAAFQNSPKQTYQAEKARDR
                     KLCANLEEAIRRSGLQDGMTVSFHHAFRGGDLTVNMVMDVIAKMGFKNLTLASSSLSD
                     CHAPLVEHIRQGVVTRIYTSGLRGPLAEEISRGLLAEPVQIHSHGGRVHLVQSGELNI
                     DVAFLGVPSCDEFGNANGYSGKACCGSLGYAMVDADNAKQVVMLTEELLPYPHNPASI
                     EQDQVDLIVKVDRVGDAAKIGAGATRMTTNPRELLIARSAADVIVNSGYFKEGFSMQT
                     GTGGASLAVTRFLEDKMRSRDIRADFALGGITATMVDLHEKGLIRKLLDVQSFDSHAA
                     QSLARNPNHIEISANQYANWGSKGAAVDRLDVVVLSALEIDTQFNVNVLTGSDGVLRG
                     ASGGHCDTAIASALSIIVAPLVRGRIPTLVDNVLTCITPGSSVDILVTDHGIAVNPAR
                     PELAERLQEAGIKVVSIEWLRERARLLTGEPQPIEFTDRVVAVVRYRDGSVIDVVHQV
                     KE"
     gene            4040..4591
                     /gene="citX"
                     /locus_tag="J1Y83_003977"
     CDS             4040..4591
                     /gene="citX"
                     /locus_tag="J1Y83_003977"
                     /EC_number="2.7.7.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase holo-[acyl-carrier protein]
                     synthase"
                     /protein_id="HBA7632075.1"
                     /translation="MHLLPELASHHAVSIPELLVSRDERQARQHAWLKRHPVPLVSFT
                     VVAPGPIKDSEVTRRIFNHGVTALRALAAKQGWQIQEQAALVSASGPEGMLSIAAPAR
                     DLKLATIELEHSHPLGRLWDIDVLTPEGDILSRRDYSLPPRRCLLCEQSAAVCARGKT
                     HQLTDLLNRMEALLNDVDACNVN"
     gene            4566..5444
                     /gene="citG"
                     /locus_tag="J1Y83_003978"
     CDS             4566..5444
                     /gene="citG"
                     /locus_tag="J1Y83_003978"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase CitG"
                     /protein_id="HBA7632076.1"
                     /translation="MSMPATSTKTTKLATSLIDEYALLGWRAMLTEVNLSPKPGLVDR
                     INCGAHKDMALEDFHRSALAIQGWLPRFIEFGACSAEMAPEAVLNGLRPIGMACEGDM
                     FRATAGVNTHKGSIFSLGLLCAAIGRLLQLNQPVTPTTVCSTAASFCRGLTDRELRTN
                     NSRLTAGQRLYQQLGLTGARGEAEAGYPLVINHALPHYLTLLDQGLDPELALLDTLLL
                     LMATNGDTNVASRGGEGGLRWLQREAQTLLQKGGIRTPADLDYLRQFDRECIERNLSP
                     GGSADLLILTWFLAQI"
     gene            5495..6958
                     /gene="citT"
                     /locus_tag="J1Y83_003979"
     CDS             5495..6958
                     /gene="citT"
                     /locus_tag="J1Y83_003979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate/succinate antiporter CitT"
                     /protein_id="HBA7632077.1"
                     /translation="MSLAKDNIWKLLAPLVVMGVMFLIPVPDGMPPQAWHYFAVFVAM
                     IVGMILEPIPATAISFIAVTICVIGSNYLLFDAKELADPAFNAQKQALKWGLAGFSST
                     TVWLVFGAFIFALGYEVSGLGRRIALFLVKFMGKRTLTLGYAIVIIDILLAPFTPSNT
                     ARTGGTVFPVIKNLPPLFKSFPNDPSARRIGGYLMWMMVISTSLSSSMFVTGAAPNVL
                     GLEFVSKIAGIQISWLQWFLCFLPVGVILLIIAPWLSYVLYKPEITHSEEVATWAGDE
                     LKTMGALTRREWTLIGLVLLSLGLWVFGSEVINATAVGLLAVSLMLALHVVPWKDITR
                     YNSAWNTLVNLATLVVMANGLTRSGFIDWFANTMSTHLEGFSPNATVIVLVLVFYFAH
                     YLFASLSAHTATMLPVILAVGKGIPGVPMEQLCILLVLSIGIMGCLTPYATGPGVIIY
                     GCGYVKSKDYWRLGAIFGVIYISMLLLVGWPILAMWN"
BASE COUNT         1576 a         1901 c         1859 g         1662 t
ORIGIN      
        1 ttaaaaccat taaataaata aaaaaccact aactctatgt gaaataaatc aaaatttcac
       61 gccgaaatac tccttaggat gtatagcgaa aggagaaaaa gatatacctc gatcgccccc
      121 ctttctccca agtgaaaata aaaggttatc aatttgcaac attgaacaac attcgttgca
      181 aatcgataac aacatgcacc ttcaggatac tatttattat gttcggcaat gatattttca
      241 cccgcgtaaa acgttcagaa aataaaaaaa tggcggaaat cgcccaattc ctgcatgaaa
      301 atgatttgag cgttgacacc acagtcgaag tatttattac cgtaacccgc gatgaaaagc
      361 ttatcgcgtg cggtggaatt gccggaaata ttattaaatg cgttgctatc agtgaatccg
      421 ttcgcggtga aggactggcg ctgacattag ccactgaact gataaacctc gcctatgagc
      481 ggcatagcac gcatctgttt atttatacca aaaccgaata cgaggcgctg ttccgccagt
      541 gcggtttttc cacgctgacc agcgtacccg gcgtgatggt gctgatggaa aacagcgcca
      601 cgcgactgaa acgctatgcc gaatcgctga aaaaatttcg tcatccaggg aacaagattg
      661 gctgcattgt gatgaacgcc aatcccttta cgaatggtca ccgttatctg attcaacagg
      721 ctgcggcaca gtgcgactgg ttgcatctgt ttttagtcaa agaagattct tcacgcttcc
      781 cctatgaaga ccggctggat ctggtgttaa aaggcaccgc cgatattcca cgcctgaccg
      841 tgcatcgcgg ctccgaatac atcatctccc gcgctacgtt cccttgctac ttcattaaag
      901 aacagagcgt cattaaccat tgttacaccg aaattgatct gaagattttc cgtcagtacc
      961 tcgctcccgc gctgggtgta actcaccgct ttgtcggtac tgaacccttt tgtcgcgtta
     1021 ccgcccagta caaccaggat atgcgctact ggctggaaac gccgactatc tccgcaccgc
     1081 ccatcgaact ggttgaaatt gagcggctgc gttaccagga gatgccgata tccgcttccc
     1141 gggtacgtca actgctggcg aaaaacgatc tcacggctat cgcgccgctg gtccctgcag
     1201 tcacgctgca ttatttgcag aacctgcttg agcactcccg ccaggacgcg gcagctcgtc
     1261 aaaagacccc cgcatgagaa acaggtgaaa aatgaaaata aaccagcccg ccgttgcagg
     1321 cacccttgag tctggggatg tgatgatacg catcgcccca ctcgatacgc aggatatcga
     1381 cctgcaaatc aacagcagcg ttgagaaaca gtttggcgat gcaattcgca ccaccattct
     1441 ggacgttctc gcccgctaca acgtgcgcgg cgtacagctg aatgtcgatg acaaaggcgc
     1501 actggactgc attttacgtg cacgactgga agccctgctg gcacgcgcca gcggtatccc
     1561 ggctctgcca tgggaggatt gccaatgatt tccgcttcgc tgcaacaacg taaaactcgc
     1621 acccgccgca gtatgttgtt tgtgccaggt gccaatgccg cgatggtcag caactccttc
     1681 atctacccgg ctgatgccct gatgtttgac ctcgaagact ccgtggcatt gcgtgaaaaa
     1741 gacaccgccc gccgcatggt ttaccacgcg ctgcaacatc cgctgtatcg cgatattgaa
     1801 accattgtgc gtgtaaacgc gctggattcc gaatggggtg ttaacgacct ggaagccgtc
     1861 gttcgcggtg gtgcggacgt tgtgcgtctg ccgaaaaccg ataccgctca ggatgttctg
     1921 gatatcgaaa aagagatcct gcgcatcgaa aaagcctgtg gtcgtgaacc cggcagcacc
     1981 ggcctgctgg cagcgattga atctccgctg ggcattaccc gcgcagtgga aatcgctcac
     2041 gcttccgagc gtttgatcgg tatcgccctc ggtgcagaag actatgtgcg caacctgcgt
     2101 acagaacgct ccccggaagg aactgaactg ctgttcgctc gctgctccat tttgcaggcc
     2161 gcgcgctctg cgggtattca ggcgttcgat accgtctatt ccgacgctaa caacgaagcc
     2221 ggattcctgc aagaagccgc ccacatcaaa cagctgggct ttgacggcaa atcgctgatc
     2281 aacccgcgtc agattgatct gctgcacaac ctctacgcac cgacccagaa agaagtggat
     2341 cacgcccgcc gcgtcgtaga agccgctgaa gccgccgctc gcgaaggcct cggcgtggtt
     2401 tccctgaacg gcaagatggt ggacggtccg gttatcgatc gcgcccgtct ggtgctctcc
     2461 cgtgcagaac tttccggcat ccgcgaagaa taaggcaatc aaaatgacgc agaaaattga
     2521 acaatctcaa cgacaagaac gggtagcggc ctggaatcgt cgcgctgaat gcgatcttgc
     2581 cgctttccag aactcgccaa agcaaaccta ccaggctgaa aaagcgcgcg atcgcaaact
     2641 gtgcgccaac ctggaagaag cgattcgtcg ctctggttta caggacggca tgacggtttc
     2701 cttccatcac gctttccgtg gcggtgacct gaccgtcaat atggtgatgg acgtcatcgc
     2761 gaagatgggc tttaaaaacc tgactctggc ctccagctcc ctgagtgatt gccatgcgcc
     2821 actggtagaa cacattcgcc agggcgtggt tacccgcatt tatacctctg gcctgcgcgg
     2881 tccactggcg gaagaaatct cccgtggtct gctggcggaa ccggtgcaaa tccactctca
     2941 cggcggacgt gtgcatctgg tacagagcgg cgaactgaat atcgatgtgg ctttcctcgg
     3001 cgtcccgtcc tgtgatgaat tcggtaatgc caacggctac tccggtaaag cctgctgcgg
     3061 ctccctcggc tatgcaatgg ttgatgccga caacgcaaaa caggtcgtga tgcttaccga
     3121 agaactgctg ccttatccgc ataatccggc aagcattgag caagatcagg ttgatctgat
     3181 cgtcaaagtt gatcgcgttg gcgatgctgc aaaaatcggc gctggtgcta cccgtatgac
     3241 cactaacccg cgcgaactgc ttattgcccg tagcgctgcg gatgtgattg ttaactctgg
     3301 ctacttcaaa gaaggtttct ccatgcagac cggcaccggc ggcgcttcgc tggcggtaac
     3361 ccgtttcctg gaagacaaaa tgcgtagccg cgatattcgc gccgacttcg ccctcggcgg
     3421 tattaccgca acgatggttg atctgcacga aaaaggtttg atccgcaaac tgctggatgt
     3481 gcagagcttt gacagccatg ctgcgcaatc gctggcccgt aaccccaatc acatcgaaat
     3541 cagcgccaac cagtacgcta actgggggtc gaaaggcgca gcggttgatc gtctcgacgt
     3601 ggtggtactg agcgcgctgg aaattgacac ccagttcaac gttaacgtgc tgaccggctc
     3661 tgacggcgta ctgcgtggtg cttccggtgg tcactgcgat accgcgattg cttctgcgct
     3721 ttccatcatc gtcgcgccgc tggtacgcgg tcgtattccg acgctggtgg ataacgtgct
     3781 gacctgcatc accccgggct ccagtgtcga tattctggtc acagaccacg gcatcgctgt
     3841 taacccggca cgtccggagc tggcagaacg tctgcaggaa gcggggatca aagtggtttc
     3901 tattgagtgg ctgcgtgaac gtgcgcgtct gctgaccggt gaaccacagc cgattgaatt
     3961 caccgaccgc gtcgttgccg ttgtgcgtta ccgcgatggc tcggtgatcg atgttgtgca
     4021 tcaggtgaag gaataagcca tgcacctgct tcctgaactc gccagccacc atgcggtgtc
     4081 aattcccgag ctgctcgtca gccgggatga aaggcaagca cggcaacacg cctggctcaa
     4141 gcgccatcct gttccactgg tctcctttac cgtggttgcg cctgggccga ttaaagacag
     4201 cgaggtcaca cgccgaattt ttaatcatgg cgtgacagcc ttgcgtgcct tagccgcaaa
     4261 acagggctgg caaattcagg agcaggctgc actggtttct gccagcgggc cggagggcat
     4321 gttgagcatt gccgccccgg ctcgcgacct caagctcgcc accattgagc ttgaacatag
     4381 tcatcctctc gggcggttat gggatatcga tgtcctgacg cccgaaggcg acattctctc
     4441 ccgccgcgat tattcactgc cgcctcgccg ctgcctgttg tgcgagcaaa gcgcagctgt
     4501 ttgcgcgcgt ggaaaaaccc atcaactgac cgatttactc aaccgcatgg aggcgctgct
     4561 aaacgatgtc gatgcctgca acgtcaacta aaaccacaaa gcttgcgacg tcattaatcg
     4621 atgagtacgc cctgctgggc tggcgcgcca tgctgactga agtcaacctg tcaccgaaac
     4681 caggcctcgt ggatcgcatt aactgcggtg cgcacaaaga tatggcgctg gaagatttcc
     4741 accgcagcgc gctggcgatt cagggctggt taccccgttt cattgaattt ggtgcctgta
     4801 gtgcagaaat ggcaccggaa gcggtactca acggattacg cccaattggt atggcttgcg
     4861 aaggtgatat gttccgcgcc actgcgggcg taaacacgca taaaggaagc attttttctt
     4921 tagggctact gtgtgcggca attggccgtt tgcttcagct caaccagccg gtaacgccaa
     4981 caaccgtttg ttctacggcg gcaagtttct gccgtggcct gaccgatcgc gaactgcgta
     5041 ccaataattc acgactgacg gcaggtcaac ggttgtacca gcagcttggc cttaccggcg
     5101 cacgcggtga agccgaagcg ggttatccac tggtaatcaa ccacgccttg ccgcattacc
     5161 tcactctgct ggatcagggg ttagatcctg agctggcatt gctcgatacc ttgctcctac
     5221 tgatggctac caacggcgat accaacgttg catcgcgcgg tggcgagggg ggcctgcgct
     5281 ggctacagcg cgaggcgcaa acattattgc aaaaaggggg cattcgaacc cccgccgatc
     5341 tcgattatct ccggcagttc gacagggagt gtatcgaacg aaatctcagt ccaggcggca
     5401 gtgccgacct gctgatcctt acctggtttt tagcacagat ttaattattt aagcacttga
     5461 taaatttgga aatattaatt ttcggagaac ccgtatgtct ttagcaaaag ataatatatg
     5521 gaaattattg gcgcccctgg tggtgatggg tgtcatgttt cttatccctg tacctgacgg
     5581 tatgccgccg caggcgtggc attactttgc agtgtttgtg gcaatgattg tcggcatgat
     5641 cctcgagcca attccggcaa cggcgatcag ttttattgcg gttactattt gcgttattgg
     5701 cagtaattac ctgctctttg atgccaaaga attagctgac ccagcgttta atgcgcaaaa
     5761 acaggcgctg aaatggggtc tggctggttt ttccagcacc acggtatggc tggtatttgg
     5821 cgcatttatt tttgcattag ggtatgaagt ttccgggtta ggccgtcgta ttgccctttt
     5881 cctggtgaaa ttcatgggca aacgcacgct gacgttgggt tatgcgattg tcattatcga
     5941 cattctgctg gcaccgttta caccgtccaa caccgcgcgt accgggggta cggtttttcc
     6001 ggtcattaaa aacctgccgc cgctgtttaa atcattcccg aacgatccgt ccgcgcgtcg
     6061 tattggcggc tatttgatgt ggatgatggt cattagtacc agtctgagtt cgtccatgtt
     6121 tgtcaccggt gcggcaccaa acgtgctggg tctggagttc gtcagcaaaa ttgccggtat
     6181 ccagattagc tggttgcagt ggttcctctg cttcctgccg gttggggtta tcttgcttat
     6241 cattgcgccg tggctttcct acgtgctgta caaaccggaa atcacacaca gtgaagaagt
     6301 ggcaacctgg gcgggtgatg aactaaaaac catgggtgcg ctgacgcgca gagagtggac
     6361 gctgattggc cttgtattgc tcagcttagg tttgtgggta tttggcagtg aagtcattaa
     6421 tgctactgcg gtaggtctgc tggcagtttc gctgatgctg gcgctgcacg ttgtgccgtg
     6481 gaaagacatt actcgctata acagcgcatg gaacacgctg gtcaacctgg caacgctggt
     6541 tgttatggcg aatggcctga cccgttctgg ctttatcgac tggttcgcca acacgatgag
     6601 tacgcacctg gaaggcttct caccaaacgc aacggtgatt gtactggttc tggtgttcta
     6661 ctttgcccac tacctgtttg ccagcctgtc tgcgcacacc gcaaccatgc tgccggttat
     6721 tctggccgtc ggtaaaggga ttccgggcgt accaatggaa caactgtgta tcctgctggt
     6781 gctgtctatc ggtatcatgg gctgtctgac gccgtatgca accggtcctg gggtgattat
     6841 ttacggctgt ggctatgtga aatcaaaaga ttactggcgt cttggcgcaa tcttcggggt
     6901 gatttacatc tctatgctgc tgttggttgg ctggccgatt ctcgccatgt ggaactaata
     6961 cgttcgccaa acgctgaata aatcatcacg cccgccag
//
LOCUS       DADSYM010000189         6958 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2415
            SAMN08039579-rid11723173.denovo.191, whole genome shotgun sequence.
ACCESSION   DADSYM010000189 DADSYM010000000
VERSION     DADSYM010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039579
            Sequence Read Archive: SRR6362533
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6958)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6958)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980162.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 03:39:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,484
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 4,347
            CDSs (with protein)               :: 4,347
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 43 of 85
            Pseudo Genes (incomplete)         :: 37 of 85
            Pseudo Genes (internal stop)      :: 19 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6958
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039579-rid11723173.denovo.191"
                     /strain="LGE2415"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8302 N 95.9628 W"
                     /collection_date="2015-08-24"
     gene            485..1474
                     /locus_tag="J1Y83_003980"
     CDS             485..1474
                     /locus_tag="J1Y83_003980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001397210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter substrate-binding
                     protein"
                     /protein_id="HBA7632078.1"
                     /translation="MHKFTKALAAIGLAAVMSQSAMAENLKLGFLVKQPEEPWFQTEW
                     KFADKAGKDLGFEVIKIAVPDGEKTLNAIDSLAASGAKGFVICTPDPKLGSAIVAKAR
                     GYDMKVIAVDDQFVNAKGKPMDTVPLVMMAATKIGERQGQELYKEMQKRGWDVKESAV
                     MAITSNELDTARRRTTGSMDALKAAGFPGKQIYQVPTKSNDIPGAFDAANSMLVQHPE
                     VKHWLIVGMNDSTVLGGVRATEGQGFKASDIIGIGINGVDAVSELSKAQATGFYGSLL
                     PSPDVHGYKSSEMLYNWVAKGVEPTKFTEVTDVVLITRDNFKEELEKKGLGGK"
     gene            1544..3058
                     /locus_tag="J1Y83_003981"
     CDS             1544..3058
                     /locus_tag="J1Y83_003981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose ABC transporter ATP-binding protein
                     AraG"
                     /protein_id="HBA7632079.1"
                     /translation="MQQSTPYLSFRGIGKTFPGVKALTDISFDCYAGQVHALMGENGA
                     GKSTLLKILSGNYAPTTGSVVINGQEMSFSDTTAALNAGVAIIYQELHLVPEMTVAEN
                     IYLGQLPHKGGIVNRSLLNYEAGLQIKHLGMDIDPDTPLKYLSIGQWQMVEIAKALAR
                     NAKIIAFDEPTSSLSAREIDNLFRVIRELRKEGRVILYVSHRMEEIFALSDAITVFKD
                     GRYVKTFTDMQQVDHDALVQAMVGRDIGDIYGWQPRSYGEERLRLDAVKAPGVRTPIS
                     LAVRSGEIVGLFGLVGAGRSELMKGMFGGTQITAGQVYIDQQPIDIRKPSHAITAGMM
                     LCPEDRKAEGIIPVHSVRDNINISARRKHVLGGCVINNGWEENNADHHIRSLNIKTPG
                     AEQLIMNLSGGNQQKAILGRWLSEEMKVILLDEPTRGIDVGAKHEIYNVIYALAAQGV
                     AVLFASSDLPEVLGVADRIVVMREGEIAGELLHEQADERQALSLAMPKVSQAVA"
     gene            3070..4059
                     /locus_tag="J1Y83_003982"
     CDS             3070..4059
                     /locus_tag="J1Y83_003982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose ABC transporter permease AraH"
                     /protein_id="HBA7632080.1"
                     /translation="MMSSVSTSGSGAPKSSFSFGRIWDQYGMLVVFAVLFIACAIFVP
                     NFATFINMKGLGLAISMSGMVACGMLFCLASGDFDLSVASVIACAGVTTAVVINLTES
                     LWIGVAAGLLLGVLCGLVNGFVIAKLKINALITTLATMQIVRGLAYIISDGKAVGIED
                     ESFFALGYANWFGLPAPIWLTVACLIIFGLLLNKTTFGRNTLAIGGNEEAARLAGVPV
                     VRTKIIIFVLSGLVSAIAGIILASRMTSGQPMTSIGYELIVISACVLGGVSLKGGIGK
                     ISYVVAGILILGTVENAMNLLNISPFAQYVVRGLILLAAVIFDRYKQKAKRTV"
     gene            4226..5026
                     /gene="otsB"
                     /locus_tag="J1Y83_003983"
     CDS             4226..5026
                     /gene="otsB"
                     /locus_tag="J1Y83_003983"
                     /EC_number="3.1.3.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-phosphatase"
                     /protein_id="HBA7632081.1"
                     /translation="MTEPLTETPELSAKYAWFFDLDGTLAEIKPHPDQVVVPDTILQG
                     LQLLATASDGALALISGRSMVELDALAKPYRFPLAGVHGAERRDINGKTHIVYLPDAI
                     ARDISVQLHTVIAQYPGAELEAKGMAFALHYRQAPQHEDALMTLAQRITQIWPQMALQ
                     QGKCVVEIKPKGTSKGEAIAAFMQEAPFIGRTPVFLGDDLTDESGFAVVNRLGGMSVK
                     IGTGATQASWRLAGVPDVWSWLEMITTALQQKRENNRSDDYESFSRSI"
     gene            5001..6425
                     /gene="otsA"
                     /locus_tag="J1Y83_003984"
     CDS             5001..6425
                     /gene="otsA"
                     /locus_tag="J1Y83_003984"
                     /EC_number="2.4.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalose-phosphate synthase"
                     /protein_id="HBA7632082.1"
                     /translation="MSRLVVVSNRIAPPDEHAASAGGLAVGILGALKAAGGLWFGWSG
                     ETGNEDQPLKKVKKGNITWASFNLSEQDLDEYYNKFSNAVLWPAFHYRLDLVQFQRPA
                     WDGYLRVNALLADKLLPLLQDDDIIWIHDYHLLPFAHELRKRGVNNRIGFFLHIPFPT
                     PEIFNALPTYDTLLEQLCDYDLLGFQTENDRLAFLDCLSNLTRVTTRSAKSHTACGKA
                     FRTEVYPIGIEPKEIAKQAAGPLPPKLAQLKAELKNVQNIFSVERLDYSKGLPERFLA
                     YEALLEKYPQHHGKIRYTQIAPTSRGDVQAYQDIRHQLENEAGRINGKYGQLGWTPLY
                     YLNQHFDRKLLMKIFRYSDVGLVTPLRDGMNLVAKEYVAAQDPANPGVLVLSQFAGAA
                     NELTSALIVNPYDRDEVAAALDRALTMSLAERISRHAEMLDVIVKNDINHWQECFISD
                     LKQIVPRSAESQQRDKVATFPKLA"
     gene            complement(6432..6860)
                     /gene="uspC"
                     /locus_tag="J1Y83_003985"
     CDS             complement(6432..6860)
                     /gene="uspC"
                     /locus_tag="J1Y83_003985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspC"
                     /protein_id="HBA7632083.1"
                     /translation="MSYSNILVAVAVTPESQQLLAKAVSIARPVKGHISLITLASDPE
                     MYNQLAAPMLEDLRNVMQEETQSFLDKLIQDAGYPVDKTFIAYGELSEHILEVCRKHH
                     FDLVICGNHNHSFFSRASCSAKRVITSSEVDVLLVPLTGD"
BASE COUNT         1741 a         1626 c         1817 g         1774 t
ORIGIN      
        1 ttttcatttt tgcgatatgg ctctcattat acgtgttctg ttaattaatg agtacagtga
       61 taattaatgg cgaaatgaat gacgcgcatt ttccacatct ttaagttgcg gttattacac
      121 cattgcaaaa actcaacgcc aggtaatgag gcctattgac tggttaaaaa gaaaacatcc
      181 cgcatgggta ccaaagacaa caaggacttc caggctaatc ttatggatta ttctgctgtg
      241 cactagataa tttgtctgac aaattcgctt ttccttatgt cttttcccgc taaatttatg
      301 cacgttctca ctgtaattct gcgatgtgat attgctctcc tatggagaat taatttctcg
      361 ctaaaactat gtcaacacag tcacttatct tttagttaaa aggtaatgct ttgttttccg
      421 attaatttaa cgaatgtcat tcgtttttgc cctacacaaa acgacactaa agctggagag
      481 aaccatgcac aaatttacta aagccctggc agccattggt ctggcagccg ttatgtcaca
      541 atccgctatg gcggagaacc tgaagctcgg tttcctggtg aagcaaccgg aagagccgtg
      601 gttccagacc gaatggaagt ttgccgataa agccgggaaa gatttagggt ttgaagttat
      661 taagattgcc gtgccggatg gcgaaaaaac attgaacgct atcgacagcc tggccgccag
      721 tggcgcgaaa ggtttcgtta tttgtactcc ggacccgaaa ctcggctctg ccatcgtggc
      781 gaaagcgcgt ggctacgata tgaaagtcat tgccgtggat gaccagtttg ttaacgccaa
      841 aggtaagcca atggataccg ttccgctggt gatgatggct gcgaccaaaa ttggcgaacg
      901 tcagggccag gaactgtata aagagatgca gaaacgtggc tgggatgtca aagaaagtgc
      961 ggtgatggcg attacctcca acgaactgga tacggctcgt cgccgtacta cggggtctat
     1021 ggatgcgctg aaagctgcag gattcccggg aaaacaaatt tatcaggtac ctaccaaatc
     1081 taacgacatc ccgggggcat ttgacgctgc caactcaatg ctggttcaac atccggaagt
     1141 taaacattgg ctgattgtcg gtatgaacga cagcaccgtg ctgggcggcg tacgcgcgac
     1201 ggaaggtcag ggctttaaag cgagcgatat catcggtatc ggtattaacg gtgtggatgc
     1261 ggtgagtgaa ctgtctaagg cacaggcaac cggcttctac ggttccctgc tgccaagccc
     1321 ggacgtacat ggttataagt ccagcgaaat gctttacaac tgggttgcaa aaggtgttga
     1381 accgaccaaa ttcaccgaag ttaccgacgt ggtgctgatc acgcgtgaca actttaaaga
     1441 agaactggag aaaaaaggtt taggcggtaa gtaatttgcc ggaaaaattc ccctctgcat
     1501 gatgcagagg gggtgtgaac gaccagtgat tcacggagac gttatgcaac agtctacccc
     1561 gtatctctca tttcgcggca tcggtaaaac gtttcccggc gttaaggcgc tgacggatat
     1621 tagttttgac tgctatgccg gtcaggttca tgcgttgatg ggtgaaaatg gcgcaggaaa
     1681 atcaactctc ttaaaaatcc tcagcggaaa ctatgcgcca accacgggtt ctgtagtgat
     1741 taatgggcag gaaatgtcct tttccgacac gaccgcagca cttaacgcgg gggtggcgat
     1801 tatttaccag gaactgcatc tcgtgccgga aatgaccgtc gcggaaaaca tctatctcgg
     1861 ccagctgccg cataaaggcg gcattgtgaa tcgttcattg ctgaattatg aggcaggttt
     1921 acaaattaaa catcttggta tggatattga cccggacacg ccgctgaaat atctctccat
     1981 tggtcagtgg cagatggttg aaatcgccaa agcgctggcg cgtaacgcca aaattatcgc
     2041 ctttgatgag ccaaccagct ccctctctgc ccgcgaaatc gacaatcttt tccgcgttat
     2101 tcgtgaactg cgaaaagagg ggcgggtgat cttatacgtt tctcaccgta tggaagaaat
     2161 atttgccctc agcgatgcca tcaccgtctt taaagatggg cgttatgtca aaacctttac
     2221 cgatatgcag caggttgacc acgacgcact ggtgcaggcg atggtcgggc gagacattgg
     2281 cgatatctac ggctggcaac cgcgtagtta tggcgaggag cggctgcgtc ttgatgctgt
     2341 gaaagcacca ggcgtgcgta cgccaataag tctggcggtt cgcagtggag aaattgtcgg
     2401 tctgtttggt ctggtcgggg cagggcgtag cgaattaatg aaaggcatgt ttggcggtac
     2461 gcaaatcacc gccggtcagg tttatatcga ccaacagccg atcgatattc gtaaaccgag
     2521 ccacgctatt accgcaggca tgatgctctg cccggaagat cgcaaagcgg aaggcattat
     2581 tcctgtgcac tccgttcgcg acaatatcaa catcagtgcc agacgtaaac atgtgcttgg
     2641 aggttgtgta atcaacaacg gttgggaaga aaacaatgcc gatcaccaca ttcgttcgct
     2701 caacatcaaa acgccgggcg ctgagcaact gatcatgaat ctctcaggcg gaaatcagca
     2761 aaaagctatt ctgggccgct ggttatcgga ggagatgaag gtcattttgc tggatgaacc
     2821 tacgcgtggc attgatgttg gtgctaagca tgaaatttac aacgtgattt atgcgctggc
     2881 ggcgcagggt gtggcggtgc tgtttgcctc cagcgactta cctgaagtcc tcggcgttgc
     2941 cgaccggatt gtggtgatgc gcgaaggtga aatcgccggt gaattgttac acgagcaggc
     3001 agatgagcgt caggcactga gccttgcgat gcctaaagtc agccaggcag ttgcctgagt
     3061 aaggagagta tgatgtcttc tgtttctaca tcgggttctg gcgcacctaa gtcgtcattc
     3121 agcttcgggc gtatctggga tcagtacggc atgctggtgg tgtttgcggt gctttttatc
     3181 gcctgtgcca tttttgtccc aaattttgcc accttcatta atatgaaagg gttgggcctg
     3241 gcaatttcta tgtcggggat ggtggcgtgt ggcatgttgt tctgccttgc ttccggtgac
     3301 tttgaccttt ctgtcgcctc cgtaattgcc tgtgcgggtg tcaccactgc ggtggttatc
     3361 aacctgactg aaagcctgtg gattggcgtg gcagcgggtt tgttgctggg cgttctctgt
     3421 ggcctggtca atggctttgt tatcgccaaa ctgaaaataa atgccctcat cacgactctg
     3481 gcaacgatgc agattgttcg aggtctggcg tacatcattt cagacggtaa agcggtcggt
     3541 atcgaagatg aaagcttctt tgcccttggt tacgccaact ggtttggtct gcctgcgcca
     3601 atctggctca ccgtcgcgtg tttgatcatc tttggcttgc tgctgaataa aaccaccttt
     3661 ggtcgtaata ccctggcgat tggcgggaac gaagaggctg cgcgtctggc gggtgtaccg
     3721 gttgttcgca ccaaaattat tatctttgtt ctctctggcc tggtatcagc gatagccgga
     3781 attattctgg cttcacgtat gaccagtggg cagccaatga cgtcgattgg ctatgagctg
     3841 attgtgatct ccgcctgcgt tttaggtggc gtttctctga aaggcggcat cggaaaaatc
     3901 tcatatgtgg tggcgggtat cttaatttta ggcaccgtgg aaaacgccat gaacctgctt
     3961 aatatttctc ctttcgcaca gtacgtagtt cgcggcttaa tcctgctggc agcggtgatt
     4021 ttcgaccgtt acaagcaaaa agcgaaacgc actgtctgat gcttttttcc acaacaattt
     4081 agcgtttttt cccaccacag ccagccgcca caacggttgg ctgttcttcg ttgcaaatgg
     4141 cgacccccgt cacactgtct atacttacat gtctgtaaag cgcgttctgc gcaacacaat
     4201 aagaaaagag aaggaggaga accgggtgac agaaccgtta accgaaaccc ctgaactatc
     4261 cgcgaaatat gcctggtttt ttgatctcga tggaacgctg gcggaaatca aaccgcatcc
     4321 cgatcaggtc gtcgtgcctg acactattct gcaaggactt cagctactgg caaccgcaag
     4381 tgatggtgca ttggcattga tttcagggcg ctcaatggtg gagcttgacg cactggcaaa
     4441 accttatcgc ttcccgttag cgggcgtgca tggggcggag cgccgtgaca tcaatggtaa
     4501 aacgcatatc gtttatctgc cggatgcgat tgcgcgtgat attagcgtgc aactgcatac
     4561 ggtcatcgcc cagtatcccg gcgcggagct ggaggcgaaa gggatggctt ttgcgctgca
     4621 ttatcgccag gctccgcagc atgaagacgc attaatgaca ttagcgcaac gtattactca
     4681 gatctggcca caaatggcgt tacagcaggg aaagtgtgtt gtcgagatca aaccgaaagg
     4741 taccagtaaa ggtgaggcga ttgctgcttt tatgcaggaa gctcccttta tcgggcgaac
     4801 gcccgtattt ctgggcgatg atctgaccga tgaatctggc ttcgcagtcg ttaaccggct
     4861 gggcggaatg tcagtaaaaa ttggcacagg tgcaactcag gcatcatggc gactggcggg
     4921 cgtgccggat gtctggagct ggcttgaaat gataaccacc gcattacaac aaaaaagaga
     4981 aaataacagg agtgatgact atgagtcgtt tagtcgtagt atctaaccgg attgcaccac
     5041 cagacgagca cgccgccagt gccggtggcc ttgccgttgg catactgggg gcactgaaag
     5101 ccgcaggtgg actgtggttt ggctggagcg gtgaaacagg gaatgaggat cagccgctaa
     5161 aaaaggtgaa aaagggtaac attacgtggg cctcttttaa cctcagcgaa caggaccttg
     5221 acgaatacta caacaaattc tccaatgccg ttctctggcc cgcttttcat tatcggctcg
     5281 atctggtgca atttcagcgt cctgcctggg atggctatct acgcgtaaat gcgttgctgg
     5341 cagataaatt actgccgctg ttgcaagacg atgacattat ctggatccac gattatcacc
     5401 tgttgccctt tgcgcatgaa ttacgcaaac ggggcgtgaa taatcgcatt ggtttctttc
     5461 tgcatattcc tttcccgaca ccggaaatct tcaacgcgct gccgacatat gacaccttgc
     5521 ttgaacagct ttgtgattat gatttgctgg gtttccagac ggagaacgat cgtctggcgt
     5581 tcctggattg cctttctaac ctgacccgcg tcacgacacg tagcgcaaaa agccatacgg
     5641 cctgcggcaa agcgtttcga acggaagttt acccgatcgg cattgagccg aaagaaatag
     5701 cgaaacaggc tgccggtcca ctgccgccaa aactggcgca actcaaagca gaactgaaaa
     5761 acgtacaaaa tatcttttct gtcgaacgac tggattattc caaaggtttg ccggagcgtt
     5821 ttctcgccta tgaagcgtta ctggaaaaat atccgcaaca tcacggtaaa attcgttata
     5881 cccagatcgc gccaacgtct cgtggtgatg tgcaagccta tcaggatatt cgtcatcagc
     5941 tcgaaaatga agccggacga attaatggta aatatgggca attaggctgg acgccgcttt
     6001 attatttgaa tcagcatttt gaccgtaaat tactgatgaa aatattccgc tactctgacg
     6061 tgggcttagt gacgccactg cgtgacggga tgaacctggt ggcaaaagag tatgttgccg
     6121 ctcaggaccc agccaacccg ggcgttcttg ttctttcgca atttgcggga gcggcaaacg
     6181 agttaacgtc ggcgttaatt gttaatccct acgatcgtga cgaagttgca gctgcgctgg
     6241 atcgtgcatt gactatgtcg ctggcggaac gtatttcccg tcatgcagaa atgctggacg
     6301 ttatcgtgaa aaacgatatt aaccactggc aggagtgctt cattagcgac ctaaagcaga
     6361 tagttccgcg aagcgcggaa agccagcagc gcgataaagt tgctaccttt cctaagcttg
     6421 cgtaggtgcg gttaatctcc cgtaagtgga actaacagca catcgacctc acttgaggta
     6481 atcactcttt tcgccgagca ggatgctcgc gaaaagaaac tgtgattgtg attaccgcaa
     6541 atcaccaaat cgaaatgatg tttgcgacaa acttccagaa tatgttcgct taactcaccg
     6601 taggcaataa acgtcttgtc aacgggatac cccgcatcct gaattaactt atcaagaaag
     6661 ctttgcgttt cttcctgcat cacattgcgt aaatcttcaa gcatcggcgc agctaattga
     6721 ttgtacattt ccgggtcgga agcgagagta attaaactga tgtgtccctt tactggcctg
     6781 gcgatagata ccgcttttgc cagcagttgc tgactttccg gtgtaaccgc aacggcgaca
     6841 agaatattgc tatagctcat aacctgttcc ttattctgtg aacttcaggt gacattaaag
     6901 ctttactcct aatggagaaa cgacgcaatc ccaactcagt taaacaggta aatattag
//
LOCUS       DADSYM010000190         6897 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2415
            SAMN08039579-rid11723173.denovo.192, whole genome shotgun sequence.
ACCESSION   DADSYM010000190 DADSYM010000000
VERSION     DADSYM010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039579
            Sequence Read Archive: SRR6362533
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6897)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6897)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980162.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 03:39:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,484
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 4,347
            CDSs (with protein)               :: 4,347
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 43 of 85
            Pseudo Genes (incomplete)         :: 37 of 85
            Pseudo Genes (internal stop)      :: 19 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6897
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039579-rid11723173.denovo.192"
                     /strain="LGE2415"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8302 N 95.9628 W"
                     /collection_date="2015-08-24"
     gene            121..1518
                     /gene="ulaA"
                     /locus_tag="J1Y83_003986"
     CDS             121..1518
                     /gene="ulaA"
                     /locus_tag="J1Y83_003986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418614.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HBA7632084.1"
                     /translation="MEILYNIFTVFFNQVMTNAPLLLGIVTCLGYILLRKSVSVIIKG
                     TIKTIIGFMLLQAGSGILTSTFKPVVAKMSEVYGINGAISDTYASMMATIERMGDAYS
                     WVGYAVLLALALNICYVLLRRITGIRTIMLTGHIMFQQAGLIAVTLFIFGYSMWTTII
                     CTAILVSLYWGITSNMMYKPTQEVTDGCGFSIGHQQQFASWIAYKVAPFLGKKEESVE
                     DLKLPGWLNIFHDNIVSTAIVMTIFFGAILLSFGIDTVQAMAGKVNWTVYILQTGFSF
                     AVAIFIITQGVRMFVAELSEAFNGISQRLIPGAVLAIDCAAIYSFAPNAVVWGFMWGT
                     IGQLIAVGILVACGSSILIIPGFIPMFFSNATIGVFANHFGGWRAALKICLVMGMIEI
                     FGCVWAVKLTGMSAWMGMADWSILAPPMMQGFFSIGIAFMAVIIVIALAYMFFAGRAL
                     RAEEDAEKQLAEQSA"
     gene            1534..1839
                     /gene="ulaB"
                     /locus_tag="J1Y83_003987"
     CDS             1534..1839
                     /gene="ulaB"
                     /locus_tag="J1Y83_003987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIB"
                     /protein_id="HBA7632085.1"
                     /translation="MTVRILAVCGNGQGSSMIMKMKVDQFLTQSNIDHTVNSCAVGEY
                     KSELSGADIIIASTHIAGEITVTGNKYVVGVRNMLSPADFGPKLLEVIKEHFPQDVK"
     gene            1849..2313
                     /gene="ulaC"
                     /locus_tag="J1Y83_003988"
     CDS             1849..2313
                     /gene="ulaC"
                     /locus_tag="J1Y83_003988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIA"
                     /protein_id="HBA7632086.1"
                     /translation="MKLRDSLAENKSIRLQAEAETWQDAVKIGVDLLVAADVVEPRYY
                     QAILDAVEQHGPYFVLAPGLAMPHGRPEEGVKKTGFALVTLKKPLEFNHEDNDPVDIL
                     ITMAAVDANTHQEVGIMQIVNLFEDEENFDRLRACRTEQEVLDLIDRTNAAA"
     gene            2327..2977
                     /gene="ulaD"
                     /locus_tag="J1Y83_003989"
     CDS             2327..2977
                     /gene="ulaD"
                     /locus_tag="J1Y83_003989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HBA7632087.1"
                     /translation="MSLPMLQVALDNQTMDSAYETTRLIAEEVDIIEVGTILCVGEGV
                     RAVRDLKALYPHKIVLADAKIADAGKILSRMCFEANADWVTVICCADINTAKGALDVA
                     KEFNGDVQIELTGYWTWEQAQQWRDAGIQQVVYHRSRDAQAAGVAWGEADITAIKRLS
                     DMGFKVTVTGGLALEDLPLFKGIPIHVFIAGRSIRDAASPVEAARQFKRSIAELWG"
     gene            2987..3841
                     /locus_tag="J1Y83_003990"
     CDS             2987..3841
                     /locus_tag="J1Y83_003990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase UlaE"
                     /protein_id="HBA7632088.1"
                     /translation="MLSKQIPLGIYEKALPAGECWLERLQLAKTLGFDFVEMSVDETD
                     DRLSRLDWSREQRLALVNAIVETGVRVPSMCLSAHRRFPLGSEDDAVRAQGLEIMRKA
                     IQFAQDVGIRVIQLAGYDVYYQEANNETRRRFRDGLKESVEMASRAQVTLAMEIMDYP
                     LMNSISKALGYAHYLNNPWFQLYPDIGNLSAWDNDVQMELQAGIGHIVAVHVKDTKPG
                     VFKNVPFGEGVVDFERCFETLKQSGYCGPYLIEMWSETAEDPAAEVAKARDWVKARMA
                     KAGMVEAA"
     gene            3841..4527
                     /locus_tag="J1Y83_003991"
     CDS             3841..4527
                     /locus_tag="J1Y83_003991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase UlaF"
                     /protein_id="HBA7632089.1"
                     /translation="MQKLKQQVFEANMDLPRYGLVTFTWGNVSAIDRERGLVVIKPSG
                     VAYETMKADDMVVVDMSGKVVEGEYRPSSDTATHLELYRRYPSLGGIVHTHSTHATAW
                     AQAGLAIPALGTTHADYFFGDIPCTRGLSEEEVQGEYELNTGKVIIETLGDAEPLHTP
                     GIVVYQHGPFAWGKDAHDAVHNAVVMEEVAKMAWIARSINPQLNHIDSFLMNKHFMRK
                     HGPNAYYGQK"
     gene            complement(4656..4931)
                     /locus_tag="J1Y83_003992"
     CDS             complement(4656..4931)
                     /locus_tag="J1Y83_003992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HBA7632090.1"
                     /translation="MFSRVLALLAVLLLSANTWAAIEINNHQARNMDDVQSLGVIYIN
                     HNFATESEARQALNEETDAQGATYYHVILMREPGSNGNMHASADIYR"
     gene            5259..5654
                     /gene="rpsF"
                     /locus_tag="J1Y83_003993"
     CDS             5259..5654
                     /gene="rpsF"
                     /locus_tag="J1Y83_003993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020456251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HBA7632091.1"
                     /translation="MRHYEIVFMVHPDQSEQVPGMIERYTAAITGAEGKIHRLEDWGR
                     RQLAYPINKLHKAHYVLMNVEAPQEVIDELETTFRFNDAVIRSMVMRTKHAVTEASPM
                     VKAKDERRERRDDFANETADDAEAGDSEE"
     gene            5661..5975
                     /gene="priB"
                     /locus_tag="J1Y83_003994"
     CDS             5661..5975
                     /gene="priB"
                     /locus_tag="J1Y83_003994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001315977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N"
                     /protein_id="HBA7632092.1"
                     /translation="MTNRLVLSGTVCRTPLRKVSPSGIPHCQFVLEHRSVQEEAGFHR
                     QAWCQMPVIVSGHENQAITHSITVGSRITVQGFISCHKAKNGLSKMVLHAEQIELIDS
                     GD"
     gene            5980..6207
                     /gene="rpsR"
                     /locus_tag="J1Y83_003995"
     CDS             5980..6207
                     /gene="rpsR"
                     /locus_tag="J1Y83_003995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012986941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HBA7632093.1"
                     /translation="MARYFRRRKFCRFTAEGVQEIDYKDIATLKNYITESGKIVPSRI
                     TGTRAKYQRQLARAIKRARYLSLLPYTDRHQ"
     gene            6249..6698
                     /gene="rplI"
                     /locus_tag="J1Y83_003996"
     CDS             6249..6698
                     /gene="rplI"
                     /locus_tag="J1Y83_003996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HBA7632094.1"
                     /translation="MQVILLDKVANLGSLGDQVNVKAGYARNFLVPQGKAVPATKKNI
                     EFFEARRAELEAKLAEVLAAANARAEKINALETVTIASKAGDEGKLFGSIGTRDIADA
                     VTAAGVEVAKSEVRLPNGVLRTTGEHEVSFQVHSEVFAKVIVNVVAE"
BASE COUNT         1643 a         1716 c         1900 g         1638 t
ORIGIN      
        1 gcgtgcgacg tcacatttaa tcataaataa tcttgttgtg attacttttg aaaattagag
       61 tgagagcaca acattccggg tgtacgaaat acccggttac ctcttcttca ggagatcgtt
      121 atggagatcc tctacaacat ctttaccgtg ttttttaacc aggtcatgac caatgccccg
      181 ttgttgctgg gtattgtgac ctgtctgggt tacatcctac tgcgcaaaag tgtcagcgtt
      241 attattaaag gtacgattaa aaccataatt ggtttcatgt tgttgcaggc agggtccggc
      301 atcctgacca gcaccttcaa accggtggtg gcgaaaatgt ccgaagtcta cggcattaac
      361 ggcgcaattt ccgatactta cgcgtcgatg atggcaacca ttgaacgcat gggcgatgcc
      421 tatagctggg tgggttacgc ggtgttgtta gcgctggcgc tgaacatctg ttacgtgctg
      481 ctgcgtcgca ttaccggtat tcgtaccatc atgctgactg gtcacatcat gttccagcag
      541 gcggggctga ttgccgttac gctgtttatc ttcggctact ccatgtggac gaccattatc
      601 tgcaccgcga ttctggtttc gctctactgg ggcattacct ccaacatgat gtacaagccg
      661 acacaggaag tcaccgacgg ctgtggtttc tccattggtc accagcagca gttcgcatca
      721 tggattgctt ataaagtcgc accgttcctc ggcaaaaaag aggaaagcgt tgaagacctc
      781 aaactgcctg gctggctgaa tattttccac gacaacatcg tctccacggc gattgtcatg
      841 actatcttct ttggtgccat tctgctctcc tttggtatcg acaccgtgca ggcgatggca
      901 ggcaaagtga actggacggt gtacatcctg caaaccggtt tctcctttgc ggtggcgatt
      961 ttcatcatca ctcagggcgt gcgcatgttt gtggcggagc tctctgaagc attcaacggt
     1021 atctcccagc gcctgatccc gggtgcggtt ctggcgattg actgtgcggc tatttatagc
     1081 ttcgcgccga acgccgtggt ctggggtttt atgtggggca ccatcgggca actgattgcg
     1141 gttggcatcc tggtcgcctg tggctcttca atcctgatta ttccaggctt tatcccgatg
     1201 ttcttctcca acgccactat cggcgtgttc gctaaccact tcggcggctg gcgcgcggcg
     1261 ctgaagattt gcctggtgat ggggatgatc gaaatctttg gttgcgtctg ggcggtgaaa
     1321 ctcaccggta tgagcgcctg gatgggcatg gcggactggt cgattctggc accgccgatg
     1381 atgcagggct tcttctccat cggtatcgcc tttatggccg tcatcattgt cattgcactg
     1441 gcttatatgt tcttcgctgg ccgcgcactg cgtgcagaag aagatgcaga aaaacaactg
     1501 gcagaacaat ctgcttaata aggagttttg attatgaccg tacgtattct ggctgtgtgt
     1561 ggtaacggac aaggcagttc catgatcatg aaaatgaaag tggaccagtt tttaacccag
     1621 tcgaacatcg accacacggt aaacagctgc gcggttggcg aatacaaaag cgagttgagc
     1681 ggtgcggata tcatcatcgc ttctacccat attgcgggcg aaatcaccgt gaccggcaac
     1741 aaatatgtgg ttggcgtgcg caacatgctc tctcctgctg attttggccc gaaactgctg
     1801 gaagtgatca aagagcattt cccgcaggac gtgaagtaag gacgcgccat gaaattacgt
     1861 gattcgctgg ctgaaaataa atctatccgc ctgcaagcgg aagcagagac atggcaggac
     1921 gcagtgaaaa tcggcgttga tctgctggta gcggcagatg tggtagagcc gcgttactac
     1981 caggcgattc tggatgcggt tgaacagcac ggcccgtact ttgtgctcgc acccggcctg
     2041 gcaatgccgc acggacgccc ggaagagggg gttaagaaaa ctggttttgc gctggtgacg
     2101 ctgaaaaagc cgctggagtt caaccacgaa gataacgatc cggtggatat cctcatcacc
     2161 atggcggcgg ttgatgccaa cactcaccag gaagtgggca tcatgcagat cgttaacctg
     2221 tttgaagatg aagagaattt cgaccgttta cgcgcctgcc gtaccgagca ggaagtactg
     2281 gatttaattg accgcaccaa cgcggcagct taagaaggaa ttgaagatgt cattaccaat
     2341 gttgcaagtc gcgctggaca accagactat ggatagcgcc tacgaaacca ctcgcctgat
     2401 tgccgaagaa gtcgacatta tcgaagtggg caccattctg tgcgtgggcg aaggcgtgcg
     2461 tgcggttcgt gacctgaaag cgctttaccc gcacaaaatc gtactggcag acgccaaaat
     2521 tgccgatgcg ggcaaaatcc tttctcgtat gtgcttcgaa gctaacgctg actgggtgac
     2581 cgtgatttgc tgtgcggata tcaacaccgc caaaggtgcg ctggacgtgg caaaagagtt
     2641 taacggcgac gtgcagatcg aactgaccgg ttactggacc tgggaacagg cgcaacagtg
     2701 gcgcgatgca ggcattcagc aggtggttta tcaccgcagc cgtgacgcac aggccgcagg
     2761 cgtggcgtgg ggcgaagctg acattaccgc gatcaaacgt ctttccgata tgggcttcaa
     2821 agtcaccgtt actggtggcc tggcgctgga agatctgccg ctgttcaaag gcattccgat
     2881 tcatgtgttt atcgctggtc gcagcatccg tgatgccgct tctccggttg aagccgcgcg
     2941 tcagttcaaa cgttccatcg ccgaactgtg gggctaagga gcggatatgt tgtccaaaca
     3001 aattccgctt ggcatctatg aaaaagcgct ccccgccggg gagtgctggc tggaacggct
     3061 gcaactggca aaaacgttag gcttcgattt tgtcgagatg tcggtagatg aaactgacga
     3121 tcgcctgtcg cgcctcgact ggagccgcga gcagcgtctg gcgctggtga atgcgattgt
     3181 tgaaactggc gtgcgcgtgc cgtccatgtg cctttctgct catcgtcgtt tcccgctggg
     3241 cagcgaagat gacgcggtac gggcgcaggg gctggagatt atgcgcaaag ctatccagtt
     3301 cgcccaggac gtcggcattc gcgtgatcca actggcgggc tatgacgttt actatcagga
     3361 agccaataac gaaacgcgtc gtcgtttccg tgacggcctg aaagagagcg ttgagatggc
     3421 aagccgcgcg caggtgacgc tggcgatgga gatcatggat tacccgttga tgaactccat
     3481 cagtaaggcg ttgggttacg cgcactatct caacaaccca tggttccagc tctacccgga
     3541 tatcggcaac ctgtcggcgt gggacaacga cgtgcagatg gaattgcagg ccggaatcgg
     3601 gcatatcgtc gcggtgcatg tgaaagacac caaaccaggc gtcttcaaaa acgtgccgtt
     3661 tggcgaaggc gtagtggatt ttgaacgttg cttcgaaacg ctcaaacaga gtggctattg
     3721 cgggccgtac ctgattgaga tgtggagcga aacggcggaa gacccggcgg cggaagtggc
     3781 gaaagcgcgt gattgggtga aagcgcgcat ggccaaagct ggcatggtgg aggcggcata
     3841 atgcaaaagc tgaaacagca ggtatttgaa gccaacatgg atctgccgcg ctacgggctg
     3901 gtgaccttta cctggggcaa cgtcagcgcc atcgaccgcg aacgcgggct ggtggtgatc
     3961 aagcccagcg gcgtcgccta cgaaaccatg aaagcagacg atatggtggt ggtcgatatg
     4021 agcggcaagg tagtggaagg ggagtatcgc ccgtcttctg acaccgcgac gcatcttgaa
     4081 ctctaccgtc gttatccgtc gcttggtggt attgtccata cccactccac tcatgccacc
     4141 gcatgggcgc aggcggggct ggcgatcccg gcgttaggca ccacgcacgc cgattacttc
     4201 tttggcgaca tcccgtgcac gcgcggatta agcgaagaag aggtgcaggg tgagtacgaa
     4261 ctgaataccg gcaaagtaat tatcgaaacg ctgggtgacg ccgagccgct gcatacgccg
     4321 ggaattgtgg tgtatcagca cggaccgttc gcctggggga aagatgccca cgacgcggtg
     4381 cataacgcgg tggtgatgga agaagtggcg aaaatggcgt ggattgcgcg cagtattaat
     4441 ccacaactga atcacatcga cagcttcctg atgaataaac acttcatgcg taagcacggt
     4501 cccaacgcct attacgggca gaagtaaaac acgcgctgcg gaaatttcct tcttcgggag
     4561 ataactggtc taattccgca gccgtttttc aaaaaaagcc ccctgcgaag ggggcaaagc
     4621 aaactatggc aatgtttcgt tggttatacc tggtgctagc gataaatatc cgcgctggcg
     4681 tgcatattgc cgttactccc cggttcccgc atcagaatca catggtagta cgttgcgcct
     4741 tgcgcatctg tctcttcatt taatgcctga cgtgcttcac tctcagtggc gaagttatga
     4801 ttgatataaa tcacgcctaa gctttgcaca tcgtccatat ttctggcctg gtggttatta
     4861 atttcaatgg ctgcccatgt atttgcactt agcaaaagca cagccagaag ggctaaaacg
     4921 cgactgaaca tagatacctc ctcgacggct gactttgtgt gctctcctct tgtggtgatc
     4981 ttctgattta attttaatca atgataaaga agttgatggt gaccatttct gatgcagttg
     5041 ttcaaaaaaa caccatgacg aagtgtgatg aactccaaat cagcggttta gaggttattt
     5101 gcgaaagggg agatttattt cggctctgcc cttgagttta gcggggtatg tcagtactat
     5161 aacgcgtcat ttttcagccg acctttaaca cgttccttgc ctccccggga ttcggctgac
     5221 ccagacagga ggctgaataa tccgtaagga gcaattcgat gcgtcattac gaaatcgttt
     5281 ttatggtcca tcctgaccag agcgaacagg ttccgggcat gatcgagcgc tacactgctg
     5341 ccatcactgg tgcagaaggc aagatccacc gtctggaaga ctggggccgc cgtcagctgg
     5401 cttacccgat caacaaactg cacaaagcac actacgttct gatgaacgtt gaagctccgc
     5461 aggaagtgat cgatgagctg gaaactacct tccgcttcaa cgatgccgtt atccgcagca
     5521 tggttatgcg taccaagcac gctgttaccg aagcatctcc gatggttaaa gcgaaagacg
     5581 agcgccgtga gcgtcgcgat gatttcgcaa acgaaaccgc tgatgatgct gaagctgggg
     5641 attctgaaga gtaatttctg atgaccaacc gtctggtgtt gtccggcacc gtgtgcagga
     5701 ctccccttcg aaaggtcagt ccatcaggaa ttcctcactg ccagttcgtg cttgagcatc
     5761 gttctgtgca ggaggaagcc ggctttcacc ggcaggcgtg gtgccaaatg cccgttattg
     5821 ttagcggaca cgaaaaccag gccattactc acagtataac ggtcggcagt cgcataaccg
     5881 ttcaggggtt catttcatgc cataaggcaa agaacggact gagcaaaatg gttttgcatg
     5941 ccgagcagat tgaattgata gattctggag actagccata tggcacgtta tttccgtcgt
     6001 cgcaagttct gccgtttcac cgcggaaggc gttcaagaga tcgactataa agatatcgct
     6061 acgctgaaaa actacatcac cgaaagcggt aagattgtcc caagccgtat caccggtacc
     6121 cgtgcaaaat accagcgtca gctggctcgc gctatcaaac gcgctcgcta cctgtccctg
     6181 ctgccgtaca ctgatcgcca tcagtaatcg gtcacggtcc attaatacga ctttgagagg
     6241 ataaggtaat gcaagttatt ctgcttgata aagtagcaaa cctgggtagc ctgggtgatc
     6301 aggtaaacgt taaagcgggc tatgctcgta acttcctggt accgcagggt aaagctgttc
     6361 cagctaccaa gaaaaacatt gaattcttcg aagcacgtcg cgctgaactg gaagctaaac
     6421 tggctgaagt tctggcagca gctaatgctc gcgctgagaa aatcaatgca ctggaaactg
     6481 ttaccatcgc gtctaaagct ggcgacgaag gtaaactgtt cggttccatc ggtactcgcg
     6541 acatcgctga cgctgtaact gcagctggcg ttgaagtggc taagagcgaa gttcgtctgc
     6601 cgaacggcgt tctgcgtacc actggcgaac acgaagtgag cttccaggtt cacagcgaag
     6661 tattcgcgaa agtgatcgta aacgtagtag ctgaataatt cgttattcaa cgagacgtaa
     6721 aaacgccgac cagtggtcgg cgttttgctt ttaagcgata aaaaccgcag aaatagacgt
     6781 tccctccata aatctcttgc tattcctttt ataaaaagtt cgcagtaccc atacctggcc
     6841 cccagcctcg ccgctggtag ttcgtgtcat gttttttacg cgtaaaacac gacaaaa
//
LOCUS       DADSYM010000191         6721 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2415
            SAMN08039579-rid11723173.denovo.193, whole genome shotgun sequence.
ACCESSION   DADSYM010000191 DADSYM010000000
VERSION     DADSYM010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039579
            Sequence Read Archive: SRR6362533
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6721)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6721)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980162.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 03:39:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,484
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 4,347
            CDSs (with protein)               :: 4,347
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 43 of 85
            Pseudo Genes (incomplete)         :: 37 of 85
            Pseudo Genes (internal stop)      :: 19 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6721
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039579-rid11723173.denovo.193"
                     /strain="LGE2415"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8302 N 95.9628 W"
                     /collection_date="2015-08-24"
     gene            <1..430
                     /gene="osmC"
                     /locus_tag="J1Y83_003997"
     CDS             <1..430
                     /gene="osmC"
                     /locus_tag="J1Y83_003997"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="peroxiredoxin OsmC"
                     /protein_id="HBA7632095.1"
                     /translation="TIHKKGQAHWEGDIKRGKGTVSTESAVLNQQPYGFNTRFEGEKG
                     TNPEELIGAAHAACFSMALSLMLGEAGFTPTSIDTTADVSLDKVDAGFAITKIALKSE
                     VAVPGIDASTFDGIIQKAKAGCPVSQVLKAEITLDYQLKS"
     gene            complement(620..3019)
                     /gene="dosP"
                     /locus_tag="J1Y83_003998"
                     /gene_synonym="pdeO"
     CDS             complement(620..3019)
                     /gene="dosP"
                     /locus_tag="J1Y83_003998"
                     /gene_synonym="pdeO"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-sensing cyclic-di-GMP phosphodiesterase"
                     /protein_id="HBA7632096.1"
                     /translation="MKLTDADNAADGIFFPALEQNMMGAVLINENDEVMFFNPAAEKL
                     WGYKREEVIGNNIDMLIPRDLRPAHPQYIRHNREGGKARVEGMSRELQLEKKDGSKIW
                     TRFALSKVSAEGKVYYLALVRDASVEMAQKEQTRQLIIAVDHLDRPVIVLDPERHIVQ
                     CNRAFTEMFGYCINEASGMQPDTLLNIPEFPADNRIRLQQLLWKTARDQDEFLLLTRT
                     GEKIWIKASISPVYDVLAHLQNLVMTFSDITEERQIRQLEGNILAAMCSSPPFHEMGE
                     IICRNIESVLNESHVSLFALRNGMPIHWASSSHGAEVQNAQSWSATIRQRDGAPAGIL
                     QIKTSSGAETSAFIERVADISQHMAALALEQEKSRQHIEQLIQFDPMTGLPNRNNLHN
                     YLDDLVDKAVSPVVYLIGVDHIQDVIDSLGYAWADQALLEVVNRFREKLKPDQYLCRI
                     EGASFVLVSLENDVSNITQIADELRNVVSKPIMIDDKPFPLTLSIGISYDEGKNRDYL
                     LSTAHNAMDFIRKNGGNGWQFFSPAMNEMVKERLVLGAALKEAISNNQLKLVYQPQIF
                     AETGELYGIEALARWYDPLHGHVPPSRFIPLAEEIGEIENIGRWVIAEACRQLAEWRS
                     QNIHIPALSVNLSALHFRSNQLPNQVSDAMHAWGIDGHQLTVEITESMMMEHDTEIFK
                     RIQILRDMGIGLSVDDFGTGFSGLSRLVSLPVTEIKIDKSFVDRCLTEKRILALLEAI
                     TSIGQSLNLTVVAEGIETKEQFEMLRKIHCRVIQGYFFSRPLPAEEIPGWMSSVLPLK
                     I"
     gene            complement(3044..3262)
                     /locus_tag="J1Y83_003999"
     CDS             complement(3044..3262)
                     /locus_tag="J1Y83_003999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000335880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HBA7632097.1"
                     /translation="MCLVDFGLRFNHKGRHYFKAANGEDIALSLSIGAAMFNGHPDYE
                     RLIQIADEALYIAKRRGRNRVELCKASL"
     gene            complement(3586..4905)
                     /locus_tag="J1Y83_004000"
     CDS             complement(3586..4905)
                     /locus_tag="J1Y83_004000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 10 glycosylhydrolase"
                     /protein_id="HBA7632098.1"
                     /translation="MDICSRNEKLAIRRPAILVALALLLCSCKSTPPESMVTPPAGSK
                     PPAMTQQSSQPMRGIWLATVSRLDWPPVSSVNISNPTSRARVQQQAMIDKLDHLQRLG
                     INTVFFQVKPDGTALWPSKILPWSDLMTGKIGENPGYDPLQFMLDEAHKRGMKVHAWF
                     NPYRVSVNTKPGTIRELNSTLSQQPASVYVQHRDWIRTSGDRFVLDPGIPEVQDWITS
                     IVAEVVSRYPVDGVQFDDYFYTESPASRLNDNETYRKYGGAFASKADWRRNNTQQLIA
                     KVSHTIKSIKPEVEFGVSPAGVWRNRSHDPLGSDTRGAAAYDESYADTRRWVEQGLLD
                     YIAPQIYWPFSRSAARYDVLAKWWADVVKPTRTRLYIGIAFYKVGEPSKIEPDWMING
                     GVPELKKQLDLNDAVPEISGTILFREDYLNKPQTQQAVSYLQSRWGS"
     gene            complement(5036..6571)
                     /gene="gadC"
                     /locus_tag="J1Y83_004001"
     CDS             complement(5036..6571)
                     /gene="gadC"
                     /locus_tag="J1Y83_004001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance gamma-aminobutyrate antiporter
                     GadC"
                     /protein_id="HBA7632099.1"
                     /translation="MATSVQTGKAKQLTLLGFFAITASMVMAVYEYPTFATSGFSLVF
                     FLLLGGILWFIPVGLCAAEMATVDGWEEGGVFAWVSNTLGPRWGFAAISFGYLQIAIG
                     FIPMLYFVLGALSYILKWPALNEDPITKTIAALIILWALALTQFGGTKYTARIAKVGF
                     FAGILLPAFILIALAAIYLHSGAPVAIEMDAKTFFPDFSKVGTLVVFVAFILSYMGVE
                     ASATHVNEMSNPGRDYPLAMLLLMVAAICLSSVGGLSIAMVIPGNEINLSAGVMQTFT
                     VLMSHVAPEIEWTVRVISALLLLGVLAEIASWIVGPSRGMYVTAQKNLLPAAFAKMNK
                     NGVPVTLVISQLVITSIALIILTNTGGGNNMSFLIALALTVVIYLCAYFMLFIGYIVL
                     VLKHPDLKRTFNIPGGKGVKLVVAIVGLLTSIMAFIVSFLPPDNIQGDSTDMYVELLV
                     VSFLVVLALPFILYAVHDRKSKANTGVTLEPINSQNAPKGHFFLHPRARSPHYIVMND
                     KKH"
BASE COUNT         1745 a         1632 c         1662 g         1682 t
ORIGIN      
        1 gacaatccat aagaaaggtc aggcacactg ggaaggcgat atcaaacgcg ggaagggaac
       61 agtatctact gagagcgccg tgctgaacca acaaccgtat ggatttaaca cgcgttttga
      121 aggcgaaaaa ggaaccaacc ctgaagaact gattggcgca gcgcatgccg catgtttctc
      181 aatggcgctt tcattaatgc tgggggaagc gggattcacg ccaacatcga ttgataccac
      241 cgccgatgtc tcgctggata aagtggatgc cggttttgcg attactaaaa tcgcactgaa
      301 gagcgaagtt gcggtgccgg gtattgatgc ctccaccttt gacggcatta tccagaaagc
      361 aaaagcagga tgcccggttt ctcaggtact gaaagcggaa attacgctgg attaccagtt
      421 gaaatcgtaa agcgttgccg gatgacgcgt caggcgcgtg aatgcctgat gcgtcagcac
      481 aatcacatca ggctgagaag ggattgttct gacatggcta tgcgttgtcg ttgtccaact
      541 aaacgtagcg gaaatcacag tatttaagtg acagtgtcac gttaaataaa aacccgcgag
      601 tgcgggcgag aggaatttgt cagattttca gcggtaacac gctgctcatc cagcctggaa
      661 tttcttcggc gggcaggggg cgggaaaaga aatatccctg aataacgcga cagtggatct
      721 tgcgtagcat ctcaaattgc tctttggttt cgatgccttc cgccacgacg gttaaattga
      781 ggctttgccc aatgctggta atggcttcga gtaaggcaag gatgcgtttt tcggtcagac
      841 aacgatcgac aaaactttta tcaattttga tttccgttac cggaagactg actaagcggg
      901 ataatccgga aaagcctgta ccaaaatcat ctaccgataa accgatgccc atatcgcgca
      961 ggatctgaat gcgcttaaag atttcggtat cgtgttccat catcatgctt tccgtgattt
     1021 ccaccgtcag ctggtggccg tcaatacccc aggcgtgcat tgcatcagat acctgattag
     1081 gcagctgatt actgcgaaag tgcagcgcag acaagttaac ggataacgcc gggatatgaa
     1141 tattctggct acgccattct gctaactgac ggcaagcttc cgcgatgacc cagcgcccaa
     1201 tattttcgat ttcaccaatc tcttctgcga gaggaataaa ccgtgaaggg ggcacatgac
     1261 catgcagggg atcgtaccag cgagcaaggg cttcgatgcc gtacagttca cccgtttctg
     1321 cgaagatttg cggctgataa accagtttaa gttggttatt actaatcgct tcttttagcg
     1381 ctgcgcctaa aaccaaacgc tcttttacca tttcgttcat cgccgggctg aagaactgcc
     1441 agccgttacc gccatttttg cgaataaaat ccattgcatt gtgagcagtg gagagcaggt
     1501 aatcgcggtt tttaccctcg tcgtagctga tgccaatgct caaggtgagc gggaatggtt
     1561 tatcgtcaat cattatcggc ttgctgacca cattccgtag ctcatcggcg atctgggtaa
     1621 tgttactgac atcgttttcc aggctcacga ggacaaaaga cgcaccttca atacgacaga
     1681 gatactgatc cggtttgagt ttttcacgaa agcgattgac cacttccagc aatgcctgat
     1741 cggcccacgc atagccaagg ctatcaatca catcctgaat atggtcaaca ccgatgagat
     1801 acaccaccgg agagacggct ttgtcgacca agtcatcgag gtaattgtgc aggttattgc
     1861 gatttggcag accggtcatc ggatcaaatt ggatgagttg ttcaatatgc tgacggcttt
     1921 tttcctgttc cagcgccagc gcggccatat gctggctgat atctgccacg cgttcgataa
     1981 aggcgctggt ttctgctcct gacgaggttt taatttgcag gatccccgca ggcgcgccat
     2041 cgcgctgacg aatggtcgct gaccagcttt gcgcattttg aacttctgca ccgtgcgaag
     2101 atgacgccca gtgtatcggc atcccgttgc gcagcgcgaa cagcgaaaca tgcgattcgt
     2161 tgagaacaga ttcgatgtta cgacaaatga tttcccccat ttcatgaaat ggtgggctgc
     2221 tgcacatggc ggcgagaata ttgccttcaa gctggcggat ttgcctttct tcggtgatat
     2281 ccgagaatgt cattaccagg ttctgcagat gcgcgagcac gtcataaacc gggctgatag
     2341 aggctttaat ccagattttt tcaccggtgc gcgtcaacag cagaaattcg tcctgatcgc
     2401 gggcggtttt ccatagcaac tgctgtaaac gaatacggtt atcagcgggg aattctggaa
     2461 tgttcaggag tgtatcgggc tgcataccgc tggcttcgtt aatgcagtaa ccaaacattt
     2521 cggtaaatgc gcgattgcac tgcacaatat ggcgttccgg atcgaggaca atcaccggtc
     2581 ggtcgagatg gtcaacggca ataatcaatt gtcgggtctg ttctttttgc gccatttcta
     2641 cgctggcatc ccgtaccagc gccaggtaat aaactttccc ctcggcgctc actttcgata
     2701 gcgcaaaacg ggtccagatt ttactgccgt cttttttctc cagctgcagc tcccgactca
     2761 tcccctcgac gcgcgcttta ccaccttcac ggttgtgacg aatgtattga ggatgcgcag
     2821 gacgcaaatc ccgcggaatc agcatatcaa tgttattgcc aatgacttct tcacgtttgt
     2881 acccccagag cttctctgcg gcggggttga aaaacatcac ttcatcattt tcgttaatta
     2941 acaccgcgcc catcatattt tgctcaaggg cggggaaaaa aatgccatcg gcggcattat
     3001 ccgcatcggt tagcttcatg attacctctg catcctggcg tatctaaaga ctggctttac
     3061 agagttcaac acggtttcta cctcgtcttt tggcgatata cagagcttca tcggctattt
     3121 gaatgaggcg ctcatagtca ggatgaccat taaacatggc ggcaccgatg gaaagtgaga
     3181 gggcaatatc ctcgccgttt gcggctttaa aataatgtcg acctttatgg ttaaaccgca
     3241 ggccaaaatc aaccaaacac aacgtgcgag atcgactaac tgcaccatat tctcctgaaa
     3301 tatgaagata tactgaaaag aaataagcga cttaggacag tttcaatcta cgctactgtt
     3361 cttcagaaga gtatagctca tcgtaattat ttttcggtga cagcgaatat cgtatgtttt
     3421 ttcatattta tacattttta ttaggggaat tatggctgtt taactaagtg tggttaattg
     3481 cgtggttaat ttgacgtatt taagcatgat tgctagtggg atagttcaag agggtaacaa
     3541 gccagtgggt aaagcaccgg cttgttacaa agtaagaatg ggagtttaac tgccccaacg
     3601 actttgcaga tagctgaccg cttgttgagt ctgcggttta ttcagatagt cctcacggaa
     3661 caagattgtg ccgctaattt ccggcacagc atcgttaaga tcgagctgct ttttcagttc
     3721 tggtacgccg ccgttaatca tccagtctgg ctctatcttt gaaggttctc ctactttata
     3781 gaaggcgata ccgatataca ggcgggtcct ggtcggttta acgacatccg cccaccattt
     3841 tgccaacacg tcataacgcg cggcactccg tgagaatggc cagtaaattt ggggagcaat
     3901 gtaatccagc aacccttgtt ccacccatcg acgggtatca gcgtaggatt cgtcataggc
     3961 tgccgcacct cgggtatcgg aaccgagcgg atcgtgtgat cggttacgcc acacgcctgc
     4021 cgggctaacg ccaaattcga cttccggctt aatgctttta atggtgtgcg aaaccttcgc
     4081 aattaactgc tgggtattgt tgcgccgcca gtctgctttt gacgcaaatg cgccgccgta
     4141 tttgcggtac gtttcgttat catttagccg tgaagctggt gactcagtat agaaatagtc
     4201 atcaaactgc acgccatcta ccggataacg ggaaaccact tccgcgacta ttgatgtgat
     4261 ccagtcctga acctcaggga tgcccgggtc gaggacaaag cgatcgccgg acgttctgat
     4321 ccagtcgcgg tgttgcacat agacgctcgc cggttgttga gacagagtgc tattcagttc
     4381 cctgatagta ccgggcttcg tattaaccga tacgcgatag gggttaaacc aggcgtgtac
     4441 tttcatccca cgcttgtggg cttcgtcgag cataaattgc agcggatcgt aacccggatt
     4501 ttcaccaatc ttaccggtca taagatcgga ccacggcaaa attttcgatg gccacagggc
     4561 ggtaccatcc ggcttgacct ggaaaaagac cgtgtttatg ccgagacgtt gcagatgatc
     4621 cagtttgtcg atcatcgcct gttgttgtac acgtgcccgg ctggtggggt tactaatgtt
     4681 aaccgaggaa accggtggcc agtcgaggcg agacaccgtg gccagccaga tgccacgcat
     4741 cggttgtgac gattgttgcg tcatggctgg tggctttgaa cccgctggtg gtgtcaccat
     4801 ggactctgga ggcgtgcttt tacaactaca cagtaaaagt gccagtgcaa ccagtatcgc
     4861 tggtcttcta atcgctaatt tctcgtttcg ggagcagata tccatatgtg ctggtttccg
     4921 gtaaacagat gtgcgctcat tctcgtactt atcccggtta agtcaatacg acaacaagta
     4981 cgaaaaaggg agcgatgaat tatcgctccc ttgtcttata accattcaga catggttagt
     5041 gtttcttgtc attcatcaca atatagtgtg gtgaacgtgc acgcgggtgc aggaagaagt
     5101 gaccttttgg tgcgttctga ctgttgattg gctccagagt gacgccggta ttcgctttgc
     5161 ttttacgatc atgaacagca tagagaataa agggcagggc aagtaccacc aggaaactca
     5221 cgaccagtaa ttcaacatac atatcagtag agtcaccctg gatgttatcc ggcggcagga
     5281 aggaaacaat aaacgccata attgaagtca gcagaccgac aatagccacg accagtttca
     5341 cccctttacc accagggata ttaaatgtgc gttttaagtc aggatgttta agaaccaaca
     5401 caatgtagcc aataaacagc atgaaataag cacacagata aatcaccacc gtcagcgcca
     5461 gtgcgatcag gaaggacatg ttgttaccgc caccggtatt ggtgaggatg atcaacgcga
     5521 tagaagtaat caccaactgc gaaatgacca gcgttaccgg tacgccattt ttgttcattt
     5581 tagcgaatgc cgctggcagc aggtttttct gcgctgtgac atacatcccg cgagaaggac
     5641 caacaatcca ggaagcgatt tccgccagaa cacccagcaa cagcagtgcg gagatcacgc
     5701 gaaccgtcca ctcaatttcc ggtgccacat gtgacatcag aacggtaaag gtttgcatta
     5761 cccctgcgga gaggttgatt tcattacccg gaatgaccat cgcaatagac aaaccgccaa
     5821 cagagcttaa gcagattgcc gccaccatca gcagtaacat agccagtgga tagtcgcgcc
     5881 cggggttgct catttcattg acgtgggttg cggaagcttc aacgcccata taactcaaaa
     5941 tgaaggcaac aaatacaacc agggtgccca ctttagagaa gtcagggaag aaggtcttcg
     6001 catccatttc gatagcaacg ggggcaccgg agtgcagata aatagccgct aatgcgatca
     6061 aaataaatgc aggtaacagg ataccggcga agaagccaac tttagcaatt cgcgccgtgt
     6121 atttcgtgcc accaaactgc gttaatgcca gcgcccaaag aatgatgagt gctgcaatag
     6181 ttttggtaat ggggtcttca ttcagcgctg gccatttcag gatgtaggag agtgccccta
     6241 acacgaaata gagcatcgga ataaaaccaa tggcgatttg cagatagcca aatgagatcg
     6301 ctgcaaatcc ccatctcggc cccagagtat ttgataccca ggcgaagacg ccaccttctt
     6361 cccagccgtc gacggtagcc atttccgcag cacaaagtcc cacgggaata aaccataaaa
     6421 tcccgcctaa tagcaggaag aagactaatg aaaagcccga tgttgcgaag gtagggtatt
     6481 cataaacagc cattaccatc gatgccgtta tggcaaaaaa tccgagtaat gtgagctgct
     6541 tagctttacc tgtctgtact gatgtagcca tattatcccc ctaaaacggt attcctgtcg
     6601 gaaccgcacc tgttttgttc tgaagcgtat tcagaacaat attttccgtt gctaatgcca
     6661 gtgaacagac tttggaaatt gtcccgaaac gggttcgttt cgggacaccg ttaccgttaa
     6721 a
//
LOCUS       DADSYM010000192         6346 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2415
            SAMN08039579-rid11723173.denovo.194, whole genome shotgun sequence.
ACCESSION   DADSYM010000192 DADSYM010000000
VERSION     DADSYM010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039579
            Sequence Read Archive: SRR6362533
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6346)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6346)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980162.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 03:39:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,484
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 4,347
            CDSs (with protein)               :: 4,347
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 43 of 85
            Pseudo Genes (incomplete)         :: 37 of 85
            Pseudo Genes (internal stop)      :: 19 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6346
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039579-rid11723173.denovo.194"
                     /strain="LGE2415"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8302 N 95.9628 W"
                     /collection_date="2015-08-24"
     gene            complement(323..398)
                     /locus_tag="J1Y83_004002"
     tRNA            complement(323..398)
                     /locus_tag="J1Y83_004002"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(363..365),aa:Asn,seq:gtt)
     gene            complement(499..2142)
                     /gene="yeeO"
                     /locus_tag="J1Y83_004003"
     CDS             complement(499..2142)
                     /gene="yeeO"
                     /locus_tag="J1Y83_004003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416491.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic metabolite efflux MATE transporter YeeO"
                     /protein_id="HBA7632100.1"
                     /translation="MLRHILTAKNILSNPIFKFPNCLPFLSTVCCICRQFVGENLCSF
                     ADSPSSFEMWFHFLQLRSALNISSALRQVVHGTRWHSKRKSYKVLFWREITPLAVPIF
                     MENACVLLMGVLSTFLVSWLGKDAMAGVGLADSFNMVIMAFFAAIDLGTTVVVAFSLG
                     KRDRRRARVATRQSLVIMTLFAVLLATLIHHFGEQIIDFVAGDATTEVKALALTYLEL
                     TVLSYPAAAITLIGSGALRGAGNTKIPLLINGSLNILNIIISGILIYGLFSWPGLGFV
                     GAGLGLTISRYIGAVAILWVLAIGFNPALRISLTSYFKPLNFSIIWEVMGIGIPASVE
                     SVLFTSGRLLTQMFVAGMGTSVIAGNFIAFSIAALINLPGSALGSASTIITGRRLGIG
                     QIAQAEIQLRHVFWLSTLGLTAIAWLTAPFAGVMASFYTQDPQVKHVVVILIWLNALF
                     MPIWSASWVLPAGFKGARDARYAMWVSMLSMWGCRVVVGYVLGIMLGWGVVGVWMGMF
                     ADWAVRAVLFYWRMITGRWLWKYPRPEPQKCEKKPVVSE"
     gene            2147..2222
                     /locus_tag="J1Y83_004004"
     tRNA            2147..2222
                     /locus_tag="J1Y83_004004"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2180..2182,aa:Asn,seq:gtt)
     gene            complement(2260..3210)
                     /gene="cbl"
                     /locus_tag="J1Y83_004005"
     CDS             complement(2260..3210)
                     /gene="cbl"
                     /locus_tag="J1Y83_004005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator Cbl"
                     /protein_id="HBA7632101.1"
                     /translation="MNFQQLKIIREAARQDYNLTEVANMLFTSQSGVSRHIRELEDEL
                     GIEIFVRRGKRLLGMTEPGKALLVIAERILNEASNVRRLADLFTNDTSGVLTIATTHT
                     QARYSLPEVIKAFRELFPEVRLELIQGTPQEIATLLQNGEADIGIASERLSNDPQLVA
                     FPWFRWHHSLLVPLDHPLTQITPLTLESIAKWPLITYRQGITGRSRIDDAFVRKGLLA
                     DIVLSAQDSDVIKTYVALGLGIGLVAEQSSGEQEEENLIRLDTRHLFDANTVWLGLKR
                     GQLQRNYVWRFLELCNAGLSVEDIKRQVMENSEEEIDYQI"
     gene            complement(3312..4229)
                     /gene="nac"
                     /locus_tag="J1Y83_004006"
     CDS             complement(3312..4229)
                     /gene="nac"
                     /locus_tag="J1Y83_004006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen assimilation transcriptional regulator"
                     /protein_id="HBA7632102.1"
                     /translation="MNFRRLKYFVKIVDIGSLTQAAEVLHIAQPALSQQVATLEGELN
                     QQLLIRTKRGVTPTDAGKILYTHARAILRQCEQAQLAVHNVGQSLSGQVSIGFAPGTA
                     ASSITMPLLQAVRAEFPEVVIYLHENSGAVLNEKLINHQLDMAVIYEHSPVAGVSSQA
                     LLKEDLFLVGTQDCPGQSVDVNAIAQMNLFLPSDYSAVRLRVDEAFSLRRLTAKVIGE
                     IESIATLTAAIASGMGVAVLPESAARSLCGAVNGWMSRITTPSMSLSLSLNLPARANL
                     SPQAQAVKELLMSVISSPVMEKRQWQLVS"
     gene            4556..4631
                     /locus_tag="J1Y83_004007"
     tRNA            4556..4631
                     /locus_tag="J1Y83_004007"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4589..4591,aa:Asn,seq:gtt)
     gene            complement(4690..5250)
                     /locus_tag="J1Y83_004008"
     CDS             complement(4690..5250)
                     /locus_tag="J1Y83_004008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000116120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HBA7632103.1"
                     /translation="MSVQENTINILHELESNALKIDDAQAAQFISQIRNARHIFFQGA
                     GRSGIAIRAFANRLLHLGFSVSVVGEISSPHTQPGDLLIIGSGSGETTSLKSLAQKAV
                     DSGVNVALVTMKADSTIGKLAQSVLVLPGSVKDDNNRVSGAFSQPMGSAFEQLCFITY
                     DAIVLELMSELGESSATMFTRHADLE"
     gene            complement(5265..6017)
                     /locus_tag="J1Y83_004009"
     CDS             complement(5265..6017)
                     /locus_tag="J1Y83_004009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000064391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HBA7632104.1"
                     /translation="MSNFAGKVVLVTGGAHGIGKGIAKQFADRGAHTVIVDYNEENGK
                     ATAAELTFGDVKSLFVKTDVSDPQALVEVREKVFTTFGRIDVLILNAGVAFANKVNNI
                     SFEEWNKTIAINLSGLFNTVKAFYDDFLTNKGSIVYISSGSALSGTGGGVSYPASKAG
                     GEGLIRGLAKELGPKGVNVNAIAPRLIDTGEMMRVNYPTQADLDAVLEKIPVRRLGTI
                     DDVANLAIFLADKDNSYIQGQTILMDGGRTIA"
     gene            complement(6113..>6346)
                     /locus_tag="J1Y83_004010"
     CDS             complement(6113..>6346)
                     /locus_tag="J1Y83_004010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001062775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HBA7632105.1"
                     /translation="INPGQPGQTFIHKSLEKIRNAKKMISGRDIQIEVDGGVNLDNVK
                     QVKEAGADLIVVGGALFNKQPHESYQALKIAVQ"
BASE COUNT         1814 a         1616 c         1280 g         1636 t
ORIGIN      
        1 aaattccagg ccttcttttg ctgattacag aaggcaaagt ggcaattacg tatagttccc
       61 cgataaggac gctacagcaa catcccgcat aaggcatttt tctctttatc tttgtacggt
      121 acttcacgga acgaagtttt tgaccttgcg aatcgtgatg tctgttggga ggcaatttgc
      181 tcactgaagc gtgagactcg attaaacgca cgaaacacag aaatcaaaaa acccggtcac
      241 ttttttacaa ggtaaccggg taaaaataat ttttattttt taactgtcct gagactcata
      301 gagatgtctc aaaactaaaa tttggctcct ctgactggac tcgaaccagt gacatacgga
      361 ttaacagtcc gccgttctac cgactgaact acagaggaat cgtgagaacg aggcgaatat
      421 tagcgatgcc catccacaat gtcaaagcct gttttttaaa tttgaaatcg tttgctgaaa
      481 taatctgcat tttgtcgttt attccgacac aactggcttt ttttcacact tttgcggttc
      541 gggtcgaggg tatttccaca gccaacgtcc agtaatcatt cgccagtaaa acagcacggc
      601 ccgcacagcc cagtcggcaa acattcccat ccagactcca accacacccc agccaagcat
      661 gattcccagt acataaccga ctacaacccg gcacccccac atgctcaaca tagaaaccca
      721 catggcgtaa cgagcatcac gagccccctt aaatccagcg ggtagcaccc atgaggcgga
      781 ccaaataggc ataaataaag catttagcca aatcagaatc acaacgacgt gtttaacctg
      841 tggatcctgg gtgtaaaacg atgccataac cccggcaaag ggagccgtta gccaggcgat
      901 ggccgttaat ccaagagtgg acagccagaa cacatgccgc aactgaatct ctgcttgcgc
      961 tatctgccct atccccaacc ttcggcctgt aatgatcgta gaagcagaac cgagcgcact
     1021 tccgggtaag ttgataagag ccgcaattga gaacgcgata aaatttccgg caataacgct
     1081 ggtccccatc ccggcaacga acatttgggt taataaccga ccactggtaa ataacactga
     1141 ttcgacactc gctggaatac caatccccat gacttcccag ataatgctaa aattcagcgg
     1201 cttaaaatag ctcgttaacg aaatccttag cgcaggatta aaaccaatcg ccagcaccca
     1261 caaaattgca actgcgccaa tataacgaga aatggttaaa cccaacccag caccgacaaa
     1321 tcccagtccc ggccaggaga aaaggccgta aatcaatatg ccgctaataa taatattaag
     1381 aatattcagg ctaccgttaa tcagtagcgg tattttcgta ttccctgcac cacgaagtgc
     1441 cccgctacca ataagagtga tggcagctgc tggataactg agtacggtca gctccagata
     1501 agtcaacgcc agtgctttaa cttctgtcgt ggcatcaccc gcgacgaaat caataatttg
     1561 ttcgccaaaa tgatgaatta gcgtagccaa cagtacggca aacaacgtca tgatcaccaa
     1621 tgactgccgc gtcgccaccc tcgctcgtcg tcgatcccgc ttaccgagac taaatgctac
     1681 aacgacagta gtaccaagat cgatagctgc aaaaaaagcc ataatgacca tattgaagct
     1741 gtccgccaac cccacgccgg ccatcgcatc ttttcccagc cagctgacca gaaaagtgct
     1801 caggaccccc atcaacagga cacaggcgtt ctccatgaag ataggaacag caagcggggt
     1861 tatctcgcgc cagaacaaca ctttgtagct cttgcgttta gagtgccagc gagtaccgtg
     1921 aacaacctgg cgtaaagcag aggagatatt caaagccgac cttaattgca gaaagtgaaa
     1981 ccacatttca aatgatgagg gagaatcagc aaagctgcaa agattttcgc caacaaattg
     2041 tctgcaaatg caacaaactg ttgatagaaa cggcaaacag ttgggaaatt taaaaatcgg
     2101 gtttgacaaa atatttttcg ccgttaagat gtgcctcaac agcgattcct ctgtagttca
     2161 gtcggtagaa cggcggactg ttaatccgta tgtcactggt tcgagtccag tcagaggagc
     2221 caaatttatg aaaagcccgc ttttatagcg ggctttttgc tatatctgat aatcaatttc
     2281 ctcttcactg ttttccatca cctggcgctt gatatcctca actgacagtc ctgcattaca
     2341 aagttccaga aagcgccaga catagttacg ctgaagttgt cctcgcttca gtcccaacca
     2401 gacagtatta gcatcgaaaa gatgccgcgt atccaggcgg attaaattct cttcctcttg
     2461 ttcgccgctg gattgttcgg caactaatcc gatcccaagc ccaagagcaa cataggtttt
     2521 aatgacatca gaatcctgcg cacttaatac aatatctgcc agcaaacctt tgcggacaaa
     2581 tgcgtcatca atacgtgagc gccccgtaat cccctgtcgg taagtaatta acggccactt
     2641 cgctattgat tccagcgtca acggtgtaat ttgcgtcaag ggatgatcga gtggaacaag
     2701 caaactatgg tgccaacgaa accacgggaa ggcgacgagc tgcgggtcat tactcaaacg
     2761 ctcgctggca ataccaatat cagcttcgcc attttgcaac aacgtcgcaa tttcctgtgg
     2821 cgtaccctgg attagctcga gccgaacctc cgggaaaagt tcgcgaaaag ctttaatgac
     2881 ctctggcaag ctataacgtg cctgagtatg cgtcgttgca atagtgagaa caccagacgt
     2941 atcgttggta aacaggtctg caagccgacg aacattactg gcttcattca gaatacgttc
     3001 tgcaatgacc agtaatgctt tgcccggttc agtcatgccc agcagtcgct tacctcgtcg
     3061 aacaaatatt tcgatgccaa gttcatcctc cagttcccga atatgacggc taacgcctga
     3121 ctgtgaggta aaaagcatat tcgcaacctc tgtcaggttg taatcctgac gtgcagcctc
     3181 gcggattatc tttagttgtt ggaaattcac ggtaaactcc gggcagttca gatatcccgt
     3241 tattgttaaa gtctaatgcc cggcataaca aataataaaa acccgcatct tattccatcc
     3301 cgatataacg cttagctcac caattgccac tgcctttttt ccatcactgg agaactaatc
     3361 actgacatta acaactcttt cactgcctgt gcctgtggtg acaagtttgc tctggcgggt
     3421 aaatttaagg acaaagagag actcattgaa ggagtggtaa tgcgtgacat ccacccattt
     3481 actgcgccac ataacgaacg cgcagccgat tcaggtaata ctgcaacgcc catgccgctg
     3541 gcaatcgctg cggtaagcgt ggcaatagac tcaatttcac caatgacttt tgccgtgagc
     3601 cgccgtaggg aaaaagcctc atcaacacga agtctaacag cgctgtaatc actggggaga
     3661 aaaaggttca tttgcgcaat agcattaaca tcaacgcttt gccccgggca atcttgagtt
     3721 cctaccagga aaagatcttc tttcagcaaa gcctgactgg ataccccagc cacaggggaa
     3781 tgctcgtaaa tcaccgccat atcgagttgg tgatttatca gtttttcgtt aagcactgca
     3841 ccactatttt catgaagata gataacgacc tccgggaatt cagcgcgaac cgcctgtaat
     3901 aagggcatgg tgatggatga cgcagcggtt cctggcgcaa agccaatcga gacttgcccc
     3961 gataatgact gaccaacgtt atgcaccgcc agttgggcct gctcacactg acgtaaaatg
     4021 gcccgtgcat gggtatagag aatttttccg gcgtctgttg gcgtaacgcc ccgttttgtg
     4081 cgaatcaaaa gctgttgatt taactcacct tccagtgtgg caacctgctg gctgagcgct
     4141 ggttgtgcga tatgcaatac ttcagcagcc tgggtcaggc taccaatatc tacaattttt
     4201 acgaagtatt tcaggcgtct gaagttcatg ttgcctccgg tttttaagaa tcggcccaag
     4261 tgccgccatt acttacaacc agaatgcaag atgcttgcca gttctatttt agcgttgatg
     4321 cgcaagctaa ccaactgtca aataagagat tatgatagat tcgtcattgg ccctttttat
     4381 cagcagtcgc attcacctgc cctataaaag gggggtatgc accacgatgg ttcattaccc
     4441 aataagattg aaagctcacc actttgttga aattgtcagc aaacaaacaa aaaaatgcat
     4501 ttcacccttt gacatcacca tgcactgcca ttaatatgcg ccccgttcac acgattcctc
     4561 tgtagttcag tcggtagaac ggcggactgt taatccgtat gtcactggtt cgagtccagt
     4621 cagaggagcc aaattcaaaa aagcctgctt tctagcaggc tttttgcttt ctaattacca
     4681 actctttggt cattccagat cagcgtgtcg ggtaaacatt gtggcactgc tttcacccag
     4741 ttcagacatc aactccagaa caattgcatc ataagtaata aagcagagtt gttcaaaagc
     4801 tgagcccatt ggttgcgaga aagcgcctga aacacgattg ttatcgtctt tcacacttcc
     4861 tggtagtacc aacacagatt gggctaattt accgatcgtc gaatctgctt tcatagtgac
     4921 cagagctacg tttactccgc tatcaactgc tttttgtgcc agacttttca aactggtagt
     4981 ttcaccactg cctgacccaa taattaatag atctccaggt tgggtatgcg gtgatgatat
     5041 ttctcccaca acgcttactg aaaaacctaa atgcagcaag cgattagcaa aagcgcgaat
     5101 agcaatgcca ctgcgcccag ctccctgaaa aaaaatatgt cgggcatttc tgatttgcga
     5161 aataaactgt gcagcctgag catcgtcaat cttcagtgca ttactctcta attcatgcag
     5221 aatattaata gtattttcct gaacactcat tttttctctc ccttttaagc aatagtccgg
     5281 ccaccatcca ttaaaatagt ttgtccctga atataagagt tatctttatc tgccaaaaat
     5341 attgccaggt tagccacatc atcgatagtc cccagacgac ggacgggtat tttttccagg
     5401 acagcgtcta aatcggcttg tgttggatag ttgacccgca tcatctctcc ggtgtctatt
     5461 aagcggggag caatcgcatt aacattgacg ccttttggtc ctaactcttt tgccagacca
     5521 cgaatcaatc cttcgcctcc cgctttactg gcaggataac tcactccgcc cccagtcccc
     5581 gaaagcgctg atcccgatga gatataaaca atcgatccct tatttgtcag gaaatcatca
     5641 taaaacgcct taacggtatt aaataatccc gataagttaa ttgcgatagt tttattccat
     5701 tcctcaaaag aaatattgtt aactttatta gcaaatgcca caccagcatt taatatcaac
     5761 acatcaatac gaccaaaggt agtaaatacc ttttcccgta cctccactaa tgcctgtgga
     5821 tccgagacat ctgttttaac aaacagcgac ttaacatcgc caaaagttaa ctctgctgca
     5881 gtggccttgc cgttctcttc gttatagtca acaataacag tatgtgcacc acgatcagca
     5941 aattgtttag ctatcccttt gccaataccg tgagcacctc cggtaactaa aaccacttta
     6001 cctgcaaaat tactcattac ttttctccat taaagacttt ttagttgagg ttttcctccc
     6061 gatatgaaat cgaaaacctc acttagctgg atgtaaaatt tttagattgt gatcactgaa
     6121 cagcgatttt taatgcctga taactttcat gaggctgttt gttaaataat gcaccgccaa
     6181 caacaataag gtcagccccc gcctctttaa cttgtttcac attatcaaga ttaacgccac
     6241 catccacttc aatttgaata tctctgccag atatcatttt tttggcatta cggattttct
     6301 ccagactttt atgaataaat gtctggcctg gttgcccggg gttaat
//
LOCUS       DADSYM010000193         6214 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2415
            SAMN08039579-rid11723173.denovo.195, whole genome shotgun sequence.
ACCESSION   DADSYM010000193 DADSYM010000000
VERSION     DADSYM010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039579
            Sequence Read Archive: SRR6362533
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6214)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6214)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980162.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 03:39:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,484
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 4,347
            CDSs (with protein)               :: 4,347
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 43 of 85
            Pseudo Genes (incomplete)         :: 37 of 85
            Pseudo Genes (internal stop)      :: 19 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6214
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039579-rid11723173.denovo.195"
                     /strain="LGE2415"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8302 N 95.9628 W"
                     /collection_date="2015-08-24"
     gene            33..218
                     /locus_tag="J1Y83_004011"
     CDS             33..218
                     /locus_tag="J1Y83_004011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA7632106.1"
                     /translation="MSRRYSLATITLVILFCGRPHCRKISVRQYYQKRVIFYPTTLQR
                     TAFKKYLFLIRDIIISS"
     gene            179..298
                     /locus_tag="J1Y83_004012"
     CDS             179..298
                     /locus_tag="J1Y83_004012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001131457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HBA7632107.1"
                     /translation="MPLSYTGYHHFIMMSLMSGEHNTFALSLQDESPGLVPAG"
     gene            333..676
                     /locus_tag="J1Y83_004013"
                     /pseudo
     CDS             333..676
                     /locus_tag="J1Y83_004013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311542.2"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
     gene            complement(1048..1314)
                     /gene="minE"
                     /locus_tag="J1Y83_004014"
     CDS             complement(1048..1314)
                     /gene="minE"
                     /locus_tag="J1Y83_004014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division topological specificity factor
                     MinE"
                     /protein_id="HBA7632108.1"
                     /translation="MALLDFFLSRKKNTANIAKERLQIIVAERRRSDAEPHYLPQLRK
                     DILEVICKYVQIDPEMVTVQLEQKDGDISILELNVTLPEAEELK"
     gene            complement(1318..2130)
                     /gene="minD"
                     /locus_tag="J1Y83_004015"
     CDS             complement(1318..2130)
                     /gene="minD"
                     /locus_tag="J1Y83_004015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020801817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinD"
                     /protein_id="HBA7632109.1"
                     /translation="MARIIVVTSGKGGVGKTTSSAAIATGLAQKGKKTVVIDFDIGLR
                     NLDLIMGCERRVVYDFVNVIQGDATLNQALIKDKRTENLYILPASQTRDKDALTREGV
                     AKVLDDLKAMDFEFIVCDSPAGIETGALMALYFADEAIITTNPEVSSVRDSDRILGIL
                     ASKSRRAENGEEPIKEHLLLTRYNPGRVSRGDMLSMEDVLEILRIKLVGVIPEDQSVL
                     RASNQGEPVILDINADAGKAYADTVERLLGEERPFRFIEEEKKGFLKRLFGG"
     gene            complement(2154..2849)
                     /gene="minC"
                     /locus_tag="J1Y83_004016"
     CDS             complement(2154..2849)
                     /gene="minC"
                     /locus_tag="J1Y83_004016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinC"
                     /protein_id="HBA7632110.1"
                     /translation="MSNTPIELKGSSFTLSVVHLHEAEPKVIHQALEDKIAQAPAFLK
                     HAPVVLNVSALEDPVNWSAMHKAVSATGLRVIGVSGCKDAQLKAEIEKMGLPILTEGK
                     EKAPRPAPAPQAPAQNTTPVTKTRLIDTPVRSGQRIYAPQCDLIVTSHVSAGAELIAD
                     GNIHVYGMMRGRALAGASGDRETQIFCTNLMAELVSIAGEYWLSDQIPAEFYGKAARL
                     QLVENALTVQPLN"
     gene            3369..3737
                     /locus_tag="J1Y83_004017"
     CDS             3369..3737
                     /locus_tag="J1Y83_004017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA7632111.1"
                     /translation="MNMMRIFYIGLSGVGMMFSSMASGNDAGGLQSPACGVVCDPYIC
                     VNSDGISPELTRKYLGEKAAENLQSVQGYDPSEFTFSNGVFCDVKEKLCRDDRYFGVN
                     GKRSGKINQTTTKMLFMCRE"
     gene            complement(3840..4241)
                     /locus_tag="J1Y83_004018"
     CDS             complement(3840..4241)
                     /locus_tag="J1Y83_004018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inhibitor of g-type lysozyme"
                     /protein_id="HBA7632112.1"
                     /translation="MKIKGISKAVLLLALLTSTSFAAGKNVNVEFRKGHSSAQYSGEI
                     KGYDYDTYTFYAKKGQKVHVSISNEGADTYLFGPGIDDSVDLSRYSPELDSHGQYSLP
                     ASGKYELRVLQTRNDARKNKTKKYNVDIQIK"
     gene            4483..4776
                     /locus_tag="J1Y83_004019"
     CDS             4483..4776
                     /locus_tag="J1Y83_004019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA7632113.1"
                     /translation="MFCVIYRSSKRDQTYLYVEKKDDFSRVPEELMKGFGQPQLAMIL
                     PLDGRKKLVNADIEKVKQALTEQGYYLQLPPPPEDLLKQHLSVMGQKTDDINK"
     gene            4848..5507
                     /locus_tag="J1Y83_004020"
     CDS             4848..5507
                     /locus_tag="J1Y83_004020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HBA7632114.1"
                     /translation="MYQHHNWQGALLDYPVSKVVCVGSNYAKHIKEMGSAVPEEPVLF
                     IKPETALCDLRQPLAIPSDFGSVHHEVELAVLIGATLRQATEEHVRKAIAGYGVALDL
                     TLRDVQGKMKKAGQPWEKAKAFDNSCPLSGFIPAAEFTGDPQNTTLGLSVNGEQRQQG
                     TTADMIHKIVPLIAYMSKFFTLKAGDVVLTGTPDGVGPLQSGDELTVTFDGHSLTTRV
                     L"
     gene            5584..6045
                     /locus_tag="J1Y83_004021"
     CDS             5584..6045
                     /locus_tag="J1Y83_004021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415699.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgN family cysteine cluster protein"
                     /protein_id="HBA7632115.1"
                     /translation="MAEHLMSDVPFWQSKTLDEMSDAEWESLCDGCGQCCLHKLMDED
                     TDEIYFTNVACRQLNIKTCQCRNYERRFEFEPDCIKLTRENLPTFEWLPMTCAYRLLA
                     EGKGLPAWHPLLTGSKAAMHGERISVRHIAVKESEVIDWQDHILNKPDWAQ"
BASE COUNT         1608 a         1363 c         1508 g         1735 t
ORIGIN      
        1 ctgatttatc aatattattg tgtgattgtt atgtgagcag aagatattca ttagcaacga
       61 ttacattagt cattttattt tgtggacggc ctcattgtcg aaaaataagc gtacgacagt
      121 attatcagaa aagagtgatt ttttatccaa caacacttca gcgcactgcg tttaaaaaat
      181 acctctttct tatacgggat atcatcattt catcatgatg tctttgatga gcggtgaaca
      241 caatacattt gcgctgtctc ttcaggatga atcccctggt ctggtgcctg cgggctgatg
      301 ttgcagcaga gctaaggtca cttagacgct actatcattt atccaatgca tggaatcgaa
      361 atcagtcgat acccgcagta tatatcgtga actgggcgca acgctgagtt acaacatgcg
      421 tctggggaac ggtatggaaa ttgaaccctg gctgaaggcg gctgtgcgca aagaatttgt
      481 cgatgataat cgagtgaaag tgaatagtga cggtaatttt atcaatgatt tgtcgggtag
      541 acgtggaata taccaggcag gtattaaagc ctcattcagc agtacgttaa gcgggcatct
      601 cggggtgggg tatagccgtg gtgccggtgt ggagtccccg tggaatgcgg tggccggtgt
      661 gaactggtcg ttctgaccat caacgattaa actgcgcttc ggagcagttt tcgtttaccg
      721 ggtgctaaaa gggaaaattc tgcggcaaaa aaagcccgcc agttacggcg ggaaacctca
      781 tactatggga gaacaatgaa taatgaaatt gcggggttat catctcccag gatatccacg
      841 ctaacaataa ggttatttac tcaaccaggc gtaaacattt tgttctgtgc gtgggaacag
      901 ccttaaggtg taaaggggga gggggaaata gcaatgaata gtatcagcaa gaatactcgc
      961 cgctttacca caacgtggat gagagggatg aaaaactcaa ggcagagatg actctgcctt
     1021 gaagataaat gcgcttttac agcgggctta tttcagctct tctgcttccg gtaaggtcac
     1081 gttcagctca agaatagaaa tatcgccatc tttttgctca agctgtacgg ttaccatctc
     1141 aggatcaatt tgtacatatt tacaaatgac ctcaagaata tctttacgca actgcggcag
     1201 ataatgcggt tctgcatcgc tgcgacggcg ttcagcaaca ataatctgca accgttcttt
     1261 tgcaatgttg gctgtgtttt tcttccgcga gagaaagaaa tcgagtaatg ccataattta
     1321 tcctccgaac aagcgtttga ggaagccttt cttctcttct tcaatgaagc ggaaaggacg
     1381 ttcttctccc aacagacgtt ctacggtatc tgcgtaggct ttacccgcat cggcgttaat
     1441 gtcgagaatg accggttcac cctggttaga ggcgcgcaat actgattgat cctctgggat
     1501 cacgccgacg agtttgatgc gcaggatctc cagcacatct tccatgctca gcatgtcacc
     1561 tctgcttacg cggcctgggt tatagcgcgt taacagcagg tgctctttaa tcggctcttc
     1621 gccattttct gcgcggcgtg atttcgacgc cagaatgcct aaaatacggt cagagtcgcg
     1681 tactgaggag acttccgggt tggtggtaat aatggcttcg tctgcaaaat agagtgccat
     1741 taacgcaccg gtttcaatcc ctgccgggga gtcacaaacg ataaattcaa aatccatcgc
     1801 tttcagatca tcaagaactt tggcgacccc ttcacgagtg agggcatctt tatcgcgagt
     1861 ttgcgatgcc ggaagaatat agagattttc agtacgttta tctttaatta acgcctggtt
     1921 tagcgttgca tcgccctgaa tgacgttgac gaaatcgtaa acgacccggc gttcacaacc
     1981 cataatcagg tcgagattac gcaggccgat atcaaaatct atcacgacag ttttctttcc
     2041 cttctgggcc aaaccagtgg cgatggccgc gctggaggtt gtcttaccaa cacccccttt
     2101 gcccgaagta acaacaataa tgcgtgccat agaaattcct tgttaaaaag ggatcaattt
     2161 aacggttgaa cggtcaaagc gttttcgact aactgcagtc gtgccgcttt gccataaaat
     2221 tctgctggga tttgatcact cagccagtat tcacctgcga tggacaccag ttccgccatc
     2281 aggttcgtac aaaatatttg cgtttcccgg tcaccacttg cccctgccag cgcacgaccg
     2341 cgcatcatgc catagacatg aatgttacca tcggcaatca attcggcccc tgcgctaacg
     2401 tggcttgtaa caatcagatc acattgtgga gcataaatac gctgaccgga acgcactggg
     2461 gtatcaatta aacgcgtttt tgtgaccggc gttgtatttt gcgctggagc ctgcggtgcg
     2521 ggagctggac gcggcgcttt ttcctttcct tccgtcagga taggcagccc catcttttca
     2581 atttcggctt taagttgcgc atctttgcag ccacttacgc caataacccg taaaccagtt
     2641 gccgaaaccg ccttatgcat cgccgaccag tttaccgggt cttccagtgc actgacgttg
     2701 agtacaacgg gggcatgttt taaaaatgcg ggggcctgag cgattttgtc ttccagcgcc
     2761 tgatgaataa ccttaggttc tgcctcatgc agatgaacca cagataaagt gaagctactg
     2821 cctttaagct cgattggcgt gtttgacatc ctggccttac tcaattagct attaatcatc
     2881 gccagcgcgc gatgatgttc cgaagactat aaggcatgtt atagtctgga ttatattgag
     2941 gcaagtcacc ctcccattta ttcagagtaa aagtctattc tgtgataaat ggtgctgatt
     3001 catagcttaa aaaataccct tgtcaatcaa cccattgccg tcgtactttt gattgttctt
     3061 atttacgctt cctttttccg caccctaatc gtgacagcat cttcagacct gtttaaatat
     3121 ccagtgttga ggcgagtgta ttttttcacc acgccaggct ttcttaactt tccatttttt
     3181 gcagcatcat taaagaacag atacccgtta tcttaatatc cgccacagaa ttgctctatc
     3241 atagaaaccg cagaagacgt ctgatacttc tgtcaggtca tcgtaactcg tccaggtcag
     3301 cggttttgat tacggattga tgatgaaatg agcataagtg ttgtacggtt ggattatcag
     3361 gatgtgctat gaatatgatg cgtatttttt atatcggatt gtcaggtgtg gggatgatgt
     3421 tctcatcaat ggcgtctgga aatgacgctg gcggacttca atctccggcg tgcggcgttg
     3481 tttgcgaccc gtatatttgt gttaactcag atggcatttc cccagagtta acaaggaaat
     3541 atctcggcga aaaagccgct gaaaacttac aatcagtaca aggttacgat cccagcgaat
     3601 ttacattctc taacggtgta ttttgcgatg ttaaagaaaa attatgtcgt gatgatcgtt
     3661 attttggtgt gaatggtaag cggagtggaa aaatcaatca aaccaccaca aagatgttat
     3721 ttatgtgtcg tgagtgatgt cttgtttaca ttatgttcta ttcgtcatta acctcttaag
     3781 cgacagagag gagtggggcg ttaacgttta tgacggggag agtccccgtc ggttgatatt
     3841 tacttaattt gaatatcgac gttatatttt ttagttttgt ttttacgggc gtcgtttcgg
     3901 gtttgcagca ctcgcagctc atatttgcca gaagcaggga gtgagtattg tccgtgacta
     3961 tcgagttctg gggaatatct ggataaatca acagaatcat cgattcctgg cccgaagagg
     4021 taagtatcag cgccttcatt agagatactc acgtgcactt tctgaccttt tttggcatag
     4081 aaggtatatg tatcgtaatc gtatcccttt atttcgccgg agtactgagc gctgctatgg
     4141 ccttttctga actcaacatt gacattctta cccgcagcaa agctcgtgga ggttagcagt
     4201 gcaagcaaca atacagcctt actgatgccc ttaattttca ttggtagtct cctgttcaga
     4261 atgagtacga tgaactgtta taatataaca atccttcacg ggagaattcc agtggctttc
     4321 ctgtgttcgg ttaaatcccg gcgcggcatt tttgccgggg ggattactta taaagggggc
     4381 atggactgaa caaaccgtag aaatctacag ctggctggct tgtggcgcgg gtttcattga
     4441 tgttagctta tgccaaaacc tggcatctta aagagtaaaa gtatgttttg tgtgatttat
     4501 cgaagcagca agcgtgatca gacctattta tatgtcgaaa aaaaagacga tttttcgcgt
     4561 gttcctgagg aactgatgaa aggttttggt cagcctcagt tagcgatgat tcttccgctg
     4621 gatgggcgga agaaactggt caatgccgat attgaaaagg ttaaacaggc attaaccgag
     4681 caaggttact atttgcagtt accgccacca cccgaagatt tgctgaagca acatctttcc
     4741 gtcatggggc aaaaaacaga cgacattaac aaataaccga tatccggcgg tggcattatc
     4801 tttgtcggcg cgggttttca tatccacgat aaggtgaggg gaacgttatg tatcaacatc
     4861 acaactggca aggtgcgctg ctggattatc cggtgagtaa agtagtctgt gttggcagta
     4921 actatgccaa acatattaaa gagatgggca gcgcagtacc cgaagagcca gtgctgttta
     4981 ttaaaccaga aacggcactg tgcgatctgc ggcagccatt ggcgatccca tccgatttcg
     5041 gttcagttca tcatgaagtc gaactggcgg tgttgattgg cgcgacgctg cgtcaggcta
     5101 cggaagagca tgtccgcaaa gccattgccg gttacggcgt ggcgctcgat ctgacattgc
     5161 gtgatgttca gggaaaaatg aagaaagccg ggcagccgtg ggaaaaggcc aaagcgtttg
     5221 ataactcttg tccgctttcc gggtttattc ccgcggcgga gtttaccggc gacccgcaaa
     5281 atacaacgct gggcctgagc gtaaacggcg agcaacgcca gcaaggtacg actgcggaca
     5341 tgatccataa aatcgttccg ctgatcgctt atatgagcaa gttttttacc ctcaaggccg
     5401 gtgacgttgt gctgacaggc acgcctgatg gtgtcggccc gttgcaaagc ggtgatgagc
     5461 tgacagtcac tttcgatggg cattctttga caactcgcgt tttgtaatac tttttgccgc
     5521 ctgaaagcgg cggcaaaact tgcatcgctg tgccagactg gttataaggt gcgttttaac
     5581 gtaatggcgg aacacctgat gagcgatgta cctttctggc aaagtaaaac cctggacgaa
     5641 atgagcgatg cggaatggga gtcgttgtgt gatggttgcg gtcagtgttg cctgcataaa
     5701 ctgatggatg aagacaccga cgaaatctac ttcactaacg tcgcctgtcg ccagctcaat
     5761 attaaaacct gtcaatgtcg gaactacgaa cgtcgtttcg agtttgaacc cgactgcatt
     5821 aaattaaccc gtgaaaatct gccaacattc gaatggctgc caatgacctg cgcttatcgt
     5881 ttgctggcgg aaggtaaagg tttacctgcg tggcatccgc tacttactgg ttcgaaagcg
     5941 gcaatgcatg gtgaacgtat ctctgtgcgt catatcgcag taaaagaatc agaagtgatt
     6001 gactggcagg atcatatctt aaataaacct gactgggcgc agtgataaat ttaatagatg
     6061 ttgatggggg ctttcttaat acacgtctgg atgctcgcca tttcgtagcc ctgtttttga
     6121 aagcgctctg tcataatgag agttactcga taccttatta tcaacaggta actctcaatc
     6181 tttaaaaaac gtctgtattt ttaaatgtga taaa
//
LOCUS       DADSYM010000194         6177 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2415
            SAMN08039579-rid11723173.denovo.196, whole genome shotgun sequence.
ACCESSION   DADSYM010000194 DADSYM010000000
VERSION     DADSYM010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039579
            Sequence Read Archive: SRR6362533
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6177)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6177)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980162.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 03:39:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,484
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 4,347
            CDSs (with protein)               :: 4,347
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 43 of 85
            Pseudo Genes (incomplete)         :: 37 of 85
            Pseudo Genes (internal stop)      :: 19 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6177
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039579-rid11723173.denovo.196"
                     /strain="LGE2415"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8302 N 95.9628 W"
                     /collection_date="2015-08-24"
     gene            121..1029
                     /locus_tag="J1Y83_004022"
     CDS             121..1029
                     /locus_tag="J1Y83_004022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prohibitin family protein"
                     /protein_id="HBA7632116.1"
                     /translation="MKAPVSLTSFRPQKSLAIAIGVLAVVVLPFLSYYTVNEGERGIL
                     LRYGKIVKVAEPGLGFKIPFMESVEKISTRNQAVVYQGLQAYSRDQQPAQMTVSVSFH
                     IKPSEAGAVYTTYNTIEALKDRLIVRQLPTQLENVFGQYTAISAVQDRTKLVQDLQNA
                     MRKAVVGPVVIDGVQIENIDFSDAYEKSIEDRMKAEVAIATRKQNLETEKIQAQIAVT
                     QAQAEADSKLAAAKAEAETIRVRGAAEAETIRLKSAAEAEAIRLRGEALRENPGLVAL
                     TTAERWDGKLPDTMIPGSTVPFISTK"
     gene            complement(1249..3240)
                     /gene="tkt"
                     /locus_tag="J1Y83_004023"
     CDS             complement(1249..3240)
                     /gene="tkt"
                     /locus_tag="J1Y83_004023"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708699.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HBA7632117.1"
                     /translation="MSSRKELANAIRALSMDAVQKAKSGHPGAPMGMADIAEVLWRDF
                     LKHNPQNPSWADRDRFVLSNGHGSMLIYSLLHLTGYDLPMEELKNFRQLHSKTPGHPE
                     VGYTAGVETTTGPLGQGIANAVGMAIAEKTLAAQFNRPGHDIVDHYTYAFMGDGCMME
                     GISHEVCSLAGTLKLGKLIAFYDDNGISIDGHVEGWFTDDTAMRFEAYGWHVIRDIDG
                     HDAASIKRAIEEARAVTDKPSLLMCKTIIGFGSPNKAGTHDSHGAPLGDAEIALTREQ
                     LGWKYAPFEIPSEIYAQWDAKEAGQAKESAWNEKFAAYAKAYPQEAAEFTRRMKGEMP
                     SDFDAKAKEFIAKLQANPAKIASRKASQNAIEAFGPLLPEFLGGSADLAPSNLTLWSG
                     SKAINEDAAGNYIHYGVREFGMTAIANGISLHGGFLPYTSTFLMFVEYARNAVRMAAL
                     MKQRQVMVYTHDSIGLGEDGPTHQPVEQIASLRVTPNMSTWRPCDQVESAVAWKYGVE
                     RQDGPTALILSRQNLAQQERTEEQLANIARGGYVLKDCAGQPELIFIATGSEVELAVA
                     AYEKLTAEGVKARVVSMPSTDAFDKQDAAYRESVLPKAVTARVAVEAGIADYWYKYVG
                     LNGAIVGMTTFGESAPAEQLFEEFGFTVDNVVAKAKALL"
     gene            3518..4276
                     /locus_tag="J1Y83_004024"
     CDS             3518..4276
                     /locus_tag="J1Y83_004024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HBA7632118.1"
                     /translation="MKIRALLVAMSVATVLTGCQNMDSSGLLSSGAEAFQAYSLSDAQ
                     VKKLSDQACQEMDSKATIAPANSEYARRLTTISRALGDNINGQPVNYKVYMAKDVNAF
                     AMANGCIRVYSGLMDMMTDNEVEAVLGHEMGHVALGHVKKGMQVALGTNAIRVAAASA
                     GGIVGSLSQSQLGDLGEKLVNSQFTQRQESEADDYSYDLLRQRGISPAGLATSFEKLA
                     KLEEGRQSSMFDDHPASAERAQHIRDRMSADGVK"
     gene            complement(4367..5287)
                     /gene="speB"
                     /locus_tag="J1Y83_004025"
     CDS             complement(4367..5287)
                     /gene="speB"
                     /locus_tag="J1Y83_004025"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HBA7632119.1"
                     /translation="MSTLGHQYDNSLVSNAFGFLRLPMNFQPYDSDADWVITGVPFDM
                     ATSGRAGGRHGPAAIRQVSTNLAWEHNRFPWNFDMRERLNVVDCGDLVYAFGDAREMS
                     EKLQAHAEKLLAAGKRMLSFGGDHFVTLPLLRAHAKHFGKMALVHFDAHTDTYANGCE
                     FDHGTMFYTAPKEGLIDPNHSVQIGIRTEFDKDNGFTVLDACQVNDRSVDDVIAQVKQ
                     IVGDMPVYLTFDIDCLDPAFAPGTGTPVIGGLTSDRAIKLVRGLKDLNIVGMDVVEVA
                     PAYDQSEITALAAATLALEMLYIQAAKKGE"
     gene            complement(5423..6155)
                     /locus_tag="J1Y83_004026"
                     /pseudo
     CDS             complement(5423..6155)
                     /locus_tag="J1Y83_004026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311840.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         1515 a         1642 c         1596 g         1424 t
ORIGIN      
        1 acttattacg tttattattt tttcgctcat cccctttata aattctgaat tatcggctgt
       61 tgtctgtttt ctcatcaccc catgtgacat attcctatac tttactcaag gaagattcat
      121 atgaaagcac ctgtttcatt aacctcattt cgcccacaaa aatctcttgc tatcgccatt
      181 ggcgtgcttg ccgtggttgt gctgccattt ctctcgtatt acaccgttaa tgaaggtgaa
      241 cgcggcattt tgctgcgcta cggaaaaatc gtaaaagttg ccgagccggg gctgggtttt
      301 aagatccctt ttatggaaag cgtagagaaa atttccacgc gtaatcaggc tgtggtctat
      361 cagggattac aggcctacag ccgcgaccag caaccggcac aaatgacagt atcagtcagc
      421 ttccacatta aaccttcaga agcaggcgcg gtttatacca cctacaacac cattgaagca
      481 ttaaaagatc gtctgattgt ccgtcagttg ccaactcaac tggagaacgt tttcggacaa
      541 tataccgcca tcagcgccgt acaggatcgt acaaaactgg ttcaggatct gcaaaatgcc
      601 atgcgcaaag ccgttgttgg gccagtggta attgacgggg tccagattga aaatattgat
      661 ttttctgacg cttatgaaaa gtcgattgaa gaccgaatga aagcagaagt cgccattgcc
      721 acgcgtaagc aaaatctgga aacggagaaa atccaggcgc aaatagcggt aactcaggcc
      781 caggcggaag cagacagtaa actagcggca gccaaagcag aagcggaaac tattcgcgta
      841 agaggcgcgg cagaggctga aactattcgt ttaaaaagtg cggctgaagc agaagctatc
      901 cgcctgcgcg gtgaagctct gcgggagaat cccggacttg ttgccctcac cactgctgag
      961 cgttgggacg gtaaactgcc cgataccatg atccccggca gcacagttcc gtttatctct
     1021 actaagtaaa aaaagggccg ctttcgcggc ccttgagatt gatagcaaag acaaaactgc
     1081 ctgatgcgct acgcttatca ggcctacata cttcctgcaa tatgttgaat ttgcaaaatc
     1141 ttgtaggacg gataaggcgt tcacgccgca tccggcataa acaacgcgca ctttgccaac
     1201 aatctgaagg gccgcatttg cggcccttct cacaaagcat cttaccgatt acagcagcgc
     1261 ttttgctttc gcaacaacgt tgtcaacggt gaagccgaac tcttcaaaca gctgctctgc
     1321 cggagcagat tcaccaaagg tggtcatgcc gacgatagca ccgttcaggc caacatactt
     1381 gtaccagtag tcagcaatgc cagcttccac cgcaacacga gcggtaaccg ctttcggcag
     1441 tacggattca cgataagccg cgtcctgctt gtcgaatgcg tcggtagacg gcatggaaac
     1501 cacgcgcgct ttcacgcctt cggcagtcag tttttcgtag gcggcaacag ccagttcaac
     1561 ttcagaaccg gtcgcgatga agatcagttc cggctgacca gcacagtctt tcagcacata
     1621 accaccgcgc gcgatgtttg ccagctgctc ttcagtacgt tcctgctgcg ccaggttctg
     1681 acgggagagg ataagcgcag tcgggccgtc ctgacgctca acgccgtatt tccacgctac
     1741 tgcggattca acctggtcac acggacgcca tgtagacatg ttcggggtca cgcgcagaga
     1801 agcgatctgt tctaccggct ggtgagtcgg gccatcttcg cccagaccga tggagtcgtg
     1861 ggtgtaaacc atcacctgac gctgtttcat cagcgcagcc atacgtacgg cattacgtgc
     1921 gtattccacg aacatcagga aggttgaggt atacggcagg aaaccaccgt gcagggagat
     1981 accgttagca atcgcggtca taccgaactc gcgaacaccg tagtggatgt agttacctgc
     2041 agcatcttcg ttgattgctt tagaaccaga ccacagggtc aggttagacg gagcaaggtc
     2101 agcggagccg ccgaggaatt ccggcaacag cggaccgaac gcttcgatag cattctgaga
     2161 cgctttacgg ctggcgattt tcgccggatt agcctgaagt ttagcaatga actctttcgc
     2221 tttcgcgtcg aagtcagacg gcatttcgcc tttcatacgg cgggtaaatt cagcggcttc
     2281 ctgcggataa gctttcgcgt aagcagcgaa tttctcgttc catgcggact ctttcgcctg
     2341 gcctgcttct ttcgcatccc actgagcata gatttcagac gggatttcga acggcgcgta
     2401 tttccagccc agctgttcgc gggtcagggc aatttcagcg tcgcccagcg gcgcaccgtg
     2461 ggagtcgtgg gtaccggctt tgttcgggga accgaaaccg atgatggttt tgcacatcag
     2521 cagggacggt ttgtcggtca ctgcgcgcgc ttcttctatt gcgcgtttga tggatgccgc
     2581 gtcatgaccg tcgatgtcgc gaataacgtg ccaaccgtaa gcttcgaaac gcattgcggt
     2641 gtcgtcggta aaccagcctt caacgtgacc atcgatagaa ataccgttgt catcgtagaa
     2701 cgcaatcagt ttacccagct tcagcgtacc cgccagagag caaacttcgt gggagatgcc
     2761 ttccatcatg cagccgtcgc ccatgaaggc gtaggtgtag tggtcgacga tgtcgtggcc
     2821 cggacggtta aactgcgccg ccagcgtttt ttctgcaatc gccataccga ctgcgttggc
     2881 aataccctga cccagcggac cggtggtggt ttctacacct gcggtgtaac ccacttccgg
     2941 gtgacccgga gttttagagt gcagctgacg gaagtttttc agttcttcca tcggcagatc
     3001 gtaaccggtg aggtgcagca ggctgtagat cagcatggag ccgtggccgt tggacagcac
     3061 gaagcggtca cggtcagccc aggacggatt ctgcgggttg tgtttcagga aatcacgcca
     3121 caggacttcg gcaatgtcag ccatacccat aggggctccc gggtgaccgg atttggcttt
     3181 ctgtactgcg tccatgctca gcgcacgaat agcattggca agctctttac gtgaggacat
     3241 tttgactcca gatcggatga tgaagggcac acccttaacg acttgacgac agcgcgtttt
     3301 gggctacgcc ggaaaatttg ccaacaattt accgcaagcc gcgcgtcatg tacatggaac
     3361 atccttttgc cgcttcagaa atctctggat catgctcgca tgttgcgcaa tccactcgcc
     3421 cgtccgctgc gcttttcctt atactgagac tgagcgtcga ttcacctgca aacggcgcat
     3481 ttttagaata atcctgacct tgtgcggaag agaaaacatg aaaattcgcg ccttattggt
     3541 agcaatgagc gtggctacgg tgctgaccgg ttgccagaat atggactcca gtggactgct
     3601 ctcatcagga gcggaagctt ttcaggctta cagtttgagt gatgcgcagg tgaaaaaact
     3661 gagcgatcag gcatgccagg agatggacag caaagcgacg attgcgccag ccaatagcga
     3721 atatgctaga cgtctgacga caatttctcg cgcgttaggc gataacatca acggtcagcc
     3781 ggtgaattac aaagtgtata tggcgaagga tgtgaacgcc tttgcaatgg ctaacggctg
     3841 tatccgcgtc tatagcgggc tgatggatat gatgacggat aacgaagtcg aagcggtgct
     3901 cggtcacgaa atggggcacg tggcattagg ccatgtgaag aaaggaatgc aggtagcact
     3961 tggtacaaac gcaattcgcg ttgcggcggc ctctgcaggc ggaattgtcg gcagtttgtc
     4021 acaatcacaa ctgggagacc tgggtgaaaa gctagtcaat tcgcaattta cccaacgtca
     4081 ggaatcagag gccgatgatt actcctacga tttgctgcgc caacgcggta tcagcccggc
     4141 aggtcttgcc accagctttg aaaaactggc gaaactggaa gaaggccgcc aaagctcaat
     4201 gtttgacgac catcctgcat ccgccgaacg cgcccagcat attcgcgatc gtatgagcgc
     4261 ggatggagtt aagtaaagcc aggtagtgcc gaacgtaggt cggataaggc gttcacgccg
     4321 catccgacat taatgacaca tcactcgtgc gcctcttatc tggcgcttac tcgccctttt
     4381 tcgccgcctg gatatacagc atctccagcg ccagcgtcgc cgcagccaga gcggtgattt
     4441 ccgactgatc gtatgccgga gccacctcca ctacgtccat cccaacgatg ttgagatctt
     4501 tcaggccgcg taccagttta atggcgcgat cggaggtcag gccgccaatc accggcgtac
     4561 cagttcccgg cgcgaaagca ggatccaggc agtcgatatc aaaggtcagg taaaccggca
     4621 tatcacctac aatctgtttc acctgagcga taacgtcatc cacgctgcga tcgttcacct
     4681 ggcaggcgtc cagcacggta aagccgttgt ctttatcaaa ctcggtacga ataccaatct
     4741 gcacggaatg attcgggtcg atcagacctt ctttcggcgc ggtatagaac atggtgccgt
     4801 ggtcaaattc acaaccgtta gcgtaggtat cggtgtgggc gtcaaagtgt accagcgcca
     4861 ttttaccgaa atgcttcgca tgagcacgca gcagcggcag cgtaacaaag tggtcaccac
     4921 cgaaggagag catacgctta ccggcagcca gcagcttctc ggcgtgcgcc tgcagcttct
     4981 cgctcatctc acgggcatct ccgaaggcat ataccagatc gccgcagtcc acgacgttca
     5041 ggcgctcacg catgtcgaaa ttccacggga agcgattgtg ttcccaggcc agattcgtcg
     5101 aaacctgacg gatcgctgcc ggaccgtggc gaccacccgc acgaccagaa gtggccatat
     5161 cgaacggcac gccagtaatc acccagtcag catcgctgtc atatggctgg aagttcatcg
     5221 gcaggcgtaa aaaaccaaag gcgttggaaa ccagtgagtt atcgtattga tgacctaagg
     5281 tgctcatgga tggactccta ttacaaagtc gatataaaaa aacccctccg cgtcgttagg
     5341 cccgacgagg aagggttgga tttgcgaaac acaagtttgt gctaattatc gccctaaatg
     5401 ggcaaggcat caagcaaggt tactatttcc gacacggtgg cggcgcatag ccattgttat
     5461 ctgccggaaa cgcttgtcca gagcacggcg gcagcgcgtt ttgcgccggg ccttgtaatt
     5521 caaaggcggc aaagccgatt acaccggtat tacgcacatc gccttgcgcc gaattggctg
     5581 cgtaggaatc agcaacgttg gcgaactgaa aagccgcttc ggtatgttta tctttgcgga
     5641 atcctttaat cgctaacgaa tctccggcat tcacaatata accgttgtta ttcagcgaac
     5701 cttgtttacc attcagaaca tccaggccat caaccgtagc aacgatctcg tagttggtgt
     5761 tacgactgta attacggaca tataattgat aattcattcc gactctggca tggacaatat
     5821 agcgtggttc attattggat gaatcataaa cccgcgtaat tggtagagat ttaaaattac
     5881 catcgcgaat agcatattcc agttcgttaa tacgaatgct ataaacgcgg tcgtagccgg
     5941 aaggaacatt agtcgaataa ctaataacga tcatctctgc cggatcgcgc caggctcgtt
     6001 cagcagatac gccctgaacc gttgagtgca catccccccc cccaggcggt gccgacatta
     6061 cccgatgcac tgctattata ctgcgcatta ctggaacaac cggccagcag cacggctaat
     6121 gctatcaacg cgctacgtac tttccatttt ttcataaccg ttccctctgc atttaat
//
LOCUS       DADSYM010000195         6027 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2415
            SAMN08039579-rid11723173.denovo.197, whole genome shotgun sequence.
ACCESSION   DADSYM010000195 DADSYM010000000
VERSION     DADSYM010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039579
            Sequence Read Archive: SRR6362533
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6027)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6027)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980162.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 03:39:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,484
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 4,347
            CDSs (with protein)               :: 4,347
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 43 of 85
            Pseudo Genes (incomplete)         :: 37 of 85
            Pseudo Genes (internal stop)      :: 19 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6027
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039579-rid11723173.denovo.197"
                     /strain="LGE2415"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8302 N 95.9628 W"
                     /collection_date="2015-08-24"
     gene            250..1470
                     /locus_tag="J1Y83_004027"
     CDS             250..1470
                     /locus_tag="J1Y83_004027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001744284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HBA7632120.1"
                     /translation="MSIKSQAYLFKRKDGIWMFQIFVPKYLRHLYPYKLWRKSTSTRD
                     LNEARRFRNHLLLEWEELKRKHNPDNPERRLQQAIESLHSEARANKNTQRAVKRGYAP
                     PSLCLIRDEYAECYRERRSYSTLQKSARAVELFLQSIQKDDIHIEKIGRRLVTVFLDD
                     QKQRGAADQSRQNWLTCLGSLYEFAKRRYDGIPDSNPFHGHNLEARRTIESYQPFTPE
                     QMQKLLDAAEPEIRNIILMGLFSGARLDELASLKKSEIVTVEGIRCFYISKSKTKAGI
                     RHVPIHSRLSIIVDEYLNHNYGEYLFPHSNKVKRTDGKKGPFYSQAFSRLRRSVLPIA
                     TNRQCFHSLRGMFITCLDRAGVPEQRIGAITGHTEQIAKTEAFRTYSQGASIPELAEY
                     VELVKYELNIDFKA"
     gene            complement(1578..3419)
                     /locus_tag="J1Y83_004028"
     CDS             complement(1578..3419)
                     /locus_tag="J1Y83_004028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000422416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA7632121.1"
                     /translation="MELNISTLTNRVVFKVDFASLQKARDAGKKFQKEMENLTNPTLK
                     GKKAKRAIDDANDLVKARKAAQAKADAKASDKQAQTDKKNADKVAKAEAKAKAKAAIA
                     ADKQIRAKATIAEKAEIKRQQAFRQLEGIKKGRMGDGMDSAQHYKALQYIKAQTAAYE
                     KGAISQAKMNHLIRDRITLERRAAAVKARQVAADGGKYPHTAKNIKGGHMGAGIGAMV
                     GGAALTAATLGSAAIFNTVTDQASKITDTNRKAKAVNVNTNALLALNQYGLEHGVDSS
                     VDQRQDNLKDVQERLGETVGNTKWDAKKGRWTGGNGAVDNILNKYGWSPEQVKGFQTD
                     PLGFLGAVVKEGERRGLSDQEISHILEDLGNDLAYFRGAFSRNGEELNKTIVRMQQAN
                     ATYTQADLDAAERLQAVQANMNMAWEGSKKAIMTGFGEEFDAQAGEEWAQMLKDLNPL
                     FKELGSSTAEMMSAFAGAVSWLTAAYQNINPENKHENLDSSGMYYKDSFVGGMVDWGR
                     CALGLSPIPGTEDKPTTGGTAIQNYSSPSDNVQSLKQNVSRDLNTTPSMTYAPNIVVN
                     VPESKVDVNIAPDGSQLGSYLDYKVGSIQQDFERSFTLAALSGQAPS"
     gene            complement(3438..3629)
                     /locus_tag="J1Y83_004029"
     CDS             complement(3438..3629)
                     /locus_tag="J1Y83_004029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA7632122.1"
                     /translation="MNTQNNNPVEILKAVSTLPEDILNISQVCKFLGISNTAVRQRIF
                     ATGCSAMEAVQYYVLKQNK"
     gene            complement(3767..4795)
                     /locus_tag="J1Y83_004030"
     CDS             complement(3767..4795)
                     /locus_tag="J1Y83_004030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000583442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage capsid protein"
                     /protein_id="HBA7632123.1"
                     /translation="MIIGNESVDLAPLFSLHDSRNFLLTTLDFTDPVGVSSHKVAVSQ
                     LVETNESLFNKETSRFSSEHNVTKRENGKEWLVEIPYFLREDMIKPQDVQGKRKPGAT
                     VEETLTDIYAEYVAKHHIAFQRTKESVLAASLFSGKTYTPKTDDVLIQWGDLFSVVPM
                     TATIDASSTTPSMVFQQFDQMHTDVITKAQSQAAAVERIVVFCKPAAFNAIRYSAGMV
                     NAFQYASPTDDANIVYSVRSLLPGVTTFTIPGTNIDIIKVTDPLLLAHMTSDAVAVPK
                     FAKGSNVYQNIFGAPSSTFDLLNSGAAEYYSYAFSPARNDAVNVVTENSQMVVNHGVG
                     FTVQITVQ"
     gene            complement(4814..5185)
                     /locus_tag="J1Y83_004031"
     CDS             complement(4814..5185)
                     /locus_tag="J1Y83_004031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000974765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA7632124.1"
                     /translation="MAIINMSDLFPVRKSFTDIVSYATDEFGCVQSFTVDLAVDVECG
                     DLITSDGAEYVSGGDVLVVVSDAVSAGDAKTVNVLRSNAGLVCIKSDALNVEGAIKEA
                     AIAALAAKGFQMAGFNTVFTS"
     gene            complement(5188..5403)
                     /locus_tag="J1Y83_004032"
     CDS             complement(5188..5403)
                     /locus_tag="J1Y83_004032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001180367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA7632125.1"
                     /translation="MQNLILKNGNEELIITDETFNATAYMLGITPTELKKHCDKLFKS
                     ADEQKPTMQDLIKAKAEQIKQSKKGNY"
BASE COUNT         1691 a         1310 c         1296 g         1730 t
ORIGIN      
        1 gattgcattg cgtaaacgct ttttatttac aaaaaaatgg ggaagtatta cggcgattgc
       61 gaaaagtctg aagcgcttga attaagataa tatagaaaat cagatacaac aaaaatggca
      121 ggtagatgat gttaagataa attggagcgg gcgaagggaa tcgaggaaac ccataataac
      181 ctgctgtaaa ataaagattt tgtgatcaat cataggtaac tgattacgtg ataatagagg
      241 atcattatcg tgtcgatcaa gagccaagca tacttattta aaagaaaaga tgggatctgg
      301 atgtttcaga tttttgttcc taaatactta cgtcatcttt acccttataa gttgtggaga
      361 aaaagcacat ctacacgaga tctgaatgag gcgagaagat tcagaaacca tctattatta
      421 gaatgggagg aactaaagcg gaaacataat cctgataacc ccgaaaggag gttacaacaa
      481 gctattgaat ccttacattc agaggcaagg gccaataaaa acacgcaaag agccgtaaaa
      541 cgcggttatg caccgccttc cctgtgccta atacgtgacg aatacgccga atgctacaga
      601 gagcgtcgtt catactcaac actacagaaa tcagcaaggg ccgttgagct atttttacaa
      661 agcattcaga aagatgatat tcatattgag aaaataggca gacggcttgt aacagtattc
      721 ttagatgacc agaagcaacg aggagcagcc gatcaatctc gtcaaaactg gcttacatgc
      781 ttaggatcgc tctatgaatt tgctaaacgc cgttatgacg gaataccaga cagtaaccct
      841 tttcacggtc ataacctcga agcacgtcgc acaatcgaga gttatcaacc gtttacacct
      901 gaacaaatgc aaaagctact tgatgcagcg gagccggaga tcagaaatat tatcttaatg
      961 ggcttgtttt caggggccag attagacgaa ctggcaagcc tgaaaaagtc agagattgta
     1021 actgtggaag gaatacgctg tttttatatc agcaagagta agactaaggc agggatcaga
     1081 cacgttccga tacattcgag actatcgatt attgtcgatg aatatttaaa tcataattat
     1141 ggtgaatatt tgttccctca ctcaaataaa gtaaaaagaa ccgacggtaa gaaaggcccg
     1201 ttctattctc aagcgttctc acgtttacgt cgtagcgtct tgccaatcgc tacaaataga
     1261 caatgctttc acagcttgcg cgggatgttt atcacttgtc ttgatcgtgc aggagtgcct
     1321 gaacagcgga tcggagccat taccggacat acagagcaga tagctaaaac agaagccttt
     1381 cgcacctact cacaaggggc aagcattcca gaattagctg aatatgtaga attagtaaaa
     1441 tatgaattaa atattgattt taaggcgtaa aacaacgcca taaacgtaat aaaaaggctt
     1501 ccttgtaatg ggagcctctt atatgcttaa ttccttacaa gagtgtttta gattgtgatt
     1561 aaacattctc tttatcatta acttggtgct tgcccagaca gagccgccaa cgtgaaacta
     1621 cgttcaaaat cttgttggat acttcccact ttgtaatcga ggtatgatcc aagctgacta
     1681 ccatcagggg cgatattcac atccaccttg ctttccggca cgtttacgac gatgttcggt
     1741 gcatacgtca tagatggggt agtgttcaga tcacggctaa cgttctgttt cagggattgc
     1801 acgttatcgg atgggcttga atagttctga attgcagtgc cgccagtcgt tggcttgtct
     1861 tccgtgccag ggataggcga taagcctaaa gcacaacgac cccaatcaac cataccacct
     1921 acaaacgagt ccttataata cataccggac gaatcaaggt tttcatgttt gttctctgga
     1981 tttatattct ggtatgcagc agtaagccac gatacagcac cagcaaaggc actcatcatt
     2041 tcagccgttg agcttccaag ttctttaaac agaggattga gatctttcaa catttgcgcc
     2101 cactcttcac cagcttgagc gtcgaactct tcaccgaatc ccgtcatgat agctttctta
     2161 ctaccttccc aggccatatt catattagcc tgtacagctt gcagacgttc agcagcgtca
     2221 agatccgctt gcgtataggt ggcgttggct tgttgcatcc tgacgatggt tttatttagc
     2281 tcttcgccgt tacggctaaa cgcgccgcga aaataggcaa gatcgtttcc aaggtcttct
     2341 aaaatatgag aaatttcttg atcagagagt ccacggcgtt caccttcttt cactacagcg
     2401 cccaggaaac ctaatggatc agtctggaaa cctttaacct gttcaggact ccaaccatat
     2461 ttattaagga tgttatcgac ggcaccgtta ccgccagtcc agcgaccttt tttagcgtcc
     2521 catttggtat tacctaccgt ttcgcctaaa cgctcctgaa cgtctttaag gttatcttga
     2581 cgttgatcga cggaactatc aacaccgtgt tctaagccgt actgattaag tgctaacagg
     2641 gcgttagtgt ttacgttgac agcctttgcc ttgcggttag tgtctgtgat cttgcttgcc
     2701 tgatcggtta cggtattgaa tatcgcagca ctgccgagag tagcagcagt gagagcggca
     2761 ccgcctacca ttgcaccgat accagcgccc atatgaccgc ctttgatgtt cttggctgta
     2821 tgtggatact tgccaccatc agccgccacc tggcgagctt ttacagcagc cgcacggcgt
     2881 tctaacgtga tgcgatcgcg tattaaatgg ttcattttcg cctgactgat agcgcctttc
     2941 tcatacgctg ccgtttgtgc tttgatgtat tgcaatgctt tgtagtgctg ggcggagtcc
     3001 ataccgtcac ccatacgccc cttcttgatg ccttcaagct gtctaaaagc ttgctggcgt
     3061 ttaatctctg ctttctctgc tatcgttgct ttagcgcgga tctgtttatc ggcagctatc
     3121 gcggctttag ccttcgcttt agcttctgcc tttgccacct tatcagcatt cttcttatct
     3181 gtttgagctt gcttgtctga cgctttagca tcagctttag cctgtgcagc tttacgggcc
     3241 tttaccagat cgttagcatc gtctatagct cgtttagcct tcttcccttt caatgttgga
     3301 ttggttaggt tttccatttc tttctgaaat ttcttacctg catcgcgtgc tttctgtaat
     3361 gatgcaaaat caactttaaa aacgacgcgg ttcgttaagg ttgagatgtt caactccata
     3421 atattttcct ttatctctta tttattctgt tttaatacgt aatattgcac tgcttccata
     3481 gcggagcagc ccgtagcaaa aattctttga cgtacagcgg tattgctgat ccctaaaaac
     3541 ttacatactt gtgagatatt tagaatatct tccggtaagg tggaaacagc tttcaagatt
     3601 tcaactggat tgttattttg tgtgttcatg gtttttctct cacaatattt aataaacggt
     3661 agaaaacaaa aagggcaaag agatcagagc atattttgcg tatatggtta atcccttcgc
     3721 cctattgccc ccgattattt aaagacggcg gggaacgtct gatatcttac tgaacggtga
     3781 tctgtacggt gaagcctacg ccgtggttta caaccatctg gctattctcg gtaactacgt
     3841 ttacagcatc gttacgtgct ggactaaacg catagctgta atattcagcc gcgccagaat
     3901 tcagcaagtc aaacgttgaa gaaggagcgc cgaaaatgtt ctgatagacg ttagagcctt
     3961 tagcgaactt cggaacagca accgcatcag aagtcatatg agccagcagc agcggatcag
     4021 taactttgat gatatcgatg ttagtccctg gaatagtgaa ggtagtcaca cctggcagca
     4081 gggagcgcac actataaacg atgtttgcgt cgtcggtagg gcttgcatac tggaaagcgt
     4141 taaccatgcc agcactataa cggatagcgt tgaaagcagc aggtttacag aatactacga
     4201 tacgttctac agccgctgcc tgactttggg ccttagtgat aacatctgtg tgcatctgat
     4261 cgaattgctg gaacaccata gaaggcgtgg tgcttgatgc gtcgatggtt gcagtcatcg
     4321 gaacaacact aaacagatcg ccccactgga tcagcacatc atcagtttta ggggtgtaag
     4381 ttttaccaga gaacagggaa gcagccagta cgctttcttt agtacgctgg aaagcaatgt
     4441 ggtgcttagc aacatattcc gcgtagatat ctgtcagagt ctcttcaacg gtagcgcctg
     4501 gtttacgttt gccttgaacg tcttgcggct taatcatatc ttcacgcagg aagtaaggga
     4561 tctctaccag ccattcttta ccgttctcac gcttggtcac gttatgttca gaagagaaac
     4621 gagaggtttc tttgttgaac agagattcgt tagtttccac cagttggcta acagccactt
     4681 tatgagaact cacacctacc ggatcagtga agtccagagt agttaacagg aagttgcgag
     4741 agtcgtgcag actaaacagc ggagccagat cgacggattc attaccaata atcattatgt
     4801 ttattccttt atattaagat gtgaatacag tgttaaaacc agccatttgg aagcctttag
     4861 cagccagagc agcaatagcg gcttctttaa tagcgccttc aacattcaga gcatcagact
     4921 tgatgcaaac cagtccggca ttgctacgca gcacgttaac ggttttagcg tcgccagcgg
     4981 ataccgcatc agacacaacc acaagaacat cgccgccgct tacatattca gcgccgtcac
     5041 tggtaatcag atcgccgcat tcaacatcaa ccgccagatc aacggtaaaa gattgcacac
     5101 aaccgaactc atcagttgca tatgaaacga tatcggtaaa tgatttgcga actggaaaca
     5161 gatcgctcat attaataata gccataatta atagtttcct tttttggatt gtttgatttg
     5221 ttctgctttt gctttaatca gatcctgcat agtaggtttc tgttcatcag cagacttaaa
     5281 aagcttatca caatgttttt tcaattctgt tggagtgatc cccaacatat aagccgtagc
     5341 attgaatgtt tcatcagtga taatcaattc ttcgttacca ttctttagaa tgagattttg
     5401 cattgttgct ttacctttta ttcctaacat tcttgtttag tgctgataaa ataaaaccgg
     5461 aaggggaaag cttccggctt tatgaataca acattgaagg atgttataga gtagtgttac
     5521 taaatcgcag ttatatgcac ttaaagaggt aggacatata agaataagat ttcttattgt
     5581 tataagccct tgaggggtga ataacaggct tacagaggag taggcgctta ggaggtcgcc
     5641 cccttggaaa ggaatcattg taaacctgtt attcgtgcga tggctaatcg ctgtctttct
     5701 caatgtggat gcatcgatca gactattcag gaatcaccta ttaactttca aaccttttgg
     5761 attctcattt taataggagg cggtgccgag atgatttcgg ggatcatcca cgctttcgcg
     5821 ttctaagatt gacaccttaa taaacaccaa caaaaacata ttcagctcat tgttgtcaag
     5881 aaacaatata caccgttatt tgtttatacg gggtttatag aacactgaaa aacagtgctt
     5941 ttagaaagtg tttcgtgttt catgcttcat agttgattaa aagatgaggg gccaagtcca
     6001 tagcccctgc attctcaaat acacaat
//
LOCUS       DADSYM010000196         5914 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2415
            SAMN08039579-rid11723173.denovo.198, whole genome shotgun sequence.
ACCESSION   DADSYM010000196 DADSYM010000000
VERSION     DADSYM010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039579
            Sequence Read Archive: SRR6362533
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5914)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5914)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980162.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 03:39:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,484
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 4,347
            CDSs (with protein)               :: 4,347
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 43 of 85
            Pseudo Genes (incomplete)         :: 37 of 85
            Pseudo Genes (internal stop)      :: 19 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5914
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039579-rid11723173.denovo.198"
                     /strain="LGE2415"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8302 N 95.9628 W"
                     /collection_date="2015-08-24"
     gene            complement(150..896)
                     /gene="dsbG"
                     /locus_tag="J1Y83_004033"
     CDS             complement(150..896)
                     /gene="dsbG"
                     /locus_tag="J1Y83_004033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415137.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbG"
                     /protein_id="HBA7632126.1"
                     /translation="MLKKILLLALLPAIAFAEELPAPVKAIEKQGITIIKTFDAPGGM
                     KGYLGKYQDMGVTIYLTPDGKHAISGYMYNEKGENLSNTLIEKEIYAPAGREMWQRME
                     QSHWLLDGKKDAPVIVYVFADPFCPYCKQFWQQARPWVDSGKVQLRTLLVGVIKPESP
                     ATAAAILASKDPAKTWQQYEASGGKLKLSVAANVSTEQMKVLSDNEKLMDDLGANVTP
                     AIYYMSKENTLQQAVGLPDQKTLNIIMGNK"
     gene            1268..1831
                     /gene="ahpC"
                     /locus_tag="J1Y83_004034"
     CDS             1268..1831
                     /gene="ahpC"
                     /locus_tag="J1Y83_004034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003858764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit C"
                     /protein_id="HBA7632127.1"
                     /translation="MSLINTKIKPFKNQAFKNGEFIEITEKDTEGRWSVFFFYPADFT
                     FVCPTELGDVADHYEELQKLGVDVYAVSTDTHFTHKAWHSSSETIAKIKYAMIGDPTG
                     ALTRNFDNMREDEGLADRATFVVDPQGIIQAIEVTAEGIGRDASDLLRKIKAAQYVAS
                     HPGEVCPAKWKEGEATLAPSLDLVGKI"
     gene            2002..3567
                     /gene="ahpF"
                     /locus_tag="J1Y83_004035"
     CDS             2002..3567
                     /gene="ahpF"
                     /locus_tag="J1Y83_004035"
                     /EC_number="1.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415139.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HBA7632128.1"
                     /translation="MLDTNMKTQLKAYLEKLTKPVELIATLDDSAKSAEIKELLAEIA
                     ELSDKVTFKEDNSLPVRKPSFLITNPGSNQGPRFAGSPLGHEFTSLVLALLWTGGHPS
                     KEAQSLLEQIRHIDGDFEFETYYSLSCHNCPDVVQALNLMSVLNPRIKHTAIDGGTFQ
                     NEITDRNVMGVPAVFVNGKEFGQGRMTLTEIVAKIDTGAEKRAAEELNKRDAYDVLIV
                     GSGPAGAAAAIYSARKGIRTGLMGERFGGQILDTVDIENYISVPKTEGQKLAGALKVH
                     VDEYDVDVIDSQSASKLIPAAVEGGLHQIETASGAVLKARSIIVATGAKWRNMNVPGE
                     DQYRTKGVTYCPHCDGPLFKGKRVAVIGGGNSGVEAAIDLAGIVEHVTLLEFAPEMKA
                     DQVLQDKLRSLKNVDIILNAQTTEVKGDGSKVVGLEYRDRVSGDIHNIELAGIFVQIG
                     LLPNTNWLEGAVERNRMGEIIIDAKCETNVKGVFAAGDCTTVPYKQIIIATGEGAKAS
                     LSAFDYLIRTKTA"
     gene            complement(3688..4116)
                     /gene="uspG"
                     /locus_tag="J1Y83_004036"
     CDS             complement(3688..4116)
                     /gene="uspG"
                     /locus_tag="J1Y83_004036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspG"
                     /protein_id="HBA7632129.1"
                     /translation="MYKTIIMPVDVFEMELSDKAVRHAEFLAQDDGVIHLLHVLPGSA
                     SLSLHRFAADVRRFEEHLQHEAEERLQTMVSHFTIDPSRIKQHVRFGSVRDEVNELAK
                     ELDADVVVIGSRNPSISTHLLGSNASSVIRHANLPVLVVR"
     gene            complement(4472..4882)
                     /gene="rnk"
                     /locus_tag="J1Y83_004037"
     CDS             complement(4472..4882)
                     /gene="rnk"
                     /locus_tag="J1Y83_004037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside diphosphate kinase regulator"
                     /protein_id="HBA7632130.1"
                     /translation="MSRPTIIINDLDAERIDILLEQPAYAGLPIADALNAELDRAQMC
                     SPEEMPHDVVTMNSRVKFRNLSDGEVRVRTLVYPAKMTDSNTQLSVMAPVGAALLGLR
                     VGDSIHWELPGGVATHLEVLELEYQPEAAGDYLL"
     gene            complement(5112..>5914)
                     /gene="rna"
                     /locus_tag="J1Y83_004038"
     CDS             complement(5112..>5914)
                     /gene="rna"
                     /locus_tag="J1Y83_004038"
                     /EC_number="4.6.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ribonuclease I"
                     /protein_id="HBA7632131.1"
                     /translation="AFWRNAALLAVSLLPFSSANAVALQAKQYGDFDRYVLALSWQTG
                     FCQSQHDRNRNERDECRLQTETTNKADFLTVHGLWPGLPKSVAARGVDERRWMRFGCA
                     TRPIPNLPEARASRMCSSPETGLSLETAAKLSEVMPGAGGRSCLERYEYAKHGACFGF
                     DPDAYFGTMVRLNQEIKESEAGKFLADNYGNTVSRRDFDAAFAKSWGKENVKAVKLTC
                     QGNPAYLTEIQISIKADTINAPLSANSFLPQPHPGNCGKTFVIDKAGY"
BASE COUNT         1508 a         1399 c         1501 g         1506 t
ORIGIN      
        1 gttgtattta tatgtattat gtttttctgc ttaactttgg attaagttta tctaaaatag
       61 catagctgtc aggtgggtta cgagcaattc atgcgtttta aagatggttg aaaagcagag
      121 agagatttca accatcgatg aattatattc tatttattcc ccataatgat attaagtgtt
      181 ttctgatcgg gtaatccgac ggcttgttgc agggtatttt ccttactcat gtaatagata
      241 gctggtgtga catttgcccc cagatcgtcc atgagtttct cgttgtcgct taacactttc
      301 atttgctctg tacttacgtt tgcagccacg cttagcttaa gcttgccacc agaggcttca
      361 tattgttgcc aggtttttgc gggatctttg gaggcaagaa ttgccgctgc tgtcgccggg
      421 ctttctggct tgataacccc aaccaacaat gttctcaatt gcactttgcc agaatctacc
      481 cacggacgag cctgctgcca gaactgttta caatatgggc agaacgggtc ggcgaagacg
      541 tagacaatga ccggcgcgtc ttttttaccg tcgaggagcc agtgggattg ttccatccgt
      601 tgccacattt cacgtccggc gggtgcgtaa atttcttttt cgataagcgt gttactcagg
      661 ttttcacctt tttcgttgta catgtaacca gagatagcgt gcttaccatc tggagtcagg
      721 tagatggtga cgcccatatc ctgatactta ccgagataac ctttcattcc tccaggggca
      781 tcgaacgttt tgatgattgt aataccctgt ttttcaatcg cttttactgg ggcaggaagt
      841 tcctctgcga aggcgattgc aggaagcagg gccagtaaaa gtatcttttt taacattaat
      901 ttgtcctttt cagtcagtgc aaaagtcgag taaaaggcat aacctatcac tgtcataggt
      961 aagagcttag atcaggtgat tgccctttat ttatgagggt gttgtaatcc atgtcgttgt
     1021 tgcatttgta agggcaacac ctcagcctgc aggcaggcgc tgaagatacc aaagggtagt
     1081 tcagattaca cggtcacctg gaaagggggc cattttactt tttatcgccg ctggcggtgc
     1141 aaagttcaca aagttgtctt acgaaggttg taaggtaaaa cttatcgatt tgataatgga
     1201 aacgcattag ccgaatcggc aaaaattggt taccttacat ctcatcgaaa acacggagga
     1261 agtatagatg tccttgatta acaccaaaat taaacctttt aaaaaccagg cattcaaaaa
     1321 cggcgaattc atcgaaatca ccgaaaaaga taccgaaggc cgctggagcg tcttcttctt
     1381 ttacccggct gactttactt tcgtatgccc gaccgaactg ggtgacgttg ctgaccatta
     1441 cgaagaattg cagaaactgg gcgtagacgt atacgcagta tctaccgata ctcacttcac
     1501 ccacaaagca tggcacagca gctctgaaac catcgctaaa atcaaatatg cgatgatcgg
     1561 cgacccgact ggcgccctga cccgtaactt cgacaacatg cgtgaagatg aaggtctggc
     1621 tgaccgtgcg accttcgttg ttgacccgca gggtatcatc caggcaatcg aagttaccgc
     1681 tgaaggcatt ggccgtgacg cgtctgacct gctgcgtaaa atcaaagcag cacagtacgt
     1741 agcttctcac ccaggtgaag tttgcccggc taaatggaaa gaaggtgaag caactctggc
     1801 tccgtctctg gacctggttg gtaaaatcta aatttccttt gtctttcgcg ctactaactc
     1861 cggcgcgaga tacttcggaa attcacctaa ttcttcgggt gctgcggcac ccgattttct
     1921 tccccgcacc atgatgcaag ctgcatccag ctagccgcag aggccgcttg catgatgatg
     1981 tttaaagccc aggagataaa catgctcgac acaaatatga aaactcaact caaggcttac
     2041 cttgagaaat tgaccaagcc tgttgagtta attgccacgc tggatgacag cgctaaatcg
     2101 gcagaaatca aggaactgtt ggctgaaatc gcagaactgt cagacaaagt cacctttaaa
     2161 gaagataaca gcttgccagt gcgtaagccg tctttcctga tcaccaaccc aggttccaac
     2221 cagggaccac gttttgcagg ctccccgctg ggccacgagt tcacctcgct ggtactggcg
     2281 ttgttgtgga ctggtggtca tccgtcgaaa gaagcgcagt ctctgctgga gcagattcgc
     2341 catattgacg gtgattttga attcgaaacc tattactcgc tctcttgcca caactgccct
     2401 gacgtggtgc aggcgctgaa cctgatgagc gtactgaacc cgcgcatcaa gcacactgca
     2461 attgacggcg gcaccttcca gaacgaaatc accgatcgca acgtgatggg cgttccggca
     2521 gtgttcgtaa acgggaaaga gtttggtcag ggccgcatga cgttgactga aatcgttgcc
     2581 aaaattgata ctggtgcgga aaaacgtgcg gcagaagagc tgaacaagcg tgatgcttat
     2641 gacgtattaa tcgttggttc cggcccggcg ggtgcagcgg cagcaattta ctccgcacgt
     2701 aaaggcatcc gtaccggtct gatgggcgaa cgttttggtg gtcagatcct cgataccgtt
     2761 gatatcgaaa actacatttc tgtaccgaag accgaaggcc agaaactggc aggtgcgctg
     2821 aaagttcatg ttgatgaata cgacgttgat gtgatcgaca gccagagtgc gagcaaactg
     2881 atcccggcag cggttgaagg cggcctgcat cagattgaaa cagcttctgg cgcggtactg
     2941 aaagcacgca gcattatcgt ggcgactggt gcaaaatggc gcaacatgaa cgttcctggc
     3001 gaagatcagt atcgcaccaa aggcgtgacc tactgcccgc actgcgacgg cccgctgttt
     3061 aaaggcaaac gcgtagcggt tatcggcggc ggtaactccg gcgtggaagc ggcaattgac
     3121 ctggcgggta tcgttgagca cgtaacgctg ctggaatttg cgccagaaat gaaagccgac
     3181 caggttctgc aggacaaact gcgcagcctg aaaaacgtcg acattattct gaatgcgcaa
     3241 accacggaag tgaaaggcga cggtagcaaa gtcgtaggtc tggaatatcg cgatcgtgtc
     3301 agcggcgata ttcacaacat cgaactggcc ggtattttcg tccagattgg tctgctgccg
     3361 aacaccaact ggctggaagg cgcagtcgaa cgtaatcgca tgggcgagat tatcattgat
     3421 gcgaaatgcg aaaccaacgt caaaggcgtg ttcgcagcgg gtgactgtac gacggttccg
     3481 tacaagcaga tcatcatcgc cactggcgaa ggtgccaaag cctctctgag tgcttttgac
     3541 tacctgattc gcaccaaaac tgcataagaa gaagtaagat tcacctgtaa ttgcttagcc
     3601 gccggggtca aacctggcgg cttttttatt gcattaaaaa gccctgcagg gatggctccg
     3661 ggcagggcgg gagacttatt ctggcaatta acgcacaacc agcaccggca aattggcgtg
     3721 gcggattacg ctcgaggcgt tagaacccaa cagatgagtt gaaattgatg ggttacgcga
     3781 accaatcact actacatctg catcaagttc tttcgctaac tcgttaactt catcccgcac
     3841 actaccaaaa cggacatgtt gcttaatgcg ggaaggatcg atggtgaagt ggctgaccat
     3901 cgtttgtaaa cgttcttctg cttcatgttg cagatgctct tcaaaacgac gtacatcagc
     3961 ggcaaaacgg tgcaggctca ggctggctga ccccggtagt acgtgaagta gatgaataac
     4021 tccgtcatcc tgggcgagga attcagcgtg gcgaaccgct ttgtcgctca attccatttc
     4081 gaaaacatcc actggcatga tgattgtttt gtacataagc attctctcct tgtttttgca
     4141 tgtatacata acagcacatt attggttatg aatctatgat ttgcgaggct gatttgatct
     4201 gaatttgcta ttgagctgga aaataaataa aaccaggcgc taccagcgtt gttcaaggta
     4261 tagcactgca gcaacaataa ttgcggctat gaatatataa aatgcagtga taccgagagc
     4321 ttcgctcata tgaggttccg cagatgaata accagttcag attagaatgc tgcgcttaag
     4381 agcaaaatga taaatctggt tgatatttga gcggaaagag gatgcgagtg catcctctgg
     4441 gcaaagcgag ttgtcgcttg tgcagatggg attaaagcag gtagtcgcca gcagcttctg
     4501 gctggtattc gagttccagc acttcaaggt gggttgcaac gccgcccgga agttcccagt
     4561 gaatggaatc gccaacgcgc agccccagca gtgcggcacc taccggagcc ataacggaaa
     4621 gctgagtatt gctgtcggtc atttttgccg gatacaccag cgtgcgcaca cgcacttcgc
     4681 catcgctaag attgcggaat ttaacccggc tgttcattgt caccacgtcg tgtggcatct
     4741 cttctggcga acacatttgg gcgcgatcca actctgcgtt taacgcgtcg gcgattggca
     4801 aaccagcata ggcgggttgc tccagcagaa tatcgatgcg ttcggcatcc aggtcgttaa
     4861 tgatgatagt tggtctggac atttttactc catgtcgtcg gtactgcgag tgtcgcagat
     4921 aaacataccc aaaagaaaac cctcaccgtc aggcggcgag ggtttaactc acatgatgat
     4981 actgactgtt gctcactctt tgaagtgatt tgcgtcacat tcagggaatt cctcaatgta
     5041 gcgcattatg tataaatctt aatcgtcatg gtttatggaa gacgaattag cgtgttttgt
     5101 aaatcagagg attaataacc tgctttatca atcacaaagg ttttgccaca gttgcctggg
     5161 tgtggttgtg gcaagaatga gttggcagaa agcggagcgt tgatggtgtc agctttgatc
     5221 gagatctgaa tttcagttaa atacgcaggg ttaccctggc acgttagctt aactgctttc
     5281 acgttctctt ttccccagct tttggcaaag gcggcatcaa agtcacggcg gctcactgtg
     5341 ttaccgtagt tatccgcaag gaattttccg gcttcgctct ctttaatttc ttgattcagg
     5401 cgtaccatcg tgccgaagta tgcgtctgga tcaaaaccaa agcaggcacc gtgtttggca
     5461 tattcgtagc gttccaggca ggaacgtccg ccagctcctg gcatgacttc acttagttta
     5521 gctgccgttt ccagtgataa cccagtttcc ggcgatgaac acatccggct ggcgcgtgct
     5581 tctggcagat tcgggattgg gcgagtagcg caaccgaagc gcatccagcg gcgttcatca
     5641 acaccacggg cagcaaccga tttaggcaat cctggccaca gaccatgtac ggtcaaaaaa
     5701 tcagctttgt tggtcgtttc ggtttgcagg cgacattcat ctcgttcgtt acgatttcga
     5761 tcatgttgac tctggcaaaa tccggtttgc caggagaggg ccaggacata gcgatcaaaa
     5821 tcgccatact gttttgcctg caacgccacg gcgttggcag aagagaaggg aagcagagaa
     5881 accgcgagca acgcggcgtt acgccagaat gctt
//
LOCUS       DADSYM010000197         5689 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2415
            SAMN08039579-rid11723173.denovo.199, whole genome shotgun sequence.
ACCESSION   DADSYM010000197 DADSYM010000000
VERSION     DADSYM010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039579
            Sequence Read Archive: SRR6362533
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5689)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5689)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980162.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 03:39:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,484
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 4,347
            CDSs (with protein)               :: 4,347
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 43 of 85
            Pseudo Genes (incomplete)         :: 37 of 85
            Pseudo Genes (internal stop)      :: 19 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5689
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039579-rid11723173.denovo.199"
                     /strain="LGE2415"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8302 N 95.9628 W"
                     /collection_date="2015-08-24"
     gene            142..852
                     /locus_tag="J1Y83_004039"
     CDS             142..852
                     /locus_tag="J1Y83_004039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HBA7632132.1"
                     /translation="MQTTRTPYSVSFMATVLLLLLFACHSTIANAAVALGATRVIYPA
                     NQKQVLLPVTNNDPASVYLIQSWIENAGDQKDTQFVITPPLFSMQGKKENTLRIINAT
                     NHQLPGDRESLFWVNVKAIPAMEKDQKNENTLQLAIISRIKMFYRPTHLAMAPEEAPA
                     MLRFRRSGSKLTLINPTPYFITVTNMKAGNSNLPNTMVPPKGEVSVDIPHAVTGDISF
                     QTINDYGALTPRIKATMQ"
     gene            894..3545
                     /locus_tag="J1Y83_004040"
     CDS             894..3545
                     /locus_tag="J1Y83_004040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310137.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HBA7632133.1"
                     /translation="MTAFRAAFKAYRMHQVLILPRFARLTFALGLATAVFPVDAEYYF
                     NPRFLSNDLAESVDLSAFTKGREAPPGTYRVDIYLNDEFMASRDITFIADDNNADLIP
                     CLSTDLLVSLGIKKSALLDNKEHSADKHVPDNSACTPFQDRLADASSEFDVGQQHLSL
                     SVPQIYVGRMARGYVSPDLWEEGINAGLLNYSFNGNSINHRSNHNAGKSNYAYLNLQS
                     GINIGSWRLRDNSTWSYNSGTSNSSDSNKWQHINTSAERDIIPLRSRLTVGDSYTDGD
                     IFDSVNFRGLKINSTEAMLPDSQHGFAPVIHGIARGTAQVSVKQNGYDVYQTTVPPGP
                     FTIDDINSAANGGDLQVTIKEADGSIQTLYVPYSSVPVLQRAGYTRYALAMGEYRSGN
                     NLQSTPKFVQASLMHGLKGNWTPYGGMQIAEDYQAFNLGIGKDLGLFGAFSFDITQAN
                     TTLADDTRHSGQSVKSVYSKSFYQTGTNIQVAGYRYSTQGFYNLSDSAYSRMSGYTVK
                     PPTGDTSEQTLFIDYFNLFYSKRGQEQISISQQLGNYGTTFFSASRQSYWNTSRSDQQ
                     ISFGLNVPFGDITTSLNYSYSNNIWQNDRDHLLAFTLNVPFSHWMRTDSQSAFHNSNA
                     SYSMSNDLKGGMTNLSGVYGTLLPDNNLNYSVQVGNTHGGNTSSGTSGYSSLNYRGAY
                     GNTNVGYSRSGDSSQIYYGMSGGIIAHADGITFGQPLGDTMVLVKAPGADNVKIENQT
                     GIHTDWRGYAILPFATEYRENRVALNANSLADNVELDETVVTVIPTHGAIARATFNVQ
                     IGGKVLMTLKYGNKSVPFGAIVTHGENKNGSIVAENGQVYLTGLPQSGKLQVSWGKDK
                     NSNCIVEYKLPEVSPGTLLNQQTAICR"
     gene            3559..4089
                     /locus_tag="J1Y83_004041"
     CDS             3559..4089
                     /locus_tag="J1Y83_004041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HBA7632134.1"
                     /translation="MKYNNIIFLGLCLGLTTYSALSADSVIKISGRVLDYGCTVSSDS
                     LNFTVDLQKNSARQFPTTGSTSPAVPFQITLSECSKGTTGVRVAFNGIEDAENNTLLK
                     LDEGSNTASGLGIEILDGNMRPVKLNDLHAGMQWIPLVPDQNNILPYFARLKSTQKSV
                     NPGLVSASATFTLEFQ"
     gene            4102..4605
                     /locus_tag="J1Y83_004042"
     CDS             4102..4605
                     /locus_tag="J1Y83_004042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HBA7632135.1"
                     /translation="MKRLHKRFLLATFCTLFTATLQAADVTITVNGRVVAKPCTIQTK
                     EANVNLGDLYTRNLQQPGSASGWHNITLSLTDCPIETSTVTAIVTGSTDNTGYYKNEG
                     TAENIQIELRDDQDATLKNGDSKTVIVDEITRNAQFPLKARAITVNGNASQGTIEALI
                     NVIYTWQ"
     gene            4665..5579
                     /locus_tag="J1Y83_004043"
     CDS             4665..5579
                     /locus_tag="J1Y83_004043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HBA7632136.1"
                     /translation="MGKTFSIKVLFGIYLLLMAGKVFAFSCNVDGGSSIGAGTTSVYV
                     NLDPVIQPGQNLVVDLSQHISCWNDYGGWYDTDHINLVQGSAFAGSLQSYKGSLYWNN
                     VTYPFPLTTNTNVLDIGDKTPMPLPLKLYITPVGAAGGVVIKAGEVIARIHMYKIATL
                     GSGNPRNFTWNIISNNSVVMPTGGCTVDSRNVTVNLPDFPGSAEIPLGVYCSSEQKLS
                     FYLSGATTDSARQVFANTAPDATKASGVGVSLMRNGKTLATGENVSLGTVNKSKVPLG
                     LSATYGQTGNKVAAGAVQSVIGVTFIYE"
BASE COUNT         1644 a         1238 c         1249 g         1558 t
ORIGIN      
        1 ctatcaaaag agaaccaaca tttattacaa acgcattaag tctggaaacc cggcgactcg
       61 ccaatgcatt agcaatattt accgattgtc tgatgtaacg gtttccccgc ttatcctctt
      121 aacacatgga gaatgcatca gatgcaaact acgcgtaccc cctactctgt ttcctttatg
      181 gcaacagtgt tacttttatt gctctttgct tgccatagca ccattgccaa cgcagctgtc
      241 gcacttggtg cgacgcgggt aatttatccg gcaaaccaga aacaggtttt attgcctgtc
      301 acgaataatg atcctgccag tgtctatctc attcagtcat ggatagaaaa tgcgggtgac
      361 cagaaagata cccagtttgt cattacccca ccgctttttt ccatgcaagg taaaaaagag
      421 aatacgcttc ggataatcaa cgccactaat catcagttac caggcgaccg tgaaagcctg
      481 ttctgggtta atgttaaagc tatccctgct atggaaaaag accagaagaa tgagaataca
      541 ttacagctgg caattattag ccgcataaaa atgttttatc gccccacgca tctggcaatg
      601 gcacctgaag aagcgcctgc aatgcttcgg tttcgtcgca gcggtagcaa actcactctt
      661 attaatccaa cgccctactt cattaccgta acaaatatga aagcaggaaa tagcaatctc
      721 ccaaacacca tggtgccgcc aaaaggtgaa gtatcggtgg atatcccgca tgctgtaacc
      781 ggggatatca gttttcagac aatcaatgat tacggtgcat taactccgcg tattaaggca
      841 accatgcagt aagccaatga cctaattaat aataaactca atcgggatta aaaatgacag
      901 cctttcgtgc cgcatttaag gcatatcgta tgcatcaggt gttaatactg cctcgctttg
      961 cacggcttac ctttgccctt ggcttagcaa ctgcggtttt tcccgttgac gcagagtatt
     1021 attttaatcc acggttttta agcaatgacc ttgctgaatc tgtcgattta tctgccttta
     1081 ctaagggacg cgaggcccca cctggtacat atcgggtcga catttatttg aacgacgaat
     1141 ttatggctag tcgggatatt acttttattg cagatgataa taacgcagat ctcatcccat
     1201 gcctgagtac agaccttctt gtcagtcttg gcataaaaaa atcagcatta ttagacaaca
     1261 aggaacattc ggcagataaa catgtgccgg acaacagcgc atgcacacca tttcaggatc
     1321 gtttggctga cgcatcatct gagtttgatg ttggtcagca acatctgtca ctctccgttc
     1381 cgcaaatata tgtcggcaga atggctcgcg gctatgtttc tcctgatctg tgggaagaag
     1441 gaataaatgc cgggctacta aactatagtt ttaatggtaa ttctattaat caccgtagta
     1501 accataatgc aggaaaatcc aactatgcat atttgaattt acagagtggc atcaacattg
     1561 gtagttggcg actgcgcgat aactcaacgt ggagttataa cagtgggact agcaattcat
     1621 ctgacagcaa taaatggcag catatcaata cgtcggctga acgtgacatt attccgttac
     1681 gctcacggtt aacagtgggt gatagttata ccgatggcga tatttttgac agtgtgaact
     1741 ttcgtggcct taaaataaat tcaacagaag cgatgttgcc cgatagccaa catggtttcg
     1801 ctccggtgat tcatggtatt gctcgtggca ccgcacaagt gagtgtaaaa cagaatggat
     1861 acgatgttta tcagactact gtcccacccg gcccttttac tattgatgat atcaactctg
     1921 cggccaatgg tggtgatctg caagtaacca taaaagaggc agacggcagt attcagacat
     1981 tatatgttcc ttattcgtct gttccggttc tccaacgtgc tggatatacg cgttatgcac
     2041 ttgccatggg ggaatatcgt agtggaaata acctgcaaag cacccccaag ttcgtacaag
     2101 caagcttgat gcatggtctg aaaggaaact ggacacctta tggcggaatg caaattgcag
     2161 aagattatca ggccttcaac cttggtattg gtaaagattt aggacttttt ggtgcctttt
     2221 ctttcgatat tacgcaggcc aatacgacac ttgcagatga cacccgtcac agcggacaat
     2281 cggttaaatc cgtctacagc aaatccttct accagacggg aaccaatatc caggtcgcag
     2341 gatatcgcta ttctacgcaa ggtttttata acttatccga cagtgcatac agtcgaatga
     2401 gtggttacac cgtcaagcct cctaccggag acaccagtga gcagacacta tttattgatt
     2461 attttaatct gttctacagt aagcgtggtc aggaacaaat aagcatctct cagcagcttg
     2521 gaaattacgg tacgacattt ttcagtgcca gtcgccaaag ttactggaac acgtcacgca
     2581 gcgaccagca aatatcattt ggattaaatg tgccgtttgg tgatattacg acttcgctga
     2641 attacagcta ttccaataat atatggcaaa acgatcggga tcatttactc gcttttacgc
     2701 ttaatgttcc ctttagtcat tggatgcgta cagatagtca gtcggcattt cataattcaa
     2761 acgccagtta cagtatgtca aacgatttga aaggcggcat gaccaaccta tcaggggttt
     2821 atggcactct actgccggat aataacctga attatagtgt tcaggtcggt aacacccacg
     2881 ggggtaatac atcgtctggc accagtggtt acagttctct taattatcgt ggagcttacg
     2941 gtaatacgaa tgtcggttac agtcggagtg gtgacagcag ccagatttat tacggaatga
     3001 gtggtggcat tattgctcat gctgatggca tcacctttgg acagccgctg ggcgacacaa
     3061 tggttctggt taaggctcct ggcgctgata atgtcaaaat agagaaccag accggtattc
     3121 ataccgactg gcgtggctat gccatattac catttgcgac agaatataga gaaaatcgtg
     3181 tcgctcttaa cgcgaattcc cttgcagata atgttgaact ggatgaaacc gtagtcactg
     3241 tcatcccaac tcacggtgct attgccagag caacatttaa tgtacaaatc ggcgggaaag
     3301 tattaatgac gttgaagtac ggtaataaga gcgttccatt cggtgcaatt gtcacacatg
     3361 gagagaataa aaatggcagc attgtcgcgg aaaacggtca ggtttatctg actggacttc
     3421 cacagtcagg gaaattacag gtttcatggg gcaaagataa aaactcaaac tgtattgtcg
     3481 agtacaagct tcctgaagtt tctcctggta ccttactgaa ccagcagaca gcaatctgtc
     3541 gctaaggaac aatagattat gaaatacaat aacattattt tcctcggttt atgtctgggg
     3601 ttaaccacct attctgcttt atccgcagat agtgttatta aaattagcgg gcgcgtcctc
     3661 gattatggct gcacagtctc atcggattcg cttaatttta ccgtagatct ccaaaaaaac
     3721 agtgccagac aatttccaac gaccggtagc acaagtccag ccgtcccttt tcagattacg
     3781 ttaagtgaat gcagtaaagg gacaacgggg gttcgggttg catttaacgg tattgaggat
     3841 gcagaaaata atactctgtt gaaactagat gaaggaagca atacggcctc cggtttgggt
     3901 atagaaatac tggacggaaa tatgcgtccg gtgaaattga atgaccttca tgccgggatg
     3961 cagtggatcc cattggtacc agatcagaac aatattttgc cttacttcgc tcgtttgaag
     4021 tcaactcaga agtccgtcaa tccaggactg gtgagcgctt cggcaacatt tacccttgaa
     4081 tttcaataat cagagtcaga tatgaaaaga cttcacaaga ggttcctgtt agctacgttt
     4141 tgcacgttat tcacagcaac tctccaggcc gccgatgtca ctatcactgt taatggtcgg
     4201 gtagtcgcta aaccatgcac tattcaaacc aaagaagcta acgttaatct cggggatctt
     4261 tatacacgca atctgcaaca acctggttct gcatctggct ggcacaatat tactttgtca
     4321 ttaaccgatt gtccgattga aacaagtaca gtgacggcaa tcgtgacagg ttcaactgac
     4381 aatacgggtt attacaaaaa tgaaggtact gccgaaaata ttcagataga gctaagggat
     4441 gaccaggatg cgacgttaaa aaatggcgat agcaaaacgg ttattgttga tgagatcact
     4501 cgtaatgcac agtttccact taaggcaaga gctatcacgg tgaatggaaa cgcaagccag
     4561 ggtacgatcg aggcgctaat caatgtgatc tatacctggc aataacatca atttgatttt
     4621 attttcctcc tttttaaatc aattttttaa ccttaggata tatcatgggt aaaacattca
     4681 gtataaaggt cctgttcggt atttatcttc tattaatggc aggcaaagtt tttgccttca
     4741 gttgtaatgt cgatggcggg tcaagtattg gagcaggtac aacctctgtt tatgttaatc
     4801 tcgatcctgt aatacaaccg ggccagaatc tggttgtaga cttgtctcag catataagtt
     4861 gctggaatga ttacggcggc tggtacgaca ctgatcatat aaacctggta caaggatcag
     4921 ctttcgcagg ctccctacag tcatataaag gatcactgta ctggaataat gtgacctacc
     4981 ctttcccgtt gacgacaaat accaacgtat tggatattgg tgataagaca ccgatgccat
     5041 taccgttaaa actctatatt accccagtcg gagcggctgg tggagttgtc ataaaagcag
     5101 gggaagttat tgcccgtatt catatgtata aaattgccac gttaggaagc ggaaatccgc
     5161 gtaattttac gtggaatatt atttcaaata acagtgttgt tatgccaaca ggcggttgca
     5221 ccgttgatag tcgtaatgtc accgtgaatc tcccggactt tcctggaagc gcagaaatac
     5281 cccttggtgt atattgctct agcgaacaaa agctctcatt ttatctttcg ggtgccacca
     5341 ctgacagtgc ccgacaagtg tttgctaaca cagcaccaga tgcaacaaaa gcatcgggcg
     5401 tgggtgtctc tctgatgcgt aatggaaaaa cactggcaac aggagaaaat gtctccctgg
     5461 ggacagtaaa taaaagcaaa gtacctctgg gtttaagcgc aacctatgga caaacaggaa
     5521 acaaggttgc agccggagcc gtccagtcag tcattggtgt cacttttatc tatgaataat
     5581 tgtgattttg acattagtct atgcgctcca ttcatgaaaa ttgcgcggat aattattttg
     5641 tgaaggctat tatcctacac ttgtaagaaa atccgcgcac aattgaagc
//
LOCUS       DADSYM010000198         5632 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2415
            SAMN08039579-rid11723173.denovo.200, whole genome shotgun sequence.
ACCESSION   DADSYM010000198 DADSYM010000000
VERSION     DADSYM010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039579
            Sequence Read Archive: SRR6362533
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5632)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5632)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980162.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 03:39:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,484
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 4,347
            CDSs (with protein)               :: 4,347
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 43 of 85
            Pseudo Genes (incomplete)         :: 37 of 85
            Pseudo Genes (internal stop)      :: 19 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5632
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039579-rid11723173.denovo.200"
                     /strain="LGE2415"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8302 N 95.9628 W"
                     /collection_date="2015-08-24"
     gene            114..455
                     /locus_tag="J1Y83_004044"
     CDS             114..455
                     /locus_tag="J1Y83_004044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418020.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA7632137.1"
                     /translation="MKTSKTVAKLLFVVGALVYLVGLWISCPLLSGKGYFLGVLMTAT
                     FGNYAYLRAEKLGQLDDFFIHVCQLVALITIGLLFIGVLNAPINAYEMVIYPIAFFVC
                     LFGQMRLFRSA"
     gene            501..938
                     /locus_tag="J1Y83_004045"
     CDS             501..938
                     /locus_tag="J1Y83_004045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiaB family inner membrane protein"
                     /protein_id="HBA7632138.1"
                     /translation="MDNKISTYSPAFSIVSWVALIGGIVTYLLGLWNAEMQLNEKGYY
                     FAVLVLGLFSAASYQKTVRDKYEGIPTTPIYYMTCLTVFIISVALLLVGLWNATLLLS
                     EKGFYGLAFFLSLFGAVAVQKNIRDAGINPPKETQVTQEEYSE"
     gene            complement(979..1974)
                     /locus_tag="J1Y83_004046"
     CDS             complement(979..1974)
                     /locus_tag="J1Y83_004046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HBA7632139.1"
                     /translation="MQPKIYWIDNLRGIACLMVVMIHTTTWYVTNAHSVSPITWDIAN
                     VLNSASRVSVPLFFMISGYLFFGERSAQSRHFLRIGLCLFFYSAIALLYIALFTSINV
                     ELALKNLLQKPVFYHLWFFFAIAVIYLVSPLIQVKNVGGKMLLVLMVVIGIIANPNTV
                     PQKIDGFEWLPINLYINGDTFYYILYGMLGRAIGMMDTQHKALSWVSAALFATGVFII
                     SRGTLYELQWRGNFADTWYLYCGPMVFICAIALLTLVKNTLYTRTICGLGLISRHSLG
                     IYGFHALIIHALRTRGIELKNWPILDIIWIFCATLAASLLLSMLVQRIDRNRLVS"
     gene            2149..2448
                     /locus_tag="J1Y83_004047"
     CDS             2149..2448
                     /locus_tag="J1Y83_004047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA7632140.1"
                     /translation="MMMNAFFPAMALIVLVGCSTPPPVQKAQRVKVDPLRSLNMEALC
                     KDQAAKRYNTGEQKIDVTAFEQFQGSYEMRGYTFRKEQFVCSFDADGHFLHLSMR"
     gene            2543..3454
                     /gene="glyQ"
                     /locus_tag="J1Y83_004048"
     CDS             2543..3454
                     /gene="glyQ"
                     /locus_tag="J1Y83_004048"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HBA7632141.1"
                     /translation="MQKFDTRTFQGLILTLQDYWARQGCTIVQPLDMEVGAGTSHPMT
                     CLRALGPEPMAAAYVQPSRRPTDGRYGENPNRLQHYYQFQVVIKPSPDNIQELYLGSL
                     KELGMDPTIHDIRFVEDNWENPTLGAWGLGWEVWLNGMEVTQFTYFQQVGGLECKPVT
                     GEITYGLERLAMYIQGVDSVYDLVWSDGPLGKTTYGDVFHQNEVEQSTYNFEYADVDF
                     LFTCFEQYEKEAQQLLALENPLPLPAYERILKAAHSFNLLDARKAISVTERQRYILRI
                     RTLTKAVAEAYYASREALGFPMCNKDK"
     gene            3464..5533
                     /gene="glyS"
                     /locus_tag="J1Y83_004049"
     CDS             3464..5533
                     /gene="glyS"
                     /locus_tag="J1Y83_004049"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HBA7632142.1"
                     /translation="MSEKTFLVEIGTEELPPKALRSLAESFAANFTAELDNAGLAHGT
                     VQWFAAPRRLALKVANLAEAQPDREIEKRGPAIAQAFDAEGKPSKAAEGWARGCGITV
                     DQAERLTTDKGEWLLYRAHVKGESTEALLPNMVATSLAKLPIPKLMRWGASDVHFVRP
                     VHTVTLLLGDKVIPATILGIQSDRVIRGHRFMGEPEFTIDNADQYPEILRERGKVIAD
                     YEERKAKIKADAEEAARKIGGNADLSESLLEEVASLVEWPVVLTAKFEEKFLAVPSEA
                     LVYTMKGDQKYFPVYANDGKLLPNFIFVANIESKDPQQIISGNEKVVRPRLADAEFFF
                     NTDRKKRLEDNLPRLQTVLFQQQLGTLRDKTDRIQALAGWIAEQIGADVNHATRAGLL
                     SKCDLMTNMVFEFTDTQGVMGMHYARHDGEAEDVAVALNEQYQPRFAGDDLPSNPVAC
                     ALAIADKMDTLAGIFGIGQHPKGDKDPFALRRAALGVLRIIVEKNLNLDLQTLTEEAV
                     RLYGDKLTNANVVDDVIDFMLGRFRAWYQDEGYTVDTIQAVLARRPTRPADFDARMKA
                     VSHFRTLEAAAALAAANKRVSNILAKSDEVLSDRVNASTLKEPEEIKLAMQVVVLRDK
                     LEPYFAEGRYQDALVELAELREPVDAFFDKVMVMVDDKELRINRLTMLEKLRELFLRV
                     ADISLLQ"
BASE COUNT         1439 a         1354 c         1433 g         1406 t
ORIGIN      
        1 attaattaat atttagtatg aatttattct gaaaatcatt tgttaatggc atttttcagt
       61 tttgtctttc gttggttact cgtaatgtat cgctggtaga tatggagatc gttatgaaaa
      121 cctcaaagac tgtggcaaaa ctattatttg ttgtcggggc gctggtttat ctggttgggc
      181 tatggatctc atgcccattg ttaagtggaa aaggctattt tcttggcgtg ttaatgacgg
      241 caacttttgg caactatgca tatcttcgcg cagaaaaact tgggcaactg gatgattttt
      301 ttatccacgt ctgtcagttg gtagcgttaa tcactatcgg tctcttgttt atcggtgttt
      361 taaacgcacc tatcaatgct tatgaaatgg tgatctatcc catcgccttt tttgtctgct
      421 tgtttggtca aatgcgtttg tttcgctcgg catgagcaac ataaagttct tacacattca
      481 ggaatgaaag gaatactgtg atggacaaca aaatatcaac ctattctccg gcatttagta
      541 ttgtttcatg ggtagccctt attggcggta ttgttaccta tctgttagga ctatggaatg
      601 cagagatgca gttaaatgaa aaagggtatt attttgccgt actggtatta ggtttgtttt
      661 ccgcggcgtc ttatcaaaag accgttcggg acaagtatga aggcatacct accaccccca
      721 tttattatat gacttgcctg acggtcttta ttatctctgt tgcgttgttg ctggtaggtc
      781 tatggaatgc aacgttatta ctcagcgaaa aaggctttta tgggctggct ttcttcttaa
      841 gcttgtttgg tgcagtagcg gtgcagaaga atattcgtga tgccggaata aacccaccaa
      901 aagaaacaca ggttacccaa gaagaataca gcgaataatt cacataagcc cggttagtcc
      961 aatgtaaccg ggcttttact aactcactaa tctgtttctg tcgattcgtt gtaccagcat
     1021 agaaagtaac aaactcgctg ccaacgtcgc gcaaaagatc caaataatat cgagtattgg
     1081 ccagttttta agctcaatcc cccgggtgcg cagtgcatgg ataatcaatg catggaaccc
     1141 gtatatacct aacgaatggc gggagattaa gccaagtccg caaatggtac gcgtatacag
     1201 cgtgttttta accagagtca ataacgcgat tgcgcagata aaaaccatcg gcccacagta
     1261 aagataccag gtatcggcaa aatttccgcg ccactgtaat tcatataatg tcccgcgaga
     1321 gataataaaa acccccgtcg caaacagtgc ggcgctcacc cacgacagtg ctttatgctg
     1381 tgtgtccatc atccctatag cgcggcccaa catgccataa aggatgtagt aaaaagtatc
     1441 gccattgata tataagttaa ttggcagcca ttcaaaaccg tcgattttct gcggcacagt
     1501 atttgggtta gcgatgatac caatcaccac cattagcacc agcaacattt ttccgccgac
     1561 gttcttcacc tgaatcagcg gtgaaaccag ataaatcact gcaatcgcga agaaaaacca
     1621 taaatggtaa aacactggct tttgcagcag gtttttcagc gctaactcca cattgatgga
     1681 ggtaaacagc gcaatgtaga gcagcgcgat tgcgctataa aaaaacaggc ataagccgat
     1741 acgcaagaaa tggcgcgact gggcgctgcg ttcgccaaaa aagagatagc cggaaatcat
     1801 gaaaaatagc ggcacgctga cacgagaggc agaattcaga acattggcga tatcccaggt
     1861 gatggggcta acactatgag cattggtcac ataccaggta gtggtgtgaa tcatcaccac
     1921 cattaaacac gctatccctc gcaggttatc aatccagtaa attttgggct gcatctgtgt
     1981 ctctgtatct ggttaaaaaa agtctgaccg ataaatcatt cagttggcgc actggaataa
     2041 tctgagtttt atcactacag cttatagagg cttaaggaaa ttcgtaagat atcagccact
     2101 ataccgatat aaataataag actcacctgc aaaccagacg gtaatttaat gatgatgaac
     2161 gctttctttc cggcaatggc gcttattgtg ctagtgggtt gttctacacc gccacccgtg
     2221 cagaaagcac aacgggtaaa ggttgatcct ctgcgttcgt tgaatatgga agcgttatgt
     2281 aaggatcagg cggcaaaacg ttataacacc ggcgagcaaa aaatcgacgt caccgccttc
     2341 gaacagttcc agggaagcta tgaaatgcgc ggttatacct tccggaaaga gcagtttgtc
     2401 tgttcttttg acgcggatgg ccattttttg catctttcca tgcgttaagc cctgcttttt
     2461 cccgtttcgt actgtatatc ttccatccag cgggtatact gatcccttcc tttaaatcca
     2521 cacgtatcca gcacgaaata atatgcaaaa gtttgatacc aggaccttcc agggcttgat
     2581 cctgacctta caggattact gggctcgcca gggctgcacc attgttcaac cattggacat
     2641 ggaagtcggc gcgggaacct ctcacccaat gacctgtctg cgcgcgctgg ggccagaacc
     2701 gatggcggct gcttatgttc agccttctcg tcgcccgacc gatggtcgct acggcgaaaa
     2761 ccccaaccgt ttacagcact actatcagtt ccaggtggtc attaagccat cgccggacaa
     2821 tattcaggag ctgtacctcg gttctctgaa agagctgggc atggacccga ccatccacga
     2881 catccgtttc gtggaagata actgggaaaa cccgacgctg ggtgcctggg ggctgggctg
     2941 ggaagtgtgg ctaaacggca tggaagtgac gcagttcact tacttccagc aggttggtgg
     3001 tctggaatgt aaaccggtta ccggtgagat cacctacggt ctggaacgtc tggccatgta
     3061 cattcagggc gtagacagcg tttacgacct ggtctggagc gacggcccgc tgggtaaaac
     3121 cacctacggc gacgtgttcc atcagaacga agtggagcag tccacttaca acttcgaata
     3181 cgcggatgtg gacttcctgt tcacctgctt cgagcagtac gagaaagaag cgcagcaact
     3241 gctggcgctg gaaaatccgc tgccgctgcc agcctacgag cgtattctga aagccgccca
     3301 cagcttcaac ctgctggatg cgcgtaaagc catctccgtc accgagcgtc agcgctatat
     3361 tctgcgcatt cgcaccctga ccaaagcagt ggcagaagca tactacgctt cccgtgaagc
     3421 cctcggcttc ccgatgtgca acaaagataa gtaagaggcg gctatgtctg agaaaacttt
     3481 tctggtggaa atcggcactg aagagctgcc accaaaagca ctgcgcagcc tggctgagtc
     3541 ctttgctgcg aactttactg cggagctgga taacgctggc ctcgcacacg gcaccgttca
     3601 atggtttgct gctccgcgtc gtctggcgct gaaagtagct aacctggcgg aggcgcaacc
     3661 ggatcgcgaa atagaaaaac gcggcccggc aattgcccag gcgttcgacg ctgaaggcaa
     3721 accgagcaaa gcggcagaag gttgggcgcg tggttgcggt attaccgttg accaggctga
     3781 gcgtctgact accgataaag gcgaatggct gctgtatcgc gcccatgtga agggtgaaag
     3841 caccgaagca ctgctgccga atatggttgc gacttcgctg gcgaaacttc cgatcccgaa
     3901 actgatgcgt tggggcgcgt ctgacgtaca cttcgtgcgt ccggttcaca ctgtaaccct
     3961 gctgctgggc gacaaagtta ttccggctac cattctgggt attcagtccg atcgcgtgat
     4021 tcgcggccac cgctttatgg gcgagccgga attcactatc gacaatgccg atcagtatcc
     4081 ggaaattctg cgtgagcgcg ggaaagtcat cgccgattac gaagaacgta aggcgaagat
     4141 taaagccgat gctgaagaag cagcacgtaa gattggcggt aacgctgatt taagcgaaag
     4201 cctgctggaa gaagtggctt cgctggtgga gtggccggtc gttctgaccg cgaaattcga
     4261 agagaaattc ctcgcggtgc cgtctgaagc actggtttac accatgaaag gtgaccagaa
     4321 gtacttcccg gtgtatgcga acgacggcaa actgctgccg aactttatct tcgttgccaa
     4381 catcgaatcg aaagatccgc agcagattat ctccggtaac gagaaagtcg ttcgtccacg
     4441 tctggcggat gccgagttct tcttcaatac cgaccgtaaa aaacgtcttg aagataatct
     4501 gccgcgcctg caaaccgtgt tgttccagca acagttgggg acgctgcgcg acaaaactga
     4561 ccgcattcag gcgctggctg gctggattgc tgagcagatt ggcgctgacg ttaaccacgc
     4621 tacccgtgcg ggcctgctgt ctaagtgtga cctgatgacc aacatggtct tcgagttcac
     4681 cgacacccag ggcgttatgg ggatgcacta cgcgcgtcac gatggcgaag cggaagacgt
     4741 agctgtcgcg ctgaatgagc agtatcagcc gcgctttgcc ggtgatgacc tgccgtctaa
     4801 cccggtagcc tgtgcgctgg cgattgctga caagatggat accctggcgg gtatcttcgg
     4861 tatcggccag catccgaaag gcgacaaaga cccttttgcg ctgcgtcgtg ccgcacttgg
     4921 cgtgctgcgt attatcgttg agaagaacct caaccttgat ctgcaaacgc tgaccgaaga
     4981 agcagtgcgt ctgtatggcg ataagctgac taatgccaac gtggttgatg atgttatcga
     5041 ctttatgctc ggtcgcttcc gcgcctggta tcaggacgaa ggttacaccg ttgataccat
     5101 ccaggcggta ctggcgcgtc gtccgactcg tccggctgat ttcgatgccc gtatgaaagc
     5161 ggtatcgcac ttccgtaccc tggaagcagc tgcagcactg gcggcggcga acaagcgtgt
     5221 atctaacatc ctggcgaaat ctgatgaagt gctgagcgat cgcgtgaatg cctctactct
     5281 gaaagagccg gaagaaatta aactggcgat gcaggttgtg gtgctacgtg acaagctgga
     5341 gccgtacttt gcggaaggtc gttaccagga tgcgctggtc gaactggctg agctgcgtga
     5401 accggtcgat gctttcttcg ataaagtgat ggtcatggtt gatgacaaag aattgcgaat
     5461 caaccgtctt accatgctgg agaaactgcg cgaattattc ctgcgcgttg cggatatttc
     5521 gctgttacag taataatgcc gttattaaat agcctgccat ctggcaggct ttttttatcg
     5581 ctaaataata cagcaacctt taataatctt ctgatgaata aagattatct ca
//
LOCUS       DADSYM010000199         5505 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2415
            SAMN08039579-rid11723173.denovo.201, whole genome shotgun sequence.
ACCESSION   DADSYM010000199 DADSYM010000000
VERSION     DADSYM010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039579
            Sequence Read Archive: SRR6362533
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5505)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5505)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980162.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 03:39:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,484
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 4,347
            CDSs (with protein)               :: 4,347
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 43 of 85
            Pseudo Genes (incomplete)         :: 37 of 85
            Pseudo Genes (internal stop)      :: 19 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5505
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039579-rid11723173.denovo.201"
                     /strain="LGE2415"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8302 N 95.9628 W"
                     /collection_date="2015-08-24"
     gene            224..1438
                     /locus_tag="J1Y83_004050"
     CDS             224..1438
                     /locus_tag="J1Y83_004050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-galactonate dehydratase family protein"
                     /protein_id="HBA7632143.1"
                     /translation="MKIIAVDVFVTCPGRNFVTLKITTESGLCGLGDATLNGRELSVA
                     SYLKDHLCPQLIGRDASRIEDIWQFFYKGAYWRRGPVTMSAISAIDMALWDIKAKAAN
                     MPLYQLLGGASREGVMVYCHTTGRTIDEVLEDYAKHQQMGFKAIRVQCGVPGMQTTYG
                     LAKGKGLAYEPATKGLWPEEQLWSSEKYLDFTPKLFEAVRNKFGFNEHLLHDMHHRLT
                     PIEAARFGRSIEDYRLFWMEDPTPAENQECFRLIRQHTVTPIAVGEVFNSIWDCKQLI
                     EEQLIDYIRTTITHAGGITGMRRIADFASLYQVRTGSHGPSDLSPVCHAAALHFDLWV
                     PNFGVQEYMGYSEQMLEVFPHSWRFDEGYMHPGDEPGLGISFDEKLAAKYPYDPAYLP
                     VARLEDGTLWNW"
     gene            1505..2788
                     /locus_tag="J1Y83_004051"
     CDS             1505..2788
                     /locus_tag="J1Y83_004051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000382182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBA7632144.1"
                     /translation="MDVDVSTSVAGNKPQRIRRIQTVTLVLLFMAGIVNFLDRSSLSV
                     AGEAIRGELGLSATEFGVLLSAFSLSYGFSQLPSGILLDRFGPRIVLGAGLIFWSLMQ
                     ALTGMVNSFSHFILMRIGLGIGEAPFMPAGVKSITDWYAQKERGTALGIFNSSTVIGQ
                     AIAPPALVLMQLAWGWRTMFVIIGVAGILVGICWYAWYRNRAQFVLTEEERTYLSAPV
                     KPRPQLQFSEWLALFKHRTTWGMILGFSGVNYTGWLYIAWLPGYLQAEQGFSLAKTGW
                     VAAIPFLAAAVGMWVNGIVVDRLAKKGYDLAKTRKTAIVCGLMMSALGTLLVVQSSSP
                     AQAVAFISMALFCVHFAGTSAWGLVQVMVSETKVASIAGIQNFGSFVFASFAPIVTGW
                     VVDTTHSFNLALVIAACVTFTGALCYFFIVKDRIE"
     gene            3040..3957
                     /gene="oxyR"
                     /locus_tag="J1Y83_004052"
     CDS             3040..3957
                     /gene="oxyR"
                     /locus_tag="J1Y83_004052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator OxyR"
                     /protein_id="HBA7632145.1"
                     /translation="MNIRDLEYLVALAEHRHFRRAADSCHVSQPTLSGQIRKLEDELG
                     VMLLERTSRKVLFTQAGMLLVDQARTVLREVKVLKEMASQQGETMSGPLHIGLIPTVG
                     PYLLPHIIPMLHQTFPKLEMYLHEAQTHQLLAQLDSGKLDCVILALVKESEAFIEVPL
                     FDEPMLLAIYEDHPWANRECVPMADLAGEKLLMLEDGHCLRDQAMGFCFEAGADEDTH
                     FRATSLETLRNMVAAGSGITLLPALAVPPERKRDGVVYLPCIKPEPRRTIGLVYRPGS
                     PLRSRYEQLAEAIRARMDGHFDKVLKQAV"
     gene            complement(3940..5340)
                     /gene="sthA"
                     /locus_tag="J1Y83_004053"
     CDS             complement(3940..5340)
                     /gene="sthA"
                     /locus_tag="J1Y83_004053"
                     /EC_number="1.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418397.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Si-specific NAD(P)(+) transhydrogenase"
                     /protein_id="HBA7632146.1"
                     /translation="MPHSYDYDAIVIGSGPGGEGAAMGLVKQGARVAVIERYQNVGGG
                     CTHWGTIPSKALRHAVSRIIEFNQNPLYSDHSRLLRSSFADILNHADNVINQQTRMRQ
                     GFYERNHCEILQGNARFVDEHTLALDCPDGSVETLTAEKFVIACGSRPYHPTDVDFTH
                     PRIYDSDSILSMHHEPRHVLIYGAGVIGCEYASIFRGMDVKVDLINTRDRLLAFLDQE
                     MSDSLSYHFWNSGVVIRHNEEYEKIEGCDDGVIMHLKSGKKLKADCLLYANGRTGNTD
                     SLALQNIGLETDSRGQLKVNSMYQTAQPHVYAVGDVIGYPSLASAAYDQGRIAAQALV
                     KGEANAHLIEDIPTGIYTIPEISSVGKTEQQLTAMKVPYEVGRAQFKHLARAQIVGMN
                     VGTLKILFHRETKEILGIHCFGERAAEIIHIGQAIMEQKGGGNTIEYFVNTTFNYPTM
                     AEAYRVAALNGLNRLF"
BASE COUNT         1234 a         1263 c         1497 g         1511 t
ORIGIN      
        1 ttcattttta atgattaatg gagtttgtta tgtgattatt taacgtgttg ttttaacgat
       61 aatggctaat tattcaggtg gtttttgagg gtgttttttc tcctcggtag aaatttttat
      121 ttttaatgtg atcacggttg aataatcacc ttttctctct tgtatggtag taggcgtaaa
      181 attaagtttg aagtgtctat ttacaataca ggagattgca ataatgaaaa ttattgctgt
      241 ggatgttttt gttacttgtc ccgggcgtaa tttcgttacg cttaaaataa ccactgaaag
      301 cggactttgt ggattaggag atgccacgtt aaatggtcgc gaattgtcgg tcgcttctta
      361 tctgaaagat catctttgtc ctcaattaat tggccgcgat gccagccgta tcgaagatat
      421 ctggcaattc ttttataaag gagcttactg gcgacgtggt cccgtcacga tgtcggccat
      481 ttctgccatt gatatggcgt tgtgggatat caaagctaaa gcggcaaata tgccattgta
      541 tcaactgctt ggtggcgctt ctcgtgaagg cgtgatggtt tattgccaca ccaccgggcg
      601 gacgattgat gaagtccttg aagactatgc aaagcaccag cagatgggct ttaaggcgat
      661 tcgtgtgcag tgtggtgtac cgggtatgca aaccacctat ggtctggcga agggcaaagg
      721 actggcgtat gaaccagcaa ccaaaggcct gtggccagaa gagcagttgt ggtcttcaga
      781 aaagtatctc gattttacgc cgaagttatt tgaagcggtg cgtaataagt tcggttttaa
      841 cgaacatctg ttgcatgaca tgcaccatcg cctgaccccc atcgaagcgg cgcgctttgg
      901 gaggagcatt gaggattacc gcctgttctg gatggaagat ccgacgcctg ccgagaacca
      961 ggaatgtttt cgtctgattc gccagcatac cgtgacgccg attgcggtcg gtgaagtgtt
     1021 taacagtatc tgggactgca aacagttaat cgaagagcag ctgattgact atattcgcac
     1081 gacgattacc catgcaggcg gcattaccgg gatgcgacgg attgccgatt ttgcatcgct
     1141 ttatcaggta cgtaccggtt cgcatggccc ttcagattta tcgcctgttt gccatgcggc
     1201 ggcgctgcat tttgacctct gggtgccaaa ctttggcgtt caggagtaca tgggctattc
     1261 cgaacaaatg cttgaagtct tcccgcacag ctggcgcttc gatgaaggtt atatgcaccc
     1321 aggcgatgaa ccggggctgg gtatttcttt cgatgagaaa ctggcggcga aatatccgta
     1381 tgacccggcg tatctccccg tcgcacgact ggaagatggc acattgtgga actggtaata
     1441 gcggttcgct taattatttt ctgaatattc atcaaatcga ttaattccgc tcaaggagct
     1501 aaaaatggac gttgatgtgt caaccagtgt tgcgggtaat aaaccgcaac ggattcgtcg
     1561 tattcagaca gtgaccctgg ttttattatt tatggcgggg atcgttaatt ttctcgaccg
     1621 ttcgtcattg agcgtggcag gtgaagcaat tcgtggcgag ttaggattat ccgctaccga
     1681 gtttggtgtt ttgctttctg cattttctct gtcttatggt ttttcacaac ttccttccgg
     1741 tattttgcta gaccgctttg gcccacgaat agtgttaggc gcaggcttaa tattctggtc
     1801 attaatgcag gcattaacag gaatggttaa ttcttttagc cactttattt taatgcgtat
     1861 cggtctgggt attggcgaag cgccatttat gcctgcggga gtaaagtcga tcaccgactg
     1921 gtatgcgcaa aaagaacgcg gcacggcgct ggggattttt aactcatcta ccgttatcgg
     1981 tcaggctatc gcgcctcctg ctcttgtatt gatgcaactg gcatggggct ggcggacgat
     2041 gtttgtcatc atcggcgtag cagggattct ggttgggatc tgttggtacg cgtggtatcg
     2101 caaccgggcg cagtttgtcc tgaccgagga agaacggaca tatctctccg cgccggttaa
     2161 accgcgtcca cagttacaat ttagcgagtg gctggcgctg tttaagcatc ggacaacctg
     2221 ggggatgatt ttaggtttct ctggcgtcaa ttataccggt tggctctaca tcgcgtggtt
     2281 acccggttat ttgcaggccg aacaaggttt cagtctggcg aaaaccggtt gggtggcggc
     2341 aattcctttc ctcgcggcgg cggtcgggat gtgggttaac ggtattgttg tcgatcggct
     2401 ggcgaaaaaa ggctatgacc tggcgaagac gcgtaaaacg gcgattgtct gcggtttgat
     2461 gatgtcggca ttaggcacgt tgctggtcgt gcaatcttcc tcgccagccc aggcggtagc
     2521 gtttatctca atggcgctgt tctgcgtgca tttcgcggga acatctgcat gggggctggt
     2581 gcaggtgatg gtgtcagaaa caaaagtggc ttccatcgcc gggattcaaa actttggcag
     2641 ttttgttttt gcttcctttg ctccgatcgt aaccggttgg gtcgtggata ccacacactc
     2701 gtttaatctg gcgctggtta tcgcggcctg tgtgacgttc accggggcgc tgtgttactt
     2761 ctttattgtc aaagatcgga ttgagtaacc gttactatca ggctctcttg ctgtgggcct
     2821 gtagaataaa aaaaagcgga tcctggagat ccgcaaaagt tcacgttggc tttagttatt
     2881 cgagttgaga aactctcgaa acgggcagtg acttcaaggg ttaaaagagg tgccgctccg
     2941 tttctgtgag caattatcag tcagaatgct tgatagggat aatcgttcgt tgctatgcta
     3001 cctatcgccg cgaactatcg tggcaatgga ggatggataa tgaatattcg tgatcttgag
     3061 tacctggtgg cattggccga acaccgccat tttcggcgtg cggcagattc ctgccacgtt
     3121 agccagccga cgcttagcgg gcaaattcgt aagctggaag atgagttggg cgtgatgttg
     3181 ctggagcgga ccagccgtaa agtgttgttc acccaggcgg gaatgctgct ggtggatcag
     3241 gcgcgtaccg tactgcgtga ggtgaaagtc cttaaagaga tggcaagcca gcagggcgag
     3301 acgatgtccg gaccgctgca tattggtctg attcccacgg ttggaccgta cctgctaccg
     3361 catattatcc cgatgctgca ccagaccttt ccaaagctgg aaatgtatct gcatgaagca
     3421 cagacccacc agttactggc gcaactggac agcggcaaac tcgattgcgt gatcctcgcg
     3481 ctggtgaaag agagcgaagc attcattgaa gtgccgttgt ttgatgagcc aatgttgctg
     3541 gctatctatg aagaccatcc gtgggcgaac cgcgaatgcg taccgatggc cgatctggca
     3601 ggggaaaaac tgctgatgct ggaagatggt cactgtttgc gcgatcaggc aatgggtttc
     3661 tgttttgaag ccggggcgga tgaagataca cacttccgcg cgaccagcct ggaaactctg
     3721 cgcaacatgg tggcggcagg tagcgggatc actttactgc ctgcgctggc tgtgccgccg
     3781 gaacgcaaac gcgatggggt tgtttatctg ccgtgcatta agccggaacc acgccgcact
     3841 attggcctgg tttatcgtcc tggctcaccg ctgcgcagcc gctatgagca gctggcagag
     3901 gccatccgcg caagaatgga tggccatttc gataaagtgt taaaacaggc ggtttaagcc
     3961 atttagcgca gctacccgat aggcttccgc catcgtcggg tagttaaagg tggtgttgac
     4021 gaagtactca atagtgttgc cgccaccttt ctgttccata atcgcctgac cgatatgaat
     4081 aatttcggca gcgcgctcgc caaagcagtg aatacccaga atctcttttg tttcccgatg
     4141 gaacaaaatt ttcagcgtgc ccacgttcat gccgacgatt tgtgcacgtg ccagatgttt
     4201 aaactgggcg cggcctactt catatggcac tttcatcgcg gtcagctgct gttcggtttt
     4261 gcctacagag ctgatttccg ggatggtgta aatgccggta gggatatctt caatcagatg
     4321 tgcgttggct tcgcctttca ccagcgcctg cgcggcaatg cgcccctggt cataggctgc
     4381 cgacgccagg ctcggataac caatcacgtc gcccaccgcg taaacgtgcg gctgtgcggt
     4441 ctgatacatg ctgttgacct tcagctgtcc gcgactgtca gtttccagcc caatgttctg
     4501 taacgccagc gaatcggtat taccggtgcg accgttggca tagagcagac agtcagcttt
     4561 cagtttttta cccgacttca gatgcatgat cacaccgtcg tcacagcctt cgatcttctc
     4621 gtactcttcg ttgtgacgaa tcactacgcc actgttccag aagtgatagg agagagaatc
     4681 tgacatctct tgatcgagaa atgccagcag gcgatcgcgg gtgttgatca gatccacttt
     4741 tacatccata ccgcggaaga tcgacgcata ttcacagccg atcactccag caccgtaaat
     4801 aagtacatgg cgcggttcgt ggtgcatgct aagaattgag tcgctgtcat aaatgcgtgg
     4861 atgggtgaaa tcaacatctg ttggatgata tggacgagaa ccgcaggcaa taacaaattt
     4921 ttcagcagtt agtgtttcga cgctgccgtc cgggcaatcc agcgccaacg tatgctcatc
     4981 aacaaagcga gcgttcccct gcaatatttc acagtgatta cgttcgtaaa atccctgacg
     5041 catgcgcgtt tgttgattaa tcacgttatc ggcatggtta aggatatcgg caaaagaaga
     5101 gcggagcagt cgggaatggt cgctgtatag tgggttttga ttgaattcta taatgcggct
     5161 gacggcgtga cggagagctt tcgacgggat ggtgccccag tgggtgcaac cgccgccaac
     5221 attttgataa cgctcgataa ctgcgacgcg cgcaccttgc ttaaccaggc ccattgcagc
     5281 gccttcgccg ccggggccgg aacctattac tatggcatcg taatcgtagg aatgtggcat
     5341 ggtagggctt acctgttctt atacataaaa gcaacagaat ggtaacattt tatcgcgggt
     5401 aagccaattg atccccgtca tttatctggc tatatcgtga gcggcctttg ctttgtcttc
     5461 ctccttctta ttttatttat tcactattaa tcgctttttc ttatg
//
LOCUS       DADSYM010000200         5439 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2415
            SAMN08039579-rid11723173.denovo.202, whole genome shotgun sequence.
ACCESSION   DADSYM010000200 DADSYM010000000
VERSION     DADSYM010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039579
            Sequence Read Archive: SRR6362533
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5439)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5439)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980162.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 03:39:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,484
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 4,347
            CDSs (with protein)               :: 4,347
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 43 of 85
            Pseudo Genes (incomplete)         :: 37 of 85
            Pseudo Genes (internal stop)      :: 19 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5439
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039579-rid11723173.denovo.202"
                     /strain="LGE2415"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8302 N 95.9628 W"
                     /collection_date="2015-08-24"
     gene            complement(118..1401)
                     /gene="aroA"
                     /locus_tag="J1Y83_004054"
     CDS             complement(118..1401)
                     /gene="aroA"
                     /locus_tag="J1Y83_004054"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001764391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HBA7632147.1"
                     /translation="MESLTLQPIARVDGTINLPGSKSVSNRALLLAALAHGKTVLTNL
                     LDSDDVRHMLNALTALGVSYTLSADRTRCEIIGNGGPLHAESARELFLGNAGTAMRPL
                     AAALCLGSNDIVLTGEPRMKERPIGHLVDALRQGGAKITYLEQENYPPLRLQGGFTGG
                     NVDVDGSVSSQFLTALLMTAPLAPEDTVIRIKGDLVSKPYIDITLNLMKTFGVEIENQ
                     HYQQFVVKGGQSYQSPGTYLVEGDASSASYFLAAAAIRGGTVKVTGIGRNSMQGDIRF
                     ADVLEKMGATICWGDDYISCTRGELNAIDMDMNHIPDAAMTIATAALFAKGTTTLRNI
                     YNWRVKETDRLFAMATELRKVGAEVEEGHDFIRITPPEKLKFAEIATYNDHRMAMCFS
                     LVALSDTPVTILDPKCTAKTFPDYFEQLARISQPG"
     gene            complement(1472..2560)
                     /gene="serC"
                     /locus_tag="J1Y83_004055"
     CDS             complement(1472..2560)
                     /gene="serC"
                     /locus_tag="J1Y83_004055"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000057138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoserine/phosphohydroxythreonine
                     transaminase"
                     /protein_id="HBA7632148.1"
                     /translation="MAQIFNFSSGPAMLPAEVLKQAQQELRDWNGLGTSVMEVSHRGK
                     EFIQVAEEAEKDFRDLLNVPSNYKVLFCHGGGRGQFAAVPLNILGDKTTADYVDAGYW
                     AASAIKEAKKYCTPNVFDAKVTVDGLRAVKPMSEWQLSDNAAYMHYCPNETIDGIAID
                     ETPDFGKDVVVAADFSSTILSRPIDVSRYGVIYAGAQKNIGPAGLTIVIVREDLLGKA
                     NIACPSILDYSILNDNDSMFNTPPTFAWYLSGLVFKWLKANGGVAAMDKINQQKAELL
                     YGVIDNSDFYRNDVTKANRSRMNVPFQLADSALDKLFLEESFAAGLHALKGHRVVGGM
                     RASIYNAMPLEGVKALTDFMVEFERRHG"
     gene            complement(2759..3451)
                     /locus_tag="J1Y83_004056"
     CDS             complement(2759..3451)
                     /locus_tag="J1Y83_004056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HBA7632149.1"
                     /translation="MKAFDLHRMAFDKVPFDFLGEVALRSLYTFVLVFLFLKMTGRRG
                     VRQMSLFEVLIILTLGSAAGDVAFYDDVPMVPVLIVFITLALLYRLVMWLMAHSEKLE
                     DLLEGKPVVIIEDGELAWSKLNNSNMTEFEFFMELRLRGVEQLGQVRLAILETNGQIS
                     VYFFEDDKVKPGLLILPSDCTQRYKVVPESADYACIRCSEIIHMNAGEKQLCPRCANP
                     EWTKASRAKRVT"
     gene            3581..5341
                     /locus_tag="J1Y83_004057"
     CDS             3581..5341
                     /locus_tag="J1Y83_004057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415425.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     accessory protein YcaO"
                     /protein_id="HBA7632150.1"
                     /translation="MTQTFIPGKDAALEDSIARFQQKLSDLGFQIEEASWLNPVPNVW
                     SVHIRDKECALCFTNGKGATKKAALASALGEYFERLSTNYFFADFWLGETIANGPFVH
                     YPNEKWFPLTENDDVPEGLLDDRLRAFYDPENELTGSMLIDLQSGNEDRGICGLPFTR
                     QSDNQTIYIPMNIIGNLYVSNGMSAGNTRNEARVQGLSEVFERYVKNRIIAESISLPE
                     IPADVLARYPAVVEAIETLEAEDFPIFAYDGSLGGQYPVICVVLFNPANGTCFASFGA
                     HPDFGVALERTVTELLQGRGLKDLDVFTPPTFDDEEVAEHTNLETHFIDSSGLISWDL
                     FKQDADYPFVDWSFSGTTEEEFATLMAIFKKEDKEVYIADYEHLGVYACRIIVPGMSD
                     IYPAEDLWLANNSMGSHLRETILSLPGSEWEKEDYLNLIEQLDEEGFDDFTRVRELLG
                     LATGSDNGWYTLRIGELKAMLALAGGDLEQALVWTEWTMEFNSSVFSPERANYYRCMQ
                     TLLLLAQEEDRQPLQYLNAFVRMYGADAVEAASAAMSGEAAFYGLQPVDSDLHAFAAH
                     QSLLKAYEKLQRAKAAFWAK"
BASE COUNT         1414 a         1385 c         1270 g         1370 t
ORIGIN      
        1 attgataatt aacgctacca ttaataaaaa taccccacaa ggtccgaaaa aaaacgccgg
       61 caatgtgccg acgtctttga aaagaaagga ttgtctatgt tatcgcccgt tattcattca
      121 gccgggttga cttatccgcg ccaattgctc gaaatagtct ggaaaagttt tagcggtaca
      181 ttttggatca agaatcgtca caggcgtatc tgataatgcg accagcgaga aacacatcgc
      241 catccggtga tcattataag tggcgatctc agcaaatttc agtttttccg gtggcgtaat
      301 gcgaatgaaa tcatgcccct cttcgacttc cgcaccgact ttacgcagtt ctgttgccat
      361 cgcaaacagg cgatcggtct ctttaacacg ccagttatag atattgcgca gcgtggtggt
      421 gccttttgca aataacgccg cggtggcaat ggtcatcgcc gcatcgggaa tatggttcat
      481 atccatatca atggcgttca gttcaccacg cgtgcaggaa atataatcat cgccccagca
      541 aatggtcgcg cccatttttt ccagaacatc agcaaagcga atatcaccct gcatactgtt
      601 gcgtccaatg ccggtcactt ttacagtgcc gcctctgatt gctgctgctg ccaggaaata
      661 agaagccgaa gatgcatcgc cttcaaccaa ataagtgccc ggagactggt aagactgccc
      721 cccttttacg acaaattgtt gatagtgctg attttcaatt tcaacaccaa acgtcttcat
      781 cagattgagt gtgatgtcga tataaggttt agaaaccaga tcgcctttaa tacgaatcac
      841 cgtatcttcc ggcgcaagag gtgcagtcat taacagtgcg gtgaggaact ggcttgaaac
      901 ggagccatca acatcaacgt tgccgccagt aaagccgccc tgtaaacgca atggcggata
      961 attttcttgt tccaggtaag tgatcttcgc cccgccctgg cgcagcgcat ccaccagatg
     1021 accaatcggg cgttctttca ttcgcggttc accggtcagt acaatatcat tgctacccag
     1081 gcaaagtgct gccgccagcg gacgcattgc cgttccggcg ttaccgagga acaactcacg
     1141 ggcactttct gcgtgtaatg gaccaccgtt accgataatt tcgcaacgcg tacgatcggc
     1201 tgaaagcgta tagcttaccc ctaacgctgt taatgcattc agcatatggc gcacgtcatc
     1261 gctatccagc agattggtta atactgtttt gccgtgtgct aatgccgcca gcaataaagc
     1321 gcggttagaa acgctcttgg aaccgggcag attaatagtg ccatcgacac gagcgatggg
     1381 ttgtaacgtt agggattcca tgaactcaac tctctacaac agaaataaaa accccacagg
     1441 ctggctgtgg ggattatgca aaatttcgac attagccgtg acgacgttcg aactcaacca
     1501 tgaagtctgt cagtgcttta acaccttcca gcggcatggc gttataaata gacgcacgca
     1561 ttccgccgac cactcggtga cctttcagcg catgtaagcc cgcagcgaaa gactcttcga
     1621 ggaacaattt atcaagcgcg ctatccgcca actggaacgg cacgttcatc cgcgaacggt
     1681 tagctttcgt cacatcattg cggtagaaat cgctgttatc aatcacccca tacagcagtt
     1741 ctgctttttg ctgattgatc ttatccattg cagccacacc gccgttcgct ttcagccatt
     1801 tgaagaccag gccagataag taccaggcaa atgtcggcgg cgtgttgaac atggaatcgt
     1861 tatcgttgag gatggagtaa tccagaatcg atgggcaggc gatattcgct ttgcccagca
     1921 aatcttcacg aacgatgacg attgtcaggc cagccgggcc gatatttttc tgcgcgccag
     1981 cgtaaattac accataacgg ctgacatcaa tcggacggga aagaatggtt gaagagaagt
     2041 cagcggcgac caccacgtct ttaccgaagt ctggtgtttc atcgatagcg ataccgtcaa
     2101 tggtttcgtt cgggcagtaa tgcatataag cagcattatc agagagttgc cattcactca
     2161 ttggcttaac tgcgcgaaga ccatcaacgg tcactttcgc atcaaacaca tttggtgtgc
     2221 agtatttttt cgcttctttg atagcacttg ctgcccagta acccgcatca acataatctg
     2281 cagtggtttt atcaccaaga atattcagcg gtacagcagc aaattgaccg cgaccaccgc
     2341 catggcagaa caataccttg tagttggagg ggacgttaag aagatcgcga aaatccttct
     2401 cggcttcctc tgcaacctga atgaactctt tgccacggtg actcacttcc atcaccgacg
     2461 taccaagacc gttccagtcg cgcagttcct gttgagcctg tttaagcacc tctgccggta
     2521 gcattgccgg accagaacta aaattgaaga tttgagccat ttcccctcac cacgttgcgt
     2581 tgccattaac ccgcggtcaa tcaattgcga ccacaggcaa tacattattc ctgtggctat
     2641 cggttttatc attcagtgac acgcatcgca atgagtaaaa ccgttgaggg caaaaatgag
     2701 gcacgcgtca cacaaaagaa tctctcggat tattttcaaa aaaccgatgt tttggctgtc
     2761 aggtcacccg tttagcccga cttgccttcg tccattctgg atttgcacag cgcggacata
     2821 attgtttttc ccccgcgttc atatgaatga tttcactgca acggatgcag gcatagtccg
     2881 ccgactccgg caccactttg taacgctgag tacagtcact gggtaaaata agtaatcccg
     2941 gtttcacctt gtcatcttca aagaaataaa cactgatttg cccgttggtt tcgagaatcg
     3001 ccagacgtac ctgccccaac tgctctacac cgcgcaatcg tagctccata aagaactcaa
     3061 attccgtcat gttggagtta ttgagtttcg accaggccag ctcgccatct tcaataatga
     3121 caaccggctt gccttccaga agatcttcca gtttttcact gtgcgccatc aaccacatta
     3181 ccaggcggta taacaacgcc agagtaataa agacgataag taccgggacc atcggcacat
     3241 catcataaaa cgccacatct cccgccgctg aacccagtgt cagaatgatt aacacttcaa
     3301 acagcgacat ctgccgcaca ccgcgtcttc cggtcatttt gaggaacaaa aagaccagta
     3361 caaaggtata aagactacgt agtgcaactt cgccaaggaa atcaaaaggc actttatcga
     3421 atgccatacg gtggagatca aacgccttca tttttatgct cttatcgtag ttaatccgct
     3481 aaaagcatag ctaacagttc atcagcgtgg cggcggtgat agcacctttg agataaaacc
     3541 cgtattattg cgcgctttcc gtacgactaa agtgattttc atgacgcaaa catttatccc
     3601 cggcaaagat gccgctctgg aagattccat cgcacgcttc cagcaaaaac tttcagacct
     3661 cggttttcag attgaagagg cctcctggct gaatcccgtg cctaatgtct ggtctgtaca
     3721 tattcgcgac aaagagtgcg cactgtgttt taccaacggt aaaggcgcaa ccaaaaaagc
     3781 ggcgctggct tctgccctcg gtgaatattt cgagcgtctc tcaaccaact acttttttgc
     3841 ggacttctgg ctgggcgaaa ccatcgccaa cggcccattc gtgcattatc ccaacgaaaa
     3901 atggttccca ctgaccgaaa atgacgatgt accagaaggg ctgctcgatg accgtctgcg
     3961 cgcattttac gatccggaga atgaactgac cggtagtatg ctgattgacc tgcaatccgg
     4021 taacgaagat cgtggtattt gcggtctacc gtttacgcgc cagtccgaca atcagaccat
     4081 ttatattccg atgaatatca ttggtaactt gtacgtttct aacggtatgt ctgctggcaa
     4141 tacccgtaac gaagcacgcg ttcagggatt gtctgaagtt ttcgaacgtt acgtgaaaaa
     4201 ccgcattatt gctgaaagca tcagcttgcc ggaaatcccg gcagacgtgc tggcacgtta
     4261 tccagcagta gttgaagcga tcgaaacgct ggaagcggaa gatttcccga tcttcgctta
     4321 tgacggttcg cttggcggcc agtatccggt gatttgcgtg gtactgttca atcctgctaa
     4381 cggtacctgc tttgcctcat tcggtgcgca tcctgatttt ggcgttgcac tggaacgtac
     4441 cgtgaccgag ctgctgcaag gtcgtggcct aaaagatctg gatgtgttta ctccaccaac
     4501 cttcgatgat gaagaagtcg ctgaacatac caacctcgaa acgcacttta tcgattccag
     4561 cggtttaatc tcctgggacc tgttcaagca ggatgccgat tatccgtttg ttgactggag
     4621 cttctccggc accacggaag aagagttcgc cacgctgatg gctatcttca aaaaggaaga
     4681 taaagaagtt tatattgccg attacgagca tctgggtgtc tacgcttgcc gtattatcgt
     4741 gcctggcatg tccgatattt atccggctga agatctgtgg ctggcgaata acagtatggg
     4801 cagccattta cgtgaaacga ttctttcgct accaggtagc gagtgggaaa aggaagatta
     4861 cctgaacctc atcgagcaac tggatgaaga aggttttgat gactttaccc gcgtgcgtga
     4921 actgttgggt ctggcgaccg ggtcggataa cggctggtac actctgcgta tcggtgaatt
     4981 aaaagccatg ctggcgctgg ctggtggcga tctggaacag gctctggtct ggaccgaatg
     5041 gacgatggag tttaactcat cagtattcag cccggaacgc gccaattatt atcgctgcat
     5101 gcaaacgttg ttattactgg cgcaggaaga agatcgccag ccgctgcaat atctgaatgc
     5161 gtttgttcgc atgtacggcg cagatgccgt ggaagccgcc agtgcggcaa tgagcggcga
     5221 agcggcattt tatggcttgc aaccagtaga tagcgatctg cacgcgtttg ctgcacatca
     5281 gtcgttgttg aaggcctacg aaaagctgca acgcgccaaa gcagcattct gggcaaaata
     5341 aaatcaaata gcctacgcaa tgtaggctta atgattcgtc tgagttatat tacggggcgt
     5401 ttttttaatg ccccgcttta catatatttg cattaataa
//
LOCUS       DADSYM010000201         5425 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2415
            SAMN08039579-rid11723173.denovo.203, whole genome shotgun sequence.
ACCESSION   DADSYM010000201 DADSYM010000000
VERSION     DADSYM010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039579
            Sequence Read Archive: SRR6362533
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5425)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5425)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980162.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 03:39:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,484
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 4,347
            CDSs (with protein)               :: 4,347
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 43 of 85
            Pseudo Genes (incomplete)         :: 37 of 85
            Pseudo Genes (internal stop)      :: 19 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5425
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039579-rid11723173.denovo.203"
                     /strain="LGE2415"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8302 N 95.9628 W"
                     /collection_date="2015-08-24"
     gene            136..1416
                     /gene="wcaK"
                     /locus_tag="J1Y83_004058"
     CDS             136..1416
                     /gene="wcaK"
                     /locus_tag="J1Y83_004058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis pyruvyl transferase
                     WcaK"
                     /protein_id="HBA7632151.1"
                     /translation="MKLLILGNHTCGNRGDSAILRGLLDAINILNPHAEVDVMSRYPV
                     SSSWLLNRPVMGDPLFLQMKQHNSAAGVVGRVKKVLRRRYQHQVLLSRVTDTGKLRNI
                     AIAQGFTDFVRLLSGYDAIIQVGGSFFVDLYGVPQFEHALCTFMAKKPLFMIGHSVGP
                     FQDEQFNQLANYVFGHCDALILRESVSLDLMKRSNITTAKVEHGVDTAWLVDHHIEDF
                     TASYAVQHWLDVAAQQKAVAITLRELAPFDKRLGTTQQAYEKAFAGVVNRILDEGYQV
                     IALSTCTGIDSYNKDDRMVALNLRQHISDPARYHVVMDELNDLEMGKILGACELTVGT
                     RLHSAIISMNFATPAIAINYEHKSAGIMQQLGLPEMAIDIRHLLDGSLQAMVADTLGQ
                     LPALNTRLNEAVSRERQTGMQMVQSVLERIGEVK"
     gene            1413..2633
                     /gene="wcaL"
                     /locus_tag="J1Y83_004059"
     CDS             1413..2633
                     /gene="wcaL"
                     /locus_tag="J1Y83_004059"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaL"
                     /protein_id="HBA7632152.1"
                     /translation="MKVGFFLLKFPLSSETFVLNQITAFIDMGFEVEIIALQKGDTQN
                     THAAWTKYNLAARTRWLQDEPTGKVAKLRHRASQTLRGIHRKNTWQALNLKRYGAESR
                     NLILSAICGQVATPFHADVFIAHFGPAGVTAAKLRELGVIRGKIATIFHGIDISSREV
                     LNHYTPEYQQLFRRGDLMLPISDLWAGRLQKMGCPREKIAVSRMGVDMTRFSPRPVKA
                     PATPLEIISVARLTEKKGLHVAIEACRQLKEQGVAFRYRILGIGPWERRLRTLIEQYQ
                     LEDVVEMPGFKPSHEVKEMLDDADVFLLPSVTGADGDMEGIPVALMEAMAVGIPVVST
                     LHSGIPELVEADKSGWLVPENDARALAQRLAAFSQLDSDELAPVVKRAREKVEHDFNQ
                     QVINRELASLLQAL"
     gene            2644..4038
                     /gene="wcaM"
                     /locus_tag="J1Y83_004060"
     CDS             2644..4038
                     /gene="wcaM"
                     /locus_tag="J1Y83_004060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis protein WcaM"
                     /protein_id="HBA7632153.1"
                     /translation="MPFKKLSRRTFLTASSALAFLHTPFARALPARQSVNINDYNPHD
                     WIASFKQAFSEGQTVVVPAGLVCDNINTGIFIPSGKTLHLLGSLRGNGRGRFVLQDGS
                     QVTGEGGGSMHNITLDVRGSDCTIKGLVMSGFGPVTQIYIGGKNKRVMHNLTIDNLTV
                     SHANYAILRQGFHNQIIGANITNCKFSDLQGDAIEWNVAINDRDILISDHLIERINCT
                     NGKINWGIGIGLAGSTYDNNYPENQAVKNFVVANITGSDCRQLIHVENGKHFVIRNIK
                     ARNITPDFSKKAGIDNATVAIYGCDNFVIDNIEMINSAGMLIGYGVIKGKYLSIPQNF
                     RVNNIQLDNTHLAYKLRGIQISAGNAVSFVALTNIAMKRASLELHNKPQHLFMRNINV
                     MQESSVGPALSMNFDMRKDVRGVFMAKKETLLSLANVHAVNEKGQSSVDIDRINHHIV
                     NVEKINFRLLERRE"
     gene            4213..5106
                     /gene="galF"
                     /locus_tag="J1Y83_004061"
     CDS             4213..5106
                     /gene="galF"
                     /locus_tag="J1Y83_004061"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalF"
                     /protein_id="HBA7632154.1"
                     /translation="MTNLKAVIPVAGLGMHMLPATKAIPKEMLPIVDKPMIQYIVDEI
                     VAAGIKEILLVTHASKNAVENHFDTSYELESLLEQRVKRQLLAEVQSICPPGVTIMNV
                     RQGEPLGLGHSILCARPAIGDNPFVVVLPDVVIDDASADPLRYNLAAMIARFNETGRS
                     QVLAKRMPGDLSEYSVIQTKEPLDREGKVSRIVEFIEKPDQPQTLDSDIMAVGRYVLS
                     ADIWPELERTQPGAWGRIQLTDAIAELAKKQSVDAMLMTGDSYDCGKKMGYMQAFVKY
                     GLRNLKEGAKFRKGIEKLLSE"
BASE COUNT         1412 a         1358 c         1396 g         1259 t
ORIGIN      
        1 aacgccttat ccgtcctacg gttctgtgct cggatcttgt aggcctgata agacgcgtta
       61 gcgtcgcatc aggcaaaaaa cggcaacgaa tacaacaaac acagcctcac ctggcatcaa
      121 atttatgagg aaaaaatgaa attacttatt ctgggcaacc acacttgcgg caatcgtggc
      181 gatagcgcca tcctgcgtgg cttacttgat gccatcaata tcctcaatcc acatgccgaa
      241 gtggacgtga tgagccgcta tccggtcagt tcttcctggc tgctcaaccg cccggtaatg
      301 ggcgatccgc tgttcctgca aatgaaacaa cacaacagcg cggcgggcgt tgtcgggcgc
      361 gttaaaaaag tcctccgtcg ccgctaccag caccaggtat tgctctcacg cgtcaccgac
      421 actggcaagc tgcgtaatat cgccatcgcc cagggattca ctgacttcgt gcgactgctg
      481 tcaggttacg acgccattat tcaggtcggc ggatcgtttt ttgtcgatct ctacggcgtg
      541 ccgcagtttg aacatgcgct ttgcactttc atggcgaaaa agccgctgtt tatgattggt
      601 cacagcgtcg gccccttcca ggatgagcaa tttaaccaac tggcgaacta tgtctttggt
      661 cactgcgacg cgctgatcct gcgcgaatcg gtcagccttg atttgatgaa acgcagcaat
      721 atcaccactg caaaagttga acatggcgtc gataccgcgt ggctggtcga tcaccacatt
      781 gaagacttca ccgccagcta tgccgttcaa cactggctgg atgttgccgc acaacagaaa
      841 gcggtggcaa ttaccctgcg cgaactggca ccgttcgaca aacgtctcgg caccacccaa
      901 caagcgtatg aaaaagcctt tgccggggtg gtcaatcgca ttctcgatga agggtatcag
      961 gtgattgcgc tctccacctg tacgggtatc gacagctata acaaagacga ccgcatggtg
     1021 gcgctcaatc tgcgccagca catcagcgat cctgcccgtt accacgtggt gatggatgaa
     1081 ctcaacgatc tggaaatggg caaaattctg ggggcctgtg aactcaccgt cggtacgcgc
     1141 ctgcactctg ccattatctc gatgaacttt gccactccgg cgattgccat caactacgaa
     1201 cataaatccg ccgggatcat gcaacaactg gggctaccag aaatggcgat tgatatccgt
     1261 catttattag acggcagcct gcaagcgatg gttgcggata ccttaggcca gcttccggca
     1321 ctgaacacgc gacttaacga agccgtcagt cgcgaacgtc agacgggaat gcagatggtg
     1381 caatctgtgc ttgaacgcat cggggaggtg aaatgaaggt cggcttcttt ttactgaaat
     1441 ttccgctgtc gtcggaaacc ttcgttctta accaaatcac tgcgtttatt gatatgggat
     1501 ttgaggtgga gattatcgcg ctgcaaaaag gcgacacaca aaacacccac gcggcatgga
     1561 cgaaatacaa ccttgctgcc agaacccgct ggttacagga cgaacctacg ggcaaagtgg
     1621 cgaaactgcg ccaccgcgcc agccagacct tacgcggcat tcatcgtaaa aatacctggc
     1681 aggcgctcaa cctcaaacgc tatggtgccg agtcgcggaa cctgattttg tctgccattt
     1741 gtggtcaggt cgcaacaccg tttcatgccg atgtctttat cgctcacttt ggtccggcag
     1801 gggtaaccgc agcaaaactc cgcgaactgg gtgtcattcg cggcaaaatt gccaccattt
     1861 tccacggcat tgatatttcc agtcgggaag tgctcaacca ctacactcct gaatatcagc
     1921 aactgttccg ccgtggcgac ctgatgctac cgatcagcga tctgtgggcc ggaaggctgc
     1981 aaaaaatggg ctgcccgagg gaaaaaattg ccgtatcgcg catgggtgta gacatgacgc
     2041 gctttagccc acgtccggtg aaagcgcccg caacaccgct ggaaatcatt tccgtcgcac
     2101 gcttaaccga gaaaaaaggc ctgcatgtgg cgattgaagc ctgccgtcag ttgaaagagc
     2161 agggcgtggc atttcgctat cgcatcctcg gcattggccc gtgggaacga cgcctgcgca
     2221 cgctcatcga acaatatcaa ctggaagatg tggtagagat gccgggcttt aaaccgagcc
     2281 acgaagtgaa agagatgctc gatgacgcgg atgtcttcct gttgccatcg gttacaggtg
     2341 cggatggtga tatggaaggt attccggtgg cgctaatgga agcgatggcg gtcggcattc
     2401 cggttgtttc tactctgcat agcggaatac cggaactggt ggaggctgac aaatccggct
     2461 ggctggtgcc tgagaacgat gctcgcgcac tggcgcaacg actggcggcg tttagccaac
     2521 tggacagcga cgaactggct cctgtcgtca aacgtgcgcg cgaaaaagtc gaacacgatt
     2581 ttaaccagca ggtgattaat cgagaactcg ccagcttgct gcaggcttta tagaggagaa
     2641 cgcatgccat ttaaaaaact ctcccgacgc accttcctga cggcaagctc cgcgcttgcc
     2701 ttcctccata cccctttcgc ccgtgcgctt cccgcccgac aaagcgttaa cattaacgac
     2761 tacaaccctc acgactggat cgcctcattt aaacaagcct tcagcgaagg gcaaacggtc
     2821 gtcgtgcctg ccggattggt ttgtgacaat atcaacaccg gcatcttcat tccttctggc
     2881 aaaacgttac acctccttgg aagcctgcgc ggcaacggca gagggcgatt tgtcttacag
     2941 gatggcagcc aggtgacagg ggaggggggc ggcagtatgc ataacatcac cctggatgtg
     3001 cgcggctctg actgcaccat caaagggctg gtgatgagcg gctttggccc ggtgacgcag
     3061 atctatatcg gcggcaaaaa caaacgggtc atgcacaacc tgaccatcga taacctgacc
     3121 gttagccacg ctaattacgc catcttacgc cagggatttc ataaccagat tatcggtgcc
     3181 aacatcacca attgtaagtt cagcgactta caaggcgacg ccattgaatg gaacgtggca
     3241 attaacgacc gcgatatttt gatctccgac catctcatcg agcgcatcaa ctgtaccaac
     3301 ggcaaaatca actggggcat cggcataggc cttgcgggaa gtacctacga taataattat
     3361 ccggaaaacc aggcagtgaa aaactttgtc gtggcgaata tcacggggtc ggattgtcga
     3421 cagttgatcc atgttgaaaa tggtaaacat tttgttattc gtaatatcaa agcccgcaat
     3481 atcacaccgg atttcagtaa gaaagcaggt attgataacg ccacggtcgc tatttacggt
     3541 tgtgacaatt tcgtgattga taatattgaa atgattaata gtgccgggat gttaatcggt
     3601 tatggggtaa ttaaaggcaa atatctctcg ataccgcaga atttccgggt gaataatatt
     3661 caactggata acacccacct tgcttataaa ttgcgcggca tccaaatctc tgccgggaat
     3721 gctgtctcct ttgtggcact gactaacatt gcgatgaagc gtgcgtcatt ggagctgcac
     3781 aacaagccgc aacacctttt tatgcgcaat atcaatgtga tgcaggaatc ctcagttgga
     3841 cccgcattga gcatgaactt tgacatgcgc aaagacgttc gaggcgtgtt tatggcgaaa
     3901 aaagaaacac tgctgtctct tgcaaatgtt catgcggtga atgaaaaagg gcaaagctcc
     3961 gtcgatattg acaggattaa tcaccatatt gttaatgtgg aaaagattaa ctttagattg
     4021 ctggaacgga gagagtagat ttgcgaccat tcctggaaaa atggagtcat acttaggaac
     4081 aatgctactg caatccacaa cgaagcggcg taacatcaca agtaattcag taatcaattc
     4141 agggtaattg atgctggcga aaaaaatcga acaagctata attcagcaac cattttacag
     4201 gtggatgaaa taatgacgaa tttaaaagca gttattcctg tagcgggtct agggatgcat
     4261 atgttgcctg ccactaaggc gattcccaaa gagatgctac cgatcgtcga caagccaatg
     4321 attcagtaca ttgttgacga gattgtggct gcagggatca aagaaatcct cctggtaact
     4381 cacgcgtcca agaacgcggt cgaaaaccac ttcgacacct cttatgaatt agaatctctc
     4441 cttgaacagc gcgtgaagcg tcaactgctg gcggaagtgc aatctatctg cccgccgggc
     4501 gtcactatta tgaacgtgcg tcagggcgaa cctttaggtt taggccactc cattttgtgt
     4561 gcgcgacctg ccattggtga caacccgttt gtcgtggtgc tgccagacgt tgtgatcgac
     4621 gacgccagcg ccgacccgct gcgctacaac cttgctgcca tgattgcgcg cttcaacgaa
     4681 acgggccgca gccaggtgct ggcaaaacgt atgccgggtg acctctctga atactctgtc
     4741 atccagacca aagaaccact ggatcgtgaa ggtaaagtca gccgcattgt tgaatttatc
     4801 gaaaaaccgg atcagccgca gacgctggac tccgacatca tggccgttgg tcgctatgtg
     4861 ctttctgccg atatttggcc ggaacttgaa cgcactcagc ctggtgcatg ggggcgtatt
     4921 cagctgactg atgccattgc cgaactggcg aaaaaacagt ccgttgatgc catgctgatg
     4981 accggtgaca gttacgactg cggcaaaaaa atgggttata tgcaggcgtt tgtgaagtat
     5041 ggactgcgta acctaaaaga aggggcgaag ttccgtaaag ggattgagaa gctgttaagc
     5101 gaataatgaa tatctgaccg gatgtaacgg ttgataagaa aattataacg gcagtgaaga
     5161 ttcgtggcga aagtaatttg ttgcgaattt tcctgccgtt gttttatata aacaatcaga
     5221 ataacaacga gttagcaata ggattttcgt caaagttttc caggattttc cttgtttcca
     5281 gagcggattg gtaagacaat tagcgtttga atttttcggg tttagcgcga gtgggtaacg
     5341 ctcgccacat cgtaggcatg catgcagtgc tctggtagct gtaaagccag gggcggtagc
     5401 gtgtgtaatt aaaaataaaa ctgtg
//
LOCUS       DADSYM010000202         5392 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2415
            SAMN08039579-rid11723173.denovo.204, whole genome shotgun sequence.
ACCESSION   DADSYM010000202 DADSYM010000000
VERSION     DADSYM010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039579
            Sequence Read Archive: SRR6362533
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5392)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5392)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980162.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 03:39:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,484
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 4,347
            CDSs (with protein)               :: 4,347
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 43 of 85
            Pseudo Genes (incomplete)         :: 37 of 85
            Pseudo Genes (internal stop)      :: 19 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5392
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039579-rid11723173.denovo.204"
                     /strain="LGE2415"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8302 N 95.9628 W"
                     /collection_date="2015-08-24"
     gene            214..1434
                     /gene="arcA"
                     /locus_tag="J1Y83_004062"
     CDS             214..1434
                     /gene="arcA"
                     /locus_tag="J1Y83_004062"
                     /EC_number="3.5.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_463327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase"
                     /protein_id="HBA7632155.1"
                     /translation="MEKHYVGSEIGQLRSVMLHRPNLSLKRLTPSNCQELLFDDVLSV
                     ERAGEEHDIFANTLRQQGIEVLLLTDLLTQTLDIPEAKSWLLETQISDYRLGPTFATD
                     VRTWLAEMSHRDLARHLSGGLTYSEIPASIKNMVVDTHDINDFIMKPLPNHLFTRDTS
                     CWIYNGVSINPMAKPARQRETNNLRAIYRWHPQFAGGEFIKYFGDENINYDHATLEGG
                     DVLVIGRGAVLIGMSERTTPQGIEFLAQALFKHRQAERVIAVELPKHRSCMHLDTVMT
                     HIDIDTFSVYPEVVRPDVNCWTLTPDGHGGLKRTQESTLLHAIEKALGIDQVRLITTG
                     GDAFEAEREQWNDANNVLTLRPGVVVGYERNIWTNEKYDKAGITVLPIPGDELGRGRG
                     GARCMSCPLHRDGI"
     gene            1445..2377
                     /gene="arcC"
                     /locus_tag="J1Y83_004063"
     CDS             1445..2377
                     /gene="arcC"
                     /locus_tag="J1Y83_004063"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006686402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HBA7632156.1"
                     /translation="MENKPTLVIALGGNALLKRGEPLEAEIQRKNIDLAAKTIAQLTQ
                     HWRVVLVHGNGPQVGLLALQNSAYAHVAPYPLDILGAESQGMIGYMLQQALKNQLLQR
                     EISVLLTQVEVDANDPAFSNPTKYIGPIYDHAQTQVLQAEKGWVFKADGHSFRRVVPS
                     PQPKRIVERDAIQTLIAHDHLVICNGGGGVPVVEKADGYHGIEAVIDKDLSAALLASQ
                     IHADALLILTDADAVYLDWGKPTQRPLAQVTPELLNEMQFDAGSMGPKVTACAKFVSQ
                     CRGIAGIGSLADGPEILAGDKGTLIRLDTPHNHA"
     gene            2401..3405
                     /gene="argF"
                     /locus_tag="J1Y83_004064"
     CDS             2401..3405
                     /gene="argF"
                     /locus_tag="J1Y83_004064"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_463325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HBA7632157.1"
                     /translation="MATSLKNRNFLKLLDYTPAEIQYLIDLAINLKAAKKSGNEKQTL
                     VGKNIALIFEKSSTRTRCAFEVAAFDQGAQVTYIGPSGSQIGHKESMKDTARVLGRMY
                     DGIEYRGYGQNIVEELGEFAGVPVWNGLTNEFHPTQILADLMTMLEHAPGKTLPELSF
                     AYLGDARNNMGNSLMVGAAKMGMDIRLVAPKSFWPDEALVTHCREIASVTGARITLTE
                     DVEEGVYDVDFLYTDVWVSMGEPKEAWAERVSLMAPYQINQQVITATRNPEVKFMHCL
                     PAFHNEHTTVGREIEMAYGLKGLEVTEEVFESAHSIVFDEAENRMHTIKAVMVATLGD
                     "
     gene            3462..4865
                     /locus_tag="J1Y83_004065"
     CDS             3462..4865
                     /locus_tag="J1Y83_004065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_463324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HBA7632158.1"
                     /translation="MGKFKFPTAYTILFVLIALVAAMTWIVPAGKYQMVTNAALGKEV
                     PVAGTYAPVEAQPQGITAVLLAPIDGLYNHQTYTAGAIDVALFVLIIGGFLGVVNKTG
                     AIDAGIERVTTRLNGREEWMIPILMALFAAGGTIYGMAEESLPFYTLLVPVMMAARFD
                     PLVAAATVLLGSGIGTLGSTINPFATVIAANAAGIPFTQGILLRVILLLVGYVICVYW
                     VMRYARKVRNSPESSIVADKMAENQAHFLGNRSETMLEFTPTRKAILILFAASFAFMI
                     YGVAVLGWWMAEISAVFLAAAVIVGVIARMGEETFTSTFIDGARDLLGVALIIGIARG
                     IVVVMDNGMITHTILHSAENLVSGLSTTVFINVTYWLEVLLSFLVPSSSGLAVLTMPI
                     LAPLADFAHVQRDLVVTAYQSASGVVNLITPTSAVVMGGLAIARVPWVRYLRWVAPLL
                     LILTMLNMIVLSIAAMI"
     gene            4981..>5392
                     /locus_tag="J1Y83_004066"
     CDS             4981..>5392
                     /locus_tag="J1Y83_004066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_463323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgR family transcriptional regulator"
                     /protein_id="HBA7632159.1"
                     /translation="MKEYDDYSAKEKKQLAVCQRLITEKSYLSQEEIRRDLQNHGFDS
                     ISQSTVSRLLKLLGVIKIRNTKGQKIYSVNPQLLPTPDAGRSVAEMVLSVEHNGEFIL
                     IHTVAGYGRAVARILDFHALPEILGVIAGSNIVWV"
BASE COUNT         1369 a         1290 c         1344 g         1389 t
ORIGIN      
        1 cgtattttgt ttctttatca tttatcagaa ccgcatcaaa tattatcaac ttcgattatt
       61 aatatcaccg ccggtgtgaa agtcgtcaca ttttctggaa gaaataaact gaatcattgc
      121 atctgaaaat tactgaacag acctgactac aagcatttaa tttgtttgca gccatctcct
      181 ctttattttt gaaaaagtgt taaaggaata atgatggaaa agcattatgt tggttctgaa
      241 attggacaac tacgcagcgt tatgttgcat cgcccaaatc tcagtctgaa acgtctaaca
      301 ccatccaatt gccaggagtt gttatttgac gatgtattgt ccgttgagcg ggcaggtgaa
      361 gagcatgata ttttcgccaa tacgctgcgc cagcaaggga tagaagttct gttactcact
      421 gatttactaa cacaaacatt ggatatcccg gaagccaaaa gctggttact ggaaacacaa
      481 atatcagatt accgactcgg ccctacgttt gctactgacg ttcgcacctg gcttgcagag
      541 atgtcacacc gggaccttgc tcgtcattta agtggtgggc tgacttatag tgaaattccc
      601 gcctcaatta aaaatatggt ggttgatacc cacgatatta atgatttcat tatgaagccg
      661 ttaccgaatc atttatttac gcgtgatacc tcatgctgga tttataacgg tgtatcgatt
      721 aatccaatgg caaaacctgc tcgccaacgt gaaaccaata atctacgcgc tatttatcgc
      781 tggcacccac aatttgctgg cggcgaattt attaaatatt tcggcgacga gaatattaat
      841 tatgaccacg ccacgttaga aggtggtgat gtgctggtga ttggtcgggg ggccgtgttg
      901 atcggcatgt ctgaacgcac cacgccacaa ggaatcgagt ttcttgctca ggcattgttt
      961 aaacatcgtc aggctgaacg ggtaatcgct gttgagctgc ctaaacaccg ctcctgtatg
     1021 catcttgaca ccgttatgac tcatatcgat atcgacacct tctcggttta tcccgaagtt
     1081 gtgcgcccgg atgttaattg ctggacgcta acacctgacg gtcacggcgg cctgaaacgt
     1141 actcaagaaa gcacactcct ccatgctatc gaaaaagccc tcggtattga ccaggtacgt
     1201 ttaatcacca cggggggtga tgcctttgag gcagaacgcg aacagtggaa tgacgctaac
     1261 aatgtgctca cgcttcggcc gggagtggta gtgggctacg agcgcaacat ctggactaac
     1321 gaaaaatatg acaaagcagg catcaccgtt ttacccatcc ccggtgatga attaggccgc
     1381 ggacgtggcg gcgcacgttg catgagttgc cctttgcatc gtgacggtat ctaaggaaga
     1441 gatcatggaa aacaaaccaa cactggttat cgctctcggc ggtaatgccc tgcttaaacg
     1501 aggcgaacca ctcgaagcag aaatccaacg taaaaatatc gatcttgcgg ctaaaactat
     1561 cgcccaactg actcaacact ggcgggttgt actggttcac gggaatggcc cacaagttgg
     1621 acttctggca cttcaaaata gcgcctatgc ccatgtcgct ccctatccac tcgacattct
     1681 gggggccgaa agccagggga tgatcggcta catgctacaa caggcgctga aaaatcaact
     1741 actacaacgt gaaatcagcg tgctgctgac gcaggttgaa gtggatgcca acgatcccgc
     1801 cttcagtaac ccaactaagt acatcggccc aatttatgac cacgctcaga ctcaggtact
     1861 acaggcagaa aaaggctggg ttttcaaagc agatggtcac tctttccgcc gggtcgtacc
     1921 ttctccacaa cctaaacgca tcgtcgagcg ggatgccatc cagacactga tcgcccacga
     1981 tcatctggtg atctgtaatg gtggcggtgg tgttccggtg gttgaaaaag ccgatggata
     2041 ccatgggatt gaagcggtta tcgacaaaga tctttcagcc gcgctactgg caagtcagat
     2101 ccatgctgac gccttgttga tcctcactga tgcggacgca gtatatctcg actggggcaa
     2161 gccaacccaa cgcccgctgg cccaggtcac gccggaatta ctcaacgaaa tgcagtttga
     2221 tgccgggtct atggggccga aagtcaccgc ctgcgcaaag tttgttagtc agtgccgggg
     2281 cattgcaggg atcggctctc ttgcagatgg tccggaaatt ctcgccggag acaaaggcac
     2341 tcttattcgc cttgataccc cccataacca cgcttgatcc atttttataa ggacatcccc
     2401 atggctactt cactgaaaaa ccgtaatttt cttaagctgc ttgattacac cccagcagag
     2461 attcaatacc tgattgattt ggctatcaat cttaaagccg ccaagaaatc tggaaacgaa
     2521 aaacaaacgc tggtcggtaa aaacattgcg ttgatttttg aaaaatcctc tacccgtacc
     2581 cgctgtgcct ttgaagtcgc ggcctttgat caaggtgcgc aggtgactta catcggcccc
     2641 agcggctcac aaatcggcca taaagaatca atgaaagata ccgctcgcgt actcggacgc
     2701 atgtatgacg gtattgagta tcgcggttat ggtcagaata tcgttgaaga attaggtgag
     2761 ttcgctggtg ttccggtatg gaacggactg accaatgaat ttcacccgac acagatcctc
     2821 gcggatttaa tgaccatgct ggaacatgcg ccaggcaaaa cgctgccaga gctgagcttt
     2881 gcgtatcttg gtgatgcccg aaataacatg gggaactcgc tgatggttgg tgctgcaaaa
     2941 atggggatgg atatccgtct ggtggcacca aaatctttct ggccagatga agcgctggta
     3001 acacactgcc gtgagatcgc cagcgtgact ggggcgagaa tcaccctgac agaggatgtg
     3061 gaagaaggcg tatacgacgt tgattttctc tatacggatg tttgggtttc gatgggagag
     3121 ccaaaagaag cctgggcaga acgagtgagt ctgatggcac cttatcagat caatcaacag
     3181 gtgattactg ccacgcgtaa ccctgaagtt aaatttatgc actgcctgcc cgccttccat
     3241 aatgagcaca ccacagtagg acgcgagatc gagatggcct acggcctcaa aggactggaa
     3301 gttactgaag aggtttttga atcggcccac tctattgttt tcgatgaagc agaaaaccgg
     3361 atgcacacca ttaaagcggt gatggtagca actctcggcg actaaccctg acgggcatgc
     3421 gtcgacgagt ggcgtatgcc cgtgattatg gagatcacat tatgggtaaa ttcaaatttc
     3481 ccaccgctta taccatcttg ttcgtgctga ttgcgttagt tgcggcaatg acctggattg
     3541 tacctgcggg aaaatatcag atggtgacaa atgccgcact gggaaaggaa gttcctgttg
     3601 caggcactta cgcacctgtt gaggcacaac cacagggtat tacggctgtc ctgttagcgc
     3661 ccatcgacgg cttgtacaac catcaaacct acactgcggg agcgattgat gtcgcgctgt
     3721 ttgtcctgat catcggtgga tttttgggag tagtaaataa aaccggggca atagatgccg
     3781 gtatcgaacg agtgacaact cgccttaatg gtcgggaaga gtggatgatt ccgatcctga
     3841 tggcgctctt cgctgctggc ggtactattt atgggatggc ggaagaatca ctccccttct
     3901 acacgttgct ggtgcccgtc atgatggctg cccgctttga tcctctggtc gctgccgcca
     3961 cagtgttatt gggatcgggc attggcacgc tgggctcaac cattaaccca tttgctaccg
     4021 tcattgctgc caacgccgca gggatcccct ttactcaggg cattttgctg cgtgttattt
     4081 tgctgttagt aggctacgtg atttgtgttt actgggttat gcgttatgcc cgcaaagttc
     4141 gcaacagtcc ggaaagctct atcgtggcgg ataagatggc tgaaaaccag gcgcattttc
     4201 tgggaaatcg ttcagaaacg atgctggaat ttacccccac gcgcaaagcg atcctgatac
     4261 tttttgctgc atcctttgcc tttatgatct acggcgtagc ggtactgggt tggtggatgg
     4321 cggaaatctc tgctgtcttt ctggcagctg cggtcatcgt gggtgtcatt gcccgcatgg
     4381 gcgaagaaac cttcaccagt acctttattg acggcgcgcg ggatctcctt ggtgtggcac
     4441 tgattatcgg catcgcccgc ggtattgtgg tcgtgatgga taacggcatg attacgcaca
     4501 caattctgca cagtgctgag aatctggttt cagggctttc caccactgtt ttcatcaacg
     4561 tgacctattg gctggaagta ttgctctctt ttctggttcc ctcatcttcc ggccttgcag
     4621 tgttaacgat gccaatattg gctccactcg ctgattttgc tcatgtacaa cgcgatctgg
     4681 tggtgaccgc ttatcagtct gcttccgggg tggttaattt gattacgcca acctctgcgg
     4741 tagtgatggg cggtctggcg attgcccgtg taccctgggt ccgttatctg agatgggtag
     4801 caccgctact gctgattctc acgatgttga atatgattgt gctgagtatc gccgcgatga
     4861 tttaacgcga caggccgctt atcaaagcgg ccttcaaatc tggtgtagtg ataaatgaac
     4921 cactctcaca ttataaaatg tgaaaaatat catcaggtaa taataaataa aaggtgcatt
     4981 atgaaggaat acgatgatta ctccgccaaa gaaaagaagc aactggcggt ctgtcagcgc
     5041 ctgattaccg aaaaaagtta tctttcacag gaggaaatcc gccgcgattt gcaaaaccat
     5101 ggctttgaca gtatcagtca gtcaaccgtt tcacgactac tgaaattgct gggagtcata
     5161 aaaattagaa atacaaaagg acaaaaaatt tattcggtga atcctcaatt acttcccaca
     5221 cctgacgcag gtcgatccgt cgctgaaatg gtcctgagtg tcgagcataa tggtgaattt
     5281 atccttatcc ataccgtcgc cggatatggt cgggcagtag cgcgaatcct tgatttccat
     5341 gctttgccag aaattcttgg cgtgattgct ggcagcaata tcgtttgggt cg
//
LOCUS       DADSYM010000203         5378 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2415
            SAMN08039579-rid11723173.denovo.205, whole genome shotgun sequence.
ACCESSION   DADSYM010000203 DADSYM010000000
VERSION     DADSYM010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039579
            Sequence Read Archive: SRR6362533
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5378)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5378)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980162.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 03:39:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,484
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 4,347
            CDSs (with protein)               :: 4,347
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 43 of 85
            Pseudo Genes (incomplete)         :: 37 of 85
            Pseudo Genes (internal stop)      :: 19 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5378
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039579-rid11723173.denovo.205"
                     /strain="LGE2415"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8302 N 95.9628 W"
                     /collection_date="2015-08-24"
     gene            267..1118
                     /locus_tag="J1Y83_004067"
     CDS             267..1118
                     /locus_tag="J1Y83_004067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001195265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HBA7632160.1"
                     /translation="MQKVKLNNGIEMPLLGFGVFQMTDAAECERAVIDAINSGYRLID
                     TAASYQNEIQVGNALKQSGIARNELFVTTKLWLQDTSYEGAKAQFERSLNRLQLDYVD
                     LYLIHQPYGDVHGAWRAMEELQQAGKIRAIGVSNFHPDRLADLIAFNNVVPAVNQVEV
                     NPFNQQLQAVPWMQSRGIQPEAWAPFAEGKNGLFQHPVLTAIGEKYGKSVGQVVLRWI
                     YQRGIVSLAKSVRKERMEENINILDFELSPEDMLQITALDTATSAFFSHRDPAMVEWL
                     TGRKLDV"
     gene            1145..2134
                     /locus_tag="J1Y83_004068"
     CDS             1145..2134
                     /locus_tag="J1Y83_004068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001172283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HBA7632161.1"
                     /translation="MQKRYLGKSGLEVSALGLGCMGLSHGYGPATDTRQAIELIRAAV
                     ERGVTFFDTAEVYGPYLNEEVVGEALKPFRDRVVVATKFGFTFGDDNKQQILNSRPEH
                     IREAVEGSLRRLKTDVIDLLYQHRVDPDVPIEDVAGTVKDLIAEGKVKHFGLSEAGAQ
                     TIRRAHAVQPVTALQSEYSMWWREPEQEILPLLEELGIGFVPFSPLGKGFLTGAIKPG
                     TTFGKDDYRSTVPRFAAQAIEANEKLVTLLGELAAEKGVTSAQIALAWLLAQKPWIVP
                     IPGTTKLHRLEENLAAADIVLSQKDTQQISEALETIKIVGERYSPEHQARVGR"
     gene            complement(2165..3058)
                     /locus_tag="J1Y83_004069"
     CDS             complement(2165..3058)
                     /locus_tag="J1Y83_004069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000910707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBA7632162.1"
                     /translation="MLKENFNELQIFLVVARERSFTKAAGKLGVSQSALSHAMKALEE
                     RLNIRLLTRTTRSVAPTEAGERIIACLEPRIDELEQELESLIQLNGTPSGNIRLSAGE
                     HAARSLIWPKLKPFLREYPEINVELVVDNGFVDIVEGRFDAGIRLGENVDKDMVAVRI
                     GPDMRMAVVGAPAYFAANPAPETPHELQNHRCINMRLPTAGGLYHWEFEREGKPLRVK
                     VDGQLTCSPLPERIDAALSGFGIACVPEDMVQEYIESGKLIQVLQEWCPTFPGYYLYY
                     PSRKQHPPAFALLIDALRYTE"
     gene            complement(3258..4184)
                     /locus_tag="J1Y83_004070"
     CDS             complement(3258..4184)
                     /locus_tag="J1Y83_004070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBA7632163.1"
                     /translation="MMKIEPSILPSLAWFALIVRAGSFSRAASEMGITRAALSQNLKS
                     LEERLNTKLIYRTTRNMSLTEEGQHLYEVLVSALGQIDDALKDVGDTQLEPTGLLRIN
                     SSRVAARMLVEPHIGEFLTRYPKTKIELIMDDGLSNIIADGCDVGIRLEQGLDEHMTA
                     VPVSPLIKLVTVASPDYLKEHGIPETPQELSNHNCLRLRHKSSGALSAWEFSNVVGGN
                     EEFEIEVSGKYISNDDESMIRMALNGTGIIQHLDFAIAEHINAGKLQPILEDWAVSFP
                     GFYIYVSSRVRMPSKVRAFIDFMVEKRVKIES"
     gene            4341..5261
                     /locus_tag="J1Y83_004071"
     CDS             4341..5261
                     /locus_tag="J1Y83_004071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBA7632164.1"
                     /translation="MKTVSIKHTYWDIAADIYFPDDFNSEKKYPAIISAHPIGSCKEQ
                     TSGSVYGAALAKAGFIVIAFDASFQGSSGGEPRYLEDPTMRVKDFIIVVDYLTTLPYV
                     DAGRIGVLGICGGGGYAINAAMTERRIKAIGTVTGANYGRLMREGFTAFNPIGALEAM
                     AQQRTDEANGAKLRVDDLLPSSPEAALEAGLTEIDLFEATEYYRSPRGCAPNGVNRSL
                     FSHQTVAVGWDAFHLAEVLLTQPLMVVVGDRVGAFGAYRDGCEIIGRAASKHKELVVV
                     EGYSHYDLYDKPEPVKQALDKLIPFYKTHL"
BASE COUNT         1501 a         1291 c         1206 g         1380 t
ORIGIN      
        1 gaataataat gacccgtctt taattaactg ctattgggag tacgtcgaag taagtatata
       61 cgccgggata aaccaatata ttttttatgg acattcattt ttattcatcg gactgtaaat
      121 tccggggata tcgaacttgc acgactaatc tcaaccctca aacagattta tgaattttgt
      181 tcataacccc tgacaaatcc tgccgtctaa tcgagctctt ccctttacgt tatgcttctc
      241 atgaacgata acggaaggat aacaccatgc aaaaagtaaa actgaacaac ggtattgaaa
      301 tgcccctgtt gggctttggt gtatttcaga tgaccgatgc cgccgaatgc gaaagagcgg
      361 tcattgacgc tattaacagc ggttatcgac tgattgacac cgccgcctct tatcaaaatg
      421 aaatccaggt cgggaacgca ctgaaacaaa gcggtatcgc ccgtaacgaa ctctttgtca
      481 ccacaaaact ctggttgcag gataccagtt acgaaggggc caaagcccag ttcgaacgct
      541 ctctgaatcg actgcaactt gattacgttg acctgtactt gattcaccag ccatatggcg
      601 atgtccacgg agcctggcgt gccatggaag agctgcaaca ggccggtaaa attcgcgcta
      661 ttggcgtcag taacttccat cctgaccgac tggcagacct tatcgctttt aataacgtcg
      721 ttccggcggt aaaccaggtt gaagttaacc ctttcaatca gcagctacag gccgttccct
      781 ggatgcaaag tcgtggcatt cagccagaag cctgggcacc gtttgcggaa gggaaaaatg
      841 gtctgttcca gcatcctgtt ctcacggcaa ttggcgagaa gtatggcaaa agcgtgggcc
      901 aggtagttct gcgctggatc taccaacgag gcattgtttc actggcaaaa tcagtgcgaa
      961 aagaacgcat ggaagagaac atcaacattc tcgattttga actcagccct gaagatatgc
     1021 tgcaaattac cgccctggat accgcaacca gcgcgttctt ctctcaccgc gatcccgcaa
     1081 tggtggaatg gctgactggc cgcaaacttg atgtttaagc cgcgataaaa gaggtattca
     1141 ttcaatgcaa aaacgttatc tgggtaaatc cggactcgaa gtctccgccc ttgggctcgg
     1201 ttgcatgggc ttaagccacg gctacggccc ggcgaccgat acccgtcagg ctatcgaact
     1261 tattcgtgct gcggttgaac gtggtgtcac cttcttcgat accgccgaag tgtatggtcc
     1321 gtatctgaat gaagaagtgg tcggcgaagc cttaaaaccg tttcgtgacc gcgtggtagt
     1381 tgccactaaa ttcggtttta ccttcggcga tgataataaa cagcagattt taaacagccg
     1441 tccggaacat atccgtgaag ccgttgaagg atcattacgt cgtctgaaaa cagatgttat
     1501 tgacctgctg tatcaacacc gtgttgatcc agatgttcct attgaagatg ttgcaggaac
     1561 ggtgaaagac ctcatcgcag aaggtaaggt caaacatttc ggcctgtctg aagcaggagc
     1621 acaaaccatt cgtcgtgcac atgcggtaca acctgtcacc gccctgcaaa gcgaatattc
     1681 catgtggtgg cgtgagcctg aacaggaaat tctgccgttg cttgaggaac tgggaattgg
     1741 ttttgtcccc ttcagcccac taggtaaagg tttcctgaca ggggcgatta agccgggaac
     1801 cactttcggc aaagatgatt accgcagcac cgtaccgcgt ttcgcagcac aagccattga
     1861 agccaatgaa aaactggtca ccttgttagg tgaactggct gcggagaaag gggtaacgtc
     1921 tgcacaaatc gcactggcat ggctattagc acaaaagccg tggattgttc ccatccctgg
     1981 caccactaaa ctgcaccggc tggaagaaaa cctggccgct gctgacattg ttctttcgca
     2041 gaaagatacg cagcagataa gcgaggcgct ggaaacaatt aaaatcgtcg gtgaacgtta
     2101 ctcgcctgaa catcaggcac gcgtaggccg ctaataccca aacgggtcca ggataaagga
     2161 cccgttattc ggtgtagcga agcgcatcga tcaacaacgc aaaagctggc ggatgctgct
     2221 tgcggctggg gtagtaaaga taatacccag gaaaagttgg acaccactcc tgtaacacct
     2281 gaataagctt gcctgactct atatactcct gaaccatgtc ttcagggaca caagcgatcc
     2341 caaaccctga taacgcggca tcaattcttt ctggcaatgg actacacgtc agttgcccat
     2401 caaccttaac gcgaagcggt ttcccttctc gctcgaactc ccagtggtaa agcccaccgg
     2461 cagttggcaa acgcatgttg atgcagcgat gattttgtag ctcatgaggc gtttcaggcg
     2521 cagggtttgc agcaaaatac gccggagctc ccaccacagc cattcgcata tctggtccaa
     2581 tcctgactgc aaccatatct ttatcaacat tttcacccag tcgtatcccg gcatcaaaac
     2641 ggccttcaac aatatcgaca aaaccgttat ccaccaccag ttcgacattg atttcgggat
     2701 actctctgag aaaaggtttt agcttcggcc agatcagact tcgcgcggca tgttccccgg
     2761 cagataaccg aatattcccg gagggcgtgc cgttcaattg aataagtgat tccagttcct
     2821 gttcaagctc gtcaatacgc ggttcaaggc aggcaatgat tctctccccc gcttctgtgg
     2881 gagccacact gcgggtagtc cgtgtcagga ggcggatatt gagcctttcc tccagagcct
     2941 tcattgcatg gctaagtgca gactgagaga cgccgagttt gcccgctgct ttggtaaaac
     3001 ttcgctctct tgccaccaca agaaagattt gcagttcgtt gaagttttct ttgagcattg
     3061 gcgatttcct tgtagaagca tcctcttcac agacactcat gaaggggggc atagatgcta
     3121 ttattttaac cacattatta gtaacaatga taatctggct gtatcgaaaa taattctaaa
     3181 ctttcaggag atttattatg aataaacata tcctcacgat tgcgccattc ttcttaccga
     3241 tattctgaca cacatcatta actttctatt tttactcttt tctccaccat aaaatcaata
     3301 aaagcacgaa ctttagaggg cattctcacc cgtgatgaaa catatatata aaatccagga
     3361 aatgaaacgg cccaatcctc aagaatcggc tgtaacttgc ccgcattgat atgttcagcg
     3421 attgcaaaat ccagatgttg aatgataccc gttccattca gcgccattcg tatcatgctt
     3481 tcgtcatcat tagaaatata cttacctgac acctctattt cgaactcttc attacccccc
     3541 acaacattcg aaaactccca tgctgagagt gcaccgctac ttttatgccg caaccgaaga
     3601 caattatggt tactcaattc ctgaggagtt tcaggaatac cgtgttcttt taaataatct
     3661 ggcgatgcca cagtaaccag tttaataagc ggagaaacag gaacggcagt catatgctca
     3721 tccagtcctt gctcaagacg tatgcccaca tcacagccgt ccgcaataat gttagataaa
     3781 ccgtcatcca taatgagttc tatctttgtt ttagggtaac gagtaagaaa ctcgccaata
     3841 tgaggctcaa caagcatcct tgctgcaaca cgggaggaat ttattctcaa cagtcctgta
     3901 ggttcaagtt gggtatcacc gacatctttc aacgcatcat caatttgccc caaagccgac
     3961 accagcactt catacagatg ctgtccttcc tcagtaagcg acatattgcg ggtggtacgg
     4021 tatatcagct tggtgttaag acgttcctca agagatttta aattctggga caatgcggcc
     4081 cgcgtaatac ccatttcaga ggctgctctg gaaaaactgc cggcacgaac aatcagcgca
     4141 aaccaggcaa gagaaggcaa aattgaaggc tctattttca tcattgtaaa ccttaactta
     4201 acactgtgat agatattaga ccattgatga taaccaatac aagtcatact attacgccgt
     4261 tcgcgatatg aacacgaatg ccagttgcgg ataatagaaa tgatatttaa atctgtttag
     4321 gcaaatccct ggagcttgaa atgaaaactg tttcgataaa acacacttac tgggatatag
     4381 ctgctgatat ctatttccct gacgatttta atagtgaaaa aaagtatcct gccattatca
     4441 gtgcccatcc aattggaagc tgcaaagaac agacatcggg tagtgtgtat ggtgccgccc
     4501 tggcaaaagc tggctttatc gtcattgctt ttgatgccag ttttcaggga agcagcggtg
     4561 gagaaccgcg ttatctggaa gatccaacaa tgcgagtaaa agacttcatc attgtcgttg
     4621 attaccttac caccttacct tacgttgatg ccgggcgtat tggtgtactg ggtatttgtg
     4681 gtggaggtgg atatgccatt aatgccgcaa tgacagaacg tcggattaaa gccattggta
     4741 ctgtcacggg tgcaaactac gggcgtctta tgcgtgaggg atttacagcg tttaatccta
     4801 tcggagcact cgaagcaatg gcgcaacaaa gaacagacga agctaacggc gcaaaacttc
     4861 gcgtagatga tttgctccct tcttctccgg aagcagctct tgaagcggga ttaacagaaa
     4921 tagacctttt tgaagcaaca gaatattatc gttcgccacg aggctgtgcg cctaatgggg
     4981 taaatcgttc gctattttca catcagactg ttgccgtggg ctgggatgct tttcatcttg
     5041 cagaagtcct gttaactcaa ccactaatgg ttgtagtagg cgatagagtg ggcgcttttg
     5101 gtgcctatcg tgacggctgc gaaattattg gtcgtgcagc ctcaaaacat aaagaactgg
     5161 tcgtggttga aggttattca cattacgacc tgtatgataa gccagaacct gtgaaacagg
     5221 cgctcgataa attaattcct ttttacaaaa cacatttgta ataaccttgc agggagattc
     5281 attatctccc tgacatttat cctttattga ctacacgaca ataataaaat tactcaaaaa
     5341 gaattctatt aaaggaaaaa agcatcaaaa agtagcaa
//
LOCUS       DADSYM010000204         5287 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2415
            SAMN08039579-rid11723173.denovo.206, whole genome shotgun sequence.
ACCESSION   DADSYM010000204 DADSYM010000000
VERSION     DADSYM010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039579
            Sequence Read Archive: SRR6362533
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5287)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5287)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980162.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 03:39:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,484
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 4,347
            CDSs (with protein)               :: 4,347
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 43 of 85
            Pseudo Genes (incomplete)         :: 37 of 85
            Pseudo Genes (internal stop)      :: 19 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5287
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039579-rid11723173.denovo.206"
                     /strain="LGE2415"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8302 N 95.9628 W"
                     /collection_date="2015-08-24"
     gene            117..1475
                     /gene="mltD"
                     /locus_tag="J1Y83_004072"
     CDS             117..1475
                     /gene="mltD"
                     /locus_tag="J1Y83_004072"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase D"
                     /protein_id="HBA7632165.1"
                     /translation="MKAKAILLASVLLVGCQSTGNVQQHAQSLSAAGQGEAAKFTSQA
                     RWMDDGTSIAPDGDLWAFIGDELKMGIPENDRIREQKQKYLRNKSYLHDVTLRAEPYM
                     YWIAGQVKKRNMPMELVLLPIVESAFDPHATSGANAAGIWQIIPSTGRNYGLKQTRNY
                     DARRDVVASTTAALNMMQRLNKMFDGDWLLTVAAYNSGEGRVMKAIKTNKARGKSTDF
                     WSLPLPQETKQYVPKMLALSDILKNSKRYGVRLPTTDESRALARVHLSSPVEMAKVAD
                     MAGISVSKLKTFNAGVKGSTLGASGPQYVMVPKKHADQLRESLASGEIAAVQSTLVAD
                     NTPLNSRVYTVRSGDTLSSIASRLGVSTKDLQQWNKLRGSKLKPGQSLTIGAGSSAQR
                     LANNSDSITYRVRKGDSLSSIAKRHGVNIKDVMRWNSDTANLQPGDKLTLFVKNNNMP
                     DS"
     gene            complement(1524..2294)
                     /locus_tag="J1Y83_004073"
     CDS             complement(1524..2294)
                     /locus_tag="J1Y83_004073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HBA7632166.1"
                     /translation="MTTQSHHDHVEKQFSSQASEYLTSTVHASGRDLQRLAVRLADYP
                     DASVLDMGCGAGHASFVAAQNVSAVVAYDLSAQMLDVVAQAAEARQLKNITTRQGYAE
                     SLPFADNAFDIVISRYSAHHWHDVGAALREVNRILKPGGRLIVMDVMSPGHPVRDIWL
                     QTVEALRDTSHVRNYASGEWLTLISEANLIVDNLITDKLPLEFSSWVARMRTPEALVD
                     AIRIYQQSASTEMKTYFALQNDGSFTSDIIMLEAHKAA"
     gene            complement(2372..3172)
                     /locus_tag="J1Y83_004074"
     CDS             complement(2372..3172)
                     /locus_tag="J1Y83_004074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HBA7632167.1"
                     /translation="MRKNTYAMRYVAGQPAERILPPGSFASIGQALPPGEPLSTEERI
                     RILVWNIYKQQRAEWLSVLKNYGKDAHLVLLQEAQTTPELVQFATANYLAADQVPAFV
                     LPQHPSGVMTLSAAHPVYCCPLREREPILRLAKSALVTVYPLPDTRLLMVVNIHAVNF
                     SLGVDVYSKQLLPIGDQIAHHSGPVIMAGDFNAWSRRRMNALYRFAREMSLRQVRFTD
                     DQRRRAFGRPLDFVFYRGLNVSEASVLVTRASDHNPLLVEFSPGKPDK"
     gene            3413..4327
                     /locus_tag="J1Y83_004075"
     CDS             3413..4327
                     /locus_tag="J1Y83_004075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBA7632168.1"
                     /translation="MKATSEELAIFVSVVESGSFSRAAEQLGQANSAVSRAVKKLEMK
                     LGVSLLNRTTRQLSLTEEGERYFRRVQSILQEMAAAESEIMETRNTPRGLLRIDAATP
                     VVLHFLMPLIKPFRERYPEVTLSLVSSETIINLIERKVDVAIRAGTLTDSSLRARPLF
                     NSYRKIIASPDYISRYGKPETIDDLKQHICLGFTEPASLNTWPIACSDGQLHEVKYGL
                     SSNSGETLKQLCLSGNGIACLSDYMIDKEIARGELVELMADKVLPVEMPFSAVYYSDR
                     AVSTRIRAFIDFLSGHVKTAPGGAVREA"
     gene            complement(4324..5127)
                     /gene="dkgB"
                     /locus_tag="J1Y83_004076"
     CDS             complement(4324..5127)
                     /gene="dkgB"
                     /locus_tag="J1Y83_004076"
                     /EC_number="1.1.1.346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,5-didehydrogluconate reductase DkgB"
                     /protein_id="HBA7632169.1"
                     /translation="MAIPAFGLGTFRLKDDVVISSVKTALELDYRAIDTAQIYDNEAA
                     VGQAIAESGVPRHELYITTKIWIENLSKDKLIPSLKESLQKLRTDYVDLTLIHWPSPS
                     DEVSAEEFMQALLEAKKQGLTREIGISNFTIPLMEKAIAAVGAENIATNQIELSPYLQ
                     NRKVVAWAKQHGIHITSYMTLAYGNALKDKVIARIAAKHNATPAQVILAWAMGEGYSV
                     IPSSTKRENLESNLKAQNLQLDAEDKKAIAALDCNDRLVSPEGLAPEWD"
BASE COUNT         1388 a         1266 c         1318 g         1315 t
ORIGIN      
        1 gaagagcgtt ttgcatggtt aaggtcaaag aaagataggt tctgataaaa ctttcttgtc
       61 atcggctccg ttcgccgtta tgatcggtcg tcttttaagc aactattgac acacacatga
      121 aggcaaaagc gatattactc gcctctgtcc tgctcgtggg ttgccagagt accggcaacg
      181 ttcaacagca cgcacagagc ctttctgcag ctggtcaagg ggaagcagca aagtttacaa
      241 gtcaggcacg atggatggac gatgggacgt ctatcgcgcc agatggtgac ttgtgggctt
      301 tcattggcga cgagctaaag atgggaattc cggaaaacga ccggattcgc gaacagaaac
      361 aaaaatattt acgcaataag agctatctcc acgatgtaac tttacgggca gagccgtata
      421 tgtactggat agccgggcaa gttaaaaaac gtaacatgcc tatggaactg gtactactac
      481 ccatagtgga gagcgctttt gatcctcacg caacgtctgg cgccaatgcc gcgggcatct
      541 ggcagatcat tccgagcacg gggcgcaatt atggtttgaa acagacccgc aattatgacg
      601 cgcgtcgcga tgttgttgct tcaacaactg ccgcgctgaa catgatgcag cgtctgaaca
      661 agatgtttga cggcgactgg cttctgaccg tagcggctta taacagcggc gaaggtcggg
      721 tcatgaaggc aattaaaacg aacaaagcgc gtgggaaatc cacggacttc tggtcgttac
      781 cgttgccgca ggaaacgaag cagtacgtgc ctaaaatgct ggcattgagt gatattctca
      841 aaaacagcaa gcgttatggc gtacgtctgc caacgaccga tgaaagccgt gctctggcac
      901 gtgtgcacct gagcagcccg gttgaaatgg cgaaggttgc agatatggcg gggatttccg
      961 tcagcaagct gaagacattc aacgcaggcg tgaaaggctc cacgctgggc gcaagtggtc
     1021 cgcagtacgt gatggtgcca aagaagcatg cagatcaact gcgtgaatct ctggcttcag
     1081 gcgaaattgc tgctgtacag tcgacgctgg ttgccgacaa tacgccgctt aacagccgtg
     1141 tttacaccgt acgctctggc gacacgcttt caagtatcgc ttcacgtctc ggcgtaagca
     1201 ccaaagattt gcagcagtgg aacaaactgc gcggatctaa gctgaagcca ggccaaagtt
     1261 tgacgattgg tgcaggcagt agcgcacagc gactggcaaa caacagcgat agcattacgt
     1321 atcgtgtgcg caaaggcgat tcgctttcaa gcattgctaa acgccacggc gtgaacatca
     1381 aagatgtaat gcgctggaac agcgacactg caaatctgca accaggcgat aagctgacgt
     1441 tgtttgtgaa aaacaacaac atgccagatt cctgacaaac cagataataa aaaaggcacc
     1501 gattcccccg gtgccttttt tatttatgcc gctttatgtg cttctagcat gatgatatca
     1561 ctggtgaaag agccatcatt ctgcaaggca aaatacgttt tcatctctgt cgatgcgctc
     1621 tgttggtaaa tgcgaatagc gtctactaac gcttctggcg tacgcattct cgctacccat
     1681 gaagaaaatt ccagcggtaa cttatctgta attaaattat caactatcag attggcttca
     1741 ctgattaacg tcaaccactc accgctggcg tagtttcgta cgtgagaggt atcgcgtaat
     1801 gcttctaccg tctgtaacca gatgtcgcgc actggatgac ccggagacat tacgtccatc
     1861 acaatcagcc taccgccagg tttcaatatc ctattcactt ctcgcagtgc tgcaccaaca
     1921 tcatgccagt gatgggcaga ataacggctg ataacaatat caaatgcgtt atcggcaaat
     1981 ggcagacttt cggcataacc ctggcgggtg gtgatatttt tcagttgccg ggcttcggca
     2041 gcttgtgcca cgacatccag catttgggca gataagtcat acgctaccac cgcgctcacg
     2101 ttctgcgcag cgacaaagct ggcatgccct gctccgcagc ccatatcaag tacacttgca
     2161 tcaggataat cagccagacg caccgccagg cgctgtaaat ctcgtccgga tgcatgtacg
     2221 gtactagtca gatattcgct agcttgagag ctaaactgct tttctacgtg gtcatggtgg
     2281 gattgtgttg tcattgcgct gtccttttgt tgtttgagta taaaagggca gcgccaaacg
     2341 ttggtctgcc ctgtggcaga cctgacatat cttatttatc aggcttgccg ggactgaatt
     2401 caacgagtag cggattgtga tcggacgcgc gcgtaaccag tacagaagct tcgctgacgt
     2461 tcagaccacg gtagaaaaca aaatcgagcg ggcgaccaaa cgcccgacgg cgctgatcat
     2521 cggtaaaacg cacctggcgt agcgacattt cccgcgcaaa gcgatataac gcgttcatcc
     2581 ttctacggct ccaggcattg aaatctcccg ccataatgac cgggccactg tggtgagcta
     2641 tctgatcgcc aataggaagt aactgcttac tatagacatc cacgcccaga ctgaagttga
     2701 cggcgtgtat attaaccacc atcaacaggc gggtgtcagg taagggatag accgtcacca
     2761 gtgccgattt cgccagacgc aaaatgggtt ctcgttcacg taacgggcag caatacactg
     2821 gatgtgccgc cgaaagggtc attacgccag aaggatgttg tggcagcacg aaagcgggta
     2881 cctgatcggc ggcaagatag ttagcagtcg caaactgtac taactctggc gttgtctgcg
     2941 cttcctgcaa taacaccaga tgtgcatctt tgccgtagtt ctttaatacc gacaaccatt
     3001 cagcgcgttg ctgtttgtat atgttccaca ccaggatccg aatacgctct tcggtactta
     3061 acggttctcc aggtggtaat gcctggccga tgctcgcaaa agaccccggc ggtaagatcc
     3121 tttccgcagg ttgtccggca acatagcgca tggcataggt gttttttcgc acttttgact
     3181 ttgaaacctc tatcacgtca acccttccgc actcgggagg gttctactgt tatagggatt
     3241 ttagctcact ctttcaacga gcaattgtat attgttatgt aagcaagtgc ttacaccggt
     3301 caggattttg ctcattattg agaacattaa atcggatggc atatctcgca tcctgatggt
     3361 ttaatgacaa ataaattcaa aatatctctt ctattttgca acaggagcaa aaatgaaagc
     3421 cacgtcggaa gaactcgcca tttttgtttc agtcgtcgaa agcggcagct ttagccgggc
     3481 agcggaacaa ttagggcaag caaactcagc ggtaagccgg gcggtgaaaa agctggagat
     3541 gaaacttggc gttagcctgc ttaatcggac cacgcggcaa cttagcctga cggaagaagg
     3601 cgagcgttat tttcgtcgcg tacagtcaat tttgcaggag atggcagcgg cagaatcaga
     3661 aattatggag acgcgtaata caccgcgtgg actgttacgg atcgatgccg caactccagt
     3721 ggtgctgcac tttctgatgc cgttgattaa gccttttcgt gaacgctatc cggaagtcac
     3781 tttatcgcta gtctcctccg aaacgattat taatttgatc gaaagaaaag tggatgtcgc
     3841 gatacgcgcc ggtacgttaa cggattccag cttacgtgcc aggccgctat ttaacagtta
     3901 tcgaaaaatt atcgcctccc ccgattatat ttcccgctac gggaagccag aaacgatcga
     3961 cgatttaaag caacatattt gcctgggatt cactgaaccc gcttccctca atacctggcc
     4021 gatagcctgt agcgatggac aattacatga ggtgaagtac ggtttgtcat ccaatagtgg
     4081 ggaaacactg aaacagcttt gcctgagtgg caacgggatt gcgtgtttgt ccgactacat
     4141 gatcgacaaa gaaatcgctc gcggagaatt ggtggagtta atggcagata aagtgttgcc
     4201 agtagaaatg ccattcagtg cagtctatta cagcgaccgt gcggtaagta cgcgcatccg
     4261 ggcttttatc gatttcctta gcgggcatgt aaaaacagct cccggaggag ctgtcagaga
     4321 ggcttaatcc cattcaggag ccagaccttc cgggctaacc aggcggtcgt tgcaatccag
     4381 tgcggcgatc gcttttttat cttcggcatc aagctgtaaa ttttgtgcct taagattact
     4441 ctccaggttt tcacgtttag tagaagaagg aattactgag taaccttccc ccatagccca
     4501 cgccagaatc acttgagccg gagtcgcatt gtgtttagct gcaatacgag caataacctt
     4561 atctttcagt gcattgccat acgccagagt catataggaa gtaatatgga taccgtgctg
     4621 tttagcccaa gcgaccactt tacggttttg cagataagga gagagttcaa tctggttagt
     4681 agcgatgttt tcagcgccaa cagcagcaat cgctttttcc atcaatggga tcgtgaagtt
     4741 ggaaataccg atctcacgcg tcagcccttg ttttttggct tccagcagcg cctgcataaa
     4801 ctcttcagca gagacttcat cgcttggtga cggccagtgg attagcgtca gatcaacata
     4861 atcggtacgc aatttttgca ggctctcttt cagactcggg atcaatttgt ctttgctgag
     4921 attttcaatc cagattttag tggtgatgta gagttcatga cgtggcacgc cactttctgc
     4981 aatcgcctga cctactgcgg cttcgttatc atagatttgt gcggtatcaa ttgcgcgata
     5041 atcaagttca agcgccgttt tcacagatga aataacaacg tcgtctttca gacggaaagt
     5101 gcctaaacca aatgcaggga tagccataat atgcctcttt taatgctatt ttcgttaact
     5161 gaggtgatta tgggcttcag acagttgtgg aaaaatggcg taaaacgcag aggatttttg
     5221 cgattttggc aataatagaa atacaaacga aaaaaaacct cgagcgttaa ctcgaggctt
     5281 cttaata
//
LOCUS       DADSYM010000205         5253 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2415
            SAMN08039579-rid11723173.denovo.207, whole genome shotgun sequence.
ACCESSION   DADSYM010000205 DADSYM010000000
VERSION     DADSYM010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039579
            Sequence Read Archive: SRR6362533
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5253)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5253)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980162.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 03:39:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,484
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 4,347
            CDSs (with protein)               :: 4,347
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 43 of 85
            Pseudo Genes (incomplete)         :: 37 of 85
            Pseudo Genes (internal stop)      :: 19 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5253
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039579-rid11723173.denovo.207"
                     /strain="LGE2415"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8302 N 95.9628 W"
                     /collection_date="2015-08-24"
     gene            68..1864
                     /locus_tag="J1Y83_004077"
     CDS             68..1864
                     /locus_tag="J1Y83_004077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002954510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aryl-sulfate sulfotransferase"
                     /protein_id="HBA7632170.1"
                     /translation="MFDKYRKTLVAGTVAITLGLSASGVMAAGFKPAPPAGQLGAVIV
                     DPYGNAPLTALVDLDSHVISDVKVTVHGKGEKGVEISYPVGQESLKTYDGVPIFGLYQ
                     KFANKVTVEWKENGKVMKDDYVVHTSAIVNNYMDNRSISDLQQTKVIKVAPGFEDRLY
                     LVNTHTFTAQGSDLHWHGEKDKNAGILDAGPATGALPFDIAPFTFIVDTEGEYRWWLD
                     QDTFYDGRDRDINKRGYLMGIRETPRGTFTAVQGQHWYEFDMMGLVLEDHKLPRGFAD
                     ATHESIETPNGTVLLRVGKSNYRRDDGVHVTTIRDHILEVDKSGRVVDVWDLTKILDP
                     KRDALLGALDAGAVCVNVDLAHAGQQAKLEPDTPFGDALGVGPGRNWAHVNSIAYDAK
                     DDSIILSSRHQGVVKIGRDKQVKWILAPSKGWEKPLASKLLKPVDANGKPITCNENGL
                     CENSDFDFTYTQHTAWISSKGTLTIFDNGDGRHLEQPALPTMKYSRFVEYKIDEKKGT
                     VQQVWEYGKERGYDFYSPITSIIEYQADRNTMFGFGGSIHLFDVGQPTVGKLNEIDYK
                     TKEVKVEIDVLSDKPNQTHYRALLVRPQQMFK"
     gene            1882..2550
                     /locus_tag="J1Y83_004078"
     CDS             1882..2550
                     /locus_tag="J1Y83_004078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbA/DsbL"
                     /protein_id="HBA7632171.1"
                     /translation="MSKLGISSLFKTILLTAALAVSFTASAFTEGTDYMVLERPIPNA
                     DKTLIKVFSYACPFCYKYDKAVTGPVSEKVKDIVAFTPFHLETKGEYGKQASEVFAVL
                     INKDKAAGISLFDANSQFKKAKFAYYAAYHDKKERWSDGKDPAAFIKTGLDAAGMSQA
                     DFEAALKEPAVQETLEKWKASYDVAKIQGVPAYVVNGKYLIYTKSIKSIDAMADLIRE
                     LASK"
     gene            2565..3236
                     /gene="dsbI"
                     /locus_tag="J1Y83_004079"
     CDS             2565..3236
                     /gene="dsbI"
                     /locus_tag="J1Y83_004079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000511507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-disulfide oxidoreductase DsbI"
                     /protein_id="HBA7632172.1"
                     /translation="MGIKGMWKDLRTSPVDTLVRWQEQRLLWLLMAVAMGALIILAHS
                     FFQIYLYMAPCEQCVYIRYAMFVMVIGGLVAAINPKNIILKLIGCVMAFYGSILGLKF
                     SLKLNDIHHAVHNPDPDSLFGVQGCSTDPTFPFNLPLAQWAPNWFKPTGDCGYDAPIV
                     PDGVTLSSTQQWFVEMYQQSEGWYLLPPWHFMNMAQACMLAFGMCLVLLVIMSGAWAL
                     KIIRG"
     gene            complement(3282..4097)
                     /gene="ygiD"
                     /locus_tag="J1Y83_004080"
     CDS             complement(3282..4097)
                     /gene="ygiD"
                     /locus_tag="J1Y83_004080"
                     /EC_number="1.13.11.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311954.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4,5-DOPA dioxygenase extradiol"
                     /protein_id="HBA7632173.1"
                     /translation="MTPLVKDIIMSSPRMPALFLGHGSPMNVLEDNLYTRSWQTLGMT
                     LPRPQAIVVVSAHWFTRGTGVTAMEMPPTIHDFGGFPQALYDTHYPAPGSPALAQHLV
                     ELLAPVPVTLDKEAWGFDHGSWGVLIKMYPDADIPMVQLSIDSSKPAAWHFEMGRKLA
                     ALRDEGIMLVASGNVVHNLRTVKWHGDSSPYPWAASFNEYVKANLTWQGPVEQHPLVN
                     YLDHEGGALSNPTPEHYLPLLYVLGAWDGQEPITIPVDGIEMGSLSMLSVQIG"
     gene            4213..4986
                     /gene="zupT"
                     /locus_tag="J1Y83_004081"
     CDS             4213..4986
                     /gene="zupT"
                     /locus_tag="J1Y83_004081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZupT"
                     /protein_id="HBA7632174.1"
                     /translation="MSVPLILTILAGAATFIGAFLGVLGQKPSNRLLAFSLGFAAGIM
                     LLISLMEMLPAALAAEGMSPVLGYGMFIFGLLGYFGLDRMLPHAHPQDLMQKSVQPLP
                     KSIKRTAILLTLGISLHNFPEGIATFVTASSNLELGFGIALAVALHNIPEGLAVAGPV
                     YAATGSKRTAILWAGISGLAEILGGVLAWLILGSMISPVVMAAIMAAVAGIMVALSVD
                     ELMPLAKEIDPNNNPSYGVLCGMSVMGFSLVLLQTAGIG"
     gene            complement(5044..5214)
                     /locus_tag="J1Y83_004082"
     CDS             complement(5044..5214)
                     /locus_tag="J1Y83_004082"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA7632175.1"
                     /translation="MFIAWYWIVLIALVVVGYFLHLKRYCRAFRQDRDALLEARNKYL
                     NSTREETAEKVE"
BASE COUNT         1351 a         1301 c         1276 g         1325 t
ORIGIN      
        1 ttagcaccct ctcaacagtc gatattcatc attattatat atttgcgtta tataaggaga
       61 tatatccatg tttgataaat atagaaaaac actcgtagcc ggaactgtgg cgataaccct
      121 gggtttgtca gcatcggggg tgatggctgc gggttttaaa ccagcgccgc ctgccgggca
      181 actgggtgcg gtcattgtcg atccctacgg caatgcacca ctgaccgctt tggttgactt
      241 agatagccat gttatttctg acgtcaaagt taccgtccat gggaagggcg aaaaaggcgt
      301 agaaatcagc tatcccgtgg gtcaggaatc actaaaaact tacgatggtg taccgatttt
      361 tggtctttat cagaaatttg ctaacaaagt gaccgttgag tggaaagaaa acggcaaggt
      421 catgaaagat gattatgtgg tgcacacttc ggccatcgtc aataattaca tggataaccg
      481 ctctatctcc gatttacaac agaccaaagt tattaaagtc gcaccgggtt ttgaagatcg
      541 cctctatctg gttaataccc acacctttac cgcccaaggt tccgatctcc actggcatgg
      601 tgagaaagat aaaaatgccg gtatccttga tgcgggtccg gcaactggcg cactcccttt
      661 tgatatcgcg ccattcacct ttatcgtcga taccgaaggt gaataccgct ggtggctgga
      721 tcaagacact ttctacgatg gtcgtgaccg cgatatcaac aagcgtggtt atctgatggg
      781 tatccgcgaa acgccacgcg gcacctttac cgctgtacaa ggtcagcact ggtacgagtt
      841 cgacatgatg ggactggtgc tcgaagatca caaactaccg cgcggatttg ctgacgccac
      901 tcatgagtcc attgagacgc caaatggcac ggtactgttg cgcgtaggta agagtaacta
      961 tcgtcgcgat gacggcgtac acgtcaccac cattcgtgac catatcctcg aagtcgataa
     1021 atctggtcgc gttgtcgatg tatgggatct gaccaagatc ctcgatccga aacgcgatgc
     1081 actgctcggc gcgctggatg caggtgcagt ttgcgttaac gttgaccttg cccatgcagg
     1141 acaacaggca aaactggaac cagatacacc gttcggcgac gcgctgggtg tagggccagg
     1201 ccgcaactgg gcgcacgtga actctatcgc ctatgacgca aaagatgact ctatcatcct
     1261 ctcctctcgc caccagggtg ttgtgaagat tggtcgtgat aagcaagtga aatggatcct
     1321 tgcgccctct aaaggttggg aaaaaccgct ggccagcaag ctgctgaaac cggtggacgc
     1381 taacggtaaa cctattacct gtaacgaaaa cggtctgtgt gaaaactcgg acttcgattt
     1441 tacctacacc cagcataccg cctggatttc cagcaaagga acgctcacca tttttgataa
     1501 tggcgatggt cgtcatctgg aacaacctgc cttaccaacc atgaaatatt cccgctttgt
     1561 ggaatataag attgatgaga agaaaggcac cgttcagcaa gtgtgggaat acggtaaaga
     1621 acgtggctac gatttctata gcccgattac ctccatcatt gaatatcaag ccgaccgtaa
     1681 caccatgttt ggcttcggtg gttctattca tttgttcgat gtcgggcagc caaccgtcgg
     1741 taagttgaac gaaatcgatt acaaaaccaa agaagtgaaa gtggaaatcg acgtgctgtc
     1801 agataaaccc aatcagactc actatcgtgc actgttagtc cgtccacaac agatgttcaa
     1861 ataattaaga agggaataaa aatgtcgaaa ttagggatct catcactatt taaaaccata
     1921 ctgcttaccg cagctttggc tgtttcattt accgcttctg catttaccga aggcacggat
     1981 tacatggttc tggaaagacc tattccgaat gccgacaaaa cgctgattaa ggtatttagc
     2041 tacgcctgcc cgttctgtta caagtacgac aaagcggtta ccggtccggt atcggaaaaa
     2101 gtgaaagata tcgttgcttt cacgccattc catctggaaa caaaaggtga atacggcaaa
     2161 caagcaagcg aagtctttgc tgtcttgatc aataaagata aagcagcagg catttcctta
     2221 tttgatgcta actctcagtt taagaaagcc aagtttgcct actacgcggc ttatcacgac
     2281 aaaaaagaac gctggtctga cggtaaagat cccgctgcct ttatcaaaac tggtctggat
     2341 gccgcaggta tgagtcaggc ggattttgaa gcggcactga aagaaccagc ggttcaagaa
     2401 acactggaaa aatggaaagc ctcttatgat gtcgccaaaa tccagggggt tccagcctat
     2461 gtcgtaaatg gtaagtactt gatctacacc aagagcatca aatccatcga cgcgatggca
     2521 gaccttatcc gtgagttagc aagcaaataa cagggaaaca ggctatgggt attaagggaa
     2581 tgtggaaaga tcttcgcaca tcaccagttg acacactggt cagatggcag gagcaacgct
     2641 tgttgtggtt gttaatggcc gttgcgatgg gcgcgttaat tatcctggcg cactctttct
     2701 tccagatcta tctctacatg gcaccctgtg agcaatgtgt atacattcgc tacgccatgt
     2761 ttgtgatggt tattggagga ttggttgcag cgatcaaccc aaagaacatc atcctaaagc
     2821 taattggctg cgtgatggca ttttacggca gcattttggg actgaagttt tcgctcaaac
     2881 tgaacgatat ccaccatgca gtacataatc cggatcccga ttcactgttt ggcgtacagg
     2941 gttgctctac cgatcccact tttcccttta acctgccgct ggcacagtgg gcaccaaatt
     3001 ggttcaagcc taccggggat tgtggctatg acgcaccaat cgttcctgat ggcgtcacac
     3061 tgagcagcac gcaacagtgg tttgtggaaa tgtatcagca atctgaaggc tggtatctgc
     3121 taccaccatg gcattttatg aatatggctc aggcttgtat gctggctttt ggtatgtgtc
     3181 tggtgctgct ggtcattatg agtggcgcat gggcgcttaa aatcatcaga ggctaactca
     3241 gcaacacccg aagccagtcg ttctggcttc gggttctttt attaacctat ctgcaccgac
     3301 agcatactta aactgcccat ttctatacca tcgaccggaa tggtaattgg ctcctgccca
     3361 tcccacgcac ctaagacata caacaacggc agatagtgct ctggcgttgg gttcgataac
     3421 gcgccacctt catggtcgag ataattcacc agaggatgtt gttccactgg cccttgccac
     3481 gtcagattcg ctttcacata ctcattaaac gacgccgccc acggatacgg tgaactatca
     3541 ccgtgccact tcactgtacg caggttatgc accacgttgc cactggcgac caacattatc
     3601 ccttcatcac gcagcgccgc gagtttgcgc cccatttcga aatgccaggc ggcaggtttg
     3661 ctactgtcga tactcaactg caccatcggg atatcggcgt caggatacat cttaatcagt
     3721 acgccccacg agccgtggtc gaagccccag gcttctttat ccagcgtcac agggaccggc
     3781 gctaacagct caaccagatg ctgtgccagc gcaggtgaac ccggagcagg ataatgcgta
     3841 tcgtacagcg cctgcgggaa gccaccaaag tcatgaatcg tgggcggcat ctccatcgcg
     3901 gtcactcctg ttccacgggt aaaccaatga gccgaaacca ccactattgc ttgcgggcgt
     3961 ggcaatgtca tccccaacgt ctgccagctg cgggtataca aattatcttc cagcacgttc
     4021 atcggactac cgtgacctaa aaacaatgct ggcatacgtg gtgaagacat gatgatatcc
     4081 ttaactaaag gtgtcatttt gatatcctca caatacgctt gttcggcgga gtaagaaccc
     4141 ggataacaat gatgatgatc atcagttatt ttgacgatct gcctgaaggt gaagatttat
     4201 aaggagttgt cgatgtcagt acctctcatt ctgaccatac tggcgggggc agccacgttt
     4261 attggcgcgt ttctcggcgt tctcgggcaa aaaccctcga accgcttact ggcgttttcg
     4321 ctaggatttg cggcggggat catgttgctc atctcattaa tggaaatgct tcctgccgca
     4381 ttagccgccg aaggaatgtc acctgtgttg ggttatggaa tgtttatctt cggtctgctt
     4441 ggctattttg gtctggaccg catgttgcca catgcgcatc cgcaggattt aatgcaaaaa
     4501 tcggtgcagc cgttgccaaa atcgatcaag cgcacagcca ttctgctcac tctcggcatc
     4561 agtctgcata acttcccgga agggattgcc acctttgtca cggcgagcag caacctggag
     4621 ctgggatttg gcatcgccct ggccgtagcg ttgcacaata ttcctgaagg tctggcggtt
     4681 gcaggcccgg tttacgctgc aacaggatcg aaacgtaccg cgattctgtg ggcgggcatt
     4741 tccgggctgg cagaaattct tggtggtgtg ctggcatggt taatcctcgg tagcatgatt
     4801 tctccggtgg tcatggcggc aatcatggcg gcggttgcag gaattatggt ggcgctctcc
     4861 gttgacgaat taatgccgct cgccaaagag atcgacccca ataataaccc cagctatggc
     4921 gtactatgtg gaatgtcagt gatgggattc agtttagtgc tgctacaaac ggcgggaatt
     4981 ggttaaaagc aacatcagaa gttgtccagc gcatggacaa cttctgtcag cttcactaaa
     5041 gtcttattct accttttcgg ctgtctcttc tctcgtactg tttaagtatt tgttccgcgc
     5101 ttcaagcagg gcgtctctgt cctggcgaaa cgcccgacaa tatcgtttca aatgcaggaa
     5161 ataacccacc acaaccagag caatcaatac aatccagtac caggcgataa acatcctttt
     5221 acccttaata tttaacaata ctaaacattt agc
//
LOCUS       DADSYM010000206         4973 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2415
            SAMN08039579-rid11723173.denovo.208, whole genome shotgun sequence.
ACCESSION   DADSYM010000206 DADSYM010000000
VERSION     DADSYM010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039579
            Sequence Read Archive: SRR6362533
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4973)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4973)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980162.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 03:39:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,484
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 4,347
            CDSs (with protein)               :: 4,347
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 43 of 85
            Pseudo Genes (incomplete)         :: 37 of 85
            Pseudo Genes (internal stop)      :: 19 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4973
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039579-rid11723173.denovo.208"
                     /strain="LGE2415"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8302 N 95.9628 W"
                     /collection_date="2015-08-24"
     gene            1..337
                     /locus_tag="J1Y83_004083"
                     /pseudo
     CDS             1..337
                     /locus_tag="J1Y83_004083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000020612.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
     gene            521..1087
                     /gene="slp"
                     /locus_tag="J1Y83_004084"
     CDS             521..1087
                     /gene="slp"
                     /locus_tag="J1Y83_004084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312404.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein Slp"
                     /protein_id="HBA7632176.1"
                     /translation="MNMTKGALILSLSFLLAACSSIPQNIKGNNQPDIQKSFVAVHNQ
                     PGLYVGQQARFGGKVINVINGKTDTLLEIAVLPLDSYAKPDIEANYQGRLLARQSGFL
                     DPVNYRNHFVTILGTIQGEQPGFINKVPYNFLEVNMQGIQVWHLREVVNTTYNLWDYG
                     YGAFWPEPGWGAPYYTNAVSQVTPELVK"
     gene            1240..1770
                     /locus_tag="J1Y83_004085"
     CDS             1240..1770
                     /locus_tag="J1Y83_004085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBA7632177.1"
                     /translation="MFLIITRDTMFFTAMKNILSKGNVVHIQNEEEIDVMLHQNAFVI
                     IDTLMNNVFHSNFLTQIERLKPVHVIIFSPFNIKRCLGKVPVTFVPRTIAIIDFVALI
                     NGSYCSVPEADVSLSRKQHQVLSCIANQMTTEDILEKLKISLKTFYCHKHNIMMILNL
                     KRINELVRHQHINYLV"
     gene            complement(1827..2855)
                     /gene="chuS"
                     /locus_tag="J1Y83_004086"
     CDS             complement(1827..2855)
                     /gene="chuS"
                     /locus_tag="J1Y83_004086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hematinate-forming heme oxygenase ChuS"
                     /protein_id="HBA7632178.1"
                     /translation="MNHYTRWLELKEQNPGKYARDIAGLMNISEAELAFARVTHDAWR
                     MRGDIREILAALESVGETKCICRNEYAVHEQVGAFTNQHLNGHAGLILNPRALDLRLF
                     LNQWASVFHIKENTARGERQSIQFFDHQGDALLKVYATDNTDMAAWSELLARFITDEN
                     TPLELKAVDAPVVQTRADASVVEQEWRAMTDVHQFFTLLKRHNLTRQQAFNLVADDLA
                     CKVSNSALAQILESAQQDGNEIMVFVGNRGCVQIFTGVVEKVVPMKGWLNIFNPTFTL
                     HLLEESIAEAWVTRKPTCDGHVTSLELFAHDGTQIAQLYGQRTEGEQEQAQWRKQIAS
                     LIPEGVTA"
     gene            complement(2904..4886)
                     /locus_tag="J1Y83_004087"
     CDS             complement(2904..4886)
                     /locus_tag="J1Y83_004087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent
                     hemoglobin/transferrin/lactoferrin family receptor"
                     /protein_id="HBA7632179.1"
                     /translation="MSRPQFTSLRLSLLALAVSATLPTFAFATETMTVTATGNARSSF
                     EAPMMVSVIDTSAPENQTATSATDLLRHVPGITLDGTGRTNGQDVNMRGYDHRGVLVL
                     VDGVRQGTDTGHLNGTFLDPALIKRVEIVRGPSALLYGSGALGGVISYDTVDAKDLLQ
                     EGQSSGFRVFGTGGTGDHSLGLGASAFGRTENLDGIVAWSSRDRGDLRQSNGETAPND
                     ESINNMLAKGTWQIDSAQSLSGLVRYYNNDAREPKNPQTVEASDSSNPMVDRSTIQRD
                     AQLSYKLAPQGNDWLNADAKIYWSEVRINAQNTGSSGEYREQITKGARLENRSTLFAD
                     SFASHLLTYGGEYYRQEQHPGGATTGFPQAKIDFSSGWLQDEITLRDLPITLLGGTRY
                     DSYRGSSDGYKDVDADKWSSRAGMTINPTNWLMLFGSYAQAFRAPTMGEMYNDSKHFS
                     IGRFYTNYWVPNPNLRPETNETQEYGFGLRFDDLMLSNDALEFKASYFDTKAKDYIST
                     TVDFAAATTMSYNVPNAKIWGWDVMTKYTTDLFSLDVAYNRTRGKDTDTGEYISSINP
                     DTVTSTLNIPIAHSGFSVGWVGTFADRSTHISSSYSKQPGYGVNDFYVSYQGQQALKG
                     MTTTLVLGNAFDKEYWSPQGIPQDGRNGKIFVSYQW"
BASE COUNT         1370 a         1215 c         1096 g         1292 t
ORIGIN      
        1 tttataactg cacaacaaat accgataatg ttaaaacgcc agacacaccg aagtggcctt
       61 gtgataaccg ggaagaacaa caacccatta attccccatt ctctggagaa ggatacacct
      121 ctgaacaatt tgatttagcg caacaacaac gccaacaaat taatgcgtgc catacgaaca
      181 ccacatatac aaatgcagac tactcaaagt tgaggctcaa cttgtaagtc ttattaataa
      241 cattgaaacg ataagctcga caccacttcc gcaaaagaca caatcaatac ttaatcagat
      301 aaataacatt agacacgaaa agattaaaaa cctctgaata tagattatgg aacaatggat
      361 actcatgccg cgcccaaaaa ttaactgagc tcaactaaac agaagagata taaacatcag
      421 acaggtttac gttactatca ggcatatcgc ctcagaatca gatgaaaact ataaagaaat
      481 atctattatg gttttaatat ttgttgataa ggatagtaac atgaacatga caaaaggtgc
      541 actcatcctc agcctttcat ttttgcttgc cgcatgtagt tcaattccgc aaaatatcaa
      601 aggcaataac caacctgata ttcaaaaaag ttttgttgct gttcataacc agccggggtt
      661 atatgttggt caacaagcgc gctttggtgg gaaggttatc aacgttatca atggcaaaac
      721 ggatacgttg ttagaaatcg ctgtattacc gttggatagc tatgcgaagc ctgatattga
      781 agccaactat cagggccgac tgctcgccag acaaagcggc ttccttgatc cagtgaacta
      841 tcgtaatcac tttgttacca tcctcggtac cattcagggt gaacaacctg gctttatcaa
      901 taaagtcccg tataacttcc tggaagtgaa tatgcagggc atccaggtgt ggcatttgag
      961 agaagtggtt aataccacct ataacttgtg ggattacggc tatggtgcat tctggccgga
     1021 accgggctgg ggggctcctt actacaccaa tgcggtgagt caggtaacac ctgagctggt
     1081 caaataacac caccgaaaga tgcagatgct ttctcagcat ctgcatcatg cattacatca
     1141 aattaataca cagtaagcta actattatta taagccctgt cctgttaatt acctttggca
     1201 aactgattat aaagttaatg tccgtaccag gagtcggtta tgtttcttat aattaccagg
     1261 gatacgatgt ttttcaccgc gatgaaaaac attctgagta aaggtaatgt cgttcatata
     1321 cagaacgaag aagagatcga cgtaatgttg catcagaatg ccttcgtcat tattgataca
     1381 ttaatgaata atgtatttca ttctaatttt ctcactcaaa ttgaacgatt aaaacctgtc
     1441 catgtaatta ttttctcccc ctttaatatt aaacgctgcc tggggaaagt accggtgacc
     1501 tttgttccgc gaactatcgc catcatcgac tttgtcgcac tcatcaatgg cagttactgc
     1561 tctgtgcctg aagcggatgt gtccctctcg cgcaagcagc atcaggttct gagctgtatt
     1621 gcgaatcaaa tgacaacgga agatattctg gagaaactga aaatatcgct aaaaacgttc
     1681 tactgccata aacacaatat catgatgatc ctcaatctta agcggatcaa tgagctggta
     1741 cgccatcagc atattaatta tctggtgtga acgattgaac aatataaaga ggcccagcaa
     1801 cagccagacc tcccgttaat tatacgttat gcagtaacgc cttccggtat caacgaagca
     1861 atttgcttac gccattgcgc ttgctcctgt tcaccttctg tacgttgacc ataaagttgc
     1921 gctatctgcg taccatcatg ggcaaacaat tccaggctgg ttacgtggcc atcgcaggtc
     1981 ggtttacggg taacccaggc ttcagcaatg ctctcttcta atagatgaag ggtaaacgtc
     2041 gggttgaaaa tattcagcca acctttcatt ggcaccactt tttctaccac accggtgaaa
     2101 atctgtacgc agccacggtt gccaacaaac accatgattt cattgccatc ctgctgtgca
     2161 gattcaagaa tttgcgccaa cgcactgttg gatactttgc aggccaaatc gtctgccacc
     2221 agattgaacg cctgttggcg cgtcaggttg tggcgcttga gcaacgtaaa aaactgatga
     2281 acgtcggtca tcgcccgcca ctcttgctcg accacactgg catcggctcg cgtttgaaca
     2341 actggcgcat caacggcttt taactcaagc ggcgtattct catcggtgat aaaccgtgcc
     2401 agaagctcac tccatgccgc catatcggta ttgtcggtgg cataaacttt tagtaatgca
     2461 tcgccctgat gatcaaagaa ctgaatactc tggcgttcgc cacgagccgt gttttctttg
     2521 atgtggaaaa cactggccca ttgattgaga aacagacgta aatccagcgc gcgcggattg
     2581 aggatcaatc cggcatgtcc gttcaaatgc tggtttgtga acgcaccaac ttgctcatgg
     2641 actgcatatt cattacggca aatacatttg gtttcgccaa cactttcgag cgccgccaga
     2701 atttcacgga tatcgccgcg catccgccac gcatcgtgcg tgacgcgtgc aaatgccagt
     2761 tctgcttcgc tgatattcat taaacctgcg atgtcacgcg cgtactttcc gggattttgt
     2821 tcttttaact caagccagcg tgtgtagtgg ttcattgtct ctcttccttc cagataaatg
     2881 ccccgaaata tcggggcaga tgattaccat tgataactca cgaaaatttt tccgttacga
     2941 ccatcctgtg ggatgccttg cggcgaccag tactctttat cgaaggcgtt gcccaatacc
     3001 agagtagtgg tcatgccttt gagcgcctgc tgcccttgat aactgacgta gaaatcattc
     3061 acaccatagc caggttgttt gctgtagctg ctgctgatat gtgttgagcg atcggcaaac
     3121 gtaccgaccc aaccaacaga gaagccactg tgagcgatcg gaatattcag ggtactggta
     3181 acggtatccg ggttaatgct ggagatatat tccccggtat cggtgtcttt gccgcgggta
     3241 cggttatagg ccacatcaag gctaaacaga tcagtggtat atttcgtcat cacatcccag
     3301 ccccagattt tggcgttcgg gacgttatac gacatagttg tcgccgccgc gaaatcgacg
     3361 gtcgtggaga tataatcttt cgctttggta tcaaagtagc tggctttaaa ttccagagca
     3421 tcattggaca acatcaggtc atcaaaacgc agcccaaaac cgtactcctg agtttcgtta
     3481 gtttccggac gtaagttcgg gtttggcacc caatagttgg tatagaagcg accaatcgag
     3541 aagtgtttag aatcgttata catttcgccc atcgtcgggg cgcggaatgc ctgagcatat
     3601 gagccaaata acatcagcca gttggtcggg ttgatagtca tccccgcacg agatgaccat
     3661 ttgtcggcat caacatcttt gtagccgtcg ctgctaccgc gataactgtc atagcgggtt
     3721 ccgccaagca gggtaatcgg cagatcgcgt aaggtgatct catcttgtag ccagccagag
     3781 ctaaaatcga tttttgcttg cgggaagccc gtcgtcgcgc cacccggatg ttgttcctga
     3841 cgataatact caccgccata tgtcagtaag tgagaagcga aactgtcggc aaatagagtg
     3901 gaacggttct ccagccttgc tccttttgtt atctgttcac gatactcgcc tgaactcccc
     3961 gtgttttgcg cattaatacg gacttccgac cagtaaattt ttgcatctgc atttaaccag
     4021 tcgttaccct gcggggcgag tttataagaa agctgcgcat cgcgttgaat tgttgaacga
     4081 tcgaccatcg ggttgctgct atcagaagct tcaacggtct gcggattttt tggttcacgc
     4141 gcgtcgttgt tgtagtaacg cactaaaccg ctcagagact gggctgaatc aatttgccag
     4201 gtccctttcg ccagcatgtt attaatggac tcgtcattcg gcgcggtttc accattgctc
     4261 tggcgtaaat caccccgatc gcgactggac caggccacaa taccatccag attttcagtt
     4321 cgcccaaacg cgctcgcgcc taatcccagg ctatggtccc ccgtgccgcc agtaccaaag
     4381 acacgaaaac cactgctttg tccttcctgc aataaatctt ttgcatcgac cgtatcgtag
     4441 gagatcactc cacccagcgc gccactgcca tacagtaatg ctgaaggtcc gcgaacaatc
     4501 tcaacacgct tgatcagcgc cggatcgaga aaagtgccat tcaggtgtcc ggtatccgtt
     4561 ccctggcgaa caccatcgac aagaaccagc acgccgcgat gatcatagcc acgcatattt
     4621 acatcctgac cgttggttcg tccggtacca tcaagagtaa ttccaggaac atgacgcagc
     4681 aaatcagtgg ctgaagtagc agtttgattt tcaggagcgg aagtgtcgat aacgctgacc
     4741 atcataggcg cttcgaagga actacgtgca ttccccgttg ccgtaacggt catggtttca
     4801 gtagcaaaag caaacgttgg caaggtggca gaaacagcca aagccaacaa actcaaacgc
     4861 aacgaggtaa attgcggacg tgacatagcg attctccatg aggaaaataa aaaatgctgg
     4921 ctgcctgggt taataccggg gtggctggcg aaaaataaat ttatttacaa ttc
//
LOCUS       DADSYM010000207         4941 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2415
            SAMN08039579-rid11723173.denovo.209, whole genome shotgun sequence.
ACCESSION   DADSYM010000207 DADSYM010000000
VERSION     DADSYM010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039579
            Sequence Read Archive: SRR6362533
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4941)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4941)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980162.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 03:39:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,484
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 4,347
            CDSs (with protein)               :: 4,347
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 43 of 85
            Pseudo Genes (incomplete)         :: 37 of 85
            Pseudo Genes (internal stop)      :: 19 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4941
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039579-rid11723173.denovo.209"
                     /strain="LGE2415"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8302 N 95.9628 W"
                     /collection_date="2015-08-24"
     gene            complement(68..1120)
                     /gene="aroG"
                     /locus_tag="J1Y83_004088"
     CDS             complement(68..1120)
                     /gene="aroG"
                     /locus_tag="J1Y83_004088"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001109196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroG"
                     /protein_id="HBA7632180.1"
                     /translation="MNYQNDDLRIKEIKELLPPVALLEKFPATENAANTVAHARKAIH
                     KILKGNDDRLLVVIGPCSIHDPVAAKEYAARLLTLREELQDELEIVMRVYFEKPRTTV
                     GWKGLINDPHMDNSFQINDGLRIARKLLLDINDSGLPAAGEFLDMITPQYLADLMSWG
                     AIGARTTESQVHRELASGLSCPVGFKNGTDGTIKVAIDAINAAGAPHCFLSVTKWGHS
                     AIVNTSGNGDCHIILRGGKEPNYSAKHVAEVKEGLNKAGLPAQVMIDFSHANSSKQFK
                     KQMDVCTDVCQQIAGGEKAIIGVMVESHLVEGNQSLESGEPLAYGKSITDACIGWDDT
                     DALLRQLANAVKARRG"
     gene            1436..1816
                     /locus_tag="J1Y83_004089"
     CDS             1436..1816
                     /locus_tag="J1Y83_004089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA7632181.1"
                     /translation="MKMTKLATLFLTATLSLASGAALAADSGAQSNNGQANAAADAGQ
                     VAPDARENVAPNNVDNNGVNTGSGSTMLHPDGSSMNNDGMTKDEEHKNTMCKDGRCPD
                     INKKVQTGDGINNDVDTKTDGTTQ"
     gene            1930..2871
                     /gene="zitB"
                     /locus_tag="J1Y83_004090"
     CDS             1930..2871
                     /gene="zitB"
                     /locus_tag="J1Y83_004090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family zinc transporter ZitB"
                     /protein_id="HBA7632182.1"
                     /translation="MAHSHSHTSSHLPEDNNARRLLYAFGVTAGFMLVEVIGGFLSGS
                     LALLADAGHMLTDTAALLFALLAVQFSRRPPTIRHTFGWLRLTTLAAFVNAIALVVIT
                     ILIVWEAIERFRTPRPVEGGMMMAIAVAGLLANILSFWLLHHGSEEKNLNVRAAALHV
                     LGDLLGSVGAIIAALIIIWTGWTPADPILSILVSLLVLRSAWRLLKDSVNELLEGAPV
                     SLDIAELKRRMCREIPEVRNVHHVHVWMVGEKPVMTLHVQVIPPHDHDALLDQIQHYL
                     MDHYQIEHATIQMEYQPCHGPDCHLNEGVSGHSHHHH"
     gene            complement(2868..3587)
                     /locus_tag="J1Y83_004091"
     CDS             complement(2868..3587)
                     /locus_tag="J1Y83_004091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide riboside transporter PnuC"
                     /protein_id="HBA7632183.1"
                     /translation="MDFFSVQNILVHIPIGAGGYDLSWIEAVGTIAGLLCIGLASLEK
                     ISNYFFGLINVTLFGIIFFQIQLYASLLLQVFFFAANIYGWYAWSRQTSQNEAELKIR
                     WLPLPKALSWLAVCVVSIGLMTVFINPVFAFLTRVAVMIMQALGLQVAMPELQPDAFP
                     FWDSCMMVLSIVAMILMTRKYVENWLLWVIINVISVVIFALQGVYAMSLEYIILTFIA
                     LNGSRMWINSARERGSRALSH"
     gene            complement(3625..4668)
                     /gene="nadA"
                     /locus_tag="J1Y83_004092"
     CDS             complement(3625..4668)
                     /gene="nadA"
                     /locus_tag="J1Y83_004092"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HBA7632184.1"
                     /translation="MSVMFDPDTAIYPFPPKPTPLSIDEKAYYREKIKRLLKERNAVM
                     VAHYYTDPEIQQLAEETGGCISDSLEMARFGAKHPASTLLVAGVRFMGETAKILSPEK
                     TILMPTLQAECSLDLGCPVEEFNAFCDAHPDRTVVVYANTSAAVKARADWVVTSSIAV
                     ELIDHLDSLGEKIIWAPDKHLGRYVQKQTGADILCWQGACIVHDEFKTQALTRLQEEY
                     PDAAILVHPESPQAIVEMADAVGSTSQLIAAAKTLPHQRLIVATDRGIFYKMQQAVPD
                     KELLEAPTAGEGATCRSCAHCPWMAMNGLQAIAEALELEGSNHEVYVDERLRERALVP
                     LNRMLDFAATLRG"
BASE COUNT         1246 a         1287 c         1160 g         1248 t
ORIGIN      
        1 gcgttttatc aggcctccgg tgattaatcg gatacgccac tctgacggcg catccgacaa
       61 ttaaacttta cccgcgacgc gcttttactg cattcgccag ttgacgtaac agagcatcgg
      121 tatcatccca gccaatgcag gcatcggtga tgctcttgcc ataggccagc ggttccccac
      181 tctcgaggct ctgattgcct tccaccagat ggctttccac catcacgcca ataatggcct
      241 tttcgccacc ggcaatctgc tggcaaacgt cagtacaaac atccatctgc tttttgaatt
      301 gttttgacga gttagcatgg ctgaaatcga tcatcacctg tgctggcagg cctgctttgt
      361 tcagcccttc ttttacttca gcaacgtgct tcgcgctgta gttaggctct ttaccgccgc
      421 gcagaatgat atggcaatcg ccgttaccgc tggtattcac aatcgccgaa tgcccccatt
      481 tcgttacgga caggaagcag tgcggcgcac cggcggcatt aatggcatcg atagccactt
      541 taatcgtacc gtcggtgcca tttttgaaac ctaccggaca agaaagacca gacgccagtt
      601 cgcggtgcac ctgcgattcg gtggtacgtg caccaattgc gccccagctc atcaggtcag
      661 cgagatattg tggggtgatc atatccagga attcacccgc cgctggcaga ccgctgtcgt
      721 taatatcgag cagcaatttg cgggcaatac gcagaccgtc gttgatctgg aagctgttat
      781 ccatatgcgg atcgttaatc agccctttcc agcccaccgt agtacgcggc ttttcaaaat
      841 agacgcgcat cacgatttcc agctcatctt gcagctcttc acgcagcgtc agcaagcgag
      901 cggcatactc tttagccgcg acaggatcat gaattgagca tgggccaatc accaccaaca
      961 ggcgatcatc attacctttc aggattttat ggatcgcttt tcgggcatgg gcgaccgtat
     1021 tcgcggcatt ttcagtagca gggaattttt ccagcaatgc gacaggagga agtaactctt
     1081 tgatttcttt gatgcgtaaa tcgtcgttct gataattcat gtctgttcca gtgttgccat
     1141 acttatctta gtgaatgcaa tacttccaat ctatatcttc cgtcagaatg tgtaaacggg
     1201 gttttacact atgaacggat taatcctgga attagcaaga aaaacgccag attgtcgcga
     1261 aaaacgagat ctctcctaca atttctaact gtaactcctt tgtttgttaa ttatttcaag
     1321 attctctgct gggtatcttt gcctggctga aatcttaaac cattgcctta tattcacctg
     1381 caaatgcact gttggaagag gttatccgac ataacgacca taacaggagc atcctatgaa
     1441 aatgacaaaa ctggccacac tatttttgac tgccactcta agccttgcca gcggtgccgc
     1501 actggctgcc gatagcggag cgcaaagcaa taacggccag gcaaacgccg cagctgatgc
     1561 gggccaggta gcccctgatg cccgtgaaaa tgtcgcgcca aataacgtcg acaataacgg
     1621 ggtaaatacc ggttctggca gcacaatgct gcatccggat ggttcttcaa tgaacaatga
     1681 cggcatgacc aaagatgaag agcacaagaa caccatgtgc aaagatggtc gctgcccgga
     1741 tatcaataaa aaagtccaaa ccggtgatgg catcaacaat gatgtcgata ccaaaaccga
     1801 cggtaccacg cagtaaccct gcgaaatagc caataatggg agagcggagt ctctcccttt
     1861 taatttttcc cttctaaaag cagtatgatg tcataaagtt aagtgagaat aattaaggga
     1921 tgactcgtca tggcgcactc acactcacac acttcctcac acctgccaga agataataat
     1981 gctcgtcgct tgttgtatgc tttcggcgtg actgccgggt ttatgctcgt agaagtcatt
     2041 ggcggttttc tttccggttc tctggcattg ctggccgatg cgggtcatat gttgaccgat
     2101 actgccgccc tgcttttcgc cctactcgcc gtgcaatttt cacgtcgacc tcccaccatt
     2161 cgccacactt tcggttggct aagattgacc acgctcgcag catttgtgaa tgctatcgcc
     2221 ctggtggtga ttaccatttt gattgtctgg gaggcgatag aacgcttccg cacaccgcgt
     2281 ccggtcgagg gcggcatgat gatggcaatt gccgtggctg ggctgttggc aaatatactt
     2341 tctttctggt tacttcatca cggtagcgaa gagaaaaacc tcaacgtgcg agcagcggca
     2401 ctgcatgtgc tcggggacct gctgggttcg gttggggcta ttattgccgc gttgattatt
     2461 atctggaccg gctggacacc tgctgacccc attctctcaa tactggtgtc gcttctggtt
     2521 ctgcgtagcg cgtggcgact gttgaaagat agcgtgaatg aattacttga aggtgcaccg
     2581 gtatcgctgg atatcgccga actgaagcgt cgtatgtgcc gggaaatccc ggaagtccgc
     2641 aatgtgcacc atgtacatgt atggatggta ggcgagaagc cggtgatgac gctgcatgtg
     2701 caggtgatcc cgccacacga tcacgatgcc ttgttagatc agatccaaca ctacctgatg
     2761 gatcactatc agattgagca cgccactatt cagatggaat atcaaccttg tcatgggcca
     2821 gactgccacc tgaatgaagg ggtgtctggc cattcacatc atcaccatta atgggacagc
     2881 gcgcgtgaac ctctttcgcg tgcgctgttg atccacatcc ggctgccgtt gagggcaata
     2941 aaggtcagga tgatgtactc cagagacatg gcgtaaacgc cctgaagtgc aaaaataacg
     3001 acgctaatca cgttaataat cacccacaac agccagtttt ccacatactt acgcgtcatc
     3061 agaatcattg ccacgataga taacaccatc atgcatgaat cccagaacgg gaaagcgtcc
     3121 ggctgtagtt caggcatcgc aacctgtaat cctaatgctt gcatgatcat gactgccacg
     3181 cgggtcaaaa atgcaaacac cggattgata aataccgtca tcagaccaat cgaaacaacg
     3241 caaaccgcca accagcttag cgccttcggc aatggcaacc agcgaatttt caactccgcc
     3301 tcgttctgac tggtttgtcg cgaccacgca taccagccgt aaatattcgc ggcaaagaaa
     3361 aacacctgta atagcaggct ggcatacagc tgaatctgaa agaaaataat gccaaacaag
     3421 gtgacgttga tcaggccaaa gaagtagtta ctgatcttct ccagactggc aaggccaata
     3481 cacagcaacc cggcgatcgt gcctaccgct tcgatccatg agagatcata accgcctgcc
     3541 cctattggta tatgtaccag gatattctgc acactaaaaa aatccatcgt tcccccaaag
     3601 cgtaatgtga cgccttatta tcaattatcc acgtagtgta gccgcaaaat ccagcatacg
     3661 attgagcggc accagcgccc tctctcgcag cctttcatca acataaacct cgtgattgct
     3721 tccttccagt tctaatgcct ctgcgatggc ctgaaggcca ttcatggcca tccacgggca
     3781 atgcgcgcag ctgcgacagg ttgcgccctc acccgcggtt ggtgcttcca gtaactcttt
     3841 atctggcacc gcctgctgca ttttgtagaa aatgccccga tcggttgcca cgataagccg
     3901 ctgatgtggc aatgttttcg cagcagcgat cagttgactg gtggaaccga ccgcatccgc
     3961 catctctaca atagcttgcg gtgattctgg atgcaccagt atggcagcat ccgggtattc
     4021 ttcttgcaag cgggttaacg cttgagtctt aaattcatca tgcacaatgc aggcaccctg
     4081 ccagcataaa atgtctgcgc ccgtctgttt ttgcacgtaa cgccccagat gtttgtcagg
     4141 tgcccagatg attttttcac ccaaactatc cagatgatca ataagttcga cggcaatgct
     4201 tgaagttacc acccaatctg cgcgcgcttt taccgcagca gaagtgttgg cgtagacgac
     4261 gacagtacga tcgggatggg catcgcaaaa tgcgttaaat tcttcaacag ggcagccgag
     4321 atccagtgaa cattcagcct gaagtgtagg catcagaatt gttttttccg gactgagaat
     4381 tttggcggtt tctcccataa atctcacccc agcgactaac aacgtagaag cgggatgctt
     4441 tgcaccgaag cgcgccattt ccagagaatc agaaatacag ccaccggttt cttctgccag
     4501 ttgttgaatt tcgggatcgg tatagtagtg ggcaaccatc accgcattac gttcttttag
     4561 tagacgtttt atcttctcgc ggtaatacgc tttttcatca atgcttaacg gcgtcggctt
     4621 cggggggaaa ggataaatcg ccgtgtctgg atcaaacatc acgctcatct taccatctcg
     4681 ttttacaggc ttaacgttaa aaccgactta agcgtgatat tcgctattat cggcgtaatt
     4741 tgttttacat gctaaacaag atagtcgaat ggtggagaaa agtcactcga attacgtatg
     4801 agatgccgaa tgccacactt tgcgtcttat tcctccgacg tagagttgtt tacgcagcaa
     4861 atgcggagta tgtcatcagg gtcaagaatt gaaattgaga gatgcctgaa tagcaaaaaa
     4921 gcgcctttag ggcgcttttt t
//
LOCUS       DADSYM010000208         4912 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2415
            SAMN08039579-rid11723173.denovo.210, whole genome shotgun sequence.
ACCESSION   DADSYM010000208 DADSYM010000000
VERSION     DADSYM010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039579
            Sequence Read Archive: SRR6362533
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4912)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4912)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980162.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 03:39:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,484
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 4,347
            CDSs (with protein)               :: 4,347
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 43 of 85
            Pseudo Genes (incomplete)         :: 37 of 85
            Pseudo Genes (internal stop)      :: 19 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4912
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039579-rid11723173.denovo.210"
                     /strain="LGE2415"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8302 N 95.9628 W"
                     /collection_date="2015-08-24"
     gene            127..1461
                     /locus_tag="J1Y83_004093"
     CDS             127..1461
                     /locus_tag="J1Y83_004093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBA7632185.1"
                     /translation="MMLDTVDEKKKGVHTRYLILLIIFIVTAVNYADRATLSIAGTEV
                     AKELQLSAVSMGYIFSAFGWAYLLMQIPGGWLLDKFGSKKVYTYSLFFWSLFTFLQGF
                     VDMFPLAWAGISMFFMRFMLGFSEAPSFPANARIVAAWFPTKERGTASAIFNSAQYFS
                     LALFSPLLGWLTFAWGWEHVFTVMGVIGFVLTALWIKLIHNPTDHPRMSAEELKFISE
                     NGAVVDMDHKKPGSAAASGPKLHYIKQLLSNRMMLGVFFGQYFINTITWFFLTWFPIY
                     LVQEKGMSILKVGLVASIPALCGFAGGVLGGVFSDYLIKRGLSLTLARKLPIVLGMLL
                     ASTIILCNYTNNTTLVVMLMALAFFGKGFGALGWPVISDTAPKEIVGLCGGVFNVFGN
                     VASIVTPLVIGYLVSELHSFNAALVFVGCSALMAMVCYLFVVGDIKRMELQK"
     gene            1477..2247
                     /gene="garL"
                     /locus_tag="J1Y83_004094"
     CDS             1477..2247
                     /gene="garL"
                     /locus_tag="J1Y83_004094"
                     /EC_number="4.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxyglucarate aldolase"
                     /protein_id="HBA7632186.1"
                     /translation="MNNDVFPNKFKAALAAKQVQIGCWSALSNPISTEVLGLAGFDWL
                     VLDGEHAPNDISTFIPQLMALKGSASAPVVRVPTNEPVIIKRLLDIGFYNFLIPFVET
                     KEEAEQAVASTRYPPEGIRGVSVSHRANMFGTVADYFAQSNKNITILVQIESQQGVDN
                     VDAIAATEGVDGIFVGPSDLAAALGHLGNASHPDVQKAIQHIFNRASAHGKPSGILAP
                     VEADARRYLEWGATFVAVGSDLGVFRSATQKLADTFKK"
     gene            2277..3167
                     /gene="garR"
                     /locus_tag="J1Y83_004095"
     CDS             2277..3167
                     /gene="garR"
                     /locus_tag="J1Y83_004095"
                     /EC_number="1.1.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-3-oxopropionate reductase"
                     /protein_id="HBA7632187.1"
                     /translation="MTMKVGFIGLGIMGKPMSKNLLKAGYSLVVADRNPEAIADVIAA
                     GAETAATAKAIAEQCDVIITMLPNSPHVKEVALGENGIIEGAKPGTVLIDMSSIAPLA
                     SREISEALKAKGIDMLDAPVSGGEPKAIDGTLSVMVGGDKTIFDKYYDLMKAMAGSVV
                     HTGEIGAGNVTKLANQVIVALNIAAMSEALTLATKAGVNPDLVYQAIRGGLAGSTVLD
                     AKAPMVMDRNFKPGFRIDLHIKDLANALDTSHGVGAQLPLTAAVMEMMQALRADGLGT
                     ADHSALACYYEKLAKVEVTR"
     gene            3264..4409
                     /gene="garK"
                     /locus_tag="J1Y83_004096"
     CDS             3264..4409
                     /gene="garK"
                     /locus_tag="J1Y83_004096"
                     /EC_number="2.7.1.165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417593.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate 2-kinase"
                     /protein_id="HBA7632188.1"
                     /translation="MKIVIAPDSYKESLSASEVAQAIEKGFREIFPDAQYVSVPVADG
                     GEGTVEAMIAATQGAERHAWVTGPLGEKVNASWGISGDGKTAFIEMAAASGLELVPAE
                     KRDPLVTTSRGTGELILQALESGATNIIIGIGGSATNDGGAGMVQALGAKLCDANGNE
                     IGFGGGSLNTLNDIDISGLDPRLKDCVIRVACDVTNPLVGDSGASRIFGPQKGASEAM
                     IVELDNNLSHYADVIKKALHVDVKDVPGAGAAGGMGAALMAFLGAELKSGIEIVTTAL
                     NLEEHIHDCTLVITGEGRIDSQSIHGKVPIGVANVAKKYHKPVIGIAGSLTNDVGVVH
                     QHGIDAVFSVLTSIGTLDEAFRGAYDNICRASRNIAATLAIGMRNAG"
     gene            4442..4818
                     /gene="rnpB"
                     /locus_tag="J1Y83_004097"
     ncRNA           4442..4818
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="J1Y83_004097"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
BASE COUNT         1144 a         1177 c         1408 g         1183 t
ORIGIN      
        1 tgattattat tctcgcgctg cttatactaa ttcccgagtt cattgtcgta acggcataaa
       61 tgaaactcat aaataacacc ttccccataa taataaaaat cagcataagt acccgaggta
      121 aataaaatga tgctggacac cgttgacgaa aaaaagaaag gcgtgcatac ccgctattta
      181 atattactga ttatttttat tgttaccgcc gttaactacg ccgatcgtgc aacgctgtct
      241 attgctggta ccgaagtggc aaaagagttg cagttaagtg cggtttcgat gggttacatc
      301 ttctctgctt ttggctgggc ctacttgctg atgcaaattc ccggcggctg gctgcttgat
      361 aagtttggct cgaagaaagt ttacacttac agcctctttt tctggtcgct gttcaccttc
      421 ctgcaaggct ttgtcgatat gtttccgctg gcctgggctg ggatctccat gttctttatg
      481 cgatttatgc tggggttctc ggaagcgcca tcattcccgg cgaacgcccg tattgtcgcc
      541 gcctggttcc cgacgaaaga acgtggtact gcctccgcca tctttaactc ggcgcaatat
      601 ttctcgttgg cgctcttttc gccgctgctt ggctggctga ctttcgcctg gggctgggag
      661 catgtcttta ccgttatggg ggtgattggt tttgtgttga cggcgctgtg gatcaagttg
      721 attcataacc cgacagatca cccacgtatg tctgcagaag agctgaagtt tatctcggaa
      781 aatggcgcgg tggtcgatat ggaccacaaa aagccgggta gtgcggcagc aagcggaccc
      841 aaactgcatt acatcaagca attgctctct aaccgcatga tgctgggcgt atttttcgga
      901 caatatttta tcaacaccat cacctggttc ttcctcacct ggttcccaat ttatctggtg
      961 caggaaaaag gcatgtcgat tctgaaagtg ggtctggtcg cctcgattcc tgcactgtgt
     1021 ggctttgcgg gcggcgtgct gggaggtgtc ttctcggatt atctgatcaa acgcggtttg
     1081 tccctgaccc tggcacgtaa gctaccaatt gtgctgggaa tgttgctggc ttccaccatc
     1141 atcttatgta actacaccaa caacaccacg ctggtggtca tgctgatggc gctggctttc
     1201 tttggcaaag gatttggtgc gctgggctgg ccagtgattt ctgacaccgc gccgaaagag
     1261 attgttggcc tctgcggcgg cgtctttaac gtctttggca atgttgcctc cattgtcact
     1321 ccactggtga ttggctacct ggtaagtgaa ctgcactcct tcaatgcagc actggttttt
     1381 gtgggatgtt cagcgctgat ggcgatggtc tgctacctct tcgtagtcgg cgacattaaa
     1441 cgtatggaat tgcagaaata agcaaaggta taagcgatga ataacgatgt tttcccgaat
     1501 aaattcaaag ccgcactggc tgcgaaacag gtacaaattg gttgctggtc agctctctct
     1561 aacccgatta gcactgaagt tcttggtttg gctgggtttg actggctggt gctggatggt
     1621 gaacatgcgc caaacgatat ctccacgttt attccgcagt taatggccct gaaaggcagc
     1681 gccagcgcgc cagtagtgcg agtgccgacc aacgagccgg taattattaa gcgtcttctg
     1741 gatatcggtt tctataactt cctgattcct tttgtagaaa caaaagagga agcagagcag
     1801 gcggtggcat caactcgtta cccaccggaa ggcattcgcg gcgtctccgt ttctcaccgc
     1861 gccaatatgt ttggcaccgt ggcggattat ttcgctcagt cgaacaagaa catcactatt
     1921 ctggtccaga tagaaagtca gcagggcgta gataacgtcg atgctattgc cgctaccgaa
     1981 ggtgtagacg gcatcttcgt cggccccagc gatctggctg cggcattagg ccatctcggc
     2041 aatgcttcgc acccggatgt acaaaaagca attcagcata tttttaaccg tgccagcgcg
     2101 cacggcaaac ccagcggcat cctcgcacca gtcgaggctg atgcgcgtcg ttatctggaa
     2161 tggggcgcga cgtttgtggc cgtcggcagc gatctcggcg tcttccgctc tgccactcag
     2221 aaactggctg atacctttaa aaaataacca ccaccgcaat aagagagatg attgatatga
     2281 ctatgaaagt tggttttatt ggcctgggga ttatgggtaa accaatgagt aaaaaccttc
     2341 tgaaagcagg ttactcgctg gtggttgctg accgtaaccc agaagctatc gctgacgtga
     2401 ttgctgccgg tgcagaaaca gccgctacgg ctaaagcgat cgctgaacag tgcgacgtca
     2461 tcatcaccat gctgccaaac tcccctcatg tgaaagaggt ggcgctgggt gagaatggca
     2521 ttattgaagg cgcgaagcca ggtacggtat tgatcgatat gagttctatc gcaccgctgg
     2581 caagccgtga aatcagcgaa gcgctgaaag cgaaaggcat tgatatgctg gacgccccgg
     2641 tgagcggcgg tgaaccgaaa gccatcgacg gtacgctgtc agtgatggtg ggcggcgaca
     2701 agactatttt cgacaaatac tatgatttga tgaaagcgat ggcgggttcc gtggtgcata
     2761 ccggggaaat cggtgcaggt aacgtcacca aactggcaaa tcaggtcatt gtggcgctga
     2821 atattgctgc gatgtcagaa gcgttaacgc tggcaaccaa agcgggcgtt aacccggatc
     2881 tggtgtatca ggcaattcgc ggtgggctgg cgggcagtac cgtgctggat gccaaagcgc
     2941 cgatggtgat ggaccgcaac ttcaaaccgg gcttccgtat tgatctgcat attaaggatc
     3001 tggcgaatgc gctggatact tctcacggcg tcggcgcgca actgccgctc acagctgcgg
     3061 ttatggagat gatgcaggca ctgcgtgcag atggtttagg aacggcggat catagcgccc
     3121 tggcgtgcta ctacgaaaaa ctggcgaaag tcgaagttac tcgctaatga cgtcgtgccc
     3181 ggatggcgta ttgcaatccg ggcctggaat ccaggccgaa taagagaaac gccctccggc
     3241 gtcatgtggt aacaggcata ggtatgaaaa tcgtaatcgc cccagactct tataaagaaa
     3301 gtttatctgc cagcgaggtt gcgcaggcga tagaaaaagg atttcgggaa atttttcctg
     3361 atgcacagta tgtttctgtt ccggttgccg acggtggtga aggaacggtg gaagcgatga
     3421 ttgcagccac ccagggggct gaacgtcacg cctgggttac agggccgctg ggcgagaaag
     3481 tgaatgccag ttgggggatc tccggcgatg gcaaaaccgc gtttatcgaa atggcggcgg
     3541 ccagtgggct ggaactggta cctgcggaaa aacgcgatcc actcgtgacc acttcacgcg
     3601 gtactggcga gttaatcctg caggcgctgg agagcggtgc gacaaacatt attatcggca
     3661 ttggcggcag cgccaccaac gacggcggcg caggcatggt acaggcgctg ggggcgaaat
     3721 tatgcgatgc caacggcaat gaaattggtt ttggcggcgg tagtcttaat actctgaatg
     3781 atattgatat ttccggtctc gatccgcgct taaaagattg tgtcatccgc gtcgcttgtg
     3841 atgtcaccaa cccgttagtc ggcgacagtg gtgcttcgcg catctttggc ccacaaaagg
     3901 gagccagtga ggcgatgatt gtcgagctgg acaataacct ctctcactat gccgatgtca
     3961 ttaaaaaagc gctgcatgtt gatgtgaaag atgtccccgg cgcaggagct gcgggcggta
     4021 tgggcgcggc gctgatggcg tttcttggtg cagaactgaa aagtggtatt gaaatcgtca
     4081 ctacggcgct gaatctggag gaacatattc acgattgtac gctggtgatc actggtgaag
     4141 ggcgtattga tagccagagt attcacggca aggtgccgat tggtgtcgca aacgtggcga
     4201 agaagtacca taaaccggtg attggcattg cgggtagcct gaccaatgat gttggcgttg
     4261 tacatcagca tggcattgat gcggtcttca gcgtactgac cagcattggt acgttagacg
     4321 aagcattccg cggagcttat gacaatatct gccgtgcttc acgtaatatc gccgcgacac
     4381 tggcgattgg aatgcgcaac gcggggtgac aagggcgcgc aaaccctcta tactgcgcgc
     4441 cgaagctgac cagacagtcg ccgcttcgtc gtcgtcctct tcgggggaga cgggcggagg
     4501 ggaggaaagt ccgggctcca tagggcaggg tgccaggtaa cgcctggggg ggaaacccac
     4561 gaccagtgca acagagagca aaccgccgat ggcccgcgta agcgggatca ggtaagggtg
     4621 aaagggtgcg gtaagagcgc accgcgcggc tggtaacagt ccgtggcacg gtaaactcca
     4681 cccggagcaa ggccaaatag gggttcataa ggtacggccc gtactgaacc cgggtaggct
     4741 gcttgagcca gtgagcgatt gctggcctag atgaatgact gtccacgaca gaacccggct
     4801 tatcggtcag tttcacctga tttacgtaaa aacccgcttc ggcgggtttt tgcttttggt
     4861 gcccagggta agatgaatat ctacggtatt aataacagac tttgtaagaa gt
//
LOCUS       DADSYM010000209         4849 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2415
            SAMN08039579-rid11723173.denovo.211, whole genome shotgun sequence.
ACCESSION   DADSYM010000209 DADSYM010000000
VERSION     DADSYM010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039579
            Sequence Read Archive: SRR6362533
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4849)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4849)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980162.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 03:39:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,484
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 4,347
            CDSs (with protein)               :: 4,347
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 43 of 85
            Pseudo Genes (incomplete)         :: 37 of 85
            Pseudo Genes (internal stop)      :: 19 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4849
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039579-rid11723173.denovo.211"
                     /strain="LGE2415"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8302 N 95.9628 W"
                     /collection_date="2015-08-24"
     gene            12..620
                     /locus_tag="J1Y83_004098"
     CDS             12..620
                     /locus_tag="J1Y83_004098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001416650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HBA7632189.1"
                     /translation="MCLGIGYAVIRRTIFIVYPPILSNGMFNFIVMQTLFYLPFFILG
                     ALAFIFPHLKALFTTPSRGCTLAAALAFVAYLLNQRYGSGDAWMYETESVITMVLGLW
                     MVNVVFSFGHRLLNFQSARVTYFVNASLFIYLVHHPLTLFFGAYITPHITSNWLGFLC
                     GLIFVVGIAIILYEIHLRIPLLKFLFSGKPVVKRENDKAPAR"
     gene            complement(628..2109)
                     /locus_tag="J1Y83_004099"
     CDS             complement(628..2109)
                     /locus_tag="J1Y83_004099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D family protein"
                     /protein_id="HBA7632190.1"
                     /translation="MMKKTPTSTKDSLPNKEMNDLPRLASAVLPLCSQHPGQCGLFPL
                     EKSLDAFAARYRLAEMAEHTLDVQYYIWQDDMSGRLLFSALLAAAKRGVRVRLLLDDN
                     NTPGLDNILRLLDSHPRIEVRLFNPFSFRLLRPLGYITDFSRLNRRMHNKSFTVDGVV
                     TLVGGRNIGDAYFGAGEEPLFSDLDVMAIGPVVEDVADDFARYWYCKSVSPLQQVLDV
                     PEGEMADRIELPASWHDDAMTHRYLRKMESSPFIHHLVDGTLPLIWAKTRLLSDDPAK
                     GEGKAKRHSLLPQRLFDIMGSPSERIDIISSYFVPTRAGVAQLLRMVRKGVKIAILTN
                     SLAANDVAVVHAGYARWRKKLLRYGVELYELKPTREQSSTLHDRGITGNSGASLHAKT
                     FSIDGKTVFIGSFNFDPRSTLLNTEMGFVIESETLAQLIDKRFIQSQYDAAWQLRLDR
                     WGRINWVDRHAKKEIILKKEPATSFWKRVMVKLASILPVEWLL"
     gene            complement(2051..2584)
                     /gene="ymdB"
                     /locus_tag="J1Y83_004100"
     CDS             complement(2051..2584)
                     /gene="ymdB"
                     /locus_tag="J1Y83_004100"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyl-ADP-ribose deacetylase"
                     /protein_id="HBA7632191.1"
                     /translation="MKTRIHVVQGDITKLAVDVIVNAANPSLMGGGGVDGAIHRAAGP
                     ALLDACLKVRQQQGDCPTGHAVITLAGDLPAKAVVHTVGPVWRGGEQNEDQLLQDAYL
                     NSLRLVAANSYTSVAFPAISTGVYGYPRAAAAEIAVKTVSEFITRHALPEQVYFVCYD
                     EENAHLYERLLTQQGDE"
     gene            complement(2679..2990)
                     /locus_tag="J1Y83_004101"
     CDS             complement(2679..2990)
                     /locus_tag="J1Y83_004101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HBA7632192.1"
                     /translation="MFRPFLNSLMLGSLFFPFIAIAGSSAQGGVIHFYGQIVEPACDV
                     STQSSPVEMNCPQNGSVPRKTYSSKALMSGNVKNAQIASVKVQYLDKQKKLAVMNIEY
                     N"
     gene            complement(3111..3443)
                     /gene="csgC"
                     /locus_tag="J1Y83_004102"
     CDS             complement(3111..3443)
                     /gene="csgC"
                     /locus_tag="J1Y83_004102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli assembly protein CsgC"
                     /protein_id="HBA7632193.1"
                     /translation="MNALLLLAALSSQITFNTTQQGDMYTIIPEVTLTQSCLCRVQIL
                     SLREGSSGQSQTKQEKTLSLPANQLIALTKLSLNISPDDRVKIVVTVSDGQSLHLSQQ
                     WPPSSEKS"
     gene            complement(3502..3960)
                     /gene="csgA"
                     /locus_tag="J1Y83_004103"
     CDS             complement(3502..3960)
                     /gene="csgA"
                     /locus_tag="J1Y83_004103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curlin major subunit CsgA"
                     /protein_id="HBA7632194.1"
                     /translation="MKLLKVAAIAAIVFSGSALAGVVPQYGGGGGNHGGGGNNSGPNS
                     ELNIYQYGGGNSALALQADARNSDLTITQHGGGNGADVGQGSDDSSIDLTQRGFGNSA
                     TLDQWNGKDSTMTVKQFGGGNGAAVDQTASNSSVNVTQVGFGNNATAHQY"
     gene            complement(4001..4456)
                     /gene="csgB"
                     /locus_tag="J1Y83_004104"
     CDS             complement(4001..4456)
                     /gene="csgB"
                     /locus_tag="J1Y83_004104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curlin minor subunit CsgB"
                     /protein_id="HBA7632195.1"
                     /translation="MKNKLLFMMLTILGAPGIAAAAGYDLANSEYNFAVNELSKSSFN
                     QAAIIGQAGTNNSAQLRQGGSKLLAVVAQEGSSNRAKIDQTGDYNLAYIDQAGSANDA
                     SISQGAYGNTAMIIQKGSGNKANITQYGTQKTAIVVQRQSQMAIRVTQR"
BASE COUNT         1237 a         1198 c         1055 g         1359 t
ORIGIN      
        1 cggtgatttt tttgtgcctc ggcatcggtt atgcggtaat aagaagaacg atttttattg
       61 tgtatccacc cattctgagt aacggcatgt tcaattttat tgtcatgcaa acgttatttt
      121 atttgccgtt ctttatcctc ggcgcactgg ctttcatttt ccctcatctt aaagccttgt
      181 ttaccacgcc gtctcgtggc tgtacccttg cagcagcatt ggcgtttgtc gcttatttac
      241 tcaaccagcg ctatggcagt ggcgatgcct ggatgtatga aaccgagtcg gtgatcacta
      301 tggtcctcgg tctgtggatg gtgaatgtgg tcttctcctt tggccaccgt ttgcttaact
      361 tccagtcagc gcgggtgact tattttgtta acgcatcgct gtttatctat ctggttcacc
      421 acccgttaac gctgtttttc ggcgcataca ttacaccaca catcacctcc aactggcttg
      481 gttttctctg tggcctgata ttcgtagtag ggattgcgat aattctgtat gaaattcatt
      541 tacgcatccc attattgaag tttttgttct ctggtaaacc ggttgttaag cgtgagaacg
      601 ataaagcacc agcccgttaa gtcgcgttta caataaccat tccacgggca atatcgacgc
      661 cagtttgacc ataacccgct tccagaaact ggtggcgggt tcttttttga gaataatctc
      721 tttctttgca tgacgatcaa cccagttgat ccgtccccac ctgtccagac ggagctgcca
      781 ggccgcatca tactggctct gaataaagcg tttatcaatt aactgtgcca gcgtttcgct
      841 ctctatcacg aagcccattt cagtattgag caacgttgaa cgcggatcga aattgaaaga
      901 gccgataaac accgttttac catcgatact aaaggtttta gcatgcaggc tggcccctga
      961 attaccggtt atgccgcgat cgtgtaacgt actactttgt tcacgcgtcg gcttgagctc
     1021 atataattcc accccatagc ggagcaattt tttgcgccag cgcgcgtatc ctgcatggac
     1081 gacagcaaca tcgttagcgg caagagaatt ggttaggatc gcaatcttta ccccttttct
     1141 caccatccgt aagagttgcg ccacacctgc gcgtgtcggt acaaaatagg aagagataat
     1201 atcaatgcgt tcactgggtg agcccatgat atcgaacagg cgctgcggta acagtgaatg
     1261 ccgttttgcc ttgccctccc ctttcgccgg atcatcactt aataaacgtg ttttcgccca
     1321 gataagcggc aacgttccat caaccagatg atgtataaac ggactggatt ccattttgcg
     1381 taaataacga tgcgtcatgg catcgtcatg ccaggaggcg ggtaactcga tgcgatctgc
     1441 catttcacct tccgggacat ccagcacctg ctgtaagggt gaaaccgatt tgcaatacca
     1501 atagcgggcg aaatcatcgg caacgtcctc taccacgggt cctattgcca tgacatctaa
     1561 atccgaaaaa agtggctctt cccctgcacc aaaataggca tcaccaatat ttcgtcctcc
     1621 caccaacgtc accacgccat cgacagtgaa acttttattg tgcatacggc gattgagacg
     1681 ggaaaagtcg gtgatataac caagcggacg tagcaagcga aacgagaaag gattaaaaag
     1741 tcggacttca atgcgtggat ggctgtcaag caagcgtaaa atgttatcaa gtccgggcgt
     1801 attgttgtcg tccagcaaca aacgtacgcg aacgccacgc tttgctgcgg ctaacagggc
     1861 ggaaaacagt aaccgacccg acatatcgtc ctgccagatg taatactgaa catccagcgt
     1921 atgctctgcc atttcagcca gacgataccg ggcggcaaac gcatccagac ttttctccag
     1981 agggaaaagt ccacactgac cgggatgttg cgaacacagt ggcagcaccg cgctcgccag
     2041 ccgaggcaaa tcattcatct ccttgttggg taaggagtct ttcgtagagg tgggcgtttt
     2101 cttcatcata acagacaaag tatacctgtt cgggtaaagc gtgacgggta ataaattctg
     2161 aaacggtttt tactgcgatt tcagccgctg ccgcacgagg ataaccataa accccagtac
     2221 tgattgcagg aaaagccact gacgtatagc tgtttgccgc caccagtcgt aggctattga
     2281 gataggcatc ctgcaaaagc tggtcttcgt tttgttcacc accacgccag actggcccga
     2341 cggtgtgcac tacggcttta gcgggaagat cgcctgcaag cgtaataacg gcatgccccg
     2401 tagggcaatc gccctgctgc tgcctgactt ttaaacaggc atccagcagg gccggacccg
     2461 ctgcgcgatg aatggcccca tcgacgccgc cgcctcccat taatgacgga ttagccgcat
     2521 tcacaatcac atcaacggcc agtttagtaa tatcaccctg cacaacatga atacgcgttt
     2581 tcataacgcc tccttacacc ttcttgtttc ctaagtgtac agcaggcttt ttggttaaat
     2641 cgcatttttc tgctcagcaa cgtgagttgt tcagaaactt agttatattc gatgttcatt
     2701 acagccagct ttttctgttt atcaagatac tgcactttta ctgaggctat ttgcgcgttt
     2761 ttgacattgc cgctcattaa cgctttgctg gaataggttt tacgcggaac agaaccattc
     2821 tgtgggcagt tcatttctac gggtgatgac tgggtgctga cgtcacatgc cggttccaca
     2881 atttggccat aaaaatggat cacgccccct tgcgcgctgc ttcctgcaat ggcaataaaa
     2941 ggaaaaaaca aactgccgag cataagagag tttaaaaaag gtctgaacat ttattctatt
     3001 tcctcaatga ttaatcatcc ttgagggttg tgttatccat actttcaggt tggctgattc
     3061 gcagcagacc attctctcca ggttcatctt atgctcgata tttcaacaaa ttaagacttt
     3121 tctgaagagg gtggccattg ttgtgataaa tgaagtgact gtccatcaga aacagtaaca
     3181 actattttca cccgatcgtc cggggaaata tttaaactca acttcgtcaa agcaatgagt
     3241 tgattagcag gcaatgagag ggtcttttct tgcttcgtct gactttgccc tgaactgcct
     3301 tcgcgcaggg ataatatttg tactctgcac agacaagatt gagtaagagt gacttcagga
     3361 ataatggtgt acatatcccc ttgctgggtc gtattaaagg ttatctgact ggaaagtgcc
     3421 gcaaggagta ataacgcatt catattcttc tcccgaaaaa aaacagggct tgcgccctgt
     3481 ttctttcata ctgatgatgt attagtactg atgagcggtc gcgttgttac caaagccaac
     3541 ctgagtgacg ttaacgctgg agttagatgc agtctggtca acagcagcac cgttaccgcc
     3601 accgaactgt ttaacagtca tagtagaatc tttaccattc cactgatcaa gagtagcgct
     3661 gttgccgaaa ccacgctgag tcagatcgat tgagctgtca tcagaacctt ggccaacatc
     3721 tgcgccgtta ccgccgccgt gctgggtaat ggtcagatca gagttacggg cgtcagcttg
     3781 cagagcaaga gcagagttac cgccaccgta ctggtaaata ttcagctctg aattcgggcc
     3841 gctgttatta ccgccaccac cgtggttgcc accgccgccg ccgtactgag gaacaacacc
     3901 tgccagagcg ctaccagaga atacgattgc tgcaattgct gctactttta aaagtttcat
     3961 gtaaaacccc catcggattg atttaaaagt cgaatggaaa ttaacgttgt gtcacgcgaa
     4021 tagccatttg cgactgtctc tgcactacaa ttgccgtttt ttgagtacca tactgtgtaa
     4081 tatttgcttt attaccagaa cctttctgga taatcatcgc agtattacca taagcacctt
     4141 gcgaaatact ggcatcgttg gcactgcccg cctgatcaat atatgcaagg ttataatctc
     4201 ctgtctgatc aatctttgcc cggttgctac taccttcttg cgcaacaacc gccaaaagtt
     4261 ttgagcctcc ctgccgtaac tgagcactat tattagtccc agcttgacca attatggctg
     4321 cctgattaaa tgaagactta ctcaattcat ttaccgcaaa gttatattct gaattagcta
     4381 aatcataacc tgctgcggct gcaatcccag gcgcacccag tattgttaac atcataaata
     4441 acaatttgtt tttcatgttg tcaccctgga cctggtcgta catttaagaa attaaatcat
     4501 ttcaacttgg ttgttaacgc aacctgtatt ttgttaacgc tgcgttacga tggaaagtat
     4561 gtctgcggaa atatttttaa taactcaccc gcgcgttgta tttttctttt tttgttcata
     4621 ccaaagtatt aaaacccacg taaatacgct gttatctacg caaaaaatat tttgttttct
     4681 tttaaatctc cgttttccgc tatcaaaaat caccagacag tcattcttct tgcccgtcgc
     4741 tgattgctgc gatatgtctt gcgcacaagc cgtgacaaac ctcgtctttt tgcagtaaaa
     4801 aattgtccac ggtggtatgg agaaaaaaca agaacgtttt tcatgacga
//
LOCUS       DADSYM010000210         4722 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2415
            SAMN08039579-rid11723173.denovo.212, whole genome shotgun sequence.
ACCESSION   DADSYM010000210 DADSYM010000000
VERSION     DADSYM010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039579
            Sequence Read Archive: SRR6362533
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4722)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4722)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980162.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 03:39:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,484
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 4,347
            CDSs (with protein)               :: 4,347
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 43 of 85
            Pseudo Genes (incomplete)         :: 37 of 85
            Pseudo Genes (internal stop)      :: 19 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4722
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039579-rid11723173.denovo.212"
                     /strain="LGE2415"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8302 N 95.9628 W"
                     /collection_date="2015-08-24"
     gene            130..1464
                     /locus_tag="J1Y83_004105"
     CDS             130..1464
                     /locus_tag="J1Y83_004105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HBA7632196.1"
                     /translation="MNNDNTDYVSNESGTLSRLFKLPQHGTTVRTELIAGMTTFLTMV
                     YIVFVNPQILGAAQMDPKVVFVTTCLIAGIGSIAMGIFANLPVALAPAMGLNAFFAFV
                     VVGAMGISWQTGMGAIFWGAIGLFLLTLFRIRYWMISNIPLSLRIGITSGIGLFIALM
                     GLKNTGVIVANKDTLVMIGDLSSHGVLLGILGFFIITVLSSRHFHAAVLVSIVVTSCC
                     GLFFGDVHFSGVYSIPPDISGVIGEVDLSGALSLELAGIIFSFMLINLFDSSGTLIGV
                     TDKAGLIDSNGKFPNMNKALYVDSVSSVAGAFIGTSSVTAYIESTSGVAVGGRTGLTA
                     VVVGVMFLLVMFFSPLVAMVPPYATAGALIFVGVLMTSSLARVNWDDFTESVPAFITT
                     VMMPFTFSITEGIALGFMSYCIMKVCTGRWRDLNLCVVVVATLFALKIILVD"
     gene            1517..1969
                     /locus_tag="J1Y83_004106"
     CDS             1517..1969
                     /locus_tag="J1Y83_004106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1198 domain-containing protein"
                     /protein_id="HBA7632197.1"
                     /translation="MIWIMLATLAVVFVVGFRVLTSGARKAIRRLSDRLNIDVVPVES
                     MVDQMGKSAGDEFLRYLHRPDESHLQNAAQVLLIWQIVIVDGSEQNMLQWHRILQKAR
                     LAAPITDAQVRLALGFLRETEPEMQDINAFQMRYNAFFQPAEGVHWLH"
     gene            complement(2085..2408)
                     /locus_tag="J1Y83_004107"
     CDS             complement(2085..2408)
                     /locus_tag="J1Y83_004107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator"
                     /protein_id="HBA7632198.1"
                     /translation="MTAKTKFKSPAFEAIHSAAAGLSSVDAISAETMRTFDKACLTSV
                     QDLQPVEIKALREKLKVSQPVFAHYLNTSVSTVQKWESGAKRPNGISLKLLSIVQKHG
                     LEVLL"
     gene            complement(2392..2745)
                     /locus_tag="J1Y83_004108"
     CDS             complement(2392..2745)
                     /locus_tag="J1Y83_004108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HBA7632199.1"
                     /translation="MFKTKWFAREARSHAITDEELCRAILETEQGKADVLGGGVFKKR
                     LHKNRERAIILAKGGSNWFYTFLYAKQDMSNINSQELAGFRELAKHYAFLTKAQLTAM
                     INTKELTEICYDCKN"
     gene            2979..4169
                     /gene="nepI"
                     /locus_tag="J1Y83_004109"
     CDS             2979..4169
                     /gene="nepI"
                     /locus_tag="J1Y83_004109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine ribonucleoside efflux pump NepI"
                     /protein_id="HBA7632200.1"
                     /translation="MSEYIEENRGADAITRPNWSAVFSVAFCVACLIIVEFLPVSLLT
                     PMAQDLGISEGVAGQSVTVTAFVAMFASLFITQTIQATDRRYVVILFAVLLTLSCLLV
                     SFANSFSLLLIGRACLGLALGGFWAMSASLTMRLVPPRTVPKALSVIFGAVSIALVIA
                     APLGSFLGELIGWRNVFNAAAAMGVLCIFWIIKSLPSLPGEPSHQKQNTFRLLQRPGV
                     MAGMIAIFMSFAGQFAFFTYIRPVYMNLAGFGVDGLTLVLLSFGIASFVGTSLSSFIL
                     KRSVKLALAGAPFVLALSALVLTLWGSDKIVATGVAIIWGLTFALIPVGWSTWITRSL
                     ADQAEKAGSIQVAVIQLANTCGAAIGGYALDNIGLTSPLMLSGTLMLLTALLVTAKVK
                     MKKS"
     gene            complement(4210..4503)
                     /locus_tag="J1Y83_004110"
     CDS             complement(4210..4503)
                     /locus_tag="J1Y83_004110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA7632201.1"
                     /translation="MKPTMLLMITVFLIFPAISQAESPFSSLQSAKEKTTVLQDLRKI
                     CTPQASLSDEAWEKLMLSDENNKQHIREAIVAMERNNQSNYWEALGKVECPDM"
BASE COUNT         1016 a         1062 c         1188 g         1456 t
ORIGIN      
        1 aagaaacgtc gcgagattat taaagaatat ccattaatgt gcaattgaaa tgtgataatt
       61 atcactgaac cgttgttgtc atttgtttta caaaagcaag ggattaaatc tcctccaatg
      121 gacaaaaaaa tgaataatga caataccgat tacgtgagta atgaatcagg gacgctttcg
      181 cgattattta aactacctca gcatgggacc accgtccgca cagaattgat tgcggggatg
      241 accacttttt taaccatggt gtacatcgtt tttgtgaacc cgcaaatcct cggcgcggca
      301 caaatggacc cgaaagtggt gtttgttacc acctgtttga ttgccggtat cggcagtatt
      361 gcgatgggga tatttgctaa cttacccgtg gcgctggctc cggcaatggg gctgaacgcc
      421 ttctttgcgt tcgtggtcgt gggggcgatg ggtatctcct ggcagaccgg gatgggcgcg
      481 atattctggg gcgcaattgg gctatttttg ctcacgctgt ttcgtatccg ttactggatg
      541 atctccaaca ttccattaag tttacgtatt ggcatcacca gcggaatcgg attatttatt
      601 gccttaatgg gattaaaaaa cactggcgtt attgtcgcca ataaagacac cctggtgatg
      661 attggcgatt taagttctca cggcgtgttg ttaggtattt tagggttttt tattataact
      721 gtgttgtcat cacgtcattt tcatgccgcg gtgcttgttt ctattgtggt gacgtcttgc
      781 tgtggattat ttttcggtga tgttcatttt agcggtgtct attccattcc gcctgatatt
      841 agcggcgtca ttggtgaagt ggatttgagc ggtgcgttat cacttgaact cgccggtatc
      901 attttctcct ttatgctgat caacttattt gattcatcag ggacattaat tggtgtgact
      961 gataaagctg gcttaataga tagtaacggt aaattcccca atatgaataa ggcgctgtat
     1021 gttgatagcg tcagttcggt ggcgggtgcg tttatcggta cctcgtctgt taccgcctat
     1081 attgaaagta cttcaggtgt ggctgtcggt ggtcgcacgg ggctgacggc tgtcgtagtc
     1141 ggcgttatgt tcctgttagt tatgttcttc tcaccgctgg tggcgatggt tcctccttac
     1201 gcaaccgccg gagcgttaat ctttgttggc gtgctgatga cttcaagcct ggcgcgcgtt
     1261 aactgggatg attttaccga atcggtgcct gcgtttatta ccacggtgat gatgcccttt
     1321 actttctcga tcaccgaagg gattgcacta ggctttatgt cgtactgcat catgaaagtg
     1381 tgcaccgggc gctggcgcga tctgaacctg tgtgtggtgg tggtcgcaac tctgtttgca
     1441 ctgaagatta ttctggtgga ttagtaactg gcgagggttt ccagatatca tgagttctga
     1501 ttacgcagga gaactcatga tctggataat gctcgccacg ctggcggtag tgtttgtggt
     1561 tggttttcgg gtgctgacat ccggggccag aaaagcgatt cgccgtctca gcgatcggct
     1621 gaacatcgat gtcgtacccg tggagtcgat ggtcgatcaa atgggaaagt cagccggtga
     1681 cgaattttta cgttatttgc atcgcccgga tgagtcgcac ctgcaaaacg ccgcgcaggt
     1741 gttgctcatc tggcaaattg tcattgtcga tggtagcgaa cagaacatgc tgcaatggca
     1801 tcggatttta caaaaagccc gccttgccgc gccgattact gatgctcagg tcaggctggc
     1861 gctgggtttt ctgcgcgaaa ccgaacctga aatgcaggat atcaatgctt ttcagatgcg
     1921 ctataacgcg ttctttcagc ctgccgaggg cgttcactgg ctgcattaaa accgtttcgt
     1981 cttatcagcc tacagaccgc gcctgacctg gtgggtggat aaggcgtgta cgtcgcatcc
     2041 ggtatcggaa ttgacagtag gtgaacgcat tatctacgtt ctatttacag cagcacctcc
     2101 agcccgtgtt tttgcacaat gctgagcagt tttaatgaaa tgccattggg gcgtttagcg
     2161 ccactttccc atttttgtac agttgaaacg ctggtattga gatagtgagc gaagacgggc
     2221 tgactcactt ttaatttttc ccgcagtgct tttatctcaa caggttgtaa gtcttgcacg
     2281 ctggtcaggc aggctttgtc gaaagtacgc atcgtttcgg cagaaatagc atcgacgctg
     2341 gacaatccag ccgctgcgct gtgaatagct tcaaatgcag gacttttaaa tttagttttt
     2401 gcagtcatag cagatctctg tcagttcttt agtgttaatc attgctgtca gttgggcttt
     2461 cgtcaggaag gcataatgct ttgcaagttc acgaaaccca gccagttcct gactattaat
     2521 gttcgacata tcctgttttg catataaaaa cgtgtaaaac caattgcttc cccctttcgc
     2581 cagaataatt gcacgctcac gatttttgtg aaggcgcttt ttaaaaacgc caccaccaag
     2641 tacatcagct ttaccttgtt ccgtttctaa aatggcccgg cacaactctt catctgttat
     2701 tgcatgagac ctggcttccc ttgcaaacca tttggtttta aacacgcgca tatcgttgag
     2761 atcctgttat taaagtatat caccaagtgc aatactttat cccttattcc caacctaatc
     2821 gcgaaaatgc atatcacctt gccctatgat ttttatttct tcgagcagcc tcgaactttt
     2881 cccacaacac cgtaaaagtt gcaaaaacgt taaatacgtc acacatttca atgttaaagt
     2941 ggtcggcttt ccctctgaaa catgccacgg gtaacaccat gagtgaatat atcgaagaaa
     3001 accgcggcgc ggacgccatt acccgaccga actggtcagc cgttttctcg gtggcgtttt
     3061 gcgtcgcctg tctgattatc gttgagtttt tgcccgtcag tttgttgacg ccaatggccc
     3121 aggatttagg catttcggaa ggggttgccg ggcaatcggt gaccgtgacc gcctttgtgg
     3181 cgatgtttgc cagtttgttt attacccaga ctattcaggc cactgaccgc cgctacgttg
     3241 tcattctgtt cgccgttttg ctgacgcttt cctgtttgct ggtttccttt gccaactcat
     3301 tcagtctgct gttaatcggt cgcgcctgtc tggggctggc gctgggcggg ttctgggcga
     3361 tgtcggcgtc gctgaccatg cgtctggtac cgccgcgtac ggtgccgaag gcgctgtcgg
     3421 tgatcttcgg cgcggtttct attgcgctgg tgattgctgc gccgttgggc agttttttag
     3481 gcgagcttat cggttggcgc aatgtcttta atgcggcggc ggcgatgggt gtgctgtgta
     3541 ttttctggat tatcaaatca ctgccttcgc tgccaggtga accttcgcat cagaaacaaa
     3601 atactttccg cttattacaa cgtccgggag tgatggccgg gatgatcgcc atctttatgt
     3661 cctttgccgg acagtttgct tttttcactt atattcgccc agtgtatatg aatctggcgg
     3721 gattcggtgt tgatggctta accctggtgc tgctgagttt tggtatcgcc agctttgtcg
     3781 ggacatcgct ttcgtcgttt attctcaaac gttcggtgaa actggcctta gcgggcgcac
     3841 cgtttgtgct cgccctgagc gcgctggtac tgacactgtg gggaagcgat aaaattgttg
     3901 ctactggtgt ggcgattatc tgggggctga cctttgcttt aattcccgtt ggttggtcaa
     3961 cgtggatcac tcgctcattg gccgatcagg cagaaaaagc gggatctatt caggtggccg
     4021 ttattcagct tgctaacacc tgcggtgcgg caatcggcgg ttatgcgttg gataacattg
     4081 gtctgacttc gccgctgatg ttgtccggca cattgatgtt gctgactgca ttgttggtga
     4141 ctgcaaaggt gaaaatgaag aaatcctgat atcaggtaaa gaagcgttgg tcagtggttg
     4201 ttgccgttct tacatatccg ggcattctac cttacccaac gcttcccaat aattgctctg
     4261 attattacgt tccatcgcca cgatggcctc gcgtatatgt tgtttattgt tttcatctga
     4321 caacattaac ttttcccacg cttcatctga taatgacgcc tgtggcgtgc aaattttacg
     4381 taaatcttgc aataccgtgg ttttctcttt tgccgactgt agagaactga atggcgactc
     4441 ggcctgagaa atggctggaa aaatcaggaa tacggtaatc atgagtagca tcgttggctt
     4501 catagcggca accttctgca aatgtgcatc gaatgaagca taagtgtagt ttgcttttgc
     4561 cgctctcatc atgctgtacg aaaggtgttt gcgtggtcag taagaagtgc cagactttat
     4621 attccacttt tattcctttt tattcttatc gatagcgttt cgttttttat cagctatctt
     4681 tccgagataa gaaccaataa ataattacct ttattctatt gc
//
LOCUS       DADSYM010000211         4706 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2415
            SAMN08039579-rid11723173.denovo.213, whole genome shotgun sequence.
ACCESSION   DADSYM010000211 DADSYM010000000
VERSION     DADSYM010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039579
            Sequence Read Archive: SRR6362533
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4706)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4706)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980162.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 03:39:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,484
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 4,347
            CDSs (with protein)               :: 4,347
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 43 of 85
            Pseudo Genes (incomplete)         :: 37 of 85
            Pseudo Genes (internal stop)      :: 19 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4706
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039579-rid11723173.denovo.213"
                     /strain="LGE2415"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8302 N 95.9628 W"
                     /collection_date="2015-08-24"
     gene            complement(39..800)
                     /gene="kduD"
                     /locus_tag="J1Y83_004111"
     CDS             complement(39..800)
                     /gene="kduD"
                     /locus_tag="J1Y83_004111"
                     /EC_number="1.1.1.127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxy-D-gluconate 5-dehydrogenase
                     KduD"
                     /protein_id="HBA7632202.1"
                     /translation="MILSAFSLEGKVAVVTGCDTGLGQGMALGLAQAGCDIVGINIVE
                     PTETIKQVTALGRRFLSLTADLRKIDGIPALLDRAVAEFGHIDILVNNAGLIRREDAL
                     EFSEKDWDDVMNLNIKSVFFMSQAAAKHFIAQGNGGKIINIASMLSFQGGIRVPSYTA
                     SKSGVMGVTRLMANEWAKHNINVNAIAPGYMATNNTQQLRADEQRSAEILDRIPAGRW
                     GLPSDLMGPVVFLASSASDYVNGYTIAVDGGWLAR"
     gene            complement(830..1669)
                     /gene="kduI"
                     /locus_tag="J1Y83_004112"
     CDS             complement(830..1669)
                     /gene="kduI"
                     /locus_tag="J1Y83_004112"
                     /EC_number="5.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
                     /protein_id="HBA7632203.1"
                     /translation="MDVRQSIHSAHAKTLDTQGLRNEFLVEKVFVADEYTMVYSHIDR
                     IIVGGIMPVTKTVSVGGEVGKQLGVSYFLERRELGVINIGGAGTITVDGQCYEIGHRD
                     ALYVGKGAKEVVFASIDTATPAKFYYNCAPAHTTYPTKKVTPDEVSPVTLGDNLTSNR
                     RTINKYFVPDVLETCQLSMGLTELAPGNLWNTMPCHTHERRMEVYFYFNMDDDDACVF
                     HMMGQPQETRHIVMHNEQAVISPSWSIHSGVGTKAYTFIWGMVGENQVFDDMDHVAVK
                     DLR"
     gene            complement(1956..3137)
                     /locus_tag="J1Y83_004113"
     CDS             complement(1956..3137)
                     /locus_tag="J1Y83_004113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HBA7632204.1"
                     /translation="MKDVVIVGALRTPIGCFRGALAGHSAVELGSLVVKALIERTGVP
                     AYAVDEVILGQVLTAGAGQNPARQSAIKGGLPNSVSAITINDVCGSGLKALHLATQAI
                     QCGEADIVIAGGQENMSRAPHVLTDSRTGAQLGNSQLVDSLVHDGLWDAFNDYHIGVT
                     AENLAREYGISRQLQDAYALSSQQKARAAIDAGRFKDEIVPVITQSNGQTLVVDTDEQ
                     PRTDTSAEALARLNPSFDSLGSVTAGNASSINDGAAAVMMMSEAKARALNLPVLARIR
                     AFASVGVDPALMGIAPVYATRRCLERVGWQLADVDLIEANEAFAAQALSVGKMLEWDE
                     RRVNVNGGAIALGHPIGASGCRILVSLVHEMVKRNARKGLATLCIGGGQGVALTIERD
                     E"
     gene            3392..4621
                     /locus_tag="J1Y83_004114"
     CDS             3392..4621
                     /locus_tag="J1Y83_004114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAAAP family serine/threonine permease"
                     /protein_id="HBA7632205.1"
                     /translation="MSNIWSKEETLWSFALYGTAVGAGTLFLPIQLGSAGAVVLFITA
                     LVAWPLTYWPHKALCQFILSSKTSAGEGITGAVTHYYGKKIGNLITTLYFIAFFVVVL
                     IYAVAITNSLTEQLAKHMVIDLRIRMLVSLGVVLILNLIFLMGRHATIRVMGFLVFPL
                     IAYFLFLSIYLVGSWQPDLLTTQVEFNQNTLHQIWISIPVMVFAFSHTPIISTFAIDR
                     REKYGEHAMDKCKKIMKVAYLIICISVLFFVFSCLLSIPPSYIEAAKEEGVTILSALS
                     MLPNAPAWLSISGIIVAVVAMSKSFLGTYFGVIEGATEVVKTTLQQVGVKKSRAFNRA
                     LSIMLVSLITFIVCCINPNAISMIYAISGPLIAMILFIMPTLSTYLIPALKPWRSIGN
                     LITLIVGILCVSVMFFS"
BASE COUNT         1215 a         1201 c         1046 g         1244 t
ORIGIN      
        1 ctgccatgcg acagggtcat aaaagtaaga ggaatgaatt aacgcgccag ccaaccgcca
       61 tccacggcaa tggtataacc attcacataa tctgaagcgc tggaggcaag gaacaccacc
      121 ggccccatca ggtcactcgg cagtccccaa cggccagctg gaatgcggtc gagaatttcc
      181 gcgctacgtt gttcatctgc ccgcagttgt tgagtattgt tggtcgccat gtaacccgga
      241 gctatcgcat taacattaat gttgtgttta gcccattcgt tcgccatcaa tcgcgtcacg
      301 cccatcacac cgctttttga tgcggtataa gaaggcacac ggatcccgcc ctggaaggag
      361 agcattgacg cgatattgat aatcttgccg ccattgcctt gcgcgataaa gtgtttcgcc
      421 gctgcctgag acatgaagaa tacgctcttg atattcaggt tcatgacatc gtcccagtct
      481 ttttcgctga attcgagagc gtcttcgcgg cgaatcaatc cggcgttatt caccaggata
      541 tcaatatgac caaactcagc taccgcgcga tccagcagtg ctggaatgcc atcaatcttt
      601 cgcagatcgg cggtcaggct taaaaaacga cgccccagcg ccgtgacctg cttgatggtt
      661 tcagtcggtt caacgatgtt aatgccaaca atgtcacagc ccgcttgcgc cagccccaac
      721 gccatcccct ggcccagccc agtatcacaa ccagtgacga ccgcaacttt accttcgaga
      781 gaaaatgcac ttaaaatcat aacaatacct tattcgttta tgcccacaac tagcgcaaat
      841 ctttaacggc cacatggtcc atatcatcaa aaacctggtt ttcaccgacc atgccccaga
      901 taaaggtata agctttggtt ccgacgccgg aatggatcga ccagctcggg gagatcaccg
      961 cctgctcgtt atgcatcaca atgtgacgcg tttcttgcgg ttgccccatc atgtggaaaa
     1021 cgcaggcgtc atcatcatcc atattgaaat agaaataaac ttccatccgg cgctcgtggg
     1081 tgtgacacgg catggtgttc cacaagttac ctggagccag ctccgtcagc cccatactca
     1141 attggcaggt ttccagcaca tccgggacaa aatatttgtt aatcgtgcga cggttactgg
     1201 tgaggttatc gcctaacgtg actggagata cttcgtccgg tgtgactttt ttggtgggat
     1261 acgtcgtatg cgcgggtgcg caattgtaat aaaacttcgc cggagtggcg gtatcaatac
     1321 tggcaaagac aacttctttt gcacctttac caacatacag ggcgtcgcgg tgaccgattt
     1381 catagcattg accatcgaca gtaatcgtac cggcaccgcc aatattgata acacctaact
     1441 cgcgacgttc aaggaaatag cttacgccga gttgtttacc aacttccccg ccaacggaaa
     1501 ccgttttagt taccggcata atgccgccaa caataatacg gtcaatgtgg ctgtaaacca
     1561 tggtgtactc atcggcgaca aatacctttt caaccaaaaa ttcattgcgc agcccctggg
     1621 tatccagcgt ttttgcgtgc gcactgtgga tgctctgtct tacgtccaca tcaacctccg
     1681 ataatcagtg aagcgtgaaa atagaaaaca acaatgaaac actgtttcgt tttctctatg
     1741 aggggatgat atgcccatgg gatagggttt tcaattaaaa ataaaacaat gtttcatttt
     1801 caagttaagg attaaaaaaa gtgcagtgtc gatcacgaat atgtcatgag aatggaaaaa
     1861 tcagggagcc ctgaaaaggt atgtcgactc accagccatc attattcaac agattaatga
     1921 cggcagatat ttatcggtaa ggaggaaatg cagagctact cgtcacgttc aatggtcaat
     1981 gccacaccct ggcctccgcc gatacaaagc gttgccagtc ctttgcgggc attacgtttc
     2041 accatttcat gaacaagaga aaccaggatt cggcaaccgg aagcgcctat cgggtgaccg
     2101 agtgcgatcg caccaccatt gacattgacc cgacgctcat cccattcaag catcttgcca
     2161 accgaaagcg cctgtgcagc aaacgcttca ttagcctcga taagatcgac atcagccaac
     2221 tgccagccaa cacgctccag gcaacggcgg gtcgcataca ccggcgcaat tcccatcaat
     2281 gccggatcca caccaacgct ggcaaatgcg cggatgcggg ccagcacggg taaattcaac
     2341 gctcgtgctt tggcttcgct catcatcatt actgcagctg ccccatcgtt tatggatgat
     2401 gcattacccg ctgtcacaga accgagacta tcaaatgaag gatttaaacg ggctaaggct
     2461 tctgcgctgg tgtcagtgcg tggctgttca tcggtatcaa caaccaacgt ctgaccgtta
     2521 ctttgggtta ttaccgggac gatctcatct ttaaatcgtc cggcgtcaat cgccgctcgc
     2581 gctttttgtt gcgagctaag tgcgtaagca tcctgcaact gacggctgat gccatattcg
     2641 cgagccagat tttcggcggt gacaccaata tgataatcat tgaaggcatc ccacaaccca
     2701 tcatgcacaa gactatcaac caactggcta ttgccaagct gtgcgccggt gcggctatca
     2761 gtcagaacat gtggtgcgcg gctcatgttt tcctggccac cggcgatgac aatatcagcc
     2821 tcgccacact gtatcgcctg agtagccaga tgcagtgctt taagcccgga accacaaacg
     2881 tcattaatag tgattgcaga aacgctatta ggaagaccac ctttaatagc cgattgcctt
     2941 gccggattct gccctgcccc tgcagtcaac acctgaccaa gaatcacttc atccaccgca
     3001 tatgcaggaa cgccggtacg ttctattaac gctttgacga ccaggctgcc aagttccacg
     3061 gcggaatgac ccgctaacgc accacgaaag cagccgatag gtgtccgtaa cgccccgaca
     3121 atcacaacgt ctttcatcac aacctcgcac caattaacag tgcaatagta aatgattgtt
     3181 aattacagtt atcttaattg tttaaaaaag gtgattttta tcacaaagga aatacgcctg
     3241 agtagcagtc agagacataa ctggcacgta aggtttgcaa ccactaactc accaatagag
     3301 aggtagatag ggcgttaatc tcccatactt aacctggttt atggtaaatt gccctccatt
     3361 ttgtttaatt tgtagatgat acgttcagat aatgtctaat atttggtcaa aagaagaaac
     3421 tctgtggagt ttcgcgctct acggcacagc cgttggtgca ggcacgctct tccttcctat
     3481 tcagttaggt tcggcggggg ctgtggtcct gtttattact gctctggtcg cctggccttt
     3541 aacatattgg ccacataaag ccttatgcca gttcatcctc tcatcgaaaa catcagcagg
     3601 tgaagggata acgggcgcgg taacacacta ctatggcaag aagattggta atctgattac
     3661 cacgctgtac ttcatcgcct tttttgtcgt cgtgttgata tatgcagtgg caattaccaa
     3721 ctcacttacg gaacagctgg caaagcatat ggttattgat cttcgcatcc gtatgttggt
     3781 gagtctgggg gttgtattaa tcctgaatct catttttctg atgggacgcc atgcaaccat
     3841 tcgggtaatg ggatttttgg tattcccatt gattgcctat ttcttatttc tttctattta
     3901 cctggtcggt agttggcaac ctgatctatt aacaacccag gtagagttca atcagaatac
     3961 ccttcaccag atatggatat cgattcccgt gatggttttc gcttttagcc atacgcccat
     4021 tatttctacg tttgccatag acagacgtga aaaatatggc gaacatgcta tggataaatg
     4081 caaaaaaatt atgaaagtcg cttatctcat catctgcata agtgtattgt tctttgtctt
     4141 tagctgcctg ctttctattc caccttcgta tattgaagcg gctaaagaag aaggggttac
     4201 cattttatcg gcgctttcta tgctgccgaa cgccccagca tggttgtcaa tttccgggat
     4261 tattgtcgca gtagttgcga tgtcgaaatc attcctgggt acgtactttg gcgttattga
     4321 aggtgccaca gaggtcgtca aaacaacatt acagcaggtt ggtgtaaaga aaagtcgtgc
     4381 atttaaccgc gcactatcaa ttatgttggt atcgctgatt accttcattg tttgttgcat
     4441 taacccgaac gcgatttcga tgatttacgc gatcagcggc ccgctcattg ccatgatact
     4501 tttcatcatg cctacgctgt cgacgtatct catcccggcg cttaaaccct ggcgttccat
     4561 cggaaatctg attaccctga tcgtgggtat cctgtgcgta tcggtaatgt tctttagcta
     4621 atatccgttg tggatggttc aaaattgctt caattttgaa ccatcccagt ttttataatg
     4681 ttcatttgtt attattccgc tctatc
//
LOCUS       DADSYM010000212         4695 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2415
            SAMN08039579-rid11723173.denovo.214, whole genome shotgun sequence.
ACCESSION   DADSYM010000212 DADSYM010000000
VERSION     DADSYM010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039579
            Sequence Read Archive: SRR6362533
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4695)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4695)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980162.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 03:39:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,484
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 4,347
            CDSs (with protein)               :: 4,347
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 43 of 85
            Pseudo Genes (incomplete)         :: 37 of 85
            Pseudo Genes (internal stop)      :: 19 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4695
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039579-rid11723173.denovo.214"
                     /strain="LGE2415"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8302 N 95.9628 W"
                     /collection_date="2015-08-24"
     gene            86..1060
                     /gene="hemB"
                     /locus_tag="J1Y83_004115"
     CDS             86..1060
                     /gene="hemB"
                     /locus_tag="J1Y83_004115"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HBA7632206.1"
                     /translation="MTDLIQRPRRLRKSPALRAMFEETTLSLNDLVLPIFVEEEIDDY
                     KAVEAMPGVMRIPEKHLAREIERIANAGIRSVMTFGISHHTDETGSDAWREDGLVARM
                     SRICKQTVPEMIVMSDTCFCEYTSHGHCGVLCEHGVDNDATLENLGKQAVVAAAAGAD
                     FIAPSAAMDGQVQAIRQALDAAGFKDTAIMSYSTKFASSFYGPFREAAGSALKGDRKS
                     YQMNPMNRREAIRESLLDEAQGADCLMVKPAGAYLDIVRELRERTELPIGAYQVSGEY
                     AMIKFAALAGAIDEEKVVLESLGSIKRAGADLIFSYFALDLAEKKILR"
     gene            complement(1100..1951)
                     /gene="tauD"
                     /locus_tag="J1Y83_004116"
     CDS             complement(1100..1951)
                     /gene="tauD"
                     /locus_tag="J1Y83_004116"
                     /EC_number="1.14.11.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="taurine dioxygenase"
                     /protein_id="HBA7632207.1"
                     /translation="MSERLSITPLGPYIGAQITGADLTRPLSDNQFEQLYHAVLRHQV
                     VFLRDQAITPQQQRALAQRFGELHIHPVYPHAEGVDEIIVLDTHNDNPPDNDNWHTDV
                     TFIQTPPAGAILAAKELPSTGGDTLWASGIAAYEALSVPFRQLLRGLRAEHDFRKSFP
                     EYKYRKTEAEHQRWREAVAKNPPLLHPVVRTHPVTGKQALFVNEGFTTRIVDVSEKES
                     EALLGFLFAHITKPEFQVRWRWQPNDIAIWDNRVTQHYANADYLPQRRIMHRATILGD
                     IPFYRAG"
     gene            complement(1948..2775)
                     /gene="tauC"
                     /locus_tag="J1Y83_004117"
     CDS             complement(1948..2775)
                     /gene="tauC"
                     /locus_tag="J1Y83_004117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter permease TauC"
                     /protein_id="HBA7632208.1"
                     /translation="MSVLINEKLHSRRLKWRWPLSRQVTLSIGTLAVLLTVWWAVAAL
                     QLISPLFLPPPQQVLAKLLTIAGPQGFMDATLWQHLAASLTRILLALLAAVLIGIPVG
                     IAMGLSPTVRGILDPIIELYRPVPPLAYLPLMVIWFGIGETSKILLIYLAIFAPVAMS
                     ALAGVKSAQQVRIRAAQSLGASRAQVLWFVILPGALPEILTGLRIGLGVGWSTLVAAE
                     LIAATRGLGFMVQSAGEFLATDVVLAGIAVIAIIAFLLELGLRALQRRLTPWHGEVQ"
     gene            complement(2772..3539)
                     /gene="tauB"
                     /locus_tag="J1Y83_004118"
     CDS             complement(2772..3539)
                     /gene="tauB"
                     /locus_tag="J1Y83_004118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter ATP-binding subunit"
                     /protein_id="HBA7632209.1"
                     /translation="MLQISHLYADYGGKPALEDINLTLESGELLVVLGPSGCGKTTLL
                     NLIAGFVPYQHGSIQLAGKGIQGPGAERGVVFQNEGLLPWRNVQDNVAFGLQLAGVEK
                     MQRLEIAHQMVKKVGLEGAEKRYIWQLSGGQRQRVGIARALAANPQLLLLDEPFGALD
                     AFTRDQMQTLLLKLWQETGKQVLLITHDIEEAVFMATELVLLSPGPGRVLERLPLNFA
                     RRFVAGEPSRSIKSDPQFIAMREYVLSRVFEQREAFS"
     gene            complement(3552..4514)
                     /gene="tauA"
                     /locus_tag="J1Y83_004119"
     CDS             complement(3552..4514)
                     /gene="tauA"
                     /locus_tag="J1Y83_004119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter substrate-binding
                     protein"
                     /protein_id="HBA7632210.1"
                     /translation="MAISSRNTFLAALAFIAFQAQAVNVTVAYQTSAEPAKVAQADNT
                     FAKESGATVDWRKFDSGASIVRALASGDVQIGNLGSSPLAVATSQQVPIEVFLLASKL
                     GNSEALVVKKTISKPEDLIGKRIAVPFISTTHYSLLAALKHWGIKPGQVEIVNLQPPA
                     IIAAWQRGDIDGAYVWAPAVNALEKDGKVLTDSEQVGQWGAPTLDVWVVRKDFAEKHP
                     EVVKAFAKSAIDAQQPYIANPDAWLKQPENISKLARLSGVPEGDIPGLVKGNTYLTPQ
                     QQTAELTGPVNKAIIDTAQFLKEQGKVPAVANDYSQYVTSRFVQ"
BASE COUNT         1083 a         1362 c         1207 g         1043 t
ORIGIN      
        1 tcaacatcgc gacaactttc gtaaaacaca cccagcctgc ttcaggtata ctatgctcct
       61 cgattccaca aacatcaggc agaccatgac agatttaatc caacgccctc gtcgcctgcg
      121 caaatctcct gcgctgcgcg ctatgtttga agagacaaca cttagcctta acgacctggt
      181 gttgccgatc tttgttgaag aagaaattga cgactacaaa gccgttgaag ccatgccagg
      241 cgtgatgcgc attccagaga aacatctggc acgcgaaatt gaacgcatcg ccaacgccgg
      301 tattcgttct gtgatgactt tcggcatctc tcaccatacc gatgaaaccg gcagcgatgc
      361 ctggcgggaa gatggactgg tggcgcgtat gtcgcgcatc tgcaagcaga ccgtgccaga
      421 aatgatcgtt atgtcagaca cctgcttctg tgaatacaca tctcacggtc actgcggtgt
      481 tctgtgcgag catggcgtcg acaacgacgc gactctggaa aatttaggca agcaagctgt
      541 ggttgcagct gctgcaggcg cagacttcat cgccccttct gccgcgatgg atggtcaggt
      601 acaggcgatt cgccaggcgc tggacgctgc gggctttaaa gatacggcga ttatgtcgta
      661 ttcgaccaag ttcgcctcct ccttttatgg cccgttccgt gaagctgccg gaagcgcatt
      721 aaaaggcgac cgcaaaagct atcagatgaa cccaatgaac cgtcgtgagg cgattcgtga
      781 gtcactgctg gatgaagccc agggcgcaga ctgcctgatg gttaaacctg ccggagcata
      841 cctcgacatc gtgcgtgagc tgcgtgaacg tactgaattg ccgattggcg cgtatcaggt
      901 gagcggtgag tacgcgatga ttaagttcgc cgcgctggcg ggtgctatag atgaagagaa
      961 agtcgtgctc gaaagcttag gttcgattaa gcgtgcgggt gcggatctga ttttcagcta
     1021 ctttgcgctg gatttggctg agaagaagat tctgcgttaa tgatcattat gccgggttat
     1081 ccaacccggc atttccccct taccccgccc gataaaacgg aatatcccca agaatcgtcg
     1141 cccgatgcat aatccgccgc tgtggcaggt aatccgcatt ggcatagtgc tgagtcactc
     1201 ggttatccca aatcgcaata tcattcggtt gccagcgcca gcgcacctga aactccggtt
     1261 tagtgatatg ggcaaacaaa aaacctaaca aggcttcgct ctctttctcg ctgacatcaa
     1321 caattcgtgt ggtaaaacct tcgttcacaa acagcgcctg tttaccggtc accggatgcg
     1381 ttcgcaccac cggatgtagc aacggtggat ttttcgcgac cgcctcacgc cagcgttgat
     1441 gctcagcctc ggttttgcgg tacttgtatt ccgggaacga tttacggaaa tcatgctccg
     1501 cacgcaaccc tctcaacagc tggcggaagg gaacagagag cgcctcatat gcggcaatac
     1561 cactggccca gagcgtatcg ccgccggtcg agggtaactc tttcgccgcc agaatcgccc
     1621 ctgcgggtgg cgtttgaata aatgtcacat cggtgtgcca gttgtcgtta tccggcggat
     1681 tatcgttatg ggtatccagg acgatgatct cgtcaacccc ttcagcatga gggtaaacag
     1741 ggtgaatatg cagctcgcca aaacgctggg ccagcgcgcg ttgctgctgc ggcgtaatag
     1801 cttgatcgcg cagaaacacc acctgatggc gcagcaccgc atggtaaagc tgttcaaact
     1861 gattatcgct taacgggcgc gttaagtcgg cccccgtaat ttgtgcgcca atatacggcc
     1921 ccagcggggt aatgctcaga cgttcactca ttgtacttct ccatgccagg gcgtcaggcg
     1981 gcgctgtaac gcgcgcagac ccagttctaa aagaaaggcg ataatcgcaa tcaccgcgat
     2041 ccccgccagc accacgtcag tcgcgagaaa ttcacccgct gactgaacca taaatcctaa
     2101 accgcgcgtt gcggcaatca gctccgccgc caccagcgta gaccagccca cacccagacc
     2161 aatgcgtaat ccggtgagga tttccggcag cgcaccgggc aaaatgacaa accacagcac
     2221 ctgcgcacgg ctggcaccca gcgactgggc ggcacgaatg cgaacctgct gcgcgctttt
     2281 cacccccgcc agcgccgaca tcgccaccgg cgcaaaaatc gctaaataga tcagtaagat
     2341 cttcgaggtt tcgccaatac caaaccagat caccatcagc ggcaaataag ccagcggcgg
     2401 caccggacga taaagctcga ttatcggatc cagaatgccg cgcaccgtcg ggcttagccc
     2461 catggcgatc ccgaccggaa taccgatcaa caccgccgcg agtagcgcca gcaaaatgcg
     2521 cgtcaggctg gctgccagat gctgccacag cgtggcgtcc ataaatccct gcggcccggc
     2581 aatggtgagc agttttgcca gtacctgttg cggtggcggc aaaaacagcg ggctaatcag
     2641 ttgcagcgcc gcgactgccc accatacagt gagcaaaacc gctaacgtac caatgcttaa
     2701 ggtcacctga cgcgagagcg gccagcgcca tttcagccga cgcgaatgca gtttttcatt
     2761 aataagcaca ctcatgagaa cgcctcccgt tgctcaaata cgcggcttaa aacatattcg
     2821 cgcatggcga tgaattgtgg atcggacttg atgctgcggc tcggttcccc ggcaacaaag
     2881 cggcgggcga agttgagcgg cagccgctcc agcacgcggc ctgggccggg tgaaagcaga
     2941 accagttcag tcgccataaa caccgcttct tctatatcgt gggtaatcaa cagcacctgc
     3001 tttcccgtct cctgccaaag tttcagcagc agggtttgca tctggtcgcg ggtgaaggcg
     3061 tccagcgcac caaacggttc gtcgagtaat aacagctggg gatttgccgc caacgcgcgg
     3121 gcaatcccca cccgctgacg ttgaccaccg gaaagctgcc agatgtagcg tttttctgcg
     3181 ccttccagcc cgactttttt caccatctgg tgtgcgattt ccagtcgctg cattttctct
     3241 acacctgcca gttgcaggcc gaacgccacg ttgtcctgta cattgcgcca cggtaacagc
     3301 ccttcattct gaaaaactac gccacgctct gctcccggtc cctgaatacc cttacccgcc
     3361 agttgaatgc tgccatgctg ataaggcaca aaaccagcaa tcaaattcag caacgtagtt
     3421 ttgccgcagc cagacggccc cagaactacc agaagctcgc cgctttccag cgtcaggttg
     3481 atatcttcca gtgccggttt gccgccataa tcggcgtaaa gatgagaaat ttgcagcatc
     3541 tgcgcctcct tttattgcac gaagcgcgag gtaacgtact ggctgtaatc attcgctaca
     3601 gccgggactt tgccctgctc tttcaaaaac tgcgcggtgt cgatgatcgc tttgttcacc
     3661 ggtccggtca gttctgccgt ttgctgctgc ggcgtcagat aggtattccc cttcaccagc
     3721 cccggaatgt caccttccgg cacgccgctt aaccgcgcca gtttgctgat gttttccggc
     3781 tgtttcagcc acgcgtctgg gttagcaatg tacggttgct gagcatcgat ggcgctttta
     3841 gcgaacgctt tcacgacctc aggatgtttc tcggcaaaat ctttccgcac gacccagacg
     3901 tccagtgtcg gcgcgcccca ctgcccgacc tgttcagaat cggtcagcac cttgccatct
     3961 ttttccaggg cgttaaccgc cggtgcccag acataagcac catcaatatc tccccgctgc
     4021 catgcggcga taatcgcggg cggctgcagg ttcacaatct caacttgccc aggtttaata
     4081 ccccagtgtt tcagcgccgc cagcaagctg tagtgggtgg tggagataaa cggtacggcg
     4141 atgcgtttac caatcaagtc ttccggttta ctgatagttt tctttaccac cagcgcttcg
     4201 gagttaccca gttttgacgc cagcaagaag acttcaatcg gcacctgttg gctggttgca
     4261 accgctaacg ggctggaacc gaggttgccg atttgcacgt cgcccgaagc cagcgcccgc
     4321 acgatgctgg ctccactgtc aaacttacgc cagtccacgg ttgctccgct ttctttagca
     4381 aaggtgttgt cggcctgagc cactttcgcc ggttcggcgg aggtttgata cgccacggtg
     4441 acgttcaccg cttgcgcctg aaaagcgatg aatgccagtg cggcaagaaa tgtgttacgc
     4501 gatgaaattg ccatgattgt ctgctccccc gtcttgttat gggagcagta ttcaggaata
     4561 aaaacattca ttaaaagaat tagtcgttat cgtacagatg attttattct tagcaaaaaa
     4621 gcggcaaagg aaagattaaa tatgcaatta cgcccactct gataactcag taaattactg
     4681 cacttcattt tatta
//
LOCUS       DADSYM010000213         4660 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2415
            SAMN08039579-rid11723173.denovo.215, whole genome shotgun sequence.
ACCESSION   DADSYM010000213 DADSYM010000000
VERSION     DADSYM010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039579
            Sequence Read Archive: SRR6362533
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4660)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4660)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980162.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 03:39:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,484
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 4,347
            CDSs (with protein)               :: 4,347
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 43 of 85
            Pseudo Genes (incomplete)         :: 37 of 85
            Pseudo Genes (internal stop)      :: 19 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4660
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039579-rid11723173.denovo.215"
                     /strain="LGE2415"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8302 N 95.9628 W"
                     /collection_date="2015-08-24"
     gene            135..554
                     /locus_tag="J1Y83_004120"
     CDS             135..554
                     /locus_tag="J1Y83_004120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008323638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="entry exclusion protein 1"
                     /protein_id="HBA7632211.1"
                     /translation="MAWHTLRDALKMTGRSRSQFYRDMRAGLVSYRTSQDGRREFETS
                     ELIRAYGELKRNETPERHTAGQAEKPHDQQTERILRELSELKQCLTLMLEDKQAQDTE
                     RRRQEAEREQLQNEIAQLRQALEQERKRGFWSRLFGR"
     gene            635..1063
                     /gene="exc2"
                     /locus_tag="J1Y83_004121"
     CDS             635..1063
                     /gene="exc2"
                     /locus_tag="J1Y83_004121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000830439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Exc2 family lipoprotein"
                     /protein_id="HBA7632212.1"
                     /translation="MKRSGGTRYMYLSALITVSAALTACTPKTSLEQHTRHYVYASDD
                     GFDPNFYTKKADTTRMMIPFFQQFREMGVKDKAAGVSAETAQQRIKEFHSEEFLQSLR
                     TTTTFAGRKYTNSDMPSPEKMKLLADTISAIYLDGYEGRQ"
     gene            1894..2085
                     /locus_tag="J1Y83_004122"
     CDS             1894..2085
                     /locus_tag="J1Y83_004122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="HBA7632213.1"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDH
                     ADELYRSCLARFGDDGETL"
     gene            complement(3060..3191)
                     /locus_tag="J1Y83_004123"
     CDS             complement(3060..3191)
                     /locus_tag="J1Y83_004123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001368419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="HBA7632214.1"
                     /translation="MRGLVFCALILILPGCQANYIRDVQGGTVAPSSSSAATGIEIR"
     gene            complement(3203..3682)
                     /locus_tag="J1Y83_004124"
     CDS             complement(3203..3682)
                     /locus_tag="J1Y83_004124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001485345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA7632215.1"
                     /translation="MIKRLLFLYVALLFLSEGAYASVTVFIAPSWERNTDSVNEIYKY
                     IDDINSNNHVIDQAYVEPIRKELGVYRDYIQKKLINFNGKGVCKLSITTGSTGVKDIE
                     PPDVVLLNSLAENSSLDCKKLYREIQSIVDNDPSLLFPEKIKSNGLLSIDMIIVMGR"
     gene            complement(3765..4523)
                     /locus_tag="J1Y83_004125"
     CDS             complement(3765..4523)
                     /locus_tag="J1Y83_004125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001555316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA7632216.1"
                     /translation="MSGIENSNQGPGWHDHNTPYGTIHSYDGDSHSGYGAHSGGNGGN
                     RHEGISDKTHISIPSTRNYNGYYMSMCVNNLKAHEGFKNTMYKDSEGNITVGIGHLLS
                     TPDMAASLPFTRTHTWHGHGDDMEKEVAASKKEITDAFNNFKKDSRQVPMNLHLSNDA
                     VIGLCISDVQTTEKGLRSLYSGYDGFSNSRKTALVDMGFNLGIPKLSRNFPNFNAAVN
                     RGDWESAARESHRNLHQRGDQRNKDTADQLRDKH"
BASE COUNT         1330 a         1128 c          999 g         1203 t
ORIGIN      
        1 aaaaaaacag ccactcacac catgtgccat ccccgccaga tgccacaaaa accagcacaa
       61 acattaccgt tctccggcct cagtatgttt tactgagacc atgagatgag acacctatgg
      121 gacactgtca ctttatggca tggcacacac tccgggacgc actgaaaatg acgggcagat
      181 cgcgttcaca gttttaccgt gatatgcgcg caggtcttgt cagttaccgt acaagccagg
      241 acggacgacg ggagtttgaa actagtgaac tgatccgggc atacggcgaa ttaaagcgga
      301 atgagacacc agaaaggcac acagcgggac aggcagaaaa acctcatgat cagcagacag
      361 aacgcattct ccgggagctg agcgagctga aacaatgcct gacgctgatg cttgaggata
      421 aacaagccca ggatacggag cgccggcgcc aggaagcaga acgggaacaa ctacagaatg
      481 agatagccca gctcaggcag gcgctggagc aggaaaggaa acggggattc tggtcccggt
      541 tgtttggtcg ctgaacgctg tcagagactg atgataagat agtcttctga taataactca
      601 ccgaaaataa atacattaag gtaaggagac tcgcatgaaa cgttctggcg gcacacgcta
      661 tatgtatctt agcgccctga taactgtgtc agcagccctc acagcctgta ccccaaaaac
      721 cagcctggaa caacataccc ggcattatgt ttatgcttca gatgatgggt tcgatcctaa
      781 cttctacacc aagaaagctg acaccacacg gatgatgatt ccgttctttc agcagttcag
      841 ggagatgggg gtgaaagaca aagcagccgg agtatcagca gaaacggcac agcaacgcat
      901 aaaagaattc cactctgaag aattccttca gtcactgaga accacaacca cctttgcggg
      961 cagaaaatac acgaacagtg atatgccatc accggaaaaa atgaagctgc tggctgacac
     1021 gatttctgcg atttatctcg atggctacga gggaagacag taagtggatt taccataatc
     1081 ccttaattgt acgcaccatt aaaacgcgtt gagcgcgatt ctgacgcagg cacagcaaaa
     1141 agaaatgttt acccccagga aaaccggaaa aatgcgtcag gatcgttttc agggcgttgc
     1201 atgactatgc acgaaactga atagcgatct gtttgggcgc atacggtgcc catgaggcac
     1261 attttcggac attttcagtt cgtgaatccc tggctcattg ctacatatcc gggcagacac
     1321 ccctggcagc gcccccaggg gtgttatgta aaaaaaggcg acgcgcccct aggggcgccc
     1381 ctacattaat taactgtttt ttctacagat agtcagtact gaccctaagt agggtcgtca
     1441 tagcgtcgcc atagggtcgt cgacgacgtg cttttgtata aatctgaata agaaaataca
     1501 tttttaaaac cataacaaca tgatttaaaa agcaaatcag caaaaagttg gttttgcgtg
     1561 gggtgtggac atttcagaca tgagaacaga ctcgcgtttt tccggagtat cagcggaata
     1621 tctgattaaa aaacactcgc cgcagagcgc gattttatgc aacagctgct gtactgcaca
     1681 gggattattt atcagttgat atgctgtgcc atgatattgc tattgccgaa agggacgtcg
     1741 ttttacggcg ttttccctat cctgcatcgc actggccgcg ttcgaatgtt tgcgcccctg
     1801 actaaccgct cactcagctt tatcactctc gcctgcgtca cagttatcat gctcccatga
     1861 acagaaattc ccttacacgg agacatcaga ccagtgacca aacaggaaaa aaccgccctg
     1921 aacatggccc gcttcatcag aagccagaca ttaaccctgc tggagaagct caacgaactg
     1981 gacgcggatg aacaggcaga catctgtgaa tcgcttcacg accacgccga tgagctttac
     2041 cgcagttgcc tcgcacgttt cggggatgac ggtgaaaccc tctgacatat gcagctcccg
     2101 gagacggtca cagcttgtct gtgagcgaat gccgggagca gacaagcccg tcagggcgcg
     2161 tcagcgggtt ttagcgggtg tcggggcgca gccatgaccc agtcacgtag cgatagcgga
     2221 gtgtatactg gcttaatcat gcggcatgtc cggtgtgaaa tgccgcacag atgcgtaagg
     2281 agaaaatgcc agtataggcg ctcttccgct tcctcgctca ctgactcgct acgctcggtc
     2341 gttcggctgc ggcgagcggt gtctactcac tcaaaagctg taatacagtt atccacagaa
     2401 tcaggggata atgccggaaa gaacatgtga gcaaaaagga aaaaggccgc gcagctgacg
     2461 tttttccata ggctccgccc ccctgacgag catcacaaaa attgacgctc aagtcagagg
     2521 tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg
     2581 cgctctcctg ttccgaccct gccacttacc ggatacctgt ccgcctttct cccttcggga
     2641 agcgtggcgc tttctcatag ctcacgctgt tggtatctcg gcttggtgta ggtcgttcgc
     2701 tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt
     2761 aactatcgtc ttgagtccaa cccggtaaga cacgacttaa cgccactggc agcagccact
     2821 ggtagaggat gtacaagaaa ttgctgatac gtagttcttg aagtgaagga ccgactgcgg
     2881 ctacactgaa aggacagatt tggtggcagc ggtcttgtac accctgttac ctcgggttag
     2941 aaagcctgag aaacttctaa ccttcgaaag aacccacgcc tgagaacgtg ggttttttcg
     3001 tttacaggca gcagattacg cgtagaaaaa aaggatctca agaagatcct ttaatctttt
     3061 tacctaattt cgattccagt agctgctgag ctagaagatg gcgcaacagt ccctccttga
     3121 acatccctga tataatttgc ctgacagccg ggcaatatta aaattagcgc acaaaaaacc
     3181 aaaccacgca tagaattgta ttttatctcc ccataacaat gatcatatca attgataaca
     3241 aaccatttga ctttattttc tcaggaaata aaagtgacgg atcgttatca acaatacttt
     3301 gtatttctct atacagtttt ttacagtcta atgatgagtt ttccgccaat gagtttaaca
     3361 aaacaacatc tggaggctca atatctttaa cacctgtact tcctgttgtt atcgataatt
     3421 tacaaactcc cttcccatta aagttaatta gttttttctg gatataatct cgatataccc
     3481 ctaactcctt cctaataggt tctacatatg cttgatcgat gacatggtta ttactattta
     3541 tatcatcaat atatttatat atctcattaa ctgaatctgt attcctttcc caacttggag
     3601 caatgaatac tgttacagat gcataagcac cttctgataa gaacaataaa gcaacatata
     3661 agaataataa ccgcttgatc atataatcca ttcccattat aggttgatgt atttcatctt
     3721 taatataaga tgaaatacat ctcatatcta tatgttatgg tctattaatg tttatcccgc
     3781 aactgatctg ctgtatcctt gtttctctga tcgcctcgtt gatgaagatt tcgatggctt
     3841 tcacgtgctg cagactccca atccccacgg ttcacagcag cattaaagtt cggaaagttt
     3901 ctcgatagct taggaattcc taagttaaat cccatatcca ctaaggcagt ttttctagag
     3961 ttagaaaaac catcataacc agaatataaa gaacgcaagc ctttctctgt agtctgaaca
     4021 tcggaaatac ataatccaat aacagcatca tttgataaat gcaagttcat tggtacttgt
     4081 ctagagtctt ttttaaagtt attaaaagca tcggttattt ctttttttga tgctgcaact
     4141 tctttttcca tatcatcacc atgaccatgc catgtatgag ttcttgtaaa agggagagat
     4201 gccgccatat ctggcgtaga tagcaaatga ccaataccaa cagtaatatt accttcgcta
     4261 tccttataca tggtgttttt gaacccctca tgggctttta aattattaac acacatgctc
     4321 atatagtagc cattatagtt tctggtagat ggtatactaa tatgtgtctt atcagaaata
     4381 ccttcatggc gatttccccc attaccacca gaatgagcac cataaccgct atggctgtcc
     4441 ccatcataac tatgaattgt tccataggga gtattgtgat catgccaccc cggaccttga
     4501 ttagaatttt caataccact catcttaatt tcctctttga catttaaaac aacgatcaat
     4561 acaactgtat atataaccac tggttttata tacagtttaa aattacgccg ctgcgttttc
     4621 cctgtcaacc ctgtggattt tcatttttgt gaaaacggtc
//
LOCUS       DADSYM010000214         4497 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2415
            SAMN08039579-rid11723173.denovo.216, whole genome shotgun sequence.
ACCESSION   DADSYM010000214 DADSYM010000000
VERSION     DADSYM010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039579
            Sequence Read Archive: SRR6362533
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4497)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4497)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980162.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 03:39:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,484
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 4,347
            CDSs (with protein)               :: 4,347
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 43 of 85
            Pseudo Genes (incomplete)         :: 37 of 85
            Pseudo Genes (internal stop)      :: 19 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4497
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039579-rid11723173.denovo.216"
                     /strain="LGE2415"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8302 N 95.9628 W"
                     /collection_date="2015-08-24"
     gene            complement(<1..259)
                     /gene="chbB"
                     /locus_tag="J1Y83_004126"
     CDS             complement(<1..259)
                     /gene="chbB"
                     /locus_tag="J1Y83_004126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIB"
                     /protein_id="HBA7632217.1"
                     /translation="MEKKHIYLFCSAGMSTSLLVSKMRAQAEKYEVPVIIEAFPETLA
                     GEKGQNADVVLLGPQIAYMLPEIQRLLPNKPVEVIDSLLYGK"
     gene            complement(559..897)
                     /gene="osmE"
                     /locus_tag="J1Y83_004127"
     CDS             complement(559..897)
                     /gene="osmE"
                     /locus_tag="J1Y83_004127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotically-inducible lipoprotein OsmE"
                     /protein_id="HBA7632218.1"
                     /translation="MNKNMAGILSAAAVLTMLAGCTAYDRTKDQFVQPVVKDVKKGMS
                     RAQVAQIAGKPSSEVSMIHARGTCQTYILGQRDGKAETYFVALDDTGHVINSGYQTCA
                     EYDTDPQATK"
     gene            1099..1926
                     /gene="nadE"
                     /locus_tag="J1Y83_004128"
     CDS             1099..1926
                     /gene="nadE"
                     /locus_tag="J1Y83_004128"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HBA7632219.1"
                     /translation="MTLQQQIIKALGAKPQINAEEEIRRSIDFLKSYLQTYPFIKSLV
                     LGISGGQDSTLAGKLCQMAINELRQETDNESLQFIAVRLPYGVQADEQDCQDAIAFIQ
                     PDRVLTVNIKGAVLASEQALREAGIELSDFVRGNEKARERMKAQYSIAGMTSGVVVGT
                     DHAAEAITGFFTKYGDGGTDINPLYRLNKRQGKQLLAALGCPEHLYKKAPTADLEDDR
                     PSLPDEVALGVTYDNIDDYLEGKNVPQQVARTIENWYLKTEHKRRPPITVFDDFWKK"
     gene            2156..3043
                     /gene="cho"
                     /locus_tag="J1Y83_004129"
     CDS             2156..3043
                     /gene="cho"
                     /locus_tag="J1Y83_004129"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease Cho"
                     /protein_id="HBA7632220.1"
                     /translation="MVRRLTSPRLEFEAAAIYEYPEHLRSFLNDLPTRPGVYLFHGES
                     DTMPLYIGKSVNIRSRVLSHLRTPDEAAMLRQSRRISWICTAGEIGALLLEARLIKEQ
                     QPLFNKRLRRNRQLCALQLNEKRVDVVYAKEVDFSRAPNLFGLFANRRAALQALQSIA
                     DEQKLCYGLLGLEPLSRGRACFRSALKRCAGACCGKESHEEHALRLRQSLERLRVVCW
                     PWKGAVALKEQHPEMTQYHIIQNWLWLGAVNSLKEATTLIRAPAGFDHDGYKILCKPL
                     LSGNYEITELDPVNDQQAS"
     gene            complement(3003..3578)
                     /gene="ves"
                     /locus_tag="J1Y83_004130"
     CDS             complement(3003..3578)
                     /gene="ves"
                     /locus_tag="J1Y83_004130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310475.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="environmental stress-induced protein Ves"
                     /protein_id="HBA7632221.1"
                     /translation="MEYFDMRKMSVNLWRNAAGETREICTFPPAKRDFYWRASITSIA
                     ANGEFSLFPGMERIVTLLEGGEMFLESADRFNHTLKPLQPFSFAADLVVKAKLTAGQM
                     SMDFNIMTRLDVCKAKVRIAERTFTTFGSRGGVVFVINGAWQLGDKLLTTDQGACWFD
                     GRHTLRLLQPQGKLLFSEINWLAGHSPDQVQ"
     gene            complement(3781..4266)
                     /gene="spy"
                     /locus_tag="J1Y83_004131"
     CDS             complement(3781..4266)
                     /gene="spy"
                     /locus_tag="J1Y83_004131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-independent periplasmic protein-refolding
                     chaperone"
                     /protein_id="HBA7632222.1"
                     /translation="MRKLTALFVASTLALGAANLAHAADTTTAAPADAKPMMHHKGKF
                     GPHEDMMFKDLNLTDAQKQQIREIMKGQRDQMKRPPLEERRAMHDIIASDTFDKAKAE
                     AQIAKMEEQRKANMLAHMETQNKIYNILTPEQKKQFNANFEKRLTERPAAKGKMPATA
                     E"
BASE COUNT         1130 a         1082 c         1085 g         1200 t
ORIGIN      
        1 ctttgccata aagcagcgag tcaattactt caaccggttt gttgggtaac aaacgctgga
       61 tttcgggcaa catataagca atctgcggcc ctaataacac gacatcggca ttctgacctt
      121 tttcaccagc cagtgtttcc ggaaatgctt caataatgac cggaacttca tatttttctg
      181 cctgtgcgcg catttttgat accagtaaag aggtagacat gcccgcagaa caaaacagat
      241 aaatgtgttt cttttccata aaactgccct catcgacgat tatctgtcag ccagacactc
      301 cgcaagcctt aacctgcttc catgctctgg gtaacttgcg aaaccaacat gacttttttt
      361 gtggctgaaa ggagtatacg gtataggcct gagcaatagt atttctttga ccttagaaat
      421 tgtctctcct tgacctgtgg ttatgaccct cttcacattt cgggcaaata attcgcgaag
      481 ataattaaga acacaggcca aaaaaaaacc ggcgcaatgg ccggtttccg ttgttactca
      541 agctttcagg cgaagtgatt acttcgtagc ctgtggatca gtgtcgtatt cagcacaggt
      601 ctgataaccg gagttgatga catgtccggt atcatctaac gcgacaaagt aggtttctgc
      661 tttaccatca cgttgaccca ggatgtaggt ctggcaagta ccacgagcat ggatcatgct
      721 cacttcagac gaaggtttac ccgcaatttg tgcaacctgc gcccggctca tgcctttttt
      781 gacgtctttc accacaggct gtacaaactg gtctttggta cgatcataag ccgtacaacc
      841 cgccagcatg gttaataccg ccgctgcact cagaattcct gccatattct tgttcatatt
      901 ccgtcctctt gtttatcagc gtgttagata agcctggaat acattgggcg ctttttcaag
      961 cccgtgaacg aaacggctcc gctttcagag gattcctgta tgacgtttta accaccattc
     1021 agcccgctgt cgcttgtcgt ttcagtagca acgggttagc tttaaggaag ttttgtcttt
     1081 tctgtctgga ggggttcaat gacattgcaa caacaaataa taaaggcgct gggcgcaaaa
     1141 ccgcagatta atgctgaaga ggaaattcgt cgtagtatcg attttctgaa aagctacctg
     1201 caaacttatc cgttcattaa atcactggtg ctcgggatca gcggcggtca ggactccacg
     1261 cttgccggaa agctgtgcca gatggcgatt aatgagctgc gccaggaaac cgacaacgaa
     1321 tcactgcaat ttattgccgt acgcctgccc tatggtgttc aggccgacga acaagattgc
     1381 caggatgcca ttgcctttat tcaaccggat cgcgtattaa ccgttaatat caagggcgcg
     1441 gtattggcta gcgagcaggc attgcgggaa gcaggcattg aactgagcga ttttgtccgt
     1501 ggcaatgaaa aagcgcgtga gcggatgaaa gcacaatata gcattgcggg tatgaccagc
     1561 ggtgtcgtgg tgggcaccga tcatgcagca gaagccatta ccggattctt cactaaatat
     1621 ggtgacggcg gtacggacat taatccgctg tatcgtctca acaaacgtca gggtaaacag
     1681 ttactggcgg cattaggttg cccggaacac ctttataaga aagcgccaac ggccgatctg
     1741 gaagatgatc gcccttctct gccagatgaa gtggcactcg gcgtgaccta tgacaatatc
     1801 gacgactatc tggaagggaa aaacgtacct caacaggtcg ccagaacaat agagaactgg
     1861 tatctgaaaa ccgaacataa acgccgtccg ccaattaccg ttttcgatga tttctggaaa
     1921 aagtaataat ttgcacatat tggattgtgc gaaaaagagt aatttgttca cgccggatgc
     1981 ggcgtgaacg ccttattcga cctacaaaac tatgcaaatt caatacattg caggagtcga
     2041 ataggcctga caggcgtagc acgtcagacg gtgtaacctt tgtcatcgac ccgcctcttt
     2101 tttaatcgct tcccggctgt tacactggat agataaccag cattcggagt caacagtggt
     2161 acggcgttta acttctccgc ggctcgaatt tgaagctgcg gcaatttatg aatatcccga
     2221 acatttacgt tcattcctta atgacttacc cacccgacca ggggtgtatc tgtttcatgg
     2281 cgaaagtgac accatgccgc tctatatcgg caaaagcgtt aatatccgca gtcgggtgct
     2341 ttctcattta cgcaccccgg atgaagccgc catgctacgg caatcccggc gaattagctg
     2401 gatctgcacc gcaggcgaaa tcggcgctct gctccttgaa gcgcgattaa tcaaagaaca
     2461 acagccgctg tttaataaac ggttgcgccg caatcgccag ctctgtgccc tgcaattaaa
     2521 tgaaaagcgc gtcgatgtgg tgtatgccaa agaggtggat ttttcacgag cccccaacct
     2581 gtttggcctg tttgccaata ggcgcgcagc tttgcaagca ttgcagagca tcgctgatga
     2641 acaaaaactt tgttatggcc tgctgggact ggaaccgtta agccgcgggc gtgcttgttt
     2701 tcgttctgca ttaaaacgtt gcgctggggc gtgttgcggt aaagagagcc atgaggaaca
     2761 tgcgctacgt ttgcgccagt ctctggaacg tttacgagtc gtttgttggc cgtggaaagg
     2821 tgcggtagcg ctgaaagaac agcacccgga aatgactcaa tatcatatta ttcaaaactg
     2881 gctgtggctg ggggcggtta attcgctgaa agaagcgaca acgttaattc gggcacccgc
     2941 cgggtttgat cacgacggtt ataaaattct ttgtaagccg ctgctttccg gtaactacga
     3001 aattactgaa cttgatccgg tgaatgacca gcaagccagt tgatttcact gaacaacagt
     3061 ttaccttgcg gttgcaaaag ccgcagggtg tgcctgccat cgaaccagca cgcgccctgg
     3121 tcggtagtga gcagtttgtc gcccaattgc cacgccccgt ttattacaaa caccaccccg
     3181 ccgcgtgaac caaaggtagt aaacgtacgt tcggcaattc tgactttcgc cttacagaca
     3241 tccaggcgtg tcatgatgtt gaaatccatc gacatctgcc ctgccgtcag tttcgctttt
     3301 accaccaggt ccgctgcaaa ggaaaaaggt tgtaacggtt ttaaggtatg gttaaagcgg
     3361 tctgcgcttt caaggaacat ctcgccgcct tccagcaacg tcactatcct ttccataccg
     3421 ggaaacaaag aaaactcccc attcgccgcg atggatgtaa tgctggcacg ccagtaaaaa
     3481 tcacgttttg ccggtgggaa agtgcaaatt tcgcgcgttt caccggcagc gtttcgccac
     3541 agattcaccg acattttacg catatcaaag tattccatac tcgctcccgg cccgttcttt
     3601 tcattgttat cgttcaggta accgtggatg gttatcggca actcgggtga tttacgttag
     3661 tggcgatcag gctttttttg catctgtttg agaagagaaa agaaaaccgc cgatcctgtc
     3721 caccgcatta ctgcaaggta gtggacaaga ccggcggtct tacgtttttt ggctgaaaga
     3781 ttattcagca gttgcaggca ttttaccttt tgccgctgga cgttctgtca gacgcttctc
     3841 aaaattagca ttaaattgct ttttctgttc cggcgtcagg atgttgtaaa tcttgttctg
     3901 ggtttccatg tgcgccagca tgttagcttt gcgctgttct tccatttttg cgatctgcgc
     3961 ttcagctttt gctttatcga aggtatcgct ggcaatgatg tcatgcattg cgcggcgttc
     4021 ttccagcggc ggacgtttca tctggtcacg ctggcctttc atgatttcgc ggatctgctg
     4081 tttctgcgcg tcggtcaggt tcaggtcttt gaacatcatg tcctcatgcg gaccgaactt
     4141 gcctttatgg tgcatcatcg gcttcgcgtc agccggtgct gcggtagtgg tgtctgcggc
     4201 atgggccagg ttagccgcgc caagagccag ggtagaggca acaaacagtg cagttaattt
     4261 acgcatattc tatatccttc ctttcagtta tttattacgg ctttcttaag tagcgtgccg
     4321 tgttgacgag attaacttta ctgactttag cgtcaattaa tcagagcaac ggtaaaacaa
     4381 tgaaagtgta aaaaacactt ttgcgccaat tatggagaaa aaaagaaaat ttgatggaga
     4441 gtgatgaaag aatattacaa cacgatgatt ttgcagagat tatgaagaac tataccg
//
LOCUS       DADSYM010000215         4442 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2415
            SAMN08039579-rid11723173.denovo.217, whole genome shotgun sequence.
ACCESSION   DADSYM010000215 DADSYM010000000
VERSION     DADSYM010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039579
            Sequence Read Archive: SRR6362533
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4442)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4442)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980162.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 03:39:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,484
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 4,347
            CDSs (with protein)               :: 4,347
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 43 of 85
            Pseudo Genes (incomplete)         :: 37 of 85
            Pseudo Genes (internal stop)      :: 19 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4442
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039579-rid11723173.denovo.217"
                     /strain="LGE2415"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8302 N 95.9628 W"
                     /collection_date="2015-08-24"
     gene            258..1673
                     /gene="gltX"
                     /locus_tag="J1Y83_004132"
     CDS             258..1673
                     /gene="gltX"
                     /locus_tag="J1Y83_004132"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HBA7632223.1"
                     /translation="MKIKTRFAPSPTGYLHVGGARTALYSWLFARNHGGEFVLRIEDT
                     DLERSTPEAIEAIMDGMNWLSLEWDEGPYYQTKRFDRYNAVIDQMLEEGTAYKCYCSK
                     ERLEALREEQMAKGEKPRYDGRCRHSHEHHADDEPCVVRFANPQEGSVVFDDQIRGPI
                     EFSNQELDDLIIRRTDGSPTYNFCVVVDDWDMEITHVIRGEDHINNTPRQINILKALK
                     APVPVYAHVSMINGDDGKKLSKRHGAVSVMQYRDDGYLPEALLNYLVRLGWSHGDQEI
                     FTREEMIKYFTLNAVSKSASAFNTDKLLWLNHHYINALPPEYVATHLQWHIEQENIDT
                     RNGPQLADLVKLLGERCKTLKEMAQSCRYFYEDFAEFDADAAKKHLRPVARQPLEVVR
                     DKLTAITDWTAENVHHAIQATADELEVGMGKVGMPLRVAVTGAGQSPALDVTVHAIGK
                     TRSIERINKALAFIAERENQQ"
     gene            complement(1674..2215)
                     /gene="tnpA"
                     /locus_tag="J1Y83_004133"
                     /pseudo
     CDS             complement(1674..2215)
                     /gene="tnpA"
                     /locus_tag="J1Y83_004133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311304.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
     gene            2223..3431
                     /gene="tnpB"
                     /locus_tag="J1Y83_004134"
                     /pseudo
     CDS             2223..3431
                     /gene="tnpB"
                     /locus_tag="J1Y83_004134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001458895.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family element transposase accessory
                     protein TnpB"
     gene            complement(3471..3863)
                     /locus_tag="J1Y83_004135"
     CDS             complement(3471..3863)
                     /locus_tag="J1Y83_004135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416898.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding transcriptional regulator"
                     /protein_id="HBA7632224.1"
                     /translation="MKRLRNKMTTEELAECLGVAKQTVNRWIREKGWKTEKFPGVKGG
                     RARLILVETQVCEFIQNTPAFHNTPMLLEAEEPLAEYAPGIRTPAYRQIISAIDNMTH
                     SEQEKVAQFLSREGIRNFLARLDIDESA"
     gene            complement(3865..4209)
                     /locus_tag="J1Y83_004136"
     CDS             complement(3865..4209)
                     /locus_tag="J1Y83_004136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708260.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding transcriptional regulator"
                     /protein_id="HBA7632225.1"
                     /translation="MTPDELARLTGYSRQTINKWVRKEGWTTSPKPGVQGGKARLVHV
                     NEQVREYIRNAERPEGQGEAPALSGDAPLEVLLVTLAKEMTPVEQKQFTSLLLREGII
                     GLLQRLGIRDSK"
BASE COUNT         1131 a         1077 c         1139 g         1095 t
ORIGIN      
        1 cgctgtgtgg agtcgcatta tagggagagt tcaaaatgag tcaacgcatt ttctaaagaa
       61 attgttcgtt cgtcgtaaat ttaagcaaga tgatcgcaaa acagaccgtg ttgcgcaatt
      121 tgtcaacgaa aacaataatg cgtaaggttg caacccgaac tacattgagg aatcaggcgg
      181 gagtgataga atatcgccca ctcaattttt ccaggatttg ccggttgtcg gcatctttct
      241 aaacgtaagg ccatttcatg aaaatcaaaa ctcgcttcgc gccaagccca acaggctatc
      301 tgcacgttgg cggcgcgcgt actgctcttt actcctggct ttttgcacgt aaccacggcg
      361 gtgagttcgt gctgcgtatt gaagacaccg atcttgagcg ttccacgccg gaagctatcg
      421 aagccattat ggatggcatg aactggctga gcctggagtg ggatgaaggt ccgtactacc
      481 agaccaaacg ttttgatcgc tacaacgcgg tgatcgatca gatgctggaa gagggcacag
      541 cttataaatg ctattgctct aaagagcgcc tggaagcgct gcgcgaagag caaatggcga
      601 aaggtgagaa gccgcgttat gacggtcgct gccgccacag tcatgagcat cacgctgatg
      661 atgagccgtg tgtcgtacgt tttgctaacc cgcaggaagg ttctgttgtt tttgacgatc
      721 agatccgtgg tccgatcgag ttcagcaacc aggagctgga tgatctgatt atccgccgta
      781 ccgatggttc cccaacctat aacttctgtg tggttgtcga tgactgggat atggaaatca
      841 cccacgttat ccgtggcgaa gaccatatca acaacacacc gcgccagatt aacattctta
      901 aggctctgaa agcgccggta ccggtttacg cgcacgtttc tatgatcaac ggcgatgacg
      961 gtaaaaaact gtccaaacgt cacggggcgg tcagcgtaat gcagtatcgt gatgatggtt
     1021 atctgccaga agcactgctg aactatctgg tgcgtctggg ctggtcccac ggcgatcagg
     1081 aaatcttcac tcgtgaagag atgattaaat acttcactct gaatgccgtc agcaaatctg
     1141 ccagtgcgtt caacaccgac aagctgctgt ggctgaacca tcactacatt aacgcgttgc
     1201 cgccggagta tgttgctact cacttacagt ggcacattga gcaggaaaat atcgataccc
     1261 gtaacggccc gcagctggct gatctggtga aactgttggg cgaacgctgc aagacgctga
     1321 aagagatggc acagagctgc cgttatttct acgaagattt tgctgagttc gatgccgacg
     1381 ccgcgaaaaa acatctgcgt ccggtagcgc gccagccgct ggaagtggtt cgtgacaaac
     1441 tgactgcgat tactgactgg accgctgaaa acgttcatca cgctattcag gcgacggcgg
     1501 atgagctgga agtgggtatg ggtaaagttg gtatgccgct gcgtgtcgcc gtaaccggtg
     1561 cggggcagtc tccggcgctg gatgttaccg ttcacgcaat cggtaagacc cgcagcatcg
     1621 agcgtatcaa caaagcgctg gcttttattg ctgaacgcga aaatcagcag taattagcgc
     1681 ataaaagata aacggcagga gagatacccc tgccgttttt tatttatccg gtgcgccgta
     1741 aaaccccgtc cttcagggcg gggatataag gcgcggtttt ccacctgacc aggtgtttgc
     1801 tgttgttcaa tgtattggcg gatgacggat attggcgcac ctccgcagct actggcaaaa
     1861 tagccaggac tccacaaaat gcctttgtag taatacctga ctgcaatatc tggtcgatct
     1921 cgtcgcagtc acctacccga tacgcctttg aggctgttta ccagactgga tatcgccagt
     1981 ttgggaggat agttaattaa caaatggaag tgatctggtt cgccatccat ttcaacccgt
     2041 tcagcttcaa aatcagcaca tacatttgaa tagtaagtgc gtagtttttc tgtcgcgtca
     2101 tggtcaaaaa tctggcgtcg gtatttggga caaagaccag gtgaacatgc atcaggaaaa
     2161 cacaatgcct tccacgccgg atatcggttt cttttttcac agaccaaagt atcatttggc
     2221 ctgtgaaacg actacaggca tttaaattcc agttaagacc cggtggtcaa caggagcgtg
     2281 aaatgaggcg cttcgcaggc gcatgtcgtt tcgttttcaa tcgtgctctg gcgcttcaga
     2341 atgagaatca tgaggccggt aataaataca tcccttacgg gaaaatggct tcctggctgg
     2401 ttgagtggaa aaatgccact gaaacgcaat ggcttaaaaa tgccccgtca cagccattgc
     2461 aacagtcact gaaagacctt gagcgggctt acaaaaactt cttccagaag cgggctgctt
     2521 ttccccgatt caaaaagcgg ggacagaatg attcattccg ctacccgcag ggcgttaagc
     2581 tcgatcagga aaacagccgt atttttctgc cgaaactggg ctggatgcgc taccggaata
     2641 gccgtcaggt cacgggtgtt gtgaaaaatg tcacggtcag ccagtcctgc ggtaagtggc
     2701 acatcagtat tcagacagaa agtgaagtat caactccggt tcacccttca gcatcaatgg
     2761 tcgggctgga tgctggcgtg gctaaactcg ccacgctgtc agatggcaca gtctttgagc
     2821 ctgtaaacag ttttcagaaa aaacagaaga cgctggcgag acttcagcga cagttaagcc
     2881 gcaaggtcaa attcagcaat aactggcaga agcagaaacg caaaatacag cgactgcatt
     2941 cccgtatcgc aaatatccgc agggactacc ttcacaaagt cacaacgacc gtcagcaaaa
     3001 accacgcaat gatagtcatt gaggatttga aggtcagcaa catgtcaaag tcagcagcgg
     3061 gtacggtcag ccagccgggg cgcaatgtcc gggcaaaatc aggtttaaac cgttcgatac
     3121 tggatcaggg ctggtatgaa atgcgccgcc agcttgagta caagtagctc tggcgtggcg
     3181 gtcaggtgct tgctgttccg ccagcgtaca caagccagcg ttgcgcgtgc tgtggtcata
     3241 cagcgaaaga aaatcgcctg tcacaaagta aattcagatg ccaggtatgt ggatatacag
     3301 cgaacgccga tgtaaacggc gctcgtaaca ttttagcggc ggggcacgcc gttcttgcct
     3361 gtggagagat ggtgcagtca ggccgcccgt tgaagcagga acccaccgaa atgattcagg
     3421 cgacagcctg aacgtagcag ggatcctcgt cattcagggc gtggaggatg tcaagctgat
     3481 tcatctatgt cgagacgagc aagaaaatta cgaattcctt cgcgtgacaa aaattgcgca
     3541 actttttctt gctcactgtg agtcatgttg tctattgcgc tgatgatttg ccgataagca
     3601 ggagtgcgaa tacccggtgc gtattcggca aggggttctt cggcttccag aagcatcggt
     3661 gtgttatgga aggctggcgt gttctgaata aactcgcaaa cttgcgtttc gaccagaata
     3721 agtctggcac gaccgccttt cacgccggga aatttttccg ttttccagcc tttttctctg
     3781 atccaacggt taacagtttg tttagccaca ccgaggcatt cagccagttc ttcggtggtc
     3841 attttattgc gtaatctttt catattattt gctatcgcga atccctaagc gttgtaacaa
     3901 tccgataatc ccttcccgca gaagcaagga tgtaaactgt ttttgttcaa ctggcgtcat
     3961 ctctttcgcc agtgtcacca gcaacacttc aagcggcgca tcaccagaaa gggctggcgc
     4021 ttctccctga ccttctggac gttcagcatt gcgaatatat tcacgaacct gttcattgac
     4081 gtgaaccagt cgagctttgc caccctggac gcctggtttt ggtgacgttg tccagccttc
     4141 cttgcgtacc catttattaa tggtctggcg gctatagccg gtcagtctgg caagttcatc
     4201 tggcgtcatc ctctccttga acataacaat tccctgaatg tatgtgggtc tattagtagt
     4261 tcattttata acaccacttt acaggaaacc gtgacatgaa taactactga atgcgagcgg
     4321 ttttccaaaa ttgttcattt gcctgatttt tcagcgattg aaactacacg tggcaatttg
     4381 ccgttgacac attcgggcgg aattcatatg atgccgcccg tcaactcgac aagctttacg
     4441 tg
//
LOCUS       DADSYM010000216         4442 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2415
            SAMN08039579-rid11723173.denovo.218, whole genome shotgun sequence.
ACCESSION   DADSYM010000216 DADSYM010000000
VERSION     DADSYM010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039579
            Sequence Read Archive: SRR6362533
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4442)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4442)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980162.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 03:39:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,484
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 4,347
            CDSs (with protein)               :: 4,347
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 43 of 85
            Pseudo Genes (incomplete)         :: 37 of 85
            Pseudo Genes (internal stop)      :: 19 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4442
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039579-rid11723173.denovo.218"
                     /strain="LGE2415"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8302 N 95.9628 W"
                     /collection_date="2015-08-24"
     gene            <1..425
                     /locus_tag="J1Y83_004137"
     CDS             <1..425
                     /locus_tag="J1Y83_004137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001764492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1795 domain-containing protein"
                     /protein_id="HBA7632226.1"
                     /translation="VTFPGNIIQDSTINMLRLSDPDAALIISRGQMQEGDDLTSQIEQ
                     QMKKLEKQVKDLHYTPVQVTRVGINDGEEGLEIQSQFLRGNEQVYQCQVAFVLPGERV
                     MMAFTYARTTPLTLADMTRWAEIKKNLRFRMRQEAGTN"
     gene            444..4148
                     /locus_tag="J1Y83_004138"
                     /pseudo
     CDS             444..4148
                     /locus_tag="J1Y83_004138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000390554.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat protein"
BASE COUNT         1002 a         1155 c         1501 g          784 t
ORIGIN      
        1 ttgtaacttt tcccgggaat attattcagg acagtacgat taatatgctg cgcctcagcg
       61 atcccgacgc tgcactcatt atcagccggg gccagatgca ggaaggagac gacttaacct
      121 cccagattga acagcaaatg aaaaaactgg agaaacaggt aaaggatctt cactacacgc
      181 ccgttcaggt aacacgggtg gggattaatg atggtgaaga agggctggaa atacagagcc
      241 agtttctccg gggtaacgag caggtgtacc agtgtcaggt ggcatttgta ttgccgggtg
      301 agcgggtcat gatggccttc acctatgccc gaaccacacc gctgaccctg gcagatatga
      361 cgcgctgggc tgaaattaaa aagaatctgc gcttcaggat gcggcaggaa gccgggacga
      421 attaatcaca caggaaacgg gacatggaag aggcattctg ggcggcaaga cagggtgatg
      481 ccctgctgca tacgtcgttt atggcagatg tactgggggc ggtgctggag gtggccgcaa
      541 atgtcgttat tgatgccctc attgtcggca caacctcgtt actggcggcg tcagtgggga
      601 tcacgatggg gtgcacagcc gtcgtgctga ccggttttgt ggcgggtgtg gcgatggtgt
      661 acaccggcgt gacagaaaag gtcagcgaag cctgttcagc cctcgcagat atgcttttcc
      721 cgcctcagac tgaaggttac atcctcaccg gctctggtga cacatggatt aacagcaaac
      781 ctgccgcgcg ggcagcggcg actgcggcat cgcggcagga cattgaggcg caggaagcgc
      841 aggcgaaggc ggaacaggaa gaagcacagc gtcaggagga ggccagaacg ttccgggatg
      901 tggccgggga atatctggaa atggcggggg ccatagccct ggcagttaac ccggtgacag
      961 ggcctgtgct gatgtcccgg gcgctgcacg gccagctcag tacggaagaa gggcgttctg
     1021 aaatggtgga tggcgtgaca cattttgtgt cggaactatg gcagcccacg gtggccggtg
     1081 cggctcctgg ctccaccctg actcctgacg ataaaatcga ctgccataaa catccctcct
     1141 ctctgacgca gttactcgca caaaaaaaag ccgcctttct ggatgaccct ccgggaacgc
     1201 tccttaatct gttgagtccg gacggtatgc tggaaaccgc ctttcaggga gtgaatgcgg
     1261 ttattggcag catcagtaat ctgttcaaag gcgatgacga accgcccgcg gcggaatata
     1321 tcgcggaagg cgcgcgggat gtgcgtatca acagccagcc ggcagcacgc agcggggcac
     1381 gctgtacctg cgaggccagg gtggtgaatg cgccggggaa cggggcgttt gtgtcaccgg
     1441 atgtgcgcat tggcggtccg ttactggtgg tcagggatat cagaagcggc aggtcgcaga
     1501 taaccctggt gtcaaccgtg gcgctgatgt ttcttcgccc gggtaaaatg ctttctgaaa
     1561 tcgcctgttt tgctgccggt atcgggatga gcattctgac gcagaaggcc gccacagccc
     1621 ttccgcatcc ggtcagtgcc gccaccgggg ccaaatacct ggccggtgat gatgacttcg
     1681 atttcagtct gccgggccac tttccgctgg actggcagcg cgtgtacagc agtcgtgacg
     1741 aacgcacgga aggcatgttt ggtcagggct ggagtgtaat gtatgaagtc agcctcgtgc
     1801 gcacaccggg tagcgcagac gaaaactgca tgacctttgt gtcgggtatg ggacgacgcc
     1861 tggagatgga agccgtactg ccggggggcg ggttttacag ccccggcgaa ggactggcgg
     1921 tacggcgggg tgagcagggg cactggctta tcagcagcga tgatggacag ttttttctgt
     1981 ttgaggccga cccgcaccac ccgcagcgcc agcggctgaa aatgctgggc gaccgcaaca
     2041 gcaacagtct gaatctgtac tacgacgacc ggggacgcat cactgaaatc agcggcgagc
     2101 agcagcgtcc gtgcatccgt ctgtattacg aactggcggc gcatccccgg cgggtgacac
     2161 agatttatca gcatttccct gatacgacgc ctttactgct gcgtcgttac cgctatgacg
     2221 gggccggcga cctgaacggc gtgtacgaca gcacggggca cctgctgcgt gagtttgctt
     2281 acgacgaaaa ccactgcatg acgctgcacc gccagccggg cggcgaaggg tattattacc
     2341 agtggggctg gtatgaaggg ccggatgacg cggcatggcg ggtgacgggg catcacacgg
     2401 acagcggtga acagtaccgg ctggcgtgga gcctggcggc acgcaggctg tgcgtgacgg
     2461 acgggctggg gcgcacgcgt tatcatcagt gggacgcgca gaatcaggtg acggcgtacc
     2521 aggacgaagc cggacaggtg acgacgttcc gctggagcga cgaagaacgg ctgctgctgg
     2581 gaatgacgga tgcgcagggc ggcaaatggc gctatgtgta tgaccggcag gggcatatca
     2641 cggagacgca cgacccgctg ggacgggtgg agcaggcgca gtggcacccg gtgtggcatc
     2701 agccggaaac ggaggtggac gcggcgggaa acagctggcg ctgtgtgtac gatgaacgcg
     2761 gcaaccttct tgccgtgacc gacccgctgc agcagaacac ccggtatcag tatgaccgcc
     2821 acggtcaggt ggtgcagata acggatgcgc gcggggggaa taaatacctc cagtggaatg
     2881 aagacgggca gctcatgcgg cacacggact gctccggctc gcagacggcg tggttctacg
     2941 acgaacgcac gcggctcatc agaatgacgg atgcggagag ccacagcacg cgctacgggt
     3001 atgacgacag cggccacctg acggaggtga tactggcgga cggccgcacg gtgaattacc
     3061 agtcggacgc ggcgggcagg ctggtgaagt acaccagccc gatggggagg ataacgcgct
     3121 ggcagcgtga cgggcagggc cgggtgcgca gccggactga cgcgacggga cgcaggacgg
     3181 cgttcgggta cgacgcttac gggcgtctgg tcacgctgac aaacgagaac ggagaaagct
     3241 accggttccg ccatgacgtg ctggaccgtc tggcggagca gataaacccg gacggctgcc
     3301 gccaggcgta ccgctacaac gcgctgaacg cggtcacgga agtggtgttc accggggacc
     3361 ggggcggtga aatccgtcac cggctggcgc gtgacgcggc gggccggctc acggcaaagg
     3421 agacagccgg tagccgtacg gaatatgtcc atgatgcggc agaccagcta ctggaaatcc
     3481 gccgccggag gccggatgcc ggggaaacgg acgccccgga gattatccgt ttcagttacg
     3541 acagactggg gcggatgctg agcgaggaga cggcgcaggg catcctgacc catcagtacg
     3601 atgaactcag taaccggact gccaccacct ttccggacgg cagaacgcag cgccatctgt
     3661 actacggcag cggccatctc cagcagataa atctggaccg ggaggtcatc agcgagttca
     3721 cgcgcgacca cctgcaccgt gaagtgctca gaagtcaggg acgactgagc acgcggcagc
     3781 tttacgaccc cggcgggcga ctgaagcgca gggagacata cagcgggatg cgcggggtgg
     3841 tcccggagac gttcacggac cggcagtaca gctacagcgg cgaggatgaa ctgctgaaga
     3901 cgcggcacag ccggcggggg gaaacggact acttctatga caccacgggg cgcatcacgg
     3961 cgtgtcgctc ggaggatgag gggtatctgg caagctggga gtacgacgcg gcgggcaacc
     4021 tgctggacag gcgcgcgggg ggaagggcca cggcagaaaa cagcgtggtg ccgttcaacc
     4081 ggctgatgtc ttaccgtggt gtgcactacc gttatgacga atttggccgt gcggtggaaa
     4141 aggaggggcc tcaaggaacc gttgtcacag ttaaatcaaa acaggaagcc catgatttat
     4201 tgatggaggc ctttcctgat gctcaaaaag taagagggat cggttcgcag gatgccgccg
     4261 gtattagaaa aaaacataag atggaacaat ttaagaaaag agatggtact gtccgttata
     4321 ggaaagatta ccctattgat tctaatactg gtcgtgtata tggtcatgat gaccctaaag
     4381 gcacagggca tggttctttg cctcatataa atattaaacg ttctgatggg acaatggtga
     4441 gg
//
LOCUS       DADSYM010000217         4362 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2415
            SAMN08039579-rid11723173.denovo.219, whole genome shotgun sequence.
ACCESSION   DADSYM010000217 DADSYM010000000
VERSION     DADSYM010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039579
            Sequence Read Archive: SRR6362533
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4362)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4362)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980162.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 03:39:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,484
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 4,347
            CDSs (with protein)               :: 4,347
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 43 of 85
            Pseudo Genes (incomplete)         :: 37 of 85
            Pseudo Genes (internal stop)      :: 19 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4362
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039579-rid11723173.denovo.219"
                     /strain="LGE2415"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8302 N 95.9628 W"
                     /collection_date="2015-08-24"
     gene            525..1490
                     /gene="lipA"
                     /locus_tag="J1Y83_004139"
     CDS             525..1490
                     /gene="lipA"
                     /locus_tag="J1Y83_004139"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HBA7632227.1"
                     /translation="MSKPIVMERGVKYRDADKMALIPVKNVATEREALLRKPEWMKIK
                     LPADSTRIQGIKAAMRKNGLHSVCEEASCPNLAECFNHGTATFMILGAICTRRCPFCD
                     VAHGRPVAPDANEPVKLAQTIADMALRYVVITSVDRDDLRDGGAQHFADCITAIREKS
                     PQIKIETLVPDFRGRMDRALDILTATPPDVFNHNLENVPRIYRQVRPGADYNWSLKLL
                     ERFKEAHPEIPTKSGLMVGLGETNEEIIEVMRDLRRHGVTMLTLGQYLQPSRHHLPVQ
                     RYVSPDEFDEMKAEALAMGFTHAACGPFVRSSYHADLQAKGMEVK"
     gene            complement(1590..1793)
                     /gene="tatE"
                     /locus_tag="J1Y83_004140"
     CDS             complement(1590..1793)
                     /gene="tatE"
                     /locus_tag="J1Y83_004140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatE"
                     /protein_id="HBA7632228.1"
                     /translation="MGEISITKLLVVAALVVLLFGTKKLRTLGGDLGAAIKGFKKAMN
                     DDDAAAKKGADVDLQAEKLSHKE"
     gene            complement(1921..2709)
                     /locus_tag="J1Y83_004141"
     CDS             complement(1921..2709)
                     /locus_tag="J1Y83_004141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deaminated glutathione amidase"
                     /protein_id="HBA7632229.1"
                     /translation="MLVAAGQFAVTSVWEKNAEICGSLMAQAAENDVSLFVLPEALLA
                     RDDHDADLSVKSAQLLEGEFLGRLRRESKRNMMTTILTIHVPSTPGRAWNMLVALQAG
                     NIVARYAKLHLYDAFAIQESRHVDAGNEIAPLLEVEGMKVGLMTCYDLRFPELALAQA
                     LQGAEILVLPAAWVRGPLKEHHWSTLLAARALDTTCYMVAAGECGNKNIGQSRIIDPF
                     GVTIAAASEMPALIMAEVTPERVRQVRAQLPVLNNRRFAPPQLL"
     gene            2805..3188
                     /gene="crcB"
                     /locus_tag="J1Y83_004142"
     CDS             2805..3188
                     /gene="crcB"
                     /locus_tag="J1Y83_004142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HBA7632230.1"
                     /translation="MLQLLLAVFIGGGTGSVARWLLSMRFNPLHQAIPLGTLAANLIG
                     AFIIGMGFAWFSRMTNIDPVWKVLITTGFCGGLTTFSTFSAEVVFLLQEGRFGWALLN
                     VFVNLLGSFAMTALAFWLFSASTTH"
     gene            complement(3242..3451)
                     /gene="cspE"
                     /locus_tag="J1Y83_004143"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
     CDS             complement(3242..3451)
                     /gene="cspE"
                     /locus_tag="J1Y83_004143"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002436076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator/RNA stability
                     regulator CspE"
                     /protein_id="HBA7632231.1"
                     /translation="MSKIKGNVKWFNESKGFGFITPEDGSKDVFVHFSAIQTNGFKTL
                     AEGQRVEFEITNGAKGPSAANVIAL"
     gene            complement(3626..4186)
                     /locus_tag="J1Y83_004144"
     CDS             complement(3626..4186)
                     /locus_tag="J1Y83_004144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid:lipid A palmitoyltransferase"
                     /protein_id="HBA7632232.1"
                     /translation="MNVSKYVAIFFFVFIQLISVGKVFANADEWMTTFRENIAQTWQQ
                     PQHYDLYIPAITWHARFAYDKEKTDRYNERPWGGGFGQSRWDEKGNWHGLYAMAFKDS
                     WNKWEPIAGYGWESTWRPLADENFHLGLGFTAGVTARDNWNYIPLPVLLPLASVGYGP
                     ATFQMTYIPGTYNNGNVYFAWMRFQF"
BASE COUNT         1167 a         1114 c          963 g         1118 t
ORIGIN      
        1 gaaaatcgtc agaaggtaaa ccacaaatat ttacaacctt acgaaatatt gatcttaccc
       61 ttctgactat tttgaagttg catatgtcca taaaggaatc gttaatgcag cgaaagtgct
      121 taatctgccc cccctcggtc ggagccctgg tccttcctgt catctatcgg gcgattaaaa
      181 ctcactatcc acagctttta ctgcgcaacc cgcccatcgg cggcgcggaa aaccaactca
      241 gtcagtttca aaccgatctc atcaccgata acatgttttg cacaggacca tagatgttat
      301 ccgtaatgca ttttgaaaaa gtattgttac agccagatca cagcgcagaa cgataaaatg
      361 gcacaatcca acaacaattt tacattttcg cgaccgcttt ggctgctttc aggtccgttt
      421 caatgatata ctgccagtcg ttaattcaaa aatagttgat aattacaaca atctattgaa
      481 ttgaaacgct ttccttcata attcgcaact ggaacacgca cgctatgagt aaacccattg
      541 tgatggaacg cggtgttaaa taccgcgatg ccgataagat ggcccttatc ccggttaaaa
      601 acgtggcaac agagcgcgaa gccctgctgc gcaagccgga atggatgaaa atcaagcttc
      661 cagcggactc tacacgtatc cagggcatca aagccgcaat gcgcaaaaat ggcctgcatt
      721 ctgtctgcga ggaagcctcc tgccctaacc tggcggaatg ctttaaccac ggcacagcaa
      781 cgtttatgat cctcggcgct atttgtaccc gccgttgccc gttctgtgac gttgcccacg
      841 gtcgcccggt agctcctgat gccaatgaac cagtgaaact ggcgcagacc attgccgata
      901 tggcgctgcg ttatgtggtt atcacctccg ttgaccgtga tgacctgcgc gatggcggtg
      961 cccagcactt tgcggattgc attactgcca ttcgggaaaa aagcccgcaa atcaaaattg
     1021 aaactctggt gccggatttc cgcggtcgta tggatcgtgc tctggatatt ctgactgcaa
     1081 cgccaccaga tgtgttcaac cataacctgg aaaacgtacc gcgtatttac cgtcaggtac
     1141 ggcctggtgc agattacaac tggtcgctta agctgctgga acgctttaaa gaagcgcatc
     1201 cggaaatccc gaccaagtct ggtctgatgg tgggactggg tgaaaccaat gaagaaatta
     1261 ttgaggtaat gcgcgacctg cgccgtcatg gtgtgacgat gctaacgctg gggcaatatt
     1321 tgcagccaag ccgccatcac ctgccggttc aacgttacgt tagcccggat gagttcgacg
     1381 aaatgaaagc cgaagcgctg gcgatgggct ttacccatgc tgcatgcggt ccgtttgtcc
     1441 gctcttctta ccacgccgat ttgcaggcga aagggatgga agttaagtaa tcctgtaata
     1501 attacttaca tctgtctgat aaaaaacccg ctttttgaag cgggtttttg tatcgaacaa
     1561 gatattgagg gagcgtcctg ctcgccacgt cactctttat gagagagctt ttcagcctga
     1621 agatcaacgt ctgcgccttt tttcgccgca gcatcgtcat cattcatcgc cttcttgaac
     1681 cctttaatgg ccgctccaag gtctccgccc agcgtacgta acttcttagt cccaaacagc
     1741 agaacgacca gcgccgcaac taccagcagt ttggtaatac taatctcacc catagatacc
     1801 ttcttgacat ataaccggca acagaacgcg ccttaaaaac cagagaatat taacggtcat
     1861 tcgacgcgcg cacacaatag caatctgtaa caaggtgaat caagcaccga gtaaaaaaca
     1921 tcataataat tgcggcggcg caaagcgacg gttgtttaag acgggcagtt gcgcgcgtac
     1981 ctgacgcaca cgttcgggcg tcacttccgc cataatgagt gcaggcattt ctgacgctgc
     2041 cgcaatggtg acgccaaagg gatctataat ccggctttgc ccgatatttt tgttcccgca
     2101 ctcccccgcc gccaccatat aacaggtggt atccagcgca cgagcggcaa gcaacgttga
     2161 ccagtgatgc tctttgagcg gcccacgaac ccaggcggca ggaagtacca ggatttcagc
     2221 tccctgtaat gcctgtgcca gcgccagctc tggaaagcgt aagtcataac aggtcatcag
     2281 accgaccttc atcccttcca cctccagtaa cggagctatt tcattaccgg catcaacatg
     2341 gcgtgattcc tgaatggcaa atgcatcata gagatgcagt ttggcataac gggcgacgat
     2401 gtttcctgcc tgtagcgcca ccagcatatt ccatgcgcgc cccggcgttg aaggaacatg
     2461 aatcgtcaga attgtcgtca tcatgttacg tttactttct cgccgtaaac gtccgaggaa
     2521 ttcgccttcc agcagctgtg ctgatttaac cgatagatct gcatcatgat catcacgcgc
     2581 caacaatgct tccggcagga caaacagcga aacgtcgttt tccgccgcct gcgccatcaa
     2641 cgagccacaa atctcagcgt tcttttccca cacagatgta acagcaaact gtcctgcggc
     2701 aaccagcatt gccacctcct cctgtcagag tcgtaaaatt cgccaacggc gattattcgg
     2761 cgttacactt atcactcata caaatcaaat agcaggattt tgcagtgtta caacttcttt
     2821 tagcagtttt tataggcggt ggtacgggaa gcgtggcgag atggctgtta agtatgcgat
     2881 ttaacccgct gcatcaggcg attccgttgg ggacgctggc agcaaacctg attggggcat
     2941 tcatcatagg aatgggattc gcctggttca gcaggatgac gaacattgat ccagtgtgga
     3001 aagtattaat caccaccgga ttttgtggcg gtctaacaac cttctcaaca ttttcggcag
     3061 aagtggtgtt tttgttacaa gagggccgct ttggctgggc gttactgaac gttttcgtca
     3121 atcttttggg gtcttttgcc atgaccgcac tggctttctg gctgttttcg gcctcaacca
     3181 cacactaaag accaaaaaaa acccgctgat taagcgggtt ttgaattctt gctgacgtat
     3241 cttacagagc gattacgttt gcagcagaag ggcctttggc accgttagtg atttcgaact
     3301 ctacgcgctg accttcagca agagttttaa aaccattagt ctggattgca gagaagtgta
     3361 cgaacacgtc tttgctgccg tcttccggag taatgaaacc gaatcctttg gactcattaa
     3421 accacttaac gttaccttta atcttagaca tcaaaattac ctttacatga aaaatagaca
     3481 caaatgctgt gtcggttacc agtacaccaa ttgtggtacg ctttgtccag tgcaacttta
     3541 ctaaaaagtg ataaaagtcg cgttattttt gatgttaatt attttagtgg ttgattttaa
     3601 agaatttact aaaacttcat ttgtctcaaa actgaaagcg catccaggca aagtacacat
     3661 tgccattgtt gtaagtaccc ggaatgtagg tcatctgaaa cgtcgcgggg ccatacccca
     3721 cagaggccaa tggcagtaga accggaagag ggatgtaatt ccagttatcg cgcgccgtta
     3781 cgccagcggt aaatcccagg cctaaatgaa aattttcatc cgccagcggt cgccaggtac
     3841 tttcccatcc gtatccggca atcggttccc atttgttcca cgagtcctta aatgccattg
     3901 catacaggcc atgccagttt cctttttcat cccaacgcga ctgaccaaaa ccgccacccc
     3961 acggtcgctc gttatagcga tcggtttttt ctttgtcgta agcaaaacgt gcatgccagg
     4021 tgatggcagg aatatataaa tcataatgtt gtggctgttg ccaggtttgt gcaatatttt
     4081 ctctaaacgt tgtcatccac tcatcagcgt tagcaaaaac tttaccaaca ctgattaact
     4141 gaataaaaac aaagaaaaat atagcgacat atttactcac gttcatttgt gaccattaaa
     4201 catttatcaa aaatctacta ctaacatagc aaagctacaa ttaacataac ctcaatagcc
     4261 ccaacatgaa gaataatatg aatagctgcc ctatttattt ccgctagttc aagataaata
     4321 tttccgcgca tcgattaaag acgacgcatc gttttctgcg at
//
LOCUS       DADSYM010000218         4218 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2415
            SAMN08039579-rid11723173.denovo.220, whole genome shotgun sequence.
ACCESSION   DADSYM010000218 DADSYM010000000
VERSION     DADSYM010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039579
            Sequence Read Archive: SRR6362533
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4218)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4218)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980162.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 03:39:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,484
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 4,347
            CDSs (with protein)               :: 4,347
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 43 of 85
            Pseudo Genes (incomplete)         :: 37 of 85
            Pseudo Genes (internal stop)      :: 19 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4218
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039579-rid11723173.denovo.220"
                     /strain="LGE2415"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8302 N 95.9628 W"
                     /collection_date="2015-08-24"
     gene            complement(<1..233)
                     /locus_tag="J1Y83_004145"
     CDS             complement(<1..233)
                     /locus_tag="J1Y83_004145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HBA7632233.1"
                     /translation="MSKEKFERTKPHVNVGTIGHVDHGKTTLTAAITTVLAKTYGGAA
                     RAFDQIDNAPEEKARGITINTSHVEYDTPTRHYA"
     gene            complement(348..423)
                     /locus_tag="J1Y83_004146"
     tRNA            complement(348..423)
                     /locus_tag="J1Y83_004146"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(388..390),aa:Thr,seq:ggt)
     gene            complement(430..504)
                     /locus_tag="J1Y83_004147"
     tRNA            complement(430..504)
                     /locus_tag="J1Y83_004147"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(469..471),aa:Gly,seq:tcc)
     gene            complement(621..705)
                     /locus_tag="J1Y83_004148"
     tRNA            complement(621..705)
                     /locus_tag="J1Y83_004148"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(669..671),aa:Tyr,seq:gta)
     gene            complement(714..789)
                     /locus_tag="J1Y83_004149"
     tRNA            complement(714..789)
                     /locus_tag="J1Y83_004149"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(754..756),aa:Thr,seq:tgt)
     gene            1151..2101
                     /gene="coaA"
                     /locus_tag="J1Y83_004150"
                     /gene_synonym="panK"
     CDS             1151..2101
                     /gene="coaA"
                     /locus_tag="J1Y83_004150"
                     /gene_synonym="panK"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HBA7632234.1"
                     /translation="MSIKEQTLMTPYLQFDRNQWAALRDSVPMTLSEDEIARLKGINE
                     DLSLEEVAEIYLPLSRLLNFYISSNLRRQAVLEQFLGTNGQRIPYIISIAGSVAVGKS
                     TTARVLQALLSRWPEHRRVELITTDGFLHPNQVLKERGLMKKKGFPESYDMHRLVKFV
                     SDLKSGVPNVTAPVYSHLIYDVIPDGDKTVVQPDILILEGLNVLQSGMDYPHDPHHVF
                     VSDFVDFSIYVDAPEDLLQTWYINRFLKFREGAFTDPDSYFHNYAKLTKEEAIKTAMT
                     LWKEINWLNLKQNILPTRERASLILTKSANHAVEEVRLRK"
     gene            complement(2130..3095)
                     /gene="birA"
                     /locus_tag="J1Y83_004151"
     CDS             complement(2130..3095)
                     /gene="birA"
                     /locus_tag="J1Y83_004151"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional biotin--[acetyl-CoA-carboxylase]
                     ligase/biotin operon repressor BirA"
                     /protein_id="HBA7632235.1"
                     /translation="MKDNTVPLKLIALLANGEFHSGEQLGETLGMSRAAINKHIQTLR
                     DWGVDVFTVPGKGYSLPEPIQLLNAEKILSQLDDGSVAVLPVIDSTNQYLLDRIGELK
                     SGDACVAEYQHAGRGRRGRKWFSPFGANLYLSMFWRLEQGPAAAIGLSLVIGIVMAEV
                     LRKLGADKVRVKWPNDLYLQDRKLAGILVELTGKTGDAAQIVIGAGINMAMRRVEESV
                     VNQGWITLQEAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPV
                     KLIIGDKEIFGISRGIDKQGALLLEQDGIIKPWMGGEISLRSAEK"
     gene            complement(3092..4120)
                     /gene="murB"
                     /locus_tag="J1Y83_004152"
     CDS             complement(3092..4120)
                     /gene="murB"
                     /locus_tag="J1Y83_004152"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HBA7632236.1"
                     /translation="MNHSLKPWNTFGIDHNAQHIVCAEDEQQLLNAWQHATAEGQPVL
                     ILGEGSNVLFLEDYRGTVIINRIKGIEIHDEPDAWYLHVGAGENWHRLVKYTLQEGMP
                     GLENLALIPGCIGSSPIQNIGAYGVELQRVCAYVDCVELATGKQVRLTAKECRFGYRD
                     SIFKHEYQDRFAIVAVGLRLPKEWQPVLTYGDLTRLDPSTVTPQQVFDAVCHMRTTKL
                     PDPKVNGNAGSFFKNPVVSAETANALLAQFPTAPHYPQVDGSVKLAAGWLIDQCQLKG
                     TQIGGAAVHRQQALVLINEHDAKSEDVVQLAHHVRQKVGEKFNVWLEPEVRFIGASGE
                     VSAVETIS"
BASE COUNT         1100 a         1040 c          947 g         1131 t
ORIGIN      
        1 gcgtagtgac gggtcggggt gtcatattca acgtgagaag tgttgatggt gataccacga
       61 gctttttctt ccggcgcgtt atcgatctgg tcgaatgcgc gagcagcacc gccgtaggtt
      121 ttagccagta cggtagtgat tgcagcggtc agcgttgttt taccatggtc aacgtggccg
      181 atagtaccga cgttaacgtg cggttttgta cgttcaaact tttctttaga catcgattgt
      241 ccctctaaga cacggataaa tcggtgatat caccacatca accaggcaac atgcccgact
      301 tgttgaatgc aataaacaga aggaaaaaac agggaggaga aaagaagtgg tgctgatagg
      361 cagattcgaa ctgccgacct cacccttacc aagggtgcgc tctaccaact gagctatatc
      421 agcacatctt ggagcgggca gcgggaatcg aacccgcatc atcagcttgg aaggctgagg
      481 taatagccat tatacgatgc ccgcatcctg gaactcggct acctgatttt cattctgcac
      541 tgaatatcga gagaagctct ctttattcga gccggtaagc gaacttatcg tctcgggcta
      601 cgccatcgcg tggccgaaat tggtggtggg ggaaggattc gaaccttcga agtctgtgac
      661 ggcagattta cagtctgctc cctttggccg ctcgggaacc ccaccggact tgatggtgcc
      721 gactaccgga atcgaactgg tgacctactg attacaagtc agttgctcta cctactgagc
      781 taagtcggca tcaagtagcg cgcattctat ggagacatgc gagttcatgc aactaaaaaa
      841 ttgcataatt tgttttattg gtcacatttt atgcgacacg atgaagaaac agccgaacgg
      901 agggcagatt agcacacttt ttcaacatca ttgtgctcaa caatgtgctc ctgctaaacc
      961 ataattcttt ttatcagatg gaatatctgt cacattgctt ttcaacgata gcttcctggg
     1021 agagattttt tcttattatt cctccccatc tggtgttacc ctcctgccca ttaacccatt
     1081 caacagaact gtgacgcgcc atggcaaata tcgctttgcc gatagagcta tgaccgccag
     1141 aaacatgctt atgagtataa aagagcaaac gttaatgacg ccttacctac agtttgaccg
     1201 caaccagtgg gcagctctgc gtgattccgt acctatgacg ttatcggaag atgagatcgc
     1261 ccgtctcaaa ggtattaatg aagatctctc gttagaagaa gttgccgaga tctatttacc
     1321 tctgtcacgt ttgctgaact tctatataag ctcgaatctg cgccgtcagg cagttctgga
     1381 acagtttctt ggtaccaacg ggcaacgcat tccttacatt atcagtattg ctggtagtgt
     1441 cgcggtgggg aaaagtacaa ccgcccgtgt attgcaggcg ctattaagcc gttggccgga
     1501 acatcgtcgt gttgaactga tcactacaga tggcttcctt caccctaatc aggttctgaa
     1561 agaacgtggt ctgatgaaga agaaaggctt cccggaatcg tatgatatgc atcgcctggt
     1621 gaagtttgtt tccgatctca aatccggcgt gccaaacgtt acagcccctg tttactcgca
     1681 tcttatttat gatgtgatcc cggatggaga taaaactgtt gttcagcctg atattttaat
     1741 tcttgaaggg ttaaatgtct tacagagcgg gatggattat ccacacgatc cacatcatgt
     1801 atttgtttct gattttgtcg atttttcgat atatgttgat gcaccggaag acttacttca
     1861 gacgtggtat atcaaccgtt ttctgaaatt ccgcgaaggg gcttttacag acccggattc
     1921 ctattttcat aactacgcga aattaactaa agaagaagct attaagactg ccatgacatt
     1981 gtggaaagag atcaactggc tgaacttaaa gcaaaatatt ctacctactc gcgagcgtgc
     2041 cagtttgatc ctgacgaaaa gtgctaatca tgcagtagaa gaggtcagac tacgcaaata
     2101 attttcaggg gagctaatgc tcccctttct tatttttctg cactacgcag ggatatttca
     2161 ccgcccatcc agggttttat tattccatcc tgctcaagta ataaagcacc ctgtttgtct
     2221 attccgcgtg aaatgccaaa tatttcttta tcaccaatga taagtttcac tgggcgatta
     2281 ataaaattat ccaacttttc ccagcgcgaa agataaggag ccaatccttc ttgctcgaag
     2341 agttccagcg ccgcgcgtaa ttcacgtatc agcatggccg caagagtatt acgatcgaga
     2401 ttgatcccgg cttcctgcag cgtgatccac ccttgattaa cgacactctc ttcaacacga
     2461 cgcattgcca tgttgatccc tgcaccaatg actatttgcg ccgcatcgcc ggttttgcca
     2521 gtcagctcca caagaatgcc tgccagcttg cgatcctgca gatagaggtc attaggccat
     2581 ttaacacgaa ctttatctgc acccagcttg cgtaatactt ccgccatcac gataccgata
     2641 accagactta aaccaatcgc cgccgccggg ccctgttcca gacgccagaa catcgacaaa
     2701 tataagttcg cgccaaaagg cgaaaaccat ttccgaccac gacgaccacg gccagcatgc
     2761 tggtattctg caacacaggc atcgccagat ttaagctctc cgatgcggtc caggagatac
     2821 tgattcgtgg agtcaataac tggcagcacg gctacactgc cgtcatccag ctgactcaat
     2881 atcttttcgg cattaagtaa ctggattggc tcgggcaagc tgtatccttt acccggaaca
     2941 gtaaatacat caacgcccca gtcacgcagt gtctgaatgt gtttattaat agccgcccgg
     3001 ctcattccca gcgtttcacc caactgctcg ccagagtgaa attcgccatt cgctaacagg
     3061 gcgatcaatt tcagtggcac ggtgttatcc ttcatgaaat tgtctccact gcgctcactt
     3121 cacctgatgc accaataaag cggacttcag gctcaagcca gacattaaat ttttcaccca
     3181 ctttttgtct tacatgatgc gccagctgta caacatcttc gctttttgca tcgtgctcat
     3241 ttatgagaac cagcgcctgt tgacggtgta ccgcagcccc accaatttgc gtccctttaa
     3301 gctggcactg atcaataagc caacctgcag ccagttttac tgaaccatcc acctgggggt
     3361 aatgtggggc tgttggaaat tgtgccaaca acgcgttagc agtttcggca gaaacaacag
     3421 gatttttgaa gaaactaccc gcattaccgt tcacttttgg atcagggagt ttggtggtgc
     3481 gcatatgaca caccgcatca aatacttgct gtggcgttac cgttgaagga tccaggcgag
     3541 ttaagtcacc atacgtcagt acaggttgcc actcttttgg cagacgcaga cctacggcta
     3601 caatggcgaa gcgatcctgg tattcatgtt taaaaatact gtctcgatag ccaaaacggc
     3661 actctttggc agttaagcgt acttgcttgc ctgtcgccag ttcaacacaa tcaacataag
     3721 cgcaaactcg ctgtaattct acgccataag caccaatatt ctggataggt gatgagccga
     3781 tacaaccagg aattaatgcc agattttcca gaccaggcat accttcctgc aaagtgtatt
     3841 ttaccaggcg atgccagttt tctccggctc ctacatgtaa ataccacgca tcaggttcat
     3901 catgaatttc gatacctttg atccggttga tgatcaccgt gccgcgatag tcctccagaa
     3961 aaagtacatt acttccttca cccagaataa gaacgggttg tccttctgcg gttgcatgct
     4021 gccaggcatt gagtaattgt tgttcgtctt cggcacatac aatgtgctga gcattatgat
     4081 caatgccaaa tgtgttccag ggttttaagg agtggttcat agctgctttc ctgatgcaaa
     4141 aacgaggcta gtttaccgta tctgtggggg gatggcttgt agatatgacg acaggaagag
     4201 tttatagaaa cgcaaaaa
//
LOCUS       DADSYM010000219         4164 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2415
            SAMN08039579-rid11723173.denovo.221, whole genome shotgun sequence.
ACCESSION   DADSYM010000219 DADSYM010000000
VERSION     DADSYM010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039579
            Sequence Read Archive: SRR6362533
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4164)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4164)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980162.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 03:39:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,484
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 4,347
            CDSs (with protein)               :: 4,347
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 43 of 85
            Pseudo Genes (incomplete)         :: 37 of 85
            Pseudo Genes (internal stop)      :: 19 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4164
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039579-rid11723173.denovo.221"
                     /strain="LGE2415"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8302 N 95.9628 W"
                     /collection_date="2015-08-24"
     gene            complement(<1..1975)
                     /gene="speA"
                     /locus_tag="J1Y83_004153"
     CDS             complement(<1..1975)
                     /gene="speA"
                     /locus_tag="J1Y83_004153"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic arginine decarboxylase"
                     /protein_id="HBA7632237.1"
                     /translation="MSDDMSMGLPSSAGEHGVLRSMQEVAMSSQEASKMLRTYNIAWW
                     GNNYYDVNELGHISVCPDPDVPEARVDLAQLVKTREAQGQRLPALFCFPQILQHRLRS
                     INAAFKRARESYGYNGDYFLVYPIKVNQHRRVIESLIHSGEPLGLEAGSKAELMAVLA
                     HAGMTRSVIVCNGYKDREYIRLALIGEKMGHKVYLVIEKMSEIAIVLDEAERLNVVPR
                     LGVRARLASQGSGKWQSSGGEKSKFGLAATQVLQLVETLREAGRLDSLQLLHFHLGSQ
                     MANIRDIATGVRESARFYVELHKLGVNIQCFDVGGGLGVDYEGTRSQSDCSVNYGLNE
                     YANNIIWAIGDACEENGLPHPTVITESGRAVTAHHTVLVSNIIGVERNEYTVPTAPAE
                     DAPRALQSMWETWQEMHEPGTRRSLREWLHDSQMDLHDIHIGYSSGTFSLQERAWAEQ
                     LYLSMCHEVQKQLDPQNRAHRPIIDELQERMADKMYVNFSLFQSMPDAWGIDQLFPVL
                     PLEGLDQVPERRAVLLDITCDSDGAIDHYIDGDGIATTMPMPEYDPENPPMLGFFMVG
                     AYQEILGNMHNLFGDTEAVDVFVFPDGSVEVELSDEGDTVADMLQYVQLDPKTLLTQF
                     RDQVKKTDLDAELQQQFLEEFEAGLYGYTYLEDE"
     gene            complement(1984..2115)
                     /gene="yqgB"
                     /locus_tag="J1Y83_004154"
     CDS             complement(1984..2115)
                     /gene="yqgB"
                     /locus_tag="J1Y83_004154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417414.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid stress response protein YqgB"
                     /protein_id="HBA7632238.1"
                     /translation="MKKKPVAQLERQHSLLENPCAYGLLSQFQAAIVVNCFTLNKII"
     gene            2251..2466
                     /locus_tag="J1Y83_004155"
     CDS             2251..2466
                     /locus_tag="J1Y83_004155"
                     /inference="COORDINATES: protein motif:HMM:NF037550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA7632239.1"
                     /translation="MGITSAGMQSRDAECGERIFTRTVRQVKQQTTVHYFVSPPRPPV
                     KTNPQAKTLFSTRLEVATRKKRRVLFI"
     gene            2770..3924
                     /gene="metK"
                     /locus_tag="J1Y83_004156"
     CDS             2770..3924
                     /gene="metK"
                     /locus_tag="J1Y83_004156"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HBA7632240.1"
                     /translation="MAKHLFTSESVSEGHPDKIADQISDAVLDAILEQDPKARVACET
                     YVKTGMVLVGGEITTSAWVDIEEITRNTVREIGYVHSDMGFDANSCAVLSAIGKQSPD
                     INQGVDRADPLEQGAGDQGLMFGYATNETDVLMPAPITYAHRLVQRQAEVRKNGTLPW
                     LRPDAKSQVTFQYDDGKIVGIDAVVLSTQHSEEIDQKSLQEAVMEEIIKPILPAEWLT
                     SATKFFINPTGRFVIGGPMGDCGLTGRKIIVDTYGGMARHGGGAFSGKDPSKVDRSAA
                     YAARYVAKNIVAAGLADRCEIQVSYAIGVAEPTSIMVETFGTEKVPSEQLTLLVREFF
                     DLRPYGLIQMLDLLHPIYKETAAYGHFGREHFPWEKTDKAQLLRDAAGLK"
BASE COUNT         1043 a         1139 c         1013 g          969 t
ORIGIN      
        1 attcatcttc aagataggta taaccgtaca aacctgcctc gaactcttcc aggaactgtt
       61 gttgcagttc agcatcaaga tcggttttct tcacctgatc gcggaactgg gttaacagcg
      121 ttttcggatc gagctgcaca tattgcagca tgtccgccac ggtatcgcct tcgtcagaca
      181 gttccacttc tacactaccg tcagggaaga cgaacacgtc aaccgcttcg gtatcaccga
      241 acaggttgtg catgttgccg aggatctcct gatatgcgcc aaccataaag aaaccgagca
      301 tcggcggatt ctccggatcg tactccggca taggcattgt cgtggcaata ccgtcaccat
      361 caatatagtg gtcgatagca ccgtctgagt cacaggtaat atccagcagc acagcgcggc
      421 gttccggcac ttgatccagc ccttccagcg gcagaaccgg gaacaactgg tcgatccccc
      481 acgcatccgg catcgactgg aacagcgaga agttgacgta cattttgtcc gccatacgtt
      541 cctgcagctc gtcgataatc ggacgatgag cacggttttg cggatccagc tgcttttgca
      601 cttcatggca catgctcaaa taaagctgct cagcccatgc gcgttcttgc aggctaaagg
      661 tgccggaaga gtagccgata tgaatgtcgt gcagatccat ctggctgtcg tgtaaccatt
      721 cacgcagaga acggcgagtt cccggttcgt gcatttcctg ccaggtttcc cacatgcttt
      781 gcagggcgcg cggtgcgtct tctgcaggcg cggtcggcac cgtgtattcg ttacgttcca
      841 cgccgatgat attcgacacc agcacggtgt gatgcgcagt caccgcacga cccgattcgg
      901 tgattaccgt cggatgtggc agaccatttt cttcacacgc atcaccaatc gcccagatga
      961 tgttgttggc gtattcattg agtccgtagt ttacggagca gtcagactgc gaacgggtgc
     1021 cttcatagtc cacgcccaga ccgccgccga cgtcgaagca ctgaatattg acgcccagct
     1081 tgtgcagttc cacatagaaa cgcgcggatt cacgaacgcc tgtcgcgata tcgcgaatat
     1141 tcgccatctg cgaaccgagg tggaagtgca gcagttgcag gctgtcgaga cgcccggctt
     1201 cacgcagcgt ttcaaccagt tgcagtacct gagtcgccgc caggccaaac ttcgattttt
     1261 ccccgccgga ggactgccat ttacccgagc cctgcgaagc cagacgtgca cgcacgccca
     1321 gacgaggaac gacattcaga cgttctgctt catccagcac aatggcaatt tctgacatct
     1381 tctcaatgac cagatagacc ttgtggccca tcttctcgcc aattaatgcc aggcggatat
     1441 attcgcggtc cttataaccg ttgcagacga tgacgctacg ggtcatgcca gcatgtgcca
     1501 gcaccgccat caactcagct ttggaaccgg cttccaggcc cagcggttcg cccgaatgaa
     1561 tcagggactc aatcacgcgg cggtgctggt taactttgat cggataaacg aggaagtaat
     1621 cgccgttata gccgtaggat tcccgcgctc gtttgaacgc ggcgttaatg gaacgcaaac
     1681 ggtgctgcag gatctgtggg aaacagaaca gtgcaggcag acgctgaccc tgtgcttcgc
     1741 gagttttcac taactgcgcg agatcgacgc gagcttccgg gacgtccggg tccgggcaca
     1801 cgctgatgtg gcccagctcg ttaacgtcat agtagttatt gccccaccag gcaatattgt
     1861 aagtacgcag catcttgctg gcttcctggg agctcattgc aacctcctgc atggagcgta
     1921 gtacaccgtg ttcgcccgct gacgaaggca aacccataga catgtcgtca gacatagcga
     1981 acctcaaatt attttattaa gtgtaaaaca gttaacgact atcgcagcct ggaactgcga
     2041 taacaaccca taagcacatg gattttccag cagtgaatgc tgacgctcca actgcgcaac
     2101 cggtttcttt ttcataacat tattaagcac ataaccgaac gtaagtgtga cagttcggcg
     2161 aaaccacgag aaaactcttg tttttacaag agcgcccttg ttcagtcctc agtagctgta
     2221 accagctctt gaatcctgag aagcgccgag atgggtataa catcggcagg tatgcaaagc
     2281 agagatgcag agtgcgggga acgaatcttc accagaacgg tgagacaggt taagcagcag
     2341 acaacggttc attatttcgt atcacctcca cggccgcctg ttaagacgaa cccacaagcc
     2401 aaaactctgt tttcaacccg gctggaagtg gcaacacgaa agaaacgtcg tgtgcttttt
     2461 atttaagccg cgcgccgcgt tttatacccc acaatggcag aaaattgcaa aagataaata
     2521 cgcagaatgc cagcattgtc aggaaaaatt tccagccacg tttttaacag aatgagacac
     2581 gattcaaaaa aaagtggaaa ttgggtgaag aactgaccta aaatagccat ccagatgtta
     2641 atccatccat accgattaac actcagactg ccagtgtttt taacctgcag agtcgtggta
     2701 ggatccgcta ccacagaaaa tccacacaac agtttgagct aaccaaattc tctttaggtg
     2761 atattaaata tggcaaaaca cctttttacg tccgagtccg tctctgaagg gcatcctgac
     2821 aaaattgctg accaaatctc tgatgccgtt ttagacgcga tcctcgaaca ggatccgaaa
     2881 gcacgcgttg cttgcgaaac ctacgtaaaa accggcatgg ttttagttgg cggcgaaatc
     2941 accaccagcg cctgggtaga catcgaagag atcacccgta ataccgttcg cgaaattggc
     3001 tatgtgcatt ccgacatggg ctttgacgct aactcctgtg cggttctgag cgccatcggt
     3061 aaacagtctc ctgacatcaa ccagggcgtt gaccgtgccg atccgctgga acagggcgcg
     3121 ggcgaccagg gtctgatgtt tggctacgca actaatgaaa ccgacgtgct gatgccagca
     3181 cctatcactt atgcacaccg tctggtacag cgtcaggctg aagtgcgtaa aaacggcact
     3241 ctgccgtggc tgcgcccgga cgcgaaaagc caggtgactt tccagtatga cgacggcaaa
     3301 atcgttggca ttgatgctgt cgtgctttcc actcagcact ctgaagagat cgaccagaaa
     3361 tcgctgcaag aagcggtaat ggaagagatc atcaagccga ttctgcccgc tgaatggctg
     3421 acttctgcca ccaaattctt catcaacccg accggtcgtt tcgttatcgg tggcccgatg
     3481 ggtgactgcg gtctgactgg tcgtaaaatc atcgttgata cctacggcgg catggcgcgt
     3541 cacggtggcg gtgcattctc tggtaaagat ccatcaaaag tggaccgttc cgcagcctac
     3601 gcagcacgtt atgtcgcgaa aaacattgtt gctgctggcc tggccgatcg ttgtgaaatt
     3661 caggtttcct acgcaatcgg cgtggctgaa ccgacttcca tcatggtaga aactttcggt
     3721 actgagaaag tgccttctga acaactgact ctgctggtac gtgaattctt cgacctgcgc
     3781 ccatacggtc tgattcagat gctggatctg ctgcacccga tctacaaaga aaccgcagca
     3841 tacggtcact ttggtcgtga acatttcccg tgggaaaaaa ccgacaaagc gcagctgctg
     3901 cgtgatgctg ccggtctgaa gtaatctttc ttcacctgct ttcaaaggcc agcctcgcgc
     3961 tggccttttt cttttacctc accgcggtgg acaggcattc acgccgcatc cggcaaaaaa
     4021 accgaccgca caataacatc attcttcctc aacacgtttt accgcaaatt atcatcacta
     4081 ctgaaatcga ttacaccaac cacaccagcc aaatatttgt aatattttca tattattatt
     4141 cagctttcac agttgttaca tttc
//
LOCUS       DADSYM010000220         4160 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2415
            SAMN08039579-rid11723173.denovo.222, whole genome shotgun sequence.
ACCESSION   DADSYM010000220 DADSYM010000000
VERSION     DADSYM010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039579
            Sequence Read Archive: SRR6362533
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4160)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4160)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980162.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 03:39:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,484
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 4,347
            CDSs (with protein)               :: 4,347
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 43 of 85
            Pseudo Genes (incomplete)         :: 37 of 85
            Pseudo Genes (internal stop)      :: 19 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4160
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039579-rid11723173.denovo.222"
                     /strain="LGE2415"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8302 N 95.9628 W"
                     /collection_date="2015-08-24"
     gene            257..1006
                     /locus_tag="J1Y83_004157"
     CDS             257..1006
                     /locus_tag="J1Y83_004157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000039778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA7632241.1"
                     /translation="MSKKYQPLLITHYMSTWVTITEAVEITTKAIKQKITPSDIYRHA
                     LSGNILLSVYFQSPVILKKIQTFNGKIKFRQFVGDLLDKLCMLDRDGFIYGQNLRLCT
                     EARYICPVQQIIDPPLLRKLNQFRTFVRNVRPGDELDVPAQVSEKNLTPPPGNSSGNL
                     EQQIASTSQLIGSLLAEDMNSEQAANIARGWASSQASGVMTDWLSRFGTARITLGVDE
                     DFSLKNSRDGNPRKWRFDATNSELCAERHEC"
     gene            1139..2608
                     /locus_tag="J1Y83_004158"
     CDS             1139..2608
                     /locus_tag="J1Y83_004158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000646563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adhesin"
                     /protein_id="HBA7632242.1"
                     /translation="MKANFTLSDGDKAVTDADGKAKVTLKGTKAGAHTVTASMVGGKS
                     EQLVVNFTADTLTAQVNLNVTGDNFIANNIGMTRLQATVTDGNGNPVEGIKVNFRGTS
                     VTLSSTNVETDDQVFAEILVTSTEVGLKTVSASLADKPTEVISRLLNAKVDVNSATIT
                     SQEIPEGQVMVAQDIAVKAHVNDQFGNPVTHQPATFSAAPSSQMIISQNTVSTNTQGV
                     AEVTMTPERNGSYTVKASLANGASLEKQLEAIDEKLTLTSSPLIGVNAPKGATLTATL
                     TSANGTPVEGQVINFSVTLEGATLSGGKVRTNSSGQAPVVLTSNKVGTYTVTASFHNG
                     VTIQTQTTVKVTGNPSTAHVASFIADPSTIAATNSDLSTLKATVEDGSGNLIEGLTVY
                     FALKSGSTTLTSLTAVTDQNGIATTSVKGEITGSVTVSAVTSAGGMQTVDISLVAGPA
                     DASQSILKNNQSSLKGDFTDSAELHLVLHDISGNPIKVS"
     gene            2621..3097
                     /locus_tag="J1Y83_004159"
     CDS             2621..3097
                     /locus_tag="J1Y83_004159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001492130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="invasin"
                     /protein_id="HBA7632243.1"
                     /translation="MQSGTNVPYMKISAIDYSQNINGDYKATVTGGGEGIATLIPVLN
                     GVHQAGLSTTIEFISAETRPMTGTVSVNSANLPTASFPSQGFTGAYYQLNNDNFAPGK
                     TAADYSFSSSASWVGVDATGKVTFKNDGDSNTVIITAPPRSGGAIYQTVPPESRSV"
     gene            complement(3225..>4160)
                     /locus_tag="J1Y83_004160"
     CDS             complement(3225..>4160)
                     /locus_tag="J1Y83_004160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HBA7632244.1"
                     /translation="DAMAIFPVIRALELHKIKHLLIVTSTINLEVFNALKLEQTKLTL
                     VTMTMQDHATLKEIKDLAKNITLQYGTPDLCIEAMRKKNLKTMIFISQLKAKTNFQVV
                     DLTMKCYSPLCKNASRMDQNLRAPVPMTWAFMMREAGFPAVRSIYELPLSDDVLDEVR
                     EEMRSLGSYIALNLDGSSQERTFSLSIAENLIAKIQSETDIPIVIVYGPKGEDKARAL
                     VDCYNNVYRLSLSPSIKRSAAIIKDAYIAITPDTSILHMASAYNTPVVAIYADYKTRW
                     PAMADVSESVVVGQKIDNISLDEFAKALKSVLARI"
BASE COUNT         1226 a          957 c          915 g         1062 t
ORIGIN      
        1 atgcatacac aacacaaata tcactcaggt acactactca aaccacgctg ggatttttcc
       61 tcaagttata atcatcaccc cgaaaatcat tcggcattta ctcattaaat agtcacccca
      121 taggcctgta catgtttact cagaaatata catccttttc tctgtcataa accctctgat
      181 taatcataga taaatacttg tgacaccaat ctttttcctt aaaggaacga attgttgtgt
      241 agaaggagat aatattatgt caaaaaaata tcagccattg cttataactc attatatgtc
      301 aacatgggtc actataacgg aagcagttga gatcactacc aaagccataa aacaaaaaat
      361 tactcctagc gatatttatc gtcatgcctt gagtggcaac atcctactat cggtctattt
      421 ccagtctcct gtgatactta agaagataca aacttttaat ggaaaaataa aattcaggca
      481 gtttgtggga gacctacttg ataaactatg catgcttgac agggacggtt ttatttatgg
      541 tcaaaattta agactctgta cagaagccag atatatttgt cctgtgcaac aaattataga
      601 tccccccctg ttacgcaaac tcaaccagtt tcgtacgttt gttcgtaatg tccgcccggg
      661 tgatgaactg gatgtcccgg cacaagttag tgaaaaaaat ttaaccccgc cgccgggtaa
      721 cagcagtggc aaccttgagc aacagatagc cagtacttca cagctaattg gatctctgct
      781 cgccgaagat atgaacagcg agcaagcggc aaacatagca cgtggatggg cctcttctca
      841 ggcttcaggg gtaatgacag actggttaag ccgcttcggt accgcaagaa tcacgctggg
      901 cgtggatgaa gatttcagcc tgaagaactc ccgtgacggc aaccctcgaa aatggcgatt
      961 cgatgcaaca aacagtgaac tatgtgccga acgtcacgaa tgctgaaatc acgctggcag
     1021 cctcgaagga tccggtgatt gccgacaata acgatctcac gacactaaca gcaccgtcgc
     1081 tgatacagag ggcaatgcga tagccaacac tgaggtaaca tttactctac cggaagatgt
     1141 gaaggcgaac ttcacgctga gcgatggcga caaagcggtt actgatgccg atggcaaagc
     1201 gaaagtcacg ctgaaaggta caaaagcagg cgctcatacc gtgacagcat cgatggttgg
     1261 cggtaagagt gagcagttgg tggtgaactt tactgcggat acgctcactg cgcaggttaa
     1321 tcttaacgtt accggggaca atttcatcgc caataacatt ggaatgacca ggctacaggc
     1381 aacagtgact gatggaaacg gcaacccagt agaaggtata aaagttaatt tccgtggaac
     1441 ctccgtcacg ctaagcagca ccaacgttga aacggatgat caggttttcg ctgaaattct
     1501 tgtgacaagc accgaggtcg gactaaaaac agtttcagcc tctctggcag ataaacctac
     1561 tgaagtcatc tcgcgattac tgaatgccaa ggtagatgtt aattctgcga cgattaccag
     1621 tcaggagata cctgaaggtc aggtcatggt cgcacaagac atagcagtta aagctcacgt
     1681 taacgaccag tttggcaacc cggtgacgca tcaacccgcg acattcagtg cagcgccatc
     1741 ctcgcaaatg atcatcagcc agaatacggt ctctactaat acgcagggag tagccgaggt
     1801 caccatgacg cctgaaagaa acggctcgta tactgtaaaa gcatccctag cgaatggagc
     1861 ttcacttgag aaacaactgg aggctattga tgaaaaactg acactcacgt ccagtccgct
     1921 tatcggtgtc aatgctccta aaggcgctac tctgacggca acgctaacct ctgcaaatgg
     1981 cactccagtg gagggtcagg tcatcaactt tagcgtaacg ctagaaggtg cgacgttaag
     2041 tggcggaaaa gtgagaacca actcttcagg tcaggctcca gtcgttctga ccagcaataa
     2101 agtcggtaca tatacggtga ctgcatcgtt ccataacggc gtcacaatac agacacagac
     2161 aaccgtgaag gttactggca acccaagcac cgcacatgtt gctagcttta tcgctgatcc
     2221 gtcgactatc gccgccacca acagtgattt aagtacctta aaggcaacgg ttgaggatgg
     2281 cagtggtaac ctgatcgaag gtctcactgt gtacttcgcc ttaaaaagcg gctccaccac
     2341 attaacgtca ttaacagcgg tgaccgatca aaacggaatc gcgacaacaa gcgtgaaagg
     2401 tgagataacg gggagcgtca ctgtaagcgc agtcacaagc gcaggtggaa tgcaaacagt
     2461 agatatatcg ctagtggcag gcccagcaga cgcctcgcag tccatcctta agaacaacca
     2521 gtcatcactg aaaggggact ttaccgatag tgctgagcta catcttgttc tgcacgatat
     2581 atcaggcaat ccgatcaaag tttcttaagg gatagaattt gtgcaatcag gtacaaacgt
     2641 gccctatatg aaaattagcg caattgatta cagtcaaaat atcaacggtg attacaaagc
     2701 tactgttaca ggaggcggag agggtatcgc aacgctgatc cccgtattga atggtgttca
     2761 tcaggcgggt ctgagtacca cgatagaatt cattagcgcg gaaaccagac caatgacggg
     2821 tacagtgtca gtgaatagtg caaacctacc gacagcttca ttcccttcgc agggatttac
     2881 cggggcgtat tatcagttga ataatgacaa ctttgcacca ggaaaaacgg cggctgatta
     2941 ttcgttttca agctcggcct cctgggtagg cgttgatgct actggtaaag tgacattcaa
     3001 aaatgacggt gatagcaata ctgtaataat cacagcgccc ccaagatccg gtggtgctat
     3061 ttaccaaaca gttcctcctg aatcgcgttc ggtgtgacta tgatcatttt cggtgagtat
     3121 tgctttgatc caaaggctgg ttttctgtga tgattgacca gcctcagagt attgatgaca
     3181 ccaccgttca taacagatcg gcattactat gcataagtac tgcctcaaat tctcgccaaa
     3241 acacttttta atgcctttgc gaattcatcc agacttatat tgtcaatttt ttgcccaacg
     3301 acgactgact ccgaaacatc ggccattgcg ggccatcgcg ttttgtaatc agcataaatt
     3361 gcaacaacag gggtattata ggcacttgcc atatgtaata ttgaggtgtc aggagttatt
     3421 gcaatataag catcttttat gattgctgct gaacgtttaa tcgagggtga taaggataaa
     3481 cggtagacat tattataaca atccactaat gccctggctt tatcttcccc tttgggtcca
     3541 taaacgatca ctattggaat atctgtttca ctttgaattt ttgctattag attttctgca
     3601 atcgataatg aaaatgtacg ttcctgcgag ctaccgtcta aattgagcgc aatgtaagat
     3661 cctaacgatc gcatttcctc gcgtacctca tcgagtacat catcgcttaa tggcaattca
     3721 tatattgacc tgactgccgg aaaacccgcc tcacgcatca taaatgccca tgtcatgggt
     3781 acaggagccc ggagattctg atccatcctg gacgcgttct tacacaaagg ggaatagcac
     3841 ttcatggtta aatcgacgac ctggaaattc gtttttgctt tcaactgact gataaatatc
     3901 atcgttttca ggttcttttt acgcatcgcc tctatacaaa gatccggcgt accgtattgc
     3961 agtgttatgt tcttcgctaa atcttttatt tcttttaatg ttgcgtgatc ctgcatagtc
     4021 attgtgacta atgttaattt agtctgttca agtttgagcg cattaaagac ttctaaatta
     4081 attgtcgacg taacaattaa tagatgctta attttatgca attcaagcgc ccgaataaca
     4141 ggaaagatgg ccatagcatc
//
LOCUS       DADSYM010000221         4159 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2415
            SAMN08039579-rid11723173.denovo.223, whole genome shotgun sequence.
ACCESSION   DADSYM010000221 DADSYM010000000
VERSION     DADSYM010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039579
            Sequence Read Archive: SRR6362533
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4159)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4159)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980162.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 03:39:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,484
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 4,347
            CDSs (with protein)               :: 4,347
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 43 of 85
            Pseudo Genes (incomplete)         :: 37 of 85
            Pseudo Genes (internal stop)      :: 19 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4159
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039579-rid11723173.denovo.223"
                     /strain="LGE2415"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8302 N 95.9628 W"
                     /collection_date="2015-08-24"
     gene            107..4048
                     /gene="vat"
                     /locus_tag="J1Y83_004161"
                     /pseudo
     CDS             107..4048
                     /gene="vat"
                     /locus_tag="J1Y83_004161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021534952.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="vacuolating autotransporter toxin Vat"
BASE COUNT         1144 a          958 c         1064 g          993 t
ORIGIN      
        1 agggagtacc cgcagaggaa aaagactttc agtacttacc tctctggcac tatctgcatt
       61 actcccaacc gttgctggtg catcaacggt tggtggcaac aatccttacc agacataccg
      121 cgactttgca gaaaacaaag ggcagtttca ggctggcgca acaaacattc ctatttttaa
      181 taataaaggg gaattagtag gacatcttga taaagcgccc atggttgatt ttagcagtgt
      241 gaatgtaagc tcaaatcccg gcgttgcaac attaattaac ccgcaatata tagccagtgt
      301 aaaacataat aaaggatatc agagcgtcag cttcggtgat ggtcagaaca gttaccatat
      361 tgtggatcgt aatgaacaca gttcatctga tctccacaca ccaagacttg ataagctcgt
      421 aactgaggtt gctccggcta ccgtaaccag ctcatcaaca gctgatatat tgaacccttc
      481 aaaatactcg gcattctaca gggctggttc gggaagtcag tatattcagg atagtcaggg
      541 taagcgacat tgggtaacag gtgggtatgg ttatctgaca ggaggaatac tcccgacatc
      601 attcttttat cacggctcag acggcattca gctgtatatg gggggcaaca tacatgatca
      661 tagcatcctg ccctcttttg gagaggccgg cgacagtggt tctccattat ttggctggaa
      721 tacggccaaa gggcagtggg aactggtcgg tgtttactcg ggagtaggag gggggaccaa
      781 tttgatatat tctcttattc ctcagagttt tctctcacag atctattcag aggataatga
      841 cgctcccgtc ttttttaatg cctcatccgg cgcccccctg caatggaaat ttgacagcag
      901 caccggcact ggctctctga aacagggttc cgatgaatat gccatgcacg ggcaaaaagg
      961 ttctgacctg aacgcaggta aaaatctgac attcctggga cataatggtc agattgacct
     1021 ggaaaactct gtcacgcagg gtgccggttc actgacattt actgatgact acactgtcac
     1081 cacttcaaac ggaagtacct ggaccggggc cggtattatt gtggacaagg atgcctccgt
     1141 aaactggcag gttaatggtg tgaaaggtga caacctgcat aaaatcggcg aaggaaccct
     1201 ggttgtacag ggaaccggtg ttaatgaggg cggcctgaaa gtcggggatg ggaccgttgt
     1261 cctcaatcag caggctgaca gttcaggaca cgttcaggca ttcagtagcg tgaatattgc
     1321 cagcggccgc ccgacagtcg tgctggcaga caaccagcag gttaatccgg acaatatatc
     1381 ctggggctac cggggggggg ttctggatgt taacgggaat gacctgacat ttcataagct
     1441 gaatgccgcc gattatggcg caactctcgg taacagcagt gataaaacgg ctaatatcac
     1501 tctggattat cagacgcatc cggcagacgt aaaagttaat gaatggtcat catcaaacag
     1561 gggaacagta ggttcattat atatttataa taatccctat actcataccg tcgattattt
     1621 tatcctgaaa acaagtagtt atggctggtt ccctaccggt caggtcagta acgagcactg
     1681 ggaatatgtc ggacatgacc agaacagtgc acaggcactg cttgcaaaca gaattaataa
     1741 taaagggtat ctgtatcatg gcaagttgct gggaaatatt aatttctcaa ataaagcaac
     1801 cccgggtaca accggcgcat tggttatgga cggctcagcg aatatgtccg gtacatttac
     1861 tcaggaaaac ggtcgtctga ccattcaggg ccacccggtt atccatgctt caacgtctca
     1921 gagtattgca aatacagtct cgtctctggg cgacaattcc gttctgacac agcccacctc
     1981 atttacacag gatgactggg agaacaggac gttcagcttt ggttcgctcg tgttaaaaga
     2041 tacagacttt ggtctgggcc gcaatgccac actgaacaca accatccagg cagataactc
     2101 cagcgtcacg ctgggcgaca gtcgggtatt tatcgacaaa aaagatggcc agggaacagc
     2161 atttaccctt gaagaaggca catctgttgc aactaaagat gcagataaaa gcgtcttcaa
     2221 cggcaccgtc aacctggata atcagtcagt gctgaatatc aatgagatat tcaatggcgg
     2281 aatacaggcg aacaacagta ccgtgaatat ctcctcagac agtgccgttc tggagaactc
     2341 aacgctgacc agtaccgccc tgaatctgaa caagggagca aatgttctgg ccagtcagag
     2401 ttttgtttct gacggtccgg tgaatatttc tgatgccacc ctgagtctga acagccgtcc
     2461 tgatgaggta tctcacacac ttttacctgt atacgattat gccggttcat ggaacctgaa
     2521 gggagacgat gcccgcctga acgtggggcc gtacagtatg ttgtcaggta atatcaatgt
     2581 tcaggataaa gggactgtca ccctcggagg ggaaggggaa ctgagtcctg acctgactct
     2641 tcagaatctg atgttgtaca gcctgtttaa cgggtaccgc aatacctgga gcgggagcct
     2701 gaatgcaccg gatgccaccg tcagcatgac agacacccag tggtcgatga acggaaactc
     2761 cacggcagga aatatgaaac ttaaccggac aatagtcggt tttaacgggg gaacatcatc
     2821 gttcacgaca ctgacaacag ataatctgga cgcggttcag tcagcatttg tcatgcgtac
     2881 agaccttaac aaggcagaca aactggtgat aaacaagtcg gcaacaggtc atgacaacag
     2941 catctgggtt aacttcctga aaaaaccctc tgacaaggac acgcttgata ttccactggt
     3001 cagcgcacct gaagcgacag ctgataatct gttcagggca tcaacacggg ttgtgggatt
     3061 cagtgatgtc acccccaccc ttagtgtcag aaaagaggac gggaaaaaag agtgggtcct
     3121 cgatggttac caggttgcac gtaacgacgg ccagggtaag gctgccgcca cattcatgca
     3181 catcagctat aacaacttca tcactgaagt taacaacctg aacaaacgca tgggcgattt
     3241 gagggatatt aacggcgaag ccggtacgtg ggtgcgtctg ctgaacggtt ccggctctgc
     3301 tgatggcggt ttcactgacc actataccct gctgcagatg ggggctgacc gtaagcacga
     3361 actgggaagt atggacctgt ttaccggcgt gatggccacc tacactgaca cagatgcgtc
     3421 agcaggcctg tacagcggta aaacaaaatc atggggtggt ggtttctatg ccagtggtct
     3481 gttccggtcc ggcgcttact ttgatttgat tgccaaatat attcacaatg aaaacaaata
     3541 tgacctgaac tttgccggag ctggtaaaca gaacttccgc agccattcac tgtatgcagg
     3601 tgcagaagtc ggataccgtt atcatctgac agatacgacg tttgttgaac ctcaggcgga
     3661 actggtctgg ggaagactgc agggccaaac atttaactgg aacgacagtg gaatggatgt
     3721 ctcaatgcgt cgtaacagcg ttaatcctct ggtaggcaga accggcgttg tttccggtaa
     3781 aaccttcagt ggtaaggact ggagtctgac agcccgtgcc ggcctgcatt atgagttcga
     3841 tctgacggac agtgctgacg ttcacctgaa ggatgcagcg ggagaacatc agattaatgg
     3901 cagaaaagac ggtcgtatgc tttacggtgt ggggttaaat gcccggtttg gcgacaatac
     3961 gcgtctgggg ctggaagttg aacgctctgc attcggtaaa tacaacacag atgatgcgat
     4021 aaacgctaat attcgttatt cattctgatg ctgtgcagca aagggactat tgctttgtcc
     4081 ctttgctgcg attatctgat tacggtacag gctggaactt agattttccg gcgttcacga
     4141 tacttcatgt aacactcag
//
LOCUS       DADSYM010000222         4052 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2415
            SAMN08039579-rid11723173.denovo.224, whole genome shotgun sequence.
ACCESSION   DADSYM010000222 DADSYM010000000
VERSION     DADSYM010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039579
            Sequence Read Archive: SRR6362533
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4052)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4052)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980162.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 03:39:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,484
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 4,347
            CDSs (with protein)               :: 4,347
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 43 of 85
            Pseudo Genes (incomplete)         :: 37 of 85
            Pseudo Genes (internal stop)      :: 19 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4052
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039579-rid11723173.denovo.224"
                     /strain="LGE2415"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8302 N 95.9628 W"
                     /collection_date="2015-08-24"
     gene            4..1662
                     /gene="dpiB"
                     /locus_tag="J1Y83_004162"
     CDS             4..1662
                     /gene="dpiB"
                     /locus_tag="J1Y83_004162"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase DpiB"
                     /protein_id="HBA7632245.1"
                     /translation="MLQLKENKQFAFFQRLAFPLRIFLLILVFSIFVIAALAQYFTAS
                     FEDYLTLHVRDMAMNQAKIIASNDSIISAVKTRDYKRLATIADKLQRDTDFDYVVIGD
                     RHSIRLYHPNPEKIGYPMQFTKPGALEKGESYFITGKGSIGMAMRAKTPIFDDDGKVI
                     GVVSIGYLVSKIDSWRAEFLLPMAGVFVVLLGILMLLSWFLAAHIRRQMMGMEPKQIA
                     RVVRQQEALFSSVYEGLIAVDPHGYITAINRNARKMLGLSSPGRQWLGKPIAEVVRPA
                     DFFTEQIDEKRQDVVANFNGLSVIANREAIRSGDDLLGAIISFRSKDEISTLNAQLTQ
                     IKQYVESLRTLRHEHLNWMSTLNGLLQMKEYDRVLAMVQGESQAQQQLIDSLREAFAD
                     RQVAGLLFGKVQRARELGLKMVIVPGSQLSQLPPGLDSTEFAAIVGNLLDNAFEASLR
                     SDEGNKIVELFLSDEGDDVVIEVADQGCGVPESLRDKIFEQGVSTRADEPGEHGIGLY
                     LIASYVTRCGGVITLEDNDPCGTLFSIYIPKVKPNDSSINPIDR"
     gene            1631..2311
                     /gene="dpiA"
                     /locus_tag="J1Y83_004163"
                     /gene_synonym="citB"
     CDS             1631..2311
                     /gene="dpiA"
                     /locus_tag="J1Y83_004163"
                     /gene_synonym="citB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component response regulator DpiA"
                     /protein_id="HBA7632246.1"
                     /translation="MTAPLTLLIVEDETPLAEMHAEYIRHIPGFSQILLAGNLAQARM
                     MIERFKPGLILLDNYLPDGRGINLLHELVQAHYPGDVVFTTAASDMETVSEAVRCGVF
                     DYLIKPIAYERLGQTLTRFRQRKHMLESIDSASQKQIDEMFNAYARGEPKDELPTGID
                     PLTLNAVRKLFKEPGVQHTAETVAQALTISRTTARRYLEYCASRHLIIAEIVHGKVGR
                     PQRIYHSG"
     gene            complement(2352..3737)
                     /gene="dcuC"
                     /locus_tag="J1Y83_004164"
     CDS             complement(2352..3737)
                     /gene="dcuC"
                     /locus_tag="J1Y83_004164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuC"
                     /protein_id="HBA7632247.1"
                     /translation="MLTFIELLIGVVVIVGVARYIIKGYSATGVLFVGGLLLLIISAI
                     MGHKVLPSSQASTGYSATDIVEYVKILLMSRGGDLGMMIMMLCGFAAYMTHIGANDMV
                     VKLASKPLQYINSPYLLMIAAYFVACLMSLAVSSATGLGVLLMATLFPVMVNVGISRG
                     AAAAICASPAAIILAPTSGDVVLAAQASEMSLIDFAFKTTLPISIAAIIGMAIAHFFW
                     QRYLDKKEHISHEMLDVSEITTTAPAFYAILPFTPIIGVLIFDGKWGPQLHIITILVI
                     CMLIASILEFIRSFNTQKVFSGLEVAYRGMADAFANVVMLLVAAGVFAQGLSTIGFIQ
                     SLISIATSFGSASIILMLVLVILTMLAAVTTGSGNAPFYAFVEMIPKLAHSSGINPAY
                     LTIPMLQASNLGRTLSPVSGVVVAVAGMAKISPFEVVKRTSVPVLVGLVIVIVATELM
                     VPGTAAAVTGK"
BASE COUNT         1106 a          943 c         1041 g          962 t
ORIGIN      
        1 acaatgttgc agcttaaaga gaataaacag tttgcatttt tccaaagact ggcatttccg
       61 ctgcgtatct ttttgctgat tctggtgttc tcaatatttg tcattgcagc cctggcgcaa
      121 tattttacag ccagttttga ggactattta acgcttcatg tgcgcgacat ggcaatgaat
      181 caggcaaaaa ttattgcttc taatgacagt atcatcagtg cagtgaaaac gcgtgactac
      241 aagaggctgg cgaccatcgc tgacaaatta caaagagata ctgatttcga ttatgtggtg
      301 attggcgatc ggcactcgat tcgtctttac catcctaatc cggagaaaat tggttatcct
      361 atgcagttca ccaaaccggg cgcgctggag aaaggggaga gctacttcat caccgggaaa
      421 gggtcaattg gcatggcgat gcgtgccaaa acgccaatct ttgatgacga tggaaaagtc
      481 atcggcgtgg tgtcgattgg ctatctggtg agtaaaatcg atagctggcg ggctgagttt
      541 ttattaccga tggcaggcgt gtttgtcgtg ctgttaggga ttttgatgtt gctgtcgtgg
      601 ttcctggccg cgcatatccg tcggcagatg atgggcatgg agccaaagca aatcgctcgc
      661 gtagtccgtc agcaagaggc gctgtttagt tcggtttatg aagggctgat tgcggtggat
      721 ccgcacgggt acattaccgc catcaatcgt aacgcaagaa agatgctggg gctgagttcc
      781 cccggacggc aatggttggg taaacccatt gctgaagtgg tcaggcccgc cgatttcttt
      841 accgaacaga ttgatgaaaa acgtcaggat gtggtggcga actttaacgg tctgagcgtt
      901 attgccaacc gggaagctat tcgttctggc gatgatttac tgggggccat tattagcttt
      961 cgtagtaaag acgaaatttc caccctcaat gcgcaactga cgcaaattaa acaatacgtc
     1021 gagagccttc gtacattgcg acacgagcat ctcaattgga tgtcgacgct caatggtctg
     1081 ttgcagatga aagagtatga tcgcgtgctg gcgatggtgc agggggagtc tcaggcccag
     1141 caacagctta ttgacagcct gcgtgaggcg tttgccgatc gccaggtggc ggggctgctt
     1201 tttggtaaag tgcagcgcgc ccgcgaactg gggctaaaaa tggtcatcgt ccccggtagc
     1261 cagctttcgc aactgccgcc aggactggac agcaccgagt ttgcagccat tgttggcaat
     1321 ttacttgata acgccttcga agcaagcctg cgtagcgatg aaggaaacaa gatcgttgaa
     1381 ttattcctca gcgatgaagg cgatgatgtg gtgattgaag tcgccgatca gggctgcggc
     1441 gttccagagt ctctacgaga caaaatattt gagcagggtg tcagtacgcg tgctgacgag
     1501 cccggtgaac atggcattgg gttgtacttg attgccagct acgtaacgcg ctgcggtggt
     1561 gttatcactc tcgaagataa tgatccctgc ggtaccttat tttcaatcta tattccgaaa
     1621 gtgaaaccta atgacagctc cattaaccct attgatcgtt gaggacgaaa cgccgctggc
     1681 agagatgcat gcggaatata ttcgtcacat tcccggattc agccagatat tactggcggg
     1741 aaacctggcg caggcccgaa tgatgatcga gcgttttaag ccggggctaa tcttgctcga
     1801 taactatctt cctgacggta gagggattaa tttactgcat gaactggtgc aggcgcatta
     1861 tcccggcgac gtggtgttta ccactgctgc cagcgatatg gaaacggtgt ctgaagccgt
     1921 acgttgtggt gtatttgatt atctcattaa gccaattgcc tatgaacggt tggggcaaac
     1981 gctaacccgt ttccgccagc gtaaacatat gctggaaagt attgatagcg ccagccagaa
     2041 gcaaattgat gagatgttta atgcctatgc tcgcggtgaa cccaaagacg aactgccgac
     2101 cggcattgac cccttgacgc taaacgcggt gcgaaaactg ttcaaagagc ctggtgtgca
     2161 acatacggca gaaacggtgg cgcaggcact gaccatcagt cgcaccactg ccaggcgtta
     2221 tcttgaatat tgcgccagcc gccatctgat tattgctgaa attgttcacg gcaaagttgg
     2281 cagaccgcaa cgcatatacc acagtgggtg acatgataaa gccggaggaa acttgcctcc
     2341 ggcattacta tttacttgcc tgtgaccgct gctgccgttc ctggcaccat cagctctgta
     2401 gcaacgataa caatcaccaa accaacaagc accggcaccg aggtgcgttt tacgacttca
     2461 aacggcgaga tcttcgccat cccggcaacc gcaacgacta cgccagaaac gggtgatagg
     2521 gtacgaccca ggtttgacgc ctgcagcatc gggatagtca aatacgccgg gttaatgccg
     2581 gaggagtgcg ccagtttcgg gatcatctca acaaacgcat aaaacggcgc attgcctgaa
     2641 ccggtcgtga ctgccgccag cattgtcagg atcaccaata ccagcatcag gatgatactc
     2701 gccgaaccaa acgaggtagc gatagaaatc agactttgaa taaagccgat ggtgctaagc
     2761 ccctgagcga ataccccagc ggcaaccagc agcatcacca cgttagcaaa tgcatctgcc
     2821 ataccgcgat aagccacttc cagaccagag aaaactttct gggtattaaa gctgcggatg
     2881 aactccagaa tggaggcaat tagcatacaa atcaccagaa tagtgatgat gtgtaattgc
     2941 ggaccccatt tgccgtcaaa aatcagcact ccgatgatcg gcgtgaacgg caaaatggca
     3001 taaaacgcag gggcagtggt ggtgatttca ctgacatcta acatttcatg agagatgtgc
     3061 tcttttttat ccagataacg ttgccagaag aagtgggcga ttgccatgcc gataattgca
     3121 gcaattgaga taggcagcgt tgttttgaag gcgaagtcaa tcagcgacat ttcggaagcc
     3181 tgcgccgcca gcaccacatc ccctgaagtc ggtgcgagaa taatcgccgc cggggaggca
     3241 caaatggcag cagctgcgcc acgactgata ccaacgttta ccatcaccgg aaacagggtt
     3301 gccatcagca aaacacccag accggttgcg gaagagacgg ccagtgacat cagacaggca
     3361 acaaaatagg cggcaatcat cagaaggtaa ggggagttaa tatactgcaa tggttttgac
     3421 gccagcttga ccaccatatc attcgcgccg atatgggtca tgtaagcggc aaagccacac
     3481 agcatcataa tcatcatgcc gaggtcgcca ccgcggctca ttagcaatat tttaacgtat
     3541 tcaacgatat ccgtggcgct gtagcctgtt gaagcctggc tggacggtaa cactttgtgc
     3601 cccataatgg cactgataat cagcaataac aggccaccga caaataacac gccagtggcc
     3661 gaataccctt taatgatgta gcgagctaca cccacaataa ccacaacccc aataaggagt
     3721 tcaatgaatg tcagcataat ttttcctgtc tccaggcccc aaagtaaata ataaaaaatt
     3781 cctaaaagtt aaggaaaaaa tatgcccaat aaattggcgg tgaatgctga ttaaaatcaa
     3841 gaaaaactgc cattaagaca ttgaagttgc tgtttttatg cgtagataac aaaacgcata
     3901 tttagcggtt ggcaaatatc acataataat gtgtgtaaat tgatggtgga ttgatgcaaa
     3961 tttgttaatt gtaaggtgtt agctgcggtt gtgtgctcaa aaaataatct tccatgagca
     4021 tgagttgacg atacataata tatttttcgt ta
//
LOCUS       DADSYM010000223         3881 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2415
            SAMN08039579-rid11723173.denovo.225, whole genome shotgun sequence.
ACCESSION   DADSYM010000223 DADSYM010000000
VERSION     DADSYM010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039579
            Sequence Read Archive: SRR6362533
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3881)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3881)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980162.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 03:39:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,484
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 4,347
            CDSs (with protein)               :: 4,347
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 43 of 85
            Pseudo Genes (incomplete)         :: 37 of 85
            Pseudo Genes (internal stop)      :: 19 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3881
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039579-rid11723173.denovo.225"
                     /strain="LGE2415"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8302 N 95.9628 W"
                     /collection_date="2015-08-24"
     gene            complement(<1..300)
                     /gene="escO"
                     /locus_tag="J1Y83_004165"
     CDS             complement(<1..300)
                     /gene="escO"
                     /locus_tag="J1Y83_004165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE ATPase activity
                     positive regulator EscO"
                     /protein_id="HBA7632248.1"
                     /translation="MAKINSQIKEVDGKLDDCEQSIKESIASKQAYCASLVNLDKVSL
                     YKYQIKNNAFDEQKQRLYEKKSSLSKEKRSLLDSQKRTKENLQHVNKSVEKLSFAI"
     gene            complement(360..1700)
                     /gene="escN"
                     /locus_tag="J1Y83_004166"
     CDS             complement(360..1700)
                     /gene="escN"
                     /locus_tag="J1Y83_004166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE ATPase EscN"
                     /protein_id="HBA7632249.1"
                     /translation="MISEHDSVLEKYPRIQKVLNSTVPALSLNSSTRYEGKIINIGGT
                     IIKARLPKARIGAFYKIEPSQRLAEVIAIDEDEVFLLPFEHVSGMYCGQWLSYQGDEF
                     KIRVGDALLGRLVDGIGRPMESNIAAPYLPFERSLYAEPPDPLLRQVIDQPFILGVRA
                     IDGLLTCGIGQRIGIFAGSGVGKSTLLGMICNGASADIIVLALIGERGREVNEFLALL
                     PQSTLSKCVLVVTTSDRPALERMKAAFTATTIAEYFRDQGKNVLLMMDSVTRYARAAR
                     DVGLASGEPDVRGGFPPSVFSSLPKLLERAGPAPKGSITAIYTVLLESDNVNDPIGDE
                     VRSILDGHIVLTRELAEENHFPAIDIGLSASRVMHNVVTSEHLRAAAECKKLIATYKN
                     VELLIRIGEYTMGQDPEADKAIKNRKLIQNFIQQSTKDISSYEKTIESLFKVVA"
     gene            complement(1684..3711)
                     /gene="escV"
                     /locus_tag="J1Y83_004167"
     CDS             complement(1684..3711)
                     /gene="escV"
                     /locus_tag="J1Y83_004167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE export apparatus
                     protein EscV"
                     /protein_id="HBA7632250.1"
                     /translation="MNKLLNIFKKAESYHDLILALFFFMAVMMMIIPLPTVVVDIIIA
                     INISTALLLLMLSIYIKNPLELTSFPTILLITTLMRLSLSVSTTRLILLHHDAGDIIY
                     SFGNFVVGGNIVVGLVIFTIITIVQFMVITKGAERVAEVSARFSLDGMPGKQMSIDGD
                     MRAGVIDPLEAKVLRSRVQKESQFYGSMDGAMKFVKGDAIAGIIIVLVNLFGGVLIGM
                     WQFDMPFSEALSLFSVLSVGDALVAQIPALIISVTAGVVVTRVPGESEKEENLAGDIV
                     QQVSVNSRPFLISAALMLVMAIIPGFPTLVFLFLAVCLLGIAWKLQKKRTFGTGNNKD
                     AMGADLSNSQNISPGAEPLILNLSSNIYSSDITQQIEVMRWNFFEESGIPLPKIIVNP
                     VKNNDSAIEFLLYQESIYKDTLIDDTVYFEAGHAEISFEFVQEKLSTNSIVYKTNKAN
                     QQLAHLTGMDVYATTNDKITFLLKKLVLSNAKEFIGVQETRYLMDIMERKYNELVKEL
                     QRQLGLSKIVDILQRLVEENVSIRDLRTIFETLIFWSTKEKDVVILCEYVRIALRRHI
                     LGRYSVSGTLLNVWLIGSDIENELRESIRQTSSGSYLNISPERTEQIIGFLKNIMNPT
                     GNGVILTALDIRRYVKKMIEGSFPSVPVLSFQEVGNNIELKVLGTVNDFRA"
     gene            complement(3708..>3881)
                     /gene="cesL"
                     /locus_tag="J1Y83_004168"
     CDS             complement(3708..>3881)
                     /gene="cesL"
                     /locus_tag="J1Y83_004168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE chaperone CesL"
                     /protein_id="HBA7632251.1"
                     /translation="FFSALAPERTQGVIEHIFVYDNKLCLSCLLTNIDVFFLMNTFQQ
                     HVQIIERVRRMTS"
BASE COUNT         1187 a          895 c          708 g         1091 t
ORIGIN      
        1 tatggcaaaa gataattttt ctactgattt attaacatgt tgcagattct cttttgttcg
       61 tttttgtgaa tctagcaatg aacgcttttc cttcgaaagt gaagattttt tctcatacaa
      121 tcgctgcttt tgctcatcaa atgcattgtt tttaatctga tatttataca acgacacttt
      181 atccaaattc accagcgatg cacaatatgc ctgcttagag gcaatcgact ctttaatgga
      241 ttgctcgcag tcatcgagtt taccatccac ctcttttatc tgagagttaa tcttagccat
      301 tgattctctc aatcggttcg ctctgctttt acgaatagat aaaattctgt ccaacatact
      361 caggcaacca ctttgaatag gctttcaatc gttttttcgt aactactgat atctttggtg
      421 ctttgttgta taaagttctg aattaatttc ctatttttta ttgccttatc tgcttcagga
      481 tcttgcccca tcgtgtactc accaatacga ataagcagct caacattttt ataagttgca
      541 ataagctttt tgcattctgc cgccgcacgc aaatgctccg atgtaacaac gttatgcata
      601 acacgactgg cacttaaacc aatatcaatt gcagggaaat ggttttcctc tgcaagttct
      661 cgggtaagta cgatgtgccc atcaagaata gagcggactt catcgccaat aggatcatta
      721 acattatcgc tttctaacaa caccgtataa atcgcggtta ttgaaccttt tggcgcaggc
      781 cccgctcgct ctaataattt gggtaacgaa gaaaaaacac tcggagggaa tccccccctt
      841 acatcaggtt ctcccgatgc aagcccaaca tcacgtgcgg ctcgggcata acgagttaca
      901 gaatccatca ttaataatac atttttacct tgatcgcgga agtactctgc aatagtcgtc
      961 gccgtaaatg cggctttcat cctttccagc gcggggcggt ctgacgttgt gacgaccagt
     1021 acacatttag aaagcgtgga ttgaggtaag agcgcgagga attcatttac ttcgcgacca
     1081 cgttcgccga taagagcaag gacaataata tctgcggatg cgccattaca aatcatcccc
     1141 aaaagcgtac ttttaccaac gcctgaacca gcaaaaatac cgatacgctg cccaataccg
     1201 caagttagta gcccatcaat tgccctgacg ccaagtataa atggctgatc aataacctgc
     1261 cttaataaag gatctggtgg ttcagcatac aagctgcgtt caaacggaag atagggggca
     1321 gcaatattac tctccattgg tctgcctatg ccatcaacaa gccgcccaag tagtgcgtct
     1381 ccaacacgaa tcttgaactc atctccctga taacttaacc actgaccaca gtacatgccg
     1441 gaaacatgct caaaaggcaa caagaaaacc tcatcttcat caatagcgat cacctcggct
     1501 aaacgttgac tgggttctat cttataaaag gcgccaatac gcgcttttgg taaacgcgcc
     1561 ttaataatcg tcccgccgat attgataatc ttgccttcat atctggtaga cgaatttaat
     1621 gataatgccg gcacagtgct attgagcact ttctgaatac gtgggtattt ttccaataca
     1681 gaatcatgct ctgaaatcat ttaccgttcc taatacttta agttcgatat tattcccaac
     1741 ctcctgaaaa gagagcacgg ggactgacgg gaacgaacct tcaatcattt tcttcacata
     1801 gcgcctgata tctaaagcgg tcagaatgac gccgttcccc gttggattca tgatattttt
     1861 taagaagcca attatctgct cagttcgctc cggtgagata ttcagatacg aacctgatga
     1921 cgtttgtctg atcgactctc gtagctcatt ttcaatatca gagccaataa gccaaacgtt
     1981 cagaagtgta ccgctaacgc tatagcgacc taaaatatgc cgacgcaggg cgatacgaac
     2041 gtattcgcac aagataacca catccttttc ttttgttgac caaaaaataa gcgtctcaaa
     2101 gatagttctc aggtctctaa ttgagacatt ttcctctacg agacgttgta ggatgtcaac
     2161 aattttgctc aaaccaagct ggcgctgcag ctctttcaca agctcgttat attttctctc
     2221 catgatgtcc atcaaataac gcgtttcttg tacgccgatg aactctttgg cattagacaa
     2281 tacaagtttc tttagcaaaa acgttatctt atcatttgtt gttgcataaa catccatacc
     2341 tgtaaggtga gcgagctgtt gattagcctt attcgtttta tatacgatag agttagtcga
     2401 aagcttttcc tggacaaatt cgaatgatat ctctgcatgc ccagcctcaa aatagacagt
     2461 atcatctata agagtatctt tgtatattga ctcttgatag agcaaaaatt ctattgcgct
     2521 atcattattt ttaaccggat taacaataat cttaggcaat ggaattccac tttcctcaaa
     2581 gaaattccaa cgcatgacct caatttgctg tgtaatgtct gaactataaa tattactact
     2641 taagtttaaa atcaatggct cagcgcccgg tgagatgttt tggctattag acaaatcagc
     2701 tcccatagca tccttattat tgccagttcc aaatgttctt ttcttctgta atttccaggc
     2761 tatccccaac aggcaaaccg ccagaaataa gaagaccaat gtaggaaagc ccggaataat
     2821 cgccattacg agcattagtg cggcactgat taaaaaaggg cgactattaa cagaaacctg
     2881 ttgaacaata tcacctgcaa ggttttcttc tttttcgctt tcaccgggca cacgagtaac
     2941 aaccacgccc gcggtgacag aaataataag cgcagggatc tgggcaacca aagcatcacc
     3001 gacagacaat acagaaaaca ggctaagtgc ctcactaaat ggcatgtcaa attgccacat
     3061 accaatgagc acgccaccaa aaaggttaac taaaacaata atgataccag caatagcgtc
     3121 cccttttaca aacttcatcg ccccgtccat tgaaccataa aactggcttt ccttttgcac
     3181 tcgggagcga agaacttttg cctcaagcgg gtcaataacg cctgcgcgca tatcaccatc
     3241 aatactcatt tgctttcctg gcataccatc aagggaaaaa cgagcactca cttcagcaac
     3301 acgctcggcc ccttttgtaa taaccataaa ttgaacaatg gtgataatag taaaaataac
     3361 caggccaaca acaatattac cgccaacaac aaagttaccg aaggagtaaa taatgtcacc
     3421 cgcatcgtga tgcagcagaa taagtctggt tgtactaaca ctgagcgaca ggcgcatcaa
     3481 cgtcgtaatc aacaggattg tggggaaaga agttagttca agaggatttt ttatatagat
     3541 tgagagcatt aataaaagta aagctgtcga aatattaatc gcgataatga tatccaccac
     3601 aactgttggt agtggaataa tcatcatcat tacagccata aagaagaaga gagccagaat
     3661 aagatcgtga tatgactctg cttttttaaa tatatttaag agtttattca tgatgtcatc
     3721 ctgcgaacgc gctcaataat ctgcacatgc tgttgaaaag tattcattag aaaaaaaaca
     3781 tcgatattgg ttaacaaaca acttaagcac aatttattat cgtagacaaa aatatgttca
     3841 atcactccct gcgtacgctc aggagcaagg gcactaaaga a
//
LOCUS       DADSYM010000224         3815 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2415
            SAMN08039579-rid11723173.denovo.226, whole genome shotgun sequence.
ACCESSION   DADSYM010000224 DADSYM010000000
VERSION     DADSYM010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039579
            Sequence Read Archive: SRR6362533
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3815)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3815)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980162.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 03:39:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,484
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 4,347
            CDSs (with protein)               :: 4,347
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 43 of 85
            Pseudo Genes (incomplete)         :: 37 of 85
            Pseudo Genes (internal stop)      :: 19 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3815
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039579-rid11723173.denovo.226"
                     /strain="LGE2415"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8302 N 95.9628 W"
                     /collection_date="2015-08-24"
     gene            <1..321
                     /gene="mdtJ"
                     /locus_tag="J1Y83_004169"
     CDS             <1..321
                     /gene="mdtJ"
                     /locus_tag="J1Y83_004169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug/spermidine efflux SMR transporter
                     subunit MdtJ"
                     /protein_id="HBA7632252.1"
                     /translation="ITGTLSMKWASVSEGNGGFILMLVMISLSYIFLSFAVKKIALGV
                     AYALWEGIGILFITLFSVLLFDESLSLMKIAGLTTLVAGIVLIKSGTRKARKPELEVN
                     HGAV"
     gene            308..637
                     /gene="mdtI"
                     /locus_tag="J1Y83_004170"
     CDS             308..637
                     /gene="mdtI"
                     /locus_tag="J1Y83_004170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug/spermidine efflux SMR transporter
                     subunit MdtI"
                     /protein_id="HBA7632253.1"
                     /translation="MAQFEWVHAAWLALAIVLEIVANVFLKFSDGFRCKIFGLLSLAA
                     VLAAFSALSQAVKGIDLSVAYALWGGFGIAATLAAGWILFGQRLNRKGWIGLVLLLAG
                     MIMVKLA"
     gene            complement(676..1497)
                     /locus_tag="J1Y83_004171"
     CDS             complement(676..1497)
                     /locus_tag="J1Y83_004171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HBA7632254.1"
                     /translation="MRTTIAVVLGAISLTSAFVFADKPDVAKSANDEVSTLFFGHDDR
                     VPVNDTTQSPWDAVGQLETASGNLCTATLIAPNLALTAGHCLLTPPKGKADKAVALRF
                     VSNKGLWRYEIHDIEGRVDPTLGKRLKADGDGWIVPPAAAPWDFGLIVLRNPPSGITP
                     LPLFEGDKAALTAALKAAGRKVTQAGYPEDHLDTLYSHQNCEVTGWAQTSVMSHQCDT
                     LPGDSGSPLMLHTDDGWQLIGVQSSAPAAKDRWRADNRAISVTGFRDKLDQLSQK"
     gene            complement(1597..1680)
                     /locus_tag="J1Y83_004172"
     CDS             complement(1597..1680)
                     /locus_tag="J1Y83_004172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA7632255.1"
                     /translation="MVGRYRFEFILIILILCALIAARFYLS"
     gene            complement(1773..2108)
                     /locus_tag="J1Y83_004173"
     CDS             complement(1773..2108)
                     /locus_tag="J1Y83_004173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310330.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid shock protein"
                     /protein_id="HBA7632256.1"
                     /translation="MKKQIEGMTMKKVLALVVAAAMGLSSAAFAAETATTPAPTATTT
                     KAAPAKTTHHKKQHKAAPAQKAQAAKKHHKNAKAEQKAPEQKAQAAKKHAKKHSHQQP
                     AKPAAQPAA"
     gene            complement(2506..3759)
                     /locus_tag="J1Y83_004174"
     CDS             complement(2506..3759)
                     /locus_tag="J1Y83_004174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBA7632257.1"
                     /translation="MSRTTTVDGAPASDTDKQSISQPNQFIKRGTPQFMRVTLALFSA
                     GLATFALLYCVQPILPVLSQEFGLTPANSSISLSISTAMLAIGLLFTGPLSDAIGRKP
                     VMVTALLLASICTLLSTMMTSWHGILIMRALIGLSLSGVAAVGMTYLSEEIHPSFVAF
                     SMGLYISGNSIGGMSGRLISGVFTDFFNWRIALAAIGCFALASALMFWKILPESRHFR
                     PTSLRPKTLFINFRLHWRDRGLPLLFAEGFLLMGSFVTLFNYIGYRLMLSPWHVSQAV
                     VGLLSLAYLTGTWSSPKAGTMTTRYGRGPVMLFSTGVMLFGLLMTLFSSLWLIFAGML
                     LFSAGFFAAHSVASSWIGPRAKRAKGQASSLYLFSYYLGSSIAGTLGGVFWHNYGWNG
                     VGAFIALMLVIALLVGTRLHHRLHA"
BASE COUNT          979 a          914 c          983 g          939 t
ORIGIN      
        1 attaccggta cgctgtcaat gaaatgggcg agcgtcagtg agggaaatgg cggctttatt
       61 ttaatgctgg tgatgatttc tctgtcgtat atatttctct ctttcgccgt taaaaaaatc
      121 gccttaggcg tggcttatgc gctgtgggaa ggtatcggta ttttatttat taccttgttt
      181 agcgttttgt tattcgacga aagtttatcg ctgatgaaaa ttgccgggtt aaccaccctg
      241 gtcgccggga ttgtattgat caaatcaggt acccgtaaag cgcgtaaacc tgaactggag
      301 gtgaaccatg gcgcagtttg aatgggttca cgccgcctgg ctggcattgg caatcgtgct
      361 ggaaatcgtt gctaacgtct ttttgaaatt ttctgacggc tttcgttgca aaatatttgg
      421 cttgctctcc ctggcggcgg tgctggctgc ctttagtgcg ctttctcagg ccgttaaagg
      481 gatcgacttg tctgtcgctt atgcattgtg gggcgggttt ggtattgccg ccacgttagc
      541 cgcaggttgg atcttgtttg gtcaacggtt aaatcgtaaa ggctggattg gcctggtgct
      601 gctgttggct ggaatgatca tggtgaaact tgcctgatga acacgctgcc cgcgctgtcg
      661 ggcagcgttt gaacattatt tttgcgacag ttgatccagc ttgtcgcgaa aaccggtaac
      721 agaaatggcc cggttatcgg cgcgccagcg atctttcgcg gcaggagccg agctttgcac
      781 cccaattaat tgccagccgt catcggtatg caacatcaga ggcgaaccgc tgtcacccgg
      841 caaggtatcg cactgatgcg acatcaccga cgtttgcgcc cagccagtca cttcacagtt
      901 ttgatgactg tacaacgtat cgagatgatc ttcagggtag cccgcctgag tcactttacg
      961 accagccgct tttaatgcgg cggtaagcgc ggctttatct ccctcaaata acggcaacgg
     1021 tgtaatgcca gaagggggat tacgtagcac aatcaaaccg aagtcccagg gcgcagctgc
     1081 gggaggtaca atccagccat ccccatctgc ttttaaccgc tttcccagtg tcggatcaac
     1141 gcggccttct atgtcgtgga tctcatagcg ccaaagacct ttatttgaca cgaaacgcag
     1201 cgctaccgct ttatcggctt taccctttgg cggcgtcaat aaacagtgtc ctgccgttaa
     1261 tgccagattg ggtgcaatca gtgtcgccgt acataaattg ccgctggccg tttccagttg
     1321 gccaaccgca tcccacggtg attgggtcgt gtcattcact ggcacacgat catcatgacc
     1381 aaaaaacagg gtgctgacct catcgtttgc cgatttggca acgtctggtt tatctgcaaa
     1441 cacaaaagca gacgtcaaac taattgcacc caacactaca gcaatggttg tacgcatatc
     1501 acactctggt gggtaattat gattattaaa agcaaaccct cataaatact atagacggga
     1561 cgggatgaaa gtgggagtaa aatcagataa ctacgatcag gaaagataaa aacgggcggc
     1621 aatcagtgcg cataaaataa ggatgataag aatgaactca aagcgataac ggcccaccat
     1681 actaccctcc gctaaaggcg gtgctaaacg ttacccgctt agcaccgtaa ttttcaggcg
     1741 cgagggggcg cgccagcatt actgttgaaa acttacgctg cgggttgtgc agcaggtttt
     1801 gccggttgct gatggctgtg tttcttggcg tgtttcttcg ccgcctgcgc tttttgttca
     1861 ggggctttct gttcagcttt ggcattttta tgatgctttt tagccgcctg cgctttctgg
     1921 gcaggtgctg ctttatgctg ttttttatga tgtgtggttt tcgcaggcgc tgctttggtg
     1981 gtcgtcgcag tcggagcagg tgtggtcgca gtctctgcag caaaggcagc agaagacaga
     2041 cccatagcag cggcaacaac cagagctaat acttttttca ttgtcatacc ctcaatttgt
     2101 tttttcattt aaccccactg cggggccgtt gaaataacta tatccctggg aatggcagac
     2161 ttccgtgagt ggttggtttc agcgtgtaac gatatgtaca aacgctgaat aaattacagc
     2221 gttgataata acgtattgtg acttaaggaa aatttagcta ccaataatag tagtcttgat
     2281 cgtgttaact attttactac ttacaaagcg gtagaataac tgcgcatcag taataaatga
     2341 cacacagcaa aatgaatccg tttatttggg tacttataat cctgatgacg ctagatgcgc
     2401 tgcgggaatt ggctggcgct tcgtctattt taggatggct attaacgttg gtttgagcag
     2461 gcaataagtc cggacgggta tttacctcag tccggactta ttttttcagg cgtgcagacg
     2521 atgatgcaaa cgcgtcccga ccagcagagc aatgaccagc atcagagcaa taaatgcgcc
     2581 gacgccgttc cagccatagt tatgccagaa aacaccaccc agcgtcccgg caatactcga
     2641 ccccagatag taactgaaca gatacagcga ggaggcctgg cctttagcgc gttttgcgcg
     2701 ggggccgatc cagctgctgg caacggagtg ggctgcgaag aatccggctg agaagagtaa
     2761 cattccggca aagatcagcc acagcgagct gaataaggtc atcagtaaac caaacagcat
     2821 aacccccgtc gaaaacaaca tcaccggacc gcgtccatag cgggtggtca tggttccggc
     2881 tttgggtgag ctccatgtac cggtcaaata agccagcgat aataagccaa ccacggcctg
     2941 actgacatgc cagggggaaa gcatcaacct atagccgatg taattaaaca gcgtgacgaa
     3001 cgaccccatc agcaaaaagc cttctgcgaa caataacggt aatccccggt cacgccagtg
     3061 cagacgaaag ttgatgaata acgtcttagg gcgcagcgaa gtcgggcgaa aatggcgtga
     3121 ttcagggagg attttccaga acatcaacgc cgaggccaac gcgaaacaac cgattgccgc
     3181 cagagcaatt cgccagttaa aaaagtccgt gaagacaccg ctaattaagc gtccgctcat
     3241 gccgccaatt gagttgccgc tgatatacaa ccccattgaa aaggccacga aactggggtg
     3301 gatttcctcg ctaagataag tcatgccaac ggctgccacg ccacttaacg aaagcccaat
     3361 caaggcgcgc ataatcaaaa tgccgtgcca gctggtcatc attgtcgaaa gtaacgtaca
     3421 aatggaggcc aacagtagcg ccgtgaccat cactggtttg cgaccaatgg catcggatag
     3481 cgggccagta aacagcaaac cgatagccaa catcgccgtt gaaatggaca gtgaaatact
     3541 actgttcgcg ggcgttaagc caaactcttg tgaaagcacc ggaaggatag gttgcacaca
     3601 atagagaagt gcgaaagttg ccagtccggc agagaaaagc gccaaggtga cgcgcataaa
     3661 ttgcggcgta ccgcgtttaa taaattgatt tggctgagaa atgctttgct tgtcagtgtc
     3721 gcttgccgga gcgccatcaa cagttgtagt acggctcact tgaaatcctt gctaaatatg
     3781 cctgtggatc aggcttatga agagattagg aaaat
//
LOCUS       DADSYM010000225         3794 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2415
            SAMN08039579-rid11723173.denovo.227, whole genome shotgun sequence.
ACCESSION   DADSYM010000225 DADSYM010000000
VERSION     DADSYM010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039579
            Sequence Read Archive: SRR6362533
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3794)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3794)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980162.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 03:39:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,484
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 4,347
            CDSs (with protein)               :: 4,347
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 43 of 85
            Pseudo Genes (incomplete)         :: 37 of 85
            Pseudo Genes (internal stop)      :: 19 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3794
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039579-rid11723173.denovo.227"
                     /strain="LGE2415"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8302 N 95.9628 W"
                     /collection_date="2015-08-24"
     gene            complement(<1..535)
                     /locus_tag="J1Y83_004175"
     CDS             complement(<1..535)
                     /locus_tag="J1Y83_004175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001555725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HBA7632258.1"
                     /translation="MNLIFSKYSVFFILFVFFAFPLSGQANDSVVVTFTGILLAKSCD
                     ITTGSKDQSVKMGSYDANEFLNVGDVSPSQTFTINVQGCPTAKSTVYSSGVSANVRFT
                     GDADAINSALLRLSAGADSATGLGIEILDNNDVAIAINGESGFRDLVLNENGDANLTF
                     KLRYKSTQENVHAGQANA"
     gene            complement(535..3129)
                     /locus_tag="J1Y83_004176"
                     /pseudo
     CDS             complement(535..3129)
                     /locus_tag="J1Y83_004176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001622451.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis usher protein"
     gene            complement(3166..>3794)
                     /locus_tag="J1Y83_004177"
     CDS             complement(3166..>3794)
                     /locus_tag="J1Y83_004177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000649745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HBA7632259.1"
                     /translation="AGGFGLGVTRLIYPAESKQITLSVQNSGDNTSYLIQSWVDDSKT
                     EKKSQDFVITPPLFMLPTRKEASLRIMFLGKTNLPTDRETLYWMNVKAIPPTDEKNTQ
                     KNTLQLALQNKIKLFYRPENLPVQPGKARNMLRFKYEGKQLKVINPSPYYLTLTGIKV
                     QGSKLPNTFVPPKSDITVLSPVTLAGEITYQTINDFGATTERQKGIMQ"
BASE COUNT         1119 a          856 c          717 g         1102 t
ORIGIN      
        1 gagcatttgc ttgcccggca tgtacatttt cctgagttga tttatagcga agtttaaacg
       61 tcaaattagc atcaccgttt tcatttaaca ctaaatcacg aaaacctgat tcaccattaa
      121 tggcaatcgc cacatcattg ttatccaata tttctattcc cagtccagtc gcactatctg
      181 caccggcgga gagacgcagt aatgcgctat taatggcatc tgcatcacca gtaaaccgca
      241 cattggcgct cacaccagaa ctataaactg tactttttgc agtcgggcat ccttgtacat
      301 taatcgtaaa cgtttgactc ggagaaacat cacctacatt taaaaattca ttagcatcat
      361 aagagcccat ttttaccgac tgatctttac ttccggtagt aatgtcacag ctttttgcca
      421 acaaaatccc agtaaatgta acaacaactg aatcattggc ctgccctgag agcggaaaag
      481 caaaaaaaac gaaaagaata aaaaatacag aatatttaga gaatattaaa ttcattagcg
      541 gcactccaga gttgcatagc taacaggatt atccagttct gtttcaggga gcttgtaatt
      601 agcctgacat ttttgctgaa caccgtctcc ccactggata ttaataacac cttctaaagg
      661 caatcctgag agataaagtt gtccattatc gccgacaatc ccggtaattt gactactatt
      721 gttaatctgt gcgctggctg tagcaccgaa tgggactgat ttattacgat aacgaactgt
      781 gaacaaacca cgaataccga tatgagcagc aaactctgca cgaactaacg caccttttcc
      841 cggaataact tcagtggaag tgatatccag ttcagtatgc atattcattg acataggatc
      901 taatgaaatg ttattacgtc tatattctgt tgcataagga acaactgcat atccacgcca
      961 gtcagtggat attcctgtcc cccgctggac atccacatta ttcgctccag gcgcttttat
     1021 caaaacatta gtttcaccta atggttgact caacgtaaga ccattttcat gtacaacaag
     1081 agcaccactg atgccatagt ttatttgttg attaccttca ttatcgtaac tgtaggcgga
     1141 attcaaggaa ccatatgccc cacgataatt aacaccaaca gaaccactac tgctattatc
     1201 ctgattagca aaaccatgtt gtatgttata actgagatta tgctgctcca acagagaacc
     1261 agataccccc gtatattggt ttacctgtcc gtggttatcc tgactataac catacgttgc
     1321 agtcatggca ttcgatgatg agcgcccatc attcgtggaa gataaccaat tacttatagg
     1381 taatgagaca tttagcgaga caattttatc tgcatttgtc cctggtgatt tactgtaatt
     1441 ccacgcaaca tttagataga tacccttaat actggtgtta taaccaactt ggtataaaga
     1501 atctttttta tcagtattcc agtatgtttg ctgactagcc gataaataca tagacccgta
     1561 gttaccgaaa ggttgcgata cactagcctg aaattttcca cgtttgctat aacgcaagtt
     1621 ataaaaatca ttccagttat aaacgtaatc tttatcatct aacgtttttg gatcaacaac
     1681 agtccctgac atttgttggt atgtcgtatc acttaaagtg taaaagccct aggttgaata
     1741 acgatagcca ataatatgga atgctgttcc tgtctgaact aacgatttgg aataaagaaa
     1801 acgataagat tgcccttgat gtttgctccc atccacaagc gaactgttag cctgagtaac
     1861 atcaaacgat gttgcaccaa atacacccag attcaggcca aggccgagcg ccaacgcccg
     1921 gtaatcctca gagaattgtg ccccaccata agccgtaata tcccagggta aaccccaaat
     1981 taattccccc tgaaatatat aaggactttt ctggttatag ctattgcttc gatattggcc
     2041 aaccgtagta gaatattttg aatgaccttc tcgttgcagg atggggacag cggcataagg
     2101 tacggtatag acagtttctg aattatcaga ctctttgatg gtaacttcca gatctcccgc
     2161 agatgatgtc gggttaagat cgctaatctc aaacggtccc ggcggcatat aggtttgata
     2221 aatgacataa ccattctgtt tgattgttac ctgagcgcga cttttcgcaa ttccgcgcac
     2281 cgtgggagcg aaaccacttt gactatcggg caacatattt tcgtcagatt ccagttttat
     2341 gccacgaata ctaacactgt cgaaaacatc tgatgatgac catgtatctc ctagcgttaa
     2401 ttcacttctg aaaggaataa taacgcgctg caacgtacta ctgatatgct ccagtttacc
     2461 tgctgaatta ctcccacgac tccaggttga ataatcgcga aaacgccatg caccaatatt
     2521 gatcccagcg cgaagattta aataatagtt atttgaagag taatcaccac gatcgttatt
     2581 accagaaaag tcataattca gcaaaaaagc tgtaataccg ttatcccact gttctggtgg
     2641 aatatatccg cgggcgtcac gttttattat cgcctgcgga aaggtcagtt taagtctctg
     2701 tgcatccagc tccggcatgt aatgcacatc aggaaaccag atacgcaaat caaggcaact
     2761 attttcagat gaagccaaag cctgctctgg cagcgcattt ttgttaattc caaggcttaa
     2821 taagtcagca agtgaaaggc agggaatcag ttgattctca tcgtctgctt taaaagttac
     2881 agtacgagag agtaaagggg aatcattgac ccaaacttcg acttcatagc taccaggaag
     2941 ataagtcatc cctttctcga agcgggataa gtcagcaaca gcttcagtat cacccgacag
     3001 aaaagcaggg ttaaagcgca ttcccgcgat tgaacatgca ggcataataa ccagaagaag
     3061 cggcacaacc cgtttcatct gcttttgtgc cgccatccaa tattcgcatc ttttgagctt
     3121 taggttcata tccctgacct gtgttgcatc tgaactattc cgttgttact gcattattcc
     3181 tttctgccgc tccgtcgtag cgccaaagtc attaattgtt tgataagtaa tttcaccagc
     3241 caacgtcact ggcgacaaga ccgtaatatc acttttcggt ggtacaaacg tattgggtaa
     3301 tttactccct tgtactttaa ttcctgtcag tgtcaggtaa taaggcgaag gattgataac
     3361 ttttagttgt ttgccctcat acttaaaccg caacatattt cgtgcttttc ctggttgcac
     3421 aggcagattt tccgggcgat aaaatagctt gattttgttt tgtaatgcca actgtaaggt
     3481 attcttctgt gtatttttct catctgttgg cggaattgct ttaacattca tccaatacaa
     3541 agtttcacga tcggtcggca aattagtctt accaagaaac atgatacgta atgaagcttc
     3601 tttacgcgtt ggcaacataa agaggggagg agtgataaca aaatcctggc tctttttttc
     3661 cgttttactg tcatcaaccc atgattgaat taaataactg gtgttgtcac cgctattttg
     3721 cacactgagt gttatttgct tactttctgc cggataaatc aatctcgtta cgccgaggcc
     3781 aaacccgccc gcgg
//
LOCUS       DADSYM010000226         3617 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2415
            SAMN08039579-rid11723173.denovo.228, whole genome shotgun sequence.
ACCESSION   DADSYM010000226 DADSYM010000000
VERSION     DADSYM010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039579
            Sequence Read Archive: SRR6362533
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3617)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3617)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980162.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 03:39:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,484
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 4,347
            CDSs (with protein)               :: 4,347
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 43 of 85
            Pseudo Genes (incomplete)         :: 37 of 85
            Pseudo Genes (internal stop)      :: 19 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3617
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039579-rid11723173.denovo.228"
                     /strain="LGE2415"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8302 N 95.9628 W"
                     /collection_date="2015-08-24"
     gene            <1..2805
                     /gene="eae"
                     /locus_tag="J1Y83_004178"
     CDS             <1..2805
                     /gene="eae"
                     /locus_tag="J1Y83_004178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intimin"
                     /protein_id="HBA7632260.1"
                     /translation="CYSRTRHKHKLKKTFIMLSAGLGLFFYVNQNSFANGENYFKLGS
                     DSKLLTHNSYQNRLFYTLKTGETVADLSKSQDINLSTIWSLNKHLYSSESEMMKAAPG
                     QQIILPLKKLPFEYSALPLLGSAPLVAAGGVAGHTNKLTKMSPDVTKSNMTDDKALNY
                     AAQQAASLGSQLQSRSLNGDYAKDTALGIAGNQASSQLQAWLQHYGTAEVNLQSGNNF
                     DGSSLDFLLPFYDSEKMLAFGQVGARYIDSRFTANLGAGQRFFLPENMLGYNVFIDQD
                     FSGDNTRLGIGGEYWRDYFKSSVNGYFRMSGWHESYNKKDYDERPANGFDIRFNGYLP
                     SYPALGARLMYEQYYGDNVALFNSDKLQSNPGAATVGVNYTPIPLVTMGIDYRHGTGN
                     ENDLLYSMQFRYQFDKPWSQQIEPQYVNELRTLSGSRYDLVQRNNNIILEYKKQDILS
                     LNIPHDINGTERSTQKIQLIVKSKYGLDRIVWDDSSLRSQGGQIQHSGSQSAQDYQAI
                     LPAYVQGGSNIYKVTARAYDRNGNSSNNVQLTITVLSNGQVVDQVGVTDFTADKTSAK
                     ADGTEAITYTATVKKNGVAQVNVPVSFNIVSGTAVLSANSANTNGSGKATVTLKSDKP
                     GQVVVSAKTAEMTSALNANAVIFVDQTKASITEIKADKTTAKANGSDAVTYTVKVMKG
                     GTPVSGQKVTFSKDFGTLDKTEATTDQNGYATVKLSSSTPGKAIVSAKVSDVDTEVKA
                     TTVEFFTPLSIDGNKVTVIGTGVTGSLPNNWLQYGKVKLQAAGGNGKYTWKSSDTKIA
                     SVDSTGVITLNEKGSATITVVSGDNQSATYTINAPSSIVIAVDKNTRVTYSEAENKCQ
                     TNGAALAQSKEILANIYSSWGAANKYPYYSSSKSLTTWIKQSSSELSSGVSSTYDLVT
                     KNQLTNVGVKNKNAFAVCVK"
     gene            complement(3009..>3617)
                     /gene="escD"
                     /locus_tag="J1Y83_004179"
     CDS             complement(3009..>3617)
                     /gene="escD"
                     /locus_tag="J1Y83_004179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE inner membrane
                     ring protein EscD"
                     /protein_id="HBA7632261.1"
                     /translation="NNIICNNQIISAINDVLTEYGYKDIIITKDNKPGFFLLSGYIPP
                     SPKWSEVENLLLNTPGVAGWEIHNNSNNKINELASEFKKNKLINYVNIFKKNDVIIVA
                     GEVSQQNESKILAIINAMNKNSNVKILFQNIQPYISADIFPGKILRISGTMKNPTIAL
                     DNGTSLGIGSILKGGYVIDAIDPKDGINISRPDEYIHIPLSY"
BASE COUNT         1047 a          674 c          809 g         1087 t
ORIGIN      
        1 tgttattccc ggacccggca caagcataag ctaaaaaaaa catttattat gcttagtgct
       61 ggtttaggat tgttttttta tgttaatcag aactcatttg caaatggtga aaattatttt
      121 aaattgggtt cggattcaaa actgttaact cataatagct atcagaatcg ccttttttat
      181 acgttgaaaa caggtgaaac tgttgccgat ctttctaaat cgcaagatat taatttatcg
      241 acgatttggt cgttgaataa gcatttatac agttctgaaa gcgaaatgat gaaggccgcg
      301 cctggtcagc agatcatttt gccactcaaa aaacttccct ttgaatacag tgccttacca
      361 cttttaggtt cggcacctct tgttgctgca ggtggtgtcg ctggtcatac aaataaactg
      421 actaaaatgt ccccggacgt gaccaaaagc aacatgaccg atgacaaggc attaaattat
      481 gcggcacaac aggcggcgag tctcggtagc cagcttcagt cgcgatctct gaacggcgat
      541 tacgcgaaag ataccgctct tggtatcgct ggtaaccagg cttcgtcaca gttgcaggcc
      601 tggttacaac attatggaac ggcagaggtt aatctgcaga gtggtaataa ctttgacggt
      661 agttcactgg acttcttatt accgttctat gattccgaaa aaatgctggc atttggtcag
      721 gtcggagcgc gttacattga ctcccgcttt acggcaaatt taggtgcggg tcagcgtttt
      781 ttccttcctg aaaacatgtt gggctataac gtcttcattg atcaggattt ttctggtgat
      841 aatacccgtt taggtattgg tggcgaatac tggcgagact atttcaaaag tagtgttaac
      901 ggctatttcc gcatgagcgg ctggcatgag tcatacaata agaaagacta tgatgagcgc
      961 ccagcaaatg gcttcgatat ccgttttaat ggctatctgc catcataccc ggcattaggc
     1021 gccaggctga tgtatgagca gtattatggt gataatgttg ctttgtttaa ttctgataag
     1081 ctgcagtcga atcctggtgc ggcgaccgtt ggtgtaaact atactccgat tcctctggtg
     1141 acgatgggga tcgattaccg tcatggtacg ggtaatgaaa atgatctcct ttactcaatg
     1201 cagttccgtt atcagtttga taaaccgtgg tctcagcaaa ttgagccaca gtatgttaac
     1261 gagttaagaa cattatcagg cagccgttac gatctggttc agcgtaataa caatattatt
     1321 ctggagtaca aaaagcagga tattctttct ctgaatattc cgcatgatat taatggtact
     1381 gaacgcagta cgcagaagat tcaattgatc gttaagagca aatacggtct ggatcgtatc
     1441 gtctgggatg atagttcatt acgtagccag ggcggccaga ttcagcatag cggaagccaa
     1501 agcgcacaag attaccaggc tattttgcct gcttatgtgc aaggtggtag caacatttat
     1561 aaagtgacgg ctcgtgccta tgaccgtaat ggcaatagct ctaacaatgt acagcttact
     1621 attaccgttc tgtcgaatgg tcaggtggtc gaccaggttg gggtaacgga ctttacggcg
     1681 gataagactt cggctaaagc ggatggcacc gaagcaatta cttatactgc gacggtgaaa
     1741 aagaatgggg tagctcaggt taatgtcccg gtttcattta atattgtttc aggaactgca
     1801 gttttaagtg ccaatagtgc caataccaat ggtagcggta aggcgactgt aaccctgaaa
     1861 tcggataaac caggccaggt cgtcgtgtct gctaaaacag cagagatgac ttcagcgctt
     1921 aatgccaatg cagttatatt tgttgatcaa accaaggcca gcattactga gattaaggct
     1981 gataaaacaa ctgcgaaggc aaatggttcc gatgctgtta catacactgt taaagtgatg
     2041 aaggggggaa cccctgtgtc aggtcaaaaa gtgaccttct ctaaggattt tgggacactg
     2101 gataagactg aagcaacaac tgatcagaat ggttatgcta ctgtaaaact gtcatctagc
     2161 actccaggca aagctattgt tagcgcaaaa gtgagtgatg tagatacaga agttaaggct
     2221 actactgttg agttttttac cccactaagt atcgatggta ataaagtgac tgtaattggt
     2281 actggtgtca ctggttctct gccaaataac tggctgcagt atgggaaggt taagctacag
     2341 gctgcgggag gcaatgggaa atatacatgg aaatccagtg atactaaaat cgcctctgtt
     2401 gatagtacgg gtgtgataac cttaaatgag aaagggagtg ccacaattac tgtagtatct
     2461 ggagataatc agagtgcgac atatacaatt aatgcgccaa gcagtatcgt aattgctgtg
     2521 gataaaaata ctcgagttac gtattctgag gctgaaaata aatgccagac aaatggagca
     2581 gctttagcac agtcaaaaga aatattggca aatatttatt cttcgtgggg ggctgcgaat
     2641 aaatatcctt attattctag ctctaaatca ttgactactt ggattaaaca atcctcttct
     2701 gaactctcat caggtgtatc aagtacatat gatttagtta cgaagaacca attgactaat
     2761 gtgggagtaa aaaataaaaa tgcttttgca gtttgtgtaa aataacttag ttgcatcgca
     2821 aaacccggga aataaatttt attttccggg ttttttatat ctctgcttgg actttaaacg
     2881 aatgattcgt gttaaaagca atgtcttatt tctgttataa gcttttttct atgttgagtt
     2941 gatgactgtc ttgctcataa catccacaca ataaaaaacc ctccgaagag ggggaagagg
     3001 ataagaaatt aatacgacag tggaatatgt atatattcgt ctgggcggga gatgttgata
     3061 ccatctttag gatcaattgc gtctatgaca tagcctcctt tcagaatgct accaatcccc
     3121 aggctggtac cattatcaag agcaattgtt ggattcttca tcgtaccgct gattctgagt
     3181 atttttccgg gaaaaatatc tgctgaaatg tacggctgaa tgttctgaaa gagtatcttg
     3241 acgttactat ttttgttcat tgcatttata atggcaagga tcttactctc gttctgttga
     3301 gaaacttccc ccgcaacaat gatgacatca ttctttttga aaatattgac atagtttatt
     3361 aatttattct ttttaaactc tgatgccagt tcattgattt tattgttact gttgttgtgt
     3421 atttcccatc ctgcaactcc tggcgtattc agtaacagat tttcaacctc gctccatttg
     3481 ggtgaagggg gaatgtatcc cgataacaga aagaatccag gcttattatc ttttgtaata
     3541 atgatatctt tgtaaccgta ttccgttaag acatcattaa ttgcggatat gatttggtta
     3601 ttgcaaatga tattatt
//
LOCUS       DADSYM010000227         3507 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2415
            SAMN08039579-rid11723173.denovo.229, whole genome shotgun sequence.
ACCESSION   DADSYM010000227 DADSYM010000000
VERSION     DADSYM010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039579
            Sequence Read Archive: SRR6362533
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3507)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3507)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980162.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 03:39:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,484
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 4,347
            CDSs (with protein)               :: 4,347
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 43 of 85
            Pseudo Genes (incomplete)         :: 37 of 85
            Pseudo Genes (internal stop)      :: 19 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3507
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039579-rid11723173.denovo.229"
                     /strain="LGE2415"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8302 N 95.9628 W"
                     /collection_date="2015-08-24"
     gene            complement(170..862)
                     /locus_tag="J1Y83_004180"
     CDS             complement(170..862)
                     /locus_tag="J1Y83_004180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000712403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBA7632262.1"
                     /translation="MKKETLADRLNLAMEQSGMSQGALAKASGVAQPTIWRLTSGNAR
                     GSTKIVEIANALGVRTEWLSSGIGPMRNDGQQSGKPAVSHSKYFKIDVLDIEVSAGPG
                     VINREFVEVLRSVEYSFDDARHMFDGRKAENIRIINVRGDSMSGTIEPGDLLFVDITV
                     KSFDGDGIYAFLYDDTAHVKRLQMMKDKLLVISDNKSYSPWDPIEKDEMNRVFIFGKV
                     IGSMPQTYRKHG"
     gene            973..1200
                     /locus_tag="J1Y83_004181"
     CDS             973..1200
                     /locus_tag="J1Y83_004181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001580007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBA7632263.1"
                     /translation="MTNQTIQLAISITGSQKRLADLCGVAQPTVWRWLHGGGIDARYV
                     MKIVSATGGKIKPADIRPDLAPLFNASNSAA"
     gene            1231..1770
                     /locus_tag="J1Y83_004182"
     CDS             1231..1770
                     /locus_tag="J1Y83_004182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001182892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="regulator"
                     /protein_id="HBA7632264.1"
                     /translation="MQPLTYQQTSGFSPTAVINRSQTKQAPGHEKIRDAVRAWSAADN
                     QDVVAALIVNEYREQGGGTIDFPDDVSRARQKLFRFLDNKFDSEKYRNNVRELTPAIL
                     AVLPLEYRGHLVEQDSFMARLAEMEKELSEAKQAVILNAPRHQKLKEMSEGIVSMFRV
                     DPDLAGPLMAMVTTMLGAI"
     gene            1857..2786
                     /locus_tag="J1Y83_004183"
     CDS             1857..2786
                     /locus_tag="J1Y83_004183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016247981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HBA7632265.1"
                     /translation="MANTAEIFNFPVPDAAQKEPRVADLDDGYTRIANELLEAVMLAG
                     LTQHQLLVFLAVMRKTYGFNKKLDWVSNEQLSELTGILPHKCSAAKSVLVKRGILIQS
                     GRNIGINNVVSEWSTLPESGKKNKVYLKEVNLPESGKKSLPKSGKGTYPNQVNTKDKL
                     TKDNIKPFSSENSDESSDQPENALPVVKPDAAIQSGSKWGTAEDLTAAEWMFDMVKTI
                     APSARKPNFAGWANDIRLMRERDGRNHRDMCVLFRWACQDNFWSGNVLSPAKLRDKWT
                     QLEINRNKQQAGVTAGKPKLDLTNTDWIYGVEL"
     gene            2783..3493
                     /locus_tag="J1Y83_004184"
     CDS             2783..3493
                     /locus_tag="J1Y83_004184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000788780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Replication protein P"
                     /protein_id="HBA7632266.1"
                     /translation="MKNIAAQMVNFDREQMRRIANNMPEQYDEKPQVQQVAQIINGVF
                     SQLLATFPASLANRDQNELNEIRRQWVLAFRENGITTMEQVNAGMRVARRQNRPFLPS
                     PGQFVAWCREEASVNAGLPNVSELVDMVYEYCRKRGLYPDAESYPWKSNAHYWLVTNL
                     YQNMRANALTDAELRRMAADELSCMTARINRGEAIPEPVKQLPVMGGRPLNRVQSLEK
                     IKQLRAKHGLCRGKRFSG"
BASE COUNT          966 a          832 c          868 g          841 t
ORIGIN      
        1 attactgctt ttttataatg aacgactttt tgtctgttat ctttcattgc tatagtccaa
       61 ctagtattga ataaataaac acagtaaaga cataaaaatt tcttatagct accgataacg
      121 taatcggtag ctattacaaa tcaaccaaag gtaattcttt acatactacc tacccatgct
      181 tcctgtaggt ctgcggcatg ctcccaataa ctttcccgaa gataaacacc cggttcatct
      241 cgtctttctc gataggatcc cacggtgagt agctcttgtt atcagagata accagcagtt
      301 tatccttcat catttgcagg cgctttacat gggcggtgtc gtcgtacaga aatgcataga
      361 taccatcacc gtcgaaagat ttaaccgtga tatcaacgaa cagcagatca cctggttcga
      421 tcgttcctga catgctgtca ccacgcacgt taatgatgcg gatattttct gccttcctgc
      481 catcgaacat gtgacgagca tcgtcaaacg agtactcaac cgagcgtaga acttctacaa
      541 actcacggtt gatgactccc ggcccggcac tgacttctat atcaagaacg tcaattttga
      601 agtatttgga atggctgaca gcaggcttcc ctgattgttg accgtcattt ctcatcgggc
      661 ctatgcctga tgagagccat tctgttcgaa cacccaatgc attagctatt tcaacaattt
      721 ttgttgagcc gcgcgcgttg ccgcttgtca gtctccagat tgtgggttga gctacgccag
      781 acgcctttgc aagagcgcct tgagacattc cagattgttc catcgctagg tttaagcgat
      841 cagcaagagt ttcttttttc ataagtttta atttatacgc ttgcgtattg atggtcaaaa
      901 cacgttttgc tattgcttgg attaatacgc attgctatta ttcattcatt gcaataccaa
      961 taggaattga taatgacaaa tcaaaccatt caactcgcaa tcagtattac aggtagtcaa
     1021 aagcgactgg cagatctatg cggtgtagcc cagcccactg tttggcgttg gctacacggt
     1081 ggcggaattg atgcccgcta tgtaatgaaa attgtctcag ccactggtgg aaagattaaa
     1141 ccagcagata ttcgtcccga cctcgcacca ttgtttaacg cgagtaattc tgccgcctaa
     1201 tctgcggcgt taactgataa ggcaatgact atgcaaccac ttacatacca acagactagc
     1261 ggatttagcc cgactgcggt gataaatcgt tctcaaacaa aacaagctcc aggccacgaa
     1321 aaaatccgtg atgccgtccg cgcctggtcg gctgcagata atcaggatgt tgttgccgca
     1381 ctcattgtga atgagtatcg ggagcagggc ggcggcacca tcgatttccc tgatgatgtc
     1441 agccgtgcac gccagaagct gttccgcttc ctcgataaca aattcgattc tgaaaaatac
     1501 cgaaataacg tgcgtgaact gaccccggca attctggcgg tactaccgct ggaatatcgc
     1561 ggccacctgg ttgagcagga tagcttcatg gctcggctgg ctgaaatgga aaaggaactc
     1621 agtgaggcaa aacaggctgt cattctcaac gcaccacgcc accagaaact gaaggagatg
     1681 agtgaaggca ttgtgtcgat gtttcgtgtg gacccggatc tggctggtcc actgatggcg
     1741 atggtcacca ccatgctggg ggcaatatga caggttcgga aatggcgaaa gccggtctgc
     1801 gcgaacagaa ccgactttca ggtgcaaatc gtaacacact cattgcggga ggaattatgg
     1861 caaacactgc tgagatattc aattttccag tgccggatgc ggcacaaaag gagccgcgcg
     1921 tggcagatct cgatgatggt tatacgcgca ttgcaaatga gttgctggaa gctgtgatgc
     1981 tggccggatt aacacagcac cagcttctgg tctttctggc tgtcatgcgc aaaacatatg
     2041 gctttaataa aaaactggat tgggttagca acgagcaact ttccgaatta accgggatat
     2101 tgccgcacaa gtgttctgct gcaaaaagtg ttctggtaaa gcgtgggatt cttattcaga
     2161 gcgggcggaa tatcggcatc aataatgtgg tcagtgaatg gtcaacatta cccgaatcag
     2221 gtaagaaaaa taaagtttac ctgaaagagg taaatttacc tgaatcaggt aagaaaagtt
     2281 tacccaaatc aggtaaaggc acttacccga atcaggtaaa cacaaaagac aaactaacaa
     2341 aagacaatat aaaacctttt tcgtccgaga attctgacga atcctctgac cagccagaaa
     2401 atgcccttcc tgtggtgaaa ccggatgctg cgattcagag cggcagcaag tgggggacag
     2461 cagaagacct gactgccgca gagtggatgt ttgacatggt gaagaccatc gcgccatcag
     2521 ccagaaaacc gaattttgca gggtgggcta acgatatccg cctgatgcgt gaacgtgacg
     2581 gacgtaacca ccgcgacatg tgcgtgctgt tccgctgggc ctgccaggac aacttctggt
     2641 ccggtaacgt gctgagtccg gccaaactac gcgacaagtg gacccagctc gaaatcaacc
     2701 gtaacaagca acaggctggc gtgacagccg gaaaaccaaa actcgacctg acgaacactg
     2761 actggattta cggggtggag ctatgaaaaa catcgccgca cagatggtta attttgaccg
     2821 tgagcagatg cgccggatcg ccaataacat gccggaacag tacgacgaaa agccgcaggt
     2881 acagcaggta gcgcagatta tcaatggtgt gttcagccag ttactggcaa ctttcccggc
     2941 gagcctggct aaccgggacc agaatgaact gaacgaaatc cgccgccagt gggttctggc
     3001 tttccgggaa aatgggatca ccacgatgga acaggttaac gctggaatgc gcgtagcccg
     3061 tcggcagaat cgaccattcc tgccatcacc cgggcagttt gtcgcctggt gccgggaaga
     3121 agcatccgtt aacgccgggc tgccaaacgt cagcgagctg gttgatatgg tctatgagta
     3181 ttgtcggaag cgtggcctgt atccagatgc agagtcttat ccatggaaat caaacgcgca
     3241 ctactggctg gttaccaacc tgtaccagaa catgcgggcc aatgcgttga ctgacgcgga
     3301 attacggcgc atggctgccg atgaactgtc ctgtatgacc gcgcgaatta accgtggtga
     3361 agcgatccct gaaccggtga aacaacttcc tgtcatgggc ggcaggccgc ttaatcgtgt
     3421 tcagagcttg gaaaaaatta aacaattgcg tgctaaacat ggattatgta gagggaaaag
     3481 gtttagtgga tgaaagtaat gaggtga
//
LOCUS       DADSYM010000228         3456 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2415
            SAMN08039579-rid11723173.denovo.230, whole genome shotgun sequence.
ACCESSION   DADSYM010000228 DADSYM010000000
VERSION     DADSYM010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039579
            Sequence Read Archive: SRR6362533
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3456)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3456)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980162.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 03:39:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,484
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 4,347
            CDSs (with protein)               :: 4,347
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 43 of 85
            Pseudo Genes (incomplete)         :: 37 of 85
            Pseudo Genes (internal stop)      :: 19 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3456
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039579-rid11723173.denovo.230"
                     /strain="LGE2415"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8302 N 95.9628 W"
                     /collection_date="2015-08-24"
     gene            63..896
                     /gene="xapA"
                     /locus_tag="J1Y83_004185"
     CDS             63..896
                     /gene="xapA"
                     /locus_tag="J1Y83_004185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthosine phosphorylase"
                     /protein_id="HBA7632267.1"
                     /translation="MYQAQFSHNPLYCVDIIKTYKPDFTPRVAFILGSGLGALADQIE
                     NAVAISYEKLPGFPVSTVHGHAGELVLGYLQGVPVACMKGRGHFYEGRGMTIMTDAIR
                     TFKLLGCELLFCTNAAGSLRPEVGAGSLVALKDHINTMPGTPMVGLNDERFGERFFSL
                     ANAYDAEYRALLQKVAKEEGFPLTEGVFVSYPGPNFETAAEIRMMQIIGGDVVGMSVV
                     PEVISARHCELKVVAVSAITNMAEGLSDVKLSHAQTLAAAELSKQNFINLICGFLRKI
                     A"
     gene            957..2213
                     /gene="xapB"
                     /locus_tag="J1Y83_004186"
     CDS             957..2213
                     /gene="xapB"
                     /locus_tag="J1Y83_004186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthosine/proton symporter XapB"
                     /protein_id="HBA7632268.1"
                     /translation="MSIALRLKVMSFLQYFIWGSWLVTLGSYMINTLHFTGANVGMVY
                     SSKGIAAIIMPGIMGIIADKWLRAERAYMLCHLVCAGVLFYAASVTDPDMMFWVMLVN
                     AMAFMPTIALSNSVSYSCLAQAGLDPVTAFPPIRVFGTVGFIVAMWAVSLLHLELSSL
                     QLYIASGASLLLSAYALTLPKIPVAEKKATTSLASKLGLDAFVLFKNPRMAIFFLFAM
                     MLGAVLQITNVFGNPFLHDFARNPEFADSFVVKYPSILLSVSQMAEVGFILTIPFFLK
                     RFGIKTVMLMSMVAWTLRFGFFAYGDPSPTGFILLLLSMIVYGCAFDFFNISGSVFVE
                     QEVDSSIRASAQGLFMTMVNGVGAWVGSILSGMAVDYFSVDGVKDWHTIWLMFAGYAL
                     FLAVIFFFGFKYNHDPEKITHRSVTH"
     gene            2266..2424
                     /locus_tag="J1Y83_004187"
     CDS             2266..2424
                     /locus_tag="J1Y83_004187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001392449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1427 family protein"
                     /protein_id="HBA7632269.1"
                     /translation="MKDILFSSGVGFGIGALFTIVRLPIPVPNVLPGILSIVFMYVGY
                     LVVKYFMP"
     gene            2465..3349
                     /gene="xapR"
                     /locus_tag="J1Y83_004188"
     CDS             2465..3349
                     /gene="xapR"
                     /locus_tag="J1Y83_004188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator XapR"
                     /protein_id="HBA7632270.1"
                     /translation="MERVYRTDLKLLRYFLAVAEELHFGRAAARLNMSQPPLSIHIKE
                     LENQLGTQLFIRHSRSVVLTHAGKILMEESRRLLVNANNVLARVEQIGRGEAGRIELG
                     VVGTAMWGRMRPVMRRFLRENPNVEVLFREKMPAMQMALLERRELDAGIWRMATEPPA
                     GFTSLRLHESAFLVAMPEEHHLSSFSTVPLEALRDEYFVTMPPVYTDWDFLQRVCQQV
                     GFSPVVIREVNEPQTVLAMVSMGIGITLIADSYAQMNWPGVIFRPLKERIPADLYIVY
                     ETQQVTPALVKLLAALAQ"
BASE COUNT          763 a          752 c          942 g          999 t
ORIGIN      
        1 tgaggtaggg caatctcgtt ataaaaaggc gttagactcc accctacaaa aaaaggatat
       61 atatgtatca ggctcagttt tctcataacc cactgtattg cgtagatatt atcaagactt
      121 ataaacctga tttcacgcca cgagtggcct ttattttagg ttccgggctg ggcgcgctgg
      181 ccgatcagat tgagaacgcg gtcgcaattt cctacgaaaa gctgcctggg ttcccggtaa
      241 gtaccgtaca cggtcatgcg ggtgagctgg tgctggggta tctccagggg gtgccagtgg
      301 cgtgcatgaa aggtcgcgga catttctacg aaggtcgtgg gatgaccatc atgacggatg
      361 caatccgtac ctttaagttg ctgggctgcg agttgctgtt ctgcaccaat gcggctggct
      421 cactgcgtcc tgaagtgggg gccggcagtc tggtcgcatt gaaagatcac atcaacacca
      481 tgccgggaac gccgatggtg ggtcttaatg atgaacgttt tggtgagcgc ttcttctcgc
      541 tggcgaatgc ctacgatgcg gaataccgcg cactgttaca aaaagtggcg aaagaagagg
      601 ggttccctct gacggagggc gtgttcgtct cgtatccggg gccgaatttc gagactgcgg
      661 cggaaattcg catgatgcaa attattggtg gggatgttgt tggtatgtct gtggtgcctg
      721 aggttatttc agctcgccat tgcgaactta aagtcgttgc ggtctctgcg attaccaaca
      781 tggcggaagg tctgagtgac gtgaagcttt ctcatgccca aacgctggca gcagcggaac
      841 tctcaaagca aaactttatt aatcttattt gcggctttct gcgcaaaatt gcctgagtaa
      901 aacaacaata aacggcctta ctgagtgagg ccatatgatg cgacaaggaa acgattatga
      961 gcatcgcttt gcgcttaaag gtaatgtcct ttttgcaata ttttatctgg gggagctggc
     1021 tggttaccct cggttcttac atgattaata cccttcattt caccggcgct aatgttggca
     1081 tggtttacag ttccaaaggg atcgccgcga ttattatgcc gggtataatg gggatcatcg
     1141 cagacaaatg gctgcgtgca gaacgtgcat acatgctgtg tcacctggtg tgtgcgggcg
     1201 tactttttta tgcggcatcc gtaactgatc cggatatgat gttttgggtg atgttagtca
     1261 atgcgatggc gtttatgccg actattgcgt tatcgaacag cgtctcttat tcctgtcttg
     1321 cccaggcagg gcttgacccg gtgaccgctt tcccgcccat tcgcgttttt ggtacggtgg
     1381 ggttcattgt cgccatgtgg gcagtaagct tgctgcatct ggaattgagt agtctgcaac
     1441 tgtatatcgc gtccggtgcg tcattgctgc tgtcggctta tgcgctgact ttgccgaaga
     1501 ttccggttgc ggagaaaaaa gcgactacat cgcttgccag caagctgggt ctggatgcct
     1561 tcgtgctgtt taaaaatcca cgcatggcta tctttttcct cttcgccatg atgttgggcg
     1621 cggtgctgca aattaccaac gtttttggca acccgttcct gcatgatttc gcccgtaatc
     1681 ctgagtttgc tgatagcttc gtggtgaaat acccgtccat tttactgtca gtttcacaga
     1741 tggctgaagt cggtttcatt ctgacgatac cgttcttttt gaagcgtttc ggtattaaaa
     1801 ccgtcatgct gatgagtatg gtggcgtgga cgctgcgctt tggcttcttt gcctatggcg
     1861 atccatcacc aaccgggttt attttgctgt tgctgtcgat gattgtttac ggttgtgcat
     1921 tcgatttctt caatatttct ggttcggtct ttgtcgaaca ggaagttgat tccagcattc
     1981 gtgccagcgc ccagggcctt tttatgacga tggtaaatgg cgtcggcgca tgggttggct
     2041 cgattctgag cggtatggca gtggattact tctcggtgga tggcgtaaaa gattggcata
     2101 ccatttggct gatgtttgca gggtatgctc ttttcctcgc agtgatattt ttctttgggt
     2161 ttaaatataa ccatgaccct gaaaagataa cgcatcgttc agttacacat taaaaggatt
     2221 cgcagctctg ttccttgggg ctgcttttat gataaaggtt aattagtgaa agatatttta
     2281 ttttcttctg gcgttggttt tggcattggt gctttgttta caatcgtgcg cttgcctatt
     2341 cctgtaccaa atgtattacc gggtatatta tccattgtgt ttatgtatgt cggatatctg
     2401 gtggtgaaat actttatgcc atgataattt aatacgatgt atttattata tggagcactt
     2461 aattatggaa cgcgtataca gaacagatct taagttgctc cgttattttc ttgccgtagc
     2521 ggaagagttg cattttggtc gcgcagcagc gcgtttaaat atgtctcagc ctccgctcag
     2581 cattcatatt aaagagctgg aaaatcaact cggcacgcag ctttttattc gccattcgcg
     2641 cagcgtcgta ctgacacacg cgggcaaaat cttgatggaa gaatcgcgtc gattgctggt
     2701 gaatgcaaat aatgtattgg ctcgggttga acaaataggt cggggagaag cagggcggat
     2761 tgaactcggg gttgtgggaa cggcaatgtg gggacggatg cgcccggtta tgcggcgatt
     2821 ccttagagaa aatcctaacg ttgaagttct gtttcgcgaa aagatgcccg cgatgcaaat
     2881 ggccctgctg gaacgccgcg aacttgatgc cgggatctgg cgaatggcga cagaaccacc
     2941 ggcaggtttt accagcttac ggttgcatga atcggcgttt ctggtggcga tgcctgaaga
     3001 gcatcatctc tcatcatttt ccaccgtccc gctggaagcg ctacgtgacg agtatttcgt
     3061 tacaatgccg cccgtgtaca ctgactggga ttttttgcag cgggtttgcc agcaggtggg
     3121 attttcaccg gttgttattc gcgaagttaa tgaaccgcaa acggtactcg ccatggtcag
     3181 tatgggtatt ggtatcacat tgatagcgga cagctacgcg cagatgaact ggcccggtgt
     3241 catattccgt ccgctcaaag aacgcatccc ggcagactta tatattgttt atgaaacgca
     3301 gcaggtgacg cccgcgctgg ttaaactgct ggcggcgttg gcgcagtaga tgagaggcac
     3361 gggaactgcc ggacatagtt ggtctgacaa gcgaagcgca tcaggcaatt tcgcaaattg
     3421 cagatagcaa aaaagcgcct ttagggcgct ttttta
//
LOCUS       DADSYM010000229         3404 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2415
            SAMN08039579-rid11723173.denovo.231, whole genome shotgun sequence.
ACCESSION   DADSYM010000229 DADSYM010000000
VERSION     DADSYM010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039579
            Sequence Read Archive: SRR6362533
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3404)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3404)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980162.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 03:39:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,484
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 4,347
            CDSs (with protein)               :: 4,347
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 43 of 85
            Pseudo Genes (incomplete)         :: 37 of 85
            Pseudo Genes (internal stop)      :: 19 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3404
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039579-rid11723173.denovo.231"
                     /strain="LGE2415"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8302 N 95.9628 W"
                     /collection_date="2015-08-24"
     gene            complement(96..422)
                     /locus_tag="J1Y83_004189"
     CDS             complement(96..422)
                     /locus_tag="J1Y83_004189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbL family protein"
                     /protein_id="HBA7632271.1"
                     /translation="MKRTLLLCAFLVGLVSSNVMALTLDEARTQGRVGETFYGYLVAL
                     KTDAETEKLVTDINAERKASYQQLAKQNNVSVDDIAKLAGQKLVERAKPGEYVQGING
                     KWMRKF"
     gene            complement(430..615)
                     /locus_tag="J1Y83_004190"
     CDS             complement(430..615)
                     /locus_tag="J1Y83_004190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnbE family lipoprotein"
                     /protein_id="HBA7632272.1"
                     /translation="MKILLAALTSSFMLVGCTPRIEVAAPKEPITINMNVKIEHEIII
                     KADKDVEELLETRSDLF"
     gene            complement(612..3251)
                     /locus_tag="J1Y83_004191"
     CDS             complement(612..3251)
                     /locus_tag="J1Y83_004191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbH family protein"
                     /protein_id="HBA7632273.1"
                     /translation="MLGKYKAVLALLLLIILVPLTLLMTLGLWVPTLAGIWLPLGTRI
                     ALDESPRITRKGLIIPDLRYLVGDCQLAHITNASLSHPSRWLLSVGTVELDSACLAKL
                     PQTEQSPAAPKTLAQWQSMLPNTWINIDKLIFSPWQEWQGKLSLALTSDIQQLRYQGE
                     KVKFQGQLKGQQLTVSELDVAAFENQPPVKLVGEFTMPLVPDGLPVSGHATATLNLPQ
                     EPSLVDAELDWQENSGQLIVLARDNGDPLLDLPWQITRQQLTVSDGRWSWSYAGFPLS
                     GRLGVKVDNWQAGLENALVSGRLSVLTQGQAGKGNAVLNFGPGKLSMDNSQLPLQLTG
                     EAKQADLILYARLPAQLSGSLSDPTLTFESGALLRSKGRVIDSLDIDEIRWPLAGVKV
                     TQRGVDGRLQAILQAHENELGDFVLHMDGLANDFLPDAGRWQWRYWGKGSFTPMNATW
                     DVAGKGEWHDSTITLTDLSTGFDQLQYGTMTVVKPRLILDKPVVWGRDAQHPSFSGAL
                     SLDAGQTLFTGGSVLPPSTLKFSVDGRDPTYFLFKGDLHAGEIGPVRVNGRWDGIRLR
                     GNAWWPKQSLTVFQPLVPPDWKMNLRDGELYAQVAFSAAPEQGFRAGGHGVLKGGSAW
                     MPDNQVNGVDFVLPFRFADGAWHLGTRGPVTLRIAEVINLVTAKNITADLQGRYPWTE
                     EEPLLLTDVSVDVLGGNVLMKQLRMPQHDPALLRLNNLSSSELVSAVNPKQFAMSGAF
                     SGALPLWLNNEKWIVKDGWLANSGPMTLRLDKDTADAVVKDNMTAGSAINWLRYMEIS
                     RSSTRINLNNLGLLTMQANITGTSRVDGKSGTVNLNYHHEENIFTLWRSLRFGDNLQA
                     WLEQNARLPGNDCPQGKECEDKQ"
BASE COUNT          837 a          927 c          763 g          877 t
ORIGIN      
        1 ataattcagc cttataatta aaagagcaaa atgtttccgg aaaatagatt aatcgccttc
       61 tcctgtagcg ttatcccgat attgcggttt tgctattaaa attttcgcat ccatttaccg
      121 tttatccctt gtacatattc cccgggtttg gcccgttcta cgagcttttg cccggcgagt
      181 ttcgcgatat catccaccga cacattattt tgctttgcca gttgttgata gctcgcttta
      241 cgttcggcat tgatgtcagt tactaatttc tcagtttcag catctgtttt cagcgcaacc
      301 agataaccat aaaatgtttc ccctacccgc ccctgagttc tggcttcatc cagagtcaat
      361 gccattacat tgctgcttac caagccaaca agaaatgcac aaagaagtaa tgttcttttc
      421 atcatcacct caaaaaagat cgctacgggt ctcaagcagc tcttcgacat ctttgtctgc
      481 cttgatgatg atctcatgct caattttaac gttcatattg atagtgatcg gttccttagg
      541 tgcagcgact tcaatgcgag gcgtacagcc aaccagcata aaagatgacg tcaacgcagc
      601 cagtaaaatt ttcattgttt atcctcacac tcttttcctt gcggacagtc atttcccggc
      661 agacgcgcgt tctgctccag ccatgcctgg agattatcgc caaagcgtaa actgcgccac
      721 agcgtaaaaa tattctcttc atgatggtaa ttaagattta ccgtaccgct tttaccatca
      781 acgcgactgg tacctgtaat gttggcctgc atggttaata aaccgagatt atttaaatta
      841 attcttgtcg atgaacggct aatttccata tagcgcaacc agttaattgc tgaacccgcg
      901 gtcatattgt ctttcaccac cgcatctgcg gtgtctttat ccagccgcaa ggtcatcggc
      961 ccgctattag ccagccagcc gtctttcact atccattttt cgttgtttag ccataacggc
     1021 aatgcaccac taaatgcccc ggacatggcg aattgtttcg gattgacggc gctaaccagt
     1081 tcgctggagg aaagattatt cagccgcaac agcgccgggt catgttgcgg catacgtaat
     1141 tgtttcatca gcacgttacc gcctaacaca tcgacgctaa catcagtcaa cagcaatggt
     1201 tcttcttcag tccacggata acgaccttgc aaatcagccg taatattttt cgctgtcacc
     1261 agattaatca cttcggcaat tcgcaacgta acgggaccgc gagtccccag atgccaggct
     1321 ccatcggcaa aacggaaagg caggacaaaa tcgacaccgt taacctgatt atctggcatc
     1381 caggcactac cgcctttcaa cacgccgtgc cctcccgcgc ggaatccttg ttcgggcgct
     1441 gctgaaaatg cgacctgagc atacagttca ccatcgcgta agttcatctt ccagtcgggt
     1501 ggcaccaacg gctggaatac agtcagtgat tgtttaggcc accaggcgtt accgcgcaga
     1561 cgaataccgt cccagcgacc atttacccga accgggccaa tctcaccagc atgtaaatcg
     1621 cctttaaaga ggaaatacgt cggatcgcgc ccatcgacac taaattttaa ggttgatggc
     1681 ggtaacacac tgccgccagt gaacagcgtt tgcccggcgt ccagtgacag agcgccgctg
     1741 aaggagggat gctgtgcgtc acgtccccag acgacgggct tgtcgagaat taatctcggc
     1801 tttactaccg tcatcgtacc gtattgtaac tggtcgaaac cggtggagag atcggtcagc
     1861 gtaatcgtgc tgtcatgcca ctcacctttt cctgcgacat cccaggtggc attcatcggt
     1921 gtaaaactcc cttttcccca gtagcgccac tgccagcggc cagcgtcagg gagaaaatca
     1981 ttcgccagcc catccatatg cagcacgaaa tcgcccagtt cattttcatg cgcctgcaaa
     2041 atggcctgca aacgtccgtc aacaccacgt tgggtgactt ttacacccgc taaaggccag
     2101 cggatttcat cgatgtccag cgaatcgatg actcttccct tcgaacgtaa taacgcgcct
     2161 gactcaaagg tcagcgttgg gtcagacaga cttccgctta gctgcgcagg taaacgggca
     2221 tataaaatga ggtccgcctg tttcgcttca ccggtcagct gcagaggcag ctggctgtta
     2281 tccatgctta attttcctgg gccaaaattt aacaccgcgt tgcctttacc cgcttgcccc
     2341 tgggtcagca cactcagtcg cccgctgacc agagcgttct cgagccctgc ctgccagttg
     2401 tcgactttga cacctagtcg gccactcaaa ggaaaacctg catacgacca gctccagcga
     2461 ccatcgctta cggtcaattg ttgacgagta atttgccacg gcaaatcgag caacggatcg
     2521 ccgttatccc gtgccagcac aatcaattgc ccgctatttt cctgccagtc cagctcggca
     2581 tccaccagtg acggttcctg cggcaagttt aacgtcgcag tagcatgacc acttaccgga
     2641 agtccatccg gcacgagcgg catagtaaat tcccccacca gttttaccgg cggctgattt
     2701 tcaaacgcgg cgacatccag ttcgctgact gtaagttgtt gccctttcag ctggccttga
     2761 aatttaactt tttcgccctg ataacgcagt tgctggatat cggaggttaa tgcgagagag
     2821 agttttccct gccattcctg ccagggagaa aaaatcagtt tatcgatatt gatccaggtg
     2881 ttaggcagca tggactgcca ctgcgcgagg gtttttggag cggctggcga ttgctccgtc
     2941 tgcggcaatt tcgccagaca agcagaatca agttctaccg tgccgacgct caataaccag
     3001 cggctgggat gtgaaaggct ggcgttggtg atatgcgcaa gctgacaatc tcccaccaga
     3061 taacggagat cggggatgat taaaccttta cgcgtaatgc gtgggctttc atctaatgca
     3121 atacgtgtcc cgagcggtag ccagatgccc gccagcgtgg gaacccacag cccgagcgtc
     3181 atcagcagcg tcaacggcac aagaataatc agtaataaca gcgcgagaac ggctttatat
     3241 ttacccagca tgggtagtta atatcctgat ttagcgaaaa attaagcatt caatacgggt
     3301 attgtggcat gtttaaccgt tcagttgaag gttgcgccta cactaagcat agttgttgat
     3361 gaatttttca atatcgccat atctttcaat tatatttgaa attt
//
LOCUS       DADSYM010000230         3386 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2415
            SAMN08039579-rid11723173.denovo.232, whole genome shotgun sequence.
ACCESSION   DADSYM010000230 DADSYM010000000
VERSION     DADSYM010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039579
            Sequence Read Archive: SRR6362533
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3386)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3386)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980162.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 03:39:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,484
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 4,347
            CDSs (with protein)               :: 4,347
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 43 of 85
            Pseudo Genes (incomplete)         :: 37 of 85
            Pseudo Genes (internal stop)      :: 19 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3386
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039579-rid11723173.denovo.232"
                     /strain="LGE2415"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8302 N 95.9628 W"
                     /collection_date="2015-08-24"
     gene            complement(<1..90)
                     /locus_tag="J1Y83_004192"
     CDS             complement(<1..90)
                     /locus_tag="J1Y83_004192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001062775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HBA7632274.1"
                     /translation="MAKILPSIFGADILRLKDEIHFLEEEQTEI"
     gene            complement(113..1366)
                     /locus_tag="J1Y83_004193"
     CDS             complement(113..1366)
                     /locus_tag="J1Y83_004193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008924526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HBA7632275.1"
                     /translation="MEMLKNLMETITGMGATAILPLVIFILGLVFRMKPGAAIKSGIT
                     VGIGFIGLGLVVGLLNSSLQPAIEYYSKVGSGFTVADIGWPAVGAAAWVAPFAALVIP
                     VGIVLNLILVRLKLTKTLNVDIWNYMHFLVPGALAYFVFDSFIIGFSVAVALSIAALF
                     IGDLIAPRWQKYYGLEGTTCTTMIHIGWTLPFAWVVNKIIDYIPGLNKLDVDLNSVQK
                     RLGVFGEPAIIGIIVGALLGVLTKQAITTIVPMAMGVAGVMVLLPKVVGVLMEGLNPI
                     GKSAKEIMQKQMGKDAELNIGMDCALALGDPATVTVTVITIPLTMLCALVLPDIKIFP
                     IGVLMSIIYMTTMTVMVSNGNVVRSIISTLLFCVVVMYLGGYVAPGATQFLAGAGVGL
                     QGQGTDFVLTGPWEILTYWLSTVLH"
     gene            complement(1387..1680)
                     /locus_tag="J1Y83_004194"
     CDS             complement(1387..1680)
                     /locus_tag="J1Y83_004194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004105500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBA7632276.1"
                     /translation="MINILVACGSGVATSTLAADEVKSVCAEYGITAFKINKCSMAEL
                     SSEMAHADIVLTTNNYKGDIGKPHMSVAGFVTGINEGALRKKLGELLTELANG"
     gene            complement(1699..2142)
                     /locus_tag="J1Y83_004195"
     CDS             complement(1699..2142)
                     /locus_tag="J1Y83_004195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001764648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIA"
                     /protein_id="HBA7632277.1"
                     /translation="MISISVADGAGLQNYDAVLEHIFNTHRTTNTVKESWLSAMRERE
                     AEYPTGIELEGYSIAIPHCDSEHANQPAIYIIRLPDAIPVNRADEDGILWVRLVVNLI
                     VTDPADQLRLLKSLFNHLQNEEFYQNLLTLPVEDAKALFVSTIIQ"
     gene            complement(2481..3236)
                     /locus_tag="J1Y83_004196"
     CDS             complement(2481..3236)
                     /locus_tag="J1Y83_004196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000281586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HBA7632278.1"
                     /translation="MYLVDERRKLILEDLMSAGTVYVSDLARKYEVTYETIRKDLTHL
                     EQKGLLIKSHGGATLKQRAIEHPFQVREKENNSYKKAIARKALDFIPPNSSMIIGTGS
                     TTFELANLLCMKSGFKIFTDSLPVASVLIESDNQVFLFGGELRRKSSSVFGGWAVSMI
                     NDIHVDLCFIGTDGFSNMTGPSTPSSSDAFLDRTIISHSDKKYILGDYTKFERSSLFK
                     ICDWSEITALITNDHADKEGIAEVAKYTSVITC"
BASE COUNT         1031 a          791 c          637 g          927 t
ORIGIN      
        1 tatttcggtt tgttcctctt ccagaaaatg aatttcatct ttcaggcgta aaatatccgc
       61 gccaaatata gaaggtaata tttttgccat gttaaaactc cagatcatta aatcaatgta
      121 atacggtact caaccaatag gttaaaattt cccacgggcc ggttaataca aaatcagtac
      181 cttgtccttg caagcctaca ccggctccag ctaaaaattg cgttgcccct ggtgcgacat
      241 aaccgcctaa atacatcact acaacgcaga ataacaaggt cgagataatc gaacgaacca
      301 cgttaccatt gctgaccatt acggtcatgg tggtcatata aataattgac atcaatacgc
      361 caattgggaa gatcttaata tcaggtaata ccagagcaca tagcatggtt aaaggaatgg
      421 taatgactgt cacggtgacc gtcgccggat cccccaatgc cagtgcacaa tccataccga
      481 tgtttaattc agcatcttta cccatctgtt tttgcatgat ttctttggca cttttcccaa
      541 tcgggttaag accttccatc agcacaccga ccactttagg taacaatacc attacaccag
      601 caacccccat cgccatggga acaattgtgg taatggcctg tttcgttaaa actccgagta
      661 atgcgccaac gataataccg ataattgcag gttcaccaaa gacacccagg cgtttttgca
      721 cgctgtttaa gtcaacatct aacttattta acccggggat gtaatcaatg attttattta
      781 ctacccaggc gaacggtaga gtccagccga tatgaatcat cgtggtacag gtcgttccct
      841 cgagtccata atatttttgc catctgggcg caatcaaatc gccaataaac agagccgcaa
      901 tactcaaagc caccgcgaca gagaaaccga tgataaaact gtcgaacaca aaatatgcca
      961 gagcacccgg aactaaaaaa tgcatatagt tccagatatc aacatttaac gttttggtta
     1021 atttcagacg caccagaatc aggttaagta cgatgccaac tggtatcact aatgcggcaa
     1081 aaggtgccac ccatgcagca gcaccgacgg ctggccaacc aatatctgca accgtaaagc
     1141 cactgcctac ttttgagtaa tactcaatag ctggctgtaa tgaactgttt aataaaccaa
     1201 caaccagacc taaaccaata aaaccaatac caacagtgat gcccgacttg atcgcggcac
     1261 cgggtttcat tctgaacacc agacctaaaa taaatatcac cagcggcagt attgcagtcg
     1321 cccccattcc ggtgattgtt tccattaaat ttttcaacat ttccattttt attattcctg
     1381 tcaggtttag ccatttgcta attcagttaa caactcccct aatttcttgc gtaatgctcc
     1441 ttcattaata cccgttacaa aacctgcaac gctcatatga ggttttccaa tatcaccttt
     1501 atagttatta gttgtgagta caatatctgc atgagccatc tcagaagaaa gttcagccat
     1561 actgcattta ttgattttaa atgccgtaat accatattca gcacatactg acttcacttc
     1621 atcagctgca agcgtagatg tagcgacacc gctaccacaa gcgacaagaa tattaatcat
     1681 aataaactcc agtttttgct attggattat tgtggataca aaaagtgctt ttgcatcctc
     1741 gacgggaagt gtcagtaagt tttggtagaa ttcttcattt tggagatggt taaataaaga
     1801 ctttaacaat cgaagctggt cagcaggatc cgtaacaata agattaacga cgagtctgac
     1861 ccacaatatt ccatcttcat cggccctgtt cacaggtatt gcgtctggca gacggatgat
     1921 ataaatagcc ggttgatttg catgttcact atcacagtgt ggtatagcga tcgaatagcc
     1981 ttccagttct attcctgtcg gatattccgc ttcgcgttca cgcatagcac tgagccagct
     2041 ctctttcacc gtattcgttg tacggtgcgt gttaaaaata tgctcaagca cggcatcgta
     2101 gttctgtaac cccgcaccat cggcaacgct gatcgatatc atattcttgc actctcattc
     2161 gctattgctc atatgagcaa ttcttgattt tcatatgagc agttataaca caacgatcct
     2221 gcggataaac agagagatct gagaagttat acttagatca caaaaaacac ataatggcat
     2281 aattacttat gctcatatga gcaattcatc atgtgcgaaa tgattacttc gccaaaaagg
     2341 aagtaaaact ggcaatatgt agaggatgta ttcaatagag ggcgctaagc aagcatcgcc
     2401 atggatgctc acttgataat tgggaaatga tcacatttgg caatgctgat tcgaagggtc
     2461 aaggcgtgtc gtagtgagtt ctaacaggtt attactgacg tgtattttgc gacttcggca
     2521 atcccctctt tgtcggcatg atcgttggta ataagggcgg tgatctccga ccagtcgcag
     2581 atcttaaaca aactgctgcg ctcaaacttg gtgtaatcac cgaggatata ttttttgtct
     2641 gagtgcgaaa ttatcgtacg atccaaaaac gcatcagaag aagaaggcgt cgatggacca
     2701 gtcatgttgc taaagccgtc ggttccaata aaacagagat caacatgtat atcgttgatc
     2761 atagataccg cccagccccc aaacactgag gaactctttc tacgcagttc gccgccaaag
     2821 agaaaaacct ggttgtcaga ttcaatcaac acactggcaa cggggagcga atctgtgaag
     2881 attttaaacc cagatttcat acataacagg ttagccagct caaaagtggt actgcccgta
     2941 ccgataatca tcgaactatt aggcggaatg aaatcaagcg ctttacgagc aatcgccttc
     3001 ttataagagt tattttcttt ttccctgacc tgaaagggat gctctatggc cctttgcttt
     3061 aaagtcgccc caccatggct tttgatcagc agcccttttt gttcaagatg ggtcaaatct
     3121 ttcctgatgg tctcatacgt tacttcatac tttcgtgcaa gatcactgac atagaccgta
     3181 cctgccgaca ttaagtcttc aagtattaac tttcgccttt catcaaccag atacatcctt
     3241 cctcctgaaa tctattgccc atttgagaaa acgccaagca tagcacagat gatgcaaaca
     3301 aaaacaaaca ctaaccgtgg aaagtacaaa taagcccaat aaagacaatg tttggcaatt
     3361 catatatacg attgatattt aatgta
//
LOCUS       DADSYM010000231         3372 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2415
            SAMN08039579-rid11723173.denovo.233, whole genome shotgun sequence.
ACCESSION   DADSYM010000231 DADSYM010000000
VERSION     DADSYM010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039579
            Sequence Read Archive: SRR6362533
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3372)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3372)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980162.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 03:39:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,484
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 4,347
            CDSs (with protein)               :: 4,347
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 43 of 85
            Pseudo Genes (incomplete)         :: 37 of 85
            Pseudo Genes (internal stop)      :: 19 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3372
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039579-rid11723173.denovo.233"
                     /strain="LGE2415"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8302 N 95.9628 W"
                     /collection_date="2015-08-24"
     gene            213..1145
                     /gene="rfaD"
                     /locus_tag="J1Y83_004197"
     CDS             213..1145
                     /gene="rfaD"
                     /locus_tag="J1Y83_004197"
                     /EC_number="5.1.3.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-glyceromanno-heptose 6-epimerase"
                     /protein_id="HBA7632279.1"
                     /translation="MIIVTGGAGFIGSNIVKALNDKGITDILVVDNLKDGTKFVNLVD
                     LDIADYMDKEDFLIQIMAGEEFGDVEAIFHEGACSSTTEWDGKYMMDNNYQYSKELLH
                     YCLEREIPFLYASSAATYGGRTSDFIESREYEKPLNVYGYSKFLFDEYVRQILPEANS
                     QIVGFRYFNVYGPREGHKGSMASVAFHLNTQLNNGESPKLFEGSENFKRDFVYVGDVA
                     DVNLWFLENGVSGIFNLGTGRAESFQAVADATLAYHKKGQIEYIPFPDKLKGRYQAFT
                     QADLTNLRAAGYDKPFKTVAEGVTEYMAWLNRDA"
     gene            1155..2201
                     /gene="rfaF"
                     /locus_tag="J1Y83_004198"
     CDS             1155..2201
                     /gene="rfaF"
                     /locus_tag="J1Y83_004198"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-heptose--LPS heptosyltransferase RfaF"
                     /protein_id="HBA7632280.1"
                     /translation="MKILVIGPSWVGDMMMSQSLYRTLQARYPQAIIDVMAPAWCRPL
                     LSRMPEVNEAIPMPLGHGALEIGERRKLGHSLREKRYDRAYVLPNSFKSALVPFFAGI
                     PHRTGWRGEMRYGLLNDVRVLDKEAWPLMVERYVALAYDKGIMRTAQDLPQPLLWPQL
                     QVSEGEKSYTCNQFSLSSERPMIGFCPGAEFGPAKRWPHYHYAELAKQLIDEGYQVVL
                     FGSAKDHEAGNEILAALNTEQQAWCRNLAGETQLDQAVILIAACKAIVTNDSGLMHVA
                     AALNRPLVALYGPSSPDFTPPLSHKARVIRLITGYHKVRKGDAAEGYHQSLIDITPQR
                     VLEELNALLLQEEA"
     gene            2205..3197
                     /gene="rfaC"
                     /locus_tag="J1Y83_004199"
     CDS             2205..3197
                     /gene="rfaC"
                     /locus_tag="J1Y83_004199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase RfaC"
                     /protein_id="HBA7632281.1"
                     /translation="MRVLIVKTSSMGDVLHTLPALTDAQQAIPGIKFDWVVEEGFAQI
                     PSWHAAVERVIPVAIRRWRKAWFSAPIKAERKAFREALQAKNYDAVIDAQGLVKSAAL
                     VTRLAHGVKHGMDWQTAREPLASLFYNRKHHIAKQQHAVERTRELFAKSLGYSKPQTQ
                     GDYAIAQHFLTNLPTDAGEYAVFLHATTRDDKHWPEEHWRELIGLLADSGIRIKLPWG
                     APHEEERAKRLAEGFAYVEVLPKMSLEGVARVLAGAKFVVSVDTGLSHLTAALDRPNI
                     TVYGPTDPGLIGGYGKNQVECRSTSMSLADLPAQTVFQNLNLEIIINKLTSEIR"
     gene            3194..>3372
                     /locus_tag="J1Y83_004200"
     CDS             3194..>3372
                     /locus_tag="J1Y83_004200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase RfaL"
                     /protein_id="HBA7632282.1"
                     /translation="MTSTLFFSFEKKNWIAYWNRALVFLFITTYFLGGITRYKHLIVI
                     LMTITTIVYLCKRPK"
BASE COUNT          868 a          813 c          873 g          818 t
ORIGIN      
        1 tttatcatac atcggaatat tgatactaaa gcactattca cgtgcaaatc caacatccgc
       61 catgaaggac tggctaaaac ccaaactagt ttgttgcaat tagcatcctt gcatctctat
      121 gtaaagggct gaagggattc ggatgtgatg gtatgattac agacattcgt gtctgagatt
      181 gtctctgact ccataattca aaggttacag ttatgatcat cgttaccggc ggcgcgggct
      241 ttatcggcag caacatcgtt aaagccctga atgacaaagg catcaccgat attctggtgg
      301 tggataacct gaaagacggc accaagtttg tgaacctggt ggatctggat atcgcggact
      361 atatggataa ggaagacttc ctgatccaga ttatggctgg cgaagagttc ggcgatgtcg
      421 aggcgatttt ccacgaaggc gcgtgctctt ccaccaccga gtgggacggc aagtatatga
      481 tggataacaa ctatcaatac tccaaagagc tgctgcacta ctgtctggag cgcgaaatcc
      541 cgttcctgta tgcctcttcc gcagccactt acggtggacg cacctccgac tttattgaat
      601 cccgcgaata tgaaaaaccg ttgaatgtct acggttactc aaaattcctg tttgatgaat
      661 atgttcgcca aatcctgcct gaagcgaact cgcagattgt tggcttccgt tatttcaacg
      721 tttatggacc gcgtgaaggc cataaaggca gtatggcgag cgtcgctttc catctcaaca
      781 cccagcttaa taacggtgaa tcaccgaagc tgttcgaagg tagcgagaac tttaaacgtg
      841 acttcgtcta tgtgggcgac gtggcagatg taaacctgtg gttcctggaa aatggcgttt
      901 ccggcatctt caacctcggc accggtcgtg cggaatcctt ccaggcagta gcagatgcta
      961 cgcttgctta tcacaagaaa ggccaaatcg aatacattcc gttcccggat aaactgaaag
     1021 gccgctacca ggcgttcact caggcagatc tgacgaatct gcgcgcggcg ggttacgata
     1081 aaccgttcaa aaccgttgcc gaaggcgtaa cggaatacat ggcctggctg aatcgtgacg
     1141 cataagagct ctgcatgaaa atactggtga tcggcccgtc ttgggttggc gacatgatga
     1201 tgtcgcaaag tctctatcgc acgctccaag cgcgctatcc ccaggcgata atcgacgtga
     1261 tggctccggc atggtgccgt ccattattat cgcggatgcc ggaagttaac gaagcgatcc
     1321 ctatgcctct cggtcacgga gcgctggaaa tcggcgaacg ccgcaaactg ggtcatagtc
     1381 tgcgtgaaaa gcgctacgac cgcgcctacg tcttacctaa ctccttcaaa tccgcattag
     1441 tgcctttctt cgcaggtatt cctcatcgca ccggctggcg cggcgagatg cgctacggtt
     1501 tactcaacga tgtacgcgtg ctcgataaag aagcctggcc gctaatggtg gaacgctatg
     1561 tcgcgctggc ctatgacaaa ggtattatgc gtaccgcaca agatctgccg cagccattgt
     1621 tatggccgca gttgcaggtg agcgaaggtg aaaaatcata tacctgtaat caattttcgc
     1681 tttcatcaga acgtccgatg attggttttt gcccgggtgc ggagtttggt ccggcaaaac
     1741 gctggccaca ctaccactat gcggagctgg caaagcagct gattgatgaa ggttatcagg
     1801 tggttctgtt tggctcggcg aaagatcatg aggcgggcaa tgagattctt gccgctttga
     1861 atacagagca gcaggcatgg tgtcggaatc tggcgggaga aacacagctt gatcaagcgg
     1921 ttatcctgat tgcagcctgt aaagccattg ttactaacga ttctggtctg atgcacgttg
     1981 cggcggcgct caatcgtccg ctggttgccc tgtatggacc aagcagcccg gacttcacac
     2041 caccgctatc ccataaagca cgcgtgatcc gcctgattac cggctatcac aaagtgcgta
     2101 aaggtgacgc tgcggagggt tatcaccaga gcttgatcga cattactccc cagcgcgtac
     2161 tggaagaact caacgcgcta ttgttacaag aggaagcctg acggatgcgg gttctgatcg
     2221 ttaaaacatc gtcgatgggc gatgttctcc acacgctgcc cgcactcact gatgcccagc
     2281 aggcaatccc agggattaag tttgactggg tggtggaaga agggttcgca cagattcctt
     2341 cctggcacgc tgccgttgag cgagttattc ctgtggcaat acgtcgctgg cgtaaagcct
     2401 ggttctcggc tcccataaaa gctgaacgca aagcgtttcg tgaagcgcta caagcaaaga
     2461 actatgacgc agttatcgac gcccaggggc tggtaaaaag cgcggcgctg gtgacgcgtc
     2521 tggcgcatgg cgtaaagcat ggaatggact ggcaaaccgc tcgcgaaccg ttagccagcc
     2581 tgttttacaa tcgtaaacat catattgcaa aacagcagca cgccgtagaa cgcacccgcg
     2641 aactgtttgc caaaagtctg ggctatagca aaccacaaac ccagggcgat tatgctatcg
     2701 cacagcattt cctgacgaac ctgcctacag atgctggcga atatgccgta tttcttcatg
     2761 caacaacccg agatgataaa cactggccgg aagaacactg gcgagaattg attggtttac
     2821 tggctgattc aggaatacgg attaaacttc cgtggggcgc gccgcatgag gaagaacggg
     2881 cgaaacgact ggcggaagga tttgcttatg ttgaagtatt gccgaagatg agtctggaag
     2941 gcgttgcccg cgtactggcc ggggctaaat ttgtagtgtc ggtggatacg gggttaagcc
     3001 atttaacggc ggcactggat agacccaata tcacggttta tggacctacc gatccgggat
     3061 taattggtgg gtatgggaag aatcaagtag agtgtcgttc tacaagtatg tctcttgcag
     3121 atttgccagc tcaaaccgtt tttcaaaatt taaatcttga aataataatc aataagttga
     3181 catcggagat aagatgacct caacattatt tttctctttc gagaaaaaaa actggatagc
     3241 gtactggaac agagctcttg tattcttatt cattaccacc tattttttgg gtgggattac
     3301 aaggtataaa catcttattg ttattcttat gacaataacg acaatcgtct atctctgcaa
     3361 acggccaaaa ca
//
LOCUS       DADSYM010000232         3337 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2415
            SAMN08039579-rid11723173.denovo.234, whole genome shotgun sequence.
ACCESSION   DADSYM010000232 DADSYM010000000
VERSION     DADSYM010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039579
            Sequence Read Archive: SRR6362533
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3337)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3337)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980162.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 03:39:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,484
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 4,347
            CDSs (with protein)               :: 4,347
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 43 of 85
            Pseudo Genes (incomplete)         :: 37 of 85
            Pseudo Genes (internal stop)      :: 19 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3337
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039579-rid11723173.denovo.234"
                     /strain="LGE2415"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8302 N 95.9628 W"
                     /collection_date="2015-08-24"
     gene            199..1719
                     /locus_tag="J1Y83_004201"
     CDS             199..1719
                     /locus_tag="J1Y83_004201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312727.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HBA7632283.1"
                     /translation="MSLSIVHTRAALGVNAPPITVEVHISKGLPGLTMVGLPETTVKE
                     ARDRVRSAIINSGYEYPAKKITINLAPADLPKEGGRYDLPIAIALLAASEQLTANKLD
                     EYELVGELALTGALRGVPGAISSATEAIKSGRKIIVAKDNEDEVGLINGEGCLIADHL
                     QAVCAFLEGKHALEHPKPTNAVSRALQHDLSDVVGQEQGKRGLEITAAGGHNLLLIGP
                     PGTGKTMLASRINGLLPDLSNEEALESAAILSLVNAESVQKQWRQRPFRSPHHSASLT
                     AMVGGGAIPGPGEISLAHNGVLFLDELPEFERRTLDALREPIESGQIHLSRTRAKITY
                     PARFQLVAAMNPSPTGHYQGNHNRCTPEQTLRYLNRLSGPFLDRFDLSLEIPLPPPGI
                     LSKTVVPGENSTTVKQRVMAARERQFKRQNKLNAWLDNPEIRQFCKLESEDAQWLEET
                     LIHLGLSIRAWQRLLKVARTIADIDQSDIITRQHLQEAVSYRAIDRLLIHLQKLLT"
     gene            complement(1744..2082)
                     /gene="maoP"
                     /locus_tag="J1Y83_004202"
     CDS             complement(1744..2082)
                     /gene="maoP"
                     /locus_tag="J1Y83_004202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrodomain Ori organization protein MaoP"
                     /protein_id="HBA7632284.1"
                     /translation="MAESFTTTNRYFDNKHYPRGFSRHGDFTIKEAQLLERHGYAFNE
                     LDLGKREPVTEEEKLFVAVCRGEREPVTEAERVWSKYMTRIKRPKRFHTLSGGKPQVE
                     GAEDYTDSDD"
     gene            2201..3040
                     /gene="hdfR"
                     /locus_tag="J1Y83_004203"
     CDS             2201..3040
                     /gene="hdfR"
                     /locus_tag="J1Y83_004203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator HdfR"
                     /protein_id="HBA7632285.1"
                     /translation="MDTELLKTFLEVSRTRHFGRAAESLYLTQSAVSFRIRQLENQLG
                     VNLFTRHRNNIRLTAAGEKLLPYAETLMSTWQAARKEVAHTSRHNEFSIGASASLWEC
                     MLNQWLGRLYQNQDVHTGLQFEARIAQRQSLVKQLHERQLDLLITTEAPKMDEFCSQL
                     LGYFTLALYTSAPSKLKGDLNYLRLEWGPDFQQHEAGLIGADEVPILTTSSAELAQQQ
                     IAMLNGCTWLPVSWARKKGGLHTVVDSTTLSRPLYAIWLQNSDKNALIRDLLKINVLD
                     EVY"
     gene            complement(3136..3211)
                     /locus_tag="J1Y83_004204"
     tRNA            complement(3136..3211)
                     /locus_tag="J1Y83_004204"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3176..3178),aa:Trp,seq:cca)
     gene            complement(3220..3296)
                     /locus_tag="J1Y83_004205"
     tRNA            complement(3220..3296)
                     /locus_tag="J1Y83_004205"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3260..3262),aa:Asp,seq:gtc)
BASE COUNT          896 a          821 c          809 g          811 t
ORIGIN      
        1 aattcaattt cagtatgaat gttgttccct tcctcgtagt tcatcctttg cacgctgcat
       61 ttttgcgagc attattccat cgccagaaca actcaacttc acttgcacaa ataaacagga
      121 atcagccact aaaattcacg aattagcggg ctacagcagc gatatcttat gcacataatg
      181 gtgactcaag gagggcttat gtcactgtca attgttcata cccgcgcagc cctgggagta
      241 aatgcgcccc caatcactgt tgaggtacat atcagtaaag gtctaccagg cttaacgatg
      301 gtgggcttac cagaaacaac ggtaaaagaa gctcgcgatc gcgtgcgcag cgccattatc
      361 aatagcggat atgaatatcc ggcgaaaaaa atcaccatca accttgctcc agccgatctg
      421 ccaaaagaag ggggacgata tgatttgcct atcgctattg cgttactggc ggcctcagaa
      481 cagcttacag ccaataagtt agatgaatat gaattagtcg gagaactggc gcttacaggc
      541 gctctgcgtg gcgttcccgg cgcaatctcc agtgcaactg aagctattaa gtcgggcaga
      601 aaaattatcg tcgcgaaaga taacgaagat gaagtggggc taattaacgg tgaaggatgc
      661 ctgatagccg atcatctgca ggctgtctgc gcgtttctgg aaggtaagca cgctctcgaa
      721 cacccgaaac caactaatgc agtatcccgg gcgctacaac atgatctcag tgatgttgtc
      781 ggtcaggagc aaggaaagcg aggactggaa attaccgccg ctggcgggca caacctttta
      841 ctgattggac cgccgggaac aggtaaaaca atgctcgcca gccgtattaa tggccttttg
      901 ccagatttaa gcaatgaaga ggcactggag agcgctgcga tattaagtct ggtaaatgct
      961 gaatcagtac aaaaacaatg gcggcagcgc ccgttccgct cacctcatca cagtgcgtcg
     1021 ttaactgcga tggtgggcgg tggtgcaatt ccagggccag gtgaaatttc gctggcgcat
     1081 aacggcgtgc tttttcttga tgagctacct gaatttgaac ggcgtacact ggatgccttg
     1141 cgagagccga ttgaatccgg gcagatccat ctttcacgca cacgagcaaa aataacctat
     1201 ccagcccgtt tccagcttgt cgcggcaatg aatcccagcc ctaccggaca ttatcaggga
     1261 aaccataacc gctgcacacc agaacagaca ttacgttatc tcaaccggct ctcggggccc
     1321 tttctcgacc gcttcgatct ctcactggag atcccattac cgccccccgg cattttgagt
     1381 aaaacggtag tgccgggaga aaacagcacc accgttaaac aacgtgtaat ggccgccaga
     1441 gagcgccaat ttaagcggca gaataaactg aacgcctggc tggataatcc ggaaatacgc
     1501 caattctgca agcttgaaag cgaagatgcg cagtggctgg aagaaacgct gatccatctg
     1561 gggttatcga ttcgtgcctg gcagcggtta ttgaaagttg cacgaaccat tgctgatatt
     1621 gatcagtctg acattatcac acgtcagcat ttgcaggagg cagttagcta tcgtgcgatt
     1681 gaccgtttgc tcatccatct gcaaaaacta ctgacataaa aaaagggcat ttcgcccttt
     1741 ttattaatcg tcagaatcgg tgtagtcttc agcaccttca acctgcggtt taccgccgga
     1801 aagggtgtga aaacgttttg gacgcttgat acgcgtcata tacttggacc acacgcgttc
     1861 tgcttctgtc actggttcac gttcgccacg gcatactgct acgaagagtt tctcttcctc
     1921 ggtaaccggc tcgcgtttgc caagatccaa ctcattgaag gcataaccat gacgctcaag
     1981 cagttgtgcc tctttgatgg tgaagtcacc atgacgagag aatccacgtg gataatgttt
     2041 attgtcgaaa tatcgattag tcgtcgtaaa gctttccgcc atcctgcacg ctcctaattc
     2101 tttgaccgag ctagttatgg cgcggagtat tagttacgct tgacagagtg taaaacaaaa
     2161 catttaaatc ataacgacaa ataattttgc ggagagcact gtggatacgg aattgttaaa
     2221 aactttcctg gaagttagcc gaacgcgtca ctttggtcga gcggctgaat cgctctatct
     2281 gacccaatca gccgtgagct ttcgaatcag acaactggaa aatcaactgg gtgtgaatct
     2341 tttcacccgc cacagaaaca atatccgttt aaccgctgcc ggagaaaagc ttcttcctta
     2401 tgcagaaacg ctcatgagca cctggcaggc cgcccgcaag gaggtggcgc atacctcacg
     2461 acataatgag ttttctatcg gtgccagcgc ctcgctgtgg gagtgtatgc ttaatcagtg
     2521 gctgggacgt ttgtatcaaa atcaggatgt ccatacaggc ttacagttcg aagcgcgaat
     2581 tgcccaacgg cagtctctgg taaaacagtt acatgaacgc cagcttgatc ttcttattac
     2641 aactgaagcc cccaaaatgg acgaattttg tagtcagttg ctggggtatt tcactttagc
     2701 gctttatacc agtgcccctt ctaaactaaa gggagatctt aactatctgc gactagagtg
     2761 ggggccagat tttcaacagc atgaggcagg tttgatcggt gctgacgaag ttcccattct
     2821 gacaaccagt tctgctgaac tggcacagca acagattgcg atgcttaatg gttgcacctg
     2881 gctacccgtc agttgggcac gtaaaaaagg cggcctgcat accgttgtcg atagcacaac
     2941 actttcacgg ccgctttatg ccatatggct acaaaatagc gataaaaatg cgttgattcg
     3001 cgatctattg aaaattaacg tgctggatga agtgtattaa tctgaatggc tggcaaggat
     3061 gccggtagaa ggatttactt cggagagggt tatttcagat aaaaaaaatc cttagctttc
     3121 gctaaggatg atttctggca ggggcggaga gactcgaact cccaacaccc ggttttggag
     3181 accggtgctc taccaattga actacgcccc taattagggt ggcggaacgg acgggactcg
     3241 aacccgcgac cccctgcgtg acaggcaggt attctaaccg actgaactac cgctccaccg
     3301 aattctttta caaccaccgg ttttatgacc ggcttac
//
LOCUS       DADSYM010000233         3267 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2415
            SAMN08039579-rid11723173.denovo.235, whole genome shotgun sequence.
ACCESSION   DADSYM010000233 DADSYM010000000
VERSION     DADSYM010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039579
            Sequence Read Archive: SRR6362533
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3267)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3267)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980162.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 03:39:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,484
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 4,347
            CDSs (with protein)               :: 4,347
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 43 of 85
            Pseudo Genes (incomplete)         :: 37 of 85
            Pseudo Genes (internal stop)      :: 19 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3267
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039579-rid11723173.denovo.235"
                     /strain="LGE2415"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8302 N 95.9628 W"
                     /collection_date="2015-08-24"
     gene            301..1617
                     /gene="shiA"
                     /locus_tag="J1Y83_004206"
     CDS             301..1617
                     /gene="shiA"
                     /locus_tag="J1Y83_004206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate transporter"
                     /protein_id="HBA7632286.1"
                     /translation="MDSTLISTRPDEGTLSLSRARRAALGSFAGAVVDWYDFLLYGIT
                     AALVFNREFFPQVSPAMGTLAAFATFGVGFLFRPLGGVIFGHFGDRLGRKRMLMLTVW
                     MMGIATALIGILPSFSTIGWWAPILLVTLRAIQGFAVGGEWGGAALLSVESAPKNKKA
                     FYSSGVQVGYGVGLLLSTGLVSLISMMTTDEQFLSWGWRIPFLFSIVLVLGALWVRNG
                     MEESAEFEQQQHNQAAAKKRIPVIEALLRHPGAFLKIIALRLCELLTMYIVTAFALNY
                     STQNMGLPRELFLNIGLLVGGLSCLTIPCFAWLADRFGRRRVYITGALIGTLSAFPFF
                     MALEAQSIFWIVFFSIMLANIAHDMVVCVQQPMFTEMFGASYRYSGAGVGYQVASMVG
                     GGFTPFIAAALITYFAGNWHSVAIYLLAGCLISAMTALLMKDNQRS"
     gene            1719..3173
                     /gene="amn"
                     /locus_tag="J1Y83_004207"
     CDS             1719..3173
                     /gene="amn"
                     /locus_tag="J1Y83_004207"
                     /EC_number="3.2.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP nucleosidase"
                     /protein_id="HBA7632287.1"
                     /translation="MNNKGSGLTPAQALDKLDALYEQSVVALRNAIGKYITSGELPDE
                     NARKQGLFVYPSLTVTWDGSTTNPPKTRAFGRFTHAGSYTTTITRPTLFRSYLNEQLT
                     LLYQDYGAHISVQPSQHEIPYPYVIDGSELTLDRSMSAGLTRYFPTTELAQIGDETAD
                     GIYHPTEFSPLSHFDARRVDFSLARLRHYTGTPVEHFQPFVLFTNYTRYVDEFVRWGC
                     SQILDPDSPYIALSCAGGNWITAETEAPEEAISDLAWKKHQMPAWHLITADGQGITLV
                     NIGVGPSNAKTICDHLAVLRPDVWLMIGHCGGLRESQAIGDYVLAHAYLRDDHVLDAV
                     LPPDIPIPSIAEVQRALYDATKLVSGRPGEEVKQRLRTGTVVTTDDRNWELRYSASAL
                     RFNLSRAVAIDMESATIAAQGYRFRVPYGTLLCVSDKPLHGEIKLPGQANRFYEGAIS
                     EHLQIGIRAIDLLRAEGDRLHSRKLRTFNEPPFR"
BASE COUNT          750 a          794 c          793 g          930 t
ORIGIN      
        1 ccctactggt tataatgcaa tgcagtctat cagaatcatc agggtgcaat tttgtgcata
       61 tgcggacttt tatgtttcat atctcgaacc tgtgggccct ctgcttaatc cttaaacaac
      121 accagcaact cctgcgcttt catcttccat cgaatttttc atgttgccgc taatcagcca
      181 taaaatcatt tgcagatgcg ctctgtcgag gtagtctcat aaggttcgtt tatagatcga
      241 cggcaatgtg agttaccttt tccatactaa ttataaaaag acagtacaaa caggatcatt
      301 atggactcca cgctcatctc cactcgtccc gatgaaggga cgctttcgtt aagtcgcgcc
      361 cgacgagctg cgttaggcag tttcgctggt gccgtcgtcg actggtatga ttttttactc
      421 tatggcatta ccgccgcact ggtgtttaat cgcgagtttt tcccgcaagt aagcccggcg
      481 atgggaacgc tcgccgcatt tgccaccttt ggtgtcggct tccttttccg accgctcggc
      541 ggtgtcattt tcggtcactt tggtgaccgg ctgggacgta agcgcatgtt aatgctgacc
      601 gtctggatga tgggcatcgc gacagccttg attggtattc ttccttcatt ctcgaccatt
      661 gggtggtggg cacctatttt gctggtgaca ctgcgtgcca ttcagggatt tgccgtcggc
      721 ggcgaatggg gaggcgcagc gttgctttcc gttgaaagtg cgccgaaaaa taaaaaagcc
      781 ttttacagta gcggtgtaca agttggctac ggagtaggtt tactgctttc aaccggactg
      841 gtttcattga tcagtatgat gacgactgac gaacagtttt taagctgggg ctggcgcatt
      901 cctttcctgt ttagcatcgt actggtactg ggagcattgt gggtgcgcaa tggcatggag
      961 gagtccgcgg aatttgaaca acagcaacat aatcaagcag ccgcgaaaaa acgcatcccg
     1021 gttatcgaag ctctgttacg acatcccggt gctttcctga agattattgc actacgactg
     1081 tgcgagttgc tgacgatgta cattgttact gcctttgcac ttaattattc aacccagaat
     1141 atggggttac cgcgcgaact tttccttaat attggtttgc tggtaggtgg attaagctgc
     1201 ctgacaattc cctgttttgc ctggcttgcc gatcgttttg gtcggcgcag ggtttatatc
     1261 acaggcgcgt tgatcggaac gttgagcgca tttcctttct ttatggcgct tgaagcacaa
     1321 tctattttct ggatagtttt cttctccata atgctggcaa acattgcgca tgacatggtg
     1381 gtgtgtgtgc aacaaccgat gtttaccgaa atgtttggtg ccagttatcg ctacagtggt
     1441 gctggagtcg gttatcaggt tgccagtatg gttggcggtg gatttacacc ttttattgcc
     1501 gctgcactca tcacttactt tgccgggaac tggcatagcg tcgccattta tttgctggct
     1561 ggatgcctga tttctgcaat gaccgcttta ttgatgaaag acaatcaacg ctcgtgatag
     1621 cctggcgaag atcatccgat cttcgcctta tacttttgtt tcacattcct gtgacatact
     1681 atcggatgtg cggtaattgt atggaacagg agacacacat gaataataag ggctccggtc
     1741 tgaccccagc tcaggcactg gataaactcg acgcgctgta tgagcaatct gtagttgctt
     1801 tacgcaacgc cattggcaaa tatattacaa gtggcgaatt acctgatgaa aacgcccgca
     1861 aacaaggtct ttttgtctat ccatcactga ccgtaacctg ggacggtagc acaactaatc
     1921 cccccaaaac gcgcgcattt ggtcgtttta ctcacgcagg cagctacacc accacgatta
     1981 ctcgccctac tctctttcgt tcgtatctca atgaacaact tacgttgctg tatcaggatt
     2041 atggtgcgca tatctctgtg caaccctcgc agcatgaaat cccttatcct tatgtcatcg
     2101 atggctctga attgacactt gatcgctcaa tgagcgctgg tttaactcgc tacttcccga
     2161 caacagaact ggcgcaaatc ggcgatgaaa ctgcagacgg catttatcat ccaacggaat
     2221 tctccccgtt atcgcatttt gatgctcgcc gggttgattt ttccctcgca cggttgcgcc
     2281 attataccgg tacgccagta gaacattttc aaccgttcgt cttgtttacc aactacacac
     2341 gttatgtgga tgaattcgtt cgttggggat gcagccagat cctcgatcct gatagtccct
     2401 acattgccct ttcttgtgct ggcgggaact ggatcactgc cgaaaccgaa gcgccagaag
     2461 aagccatttc cgaccttgca tggaaaaaac accagatgcc agcatggcat ttaattaccg
     2521 ctgatggtca gggtattact ctggtgaata ttggcgtggg accgtccaat gctaaaacca
     2581 tctgcgatca tctggcagtg ctacgcccgg atgtctggtt gatgattggt cactgtggcg
     2641 gattacgtga aagccaggcc attggagatt atgtccttgc acacgcttat ttacgcgatg
     2701 accacgttct tgatgcggtt ctgccgcccg atattcctat tccgagcatt gctgaagtgc
     2761 aacgtgcgct ttatgacgct accaagttag tcagcggcag gcctggtgag gaagtcaaac
     2821 agcggctgcg tacaggtact gtggtaacca cagatgacag gaactgggag ttacgttact
     2881 cagcttctgc acttcgtttt aacttaagcc gggccgtagc aattgatatg gaaagtgcaa
     2941 ccattgccgc gcaaggatat cgtttccgcg tgccatacgg gacactactg tgtgtttcag
     3001 ataaaccgtt acatggtgag attaagcttc ccggccaggc taaccgtttt tatgaaggcg
     3061 ctatttccga acacctgcaa attggcattc gggcgatcga tttgctgcgc gcagaaggcg
     3121 accgactgca ttcacgtaaa ttacgaacct ttaatgagcc gccgttccga taataagtta
     3181 tgaaccttct tatcagaaat agatgtgaag gattaagtaa gacatctggc aaatagcctg
     3241 caataaacgc gggctgtttg cttcctt
//
LOCUS       DADSYM010000234         3229 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2415
            SAMN08039579-rid11723173.denovo.236, whole genome shotgun sequence.
ACCESSION   DADSYM010000234 DADSYM010000000
VERSION     DADSYM010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039579
            Sequence Read Archive: SRR6362533
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3229)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3229)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980162.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 03:39:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,484
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 4,347
            CDSs (with protein)               :: 4,347
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 43 of 85
            Pseudo Genes (incomplete)         :: 37 of 85
            Pseudo Genes (internal stop)      :: 19 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3229
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039579-rid11723173.denovo.236"
                     /strain="LGE2415"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8302 N 95.9628 W"
                     /collection_date="2015-08-24"
     gene            174..1277
                     /gene="proB"
                     /locus_tag="J1Y83_004208"
     CDS             174..1277
                     /gene="proB"
                     /locus_tag="J1Y83_004208"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HBA7632288.1"
                     /translation="MSDSQTLVVKLGTSVLTGGSRRLNRAHIVELVRQCAQLHAAGHR
                     IVIVTSGAIAAGREHLGYPELPATIASKQLLAAVGQSRLIQLWEQLFSIYGIHVGQML
                     LTRADMEDRERFLNARDTLRALLDNNIVPVINENDAVATAEIKVGDNDNLSALAAILA
                     GADKLLLLTDQKGLYTADPRSNPQAELIKDVYGIDDALRAIAGDSVSGLGTGGMSTKL
                     QAADVACRAGIDTIIAAGSKPGVIGDVMEGISVGTLFHAQATPLENRKRWIFGAPPAG
                     EITVDEGATAAILERGSSLLPKGIKSVTGNFSRGEVIRICNLEGRDIAHGVSRYNSDA
                     LRRIAGHHSQEIDAILGYEYGPVAVHRDDMITR"
     gene            1289..2542
                     /gene="proA"
                     /locus_tag="J1Y83_004209"
     CDS             1289..2542
                     /gene="proA"
                     /locus_tag="J1Y83_004209"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HBA7632289.1"
                     /translation="MLEQMGIAAKQASYKLAQLSSREKNRVLEKIADELEAQSESILN
                     ANAQDVADARANGLSEAMLDRLALTPARLKDIADDVRQVCNLADPVGQVIDGGVLDSG
                     LRLERRRVPLGVIGVIYEARPNVTVDVASLCLKTGNAVILRGGKETCRTNAATVAVIQ
                     DALKSCGLPAGAVQAIDNPDRALVSEMLRMDKYIDMLIPRGGAGLHKLCREQSTIPVI
                     TGGIGVCHIYVDESAEIAEALKVIVNAKTQRPSTCNTVETLLVNKNIADSFLPALSKQ
                     MAESGVTLHADAAALAQLQAGPAKVVAVKAEEYDDEFLSLDLNVKIVSDLDDAIAHIR
                     EHGTQHSDAILTRDMRNAQRFVNEVDSSAVYVNASTRFTDGGQFGLGAEVAVSTQKLH
                     ARGPMGLEALTTYKWIGIGDYTIRA"
     gene            2657..2732
                     /locus_tag="J1Y83_004210"
     tRNA            2657..2732
                     /locus_tag="J1Y83_004210"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2690..2692,aa:Thr,seq:cgt)
     gene            2887..3225
                     /locus_tag="J1Y83_004211"
     CDS             2887..3225
                     /locus_tag="J1Y83_004211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001545410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4102 domain-containing protein"
                     /protein_id="HBA7632290.1"
                     /translation="MKLNARQIDTAKPKEKAYKLADGGGLYLLVKPGGGEYWRLKYRV
                     AGKEKLLALGVYPEVTLADAPAKLEEAKRGISGGIDLMEVKREEKIARETQLNNTFKD
                     IALEWHSNKL"
BASE COUNT          805 a          790 c          897 g          737 t
ORIGIN      
        1 aaaatgtggt aatttattaa atctgtaata aaagcgtaaa caactgccgc tacgcttgct
       61 gatcccgcgc aacaaaacgc catgctttgc tcgcagatgg ttggcaaccg acgacagtcc
      121 tgctaaaacg ttcgtttgat atcatttttc ctaaaattga atggcagaga atcatgagtg
      181 acagccagac gctggtggta aaactcggca ccagtgtgct aacaggcgga tcgcgccgcc
      241 tgaaccgtgc ccatatcgtt gaacttgttc gccagtgcgc gcagttacat gccgccgggc
      301 atcggattgt tattgtgacg tcgggtgcga ttgccgccgg acgtgagcac ctggggtacc
      361 cggaattgcc agcgactatc gcttcaaaac agctgctggc ggcggtaggg cagagtcgac
      421 tgattcaact gtgggaacag ctgttttcaa tttacggcat tcatgtcggg cagatgttgc
      481 tgacccgtgc tgatatggaa gatcgtgaac gcttcctgaa cgcccgtgat accctgcgtg
      541 cgttgctcga taacaatatc gttccggtaa ttaatgagaa cgatgctgtc gctactgcag
      601 agattaaggt cggtgataac gataaccttt ctgcgctggc ggcgattctg gcgggtgccg
      661 ataaactgtt gctgctgacc gatcaaaaag gtttgtatac cgctgacccg cgcagcaatc
      721 cgcaggcaga actgattaaa gatgtgtacg gcattgatga cgcactgcgc gcgattgccg
      781 gtgatagcgt ttcaggcctc ggaactggcg gcatgagcac caaattgcag gccgcggacg
      841 tggcttgccg tgcgggtatc gacaccatta ttgccgcggg cagcaagccg ggcgttattg
      901 gtgatgtgat ggaaggcatt tccgtcggta cgctgttcca tgcccaggcg actccgcttg
      961 aaaaccgtaa acgctggatt ttcggtgcgc cgcctgcggg tgaaatcacg gtagatgaag
     1021 gggcaactgc cgccattctg gaacgcggca gctccctgtt gccgaaaggc attaaaagcg
     1081 tgactggcaa tttctcgcgt ggtgaagtca tccgtatttg caacctcgaa ggtcgcgata
     1141 tcgcccacgg cgtcagtcgt tacaacagcg atgcattacg ccgtattgcc ggacaccact
     1201 cgcaagaaat tgatgcaata ctgggatatg aatacggccc ggttgccgtt caccgtgatg
     1261 acatgattac ccgttaagga gctggccgat gctggaacaa atgggcattg ccgcgaagca
     1321 agcctcgtat aaattagcgc aactctccag ccgcgaaaaa aatcgtgtgc tggaaaaaat
     1381 cgccgatgaa ctggaagcac aaagcgaaag tatcctcaac gctaacgcgc aggatgttgc
     1441 agacgcgcgt gccaatggcc ttagcgaagc gatgcttgac cgtctggcac tgacgcccgc
     1501 acggctgaaa gacattgccg acgatgtgcg ccaggtgtgc aatctcgccg atccggtagg
     1561 gcaggtaatc gatggcggcg tactggacag cggcctgcgt cttgagcgtc gtcgcgtacc
     1621 gctgggggtg attggcgtga tttatgaagc gcgcccgaac gtgacggttg atgttgcttc
     1681 cctgtgcctg aaaaccggta acgcggtgat cctgcgcggc ggcaaagaaa cctgtcgcac
     1741 taacgctgca acggtagcgg tgattcagga cgccctgaaa tcctgcggct taccggcggg
     1801 tgccgtgcag gcaattgata atcctgaccg tgcgctggtc agtgaaatgc tgcgtatgga
     1861 taaatacatc gacatgctga tcccgcgcgg tggggctggt ttgcataaac tgtgccgcga
     1921 gcagtcgaca atcccggtga tcacaggcgg tataggcgta tgccatattt acgttgatga
     1981 aagcgcagag atcgctgaag cattaaaagt gatcgtcaac gcgaaaactc agcgaccgag
     2041 cacatgtaat acggtagaaa cgttgctggt aaataaaaac atcgcagata gcttcctgcc
     2101 cgcattaagc aaacaaatgg cggaaagcgg cgtgacgtta cacgctgatg cagccgcgct
     2161 ggcgcagttg caggcaggcc ccgcgaaggt ggtggctgtt aaagccgaag agtatgacga
     2221 tgagtttctg tcattagatt tgaacgtcaa aatcgttagc gatctggatg acgccattgc
     2281 ccatattcgt gaacacggca cgcaacactc cgatgcgatc ctgacccgcg atatgcgcaa
     2341 cgcccagcgt tttgttaacg aagtggattc ctccgctgtt tacgttaacg cctctacgcg
     2401 ttttaccgac ggcggccagt ttggactggg ggcggaagtt gcggtaagca cacaaaaact
     2461 ccacgcgcgc ggcccaatgg ggctggaagc actgaccact tataagtgga tcggcattgg
     2521 tgattacacc attcgtgcgt aaataaaacc gggtgatgca aaagtagcca tttgattcac
     2581 aaggccattg acgcatcgcc cggttagttt taaccttgtc caccgtgatt cacgttcgtg
     2641 aacatgtcct ttcagggccg atatagctca gttggtagag cagcgcattc gtaatgcgaa
     2701 ggtcgtaggt tcgactccta ttatcggcac cattctaacg tctccccaag tctactcaag
     2761 tatttaaaaa cctcttataa tcagtatatt aatgcccctt ttagtctttt gacgtctatt
     2821 taagtacccc aaactctaca agcaattgag gggatttgct gttcagttta gtggagatac
     2881 ccccaagtga aactcaatgc ccgtcaaata gacactgcca agccaaaaga gaaggcttac
     2941 aagttggctg atggtggtgg tctgtacctc ctggtaaaac ctggtggagg agaatattgg
     3001 cgtctcaagt atcgtgtagc tggtaaagag aagctgttag cactaggtgt gtatcctgag
     3061 gtcaccttag ctgatgctcc tgcaaaactt gaagaagcta aaagaggtat ctctggggga
     3121 atcgatctga tggaagtgaa gcgagaggaa aagattgccc gggaaacgca gttaaacaac
     3181 accttcaaag atattgccct tgagtggcac agtaacaaat tataatcac
//
LOCUS       DADSYM010000235         3207 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2415
            SAMN08039579-rid11723173.denovo.237, whole genome shotgun sequence.
ACCESSION   DADSYM010000235 DADSYM010000000
VERSION     DADSYM010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039579
            Sequence Read Archive: SRR6362533
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3207)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3207)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980162.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 03:39:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,484
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 4,347
            CDSs (with protein)               :: 4,347
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 43 of 85
            Pseudo Genes (incomplete)         :: 37 of 85
            Pseudo Genes (internal stop)      :: 19 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3207
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039579-rid11723173.denovo.237"
                     /strain="LGE2415"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8302 N 95.9628 W"
                     /collection_date="2015-08-24"
     gene            complement(252..1616)
                     /gene="ppnN"
                     /locus_tag="J1Y83_004212"
     CDS             complement(252..1616)
                     /gene="ppnN"
                     /locus_tag="J1Y83_004212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide 5'-monophosphate nucleosidase"
                     /protein_id="HBA7632291.1"
                     /translation="MITHISPLGSMDMLSQLEVDMLKRTASSDLYQLFRNCSLAVLNS
                     GSLTDNSKELLSRFENFDINVLRRERGVKLELINPPEEAFVDGRIIRALQANLFAVLR
                     DILFVYGQIHNTVRFPNLNLDNSVHITNLVFSILRNARALHVGEAPNMVVCWGGHSIN
                     ENEYLYARRVGNQLGLRELNICTGCGPGAMEAPMKGAAVGHAQQRYKDSRFIGMTEPS
                     IIAAEPPNPLVNELIIMPDIEKRLEAFVRIAHGIIIFPGGVGTAEELLYLLGILMNPA
                     NKDQVLPLILTGPKESADYFRVLDEFVVHTLGENARRHYRIIIDDAAEVARQMKKSMP
                     LVKENRRDTGDAYSFNWSMRIAPDLQMPFEPSHENMANLKLYPDQPVEVLAADLRRAF
                     SGIVAGNVKEVGIRAIEEFGPYKINGDKEIMRRMDDLLQGFVAQHRMKLPGSAYIPCY
                     EICT"
     gene            complement(1728..2576)
                     /gene="queF"
                     /locus_tag="J1Y83_004213"
     CDS             complement(1728..2576)
                     /gene="queF"
                     /locus_tag="J1Y83_004213"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 7-cyano-7-deazaguanine reductase
                     QueF"
                     /protein_id="HBA7632292.1"
                     /translation="MSSYANHQALAGLTLGKSTDYRDTYDASLLQGVPRSLNRDPLGL
                     KADNLPFHGTDIWTLYELSWLNAKGLPQVAVGHVELDYTSVNLIESKSFKLYLNSFNQ
                     TRFNNWDEVRQTLERDLSTCAQGKVSVALYRLDELEGQPIGHFNGTCIDDQDITIDNY
                     EFTTDYLENATSGEKVVEETLVSHLLKSNCLITHQPDWGSIQIQYRGRQIDREKLLRY
                     LVSFRHHNEFHEQCVERIFNDLLRFCQPEKLSVYARYTRRGGLDINPWRSNSDFVPST
                     TRLVRQ"
     gene            2644..3189
                     /gene="syd"
                     /locus_tag="J1Y83_004214"
     CDS             2644..3189
                     /gene="syd"
                     /locus_tag="J1Y83_004214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SecY-interacting protein"
                     /protein_id="HBA7632293.1"
                     /translation="MDDLTAQALKDFTARYCDAWHEEHKSWPLSEELYGVPSPCIIST
                     TEDAVYWQPQPFTGEQNVNAVERAFDIVIQPTIHTFYTTQFAGDMHAQFGDIKLTLLQ
                     TWSEDDFRRVQENLIGHLVTQKRLKLPPTLFIATLEEELEVISVCNLSGEVCKETLGT
                     RKRTHLASNLAEFLNQLKPLL"
BASE COUNT          862 a          766 c          784 g          795 t
ORIGIN      
        1 gtaggttgaa ctgaaaaaat caatactttt agttaatcat ggcgataatt tctttttcat
       61 ggcgataaaa tgtagtttat gaaaataggg aaacgtttgc gtttcaacat aaattgtatg
      121 acatattaac tgtggtgata tttgtcgcgt gagggtggta ggaaacattc atataacaaa
      181 tgaaatgagt tgatgagtaa gtgaccaaaa acgagaaaac ccggcaacaa acgtcaccgg
      241 gggaggggag attatgtgca gatttcgtag caagggatgt aggctgagcc tggcaacttc
      301 atacgatgct gggcaacaaa accctgtagc aggtcgtcca tacgacgcat aatctcttta
      361 tcgccgttga ttttgtaagg accaaactct tcaatggcgc gaataccgac ttcttttaca
      421 ttacccgcca caataccgga gaacgcacgg cgcaggtcgg cagccagcac ttcaacgggt
      481 tgatccgggt aaagcttcag attagccata ttctcgtgag acggctcaaa cggcatttgc
      541 aaatctggcg caatgcgcat tgaccagtta aagctgtaag cgtcaccagt gtcgcggcga
      601 ttttctttca ccagcggcat cgattttttc atctgacgag cgacttcagc ggcgtcatca
      661 atgatgatgc ggtaatggcg gcgcgcgttt tcacccagcg tatgcacgac aaactcgtcc
      721 agtacgcgga agtagtcggc gctctctttc gggccggtga ggatcaatgg taaaacctga
      781 tctttgttgg ccgggttcat taaaattccc agcaaataca gcaactcttc tgctgtaccc
      841 acaccgccag ggaagataat gataccgtga gcgatacgga caaacgcttc cagacgtttt
      901 tcgatatctg gcatgatgat caattcgttg accagcgggt taggcggttc agcggcgata
      961 atcgacggct ctgtcatacc aataaagcga ctgtctttgt aacgctgctg cgcgtgtccg
     1021 accgcagcac ctttcatcgg cgcttccatc gctcccggac cacagccggt gcagatattc
     1081 agctcacgca ggcccagctg attcccgacg cgacgggcat acaaatactc gttttcgtta
     1141 attgagtgac cgccccagca gaccaccata tttggcgctt caccgacatg cagcgcgcga
     1201 gcgttacgca agatggaaaa gaccaggtta gtgatgtgga cggagttgtc gagattcagg
     1261 ttgggaaaac gaacggtgtt atgaatttgc ccataaacga agagaatgtc acgcagaacc
     1321 gcgaacaagt tggcctgcaa agcgcgaata attcgcccat cgacaaaagc ctcttccggg
     1381 ggatttatca gttccagctt tacgccgcgt tcacggcgca ggacattaat atcgaaattt
     1441 tcaaaacgag acagcaattc tttgctgtta tcggtcaaac taccggagtt cagtacggca
     1501 agtgaacagt tgcgaaacag ttgatagagg tcgctgctgg cggtgcgttt aagcatatcc
     1561 acttccagct gcgacaacat atccatggag ccaagcgggc taatatgtgt aatcaagaaa
     1621 aactccttat gggacgaata tcgtcattcc ctttgattac aatagccctg cctgctgacc
     1681 tttcacaacc ttctgcgtga atccagcacg caaaattgag aaaaaattta ttgccgaacc
     1741 agtcttgtgg tcgatgggac aaaatcgcta ttactgcgcc acgggttaat gtccagaccg
     1801 ccacgacggg tataacgtgc gtaaacgctc aatttctctg gctggcagaa gcgtaacagg
     1861 tcattaaaga tgcgttccac gcactgttcg tggaactcgt tgtgatgacg gaaagaaacc
     1921 aggtaacgca gcagtttttc tctgtcaatt tggcgtccac gatactgaat ttggatcgat
     1981 ccccaatctg gttgatgggt gatcaggcaa tttgatttca gcaggtggct gacaagcgtc
     2041 tcttccacta ctttttcacc gctggtggca ttctccagat agtcagtagt gaattcatag
     2101 ttatcgatag tgatatcctg gtcatcaatg caggtgccat tgaaatgacc tatcggctgg
     2161 ccttccagtt catcaagacg atataacgcc acgctaacct taccctgggc gcaagtgctt
     2221 aagtcgcgct ccagcgtctg gcgcacctca tcccagttat taaaacgcgt ctggttaaaa
     2281 ctgttgagat agagcttaaa actcttcgac tcaatcagat ttacgctggt gtaatcaagt
     2341 tcaacatgac cgacagcgac ctgcggcaaa cctttcgcat tcagccagga aagttcatac
     2401 agcgtccaga tatccgtacc gtgaaaaggc aggttatccg ctttcagacc cagcgggtcg
     2461 cgattcaggc tgcgtggtac gccttgcagt aggctggcgt cataggtatc ccggtaatcg
     2521 gttgattttc caagtgtcag gcccgcaagt gcctgatggt ttgcataaga agacatgttt
     2581 caccgtcata aaccagataa actaaacgac aagtgtatct ggtttaagtg aagagagaaa
     2641 ttagtggacg atttgaccgc acaagccctg aaagatttta ctgcacgcta ctgcgatgcg
     2701 tggcatgaag agcataaaag ctggccgtta agtgaggaac tgtacggcgt tccttcaccg
     2761 tgcattattt caactaccga agatgccgta tactggcaac cccagccgtt taccggggaa
     2821 caaaatgtaa acgcggttga acgcgctttt gatattgtga tacaacccac gattcatacc
     2881 ttttatacga ctcagtttgc cggggatatg cacgcgcagt ttggcgatat caaactgaca
     2941 ttgctgcaaa cctggagtga agacgacttc cgtcgggtgc aggaaaatct gattggtcat
     3001 ctggtgactc agaaaaggct aaaactgccg cccacgctat ttatcgcgac gctggaagaa
     3061 gagttagagg tcatctcggt gtgtaatctc tccggtgaag tgtgcaaaga gacgctagga
     3121 acacgtaaac ggacgcattt agcctcaaat cttgcggaat tccttaatca acttaagcct
     3181 cttctgtaat ccaaataccc catctgg
//
LOCUS       DADSYM010000236         3189 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2415
            SAMN08039579-rid11723173.denovo.238, whole genome shotgun sequence.
ACCESSION   DADSYM010000236 DADSYM010000000
VERSION     DADSYM010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039579
            Sequence Read Archive: SRR6362533
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3189)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3189)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980162.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 03:39:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,484
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 4,347
            CDSs (with protein)               :: 4,347
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 43 of 85
            Pseudo Genes (incomplete)         :: 37 of 85
            Pseudo Genes (internal stop)      :: 19 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3189
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039579-rid11723173.denovo.238"
                     /strain="LGE2415"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8302 N 95.9628 W"
                     /collection_date="2015-08-24"
     gene            complement(67..594)
                     /gene="mobB"
                     /locus_tag="J1Y83_004215"
     CDS             complement(67..594)
                     /gene="mobB"
                     /locus_tag="J1Y83_004215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein B"
                     /protein_id="HBA7632294.1"
                     /translation="MAGKTMIPLLAFAAWSGTGKTTLLKKLIPALCARGIRPGLIKHT
                     HHDMDVDKPGKDSYELRKAGAAQTIVASQQRWALMTETPDEEELDLHFLASRMDTSKL
                     DLILVEGFKHEEIAKIVLFRDGAGHRPEELVIDRHVIAVASDVPLNLDVALLDINDVE
                     GLADFVVEWMQKQDG"
     gene            complement(576..1160)
                     /gene="mobA"
                     /locus_tag="J1Y83_004216"
     CDS             complement(576..1160)
                     /gene="mobA"
                     /locus_tag="J1Y83_004216"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase MobA"
                     /protein_id="HBA7632295.1"
                     /translation="MNLMTTITGVVLAGGKARRMGGVDKGLLELNGKPLWQHVADALM
                     TQLSHVVVNANRHQEIYQVSGLKVIEDSLADYPGPLAGMLSVMQQEAGEWFLFCPCDT
                     PYIPHDLAARLTHQRKDAPVVWVHDGERDHPTIALVNRAIEPLLLEYLQAGERRVMAF
                     MRLAGGHAVDFSDRKEAFINVNTPEELARWQEKR"
     gene            1230..1499
                     /locus_tag="J1Y83_004217"
     CDS             1230..1499
                     /locus_tag="J1Y83_004217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihD family protein"
                     /protein_id="HBA7632296.1"
                     /translation="MKCKRLNEVIELLQPAWQKEPDLNLLQFLQKLAKESGFDGELAD
                     LTDDILIYHLKMRDSAKDAVIPGLQKDYEEDFKTALLRARGVIKE"
     gene            1576..2562
                     /gene="srkA"
                     /locus_tag="J1Y83_004218"
     CDS             1576..2562
                     /gene="srkA"
                     /locus_tag="J1Y83_004218"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response kinase SrkA"
                     /protein_id="HBA7632297.1"
                     /translation="MNNSAFTFQTLHPDTIMDALFEHGIRVDSGLTPLNSYENRVYQF
                     QDEDRRRFVVKFYRPERWTADQILEEHQFALQLVNDEVPVAAPVAFNGQTLLNHQGFY
                     FAVFPSVGGRQFEADNIDQMEAVGRYLGRMHQTGRKQLFIHRPTIGLNEYLIEPRKLF
                     EDATLIPSGLKAAFLKATDELIAAVTAHWREDFTVLRLHGDCHAGNILWRDGPMFVDL
                     DDARNGPAVQDLWMLLNGDKAEQRMQLETIIEAYEEFSEFDTAEIGLIEPLRAMRLVY
                     YLAWLMRRWADPAFPKNFPWLTGEDYWLRQTATFIEQAKVLQEPPLQLTPMY"
     gene            2579..>3189
                     /gene="dsbA"
                     /locus_tag="J1Y83_004219"
     CDS             2579..>3189
                     /gene="dsbA"
                     /locus_tag="J1Y83_004219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbA"
                     /protein_id="HBA7632298.1"
                     /translation="MKKIWLALAGLVLAFSASAAQYEDGKQYTTLEKPVAGAPQVLEF
                     FSFFCPHCYQFEEVLHISDNVKKKLPEGVKMTKYHVNFMGGDLGKELTQAWAVAMALG
                     VEDKVTVPLFEGVQKTQTIRSASDIRDVFINAGIKGEEYDAAWNSFVVKSLVAQQEKA
                     AADVQLRGVPAMFVNGKYQLNPQGMDTSNMDVFVQQYADTVKYL"
BASE COUNT          800 a          773 c          780 g          836 t
ORIGIN      
        1 tttttgcgtt ggtgcggaat aaatgccgga tgcgacgctg gcgcgtttgc tccagcctac
       61 tcttgcttat ccgtcctgct tttgcatcca ctccaccaca aaatcagcca gcccctcaac
      121 atcattaata tccagtaacg cgacatcaag attaagcggc acatcactgg ctacagcaat
      181 aacatgcctg tctatcacta attcttccgg tcgatgtccg gctccatcgc gaaacagcac
      241 aatctttgcg atctcttcat gcttaaaccc ttcgaccaga atcaaatcca gctttgaggt
      301 atccattcga cttgcgagaa aatgtagatc cagctcttct tcgtctggtg tttccgtcat
      361 caaggcccat cgctgttggc tggcaacgat ggtttgcgcc gcgccagcct tgcgcaactc
      421 atagctatct ttgcccggtt tatcaacatc catatcatgg tgcgtatgct taatcagccc
      481 cggacggatc cctctggcgc ataatgccgg gatcaatttt ttcagcagcg ttgtttttcc
      541 ggtgccactc catgcagcaa aggcgagtaa cggtatcatc gtttttcctg ccatcgggca
      601 agctcctctg gcgtattcac gtttataaat gcctctttac gatcgctgaa atcaaccgca
      661 tgaccgccag ccagacgcat aaatgccata acccggcgtt ctcctgcttg cagatattcc
      721 agtaataaag gctcaattgc gcggtttacc agagcaatag tcgggtgatc gcgttcaccg
      781 tcgtggaccc acacgacagg cgcatctttg cgctgatgag taagccgggc ggctaaatca
      841 tggggaatgt aaggcgtatc gcacgggcaa aacaaaaacc actcacccgc ttcctgctgc
      901 attactgaaa gcattcctgc cagaggacca gggtaatccg ccagtgaatc ttcaatcact
      961 ttcagaccgc ttacctgata gatttcctga tgacgattag cattaaccac gacgtgagaa
     1021 agctgcgtca taagcgcgtc agcgacatgc tgccataatg gtttgccgtt taattcaagc
     1081 aatcctttat ctacgccgcc cattcgtctg gctttaccgc ccgccagcac aacgcctgtt
     1141 atcgtcgtca tcagattcac cgatatcgcc tcttttattg tgggattgac cctgctaacg
     1201 tgtctgtctc aagagtaagg agcattacta tgaaatgtaa acgtctgaat gaagttattg
     1261 aactcctcca gccagcctgg caaaaagagc cagaccttaa cctgctgcaa tttttgcaga
     1321 aactggcgaa agagtcaggt tttgacggcg aactggcgga tttgacggat gacattctga
     1381 tctatcacct gaaaatgcgt gattccgcga aagatgcggt gatcccgggt ttgcagaaag
     1441 attatgaaga agatttcaaa acggcgctat tacgcgcacg tggtgtaatt aaagagtaaa
     1501 agcttgtaag cggcgcaacc aaaatcatcg tgaaatgata tccttcatca ttcgtaatgt
     1561 tttccggatg atgggatgaa caacagcgct tttactttcc agacactaca cccggatacc
     1621 atcatggacg ctctgtttga gcatgggatt cgggtggatt ccggtcttac cccgcttaac
     1681 agctatgaaa accgtgtcta tcaatttcag gacgaagatc gtcgacgttt tgtcgtcaaa
     1741 ttttatcgcc ctgaacgttg gacagccgat caaatcctcg aagaacatca atttgcgttg
     1801 cagctggtta acgatgaagt tccggtcgca gcacctgtgg cctttaatgg tcagacttta
     1861 ttgaaccatc agggatttta tttcgctgtt tttccaagcg tcggtggtcg ccagttcgaa
     1921 gctgataata tcgatcagat ggaagcggtt gggcgttatt tagggcgtat gcaccagacg
     1981 gggcgcaaac agctttttat ccatcgcccg accatcggtt tgaatgaata tctcattgag
     2041 ccacgcaagc tgtttgagga cgctacacta ataccttccg ggttgaaagc ggcattcctg
     2101 aaagcgacag atgagctgat tgccgccgtt acagcacact ggcgggaaga tttcaccgtt
     2161 ctgcggctac atggagactg ccacgccggg aatattctct ggcgcgatgg tccaatgttt
     2221 gttgatctgg atgatgcacg taatggtcca gccgttcagg atttgtggat gttgctcaat
     2281 ggtgataaag ccgagcagcg gatgcaactg gaaactatta ttgaggctta tgaagaattt
     2341 agcgagttcg acaccgctga aatcggactg attgaacctt tacgcgccat gcgtttggtt
     2401 tattatcttg cctggctaat gcggcgttgg gctgatcccg cgttcccgaa aaatttcccg
     2461 tggttaaccg gggaagatta ctggctacga cagacggcga cttttataga acaggcaaaa
     2521 gttctacaag aacccccttt gcaattaaca cctatgtatt aatcggagag agtagatcat
     2581 gaaaaagatt tggctggcgc tggctggttt agttttagcg tttagcgcat cggcggcgca
     2641 gtatgaagat ggtaaacagt acactaccct ggaaaaacca gttgctggcg cgccgcaagt
     2701 gctggagttt ttctctttct tctgcccgca ctgctatcag tttgaagaag ttctgcatat
     2761 ttctgataac gtgaagaaaa aactgccgga aggcgtgaag atgactaaat accacgtcaa
     2821 cttcatgggg ggtgacctgg gcaaagagct gactcaggca tgggctgtgg cgatggcgct
     2881 gggcgtggaa gacaaagtca cagttccgct gtttgaaggc gtacaaaaaa cccagaccat
     2941 tcgttcagca tctgatatcc gcgatgtatt tatcaacgca ggtattaaag gtgaagagta
     3001 cgacgcggcg tggaacagct tcgtggtgaa atctctggtc gctcagcagg aaaaagctgc
     3061 agctgacgtg caattgcgtg gtgttccggc gatgtttgtt aacggtaaat atcagctgaa
     3121 tccgcagggt atggatacca gcaatatgga tgtttttgtt cagcagtatg ctgatactgt
     3181 gaaatatct
//
LOCUS       DADSYM010000237         3147 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2415
            SAMN08039579-rid11723173.denovo.239, whole genome shotgun sequence.
ACCESSION   DADSYM010000237 DADSYM010000000
VERSION     DADSYM010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039579
            Sequence Read Archive: SRR6362533
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3147)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3147)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980162.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 03:39:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,484
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 4,347
            CDSs (with protein)               :: 4,347
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 43 of 85
            Pseudo Genes (incomplete)         :: 37 of 85
            Pseudo Genes (internal stop)      :: 19 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3147
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039579-rid11723173.denovo.239"
                     /strain="LGE2415"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8302 N 95.9628 W"
                     /collection_date="2015-08-24"
     gene            27..518
                     /locus_tag="J1Y83_004220"
     CDS             27..518
                     /locus_tag="J1Y83_004220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000516891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBA7632299.1"
                     /translation="MGKKTAPLLPYTNQMLSEFGERLKLARLRRRLTAKQVAERAGMS
                     PITLRSLESGSAGVTIGAYLSVMQVLGLETDLNKLAAEDDLGRQLQDSRLIKKRGLSR
                     SSGKNEEGIQTDPFQTRYEKEGLLTTDLTALAEGSTPSITGETSTSLAALLKPIKKPL
                     KGN"
     gene            521..1789
                     /locus_tag="J1Y83_004221"
     CDS             521..1789
                     /locus_tag="J1Y83_004221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001606689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HipA domain-containing protein"
                     /protein_id="HBA7632300.1"
                     /translation="MALIGVYADWEGLDGPERIGYLHSRRTRTREIFEFEYDKKALAD
                     PSLNFIQLDPEIMLYEGAQYPIPPKDKFGAFSDSCPDRWGRMLMKRRFERDIRDGLCD
                     KDSHLYESDYLLGVHDLYRVGALRYKREDAGEFLDNRIDVAAPPFTEIASLERASRAI
                     EEDPDNKELMGQKWLRMLIAPGGSLGGARPKASVVDEAGHLYIAKFPSVKDEYDVGGW
                     EMVVNALAVGCGLNVAPAQAHKFASNYHCFMVRRFDRTNAGRRLHFASAMTLTRHQDG
                     EDASTGVSYLELADVLIRHGAQTNTDLKELWSRIVFNILVSNTDDHLRNHGYILIPGK
                     GWRLSEAYDLNPVARSDGLKLNITENDNALDLELAREVAEYFRLGLTEADDIIANFRG
                     IVSQWRIIAERLRLPGREQELMAEAFRGAI"
     gene            complement(1932..3101)
                     /locus_tag="J1Y83_004222"
     CDS             complement(1932..3101)
                     /locus_tag="J1Y83_004222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001555767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBA7632301.1"
                     /translation="METTGITKEQYSWIVSAFQLAYTLGQPIMGFFIDTVGLKLSFAI
                     CAAIWGLATMGHALTGTWSGLAFMRALMGFSEASAIPAGVKTASTWFPAKERGVATGV
                     FNMGTSLGAMLAPPLIAWCIMFHSWQFAFIVSGSLALLAALFWFFCYKDPKDAKRLSD
                     EERHYIESGQEQHLKTDKKEKTSIKHILSQRNFWGIGIARFLADPAWGTINFWVPIFF
                     VETLHFSLKEIAMFVWLPFLLGDLGCLASGFVAKFFHDRGVSLINSRRITFTIAAVIM
                     MTIGLVSIVENPYIAVLLISIGAFSHQCLSTVAATLGGDLFKKDEVATAVGMAGACAW
                     SGQLIFNLFIGAFVHIIGFAPFFIALAFFDIIGAIALWMLIKVKDEEPQVQLATS"
BASE COUNT          906 a          647 c          760 g          834 t
ORIGIN      
        1 ccataaaaag ataaaatatt atcactatgg gtaaaaaaac agcaccgtta ctcccttata
       61 cgaatcagat gcttagtgaa tttggtgagc ggctgaaact tgctcgttta cgacgcaggc
      121 ttaccgcaaa gcaagttgct gaacgtgctg ggatgtcgcc aataacattg cgctctctgg
      181 agtcaggaag tgcaggggtc acgatagggg catatttgtc tgtgatgcag gttttgggcc
      241 tggaaacgga tttaaataag cttgcagctg aagacgactt aggacgtcag ctccaggatt
      301 cccgtttgat taaaaaaaga gggctttctc gttcatcagg taaaaatgaa gaaggtattc
      361 agacagaccc ttttcaaacg aggtatgaga aagaaggttt actcaccacg gatttaacag
      421 cactggcaga aggctcaacg ccatctatta caggagaaac atcaacctct cttgcagcac
      481 tattaaaacc gataaaaaaa ccgttaaaag gaaattaatt atggcattaa tcggagtgta
      541 tgcggattgg gaaggcctgg atggtccgga gcggattggt tatttacaca gtcgccgcac
      601 tcggacgcgt gagatatttg agtttgagta cgataaaaag gcgcttgcag atccttcact
      661 taatttcatt cagttagatc ctgaaataat gttgtatgag ggagcacagt acccaattcc
      721 tccaaaggat aaatttggtg catttagtga ttcctgcccg gatcgttggg ggcgtatgct
      781 aatgaagcgt cgttttgaga gggatattcg agacgggtta tgtgataaag actcccacct
      841 ttatgagtct gattatctac taggtgttca tgatctttat cgggttgggg ctcttcgata
      901 caaacgtgaa gacgctggag agtttcttga taatcgcatt gatgttgcag ccccaccatt
      961 taccgaaata gcgagtctgg aaagggctag ccgggcaatt gaagaagatc ctgataataa
     1021 agagctaatg gggcaaaaat ggttaagaat gctaatcgct ccggggggat cattaggggg
     1081 ggcacgtccg aaagccagtg ttgttgatga agctgggcat ctctacatcg ctaaatttcc
     1141 cagtgtaaaa gatgagtatg atgttggtgg atgggaaatg gtcgttaatg cacttgctgt
     1201 tggctgtgga ctgaatgttg cacctgctca ggcgcataaa tttgccagca attaccattg
     1261 ttttatggtt cgtcgttttg accgcactaa tgcgggaagg cgtctgcatt ttgcttcggc
     1321 tatgacacta accaggcatc aggatggtga agatgcctct acaggtgtga gttatctgga
     1381 attagcagat gttcttatca ggcatggcgc gcagactaat actgatctta aggaactctg
     1441 gtccaggatt gtttttaata ttcttgtttc taataccgat gaccatttac gtaatcatgg
     1501 atatatcttg ataccgggaa aaggttggcg gctttctgag gcatatgatt tgaatcctgt
     1561 tgccaggtct gacggtctaa aactcaatat tacagaaaat gataatgcac ttgatttgga
     1621 attagctcga gaagttgcgg agtattttcg acttggtttg acagaagcag atgacattat
     1681 tgctaatttt agagggattg tcagtcagtg gcgaatcatt gccgagcgtc ttcgattacc
     1741 tggtcgtgag caagagttaa tggcagaggc gttcaggggg gcaatttaga atagttattt
     1801 tgatatattc gctaaatcat aaatttgcga agcaatgcca gtagatactg aacgttttgg
     1861 aagagacgtt taatttatga gaaaaataga aacgctacaa agctaaatta tagcgtttct
     1921 atgtaaaata attagcttgt cgctaactgt acttgtggtt cttcgtcttt aactttgata
     1981 agcatccaca gcgcaatggc gccaataatg tcaaagaaag ccagggcaat aaagaacggc
     2041 gcgaagccaa taatgtgaac gaatgccccg ataaacaggt tgaaaatcaa ctgaccgctc
     2101 catgcacagg ctcctgccat acccacagcg gtagctactt cgtctttttt gaataggtca
     2161 ccgcccagag ttgctgcaac agtagaaaga cattgatgcg agaatgcgcc aatgctaatc
     2221 agtaatacgg cgatgtaggg attttcgaca atactcacca gaccaatcgt catcataatg
     2281 acggctgcaa tagtgaaggt aattcttcgt gagttaatta aactcacgcc gcgatcgtgg
     2341 aagaacttcg cgacaaaacc actggctaaa cagccgagat cgcccagcag gaaaggcagc
     2401 cagacgaaca tggcaatttc tttcaggcta aaatgcagcg tttcgacgaa gaaaatcggt
     2461 acccagaagt taatggttcc ccatgccggg tcagcgagaa aacgcgcgat gccaatcccc
     2521 cagaaattac gttggctgag gatatgcttg attgacgttt tttctttctt atctgtttta
     2581 agatgctgtt cttgcccaga ttcaatatag tggcgctctt catcagaaag gcgtttagca
     2641 tcttttggat ctttataaca aaagaaccaa aataaagccg cgagtaaagc aaggctacct
     2701 gagacaataa acgcaaattg ccagctatga aacataatgc accaggcaat caacggtgga
     2761 gcaagcatcg cgccgagtga ggtgcccata ttgaaaacac ctgtcgccac gccacgctct
     2821 tttgccggga accatgttga tgcggttttt acacccgcag gaattgccga tgcttcgctg
     2881 aaacccatca gggcgcgcat aaatgccaga cctgaccatg ttcctgtcaa ggcatgcccc
     2941 attgtcgcca ggccccagat tgcggcacat atcgcaaaac ttaacttcag acccacggta
     3001 tcaataaaga agcccattat cggttgcccc aatgtatagg ccaactggaa tgcactgaca
     3061 atccatgaat attgttcttt ggttattcct gttgtctcca taatcgttgg agccgcaata
     3121 cctaacgccg ttctgtccag ataattg
//
LOCUS       DADSYM010000238         3112 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2415
            SAMN08039579-rid11723173.denovo.240, whole genome shotgun sequence.
ACCESSION   DADSYM010000238 DADSYM010000000
VERSION     DADSYM010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039579
            Sequence Read Archive: SRR6362533
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3112)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3112)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980162.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 03:39:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,484
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 4,347
            CDSs (with protein)               :: 4,347
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 43 of 85
            Pseudo Genes (incomplete)         :: 37 of 85
            Pseudo Genes (internal stop)      :: 19 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3112
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039579-rid11723173.denovo.240"
                     /strain="LGE2415"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8302 N 95.9628 W"
                     /collection_date="2015-08-24"
     gene            complement(18..425)
                     /gene="cesD2"
                     /locus_tag="J1Y83_004223"
     CDS             complement(18..425)
                     /gene="cesD2"
                     /locus_tag="J1Y83_004223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE chaperone CesD2"
                     /protein_id="HBA7632302.1"
                     /translation="MVDTFNDEVFNYYLEQKGYTIQKEFLCGSAFFIGWRIETPFFSL
                     AYRLDEQELILCSFEARNQTGLNGPVLSLTRLLEELYHHFSGIKKISAMKSKIGSDSE
                     RQKREELFNYFIRKGAVQQETEDGIWFVMNVNS"
     gene            complement(432..1397)
                     /gene="espB"
                     /locus_tag="J1Y83_004224"
     CDS             complement(432..1397)
                     /gene="espB"
                     /locus_tag="J1Y83_004224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001091991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE translocon
                     pore-forming subunit EspB"
                     /protein_id="HBA7632303.1"
                     /translation="MNTIDNNNAAIAVNSVLSSTTDSTSSTTTSTSSISSSLLTDGRV
                     DISKLLLEVQKLLREMVTTLQDYLQKQLAQSYDIQKAVFESQNKAIDEKKAGATAALI
                     GGAISSVLGILGSFAAINSATKGASDVAQQAASTSAKSIGTVSEASTKALAKASEGIA
                     DAADDAAGAMQQTIATAAKAASRTSGITDDVATSAQKASQVAEEAADAAQELAQKAGL
                     LSRFTAAAGRISGSTPFIVVTSLAEGTKTLPTTISESVKSNHDINEQRAKSVENLQAS
                     NLDTYKQDVRRAQDDISSRLRDMTTTARDLTDLINRMGQAARLAG"
     gene            complement(1418..2557)
                     /locus_tag="J1Y83_004225"
     CDS             complement(1418..2557)
                     /locus_tag="J1Y83_004225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_157140390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretion protein EspD"
                     /protein_id="HBA7632304.1"
                     /translation="MLETKSDILSTTSGANSTTFISDITQSERTSTLDFQLVKSSVAS
                     VSKADTGILPVPPAGHALVTPSAAEDILSKLFGGISGDVISRTGETESQSSTRSTTGY
                     PYLSQVNNVDPQAMMMMATLLSLDASAQRVASMKNSNEIYADGQNKALDNKTLEFKKQ
                     LEEQQKAEEKAQKSKIVGQVFGWLGVAATAIAAIFNPALWAVVAVSATAMALQTAVDV
                     MGDDAPQALKTAAQAFGGLSLAAGILTAGIGGVSSLISKVGDVANKVGSNIVKVVTTL
                     ADTFVENVASKISAVANGLTTSSRSIGTTVLNNDAAYYNVLSQVSAFAVENLTRQSEY
                     LSQSAKAELEKATLELQNQANYIQSASQLMSDSARVNSRIVSGRV"
     gene            complement(2570..>3112)
                     /gene="espA"
                     /locus_tag="J1Y83_004226"
     CDS             complement(2570..>3112)
                     /gene="espA"
                     /locus_tag="J1Y83_004226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE translocon
                     filament protein EspA"
                     /protein_id="HBA7632305.1"
                     /translation="SASTSTSMTYDLGNMSKDEVVELFKKVGVFQAALIMFAYMYQAQ
                     SELSIATYADMNESSKESTEAQKMANLVDAKIAEVQSSSEKDKKVKLPDEVISYIQDS
                     RNGISVSSDIDITKELGAGDLQTVKAAISAKANNLTTTVNNNQLTLQQMSNTLNLLTN
                     ARSDMQSLQYRTIQAISFGK"
BASE COUNT          849 a          729 c          618 g          916 t
ORIGIN      
        1 taataaaata atttttttta actatttacg ttcattacga accaaattcc gtcttctgtt
       61 tcttgctgca cggcaccctt tctgatgaag taattaaaca actcttcgcg cttttgacgt
      121 tctgaatctg aaccaatctt agatttcatc gcactgattt tcttaatacc cgaaaagtgg
      181 tgatacaact cttcgagcaa acgagtcagt gataaaacag ggccgttaag ccctgtttgg
      241 ttacgtgctt caaaagagca caaaatcagt tcttgttcat ccagtctgta cgctaatgaa
      301 aaaaaaggtg tctcaatccg ccatccgata aaaaaggcac tgccacaaag aaactccttc
      361 tgtattgtgt accctttttg ttcaagatag taattaaaca cttcatcatt aaacgtatcg
      421 accatgatca attacccagc taagcgagcc gcttgcccca tgcgattaat aaggtcagta
      481 agatcacggg ctgtcgttgt catatcacgt agacggctag agatatcatc ctgcgctctg
      541 cgaacatcct gtttgtaggt atctaaattc gacgcctgaa gattttctac agatttagca
      601 cgctgctcat tgatatcatg attggacttg acagattcag atatcgttgt tggcaatgtt
      661 ttcgtacctt cagcaaggct ggtaacaaca ataaatggcg tagaaccgga aatccttccg
      721 gcagcagcag taaagcgact taataatcct gccttctgtg ctaattcttg agcagcatca
      781 gcagcctctt cagctacctg agaagctttc tgagccgaag tagcaacatc atcagtgata
      841 ccggatgtac gactggccgc ttttgcagct gtcgcgatag tttgctgcat tgcgccagct
      901 gcatcatctg ctgcatctgc aataccttcg gaagccttcg ccagtgcttt agttgaagct
      961 tcagagactg taccgataga cttcgcagaa gtagaagcgg cttgctgagc gacatcactc
     1021 gcgcctttag ttgcgctatt aatggcagca aaagagccta aaatccccag gacagatgag
     1081 atagcaccac caatcagagc ggctgtcgca ccggcttttt tctcatcaat agctttattt
     1141 tggctctcaa aaacggcttt ttggatgtca tagctttgcg ccaattgctt ctgaagataa
     1201 tcttgcaatg tggtgaccat ctcacgcaac agtttttgaa cctccagcag aagtttagaa
     1261 atatccactc tgccatcagt aagtaaagat gaactgattg acgaagttga tgtagttgtt
     1321 gaactggtgc tgtcagtcgt gctgctcaaa acagaattaa ctgcgattgc cgcattgtta
     1381 ttatcgatag tattcataat aaacgcctct ttaataatta aactcgaccg ctgacaatac
     1441 ggctattcac tcgcgcagaa tcagacatca gctgagaagc actctgtata taattcgcct
     1501 ggttttgcag ctcgagagta gctttttcca gctctgcctt cgcactctga cttaagtact
     1561 cactttgtcg agttaaattt tccacagcaa aagcagaaac ctgtgataac acattataat
     1621 acgctgcgtc attattcagc acagttgtac caatagagcg cgaagaagta gttaaaccat
     1681 ttgccactgc cgaaattttc gatgcaacat tttcaacaaa agtgtcggcc agcgtagtga
     1741 ctacttttac gatgtttgaa ccaactttgt tagcgacatc gccaacttta gatataagtg
     1801 aagacacccc gccaatgccg gctgtcagaa tacctgcggc aagagataga ccgccaaaag
     1861 cttgcgctgc tgtttttaac gcctgtggag catcatcgcc cataacatca actgcagttt
     1921 gcagtgccat tgctgttgca ctaacagcaa caacagccca gagtgccggg ttaaaaatag
     1981 ctgcaatagc agttgctgca acccctaacc aaccaaagac ctgaccaaca attttacttt
     2041 tttgtgcttt ctcttcggct ttttgttgtt cttcgagctg ctttttaaac tccagcgttt
     2101 tattatccag cgctttattt tgtccatccg cgtagatctc gttggagttt ttcatactcg
     2161 caactctctg cgctgaggca tccagggata acagagtggc catcatcatc atggcctgag
     2221 ggtcaacgtt attcacctga gaaagataag gatagccagt tgtacttcgt gtactactct
     2281 gtgattctgt ctcaccagtg cgactaataa catcaccact gatgccgcca aaaagtttgc
     2341 tcagaatatc ttcagcagca gatggggtta ctaatgcatg acccgccggg ggtaccggta
     2401 aaatgccagt atcagcctta cttactgacg caacgctgga tttaaccagt tgaaaatcca
     2461 gcgttgaagt ccgctcagat tgagtgatat ctgaaataaa cgtagtgcta ttagcccctg
     2521 aggttgtaga caggatatcg ctttttgtct caagcatggt tatctcctgt tatttaccaa
     2581 aacttattgc ctgaatagtt cgatattgca acgactgcat atctgaacga gcatttgtta
     2641 ataaattcag cgtattagac atttgctgca acgtaagctg gttattattc accgtcgttg
     2701 tcaggttatt tgcttttgct gaaatagcag cttttacggt ttgcaggtca ccagcaccca
     2761 actctttggt gatgtcaata tcactactta cggaaatccc atttcgtgaa tcttgaatat
     2821 aactaattac ttcgtcagga agtttgacct ttttatcctt ttccgaacta gactgaactt
     2881 cagcgatctt ggcatccacc aaattggcca ttttttgtgc ctcggtggat tccttagatg
     2941 actcattcat gtctgcatat gtagcaatag atagctcgct ttgtgcctgg tacatataag
     3001 caaacatgat aagcgcagcc tgaaatacgc caactttttt aaacagctca acaacttcat
     3061 ctttcgacat gttccctaaa tcataggtca tcgatgtcga agtactcgca ct
//
LOCUS       DADSYM010000239         3109 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2415
            SAMN08039579-rid11723173.denovo.241, whole genome shotgun sequence.
ACCESSION   DADSYM010000239 DADSYM010000000
VERSION     DADSYM010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039579
            Sequence Read Archive: SRR6362533
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3109)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3109)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980162.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 03:39:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,484
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 4,347
            CDSs (with protein)               :: 4,347
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 43 of 85
            Pseudo Genes (incomplete)         :: 37 of 85
            Pseudo Genes (internal stop)      :: 19 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3109
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039579-rid11723173.denovo.241"
                     /strain="LGE2415"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8302 N 95.9628 W"
                     /collection_date="2015-08-24"
     gene            <1..1524
                     /locus_tag="J1Y83_004227"
     CDS             <1..1524
                     /locus_tag="J1Y83_004227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host specificity protein J"
                     /protein_id="HBA7632306.1"
                     /translation="AEVTADSGEYQVLARWDTPKVVKGVSFMLRLTVAADDGSERLVS
                     TARTTETTYRFRQLALGRYMLTVRAVNAWGQQGDPASVSFRIAAPAAPSRIELTPGYF
                     QITATPHLAVYDPTVQFEFWFSEKRITDIRQVETTARYLGTALYWIAASINIKPGHDY
                     YFYIRSVNTVGKSAFVEAVGRASDDAEGYLDFFKGKITESHLGKELLEKVELTEDNAS
                     RLEEFSKEWKDASDKWNAMWAVKIEQTKDGKHYVAGIGLSMEDTEEGKLSQFLVAANR
                     IAFIDPANGNETPMFVAQGNQIFMNDVFLKRLTAPTITSGGSPPVFSLTSDGKLTAKN
                     ADISGSVNANSGTLNNVTINENCQIKGKLSANQIEGDIVKTVSKSFPRTNSYASGTIT
                     VRISDDQKFDRQVMIPPVLFRGGKHENFNSNNQQSYWYSTCRLRVTRNGQEIFNQSTT
                     DAQGVFSSVIDMPAGQGTLTLTFTVSSSGANNWTPTTSISDLLVVVMKKSTAGISIS"
     gene            1591..2190
                     /locus_tag="J1Y83_004228"
     CDS             1591..2190
                     /locus_tag="J1Y83_004228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ail/Lom family outer membrane beta-barrel
                     protein"
                     /protein_id="HBA7632307.1"
                     /translation="MRKLYAAILSAAICLAVSGAPAWASEHQSTLSAGYLHVSTNVPG
                     SDDLNGINVKYRYEFTDTLGMVTSFSYAGDKNRQLTRYSDTRWHEDSVRNRWFSVMAG
                     PSVRVNEWFSAYAMAGVAYSRVSTFSGDYLRVTDNKGKTHDVLTGSDDARHSNTSLAW
                     GAGVQFNPTESVAVDVAYEGSGSGDWRTDGFIVGVGYKF"
     gene            2255..>3109
                     /locus_tag="J1Y83_004229"
     CDS             2255..>3109
                     /locus_tag="J1Y83_004229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415890.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA7632308.1"
                     /translation="MAVKISGVLKDGTGKPVQNCTIQLKAKRNSTTVVVNTLASENPD
                     EAGRYSMDVEYGQYSVILLVEGFPPSHAGTITVYEDSRPGTLNDFLGAMTEDDARPEA
                     LRRFELMVEEVARNASAVAQNTAAAKKSAGDAGTSASEAATHATDAAGSARAASTSAG
                     QAASSAQSASSSAGTASTKATEAEKSAAAAESSKSAAATSAGAAKTSETNAAASQQSA
                     ATSASTATTKASEAATSARDAAASKEAAKSSETNASSSASSSASSATAAANSAKAAKT
                     SETNARSSE"
BASE COUNT          769 a          782 c          922 g          636 t
ORIGIN      
        1 gcagaagtca ccgcagacag cggggaatat caggtgctgg cgcgctggga cacgccgaag
       61 gtggtgaagg gggtgagttt tatgcttcgc ctgaccgtgg ccgcggatga cggcagtgag
      121 cggctggtca gtacggccag gacgacggaa accacatacc gcttcaggca gctggcgctg
      181 gggcgttaca tgttgacggt ccgggcggta aatgcgtggg gacagcaggg cgatccggcg
      241 tcggtatcgt tccggattgc cgcaccggca gcgccgtcgc ggattgagct gacgccgggc
      301 tattttcaga tcaccgccac gccgcatctt gcggtttatg atccgacggt acagtttgag
      361 ttctggttct cggaaaagcg gattaccgat atcaggcagg ttgaaaccac agcccgctat
      421 cttggtacgg cgctgtactg gatagccgcc agtatcaata tcaaaccggg ccatgattat
      481 tacttttata tccgcagtgt gaacaccgtt ggcaaatcgg cattcgtgga ggccgtcggt
      541 cgggcgagcg atgatgcgga aggttacctg gattttttca aaggcaagat aaccgaatcc
      601 catctcggca aggagctgct ggaaaaagtc gagctgacgg aggataacgc cagcagactg
      661 gaggagtttt cgaaagagtg gaaggacgcc agtgataagt ggaatgccat gtgggctgtc
      721 aaaattgagc agaccaaaga cggcaaacat tatgtcgcgg gtattggcct cagtatggag
      781 gacacggagg aaggcaaact gagccagttt ctggttgctg ctaaccgtat cgcgtttatt
      841 gacccggcaa acgggaatga aacgccgatg tttgtggcgc agggcaacca gatattcatg
      901 aacgacgtgt tcctgaaacg cctgacggca cccaccatta ccagcggtgg cagtcctccg
      961 gtattttccc tgacatcaga cggaaagctg actgctaaaa atgcggatat cagtggcagt
     1021 gtgaatgcga actccgggac gctcaacaac gtcacgatta atgagaactg tcagattaag
     1081 gggaaactgt cagccaacca gattgaaggc gatattgtca aaacggtcag caagtctttc
     1141 ccccgcacga acagttatgc cagtggcacc atcacggtaa gaatcagtga tgatcagaaa
     1201 tttgaccggc aggtcatgat accgccagtg ttattccgcg gtggtaagca tgagaatttc
     1261 aacagtaata accaacagtc atactggtat tcaacctgcc ggttaagagt gacccgcaat
     1321 ggtcaggaga tttttaatca gtccacgacg gatgctcagg gcgtattttc ctcagttata
     1381 gatatgcctg ccggacaggg gacgctgaca ctgacattca ccgtatcttc atcaggagcg
     1441 aataactgga caccaacaac cagtatcagc gatctgctgg ttgtggtgat gaaaaaatcc
     1501 acagcaggta tcagtatcag ctgaatttta taacccagaa cgggcgtcag aaatgacgcc
     1561 ttttttattg cagaaaagcg agaggtaatt atgcgtaaac tttatgcagc cattttgtcc
     1621 gcagccattt gtctggccgt atccggtgcg cctgcatggg cgtctgaaca tcagtccacg
     1681 ctgagcgcgg ggtatcttca tgtctcgacg aacgttcccg gcagcgatga tctgaacggg
     1741 attaacgtga aataccgtta tgagtttacg gacacactgg ggatggtgac gtcattcagc
     1801 tatgcaggag acaagaatcg ccagcttacc cgttacagcg atacccgctg gcatgaagat
     1861 tccgttcgta accgctggtt cagcgtgatg gcggggccgt ctgtgcgcgt gaatgaatgg
     1921 ttcagcgcgt atgcgatggc gggtgtggct tacagccgtg tgtcgacttt ctccggggat
     1981 tatctccgcg taactgacaa caaggggaaa acgcacgacg tgctgaccgg aagtgatgac
     2041 gctcgccaca gtaacacctc tctggcgtgg ggagctggcg tgcagtttaa cccgaccgaa
     2101 tccgtggccg ttgatgtcgc ttatgaaggc tccggcagtg gcgactggcg cactgacggg
     2161 ttcatcgttg gtgttggtta taaattctga ttagccaggt aacacagtgt tatgacagcc
     2221 cgccggttca ggcgggcttt tttgtggggt gaatatggca gtaaagattt caggtgtact
     2281 gaaagacggc acaggaaaac cggtacagaa ctgcacaatc cagctgaaag caaaacgtaa
     2341 cagcaccacg gtggtggtga acacgctggc ctcagaaaat ccggatgaag ccgggcgtta
     2401 cagcatggac gttgagtacg gtcagtacag cgttattctg ttggtggagg gattcccgcc
     2461 gtcacatgcc gggactatca ccgtgtatga agattcccga cccggtacgc tgaatgattt
     2521 tctcggtgcc atgacggagg atgatgcccg tccggaggct ctgcgccgtt ttgaactgat
     2581 ggtggaagag gtggcgcgta acgcgtccgc ggtggcacag aacacggcag ccgcgaagaa
     2641 gtcagccggc gatgccggca catctgccag tgaggcggca acccatgcga ctgatgctgc
     2701 aggctcagca cgcgcagcca gcacgtcagc cggacaggcc gcttcgtcgg ctcagtcagc
     2761 gtcctccagc gcaggaacgg catcaacaaa ggccactgaa gcggaaaaaa gtgctgccgc
     2821 tgcagagtcc tcaaaaagcg cggcggctac cagtgccggt gcggcgaaaa cgtcagaaac
     2881 gaatgccgca gcgtcacaac aatcagcagc cacttctgca tccaccgcga ctacgaaagc
     2941 gtcagaagct gccacctcag cccgggatgc ggcggcctca aaagaggcag cgaaatcatc
     3001 agaaacgaac gcatcctcga gcgccagtag ttccgcttcc tcggcaacgg cggcagcaaa
     3061 ttccgcgaag gcggcaaaaa cgtccgagac gaacgccagg tcttctgaa
//
LOCUS       DADSYM010000240         2877 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2415
            SAMN08039579-rid11723173.denovo.242, whole genome shotgun sequence.
ACCESSION   DADSYM010000240 DADSYM010000000
VERSION     DADSYM010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039579
            Sequence Read Archive: SRR6362533
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2877)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2877)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980162.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 03:39:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,484
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 4,347
            CDSs (with protein)               :: 4,347
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 43 of 85
            Pseudo Genes (incomplete)         :: 37 of 85
            Pseudo Genes (internal stop)      :: 19 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2877
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039579-rid11723173.denovo.242"
                     /strain="LGE2415"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8302 N 95.9628 W"
                     /collection_date="2015-08-24"
     gene            complement(124..2826)
                     /gene="chiA"
                     /locus_tag="J1Y83_004230"
     CDS             complement(124..2826)
                     /gene="chiA"
                     /locus_tag="J1Y83_004230"
                     /EC_number="3.2.1.14"
                     /EC_number="3.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional chitinase/lysozyme"
                     /protein_id="HBA7632309.1"
                     /translation="MKLNIFTKSMIGMGLVCSALPALAMEAWNNQQGGNKYQVIFDGK
                     IYENAWWVSSTNCPGKAKANDATNPWRLKRTATAAEISQFGNTLSCEKSGSSSSSNSN
                     TPASNTPANGGSATPAQGTVPSNSSVVAWNKQQGGQTWYVVFNGAVYKNAWWVASSNC
                     PGDAKGNDASNPWRYVRAATATEISETSNPQSCTSAPQPAPDVKPAPDVKPAPDVQPA
                     PADKSNDNYAVVAWKGQEGSSTWYVIYNGSIYKNAWWVGAANCPGDAKENDASNPWRY
                     VRAATATEITQYGNPGSCSVKPDNNGGAVTPVDPTPETPETPVTPTPDNNEPSTPADS
                     SNDYSLQAWSGQEGSEIYHVIFNGNVYQNAWWVGSEDCPRGTSVENSNNPWRLVRTAT
                     AAEMSQYGNPTTCEIDNGGVIIADGFQASKAYSASSIVDYNDAHYKTSVDQDAWGFVP
                     GGDNPWKKYEPAKAWSASTVYVKGDRVVVDGQAYEALFWTQSDNPALIANQNATGSNS
                     RPWKPLGKTQSYSNEELNNAPQFNPETLYASDTLIRFNGENYISQSKVQKVSPSDSNP
                     WRVFVDWTGTKERVGTPKKAWPKHVYAPYVDFTLNTIPDLAALAKNHNVNHFTLAFVV
                     SKDANTCLPTWGTAYGMQNYAQYSKIKALREAGGDVMLSIGGANNAPLAASCKNVDDL
                     MQHYYDIVDNLNLRVLDFDIEGTWVADQASIERRNLAVKKVQDKWKSEGKDIAIWYTL
                     PILPTGLTPEGMNVLSDAKAKGVELAGVNVMTMDYGNAICQSANTEGQNIHGKCATSA
                     IANLHAQLKGLYGNKSDAEIDAMMGTTPMVGVNDVQGEVFYLSDARLVMQDAQKRNLG
                     MVGIWSIARDLPGGTNLSPEFHGLTKEQAPKYAFSEIFAPFTKQ"
BASE COUNT          704 a          745 c          666 g          762 t
ORIGIN      
        1 tatttcaatt acagaaagac tcttttttaa gcaaagaata gaaatatgaa taaaaataat
       61 gggctaccaa aagtagccca ttgacaaaaa atgcggcgat accggaaggt atcgccaaca
      121 catttattgc ttagtaaacg gcgcgaagat ttcgctaaat gcgtacttcg gagcctgttc
      181 tttagtcaga ccgtggaatt ccggagacag gttagtgccg cccggcaggt cgcgcgcgat
      241 tgaccagata ccaaccatac cgagattacg cttctgcgca tcctgcatga ccagacgagc
      301 atcagagaga tagaacacct caccctgaac gtcgttcacg ccaaccatcg gcgtggtacc
      361 catcatggcg tcaatttctg catcactctt attgccatag aggcctttca gctgcgcatg
      421 caggttggca atcgcagacg ttgcacactt accgtggatg ttctggcctt cggtgtttgc
      481 agactgacaa atagcgttac cgtagtccat tgtcatcacg ttcacacccg ccagctcaac
      541 acctttcgct ttggcatcgc tcaggacatt catcccttcc ggcgtcagac ctgtcggcag
      601 aattggcaag gtataccaga tagcaatatc tttgccttct gacttccatt tatcctgcac
      661 tttcttcaca gcaaggttac gacgttcaat agatgcctga tccgcaaccc aggtgccttc
      721 gatatcgaag tccaggactc tgaggttcag gttatcaacg atgtcataat aatgctgcat
      781 tagatcgtca acgttcttac aggaagctgc cagcggggca ttattagcac caccgataga
      841 cagcatcaca tcaccgccag cctcacgcag agctttgatt ttgctgtact gagcgtaatt
      901 ctgcataccg taagcggtac cccatgtcgg tagacaggtg ttcgcatctt tactcaccac
      961 aaacgccagc gtgaagtggt tgacgttatg attcttagcc agcgcagcca aatccgggat
     1021 cgtattcagc gtaaagtcga catacggtgc ataaacgtgt ttcggccacg ctttcttcgg
     1081 cgtacctacg cgctctttgg ttccggtcca gtcaacaaaa acacgccacg ggttgctgtc
     1141 agaaggagaa actttctgca ctttactctg agaaatgtag ttctcaccgt taaagcgaat
     1201 cagcgtatca ctggcataaa gcgtttctgg attaaactgc ggcgcattat tcagctcttc
     1261 gttgctatag ctctgagtct tacctaacgg cttccacggg cgactattgc taccggtggc
     1321 gttttggttc gcaatcagag cagggttgtc actttgcgtc cagaacagcg cttcataagc
     1381 ctgcccatca acaacaacgc gatcaccttt cacgtacacg gtggaagcgg accatgcttt
     1441 cgccggttcg tatttcttcc acgggttatc gccgccgggg acaaagcccc atgcgtcttg
     1501 atcgacagag gttttataat gtgcatcgtt ataatctacg atgctgctcg cgctgtacgc
     1561 tttgctggcc tggaaaccat ccgcaataat gacgccgcca ttatcaattt cacaggtagt
     1621 cgggttaccg tactgactca tttccgcagc ggtagctgta cgcacgaggc gccatgggtt
     1681 attggagttt tctacactgg tgccacgcgg gcaatcttca gaaccaaccc accaggcatt
     1741 ctgataaaca ttaccattga agataacatg gtaaatttcg ctgccttcct ggccactcca
     1801 cgcctgcagt gagtaatcgt tactgctatc cgctggtgtt gatggctcgt tgttatccgg
     1861 agttggagta actggagttt ccggtgtttc cggagttgga tcaaccggag tcacagcacc
     1921 gccattatta tccggcttaa cggaacagga gcctgggtta ccatactgag tgatttctgt
     1981 tgccgttgcc gcgcgaacat aacgccatgg gttgctggca tcgttttctt tcgcatcgcc
     2041 tgggcaattt gccgcgccta cccaccaggc gttcttataa atgctgccgt tgtagataac
     2101 gtaccatgta gaagaacctt cctgaccttt ccaggctacg acagcatagt tgtcgtttga
     2161 cttatcagct ggggctggct gaacatcagg agccggttta acgtccggtg ccggtttcac
     2221 atccggtgca ggctgtggtg ctgaagtaca agactgtgga ttactggttt ctgagatttc
     2281 cgttgccgta gcggcacgaa cataacgcca tgggttgctg gcatcattgc ctttcgcatc
     2341 acccgggcag ttagaagagg ctacccacca ggcattttta tataccgcac cattaaagac
     2401 gacataccag gtttgaccgc cctgctgttt attccaggca actacagaag aattagacgg
     2461 aacagtgccc tgtgctggtg tagccgaacc gccattagca ggcgtattag atgctggcgt
     2521 atttgaattt gaagaagatg agctgccgct cttttcgcag gaaagtgtat tgccaaactg
     2581 actaatttca gcagctgttg cggtacgctt taaacgccac gggttagttg catcatttgc
     2641 tttcgctttt cccgggcaat ttgtagaaga aacccaccag gcattttcat aaattttgcc
     2701 atcgaaaata acctgatatt tattaccacc ttgttggtta ttccatgctt ccattgccaa
     2761 tgctggcaga gcggaacaca ccagccccat accaatcata gatttagtaa atatatttaa
     2821 tttcattata atcccttgta acgtaaaaac tgcaaaacaa aagtcctacg caggaat
//
LOCUS       DADSYM010000241         2844 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2415
            SAMN08039579-rid11723173.denovo.243, whole genome shotgun sequence.
ACCESSION   DADSYM010000241 DADSYM010000000
VERSION     DADSYM010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039579
            Sequence Read Archive: SRR6362533
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2844)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2844)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980162.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 03:39:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,484
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 4,347
            CDSs (with protein)               :: 4,347
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 43 of 85
            Pseudo Genes (incomplete)         :: 37 of 85
            Pseudo Genes (internal stop)      :: 19 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2844
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039579-rid11723173.denovo.243"
                     /strain="LGE2415"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8302 N 95.9628 W"
                     /collection_date="2015-08-24"
     gene            complement(<1..358)
                     /locus_tag="J1Y83_004231"
     CDS             complement(<1..358)
                     /locus_tag="J1Y83_004231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000038144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA7632310.1"
                     /translation="MSKQKSKKNVIMAIGEACDQYALNNPRKYNYEHDAQPGEELIFI
                     LEEPSPELTDEELSIIQSMDGVYFNPRPRKTTIIDSSLLKRWQCLGFQDMNHFRGYMA
                     LNSLQYAGARETELLML"
     gene            complement(697..954)
                     /locus_tag="J1Y83_004232"
     CDS             complement(697..954)
                     /locus_tag="J1Y83_004232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000876742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA7632311.1"
                     /translation="MKYNLTLNKRTFEITESGYTLAKQLADSNYELPAIISFQELPHS
                     KEHTQHWLNHLSHFGVIQYEAVNNQTFRLLEISPFELWVTL"
     gene            complement(967..1632)
                     /locus_tag="J1Y83_004233"
     CDS             complement(967..1632)
                     /locus_tag="J1Y83_004233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001706515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBA7632312.1"
                     /translation="MNQNTKTIAEAKAQCLNKKDSKQTYVVVEQWLKMFKKWFSPSGK
                     EYTATLPAVEIYSYVAGFNEQGQECYANQKTLANIGMVSDRQVRNIINMFIDMGVLSV
                     EARDGQTSLYKALPFTDAHITPPEDVKTSVNVEQPAPETQEPLEATHSIEQETQAEIT
                     EQSDLFTWLISIDSQKPMQEETENFIKRALTSKRIILEDDAFNALMIALRKESEIYDS
                     IPF"
     gene            complement(1780..1962)
                     /locus_tag="J1Y83_004234"
     CDS             complement(1780..1962)
                     /locus_tag="J1Y83_004234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000870791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA7632313.1"
                     /translation="MKWYTPEHVVEAFKKGELTRHQVRMNRNTARRCGYPEREKCFDD
                     ALKIIDELRKAEAEKE"
BASE COUNT          739 a          577 c          514 g         1014 t
ORIGIN      
        1 caagcataag taactctgtc tctcttgcgc ctgcatactg caaactatta agagccatgt
       61 agccacgaaa atgattcata tcctggaagc ctaaacactg ccacctttta agaaggctgc
      121 tatcaatgat tgttgttttg cgtgggcgtg gattgaagta aacaccatcc atactttgaa
      181 tgattgataa ttcttcgtct gtcaattctg ggcttggttc ttcaagtata aagatcaatt
      241 cctcgcctgg ttgtgcatca tgctcataat tatatttgcg aggattgttt aacgcatact
      301 gatcacatgc ttcgccaata gccattatta cgtttttctt gcttttttgc ttgctcattg
      361 cttccccttc gttttggttt tcttgttgca gtgttaaaaa aggaagcgat catgaatact
      421 aagaaaaata ttaagctttc cattgtttca cccttcaaga tgtttgatag ttgtttgttg
      481 tttgttgttt gttttaagct acagagtcga ttctgaatgc tttcatatga gtttttgtat
      541 actgcaataa tccttgttta cttaggttat cgagaagagc atataaaaat tgctcaccta
      601 ccacaccgga aaactcagaa tcaaacagaa tcattcccgt tctttcccgt aaatattgca
      661 gaaagatttc ttgtgaaaat tcgccatcga atatgatcac agtgtcaccc acaattcaaa
      721 cgggcttatc tcaagcagtc tgaacgtttg attattcaca gcttcatact gaattacccc
      781 aaaatggctt aggtgattta gccaatgctg cgtgtgttct ttactgtgcg gtaactcctg
      841 aaagctgata atcgcgggta gctcataatt gctatcagca agctgttttg ccagtgtgta
      901 gccgctttct gtaatttcga atgttcgttt gtttaatgtc aggttgtatt tcatttttta
      961 ttctccttaa aatggtattg agtcatagat ttcactttct tttctcaggg ctatcatcag
     1021 tgcattaaaa gcatcatctt caagaattat cctcttcgag gtcagtgcac gttttataaa
     1081 attttctgtt tcttcttgca taggcttttg gctgtctatg gaaataagcc atgtaaaaag
     1141 atcagattgt tccgtgattt ctgcctgtgt ttcctgctca atagagtgtg tcgcttctaa
     1201 cggttcctgt gtttcaggtg caggctgttc tacgtttaca gaggttttta cgtcttccgg
     1261 tggtgtgatg tgtgcatctg taaagggtaa tgctttatag agtgatgttt gcccgtcccg
     1321 tgcctctaca gaaagaacac ccatatcaat aaacatattt ataatattac gcacctgacg
     1381 atcactaacc attccaatat tagctaatgt tttttgattg gcgtaacatt cttgcccttg
     1441 ttcattgaac ccagctacgt aactgtagat ttcaacagct ggaagtgtcg cggtgtattc
     1501 cttacctgac ggtgagaacc actttttaaa catcttcagc cattgttcaa ctactacata
     1561 tgtttgttta ctatctttct tattgaggca ttgcgctttt gcttcggcaa ttgttttggt
     1621 gttttggttc atttagttcc tcctttgtaa ggactgtaat tagtaattca ttaacaagtt
     1681 ttggaattga tttcccttct gcctttgcac gatttaacaa cgcgttataa gttgcagaat
     1741 caaaattaat attcatttgg ttttctcctt ctcctgttat tattctttct ctgcttctgc
     1801 cttacgcagt tcatcaataa tttttagtgc atcgtcaaag catttttcac gctctggata
     1861 accacagcgc cgcgctgtgt tcctgttcat ccttacttga tgtcgtgtta gctcaccttt
     1921 tttaaatgcc tctacgacgt gttccggtgt ataccatttc ataaaaaact ccttatttag
     1981 ataaaagaaa aacacacctt gcagcctgac ggctcaaagt gcagtgttct gtgatttgat
     2041 tatttagatg gagagagatg tttctcccct ccctacttac aatactacat aacggcggct
     2101 ttgtcaaccc tcttttataa aaaatttgat cattttcaat ttcttataat ttttcttagc
     2161 tttttgtgct gcctactctg gtacaattct tccctgttgt ctaaaacgct ctcaatcgca
     2221 ctctgacggg gttttgataa aaacaataca ttcccttatg tagaggttgt ttatctcgtc
     2281 agaggcttat ttaaggggct tatttttgat ttatcagatg atggaaagat ggtggataga
     2341 taaactctga tttcagacat cttataaaat acctgcaaag ttctcagaag ctctgtaacg
     2401 agctaatcta ttaccgctaa ggtgtttccc ttacattacc ctgtttagct cgcctacggc
     2461 ttgatttaag cggtagaatc ttatttaacc ttcaactaca aaaaccattc tttctttttt
     2521 gttgttggtt gttttgtcat gttatgcgtt agcatcccca aagggtaacg cgacactaac
     2581 aaccattcgc ttacgctcag gttattgcac aagtaaattt caatcgttag atggtagtta
     2641 gttaattatc gttctgcgca cagcgcaaga ctattagcca tacgcaagcc gtaggctatt
     2701 tgcacatagt agattacata ttcgttgtga gcggaaagcg aacatagatc agctaactac
     2761 taacaaaaaa caaaaaacaa aaactatctg ttatgacaag catcagtcta acttgtgcaa
     2821 ggttctggcg aatgccaata gatt
//
LOCUS       DADSYM010000242         2831 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2415
            SAMN08039579-rid11723173.denovo.244, whole genome shotgun sequence.
ACCESSION   DADSYM010000242 DADSYM010000000
VERSION     DADSYM010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039579
            Sequence Read Archive: SRR6362533
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2831)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2831)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980162.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 03:39:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,484
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 4,347
            CDSs (with protein)               :: 4,347
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 43 of 85
            Pseudo Genes (incomplete)         :: 37 of 85
            Pseudo Genes (internal stop)      :: 19 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2831
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039579-rid11723173.denovo.244"
                     /strain="LGE2415"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8302 N 95.9628 W"
                     /collection_date="2015-08-24"
     gene            78..1517
                     /gene="norV"
                     /locus_tag="J1Y83_004235"
     CDS             78..1517
                     /gene="norV"
                     /locus_tag="J1Y83_004235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic nitric oxide reductase
                     flavorubredoxin"
                     /protein_id="HBA7632314.1"
                     /translation="MSIVVKNNIHWVGQRDWEVRDFHGTEYKTLRGSSYNSYLIREEK
                     NVLIDTVDHKFSREFVQNLRNEIDLADIDYIIINHAEEDHAGALTELMAQIPDTPIYC
                     TANAIDSINGHHHHPEWNFNVVKTGDTLDIGNGKQLIFVETPMLHWPDSMMTYLTGDA
                     VLFSNDAFGQHYCDEHLFNDEVDQTELFEQCQRYYANILTPFSRLVTPKITEILGFNL
                     PVDMIATSHGVVWRDNPTQIVELYLKWAADYQEDRITIFYDTMSNNTRMMADAIAQGI
                     AETDPRVAVKIFNVARSDKNEILTNVFRSKGVLVGTSTMNNVMMPKIAGLVEEMTGLR
                     FRNKRASAFGSHGWSGGAVDRLSTRLQDAGFEMSLSLKAKWRPDQDALELCREHGREI
                     ARQWALAPLPQSTVNTVVEEETSAATTADLGPRMQCSVCQWIYDPAKGEPMQDVAPGT
                     PWSEVPDNFLCPECSLGKDVFDELASEAK"
     gene            1514..2647
                     /gene="norW"
                     /locus_tag="J1Y83_004236"
     CDS             1514..2647
                     /gene="norW"
                     /locus_tag="J1Y83_004236"
                     /EC_number="1.18.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavorubredoxin reductase NorW"
                     /protein_id="HBA7632315.1"
                     /translation="MSNGIVIIGSGFTARQLVKNIRKQDASIPLTLIAADSMDEYNKP
                     DLSHVISQGQRADDLTRQTAGEFAEQFNLRLFPHTWVTDIDAEAHVVKSQNNQWQYDK
                     LVLATGASAFVPPVPGRELMLTLNSQQEYRACETQLRDARRVLIIGGGLIGSELAMDF
                     CRAGKMVTLIDNAASILASLMPPEVSSRLQHRLTEMGVHLLLKSQLQGLEKTDSGILA
                     TLDRQRCIEVDAVIAATGLRPETALARRAGLTINRGVCVDSYLQTSNADIYALGDCAE
                     INGQVLPFLQPIQLSAMVLAKNLLGNNTPLKLPAMLVKIKTPELPLHLAGETQRQDLR
                     WQINTERQGMVARGVDDADQLRAFVVSEDRMKEAFGLLKTLSM"
BASE COUNT          713 a          722 c          783 g          613 t
ORIGIN      
        1 attagataaa aaactggcac gcaatctgca atcagcaaga catcttttta gaacacgctg
       61 aataaattga ggttgctatg tctattgtgg tgaaaaataa cattcattgg gttggtcaac
      121 gtgactggga agtgcgtgat tttcacggta cagaatataa aacgctgcgc ggcagcagct
      181 acaacagcta cctcatccgc gaagaaaaaa acgtgctgat cgacaccgtc gaccataaat
      241 tcagccgcga atttgtgcag aacctgcgta atgaaatcga tctggcggat atcgattaca
      301 tcataattaa ccatgctgaa gaggaccacg ccggggcgct gaccgaactg atggcacaaa
      361 ttcccgatac gccgatctac tgtactgcca acgctatcga ctcgataaat ggtcatcacc
      421 atcatccgga gtggaatttt aatgtagtga aaactggcga cacgctggat atcggcaacg
      481 gcaaacaact catttttgtc gaaacgccaa tgctgcactg gccggacagc atgatgactt
      541 acctgacagg cgacgcggtg ctgttcagta acgatgcttt cggtcagcac tactgcgacg
      601 agcatctgtt caacgatgaa gtggatcaga ctgagctttt cgagcagtgc cagcgttact
      661 acgccaatat cctgacgccg ttcagccgcc tggtaacgcc gaaaattacc gagatcctgg
      721 gctttaactt gccagtcgat atgatagcga cctctcacgg cgtggtatgg cgcgataacc
      781 cgacgcaaat tgtcgagctg tacctgaaat gggcggcgga ttatcaggaa gacagaatca
      841 ccattttcta cgacaccatg tcgaataata cccgcatgat ggccgatgct atcgcccagg
      901 gtattgcgga aaccgaccca cgcgtggcgg tgaaaatttt caacgtcgcc cgaagcgata
      961 aaaacgaaat cctgaccaat gtcttccgct caaaaggcgt gctggtcggc acttctacga
     1021 tgaataatgt gatgatgccg aaaatcgccg ggctggtgga ggagatgacc ggattacgct
     1081 tccgtaacaa acgcgccagc gctttcggct ctcacggctg gagcggcggt gcggtggatc
     1141 gtctttccac gcgcctgcag gatgcgggtt tcgaaatgtc gcttagcctg aaagccaaat
     1201 ggcgaccaga ccaggacgct ctggagttat gtcgtgaaca cggtcgcgaa atcgcccgtc
     1261 agtgggcgct cgcgccgctg ccgcagagca cggtgaatac ggtggttgaa gaagaaacct
     1321 ctgccgccac gacggctgac ctcggcccac ggatgcaatg cagcgtctgc cagtggattt
     1381 acgatccggc aaaaggcgag ccaatgcaag acgttgcgcc aggaacgccg tggagtgaag
     1441 tcccggataa cttcctgtgc ccggaatgct ctctcggcaa agatgtcttt gacgaactgg
     1501 catcggaggc aaaatgagca acggcattgt gatcattggt tcgggcttca ccgcccgcca
     1561 actggtgaaa aatattcgca aacaggacgc cagtattcca ttaaccctga ttgccgccga
     1621 cagcatggat gagtacaaca aacctgacct cagccatgtt atcagtcagg ggcaacgtgc
     1681 cgacgacctt acccgccaga cggcaggtga atttgccgag cagtttaatc tgcgcctgtt
     1741 tccgcacacc tgggtaacgg atatcgatgc cgaagcccat gtggtgaaaa gtcagaataa
     1801 tcagtggcaa tacgacaagt tagtgctggc aaccggtgcc agcgcctttg tcccgccagt
     1861 gcccgggcgt gagttaatgc tgacgttaaa tagtcagcaa gagtatcgcg cctgtgaaac
     1921 gcaactgcgg gatgcccgac gcgtgttgat tatcggcggt ggtttgattg gtagcgaact
     1981 ggcgatggat ttttgtcggg caggcaaaat ggtcacgcta atcgacaacg ctgccagtat
     2041 tctggcgtcg ttaatgccac cggaagtcag cagccgcttg cagcatcggt tgacggagat
     2101 gggcgttcat ctgctgttga aatctcagtt gcagggacta gaaaaaacag attctggcat
     2161 tctggcaacg ctggaccgcc agcgctgcat cgaagtggat gcggtaattg ccgccaccgg
     2221 actgcgcccg gaaaccgccc tggcacgacg cgccgggctg acgattaatc gcggcgtttg
     2281 cgtcgatagt tatctgcaaa ccagtaatgc cgatatttat gcgctgggcg attgcgcgga
     2341 aattaacggt caggtattgc cgttcctcca gccgattcaa cttagtgcaa tggtgctggc
     2401 aaaaaatctt ctcggcaata acacgccgct gaaactcccg gcgatgctgg tgaaaatcaa
     2461 aacgccggaa ttaccgctgc atctggcagg cgaaacccag cgtcaggatt tacgctggca
     2521 aattaatacc gaacgccagg gaatggtggc gcgcggtgtt gacgatgctg accagcttcg
     2581 cgcctttgtg gtcagtgagg atcggatgaa agaggcattt ggattgttga aaacgttgtc
     2641 gatgtaggcg agctacagtg ccccaaatgt cggatgcggc gctgacgcgt cttatccgac
     2701 ctacagggaa cacatgtgtt gatcacataa tacgtttatg tcgcatcctg cactgtccca
     2761 aaatgtcgga tgcgacgctg gcgcgtctta tccgacctgc ggggatgaat atgtaggccg
     2821 gataaggcgt t
//
LOCUS       DADSYM010000243         2774 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2415
            SAMN08039579-rid11723173.denovo.245, whole genome shotgun sequence.
ACCESSION   DADSYM010000243 DADSYM010000000
VERSION     DADSYM010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039579
            Sequence Read Archive: SRR6362533
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2774)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2774)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980162.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 03:39:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,484
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 4,347
            CDSs (with protein)               :: 4,347
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 43 of 85
            Pseudo Genes (incomplete)         :: 37 of 85
            Pseudo Genes (internal stop)      :: 19 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2774
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039579-rid11723173.denovo.245"
                     /strain="LGE2415"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8302 N 95.9628 W"
                     /collection_date="2015-08-24"
     gene            119..2398
                     /gene="ydeP"
                     /locus_tag="J1Y83_004237"
     CDS             119..2398
                     /gene="ydeP"
                     /locus_tag="J1Y83_004237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance putative oxidoreductase YdeP"
                     /protein_id="HBA7632316.1"
                     /translation="MKKKIESYQGAAGGWGAVKSVANAVRKQMDIRQDVIAMFDMNKP
                     EGFDCPGCAWPDPKHSASFDICENGAKAIAWEVTDKQVNASFFAENTVQSLLTWGDHE
                     LEAAGRLTQPLKYDDVSDCYKPLSWQQAFDEIGARLQSYSDPNQVEFYTSGRTSNEAA
                     FLYQLFAREYGSNNFPDCSNMCHEPTSVGLAASIGVGKGTVLLEDFEKCDLVICIGHN
                     PGTNHPRMLTSLRALVKRGAKMIAINPLQERGLERFTAPQNPFEMLTNSETQLASAYY
                     NVRIGGDMALLKGMMRLLIERDDAASAAGRPSLLDDEFIQTHTVGFDELRRDVLNSEW
                     KDIERISGLSQTQIAELADAYAAAERTIICYGMGITQHEHGTQNVQQLVNLLLMKGNI
                     GKPGAGICPLRGHSNVQGDRTVGITEKPSAEFLDRLCERYGFTPPHAPGHAAIASMQA
                     ICTGQARALICMGGNFALAMPDREASAVPLTQLDLAVHVATKLNRSHLLTARHSYILP
                     VLGRSEIDMQKSGAQAVTVEDSMSMIHASRGVLKPAGVMLKSECAVVAGIAQAALPQS
                     VVAWEYLVEDYDRIRNDIEAVLPEFADYNQRIRHPGGFHLINAAAERRWMTSSGKANF
                     ITSKGLLEDPSSAFNSKLVMATVRSHDQYNTTIYGMDDRYRGVFGQRDVVFMSAKQAK
                     ICRVKNGERVNLIALTPDGKRSSRRMDRLKVVIYPMADRSLVTYFPESNHMLTLDNHD
                     PLSGIPGYKSIPVELEPSN"
     gene            2646..>2774
                     /locus_tag="J1Y83_004238"
     CDS             2646..>2774
                     /locus_tag="J1Y83_004238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Two-protein-system connector protein SafA"
                     /protein_id="HBA7632317.1"
                     /translation="MHATIVKNKITQRDNYKGIMSVIVVVLLLTLTLIAIFSAIDQL"
BASE COUNT          749 a          646 c          670 g          709 t
ORIGIN      
        1 atagtaagag agtagtcccc tactcttata tattcatgtt gacgataaac cctttgtata
       61 tactttgcat catcgctatt acaaatccta ataattcatt tccacacagg ataagcagat
      121 gaagaaaaaa attgaatcct accagggagc tgccggtggt tggggtgctg ttaaatccgt
      181 agcgaatgca gtacgtaagc agatggatat acgccaggat gttattgcca tgtttgacat
      241 gaataagcca gagggctttg actgtccggg atgtgcatgg ccagatccta agcacagtgc
      301 gtcattcgac atttgtgaaa acggcgcaaa agcaatcgcc tgggaagtca cggataagca
      361 ggtaaatgcc tctttctttg ctgagaatac ggttcaatca ttacttacct ggggagatca
      421 cgagcttgag gctgcgggac gtctcactca gcctttgaaa tatgatgacg tcagcgactg
      481 ttacaagcca ttaagctggc aacaagcttt cgacgaaatt ggcgctcgcc ttcaaagcta
      541 tagtgatccc aatcaggttg aattctatac ttcggggcgc acttccaatg aagctgcctt
      601 tctttatcag ctttttgccc gtgaatacgg gagcaataac tttcccgact gctccaacat
      661 gtgccatgaa ccgaccagtg tgggattggc agcgagtatc ggtgtgggta aagggaccgt
      721 gttgctggaa gactttgaga aatgcgattt agtcatttgc attgggcata accctggtac
      781 aaaccaccct cgcatgctga cttcgttgcg cgctttagtg aaacggggag cgaaaatgat
      841 cgctatcaac cctctacagg aacgtggcct ggagcgattt accgcaccgc aaaacccgtt
      901 tgaaatgctg acgaactctg agactcagtt ggccagtgcc tactataacg tgcgcattgg
      961 tggcgatatg gcattgctca aggggatgat gcgcctgtta attgagcgcg atgatgctgc
     1021 aagcgccgca ggtcggccct cattgctgga tgacgaattt attcaaacgc ataccgtcgg
     1081 ctttgacgag ctacgccgtg acgtgctcaa ttccgagtgg aaagatatcg aacgtatttc
     1141 tggactaagt cagacacaaa tcgccgaact tgctgatgca tatgccgctg ccgaacgaac
     1201 tattatctgt tacggaatgg ggatcactca gcacgaacat ggtacccaga acgtacagca
     1261 actggtcaat ctgctgttaa tgaaaggtaa cattggcaag cctggtgcgg gtatctgccc
     1321 actacgaggt cactctaatg tacagggcga ccgaaccgtc ggtatcaccg agaaaccgtc
     1381 tgcagagttt ctggatcgcc tgtgtgagcg ctatggcttc accccacctc atgcacctgg
     1441 acatgctgca atcgccagca tgcaagctat atgtacgggg caagctcgag cattgatctg
     1501 catggggggc aactttgcac tggcaatgcc agatcgggaa gcgagcgctg taccgttaac
     1561 gcaattagat ctggcggtac acgtagccac taagcttaac cgctctcatc tgctgaccgc
     1621 acggcatagc tatattctgc cggttctggg acgtagcgag attgacatgc aaaaaagcgg
     1681 tgcgcaggcg gtaaccgttg aagattcaat gtcgatgatt catgcctcgc gtggtgtgtt
     1741 aaaacccgcc ggtgtaatgc tgaaatcaga gtgtgcagtg gtcgcgggaa tcgcgcaagc
     1801 agcactaccc cagagcgtgg tagcctggga gtatctggtg gaagattatg atcgcattcg
     1861 caatgacatt gaagctgtgc tgccagagtt tgccgactat aaccagcgca tccgtcatcc
     1921 tggtggtttt cacctgataa acgcagcagc tgaaaggcgc tggatgacgt cgtcaggtaa
     1981 ggctaatttc attaccagta aagggctgtt agaagatccc tcttcagcgt ttaacagtaa
     2041 gctggtcatg gcgacagtac gcagtcacga tcagtacaac acgacgattt atggtatgga
     2101 tgatcgctat cgaggggtat tcggtcaacg agatgtggtc tttatgagtg ctaaacaagc
     2161 taaaatttgc cgtgtaaaaa acggcgaaag agttaatctt attgcactca cgccagacgg
     2221 taaacgtagc tcacgccgca tggatagatt aaaagtggtc atttacccta tggctgaccg
     2281 ctcactggtg acctattttc cagaatcgaa tcacatgcta acacttgata accacgatcc
     2341 attaagtggc attcctggct ataaaagtat tccggttgaa ttagaaccat caaattaatt
     2401 cgcctcctca tttcttctgc tgtcatccgc acagcagaag aattcctcat tgactattat
     2461 cgcgcaattt gctcacatgg attaaattaa accacacact ataagatata aacttctgcc
     2521 tacagctgta agaaactccg ctcagtactg aagtacccgt cctatttcct ctattctcca
     2581 gcctgttata ttaagcatac tgattaacga tgcttaacgt cattcgttta ataaacacat
     2641 ttgaaatgca tgcgaccata gtgaaaaata aaatcacgca aagagacaac tataaaggga
     2701 tcatgtctgt aattgtggtt gtcttattac tgacccttac attgatagcc attttttcgg
     2761 caattgatca gctg
//
LOCUS       DADSYM010000244         2689 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2415
            SAMN08039579-rid11723173.denovo.246, whole genome shotgun sequence.
ACCESSION   DADSYM010000244 DADSYM010000000
VERSION     DADSYM010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039579
            Sequence Read Archive: SRR6362533
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2689)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2689)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980162.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 03:39:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,484
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 4,347
            CDSs (with protein)               :: 4,347
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 43 of 85
            Pseudo Genes (incomplete)         :: 37 of 85
            Pseudo Genes (internal stop)      :: 19 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2689
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039579-rid11723173.denovo.246"
                     /strain="LGE2415"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8302 N 95.9628 W"
                     /collection_date="2015-08-24"
     gene            32..757
                     /locus_tag="J1Y83_004239"
     CDS             32..757
                     /locus_tag="J1Y83_004239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005112197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adhesin"
                     /protein_id="HBA7632318.1"
                     /translation="MKIFNSSFIANISSTIRTHLPILQKNIRPHPSPNLDVTSHHRVF
                     QKDGIGLSQTGKSTQLVIMSHGGWKEISHPQTLFTRQKGDGWTVVPQNLRLDFYTKDN
                     DFTKGLSVLSEVNKRHTEAQTGLTLSLNISQDDLSFLANARNIPQSKLEEEMKSQAIY
                     RKEYAISNEKIKNYALYYHDQAKNIIQKHQDGSGNQNVDIAFITDKNHKKHLSDIFKV
                     INESGVKYDVIHFGACRVNREEQ"
     gene            complement(983..1306)
                     /locus_tag="J1Y83_004240"
     CDS             complement(983..1306)
                     /locus_tag="J1Y83_004240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nikA protein"
                     /protein_id="HBA7632319.1"
                     /translation="MTKRSGSNTRRRAISRPVRLTAEEDQEIRKRAAECGKTVSGFLR
                     AAALGKKVNSLTDDRVLKEVMRLGALQKKLFIDGKRVGDREYAEVLIAITEYHRALLS
                     RLMAD"
     gene            1590..1784
                     /locus_tag="J1Y83_004241"
     CDS             1590..1784
                     /locus_tag="J1Y83_004241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="HBA7632320.1"
                     /translation="MNKQQQAVLNMAGFIKSQSLTLLEKLDALDADEQAAMCEKLHEL
                     AEELQNSIQTRFEAENRTGI"
     gene            1973..2344
                     /locus_tag="J1Y83_004242"
     CDS             1973..2344
                     /locus_tag="J1Y83_004242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011898200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA7632321.1"
                     /translation="MSKKQSTGRSQRRRRFSIGSAPLTSITKIDAQARGGETRQDYKD
                     TRRFPPEAPSCALLFRPCRLPDTCPPFSLREAWRFLIAHAVGISARCRSFAPSWAVCT
                     NPPFSPTAAPYPVTIVLNPTR"
BASE COUNT          855 a          669 c          570 g          595 t
ORIGIN      
        1 aaaaaaaact gcaaaaaagg attgttaaca aatgaaaata ttcaattcaa gttttatagc
       61 aaacatatca tctacaataa gaacccacct cccgatacta caaaaaaata taagaccaca
      121 tccatcacca aacctcgacg taacaagtca tcatcgtgta ttccagaaag acggaatagg
      181 attgagccaa acaggaaaat caacacaact tgttattatg tcgcacggtg gatggaaaga
      241 gatctcccat ccacaaacac tattcacgcg tcaaaaaggc gatggatgga ctgttgtacc
      301 ccaaaaccta aggcttgact tttatactaa agataacgac ttcacaaagg gactaagcgt
      361 tctttcagag gtaaacaaac gccatactga agcacaaaca gggttaacac tatcactaaa
      421 cataagccaa gatgacctaa gttttctggc aaacgcgcgc aatatacctc aatcaaaact
      481 agaagaagaa atgaaatcac aagctatata tagaaaagaa tacgcaataa gtaatgagaa
      541 gataaaaaac tatgctttat attatcatga ccaagccaaa aacattatac aaaaacatca
      601 agatggttcg ggaaatcaaa acgtcgatat agcatttata actgataaaa atcataaaaa
      661 acacctttct gacatattta aagtaataaa tgaatcagga gtaaaatatg atgtaattca
      721 ctttggcgct tgccgagtaa atagagagga gcaataatca tcatttacca taatccctta
      781 attgtacgca ccactgaaac acgctgaacg cgattctgac gcagacaccg caaaaagaga
      841 tgttttaccc cggaaaaaca ggaaggatgc gtcaggatcg ttttcagctc gctgcatagc
      901 tatgcatgaa agcgaatggt gatcactttg ggagcttacg gtgttcatac cgtctgtttt
      961 cgacagtttt ctctccggga agctaatctg ccataagcct ggataacagg gcacggtgat
     1021 actccgtaat agcgatcagc acctccgcat attccctgtc cccgacacgc ttgccgtcga
     1081 taaagagttt tttctgtaac gcccccagtc tcataacttc tttcagtacc cgatcatcag
     1141 tcagtgagtt aactttctta ccgagagctg ccgcccgtaa aaaaccggaa acggtcttgc
     1201 cgcattcagc agcccttttt ctgatttcct ggtcttcttc tgccgtcaga cgaacagggc
     1261 gactgatagc ccgcctgcgc gtattacttc cacttcgttt tgtcataaaa catggctccg
     1321 tatctgacat gggtgtcggg gcaaagccct gaccagggta attgtaatag cgtgcatgca
     1381 tgcgcggtat aacaattaca catcctgtcc tgtcagcaag ttcgaattac cgacaaagac
     1441 acggcagttt tttggcagtg acagtattgt caggcagaaa tacccgtcaa agcgtcagac
     1501 ggcagaatac cggcggtaaa acagtcgaaa agtggtacgc gatataatca cacaacgcag
     1561 atgacagaca gaacggagaa aacgaaaaga tgaacaagca gcagcaagcc gtactcaaca
     1621 tggcaggatt cataaaaagc cagagcctga cgctgctcga aaaactggac gcactcgatg
     1681 ctgacgaaca ggccgccatg tgtgagaagc tgcacgaact cgcggaagaa cttcagaaca
     1741 gcatacagac acgctttgaa gcggaaaatc gcaccgggat ataacgcatc cggacaggat
     1801 gcagaacggc atagaggagc gtaaggagaa aatgtcacat aaggcgctct tccgcttcct
     1861 cgctcactga ctcgctacgc tcggtcgttc gactgcggcg agcggtgtca gctcactcaa
     1921 aagcggtaat acggttatcc acagaatcag gggataaagc cggaaagaac atgtgagcaa
     1981 aaagcaaagc accggaagaa gccagcgccg caggcgtttt tccataggct ccgcccccct
     2041 gacgagcatc acaaaaatcg acgctcaagc cagaggtggc gaaacccgac aggactataa
     2101 agataccagg cgtttccccc cggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg
     2161 cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca
     2221 cgctgttggt atctcggccc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa
     2281 ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga atccaacccg
     2341 gtaagacacg acttaacgcc actggcagca gccattggta actggacaag gtggattgag
     2401 atattaagag ttcttgaagt ggtggcctaa ctgcggctac actagaagga cagtatttgg
     2461 tatctgtgct ccaccaagcc agttacccgg ttaagcagtc cccaactgac ttaaccttcg
     2521 actaaaccgc ctccccaggc ggttttttcg tttacaggca gcagattacg cgcagaaaaa
     2581 aaggatctca agaagaacct ttaatttttt ttcacacctg gtatcctact ttaacccata
     2641 aaataaaaac ttacaccgac acgttcttaa acaaaagaaa aaaaataat
//
LOCUS       DADSYM010000245         2614 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2415
            SAMN08039579-rid11723173.denovo.247, whole genome shotgun sequence.
ACCESSION   DADSYM010000245 DADSYM010000000
VERSION     DADSYM010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039579
            Sequence Read Archive: SRR6362533
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2614)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2614)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980162.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 03:39:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,484
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 4,347
            CDSs (with protein)               :: 4,347
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 43 of 85
            Pseudo Genes (incomplete)         :: 37 of 85
            Pseudo Genes (internal stop)      :: 19 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2614
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039579-rid11723173.denovo.247"
                     /strain="LGE2415"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8302 N 95.9628 W"
                     /collection_date="2015-08-24"
     gene            139..1071
                     /locus_tag="J1Y83_004243"
     CDS             139..1071
                     /locus_tag="J1Y83_004243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HBA7632322.1"
                     /translation="MANLLNKFIMTRILAAITLLLSIVLTILVTIFCSVPIIIAGIVK
                     LLLPVPVIWRKVSRFCDFMMYCWCEGLAVLLHLNPHLQWEVHGLEGLSKKNWYLLICN
                     HRSWADIVVLCVLFRKHIPMNKYFLKQQLAWVPFLGLACWALDMPFMKRYSRAYLLRH
                     PERRGKDVETTRRSCEKFRLHPTTIVNFVEGSRFTQEKHQQTHSTFQNLLPPKAAGIA
                     MALNVLGKQFDKLLNVTLCYPDNNRQPFFDMLSGKLTRIVVHVDLQPIADELHGDYIN
                     DKSFKRHFQQWLNSLWQEKDRLLTSLMSSPRQEK"
     gene            complement(1112..2542)
                     /locus_tag="J1Y83_004244"
     CDS             complement(1112..2542)
                     /locus_tag="J1Y83_004244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdgA family protein"
                     /protein_id="HBA7632323.1"
                     /translation="MIRKSATGAIVALAVIWGGGTWYTGTQIQPGVEKFIKGFNDAKK
                     KGEHAYDMTLSYKNFDKGFFNSRFQMQMTFDNGAPDLNIKPGQKIVFDVDVEHGPLPI
                     TMLMHGNVIPALAAAKVNLVNNELTQPLFIAAKNKSPVEATLRFAFGGSFSTTLDVAP
                     AEYGKFSFGEGQFTFNGDDSSLSNLDIEGKVEDIVLQFSPMNKVTAKSFTIDSLTRLE
                     EKKFPVGESESKFNQVNIINQGEVVAQIDAFVAKTRLDRVKDKDYINVNLTYELDKLT
                     KGNQQLGSGEWSLIAESIDPSAVRQFIIQYNIAMQKQLAAHPELANDEVALQEVNAAL
                     FKEYLPLLQKSEPTIKQPVRWKNALGELNANLDISIADPAKSSSSTNKDIKSLNFDMK
                     LPLNVATETAKQLNLSEGMDAEKAQKRADKQISGMMTLGQMLQLITIDNNTASLQLRY
                     TPGKVFFNGQEMSEEEFMSRAGRFVH"
BASE COUNT          687 a          574 c          541 g          812 t
ORIGIN      
        1 agccatccct aataattgag ataatgggat tttttctaat gtggatagat atgaattatt
       61 tttctcctta aggatcatcc gttatttggg tcgttttctg ctaaaatctg cgccttgcac
      121 ttctttagcg cactgtctat ggctaacctt ttgaataaat tcattatgac gagaatactc
      181 gctgcgataa cccttttgct gagtatcgta ttgaccattt tggtcactat tttttgttcc
      241 gtcccgatca tcattgccgg gattgtaaaa ctattgctac ctgtgccagt catttggcga
      301 aaggtttcgc gtttttgtga ttttatgatg tattgctggt gtgaaggtct ggcggtatta
      361 ctgcatctga acccacacct gcaatgggaa gttcacgggc tggaagggtt aagtaagaag
      421 aactggtatc tacttatttg taaccaccgt agttgggcag atattgtcgt actgtgcgtg
      481 ctgtttcgta agcacattcc gatgaataaa tatttcctca agcagcagct ggcctgggtg
      541 ccttttcttg gcctggcgtg ctgggcgctg gatatgccgt ttatgaagcg ttattcccgc
      601 gcttatttat tacgtcatcc ggagcgacgt ggtaaagatg ttgaaaccac tcggcgttct
      661 tgtgaaaagt ttcgcctgca tcccaccact attgtcaatt tcgttgaagg ctcccgtttc
      721 acgcaagaaa aacatcagca aacccactcc acttttcaaa acttgttgcc accaaaggcg
      781 gcaggcattg cgatggcgct aaatgtactg ggtaaacaat tcgataaact gcttaatgtt
      841 acgctgtgtt atccggacaa taaccgtcag ccgttcttcg atatgttaag cggtaaactg
      901 acgcgaattg tcgtacatgt ggatttacag cctattgccg atgagttaca tggcgactac
      961 atcaatgaca aaagctttaa gcgccatttt cagcaatggc tgaactcatt gtggcaggaa
     1021 aaagaccggc ttctgacatc gttgatgtca tcaccgcggc aggaaaagta agttttactc
     1081 gcctctgaat gagcagaggc gagtgagtat tttaatgaac aaaacgtccg gcacgagaca
     1141 taaattcttc ttcgctcatc tcctgaccgt taaaaaaaac tttacccggt gtataacgca
     1201 gttgcagcga ggcggtattg ttgtcaatcg tgattaactg caacatctga cctaatgtca
     1261 tcatcccgct gatttgttta tcagcccgct tttgcgcttt ttccgcatcc atcccttcag
     1321 ataaattaag ctgttttgcg gtttctgtgg cgacattaag cggtaacttc atatcaaaat
     1381 tgagcgattt aatatctttg tttgtggatg atgaagattt ggctgggtcg gcaatactga
     1441 tatccagatt ggcatttagt tcgccgagtg cgttcttcca tcttaccggt tgtttaatgg
     1501 tcggctcact tttttgtaat aacggtaaat actctttgaa caatgcagca ttcacttctt
     1561 gcagagcaac ttcatcgttt gctaactcag ggtgtgcagc aagctgcttc tgcatcgcaa
     1621 tgttatactg gatgataaat tggcgcaccg ctgagggatc aatagactca gcaatcaatg
     1681 accactcacc actaccgagt tgctgattcc cttttgttaa cttatcaagt tcgtaggtca
     1741 gattgacatt gatataatct ttgtctttaa cgcgatccag cctggttttt gcaacgaaag
     1801 catcgatttg ggcaacgact tccccttggt tgataatatt aacctgatta aatttcgact
     1861 cgctttcacc aaccggaaat ttcttttctt ctaatcgtgt cagagaatca atggtaaaac
     1921 ttttcgccgt tactttgttc attggtgaaa attgcagaac aatatcttcg actttgcctt
     1981 caatatccag gttagacaat gaactatcat caccattaaa agtaaactgg ccttcaccaa
     2041 aagaaaactt tccatactct gcaggggcaa catctaatgt cgtggagaat gagccaccaa
     2101 acgcgaatcg caatgtcgct tccacgggcg atttattttt cgcggcgata aatagcggtt
     2161 gtgtcagctc attattcact aagttcactt ttgccgctgc cagtgctggg atgacattac
     2221 catgcattaa cattgtgatg ggcaacggac cgtgctcaac atccacatca aatacaattt
     2281 tctggcctgg cttgatattg agatcgggtg caccgttatc aaatgtcatt tgcatttgaa
     2341 aacgggaatt aaaaaagcct ttgtcaaaat ttttataact taacgtcata tcgtaggcat
     2401 gttcaccttt ctttttagca tcgttaaaac ctttaataaa cttttcgaca ccaggctgaa
     2461 tttgcgtacc tgtgtaccat gtgccaccac cccagattac ggctaacgca acaatggcac
     2521 ctgtagctga cttacgtatc atataaatat tccctgataa aacagcaaaa ctttgaatat
     2581 ccacttatgc tgaaataatg tccagcagca ggaa
//
LOCUS       DADSYM010000246         2455 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2415
            SAMN08039579-rid11723173.denovo.248, whole genome shotgun sequence.
ACCESSION   DADSYM010000246 DADSYM010000000
VERSION     DADSYM010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039579
            Sequence Read Archive: SRR6362533
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2455)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2455)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980162.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 03:39:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,484
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 4,347
            CDSs (with protein)               :: 4,347
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 43 of 85
            Pseudo Genes (incomplete)         :: 37 of 85
            Pseudo Genes (internal stop)      :: 19 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2455
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039579-rid11723173.denovo.248"
                     /strain="LGE2415"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8302 N 95.9628 W"
                     /collection_date="2015-08-24"
     gene            326..469
                     /locus_tag="J1Y83_004245"
     CDS             326..469
                     /locus_tag="J1Y83_004245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YobF family protein"
                     /protein_id="HBA7632324.1"
                     /translation="MCGIFSKEVLSKHVDVEYRFSAEPYIGASCSNVSVLSMLCLRAK
                     KTI"
     gene            482..691
                     /gene="cspE"
                     /locus_tag="J1Y83_004246"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
     CDS             482..691
                     /gene="cspE"
                     /locus_tag="J1Y83_004246"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator/RNA stability
                     regulator CspE"
                     /protein_id="HBA7632325.1"
                     /translation="MAKIKGQVKWFNESKGFGFITPADGSKDVFVHFSAIQGNGFKTL
                     AEGQNVEFEIQDGQKGPAAVNVTAI"
     gene            857..1666
                     /gene="rlmA"
                     /locus_tag="J1Y83_004247"
     CDS             857..1666
                     /gene="rlmA"
                     /locus_tag="J1Y83_004247"
                     /EC_number="2.1.1.187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (guanine(745)-N(1))-methyltransferase"
                     /protein_id="HBA7632326.1"
                     /translation="MSFSCPLCHQPLSREKNSYICPQRHQFDMAKEGYVNLLPVQHKR
                     SRDPGDSAEMMQARRAFLDAGHYQPLRDAIVGQLRERLDAKAAAVLDIGCGEGYYTHA
                     FADALPEITTFGLDVSKVAIKAAAKRYPQVTFCVASSHRLPFSDTSMDAIIRIYAPCK
                     AEELVRVVKPGGWVITATPGPRHLMELKGLIYNEVHLHAPHAEQLKGFTLQQSDELCY
                     LMRLRGDEAVALLQMTPFAWRAKPEVWQALAAKEVFDCQTDFNIHLWQRSY"
     gene            complement(1663..2229)
                     /gene="mntP"
                     /locus_tag="J1Y83_004248"
     CDS             complement(1663..2229)
                     /gene="mntP"
                     /locus_tag="J1Y83_004248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese efflux pump MntP"
                     /protein_id="HBA7632327.1"
                     /translation="MNITATVLLAFGMSMDAFAASIGKGATLHKPKFSEALRTGLIFG
                     AVETLTPLIGWGMGMLASRFVLEWNHWIAFVLLIFLGGRMIIEGFRGADDEDEEPRRR
                     HGFWLLVTTAIATSLDAMAVGVGLAFLQVNIIATALAIGCATLIMSTLGMMVGRFIGS
                     IIGKKAEILGGLVLIGIGVQILWTHFHG"
BASE COUNT          655 a          577 c          595 g          628 t
ORIGIN      
        1 atgagaaaaa gtgatataac tttttattca ttgcatagta gaaaatgtga tattgcacgc
       61 actatgtaat aacttctccc actggcctgg aacaactgaa cttattgaac tatgttagaa
      121 aatacgtcag tttaagtatc tgcctgaact ggcaaggtta agcacaatga tatatcggcg
      181 cgtattccgt tgcataagtg tgcaaaaaaa gtgaaagacg tatcgagatt tgtgcgtctg
      241 atcgagacat gtttaaaaat ggcttgccat aattaacgtt gtatgtgata acagatttcg
      301 ggttaaacga ggtacagttc tgtttatgtg tggcattttc agtaaagaag tcctgagtaa
      361 acacgttgac gttgaatacc gcttctctgc cgagccttat attggtgcct catgcagtaa
      421 tgtgtcagtt ttatctatgt tatgcctgcg ggcgaagaaa acaatctaag gaatttttca
      481 aatggcaaag attaaaggtc aggttaagtg gttcaacgag tctaaaggtt ttggcttcat
      541 tactccggct gatggcagca aagatgtgtt cgtacacttc tccgctatcc agggtaatgg
      601 cttcaaaact ctggctgaag gtcagaacgt tgagttcgaa attcaggacg gtcagaaagg
      661 tccggcagct gttaacgtaa cagctatctg atcgaatcca ctgatctgaa gtgtgaatac
      721 gcttcaatct cgctataaag cctcgtcgaa tgcgaggctt tttactttgt tttatcttcg
      781 ctccagtcgt tcggatattt gcccgccgca tgattcgcgt tacacttgcg gcctttagta
      841 tcctgccgga gttgtcatgt cgttttcctg tccactttgc catcagcctc tttcgcgtga
      901 aaaaaacagc tatatctgtc cccagcgaca tcagtttgat atggcgaaag aagggtatgt
      961 caatctgctg cccgttcagc ataaacggtc tcgtgatccg ggcgacagcg cggaaatgat
     1021 gcaagcacgc cgcgcattct tagatgccgg acattatcag ccgctgcgtg atgcaattgt
     1081 cggccaactg cgggaacggc ttgatgcaaa ggccgcggcg gtgctggata ttggctgtgg
     1141 tgaagggtat tacacacacg catttgctga tgcgttgccc gaaatcacca cgtttggtct
     1201 ggatgtctcg aaggtagcga taaaagcggc ggcgaaacgc tatccgcagg tcactttttg
     1261 tgttgcttcc agccaccgtt tgccgttttc cgataccagt atggacgcca taatacgtat
     1321 ttacgcgccg tgtaaagcag aagaattagt gcgagtagtg aagcccggcg gttgggtcat
     1381 tactgccacg ccgggaccga gacacttaat ggagctgaag gggctgattt acaatgaagt
     1441 acatcttcat gcacctcatg cagaacaact gaaaggtttt acattacagc agagtgatga
     1501 gttgtgttat ctgatgcgtc ttcgcggtga tgaagccgtc gcattattgc agatgacgcc
     1561 gtttgcctgg cgtgcgaagc ctgaagtctg gcaagcactg gccgcaaaag aagtgttcga
     1621 ctgccagacg gactttaata ttcacctctg gcagcgttct tattaaccgt ggaagtgcgt
     1681 ccagaggatc tggacgccaa taccgatcag taccagcccg ccgagaattt ccgctttttt
     1741 cccaataatt gagccgataa agcgaccaac catcatccct aatgtcgaca taatcaaggt
     1801 tgcacaacca atggccaatg cggtcgcgat aatgttaacc tgcaggaaag caagaccaac
     1861 acccacagcc atggcatcca ggctggtggc aatcgcagtg gttaccagta accagaaacc
     1921 gtgtcgacgg cgcggttctt catcttcatc atctgcgcca cgaaaaccct caataatcat
     1981 tcgcccgccg agaaatatca gcagcacaaa cgcaatccag tggttccatt caaggacaaa
     2041 ccggctggct aacatgccca ttccccagcc gatcagcggc gtcagggttt cgacggcacc
     2101 aaaaataagg ccggttcgca atgcttcaga aaatttcggt ttatggaggg tggcaccttt
     2161 accgattgat gcagcaaagg catccatcga cataccaaac gcaagaagaa cagtagcagt
     2221 gatattcatg acaacgtcct gaccggggta tccataaaac acatcatcgc ccccagcaaa
     2281 catgcataca tgaacaacaa gccgcgatgg ttttcgcgtt catgcaggca tagcgattga
     2341 tgtgcatatg gtctcgcctg actgaaagtg ttcagtccgt acgtgccacg ttttgcaacg
     2401 agtatgttga cacgtacatt tccggaatct ggaaatcagc tactccccaa atgag
//
LOCUS       DADSYM010000247         2422 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2415
            SAMN08039579-rid11723173.denovo.249, whole genome shotgun sequence.
ACCESSION   DADSYM010000247 DADSYM010000000
VERSION     DADSYM010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039579
            Sequence Read Archive: SRR6362533
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2422)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2422)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980162.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 03:39:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,484
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 4,347
            CDSs (with protein)               :: 4,347
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 43 of 85
            Pseudo Genes (incomplete)         :: 37 of 85
            Pseudo Genes (internal stop)      :: 19 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2422
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039579-rid11723173.denovo.249"
                     /strain="LGE2415"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8302 N 95.9628 W"
                     /collection_date="2015-08-24"
     gene            complement(163..1536)
                     /gene="cobA"
                     /locus_tag="J1Y83_004249"
     CDS             complement(163..1536)
                     /gene="cobA"
                     /locus_tag="J1Y83_004249"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HBA7632328.1"
                     /translation="MDHLPIFCQLRDRDCLIVGGGDVAERKARLLLDAGARLTVNALA
                     FIPQFTAWADAGMLTLVEGPFDESLLDTCWLAIAATDDDALNQRVSEAAEARRIFCNV
                     VDAPKAASFIMPSIIDRSPLMVAVSSGGTSPVLARLLREKLESLLPLHLGQVAKYAGQ
                     LRGRVKQQFATMGERRRFWEKLFVNDRLAQSLANNDQKAITETTEQLINEPLDHRGEV
                     VLVGAGPGDAGLLTLKGLQQIQQADVVVYDRLVSDDIMNLVRRDADRVFVGKRAGYHC
                     VPQEEINQILLREAQKGKRVVRLKGGDPFIFGRGGEELETLCNAGIPFSVVPGITAAS
                     GCSAYSGIPLTHRDYAQSVRLITGHLKTGGELDWENLAAEKQTLVFYMGLNQAATIQQ
                     KLIEYGMPGEMPVAIVENGTAVTQRVIDGTLTQLGELAQQMNSPSLIIIGRVVGLRDK
                     LNWFSNH"
     gene            complement(1555..2361)
                     /gene="nirC"
                     /locus_tag="J1Y83_004250"
     CDS             complement(1555..2361)
                     /gene="nirC"
                     /locus_tag="J1Y83_004250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite transporter NirC"
                     /protein_id="HBA7632329.1"
                     /translation="MFTDTINKCAANAARIARLSANNPLGFWVSSAMAGAYVGLGIIL
                     IFTLGNLLYPSVRPLVMGATFGIALTLVIIAGSELFTGHTMFLTFGVKAGTISHGQMW
                     AILPQTWLGNLVGSVFVAMLYSWGGGSLLPVDTSIVHSVALAKTTAPAMVLFFKGALC
                     NWLVCLAIWMALRTEGAAKFIAIWWCLLAFIASGYEHSIANMTLFALSWFGNHSEAYT
                     LAGIGHNLLWVTLGNTLSGAVFMGLGYWYATPKANRPVADKFNQTETAAG"
BASE COUNT          622 a          664 c          597 g          539 t
ORIGIN      
        1 caattagcgt cagtgagcga ttgatttaat tttgtaagtc gctgtaacgg tgaatataac
       61 ccattagcag acaatattta ttaaggggtt atgtttttat ctgctgaata tataaagaga
      121 gttgtaaacg aaaaaggcca gacttctctg gccttggtta aattaatggt tagagaacca
      181 gttcaattta tcgcgcaggc caacaacccg accaataata attagcgatg gactgttcat
      241 ttgctgcgcc agttcgccca gctgcgtgag cgtaccgtca atcacgcgct gcgtgactgc
      301 cgttccgttt tcgacaattg ccaccggcat ttcaccaggc ataccgtatt caatcagctt
      361 ttgctgaata gttgcagcct gattcagccc catataaaac accagcgtct gtttttctgc
      421 cgccaggttt tcccagtcca gctcgccacc ggtttttaag tgtccagtaa ttaagcgtac
      481 gctctgggca taatcgcgat gcgtgagcgg aatacccgaa taggcagagc aaccagaagc
      541 tgcggtaata cccggaacca ccgagaatgg aatacccgcg ttgcacagtg tttccagctc
      601 ttctccgcca cgcccgaaaa taaacggatc gccgcctttc agacgcacca cgcgtttacc
      661 tttttgcgct tcccgcagca agatctggtt aatctcttcc tgcggtacgc agtggtatcc
      721 cgcgcgtttg ccgacgaaaa cgcgatcagc atcgcggcgt accagattca taatatcgtc
      781 agaaaccaga cggtcgtaga ccaccacatc tgcctgctga atttgttgca gacctttcag
      841 tgtcagcagc ccggcatcgc ccggacctgc accaaccagc accacttcac cgcgatggtc
      901 gagcggttcg ttgattaact gttcggtggt ttcagtaatg gctttctgat cgttgttggc
      961 cagcgactgc gccaggcggt cgttaacgaa taatttctcc cagaaacggc gacgctcacc
     1021 catcgtggcg aactgttgtt tcactcgccc gcgtaattgc ccggcgtatt tcgctacctg
     1081 gcccagatgt aacggcagca gtgattcgag tttttcgcgc aacaggcgcg ccagaaccgg
     1141 agaggtgccg ccagaggaga ccgctaccat gagcggcgag cggtcaataa tcgacggcat
     1201 aataaagctg gcggctttcg gcgcatcgac cacgttacag aagatgcgac gagcttcagc
     1261 ggcttcgctg acgcgctggt taagcgcgtc atcatctgtc gctgcaatcg ccagccagca
     1321 ggtgtcgaga aggctttcat caaatggccc ttcgacgagg gttaacatgc ctgcatctgc
     1381 ccatgcggtg aactgtggaa taaacgctaa tgcattcacc gttaagcgag cgcctgcgtc
     1441 taacagcaac cttgctttgc gttccgcgac atcaccaccg ccgacaatca gacagtcgcg
     1501 atcgcgtaat tggcaaaata taggcaaatg atccacgtaa aaacccctta gtaattaacc
     1561 ggcagccgtt tcagtttgat taaatttgtc cgcaaccgga cgattcgctt tcggcgtagc
     1621 ataccaataa cccaatccca tgaatacggc acccgataaa gtattaccca gcgtcaccca
     1681 cagcaggtta tgaccaatgc ccgccagcgt gtaggcttcg ctgtggttgc cgaaccagga
     1741 gagtgcgaac agcgtcatgt tagcgatgga gtgctcgtag ccggacgcga taaatgccag
     1801 cagacaccac cagattgcga taaatttcgc cgcgccttct gtacgcaatg ccatccagat
     1861 agccagacaa accagccagt tacacaatgc gcctttgaag aataatacca ttgccggtgc
     1921 ggtggtttta gccagcgcga cggagtgaac gatgctggta tcgaccggca gcaggctacc
     1981 gccgccccag ctataaagca tggcaacgaa gacggaaccg accaggttac ccagccaggt
     2041 ttgcggcagg attgcccaca tttgcccgtg gctgatggtg cccgctttca ctccaaaggt
     2101 gaggaacatg gtgtgtccgg tgaacagttc agaaccggcg ataatcacca gcgttaaggc
     2161 gataccaaag gtcgcaccca tcaccagagg acgtacggac gggtagagca aattaccgag
     2221 cgtgaaaatc aggatgatcc caagacccac atacgccccc gccatggcgg agctgaccca
     2281 aaagccgagc gggttatttg ccgacaggcg tgcaatgcgc gcagcgttag ccgcacactt
     2341 attaatagtg tctgtaaaca tttgattatc ctttaaaaaa taaaaacaaa aaataaaaca
     2401 aaaagtttaa aaaatgtcct gc
//
LOCUS       DADSYM010000248         2337 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2415
            SAMN08039579-rid11723173.denovo.250, whole genome shotgun sequence.
ACCESSION   DADSYM010000248 DADSYM010000000
VERSION     DADSYM010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039579
            Sequence Read Archive: SRR6362533
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2337)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2337)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980162.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 03:39:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,484
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 4,347
            CDSs (with protein)               :: 4,347
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 43 of 85
            Pseudo Genes (incomplete)         :: 37 of 85
            Pseudo Genes (internal stop)      :: 19 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2337
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039579-rid11723173.denovo.250"
                     /strain="LGE2415"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8302 N 95.9628 W"
                     /collection_date="2015-08-24"
     gene            <1..423
                     /locus_tag="J1Y83_004251"
     CDS             <1..423
                     /locus_tag="J1Y83_004251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001042470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose mannosyl hydrolase"
                     /protein_id="HBA7632330.1"
                     /translation="VEHTPLISIDLIIRNDKGEALLGQRLNRPAQNYWFVPGGRIYKD
                     ESFEIAFKRITLEELGVQISLNDALFLGVYEHFYNDNFFEAEFSTHYIVHGYEIQLKP
                     QQLHLPTVQHNSYKWFDVVTLLNSTIVHQYTKNYFIPR"
     gene            429..1835
                     /locus_tag="J1Y83_004252"
     CDS             429..1835
                     /locus_tag="J1Y83_004252"
                     /EC_number="2.7.7.13"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001610403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate
                     guanylyltransferase/mannose-6-phosphate isomerase"
                     /protein_id="HBA7632331.1"
                     /translation="MLLPVVMAGGSGTRLWPLSRTLYPKQFLSLNSRLTMLQETLRRL
                     DKVEHKPALVICNESHRFIVAEQLRKESLKHSGILLEPVGRNTAPAVALAALQAMVTG
                     DDPILLVLAADHEIQDEDNFIDAVLVAKNFAEQGKLVTFGIVPTSPETGYGYIKSGES
                     LDGQGYKVAAFVEKPDLHVAQRYISDGGYLWNSGMFMFRASVFIDELKKFRPDILASC
                     QRSLSSSIQDLDFIRLDNASFSCCPEESIDYAVMEKTAEAVVVPLNAQWSDVGSWSAL
                     WEISSKDQSGNAIRGDVLVEDATDSYLYSQHRLIGAVGVKDLVVVETKDAVLVAHKDK
                     VQQVKNIVAQLKKNNRTEYLQHREIFRPWGSHDTIAEGPRFQVKHVIVLPGHITAKQI
                     HYHRTEHWIVVSGTAKVHLEDKTYLVSENESTYIPVGVPHAIENPGKIPLEIIEVRSG
                     VYLEEDDVIRVSSSGVGY"
     gene            1835..>2337
                     /locus_tag="J1Y83_004253"
     CDS             1835..>2337
                     /locus_tag="J1Y83_004253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001222848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HBA7632332.1"
                     /translation="MRISIITATYNSEKTLLDTLLSLEKQTHPDIEYIVVDGASKDNT
                     IELIKSNCTRVSKIICEPDHGIYDALNKGMQAASGDVIGFLHSDDLLAYDDAIADIAK
                     TFESTGCDAIYGDLEYVAQNDTTKRIRLWKSGSFSRFKMKVGWMPPHPSFYMKRECYS
                     QFGSFSL"
BASE COUNT          684 a          410 c          511 g          732 t
ORIGIN      
        1 gttgagcata ctcctctaat ctcgattgat ctcataatta gaaacgataa aggagaggcg
       61 ctgctggggc agcgcctgaa tcgcccagca caaaattatt ggtttgtgcc aggagggcga
      121 atttataagg atgagtcatt cgagattgca tttaagcgga taacacttga agagttgggc
      181 gttcaaatta gtcttaatga tgccttattt cttggggtgt atgaacattt ttacaatgat
      241 aatttttttg aagcagaatt ttctacacac tatatagtgc atggatatga aatccaactt
      301 aaacctcagc aacttcacct accaacggtc cagcataatt cctacaagtg gtttgatgta
      361 gtaacgttgc ttaatagcac tatagttcat caatatacca aaaattattt tataccaagg
      421 taatagatat gctacttccc gttgtcatgg ccggtggttc tggcaccaga ttatggcctc
      481 tttcacgtac actttatccg aaacaatttc tgtctttaaa tagtcgttta accatgttgc
      541 aagagacatt gaggcggctt gacaaggtcg aacataaacc tgctttggtc atttgtaacg
      601 aatcacatcg ctttatcgtt gctgaacaat tgcgtaaaga gagtttaaag catagcggta
      661 ttttgcttga gcctgttggc cgtaatactg cgcctgctgt agcacttgca gcacttcagg
      721 ctatggtgac tggagacgat cctattctgt tggttcttgc tgctgatcat gaaatccagg
      781 atgaggataa tttcattgat gcagttcttg tagccaagaa ttttgcagag cagggtaagc
      841 ttgttacgtt tggtattgtt ccaacatccc cagagactgg ctatggttac attaagtcag
      901 gtgaatctct ggatggacaa ggttacaaag ttgcggcttt tgttgaaaaa ccagatcttc
      961 acgtggctca gcggtacata tcagatggcg gttatctttg gaatagtggg atgtttatgt
     1021 tcagagcatc tgtatttatc gatgaactga aaaaattccg accagatatt ttagccagtt
     1081 gccaacgctc cttgtcctct tcgatacaag atttagattt tatccgtctg gataatgctt
     1141 cattttcttg ctgtcctgaa gagtctattg actatgcagt tatggaaaaa acagccgaag
     1201 ctgtcgtcgt tccattaaat gcacaatgga gtgatgttgg gtcatggtct gcattgtggg
     1261 aaataagttc aaaagatcaa agcggtaatg ccattcgtgg tgatgtattg gttgaagatg
     1321 ctacagatag ttatctttat tcgcagcata gacttattgg tgccgtgggc gtaaaggatt
     1381 tggttgttgt tgaaacgaaa gatgccgtat tagttgctca taaagataaa gttcagcaag
     1441 ttaaaaacat cgtcgctcaa cttaaaaaaa ataatcgcac agaatattta cagcatcggg
     1501 aaatatttcg gccttggggc agtcatgata ctatagctga agggccgcgc tttcaagtga
     1561 aacatgtgat tgtattacct ggccacatta ccgctaaaca gattcattac catcgtactg
     1621 agcattggat tgttgtctcg gggacagcta aagttcatct tgaagataag acttaccttg
     1681 tttccgaaaa tgaatcaaca tatatacctg ttggtgttcc acatgccatt gaaaatcctg
     1741 gcaagatacc gcttgaaata attgaggtta gatcaggagt ctacctagaa gaggatgatg
     1801 ttataagagt ttcttcttct ggagtaggat actaatgcga atttctatta tcacagccac
     1861 ttataatagt gaaaaaactc tccttgatac attactttct ctagaaaaac aaacgcatcc
     1921 agatatcgaa tatatagttg tagatggagc atcgaaagat aatacaattg agctgatcaa
     1981 gagtaattgt acaagagttt caaaaatcat ttgtgaaccc gatcatggca tttatgatgc
     2041 tctgaataaa ggtatgcaag cagcttcggg tgatgttatt ggttttttac attctgatga
     2101 tttacttgct tatgatgatg ctattgcaga tatagcaaaa acatttgaaa gtacagggtg
     2161 tgatgctatt tatggcgatt tggagtatgt tgcccaaaat gatacgacta aacgtattag
     2221 attatggaaa agtggctcat ttagtcgctt caagatgaaa gtggggtgga tgccaccaca
     2281 cccatcattt tatatgaaac gggaatgtta tagtcaattt ggttcttttt ctttaga
//
LOCUS       DADSYM010000249         2247 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2415
            SAMN08039579-rid11723173.denovo.251, whole genome shotgun sequence.
ACCESSION   DADSYM010000249 DADSYM010000000
VERSION     DADSYM010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039579
            Sequence Read Archive: SRR6362533
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2247)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2247)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980162.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 03:39:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,484
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 4,347
            CDSs (with protein)               :: 4,347
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 43 of 85
            Pseudo Genes (incomplete)         :: 37 of 85
            Pseudo Genes (internal stop)      :: 19 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2247
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039579-rid11723173.denovo.251"
                     /strain="LGE2415"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8302 N 95.9628 W"
                     /collection_date="2015-08-24"
     gene            complement(<1..460)
                     /locus_tag="J1Y83_004254"
     CDS             complement(<1..460)
                     /locus_tag="J1Y83_004254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310129.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HBA7632333.1"
                     /translation="MHVTAKPSSFQCNLKCDYCFYLEKESQFTHEKWMDDSTLKEFIK
                     QYIAASGNQVYFTWQGGEPTLAGLDFFRKVIHYQQRYAGQKRIFNALQTNGILLNNEW
                     CAFLKEHEFLVGISIDGPQELHDRYRRSNSGNGTFAKVIAAIERLKSYQIE"
     gene            complement(512..2194)
                     /locus_tag="J1Y83_004255"
     CDS             complement(512..2194)
                     /locus_tag="J1Y83_004255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310130.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HBA7632334.1"
                     /translation="MKSALKKSVVSTSISLILASGMAAFAAHAADDVKLKATKTNVAF
                     SDFTPTEYSTKGKPNIIVLTMDDLGYGQLPFDKGSFDPKTMENREVVDTYKIGIDKAI
                     EAAQKSTPTLLSLMDEGVRFTNGYVAHGVSGPSRAAIMTGRAPARFGVYSNTDAQDGI
                     PLTETFLPELFQNHGYYTAAVGKWHLSKISNVPVPEDKQTRDYHDNFTTYSAEEWQPQ
                     NRGFDYFMGFHAAGTAYYNSPSLFKNRERVPAKGYISDQLTDEAIGVVDRAKTLDQPF
                     MLYLAYNAPHLPNDNPAPEQYQKQFNTGSQTADNYYASVYSVDQGVKRILEQLKKNGQ
                     YDNTIILFTSDNGAVIDGPLPLNGAQKGYKSQTYPGGTHTPMFMWWKGKLQPGNYDKL
                     ISAMDFYPTALDAADISIPKDLKLDGVSLLPWLQNKKQGEPHKNLTWITSYSHWFDEE
                     NIPFWDNYHKFVRHQSDDYPHNPNTEDLSQFSYTVRNNDYSLVYTVENNQLGLYKLTD
                     LQQKDNLAAANPQVVKEMQGVVREFIDNSQPPLSEVNQEKFNNIKKALSKAK"
BASE COUNT          582 a          471 c          505 g          689 t
ORIGIN      
        1 actctatttg atatgatttc agacgctcga tggctgctat cacttttgca aaagtaccgt
       61 tacctgaatt actgcgtctg tagcggtcat gtagttcttg gggtccatcg atcgagatac
      121 caaccagaaa ctcatgttct ttgagaaagg cacaccattc attattcaat aaaatgccat
      181 tcgtttgcaa tgcattaaaa attcgttttt ggcctgcata gcgttgttga tagtgaataa
      241 ctttacggaa aaaatccagg ccagccagag tgggttcacc gccttgccag gtaaaataga
      301 cctgattgcc agacgctgcg atatattgtt tgatgaactc tttcagagtg ctgtcatcca
      361 tccatttttc atgagtaaac tgcgactctt tttcaaggta aaaacagtaa tcacatttga
      421 gattacattg aaaactggag ggcttggctg taacgtgcat cgctatctcg ctcaataagg
      481 cggcggaaaa atccgccgca tgaaggttta gttatttcgc tttgcttaat gctttcttga
      541 tattgttaaa cttctcctga tttacctcgc taagcggtgg ctggctgtta tcgataaact
      601 ctcttaccac gccttgcatc tctttaacga cctgcggatt ggcggcggca aggttatctt
      661 tttgctgtag atccgtcagt ttgtatagac ctaactgatt attttctact gtatagacga
      721 gcgaataatc gttatttctc accgtataag agaattggct taagtcctcg gtgttggggt
      781 tatgcgggta atcgtctgac tgatggcgga caaatttgtg gtaattatcc cagaatggaa
      841 tgttttcctc atcaaaccag tgagaataag atgttatcca ggtcagattt ttatgtggct
      901 cgccctgttt cttattttgc aaccagggca gcaaggaaac gccatccagc ttaaggtctt
      961 ttggaatgct gatatcggct gcatcaagag ctgtcgggta gaaatccatt gcggaaatca
     1021 gcttgtcata attaccgggt tgaagttttc ctttccacca cataaacatt ggggtgtgag
     1081 taccgccagg ataggtctga ctcttatagc ctttttgcgc cccgttcagc ggcagaggac
     1141 catcgataac tgcaccatta tcggaggtaa agagaataat tgtattgtca tactgtccgt
     1201 ttttcttcag ttgttcgaga atgcgtttta caccctgatc aacagaataa acggaagcgt
     1261 agtaattatc tgctgtttga ctgccggtat taaattgctt ctgatattgc tccggagcag
     1321 gattatcatt tggcagatgc ggagcattat aagccaggta aagcataaaa ggctggtcaa
     1381 gtgttttggc acgatcaaca acgccaattg cctcatcggt taactgatcg ctgatataac
     1441 cttttgcggg gacgcgttca cggtttttga acagtgaagg ggagttgtaa tatgccgttc
     1501 ctgcagcgtg gaatcccata aagtaatcaa agccacggtt ttgcggttgc cattcttccg
     1561 cagaataggt ggtgaagttg tcatgatagt cacgcgtttg tttatcttcc ggtaccggca
     1621 cattactgat ttttgacaag tgccatttac ctactgctgc agtgtaataa ccatgattct
     1681 ggaataattc aggcaggaaa gtttctgtta gcggaatacc atcctgagca tcggtattgg
     1741 aatagacacc aaagcgggcg ggcgctcgac cggtcattat tgcggcgcgg gaggggccgg
     1801 aaacaccgtg cgccacatag ccgttagtaa aacgtacgcc ttcatccatt aatgacagga
     1861 gcgtcggcgt tgatttttgc gcagcttcaa tggctttatc tatccctatt ttgtaggtat
     1921 cgacaacttc acgattttcc attgtttttg ggtcaaaaga tcccttatca aaaggaagtt
     1981 gtccataacc aagatcatcc atagtcagta cgataatatt tggctttccc ttggtactgt
     2041 attcagtcgg cgtaaagtct gagaaagcaa cgtttgtttt ggttgctttc agctttacat
     2101 catctgccgc atgagcagca aatgcagcca taccagatgc cagtatcaaa gatatcgagg
     2161 tacttacgac acttttcttt aatgcagact tcataaatgt tcctcttctt attgtactta
     2221 attccaaagt aattttacga agagaaa
//
LOCUS       DADSYM010000250         2228 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2415
            SAMN08039579-rid11723173.denovo.252, whole genome shotgun sequence.
ACCESSION   DADSYM010000250 DADSYM010000000
VERSION     DADSYM010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039579
            Sequence Read Archive: SRR6362533
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2228)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2228)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980162.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 03:39:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,484
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 4,347
            CDSs (with protein)               :: 4,347
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 43 of 85
            Pseudo Genes (incomplete)         :: 37 of 85
            Pseudo Genes (internal stop)      :: 19 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2228
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039579-rid11723173.denovo.252"
                     /strain="LGE2415"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8302 N 95.9628 W"
                     /collection_date="2015-08-24"
     gene            178..462
                     /locus_tag="J1Y83_004256"
     CDS             178..462
                     /locus_tag="J1Y83_004256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper family protein"
                     /protein_id="HBA7632335.1"
                     /translation="MADMNRTTKGALLGAGVGLLTGNGVNGVLKGAAVGAGVGAVTEK
                     GRDGKNARKGAKVGAAVGAVTGVLTGNGLEGAIKGAVIGGTGGAILGKMK"
     gene            466..801
                     /locus_tag="J1Y83_004257"
     CDS             466..801
                     /locus_tag="J1Y83_004257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmgD family protein"
                     /protein_id="HBA7632336.1"
                     /translation="MMMKKFALLAGLFVFAPMTWAQDYNIKNGLPSETYITCAEANEM
                     AKTDSAQVAEIVAVMGNASVASRDLKIEQSPELSAKVVEKLNQVCAKDPQMLLITAID
                     DTMRAIGKK"
     gene            complement(860..1973)
                     /locus_tag="J1Y83_004258"
                     /pseudo
     CDS             complement(860..1973)
                     /locus_tag="J1Y83_004258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001761115.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
BASE COUNT          595 a          510 c          483 g          640 t
ORIGIN      
        1 tcacagtcac gctcattttg aattatctga ataatagcga gcgttcttta gttaagcaga
       61 tcttaattga agacgaaatt attgtgtgtg caacttactt aataccggac aactaagttg
      121 aagaaaaaaa tacttgcctt cgggcttatc tcagcattgt tctgctccac gccagcgatg
      181 gcggatatga atcgcaccac gaaaggtgca ttgctaggcg caggcgttgg tttacttact
      241 ggcaatggtg ttaatggcgt actgaaaggt gcagctgttg gcgctggtgt tggtgcagta
      301 acagaaaaag gccgcgacgg taaaaatgcg cgtaaaggtg caaaagtggg tgccgccgtt
      361 ggtgctgtca ctggcgttct gaccggcaat ggcctcgaag gggcgattaa aggtgccgtg
      421 attggcggta cgggtggtgc aattctgggt aaaatgaaat aaggaatgat gatgaaaaaa
      481 tttgctttac tggcgggtct gtttgttttc gcaccaatga cctgggcgca ggactacaat
      541 attaagaacg gtttgccatc agaaacctat attacttgcg cagaagccaa cgaaatggca
      601 aaaactgaca gcgcacaggt tgcagaaatt gttgcggtaa tgggtaatgc cagcgttgcc
      661 agccgtgatt taaaaattga gcaatctccg gaactgagcg ccaaagtggt agaaaaactg
      721 aatcaggttt gcgctaaaga tccgcaaatg ctgttgatca cggcaattga tgacaccatg
      781 cgtgctatcg gcaaaaaata atatttcata tgttaccgcg tcgatgaatt cgacgcggtt
      841 ttttagctaa cgggcgggat cagaacttat atttcaggcc caccatcatt gcggtgtcat
      901 tgtagccatt atcacccagc tgcacgccca cattacccca gacgctggcc gccggattca
      961 gattgccatt tacgccatgt ttcatttccc ccagattacg cgccccgtct tgttcaactt
     1021 tcacaccgtt tattgagaca gcaaaatctt tactgttatg gatcccgttc atttcaacaa
     1081 aaggttcaaa gtcgtcaccg tgcggaccgc tgttttctcc tgagtacgaa taaaggtgtg
     1141 gaaaccaagt cgggtctgga tattgttatt tgcgtcattc tcaatacgtg agccattggc
     1201 ttccgtgtgc acattatgat cgacgcccat ccagatggcc tgtgccgagg tcgttctcaa
     1261 ctgtccgttg ctgtcacgcc aggcattatg cccgccaatt tgacgtagcc aaaatcggct
     1321 tgaacgctcc tgttcagtta caggatcgat atagcgcgtt tcacctgcac gatcgttcag
     1381 acgcataaca aaaagagtgt tagcggctgc caggttggcg acgtaactgc cagcttcggg
     1441 tcgcaggacg ggggtaggcg tttctgttga ttctgaagtg acattggaaa aaaataactc
     1501 tctaccattg tatatttttg caatgggggc tttagtgcca tttgttgtaa tagaactttt
     1561 ttatatcaat gttaccttgt cgaatagcaa ctgcataact tccatcagcc acagtttcaa
     1621 gtaccacata atctaaatta atatatgctt tttttccatt agcataagca ccatagctat
     1681 taataccatt agttgttatg gaaccaccat tcatttttaa atcagcacgg ctaataactt
     1741 cgtaactgtt agaatcacaa acttcatcat ctacagcatc gacacatgtt cgagactctc
     1801 caggacttga acatgcaaga actcctactg catttttccc cataggtaac aatgttcgtt
     1861 ttatctgtgg tttcaagact ggtgttattt tccacaatcc cagaatcatt aacctgactt
     1921 aaaagtcccg ctgaatatgc ccccctcgtc tcgactgaat tatcaccatt cataacgatg
     1981 gtgccaccaa attcactgac tactgcgctg gctgttatcc ctccattttc tttgtcagat
     2041 acgctttcag agcgtcttaa tattgctatc gcttatataa tactttgttt tggctatcaa
     2101 cagaaatacc ataagatcca tcaccactag tcatcacact ggcattatgt atatatccag
     2161 ttacaccgtc atccactttc acagcatggc tttcgtctta agtgattaga tactccccat
     2221 tacttgta
//
LOCUS       DADSYM010000251         2204 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2415
            SAMN08039579-rid11723173.denovo.253, whole genome shotgun sequence.
ACCESSION   DADSYM010000251 DADSYM010000000
VERSION     DADSYM010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039579
            Sequence Read Archive: SRR6362533
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2204)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2204)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980162.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 03:39:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,484
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 4,347
            CDSs (with protein)               :: 4,347
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 43 of 85
            Pseudo Genes (incomplete)         :: 37 of 85
            Pseudo Genes (internal stop)      :: 19 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2204
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039579-rid11723173.denovo.253"
                     /strain="LGE2415"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8302 N 95.9628 W"
                     /collection_date="2015-08-24"
     gene            176..2161
                     /gene="yjcS"
                     /locus_tag="J1Y83_004259"
     CDS             176..2161
                     /gene="yjcS"
                     /locus_tag="J1Y83_004259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl sulfatase YjcS"
                     /protein_id="HBA7632337.1"
                     /translation="MNNSRLFRLSRIVIAFTAASGMMINTAYATDEAKAATQYTQQVN
                     QNYAKSLPFSDRQDFDDAQRGFIAPLLDEGILRDANGKIYYRANDYKFDINAAAPETV
                     NPSLWRQSQINGISGLFKVTDKMYQVRGQDISNITFVEGEKGIIVIDPLVTPPAAKAA
                     LDLYFQHRPQKPIVAVIYTHSHADHYGGVKGIISEADVKSGKVQVIAPAGFMDEAISE
                     NVLAGNIMSRRALYSYGLLLPHNAQGNVGNGLGVTLATGDPSIIAPTKTIVRTGEKMI
                     IDGLEFDFLMTPGSEAPAEMHFYIPALKALCTAENATHTLHNFYTLRGAKTRDTSKWT
                     EYLNETLDMWGNDAEVLFMPHTWPVWGNKHINDYIGKYRDTIKYIHDQTLHLANQGYT
                     MNEIGDMIKLPPALANNWASRGYYGSVSHNARAVYNFYLGYYDGNPANLHPYGQVEMG
                     KRYVQALGGSARVINLAQEANKQGDYRWSAELLKQVIAANPGDQVAKNLQANNFEQLG
                     YQAESATWRGFYLTGAKELREGVHKFSHGTTGSPDTIRGMSVEMLFDFMAVRLDSTKA
                     AGKNISLNFNMSNGDNLNLMLNDSVLNYRKTLQSQADASFYISREDLHAVLTGQAKMA
                     DLVKAKKAKIIGNGAKLEEIIACLDNFDLWVNIVTPN"
BASE COUNT          580 a          570 c          548 g          506 t
ORIGIN      
        1 tgatagttct actgtaattt tccctcttca gcacaaatgc ctaataaaat ggctcgttcc
       61 gctcacactt ccacttatta aagtcgcaat ttcagaatta tgataacgcc agctttaaat
      121 gacgtatttt gcatggatta catatttacg acataaatga taaatggaga tttatatgaa
      181 taactctcgg ttattccgtt tgagcaggat tgttattgcg ttcactgccg ccagcggcat
      241 gatgataaat accgcttacg caacagatga agcgaaagct gccactcaat atacccaaca
      301 ggttaatcag aattacgcca aatcattacc gtttagcgat cgccaggatt ttgacgatgc
      361 tcagcgtgga tttatcgctc cgctgctgga tgaaggtatt ctgcgtgatg cgaacggtaa
      421 aatttactac cgcgcgaatg attacaaatt tgatattaat gccgctgcgc cggaaaccgt
      481 aaaccccagc ctgtggcgtc agtcgcagat caacggcatt tcaggcctgt tcaaagtcac
      541 cgataaaatg tatcaggtgc gcggccagga tatctctaac attacgttcg ttgagggcga
      601 gaaaggcatt attgttattg acccactggt gacgccacct gccgcaaaag ccgcacttga
      661 cctttacttc cagcatcgtc cgcaaaaacc gattgttgcc gttatctaca ctcacagcca
      721 cgccgaccac tatggcggcg tgaaaggtat tatctctgaa gccgatgtta aatccggcaa
      781 agttcaggtg attgctccgg ctggctttat ggacgaagcc atcagcgaaa acgtgctggc
      841 gggtaacatc atgagccgcc gtgcgctcta ctcttacggt ctgttactgc cgcacaacgc
      901 gcaaggcaat gtgggtaatg gccttggcgt gacgctggca acgggcgacc cgagcattat
      961 tgcaccgact aaaactatcg tcagaactgg cgagaagatg attatcgacg gcctggagtt
     1021 tgacttcctg atgaccccag gtagcgaagc gccagcggaa atgcacttct atattccggc
     1081 cctgaaagcc ctgtgtaccg ccgagaatgc cacgcatacc ctgcacaact tctacaccct
     1141 gcgcggcgcg aaaacccgcg acaccagcaa gtggaccgag tatctgaacg aaacattgga
     1201 tatgtggggt aacgacgcgg aagtactgtt tatgccgcac acctggccgg tctggggcaa
     1261 taagcatatc aatgattata ttggtaaata tcgcgatact atcaagtaca ttcacgacca
     1321 gaccctgcac ctggcgaacc agggctacac catgaatgaa atcggcgaca tgattaagct
     1381 gccgcctgcg cttgccaata actgggccag ccgcggctat tacggttctg tcagccacaa
     1441 cgcccgcgcg gtatataact tctatcttgg ctattacgac ggtaacccgg ctaacctgca
     1501 tccgtatggt caggtggaga tgggtaaacg ttacgtgcag gcgctgggcg gttctgcccg
     1561 cgtcatcaac ctggcgcaag aagcgaacaa gcaaggtgat tatcgctggt cggcagaact
     1621 gctgaaacag gtgattgccg ccaacccggg tgaccaggtc gcgaagaatc tgcaagcgaa
     1681 taactttgaa cagctgggct atcaggccga gtccgccaca tggcgcggtt tctacctgac
     1741 cggcgcgaaa gagctgcgcg aaggggtgca taagttcagc cacggcacca ccggttcccc
     1801 ggacaccatt cgtgggatgt cggtcgaaat gctgttcgac tttatggccg ttcgcctcga
     1861 tagcacgaaa gccgcaggta aaaatatcag cctgaacttc aatatgagca acggcgataa
     1921 cctcaacctg atgctgaacg atagcgtgct taactaccgt aaaacgctgc aatcgcaagc
     1981 cgacgcctct ttctacatta gccgtgaaga tctgcacgcc gtgctgaccg gacaggctaa
     2041 aatggccgat ctggtgaaag cgaagaaagc caaaattatt ggcaatggcg cgaaactgga
     2101 agaaattatc gcctgtctgg ataatttcga tttgtgggtg aatatcgtaa ccccaaatta
     2161 atgtcactcg cttttggggg cgctgaagcc cccaaagcat ttgc
//
LOCUS       DADSYM010000252         2155 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2415
            SAMN08039579-rid11723173.denovo.254, whole genome shotgun sequence.
ACCESSION   DADSYM010000252 DADSYM010000000
VERSION     DADSYM010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039579
            Sequence Read Archive: SRR6362533
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2155)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2155)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980162.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 03:39:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,484
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 4,347
            CDSs (with protein)               :: 4,347
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 43 of 85
            Pseudo Genes (incomplete)         :: 37 of 85
            Pseudo Genes (internal stop)      :: 19 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2155
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039579-rid11723173.denovo.254"
                     /strain="LGE2415"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8302 N 95.9628 W"
                     /collection_date="2015-08-24"
     gene            38..871
                     /gene="ppsR"
                     /locus_tag="J1Y83_004260"
     CDS             38..871
                     /gene="ppsR"
                     /locus_tag="J1Y83_004260"
                     /EC_number="2.7.11.33"
                     /EC_number="2.7.4.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="posphoenolpyruvate synthetase regulatory
                     kinase/phosphorylase PpsR"
                     /protein_id="HBA7632338.1"
                     /translation="MDNAVDRHVFYISDGTAITAEVLGHAVMSQFPVTISSITLPFVE
                     NESRARAVKDQIDAIYHQTGVRPLVFYSIVLPEIRAIILQSEGFCQDIVQALVAPLQQ
                     EMKLDPTPIAHRTHGLNPNNLNKYDARIAAIDYTLAHDDGISLRNLDQAQVILLGVSR
                     CGKTPTSLYLAMQFGIRAANYPFIADDMDNLVLPASLKPLQHKLFGLTIDPERLAAIR
                     EERRENSRYASLRQCRMEVAEVEALYRKNQIPWINSTNYSVEEIATKILDIMGLSRRM
                     Y"
     gene            1028..2074
                     /gene="aroH"
                     /locus_tag="J1Y83_004261"
     CDS             1028..2074
                     /gene="aroH"
                     /locus_tag="J1Y83_004261"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroH"
                     /protein_id="HBA7632339.1"
                     /translation="MNRTDELRTARIESLVTPAELALRYPVTPGVATHVTDSRRRIEK
                     ILNGEDKRLLVIIGPCSIHDLTAAMEYATRLQSLRNQYQPRLEIVMRTYFEKPRTVVG
                     WKGLISDPDLNGSYRVNHGLELARKLLLQVNELGVPTATEFLDMVTGQFIADLISWGA
                     IGARTTESQIHREMASALSCPVGFKNGTDGNTRIAVDAIRAARASHMFLSPDKNGQMT
                     IYQTSGNPYGHIIMRGGKKPNYHADDIAAACDTLHEFDLPEHLVVDFSHGNCQKQHRR
                     QLEVCEDICQQIRNGSTAIAGIMAESFLREGTQKIVGGQPLTYGQSITDPCLGWEDTE
                     RLVEKLASAVDTRF"
BASE COUNT          521 a          567 c          541 g          526 t
ORIGIN      
        1 tttcatagaa ttatgtctgc atcacgggaa gaacaaaatg gataatgctg ttgatcgcca
       61 cgttttttat atttctgatg gtacggcaat aactgcggag gtattaggac acgcagtaat
      121 gtcacaattt cccgtcacta tcagcagcat tacgctgccg tttgtcgaaa atgagagccg
      181 tgcacgggca gtgaaggatc agattgacgc aatttatcac cagacaggtg tgcgcccgct
      241 ggtcttctac tccatcgtgt tgccggagat tcgcgctatc atcttgcaaa gtgaaggctt
      301 ttgccaggat atcgttcagg cgctggttgc cccgctacaa caagagatga aactggatcc
      361 aacgccgatt gctcatcgta cccatggcct taaccctaat aatctcaata aatatgatgc
      421 gcgcattgcg gcgattgatt acacccttgc ccacgatgac ggtatttcgt tgcgcaatct
      481 ggaccaggct caggtgatcc tgctcggtgt ttctcgctgt ggtaaaaccc ccaccagtct
      541 gtatctggca atgcaatttg gtatccgcgc ggcaaattac ccctttattg ccgacgatat
      601 ggataatctg gtgctacccg cgtcgctcaa accacttcag cacaaattgt tcggcctgac
      661 tatcgacccg gaacgtctgg cggcgattcg cgaggaacgt cgggagaaca gtcgctatgc
      721 ctcgcttcgt cagtgcagga tggaagtcgc ggaagtggaa gcgttgtacc gtaaaaatca
      781 gatcccgtgg attaacagta ccaattattc ggtagaagag attgccacca agatcctcga
      841 tatcatgggc cttagccgcc gaatgtacta gagaactagt gcattggctt acttttttgt
      901 tatcatgcta accacccggc gaggtgtgac acacctcgca cttgaaatca gctgcgattg
      961 gtttatcgtg atgcgcatca cttcccggta gtcctgccgt agaagcaaca aatttctgag
     1021 acttgtaatg aacagaactg acgaactccg tactgcgcgt attgagagcc tggtaacgcc
     1081 cgccgaactc gcgctacggt atcccgtaac gcctggcgtc gccacccatg tcaccgactc
     1141 ccgccgtaga attgaaaaaa tactgaatgg tgaagataag cgactgttgg tcattattgg
     1201 cccctgctcg atccacgatc tcaccgctgc gatggagtac gccacccgtc tgcagtcgct
     1261 acgcaaccag taccagccac ggctggaaat cgtaatgcgc acctattttg aaaaaccacg
     1321 aactgttgtc ggctggaaag gactcatctc cgatccagat ttaaacggta gctatcgggt
     1381 aaatcacggt ctggagctgg cgcgcaaatt acttttacag gtaaatgagc tgggcgtccc
     1441 taccgcgact gagttcctcg atatggtgac cggtcagttt attgctgatt taatcagttg
     1501 gggcgcgatt ggcgcacgta ctaccgaaag tcagatccac cgcgaaatgg cttcggcact
     1561 ctcctgtccg gtgggtttta aaaatggtac cgatggcaac acgcggattg ccgtggatgc
     1621 tatccgcgca gcccgcgcca gccatatgtt cctctcgcca gacaaaaatg gtcagatgac
     1681 catctatcag accagcggca acccgtatgg ccacattatt atgcgtggcg gcaaaaaacc
     1741 gaattatcat gccgatgata tcgccgcagc ctgcgatacg ctgcacgagt ttgatttacc
     1801 tgaacatctg gtggtggatt tcagccacgg taactgccag aagcagcacc gtcgccagtt
     1861 agaagtttgt gaggatattt gtcagcaaat ccgcaatggc tctacggcga ttgctggaat
     1921 tatggcggaa agtttcctgc gcgaaggaac gcaaaaaatc gtcggcggtc agccgctcac
     1981 ttacggtcaa tccattaccg acccgtgtct gggctgggaa gataccgaac gcctggtcga
     2041 aaaactcgcc tctgcggtag atactcgctt ctgaatgcgt gcccattcct gacgggatgg
     2101 gcatttctgc gcaacttgtt gtcttctcaa caaattactg cttgctctgg cccgc
//
LOCUS       DADSYM010000253         2152 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2415
            SAMN08039579-rid11723173.denovo.255, whole genome shotgun sequence.
ACCESSION   DADSYM010000253 DADSYM010000000
VERSION     DADSYM010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039579
            Sequence Read Archive: SRR6362533
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2152)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2152)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980162.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 03:39:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,484
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 4,347
            CDSs (with protein)               :: 4,347
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 43 of 85
            Pseudo Genes (incomplete)         :: 37 of 85
            Pseudo Genes (internal stop)      :: 19 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2152
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039579-rid11723173.denovo.255"
                     /strain="LGE2415"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8302 N 95.9628 W"
                     /collection_date="2015-08-24"
     gene            complement(1..576)
                     /locus_tag="J1Y83_004262"
                     /pseudo
     CDS             complement(1..576)
                     /locus_tag="J1Y83_004262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000382418.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
     gene            complement(576..2105)
                     /locus_tag="J1Y83_004263"
     CDS             complement(576..2105)
                     /locus_tag="J1Y83_004263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000283227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HBA7632340.1"
                     /translation="MYPIALLSVCGMMLGLGSGLASDDMAKLIPFLAIPIIKTILDFI
                     VSLGLFAFVNLPVLFAIAIPLGLLKDKEDKAYGAFSGLIGFMAMHLGTNFYLKQHDLL
                     VVADQMSTHGQTIILGIQSYNTSVLGGIVAGLLVASMYKKIVNLRIPESLGFYSGPRL
                     VPIITLIVMSGFGLIIPFIWPPFFNLFMLIGHWISTSGPVGYFFYAVAERVTIPFGLN
                     HLVTSVFRFTPIGGSAVIGGEEYYGTLNMFMAYVKENAVIPLDLAGKMEQGKLMIQYG
                     LAGAALAMYRTAHAQNRKAIKALLISGVLTVIIGGVSEPIEFLFLFVSPLLFVFHAFM
                     NGFANMVLPYMGVKMGFTGDLIQFISFGVLRGTRTGWPIAVCVEVAYFFIYYFVFRWT
                     ILKFNLMTVGREESSPVTLNAHEDTAIADIPTPDKSELQAAEQMVKALGGKENIKSLD
                     NCVTRLRLTIADMRLIDEVAIKRAGGIAVVKLDQNTLQVIIGTKVIALRRDMDNYMGI
                     Y"
BASE COUNT          667 a          511 c          406 g          568 t
ORIGIN      
        1 ccttgtacag cttgcagcga ttttatgcag ttcttcttcc gaccagactg ttcctgtcgg
       61 gttatgagga ttacataata acatcatctt tgcgctttta atcttaagat caaaatcttg
      121 ccagttgata gcgtagcccc cctcattttt tataatcaaa gaggctgtca gaatatttcg
      181 ctcgctatgt ttaattacat caaaaaatgc attatagaca ggtgtaaaaa cgagaatatt
      241 atcaccatgc gatgtcaata actctattgc ttttgcaata gagtacatca cactggggct
      301 ataggttatc cattcaggtt taatatctgt ttgatattgt tgataatacc agtttactat
      361 cgcattcaga tattcaccac attgccaacg actgtaccct aaaatcgggt gttccaggcg
      421 tttttttaat gctctggtga tagcgtctgg tatcggaaga tccatatctc aaatagagaa
      481 tggtagtaca tcttttacac caaatctgtc ctcgcaaaag tcccattggg tacaatatgt
      541 tcctttgcgg gaaacaggag tattaaaaac atccatcagt atatccccat atagttatcc
      601 atatcccgac gcagggcgat gactttagtg ccgataatga cttgtagggt attttgatca
      661 agttttacaa ccgcaatccc gccagctctt tttattgcaa cttcgtcaat caatctcata
      721 tctgcgattg ttaaacgtaa acgagttacg caattatcca gtgacttaat attctcttta
      781 ccaccaagtg ccttaaccat ctgctccgcc gcttgcagct ctgatttatc aggagttggg
      841 atatccgcta tagccgtatc ttcgtgagcg ttcagcgtaa caggactgga ctcttcacgg
      901 cctacggtca tcaggttaaa tttaaggatg gtccaacgga acacaaagta ataaatgaag
      961 aaataggcca cttcgacaca caccgcgatc ggccaacctg ttcttgtgcc acgcaatacg
     1021 ccaaagctaa taaattgaat cagatcacca gtaaatccca tcttcacccc catatatggc
     1081 agaaccatgt tagcgaatcc attcataaag gcatggaaga caaacagcag tggactgaca
     1141 aataagaaca gaaactcaat cggttcgctg acgccgccaa taatcaccgt aagcaccccg
     1201 gaaataagca atgctttgat agcctttctg ttttgagcat gagcagtgcg atacatcgcc
     1261 agcgcagcac cagccagacc atactgaatc atcagtttgc cctgctccat tttccccgcc
     1321 aaatccagcg gaatgaccgc attctctttg acgtatgcca taaacatgtt cagggtgccg
     1381 taatattctt cgccgccaat cacagccgaa ccgccgattg gcgtaaagcg gaaaactgac
     1441 gtcaccagat ggtttaagcc aaaaggaatc gtcacgcgtt cggcaactgc atagaagaaa
     1501 taaccaacag gaccggaagt tgaaatccag tggccaatga gcatgaaaag attgaaaaac
     1561 ggcggccaga taaaaggaat gatcagacca aatccactca tcacaatcag tgtaatgata
     1621 ggcaccagac gtgggccgct ataaaaacct aacgattcag gaatgcgtaa attaacgatc
     1681 tttttataca tgctggcgac taataaccca gcaacaattc cccccaacac gctggtattg
     1741 taggactgga tccccagaat gatggtttgc ccatgtgtcg acatttggtc agcaacgacc
     1801 aataagtcgt gctgtttaag ataaaagttc gttcccagat gcatcgccat aaaaccaatt
     1861 aagccagaaa aagcaccata ggctttatcc tctttatctt ttaataatcc taagggaatc
     1921 gctatcgcaa acaatacagg taaattaaca aaggcaaaca aaccaagact aacaatgaaa
     1981 tcaagtatgg ttttaattat tggaatagcc agaaatggaa ttaactttgc catatcatca
     2041 ctggctaaac cacttcccag ccctagcatc atgccacata cacttagcag agcaatggga
     2101 tacataaatg ccttccccag gctctgaaaa aaactccagg ctttcttttg tt
//
LOCUS       DADSYM010000254         2136 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2415
            SAMN08039579-rid11723173.denovo.256, whole genome shotgun sequence.
ACCESSION   DADSYM010000254 DADSYM010000000
VERSION     DADSYM010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039579
            Sequence Read Archive: SRR6362533
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2136)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2136)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980162.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 03:39:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,484
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 4,347
            CDSs (with protein)               :: 4,347
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 43 of 85
            Pseudo Genes (incomplete)         :: 37 of 85
            Pseudo Genes (internal stop)      :: 19 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2136
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039579-rid11723173.denovo.256"
                     /strain="LGE2415"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8302 N 95.9628 W"
                     /collection_date="2015-08-24"
     gene            67..450
                     /locus_tag="J1Y83_004264"
     CDS             67..450
                     /locus_tag="J1Y83_004264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA7632341.1"
                     /translation="MSVLYIQIRRNQITVRDLESKREVSGDAAFSNQRLLIANFFVAE
                     KVLQDLVLQLHPRSTWHSFLPAKRMDIVVSALEMNEGGLSQVEERILHEVVAGATLMK
                     YRQFHIHAQSVVLSDSAVLAMFKQK"
     gene            complement(514..957)
                     /locus_tag="J1Y83_004265"
     CDS             complement(514..957)
                     /locus_tag="J1Y83_004265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000236991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HBA7632342.1"
                     /translation="MVISIRRSRHEEGGELVAIWCRSVDATHDFLSAEYRAELEELVH
                     SFLPEAPLWVAVNERDQPVGFMLLSGQHMDALFIDPDVRGCGVGRMLVKHALSMAPEL
                     TTNVNEQNEQAVGFYKKVGFKVTERSEVDDLGKPYPLLNLAYVGV"
     gene            1114..2043
                     /gene="metA"
                     /locus_tag="J1Y83_004266"
     CDS             1114..2043
                     /gene="metA"
                     /locus_tag="J1Y83_004266"
                     /EC_number="2.3.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-succinyltransferase"
                     /protein_id="HBA7632343.1"
                     /translation="MPIRVPDELPAVNFLREENVFVMTTSRASGQEIRPLKVLILNLM
                     PKKIETENQFLRLLSNSPLQVDIQLLRIDSRESRNTPAEHLNNFYCNFEDIQEQNFDG
                     LIVTGAPLGLVEFNDVAYWPQIKQVLEWSKDHVTSTLFVCWAVQAALNILYGIPKQTR
                     TDKLSGVYEHHILHPHALLTRGFDDSFLAPHSRYADFPAALIRDYTDLEILAETEEGD
                     AYLFASKDKRIAFVTGHPEYDAQTLAQEYFRDVEAGLDPDVPYNYFPHNDPQNTPRAS
                     WRSHGNLLFTNWLNYYVYQITPYDLRHMNPTLD"
BASE COUNT          541 a          566 c          475 g          554 t
ORIGIN      
        1 aaaaaccccg gtcaataagg ccggggtttt ttgcatatca attatttgca tgatgaaggg
       61 aatctcatgt cagttctgta tatccaaatt cgtcgtaatc aaattactgt tcgcgatctt
      121 gaaagcaaac gtgaagtgtc aggagatgct gccttcagta accagcgcct gttaatcgcc
      181 aatttctttg ttgcagaaaa agttctgcaa gatcttgttc tgcaactcca cccacgttca
      241 acctggcatt cttttttgcc agcaaaacgt atggatattg ttgtgagcgc gctggaaatg
      301 aatgagggcg gtttgtcaca ggttgaggaa cgcattcttc atgaagtggt cgcaggggca
      361 acgttaatga aatatcgcca gttccacatc catgcgcaat cagtggtact cagtgatagt
      421 gcggtcctgg caatgtttaa gcagaaataa tcatgtcacc attggtgggt acgaaaccta
      481 aagttcagcc caccgggatg agaaaaaaac cgcctacacc cccacatatg ccagattcag
      541 caacggatac ggtttcccca aatcgtccac ctcagagcgt tccgtaacct taaagcccac
      601 cttcttatag aacccaaccg cctgctcatt ttgctcatta acattggttg tcagttccgg
      661 ggccattgag agcgcatgct tcaccagcat ccggcccacg ccgcagccgc gcacatcagg
      721 atcgataaac agcgcatcca tatgctgccc acttaacaac ataaacccaa ccggctgatc
      781 ccgctcatta accgcgaccc acaacggtgc ttccggcaga aaggaatgca ccagctcttc
      841 cagctcggcc cgatactctg ctgataaaaa atcgtgagtg gcatcgacag aacgacacca
      901 aatcgcaacg agttcccccc cctcctcatg ccgtgagcgt cgaatactaa taaccatttt
      961 ctctcctttt cgtcattctt atattctaac gtagcctttt ccttgaaact ttctcacctt
     1021 caacatgcag gctcgacatt ggtaaatttt ctggttatct tcagctatct ggatgtctaa
     1081 acgtataagc gtatgtagtg aggtaatcag gttatgccga ttcgtgtgcc ggacgagcta
     1141 cccgccgtca atttcttgcg tgaagaaaac gtctttgtga tgacaacttc tcgcgcgtct
     1201 ggtcaggaaa ttcgtccgct aaaggtgctt atccttaacc tgatgccgaa gaagatcgaa
     1261 actgaaaatc agtttctgcg cctgctttca aactcacctt tgcaggtcga tattcagctg
     1321 ttgcgcatcg attcccgtga atcgcgcaac acgcccgcag agcatctgaa taacttctac
     1381 tgtaactttg aagatattca ggaacaaaac tttgacggtc tgatagttac cggcgcgccg
     1441 ctgggcctgg tggagtttaa tgatgtcgct tactggccgc agatcaaaca ggtgctggag
     1501 tggtcgaaag atcacgtcac ctcgacgctg tttgtctgct gggctgtaca ggccgcgctc
     1561 aatatcctct acggcattcc taagcaaacc cgcaccgaca aactctctgg cgtttacgag
     1621 catcatattc tccatcctca tgcgcttctg acgcgtggct ttgatgattc attcctggca
     1681 ccgcattcgc gctatgctga ctttccggca gcgttgattc gtgattatac cgatctggaa
     1741 attctggcag agacggaaga aggggatgca tatctgtttg ccagcaaaga taagcgcatt
     1801 gcctttgtga cgggacatcc cgaatatgat gcgcaaacgc tggcgcagga atatttccgc
     1861 gatgtggaag ccgggctaga cccggatgta ccgtataact atttcccgca caatgatccg
     1921 caaaatacac cgcgagcgag ctggcgtagt cacggtaatt tgctgtttac caactggctc
     1981 aactattacg tctaccagat cacgccatat gatctacggc acatgaatcc aacgctggat
     2041 taatcttctg tgatagtcga tcgttaagcg attcagcacc ttacctcagg caccttcggg
     2101 tgcctttttt atttccgaaa cacacctcag caagtg
//
LOCUS       DADSYM010000255         2102 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2415
            SAMN08039579-rid11723173.denovo.257, whole genome shotgun sequence.
ACCESSION   DADSYM010000255 DADSYM010000000
VERSION     DADSYM010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039579
            Sequence Read Archive: SRR6362533
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2102)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2102)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980162.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 03:39:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,484
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 4,347
            CDSs (with protein)               :: 4,347
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 43 of 85
            Pseudo Genes (incomplete)         :: 37 of 85
            Pseudo Genes (internal stop)      :: 19 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2102
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039579-rid11723173.denovo.257"
                     /strain="LGE2415"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8302 N 95.9628 W"
                     /collection_date="2015-08-24"
     gene            28..1146
                     /gene="gmd"
                     /locus_tag="J1Y83_004267"
     CDS             28..1146
                     /gene="gmd"
                     /locus_tag="J1Y83_004267"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000709507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HBA7632344.1"
                     /translation="MKVALITGVTGQDGSYLAEFLLEKGYEVHGIKRRASCFNTERVD
                     HIYQDRHNENPNFFLHYGDLTDSSNLVRLIKEIQPDEVYNLGAQSHVAVSFESPEYTA
                     DVDAMGTLRLLEAIRICGLEKKTRFYQASTSELFGLVQEIPQRETTPFYPRSPYAVAK
                     MYAYWITVNYRESYGMYACNGILFNHESPRRGETFVTRKITRAIANISQGIEKCLYLG
                     NMDSLRDWGHAKDYVRMQWMMLQQDHPEDFVIATGKQISVREFVRMSAKEVGLELEFS
                     GQGVDEIATVVNKTSDCAIGVSIGDVIVRVDPRYFRPAEVETLLGDPAKAKKVLGWEP
                     EITVEEMCAEMVASDLAKAKQHALLKSHGYDVAVSLER"
     gene            1150..>2102
                     /locus_tag="J1Y83_004268"
     CDS             1150..>2102
                     /locus_tag="J1Y83_004268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000043654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-L-fucose synthase"
                     /protein_id="HBA7632345.1"
                     /translation="MTKKRIYVAGHRGMVGSAICRQLSLRDDIELVVKTHKELDLTVQ
                     KDVDAFFEQEKIDQVYLAAAKVGGIYANNTFPAEFIYQNLMIESNIIHSAHKAGIQKL
                     LFLGSSCIYPKFAEQPMNESALLTGILEPTNEPYAIAKIAGIKLCESYNRQYGRDYRS
                     VMPTNLYGINDNFHPENSHVIPALMRRFHEAKESGAPEVIVWGTGTPMREFLYVDDMA
                     AASVHVMELDEAIYQQNTQPMLSHINVGTGVDCSIREMAETMASVVGYQGKIVFDVTK
                     PDGTPRKLMDVTRLKNLGWQYQYNLHEGLSLTYKWFIENINS"
BASE COUNT          627 a          391 c          467 g          617 t
ORIGIN      
        1 atatcaaaaa tcattaatga ggtatgaatg aaagttgcat taataacagg ggttacaggt
       61 caggatggtt cttacttggc agaatttctt cttgaaaaag gttatgaagt gcatggtata
      121 aaacgccgag catcatgttt taacactgag cgtgtagacc atatctacca ggatcgccac
      181 aatgaaaatc caaatttttt cctgcactac ggtgacttaa ctgactcatc aaacttggta
      241 cgtctcatta aagaaatcca accagacgaa gtttataacc ttggcgctca gtctcatgta
      301 gcggtttcat ttgaatcacc ggaatatact gctgatgtgg atgcgatggg gactttacgt
      361 ctgctggaag cgattcgtat ttgtgggcta gagaaaaaaa cacgtttcta tcaggcatca
      421 acttctgaac tcttcggctt agttcaggaa atcccacagc gagaaacaac accattctac
      481 cctcgttcgc cttatgctgt tgcgaagatg tatgcatact ggattactgt aaactatcgt
      541 gaatcctatg gaatgtacgc ctgtaacggt attctcttca accatgaatc cccgcgtcgt
      601 ggtgaaacat tcgtaacacg taaaatcaca cgtgctattg caaatatttc gcagggaatc
      661 gaaaaatgtc tctatcttgg caatatggac tcactgcgtg actggggaca tgcgaaagat
      721 tacgtgcgta tgcagtggat gatgttgcag caagatcacc cagaagattt tgttattgca
      781 acaggcaaac aaatttccgt acgtgaattt gttcgtatgt cagctaaaga agtaggtctg
      841 gagttagaat tctcaggaca aggtgttgat gaaatagcaa ctgttgtcaa taaaacatct
      901 gactgtgcta ttggtgttag tattggggat gtgatcgttc gtgttgatcc tcgttatttc
      961 cgtcctgcag aagttgaaac tcttcttggt gatccagcaa aagcgaaaaa agtattgggt
     1021 tgggaaccag agattacagt tgaagagatg tgtgcagaaa tggttgccag tgatctggcg
     1081 aaagcaaaac agcatgcact actgaaaagc catggttacg atgttgcagt ttctctggag
     1141 cggtaaggta tgacaaaaaa acgtatctac gttgctggtc accgaggtat ggttggctct
     1201 gctatttgcc gtcaattatc actgcgtgat gatatcgaat tagtggtcaa aacacacaaa
     1261 gaactcgatc taaccgtaca gaaggatgtt gatgcatttt ttgagcaaga gaaaattgat
     1321 caggtttatc ttgctgcggc taaagttggt ggcatttatg ccaacaatac atttccggca
     1381 gaattcatct atcagaatct catgattgag agtaatatta ttcattcagc tcacaaggcc
     1441 ggaattcaaa aattactttt tttaggctca agctgtattt atcctaaatt cgcagagcag
     1501 cctatgaacg agtctgcact tttaactggc atacttgagc caactaatga accatacgca
     1561 attgctaaaa ttgcgggaat aaaattatgt gaatcttata accgtcaata tggccgtgat
     1621 tatcgcagtg taatgcctac taatctttat ggcataaatg ataatttcca tcctgaaaac
     1681 tctcatgtta ttccggcact catgcgtaga ttccatgaag caaaagagag tggtgcgcca
     1741 gaggttattg tctggggaac cggaacaccg atgcgtgagt ttttatatgt tgatgatatg
     1801 gctgccgcat ctgtccatgt aatggagctt gacgaagcaa tttatcaaca aaatacacag
     1861 cctatgttat cccatattaa tgttggtacg ggtgtggatt gttctatacg tgaaatggct
     1921 gaaacaatgg cctctgtggt ggggtatcaa ggtaaaattg tttttgatgt tactaaacct
     1981 gatggcactc cgcgtaaact tatggacgtt acccggctca aaaacttggg ctggcaatat
     2041 caatataatt tacatgaagg cttatcatta acatataaat ggtttattga gaatattaat
     2101 tc
//
LOCUS       DADSYM010000256         2072 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2415
            SAMN08039579-rid11723173.denovo.258, whole genome shotgun sequence.
ACCESSION   DADSYM010000256 DADSYM010000000
VERSION     DADSYM010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039579
            Sequence Read Archive: SRR6362533
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2072)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2072)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980162.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 03:39:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,484
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 4,347
            CDSs (with protein)               :: 4,347
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 43 of 85
            Pseudo Genes (incomplete)         :: 37 of 85
            Pseudo Genes (internal stop)      :: 19 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2072
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039579-rid11723173.denovo.258"
                     /strain="LGE2415"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8302 N 95.9628 W"
                     /collection_date="2015-08-24"
     gene            <1..1285
                     /locus_tag="J1Y83_004269"
     CDS             <1..1285
                     /locus_tag="J1Y83_004269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBA7632346.1"
                     /translation="DGTGKPVQNCTIQLKAKRNSTTVVVNTVASENPDEAGRYSMDVE
                     YGQYSVILLVEGFPPSHAGTITVYEDSQPGTLNDFLGAMSEDDVRPEALRRFELMVEE
                     AARHAEEAKKNAGEAETSARNAGISASQAEESAANADTSAGDASESARQAAESAASAK
                     QSEDASSSSASAAAQKASESLQSATDAELSKKMAESAAGNAARDATTATEKARESAES
                     AQSAEQSRIAAEEAVNRIPTVVGPPGPKGEQGPAGPQGPKGDKGERGDTGPVGATGER
                     GPAGDAGPAGPQGPKGDRGERGETGLTGNAGPQGPKGDTGAAGPAGPQGPKGETGAAG
                     PVGATGPQGPKGDPGETQIRFRLGPASIIETNSNGWFPDTDGALITGLTFLDPKDATQ
                     VQGLFQHLQVRFGDGPWQDVKGLNEVGSDTGRTGE"
     gene            1287..1556
                     /locus_tag="J1Y83_004270"
     CDS             1287..1556
                     /locus_tag="J1Y83_004270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310967.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBA7632347.1"
                     /translation="MNILKKLMQRLCGCGKHDGREHVQSLTAQLRLGPADILESDENG
                     IIPEQDRVITQVVILDADKKQIQCVVRPLQILRADGTWENIGGMK"
     gene            1733..>2072
                     /gene="nleB2"
                     /locus_tag="J1Y83_004271"
     CDS             1733..>2072
                     /gene="nleB2"
                     /locus_tag="J1Y83_004271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector arginine
                     glycosyltransferase NleB2"
                     /protein_id="HBA7632348.1"
                     /translation="MLSPIRTTFHNSANIVQSSPCQTVSFAGKEYELKVIDEKTPILF
                     QWFEPNPERYKKDEVPIVNTKQHPYLDNVTNAARIESDRMIGIFVDGDFSVNQKTAFS
                     KLERDFENVMI"
BASE COUNT          581 a          447 c          654 g          390 t
ORIGIN      
        1 agacggcaca ggaaaaccgg tgcagaactg cacaatccag ctgaaagcaa aacgtaacag
       61 taccacggtg gtggtgaaca cggtggcctc agaaaatccg gatgaagccg ggcgttacag
      121 catggacgtt gagtacggtc agtacagcgt cattctgttg gtggaaggat tcccgccgtc
      181 acatgccggg accatcaccg tgtatgaaga ttctcaaccg gggacgctga atgattttct
      241 cggtgccatg tcggaggatg acgtccggcc ggaggcactg cgtcgttttg aactgatggt
      301 ggaagaagcg gcgcgtcacg ctgaggaggc gaagaagaat gccggagagg cggagacgtc
      361 cgcgaggaat gctggcatat cagccagtca ggcagaagag agcgctgcaa atgctgacac
      421 ttcagcaggg gatgcatcgg agtcagcccg gcaggcggca gaaagtgcag cctcagcaaa
      481 gcagtcagag gatgcgtcct cgtcctcggc ctctgcggcc gctcaaaaag ccagtgagtc
      541 attacaaagt gcaacagatg ctgagttgtc aaaaaagatg gcagaaagtg cagccggtaa
      601 tgcagccagg gatgcaacga ccgcaacaga aaaagcccgg gagtcagcag aaagcgcaca
      661 gtcagcggaa caaagcagga tagcggcgga agaggccgta aaccgaatcc ccaccgtggt
      721 gggacctccc gggccaaagg gggaacaggg gcccgcgggt cctcaggggc cgaagggtga
      781 taagggagag cgcggtgaca ccggccctgt cggggcaacc ggcgaacggg gaccggcagg
      841 tgatgctggt ccggcaggcc cgcaggggcc gaaaggtgac aggggagagc ggggagagac
      901 cggtctgacg ggaaatgcag gtccacaggg tccaaaggga gataccggtg cggcaggccc
      961 ggcaggccca cagggaccga aaggagaaac aggtgcggct ggcccggtgg gggcaaccgg
     1021 acctcaggga ccgaagggcg acccggggga gacacaaatc cgttttcgtc tggggccggc
     1081 gagcattatt gagacaaaca gcaatggctg gttcccggat acagatggcg cactcatcac
     1141 cggactgacc tttcttgacc ccaaagatgc cacacaggtt caggggctgt ttcagcattt
     1201 gcaggtcagg tttggtgacg ggccgtggca ggatgttaag gggctgaatg aagtgggcag
     1261 tgatacaggc agaacaggag aatgacatga atatactaaa aaaacttatg cagcgtctgt
     1321 gtggttgcgg aaagcatgat ggccgtgaac acgtgcagtc gcttacagca caactgcgac
     1381 tgggaccggc agacattctg gagtcagatg agaatggcat tattccggag caggacaggg
     1441 taatcacaca ggtggtgata ctggatgcgg ataaaaagca gatacagtgc gtggtaagac
     1501 cgctgcaaat tctgcgtgct gacgggacgt gggaaaatat tggcgggatg aagtaacccg
     1561 acagcttcac aaaaccggag tccggctccg gtttttgttg gcatgccatg gtgatgtttg
     1621 ttaggaaagc aaaggtggca aaactgctgg aggttttgtg gttgagtatg ccaatataat
     1681 taatagatta aagagttagt tgtgaagaaa atatggataa acaggacgac gaatgctttc
     1741 accgataagg acaactttcc ataactcagc aaatatagtg cagagttcac cctgtcaaac
     1801 ggtttctttt gccggaaagg aatatgagtt aaaggtcatt gatgaaaaaa cgcctattct
     1861 ttttcagtgg tttgaaccta atcctgaacg atataagaaa gatgaggttc caatagttaa
     1921 tactaagcag catccctatt tagataatgt cacaaatgcg gcaaggatag agagtgatcg
     1981 tatgataggt atttttgttg atggcgattt ttcagtcaac caaaagactg ctttttcaaa
     2041 attggaacga gattttgaaa atgtaatgat aa
//
LOCUS       DADSYM010000257         2051 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2415
            SAMN08039579-rid11723173.denovo.259, whole genome shotgun sequence.
ACCESSION   DADSYM010000257 DADSYM010000000
VERSION     DADSYM010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039579
            Sequence Read Archive: SRR6362533
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2051)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2051)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980162.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 03:39:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,484
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 4,347
            CDSs (with protein)               :: 4,347
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 43 of 85
            Pseudo Genes (incomplete)         :: 37 of 85
            Pseudo Genes (internal stop)      :: 19 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2051
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039579-rid11723173.denovo.259"
                     /strain="LGE2415"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8302 N 95.9628 W"
                     /collection_date="2015-08-24"
     gene            <1..1376
                     /gene="escC"
                     /locus_tag="J1Y83_004272"
     CDS             <1..1376
                     /gene="escC"
                     /locus_tag="J1Y83_004272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type III secretion system LEE outer membrane
                     ring protein EscC"
                     /protein_id="HBA7632349.1"
                     /translation="PVFISSSVNDDFSGEIKNEKPVKVLEKLSKLYHLTWYYDENILY
                     IYKTNEISRSIITPTYLDIDSLLKYLSDTISVNKNSCNVRKITTFNSIEVRGVPECIK
                     YITSLSESLDKEAQSKAKNKDVVKVFKLNYASATDITYKYRDQNVVVPGVVSILKTMA
                     SNGSLPSTGKGAVERSGNLFDNSVTISADPRLNAVVVKDREITMDIYQQLISELDIEQ
                     RQIEISVSIIDVDANDLQQLGVNWSGTLNAGQGTIAFNSSTAQANISSSVISNASNFM
                     IRVNALQQNSKAKILSQPSIITLNNMQAILDKNVTFYTKVSGEKVASLESITSGTLLR
                     VTPRILDDSSNSLTGKRRERVRLLLDIQDGNQSTNQSNAQDASSTLPEVQNSEMTTEA
                     TLSAGESLLLGGFIQDKESSSKDGIPLLSDIPVIGSLFSSTVKQKHSVVRLFLIKATP
                     IKSASSE"
     gene            1390..1845
                     /gene="cesD"
                     /locus_tag="J1Y83_004273"
     CDS             1390..1845
                     /gene="cesD"
                     /locus_tag="J1Y83_004273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE chaperone CesD"
                     /protein_id="HBA7632350.1"
                     /translation="MSRKFSSLEDIYDFYQDGGTLASLTNLTQQDLNDLHSYAYTAYQ
                     SGDVITARNLFHLLTYLEHWNYDYTLSLGLCHQRLSNHEDAQLCFARCATLVMQDPRA
                     SYYSGISYLLVGNKKMAKKAFKACLMWCNEKEKYTTYKENIKKLLGNTE"
BASE COUNT          691 a          381 c          390 g          589 t
ORIGIN      
        1 ttcctgtttt catcagtagt tccgtgaatg atgatttctc tggcgaaata aaaaatgaaa
       61 aacctgtaaa agtactggaa aaactgtcca aactctatca tctcacatgg tattatgatg
      121 agaacatctt gtatatttat aaaacaaatg aaatatcaag aagtataatt acaccaacat
      181 atctggatat tgactcactg ctaaaatatc tgagtgatac aatctcagta aacaaaaact
      241 cttgcaatgt gagaaaaatt acgacattta actcgattga agttcggggt gttcctgaat
      301 gtataaaata tattacatct ttatcagaaa gtcttgataa agaggcacag tctaaagcca
      361 aaaataaaga tgttgttaag gtctttaaac tgaactatgc gtcagcgaca gatataacat
      421 acaaataccg tgaccaaaat gttgtcgtcc caggtgttgt ctccattctg aagacaatgg
      481 caagtaatgg ttcgcttccc agtaccggta aaggtgccgt cgagcgaagc ggtaacctgt
      541 ttgataacag tgtaacgatt tccgctgatc cacgtcttaa tgcagttgtt gtaaaagacc
      601 gcgaaataac gatggatatc tatcagcaat taatttctga gcttgatatt gaacaaaggc
      661 aaattgaaat ttctgtctca attattgatg ttgatgcaaa cgatcttcag cagctcggcg
      721 taaactggtc cggtacgtta aatgcaggac aaggtacgat tgcctttaat agctctaccg
      781 cacaggctaa tatcagctct tctgtgataa gtaatgccag taattttatg attagggtga
      841 atgcgttaca acagaactcg aaagctaaaa tactctctca gccgtcaatt ataaccttaa
      901 ataatatgca ggcgattctt gataaaaacg taacgtttta tacaaaagtc tccggagaaa
      961 aggtggcgtc tctggaaagt attacaagcg gaacattgct gagagtgaca ccacgtatac
     1021 ttgatgacag ctcgaatagt cttaccggaa aacgtcggga acgagttcgt ttgttacttg
     1081 atattcagga tggcaatcaa tcgactaacc aaagcaatgc tcaggatgcc agcagcacct
     1141 tacctgaagt acaaaattca gaaatgacaa cagaagcgac attaagtgca ggagaaagtt
     1201 tattacttgg cggattcatc caagataaag aaagttcaag taaagatggt ataccgctgt
     1261 taagcgacat tcctgttatt ggtagtttat tctccagtac ggtaaaacaa aaacatagtg
     1321 ttgttcgact ctttttaatt aaagcaaccc cgataaaatc tgcatctagc gaataataaa
     1381 tgaggcttta tgagcaggaa atttagctct ctagaggata tttatgattt ctaccaggat
     1441 ggtggcacat tagcgtcatt aacaaatctg acacaacaag atctcaatga ccttcattct
     1501 tatgcctata cagcatatca atctggtgat gttataaccg caagaaatct attccatttg
     1561 ctcacatatc tggaacactg gaattatgac tacaccttat ctctgggctt atgccatcag
     1621 cgtttatcaa atcatgaaga tgcacaactg tgtttcgcac gctgtgcaac tttagttatg
     1681 caagatccca gggcatctta ttattctgga attagctact tactcgtcgg aaataagaaa
     1741 atggccaaga aagcctttaa ggcttgttta atgtggtgta atgaaaaaga aaaatacact
     1801 acatataaag aaaatattaa aaaattgtta ggtaatacag agtaaaaact accatcagct
     1861 ataccaatcc ccaggctgac tggggatttt ctatcggaaa taataaagct gctatagtga
     1921 agtgctccca aagttgagca ccgaccactg gtagatttat ctacaggtgt ggggttattc
     1981 cattaaccag gattttatgc cctgaactat cttctgctgt atttaatatc atttccgttt
     2041 atgaaacaat c
//
LOCUS       DADSYM010000258         2045 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2415
            SAMN08039579-rid11723173.denovo.260, whole genome shotgun sequence.
ACCESSION   DADSYM010000258 DADSYM010000000
VERSION     DADSYM010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039579
            Sequence Read Archive: SRR6362533
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2045)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2045)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980162.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 03:39:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,484
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 4,347
            CDSs (with protein)               :: 4,347
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 43 of 85
            Pseudo Genes (incomplete)         :: 37 of 85
            Pseudo Genes (internal stop)      :: 19 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2045
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039579-rid11723173.denovo.260"
                     /strain="LGE2415"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8302 N 95.9628 W"
                     /collection_date="2015-08-24"
     gene            complement(92..277)
                     /locus_tag="J1Y83_004274"
     CDS             complement(92..277)
                     /locus_tag="J1Y83_004274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000203370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA7632351.1"
                     /translation="MTSNQSENEIVNTVKKYASCADDALQGGKENVKDKFRNYPLTSV
                     VVALSIGLTLGFIIGRK"
     gene            complement(903..992)
                     /locus_tag="J1Y83_004275"
     CDS             complement(903..992)
                     /locus_tag="J1Y83_004275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA7632352.1"
                     /translation="MNNPVCLDDWLIGFKSLCCTLAVIALLII"
     gene            complement(1047..1259)
                     /gene="cspF"
                     /locus_tag="J1Y83_004276"
     CDS             complement(1047..1259)
                     /gene="cspF"
                     /locus_tag="J1Y83_004276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock-like protein CspF"
                     /protein_id="HBA7632353.1"
                     /translation="MSRKMTGIVKTFDGKSGKGLITPSDGRIDVQLHVSALNLRDAEE
                     ITTGLRVEFCRINGLRGPSAANVYLS"
     gene            1560..1775
                     /gene="cspB"
                     /locus_tag="J1Y83_004277"
     CDS             1560..1775
                     /gene="cspB"
                     /locus_tag="J1Y83_004277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock-like protein CspB"
                     /protein_id="HBA7632354.1"
                     /translation="MSNKMTGLVKWFNADKGFGFISPVDGSKDVFVHFSAIQNDNYRT
                     LFEGQKVTFSIESGAKGPAAANVIITD"
BASE COUNT          619 a          353 c          441 g          632 t
ORIGIN      
        1 atccttaaac cacctccaca ggaggtggtt ttttactaat ggaacattaa ttggcagatg
       61 tgattgtgta cgacttatgg gaggcttaag attattttct gccaataata aaaccaagcg
      121 ttaaccctat gcttagagca accaccacgc tagtcagtgg ataatttcta aatttatctt
      181 taacgttttc tttgcctccc tgcaaagcat cgtcagcaca tgatgcatat tttttgacag
      241 tattaactat ttcgttttca gattgatttg aagtcatgtt tgaagtcctg atgttaagtg
      301 gctttttaat tatagcagat atgctttcaa tgatagggcg gagagttgtt attgaggtga
      361 tactatgacc actcaatcaa cattacataa aatgacgttt actcctgttt ggcacagtag
      421 cagaattttg cttctgctac tgaagtcgct ttagcactag aatatgcaga tggataaagc
      481 agttcagcgt atgtactgcg tcatgcatat cggtcttgca gatatgacga gaaggggaga
      541 caaggtgtgt gtaatgtaga agtctttaaa gtgctgtctg ggataactct atgctgaagt
      601 gcaattgata aaagtaacac gataaaggtt tttcaatgaa gatggtaacg tgatgcttgc
      661 atttttcaaa aaatatggat aaaatttttt caacgatggg ctttgtatac ccgacattaa
      721 gaaaaagtag aaaacccgct gatgagcggg ttttgtgctt taaatggggc aatggtaatg
      781 ttgaatctca tcccgggact catgtctgtt aacttattat ttagctggtg acttggttat
      841 ttgcctgatg tttaaaatgt tttcttccag tacaatgtcc ctaaacacaa tgagtctgct
      901 tattatatta ttagcagagc tattacggcc aaagtacagc ataagctttt aaagccaatc
      961 aaccagtcat caagacagac ggggttattc ataaaaactc tccatgtgtg atccgatggg
     1021 gcctgaaatt aaagctttaa tatagctcat gaaaggtaaa cattggcagc tgaagggcca
     1081 cgcagaccat ttatccggca aaattccacg cgtaatccgg tggtaatttc ttctgcatcg
     1141 cggagattga gcgctgaaac atgaagctgg acatcgatac gaccatcgga tggggtgata
     1201 agacccttgc cgcttttgcc gtcaaaggtt ttgacaattc ctgtcatttt acgggacaaa
     1261 aaaattcctt aatactgata acttggcgca ctatacacac gttcctgaag aaagctatag
     1321 ttttttgatg gggttgaaga tggctggatg tctaaaataa acattgcttc atatgttcaa
     1381 ctatgcgtta atgattgcgt cggtttgaag aacagacgat atacgaagta gtttactaaa
     1441 gcagttctca tttcaggtgt tattcactta ttccttcttt gagtctctcc aattaagtac
     1501 gaagtcgttt ctgttatgca aaccatttat gccgaaaggc tcaagttaag gaatgtagaa
     1561 tgtcaaataa aatgactggt ttagtaaaat ggtttaacgc tgataaaggt ttcggcttta
     1621 tttctcctgt tgatggtagt aaagatgtgt ttgtgcattt ttctgcgatt cagaatgata
     1681 attatcgaac cttatttgaa ggtcaaaagg ttaccttctc tatagagagt ggtgctaaag
     1741 gtcctgcagc agcaaatgtc atcattactg attaaaattc atcgctcgtc tgtatacgat
     1801 aacgaagaag gctgatgcct gagtagagat acggacagag tagtgaatat tggatctctt
     1861 taataaaaag taaggaggtc caatacatga aacaatggcc agcatatttg gcaaaatctt
     1921 aatcaggaaa agtatgctaa ccattgtggt gaagtgcagg tttgctgcat gaatagtttc
     1981 acagcagaag ctaactgctg gcatcgcaaa acaaagtgcg taagtggatg actcccacaa
     2041 aaagc
//
LOCUS       DADSYM010000259         2042 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2415
            SAMN08039579-rid11723173.denovo.261, whole genome shotgun sequence.
ACCESSION   DADSYM010000259 DADSYM010000000
VERSION     DADSYM010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039579
            Sequence Read Archive: SRR6362533
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2042)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2042)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980162.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 03:39:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,484
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 4,347
            CDSs (with protein)               :: 4,347
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 43 of 85
            Pseudo Genes (incomplete)         :: 37 of 85
            Pseudo Genes (internal stop)      :: 19 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2042
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039579-rid11723173.denovo.261"
                     /strain="LGE2415"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8302 N 95.9628 W"
                     /collection_date="2015-08-24"
     gene            complement(373..990)
                     /gene="tdk"
                     /locus_tag="J1Y83_004278"
     CDS             complement(373..990)
                     /gene="tdk"
                     /locus_tag="J1Y83_004278"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HBA7632355.1"
                     /translation="MAQLYFYYSAMNAGKSTALLQSSYNYQERGMRTVVYTAEIDDRF
                     GAGKVSSRIGLSSPAKLFNQNSSLFDEIRAEHEQQAIHCVLVDECQFLTRQQVYELSE
                     VVDQLDIPVLCYGLRTDFRGELFIGSQYLLAWSDKLVELKTICFCGRKASMVLRLDQA
                     GRPYNEGEQVVIGGNERYVSVCRKHYKEALEVGSLTAIQERHRHD"
     gene            1594..2007
                     /gene="hns"
                     /locus_tag="J1Y83_004279"
     CDS             1594..2007
                     /gene="hns"
                     /locus_tag="J1Y83_004279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator H-NS"
                     /protein_id="HBA7632356.1"
                     /translation="MSEALKILNNIRTLRAQARECTLETLEEMLEKLEVVVNERREEE
                     SAAAAEVEERTRKLQQYREMLIADGIDPNELLNSLAAVKSGTKAKRAQRPAKYSYVDE
                     NGETKTWTGQGRTPAVIKKAMDEQGKSLDDFLIKQ"
BASE COUNT          611 a          470 c          414 g          547 t
ORIGIN      
        1 aataactggc tcaaagctga aagctttccg gaacccccag cctagctggg ggttttctgt
       61 gcacaaaaag agccagtcag ttgcttaact gactggcatt accccttctg gggccgtttt
      121 ttgttttccc aaaacaactt ttccataaac cggcagcatt gtccttcatc atatttgcga
      181 cgatgtataa cgcctacaca cagcgatatt gtactttaca ggttacaagt caatgtcggt
      241 gtataagcgc tctgtgaggc gtatagcggc gttaaaaaac tgccgagaag ggtgtatagc
      301 ccgaaagaag tgcgtaaaac gaactgacag gataaaagcg ccctgctcac cctgtcagta
      361 aagaaattct tattaatcgt ggcgatgcct ttcctgaata gccgttaatg agccgacttc
      421 taacgcttct ttatagtgtt tacggcatac agaaacgtag cgttcattac caccaataac
      481 cacctgctca ccttcgttat aaggtctgcc tgcttgatca agacgcagca ccatgcttgc
      541 tttacggcca caaaaacaga tggtttttaa ttcaaccagt ttgtcggacc acgccagtaa
      601 gtattgactg ccaataaata attcacctcg aaaatcggta cgtaaaccat aacaaagtac
      661 aggtatatcg agttgatcga caacctccga taattcatat acttgttgtc tggttaaaaa
      721 ctggcattca tcaaccagta cgcaatgaat tgcctgctgt tcatgttccg cacgaatctc
      781 atcaaataat gatgaatttt ggttaaataa ttttgcaggc gatgacaaac ctatacgcga
      841 actgactttc ccggcaccaa agcgatcatc aatttctgcc gtatatacga cagtgcgcat
      901 gccgcgttcc tggtaattgt atgaagattg caacaatgct gtagacttac ccgcattcat
      961 tgcggaatag tagaaatata gctgtgccat cagccacagg ccctcaatga tatgaaataa
     1021 agttggctgg agtttatcat aattcgtcag tttttcagct caacagtatg cacagaagtg
     1081 gtatatgcgt tctcccttac gaagccttgc ataatccttc tgagctatca ttacaactgc
     1141 ctcgcttgtt ataagcgggt aaatgactgc cggtaactat tcacaatctt taacctgttg
     1201 cgcatgtaat agccctctgt tgacctccag gagatagtgc aatactaagt ccctgctctt
     1261 attgcgactg ttctactttt catcattcgc ttaataggga attctcgtaa acacggctaa
     1321 tacagaagac tgaaaggtcg tcagcctacg attatctccc cataaaatgt gacatgaatc
     1381 aggaagtttt aacctcacgt gctgcgaaat catcggtgca aatagggcta tatgccgcgt
     1441 cttttctggc taattttatg aaaagatatt tattggcggc acaaaataaa gaacaatttt
     1501 gaattcctta cattcctggc tattgcacaa ctgaatttaa ggctctatta ttacctcaac
     1561 aaaccacccc aatataagtt tgagattact acaatgagcg aagcacttaa aattctgaac
     1621 aacatccgta ctcttcgtgc gcaggcaaga gaatgtacac ttgaaacgct ggaagaaatg
     1681 ctggaaaaat tagaagttgt tgttaacgaa cgtcgcgaag aagaaagcgc ggctgctgct
     1741 gaagttgaag agcgcactcg taaactgcag caatatcgcg aaatgctgat cgctgacggt
     1801 attgacccga acgaactgct gaatagcctt gctgccgtta aatctggcac caaagctaag
     1861 cgtgctcagc gtccggcaaa atatagctac gttgacgaaa acggcgaaac taaaacctgg
     1921 actggccagg gccgtactcc agctgtaatc aaaaaagcaa tggatgagca aggtaaatcc
     1981 ctcgacgatt tcctgatcaa gcaataatct tttgtagatt gcacatgcta aaaatcccgc
     2041 ca
//
LOCUS       DADSYM010000260         1997 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2415
            SAMN08039579-rid11723173.denovo.262, whole genome shotgun sequence.
ACCESSION   DADSYM010000260 DADSYM010000000
VERSION     DADSYM010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039579
            Sequence Read Archive: SRR6362533
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1997)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1997)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980162.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 03:39:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,484
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 4,347
            CDSs (with protein)               :: 4,347
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 43 of 85
            Pseudo Genes (incomplete)         :: 37 of 85
            Pseudo Genes (internal stop)      :: 19 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1997
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039579-rid11723173.denovo.262"
                     /strain="LGE2415"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8302 N 95.9628 W"
                     /collection_date="2015-08-24"
     gene            49..297
                     /locus_tag="J1Y83_004280"
     CDS             49..297
                     /locus_tag="J1Y83_004280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA7632357.1"
                     /translation="MNNSIPDRFIFQCALFKNLEREVFMTHGYVDSHIIAQALRLRLK
                     DETSVILSDLYLQILQHIEMHKTTLTDIIINDRESVLS"
     gene            595..1488
                     /locus_tag="J1Y83_004281"
                     /pseudo
     CDS             595..1488
                     /locus_tag="J1Y83_004281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415686.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
     gene            1620..1838
                     /locus_tag="J1Y83_004282"
     CDS             1620..1838
                     /locus_tag="J1Y83_004282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001065870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA7632358.1"
                     /translation="MNNSNNLDYFTLYIIFSIAFMLITLLVILIAKPSTGLGEVLVTI
                     NLLNALVWLAINLVNRLRERLVSHRDQH"
BASE COUNT          536 a          394 c          435 g          632 t
ORIGIN      
        1 actatacaca gcataatttt attggatcac tattcctgcg aggccgacat gaataattca
       61 atcccagaca gatttatttt tcaatgtgct ttgtttaaaa atctcgagag ggaggtgttc
      121 atgacacatg gatatgttga tagtcatatt attgctcagg ctttacgctt gcgtttaaaa
      181 gatgagacca gtgttattct ttctgatctc tatctgcaaa tcttgcagca cattgaaatg
      241 cataagacta cactaacgga tatcattatt aatgacaggg aatccgtgct ctcttaaaac
      301 ttgagtaagg tttttgaata aacatcagct gtatcactat gctgatgcaa agtgagcatt
      361 tcaggcgtta tgctttctta atatatccgc aatatcagag ggcaaacagg aatggagagc
      421 gctcgttttc cattcttgac gcctgatatc ccgcctaatt tgtttaattc tgcctacatt
      481 gtgctaaaaa attaataggc tagatgaagt tggagaaaat tttatcttcg gcgtggatat
      541 ttgtgatgct ctctaccatg ctctctgtag accccagcgc gagcgaatga gcgtctcttt
      601 agcttctaca gacttatcga taaaggcttt gagattttga tatttattct gttaatcgcc
      661 tgcgctgctg ccttcctgct tgataggtat ttcaataaaa gcgcaacgcc tgaagagatc
      721 ctgcgacggg ctataaataa tggggagatc gtcccttttt accaacctgt ggtaaatggt
      781 cgggaaggga cattgcgggg agttgaggtg ttagcacgct ggaaacaacc tcacggtgga
      841 tatatatcac ccgcggcatt tattccactt gctgaaaaat gcggattaat cgttccgctt
      901 acgcaaagcc tgatgaatca ggttgccaga cagatgaacg ctatcgcgag taaactgccg
      961 gaaggttttc atattgggat taattttagc gcctcgcata ttatttcgcc gacgtttgtc
     1021 gacgagtgct taaattaccg tgacagtttt acccgccgcg atttaaacct tgttctggaa
     1081 gtcaccgagc gtgagccatt aaatgttgat gaaagtctgg ttcagcggtt gaacattctg
     1141 catgaaaatg gttttgtcat cgcgctggat gatttcggta ctggttactc agggctttct
     1201 tatctgcatg acctgcatat tgattatatc aaaattgatc acagtttcgt tggccgcgtc
     1261 aacgcagacc cagcatcaac ccgaattctg gattgtgtat tggatctggc gcgtaaactt
     1321 tcgatcagta tcgtcgctga aggtgtcgaa acgaaagaac aatttgacta tctgaaccaa
     1381 aataatatca catttcagca gggttattat ttctataaac ctgttacata catcgacctg
     1441 gtcaaaatta tcctttctaa accgaaggtg aaggttgtgg ttgagtgaaa attgatcagc
     1501 aaggccatgg tgcggtgtaa ttatagacag ctaattagct cgttgcctct tgttactatt
     1561 gttcattctt ttgtttgcta taattgtgtg aaagttttga ctggattgcc attagtagca
     1621 tgaacaatag taataatctg gattatttca ctctctatat catcttttcc attgcattta
     1681 tgctgatcac tctcctggtc atccttattg caaaacccag caccgggctg ggagaagtgc
     1741 ttgtgacgat aaatttgctt aatgcccttg tttggctggc gatcaatctg gttaatcgat
     1801 taagggaaag actcgtcagc cacagggatc agcactaatc tttcagtttc tcactgccag
     1861 tatgcggctg aatgggttgc tggcagtgaa cccctggatc attgaaggaa aggaattatt
     1921 gcacaaatag ttgtcaaccc tggtgttatc acagttgttt tatatatcag tgaaataatt
     1981 tctatggcaa ctgttga
//
LOCUS       DADSYM010000261         1974 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2415
            SAMN08039579-rid11723173.denovo.263, whole genome shotgun sequence.
ACCESSION   DADSYM010000261 DADSYM010000000
VERSION     DADSYM010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039579
            Sequence Read Archive: SRR6362533
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1974)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1974)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980162.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 03:39:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,484
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 4,347
            CDSs (with protein)               :: 4,347
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 43 of 85
            Pseudo Genes (incomplete)         :: 37 of 85
            Pseudo Genes (internal stop)      :: 19 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1974
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039579-rid11723173.denovo.263"
                     /strain="LGE2415"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8302 N 95.9628 W"
                     /collection_date="2015-08-24"
     gene            196..>1974
                     /gene="tssI"
                     /locus_tag="J1Y83_004283"
     CDS             196..>1974
                     /gene="tssI"
                     /locus_tag="J1Y83_004283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000006341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tip protein VgrG"
                     /protein_id="HBA7632359.1"
                     /translation="MSFAATDYPSGALAGSGTHNVTLSGSAVPDGMLLFASLGGTETV
                     GELFTYSIKLKTPDILNLDYVSPAANLQLKPMVGKDLCVNIELDGGGKRYISGLVTAA
                     RVAGHQGRSVVYELRLEPWLKTLTHTSDYKAFQNKNVVNILDEVLDEYPWPVEKRLVE
                     NYPTRAWQVQYGETDFDFIQRLMQEWGIYWWFEHNENSHTLVLADAINVHKACADSPL
                     VCYYQKGLKLDKEFIHTITANESLRSGQWVLNDFDFMKPRSLLKSTVANPRETGLAEY
                     EHYEWPGDYFTKSEGEMLTRIRMEEQRSPGSRVQGSGNIRTLMTGFTFTLENYPTAEV
                     NREYLLVQTTLFIQDNAQHSGQDQHFTYSTSFELHPTGEVYRPQRTLSKPHTKGPQSA
                     IVTGPAGQEIWTDKYGRVKVQFGRDRYGKNDENSSCWVRVSYPWAGKGFGGIQIPRIG
                     QEVLVDFKNGDPDLPIIVGRTYNQDTMPPWGLPGAATQSGFYSHTIGGGPTNANALRF
                     EDKPGGEEVWLHAEKDQRIEVNNNESHWVGNNRLKVIDKTETAIIGEKRSLTVQMDDT
                     SLAGQNKTIQAVQNLRLAAGDSIILSC"
BASE COUNT          552 a          447 c          512 g          463 t
ORIGIN      
        1 ttaatgtact tgagatgaat attttcacca gattagcatg acggtgcatt cttcaaggca
       61 tatatggtta atagtatctt cagtagcatg cataagtatt gagatgtttt atgccagcta
      121 cgaatatatt gcatatctca ttctctgtaa gatttcttat tagcacttag ccatttctag
      181 ttaaaaggat taattatgag ttttgcagct actgattatc catccggcgc actagctgga
      241 tcgggtacac ataatgtgac tttgagcggc agcgcagttc ctgatggaat gttactgttt
      301 gcaagcttag gggggacaga aacagtaggg gaactattta cttattctat taaattaaaa
      361 actcctgaca tcctgaacct ggactatgtt tcccccgcag ccaacctgca gctcaaaccg
      421 atggtgggca aagatctgtg cgtcaatatc gaattggatg gcggcggaaa acgttatatc
      481 agcgggctgg tgacggcggc acgcgtggcc ggtcatcaag gacgctcggt ggtgtatgaa
      541 ctgcgactcg aaccgtggtt aaaaacactg acccacacta gtgactacaa agcgtttcag
      601 aataaaaacg tggtgaacat cctggatgag gtactggatg agtacccctg gccagtggaa
      661 aaacgattgg tagaaaacta tccgactcgc gcctggcagg tgcagtacgg tgaaacagat
      721 ttcgatttta ttcagcgcct gatgcaggag tggggtatct actggtggtt tgagcacaac
      781 gagaacagtc acacgttagt gctggctgat gccattaatg tccacaaggc atgcgcagat
      841 tcgccgctgg tgtgttatta ccagaaaggg ctgaaactgg acaaggagtt tatccatacc
      901 atcacagcaa atgaaagcct gcgcagcgga cagtgggtgc tgaatgattt cgactttatg
      961 aagccgcgtt cactgctgaa gagtactgtg gctaatccgc gtgaaaccgg ccttgctgaa
     1021 tatgagcact acgaatggcc gggagactac ttcacaaaga gtgaaggtga aatgctgaca
     1081 cgcatccgaa tggaagaaca gcgtagcccc ggtagtcggg tacagggcag cggcaatatt
     1141 cgtaccctga tgacaggttt taccttcacc ctggaaaact atccgacagc cgaagtgaac
     1201 cgggaatacc tgctggtaca gaccacgcta tttatacagg acaacgcgca gcacagcggc
     1261 caggatcagc attttactta ctcaaccagc tttgaactgc atcccaccgg cgaagtttac
     1321 cgtccacaac gaaccctgag caaaccccac actaaaggcc cgcaaagtgc catcgtcacc
     1381 ggcccggcag ggcaggaaat ctggacagat aaatatggcc gggtgaaagt tcagtttggc
     1441 cgggatcgtt acggtaagaa tgacgaaaac agttcctgct gggtccgtgt cagttatccg
     1501 tgggcgggta aaggattcgg tggcattcag atcccgcgca ttggtcagga agtactggtg
     1561 gatttcaaaa acggtgatcc ggatttaccg ataatcgttg ggcgtaccta caaccaggac
     1621 accatgccac cgtggggact accgggcgca gcgacgcaaa gcgggtttta cagccacact
     1681 atcggcggcg gcccaaccaa tgccaacgca ctacgttttg aggataaacc tggcggtgaa
     1741 gaggtctggc tacatgccga aaaagatcag cggattgaag ttaataataa tgaaagccac
     1801 tgggtgggca ataatcgcct taaagtcatt gataaaacag aaacagcgat aattggtgag
     1861 aaacgttctc ttacagttca gatggatgat acctcccttg ccggacaaaa taaaacgatt
     1921 caggccgtac agaatttacg ccttgctgcg ggagattcca taattcttag ttgt
//
LOCUS       DADSYM010000262         1968 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2415
            SAMN08039579-rid11723173.denovo.264, whole genome shotgun sequence.
ACCESSION   DADSYM010000262 DADSYM010000000
VERSION     DADSYM010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039579
            Sequence Read Archive: SRR6362533
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1968)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1968)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980162.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 03:39:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,484
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 4,347
            CDSs (with protein)               :: 4,347
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 43 of 85
            Pseudo Genes (incomplete)         :: 37 of 85
            Pseudo Genes (internal stop)      :: 19 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1968
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039579-rid11723173.denovo.264"
                     /strain="LGE2415"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8302 N 95.9628 W"
                     /collection_date="2015-08-24"
     gene            complement(91..1119)
                     /gene="epmB"
                     /locus_tag="J1Y83_004284"
     CDS             complement(91..1119)
                     /gene="epmB"
                     /locus_tag="J1Y83_004284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EF-P beta-lysylation protein EpmB"
                     /protein_id="HBA7632360.1"
                     /translation="MAHIVTLNTPSREDWLTQLADVVTDPDELLRLLNIDADEKLLAG
                     RSAKKLFALRVPRSFIDRMEKGNPDDPLLRQVLTSQDEFVVAPGFSTDPLEEQHSVVP
                     GLLHKYHNRALLLVKGGCAVNCRYCFRRHFPYAENQGNKRNWQTALEYVAAHPELDEM
                     IFSGGDPLMAKDHELDWLLTQLEAIPHIKRLRIHSRLPIVIPARITDALVERFSHSTL
                     QILLVNHINHANEVDETFRQAMAKLRRVGVTLLNQSVLLRGVNDNAQTLANLSNALFD
                     AGVMPYYLHVLDKVQGAAHFMVSDDEARQIMRELLTLVSGYLVPKLAREIGGEPSKTP
                     LDLQLRQQ"
     gene            1161..1727
                     /gene="efp"
                     /locus_tag="J1Y83_004285"
     CDS             1161..1727
                     /gene="efp"
                     /locus_tag="J1Y83_004285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004871142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HBA7632361.1"
                     /translation="MATYYSNDFRAGLKIMLDGEPYAVEASEFVKPGKGQAFARVKLR
                     RLLTGTRVEKTFKSTDSAEGADVVDMNLTYLYNDGEFWHFMNNETFEQLSADAKAIGD
                     NAKWLLDQAECIVTLWNGQPISVTPPNFVELEIVDTDPGLKGDTAGTGGKPATLSTGA
                     VVKVPLFVQIGEVIKVDTRSGEYVSRVK"
     gene            1779..1904
                     /gene="ecnA"
                     /locus_tag="J1Y83_004286"
     CDS             1779..1904
                     /gene="ecnA"
                     /locus_tag="J1Y83_004286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein antitoxin entericidin A"
                     /protein_id="HBA7632362.1"
                     /translation="MMKRLIVLVLLASALLTGCNTARGFGEDIKHLGNSISRTAS"
BASE COUNT          478 a          500 c          493 g          497 t
ORIGIN      
        1 tgttggttat ataaagagta acaacagcaa tgtatatgaa taattaaagg ttatgcaacg
       61 ggcaaagatt aaacttccct gtttaatcag ttactgctgg cgtagctgga gatccagcgg
      121 cgttttgctg ggttcgccgc caatttctcg cgccagttta ggcaccagat atcccgacac
      181 cagtgtcagc aactcacgca taatctgccg tgcttcgtca tcactcacca taaaatgcgc
      241 cgcgccctgt actttatcga gcacatgcag gtaatacggc atcacgcccg catcgaacaa
      301 cgcattactc aggtttgcca gcgtttgcgc gttatcgttc acaccacgta acagaacgct
      361 ctggttcagc aaagtaacgc ccacccggcg caacttagcc atcgcctgac ggaatgtttc
      421 atctacctca ttggcatggt tgatgtgatt caccagcaag atttgcagtg tagaatgaga
      481 aaagcgttca accagcgcat ctgtgatgcg tgccgggatc acaatcggca gacggctgtg
      541 aatccgcaga cgtttgatat gcgggatggc ttccagttgt gtgagcaacc agtccagctc
      601 gtgatctttc gccatcagcg gatcgccgcc ggagaaaatc atctcgtcca gttccggatg
      661 cgcagcaaca tactcaagtg cagtctgcca gttacgcttg ttgccctgat tttcggcata
      721 ggggaagtgg cgacggaagc aatagcggca atttaccgcg cagccgcctt tgaccagcaa
      781 aagcgcccgg ttgtggtatt tatgcaacaa accaggcact acgctatgct gttcttccag
      841 cgggtcggtg gagaatccgg gcgcgacgac aaactcatct tgcgaggtaa gtacctgacg
      901 caaaagagga tcgtccggat tgcctttctc catgcgatcg ataaatgagc ggggcacacg
      961 cagggcaaac agctttttgg cgctgcgtcc ggccaacagt ttttcgtccg cgtctatatt
     1021 caaaagacgc agaagttcat caggatcggt cacaacatcg gcaagttgcg ttaaccaatc
     1081 ttctctggat ggggtattta gggttacaat atgcgccatt ttgtggctta gctaccaatt
     1141 aacaaatttc agagggcctt atggcaacgt actatagcaa cgattttcgt gctggtctta
     1201 aaatcatgtt agacggcgaa ccttacgcgg ttgaagcgag tgaattcgta aaaccgggta
     1261 aaggccaggc atttgctcgc gttaaactgc gtcgtctgct gaccggtact cgcgtagaaa
     1321 aaaccttcaa atctactgat tccgctgaag gcgctgatgt tgtcgatatg aacctgactt
     1381 acctgtacaa cgacggtgag ttctggcact tcatgaacaa cgaaactttc gagcagctgt
     1441 ctgctgatgc aaaagcaatt ggcgacaacg ctaaatggtt gctggatcag gcagagtgta
     1501 tcgtaactct gtggaatggt cagccgatct ccgttactcc gccgaacttc gttgaactgg
     1561 aaatcgttga taccgatccg ggcctgaaag gtgacaccgc aggtactggc ggcaaaccgg
     1621 ctaccctgtc tactggcgct gtggttaaag ttccgctgtt cgtacagatc ggcgaagtca
     1681 tcaaagtgga tacccgctct ggtgaatacg tctctcgcgt gaagtaatgc ggttgtggtg
     1741 cggcctgcag gctgcaccat cactttttca ggtcagagat gatgaaacgc cttatcgttc
     1801 ttgttttgct tgccagcgcg ctgctcacgg gctgtaacac cgctcgcggt ttcggcgaag
     1861 atatcaaaca tctcggcaac tccatctccc gcactgccag ctaatttttc ttctcttccg
     1921 aaaaatcatc agatttccgt catttttggc gatgttgtct attattaa
//
LOCUS       DADSYM010000263         1927 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2415
            SAMN08039579-rid11723173.denovo.265, whole genome shotgun sequence.
ACCESSION   DADSYM010000263 DADSYM010000000
VERSION     DADSYM010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039579
            Sequence Read Archive: SRR6362533
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1927)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1927)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980162.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 03:39:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,484
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 4,347
            CDSs (with protein)               :: 4,347
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 43 of 85
            Pseudo Genes (incomplete)         :: 37 of 85
            Pseudo Genes (internal stop)      :: 19 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1927
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039579-rid11723173.denovo.265"
                     /strain="LGE2415"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8302 N 95.9628 W"
                     /collection_date="2015-08-24"
     gene            1..732
                     /locus_tag="J1Y83_004287"
                     /pseudo
     CDS             1..732
                     /locus_tag="J1Y83_004287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310454.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="ShET2/EspL2 family type III secretion system
                     effector toxin"
     gene            903..1010
                     /locus_tag="J1Y83_004288"
     CDS             903..1010
                     /locus_tag="J1Y83_004288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588455.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA7632363.1"
                     /translation="MPTKRFDKKHWKMVVVLLAICGAMLLLRWAAMIWG"
     gene            complement(1063..1821)
                     /locus_tag="J1Y83_004289"
     CDS             complement(1063..1821)
                     /locus_tag="J1Y83_004289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiY family protein"
                     /protein_id="HBA7632364.1"
                     /translation="MKLLKTVPAIVMLAGGMFASLNAAADDSVFTVMDDPASAKKPFE
                     GNLNAGYLAQSGNTKSSSLTADTTMTWYGQTTAWSLWGNASNTSSNDERSSEKYAAGG
                     RSRFNLTDYDYLFGQASWLTDRYNGYRERDVLTAGYGRQFLNGPVHSFRFEFGPGVRY
                     DKYTDNASETQPLGYASGAYAWQLTDNAKFTQGVSVFGAEDTTLNSESALNVAINEHF
                     GLKVAYNVTWNSEPPESAPEHTDRRTTLSLGYSM"
BASE COUNT          578 a          438 c          391 g          520 t
ORIGIN      
        1 aaatcatttc cttattcctg gaaaccacgc ctattaaagc taaccatttg cgaagcatac
       61 tggattatat tgaacaggaa cagttaactg ccgaaaaacg taaccattgc atgaaactgt
      121 ccaaaaaaat tcatagagaa aaaaccatac agccaacagt aaatctcaac gatagtgcat
      181 ttttttgcaa tctccttctg acactatttt ttgtcgccat ctgtcattaa aatacgccct
      241 tgattcattg agaaatggaa aaggcaaagt caacctgatt aaacattact cctccgttga
      301 atccacacag cagcatgttc ccttagtccg ggacgcggaa ttcagagcat tacttcgcca
      361 tcctcttgca aggagtcgcg ttatcgcgag tcaggatttt ggctttgctt tagatatttt
      421 cttctgtcga atgatggcaa acaatgtcag tcatatgtcc gcgattttat atatagacaa
      481 tcatactttg tcagtaagta aagatgtcgt gttatcttat atatcaacgt tgggtacttt
      541 tgaaaaaaat attcttttag ccaacgtcag ttatttacac tgttggccgc taaaaatcat
      601 gacaacatgt cagctgttca tatagccatt catcataatc atcataaaac tgtagaaaca
      661 tattatgctg ctataaatgt aatcagccaa agcctgagtt ttagtgctga tgaactaaag
      721 acgtatttat aacccgctat ttatgatatt tgtatatcga tacataattt ttttcataaa
      781 atgcagaaac agtgaccagg tgtacaacgc cccccgacga ctgtaataac aaaaaatccc
      841 ttgatgcctg tcccttttgt tacactccgt tatcacgcac aagagatatg caggacactg
      901 gtatgccgac taaacgcttt gataaaaaac actggaagat ggtggtggtg ctactggcaa
      961 tctgtggcgc tatgttgttg ctacgttggg cagcaatgat ttggggctga gcagtgacaa
     1021 acgaaaacag ccagaccgca taacacggtc cggcagataa ttttacatgc tgtaacccaa
     1081 cgataaggtt gtccgacgat cggtatgttc cggtgcagac tctggaggtt cagagttcca
     1141 tgtaacgttg taagccacct tcagtccaaa atgttcatta atagcaacat ttaatgcact
     1201 ttcggagttc agcgttgtgt cttccgcgcc aaagaccgaa acaccctgcg taaatttagc
     1261 gttgtcagtc aactgccatg cataagcccc ggaggcatag cccagcggct gggtttcact
     1321 ggcattgtcg gtatatttgt cgtaacgcac accagggccg aattcaaagc ggaaactgtg
     1381 taccgggcca ttcaaaaact ggcgaccata acccgcggtc aacacatcgc gctctcgata
     1441 gccgttataa cggtctgtca gccagcttgc ctgaccaaat aaatagtcat aatcagttaa
     1501 attaaaacgg ctacgcccgc ccgccgcata tttttctgaa gaacgctcat cattagaaga
     1561 agtattactg gcgttccccc acagtgacca ggcagtggtt tgtccatacc aggtcatagt
     1621 agtgtcagcc gtaagtgagg agcttttcgt attgcctgat tgtgcaagat atcctgcgtt
     1681 cagattacct tcgaaaggtt ttttagcgct ggcaggatcg tccatgacag taaaaacgga
     1741 atcatcagca gctgcattca gtgacgcaaa catgcccccc gccaacataa cgatggcagg
     1801 aactgtcttc aaaagcttca tttatcaaga gtccgtacaa caaaaaaaga gaccatcgcg
     1861 gtcccggaaa ctttcttaag gatcaaagat tagcgtccct ggaaaggtaa cgaattataa
     1921 aaaggcg
//
LOCUS       DADSYM010000264         1908 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2415
            SAMN08039579-rid11723173.denovo.266, whole genome shotgun sequence.
ACCESSION   DADSYM010000264 DADSYM010000000
VERSION     DADSYM010000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039579
            Sequence Read Archive: SRR6362533
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1908)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1908)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980162.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 03:39:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,484
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 4,347
            CDSs (with protein)               :: 4,347
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 43 of 85
            Pseudo Genes (incomplete)         :: 37 of 85
            Pseudo Genes (internal stop)      :: 19 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1908
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039579-rid11723173.denovo.266"
                     /strain="LGE2415"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8302 N 95.9628 W"
                     /collection_date="2015-08-24"
     gene            complement(<1..702)
                     /locus_tag="J1Y83_004290"
     CDS             complement(<1..702)
                     /locus_tag="J1Y83_004290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector protein NleH"
                     /protein_id="HBA7632365.1"
                     /translation="MLSPSSVNLGCSWNSLTRNLTSPDSRILSSVRDAAASSDNGAQV
                     KVGNRTYRVVVTDNKFCVTRESHSGCFTNLLHRLGWPKGEISRKIEVMLNSSPVNRTM
                     ERGAVHSNRPDLPPVDYAPPELPSVDYNRLPVPGNVIGKGGNAVVYEDAEDATKVLKM
                     FTASQSNEEVTNEVRCFNQYYGAGSAEKIYGDNGDIIGIRMDKINGESLLNISSLPAQ
                     AEHAIYDMFDRLEQKG"
     gene            complement(866..997)
                     /locus_tag="J1Y83_004291"
     CDS             complement(866..997)
                     /locus_tag="J1Y83_004291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001407642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HBA7632366.1"
                     /translation="MAFIFRMLQIATDEETSLLTVWKRYRVLLNRVDTSVSPDFEGQ"
     gene            complement(1023..1310)
                     /gene="nleC"
                     /locus_tag="J1Y83_004292"
                     /pseudo
     CDS             complement(1023..1310)
                     /gene="nleC"
                     /locus_tag="J1Y83_004292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308874.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector zinc
                     metalloprotease NleC"
     gene            complement(1344..>1908)
                     /gene="nleB2"
                     /locus_tag="J1Y83_004293"
     CDS             complement(1344..>1908)
                     /gene="nleB2"
                     /locus_tag="J1Y83_004293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type III secretion system effector arginine
                     glycosyltransferase NleB2"
                     /protein_id="HBA7632367.1"
                     /translation="RLRTEDKRDEYLLNLLEKELREISKEQDSLISMYAKKRNHAWFD
                     FFRNLALLKAGEIFRCTYNTKNHGISFGEGCIYLDMDMILTGKLGTIYAPDGISMHVD
                     RRNDSVNIENSAIIVNRSNHPALLEGLSFMHSKVDAHPYYDGLGKGVKKYFNFTPLHN
                     YNHFCDFIEFKHPNMIMNTSQYTCSSW"
BASE COUNT          584 a          440 c          304 g          580 t
ORIGIN      
        1 tcctttttgc tcaagcctgt caaacatatc ataaatggca tgctcagcct gtgccggcaa
       61 ggacgaaata tttaaaagcg attctccatt tattttatcc attctaatac caataatatc
      121 gccattatcg ccatatattt tttctgcact cccggcacca taatattggt tgaagcaacg
      181 aacttcgttt gttacctctt cattgctttg agatgcagta aacatcttca ggacttttgt
      241 tgcatcctca gcatcttcat atactacagc gttaccccct ttgccaataa cattaccagg
      301 cacaggcaac ctgttatagt ccacactcgg taactctggc ggtgcataat caacaggagg
      361 taaatcaggt ctattcgaat gaacagcacc tctttccata gtcctattca ctggcgatga
      421 attcagcatc acctcaattt tcctgctaat ctctccctta ggccatccca gcctgtgcaa
      481 cagattagta aagcaaccac tatgactttc tcttgtaacg caaaacttat tatcagtgac
      541 aacaacacga tatgttctgt tgcccacctt tacttgcgcc ccattatccg agctagcagc
      601 tgcatccctt acagaggata aaatacgact gtcaggagaa gtcaggtttc tggttaaaga
      661 attccatgaa caccccaaat ttacagaaga tggtgatagc atacatttca accttcaaaa
      721 tgaatcaatc tttactttct taacaaacat caccatgaca tgacaacaaa aaactggaaa
      781 ttaactattg tttttgagta tctggcaact cattcatcct tccagcatcc cgtcagacga
      841 acgtttacga actataaatc aggtattact ggccctcgaa atctggagat actgacgtat
      901 caacacggtt cagtaacacc cgatacctct tccagactgt caacaacgat gtctcttcat
      961 ccgttgcgat ctgcagcatc ctgaatatga atgccatagc ttacggcatc acgaaaagtt
     1021 tatgagcggc tcaaagcatc aagtatagta ttattaatca tatcgaccgt atgctgatca
     1081 atgagtctgc cacgactgtc atggacggca cgaattacac tttcgtatac gttgcttaaa
     1141 tctgctgcgg aaagtggtat tcgcttgcct atatccaaaa cgtaatccgc ataggcattt
     1201 tcagcacgat taggagcaat gggagcagag tatcctgccg gggcggaaaa gttgaagttg
     1261 gactgtaatg agggaatttt catgttgata cctaatacca tgcaaatcat ttattacaac
     1321 agaataaact ttatcaaatt catttaccat gaactgcatg tatactgact tgtgttcatg
     1381 atcatattag ggtgtttaaa ctcaataaag tcacaaaaat gattataatt atgtaatggt
     1441 gtaaagttaa aatatttctt aactcctttc cccaaaccat cataatatgg atgagcatct
     1501 actttactat gcataaaaga aagtccctca agtagagcag gatgattact acggttaaca
     1561 attattgcac tattttcaat atttacacta tcattacgac gatccacatg cattgaaatt
     1621 ccatcaggag catatattgt accaagctta cctgtaagta tcatatccat atcaagatag
     1681 atacacccct ccccgaatga aataccgtga ttctttgtat tatatgtgca cctgaatatc
     1741 tctcctgctt ttaataaggc taaatttctg aagaaatcaa accatgcatg atttcttttc
     1801 tttgcataca tagaaatcaa agaatcctgc tcctttgaaa tttccctcag ctctttctct
     1861 aacagattca acaagtattc atctcttttg tcttcagttc gtaacctt
//
LOCUS       DADSYM010000265         1894 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2415
            SAMN08039579-rid11723173.denovo.267, whole genome shotgun sequence.
ACCESSION   DADSYM010000265 DADSYM010000000
VERSION     DADSYM010000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039579
            Sequence Read Archive: SRR6362533
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1894)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1894)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980162.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 03:39:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,484
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 4,347
            CDSs (with protein)               :: 4,347
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 43 of 85
            Pseudo Genes (incomplete)         :: 37 of 85
            Pseudo Genes (internal stop)      :: 19 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1894
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039579-rid11723173.denovo.267"
                     /strain="LGE2415"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8302 N 95.9628 W"
                     /collection_date="2015-08-24"
     gene            <1..1485
                     /locus_tag="J1Y83_004294"
     CDS             <1..1485
                     /locus_tag="J1Y83_004294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000514812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1983 domain-containing protein"
                     /protein_id="HBA7632368.1"
                     /translation="PTVQFEFWFSETKISDVSQVDKSARYLGTALYWVASGQNIKPGH
                     DYYFYVRSVNTVGKSAFVEAVGQPGNNPEEYLNFFEGKINSTLLGQELNDRINASALR
                     SEVEQLEDEVNQRLESDIAGVTQKIGETENSLTQLVAKKNDEQSLAISQVSQKVDNVS
                     SELTQTVSQSNEENARQIAQVRQYVDEKSSEIISTTDKKLGEQEATIQQIQKVQTDTS
                     NNLNSMWAVKLQQMQDGRLYIAGIGAGIENTPDGMQSQVLLAADRIAFINPENGNTTP
                     ALVTQGGQTFINEALIKTLIAPTITSGGNPPAFSLTSDGKLTAKNADISGSVNANSGT
                     LNNVTINENCQIKGKLSANQIEGDIVKTVSKSFPRTSTYASGTITVTISDDQKFDRQV
                     MIPALLFKGSRKENYGSNNQQSYVYSVCRLQVTKNGTEIFNQSTTDAPAVFSSVIDMP
                     AGQGTLTLKFTVSSSMVNNWTPTTSISDLLVVVMKKSTAGITIS"
     gene            1553..>1894
                     /locus_tag="J1Y83_004295"
     CDS             1553..>1894
                     /locus_tag="J1Y83_004295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ail/Lom family outer membrane beta-barrel
                     protein"
                     /protein_id="HBA7632369.1"
                     /translation="MRKVCAAMLSAAICLAVSGAPAWASEQQATLSAGYLHARTNAPG
                     SDNLNGINVKYRYEFTDTLGLVTSFSYAGDRNRQLTRYSDTRWHEDSVRNRWFSVMAG
                     PSVRVNEWFSAY"
BASE COUNT          519 a          424 c          511 g          440 t
ORIGIN      
        1 ccgacggtac agtttgagtt ctggttttct gaaacgaaga tttcagatgt ctcacaggtg
       61 gacaaatctg cccgctatct tggtacggca ctgtactggg tagcctccgg ccagaatatt
      121 aagccgggcc atgattatta cttttatgtt cgcagtgtga ataccgttgg taaatcggca
      181 ttcgtggagg ctgtcggcca gccgggtaat aatcctgaag agtatctcaa tttttttgaa
      241 gggaaaataa acagcaccct gctggggcag gagctgaatg atcgtattaa tgcctctgca
      301 ttgcgcagtg aagttgagca actggaggat gaggtcaatc agcgactgga gagtgatatt
      361 gccggggtga cccaaaaaat cggggaaact gaaaacagcc tcacgcagct ggttgctaaa
      421 aaaaatgatg agcagtcact ggccatatcc caggtgagtc agaaagtgga caacgtcagt
      481 agcgaactca cacaaacggt cagtcagagt aatgaggaga atgcacgcca gattgcgcag
      541 gttcgtcagt atgtggatga aaaaagcagt gaaatcatct cgacgacaga caaaaaactg
      601 ggcgagcagg aagcaacaat ccagcagata caaaaggttc agacagacac cagtaataac
      661 ctgaacagta tgtgggccgt gaaactgcag cagatgcagg atggtcgcct ttacattgcg
      721 ggtatcggtg ccggtattga gaatacccct gacggtatgc agagtcaggt gctgctggcg
      781 gcagacagga ttgcgttcat taatccggag aacggtaaca ccactccagc actggtcacg
      841 caggggggac agacgtttat caatgaagcg ctgattaaga cccttattgc tcccacaatt
      901 accagtggtg gcaatcctcc tgcattttcc ctgacatcag acggaaagct gaccgctaaa
      961 aatgcggata tcagtggcag tgtgaatgcg aactcaggga cgctcaacaa cgtcacgata
     1021 aatgagaact gtcagattaa ggggaaactg tccgccaacc agattgaagg cgatattgtc
     1081 aaaacggtca gcaagtcttt cccccgcacg agcacttatg ccagcggcac cattacggtc
     1141 acgattagtg atgatcagaa gtttgaccga caggtcatga tcccggctct gctgtttaaa
     1201 ggaagcagga aggaaaatta tggcagtaat aatcaacagt cttatgttta ttctgtatgc
     1261 cgtttgcagg taacgaaaaa cgggacagaa atttttaacc agtctacaac ggatgctccg
     1321 gcggtttttt cttccgttat tgatatgcct gcggggcagg gaacgttgac gttaaagttt
     1381 acggtctctt cttcaatggt caataactgg acaccgacaa ccagtatcag cgatttgctt
     1441 gttgtggtga tgaagaaatc caccgcaggt atcacgatta gctgaatttt ataacccata
     1501 tacgggcgcc agaaatggcg ccttttttat tgcagaaaag cgagaggtaa ttatgcgtaa
     1561 agtttgtgca gccatgttgt ccgcagccat ctgtctggcc gtatccggtg cgcctgcatg
     1621 ggcgtctgag cagcaggcca cgctgagcgc ggggtatctt catgcccgga cgaacgctcc
     1681 cggcagcgat aatcttaacg ggattaacgt gaaataccgt tatgaattta cggacacgct
     1741 ggggctggtg acgtcattca gctatgcagg agacaggaat cgccagctta cccgttacag
     1801 cgatacccgc tggcatgaag attctgtgcg taaccgctgg ttcagcgtca tggcgggacc
     1861 ctctgtgcgc gtgaatgaat ggttcagcgc gtat
//
LOCUS       DADSYM010000266         1839 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2415
            SAMN08039579-rid11723173.denovo.268, whole genome shotgun sequence.
ACCESSION   DADSYM010000266 DADSYM010000000
VERSION     DADSYM010000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039579
            Sequence Read Archive: SRR6362533
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1839)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1839)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980162.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 03:39:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,484
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 4,347
            CDSs (with protein)               :: 4,347
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 43 of 85
            Pseudo Genes (incomplete)         :: 37 of 85
            Pseudo Genes (internal stop)      :: 19 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1839
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039579-rid11723173.denovo.268"
                     /strain="LGE2415"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8302 N 95.9628 W"
                     /collection_date="2015-08-24"
     gene            complement(1..1782)
                     /gene="hcp"
                     /locus_tag="J1Y83_004296"
     CDS             complement(1..1782)
                     /gene="hcp"
                     /locus_tag="J1Y83_004296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020238820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tube protein Hcp"
                     /protein_id="HBA7632370.1"
                     /translation="MGDIVYLRIIGEKQGDISSGCGTYASVGNRWQVGHEDEIFAFAL
                     TNAITSTGKGVNLQGLQFCKLIDKSSPLLSNAINQNERLFIEIDLYRINKSGRWERYY
                     YIQLRNASLTAIHVNISDNNLPTECVNVNYDYILCKHLIANTEFDWLAFPAGYNSLFI
                     PPKNPPASNLNPEPLPVVNLPLSPPAVKPVYAKSCLKEKGCTDAGTAEEPAENFGQVA
                     IFALPVVDDCCGYHHPEANDVGQPAEAQTMLLFPGSVLAAQIWGKWSLSGILSATRGS
                     YIGALASALYIPSAGEGSARVPGRDEFWYEEELRQKALAGSTATTRVRFFWGTDIHGK
                     PQVYGVHTGEGTPYENVRVANMQWNEQTQRYEFTPAHDVDGPLITWTPENPEHGNVPG
                     HTGNDRPPLDQPTILVTPIPDGTDTYTTPPFPVPDPKEFNDYILVFPAGSGIKPIYVY
                     LKEDPRKLPGVVTGRGVPLSPGTRWLDMSVSNNGNGAPIPAHIADKLRGREFKTFDEF
                     REALWLEVSKDSVLLAQFIKSNQNNVSQGYSPYVPEEGYYYGPNEIVKKFQIHHVVAI
                     EHGGGVYDIDNFRIVTPRLHDEIHYRR"
BASE COUNT          473 a          465 c          411 g          490 t
ORIGIN      
        1 ttatctcctg tagtgaattt catcatgtag tcggggcgta acaatcctaa aattatcaat
       61 atcatacact ccaccaccat gttctatagc aactacatga tgtatctgga atttcttaac
      121 tatctcattt ggtccgtaat aatacccctc ctcagggaca tagggggaat atccttgact
      181 aacgttattc tgattacttt ttataaactg cgccaataac actgagtctt tactcacctc
      241 cagccacagc gcctcgcgaa actcatcaaa ggttttaaac tcccgtccgc gcaatttatc
      301 agcaatatgc gccgggattg gtgcgccgtt gccgttattg gataccgaca tatccagcca
      361 gcgagttcct ggtgaaagcg ggacgccgcg ccctgttaca acaccaggca gctttcgcgg
      421 atcctccttc aggtaaacat agatgggctt aataccggat cccgccggaa aaaccagaat
      481 ataatcgttg aattctttcg gatcaggaac cgggaatggc ggcgtggtat aggtatcggt
      541 gccgtccgga atcggcgtca ccagaatggt gggctgatcc agcggcggcc tgtcgttacc
      601 ggtatggccc ggaacattcc catgttccgg attttccggc gtccaggtaa tcagggggcc
      661 atcgacatcg tgagcggggg taaattcata acgctgcgtc tgctcgttcc actgcatgtt
      721 cgccacgcgg acgttttcat acggcgtacc ttcacccgta tgaacaccat acacctgagg
      781 cttgccgtga atgtcagttc cccagaaaaa acgtacccgg gtggtggcgg tactgcctgc
      841 tagtgctttc tgccgcagtt cttcctcata ccagaactca tcacgtccgg gcacgcgagc
      901 actgccctcg cccgcagagg gaatatacaa agcagatgcc aacgcaccga tgtaagagcc
      961 gcgggttgca ctgagtatgc cactgagcga ccattttccc catatttgag ccgccaacac
     1021 actacccgga aacagtagca tggtttgagc ttctgcgggt tgcccaacat cgttagcctc
     1081 cggatggtgg tatccacagc agtcatcaac cactggcaga gcaaaaatcg ctacttgccc
     1141 gaagttttca gcgggttctt ctgccgttcc ggcatctgta catcccttct ccttcagaca
     1201 ggatttggca tagaccggtt taaccgctgg tggagagagt ggaaggttaa caactggtag
     1261 cggctcaggg ttaagattac tggcaggtgg gtttttaggt ggaataaata agctattata
     1321 gccagcagga aaggccaacc agtcaaattc cgtattggct attagatgtt tgcataatat
     1381 gtagtcataa ttaacattta cacattcggt aggaagatta ttgtcagaaa tgtttacatg
     1441 aatagcagtt aatgaagcat ttcttagctg aatataataa taccgttccc agcgcccgct
     1501 tttatttata cgatacaaat cgatttcaat aaataaccgc tcattctgat tgatggcatt
     1561 agacagtagc ggtgagcttt tatcaatgag tttgcaaaat tgtagcccct gtagattaac
     1621 gcctttacca gtactggtaa tggcgttggt gagtgcaaag gcaaaaattt catcttcatg
     1681 gccaacctgc cagcgattac caaccgacgc atacgtccca cagcctgaag aaatatcacc
     1741 ctgcttctca ccgattattc tcaggtaaac aatatcgccc actttatttc attcccgttt
     1801 atccttaact tttttgcatt aaatagaaaa cttatttct
//
LOCUS       DADSYM010000267         1836 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2415
            SAMN08039579-rid11723173.denovo.269, whole genome shotgun sequence.
ACCESSION   DADSYM010000267 DADSYM010000000
VERSION     DADSYM010000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039579
            Sequence Read Archive: SRR6362533
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1836)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1836)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980162.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 03:39:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,484
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 4,347
            CDSs (with protein)               :: 4,347
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 43 of 85
            Pseudo Genes (incomplete)         :: 37 of 85
            Pseudo Genes (internal stop)      :: 19 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1836
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039579-rid11723173.denovo.269"
                     /strain="LGE2415"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8302 N 95.9628 W"
                     /collection_date="2015-08-24"
     gene            complement(26..349)
                     /locus_tag="J1Y83_004297"
     CDS             complement(26..349)
                     /locus_tag="J1Y83_004297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA7632371.1"
                     /translation="MRLLILTLSLITLTGCTVTRQAHVSEVDAATGIVRLVYDQAFLQ
                     HARTDRYVSRGIADRACQQAGYTHAIPFGQPVGNCSLFAGSLCLNTEFTLSYQCHHSA
                     FPVFL"
     gene            613..699
                     /gene="azuC"
                     /locus_tag="J1Y83_004298"
     CDS             613..699
                     /gene="azuC"
                     /locus_tag="J1Y83_004298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein AzuC"
                     /protein_id="HBA7632372.1"
                     /translation="MKLRKILKSMFNNYCKTFKDVPPGNMFR"
     gene            813..1064
                     /locus_tag="J1Y83_004299"
     CDS             813..1064
                     /locus_tag="J1Y83_004299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416416.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2766 domain-containing protein"
                     /protein_id="HBA7632373.1"
                     /translation="MSQPLNADQELVSDVVACQLVIKQILDVLDVIAPVEVREKMSSQ
                     LKNIDFTNHPAAADPVTMRAIQKAIALIELKFTPQGESH"
     gene            complement(1143..1646)
                     /locus_tag="J1Y83_004300"
     CDS             complement(1143..1646)
                     /locus_tag="J1Y83_004300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme ferritin-like protein"
                     /protein_id="HBA7632374.1"
                     /translation="MATAGMLLKLNSQMNREFYASNLYLHLSNWCSEQSLNGTATFLR
                     AQAQSNVTQMMRMFNFMKSVGATPIVKAIDVPGEKLNSLEELFQKTMEEYEQRSSTLA
                     QLADEAKELNDDSTVNFLRDLEKEQQHDGLLLQTILDEVRSAKLAGMCPVQTDQHVLN
                     VVSHQLH"
BASE COUNT          524 a          402 c          435 g          475 t
ORIGIN      
        1 agatccccct cgctgattaa aacgtttaga gaaagacagg gaaggcggaa tgatggcact
       61 gatatgacaa agtgaattcc gtattcaaac ataaggaacc cgcaaagagg ctacagttgc
      121 ctactggctg accaaagggg atagcgtggg tatagcctgc ctgctggcat gcgcgatcgg
      181 cgatcccgcg actgacataa cgatcggtac gggcatgctg caaaaaagcc tgatcataaa
      241 ccaaccgaac aatacctgtt gccgcgtcaa cttcgctcac atgggcctgg cgagtaaccg
      301 tacatcctgt cagcgttatt aaggaaagag tcagaatcag aagtctcatg gtccagccag
      361 aagagtaaaa gaaagcagta ttttacgcgt tacagcgctg cgacaatggc cggaaaagac
      421 gcctggcagg cgctatttct tgccatctgt atgtgtcaga aattgggggg gctgcttttt
      481 gattatcatt tttataacat taaaggggta tttatgtgat ttacatcaca taaagattga
      541 tttttgtgat ggttgtcata ttataaacgt aaagacaata acacctgtat aacaaatggt
      601 cggagtaccg cgatgaaact gcgcaagatc ctgaaaagta tgttcaataa ctattgcaag
      661 acgttcaaag acgtaccgcc aggcaatatg ttccgataac aaaaaacctg ctccggcagg
      721 ttttttgtgt tctgatgacg gtggatgtga ctatcgctga agatgtgaag ccgctactat
      781 aacggctttc aaaatgctat caggagttta cgatgtccca gccgctgaat gccgatcagg
      841 aactggtttc tgatgtggtt gcttgccagt tggttatcaa acaaattctg gatgttctcg
      901 atgtgatcgc gcccgttgaa gttcgcgaaa aaatgtccag ccagctgaag aatatcgact
      961 ttactaacca tcccgccgcc gcagacccgg tcaccatgcg ggcaatccaa aaagccatcg
     1021 cgttgattga actcaagttt accccacagg gcgaatccca ttaaaaaacc gctgcttcaa
     1081 aaaaatgagg cagcggtttc tcaaaaactt catcggcgca atgcattagc gccgatgtat
     1141 gatcaatgca gctgatgcga cacgacattc agaacatgtt ggtcggtctg cacaggacac
     1201 ataccagcaa gtttcgcact gcgcacttca tcaagaatgg tttgcagtaa caggccatca
     1261 tgctgctgtt ctttttccag atcgcgcagg aaattgacgg ttgaatcatc attcagttct
     1321 ttcgcttcat cggctaactg cgccagcgtg cttgaacgtt gctcgtattc ttccatcgtt
     1381 ttttggaaca gttcttccag agagttcagt ttttcaccgg gaacatcaat ggctttaacg
     1441 atgggggtag cgccgacact cttcataaag ttaaacatgc gcatcatttg ggtcacatta
     1501 ctctgtgcct gggcgcgaag gaaagtggcg gtgccgttca gactctgttc agaacaccag
     1561 ttactcaggt gaaggtagag attggatgcg taaaactcgc ggttcatttg agagttgagt
     1621 ttgagaagca ttccagcggt tgccatacct aatatcctta tatccagaag tggagaggtg
     1681 caagataaat caaaacacca taattactgt ttgcagaata attcctttct gccggtccga
     1741 aatgtgatcg tggagtcaat tctgacgatg atctgaaata agaataacgt ctgaacgggt
     1801 ataagaaaat gcttaaatca tcaagtttat atattt
//
LOCUS       DADSYM010000268         1817 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2415
            SAMN08039579-rid11723173.denovo.270, whole genome shotgun sequence.
ACCESSION   DADSYM010000268 DADSYM010000000
VERSION     DADSYM010000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039579
            Sequence Read Archive: SRR6362533
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1817)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1817)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980162.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 03:39:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,484
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 4,347
            CDSs (with protein)               :: 4,347
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 43 of 85
            Pseudo Genes (incomplete)         :: 37 of 85
            Pseudo Genes (internal stop)      :: 19 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1817
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039579-rid11723173.denovo.270"
                     /strain="LGE2415"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8302 N 95.9628 W"
                     /collection_date="2015-08-24"
     gene            <1..1758
                     /locus_tag="J1Y83_004301"
     CDS             <1..1758
                     /locus_tag="J1Y83_004301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001533319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sel1 repeat family protein"
                     /protein_id="HBA7632375.1"
                     /translation="PINMSRGIVHPRPVMTFRAPNTFSGHPLPDVTIKSEGEFTWRAV
                     LDMLCEENEATPEHAPMQLAKALSFLEKKDLSSRMCFEETLLLHLHRVFRPLLTLAAA
                     QTFRWHLVAGSRQPVIQTEINESCLLYYRIEKEIASCFYSTMSPSEELENGKALLSSY
                     QQLQEDDRSHKWFKVALLNLINKYDLSDDYLSLIIELIGWSFQPDPHQFCWQVSEQFY
                     VLFNLHKTGFEKILFALNLEQGKYQQSKKLTSYKRAAQQGNSVFQNDLGAMYYIGEII
                     KKDFVQAKYWFEKSAGQGNNDALLNLALMYRDGKGVSKNPQKAISLYLNAANKNHPLA
                     QHSLACMYRDGEGVEVDDEQAFKWCQKSAEQGYAEAQYHLATMYIDGRGVDVDYQQVV
                     HWLNLSAEQKNSKAEYTLGYMYYKGTEIPQDMTMAINLLKAAADHGDIDAALVLGQLY
                     VHGREGYLPKDIHQAIAYLNQALEGGEEQSAEILGMIYYQGFEEIAENSALSFNMFFK
                     AAYAGIKRAQRMLSIMYMEGDGTDKNYILSWAWGIISEAYHDLESNDWLPEVRESLSL
                     QDLSRAQRMARILKKRYQL"
BASE COUNT          585 a          320 c          407 g          505 t
ORIGIN      
        1 ccgatcaata tgtccagggg tattgttcat cctcgtcctg tgatgacatt tcgcgcaccc
       61 aacacctttt ctggtcatcc acttcctgat gtcactatta aatcagaggg ggaattcacc
      121 tggcgagccg tgctggatat gctctgtgag gagaatgaag caacccccga acacgccccc
      181 atgcaacttg ctaaggcgtt atctttcctt gagaaaaaag atctcagctc acgaatgtgc
      241 tttgaagaaa cgttattact gcatcttcat cgggtattcc ggccattatt aactctcgct
      301 gcagcacaaa cgttcagatg gcatttagtc gcaggcagtc gtcaacccgt aatccagaca
      361 gaaattaacg aaagttgtct tctttattat cgcatcgaaa aagaaatagc cagttgtttt
      421 tacagtacga tgtcaccttc tgaagagtta gaaaatggca aggcgctgtt gtcttcttat
      481 cagcaactac aagaagatga cagaagtcat aaatggttca aagtggcttt attaaacctc
      541 atcaataaat atgatttatc tgatgattat ttaagtttaa ttattgagtt aatagggtgg
      601 tcatttcaac ctgacccaca ccagttctgc tggcaggtat cagaacagtt ttatgtattg
      661 tttaatctac ataaaacagg atttgaaaaa atacttttcg ccttaaatct tgagcaaggg
      721 aaatatcaac aatcaaaaaa attaacaagt tataaacgtg ccgcgcagca aggaaattcg
      781 gtgtttcaaa atgacttagg tgcaatgtat tatattggcg agattatcaa aaaagacttt
      841 gtgcaggcaa aatattggtt cgagaaatcc gcagggcaag gcaataacga tgcgttatta
      901 aatttggcgt taatgtatcg tgatggtaaa ggtgtaagta aaaatccaca aaaagctatt
      961 tcattatatc taaacgcggc taataaaaat catcctctgg cgcagcatag tttggcatgt
     1021 atgtatcggg atggtgaagg tgtggaagtt gacgatgaac aggcctttaa atggtgtcaa
     1081 aaatcggcag aacaagggta cgctgaagca caatatcacc ttgccacaat gtacatagac
     1141 ggtcggggcg ttgatgtgga ttatcaacaa gttgttcact ggctaaattt atcagcagaa
     1201 cagaaaaata gtaaagcaga gtatacgttg ggatatatgt attataaagg aacagagatc
     1261 cctcaagata tgacaatggc aattaatctg ttaaaagctg ccgctgacca tggagatatt
     1321 gatgcagcct tagttttagg tcaactttat gttcatggca gggaaggata cttgcctaaa
     1381 gatatccacc aggccattgc gtatttaaat caagccctgg aaggaggcga ggaacagtcc
     1441 gccgagatat taggtatgat ctattaccag ggatttgaag aaattgcaga aaactcagct
     1501 ttaagtttta acatgttttt taaagcggcg tacgctggaa ttaaaagagc gcaaagaatg
     1561 ctttctatta tgtatatgga aggggatggt acagataaaa attacatttt gtcatgggcg
     1621 tgggggataa tttctgaagc atatcatgat ttggagagca atgactggtt gccagaagtc
     1681 agagaaagtt tgtcgttgca agatttaagc cgggcgcaac ggatggcgag gattctgaaa
     1741 aaacgttatc aattataaat tactttcacg gaaaagagag gcataatgaa ctgctgggaa
     1801 atattaggtt tacaatc
//
LOCUS       DADSYM010000269         1816 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2415
            SAMN08039579-rid11723173.denovo.271, whole genome shotgun sequence.
ACCESSION   DADSYM010000269 DADSYM010000000
VERSION     DADSYM010000269.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039579
            Sequence Read Archive: SRR6362533
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1816)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1816)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980162.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 03:39:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,484
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 4,347
            CDSs (with protein)               :: 4,347
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 43 of 85
            Pseudo Genes (incomplete)         :: 37 of 85
            Pseudo Genes (internal stop)      :: 19 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1816
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039579-rid11723173.denovo.271"
                     /strain="LGE2415"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8302 N 95.9628 W"
                     /collection_date="2015-08-24"
     gene            complement(243..836)
                     /gene="tehB"
                     /locus_tag="J1Y83_004302"
     CDS             complement(243..836)
                     /gene="tehB"
                     /locus_tag="J1Y83_004302"
                     /EC_number="2.1.1.265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance methyltransferase TehB"
                     /protein_id="HBA7632376.1"
                     /translation="MIIRDENYFTDKYELTRTHSEVLEAVKVVKPGKTLDLGCGNGRN
                     SLYLAANGYDVDAWDKNAMSIANVERIKSIENLDNLHTRVVDLNNLTFDGQYDFILST
                     VVLMFLEAKTIPGLIANMQRCTKPGGYNLIVAAMDTADYPCTVGFPFAFKEGELRRYY
                     EGWEMVKYNEDVGELHRTDANGNRIKLRFATMLARKK"
     gene            complement(833..>1816)
                     /gene="tehA"
                     /locus_tag="J1Y83_004303"
     CDS             complement(833..>1816)
                     /gene="tehA"
                     /locus_tag="J1Y83_004303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate transporter/tellurite-resistance
                     protein TehA"
                     /protein_id="HBA7632377.1"
                     /translation="DKVLNLPAGYFGIVLGTIGMGFAWRYASQVWQVSHWLGDGLVIL
                     AMIIWGLLTGAFITRLIRFPHSVLAEVRHPVLSSFVSLFPATTMLVAIGFVPWFRPLA
                     VCLFSFGVVVQLAYAAWQTAGLWRGSHPEEATTPGLYLPTVANNFISAMACGALGYTD
                     AGLVFLGAGVFSWLSLEPVILQRLRSSGELPTALRTSLGIQLAPALVACSAWLSVNGG
                     EGDTLAKMLFGYGLLQLLFMLRLMPWYLSQPFNASFWSFSFGVSALATTGLHLGSGSD
                     NGFFHTLAVPLFIFTNFIIAILLIRTFALLMQGKLLVRTERAVLMKAEDKE"
BASE COUNT          518 a          482 c          405 g          411 t
ORIGIN      
        1 gttgtggcaa aaaaagtgtt ggtgaaaata aattttttag ccccttttca gacgacatag
       61 tcaatgccga aaaaggggct aagtcaatca tcagtagtta aatttcagta agttaagcgg
      121 ctaaaaaact tcgactactg tgattaattt cacatccctt cgataaaaaa tatgcttacc
      181 gaaaagaaga acagtaaacc atctcagcgt ggggaatttt cacgcgcttt gttattaccg
      241 gatcattttt tacgtgccag catcgtggcg aaacgcagtt taatacgatt accgttggcg
      301 tcggtgcggt gcagttcgcc gacgtcttca ttgtatttca ccatctccca gccttcgtaa
      361 tatcgacgta attccccctc tttgaaggca aacgggaagc cgacggtaca tggataatca
      421 gcggtatcca tcgccgccac aatcaggttg taaccgccag gtttagtgca acgttgcata
      481 ttggcaatca gccctgggat ggttttagcc tcaaggaaca tcagcaccac agtagaaaga
      541 ataaaatcgt actgtccatc aaatgtgagg ttattcagat caacgacccg ggtgtgtaaa
      601 ttatccagat tttcaatgga cttaatgcgc tcgacgttgg cgatactcat ggcattttta
      661 tcccatgcgt caacatcata accattggct gccaggtaga gactgttacg accattgcca
      721 cagcccagat ccagcgtttt acccggttta accactttca ccgcttccag tacttcagag
      781 tgtgtgcgag ttaattcata tttatcagta aaatagtttt catcacgaat gatcattctt
      841 tgtcctctgc tttcattaaa acggcgcgct cggttctgac taacaatttt ccctgcatca
      901 gaagcgcaaa agtacggatg agcagtattg caataataaa attggtaaag ataaacagcg
      961 gcaccgccag cgtatggaaa aatccattat cgctgccact ccccagatgc aaaccggtgg
     1021 ttgccagtgc agatacaccg aacgagaaac tccagaatga agcattaaat ggctgggaga
     1081 gataccatgg catcagacgt agcataaaca gcagttgcag cagtccataa ccgaaaagca
     1141 ttttcgccag cgtgtcaccc tcgccgccgt tgacgctcag ccaggcacta caggccacca
     1201 gcgcaggagc gagctgaatg ccgagtgatg tccgcagtgc cgtgggtaat tctcccgaac
     1261 tgcgcagacg ctgcaagatc accggttcca ggcttagcca tgagaaaacg cctgcgccta
     1321 aaaacaccag accggcgtcg gtgtagccca acgcaccaca ggccattgcg ctgataaagt
     1381 tgttggcaac tgtcggcaga tacagtccag gcgtggtagc ttcttcaggg tgagatccgc
     1441 gccataatcc cgcagtttgc catgcggcat aagccaactg aaccacgaca ccaaaactga
     1501 acaggcacac cgccagtggg cgaaaccacg gaacaaaacc aatcgccacc agcatcgtcg
     1561 ttgccggaaa caaactcaca aaactgctca gcactggatg gcgcacttct gccagcacgc
     1621 tatgcggaaa gcgtatgagt cgggtaataa atgcgccagt caataatccc cagatgatca
     1681 tcgccagaat caccagccca tcccccaacc agtggctgac ctgccaaacc tggctggcat
     1741 agcgccaggc aaatcccatc cctatcgtcc ccaacacaat accaaagtag cctgccggca
     1801 aattgagcac tttatc
//
LOCUS       DADSYM010000270         1788 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2415
            SAMN08039579-rid11723173.denovo.272, whole genome shotgun sequence.
ACCESSION   DADSYM010000270 DADSYM010000000
VERSION     DADSYM010000270.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039579
            Sequence Read Archive: SRR6362533
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1788)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1788)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980162.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 03:39:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,484
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 4,347
            CDSs (with protein)               :: 4,347
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 43 of 85
            Pseudo Genes (incomplete)         :: 37 of 85
            Pseudo Genes (internal stop)      :: 19 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1788
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039579-rid11723173.denovo.272"
                     /strain="LGE2415"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8302 N 95.9628 W"
                     /collection_date="2015-08-24"
     gene            46..342
                     /locus_tag="J1Y83_004304"
     CDS             46..342
                     /locus_tag="J1Y83_004304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000386949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein SepZ"
                     /protein_id="HBA7632378.1"
                     /translation="MEAANLSPSGAVLPLAATINGNNPVDEKTGVMQSEGGTSRNVRI
                     LSGVLIGAGVLAAIGTGIAAMCVDDPSQRLGLGIAAGVLGGATTVAGGLAMKYA"
     gene            423..800
                     /locus_tag="J1Y83_004305"
     CDS             423..800
                     /locus_tag="J1Y83_004305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312599.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EscI/YscI/HrpB family type III secretion system
                     inner rod protein"
                     /protein_id="HBA7632379.1"
                     /translation="MNNINQSENINIQLNKASQTNVVDEHIPLASTPSAAGAAQFLDQ
                     LLPKTAGVSSPEQVLIEEIKKRHLATMNTDLSFDALSAGGLSPEDVLTLQKNVLNANV
                     NVDVVSKLASLLSTSVTKLVSMQ"
     gene            803..1375
                     /gene="escJ"
                     /locus_tag="J1Y83_004306"
     CDS             803..1375
                     /gene="escJ"
                     /locus_tag="J1Y83_004306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE inner membrane
                     ring protein EscJ"
                     /protein_id="HBA7632380.1"
                     /translation="MKKHIKNLFLLAAICLTVACKEQLYTGLTEKEANQMQALLLSND
                     VNVSKEMDKSGNMTLSVEKEDFVRAITILNNNGFPKKKFADIEVIFPPSQLVASPSQE
                     NAKINYLKEQDIERLLSKIPGVIDCSVSLNVNNNESQPSSAAVLVISSPEVNLAPSVI
                     QIKNLVKNSVDDLKLENISVVIKSSSGQDG"
     gene            1381..>1788
                     /gene="sepD"
                     /locus_tag="J1Y83_004307"
     CDS             1381..>1788
                     /gene="sepD"
                     /locus_tag="J1Y83_004307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE switch protein
                     SepD"
                     /protein_id="HBA7632381.1"
                     /translation="MNNNNGIAKNDCDWLTALDFVKDVNGSPTHLTFYIYQKNAFLHD
                     FGNYWVLYIELSGDFRQVPTDTFIRLCNILAVSKEYKQMGIFLSNKKWYLCQIFHKDN
                     NHRANMSKAIMQHTLASLLDKQFDKLEQSSSSDT"
BASE COUNT          607 a          345 c          357 g          479 t
ORIGIN      
        1 ttataaccag gtaaatgtct aaattagaca aaaggagaaa gatcaatgga agcagcaaat
       61 ttaagccctt ctggtgcagt attaccactg gcagccacaa tcaatggaaa taatccagtg
      121 gatgagaaga caggggtgat gcagtcagaa ggtggaacaa gtcgcaacgt tagaatcctg
      181 agtggagttt taataggtgc aggtgttcta gccgctatcg gtactggtat cgcagcaatg
      241 tgtgttgacg acccttcaca aagattaggc ttaggaatcg cagccggtgt attaggtggc
      301 gcaactactg ttgccggcgg attagcgatg aaatatgcct aatcatcgca tcgcataaaa
      361 atagagaggt aatggatgca ttatgctatt gcctctctca tgaaaagaga ctaacagtaa
      421 atatgaataa tattaatcaa tcggaaaaca ttaatattca gttaaataaa gcatcccaaa
      481 caaatgtcgt tgatgagcat atcccgttag catcaacccc ctctgcagcc ggagcggctc
      541 agtttcttga tcaattactc cctaaaacgg cgggagtgtc ttccccagaa caagtgttga
      601 ttgaagaaat taaaaagcga catcttgcaa caatgaacac cgatctcagt ttcgatgcac
      661 tatctgcagg tgggctctcg cccgaagatg tactcacttt acaaaagaat gtgctcaacg
      721 caaacgtcaa tgttgatgtc gtatctaagt tagcaagcct cctttcaaca tcggttacaa
      781 aattagtttc gatgcaataa aaatgaaaaa acacattaaa aacctttttt tattggctgc
      841 tatttgtttg acagtggcat gtaaagagca attgtataca ggattaacag aaaaagaagc
      901 taatcagatg caagcactgt tgctatccaa tgatgtcaat gtttccaaag aaatggacaa
      961 aagtggaaat atgacactca gtgtggaaaa agaggatttt gttcgcgcaa taacgatttt
     1021 aaataataac ggcttcccga aaaagaaatt tgcagatatc gaggttattt tcccacccag
     1081 ccagttagtg gcatcgccat cacaagaaaa tgcaaaaatc aactatctca aagaacaaga
     1141 catcgaaagg ttgctaagta aaatacccgg tgttatcgat tgcagcgttt cattaaatgt
     1201 taacaataac gaaagtcaac catcctcagc agccgtttta gtcatcagct cgccagaggt
     1261 taacttagca ccatcggtca ttcagataaa aaacctggtt aagaatagtg tcgacgatct
     1321 caaattagaa aatattagtg ttgttatcaa gtcatcctca ggacaggacg ggtaatatgt
     1381 atgaacaata ataatggcat agcaaagaat gattgtgact ggctaacggc ccttgatttt
     1441 gtaaaggatg tcaacggttc accaacgcat ctcacttttt atatctacca aaaaaatgct
     1501 ttcttgcacg attttggaaa ctactgggtt ctatatatcg agcttagtgg tgattttcgt
     1561 caggttccaa cagatacatt tatcagactc tgcaatatcc tggccgttag taaagaatat
     1621 aaacaaatgg gtattttcct gtctaataag aagtggtatc tttgccaaat ttttcataaa
     1681 gataataatc atcgcgcaaa catgtcaaaa gcaataatgc aacatactct cgcctcgttg
     1741 ctcgacaagc aatttgataa actagagcaa tcatcttcat cagataca
//
LOCUS       DADSYM010000271         1783 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2415
            SAMN08039579-rid11723173.denovo.273, whole genome shotgun sequence.
ACCESSION   DADSYM010000271 DADSYM010000000
VERSION     DADSYM010000271.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039579
            Sequence Read Archive: SRR6362533
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1783)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1783)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980162.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 03:39:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,484
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 4,347
            CDSs (with protein)               :: 4,347
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 43 of 85
            Pseudo Genes (incomplete)         :: 37 of 85
            Pseudo Genes (internal stop)      :: 19 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1783
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039579-rid11723173.denovo.273"
                     /strain="LGE2415"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8302 N 95.9628 W"
                     /collection_date="2015-08-24"
     gene            <1..>1783
                     /locus_tag="J1Y83_004308"
     CDS             <1..>1783
                     /locus_tag="J1Y83_004308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415890.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="short-chain fatty acid transporter"
                     /protein_id="HBA7632382.1"
                     /translation="EQASAAARSASAAKTSETNAKASETRAESSKTAAASSASSAASS
                     ASSASASKDEATRQASAAKGSATTASTKATEAAGSATAAAQSKSTAESAATRAETAAK
                     RAEDIASAVALEDASTTKKGIVQLSSATNSTSETLAATPKAVKSAYDNAEKRLQKDQN
                     GADIPDKGRFLNNINAVSKTDFADKRGMRYVRVNAPAGATSGKYYPVVVMRSAGSVSE
                     LASRVIITTATRTAGDPMNNCEFNGFVMPGGWTDRGRYAYGMFWQYQNNERAIHSIMM
                     SNKGDDLRSVFYVDGAAFPVFAFIEDGLSISAPGADLVVNDTTYKFGATNPATECIAA
                     DVILDFKSGRGFYESHSLIVNDNLSCKKLFATDEIVARGGNQIRMIGGEYGALWRNDG
                     AKTYLLLTNQGDVYGGWNTLRPFAIDNATGELVIGTKLSASLNGNALTATKLQTPRLV
                     SGVEFDGSKDITLTAAHVAAFARRATDTYADADGGVPWNAESGAYNVTRSGDSYILVN
                     FYTGVGSCRTLQMKAHYRNGGLFYRSSRDGYGFEEDWAEVYTSKNLPPESYPVGAPIP
                     WPSDTVPSGYALMQGQTFDKSAYPKLAA"
BASE COUNT          475 a          416 c          488 g          404 t
ORIGIN      
        1 ctgaacaggc cagcgcagca gcgaggtctg catccgcagc gaagacatcc gaaacgaacg
       61 cgaaagcgtc ggaaacccgt gcagaatcct caaaaacggc agctgcatcg tccgccagtt
      121 cggcggcgtc atcggcatca tctgcgtctg cttcaaaaga tgaggcgacc agacaggcat
      181 cagcagcgaa aggcagcgcc acgacggcat ccacgaaggc gacagaggca gctggtagtg
      241 cgacggcggc agctcagagc aaaagtacgg cggaatccgc ggcaacgcgc gccgagacag
      301 cagcaaaacg ggcagaggat attgcatccg ccgtggcgct tgaggatgcg agcacgacga
      361 aaaaggggat agtacagctc agcagtgcga ccaacagcac gtctgaaacg ctggcggcaa
      421 cgccaaaggc agtaaaatca gcctatgaca atgcagagaa acgtctgcag aaagaccaga
      481 acggcgctga tatacccgat aagggacgct tcctgaacaa cattaacgcg gtcagtaaaa
      541 cagactttgc tgataagcgt ggtatgcgtt atgtgcgggt taacgctcct gcaggtgcaa
      601 catctggaaa atattaccct gttgttgtta tgcgttctgc tggctcagta agcgaactgg
      661 catcaagggt cattatcacc acggcaacgc gaaccgcagg cgatccgatg aataactgcg
      721 agtttaacgg atttgttatg cctggtggct ggactgacag ggggcgttat gcttatggaa
      781 tgttctggca atatcaaaac aatgaacgag ccatccactc aataatgatg agtaataagg
      841 gcgatgattt gcgctctgtg ttctatgttg atggcgctgc tttccctgtt tttgcgttta
      901 tcgaagatgg cctgtcaata tccgcacctg gtgctgatct cgttgttaat gatacgacct
      961 ataagtttgg ggcaacaaat ccagcgactg aatgtatcgc ggcggacgtt atccttgatt
     1021 ttaagagtgg gcgtggtttt tatgagtctc attcgttaat cgttaacgat aacttgtcgt
     1081 gcaaaaaact ttttgccaca gacgaaattg tagcgcgtgg tggtaatcag attcgaatga
     1141 taggtgggga gtatggtgca ttatggcgta atgatggcgc taaaacttac ctgctgctta
     1201 ccaatcaagg tgatgtttat ggtggctgga atacattaag accgtttgct attgataacg
     1261 caaccggcga actggttatt ggaaccaaac tgtccgcaag tctgaacggt aatgcattaa
     1321 cagcaacaaa gctgcaaacg ccaagactgg tttctggtgt tgagtttgat ggttccaaag
     1381 atattacttt aaccgccgcg catgtggctg cttttgccag aagggcaacg gatacgtatg
     1441 ccgatgcgga tgggggcgtt ccatggaatg ccgaatcagg cgcttacaat gtcacccgct
     1501 ctggcgacag ctatattctg gttaacttct ataccggagt cggaagttgc cggaccctgc
     1561 agatgaaggc acattacaga aatggaggtc tgttctaccg ttcctcaaga gatggctatg
     1621 gttttgagga agactgggca gaagtttata cctcgaaaaa tcttccacca gaaagctacc
     1681 cagtcggcgc accaatcccg tggccatcag ataccgttcc gtctggctat gccctgatgc
     1741 aggggcagac ttttgacaaa tctgcatacc cgaaacttgc agc
//
LOCUS       DADSYM010000272         1776 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2415
            SAMN08039579-rid11723173.denovo.274, whole genome shotgun sequence.
ACCESSION   DADSYM010000272 DADSYM010000000
VERSION     DADSYM010000272.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039579
            Sequence Read Archive: SRR6362533
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1776)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1776)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980162.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 03:39:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,484
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 4,347
            CDSs (with protein)               :: 4,347
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 43 of 85
            Pseudo Genes (incomplete)         :: 37 of 85
            Pseudo Genes (internal stop)      :: 19 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1776
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039579-rid11723173.denovo.274"
                     /strain="LGE2415"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8302 N 95.9628 W"
                     /collection_date="2015-08-24"
     gene            complement(<1..505)
                     /locus_tag="J1Y83_004309"
     CDS             complement(<1..505)
                     /locus_tag="J1Y83_004309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBA7632383.1"
                     /translation="MTETESAILAHARRCAPAESCGFVVRTPEGESYFPCVNISGEPE
                     DYFRMAPEDWLQAEMQGEIVALVHSHPSGLPWLSEADRRLQVQSDLPWWLVCRGAIHK
                     FRCVPHLTGRRFEHGVTDCYALFRDAYHLAGTELPDFHREDDWWRHGQNLYLDNMEAT
                     GFYRVALT"
     gene            complement(510..1208)
                     /locus_tag="J1Y83_004310"
     CDS             complement(510..1208)
                     /locus_tag="J1Y83_004310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HBA7632384.1"
                     /translation="MQDIRQETLNECTRAEQSASVVLWEIDLTEVGGERYFFCNEQNE
                     KGEPVTWQGRQYQAYPIQGSGFELNGKGTSTRPTLAVSNLYGMVTGMVEDMQSLVGGT
                     VVRRKVYARFLDAVNFVSGNSNADPEQEVISRWRIEQCSELSAVSASFVLATPTETDG
                     AVFPGRTMLANTCTWTYRGDECGYNGPAVADEYDQPTSDITKDKCSKCLSGCKFRNNV
                     GNFGGFLSINKLSQ"
     gene            complement(1208..1537)
                     /locus_tag="J1Y83_004311"
     CDS             complement(1208..1537)
                     /locus_tag="J1Y83_004311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBA7632385.1"
                     /translation="MKTFRWKVKPGMDVTSAPSVREVRFGDGYSQRAPAGLNADLKTY
                     SVTLSVSREEARALESFLAEHGGWKAFLWTPPYGWRQIRVTCAKWTSRVSMLRVEFSA
                     EFKQVVN"
     gene            complement(1534..>1776)
                     /locus_tag="J1Y83_004312"
     CDS             complement(1534..>1776)
                     /locus_tag="J1Y83_004312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HBA7632386.1"
                     /translation="LMRGYATGGYVGTPGSLADSRSQASGKFEQNNHVVINNDGTNGQ
                     IGPQALKAVYDMARKGARDEIQAQMRDGGLFSGGGR"
BASE COUNT          378 a          595 c          434 g          369 t
ORIGIN      
        1 ctgtcagtgc gacacggtaa aaacccgtcg cctccatatt gtcaagatag agattctgac
       61 cgtgacgcca ccagtcatcc tcccggtgaa aatccggcag ttcagtcccc gccagatgat
      121 aagcgtcccg gaacagcgcg taacagtccg tcaccccgtg ctcaaagcgc cgcccggtga
      181 gatgcggcac acagcggaac ttatgaatcg ccccccggca gaccagccac cacggcaaat
      241 cactctgcac ctgcagccgc cggtcagcct cactcagcca gggcagacca ctggggtggc
      301 tgtggaccag cgccacaatc tcaccctgca tttctgcctg cagccagtcc tccggagcca
      361 tccggaaata atcctccggc tcaccggaaa tattcacgca gggaaaatag ctttccccct
      421 ccggcgttct caccacgaag ccgcacgact ccgctggcgc acatcgccgg gcgtgcgcca
      481 gaatcgctga ttctgtctct gtcatgggat tactgcgaaa gtttgttaat ggaaaggaaa
      541 ccgccaaagt tgccgacgtt attgcggaac ttacagccgc tcaggcattt gctgcattta
      601 tccttcgtta tatcggacgt cggctggtca tattcatccg cgacagccgg accgttataa
      661 ccgcactcat caccgcgata ggtccaggtg caggtgttgg ccagcatggt acgtcccgga
      721 aaaacagcgc catccgtttc cgtcggcgtg gccagtacaa aagaggcact gactgcgctc
      781 agttcgctgc actgctcgat gcgccagcgg ctgatcacct cctgctccgg atcggcgttg
      841 ctgtttccgc tgacgaagtt caccgcatcc agaaaacggg cgtaaacctt acgccggact
      901 accgttccgc cgaccagact ctgcatatct tccaccatac cggtgaccat gccgtacagg
      961 ttagagactg ccagcgtggg ccgcgtactg gtgcctttgc cattcagttc aaaaccgctc
     1021 ccctgaatgg ggtatgcctg atactgtcgc ccctgccagg tgaccggctc acctttttcg
     1081 ttctgctcat tacagaaaaa ataacgttct ccaccaacct ctgtcagatc gatttcccag
     1141 agcaccacgc tggccgactg ctccgcacgg gtgcattcat tcagtgtttc ctgccggata
     1201 tcctgcatca gttcaccacc tgtttaaact ctgcgctgaa ctcaacacgt aacatactga
     1261 cccgcgatgt ccattttgcg caggtcaccc tgatctgccg ccagccataa ggcggcgtcc
     1321 acagaaaggc tttccagccc ccgtgctcag ccagaaacga ctccagcgcc ctggcctcct
     1381 cgcgggagac agaaagcgtc acgctgtacg ttttcaggtc agcattcagc ccggcaggcg
     1441 cacgctggga atagccatca ccaaagcgca cctccctgac ggaaggagcc gatgtcacat
     1501 ccatcccggg tttcactttc cagcggaagg ttttcatcgt ccacctccgg agaacaagcc
     1561 accatcacgc atctgtgcct gaatttcatc acgggcaccc ttgcgggcca tgtcatacac
     1621 agccttcagc gcctgtggtc caatctgccc gttcgtgccg tcgttgttaa tcaccacatg
     1681 gttattctgc tcaaacttcc cggacgcctg cgaccggctg tccgccagac tgcccggtgt
     1741 accgacataa ccgccggtgg catagccgcg cataag
//
LOCUS       DADSYM010000273         1731 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2415
            SAMN08039579-rid11723173.denovo.275, whole genome shotgun sequence.
ACCESSION   DADSYM010000273 DADSYM010000000
VERSION     DADSYM010000273.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039579
            Sequence Read Archive: SRR6362533
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1731)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1731)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980162.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 03:39:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,484
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 4,347
            CDSs (with protein)               :: 4,347
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 43 of 85
            Pseudo Genes (incomplete)         :: 37 of 85
            Pseudo Genes (internal stop)      :: 19 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1731
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039579-rid11723173.denovo.275"
                     /strain="LGE2415"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8302 N 95.9628 W"
                     /collection_date="2015-08-24"
     gene            complement(29..1729)
                     /locus_tag="J1Y83_004313"
     CDS             complement(29..1729)
                     /locus_tag="J1Y83_004313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000583409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HscC"
                     /protein_id="HBA7632387.1"
                     /translation="MIIGIDLGTTNSLAAVWRNGQSELIPNALGKFLTPSVVCVDEDG
                     MVLTGEAARDLQLIKPQNCASNFKRIMGTSKTLKLGGREFRAEELSSLILRQLKEDAE
                     NYLGEEVTEAVISVPAYFGDMQRKATKAAATMAGLNVERLINEPTAAALAYGLHNKDD
                     EHQFLVFDLGGGTFDVSILELFDNIMEVRASAGDNFLGGEDIVDILIDAYCSRRDLPE
                     NIEWREPTLQRHLRIEAERVKRVLSVRDEATFSVEIEGRRYYWHLTTEKFEFLLQTFF
                     ERIHMPLERAIRDAKINISQLDQVVLVGGTTRMPLIRKLVTRLFGRIPAMHLNPDEVI
                     AQGAAIQAALKARHSELRDVVVTDVCPYTLGVDTSKSLGHTRESGYFAPVIERNRSIP
                     CSRVRAFYTAHDQQTEVNFKIYQGESRMVADNIFLAELSVNVPPKPAGDVRIDVRFTY
                     DINGILDVDISVPLTGAKNSLVIEQNPGALTAEQIQQSLSKLSLLKIHPRDEQINQAF
                     IARLDNLYQLTLAETRDWISNCSRHFSYLLEKQDPDQLADFRTKVEPTLDSLERERLQ
                     "
BASE COUNT          471 a          422 c          354 g          484 t
ORIGIN      
        1 ggttgtaaag ctaatatttc ccagcagttc attgcagacg ttctctttcc agagagtcta
       61 atgtaggttc aactttagtc cgaaaatcag caagttgatc aggatcctgc ttctcgagta
      121 gataactgaa atgacgggag caattgctta tccagtctcg cgtttccgcc aaggtgagtt
      181 gatagagatt atcaagacgg gcaataaagg cctggtttat ctgttcatcg cgtgggtgaa
      241 tttttaacag gcttaatttg gacaacgact gctgtatttg ttctgccgtt aatgcccctg
      301 gattctgttc aataaccaga ctatttttag cacctgtcag tggcacgctg atatcgacat
      361 caagaatacc gttaatgtcg tacgtaaaac gaacatcaat acgcacatca ccggccggtt
      421 ttggcggaac attcactgac aactccgcaa ggaaaatatt atcagccacc atcctgcttt
      481 caccctggta gattttaaaa ttaacctctg tttgttgatc atgtgccgtg taaaaggcac
      541 gaacgcgact acaaggaata ctcctgttac gctcaataac gggtgcaaaa tagcctgatt
      601 cgcgggtatg ccccagcgat ttagatgtat caacgccaag cgtataaggg caaacatcag
      661 tcactacaac atctcgtaat tcgctgtgcc gcgctttaag tgctgcctga atagccgcgc
      721 cctgggcaat gacttcatcc gggtttaaat gcattgccgg tatacgacca aataatcgtg
      781 tcacaagttt gcgaatcagt ggcatacgtg tagtgccacc cacaagaaca acctggtcta
      841 attggctaat attaattttg gcatcgcgaa ttgcccgctc cagtggcata tggattcgtt
      901 cgaaaaaggt ctgtaataaa aattcaaatt tttctgtagt cagatgccag taatatcgcc
      961 ttccttcaat ctccacactg aaagtggctt catctcgcac actcagcacg cgtttgacac
     1021 gctcagcctc gatgcgtaaa tgccgctgga gtgtcggttc acgccattca atattttcag
     1081 gaagatcccg acgtgagcaa tatgcatcga tcaggatatc gacaatatct tcaccaccta
     1141 aaaagttatc cccggcactg gcacgtactt ccataatatt gtcaaacaac tccagtatgg
     1201 agacatcaaa cgtaccacca cctaaatcga ataccagaaa ttgatgttca tcatccttat
     1261 tatgcaaacc atacgccagt gcagcggcgg ttggttcgtt aattaaacgt tcgacattta
     1321 agcctgccat tgtcgcagcg gcttttgttg ctttacgttg catatcacca aaatatgcgg
     1381 gaacgctaat caccgcttca gtgacttctt cgcccaaata gttttctgca tcttctttta
     1441 gctgacgtaa tatcaacgat gagagctctt ccgcacgaaa ttcccgtccg cccagtttta
     1501 acgtctttga tgtgcccata atgcgtttaa aatttgatgc gcagttttgt ggtttgatga
     1561 gttgcaggtc acgagctgcc tcccctgtca aaaccatacc atcttcgtca acacaaacta
     1621 cgctgggtgt taaaaactta cctaacgcat tggggataag ttcactttgt ccatttcgcc
     1681 agacagctgc caaactgtta gttgtaccca ggtcgatacc gataatcata a
//
LOCUS       DADSYM010000274         1731 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2415
            SAMN08039579-rid11723173.denovo.276, whole genome shotgun sequence.
ACCESSION   DADSYM010000274 DADSYM010000000
VERSION     DADSYM010000274.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039579
            Sequence Read Archive: SRR6362533
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1731)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1731)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980162.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 03:39:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,484
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 4,347
            CDSs (with protein)               :: 4,347
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 43 of 85
            Pseudo Genes (incomplete)         :: 37 of 85
            Pseudo Genes (internal stop)      :: 19 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1731
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039579-rid11723173.denovo.276"
                     /strain="LGE2415"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8302 N 95.9628 W"
                     /collection_date="2015-08-24"
     gene            complement(93..1712)
                     /locus_tag="J1Y83_004314"
     CDS             complement(93..1712)
                     /locus_tag="J1Y83_004314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001489516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter"
                     /protein_id="HBA7632388.1"
                     /translation="MVVSLTSVLTRLSPVQIPLPLIQIAAGAVLAQPIFGLHVEFNPE
                     LFLLLFIPPLLFAESSKIQPKELIKHSREIISLALVLVLITIFGVGYVIHLLLPNVPL
                     IAAFALAAVLSPTDAVALLGIVGKGRISKNIQEVLEGEALMNDASGLVALKFAVAVTM
                     GTMEFSVHGATIAFFVVALGGIAVGIAVTWLYGKGLLLISRYAHDEPSIQIVLMLLLP
                     FIVYLVAEHFGLSGILAAVAAGLTTSRIGVVHHAPIRLRIKAKSSWSMLEYVFNGMVF
                     LILGLQLPGIISESLIRAENDPLVDTGSLLLDVIWIYLALMVVRLGWLWLMRLYSRKR
                     PGKHPMLFAGYKTRHMLLATFAGVRGAITLAGVLSIPLLLPDGTPFPGRYLLVFLATG
                     VILFSLVCGIIILPLLLGKNVGIFSDNRQKEEEAADLFMAKMAILSVQKTEQRLLRAR
                     TENLDNELIMETGARVIGYMRHSEEDMSPNDNSLHMQRAKLERTFNLAALNAKRAAIY
                     QMRARQEVSDETLAKIVSELDVLETLIMDSR"
BASE COUNT          509 a          450 c          374 g          398 t
ORIGIN      
        1 ttctgcccat aaggaaatac atagggaata aacgatgctg gaagtattac tctcagccat
       61 catttaattc cctattactg gcagcgataa aattatcggc tatccatgat taacgtttcc
      121 agcacatcta attcactgac tatttttgcc agtgtttcat cactgacttc ttgccttgcg
      181 cgcatctggt aaatagccgc gcgtttggca ttcaacgccg ccagattaaa tgttctttcc
      241 agcttcgcac gctgcatatg cagggaatta tcattaggcg acatatcctc ttcactatgt
      301 cgcatataac cgataactct ggcccccgtt tccataatca actcgttatc gaggttttct
      361 gttctggcgc gcagcaaacg ttgctcggtc ttttgcacac tcagtatggc cattttagcc
      421 ataaacagat cggcagcttc ctcttctttt tgtcggttat cgctgaaaat ccccacattt
      481 ttccccagta ataacggcag aataataatg ccgcaaacca gcgaaaagag gatcacacct
      541 gtggcgagaa ataccagcaa gtaacgcccg ggaaaaggtg tgccatccgg cagcaataac
      601 gggatagaaa gcacccccgc aagcgtgata gctccgcgca cccccgcaaa ggtcgccagc
      661 agcatatgac gcgttttata tccagcaaac agcatcggat gtttgccggg tcgtttacgg
      721 ctatacagac gcatcagcca aagccagccc aggcgcacaa ccattaacgc caggtagatc
      781 caaatgacat caaggagcag cgatccggtg tccaccagtg gatcgttttc tgcacggatc
      841 agtgattccg aaataatgcc cggcagttgt aagcctaaaa tcagaaacac catgccgtta
      901 aagacatatt caagcatcga ccagctactt ttagctttaa ttctcaggcg aataggcgcg
      961 tggtggacta cgccaatgcg actggttgtt aaccccgcgg cgacagcggc aagaatgcca
     1021 gataagccaa aatgttccgc aaccagataa acaataaacg gcaacagaag catcaataca
     1081 atctgaatgg aaggttcatc gtgagcatat cggctgatca acagcagccc tttaccatat
     1141 aaccaggtaa cagcgatccc cacggcaata ccgcccaacg caacgacaaa aaacgcaatg
     1201 gttgcgccat gaacgctgaa ttccatcgtt cccatcgtca ctgcaacagc aaatttcaac
     1261 gccaccaggc cggaagcatc attcatcaac gcttcgcctt cgagcacttc ctggatgttt
     1321 ttgctgatac gccctttccc gacaatcccc agcaacgcca cggcatcggt aggcgacaac
     1381 acggcggcta atgcgaaagc agcgatgagc ggcacattcg gtaagagtaa atgaatcaca
     1441 tagccgacac caaaaatagt gattagtacc aacacaagtg ccaggctgat aatttcccgg
     1501 ctgtgcttga tcaattcttt aggttgtatt tttgaacttt cagcaaacaa caggggtggt
     1561 ataaacaata agagaaaaag ttcaggattg aattccacat gcagaccaaa tataggctgg
     1621 gccagtactg caccagcggc aatctggatt aaaggaagtg gtatctggac aggagacaga
     1681 cgtgtaagca cgctggttaa tgatactacc aacgtaagaa ttaaaattgt t
//
LOCUS       DADSYM010000275         1692 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2415
            SAMN08039579-rid11723173.denovo.277, whole genome shotgun sequence.
ACCESSION   DADSYM010000275 DADSYM010000000
VERSION     DADSYM010000275.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039579
            Sequence Read Archive: SRR6362533
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1692)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1692)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980162.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 03:39:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,484
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 4,347
            CDSs (with protein)               :: 4,347
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 43 of 85
            Pseudo Genes (incomplete)         :: 37 of 85
            Pseudo Genes (internal stop)      :: 19 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1692
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039579-rid11723173.denovo.277"
                     /strain="LGE2415"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8302 N 95.9628 W"
                     /collection_date="2015-08-24"
     gene            160..375
                     /gene="essD"
                     /locus_tag="J1Y83_004315"
     CDS             160..375
                     /gene="essD"
                     /locus_tag="J1Y83_004315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416074.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage lysis protein EssD"
                     /protein_id="HBA7632389.1"
                     /translation="MKSMDKLTTGVAYGTSAGNAGFWALQLLDKVTPSQWAAIGVLGS
                     LVFGLLTYLTNLYFKIKEDRRKAARGE"
     gene            380..691
                     /locus_tag="J1Y83_004316"
     CDS             380..691
                     /locus_tag="J1Y83_004316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1327 domain-containing protein"
                     /protein_id="HBA7632390.1"
                     /translation="MTQDYELVVKGVRNFENKVTVTVALQDKERFDGEIFGLDVAMDR
                     VEGAALEFYEAAARMSVRQVFLEVAEKLSEKVESYLQHQYSFKIENPANKHERPHHKY
                     L"
     gene            688..1221
                     /locus_tag="J1Y83_004317"
     CDS             688..1221
                     /locus_tag="J1Y83_004317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HBA7632391.1"
                     /translation="MNTKIRYGLSAAVLALIGAGASAPQILDQFLDEKEGNHTMAYRD
                     GSGIWTICRGATVVDGKTVFPNMKLSKEKCDQVNAIERDKALAWVERNIKVPLTEPQK
                     AGIASFCPYNIGPGKCFPSTFYKRLNAGDRKGACEAIRWWIKDGGRDCRIRSNNCYGQ
                     VIRRDQESALTCWGIEQ"
     gene            1218..>1692
                     /locus_tag="J1Y83_004318"
     CDS             1218..>1692
                     /locus_tag="J1Y83_004318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2514 domain-containing protein"
                     /protein_id="HBA7632392.1"
                     /translation="MNQIFMVIFLVLSGFIVGNVWSDRGWQKKWAERDAAALSQEVNA
                     QFAARIIEQGRTIARDEAVKDAQQKSAEISARAAYLSDSVNQLRAEAKKYAIRLDAAK
                     HTADLAAAVRGKTTKTAEGMLTNMLGDIAAEAQLYAEIADERYIAGVTCQQIYESL"
BASE COUNT          477 a          349 c          432 g          434 t
ORIGIN      
        1 tattatttta tatatgactt tctttcaaaa taattactca catttttaat gtgtatgttt
       61 ctttagcgcc gttgagaaca acatgtgctg tcaaaactac cccgtagact ccgatctttt
      121 caaacatatt gcaccatccg tgtacatcgg ggtgaggata tgaaatcaat ggataagtta
      181 acaacaggtg ttgcctatgg cacatcggcg ggtaatgctg gtttctgggc attgcagtta
      241 ctcgataaag taactccgtc acagtgggct gcaatcggtg tgctgggtag cctggttttt
      301 ggcctgctga cgtatctgac aaatctttat ttcaagatta aagaagacag gcgtaaggct
      361 gcgagaggag agtaatccaa tgactcaaga ctatgaactg gttgtgaaag gagtccgtaa
      421 ttttgagaat aaagttacgg taactgtagc cttacaggac aaagaacgct ttgacggtga
      481 aatttttggc ctggatgtcg ccatggaccg tgttgaagga gctgcgctgg agttttatga
      541 ggcagcagcc agaatgagcg tccggcaagt cttcctggaa gtagcagaaa aattgtcaga
      601 aaaagttgag tcttatctgc agcatcagta ctcctttaag attgaaaatc ctgccaataa
      661 gcacgagcgt cctcatcata aatatctatg aacacaaaaa tcagatacgg cctgtcggct
      721 gccgttctgg cgctgattgg tgctggcgca tctgctcctc agatacttga ccagtttctg
      781 gacgaaaaag aaggtaacca cacaatggca taccgcgatg gttctggcat atggaccatc
      841 tgtcggggtg ccacagtggt ggatggaaaa accgtttttc ccaatatgaa actgtcgaag
      901 gaaaaatgcg accaggtcaa cgccattgag cgtgataagg cgctggcatg ggtggagcgc
      961 aatattaaag taccactgac cgaaccacaa aaagcgggta tcgcgtcatt ttgtccctat
     1021 aacattggcc ccggtaagtg tttcccgtcg acgttttata agcggctgaa tgctggtgat
     1081 cgtaaaggtg catgcgaagc gattcgctgg tggattaagg atggcggacg cgattgccgc
     1141 attcgttcaa ataactgtta cggtcaggtt attcgtcgtg accaggagag cgcattaacc
     1201 tgctggggga tagaacagtg aatcagatat tcatggtgat ttttctcgtg ttgtcaggat
     1261 ttatcgtcgg aaatgtctgg agcgacagag gatggcaaaa aaaatgggcg gaacgtgatg
     1321 ctgccgcatt atcacaagag gtaaatgctc aatttgctgc tcgaataatt gaacaggggc
     1381 gaactatagc ccgtgatgag gctgttaaag atgcacaaca gaaatctgct gaaatttctg
     1441 ccagggctgc ttatctgtct gatagtgtta accagttgcg tgccgaagca aaaaaatatg
     1501 ccatacgcct tgacgcagcg aagcataccg cagatcttgc cgctgccgtc agaggcaaaa
     1561 caaccaaaac cgccgaagga atgctcacca acatgctcgg agatattgca gcagaagctc
     1621 agctttatgc tgaaattgct gacgaacgct acatcgcagg agtgacttgt caacagatct
     1681 atgaatcttt aa
//
LOCUS       DADSYM010000276         1689 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2415
            SAMN08039579-rid11723173.denovo.278, whole genome shotgun sequence.
ACCESSION   DADSYM010000276 DADSYM010000000
VERSION     DADSYM010000276.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039579
            Sequence Read Archive: SRR6362533
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1689)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1689)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980162.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 03:39:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,484
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 4,347
            CDSs (with protein)               :: 4,347
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 43 of 85
            Pseudo Genes (incomplete)         :: 37 of 85
            Pseudo Genes (internal stop)      :: 19 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1689
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039579-rid11723173.denovo.278"
                     /strain="LGE2415"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8302 N 95.9628 W"
                     /collection_date="2015-08-24"
     gene            complement(234..743)
                     /locus_tag="J1Y83_004319"
     CDS             complement(234..743)
                     /locus_tag="J1Y83_004319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001592936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector protein EspH"
                     /protein_id="HBA7632393.1"
                     /translation="MSSSLSGITFTTFLTSNHASWNKLTRTLHISNDDILDELQLKAR
                     MQQRHHRVFAGIDDYSIINFRGKDYAVRFIKDNDNYVYKVNKITQESGCFSKIFSFLY
                     GGVTKALESKLNERHITPLSSTWFPRTALEGFLTERGLSSLLRRVQSTEGVNPPENTT
                     LNSLSGVTV"
     gene            complement(774..1682)
                     /gene="escQ"
                     /locus_tag="J1Y83_004320"
     CDS             complement(774..1682)
                     /gene="escQ"
                     /locus_tag="J1Y83_004320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001487583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE ring protein EscQ"
                     /protein_id="HBA7632394.1"
                     /translation="MSSQLNMKINDFYLPLLPVIGTGRLYITSEGHACHAYFREVSGH
                     GIRFTLTYSGYEGRFWISEEQFIQWCQELFPYSESRLIPEDMIKLMILWVMQTALPEG
                     DVSVDDVQFTMLNKDVYPVIENNNGENRLNVIILETTVQSLQHLINDNWQFVPHSNAL
                     FFDGYIVPGWTDYPVTELRVGDSLRLYHVDDSQERRCWIVINNPLATVKLSDNNLSVT
                     DVLAADPLCTISSETVMNRIYCVIGTVHIDIHMLRNVQKDDIIDSTGYHLFGGCQLVR
                     NNTTIAYGSIIKINEDFYFTISVVCD"
BASE COUNT          546 a          359 c          279 g          505 t
ORIGIN      
        1 aaaaaagaaa taataaaact actttcactt tgatttattt gctttttact ttggaagtga
       61 taatatggac tggacggtgg catccattca gttcatattt cacaaaaact acagtgctct
      121 atccttgaaa tactaatcat ttcgcctgca tattcttttc aaaaaataat gaaatatcgc
      181 ccccagaaca gaacacattc tggggagaaa aagaaaacag cataaaataa tacttaaact
      241 gtcacacctg ataaagagtt taatgtagta ttctctggag ggttcacacc ttctgtagac
      301 tgtacgcgac gtaacaaaga agagagccct ctttcagtta aaaagccttc cagtgcagtg
      361 cgaggaaacc aggttgaaga aagtggcgta atgtgtctct catttaattt actttccagg
      421 gccttcgtaa cgcctccata caaaaagcta aatatctttg agaaacatcc actttcctga
      481 gttatcttat ttactttgta tacataatta tcattatctt taatgaagcg gacagcataa
      541 tcttttccac gaaaattgat aattgaatag tcatcaatgc ctgcaaaaac ccggtggtga
      601 cgctgctgca tgcgtgcttt aagttgcagt tcatctagta tatcgtcatt cgatatatga
      661 agagttcggg tcaacttatt ccatgacgca tgattgcttg tcaaaaaagt agtgaatgtt
      721 atacctgata atgatgacga catcatacac ctccctatat aacgcattcc tgattaatca
      781 catactacgc taatagtaaa ataaaaatct tcgttgattt ttataatact cccgtatgcg
      841 attgtggtat tattccgtac taactggcac ccaccaaaaa gatgatatcc cgttgaatcg
      901 ataatgtcat ctttttggac attacgcaac atatgtatat caatatgcac tgtgccaatc
      961 acacaataaa tgcgattcat cacagtctcg ctgctgatag tacataacgg atctgcagcc
     1021 agaacatccg tgacagataa attattatcg ctcagcttta cggttgccaa agggttatta
     1081 ataacaatcc agcaccttct ctcttgacta tcatcaacat gatagagtct cagactatca
     1141 ccaactctca actctgtaac tggataatca gtccacccag gtacgatata gccatcaaaa
     1201 aaaagagcat tagaatgtgg aacaaattgc caattatcat taatcagatg ttgtaaactt
     1261 tgtaccgttg tttcgagtat aattacatta agtctgtttt ctccattatt attttctatt
     1321 accggataaa catctttatt tagcatcgta aattgaacat catcaactga cacatcacct
     1381 tccggtaagg cagtctgcat tacccataag atcatgagtt ttatcatatc ttccggtatc
     1441 agccggcttt cgctatacgg gaataactct tggcaccact gaatgaactg ttcttctgat
     1501 atccagaaac gcccctcata gcccgagtat gtcaacgtga aacgaatacc atgaccgctg
     1561 acctcacgaa aatatgcatg gcaagcatga ccttcggacg taatatagag gcgaccggtt
     1621 ccgataactg gcaacaacgg caaataaaaa tcgttaattt tcatattcaa ttgtgaactc
     1681 aatggcttc
//
LOCUS       DADSYM010000277         1684 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2415
            SAMN08039579-rid11723173.denovo.279, whole genome shotgun sequence.
ACCESSION   DADSYM010000277 DADSYM010000000
VERSION     DADSYM010000277.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039579
            Sequence Read Archive: SRR6362533
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1684)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1684)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980162.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 03:39:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,484
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 4,347
            CDSs (with protein)               :: 4,347
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 43 of 85
            Pseudo Genes (incomplete)         :: 37 of 85
            Pseudo Genes (internal stop)      :: 19 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1684
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039579-rid11723173.denovo.279"
                     /strain="LGE2415"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8302 N 95.9628 W"
                     /collection_date="2015-08-24"
     gene            30..1136
                     /locus_tag="J1Y83_004321"
     CDS             30..1136
                     /locus_tag="J1Y83_004321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001489581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent flavin oxidoreductase"
                     /protein_id="HBA7632395.1"
                     /translation="MLTEKIKLRNRIVMAPMTTWSANPDGTISEQELEFYKRRSQNVG
                     LVITGCTYVTPSGIGFTHEFAAYDDRFINSLEKLAAAAKSGGAPAILQIFHAGNKAIP
                     ELVPNNDVISASASSVKSGDFMKRVVQSREMTENEIQETIRAFGDVTKRAIKAGFDGV
                     ELHGAHGFLLQNFFSPLFNQRNDRWGGDLEGRMRFPLAVLQEVKNVVYEYATKPFAIG
                     YRISPEESVTGGLRIEDTYKLLDRLISSGISYIHTSLVSINDSYPVESPNGPRTIELI
                     LNHIAGRVPVIAAGKIRTPSQAQEAISAGLPLVAIGKGLVINPEWVTLAESGRSHEIQ
                     TALNPQRVPELTIPDKLWDQIQASKGTGWFPLMD"
     gene            1609..>1684
                     /locus_tag="J1Y83_004322"
     CDS             1609..>1684
                     /locus_tag="J1Y83_004322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA7632396.1"
                     /translation="MKENKVQQISHKLINIVVFVAIVEY"
BASE COUNT          507 a          362 c          363 g          452 t
ORIGIN      
        1 ataaacaccc atcattgttt tctcctttta tgctgacaga aaaaataaag ctacgtaatc
       61 gtatcgttat ggctcctatg acaacctggt cagctaatcc tgatggaacc atctctgagc
      121 aagaactcga attttataaa cgtcgctcac aaaatgttgg tttagtaata acaggatgca
      181 cctatgtcac gccaagcggc attgggttta ctcatgaatt tgccgcgtat gacgaccgtt
      241 tcattaacag ccttgaaaag ttagcagctg cagccaaaag tggcggtgct ccggctattt
      301 tgcagatttt ccacgccggt aataaagcca tcccggagct tgtgccaaat aacgatgtta
      361 ttagtgccag cgcatcaagc gtaaaatctg gtgactttat gaagagagtg gtacaaagca
      421 gggaaatgac tgaaaacgaa attcaggaaa ccattagagc attcggagat gtcacaaaaa
      481 gggcgattaa agcaggtttt gatggcgttg aacttcatgg tgcgcacgga ttccttctgc
      541 agaatttctt ctcccctcta tttaaccagc gtaatgaccg atggggagga gatcttgaag
      601 gacgtatgcg cttcccgctg gccgtattac aggaagtaaa aaatgtagtc tatgaatatg
      661 cgacaaagcc ctttgctatc ggttacagaa tatctcctga agaatctgta acaggggggc
      721 tgcggataga ggatacttat aaacttcttg atcgactcat ttcatccggt atttcgtata
      781 tacatacctc gctggtcagt attaacgata gctaccctgt tgagtcgcca aatggccccc
      841 gaactattga attgatttta aatcatattg caggcagagt gccggtaatc gccgcaggta
      901 aaatacgcac accgtctcag gctcaagaag caatttccgc tggcctgcca cttgttgcga
      961 tcggtaaagg gctggtgata aatccagaat gggtgacttt agctgaatca ggtagaagtc
     1021 atgaaattca aacagccctt aatccacaac gcgttcctga attaaccatt ccggataaac
     1081 tatgggatca aattcaggca tcgaaaggaa ctggctggtt tccgttaatg gattaaatat
     1141 cgattactct cccgagagta aaactatcaa atcaatgaat aaccgataca gcctcgcaaa
     1201 ttctttcaga taattaaccc gtcactgcgg cacgttctct cgcaaggcgt gcctgcagtg
     1261 taatccagta tgtatccggc atagttgacg gtttaataga ctcaattcct ctccctggtt
     1321 acctacaacg atatcttgta taacacccca ggagatacga cgttacgatg agatgcgata
     1381 tgctcctcag cgccaatcaa cgctaggact gggccagtct tgagatcaga ctcattcccg
     1441 taactgattt caggcgacat atatcaatga cgttggggca ataaaagata gccgatgtaa
     1501 tacatctatt caaatttgtg atacatgtca aaaaattggt tgaccaaact cgttatttat
     1561 ataagggcac ttacgaagtg cactcttttt taaagcgagg aagtaccaat gaaagagaat
     1621 aaagtacagc aaatcagtca taaactgatt aatatcgttg tttttgtcgc aattgtagaa
     1681 tacg
//
LOCUS       DADSYM010000278         1647 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2415
            SAMN08039579-rid11723173.denovo.280, whole genome shotgun sequence.
ACCESSION   DADSYM010000278 DADSYM010000000
VERSION     DADSYM010000278.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039579
            Sequence Read Archive: SRR6362533
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1647)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1647)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980162.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 03:39:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,484
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 4,347
            CDSs (with protein)               :: 4,347
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 43 of 85
            Pseudo Genes (incomplete)         :: 37 of 85
            Pseudo Genes (internal stop)      :: 19 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1647
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039579-rid11723173.denovo.280"
                     /strain="LGE2415"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8302 N 95.9628 W"
                     /collection_date="2015-08-24"
     gene            complement(157..654)
                     /gene="rayT"
                     /locus_tag="J1Y83_004323"
     CDS             complement(157..654)
                     /gene="rayT"
                     /locus_tag="J1Y83_004323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="REP-associated tyrosine transposase RayT"
                     /protein_id="HBA7632397.1"
                     /translation="MSEYRRYYIKGGTWFFTVNLQNRRNQLLTTQFQTLRNVIIKVKR
                     DRPFEINAWVVLPEHMHCIWTLPEGDDDFSSRWREIKKQFTHACGLKNIWQPRFWEHA
                     IRNTKDYRHHVDYIYINPVKHGWVKQVSDWPFSTFHRDVAKGLYPIDWAGDVTDFSAG
                     ERIIL"
     gene            complement(830..1579)
                     /locus_tag="J1Y83_004324"
     CDS             complement(830..1579)
                     /locus_tag="J1Y83_004324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000093946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HBA7632398.1"
                     /translation="MSSFLHGRFLHPGVFSLCVLLPLLASATTSHISFSYAARQRMQN
                     RARLLKQYQTHLKKQASYIVEGNAKSRRALRQHNREQIKQHPEWFPAPLKASDRRWQA
                     LVENNHFLSSDHLHNITEVAIHRLEQQLGKPYIWGGTRPDKGFDCSGLVFYAYNKILE
                     AKLPRTANEMYHYRRATIVANNDLRRGDLLFFHIHSREIADHMGVYLGDGQFIESPRT
                     GETIRVSRLAEPFWQDHFLGARRILTEETIL"
BASE COUNT          434 a          405 c          403 g          405 t
ORIGIN      
        1 cgcggttttg tcggcatgat aagacgcgta gcgtcgcatc tggcatcggc gtacaactgc
       61 cggatgcggc gtacatgcct tatccggcct acgggtgagc gcggttttgt aggcatgata
      121 agacgcgtct gcgtcgcatc aggcatcggc gcacagctat aaaatgatac gctccccagc
      181 actaaaatcc gttacgtctc ccgcccaatc gatgggatat aaccctttcg caacatcgcg
      241 gtgaaacgtt gagaatggcc aatcactcac ttgttttacc caaccatgtt ttactggatt
      301 tatataaata taatcaacat gatggcgata gtctttggta ttgcggatag catgttccca
      361 aaaacgtggt tgccagatat ttttcaatcc acaagcatgg gtaaattgct ttttaatttc
      421 ccgccagcgc gaggaaaaat catcatcgcc ttcaggtaat gtccatatgc aatgcatatg
      481 ctccggcaaa actacccaag cgttaatttc aaaaggtctg tctcgcttaa ctttaataat
      541 aacgttacgg agcgtctgaa actgggtggt caaaagttgg tttcgacgat tctgtaaatt
      601 taccgtgaaa aaccaggttc cccccttgat gtaataacga cgatattcag acataacgac
      661 tcctttttct caacggtaag aagaaaagga aaagtcggca atgatgttgt gttaaatcgg
      721 gaaagcggct cgcaaaaaat gcacagattt gtaggcatga taagacgcgt agcgtcgcat
      781 cagacaccgg cgcacaaccg ccggatgcgg cgtgaacgcc ttatccggcc tacaaaatcg
      841 tctcttccgt caaaatccgt cgcgcgccta aaaaatggtc ctgccagaaa ggttcggcta
      901 accgacttac ccgaatggtt tccccggtgc gcggtgactc gataaactga ccgtctccaa
      961 gatatacgcc catatggtcg gctatctcac gactgtggat atggaaaaac agcaaatctc
     1021 cccggcgcag gtcgttattc gccacaatcg ttgcccgacg gtagtggtac atctcattgg
     1081 ccgtgcgcgg gagcttagcc tcaaggatct tgttgtaggc ataaaaaacc aacccgctac
     1141 agtcgaagcc tttatcaggc cgcgtgcccc cccagatata aggcttacct agctgctgct
     1201 ccaggcggtg aatcgccact tcggtgatgt tatgtagatg gtcgctgctt aagaaatgat
     1261 tattctccac caacgcctgc cagcgtctgt cactcgcctt gagcggcgca ggaaaccatt
     1321 ctggatgctg ttttatctgc tcccgattat gctggcgcag cgcccttctg cttttggcat
     1381 tgccttccac aatatagctg gcctgctttt tcagatgggt ttggtactgt tttaataaac
     1441 gcgcacggtt ttgcatccgc tggcgggcgg cgtagctaaa agagatatgt gatgtggtgg
     1501 cgctggcgag taaagggagc aaaacgcata acgaaaacac gcctggatgc agaaatctgc
     1561 cgtgcaagaa cgaagacatg aaggacataa accagctgaa tgaaggtgag aattttaaaa
     1621 gaccggagga gtaaacggga ttattaa
//
LOCUS       DADSYM010000279         1638 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2415
            SAMN08039579-rid11723173.denovo.281, whole genome shotgun sequence.
ACCESSION   DADSYM010000279 DADSYM010000000
VERSION     DADSYM010000279.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039579
            Sequence Read Archive: SRR6362533
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1638)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1638)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980162.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 03:39:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,484
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 4,347
            CDSs (with protein)               :: 4,347
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 43 of 85
            Pseudo Genes (incomplete)         :: 37 of 85
            Pseudo Genes (internal stop)      :: 19 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1638
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039579-rid11723173.denovo.281"
                     /strain="LGE2415"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8302 N 95.9628 W"
                     /collection_date="2015-08-24"
     gene            complement(<1..728)
                     /locus_tag="J1Y83_004325"
     CDS             complement(<1..728)
                     /locus_tag="J1Y83_004325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001011831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBA7632399.1"
                     /translation="MNFTFTDSGYTETNKNNPPIIFVHGFFMNNEMFKYQFDELKEKH
                     RVVCINVRGFDAPSEKIEPFSLYDIVDDILNVADSLQLDKFILGGMSMGGYISLRFAL
                     SHSERLSGLILMATQAEQDPPEIASSYMELCNGWQDPIIKENIIASLLPVFFGNNISE
                     AETWRQVWLGHKPENIRKAMNAMLERDDISERIQIITIPTLIIHGANDMGIPLDKAIQ
                     MHRMIAHSKLEVIPDANHALNITHA"
     gene            1326..1476
                     /locus_tag="J1Y83_004326"
                     /pseudo
     CDS             1326..1476
                     /locus_tag="J1Y83_004326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001689023.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
BASE COUNT          467 a          302 c          345 g          524 t
ORIGIN      
        1 gcatgagtga tatttagagc atgatttgcg tcgggaatga cttcaagctt agaatgcgca
       61 atcattctgt gcatttgaat tgctttatct aatggtatcc ccatatcgtt ggcaccatga
      121 ataatcaggg tggggattgt gattatctgg attctttctg aaatgtcgtc gcgctccagc
      181 attgcattca tggcctttct gatattttca ggtttatggc ctaaccatac ctgtctccat
      241 gtctctgctt cagagatatt atttccgaaa aaaacgggta ataatgaggc gattatattt
      301 tcctttatga ttgggtcttg ccatccatta cataactcca tgtaactgga cgcgatttct
      361 ggcggatcct gctctgcctg agttgccata aggattagtc ctgacaaacg ttctgaatgt
      421 gacagggcaa atcgtaatga tatatatccg cccatcgaca ttccccctaa aataaactta
      481 tccagttgta gtgagtctgc gacgtttaaa atgtcatcaa cgatgtcata gagcgagaat
      541 ggctcaattt tttcggaggg agcatcgaat ccccgaacat taatgcaaac aactctatgt
      601 ttttctttta gttcgtcaaa ctggtatttg aacatttcat tattcatgaa aaatccatga
      661 acgaatatga ttgggggatt gtttttattt gtttcagtgt atccagagtc agtaaacgta
      721 aaattcatga gtactccgtt atttagacag ttatgttata catttgacta atgatagaga
      781 agagctttat tgacctgctg aacagaaatc cacttgtcaa aaaccttctg aagtttatct
      841 accaagatgg acataatctt gtcaagtatt cgttaggata caaaagtatc ggtttaacag
      901 taaaatataa gaagtattcg tgcggctgta cgcatcattg atgtttcttt tctcacaaga
      961 catagctaat tctcggcacg ttgatgagct attttcatat attgtgcagt aaacattttt
     1021 cccggaggtg ccggcaagag caatacatcc taggttttgc tgattagtgt cgcttatgca
     1081 tcgatgataa gggatatttg ttcagtgagc ctgccgggaa tggtaccgcc agcatcgtca
     1141 gcttcagccc agtaaaaaga gacgtggaaa aatgcacaac aggcaccaca cgatatgcat
     1201 ggattcagat tgctcatgat tcaccaatac agctataaat cgtaaagaaa actccagtac
     1261 gttgtataca gaaacgtagc gtgattcact attgcacagg tccattacaa cgacaatatt
     1321 ttgtgatgtt tgcgtggtgc gccccacttc aggatgcggt agatctggag atcgcaacgg
     1381 agaaataaac atctatgctg gaaaaagtat cgggtgttgc tgaacagtgt atacacttcc
     1441 gttgcgccag atatcgagtg gtttattcaa ccataataaa cagtatgtat gtcataggtt
     1501 attaattgtg agttttttcg gtgcgttatt tgttggtttg atgttatgct tttgcgcccc
     1561 aaaaggttgt ttagctgtat tttatcaatt gattttcaat atcgtttagt aaagaaaaat
     1621 taagcaagca ggatgttg
//
LOCUS       DADSYM010000280         1633 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2415
            SAMN08039579-rid11723173.denovo.282, whole genome shotgun sequence.
ACCESSION   DADSYM010000280 DADSYM010000000
VERSION     DADSYM010000280.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039579
            Sequence Read Archive: SRR6362533
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1633)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1633)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980162.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 03:39:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,484
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 4,347
            CDSs (with protein)               :: 4,347
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 43 of 85
            Pseudo Genes (incomplete)         :: 37 of 85
            Pseudo Genes (internal stop)      :: 19 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1633
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039579-rid11723173.denovo.282"
                     /strain="LGE2415"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8302 N 95.9628 W"
                     /collection_date="2015-08-24"
     gene            complement(<1..558)
                     /locus_tag="J1Y83_004327"
     CDS             complement(<1..558)
                     /locus_tag="J1Y83_004327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308843.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA7632400.1"
                     /translation="MLSSCIGVVMKDGALLRSSSLFIAYMGCLGWGSAYFYGWGTSFY
                     YGFPWWIVGAGVDDVARSLFFAVIVIAIFLIGWGIGVVFFFAVKRKHSMQELNVFRLY
                     FAVELLFVPAIIEFSILRQKIQVPLLLLSAAIALAVTISIRSYGRFLSVSCFYDKPFI
                     KKHFFEIVMIAFVAYFWLFSFLTGYY"
     gene            complement(743..1432)
                     /locus_tag="J1Y83_004328"
     CDS             complement(743..1432)
                     /locus_tag="J1Y83_004328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HBA7632401.1"
                     /translation="MNTQYLQYVREQLMAATADLNGATKGQLEAWQEHAQFDTGTYKR
                     KKPRILDVVTGKMITLDNTPTSGKQSYAKGSSIALVSPVEFSTSSWRRAVLSLDEHQK
                     AWLLWCYSESVRWGHQVTITQWAWSEFKDLLSNRKIAGKTLDRLKTLIWLAAQDVKSE
                     LAGREAYEYQTLASLVGVTTKNWSETFTERWVAMKHIFLQLDSDALLLVTRTRSKQKA
                     AFLQQNIAKLD"
     gene            complement(1447..1569)
                     /locus_tag="J1Y83_004329"
     CDS             complement(1447..1569)
                     /locus_tag="J1Y83_004329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000971054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlcG family protein"
                     /protein_id="HBA7632402.1"
                     /translation="MMSESYISELLRCRWGLLCLCRFPDSVLNDYRMLKNYAKI"
BASE COUNT          521 a          407 c          281 g          424 t
ORIGIN      
        1 gtaatatcct gtcagaaatg aaaaaagcca aaaatatgcc acaaaagcaa tcatcacaat
       61 ctcaaaaaaa tgttttttta taaatggctt atcatagaag catgataccg ataaaaatcg
      121 cccataagat cttatcgaaa ttgtaaccgc cagcgcaatc gctgctgaca gtagcaaaag
      181 aggtacctga atcttctgtc tcaatataga aaactcaata attgccggca caaacaataa
      241 ttccacagca aaataaaggc gaaatacatt tagctcttgc atagaatgtt ttcttttcac
      301 tgcgaaaaag aatacaacac caatacccca accgataaga aatatagcaa tgacgataac
      361 tgcaaaaaat aaacttctgg caacatcatc aacacctgca cctacaatcc accatgggaa
      421 gccgtagtaa aaagaagtac cccatccata gaaataagca ctcccccatc caaggcatcc
      481 catgtaggca ataaaaagtg aagaactcct gagcagcgca ccatccttca taaccacccc
      541 aatacaagat gataacattg gcttacaact cataacaaaa gcaattcaat gccgtcaaga
      601 ggttacaggc taaaaaaact ctattacata gcagccagca tgtttaccgt acaagtacaa
      661 ctcagggcat aaaaaaaacc cactcggcag cgggtttata cattttttac aacataccaa
      721 atttgcatga agtatatggc ttttaatcca gttttgcaat attttgctgt aaaaatgctg
      781 ccttttgttt tgaacgtgtt ctcgtcacaa gcaataaagc atcactatca agctgtagaa
      841 aaatgtgctt cattgcaacc cagcgttcag taaatgtctc ggaccagttt tttgttgtca
      901 ctcccaccaa tgatgccagt gtctggtatt cataggcctc acgccctgca agttcgctct
      961 tcacatcctg tgcagccagc cagattaacg tcttcaggcg atccagtgtc ttacctgcaa
     1021 tttttctgtt acttaacaaa tctttaaact cgctccatgc ccattgcgtt atggtgacct
     1081 gatgccccca tcgaacgctt tcgctgtaac accaaagcaa ccatgctttc tgatgttcat
     1141 cgagagacaa aaccgcgcgg cgccatgaag aggttgagaa ttcaaccggg ctgaccaaag
     1201 caatggatga accttttgcg tacgactgct taccggaagt cggcgtatta tccagcgtaa
     1261 tcatcttgcc agttaccaca tccagaatgc gcggcttctt tcgtttgtat gtaccagtat
     1321 caaattgtgc atgctcctgc caggcttcga gctggccttt cgttgctccg ttcaagtcag
     1381 cagtagctgc cataagttgc tcacgaacat actgtaaata ttgggtattc atgcagtaaa
     1441 tcctttctat attttggcat aattcttcaa cattcggtaa tcgttcaaaa ccgaatcggg
     1501 gaaacgacat aagcacagga gcccccagcg acagcgaagg agttctgata tataagactc
     1561 agacatcatt cattccccgg ttctccaata tctgtttcac tcatcatcca taactacctg
     1621 taattgcccc cct
//
LOCUS       DADSYM010000281         1625 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2415
            SAMN08039579-rid11723173.denovo.283, whole genome shotgun sequence.
ACCESSION   DADSYM010000281 DADSYM010000000
VERSION     DADSYM010000281.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039579
            Sequence Read Archive: SRR6362533
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1625)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1625)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980162.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 03:39:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,484
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 4,347
            CDSs (with protein)               :: 4,347
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 43 of 85
            Pseudo Genes (incomplete)         :: 37 of 85
            Pseudo Genes (internal stop)      :: 19 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1625
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039579-rid11723173.denovo.283"
                     /strain="LGE2415"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8302 N 95.9628 W"
                     /collection_date="2015-08-24"
     gene            <1..>1625
                     /gene="tir"
                     /locus_tag="J1Y83_004330"
     CDS             <1..>1625
                     /gene="tir"
                     /locus_tag="J1Y83_004330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001339882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type III secretion system LEE translocated
                     intimin receptor Tir"
                     /protein_id="HBA7632403.1"
                     /translation="GNLGNNVNGNHLIPPAPPLPSQTDGAARGGTGHLISSTGALGSR
                     SLFSPLRNSVADSVDSRDIPGLPANPSRLAAATSETCLLGGFEVLHDKGPLDILNTQI
                     GPSAFRVEAQADGTHAAIGEKNGLEVSVTLSPQELSSLQAIDTEGKNRFVFTGGRGGS
                     GHPMVTVASDIAEARAKILAKLDPDNQGGRQAKDVDTRSVGVGSASGMDDGVVSETHT
                     STTTSSVRSDPKFWVSVGAIAAGLAGLAATGIAQALALTPEPDDPTTTDPDQAANAAE
                     SATKDQLTQEAFKDPENQKVNIDENGNAFPSGELKDDIVAQIAEQAKEAGEVARQQAV
                     ESNAQAQQRYDDQHARRQEELQLSSGIGYGLSSALIAAGGIGAGVTTALHRRNQPTEQ
                     TTTTTHTVVQQQTGGNTPAQGGTDATRAEDASLNRRDSQGSVASTHWSDSSSEVVNPY
                     AEVGGARNSLSAHQPEEHIYDEVAADPGYSVIQNFSGSGPVTGRLIGTPGQGIQSTYA
                     LLANSGGLRLGMGGLTSGGESAVSSVNAAPTPG"
BASE COUNT          438 a          355 c          451 g          381 t
ORIGIN      
        1 ttggtaacct tggtaataat gtaaatggca atcatttaat tccccctgcg ccgccactac
       61 cttcacaaac agacggcgcg gcacggggag gaactggtca tctaattagc tctacaggag
      121 cattaggatc tcgttcattg ttttctcccc tgagaaattc tgtggctgat tctgtcgatt
      181 ccagagatat tccaggactt cctgcaaacc catcgaggct tgctgcagct acatctgaga
      241 catgcttgct tggaggattt gaagttctcc atgataaggg gccacttgat attctcaata
      301 cgcaaattgg accctctgca tttcgtgttg aagcgcaggc agatggtact catgccgcta
      361 ttggagaaaa aaatggtttg gaggttagcg ttacattaag tcctcaagaa ttgagcagct
      421 tgcaagctat tgatactgag ggtaaaaaca gatttgtttt taccggggga cgtggcggta
      481 gtgggcatcc gatggtcact gtcgcatcag atatcgcgga agctcgtgcg aaaatactgg
      541 ccaaattaga cccagacaat cagggagggc gtcaagccaa ggacgttgat acgcgttctg
      601 ttggtgttgg aagcgcttcg ggaatggatg atggcgttgt tagcgaaacc catacttcaa
      661 caacaacttc cagcgttcgt tcagatccta aattctgggt ttctgtcggc gcaattgctg
      721 ctggcttagc ggggctggcg gcaactggta ttgcacaggc gttggctttg acaccggaac
      781 cggatgatcc tacaaccacc gatcctgatc aggccgcaaa tgctgcagaa agtgcaacaa
      841 aagatcagtt aacgcaagaa gcattcaagg accctgagaa ccagaaagtt aacatcgatg
      901 agaacggaaa tgcctttccg tctggggaat taaaagatga tattgttgcg caaatagcag
      961 aacaagctaa agaggctggt gaggtggcca gacagcaggc tgttgaaagc aatgcacagg
     1021 cgcagcagcg atatgatgat cagcatgcca gacgtcagga ggaattacag ctttcgtcgg
     1081 gtattggtta cggcctcagc agtgcattga ttgctgctgg gggaattggt gctggtgtaa
     1141 cgactgcgct ccatagacga aatcagccaa cagaacagac gactacaaca acacatacgg
     1201 ttgtgcagca acagaccgga gggaataccc cagcacaagg tggcactgat gccacaagag
     1261 cagaagacgc ttctctgaat agacgtgatt cgcaggggag tgttgcatcg acacactggt
     1321 cagattcctc tagcgaagtg gttaatccat atgctgaagt tgggggggct cggaatagtc
     1381 tatcggctca tcagccagaa gagcatattt atgatgaggt cgctgcagat cctggttata
     1441 gcgttattca gaatttttca gggagcggcc cagttaccgg aaggttaata ggaactccag
     1501 ggcaaggtat ccaaagtact tatgcgcttc tggcaaacag cggcggattg cgtttaggta
     1561 tgggaggatt aacgagtggt ggcgagagcg cagtaagttc tgtaaatgcc gcaccaacgc
     1621 cggga
//
LOCUS       DADSYM010000282         1588 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2415
            SAMN08039579-rid11723173.denovo.284, whole genome shotgun sequence.
ACCESSION   DADSYM010000282 DADSYM010000000
VERSION     DADSYM010000282.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039579
            Sequence Read Archive: SRR6362533
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1588)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1588)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980162.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 03:39:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,484
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 4,347
            CDSs (with protein)               :: 4,347
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 43 of 85
            Pseudo Genes (incomplete)         :: 37 of 85
            Pseudo Genes (internal stop)      :: 19 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1588
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039579-rid11723173.denovo.284"
                     /strain="LGE2415"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8302 N 95.9628 W"
                     /collection_date="2015-08-24"
     gene            <1..633
                     /locus_tag="J1Y83_004331"
     CDS             <1..633
                     /locus_tag="J1Y83_004331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001557812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HBA7632404.1"
                     /translation="QREPEENLSQGRKFLLGGHKVVEIAALNSCVLQQVKDSFLGMGF
                     VGEQQLSDVAKSMGWMNDSGGDILKKKGVIRIAMLHHHLTSINEVEDAYLDSRYSVTL
                     DAERLLRWVVRHKVDYILHGHMHRSSFITITKKLSPLEPVTDSNPEHTFQIVSLGSSG
                     VVSSELPSQDCANYACVLDFSGEKLTFNFFKLDKQSGEKVAATYIVEGLL"
     gene            630..1466
                     /locus_tag="J1Y83_004332"
     CDS             630..1466
                     /locus_tag="J1Y83_004332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001557813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HBA7632405.1"
                     /translation="MKLLITDLDNTLYDWVSFYSQSFSAMAEQLSKDIDVPLDTLLSE
                     YKTIHQKFGNSEKPFATLELPSVISYFGTNDKTILQEKLTNVFQAFSTKRNQTLMLYP
                     TVKETLELLKKRGVKIVGHTEALEFNSLYRLNKLGIIDFFDHLYTLEDIHNIHPNPKN
                     AKKIPVKDDFVIRLSCAESKPNPKLLEHICSFEGVSVEDAVYIGDSITKDMSMAKAVG
                     VTAVWANYGRQFSPELWKVLVKITHWKDADVEREEQLRKAFSNVRPDYVVNSFSELLN
                     LK"
BASE COUNT          505 a          228 c          339 g          516 t
ORIGIN      
        1 caaagggaac ctgaagaaaa tttatcacaa ggaaggaaat ttttactagg tggccataaa
       61 gttgttgaaa tagccgcatt gaactcatgc gtattacaac aagtaaaaga ttcatttttg
      121 ggcatgggat ttgtcggtga gcaacaactc agtgatgttg caaaatctat ggggtggatg
      181 aatgatagcg gaggagatat actcaaaaag aaaggtgtta taagaattgc tatgcttcac
      241 catcacctaa cttctattaa tgaagttgaa gatgcctatc ttgattcaag atatagtgta
      301 acattagatg cagagcgatt attacgttgg gtagtaaggc ataaagttga ttatattctg
      361 catggtcata tgcacagaag tagttttatt acaataacaa aaaaactatc tccgttagaa
      421 ccagtgacgg attcaaatcc tgaacataca ttccaaattg tttctttggg aagttcagga
      481 gttgttagtt cggaattgcc aagtcaggat tgtgctaact atgcatgcgt actggatttt
      541 tctggcgaaa agctaacttt taattttttc aagcttgata aacagagcgg agaaaaagtg
      601 gcggctactt atattgttga aggtttatta tgaaattact tattactgat ttggataata
      661 cactgtacga ttgggtgagt ttttactccc agtcatttag tgccatggca gagcaattat
      721 ctaaggatat tgatgtgcct ttggatacgc tcctgtcaga atataaaaca attcatcaga
      781 agtttggtaa ctcagaaaaa ccatttgcta ctttagagct accatctgtg atatcttact
      841 ttggaactaa tgataaaacg attttgcagg aaaaacttac taatgttttt caggcgttta
      901 gcacaaaacg taaccagaca ttaatgttgt atcctactgt aaaagaaacg ctggaattat
      961 tgaagaaacg gggggtaaaa attgtagggc atactgaagc tttggagttt aattctttat
     1021 atcgtctgaa taagcttggt ataattgatt tttttgatca tctttataca ttagaagata
     1081 ttcataatat ccacccgaat cccaaaaatg caaaaaaaat acctgtgaag gatgactttg
     1141 taattagatt gtcctgcgct gagtcaaagc caaaccctaa attgcttgag cacatatgtt
     1201 cttttgaagg agttagtgtt gaagatgcgg tatacattgg cgattctata actaaagata
     1261 tgtccatggc taaagctgtg ggggttactg ctgtttgggc taattatggg cgacagtttt
     1321 cacctgaatt gtggaaagtt ttagtgaaaa tcacacactg gaaagatgca gatgtggaga
     1381 gggaggaaca acttagaaaa gcattctcca atgttagacc tgattatgtt gttaatagtt
     1441 tttcagagct gctgaatttg aaatgagcat gtgcaggctc caaattttta atgatttgga
     1501 gttctttatg gggttatttt ttaatttggt gatttttccc aatattgaga tgttgttgaa
     1561 ctcgtgagtt atatttaacg tatttgat
//
LOCUS       DADSYM010000283         1588 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2415
            SAMN08039579-rid11723173.denovo.285, whole genome shotgun sequence.
ACCESSION   DADSYM010000283 DADSYM010000000
VERSION     DADSYM010000283.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039579
            Sequence Read Archive: SRR6362533
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1588)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1588)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980162.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 03:39:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,484
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 4,347
            CDSs (with protein)               :: 4,347
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 43 of 85
            Pseudo Genes (incomplete)         :: 37 of 85
            Pseudo Genes (internal stop)      :: 19 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1588
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039579-rid11723173.denovo.285"
                     /strain="LGE2415"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8302 N 95.9628 W"
                     /collection_date="2015-08-24"
     gene            complement(30..875)
                     /gene="nhoA"
                     /locus_tag="J1Y83_004333"
     CDS             complement(30..875)
                     /gene="nhoA"
                     /locus_tag="J1Y83_004333"
                     /EC_number="2.3.1.118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-hydroxyarylamine O-acetyltransferase"
                     /protein_id="HBA7632406.1"
                     /translation="MTPILNHYFARINWLGAAAANIDTLRALHLKHNCTIPFENLDVL
                     LPREIQLDDQSLEDKLVIARRGGYCFEQNGVFERVLRELGFNVRSLLGRVVLSNPPAL
                     PPRTHRLLLVELEGEKWIADVGFGGQTLTAPIRLMPDIMQTTPHGEYRLLQEGDDWVL
                     QFNHHQHWQSMYRFDLCEQQQSDYVMGNFWSAHWPQSHFRHHLLMCRHLPDGGKLTLT
                     NFHFTHYENGHAVEQRNLPDVASLYAVMQEQFGLGVDDAKHGFTVDELALVMAAFDTH
                     PEAGK"
     gene            1050..>1588
                     /locus_tag="J1Y83_004334"
     CDS             1050..>1588
                     /locus_tag="J1Y83_004334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415979.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HBA7632407.1"
                     /translation="MSQFIPVELHHASRLLNHGPTVMITSFDEQSQRRNIMAAAWSMP
                     VEFEPPRVAIVVDKSTWTRELIERNGKFGIVIPGVAATNWTWAVGSVSGREEDKFNCY
                     GIPVVRGPVLGLPLVEEKCLAWMECRLLPATSAQEKYDTLFGEVVSAAADARVFVEGR
                     WQFDDDKLNTLHHLGAGTFV"
BASE COUNT          403 a          419 c          383 g          383 t
ORIGIN      
        1 ttacgccaca tccggcaacc tgacataaat tattttcccg cctccgggtg cgtatcaaac
       61 gccgccatca ccagcgctaa ctcatccaca gtaaagccat gtttcgcatc atctacgccc
      121 agaccaaact gttcctgcat cacggcatat aacgacgcca catctggtag atttcgctgc
      181 tcaactgcgt gcccattttc ataatgggta aaatgaaaat tggtcagcgt tagcttgcca
      241 ccgtccggca aatggcgaca catcagcaaa tgatggcgaa aatgcgactg cggccagtgc
      301 gccgaccaga aattgcccat cacataatcg ctttgttgct gctcgcagag atcaaaacgg
      361 tacatcgact gccagtgctg atgatgatta aactgcaata cccaatcatc accctcttgc
      421 aacaaccgat actctccgtg tggcgtggtt tgcatgatat ccggcattaa acgaatcggc
      481 gcggttagcg tctgcccacc gaaaccgaca tcagcaatcc atttttcccc ttccagttcc
      541 accaacagca aacgatgggt gcgcggcggt aatgctggcg gatttgataa cactacgcga
      601 cctaacaagc tgcgaacgtt aaaccccagc tcgcgtaaca cccgctcaaa cacgccattc
      661 tgctcaaaac agtaaccgcc acgacgggct atcaccagtt tatcttccag cgattgatca
      721 tcaagctgta tttccctcgg cagcaaaacg tcgagatttt caaacggaat ggtgcaattg
      781 tgtttcaggt gcaatgcacg cagcgtatca atattggccg cagcagctcc caaccagtta
      841 atacgggcaa aataatgatt cagaatgggc gtcatcaggc gtttccctta gtggttttct
      901 caacttataa cctggaatag acgccgcact ggctattttg tgcgatcgcg gacatgcatc
      961 acgttcgcaa atcgccaatc ccgttaattt cgttacttat tgcgttacga gctacgcttt
     1021 taaaacaccc ttcatagcga ggcgatatcg tgagccaatt cattcccgtc gaattacacc
     1081 atgccagccg tctgttaaat catggcccaa ctgtcatgat caccagtttc gatgagcaat
     1141 cccagcggcg taacattatg gcagcagcct ggtcgatgcc cgtagagttt gaaccgccac
     1201 gcgtggcgat tgtggtagat aaatcgacat ggaccaggga gttgattgaa cgtaacggta
     1261 agtttggcat cgttatcccg ggcgttgcgg cgactaactg gacgtgggcg gtgggaagtg
     1321 tgtcgggtcg tgaagaagat aaatttaatt gctacggcat tccggtagtg agaggcccgg
     1381 tgcttggttt gccgctagtc gaggaaaaat gtctggcgtg gatggagtgt cgcctgctac
     1441 ctgcaacttc cgcgcaagaa aaatacgata cgctatttgg cgaagtggta tcagcagcgg
     1501 cagacgcacg ggtatttgtc gaaggccgct ggcagtttga tgatgataag ctcaatacgt
     1561 tgcatcattt aggtgctggc acgtttgt
//
LOCUS       DADSYM010000284         1537 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2415
            SAMN08039579-rid11723173.denovo.286, whole genome shotgun sequence.
ACCESSION   DADSYM010000284 DADSYM010000000
VERSION     DADSYM010000284.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039579
            Sequence Read Archive: SRR6362533
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1537)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1537)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980162.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 03:39:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,484
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 4,347
            CDSs (with protein)               :: 4,347
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 43 of 85
            Pseudo Genes (incomplete)         :: 37 of 85
            Pseudo Genes (internal stop)      :: 19 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1537
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039579-rid11723173.denovo.286"
                     /strain="LGE2415"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8302 N 95.9628 W"
                     /collection_date="2015-08-24"
     gene            <1..556
                     /gene="escR"
                     /locus_tag="J1Y83_004335"
     CDS             <1..556
                     /gene="escR"
                     /locus_tag="J1Y83_004335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312610.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type III secretion system LEE export apparatus
                     protein EscR"
                     /protein_id="HBA7632408.1"
                     /translation="FLKISIVLGILKNALGIQQVPPNMALTSVSLILTMFIMSPIILQ
                     INDNISQEPINYTDSDFFQKVDEKILSPYRGFLEKNTEKDNVEFFERAAQKKLGNETI
                     LKKDSLFILLPAFTMGQLEAAFKIGFLLYLPFIAIDLIISNILLALGMMMVSPVTISI
                     PFKILLFILVGGWQKLFEFLLVVN"
     gene            556..825
                     /gene="escS"
                     /locus_tag="J1Y83_004336"
     CDS             556..825
                     /gene="escS"
                     /locus_tag="J1Y83_004336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE export apparatus
                     protein EscS"
                     /protein_id="HBA7632409.1"
                     /translation="MDTGYFVQLCVQTFWIIFILSLPTVIAASVIGIIISLVQAITQL
                     QDQTLPFLLKIIAVFATLALTYHWMGTTIINFSSIIFEMIPKVNG"
     gene            825..>1537
                     /gene="escT"
                     /locus_tag="J1Y83_004337"
     CDS             825..>1537
                     /gene="escT"
                     /locus_tag="J1Y83_004337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE export apparatus
                     protein EscT"
                     /protein_id="HBA7632410.1"
                     /translation="MNEIMTVIVSSFYCILRPLGMFIILPIFSTGVLLSNFIRNSIMI
                     AFTLPIIVENYTFSEKLPSGIFQLTGIALKEISIGFFIGLSFTILFWAIDAAGQIIDT
                     LRGSTISSIFNPSISDSSSITGVILYQFISVIFVIHGGIQSILDKLYLSYEILPLQAD
                     IAFNRALIDFLFSLWDSFIKLMLSFSVPMIIGIFLCDMGFGFLNKTAPQLNVFTLSLP
                     VKSLIAIFILLLVIHVFPD"
BASE COUNT          445 a          259 c          272 g          561 t
ORIGIN      
        1 attcctgaaa atttctattg tactggggat attgaagaac gcattaggca ttcaacaggt
       61 accaccaaac atggcgctaa catcagtgtc tttgatactg acaatgttta ttatgtctcc
      121 gataatatta cagataaatg ataatatttc tcaggaacca atcaattata ccgactctga
      181 tttttttcaa aaagttgatg agaaaatatt atcaccatat cgcggttttt tagaaaaaaa
      241 tacagagaaa gacaatgtag agttttttga acgtgcagct caaaaaaaat tgggtaatga
      301 aactatctta aaaaaagact ctctatttat actgttaccg gctttcacga tggggcagct
      361 tgaagctgca ttcaagatag gttttttgct ctatttaccc tttattgcga tagatttgat
      421 catttccaat atcttattgg ccttgggtat gatgatggta tcgccagtaa caatttcgat
      481 tccttttaag atcttgttat tcattcttgt tggaggctgg caaaaattat tcgaatttct
      541 gttggtggtg aattgatgga tactggatat tttgttcaat tatgtgtgca aacgttctgg
      601 ataatattta tcctctcatt gcctacagtc atagcggcct ctgttatcgg tattattatt
      661 agtctggtcc aggctataac gcagttacag gatcaaacat tgcctttttt gctaaaaata
      721 atagcagtgt ttgctacgct tgccctgact tatcactgga tgggaacaac aatcatcaac
      781 ttctcgtcga taatatttga aatgattccg aaggtgaacg gctaatgaat gagataatga
      841 cggtcatagt atcatcattt tattgtatac tcaggccgct ggggatgttt attatcctac
      901 caatattttc aacaggtgtt ttactcagta actttattcg caactcaatt atgattgcgt
      961 ttacgcttcc gatcatagta gagaactata cattttcgga aaaattgcca tctggaatct
     1021 ttcagttgac aggaatagcg ctaaaagaaa taagtattgg ttttttcatt gggttatcat
     1081 ttactattct tttttgggca atagatgcgg ctgggcagat tattgatact ctaagaggtt
     1141 caacaatatc ttcaattttt aacccgtcca taagtgattc atcttctatc actggcgtta
     1201 ttttgtacca atttatctct gtgatctttg ttattcatgg tgggatacaa agcatcctag
     1261 ataagctata tttatcctac gagatattac cattacaagc cgatattgca ttcaatcgtg
     1321 ctttaataga ttttttgttt tctctatggg attcatttat taaactgatg ttatcatttt
     1381 cagttcccat gattatcggt atattcttat gtgatatggg gtttgggttt cttaacaaaa
     1441 cagcacctca gctaaacgta ttcacattat cactgccagt aaaaagtttg atagcaatct
     1501 ttatattgct attagttatt catgtttttc ctgattt
//
LOCUS       DADSYM010000285         1526 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2415
            SAMN08039579-rid11723173.denovo.287, whole genome shotgun sequence.
ACCESSION   DADSYM010000285 DADSYM010000000
VERSION     DADSYM010000285.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039579
            Sequence Read Archive: SRR6362533
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1526)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1526)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980162.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 03:39:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,484
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 4,347
            CDSs (with protein)               :: 4,347
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 43 of 85
            Pseudo Genes (incomplete)         :: 37 of 85
            Pseudo Genes (internal stop)      :: 19 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1526
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039579-rid11723173.denovo.287"
                     /strain="LGE2415"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8302 N 95.9628 W"
                     /collection_date="2015-08-24"
     gene            171..>1526
                     /locus_tag="J1Y83_004338"
     CDS             171..>1526
                     /locus_tag="J1Y83_004338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HBA7632411.1"
                     /translation="MQKTLMASLIGLAVCTGNAFSPALAAEAKQPNLVIIMADDLGYG
                     DLATYGHQIVKTPNIDRLAQEGVKFTDYYAPAPLSSPSRAGLLTGRMPFRTGIRSWIP
                     SGKDVALGRNELTIANLLKAQGYDTAMMGKLHLNAGSDRTDQPQAQDMGFDYSLVNTA
                     GFVTDATLDNAKERPRYGMVYPTGWLRNGQPTPRADKMSGEYVSSEVVNWLDNKKDSK
                     PFFLYVAFTEVHSPLASPKKYLDMYSQYMSAYQKQHPDLFYGDWADKPWRGVGEYYAN
                     ISYLDAQVGKVLDKIKAVGEEDNTIVIFTSDNGPVTREARKVYELNLAGETDGLRGRK
                     DNLWEGGIRVPAIIKYGKHLPQGMVSDTPVYGLDWMPTLAKMMNFKLPTDRTFDGESL
                     VPVLEQKALKREKPLIFGIDMPFQDDPTDEWAIRDGDWKMIIDRNNKPKYLYNLKSDR
                     YE"
BASE COUNT          425 a          321 c          378 g          402 t
ORIGIN      
        1 ggccggaacg ggttaaatgg cggtttaact attgcctgga agttctatcc tcgctggaaa
       61 gccagtgtga cgtggcgtta tttcgataat aagctgggct acgatggctt tggcgatcaa
      121 atgatttata tgcttggtta tgatttctaa ttttttatca gggaataatt atgcagaaaa
      181 cgttaatggc cagtttgatc ggccttgcag tttgcacagg gaatgctttt agtcctgcct
      241 tagccgcaga ggctaaacaa cctaatttag tcattattat ggcggatgat ttaggttatg
      301 gcgatttagc aacatatggt catcagatcg ttaaaacacc taatatcgac aggcttgccc
      361 aggaaggggt caaatttact gactactatg cccccgctcc tttaagttca ccttcacgcg
      421 cagggctatt aaccggccgg atgccatttc gtactggaat tcgctcatgg attccttcag
      481 gcaaagatgt tgccttaggg cgtaacgaac tcacgattgc taatctactc aaagcgcaag
      541 ggtacgacac ggcaatgatg ggtaagctgc atctgaatgc aggcagcgat cgcaccgatc
      601 agccacaagc acaagatatg ggctttgatt actcactggt taatacggcg ggctttgtta
      661 ccgacgccac gctggataac gctaaagaac gcccgcgtta tggcatggtt tacccgacag
      721 gctggctacg taacgggcaa cccactccac gagctgataa aatgagcggt gagtatgtca
      781 gttcggaagt cgtcaactgg ctggataaca aaaaggacag caagcctttc ttcctctatg
      841 ttgcttttac cgaagtgcat agccccctgg cttcgcccaa aaaatacctc gatatgtact
      901 cacaatatat gagcgcgtat cagaagcagc atcctgattt attttatggc gactgggcag
      961 acaaaccctg gcgtggtgtg ggggaatatt atgccaatat cagctatctg gatgcacagg
     1021 ttggaaaagt gctggataaa atcaaagctg tgggtgaaga agataacaca atcgttattt
     1081 ttaccagtga taacggtccg gtaacgcgtg aagcgcgcaa agtgtatgag ctgaatttgg
     1141 caggggaaac ggatggatta cgcggtcgca aggataacct ttgggaaggc ggaattcgtg
     1201 ttccagccat tattaaatat ggtaaacatc taccacaggg aatggtttca gatacacccg
     1261 tttatggtct ggactggatg cctactttag cgaaaatgat gaacttcaaa ttacctacag
     1321 accgtacttt cgatggtgaa tcgctggttc ctgttcttga gcaaaaagca ttgaaacgcg
     1381 aaaagccatt aattttcggg attgatatgc cattccagga tgatccaacc gatgaatggg
     1441 cgatccgtga tggtgactgg aagatgatta tcgatcgcaa taataaaccg aaatatctct
     1501 acaatctgaa atctgatcgt tatgaa
//
LOCUS       DADSYM010000286         1511 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2415
            SAMN08039579-rid11723173.denovo.288, whole genome shotgun sequence.
ACCESSION   DADSYM010000286 DADSYM010000000
VERSION     DADSYM010000286.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039579
            Sequence Read Archive: SRR6362533
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1511)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1511)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980162.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 03:39:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,484
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 4,347
            CDSs (with protein)               :: 4,347
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 43 of 85
            Pseudo Genes (incomplete)         :: 37 of 85
            Pseudo Genes (internal stop)      :: 19 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1511
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039579-rid11723173.denovo.288"
                     /strain="LGE2415"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8302 N 95.9628 W"
                     /collection_date="2015-08-24"
     gene            19..>1511
                     /locus_tag="J1Y83_004339"
     CDS             19..>1511
                     /locus_tag="J1Y83_004339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host specificity protein J"
                     /protein_id="HBA7632412.1"
                     /translation="MGKGSSKGHTPREAKDNLKSTQLLSVIDAISEGPIEGPVDGLKS
                     VLLNSTPVLDSEGNTNISGVTVVFRAGEQEQTPPEGFESSGSETVLGTEVKYDTPIIR
                     TITSANIDRLRFTFGVQALVETTSKGDRNPSEVRLLVQIQRNGGWVTEKDITIKGKTT
                     SQYLASVVVDNLPPRPFNIRMRRMTPDSTTDQLQNKTLWSSYTEIIDVKQCYPNTALV
                     GVQVDSEQFGSQQVSRNYHLRGRILQVPSNYNPQTRQYSGIWDGTFKPAYSNNMAWCL
                     WDMLTHPRYGMGKRLGAADVDKWALYVIGQNCDQSVPDGSGGTEPRITCNAYLTTQRK
                     AWDVLSDFCSAMRCMPVWNGQTLTFVQDRPSDKVWTYNRSNVVMPDDGAPFRYSFSAL
                     KDRHNAVEVNWIDPNNGWETATELVEDTQAIARYGRNVTKMDAFGCTSRGQAHRAGLW
                     LIKTELLETQTVDFSVGAEGLRHVPGDVIEICDDDYAGISTGGRVLAV"
BASE COUNT          353 a          379 c          484 g          295 t
ORIGIN      
        1 gtttatggag cgtgacgaat gggtaaaggc agcagtaagg ggcatacccc gcgtgaagcg
       61 aaggacaacc tgaaatcaac gcagttgctg agtgtgattg atgccattag tgaaggcccg
      121 attgaaggtc cggtggatgg gttaaaaagc gtgctgctga acagtacgcc ggtgctggac
      181 agtgagggga ataccaatat atccggcgtc acggtggtgt tccgggcagg tgagcaggag
      241 cagacaccgc ctgaggggtt tgaatcctcc ggctccgaga cggtgctggg tacggaagtg
      301 aaatacgaca cgccgatcat ccggactatc acgtctgcaa acatcgaccg tctgcgtttt
      361 accttcggtg tgcaggctct ggtggaaacc acctcaaaag gggaccggaa tccatcggaa
      421 gtccgcctgc tggttcagat acaacgtaac ggtggctggg tgacggaaaa agacatcacc
      481 attaagggca aaaccacctc gcagtatctg gcatcggtgg tggtggataa cctgccgccg
      541 cgcccgttca atatccggat gcgcaggatg acaccggaca gcaccacaga ccagctgcag
      601 aacaaaacgc tctggtcgtc atacaccgaa atcatcgatg tgaaacagtg ctacccgaac
      661 acggcactgg ttggcgtaca ggtggactca gagcagttcg gcagtcagca ggtgagccgt
      721 aattatcatc ttcgcgggcg cattctgcag gtgccgtcga actataaccc gcagacgcgg
      781 caatacagcg gtatatggga cggaacgttt aagccagcat acagcaacaa catggcctgg
      841 tgtctgtggg atatgctgac ccatccgcgc tacggcatgg ggaaacgtct tggtgcagca
      901 gatgtggata aatgggcgct gtatgtcatc ggccagaatt gcgaccagtc ggtgccggac
      961 ggctctggcg gcacggagcc gcgcatcacc tgtaacgctt acctgaccac acagcgcaag
     1021 gcgtgggatg tgctcagtga tttctgctcg gcgatgcgct gtatgccagt atggaacggg
     1081 cagacgttga cgttcgtgca ggaccgaccg tcggataagg tgtggaccta taaccgcagt
     1141 aatgtggtga tgccggatga tggcgcgccg ttccgctaca gcttcagcgc cctgaaggac
     1201 cgccataatg ccgttgaggt gaactggatt gacccgaaca acggttggga gacagcgaca
     1261 gagcttgtgg aggacacaca ggccattgcc cgttacggtc gtaacgtcac gaagatggat
     1321 gcctttggct gtaccagccg tgggcaggcg caccgcgccg ggctgtggct gattaaaacg
     1381 gaactgctgg aaacgcagac tgtggatttc agcgtggggg ccgaaggcct tcgccatgtg
     1441 ccgggcgatg ttattgaaat ctgcgatgat gactatgcgg ggatcagcac cggtggtcgt
     1501 gtgctggcgg t
//
LOCUS       DADSYM010000287         1495 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2415
            SAMN08039579-rid11723173.denovo.289, whole genome shotgun sequence.
ACCESSION   DADSYM010000287 DADSYM010000000
VERSION     DADSYM010000287.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039579
            Sequence Read Archive: SRR6362533
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1495)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1495)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980162.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 03:39:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,484
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 4,347
            CDSs (with protein)               :: 4,347
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 43 of 85
            Pseudo Genes (incomplete)         :: 37 of 85
            Pseudo Genes (internal stop)      :: 19 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1495
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039579-rid11723173.denovo.289"
                     /strain="LGE2415"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8302 N 95.9628 W"
                     /collection_date="2015-08-24"
     gene            194..1453
                     /locus_tag="J1Y83_004340"
     CDS             194..1453
                     /locus_tag="J1Y83_004340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001356890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytoplasmic protein"
                     /protein_id="HBA7632413.1"
                     /translation="MFTNVNVDCCKTPGCKNLGLLNSQDYVAQGKNILCRECGYLFPV
                     ISEQSLNIYRNIVNHSWRGLICQCSTCGGTSLKKYGYSAQGQRRMYCHHCEKTFITLE
                     HVITTPRGAQLALMIEQGEALADIRKSLLLNSTGLSRELLKLAREANYKESRQCFPAS
                     DITLSTRAFRVKYNGSNNSLYALVTAEEQSGRVVAISTNYSPSAVEQHYQYTSNYEER
                     MSPGTLAHHVQRKELLTMRRDTLFDIDYGPAVLHQNDPGMLVKPVLPAYRHFELVRIL
                     TDEHSNNVQHYLDHECFILGGCLMANLQHIHQGRCHISFVKERGVAPATIDFPPRLFL
                     SGGVRNNVWRAFSNRNYSMAVCNLTGSKKVREMRHATLNSATRFIHFVENHPFLISLN
                     RMSPANVVSTLDILKHLWNKKLEHGTI"
BASE COUNT          428 a          315 c          324 g          428 t
ORIGIN      
        1 atattgataa tgattctcta atgaaagcca gaggtgataa accagaagcg gtgacctggg
       61 gctaacctga taatcataat aaatgtaatg tatgccattt agggctatgc ccgcctgatt
      121 cagtgctacg cattgaatca ggttcttttc gcgttgaaca atatcaagat acggctattc
      181 gcagggagtg gatatgttta ccaacgtaaa tgttgattgt tgcaaaacac caggatgtaa
      241 aaacctgggg ttgctgaata gccaggatta tgtcgcacag ggtaaaaata ttttatgccg
      301 tgaatgtggt tacttgtttc cagtgatatc tgaacagtcg cttaatattt atcgtaatat
      361 tgtgaatcac tcctggagag gtttgatttg ccaatgttca acttgcggag gcacgtccct
      421 caaaaaatat ggatattctg cacaaggcca gagaagaatg tattgccatc attgtgagaa
      481 aacatttatc actctggaac atgtaattac cacaccacga ggagcccagt tagcattgat
      541 gattgagcaa ggggaggcac ttgcggatat cagaaagtca ttacttctta acagcactgg
      601 acttagccgt gaactgttaa aattagcgcg tgaagcaaac tataaagaaa gtcgacagtg
      661 tttcccagct tctgatatta ccctgagtac ccgcgctttt cgcgtcaagt ataatggtag
      721 caataactct ctttatgctc ttgttaccgc agaagaacaa agcggcaggg tggttgccat
      781 ctcaaccaat tactccccat ctgccgtaga gcaacattat caatacacat cgaactatga
      841 agagcgtatg tctccaggga cgctggcaca tcatgtccag cgcaaagagt tacttactat
      901 gcggcgggat accttgtttg atattgatta cggcccggca gttttacatc aaaacgatcc
      961 gggaatgttg gtaaaaccgg ttcttccggc atatcgtcat tttgaactgg tcagaatact
     1021 gaccgatgag cattccaaca acgttcagca ttaccttgat cacgaatgct ttatattggg
     1081 cggctgcctg atggctaatt tgcagcatat tcatcaaggt cgctgccata tttcctttgt
     1141 caaagagcgc ggtgtggcac ccgccaccat tgattttcca ccgcgattat tccttagtgg
     1201 tggggtacga aataatgtct ggcgtgcatt ttctaaccgc aattattcaa tggctgtatg
     1261 caatctcact ggcagtaaga aagtccgcga gatgcggcat gcaacattga acagtgcgac
     1321 gcgttttatc cactttgtgg agaaccatcc tttccttata tcattgaacc gaatgtctcc
     1381 tgcgaatgtc gtttctacat tagatatcct caaacatctg tggaataaaa aactagagca
     1441 tggaacaatt taattattac ttttccgaat aatttcttca gaaatcttga catta
//
LOCUS       DADSYM010000288         1470 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2415
            SAMN08039579-rid11723173.denovo.290, whole genome shotgun sequence.
ACCESSION   DADSYM010000288 DADSYM010000000
VERSION     DADSYM010000288.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039579
            Sequence Read Archive: SRR6362533
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1470)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1470)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980162.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 03:39:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,484
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 4,347
            CDSs (with protein)               :: 4,347
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 43 of 85
            Pseudo Genes (incomplete)         :: 37 of 85
            Pseudo Genes (internal stop)      :: 19 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1470
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039579-rid11723173.denovo.290"
                     /strain="LGE2415"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8302 N 95.9628 W"
                     /collection_date="2015-08-24"
     gene            40..444
                     /gene="stpA"
                     /locus_tag="J1Y83_004341"
     CDS             40..444
                     /gene="stpA"
                     /locus_tag="J1Y83_004341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein StpA"
                     /protein_id="HBA7632414.1"
                     /translation="MSVMLQSLNNIRTLRAMAREFSIDVLEEMLEKFRVVTKERREEE
                     EQQQRELAERQEKISTWLELMKADGINPEELLGNSSAAAPRAGKKRQPRPAKYKFIDV
                     NGETKTWTGQGRTPKPIAQALAEGKSLDDFLI"
     gene            complement(491..1015)
                     /gene="ygaP"
                     /locus_tag="J1Y83_004342"
     CDS             complement(491..1015)
                     /gene="ygaP"
                     /locus_tag="J1Y83_004342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase YgaP"
                     /protein_id="HBA7632415.1"
                     /translation="MALTTISPHDAQELIARGAKLIDIRDADEYLREHIPEADLAPLS
                     VLEQSGLPAKLRREQIIFHCQAGKRTSNNADKLAAIAAPAEIFLLEDGIDGWKKAGLP
                     VAVNKSQPLPLMRQVQIAAGGLILIGVVLGYTVNSVFFLLSGFVGAGLLFAGISGFCG
                     MARLLDKMPWNQRA"
     gene            complement(1025..1324)
                     /locus_tag="J1Y83_004343"
     CDS             complement(1025..1324)
                     /locus_tag="J1Y83_004343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBA7632416.1"
                     /translation="MTELAQLQASAEQAAALLKAMSHPKRLLILCMLSGSPGTSAGEL
                     TRITGLSASATSQHLARMRDEGLIDSQRDAQRILYSIKNEAVNAIIATLKNVYCP"
BASE COUNT          411 a          344 c          341 g          374 t
ORIGIN      
        1 aaatactttt ttgttttggg ttaaaaggtt ttctttatta tgtccgtaat gttacaaagt
       61 ttaaataaca ttcgcaccct ccgtgcgatg gctcgcgaat tctccattga cgttcttgaa
      121 gaaatgctcg aaaaattcag ggttgtcact aaagaaagac gtgaagaaga agaacaacag
      181 cagcgtgaac tggcagagcg ccaggaaaaa attagcacct ggctggagct gatgaaagct
      241 gacggaatta acccggaaga gttattgggt aatagctctg ctgcggcacc acgcgctggt
      301 aaaaaacgcc agccgcgtcc ggcgaaatat aaattcatcg atgttaacgg tgaaactaaa
      361 acctggaccg gtcagggccg tacaccgaag ccaatcgctc aggcgctggc agaaggtaaa
      421 tctctcgacg atttcctgat ctaattactg aggccggatg tcgctgctat ggcgcgtccg
      481 gcgtcgcttc tcaagctcgt tggttccacg gcatcttatc taacaacctt gccattccac
      541 aaaaaccact aattcctgca aacagtaacc cagcaccaac aaagccgctt aataagaaga
      601 aaacgctatt tacggtataa cccagtacaa cgccgattaa tattaaacca cccgcggcga
      661 tctgcacctg gcgcattaac ggcaagggtt gggatttatt taccgctact ggcaatcctg
      721 cttttttcca gccatcaatc ccatcttcga gtaaaaagat ttctgcgggg gcggcaatcg
      781 ctgctaattt atcggcgtta ttactggtgc gtttacctgc ctggcagtgg aatataattt
      841 gctcgcgacg taatttagcc ggaagacctg actgttccag cacggataat ggagccagat
      901 ctgcttcagg aatatgttca cgaagatatt catcagcgtc acgaatatcg attaactttg
      961 cgccgcgtgc gattaattct tgtgcatcat gcggcgaaat ggttgtcaaa gccatagtga
     1021 ctccttacgg acaatagaca tttttcaggg tggcgataat ggcatttacc gcctcatttt
     1081 taatggaata tagaatgcgt tgggcatccc gttggctgtc gataagccct tcgtcccgca
     1141 tacgagcgag atgctgtgat gttgcagagg cacttagtcc ggtaatgcgc gtcagctctc
     1201 ccgcactggt gccgggggaa ccgctaagca tgcacagaat cagcaaccgt ttagggtggc
     1261 tcattgcttt caataaggcc gctgcctgtt cggcactagc ctgtaattgc gcgagttcag
     1321 tcatagatat ttaagtattt tctaaattaa gtaaactcta aactaaaaat gcaacatata
     1381 ccagcctcag tagcgtaaat gagagtaaaa gcgtaagctg aaactggcag gctccgctaa
     1441 aattacaacg cttaagagat aaaatctctt
//
LOCUS       DADSYM010000289         1455 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2415
            SAMN08039579-rid11723173.denovo.291, whole genome shotgun sequence.
ACCESSION   DADSYM010000289 DADSYM010000000
VERSION     DADSYM010000289.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039579
            Sequence Read Archive: SRR6362533
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1455)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1455)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980162.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 03:39:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,484
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 4,347
            CDSs (with protein)               :: 4,347
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 43 of 85
            Pseudo Genes (incomplete)         :: 37 of 85
            Pseudo Genes (internal stop)      :: 19 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1455
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039579-rid11723173.denovo.291"
                     /strain="LGE2415"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8302 N 95.9628 W"
                     /collection_date="2015-08-24"
     gene            144..>1455
                     /locus_tag="J1Y83_004344"
     CDS             144..>1455
                     /locus_tag="J1Y83_004344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001610395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase"
                     /protein_id="HBA7632417.1"
                     /translation="MLNAKKIITDSNIAFGTSGARGLVVDFTHDVCAAFTHAFLSVIE
                     SRFNFDTVSLAIDNRPSSYEIAQACAFAIKQHGFAVEYHGVIPTPALAHYSMQENIPC
                     IMVTGSHIPFERNGLKFYRPDGEITKEDEVAIVNSKYLFSPLTSLPQLEICYKAAEAY
                     IARYTSLFDKNILKGKKIGIYEHSSAGRDLYGPLFRLLGAEIISLGRSDQFVPIDTEA
                     VADDDRIKARKWSTKYKLDAIFSTDGDGDRPLVADENGEWLRGDILGLLASLELGIEA
                     LAIPVNCNSAIELSDKFKHVTRTKIGSPYVISAFNSLYQRYDAIAGFEANGGYIVLTD
                     LDYKGSKICALPTRDALLPALMLFVAARKENISNLLKKIPARYTWSDRLQNYPKEKSK
                     QLITRIRHNPNILIDLFKENLGGYLTVDNTDGVRIIIINRDILHI"
BASE COUNT          467 a          230 c          290 g          468 t
ORIGIN      
        1 attagcaacc gttcagtatc ttcaatggtc aagaagtcaa tggaagacat tcgtgttatg
       61 aaacagaatg gcattatctg gccaatagct ttagtgtata aaaatatatc gaaactccct
      121 caatttatta aaaagtaatt atcatgttaa atgctaaaaa aattattacc gatagcaata
      181 ttgcttttgg aactagtggt gcacgcggtt tggttgttga ttttacccat gatgtttgtg
      241 ctgcattcac tcatgcgttt ctttctgtaa ttgaaagcag atttaatttt gatacagttt
      301 ccttagctat tgataatcga ccgagtagtt acgaaattgc tcaggcatgc gcattcgcta
      361 tcaaacaaca tgggttcgca gttgaatatc atggtgtgat tccgactcct gcattagctc
      421 attattccat gcaggaaaat attccttgta taatggttac tgggagccat ataccttttg
      481 agcgcaatgg actaaagttt tatcgaccgg atggagaaat tactaaggaa gatgaagttg
      541 ctattgtaaa tagcaaatat ttattttccc ccttaacatc attgccccag ttagaaattt
      601 gttataaggc cgctgaggca tatattgcgc gttatacatc attgtttgat aaaaacattt
      661 tgaaaggaaa aaaaatcggg atttatgagc attcaagcgc aggtagagat ttatatggtc
      721 cattattcag actattgggc gcagaaataa tttctcttgg acgaagcgat cagtttgtac
      781 ctattgatac tgaagctgtt gcagatgatg atcgaattaa agcgcgtaaa tggtcaacta
      841 aatacaagct tgatgctata ttttctacag atggagatgg agatcgccct ttggtagctg
      901 atgaaaatgg agaatggcta cggggggata tacttgggct attagcttct ttagaattag
      961 gtattgaagc tttagctatt cctgtaaatt gtaactctgc gattgagtta tccgataagt
     1021 ttaaacatgt aacacgaact aaaataggtt caccatatgt tatttcggca tttaatagcc
     1081 tttatcaaag atatgatgct attgccggtt ttgaggctaa tggagggtat attgtattaa
     1141 ctgatctgga ttataaaggt tcaaaaatat gtgctttacc aacaagggat gctctattac
     1201 cagcattaat gctttttgtt gctgctcgta aggaaaatat atctaattta cttaaaaaaa
     1261 tacccgcaag atacacatgg tcagataggc tacaaaacta tccgaaagag aaaagcaagc
     1321 aacttatcac acggataaga cataacccca acatattgat tgatttgttt aaggaaaatc
     1381 ttggcggata tttaactgtt gacaatacag atggtgttcg gattattatc ataaatagag
     1441 atattctgca tataa
//
LOCUS       DADSYM010000290         1424 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2415
            SAMN08039579-rid11723173.denovo.292, whole genome shotgun sequence.
ACCESSION   DADSYM010000290 DADSYM010000000
VERSION     DADSYM010000290.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039579
            Sequence Read Archive: SRR6362533
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1424)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1424)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980162.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 03:39:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,484
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 4,347
            CDSs (with protein)               :: 4,347
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 43 of 85
            Pseudo Genes (incomplete)         :: 37 of 85
            Pseudo Genes (internal stop)      :: 19 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1424
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039579-rid11723173.denovo.292"
                     /strain="LGE2415"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8302 N 95.9628 W"
                     /collection_date="2015-08-24"
     gene            48..680
                     /gene="comR"
                     /locus_tag="J1Y83_004345"
     CDS             48..680
                     /gene="comR"
                     /locus_tag="J1Y83_004345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family copper-responsive transcriptional
                     repressor ComR"
                     /protein_id="HBA7632418.1"
                     /translation="MATDSTQCVKKSRGRPKVFDRDAALDKAMKLFWQHGYEATSLAD
                     LVEATGAKAPTLYAEFTNKEGLFRAVLDRYIDRFAAKHEAQLFCEEKSVASALADYFA
                     AIANCFTSKDTPAGCFMINNCTTLSPDSGDIANTLKSRHAMQERTLQQFLCQRQARGE
                     IPTHCDVTHLAEFLNCIIQGMSISAREGASLEKLMQIARTTLRLWPELLK"
     gene            complement(742..1281)
                     /locus_tag="J1Y83_004346"
     CDS             complement(742..1281)
                     /locus_tag="J1Y83_004346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HBA7632419.1"
                     /translation="MNKSMLAGIGIGVAAALGVAAVASLNVFERGPQYAQVVSATPIK
                     ETVKTPRQECRNVTVTHRRPVQDENRITGSVLGAVAGGVIGHQFGGGRGKDVATVVGA
                     LGGGYAGNQIQGSLQESDTYTTTQQRCKTVYDKSEKMLGYDVTYKIGDQQGKIRMDRD
                     PGTQIPLDSNGQLILNNKA"
BASE COUNT          360 a          390 c          346 g          328 t
ORIGIN      
        1 atttttataa taacctttca ttcacagtca ccaggtacaa cggcatcatg gcaactgact
       61 caacacaatg tgtaaaaaaa agccgtggcc gcccaaaagt gttcgacagg gatgccgcgc
      121 ttgataaggc catgaaattg ttctggcagc acggttatga agcgacttct cttgcggacc
      181 tcgttgaagc gaccggagct aaagcgccca cgctatacgc ggaatttacc aacaaagagg
      241 ggttattccg cgccgtactc gaccgctata tcgatcgttt tgccgctaag catgaagcac
      301 agctgttttg tgaagagaaa agcgtggcgt ctgcgctggc tgactatttt gctgccatcg
      361 ccaactgctt taccagcaaa gacaccccgg cgggctgctt catgatcaac aactgcacca
      421 ccctctcccc ggattctgga gatatcgcca atacgctgaa atcacgccat gcgatgcaag
      481 agcgcacttt gcagcagttt ttatgtcaac gacaagcgcg cggggaaatc ccgacccact
      541 gtgacgtgac gcatctggca gaattcctta attgtattat tcaggggatg tcgatcagcg
      601 cacgcgaagg tgcatcgctg gaaaaactga tgcagattgc ccgaacgact ttgcgtttat
      661 ggccggaact gctgaaataa gtcgcaaaaa aagcccctca gcttgagcga atgaggggct
      721 aaattgcaga gtacaacctt gttatgcttt gttattcaaa atcagctgcc cgttgctatc
      781 tagcgggatc tgcgtacccg gatcgcggtc catgcggatt ttgccctgct gatcgccaat
      841 cttataggtc acatcataac cgagcatttt ttctgacttg tcatacaccg ttttacaacg
      901 ctgttgcgta gtcgtgtaag tatcgctttc ctggagagag ccctggatct ggttacctgc
      961 atatccaccc cccagcgccc ccacaacagt ggcgacatct ttaccgcgac caccaccaaa
     1021 ctgatgccca atcacgccgc cagcaacagc gccgagcacc gacccggtaa tgcgattttc
     1081 atcctgcacc ggtcgacgat gggtcactgt aacgttgcga cactcctgac gcggtgtttt
     1141 aaccgtttcc ttgattgggg ttgcagaaac aacctgagcg tattgcgggc cacgttcaaa
     1201 cacgttcaga ctggccactg ccgctacgcc cagcgcagct gcgacaccaa tcccgatacc
     1261 cgccaacatt gacttattca cgggaatacc tccttctttg ctgttatgcc aatcatgcaa
     1321 gcgaaggaga aaagcagcta tcagacagaa gatgaaaagc gcgggaatgg ctggaaatac
     1381 ttagggattc ggaggagtct gaaggaatgc ctgatgcgac gctt
//
LOCUS       DADSYM010000291         1424 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2415
            SAMN08039579-rid11723173.denovo.293, whole genome shotgun sequence.
ACCESSION   DADSYM010000291 DADSYM010000000
VERSION     DADSYM010000291.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039579
            Sequence Read Archive: SRR6362533
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1424)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1424)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980162.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 03:39:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,484
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 4,347
            CDSs (with protein)               :: 4,347
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 43 of 85
            Pseudo Genes (incomplete)         :: 37 of 85
            Pseudo Genes (internal stop)      :: 19 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1424
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039579-rid11723173.denovo.293"
                     /strain="LGE2415"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8302 N 95.9628 W"
                     /collection_date="2015-08-24"
     gene            complement(<1..345)
                     /locus_tag="J1Y83_004347"
     CDS             complement(<1..345)
                     /locus_tag="J1Y83_004347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1398 domain-containing protein"
                     /protein_id="HBA7632420.1"
                     /translation="MDQVVVFQKMFEQVRKEQNFSWFYSELKHHRIAHYIYYLATDNI
                     RIITHDDTVLLLRGTRNLLKVSTTKNPAKIKEAALLHIRGKSTFREYCSTLAGAGVFR
                     WVTDVNHNKRSYY"
     gene            complement(566..1297)
                     /locus_tag="J1Y83_004348"
     CDS             complement(566..1297)
                     /locus_tag="J1Y83_004348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HBA7632421.1"
                     /translation="MAYYSIGDVAERCGINPVTLRAWQRRYGLLKPQRSEGGHRLFDE
                     EDIQRIEEIKRWISNGVPVGKVKALLETTSQDTQDDWSRLQEEMMSILRMANPAKLRA
                     RIISLGREYPVDQLINHVYLPVRQRLVLDHNTSRIMSSMFDGALIEYAAASLFEMRRK
                     PGKEAILMAWNVEERARLWLEAWRLSLSGWHISVLADPIESPRPELFPTQTLIVWTGM
                     APTRRQNELLQHWGEQGYKVIFHAP"
BASE COUNT          378 a          323 c          310 g          413 t
ORIGIN      
        1 gtagtaactg cgtttgttat gattaacatc agtaacccac cggaaaacgc cagcgcctgc
       61 cagtgttgaa cagtattccc gaaatgtaga ttttccgcga atatgaagca atgcggcctc
      121 ttttatttta gctgggttct tggtcgtact aacttttaac aggttcctgg ttcctcttaa
      181 taacaaaacc gtgtcatcgt gagtaataat tctgatgtta tccgtagcca gataataaat
      241 gtaatgtgca atacggtgat gttttaattc tgaataaaac caggagaagt tttgctcttt
      301 tctcacttgc tcaaacatct tttgaaaaac aacgacctga tccatcagga taataacctc
      361 ttgttaggtg tgagactgcg tagtgtgcac gatcggtttt accacttcaa tctggtctgt
      421 cctttggctg tgatatgtac aaagtgtgaa agggggatta cctgaattct cccggtgggc
      481 attttgcaac ggaccagctc cgctacaaac gctgttgtgg gttcagatta taacattctg
      541 tctaaggggc gggataaagg tgaatttagg gggcatgaaa gatgacttta taaccttgct
      601 caccccagtg ttgtaaaagt tcgttttgcc ttctcgttgg tgccatgcct gtccagacaa
      661 tcaatgtttg cgttgggaac agttccgggc gcggcgattc aatgggatca gcaagaacag
      721 aaatgtgcca tcctgacaat gataaacgcc atgcttccag ccacaatcgt gctctctctt
      781 caacattcca cgccatcaga atggcctctt taccgggctt acggcgcatt tcgaaaagtg
      841 aggccgctgc gtattcaatt aatgcgccgt caaacatact gctcataatg cgggaggtgt
      901 tgtgatcaag cacgagacgc tggcgaacag gaaggtaaac atgattaatc aattgatcta
      961 ctgggtactc tcgacccagt gaaataattc tcgcgcgtag tttggcagga ttagccatac
     1021 gaagaattga catcatctct tcttgcaagc ggctccagtc atcttgcgta tcctggctgg
     1081 tagtttccag taatgctttc actttgccta caggaacgcc attacttatc caacgcttaa
     1141 tctcttcgat gcgttgtatg tcttcttcat caaagagtcg gtgtccgcct tcactgcgct
     1201 gtggttttaa caaaccgtag cggcgttgcc aggcccggag agtgacagga ttaatcccgc
     1261 aacgttcagc aacatcacca atgctgtaat aagccacaat tcctccttgc ggtcataaat
     1321 ctccgtcgcc tctacacgac ccaataatac tttgtacaat atacgctaaa attgtacaaa
     1381 gtataaataa gatttcagct aaattggatg aaacattatt ttta
//
LOCUS       DADSYM010000292         1390 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2415
            SAMN08039579-rid11723173.denovo.294, whole genome shotgun sequence.
ACCESSION   DADSYM010000292 DADSYM010000000
VERSION     DADSYM010000292.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039579
            Sequence Read Archive: SRR6362533
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1390)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1390)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980162.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 03:39:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,484
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 4,347
            CDSs (with protein)               :: 4,347
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 43 of 85
            Pseudo Genes (incomplete)         :: 37 of 85
            Pseudo Genes (internal stop)      :: 19 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1390
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039579-rid11723173.denovo.294"
                     /strain="LGE2415"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8302 N 95.9628 W"
                     /collection_date="2015-08-24"
     gene            162..1373
                     /gene="bluF"
                     /locus_tag="J1Y83_004349"
     CDS             162..1373
                     /gene="bluF"
                     /locus_tag="J1Y83_004349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="blue light-responsive regulator BluF"
                     /protein_id="HBA7632422.1"
                     /translation="MLTTLIYRSHIRDAEPVKKIEEMVSIANRRNMRSDVTGILLFNG
                     SHFFQLLEGPEEQVQMIYRAICQDPRHYNIVELLCDYAPARRFGKAGMELFDLRLHER
                     DDVLQAVFDKGTSKFQLTYDDRALQFFRTFVLATEQSTYFEIPAEDSWLFIADGSDKE
                     LDSCTLSSNINDHFAFHPIVDPLSRRIIAFEAIVQKNEDSPSAIAVGQRKEGEIYTAD
                     LKSKALAFAMAHALKLGDKMISINLLPMTLVNEPDAVSFLLDEIKANALVPEQIIVEF
                     TESEVISRFDEFAEAVKSLKAAGISVAIDHFGAGFAGLLLLSRFQPDRIKICQELITN
                     VHKSGPRQAIIQAIMKCCTSLEIQVSAMGVATPEEWMWLESAGIEMFQGDLFSKAKLN
                     GIPSVAWPEKK"
BASE COUNT          392 a          293 c          296 g          409 t
ORIGIN      
        1 taaatgacaa cctatacagg ttttttcgcc aaaccgtctt caaccttttc cactttttat
       61 caatgtaaag aaacggttat gttttggtat taaaagaaag acacaagaag atatgtctgt
      121 taccgtctta ctctcgcctc gcccattacc ctggattgtt aatgcttacc acccttattt
      181 atcgtagcca tatacgtgac gccgaacctg tcaaaaaaat cgaagaaatg gtttcgatag
      241 caaatcgcag gaacatgcgg tctgacgtaa cagggatctt actgtttaat ggttctcatt
      301 ttttccagct tctggaaggt ccggaagaac aggttcaaat gatatatcgg gccatatgcc
      361 aggatccacg gcactataat attgttgaac tgttgtgcga ttacgcgcct gctcgccgtt
      421 ttggcaaagc aggaatggaa ttatttgatt tacgcctgca cgagcgagat gacgttttac
      481 aggccgtatt cgacaaaggc acatcaaaat ttcagctaac ttatgatgac agagcgctac
      541 aattttttcg tacttttgtc cttgcaactg aacaatcaac ctatttcgag attcctgctg
      601 aagactcctg gctttttatc gctgacggat ctgataaaga acttgattct tgtacccttt
      661 catcaaatat aaacgaccac tttgcctttc atcctattgt cgacccttta tcgcggcgga
      721 taatcgcttt tgaagccatt gtgcaaaaaa atgaagacag cccgtcagct atagcggttg
      781 ggcagcgtaa agaaggggaa atctacacag cggatctcaa aagtaaggcg cttgcattcg
      841 cgatggcaca cgcacttaag ctcggtgata aaatgatttc aatcaatcta ttacctatga
      901 ccctggttaa cgagcctgac gcagtctctt ttttacttga tgaaataaag gccaatgctc
      961 tggtgcctga acaaatcatc gttgaattta ctgaaagtga agtcatatca cggtttgatg
     1021 agtttgccga agcggttaaa tcgctaaagg ctgccggtat cagtgtagca attgatcatt
     1081 ttggcgcagg ttttgctggt ttgttactcc tgtcacgctt ccagcctgac agaattaaaa
     1141 tctgtcagga attgattacc aatgttcata aaagcggacc acggcaggca attattcagg
     1201 cgatcatgaa atgctgtaca tcacttgaaa ttcaagtctc tgctatgggt gtggcaacac
     1261 cagaagagtg gatgtggctt gaatctgcag gaattgagat gtttcaggga gatctgtttt
     1321 ccaaagctaa attgaatggt atcccttcag ttgcgtggcc ggagaaaaaa taattttcag
     1381 cacattattt
//
LOCUS       DADSYM010000293         1340 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2415
            SAMN08039579-rid11723173.denovo.295, whole genome shotgun sequence.
ACCESSION   DADSYM010000293 DADSYM010000000
VERSION     DADSYM010000293.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039579
            Sequence Read Archive: SRR6362533
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1340)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1340)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980162.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 03:39:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,484
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 4,347
            CDSs (with protein)               :: 4,347
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 43 of 85
            Pseudo Genes (incomplete)         :: 37 of 85
            Pseudo Genes (internal stop)      :: 19 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1340
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039579-rid11723173.denovo.295"
                     /strain="LGE2415"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8302 N 95.9628 W"
                     /collection_date="2015-08-24"
     gene            330..698
                     /locus_tag="J1Y83_004350"
     CDS             330..698
                     /locus_tag="J1Y83_004350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2528 family protein"
                     /protein_id="HBA7632423.1"
                     /translation="MSNIKKYIIDYDWKASIEIEIGHDVMTEEKLHQINNFWSDSEYR
                     LNKHGSVLNAVLIMLAQHALLIAISSDLNAYGVVCEFDWNDGNGQEGWPPMDGSEGIR
                     ITDIDTSGIFDSDDMTIKAA"
     gene            771..935
                     /locus_tag="J1Y83_004351"
     CDS             771..935
                     /locus_tag="J1Y83_004351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease FtsH-inhibitory lysogeny factor CIII"
                     /protein_id="HBA7632424.1"
                     /translation="MQYAIAGWPVAGCPSESLLERITRKLRDGWKRLIDILNQPGVPK
                     NGSNNYGYPD"
     gene            904..1047
                     /locus_tag="J1Y83_004352"
     CDS             904..1047
                     /locus_tag="J1Y83_004352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309204.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host cell division inhibitory peptide Kil"
                     /protein_id="HBA7632425.1"
                     /translation="MDQTIMAIQTKFTIATFIGDEKMFREAVDAYKKWILILKLRSSK
                     SIH"
     gene            1122..>1340
                     /locus_tag="J1Y83_004353"
     CDS             1122..>1340
                     /locus_tag="J1Y83_004353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309203.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host-nuclease inhibitor protein Gam"
                     /protein_id="HBA7632426.1"
                     /translation="MNAYYIQDRLEAQSWARHYQQIAREEKEAELADDMEKGLPQHLF
                     ESLCIGHLQRHGASKKAITRAFDDDVEFQ"
BASE COUNT          414 a          287 c          296 g          343 t
ORIGIN      
        1 ttaggttcta ggtttataaa agaactgaaa gaaagcaggt ctttctctcg tgtagagata
       61 agcgagtcag agttatgggg tatatcaaat aatcctgtaa accacgctca ccaacgaaac
      121 ccaaacaaaa agtggtcata acccgctcag gcggatttca ttttcacgca aacaacagaa
      181 taaacacagc gctgtgtatt cattccaacg agtgaataca cggagcaatg tcgctcgtaa
      241 ctaaacagga gccgacttgt tctgattatt ggaaatcttc tttgccctcc aagatgaggg
      301 cgatttttta tctgtgagga tatgaacaga tgtcaaacat caaaaaatac atcattgatt
      361 acgactggaa agcatcaata gaaattgaaa tcggccatga cgtaatgaca gaggaaaaac
      421 ttcaccagat taataatttc tggtcagact ctgaataccg actcaataaa cacggctctg
      481 tattaaatgc tgtattaatc atgctggcgc aacatgctct gcttatagca atttcaagcg
      541 acttaaatgc atatggtgtt gtgtgtgagt tcgactggaa tgatggaaat ggtcaggaag
      601 gatggcctcc aatggatggt agtgaaggaa taagaattac cgatatcgat acatcaggaa
      661 tatttgattc agatgatatg actatcaagg ccgcctgagc gcggcgttac cgcataccaa
      721 ttacgcttca ctcgaggcgt ttttcgttat gtataaataa ggagcacacc atgcaatatg
      781 ccattgcagg gtggcctgtt gctggctgcc cttccgaatc tttacttgaa cgaatcaccc
      841 gtaaattacg tgacggatgg aaacgcctta tcgacatact taatcagcca ggagtcccaa
      901 aaaatggatc aaacaattat ggctatccag actaaattca ctatcgccac ttttattggc
      961 gatgaaaaga tgtttcgtga ggccgtcgac gcttataaaa aatggatatt aatactgaaa
     1021 ctgagatcaa gcaaaagcat tcactaaccc cctttcctgt tttcctaatc agcccggcat
     1081 ttcgcgggcg atattttcac agctatttca ggagttcggc catgaacgct tattacattc
     1141 aggatcgtct tgaggctcag agctgggcgc gtcactacca gcagatagcc cgtgaagaga
     1201 aagaggcaga actggcagac gacatggaaa aaggcctgcc ccagcacctg tttgaatcgc
     1261 tatgcatcgg tcatttgcaa cgccacgggg ccagcaaaaa agccattacc cgtgcgtttg
     1321 atgacgatgt tgagtttcag
//
LOCUS       DADSYM010000294         1328 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2415
            SAMN08039579-rid11723173.denovo.296, whole genome shotgun sequence.
ACCESSION   DADSYM010000294 DADSYM010000000
VERSION     DADSYM010000294.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039579
            Sequence Read Archive: SRR6362533
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1328)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1328)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980162.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 03:39:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,484
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 4,347
            CDSs (with protein)               :: 4,347
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 43 of 85
            Pseudo Genes (incomplete)         :: 37 of 85
            Pseudo Genes (internal stop)      :: 19 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1328
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039579-rid11723173.denovo.296"
                     /strain="LGE2415"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8302 N 95.9628 W"
                     /collection_date="2015-08-24"
     gene            <1..>1328
                     /locus_tag="J1Y83_004354"
     rRNA            <1..>1328
                     /locus_tag="J1Y83_004354"
                     /product="23S ribosomal RNA"
BASE COUNT          335 a          295 c          412 g          286 t
ORIGIN      
        1 aaagcctcta agcatcaggt aacatcaaat cgtaccccaa accgacacag gtggtcaggt
       61 agagaatacc aaggcgcttg agagaactcg ggtgaaggaa ctaggcaaaa tggtgccgta
      121 acttcgggag aaggcacgct gatatgtagg tgaagcgact tgctcgtgga gctgaaatca
      181 gtcgaagata ccagctggct gcaactgttt attaaaaaca cagcactgtg caaacacgaa
      241 agtggacgta tacggtgtga cgcctgcccg gtgccggaag gttaattgat ggggttagcg
      301 gtaacgcgaa gctcttgatc gaagccccgg taaacggcgg ccgtaactat aacggtccta
      361 aggtagcgaa attccttgtc gggtaagttc cgacctgcac gaatggcgta atgatggcca
      421 ggctgtctcc acccgagact cagtgaaatt gaactcgctg tgaagatgca gtgtacccgc
      481 ggcaagacgg aaagaccccg tgaaccttta ctatagcttg acactgaaca ttgagccttg
      541 atgtgtagga taggtgggag gctttgaagt gtggacgcca gtctgcatgg agccgacctt
      601 gaaataccac cctttaatgt ttgatgttct aacgttggcc cgtaatccgg gttgcggaca
      661 gtgtctggtg ggtagtttga ctggggcggt ctcctcctaa agagtaacgg aggagcacga
      721 aggttggcta atcctggtcg gacatcagga ggttagtgca atggcataag ccagcttgac
      781 tgcgagcgtg acggcgcgag caggtgcgaa agcaggtcat agtgatccgg tggttctgaa
      841 tggaagggcc atcgctcaac ggataaaagg tactccgggg ataacaggct gataccgccc
      901 aagagttcat atcgacggcg gtgtttggca cctcgatgtc ggctcatcac atcctggggc
      961 tgaagtaggt cccaagggta tggctgttcg ccatttaaag tggtacgcga gctgggttta
     1021 gaacgtcgtg agacagttcg gtccctatct gccgtgggcg ctggagaact gaggggggct
     1081 gctcctagta cgagaggacc ggagtggacg catcactggt gttcgggttg tcatgccaat
     1141 ggcactgccc ggtagctaaa tgcggaagag ataagtgctg aaagcatcta agcacgaaac
     1201 ttgccccgag atgagttctc cctgagactt taagtctcct gaaggaacgt tgaagacgac
     1261 gacgttgata ggccgggtgt gtaagcgcag cgatgcgttg agctaaccgg tactaatgaa
     1321 ccgtgagg
//
LOCUS       DADSYM010000295         1301 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2415
            SAMN08039579-rid11723173.denovo.297, whole genome shotgun sequence.
ACCESSION   DADSYM010000295 DADSYM010000000
VERSION     DADSYM010000295.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039579
            Sequence Read Archive: SRR6362533
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1301)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1301)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980162.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 03:39:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,484
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 4,347
            CDSs (with protein)               :: 4,347
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 43 of 85
            Pseudo Genes (incomplete)         :: 37 of 85
            Pseudo Genes (internal stop)      :: 19 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1301
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039579-rid11723173.denovo.297"
                     /strain="LGE2415"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8302 N 95.9628 W"
                     /collection_date="2015-08-24"
     gene            <1..1191
                     /locus_tag="J1Y83_004355"
     CDS             <1..1191
                     /locus_tag="J1Y83_004355"
                     /EC_number="4.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HBA7632427.1"
                     /translation="NVHKLMDLSINKNWIDKEEYPQSAAIDLRCVNMVADLWHAPAPK
                     NGQAVGTNTIGSSEACMLGGMAMKWRWRKRMEAAGKPTDKPNLVCGPVQICWHKFARY
                     WDVELREIPMRPGQLFMDPKRMIEACDENTIGVVPTFGVTYTGNYEFPQPLHDALDKF
                     QADTGIDIDMHIDAASGGFLAPFVAPDIVWDFRLPRVKSISASGHKFGLAPLGCGWVI
                     WRDEEALPQELVFNVDYLGGQIGTFAINFSRPAGQVIAQYYEFLRLGREGYTKVQNAS
                     YQVAAYLADEIAKLGPYEFICTGRPDEGIPAVCFKLKDGEDPGYTLYDLSERLRLRGW
                     QVPAFTLGGEATDIVVMRIMCRRGFEMDFAELLLEDYKASLKYLSDHPKLQGIAQQNS
                     FKHT"
BASE COUNT          303 a          349 c          348 g          301 t
ORIGIN      
        1 aacgtccata aattgatgga tttgtcgatc aataaaaact ggatcgacaa agaagaatat
       61 ccgcaatccg ctgcaatcga cctgcgttgc gtaaacatgg ttgccgatct gtggcatgcg
      121 cctgcaccga aaaatggtca ggccgttggc accaacacca ttggttcttc cgaggcctgt
      181 atgctcggcg ggatggcgat gaaatggcgt tggcgcaagc gtatggaagc cgcaggcaaa
      241 ccaacggata aaccaaacct ggtgtgcggt ccggtacaaa tctgctggca taaattcgcc
      301 cgctactggg atgtggagtt gcgtgagatc cctatgcgcc ccggtcagtt gtttatggac
      361 ccgaaacgca tgattgaagc ctgcgacgaa aataccatcg gcgtggtgcc gactttcggc
      421 gtgacctaca ccggtaacta tgagttcccg cagccgctgc acgatgcgct ggataaattc
      481 caggccgaca ccggtatcga catcgacatg cacatcgacg ccgccagcgg tggcttcctg
      541 gcaccgttcg tcgccccgga tatcgtctgg gacttccgcc tgccgcgtgt gaaatcgatc
      601 agtgcttcag gccataaatt cggtctggct ccgctgggct gcggctgggt tatctggcgt
      661 gatgaagaag cgctgccgca ggaactggtg ttcaacgttg actacctcgg cggtcagatt
      721 ggtactttcg ccatcaactt ctcccgcccg gcgggtcagg tgattgcaca gtattatgaa
      781 ttcctgcgcc tcggtcgtga aggctatacc aaagtacaga acgcttccta ccaggttgcc
      841 gcttatctgg cggatgaaat cgccaaactg gggccgtatg agttcatctg taccggtcgc
      901 ccggacgaag gcatcccggc ggtttgcttc aaactgaaag atggtgaaga tccaggatac
      961 accctgtacg acctctctga acgtctgcgt ctgcgcggct ggcaggttcc ggccttcact
     1021 ctcggcggtg aagccaccga catcgtggtg atgcgcatta tgtgtcgtcg cggcttcgaa
     1081 atggactttg ctgaactgtt gctggaagac tacaaagcct ccctgaaata tctcagcgac
     1141 cacccgaaac tgcagggtat tgcccagcag aacagcttta aacacacctg ataacatcac
     1201 gttgtaaaaa ccgaatgccc aacctttaaa aacaggtggg tattcggcta cttattaatt
     1261 gcaataatat attacctgtc tattcatcgt gttgataaga t
//
LOCUS       DADSYM010000296         1286 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2415
            SAMN08039579-rid11723173.denovo.298, whole genome shotgun sequence.
ACCESSION   DADSYM010000296 DADSYM010000000
VERSION     DADSYM010000296.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039579
            Sequence Read Archive: SRR6362533
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1286)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1286)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980162.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 03:39:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,484
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 4,347
            CDSs (with protein)               :: 4,347
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 43 of 85
            Pseudo Genes (incomplete)         :: 37 of 85
            Pseudo Genes (internal stop)      :: 19 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1286
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039579-rid11723173.denovo.298"
                     /strain="LGE2415"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8302 N 95.9628 W"
                     /collection_date="2015-08-24"
     gene            283..546
                     /locus_tag="J1Y83_004356"
     CDS             283..546
                     /locus_tag="J1Y83_004356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HBA7632428.1"
                     /translation="MKPNIHPEYRTVVFHDTSVDEYFKIGSTIKTDREIELDGVTYPY
                     VTIDVSSKSHPFYTGKLRTVASEGNVARFTQRFGRFVSTKKGA"
     gene            546..686
                     /gene="rpmJ"
                     /locus_tag="J1Y83_004357"
     CDS             546..686
                     /gene="rpmJ"
                     /locus_tag="J1Y83_004357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HBA7632429.1"
                     /translation="MKVLNSLRTAKERHPDCQIVKRKGRLYVICKSNPRFKAVQGRKK
                     KR"
     gene            complement(756..946)
                     /locus_tag="J1Y83_004358"
                     /pseudo
     CDS             complement(756..946)
                     /locus_tag="J1Y83_004358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308353.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          370 a          287 c          270 g          359 t
ORIGIN      
        1 gagaggaata cacattgtca ggtaatcaat catgctgcaa taaatcatcg gccagtaaag
       61 tggagatagc ctccattctc gaaaaatcca tactctcagc gaaaccatca tcaatcactc
      121 atccaggcgt ttatgggagc gtcgccaatg gctgctaaca atgccagact tccccgttgc
      181 ggaaattcca catcccacaa atagtcacag tgattgggtg ttgaaatgat ccggatgagc
      241 atgtatcttt aaggttatgt tataacataa caggtaaaaa tgatgaaacc caatatccat
      301 cctgagtatc gtactgtggt gttccacgac accagcgttg atgagtactt taaaatcggc
      361 tcgactatca aaacagaccg tgagattgag ctggatggcg taacgtatcc gtacgtaaca
      421 attgatgtct cttcgaaatc gcacccgttc tatacaggga agctgagaac agtggcatca
      481 gaaggaaatg ttgcacggtt cacccaacgt tttggtcgtt ttgttagcac gaaaaagggg
      541 gcatgatgaa agtccttaac tctctgcgta ccgcaaaaga acgccatcca gactgtcaga
      601 ttgtgaagcg aaaaggacgg ctgtatgtga tttgtaaatc taatccacgt tttaaggccg
      661 ttcagggtcg taagaaaaaa cgttgattca aaattcgacg aattaacgat atttgtctga
      721 ttaataatca gaccggatta atgttggtgt atttattaca ccagcattaa ttttcctggg
      781 gatgtatctt cctgttcatt taaggtcaac tgcctgacgt ttctctccga atattccatt
      841 atattaatgt tgacttgttg accagtttcg ccctgtatgc tggcatcaac tctattttag
      901 actgaacacg ccactcagtt tcctcccttt gcgacgcagc ctgcatttac actcaaactg
      961 ttaagatgat aaatgtggta aatctgttgg tactgacata aaaacgttta cgccacagga
     1021 acggccagat ccatcggtaa ccccatcgcc gacgttcgag tgccagttag agtaacacgc
     1081 acagatgact gaatgcagtg ccctggcaaa gaggccatcg ttcctgtgac aactggcagt
     1141 cttcgtttaa cttcacttaa tttggctctt agggggctta ccggacagat gacgtactta
     1201 cacctgttta atttttcatc acttattggg atgaacaccc ataaccattt tgtgcggcat
     1261 gttaatccat taaaacacct tactga
//
LOCUS       DADSYM010000297         1280 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2415
            SAMN08039579-rid11723173.denovo.299, whole genome shotgun sequence.
ACCESSION   DADSYM010000297 DADSYM010000000
VERSION     DADSYM010000297.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039579
            Sequence Read Archive: SRR6362533
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1280)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1280)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980162.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 03:39:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,484
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 4,347
            CDSs (with protein)               :: 4,347
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 43 of 85
            Pseudo Genes (incomplete)         :: 37 of 85
            Pseudo Genes (internal stop)      :: 19 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1280
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039579-rid11723173.denovo.299"
                     /strain="LGE2415"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8302 N 95.9628 W"
                     /collection_date="2015-08-24"
     gene            <1..1269
                     /locus_tag="J1Y83_004359"
     rRNA            <1..1269
                     /locus_tag="J1Y83_004359"
                     /product="16S ribosomal RNA"
BASE COUNT          322 a          296 c          400 g          262 t
ORIGIN      
        1 ctagctggtc tgagaggatg accagccaca ctggaactga gacacggtcc agactcctac
       61 gggaggcagc agtggggaat attgcacaat gggcgcaagc ctgatgcagc catgccgcgt
      121 gtatgaagaa ggccttcggg ttgtaaagta ctttcagcgg ggaggaaggg agtaaagtta
      181 atacctttgc tcattgacgt tacccgcaga agaagcaccg gctaactccg tgccagcagc
      241 cgcggtaata cggagggtgc aagcgttaat cggaattact gggcgtaaag cgcacgcagg
      301 cggtttgtta agtcagatgt gaaatccccg ggctcaacct gggaactgca tctgatactg
      361 gcaagcttga gtctcgtaga ggggggtaga attccaggtg tagcggtgaa atgcgtagag
      421 atctggagga ataccggtgg cgaaggcggc cccctggacg aagactgacg ctcaggtgcg
      481 aaagcgtggg gagcaaacag gattagatac cctggtagtc cacgccgtaa acgatgtcga
      541 cttggaggtt gtgcccttga ggcgtggctt ccggagctaa cgcgttaagt cgaccgcctg
      601 gggagtacgg ccgcaaggtt aaaactcaaa tgaattgacg ggggcccgca caagcggtgg
      661 agcatgtggt ttaattcgat gcaacgcgaa gaaccttacc tggtcttgac atccacggaa
      721 gttttcagag atgagaatgt gccttcggga accgtgagac aggtgctgca tggctgtcgt
      781 cagctcgtgt tgtgaaatgt tgggttaagt cccgcaacga gcgcaaccct tatcctttgt
      841 tgccagcggt ccggccggga actcaaagga gactgccagt gataaactgg aggaaggtgg
      901 ggatgacgtc aagtcatcat ggcccttacg accagggcta cacacgtgct acaatggcgc
      961 atacaaagag aagcgacctc gcgagagcaa gcggacctca taaagtgcgt cgtagtccgg
     1021 attggagtct gcaactcgac tccatgaagt cggaatcgct agtaatcgtg gatcagaatg
     1081 ccacggtgaa tacgttcccg ggccttgtac acaccgcccg tcacaccatg ggagtgggtt
     1141 gcaaaagaag taggtagctt aaccttcggg agggcgctta ccactttgtg attcatgact
     1201 ggggtgaagt cgtaacaagg taaccgtagg ggaacctgcg gttggatcac ctccttacct
     1261 taaagaagcg tactttgaag
//
LOCUS       DADSYM010000298         1243 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2415
            SAMN08039579-rid11723173.denovo.300, whole genome shotgun sequence.
ACCESSION   DADSYM010000298 DADSYM010000000
VERSION     DADSYM010000298.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039579
            Sequence Read Archive: SRR6362533
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1243)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1243)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980162.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 03:39:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,484
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 4,347
            CDSs (with protein)               :: 4,347
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 43 of 85
            Pseudo Genes (incomplete)         :: 37 of 85
            Pseudo Genes (internal stop)      :: 19 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1243
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039579-rid11723173.denovo.300"
                     /strain="LGE2415"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8302 N 95.9628 W"
                     /collection_date="2015-08-24"
     gene            complement(<1..774)
                     /locus_tag="J1Y83_004360"
     CDS             complement(<1..774)
                     /locus_tag="J1Y83_004360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HBA7632430.1"
                     /translation="MRQYRLLSPMIKVVIVLFSLLFSTAQAGLVIGGTRFAYPENQSS
                     ISVELKNTSNRDMLVKVAVSPDDARQLTGTVESLPSYSETVFIATPPLFVLKPDKNTK
                     IRINRVGGSLPTDRESLFILNVAALPTLENSHSVKTDNQLQIAVRNRMKIFYRPSNLS
                     EDPNMSYQKLRWARKNEIVTVYNPGPRYVTLYNLHVDGKVIDGGMVAPFSHRQQSWCK
                     SQGVCEIAWQTLDIYNNVLPILKVKLNPSQLDFTGFAFSQ"
     gene            complement(740..1243)
                     /locus_tag="J1Y83_004361"
     CDS             complement(740..1243)
                     /locus_tag="J1Y83_004361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000688682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HBA7632431.1"
                     /translation="MSLFLFIISTSSFADDITHAGVVRIEGLITEKTCIISDESKNFT
                     VNMPDVPSSSVRSAGDVTEKVYFSITLTRCGSDVGNAYIKFTGNTVSEDASLYKLEEG
                     SVEGLALTIFDKNKGSISNDVKSIGFSLTPSVDNILHFFAAYKALKNNVQPGDANASV
                     SFIVTYD"
BASE COUNT          391 a          248 c          217 g          387 t
ORIGIN      
        1 ttgactaaat gcaaagccag taaaatccaa ctgtgaagga tttaatttta ctttcaaaat
       61 tggcaataca ttattataaa tgtccaatgt ctgccatgct atttcacaaa ccccttgcga
      121 tttacaccat gattgttggc gatggctaaa tggtgcgacc ataccaccat ctataacttt
      181 accatcaaca tgtaggttat agagtgtcac atatctgggg cctggattat aaacggtaac
      241 aatttcattt tttcgtgccc agcgtaattt ttgatatgac atattcgggt cttcagacaa
      301 atttgatggc cgatagaata ttttcatacg attgcggact gctatttgca attgattatc
      361 agttttgact gaatgactat tttccaacgt tggtaatgct gcaacattca gtataaataa
      421 actttcccgg tcggtaggta aactgccacc aacacgatta attcttattt ttgtattttt
      481 gtcaggcttc aaaacaaaca gtggcggagt tgcgataaat accgtttcag aatatgaggg
      541 caaagattca accgttcccg tcaactgacg agcatcatca ggggagactg caaccttgac
      601 taacatatct ctgtttgaag tgtttttgag ttcaacagat attgaggact gattttccgg
      661 atacgcaaaa cgcgttccac cgatcactaa gcctgcttgt gctgtagaga ataaaaggga
      721 aaataatact atgactacct taatcatagg tgacaataaa cgatactgac gcattggcat
      781 cccctggttg gacattattt tttaatgctt tgtaagccgc aaaaaaatgt aatatattat
      841 caactgatgg tgtaagtgaa aaaccgatgc ttttaacatc attactaata ctacctttat
      901 tcttatcaaa aatcgtgagt gcaagcccct ctaccgagcc ttcttccagc ttatataaac
      961 tggcatcttc tgaaactgta ttaccggtaa actttatata agcgttgcca acatcactac
     1021 cacagcgggt taacgttatg gaaaaataaa ccttttcagt aacgtctcct gcactcctta
     1081 ccgaactact gggtacgtct ggcatattaa ctgtaaaatt ttttgactca tcagaaataa
     1141 tgcaggtttt ttcggtaatt aacccttcaa tacgaactac tccggcatga gtgatatcat
     1201 cagcaaaaga acttgttgat attataaaca aaaataaact aat
//
LOCUS       DADSYM010000299         1225 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2415
            SAMN08039579-rid11723173.denovo.301, whole genome shotgun sequence.
ACCESSION   DADSYM010000299 DADSYM010000000
VERSION     DADSYM010000299.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039579
            Sequence Read Archive: SRR6362533
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1225)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1225)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980162.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 03:39:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,484
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 4,347
            CDSs (with protein)               :: 4,347
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 43 of 85
            Pseudo Genes (incomplete)         :: 37 of 85
            Pseudo Genes (internal stop)      :: 19 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1225
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039579-rid11723173.denovo.301"
                     /strain="LGE2415"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8302 N 95.9628 W"
                     /collection_date="2015-08-24"
     gene            complement(133..826)
                     /locus_tag="J1Y83_004362"
                     /pseudo
     CDS             complement(133..826)
                     /locus_tag="J1Y83_004362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
BASE COUNT          363 a          271 c          255 g          336 t
ORIGIN      
        1 tagatacccc cagatttagt tgtaaagcaa gcacactttg catttcgaga caatccaggt
       61 aaagactctt acctcccatt ttgtgatttt tatacttatg atgataagtt gaacgttggg
      121 tagtgcttcc aatcagtaga acatgtgctt ttcaatgaag gaaccgatga ctttttcgtg
      181 gatttcaacg gaacgcgaga agcagattgt tttacttacc agccgcttga tgcgggtgcg
      241 gagggtcagg ttgttacgtt caatacgttg cgtgaagatt ttgccggtca ggtatttctc
      301 cttcggcact tctctggcgt agctgcccca gtcatcgcgg ccaccatgcc gatacagaac
      361 ggagtcaaca gggctaacaa ttcccgacag gcctcatcat tccgtggccc gaaagtgtaa
      421 gccaacacac cgccagtttt agtgttctac gcataccaga gccagtgttg ttgcgccttg
      481 ttgccaacga agctccacag ctcatcaagt tcacagataa gggcaacatc agcgtgagct
      541 gactgacgca gatgttatcc gctggggcga gagcttttta atgtccgtat gacggtattg
      601 atgctgattt tcagtgttct ggcgatatca cggcccctgt gccattatgc gccatttcaa
      661 cgatcagctc cttaaagccg ggttttctgg cttcataaga gtaagtgagc tggaaaacac
      721 gtttacatga acgacaacgg aaacgctcat gctgggaaca actacgacca tgtcgataaa
      781 ctttatcgga attacagcgc ggacgatgaa cagtaacggt agccatggaa aaccttaaat
      841 accagtagat taccagaaac ttctatcaat tgaagacatt accgtttttg attacagcaa
      901 atgttttatt aattacccca ccatcgacta aatccgttag aacgtcgaat gatgccattc
      961 gtaaatacat taaaaagact gttgtaatca acgaagatgg aaaacagtgt gttaaaacta
     1021 atctgaactt cagaaaaaca gatgatggta tggtgatata tatatccgct ctgaaccgcc
     1081 acgcaattta aaataaaaat caacaaatat cgtcgatatc catatatacc atttttctgt
     1141 agttgtatta gcgtcgcata ttttatgact ttaagtcata agtcaaaaca cctgagaaaa
     1201 agcaaacaac attttcatta ccacc
//
LOCUS       DADSYM010000300         1187 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2415
            SAMN08039579-rid11723173.denovo.302, whole genome shotgun sequence.
ACCESSION   DADSYM010000300 DADSYM010000000
VERSION     DADSYM010000300.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039579
            Sequence Read Archive: SRR6362533
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1187)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1187)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980162.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 03:39:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,484
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 4,347
            CDSs (with protein)               :: 4,347
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 43 of 85
            Pseudo Genes (incomplete)         :: 37 of 85
            Pseudo Genes (internal stop)      :: 19 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1187
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039579-rid11723173.denovo.302"
                     /strain="LGE2415"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8302 N 95.9628 W"
                     /collection_date="2015-08-24"
     gene            <1..>1187
                     /locus_tag="J1Y83_004363"
     rRNA            <1..>1187
                     /locus_tag="J1Y83_004363"
                     /product="23S ribosomal RNA"
BASE COUNT          333 a          258 c          366 g          230 t
ORIGIN      
        1 gttaagcgac taagcgtaca cggtggatgc cctggcagtc agaggcgatg aaggacgtgc
       61 taatctgcga taagcgtcgg tgaggtgata tgaaccgtta taaccggcga tttccgaatg
      121 gggaaaccca gtgtgtttcg acacactatc attaactgaa tccataggtt aatgaggcga
      181 accgggggaa ctgaaacatc taagtacccc gaggaaaaga aatcaaccga gattccccca
      241 gtagcggcga gcgaacgggg aggagcccag agcctgaatc agtgtgtgtg ttagtggaag
      301 cgtctggaaa ggcgcgcgat acagggtgac agccccgtac acaaaaatgc acatattgtg
      361 agctcgatga gtagggcggg acacgtggta tcctgtctga atatgggggg accatcctcc
      421 aaggctaaat actcctgact gaccgatagt gaaccagtac cgtgagggaa aggcgaaaag
      481 aaccccggcg aggggagtga aaaagaacct gaaaccgtgt acgtacaagc agtgggagcc
      541 tcttaatggg gtgactgcgt accttttgta taatgggtca gcgacttata ttctgtagca
      601 aggttaaccg aataggggag ccgaagggaa accgagtctt aactgggcgt taagttgcag
      661 ggtatagacc cgaaacccgg tgatctagcc atgggcaggt tgaaggttgg gtaacactaa
      721 ctggaggacc gaaccgacta atgttgaaaa attagcggat gacttgtggc tgggggtgaa
      781 aggccaatca aaccgggaga tagctggttc tccccgaaag ctatttaggt agcgcctcgt
      841 gaattcatct ccgggggtag agcactgttt cggcaagggg gtcatcccga cttaccaacc
      901 cgatgcaaac tgcgaatacc ggagaatgtt atcacgggag acacacggcg ggtgctaacg
      961 tccgtcgtga agagggaaac aacccagacc gccagctaag gtcccaaagt catggttaag
     1021 tgggaaacga tgtgggaagg cccagacagc caggatgttg gcttagaagc agccatcatt
     1081 taaagaaagc gtaatagctc actggtcgag tcggcctgcg cggaagatgt aacggggcta
     1141 aaccatgcac cgaagctgcg gcagcgacgc ttatgcgttg ttgggta
//
LOCUS       DADSYM010000301         1187 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2415
            SAMN08039579-rid11723173.denovo.303, whole genome shotgun sequence.
ACCESSION   DADSYM010000301 DADSYM010000000
VERSION     DADSYM010000301.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039579
            Sequence Read Archive: SRR6362533
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1187)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1187)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980162.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 03:39:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,484
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 4,347
            CDSs (with protein)               :: 4,347
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 43 of 85
            Pseudo Genes (incomplete)         :: 37 of 85
            Pseudo Genes (internal stop)      :: 19 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1187
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039579-rid11723173.denovo.303"
                     /strain="LGE2415"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8302 N 95.9628 W"
                     /collection_date="2015-08-24"
     gene            85..807
                     /gene="sdiA"
                     /locus_tag="J1Y83_004364"
     CDS             85..807
                     /gene="sdiA"
                     /locus_tag="J1Y83_004364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SdiA"
                     /protein_id="HBA7632432.1"
                     /translation="MQDTDFFSWLRTMLLRFQRMEAAEEVYHEIELQAQQLEYDYYSL
                     CVRHPVPFTRPKVAFYTNYPESWVSYYQAKNFLAIDPVLNPENFSQGHLMWNDDLFSE
                     AQPLWEAARAHGLRRGVTQYLMLPNRALGFLSFSRCSTREIPILSDELQLKMQLLVRE
                     SLMALMRLNDEIVMTPEMNFSKREKEILKWTAEGKTSAEIAMILSISENTVNFHQKNM
                     QKKINAPNKTQVACYAAATGLI"
     gene            complement(875..1099)
                     /locus_tag="J1Y83_004365"
     CDS             complement(875..1099)
                     /locus_tag="J1Y83_004365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2594 family protein"
                     /protein_id="HBA7632433.1"
                     /translation="MSTPDFSTAENNQELANEVSCLKAMLTLMLQAMGQADAGRVMLK
                     MEKQLALIEDETQAAVFSKTVKQIKQAYRQ"
BASE COUNT          315 a          250 c          279 g          343 t
ORIGIN      
        1 taaattatat ataaatctta tttatgtgat agtttgaatt atcattataa atgatactca
       61 ctctcagggg cgttgcggtt tactatgcag gatacggatt ttttcagctg gcttcgcacg
      121 atgttgttgc gttttcagag gatggaggcc gcagaagagg tctaccatga aatagagctt
      181 caggctcagc agctggagta cgattactat tcgttatgtg ttcgccaccc ggtaccattc
      241 acgcgaccta aagtggcttt ctacaccaat taccctgagt cgtgggttag ttattatcag
      301 gcaaaaaatt ttctcgcaat tgatccggtg ctgaaccctg aaaactttag tcagggccat
      361 ttaatgtgga atgatgactt attcagcgaa gcacagccgt tatgggaagc tgcgcgcgca
      421 catggtttac gccgcggtgt cactcagtat ttaatgctgc caaatcgggc gttgggcttt
      481 ttgtcctttt cccgttgcag cacgcgcgaa atacccattc ttagtgatga actgcaatta
      541 aaaatgcagc tactggtgcg cgaaagtctg atggctctga tgcgtttaaa tgatgaaata
      601 gtgatgacgc cagagatgaa tttcagcaag cgcgaaaaag aaattctgaa atggacggcg
      661 gaaggtaaga catcagcaga gatagcgatg attttgtcaa tctctgagaa tacggtcaat
      721 ttccatcaga aaaacatgca gaaaaaaatc aatgcaccga ataagacgca ggttgcttgt
      781 tacgcggccg ctactggctt aatttgatct cttttctgtt ctgcgtgcca gatgcaaaaa
      841 ccggctgaaa ggcacgctct cagccggttt tatattactg acggtaggct tgtttaattt
      901 gcttaaccgt tttggaaaat actgcagcct gggtttcgtc ttcgatcagc gcaagctgtt
      961 tttccatctt taacatcacg cggcccgcgt cagcttgtcc catcgcctgc agcatcagcg
     1021 tcagcatcgc tttcaggcag gagacttcat ttgccagttc ttgattattc tcggcagtag
     1081 aaaaatcagg cgtactcatt tattttcctc gtcatgttgc aatgaaaatt tgcggtgaaa
     1141 aatgttaagg cggcggagta taccataagc tttgctaaaa atagcag
//
LOCUS       DADSYM010000302         1183 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2415
            SAMN08039579-rid11723173.denovo.304, whole genome shotgun sequence.
ACCESSION   DADSYM010000302 DADSYM010000000
VERSION     DADSYM010000302.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039579
            Sequence Read Archive: SRR6362533
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1183)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1183)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980162.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 03:39:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,484
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 4,347
            CDSs (with protein)               :: 4,347
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 43 of 85
            Pseudo Genes (incomplete)         :: 37 of 85
            Pseudo Genes (internal stop)      :: 19 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1183
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039579-rid11723173.denovo.304"
                     /strain="LGE2415"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8302 N 95.9628 W"
                     /collection_date="2015-08-24"
     gene            28..1173
                     /gene="espG"
                     /locus_tag="J1Y83_004366"
     CDS             28..1173
                     /gene="espG"
                     /locus_tag="J1Y83_004366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312617.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE effector EspG"
                     /protein_id="HBA7632434.1"
                     /translation="MINGLNNDSASLVLDAAMKVNSGFKKSWDEMSCAEKLLKVLSFG
                     LWNPTYSRSERQSFQELLTVLEPVYPLPNELGRVSARFSDGSSLRISVTNSESIEAEI
                     RTPDNEKITVLLESNEQNRLLQSLPIDRHMPYIQVHRALSEMDLTDTTSMRNLLGFTS
                     KLSTTLIPHNAQTDPLSGPTPFSSIFMDTCRGLGNAKLSLNGVDIPANAQMLLRDALG
                     LKDTHSSPSRNVIDHGISRHDAEQIARESSGSDNQKAEVVEFLCHPEAATAICSAFYQ
                     SFNVPALTLTHERISKASEYNAERSLDTPNACINISISQSSDGNIYVTSHTGVLIMAP
                     EDRPNEMGMLTNRTSYEVPQGVKCTIDEMVRALQPRYAASETYLQNT"
BASE COUNT          376 a          257 c          232 g          318 t
ORIGIN      
        1 attacaaacc agataggaga atctctcatg ataaatggac ttaataatga ctccgcatct
       61 ttagttttag atgctgcaat gaaagttaat tctgggttta aaaaaagctg ggatgagatg
      121 tcatgcgctg aaaagttact taaagtactt agttttggtt tatggaatcc aacgtacagt
      181 cgtagtgaaa gacaatcatt tcaagagttg ttaaccgttt tagagcctgt atatccactt
      241 cccaatgaat taggcagagt atctgctcgt ttttcagatg gctcatcctt aagaatttcc
      301 gtcactaaca gcgaaagtat tgaagccgag attcgcacac cagataatga aaagattact
      361 gtgctcctgg agtcaaacga acaaaatagg ttattacaat ctttacccat cgatcgccac
      421 atgccataca ttcaggttca tcgtgcctta tctgagatgg acctgactga tactacctca
      481 atgcgcaatc tacttggttt tacgtcaaaa ctatcaacaa ccttgattcc tcataatgct
      541 caaacagatc cgctttccgg gcctacacca ttcagctcta tctttatgga tacatgtcga
      601 ggactcggca atgcaaagct ttcactcaat ggtgttgata tacctgcaaa tgcacaaatg
      661 ttgcttcgcg atgcactagg acttaaagac acacactcat caccttcccg gaatgttata
      721 gatcatggta tttctcgcca tgatgcagag caaatagcaa gagaaagcag cggcagcgat
      781 aatcagaaag ctgaagttgt ggaattttta tgccatccag aagcagcaac ggccatatgc
      841 tcggctttct atcaatcttt caatgtgcca gccttaacgt tgacacatga aaggatctct
      901 aaagccagtg aatacaatgc ggaaagatcg ttagatacac ctaacgcttg cattaacatc
      961 agtatctctc aatcatcaga tggaaacatt tatgttacca gccataccgg ggttctgata
     1021 atggcgccag aagaccgccc caacgagatg ggcatgttga cgaacaggac ttcttatgaa
     1081 gtgccgcaag gtgtgaaatg tacaatcgat gaaatggtaa gagcgctaca accaaggtat
     1141 gccgcatctg aaacatactt acaaaacact taaaataaaa ctt
//
LOCUS       DADSYM010000303         1167 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2415
            SAMN08039579-rid11723173.denovo.305, whole genome shotgun sequence.
ACCESSION   DADSYM010000303 DADSYM010000000
VERSION     DADSYM010000303.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039579
            Sequence Read Archive: SRR6362533
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1167)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1167)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980162.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 03:39:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,484
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 4,347
            CDSs (with protein)               :: 4,347
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 43 of 85
            Pseudo Genes (incomplete)         :: 37 of 85
            Pseudo Genes (internal stop)      :: 19 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1167
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039579-rid11723173.denovo.305"
                     /strain="LGE2415"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8302 N 95.9628 W"
                     /collection_date="2015-08-24"
     gene            complement(<1..395)
                     /locus_tag="J1Y83_004367"
     CDS             complement(<1..395)
                     /locus_tag="J1Y83_004367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ail/Lom family outer membrane beta-barrel
                     protein"
                     /protein_id="HBA7632435.1"
                     /translation="MRKLYAAILSAAICLAVSGAPAWASEHQSTLSAGYLHVSTNVPG
                     SDELNGINVKYRYEFTDTLGMVTSFSYAGDKNRQLTHYSDTRWHEDSVRNRWFSVMAG
                     PSVRVNEWFSAYAMAGVAYSRVSTFSGDYL"
     gene            complement(463..>1167)
                     /locus_tag="J1Y83_004368"
     CDS             complement(463..>1167)
                     /locus_tag="J1Y83_004368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1983 domain-containing protein"
                     /protein_id="HBA7632436.1"
                     /translation="AANRIAFIDPANGNETPMFVAQGNQIFMNDVFLKRLTAPTITSG
                     GNPPAFSLTPDGKLTAKNADISGSVNANAGTLNNVTINENCQIKGKLSANQIEGDIVK
                     TVGKAFPRDSRAPERWPSGTITVRVYDDQPFDRQIVIPAVAFSGAKHEREHTDIYSSC
                     RLIVRKNGAEIYNRTALDNTLIYSGVIDMPAGHGHMTLEFSVSAWLVNNWYPTASISD
                     LLVVVMKKATAGISIS"
BASE COUNT          276 a          332 c          278 g          281 t
ORIGIN      
        1 agataatccc cggagaaagt cgacacacgg ctgtaagcca cacccgccat cgcatacgcg
       61 ctgaaccatt cattcacgcg tacagacggc cccgccatca cgctgaacca gcggttacgc
      121 acggaatctt catgccagcg ggtatcgctg taatgggtca gctggcgatt cttgtctcct
      181 gcatagctga acgacgtcac catccccagt gtgtccgtaa actcataacg gtatttcacg
      241 ttaatcccgt tcagttcatc gctgccagga acgttcgtcg agacatgaag ataccccgcg
      301 ctcagcgtgg actgatgttc agacgcccat gcaggcgcac cggatacggc cagacaaatg
      361 gctgcggaca aaatggcggc ataaagttta cgcataatta cctctcgctt ttctgcaata
      421 aaaaaggcgc catttctggc gcccgtatat gggttataaa attcagctga tactgatgcc
      481 tgcggtggct ttcttcatca ccacaaccag caaatcgctg atacttgctg tgggatacca
      541 gttattcacc agccatgctg ataccgaaaa ctccagcgtc atgtgaccgt gaccggcagg
      601 catatcaata acgccactgt aaatcagcgt attatccagc gcggtacggt tataaatttc
      661 agcaccgttt ttccgcacta tcagacggca tgaggagtaa atatcagtat gctctctctc
      721 atgcttagcg ccactgaatg ccaccgccgg aataacaatc tgccggtcaa acggctgatc
      781 gtcataaacc ctgacggtaa tggtccctga tggccaccgc tccggtgccc gggagtcccg
      841 cggaaaagcc ttacccactg ttttgactat atcgccttca atctggttgg ctgacagttt
      901 ccccttaatc tgacagttct cattaattgt gacattgttg agcgtcccgg cgttcgcatt
      961 cacactgcca ctgatatctg catttttagc ggtcagcttt ccgtccggtg tcagggaaaa
     1021 tgccggagga ttaccgccgc tggtaatggt gggagccgtc aggcgcttca ggaacacgtc
     1081 gttcatgaat atctggttgc cctgcgccac aaacatcggc gtttcattcc cgtttgccgg
     1141 gtcaataaac gcgatacgat tggcggc
//
LOCUS       DADSYM010000304         1156 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2415
            SAMN08039579-rid11723173.denovo.306, whole genome shotgun sequence.
ACCESSION   DADSYM010000304 DADSYM010000000
VERSION     DADSYM010000304.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039579
            Sequence Read Archive: SRR6362533
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1156)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1156)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980162.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 03:39:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,484
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 4,347
            CDSs (with protein)               :: 4,347
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 43 of 85
            Pseudo Genes (incomplete)         :: 37 of 85
            Pseudo Genes (internal stop)      :: 19 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1156
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039579-rid11723173.denovo.306"
                     /strain="LGE2415"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8302 N 95.9628 W"
                     /collection_date="2015-08-24"
     gene            1..1156
                     /gene="nleA"
                     /locus_tag="J1Y83_004369"
                     /pseudo
     CDS             1..1156
                     /gene="nleA"
                     /locus_tag="J1Y83_004369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309839.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="type III secretion system effector NleA"
BASE COUNT          346 a          257 c          264 g          289 t
ORIGIN      
        1 aacccccccc ttacatcttg cacacccacc acatcctgcg ccaaatcaga taccggaatc
       61 agaattgcca cctggatgtg agactggatt tgttgttaat attccagagg atatacagca
      121 acacactccg gaatgcggtg aaacaacagc tctgttgggt ttaataagag ataaaggtct
      181 gctctcaggg ttagacgcat atcttgctcc tcaccttgaa gaaggctcag caggaaaaaa
      241 agcattggat atgtttggtt tattcaatgt tactcagatg gcattagaaa tacccagcac
      301 cgttcctggt atctctggta aatatggtgt tcagatgaac attgtaaaac cagatattca
      361 cccacaaccg ggaattattt tttacagtta ttccccctac atgatgaggt gggaataaac
      421 ttcggtgcgc tcccaggacc actccagaag tttctctctc aaaatcccaa tccatggttt
      481 ggagtaacag ctcaagttgt tcgtaaccac ggcatagaac tacctatagt caaaacagat
      541 aatggatgga aactagttgg agaaacacca cttacacctg aagggcctaa agcaaattat
      601 acggaagaat gggtcatcag acctggtgag tttgacttaa agtatggcag cactccgttg
      661 cagagcactt taggtttaga atttggagct cattttaaat gggatttaga taatcctaat
      721 accaaatatg ccgttcttac caatgcactt gcgaatgcag ctggtgctgc agggggatat
      781 actctatctc aatatacagg aacagacgca atgctgagcc cccatgttgg ctcgatggcc
      841 ggacaggctt tggggcatgc tgtacaatgc tacactccag gattaaagcc agacactatt
      901 ttatggtggg caggtacgac tctggggctg gcagatttaa acaaagccga gacaggtgaa
      961 ggcagattca ctgactatcc tcgtatatgg tggcacgcaa gagaaggtgc catcttccct
     1021 aataaagaag atattgcccg tgcaacaggg gctgatatac gagctatgga agaaggtatc
     1081 cctgttggac agcggcatcc aaatccagag gatgtggtaa tcgatatcga aagcaatggc
     1141 ttaccacatc ataatc
//
LOCUS       DADSYM010000305         1149 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2415
            SAMN08039579-rid11723173.denovo.307, whole genome shotgun sequence.
ACCESSION   DADSYM010000305 DADSYM010000000
VERSION     DADSYM010000305.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039579
            Sequence Read Archive: SRR6362533
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1149)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1149)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980162.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 03:39:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,484
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 4,347
            CDSs (with protein)               :: 4,347
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 43 of 85
            Pseudo Genes (incomplete)         :: 37 of 85
            Pseudo Genes (internal stop)      :: 19 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1149
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039579-rid11723173.denovo.307"
                     /strain="LGE2415"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8302 N 95.9628 W"
                     /collection_date="2015-08-24"
     gene            117..551
                     /gene="slyA"
                     /locus_tag="J1Y83_004370"
     CDS             117..551
                     /gene="slyA"
                     /locus_tag="J1Y83_004370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416159.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SlyA"
                     /protein_id="HBA7632437.1"
                     /translation="MESPLGSDLARLVRIWRALIDHRLKPLELTQTHWVTLHNIHQLP
                     PDQSQIQLAKAIGIEQPSLVRTLDQLEEKGLISRQTCASDRRAKRIKLTEKAEPLISE
                     MEAVINKTRAEILHGISAEELEQLIKLIAKLEHNIIELQAKG"
     gene            complement(598..1065)
                     /gene="slyB"
                     /locus_tag="J1Y83_004371"
     CDS             complement(598..1065)
                     /gene="slyB"
                     /locus_tag="J1Y83_004371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein SlyB"
                     /protein_id="HBA7632438.1"
                     /translation="MIKRVLVVSMVGLSLVGCVNNDTLSGDVYTASEAKQVQNVSYGT
                     IVNVRQVQIQGGDDSNVIGAIGGAVLGGFLGNTIGGGTGRSLATAAGAVAGGVAGQGV
                     QSAMNKTQGVELEIRKDDGNTIMVVQKQGNTHFSPGQRVVLASNGSQVTVSPR"
BASE COUNT          338 a          293 c          250 g          268 t
ORIGIN      
        1 aaaatatgtc tgacggtaac caaatgcagc aatacatttg ttttagcaat acaattgctg
       61 cacactattc taaaagccgc ataatatctt agcaagctaa ttataaggag atgaaattgg
      121 aatcgccact aggttctgat ctggcacggt tggtgcgcat atggcgtgct ctgatagacc
      181 atcgcctgaa accgctggag ttaacacaaa cccattgggt tacgttacac aatatccatc
      241 agttacctcc agaccagtcg caaattcaac tggcaaaagc gattggcatc gagcagccat
      301 cactggtccg tacgctggac caactggaag aaaaagggtt aatttcgcgt caaacttgtg
      361 ccagcgatcg tcgggctaaa cgtattaaac tgacggaaaa ggcagagcca ctgatcagcg
      421 aaatggaagc cgttattaac aaaacccgcg cggaaatttt acatggcatc tccgcagagg
      481 aactggaaca actgattaag ctcatcgcaa aacttgagca taatatcatt gagttacagg
      541 ccaaagggtg aaatgaaggg ggcgtgtggt cagtaacctg accacacgca aacttattta
      601 gcgcggggaa acggtcacct gactgccatt gctggccagt acgacacgtt ggcctggaga
      661 gaaatgagtg ttcccttgtt tctgtaccac catgatggtg ttgccatcat ctttacgaat
      721 ttccagctcg acaccctgcg ttttgttcat tgcactctgt acgccctgac cggctacacc
      781 acctgcaaca gcccctgctg cagtagccag agaacgcccg gttccgccgc caatagtatt
      841 tcccaggaaa ccaccaagta cagcaccacc aattgcgccg ataacgttgg aatcatcacc
      901 gccctgaatc tgtacctgac gtacattaac gatggtgcca tagctgacat tctgtacttg
      961 tttcgcttca gaagcggtat aaacatcccc tgacagggtg tcgttattaa cacaaccgac
     1021 aagagacaga cctaccattg aaacaaccaa tacgcgttta atcattgaaa aatctcctgt
     1081 tcaccatgaa acgccacgca agcatccctc atggtcaaag tatatggcat atgtgaatcc
     1141 atggttaca
//
LOCUS       DADSYM010000306         1134 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2415
            SAMN08039579-rid11723173.denovo.308, whole genome shotgun sequence.
ACCESSION   DADSYM010000306 DADSYM010000000
VERSION     DADSYM010000306.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039579
            Sequence Read Archive: SRR6362533
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1134)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1134)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980162.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 03:39:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,484
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 4,347
            CDSs (with protein)               :: 4,347
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 43 of 85
            Pseudo Genes (incomplete)         :: 37 of 85
            Pseudo Genes (internal stop)      :: 19 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1134
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039579-rid11723173.denovo.308"
                     /strain="LGE2415"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8302 N 95.9628 W"
                     /collection_date="2015-08-24"
     gene            complement(26..724)
                     /locus_tag="J1Y83_004372"
     CDS             complement(26..724)
                     /locus_tag="J1Y83_004372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HBA7632439.1"
                     /translation="MQDIRQETLNECTRAEQSASVVLWEIDLTEVGGERYFFCNEQNE
                     KGEPVTWQGRQYQAYPIQGSGFELNGKGTSTRPTLTVSNLYGMVTGMAEDLQSLVGGT
                     VVRRKVYARFLDAVNFVNGNSEADPEQEVISRWRIEQCSELSAVSATFVLATPTETDG
                     AVFPGRIMLANTCTWTYRGDECGYSGPAVADEYDQPTSDITKDKCSKCLSGCKFRNNV
                     GNFGGFLSINKLSQ"
     gene            complement(724..1053)
                     /locus_tag="J1Y83_004373"
     CDS             complement(724..1053)
                     /locus_tag="J1Y83_004373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBA7632440.1"
                     /translation="MKTFRWKVKPGMDVTSAPSVREVRFGDGYSQRAPAGLNADLKTY
                     SVTLSVSREEATALESFLAEHGGWKAFLWTPPYGWRQIKVICAKWSSRVSMLRVEFSA
                     EFEQVVN"
BASE COUNT          249 a          370 c          267 g          248 t
ORIGIN      
        1 gctgattctg tctctgtcat gggatttact gcgaaagttt gttaatggaa aggaagccgc
       61 caaagttgcc gacattattg cggaacttac agccactcag gcatttgctg catttatcct
      121 tcgtgatatc ggacgtcggc tgatcatatt catcagcgac agccggaccg ctataaccgc
      181 actcatcacc gcgataggtc caggtacagg tgttggccag catgatacgc cccggaaaaa
      241 cagcgccatc cgtttcagtc ggtgtggcca gcacaaaggt cgcgctgacc gcgctcagtt
      301 cgctgcactg ctcgatgcgc cagcggctga tcacctcctg ctccggatcg gcttcgctgt
      361 ttccgttgac gaagttcacc gcatccagaa aacgggcata aaccttacgc ctgaccaccg
      421 ttccgccgac cagactctgc aaatcctccg ccataccggt gaccataccg tacaggttag
      481 aaaccgtcag cgtggggcgc gtactggtgc ctttgccatt cagttcaaaa ccactcccct
      541 gaatgggata cgcctgatac tgtcgcccct gccaggtgac cggctcacct ttttcgttct
      601 gctcattaca gaaaaaataa cgttcaccac cgacctctgt cagatcgatt tcccagagca
      661 ccacgctggc cgactgctcc gcacgggtgc attcattcag tgtttcctgc cggatatcct
      721 gcatcagttc accacctgtt caaactctgc gctgaactca acacgcaaca tactgacccg
      781 cgacgaccat tttgcgcaga tcacctttat ctgccgccag ccataaggcg gcgtccacag
      841 aaaggccttc cagcccccgt gctcagccag aaacgactcc agcgccgtgg cctcctcacg
      901 ggagacagac agcgtcacgc tgtacgtttt caggtcagcg ttcagcccgg caggcgcacg
      961 ctgggaatag ccatcaccaa agcgcacctc cctgacggaa ggagccgatg tcacatccat
     1021 cccgggtttc actttccagc ggaaggtttt catcgtccac ctcctgagaa caggccacca
     1081 tcacgcatct gtgcctgaat ttcatcacgg gcacccttgc gggccatgtc atac
//
LOCUS       DADSYM010000307         1120 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2415
            SAMN08039579-rid11723173.denovo.309, whole genome shotgun sequence.
ACCESSION   DADSYM010000307 DADSYM010000000
VERSION     DADSYM010000307.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039579
            Sequence Read Archive: SRR6362533
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1120)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1120)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980162.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 03:39:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,484
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 4,347
            CDSs (with protein)               :: 4,347
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 43 of 85
            Pseudo Genes (incomplete)         :: 37 of 85
            Pseudo Genes (internal stop)      :: 19 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1120
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039579-rid11723173.denovo.309"
                     /strain="LGE2415"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8302 N 95.9628 W"
                     /collection_date="2015-08-24"
     gene            complement(<1..215)
                     /locus_tag="J1Y83_004374"
     CDS             complement(<1..215)
                     /locus_tag="J1Y83_004374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415090.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1398 domain-containing protein"
                     /protein_id="HBA7632441.1"
                     /translation="MAQAAIFKEIFDQVRKDLNCELFYSELKRHNVSLYIYYLATDNI
                     HIVLENDNIVLVKGLKKVVNVKFSRNK"
     gene            516..722
                     /locus_tag="J1Y83_004375"
     CDS             516..722
                     /locus_tag="J1Y83_004375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA7632442.1"
                     /translation="MNKEQSADELSLDLIRVKNMLNSTISMSYPDVVIACIEHQVSLE
                     AFRAIEAALVKHDKNSKDYSLVVD"
BASE COUNT          302 a          212 c          248 g          358 t
ORIGIN      
        1 tgtttgtttc tggagaattt aacatttaca acctttttga gtccttttac taacactatg
       61 ttgtcgtttt ctaacacaat gtgaatatta tctgtggcta aatagtaaat ataaagtgag
      121 acattgtgac gttttagctc agaataaaat aattcacagt ttaaatcttt acgcacttga
      181 tcgaatattt ctttaaaaat ggcagcctga gccattggta aaccttccat gtgatacgat
      241 ggcgcgtagt ttgcattatc gtgtttattg tttcaatctg gtctgacctc tttgtgtttt
      301 gttgatgatt tatgtcaaat attaagcctg tttttaatga atagtgttga ttgcgtaaca
      361 aagttgagcc ttgctggcat ccaggaggga tatgcaaccg acagatgtat gtaaggtcga
      421 tgtactcaaa ctttcatact tttcctcttt tatgcagaaa gatttgaagt aatattttaa
      481 ccgctagatg acgagcaaac gcatggagcg acaaaatgaa taaagaacaa tctgctgatg
      541 aactctcgtt ggatctgatt cgtgtaaaaa atatgcttaa tagcaccatt tctatgagtt
      601 acccggatgt tgtaattgca tgtatagaac atcaggtgtc tctggaagca ttcagggcaa
      661 ttgaggcagc gttggtgaag cacgataaga attcgaagga ttattccctg gtggttgact
      721 gagcaccata actgctaatc attcaaacta tttcacctgt gacagagtca atatcgcatt
      781 ctgtcactgt caggctaata cagagctgca attcaactac tgcaatgtcc tcgtaattag
      841 gtgaatttac aatatcgtcc tgttcggatg ccggctgcat tgctgaagat gaggcattta
      901 tggttcgcat attttcccct catgctcgtc agtcctgtgc gtaggaagaa acaggacact
      961 cacactaatt tgtgtgggca tgctgtgatg tccttctgaa ttattcctat gccattatgt
     1021 aaagcgctgt atcagatgct cgtcacggct gtcaggctgt cgggtcctcc cggtgggggc
     1081 ccctgccacg gggcgggagc gtcgcggaaa aaggctagtt
//
LOCUS       DADSYM010000308         1119 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2415
            SAMN08039579-rid11723173.denovo.310, whole genome shotgun sequence.
ACCESSION   DADSYM010000308 DADSYM010000000
VERSION     DADSYM010000308.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039579
            Sequence Read Archive: SRR6362533
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1119)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1119)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980162.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 03:39:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,484
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 4,347
            CDSs (with protein)               :: 4,347
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 43 of 85
            Pseudo Genes (incomplete)         :: 37 of 85
            Pseudo Genes (internal stop)      :: 19 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1119
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039579-rid11723173.denovo.310"
                     /strain="LGE2415"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8302 N 95.9628 W"
                     /collection_date="2015-08-24"
     gene            85..1059
                     /gene="cysB"
                     /locus_tag="J1Y83_004376"
     CDS             85..1059
                     /gene="cysB"
                     /locus_tag="J1Y83_004376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012017585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator CysB"
                     /protein_id="HBA7632443.1"
                     /translation="MKLQQLRYIVEVVNHNLNVSSTAEGLYTSQPGISKQVRMLEDEL
                     GIQIFSRSGKHLTQVTPAGQEIIRIAREVLSKVDAIKSVAGEHTWPDKGSLYIATTHT
                     QARYALPNVIKGFIERYPRVSLHMHQGSPTQIADAVSKGNADFAIATEALHLYEDLVM
                     LPCYHWNRAIVVTPDHPLAGKKAITIEELAQYPLVTYTFGFTGRSELDTAFNRAGLTP
                     RIVFTATDADVIKTYVRLGLGVGVIASMAVDPVADPDLVRVDAHDIFSHSTTKIGFRR
                     STFLRSYMYDFIQRFAPHLTRDVVDAAVALRSNEEIEVMFKDIKLPEK"
BASE COUNT          287 a          261 c          268 g          303 t
ORIGIN      
        1 ttttttatta ttaaatcgta ttagtcaccc gcctggtgtg gcgaaaaaac gatgttctga
       61 tggcgtctaa gtggatggtt taacatgaaa ttacaacaac ttcgctatat tgttgaggtg
      121 gtcaatcata acctgaatgt ctcatcaacg gcggaaggac tttacacatc acaacccggg
      181 atcagtaaac aagtcagaat gctggaagac gagctaggca ttcaaatttt ttcccgaagc
      241 ggcaagcacc tgacgcaggt aacgccagca ggacaagaaa taattcgtat cgctcgtgaa
      301 gttctgtcga aagtcgatgc cataaaatcg gtcgccggag agcacacctg gccggataaa
      361 ggctcgctgt atatcgccac cacgcatact caggcacgct atgcgttacc aaacgttatc
      421 aaaggcttta ttgagcgtta tcctcgcgtt tctttgcata tgcaccaggg ctcgccgaca
      481 caaattgctg atgccgtctc taaaggcaat gccgatttcg ctatcgccac ggaagcgctg
      541 catctgtatg aagatttagt gatgctaccg tgctaccact ggaatcgggc tatagtagtc
      601 actccggatc acccgctggc aggtaaaaaa gccattacca ttgaagaact ggcgcaatat
      661 ccgttggtga catatacctt cggctttacc ggacgttcag aactggatac tgcctttaac
      721 cgcgcagggt taacgccgcg tatcgtcttc acggcaacgg atgctgacgt cattaaaact
      781 tacgtccggt tagggctggg ggtaggggtc attgccagca tggcggtgga tccggtcgcc
      841 gatcccgacc ttgtgcgtgt tgatgctcac gacatcttca gccacagtac aaccaaaatt
      901 ggttttcgcc gtagtacttt tttgcgtagt tatatgtatg atttcattca gcgttttgca
      961 ccgcatttaa cgcgtgatgt cgttgatgcg gctgtcgcat tgcgctctaa tgaagaaatt
     1021 gaggtcatgt ttaaagatat aaaactgccg gaaaaataat ttcttgcgtt attttcggca
     1081 ccttttatat agcgaaggtg ccggaatata ttctccttt
//
LOCUS       DADSYM010000309         1111 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2415
            SAMN08039579-rid11723173.denovo.311, whole genome shotgun sequence.
ACCESSION   DADSYM010000309 DADSYM010000000
VERSION     DADSYM010000309.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039579
            Sequence Read Archive: SRR6362533
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1111)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1111)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980162.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 03:39:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,484
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 4,347
            CDSs (with protein)               :: 4,347
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 43 of 85
            Pseudo Genes (incomplete)         :: 37 of 85
            Pseudo Genes (internal stop)      :: 19 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1111
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039579-rid11723173.denovo.311"
                     /strain="LGE2415"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8302 N 95.9628 W"
                     /collection_date="2015-08-24"
     gene            23..1075
                     /locus_tag="J1Y83_004377"
     CDS             23..1075
                     /locus_tag="J1Y83_004377"
                     /EC_number="5.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalomesaconate tautomerase"
                     /protein_id="HBA7632444.1"
                     /translation="MKKIPCVMMRGGTSRGAFLLAEHLPEDQTQRDKILMAIMGSGND
                     LEIDGIGGGNPLTSKVAIISRSSDPRADVDYLFAQVIVHEQRVDTTPNCGNMLSGVGA
                     FAIENGLIAATSPVTRVRIRNVNTGTFIEADVQTPNGVVEYEGSARIDGVPGTAAPVA
                     LTFLNAAGTKTGKVFPTDNQIDYFDDVPVTCIDMAMPVVIIPAEYLGKTGYELPAELD
                     ADKALLARIESIRLQAGKAMGLGDVSNMVIPKPVLISPAQKGGAINVRYFMPHSCHRA
                     LAITGAIAISSSCALEGTVTRQIVPSVGYGNINIEHPSGALDVHLSNEGQDATTLRAS
                     VIRTTRKIFSGEVYLP"
BASE COUNT          286 a          250 c          280 g          295 t
ORIGIN      
        1 tttttaaatt gagggcatta ttatgaaaaa aataccctgc gtgatgatgc gaggtggaac
       61 ctcgaggggc gcgttcctgt tagcggaaca tctacccgaa gatcaaacgc agcgcgataa
      121 aatattgatg gcaattatgg gttccggtaa cgatctggaa attgacggta ttggcggcgg
      181 taatccgctg accagtaaag tcgccattat tagccgctcc agcgatccgc gtgcagatgt
      241 cgattatctg tttgctcaag ttatcgtcca tgagcaacgt gtcgatacca cgcctaactg
      301 cggcaatatg ctgtctggcg ttggggcatt cgccattgaa aatggtttga ttgcagcgac
      361 ttcgccagtt acccgcgtac gtatccgcaa cgtcaatacg ggtacgttta tcgaagctga
      421 tgtgcaaacg ccaaatggtg ttgtcgagta cgagggtagc gccagaattg acggcgtacc
      481 gggtactgcc gcaccggttg cgctcacttt cctgaatgcc gccggaacga aaaccggaaa
      541 agtcttcccg acggataatc agattgatta ttttgacgat gtcccggtga cctgtatcga
      601 tatggcgatg ccagttgtca ttattcctgc tgaatatctg ggaaaaacag gctatgaatt
      661 accggcagag ctggatgccg acaaagcatt attagcccgc attgaatcta tccgtctaca
      721 agcgggtaaa gcaatgggct taggcgatgt cagtaatatg gttatcccta aacctgtact
      781 tatttctcca gcgcagaaag gcggggcaat taatgtgcgt tattttatgc cgcattcttg
      841 ccatcgcgct ttggcgataa ccggtgctat tgctatttcc agtagttgtg cattggaagg
      901 caccgtcacc cgacaaatcg tcccttctgt aggatacggc aatatcaata ttgaacaccc
      961 cagtggtgcg ctcgacgttc atttaagtaa tgaaggtcag gatgccacga cgttacgcgc
     1021 atctgttatt cggacgacaa gaaaaatatt ttccggtgaa gtttatcttc cctgaaaaat
     1081 tttgttgtcg tgataatgac aatcaataaa g
//
LOCUS       DADSYM010000310         1024 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2415
            SAMN08039579-rid11723173.denovo.312, whole genome shotgun sequence.
ACCESSION   DADSYM010000310 DADSYM010000000
VERSION     DADSYM010000310.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039579
            Sequence Read Archive: SRR6362533
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1024)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1024)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980162.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 03:39:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,484
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 4,347
            CDSs (with protein)               :: 4,347
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 43 of 85
            Pseudo Genes (incomplete)         :: 37 of 85
            Pseudo Genes (internal stop)      :: 19 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1024
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039579-rid11723173.denovo.312"
                     /strain="LGE2415"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8302 N 95.9628 W"
                     /collection_date="2015-08-24"
     gene            58..1002
                     /gene="leuO"
                     /locus_tag="J1Y83_004378"
     CDS             58..1002
                     /gene="leuO"
                     /locus_tag="J1Y83_004378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LeuO"
                     /protein_id="HBA7632445.1"
                     /translation="MPEVQTDHPETAELSKPQLRMVDLNLLTVFDAVMQEQNITRAAH
                     ALGMSQPAVSNAVARLKVMFNDELFVRYGRGIQPTARAFQLFGSVRQALQLVQNELPG
                     SGFEPASSERVFHLCVCSPLDSILTSQIYNHIEQIAPNIHVMFKSSLNQNTEHQLRYQ
                     ETEFVISYEDFHRPEFTSVPLFKDEMVLVASKNHPTIKGPLLKHDVYNEQHAAVSLDR
                     FASFSQTWYDTVDKQASIAYQGMAMMSVLSVVSQTHLVAIAPRWLAEEFAESLELQVL
                     PLPLKQNSRTCYLSWHEAAGRDKGHQWMEEQLVSICKR"
BASE COUNT          278 a          222 c          248 g          276 t
ORIGIN      
        1 ttatttctct gcattccaat aagggaaagg gagttaagtg tgacagtgga gttaagtatg
       61 ccagaggtac aaacagatca tccagagacg gcggagttaa gcaagccaca gctacgcatg
      121 gtcgatctca acttattaac cgttttcgat gccgtgatgc aggagcaaaa catcacccgt
      181 gccgctcatg ctctgggaat gtcgcaacct gcggtcagta acgctgttgc acgcctgaag
      241 gtgatgttta atgacgagct ttttgttcgt tatggccgtg gtattcaacc gactgctcgc
      301 gcatttcaac tttttggctc agttcgtcag gcattgcaac tagtacaaaa tgaattgcct
      361 ggttcaggtt ttgaacccgc gagcagtgaa cgtgtatttc atctttgtgt ttgcagcccg
      421 ttagacagca ttctgacctc gcagatttat aatcacattg agcagattgc gccaaatata
      481 catgttatgt tcaagtcttc attaaatcag aacactgaac atcagctgcg ttatcaggaa
      541 acggagtttg tgattagtta tgaagacttc catcgccctg aatttaccag tgtgccatta
      601 tttaaagatg aaatggtgtt ggtagccagc aaaaatcatc caacaattaa aggcccgtta
      661 ctgaagcatg atgtttataa cgaacaacac gctgcggttt cgctcgatcg tttcgcatca
      721 tttagtcaaa cctggtatga cacggtagat aagcaagcca gtatcgcgta tcagggcatg
      781 gcaatgatga gcgtacttag cgtggtgtcg caaacgcatc tggtcgctat tgcgccgcgt
      841 tggttggctg aagagttcgc tgaatcctta gagttacagg tattaccgct gccgttaaaa
      901 caaaacagca gaacctgtta tctctcctgg catgaagctg ccgggcgcga taaaggccat
      961 cagtggatgg aagagcaatt agtctcaatt tgcaaacgct aactgaatgc agaataggtc
     1021 ggac
//
LOCUS       DADSYM010000311         1001 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2415
            SAMN08039579-rid11723173.denovo.313, whole genome shotgun sequence.
ACCESSION   DADSYM010000311 DADSYM010000000
VERSION     DADSYM010000311.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039579
            Sequence Read Archive: SRR6362533
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1001)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1001)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980162.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 03:39:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,484
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 4,347
            CDSs (with protein)               :: 4,347
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 43 of 85
            Pseudo Genes (incomplete)         :: 37 of 85
            Pseudo Genes (internal stop)      :: 19 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1001
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039579-rid11723173.denovo.313"
                     /strain="LGE2415"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8302 N 95.9628 W"
                     /collection_date="2015-08-24"
     gene            complement(67..333)
                     /locus_tag="J1Y83_004379"
                     /pseudo
     CDS             complement(67..333)
                     /locus_tag="J1Y83_004379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001427161.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
     gene            490..>1001
                     /locus_tag="J1Y83_004380"
     CDS             490..>1001
                     /locus_tag="J1Y83_004380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1076 domain-containing protein"
                     /protein_id="HBA7632446.1"
                     /translation="MPLTSDIGSHSFNLGLEVFRARIAANGRGDITVGGETVSIVYDA
                     TDGSFSSSGGNGGLLSELLILGFNNGPRALSERMLSMLSDSGEAQSQEGIQSKISQYK
                     FSVCPERLQCPPESAQCPITLEIPEEGVFIKNSGDSVVCSLFDVTAFSRLVSEKSPHP
                     LTREKLTASMV"
BASE COUNT          287 a          173 c          240 g          301 t
ORIGIN      
        1 taagtattat gacgttaaaa tttaaccata cggtaaaatg attcaggaaa accggttcag
       61 catagcctag gctgaacttt tgaggatatc aacgaacgga atttttgaac atttatgagt
      121 agatcgttgt tgaagggctt caatgagcat gtcaagttca tcaactgctg gtctacgatt
      181 acgacggttt gatttaccaa tcaaagcaag tttaagtaga tatggacgag cacttttcgc
      241 cgggtttgat gtgtaattaa ttccgtatat aggtttggct gcatccagaa cactgccaag
      301 ataactaaca tcgtgactga ctgttgctgg acctacaaat gccaatcaga cggaatgttg
      361 aaaggataga atgaacagcc cgacacacaa aaaacggagt ccggctccgg tttttgtgtt
      421 gccatataag gcagatgttt gttacagcta tttaagtctg gagttcaaat taaaataggg
      481 agttttgtta tgccattaac ctcagatatt ggatcacatt catttaatct tgggctggag
      541 gtttttcgtg cccgaattgc agccaatggg cgcggagata ttacagtagg tggtgaaact
      601 gtcagtattg tgtatgatgc tactgatggg agcttttcat ccagtggcgg taatggcgga
      661 ttgctttctg agttattgat tttgggattt aataatggtc ctcgagccct tagtgagaga
      721 atgctaagta tgctttcgga ctcaggtgaa gcacaatcgc aagagggtat tcagagcaaa
      781 atatctcaat ataagttttc tgtttgtcca gagagacttc agtgcccgcc agagtctgct
      841 caatgtccca ttacattgga gataccagag gaaggggtgt ttattaagaa ttcaggggat
      901 tcagttgtat gtagtttatt tgatgttact gcattttctc ggttagttag tgaaaaatca
      961 cctcacccac tgacacgaga gaaactaacg gcatcaatgg t
//
LOCUS       DADSYM010000312          993 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2415
            SAMN08039579-rid11723173.denovo.314, whole genome shotgun sequence.
ACCESSION   DADSYM010000312 DADSYM010000000
VERSION     DADSYM010000312.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039579
            Sequence Read Archive: SRR6362533
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 993)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 993)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980162.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 03:39:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,484
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 4,347
            CDSs (with protein)               :: 4,347
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 43 of 85
            Pseudo Genes (incomplete)         :: 37 of 85
            Pseudo Genes (internal stop)      :: 19 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..993
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039579-rid11723173.denovo.314"
                     /strain="LGE2415"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8302 N 95.9628 W"
                     /collection_date="2015-08-24"
     gene            21..845
                     /gene="gadX"
                     /locus_tag="J1Y83_004381"
     CDS             21..845
                     /gene="gadX"
                     /locus_tag="J1Y83_004381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadX"
                     /protein_id="HBA7632447.1"
                     /translation="MQPLHGNCLIAYARHKYILTMVNGEYRYFNGGDLVFADASQIQV
                     DKCVENFVLVSRDTLSLFLPMLKEEALKLHVHKKVPSLLVHHCTRDIPVFQEVAQLSQ
                     NKNLRYAEMLRKRALIFALLSVFLEDTQFIPLLLNVLQPNMRTRVCTVINNNIAHEWT
                     LARIASELLMSPSLLKKKLREEGTSYSQLLTECRMQRALQLIVIYGVSIKRVAVSCGY
                     HSVSYFIYVFRNYYGMTPTEYQERSAQELPNCGSAASMAAQGIFYGTDRSAEGIRL"
BASE COUNT          289 a          183 c          222 g          299 t
ORIGIN      
        1 tatgtaatat taagtcaact atgcaaccac tacatggtaa ttgtctaatt gcgtacgcaa
       61 gacataaata tattctcacc atggttaatg gtgaatatcg ctattttaat ggcggtgatc
      121 tggtttttgc ggatgcaagc caaattcaag tggataagtg tgttgagaat ttcgtattag
      181 tttcaagaga cacgctttca ttatttctcc cgatgctcaa ggaggaggca ttaaaacttc
      241 atgtacataa aaaagttcct tcattactcg ttcatcactg taccagagat attcctgttt
      301 ttcaggaagt tgcgcaacta tcgcagaata agaatcttcg ctatgcagaa atgctacgta
      361 aaagggcatt aatctttgcg ttgttatctg tttttcttga ggatacgcag tttataccgt
      421 tacttctgaa cgtgttgcaa ccgaatatgc gaacacgagt ttgtacggtc atcaataata
      481 atatcgccca tgagtggaca ttagcccgaa ttgctagcga attgttgatg agtccaagcc
      541 tgttaaagaa aaaattgcgc gaagaaggta cgtcatattc acagttgctt actgagtgta
      601 ggatgcagcg tgctttacaa cttattgtta tatatggggt ttcaattaag cgagttgcag
      661 tatcctgtgg atatcacagc gtgtcgtatt tcatttacgt ttttcgaaat tattatggga
      721 tgacgcccac agagtatcag gagcgatcgg cgcaggaatt accgaactgt ggctcggcgg
      781 caagtatggc tgcgcaaggg attttttacg gcactgaccg ttctgcggaa ggaataagat
      841 tatagagttt tactcagaca taaaaaaaac ccggcatagg ggaccgggaa gaggatagtc
      901 tgccgtctcc agactaataa accgttataa cactccctgt tggcacggga aactttgtgc
      961 tctcagtaag ttaaatataa cttttactgg aaa
//
LOCUS       DADSYM010000313          987 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2415
            SAMN08039579-rid11723173.denovo.315, whole genome shotgun sequence.
ACCESSION   DADSYM010000313 DADSYM010000000
VERSION     DADSYM010000313.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039579
            Sequence Read Archive: SRR6362533
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 987)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 987)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980162.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 03:39:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,484
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 4,347
            CDSs (with protein)               :: 4,347
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 43 of 85
            Pseudo Genes (incomplete)         :: 37 of 85
            Pseudo Genes (internal stop)      :: 19 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..987
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039579-rid11723173.denovo.315"
                     /strain="LGE2415"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8302 N 95.9628 W"
                     /collection_date="2015-08-24"
     gene            238..>987
                     /gene="cif"
                     /locus_tag="J1Y83_004382"
     CDS             238..>987
                     /gene="cif"
                     /locus_tag="J1Y83_004382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000652080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector Cif"
                     /protein_id="HBA7632448.1"
                     /translation="MKDITLPPPTSASCLTGAISVNTEAVLSPMQHTSALHVRDFASL
                     CSQNLKANVLLNSDDHEVPIHQKNPAAIMQNIDSNIKQMATDWGMSIEEVEVIIGREK
                     GIVEPSCGVTANAIMKLFLDKDGFSYCFENEQTLSLEQLQERLSCMPECKSFVLRVND
                     GALGHAYIVDIPKGENSCRPAFLYQSDLGEGVTRKLRFEDWMTHKALTPILLDDICNY
                     FSCMSQNKTDLEQIATLFDIDGNVKMLRKENI"
BASE COUNT          296 a          190 c          227 g          274 t
ORIGIN      
        1 taagtaaaat ttagtaattt tatccagtgt agtggatttg ttgcatggat ggagttggta
       61 aaaagtggtg tggctggcaa tccaggccgc gtcacagaaa tggacaatgc cgctgagaga
      121 ctggcgaatg gcatacaggc accagaaaaa tttcagacat gacataatgc ccatgccaga
      181 agttcctttt gcacctcgcc tgtatttata gataaacata gagtgaatga atgagatatg
      241 aaagacatta cccttccccc cccgacgtcc gcgtcctgtc tgacaggggc catatctgta
      301 aatactgaag ctgtattatc tcccatgcaa cacacttcag ccttacatgt aagagatttt
      361 gcttccctgt gctcacagaa cctcaaagct aatgtattgc taaatagtga tgaccacgaa
      421 gtacctatac atcagaaaaa tcctgctgca ataatgcaaa atatcgactc taacatcaaa
      481 cagatggcaa cagactgggg gatgtcgatt gaggaggttg aggttattat agggcgagag
      541 aaaggcattg tggaaccctc ctgcggagtt accgctaatg ctattatgaa actatttctg
      601 gacaaggatg gcttcagtta ctgctttgaa aatgaacaga cactatcgct cgagcagctt
      661 caggaacgcc tgtcctgtat gcctgaatgt aagagctttg tattacgtgt taatgatggt
      721 gcgcttggtc atgcttacat tgtcgatatt cccaaaggag aaaactcttg tcgtcctgca
      781 ttcttgtatc agtcagattt aggagagggc gtcaccagaa agttaagatt tgaggactgg
      841 atgacgcata aagcattgac tccgattttg ctggatgata tttgtaatta cttctcctgc
      901 atgtctcaaa ataagacaga tttggagcag attgcaacgt tatttgatat tgatgggaat
      961 gttaaaatgt tacgaaagga aaatatt
//
LOCUS       DADSYM010000314          982 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2415
            SAMN08039579-rid11723173.denovo.316, whole genome shotgun sequence.
ACCESSION   DADSYM010000314 DADSYM010000000
VERSION     DADSYM010000314.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039579
            Sequence Read Archive: SRR6362533
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 982)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 982)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980162.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 03:39:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,484
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 4,347
            CDSs (with protein)               :: 4,347
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 43 of 85
            Pseudo Genes (incomplete)         :: 37 of 85
            Pseudo Genes (internal stop)      :: 19 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..982
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039579-rid11723173.denovo.316"
                     /strain="LGE2415"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8302 N 95.9628 W"
                     /collection_date="2015-08-24"
     gene            complement(<1..266)
                     /locus_tag="J1Y83_004383"
     CDS             complement(<1..266)
                     /locus_tag="J1Y83_004383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HBA7632449.1"
                     /translation="MKGLLALLISSMVLPAHAGIVIYGTRIIYPAEHKEVMVQLMNQG
                     NRSSLMQAWIDDGDTSLPPEKIQVPFMLTPPVAKIGANSGQQVK"
     gene            complement(347..889)
                     /locus_tag="J1Y83_004384"
     CDS             complement(347..889)
                     /locus_tag="J1Y83_004384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HBA7632450.1"
                     /translation="MKRSIIAAAVFSSFFMSAGVFAADVDTGTLTIKGNIAESPCKFE
                     AGGDSVSINMPTVPTTVFEGKAKYSTYDGAVGVTSSMLKISCPKEVAGVKLSLITNDK
                     ITGNDKAIASSNDTVGYYLYLGDNSDVLDVSAPFNIESYKTADGQYAIPFKAKYLKLT
                     DNSVNSGDVLSSLIMRVAQD"
BASE COUNT          285 a          217 c          184 g          296 t
ORIGIN      
        1 atttttactt gctgcccgga atttgcccct atttttgcca ctggtggcgt taacatgaaa
       61 ggaacctgaa ttttttctgg tggtaatgac gtatcgccat catcaatcca cgcctgcatc
      121 agcgaagaac ggtttccctg gttcatcaac tgcaccatca cttctttgtg ctctgccggg
      181 taaataatac gcgtcccgta gataacaata ccggcatgtg caggaaggac catagaagaa
      241 atgagtaaag ctaataatcc tttcatctta atgcctctta aattaaaagg ccgccatgga
      301 gatagcggcc atgttaatta cctgaagaat cttgcaattt tatttattaa tcctgcgcca
      361 cacgcataat cagggaagat aacacatcac ctgaattcac tgagttatct gtcagtttca
      421 ggtattttgc tttaaacgga atggcatatt gaccgtccgc tgttttataa ctctcgatgt
      481 tgaaaggtgc agaaacatcc aggacatcgc tgttatcacc taaatagaga taataaccca
      541 cggtatcgtt gctactggct atcgccttat cgtttccggt tattttatcg ttggtaatca
      601 acgagagttt tacaccagca acttctttcg gacagctaat ttttaacatg ctgctggtta
      661 caccgactgc accatcatag gtcgagtatt tagctttacc ttcaaagacc gtagttggta
      721 cagttggcat attaatactt actgaatcgc cacctgcttc gaatttacac ggagattctg
      781 caatattccc tttaatggtt aatgttccgg tatcaacgtc tgcggcaaat accccagcgc
      841 tcataaaaaa agaagaaaaa acagcggcag caataattga acgtttcata atatgctcct
      901 taataaaatc tatttttggt tgttaagagt ctgtatctct tggaaacaaa tttctcacac
      961 caaaaaagtt gccgcaatgt aa
//
LOCUS       DADSYM010000315          941 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2415
            SAMN08039579-rid11723173.denovo.317, whole genome shotgun sequence.
ACCESSION   DADSYM010000315 DADSYM010000000
VERSION     DADSYM010000315.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039579
            Sequence Read Archive: SRR6362533
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 941)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 941)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980162.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 03:39:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,484
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 4,347
            CDSs (with protein)               :: 4,347
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 43 of 85
            Pseudo Genes (incomplete)         :: 37 of 85
            Pseudo Genes (internal stop)      :: 19 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..941
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039579-rid11723173.denovo.317"
                     /strain="LGE2415"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8302 N 95.9628 W"
                     /collection_date="2015-08-24"
     gene            <1..212
                     /locus_tag="J1Y83_004385"
     CDS             <1..212
                     /locus_tag="J1Y83_004385"
                     /inference="COORDINATES: protein motif:HMM:NF014467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HBA7632451.1"
                     /translation="FQKWNGTAWVKDTEAEKLFRIREAEETKNSLMQVASEHIAPLQD
                     AVDRNGERNLVAGSLEKVSGVAEPC"
     gene            complement(354..>941)
                     /locus_tag="J1Y83_004386"
     CDS             complement(354..>941)
                     /locus_tag="J1Y83_004386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HBA7632452.1"
                     /translation="SRIYAYCRVSTLEQTTKNQRREIESAGFNIKSQQIIEEQISGSA
                     ATSERPGFNQLLDRLKRGDALIVTKLDRLGCNPMDISKTVEQLTKAGIKVHCLALGGV
                     DLTSPPGKMMMQVISAVAEFERDLLLERTHSGILRARGAGKRFGRPPVLNDEQIQRVL
                     EQIKSGINISDIAQEFKTSRQTILRAKAKNQTPDI"
BASE COUNT          250 a          198 c          216 g          277 t
ORIGIN      
        1 agtttcagaa gtggaacggc acagcctggg tgaaggatac ggaagcagaa aaactgttcc
       61 ggatccggga ggcggaagaa acaaaaaaca gcctgatgca ggtagccagt gagcatatag
      121 cgccgcttca ggatgctgtg gatcgcaacg gagaaagaaa ccttgttgct ggaagcctgg
      181 aaaaagtatc gggtgttgct gaaccgtgtt gatacatcaa ctgcaccgga tattgaatgg
      241 ccagccctac cgtagggtaa gatatataaa ttctataatt agaagtatct ttccatttaa
      301 ggctaggaag ggggggcttg gaaaacgtaa ggaatctcac accgagatta tttttatata
      361 tcaggtgtct gattttttgc tttagctctt aaaatggttt gccgcgaggt tttgaattct
      421 tgggcaatgt cacttatatt tatacctgac ttaatttgct ctagtaccct ctgtatttgt
      481 tcatcattta acacaggtgg gcgaccaaat cgctttcctg cgccccgtgc tcttaatatc
      541 cctgaatgag tacgctcaag taaaaggtct cgctcaaatt cagcgactgc tgaaattact
      601 tgcatcatca tttttccagg tggactggtc aggtcaacgc cccccaatgc taagcaatgc
      661 actttgatac ctgctttggt cagttgttcc accgttttac tgatatccat tggattacaa
      721 ccgaggcgat ccagttttgt gacaatcaat gcatcacctc ttttcagtcg atcaagcaac
      781 tggttaaaac caggacgctc actggttgct gctgagccgc taatttgttc ttcaattatt
      841 tgctgagatt tgatgttaaa acctgcactt tcgatttccc gacgttgatt tttggttgtt
      901 tgttccagag ttgatacccg acagtaagca taaattcgag a
//
LOCUS       DADSYM010000316          930 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2415
            SAMN08039579-rid11723173.denovo.318, whole genome shotgun sequence.
ACCESSION   DADSYM010000316 DADSYM010000000
VERSION     DADSYM010000316.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039579
            Sequence Read Archive: SRR6362533
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 930)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 930)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980162.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 03:39:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,484
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 4,347
            CDSs (with protein)               :: 4,347
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 43 of 85
            Pseudo Genes (incomplete)         :: 37 of 85
            Pseudo Genes (internal stop)      :: 19 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..930
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039579-rid11723173.denovo.318"
                     /strain="LGE2415"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8302 N 95.9628 W"
                     /collection_date="2015-08-24"
     gene            complement(88..>930)
                     /locus_tag="J1Y83_004387"
     CDS             complement(88..>930)
                     /locus_tag="J1Y83_004387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HBA7632453.1"
                     /translation="SVIVVHNSMKLYSQIRKRAPQARVVIHMHNAFEPKLLEQNVKMI
                     VPSLYLKKYYQSYLANADIEIVPNGIDLETYQSTFQPITRSELNISAEEKIIFYAGRI
                     VPDKGILLLMQSFEKLAAAHKNLKLVVIGDYTEMSKSDKGAYQRNVREIANRLKDRCI
                     MLGSIPPEKMHCYYPLADLVVIPSQFQEPFCMVAIEAMGAGKPVLVSTRGGMTEFVKE
                     NTTGFHLKEPMTADSISSDILKTLANPELTAVAKQGQDFVFDHYSWDGVTQRFEEVIH
                     NWFE"
BASE COUNT          283 a          194 c          151 g          302 t
ORIGIN      
        1 caaatttatt aggcgtttac gccggcttat tattagcgat gaaaaacaag taaaaaataa
       61 aaaggctgca taatgcggcc tttttattta ttcaaaccaa ttatgaataa cctcttcaaa
      121 tctttgagtt acgccatccc aactgtagtg atcaaacaca aaatcctgac cttgctttgc
      181 aactgctgtt aattcaggat tcgctaacgt ttttaaaata tcgctgctta tagagtctgc
      241 agtcataggt tcttttaaat ggaatcccgt agtattttct ttgacaaatt ccgtcatccc
      301 cccacgcgta ctcactaaaa caggcttacc agcccccata gcttcaattg ccaccatgca
      361 aaatggttcc tggaactggg aaggaatgac gactaaatct gccagagggt aataacaatg
      421 catcttttca ggcggtatgc ttcctaacat aatgcaacga tccttcaaac ggtttgcaat
      481 ttccctgaca ttgcgttgat atgcaccttt gtcactttta ctcatttctg tataatcacc
      541 aatcacaact aattttaaat ttttgtgagc agctgccaat ttttcgaatg attgcatcaa
      601 cagtagtatg cctttgtcag ggacgatacg cccagcataa aaaataattt tctcttcagc
      661 ggagatatta agttcgcttc tagtgatcgg ctgaaaagta gattgatagg tttctaaatc
      721 aattccattt ggtacaatct caatatctgc atttgctaaa taagattggt aatacttttt
      781 caagtataaa ctaggcacta tcattttaac attttgctca agcaattttg gttcaaatgc
      841 attatgcata tgtataacca ctcttgcctg aggtgctcgc ttacgaattt gagaatatag
      901 cttcatgcta ttatgaacta caataacact
//
LOCUS       DADSYM010000317          929 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2415
            SAMN08039579-rid11723173.denovo.319, whole genome shotgun sequence.
ACCESSION   DADSYM010000317 DADSYM010000000
VERSION     DADSYM010000317.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039579
            Sequence Read Archive: SRR6362533
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 929)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 929)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980162.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 03:39:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,484
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 4,347
            CDSs (with protein)               :: 4,347
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 43 of 85
            Pseudo Genes (incomplete)         :: 37 of 85
            Pseudo Genes (internal stop)      :: 19 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..929
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039579-rid11723173.denovo.319"
                     /strain="LGE2415"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8302 N 95.9628 W"
                     /collection_date="2015-08-24"
     gene            5..>929
                     /locus_tag="J1Y83_004388"
     CDS             5..>929
                     /locus_tag="J1Y83_004388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415890.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA7632454.1"
                     /translation="MTVKISGVLKDGAGKPVQNCTIQLKARRNSTTVVVNTVASENPD
                     EAGRYSMDVEYGQYSVTLLVEGFPPSHAGTITVYEDSQPGTLNDFLGAMTEDDARPEA
                     LRRFELMVEEVARNASVVAQNTAAAKKSASDASTSASEAATRATDAADSARAASTSAG
                     QAASSAQSASSSAGTASTKATEASKSAAAAESSKSAAATSAGAAKTSETNAAASEQSA
                     ATSASTATTKASEAATSARDASASKEAAKSSETNASSSASSAASSATAAGNSAKAAKT
                     SETNARSSETAAAQSASAAAGSKTAAASSASA"
BASE COUNT          243 a          261 c          275 g          150 t
ORIGIN      
        1 gaatatgacg gttaagattt caggtgtact gaaagatgga gcaggcaaac cggtacaaaa
       61 ctgtaccatt cagctaaagg ccagacgcaa cagcaccacg gtggtggtga acacggtggc
      121 ctcagaaaat ccggatgaag ccgggcgtta cagcatggac gttgagtatg gccagtacag
      181 cgtcaccctg ctggttgaag gttttccgcc ttcgcatgcc gggaccatca ccgtgtatga
      241 agattctcaa cccggtacgc tgaatgattt tctcggcgca atgacggagg atgatgcccg
      301 tccggaggca ctgcgtcgtt ttgaactgat ggtggaagag gtggcgcgta acgcgtccgt
      361 ggtggcacag aacacggcag ccgcaaagaa gtcagccagt gatgccagca catctgccag
      421 tgaggcggca acccgtgcga ctgatgctgc agattcagca cgtgcagcca gcacgtcagc
      481 cggacaggcc gcgtcgtcgg ctcagtcagc gtcttccagc gcaggaacgg catcaacaaa
      541 ggccactgaa gcctcaaaaa gtgctgccgc tgcagagtcc tcaaaaagcg cggcggctac
      601 cagtgccggt gcggcgaaaa cgtcagaaac gaatgcggca gcgtcagaac aatcagccgc
      661 cacttctgca tccaccgcga ccacgaaagc gtcagaagct gccacctcag ccagggatgc
      721 gtcggcttca aaagaggcgg caaaatcatc agaaacgaac gcatcatcaa gcgccagtag
      781 tgcagcttcc tcggcaacgg cggcaggaaa ttccgcgaag gcggcaaaga cgtctgagac
      841 aaacgccagg tcttctgaaa cggcagcagc acagagtgcc tcagctgcgg caggctcaaa
      901 aacagcggct gcatcatctg ccagtgccg
//
LOCUS       DADSYM010000318          915 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2415
            SAMN08039579-rid11723173.denovo.320, whole genome shotgun sequence.
ACCESSION   DADSYM010000318 DADSYM010000000
VERSION     DADSYM010000318.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039579
            Sequence Read Archive: SRR6362533
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 915)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 915)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980162.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 03:39:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,484
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 4,347
            CDSs (with protein)               :: 4,347
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 43 of 85
            Pseudo Genes (incomplete)         :: 37 of 85
            Pseudo Genes (internal stop)      :: 19 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..915
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039579-rid11723173.denovo.320"
                     /strain="LGE2415"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8302 N 95.9628 W"
                     /collection_date="2015-08-24"
     gene            <1..>915
                     /locus_tag="J1Y83_004389"
     CDS             <1..>915
                     /locus_tag="J1Y83_004389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="host specificity protein J"
                     /protein_id="HBA7632455.1"
                     /translation="YPNTALVGVQVDSEQFGSQQVSRNYHLRGRILQVPSNYDPEKRT
                     YSGIWDGTLKPAYSNNIAWCLWDMLTHPRYGMGKRLGAADVDKWALYVIGQYCDQSVP
                     DGSGGTEPRITCNAYLTTQRKAWDVLSDFCSAMRCMPVWNGQTLTFVQDRPSDKVWTY
                     NRSNVVMPDDGAPFRYSFSALKDRHNAVEVNWIDPNNGWETATELVEDTQAIARYGRN
                     VTKMDAFGCTSRGQAHRAGLWLIKTELLETQTVDFSVGAEGLRHVPGDVIEICDDDYA
                     GISTGGRVLAVNSQTRTLTLDREITLPS"
BASE COUNT          197 a          243 c          300 g          175 t
ORIGIN      
        1 gctacccgaa cacggcactg gtcggcgtac aggtggattc ggagcagttc ggcagccagc
       61 aggtgagccg taattatcat ctgcgcggac gcattctgca ggtgccgtcg aattacgacc
      121 cggaaaaacg cacatacagc ggtatctggg acggaacgct taaaccggca tacagcaaca
      181 acatagcctg gtgtctgtgg gatatgctga cccatccgcg ttatggcatg gggaaacgtc
      241 ttggtgcagc ggatgtggat aaatgggcgc tgtatgtcat cggccagtac tgcgaccagt
      301 cggtgccgga cggctctggt ggcacggagc cgcgcatcac ctgtaatgcg tacctgacca
      361 cacagcgcaa ggcgtgggat gtgctcagtg atttctgctc ggcgatgcgc tgtatgccgg
      421 tatggaacgg gcagacgctg acgttcgtgc aggaccgacc gtcggataag gtgtggacct
      481 ataaccgcag taatgtggtg atgccggatg atggcgcgcc gttccgctac agcttcagcg
      541 ccctgaagga ccgccataat gccgttgagg tgaactggat tgacccgaat aacggctggg
      601 agacggcgac agagcttgtg gaggacacgc aggccattgc ccgttacggt cgtaacgtca
      661 cgaagatgga tgcctttggc tgtaccagcc gggggcaggc acaccgcgcc gggctgtggc
      721 tgattaaaac agaactgctg gaaacgcaga ccgtggactt cagcgtgggt gccgaagggc
      781 ttcgccatgt gccgggcgat gtcattgaaa tctgtgatga tgactatgcc ggtatcagca
      841 ccggcgggcg cgtgctggcg gtaaacagcc agacccggac gctgacgctc gaccgtgaaa
      901 tcacgctgcc atcct
//
LOCUS       DADSYM010000319          905 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2415
            SAMN08039579-rid11723173.denovo.321, whole genome shotgun sequence.
ACCESSION   DADSYM010000319 DADSYM010000000
VERSION     DADSYM010000319.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039579
            Sequence Read Archive: SRR6362533
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 905)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 905)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980162.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 03:39:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,484
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 4,347
            CDSs (with protein)               :: 4,347
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 43 of 85
            Pseudo Genes (incomplete)         :: 37 of 85
            Pseudo Genes (internal stop)      :: 19 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..905
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039579-rid11723173.denovo.321"
                     /strain="LGE2415"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8302 N 95.9628 W"
                     /collection_date="2015-08-24"
     gene            395..601
                     /locus_tag="J1Y83_004390"
     CDS             395..601
                     /locus_tag="J1Y83_004390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001524508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division inhibitor protein"
                     /protein_id="HBA7632456.1"
                     /translation="MVHQHYGTQTVNRGAVMPGMLVKHKDGTWTASANLRGRLYLHRG
                     IERTYTRDLLVEVFLDGRGNGLNH"
     gene            677..>905
                     /locus_tag="J1Y83_004391"
     CDS             677..>905
                     /locus_tag="J1Y83_004391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309203.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host-nuclease inhibitor protein Gam"
                     /protein_id="HBA7632457.1"
                     /translation="MNAYYIQDRLEAQSWARHYQQIAREEKEAELADDMEKGLPQHLF
                     ESLCIDHLQRHGASKKAITRAFDDDVEFQERM"
BASE COUNT          247 a          226 c          211 g          221 t
ORIGIN      
        1 ttatcgctat gcgatgcaag tcacaaaatt aattcttttt gctatcaaac atttaatatc
       61 aaaacacatc agtcaatagc aataagtatt gatatcacca atagcaatag ctattatcat
      121 catatcgcaa caacacaacg atacggcaac cacctgattc accgttgcga tgaccgctta
      181 gatccgtagc ttgaatttca gcaggctccg ggaagtgcga ggggtgaagc ggacgcgtga
      241 acgtcggtgt gaccagctga aattaactca acatttcata cctcagtcgc ttcaacgagg
      301 cggcttagtt atgacaaccg gcggccatcc accgcctgaa tacgcgcaga agtctttata
      361 tgttcagcag cccagcttac gggcaggagt ttttatggtt catcaacatt acggaacgca
      421 gaccgttaat cgcggtgcgg tcatgccagg aatgctggtc aaacacaaag atggtacctg
      481 gacagcatca gctaatttac gcggacggct atatctgcat cgcggcatcg agcgcactta
      541 tacccgtgat ttgctcgtgg aagtttttct cgacggacga ggcaacggcc tgaatcacta
      601 atcccctttc ctgttttcct aatcagcctg gcatttcgcg ggcgatattt tcacagccat
      661 tttcaggagt tcagccatga acgcttatta cattcaggat cgtcttgagg ctcagagctg
      721 ggcgcgtcac taccagcaga ttgcccgtga agagaaagag gcagaactgg cagacgacat
      781 ggaaaaaggt cttccacagc acctgtttga atcactctgc atcgatcatt tgcaacgcca
      841 cggggccagc aaaaaagcca ttacccgtgc atttgatgac gatgttgagt ttcaggagcg
      901 catgg
//
LOCUS       DADSYM010000320          897 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2415
            SAMN08039579-rid11723173.denovo.322, whole genome shotgun sequence.
ACCESSION   DADSYM010000320 DADSYM010000000
VERSION     DADSYM010000320.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039579
            Sequence Read Archive: SRR6362533
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 897)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 897)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980162.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 03:39:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,484
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 4,347
            CDSs (with protein)               :: 4,347
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 43 of 85
            Pseudo Genes (incomplete)         :: 37 of 85
            Pseudo Genes (internal stop)      :: 19 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..897
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039579-rid11723173.denovo.322"
                     /strain="LGE2415"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8302 N 95.9628 W"
                     /collection_date="2015-08-24"
     gene            61..627
                     /locus_tag="J1Y83_004392"
     CDS             61..627
                     /locus_tag="J1Y83_004392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000032634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HBA7632458.1"
                     /translation="MSKFAKTAIAAAMVMGAVVSSSAFAAGNNGTARFYGTIEDSPCS
                     IVPDDHKLEVDMGDIGAAKLTGGGTTTPKDFQIRLQDCVFTTETNMETTFTGTNYAGP
                     ANADNYALFNVDSGVAMNHVSLVIGDTHGKGYKVGEKIVQPIVMDTSTSKGKDKQTLN
                     FKAWLVGETDAPDLGQFETLTTFQITYL"
BASE COUNT          244 a          209 c          218 g          226 t
ORIGIN      
        1 catatattaa ttttatgtca ggtgcttata ttataactct taatgattta aggacaaata
       61 atgagtaagt ttgccaaaac agccattgct gcggcgatgg tcatgggcgc agttgtttcc
      121 agttctgctt ttgcagcagg caacaacggt accgcgcgct tctacggcac cattgaagac
      181 tccccgtgct ctatcgttcc agacgatcac aaactggaag tcgatatggg tgacatcggt
      241 gcggctaaac tgaccggcgg tggcactacc acaccgaaag atttccagat ccgtttacag
      301 gattgcgtgt ttaccactga aacaaacatg gagaccacgt tcaccggcac taactacgcg
      361 gggccagcca acgcagataa ctatgcgtta ttcaatgttg acagcggtgt tgccatgaac
      421 cacgtgagcc tggtaatcgg cgatacccat ggcaaaggtt ataaagtggg cgaaaagatt
      481 gttcagccaa tcgtgatgga tacttcaacc agcaagggta aagacaagca aacgctgaac
      541 tttaaagcct ggctggtggg tgaaaccgat gcgccggatc tcggccagtt cgaaaccctg
      601 accacattcc agattaccta tctctgatgt gacaacccca cagcgttctg ctgtggggtt
      661 attcaacacg caacagattt acgactgttt cacaaacgga tatgtgtatg cctaatcact
      721 caaattttcg gctgcgggga atcgcctgct atattgcgct ggcaatctct ggtgggtcag
      781 tcaatgcatg ggctgatgat tcaattcaat ttgacccccg tttccttgag ttaaagggcg
      841 atacgaaaat tgatctcggt aagttttcaa aaaaagggta tgtcgacgcg ggtaaat
//
LOCUS       DADSYM010000321          892 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2415
            SAMN08039579-rid11723173.denovo.323, whole genome shotgun sequence.
ACCESSION   DADSYM010000321 DADSYM010000000
VERSION     DADSYM010000321.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039579
            Sequence Read Archive: SRR6362533
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 892)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 892)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980162.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 03:39:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,484
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 4,347
            CDSs (with protein)               :: 4,347
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 43 of 85
            Pseudo Genes (incomplete)         :: 37 of 85
            Pseudo Genes (internal stop)      :: 19 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..892
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039579-rid11723173.denovo.323"
                     /strain="LGE2415"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8302 N 95.9628 W"
                     /collection_date="2015-08-24"
     gene            105..>892
                     /locus_tag="J1Y83_004393"
     CDS             105..>892
                     /locus_tag="J1Y83_004393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011579121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EntS/YbdA MFS transporter"
                     /protein_id="HBA7632459.1"
                     /translation="MQRKTLLSACIALALSGQGWAADITEIETTTGEKKNTNVTCPAD
                     PGKLSPEELKRLPSECSSVVEQNLMPWLVTGAATALITTLAIVELNDDDDHHRNNSPL
                     PPTPPDDDSDDTPVPPTPGGDEIIPDDGPDDTPAPPKPIAFNNDVTLDKTAKTLTIRD
                     SVFSYTENADGTISLQDSNGRKATINLWQIDETNNTVALEGMSADGATKWQYNHNGEL
                     VITGDNTTVNNTGKTIVDGKGATGTEIAGNNAVVNQDGELDVSGG"
BASE COUNT          268 a          204 c          210 g          210 t
ORIGIN      
        1 aggtaacaat ctactggcaa tattggacgt cctctatgtt ttaaataatc aattggtcgg
       61 gtcagggtat ccgaccttct ttatattcgc cagaaggatt tattatgcaa aggaaaactc
      121 tattgtcggc ctgtattgca ttagctctga gtggtcaggg ttgggcggca gatattacag
      181 aaatagaaac caccacaggt gaaaagaaaa ataccaatgt gacttgtcca gcagatcctg
      241 gaaaactcag tccagaagag cttaaacgct taccctctga atgctcctct gtagtcgaac
      301 agaacctgat gccatggctt gtcacaggtg ctgctactgc gttaatcaca accttagcca
      361 tagtggaact aaacgacgat gatgatcatc atcgcaataa ttctccactt ccaccaactc
      421 cacctgatga tgattcagac gacactccag ttcctccaac tcctggcgga gatgagataa
      481 taccggacga cggtccggat gatactcctg cacctcccaa accgattgcg tttaataatg
      541 acgttaccct ggataaaaca gcaaaaacgt taactattcg cgattcagtt ttcagttata
      601 ccgagaatgc tgatgggact atttctctgc aagatagcaa tgggcgtaag gcgactatta
      661 atctttggca gattgatgaa acgaataaca cggttgccct tgaagggatg agcgcagatg
      721 gtgcaacgaa gtggcaatat aatcacaacg gtgagctcgt tattacgggc gacaatacca
      781 cagtaaacaa cactggaaaa accatcgtcg acggcaaagg cgccaccgga actgaaatcg
      841 cgggtaacaa cgcggtggtg aatcaggacg gcgagctgga tgtgagcggc gg
//
LOCUS       DADSYM010000322          891 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2415
            SAMN08039579-rid11723173.denovo.324, whole genome shotgun sequence.
ACCESSION   DADSYM010000322 DADSYM010000000
VERSION     DADSYM010000322.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039579
            Sequence Read Archive: SRR6362533
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 891)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 891)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980162.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 03:39:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,484
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 4,347
            CDSs (with protein)               :: 4,347
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 43 of 85
            Pseudo Genes (incomplete)         :: 37 of 85
            Pseudo Genes (internal stop)      :: 19 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..891
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039579-rid11723173.denovo.324"
                     /strain="LGE2415"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8302 N 95.9628 W"
                     /collection_date="2015-08-24"
     gene            201..803
                     /gene="fimB"
                     /locus_tag="J1Y83_004394"
     CDS             201..803
                     /gene="fimB"
                     /locus_tag="J1Y83_004394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria regulatory protein FimB"
                     /protein_id="HBA7632460.1"
                     /translation="MKNKADNKKRNFLTHSEIESLLKAANTGPHAARNYCLTLLCFIH
                     GFRASEICRLRISDIDLKAKCIYIHRLKKGFSTTHPLLNKEIQALKNWLSIRTSYPHA
                     ESEWVFLSRKGNPLSRQQFYHIISTSGGNAGLSLEIHPHMLRHSCGFALANMGIDTRL
                     IQDYLGHRNIRHTVWYTASNAGRFYGIWDRARGRQRHAVL"
BASE COUNT          267 a          168 c          189 g          267 t
ORIGIN      
        1 catgcaagag catggcgttt gtatggcaaa gttattataa gtaactatcg ctactccatt
       61 taagttcact cagaagaact ggtccactta cgttagttat taagcaaacg ttcgctttta
      121 taaacataat caggattaaa atgttggatt attgctaacc cagcacagct agtgcgcgtc
      181 tgtaattata agggaaaacg atgaagaata aggctgataa caaaaaaagg aacttcctga
      241 cccatagtga aatcgaatca ctccttaaag cagcaaatac cgggcctcat gctgcacgta
      301 attattgtct gacattactt tgttttattc atggtttccg ggcgagtgaa atttgccgat
      361 tgaggatttc ggatattgat cttaaggcaa agtgtatata tatccatcga ttaaaaaaag
      421 gcttttcaac aacgcacccg ctattgaaca aagaaattca ggctttaaaa aactggttga
      481 gtatccgtac ttcttatccg catgctgaga gcgagtgggt atttttatca cgtaagggga
      541 atccactttc tcggcaacag ttttaccata ttatctcgac ttccggtggt aatgccgggt
      601 tgtcactgga gattcacccg cacatgttac gccattcgtg tggttttgct ttggcgaata
      661 tgggaataga tacgcgactt atccaggatt atcttggaca tcgcaatatt cgtcatactg
      721 tctggtatac cgccagcaat gcagggcgtt tttacggcat ctgggataga gccagaggac
      781 gacagcgtca cgctgtttta tagagatagt tgagatacca gggatggtgt taaaaactac
      841 attattctta cgatatatca aaaatgatta accatttatt aattgatgtt a
//
LOCUS       DADSYM010000323          886 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2415
            SAMN08039579-rid11723173.denovo.325, whole genome shotgun sequence.
ACCESSION   DADSYM010000323 DADSYM010000000
VERSION     DADSYM010000323.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039579
            Sequence Read Archive: SRR6362533
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 886)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 886)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980162.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 03:39:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,484
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 4,347
            CDSs (with protein)               :: 4,347
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 43 of 85
            Pseudo Genes (incomplete)         :: 37 of 85
            Pseudo Genes (internal stop)      :: 19 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..886
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039579-rid11723173.denovo.325"
                     /strain="LGE2415"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8302 N 95.9628 W"
                     /collection_date="2015-08-24"
     gene            498..>886
                     /locus_tag="J1Y83_004395"
     CDS             498..>886
                     /locus_tag="J1Y83_004395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber protein"
                     /protein_id="HBA7632461.1"
                     /translation="MASKGRVTALTDNTQGATGLELYEVYNNGYPTAYGNIIHLKGMT
                     AVGEGELLIGWSGTSGAHAPAFIRSRRDTIDANWSPWAQLYTSAHPPAEFYPVGAPIL
                     WPSDTVPSGYALMQGQTFDKSAYPKLAA"
BASE COUNT          244 a          215 c          227 g          200 t
ORIGIN      
        1 cagccaatga caatgctgag aaacgtctgc agaaagatca gaacggtgcg gatatccctg
       61 gcaaagacac ctttacgaaa aatattggtg cctgccgtgc cttcggtggg tcagtaagca
      121 caacaacagg aaactggacg actgcacagt ttatcgagtg gctggattct cagggagcat
      181 ttaaccatcc atactggatg tgcaagggtt cctggtctta tggcaataat aaaatcatta
      241 ctgatactgg ctgcggtaat attcatctcg caggagccgt cattgaagta atggggataa
      301 agtcagcgat gacgatccgc attaccacac cgaccacctc cactggtggc ggaacaacta
      361 acgcccagtt tacctatatt aatcacggaa cagattattc acctggctgg cgaagggact
      421 ataactccag aaataagcca acggcatcag agatcggggc attaccgtca ggtggaacag
      481 cagtatcatc agttaatctg gcttcaaaag gtcgggtaac cgcgctgaca gacaatacgc
      541 agggggcaac aggtcttgag ttatacgagg tgtataacaa cggatatcca acagcgtatg
      601 gaaatatcat tcacctgaaa gggatgacag ccgttggcga aggtgagtta ctcatcggct
      661 ggagtggtac aagcggtgct catgctccgg catttattcg ttcacgacgg gatacgatcg
      721 acgcaaactg gtcgccgtgg gcgcagcttt acacctcggc tcatcctcct gcagagtttt
      781 atccagtcgg tgcaccaatc ctgtggccat cagataccgt tccgtctggt tatgccctga
      841 tgcaggggca gacttttgac aaatctgcat acccgaaact tgcagc
//
LOCUS       DADSYM010000324          875 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2415
            SAMN08039579-rid11723173.denovo.326, whole genome shotgun sequence.
ACCESSION   DADSYM010000324 DADSYM010000000
VERSION     DADSYM010000324.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039579
            Sequence Read Archive: SRR6362533
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 875)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 875)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980162.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 03:39:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,484
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 4,347
            CDSs (with protein)               :: 4,347
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 43 of 85
            Pseudo Genes (incomplete)         :: 37 of 85
            Pseudo Genes (internal stop)      :: 19 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..875
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039579-rid11723173.denovo.326"
                     /strain="LGE2415"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8302 N 95.9628 W"
                     /collection_date="2015-08-24"
     gene            <1..728
                     /gene="ydeO"
                     /locus_tag="J1Y83_004396"
     CDS             <1..728
                     /gene="ydeO"
                     /locus_tag="J1Y83_004396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="acid stress response transcriptional regulator
                     YdeO"
                     /protein_id="HBA7632462.1"
                     /translation="AFNANILDKDYAFSDGEILMVDNAVRTHFEPYERHFKEIGFNEN
                     TIKKYLQCTNIQTVTVPVPAKFLRASNVPTGLLNEMIAYLNSEERNHHNFSELLLFSC
                     LSIFAACKGFITLLTNGVLSVSGKVRNIVNMKLAHPWKLKDICDCLYISESLLKKKLK
                     QEQTTFSQILLDARMQHAKNLIRVEGSVNKIAEQCGYASTSYFIYAFRKHFGNSPKRV
                     SKEYRCQRHTGMNTDNTMSALAI"
BASE COUNT          270 a          170 c          174 g          261 t
ORIGIN      
        1 atgccttcaa cgctaacatt ttagataaag attacgcctt ctctgacggc gagatcctga
       61 tggtagataa cgctgttcgt acgcattttg aaccttatga gcggcatttt aaagaaatcg
      121 gatttaatga aaataccatt aaaaaatatc tacaatgcac taacatccag acagtgacgg
      181 tgcctgttcc tgcgaagttt ttacgtgctt caaatgtacc gactggattg cttaatgaaa
      241 tgattgctta tctcaactcg gaagaacgca atcatcataa tttttcagaa cttttgcttt
      301 tttcttgcct gtctattttt gccgcatgca aaggtttcat tacactatta actaacggtg
      361 tgctatccgt ttccgggaaa gtgagaaata ttgtcaacat gaagctggcg cacccatgga
      421 agctgaaaga tatttgtgac tgcctgtaca ttagtgaaag cctgttgaag aaaaaactaa
      481 agcaagagca aacgacattc tcacagattc ttttagatgc cagaatgcag cacgcaaaaa
      541 atttgatacg cgtagaaggt tcagtcaata aaattgccga acaatgtggt tatgcgagta
      601 catcttattt tatttatgcg ttccgcaaac atttcggcaa cagtccgaag agagtttcta
      661 aggagtaccg ttgtcaaagg cacacgggta tgaacacgga taacacgatg agtgctttag
      721 ctatttgatt atttgctaac gagtagtcaa ccacgcacgt tacgtagaaa ttaaaatggg
      781 gcagccattc cctgccccgc gttgttttta ggcgatatat ttattgaaat aaataagtga
      841 cattcatcac atatttatgc atttgcataa cctgt
//
LOCUS       DADSYM010000325          851 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2415
            SAMN08039579-rid11723173.denovo.327, whole genome shotgun sequence.
ACCESSION   DADSYM010000325 DADSYM010000000
VERSION     DADSYM010000325.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039579
            Sequence Read Archive: SRR6362533
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 851)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 851)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980162.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 03:39:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,484
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 4,347
            CDSs (with protein)               :: 4,347
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 43 of 85
            Pseudo Genes (incomplete)         :: 37 of 85
            Pseudo Genes (internal stop)      :: 19 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..851
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039579-rid11723173.denovo.327"
                     /strain="LGE2415"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8302 N 95.9628 W"
                     /collection_date="2015-08-24"
     gene            16..189
                     /locus_tag="J1Y83_004397"
     CDS             16..189
                     /locus_tag="J1Y83_004397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004700450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EscF/YscF/HrpA family type III secretion system
                     needle major subunit"
                     /protein_id="HBA7632463.1"
                     /translation="MSDSVPELLNSTDLVNDPEKMLELQFAVQQYSAYVNVESGMLKT
                     IKDLVSTISNRSF"
     gene            195..473
                     /gene="escG"
                     /locus_tag="J1Y83_004398"
     CDS             195..473
                     /gene="escG"
                     /locus_tag="J1Y83_004398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE needle protein
                     cochaperone EscG"
                     /protein_id="HBA7632464.1"
                     /translation="MVNDISANKILVWAAVAAANHKLPKYAEAILNVFPQIIPDKKDI
                     AHLEFIILFGLNRKNDAIKALEGCMDDESSQLLYSLVHENVSGWVRGF"
     gene            558..>851
                     /gene="espF"
                     /locus_tag="J1Y83_004399"
     CDS             558..>851
                     /gene="espF"
                     /locus_tag="J1Y83_004399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE effector EspF"
                     /protein_id="HBA7632465.1"
                     /translation="MLNGISNAASTLGRQLVGIASRVSSAGGTGFSVAPQAVRLTPVR
                     VHSPFSPGSSNVNARTIFNMSSQVTSFTPSRPAPPPPTSGQASGASRPLPPIAQ"
BASE COUNT          237 a          152 c          197 g          265 t
ORIGIN      
        1 gaagtaggta aaacgctgag cgattctgtg ccagagttac ttaatagcac cgatttggtt
       61 aatgaccctg aaaaaatgtt agagttgcag tttgcggttc agcaatattc tgcttatgtt
      121 aacgtagaaa gtggaatgtt gaaaacgata aaagatctgg tctcaaccat ttctaaccgt
      181 agtttttaat ttttatggtt aatgatattt ctgctaataa gatactggtg tgggcggctg
      241 tagctgcggc aaaccataag cttcccaaat atgcggaagc tatcctgaat gtattcccac
      301 aaattatacc tgataaaaaa gatatcgcac atttagaatt tattatctta tttggattaa
      361 atagaaaaaa tgatgcgata aaggctttgg agggctgtat ggatgatgag tcgagtcagt
      421 tattgtacag tttggtccat gagaatgtta gtggttgggt acgaggattt taatttattt
      481 tgatttaaaa atcattttat tattcgtttt gatatatatg agagttagcc aagattagat
      541 ataaagaggc ataaattatg cttaatggaa ttagtaacgc tgcttctaca ctagggcggc
      601 agcttgtagg tatcgcaagt cgagtgagct ctgcgggggg aactggattt tctgtagccc
      661 ctcaggctgt tcgtcttact ccggtgagag ttcattcccc cttttctcca ggttcgtcga
      721 atgttaatgc gagaacgatt tttaatatga gcagccaggt gacttcattt actccctctc
      781 gtccggcacc gccgccaccg acaagtggac aggcatcggg ggcatcccga cctttaccgc
      841 ccattgcaca g
//
LOCUS       DADSYM010000326          845 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2415
            SAMN08039579-rid11723173.denovo.328, whole genome shotgun sequence.
ACCESSION   DADSYM010000326 DADSYM010000000
VERSION     DADSYM010000326.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039579
            Sequence Read Archive: SRR6362533
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 845)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 845)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980162.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 03:39:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,484
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 4,347
            CDSs (with protein)               :: 4,347
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 43 of 85
            Pseudo Genes (incomplete)         :: 37 of 85
            Pseudo Genes (internal stop)      :: 19 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..845
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039579-rid11723173.denovo.328"
                     /strain="LGE2415"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8302 N 95.9628 W"
                     /collection_date="2015-08-24"
     gene            complement(<1..>845)
                     /gene="rfaL"
                     /locus_tag="J1Y83_004400"
     CDS             complement(<1..>845)
                     /gene="rfaL"
                     /locus_tag="J1Y83_004400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase RfaL"
                     /protein_id="HBA7632466.1"
                     /translation="IAALLSLVQSPDAGAGMKEIFKAIIENTLLCTIAIPVILRDEKR
                     EDIEKIVFFSFISALGLRCFSELIAYYKDYQQGIMPFADYRHRSISDSMVFLFPALLN
                     LWLIKSAKYRISFAVLSAIFIFLILGTLSRGAWLSVLVIGLIWILMFKQWKLLLVGVM
                     VSIIALSVIFTHKEMTAKLTYKLQQTNSSYRYANGTQGSALDLILENPVIGYGYGNVA
                     YKDVYNKRVIDYPQWTFRQSIGPHNFALFIWFGTGLLGLVSLMMLYCAILKECIKNVV
                     QNKYRS"
BASE COUNT          283 a          164 c          147 g          251 t
ORIGIN      
        1 gagcggtatt tattctggac gacatttttt atacactctt ttagtattgc acagtatagc
       61 atcataagac ttaccagccc taataaacca gtgccaaacc agatgaatag cgcaaaatta
      121 tgtggcccta tcgattgtct aaaggtccat tgcggataat caatgactcg tttattatag
      181 acatctttat atgcaacgtt accgtaaccg taaccaataa caggattttc taatattaga
      241 tcgagtgcgc tgccttgagt accatttgca taacgataag aactattagt ttgttgaagt
      301 ttatacgtta gctttgcggt catctcctta tgtgtgaaaa taaccgacaa tgcaatgatg
      361 gaaaccatta ctcctactaa tagtaacttc cattgtttaa acatcagaat ccatattaat
      421 ccaatgacta acacggaaag ccaggcccct ctggataaag ttcctaatat cagaaaaata
      481 aaaatagcgc ttagaaccgc aaaagaaatg cggtattttg ctgatttgat aagccagaga
      541 tttaacaatg cagggaataa aaaaaccatc gagtcagaaa tgctacggtg tctataatct
      601 gcgaatggca ttatcccttg ttgatagtcc ttataatagg caatcaattc agaaaaacag
      661 cgtaagccta acgcactaat aaatgagaag aaaacgattt tttcgatatc ttctcttttc
      721 tcgtctctca atataaccgg tattgctatt gtgcatagca aagtattttc aatgatagct
      781 ttgaaaattt ccttcatgcc agcacctgca tctggggatt gaacaagtga tagcaatgca
      841 gcaat
//
LOCUS       DADSYM010000327          823 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2415
            SAMN08039579-rid11723173.denovo.329, whole genome shotgun sequence.
ACCESSION   DADSYM010000327 DADSYM010000000
VERSION     DADSYM010000327.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039579
            Sequence Read Archive: SRR6362533
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 823)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 823)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980162.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 03:39:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,484
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 4,347
            CDSs (with protein)               :: 4,347
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 43 of 85
            Pseudo Genes (incomplete)         :: 37 of 85
            Pseudo Genes (internal stop)      :: 19 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..823
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039579-rid11723173.denovo.329"
                     /strain="LGE2415"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8302 N 95.9628 W"
                     /collection_date="2015-08-24"
     gene            <1..>823
                     /locus_tag="J1Y83_004401"
     CDS             <1..>823
                     /locus_tag="J1Y83_004401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020244887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA7632467.1"
                     /translation="EGGVAEKVLSRQEKITPSDENSGYRFLNKSDTYKTILFGQLVLF
                     EKDKSQSLLELSDNVKFYDINSITSNDIIIDGDKRSDKKREFVDSILYFGVLNNHVMI
                     VQSTSLRARELEAHLNWLINTFTDEKESVLMLQDKPSEETMKKMEKAPAKSIKIGGLP
                     ITSEVKNSENQTPSFSNGSVKTIKYRPIGKGGALLKAFFGDNWANDINIKDSLDEANL
                     QVSLEITYFRKTNDSGQAVIDSIATSLRHLDEDDISIKLQGGGEITGKEIKLSGKI"
BASE COUNT          315 a          132 c          163 g          213 t
ORIGIN      
        1 tgagggcggc gttgcagaaa aagtacttag tagacaagaa aaaataacac cttcagatga
       61 aaattcgggg tatcgtttct taaataaaag cgacacatac aaaactatac tttttgggca
      121 actggtttta tttgaaaaag acaaaagcca gtctctatta gagcttagtg ataatgttaa
      181 attttatgac attaactcca tcacgtctaa tgatataatt attgatggag ataaacgtag
      241 tgacaaaaaa agagagtttg tagattcaat tttatatttt ggtgtactaa acaaccatgt
      301 catgatcgtt caatccacat cgcttcgagc aagggagctt gaggcgcatt tgaattggct
      361 aattaataca ttcacagatg aaaaagaatc tgtgttaatg cttcaagata agccatccga
      421 agaaacgatg aaaaaaatgg agaaagcacc agccaagagc ataaaaattg gtggattacc
      481 cattacttca gaagttaaaa attcagagaa ccaaacaccc tccttttcaa atggttcggt
      541 gaagacaata aaataccgcc caatagggaa aggaggtgcg ctactaaaag cattttttgg
      601 cgataactgg gcaaatgata taaacattaa agattcatta gatgaggcca atttacaagt
      661 aagcctcgag attacatact tcagaaaaac aaatgatagc ggtcaagctg ttatagacag
      721 tattgctacc tcactacgac atctagatga ggatgatatt agcatcaaac ttcaaggcgg
      781 tggggaaata acgggaaaag agataaaatt atccggaaaa att
//
LOCUS       DADSYM010000328          821 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2415
            SAMN08039579-rid11723173.denovo.330, whole genome shotgun sequence.
ACCESSION   DADSYM010000328 DADSYM010000000
VERSION     DADSYM010000328.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039579
            Sequence Read Archive: SRR6362533
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 821)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 821)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980162.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 03:39:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,484
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 4,347
            CDSs (with protein)               :: 4,347
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 43 of 85
            Pseudo Genes (incomplete)         :: 37 of 85
            Pseudo Genes (internal stop)      :: 19 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..821
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039579-rid11723173.denovo.330"
                     /strain="LGE2415"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8302 N 95.9628 W"
                     /collection_date="2015-08-24"
     gene            complement(134..697)
                     /locus_tag="J1Y83_004402"
     CDS             complement(134..697)
                     /locus_tag="J1Y83_004402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001561498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HBA7632468.1"
                     /translation="MTRKYLTQDEVYRLMDAAQSMSFPERNRCLIMMAFIHGFRASEL
                     LDLRLSDIDASGKQLNIRRIKNGFSTTHPLLPDEYNLLKLWLKQRKLIENGVEGDWLF
                     LSRKRRPISRQHFFSIIREAGKRAGLAVKAHPHMLRHACGFALADNGVDTRLLQDYLG
                     HRNIQHTVRYTASNAARFKGVWKKKPS"
BASE COUNT          250 a          157 c          165 g          249 t
ORIGIN      
        1 aatcatgggg ttattttact ttagctagaa atatcaacat gttatgtgtt ttatttaatt
       61 gaatgggctg tttttttatg gaagaattga ggaaaacgta ggaatctgac attttgaatc
      121 agaaggtact gttttaacta ggcttttttt tccacacccc tttaaaacga gcagcattac
      181 tggctgtgta tctgacagta tgctgaatat ttcgatgacc cagataatcc tgaagtagtc
      241 gggtatcgac accattgtca gccagagcaa aaccacaggc atgacgtaac atatgaggat
      301 gtgcttttac agctaatcct gcacgttttc cagcctcacg aatgatagaa aaaaaatgtt
      361 gtctgctgat agggcggcgt ttccgggaga gaaaaagcca gtctccttca actccatttt
      421 caattaactt cctctgctta agccacaact tgagcaaatt atattcatca ggaagaagag
      481 gatgtgttgt cgagaagcca tttttaatac gccggatatt aagttgtttt cctgatgcat
      541 caatatcgga taaacgtaaa tcaagaagtt cactggccct aaaaccgtga atgaaagcca
      601 tcataattag acagcgattt ctttcaggaa aggacatgct ctgggctgca tccatcagcc
      661 tgtagacttc atcctgtgtg agatattttc tcgtcatacg ccaccgtgta cagttgtaaa
      721 aaaaagaggc acacccagat aagtgtgcct gcaatcagaa cgttatcgat gtaatttcat
      781 tatctcatta ataagatttt ttgaatagta gttctacgca a
//
LOCUS       DADSYM010000329          801 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2415
            SAMN08039579-rid11723173.denovo.331, whole genome shotgun sequence.
ACCESSION   DADSYM010000329 DADSYM010000000
VERSION     DADSYM010000329.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039579
            Sequence Read Archive: SRR6362533
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 801)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 801)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980162.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 03:39:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,484
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 4,347
            CDSs (with protein)               :: 4,347
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 43 of 85
            Pseudo Genes (incomplete)         :: 37 of 85
            Pseudo Genes (internal stop)      :: 19 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..801
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039579-rid11723173.denovo.331"
                     /strain="LGE2415"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8302 N 95.9628 W"
                     /collection_date="2015-08-24"
     gene            285..740
                     /locus_tag="J1Y83_004403"
     CDS             285..740
                     /locus_tag="J1Y83_004403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001462353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2726 domain-containing protein"
                     /protein_id="HBA7632469.1"
                     /translation="MVAEGILLEGKEWLITRCEKIYFDNLEKWFGHYCHIHCQVSLGR
                     LINFPEQPEFSEEERKRFFAIYNGMAMDYVLVSKKNNKIVGVIELDDPSHERADRIIR
                     DHWLDTIMTLARVPFVRASVTKLEEIPPVWDTRDKIRPLVAATKSEPLH"
BASE COUNT          223 a          157 c          192 g          229 t
ORIGIN      
        1 gcgagtaacg ttggtaatga aatataatca acatggatag gtgaaaatta attatgagct
       61 ttatggcttt tgtaacttgt ttgacaattg catttgtcgc cggttttgtt gttgccagaa
      121 aattgacact ccgaaaagtt gcctcatcgg tgatcatctc taaatcgcaa ccagtccctt
      181 catctgcaac tccccctgct gctaaggcgt cgacaaggag ctatacaccg cagaaaacct
      241 atcgtgattc tcgttggcaa gaacgagatg agttatttca gggaatggtt gccgaaggca
      301 ttttattgga aggaaaagag tggttaatta cgcgttgtga gaaaatctat ttcgataatc
      361 tggagaaatg gtttggacat tattgccata tccattgcca ggtctctctg gggcgcttga
      421 tcaactttcc agaacaacct gagtttagcg aagaagaaag aaagcgtttc tttgctattt
      481 ataatggcat ggcaatggat tatgtgctgg tttcaaagaa gaacaataaa atagtcggtg
      541 ttattgagct ggatgatccg agtcatgaaa gggcagatcg cattattcgt gaccattggt
      601 tggatacgat tatgactctt gctcgggtgc cttttgtacg agcttctgtc actaaattgg
      661 aggaaatccc tccggtctgg gatacccgtg acaaaattcg cccattagtc gcggcgacaa
      721 agagtgaacc gttgcattaa ccaggtatcc ccggtcgggg tttctcatcc ccccggtgta
      781 tgcaatatac gaaaaaaaag c
//
LOCUS       DADSYM010000330          799 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2415
            SAMN08039579-rid11723173.denovo.332, whole genome shotgun sequence.
ACCESSION   DADSYM010000330 DADSYM010000000
VERSION     DADSYM010000330.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039579
            Sequence Read Archive: SRR6362533
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 799)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 799)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980162.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 03:39:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,484
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 4,347
            CDSs (with protein)               :: 4,347
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 43 of 85
            Pseudo Genes (incomplete)         :: 37 of 85
            Pseudo Genes (internal stop)      :: 19 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..799
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039579-rid11723173.denovo.332"
                     /strain="LGE2415"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8302 N 95.9628 W"
                     /collection_date="2015-08-24"
     gene            complement(245..775)
                     /locus_tag="J1Y83_004404"
     CDS             complement(245..775)
                     /locus_tag="J1Y83_004404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001516410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion protein Rhs"
                     /protein_id="HBA7632470.1"
                     /translation="MSLRRMTSGELTLARSIFENTIDYNKVWIHNEKYLPGQGERTAM
                     TPNGEMYYPDAVYSSDFSSQSMPGERETVAGASHLFIHEMMHVWQYQKGYAVKLRGLF
                     SWAANYHYDLNLQSIASYSMEQQASIVADYWLLINYGLVADLANVNYVGDTTESVNTL
                     SEKYKKVLNLFPAGVI"
BASE COUNT          259 a          176 c          119 g          245 t
ORIGIN      
        1 taagtaacat ctgaagatag ctcacttatg tcaatacata cgggttcgaa ctgaccagtc
       61 atttttttca gttttactac attgccatat ttaccttcat ataaaatata atttaaaatc
      121 atactcccat cgtctggttt tgaacaaacc cgtccgtctt gtaaagatat attaccgggc
      181 aacaatgggc gaacataatc accaacagtg catcccgaca aaaagaacaa taaaaaaaat
      241 ctcttcatat gacgcccgca ggaaataaat taagcacttt tttgtatttt tccgatagcg
      301 tgttcactga ttcagttgta tcacctacat agttaacatt tgccaaatca gctaccagtc
      361 cgtagttaat caatagccaa taatcagcta ctatagatgc ttgttgctcc atggaataag
      421 aagcaattga ctgtaaatta agatcgtaat gataatttgc cgcccaggaa aataaaccac
      481 gcaacttaac tgcataccct ttctgatatt gccaaacatg catcatttca tgaataaaca
      541 agtgagaagc acctgctact gtttcacgct caccaggcat tgattgcgat gaaaaatctg
      601 aactataaac agcatcagga taatacattt ccccgtttgg ggtcatcgct gttctttccc
      661 cttgaccggg caaatatttt tcattgtgga tccatacctt gttataatca attgtattct
      721 caaaaatcga tctggctaat gttaattcac cagacgtcat tctccttaat gacatatcct
      781 ttctcctgga aataatatc
//
LOCUS       DADSYM010000331          787 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2415
            SAMN08039579-rid11723173.denovo.333, whole genome shotgun sequence.
ACCESSION   DADSYM010000331 DADSYM010000000
VERSION     DADSYM010000331.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039579
            Sequence Read Archive: SRR6362533
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 787)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 787)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980162.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 03:39:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,484
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 4,347
            CDSs (with protein)               :: 4,347
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 43 of 85
            Pseudo Genes (incomplete)         :: 37 of 85
            Pseudo Genes (internal stop)      :: 19 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..787
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039579-rid11723173.denovo.333"
                     /strain="LGE2415"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8302 N 95.9628 W"
                     /collection_date="2015-08-24"
     gene            20..703
                     /locus_tag="J1Y83_004405"
     CDS             20..703
                     /locus_tag="J1Y83_004405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462932.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA7632471.1"
                     /translation="MEISMKKSLFLLLPLVVTNAHAVYVDVRHEYLDDSKANYDRAYI
                     SHRFANGFGFAIEAISKSGGDDTDRPFNDLEVQGNEYTVSYQFKTGEVAWQPGFVLET
                     GNGYSNYKPYLRATWTLNESWWVGARYRFEYVRRSSDAREDDTVNRMDAWVDYKWNNF
                     DWTIEGIYKKADKYNLYDGGKDNYEYNFRTAYAIGQWTPFVEVGNVSVNSTSDERQTR
                     FRVGLGYTF"
BASE COUNT          223 a          127 c          172 g          265 t
ORIGIN      
        1 gtgtcaatct ttgttttaaa tggaaatttc tatgaaaaaa tctctttttc tgttattgcc
       61 tttagttgtg acaaatgcac atgctgtata tgttgatgtt cggcatgaat accttgatga
      121 tagtaaagct aattatgatc gtgcgtatat ctctcatcgt tttgctaacg gttttggttt
      181 tgctattgag gcaatatcaa aatcgggagg tgatgatact gatagaccat ttaatgatct
      241 tgaagttcag gggaatgaat atactgtcag ctatcagttc aaaacaggag aagttgcctg
      301 gcagccggga tttgtgctgg aaacaggtaa tggttattca aactataaac cttacctccg
      361 agcaacatgg acgttaaatg aaagttggtg ggttggcgct cgctatcgat ttgaatatgt
      421 ccggcgctct tcagatgccc gtgaagatga tactgttaac cgtatggatg cttgggtaga
      481 ctataagtgg aataattttg actggacgat agaaggtatt tataagaaag ccgataaata
      541 taatctatat gatggcggca aagataatta tgagtataac ttcagaactg catacgctat
      601 tgggcagtgg actccatttg ttgaagttgg taacgtttca gttaatagca ccagcgatga
      661 gcgccagact cgcttccgtg tcggtttagg ttataccttc taagcaccta tttgaatatt
      721 tatttcactt ccctttgtta tatcttccaa aagttaattt gaatattgtt taagaattcc
      781 tatttcc
//
LOCUS       DADSYM010000332          783 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2415
            SAMN08039579-rid11723173.denovo.334, whole genome shotgun sequence.
ACCESSION   DADSYM010000332 DADSYM010000000
VERSION     DADSYM010000332.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039579
            Sequence Read Archive: SRR6362533
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 783)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 783)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980162.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 03:39:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,484
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 4,347
            CDSs (with protein)               :: 4,347
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 43 of 85
            Pseudo Genes (incomplete)         :: 37 of 85
            Pseudo Genes (internal stop)      :: 19 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..783
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039579-rid11723173.denovo.334"
                     /strain="LGE2415"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8302 N 95.9628 W"
                     /collection_date="2015-08-24"
     gene            <1..>783
                     /locus_tag="J1Y83_004406"
     CDS             <1..>783
                     /locus_tag="J1Y83_004406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001765455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-specific channel-forming protein Tsx"
                     /protein_id="HBA7632472.1"
                     /translation="NAQEIKIDESWLHQSLNVIGRTDSRFGPRLTNDLYPEYTVAGRK
                     DWFDFYGYVDLPKFFGVGSHYDVGIWDEGSPLFTEIEPRFSIDKLTGLNLAFGPFKEW
                     FIANNYVYDMGDNQSSRQSTWYMGLGTDIDTGLPIKLSANIYAKYQWQNYGAANENEW
                     DGYRFKIKYSIPLTNLFGGRLVYNSFTNFDFGSDLADKSHNNKRTSNAIASSHILSLL
                     YEHWKFAFTLRYFHNGGQWNAGEKVNFGDGSFELKNTGWGTYT"
BASE COUNT          242 a          150 c          166 g          225 t
ORIGIN      
        1 aacgctcagg aaattaaaat tgatgaaagc tggttacatc aaagcttgaa tgtcattggt
       61 cgcacagact ctcgctttgg cccaagactg actaacgacc tctaccctga atatactgta
      121 gcaggaagaa aagactggtt tgatttttat ggttatgttg atctaccgaa attctttggc
      181 gtcggcagtc actatgatgt tgggatctgg gatgaaggct caccactatt tacggaaata
      241 gaacctcggt tttccattga caaattgacc ggattaaatc ttgcgttcgg cccatttaaa
      301 gaatggttca ttgcaaacaa ctatgtctat gatatgggtg acaaccagtc atcccggcaa
      361 agtacatggt atatggggct tggtacagat atcgacacgg gtctaccaat taagctttct
      421 gccaatatat acgccaagta tcagtggcaa aactatggtg ccgctaatga aaatgaatgg
      481 gacggatatc gattcaaaat aaaatatagc atccctctta caaatttatt cggagggcga
      541 ttggtataca atagttttac taactttgat tttggctccg atcttgcgga caagtcacac
      601 aataataaac gaaccagtaa tgctattgct tcaagccata tcctttccct tctatatgaa
      661 cactggaaat ttgcatttac actacgttat tttcacaacg gtggacaatg gaatgcggga
      721 gagaaggtta acttcggaga tggttcattt gaattaaaaa atacaggatg gggaacctat
      781 act
//
LOCUS       DADSYM010000333          750 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2415
            SAMN08039579-rid11723173.denovo.335, whole genome shotgun sequence.
ACCESSION   DADSYM010000333 DADSYM010000000
VERSION     DADSYM010000333.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039579
            Sequence Read Archive: SRR6362533
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 750)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 750)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980162.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 03:39:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,484
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 4,347
            CDSs (with protein)               :: 4,347
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 43 of 85
            Pseudo Genes (incomplete)         :: 37 of 85
            Pseudo Genes (internal stop)      :: 19 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..750
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039579-rid11723173.denovo.335"
                     /strain="LGE2415"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8302 N 95.9628 W"
                     /collection_date="2015-08-24"
     gene            <1..>750
                     /locus_tag="J1Y83_004407"
     CDS             <1..>750
                     /locus_tag="J1Y83_004407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008813516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HBA7632473.1"
                     /translation="FYTAVLKDTCSSALGDIKKQSPKDGRRTPAYYNENELLGWLNVS
                     EIIEIDACDILKKFTYIPLDALFAGNKDLDEQLFNKIVFSSAELKKQDRTIWKIRPAN
                     DKDLQHESLASHYVPYNFSRKYSQKKGEFIIWLSDIHFDGGNGKHAFPTQDNDQLKCL
                     SSRVVELAGKYNNGSKCAGLAISGDLTWQSQKEGFEQALNFIKDVSSSLSLTTDDIII
                     CPGNHDVGLVSKDEYFKIVGKPVTDKPWKILA"
BASE COUNT          242 a          105 c          165 g          238 t
ORIGIN      
        1 ttttataccg ctgtattaaa ggatacttgc tcgtcagctt taggggatat aaaaaagcaa
       61 tctcctaagg atggtcgcag aaccccagca tattataatg agaatgagtt gctgggatgg
      121 ttaaatgttt ctgagattat agaaattgat gcttgtgata tcctcaaaaa attcacatat
      181 attccattgg atgcattgtt tgcaggtaat aaagatttag atgagcaact tttcaacaaa
      241 attgtctttt cttcagctga attaaaaaaa caagatagaa ctatttggaa gattagacct
      301 gcaaatgata aagatttaca gcatgagtca ttagcatctc attatgttcc ttataatttc
      361 agtcggaagt atagccaaaa aaaaggggag tttataattt ggctttcgga tattcatttt
      421 gatggcggaa atgggaaaca tgcatttccg acacaagata atgatcagct aaaatgttta
      481 tcctctcgag tggtggagtt ggctggaaaa tataataatg ggagtaaatg tgctgggttg
      541 gctatttcag gtgatttgac gtggcagtca cagaaagagg gattcgagca agctttgaat
      601 tttatcaaag atgttagttc ttctcttagt ctgacaacgg atgatataat tatttgtcct
      661 gggaatcacg atgtaggtct ggtttctaaa gatgagtact ttaaaatcgt tggtaagcct
      721 gtaacggata aaccatggaa aattctggcg
//
LOCUS       DADSYM010000334          747 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2415
            SAMN08039579-rid11723173.denovo.336, whole genome shotgun sequence.
ACCESSION   DADSYM010000334 DADSYM010000000
VERSION     DADSYM010000334.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039579
            Sequence Read Archive: SRR6362533
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 747)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 747)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980162.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 03:39:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,484
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 4,347
            CDSs (with protein)               :: 4,347
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 43 of 85
            Pseudo Genes (incomplete)         :: 37 of 85
            Pseudo Genes (internal stop)      :: 19 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..747
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039579-rid11723173.denovo.336"
                     /strain="LGE2415"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8302 N 95.9628 W"
                     /collection_date="2015-08-24"
BASE COUNT          251 a          154 c          144 g          198 t
ORIGIN      
        1 aatggtatag aagaaaacta tctcgttaac aaacgacttt aataattgca tttccattta
       61 gttaagcgtt tctctttata agatagatct cattgcataa acaaataaaa cagaataaaa
      121 accaagctga accgtttcac tatactctac ttaattcgag ttacaggaag gcggcaagcg
      181 agtgaatccc caggagctta cataagtaag tgattggggt aagcgaacgc agtcgcagca
      241 catgcaactt gaagtatgac gagtatatat atctattagc tgtctcccgg acataagcta
      301 cagccagcac taacctggaa cgggtgcatt ataaaaagga acaagggaca aatagctgaa
      361 caagtaaccg cgttagtctc ttcccgcata accgtaccat tcgtattaag aagtattgcc
      421 catatccatt cggcgaaaga ggatctgcca tacatcaagt ccgttttact tggcatcaga
      481 tcatcgccgt cattaactct gttaaagtcg gacaaacgat tagcaagatc tgtgattcaa
      541 aagtgcatag cgctaaggtg ggactggtta cagattcatt aaccctgttc tttgtcgcac
      601 ctatcgatcg gtgtaatgcc acaacaggtt tatgttaatc agaacaatgc gcccctctat
      661 aaagagaatt ctttgataag gcaacttgaa aagggaaagc taactgatat caatgtacca
      721 ctttagtaat catcacatta tatacag
//
LOCUS       DADSYM010000335          737 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2415
            SAMN08039579-rid11723173.denovo.337, whole genome shotgun sequence.
ACCESSION   DADSYM010000335 DADSYM010000000
VERSION     DADSYM010000335.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039579
            Sequence Read Archive: SRR6362533
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 737)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 737)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980162.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 03:39:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,484
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 4,347
            CDSs (with protein)               :: 4,347
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 43 of 85
            Pseudo Genes (incomplete)         :: 37 of 85
            Pseudo Genes (internal stop)      :: 19 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..737
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039579-rid11723173.denovo.337"
                     /strain="LGE2415"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8302 N 95.9628 W"
                     /collection_date="2015-08-24"
     gene            22..234
                     /locus_tag="J1Y83_004408"
     CDS             22..234
                     /locus_tag="J1Y83_004408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000608393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA7632474.1"
                     /translation="MINKVILETTTFHDLKLLSECFINIIERAKEENDLATLDDVVDS
                     IATYRIPLQIALTKAREAQQQLEEAA"
     gene            234..542
                     /locus_tag="J1Y83_004409"
     CDS             234..542
                     /locus_tag="J1Y83_004409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000005518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA7632475.1"
                     /translation="MSEVHNVTIQMMDLVIDLQKAVNAAMEYPSELTITQAVINLEST
                     REALQLLIAACEQSEGAQVEADDLSNLIIVINDTTEIMQQAVADLQPLIAELIDKVNQ
                     "
BASE COUNT          257 a          151 c          154 g          175 t
ORIGIN      
        1 ccttaacaaa aggaaaataa aatgattaac aaagtaattt tagagacaac cacttttcat
       61 gatcttaagc ttctttcaga atgttttata aatattattg aacgtgcaaa ggaagagaac
      121 gatctggcaa ctctggatga cgttgtagac agcattgcaa cttacagaat accgctacag
      181 attgcattga ctaaagctcg tgaagcacaa caacaattag aggaagcggc ataatgagcg
      241 aagtacacaa cgtaactatc caaatgatgg accttgttat tgacttgcag aaagcagtta
      301 acgcagcgat ggaatatcca agcgaactga ccatcacaca ggcggttatt aatctggaat
      361 cgacgcggga agcattgcaa ctacttattg cagcgtgcga acaatccgaa ggtgcacagg
      421 ttgaagctga tgatctgagt aacctgatta tcgtgatcaa tgacaccact gaaatcatgc
      481 aacaagcggt agctgatctt caaccactca tagcagaact aatcgataag gttaaccaat
      541 gacagtaaaa accaaacgtg cccgtttact ggcacttaca gaagaaggca tatcgtgcca
      601 ttgtttcaca gtaggcaaga tctacactgg taagctcgac acttacaccc gcctgatgca
      661 cgtaaccgat gataacggta acgaacaaac tattgtttta gatggcaact gcatgttcgg
      721 taagtgggaa ttaacaa
//
LOCUS       DADSYM010000336          734 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2415
            SAMN08039579-rid11723173.denovo.338, whole genome shotgun sequence.
ACCESSION   DADSYM010000336 DADSYM010000000
VERSION     DADSYM010000336.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039579
            Sequence Read Archive: SRR6362533
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 734)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 734)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980162.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 03:39:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,484
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 4,347
            CDSs (with protein)               :: 4,347
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 43 of 85
            Pseudo Genes (incomplete)         :: 37 of 85
            Pseudo Genes (internal stop)      :: 19 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..734
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039579-rid11723173.denovo.338"
                     /strain="LGE2415"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8302 N 95.9628 W"
                     /collection_date="2015-08-24"
     gene            94..>734
                     /locus_tag="J1Y83_004410"
     CDS             94..>734
                     /locus_tag="J1Y83_004410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HBA7632476.1"
                     /translation="MSAGERQEDQAHAALLALETELRTLEKHSGANEKISQQRRDLWK
                     AENQYAVLKEAATKRQLSEQEKSLLTHEKETLEYKRQLAELGDKVEHQKRLNELAQQA
                     ARFEQQQGAKQAAISAQARGLTDRQAQRESEEQRLRDVYGDNPDALAKATSALKNTWS
                     AEEQLRGSWMAGLKSGWGEWAESATDSFSQVKSVATQTFDGIAQNMAAMLTGS"
BASE COUNT          197 a          177 c          248 g          112 t
ORIGIN      
        1 aaaatcctgc aggcggatta caacacgctg atggcggcgg cgaaaaagga ttatgaatcg
       61 acgctgaaaa agccgaagtc gtcaggagtc aaagtgtcag ccggtgagcg tcaggaagac
      121 caggcgcatg ctgccctgct ggcgcttgaa accgagctcc ggacgctgga aaaacacagc
      181 ggtgcgaatg agaaaatcag ccagcagcgt cgcgatttat ggaaagcgga aaatcagtat
      241 gcggtcctga aagaggcagc cacgaaacgg cagttatctg agcaggaaaa atccctgctg
      301 acccatgaga aagagacgct ggagtacaaa cgccagctgg ctgagctggg agacaaagtt
      361 gaacaccaga aacggctgaa tgagctggca cagcaggctg cgcggtttga gcagcagcag
      421 ggcgcgaagc aggcggcaat cagtgcccag gcgcggggcc tcaccgaccg tcaggcgcag
      481 cgggagtcgg aagagcagcg ccttcgtgac gtgtacggtg ataatccgga tgcgctggcg
      541 aaggccacat ctgcactgaa gaacacctgg tctgcggagg agcagcttcg tggaagctgg
      601 atggccggtc tgaagtccgg ctggggcgag tgggcagaaa gtgcgacgga cagtttttcg
      661 caggttaaaa gcgtggccac gcagaccttt gacggtattg cacagaatat ggcagcgatg
      721 ctgaccggca gtga
//
LOCUS       DADSYM010000337          731 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2415
            SAMN08039579-rid11723173.denovo.339, whole genome shotgun sequence.
ACCESSION   DADSYM010000337 DADSYM010000000
VERSION     DADSYM010000337.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039579
            Sequence Read Archive: SRR6362533
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 731)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 731)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980162.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 03:39:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,484
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 4,347
            CDSs (with protein)               :: 4,347
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 43 of 85
            Pseudo Genes (incomplete)         :: 37 of 85
            Pseudo Genes (internal stop)      :: 19 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..731
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039579-rid11723173.denovo.339"
                     /strain="LGE2415"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8302 N 95.9628 W"
                     /collection_date="2015-08-24"
     gene            63..626
                     /locus_tag="J1Y83_004411"
     CDS             63..626
                     /locus_tag="J1Y83_004411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HBA7632477.1"
                     /translation="MKLKHVGMIVVSVLAMSSAAVSAAEGDESVTTTVNGGVIHFKGE
                     VVNAACAIDSESMNQTVELGQVRSSRLAKAGDLSSAVGFNIKLNDCDTNVSSNAAVAF
                     LGTTVTSNDDTLALQSSAAGSAQNVGIQILDRTGEVLVLDGATFSAKTDLIDGTNILP
                     FQARYIALGQSVAGTANADATFKVQYL"
BASE COUNT          189 a          148 c          175 g          219 t
ORIGIN      
        1 acagaatgac ttaattgtta actctcttta ttcagctact taaatataaa ttttggagaa
       61 ttatgaaact caaacatgtc ggtatgattg tcgtttctgt gttggcgatg tcgtctgctg
      121 cggtaagcgc agccgagggt gatgaatcag taacgaccac tgttaatggc ggtgttattc
      181 attttaaagg tgaagtggta aatgccgctt gtgcgattga ttcagaatca atgaaccaaa
      241 cggttgagct gggtcaggtc cgttcttctc gtctggctaa agcgggtgac ctcagctccg
      301 ccgttggctt caatatcaag ctgaatgatt gtgataccaa tgtttccagt aatgcagctg
      361 ttgcattcct gggtactact gtcaccagta atgacgatac attagcgctg cagagttcag
      421 cggcaggctc tgcccaaaat gtcggtatcc aaattttgga ccgtacaggg gaggtattag
      481 tacttgatgg tgctactttt agcgctaaaa ctgacttgat tgatggcacg aatatactgc
      541 cgttccaggc tcgttatatt gctctcgggc agtcagtagc tggtactgca aacgctgatg
      601 cgaccttcaa agttcaatat ctataattca ctcttaaggt gactatgcgt catctgaaat
      661 tgaaacaggg tgtgtgtggc atggctacgc acacccgaga aattcaccaa tgttcatctc
      721 attattaatg a
//
LOCUS       DADSYM010000338          722 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2415
            SAMN08039579-rid11723173.denovo.340, whole genome shotgun sequence.
ACCESSION   DADSYM010000338 DADSYM010000000
VERSION     DADSYM010000338.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039579
            Sequence Read Archive: SRR6362533
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 722)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 722)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980162.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 03:39:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,484
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 4,347
            CDSs (with protein)               :: 4,347
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 43 of 85
            Pseudo Genes (incomplete)         :: 37 of 85
            Pseudo Genes (internal stop)      :: 19 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..722
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039579-rid11723173.denovo.340"
                     /strain="LGE2415"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8302 N 95.9628 W"
                     /collection_date="2015-08-24"
     gene            6..662
                     /locus_tag="J1Y83_004412"
     CDS             6..662
                     /locus_tag="J1Y83_004412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001334609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HBA7632478.1"
                     /translation="MLMSTAVTAAPGDATQFGGADTDWSTVDYPRLTDMDDNVDSMGG
                     KIRFTGRVVKATCKVATDSKQIEVVLPVVPSNLFTGIDVEAQGASNQTDFNINLTECS
                     NTDDQKIEFRFTGTADSANKTLANEVEGSTDADNSGNAGATGVGIRIYSKGTTNNGLI
                     NLNTTAAEGSASTAAYTIPGNATTHDFSAAFTAGYAQNGSTVAPGVVKSTASFVVLYE
                     "
BASE COUNT          207 a          154 c          183 g          178 t
ORIGIN      
        1 caggtatgct gatgtctact gcggtaaccg cagcacccgg cgatgcaaca caatttggtg
       61 gggcggatac tgactggagc accgttgatt atcccaggct cactgatatg gatgacaacg
      121 ttgattcaat gggggggaaa atccgcttta ctggccgtgt agtgaaagct acctgtaagg
      181 tcgcaaccga ttcaaaacag attgaagttg tcctgccggt tgtgccttcc aaccttttca
      241 ctggtatcga cgtagaagca cagggggcga gcaaccagac cgatttcaat attaatctga
      301 ccgaatgtag caatacagat gatcagaaaa ttgagttccg ttttaccggt actgcagata
      361 gcgctaataa aacgctcgct aacgaagtag aaggatcaac ggatgctgac aacagcggca
      421 atgcgggggc gactggtgta gggattcgaa tttactccaa aggtacgacg aataatggtc
      481 tgattaacct gaatactact gcggcagagg gtagcgcctc caccgccgct tatacaattc
      541 caggaaatgc tacgacccat gatttcagcg cggcctttac tgcaggttat gctcaaaacg
      601 gtagcactgt tgcacctggt gtagttaagt caacagcaag ttttgttgtg ctgtacgagt
      661 aaatataaat acgatttcag gttacaaaaa gttgtaacct gagattgaaa ttatttaata
      721 ga
//
LOCUS       DADSYM010000339          713 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2415
            SAMN08039579-rid11723173.denovo.341, whole genome shotgun sequence.
ACCESSION   DADSYM010000339 DADSYM010000000
VERSION     DADSYM010000339.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039579
            Sequence Read Archive: SRR6362533
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 713)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 713)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980162.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 03:39:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,484
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 4,347
            CDSs (with protein)               :: 4,347
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 43 of 85
            Pseudo Genes (incomplete)         :: 37 of 85
            Pseudo Genes (internal stop)      :: 19 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..713
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039579-rid11723173.denovo.341"
                     /strain="LGE2415"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8302 N 95.9628 W"
                     /collection_date="2015-08-24"
     gene            complement(72..683)
                     /locus_tag="J1Y83_004413"
     CDS             complement(72..683)
                     /locus_tag="J1Y83_004413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000492638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector protein Map"
                     /protein_id="HBA7632479.1"
                     /translation="MFSPTAMVGRALAQAVTQTLRPAVTKAATQAGMAASGMRFTPMQ
                     SNLMINHGKLTTQLLQAVAKQTGSSDTQQWFKQEQITFLSRAVNKTVDDYCMSNNSAI
                     SKETKCRIFKEVESAIQQPLDMNCAQSSISHFLQSNKYFNQKVDEQCGKGVDPITRFN
                     TQTKLIEQVSREIFEQNFSTAKISDIKALTEKAIADNVQDTRL"
BASE COUNT          189 a          154 c          137 g          233 t
ORIGIN      
        1 tattttattt taaacgtcta tagagacctt tttgcatcaa ggaaaaaaat aacagcgcag
       61 taagtttccc cctacagccg agtatcctgc acattgtctg caatcgcctt ctcagttaga
      121 gccttgatat cactgatttt cgcggtgcta aaattctgtt caaagatttc gcgtgagact
      181 tgttctatca atttggtttg agtgttaaaa cgtgtaatgg gatccactcc tttaccacac
      241 tgctcatcaa ctttctgatt aaagtattta ttggattgca aaaagtgact aattgatgat
      301 tgtgcacagt tcatatcgag aggctgttgt atcgcgcttt caacctcttt aaaaatgcga
      361 catttagtct ccttactaat tgccgaatta ttactcatgc aatagtcatc cacagttttg
      421 tttactgctc tggatagaaa agtaatctgc tcttgcttga accattgctg ggtatcacta
      481 ctaccagttt gtttggctac ggcctgtagt agctgggtag tcagtttgcc atgattaatc
      541 ataaggttcg actgcatagg tgtgaacctc ataccactgg ctgccatgcc cgcttgcgtc
      601 gcagccttgg ttacagcggg tctaagagtt tgtgtaaccg cctgagctaa cgctctacct
      661 accattgccg ttggactaaa catgctataa acctttataa attacataaa aaa
//
LOCUS       DADSYM010000340          705 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2415
            SAMN08039579-rid11723173.denovo.342, whole genome shotgun sequence.
ACCESSION   DADSYM010000340 DADSYM010000000
VERSION     DADSYM010000340.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039579
            Sequence Read Archive: SRR6362533
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 705)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 705)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980162.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 03:39:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,484
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 4,347
            CDSs (with protein)               :: 4,347
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 43 of 85
            Pseudo Genes (incomplete)         :: 37 of 85
            Pseudo Genes (internal stop)      :: 19 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..705
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039579-rid11723173.denovo.342"
                     /strain="LGE2415"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8302 N 95.9628 W"
                     /collection_date="2015-08-24"
     gene            37..>705
                     /locus_tag="J1Y83_004414"
     CDS             37..>705
                     /locus_tag="J1Y83_004414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBA7632480.1"
                     /translation="MGRKWANIVAKKTAKDGATSKIYAKFGVEIYAAAKQGEPDPELN
                     TSLKFVIERAKQAQVPKHVIDKAIDKAKGGGDETFVQGRYEGFGPNGSMIIAETLTSN
                     VNRTIANVRTIFNKKGGNIGAAGSVSYMFDNTGVIVFKGTDPDHIFEILLEAEVDVRD
                     VTEEEGNIVIYTEPTDLHKGIAALKAAGITEFSTTELEMIAQSEVELSPEDLEIFEGL
                     VDALE"
BASE COUNT          232 a          120 c          158 g          195 t
ORIGIN      
        1 cagtaatgta aataaatttt cgaggagatc attccagtgg gacgtaaatg ggccaatatt
       61 gttgctaaaa aaacggctaa agacggtgca acgtctaaaa tttatgcaaa attcggtgta
      121 gaaatctatg ctgctgccaa acaaggtgaa cccgatccag aattaaacac atctttaaaa
      181 ttcgttattg aacgtgcaaa gcaggcacaa gttccaaagc acgttattga taaagcaatt
      241 gataaagcca aaggcggcgg agatgaaacg ttcgtacagg gacgttatga aggctttggt
      301 cctaatggct caatgattat tgctgagaca ttgacttcaa atgttaaccg tacgattgct
      361 aacgttcgca caattttcaa taaaaaaggc ggcaatatcg gagcggcagg ttctgtcagc
      421 tatatgtttg acaatacggg tgtgattgta tttaaaggaa cagaccctga ccatattttt
      481 gaaattttgc ttgaagctga agttgatgtt cgtgatgtaa ctgaagaaga aggtaatatt
      541 gttatttata ctgaacctac tgaccttcat aaaggaatcg cggcgctaaa agcggctgga
      601 atcactgagt tctcaacaac agaattagaa atgattgctc aatctgaagt tgagctttcc
      661 ccagaagatt tagaaatctt tgaagggctt gttgatgccc ttgaa
//
LOCUS       DADSYM010000341          683 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2415
            SAMN08039579-rid11723173.denovo.343, whole genome shotgun sequence.
ACCESSION   DADSYM010000341 DADSYM010000000
VERSION     DADSYM010000341.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039579
            Sequence Read Archive: SRR6362533
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 683)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 683)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980162.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 03:39:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,484
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 4,347
            CDSs (with protein)               :: 4,347
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 43 of 85
            Pseudo Genes (incomplete)         :: 37 of 85
            Pseudo Genes (internal stop)      :: 19 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..683
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039579-rid11723173.denovo.343"
                     /strain="LGE2415"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8302 N 95.9628 W"
                     /collection_date="2015-08-24"
     gene            <1..186
                     /locus_tag="J1Y83_004415"
     CDS             <1..186
                     /locus_tag="J1Y83_004415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HBA7632481.1"
                     /translation="TTTSTDNGKQNTYFSSLDNMVAQGNVLPVLYGEMRVGSRVISQE
                     ISTADEGDGGEIVVIGR"
     gene            247..>683
                     /locus_tag="J1Y83_004416"
     CDS             247..>683
                     /locus_tag="J1Y83_004416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host specificity protein J"
                     /protein_id="HBA7632482.1"
                     /translation="MGKGSSKGHTPREAKDNLKSTQLLSVIDAISEGPIEGPVDGLKS
                     VLLNSTPVLDSEENTNISGVTVVFRSGEQEQTPPEGFESSGSETVLGTEVKYDTPITR
                     TITSANIDRLRLTFGVQALVETTSKGDRNPSEVRLLVQIQRNGG"
BASE COUNT          167 a          155 c          225 g          136 t
ORIGIN      
        1 acaacgacca gcacggataa cggtaagcag aacacgtatt tctcatcact ggataacatg
       61 gttgcccagg gcaatgttct gcctgttctg tacggtgaaa tgcgtgtggg gtcacgggta
      121 atttcgcagg aaatcagcac ggcagacgaa ggggatggtg gtgaaatcgt ggtgattggt
      181 cgctgatgca gaatgtttta tgtgaaaccg cctccgggcg gttttgtcgt ttatggagcg
      241 tgaggaatgg gtaaaggcag cagtaagggg cataccccgc gcgaagcgaa ggacaacctg
      301 aagtccacgc agctgctgag tgtgatcgat gccatcagcg aagggccgat agaaggtccg
      361 gtggatggat taaaaagcgt gctgctgaac agtacgccgg tgctggacag tgaggagaat
      421 accaatatct ccggcgtcac ggtggtgttc cggtcaggtg agcaggagca gacaccgccg
      481 gaggggtttg aatcctccgg ctccgagacg gtgctgggta cggaagtgaa atatgacacg
      541 ccgataaccc ggaccatcac gtcggcaaac attgaccgtc tgcgcctgac cttcggtgtg
      601 caggcactgg tggaaaccac ctcaaagggg gacaggaatc cgtcggaagt ccgcctgctg
      661 gttcagatac agcgtaatgg tgg
//
LOCUS       DADSYM010000342          675 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2415
            SAMN08039579-rid11723173.denovo.344, whole genome shotgun sequence.
ACCESSION   DADSYM010000342 DADSYM010000000
VERSION     DADSYM010000342.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039579
            Sequence Read Archive: SRR6362533
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 675)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 675)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980162.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 03:39:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,484
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 4,347
            CDSs (with protein)               :: 4,347
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 43 of 85
            Pseudo Genes (incomplete)         :: 37 of 85
            Pseudo Genes (internal stop)      :: 19 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..675
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039579-rid11723173.denovo.344"
                     /strain="LGE2415"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8302 N 95.9628 W"
                     /collection_date="2015-08-24"
     gene            84..>675
                     /gene="fimE"
                     /locus_tag="J1Y83_004417"
     CDS             84..>675
                     /gene="fimE"
                     /locus_tag="J1Y83_004417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria regulatory protein FimE"
                     /protein_id="HBA7632483.1"
                     /translation="MSKRRYLTGKEVQAMMQAVCYGATGARDYCLILLAYRHGMRISE
                     LLDLHYQDLDLNEGRINIRRLKNGFSTVHPLRFDEREAVERWTQERANWKGADRTDAI
                     FISRRGSRLSRQQAYRIIRDAGIEAGTVTQTHPHMLRHACGYELAERGADTRLIQDYL
                     GHRNIRHTVRYTASNAARFAGLWERNNLINEKLKREE"
BASE COUNT          175 a          135 c          172 g          193 t
ORIGIN      
        1 tttatctatt gggctaattt tgaccgattg aggtttccta taggtattca ttcaaatata
       61 tctcagtcag gagtactact attgtgagta aacgtcgtta tcttaccggt aaagaagttc
      121 aggccatgat gcaggcggtg tgttacgggg caacgggagc cagagattat tgtcttattc
      181 tgttggcata tcggcatggg atgcgtatta gtgaactgct tgatctgcat tatcaggatc
      241 ttgaccttaa tgaaggtcga ataaatattc gccgactgaa gaacggattt tctaccgttc
      301 acccgttacg ttttgatgag cgtgaagctg tggaacgctg gacccaggaa cgtgctaact
      361 ggaaaggtgc tgaccggacc gacgctatat ttatttctcg ccgcgggagt cggctttctc
      421 gccagcaggc ctatcgcatt attcgcgatg ccggtattga agctggaacc gtaacgcaga
      481 ctcatcctca tatgttaagg catgcttgcg gttatgaact ggcggagcgt ggtgcagata
      541 cccgtttaat tcaggattat ctcgggcatc gaaatattcg ccatactgtg cgttataccg
      601 ccagtaatgc tgctcgtttt gccggattat gggaaagaaa taatctcata aacgaaaaat
      661 taaaaagaga agaag
//
LOCUS       DADSYM010000343          650 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2415
            SAMN08039579-rid11723173.denovo.345, whole genome shotgun sequence.
ACCESSION   DADSYM010000343 DADSYM010000000
VERSION     DADSYM010000343.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039579
            Sequence Read Archive: SRR6362533
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 650)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 650)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980162.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 03:39:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,484
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 4,347
            CDSs (with protein)               :: 4,347
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 43 of 85
            Pseudo Genes (incomplete)         :: 37 of 85
            Pseudo Genes (internal stop)      :: 19 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..650
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039579-rid11723173.denovo.345"
                     /strain="LGE2415"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8302 N 95.9628 W"
                     /collection_date="2015-08-24"
     gene            99..335
                     /locus_tag="J1Y83_004418"
     CDS             99..335
                     /locus_tag="J1Y83_004418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component-system connector protein YcgZ"
                     /protein_id="HBA7632484.1"
                     /translation="MHQNSVTSDSAGAITRYFAKANLPTQQETLGEIVTEILKDGRNL
                     SRKSLCAKLLCRLEQATGEEEQKHYNALIGLLFE"
     gene            378..650
                     /locus_tag="J1Y83_004419"
     CDS             378..650
                     /locus_tag="J1Y83_004419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component-system connector protein YmgA"
                     /protein_id="HBA7632485.1"
                     /translation="MKTSDNESIKYEITGQAVLQILRMKINFSLQTLIKQLLVMKSAE
                     DNTFRRDLIDSIIRDFSNSDTGGPNRRTAIADNKSMFTGKKINRIH"
BASE COUNT          221 a          111 c          134 g          184 t
ORIGIN      
        1 tgcgaaaaat taatttatca ttctgtacac atatttcgta caagtttgct attgttacct
       61 cacttaacat tgattaacat ttttaacaga ggcgtagcat gcatcaaaat tcagtgactt
      121 cagattctgc aggagcaatc actcgttact ttgcaaaagc taacttgcct actcagcagg
      181 aaactctcgg ggaaattgtg actgagattt tgaaagatgg acgtaatctg agtcgaaagt
      241 cgctttgtgc caaacttctt tgccggctgg aacaagcgac aggggaagag gaacagaaac
      301 attataatgc actaattggg ttgctttttg aataatgcaa cagcctgatt atttaacagg
      361 ttagctatga agtcgttatg aagacatctg ataatgaaag tataaaatat gaaattactg
      421 gccaggcggt gctccagata ctgcgcatga agataaattt ttcgttgcag actcttatta
      481 agcaattact cgtaatgaaa tcggctgagg ataatacttt ccgacgagat ttaattgaca
      541 gcataattcg tgattttagc aacagtgata caggagggcc aaaccgaaga acagcgatag
      601 ctgacaataa aagtatgttc actggtaaga aaataaacag aatacattaa
//
LOCUS       DADSYM010000344          646 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2415
            SAMN08039579-rid11723173.denovo.346, whole genome shotgun sequence.
ACCESSION   DADSYM010000344 DADSYM010000000
VERSION     DADSYM010000344.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039579
            Sequence Read Archive: SRR6362533
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 646)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 646)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980162.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 03:39:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,484
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 4,347
            CDSs (with protein)               :: 4,347
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 43 of 85
            Pseudo Genes (incomplete)         :: 37 of 85
            Pseudo Genes (internal stop)      :: 19 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..646
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039579-rid11723173.denovo.346"
                     /strain="LGE2415"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8302 N 95.9628 W"
                     /collection_date="2015-08-24"
     gene            complement(<1..626)
                     /locus_tag="J1Y83_004420"
     CDS             complement(<1..626)
                     /locus_tag="J1Y83_004420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001305375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3289 family protein"
                     /protein_id="HBA7632486.1"
                     /translation="MPCTLFETITLFDDFSADDMQYGDMEEQDFLSLGLSDISAKVDP
                     YRLIKYHFPGPNSTYGAFSVPTSGTKISQSECIDILFAEMKDLAKMKDLAKMFSFFGQ
                     YKTLIQDLIDHFRYGNGNSFHSQELNLSFHERINKYDYNSPIRVIKECIENDISSTPT
                     IGYRPLLLQKIKTELLSSRLNKFNNFKDNFNGLGISIHDISAQKISLL"
BASE COUNT          210 a           98 c          116 g          222 t
ORIGIN      
        1 tttaaaagag atattttttg agctgagata tcatgaatgc tgattcccag gccattaaaa
       61 ttatctttaa aattattgaa tttatttagt cttgatgaaa gtaattcggt cttaatcttt
      121 tgtaggagaa gaggccggta tcctatcgta ggtgttgaag aaatatcatt ttcgatacat
      181 tctttgatta cacgtattgg gctattataa tcatatttgt ttattcgttc atgaaaagat
      241 aagttcaatt cctggctatg gaaactgttg ccatttccat atctaaaatg atcaatcaga
      301 tcctggatga gtgttttgta ttgtccaaaa aaggaaaaca tttttgccag atctttcatt
      361 tttgccagat ctttcatttc tgcaaataaa atatcaatac attcactttg tgatattttt
      421 gttccagaag ttggaacact aaaagcacca taagtagaat tcggcccagg aaaatggtat
      481 ttaatgaggc ggtaaggatc aacttttgca gagatgtcgc ttaagccgag tgagagaaaa
      541 tcttgttctt ccatatcccc atactgcata tcatcagcag aaaaatcatc gaataaagta
      601 atagtttcga aaagtgtaca gggcatatta attgatgagc ctaata
//
LOCUS       DADSYM010000345          630 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2415
            SAMN08039579-rid11723173.denovo.347, whole genome shotgun sequence.
ACCESSION   DADSYM010000345 DADSYM010000000
VERSION     DADSYM010000345.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039579
            Sequence Read Archive: SRR6362533
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 630)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 630)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980162.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 03:39:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,484
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 4,347
            CDSs (with protein)               :: 4,347
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 43 of 85
            Pseudo Genes (incomplete)         :: 37 of 85
            Pseudo Genes (internal stop)      :: 19 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..630
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039579-rid11723173.denovo.347"
                     /strain="LGE2415"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8302 N 95.9628 W"
                     /collection_date="2015-08-24"
     gene            <1..333
                     /locus_tag="J1Y83_004421"
     CDS             <1..333
                     /locus_tag="J1Y83_004421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA7632487.1"
                     /translation="THSVSGTAASAGNHTHSVTGASAVSQWSQNGSVHKVVSAASVNT
                     SAAGAHTHSVSGTAASAGAHAHTVGIGAHTHSVAIGSHGHTITVNAAGNAENTVKNIA
                     FNYIVRLA"
     gene            333..>630
                     /locus_tag="J1Y83_004422"
     CDS             333..>630
                     /locus_tag="J1Y83_004422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HBA7632488.1"
                     /translation="MAFRMSEQPRTIKIYNLLAGTNEFIGEGDAYIPPHTGLPANSTY
                     IAPPDIPAGFVAVFNSDEASWHLVEDHRGKTVYDVASGDALFISELGSLPENVTW"
BASE COUNT          167 a          153 c          158 g          152 t
ORIGIN      
        1 acgcacagtg tgagcggtac agccgcaagt gccggaaacc atactcatag tgtcacaggc
       61 gcatcagcag tcagccagtg gtcacaaaat gggtcagtac ataaggtagt gtctgcggcc
      121 agtgtgaata caagtgctgc aggagcgcac actcatagtg tcagcggcac agctgcatct
      181 gcaggtgctc acgcacatac tgtcggtatt ggtgctcata cgcactctgt tgcgattggc
      241 tcacatggac acaccatcac cgttaacgct gctggtaacg cggaaaacac cgtcaaaaac
      301 atcgcattta actacattgt gaggcttgca taatggcatt cagaatgagt gaacaaccac
      361 ggaccataaa aatttataat ctgctggccg gaactaatga atttattggt gaaggtgacg
      421 catatattcc gcctcataca ggtctgccag caaacagtac ctatattgca ccgccagata
      481 ttccggctgg cttcgtggct gttttcaaca gtgatgaggc atcgtggcat ctcgttgaag
      541 atcatcgggg taaaacggtt tatgacgtgg cttccggcga cgcgttattt atttctgaac
      601 tcggctcatt accggaaaat gtcacctggt
//
LOCUS       DADSYM010000346          591 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2415
            SAMN08039579-rid11723173.denovo.348, whole genome shotgun sequence.
ACCESSION   DADSYM010000346 DADSYM010000000
VERSION     DADSYM010000346.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039579
            Sequence Read Archive: SRR6362533
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 591)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 591)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980162.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 03:39:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,484
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 4,347
            CDSs (with protein)               :: 4,347
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 43 of 85
            Pseudo Genes (incomplete)         :: 37 of 85
            Pseudo Genes (internal stop)      :: 19 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..591
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039579-rid11723173.denovo.348"
                     /strain="LGE2415"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8302 N 95.9628 W"
                     /collection_date="2015-08-24"
     gene            57..>591
                     /gene="escD"
                     /locus_tag="J1Y83_004423"
     CDS             57..>591
                     /gene="escD"
                     /locus_tag="J1Y83_004423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE inner membrane
                     ring protein EscD"
                     /protein_id="HBA7632489.1"
                     /translation="MLSSYKIKLLNGAMRNRELQLPMGNLTLGTEDNDIVYFPLEQGL
                     NQFLLDIREEGVFLLSPVEFWIDGQPTPYEADKPLPVGKVIDIAGCCFIIGDIDHSLP
                     LSDVPERFSAKNQRKKRLILASVIGATFALSGAIGSYVLLSPKAEPPTFTRADVYQQL
                     KENKLHAITLVWHGKNIA"
BASE COUNT          170 a          124 c          119 g          178 t
ORIGIN      
        1 taagttcacc atattttttc tcattgttca accaccagga tgaataaaat ttaaaaatgt
       61 tatcctcata taaaataaaa cttctcaatg gtgcgatgcg taatcgggaa ttacaacttc
      121 cgatgggaaa cctaacgcta ggtacggaag ataacgatat tgtttacttt ccccttgaac
      181 aaggcctcaa tcagttttta cttgatatac gtgaggaagg tgttttcctt ttatctccgg
      241 ttgaattttg gattgatggg caacccacgc cctatgaagc agataaacca ttacctgtcg
      301 gaaaggttat tgatatagcg ggatgttgct tcatcattgg tgatattgac cattccttgc
      361 cattgtctga tgtgccagaa aggttttctg ccaaaaatca acgcaaaaaa cgccttatcc
      421 tggcgagcgt gattggtgct acttttgcgt tgtctggtgc catcggcagt tacgttttgc
      481 tttccccaaa agctgaaccc ccaacgttta ccagagctga tgtatatcag cagctaaaag
      541 aaaacaaact tcatgcaatc actctggtat ggcatgggaa aaatatcgcg c
//
LOCUS       DADSYM010000347          586 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2415
            SAMN08039579-rid11723173.denovo.349, whole genome shotgun sequence.
ACCESSION   DADSYM010000347 DADSYM010000000
VERSION     DADSYM010000347.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039579
            Sequence Read Archive: SRR6362533
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 586)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 586)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980162.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 03:39:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,484
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 4,347
            CDSs (with protein)               :: 4,347
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 43 of 85
            Pseudo Genes (incomplete)         :: 37 of 85
            Pseudo Genes (internal stop)      :: 19 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..586
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039579-rid11723173.denovo.349"
                     /strain="LGE2415"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8302 N 95.9628 W"
                     /collection_date="2015-08-24"
     gene            1..586
                     /locus_tag="J1Y83_004424"
                     /pseudo
     CDS             1..586
                     /locus_tag="J1Y83_004424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558234.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element IS1397 family transposase"
BASE COUNT          185 a          133 c          157 g          111 t
ORIGIN      
        1 cacaacacgg ttaaaaactg gataaaaggc taccgcaaat ctggtaatga ggcctttatc
       61 aggtgcagga aggagaaaag catgacccgt tctgatgata cccatgaaaa cgaggcaaac
      121 atgacgccgg aagagatgaa gaatgagctg cgttatcttc gcgcagaaaa tgcatatctg
      181 aaagccatgc aggagcatct tctggaaaaa aagcgccagg agctggagaa aaaacgaaag
      241 tcatccagag cctgaggtac ggacactgcc agtctgacct gttaaaggcc gcagggctgg
      301 ccagaagcac gctgtactac cagctgtcac tgcagaaggc taaggacaag tatgctgatg
      361 tgaaacagct catagcctcc atcttccatg aacaccgggg atgttatggg taccggcgga
      421 tccattgtga gcttcagaaa agaggtctta agttcagtgg caaaacggtg cgaaaactga
      481 tgcaacagct cggcctgaag tctcctgtgc gcctgaagaa ataccggtct taccggggaa
      541 atatgggact cgctgcagaa aatatccttc agcgacagtt caaagc
//
LOCUS       DADSYM010000348          575 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2415
            SAMN08039579-rid11723173.denovo.350, whole genome shotgun sequence.
ACCESSION   DADSYM010000348 DADSYM010000000
VERSION     DADSYM010000348.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039579
            Sequence Read Archive: SRR6362533
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 575)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 575)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980162.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 03:39:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,484
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 4,347
            CDSs (with protein)               :: 4,347
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 43 of 85
            Pseudo Genes (incomplete)         :: 37 of 85
            Pseudo Genes (internal stop)      :: 19 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..575
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039579-rid11723173.denovo.350"
                     /strain="LGE2415"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8302 N 95.9628 W"
                     /collection_date="2015-08-24"
     gene            75..>575
                     /locus_tag="J1Y83_004425"
     CDS             75..>575
                     /locus_tag="J1Y83_004425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001445381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS-dependent dihydroxyacetone kinase operon
                     transcriptional regulator DhaR"
                     /protein_id="HBA7632490.1"
                     /translation="MNKESYHNDLKNKWKMFVKHGWVATNSTNHVMLRSWQKCLKHCD
                     PRHWNTPVKASGQTLQTIFSRNEEFIRISQRVVEDHFTLAGDDRLAFLIIDPHGWVLS
                     LNAAGDYSSQLRELGIESGMSWAEDGIGTNVYSLCRETNLYTQLEGAEHFSEQLHCYA
                     MSAAPVI"
BASE COUNT          171 a          102 c          134 g          168 t
ORIGIN      
        1 ttgcgtttga tcaagcttac tggcatagca ttctgataca agttctgaaa atgacttgaa
       61 tggatattat tataatgaat aaagagtcgt atcataatga tctaaaaaat aaatggaaaa
      121 tgtttgttaa gcatggttgg gtcgcgacaa attcgactaa tcatgtgatg ctgaggtcat
      181 ggcaaaaatg tctcaagcat tgtgaccctc gccactggaa cacgcctgtc aaagcttccg
      241 ggcaaacatt gcagacgatt ttttctcgta acgaagagtt tattagaatt tcacaaagag
      301 ttgttgaaga ccatttcacc ctggctggtg acgaccggtt ggcatttttg atcattgacc
      361 cacacggctg ggttctatcg ttgaatgcag caggcgatta ttccagtcaa ttgcgagagt
      421 taggaattga atctggtatg tcatgggccg aagatggcat tggaaccaat gtatatagtc
      481 tttgtcggga aacaaattta tatacacaac tggaaggagc agaacatttt agtgaacagc
      541 tacattgtta tgcgatgagc gctgctccgg tcatt
//
LOCUS       DADSYM010000349          565 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2415
            SAMN08039579-rid11723173.denovo.351, whole genome shotgun sequence.
ACCESSION   DADSYM010000349 DADSYM010000000
VERSION     DADSYM010000349.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039579
            Sequence Read Archive: SRR6362533
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 565)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 565)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980162.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 03:39:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,484
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 4,347
            CDSs (with protein)               :: 4,347
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 43 of 85
            Pseudo Genes (incomplete)         :: 37 of 85
            Pseudo Genes (internal stop)      :: 19 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..565
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039579-rid11723173.denovo.351"
                     /strain="LGE2415"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8302 N 95.9628 W"
                     /collection_date="2015-08-24"
     gene            complement(2..205)
                     /locus_tag="J1Y83_004426"
     CDS             complement(2..205)
                     /locus_tag="J1Y83_004426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001447186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA7632491.1"
                     /translation="MLLIAPGSIAVLITHRFHKALLIAVTISITISGVWLLYFLDRAP
                     APTIVLLFAVAFLFALIIINFRK"
     gene            complement(231..452)
                     /locus_tag="J1Y83_004427"
     CDS             complement(231..452)
                     /locus_tag="J1Y83_004427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001331650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HBA7632492.1"
                     /translation="MPLGIGAFIVGLSCAIATGYLKDNSIIKKDTIIGIVFSGIFVLT
                     INRHKSADYRYHYYITVYSAWYRLPFLPH"
     gene            complement(382..>565)
                     /locus_tag="J1Y83_004428"
     CDS             complement(382..>565)
                     /locus_tag="J1Y83_004428"
                     /inference="COORDINATES: protein motif:HMM:NF013143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HBA7632493.1"
                     /translation="FPCALLSVFLVLKDWALIGDAMNHAMFPDIVLCLDYRYAAGNWC
                     IYCRTVLCNCNWLSER"
BASE COUNT          193 a          105 c          112 g          155 t
ORIGIN      
        1 attatttccg aaaattgatt attataagcg cgaaaagaaa tgctacagca aataagagga
       61 cgattgtcgg cgctggggct ctgtcgagaa aatataataa ccatacacct gatattgtta
      121 ttgatatcgt tacggcgata agtaatgcct tatgaaagcg atgcgtgatg aggacagcaa
      181 tagaccctgg agctattaat agtatataga cagaataatt ccgactgctt ttaatgtggc
      241 aaaaatggta ggcgatacca tgcagagtaa accgtaatgt aataatgata tctgtaatcc
      301 gctgacttgt gcctgttgat agtcaagaca aaaattcctg aaaaaacgat acctataatg
      361 gtatctttct ttatgatgct attatctttc agatagccag ttgcaattgc acaagacagt
      421 cctacaataa atgcaccaat tcccagcggc atacctataa tccaggcata gaactatgtc
      481 aggaaacatt gcgtggttca ttgcatctcc tattagcgcc cagtctttta ataccagaaa
      541 aactgataat aatgcacagg ggaac
//
LOCUS       DADSYM010000350          554 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2415
            SAMN08039579-rid11723173.denovo.352, whole genome shotgun sequence.
ACCESSION   DADSYM010000350 DADSYM010000000
VERSION     DADSYM010000350.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039579
            Sequence Read Archive: SRR6362533
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 554)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 554)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980162.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 03:39:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,484
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 4,347
            CDSs (with protein)               :: 4,347
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 43 of 85
            Pseudo Genes (incomplete)         :: 37 of 85
            Pseudo Genes (internal stop)      :: 19 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..554
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039579-rid11723173.denovo.352"
                     /strain="LGE2415"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8302 N 95.9628 W"
                     /collection_date="2015-08-24"
     gene            complement(<1..>554)
                     /locus_tag="J1Y83_004429"
     CDS             complement(<1..>554)
                     /locus_tag="J1Y83_004429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host specificity protein J"
                     /protein_id="HBA7632494.1"
                     /translation="FMLRLTVAADDGSERLVSTARTAETTYRFTQLAPGNYRLTVRAV
                     NAWGQQGDPASVSFRIAAPAAPSQIELTPGYFQITATPHLAVYDPTVQFEFWFSETRI
                     TDIRQVETTARYLGTALYWIAASINIKPDHDYYFYIRSVNTVGKSAFVEAVGQPSDDA
                     SGYLDFFKGQITESHLGKELLEKV"
BASE COUNT          117 a          161 c          150 g          126 t
ORIGIN      
        1 tcgacttttt ccagcagctc cttgccgaga tgggattcgg ttatctggcc tttgaaaaaa
       61 tccagatagc cggatgcatc atcactcggc tggccaacag cctccacaaa tgccgatttg
      121 ccaacggtgt tcacactgcg gatgtaaaaa taataatcat ggtccggttt gatattgata
      181 ctggcggcta tccagtacag cgccgtgcca agatagcggg ctgtggtttc aacctgcctg
      241 atatcggtaa tccgcgtttc cgagaaccag aactcaaact gtaccgtcgg gtcataaacc
      301 gcaagatgcg gcgtggcggt tatctgaaaa tagcccggcg tcagctcaat ctgtgacggc
      361 gctgccggtg cggcaatcct gaacgatacc gatgccggat cgccctgctg cccccacgca
      421 tttactgccc ggactgtcag cctgtagttc cccggcgcca gttgtgtgaa gcggtatgtg
      481 gtttccgccg tccgggctgt gctgaccagc cgctcactgc cgtcatccgc ggccacggtc
      541 aggcgaagca tgaa
//
LOCUS       DADSYM010000351          539 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2415
            SAMN08039579-rid11723173.denovo.353, whole genome shotgun sequence.
ACCESSION   DADSYM010000351 DADSYM010000000
VERSION     DADSYM010000351.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039579
            Sequence Read Archive: SRR6362533
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 539)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 539)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980162.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 03:39:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,484
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 4,347
            CDSs (with protein)               :: 4,347
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 43 of 85
            Pseudo Genes (incomplete)         :: 37 of 85
            Pseudo Genes (internal stop)      :: 19 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..539
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039579-rid11723173.denovo.353"
                     /strain="LGE2415"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8302 N 95.9628 W"
                     /collection_date="2015-08-24"
     gene            42..>539
                     /locus_tag="J1Y83_004430"
     CDS             42..>539
                     /locus_tag="J1Y83_004430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqaJ viral recombinase family protein"
                     /protein_id="HBA7632495.1"
                     /translation="MTPDIILQRTGIDVRAVEQGDDAWHKLRLGVITASEVHNVIAKP
                     RSGKKWPDMKMSYFHTLLAEVCTGVAPEVNAKALAWGKQYENDARTLFEFTSGVNVTE
                     SPIIYRDESMRTACSPDGLCSDGNGLELKCPFTSRDFMKFRLGGFEAIKSAYMAQVQY
                     SMWVTR"
BASE COUNT          133 a          140 c          153 g          113 t
ORIGIN      
        1 ggattcctga aacagaaagc cactgagcag aaggtggcag catgacaccg gacattatcc
       61 tgcagcgtac cgggatcgac gtgagagctg tcgaacaggg ggatgatgca tggcacaaat
      121 tacggctcgg cgtcatcacc gcttcagaag ttcacaacgt gatagcaaag ccccgctcag
      181 gaaagaagtg gcctgacatg aaaatgtcct acttccacac cctgctggct gaggtttgca
      241 ccggtgtggc tccggaagtt aatgctaagg cgctggcctg gggaaaacag tacgagaacg
      301 acgccagaac cctgtttgaa ttcacttccg gcgtgaatgt tactgaatcc ccgatcatct
      361 atcgcgacga aagtatgcgc accgcctgct ctcccgatgg tttatgcagt gacggcaatg
      421 gccttgagct gaaatgcccg tttacctccc gagatttcat gaagttccgg ctcggtggtt
      481 tcgaggccat aaagtcggct tacatggccc aggtgcagta cagcatgtgg gtgacgcga
//
LOCUS       DADSYM010000352          526 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2415
            SAMN08039579-rid11723173.denovo.354, whole genome shotgun sequence.
ACCESSION   DADSYM010000352 DADSYM010000000
VERSION     DADSYM010000352.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039579
            Sequence Read Archive: SRR6362533
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 526)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 526)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980162.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 03:39:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,484
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 4,347
            CDSs (with protein)               :: 4,347
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 43 of 85
            Pseudo Genes (incomplete)         :: 37 of 85
            Pseudo Genes (internal stop)      :: 19 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..526
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039579-rid11723173.denovo.354"
                     /strain="LGE2415"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8302 N 95.9628 W"
                     /collection_date="2015-08-24"
     gene            complement(<1..525)
                     /locus_tag="J1Y83_004431"
     CDS             complement(<1..525)
                     /locus_tag="J1Y83_004431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001356589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HBA7632496.1"
                     /translation="MDKRPVKLDERETFNVSWGIDENYQVGAAISIASILENNKQNKF
                     TFHIIADYLDKEYIELLSQLATKYQTVIKLYHIDSEPLKELPQSNIWPVSIYYRLLSF
                     DYFSARLDSLLYLDADIVCKGSLNELIALEFKDEYGAVVIDVDAMQSKSAERLCNEDF
                     NGSYFNSGVMYINLR"
BASE COUNT          179 a          106 c           76 g          165 t
ORIGIN      
        1 ccgtaaatta atatacatta caccagagtt aaaatagcta ccgttaaaat cctcattaca
       61 caaacgctca gcacttttac tttgcatggc atctacatca attaccacgg ccccatattc
      121 atctttaaac tctaatgcta ttaactcgtt caatgacccc ttacagacga tatcagcatc
      181 aagatataat aatgaatcca atcgcgcaga aaaataatca aatgaaagca aacgataata
      241 aatagatact ggccagatat ttgattgagg tagctccttc aatggctcag aatcaatatg
      301 atataattta attactgttt gatacttcgt tgctaattgt gataataatt caatatactc
      361 tttgtctagg taatcagcga ttatgtgaaa ggtaaatttg ttttgcttat tattttcaag
      421 aattgaagca attgaaatcg cagccccaac ttggtaattc tcatcaatac cccatgagac
      481 attaaacgtt tcccgttcat caagtttaac ggggcgcttg tccagt
//
LOCUS       DADSYM010000353          516 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2415
            SAMN08039579-rid11723173.denovo.355, whole genome shotgun sequence.
ACCESSION   DADSYM010000353 DADSYM010000000
VERSION     DADSYM010000353.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039579
            Sequence Read Archive: SRR6362533
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 516)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 516)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980162.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 03:39:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,484
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 4,347
            CDSs (with protein)               :: 4,347
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 43 of 85
            Pseudo Genes (incomplete)         :: 37 of 85
            Pseudo Genes (internal stop)      :: 19 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..516
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039579-rid11723173.denovo.355"
                     /strain="LGE2415"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8302 N 95.9628 W"
                     /collection_date="2015-08-24"
     gene            204..>516
                     /locus_tag="J1Y83_004432"
     CDS             204..>516
                     /locus_tag="J1Y83_004432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000513071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA7632497.1"
                     /translation="MGITDKAYADKLRNLETPFLDDRYFFMTINQCDGNGSPMLTDIK
                     VCTTELCGAEYTKKIPTVWLDETLKPTAKRLAIYFIQSPLPYDDKKKQWKVTGWYISS
                     TD"
BASE COUNT          176 a          108 c           80 g          152 t
ORIGIN      
        1 tcactctatt gtattgcgcc agttcatcag caaaaactgg atcccctccc gcaagcacta
       61 taactttcat cgcacaaatt gagcatgttg agctaaataa aacagcatta tctcatgata
      121 tgaaactcat tcttgccgat cgtttttatt tcaagaccag taatccttgt gaaacaaatt
      181 caatttccgc tcgaccagaa tcaatgggca ttactgacaa agcctatgcc gacaaattaa
      241 gaaatttaga aactcctttt cttgatgatc gctatttctt tatgaccatc aatcagtgtg
      301 acggtaatgg tagccccatg ctgacagata taaaagtatg tacaacagaa ttatgtggtg
      361 ctgaatatac aaagaaaata cctactgtat ggcttgatga aacattaaaa ccaactgcta
      421 aacgcctagc aatttatttt attcaatctc ctttacccta tgatgataag aaaaaacaat
      481 ggaaagttac cggatggtat atctcatcaa ctgata
//
LOCUS       DADSYM010000354          515 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2415
            SAMN08039579-rid11723173.denovo.356, whole genome shotgun sequence.
ACCESSION   DADSYM010000354 DADSYM010000000
VERSION     DADSYM010000354.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039579
            Sequence Read Archive: SRR6362533
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 515)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 515)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980162.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 03:39:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,484
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 4,347
            CDSs (with protein)               :: 4,347
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 43 of 85
            Pseudo Genes (incomplete)         :: 37 of 85
            Pseudo Genes (internal stop)      :: 19 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..515
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039579-rid11723173.denovo.356"
                     /strain="LGE2415"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8302 N 95.9628 W"
                     /collection_date="2015-08-24"
     gene            <1..>515
                     /locus_tag="J1Y83_004433"
     CDS             <1..>515
                     /locus_tag="J1Y83_004433"
                     /inference="COORDINATES: protein motif:HMM:NF012744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HBA7632498.1"
                     /translation="QYPKMKIFVVSNCYYGLSTFETKLCFDKAELRLVYLSNLMYSKG
                     IIHLIRAVKRCFKDGMALNLKIAGSISSDEYYSESELKDILFKELQGCDYIQYLGPVR
                     GEEKNNLLLESDVFILPTFYRSEAQPISIIEAMYYGCVIITTGHNYVKEFVGEKNGII
                     IDKNSQQAIVD"
BASE COUNT          168 a           69 c          107 g          171 t
ORIGIN      
        1 acaatatccg aaaatgaaga tttttgtggt ttctaattgc tattatggtc taagtacgtt
       61 tgaaacgaag ttatgctttg ataaagctga gttacgttta gtgtatctgt caaacctaat
      121 gtattctaaa gggatcatac atctaatacg tgctgtgaaa agatgtttta aagatggtat
      181 ggcgcttaat ttaaaaattg cgggttcaat tagctcagac gagtattatt ctgagagtga
      241 attaaaagat attttattta aagagttaca gggttgtgat tatatacaat atctcggtcc
      301 tgtacggggg gaagaaaaaa acaatctcct cttagaatca gatgttttta tattgcctac
      361 cttttatcgt tcagaggccc aaccaatctc catcattgaa gccatgtact atgggtgtgt
      421 aattataacg actgggcata attatgtgaa agagtttgta ggagaaaaaa atggtattat
      481 tatcgataag aatagtcagc aagccatcgt tgatg
//
LOCUS       DADSYM010000355          515 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2415
            SAMN08039579-rid11723173.denovo.357, whole genome shotgun sequence.
ACCESSION   DADSYM010000355 DADSYM010000000
VERSION     DADSYM010000355.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039579
            Sequence Read Archive: SRR6362533
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 515)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 515)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980162.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 03:39:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,484
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 4,347
            CDSs (with protein)               :: 4,347
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 43 of 85
            Pseudo Genes (incomplete)         :: 37 of 85
            Pseudo Genes (internal stop)      :: 19 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..515
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039579-rid11723173.denovo.357"
                     /strain="LGE2415"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8302 N 95.9628 W"
                     /collection_date="2015-08-24"
     gene            <1..>515
                     /locus_tag="J1Y83_004434"
     CDS             <1..>515
                     /locus_tag="J1Y83_004434"
                     /inference="COORDINATES: protein motif:HMM:NF021127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HBA7632499.1"
                     /translation="REKVGAILRHDVSVIENIKKISATDICVANNHYFDFGVNAALNS
                     SKILEDNGFSVHGMFGDSYQEIISADEQIRLAVFNVAESEYGVKTDNSLGFTSITDPN
                     LFSSIANALKNNFIVVFIIHAGLENVLSPLRSWSSIYKTLIDFGVTAIIAHHPHVPQR
                     YERYKNGVIFY"
BASE COUNT          172 a           73 c           94 g          176 t
ORIGIN      
        1 ctagagaaaa agtcggtgca atattacgac atgatgtcag tgttatcgaa aatataaaaa
       61 aaataagtgc aacagatatt tgtgtggcga ataatcacta ttttgatttt ggtgtaaatg
      121 ctgcattaaa tagtagcaaa atacttgagg ataatggatt tagcgttcat gggatgtttg
      181 gagattctta tcaagaaatt atttctgctg atgaacaaat aaggttagcc gtctttaatg
      241 ttgctgaatc tgagtatggc gtaaaaacgg ataactcact tgggtttaca tctattacgg
      301 atcctaattt attttcatca attgctaatg cattaaaaaa taactttata gttgtgttta
      361 taattcatgc aggacttgag aatgttttat cacctttacg ttcatggtca tcaatttaca
      421 aaactttgat agattttggt gttactgcaa ttattgcaca tcacccacat gttccacaac
      481 gatacgaacg ctacaagaat ggagtaatat tttac
//
LOCUS       DADSYM010000356          513 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2415
            SAMN08039579-rid11723173.denovo.358, whole genome shotgun sequence.
ACCESSION   DADSYM010000356 DADSYM010000000
VERSION     DADSYM010000356.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039579
            Sequence Read Archive: SRR6362533
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 513)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 513)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980162.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 03:39:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,484
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 4,347
            CDSs (with protein)               :: 4,347
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 43 of 85
            Pseudo Genes (incomplete)         :: 37 of 85
            Pseudo Genes (internal stop)      :: 19 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..513
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039579-rid11723173.denovo.358"
                     /strain="LGE2415"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8302 N 95.9628 W"
                     /collection_date="2015-08-24"
     gene            complement(<1..494)
                     /locus_tag="J1Y83_004435"
     CDS             complement(<1..494)
                     /locus_tag="J1Y83_004435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001450376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA7632500.1"
                     /translation="MMVHDEWLGDLTGVNQVSFRRLRETSSTRNQLNKILRQEIQDKI
                     SGVELSDINKEGFLYQSIGKIRLLALSSALFDIQCPDYIFSRLYRETLIREIGYQNVK
                     QLSFYWQGGQCKPEYGEERFCAELIKYGAGNLEWLFADNPLWTIVKYLLPKSGEIKPT
                     HIND"
BASE COUNT          165 a          107 c           68 g          173 t
ORIGIN      
        1 aaatcattaa tatgagttgg ttttatttct ccacttttcg gcaaaagata tttgactatg
       61 gtccataaag gattatcagc aaatagccac tccaaatttc ctgcaccgta ttttattaac
      121 tcagcacaaa accgttcttc accatattct ggtttacatt gccctccctg ccaatagaat
      181 gataactgct tcacattttg atatcctatc tctctaatca gagtttctcg atataatcgt
      241 gagaaaatat aatcagggca ttgaatgtca aataatgcac tacttagagc aagcaatctt
      301 attttcccaa ttgactgata taaaaaacct tccttattta tgtcagataa ttccacacca
      361 gaaattttat cttgtatttc ctgacgcaat atcttattga gttgattacg agtagaacta
      421 gtttctctta aacgccgaaa actgacctga ttgacaccgg ttaaatcacc tagccattca
      481 tcatgaacca tcatgattgg ataattaatt aat
//
LOCUS       DADSYM010000357          508 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2415
            SAMN08039579-rid11723173.denovo.359, whole genome shotgun sequence.
ACCESSION   DADSYM010000357 DADSYM010000000
VERSION     DADSYM010000357.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039579
            Sequence Read Archive: SRR6362533
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 508)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 508)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980162.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 03:39:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,484
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 4,347
            CDSs (with protein)               :: 4,347
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 43 of 85
            Pseudo Genes (incomplete)         :: 37 of 85
            Pseudo Genes (internal stop)      :: 19 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..508
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039579-rid11723173.denovo.359"
                     /strain="LGE2415"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8302 N 95.9628 W"
                     /collection_date="2015-08-24"
     gene            127..369
                     /locus_tag="J1Y83_004436"
                     /pseudo
     CDS             127..369
                     /locus_tag="J1Y83_004436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309690.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="omptin family outer membrane protease"
BASE COUNT          175 a          104 c           91 g          138 t
ORIGIN      
        1 aagccattgc gagaccttat gtgtatcagt tttgttcctc ttttttatac aaaaaaacac
       61 agcaattgag gattaacctc atgctattat cgctaatatg catctaaagg catggcactt
      121 gtcagctggg tggaagcatc tggtaacgat aaacactatg acccggggaa aagaatcact
      181 tatcgcagca aggtcaaaga ccaaaattac tattctgttg cagtcaatgc aggttattac
      241 gtaacgccta acgcaaaagt ttatgttgaa ggctcatgga atcgggttac gaataaaaaa
      301 ggtgatactt cactttatga ctacaatgat aacacttctg actacagcaa aaatggtgca
      361 ggcatataaa actataactt catcactact gctggtctta agtacacctt ttaagatcgc
      421 caactaaaat ttccccgagg tgaaaattgc cccagggaat aactagccat ttcaatgtaa
      481 caattaatcc ttaaaataac cccagaag
//
LOCUS       DADSYM010000358          508 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2415
            SAMN08039579-rid11723173.denovo.360, whole genome shotgun sequence.
ACCESSION   DADSYM010000358 DADSYM010000000
VERSION     DADSYM010000358.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039579
            Sequence Read Archive: SRR6362533
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 508)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 508)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980162.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 03:39:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,484
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 4,347
            CDSs (with protein)               :: 4,347
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 43 of 85
            Pseudo Genes (incomplete)         :: 37 of 85
            Pseudo Genes (internal stop)      :: 19 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..508
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039579-rid11723173.denovo.360"
                     /strain="LGE2415"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8302 N 95.9628 W"
                     /collection_date="2015-08-24"
     gene            <1..>508
                     /locus_tag="J1Y83_004437"
     CDS             <1..>508
                     /locus_tag="J1Y83_004437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA7632501.1"
                     /translation="LLKNTEEIQRAVILKMEMMPFLDSVGLVLDDNKYYLFSRRANNK
                     IVVYHQEQVNGPLVDESGRVIFADFNPSKRPWSMASDDSNNSWNPAYNCFDRPGKKCI
                     SFTLHINGKDHDLLAVDKIHVDLNWRYLNEYLDHISANDEVLFLKQGHEIIAKNQLAR
                     EKLIIYNSE"
BASE COUNT          161 a           89 c          109 g          149 t
ORIGIN      
        1 ttattaaaaa atacagagga gattcaacgc gccgtaattc tgaaaatgga aatgatgcca
       61 tttttagact cggtgggact ggtacttgat gataataaat attatctctt ttcgcggagg
      121 gcgaataata aaatcgttgt ttatcatcag gaacaagtaa atggaccgct tgtcgacgag
      181 tcagggcggg ttatttttgc cgattttaac ccatcgaaac gaccgtggtc gatggcttca
      241 gatgactcta acaacagctg gaacccggca tacaattgct ttgatcgtcc gggtaaaaaa
      301 tgtatctctt ttacgctaca catcaacggc aaagatcatg atttgttagc ggtagataaa
      361 attcatgtcg atttaaactg gcgatatctg aacgagtatc ttgatcacat cagcgctaat
      421 gatgaagttc tatttttaaa gcaaggtcat gagatcattg ctaagaatca actcgctcgt
      481 gaaaaattga ttatttataa tagcgaag
//
LOCUS       DADSYM010000359          475 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2415
            SAMN08039579-rid11723173.denovo.361, whole genome shotgun sequence.
ACCESSION   DADSYM010000359 DADSYM010000000
VERSION     DADSYM010000359.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039579
            Sequence Read Archive: SRR6362533
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 475)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 475)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980162.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 03:39:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,484
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 4,347
            CDSs (with protein)               :: 4,347
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 43 of 85
            Pseudo Genes (incomplete)         :: 37 of 85
            Pseudo Genes (internal stop)      :: 19 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..475
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039579-rid11723173.denovo.361"
                     /strain="LGE2415"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8302 N 95.9628 W"
                     /collection_date="2015-08-24"
     gene            complement(145..300)
                     /locus_tag="J1Y83_004438"
     CDS             complement(145..300)
                     /locus_tag="J1Y83_004438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA7632502.1"
                     /translation="MREYPNGEKTHLTVMAAGFPSLTGDHKVIYVAADRHVTSEEILE
                     AAIRLLS"
     gene            complement(380..445)
                     /locus_tag="J1Y83_004439"
     CDS             complement(380..445)
                     /locus_tag="J1Y83_004439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA7632503.1"
                     /translation="MKAPSGAFLLGVYSMDTHILR"
BASE COUNT          156 a           98 c           85 g          136 t
ORIGIN      
        1 ttccagttac acagtaagtc gattatatgg tgcaaacgtg taaaagataa gatgaaacat
       61 cgcaataatc aacatacgat agtctaaatt ttacacaaac agacaaagag aattttcctg
      121 aattatcaat acaatagcat caaatcaact caagagcctt attgctgctt ccagaatttc
      181 ttctgaagta acatgtcgat ccgcggctac ataaatgact ttatgatctc cggtcagaga
      241 tggaaaccct gcggccatta cagtaaggtg tgttttttcg ccatttggat attcacgcat
      301 gatggtgtta actccagtca tcgctggcac taccactgct ggttcagagt taaaaaaaac
      361 tatgattttt ttcatgatgt taccgtagta tgtgagtatc catcgaatag acaccaagca
      421 aaaaagctcc cgaaggagcc ttcattttca cttttttaaa tccaacgaca gacgg
//
LOCUS       DADSYM010000360          463 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2415
            SAMN08039579-rid11723173.denovo.362, whole genome shotgun sequence.
ACCESSION   DADSYM010000360 DADSYM010000000
VERSION     DADSYM010000360.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039579
            Sequence Read Archive: SRR6362533
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 463)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 463)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980162.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 03:39:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,484
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 4,347
            CDSs (with protein)               :: 4,347
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 43 of 85
            Pseudo Genes (incomplete)         :: 37 of 85
            Pseudo Genes (internal stop)      :: 19 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..463
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039579-rid11723173.denovo.362"
                     /strain="LGE2415"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8302 N 95.9628 W"
                     /collection_date="2015-08-24"
BASE COUNT          135 a           98 c           88 g          142 t
ORIGIN      
        1 tctctcatta attgtaagtt cgcaggtatt gtccgatcgt tgaatcattt gggcgttata
       61 acctaaattt ttaccgtcca ataatcaata tttctattgt gattgttaac cactgcgtgc
      121 attgaagggt gaaaaatatg tgcaattgag aagcctttca ttgatgccag ccttgtcatc
      181 atgacaaaca ataatagatg cttattttcc tcgcagggtt ctttcgcact atcaaaaaat
      241 gctcatttac ccggcattac agtcaacgaa gcaacattct atggcctacg ctgatgtaaa
      301 gcacagcgaa aatcctcctt gttgacttcg tctgacctta tcgcggcgac cagcaattat
      361 tcgttttgtg atcctcaaca caaaatgcta ttcgttgcct atgaggggag aagaatttat
      421 ggaaaacgat tgcattcaca aaatgacgtc ctgccagaag aga
//
LOCUS       DADSYM010000361          462 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2415
            SAMN08039579-rid11723173.denovo.363, whole genome shotgun sequence.
ACCESSION   DADSYM010000361 DADSYM010000000
VERSION     DADSYM010000361.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039579
            Sequence Read Archive: SRR6362533
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 462)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 462)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980162.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 03:39:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,484
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 4,347
            CDSs (with protein)               :: 4,347
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 43 of 85
            Pseudo Genes (incomplete)         :: 37 of 85
            Pseudo Genes (internal stop)      :: 19 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..462
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039579-rid11723173.denovo.363"
                     /strain="LGE2415"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8302 N 95.9628 W"
                     /collection_date="2015-08-24"
     gene            <1..>462
                     /locus_tag="J1Y83_004440"
     CDS             <1..>462
                     /locus_tag="J1Y83_004440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA7632504.1"
                     /translation="KYYYIQLRSAFLSAIHHQIFENQLDTETITVSYEFILCQHLITN
                     TEFSYLALPENYNRLFLPNSKNKTNNGLKTLNSEAVGRLLAAGGVYNGNIEGFRDTAE
                     KLGGDAIKGYDQILNEKTAGIAIATASILLTKRSNVDTYTEINSYLGKLRGQ"
BASE COUNT          171 a           84 c           82 g          125 t
ORIGIN      
        1 aagtattatt atatacaact aagaagcgct tttttatcgg ctattcatca ccagatcttt
       61 gaaaaccaac tggatacaga aacaataact gttagttatg aatttatcct ctgccaacat
      121 cttatcacta acaccgagtt cagctatttg gcactccctg aaaattataa ccgcttgttt
      181 ttaccaaatt caaaaaacaa aacaaataat ggtttaaaaa cgttaaacag cgaagctgtt
      241 ggcaggctac ttgctgctgg tggcgtatac aatgggaaca ttgaaggatt cagagatact
      301 gcggaaaaac tgggtggaga tgcaatcaaa ggctatgatc aaatactaaa tgaaaaaaca
      361 gcgggcatag cgatagcaac agcatctatt cttttaacaa agcgttctaa tgttgataca
      421 tatacagaaa taaatagtta cttaggcaaa cttagaggcc aa
//
LOCUS       DADSYM010000362          455 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2415
            SAMN08039579-rid11723173.denovo.364, whole genome shotgun sequence.
ACCESSION   DADSYM010000362 DADSYM010000000
VERSION     DADSYM010000362.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039579
            Sequence Read Archive: SRR6362533
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 455)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 455)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980162.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 03:39:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,484
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 4,347
            CDSs (with protein)               :: 4,347
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 43 of 85
            Pseudo Genes (incomplete)         :: 37 of 85
            Pseudo Genes (internal stop)      :: 19 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..455
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039579-rid11723173.denovo.364"
                     /strain="LGE2415"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8302 N 95.9628 W"
                     /collection_date="2015-08-24"
BASE COUNT          149 a           58 c          106 g          142 t
ORIGIN      
        1 cctatcatat taactagcga gtatttaaca gaggcgagta taatggcatt gatgtttggt
       61 catgccaatg cactatgtat tggggttttt atttctatta tttatataga atgctggagg
      121 taaatagtgt gaacagcatt agtttgatga ttttatactg aactaaaggg ggaaagaggt
      181 aacgaaaaaa gacctccact aggtacgaat ctgaggcggg cagatggagg tcgatatcta
      241 cacgattaaa ttgataagta aaagtcccat cagggattga tctgttcgag tctaatttaa
      301 agtaatttca acgatttggc agaatttctt agagttaatg attttcaatt tttcgatgac
      361 caggaaaaag gctgggtgct gataaaatag tcatctgcat tccttaaatt gtgtaagggt
      421 gaaaggataa agttcaattt aaaggcggtg caata
//
LOCUS       DADSYM010000363          449 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2415
            SAMN08039579-rid11723173.denovo.365, whole genome shotgun sequence.
ACCESSION   DADSYM010000363 DADSYM010000000
VERSION     DADSYM010000363.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039579
            Sequence Read Archive: SRR6362533
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 449)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 449)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980162.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 03:39:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,484
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 4,347
            CDSs (with protein)               :: 4,347
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 43 of 85
            Pseudo Genes (incomplete)         :: 37 of 85
            Pseudo Genes (internal stop)      :: 19 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..449
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039579-rid11723173.denovo.365"
                     /strain="LGE2415"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8302 N 95.9628 W"
                     /collection_date="2015-08-24"
     gene            20..>449
                     /gene="escU"
                     /locus_tag="J1Y83_004441"
     CDS             20..>449
                     /gene="escU"
                     /locus_tag="J1Y83_004441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001412034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE export apparatus
                     switch protein EscU"
                     /protein_id="HBA7632505.1"
                     /translation="MNAYQALVVVAFFVFLLWKGVLFGYLLFSVFDFWFQKHEGLKKM
                     KMSKDEVKREAKDTDGNPEIKGERRRLHSEIQSGSLANNIKKSTVIVKNPTHIAICLY
                     YKLGETPLPLVIETGKDAKALQIIKLAELYDIPVIEDIPLA"
BASE COUNT          141 a           80 c           93 g          135 t
ORIGIN      
        1 gtttgctaat ttcacaggac tgaacgcata tcaagctctt gtcgttgttg ccttttttgt
       61 ttttctttta tggaaaggcg tgctattcgg atatctactc ttttcagtat ttgatttctg
      121 gttccagaag catgagggac tgaagaaaat gaaaatgagt aaagatgagg tgaaacgaga
      181 agccaaggat actgatggta accctgaaat taaaggggag cgccgtcgcc ttcattccga
      241 gatacaaagt ggaagtttgg ctaataacat caaaaaatca accgttattg ttaaaaaccc
      301 gactcacatt gcgatttgcc tatactataa acttggggag actccattac ctttagttat
      361 tgaaacagga aaagatgcca aagctctaca gatcattaaa ctggctgaac tctatgatat
      421 tccagtgatt gaagatattc ctttagcaa
//
LOCUS       DADSYM010000364          437 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2415
            SAMN08039579-rid11723173.denovo.366, whole genome shotgun sequence.
ACCESSION   DADSYM010000364 DADSYM010000000
VERSION     DADSYM010000364.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039579
            Sequence Read Archive: SRR6362533
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 437)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 437)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980162.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 03:39:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,484
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 4,347
            CDSs (with protein)               :: 4,347
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 43 of 85
            Pseudo Genes (incomplete)         :: 37 of 85
            Pseudo Genes (internal stop)      :: 19 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..437
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039579-rid11723173.denovo.366"
                     /strain="LGE2415"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8302 N 95.9628 W"
                     /collection_date="2015-08-24"
BASE COUNT          122 a           82 c           92 g          141 t
ORIGIN      
        1 aaaaatgcat aagtggctgt tctcaaaact tgatgccgga gaattggttg caaaataacc
       61 taccaaaagg agggcgataa tgaaatcgaa catcatccac cttattggta tgttggtctt
      121 ctatgttgct ggttactttt tgttcaagtc agcattgggc tatattacac cgggtgttgt
      181 cctaacagct ccgtgggctg tggttggact catgcaattg ccattatcat attgtataca
      241 agccattttt aaggcaaatg aggccaatga ccactcgtct ttaaccccta gtgaggttag
      301 gcgattagtg ccaatagtca aatccaagag actgaaattg gttttattat tagctttcta
      361 tgtattttca actttgtttg taatcttggg gttaatagca agcacaaatg atcaagccct
      421 actcttcagg gtcttaa
//
LOCUS       DADSYM010000365          433 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2415
            SAMN08039579-rid11723173.denovo.367, whole genome shotgun sequence.
ACCESSION   DADSYM010000365 DADSYM010000000
VERSION     DADSYM010000365.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039579
            Sequence Read Archive: SRR6362533
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 433)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 433)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980162.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 03:39:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,484
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 4,347
            CDSs (with protein)               :: 4,347
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 43 of 85
            Pseudo Genes (incomplete)         :: 37 of 85
            Pseudo Genes (internal stop)      :: 19 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..433
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039579-rid11723173.denovo.367"
                     /strain="LGE2415"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8302 N 95.9628 W"
                     /collection_date="2015-08-24"
BASE COUNT          133 a          100 c           75 g          125 t
ORIGIN      
        1 tacacttaca agacgcgtaa gccccatata taatttttct cgaccgcagg ccaggttcag
       61 acgaagatag ccctccccct ccgttccata gacatgcccc gccataatgc ccgctttgcc
      121 ttcatgaatt aatcgactgt agaattcatc aaaaggataa ccaatagcag aacaatctat
      181 ccaggaaaaa tagcaaccat cagctggcct tacgatcaat tctggaatat tttcattcag
      241 atactgctgg gtgaattgaa tgttagaggc tatatgtgcc tttaatgact ttaaccatcc
      301 ctcacattcg ttgtaggcaa taatagttgc aattatattc agtactgatg gggagtttac
      361 aaaatcgcga tacctcgata tcgttctgaa cttatgatta aaattatcgt cagttgaaat
      421 catatacgat cct
//
LOCUS       DADSYM010000366          431 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2415
            SAMN08039579-rid11723173.denovo.368, whole genome shotgun sequence.
ACCESSION   DADSYM010000366 DADSYM010000000
VERSION     DADSYM010000366.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039579
            Sequence Read Archive: SRR6362533
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 431)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 431)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980162.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 03:39:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,484
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 4,347
            CDSs (with protein)               :: 4,347
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 43 of 85
            Pseudo Genes (incomplete)         :: 37 of 85
            Pseudo Genes (internal stop)      :: 19 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..431
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039579-rid11723173.denovo.368"
                     /strain="LGE2415"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8302 N 95.9628 W"
                     /collection_date="2015-08-24"
BASE COUNT          147 a           73 c           84 g          127 t
ORIGIN      
        1 gatggaacaa ggattttcaa aataaaatat atatggtatt aatatttccc gatctgtatt
       61 caatgattcg ctcaaagatt gaaaatcaag agaaattgtt atagcagtga tacgaaacaa
      121 caaaacggaa tgattgccag ccgttagaaa acactggaca ctctcgtgta aaaagctacc
      181 ctgtctatgg atatagcgat atcgactcat caagcgtgga ctgaatgata ttcagctcca
      241 tatgggcggc aacctggagg ataaacatga tttcgccgtt ggaatcaagg ttgttctgca
      301 atatctacca gactgcttca atttatctgt tttggcagtg ccataatctt aatttaacta
      361 tttgaagatg atgatattgt aagatgccag aaatttagta atctggagaa ccatgtttac
      421 gatcactaaa t
//
LOCUS       DADSYM010000367          429 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2415
            SAMN08039579-rid11723173.denovo.369, whole genome shotgun sequence.
ACCESSION   DADSYM010000367 DADSYM010000000
VERSION     DADSYM010000367.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039579
            Sequence Read Archive: SRR6362533
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 429)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 429)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980162.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 03:39:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,484
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 4,347
            CDSs (with protein)               :: 4,347
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 43 of 85
            Pseudo Genes (incomplete)         :: 37 of 85
            Pseudo Genes (internal stop)      :: 19 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..429
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039579-rid11723173.denovo.369"
                     /strain="LGE2415"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8302 N 95.9628 W"
                     /collection_date="2015-08-24"
     gene            102..293
                     /locus_tag="J1Y83_004442"
     CDS             102..293
                     /locus_tag="J1Y83_004442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2633 family protein"
                     /protein_id="HBA7632506.1"
                     /translation="MSQATSMRKRHRFNSRMTRIVLLISFIFFFGRFIYSSVGAWQHH
                     QSKKEAQQSTLSVESPVQR"
BASE COUNT          104 a          113 c           82 g          130 t
ORIGIN      
        1 cactgacgct tttttacata acgaagaatt gaccattttg tcctgttgtg ccttaatgta
       61 agtaccgtcc acggcgtggg acatacttca aggaaccttt tgtgagtcag gcaaccagta
      121 tgcgaaaacg acaccgattt aacagtcgca tgacccgtat cgtactgctc atcagcttta
      181 tcttcttctt tggccgtttt atctactcgt ccgtcggtgc ctggcagcac catcagagca
      241 aaaaagaagc tcagcaatcc acactctccg tcgaatcacc ggtacaacgt tagcggttac
      301 cttctccact ctcacagaac ataacggcac ctcgctgtcg gatgcttttg ctctttgaga
      361 ttatcaaagc ggcagatatt ctttcatctt aaattttacg tctttgttct gactgaagtt
      421 catcctgtt
//
LOCUS       DADSYM010000368          429 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2415
            SAMN08039579-rid11723173.denovo.370, whole genome shotgun sequence.
ACCESSION   DADSYM010000368 DADSYM010000000
VERSION     DADSYM010000368.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039579
            Sequence Read Archive: SRR6362533
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 429)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 429)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980162.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 03:39:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,484
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 4,347
            CDSs (with protein)               :: 4,347
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 43 of 85
            Pseudo Genes (incomplete)         :: 37 of 85
            Pseudo Genes (internal stop)      :: 19 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..429
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039579-rid11723173.denovo.370"
                     /strain="LGE2415"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8302 N 95.9628 W"
                     /collection_date="2015-08-24"
     gene            complement(<1..>429)
                     /locus_tag="J1Y83_004443"
     CDS             complement(<1..>429)
                     /locus_tag="J1Y83_004443"
                     /inference="COORDINATES: protein motif:HMM:NF025654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="O-antigen polysaccharide polymerase Wzy"
                     /protein_id="HBA7632507.1"
                     /translation="DLRLNETVVRYFSRIVVIVTITGIALTLKDLYNAVSQYGYSALY
                     IIKKENANDSSSMFVTLLLVAIGIVAKENSRSELKILMTVLFFYSFALLLMGARGPMM
                     TYILLWLWLYKKDIKIFKFGLLALLILSLVQFISSLMRTS"
BASE COUNT          155 a           88 c           63 g          123 t
ORIGIN      
        1 ctgatgttcg catcagagaa ctgataaatt gaacaagact taatatcaac aaagccagta
       61 agccgaactt aaagattttg atatctttct tatataacca aagccatagc aatatataag
      121 tcatcattgg tcctctcgcc cccattaata gcaaggcaaa gctatagaag aataaaaccg
      181 tcattagaat ttttaattct gatcgggaat tctccttggc aacaatacca attgctacca
      241 acaataatgt aacaaacatg cttgatgagt cgttagcatt ttccttttta ataatataga
      301 gcgccgagta cccatactgg cttactgcgt tgtacaaatc tttcaaagtt agagcaatac
      361 cggttatagt gacaattact acaatacgag aaaaatatct caccacagtc tcatttaaac
      421 gcaaatcat
//
LOCUS       DADSYM010000369          427 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2415
            SAMN08039579-rid11723173.denovo.371, whole genome shotgun sequence.
ACCESSION   DADSYM010000369 DADSYM010000000
VERSION     DADSYM010000369.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039579
            Sequence Read Archive: SRR6362533
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 427)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 427)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980162.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 03:39:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,484
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 4,347
            CDSs (with protein)               :: 4,347
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 43 of 85
            Pseudo Genes (incomplete)         :: 37 of 85
            Pseudo Genes (internal stop)      :: 19 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..427
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039579-rid11723173.denovo.371"
                     /strain="LGE2415"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8302 N 95.9628 W"
                     /collection_date="2015-08-24"
     gene            231..389
                     /locus_tag="J1Y83_004444"
                     /pseudo
     CDS             231..389
                     /locus_tag="J1Y83_004444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706266.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
BASE COUNT          128 a          108 c           82 g          109 t
ORIGIN      
        1 tggtgggctt ttttatgcaa aacctgaacg tcctgtggta ttcctctggc ataatgattt
       61 tcgcgattaa ccaacaacca gaccagcaaa gacgccacca caagcacagt aacatccatc
      121 aggcttgcca caccccatgc gtgcggctca aagccgccat ggcatattaa tccgcttgcc
      181 attttcgaac agctgattgg tttggtccgg tgtttccctg cttgcactta aaaatgagcg
      241 gataggaaac tctcgaaatg gttcgtcgat atgctcacct cgcacctaat caccttaccg
      301 aacacgcacg gtaaatagat tcgatcctga acccatcagt cccaaatctg tcccaatcaa
      361 aaaataagga aggtattaac gatgtgtaac ctattgattt aaaatggata atataaacag
      421 attgtaa
//
LOCUS       DADSYM010000370          420 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2415
            SAMN08039579-rid11723173.denovo.372, whole genome shotgun sequence.
ACCESSION   DADSYM010000370 DADSYM010000000
VERSION     DADSYM010000370.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039579
            Sequence Read Archive: SRR6362533
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 420)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 420)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980162.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 03:39:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,484
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 4,347
            CDSs (with protein)               :: 4,347
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 43 of 85
            Pseudo Genes (incomplete)         :: 37 of 85
            Pseudo Genes (internal stop)      :: 19 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..420
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039579-rid11723173.denovo.372"
                     /strain="LGE2415"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8302 N 95.9628 W"
                     /collection_date="2015-08-24"
     gene            <1..132
                     /locus_tag="J1Y83_004445"
     CDS             <1..132
                     /locus_tag="J1Y83_004445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease"
                     /protein_id="HBA7632508.1"
                     /translation="LHYVVVERDEKYMAGFDEMVPEFIEKMDEALAEIGFVFGEQWR"
     gene            129..311
                     /locus_tag="J1Y83_004446"
     CDS             129..311
                     /locus_tag="J1Y83_004446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1317 domain-containing protein"
                     /protein_id="HBA7632509.1"
                     /translation="MKHPHDNIRVGTITFVYSVTKRGWVFPGLSVIRNPLKAQRLAEE
                     INNKREAICTKHLLLS"
     gene            284..>420
                     /locus_tag="J1Y83_004447"
     CDS             284..>420
                     /locus_tag="J1Y83_004447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1382 family protein"
                     /protein_id="HBA7632510.1"
                     /translation="MHKASPVELRTSIEMAHSLAQIGVRFVPIPVETDEEFHTLATSL
                     S"
BASE COUNT          114 a           86 c          115 g          105 t
ORIGIN      
        1 ctgcattatg tcgtggttga gcgggatgaa aagtacatgg cgggttttga cgagatggtg
       61 ccggagttca tcgaaaaaat ggacgaggca ctggctgaaa ttggttttgt atttggggag
      121 caatggcgat gaagcatcct cacgataata tccgcgtagg cacgatcact ttcgtctact
      181 ccgttacgaa gcgaggctgg gtatttcccg gcctttctgt tatccgaaat ccgctgaaag
      241 cccagcggct ggctgaggag ataaataata aacgggaggc gatatgcaca aagcatctcc
      301 tgttgagtta agaacgagta ttgagatggc acatagcctt gctcaaattg gagtcaggtt
      361 tgtgccaata ccagtagaaa cagacgaaga atttcatacg ttagccacat ccctttcgca
//
LOCUS       DADSYM010000371          411 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2415
            SAMN08039579-rid11723173.denovo.373, whole genome shotgun sequence.
ACCESSION   DADSYM010000371 DADSYM010000000
VERSION     DADSYM010000371.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039579
            Sequence Read Archive: SRR6362533
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 411)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 411)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980162.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 03:39:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,484
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 4,347
            CDSs (with protein)               :: 4,347
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 43 of 85
            Pseudo Genes (incomplete)         :: 37 of 85
            Pseudo Genes (internal stop)      :: 19 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..411
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039579-rid11723173.denovo.373"
                     /strain="LGE2415"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8302 N 95.9628 W"
                     /collection_date="2015-08-24"
     gene            complement(90..299)
                     /gene="fumD"
                     /locus_tag="J1Y83_004448"
     CDS             complement(90..299)
                     /gene="fumD"
                     /locus_tag="J1Y83_004448"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase FumD"
                     /protein_id="HBA7632511.1"
                     /translation="MGNRTKEDELYREMCRVVGKVVLEMRDLGQEPKHIVIAGVLRTA
                     LANKRIQRSELEKQAMETVINALVK"
BASE COUNT          120 a           99 c           61 g          131 t
ORIGIN      
        1 aattttagca gttgttattt agaatgatta actggaccgc aactgaacta acaatatcag
       61 gatgaagctt accgcctcat cctgaatggt cattttacca gtgcgttaat gactgtttcc
      121 atcgcctgct tttccagctc actgcgctga atgcgtttat tggctaatgc tgtacgtaat
      181 acgcctgcga taacaatatg ctttggttcc tgccccagat cgcgcatttc cagcacaact
      241 ttaccgacaa ctctgcacat ttctcggtac agttcatcct cttttgtccg gtttcccatt
      301 ctttatacct atttatcatg ccaactatca gcatatatca atctaaccaa ttaaacaaaa
      361 atcaaacaaa atcagacaaa taacgtgata aattatttta actgtagcaa t
//
LOCUS       DADSYM010000372          399 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2415
            SAMN08039579-rid11723173.denovo.374, whole genome shotgun sequence.
ACCESSION   DADSYM010000372 DADSYM010000000
VERSION     DADSYM010000372.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039579
            Sequence Read Archive: SRR6362533
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 399)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 399)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980162.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 03:39:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,484
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 4,347
            CDSs (with protein)               :: 4,347
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 43 of 85
            Pseudo Genes (incomplete)         :: 37 of 85
            Pseudo Genes (internal stop)      :: 19 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..399
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039579-rid11723173.denovo.374"
                     /strain="LGE2415"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8302 N 95.9628 W"
                     /collection_date="2015-08-24"
     gene            <1..>399
                     /locus_tag="J1Y83_004449"
     CDS             <1..>399
                     /locus_tag="J1Y83_004449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415890.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA7632512.1"
                     /translation="AGEATEQASAAARSASAAKTSETNAKASETSAESSKTAAASSAS
                     SAASSASSASASKDEATRQASAAKGSATTASTKATEAAGSATAAAQSKSTAESAATRA
                     ETAAKRAEDIASAVALEDASTTKKGIVQLS"
BASE COUNT          109 a          116 c          131 g           43 t
ORIGIN      
        1 aggctggcga agccactgaa caggccagtg cagcagcgag gtctgcttcc gcagcgaaga
       61 catccgagac gaacgcgaaa gcgtcggaaa ccagcgcaga atcctcaaaa acggctgccg
      121 catcgtccgc cagttcggcg gcgtcatcgg cctcatcggc gtctgcttca aaagatgagg
      181 cgaccagaca agcgtccgca gcgaagggca gcgccacgac agcatccacg aaggcgacag
      241 aggcagctgg cagtgcgacg gcggcagcac agagcaaaag tacggcggaa tccgcggcaa
      301 cgcgcgccga gacagcggca aaacgggcag aggatattgc atccgccgtg gcgcttgagg
      361 atgcaagtac gacgaaaaag gggatagtac agctcagca
//
LOCUS       DADSYM010000373          394 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2415
            SAMN08039579-rid11723173.denovo.375, whole genome shotgun sequence.
ACCESSION   DADSYM010000373 DADSYM010000000
VERSION     DADSYM010000373.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039579
            Sequence Read Archive: SRR6362533
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 394)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 394)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980162.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 03:39:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,484
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 4,347
            CDSs (with protein)               :: 4,347
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 43 of 85
            Pseudo Genes (incomplete)         :: 37 of 85
            Pseudo Genes (internal stop)      :: 19 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..394
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039579-rid11723173.denovo.375"
                     /strain="LGE2415"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8302 N 95.9628 W"
                     /collection_date="2015-08-24"
     gene            <1..>394
                     /gene="sepL"
                     /locus_tag="J1Y83_004450"
     CDS             <1..>394
                     /gene="sepL"
                     /locus_tag="J1Y83_004450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type III secretion system LEE gatekeeper SepL"
                     /protein_id="HBA7632513.1"
                     /translation="NMPKGEIVALISSLLQSKFVKIELKKKYARLLLDLLGEDDWELA
                     LLSWLGVGELNQEGIQKIKKLYEKAKDEDSENGASLLDWFMEIKDLPEREKHLKVIIR
                     ALSFDLSYMSSFEDKVRTSSIISDLCRII"
BASE COUNT          119 a           55 c           95 g          125 t
ORIGIN      
        1 caacatgcca aagggggaaa ttgttgcgct aatatcttct ttattgcaat ctaagtttgt
       61 taagattgaa ttaaaaaaga aatatgccag gttattatta gatttattag gcgaagatga
      121 ttgggagctg gctctgcttt cctggttagg ggtgggtgag ttaaatcagg aaggtatcca
      181 gaagatcaag aagctttatg aaaaggctaa ggacgaggac tctgaaaacg gagcctcttt
      241 acttgactgg tttatggaga tcaaggatct tcctgagcgt gaaaagcact taaaggttat
      301 tattagggcg ctatcgttcg atctgtctta tatgtcttct tttgaagaca aggtaagaac
      361 atcttcaatt attagtgatt tatgtcggat aatc
//
LOCUS       DADSYM010000374          372 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2415
            SAMN08039579-rid11723173.denovo.376, whole genome shotgun sequence.
ACCESSION   DADSYM010000374 DADSYM010000000
VERSION     DADSYM010000374.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039579
            Sequence Read Archive: SRR6362533
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 372)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 372)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980162.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 03:39:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,484
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 4,347
            CDSs (with protein)               :: 4,347
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 43 of 85
            Pseudo Genes (incomplete)         :: 37 of 85
            Pseudo Genes (internal stop)      :: 19 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..372
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039579-rid11723173.denovo.376"
                     /strain="LGE2415"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8302 N 95.9628 W"
                     /collection_date="2015-08-24"
     gene            23..>372
                     /gene="ler"
                     /locus_tag="J1Y83_004451"
     CDS             23..>372
                     /gene="ler"
                     /locus_tag="J1Y83_004451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312615.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE master regulator
                     Ler"
                     /protein_id="HBA7632514.1"
                     /translation="MNMENNSHTTSPYIQLIEQIAVLQQEAKRLREQEVQSVIESIQK
                     QITYYNITLQELGYTNVPDDGLARRNSSKGVYYRNEEGQTWSGVGRQPRWLKEALLNG
                     MKKEDFLVKDTEEE"
BASE COUNT          130 a           64 c           92 g           86 t
ORIGIN      
        1 aagcatgcgg agattattta ttatgaatat ggaaaataat tcacatacaa caagtccata
       61 cattcagctt atagagcaga ttgcagttct acagcaggaa gcaaagcgac tgcgagagca
      121 ggaagttcaa agtgtaattg agtcgattca gaagcagatt acttattaca atataacctt
      181 acaagagctg ggatatacta atgtgcctga tgatggactc gctcgccgga actcatcgaa
      241 aggtgtttac taccgaaatg aagaagggca gacctggtcg ggcgtaggcc gacagccacg
      301 ctggcttaaa gaagcactgt tgaatggaat gaagaaagaa gattttcttg tgaaggacac
      361 tgaagaagaa at
//
LOCUS       DADSYM010000375          360 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2415
            SAMN08039579-rid11723173.denovo.377, whole genome shotgun sequence.
ACCESSION   DADSYM010000375 DADSYM010000000
VERSION     DADSYM010000375.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039579
            Sequence Read Archive: SRR6362533
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 360)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 360)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980162.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 03:39:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,484
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 4,347
            CDSs (with protein)               :: 4,347
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 43 of 85
            Pseudo Genes (incomplete)         :: 37 of 85
            Pseudo Genes (internal stop)      :: 19 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..360
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039579-rid11723173.denovo.377"
                     /strain="LGE2415"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8302 N 95.9628 W"
                     /collection_date="2015-08-24"
BASE COUNT           91 a           96 c           78 g           95 t
ORIGIN      
        1 ggtttgggta ggtagcccca gggttgatag aaatatcgtc ctggggcttc atctttccag
       61 atctcagagt aaacctgaaa ccggaaagct tcaggcaccc gcagttatct tacccttcct
      121 tagcaaaata gctgtttatt tatacagtga gtcctgttta ggacttttca cctgtattat
      181 cgcccatgcc agtaaaacga aatcgcccgc agccatctct gactgcgggc gaaagtgtaa
      241 agcaggtgcc ttaccatcct gacctgacaa ccggatatgc ggggattact ccccgcacaa
      301 cggctacttc ttcggttcgt aagcgagaac agccttaaac tcaatattac gttccttcac
//
LOCUS       DADSYM010000376          354 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2415
            SAMN08039579-rid11723173.denovo.379, whole genome shotgun sequence.
ACCESSION   DADSYM010000376 DADSYM010000000
VERSION     DADSYM010000376.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039579
            Sequence Read Archive: SRR6362533
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 354)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 354)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980162.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 03:39:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,484
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 4,347
            CDSs (with protein)               :: 4,347
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 43 of 85
            Pseudo Genes (incomplete)         :: 37 of 85
            Pseudo Genes (internal stop)      :: 19 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..354
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039579-rid11723173.denovo.379"
                     /strain="LGE2415"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8302 N 95.9628 W"
                     /collection_date="2015-08-24"
     gene            <1..>354
                     /locus_tag="J1Y83_004452"
     CDS             <1..>354
                     /locus_tag="J1Y83_004452"
                     /inference="COORDINATES: protein motif:HMM:NF025072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HBA7632515.1"
                     /translation="SDFRHYFCEQTRMVFVGRLTKKSNLDFLINSMKYLPGNFQLAVI
                     GDGPLKEKLIGLANELNVSERIVWHGAIYDEKRIASVMLKSDVFVYPGSVGLSLIHAY
                     NYGLPSIIHNNRDMH"
BASE COUNT          103 a           55 c           70 g          126 t
ORIGIN      
        1 gagtgatttc cgacattatt tttgtgagca gacaagaatg gttttcgtgg gacgattgac
       61 aaaaaaatct aatttggatt ttcttattaa ctctatgaaa taccttcctg ggaattttca
      121 gcttgcagtt attggtgatg gtccgttaaa agaaaagctt attggccttg ctaatgaact
      181 taatgtatcc gaacgaattg tatggcatgg cgctatttat gatgaaaaac gcatcgcttc
      241 agtgatgctg aaatctgatg tttttgtata tcctggttcc gttggattaa gtttaattca
      301 cgcatataat tatggactac cttcaattat tcataataat cgcgatatgc acat
//
LOCUS       DADSYM010000377          331 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2415
            SAMN08039579-rid11723173.denovo.381, whole genome shotgun sequence.
ACCESSION   DADSYM010000377 DADSYM010000000
VERSION     DADSYM010000377.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039579
            Sequence Read Archive: SRR6362533
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 331)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 331)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980162.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 03:39:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,484
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 4,347
            CDSs (with protein)               :: 4,347
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 43 of 85
            Pseudo Genes (incomplete)         :: 37 of 85
            Pseudo Genes (internal stop)      :: 19 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..331
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039579-rid11723173.denovo.381"
                     /strain="LGE2415"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8302 N 95.9628 W"
                     /collection_date="2015-08-24"
     gene            24..100
                     /locus_tag="J1Y83_004453"
     tRNA            24..100
                     /locus_tag="J1Y83_004453"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:58..60,aa:Ile,seq:gat)
     gene            143..218
                     /locus_tag="J1Y83_004454"
     tRNA            143..218
                     /locus_tag="J1Y83_004454"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:176..178,aa:Ala,seq:tgc)
BASE COUNT           96 a           72 c           80 g           83 t
ORIGIN      
        1 aaaatgagca gtaaaacctc tacaggcttg tagctcaggt ggttagagcg cacccctgat
       61 aagggtgagg tcggtggttc aagtccactc aggcctacca aatttgcacc gcaaatttga
      121 agaggtttta actacatgtt atggggctat agctcagctg ggagagcgcc tgctttgcac
      181 gcaggaggtc tgcggttcga tcccgcatag ctccaccatc tctgtagtga ttaaataaaa
      241 aatacttcag agtgtacctg caaaggttca ctgcgaagtt ttgctcttta aaaatctgga
      301 tcaagctgaa aattgaaaca ctgaacaacg a
//
LOCUS       DADSYM010000378          327 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2415
            SAMN08039579-rid11723173.denovo.382, whole genome shotgun sequence.
ACCESSION   DADSYM010000378 DADSYM010000000
VERSION     DADSYM010000378.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039579
            Sequence Read Archive: SRR6362533
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 327)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 327)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980162.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 03:39:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,484
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 4,347
            CDSs (with protein)               :: 4,347
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 43 of 85
            Pseudo Genes (incomplete)         :: 37 of 85
            Pseudo Genes (internal stop)      :: 19 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..327
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039579-rid11723173.denovo.382"
                     /strain="LGE2415"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8302 N 95.9628 W"
                     /collection_date="2015-08-24"
     gene            117..>327
                     /gene="grlR"
                     /locus_tag="J1Y83_004455"
     CDS             117..>327
                     /gene="grlR"
                     /locus_tag="J1Y83_004455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE GrlA-binding
                     negative regulator GrlR"
                     /protein_id="HBA7632516.1"
                     /translation="MIMKDGIYSIIFISNEDSCGEGILIKNGNMITGGDIASVYQGVL
                     SEDEDIILHVHRYNYEIPSVLNIEQD"
BASE COUNT          108 a           43 c           73 g          103 t
ORIGIN      
        1 tttaatgata tattacattg caatctggag aaaaagaaag gtctccatta ttcttgatat
       61 tgcttatgga tagaacaaat tgaaaggagt gaggttagta tgaaactgag tgagttatga
      121 ttatgaagga tggcatctat agcattatat ttattagcaa tgaagactcc tgtggggaag
      181 gtatactgat taaaaatgga aatatgatca ctggcggcga tattgcttct gtgtatcagg
      241 gggttctctc tgaagatgag gacatcatac ttcatgtcca tcgatataat tacgaaattc
      301 cctcggtgct aaacattgaa caagatt
//
LOCUS       DADSYM010000379          322 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2415
            SAMN08039579-rid11723173.denovo.383, whole genome shotgun sequence.
ACCESSION   DADSYM010000379 DADSYM010000000
VERSION     DADSYM010000379.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039579
            Sequence Read Archive: SRR6362533
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 322)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 322)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980162.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 03:39:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,484
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 4,347
            CDSs (with protein)               :: 4,347
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 43 of 85
            Pseudo Genes (incomplete)         :: 37 of 85
            Pseudo Genes (internal stop)      :: 19 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..322
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039579-rid11723173.denovo.383"
                     /strain="LGE2415"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8302 N 95.9628 W"
                     /collection_date="2015-08-24"
BASE COUNT           69 a           83 c           87 g           83 t
ORIGIN      
        1 taacggtgat ggtgtgtcca tgtgaaccaa tcgcaacaga gtgcgtatga gcaccaatac
       61 cgacagtatg cgcgtgtgca cccgcagatg ctgctgtgcc ggacagtgag tgtgtatggt
      121 ttccttctga agatgattta cctacccatt gggaggtgcc actggttaat gagccactga
      181 taccaacctg attagtcccg ctaacttgag tactccctgc cggaaacatt ccagtaggtt
      241 gactcccgct attcgttctg ggcccagtct gactatgttg gtgggcccca gctgaacttg
      301 tagagccgct cagactgtgc gt
//
LOCUS       DADSYM010000380          310 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2415
            SAMN08039579-rid11723173.denovo.384, whole genome shotgun sequence.
ACCESSION   DADSYM010000380 DADSYM010000000
VERSION     DADSYM010000380.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039579
            Sequence Read Archive: SRR6362533
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 310)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 310)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980162.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 03:39:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,484
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 4,347
            CDSs (with protein)               :: 4,347
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 43 of 85
            Pseudo Genes (incomplete)         :: 37 of 85
            Pseudo Genes (internal stop)      :: 19 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..310
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039579-rid11723173.denovo.384"
                     /strain="LGE2415"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8302 N 95.9628 W"
                     /collection_date="2015-08-24"
     gene            <1..>310
                     /gene="cesT"
                     /locus_tag="J1Y83_004456"
     CDS             <1..>310
                     /gene="cesT"
                     /locus_tag="J1Y83_004456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type III secretion system LEE chaperone CesT"
                     /protein_id="HBA7632517.1"
                     /translation="DEYMMIYGVCGKFPTDNPNFALEILNANLWFAENGGPYLCYESG
                     AQSLLLALRFPLDDATPEKLENEIEVVVKSMENLYLVLHNQGITLENEHMKIEEISSS
                     "
BASE COUNT          101 a           51 c           71 g           87 t
ORIGIN      
        1 atgacgaata tatgatgatt tatggcgtct gtggaaaatt cccgacagat aaccctaact
       61 tcgctcttga gattttgaat gcaaacttat ggtttgcaga gaatggtggg ccatacctgt
      121 gttatgagtc aggagcacaa tcgctgttgt tagcgttacg tttcccgctc gatgatgcta
      181 cccctgaaaa acttgagaat gaaatagaag tcgtcgttaa gtcgatggaa aatctgtatt
      241 tggtattaca taatcaggga ataacattag aaaacgaaca tatgaaaata gaggaaatta
      301 gctcaagcga
//
LOCUS       DADSYM010000381          307 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2415
            SAMN08039579-rid11723173.denovo.385, whole genome shotgun sequence.
ACCESSION   DADSYM010000381 DADSYM010000000
VERSION     DADSYM010000381.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039579
            Sequence Read Archive: SRR6362533
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 307)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 307)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980162.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 03:39:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,484
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 4,347
            CDSs (with protein)               :: 4,347
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 43 of 85
            Pseudo Genes (incomplete)         :: 37 of 85
            Pseudo Genes (internal stop)      :: 19 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..307
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039579-rid11723173.denovo.385"
                     /strain="LGE2415"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8302 N 95.9628 W"
                     /collection_date="2015-08-24"
     gene            <1..180
                     /gene="cspI"
                     /locus_tag="J1Y83_004457"
     CDS             <1..180
                     /gene="cspI"
                     /locus_tag="J1Y83_004457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001179150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock protein CspI"
                     /protein_id="HBA7632518.1"
                     /translation="FNPEKGFGFITPKDGSKDVFVHFSAIQSNDFKTLTENQEVEFGI
                     ENGPKGPAAVHVVAL"
     gene            191..>307
                     /locus_tag="J1Y83_004458"
     CDS             191..>307
                     /locus_tag="J1Y83_004458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HBA7632519.1"
                     /translation="MTNHIHFRCPCCHGSQYRTSSFDVSDMNPFGAKCIFCKS"
BASE COUNT           89 a           57 c           64 g           97 t
ORIGIN      
        1 tttaaccctg aaaaaggttt tggtttcatc acgccgaaag atggcagcaa agatgtgttt
       61 gtccatttct cagcaattca gagcaacgat ttcaaaacat taactgagaa tcaggaagtt
      121 gaatttggta ttgagaacgg acctaaaggt cctgccgctg ttcatgtagt ggcgctttga
      181 ggtagacaat attacaaacc atattcactt tagatgcccg tgttgtcatg gttcccagta
      241 tagaacatca tcttttgatg tttctgacat gaatcctttc ggggcaaaat gtatcttttg
      301 taaatca
//
LOCUS       DADSYM010000382        15899 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2415
            SAMN08039579-rid11723173.guided.113, whole genome shotgun sequence.
ACCESSION   DADSYM010000382 DADSYM010000000
VERSION     DADSYM010000382.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039579
            Sequence Read Archive: SRR6362533
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15899)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15899)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980162.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 03:39:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,484
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 4,347
            CDSs (with protein)               :: 4,347
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 43 of 85
            Pseudo Genes (incomplete)         :: 37 of 85
            Pseudo Genes (internal stop)      :: 19 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15899
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039579-rid11723173.guided.113"
                     /strain="LGE2415"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8302 N 95.9628 W"
                     /collection_date="2015-08-24"
     gene            complement(251..796)
                     /gene="orn"
                     /locus_tag="J1Y83_004459"
     CDS             complement(251..796)
                     /gene="orn"
                     /locus_tag="J1Y83_004459"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418586.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoribonuclease"
                     /protein_id="HBA7632520.1"
                     /translation="MSANENNLIWIDLEMTGLDPERDRIIEIATLVTDANLNILAEGP
                     TIAVHQSDEQLALMDDWNVRTHTASGLVERVKASTMGDREAELATLEFLKQWVPAGKS
                     PICGNSIGQDRRFLFKYMPELEAYFHYRYLDVSTLKELARRWKPEILDGFTKQGTHQA
                     MDDIRESVAELAYYREHFIKL"
     gene            891..1943
                     /gene="rsgA"
                     /locus_tag="J1Y83_004460"
     CDS             891..1943
                     /gene="rsgA"
                     /locus_tag="J1Y83_004460"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313167.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small ribosomal subunit biogenesis GTPase RsgA"
                     /protein_id="HBA7632521.1"
                     /translation="MSKNKLSKGQQRRVNANHQRRLKTSKEKPDYDDNLFGEPDEGIV
                     ISRFGMHADVESADGDVHRCNIRRTIRSLVTGDRVVWRPGKPAAEGVNVKGIVEAVHE
                     RTSVLTRPDFYDGVKPIAANIDQIVIVSAILPELSLNIIDRYLVACETLQIEPIIVLN
                     KIDLLDDEGMEFVNEQMDIYRNIGYRVLMVSSHTQDGLKPLEEALTGRISIFAGQSGV
                     GKSSLLNALLGLQKEILTNDVSDNSGLGQHTTTAARLYHFPHGGDVIDSPGVREFGLW
                     HLEPEQITQGFVEFHDYLGLCKYRDCKHDTDPGCAIREAVEEGKIAETRFENYHRILE
                     SMAQVKTRKNFSDTDD"
     gene            2041..3009
                     /gene="psd"
                     /locus_tag="J1Y83_004461"
     CDS             2041..3009
                     /gene="psd"
                     /locus_tag="J1Y83_004461"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313166.1"
                     /note="Phosphatidylserine decarboxylase is synthesized as
                     a single chain precursor. Generation of the pyruvoyl
                     active site from a Ser is coupled to cleavage of a Gly-Ser
                     bond between the larger (beta) and smaller (alpha chains).
                     It is an integral membrane protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase"
                     /protein_id="HBA7632522.1"
                     /translation="MLNSFKLSLQYILPKLWLTRLAGWGASKRAGWLTKLVIDLFVKY
                     YKVDMKEAQKPDTASYRTFNEFFVRPLRDEVRPIDTDPNVLVMPADGVISQLGKIEED
                     KILQAKGHNYSLEALLAGNYLMADLFRNGTFVTTYLSPRDYHRVHMPCNGILREMIYV
                     PGDLFSVNHLTAQNVPNLFARNERVICLFDTEFGPMAQILVGATIVGSIETVWAGTIT
                     PPREGIIKRWTWPAGENDGSVALLKGQEMGRFKLGSTVINLFAPGKVNLVEQLESLSV
                     TKIGQPLAVSTETFVTPDAEPAPLPAEEIEAEHDASPLVDDKKDQV"
     gene            3031..6354
                     /gene="mscM"
                     /locus_tag="J1Y83_004462"
     CDS             3031..6354
                     /gene="mscM"
                     /locus_tag="J1Y83_004462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="miniconductance mechanosensitive channel MscM"
                     /protein_id="HBA7632523.1"
                     /translation="MRLIITFLMAWCLSWGAYAATAPDSKQITQELEQAKAAKPAQPE
                     VVEALQSALNALEERKGSLERIKQYQEVIDNYPKLSATLRAQLNNMRDEPRSVSPGMS
                     NDALNQEILQISSQLLDKSRQAQQEQERAREIADSLNQLPQQQTDARRQLNEIERRLG
                     TLTGNTPLNQAQNFALQSDSARLKALVDELELAQLSANNRQELARLRSELAEKESQQL
                     DAYLQALRNQLNSQRQLEAERALESTEQLAESSADLPKDIVAQFKINRELSAALNQQA
                     QRMDLVASQQRQAASQTLQVRQALNTLREQSQWLGSSNLLGEALRAQVARLPEMPKPQ
                     QLDTEMAQLRVQRLRYEDLLNKQPLLRQIHQADGQPLTAEQNRILEAQLRTQRELLNS
                     LLQGGDTLLLELTKLKVSNGQLEDALKEVNEATHRYLFWTSDVRPMTIAWPLEIAQDL
                     RRLISLDTFSQLGKASVMMLTSKETILPLFGALILVGCSIYSRRYFTRFLERSAAKVG
                     KVTQDHFWLTLRTLFWSILVASPLPVLWMTLGYGLREAWPYPLAVAIGDGVTATVPLL
                     WVVMICATFARPNGLFIAHFGWPRERVSRGMRYYLMSIGLIVPLIMALMMFDNLDDRE
                     FSGSLGRLCFILICGALAVVTLSLKKAGIPLYLNKEGSGDNITNHMLWNMMIGAPLVA
                     ILASAVGYLATAQALLARLETSVAIWFLLLVVYHVIRRWMLIQRRRLAFDRAKHRRAE
                     MLAQRARGEEEAHHHSSPEGAIEVDESEVDLDAISAQSLRLVRSILMLIALLSVIVLW
                     SEIHSAFGFLENISLWDVTSTVQGVESLEPITLGAVLIAILVFIITTQLVRNLPALLE
                     LAILQHLDLTPGTGYAITTITKYLLMLIGGLVGFSMIGIEWSKLQWLVAALGVGLGFG
                     LQEIFANFISGLIILFEKPIRIGDTVTIRDLTGSVTKINTRATTISDWDRKEIIVPNK
                     AFITEQFINWSLSDSVTRVVLTIPAPADANSEEVTEILLTAARRCSLVIDNPAPEVFL
                     VDLQQGIQIFELRIYAAEMGHRMPLRHEIHQLILAGFHAHGIDMPFPPFQMRLESLNG
                     KQTGRTLTSAGKGRQAGSL"
     gene            complement(6385..6699)
                     /locus_tag="J1Y83_004463"
     CDS             complement(6385..6699)
                     /locus_tag="J1Y83_004463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjeO family protein"
                     /protein_id="HBA7632524.1"
                     /translation="MSARMFILCCIWFVVAIPWILITSTLDKEWMIDEGGVKNICDVL
                     EYLENDDTRDVGVILTLPLFFPFFWFALWRKKRGWFMYATTLAIFGHWLWQFFLRYHF
                     CL"
     gene            complement(6696..7018)
                     /locus_tag="J1Y83_004464"
                     /pseudo
     CDS             complement(6696..7018)
                     /locus_tag="J1Y83_004464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000340721.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(7081..8583)
                     /gene="yjeM"
                     /locus_tag="J1Y83_004465"
     CDS             complement(7081..8583)
                     /gene="yjeM"
                     /locus_tag="J1Y83_004465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418580.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/gamma-aminobutyrate family transporter
                     YjeM"
                     /protein_id="HBA7632525.1"
                     /translation="MPHTIKKMSLIGLILMIFTSVFGFANSPSAYYLMGYSAIPFYIF
                     SALLFFIPFALMMAEMGAAYRKEEDGIYSWMNNSVGPRFAFIGTFMWFSSYIIWMVST
                     SAKVWVPFSTFLYGSDMTQHWHIAGLEPTQVVGLLAVAWMILVTVVASKGINKIARIT
                     AVGGIAVMCLNLVLLLVSITILLLNGGHFAQDINFLASPNPGYQSGLAMLSFVVFAIF
                     AYGGIEAVGGLVDKTENPEKNFAKGIVFAAIVISIGYSLAIFLWGVSTNWQQVLSNGS
                     VNLGNITYVLMKSLGVTLGNALHLSPEASLSLGVWFARITGLSMFLAYTGAFFTLCYS
                     PLKAIIQGTPKALWPEPMTRLNAMGMPSIAMWMQCGLVTVFILLVSFGGGTASAFFNK
                     LTLMANVSMTLPYLFLALAFPFFKARQDLDRPFVIFKTHMSAMIATVVVVLVVAFANV
                     FTIIQPVVEAGDWDSTLWMIGGPVFFSLLAMAIYQNYCSRMANKPELALD"
     gene            complement(8802..9779)
                     /gene="epmA"
                     /locus_tag="J1Y83_004466"
     CDS             complement(8802..9779)
                     /gene="epmA"
                     /locus_tag="J1Y83_004466"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000004771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P--(R)-beta-lysine ligase"
                     /protein_id="HBA7632526.1"
                     /translation="MSETASWQPSASIPNLLKRAAIMAEIRRFFADRGVLEVETPCMS
                     QATVTDIHLVPFETRFVGPGHSQGMNLWLMTSPEYHMKRLLVAGCGPVFQLCRSFRNE
                     EMGRYHNPEFTMLEWYRPHYDMYRLMNEVDDLLQQVLDCPAAESLSYQQAFLRYLEID
                     PLSADKTQLREVAAKLDLSNVADTEEDRDTLLQLLFTFGVEPNIGKEKPTFVYHFPAS
                     QASLAQISTEDHRVAERFEVYYKGIELANGFHELTDAREQQQRFEQDNRKRAARGLPQ
                     HPIDQNLIEALKVGMPDCSGVALGVDRLVMLALGAETLAEVIAFSVDRA"
     gene            10104..11912
                     /gene="frdA"
                     /locus_tag="J1Y83_004467"
     CDS             10104..11912
                     /gene="frdA"
                     /locus_tag="J1Y83_004467"
                     /EC_number="1.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase (quinol) flavoprotein
                     subunit"
                     /protein_id="HBA7632527.1"
                     /translation="MQTFQADLAIVGAGGAGLRAAIAAAQANPNAKIALISKVYPMRS
                     HTVAAEGGSAAVAQDHDSFEYHFHDTVAGGDWLCEQDVVDYFVHHCPTEMTQLELWGC
                     PWSRRPDGSVNVRRFGGMKIERTWFAADKTGFHMLHTLFQTSLQFPQIQRFDEHFVLD
                     ILVDDGHVRGLVAMNMMEGTLVQIRANAVVMATGGAGRVYRYNTNGGIVTGDGMGMAL
                     SHGVPLRDMEFVQYHPTGLPGSGILMTEGCRGEGGILVNKNGYRYLQDYGMGPETPLG
                     EPKNKYMELGPRDKVSQAFWHEWRKGNTISTPRGDVVYLDLRHLGEKKLHERLPFICE
                     LAKAYVGVDPVKEPIPVRPTAHYTMGGIETDQNCETRIKGLFAVGECSSVGLHGANRL
                     GSNSLAELVVFGRLAGEQATERAATAGNGNEAAIEAQAAGVEQRLKDLVNQDGGENWA
                     KIRDEMGMAMEEGCGIYRTPELMQKTIDKLAELQERFKRVRITDTSSVFNTDLLYTIE
                     LGHGLNVAECMAHSAMARKESRGAHQRLDEGCTERDDVNFLKHTLAFRDADGTTRLEY
                     SDVKITTLPPAKRVYGGEADAADKAEAANKKEKANG"
     gene            11905..12639
                     /locus_tag="J1Y83_004468"
     CDS             11905..12639
                     /locus_tag="J1Y83_004468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase/fumarate reductase
                     iron-sulfur subunit"
                     /protein_id="HBA7632528.1"
                     /translation="MAEMKNLKIEVVRYNPEVDTAPHSAFYEVPYDATTSLLDALGYI
                     KDNLAPDLSYRWSCRMAICGSCGMMVNNVPKLACKTFLRDYTDGMKVEALANFPIERD
                     LVVDMTNFIESLEAIKPYIIGNSRTADQGTNIQTPAQMAKYHQFSGCINCGLCYAACP
                     QFGLNPEFIGPAAITLAHRYNEDSRDHGKKERMAQLNSQNGVWSCTFVGYCSEVCPKH
                     VDPAAAIQQGKVESSKDFLIATLKPR"
     gene            12650..13045
                     /gene="frdC"
                     /locus_tag="J1Y83_004469"
     CDS             12650..13045
                     /gene="frdC"
                     /locus_tag="J1Y83_004469"
                     /EC_number="1.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdC"
                     /protein_id="HBA7632529.1"
                     /translation="MTTKRKPYVRPMTSTWWKKLPFYRFYMLREGTAVPAVWFSIELI
                     FGLFALKNGPEAWAGFVDFLQNPVIVIINLITLAAALLHTKTWFELAPKAANIIVKDE
                     KMGPEPIIKSLWAVTVVATIVILFVALYW"
     gene            13056..13415
                     /gene="frdD"
                     /locus_tag="J1Y83_004470"
     CDS             13056..13415
                     /gene="frdD"
                     /locus_tag="J1Y83_004470"
                     /EC_number="1.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdD"
                     /protein_id="HBA7632530.1"
                     /translation="MINPNPKRSDEPVFWGLFGAGGMWSAIIAPVMILLVGILLPLGL
                     FPGDALSYERVLAFAQSFIGRVFLFLMIVLPLWCGLHRMHHAMHDLKIHVPAGKWVFY
                     GLAAILTVVTLIGVVTI"
     gene            13478..14611
                     /locus_tag="J1Y83_004471"
     CDS             13478..14611
                     /locus_tag="J1Y83_004471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cephalosporin-hydrolyzing class C beta-lactamase
                     EC-5"
                     /protein_id="HBA7632531.1"
                     /translation="MFKTTLCTLLITASCSTFAAPQQINDIVHRTITPLIEQQKIPGM
                     AVAVIYQGKPYYFTWGYADIAKKQPVTQQTLFELGSVSKTFTGVLGGDAIARGEIKLS
                     DPTTKYWPELTAKQWNGITLLHLATYTAGGLPLQVPDEVKSSSDLLRFYQNWQPAWAP
                     GTQRLYANSSIGLFGALAVKPSGLSFEQAMKTRVFQPLKLNHTWINVPSAEEKNYAWG
                     YREGKAVHVSPGALDAEAYGVKSTIEDMARWVQSNLKPLDINEKTLQQGIQLAQSRYW
                     QTGDMYQGLGWEMLDWPVNPDIIINGSDNKIALAARPVKPITPPTPAVRASWVHKTGA
                     TGGFGSYVAFIPEKELGIVMLANKNYPNPARVAAAWQILNALQ"
     gene            14700..15233
                     /locus_tag="J1Y83_004472"
     CDS             14700..15233
                     /locus_tag="J1Y83_004472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin family protein"
                     /protein_id="HBA7632532.1"
                     /translation="MRLLPLVAAATAAFLVVACSSPTPPRGVTVVNNFDAKRYLGTWY
                     EIARFDHRFERGLEKVTATYSLRDDGGLNVINKGYNPDREMWQQSEGKAYFTGVPTRA
                     ALKVSFFGPFYGGYNVIALDREYRHALVCGPDRDYLWILSRTPTISDEVKQEMLAVAT
                     REGFDVSKFIWVQQPGS"
     gene            complement(15230..15547)
                     /locus_tag="J1Y83_004473"
     CDS             complement(15230..15547)
                     /locus_tag="J1Y83_004473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418572.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound-resistance protein
                     SugE"
                     /protein_id="HBA7632533.1"
                     /translation="MSWIILVIAGLLEVVWAVGLKYTHGFSRLTPSVITVTAMIVSLA
                     LLAWAMKSLPVGTAYAVWTGIGAVGAAITGIVLLGESANPMRLASLALIVLGIIGLKL
                     STH"
     gene            complement(15723..15869)
                     /gene="ecnB"
                     /locus_tag="J1Y83_004474"
     CDS             complement(15723..15869)
                     /gene="ecnB"
                     /locus_tag="J1Y83_004474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein toxin entericidin B"
                     /protein_id="HBA7632534.1"
                     /translation="MVKKTIAAIFSVLVLSTVLTACNTTRGVGEDISDGGNAISGAAT
                     KAQQ"
BASE COUNT         3974 a         4212 c         4087 g         3626 t
ORIGIN      
        1 ccttggcaag gtcgtgctct accaactgag ctattcccgc gtactactta attttgcttt
       61 cgtaattttc gcatttctgc gtcgttacgg gaggcgcatt atacgagaaa tctttccctc
      121 tgcaaccccc ctaaaaaatt ttttttgagt ttttatctca agtgctgaaa ttatcagcaa
      181 gacgttcaat ttagcgtcaa aatcacgccg ccgcaagtgg attccggcgc ttacgtgacc
      241 aggaaaaatt ttacagcttg ataaaatgct cgcggtagta agccaactcc gccaccgatt
      301 cacggatatc atccatcgcc tgatgcgtcc cctgcttggt aaagccgtca agaatttccg
      361 gcttccagcg gcgcgccagc tctttcaggg tgctaacatc gagatagcgg tagtggaagt
      421 aggcttccag ctccggcatg tatttaaaca ggaagcgacg gtcctgaccg atgctgttac
      481 cacaaatcgg cgattttccc gcaggcaccc actgttttaa gaattcgagc gttgccagtt
      541 cagcttcacg atcgcccatc gtgctcgctt tcacacgctc taccagcccg ctggcggtat
      601 gggtgcgcac gttccagtca tccatcagcg ccagctgttc atcggactga tgtactgcaa
      661 tggtcggccc ttctgccaga atattcaggt tggcatcggt caccagcgtg gcaatctcaa
      721 taatgcgatc gcgctcggga tccagaccgg tcatctcaag atcgatccaa atcaggttgt
      781 tttcattggc actcatgcta ttttccaccc attcacagtg actatattga tccacaattg
      841 cgtgtatcat agatgttttg cccatcaggg gcgaccagga gtctgtacga ttgagtaaaa
      901 ataaactctc caaaggccag cagcgccgcg tgaacgccaa tcaccagcgt cgtcttaaaa
      961 cgtctaagga gaagcccgac tacgacgaca atctgtttgg cgagcctgat gaaggtatcg
     1021 tcatcagccg ctttggtatg cacgctgatg tggagtccgc cgatggcgac gttcaccgct
     1081 gcaatattcg ccgtaccatc cgttcgttgg tgaccggcga ccgcgtagtc tggcgtccgg
     1141 gtaaaccagc ggcggaaggc gtaaatgtca aagggatcgt ggaagcggtg catgagcgca
     1201 cctcagtgtt gacgcgcccg gatttttacg acggtgtgaa acctattgct gccaacatcg
     1261 accagattgt cattgtctcc gccattttgc cggagctgtc gctcaatatt atcgaccgtt
     1321 acctggtggc ctgcgaaacc ttgcagattg agccgattat tgtgctcaac aagatagacc
     1381 tgctggacga cgaaggcatg gagttcgtca acgagcagat ggatatctac cgcaatatcg
     1441 gttatcgcgt attgatggtt tccagtcata ctcaggatgg gctgaaaccg ctggaagagg
     1501 cgttgaccgg gcgcatcagc atttttgccg ggcagtctgg cgttggcaaa tccagcctgc
     1561 tgaacgcgtt gctggggctg caaaaagaga tcctgaccaa cgatgtctct gacaactcgg
     1621 ggcttggtca gcacaccacg accgccgcac gactgtatca cttcccgcac ggcggtgatg
     1681 tgattgattc cccaggtgtg cgtgagtttg gtctctggca cctggagccg gaacaaatca
     1741 ctcagggctt tgtcgaattc catgactatt taggtctgtg taaatatcgc gattgcaaac
     1801 acgataccga tccgggctgc gctatccggg aagcggttga ggaagggaaa atcgcggaaa
     1861 cccgtttcga aaactatcac cgtattctgg aaagcatggc gcaggtaaaa acgcgtaaaa
     1921 acttttctga tacggatgac tgacaattaa gctaaccgtc gttagaatcg tccccctttt
     1981 tttcaggatc cggcatgtag gccggatcag gaacgacaaa acaatggcct ggaggctacc
     2041 ttgttaaatt catttaaact ttcgctacag tacattctgc cgaaactatg gcttactcgc
     2101 ctggcgggtt ggggcgcaag caagcgggca ggatggctga caaaactggt tatcgatctg
     2161 ttcgttaaat actacaaggt cgacatgaaa gaggcgcaaa agccggacac cgccagctac
     2221 cgcaccttta acgaattctt tgtccgtccg ctgcgtgacg aagtacgccc aatcgatacc
     2281 gatccgaatg tactggttat gcctgccgat ggcgttatca gccagttggg taaaatcgaa
     2341 gaagataaaa tcctgcaagc caaaggccac aactacagcc tcgaagccct gctggcaggc
     2401 aactatctga tggcggatct cttccgtaac ggtacgtttg tgaccactta cctctcccca
     2461 cgtgactacc accgcgtaca catgccgtgc aacggtattc tgcgtgaaat gatctacgtg
     2521 ccgggcgatc tcttctccgt taaccatctc acggctcaga acgtgccgaa tctgtttgcc
     2581 cgtaatgaac gcgtgatctg cctgttcgat accgaatttg gcccgatggc gcagattctg
     2641 gtcggtgcga cgattgtagg cagcattgag acggtctggg cgggcaccat tacgccgccg
     2701 cgcgaaggca tcatcaagcg ctggacctgg cctgccgggg aaaacgacgg ttctgtggca
     2761 ttgctgaaag gccaggaaat gggtcgattt aaacttggct ctaccgttat caacctgttt
     2821 gcaccaggta aagtgaacct ggttgagcaa ctggaaagcc tgtctgtcac gaaaattggc
     2881 cagccgctgg cagtatctac cgaaaccttt gttacgccag acgctgaacc agccccgctt
     2941 cctgctgaag agatcgaagc agaacacgac gccagcccat tggttgacga caaaaaagac
     3001 caggtctaat ccatcaaagg aaacgctgac gtgcgcctga ttatcacttt tctgatggcc
     3061 tggtgcctca gttggggggc gtacgccgcg acggcccccg atagcaaaca aatcacccag
     3121 gaactggagc aggcaaaagc ggcgaaaccc gcgcagccgg aagtcgtaga ggcgctccag
     3181 tctgccttaa atgcgcttga ggaacgaaaa ggttcccttg agcgcatcaa gcaatatcaa
     3241 gaagtcattg ataattatcc gaaactttcc gccactctgc gcgcgcagtt aaacaacatg
     3301 cgtgacgagc cacgcagcgt gtcgccggga atgtctaacg atgcgctgaa tcaggaaatt
     3361 ctccagatca gcagtcagtt gctggataaa agccgtcagg cccagcaaga gcaggagcgc
     3421 gcccgcgaga ttgccgattc gctgaatcaa ctgccgcaac agcaaaccga tgcccgccgt
     3481 cagttgaatg aaatcgagcg ccgcctgggg acacttaccg gcaatacccc gctcaatcag
     3541 gcacaaaatt tcgcgttgca gtctgactcc gcacgtctga aagcgctcgt tgatgaactg
     3601 gagctggcgc agctatctgc caataaccgc caggaattag cgcgcttgcg ctctgagctg
     3661 gcggaaaaag agagccagca gctggatgca tatttgcaag cactacgcaa ccagttgaat
     3721 agtcaacgcc aactggaagc ggaacgggcg ctggaaagta ccgagcaact ggcggaaagt
     3781 agcgccgatt tgccaaaaga tatcgtcgcc cagtttaaaa ttaaccgcga actatcggcg
     3841 gcgctgaatc aacaggcgca gcggatggat ctcgttgcct cgcaacagcg tcaggctgcc
     3901 agccagacgt tacaggtccg gcaggcgttg aatacgctgc gtgaacagtc gcaatggctg
     3961 ggatcatcta acctgctcgg cgaagcgttg cgggcgcagg tggcacggct accggaaatg
     4021 ccaaaaccac aacagcttga taccgaaatg gcgcagttac gtgtgcaacg gttacgttat
     4081 gaggatctgc ttaataaaca gccgctgcta cggcaaatcc atcaggccga cggtcagcca
     4141 ctgaccgctg agcaaaaccg tattctggaa gcacagctac gcactcagcg tgagttgctg
     4201 aactcattgt tgcagggtgg cgacacgcta ctgctggagc tgaccaagct gaaagtctcc
     4261 aacgggcaac tggaggatgc gctgaaagag gtgaacgaag cgacgcaccg ctatctgttc
     4321 tggacctctg acgtgcgccc gatgaccatc gcctggccgc tggaaatcgc ccaggatcta
     4381 cgtcgtctca tttcgctgga caccttcagt cagttgggca aagccagtgt gatgatgctg
     4441 accagtaaag agacgatttt gccgctgttt ggcgcgttga ttctggtcgg ttgcagtatt
     4501 tactcgcgcc gctatttcac ccgttttctt gaacgttcgg cggcgaaagt cggcaaagtg
     4561 actcaggatc acttctggct gacgttacgc actcttttct ggtcgattct cgtcgcgtca
     4621 ccgctgcctg tgctgtggat gacactgggt tacggcttgc gcgaggcgtg gccttatccg
     4681 ctggcggtcg cgattggtga tggcgtaaca gccaccgtgc cgctgctgtg ggtagtgatg
     4741 atttgcgcca cctttgctcg cccgaacggc ttgtttatcg ctcattttgg ctggccgcgc
     4801 gaacgtgttt cccgtgggat gcgttactac ctgatgagca tcgggcttat tgtgccgctg
     4861 attatggcgc tgatgatgtt cgataacctc gacgaccgtg aattctccgg ttcgctggga
     4921 cggctttgct ttatcctcat ttgcggtgcg ctggcggtgg tcaccctcag cctgaaaaag
     4981 gccgggatcc cgctgtatct caacaaagaa ggcagcggcg acaacattac caaccatatg
     5041 ctgtggaaca tgatgattgg cgcaccgctg gttgccattc tggcgtcggc ggtgggttat
     5101 ctggcaacgg cgcaggcgct gttagcgagg cttgaaacct cggttgccat ctggtttttg
     5161 ctactggtgg tttatcacgt tatccgccgc tggatgctga tccagcgccg caggctggcg
     5221 tttgaccggg caaagcatcg ccgggcagag atgttagcgc agcgtgcgcg tggcgaagag
     5281 gaagcgcatc atcacagtag cccggaagga gcaattgaag tcgatgaaag cgaagtcgat
     5341 ctcgatgcca tcagtgcgca atccttgcgg ctggtgcgct caattttgat gttgatcgcc
     5401 ctgctttcgg tcattgtgct gtggtcagaa atccattccg ctttcggctt cctcgaaaat
     5461 atttcgctgt gggatgtcac ttccacggta cagggcgtag aaagcctgga gccgattacc
     5521 ctcggtgcgg tactgattgc cattctggtg tttatcatca ccacgcagct ggtgcgcaac
     5581 ctgcccgcgc tgctggaact ggcgattctg cagcacctgg atttaacgcc gggtacgggc
     5641 tacgccatca ccaccatcac caaatatctg ctgatgctga ttggcgggct ggtcggcttc
     5701 tcaatgattg gtattgagtg gtcgaaattg cagtggctgg ttgccgcgct cggtgttggt
     5761 ctcggttttg gtttgcagga aattttcgcc aactttatct ctggcctgat catcctgttc
     5821 gaaaaaccga ttcgcattgg cgatacggta acgatccgcg atcttaccgg tagtgtgacg
     5881 aaaattaaca cccgcgccac caccatcagc gactgggacc gcaaagaaat tatcgtgccg
     5941 aacaaggcgt ttattaccga gcagtttatc aactggtcgc tctctgactc ggtcacgcgt
     6001 gtggtgttga caattccggc ccctgccgat gccaacagtg aagaagtgac ggaaatcctg
     6061 ctcaccgcag cgcgtcgctg ctcactggtg atcgacaacc cagcaccgga agtcttcctg
     6121 gtggatctgc agcaggggat tcagattttc gagctgcgta tttacgccgc tgagatgggt
     6181 caccgtatgc cgctacgcca tgagatccac cagctgattc tggctggctt ccacgcccac
     6241 ggtatcgata tgccattccc gcccttccag atgcgtctgg aaagcctcaa cggtaaacaa
     6301 acggggagaa cattaacttc tgcgggcaaa ggtcgtcagg cgggaagttt gtaaggtctt
     6361 tgtggccgga tgaacccatc cggctcacaa gcaaaaatga tagcgcagaa aaaactgcca
     6421 cagccagtgg ccgaagatcg ccagggtggt agcgtacata aaccagccgc gtttttttcg
     6481 ccacagcgcg aaccagaaaa aggggaaaaa gagcggtaat gtcaatatca caccgacatc
     6541 acgggtatcg tcattttcca gatactccag cacatcgcag atgtttttaa ccccgccttc
     6601 atcaatcatc cactctttgt ccagcgtgga agtgatgagt atccagggga tcgccacaac
     6661 aaaccagatg cagcacaaga taaacatgcg cgcactcatg agataatcgg tcgcttaatt
     6721 tcgctatagc gcatacgaaa caaatcgccg tatcccacta attctccgcg aaaaaccaga
     6781 catctaataa gataagaaga taagcgcaat agatatccgg caggtttccc caccgcggac
     6841 catcgcgctc tttgagggta ttaatagtgt tacccaccac catcgctact ttgcgggtgt
     6901 ggcgggtatc gacaaaagag aggaccgttt gctctatttc cgccacatcc ccggcagaaa
     6961 attgcagctc cctgatttca tcttcggtta tcactggatc ccgactagcg tcgtccataa
     7021 cgtcttcctt tccagacaac tcatcgccgg agagacgatg agttgcagga tttcatatga
     7081 tcagtcgaga gctaactcag gtttgttcgc catgcgactg caatagttct ggtaaatcgc
     7141 catcgctaac agcgagaaga agacagggcc gccaatcatc cacaatgtgc tgtcccagtc
     7201 tccagcttca accacaggtt gaatgatggt gaagacgttc gcaaatgcca ccaccagtac
     7261 gacaaccact gttgcaatca ttgccgacat atgcgttttg aaaatcacaa acggtctgtc
     7321 gagatcctga cgtgctttaa aaaatgggaa agccagcgcg aggaacaggt aaggaagcgt
     7381 catagacacg ttcgccatca gcgtcagctt gttaaagaac gccgatgcgg taccgccacc
     7441 aaacgaaacc agcaggatga agacagtaac caacccacac tgcatccaca tggcgataga
     7501 cggcatcccc atcgcattca ggcgcgtcat cggttccggc cacaatgctt tcggcgtccc
     7561 ctggatgatg gctttcagcg gtgaatagca aagcgtaaag aacgcaccgg tataagcgag
     7621 gaacatcgac agtccggtaa tacgcgcaaa ccatacgccc agcgacaatg acgcttcagg
     7681 tgacaaatgc agtgcgttac ccagcgtcac ccctaggctc ttcatcagca cataggtaat
     7741 attgccgagg ttaacggaac cattacttaa tacctgctgc cagtttgtgc tgacgcccca
     7801 taaaaatatt gccagcgaat aaccgattga aataacaata gcggcaaaaa caataccttt
     7861 ggcaaagttc ttttctggat tttccgtttt atcaaccaga ccaccaaccg cttcaattcc
     7921 gccataggca aaaatggcaa ataccacaaa cgatagcatt gccagaccgg actgataacc
     7981 tgggttcggt gatgcaagga aattaatatc ctgcgcgaaa tgcccaccat ttaataacaa
     8041 aatagtaatg cttactaaca gcaatactaa attcagacac attactgcaa taccgcccac
     8101 cgcagtaatg cgggcaattt tattaatccc ttttgaagca acgacggtga ccagaatcat
     8161 ccatgccact gccagcagac caaccacctg cgtaggctcc agtccggcaa tatgccagtg
     8221 ctgggtcatg tcgctaccgt agaggaatgt tgagaacggt acccaaactt tcgcggaggt
     8281 actcaccatc cagatgatat aagaggaaaa ccacataaac gtaccaatga aggcaaaacg
     8341 tggtccgaca ctattattca tccaggaata gataccatct tcttctttgc gataagcagc
     8401 tcccatttca gccatcatta aggcgaatgg aataaagaat aacaatgcag aaaatatata
     8461 aaagggaatc gcactataac ccattaagta ataagccgat gggctattgg caaatccaaa
     8521 tacggaagta aagatcatca atatgagtcc tatcagactc atctttttta tcgtgtgagg
     8581 catgaaacca tccttcattt cagcgcgtgt ataaaaggcg cccactgaac tgcactgccc
     8641 aacattgagc gggcagcgta aaaaccaata cagaattgat acctggatga tatcaaatat
     8701 ccagtcttaa attgtggcct ttagcggaaa aatgaaaaca tatgctacag aatggcgctt
     8761 atcacgccat tcttcgctgt taattcagta atttttcaga attatgcccg gtcaacgcta
     8821 aaggcgatga cttcagccag tgtctccgcg cccagcgcca acatcaccag acgatcaaca
     8881 cctaatgcca cgccggaaca gtcaggcata ccgactttca aggcttcaat cagattctgg
     8941 tcaatggggt gctgcggcaa accgcgcgcc gcgcgcttac ggttatcttg ttcaaagcgt
     9001 tgttgctgct cacgggcatc cgtcaattca tggaaaccat tcgccagctc aataccttta
     9061 taataaacct caaagcgttc agcgacccga tgatcttcgg tactgatttg cgccagtgat
     9121 gcctggctgg ctggaaagtg gtacacaaag gtcggttttt ctttgccaat atttggctct
     9181 acgccaaagg taaacagcaa ttgtagaagc gtgtcgcggt cttcttcagt atcggcaacg
     9241 ttgctcaaat ccagtttcgc tgccacttcc cgcagttgcg ttttgtcggc agatagtggg
     9301 tcaatttcca gataacgcaa gaaagcttgt tgataagaaa ggctttctgc tgccggacag
     9361 tccagcacct gttgcaagag atcgtccacc tcgttcatca accggtacat atcatagtgc
     9421 ggtcgatacc actcaagcat agtgaactca gggttgtgat aacgccccat ctcttcatta
     9481 cggaagctgc ggcacagctg gaataccggc ccacaaccgg caaccagcag gcgcttcata
     9541 tggtattccg ggctggtcat taaccagaga ttcatcccct gcgaatgccc ggggccaacg
     9601 aaacgtgtct caaacgggac caaatgaata tcggttaccg tcgcctggct catacaaggc
     9661 gtctccacct ccagcactcc acgatcggca aagaaacgac ggatctccgc cataatcgcc
     9721 gcgcgtttta ataagttagg aatggatgcg ctcggctgcc aggatgccgt ttcgctcata
     9781 gttaaatctc cagtttttga caagggcacg aagtctactc gcaacgcgac ggcgagacaa
     9841 attttacgca ggaataaaac aacggtgggc agtgactaaa aaaagcacga tctgatggtt
     9901 tagtaattaa attaatcatc ttcagtgata atttagccct cttgcgcact aaaaaaatcg
     9961 atctcgtcaa atttcagact tatccatcag actatactgt tgtacctata aaggagcagt
    10021 ggaatagcgt tcgcagaccg taactttcag gcacttaccc tgaagtacgg ggctgtggga
    10081 taaaaacaat ctggaggaat gtcgtgcaaa cctttcaagc cgatcttgcc attgtaggcg
    10141 ccggtggcgc gggattacgt gctgcaattg ctgccgcgca ggcaaatcca aatgcaaaaa
    10201 tcgcactaat ctcaaaagta tacccgatgc gtagccatac cgttgctgca gaagggggct
    10261 ccgccgctgt cgcgcaggat catgacagct tcgaatatca ctttcacgat acagtagcgg
    10321 gtggcgactg gttgtgtgag caggatgtcg tggattattt cgtccaccac tgcccaaccg
    10381 aaatgaccca actggaactg tgggggtgcc catggagccg tcgcccggat ggtagcgtca
    10441 acgtacgtcg cttcggcggc atgaaaatcg agcgcacctg gttcgccgcc gataagaccg
    10501 gcttccatat gctgcacacg ctgttccaga cctctctgca attcccgcag atccagcgtt
    10561 ttgacgaaca tttcgtgctg gatattctgg ttgatgatgg tcatgttcgc ggcctggtag
    10621 caatgaacat gatggaaggc acgctggtgc agatccgtgc taacgcggtc gttatggcta
    10681 ccggcggtgc gggtcgcgtt tatcgttaca acaccaacgg cggcatcgtt accggtgacg
    10741 gtatgggtat ggcgctaagc cacggcgttc cgctgcgtga catggaattc gttcagtatc
    10801 acccaaccgg cctaccaggt tccggtatcc tgatgaccga aggctgccgc ggtgaaggtg
    10861 gtattctggt caacaaaaat ggctaccgtt atctgcaaga ttacggcatg ggcccggaaa
    10921 ctccgctggg cgagccgaaa aacaaatata tggaactggg tccacgcgac aaagtttctc
    10981 aggccttctg gcacgaatgg cgtaaaggca acaccatctc cacgccacgt ggcgatgtgg
    11041 tttacctcga cctgcgtcac cttggcgaga aaaaactgca tgaacgtctg ccgttcatct
    11101 gcgaactggc gaaagcgtac gttggcgtcg atccggttaa agaaccgatt ccggtacgtc
    11161 cgaccgcaca ctacaccatg ggcggtatcg aaaccgatca gaactgtgaa acccgcatta
    11221 aaggtctgtt cgccgtgggt gaatgttcct ctgttggtct gcacggtgcg aaccgtctgg
    11281 gctccaactc gctggcagaa ctggtggtct tcggtcgtct ggccggtgaa caagcgacag
    11341 agcgtgcagc aactgccggt aatggcaacg aagcggcaat tgaagcgcag gcagctggcg
    11401 ttgaacaacg tctgaaagat ctggttaacc aggatggcgg cgaaaactgg gctaagatcc
    11461 gcgacgaaat gggcatggcc atggaagaag gctgcggtat ctaccgtacg ccggaactga
    11521 tgcagaaaac catcgacaag ctggcagagc tgcaggaacg cttcaagcgc gtgcgcatca
    11581 ccgacacttc cagcgtgttc aacaccgacc tgctctacac cattgagctg ggccacggtc
    11641 tgaacgttgc tgaatgtatg gcgcactccg caatggcacg taaagagtcc cgcggcgcgc
    11701 accagcgtct ggacgaaggt tgcaccgagc gtgacgacgt caacttcctc aaacacaccc
    11761 tcgccttccg cgatgctgat ggcacgactc gcctggagta cagcgacgtg aagattacta
    11821 cgctgccgcc agctaaacgc gtttacggtg gcgaagcgga tgcagccgat aaggcggaag
    11881 cagccaataa gaaggagaag gcgaatggct gagatgaaaa acctgaagat tgaggtggtg
    11941 cgctataacc cggaagtcga taccgcaccg catagcgcat tctatgaagt gccttatgac
    12001 gcaactacct cattactgga tgcgctgggc tacatcaaag acaacctggc accggacctg
    12061 agctaccgct ggtcctgccg tatggcgatt tgtggctcct gcggcatgat ggttaacaac
    12121 gtgccaaaac tggcatgtaa aaccttcctg cgtgattaca ccgacggtat gaaggttgaa
    12181 gcgttagcta acttcccgat tgaacgcgat ctggtggtcg atatgaccaa cttcatcgaa
    12241 agtctggaag cgatcaaacc gtacatcatc ggcaactccc gcaccgcgga tcagggtact
    12301 aacatccaga ccccggcgca gatggcgaag tatcaccagt tctccggttg catcaactgt
    12361 ggtctgtgct acgccgcgtg cccgcagttt ggcctgaacc cagagttcat cggtccggct
    12421 gccattacgc tggcgcatcg ttataacgaa gatagccgcg accacggtaa gaaggagcgt
    12481 atggcgcagt tgaacagcca gaacggcgta tggagctgta ctttcgtggg ctactgctcc
    12541 gaagtctgcc cgaaacacgt cgatccggct gcggccattc agcagggcaa agtagaaagt
    12601 tcgaaagact ttcttatcgc gaccctgaaa ccacgctaag gagtgcaaca tgacgactaa
    12661 acgtaaaccg tatgtacggc caatgacgtc cacctggtgg aaaaaattgc cgttttatcg
    12721 cttttacatg ctgcgcgaag gcacggcggt tccggctgtg tggttcagca ttgaactgat
    12781 tttcggcctg tttgccctga aaaatggccc ggaagcctgg gcgggattcg tcgacttttt
    12841 acaaaacccg gttatcgtga tcattaacct gatcactctg gcggcagccc tgctgcacac
    12901 caaaacctgg tttgagctgg caccaaaagc agccaatatc attgtaaaag acgaaaaaat
    12961 gggaccagag ccaattatca aaagtctctg ggcggtaact gtggttgcca ccatcgtaat
    13021 cctgtttgtt gccctgtact ggtaaggagc ctgagatgat taatccaaat ccaaagcgtt
    13081 ctgacgaacc ggtattctgg ggcctcttcg gggccggtgg tatgtggagc gccatcattg
    13141 cgccggtgat gatcctgctg gttggtattc tgctgccgct agggctgttt ccgggtgatg
    13201 cgctgagcta cgagcgcgtt ctggcgttcg cgcagagctt cattggtcgc gtattcctgt
    13261 tcctgatgat cgttctgccg ctgtggtgtg gtttacaccg tatgcaccac gcgatgcacg
    13321 atctgaaaat ccacgtacct gcgggtaaat gggttttcta cggtctggct gctatcctga
    13381 cagttgtcac actgattggt gtcgttacaa tctaacgcat cgccaatgta aatctggccc
    13441 gcctatggcg ggccgttttg tatggaaacc agaccctatg ttcaaaacga cgctctgcac
    13501 cttattaatt accgcctctt gctccacatt tgccgcacct caacaaatca acgatattgt
    13561 gcatcgcaca attaccccgc ttatagagca acaaaagatc cccggtatgg cggtagcggt
    13621 aatttatcag ggtaaacctt attactttac ctggggctat gcggacatcg ccaaaaagca
    13681 gcccgtcaca cagcaaacgt tgtttgagtt aggttcggtc agcaaaacat ttacgggcgt
    13741 gcttggtggc gacgctattg ctcgagggga aatcaagtta agcgatccca caacaaaata
    13801 ctggcctgaa cttaccgcta aacagtggaa tgggatcaca ctattacatc tcgcaaccta
    13861 caccgctggc ggcctgccat tgcaggtgcc ggatgaggtg aaatcctcaa gcgacttgct
    13921 gcgcttctat caaaactggc agcctgcatg ggctccagga acacaacgtc tgtatgccaa
    13981 ctccagtatc ggtttgttcg gcgcactggc tgtgaagccg tctggtttga gttttgagca
    14041 ggcgatgaaa actcgtgtct tccagccact caaactcaat catacgtgga ttaatgtacc
    14101 gtccgcagaa gaaaagaact acgcctgggg atatcgcgaa ggtaaggcag tacatgtttc
    14161 gccaggggcg ttagatgctg aagcttatgg tgtgaagtcg accattgaag atatggcccg
    14221 ctgggtgcaa agcaatttaa aaccccttga tatcaatgag aaaacacttc aacaagggat
    14281 acaactggca caatctcgct actggcaaac cggcgatatg tatcagggtc tgggctggga
    14341 aatgctggac tggccggtaa atcctgacat catcattaac ggcagtgaca ataaaattgc
    14401 actggcagca cgccccgtaa aaccgattac gcccccaact cctgcagtac gcgcatcatg
    14461 ggtacataaa acgggggcga ccggcggatt tggtagctat gtcgcgttta ttccagaaaa
    14521 agagctgggt atcgtgatgc tggctaacaa aaactacccc aatccagcga gagtcgccgc
    14581 cgcctggcag attctcaacg ctctacagta aaatttcatc gggtccgaat tttcggacct
    14641 tttctccgct tttccttgct gtcatctaca cttagaaaaa aaccagtaag gaaacattta
    14701 tgcgcctgct ccctctcgtt gccgcagcga cagctgcatt tctggtcgtt gcctgcagtt
    14761 cccctacgcc gccgcgtggc gtgaccgtag tgaataattt cgacgctaaa cgttatctcg
    14821 gtacctggta tgagattgcc cgttttgatc accgctttga acgtggactg gaaaaagtca
    14881 ccgcaacata cagcctgcgt gatgacggcg gcctgaatgt cattaataaa ggctataacc
    14941 ctgacagaga aatgtggcag cagagtgaag ggaaagcgta ctttaccggc gtaccaactc
    15001 gcgctgcgct gaaagtgtca ttctttggtc ctttctatgg cggttataac gttattgcac
    15061 tcgatcggga ataccgccat gcgctggttt gcggcccgga ccgcgactac ctgtggatac
    15121 tctcccgcac gccaaccatt tctgacgaag tgaaacagga gatgctggca gtcgcgaccc
    15181 gggaagggtt tgatgtcagt aagtttattt gggtacagca gcccggtagt taatgagtgc
    15241 tgagtttcag accaataatc cccaatacga ttaacgccag actcgccagg cgcatcgggt
    15301 tagcggactc accgagcagc acaatgccgg tgatggccgc gccgactgcg ccaatacccg
    15361 tccacacggc ataagccgtc cctactggta acgatttcat cgcccaggca agtagcgcca
    15421 gactgacaat catcgccgtc acggtaataa cacttggcgt caaacgacta aagccgtgag
    15481 tatatttcag gccaacggcc cataccactt ccagcagacc agcaataact aagataatcc
    15541 aggacatatc aggctccgga aaattggggc cgtccccggt gaaaagacgc gtttgcaggt
    15601 cgtcctgcaa agctgatggt tgaacggata ttttgccagg tggcagggga atttcaacta
    15661 ttacctatgt aactatcgca gaaaaaacaa acggcacgac acagctgtcg taccgttatt
    15721 gcttattgct gcgctttcgt tgctgcgcca gaaatcgcgt taccgccatc agaaatgtct
    15781 tcaccaacgc cacgcgtggt gttgcaggca gttaatactg ttgaaagcac cagaacagaa
    15841 aaaatcgctg caattgtctt cttcaccata acgtcttcct tttaggtaat gttatttat
//
LOCUS       DADSYM010000383        10176 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2415
            SAMN08039579-rid11723173.guided.158, whole genome shotgun sequence.
ACCESSION   DADSYM010000383 DADSYM010000000
VERSION     DADSYM010000383.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039579
            Sequence Read Archive: SRR6362533
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10176)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10176)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980162.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 03:39:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,484
            CDSs (total)                      :: 4,432
            Genes (coding)                    :: 4,347
            CDSs (with protein)               :: 4,347
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 43 of 85
            Pseudo Genes (incomplete)         :: 37 of 85
            Pseudo Genes (internal stop)      :: 19 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10176
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039579-rid11723173.guided.158"
                     /strain="LGE2415"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8302 N 95.9628 W"
                     /collection_date="2015-08-24"
     gene            complement(58..1797)
                     /gene="flhA"
                     /locus_tag="J1Y83_004475"
     CDS             complement(58..1797)
                     /gene="flhA"
                     /locus_tag="J1Y83_004475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system protein
                     FlhA"
                     /protein_id="HBA7632535.1"
                     /translation="MLSRSDLLTLLTINFIVVTKGAERISEVSARFTLDAMPGKQMAI
                     DADLNAGLINQAQAQTRRKDVASEADFYGAMDGASKFVRGDAIAGMMILAINLIGGVC
                     IGIFKYNLSADAAFQQYVLMTIGDGLVAQIPSLLLSTAAAIIVTRVSDNGDIAHDVRN
                     QLLASPSVLYTATGIMFVLAVVPGMPHFPFLMFSALLGFTGWRMSKRPQAAEAEEKSL
                     ETLTRTMTETSEQQVSWETIPLIEPISLSLGYKLVALVDKAQGNPLTQRIRGVRQVIS
                     DGNGVLLPEIRIRENFRLKPSQYAIFINGIKADEADIPADKLMALPSSETYGEIDGVL
                     GNDPAYGMPVTWIQPAQKAKALNMGYQVIDSASVIATHVNKIVRSYIPDLFNYDDITQ
                     LHNRLSSMAPRLAEDLSAALNYSQLLKVYRALLTEGVSLRDIVTIATVLVASSAVTKD
                     HILLAADVRLALRRSITHPFVRKQELTVYTLNNELENLLTNVVNQAQQAGKVMLDSVP
                     VDPNMLNQFQSTMPQVKEQMKAAGKDPVLLVPPQLRPLLARYARLFAPGLHVLSYNEV
                     PDELELKIMGALM"
     gene            1739..2527
                     /gene="lafU"
                     /locus_tag="J1Y83_004476"
     CDS             1739..2527
                     /gene="lafU"
                     /locus_tag="J1Y83_004476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011076207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative lateral flagellar export/assembly
                     protein LafU"
                     /protein_id="HBA7632536.1"
                     /translation="MVTTIKLIVNSVSKSERESIIAALHGQSIFNGGGLSPLNKISPS
                     HLPKPATVAVPEETEKKARDVNEKTALLKKKSATELGELATSINTIARDAHMEANLEM
                     EIVPQGLRVLIKDDQNRNMFERGSAKIMPFFKTLLVELAPVFDSLDNKIIITGHTDAM
                     AYKNNIYNNWNLSGDRALSARRVLEEAGMPENKVMQVSAMADQMLLDAKNPQSAGNRR
                     IEIMVLTKSASDTLYQYFGQHGDKVVQPLVQKLDKQQVLSQRAR"
     gene            2598..3653
                     /gene="dinB"
                     /locus_tag="J1Y83_004477"
     CDS             2598..3653
                     /gene="dinB"
                     /locus_tag="J1Y83_004477"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HBA7632537.1"
                     /translation="MRKIIHVDMDCFFAAVEMRDNPALRDIPIAIGGSRERRGVISTA
                     NYPARKFGVRSAMPTGMALKLCPHLTLLPGRFDAYKEASNHIREIFSRYTSRIEPLSL
                     DEAYLDVTDSVHCHGSATLIAQEIRQTIFNELQLTASAGVAPVKFLAKIASDMNKPNG
                     QFVITPAEVPAFLQTLPLAKIPGVGKVSAAKLEAMGLRTCGDVQKCDLVTLLKRFGKF
                     GRILWERSQGIDERDVNSERLRKSVGVERTMAEDIHHWSECEAIIERLYPELERRLAK
                     VKPDLLIARQGVKLKFDDFQQTTQEHVWPRLNKSDLIATARKTWDERRGGRGVRLVGL
                     HVTLLDPQMERQLVLGL"
     gene            3650..4102
                     /locus_tag="J1Y83_004478"
     CDS             3650..4102
                     /locus_tag="J1Y83_004478"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBA7632538.1"
                     /translation="MNNIQIRNYQPGDFQQLCAIFLRAVTMTASQHYSPQQIAAWAQI
                     DESRWKEKLAKSQVRVAVINAQPVGFITRIGHYIDMLFVEPEYTRRGVASALLKPLIK
                     SESELTVDASITAKPFFERYGFQTVKQQRVECRGTWFTNFYMRYKPQH"
     gene            4280..5431
                     /locus_tag="J1Y83_004479"
     CDS             4280..5431
                     /locus_tag="J1Y83_004479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015912462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA ligase RtcB family protein"
                     /protein_id="HBA7632539.1"
                     /translation="MEWYMGKYIRPLSDAVFTIASDDLWIESLAIQQLHTTANLPNMQ
                     RVVGMPDLHPGRGYPIGAAFFSVGRFYPALVGNDIGCGMALWQTDILARKYNADKFEK
                     RLSDLDDVAEESWLEENLPSAFAQHPWRNSLGSIGGGNHFAELQQIDQIIDAELFALA
                     GLDAQHLQLLVHSGSRGLGQSILQRHIASFSHHGLPEGSDDALRYIAEHDDALAFARI
                     NRQLIALRIMQQVKATGSPVLDVAHNFVSACQIGDQQGWLHRKGATPDDNGLVIIPGS
                     RGDYSWLVKPVANEKTLHSLAHGAGRKWGRTECKGRLAAKYTATQLSRTELGSRVICR
                     DKQLIFEEAPQAYKSAESVVQCLVLAGLIIPVARLRPVLTLKNSGGKKG"
     gene            5428..6042
                     /locus_tag="J1Y83_004480"
     CDS             5428..6042
                     /locus_tag="J1Y83_004480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000602105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor H"
                     /protein_id="HBA7632540.1"
                     /translation="MILLQLSSAQGPEECCLAVKKALDRLIKEATRQDVAVTVLETET
                     GRYSDTLRSALISLDGDNAWALSESWCGTIQWICQSPYRPHHGRKNWFLGIGRFTADE
                     QEQSDAIRYETLRSSGPGGQHVNKTDSAVRATHLASGISVKVQSERSQHANKRLARLL
                     IAWKLEQQQQENSAALKSQRRMFHHQIERGNPRRTFTGMAFIEG"
     gene            complement(6099..7556)
                     /gene="pepD"
                     /locus_tag="J1Y83_004481"
     CDS             complement(6099..7556)
                     /gene="pepD"
                     /locus_tag="J1Y83_004481"
                     /EC_number="3.4.13.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008783788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytosol nonspecific dipeptidase"
                     /protein_id="HBA7632541.1"
                     /translation="MSELSQLSPQPLWDIFAKICSIPHPSYHEEQLAEYIVGWAKEKG
                     FHVERDQVGNILIRKPATAGMENRKPVVLQAHLDMVPQKNNDTVHDFTKDPIQPYIDG
                     EWVKARGTTLGADNGIGMASALAVLADENVVHGPLEVLLTMTEEAGMDGAFGLQSNWL
                     QADILINTDSEEEGEIYMGCAGGIDFTSNLHLDREAVPAGFETFKLTLKGLKGGHSGG
                     EIHVGLGNANKLLVRFLAGHAEELDLRLIDFNGGTLRNAIPREAFATIAVAADKVDAL
                     KSLVDTYQEILKNELVEKEKNLALLLDSVANDKAALIAKSRDTFIRLLNATPNGVIRN
                     SDVAKGVVETSLNVGVVTMTDNNVEIHCLIRSLIDSGKDYVVSMLDSLGKLAGAKTEA
                     KGAYPGWQPDANSPVMHLVRETYQRLFNKTPNIQIIHAGLECGLFKKPYPEMDMVSIG
                     PTITGPHSPDEQVHIESVGHYWTLLTELLKEIPAK"
     gene            7817..8275
                     /gene="gpt"
                     /locus_tag="J1Y83_004482"
     CDS             7817..8275
                     /gene="gpt"
                     /locus_tag="J1Y83_004482"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HBA7632542.1"
                     /translation="MSEKYIVTWDMLQIHARKLASRLMPSEQWKGIIAVSRGGLVPGA
                     LLARELGIRHVDTVCISSYDHDNQRELKVLKRAEGDGEGFIVIDDLVDTGGTAVAIRE
                     MYPKAHFVTIFAKPAGRPLVDDYVVDIPQNTWIEQPWDMGVVFVPPISGR"
     gene            8367..9611
                     /gene="frsA"
                     /locus_tag="J1Y83_004483"
     CDS             8367..9611
                     /gene="frsA"
                     /locus_tag="J1Y83_004483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase FrsA"
                     /protein_id="HBA7632543.1"
                     /translation="MTQANLSETLFKPRFKHPETSTLVRRFSHGAQLPVQSALDGKTI
                     PHWYRMINRLMWIWRGIDPREILDVQARIVMSDAERTDDDLYDTVIGYRGGNWIYEWA
                     TQAMVWQQKACAEEDPQLSGRHWLHAATLYNIAAYPHLKGDDLAEQAQALSNRAYEEA
                     AQRLPGTMRQMEFTVPGGAPITGFLHMPKGDGPFPTVLMCGGLDAMQTDYYSLYERYF
                     APRGIAMLTIDMPSVGFSSKWKLTQDSSLLHQHVLKALPNVPWVDHTRVAAFGFRFGA
                     NVAVRLAYLESPRLKVVACLGPVVHTLLSDFKCQQQVPEMYLDVLASRLGMHDASDEA
                     LRVELNRYSLKVQGLLGRRCPTPMLSGYWKNDPFSPEEDSRLITSSSADGKLLEIPFN
                     PVYRNFDKGLQEITDWIEKRLC"
     gene            9669..10070
                     /locus_tag="J1Y83_004484"
     CDS             9669..10070
                     /locus_tag="J1Y83_004484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-binding protein Crl"
                     /protein_id="HBA7632544.1"
                     /translation="MTLPSGHPKSRLIKKFTALGPYIREGKCEDNRFFFDCLAVCVNV
                     KPAPEVREFWGWWMELEAQESRFTYSYQFGLFDKAGDWTSVQIKDAEVVERLEHTLRE
                     FHEKLRELLATLNLKLEPADDFRDEPVKLTA"
BASE COUNT         2430 a         2595 c         2730 g         2421 t
ORIGIN      
        1 cgtaggccgg ataaggcgtt cacgccgcat ccggcaatat gcaccgatgc cggatgccta
       61 catcaacgcc cccattatct tcaactccag ctcatccggc acttcgttat acgacagcac
      121 atgcagcccc ggcgcaaaca accttgcgta gcgcgccagc aaagggcgca actgcggcgg
      181 caccagcagc accggatctt tccccgccgc tttcatctgc tccttcacct gaggcatcgt
      241 gctctggaac tggttgagca tattcggatc cactggcacg ctatcaagca tcactttgcc
      301 ggcctgttgc gcctgattca ccacgttggt cagcagattt tccagctcat tattcagcgt
      361 atacaccgtc agctcctgct tgcgaacgaa cggatgggta atgctgcgcc gcagcgccag
      421 gcgcacatcg gccgccagca gaatatgatc tttagtcacc gcactactgg cgaccaacac
      481 ggtggcgatg gtgacgatat cgcgcaggga aacgccttca gtcagtagcg cccggtacac
      541 tttcagcaac tggctgtaat tgagcgccgc gctcaaatct tccgccagtc gcggtgccat
      601 cgacgacaaa cggttatgca gctgagtaat atcgtcatag ttaaacaaat caggaatata
      661 gctgcgcaca atcttattca catgggtggc aattacgctg gcgctgtcga tcacctgata
      721 ccccatattc agcgccttcg ctttctgcgc gggctgaatc caggtgaccg gcatcccgta
      781 cgccgggtcg ttccccagca cgccgtctat ctcgccgtag gtttcgctgg agggcagggc
      841 catcagttta tctgccggaa tatccgcttc atcagcctta atgccgttga tgaaaatggc
      901 gtactggctg ggcttgaggc ggaagttttc ccgaatacgg atctccggca gcagcacgcc
      961 gttgccgtcg gaaatcacct gacgtacgcc gcgaatacgc tgggtgagcg ggttgccctg
     1021 cgctttatcc accagcgcca ccagtttgta gccgaggctt aaactgatag gttcgatcag
     1081 cggaatagtt tcccaactga cctgttgctc gctggtttct gtcatcgtac gggttagcgt
     1141 ttcgaggctt ttctcttccg cctccgccgc ttgcgggcgc ttgctcatcc gccagccggt
     1201 aaaaccaagc agggcgctga acatcaggaa cgggaaatgc ggcattcccg gcaccaccgc
     1261 cagcacgaac ataattccag tggcggtgta gagaaccgac gggcttgcca gcaactgatt
     1321 acgcacgtca tgggcgatat caccgttatc gctgacgcgg gtgacgataa tcgccgccgc
     1381 ggtggagagc agcagggaag ggatttgcgc caccagccca tcgccgatag tcatcagcac
     1441 atattgctgg aaggcggcgt cggcgctcag gttgtatttg aagatgccga tacagacgcc
     1501 gccgatcagg ttgatcgcca gaatcatcat cccggcgatg gcgtccccgc gcacaaattt
     1561 tgatgccccg tccattgcgc cgtagaaatc ggcctcgctg gcgacatctt tacgccgggt
     1621 ctgcgcctgc gcctgattga tcaacccggc gttaaggtcg gcgtcaatcg ccatctgttt
     1681 gccgggcatc gcgtccaggg taaagcgggc agaaacctcg gaaatacgct cggccccctt
     1741 ggttacgacg ataaagttga ttgtcaacag cgtcagtaaa tccgagcgtg aaagcattat
     1801 cgccgcgctg cacgggcagt cgatttttaa cggtggtggg ttgtcgccgt taaataaaat
     1861 cagcccgtct catctgccaa aacctgccac cgtggcggtg ccggaagaga cggagaaaaa
     1921 agcgcgcgac gtcaacgaga aaacggcgct gttgaagaag aaatccgcca ccgagcttgg
     1981 cgagctggcg accagtatca ataccatcgc ccgtgatgcg catatggaag cgaatctgga
     2041 gatggagatt gttccccagg gattacgcgt gctgattaaa gacgaccaga accgcaatat
     2101 gtttgaacgc ggcagcgcga agattatgcc gttctttaaa actttgctgg tggagcttgc
     2161 gccagtgttc gactcgctcg ataacaaaat tattattacc gggcataccg atgcgatggc
     2221 ctacaaaaac aatatctaca acaactggaa cctttcgggc gatcgcgcgc tttcggctcg
     2281 tcgggtgctg gaagaggccg gaatgccgga aaataaagtg atgcaggtaa gcgcaatggc
     2341 ggaccagatg ctgctggatg ccaaaaatcc gcaaagcgcg ggcaaccggc gcattgagat
     2401 tatggtgctg accaaaagtg cgtccgatac gctgtatcaa tactttggtc agcatgggga
     2461 taaagtggtg cagccgctgg tgcaaaagct ggataagcag caggtgcttt cgcagcgagc
     2521 gcgttaaatg ctgaatcttt acgcatttct caaaccctga aatcactgta tactttacca
     2581 gtgttgagag gtgagcaatg cgtaaaatca ttcatgtgga tatggactgc tttttcgcag
     2641 cggtggagat gcgcgacaat cccgccctgc gcgatatccc tattgctatt ggcggcagcc
     2701 gcgaacgacg gggggtaatc agcaccgcca attatcccgc gcgtaaattt ggcgtacgta
     2761 gcgctatgcc gacagggatg gcgctcaaat tatgcccgca tctcaccttg cttccagggc
     2821 gctttgacgc ctacaaagaa gcctcaaatc atatccgcga aattttctcg cgctatacct
     2881 cgcgcattga accgttatca ctggatgaag cctatctcga tgtcaccgat agcgtccatt
     2941 gccacggttc tgcgaccctc atcgcccagg aaatccgcca gacgatcttc aacgagctgc
     3001 aactgacggc gtctgcgggc gttgcgccgg tcaagtttct cgccaaaatc gcctccgaca
     3061 tgaataaacc caacggtcag tttgtgatta ctccggctga ggttccggca tttctgcaaa
     3121 ccttaccgct ggcaaaaatt cccggcgtcg gcaaagtctc agcggctaaa ctggaagcga
     3181 tggggctacg aacctgcggt gatgtgcaaa agtgtgatct ggtgactctg ctcaaacgtt
     3241 ttggcaaatt tggccgcatt ttgtgggagc gtagtcaggg cattgacgag cgcgacgtta
     3301 acagcgaaag gttgcgaaaa tccgtcggtg tagaacgtac gatggcggaa gatattcatc
     3361 actggtctga atgtgaagcg atcatcgagc ggctttaccc tgaactggag cgtcgtttgg
     3421 caaaggtaaa acctgatttg ctgattgccc gccagggggt gaaattaaag ttcgacgatt
     3481 ttcagcaaac cacccaggaa cacgtctggc cgcggctgaa taaatctgat ctaatcgcca
     3541 ccgcgcgtaa aacctgggat gaacgccgcg gcgggcgcgg tgtgcgactg gtggggctgc
     3601 atgtgacgtt gctggacccg caaatggaaa gacaactggt gctgggatta tgaataacat
     3661 acaaataaga aactatcagc ctggtgattt tcagcaacta tgcgctattt tccttcgagc
     3721 tgttacgatg acggcgagtc agcactattc tccacagcaa attgccgcct gggcgcagat
     3781 tgacgaatct cgctggaagg agaaactcgc gaaatcacaa gtgcgggttg cagttattaa
     3841 tgcacaaccg gttggtttta ttacccgcat tggacattat atcgatatgt tatttgttga
     3901 accggaatac acccgacgcg gagttgccag cgctttgtta aaaccattga ttaagtctga
     3961 atccgaactt acggtggacg ccagcataac cgcaaaaccc ttttttgaac gttatggttt
     4021 tcagacagtt aagcagcagc gcgttgaatg ccggggaacg tggtttacta atttttatat
     4081 gcgatataaa ccgcaacatt aaatccagct tgcaatgaaa ataacgcccg tctagtatgt
     4141 gcgcgttttc ttattccaca aactgcaagg aggtaaatca tgacaaatcc tttatcaatg
     4201 actctttgca gacctttcca ggattaattc ttttttcttg ccctggattc gtctgccatt
     4261 tcctgatttt tattcataaa tggaatggta catgggcaaa tatattcgtc ccttatccga
     4321 tgcggtattt accatcgcat ctgatgacct gtggatcgag agtttagcga tccaacaatt
     4381 acacaccacg gcaaatttac ccaacatgca gcgcgttgtg gggatgccag atttacatcc
     4441 cggacgcggc tacccgattg gcgcagcgtt cttctctgtt ggtcgttttt acccggcact
     4501 ggtcggcaat gatatcggct gcggcatggc gctatggcaa acagatattc tcgctcgcaa
     4561 atacaacgcc gataagtttg aaaagcgatt atcagatctg gatgacgttg ctgaagaaag
     4621 ctggctggag gaaaacctgc cgtcagcatt tgcacagcat ccgtggcgca actcgcttgg
     4681 ctccatcggt ggcggtaacc acttcgcaga actgcaacaa attgatcaaa ttatcgacgc
     4741 ggaactgttt gcactggctg gtctggatgc gcagcatctg caactgctgg ttcatagcgg
     4801 ctcgagggga ttggggcagt ctattttaca gcggcatatt gcctcgtttt cgcatcatgg
     4861 tttgcctgaa ggcagtgacg acgcgctacg gtatattgcg gaacatgacg atgcgctggc
     4921 gtttgcgcgt attaatcgcc agctgatcgc tttgcgcata atgcaacaag ttaaggccac
     4981 cggaagtccg gttctggatg tggcgcataa ctttgttagc gcgtgccaaa tcggcgatca
     5041 acagggctgg ttgcatcgta aaggcgccac gccggatgac aacggtctgg tgattattcc
     5101 cggttcacgc ggcgattact cctggctggt taaacctgtt gcgaacgaaa aaacgctcca
     5161 ctcgctggcg catggggcgg ggcgtaaatg ggggcgcacc gagtgtaaag ggcgtctggc
     5221 agcgaaatac acggcgacgc aactctcgcg gactgaactt ggcagtcggg taatttgtcg
     5281 cgataaacaa ctcatctttg aagaagcgcc acaagcctat aaatcggctg aaagcgtggt
     5341 gcaatgtctg gtgctggctg ggttaattat tcctgtcgcg cgactgcgcc cggtgctgac
     5401 gctcaaaaac agtggaggga aaaaaggatg atcttgctac aactctcctc tgctcagggg
     5461 ccggaagaat gttgtctcgc agtgaaaaaa gcactggaca ggctgattaa agaagctacc
     5521 cgacaggacg tcgcggtaac ggtgctggaa acagaaacgg gtcgctactc tgacacactg
     5581 cgttcggcgc tgatttctct ggatggcgat aacgcatggg ctttaagcga aagctggtgc
     5641 ggcactattc agtggatttg tcagagtccg tatcggccac atcatgggcg caaaaactgg
     5701 tttctgggca ttgggcgttt taccgctgat gagcaggagc aatccgatgc gatccggtat
     5761 gagacgctgc gttcgtcggg gccgggcggt caacatgtca ataaaaccga ctcggcggta
     5821 cgcgccacgc atctggcatc cggaattagc gtgaaggttc agtcagagcg tagtcagcat
     5881 gccaacaaac ggctggcgcg attgcttatc gcctggaagc tggagcagca gcaacaggaa
     5941 aatagcgcgg cgctgaaatc gcagcggcga atgttccatc accagattga acgtggcaac
     6001 ccgcgacgaa cgtttacagg gatggctttt atcgaaggat aatgaaggat gaaactgccg
     6061 gaaggcgatt aaacgccatc cggcagcaaa tcaaataatt acttcgccgg aatttctttc
     6121 agcagttcag tcagaagcgt ccagtaatga ccaacgcttt cgatatgaac ttgctcatcc
     6181 ggggagtgtg gaccggtgat ggttggcccg atagacacca tgtccatttc cggatacggt
     6241 tttttgaaca gaccacattc gaggcccgcg tggataatct ggatgttcgg cgttttgttg
     6301 aacagacgct gataggtttc acgtaccaga tgcattaccg gagagttagc gtccggctgc
     6361 cagccaggat atgcgccttt tgcttcggtt ttcgcacctg ccagtttacc cagcgaatcc
     6421 agcatgctca ctacgtagtc tttaccgctg tcgatcagag aacggatcag gcagtgaatt
     6481 tctacgttat tgtcggtcat ggtcaccaca ccgacgttca gggaggtttc gaccacacct
     6541 tttgccacgt cggagttgcg gatcacaccg ttcggggtgg cgttcagcag acgaataaag
     6601 gtatcgcgag atttcgcaat cagggcagct ttatcgttcg caacagagtc cagcagcaat
     6661 gccagatttt tctctttctc taccagttcg tttttcagga tctcctgata ggtatccacc
     6721 agagatttca gggcgtcgac tttatcagct gcgacagcaa tggtcgcaaa ggcttcacgc
     6781 gggatggcgt tacgcagtgt gccgccgttg aaatcaataa gacgcaggtc cagttcttct
     6841 gcatgacccg ccaggaagcg caccaacagt ttgttggcat tacccaggcc aacgtggatt
     6901 tcaccgccgg agtgaccgcc tttcagacct tttaaggtta acttgaaggt ttcaaaacca
     6961 gctggaaccg cttcacgatc taaatgcagg ttggaggtga agtcgatacc tcccgcacaa
     7021 cccatgtaga tttcaccttc ttcttcggag tcggtgttaa tcagaatatc agcctgcaac
     7081 cagttgctct gtaagccaaa agcaccgtcc ataccggctt cttcggtcat ggtcagcagc
     7141 acttccagcg ggccgtgaac cacgttttcg tcagccagaa ccgccagtgc agaggccata
     7201 ccaatgccgt tatccgcacc cagcgtggtg ccgcgtgctt taacccattc gccatcaata
     7261 taaggctgga taggatcttt agtgaagtca tgcacggtgt cgttgttttt ttgcggcacc
     7321 atatcgaggt gggcctgcaa gacgaccggt ttacgatttt ccatacctgc ggtagcaggt
     7381 ttacgaatca ggatattacc tacctgatcg cgttcgacat ggaaaccttt ctcttttgcc
     7441 caaccaacaa tgtattcagc gagttgctct tcatgatagg acgggtgagg aatagaacag
     7501 attttggcaa aaatatccca cagcggctgt ggagataatt gagacagttc agacacgtta
     7561 agtctccttg tcgatcaccc gcaaaacagt attgcaggtc acagggttag cagaaaatgt
     7621 tgtcaacaca agacaggctt gcgagatatg tttgagaata ccactttatc ccgcgtcagg
     7681 gagaggcagt gcgtaaaaag acgcggactc atgtgaaata ctggttttta gtgcgtcaga
     7741 tctctataat ctcgcgcaac ctattttccc ctcgaacact ttttaagccg tagataaaca
     7801 ggctgggaca cttcacatga gcgaaaaata catcgtcacc tgggacatgt tgcagatcca
     7861 tgcacgtaaa ctcgcaagcc gactgatgcc ttctgaacaa tggaaaggca ttattgccgt
     7921 aagccgtggc ggtctggtac cgggtgcgtt actggcgcgt gaactgggta ttcgtcatgt
     7981 cgataccgtt tgtatttcca gctacgatca cgacaaccag cgcgagctta aagtgctgaa
     8041 acgcgcagaa ggcgatggcg aaggcttcat cgttattgat gacctggtag ataccggtgg
     8101 tactgcggtt gcgattcgtg aaatgtatcc aaaagcgcac tttgtcacca tcttcgcaaa
     8161 accggccggt cgtccgctgg ttgatgatta tgttgttgat attccgcaaa atacctggat
     8221 cgaacagccg tgggatatgg gcgtcgtatt cgtcccgcca atctccggtc gctaatcttt
     8281 tcaacgcctg gcactgccgg gcgttgttct ttttaacttc aggcgggtta caatagtttc
     8341 cagtaagtat tctggaggct gcatccatga cacaggcaaa cctgagcgaa accctgttca
     8401 aaccccgctt taaacatcct gaaacctcga cgctagtccg ccgctttagt cacggcgcac
     8461 aactgcctgt gcagtcggcc cttgatggta aaaccatccc tcactggtat cgcatgatta
     8521 accgtctgat gtggatctgg cgcggcattg acccacgtga aatcctcgat gtccaggcgc
     8581 gtattgtgat gagcgatgcc gaacgtaccg acgatgattt atacgatacg gtgattggct
     8641 accgcggcgg caactggatt tatgagtggg ctacccaggc gatggtgtgg caacaaaaag
     8701 cctgtgcgga agaagatccg caactcagcg gtcgacactg gctgcatgcg gctacgttgt
     8761 acaacattgc tgcctatcct catctgaaag gagatgacct ggccgagcaa gcgcaggctt
     8821 tgtcaaaccg cgcctatgaa gaggccgctc agcgtctgcc gggcacgatg cggcagatgg
     8881 agtttaccgt acccggcggt gcgcccatca ccggcttttt gcatatgccg aaaggcgatg
     8941 gcccgttccc gacagtatta atgtgtggtg gtctggatgc gatgcagacg gactattaca
     9001 gcctgtatga acgttatttt gcgccgcgcg gcattgctat gctgactatt gatatgccgt
     9061 cggtgggctt ttcttcaaaa tggaagctca cccaggactc cagcctgttg catcagcacg
     9121 ttttaaaggc gctgcctaac gtaccgtggg tggatcacac tcgcgtcgcg gcctttggtt
     9181 tccgtttcgg cgctaacgtt gccgtgcgtc tggcatacct tgaatcgccg cgtctgaaag
     9241 tggttgcctg tcttggtccg gtagttcata ccctgttgag tgattttaag tgccaacaac
     9301 aggtgccgga aatgtatctt gacgttctgg ccagtcgttt ggggatgcat gatgcttccg
     9361 acgaagcgtt gcgagtggag ttgaatcgct attcattaaa agtgcaagga ttgctgggac
     9421 gtcgctgccc aacgccaatg ttatcaggct actggaagaa cgatccgttc agcccggaag
     9481 aggactcacg cttaatcacc tcatcatctg ctgacggtaa attattagag atcccattta
     9541 acccagtgta tcggaatttt gacaaaggcc ttcaggaaat cacagactgg atcgaaaaac
     9601 gtttgtgtta aaaatttgct aaattttacc aatttggtaa aacagttgca tcacaacagg
     9661 agattgcaat gacgttaccg agtggacacc cgaagagcag attgatcaaa aaatttaccg
     9721 cactaggccc gtatattcgt gaaggtaagt gcgaagataa tcgattcttt ttcgattgtc
     9781 tggctgtatg cgtcaacgtg aaaccggcac cggaagtgcg tgagttctgg ggctggtgga
     9841 tggaactgga agcgcaggaa tcccgtttta cctacagtta ccagtttggt ctgttcgata
     9901 aagcaggcga ctggacgagt gttcagataa aagatgccga agtggttgaa cgactggagc
     9961 acaccctgcg tgaatttcac gagaagctgc gtgagctgct ggcgacgctg aatctgaaac
    10021 tggaaccggc agatgatttt cgtgatgaac cggtgaagtt aacggcgtga atgaaatgtg
    10081 ccggatgcga tgttagagca tctcttccgg cctgaaatat tggttttcgt agttttcgta
    10141 ggccggataa ggcgttcacg ccgcatccgg caatat
//
